LOCUS       DAOREF010000001        10677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_2819_length_10677_cov_5.263698, whole genome shotgun sequence.
ACCESSION   DAOREF010000001 DAOREF010000000
VERSION     DAOREF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2819_length_10677_cov_5.263698"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKI33_00005"
     CDS             complement(<1..253)
                     /locus_tag="PKI33_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HNM35406.1"
                     /translation="MNIEKQYQEDHSVKLVVEVEPATMEKYKKRAATKISSRGNIPGF
                     RPGKAPYAIVVRTYGEAAIAEQAIDLFIDAEYSNILKEAD"
     gene            complement(297..378)
                     /locus_tag="PKI33_00010"
     tRNA            complement(297..378)
                     /locus_tag="PKI33_00010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Leu,seq:tag)
     gene            600..1064
                     /gene="bcp"
                     /locus_tag="PKI33_00015"
     CDS             600..1064
                     /gene="bcp"
                     /locus_tag="PKI33_00015"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNM35407.1"
                     /translation="MPVTSGIPAPTFELLDDTNTLRKLSDYRGKNVVLYFYPKDDTPG
                     CTKEACNFRDDYSAYERAGVVILGISPDSVESHVKFKKKFQLQFPLLADDGHKVCDLY
                     QVWGPKKFMGKAYEGVLRTTFLIDADGNIKKVYEDVRPAEHSQELLAELGLK"
     gene            1333..2343
                     /gene="coxB"
                     /locus_tag="PKI33_00020"
     CDS             1333..2343
                     /gene="coxB"
                     /locus_tag="PKI33_00020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNM35408.1"
                     /translation="MAHFVIVGILVIVMTILTYFGLDAAGLATQMHPVAASAQAAPID
                     QLWHWEIIVISFLLSLIVVPIVYSLVVFRQKKGELKDGEHIEGNTALEIGWTVVPLLI
                     VVVFAYLGAYSLGEVRRVDPNAMVVNVKAQQFSWTFEYPEYGFITTQLYLPIDRQVVL
                     KMESADVIHSFWVPEFRIKQDVVPGRITEYRVTPSLEGSYKVRCAELCGTSHAYMEAP
                     VVVVNSDAYDAWIVEQIKIAEESKTPEGQGKLLATKNGCVGCHSADGTAMTGPTWFNL
                     YGSEVKLADGSTVTADDAFITESILQPTAKVVNGFPPVMPPYTLTDEEISYIIAYIKT
                     LK"
     gene            2365..4257
                     /locus_tag="PKI33_00025"
     CDS             2365..4257
                     /locus_tag="PKI33_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNM35409.1"
                     /translation="MKGFFSSALARGLFWQLIGTLVGAGLVTGIRALMGLNALDTFFF
                     TEPAWVLGGFFGALFFLAGSGVVSDWFKWARGIDTPDHHEDHHTGAAKYINVSLDHKV
                     IGVQYTITALLLIAIGGLFALIFRTELAASQLQFLTTDMKLFSQNGPQLYNTLMSLHG
                     MIMIVSILLGTAGFINYVVPLLLGAQDMAFPRLNAFSFWIAVPGAVLLLMSLFLGGFD
                     TGWTGYPPLSARAPVGMQMFFLGVFVAGWSSILGSLNVIVTVLRMRAKGMTAWRMPIL
                     IWASLATALIALTATQLIGLSFQLVMFQRLFGMGFFDPAKGGNPVLFQHLFWFYSHPA
                     VYVFILPGLGVISELLPVFVRKPLFGYRWIAMSSLGIALVGFVVWAHHMYTSGMNEYL
                     RVPFMYSTLLVAVPTGVKFFSWVATIWKGKISTPTPMLFVLGGIIVFLLGGLSGPPNA
                     TVSTDLQLQDTYWIVGHFHDTIFGGFVFPFFAAIYFWFPKATGRKMNETLGKIHFWLM
                     TPSFFALTLGMMYAGLLGMRRRIVDYDPALGIDSTHLVMTIGAFLIALSVLIFFFNFF
                     NSIKNGEKAEGNVWNSRSPEWQVPSPMPAHNYDKPFEVIGEPYDYGLNDGGFVKFIEP
                     AKNSKH"
     gene            4277..5068
                     /locus_tag="PKI33_00030"
     CDS             4277..5068
                     /locus_tag="PKI33_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNM35410.1"
                     /translation="MAHSQDKSSALGQGVVIFVYLAVLTLIEYFIAVTFNATALLLVV
                     AVIKAALVVYYYMHIYELNAESGEHEDENSYEYKTGTNRIGLWLFLLSDAFVFAGLLV
                     MRSNLLGLTRPHLNQFLGLGVTAVLLISSFFMNRGETAMKHGDVKAFMNNTIITFVLG
                     LGFLLGVVLVEWRLAAEEGLVASFGNPSVGPLGGVFYMMTGMHAFHVFTGLIFLFVVW
                     NNARKGVYSAEKHWGVEAAAVYWHFVDLVWIFFYPALYLIGTAVK"
     gene            5131..5742
                     /locus_tag="PKI33_00035"
     CDS             5131..5742
                     /locus_tag="PKI33_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNM35411.1"
                     /translation="MDKKLLWTGGGILAIVVIAGLLSFIFANPPSFRGTLYGEPFPSA
                     AEIELTKADGSIFRLSDQRGKVVLLFFGYTSCPDVCPTTLAEMKLVMDKLESDAEQVQ
                     VVFVSVDPDRDTPEKIQTYVEHFNPTFLGLSGSLSELEPIWSSYSITREAVESDSALG
                     VIINHTARLFLVDPQGQMHLSFAYQTPVDDIVHDIRLILDQEK"
     gene            5751..6236
                     /locus_tag="PKI33_00040"
     CDS             5751..6236
                     /locus_tag="PKI33_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35412.1"
                     /translation="MKRILWSFLLGLVLAAITTELAYFLLKKENREPGVIELVIPAGT
                     AEKVAAGQAPLEIPKDMRFVVGDTLKVINQDTENHQLGPLWIPANSSATQKLETAENL
                     VVECSFQAGSVIGITVQEPVTWRTRISGIFFAGFPLGMMFAVYSVVVVPKKKEDNEHP
                     S"
     gene            6238..6957
                     /locus_tag="PKI33_00045"
     CDS             6238..6957
                     /locus_tag="PKI33_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNM35413.1"
                     /translation="MFSRKWVLTTLLALAGTAVCIRLGIWQLDRLEQRRAFNHQVESM
                     RAAEILDLNREVPEDIASMEWRAVTITGKYDFENQVALRNQYNAGLYGFHIITPLLFD
                     GGAVLVDRGWIPAESDSSPAEWRMYDEDEKVTVTGQIRLGHGKPAFGGVADALPVDGS
                     KLNVWNNLDVDKISTQMPYPILEVYIQPNADVSDTVPPIPFQPTIELTEGSHFGYALQ
                     WFTFATILFVGYPFFLQKQDD"
     gene            6957..8273
                     /locus_tag="PKI33_00050"
     CDS             6957..8273
                     /locus_tag="PKI33_00050"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNM35414.1"
                     /translation="MKYSLKTSFASYTALLVVVVFALTVVGRLVTITGAAFVCEGWPL
                     CLPTAPIGYLKLMHLGLVGLASIVMFLVWRKAWREQRHHAILLPLTTITGVMFIGQAF
                     VGAIEVTRQYPTHIVVLHTLTAIGLWISLAMLVFVSGTLEKDGVADYQFDWRQRVKDF
                     FILSKPVIVLLLLVTTYGGLVAGGKAWPSASLTFWTLFGGALAAAGSSALNQYIDREL
                     DKNMQRTSKRPLADGRLTAAEGLSYGLALCLVSYYVMAGFVNLLAAMLSLAGIFYYVF
                     FYSVWLKKATVQNIVIGGGAGAIPPMVGWAAATGNLSLAAWILFAIIFMWTPPHFWAL
                     AIVRKNDYARAGVPMLPVVEGEEKTRKQILIYTVELIAVTLLLPIFNLAGMIYLISAI
                     VLGAFLFYAAWKVFREGGNKVAWMMYRWSSMYLAFIFLALILDSVL"
     gene            complement(8327..9751)
                     /locus_tag="PKI33_00055"
     CDS             complement(8327..9751)
                     /locus_tag="PKI33_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35415.1"
                     /translation="MNSENKSLEKFLRSISFLLTFFLLSRAPIDADLWWHLRAGQLMW
                     QKGEILLNDVFSYTKLGSNWVNAFWVSEVLLYWIHRIGGYFLLSFFVSLIGAITFLIV
                     FSSMKGSSLLRSFILILAVITAAPIWGPRPQILSFFLVAFLDLWLNQPSRHRWLLMPI
                     FALWANIHGGWIWGFLLVIAHIAGLFVNSFFVEDKKKLRTEGLHLIGWTVLSALAVGI
                     NPNGIYIWKLPFQQVNVSLQIQEWLSPDFHRLDFHPMLWMLFLLILLAPFAQKPLQMG
                     QILKTIGFAYLTFVAQRNVALFAIVAAPLLADWANNIIQLIPLSLNSPTGSSALKPAF
                     QKFINGFILIILTLTALGNLYVITQNSRIDSNYPVNGVAWLREHEPQGKLFNSYNWGG
                     YLLWTLPEYPVFIDGRADLYGNEILGQWQDVVNARENAEEILNTWQVNIVFIEPYWDI
                     VPVLKEKGWHVKYEDKSSIILIRP"
     gene            9911..>10677
                     /locus_tag="PKI33_00060"
     CDS             9911..>10677
                     /locus_tag="PKI33_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35416.1"
                     /translation="MPYFAQWFLAPLRWLPEYPFSWGVWSLFSVSGILILAYYTKANP
                     FLLLISFPALGQVWLGQVDIFIATGLVVFLFNKNPYLRGLGLIVALSKPQLTALPIFF
                     CLLMEEDPRLFLKLFLIPSIIILLSFIFFGVNWIPQWILETRSNLPIHVWRLASLDVW
                     KFGIFLTPIPILFREKRDRMLSSLLISALATPFYGVYSYLVFLLFEVKWWHVVLSFAW
                     MIGFLFLHDSAMRLAWILPLAMLVEMLYKKFKPKGIE"
BASE COUNT         2528 a         2339 c         2505 g         3305 t
ORIGIN      
        1 catctgcttc ttttaaaata ttggaatatt ccgcgtcgat gaacaagtca atggcctgct
       61 cggcgatggc tgcttcgcca taggtgcgca ctacaatggc gtagggcgcc ttgcccgggc
      121 gaaagccggg gatgttgccg cgcgaggaga tcttggtcgc tgcgcgcttc ttatatttct
      181 ccatcgtggc cggttccact tccacgacca atttgacgga atgatcttcc tgatattgtt
      241 tttcgatgtt caaagtgacc tctttcttta tcctgcttga agtgattatg atgtagtggg
      301 gatggtgggg gtcgaaccca cacgccttgc ggcacatgat cctaagtcat gcctgtctgc
      361 cagttccagc acatccccgg tttgtgtgct tacaggcggg cgcaattata ccgtagggaa
      421 tgcccgaatc gccatttttt tctgaggttt tttataagga gattgatcca tgccttaaag
      481 ccggttttct accgaaaatt ggagtgctgc ccaccaaata attgtgaaag ttttcctacc
      541 cttaaaatga ataaaggtat aataacgcgc atacttcttt ggagagagag tactttttta
      601 tgcccgttac ttctggcatt cctgccccta catttgaatt gcttgacgat accaatacct
      661 tacgcaaact ttccgattat cgtggaaaga atgtcgtctt atatttttat ccgaaagatg
      721 atacgcccgg ttgcacaaaa gaagcgtgca actttcgcga tgattactct gcctacgaaa
      781 gggcaggggt ggtgattcta ggcattagcc cagactccgt tgaatctcat gtgaagttta
      841 aaaagaaatt ccagcttcaa tttcccttgc ttgctgatga tggacacaaa gtttgcgacc
      901 tttatcaggt gtggggacct aaaaagttta tgggtaaagc atatgaaggt gttcttcgta
      961 caacgttttt gatcgacgct gacggaaaca tcaagaaagt atatgaagat gtgcgcccag
     1021 ctgaacacag ccaggaactt ttagcagagt taggcttgaa ataacgcatc tctacgacaa
     1081 cgtatcgtgt tatagcttga tatagctttt agattgccga cggtatccaa acaccgtcgg
     1141 catgatttgt gaaaatcgag gtttgtgcaa gttaagaata gctgtgcatt tgaccaagat
     1201 agcgtgacaa taaaaaggga ttttgttata atctgatatt gtgaaaattg agacaattct
     1261 aactgtattt gtccaatttt agttaatcat cggagctcat gccccgttca atcttttcag
     1321 gagagagagt atatggccca ttttgtaatt gtgggaatct tggtcattgt gatgaccatt
     1381 ctcacgtatt tcggtcttga tgctgctggg cttgcaaccc aaatgcatcc tgtggcagcc
     1441 agcgcacaag ctgctcccat tgatcagtta tggcattggg aaatcatcgt aatttccttc
     1501 ctactttcgc tgatcgttgt accgattgta tatagtttgg ttgtgtttcg ccaaaagaaa
     1561 ggcgaactta aggatgggga acatatagag gggaatactg cccttgaaat cggttggacg
     1621 gttgtcccat tactcatcgt ggttgttttt gcttatttag gcgcttattc acttggtgaa
     1681 gtccgccggg tagacccaaa cgccatggtt gtaaacgtca aagcccaaca attttcatgg
     1741 acttttgaat accccgaata tgggtttatt acaactcaac tctatctgcc cattgaccgc
     1801 caggttgtgc ttaagatgga gtctgcggat gtgatccatt cattctgggt tcctgagttc
     1861 cgcatcaaac aagacgttgt acctggacgc atcaccgagt atcgtgttac tccgagtctt
     1921 gagggcagct acaaagtccg ctgtgccgag ctttgcggta catcccatgc ttatatggaa
     1981 gcccctgttg tcgttgtcaa ttcagatgcg tatgatgcct ggattgttga acaaatcaag
     2041 attgccgaag agtccaagac ccccgaaggg cagggtaaac tccttgcaac caagaatggc
     2101 tgtgttggtt gtcactctgc agacggtacg gctatgacag gaccgacttg gttcaacctg
     2161 tatggctcag aggttaagct tgctgacggc tccactgtga ctgccgacga tgccttcatc
     2221 acagagtcta ttcttcagcc aacagctaag gttgttaatg gcttcccgcc cgttatgccg
     2281 ccttacactt tgaccgatga agagatctct tatatcatcg catacatcaa gaccttgaaa
     2341 taaatttgat aaggattaaa tactatgaag ggattctttt ctagtgccct tgctcgcggt
     2401 ttgttttggc aattgatcgg cacgctcgtt ggtgctggtc ttgtcactgg cattcgcgca
     2461 ttgatggggt tgaatgcgct tgatacgttc ttctttaccg aaccggcctg ggtgttgggc
     2521 ggctttttcg gtgcgctttt cttcctcgct ggttctgggg ttgtgagcga ttggttcaag
     2581 tgggcgcgtg gcattgatac gccagatcac catgaggacc accacaccgg cgcggcaaag
     2641 tacatcaacg tttcacttga ccacaaggtc attggtgttc agtacaccat cacagcgttg
     2701 ttgttgatcg ccattggcgg tttatttgct ttgatctttc gtacagaatt ggctgcatct
     2761 caactccaat tcctcactac tgacatgaaa ttgttcagcc agaatggacc tcagttgtac
     2821 aacacattga tgtccttgca tggcatgatc atgatcgtct cgatcttgtt gggcacagct
     2881 ggcttcatca actatgtcgt tcccctgttg ctcggtgcgc aggatatggc attcccgcgc
     2941 ctcaatgcct tctcgttctg gattgctgtc cctggggctg tgcttctgct catgagcctt
     3001 ttcctcggcg gttttgatac tggttggacg ggttacccgc ctctctctgc ccgcgctcct
     3061 gtcggtatgc agatgttctt ccttggcgtg ttcgttgccg gttggtcatc cattcttggt
     3121 tctttgaacg ttattgttac tgtgcttcgt atgcgtgcca aaggcatgac cgcttggcgc
     3181 atgccgatcc tcatttgggc atctcttgcc actgcactga tcgcactgac cgctacacag
     3241 ttgatcggtc tttctttcca gttggtgatg ttccaacgtc tctttggaat gggcttcttt
     3301 gaccctgcca aaggcggcaa cccggttctc ttccaacacc tgttctggtt ctactctcac
     3361 cctgcggtgt acgtattcat tttgcctggc ttgggtgtga tctctgaatt actgcctgtg
     3421 tttgtccgca agcccctctt cggttatcgc tggattgcga tgtcctcttt gggcattgct
     3481 ttggtgggct tcgttgtgtg ggcgcaccac atgtatacct ctggtatgaa tgaatattta
     3541 cgcgtacctt tcatgtacag caccttgttg gtggctgtac cgacaggcgt taagttcttc
     3601 tcctgggttg caacaatttg gaaaggcaag atttcaactc ctaccccaat gctctttgtg
     3661 ttgggcggta ttatcgtctt ccttttaggt ggtctctcgg gtccccccaa tgcgacagtc
     3721 tctaccgacc ttcagttaca agacacctat tggatcgtag ggcacttcca cgacaccatt
     3781 ttcggtgggt ttgtcttccc attctttgct gccatctact tctggttccc caaagcaacc
     3841 ggacgcaaga tgaacgaaac ccttggcaag attcatttct ggctgatgac cccttcgttc
     3901 tttgccttaa ccttgggcat gatgtatgct ggcttgctcg gtatgcgccg ccgcatcgtg
     3961 gactatgacc ctgctttggg cattgactca acacatttag ttatgaccat tggtgcgttc
     4021 ttgatcgccc tttccgtttt gattttcttc ttcaacttct tcaatagcat caaaaatgga
     4081 gagaaggcgg aaggcaatgt ttggaattcg cgctcccctg aatggcaggt tccttccccc
     4141 atgcctgccc ataactacga taagccattc gaagtgatcg gcgaaccgta tgactacggt
     4201 ttgaatgatg gtggctttgt taagttcatt gaacctgcca agaatagcaa gcactaaagg
     4261 cgaggagagt aattaaatgg cacattcaca agataaatcg tccgctcttg gtcagggcgt
     4321 ggtgatcttt gtatatttgg cagttctgac ccttattgaa tacttcattg ctgtaacatt
     4381 caacgcaaca gcccttttgc tggttgtggc agtgatcaaa gccgctttgg ttgtgtatta
     4441 ctacatgcac atctatgagt tgaatgcaga aagcggcgag catgaagatg aaaactctta
     4501 tgagtataag accggcacaa atcgtatagg tctttggctg ttcctcttgt cagacgcatt
     4561 tgtgtttgct ggtctgttgg tgatgcgttc taacttgctt gggcttaccc gcccgcacct
     4621 caaccagttc ttgggtttgg gtgtaacggc tgtgttgctc atatcttcct tcttcatgaa
     4681 ccgtggcgag actgctatga aacatggcga tgtgaaagcc tttatgaaca acaccatcat
     4741 cacctttgtt ttgggcttgg gctttttgct tggcgtggtg ctcgtggaat ggcgcttggc
     4801 agctgaagaa ggtttggtgg cttcatttgg caacccgtct gtcggtccgt tgggcggcgt
     4861 tttctacatg atgaccggta tgcatgcctt ccatgttttc actggtttga tcttcctctt
     4921 tgttgtgtgg aataacgccc gcaaaggtgt gtactctgct gaaaaacatt ggggcgtaga
     4981 agccgctgca gtctattggc acttcgtaga tttagtttgg attttcttct accctgcctt
     5041 gtatctgatc ggcacggctg taaaataaaa tgaatacctc cgccgccgat gaggcggcgg
     5101 ttttttttcg acattaatcg gagataaaat atggataaaa aattactgtg gacgggcgga
     5161 ggcatccttg ctattgttgt aatagcaggg ttgctttctt ttatctttgc gaacccgccc
     5221 tcctttcgcg gcacactata tggtgagcct ttcccgtctg cagctgagat tgagttgaca
     5281 aaagcagatg gaagcatatt tcgcctaagt gaccagcgcg gaaaagttgt gctgctgttt
     5341 ttcggctaca cttcttgccc agatgtctgc ccaaccactc ttgccgaaat gaaattggtg
     5401 atggataaac tcgaaagtga cgcggaacaa gtacaggttg tttttgtgtc cgtggaccca
     5461 gaccgcgata ctccagaaaa gattcagacc tatgtagaac atttcaatcc caccttcttg
     5521 ggtctatcgg gatctttatc tgaacttgag cctatctgga gtagttattc cattacacgc
     5581 gaagccgtag aaagtgactc ggcactgggt gtaattatta accataccgc acgtttattc
     5641 cttgtcgacc cacaagggca gatgcatctt tcctttgcat accaaacccc tgtagatgat
     5701 attgtccacg atatccgttt gattttggat caagaaaaat gaatttcaca atgaagcgaa
     5761 ttttatggtc atttctttta gggcttgtat tggcagctat tacaacagag ctggcatact
     5821 ttctcttgaa aaaagaaaac cgcgagcccg gtgttattga attggtcatt cctgcgggaa
     5881 ctgctgaaaa agtcgctgct ggtcaggctc cgcttgaaat accgaaagat atgcgatttg
     5941 tcgtaggcga tacattgaaa gtgataaacc aagacactga aaatcatcaa ctgggtccgt
     6001 tatggattcc tgctaattct tctgctacac aaaaattgga aacagcagaa aatttagtag
     6061 tggaatgttc tttccaagcc gggagtgtga tcggtattac tgttcaagaa cctgttacat
     6121 ggcgcacaag gatttcaggc attttctttg ctggctttcc gcttggaatg atgtttgctg
     6181 tttacagtgt agtggttgtt cccaagaaga aagaagacaa tgaacatcct tcgtaaaatg
     6241 ttttcgagaa aatgggtgct tactacattg ctggctttag ctggtacggc ggtttgcatt
     6301 cgtttgggaa tttggcaact agatcgcctt gaacaacgcc gcgcatttaa tcatcaagtg
     6361 gaaagtatgc gcgcggcaga aatattagac ctcaatcgtg aagtccctga agacattgct
     6421 tcaatggaat ggcgggcggt taccattacc ggcaaatatg actttgagaa tcaagtcgct
     6481 ttgcgaaacc aatataatgc aggcttatac ggattccaca tcatcacccc gcttcttttc
     6541 gacgggggag ctgttctggt agaccgtggc tggatccccg ctgaaagcga ctcgtcacct
     6601 gctgagtggc gtatgtacga tgaagatgaa aaggtcacag tgacaggaca gatacggctt
     6661 gggcatggca aaccagcctt cggcggagtc gctgatgcgt tgcccgtaga tggatccaaa
     6721 ctcaatgtat ggaataatct cgatgtggat aaaatatcta cacaaatgcc ttatccaatt
     6781 cttgaggttt atattcagcc caatgctgat gtcagtgata cggttccgcc gatcccattt
     6841 cagcccacaa ttgaattgac ggaaggctca catttcgggt atgctttgca gtggtttacc
     6901 tttgcgacga ttttgttcgt aggttaccca ttttttctgc aaaaacagga cgattgatga
     6961 aatattcttt gaaaacttct tttgctagct acacagcctt gttggttgtc gtagtttttg
     7021 cgctcactgt ggtggggcgt ttggtgacca ttactggggc tgcctttgtg tgtgaaggtt
     7081 ggcctttatg tttgcctact gctccgattg gctatttaaa attgatgcat ttgggcttgg
     7141 ttggtttggc atccattgtg atgttcctcg tgtggcgaaa ggcttggcgt gaacagcggc
     7201 atcatgccat cttgttgcca ttgacgacga tcacaggcgt tatgtttatt gggcaggcat
     7261 ttgtaggggc aattgaggtg acgcgccagt acccaaccca tatcgtggtt cttcatacac
     7321 taacagcaat cgggctttgg atttcgttgg caatgcttgt ttttgtgtct ggcacacttg
     7381 agaaagatgg cgtggcagat tatcaatttg attggcgcca acgggtcaaa gactttttca
     7441 tcttatccaa gcctgtgatt gttttgttgc ttttggtgac aacctatgga ggcttagtgg
     7501 ctggtggtaa agcctggccc tctgcttcgc tcacattctg gacgttattc ggtggcgctt
     7561 tggctgctgc tggctccagc gctttgaacc aatatattga ccgtgaacta gataagaaca
     7621 tgcagcgcac atcgaagcgt ccgctggcag acgggcgttt gactgcggca gagggtcttt
     7681 catatggatt ggcgctttgt ttggttagtt attatgtaat ggctggtttt gtgaatttgc
     7741 tagcagccat gctttcgctg gcaggaattt tctactatgt attcttctac agcgtttggc
     7801 tgaagaaagc cactgttcaa aatatcgtga tcggtggtgg ggcaggggca attccgccaa
     7861 tggtgggttg ggctgccgca actgggaatc tcagtctcgc cgcatggatt ctctttgcca
     7921 tcattttcat gtggacacct ccacatttct gggcgcttgc cattgtccgc aaaaatgatt
     7981 atgcccgtgc cggtgttcct atgctccctg tagtcgaagg cgaagagaag actcgtaagc
     8041 agatcctaat ctatactgtt gaactgattg ctgtaactct tttgctcccc atctttaatt
     8101 tagctggaat gatttacctg atttcagcta ttgtcttggg cgctttcctc ttctatgctg
     8161 catggaaagt gttccgagaa ggtggaaata aggttgcttg gatgatgtat cgctggtcaa
     8221 gcatgtatct ggcgtttatc tttctggcat tgatcttgga ttcggtgttg taacctgtat
     8281 ttgatactaa aaaggctagc taataaaatt ggctagcctt tttcttttat gggcggatga
     8341 ggatgattga agatttatct tcgtatttta cgtgccatcc cttttctttc aaaacaggga
     8401 ctatatccca gtagggttcg ataaatacga tattaacctg ccaagtattt aatatttctt
     8461 cggcattttc cctggcgtta acaacatctt gccattgacc tagtatttca tttccgtata
     8521 aatctgcacg cccatcaata aacacaggat attcaggtaa tgtccataaa agatagccac
     8581 cccaattata agagttgaac agtttgcctt gtggttcatg ctctcttaac caagctactc
     8641 cattaacagg gtaattgcta tctattcgac tattttgagt aatgacgtaa agatttccta
     8701 gcgcagttaa tgtaagaatg atcaatataa atccattaat aaatttttga aatgccggtt
     8761 ttaatgctga agaccctgtt ggcgaattaa gacttaacgg gattaactgg ataatattat
     8821 ttgcccaatc tgctaatagt ggtgctgcaa caattgcaaa taaggcaacg tttctctgcg
     8881 cgacaaatgt gagatacgca aacccaatgg ttttgagtat ttgccccatc tgtagcggct
     8941 tttgcgcaaa tggtgccaaa agaattaata aaaagagcat ccacaacatg gggtggaaat
     9001 caagtcgatg aaaatcgggc gataaccact cttggatttg taatgagaca tttacttgtt
     9061 ggaagggcaa tttccatata tatatgccat ttggatttat acctaccgct aaggcagaaa
     9121 ggactgtcca cccaattaag tgcagccctt ctgtacgaag cttcttctta tcctcaacaa
     9181 aaaaggaatt gacaaacaac ccggcaatat gagctataac gagcaggaag ccccaaatcc
     9241 aaccgccatg aatgtttgcc caaagcgcaa atattggcat cagtagccat cgatgccgtg
     9301 atggttgatt tagccataag tctaaaaatg ccactagaaa gaaagaaagt atttgtgggc
     9361 gaggtcccca aatgggtgct gccgtaatca cagccaatat tagaataaaa ctccttaaga
     9421 gcgagctccc tttcatgctt gagaatacga tgagaaatgt tatagctccg ataagcgaaa
     9481 caaaaaagct tagtaagaaa tacccgccaa tccggtgtat ccaatataat aaaacctcag
     9541 aaacccagaa agcatttacc caatttgaac ctagcttggt gtagctaaag acgtcattta
     9601 atagaatttc tcccttttgc cacatcaatt gccctgccct taaatgccac cacaaatctg
     9661 catcaatggg tgcccggctt aataaaaaaa atgtcaataa aaaagaaata gacctaagga
     9721 atttttcgag agatttattt tctgagttca tggaggcgtt ttgagaacat ccgattctaa
     9781 taaaataatg tgggtagccg caattggtgc ggcaattggg ctaatcgcag cttcgtttta
     9841 tttacccggc ggcgatgacc tatatcgata ctatctgcct tttgcgcaag ggtgtcttca
     9901 ttgtggcttt atgccgtatt ttgcgcaatg gtttcttgct ccactacggt ggttaccaga
     9961 atatccattt tcctggggcg tttggtcttt gtttagcgta tctggcatcc ttatacttgc
    10021 ttattataca aaagctaacc cgtttttgtt attgatttcc tttcctgcgc ttggtcaagt
    10081 ttggcttggg caagtagata ttttcattgc aacagggcta gtcgtttttc tttttaataa
    10141 aaatccttat ttgcgtggtt tgggtctaat tgtcgcttta tctaaacctc aattgacggc
    10201 tttgccaatt ttcttttgcc ttttgatgga agaagatccg cgcctctttc ttaaactttt
    10261 cctgattccg tccatcatca ttttgcttag ttttatcttt tttggcgtga attggatccc
    10321 acagtggata ttagaaacgc gctcaaattt accgattcat gtctggcgct tggcaagctt
    10381 agatgtgtgg aagttcggaa tatttttgac gccaatacct attctgttcc gcgaaaaaag
    10441 agaccgcatg ttatctagct tgttgatatc tgcgttagct actccgtttt atggcgttta
    10501 ttcttatttg gtttttcttc tttttgaagt gaaatggtgg cacgtagtgc tttcttttgc
    10561 ttggatgatt gggtttttat ttttgcatga ctccgcaatg cgcctggcat ggattttgcc
    10621 tttggcaatg ctagttgaaa tgctttataa aaaattcaaa cctaaaggta tagagga
//
LOCUS       DAOREF010000002         9881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_3165_length_9881_cov_4.629147, whole genome shotgun sequence.
ACCESSION   DAOREF010000002 DAOREF010000000
VERSION     DAOREF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_9881_cov_4.629147"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKI33_00065"
     CDS             <1..443
                     /locus_tag="PKI33_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM35417.1"
                     /translation="SLAMGIGLLIAPPLADRTGKIQLVVITQALSIPFLILLGFSPIF
                     WVGAVSYYIRLALMNMSSPVYQTFVMEHVEPSGRATVASLTSMAWNFGWAFSPTISGW
                     LQVKYGFGPAFIGTITLYTISVLMYWAFFWRRSIDPVPASAAAD"
     gene            510..1670
                     /locus_tag="PKI33_00070"
     CDS             510..1670
                     /locus_tag="PKI33_00070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNM35418.1"
                     /translation="MDKDILIIGGGPSGLSTALHLAKYAPHLTQRILILEKEHYPRLK
                     LCAGGLVIDAERILEHLGLDVTEIPHVDSNSIRFDFAGSGINIRVPKRHALRVIRRDE
                     FDHWLAKQVESKGIEIRQGVTVRKISPDANGVTVETDQETLRATLVVGADGSNGITRR
                     SIFPNEPIYTARVLEVLTPVSPVSSNSTTPRNQTALFDFFPVPDNIAGYVWDFPTQIK
                     GMEMRCWGIYDTNLLASQKRPALKDPLAKEMQRHGFDLNEYEIKGHPIRWYSPGNKAS
                     VSRVLLVGDAVGADVIFGEGISMALGYGNLAAKEIADSFQRGDFSFRRYKRRLERSGL
                     GQTLFARWLIAQIIYNLKWRWFQILLWRIVKPIVLFVAWVFVLNWSQRDLRY"
     gene            1700..2470
                     /locus_tag="PKI33_00075"
     CDS             1700..2470
                     /locus_tag="PKI33_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNM35419.1"
                     /translation="MGTADLHVHSIYSRDATTTVRAILKQASSVGLDIIAVTDHDEIR
                     GSLEAQQLAPQYGIEVISGAEVSTKDGHLVALFIKDLPPAGMSLTDTLLHIGDQGGLA
                     IIPHPFNNLPNSLTLEAVFTAMENPTAKASLKGMETHNFGTQNFDKMARKLSVFLPFS
                     KISSSDAHIYWAIGAGRTEFPGKTTQDLRDAIENNKTVPLLYEGESSAKALLSWARRI
                     TMRRFGYASDTSSATEDINTRQMSDSLIRRIQKKESSS"
     gene            complement(2481..3239)
                     /gene="recO"
                     /locus_tag="PKI33_00080"
     CDS             complement(2481..3239)
                     /gene="recO"
                     /locus_tag="PKI33_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNM35420.1"
                     /translation="MTDFRSFRASAVVLRHADWGEADRILTLYTRDQGIVRALAKGAR
                     KVTSRKAGHLQPFTHVTVQLAKGRDLLIITQVETINAYLPLHDDLIKTGYAAYIIELL
                     LRFSYEDEGANPAIFRLVTETLDRLEKETDAWLPVRYYEMRLLDAVGFRPQLFQCANC
                     SREILPEDQYFSFTAGGVICPRCGEGIPNLAHISLEMLKYLRHFQRSSYKEASKARPS
                     LEVQKEAEVLMQGYFTYLLERELNTPGFLKKVKT"
     gene            complement(3282..3692)
                     /gene="cdd"
                     /locus_tag="PKI33_00085"
     CDS             complement(3282..3692)
                     /gene="cdd"
                     /locus_tag="PKI33_00085"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNM35421.1"
                     /translation="MTISTVEKQSLIDLANEARRRAYVPYSNYPVGAALRTKTGRIYT
                     GVNVENAAYPQTMCAERVAIFKAVSEGETEFEVIAVVTNNGGSPCGGCRQVMAEFGLD
                     AIVLFGNGQGELTHETTVHDLLPEAFTPKHLVTE"
     gene            complement(3705..5069)
                     /gene="glmU"
                     /locus_tag="PKI33_00090"
     CDS             complement(3705..5069)
                     /gene="glmU"
                     /locus_tag="PKI33_00090"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNM35422.1"
                     /translation="MKITAVLLAAGQGTRMKSSLPKVLHPVAGKPMIWHALQAIQQST
                     TEKPVVVVGHGAEEVTNYLSDSAQTVLQEPQLGTGHAVMQAESLLRGKTDLVVVCYSD
                     MPLLRGETLQKLVETQKQNKGPVSLLTVVAENPRGFGRILRKDDGSVTAIVEEYVATP
                     EQLQVKELNVGGYCFDANWLWDALHRIPKNPKKGEYYLTDTIELAVKDGLSVHATVMD
                     DLEETIGVNTRVHLSEVESAMRRRINQTHMLNGVTMIDPSSAYIEAEVTIGKDTVIMP
                     NTYLFGNTEIGERNVIGPNTIIRNSKVGNGCKILASDLEGALLENDVDMGPFARLRKG
                     AHLGNHVHMGNFGEVKDSYLADGVKMGHFSYIGNAKIGSNTNIGAGTITANYDGEKKN
                     PTEIGEDVFIGSDTMLVAPVKLGDGARTGAGAIVTKNVPDDTLVVGMPARAIRKLERK
                     SKKK"
     gene            complement(5162..5235)
                     /locus_tag="PKI33_00095"
     tRNA            complement(5162..5235)
                     /locus_tag="PKI33_00095"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5201..5203),aa:Gln,seq:ttg)
     gene            complement(5265..6350)
                     /locus_tag="PKI33_00100"
     CDS             complement(5265..6350)
                     /locus_tag="PKI33_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM35423.1"
                     /translation="MIRNVLLVDDQRDILRLLRSTLDTLKNKDIQIYEAQSGEEAMLE
                     SSRHKIDLLVTDYKLPGMSGVELMHKVRARHAEVKVIFITGMTDRKMREDMLNSGAFG
                     IFDKPIPIADFLDSVERGLGFVQTIFPPELTAEANALGEGEIQQTKVSDLLANFRQDF
                     NANAVFLLNDRGLINARTGNLRDPSMEVSLISTLMAIHNTSLKVAKVNHQEVVESYHV
                     FSGGDHDLLFIPVTPLYALLVAGKDLATENQILDTVRGLMALRSQVEKALSTLGVTGQ
                     LKFKTAPLAVPPTEAPPKKNTDQLAKATPAPEMEALLSNASGEKAKEKSTDDFWESAA
                     QDLGRKSINPEVISLEEAKKMGLIPGE"
     gene            complement(6457..8382)
                     /locus_tag="PKI33_00105"
     CDS             complement(6457..8382)
                     /locus_tag="PKI33_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNM35424.1"
                     /translation="MKKNVIPFLLVIVLIVIPACLSAPAQTETPAPTESFIEITETPP
                     PSLGPSLTVCLGQEPNSLYPFGDLNDAARNVLSAIYDGPIDNVGYEYQPVILTKLPSI
                     ENGDAQIVPVKINNGDSVVGADGNIIQFSKGSIVRPAGCRNADCAITYDGTSSIEMDQ
                     MIVTFRLRPDLTWSDGTPLTADDSIYSFELQKESKVNTYLTERTQTYEATDEQTLQWF
                     SIPGYLDPTYFLNFWQPAPKHAWSEFTPDQLPTVDVSSREPLGWGPYTVKEWLPGEGL
                     TLEKNPYYYRSRDGYPKLDSIQFRFIPDPDLALSELIAGRCDLIDPTINLEDKVGLLQ
                     EMQTSGQAKFFATTGMSMEWLGLGITPASYDNGYDIQKDRQNFFLDTYPRQAIAYCLD
                     RQTIATSVLHGLTEVPTSYLPLGHPTLDTNLSAINYDPEIGNSLLDQAGWLDEDENPS
                     TPRRAINAQKVAYNTPLILNYQTTSTTQRRQVAAILEQSLANCGIGLNVEFISPNDLY
                     APGPVGPLFGRQFDLAEYALGVEGSEPPCGWFSTSSIPSEANSWSGTNVTGFSNEEYD
                     AACQAGQSALQEDESYLSAYRQTQIILADQLPAIPLFPRLHIAAARPDLCGFDLDPSA
                     RSLWNAEAFEISETCQN"
     gene            complement(8471..9460)
                     /locus_tag="PKI33_00110"
     CDS             complement(8471..9460)
                     /locus_tag="PKI33_00110"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNM35425.1"
                     /translation="MNKINKQEQPELLVVDNLVKYFPVRTGLFQRVTAWVQAVDKVSF
                     AVKKGETLGMVGESGCGKTTVGRTLLRLIEPTSGNVTFEGKDVLKLNQRELKPLRRDM
                     QIIFQDPYASLNPRMPIGESVMEGLQIHNIGHPKERWEIAVNMLKKVGLEEYHARRYP
                     HEFSGGQRQRIGIARALALNPKFIVCDEPVSALDVSIQSQVLNILKDLQEEFGLTYLF
                     IAHNLSVVEHISDRVAVMYLGKMVELTDRDSLYREPLHPYTKALLSAIPVGHPNEKRE
                     RTILKGDVPSPLNPPKGCRFHTRCPIAVSQCSQEEPTFKEVRPGHWVACLLAQ"
     gene            complement(9475..>9881)
                     /locus_tag="PKI33_00115"
     CDS             complement(9475..>9881)
                     /locus_tag="PKI33_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HNM35426.1"
                     /translation="LGSALMLITHDLGVIAEMANRVVVMYAGEIVEESPVASLFDKPH
                     HPYTQGLIGSIPVLGQRRDRLDVIPGNVPNLVNLPQGCRFAPRCRARTEHNLSICTEK
                     HPGLIQVADGHKVRCWLYEDADGHTAPLKSGK"
BASE COUNT         2598 a         2393 c         2291 g         2599 t
ORIGIN      
        1 gttcactggc aatgggcatt ggtctgctta tcgcgccacc tctcgcagac cgaacaggca
       61 aaatccaact cgtggtaatt acacaagcgc tttccattcc gttccttatt ttgctaggtt
      121 tctctcccat cttttgggtt ggcgctgtca gttattacat ccgccttgca ctcatgaaca
      181 tgagcagccc ggtctaccaa accttcgtga tggaacatgt tgagccttca gggcgagcca
      241 cggttgcaag cctcaccagc atggcatgga acttcggctg ggcgttcagc cctaccatca
      301 gtggatggtt gcaagtgaag tatgggtttg gtccggcgtt cattggaacc atcacacttt
      361 atacgatctc ggtcctcatg tattgggcat tcttctggcg ccgaagcatt gacccggtcc
      421 cagcttcggc agccgcagat tagaaaagtc tcaccgattt tggaaaaaca gagcattgaa
      481 attgctctgt ttttttataa aatatcttaa tggacaaaga tatcttgatt attggcggcg
      541 gtccgagcgg actctcgact gcccttcatc tcgcaaaata tgccccgcat ctgactcagc
      601 gtattttgat cctcgaaaag gaacattatc cacgcctgaa gctttgtgct ggcggcttgg
      661 tcatcgatgc tgaacggatt ctcgagcatc tcggcttgga cgttaccgaa atcccgcatg
      721 tggattcaaa ctcgatccgc tttgatttcg caggcagcgg tattaacatc cgtgtaccga
      781 aacgccacgc cctgcgcgtg attcgccgcg acgagttcga tcactggttg gcaaagcaag
      841 ttgaaagcaa gggcatcgag attcgccaag gtgtgacggt taggaaaatc tctccagacg
      901 ccaatggcgt caccgttgag accgatcaag agaccctacg tgctaccctc gtcgttggcg
      961 cagatggctc gaatggcatt acacgccgca gcattttccc aaacgagccg atttatacag
     1021 cccgcgtact ggaagtacta acgccggttt cgccggtttc aagtaactcc acaacaccca
     1081 gaaaccagac agctttgttt gacttttttc cagtccctga taatattgcg gggtacgttt
     1141 gggattttcc aacccaaatt aaaggtatgg aaatgcgttg ctggggcatc tacgatacaa
     1201 atctgcttgc aagccagaaa cgcccggcac ttaaagaccc gcttgcaaaa gaaatgcagc
     1261 gacatggttt tgatctgaat gagtatgaaa tcaaggggca ccccattcgt tggtacagcc
     1321 cggggaataa ggcttcggtt tcaagggttt tactggtagg agatgcagta ggtgccgatg
     1381 tgatctttgg cgaaggcatt agtatggcat tagggtatgg gaacctcgcc gccaaagaga
     1441 tcgcagattc gttccagcgt ggtgactttt cgtttcgcag gtacaagcgt cggctggaaa
     1501 gaagcgggtt gggtcaaacg ttatttgcac gttggctcat cgcgcaaatt atttataacc
     1561 tcaaatggcg ctggttccaa atcctgcttt ggcgcatcgt gaaaccaatc gtcctttttg
     1621 tggcatgggt ttttgtacta aattggagtc aaagagacct ccgatattga acatataaaa
     1681 gaaaagaaag taacagcaca tgggcaccgc agaccttcac gttcactcca tctactcccg
     1741 agacgcaacc accactgtgc gagccatcct caagcaagct tcaagtgtcg ggctcgacat
     1801 tattgcagtg accgatcacg atgaaataag gggctctctc gaagcacaac aactggcacc
     1861 acaatatgga atcgaagtta tttctggggc tgaagttagc acaaaggacg ggcacttggt
     1921 ggcattgttc atcaaggacc ttccaccggc tggcatgtct ctaaccgata ccttattgca
     1981 tattggagat cagggtgggc ttgccatcat ccctcaccca ttcaataatc ttccaaacag
     2041 ccttacgttg gaagccgtat tcactgccat ggaaaacccc acagcaaagg catcgcttaa
     2101 aggaatggaa acccataact ttggcacaca gaactttgac aaaatggcgc gtaaactttc
     2161 tgtgttcctt ccattctcaa aaatatcttc aagcgatgcc catatctatt gggctattgg
     2221 cgccgggcgc acagagttcc ctggtaaaac cacgcaagat ctgcgtgatg caatagagaa
     2281 taacaaaact gtgccactgc tctatgaagg cgaatcatct gccaaggcgc ttttgagttg
     2341 ggcgcgccgc attaccatgc gcaggtttgg gtatgcttct gatacgagtt ctgcgacaga
     2401 agatataaat acaaggcaaa tgtcagattc tcttatccgt agaattcaaa aaaaagagag
     2461 cagttcgtaa ctgctctctt ttatgttttg accttcttta ggaagccggg ggtatttaat
     2521 tctcgttcca acaaataagt gaaatagcct tgcataagaa cttccgcctc tttctggacc
     2581 tcaagactgg gtcttgcttt tgaggcttct ttgtagctcg aacgttggaa atgccgcaaa
     2641 tatttgagca tttccaatga aatatgtgcc aggttcggaa tcccctcccc acaacggggg
     2701 cagatcaccc ctcccgccgt aaacgagaaa tattgatctt ctggcaaaat ttcgcgcgaa
     2761 caattagcgc attggaataa ttgcgggcga aaccctacag catccaataa cctcatctca
     2821 taatagcgga cgggtaacca agcatctgtc tctttttcaa ggcgatccaa agtttcagtc
     2881 acaagccgga aaattgccgg gttcgcgcct tcatcttcat atgaaaagcg taaaagcaat
     2941 tcaataatgt atgcggcgta accagttttt atcaagtcat catgcaaggg taaataagca
     3001 ttaatcgttt caacttgggt aataatcagt aagtctcgcc ctttagcaag ctgaactgtg
     3061 acatgggtaa aaggttgtaa atgcccggct ttccgtgatg tgacttttct ggcacctttt
     3121 gctaacgcac gcactatccc ttggtcacga gtgtaaagcg tgagaatacg gtcagcttcg
     3181 ccccagtccg catggcgaag cacaactgca gaagcacgaa acgaacgaaa gtcggtcata
     3241 aaagaacatg gagaatggtt ttgtccattc tccacaagtt tctattcagt cactaaatgt
     3301 tttggggtaa aggcttcagg taaaaggtca tgaactgtag tttcatgtgt caactcacct
     3361 tgcccgttgc cgaacaatac aatagcatcc aagccgaact ctgccattac ttgcctgcat
     3421 ccgccgcaag gagaaccgcc gttattggta acaactgcga tcacttcaaa ttctgtctca
     3481 ccttctgaaa cggctttgaa aatcgccacg cgctccgcac acatggtctg tggataggca
     3541 gcattctcca cattcacacc ggtgtaaatg cgcccagtct tggtccgcaa ggctgcgcct
     3601 acggggtaat tcgagtaagg aacatatgca cgacggcgtg cttcatttgc aagatcgatc
     3661 aaagattgtt tttctacagt tgagatcgtc attctaaacc tgttctattt cttttttgat
     3721 ttccgttcca gtttacgaat cgcacgagct ggcatcccca ctaccagggt atcgtctgga
     3781 acattcttcg ttacaatcgc gcccgcgcct gtgcgcgcgc catccccaag tttaaccggc
     3841 gccaccaaca tggtatctga cccgataaat acatcttcac cgatctctgt tgggtttttc
     3901 ttctcgccat cgtaattggc agtgatcgtc cctgcgccga tgttggtatt cgaaccgatc
     3961 ttcgcattcc caatatacga gaagtgaccc attttcacac catcagcaag ataagaatct
     4021 ttcacttcgc caaaattccc catatgaacg tgattcccca aatgtgcacc tttgcgtaaa
     4081 cgggcaaagg gtcccatatc cacatcattt tcaagaaggg caccttcaag gtctgaagct
     4141 aaaattttgc agccattccc aactttagaa ttgcgaatta tcgtgtttgg tccaatcaca
     4201 ttccgttcgc caatttctgt gtttccaaac aaataggtat tcggcataat cactgtatct
     4261 ttgccaatcg tcacttcagc ttcgatataa gcagatgatg ggtcaatcat tgtcacacca
     4321 tttaacatat gcgtctgatt gatacgtcta cgcatggcac tttccacttc agaaagatgc
     4381 acacgagtat tcacaccaat cgtctcttcg aggtcatcca tcactgtagc atgaacagac
     4441 aaaccatctt tgactgcaag ttcaatggta tccgttagat aatattcgcc ctttttggga
     4501 ttcttaggga tacgatgtaa agcatcccac agccaattgg catcaaaaca ataaccaccc
     4561 acgttcaatt ctttgacctg taactgttca ggcgttgcga catattcttc cacgatggca
     4621 gtcaccgatc catcatcctt acgaaggatg cgaccaaatc cgcgtgggtt ttctgcaact
     4681 actgttaata aagaaaccgg tcctttattc tgcttctgcg tttcaactag tttctgtaga
     4741 gtctctccgc gaagtaaagg catgtcggag tagcaaacaa ctacgaggtc cgttttgccg
     4801 cgcaagagag attcggcttg catcacagca tgacctgttc ccagttgcgg ttcctgtaag
     4861 acagtttgtg ccgagtcgct aagataattg gttacttctt ctgcaccatg ccccaccacc
     4921 acaacgggtt tctctgtggt cgattgctgg atcgcctgta aggcatgcca gatcataggc
     4981 ttaccagcta cagggtgcaa cactttcggc aatgaggatt tcatgcgtgt gccctgccca
     5041 gcggcaagaa gaacagcggt tatcttcatg gattcacctc agataaaaac aggatttgtg
     5101 cggttgacgt gctccgcgac aaatcccgtt tgagaaaatt gtccgcttgc gcggacatgt
     5161 ctggctgggg cacctggatt cgaaccaaga tccacagctc caaaggctgg tgtgctgcca
     5221 ttgcaccatg ccccagtgcg ggcaagattg tatcacaatt ttttttattc gccgggtata
     5281 agtcccattt ttttagcttc ttccagagag atcacttcag ggttaataga ctttctaccc
     5341 aagtcttgcg ccgcagactc ccaaaaatca tctgtcgact tttccttggc tttctcgcca
     5401 gaagcattac tcagcaacgc ctccatttct ggagcggggg ttgctttggc taactggtca
     5461 gtattcttct ttggaggtgc ttctgtaggc ggaactgcta gtggtgcagt tttaaacttt
     5521 aattgtccgg taacccctaa tgtggataat gctttctcta cctgagaacg caaagccatc
     5581 aagccacgca cggtatccag aatttgattt tcagttgcca agtcctttcc agcgacaagc
     5641 aacgcataaa gcggagtcac tgggataaac agcaaatcat ggtctccccc actaaagaca
     5701 tgataggact caaccacttc ttgatgatta acctttgcca ctttcaaact ggtattatga
     5761 atcgccatca aagtggaaat caatgagact tccatactgg ggtcacgaag gttcccggtg
     5821 cgggcattaa tcaaaccgcg atcgttaagc agaaatacag cattggcatt gaaatcctgc
     5881 ctgaaattgg caagcaagtc agaaactttc gtctgttgta tttccccttc gccaagtgca
     5941 ttcgcttcag cggtaagctc cggtggaaag atggtttgta caaaaccaag tccacgttca
     6001 acagagtcta gaaaatcggc aattgggata ggtttatcaa agatgccaaa agcaccactg
     6061 ttgagcatat cttcgcgcat tttgcggtcg gtcatgccgg taatgaaaat aacttttact
     6121 tctgcatgac gagcacgaac tttatgcatt aactccacgc cactcatgcc gggaagtttg
     6181 taatcagtca ctaaaagatc tattttatga cgggaagact ccaacatagc ctcttcccct
     6241 gattgggctt cgtagatctg aatatcctta ttcttgagcg tgtccaaggt ggaacgcaaa
     6301 agtcgtagaa tatcgcgttg gtcgtccact aacaaaacgt ttcgaatcat accagcctgt
     6361 tgtttgaaat caatggaata aattcgtttg gattaataac atccaaactt gataacgatg
     6421 tctaaattat agcaagattc aggaattgct tcccaatcag ttttgacagg tttcgctgat
     6481 ctcaaatgcc tcggcattcc aaagcgaccg ggcagatggg tcaaggtcga aaccgcaaag
     6541 gtcggggcga gcagccgcta tgtgcaagcg agggaaaagc gggatggctg gcaactgatc
     6601 cgccaaaatg atctgagtct ggcgatatgc tgatagataa gattcatctt cctgtaaagc
     6661 ggattgcccc gcctgacagg cggcatcgta ttcctcatta ctaaagcctg ttacatttgt
     6721 accagaccat gagttcgcct cacttggaat tgatgacgta ctgaaccaac cacatggtgg
     6781 ttcgctaccc tctacaccga gagcatattc tgctaggtca aattggcgac caaataaggg
     6841 tccaaccgga ccgggagcat ataggtcgtt gggcgatatg aattcgacgt tcaatccaat
     6901 tccacagttt gcaagagatt gctccaaaat ggcggctact tgcctgcgtt gggtagtgga
     6961 ggtggtttga tagttcaaaa taagcggggt gttataagcc actttttggg cattgatcgc
     7021 ccggcgtggc gtggaagggt tttcatcttc atccaaccag cctgcttgat ctaacaaaga
     7081 atttccaatt tcggggtcat agttaatagc agagagattt gtatccaacg tggggtgacc
     7141 caacggtaaa taactggttg gaacttccgt cagcccatga agaacggacg tggctatagt
     7201 ttggcggtct aaacagtaag caatggcttg gcggggatat gtatccaaga aaaagttttg
     7261 gcgatctttc tgaatatcat agccgttatc ataagaagca ggtgtaattc ccaagccaag
     7321 ccattccatg ctcatgccag tggttgcaaa aaactttgcc tgaccagaag tctgcatttc
     7381 ttgcagcaat ccaactttgt cttcaaggtt aatggtaggg tcgatcaagt cacagcgacc
     7441 tgcaattaat tcggacagag ccagatctgg gtcgggaatg aagcggaatt gaatgctatc
     7501 caatttagga tacccatcgc gagaccggta atagtaaggg ttcttttcga gcgtgagtcc
     7561 ttcgcctggc aaccattctt tgacggtgta aggaccccat cctaatggct cacgtgaaga
     7621 gacatcaaca gtgggcaatt ggtcaggcgt gaattcgctc caagcatgtt tcggagcagg
     7681 ctgccaaaaa ttcaaaaagt aggtcgggtc gagatacccc ggaatactga accattgcag
     7741 ggtttgctca tcggtggctt cataggtttg agtgcgctca gtgagatatg tattgacttt
     7801 gctttctttt tgcagttcaa acgaatagat ggaatcatca gctgtcagcg gagtgccgtc
     7861 agaccaagtc aggtcggggc gtaggcgaaa tgtcactatc atttggtcca tttcgataga
     7921 agaggtacca tcgtatgtga tggcacagtc agcattacgg cagccagcag ggcgcacaat
     7981 ggagccttta ctgaactgaa taatatttcc atcggcacca acaacagagt ccccattatt
     8041 tatctttaca ggaacaatct gcgcatcgcc attttcaata gaaggaagtt tggtgagaat
     8101 cactggctga tattcatagc ccacattatc aatcggtccg tcatagatcg cagagagaac
     8161 attacgagcc gcatcattca aatctccgaa tggatacaaa ctattcggct cttgccccaa
     8221 acagacggta agtgagggtc ccaaagaagg cgggggtgtt tcagtaattt ctataaatga
     8281 ttcagtcggt gccggggttt ctgtctgtgc aggcgcactt aaacaggctg gaatcacaat
     8341 aagaacaata acaagtaaaa atgggatgac gttttttttc atagctcctg ccatatctgt
     8401 ttaaggcgtt tcgtaattat aaccactccc ctattataaa aagagaccgc gtgtgcggtc
     8461 tctttatttt ttattgggct aacaaacagg ccacccaatg acctgggcga acttccttaa
     8521 aggttggctc ttcctgcgaa cactgactga ctgcaattgg gcagcgtgta tggaagcgac
     8581 aaccctttgg cgggttcagc gggctgggca catctccttt gagaatagtg cgctcccgtt
     8641 tctcattcgg gtgccccaca gggatggcag aaagcaatgc cttggtgtaa ggatgaagcg
     8701 gttcacgata caacgagtca cggtcagtca actcgaccat tttgccaaga tacatcaccg
     8761 ctacacggtc tgagatatgt tccaccacac taagattatg ggcaatgaaa agatatgtga
     8821 gaccgaactc ttcttgaagg tcttttagga tgttaagcac ctgagattga atggaaacat
     8881 ccaaagcgga aaccggctca tcacaaacga tgaacttggg attcaatgcc aaagcacggg
     8941 ctatgccgat acgttgacgc tgaccgcccg aaaattcgtg cggatagcgg cgggcatggt
     9001 attcttccaa gcccactttt ttgagcatat tgacagcgat ctcccagcgt tctttcgggt
     9061 gaccaatgtt gtgaatttgt aaaccttcca tgaccgactc gccgataggc atacgtggat
     9121 taagagatgc atatgggtct tgaaagatga tttgcatatc acggcgtaaa ggttttagtt
     9181 cacgctgatt tagttttaag acatcctttc cttcgaatgt cacattgcca gaggttggtt
     9241 cgatcaggcg caaaagggtg cgtcctacgg ttgttttgcc acaaccagac tcacccacca
     9301 tgccgagggt ttcgcctttt ttcacggcaa agctgacttt gtccaccgct tgaacccaag
     9361 cggttacgcg ctgaaataaa cctgtacgaa caggaaagta cttgacgagg ttatcaacca
     9421 ccaacagttc tggctgttct tgtttgttta tcttattcat ctaagccgcg tcctttattt
     9481 accagatttc agtggtgcag tgtgtccatc cgcatcttca tacaaccagc aacgaacttt
     9541 atgcccatct gctacttgga tcaagccagg atgtttctcg gtacaaatgg aaaggttatg
     9601 ttctgtgcga gcgcgacaac gaggagcaaa gcggcagcct tgcggaagat tgacgaggtt
     9661 cggaacattt ccggggatga catccaaacg gtcacggcgt tgacccaaaa ctgggatgga
     9721 accgatcaag ccttgggtgt aagggtgatg cggtttgtca aaaagagaag caaccggaga
     9781 ttcttctacg atctcgcctg catacatcac aacaacacga ttcgccattt cagcgatcac
     9841 gccgaggtca tgtgtaatga gcatcaaagc agagccgagt t
//
LOCUS       DAOREF010000003         9238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_3532_length_9238_cov_5.093542, whole genome shotgun sequence.
ACCESSION   DAOREF010000003 DAOREF010000000
VERSION     DAOREF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_9238_cov_5.093542"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKI33_00120"
     CDS             <1..684
                     /locus_tag="PKI33_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM35427.1"
                     /translation="WMGDYLNQQGYTCLGIRLAGHATTPEDMIRARWTDWTASVEDGY
                     NLLRGATDKIFLVGLSMGGVLSLLMSTRLDVAGVVAMSTPYQLPRDEKDYSPEFIEFY
                     SRFVKFMPKSTEVPGASWFDKEAFQSHISYPQNPIRSIAEMKRLILVMRAALPNVTKP
                     VLLIHSKNDKYVLPENVERLYDGLVNASDKTKAYVVEAGHVVTRDAARHQVFEMARDF
                     IRRVDASHS"
     gene            705..1832
                     /locus_tag="PKI33_00125"
     CDS             705..1832
                     /locus_tag="PKI33_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM35428.1"
                     /translation="MNRNLSFIAAALLLWGFGEGMFFNFVPIYLDVQFLLSKYEIGLI
                     LGGFGFFMAITHIPAGRLADRLGRKPLLIVAWLLGLLSTLTMGSAKTLPFFLIGLFTY
                     GLTAFVSSPLSSYVTAARGQLPVATVLSLTTATFGMGMVLGPVTSGWIGEHYGMRMSF
                     YAAAIVFVFSNIFVYFIGSQPIDHHDPSAPPLGLGSNKKFVTLMFVLAFAIFSMYLAQ
                     PLTPNFLEGVRGLSLSQTGLIFTIGALGNSLMAITFSRVNPRYGFLAAQSLVILFAVL
                     IWYGTSLPFFALGYFLLGGFRAGRPMALAQARELVHESQMGVTYGVMETVSAIITIIT
                     PPIAGFLFEFDPFIVYPISIGLLVLSILLSSVFLPRKESHA"
     gene            1825..2460
                     /locus_tag="PKI33_00130"
     CDS             1825..2460
                     /locus_tag="PKI33_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM35429.1"
                     /translation="MLEIVQFTLGPASTNAYLVADPETKEAVVIDPSWDGHLILAEAQ
                     KRGWRIGHLWYTHAHFDHIGGAAAIADAMNPLPHVALHPDDHVLWREGGGGKKFGLDI
                     DPGPEPTIDLIHGMTMKLGSNDFQVRFTPGHTRGHCVLYVAKENICFCGDLIFNESVG
                     RTDLPGSSFSALEKSIREQIYTMPDETVLLSGHGPKTTVGHEKKNNPFVSE"
     gene            2479..3369
                     /locus_tag="PKI33_00135"
     CDS             2479..3369
                     /locus_tag="PKI33_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35430.1"
                     /translation="MKRAVGISLGSSKRDKKVIVNLNGVEIQVERIGTDGDIEKARRL
                     YLELDGKVDAFGVGGVDLYLRLDAREYPLHAALKLVSGVKQTPLCDGRGLKHTLERRV
                     FQLAKAELGEVRFKQAFIPVAVDRMGLAEAVAEVSDQIVSGDLMVALGVPIPLYGIPA
                     FKRVASVMLPMVSYFPMSMIFYGSDGAEQEPKYVKYFEESDLIAGDFLFMRKYMPKSL
                     AGKTVVTNTTTEENIALLKERGVKTVITTTPRYDGRSFGTNTTEAMLTAYADKGRRLT
                     DEELNGLIDELGLKPSVISL"
     gene            3485..5428
                     /locus_tag="PKI33_00140"
     CDS             3485..5428
                     /locus_tag="PKI33_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM35431.1"
                     /translation="MTKKQLRVFLCSGKEAQEHVRLLHRRLCADGIDCILDEEIPIGG
                     QNWKVEIRKKIKSSDIVIICLLKKAIAKEGFIHEEITLALEADKEKPDGTIYLIPAKF
                     ERCSLPAPMKEKQAVDLFTAKGYEKLTNALKVRANELKRAFPEHVSKGPLFEFDGQIE
                     FSKENTITAGSNFSSVMFVNRVDEIEAVTATASSGGQYFLVDAPAGYGKTWFMHKLDE
                     YFAVKKNWLHAMVSVKKDCNLEVLAKELAKKLGLELRSGQGSDWGFRLSGAIKKRYST
                     DTMPAGLVLLIDLDGDPSFLLAQDILEKFIPQIWEILSDLDAFSRSATPSLFRVILSG
                     RNITTEKSIKIYRDQLPFINKILSPFTYDVVYKSAKNHLPQIEKKKLEQLVAHLLFLT
                     GGHPGCMSSVLDLYKSGDIPIERFVTDTQQAIWDQIVRDVTEKVWTGLNYRDKKLQEA
                     IHRLSVYRYVNNHVLRYVIENCPVKGMRDEIDLRDMLTGSYLYTNDGRFLQDGIIRQL
                     VLIHLRQTMPKLYEGYCQDAYQVCKDQLTSPNVQLPEKWIVEMLNQCLLQHAGNGIEQ
                     PAFRSKISKQFNKEVMSIMQVRKKTKSSLSDWREEVDALLSEMDKEPQWEFRFSVNYF
                     LRDGQYTDEPFQKLKAEIKKQLK"
     gene            5439..8126
                     /locus_tag="PKI33_00145"
     CDS             5439..8126
                     /locus_tag="PKI33_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM35432.1"
                     /translation="MANKIPFVDRKKELDLIEKHIVKSKGKHILWIQGPGGSGKTRLL
                     EEVLLRYGYLKKNGYEFSPIVDFDDRSLHIPENLDRMLVQWLGEKPLKQYLRETLDRQ
                     KMEEAGVSEETLLRQSDHVTQWLIETFNQSSKKKRRILLFDTVEKVEDKVWQHICDLL
                     QHAENTFILLVSRKNDRLLDTLNSKITKSIEIIQWEPLEDEKFGELYLDQKQRSLHVA
                     LPPSICKKLVLLVGGRPILIDLTVEAISRNLMPEILTHKTYAEIKALSSRKSGKLRQD
                     FETQLVRQIMDVRDSMDRLVLVMSRVYPLSQKMVTEFLNITERDAKNLFEEAKLKTTI
                     KILPDGRISLHDEIRTMVENYVWPYIDPDGDRQKRDSRIAIDYLRHEEVEIQKDLKKL
                     EDQASVEKTQDAGISLDVMSKMESLKQYLWDVREQLLAHSLYINTEEGIQTFADIFDR
                     ATRAYHFPYREVLLRKVEPYMPHLPVALKYEVNSRKVKASLDTGDYIEADRLTTQVLK
                     DKSIAPQQRVDMLIQHGNIKIRLGKLSEGKEKFEKAVRICKAGNDIRNLVPALNARGW
                     GYRQVGNIDKAIIDYQNALELSFSTGDELRRAWILNNLAYVYSQQGRFNPALALCEQA
                     KELWKKIGFGRGMGALYEVYGHIYIKMGRYDEAMEYFQLALDEFKSRDVEWQSRIHAG
                     LGFVYRMMKNRKNAERELKLALDYRIQKDRAMILHRMAHLQLEGGKIKKAKNLFEQSY
                     EASREISDAYHELNNLGDLAKLAVGEKDFSNAARFEKLYQEYKTTWPDMTYSRVEGMI
                     LRHLGDLMLGNPKNNLSKAITYYQKAFLLLARYETYKEYSVQKQLEILDAYFHELKVP
                     ANVKSKIGQSLYFVWQKNNLIEHHPEALGFFAQWGGTVK"
     gene            8236..>9238
                     /locus_tag="PKI33_00150"
     CDS             8236..>9238
                     /locus_tag="PKI33_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNM35433.1"
                     /translation="MAEKGNMFLANEYYPAYITASLELDKGMHLVCTADSSRPALLND
                     QGWELFSRIVNQQPITGSLAGTACGSFGQSLIAAGLLLPGSRNATPKEQNHILTSWLH
                     LTDRCNLRCSYCYLPHVLEDMSLKTGYVAMDAIFRSALAHGFKQVKIKYAGGEPLLCF
                     ATLITLHTYAQQLAEKHRLDLDGVILSNGTLLTVEMVEMLKTFDLRLMISLDGLGNYH
                     DSHRPYAGGRGTFADVSEAIDLALAHGVVPDISVTVSNRTADGLANVIAWILDRNLPF
                     SLNFYRENELSVSNTDMRLDDERIVRGVLAAYRVIEDRIPQRSLLAALIDRANLAVPH
                     "
BASE COUNT         2686 a         1936 c         2162 g         2454 t
ORIGIN      
        1 tggatggggg attatctcaa tcaacaagga tatacctgct taggcatccg actcgcgggg
       61 catgcgacaa cacccgaaga tatgatccgc gcacgttgga cggattggac ggcttctgtg
      121 gaagacgggt acaacttgct gcgtggtgca acagacaaaa tctttttagt ggggctttct
      181 atgggcggcg tgttgtcgtt gttaatgtca actcgtttgg atgtggcggg tgtagtggca
      241 atgtccacgc cgtatcaact gccgcgcgat gaaaaagatt attcgccgga attcattgag
      301 ttttacagtc gcttcgtaaa gttcatgccg aaatcaaccg aagtgccggg cgcttcctgg
      361 ttcgataaag aagcattcca gagccatatt tcgtatccac aaaatcccat tcgttcgatc
      421 gcagaaatga aacggcttat ccttgtgatg cgggcggcat tgcccaatgt gaccaagccc
      481 gtattgctta ttcactcaaa gaatgacaag tatgttctgc ccgaaaatgt ggagcggttg
      541 tacgatggac tggtcaatgc atcagacaag acaaaagcct atgtggtcga ggcaggtcac
      601 gttgtgacaa gggatgcggc tcgtcatcaa gtctttgaaa tggcgcggga ttttatccga
      661 cgagtagatg cgtctcactc gtaagatttt caaaagagga atcgttgaat cgtaatcttt
      721 ctttcattgc tgcggcattg ctgttatggg gatttggcga aggcatgttc ttcaattttg
      781 tgccgatcta tttggatgtg caatttttgt taagtaaata cgaaattggg ctcattcttg
      841 gcgggttcgg ctttttcatg gcgatcacgc acatcccggc tggaaggttg gcagaccgcc
      901 tgggtagaaa acctttgctt attgttgctt ggctgttggg tctactttca actcttacaa
      961 tgggaagcgc aaagacgctg ccctttttct taatcggttt atttacatat ggtctgacag
     1021 cttttgtgtc atcaccatta agcagctatg taaccgctgc acgcggacag ttgcctgttg
     1081 caaccgtgct ttcactcacc acagctacat tcggaatggg catggttcta ggacccgtaa
     1141 ccagtggttg gatcggcgaa cactatggaa tgcgtatgag cttttatgcg gctgccattg
     1201 tgtttgtatt ttccaacatt ttcgtgtatt ttattggaag ccagcccata gaccatcatg
     1261 acccatctgc tcctccgcta ggtttgggca gcaataaaaa atttgtcacc ttgatgttcg
     1321 ttctggcatt tgcgattttc tccatgtacc ttgcacaacc acttacgcct aattttctag
     1381 aaggcgtgcg cggactttca ctcagccaga cgggtcttat ttttaccatt ggcgcacttg
     1441 gtaattcatt aatggcgatc acgttcagcc gtgtgaatcc gcggtacgga tttttagcag
     1501 cacagtcgtt ggtcattcta tttgcggttc tgatctggta tggaacttct cttccgttct
     1561 ttgcgttagg gtatttcttg ttgggtggtt ttcgagcggg gcgccccatg gcattggcac
     1621 aagcgcgcga gcttgtccat gaatcacaga tgggtgtcac ttatggcgtg atggaaactg
     1681 tcagcgcgat catcacaatc attacaccgc caattgcagg ctttttattc gaatttgacc
     1741 ctttcatcgt ttaccccatc tccattgggt tattggtctt gtctattctt ctaagctctg
     1801 tatttctacc tcgaaaggaa tcccatgctt gagatcgttc aattcactct tggtcccgca
     1861 tctacaaatg cctatcttgt agcagacccc gaaaccaaag aagccgtggt catagacccc
     1921 tcttgggatg gacatttgat cctcgccgaa gcacaaaaac gcggatggcg tattgggcat
     1981 ctttggtaca cacatgccca cttcgatcat attggcgggg cagcagctat tgccgatgca
     2041 atgaatccgc tcccacatgt ggcgcttcac cccgatgacc atgtcctctg gcgcgagggt
     2101 ggcggcggca aaaaattcgg tttggatata gatccgggtc ccgaaccgac cattgacctg
     2161 atccacggca tgaccatgaa actcggctcg aacgacttcc aagtccgctt cacacccggt
     2221 cacacccgcg gacattgtgt attgtatgta gcaaaggaaa atatctgctt ctgtggcgat
     2281 ttgattttca acgaaagcgt cggtcgcacc gatcttccgg ggagcagttt ttcagcgctt
     2341 gagaaaagta tccgcgaaca aatctacacc atgcccgatg aaactgtctt attatcgggt
     2401 catggtccga aaaccacagt agggcatgaa aagaaaaata atccgtttgt gagtgaataa
     2461 aaaaagaaag agtgtgccat gaaacgtgca gtgggtatca gccttggaag ttcaaagcgc
     2521 gataaaaaag tcatcgtcaa cctcaacggc gtggaaatcc aagtcgagcg aattggcacc
     2581 gatggcgata ttgaaaaagc ccgcagactc tatctcgaat tggatggaaa agtggatgcc
     2641 tttggtgttg gtggtgtgga cctttaccta cgtttggatg caagagaata cccgcttcac
     2701 gccgcgctca agcttgtctc tggcgtcaaa cagactccac tctgtgacgg gcgcggactc
     2761 aaacacaccc ttgaacgccg agtcttccaa ttagccaaag ccgaattagg cgaagtgcgc
     2821 ttcaagcaag ctttcatccc tgtggcagta gaccgtatgg ggttggcaga agccgtcgct
     2881 gaagtttccg accagatcgt ctctggcgat ttgatggtgg cattgggtgt gccaatccct
     2941 ttgtatggca tccctgcttt caagcgagtc gcaagtgtaa tgctccccat ggtcagctac
     3001 ttccccatgt ccatgatctt ttatggcagt gatggcgccg aacaagagcc gaagtatgtc
     3061 aaatactttg aagaatctga cttaatcgcc ggtgatttcc tctttatgcg caaatacatg
     3121 cccaaaagct tggcaggtaa aactgtcgtg acaaatacaa caaccgagga aaatatcgcc
     3181 ctcctcaaag aaagaggcgt caaaactgtc atcaccacta ccccacgtta cgacggtcgc
     3241 tcctttggca caaacaccac tgaagccatg ctcactgcct atgcggacaa gggacgcagg
     3301 ctaacggacg aggaattgaa cgggctgatt gatgagttag ggctgaagcc aagcgtgatt
     3361 tctttataac aaggagcaat gtcacctgta ttttgattga ctttgaatat tgcattgcct
     3421 ataatgatta agcatatata accttttctg ctgtggaaaa ggacgctcgt catggtgagg
     3481 gattatgaca aaaaagcagt tgcgtgtctt tttatgctcc ggaaaggaag ctcaggagca
     3541 tgtgcgttta ttacatcgcc gactgtgcgc cgacggaatt gactgtatct tagacgaaga
     3601 aatacctatt gggggacaaa attggaaagt agaaatccga aaaaagataa aaagctcaga
     3661 cattgtcata atttgtttgc tgaagaaagc aattgctaaa gaggggttta tacacgagga
     3721 aattactttg gcattggagg cagacaagga aaagccagat ggaaccattt acttaatccc
     3781 tgcaaagttc gaaagatgtt cattgccagc accgatgaaa gagaaacaag ctgtcgattt
     3841 atttacggct aaggggtatg aaaaacttac caatgctctg aaagtacgtg caaatgaatt
     3901 gaaacgagcc tttcctgaac acgtctcgaa aggaccatta ttcgagtttg atgggcagat
     3961 tgaattttcc aaagagaaca caatcacggc agggtctaat ttttcctcgg taatgttcgt
     4021 aaatcgagtc gatgaaattg aagctgttac agcaacagcc tcatcgggtg ggcaatattt
     4081 tcttgttgat gctccagcag gatatggcaa aacatggttc atgcataagt tagatgaata
     4141 ctttgccgtc aagaagaatt ggttacatgc aatggtgtct gttaagaagg attgcaatct
     4201 tgaagtatta gccaaagaat tagcaaaaaa actaggattg gaattaagat caggacaagg
     4261 ctctgattgg gggtttcgtt taagtggtgc gatcaaaaaa cgctattcca cagatactat
     4321 gccagctggt ctggtattac taattgacct ggatggagat ccatcttttt tattagcaca
     4381 ggatatcttg gaaaaattta tccctcaaat ttgggagatt ttatctgacc tggatgcctt
     4441 ttcgcgttct gcaactccaa gcttgtttcg ggttattctt tctggcagga atattacaac
     4501 cgaaaaaagc ataaagatat accgcgatca acttccattt atcaacaaga ttctttcccc
     4561 atttacatat gatgtggtgt acaagtctgc aaaaaaccat ttaccacaga tagaaaagaa
     4621 gaaattagag caacttgtag ctcatctctt gttcctcacg ggagggcacc caggatgtat
     4681 gagttctgtg ctagatcttt ataaatcggg tgatattccc atcgagcgat ttgtgactga
     4741 tactcagcaa gcaatatggg atcaaattgt tcgcgatgta actgaaaagg tttggacagg
     4801 gctaaactac cgagataaaa aactgcaaga ggcaatccat cgcttaagtg tgtatcggta
     4861 tgtaaacaac cacgttttac gatatgtaat tgaaaactgt cccgtaaaag gaatgcgcga
     4921 tgaaattgat ctgcgtgata tgctaacagg cagttattta tatacaaacg atggacgttt
     4981 tttgcaagat ggcatcatac ggcaactagt cttgatccat ctgcgccaaa caatgccgaa
     5041 actatacgag gggtactgcc aagatgcgta tcaagtttgc aaggatcaat tgacttctcc
     5101 taacgttcaa cttcctgaaa agtggatcgt tgagatgcta aatcaatgtc tattacaaca
     5161 tgcagggaac ggaattgaac agcctgcctt tcgatcaaag atatccaagc aatttaataa
     5221 agaagttatg tcgataatgc aggttcgtaa aaaaacaaaa tcatcgcttt cagattggcg
     5281 agaagaggtc gacgccttgt tatctgaaat ggacaaagaa cctcaatggg aatttcgctt
     5341 ctcggttaac tatttcttgc gcgatgggca gtacactgat gaacccttcc aaaagttgaa
     5401 ggctgagatt aagaagcagt tgaaatagga ggtgtgtcat ggcaaataaa attcctttcg
     5461 ttgatcgaaa aaaagaactt gatcttattg aaaagcacat cgtgaaatcg aagggaaaac
     5521 atattttatg gatccaggga cctgggggta gcggtaagac acgtttgctg gaagaagtgc
     5581 tgttacgcta tggatactta aaaaagaacg gatatgaatt cagcccgatc gtcgatttcg
     5641 atgaccgttc acttcatatt ccagaaaacc ttgaccgcat gctagttcaa tggctgggag
     5701 aaaaacctct aaaacaatat ctgcgagaaa cgttggatag acaaaaaatg gaagaggcag
     5761 gggtcagtga agaaactctt cttcgccaaa gtgaccacgt tacacaatgg ttaatagaga
     5821 ccttcaacca atcgtcaaaa aagaaacgcc gaattttatt attcgatact gtcgaaaaag
     5881 tggaggataa agtttggcaa catatttgtg acctgctcca acatgccgaa aatacattca
     5941 ttttgctggt cagcagaaaa aatgatcgcc tccttgacac attaaatagc aaaatcacga
     6001 aaagtattga gattatccag tgggagccat tggaagatga aaagtttggg gagttatatc
     6061 tcgatcagaa gcaacgttcc ttacatgtag ccctaccgcc aagcatatgt aaaaaacttg
     6121 tactcttggt tggcggaaga cccatcctta ttgatttgac cgtagaagca atttctcgta
     6181 atttaatgcc agagattctg acacataaaa cctatgcaga gataaaagcc ctttccagcc
     6241 ggaagagtgg gaaactgcga caagattttg aaactcaatt agtacgacag atcatggacg
     6301 tacgtgattc tatggatagg ctggttttgg tcatgtcgcg agtttatcca ttgtcgcaga
     6361 aaatggtcac agaattcctc aatatcacgg aaagagatgc caaaaatctt tttgaggagg
     6421 caaaactaaa gacaacgata aaaatattgc ccgatggaag aatatctctt cacgatgaga
     6481 tcagaaccat ggtcgaaaat tatgtttggc catacattga tccagatggt gaccggcaga
     6541 aacgtgatag ccgaattgcg atagattatt tgcggcacga agaagtcgaa atccaaaaag
     6601 atctaaaaaa gcttgaagat caggcttctg tagagaaaac tcaagacgcc ggtatttcgc
     6661 ttgatgtgat gtcaaagatg gaatctctaa aacaatattt atgggacgtc cgcgaacagc
     6721 ttcttgcaca ttcgttatat atcaacacgg aagaaggaat tcaaacattt gcagatatct
     6781 ttgaccgggc aacgcgcgca tatcacttcc catatcgtga ggtcttatta cggaaagttg
     6841 aaccatacat gccgcacctt ccggtagcgc ttaagtacga agttaatagc cgtaaagtta
     6901 aggcgtcact agatactggc gactacatcg aagcagacag acttacaaca caagtactga
     6961 aagacaaatc tattgctcct caacaacgtg tagatatgct tatccagcat ggaaatataa
     7021 agatccgatt gggaaagtta tcagagggga aagaaaagtt cgagaaagct gttcgtatct
     7081 gcaaagcagg caatgatatt cgtaaccttg tccctgcttt gaatgcgaga ggctgggggt
     7141 atcgccaagt tgggaatata gataaagcga ttattgacta tcaaaatgca ttggaactaa
     7201 gttttagtac tggcgatgaa ttgcgacgtg catggatact gaacaatttg gcatatgtat
     7261 acagtcagca gggacgattc aacccggcgc tagctctttg cgaacaggca aaggaacttt
     7321 ggaagaaaat cggttttggg cgtggtatgg gcgcattgta tgaagtatat gggcatatct
     7381 acatcaaaat gggacgctat gatgaagcca tggaatactt tcagctagcc ctagatgagt
     7441 tcaaatctcg tgatgtcgaa tggcaaagca gaatccacgc tgggctgggg tttgtatata
     7501 gaatgatgaa gaatcgcaaa aatgcagaac gtgaattgaa actcgcttta gactatcgca
     7561 ttcaaaaaga tcgggcaatg atcttgcacc gtatggcgca tcttcaattg gaaggcggaa
     7621 aaataaagaa agcaaaaaat ctttttgaac aaagttatga agccagccgc gaaatatctg
     7681 atgcatatca tgaactgaat aatctgggag accttgccaa actcgctgta ggtgagaagg
     7741 atttctcaaa cgcagcgaga tttgaaaagc tatatcagga atacaaaaca acctggccag
     7801 acatgaccta ctcgcgtgtg gaaggaatga tcctgagaca tttgggcgat cttatgttgg
     7861 gcaatccaaa aaacaaccta tctaaagcaa ttacttacta tcaaaaagcc ttcttgttgc
     7921 tagcacggta cgagacttat aaagagtact ctgtacaaaa acaacttgaa atacttgatg
     7981 cgtacttcca tgaattaaaa gtaccagcaa atgtaaaatc aaaaatcggg caatctctct
     8041 atttcgtttg gcagaaaaac aatctaatag aacatcatcc agaagcgctt ggtttctttg
     8101 cgcaatgggg aggcacagtt aaataacatg tttaataaat ttgcaaccca agaaacaccc
     8161 agtgttatct ttgaccatcg gcccgtggcg catgccacgg gccagtgcga cgcatgccct
     8221 cccacgttac cgatcttggc tgaaaagggc aatatgtttc ttgcaaatga atattatcct
     8281 gcttacataa cagcttcgct ggaattagat aaagggatgc atcttgtctg tacggctgac
     8341 tcttcacgcc cggctctttt aaatgaccaa ggctgggagc tattttcccg gatagtgaat
     8401 cagcaaccga taacaggctc gttggcaggc acagcctgtg gaagttttgg acaatcgttg
     8461 attgctgccg gacttttatt acccggaagt agaaatgcaa cacccaaaga acaaaatcat
     8521 attttgacat cttggctgca cctaacagat cgttgtaatt tacgttgttc ttattgctat
     8581 cttccacatg tgcttgagga tatgtcactt aaaacgggtt atgtcgccat ggatgccatc
     8641 ttccgctcgg cgttggcaca tggattcaaa caagtcaaga tcaaatacgc aggtggtgaa
     8701 ccgctcttgt gtttcgcgac attaataacc ttacatacct atgctcaaca acttgcagaa
     8761 aagcatagac tcgatttgga cggtgttatc ttaagtaatg gaaccctcct gacagtggag
     8821 atggttgaga tgctgaaaac atttgatttg cgtctcatga tttctctcga tggattgggt
     8881 aattatcacg atagtcaccg accctatgca ggcggacgag ggacattcgc agatgtatca
     8941 gaagccattg acctggcatt ggcgcatgga gtcgtaccag acatttcagt aacagtcagc
     9001 aaccgcacag cagatggatt ggcgaatgtt attgcatgga ttctggaccg taatctgcca
     9061 ttcagcttga acttttatcg tgagaatgaa ttgtccgttt caaataccga tatgcgactg
     9121 gacgatgaaa gaattgtccg tggagtactt gccgcttaca gggtcattga agatcgaata
     9181 cctcaaagaa gcttgctcgc cgcgttgatc gacagggcaa acctcgctgt cccacata
//
LOCUS       DAOREF010000004         9067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_3623_length_9067_cov_4.332889, whole genome shotgun sequence.
ACCESSION   DAOREF010000004 DAOREF010000000
VERSION     DAOREF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3623_length_9067_cov_4.332889"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PKI33_00155"
     CDS             <1..719
                     /locus_tag="PKI33_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35434.1"
                     /translation="YGKYNPVKLFQWFEQRRYKKAVAELDKLDDEYRECYAAKKEFPA
                     KSRTRRNEIMRQLAEEFPDKEEYLLPTPFGNVLRSFEIYPRVMYGLEAIDGWGRLLAV
                     VPKDYTELIDSAKSQVDFWVNMAVVLVLLQIEYLGLAYIFNHGDVNWWIVLLLIAIGV
                     IPPIRATSAAKEWGDLIKSAFDMFAPQMREALGFDVPADRDEEKAQWTKFSQAIIYRL
                     PERIPGLKKEEKKSPPKRKK"
     gene            729..2846
                     /locus_tag="PKI33_00160"
     CDS             729..2846
                     /locus_tag="PKI33_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM35435.1"
                     /translation="MANQFTWTPLYQELAKELVNWQSRQGDLITFLEGLRANDYVITP
                     FMDRDENGERFPLTEIDPFTFFGVFNRGIKKEQRIAILAEMKKFFGLSSSLPVDFDGI
                     PVLNNMSSWFISFRGKRKEDDTAKLWRVFLLALGESPLKSQQFLSAFDDALTVRRTNI
                     NLTIGLFWIRPDTFLNLDSTNRAYLGIPFKAGIFNANYYAEVTEKVRAQGMPFTQISF
                     EAWEVGGQAEDVRAGGTPSLNVEDESVNYWLVGAYWDNRDPANQTARFLEEGIWENGY
                     DNKYGDEVNSMKVGDKIAIKASATQRKGLPFDNKNITTSRMIIKAVGTIVANRKDGRT
                     VEVEWDPNFQEKDWYFYTSRNTIWHVRPPGKNIWTEAANQLIDFVWYGKPQNYDWFTH
                     QWWGSHTEVPNDDTNIQVEPYGVEDILASGVFLSEEEIKHILDRAYEKKALIIQGPPG
                     VGKTFIAQKLAYALMKEKAPACLEMVQFHQSYSYDDFVRGYRPLAGQPGTFGLQNGVF
                     YEFCQKAINDPEREYVFIIDEINRGNLSQIFGEMLMLIERDKRGSEYSVPLVYRDQPN
                     ERFYIPSNVYLIGLMNVADRSLAMVDYALRRRFAFVNLRPQYESDMFHKWLSNNMMNA
                     TLIQLIVERMSALNKVIREDPLLGENYQVGHSFFCPKGNSFSELDSTWYRGIVRTEIV
                     PLLKEYWFDNPKKSEDAERNLLA"
     gene            2843..3958
                     /locus_tag="PKI33_00165"
     CDS             2843..3958
                     /locus_tag="PKI33_00165"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35436.1"
                     /translation="MMAAGSAATTNEEIFWTEYGIPIRNLWYMLLYAWNETPATVSSS
                     LKDVEDAPTLDALLATVLMKLIQQRLRIGLGRNYLDEKRLLRGIRGRINFTESLKSRA
                     FDRGQAYCAVQQYSVNVPKNQIVQSTLMRLVQVGNFGPDRQLAEELRHNLRWTTRLLD
                     GVDLIELKLDFIRRQQVGRNDRDYSLMLAICELLLQRQMPMDNTDGIYRLPDLDRAAL
                     TLHHVYERFVANFYRLHLPDWNVSPQKAIVWHDSTNNKYLPAMRPDIFMEEKSTGRII
                     ILDTKFTAHSLIANPWSSEKFDSAHLYQVYAYLKTQEHLSDQHRHAAGVLLYPAVKQS
                     FLSEKTEIQGHAFRVETVNLTMAWQAIEQRLLDIVRI"
     gene            complement(4544..5041)
                     /locus_tag="PKI33_00170"
     CDS             complement(4544..5041)
                     /locus_tag="PKI33_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35437.1"
                     /translation="MRAGFFTLLVILVGFGFLSSEVYHKSQELSSANDKIWSLEGELQ
                     TAKDQNEASLADIENLRTQISKLEEEKKQLETQIENFQLENSLLRGQNIQIQWENNQM
                     ATYSPALSYLIKMISTNESRTMATSVLPLAIGVLVIARRKYSRCKITQTHKRMHKERQ
                     GKLLI"
     gene            complement(5086..5373)
                     /locus_tag="PKI33_00175"
     CDS             complement(5086..5373)
                     /locus_tag="PKI33_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35438.1"
                     /translation="MKHIKHKTHTKAKPRKNEGILNMIDRMIKSIIGGIIGLIVAFLF
                     GTVVVVLAAKAYTLSDDLSYQLLHSVINLIGTLLGAVLGYVFHKNNASGKE"
     gene            complement(5437..5919)
                     /locus_tag="PKI33_00180"
     CDS             complement(5437..5919)
                     /locus_tag="PKI33_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35439.1"
                     /translation="MKTKKNIKEGAVDAARDDSQVETLSSPRNYTVQNNWLKSIIGED
                     QVTTQRQMRCLLAEQFLLTILYPLLQQKVNITQDILVAALIEFKKEYTTVGIDFEISN
                     VTGRLLERFDCYVAEEFDPTADNAETFNRDIELFIILGDLDLTEVDERVKKALLKNVI
                     "
     gene            complement(6219..6887)
                     /locus_tag="PKI33_00185"
     CDS             complement(6219..6887)
                     /locus_tag="PKI33_00185"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNM35440.1"
                     /translation="MISSSYQPNMFHLAKVAYCQPGNLLQHSQNVAVLAADLAQSYGC
                     NKLEKDQIFMGCLFHDIGKQFIPSEVINKKGRFTTEEFEDIKLHTWKGYNYLINLISD
                     PVILQTVLYHHERWDGSGYPYGLASNEIPLCARICAVVDVWDALISDRCYRSAWDLTQ
                     AFELIWTGGGSLFDPDIAFLFLEMIEKKSSNGQLVKNVSLKIKPRHPEFLRNPGTYKG
                     INPN"
     gene            complement(6889..7485)
                     /locus_tag="PKI33_00190"
     CDS             complement(6889..7485)
                     /locus_tag="PKI33_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM35441.1"
                     /translation="MFQFLRIDFQSEVPMADIVLTPEEITIKINAANKIASQLLPSHL
                     GGATNEDLVGEYLLKEIEQGRSLSEILKTLNTPKLLRWCLRNTKTDRFRKESAMKRGG
                     KNPKIPFEEVESIHKSSFEQPEAALIKKEESSFLEETIQKSGLSETQIKILGFFRKGY
                     KTNEIASELNMSVEAVSARKSEAVHKLSRILKQLKQGA"
     gene            complement(7834..8481)
                     /locus_tag="PKI33_00195"
     CDS             complement(7834..8481)
                     /locus_tag="PKI33_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM35442.1"
                     /translation="MRPPANNKIIKVAIFEDSRFDLRAIESVLKEIPGIQILGNFDTI
                     NEAIQSCKNLEPDLILVDADIRGDRQAGPLFAQSIKRILPRVKMLGLTKYSESIAQLR
                     LAGCQEAVLKSFFDNSAVAAKFIKEALLEPPAYFKDDLPPILNPEEDMVLKMIATGKT
                     ENEIADRMSKTKRQIKDIKESLKLRFGAASNKDAQLISNAYQIRYLKPEDDLSQY"
     gene            complement(8486..>9067)
                     /locus_tag="PKI33_00200"
     CDS             complement(8486..>9067)
                     /locus_tag="PKI33_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35443.1"
                     /translation="VNDLVRDAPLNSETYEILKKSTSFVSDQMDNILIEPVNYFYLEN
                     DGLLSALEKYFRLLQDGMPNFRSRILYDYGGVAEVIPYDIKTALFNIGREAIMNALRH
                     SKINLLDNGEIRVTFIKEDTEKTAYVLKISDNGVGFSVDEKRKTITSYGLVDMFENQQ
                     MILKKSGDKSKVTIDSRITYGTTVTATWAPGRN"
BASE COUNT         2485 a         1964 c         1928 g         2690 t
ORIGIN      
        1 gttacggcaa atacaatccc gtcaaactct tccaatggtt cgagcaacgc cgctataaaa
       61 aagcggtcgc cgaacttgat aaactggatg acgaatacag ggaatgttat gccgccaaaa
      121 aggaattccc cgccaaatca cgcacgcgca ggaacgagat catgcgtcaa ctggcggagg
      181 aatttcctga taaggaagaa tacctgctgc ccacgccatt tggcaatgtg ctgcgctcgt
      241 ttgaaatcta tccgcgcgtg atgtatggct tggaagccat tgacggctgg ggcaggctgc
      301 tcgctgtagt cccaaaggat tacaccgaac tgatcgactc cgccaaatcc caggtggatt
      361 tttgggtcaa tatggcggtt gtgttggttt tacttcaaat cgaatatctt gggcttgcct
      421 atattttcaa tcatggcgac gtcaactggt ggatcgtgct attgctcatt gccatcggcg
      481 tcattccgcc catccgcgcc accagcgccg ccaaagaatg gggcgacttg atcaagtccg
      541 cttttgatat gttcgctccg caaatgcggg aagcgctcgg gttcgacgta cccgccgatc
      601 gggatgagga aaaggcgcag tggacaaagt tcagccaggc gatcatttat cgcctgcccg
      661 agagaattcc tgggttgaag aaagaagaaa agaagagtcc gccgaagagg aagaagtaag
      721 aggcaaatat ggcaaatcaa tttacctgga ccccgttgta tcaagaactt gccaaagagt
      781 tggtgaactg gcaaagccgc cagggagacc taatcacttt tctggaaggc ttgcgtgcca
      841 atgattacgt gatcacgccc tttatggata gggatgagaa tggcgagcgt ttcccgttga
      901 cagaaattga tcccttcacc ttctttggag tattcaaccg cggaatcaaa aaagaacaac
      961 ggattgccat ccttgctgag atgaagaagt tctttggcct tagcagttcg ttgcctgttg
     1021 attttgacgg cataccagtg ctcaacaaca tgagttcatg gtttatttct tttcgcggga
     1081 agcggaaaga agacgatacc gccaagctgt ggcgcgtctt tttattggca ttgggcgaga
     1141 gtccgctcaa gagccagcag tttctcagcg cgttcgatga cgcactgact gtacgcagga
     1201 ccaatatcaa ccttacgatt ggactctttt ggatacgccc cgataccttc ctgaatttgg
     1261 acagtaccaa tcgcgcttat cttgggatcc cgttcaaagc gggtattttc aatgcgaact
     1321 attacgcgga agtcactgag aaagttcgcg cacaagggat gcctttcacg caaatttcct
     1381 ttgaggcatg ggaggttggc gggcaggcgg aagatgtgag ggccggggga actccctcct
     1441 tgaacgtcga agacgagagc gttaactatt ggctggttgg tgcttattgg gataatcgtg
     1501 acccagccaa ccaaacggca cgttttcttg aagaaggtat ttgggaaaac ggatacgaca
     1561 ataagtatgg cgatgaagta aactccatga aagtgggcga taagatcgct atcaaagcgt
     1621 ctgctacgca gcgcaaaggc ctgcctttcg ataataaaaa cattaccact tcccgtatga
     1681 ttatcaaggc ggttggtacg attgttgcca atcgcaaaga cggaagaact gttgaagtcg
     1741 aatgggatcc aaattttcag gagaaagatt ggtatttcta cacaagcaga aacacaattt
     1801 ggcatgtccg accacctgga aaaaatattt ggacagaagc tgcaaatcaa cttatagatt
     1861 ttgtttggta tggaaaacca caaaattatg attggtttac ccatcaatgg tgggggagtc
     1921 ataccgaagt tccaaatgat gatacgaata tccaggtaga accgtatggg gttgaagata
     1981 ttcttgcctc tggcgtcttt ttgagcgaag aagaaatcaa acatattttg gatcgcgctt
     2041 acgaaaagaa agcactgatt attcaaggtc cgccgggtgt tggcaagacg ttcattgccc
     2101 agaaattagc ctacgcactc atgaaagaaa aagcccctgc ttgtctcgaa atggtgcagt
     2161 ttcatcaatc ctactcgtat gatgattttg tgcgcggata ccgtccactg gctggtcagc
     2221 ccgggacctt tggtttgcaa aacggcgttt tctatgaatt ttgccaaaag gcaatcaacg
     2281 atccggaacg tgaatatgtc ttcatcattg acgaaatcaa ccgtggaaat ctcagccaaa
     2341 tctttggaga aatgcttatg ctgatcgagc gcgataaacg cgggtcggag tattccgttc
     2401 cacttgtata tcgagaccag cccaacgagc gtttttacat cccctctaac gtatacctga
     2461 tcggactgat gaatgtggca gatcgttctc tggcaatggt ggattatgcc ttgcgtcgcc
     2521 gctttgcttt cgtcaacctg cgcccacagt acgagagtga catgttccat aaatggctat
     2581 ccaataatat gatgaatgct acactgattc agttgatcgt tgagcggatg tccgcgctca
     2641 ataaggttat tcgggaagac cccttgcttg gcgaaaatta tcaggttggg catagtttct
     2701 tctgccccaa aggcaatagt ttctcggagt tagactcgac ttggtatcgc ggtattgtgc
     2761 gtaccgagat cgttcccctg ctaaaagaat attggtttga caacccgaag aaatctgagg
     2821 acgcggaaag gaacctgctc gcatgatggc tgccggcagt gccgcaacca ccaacgaaga
     2881 aattttttgg acagagtacg gaattcccat ccgtaacctt tggtatatgc ttttgtatgc
     2941 atggaatgaa acgcccgcca ccgtgtcgag cagcctgaaa gatgttgaag acgcccccac
     3001 gttggacgct ttacttgcca ctgtgttgat gaagttaatt caacagcggc tgcgcattgg
     3061 gctggggcgc aattacttgg atgagaaacg cctgctgcgg ggaattcggg gacgcattaa
     3121 ttttaccgag agtctgaaga gcagggcttt tgaccggggt caggcgtatt gcgccgtcca
     3181 gcaatatagc gtcaatgtcc ccaaaaatca gatcgtccaa agcacgctga tgaggttggt
     3241 gcaggtcggg aattttggac cggaccgcca actcgcagag gaattgcgtc ataacctgcg
     3301 gtggacgacc agactgcttg acggtgttga tctgattgaa ctaaaactgg attttatccg
     3361 caggcagcaa gtcggacgta atgacagaga ctacagcctt atgctggcaa tctgcgagtt
     3421 actgttgcaa cgccaaatgc cgatggataa tacagatggt atttacagac tcccagacct
     3481 ggatcgagca gcattgactc ttcaccatgt ttacgagcgc tttgtcgcaa atttttaccg
     3541 cctgcacctc cctgattgga atgtttctcc gcaaaaggca atcgtatggc acgacagcac
     3601 caacaacaaa tacctgcctg ccatgcgccc cgatatattt atggaagaaa aatccacagg
     3661 gcgtattatt attctcgaca caaagtttac agcccatagc ctgattgcca acccatggag
     3721 cagcgagaaa tttgactctg cgcacctgta tcaggtgtat gcctacttaa aaacgcagga
     3781 gcatttgtcg gatcagcacc gccatgctgc aggcgtcctt ttgtatcccg ccgtgaagca
     3841 atcttttttg tctgaaaaaa cggagataca aggtcacgct tttagagtcg aaacagtgaa
     3901 cctgactatg gcttggcaag ccatagagca acgcttgcta gatatagttc gaatttgaaa
     3961 cattaaaacg ttttagacaa gacagcgtga atctaagaag cgcgcaaatc tatttggttc
     4021 aaagaattaa gccgcgcccg ttgggtaacc tgccggacga atgcgccctc gatccattcc
     4081 gtatggaatg ggcatttccg gttggaattg gttacttttc cggcttaatt ttgggatcac
     4141 aatatgtctg ctaacagaca aataacagac aaatctgcgt tagagcggcc aacccattgc
     4201 cttcggtgga tatgatcggg gcaagtagat tttgaatttc gggatttatt cggctattgt
     4261 tgtgtttatt atcctacgcc tcgatcattc atacagaata gccgaattct tttagaatac
     4321 aaaacatgtc tatactttag gctctccccc aaatattatg ggatccaaaa aagtccgttt
     4381 ttgcctggag ggatgaactt atcatggagc gaatacgact cgcctctgaa ataaggtcgc
     4441 acaatagatg ttgtgagacg taaagatact aaatctatta gttttaacga tttgttcttg
     4501 ctaggataag gaactggtcg gatattccga ccagttcctt gattcagata agcagtttcc
     4561 cttgcctttc cttatgcatt cttttatgtg tttgtgtgat tttgcacctg ctatattttc
     4621 gcctagcaat tactaatacc ccaattgcaa gtggtaatac cgaagttgcc attgtacgag
     4681 attcgttagt ggaaatcatt ttaatcagat aagataacgc aggtgaataa gttgccatct
     4741 gattattctc ccattgaatc tgaatattct gtccgcgcaa taacgagttc tccaactgaa
     4801 agttctcgat ttgtgtctcc aattgtttct tctcttcctc caatttagaa atctgcgttc
     4861 taaggttctc gatatctgca agagatgcct cattctgatc tttagcagtt tgaagttctc
     4921 cttcaagcga ccatatcttg tcgttggcag aggaaagctc ttgtgattta tggtagacct
     4981 ctgatgaaag aaagccaaag ccaacaagaa ttacaagcag ggtgaaaaat ccggcacgca
     5041 tggtattttc tcctttcttt ataaatgtcc aaaagaattc cgtgatcatt ctttccctga
     5101 ggcgttgttc ttatgaaata cgtatccgag cactgcgccc agtagtgtgc caatcagatt
     5161 gattaccgaa tgcaacaatt gatatgagag atcatctgac agtgtgtatg cctttgcagc
     5221 taacactact acaacagtac cgaacagaaa cgccactatg agaccgataa tgcccccaat
     5281 gatcgacttg atcattcggt caatcatatt aagaattcct tcattttttc tcggcttggc
     5341 tttggtgtgt gttttgtgct tgatgtgttt cataggacat ctccttctga tctccattaa
     5401 tacctacgaa acacttgaac aaatcctaca attttattag atcacgtttt tcaggagagc
     5461 cttcttaact ctctcatcca cctccgtgag atccaaatcc ccaagtatta taaatagttc
     5521 tatgtcccta ttgaaggttt ccgcattgtc agctgtgggg tcgaattcct cggctacata
     5581 gcaatcaaaa cgttccaata atcttcctgt aacatttgaa atttcaaaat caataccgac
     5641 tgttgtatat tcctttttga attctattaa cgcagccacc aaaatatcct gggttatatt
     5701 gaccttctgt tgcaataatg gatataagat tgtcaacagg aactgctctg ccaataggca
     5761 tctcatttgg cgttgagtcg tcacttgatc ttccccaata attgatttga gccaattatt
     5821 ttgaactgta taatttcgag gagacgacag agtctctacc tgagagtcat cgcgagctgc
     5881 atctacagca ccctctttta tgttcttttt tgttttcatt tatatctcct ttatttttca
     5941 tgcacttaca acaacgtaaa aactgatgaa tacattaccg gaaaagtcca aatatcctta
     6001 cattcttgtc aggctagcat agcaattgtt gatattgaag tttggaatgg atctgtttcc
     6061 agttgtcaaa tatatatgaa taagtttggt catgtgagca tctaatgcca agatactaag
     6121 atataaagtt tgcctaatga aagcagaaca tataggctgt tcgacccatt caaaataatc
     6181 tcgttgggtg atttttctta atcgacaact ttatatcctt aattagggtt aattccttta
     6241 tatgtaccgg ggtttcgcaa gaattccgga tggcgtggtt ttatttttag acttacgttc
     6301 tttactagtt gaccgtttga tgatttcttt tctatcatct ctagaaataa gaacgcaata
     6361 tccggatcaa aaagactccc tcccccagtc catattaatt caaatgcttg tgtcaaatcc
     6421 catgcagacc tgtaacatcg gtctgaaatc aaagcatccc aaacatctac taccgcacat
     6481 atccgtgcgc aaagaggaat ttcattagat gccagtccgt atgggtatcc acttccatcc
     6541 caacgttcat ggtgatacaa aactgtctgc aaaataacgg gatctgatat taaattaatg
     6601 aggtagttat aacctttcca ggtgtgaagc ttgatatctt caaactcctc ggttgtaaac
     6661 cttcccttct tgttaattac ctcggatgga ataaactgtt tgccgatatc atgaaaaaga
     6721 catcccatga agatttggtc tttttctagc ttattgcaac catatgattg tgccaagtct
     6781 gcagcaagaa ctgctacatt ttgagagtgt tgtagtaaat taccaggctg acaatatgct
     6841 actttcgcta gatggaacat atttggttga taggaactag aaatcatctt aggctccttg
     6901 tttgagttgt ttcaagattc ttgacaattt atgtactgct tccgatttgc gggccgaaac
     6961 tgcctcaaca ctcatattaa gttcagaggc gatttcgttg gtcttatacc ctttccgaaa
     7021 gaagcctaaa atcttaatct gagtctcact taatccagat ttttgaattg tctcctcaag
     7081 gaaacttgat tcctcttttt ttatgagcgc agcttctggt tgctcaaatg atgatttatg
     7141 gatggattca acttcctcaa aaggtatctt cggatttttg ccacctcttt tcatggcgct
     7201 ctcctttcga aaacgatcag ttttagtgtt ccgtaaacac cagcgcaaca atttaggggt
     7261 attgagcgtt ttaagaattt cagaaagact tctgccttgt tcaatttcct ttaagagata
     7321 ctccccaacc aaatcttcat tagttgcccc tcccagatgt gagggtagca gctggctggc
     7381 aattttattt gctgcattaa ttttgatggt tatttcttca ggagtgagaa ctatatcggc
     7441 cattggaacc tcgctttgga aatcaatacg caaaaattga aacaaactta caaaaaagag
     7501 caggcgactt tatagtcgcc tgctctttgc agaaaaaggt taagaaccca ccaatggcaa
     7561 gttatgcctt ttaatctttg ggatcgtttt ttgtgagata gtttgaattc atttattgcc
     7621 catacactct ttttaccaat gtcgaagaag ggcatgcaac atatctggtc gtaagttagt
     7681 attggaacga gagaaaacat tttcactatc tccatttgac gaatcgaatt attggagata
     7741 gattaattta ttgaacctta aattggggtc cgtaaaaaat aaaacaaaaa gccctctgag
     7801 ggctttttgt ttggactttt gtccatctac tgcttaatac tgggataaat catcttcagg
     7861 tttcaggtac cttatttgat aagcattaga aatcagctgt gcatctttgt tactagcagc
     7921 accaaatctc aattttaagg attctttgat gtcttttatc tgcctttttg tcttactcat
     7981 gcgatctgca atttcatttt ctgtttttcc agtagcaatc atcttaagta ccatatcttc
     8041 ttccggattt aatataggag gtaggtcatc tttgaaatat gcaggtggct ccagtagggc
     8101 ctctttgatg aatttcgcag ccacagcact attatcaaag aaagacttta aaacggcctc
     8161 ttggcagcca gctagtctta attgagcgat ggattctgag tattttgtta atcctaacat
     8221 tttgactcta gggagaattc tttttatcga ctgcgcaaat agtggacccg cttgtctatc
     8281 acctcttatg tcagcgtcca caagaatgag atctggttct aaatttttac aactttggat
     8341 agcttcattg attgtgtcaa agttacctaa gatctggatt cccggtatct ctttcaaaac
     8401 gctttcgatg gctctaagat caaatctgct atcttcaaaa atagcaactt ttataatctt
     8461 attatttgca ggaggcctca ttttttcaat tccttcctgg tgcccaagtt gccgttacag
     8521 ttgtaccgta ggtaattcta gagtcgattg ttacctttga tttatcacca gatttcttaa
     8581 gtatcatctg ttgattctca aacatatcaa ccaagccgta acttgtgata gttttgcgct
     8641 tctcgtcgac cgaaaaccct accccattat cagatatttt tagaacatag gcagtttttt
     8701 cagtatcttc ctttatgaaa gtcactcgga tttctccatt atcaagcaga ttaatttttg
     8761 aatgccttaa agcattcatt atcgcctctc gtccaatatt aaatagtgct gtttttatat
     8821 cgtaaggtat gacttctgct acgccaccgt aatcatataa aattctactg cgaaaatttg
     8881 gcattccatc ttgcagtaaa cgaaaatact tttccaatgc agacaacaac ccgtcattct
     8941 ctagataaaa atagttgaca ggttctatta aaatgttatc catctgatct gaaacaaatg
     9001 aagtgctctt tttcaagatt tcataggttt cagaattcaa gggcgcatct cgcactaggt
     9061 cattgac
//
LOCUS       DAOREF010000005         8171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4296_length_8171_cov_3.828857, whole genome shotgun sequence.
ACCESSION   DAOREF010000005 DAOREF010000000
VERSION     DAOREF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_8171_cov_3.828857"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PKI33_00205"
     CDS             <1..1248
                     /locus_tag="PKI33_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNM35444.1"
                     /translation="VEDAQGSKAPIQKMADQVSAVFVPAVIAIATLTFLGWYFFGPTL
                     PINADVDNFTRALIYMVAVLVIACPCAMGLATPTAIMVGTGKGAEAGILFRNSEALER
                     AGKVSVVVLDKTGTITKGQPAVTDIVTSNVTSEELLRLAASVEKGSEHPLGEAIWAEA
                     TTRGLTLVEPSGFKAEAGHGVQAEVEGRSVMVGNKRMFEARGISLGSLESEVTRLQSE
                     AKTAMLVAVDNVVAGIIAVADTIKDSSKDAIADLHKMGLKVAMITGDNQKTAEAIAKQ
                     VGVDTVLAEVLPEGKSAEVKKLQSNTSVVAMVGDGVNDAPALAQADVGLAIGTGTDVA
                     MAAAPVTLMSGDLRGVSRAISLSRKTVTTIKQNLFWAFIYNVILIPAAMLGYLNPMLA
                     AGAMAFSSVFVVTNSLRLRSVKI"
     gene            1298..1516
                     /locus_tag="PKI33_00210"
     CDS             1298..1516
                     /locus_tag="PKI33_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35445.1"
                     /translation="MTDLSQVPQQQEIKSACGGILNDPSAYPSAMFNGERVYFCNAAC
                     LKAFLLAPEAFMAGEVEHPLEDVDESKI"
     gene            1534..2043
                     /locus_tag="PKI33_00215"
     CDS             1534..2043
                     /locus_tag="PKI33_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35446.1"
                     /translation="MKKIAVLLFTALLVTLTACAPKAAEMPKAELPAGKAWADGKEIY
                     FIHTEASDLGVAEKLTNMMKSPVILVPSLANVPDESLANVYVFTNGIAGSGPFGFQAD
                     VFDNPPSTDGYTPLRRLNAVTWTDETKARELTSAADVLAAESAGELIIEQPGVVINMP
                     FVVWDGGQR"
     gene            2116..3300
                     /locus_tag="PKI33_00220"
     CDS             2116..3300
                     /locus_tag="PKI33_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNM35447.1"
                     /translation="MSKNQKPSVDPVIIVMLGVIFLAVAIAMIVFQPTSNSINLTADA
                     SVMSQLVVAFVTGITTGGLSCLAVQGGLLASSLAYQIEQDYSAQAKNKKATRTNSAMP
                     IALFLIAKLVAYTLMGALLGWLGSFLTLSPLTRAILMIAIGIFMVGNALRMFNVHPIF
                     RYFSIEPPKFITRYIRRTAKGTDTATPIFLGLLTIFIPCGVTQAMMATALGTGSVLMG
                     AALMFAFTLGTSPVFFIVAYLATELGAKLEKFFMRFVAVVVLILGLVTLDGGLNLMGS
                     PLSLQNMTRGWFAPQAESNAITESVQPSAASDEIILSVENNGYFPAILKAPAGKDLTL
                     SLVTNKTYSCARDFVIPDLNFYELLPDTGTVQVNIPAQKAGSTLFFTCSMGMYTGQIV
                     FE"
     gene            3334..3546
                     /locus_tag="PKI33_00225"
     CDS             3334..3546
                     /locus_tag="PKI33_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNM35448.1"
                     /translation="MIKKTFKIPDMSCTNCAMKIESLEDDLDGVKEVNASYHKLEMVI
                     EYDEAKLTDEQIVTAVKKKGYTAVAA"
     gene            3681..4064
                     /locus_tag="PKI33_00230"
     CDS             3681..4064
                     /locus_tag="PKI33_00230"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNM35449.1"
                     /translation="MPKYYTPKEANNLLEIVRPMVGDLMQIGERVRARGPEIWAMVEK
                     SAGNGGNPELSKMLPDFDRLDEILHRLQDMEIEVKDLASGLIDFPALRDGTVVYLCWK
                     YGEGSIQFWHEIEAGFAGRQPIDWE"
     assembly_gap    4265..4364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4365..5089
                     /locus_tag="PKI33_00235"
     CDS             <4365..5089
                     /locus_tag="PKI33_00235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM35450.1"
                     /translation="VKVLMYSRTLDTVYRDFFQNEAEVIAALEHPCIVPIYDFGWHGQ
                     QPYIVMRYMAGGSLQDHLEKGEIKLTEMAHIFKRVADALDAAHAKSIIHRDIKPSNIL
                     FDASGEAFLSDFGIATSKSIKDDDGEWLVGTPAYMSPEQAKGGHVDGRSDVYALGVTL
                     YRLLTGQLPFSSDSATSLINSHAELPIPDIRSVKSNIPAVWQEVVSKAMAKDPKDRYA
                     TAGDFARDVNEVVSGKWFLRKL"
     gene            5183..6079
                     /gene="rarD"
                     /locus_tag="PKI33_00240"
     CDS             5183..6079
                     /gene="rarD"
                     /locus_tag="PKI33_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNM35451.1"
                     /translation="MKKGIWYGIAAYAMWGFFPIYWKLLHDIPALQLLGHRISWSFLL
                     LFAFIFITKQWDEFRAVAFNRRTLGIYAVAGVLLSLNWLIYVWGVNAGFIVETSLGYF
                     INPLLSVLFGVIFLREKLRPMQWLPVVIATIGVTYLTITYGRLPWIALSLAVSFGLYG
                     LVKKLSPLGSVFGLTLETGIVFPVALIYLAFVQVNGTAGFLHDGALVDILLIGGGIVT
                     TIPLLMFASAAKEIPLNMVGVLQYFAPTIQFLIGVFVYKEPFDHTRLIGFGIVWLALI
                     IFGLENILAHRTPPQTIPELGE"
     gene            6082..7080
                     /locus_tag="PKI33_00245"
     CDS             6082..7080
                     /locus_tag="PKI33_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM35452.1"
                     /translation="MKLLFIGGTGLISSACSELAIERGHELFLLNRSASKIYPPPKGA
                     TVLQADIYADPAHLSTLLAGQRFDAIVDFIAFHPYDIQRDLSLFRDKTDQFVFISSAS
                     AYQKPVRKYPITEETPLENPFWEYSRNKIAAENLLMDEYHKNGFPVTIVRPSHTYGPS
                     QIPFSYGSWRHQWTLIHRMKQGQPVIMPGDGTSLWVLTWNADFAKGLLGLLGNKKAIG
                     EAFHITSDEALSWDQIYFEMYQALGADPNVIHIPSDYIARFDPEATGTLIGDKSNSVI
                     FDNSKIKRFVPDYHCEVTWREGFRRSLAWFEAHPEFQTIDEDALQNWDRIIQAYTR"
     gene            7214..>8171
                     /locus_tag="PKI33_00250"
     CDS             7214..>8171
                     /locus_tag="PKI33_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNM35453.1"
                     /translation="MNFAMWKKALNVIPEVSKEEWDGLDIISKWLISTRAAVLIMTFI
                     SAALAGLFAWRDGSFSFLPWLALVFGLIMAHASNNIFNDYTDFVRGVDKDNYYRTMYG
                     AQPVASGLMTKRQHLTYFVVTGILAMVAGLYLVWQTEFNTTTWILLGLGSFFVLFYTW
                     PLKGLALGEVAVLMVWGPLMIGGGYYVLTGNWNWSVVIASLPYVLGVTTVIFGKHIDK
                     IKVDQAKKIYTLPVVIGEKAARYTIIAMMVLPYLFTGYLIATKFFTPVMLIVLFALPA
                     LRESLPPLTKPKPETRPEGFPEGQGGWPLYFAPIGFRNNRAFG"
BASE COUNT         1920 a         1969 c         1978 g         2204 t
ORIGIN      
        1 gtggaagatg cgcaaggcag caaggctcct atccagaaaa tggcggatca agtctccgcc
       61 gtgtttgtcc ctgcagtcat tgccatcgct acgctgacgt ttttaggctg gtacttcttc
      121 ggtcccaccc tacccatcaa cgcggacgtg gataacttta cccgtgctct aatttacatg
      181 gttgctgttt tagtgattgc ttgtccatgt gcgatggggc ttgccactcc gaccgccatc
      241 atggtgggaa cgggcaaagg cgccgaagcg ggtattctct ttagaaacag tgaagctttg
      301 gaacgcgctg gaaaagtctc tgtggttgtg ctcgataaaa ccgggacgat tacgaaaggt
      361 caaccagcgg ttacagatat cgtaacgagt aacgtaacga gtgaagaatt gttacgcctt
      421 gctgcatctg ttgaaaaagg tagtgaacat ccgcttggcg aagcgatctg ggcagaagcc
      481 accacccgcg gactgacatt ggttgaaccg tctgggttca aggctgaggc tgggcatggc
      541 gttcaagccg aggtcgaagg aagaagcgta atggtcggga acaagagaat gttcgaggcg
      601 agaggaatct ccttgggcag tctcgaatcc gaagtgacgc gtcttcaatc cgaagcaaag
      661 acagccatgt tggtcgcagt ggataatgtc gtagcgggaa tcatcgccgt ggcagatacc
      721 atcaaagaca gctcgaagga tgcgatcgcg gacctgcaca agatgggtct gaaagtagca
      781 atgatcaccg gcgataatca aaaaaccgcc gaagctatcg ccaagcaggt tggtgttgac
      841 accgtgcttg ctgaagtttt acccgaaggc aaatcggctg aagtgaagaa acttcaatcg
      901 aatacgtcgg ttgttgcgat ggtgggggat ggtgtgaacg acgcccccgc tcttgcccaa
      961 gccgatgttg gtttggcaat tggtacaggc accgacgtcg ccatggctgc tgcgcctgtg
     1021 accttgatga gcggcgacct gcgcggagtt tcgcgtgcga tcagtctttc ccgcaagacg
     1081 gtgacgacca tcaaacaaaa tctgttctgg gcattcattt acaacgtgat cctgatccct
     1141 gccgccatgc ttggatactt gaacccgatg ctcgccgctg gtgcgatggc gttcagcagt
     1201 gtcttcgtgg tgacaaatag tctgcgattg cgaagtgtga agatataaat catcaggtga
     1261 ctgtcacttt taaagtgact ttcacctgaa tcttattatg accgatctat ctcaagtacc
     1321 tcaacagcaa gaaataaaat ccgcctgtgg cggaatcttg aatgacccat cggcttaccc
     1381 tagcgcgatg tttaacggag agagagtcta cttttgtaat gccgcctgcc tgaaagcatt
     1441 tctgcttgcg ccggaagctt ttatggctgg agaggttgaa catccgcttg aggatgtcga
     1501 tgaatccaaa atttgacaaa aggaactctt aaaatgaaaa agatagctgt actactgttt
     1561 actgcattgc ttgtcacttt gactgcatgt gctcccaaag ctgctgaaat gcccaaagcc
     1621 gaattgcccg ctggcaaagc ctgggcagac ggcaaggaaa tctatttcat ccatactgaa
     1681 gcttcagacc tcggtgtggc tgaaaagctc accaacatga tgaagtcacc cgtcattctt
     1741 gtgccttcgc tggcaaatgt gcccgatgaa tctttggcga atgtttatgt attcaccaat
     1801 ggcattgcgg gttcaggtcc attcgggttc caagccgatg tctttgacaa ccctcccagc
     1861 actgacggat atactcctct tcgccgcctc aatgctgtga catggacaga tgaaaccaaa
     1921 gcccgcgaac taacctccgc tgccgatgtt ctcgctgcag agtctgccgg tgaattgatc
     1981 atcgaacaac ccggtgtagt catcaatatg cccttcgtcg tttgggatgg cggtcagcga
     2041 taataaactt atgtacggct tacgcattac aaatttgcgt acgccgtaca tgtgtaaaaa
     2101 ctttattgag gtcgaatgag taaaaatcaa aagccttctg tagacccggt cattattgtg
     2161 atgcttggcg taattttcct tgccgtggct attgccatga tcgtgtttca gcctactagc
     2221 aattccatca acctcactgc cgatgccagt gtgatgagcc aacttgtagt ggcattcgtt
     2281 accggcatta ctacgggcgg gttgagctgc cttgcagtgc aaggcggttt gcttgccagt
     2341 tcacttgcct atcagatcga gcaggattac agcgcacaag ccaaaaataa aaaagccacg
     2401 cgtacaaatt ctgccatgcc gatcgcactc tttcttattg ccaaactcgt tgcatatact
     2461 ttgatggggg cgttgctcgg ctggctgggt tctttcctca ccttaagtcc gctcacgcgc
     2521 gccatcctga tgatcgccat cggcattttc atggtgggta acgcgttgcg catgttcaac
     2581 gtccacccga tctttcgtta cttttccatt gagccgccga agttcatcac acgatatatc
     2641 cgccgaactg ccaaaggcac agataccgcc acacccatct tcctcggctt gctcacgatc
     2701 ttcattcctt gtggcgtcac tcaagcgatg atggcaaccg ctctcggcac agggagtgta
     2761 cttatgggtg cagcattgat gttcgccttc acccttggca ccagcccggt atttttcatc
     2821 gttgcatatc tcgccacaga gctcggcgca aaacttgaaa aattcttcat gcgatttgtc
     2881 gccgtggtgg ttttgatctt gggactcgtc acccttgatg gtggactcaa cttaatgggc
     2941 tcgcccctct cgcttcaaaa catgactcgc ggctggttcg ccccacaagc agaatccaac
     3001 gctataacag aatccgtcca gccctctgct gcaagtgatg aaattatcct aagcgtagag
     3061 aacaacgggt atttccctgc cattctcaaa gctccagcag gcaaagacct gaccttaagc
     3121 ctcgtcacga ataaaaccta ttcctgtgcc cgcgactttg ttattcccga tttaaatttc
     3181 tacgaattgc ttcccgatac aggtactgtt caagtgaaca tcccagcgca gaaagccggc
     3241 tcaaccttgt tcttcacttg ctccatgggt atgtacaccg ggcagattgt gttcgaatga
     3301 taaacgtagg gtcgccccta cattggagat gaaatgatta agaaaacctt caaaatcccc
     3361 gatatgagtt gtaccaactg cgccatgaag atcgagtctc tggaagatga cctggatggc
     3421 gtgaaagagg tcaacgccag ctatcacaaa ttggagatgg tcatcgagta cgatgaggcg
     3481 aaactaacgg atgagcaaat cgtgaccgct gtaaaaaaga agggctatac cgcagtcgcc
     3541 gcataagttg accttttaaa aggcattatc aaggcatgtg gagcaataaa tgtgcgcaac
     3601 ccatttaagc tccacatgcc tacttaattt aaagattttg tttgttttca ttttgacccc
     3661 tttccgttat actcgcgccc atgcccaagt attacacgcc caaagaagcc aataatctgt
     3721 tggaaatcgt ccgaccaatg gtaggagatt tgatgcagat cggggaacgg gtccgcgctc
     3781 gtggacctga gatttgggcg atggttgaaa aatctgcggg gaacggcggc aaccccgagc
     3841 taagcaagat gctgcccgat tttgaccgtc tcgatgagat cctgcatcgc cttcaggata
     3901 tggagatcga ggtgaaagac ctggcttcag gcttgatcga ttttccagcg ttgcgagatg
     3961 gcacggtcgt ttatctgtgt tggaaatatg gagaggggag tatccagttt tggcacgaga
     4021 tcgaggcggg gtttgcgggg cgtcaaccga ttgattggga ataatccgaa cgctaaaaat
     4081 tcattgtatc ccggagagaa gagatgagtg catccacaat tggacgttat gagatcgaat
     4141 ctgaattagc cacagggggc atgggggtta tcatccttgc gaaagaccca tacattcaga
     4201 gacaggtcgt tgtcaaagtg ttgatgtata gtcgcacctt ggatacggtc taccgcgatt
     4261 ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgtca aagtgttgat
     4381 gtatagtcgc accttggata cggtctaccg cgattttttc cagaatgaag cggaagtcat
     4441 tgctgcgttg gaacatccct gcattgtgcc catttatgat tttggctggc atggacaaca
     4501 accgtatatc gtgatgcgtt atatggcggg cggcagttta caagaccacc ttgaaaaagg
     4561 cgagatcaaa ctgacggaaa tggcgcatat ctttaagcgt gtggcagatg cgcttgatgc
     4621 tgcacatgca aagtccatta ttcatcgcga tatcaagcca tccaacattt tgttcgatgc
     4681 ctctggtgaa gccttcctct ctgattttgg gattgcaaca tcaaaatcca tcaaggatga
     4741 tgatggcgaa tggttggtgg gcacacctgc atatatgagc cctgaacaag ccaaaggtgg
     4801 gcatgtagat ggacgttcag atgtctatgc attaggtgta acgttgtatc gccttctgac
     4861 cggacagttg ccattctcca gtgattcagc gactagcctg attaactctc acgcagagct
     4921 accgatccct gatattcgca gtgttaaatc aaatattcct gctgtgtggc aagaagtcgt
     4981 ttccaaagcc atggcgaaag accccaaaga tcgttatgcc accgctggcg attttgctcg
     5041 agatgtgaac gaagtcgtct ctggaaaatg gttcttgagg aaattgtagg aaaatttctt
     5101 tcaaacgcat ctacaagaaa ataaagtgtt tttctttata agagcctgtt aaggctctat
     5161 attgttttac gttaggagaa aaatgaaaaa aggtatttgg tacggcatcg cagcatatgc
     5221 gatgtggggt ttcttcccta tctattggaa attgttacat gatattcccg ctttgcaatt
     5281 gcttgggcat cgtatttctt ggtcattcct gctcttattc gcattcattt tcatcaccaa
     5341 acaatgggac gaattccgcg ctgttgcatt caaccgcaga acccttggca tctatgccgt
     5401 agcaggtgtt ttgctttctc taaactggtt gatctacgtc tggggtgtga acgctggctt
     5461 cattgttgaa accagccttg ggtatttcat caacccgcta ttaagcgtgc tctttggcgt
     5521 gatcttcttg cgcgaaaaac tacgtcccat gcaatggttg ccggttgtga ttgctaccat
     5581 cggtgtcacc tatctcacca tcacatacgg acgcttgccc tggattgcgc tttcgttggc
     5641 ggttagcttt ggtttgtatg gcttggtcaa gaaactttca ccgctcggtt cggtcttcgg
     5701 gttgactctc gaaacaggca ttgtcttccc tgttgccttg atctaccttg cttttgttca
     5761 agtgaacggc actgctggct tcttgcatga tggcgctctc gttgatattc ttttgatcgg
     5821 cggcggcatt gtcaccacca ttccgctttt gatgtttgcc tctgcggcga aagaaattcc
     5881 gctcaacatg gtgggtgtgc tgcaatattt tgcaccgacc attcagtttc tcatcggcgt
     5941 ctttgtatac aaagaacctt tcgaccatac tcgtttgatc ggctttggca ttgtttggtt
     6001 ggcgctcatc atctttgggc ttgagaatat tcttgctcat cgtaccccgc ctcaaaccat
     6061 ccctgagttg ggcgaataaa aatgaagctc ctttttattg gcggcacggg gttgatcagt
     6121 tcggcatgct ctgaactcgc cattgaacgc ggacacgaac tttttctttt gaatcgttct
     6181 gcctcgaaga tctacccgcc gccgaaagga gctactgttc tacaggcaga tatctacgct
     6241 gaccctgcgc atctttccac gctcttggcg ggtcagcgtt tcgacgccat agtggacttc
     6301 attgcttttc atccctatga tattcaacgc gacttatctt tattccgcga taagacagac
     6361 caatttgtat ttatttcttc tgccagtgcc tatcaaaagc cggtacggaa atatcccatt
     6421 accgaagaga cgccgctgga gaatcctttc tgggaatatt cgcgcaacaa gatcgccgct
     6481 gaaaatcttt tgatggacga atatcataag aacggctttc ctgtcactat tgtccgtcct
     6541 tcgcacactt atggaccttc acaaattccg ttcagttatg gcagttggcg tcatcagtgg
     6601 actttgattc accgcatgaa gcaaggtcaa cccgttatta tgcccggcga tggaacctct
     6661 ctgtgggtgt tgacatggaa tgctgatttc gccaaaggtc ttcttggctt gcttgggaat
     6721 aagaaggcga ttggtgaagc ctttcatatt acgtcagacg aagctctcag ctgggaccaa
     6781 atttattttg aaatgtatca agccttgggc gcggacccga acgtgattca catcccatca
     6841 gactatattg cccgcttcga ccccgaagcg accggaacac tgatcggcga taaatccaat
     6901 agtgtgattt ttgataatag taagatcaaa cgttttgtgc ctgattacca ttgtgaagtt
     6961 acatggcgtg aagggtttcg ccgttcgttg gcgtggtttg aggcacaccc tgaatttcag
     7021 accatcgatg aagatgccct gcaaaactgg gatcggatca ttcaagctta tacccgttaa
     7081 ccgcttataa cactggggga ggtgaggaga acaggtttca tgtcattcgg tcataaatca
     7141 gatgactttt attctaataa atatggtctg aattcgttat aattttgaga aaaaaacaag
     7201 gagagcaatt gtcatgaatt ttgcaatgtg gaagaaagca cttaatgtga tccctgaggt
     7261 ctcaaaagaa gaatgggacg gtctggatat catttccaaa tggctcattt ccacccgtgc
     7321 ggctgtgctc atcatgacct ttatttctgc tgccctggcg ggtttgttcg cctggcgtga
     7381 tggttcattt agttttctcc cctggcttgc gttggtcttc gggctcatca tggcgcatgc
     7441 cagcaacaat atcttcaacg actataccga ctttgtccgc ggtgtggata aggataatta
     7501 ctatcgcacc atgtatggag ctcaacccgt cgcttctggc ttgatgacca aacgccagca
     7561 cctgacctac tttgtggtca ccggcattct tgctatggtt gcgggcttat acctcgtctg
     7621 gcagaccgag ttcaacacca cgacatggat tttgctcgga cttggttcct tctttgtcct
     7681 cttctatacc tggcccctca aaggtttggc tctcggtgaa gttgccgtcc tcatggtctg
     7741 gggtccactc atgatcggcg gcggatacta cgtccttacg ggcaattgga actggtcggt
     7801 cgtcatcgcc agcctgcctt atgtgcttgg cgttaccacc gtcatcttcg gcaaacacat
     7861 cgataagatc aaagtggatc aagccaagaa gatctacacc ttgccggtag tcattggcga
     7921 aaaagcggct cgttatacga tcattgccat gatggtcttg ccctatcttt tcaccggcta
     7981 tctgatcgcc accaaattct ttacgccggt catgttgatc gtgctttttg cgctgcctgc
     8041 tttgcgcgag agccttccgc ctctcaccaa gcccaagccc gaaactcgcc ctgaaggttt
     8101 ccctgaagga cagggtggct ggcctcttta tttcgcgccc attggcttcc gcaacaaccg
     8161 cgcctttggc t
//
LOCUS       DAOREF010000006         7990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4436_length_7990_cov_4.589036, whole genome shotgun sequence.
ACCESSION   DAOREF010000006 DAOREF010000000
VERSION     DAOREF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_7990_cov_4.589036"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1072
                     /locus_tag="PKI33_00255"
     CDS             <1..>1072
                     /locus_tag="PKI33_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35454.1"
                     /translation="LWKILRALYPEHPKPALWASLFFLVFPGMGQQFIAFMYSHFYIV
                     LSALLLSMYLAIRAIQDEKRRWLYFSISYVLAFVNFITMEYFYFIEFGLLIVFYQLLP
                     GTFKERVKKTLLYFLPYLAIFIGITVWRTFFFEFQNASYQFVLLDKLKADIFSGLLYF
                     LNQILVSFWRTVFEAWILPFLTIGTTGFGPLTLSLMLVLLFSNILLTGVYLFAFKAES
                     SNGLDFAKRAGLVGLAFWGLSGGSVWMIGNLPQFNFSLDRFMLPFMLGSSILLASLIA
                     LIKSPRWQMILVALLIGFAGSRHFRLEEIYRSDWLTQQELFTQMTERIPGLKKGTILI
                     SNDIPVTYYSDNSLTGPLNWIY"
     assembly_gap    1073..1172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1173..1564
                     /locus_tag="PKI33_00260"
     CDS             <1173..1564
                     /locus_tag="PKI33_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35455.1"
                     /translation="VILFEKQNQLPGQLYGGAPEADWCHYFEQAELARQQGDWETVTH
                     LGDAAFSLSDYPNDPLERFVYIEGYAHQSNWKKAVELSKETYKISKNYVGPLLCRLWG
                     RIERETEASSEQVAALELVRSEFGCLP"
     gene            1697..2968
                     /locus_tag="PKI33_00265"
     CDS             1697..2968
                     /locus_tag="PKI33_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNM35456.1"
                     /translation="MRNLFARLAVLALALMLVLSACGGQPAATEEAPAATDAAPAATD
                     APAATDAAPVAEPLTFGMLLVGPYNDNGWSQAHYEAGKYVEEKLGAKMLYLDKVNPAD
                     RPGTTPDQLAEELVAQGATLIIFNSDDMKDASTTFAKNHPDVYVIMASGDQVWSDGKA
                     FEAMPNLTNIMGRMEYGKMMAGCAAALASETGKIGYLGPLINDETRRLAASAYLGAKY
                     CYEKAGNDPASLEFKVTWIGFWFNIPGVTLDPVQVSNDFYTTDYDVVISGIDNPVNLG
                     EAAKLKAEGKNVSGVAYDYVAACEAAPDVCIGVPYFNWGPEYVKAISSAQDGSWQSQF
                     IWAGPDWADINNPDTSTVGFVKGPALADDDAATLDAFIAELAGGLNLWSGPINLQDGS
                     AYLADGEVATDQQIWYLPQLLEGMDGLSAAE"
     gene            3045..4538
                     /locus_tag="PKI33_00270"
     CDS             3045..4538
                     /locus_tag="PKI33_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM35457.1"
                     /translation="MKVELKNIHKHFGKTYANKGINLNIPSGSIQGILGENGAGKSTL
                     MKILSGFIQADPGGEIVLDGKPAVMQSPADAIRFGVGMLHQDPLDFPPMKLIDNFLLG
                     MSGKLFPNRKETVQDFNLLAKEYGFNFDPENYVDSLTVGERQQLEIMRLLFLGARVFI
                     LDEPTTGISAAQKIKLFDTLRTFPEQAITTIFVSHKLEDVESLCDRVAVLRAGELVGE
                     FTPPYETKKFVSTMFGKEITLGERQPAKTGDVVLSLEDVSLEDSRVRIDHVNVDIRAG
                     EVIGLAGLEGSGQGMFIRACAGLVRPVGGKVMLKGKNLTGKPYHTFKHEGVNFLPAAR
                     LEEGLVPGLSLTDHFVLAEEPKGVFIDKAYGQKLAEDRIKSFNIRGTPASMIESLSGG
                     NQQRAELALLQTPLSLVLLEHPTRGLDIESVIYIWSKLKDRCKQEGAAIIFISSDLEE
                     VLQYSDRLLVFFSGKVSEPIDAATTSVEQLGQLIGGKGWTKKGDIHA"
     gene            4531..5568
                     /locus_tag="PKI33_00275"
     CDS             4531..5568
                     /locus_tag="PKI33_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35458.1"
                     /translation="MLKTKAGGFIFQLIAFLLAMGFVSLILLAVGASPIEAYKNMLTG
                     SVGSMRKFAEVLVSFVPLLLVTAGLLITFSAGLWNIGVEGQIVLGAIGSVWVLRQFVD
                     STVSPAIVMTLAILGGMVAGALWASLAGALKTFGGVNEIFGGLGLNFVSTALTLYLIF
                     GPWKREGVASMSGTEPFPDAFSLPQLPDLRISPYALVIALIAIVIVYFILQGTYFGLK
                     IKAVGKNIRAAYLLGIPTWQYSLYSFLICGALAGIAGSLQVTAVYHRLIPSISSGYGY
                     LGLMVAMLVNYQAIWALPVALFFAALNIGSIQLPIVMQLDSSLSGVLQGVLVLFVLLT
                     EGARQKFGKKG"
     gene            5576..6574
                     /locus_tag="PKI33_00280"
     CDS             5576..6574
                     /locus_tag="PKI33_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35459.1"
                     /translation="MNQETLLVGLAGVLAAAAPIIFAVMGETLSERAGVINLSMNGTI
                     LLTAMSGFAVAYKTDSLILGFLAGMLIGALVALVVAFASITLKQSQVAIGFVLALTCR
                     DLAYFLGNPFMGVTGPRLQATPIPGLSDIPILGPVFFRQEVITYVSFLAIFIAWLWIY
                     RTRPGLMLQGIGEKPAAAYVRGANVNLMRYVYTVLGGALIGLAGPTYSLGVRAGWMGT
                     ISGLDGIGWIALSITIFGGWNPARAAFGSYLFAFLQWLGLVLQPVLTQVPSQVLQVAP
                     FPLMILTLLLVNIGNAEWVERTLAAMPESARKFTAKLLRAMRASPPAALGVPFERD"
     gene            6653..7921
                     /gene="thrC"
                     /locus_tag="PKI33_00285"
     CDS             6653..7921
                     /gene="thrC"
                     /locus_tag="PKI33_00285"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNM35460.1"
                     /translation="MNKFNGYRCSICGSEYLPGQVTYTCPKDGGNLDVVLDVDTIKKK
                     FQPEDLISRTEASLWRYLPLFPVNEPVGDSTPLHASGWTPVFALPRLAEKLHLKHLWL
                     KDESRNPTASFKDRASALVVTRARELKSEIVVTASTGNAGAALAGMAAAVGQKAVIFA
                     PKTAPQAKVAQLLVFGAKVILVDGTYDDAFDLTVKASNEFGWYCRNTGYNPFTLEGKK
                     TAAFEIWEWWIEAHRDWHKKDSTLDNHSPLTIFVSVGDGNIISGIHKGFKDLLALGWI
                     SNMPRIIGVQAEGSAAIANAFHAGTETITPVSATTIADSISVDLPRDGVRAVRAAQQT
                     DGTYIVVKDEDIIKAIAELGKIGVFAEPAGATAYAGLVKATSQGVVGSDDPVLVLNTG
                     SGLKDVRAALQAVQSAPIIEPTLEAVKKIL"
BASE COUNT         1782 a         1805 c         2037 g         2266 t
ORIGIN      
        1 tgctttggaa aattttacgc gcactttatc ctgaacatcc caaacctgct ttgtgggcaa
       61 gtttgttctt ccttgtattc cccggcatgg ggcagcagtt catcgcattc atgtacagcc
      121 atttttatat tgtgctgtct gctttgttgc tttccatgta tcttgcaatt cgcgccattc
      181 aagatgaaaa aagacgctgg ctttattttt caatatcgta tgtgctggct ttcgtcaatt
      241 tcattacgat ggagtatttt tacttcattg aatttggttt gctgattgtt ttttaccagc
      301 tcttgccggg cacgttcaaa gaacgtgtaa aaaagacgct tctttatttt ctcccttatc
      361 ttgccatctt tattggcatc acggtttggc gcactttctt tttcgaattt caaaatgcga
      421 gttatcaatt tgttttgctc gataaactaa aagcggatat tttctctgga ctgctttatt
      481 tcttaaatca aattctggtc agtttttggc gcaccgtgtt tgaggcgtgg attttgcctt
      541 tcctcaccat tggcaccaca ggctttggtc cgctcacact gtctttgatg ctggttctgt
      601 tattctcaaa cattctcctc actggagtgt atctcttcgc gttcaaagca gagtcttcaa
      661 acggtttgga ttttgccaaa cgagcaggct tggtcggctt ggcgttttgg ggcttatctg
      721 gcggctctgt gtggatgatc ggcaatttac cgcagttcaa tttttcgcta gaccgtttca
      781 tgttgccgtt tatgctaggc tctagcattt tgttggcaag cttgatcgcg ttgatcaaaa
      841 gtccgcgctg gcagatgatt ttagtggcgc tgttaattgg ctttgcaggt agtagacatt
      901 ttcggttgga agagatctat cgtagtgatt ggttgaccca acaggaattg tttacacaaa
      961 tgacggaacg aatccctggg ttgaaaaaag gaactatctt aatttctaat gacattcctg
     1021 tgacatatta ctctgataat tcactcacag gaccattaaa ctggatttat agnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgtgatt ctgttcgaga aacagaatca
     1201 gttaccaggt cagctttacg gaggcgcgcc ggaggcggat tggtgtcatt attttgagca
     1261 ggctgagctg gcgcgtcaac agggcgattg ggaaactgtt acccatttgg gcgatgcggc
     1321 gttttccttg agcgattatc ctaacgaccc gctggaaagg tttgtatata tagaaggata
     1381 tgctcatcaa tcgaactgga aaaaggcggt agaattatcc aaagaaacct acaagatttc
     1441 taagaactat gtaggtcctt tgttatgccg tttgtggggg cggattgagc gggaaacgga
     1501 agcttcttcg gagcaggttg cggctttgga gttggttcga agtgagtttg ggtgcttacc
     1561 ttaaaaatac gttgtacctt tcttcgcaca tagaaagttt agtaaaattg tttagacaac
     1621 tgaaaggagg ttcgaaagaa gatttcgttc ttggtcagtc gtcaaacaga aaaaaatttc
     1681 aataaggaga agaagtatgc gtaatttatt tgctcgcctc gccgttttgg cgttggcact
     1741 aatgctcgtt ttgtctgcct gtggtggaca acccgcagcg accgaggagg ctcccgccgc
     1801 cacggacgct gctcctgccg caacagacgc acccgctgct accgatgctg cccctgttgc
     1861 tgagcccctt accttcggta tgttgctcgt tggtccgtac aacgacaatg gctggagcca
     1921 ggctcactat gaagctggca agtacgtcga agagaagctc ggcgccaaga tgctctacct
     1981 cgacaaagtt aatcccgctg atcgccccgg caccaccccc gaccaactcg ctgaagaatt
     2041 ggtggcacag ggcgctaccc tcatcatctt caactctgat gatatgaagg atgcctccac
     2101 caccttcgcc aagaatcacc ctgatgtgta cgtcatcatg gcttctggtg accaggtttg
     2161 gtctgatggc aaggcgtttg aagccatgcc caacctcacc aatatcatgg gacgcatgga
     2221 atacggcaag atgatggctg gttgtgccgc tgctctcgct tctgagaccg gtaagatcgg
     2281 ttacctcgga ccgctaatca acgatgaaac ccgccgcctc gctgcctctg cctacctcgg
     2341 cgcgaaatac tgctatgaga aggctggcaa tgatcctgct tccttggaat tcaaggtgac
     2401 ctggattggc ttctggttca atatccccgg cgtgaccctc gaccccgttc aggtgtctaa
     2461 cgatttctac accaccgatt acgatgtggt gatctccggt atcgataacc ctgtcaacct
     2521 tggtgaagct gcaaaactca aggctgaagg caagaatgtc tctggtgtgg catatgacta
     2581 tgtcgccgct tgtgaagctg ctcctgatgt ttgtatcggt gttccgtact tcaactgggg
     2641 tcctgaatat gtgaaggcga tctcctccgc tcaagatggt tcctggcagt ctcagttcat
     2701 ttgggctgga cccgattggg ctgacatcaa caacccagat acctccacgg ttggtttcgt
     2761 gaagggacct gccttggctg acgatgatgc tgctaccctc gatgcattca ttgccgaact
     2821 cgctggcgga ctgaaccttt ggtctggtcc gatcaacctg caagatggaa gcgcctacct
     2881 cgccgacggt gaagttgcta cagaccagca gatctggtac ctgccgcaat tgctcgaagg
     2941 tatggatggc ttgagcgcag ccgaataatg tagttccctt tgtaggggcg cggacaccgc
     3001 gcccctacat tttcatttca ccctcatgga ggacccgcac caccatgaaa gtcgaactca
     3061 agaacattca caagcacttc ggcaagacct atgccaataa gggaattaat cttaatattc
     3121 cttctggctc gatacagggt attttggggg agaacggcgc agggaaaagc accctgatga
     3181 agatcttatc cgggttcatt caagcggacc caggtggtga gatcgtttta gacggaaaac
     3241 ctgcagtgat gcaatctcca gcagatgcca tccgatttgg ggtggggatg ttgcatcagg
     3301 acccgctcga ttttcctccc atgaaattga tcgataactt tttgcttggc atgtcgggaa
     3361 aacttttccc gaatagaaaa gaaaccgtac aggactttaa cctgctggca aaagaatatg
     3421 gttttaattt tgaccccgaa aattacgtag attctctaac ggttggtgaa cgccaacagt
     3481 tggagatcat gcgtttatta ttccttggtg cgcgtgtgtt cattttggat gagccgacga
     3541 cgggaattag tgctgcgcaa aagataaaat tgtttgatac gcttcgaaca tttccagaac
     3601 aagccattac aacaattttt gtgtcacata aattggaaga tgttgaaagc ttgtgcgacc
     3661 gtgtcgccgt gttgcgagca ggagaactgg ttggtgaatt tacaccgcct tatgaaacca
     3721 agaaatttgt ttcaaccatg tttggcaaag agattacttt aggtgaacgt caacctgcca
     3781 aaacgggcga tgttgtgctt tcgttggaag atgtctcttt ggaagattcc cgtgttcgca
     3841 tcgatcatgt gaatgtcgat attcgtgctg gagaagtgat tggtcttgct ggtttggaag
     3901 gttctgggca aggcatgttt attcgtgcat gtgctgggct cgtccgcccg gtgggtggca
     3961 aggttatgct taaaggcaag aatttgacgg ggaagccgta tcacactttc aagcatgaag
     4021 gtgtcaactt ccttcccgcc gcaagattgg aagaaggctt ggtgcccggc ttgagcctga
     4081 ccgaccactt cgtccttgcg gaagagccga agggcgtgtt cattgataag gcatatggtc
     4141 aaaaactggc ggaagatcgc atcaaatcct tcaacattcg cggtacgcct gccagtatga
     4201 tcgagtcgct ttcgggtgga aatcaacagc gggcagaatt ggcgctttta cagacgccac
     4261 tatcgctggt cttattggag cacccaaccc gcggcttgga tattgagtcg gtgatctata
     4321 tttggagcaa actcaaagat cgctgcaaac aagagggcgc agccatcatc tttatttcgt
     4381 ctgacttgga agaagtttta cagtacagtg accgtttgct agtgttcttt agcggtaagg
     4441 tttcagaacc gatcgatgct gcgacaacca gtgtggaaca actaggtcaa ttgatcggcg
     4501 gtaagggttg gacgaaaaaa ggagacatcc atgcttaaga ccaaagcggg tgggtttatt
     4561 tttcaattaa tcgcgttcct gcttgctatg ggatttgtct cgttaattct gttggcagta
     4621 ggcgcttctc ctatcgaagc gtataaaaat atgctgaccg gctcggttgg ctctatgcgt
     4681 aaattcgccg aagtgttggt gtcatttgtg ccattgcttc tggtaacggc tggtttgctt
     4741 attaccttct ctgccgggct ttggaatatc ggtgtggaag gtcagattgt tttaggtgcc
     4801 attggctcgg tttgggtctt gcgccaattc gtagatagta cggtttctcc tgctattgtg
     4861 atgactctgg cgattttagg cggaatggtt gctggtgcct tgtgggcatc gttggcgggc
     4921 gctttgaaaa cctttggcgg tgtgaatgaa atttttggtg gcttggggct gaactttgtc
     4981 tccacggcat tgaccttgta cctgatcttc ggtccatgga agcgtgaagg ggtggcgtcc
     5041 atgagcggaa ccgaaccgtt ccctgatgca ttctctttgc cgcaattgcc agatttacgc
     5101 attagcccgt atgctttagt gattgcattg atcgcaattg ttattgtgta tttcatctta
     5161 caaggtactt actttggttt gaagatcaaa gccgtaggta agaacatccg cgctgcgtat
     5221 ttacttggta ttcctacctg gcaatattct ttatactctt tcctcatctg tggagccttg
     5281 gcgggcatcg ctggttcttt gcaagtgact gcggtgtatc accgcctaat tccttccatt
     5341 tctagcggat acgggtatct tggtttgatg gtggctatgc ttgttaatta ccaagccatc
     5401 tgggcgttgc cagtcgctct tttctttgcg gcgttgaaca ttggtagcat tcaattgcca
     5461 attgtgatgc aattggattc gtctctctca ggtgtgctgc agggtgtttt ggtgctcttc
     5521 gttttgctca ctgaaggggc tcgccaaaaa tttgggaaga agggataagg catccatgaa
     5581 tcaagagact cttcttgttg gcttggcggg cgtacttgct gcagcggctc ccattatttt
     5641 tgcggtgatg ggcgagaccc tttctgaacg agctggcgtc atcaatttat ccatgaacgg
     5701 aaccattctg ttgactgcta tgagcggatt tgcagtcgct tataaaacag atagcttgat
     5761 tcttggtttt cttgctggca tgttgatcgg tgcgcttgtt gctttagtgg ttgcatttgc
     5821 cagcattacc ctcaaacaat ctcaagtggc aattggcttt gttttggcgc tcacatgccg
     5881 cgacttggca tactttctcg gcaacccatt tatgggtgtt acaggcccgc gtttgcaagc
     5941 gacacccatt cctgggctga gcgacattcc gattctcggt cctgtatttt tccggcagga
     6001 agtgattacc tatgtcagct tcttggcaat cttcattgcc tggttatgga tttatcgcac
     6061 gcgccccggt ctaatgctac aaggcattgg cgaaaaacca gcagccgcct atgtgcgtgg
     6121 cgcgaatgtg aatctaatgc gctatgttta tacagttctg ggcggtgctt tgatcggttt
     6181 agcaggaccc acgtattcgt tgggcgtccg cgctggatgg atgggaacca tttccggctt
     6241 agatggaatc gggtggatcg ccctttccat cacgatcttt ggtggttgga atccggctcg
     6301 cgctgcgttt ggttcatacc tcttcgcttt cttgcaatgg cttgggctgg ttttgcaacc
     6361 ggtgcttacc caagtccctt cgcaagtctt gcaggttgcg cccttcccgt tgatgatcct
     6421 cactctcttg ctcgtcaaca tcggcaatgc ggaatgggtc gagcgcacat tggcagccat
     6481 gccagaatct gcgcgaaaat ttacggcgaa gttattacga gccatgcggg cttcaccgcc
     6541 agctgctttg ggcgtcccgt tcgagcgcga ttagttgcaa cataaaagga ctctcgtatg
     6601 agggtccttt ttgattttta tatcgggtaa aattaagccc tagaggaaat gtatgaataa
     6661 attcaacggg tatcgttgtt caatttgtgg gagtgagtat cttccagggc aggtcacata
     6721 tacgtgtcca aaagatggcg ggaacttgga tgtggtctta gacgttgata ccatcaagaa
     6781 gaaatttcag cctgaagatc tcatctctcg aacggaggct tcgctgtgga ggtatttacc
     6841 actgttcccg gtcaatgaac ccgtaggcga ttccacgcct cttcacgcct ccggctggac
     6901 cccggtcttt gccctgccac gattggcaga aaaacttcac ctgaagcatc tctggctcaa
     6961 agatgagtcg cgcaacccta ccgcctcgtt caaagataga gctagcgcct tggttgtgac
     7021 acgtgcccgc gagctcaaat cggagatcgt agtcactgct tcaacgggca acgccggagc
     7081 agcattagca ggcatggctg ccgctgttgg acaaaaagcc gttatctttg cacccaaaac
     7141 tgctccacaa gcgaaggttg cgcaattgct ggtctttggt gcaaaagtca ttctggtaga
     7201 tggaacctat gatgacgcct ttgacctgac cgttaaagcc tcaaatgaat ttggttggta
     7261 ctgtcgtaat acaggctata acccgttcac actggaaggt aagaagaccg ccgcttttga
     7321 gatctgggaa tggtggattg aagctcatcg tgattggcac aagaaagata gtaccttgga
     7381 taatcattct ccgttaacta tctttgtctc tgtaggggac gggaatatta tctctggcat
     7441 tcacaaaggc ttcaaagatt tattggcact cggctggatt tcaaatatgc ctcgcattat
     7501 tggtgtgcaa gccgaagggt ctgcggcaat tgctaatgcc ttccatgcag ggacagaaac
     7561 catcacaccc gtgtctgcca caaccattgc agatagcatc tcggtcgatt tgccgcgaga
     7621 cggtgtccgt gcagtgcggg cagctcagca gaccgatggc acgtatattg ttgtcaaaga
     7681 tgaggatatt atcaaagcca ttgcagagct gggaaagatc ggcgtctttg ccgaacctgc
     7741 tggggcgacg gcatatgccg gtttggtcaa agccacaagc cagggcgtgg ttggaagcga
     7801 tgatcctgtt ctcgtcctga atacaggcag tggactcaaa gatgtgcgag cggcattgca
     7861 ggcagtacag tccgcgccga ttattgaacc aactttagaa gcggtgaaaa agattttatg
     7921 aaaaaaacta acgatcagtg gagtcttccc tttcggtata cgatggggat catcattttt
     7981 atcctcgttg
//
LOCUS       DAOREF010000007         7962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4470_length_7962_cov_4.653851, whole genome shotgun sequence.
ACCESSION   DAOREF010000007 DAOREF010000000
VERSION     DAOREF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_7962_cov_4.653851"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..500
                     /locus_tag="PKI33_00290"
     CDS             276..500
                     /locus_tag="PKI33_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35461.1"
                     /translation="MNKIDKRNVLDNEVFTYKINKEKKVFIYWRGKQVLILRGSEAQK
                     FTSKIIGLDDKGVQLVLAKLTGNFKRGNER"
     gene            582..2891
                     /locus_tag="PKI33_00295"
     CDS             582..2891
                     /locus_tag="PKI33_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM35462.1"
                     /translation="MPGREDIFQKAMNEGHSAAWDQEWSKAVAAYRRALEESPDNPKA
                     LSSLGQALHQLNNVEEALQIYMRAVKVSPDDPVSMEKVAQLSERVGDLKTATEAAIRA
                     GDLFLKQRDTDKALENWVHVTSINPEHAIAHSRLAQVHERLGHGQQAAMEYLAIASIL
                     QRAGNPEKTQEMVNKAQSLVPNSAEVKQAQTLLRTGQLLPKPIRAKGGTGPIRMAQVK
                     QLQEPAKKAASGLDPIAEARQKALTQLAEVLFDLSDDSSPVAQDRKGISAIMRGTGSL
                     SAQSEQLKVVLHLGQAIDAQSKGNDTLAAEEMEAALNAGFSSSALSFSIGYLRFKNER
                     YESAQRALQNSVKHEDYGLASRLLLGDIISRKGDFRDAATQYLEALKFADAATVPLEQ
                     GDDIRQQYEPLIESYQQQTDEAVLRKVCDNIRGLLMRADWRDQLHKTRDQMPKQDGMA
                     APLADMILQVQSSSVLESMNRINQLARMGSLRSAMDEAYDAIQHAPTYLPLHIIMGDL
                     LVQEGHTTDAIAKFSVVAHSYSVRGEVMQASKLLRRIIQISPMDLGARNRLIDQLVAR
                     GQVDDAIQEYLELAAIYYRLAELDMARKTYTHALRIVQQGNASRDWNTHILQRMADID
                     MQRLDWKQALRVYEQIRTLMPDDDASRKQLIELNMRMGQQDRAMSELESYITHLESQN
                     KQELAIAFLEELARDHEDQPLIKRTLAALLHRSGRIGEAIPILDQLGEALLEKGDKQG
                     AMEVINQIVLMNPPNVQDYRVLLSQMQNS"
     gene            3033..4067
                     /locus_tag="PKI33_00300"
     CDS             3033..4067
                     /locus_tag="PKI33_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNM35463.1"
                     /translation="MPKTQNRAVMIWLYVFAGVVAFLVVFGGFTRLTRSGLSIVEWNP
                     INGTVPPMAQHQWEAEFEKYKATPEYQLINTDMTLQEYKYIFYVEWFHRLVARMAGLF
                     YALPVFYFLIRKTIPLKEFGIYFWMGVLFIGQAVMGWMMVYSGLVDRPAVSHFRLTAH
                     LLLALTLFSLAIWTATGHRLGFHHYEKKSKWSLPTKISVAALVVLAIQISYGGMTAGL
                     KAGHVSDTWPLMFGKLIPPNLFNSFINILETPQTIVFIHRWFAWLGVILVPIVIYIIK
                     KQNYPADIQKGLLWLLGVVALQITLGVLTILSYVNIVIALLHQANAILLLGLAVYFIH
                     RFRAVDEAKA"
     gene            complement(4112..4348)
                     /locus_tag="PKI33_00305"
     CDS             complement(4112..4348)
                     /locus_tag="PKI33_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35464.1"
                     /translation="MMIKNRFYMFLGLLLLLLGVVLPFLMIIKVVEPTFFLVFLTSGS
                     STVGLALGMFGLTRWSVTAIKKRPPQIEDWRQTE"
     gene            complement(4345..5376)
                     /locus_tag="PKI33_00310"
     CDS             complement(4345..5376)
                     /locus_tag="PKI33_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM35465.1"
                     /translation="MISELQEAKRMSVKIDEPTETLLAIHNLHVWYELRRFGFSVVGH
                     VKAVNNVSFELGKGETVAVVGESGCGKSSLMKTILGLNVPTGGELLFEGKRLTALTEA
                     ELRSYRFEIGYVQQDPYGALPPFMNVEQILEEPLIISGVSSKDERRERIHKILGEVKL
                     HPVEDFLHKYPHMLSGGQQQRVVIARAMVLEPKMIVADEPVSMLDASVRVEILKLMHA
                     LQEQHHLSVIYITHDLSTVKYFSARIFVMYAGSLIEKAETRQLLKNPLHPYTHALLAA
                     TSDPNAANAEVYKAVPPGEPPSLADPPKGCRFHPRCARAMAGVCDVKEPPDFEPEPNH
                     FVACWLYEA"
     gene            complement(5393..5971)
                     /locus_tag="PKI33_00315"
     CDS             complement(5393..5971)
                     /locus_tag="PKI33_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM35466.1"
                     /translation="MPSLALYDVLYSFKDIHKVVLEFASELNEEQLRWRPRGYSTSIG
                     FHLWHLARESDFLKAIILERTPQLGSDFGEPKEIWAVEKLAERWRFPTDLNATVGTGL
                     SDEIAATLPIPPKDELVDYLQKSYEALETFIALLDERYPTFEGVEEDLQRKLKNIRLN
                     LLVFLSHDCRHLGMMECLKGLQTGFGSATEKR"
     gene            complement(5989..6948)
                     /locus_tag="PKI33_00320"
     CDS             complement(5989..6948)
                     /locus_tag="PKI33_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM35467.1"
                     /translation="MTELTPLLEVKDLKLHFRTQKGVVQAVDGVDLDLGFHRAVVILG
                     ESGCGKTSLSKAMLRLLPRNVAKYSGELVINGKDVMPFTDEEYRQNVRWVSMSLVPQA
                     AMNALNPVITVGEQVSEPAMLHLGLNKDEALGMAYKMFQHVGIPADFISRYPFELSGG
                     MRQRVALAMALVTSPNLIILDEPTSALDLLTQANIMNVLKRIKHELGTSYILITHDIA
                     TSSELADEVAIMYAGQIVETGDAKDFFPAPLHPYSQMLMASVPRLRSESDPMFITGQP
                     PSLMNPPTGCRFAARCPFRFEKCDQEPPVFQKGNRKVKCWLHQ"
     gene            complement(6962..>7962)
                     /locus_tag="PKI33_00325"
     CDS             complement(6962..>7962)
                     /locus_tag="PKI33_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35468.1"
                     /translation="YPQDMIVYVNALFDQKQPFISMEWITPDGRTIRVSNFATAAKST
                     YRFSQDDKLAAKLRAEDPIPALFSDPDTGEVLKGPYKLVITGTTFEPNSNIDVELVVH
                     GQVYGLAGTDHARRDLTLPLLWGAPVALAFGLIAAIGTSVLTMVIAAVGTWYGGWVDQ
                     LVQRITEVNLVLPFFAIMIMIGTFYSRSIWTIISATIVLNIFTGAILGYRAIFLQVKE
                     SMYIEAAKAYGATNWRIIFHYLIPRMIPLLIPGLVQAVPAFVFLEASLAVIGLGDPVL
                     PTWGKIIQDAQANGALYKGYYYWVLEPAVLLIITGLAFAFLGFVLDRIFNPRLRDV"
BASE COUNT         2181 a         2089 c         1809 g         1883 t
ORIGIN      
        1 gcaggctaac aaagcatgcg gcaaagatgt tatctgcttt gctgtctcgg tgagcccagg
       61 cgcgggcttc gcaaaaaata cagcaggtat ataaaggatt ggtatgagtc cgaaaaatcc
      121 aaccgactgg cttttattct atattgtgac aggtcttatt ttatttttta taatatgtat
      181 aagcatattc ataatgctct ttcttgatgg agccttcctt tgggcatcct aatattctag
      241 tttgccatat agaatacaaa agatctttag acattatgaa caaaatagat aaacgaaacg
      301 tattggataa tgaggtcttt acctataaaa tcaacaaaga gaaaaaggtg tttatttact
      361 ggcgggggaa acaagtcctg attttacggg gaagcgaagc ccaaaaattt acgagcaaga
      421 tcatcgggct agacgataaa ggagtgcaac tggtgctggc aaagctgacg gggaacttca
      481 aacgcggcaa cgaacgctag cgcacgcccc taaccaataa ggttataatt gcatgattgt
      541 taggacgacc caccctattt tgaaagtaaa ataacaacac tatgcccgga cgagaagata
      601 tcttccaaaa agccatgaac gagggacact ccgctgcctg ggatcaggaa tggagcaaag
      661 cggttgccgc ctatcgccgc gccctcgaag aatcacccga taaccccaaa gcgctcagca
      721 gtctcgggca ggcactccat cagctcaaca acgtggaaga agccttgcag atctacatgc
      781 gtgccgtcaa ggtctcgcca gatgacccag tctccatgga aaaagtggcg caactctctg
      841 aacgcgttgg cgacctgaaa accgccaccg aagccgccat ccgcgcaggt gacctcttcc
      901 tcaaacaacg cgataccgac aaagccctcg aaaactgggt acatgtcacc agcattaacc
      961 ccgaacatgc catagcccat tcacgccttg cacaagtcca cgaacgtttg gggcatggtc
     1021 aacaagccgc catggaatat cttgccattg ccagcatttt acaacgcgca ggcaaccccg
     1081 aaaaaacgca agagatggtg aacaaagccc aatcgctggt acccaacagc gccgaagtga
     1141 aacaagccca aacgttatta agaaccggtc aactcttgcc caagccaatt cgcgccaaag
     1201 gcggcacggg acccatccgc atggcacaag tcaagcaatt gcaggaaccg gcgaagaaag
     1261 ccgcctcagg gctggacccg atcgcggaag cccgtcagaa agcgttgact cagctggcag
     1321 aagtcctctt cgatctctcc gacgacagta gccccgtggc gcaagaccgc aaaggcatct
     1381 ctgccatcat gcgcggaacc ggctcccttt cggcgcaatc ggaacaactc aaagtggttt
     1441 tgcatttggg tcaagccatt gatgcgcaat cgaaaggcaa cgacacgctt gccgccgaag
     1501 aaatggaagc cgcactcaat gctggcttta gcagttcagc tctttcattc agcatcggct
     1561 atttacgctt caaaaatgaa cgctacgaaa gcgcccaacg cgctttgcaa aattcggtta
     1621 agcatgaaga ttatggttta gcctcgcgcc tgttactagg cgatatcatt tcgcgcaaag
     1681 gcgacttccg cgatgcggca acgcaatatc tggaagcctt gaagtttgca gatgcggcta
     1741 ccgttccact agaacaaggc gatgacattc gccagcaata cgagccgttg atcgagtcgt
     1801 accaacagca aacagacgaa gccgttttgc gcaaagtctg cgataacatt cgcgggctgt
     1861 taatgcgtgc agattggcgc gaccagcttc acaagacccg cgaccaaatg cccaagcaag
     1921 atggcatggc tgcccctctg gcagatatga tcttgcaggt gcagtccagc tcggtgctcg
     1981 agtcgatgaa ccgcatcaac cagttggcac gcatgggcag tctgcgctct gccatggacg
     2041 aagcctatga cgccattcaa cacgccccca cctacctacc gcttcacatc atcatgggcg
     2101 atttgctcgt acaagaaggt cacaccaccg atgcgattgc caaatttagt gtggtagcac
     2161 attcatacag tgtacgcggc gaagtgatgc aagccagcaa gttattacgc cgtatcatcc
     2221 aaatctcgcc catggacctc ggcgcgcgca atcgtctgat cgatcagctt gtagcccgtg
     2281 gtcaggtcga tgatgctatt caagaatatc ttgaacttgc cgccatctac tatcgcctcg
     2341 ctgaacttga tatggcacgc aagacataca ctcacgcatt acgcattgtg caacaaggca
     2401 atgccagccg cgattggaat acccacatcc ttcagcgtat ggcagatatc gatatgcagc
     2461 gcttggactg gaaacaggct ttgcgcgtct atgaacagat ccgcaccctc atgcccgatg
     2521 acgatgcctc tcgcaaacaa ctgatcgaac tcaatatgcg catggggcaa caagaccgcg
     2581 ccatgagcga gctggaaagt tacatcactc accttgaaag ccaaaacaag caagaacttg
     2641 ccatcgcctt ccttgaagag ttggcacgtg accatgaaga ccaaccgttg atcaaacgca
     2701 cgctcgcagc gttgcttcac cgcagcggtc gcatcggaga agcgataccc attctcgacc
     2761 aattaggcga agccttgctt gaaaaagggg ataaacaagg cgccatggaa gtcatcaacc
     2821 agattgtgct gatgaaccca cccaacgtgc aagattacag ggtgctgctg agccaaatgc
     2881 aaaatagtta gccaaaagcg tggagttgct ctccacgctt ttttatacgc ttctcccggg
     2941 tgacaaatca cagcaacttt cgtgacaaaa gttctaacaa tctcctaagt cctgaatgct
     3001 ataatgccgc caattcaaat tggagtaatc atatgccaaa aacccaaaac cgcgccgtga
     3061 tgatctggct gtatgtcttt gccggggtcg tggcgtttct tgttgtcttc ggcgggttca
     3121 cccgcctcac ccgctctgga ctttccatcg tagagtggaa tcccatcaat ggcaccgtgc
     3181 cgcccatggc acagcatcaa tgggaagccg aatttgagaa atacaaagca acgcctgaat
     3241 atcaactcat caacacagat atgacgctac aagaatataa atacattttc tatgttgagt
     3301 ggttccatcg cctcgttgcg cgtatggcag gtcttttcta tgccttgccc gttttctact
     3361 tcctcattcg taaaactatt cccctcaaag aatttggcat ttacttctgg atgggcgttc
     3421 tcttcatcgg tcaagctgta atgggttgga tgatggtcta tagcggattg gtagaccgcc
     3481 ctgccgtcag tcacttccgc ctcacggctc acctcttgct tgctcttact ctcttcagcc
     3541 tcgccatctg gacggcgacc ggtcaccgac ttgggttcca tcactacgag aaaaagtcaa
     3601 aatggtcttt acctacaaaa atctctgttg ctgcgctggt tgtgctcgcc attcagatca
     3661 gctacggcgg catgacggct ggtctcaaag ctgggcatgt ctctgatacc tggccactca
     3721 tgttcggaaa actcattcca cccaacctct tcaactcatt catcaatatc cttgaaaccc
     3781 cgcaaaccat tgtctttatt caccgctggt tcgcctggtt gggcgttatt ctggtaccga
     3841 tagtgattta catcatcaag aaacagaatt acccagccga tattcaaaag ggtctgctct
     3901 ggctactcgg agtggttgcc ttacagatca cgcttggtgt tctaaccatt ctttcatacg
     3961 tcaacatcgt cattgccttg ttgcatcaag ccaatgcaat tctgctttta gggctggctg
     4021 tctacttcat ccaccgcttc cgcgccgtcg atgaagccaa agcataaata ctttttgtaa
     4081 aaatgcctcg ccgtaaatgg cgaggcattt tttattccgt ttgccgccag tcttctatct
     4141 gtggtggtct ctttttgata gccgtaacag accaacgcgt taacccaaac atgccaagcg
     4201 ccaagccaac ggtggatgag ccagaggtca agaaaacgag aaaaaacgtt ggttcgacca
     4261 cttttataat catcagaaac gggagcacaa cacccaacag aagcaaaagc aagcccaaga
     4321 acatgtagaa gcggtttttt atcatcatgc ctcatataac caacaagcca caaaatgatt
     4381 cggctcgggt tcaaaatctg gtggttcttt cacatcacaa acacctgcca ttgctctagc
     4441 acaacgcgga tgaaagcgac atcccttggg cgggtctgcc aaactgggcg gctcgccggg
     4501 cggaacggct ttatacacct cagcattggc agcattgggg tccgaggtag cggcaagcaa
     4561 ggcatgagta taggggtgca atggattttt tagcaattgg cgcgtctctg ctttttcaat
     4621 gaggctgcct gcatacatca caaaaatacg cgctgaaaaa tacttcactg tagaaaggtc
     4681 atgagtaatg taaatcactg aaaggtgatg ttgctcttgc aaagcatgca tgagcttcaa
     4741 gatttccacg cgcaccgaag catctaacat ggagacgggt tcatctgcca cgatcatctt
     4801 tggttccaac accatggctc gcgcaatcac cacgcgttgc tgctgcccgc cgctcaacat
     4861 atgtggatac ttgtgcagga aatcttcgac agggtgcagc tttacttctc ccaatatctt
     4921 atgaatacgc tcacgccgtt cgtccttact tgaaactccg ctgatgatga gtggctcttc
     4981 caagatctgc tcaacattca taaatggagg caaagcgccg taggggtctt gctgcacata
     5041 tccaatttca aagcgatagc tgcgcaattc agcttctgtc aatgcggtga gacgtttccc
     5101 ttcaaaaaga agctcaccgc ccgttggcac atttagcccc agaatggtct tcatcagcga
     5161 tgacttaccg catccactct ccccgactac tgctacggtt tcccctttac ccaactcaaa
     5221 gctgacgtta tttacggctt tgacatgccc aaccacgcta aagccaaagc ggcgaagctc
     5281 ataccaaaca tgtaaattgt gaatcgcaag caacgtctct gtcggttcat ctattttcac
     5341 agacatgcgt tttgcctctt gtaattctga gatcatagtt gtttatcgaa tttcatcgct
     5401 tctcagtagc agaaccaaac cccgtctgca aacccttcaa acactccatc attccaagat
     5461 gacgacaatc gtgagaaagg aaaacaagca agttcaagcg aatatttttc aattttcgtt
     5521 gaaggtcttc ttctacgcct tcaaaggttg gataacgttc gtctaacaag gcgataaatg
     5581 tctctaatgc ttcataggac ttctgcagat aatctaccaa ttcgtcttta ggcggtatgg
     5641 gcaaagtcgc tgcaatttca tcacttaacc cggttccaac tgtagcattg aggtcggtag
     5701 gaaaacgcca gcgttctgcc aatttctcaa cagcccaaat ctctttgggc tcgccaaagt
     5761 cactgccgag ttgcggggta cgttctaaaa tgatggcttt caaaaaatca gattcgcggg
     5821 ctaagtgcca aagatgaaac ccaatagatg tgctatatcc tcgcggacgc cagcgtagtt
     5881 gttcttcgtt caattcagat gcaaactcaa gcacgacctt atgaatatct ttgaaagaat
     5941 aaagcacgtc atacaatgca agggacggca tggatgaaac tccttggttt attggtgcag
     6001 ccagcacttt actttgcgat ttcctttttg gaaaaccggc ggctcttgat cgcatttctc
     6061 aaatcggaac ggacagcgcg ccgcaaagcg acaacccgtt ggcgggttca tcaaactagg
     6121 tggctgccct gtaataaaca tcgggtctga ttcgctgcgt aagcgtggaa cgctcgccat
     6181 taacatttgc gaataaggat gcagtggtgc agggaagaaa tctttggcat cgcccgtttc
     6241 cacgatctga ccggcataca taattgctac ttcatctgct agctcgctgg aggtggcaat
     6301 atcgtgtgtg atgagaatgt acgaggtacc caattcatgc ttaatacgtt tgagcacatt
     6361 cataatattg gcttgggtca gcaaatccaa agcagaagtg ggttcgtcca aaatgatcag
     6421 atttggcgaa gtaacgagtg ccattgccaa agcaacccgt tgccgcatac cgccactcaa
     6481 ctcgaatgga tagcgactaa tgaagtcagc agggatcccc acatgctgaa acattttgta
     6541 cgccatgcca agcgcctcat ctttattcaa accaagatgc aacatagctg gctctgaaac
     6601 ctgttctccc actgtaatga ccgggttcaa cgcattcata gccgcttgag gtacaagaga
     6661 catactcacc cagcgcacat tctgccggta ttcttcatcg gtaaacggca tcacgtcttt
     6721 tccattaatg accagttcgc cgctatattt cgccacattt ctaggcagca agcgcaacat
     6781 ggcttttgaa agcgatgttt taccgcaccc cgattcaccc agaatgacta cagctcggtg
     6841 aaaccccaag tcgaggtcta cgccgtcaac ggcttgcaca actccttttt gggtacggaa
     6901 gtgcagcttc aggtctttta cttcaagcaa gggtgtcaac tctgtcattg atacgtctcg
     6961 gttacacgtc gcgcaggcgc gggttgaaga tgcgatctaa tacaaagccg aggaaagcaa
     7021 aagccaaccc agtgataatc aacaaaacag caggttccaa cacccagtag taatatccct
     7081 tgtataaagc tccattcgcc tgagcatctt gaataatctt gccccaagtc ggtaggacgg
     7141 ggtctcctaa accgatgacg gcaagagacg cctcaaggaa cacaaaggct ggcacggctt
     7201 gaaccaaacc cggaatcaac aaggggatca tgcgtggaat gaggtaatga aaaataatac
     7261 gccaatttgt tgcgccgtat gcttttgccg cttcaatgta cattgactct ttgacctgaa
     7321 gaaatatggc acgataccca agaatggcac ccgtaaagat attcaagaca atcgttgcac
     7381 tgataatggt ccaaatacta cgggaataaa atgtaccgat catgatcatg atcgcaaaaa
     7441 acggcaacac caaattgact tcggtaattc gttgcaccaa ctggtcgacc cagccgccat
     7501 accatgtgcc aacagcagca atgaccattg ttaagaccga agtgccgata gctgcaataa
     7561 gaccaaatgc caaagcgaca ggggctcccc acaaaagcgg tagggtcaaa tcacggcggg
     7621 catgatccgt ccctgccaag ccatagacct gtccgtgaac cacgagttct acatctatgt
     7681 tcgagttagg ctcaaaagtg gtgccagtaa taacgagctt atagggtccc tttaatacct
     7741 caccggtatc tgggtcagag aacaaggctg ggatggggtc ttctgcccgg agtttggctg
     7801 ccagtttatc gtcttgtgag aagcgatagg tagacttggc tgcggtagca aaatttgaaa
     7861 cacgaatcgt tcgcccatca ggtgtaatcc actccatcga aatgaacggc tgtttctggt
     7921 caaaaagggc attgacataa acgatcatat cttgagggta ga
//
LOCUS       DAOREF010000008         7827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4601_length_7827_cov_3.809701, whole genome shotgun sequence.
ACCESSION   DAOREF010000008 DAOREF010000000
VERSION     DAOREF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_7827_cov_3.809701"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..762
                     /gene="nth"
                     /locus_tag="PKI33_00330"
     CDS             97..762
                     /gene="nth"
                     /locus_tag="PKI33_00330"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNM35469.1"
                     /translation="MTDLKQRALQIHETLLKVFGEPIWRNPLPAIDELVSTILSQNTN
                     DVNRDRAFEALRAKFPTWEAVRDAKEKDVVEAIKPAGLANQKGPRIQQVLKAITEERG
                     ELNLQFLADMPIEDARNWLTKFNGVGPKTAAIVLCFSLNRPAFPVDTHVYRVTGRIGL
                     RPEKMTVEQAHSHLESIFPPETYYAAHLNIIRLGREVCNARKPMCPQCPVKHLCDFKE
                     KTQ"
     gene            816..1283
                     /locus_tag="PKI33_00335"
     CDS             816..1283
                     /locus_tag="PKI33_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNM35470.1"
                     /translation="METPPVSLALEKLNIPHQIFRHETPVDSFEKAASDRGQRPSQIV
                     RTILFRVSEEEFALVLVAGPGQISWKLLRKLLGKSRITMATEEEVISVTGYRPGTVGP
                     FGLLKPARIMVEAGLLKEEEVSIGSGMRDTAVILKTNDLMKALGNVEVVELFE"
     gene            1319..2158
                     /locus_tag="PKI33_00340"
     CDS             1319..2158
                     /locus_tag="PKI33_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35471.1"
                     /translation="MSYKQRTIDFLEIEWATYIERFKRLPELDGVQRVRNQGYERFRD
                     MLAHVLAWWEDVMPVIVAIAENREFEKKKYDFDAFNAEAVAKYKDWDEAEFLAHFEKT
                     RQKAVADLKSMNEAAFEDRRIQGRINGIFIHHAREHLVALSKFLTLDTLEHEWGTYIA
                     RFDASEKKEEFLKKQSAARFGDLLAHNFAWWQEGVNAVQGALKDSSFVYKGPADTDAY
                     NAGIVAKYAGTSEEELRAIFEQKRLEMIELVRGLPDSAFQNPTIEEWLAADIVEHYDE
                     HAL"
     gene            2167..2652
                     /gene="folK"
                     /locus_tag="PKI33_00345"
     CDS             2167..2652
                     /gene="folK"
                     /locus_tag="PKI33_00345"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNM35472.1"
                     /translation="MEHIVYLALGSNMGNRLANLKNAISNLTPQMDVKKKSPVYETPP
                     WGYTDQPAFLNQVVMVETYMEPENLLGHLKRLETVLGREPTFENGPRVIDVDILFYDD
                     LVLNSPPLVIPHPRLHHRGFVLVPLNDIAPELIHPILGKSISELLLDVSRLNINEYKG
                     K"
     gene            2655..3413
                     /locus_tag="PKI33_00350"
     CDS             2655..3413
                     /locus_tag="PKI33_00350"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNM35473.1"
                     /translation="MRIIRYQTPEGTASYGWILEEKVGEVQGNMFGEYRRREARTPFK
                     ELKLLAPCEPSKIVCVGRNYVEHAKELGNEVPKIPLLFFKPPSSIIATSEHIVLPTQS
                     KQVEHEGELVVVMGKRAKNVTAEQAKEYIFGYTIGNDITARDLQKTDGQWTRAKGFDT
                     FCSFGPWIDTDFDPSDAVVTCRVNGQMRQMASTRDMVFNVGTLVAYISSVMTLEPGDL
                     IFTGTPAGVGELKNGDVVDVEIEGLGKLSNPVKA"
     gene            complement(3441..4016)
                     /locus_tag="PKI33_00355"
     CDS             complement(3441..4016)
                     /locus_tag="PKI33_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35474.1"
                     /translation="MHKYLKFFLLENLDTVRRIRVGSFWGVTLLITPWTWLSPFVFFG
                     LHFLLNLSNPRLPLDERLLQSIYFVIAVELTTPLHALGHIISGKLVHSAMDELLITAT
                     RDVNIYHGDQSQISGKVHLVRSLGGPILNILVGLFCILIAPSIPAGFWSGVNTSMIST
                     NLFFGLGGLLPVPSVDGEVIWREIINWMKKK"
     gene            complement(4018..4839)
                     /locus_tag="PKI33_00360"
     CDS             complement(4018..4839)
                     /locus_tag="PKI33_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35475.1"
                     /translation="MNTKSLAISFPLRAWLAVEVLFGVAAVLAVGLAPADTATNFAWP
                     IQPVVMAAVLGAFYITSAPLFLLPFLAKRWEMIRAMILPAALFSTVQLVATFLHWDKF
                     SVGTFPFYVWFASYLLPPPIFIAVYLWHQKQFGVESPIPAEDQIPAWLRTMLLVLGIA
                     LSLIAFIAFFFPSVIIPIFPWKLTPLTARSLSGWLVAVGTLMLSIRQGNSRVRSRLAT
                     PMLILILPSLLMQMGRYANEVNWASPTLWIGLLLFALTGFCGLTLAIGNWRESLT"
     gene            complement(4848..5237)
                     /locus_tag="PKI33_00365"
     CDS             complement(4848..5237)
                     /locus_tag="PKI33_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HNM35476.1"
                     /translation="MKSPTRLYTAVAGIFLLLQGVSTLTFRLVPALDAAFPQLLAVTQ
                     MVPTHSILHILTGILALIVLFHGGEKGSLWFSLGFGAFYTSLALYGFITHMPTMFHLQ
                     LFDHPFHLFLGVLGLAAGGFYFYSNRK"
     gene            complement(5234..6028)
                     /locus_tag="PKI33_00370"
     CDS             complement(5234..6028)
                     /locus_tag="PKI33_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35477.1"
                     /translation="MALVTRNMKVYFAAVGLLALWVGAWGYFVPARVDEAIPWLVPPL
                     HARFLGGMYLSGTAFMIAGIFSRRFDEVKTMVPMIAIWTGMLFIVSLFYLGEFDYSRP
                     QVWIWFGAYLAYPLIALWITWSHRTLNEPHDGTPLPAWAKNYLLVQGVLVTLLSLSLL
                     FAPDLMVSVWPWKITRLLAQIYSAPFLSYGLGSLMLSRRQTWKEARVVLIGTVVFAIF
                     VLLASIIHRELFSLSNASTWIWFGGFAIASLVLGGMTVRSLREKPE"
     gene            6206..7600
                     /locus_tag="PKI33_00375"
     CDS             6206..7600
                     /locus_tag="PKI33_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNM35478.1"
                     /translation="MTTEFETFLQKYPTYKHTEKIDDLRKSDYSRLDAGEHVYFDYTG
                     GGIYAESQVKKHYQLLQENVFGNPHSSNPTSIAATHLVEGTREYILKFFNADPDEYLA
                     IFTPNASGALKLVGESYPFPNGRYLLTFDNHNSVNGIREFAHARGAEVTYIPVMLPEM
                     RVDASQLDLELSRPSKAGHNLFAFPAQSNFSSVKHPLEWIEKAHAHGWDVLLDAAAYV
                     PTSKLDLSKVKPDFVPISFYKIFGYPTGLGALIARKSALAKLHRPWFAGGTITVASVQ
                     GDKYYLAEGAPAFEDGTLDYLNIPALEIGLKHIESIGYEVIGERVKTLTGWLLENLTA
                     MKHSTGEPLVRLFGPNSTEGRGGAVTVNFYDKNGKAFDHRFIESEANKVNISLRTGCF
                     CNPGAGEVALGISRVELDVCFTRPEHKDVMSIDEFRKCIDGKSSGAVRISVGPFTNFN
                     DVQALLSFARGLLS"
BASE COUNT         2228 a         1975 c         1909 g         1715 t
ORIGIN      
        1 gacaagcatc gtgaagtcat tgagaatacg ctacaattga ttaataattt caaccacgaa
       61 cagaaataaa acaactgctt tcttttaata aacctcatga cagacctcaa acaacgtgct
      121 ctacaaatcc atgaaactct gctaaaggtc tttggcgagc ccatttggcg caatcctctg
      181 ccggctattg acgaactcgt ctccaccatc ctttcgcaga ataccaatga tgtgaaccgc
      241 gaccgtgctt ttgaggcttt gcgtgccaag ttccccacat gggaagccgt gcgcgatgcc
      301 aaagaaaaag atgtggttga agccatcaaa cctgccgggc tggcgaatca aaaaggtccg
      361 cgcatacagc aggtgttgaa agccattacc gaagaacgtg gtgaactcaa cctgcaattt
      421 ctcgcggata tgcccattga agatgcccgc aactggctga ccaaattcaa cggcgtgggt
      481 cccaaaactg ctgccatcgt tttatgcttt tctctcaatc gccccgcctt cccggtggat
      541 acccatgtct atcgcgtgac aggtcgcatt ggactgcgcc ccgaaaagat gaccgtggag
      601 caagcacact cccatcttga atccatcttc ccgccagaga cctactacgc cgctcacctg
      661 aacatcatcc gtttgggacg ggaggtctgc aatgcgcgca agccgatgtg cccgcaatgc
      721 ccggtcaaac atctatgtga tttcaaagag aaaactcaat aggactacac tgcgctgtta
      781 tggcgcagtt tttattttca gttagaatgc atgccatgga aacacctccc gtcagccttg
      841 ctttagaaaa actgaatatc cctcatcaaa tcttcaggca tgaaacacct gtggattcgt
      901 tcgagaaagc cgccagtgac cgtggtcagc gcccttcgca aattgtgcga acgattctct
      961 tccgtgtgag tgaagaggaa tttgcgctcg tcctcgtagc aggaccgggt caaatttcgt
     1021 ggaagttgct gcgaaaatta ctcggcaaat cacgcattac catggcaacc gaagaagagg
     1081 tgatatccgt caccggctat cgcccaggga cagtgggtcc gtttgggttg ttgaagcccg
     1141 cccgcatcat ggtggaagcg ggtctcttga aggaagagga agtatccatt gggtcgggca
     1201 tgcgcgacac agccgtcatc ctcaaaacga atgacttaat gaaagcgtta ggaaatgtgg
     1261 aagtggtaga actgtttgag taaaatctaa ctagcatctt atgatctgga gagtatcaat
     1321 gagctataag caacgcacga tcgacttttt agaaattgaa tgggcaacgt atattgaacg
     1381 ctttaaacgt cttcctgaat tggatggcgt acagcgtgtt cgtaaccaag gctatgagcg
     1441 tttccgcgat atgttggcgc acgtcttggc ttggtgggaa gatgtgatgc cagtgatcgt
     1501 ggcaattgca gagaaccgtg agtttgaaaa gaagaaatat gatttcgatg ccttcaatgc
     1561 cgaagccgtg gcaaagtata aagattggga tgaagccgag tttttggctc actttgagaa
     1621 aacgcgtcag aaagccgtag cagacctaaa gtctatgaat gaggcggcgt ttgaagatcg
     1681 tcgcattcag gggcgcatca acggcatctt catccaccat gcccgcgaac atctggtggc
     1741 attgagcaaa ttcctcacgc tcgatacgct tgaacatgaa tggggaactt atattgcgag
     1801 attcgatgct tcagagaaga aagaagaatt tttgaagaaa caaagtgcag ctcgttttgg
     1861 cgacctgttg gcacataatt ttgcatggtg gcaagaaggc gtgaatgctg tgcaaggtgc
     1921 tttgaaagat tcaagctttg tgtataaagg tccggcagat actgatgcct acaatgcagg
     1981 cattgtggca aagtacgcag gaacgagtga agaagaatta cgagccatct ttgagcaaaa
     2041 acgtttggaa atgatcgaac tcgtgcgcgg acttcctgat agcgccttcc aaaacccgac
     2101 cattgaagaa tggctcgccg ccgatattgt ggaacactac gacgaacacg ctctctaagg
     2161 agccacatgg aacatatcgt atatcttgca cttggaagta atatggggaa tcgactcgcg
     2221 aatttgaaga acgcgatctc aaacctcacg cctcaaatgg atgtgaaaaa gaagtcgccg
     2281 gtatatgaga ctccgccttg gggatataca gaccaacctg cattcttgaa tcaggtggtg
     2341 atggtggaaa catacatgga gcctgaaaat ttacttggac atttaaagcg cctcgaaacc
     2401 gtcctcgggc gggaacctac cttcgagaat ggtccacgcg tgatcgacgt tgacatctta
     2461 ttctatgacg acctcgtgct caattctcct ccactggtca ttcctcaccc acgtttgcat
     2521 caccgcggat ttgtgctggt gccactaaac gatattgccc ctgagctgat ccacccgatt
     2581 ttaggaaaat ccatcagcga attgttattg gatgtgagcc gattgaacat caacgaatat
     2641 aaagggaagt agacatgcgc atcatccgtt atcaaacccc ggaagggact gcctcatacg
     2701 gttggatcct tgaagaaaaa gttggcgaag tgcaagggaa tatgttcggc gaatatcgtc
     2761 gccgtgaagc gcggactccc ttcaaggagt tgaaacttct cgcgccatgt gaacccagca
     2821 agatcgtgtg cgtggggcgt aactatgtag aacatgccaa ggaattgggc aatgaagtac
     2881 cgaagattcc gctactcttt ttcaaaccac cttcctcgat cattgcaacc agtgaacaca
     2941 ttgttcttcc aactcaatcc aagcaagtag aacatgaagg cgagttggtg gtggtgatgg
     3001 gcaaacgtgc caagaatgtc acagccgagc aggcgaagga atacattttc ggctatacca
     3061 ttggcaacga tatcacagct cgtgacctgc aaaaaacgga cggtcagtgg acgcgcgcca
     3121 aaggcttcga caccttttgt tccttcggtc cgtggatcga tacggacttt gacccgtcgg
     3181 atgcggtcgt cacctgtcgc gtcaacgggc agatgcggca aatggcatcc acccgagata
     3241 tggtgttcaa cgtgggtacc ttggttgcct atatttcctc agtcatgacc ctcgaacccg
     3301 gtgacttgat ctttaccgga acacccgctg gggttggcga gttgaaaaat ggtgatgtgg
     3361 tcgatgtaga aatcgaagga ctgggaaagt taagtaatcc ggtaaaagca taaaaaaagc
     3421 cccgcaaatg cggggctttt ttatttcttc ttcatccaat tgataatctc tcgccaaatc
     3481 acctccccat ctaccgaagg gacaggcaac agaccgccca acccgaagaa tagattggtg
     3541 gagatcatgc tggtgttcac gccgctccaa aacccagcgg gaatggatgg agcgatgagt
     3601 atacaaaaca aacccaccag aatattgaga atgggtccgc ccaacgaacg gacgagatga
     3661 accttaccgg aaatttggct ttggtcgccg tgatagatgt tgacatcacg cgtggcagtg
     3721 atgagcaatt cgtccatggc gctgtggacg agtttgccac tgatgatatg accaagggca
     3781 tgaagcggag tagtcaactc gacggcgatg acaaagtaaa tggactgaag cagcctttcg
     3841 tcgagtggga gccgtgggtt cgaaaggttg agcaagaaat gcaacccgaa aaagacgaaa
     3901 ggactgagcc atgtccacgg ggtgatcaga agagtcacac cccagaaaga accaactcga
     3961 atccggcgaa cggtatcaag attttcgagc aaaaagaatt tgagatattt gtgcatgtta
     4021 ggtcagagac tcgcgccaat ttccgatggc aagggtcaat ccacagaacc ctgtcaatgc
     4081 aaaaagcaac aaaccgatcc acaaggttgg gcttgcccaa ttgacttcgt tggcatagcg
     4141 ccccatttgc atgagcaaag atggcaggat gagaatcaac atgggcgtgg caaggcgcga
     4201 gcggacgcgg ctatttcctt ggcgaataga aagcatcagc gtgcctactg ccacaagcca
     4261 accgctcagt gaacgagccg tcagcggagt taacttccac gggaagatgg ggatgatgac
     4321 actcgggaag aagaaggcga tgaatgcaat cagagaaaga gcaatgccca gcactaacaa
     4381 cattgttctt agccaagcag ggatttggtc ttcggcaggg atgggcgact caacaccaaa
     4441 ttgcttttga tgccaaagat atactgcgat gaaaattgga ggtggaagta gatagctggc
     4501 aaaccaaaca tagaacggaa aagtaccgac ggaaaattta tcccaatgca aaaaggtggc
     4561 gacaagttgc accgtgctga acaacgccgc cgggaggatc atggcacgga tcatctccca
     4621 acgtttggcg agaaaaggca acaagaataa aggagccgat gtaatataga acgcgcccaa
     4681 aactgctgcc atcactacag gctgaatggg ccaggcaaag ttcgtagccg tatcggcagg
     4741 ggcaagacct accgccaaca ctgccgcgac cccgaacaac acttcaactg ccaaccaggc
     4801 tcgtaatgga aatgagattg ctaaactctt tgtgttcata tgaatcccta ttttcgattg
     4861 gaataaaaat aaaacccgcc agccgccaag cccagtacgc ccaagaacaa gtggaaggga
     4921 tggtcaaaaa gctgtaagtg gaacatggtc ggcatatggg tgataaaccc atacaatgcc
     4981 aaactggtat aaaacgctcc aaagccgagc gaaaaccaca ggcttccttt ttcaccgccg
     5041 tggaacaaga cgatcaatgc cagaatcccg gtgaggatgt gaagaatgga atgggtcgga
     5101 accatctgcg tcacagccag taattgcgga aaagccgcat ccaacgcagg gacaagccga
     5161 aatgtcaacg tggatacgcc ttgcaataac agaaaaatcc ctgccacggc tgtatataaa
     5221 cgagttgggc ttttcattca ggtttctccc gcaacgaacg gacggtcatg ccgcccagca
     5281 cgagagatgc aattgcaaag ccaccaaacc aaatccatgt agaggcgttg gagagcgaga
     5341 aaagttcacg atggatgatc gacgccaaca gcacaaagat ggcgaagact accgttccga
     5401 tcaaaaccac gcgcgcttct ttccatgttt ggcgacgact aagcatcaaa cttcccaagc
     5461 cgtacgacaa gaaaggtgcg gaatagattt gtgccaacaa acgggtgatt ttccacggcc
     5521 aaacgctcac catcagatcg ggcgcgaata gaagcgacaa agacaataag gtaaccaaca
     5581 caccttgcac aagcagataa ttcttcgccc atgccgggag tggcgtgcca tcatgcggtt
     5641 cgttcaaagt gcggtgagac catgtaatcc acaaagcgat caatggatac gccaaataag
     5701 ctccgaacca gatccatacc tgcgggcggc tgtaatcgaa ctcgcccaaa taaaaaagcg
     5761 agacgatgaa caacatgccc gtccaaatgg cgatcatcgg caccatggtt ttcacttcat
     5821 caaaacggcg ggaaaagatg ccagcgatca tgaacgccgt acccgaaaga tacatgcctc
     5881 ccaaaaagcg cgcgtgcaag ggcggcacca accacggaat agcttcatcc acccgggctg
     5941 ggacgaaata tccccacgcc cccacccaca atgccagcaa ccccactgcg gcaaaataca
     6001 ctttcatgtt acgagttacc aatgccataa acacctcact aaaagactca agaacgatga
     6061 aactgcgtat ttataaactt tgcttggctg attatacata taaccttggt taggttaaca
     6121 gcctgtcttt ggttataaag aaacccaatg aagaactccc acccaacttc caagtgtggt
     6181 taaatagatt tcaggagccc cacgaatgac tacagaattc gaaaccttcc ttcagaaata
     6241 ccccacctac aaacacaccg aaaaaataga tgaccttcgc aaaagcgatt attcccgcct
     6301 cgatgcgggc gagcatgttt attttgatta caccggcggc ggtatctatg ccgagtcaca
     6361 ggtaaagaag cattatcagc ttctacaaga aaatgtcttc ggcaacccgc attcctccaa
     6421 ccccacttcc atcgccgcga cacatctggt cgaagggaca cgcgagtaca tcctcaagtt
     6481 cttcaacgcc gatccagatg aatatctcgc catcttcaca cccaatgcca gcggtgcgct
     6541 caaactcgtc ggcgagagct atcccttccc gaacggacgt tacctcctga ccttcgacaa
     6601 ccataattct gtgaatggga ttcgcgaatt cgcacatgcc cgcggcgcag aggtgaccta
     6661 cattccggtc atgctgcccg aaatgcgggt ggacgcctct caactcgacc tcgaactgtc
     6721 tcgcccttcc aaggctggtc ataacctgtt tgctttcccc gcccaatcga acttctcctc
     6781 cgtcaagcat ccgctcgaat ggatcgagaa agcccacgcc cacggctggg acgtgctcct
     6841 agacgcggct gcctacgtcc ccaccagcaa actcgaccta agcaaggtca agcctgattt
     6901 cgtaccgatc tctttctata aaatattcgg ctacccaacc gggcttggcg cactcatcgc
     6961 aagaaaatca gccttggcga aacttcatcg cccctggttc gccggtggga cgatcactgt
     7021 cgcatcggta caaggcgaca aatactatct cgccgaaggc gcacccgcct ttgaagatgg
     7081 cacgctcgat tacttgaata ttcccgccct tgaaattggg ctcaagcata ttgaatccat
     7141 cggctatgaa gtgatcggcg aacgagtcaa aacgctcact ggctggctgt tggaaaatct
     7201 caccgccatg aagcacagca ccggcgagcc tctcgtgcgc ctgttcggac ccaactccac
     7261 cgaaggcaga ggcggcgcag tgaccgtcaa cttctatgat aagaacggca aagctttcga
     7321 tcatcgcttc atcgaaagtg aagccaacaa agtcaatatc tccctccgca ccggctgctt
     7381 ctgcaacccc ggcgcaggcg aagttgctct tggcatctcc cgcgtggagt tagacgtctg
     7441 cttcacccgc cccgagcata aggatgttat gtccattgac gagtttcgca agtgcattga
     7501 cggcaaatcc tccggcgcgg tgcgcatctc cgtcggtccg ttcaccaatt tcaatgatgt
     7561 gcaagcgttg ttgtcgtttg caagagggtt gttgagttga attggctaaa gcaagaatta
     7621 attatcgatg gtgttccgat aaaactacaa ttgtctttta tgaatttcga aacgcttctt
     7681 atagaagtta cttgcatgga agattcatgg cgcaattata tttcctctgg gattagattt
     7741 cttgaaataa aaggaatcac tccaaacggg aaagaaataa atggtaaatt tcaaataatt
     7801 gataacatcg atgatgtttt tgttcta
//
LOCUS       DAOREF010000009         7806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4626_length_7806_cov_3.952651, whole genome shotgun sequence.
ACCESSION   DAOREF010000009 DAOREF010000000
VERSION     DAOREF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4626_length_7806_cov_3.952651"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..569
                     /locus_tag="PKI33_00380"
     CDS             147..569
                     /locus_tag="PKI33_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNM35479.1"
                     /translation="MKDLFIKLFMFINAQIIRLSRGKVGSKLGTQTILILHTIGRKSG
                     EERAIPIAYFKYENKYLIVASNWGKDQQASWYLNLKKNPSATLEVQGKVISTNAREAE
                     GEEYAHLWSFVTKLHPPYSDYQKLTARRIPIMIFDPIG"
     gene            600..1352
                     /locus_tag="PKI33_00385"
     CDS             600..1352
                     /locus_tag="PKI33_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35480.1"
                     /translation="MFIKKYTLLVLPFLFILSGCGLNRASVPTPLPTYTQPSSPTSPP
                     ATETALPTSTAEFVATATNIATATPPPVCHVGETIESANNGSLPSYIDIVKVSTKLSG
                     SKFSATFTMNWLPDQIMIDRNILGYGAAEIAWGVAIDVDNDPNTGSSIPLTSSGYGYD
                     YVLQAANFKEGEEVQGGLQALFSDKVNVWEVFADGSNSVLYQGKINVDTATASITLSG
                     DIKGIKKDSYLQFFAIYFPTASQQLTDEICQR"
     gene            1349..1699
                     /locus_tag="PKI33_00390"
     CDS             1349..1699
                     /locus_tag="PKI33_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNM35481.1"
                     /translation="MNREPVSISNAEHYIWGQVCDGWHLLRQDALSVIQERVPAGRTE
                     VMHYHTSARQFFYVLEGEGQIAFEDQTVTLKKGEGLEIQPQVWHRFENNSQTDVHFLV
                     ISSPKAQGDRVNVE"
     gene            1721..2194
                     /locus_tag="PKI33_00395"
     CDS             1721..2194
                     /locus_tag="PKI33_00395"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNM35482.1"
                     /translation="MSNVKEELLQMYKKSQADITSQLDEIDKNRKIYPLWTVREIIAH
                     LSGWDDSITGMLNALLKGDVPPTPAARGVDVYNAETVSTREGLSYDHVIREYYKTREK
                     LFALISQANDDILSKPSILPWGEEGSVAMLVKFFSEHEAEHAEDIKKFIETATQK"
     gene            2256..5441
                     /locus_tag="PKI33_00400"
     CDS             2256..5441
                     /locus_tag="PKI33_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNM35483.1"
                     /translation="MKSRILALLNIRRGEEKNVFLMLTQYFFMGAAMLFVQSASLALF
                     FTAWDATAMPYIYLGIAVIVSTITAIFLKISERTSLARFLILSVLFVLFGSIALRIGL
                     AFTSSKWLLLALPIWSQTLVNITVTAFWTLAGNIFDVRQGKRIFGLMNAGSWLSYVVL
                     GPFTTPLVNLIGTENLYLVIAACLLISFFLQQAVIHANPSTETNQETTDQQSQQTSIA
                     QLFRIRYIALIFALITIWRIAYFILDNIFYDRAALQYPTADSMAGFIGGFFAITGLLG
                     FITDMFLTGRIISRFGLRAGLLATPTLTLLFTAALAATGTLKPEMLPAIFWLAVAGKF
                     TNEGLGFSLDQTADNLLYQPIHERIRARAQTVTEGIIQPLAIGLAGGMLLLFNTALKF
                     NAIQLAYIYIAFAIIWIIICIALIRAYPVALTEALHKRRFGDKGILLTDASSIQIVRM
                     ALKSQHASEVLYALDLLEKGESDTFIDDLETQIRSPMPEVRKDVIQRIERLKITKLLR
                     DIQIQLRTETDPNVREIASRALIVLGADSSTALSLLAQEDAATQRGAMIGALRILRES
                     KDSNAYERLLELTKSKQVQDRVYAAQLMAEAANPILSNVLLALLSDEDLQIKKAALRA
                     AAKFQHPDLYSKIIPALGSPLTRSLAFNALVEGGNHAIPSIIEALKDISLHRRIHLRL
                     IRACSHIKSEAAIHVLEGLIHHSNVSVRSRALTALHDCRYKPEGESAFGVSEQVTAES
                     RRAAWLLGVMHDLGKSTKTEVLVRAASHDLDGTIDRLFHLFEFIYEPQAIQRAQHAIQ
                     RKDANRKSYAIEVLDTTLSKPHKTAFIPLLEDFAPKETLEKMGSEYKQTFLPPAGRVM
                     DILTNPLAKQNPWLVATAIEFADSMGISLDSEVTDSLSSSNESLLARMVLQKRKEKMM
                     LSTVERVIVLKSLSMFEKTPDEALAELAELVQEMVVQPGDEIIREGETGDSLYIIVDG
                     KVEVVDDNRILNQLGARAVFGELSMLDSSPRTATIRAIEETSLLRLDQTSFYEIMSDY
                     VEVAMGTIQLLTRNLRARTGDVMELSRMLGQ"
     gene            5455..5847
                     /locus_tag="PKI33_00405"
     CDS             5455..5847
                     /locus_tag="PKI33_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35484.1"
                     /translation="METKPALDSKLVEEFVGNSHGNFARVKELLEQEPALVNATWDWG
                     GGDFETALGAAAHMGNKQIANYLLEHGARLDIFAAAMLDQLDVVKAALEAYPEAVNTP
                     GPHGIPLYVHAEAGDAKSVLEYLNSLEG"
     gene            5913..6665
                     /locus_tag="PKI33_00410"
     CDS             5913..6665
                     /locus_tag="PKI33_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35485.1"
                     /translation="MKKRNQVVIGLIAAIIMLACACPVSGLPFGSEEQPTPGLPILPV
                     ATIVDAPATEAPVQPAANVLLSDDFSSPSAEMETYSGDGGSAGTENGVYVVRSTADLW
                     QWGKSNSQFDNAIFEADITMIAGPTNNNAGFGIICRLSERDDTSIDGYMFAISGDGYY
                     TIRHIVSSNMTALVDWTESSVINQGNASNHLRATCNGSDLRLEVNGQEVASAIAPADG
                     PTNGTMAFAAISFETDQPYAEGHFDNLTVSQP"
     gene            complement(6713..7681)
                     /locus_tag="PKI33_00415"
     CDS             complement(6713..7681)
                     /locus_tag="PKI33_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35486.1"
                     /translation="MRVCVLSDEYIEDFNPAPYLKDYDWKMVTLTAPVEEKIKALAES
                     GEFDVFLNVCEGYEFEDEEDEELGYEASEVVEALEALNLPFTGADSRCFDPTREEMQT
                     IADANGVGFAKGYRVHSVEEAEQLVKNLRYPIMVKHPKSYGSTGMFKDSRADTLEQVL
                     TQVKRVCSEFGAARMEEFIVGKEFNVLVVDNPADMDNPFVYPPAELIFPDGEEFWHTD
                     IKWDYNIPFDFKEVTEPELKAKLQNEGKRMFQAMGISGYGRCDIRMNEKGELFILEIN
                     PNPAIMLPPKEYGPADYMILYDNDGYKGFFDRIFEAAFLRQKMRAK"
BASE COUNT         2179 a         1859 c         1691 g         2077 t
ORIGIN      
        1 aaagcatcag cagtgcggat atgcgcatgt atgaaatgaa aactgagcat aagcaaagcg
       61 cttcataaaa atgttatttg tctctacagt aagatttttt ccattctccc tatctctttc
      121 atatcatcac acccaacgag gacgatatga aagatctttt catcaagctt tttatgttca
      181 tcaacgctca aataatccgc ctgagccgtg gaaaagtggg cagcaaatta ggaacacaaa
      241 ctatccttat tttgcatacc attgggagaa aatctggcga agaacgcgcc atccctattg
      301 cttattttaa gtatgaaaac aaatacctga tcgtagcatc caattggggt aaagaccaac
      361 aagcaagttg gtacctcaac ctgaaaaaga atccatcggc aacccttgaa gtgcagggaa
      421 aagtcatttc cacgaatgca cgtgaagcag aaggtgaaga atatgcgcac ttatggagtt
      481 ttgtgacgaa actccatccg ccttatagtg attatcagaa attgacagcc cgccgtatcc
      541 ccatcatgat atttgaccca atcggataaa attaccgaaa aagattggtt taagttttca
      601 tgttcatcaa aaaatatacg cttcttgtcc tcccttttct gttcattctt tctggctgtg
      661 gtcttaatcg tgcgtcggtt cctactccct tgccaacata tacccaaccc agttcaccaa
      721 caagcccgcc tgcaacagag accgccctac ccacgtcaac cgctgaattc gtagccactg
      781 caacaaatat tgctacggca acaccacctc cagtctgcca tgtcggtgag acgattgaaa
      841 gcgcaaacaa cggctctctg ccaagctata ttgatattgt aaaagtatca accaaattaa
      901 gcggaagcaa attttcagcc acatttacga tgaattggct gccagaccag atcatgatcg
      961 accgtaacat tcttggttat ggggcagcag agattgcttg gggggttgcc atcgacgtag
     1021 ataatgaccc taacaccggc tcctccatcc cgctcaccag ttctgggtat ggctatgact
     1081 atgtccttca agcagctaac ttcaaagagg gcgaagaagt gcaaggcgga ctgcaagcct
     1141 tattcagtga caaagtaaat gtatgggaag tatttgcaga tggcagtaat tctgtattat
     1201 accaaggcaa gatcaacgtg gatacagcca ccgcctccat tacgctaagc ggagatatca
     1261 agggcatcaa aaaagattcc tacctgcaat tctttgctat ctatttcccc actgcatctc
     1321 aacagctaac agacgagatc tgccaacgat gaacagagaa ccggtctcca tatccaatgc
     1381 ggaacattat atttggggtc aggtttgcga tggctggcac ttactcaggc aagatgcatt
     1441 gagtgttatc caagaacggg ttcctgctgg caggactgag gtgatgcatt atcacacgtc
     1501 tgctcggcag ttcttttacg ttctcgaagg agaaggacaa attgcttttg aagaccaaac
     1561 cgtcacactg aaaaaaggtg aagggctgga gattcagcct caagtctggc accgattcga
     1621 aaacaactcc caaacagatg tacatttttt agtcatctca tcacccaagg cgcaaggcga
     1681 ccgggtgaat gtcgaataaa ttatctatca aggaaatgaa atgtcgaacg tcaaagaaga
     1741 acttctgcag atgtataaaa aatcgcaagc tgatatcacg tctcagcttg atgaaatcga
     1801 taagaaccga aaaatttatc ccttatggac agttcgcgaa atcatagcgc atctctccgg
     1861 ctgggacgac tctattacag gaatgttgaa tgcgctccta aaaggcgatg tcccaccaac
     1921 ccctgctgcc cgcggtgtag atgtatacaa tgctgaaacc gttagcacac gtgaaggctt
     1981 gagttacgat catgtcatcc gtgagtatta caagacgcgc gaaaaacttt tcgcactcat
     2041 tagtcaagcc aacgacgaca ttctttccaa gccatctata ttgccttggg gtgaagaagg
     2101 aagtgtagca atgcttgtga agttcttttc agagcatgaa gccgaacatg ctgaagatat
     2161 caagaaattc attgaaacag ccacacaaaa ataagccaaa taaccgcaga aatttctgcg
     2221 gttattttct tctcaatacg ataaaatagc ctttcatgaa gtctcgcatc cttgcgttac
     2281 tcaacatccg ccgtggcgaa gaaaagaacg tttttcttat gctcacccaa tattttttca
     2341 tgggtgcagc catgcttttc gtacagtctg cttcgctggc attgttcttc actgcctggg
     2401 atgcaaccgc catgccctat atttatctgg ggatcgcagt cattgtatcc acgatcacag
     2461 cgatcttcct caagatatca gagcgcacct cactggcgcg ctttttgatt ctaagcgttt
     2521 tgtttgtcct atttggaagt attgctcttc gcattgggct ggcatttaca agttcaaaat
     2581 ggttattgct ggcattgccc atttggtcgc agacgttggt caacatcacc gtcacagcct
     2641 tttggactct ggctggcaat atcttcgatg tgcgtcaagg caaacgcatt tttggcttga
     2701 tgaatgcggg ttcgtggctc tcgtatgtcg tcctcggacc tttcactacc ccgctggtaa
     2761 atttaatcgg cacagaaaat ctatatcttg tcattgcggc ttgcttgtta atttcattct
     2821 ttctgcaaca agctgttatt cacgccaacc ccagcacaga gacaaaccaa gaaacaactg
     2881 atcaacaatc gcaacaaact tccattgcac aactttttcg cattcgttat attgctttga
     2941 tctttgccct catcacgatt tggcgcattg catatttcat tctcgacaat attttctacg
     3001 accgcgccgc tttgcaatac ccaactgcag actcaatggc tggctttatt ggcggatttt
     3061 tcgccattac tggcttgctt ggtttcatca ctgatatgtt cctgacgggg cgcatcattt
     3121 ctcgtttcgg cttgcgtgct ggtctcttag ccactcctac gctgacactg ctcttcacag
     3181 cagcactagc tgccactggc acactcaaac ccgaaatgct tcctgctatt ttctggctgg
     3241 cagtcgcagg caaatttacc aacgaaggtt tgggtttttc attagaccaa actgccgata
     3301 acttgctcta ccaacctatc cacgaacgga ttcgcgcccg tgctcaaact gtgaccgaag
     3361 ggattatcca accattggca atcgggttgg caggcggcat gcttttactt ttcaacacag
     3421 cgcttaaatt taatgccatc caacttgcat acatttacat tgccttcgcc ataatttgga
     3481 tcattatttg catagcactt atccgcgcat atcctgtggc acttacagaa gccttgcaca
     3541 aacgacgttt tggcgacaag gggattctcc taacggatgc tagcagtatt cagattgtcc
     3601 gcatggcatt gaaaagccag catgcatctg aagttctgta tgcgcttgac ctgttggaaa
     3661 aaggggaaag tgatactttt atagatgacc ttgaaacaca aatccgctcg cccatgcctg
     3721 aagtgcgaaa agatgtcatc cagcgcatcg aacgtttgaa gattaccaaa ctgctacgag
     3781 atattcagat tcagctacgc acagagactg accccaatgt acgagaaatc gcttcacgcg
     3841 cactcatcgt ccttggcgca gactcaagta ctgctctgag tctcctcgcc caagaagatg
     3901 ctgctaccca acgtggcgct atgattggcg ccttaagaat attgagagag tcaaaagaca
     3961 gcaacgctta tgaacgcctt ttggaattga caaaatctaa gcaggttcaa gatcgggttt
     4021 atgcagcaca gttaatggct gaagctgcaa atcccattct ttcgaacgtg cttttagccc
     4081 ttttaagcga tgaagactta caaataaaaa aagctgcttt acgcgcagca gctaaattcc
     4141 agcacccaga tttatattca aagatcatcc ctgctttagg gtcgccactg acacgcagcc
     4201 ttgctttcaa cgcacttgtc gaaggcggaa atcatgcaat tccatctatt attgaagctt
     4261 tgaaagatat aagtctacac aggcgcatcc acttgcgttt gattcgcgcc tgctctcata
     4321 tcaagagtga agcagcaatt cacgttttgg aagggttaat tcaccactcg aatgtaagcg
     4381 tccgttcgcg agcgttaacc gctttgcacg attgcagata caagcccgag ggagaatccg
     4441 cttttggggt tagtgaacag gtcacggcgg agtccagacg cgctgcttgg ttgttgggag
     4501 tcatgcatga tttgggcaaa tccaccaaaa ctgaagtctt ggtgcgggca gcgagtcatg
     4561 atctggatgg cacgatagac cgcctctttc atctctttga gtttatctat gagccacaag
     4621 cgattcaacg tgcacaacat gccattcagc gaaaagatgc aaatcgtaaa tcctatgcga
     4681 ttgaagtatt agatacaact ctcagcaaac cacataagac agcttttatt ccattgctgg
     4741 aagattttgc gcccaaagaa acgctagaga aaatgggaag tgaatacaag cagaccttcc
     4801 taccgccagc cgggcgcgtt atggatattt tgaccaatcc gctggcaaag caaaacccat
     4861 ggctggttgc aactgcgatt gaatttgcag atagcatggg aatttcgtta gacagcgaag
     4921 tgacagattc attatcttct tctaacgagt ctttactagc gcgtatggtt ctgcaaaaaa
     4981 gaaaggaaaa gatgatgtta tccactgtag aacgagtgat tgttttaaag tcgttgagca
     5041 tgttcgaaaa aacaccagac gaagctctcg ccgagttggc agagctcgtg caggaaatgg
     5101 ttgtacaacc cggagatgag atcatccgtg aaggagaaac tggcgacagt ttgtatatta
     5161 tcgtggacgg caaagtagaa gtggtggatg ataaccgcat cttgaatcaa cttggagcac
     5221 gggctgtctt tggtgagctt tccatgctag actcttcacc acgcactgca accatccgcg
     5281 ctatagaaga gacatccctc ttgcgccttg atcaaacgtc gttttatgaa attatgagcg
     5341 attatgtaga agtagccatg ggcacgattc aattattaac ccgtaactta cgtgcccgca
     5401 cgggtgatgt aatggaacta agccgaatgt taggtcaata gagaaggaga gaaaatggaa
     5461 accaaacctg ctttagactc aaaacttgtt gaagagtttg ttggaaattc acacggaaat
     5521 ttcgcgcgtg tgaaagagtt actcgaacaa gagcctgcgc ttgtcaatgc aacctgggat
     5581 tggggcggcg gagactttga aactgctctt ggtgctgctg ctcacatggg caataagcag
     5641 atcgccaatt acctacttga gcacggtgcg cggttggata tttttgccgc cgccatgctg
     5701 gatcaattag atgtggtcaa agctgcatta gaagcatatc ccgaagcagt caacacgccg
     5761 ggtccgcacg ggattccgct ttacgtccat gcagaagcag gagacgcgaa gtctgttttg
     5821 gagtatctca attcccttga gggatagtac caaagggttc ttggactgaa caaattgggt
     5881 cgatataatt tagaaatact ttaggagaaa aaatgaaaaa gagaaatcaa gtagttatag
     5941 gtctgattgc cgcgatcatc atgctcgcat gtgcttgccc cgtttcaggt ttgccttttg
     6001 gtagtgaaga acaacccaca cctgggcttc ctattttgcc agttgcaacc atagtagatg
     6061 cgcctgccac agaagcccca gtacaacctg ctgcaaatgt tttgctcagt gacgatttta
     6121 gctcccccag cgccgaaatg gaaacttatt caggcgatgg cggcagtgct ggaaccgaaa
     6181 atggtgtgta tgtcgtccgc tctacagctg acctgtggca atggggtaag tccaattcgc
     6241 agtttgataa tgcaatcttt gaagcagata taaccatgat tgcaggtccg accaataaca
     6301 acgctgggtt tggcattatc tgtcgcttga gcgaacgtga tgacacatcc atcgatggct
     6361 atatgtttgc gatcagtgga gatggttatt acactattcg ccatattgtc tcaagcaaca
     6421 tgactgcact ggtagactgg accgaatcaa gtgtgatcaa ccagggcaac gcctcgaatc
     6481 acctgcgcgc cacctgcaac ggcagtgact tgagactcga agtcaatgga caagaagtcg
     6541 cttccgctat cgcgccggca gatggtccaa ccaacggaac catggctttc gccgccatct
     6601 ctttcgaaac cgatcagccc tatgctgaag gtcattttga taatttgaca gtcagccagc
     6661 cgtaataatt agtagaaata aaaaagacgt gcttggcacg tcttttttat ttctactttg
     6721 cacgcatttt ctgccgaaga aatgctgcct caaagatacg gtcaaagaag cctttatacc
     6781 catcgttatc atacaagatc atataatctg caggaccata ttctttaggt ggcagcataa
     6841 tagcaggatt ggggttgatc tccaaaataa acaactcgcc tttttcgttc atacggatgt
     6901 cgcagcgtcc ataaccagaa atacccattg cctgaaacat tcgttttcct tcattttgaa
     6961 gttttgcctt tagttcaggt tcggtcactt ctttaaaatc aaagggaatg ttgtaatccc
     7021 acttaatatc ggtatgccaa aactcttccc catctgggaa gatcaactcg gcaggcgggt
     7081 aaacgaaggg attgtccata tcagccgggt tgtcaaccac caacacgttg aactcttttc
     7141 caacaataaa ttcttccatg cgggcggcac cgaactcgct acaaactcgt tttacctgtg
     7201 tgagaacttg ctcaagagta tcagctcgcg agtctttgaa catgccggta ctaccataac
     7261 tcttgggatg tttcaccata ataggatagc gcaaattttt gacaagttgt tcagcttctt
     7321 ccacgctatg aacacgatag ccctttgcaa aaccgactcc gttcgcatca gcaatcgttt
     7381 gcatttcttc acgggttggg tcaaaacaac gggagtctgc gccggtaaaa ggtaaattga
     7441 gcgcctccag agcttctaca acttcacttg cttcatatcc aagttcttca tcttcctcgt
     7501 cttcaaattc gtatccttca cagacattta aaaagacatc aaactctcca gattcagcca
     7561 acgctttgat cttctcttcc actggggctg tcaacgtgac cattttccag tcgtaatctt
     7621 ttagataagg agcagggttg aaatcttcaa tgtattcatc agacaaaaca cagactcgca
     7681 tagggtctcc aaatttttga aggatacgca aataacagct acacaaacgc cgctgtctaa
     7741 atgcgtttgc gattatccac ccatttccga ttcagcaatt gattttttaa gaaaaataga
     7801 gcagat
//
LOCUS       DAOREF010000010         7722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4690_length_7722_cov_4.748402, whole genome shotgun sequence.
ACCESSION   DAOREF010000010 DAOREF010000000
VERSION     DAOREF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4690_length_7722_cov_4.748402"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKI33_00420"
     CDS             complement(<1..632)
                     /locus_tag="PKI33_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35487.1"
                     /translation="MKILFLFLDGIGLGEDNPDTNPFLRANMPYLESLLGGKKMTKSA
                     APFESDVLSLKAIDPNLGVKGLPQSATGQAVLVTGINIPAELGYHYGPKPNPEVAQYL
                     NGHTIFSKTVNAGKKATLLNAYPPRYFDGIDSGKRLYSSIPLALTNAGISLFTEKDYF
                     TGTALSADFTGEGWREFLGFPDAPLFDPETAGTKLAELASQYDFSFFEYWA"
     gene            complement(676..1251)
                     /locus_tag="PKI33_00425"
     CDS             complement(676..1251)
                     /locus_tag="PKI33_00425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM35488.1"
                     /translation="MNNLFRRLKFNYNYIAKKTPWDSGISPPELYDFMAAHSPGRALD
                     IGCGTGTNLITLANAGWQAEGFDFASRAVTIAKRKIQKAGVTVKVFTDDATQMKQVTP
                     PFDLALDLGCFHSITNKADYLTQLTRVLNPSGFWLVYGFIKPDPALTGPGITTADLDL
                     IQRHNLTLVSRKDGFDRRDRISAWFLWQANS"
     gene            complement(1244..1735)
                     /locus_tag="PKI33_00430"
     CDS             complement(1244..1735)
                     /locus_tag="PKI33_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNM35489.1"
                     /translation="MGDIFGLYSFVFIAVGFAFVAGVILLSNKPKWNDYLAFGVIVGG
                     LLVAWTILHPRQTLLMDDAKQVKDMIGAGTPVLLEFQSPYCIRCVSIKPIVDELEEEI
                     DPADIHIIRLNIQEQIGMELAPLYDFVATPTFIYFDSEGNEIWRMVGDFDPQRVRDSL
                     ANE"
     gene            1857..3938
                     /locus_tag="PKI33_00435"
     CDS             1857..3938
                     /locus_tag="PKI33_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNM35490.1"
                     /translation="MKEYTTEFIRNVALVSHGGAGKTMLAEAFLHVTGATTRLGKVED
                     GTTISDYDDEEHKRKISIYTSVIPVEHKNYKINFLDAPGYTDFVGEMVSALSVADGAI
                     ILVDAVAGIEVGTELAWRYCDQFNLPRFFVINKMDRENADFQKTYDAITEFVEAHGKR
                     VVQVHIPIGEKQNFKGVVDVIGMKSYMGDGKTIAEIPAELKEAADKAHFAMVEDAAEG
                     EDQLMEKYLENGSLSDEEMVRGLEDVVYAGQFVPVFCAAGAHEIGTIALLNDIIDLIP
                     SPAQAVKRMAQGKDGAEELKATDQGPLAAYVWKTTADPFVGKMTYFRVLSGSLQADAH
                     VWNQNKGADERMSGMHFQRGKETIAAKSIHAGDLAVVSKLTVTATGDTFCDKGHPLTV
                     EKPAYPAALYRVAVAPKTQADAAKISTVLTRLSEEDMTLTWQNDPVTHETVLQGMGDQ
                     HIDVAVHRAQNKFQVGIVTHEPKVPYREGITRKAQAQYRHKKQSGGAGQFGEVHLRIE
                     PYMEGDFDFVDALVGMNLSRSYLPPIEKSIRATMERGAFAGYPMSNVRVIVFDGKEHE
                     VDSKPMAFEIAGREAFKLAVHDAGPVLFEPIMTVRVTCPDANMGDVMSDLNTRRGRVQ
                     GTESERGNTTIIAHVPMSEMLKYTTQIRSITGGRGYFTMEFDHYDTVPTHIAGPIIEA
                     HKKEMEAKKEE"
     gene            complement(3999..4574)
                     /locus_tag="PKI33_00440"
     CDS             complement(3999..4574)
                     /locus_tag="PKI33_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35491.1"
                     /translation="MIKRLLPAIPFIVLIACSPDISDPGVQAAVIHSLTATVWTPTPI
                     TPSPTPEPNTAKVVELLNDAMVGADPLMETIAAKYSVIDAQVIVDTTTHQAVSLQVYV
                     DCQWIYTDGCTPENTFVILVRAFSVNNKIMGKIQEQIPPTIQTLEVLTFEQMTKTSLI
                     HIAWKDICDYTEGRINGNQLGSRMIRFAVSP"
     gene            4749..5021
                     /locus_tag="PKI33_00445"
     CDS             4749..5021
                     /locus_tag="PKI33_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNM35492.1"
                     /translation="MPIVKFPPLMKFYVENQAEFEMQAKTVAELLENILVRYPILKTH
                     LFDSQGELRRHFNIFVNGVHVRELNGMETPLKEEDKVILMASAAGG"
     gene            5283..6233
                     /locus_tag="PKI33_00450"
     CDS             5283..6233
                     /locus_tag="PKI33_00450"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNM35493.1"
                     /translation="MTLLDFRLTESNTIPLDSLAYHVGNTPLLPLRRVTSNLSPRVRV
                     FAKAEWFNPGGSVKDRPALNIIQNAILNGNLSGGKRLLDSTSGNMGISYATFGAALGI
                     PVTLAVPASASAERISILKALGAELVLTDPLEGSDGAILKAREMVEEDPDKYWYANQY
                     NNEANWQAHYVSTGPEIIRQTNGQVSHFVAGLGTSGTFTGTSRYLLEQMPDVKTIAFQ
                     PDAPFHGLEGLKHMPSAIKPGIYDEALAGTPLEVKTEAAHEMVIRLAREEGLFVGISS
                     AAAAVAAIQVASELDEGVVVTVFPDAGYKYLSDKTLWEKA"
     gene            6234..6647
                     /locus_tag="PKI33_00455"
     CDS             6234..6647
                     /locus_tag="PKI33_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNM35494.1"
                     /translation="MSLKLTNEQVSEIERHIQAAYPGEGAGFLLGVDGEVKGVLPLDN
                     AREEEARYNRFLLTPEDYLKAEMKAMELGVDLIGVFHSHPDCPNVPSEYDREWAQPFF
                     SYIISRVDQGKTVSHRSWRLQEDRSKYDEEQIEVL"
     gene            6729..6965
                     /locus_tag="PKI33_00460"
     CDS             6729..6965
                     /locus_tag="PKI33_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNM35495.1"
                     /translation="MDVIKEDQVLDCTGLLCPMPIVKTKKAIDVLESGQVLKMIATDA
                     GSPPDIAAWSRQTGNELLLSTEEGGKFVFFLKKA"
     gene            6975..7499
                     /locus_tag="PKI33_00465"
     CDS             6975..7499
                     /locus_tag="PKI33_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNM35496.1"
                     /translation="MAKSDPNAPKRLALIASKGTLDWAYPPFILSTAAAAMGWEVGVF
                     FTFYGLTLLKPELTAAVSPLGNPAMPMKMPFGPENFQNISWPMPNLLMANVPGFESLA
                     TNLMKQTFKNKGVASIEELRDMAVELDVRLIGCQMTMDVFGFKKEDFIPQAEIGGAAT
                     FLEYAADADVQLFI"
BASE COUNT         2025 a         1653 c         2053 g         1991 t
ORIGIN      
        1 gcccaatatt caaagaaaga aaaatcatat tggctggcaa gttcagcaag ttttgtgccg
       61 gcggtttctg gatcaaagag tggcgcatcg gggaaaccga ggaattctcg ccagccttcg
      121 ccggtgaaat ctgcagagag ggcggtgcct gtgaagtaat ctttctcagt gaagagggaa
      181 atgcccgcat tggttagtgc caatggaata gatgaataca agcgtttgcc tgaatcaatt
      241 ccatcaaagt agcgtggagg ataggcattg agaagcgtcg cttttttgcc ggcatttaca
      301 gtctttgaaa agatggtgtg accattgaga tattgtgcca cttcagggtt gggttttggt
      361 ccgtaatggt aaccaagttc agcagggatg ttgatgcctg tgacaagaac tgcttgccct
      421 gtggctgatt ggggcagccc ttttacaccc agatttggat cgatggcttt caaagagaga
      481 acgtcgctct cgaaaggcgc agcggacttg gtcattttct tgccgccaag taacgattcg
      541 agataaggca tattggcgcg caaaaaggga ttggtgtctg ggttgtcttc gcctaagccg
      601 ataccatcaa ggaagagaaa gaggattttc ataggtaggt ttacgtatta catgatgtgt
      661 aatgcgtaaa aagccttacg aatttgcctg ccacaaaaac catgcagaga tacggtcgcg
      721 tcggtcgaag ccgtctttgc gggagacgag tgtgaggtta tggcgttgga tcaggtctag
      781 gtcagcggtg gtaattccgg gtccagtaag agccgggtct ggtttgatga atccgtagac
      841 gagccaaaat ccgcttgggt ttagcacgcg agttaactga gtcagataat cggctttgtt
      901 tgtaatactg tgaaaacagc cgaggtcgag ggctagatcg aaaggcggtg tgacttgttt
      961 catttgtgtg gcgtcgtcgg taaagacctt tactgtgaca ccggcttttt ggatttttct
     1021 tttggctatt gtgacggcac gtgaggcgaa gtcgaagcct tcggcttgcc aaccagcgtt
     1081 tgccagggtg atgaggttgg tgcctgtgcc gcaaccaata tccaaggcgc gaccggggga
     1141 gtgggctgcc atgaagtcgt ataactctgg tggagagatg ccgctatccc acggagtttt
     1201 tttagcaatg tagttgtagt tgaattttaa tcgacggaag agattattca tttgcaagag
     1261 agtcgcgcac acgctgcggg tcaaaatcgc cgaccatgcg ccaaatttcg ttgccttctg
     1321 agtcaaaata tataaatgta ggggtggcga caaaatcata aagtggcgcc agttccatgc
     1381 cgatctgttc ttggatgttt aaacgaatga tgtgaatatc ggctgggtct atttcttctt
     1441 caagttcatc cacaatgggt ttgatggata cacagcgtat acagtagggg gactggaatt
     1501 cgagcaggac gggtgtgccg gcgccgatca tgtctttcac ttgcttggca tcatccatta
     1561 agagggtttg acgcggatgc aggatcgtcc atgctacgag caacccgcct acgatgacgc
     1621 cgaatgctag ataatcgttc catttgggtt tgttcgagag caaaatgacc ccggcgacga
     1681 atgcaaaccc taccgctata aaaacaaaag aataaagccc aaaaatatcg cccattatct
     1741 agtctacaat gtcatatttt ttgtgtgacg cttcacactt tgacaattca tttaagttct
     1801 tttacaatca ctcaacctgc atcacaatct tataagaaca ggaaggtaac ttacttatga
     1861 aagagtatac caccgagttt atccgcaacg ttgcgcttgt ttcgcacggc ggagcaggga
     1921 agaccatgct tgcagaggca ttcctgcatg ttacgggggc gacgacccgt cttggaaagg
     1981 tggaagacgg gactacaata tcggattacg acgacgaaga acataaacga aaaatatcga
     2041 tttatacgag cgtgatcccg gttgaacaca agaattacaa aataaatttt cttgatgcgc
     2101 caggctacac tgattttgta ggcgagatgg tttccgcctt gagtgtggca gatggcgcaa
     2161 ttattttagt agacgctgtt gcgggcatcg aagtaggcac cgaactcgcc tggcgctatt
     2221 gcgatcaatt caatttgccg cgtttctttg tgatcaacaa aatggatcgt gaaaatgcgg
     2281 atttccaaaa aacatacgat gccatcactg aatttgtgga agcgcatgga aaacgcgttg
     2341 ttcaagttca cattcccatc ggcgagaagc aaaatttcaa aggtgtggtc gatgtcatcg
     2401 gcatgaaatc gtatatgggg gatgggaaga ccatcgccga aatccctgct gagttaaaag
     2461 aagctgcgga taaggctcac ttcgctatgg tggaagatgc cgccgaaggt gaagaccaat
     2521 tgatggaaaa atatctcgag aacggctctc tctcagacga ggaaatggtg cgtggtctcg
     2581 aagatgtggt ctacgccgga caatttgtac cggtcttttg tgcagcgggt gcgcatgaaa
     2641 ttggcaccat tgctttgctc aatgacatca ttgatttgat cccatcaccg gctcaggctg
     2701 tgaagcgtat ggcacaaggc aaagatggcg ctgaagaatt gaaagccaca gaccaaggtc
     2761 cattggctgc ttatgtttgg aagaccaccg ccgacccctt tgtcggaaag atgacctatt
     2821 tccgcgtgct ttctggctcg ctacaagcag atgcgcatgt ctggaatcaa aacaagggcg
     2881 cagatgagcg catgtctggt atgcacttcc agcgcggaaa agaaacgatt gctgccaaga
     2941 gcatccatgc gggtgatctt gcggtcgttt ccaagttgac tgtcactgcg actggcgata
     3001 ctttttgtga taaggggcat ccactcacag tggaaaaacc tgcttatcct gcagccttgt
     3061 atcgcgtggc agtagctccc aagacccaag ccgatgcggc aaagatttcc accgtgctca
     3121 cccgcttgag cgaagaagac atgaccctca cttggcaaaa cgaccctgtg actcatgaga
     3181 ccgtcttgca agggatgggg gatcagcaca tcgatgtggc agtacatcgc gctcaaaata
     3241 aattccaagt gggcatcgtt acgcatgagc cgaaggtccc atatcgcgaa gggattacga
     3301 gaaaagccca agcgcaatat cgccacaaga aacaatcagg tggtgcaggt caattcggtg
     3361 aagtacatct tcgcattgaa ccgtacatgg aaggtgattt tgattttgta gacgccttgg
     3421 ttggtatgaa cctctcgcgt tcttacttac cgcccattga aaagagcatc cgcgctacga
     3481 tggaacgagg cgcctttgct ggttacccca tgagcaatgt gcgtgtgatc gttttcgacg
     3541 gtaaggaaca tgaagtagac tcgaagccaa tggcatttga aattgctgga cgtgaagcct
     3601 tcaagctggc tgtgcatgat gctggaccgg tattgtttga gccaatcatg accgtgcgtg
     3661 tgacctgtcc ggatgcgaat atgggcgatg taatgagtga cctcaacacc cgccgtgggc
     3721 gtgtacaagg cactgaatca gaacgtggca ataccaccat cattgcgcac gtgccgatgt
     3781 ctgaaatgct caagtacact acacagatcc gctctatcac aggcgggcgt gggtatttca
     3841 ccatggagtt cgaccattac gatacggtgc cgacccacat tgcaggtccg atcatcgaag
     3901 cccataagaa agaaatggaa gccaagaagg aagaataggc aagaaagatt gaagtatgaa
     3961 gagttccggg ttttgcccgg aactcttttt gtttggaatt agggagagac tgcgaaacgg
     4021 atcatacgag accccaactg atttccgttg atgcgacctt cggtgtaatc acagatatct
     4081 ttccacgcaa tgtggatgag gctggttttc gtcatttgct caaatgtcag aacttctagc
     4141 gtttgaattg tgggaggaat ttgttcttgg attttcccca taattttgtt gttcactgag
     4201 aaagcacgca ccaagataac aaacgtattt tcgggtgtgc aaccatctgt atagatccat
     4261 tgacagtcca catagacttg taatgagact gcctggtgcg tagtggtatc gacaataact
     4321 tgagcatcaa taacactgta ttttgctgcg atcgtttcca tgagcgggtc agcaccaacc
     4381 atggcgtcat ttaaaagctc taccactttg gctgtgttcg gttcaggggt aggggatggc
     4441 gtgattggtg taggagtcca cacggttgcc gtcaacgaat ggataacggc ggcttgaact
     4501 cccgggtctg agatatctgg cgagcaggca ataaggacaa taaaggggat ggctggtaac
     4561 aaacgtttga tcatagaacc tcctttgtgc aggttttaca cccgtgccca catcaacgtc
     4621 aattccgttt gttatacgat gcacctacgc tcactcaacg atgctctcat atatataatt
     4681 tatttttaaa tgtaaatatg cagacctgcc tcttacatag tgaaaagaac ttcgtgtaga
     4741 atacaaatat gccaattgtt aaattccctc cactcatgaa gttttatgta gagaatcaag
     4801 ctgagtttga aatgcaagca aaaaccgtgg ctgagttatt ggagaatatc cttgttcgat
     4861 accccatact caagactcac ctttttgatt ctcaagggga acttcgccgg catttcaata
     4921 tttttgtgaa tggcgttcat gtgcgagagt taaatggaat ggaaacccca ttgaaagaag
     4981 aggataaggt tattttgatg gcatctgctg ctggtggatg actttccaaa ttatcttgac
     5041 aaaatcggta agattcatag aataggcgca atctaggtaa cagaaaaata tgaaacgtac
     5101 agtatattgc ttgtgtcttg ggcggggctc gtagccgccg cctgagttaa tatccgcagt
     5161 caacggaatc agacggtaga gcgacccccg cgtgcatcga atttgcatcg tgtgtggcgt
     5221 ttgttcgccg tctttttgtt tatccgttgg ctgtttttat tttaatcaga gaagagaaga
     5281 aaatgacttt attagacttt cgattaaccg aatccaatac gattccgtta gacagccttg
     5341 cttatcatgt tgggaatacg ccgcttcttc cgcttcgccg cgtgacctcg aacctgtctc
     5401 cgcgtgtgcg agtgtttgcc aaggctgaat ggttcaaccc cggcggttcg gtcaaagatc
     5461 gccctgcatt gaacattatt cagaacgcca tcttaaatgg aaatttaagc ggcggcaaac
     5521 gtttgctaga ttccacttct ggcaatatgg gtatttcgta tgcgaccttt ggtgccgcat
     5581 tgggaattcc agtcacattg gcggtgcccg ccagcgccag cgccgagcgg atttccatcc
     5641 tcaaagcctt gggcgcagag ttggttctta ccgacccgct cgaaggttca gatggagcaa
     5701 ttctcaaagc acgtgaaatg gtggaagaag atccagataa atattggtat gccaatcaat
     5761 ataacaacga agcgaattgg caggcacatt atgtgagcac gggtccagaa atcattcgcc
     5821 aaacgaatgg acaagtctct cactttgtag caggtttggg gacctctggc acgttcacgg
     5881 gtacaagccg ttatctcttg gagcagatgc ctgacgttaa gacgattgct ttccaaccgg
     5941 atgcaccctt ccacggtttg gaagggctca aacatatgcc tagtgcgatc aagcctggga
     6001 tttatgatga ggcgctcgcg ggcactccgc ttgaggttaa gacagaggcg gcgcatgaga
     6061 tggtgattcg gcttgctcgt gaagaaggtc tgttcgtggg aatctcatct gcggcggcgg
     6121 cagtggctgc gattcaagtt gccagtgaac tggatgaagg cgtagtggtg acggtcttcc
     6181 cggatgctgg gtataaatat ttgagcgata aaacactttg ggagaaagcg taaatgagtt
     6241 taaagttgac aaatgaacag gtaagcgaaa ttgaaagaca tattcaagcg gcatacccgg
     6301 gggagggcgc tggctttttg ctgggcgttg acggagaagt gaaaggggtt ttgcctttgg
     6361 ataatgcacg tgaagaagaa gcgcgctata accgtttttt acttacgccc gaagattatt
     6421 tgaaagcgga aatgaaagcg atggagttgg gtgtggattt gatcggtgtg tttcattcgc
     6481 accccgattg tccgaacgtc ccttcggagt atgaccgtga atgggcacaa ccatttttct
     6541 cttacatcat atcccgagtg gatcaaggga agacggtcag tcatcgctcg tggcgtttgc
     6601 aggaagatcg ttcaaaatac gatgaagaac aaatagaagt cctgtagggg cgacccgaca
     6661 gagtttattg ggcaccttgc tttcactggg tggggcgccc ttacaatcat atgaggagaa
     6721 tttttaacat ggacgtcatt aaggaagacc aagttttaga ttgcacggga ttattatgcc
     6781 cgatgccaat tgtgaaaaca aagaaagcga ttgatgtatt ggaatctgga caggttttga
     6841 agatgatcgc tactgatgca ggctcgccgc cagatattgc tgcatggagc cgccagaccg
     6901 ggaatgaatt gttgctttcg actgaagaag gtggcaagtt cgttttcttt ttgaagaaag
     6961 cgtagggtaa agagatggca aaatcagacc cgaatgctcc gaaacgactg gcattgatcg
     7021 ccagcaaagg caccttagac tgggcatacc cgccttttat tctttccacg gcggcagctg
     7081 ccatgggttg ggaagtgggt gtgttcttca ccttttacgg attaaccctg ctcaagcctg
     7141 aactgactgc cgcggtctct ccgcttggta accctgccat gccgatgaaa atgccgtttg
     7201 gtccagaaaa ctttcagaat atttcatggc ccatgcccaa tttgctcatg gcaaatgtgc
     7261 cgggttttga aagcttggcg accaatttga tgaagcagac ctttaagaat aaaggtgtcg
     7321 cttccattga agagttgcgt gatatggcgg tggaactgga tgtgcgattg atcggttgcc
     7381 agatgacgat ggatgttttc ggcttcaaaa aggaagattt catcccgcag gctgaaattg
     7441 gtggggcggc aactttcctt gaatatgcag ccgatgcgga tgtacaattg tttatataga
     7501 gctttttctg tcattgcgag ggcgtttttg ctctttgccc gaagcaatcc cgttgctcat
     7561 aattggattg cttcgccgcc taaaagcaag agcggcggct cgcaatgacg ggtaaatgaa
     7621 aaaaataaaa cgagagagaa actagaattc atgacaacct tgagaatccc aacccctttg
     7681 cgcacgtaca ctggtgggaa aagtgaagta accgtgagcg gc
//
LOCUS       DAOREF010000011         7591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4797_length_7591_cov_4.061173, whole genome shotgun sequence.
ACCESSION   DAOREF010000011 DAOREF010000000
VERSION     DAOREF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4797_length_7591_cov_4.061173"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKI33_00470"
     CDS             complement(<1..222)
                     /locus_tag="PKI33_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNM35497.1"
                     /translation="MNWHTIETNAALNELASSSETGLTSAQVEESKKKHGANELIERG
                     GRTPWQILWEQITATMVLILIAAAVVAGLL"
     gene            complement(507..1604)
                     /locus_tag="PKI33_00475"
     CDS             complement(507..1604)
                     /locus_tag="PKI33_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNM35498.1"
                     /translation="MKWQWKFGTFAGIDVYIHATFLLLIGWVGYSHWLEKNSWFDVLS
                     GIGFILALFLCVLLHEYGHALTARKYGIKTRDITLYPIGGVARLERMPEKPIEELWVA
                     LMGPAVNVVIAAILFAVLLLTGNLVPLTELGVASGSFLERVMVVNVSLVLFNLIPAFP
                     MDGGRVLRAILAMRMDYVKATQIAASIGQGLALLFGLIGLFGNASLLFIAFFIWIGAS
                     QEASAVQMKNVISGIPVGRAMLTDYKSLSPRDPLSRMAQLVLAGSQHDFPVIDDAERI
                     VGVVTRDDFLAALTQHGQNIAVSAVMRNNPPEVDSYDMVEVALTRIQESGFPTLPVTH
                     SGQLVGIITAENITEYLMIRTALRTAQVVSI"
     gene            complement(1809..3020)
                     /locus_tag="PKI33_00480"
     CDS             complement(1809..3020)
                     /locus_tag="PKI33_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM35499.1"
                     /translation="MDFHSIDNNSTRRIAALKHRVDTLRENNHYFYNQAVEEMLPGAK
                     VRVNGRVMGMYASYSYLGLVGHPRINEAAKRAVDKFGTGTNGVRTLAGTLTIHNELEE
                     TISNFKHAEAAITYTSGYVTNLTVISTLMGRGDYVFSDKINHASIVDGCLMSGAEFRR
                     FRHNDMEHLEGLLKNAPADVSKLVIADSVFSMDGDIIDLPKIVELSKKYNAWLMIDEA
                     HSVGVLGKTGTGIEEHFNMYGSIDIKMGTLSKTIPSVGGYVAGKKELIDYLRHGSRAY
                     IFSAALPPAQAAAANEAFKVILDEPERLDRLNANTQQFIGGLKSMGFDTLLTETAIVP
                     VLCGTDEKAMELTKRCQEQDVFVLPVVSPAVQEGMSRLRATVTAAHEPADIEHAMDVI
                     YQSGKAMGMVK"
     gene            complement(3120..5144)
                     /gene="ligA"
                     /locus_tag="PKI33_00485"
     CDS             complement(3120..5144)
                     /gene="ligA"
                     /locus_tag="PKI33_00485"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNM35500.1"
                     /translation="MADKLLSRYEELKAQINFHNYRYHVLDAPIISDLEYDRLLNELK
                     KIEAEHPDWITPDSPTQRAGAKPSEKFEKVRHPAPILSLANAFGGDDARAWFERVKKL
                     DDRVEKAKFVVEPKIDGLSVVLHYRNGMFVQGATRGDGEVGEDITSNLRTIKAIPLKI
                     PVEGQKSKVKVPSYLVVRGEAFIPIKDFEELNKRLEEAGEKTYLNPRNTAAGSLRQLD
                     PVLTASRPITLLVYQIVYAEGGDVPTSQWEILEYLKSLGFPITDISKRFNTLEPAIAY
                     TETFDKGRDALPYEADGMVIKIDDLSLAADLGFVGKDPRGAIAFKFPAREVTTTLLGI
                     DAEVGRTGVLTPRASLEPVEIGGVVVRNATLHNFDYIEEKDIRIGDRVLVKRAGEVIP
                     YVIGPVVDARSGKEKKYKPPSKCPACGQDVEHIEGEVAWYCVNAACPAQLVRNVEHFV
                     SRGAMDIVGLGIKIVEQLIEAGLVNDVADLFTLKKKDLLELEGFAEKKAENLLGSIEQ
                     AKGQSLNRLLAALGIHGVGEVMAGDLARTYGNLSALSKTTADELQQIEGVGPNIAESI
                     VDWFARPANQKLLKKLKSAGVDPQMQKDEKKKEGAFTGLTFVVTGTLPNFSRDDAKEF
                     IENNGGKVTDSVSKKTSYLVLGENPGSKFDKAKSLGVKIIDEAELKKLAS"
     assembly_gap    5190..5289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5363..5698)
                     /locus_tag="PKI33_00490"
     CDS             complement(5363..5698)
                     /locus_tag="PKI33_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35501.1"
                     /translation="MSNQLFEAALKELEARQEDVRPSDVISFPEPLRSAVNFVVRLKR
                     FSLTEFHEKLPEFTREEVKRLADILIKRHLFDLSRFATEAEPYYEARLSALTRPLTRP
                     PSDIWKKID"
     gene            complement(5728..6483)
                     /locus_tag="PKI33_00495"
     CDS             complement(5728..6483)
                     /locus_tag="PKI33_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNM35502.1"
                     /translation="MSKIISVHSFRGGTGKSNTTANIAALLAMDGARVGVVDTDIASP
                     GIHVLFNLDEADMENSLNDYLWGKCTIEQAAQDVTGHIGAEVKGQIFLIPSSIKAGEI
                     ARILREGYDVGLLNDGFRDVIEKLKLDYLLIDTHPGLNEETLLSIAISNALVIIMRPD
                     QQDYQGTAVTVDVAKKLDVPNLFILVNKAPQNLDLEEVRARVEQTYDAKVGAILPHSD
                     EMMMLASSGLFTVHYPEHVVTKGLRKLVDQLKA"
     gene            6645..7211
                     /locus_tag="PKI33_00500"
     CDS             6645..7211
                     /locus_tag="PKI33_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNM35503.1"
                     /translation="MTTNYMQNNLVSLQDASDELLIQKYLNGEREAFDALYHRHLPKV
                     YKRVRYVVPPNDVEDVTQEIFIAAVKSLSSFRGDSKFGTWLRTLTNHKVAEFYRKRTR
                     KQEPQLAPLSDAIAQVAGSTSQSMEDRIFLQRALLNLPENYQEILLLRFAEDLQFNEI
                     AELTNQNLEATKSLFRRAVTALRNHLEN"
     gene            7214..>7591
                     /locus_tag="PKI33_00505"
     CDS             7214..>7591
                     /locus_tag="PKI33_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35504.1"
                     /translation="MDQRNKSIQQDNELADFVDQLLDGKNPPASTSDEELVSLEKTLL
                     RLNDAFPPENLDEAKSKQMLARLKTRMRREEEEKAAKPSFWSRLFDLQANPQVGLLIA
                     AAVVVVFVIVSVPAVVQPPGSAVS"
BASE COUNT         1798 a         1923 c         1882 g         1888 t
ORIGIN      
        1 caacaaccct gccacaacgg cggctgcaat gagaatcaac accatggtgg cggtgatctg
       61 ttcccacaga atttgccatg gggtgcgtcc gccgcgttcg atgagctcgt tcgcgccgtg
      121 tttctttttg ctttcctcaa cctgtgcgga agtcagaccc gtttcggaag aggaagcaag
      181 ttcgttcaaa gctgcattgg tttcgattgt atgccagttc atgtattgtt tccgtgttcc
      241 taataagttt aatctatttt gatgtattta acctgacgtg ccgtttgagg aaggcgtaat
      301 actttccgca aataaaaaga ccatcgtcaa ggatgttgac gatggtcttg cctttacaga
      361 acagccggtg gcacaatgta ccacgtattg acggctgagc tacccaacat gtgggcggct
      421 actccccatc ggagttgtgc ctagtatatg gaaaaaacga ttttcgtggt taagaaatct
      481 ctcatctgca cttgcagaga ccctcattaa atcgaaacca cttgggcggt tcgcaatgcc
      541 gtgcggatca tcaaatattc ggtgatgttc tcggcagtga taatgccgac cagctgaccg
      601 gaatgagtaa ccggcaacgt ggggaagccc gactcttgaa tacgcgtcag ggcaacttcc
      661 accatatcgt atgaatccac ttcaggcgga ttgttacgca tcactgctga gacggcgatg
      721 ttttgtccgt gttgggtgag tgcggcgagg aaatcgtcgc gggtcactac gccgacgatg
      781 cgttctgcgt cgtcgatcac ggggaagtcg tgctgtgaac ctgccagaac caactgtgcc
      841 atgcgcgaga gcgggtcacg cggtgagagg cttttgtaat cggtcaacat ggcgcgtccc
      901 actggaatgc cgctgatgac atttttcatc tgcacggcgc tggcttcttg tgaagcaccg
      961 atccaaataa agaaggcgat gaataacaag ctcgcattcc cgaacaaacc gatcaacccg
     1021 aacaacaatg ccagtccctg accaatactt gcggcgatct gtgttgcttt cacatagtcc
     1081 atgcgcatgg cgaggatggc tcgcaacacc cgtccaccgt ccatggggaa ggcggggatg
     1141 aggttgaaca ataccagtga aacgttcacc accatcacgc gctcgagaaa acttccagat
     1201 gccaccccaa gttcggtgag cggcaccagg tttccggtca gtaaaagaac cgcaaaaaga
     1261 atggcggcaa tcaccacgtt aacggcgggt cccatcaacg ccacccacaa ttcctcgatc
     1321 ggtttctcag gcatgcgttc caaacgcgcc acgccaccaa tcgggtagag tgtgatatcg
     1381 cgcgtcttga ttccgtattt acgagcggtc agcgcgtgcc catattcatg caagagtacg
     1441 cataaaaaga gcgcgagaat aaagccaatg ccgctcaaca catcgaacca gctatttttt
     1501 tccaaccaat gactataccc aacccacccg atcaaaagta aaaaggtggc atggatgtag
     1561 acatcaattc ctgcaaatgt tccaaatttc cattgccatt tcatagtttg ctccttgtaa
     1621 cgttgacggc attgtacaag gaatttatta gaaaaatgtt ataaaaattc tatcaagaac
     1681 tatcaggatg aaaaccccgc ttcccactcc gcttgggctt ggtctgtctc tccaagcagc
     1741 agcgaacgcc gataaaaaaa tcgcctgtcc tatcgacagg cgattttcaa ctttatctaa
     1801 ctatctaatt acttgaccat tcccatggct ttgccagatt ggtagatcac atccatggcg
     1861 tgttcaatat ccgcgggttc gtgtgccgca gtgaccgtgg cacgcaagcg agacatgccc
     1921 tcttgcactg caggcgagac gacgggcaga acgaagacat cctgctcttg gcagcgtttg
     1981 gtcaattcca tggctttttc atcggtgccg cacagcacag ggacgatggc agtttcggtg
     2041 agcaaggtat caaagcccat gctcttcaag ccaccaataa attgttgagt gttggcgttc
     2101 aagcggtcga ggcgttcggg ttcatcgaga atgaccttga aggcttcgtt cgcggcggct
     2161 gcctgagcag gtgggagcgc tgccgagaag atgtacgcac ggctgccgtg gcgcaggtaa
     2221 tcgatcagtt cttttttgcc agccacatat ccgccaaccg aagggatggt cttgctgagg
     2281 gtgcccatct tgatgtcgat ggagccgtac atgttgaagt gttcttcaat accggtgccg
     2341 gtcttgccga gcacgcccac cgagtgcgct tcgtcgatca tgagccaggc gttgtatttt
     2401 ttgctaagct ccacgatctt gggcaggtcg atgatatcgc cgtccatgct gaagaccgag
     2461 tccgcgatga cgagcttgga gacatcggca ggagcgttct tgagcaagcc ttcgagatgt
     2521 tccatgtcgt tgtggcggaa acggcggaat tccgcaccag acatcaaaca accgtccacg
     2581 atggaggcgt ggttgatctt gtctgagaag acgtagtcgc cgcgtcccat caaggtagag
     2641 atgacggtca ggttggtgac atacccagaa gtataggtaa tggcggcttc tgcatgcttg
     2701 aagtttgaaa tggtttcttc gagttcgttg tgaatagtga gggttccagc caaggtgcgc
     2761 acaccgttgg tgccggtgcc gaatttgtct acggcgcgtt tggcggcttc gttaatgcgt
     2821 gggtgaccga ccaagcccaa gtaggagtaa gaggcgtaca tgcccatcac gcgcccattt
     2881 acgcgcactt tggcgccggg gagcatttct tccacggctt ggttgtaaaa ataatgattg
     2941 ttttcgcgta gggtgtctac acggtgtttg agagcggcaa tgcgccgggt gctgttattg
     3001 tcaatgctat gaaaatccat gaaagagcct ccaaaaggga tggcgcaagt ttagccgagg
     3061 gggtgttttc tgtctagctt aaaagtgaca gtcaccttta aggtgactgt cacttgtgtt
     3121 tagcttgcta attttttcaa ttcggcttcg tcaatgattt ttacccccaa agacttcgcc
     3181 ttatcgaact tggagccggg gttttcgcca agcaccaaat agctggtttt cttgcttaca
     3241 ctatcggtca ctttaccgcc gttgttctca atgaattctt tggcatcgtc acgcgagaaa
     3301 tttggcaggg ttcccgtcac cacaaaggtc aagccggtga aggcgccttc ttttttcttt
     3361 tcgtcctttt gcatttgcgg gtctacgcca gccgacttta acttcttcaa taatttttgg
     3421 tttgccgggc gagcgaacca atccacaata gattcggcga tgttcggacc cacgccttcg
     3481 atctgctgaa gttcatcggc agttgtcttt gataatgcgg atagatttcc atatgtgcgt
     3541 gccaaatcgc cagccatgac ttcgcccaca ccgtggattc ctaacgcagc gagcaagcgg
     3601 ttcaaactct gaccctttgc ctgctcaatc gacccgagca gattctccgc ctttttctcg
     3661 gcaaagcctt ccaactcaag caggtctttc ttctttagtg tgaacaggtc cgctacatca
     3721 ttgaccagac cggcttcgat taattgttcc acgatcttga tgcccaagcc gacaatatcc
     3781 atcgcgccgc gcgagacgaa atgctccacg ttgcggacta actgtgccgg gcaagcggca
     3841 ttgacgcagt accaagccac ttcgccttcg atgtgttcta catcttgccc acatgccggg
     3901 cattttgaag gcggcttgta tttcttctct ttgcccgagc gcgcatctac gaccggaccg
     3961 atgacatacg gaatcacttc acccgcgcgt ttgaccagga cccggtctcc gatgcggatg
     4021 tctttctctt cgatgtaatc aaagttgtgc aaggttgcat tgcgaacgac caccccgcca
     4081 atttccaccg gctccaacga ggcacgcgga gtcaacacac cggtgcgccc cacttcggcg
     4141 tcaatgccga gtagggtggt ggtcacttcg cgggctggga acttgaaggc aatggctccg
     4201 cgcgggtctt tgccgacaaa gcccaagtct gcggcgaggg ataaatcgtc gatttttatg
     4261 accatgccat cggcttcgta aggaagcgcg tctcgtcctt tgtcaaaggt ttcggtatag
     4321 gcgatggctg gctccaaggt gttgaagcgt ttggaaatat ccgtgatggg aaaccccagc
     4381 gatttgagat attccaaaat ttcccattgc gaggtgggga catcaccccc ttcggcgtac
     4441 acgatctgat acacaagcaa tgtgatcggg cgagatgcag tcaacaccgg gtcaagctga
     4501 cgcagcgacc cagccgccgt gttgcgcgga ttgagatagg ttttttcgcc agcttcttcg
     4561 agacgtttgt ttaattcttc aaagtctttg atcgggatga atgcctcgcc gcgtacgaca
     4621 agataggaag gtaccttaac tttggacttt tgcccttcca ccggaatttt tagtggaatg
     4681 gctttaatag tgcgcaagtt gctggtgata tcttccccta cttcgccgtc gccgcgggtg
     4741 gctccctgca cgaacatgcc attgcgataa tgcagcacaa ccgacaagcc gtcgatcttc
     4801 ggctcaacca cgaactttgc cttttctaca cggtcatcta attttttgac gcgttcaaac
     4861 caggctcgtg cgtcatcacc gccaaaggca ttggcaagtg aaaggatggg tgccgggtgg
     4921 cgcaccttct caaatttttc tgaaggcttc gccccggcac gttgagtggg ggagtcggga
     4981 gtgatccaat cggggtgttc tgcctcgatc ttttttagtt cgttgagtag gcggtcatat
     5041 tcgaggtcac tgataatagg cgcatccaac acgtgatacc gataattatg gaagttgatc
     5101 tgcgctttaa gttcttcata tcgtgagaga agtttatctg ccattttttg attataccca
     5161 gtgcagagaa aaataaaacc cgcctgtttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnt atctgccatt ttttgattat acccagtgca gagaaaaata aaacccgcct
     5341 gtttatgcag gcgggcgaga gatcaatcga ttttcttcca aatgtctgat ggcgggcggg
     5401 tcagcggacg ggtcaatgca gagaggcgag cttcgtagta gggttccgct tcggtggcaa
     5461 accgtgaaag gtcaaacagg tggcgcttga tcaaaatatc tgccaggcgt ttcacttctt
     5521 cacgggtaaa ttctggcagc ttttcgtgaa attccgtcag gctgaagcgt ttcaaccgca
     5581 cgacgaaatt cactgcagaa cgcagcggtt cagggaacga gatcacgtct gaaggacgaa
     5641 catcttcctg acgtgcttcc aattccttaa gcgcagcttc aaaaagttgg ttgctcatgg
     5701 cgccttccct tcacctaggc gaatgcccta ggctttgagt tgatccacca gtttgcgcaa
     5761 gcctttggtc accacatgtt caggataatg aacggtgaag agaccagaac tggcgagcat
     5821 catcatttca tctgagtgcg ggagaatagc tcccactttc gcgtcgtagg tctgctccac
     5881 gcgggcgcgg acctcttcaa ggtccagatt ctgaggcgct ttgttgacga gaataaagag
     5941 gttgggaaca tcgagcttct tcgcaacatc cacggtcact gctgtgccct gataatcttg
     6001 ctggtctggg cgcatgatga tgaccaaggc gttggagatg gcaatggaga gcaaggtctc
     6061 ttcgtttaag ccggggtgag tatcaataag aagataatcc agcttcaact tttcgatcac
     6121 atcgcggaag ccgtcgttca acagacccac gtcgtaacct tcacgcaaaa tgcgggcgat
     6181 ttcgcctgct ttgatgctgg aggggatgag gaaaatctga cccttaacct cggcgccgat
     6241 atgtccagtc acgtcttgag cggcttgttc gatggtgcac ttaccccaca aatagtcatt
     6301 gagggagttt tccatatccg cttcatctaa attgaagaga acatgaatac cgggcgaggc
     6361 aatgtcagta tccactacac caacacgtgc accatccatg gcgagcaaag cagcaatatt
     6421 tgctgtggta ttcgatttac ccgtgccgcc gcggaaggaa tgaactgaaa tgattttgga
     6481 catgcactgt ctccgattct atgatagtct ttcgaaatct ctgaaaatca gttcgataat
     6541 tatagcctaa ctatattgaa aatcaaatcc ctacttttca tttctcagtt atgttatagt
     6601 acctttctaa actgaaagac gaaaacgacc cattttggcg cttcatgacg acaaattata
     6661 tgcaaaacaa cctagtatcc ctgcaggacg ccagcgacga acttctcatc cagaaatacc
     6721 ttaatggaga acgagaagct ttcgatgctc tttatcaccg ccacctcccc aaggtctaca
     6781 aacgggtcag gtatgtggtt ccgcccaacg atgtagagga tgtgacgcag gagatcttta
     6841 ttgctgcggt aaagtctctc tcgtcctttc gaggtgactc gaaatttggc acatggctgc
     6901 gcacccttac caaccacaaa gtggcagagt tctatcgaaa acgcacccgc aagcaggagc
     6961 cgcaacttgc accattgtca gacgcaattg cccaagtcgc tggtagcacg tcccaatcga
     7021 tggaagatcg catcttcctc caacgagcgc tcctaaacct gccagagaac tatcaggaaa
     7081 tcttgctgct gcgttttgcc gaagatctac agttcaacga gattgctgaa ttgaccaacc
     7141 aaaatcttga agctaccaaa tctttgtttc gtcgagccgt taccgcactt cgcaaccacc
     7201 tggaaaatta accatggatc aaagaaacaa atctatacaa caagataacg aactcgcgga
     7261 ctttgtcgac caattgttgg atgggaaaaa ccctcccgcc tcaacctcag acgaggaact
     7321 ggtcagcctc gagaaaacgc ttctccgttt gaacgatgcc ttccctccag aaaacctgga
     7381 cgaagcaaaa tccaagcaaa tgcttgcccg cctaaagact cgtatgcgcc gggaagaaga
     7441 ggaaaaagca gccaagcctt ctttttggag caggcttttt gatcttcaag ccaaccccca
     7501 agtcggtttg ttgatcgcag cagcagtagt ggttgttttt gtgatcgtta gtgttcctgc
     7561 tgtggttcaa cccccaggtt ctgccgtctc t
//
LOCUS       DAOREF010000012         7539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4864_length_7539_cov_3.653394, whole genome shotgun sequence.
ACCESSION   DAOREF010000012 DAOREF010000000
VERSION     DAOREF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4864_length_7539_cov_3.653394"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKI33_00510"
     CDS             complement(<1..513)
                     /locus_tag="PKI33_00510"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNM35505.1"
                     /translation="MKKRVYIIYTGGTIGMQPTSEGYAPAPGYLAEQIMSMPELKSDL
                     MPDVEIHEYDPLLDSANMTPEDWYKMARDIGSHYDEYDGFIILHGTDTMAYTASALPF
                     MLEGLGKPVVLTGSQIPLCEVRNDARANIITALTVAANFDIPEVCLCFGNQLLRGCRS
                     VKVSTDGLDAF"
     gene            complement(550..1308)
                     /locus_tag="PKI33_00515"
     CDS             complement(550..1308)
                     /locus_tag="PKI33_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HNM35506.1"
                     /translation="MSFTSHPEVDQVLSTLLENVQGILGHYFVGMYLYGSLATGDFNP
                     KSSDIDFCVVIQDTLPDEFIAHLEMMHKKIWASPFKWAAKLEGAYLPKSLIRRHTPNG
                     PACPVVNEGKFYLGTQGSDWVIQRHVLREKGVVLAGPDPITLIDYVSPEEIRNAVRGV
                     LEEWWFPMLTDSAWLVNHGSEYHAYAVISMCRALHALEHGTIVSKPIAAEWASIQYAD
                     HARLIKKSLDAQDGTSAGFAEDALGFIDMVKKLV"
     gene            complement(1311..2936)
                     /locus_tag="PKI33_00520"
     CDS             complement(1311..2936)
                     /locus_tag="PKI33_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNM35507.1"
                     /translation="MLSIHNLNKHFGIQPVLKNINFNISEGERIGLIGANGSGKTTLM
                     RILAGFEHPDSGDVASTRPNLRLGYLAQGMDFSPEQTLHSALGLDSDSQTDPALEVES
                     LALALAQNPNDAALQAQYDLALHKLSGESIQPSAVLEPLGLSHFPADTPIAHLSGGQK
                     TRLMLAKVLLDDPQLLLLDEPTNHLDIDMLEWLEDWLNGFKGAALIVSHDRTFLDNAV
                     TKVLELDSSTHTIKTYDGNYSDYLEQKLLEFDKQMQAYQDQQDEIAQLKRAARQIRSL
                     TVMKKGGKSDSGDKFAKGFFGNRATKNVAGRAKNLEARIDHLLTDEKVDKPQGSWKLK
                     LDFGAPEHQSKDVLVTDNLSIGYAPETPLLTNINLFIRAGQRVVLTGANGSGKTTFIR
                     TIVGKLHPLTGSFRLGGATKLGYMAQEQELLNPALNSVQTIQSVSAINETETRNFLHY
                     FLFKGDAALRPAGDLSFGERARLQLALLVAQGCTFLILDEPINHLDIPSRERFEEALE
                     NFNGTILAVVHDRSFIERFATDVWQAKDGKILK"
     gene            3156..4001
                     /locus_tag="PKI33_00525"
     CDS             3156..4001
                     /locus_tag="PKI33_00525"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNM35508.1"
                     /translation="MTKKVLAVLAHPDDESFGLGGTLALYAQRGYETYYVCATRGEVG
                     TVSPEFLKGFKDTAEMRTDELMRAAKELGLKEVIFLDYRDSGMPGMEANQHPNAQINH
                     PVEEVAGKVVKHIRAIKPDVVITFDPIGGYKHPDHIHIHKATVLAFANADDENFHPEA
                     GEGFKPSALYFQVFPRWFLRVMTNLMPLIGKDPTKWGRNGDINLKELAEVDFPVHVRI
                     NTRSVAEIKTRASAQHASQGGTQMRRGLMGFVTRLFGEREDFMQAYPPVEGEFKRKSD
                     LFEGI"
     gene            4110..4859
                     /locus_tag="PKI33_00530"
     CDS             4110..4859
                     /locus_tag="PKI33_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNM35509.1"
                     /translation="MTQSEPIKPRFQFSRISEVLLQPQHTFALLVSENVGWRMPMLVL
                     TVSTTLVGIVSGFLKARAASMGEVTLPPDWQWWTPDMQNNYMQAQQSMQGPVFTYVLP
                     LVGALLGLWLGWLILGALLHFGSTMFGGRGSMQSALMITAWASLPFLVRDGLRIIYML
                     IAQHPIQSPGLSGFTTNAVFASQLLSKVDLFYIWSIVLLVIGFGVTDNMPRNKSISNV
                     VIVSILLLLIQSGVGSLLSGVSGLAIQRPFF"
     gene            4861..5589
                     /locus_tag="PKI33_00535"
     CDS             4861..5589
                     /locus_tag="PKI33_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM35510.1"
                     /translation="MNSSFIRVVGLKRHYEMGGTLVRALDGVDLEIPSHTFTVVMGPS
                     GSGKSSLLYLLGGLDRPTAGEIYVNGERLDQMDENALALFRRKTMGFIFQSFNLVSSM
                     TALENVAFPMQFAGVPVSQRNEKSQSLLSQVGLAERSHHKPSELSGGQQQRVAVARAL
                     VNDPVLIFADEPTGNLDTQSGVTIMQMLSDLHKSGRTVLVVTHDPRMVRFATNKIFLL
                     DGKVVTEAEYQAVALEPLQGNSGG"
     gene            5590..7299
                     /locus_tag="PKI33_00540"
     CDS             5590..7299
                     /locus_tag="PKI33_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNM35511.1"
                     /translation="MNLVRKSFLWVGVIMALLVSSIPTSVLAADLLSVTPTSVVNTAD
                     KTITVTGTGFDNTAVVLLDGSALATTFYNDQTLTAVVPAGYVPKTYNLTVTMSGGTVN
                     IPTPVSFTVTAPAPTNTPIPTPTAAPFARPQLRVSFSGTSGKDGIVSNKPFKFTVNFE
                     NAGSLPAYNTQATFTSADLVPLDTGGVAVLGSVSAGSSVSVDQRFVSNGSLSGKSIIT
                     IDVTLSYNDEKGNAYSDKFTLSASVSGGGGGSGVYYTATPTGVRSGQLIITSYGTDVD
                     PLEPGSRFNLVMTVQNMGNEKAPRVTMIVGGGSSGSSDGTPQPGGVSGGNGEFTNFAP
                     VGTSNIQSLGDLPAGGMVQAKQDLIVNVSTEPGAYPIKVTFSYLNAKGEVVNDEQVIT
                     LLVYSLPNLDISFYRPLDPFFVDQPGALPIQVVNLGKRTAVLGTITVTTDNGTVDGGT
                     SLVGSLDKGGYYTLDATLTPLQTGPAKINFVIEYTDDFNQKRTVEKTIDVNVEEGFIE
                     PTPDPNVPLDTGGMTPPTEETFLQKTWRFILGLLGLDSAPSTSSPDMQSPPTDFQSVP
                     SGG"
     gene            7321..>7539
                     /locus_tag="PKI33_00545"
     CDS             7321..>7539
                     /locus_tag="PKI33_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35512.1"
                     /translation="MKFSDLVRLVINNLSRRKARVALTAIGVVIGTGAVVILVSLAIG
                     LQMNANEQLYGIGDLSQISVVPAYGGGPM"
BASE COUNT         1872 a         1736 c         1875 g         2056 t
ORIGIN      
        1 gaaggcatcc aagccatcgg tgctaacctt caccgagcgg catccgcgta ggagttgatt
       61 gccaaaacac aaacaaactt cggggatatc aaaatttgca gcgacggtca acgcggtgat
      121 gatgttcgca cgcgcatcat tgcgaacttc gcacaacgga atctgcgaac cggtaaggac
      181 aacaggtttt cccaatcctt ccaacatgaa tggcaaagcg gaggcagtgt atgccattgt
      241 gtcagtgcca tgcaaaatga tgaaaccatc gtactcatcg tagtgactgc caatatcgcg
      301 ggccatcttg taccaatctt cgggggtcat gttcgccgag tcgagcagtg ggtcatactc
      361 atgaatctcc acatcgggca tcaggtcgct tttgagctct ggcatggaca tgatctgctc
      421 tgccaaatat ccgggcgcgg gggcatagcc ttccgatgta ggctgcatgc caatggtccc
      481 gccggtgtag atgatgtata cgcgtttttt catgaaatat tcccaaggtt aagttaattg
      541 atttttacat taaaccagct tcttgaccat atcaataaaa cccagtgcat cctcggcaaa
      601 gcctgcgctt gttccatctt gtgcatccag agattttttg atcaagcgag catgatctgc
      661 gtattgaatc gatgcccatt ctgccgcaat gggtttcgaa acaatcgttc catgctcaag
      721 ggcgtgtaat gctctgcaca tggaaatgac ggcatacgca tggtattcac ttccatggtt
      781 cactaaccat gctgaatcag tgagcatggg aaaccaccat tcttccaaaa cacctctcac
      841 agcgttccga atctcttcag gcgatacgta atcgatcaga gtgattgggt ctggacctgc
      901 aagcacaacc cctttctcgc gaagtacatg ccgttgaatg acccagtcac tgccttgcgt
      961 accgagataa aacttgccct cgttgacaac agggcaggca ggtccgttcg gggtgtggcg
     1021 acggataaga cttttgggga gatacgcacc ctccaattta gccgcccatt tgaatggact
     1081 tgcccaaatc tttttgtgca tcatttctaa atgagcaata aattcatcag gcaaagtatc
     1141 ttggatgacg acacaaaaat caatatcact gcttttgggg ttgaaatctc cagttgccag
     1201 tgaaccatag agatacatgc caacgaaata atgccctaaa ataccctgca cattttcgag
     1261 cagggtactg aggacctggt ctacctctgg atgcgatgta aaagacatcc tcatttcaaa
     1321 atcttcccgt cttttgcctg ccatacatcc gtagcaaagc gctcgataaa actgcgatca
     1381 tggacgacag cgaggatggt tccgttgaaa ttttccaagg cttcttcgaa acgttcacga
     1441 gaaggaatat caagatggtt gataggttcg tccaagatta ggaaggtaca tccctgtgcg
     1501 acgagtaatg ccagttgtaa ccgggcacgc tcaccaaaag aaaggtcacc ggctgggcgg
     1561 agcgcggcat ctcctttgaa gagaaagtaa tgcaggaagt tgcgcgtctc ggtctcgttg
     1621 atagctgaga cactttgtat ggtttgcacc gagttcaatg ccgggttgag cagttcctgt
     1681 tcctgtgcca tatagccaag tttggtcgcg ccacccagcc gaaacgaacc tgtcaacgga
     1741 tggagtttgc caacgatcgt acgaatgaag gttgtcttcc ctgagccgtt tgcacccgtc
     1801 aacaccacac gctgaccagc gcggataaat aagttgatgt ttgtcagcaa cggggtttca
     1861 ggtgcgtagc caatggagag gttgtcagtc accaacacat cttttgactg atgctccggt
     1921 gcgccaaaat ccaatttcaa tttccatgag ccttgcggct tgtcaacttt ttcatcggtt
     1981 aacaaatggt cgatgcgggc ttcgaggttc ttggctcgcc cagctacatt ttttgtagcg
     2041 cggttgccga aaaagccttt cgcaaattta tcgccactgt ccgatttgcc gcctttcttc
     2101 atcactgtca aactgcggat ctgtcttgcc gcgcgtttca gttgcgcgat ctcgtcctgc
     2161 tggtcttgat aggcttgcat ctgcttgtcg aactcaagca gtttttgttc aagataatca
     2221 ctgtaattgc cgtcatatgt tttgatggta tgagtcgaag aatccaattc gagcaccttg
     2281 gttacagcgt tatctaaaaa tgtgcggtca tgcgagacga tcaatgccgc acctttgaat
     2341 ccgttcagcc aatcttcgag ccattcgagc atgtcgatat cgaggtgatt ggtgggttcg
     2401 tccagcagta gaagttgcgg atcatccaac aaaactttgg caagcatgag acgggtcttt
     2461 tgcccgccag agagatgggc gatgggcgta tcggcaggga aatgggacaa acccaacggc
     2521 tccaacaccg ccgatggctg gatgctctct cctgaaagtt tgtgcaacgc taaatcatat
     2581 tgtgcttgaa gcgcggcatc attcgggttc tgagctaatg ccaaagcaag cgactccact
     2641 tccagcgcgg ggtcggtttg ggaatctgaa tccagcccaa gcgcggagtg aagcgtttgt
     2701 tcgggggaga agtccatgcc ttgggcgaga tagccgaggc gtagattcgg gcgcgtcgaa
     2761 gcgacatccc cagaatctgg atgttcgaac ccggcaagga tgcgcatgag cgtggtcttg
     2821 ccgctgccat ttgcgccgat gagcccaatg cgttcgcctt cgctgatatt gaagttgatg
     2881 tttttgagaa cgggctggat accgaaatgt ttgttgaggt tatggatact aagcatatga
     2941 acgaactcct gtagttaaaa agaaaaccga gggcaagcct cggcaatatt aaacgccaat
     3001 agggaaatgc cctgaaaaaa atggtttaac tacttgctgt ccgtcattcg ctgggtatcc
     3061 tttcttgaga aaaattttat cacattccgt aaagcagggg taaacttgtg gatagtttga
     3121 acgtcaaaaa cgcatcattg atcaaagagg ttaacatgac gaagaaggtt ttagcagtac
     3181 tggcgcaccc cgacgacgag tcgtttggct tgggcgggac attggcgctt tatgcacaac
     3241 gcgggtatga aacatattat gtttgtgcca cacgcggcga agttggcact gtttctccag
     3301 agttcttaaa gggatttaaa gataccgccg aaatgcgcac cgatgaattg atgcgtgccg
     3361 ccaaagaact tggtttgaaa gaagtcatct ttcttgatta tcgtgattcg ggtatgcccg
     3421 gcatggaagc taaccaacac ccgaatgccc agatcaatca tccggtagag gaagtggcag
     3481 gcaaggtggt gaagcatatc cgcgccatta agccagatgt tgtgattacg ttcgacccga
     3541 ttggcgggta taagcacccg gatcatattc atattcacaa agctacggtc ctcgcttttg
     3601 ccaatgccga tgatgagaat ttccaccctg aagcaggaga aggtttcaag ccaagtgctt
     3661 tgtatttcca ggtctttcca cgctggttct tacgtgtgat gaccaacctt atgcccctga
     3721 tcggtaagga ccccaccaag tggggtcgca atggcgacat caatttgaaa gaacttgctg
     3781 aagtagattt ccctgtgcat gtgcgcatca atacccgttc tgtggctgag atcaagactc
     3841 gcgccagcgc tcaacatgca tctcaaggcg gcactcaaat gcgccgcggt ttgatgggtt
     3901 ttgtgacacg tttgttcggc gagcgagaag attttatgca agcatatccg ccagtggaag
     3961 gcgaattcaa acgaaaaagt gatttgttcg aaggaatata gaaaatgaat gaagcggctg
     4021 gaacaaatcc agccgctttt tcgttaatcg ctattaagca tgaaaatttg gtgcatgtta
     4081 gaatttttac gaggaaaaac ggaatatata tgacccaatc tgaacccatt aagccacgtt
     4141 ttcagttttc tcgtatttct gaagttttgt tacagcctca acacaccttt gccttattag
     4201 tcagcgaaaa tgtgggttgg agaatgccta tgctggtgct gacggtttca accacactgg
     4261 ttggcattgt tagcggattt ctgaaagccc gcgctgcttc catgggagaa gtgacgcttc
     4321 cgccggattg gcaatggtgg acgcccgata tgcagaataa ttacatgcag gcacaacaat
     4381 ccatgcaggg accggtcttt acctacgttc taccgctggt tggggcgttg cttggtctgt
     4441 ggcttggttg gctgatttta ggtgccttgc tccattttgg ctcgacaatg tttggtgggc
     4501 gaggctcaat gcaaagcgcc ttgatgatca cagcatgggc aagcctgcca tttctggtac
     4561 gagatggctt gcgtattatc tatatgctca ttgcgcaaca tcctattcaa agccctgggc
     4621 tttctgggtt tacgaccaat gcagtatttg cttctcaact actttcaaaa gtcgacttgt
     4681 tttatatttg gagcatcgtt cttttggtca tcggctttgg cgttactgat aacatgccgc
     4741 gcaataaatc aatatcgaat gttgtaattg tttcaatctt gcttcttctc attcaatctg
     4801 gcgttggttc tttattgtca ggggtgagcg gtttggcgat tcaacgtcca ttcttttaaa
     4861 atgaattctt cttttattcg agttgttggt ttaaaacgac actatgaaat gggtggaacc
     4921 cttgtccgcg ccttggatgg cgtggattta gagattccat ctcacacctt tacagtggtt
     4981 atgggacctt ccggctcagg caaaagtagt ttgttgtatc tgcttggtgg gttagaccgc
     5041 ccaactgcgg gtgaaattta cgtcaacggc gaacgtttgg atcaaatgga cgagaacgcg
     5101 cttgcacttt ttcgccgaaa aacgatgggt tttatttttc aatcgttcaa ccttgtttca
     5161 agcatgacgg cattagagaa cgtggctttc ccaatgcaat ttgcaggcgt gcctgtttca
     5221 cagcgaaatg aaaaatccca atccctctta tctcaagttg ggctggcaga gcggagtcac
     5281 cataaaccct ctgagctttc aggcgggcaa caacagcgtg tggctgttgc acgggctttg
     5341 gtaaatgacc ctgttttgat ctttgcagat gagcctactg gcaacctaga tactcaaagc
     5401 ggtgtgacca ttatgcaaat gctctcagac ctgcataaat ctggtcggac tgtgctggtg
     5461 gtaacgcacg atccacgcat ggtgcgtttt gcgaccaata agatattttt gttagatgga
     5521 aaagtggtta cagaggctga gtatcaggct gttgcactag aaccgcttca ggggaattct
     5581 ggaggataaa tgaatttagt aagaaaatcc tttttgtggg tcggcgtgat tatggctctt
     5641 ttggtttcaa gtattccaac ctctgttttg gctgcggatt tgttgtctgt aacgccaaca
     5701 tctgttgtga atactgccga taagacaatt acagtaacag gaactggatt tgacaacacg
     5761 gctgttgtat tgcttgacgg ctcagcttta gcgactactt tttataatga ccagactttg
     5821 acggcggttg tccctgctgg gtatgtgccg aaaacatata accttacggt gacgatgtct
     5881 ggtggaactg ttaatatccc tacacccgtt tcttttacag ttacagcacc ggcaccaaca
     5941 aatacaccaa ttccaacacc aaccgctgca ccatttgcac gccctcagtt acgtgtatct
     6001 ttttctggta caagcggtaa agatggcatt gtttccaaca aaccatttaa gttcactgta
     6061 aattttgaaa atgcaggctc tttgccggca tacaataccc aggcaacctt tacatctgca
     6121 gaccttgtcc ctttggatac gggcggtgta gcggttcttg gaagtgtctc tgcgggttct
     6181 agcgtatcgg tcgaccagcg ctttgtttcc aatgggtctt tatctggaaa gtctattatt
     6241 acgattgatg tcacattgag ctacaatgac gagaaaggca atgcctattc tgacaagttc
     6301 acactttctg cttctgtatc cggcgggggc ggcggatcag gcgtctatta cacagccact
     6361 ccaaccggag tccgaagcgg gcagttaatc attacttcat atggcacaga tgtggatccg
     6421 cttgagccgg ggtctcgatt taatctggtg atgaccgttc aaaatatggg taacgaaaaa
     6481 gcgccgcgcg ttacgatgat cgttggtgga ggttctagcg gttctagcga tgggactccg
     6541 caaccaggcg gtgtttctgg tggaaatggg gagttcacga attttgcgcc ggtagggaca
     6601 tcgaatatcc aatcgttagg agatttacct gccggaggca tggttcaagc caagcaagac
     6661 ctgatcgtca atgtttcaac tgaaccgggt gcatatccta tcaaagtcac attctcttat
     6721 ctgaatgcca agggtgaggt ggttaatgat gagcaggtga ttactttatt agtttacagc
     6781 ttgcccaatc ttgatattag tttctatcgc ccgcttgatc cattttttgt tgaccaaccc
     6841 ggcgcgttgc caattcaagt tgttaacttg gggaaacgca ccgctgtgct tggcaccatc
     6901 acggtcacca cagataatgg cacagtggat ggcggtacat ctttggtcgg ttcattggat
     6961 aagggcggat attacacctt ggacgcgacc ttaactccgc tgcaaaccgg acccgcaaaa
     7021 ataaattttg ttattgaata tacagacgac tttaatcaaa aacgtaccgt tgagaaaaca
     7081 atcgatgtta atgttgaaga aggctttatt gagccaacac ctgacccgaa cgttcctctg
     7141 gatacgggtg gaatgactcc gccaactgaa gagacatttc tacaaaaaac atggcgcttc
     7201 attcttggac ttttaggttt agacagcgct ccgtcaactt cttctcccga tatgcagtct
     7261 ccacctacag actttcaatc cgtgccgagt ggcggataag atgatgaatc tggttaacac
     7321 atgaaattct cagatctagt ccgtttggtc atcaataact tgagccgtcg caaagcgcgc
     7381 gtggccctta ctgcgatcgg tgttgtcatt ggcacaggcg cggttgtgat ccttgtgtct
     7441 ctagctattg gcttgcagat gaatgctaat gaacagttat atgggattgg tgaccttagc
     7501 cagattagtg tcgttccggc ttatggggga ggacccatg
//
LOCUS       DAOREF010000013         7456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_4947_length_7456_cov_3.716390, whole genome shotgun sequence.
ACCESSION   DAOREF010000013 DAOREF010000000
VERSION     DAOREF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4947_length_7456_cov_3.716390"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKI33_00550"
     CDS             <1..978
                     /locus_tag="PKI33_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35513.1"
                     /translation="PDEIASQISALEPVKAATPRLYASGFLSTGDESTGVQINGIDPQ
                     SVANDPYRNGIISGEFLNADDREGLLIGRPAAEKLHLKVGDKVSLSVNTADGNVQEQV
                     FIIRGIYTTDTYGFDSGTVFLPLAKAQAITQTEGHASTIFVLLNDTADTDQVVAALKS
                     SSSLEVLTWRDLNKLFVEFETMAQSYISFFYLIILAITASVIVNTLIMYVYERTREIG
                     ILSAIGMRGSRILGLFLAESFMLAVGGIVMGLALGVFAVYLFNINGFFVGNMGLSGML
                     VSDTIRAQLTVDNTINVTVMTFIATILSGLYPAVMASRLEPVQALRAEK"
     gene            1008..1691
                     /locus_tag="PKI33_00555"
     CDS             1008..1691
                     /locus_tag="PKI33_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM35514.1"
                     /translation="MEVVKIENVTRTYQIGKLETHALRGVSLSINSGEFTALVGPSGS
                     GKTTLLQMIGCLDQPTSGRVTVDGKEVTSLNRNQRADMRKGHIGFIFQFFALIPTLTA
                     YENVEMPLLLNGHSPKERRDRVMELLKAVDITDRANNRPDQLSGGQQQRIAIARALAP
                     KPALILADEPTANLDTENGKQVMEIMKKLNQETGETFVFATHDPRVINYASRVVTLED
                     GVIVKDSKG"
     gene            1780..2613
                     /locus_tag="PKI33_00560"
     CDS             1780..2613
                     /locus_tag="PKI33_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNM35515.1"
                     /translation="MKRVIFVVVSLLALLLSACGQASSTEAVPAVVIDAGNSSQPADS
                     QPSSGGSVSASAIVVPMQDANLSFTNIGRVTQVNVQVGDKVSAGDVLVQLDTTILEAK
                     VREAEANLKYAQISLDYLIRNVGCKMNCAPSQEHIEVAENDVAKAQALVDSAKAVLAA
                     QSNLTAPFAGTVISVDIAPYETVAPGQVVMVIGDLSKYQIETTDLSERDVTQVKVGQS
                     ASVYIEALDEEFTGKVVDVARISSEVGGDVVYKVTIEFDKQPAGLLWGMSADVNIDAG
                     E"
     gene            2621..3448
                     /locus_tag="PKI33_00565"
     CDS             2621..3448
                     /locus_tag="PKI33_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNM35516.1"
                     /translation="MKRLLILTVGLLVLTACASPTPTPVPDAVETTAPVIEVSSPDVV
                     IASADVEPTHVSHLGFTISALVKEVAVGEGDVVKAGDTLMVLNTPDLQYAVVSAEADF
                     NARTQAAQLQKADKVLYVNPNTGKKTWYSLPREVFLKAQAQADQAKAVWDTAAATYAQ
                     STLTAPFDGVVVDVAVIPGELVQVNQVVITIADLSDLQITTTDLSERDIARVKLGQSV
                     NVSIEALGQTISGKVIRISPVSETVGGDVVYPVTIQLDEQPDGLMWGMSAEVEISTK"
     gene            complement(3488..3898)
                     /locus_tag="PKI33_00570"
     CDS             complement(3488..3898)
                     /locus_tag="PKI33_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM35517.1"
                     /translation="MPLRLLMHPAHPARDSYRILIVDDAPTVRESLKWLLEDESGLTV
                     IGDAGNGDDAIQMAVNLKPDLVLLDIELPDMDGFAVAKQIKALLNPPRIILLSMHNDA
                     VSKKRGAEAGCDAFVGKEFGWGVLLPVLRTVLES"
     gene            complement(3920..5362)
                     /locus_tag="PKI33_00575"
     CDS             complement(3920..5362)
                     /locus_tag="PKI33_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35518.1"
                     /translation="MNTFLKSRIWTIFLLVSLLIVACGGSATEAPVATEAPATEAAPT
                     GPTYTIIGAVNRDATQAAQAPFVTVDAQKNLLVVWAENSSGGVRQIFASQLNGTAFQP
                     LGSSINIHTNVLADSPSITVAGENNAVPWVAWSEPSPGFDNVAQVFASRFSVETGLWQ
                     QAGTDRGGGEASLNISTERAGTHPFIFSGSTEIGNPPVPWVAWEEVSDVNPSSVQIFV
                     AKGVKDESAIGGFRWETVGQVKEDGEQTLNVNRFRISQHPTGVFAETSNSVPWVTWQE
                     QGRDEPSRIFTARGVADPNTPGGFKWINVPPCDPKDETKCALNINPLNDAKDASMASG
                     STVPGEGNVPWIAWPEIGQNGKWQIFVSRLDTSTRNSFLQVGGSLNIDPNHDAKLPTI
                     VFVGNVPYVAWLEDDGTGKFEVQMRHLASDPQTGTWALDTPAEGFNKDKTLSDFNLSV
                     VASTDTVFLAWGEGDPATSASQMVIGAFRP"
     gene            complement(5423..>7456)
                     /locus_tag="PKI33_00580"
     CDS             complement(5423..>7456)
                     /locus_tag="PKI33_00580"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNM35519.1"
                     /translation="DANTKLTEVQVGEEPRSVVITPNKAFAYVTNQVSGTVSVIDLNS
                     QTKVTDITVGAEPYGIALTPDGTRAYVANSASNTVSIIDTTTNTVVGTINIPGVQPRG
                     VAITNNNGGTGQQFVYVTQFLSQPTASGGPGLDQGSEGKVFVISTTDDAQIQGVITLG
                     AHDTGFTADRTAFGGTDKEPTFAYPNQMQSIILKNGRGYLPNVAASPEGPVKFNVDTQ
                     AFLSVFDVAAKSELAGGTINLHAAVKAQTFTPKLFLANPWAIAFKHNANEGYVVSAAS
                     DVLVKITIDDNGVPTVVSVPADGDTTRVLTIDVHKNPRGIVINNADTRAYVYNFISKD
                     VSVVDLTTSPEVELARVQSADLPAAGSDAAKFLAGNELFHSSRGEFDEGVAERMSNEG
                     WQACASCHPDGLSDGVVWAFNSGPRKSVPLNGTFSHENPTGNQRVLNYSAVFDELEDF
                     ELNIRNVSGGQGLIIQSGTTDPDPNVKAFDPPNANREQLHVGGFPAWDAIVAWTQFRV
                     PSPVSPYRGVDPSSELGQQIAQGRDLFTQANCQACHGGPKWSTPQVDFARVSPFPETI
                     TAGKPPEAPIGQLSRFLQIVGTFDPANPLEKNNNNQNALGQLGYNPPSLLSINAFPPY
                     LHNGSCLTLDCVLENQTHREAGGVTGVLDDPANREALVTFLKSIDITTEPITP"
BASE COUNT         1770 a         1854 c         1971 g         1861 t
ORIGIN      
        1 ccggatgaga tcgccagcca gatcagcgca ttggagccgg tgaaggctgc cacgccgcgt
       61 ttgtatgcca gcggatttct ttccacgggc gatgaatcga caggcgtgca gatcaacggc
      121 atcgacccgc agtctgtggc gaatgaccca tatcgcaacg ggatcatcag tggagaattc
      181 ctcaatgctg atgaccgtga aggtttattg atcggaagac cggcggcaga gaagttgcac
      241 ctgaaagtgg gcgacaaggt tagcctttcg gtcaacactg ccgatggcaa tgtgcaggag
      301 caggtcttta tcatccgcgg tatttacacc accgatacct atggctttga tagcggcacg
      361 gtcttcctgc cgttggcgaa ggcgcaggcg atcacacaga cggaagggca tgccagcacg
      421 atcttcgtgc ttctaaacga cacggcggat accgatcagg tcgtggctgc cttgaaatct
      481 tcgtcgagtt tggaagtgct tacctggcgg gatttgaaca aactctttgt agagttcgag
      541 accatggcgc aaagctatat ctcgttcttc tatctcatca tccttgccat taccgcctcg
      601 gtcattgtca acacgctcat catgtatgtt tacgaacgga cgcgcgaaat cggcatcctt
      661 tctgctattg gcatgcgcgg cagtcgtatt ttgggattgt tcctggcgga atcgttcatg
      721 ctggcagttg gcggcatcgt aatgggattg gcattgggcg tgtttgccgt ctatctcttc
      781 aatatcaatg gctttttcgt tggcaacatg gggctgagcg gtatgttggt ctcagatacc
      841 atccgcgccc agttgacagt ggataacacg attaatgtca ctgttatgac ctttattgcc
      901 accatccttt ccgggttgta tccggcggtg atggcatctc gattggagcc ggttcaggcg
      961 ttgcgtgcag aaaaataaac gcggaagaat ccgcgaggag aaatatcatg gaagttgtaa
     1021 agatcgaaaa cgtaactcgt acatatcaaa ttgggaaact ggaaacccat gccttgcgtg
     1081 gcgtcagcct ttccattaac agcggagaat tcacagccct ggttggtcct tcaggctcgg
     1141 gcaagaccac actcttgcaa atgatcggct gtctcgacca gcccaccagc gggcgcgtca
     1201 ccgtagatgg caaggaagtc acgtcgctga atcgtaatca gcgtgccgac atgcgcaaag
     1261 gtcatattgg gtttatcttc caattctttg ctttgatccc caccctcact gcctatgaaa
     1321 acgtggaaat gccgcttctg ctcaatgggc attctccaaa agaacgccgc gaccgtgtga
     1381 tggaattact caaagccgtg gatataaccg atcgtgccaa taatcgccct gatcaactct
     1441 ctggcggtca gcaacaacgc atcgccattg cgcgtgcttt ggcgcccaaa cctgcgctca
     1501 tccttgccga tgagccgact gctaatctcg ataccgagaa tggcaagcag gtgatggaga
     1561 tcatgaagaa gctgaatcaa gaaacagggg agacctttgt cttcgctaca catgatccgc
     1621 gtgtcattaa ttacgcttca cgtgtggtga ctttggaaga tggggtgatt gtcaaggatt
     1681 ctaagggata agccctttct tgacaaaacc tttgcaaaaa ctgtgcaata tgatatactt
     1741 tagaaacttc aaggattaca cagtttggag aaataaagaa tgaaacgagt tatttttgta
     1801 gttgttagtt tgctggctct tttgctgtcc gcgtgtggac aggctagttc aacagaagcc
     1861 gttcccgccg ttgtgatcga tgcgggcaat tcatcccagc ctgccgattc tcaaccttca
     1921 agcggcggtt cagtttctgc ttcggcgatt gttgttccca tgcaagatgc caacctttcc
     1981 tttaccaata ttggacgtgt gactcaggtc aatgtgcagg ttggcgataa ggttagcgca
     2041 ggtgatgtgt tggttcagtt ggataccacc attttggaag ccaaggttcg cgaggcagaa
     2101 gccaatttga aatacgcgca gatcagcctc gattatttga ttcgcaatgt gggctgtaag
     2161 atgaattgtg cgccttcaca ggaacacatt gaagtggcag agaatgacgt tgccaaggct
     2221 caagctttgg tggattcagc caaggctgtt ctcgccgctc aatcgaatct caccgcgccg
     2281 tttgctggta ccgttatctc ggtcgatatc gccccgtatg agacggtcgc tccgggacag
     2341 gttgtaatgg tgatcggtga cctctccaag tatcagatcg aaaccaccga cttgagcgaa
     2401 cgtgatgtga ctcaggtaaa agtgggtcag tctgcttcgg tatacatcga agcgttggac
     2461 gaagaattca ccggcaaagt tgtggacgtg gcacgcatct cttcagaggt tggcggcgat
     2521 gtggtctaca aagtgaccat tgaatttgat aaacagcctg caggcttatt gtggggcatg
     2581 agcgcggatg tcaacattga cgctggcgag tgaggttgcc atgaagcgat tgttaatcct
     2641 taccgttggc ttgttagtgc taacggcttg cgccagccca accccaacgc ctgttcctga
     2701 tgccgttgaa acaactgccc cggtcattga agtgtcttcg ccagatgtgg tgattgcttc
     2761 tgctgatgtc gaaccgactc acgtttctca ccttgggttt actatctcgg cacttgttaa
     2821 agaagttgcc gttggtgaag gggatgtggt taaggctggc gatacgttga tggtcttgaa
     2881 taccccagac cttcagtatg ccgtggtttc tgctgaggca gatttcaatg cacgcacgca
     2941 ggcagcacag cttcagaaag ccgataaggt tttgtatgtc aacccgaaca ccggcaagaa
     3001 aacttggtat agcttgcccc gtgaagtgtt cttgaaagcc caagcccaag ccgaccaagc
     3061 aaaagccgtt tgggacactg ccgctgctac atatgctcaa tccaccctaa cagctccctt
     3121 tgatggcgtg gttgtggatg ttgcggtcat cccgggtgag ttggtgcagg tgaatcaagt
     3181 cgttatcacc atagcagact tgagcgacct ccaaatcacc accacagact tgagcgaacg
     3241 cgatattgcg cgtgtaaaac tcgggcagag cgtgaacgtt tccatcgaag cgttgggaca
     3301 gaccatttca ggaaaagtca ttcgcatttc accggtttct gaaaccgtag gcggtgacgt
     3361 ggtctacccg gttaccatcc agttggatga acaacccgat ggcttgatgt ggggaatgag
     3421 tgctgaagta gaaatttcta cgaaataaaa aggaggtcac gttcaaaacg tgacctcctt
     3481 ttttgtatca actttccaga actgtcctta atacaggtaa caacacaccc caaccgaatt
     3541 cttttcctac aaaagcatca cagcctgcct ctgcacctcg tttcttagag accgcatcgt
     3601 tatgcatgga aagcagaatg atccgcggcg ggttgaggag ggctttgatc tgtttcgcaa
     3661 cggcaaagcc gtccatatct ggtaattcga tatcgagcag aaccagatca ggcttaaggt
     3721 tgaccgccat ttgaatggca tcgtcgccat tcccggcatc gccaataacg gtcaaccccg
     3781 attcgtcctc caacaaccac ttcaaacttt cacgtacggt aggcgcatca tctacgatca
     3841 gaattcggta actatctctt gcagggtggg cagggtgcat gagaagcctc aggggcatgg
     3901 agttctgcat atcagctcgt tacgggcgga atgcgccgat aaccatttgc gaggcagagg
     3961 ttgcagggtc accttcgccc caagcaagga agaccgtatc tgtagaagca accacagaga
     4021 gattgaaatc tgaaagggtt ttatctttat tgaagccttc ggctggggta tcgagcgccc
     4081 aagttccggt ttgtgggtct gaggcgaggt gtctcatctg cacttcaaat ttgcctgtgc
     4141 cgtcatcttc taaccaagcc acatatggaa catttccaac aaacacaatg gtcggtaact
     4201 tggcatcatg attcgggtca atgtttaaac tgccgccaac ctgaagaaaa ctattacgcg
     4261 tgctggtatc caaacgcgag acaaagatct gccatttgcc attctgacca atttcgggcc
     4321 atgcgatcca tggcacattg ccttcaccgg gaacggtgct gccagaagcc atggaagcat
     4381 ctttcgcgtc attcaacgga ttgatgttga gcgcgcattt ggtttcatct ttcgggtcac
     4441 aaggcggaac attgatccac ttgaaaccac cgggcgtgtt ggggtctgcc acaccacgtg
     4501 ccgtaaaaat gcggctgggt tcatcacgtc cctgttcttg ccaggttacc cacggtaccg
     4561 agttcgacgt ttcagcaaac acgcccgtag ggtgctgtga gatgcggaag cgattgacat
     4621 tcaaagtctg ttcgccatct tctttcactt gcccaacggt ctcccatcgg aagccgccaa
     4681 tcgcactttc atcctttacg cctttggcta caaaaatttg cacagaagat gggttcacat
     4741 ccgagacttc ttcccatgcc acccacggaa cgggcggatt cccgatctct gtggaccctg
     4801 agaaaataaa tggatgcgtg cctgcgcgtt ctgtcgagat gttgagactc gcctcgccac
     4861 cccctctgtc tgtgcctgct tgctgccata aacctgtctc tacactgaag cgactcgcaa
     4921 acacctgcgc cacattgtcg aaaccggggc tgggctcaga ccacgccacc caaggcacgg
     4981 cattgttctc acctgcaaca gtgatgctgg gcgaatctgc aagcacattg gtgtggatat
     5041 tgatggagga gcccaaaggt tggaaggcag ttccattcaa ttggctggca aagatctggc
     5101 gtactccgcc agaggaattc tcagcccaaa caaccagtag attcttttgc gcatccactg
     5161 ttacgaatgg agcttgagcc gcttgggttg catcccggtt aacagcacca ataatggtat
     5221 aggtgggtcc ggtaggggca gcttctgtag ctggggcttc ggtggcaaca ggcgcttctg
     5281 ttgcagaccc gccgcaggct acgattaata aacttacgag taagaagatc gtccatattc
     5341 ttgatttcaa gaatgtattc ataagggttc tccttcagcg agagagagtt gtctccctct
     5401 cgctgaaatg tgacgaacaa atttacgggg taattggttc tgtagtgata tcgatggatt
     5461 tgaggaaggt gaccaatgct tcacggtttg ctgggtcatc caatacgccg gtcacgccgc
     5521 ccgcttcgcg atgggtttga ttttccaaga cacaatccaa ggtcaggcag gagccgttat
     5581 ggagataagg cgggaaggca ttgatgctca acaaagaagg tgggttgtag cctaactgac
     5641 ccagcgcatt ctgattgttg ttgttcttct caagcgggtt ggcagggtca aaggtgccga
     5701 cgatctgcaa gaagcgtgag agttgaccaa ttggagcctc gggtggtttc ccggctgtga
     5761 tggtttctgg gaagggtgaa acgcgagcaa aatccacttg aggcgtggac catttgggtc
     5821 caccatggca agcctgacag ttcgcttgcg tgaagaggtc acgtccctgt gcgatctgtt
     5881 gaccaagctc actgcttgga tctacgccac gataagggga aacgggggaa ggtacacgga
     5941 attgtgtcca cgcaacgatc gcatcccaag cggggaagcc gccaacatgc aattgttcac
     6001 ggttggcatt gggtgggtca aaggctttta cattcgggtc tgggtcggtg gtaccgcttt
     6061 gaatgatcaa gccttgcccg ccagagacgt tacgaatgtt caattcaaag tcttccaact
     6121 catcgaacac tgcggagtaa ttcaacacac gctgattgcc agtggggttc tcatggctaa
     6181 aggttccatt caagggcacg ctcttgcgcg gaccggaatt gaacgcccac accacaccat
     6241 cgctcaagcc atcggggtga caacttgcac aagcttgcca accttcgttg gacattcttt
     6301 ccgccacgcc ttcgtcaaac tcaccgcgtg aagaatggaa caactcgttc ccagctagga
     6361 attttgcggc gtctgaacct gcagcgggca aatctgccga ctggacacga gccagctcaa
     6421 cttcgggtga ggtggtgagg tccaccacag agacatcttt ggaaatgaag ttgtaaacgt
     6481 atgcgcgagt atccgcattg ttgatgacga ttccgcgcgg atttttgtgg acatcaatcg
     6541 tcaagacgcg cgttgtatct ccatccgccg gcacagaaac taccgtaggc acaccgttat
     6601 cgtcgatagt gatcttgact aatacatcac tcgctgctga aacgacatag ccttcattgg
     6661 cattgtgctt gaaggcaatt gcccatgggt tggcaaggaa taacttgggc gtaaaggttt
     6721 gtgctttaac agccgcatgc aaattgatcg tgccgcctgc caattcagat tttgcagcta
     6781 catcgaatac cgaaaggaag gcttgtgtat ctacattgaa cttcacaggt ccttcaggag
     6841 aagccgctac gttgggtaaa tagccgcgtc catttttcaa gatgatggat tgcatctgat
     6901 tcggatacgc aaaggttggt tctttgtctg tgccgccgaa cgcggttcga tctgcggtga
     6961 agcctgtatc atgtgctccg agcgtgatca cgccttgaat ttgtgcgtcg tctgtcgtgg
     7021 aaataacgaa gactttgcct tcactgccct gatccaaccc cggtccacca gaagcggtgg
     7081 gttgggagag gaactgggtt acataaacga attgctgccc ggttccgccg ttgttattgg
     7141 tgattgccac accgcgtggt tgcacaccgg gaatattgat cgtaccgaca accgtattgg
     7201 tcgttgtatc aatgatcgaa actgtattcg aggcagaatt tgcaacatag gcacgggttc
     7261 catcgggggt gagcgcgatc ccgtacggct cagctccaac ggtgatatca gtgaccttgg
     7321 tctgcgaatt caagtcaatc acagagaccg taccgctgac ttgattggtt acataagcaa
     7381 aagctttgtt cggtgtgatc accacagaac gcggttcttc gcctacttgt acttcagtca
     7441 acttggtgtt ggcatc
//
LOCUS       DAOREF010000014         7322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5107_length_7322_cov_4.832943, whole genome shotgun sequence.
ACCESSION   DAOREF010000014 DAOREF010000000
VERSION     DAOREF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5107_length_7322_cov_4.832943"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PKI33_00585"
     CDS             <1..813
                     /locus_tag="PKI33_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNM35520.1"
                     /translation="RGRKETSVTLFYLWLTGKVMITNRKGFDRIYDLRERVVPKEFDY
                     AASVKETEDFFTKKHIAFYGVARENSLKTDLSYYIQRKVDAQESKKHIEQLIEKREIA
                     RVRVEGLSDTFLVLSSDQAHLSALEAGHIPKVWKHKGPSTSEEVTFLAPLEIVSARGR
                     AKKVFDFDYVWEVYKPVHQRKWGYYTLPILYGDNLVARMDPKHDRTTNTLQILGFWLE
                     NDAPKDSNFVNALANGMKRFADMIGAKKIDLAGVKPPKLKTELKVLLKRIDY"
     gene            846..1298
                     /locus_tag="PKI33_00590"
     CDS             846..1298
                     /locus_tag="PKI33_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM35521.1"
                     /translation="MTYSLRPANLSDAPAVAACVNDAFAHYVERIGMKPAPMEMDYEH
                     EIRTHQVFVVEDAGEVVGSLVLGITEEGFLLDVIAVAPKYWGRGVGRVLLEQAEAEAK
                     KQGFDSIYLFTHEKMIENQALYKKIGYVEYDRRLEMGRRRVYMRKQLV"
     gene            1517..6502
                     /locus_tag="PKI33_00595"
     CDS             1517..6502
                     /locus_tag="PKI33_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM35522.1"
                     /translation="MVSGESTGAKKQVLKEGQSSHAHYLLLSLSRAAQAIQQAHTAED
                     FYIAVGREINQLGGEVTLLLLDEAGTKLSIAYTSYSPKLISRAEKLTGLSLKNYKLPI
                     PPESAYEVALQGESAIFIDASDEIISNILPDALAFLSTQIISLFNLRQGILAPLRVDK
                     KVLGLLKVNGNFLNLDDLPAMESFAGHIAAGLHNVHLMQKLQEELTAHKQTEQALQHN
                     RNLLLALSQAAHAIQLVRMPDDIYKVVGEQIRALGFEATILKFGSDMNSLHYRYTTIP
                     EKVLHTAEKLAGIKAEDFGWAISQDSVYARVLINRKAEYISNGKALFIEALPKFLLPL
                     AEKLVDLVSSHHGILAPLHVDDEAFGIMLVFGSDQISEEDVPAVESFSAQVSVSLRNA
                     YLTQRLQRELHERKQAEAAAQQAEKRFKALIENAPDGITLVGIDGKATYASPSAKEMF
                     GYVGDEYINENPMDSIHPDDIGAVLATMNNLVGDSSSPRTVQYRYRHQDGSYHWVESI
                     FSNQFAEPAVEAIVVNFRDITERKEMERALYESERYYRALIENTADGILVIDGSGVVT
                     YESPSVARLLGYGQDKLIGTSSFNLIHPDDLSEILKTFVDGLNSPGFVHRGEYRLLNA
                     ENNWRYFEIVTHYLMDDPAIMGVIINGRDITERKQSESALKESERKFHGVIDKSADGI
                     ALSDEEGRILEFNEAFEQLTGKKRENVLGKFLWELAYELMPDSTKADDENFTLRNRIQ
                     QALKIGATSYFQFTQEVPFQRADGSVLIIQQRIFTIRTDKGCRLGSISRDITEIKKVE
                     EYLTQQNKQLNSLYQITSALSQINAVEDIYAIALDSLCSNLDIKRVSILLFDRDGVMR
                     FKAWRGLSDRYRRAAEGHSPWKPDAVDPQPVLVEDAFTDAELASLYELFKSEGIGALG
                     FIPLVHQNKLLGKFMIYFDAPHHFLETEVRLAQAIASHVAFAIHKSALNEALRTNEEQ
                     YRILYEDNPSIYFTTDDMGTILSVNKTGVEQLGYAIDELIGSPLLNIFYPDDREHVQQ
                     KMLVCINDPELTVQVEARKQRKDKSILWVREFARAIRNTDGQLVILIVCNNVTEEVKA
                     EEALVQSEAELRALFSSMKDSVIVIDRKGIYQSIAPTNPETLYIPPAEVIGKGLSAFF
                     PEEQVQEFLKAIEYVLATGKTVIIEYQIILADASPWFESRVSPLGPDTTIWVARDVSE
                     RKKIQSALIQSEQAYRSLFENMPIGLYRSSIHGELLDANPSLLRMFGYEDLETMKGKK
                     AWEFYNDPALDDEFRQQISENGFLNRYESEFIRQDGTRFWAEDYVRAVYDGKGTPLYY
                     EGSLIDVTDRKAAEKRLKESEKQYRLLAERIADVVWVLDLATLEFKYISSSVQNLLGH
                     SAEELLNKHVSMVLTPSAMMAIESITPERIKRFLAGDKTSVTELYQLDHVHKDGSIIV
                     VEVSSTFVMNENATIEAIGVTRNITERKKAEDDLRLANSSLQIAHKELQQLFEYEQIL
                     ARTDGLTRLYNRRYFFELAAREFNSAIRYQRPLTLIIFDVDGFKQANDTFGHDFGDRT
                     LVEVSKTANLQVREVDILARYGGDEFTILLPETNVEQAFLIAERIRKAVADVYIDMGG
                     FSTFVTISMGIAELSFEPQDQSIEDMIRRADQALYQAKQQGRNKVVIYSDK"
     gene            6565..7218
                     /locus_tag="PKI33_00600"
     CDS             6565..7218
                     /locus_tag="PKI33_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35523.1"
                     /translation="MIKPVRWNTFIRDFIRIQIGFLLYGLAITLMIRGNLGTSAWAVL
                     EVALADFLHITVGTMTVIMGFIVLILSLLMREQIGWGTLGNILSIGPWVDLCMALVPT
                     VNNNLAIQILMLLSAIIIMGLASAIYIGVDAGAGPRDSLMLAIRRKTGVSIRVSRAII
                     EVTVVVLGWLLGGPAGIGTVVHAVLIGPSVQWGFKVFKVQAHTEREEVQAGIEGGAE"
BASE COUNT         2090 a         1422 c         1753 g         2057 t
ORIGIN      
        1 cgtgggcgca aggaaacctc ggtcacattg ttctatctct ggctgacggg gaaagtgatg
       61 atcaccaacc gcaaaggttt tgaccgtatc tacgacttgc gcgaacgtgt agtgccaaaa
      121 gaatttgatt atgcggcttc ggtgaaagaa actgaagatt tttttacaaa aaagcatatc
      181 gccttttatg gtgttgcgcg tgaaaactca ttgaagacag acttaagtta ttacatccaa
      241 cgtaaagtgg atgcgcagga aagtaaaaaa catattgaac agcttattga aaaacgcgag
      301 atcgcgcgag ttcgtgtgga aggcttgagc gacaccttcc ttgtcttaag cagcgaccaa
      361 gcccacttgt ctgctcttga agcggggcac atcccaaagg tatggaagca taaaggtcca
      421 agcacgtctg aagaagtcac tttccttgct ccgttggaaa ttgtcagcgc tcgcgggcgt
      481 gccaagaaag tttttgattt tgattacgtg tgggaagtat acaaaccagt tcatcagcgt
      541 aagtggggct attacacact gccgatcttg tatggagata acctggttgc gcgtatggac
      601 ccaaaacatg accgcaccac gaatacgctg caaattctag gtttctggtt ggaaaatgat
      661 gcgccaaaag attccaattt cgtgaatgca cttgccaatg gcatgaaacg cttcgcagat
      721 atgatcggtg caaagaagat cgatctggct ggagtaaaac ctccgaaact gaaaacagaa
      781 ttgaaagtgc ttctaaaaag aattgactat tgattttgtg cgataaattt tcaagggact
      841 gatatatgac ttactcttta cgccctgcca atctttctga tgctcctgct gttgcggctt
      901 gtgtcaatga tgcctttgct cactatgtgg agcggatcgg aatgaaaccc gcccccatgg
      961 aaatggatta cgagcatgag attcgtacgc atcaagtatt tgtggtggaa gatgctggag
     1021 aggtcgttgg ctcgctcgtt cttggcataa ctgaagaagg ctttttattg gatgtgatcg
     1081 cagttgcccc caaatattgg ggcaggggtg tgggacgcgt cttgcttgaa caggcagaag
     1141 cagaagcaaa aaaacagggg tttgatagta tctatctctt tactcatgag aagatgatcg
     1201 agaaccaagc actgtataaa aagatcgggt atgttgagta tgaccgccgc cttgaaatgg
     1261 gacggagacg tgtatatatg cggaaacaac ttgtttagac taaaccaatt tggtctaatt
     1321 gcttgactac aagagcaaga caatatatat taatactata ttgggaattt tcattcattg
     1381 ctattatatt tattcttcac catcctttaa ctctcccaat gtgaaaattt agatcatcga
     1441 attcgtaaaa cctgagcaag gttctttact gtctttgtat gtagaaaagg caaaaagaat
     1501 tcgagaggat gccaagatgg tttcaggtga atccactggg gcaaagaaac aggtcttgaa
     1561 agaggggcaa agcagccatg cgcattatct tttgctgtca ttaagccggg ctgcgcaagc
     1621 gatccaacag gcacatacag cagaagattt ttatattgcg gtaggcagag agatcaatca
     1681 actaggtggg gaagtcactt tgttgctgct tgatgaagca gggacgaagc tttctattgc
     1741 ttatacatcc tattccccaa aattaatcag ccgggcagaa aagctaaccg ggctatccct
     1801 aaaaaattat aaacttccaa ttcctcctga aagcgcatat gaagttgctt tgcagggaga
     1861 atctgcgatt tttatcgatg caagtgatga gattatttca aatattctgc cagatgcgct
     1921 tgcctttttg tctactcaaa ttatctctct ttttaatttg cgtcagggta ttcttgcgcc
     1981 tctacgtgta gataaaaaag ttttgggact gctcaaggtc aacggaaatt ttctcaatct
     2041 ggatgatctc cctgcgatgg agtcattcgc agggcacatt gcggctggac ttcataacgt
     2101 ccatttgatg caaaaactac aagaggaatt aacggctcac aaacagacgg agcaggctct
     2161 tcagcataac cgtaaccttt tacttgcctt gagccaggca gcccatgcca ttcaactggt
     2221 gcgcatgcca gatgatatct ataaagtggt tggagagcag atccgcgcgt tgggctttga
     2281 agcaactatc cttaagtttg gaagcgatat gaatagtctt cattatcgct atacaaccat
     2341 cccagaaaaa gtacttcata ctgctgaaaa attggcaggt attaaagcag aagattttgg
     2401 ctgggctatt tcgcaagata gtgtttatgc tcgtgttttg atcaatagaa aagcggaata
     2461 catttcaaac ggaaaagctc tttttattga ggcgcttccc aaatttttac ttccgctagc
     2521 tgaaaagcta gttgaccttg taagttcaca tcatggcatt ctggctcctt tgcatgttga
     2581 tgatgaggcc tttggcatta tgctcgtgtt tggaagtgat cagataagcg aagaggatgt
     2641 tccagctgtc gagtcttttt ctgcccaagt ttcggtcagt ttgcgaaatg cctacttaac
     2701 tcaaaggttg caaagagaat tgcatgaacg aaaacaggca gaagctgccg ctcaacaagc
     2761 agagaagcgc ttcaaagctt tgattgaaaa tgcgccggac ggtattaccc ttgtaggaat
     2821 agatggtaag gctacgtatg ccagcccatc tgccaaagag atgtttggct atgtcgggga
     2881 tgaatatatc aatgaaaacc ctatggattc tatccaccca gatgatatag gcgccgttct
     2941 tgcaacgatg aacaatctgg taggagattc ctcttctccc cgtacggttc agtatcgtta
     3001 ccgacaccaa gatggttctt atcattgggt ggaaagtata tttagcaatc agtttgcaga
     3061 acctgcagta gaggcaatag ttgttaactt cagagatatt accgaacgta aggagatgga
     3121 aagagctcta tatgagagtg aacgctacta tcgcgctctg atagagaata ctgcggatgg
     3181 gattctagtg attgatggga gcggggttgt gacgtatgaa agcccatccg ttgcacgttt
     3241 gttggggtat gggcaagata aattgatcgg tacatcctca tttaacttga tccatcctga
     3301 tgatttatct gagattctta agacgttcgt cgatggctta aattctccag gatttgtaca
     3361 tcgtggtgaa tatcgcttat tgaatgcaga aaacaattgg cgctattttg aaattgtcac
     3421 tcactacttg atggatgacc ctgcgattat gggcgtcatt atcaatggac gggatattac
     3481 agagcggaaa cagtctgaga gtgccttgaa agaaagtgaa agaaaatttc atggcgtaat
     3541 tgataaatct gcagatggca ttgcactatc ggatgaagag ggacgcattc ttgaattcaa
     3601 tgaagcattt gaacaactta cagggaaaaa gcgagagaat gtcttaggta aattcctgtg
     3661 ggagcttgct tatgaactca tgccagacag tacaaaagca gatgatgaaa actttactct
     3721 tcgaaatcgg attcaacaag ctctcaaaat tggcgctact tcttatttcc aatttacgca
     3781 ggaagtccca tttcagcggg cggacggtag tgtattaatc atccaacaaa gaatatttac
     3841 gatccgtaca gataaggggt gcaggcttgg ttcgatctcg cgggatatta ccgagatcaa
     3901 aaaagtagaa gaatatctca cgcagcaaaa caaacaactg aatagtttgt atcaaattac
     3961 ttctgcttta agtcaaatta acgcagttga agatatctat gccattgccc tcgatagttt
     4021 atgtagtaat ttggatatta agcgggtatc cattctatta tttgatagag atggggtgat
     4081 gcgttttaaa gcttggcgcg gattgtcaga tcgatatcgc cgagcagcag aagggcactc
     4141 tccttggaag ccagatgcgg tggatccgca accggttttg gtagaagatg cattcactga
     4201 tgctgaactc gcttctttgt atgagctctt taagagcgag gggattggcg ccctcggttt
     4261 tattccgctt gttcatcaga acaaactgct tggcaagttt atgatctatt ttgatgcccc
     4321 acatcatttt cttgaaacag aggttcgctt agctcaggca atcgcttcgc atgtagcgtt
     4381 tgctattcat aagagcgcat taaatgaggc attacgtacg aacgaagagc aatatcgtat
     4441 tctgtatgaa gataacccat ccatatattt cactacagat gatatgggca ctatcctttc
     4501 tgtcaataaa actggtgtgg aacaattagg gtatgccatt gatgaactga tcggcagccc
     4561 actattgaac atcttttacc cagatgaccg cgaacatgtt cagcaaaaga tgttggtgtg
     4621 tatcaacgac cctgaactta ctgttcaggt tgaggcgcga aaacaaagga aagataaaag
     4681 cattctatgg gtgagagagt ttgcgcgtgc tatcaggaat acagatggac aattggtgat
     4741 tctgatcgtt tgtaacaatg ttacagaaga agtcaaagct gaagaagccc ttgttcagtc
     4801 tgaagctgag ctacgggcat tattctcttc aatgaaagat tctgttattg tgattgaccg
     4861 aaaagggatc tatcaaagta tagctcctac aaacccggaa acactttata tccctcctgc
     4921 cgaggtgatc gggaagggac tatccgcgtt cttccctgaa gagcaagttc aggaatttct
     4981 caaggcaatt gaatatgtgc tagcaaccgg taagacagtc attatcgaat atcagatcat
     5041 tttggcagat gcttctccat ggttcgagtc gagagtctcg ccgttgggac ccgatacaac
     5101 catctgggtg gcaagagatg tttctgaacg taagaagatc caatcagcat tgatccaatc
     5161 tgaacaagcc tatcgttctt tgtttgagaa catgccaatc ggactatatc gttcttctat
     5221 ccatggcgag ttgctggatg ccaacccatc tttgttgcga atgtttggat atgaagattt
     5281 agaaacaatg aaggggaaga aggcatggga gttttataat gaccctgctt tagatgacga
     5341 gttccgacag cagatttctg aaaatggttt cttgaatcga tatgagtctg aatttatccg
     5401 tcaagatggg accagattct gggcagaaga ttatgtccgt gctgtgtatg atgggaaagg
     5461 tacgccgctt tattacgaag gcagtctgat cgatgtgacg gatcgaaaag cagcagaaaa
     5521 acgacttaag gaaagcgaga aacaataccg cctattggct gaacgaattg ccgatgtggt
     5581 ttgggttttg gatctggcaa ctcttgaatt caaatatatt agctcgtctg tccaaaacct
     5641 tttagggcat tctgctgagg aattgctgaa taaacatgtc agcatggtgt taactccttc
     5701 tgctatgatg gctattgaga gcatcacccc tgaacgcata aagcgcttcc tcgctggaga
     5761 taaaacatct gtaacagaac tttatcagtt ggaccatgtt cataaagatg gttcaattat
     5821 tgtggttgaa gtttcaagca catttgttat gaatgaaaac gctacaatag aagcgattgg
     5881 tgtgactcgt aatattaccg agcgaaagaa agcagaagat gacctgcgcc ttgccaatag
     5941 ttctcttcaa attgcccata aggaacttca gcaattgttc gagtatgaac agattcttgc
     6001 tcgaaccgac ggtttgacgc gtttatacaa ccgccgctat ttctttgagc ttgctgcacg
     6061 cgagtttaac tccgccattc gctatcagag acctttgaca ttgattattt ttgacgtcga
     6121 cggtttcaaa caggcaaacg atacattcgg acatgatttt ggggatcgca cattggttga
     6181 ggtttccaaa acagcaaatt tacaagtacg tgaagtggat attttggcac gctacggtgg
     6241 tgatgaattt acgattcttt tgccagagac aaatgtagag caggcttttc tcattgcaga
     6301 acgtattcgt aaagcagttg cagatgtcta tattgacatg ggtggtttct ccacgtttgt
     6361 tactatcagc atgggaatcg cagaattatc ttttgaacct caagatcagt cgatcgaaga
     6421 tatgatccgt cgagcagacc aggctttgta tcaggcaaaa caacagggta ggaataaagt
     6481 cgtaatttac tcagataaat aatcgcatca caaaaaatca aggtttatag cggcatggta
     6541 tcatgatgct ttcaaaggag catcatgatt aaaccagttc gttggaatac atttatccgc
     6601 gattttattc gcattcagat cggctttctt ttgtatggct tggcgatcac cttaatgatt
     6661 cgcggaaacc tcggaacaag tgcttgggca gtattggaag tggcattggc agacttttta
     6721 cacattacgg tgggtaccat gactgttatt atgggcttca tcgttttgat actctccttg
     6781 ttaatgcggg aacagatagg gtggggaacg ttaggaaata ttctttccat tggtccctgg
     6841 gtggacttat gcatggcgtt ggttcctact gtgaataaca atcttgctat acagatactc
     6901 atgttgttaa gcgccatcat cattatggga cttgccagcg ccatctatat tggagttgac
     6961 gcaggcgctg gaccaagaga cagcttgatg cttgcgattc ggcgtaaaac gggtgtgagc
     7021 attcgtgtat cgcgagcgat cattgaagtg acagtggtgg tacttggctg gctattgggt
     7081 ggacctgccg gaattggcac ggttgtccat gctgttttga ttggtccctc cgttcaatgg
     7141 ggcttcaaag tattcaaggt gcaggcgcat accgagcgag aagaagtaca agcaggcatt
     7201 gaaggtggcg cagaataaat gcggaacgat taaggagtga actatgaatg actttactca
     7261 aaatgcacca gttgtagaaa agaaacacag caatggcgcc ttggcattat ttctcattgt
     7321 gc
//
LOCUS       DAOREF010000015         7053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5417_length_7053_cov_3.993427, whole genome shotgun sequence.
ACCESSION   DAOREF010000015 DAOREF010000000
VERSION     DAOREF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5417_length_7053_cov_3.993427"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKI33_00605"
     CDS             complement(<1..838)
                     /locus_tag="PKI33_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNM35524.1"
                     /translation="MNIMISGGTGLLGTALTKALLADGHKVFILSRTDKKRSDVQIIK
                     WDARTPDGWGHLVNEMDVVIHLAGRSTSAWPWSAANKKSFEDSRILPGLALAQAIQEA
                     TRRPSLFVQVSGINHYGLQGGLADETTPPGDDFLAQLTVKWEAATQSVEGLGVRRLVL
                     RTPPVLSRDNVIMKLIALPVQLFVGGPIGSGKQPFPWIHIKDWVSAVRYLMTDESARG
                     VYNMIAPSQTSLADFTKLLASVLHRPYWFPVPAFLMRNVLGEMSVLILEGRFAQPKRL
                     LES"
     gene            923..1426
                     /locus_tag="PKI33_00610"
     CDS             923..1426
                     /locus_tag="PKI33_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNM35525.1"
                     /translation="MNEPVLVLNANFEPIHVCSTRRAVTLILAGKAGMIANGRGHIRT
                     ISQLIPRPSVIRLESQIHRPRPRVKLTRREIFRRDNYTCQYCGKRDTNLTIDHVIPRH
                     MGGKQVWTNLVTACSPCNHRKGGRKVDEAHMHLLHIPKEPPADASYIFGKHLAENQEW
                     TPYITGW"
     gene            complement(1460..2215)
                     /locus_tag="PKI33_00615"
     CDS             complement(1460..2215)
                     /locus_tag="PKI33_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM35526.1"
                     /translation="MAKVIILGASNAIPTSESENTHMVIVGNDRTVLIDSVSNPILRL
                     EQAGLDFNNLTDIIITHFHPDHASGIPLLLMDMWLMGRQKPLNIYGLHYTLDRVEGLM
                     GFYNWSEWPNFFPVVFYRLPVREMTPVLDCPDFIIHASPVHHMIPNIGLRVNFQQSQK
                     VMAYSCDTEPCDEVVRLSEGADVLIHEATGESPGHSSATQAGEIASRAEVNKLYLIHY
                     PTGRFKKGDIAAEASQTYQGEIQIAKDFMVLDF"
     gene            complement(2317..3750)
                     /locus_tag="PKI33_00620"
     CDS             complement(2317..3750)
                     /locus_tag="PKI33_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNM35527.1"
                     /translation="MFNKLRPVWLLAQRELRDQFRDWRVLIPMAILTFCFPFLMNEFA
                     KQTVDFLNQYNANLILDRLVPFSIMIIGFFPITVSLVVALESFVGEKERGTIEPLLTA
                     PFDDWQLYFGKLLVGAATPLIASYVSIGLYMLLIIRQDLTMPSASVMVQLLLLTGAHA
                     VLMVSAAIVISVQSTSVKAANLLASFIVIPVAILMQGEAVMLFWGNETVLWLGVAGVL
                     IISLLLIRVGIAHFEREYLLGREIDNLNFKWMLRTFSKNFTGGAASVGDWYRRVVASS
                     FRRALPAMGWMLLVAVVSVWMSYNWVTTNVSQAITSASPDTLAKLEKGIENLPDLSSL
                     GGQLSAPFLFFNNTRSMTVIFLAGIFSFSVLGVLLYMVNIALIGGVYAVLELLGIAPA
                     PIFLAGILPHGIFELPALLIGSAVVLYIGAALVTPQNGKSMGEVMIDLMADWAKIFVG
                     VVVPLLAIAALIEAYITPSILLRVVGN"
     gene            complement(3740..4675)
                     /locus_tag="PKI33_00625"
     CDS             complement(3740..4675)
                     /locus_tag="PKI33_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM35528.1"
                     /translation="MIETNDLSKQFHDFMAVDGVNLSIGAGQILALLGQNGAGKTTTV
                     RMLTALLQPSRGSARVAGYDVTKNGHDVRASVGVLTEHHGLYMRMTGSEYMDFFGQVY
                     NLDPSLRKSRTENLLEYFGLAEASKRRIGEYSKGMRQKLALARAMMHEPSVLLLDEPT
                     SAMDPESARLVRDEIARLKSTQRTIMLCSHNLTEVELLADKIAIIYRGKILIQGSLDE
                     LKSGVLGAPEYVIKFASAWDASRLEMPKGMELLEKTATSLKVRVEAPAESNPELIQRL
                     TSLSAPVVAFQEETRTLEQVYLKVMATAKGKEYVQ"
     gene            complement(4753..5553)
                     /gene="lgt"
                     /locus_tag="PKI33_00630"
     CDS             complement(4753..5553)
                     /gene="lgt"
                     /locus_tag="PKI33_00630"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNM35529.1"
                     /translation="MTDGLNLGFLLIRWNGLLIALGIAVGALVAAIEAKRRFQDAELI
                     YYLFVPVTVWSWIGARLWHVFTPPLSSVQLGLTTSHYLSHPLDILSVWTGGFGIPGAI
                     LGGAFALWLASRKYEFSFWEVADALAPGMLLAQAIGRIGNYFNQELYGLPTNLPWAIF
                     IQPDHRLLGYEQIEFYHPLFAYEAILVFLGFVLLFWLSRSKFADTFFAGDIYLGSLAF
                     YSLVRFLLENLRLDVALVNGVNANQLFFAILFIVSISILGHRRLAPVL"
     gene            complement(5550..6179)
                     /locus_tag="PKI33_00635"
     CDS             complement(5550..6179)
                     /locus_tag="PKI33_00635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNM35530.1"
                     /translation="MNTKPTFSDRARIIFKGILDPIGNFLNGIGLTPNTITLLGLAGT
                     TVGAYVISQGHMTTGAFILLASVLVDAFDGTMARLRGEPSDFGGFVDSVSDRYAELVT
                     FGGLVYYFLTKADYPGVMISFFAAAGSVLVSYVKARAEGLGFTAKVGILTRLERYIVL
                     IPLLVFNQPFAAVIIIAVLANITAFQRILNVRAQGRERIKKSKESKEAK"
     gene            complement(6192..>7053)
                     /locus_tag="PKI33_00640"
     CDS             complement(6192..>7053)
                     /locus_tag="PKI33_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HNM35531.1"
                     /translation="KMVELIRFNGLPHLYGKVETNLERILGVLRWVVVEMEHRYRLLE
                     SMHARDLNSYNKKVSRSADGEQLPRIVVIIDELADLMMSAPDQTEHNLVRLAQMARAT
                     GIHLVVATQRPSTDVVTGLIKANFPARLAFAVASGTDSRVILDYTGAESLLGRGDMLF
                     LNPEVGTPVRAQGVLITDTEIERIIAYWQKNWDTDAVEVPPWETLLKEPEEDEDDGLV
                     EKAISIVRQQQRASASMLQRRLRIGYPRAARLLDQLETMGVVGPSQGGGKEREVLISD
                     EDEIGNQEER"
BASE COUNT         2038 a         1751 c         1622 g         1642 t
ORIGIN      
        1 cagactcaag tagccgttta ggctgggcaa accttccttc aaggatcaag acgctcattt
       61 cacccaaaac gttccgcatt aaaaaggctg ggacaggaaa ccaatatggg cgatgcaata
      121 cactggctaa aagcttggtg aaatcagcca atgaagtctg cgaaggcgca atcatattat
      181 agacgccacg tgcgctttca tccgtcatca aatagcggac agcactcacc caatctttga
      241 tatgaatcca agggaagggt tgtttgccag aaccaatggg tccgccaacg aataattgca
      301 ctggcaaagc aatcaatttc attattacat tatctcggga aagtacaggt ggtgtacgca
      361 aaaccaacct gcgaacgcct aatccttcaa cagattgagt cgcagcttcc catttaacgg
      421 taagctgggc aaggaagtca tcgccgggtg gagtggtctc atctgcaaga ccgccttgca
      481 agccataatg attgatgcca gagacttgca cgaaaaggct cgggcggcga gtcgcttctt
      541 ggatggcttg agcaagcgca agtccgggaa ggatgcgaga gtcctcaaag gatttcttat
      601 ttgcagcaga ccacggccaa gcggatgtac tgcgtccggc aagatgaatg acgacatcca
      661 tttcgttgac gagatgcccc cacccatcgg gggtacgggc atcccatttt atgatttgaa
      721 cgtcagaacg ttttttgtcg gtacgcgaaa ggatgaaaac tttatgacca tccgcaagaa
      781 gggcttttgt cagggcggtg ccaagcaagc ctgtgccgcc agagatcatg atattcattt
      841 ttgctcctgc aatgatatgt caatgggtgt ttaaactgat ttaacattct agatgtataa
      901 tcacgcgagt taccggaacc ttatgaacga acccgtactg gtgctcaacg caaattttga
      961 gccgatccac gtctgttcga cacgacgggc agttactttg atccttgcgg gaaaagccgg
     1021 tatgatcgca aacggacgcg gacacatccg caccatatca cagctcatcc ctcgtccttc
     1081 ggttattcgg ctggaatcgc aaatccatcg accacgccca cgcgtcaaat taacgcgccg
     1141 cgagatcttt aggcgcgata attatacctg ccagtattgc ggcaaacgcg acacgaactt
     1201 gacgatcgat catgtgattc cgcgccatat gggtggtaaa caagtttgga cgaatttggt
     1261 aacggcttgt tcgccttgta atcatcgcaa aggcgggcgc aaagtggatg aagcacacat
     1321 gcatttgctc catatcccca aagaaccacc tgccgacgct tcttatattt ttgggaaaca
     1381 cttggcagaa aaccaagaat ggacacctta tattaccggt tggtaaatga aaatcccctc
     1441 atcacgaagg gattttttat tagaagtcta gcaccataaa gtcttttgct atctggatct
     1501 cgccctgata ggtctgagat gcttcggcgg caatatcgcc ttttttgaat ctgccggtgg
     1561 gatagtgaat aagatacagt ttatttactt ctgcccgcga agcgatttct ccggcttgag
     1621 tggcagagga gtgaccgggc gattcgcccg tcgcttcgtg gataagcacg tctgcgcctt
     1681 cgcttaagcg aacaacttcg tcacaaggtt cagtgtcaca tgaatatgcc atcacttttt
     1741 ggctttgttg gaaattgacg cgtaaaccaa tgttgggaat catatggtgg actggagagg
     1801 catgaataat aaagtcggga caatccaaca cgggggtcat ctcgcggacg ggtaaacgat
     1861 agaaaacgac cgggaaaaag tttggccact cagaccagtt ataaaagccc atcaagcctt
     1921 caactcgatc aagagtgtag tgcagaccat agatattgag cggtttttgc ctgcccatca
     1981 gccacatatc cataagcaaa agcggaatac cagaggcatg gtcgggatga aaatgggtga
     2041 tgataatgtc ggtaaggtta ttaaagtcga gcccggcttg ctcaagccgt agaatgggat
     2101 tgctcactga atcgatcaga acggtacggt catttcccac aatgaccatg tgtgtgtttt
     2161 cgctttcgct tgtgggaatt gcgtttgaag ccccaaggat gatgactttt gccataatca
     2221 aatcttaact caaatcccct taattgttaa ttgtattttc atatgagcca taagaaaagc
     2281 gagctgcctt gtataaaaca gctcgctttt cttgaattaa tttccgacaa ctcttaatag
     2341 aatactaggg gtgatatatg cctcaattaa ggctgcgatt gcaagcaatg gcaccaccac
     2401 accaacaaat atctttgccc aatctgccat gagatcgatc ataacctccc ccatcgactt
     2461 gccattttgc ggtgtgacca aggcggcacc aatgtacaac actacggcac taccaattag
     2521 caaagccggg agttcaaaaa taccatgcgg caaaattcca gccagaaaga tcggagcagg
     2581 cgcaatgcct aaaagttcca aaacggcata tacgccgcca atcaaagcaa tgttgaccat
     2641 atacagcaga acgcccaaga cactaaacga aaatatccca gcgagaaata taacagtcat
     2701 ggaacgggtg ttgttgaaaa ataagaaggg cgcgcttaat tgtccaccca ggctggaaag
     2761 gtctggtagg ttttcaatac ccttttcaag ctttgccaac gtatcgggcg aggcgctggt
     2821 aatcgcttga gatacattgg ttgtaaccca gttgtagctc atccatacac tgacaaccgc
     2881 caccaaaagc atccaaccca tggcgggcaa tgctcgccga aaagaagaag caacaacgcg
     2941 gcgataccaa tctccgacag atgctgcgcc accggtaaag tttttcgaga aagttcgtaa
     3001 catccacttg aagttgagat tatcaatctc tcgtccaagt aaatattcac gctcaaagtg
     3061 cgcaatccct acccgaatca atagcaaaga gataatcaac acaccggcaa cgcccagcca
     3121 tagtacagtc tcattgcccc aaaacaacat cacagcttcg ccttgcatca gaatcgccac
     3181 tggaatcaca atgaacgatg ctaacaaatt tgccgctttc actgatgtgg attgcaccga
     3241 aataacaatc gcggcactaa ccatcaagac cgcatgtgcc ccagtaagca aaagcaattg
     3301 caccatcaca gaggcgctag gcatggttaa gtcttgacga atgatcaaca acatatacaa
     3361 gccaatggaa acatagctgg cgatcaacgg cgtagcagcc ccaaccagca acttaccaaa
     3421 atatagttgc caatcatcaa atggcgcagt caacaaaggc tcgatcgtgc cacgttcttt
     3481 ctcgcccaca aaagactcta atgccaccac gagtgaaaca gtaataggaa agaatcctat
     3541 gatcatgata gagaacggaa ccagacgatc taatatcagg ttggcgttgt attggttcaa
     3601 gaaatccacg gtctgttttg caaattcatt catcaagaat ggaaaacaga aagtcagaat
     3661 cgccatggga atcaaaacgc gccaatcgcg gaactggtct cgcagctcac gttgggctag
     3721 aagccaaaca ggtcttaatt tattgaacat attcttttcc ctttgcagtt gccatcacct
     3781 tcaagtagac ttgttccagt gtgcgcgttt cttcctgaaa ggcaactaca ggcgcggaca
     3841 aggaagtgag ccgctgaatc aattctggat ttgactctgc tggcgcttcc acccggactt
     3901 tcaaactggt agctgtcttc tcgagtagtt ccatcccttt gggcatttcc aacctactcg
     3961 catcccaagc agatgcaaat ttgatgacat attcaggagc ccccaaaaca cctgacttca
     4021 actcatctaa tgagccttga atcaaaatct ttccacggta aataatcgcg atcttatctg
     4081 caagaagttc cacttcggtg aggttgtgag agcacaacat gatggtcctt tgcgtagact
     4141 tgagcctcgc aatttcatct ctcaccaacc gcgcagactc ggggtccatg gcagaggtgg
     4201 gctcatccaa aagtaagacg ctgggttcgt gcatcattgc gcgagcaagc gctaattttt
     4261 ggcgcatccc cttggaatat tccccgatac ggcgtttgga ggcttctgcc aagccaaagt
     4321 attcaagcag gttttcggta cgtgatttac gaagggaagg gtcgaggtta tagacttgcc
     4381 caaaaaaatc catgtactct gaaccggtca tacgcatgta caaaccgtga tgttcagtga
     4441 gcacgcccac cgaagcacgg acgtcatgcc cgttttttgt cacatcgtag ccagctacac
     4501 gtgcagagcc gcggcttggc tgcaataagg cagtcaacat acgcacggtc gttgttttgc
     4561 ctgcgccatt ttgccccaac aaggcaagga tctgcccggc accaatggag agatttactc
     4621 catcgacagc cataaaatca tggaattgtt tactaagatc gttggtctca atcatgaggt
     4681 atccttttct actaagaaag aggctacgcc cattttatta cccatccccc acggctttcc
     4741 ttctcaaatc ccttacaaaa ctggtgcaag ccttctatgc ccaaggatgc ttatagagac
     4801 aatgaaaaga atagcaaaaa atagttgatt tgcattgacg ccattgacca atgccacgtc
     4861 gaggcgaagg ttctcaagca gaaaacgaac taaggaatag aatgctaaag aaccgagata
     4921 gatatcacca gcaaagaagg tatccgcaaa tttgctgcga gaaagccaaa ataacaagac
     4981 aaagcccaga aacacaagaa tggcttcata agcaaataag ggatggtaaa actcaatttg
     5041 ctcgtaacca agcaaacgat gatctggctg aataaaaatt gcccaaggca aattcgttgg
     5101 caagccgtag agttcttgat tgaaataatt cccaatgcgc cctatggctt gtgccaataa
     5161 catacccgga gcaagcgcat ctgccacttc ccaaaatgag aattcatatt tgcgagatgc
     5221 taaccacaaa gcaaaagctc caccaaggat ggcaccggga ataccaaagc cgcccgtcca
     5281 aacggagagg atatctaagg ggtgagaaag ataatgactg gtggtcagac caagctgaac
     5341 ggaggaaaga ggcggagtga acacatgcca aagcctggca ccaatccatg accacaccgt
     5401 cacagggacg aaaaggtagt agataagctc cgcatcttga aagcggcgtt tggcttcaat
     5461 ggctgcgacc aaggcaccaa ccgcaatgcc caaagcgatc aataacccgt tccagcggat
     5521 cagtaaaaaa ccgaggttta agccgtctgt cacttggctt ccttggattc ttttgatttc
     5581 ttaatgcgtt cccgcccttg tgcgcggaca ttcaagatgc gttggaaagc agtaatattt
     5641 gccaatactg caatgataat caccgcggcg aacggctggt tgaaaaccag taaaggaatc
     5701 aacacaatat atcgttcgag gcgcgtgaga attccaactt tagcagtaaa gcccaagcct
     5761 tcagcgcggg ctttgacgta ggagaccaaa accgaacctg ccgcagcaaa gaaggagatc
     5821 atcacacccg ggtagtcagc ttttgtcaga aagtaataga ccaaaccgcc aaaagtgacc
     5881 aactctgcat agcgatcact gaccgaatca acaaaaccgc caaaatcact gggttcacca
     5941 cgcaaacgtg ccatcgttcc atcaaaggca tcgactagta cagacgccag caaaatgaaa
     6001 gcgccggttg tcatatgccc ttgcgaaatg acataggcac caaccgttgt ccctgctaaa
     6061 cctaataatg tgatggtgtt cggggtaagc ccaatcccgt tgaggaaatt cccaatcggg
     6121 tcgagaatac ctttaaatat gatacgggcg cggtctgaga aagttggttt tgtattcata
     6181 tctacgtatt tttatcgttc ttcttggtta cctatttcat cttcgtcact gataagcact
     6241 tcacgttctt tgccgccgcc ttgagacggg ccaacgacac ccattgtttc taattggtct
     6301 aacaaacgag ccgcgcgtgg gtagccaatg cgcaagcgac gttgaagcat agaagcagaa
     6361 gcacgttgtt gttggcggac aatggaaatg gctttttcca ccaaaccatc gtcctcatct
     6421 tcttccggtt ctttcaacaa tgtttcccaa ggtggaactt caaccgcatc agtatcccaa
     6481 ttcttttgcc aatacgcaat gatacgttcg atctcagtat ctgtgatcaa tacgccctgt
     6541 gcgcggacgg gtgttcccac ttctgggttg aggaagagca tatcaccgcg accaagcaaa
     6601 gactcggcgc cggtgtaatc gagaataacg cggctatcgg tgcctgatgc aactgcaaag
     6661 gcaagacgcg ccgggaagtt cgctttgata agacccgtca ctacatcggt ggatggacgt
     6721 tgggtcgcaa ccacaaggtg aattcctgta gcacgcgcca tttgtgccaa gcggacgagg
     6781 ttatgttcgg tctggtctgg cgcagacatc atcaagtctg cgagttcatc gatgatgact
     6841 acgatgcgtg ggagttgttc cccatcggca gaacgtgaga ccttcttgtt gtacgaattc
     6901 aggtcacggg cgtgcatcga ttcgagcaag cgatagcgat gctccatttc aacgaccacc
     6961 caacgcaaca caccaagaat gcgttcaagg ttggtctcca cttttccgta cagatgtggc
     7021 aagccattga aacggatcaa ttccaccatc ttg
//
LOCUS       DAOREF010000016         6944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5564_length_6944_cov_4.422413, whole genome shotgun sequence.
ACCESSION   DAOREF010000016 DAOREF010000000
VERSION     DAOREF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5564_length_6944_cov_4.422413"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKI33_00645"
     CDS             complement(<1..1099)
                     /locus_tag="PKI33_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35532.1"
                     /translation="MLKTFIIGIKDVKLAFRDRAALIFMLLAPFLLTLGMGLVTGRFS
                     GDNSSGISDIPVVIVNQDDGQLSKALVDLFYSEDLANLVEPSLGTSPEAARQMVDDDE
                     VATAIIIPAGFTDSIIPPTEMGMTIYVGPKLIEMYANPSRPTSAGIIKSIVDEFMSRV
                     DESTVSGQTAIQQLLTSGRVPPEQAQAAGEELNQRLTNSQQDNTLAIKLNATTADGEE
                     IKFDVLAYLAPGMALMFLMFTVSYGGRSILAEKSQGTLPRLLVSPTNSTQILGGKVFG
                     IFLTGVAQMLILIGGTSLMFQLKWGDTLGVLVLVFAAVFGATGWGMLITALAHTPAQV
                     GSVGSATMLIFGILGGSFIQLDNMPATVQTLS"
     gene            complement(1104..2048)
                     /locus_tag="PKI33_00650"
     CDS             complement(1104..2048)
                     /locus_tag="PKI33_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM35533.1"
                     /translation="MASILEVQNLEKNYGDFKAVKGISFDIKEGEIFSLLGPNGAGKT
                     TTISMLSTLYTPTAGDATIAGHSITKAPMAVRNAIGVVPQDLALYEDLNARENLTFWG
                     QMYGLGGSALKNRVEEVLNQIGLVDKAKDRVKTYSGGMKRRVNIGVGLLHKPKLLFMD
                     EPTVGIDPQSRRAILDTVKDLNKQGMTVLYTTHYMEEAEELSNRVGIIDHGELIAIGT
                     QKELTQQVGETETLILHISENEDPEALVTAFKGIKGVLEAKSVDHEISVITPSAADVM
                     APVVTKANERGIKIHSIDIREPNLEAVFLHLTGRALRD"
     gene            complement(2065..2619)
                     /locus_tag="PKI33_00655"
     CDS             complement(2065..2619)
                     /locus_tag="PKI33_00655"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNM35534.1"
                     /translation="MLKYALLGFLSYSPLTGYELKQRMDSSTNNFWNANLSQIYTSLK
                     SLEEDGWVKSKIQAQDDKPDKRIYTLTDEGLNSLKAWLEEPITDPEQRKSTFLLKLFF
                     SAKVDKETSLAQLRVSKNLHQREKERYKSEAQTSIKDIVATYPSMKKEAIFWEATRRF
                     GELYEEMYVHWLSETIELIENKMK"
     gene            complement(2768..3835)
                     /locus_tag="PKI33_00660"
     CDS             complement(2768..3835)
                     /locus_tag="PKI33_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNM35535.1"
                     /translation="MKKIRARDLGIPFEGTPGAFNAITDVPGITVGYSTIIEGTDART
                     GVTIIHPRSMDNPTPVFAGVHSFNGNGEMTGAAWVDEGGILEGPIGLTNTHSVGVVHH
                     ALIKWQVEHHKLTQNWLTPVVAETADAWLNNMNGFFVQEHHVLEAINSAKSGEIDEGN
                     VGGGTGMLCYEFKGGTGTSSRKLPEKLGGWTVGAIAQCNFGRRYQLTIAGVPIGHHLT
                     DDAPATNGENPFTQDDGSLIVIIATDAPLLPHQLKRVAKRVSLGMSRTGAIGGNGSGD
                     IFLAFSTANSEAAKGLGRTQLEALPNGQLDPIFAATAYATEEAIVNALVAAEDMTGHE
                     ISAKALPHEDVKELLKKHGRL"
     gene            3957..4448
                     /locus_tag="PKI33_00665"
     CDS             3957..4448
                     /locus_tag="PKI33_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNM35536.1"
                     /translation="MANDTVKDGLVVSMEYKLTVDGEVLDSSDEAGPLQFLAGYGNII
                     PGLENEMMGMKIGDSKNVTVQPADGYGEFEDDAFVDVPRTEFPADMEIEEGMELHVTD
                     EDGNHQAAYVAEFDDKTVKLDFNHPLAGAVLDFYVKVVALREPTDEELDHGHVHEGDG
                     HHH"
     gene            complement(4482..5093)
                     /locus_tag="PKI33_00670"
     CDS             complement(4482..5093)
                     /locus_tag="PKI33_00670"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNM35537.1"
                     /translation="MTDTISGFFLTQVINYGAPLFALTLFLGAVGLPVGASLMVIAVG
                     AFSQQGYFEWTTMALLGLLGAALGDALSFGMGHYGKGWVEKRFGTSPTWKNAQSYFDK
                     RAWVAIFLTRFLVTALAIPTNLIAGGSGYKFQRFMLYDVLGEMTWIILYGGLGYAFGS
                     QWEVVYDFIGNFGGFVLGAVILGAGIWMARNQFNKPKQEQLAV"
     gene            complement(5090..5518)
                     /locus_tag="PKI33_00675"
     CDS             complement(5090..5518)
                     /locus_tag="PKI33_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35538.1"
                     /translation="MTKQRILTTGLIVIGLVVVIFFGMNTLRAFKHMRGHGPFNGKPP
                     TANQTDVELIRDWMTLPYIGSMYDVPPEALFYSLGIEPGKQNGKKSLKELNDEFFPEQ
                     PDLAISQVKDTIKAFQTQERPPEPFPPVAPTPPSPPTQNP"
     gene            complement(5609..6874)
                     /gene="obgE"
                     /locus_tag="PKI33_00680"
     CDS             complement(5609..6874)
                     /gene="obgE"
                     /locus_tag="PKI33_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNM35539.1"
                     /translation="MFIDQVNIHVKSGKGGDGMVHFRREKYEPRGGPDGGDGGKGGDV
                     IFEVKPILNSLSHFKPKQSFAAENGINGGGSQMTGRGGKDLTIHVPPGTVIYDAETGA
                     LLGDLTDPGQQLIVCKGGRGGRGNQHFATSRNQAPRTAERGEPHEEKMLRLELKLIAD
                     IGIIGLPNAGKSTLLSVLTNAKPKIGDYPFTTLEPNLGVAKIDEEATVVLADIPGLIE
                     GAHEGAGLGHDFLRHIQRTRVLIHMIDGLSEDPLADFSQINTELSLFDTKLGKKPQVV
                     VINKIDQPDVQARLKEIQTSFKKKKVEIITASAMARNNTRDVLLTAYRMLQELPPEQL
                     GEEALPVYKPEVDPNLFDIHREDGDKWRVSGVAIERAAKMTYWEHFGSVRRFQRLMQK
                     LGVDKALREAGVQDGDTVFVGDFELEWQE"
BASE COUNT         1689 a         1665 c         1684 g         1906 t
ORIGIN      
        1 ggctcagggt ttgaacggtt gcgggcatgt tatccaattg gatgaaactt ccgccgagga
       61 tgccgaagat caacatcgtg gcagagccaa cactgcctac ttgtgcaggc gtatgagcca
      121 gcgcagtgat aagcatgccc cagccggtag caccgaagac agccgcaaat accaacacaa
      181 gtacaccaag ggtatcaccc catttcaatt gaaacatgag cgatgtgccg ccgattagaa
      241 tcaacatttg tgctacgccg gtgaggaaga taccaaagac cttgccacca aggatttgcg
      301 tggagttggt gggggagacc aataggcgtg gcaaagtgcc ttgcgacttt tcggcaagaa
      361 ttgaacgccc gccataactg acggtgaaca tcaggaacat caatgccatg cctggagcaa
      421 gataggcaag cacatcgaac ttaatttctt cgccatctgc ggtggttgcg ttaagcttga
      481 tcgcgagcgt gttatcttgc tggctatttg tgagtcgttg atttagttct tcaccagccg
      541 cttgcgcctg ctctggtgga acacgtccac ttgtgagtaa ttgctgaatg gcagtctgcc
      601 cgctaacggt gctttcatcc acgcggctca tgaactcgtc cacgatcgat ttgatgatgc
      661 ctgcgctggt tgggcgtgat ggattggcat acatctcgat cagctttggt ccgacgtaaa
      721 tggtcatgcc catctcagtg ggcgggatga tgctatctgt aaacccggca gggatgatga
      781 tggcagtagc aacttcgtca tcgtccacca tctgacgtgc cgcctcagga ctcgtgccga
      841 ggcttggctc aaccaagtta gccaagtctt cgctgtagaa caggtccacc aacgccttgc
      901 tcagctgtcc atcatcctga ttgacaatca ccactggaat atctgaaatg ccagatgaat
      961 tgtctccgct aaaacgcccg gtgaccagcc ccatgcccaa ggtgagtagg aagggcgcta
     1021 atagcatgaa gatcaatgcc gcacggtcgc gaaaggcgag ttttacatct ttgatgccga
     1081 taataaatgt tttgagcatg atgttaatcg cgcagtgcgc gccctgtgag atgtaagaag
     1141 acggcttcca aatttggttc gcgaatatca atcgagtgga tcttgattcc gcgttcattg
     1201 gctttggtca caaccggagc cataacgtct gctgcagagg gtgtgatgac ggaaatttca
     1261 tggtcaaccg atttggcttc gagcacgccc ttaatgcctt tgaatgccgt cactaaagct
     1321 tcggggtctt cattctcgct aatgtgcaga atcaacgttt cggtttcgcc aacttgttgg
     1381 gtgagttctt tttgagtacc aatggcgatc aactcaccat ggtcaataat gcccacgcgg
     1441 ttggagagtt cttcggcttc ttccatgtaa tgagtggtgt agaggacggt catgccttgt
     1501 ttgttcaagt cttttaccgt gtcgagaatg gcacggcgag attgtgggtc gatacccacc
     1561 gttggctcgt ccatgaagag cagctttggt ttgtggagca agcccacccc aatgttgacg
     1621 cggcgcttca tcccgcccga atacgttttg acacgatcct tggctttatc caccagcccg
     1681 atctgattca acacttcttc cacgcggttc ttaagagccg agccgcctag cccatacatt
     1741 tgcccccaaa aagtgaggtt ttcgcgagca ttgaggtctt cgtatagggc aaggtcttgc
     1801 ggtacgacac caatggcatt ccgcaccgcc atgggggctt tggtaatgga gtgacccgca
     1861 atggtcgcat cgccagcggt gggggtgtag agtgtggaaa gcattgagat ggtggtggtc
     1921 ttcccggcgc cgttcggtcc gagtaagctg aagatttcgc cttctttgat atcaaacgaa
     1981 atccctttta cagctttaaa gtcaccgtag tttttttcga ggttttgtac ttccaatata
     2041 gatgccatga ttgttccttt gtgttcactt cattttgttt tcaataagtt cgatcgtttc
     2101 agataaccaa tgtacgtaca tctcttcata aagttctcca aatcttcgcg tggcttccca
     2161 gaaaatggct tcttttttca tacttgggta ggttgcgacg atgtctttaa tacttgtttg
     2221 tgcttcagat ttgtatcgtt ctttttcgcg ctggtgcagg tttttggata ccctaagctg
     2281 ggcaagggat gtttctttat ccacctttgc cgagaagaaa agttttagca agaacgtgct
     2341 tttcctttgc tcagggtcgg taataggctc ctcgagccac gcctttagac tgttaagccc
     2401 ttcatcggtc aacgtgtaga tgcgcttatc cggtttatcg tcttgagcct gaatctttga
     2461 tttaacccag ccatcctcct ctagagactt caacgaagtg tagatctgac taaggttcgc
     2521 attccagaaa ttgtttgtag aactatccat acgctgtttt agctcgtacc ccgttaaagg
     2581 ggaatagctg agaaacccga gtaaggcata tttgagcata aggcatcctt tatatataaa
     2641 tttttatata taactgttta tatattatag gttttgtttt gtcaagggct cttaaactac
     2701 aaaccctagt cataaacgac tagggtttgt agaattttcg tatagtcagt taagcggatt
     2761 tactgattta taacctgccg tgtttcttca agagttcttt cacatcttca tgtggcaaag
     2821 ccttggcgct gatctcatgc cctgtcatat cttcagctgc aacaagcgcg ttgacgatcg
     2881 cttcttctgt tgcataagct gtggcggcaa agatcgggtc aagttgaccg ttcggcaatg
     2941 cctcaagttg ggtccgtccc agtccctttg ccgcttcaga attcgctgtg gaaaatgcaa
     3001 ggaaaatatc accagagcca tttccgccaa ttgcgcccgt acgactcatt ccaagagaga
     3061 cccgctttgc gacacgtttc agttgatgtg gtagaagtgg ggcgtcggtt gcaatgatga
     3121 cgatcaatga accatcatcc tgcgtaaacg gattttcacc attggtggca ggggcgtcat
     3181 ctgttaggtg atgcccaata ggtacgcctg caatcgtaag ttgataacgt ctgccaaagt
     3241 tacattgcgc tatcgcgccg actgtccaac cgccaagttt ttcgggcagt tttctggatg
     3301 atgttcccgt tccgcccttg aattcgtagc atagcatacc cgtcccaccg cctacgttgc
     3361 cttcatcgat ctcacccgat ttggcagagt tgatcgcctc aagaacatgg tgttcttgta
     3421 cgaaaaaacc attcatgtta ttgagccagg catctgcggt ttcagctaca acgggggtta
     3481 accaattttg agttaattta tggtgttcaa cctgccactt gatcaaggca tgatgaacaa
     3541 cgccaacgct atgcgtgttt gttaatccaa taggaccttc caaaataccg ccctcatcta
     3601 cccaggcagc cccggtcatc tcaccattgc cattaaaaga atggactccg gcgaataccg
     3661 gggttgggtt atccatactg cgtggatgaa tgatcgtcac accggtgcgt gcatctgtcc
     3721 cttcgataat agtggagtat ccaaccgtaa taccaggcac gtctgtgatt gcgttgaatg
     3781 ccccaggtgt tccctcaaaa gggatgccca gatcgcgggc acgtattttt ttcatacatt
     3841 ctccttggca aagcattgaa aagacgccaa cattatagcc gaaggggttc tgtggatgcc
     3901 ttgcagtcta tttattgctg gtacaatggg aacattaaat tcgaaaggat acaagtatgg
     3961 caaacgatac agtaaaagac ggtttggtgg tttccatgga atacaaactg accgtagacg
     4021 gcgaagtttt ggactcttct gacgaagcag gtcctttgca gtttctggcg gggtacggca
     4081 atatcattcc cggtttggaa aatgaaatga tgggaatgaa gatcggcgac agcaaaaatg
     4141 tcacagttca accagccgat ggatatggcg agttcgaaga cgatgctttc gttgatgttc
     4201 cacgcactga attccctgct gatatggaaa tagaagaagg tatggaattg cacgttaccg
     4261 atgaagatgg taatcatcag gctgcgtatg tggctgaatt cgatgacaag accgtcaagc
     4321 tcgatttcaa tcatcccctt gctggcgctg tgttggattt ctatgtcaaa gttgtagctc
     4381 tccgtgagcc aacggacgaa gaactcgatc acggtcatgt ccacgagggc gatggacatc
     4441 atcactaata aaaaaacatc ctctgtatag aggatgtttt tttatacagc cagttgttct
     4501 tgtttgggtt tattgaattg atttcttgcc atccaaatgc cagcacctaa gatcacagca
     4561 cctaatacga acccaccgaa gtttccaata aaatcataga ccacttccca ttgagaccca
     4621 aatgcatatc ccaacccgcc atacagaatg atccaggtca tttcgccgag aacatcatat
     4681 agcataaaac gttgaaactt atatccgctg ccaccagcaa tcaggtttgt gggaattgcc
     4741 aacgccgtta ccaaaaagcg agtgagaaaa atggctaccc aggcacgttt atcaaaatag
     4801 gattgagcgt tcttccaagt gggggaggtg ccaaagcgct tttcaaccca gccttttcca
     4861 taatgaccca ttccaaagct gagagcatct cctagtgcag ccccgagcag gcctagaagt
     4921 gccatcgtgg tccattcgaa atatccttgt tgagagaaag cgccaacagc aataaccatc
     4981 agagaggcac ctacgggtag cccaaccgct ccaagaaaaa gcgtcaacgc aaaaagaggc
     5041 gccccgtagt taataacttg tgtaaggaaa aatccagaga tcgtatcggt catgggtttt
     5101 gagtcggcgg tgacggtgga gtcggtgcaa caggtgggaa tggttccgga ggacgttcct
     5161 gtgtttggaa tgccttgatc gtatctttta cttgcgagat cgccaggtct ggttgttcag
     5221 ggaagaattc atcgttcaat tctttcaaac ttttcttccc gttttgtttt ccgggttcaa
     5281 tccctaaact gtagaacaag gcttctggcg gaacatcata catactgcca atgtagggaa
     5341 gcgtcatcca atctcggatc aattccacat cagtttgatt cgctgtaggt ggtttgccat
     5401 tgaatggacc atgtccgcgc atgtgtttaa aggcgcgaag agtattcatg ccaaaaaaga
     5461 taacgacaac caatcctatg acgatcaacc cggttgttaa aatgcgttgt ttagtcatat
     5521 gcgtatttta tccctgcttt caggttcaaa ttgtaaagaa ataatgtgga atcaaaaagg
     5581 acacaccatg tggtgtgtcc ttttatcgtt actcttgcca ttccaattca aaatcgccta
     5641 cgaaaaccgt atcgccatct tgaaccccgg cttcgcgtaa tgccttatcc acgccgagtt
     5701 tttgcatcaa acgttggaag cgccgcacag agccaaagtg ttcccaatac gtcatctttg
     5761 cagcacgttc aatcgctacc cccgatactc tccacttatc tccatcttct ctatggatat
     5821 cgaacaagtt cgggtctact tccggtttgt agacgggcaa cgcctcttcc ccgagttgtt
     5881 ctggtggcag ttcctgaagc atacgatacg ctgtgagcaa cacatctcgt gtgttgttgc
     5941 gtgccatggc tgaggcagtg atgatttcca cttttttctt tttgaagctg gtttgaattt
     6001 cttttaatcg cgcttgcaca tcaggctggt caatcttatt gataaccaca acctgcggct
     6061 ttttgcctag ctttgtatca aagagcgaaa gctcagtatt gatctgactg aaatcggcga
     6121 gtgggtcttc tgataacccg tcaatcatat gaatgaggac gcgagtgcgt tgaatatggc
     6181 gtaaaaagtc gtgccccaac cctgcacctt catgagcgcc ttcgatcaag ccagggatat
     6241 ctgccagcac aacggtggct tcttcatcaa ttttggcaac acccaggttg ggttcgagag
     6301 ttgtgaaggg atagtcaccg atcttgggtt tggcgttcgt cagcacggag aggagagtgg
     6361 atttcccggc gttgggtaag ccaatgatgc caatatcagc aatcagctta agttcgagcc
     6421 gcagcatttt ttcctcatga ggttcgccgc gttcggcggt ccttggggct tggtttcgtg
     6481 aggtggcaaa atgctggttg ccacgtccac cccgcccgcc cttgcagacg atgagttgct
     6541 gtcctgggtc tgtgaggtcg ccgagcaatg ctccggtttc cgcatcatag atgacggttc
     6601 ccggtggaac atgaatggtc aggtctttgc cgccacgccc ggtcatttgc gaaccgccgc
     6661 cgttgatgcc attttcggct gcaaaagatt gcttgggttt aaagtgagag agggaattaa
     6721 gaatgggttt cacttcaaag atgacatcgc cgcctttgcc cccgtctccg ccgtccggac
     6781 cgcccctagg ttcgtatttt tcacggcgaa aatgcaccat accatcgccg ccttttccag
     6841 attttacatg aatatttact tggtcaatga acattccccc attataatgg ctataagtga
     6901 tctttcgagg gaatacaaat taaggggtat ttgcttgaat agac
//
LOCUS       DAOREF010000017         6932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5581_length_6932_cov_4.200378, whole genome shotgun sequence.
ACCESSION   DAOREF010000017 DAOREF010000000
VERSION     DAOREF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5581_length_6932_cov_4.200378"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2285
                     /locus_tag="PKI33_00685"
     CDS             <1..2285
                     /locus_tag="PKI33_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNM35540.1"
                     /translation="MSTSLAQVIVLLFIGVFYLPVIFFAIQRREEGQGAATWLVVLYA
                     LLAMVINIAEAIWQDRGDNSFLFQELQTYISFTLIIILMITIQVFLKRELWWAWLAYF
                     GVWVIGLALILTNALNLPDTLWTNGELILYRSRLGPAWVMLGWLILSIVMILNLFNAQ
                     HQSRQPLLRSRLGYWWAPVVFTLVNDILLFSGILIIGQPVRLLVAIGMAYVVGTHYVP
                     DLKNITRRAMIYVASTIGIVGFYIAGFLIIQALFGGNPSYSPLLAGAVVALFIALIFA
                     PLTDLVSRAINIWTKGDQYDASLTIHQYSESISNILDMDRLASIAIGIMLEAMQIERG
                     FLFLVDSERQADGRKTYKIRSARSPEERQLVAVELEEDGLIASYFIREQKPLLQYDLD
                     LLPAFRAASTVEKNWFTELRSEVYIPIFAKKQWIGLLAFGSKLSGNRYTREDLSVLSA
                     HANQTAVALENARLVDNLMRLNQELRQARRTLEKNNQELQRIDQAKSDFISIASHELR
                     TPLTVIKGYTEMLMDDPKLEKGQKSMMKGIHDGTIRLHEVMDSMFDIAQIDARSLKPH
                     LQPVDVGQLIHQVCLEHAKTVKEREQILTIKAQAIPLVKADSHLLEKLFHHLIRNAIK
                     FTPNNGWITITAKHIPAIINLPNGGIEIIVSDTGVGVDPNLREVIFSKFYQPGELGKH
                     STSKSRFKGNGAGLGLALSKGIIEAHGGRIWVESPGYDEVNFPGSQFHIILPLTKLEN
                     GEVQRTSEPLTIELGKAKEA"
     gene            complement(2329..3459)
                     /locus_tag="PKI33_00690"
     CDS             complement(2329..3459)
                     /locus_tag="PKI33_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35541.1"
                     /translation="MLVIEKVDTENKKQVKRFVELPPRLYADCPQWVPPLNVDAYNQL
                     NRKKHPFHEHSDVDFFLAVRDGLDVGRIAVIENKPFNQYHQEKTADFYLFECEDDLEA
                     ATALFNTAFEWAKSRGLTKMVGPKGMGPLDGYGVLVFGHEHRQTMTMLNYNHAYYQGL
                     VEAQGFTKEVDFVSCYLPADKFQMPERVKKISERVMQRGHLEIKTFKSKKDLLSWADR
                     IGTAYNNAFVNNWEYYPLSQREIDFVVENIMTIADHRLIKIIVHEQDVVGFLFAFHDV
                     SAAMQRAKGKLFPFGLLGILMELRRTNTVAVNGMGILPEFQGHGGNALLYYAMGDTLL
                     NFGQFTHVEMTQVAETTEQMRADLKNLNGVEYKNNRVYRKLL"
     gene            3618..4880
                     /locus_tag="PKI33_00695"
     CDS             3618..4880
                     /locus_tag="PKI33_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNM35542.1"
                     /translation="MTGTTHQQMLAKYAEAIVKVGLNIRAGQRLIITAAGTRGVPNQF
                     APLVREITKAAYGVGAKYVDVIFADEEMMRIRAQHAPKGSFGEYSKWQIQGILDMINN
                     GDALLSIGGSNPDLLGGLDPEVVGQMQRAHLENWSPISEKVTTNAINWCVVAAAGEAW
                     AHKIFPDLPLNQAQEKLWQAIFETTRIDQPDPVGAWSKHIASLRERALYLQSKQYTAL
                     HYTAPGTDFTVGLPSGHLWISAQSMAQNGVAFTANMPTEEVFTLPDRNRADGVISATF
                     PLSYGGTLIENFQVHFENGRITKVTAKKGEAALQKLVDTDEGSHRLGEIALVPASSPI
                     GKRGHLFYNTLYDENASCHIAIGRAYRFTLAGGTELNDDEFTAAGGNISLNHVDFMIG
                     SPQMDIDGITKDGKHEPVMRQGEWAISL"
     gene            5001..6236
                     /locus_tag="PKI33_00700"
     CDS             5001..6236
                     /locus_tag="PKI33_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNM35543.1"
                     /translation="MNETFQTSLEKYADIIVNIGLNLHKEQRLNIAAGLPDAELVRLV
                     AKKAYQLGSPFVDVRWIDERLTRIRFENSKPEHLVEVPQWQIDREHEYGSRGDALLSF
                     ASQDPNLLEGISPDLIGRYRKAISEKYEPVTKYRNENTQTWCVVSTATPAWAQKVFPD
                     LPVEQAQEKLWEAIFDACRVNTADPVQAWREHAQKLHRQCDYLNEKRYAGLHYKAPGT
                     DLTLGLPDNHLWIGASGKTQNGIEYIPNLPTEEVFSMPHREKTEGYVTSSRPLILSGT
                     MIDEFTLTFENGRVSKVTAKQGEENIRKLLETDENASRFGEAALVPDSSPISQRKHLF
                     YNTLFDENASSHLALGTAYRYNVKGGTSMTKEEFIAAGGNDSLIHVDFMIGSDQMDID
                     GIRADGTREAVMRKGEWAF"
     gene            6338..6916
                     /locus_tag="PKI33_00705"
     CDS             6338..6916
                     /locus_tag="PKI33_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35544.1"
                     /translation="MPRKPALNTDEILNSEYQYIAQNVFQSNEDRSRVTSFYFVSVGS
                     FVAAILGTRFEPDQSTISLAFFFLFTILTFMGALTIAQLARLRAAWHEAVEAMNQIKD
                     YYIKHMPELEPAFKWRKRQIPPTNKPYSIANLMAVEVALLSALTTGAGIYFLMTYIGI
                     SNWVTWSAVIVSVIFGYIAQWTWYRYLLVDNQ"
BASE COUNT         1853 a         1779 c         1531 g         1769 t
ORIGIN      
        1 tcatgagcac atcgctagcc caagtcatcg tcctattgtt cattggcgtg ttctatctgc
       61 cggtcatctt ttttgctata cagcgccggg aggaagggca aggcgcagcc acttggttgg
      121 ttgttttgta tgccttactt gcaatggtga tcaacattgc cgaagccatt tggcaagacc
      181 gtggggataa ctcttttctt ttccaagaac ttcaaacgta tatttcgttc acccttatca
      241 tcattttgat gatcacgatt caagtcttct tgaagcgcga actatggtgg gcttggctgg
      301 cgtattttgg agtttgggtc attgggctgg cattgatcct tacaaatgct ttaaacctgc
      361 cagatacttt atggacaaat ggtgaattga tcctgtatcg ttcgcggttg ggtcctgctt
      421 gggtcatgtt gggatggtta attttatcca tcgtgatgat cctaaactta ttcaatgcgc
      481 aacaccaaag ccgccagccg ttgcttcgca gccgattggg atattggtgg gcgcccgtcg
      541 ttttcactct cgtcaacgac attcttcttt tctctggaat tcttatcatt gggcaaccgg
      601 tgcgcttgtt ggttgcaatt ggcatggcat acgtagtagg cacacactac gtgccagacc
      661 taaagaacat tacccgtcgt gccatgatct atgttgccag cacgatcggc attgtaggct
      721 tttacatcgc tggctttttg attatccaag ctttattcgg tggcaaccca agttatagtc
      781 cgctattggc aggcgccgtt gttgcgcttt tcattgcgtt gatcttcgcg ccactcacag
      841 acttggtctc acgcgccatc aatatctgga cgaaaggcga tcaatacgac gccagcctca
      901 ccattcacca gtatagtgaa agcatcagca acatcttgga catggatcgc cttgccagca
      961 ttgccattgg cattatgctg gaagccatgc aaattgaacg cggcttccta ttcctcgttg
     1021 attctgaacg tcaggcagat ggtcgcaaga cgtataaaat tcgttcggca cgtagccctg
     1081 aagaacgaca attggtcgct gtggaattgg aagaagatgg gcttatcgct tcgtatttta
     1141 ttcgggagca aaaaccgctt cttcaatatg acctggacct gttgccggcg tttcgagctg
     1201 cttctaccgt tgagaaaaat tggtttacgg aattacggtc agaagtttac attcccattt
     1261 ttgccaaaaa acaatggatc ggtctgctgg cattcggttc aaaattaagc ggcaatcgtt
     1321 acacccgaga agacctttcc gttcttagcg cacatgccaa ccagacagcc gttgcgcttg
     1381 aaaacgcccg cctcgtggac aatctcatgc gcttgaacca agaattacgt caggcgcgcc
     1441 gtacgcttga aaagaacaat caagaattac aacgcattga ccaggcaaaa tctgatttca
     1501 tcagcattgc atctcatgaa cttcgtacac cgctcaccgt catcaaaggc tacaccgaaa
     1561 tgctaatgga tgacccaaag ctagaaaagg gtcagaagtc catgatgaag ggcattcacg
     1621 atgggacgat ccgtctgcac gaagtgatgg actcgatgtt cgacatcgct cagatcgatg
     1681 cacgctcgct caagccccat cttcagcccg tggatgtggg tcaattgatc catcaggtat
     1741 gtctggaaca tgccaagacc gtcaaagaac gcgaacagat tctaaccatc aaagcccaag
     1801 ccattccgct tgtcaaagcg gactcgcacc tcttggaaaa gcttttccat cacttgatcc
     1861 gcaatgccat caagttcacg ccgaacaatg gctggatcac catcaccgcc aaacacatcc
     1921 cagccatcat caacctcccc aacggtggca tcgagatcat cgtcagcgat actggtgtgg
     1981 gcgtagaccc gaacttgcgc gaggtaatct tctcgaagtt ctaccagcca ggtgaactgg
     2041 gcaagcattc cactagcaaa tcacgcttca agggcaatgg agccggttta gggctggcac
     2101 tctcaaaagg tattattgag gcgcatggtg gtcgtatctg ggtggaaagc cccggctacg
     2161 acgaggtcaa cttcccgggc agtcagttcc acatcatcct gccgttgacc aagcttgaaa
     2221 atggcgaagt gcaacgcaca agtgaacctc tcaccataga acttggaaaa gcaaaagaag
     2281 cgtaaaaaaa gagcggagat ttataaatct ccgctctttt tatcagaatt acagtaattt
     2341 gcgatacaca cgattatttt tatattccac cccattcaag ttctttaaat ctgctcgcat
     2401 ttgttcagtg gtctcagcca cttgtgtcat ttcaacatgg gtgaattgac cgaagtttaa
     2461 taatgtatct cccatcgcat aatacaacaa ggcattccca ccatgcccct gaaactccgg
     2521 caggattccc ataccgttca cagctacagt atttgtacgg cgaagttcca tcaaaatacc
     2581 caacaacccg aacggaaaga gcttgccttt cgcacgctgc atggctgcag aaacatcatg
     2641 aaacgcaaag agaaaaccaa ccacatcctg ttcatggaca atgatcttga tcaggcgatg
     2701 atcggcgata gtcatgatat tttccaccac aaaatcgatc tcgcgctgtg agagaggata
     2761 atattcccag ttattcacaa aagcgttgtt gtaagcagtg ccgattcgat ccgcccaact
     2821 caacaagtcc tttttgctct tgaatgtttt tatctctaaa tgaccccgtt gcataacacg
     2881 ttcagagatc tttttaactc gttcaggcat ttggaattta tccgcgggta aataacaaga
     2941 gacaaagtca acttcttttg tgaaaccctg tgcttcaacc aatccctgat aataagcatg
     3001 gttgtaattg agcatagtca tggtctgacg gtgttcatgc ccgaaaacca acactccata
     3061 cccatctaac ggacccatcc ctttcggtcc gaccatcttg gttaatccgc gtgattttgc
     3121 ccactcaaac gctgtgttga acaatgccgt agccgcttca aggtcatcct cacactcgaa
     3181 taaataaaaa tctgccgtct tttcttgatg atattggttg aaaggtttgt tttcaattac
     3241 agcaatccgc cctacatcca gtccgtcacg aaccgctagg aaaaaatcca catcagaatg
     3301 ttcatggaag ggatgcttct ttcgattgag ctgattgtac gcatccacgt tcaatggcgg
     3361 cacccactgc gggcaatccg catagaggcg gggtggtaac tccacgaacc gtttgacctg
     3421 ctttttattc tcggtatcca ctttctcgat aacaagcatg gttttgctcc tatagaggct
     3481 aggtttcagc caagtatatc ccttccaccc cattttaaaa ggtgggtgtg cattgctttt
     3541 gtaatggata tccctcgtgc aaaaacccct gtcgccatgc tatacttgta actatctccc
     3601 aaattaactg aggatttatg acaggaacta cccaccaaca aatgctggca aaatatgccg
     3661 aagccattgt aaaagtaggt ctaaatattc gagccgggca acgtttgatc atcacagctg
     3721 cagggacgcg cggtgtacct aaccaatttg caccattagt aagagaaatt accaaagccg
     3781 cgtatggtgt tggcgcaaaa tatgtagatg tcatctttgc agatgaagaa atgatgcgca
     3841 tccgcgctca acatgccccg aaaggcagct ttggagaata ttccaaatgg caaattcaag
     3901 gcatcttgga catgatcaac aatggcgatg ccttgctttc catcggcggc tcaaaccccg
     3961 acctacttgg tgggcttgac cctgaagttg ttgggcagat gcaacgagcc catttggaaa
     4021 attggagtcc cattagtgaa aaagtgacca ccaatgccat caactggtgt gtagttgccg
     4081 ccgctggtga agcatgggcg cataagattt tcccagacct tcctctgaac caagcgcaag
     4141 aaaaactttg gcaagccatc tttgaaacaa ctcgtattga ccaacccgac cccgttggtg
     4201 catggagcaa acacatcgcc tctttacgcg aacgggcttt gtatctgcaa tccaagcaat
     4261 acacagcgtt gcattacaca gcccccggca ccgactttac cgtcggcttg cccagcggac
     4321 atctctggat cagtgcccaa tcgatggcgc agaatggtgt tgcttttact gctaatatgc
     4381 ctaccgaaga agtctttact ctgccagatc gcaaccgtgc agatggtgta atttctgcca
     4441 ccttcccgct cagctatggc ggcactttga tcgaaaactt ccaagtccac tttgagaatg
     4501 ggcgtattac caaggtcaca gccaagaaag gtgaagccgc ccttcaaaaa ttagtggata
     4561 ccgatgaagg ttcacatcgc ctcggtgaaa tcgccctcgt cccagcatca tcccccattg
     4621 gcaaacgcgg acatctcttc tacaacacgc tttatgatga aaacgcctcc tgtcacattg
     4681 ccattggacg cgcctaccgc ttcactctcg ccggaggaac tgagttgaac gacgatgaat
     4741 ttaccgcggc tggaggcaac atcagcctca accacgttga cttcatgatc ggttctccgc
     4801 aaatggacat tgatggcatt accaaagacg gcaaacatga gccggtgatg cgtcagggtg
     4861 aatgggcaat ctcactgtaa ccgaatcaac ccaactcccc gtcatgaggc ggggagtttt
     4921 tatgtcacca aagttggtgg tcatctgagt ccacttatgg taaaaatcat tcaacgccct
     4981 gtctatctaa aggagaaacc atgaacgaga ccttccaaac ctctcttgaa aaatacgccg
     5041 atatcattgt aaacattggt ttgaacctgc ataaagaaca acgcctaaat attgcagccg
     5101 ggttacctga tgcagagctt gttcgtttag tggcaaagaa agcttatcaa ctaggctctc
     5161 cttttgttga tgtgcgctgg atcgatgaaa ggttgacgcg catccgtttt gaaaattcga
     5221 agccggaaca cctcgttgaa gttccacagt ggcaaattga ccgcgagcac gaatatggct
     5281 ctcgcggaga tgcgctcctg agcttcgctt ctcaagaccc caatttacta gaaggcatca
     5341 gcccagattt gatcggcaga tatcgaaaag cgatttcaga aaaatacgag cctgttacca
     5401 agtatagaaa tgaaaatact caaacttggt gtgttgtttc tacagccacc cctgcttggg
     5461 cgcaaaaagt ttttccagac ttacccgtag agcaagcaca agaaaaatta tgggaagcca
     5521 tttttgacgc ctgccgtgtg aataccgcag acccagttca agcatggcgc gaacatgcac
     5581 agaaacttca tcgtcaatgt gattacttga acgagaagcg ctatgctggg ctccattaca
     5641 aagcccctgg caccgaccta acgcttgggt taccagacaa ccatctttgg atcggtgctt
     5701 ctggcaaaac tcaaaacggt attgaataca ttcccaacct gccaacagaa gaagtctttt
     5761 ccatgccgca tcgtgaaaaa acagaaggct atgtcacttc cagccgtccg ctgattctct
     5821 ctggtaccat gatcgacgaa ttcacgctca ccttcgaaaa tggacgtgtg agcaaggtca
     5881 cagctaaaca aggcgaagaa aacatccgca aacttttaga aacagacgaa aacgcctcac
     5941 gcttcgggga ggctgctttg gttcctgata gctctcccat ctctcagaga aagcacctct
     6001 tctataacac cctcttcgat gaaaatgcgt ccagccacct tgctttgggt accgcttatc
     6061 gctacaacgt caagggcggc acatcaatga ccaaagaaga attcatagcg gctggcggca
     6121 atgacagcct catccatgtg gactttatga tcgggtctga ccaaatggac attgatggca
     6181 tccgggccga tggaacgcgc gaagctgtca tgcgtaaagg tgaatgggct ttctaagcgt
     6241 aatacgtaaa aacaaaagcg gacgttgatg aaacgtccgc tttttgcatc tgtgataagc
     6301 ctatataata gaagtacctt catcaaggag caattgcatg ccacgtaaac cagcactaaa
     6361 caccgatgaa attctcaact cagaatatca atatattgcc cagaacgttt ttcaatcgaa
     6421 tgaagaccgc tcgcgggtga cctcttttta cttcgtttcg gttggctctt ttgttgccgc
     6481 cattctgggc acacgcttcg agccagatca aagcaccatc tctttagctt ttttcttcct
     6541 cttcaccatt ctaactttca tgggtgcgtt gaccattgcc cagctcgccc gtctgcgcgc
     6601 cgcgtggcat gaagcagtag aagcgatgaa tcaaattaag gattattaca tcaaacatat
     6661 gcccgagttg gagcccgcct tcaaatggcg aaagcgacag attccaccca ccaacaagcc
     6721 atatagcatt gccaatctca tggcagtgga agtggctttg ctcagcgcgc tcactaccgg
     6781 cgctggcatc tatttcctca tgacctatat cggcatttca aattgggtca catggtcagc
     6841 agtcattgtc agcgttatct ttggctacat cgctcagtgg acctggtatc gctatctact
     6901 cgtagacaat caataggaga acctcatgcc aa
//
LOCUS       DAOREF010000018         6907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5614_length_6907_cov_4.002043, whole genome shotgun sequence.
ACCESSION   DAOREF010000018 DAOREF010000000
VERSION     DAOREF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_6907_cov_4.002043"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..680)
                     /gene="nuoI"
                     /locus_tag="PKI33_00710"
     CDS             complement(159..680)
                     /gene="nuoI"
                     /locus_tag="PKI33_00710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNM35545.1"
                     /translation="MGVFDPIIELTKGLGETLRSFLREPTVTVSYPEEKRPVRPRFRG
                     RHVLKRYDNGLEKCIGCSLCAAACPADAIFVEASANTDEKRFSPGERYASTYEINMLR
                     CIYCGFCEDACPTEAIVLGDNYELSFTNRQDAIYTKDRLLEPVPTESMLTPRKVDAGV
                     FTRSVPEMKDPTD"
     gene            complement(667..1836)
                     /gene="nuoH"
                     /locus_tag="PKI33_00715"
     CDS             complement(667..1836)
                     /gene="nuoH"
                     /locus_tag="PKI33_00715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNM35546.1"
                     /translation="MDWTMWVEWVIKGFVLCLILLTGFAYLTLYERRALARIQVRVGP
                     NRAGPQGLLQPIADAVKLIFKEELTPGHVYKLVFILAPVLTVVPSLIIAAVIPLGTSF
                     SLFGRNINLYVANLNVGVLYLTSIASIAVYGIVLAGWSSNNKYAMLGGIRSSAQMISY
                     ELSLGLCFVIAIILSALGNPDGGSMNLREIVEAQKDMWFVVYQPAGALIFLIVTLAEV
                     NRAPFDMPEAEQELTAGYHAEYSGMKFALFFMAEYQKMIVISMIAATLFFGGYREFWF
                     MTNLFGGIFSVDRFWFLGPIYLMLKVVALLFFMIWVRATWPRIRYDRLMAFGWKVLLP
                     LCLILAFVTAVGILLVDFVGNPLIMWGVPVISVVFLFAFVAFINRDLRRKSHGRV"
     assembly_gap    1983..2082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2083..4468)
                     /gene="nuoG"
                     /locus_tag="PKI33_00720"
     CDS             complement(<2083..4468)
                     /gene="nuoG"
                     /locus_tag="PKI33_00720"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNM35547.1"
                     /translation="MSKQVTLTINGKQVTAPEGLSVADAAKLAGIDIPVFCHHPKLEP
                     VGMCRMCLVEIGRPVRDRATGQFVMENGQPKIQFMPKLETACTNKVEDGMVVLTESAK
                     ATDGQRGTVEFLLTSHPLDCPICDKGGECPLQNLTMAHGPGKSRFNYNEKQHLEKMVP
                     LGELIYLDRERCIQCARCIRFQSDVAGEAVLGFDERGRYTQIISLSEPGFDSVFSGNT
                     TDICPVGALTTVDFRFGARPWELKAESSICTQCPVGCNTTLNTRREAKADGEVVVKRV
                     MPRQNEEVNEIWLCDKGRFAYHYTEGKERLTKPMVRKDEKLARASWDNAIKTAAENFV
                     ANKKDFVVLASGRLSNEDLFNLKSLADTAGGEAILYSDMAGGELTQLVGVGAGTNLGK
                     MGKGDAILVIASDLYEEAPIWWLRVKQAADRGATLIVANPRQTKLDRFAKYTIRYAYG
                     DEIKTIHDFEKKSKISDEFAKAKNAVVFYGSEGLGVAGTSALASACADLLKETNHIGN
                     ANNGLIGVWQRANDQGAFEVGFRPVADLEKALKGKAVYIVGADPVGDDPNLAKALKSA
                     KFVAVQELRETATTEIADVVFPAQAFTERDGTVVSGERRVQRFYAALAPKGDSKPDYA
                     ITSMLAKQMGVILQGTSASVVFDILANSVEAFNELTYEALAQVKEQWPLVGRNDVYYG
                     GTTYENTKGLGVQLVPVAQAGVSVKIQKVRKEAALRPKEKELLAVPANKLYDLGVTVQ
                     TAEFLDQRIGETKVAMHPTTAKKFEVEDGQAVKVSFEGVQAEAVARLDETIATGV"
     gene            complement(4475..5722)
                     /gene="nuoF"
                     /locus_tag="PKI33_00725"
     CDS             complement(4475..5722)
                     /gene="nuoF"
                     /locus_tag="PKI33_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNM35548.1"
                     /translation="MAYVLLRHRDIPDIEKMSVYKKNGGFSALKNAITKMQPNDVIDV
                     VKNSGLRGRGGAGFPTGMKWSFIDKNSWPHYIVANADESEPGTFKDREIMEKNPFQFL
                     EGLALGAYAVGANAAYIYLRGEFWQVAAWLDEKIAEMEEAGYLGENLFGKDYSLKIYT
                     HLGAGAYICGEETALLESLEGKRGQPRVRPPFPPSVGLYGKPTIINNVETFTNVPMIL
                     ANGADWYKSMGTADSAGVKIFSLSGRVRKPGNYELPFGTTFRQLIYEHGMGIQEGRPI
                     KAIMPAGASSSVIVVDEKVLDTPLDYASVRTFGADLGSASVIVLDDTVDMDWVINKTI
                     HFFKHESCGKCTTCREGNHWMRNLTDRIHSGKGSGDDVKLLLNVAKQMQGKCLCALGE
                     FSTMAVVTSIERFPDDFKKAVKA"
     gene            complement(5725..6219)
                     /locus_tag="PKI33_00730"
     CDS             complement(5725..6219)
                     /locus_tag="PKI33_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNM35549.1"
                     /translation="MSLEKKYSKEVKQILAKYPPEHKRSAVMPLLYLAQREEGYVNKE
                     SMKDIASILDITETEVASIIGFYSLYHDKKAGKYRMQVCNDLPCALRGSDEFLNKLCE
                     NLGVKVGETTEDGLITIEAVTCLASCDRAPMFQTQGPDGIKYHDYMTIDRTMELIEAL
                     KGGK"
     gene            complement(6216..>6907)
                     /locus_tag="PKI33_00735"
     CDS             complement(6216..>6907)
                     /locus_tag="PKI33_00735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNM35550.1"
                     /translation="DVPVEFEKAVRDFIKIFPKRIDEYEKLLTKNPLFVDRMVDIGYL
                     SKETALSYGVTGPNLRASGVNWDLRKARPYMGYEQYDFDVPVLTEGDTYARYLVRIEE
                     FRQSLRIIEQALDKLPMGPVRSENRKFVPPPRSEIGVSMEALIHHFKLWTEGFHAPDA
                     SIYSAVESPRGELGVLLAGDGGPKPRRVHMRTPSFDNLSVIPEIVKGHLVADLVAILA
                     SIDIVLGDIDR"
BASE COUNT         1762 a         1829 c         1626 g         1590 t
ORIGIN      
        1 gaatatacag cgttctggct gaacaacatt cccagtgcgg tcgcgatcgc gacgagggaa
       61 agaacgagga agactattaa ttcagaagac atggcactcc atttacgatt ttcgactgac
      121 gatttccaaa ccaatcgaaa atctgaaatc gtaaatcgtc aatcggttgg gtctttcatc
      181 tccggcaccg aacgggtgaa cacaccagca tccactttgc gcggggtcag catggactcg
      241 gtaggaacgg gttccaaaag gcggtctttg gtatagatcg catcctgacg gttggtaaag
      301 gaaagctcgt agttatcccc tagcacgatc gcttcggtgg ggcaggcatc ttcacagaaa
      361 ccacagtaaa tgcaacgcag catattgatc tcgtaggtag aggcatagcg ttcgcctgga
      421 gagaagcgct tttcgtctgt attggctgaa gcttccacaa agatcgcatc ggcagggcag
      481 gcagccgcac acaaggaaca accgatgcac ttctccaagc cgttgtcgta acgcttcaag
      541 acatgacgtc cacggaagcg cgggcgcaca gggcgtttct cttccgggta gctcaccgtt
      601 acggttggct cacgcaggaa ggaacgtaac gtttcgccca aaccttttgt cagttcgata
      661 atgggatcaa acacgcccat gagattttct cctcagatcg cggttaataa atgcaacaaa
      721 cgcaaaaaga aaaacgaccg aaatcacagg cacaccccac ataatgagag ggttacctac
      781 aaagtcaacc agtaagatgc ctactgctgt cacaaaagcc aggattaagc acaatggcaa
      841 aagcaccttc cagccgaatg ccatcaagcg gtcgtagcgg atgcggggcc aggtagcacg
      901 cacccagatc atgaagaaca acaaggcaac taccttcaac atcaagtaga tcggtccaag
      961 gaaccagaaa cgatccacac tgaagatgcc gccaaacaaa tttgtcataa accagaattc
     1021 acgatacccg ccaaagaaca aggtggcagc aatcatggag atcacgatca tcttttgata
     1081 ttccgccatg aagaacaaag cgaatttcat acccgagtat tcggcgtggt agccagccgt
     1141 caattcttgt tccgcttcag gcatgtcgaa cggagcgcgg ttcacttcag caagcgtcac
     1201 gatcaaaaag atcaaagcgc ccgctggctg ataaaccaca aaccacatat ccttttgggc
     1261 ttccacgatc tcgcgcaaat tcatcgagcc gccatcgggg ttgccaagag cgctcaagat
     1321 aatggcgatc acaaagcaca agcctaaaga aagttcataa gagatcatct gcgccgaaga
     1381 gcggatgccg cccaacatgg catacttgtt attgctcgac catcctgcca gcacaattcc
     1441 gtacaccgca atcgatgcaa tggacgttag ataaagcaca cctacattca gattcgcaac
     1501 atacaaattg atattacgtc cgaaaaggct gaatgaagta cccagcggaa taactgccgc
     1561 aataatcagc gaaggtacca ccgtcaacac aggcgcaagg ataaagacca acttatacac
     1621 atgccccggc gtcaattctt ctttgaagat caacttcacg gcatctgcga tcggctgcaa
     1681 caaaccttgc ggacccgcac ggttcggacc aacacgcacc tgaatacgag ccagcgcgcg
     1741 gcgttcatac aacgtcaaat aggcaaaccc cgtcaaaagg atcaaacaaa gcacgaagcc
     1801 tttgatgacc cattcaaccc acattgtcca atccataatt acttcgcctt cccagatgca
     1861 gtcttcgctt gcactcgcgc caccacaggt tcatggatcg caatgcccat gctgcgtgga
     1921 ataagcacca cacccgtcgc aatagtttca tcgagcctgg ctaccgcttc tgcctgaaca
     1981 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccacaccc gtcgcaatag
     2101 tttcatcgag cctggctacc gcttctgcct gaacaccctc aaagctaacc ttcacagcct
     2161 gcccatcttc cacttcgaac ttcttcgctg tggttgggtg cattgcaact ttcgtctcgc
     2221 caatgcgctg atcaagaaac tctgcggttt ggacagtcac accgaggtca tacaatttat
     2281 tggcaggcac agccagcaat tctttttctt tgggacgaag agccgcttct ttgcggacct
     2341 tttgaatttt cactgaaacc ccagcctgag ccaccgggac gagttgaaca cccaagccct
     2401 tcgtgttctc ataagtcgtg ccgccgtaat acacatcatt acgacccacg agcggccatt
     2461 gttccttgac ctgagccaag gcttcatagg tcaattcatt gaaagcttcc acagaattcg
     2521 caagaatgtc gaacactact gaagcagaag ttccctgaag aatcacgccc atctgcttgg
     2581 caagcatgga ggtgatggca tagtctggtt tgctatcacc tttgggagca agcgccgcat
     2641 agaagcgttg tacacggcgt tcaccagaga caaccgtacc atcacgttct gtaaatgcct
     2701 gcgccgggaa gaccacatct gcaatttcag ttgttgcagt ttcacgtaac tcctgcacgg
     2761 ctacgaactt ggcactcttc aatgctttgg caaggttggg gtcatccccc actgggtctg
     2821 caccaacaat gtaaacggct ttgcccttca acgccttttc aaggtcagcc accggacgga
     2881 agccaacttc gaacgcaccc tgatcattgg cacgctgcca aacaccgatc aaaccattgt
     2941 tggcattgcc gatatgattg gtttctttca acaggtctgc gcaagctgaa gcgagtgcag
     3001 aggttcctgc cacaccgaga ccttcgctgc catagaagac gaccgcattc tttgccttcg
     3061 caaattcgtc tgagatcttg ctcttctttt caaagtcgtg aatggtcttg atttcatcac
     3121 catatgcata gcggatggtg tatttagcga aacggtcgag cttggtctgg cgggggttgg
     3181 caacgatcaa agtcgcgccg cgatccgcag cttgcttcac acgcagccac caaatgggag
     3241 cttcttcata taagtcagaa gcgattacga gaatagcatc gcctttgccc attttgccga
     3301 gattggtgcc agcaccaaca cccacaagct gggtcaattc gccgcccgcc atatcagagt
     3361 acaaaatggc ttcgccgcca gccgtatcgg caagagactt gaggttgaac aggtcttcat
     3421 tcgaaagacg acctgaagcc aacaccacaa agtctttctt attcgcaaca aagttctctg
     3481 ctgcggtctt gatggcgtta tcccatgaag cacgggcaag cttttcatct ttgcgaacca
     3541 tcggcttggt aaggcgttct ttgccttcgg tgtagtgata tgcaaagcgt cccttatcgc
     3601 acagccagat ttcgttcact tcttcgttct ggcgcggcat gacacgtttc actaccactt
     3661 ccccatcggc tttggcttca cgacgggtat tgagggtggt attgcatccc actgggcatt
     3721 gtgtacaaat agaagactct gccttaagtt cccacgggcg agcaccaaag cggaagtcca
     3781 ctgtggtcaa cgcacccacg gggcagatat cggttgtatt ccctgagaaa accgagtcga
     3841 aaccgggttc agagagagaa atgatttgag tgtagcgtcc gcgttcgtcg aagccgagaa
     3901 ccgcctcgcc cgcaacatcg ctctggaagc ggatacaacg tgcacattgg atgcagcgtt
     3961 cgcggtcaag atagatcaac tcaccgagcg gaaccatctt ttcgaggtgc tgtttctcgt
     4021 tgtagttgaa gcggctcttt cccggaccat gtgccatggt caaattctgc agcggacatt
     4081 ccccaccctt gtcacagatc gggcagtcca acggatggga tgtcaacagg aattcaactg
     4141 taccgcgttg accatctgtt gccttggcag attctgtaag aaccaccatg ccgtcttcca
     4201 ctttgttggt acaagcagtt tcaagcttgg gcatgaattg gatcttgggc tgtccatttt
     4261 ccatgacgaa ctgaccggtg gcacggtctc gcacagggcg accgatttcc accagacaca
     4321 tacggcacat gccaaccggc tccagcttgg gatggtggca gaagaccggg atatcgatcc
     4381 ctgctaattt tgccgcgtct gcaacgctaa gtccttctgg cgcggtaact tgttttccat
     4441 tgatcgtgag agtaacttgt ttgctcatta tctgctacgc ctttaccgct ttcttgaaat
     4501 cgtctgggaa gcgttcaata ctggtcacca cagccatagt ggagaattca cccaacgcgc
     4561 ataaacattt gccttgcatt tgcttagcaa cattcaagag tagtttaaca tcatcaccag
     4621 atcccttacc gctgtgaatg cggtccgtca ggttacgcat ccagtgattg ccttcacggc
     4681 aggtggtgca ctttccacaa gattcatgtt taaagaagtg gatggtctta ttgatgaccc
     4741 aatccatatc gactgtatcg tcgagcacaa tgaccgaggc ggaaccaagg tctgccccga
     4801 aggtacgaac cgaggcgtag tccagaggcg tgtcgaggac tttttcgtcc acaacaatta
     4861 cagaggaaga agcgcccgca ggcataatcg ccttgatggg gcgtccttct tgaatgccca
     4921 tgccatgttc gtagatcaac tggcggaagg tggtgccgaa gggcaattca taattgccgg
     4981 gcttacgcac acgacctgaa agtgaaaaga tcttcacacc ggcgctatcg gcagtgccca
     5041 tgctcttgta ccagtctgcg ccgttggcaa gaatcattgg aacattggtg aaggtctcaa
     5101 cgttattgat aatggttggc ttgccataca gacccacaga aggagggaat ggcggacgca
     5161 cacggggttg tccgcgtttg ccttcaaggg attcgagcaa agctgtttct tcgccacaga
     5221 tgtaagcacc agcgcccaag tgggtataaa tttttagaga ataatctttg ccgaaaaggt
     5281 tttcaccaag ataaccggct tcttccatct ccgcgatctt ttcatcgagc caggcagcaa
     5341 cctgccagaa ctcaccgcgc agataaatat aggcagcatt tgcgccgact gcataagcac
     5401 ccagcgccag accttccaaa aactggaagg gattcttttc catgatctcg cggtctttga
     5461 aggtacccgg ctcagactca tctgcatttg ccacaatgta gtgcggccaa gagttcttat
     5521 caatgaagga ccatttcatg cccgtcggga agcccgcgcc gccacgtccg cgcagaccgg
     5581 agttcttgac cacatcgatg acatcattcg gctgcatctt ggtgatggcg ttcttgagag
     5641 cagagaaacc accattcttc ttatagacac tcattttttc gatatcaggg atatcgcgat
     5701 ggcgtaaaag gacgtaagcc atatttactt acctcccttc aaagcttcga tcaattccat
     5761 agtacgatca atggtcatat agtcgtgata tttgatgcca tccggtccct gagtttggaa
     5821 catgggggca cggtcgcaag aggcgagaca agtcacagct tcaatggtga tgagaccatc
     5881 ttcagtggtc tcccccactt taacgccgag gttctcgcac aatttattca gaaactcatc
     5941 tgagccgcgc aatgcgcaag gtaaatcgtt gcaaacttgc atacgatatt tccctgcttt
     6001 tttatcgtga tacaaagaat agaagccgat aatggaagcg acttctgtct cggtaatatc
     6061 aagaatggag gcgatgtctt tcatcgactc tttgttgacg tagccttcct cacgctgagc
     6121 aaggtaaaga agcggcatca ccgcagagcg tttgtgctct ggcgggtatt tggcaagaat
     6181 ttgtttaact tccttcgagt atttcttttc aagactcatc ggtcaatatc tccgagaacg
     6241 atgtcaatgg aagcgaggat cgcaaccaga tcggcaacca aatgtccctt gacgatttca
     6301 gggatgacgg aaaggttgtc gaatgatggg gtgcgcatgt ggacgcggcg cggcttgggt
     6361 ccgccatcgc ctgccaacaa cacaccgagt tcaccgcgtg gggattccac tgcgctataa
     6421 atagaagcat ctggagcatg gaacccttca gtccacaact tgaagtggtg gatgagcgct
     6481 tccatgctta cgccgatctc tgaacgggga ggaggaacga acttgcggtt ctccgatcgg
     6541 acgggtccca tcggtaattt atccaacgcc tgttcaataa tgcgcaaaga ctgacggaat
     6601 tcttcgatac gcaccaagta acgagcgtag gtatcgcctt cggtcagaac gggcacatcg
     6661 aaatcatatt gttcatagcc catgtaaggg cgggctttgc gaaggtccca gttgacgcct
     6721 gaggctcgca ggttcggacc cgttaccccg taggaaagag ccgtttcttt gctcaggtaa
     6781 ccgatatcca ccatacgatc cacaaacaga ggattcttcg tcaaaagttt ttcatattca
     6841 tcaatgcgct tggggaagat cttaatgaaa tcgcgcacgg ctttttcaaa ttcaacaggc
     6901 acatcgc
//
LOCUS       DAOREF010000019         6903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5621_length_6903_cov_4.324036, whole genome shotgun sequence.
ACCESSION   DAOREF010000019 DAOREF010000000
VERSION     DAOREF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5621_length_6903_cov_4.324036"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKI33_00740"
     CDS             <1..346
                     /locus_tag="PKI33_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35551.1"
                     /translation="HAIAAAASRGNNILADHVFVEKAWVGECATLFAGTNAYLIGLYC
                     PLEVLEQREKDRKDRTLGQAKMQYDIIHKFTQYDLELDTSKLTTEECAEQVIERLKNP
                     PVAFKKLQAGHA"
     gene            377..2197
                     /gene="argS"
                     /locus_tag="PKI33_00745"
     CDS             377..2197
                     /gene="argS"
                     /locus_tag="PKI33_00745"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNM35552.1"
                     /translation="MFQKEQQLIEEKIKAYCTANDIALAELKWQPIPFSGEWGFATSF
                     FQTAANEAKLGKGTKAPVPQKAQEIAELVKAQLGSVNGISHIEAVKGYLNVYFKTSEY
                     AQRVVDEVLNGGADFGRGAAKSETVMVEYAQPNTHHSFHIGHARNTILGEVLARLVEF
                     AGYKTIRASYPGDLGLGVITVMWIYEKFYKGQEPEGVHERGQWLAKLYVEATALLEKK
                     DNETPEETAKREGYEAERRAMYRKWDAGDEAVRELWRVTREWSLEELREVLNMLDVKM
                     DVWFYESEVDEPSKAIVDELIAKDIATDERPSGGAVIVKIDEKLGLTKEKYRTNVILR
                     KDGTTLYLTKDLALAKIKFEQYHVDRSIYVVDSRQALHLQQAFAILKLWGFPQSEKCH
                     HLGYGVVNLPDGAMSARRGRVVLFKEVFDEAVKRVLSVEAEKSVEIPADEREKIAYQI
                     GMGALTYSMLSVDNNKDIVFDINEALSFDGRTGPYIQNAYVRANSILKKSKVEGQKST
                     PGSSTFDYELNKYEIELIEQISRFPQTVEQAANEYRPLVMAAYAYDLANTFHSFYHAV
                     NVMQTEDESTKKSRLSLVSAAKQTIENALRLLDIQSPDVM"
     gene            complement(2253..2612)
                     /locus_tag="PKI33_00750"
     CDS             complement(2253..2612)
                     /locus_tag="PKI33_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35553.1"
                     /translation="MKKRIIVSMFFITLLMISGCGSKSQQADVSVTPPAISVNITRGN
                     CPSMELEVGMWVAWTNVDTVSLPIKIEYPYEEAKGAGTGLSVIEPGTTFSTNFPESGI
                     FRVYCTDTDIYSTITVK"
     gene            complement(2680..4956)
                     /locus_tag="PKI33_00755"
     CDS             complement(2680..4956)
                     /locus_tag="PKI33_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HNM35554.1"
                     /translation="MSDLNPSPEFEEKVRKAMDVPNANPAFVNKLRNDLARRPVKMKP
                     IFFRPAWAGAFVLALAVLVVSVPSLAAALGRVFGYVPEVGLVETTNGLRMLAEPVSST
                     RDGVTLTITNVFAYPDHVELQYEVSGLAPENDGWQSNENNTNPTAFCGGVNVGDTHSK
                     AGDARLRLQDGTVLERDYTGKYPKNAFAMQPVYDAALPAGVTSLTFVLDCIPQARLGA
                     VPENWQINFDLIVVPEGTVIGEPVIEVNVTSEPVGTENVVTDFQNLSVKLERVVEIEQ
                     GYIFYLSVDMDHPDPLLVSIMPANVYVIDSLGQKIQLVTFDPMHPIDHPLGSAFEYQT
                     ISKPADGQLTLVVEDVIAHFDPPWINPISGPSLPQNGTFFNFDVGSNPQHGQAWKLDE
                     EFNVASYPLRVTSVRAIKYDDLGFNNDPFGSQGFDYGYQFTVETDESNKMNVALDIVN
                     QDYINSCLPVNMQSFMPEGSSMQYNILCRDGFPKGSLAVAVREVSVVGVGEWSVAWSP
                     NDALTSGFRPIAEPAIKMILEKVVPTDSSTIIYLHLNMENADPSLMYLVPQNAYVIDS
                     SGQQIHTRGNFTIEPHNHRVGDSFEFVTEAKPADGALTVVVDQVIAYYRPMYVDPPLA
                     TPEEMTFTFDAGENPQYGQVWDLNKTFTIAGYSFEIVSAQAVNYADIEKVHNYVDGSQ
                     GYDFGYQFSIEIDPPLGIAMDIIRDNCGFSDEQNVGASPILYTQLCRDGYPSGNVAVT
                     ISEISITINEDVQVGWNP"
     gene            complement(4959..5519)
                     /locus_tag="PKI33_00760"
     CDS             complement(4959..5519)
                     /locus_tag="PKI33_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM35555.1"
                     /translation="MLGLYAEEKMQNSTKDDLSALIASAQDDPAAFGRLYDRYVQPIY
                     RYLYSRLGESHAAEDVTSQTFIAAYESLHKYRERGQFSAWLFRIAQSKMNDHFRRNRR
                     EVGLEAAGELFEREDALGTLIRAEELSQVRFLIKKLNPEEQDLLRLRYVAELSFGEMA
                     DLLGKREDAVKKSVYRLLERLKGQME"
     gene            5772..>6903
                     /locus_tag="PKI33_00765"
     CDS             5772..>6903
                     /locus_tag="PKI33_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNM35556.1"
                     /translation="MPIKTIIMGAAGRDFHNFNTFFRGNKDYEVVAFTATQIPDIEGR
                     VYPKELAGDLYPSGIPIRAEEELLDLIKKYGAEQVVFSYSDVPHEYVMHKASMVNAAG
                     ADFRIMGTKNTQIKSNKPVVSISAVRTGSGKSQTTRRVAAVLQEMGYKVAAIRHPMPY
                     GNLVAQEVQRYASYDDLDEYQCTIEEREEYEPHIDSGVIIYAGVDYEKIIRKAEAEAD
                     IVLWDGGNNDFPFYLPDYQIVVADPHRPGHEFTYYPGETNVRMADCFVINKVDTADAE
                     AVIKVRESLRKLNPTAVQIEGASPLFVDDPDAIFGKRVLVVEDGPTLTHGEMAYGAGY
                     VAARRFGAKEIVDPRPFAVGSIAKTFEKYPKTGAILPAMGYGE"
BASE COUNT         1910 a         1714 c         1632 g         1647 t
ORIGIN      
        1 tcacgccata gccgctgccg cttcgcgtgg aaacaacatc cttgccgacc acgttttcgt
       61 tgaaaaagcc tgggtgggcg aatgcgccac gctctttgca gggacgaacg cctatctcat
      121 cggcttgtat tgtccgctgg aagtgttgga acaacgtgag aaagaccgca aagaccgcac
      181 ccttggtcaa gccaagatgc aatacgatat cattcacaaa ttcacccaat acgacctcga
      241 gttggatacg tccaaactga ccactgaaga atgtgcagag caggtcattg agcgattgaa
      301 aaacccacca gttgctttca agaaattaca ggcaggtcac gcgtaaagcg cgaccctaca
      361 gaaggatcgc ttcgctatgt ttcaaaaaga acaacaactc attgaagaaa agatcaaagc
      421 ctactgcacc gccaatgaca ttgctttggc agaattgaag tggcagccca tccccttctc
      481 aggtgaatgg ggttttgcta cttccttctt ccagactgct gccaacgaag caaaactcgg
      541 caaagggaca aaggctccgg tgccgcaaaa ggctcaagaa atcgccgagc tggtcaaggc
      601 gcagttggga agcgtgaatg ggattagtca catcgaagcg gtgaaaggct atctcaacgt
      661 ctacttcaaa acatctgagt atgcccagcg tgtggtggac gaagtgctga atggcggcgc
      721 ggactttggt cgcggtgcgg caaagtctga aacggtcatg gtcgaatatg ctcagccaaa
      781 cacacatcac tccttccaca ttggacatgc gcgcaacacg attcttggcg aagttctcgc
      841 aaggctggtg gaattcgcgg gctataaaac catccgcgcc tcataccctg gtgacctggg
      901 actcggcgta atcacggtga tgtggatcta cgaaaaattc tacaaaggtc aggaacctga
      961 aggcgtgcat gagcgcggac aatggcttgc aaaactttat gtagaagcca ctgccttgct
     1021 cgaaaagaaa gataacgaga cacccgaaga aactgcaaaa cgtgaaggct acgaagccga
     1081 acggcgcgcg atgtaccgca aatgggacgc gggcgatgaa gccgtgcgcg agttgtggcg
     1141 cgtaacacgc gagtggagtt tggaagaact gcgtgaagta ctgaacatgc ttgacgtgaa
     1201 aatggatgtt tggttctacg aaagcgaagt ggatgagcca tccaaagcca ttgtggatga
     1261 gttgatcgcg aaagatattg ccaccgatga acgcccgagc ggcggcgcag tcatcgtaaa
     1321 aattgacgaa aagcttgggc tgacaaaaga aaagtaccgc acgaatgtaa tcctgcgaaa
     1381 agacggcaca accttgtact tgaccaagga ccttgccttg gccaagatca agttcgagca
     1441 atatcatgtt gatcgttcta tctatgtggt tgattcacgg caggcacttc atctacagca
     1501 agccttcgcg atcttgaagt tatgggggtt cccgcaatca gagaaatgcc atcacctagg
     1561 ttatggggtt gtgaatcttc ccgatggcgc catgtctgca cggcgcggac gtgtggtgct
     1621 gtttaaggaa gtattcgacg aagccgtgaa gcgcgtgctt tcggtggaag ccgaaaagag
     1681 cgtggagatc cccgccgatg aacgcgagaa gatcgcctat cagatcggca tgggcgcatt
     1741 gacctattcc atgctttcgg tagacaacaa taaagatatt gtgttcgaca tcaacgaggc
     1801 attgtcgttt gatggacgga cggggccgta tattcagaat gcgtatgtgc gggcgaactc
     1861 gattttgaag aagtcgaagg tcgaaggtca aaagtcaacc cctgggtctt caacattcga
     1921 ctatgaatta aacaagtatg aaattgagtt gatcgagcaa atctcgcgct tcccgcagac
     1981 tgtggagcag gcagccaatg agtaccgtcc gcttgtgatg gcagcgtatg cctacgatct
     2041 agcgaatacg ttccattctt tttatcatgc ggtgaatgtg atgcagacag aagacgaatc
     2101 tacaaaaaaa tcacgtctga gtcttgtatc tgccgcgaag cagacgatag aaaatgcgtt
     2161 gaggctgttg gatattcaat cgccggatgt aatgtagaat aaagaaagca gaagatgaaa
     2221 acaagctggg cttgcggccc aacttgtttt ttttatttca cagtgattgt tgaataaata
     2281 tctgtgtcgg tgcagtacac acgaaaaatc ccagattcag gaaagttcgt tgagaaagtc
     2341 gtccccggct caatcacaga caaacctgta ccagcgcctt ttgcttcttc atacgggtat
     2401 tcgatcttga taggcaggga caccgtatcc acattcgtcc acgctaccca cattcccacc
     2461 tctagttcca tggaagggca atttccccgt gtaatattca cactaatggc aggcggggtg
     2521 acagatacat ccgcctgttg ggatttactt ccacaaccag aaatcatcaa caatgtaata
     2581 aaaaacatgg aaacaataat tcgttttttc ataaattctc cttttataaa atcttaaagc
     2641 aggtttaccc aaacagccaa cctgctttag tactattttc tatggattcc accctacttg
     2701 cacatcttcg tttatcgtaa tggatatttc actgatcgta acagccacat tccccgacgg
     2761 gtatccatct cggcacagtt gggtataaag tatgggcgaa gcgccaacat tttgctcatc
     2821 agagaagccg cagttatccc tgataatatc cattgcaatg ccaagcggtg ggtctatttc
     2881 gatggaaaat tgataaccaa agtcgtagcc ttgtgagcca tccacatagt tatggacctt
     2941 ctctatatcg gcgtaattca cagcctgtgc agagacaatc tcaaatgaat accctgcaat
     3001 tgtgaatgtc ttattcaagt cccacacctg accatattgc ggattttcgc cagcgtcaaa
     3061 ggtaaaggtc atttcttcgg gggttgccag cggcgggtca acatacatgg gtctatagta
     3121 tgcaatgact tgatccacga ccacagtcaa cgcgccatcg gcaggcttgg cttcagtcac
     3181 aaattcaaat gaatcgccaa ctctgtgatt atgcggctca atggtgaaat ttccgcgcgt
     3241 atgaatttgc tgccccgaag agtcgatcac ataagcattt tgcggcacaa ggtacatcag
     3301 ggacgggtcg gcattttcca tgttgagatg gaggtaaata atggtgcttg agtcagtagg
     3361 tacaaccttt tccaatatca tttttatggc tggctcagca atagggcgga aaccgcttgt
     3421 gagagcgtca ttcggcgacc aagccacact ccactcacca acacccacaa cagaaacttc
     3481 acgcactgcg actgcaagag atcccttagg aaaaccatcg cggcataata tgttgtactg
     3541 catggatgaa ccttccggca tgaatgactg catattcacc ggcaaacatg aatttatgta
     3601 atcttggtta acaatatcca acgcaacatt catcttattg gattcatctg tttcaacagt
     3661 aaattgatag ccataatcaa acccctgtga accgaacgga tcattattaa aacccaggtc
     3721 atcatactta atggcgcgaa cagatgtcac tcgcaaagga taactggcaa cattaaattc
     3781 ttcatctaac ttccaggctt gcccatgttg cggattgctt ccaacatcga agttaaaaaa
     3841 cgttccattt tgaggcagag atggaccact aattgggtta atccaaggcg ggtcaaagtg
     3901 agcaatgaca tcttccacaa ccaaggtcag ttgaccatca gcaggtttgg atatagtctg
     3961 atattcgaat gcactgccaa gcggatgatc aatcggatgc atgggatcaa aagtcaccaa
     4021 ctgtattttc tgtcctaaag agtcgattac atacacgttg gcaggcatta tcgatactaa
     4081 taatggatca ggatgatcca tatccacact caaatagaaa atatatcctt gctcaatttc
     4141 cacaactcgt tccaacttta cagataagtt ttgaaaatca gtaaccacat tttctgttcc
     4201 gacaggttct gaagtcacat tgacctcaat aaccggctcg ccgattacag taccttcagg
     4261 aacaacaatc aaatcaaaat taatctgcca gttttccggc acagcgccaa ggcgggcttg
     4321 cggaatgcaa tcgagcacga aggtcagcga agtgactccc gctggaagtg cagcatcata
     4381 aacaggctgc atcgcaaacg catttttcgg atatttgccc gtgtaatcgc gttcaagcac
     4441 agtgccatct tgcaggcgta aacgcgcatc acccgctttg ctgtgcgtat ctccaacgtt
     4501 cacaccgccg caaaatgcgg tcggatttgt attgttttca tttgattgcc agccatcatt
     4561 ttcaggcgca agtccgctca cctcatattg caattcaaca tggtcggggt aggcaaaaac
     4621 atttgtgatg gttagagtaa ctccgtcacg agttgacgaa actggttctg ccaacatacg
     4681 caatccgttt gttgtttcaa ccaaaccaac ttcgggcaca tagccgaaca cacgtcccaa
     4741 tgcggcggca agactcggta cgctcactac aaggactgcc agagccagca caaatgcccc
     4801 agcccaggcg ggtctaaaaa aaatcggttt catttttact ggtctccgtg ccagatcatt
     4861 tcgtaattta ttgacaaacg cagggttcgc attcggcaca tccattgcct tgcgtacctt
     4921 ctcttcaaat tcaggggagg gattcaaatc actcatggtt actccatctg tccctttaac
     4981 cgttccagca aacggtacac tgatttcttc acggcgtcct cccgttttcc aagcaagtct
     5041 gccatctcgc caaaagacaa ctcagccaca taacgcaagc gcagcaaatc ctgttcttca
     5101 ggatttaatt ttttaatgag gaagcggacc tgactcaact cttcagcacg gatcaacgtc
     5161 ccaagcgcat cctcccgctc gaacagttcc cccgccgcct ccaacccgac ctcgcgcctg
     5221 tttctgcgga agtggtcatt catcttgctt tgagcgattc ggaacagcca cgccgagaat
     5281 tgtccacgtt cgcgatactt atgcaaagac tcatatgccg caataaacgt ctgcgatgtg
     5341 acatcctctg ccgcgtgaga ctccccaagt cggctgtata aatagcgata aattggctgg
     5401 acgtaacggt catatagcct gccaaacgca gcagggtcat cctgtgcgga tgcaatcaat
     5461 gcagacagat catctttggt tgaattttgc atcttttctt ctgcatataa tcctaacatg
     5521 caaatccttt ctctcatgag gcttcttcat ttatgaatgc attccaaggg ttaaacgggt
     5581 agaaaacgaa aaagggacaa atttctgtca tgccaaaatc aggctaaacg ttacttcacc
     5641 agagagacaa attcccttag attagacatt aactatcgta aatgtcatcg tgagtaaaaa
     5701 cgagcaggct caaatcccag aacgggccag ttcggacact gtcctcaaaa tgacatgcac
     5761 aggagtttcc tatgccgatc aaaaccatca tcatgggcgc cgcagggcgc gattttcaca
     5821 acttcaatac atttttcaga gggaataagg attacgaagt tgtagctttt actgccaccc
     5881 agatccccga cattgaagga cgcgtctatc cgaaagagtt ggctggtgac ttgtacccga
     5941 gcggaatccc catccgagcc gaggaagaac tgctcgacct catcaaaaag tacggcgcgg
     6001 aacaggtcgt cttctcctac agcgatgtgc cgcatgaata tgtgatgcac aaagccagca
     6061 tggtcaacgc cgccggtgca gacttccgca ttatgggtac gaagaatacc caaattaagt
     6121 cgaacaagcc ggtcgtgtcg atcagtgccg ttcggacggg atcaggcaaa agtcagacga
     6181 cgaggcgcgt ggctgccgtc ttgcaagaga tgggctacaa agtcgcagcg atccgtcacc
     6241 ccatgccgta tggaaatctc gtcgcgcagg aagtgcagcg ctatgcttca tacgatgacc
     6301 tcgatgaata tcaatgcacc atcgaagagc gcgaagaata tgaaccgcac atcgacagtg
     6361 gtgtgatcat ttatgccggt gtcgattacg aaaagatcat ccgcaaagcc gaagccgaag
     6421 cggatattgt gttgtgggat ggcggcaata acgacttccc gttctattta cccgattatc
     6481 agatcgtggt cgctgaccca catcgccctg ggcatgaatt cacgtattac cccggcgaaa
     6541 cgaatgtccg catggcagat tgttttgtga tcaacaaagt ggataccgcc gatgcagaag
     6601 ccgtcatcaa agtgcgcgag agtttgcgca agttgaaccc aactgcggtg cagatcgaag
     6661 gcgcctctcc cctcttcgta gatgatcccg atgccatctt tggcaagcgc gttctggtcg
     6721 tggaagatgg gccaaccctc acccatggcg aaatggcgta tggcgctggt tacgttgccg
     6781 cccgccgctt cggcgcgaaa gagatcgtgg accctcgtcc gttcgcagtt ggttcaattg
     6841 ccaaaacctt cgagaagtat ccgaagacag gtgcgatttt gcctgcgatg ggctatggcg
     6901 aaa
//
LOCUS       DAOREF010000020         6855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5666_length_6855_cov_4.622794, whole genome shotgun sequence.
ACCESSION   DAOREF010000020 DAOREF010000000
VERSION     DAOREF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5666_length_6855_cov_4.622794"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKI33_00770"
     CDS             <1..585
                     /locus_tag="PKI33_00770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNM35557.1"
                     /translation="PLRRPRRECHEGEAPDERPRRDTTMESLGKLKAAFRDGGTVTAG
                     NSSGLNDGASALLIMSEEKAKALNLKPLARIVTSASAGVPPRVMGIGPVPATKKAVER
                     AGLKMQDIGLMELNEAFAVQSLAVIEDLGIDPELVNVNGGAIAIGHPLGCSGARLVTT
                     LVHEMKNRGNVKYAVATLCVGVGQGEATVIEYLG"
     gene            610..1803
                     /locus_tag="PKI33_00775"
     CDS             610..1803
                     /locus_tag="PKI33_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35558.1"
                     /translation="MKRFLSFLFILTALVSACAPAVATTAAPEASEPAATGTEEPQAL
                     SQAVLNTPLPAVAEAPIIESPAILNIQMLDEVNGWALTEEKVVRTNDGGVTWYDVTPK
                     NLKDAGYLVNTDFLDENTAWVQFPDMNKYPNGGTLYHTTNGGLTWESFSTPFSGGYIQ
                     FIDANNGWMLSDLGVGAGSMAVSVFQTSDAGKTWNRTYTNDPNLADASDTLPLGGIKN
                     LILPLNAKTAWIGGVVYAPGETYLFRSDDNGKTWVNINLILPQEAAGSELSVIGMVFL
                     SPKTGLLALRVATETPKTIVYVTQDGGNTWTQSEVSFEGYGLLETPSANEMVFYVNDQ
                     FYVTKDAGVTVQEIIPDVKFGDAIMDMSFANAQTGWIVAADASGNYTLYKTTDSGSTW
                     TVLSQ"
     gene            1915..2319
                     /locus_tag="PKI33_00780"
     CDS             1915..2319
                     /locus_tag="PKI33_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNM35559.1"
                     /translation="MNNLVRDWMSSPVVVVDPDSSVSHAMTLMRRRNIHSVVVDVSEK
                     NPSYGIVTTTDVRDKIVVAGRNPAETTVREIMSGPIVTGRAEWTLAECSRLMQERKIR
                     HLPIADEHGTLIGLISATDIFMAVEEQGWIEG"
     gene            complement(2369..2800)
                     /locus_tag="PKI33_00785"
     CDS             complement(2369..2800)
                     /locus_tag="PKI33_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNM35560.1"
                     /translation="MTLPNKLQEIVDDFASMSREEKLETLIAYAESMPELPERFHAER
                     EKMEAVPECMTPVFIAAEKDSNNGIVFHLDIPKQSPTVRGLASILTNGLNGCSLAEII
                     SVPAEFYLPMNLQEAVSQQRINGFMGVLAHMKQVAVRVGSG"
     gene            complement(2864..3703)
                     /locus_tag="PKI33_00790"
     CDS             complement(2864..3703)
                     /locus_tag="PKI33_00790"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNM35561.1"
                     /translation="MSYAHPEYLVETDWVAEHLNDPNIRIIESDEDPLLYAIGHIPGA
                     AQVDWFSTLQHPLRRDFLTKEQFEQVASKLGITNDTTVIFYGDKSNWFACYALWLFQY
                     YGHQNVKIVNGGRAKWEKEGRPLVKEVPTFTATTYKAKEADKSIRAFRDDVFKQVDSK
                     KPLVDVRSPKEYTGELLHMPNYPQEGATRGGHIPGAVSIPWAQAVNEADSTFKTAEEL
                     KALYEGKNIKSDGEIIAYCRIGERSSLTWFVLKYLLGYPTVKNYDGSWTEWGNLVDAP
                     IEK"
     gene            3992..4615
                     /locus_tag="PKI33_00795"
     CDS             3992..4615
                     /locus_tag="PKI33_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNM35562.1"
                     /translation="MARQKYYVVWKGRKTGIFTSWAECEKQVKGVVGAQFKAFESEAE
                     ADAAYLAKYEDYIGKVSTSGRWREASIKPLLPSVCVDAACSGAPGTLEYRGVNTETGE
                     EIFHSGPYADGTNNVGEFLAIVHALTWQEKHNIHIPIYSDSENAISWVHTGKCRTKLA
                     HTSKNSILFAIIKSAENWLAENELPEGKIIKWDTNVWGENPADFGRK"
     gene            4681..6261
                     /locus_tag="PKI33_00800"
     CDS             4681..6261
                     /locus_tag="PKI33_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNM35563.1"
                     /translation="MRFFGLSWSLGRLRGVDIRFHFSMLFSIPIAYYLFDPIDLRGVV
                     EALLWLVGFTIFILLHEVGHAFAAQMVGVEVKSIVVWLLGGLTNLAYKPEKPVHNFFV
                     YAAGPLMNMLLAFLCVAAYILTAMLFLPFSKDPQTYIWIQTFQNLFFSLAIVNLILAL
                     FNLIPVYPLDGGNIFHAAMEWLFGKTNADRITLVVGIPFLLVLIVFGVISRDYVLLAF
                     CVLIALSISTLNHSLLRNINLGITYLFKRAGYYYLKGDYERAAQLYTAEIEKQPDDIN
                     NYLARAGCYLAMAQRERALADVDRVLKLHPNHFLALELRGEILMLNKDYDAAFAHFKQ
                     VQHINANWAVPYFDLASLYMERGELQPAIENFNKAVVLQPRMPLFYIIRSLAHFKAGD
                     LASAHHDQDVAVGLSPEEALVMVDVNLIVYEDHWDWAKDYYERILTKNPRNAFALQGF
                     AEACLVNQKWDEAVNFFSRAIEQNPREARLYVGRGKAYVELEKLQEAKVDFEKAVASS
                     EKLHLKRQAENFLRTLDI"
     gene            6276..6845
                     /locus_tag="PKI33_00805"
     CDS             6276..6845
                     /locus_tag="PKI33_00805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNM35564.1"
                     /translation="MKTALLVIDIQKDYFPGGKFPLVEPLAAAEKAYGLLQCFRENNE
                     KHVHIQHISLKPDATFFVRGTEGSDIHDAVAHFEGEPIVYKHYPNSFRETTLFDMLKG
                     WGIERVVITGMMTHMCVDATVRAAADFGFQVWVAEDACATRDLEHNGTVVPADHVHRA
                     FLAAFKSYGQVMMSEQVIARLAAEKASAR"
BASE COUNT         1713 a         1487 c         1787 g         1868 t
ORIGIN      
        1 ccgctccgac gacctcgacg tgaatgccac gaaggagaag caccagacga acgtccgcgc
       61 cgcgacacga cgatggaaag ccttggtaag ctcaaagccg catttcgcga cgggggaact
      121 gtgaccgcag gaaattcgtc tggattaaat gatggcgcat cggcgctgtt gatcatgagt
      181 gaagaaaagg cgaaagctct caacctcaag ccgcttgccc gcattgtgac ttctgcttcg
      241 gcaggtgtgc ctccgcgtgt gatgggcatt ggacctgtcc ctgccacgaa gaaagcagta
      301 gagcgcgccg gtctaaaaat gcaagacatt ggactgatgg aattgaacga agcctttgct
      361 gttcaatctc tggcggtgat cgaagacttg ggcatagacc ccgaactggt caacgtcaac
      421 ggcggcgcga ttgccattgg gcatccgctt gggtgttcag gtgcgcgcct cgtgaccacg
      481 ctggtgcatg agatgaaaaa tcgcggcaat gtcaaatacg ctgtggcgac tctttgcgtg
      541 ggtgtggggc aaggcgaagc gaccgtgatt gagtatcttg gataatttat tgatagccaa
      601 aggagaacca tgaaacgatt tctctctttc ttatttattc ttacggcact ggtgagtgct
      661 tgtgctcctg ccgtagcaac aacggcggct ccggaggctt cagaacctgc ggcgacaggc
      721 acagaagaac ctcaagcttt atcccaagca gtgctcaata cacctttgcc cgctgttgcc
      781 gaagcgccca tcattgagtc tcctgctatt cttaacatcc aaatgttgga tgaagtgaac
      841 ggatgggcac tcacggaaga aaaagttgtc cgcaccaacg atggcggcgt gacctggtat
      901 gacgtcaccc cgaaaaactt gaaggatgcc ggctatctcg tcaacaccga ttttctggat
      961 gagaatacgg cttgggtgca attccctgat atgaacaaat atcccaatgg tggtacgctc
     1021 tatcacacca ccaatggcgg cttaacatgg gaaagctttt ctacgccatt cagcggcggt
     1081 tatattcaat ttattgatgc aaacaacgga tggatgctat ctgaccttgg tgttggtgct
     1141 ggttctatgg cggtttctgt ttttcagacc agtgatgctg gcaaaacttg gaatcgcact
     1201 tacaccaatg accccaatct tgctgatgca agtgatacgc ttccgttggg tggtattaag
     1261 aatctcatct tgccgctcaa tgcaaaaaca gcgtggatcg gtggcgtggt ctatgccccc
     1321 ggtgaaacct atcttttccg cagtgacgat aatggcaaaa cctgggtcaa tatcaacctt
     1381 atccttccgc aagaagcagc aggcagtgaa ctgagcgtga tcggaatggt cttcctctcg
     1441 cccaagacag gtctgcttgc gttgcgggtt gccacagaaa cacccaagac cattgtatac
     1501 gtcacccaag atggcggtaa cacgtggacg caatctgaag tttcgtttga aggttatgga
     1561 ttattggaaa cgccgtcagc gaatgaaatg gtcttttatg tcaatgacca gttctacgtc
     1621 acgaaagatg cgggtgttac tgtccaagaa attattcccg atgtgaagtt cggcgacgcc
     1681 atcatggata tgagttttgc gaatgctcaa actggttgga tcgttgctgc tgatgcctct
     1741 ggaaactata ccttgtataa gacaacggat agcggctcaa catggacggt tctaagtcag
     1801 taaacgcttc tttccccgcc gacgtttgat tttgttgctc gaccctttgg ttacgctgtg
     1861 agtaacctga tagttacacc catactgtaa aatatctata aaggagagag aattatgaat
     1921 aacttagttc gtgattggat gtccagcccg gttgttgttg tagaccctga ttcgagcgtt
     1981 tcacatgcca tgacgctcat gcgtcgtcgc aatattcata gcgtggttgt ggatgttagc
     2041 gagaagaacc cttcatatgg cattgtaaca acaacagatg tgcgcgataa gatcgtggtt
     2101 gccgggcgta acccagcaga gaccactgtg cgcgagatta tgtctggtcc cattgttacc
     2161 gggcgtgccg aatggacgct ggcagaatgc tctcgactga tgcaagaaag aaaaattcgc
     2221 cacttgccaa ttgcagatga acatggcacc ttgatcggct tgatctctgc caccgatatt
     2281 tttatggcag tggaagaaca aggttggatc gaaggctaga aaataaaaac agtcccgtaa
     2341 agggactgtt ttttgttact tgtaaaattt accctgaccc aacccgtaca gcaacttgtt
     2401 tcatatgtgc cagcacgccc ataaaaccat taatgcgttg ttgggatacg gcttcttgta
     2461 gattcatagg caggtaaaac tcggcaggca ccgaaatgat ctctgcaagc gagcagccat
     2521 ttaaaccgtt ggtcagaatc gatgccagtc cgcgcacggt gggagattgt ttcggaatat
     2581 ccaagtggaa gacgatgccg ttgttgctgt ctttctcggc ggcaatgaaa acgggcgtca
     2641 tgcattctgg caccgcttcc attttttcgc gttctgcatg gaagcgctct ggcaactctg
     2701 gcatggattc ggcgtatgcg atcagcgtct ccagcttttc ttcgcggctc atgctggcaa
     2761 agtcatctac aatttcttga agtttattgg gtagggtcat aatgttccgg gtaagggcga
     2821 gcttatacca ctctgtgata taaacctcgc ctctacgata ctattatttt tcaattggtg
     2881 catctacgag gttgccccat tcagtccagg aaccgtcgta gttcttgacc gtgggataac
     2941 ccagcaaata ctttaatacg aaccaagtca acgaagagcg ttccccaata cgacaatagg
     3001 cgatgatttc accatcggat ttgatgtttt tgccttcgta aagcgccttc aattcttcag
     3061 cggtcttaaa ggtggaatcg gcttcgttga cagcctgtgc ccacggaatg cttaccgctc
     3121 cggggatgtg tccgccgcgg gtggcgcctt cttgtgggta gttgggcatg tgcaataatt
     3181 cgcccgtata ttccttcggc gagcggacgt ctaccaaggg tttctttgaa tcaacctgct
     3241 tgaagacatc gtcgcggaag gcgcgaatgg acttatcggc ttctttggct ttgtaggtag
     3301 ttgcagtgaa tgtgggaact tccttcacga gtgggcgacc ttctttttcc catttcgcac
     3361 gtccgccgtt tacgatcttc acgttctggt gaccataata ttggaagagc cagagcgcat
     3421 agcaggcaaa ccaattcgac ttatcgccat aaaagatgac agttgtatca ttggtgatgc
     3481 caagcttgga tgcaacctgt tcaaattgtt ctttggtgag aaagtcgcgg cgcagtgggt
     3541 gttgaagcgt gctgaaccag tctacttggg cggcgccggg aatgtgacct atggcataca
     3601 gcaagggatc ttcgtccgat tcgataatac gaatgttggg gtcatttaga tgttcagcga
     3661 cccagtctgt ttctacaagg tattcggggt gagcgtacga catgataaag tttccttttc
     3721 taagcaagaa gtatatgcaa aataaaaagc agccaaccga acaaaaagga cagcgaacca
     3781 tcctgcgcgg acatcatttc acgcgggagc cgttccactg ccttgagagt tgactgctta
     3841 cttgggcgga tattgtctca ggcggtggtt acgaaccccg ccacagacag gagcaaaatt
     3901 gcgaagtact tttcattgag cgcgtatttt acgtgattga tgtcgtttgt caagacaaat
     3961 attcaaaact gaattgatgt aaaattgacg catggcgcgt cagaaatatt acgtcgtatg
     4021 gaaaggacgc aagaccggca tcttcacgtc ttgggcggaa tgtgaaaaac aggtgaaggg
     4081 cgtggtgggt gcgcaattca aagcgtttga aagcgaagcc gaagcagacg cagcctattt
     4141 agccaagtac gaagactata ttgggaaggt atctaccagt ggacgctggc gtgaggcaag
     4201 catcaaacct ttgttgccgt ctgtttgtgt ggatgccgca tgcagtgggg cgcccggcac
     4261 gttggaatat cgcggtgtaa atactgaaac aggcgaagag atctttcatt cgggtccata
     4321 tgccgatggg acgaataatg ttggggagtt tttggcgatt gtccatgcat tgacatggca
     4381 ggaaaaacat aatatccata tcccaatcta ttcagactcg gaaaatgcaa tttcatgggt
     4441 tcataccggt aaatgccgta caaagttagc acatacctca aagaattcta ttttatttgc
     4501 catcatcaaa agtgcggaga actggctggc agaaaatgaa ttgccagaag gcaagatcat
     4561 taagtgggat accaatgtat ggggcgaaaa cccggcagat tttggtcgaa agtgaaaagt
     4621 gagaatccat tcaatggtga gaatcaattt cagttcccgt atgaggcggg ggtttaagtc
     4681 atgagatttt ttggtttatc ttggtcgctg ggtcgcctgc gtggagtgga tattcgtttc
     4741 catttttcga tgctgtttag cattccgatt gcttattatc tttttgaccc gattgacctg
     4801 cgtggtgtgg ttgaagcttt attgtggttg gttggcttta cgatatttat tcttttgcat
     4861 gaagtggggc atgcgtttgc cgcgcaaatg gtaggcgtgg aagtgaaaag cattgtggtc
     4921 tggctcttgg gcgggttgac aaaccttgcg tataaaccag agaagccagt acataatttc
     4981 tttgtttatg cagccggacc tttgatgaat atgctgttgg cgtttttgtg tgtggcggca
     5041 tatattctta cagccatgct ttttttgccg ttttcgaaag acccgcaaac ttatatttgg
     5101 atacaaactt ttcagaacct tttcttttcg cttgcaattg tcaatttgat tttggcattg
     5161 ttcaacctta tcccggttta cccgttggat ggcggtaata tttttcacgc tgccatggaa
     5221 tggttatttg gtaaaaccaa tgcagaccgc atcactcttg tagtgggtat tccatttttg
     5281 ctggtattga ttgtcttcgg cgttatctct cgtgattatg tcttgcttgc tttttgtgtg
     5341 ctgatcgcac tttcgatcag taccttgaat cattccttgc tcaggaatat caatcttgga
     5401 attacctatc tctttaagcg agctggctat tattatttga agggtgatta cgaacgtgcc
     5461 gcgcaacttt atacagctga gatcgaaaaa cagcctgacg atattaataa ttatttggca
     5521 agagctgggt gttatcttgc catggctcag cgtgagcgag cacttgccga tgtagaccgt
     5581 gtgctcaaat tacatccgaa tcattttctc gcacttgaat tgcgtggtga gattctgatg
     5641 ctcaataaag attacgatgc tgcgtttgca catttcaagc aggtgcaaca cataaatgcg
     5701 aattgggcag ttccttactt tgaccttgct tcactctata tggaacgtgg tgagttacag
     5761 cctgcaattg agaactttaa taaggctgtt gtcttgcagc cgcgcatgcc tttgttctat
     5821 atcatccgtt cactggcgca ttttaaggcg ggcgacctcg cgtctgcgca tcatgaccaa
     5881 gatgtggcgg tggggctttc gccagaagaa gctttagtga tggtggatgt taacctgatt
     5941 gtgtatgaag accattggga ttgggcgaag gattactatg agcgtatctt gacaaaaaat
     6001 ccgcggaatg cctttgcgct tcagggcttc gccgaagctt gcctcgtcaa ccaaaaatgg
     6061 gatgaggctg tgaacttttt ctcgcgtgca atagagcaga atccacgaga ggcacggctt
     6121 tatgtggggc ggggaaaagc atatgtagaa ttagaaaaac tccaagaggc aaaagtagat
     6181 tttgaaaaag ctgttgcgtc gtcggaaaaa ttgcatttga aaagacaggc agaaaatttc
     6241 ttaagaacct tggacatata ggaaaaggat gtgttatgaa aacagcttta cttgtgattg
     6301 atatacaaaa agattatttc cccggcggga aattcccgct cgtagaaccg cttgcggctg
     6361 cagaaaaagc atatggactg ctacaatgct ttcgcgaaaa caacgaaaag catgttcata
     6421 ttcaacatat ttcgctcaag cccgatgcga cattctttgt ccgtggaaca gaaggctcag
     6481 atattcatga tgcggtggcg cattttgaag gcgaacctat cgtctacaaa cattacccca
     6541 attcgttccg ggagacgact ttgttcgata tgcttaaggg atggggaata gagcgtgtag
     6601 tcatcacagg gatgatgacc catatgtgcg tggatgcaac tgtccgtgcg gcggctgatt
     6661 ttgggtttca ggtttgggtg gcagaagatg cttgtgccac gcgcgatttg gaacataatg
     6721 gcactgttgt ccccgcggac catgtccata gggcgttttt ggcggcgttc aagtcgtatg
     6781 ggcaggtgat gatgtcggag caggtgattg cgagattggc tgcagagaaa gccagtgcta
     6841 ggtaagtaga gtcaa
//
LOCUS       DAOREF010000021         6827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5707_length_6827_cov_4.381276, whole genome shotgun sequence.
ACCESSION   DAOREF010000021 DAOREF010000000
VERSION     DAOREF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5707_length_6827_cov_4.381276"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /gene="rnpA"
                     /locus_tag="PKI33_00810"
     CDS             <1..337
                     /gene="rnpA"
                     /locus_tag="PKI33_00810"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNM35565.1"
                     /translation="KRVRRTGKSYAHPLVVLVTQASETKEHLKVGIAAGKTTGTAVSR
                     NRAKRLLREAMRDLIPSITLRLAQENASGWDLILIARPGLVTATLADTRSALTNLLKR
                     AKLLPVDES"
     gene            327..638
                     /gene="yidD"
                     /locus_tag="PKI33_00815"
     CDS             327..638
                     /gene="yidD"
                     /locus_tag="PKI33_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNM35566.1"
                     /translation="MNPDTEEQHEHFVLHDYSHEPRLRDMPRTLGNIPRIVVLSIIRL
                     YQMAISPGLPANTCRFYPSCSHYGYQAVYKHGAFKGSFMAAWRVLRCNPFNPGGYDPV
                     P"
     gene            702..1775
                     /locus_tag="PKI33_00820"
     CDS             702..1775
                     /locus_tag="PKI33_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNM35567.1"
                     /translation="MKKINLILLLVLGAVLLSACTGGRASIVNTWAGLAADGERAYLS
                     SGSHVYAVDVQTGKEVWRYPAETDSNIIFYANPVLTNDGQLLVGSEGNNHELVSINPE
                     TGKDNWAKPFSVAKGKWVASPLVFNDIVYAPNSDGFLYILDLNGETSADPIELGGSLW
                     STPVTDGTYIYVTSLDHHLHIVDPASNSIVSTIDLGGASPSSPVVGEGGVYVGSFASN
                     IEFVTSNGDHEVLATATNWIWGTPALDNGTLYYADLDGVLYSLDVNSGKQNWTNVKPD
                     GPVIANPVVVGDAIYFVTEAGTFLALDRDAKTIWEKEVGGKIYTTPAVSGDMVLVAPY
                     KAEFALAAYDAEGKQAWTFTPEK"
     gene            1788..2606
                     /locus_tag="PKI33_00825"
     CDS             1788..2606
                     /locus_tag="PKI33_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNM35568.1"
                     /translation="MWESFIIQPMVNLLLWIYDILGHGPQMFGLAIILFTIVIRLITW
                     PLNASQVKGAQAMQELNSDKEWQDIQKKYAKDREKLAQEQMRIYKERGINPFASCLPT
                     LIQFPIIIGLYQSIIRAMAATPLDMLNLARTIYPFQHVSNIIPLNSTFLWMNLGRPEG
                     IPLPFDVSFLPNGFPLLALVVAITTYVQTKLTMPTTPPNPNDQASAQTAAMSSSMSIY
                     MPLMLGWFALTFASGISIYFITSNTLGIIQYAVTGRANWSNLLPGGNKSTPAKK"
     gene            2616..3479
                     /gene="jag"
                     /locus_tag="PKI33_00830"
     CDS             2616..3479
                     /gene="jag"
                     /locus_tag="PKI33_00830"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNM35569.1"
                     /translation="MSERTTLEIIAPTVEEAIQQGLDQLGLTADAVSVEVLDSGSKGL
                     FGLGGRQVRVRISVNPPPMPEVPAARPEPKPAPAKKAESKPKAKEPKEPKPRAEKKQA
                     EPKTEAKPEPKPEPKPQKPAPQRSTINVENDAVLNTAEEVVSKLIYHMGMTAQVSAHY
                     DESDSADNRTIQVDVRGEDLSALIGRRAETLASFQHVASLIVGKQTQQWVQVIIDVEG
                     YRARREKQIRQLANRMADQVTKTGRKVTMEPMPSSERRVVHIELRGHPAVKTESTGEE
                     PYRKVVILPKE"
     gene            3590..4432
                     /locus_tag="PKI33_00835"
     CDS             3590..4432
                     /locus_tag="PKI33_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNM35570.1"
                     /translation="MSDLRPVDYLLIGHVAVDLTPSGKQLGGTVSYAALTAKAMGLRV
                     GIVTSAGEDAPLSLLDGVQIFNLPTEQSTIFENVKTEHGRKQTLHHQATKISFEHVPQ
                     IWRDAAIVHLAPIAQEVDPVLVGQFPASWVGVTPQGWMRGWDEKGSVFATAWENSEQV
                     LGQVGGVVMSLEDINHDLELGEWLAHHARLLCLTEGEEGAVLHWHGDRRRYRPIKVEE
                     VDATGAGDIFAAGFFSRLHQTKDPWEAARFATHLAAYSVTRVGLQGIPTHDEIEHCKM
                     EVLS"
     gene            4429..5199
                     /locus_tag="PKI33_00840"
     CDS             4429..5199
                     /locus_tag="PKI33_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNM35571.1"
                     /translation="MTKIYTLVNQKGGVGKTTSTINLAAYLAKLGQRVLVVDLDPQAN
                     ATSCLGVDKLLVEGSTYDALLGEDDIFPFILLNERLQLSLLPSSPSLAGAEVELVDEL
                     ARESRLRKALLKVASRYDYVLIDCPPSLGLLTVNGLMAAIDGVVVPVQCEYLALEGLG
                     QLTETIERVRAALFPDLKVRGVILTMFDNRTNLSADVVREVNKHFPNQVFKSIIPRNI
                     RLAEAPSYGLPISEYAPNSPGADAYETLAKELLKGDKN"
     gene            5266..6138
                     /locus_tag="PKI33_00845"
     CDS             5266..6138
                     /locus_tag="PKI33_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNM35572.1"
                     /translation="MAQRKGLGKGLDALIPGGKSSTPAVTSSKTSGEGGVQQVPVEAI
                     KRNPRQPRVHFKEEDLNDLAASIREHGVIQPLIVSPKGDGTFILIAGERRWQASQRAG
                     LTKVPVVTRQANNQELLEIALIENVQRADLNAMEEAEAYHQLAEDFGLSHEMIAKRVG
                     KSRVAVTNTMRLTNLADAVKQALVDNTITEGHARALLGLSTQKAQTSALQTIINLSLS
                     VRQSEELIRKLTGVKPVKVKKATVSADVLDVEKRLQRSLGTKVALKHGKKGGTVTIYY
                     YSDEELDALIKKMS"
     gene            6211..>6827
                     /locus_tag="PKI33_00850"
     CDS             6211..>6827
                     /locus_tag="PKI33_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNM35573.1"
                     /translation="MKLLYNANIHTLDSANPHASAILIAGGRIIAVGSKDQLESLAHG
                     KVEKQDMKGKTILPGLTDAHIHMQFYSLGLSKVDCETKTKEECLRRVGERARTLKPGE
                     WVLGHGWQQNDWDGNFPKAAELDAVSPNNPVYLTAKSLHAAWANTSALKLANITDETP
                     DPKDGAVQRDANGKATGIMIETAMALVNAAVPEPTLAELEAAFEKA"
BASE COUNT         1682 a         1691 c         1821 g         1633 t
ORIGIN      
        1 caagcgggtg cggcgaacag gcaagtccta tgcccatccg cttgttgtgt tagtaactca
       61 agccagtgaa accaaagaac atttaaaagt tggcattgca gcagggaaaa cgaccggcac
      121 cgctgtcagt cggaatcgcg caaaacgact cttgcgtgaa gcgatgcggg acttgatccc
      181 ttccatcacc ctgcgactcg ctcaagagaa cgcctctggc tgggacttga tcctgattgc
      241 cagacccggg cttgttactg caaccctggc agatacccgc tccgcgttga ccaacctcct
      301 caaacgcgcc aagcttctcc ctgtcgatga atcctgatac cgaagaacaa cacgaacatt
      361 ttgtattgca tgattattcc catgaaccgc ggctgcgcga tatgccgcgc acgcttggga
      421 atattccgcg tattgtggtc ttgtctataa ttcggttata tcaaatggct atctcaccgg
      481 gcttgcctgc caacacctgc cgcttttacc catcgtgctc ccattatggg tatcaagccg
      541 tatataagca tggtgcattc aaaggctctt tcatggcagc ttggcgggtt ttgcgttgca
      601 atccctttaa ccccggcggg tacgaccccg tcccgtaata gtgcatcttt cacggcaaag
      661 gtcctgcctt tgccgttttt aggagtaaat ccaatttaaa tatgaaaaag ataaatctta
      721 tactcttgct cgtgctgggt gctgttttgt tgagcgcctg caccggcgga cgcgcctcca
      781 ttgttaacac atgggcgggt cttgccgccg acggcgaacg tgcttatctt tcttctggct
      841 cccacgtcta tgctgtggac gtgcagaccg gcaaagaagt ttggcgttac cccgcagaaa
      901 ccgatagcaa tatcatcttt tatgcaaatc cagtgcttac caacgatggt caattgttgg
      961 tgggaagtga aggaaacaat cacgaactgg tgagcatcaa ccccgaaact ggcaaagata
     1021 actgggcaaa acccttctct gttgctaaag ggaagtgggt ggcatctccg ctcgttttca
     1081 acgatatcgt ctacgctccc aattccgacg gctttctcta catcctcgac ttgaacggtg
     1141 agacttctgc cgaccccatc gaattgggcg gctcactttg gtccacgccc gtgacggatg
     1201 gtacttacat ttacgtcact tcgttagatc atcaccttca cattgtggat cctgcaagca
     1261 attccatcgt cagcaccatt gaccttggtg gcgcttctcc cagcagcccc gttgtgggtg
     1321 agggtggagt atatgtaggc tcatttgctt cgaacattga gtttgtcact tccaatggcg
     1381 accatgaagt tttggcaact gctacgaatt ggatctgggg aactcccgca ctcgataacg
     1441 gcactttata ctatgccgac ctcgacggtg ttctttactc cctcgatgtg aattctggca
     1501 aacagaactg gaccaatgtc aagccagatg gtcctgtcat agcaaacccg gtagttgttg
     1561 gcgatgccat ttatttcgta actgaggcgg gtactttcct cgctttggat cgtgacgcaa
     1621 agaccatttg ggaaaaagaa gtgggcggca aaatctatac cactcctgct gtttccggcg
     1681 acatggtcct cgttgcgccg tataaggcag aatttgcatt agcggcttat gacgctgaag
     1741 gcaaacaagc ctggacgttt acccccgaga aataaggaac ctagcccatg tgggaatcat
     1801 ttatcatcca accgatggtt aacctgcttt tgtggatcta tgacatttta ggtcatggac
     1861 cgcaaatgtt tggtcttgcc atcattttat tcaccatcgt gatcaggctc attacttggc
     1921 ccctcaatgc ctcacaggta aagggcgccc aagccatgca ggaattaaac agcgacaagg
     1981 aatggcaaga cattcaaaag aagtatgcca aagaccgcga aaaactggcg caggaacaaa
     2041 tgcgtattta caaagaacgc ggcattaatc ctttcgcttc ctgtttaccg accttgatcc
     2101 aatttccgat catcatcggc ttgtatcaga gcatcattcg cgctatggcg gccaccccgc
     2161 tcgacatgct caatttggcg cgcaccattt accctttcca gcatgtttcg aacatcatcc
     2221 cgcttaacag tacattcttg tggatgaacc tcggtcgccc cgaagggatt cctcttccgt
     2281 tcgatgtgtc cttcctcccg aacggtttcc cgctcttggc gttggttgtg gcaattacca
     2341 cctatgttca aaccaaactg accatgccta cgacaccgcc caaccccaat gaccaggctt
     2401 ctgcacagac tgctgccatg agcagcagca tgtccattta tatgccgctc atgttgggtt
     2461 ggtttgcgct tacatttgcc tcaggcattt cgatctactt tattaccagc aatacgttgg
     2521 gcatcattca gtatgcggta acgggtcgag ccaactggag caatttgttg cccggtggaa
     2581 acaagtctac acctgcaaag aaatagggag gcaccatgag tgagcggact acgttagaaa
     2641 ttatcgctcc aactgttgaa gaagccatcc agcaagggct cgaccagctc gggctcacag
     2701 cagatgcggt ttctgtggaa gttttagact ccggctccaa aggattgttt ggactcggtg
     2761 gacgtcaggt gcgtgttcgc atttctgtga atccgcctcc catgccggaa gttcctgcgg
     2821 ctcgacctga gccgaagcct gctccagcga aaaaagctga gtccaagccg aaagcgaaag
     2881 agccaaaaga gcccaagccg cgagctgaaa agaaacaggc tgagcccaaa acagaagcca
     2941 agcctgaacc gaaacctgaa ccgaagccgc aaaagcctgc tccacaacgc tcaacgatta
     3001 atgttgagaa tgacgcggtg ttgaatacgg cggaagaagt ggtatccaag ctgatctatc
     3061 acatgggcat gactgcacag gtctctgctc attacgatga atcagactct gccgataacc
     3121 gcaccattca agtggacgtc cgtggtgaag accttagcgc cctgatcgga cgccgcgccg
     3181 aaaccttggc ttccttccaa cacgtcgcat cgttgatcgt tggcaagcag acccagcaat
     3241 gggtacaggt catcatcgac gtggaaggct accgcgctcg ccgtgaaaag cagattcgcc
     3301 agcttgccaa ccgcatggct gaccaggtga ccaagaccgg acgcaaggtg accatggagc
     3361 cgatgccttc tagtgagcgc cgtgtggttc acatcgaatt gcgcggacat cctgccgtga
     3421 agaccgagag caccggcgaa gaaccctatc gcaaagtggt cattctgccg aaagaatagt
     3481 tcgacattta ctgatagata aaagaccgta ggtgaaaatc tacggtcttt ttgtttcttc
     3541 ttgcaagaaa gaagaaagag gaaagatttc actacggtat aattcaccta tgtctgacct
     3601 tcgcccagtg gactatcttc ttattggtca tgtggctgtc gatttaactc catcgggcaa
     3661 acaactcggt gggacggttt catacgcggc gttgacggca aaggcgatgg gcttgcgggt
     3721 tggtattgtc acctcagcgg gggaggatgc tcccttaagc cttttggatg gggttcagat
     3781 attcaacctc ccaaccgaac aaagcacgat ttttgagaac gttaaaaccg agcacggacg
     3841 caaacaaacc ttgcatcatc aagcgacgaa gatttcgttt gagcatgtcc cacagatctg
     3901 gcgagatgcg gcgattgtcc acctcgcgcc gattgcacag gaagtggacc cggttttggt
     3961 ggggcaattt ccagcttcgt gggtgggagt cacgccgcag ggctggatgc gtggctggga
     4021 cgaaaaaggt tcggtgttcg ctacggcttg ggaaaacagc gaacaggttc tcggtcaggt
     4081 gggaggcgtg gtgatgtcgc ttgaggatat caaccatgat ttggaactcg gtgaatggct
     4141 ggcgcatcac gcccgtctgc tttgtttgac ggaaggggaa gagggcgcgg tgttgcattg
     4201 gcatggcgac cgcagacggt atcgtcccat caaagtggaa gaggtggatg cgacgggggc
     4261 aggcgatatt tttgcggctg gatttttttc gcggttacat caaacaaaag acccgtggga
     4321 agcagcacgt tttgcgactc atttggcggc atattctgta acacgtgtgg gtttgcaggg
     4381 cattcccact catgatgaaa ttgaacattg caagatggag gttttgtctt gacgaagatc
     4441 tatactctgg tgaatcaaaa aggcggtgtg gggaagacca catccacgat caacttagcg
     4501 gcgtatttgg cgaagctggg gcaacgtgtg ttggtggtag acctcgaccc gcaggcaaac
     4561 gcaacctctt gtttgggcgt ggataaactt ctcgtagaag gcagtactta tgatgcgctg
     4621 ctcggcgagg atgatatttt tccgtttatc ttattgaatg agcggttgca gttgtcgctg
     4681 ttgccttctt cgccttcttt ggctggcgcc gaagtagaac tggtggatga gcttgcacgc
     4741 gagtctcgtt tgcgcaaggc attgttgaag gtggcaagcc gttatgatta tgtgttgatt
     4801 gattgcccgc cttctttggg actgctgacc gtgaatggct tgatggcagc aattgatggc
     4861 gtggtggtac cggtgcaatg cgaatatctt gcgcttgaag gtttaggtca gttgaccgag
     4921 acgattgaac gcgtgcgcgc ggctcttttc ccggacctca aagtgcgcgg cgtgattctg
     4981 acaatgttcg acaaccgcac caatctttct gccgatgtcg tacgcgaagt gaataaacat
     5041 ttccccaatc aggtgtttaa gagcattatt ccgcgtaaca ttcgtttggc agaagcacct
     5101 tcctatggat tgcctatctc agaatacgca cccaattctc ccggtgcaga cgcctacgaa
     5161 acattggcga aagaattgtt gaaaggcgat aaaaattaac cacgaagtgt cacaaagcga
     5221 cacgaaggaa aacttcaata ctttgtggtc gaaaaggtag agaacatggc tcaacgaaaa
     5281 ggtttaggca aaggtttgga tgcgctcatt ccgggtggaa agtcttcgac tccggcggtg
     5341 acgagcagca aaacaagtgg agaaggcggt gtacaacagg tgccggtgga ggcaattaag
     5401 cgcaaccctc ggcaaccgcg tgtgcatttt aaagaggaag acctgaacga cttggcggca
     5461 tccattcgtg agcatggagt catccaaccg ttgatcgtct ctccgaaggg agatggcact
     5521 ttcatcctta ttgcgggaga acgtcgttgg caggcttcgc agcgggctgg gttgacgaaa
     5581 gtccctgtgg taacgcgcca ggcgaataac caggaattac ttgaaattgc gttgatcgaa
     5641 aacgtccaac gcgcagactt gaacgccatg gaagaagccg aagcctatca tcaactggca
     5701 gaggatttcg ggctttcgca tgaaatgatt gcaaaacgcg tgggcaaaag ccgtgtagcg
     5761 gtaaccaaca ccatgcgcct gaccaatctc gcagacgcgg tcaagcaagc cttggtggac
     5821 aataccatta ccgaaggtca cgctcgtgct ttattggggc tgtccactca gaaggcgcaa
     5881 acctctgcac tgcaaaccat tatcaatttg tcgttgagcg tccgtcagtc tgaagaattg
     5941 atccgtaagt tgacgggcgt aaaacctgtg aaagtgaaga aggcaacggt cagcgccgat
     6001 gtgttggatg tagagaaacg cctccagcgt agtttgggca cgaaggttgc gctcaaacat
     6061 ggcaaaaagg gcggcacggt gacgatctat tattattccg atgaagagtt ggatgcattg
     6121 attaagaaga tgtcgtaatt cgtagaggcg cggtttccgc gccttattcc tttgtaaagc
     6181 tttgggcagg agaccctgca cctacaaaat atgaaacttc tctataacgc aaacattcac
     6241 actcttgatt ctgctaatcc gcatgcttct gcgattctca tcgcaggtgg gcggattatt
     6301 gctgtaggta gcaaagacca gcttgaaagc cttgcacatg gcaaggtgga aaagcaagac
     6361 atgaaaggta agactatctt gccgggtctg accgacgcgc acattcatat gcagttttat
     6421 tcgcttgggc tttccaaagt ggattgcgag accaagacga aggaagaatg tctacgccgg
     6481 gtgggggaac gtgcgcggac tctcaagccg ggcgagtggg tgctgggtca cggctggcaa
     6541 cagaatgatt gggatggcaa cttcccaaaa gcggcggagc tagatgcggt ctcgccgaat
     6601 aatcctgtct atctcaccgc aaagtcattg catgcggcat gggcgaatac gtccgcattg
     6661 aagttggcga atatcacgga tgaaacgccc gatccgaaag atggagccgt tcagcgcgat
     6721 gcgaacggga aagctacggg catcatgatt gaaacggcga tggcgttggt caatgctgcg
     6781 gtgccagaac caactctggc tgagttggaa gcggcgtttg aaaaagc
//
LOCUS       DAOREF010000022         6771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5785_length_6771_cov_5.241513, whole genome shotgun sequence.
ACCESSION   DAOREF010000022 DAOREF010000000
VERSION     DAOREF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_6771_cov_5.241513"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PKI33_00855"
     CDS             <1..1405
                     /locus_tag="PKI33_00855"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNM35574.1"
                     /translation="NPLSGLQVEEPSLLKTPAMALSISNFPVAARPQAGLSFAPWVFE
                     FYITEGATRFLSIFHGAFPEPEAPIVGDCEVRREPIQGNVIEIGNRIWLDENQNGIQE
                     DYEKGVGGVCINLLDATGKPVQKTTSDSNGFYGFVTEPGNYIVEVTIPEWLKFTKPNV
                     GDEEHDSDADPASGKVETDLISAPLDLDVGLVPSEKANPPVKQSAQLPPAEVGPIRSG
                     RLLYVDVHSMFLNSCFVFASASEEILPLLPQCYLVPHDEASGGAMLSIEKMKSLAEEN
                     KGSRPVDFNYASNVFAETPPEGGETALRLNSYVALLNQSAWVYDPASSSYWRYVDDTT
                     QANAGVLHPEIDRLTGRQLQFNNIIVLEVDQEVYEYQDRPIRTLLDYHLELGQGGYAY
                     LFRDGRKYDIRWTTKARAYEKQTGQARPLYFVDANGDPVSLKPGNTWFIVATPYSYFT
                     PEDGIWNFRFVPPEGVK"
     gene            1438..2091
                     /locus_tag="PKI33_00860"
     CDS             1438..2091
                     /locus_tag="PKI33_00860"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNM35575.1"
                     /translation="MKILFEANANPVNAAPMKKYMRDQFEYLGIKSPQVKSLFSGYIK
                     QNSLPPVEDLSTIVRELWNLPQREFQYLAINLLDKMEKKLPEDFIVTLEYLITHKPWW
                     DTVDLIAGHSVGMMFKRYPKVKAQYLKKWRKSENIWLRRTTLLFQLGYKKETDFGLMC
                     ELIQENLGSDEFFINKAIGWALRQYAWTNPSAVKKFVKATKELSPLSRKEALKNIGA"
     gene            complement(2088..2501)
                     /locus_tag="PKI33_00865"
     CDS             complement(2088..2501)
                     /locus_tag="PKI33_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNM35576.1"
                     /translation="MTEQSLYELLGGEEGVRTLVDRFYDLMDSSPEAKDVRALHAKSL
                     KQAREKLFMFLSGWSGGPPLYIEKFGHPRLRMRHMPFVIGTRERDQWLWCMNRAVDAG
                     NYHSAVVEHLKQRFSEIADFMRNQDETEQGPLLQL"
     gene            complement(2566..5445)
                     /locus_tag="PKI33_00870"
     CDS             complement(2566..5445)
                     /locus_tag="PKI33_00870"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNM35577.1"
                     /translation="MSDVFISYSRRDKVFTQKLYEALRDSGREIWADWDSIPAASDWF
                     AEIKDGVEKANTIVFVLSPEWLKSNECRKEYDHAVAMGKRLLPILYQVVDPKEVPSEL
                     AKINWVYMRDTDDFGQAFQTLSAAMDTDLEWVKTHTRIQIRAIEWDKKSRDHSFLLRG
                     NDLTDGEQFIAQGAQKSPEPTVLHGEYVLASRKDATRRQRITLAGVTVALVVSVALGI
                     VAFFQRQAAVAARAEAVVERDRAVAAEKIAEDERDRADVNAEVAYSRELAAASLNSLN
                     HDPELSLLLGLQSVAITDKTGMPIQPMSEDALRRSVQTSRIRKTWLAHSGSVLDIAIS
                     PDGTKIASASDDLTVKIWDLSGKLLLTIPDFSEEVDSVVFSPDGSQILTSSGQEAVAW
                     DAVTGEKLVEYKGHVSYVVQAVYSPDGKFIATASSDATAIMWDALSGESLVVLGGHET
                     ELSDIAYSPDGEMIASTDYNGAMYVWSTSSKSLLYMLSEGEGSVILDVEFSPDGKYIL
                     ISDNYFQTIVLVAQTGKVISAQNVHTDFVTNAVVSPDGAFMASASTDNTVVVSSITAS
                     AVLFGQSISADPDYIDISKVMFRFTDSTDINTMIYTPDGKYLITGNDEGQIKIWDANP
                     ISDYEVSGFRPHTARIDGMEFSPDGEHYVTVARDGKAFIWSLTDQSSIELVGHQNVIQ
                     DVAYSSDGMQIITGGFDGHAIIWDAKTGDKLITLSKDDQLVTGVNFSPDGKLAVGSLG
                     DNTVVVWDAVSGDEVRVLQSKDMYAYINELAFSPDGKTLAVSSDAGFVVLWDVATWNQ
                     IDVLAGHTDWVKGLDYSSDGTHMVTASYDTTAIIWDVATRTPVLKLEGHSQGVNDVTY
                     SPDDTLVATASVDGTAKLWNANTGELLVTYQTSADQILSVAFSADGKYLATGSETGVV
                     RIYYVYAEDALELAKSRITRTFTEEECLKDLHEEACPANLPQPQQ"
     gene            5613..>6771
                     /locus_tag="PKI33_00875"
     CDS             5613..>6771
                     /locus_tag="PKI33_00875"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNM35578.1"
                     /translation="MSKENEAVILSAARTPIGKFQGALSGIPAPKLGAIAVEEAVKRA
                     GIDPSEVEEVFMGNVVQAGNGQAPARQAAIYGGIPATVSATAINKVCGSGLKAAMMSA
                     QAIRAGDAELFIAGGFESMSRAPYLVSGRMGELKFGNSQMTDALLNDGLWDPFENWGM
                     GNAAEFIADEYEVTRAAMDEFALRSHLKAVEAQEAGRFNAEIVPVQIPGRKGEVTTVA
                     HDEGPRKDTSLESLAKLKPVFKADGKVTAGNSSSMNDGAAATIISSRGYAEKKGLKPL
                     ARIVGYAQAAMEPKYLFAAPAFAMPKLLKKIGWTLKDVDLIELNEAFAAQVLADGYAL
                     ADQGWDWDKVNVNGGAIALGHPLGASGARGLTTLIYALQNRGLKRGIASLCL"
BASE COUNT         1853 a         1689 c         1528 g         1701 t
ORIGIN      
        1 aaatccgctt tcggggttgc aagtggaaga accaagctta ttaaaaactc cagccatggc
       61 actctctatt tcaaattttc ccgtagcggc tcgcccacag gcagggttat cgtttgcgcc
      121 atgggtcttt gagttttaca tcaccgaagg cgcaacccgc ttcctctcca tttttcacgg
      181 agcatttccc gagccagaag ctcccatcgt tggcgattgc gaagtgaggc gtgagcccat
      241 tcaaggcaat gttattgaga tcggaaatcg tatctggttg gatgaaaacc aaaacggcat
      301 tcaagaagat tacgaaaaag gtgtaggggg agtctgtatc aatttattgg atgcgaccgg
      361 gaaaccagtt cagaaaacaa ccagcgactc aaacggtttt tacggcttcg tcacagagcc
      421 gggaaactat atcgttgaag tgaccattcc agagtggttg aaatttacga agccaaatgt
      481 tggagatgag gaacatgaca gtgacgcgga ccctgcttcg gggaaggtcg agacggatct
      541 tatctccgcg cctcttgact tagacgtggg cttggttccg tccgaaaaag cgaatccacc
      601 cgtgaagcaa tcagcccaac tcccccccgc tgaggtggga cccattcgct cgggacgctt
      661 gttatacgtg gatgttcaca gcatgttctt aaattcatgt tttgtctttg catcggcatc
      721 ggaagaaatt ctgccattgt tgccacaatg ctatttggtg ccacatgacg aagccagtgg
      781 cggtgcaatg ctaagcattg aaaagatgaa atcgctggca gaagagaata aaggctcacg
      841 accggttgat ttcaattatg ccagcaatgt tttcgcagag acgccacccg aaggtggtga
      901 gacagcgctg cgtttaaatt cttatgtagc cttgctcaat caatctgcat gggtgtatga
      961 ccccgcctcc agttcgtatt ggcgttatgt agatgacacg acacaagcaa atgccggagt
     1021 cttacatcca gaaatagacc gcctgacagg caggcagcta caattcaata acatcatcgt
     1081 attggaagtt gaccaggaag tctatgagta tcaggataga cccatccgca ccttgctaga
     1141 ctatcacctc gaacttgggc aaggtgggta tgcttatctc tttcgagatg gcagaaaata
     1201 tgatattcgc tggacaacca aagcccgtgc ttatgaaaaa caaaccgggc aagcccgccc
     1261 gttatatttt gtagatgcga atggagaccc agtctcctta aagccaggca atacctggtt
     1321 catcgttgcc acgccctatt cgtactttac gcccgaagat ggcatttgga atttccgttt
     1381 tgtgccgcct gaaggtgtga aataaaagag gaaattatgc atccatatgt acgttctttg
     1441 aaaatacttt ttgaagccaa tgccaacccc gtcaacgccg cgccgatgaa gaaatatatg
     1501 cgcgaccaat ttgagtatct gggaataaaa agcccgcaag tgaagagtct gttttctgga
     1561 tacatcaagc aaaacagttt gccgcctgta gaagacttaa gcaccatcgt ccgcgaatta
     1621 tggaatctcc ctcaacgcga gtttcagtac cttgccatta acttgctcga taaaatggaa
     1681 aagaaactac ctgaagattt cattgttacg cttgaatacc ttatcactca caaaccctgg
     1741 tgggacacgg ttgacctcat tgcagggcac agcgttggca tgatgtttaa gcgctaccca
     1801 aaggtcaagg cacagtattt gaaaaagtgg cgcaaatctg agaacatctg gctcagacgc
     1861 accactctgt tgtttcaact cggctataaa aaagaaacag attttggact aatgtgcgag
     1921 ctaattcaag agaaccttgg ctctgatgag ttcttcatca acaaagccat cggttgggca
     1981 ttacgccaat atgcgtggac caatcccagc gctgtgaaaa aattcgtgaa agccacaaag
     2041 gaattaagtc cacttagccg aaaagaagcg ctgaaaaata ttggcgctta aagctgcaaa
     2101 aggggacctt gttcagtttc atcctgattg cgcatgaagt ctgcaatttc ggagaagcgc
     2161 tgtttgagat gctcaaccac agctgagtga taattccctg cgtccacggc gcgattcata
     2221 caccataacc actggtcacg ttcccgcgta ccaatgacaa acggcatgtg ccgcatccgt
     2281 aaacgaggat gcccaaattt ctcgatatat agtggaggtc cgcctgacca gccagagaga
     2341 aacataaata acttctcgcg agcttgcttc aagcttttgg catgcaaagc acgtacatct
     2401 tttgcttccg gcgaagaatc catgaggtca tagaagcggt caaccagggt acgcacaccc
     2461 tcttcaccgc cgagtaattc ataaagggat tgttcagtca tagcggcatt gtaatcgaaa
     2521 gaaataaaaa ccgccttcta tctactagaa ggcggtttac tttagttact gttgtggctg
     2581 agggagattc gccgggcaag cttcttcgtg caaatctttc aaacattctt cttctgtaaa
     2641 tgtacgcgtg atcctgcttt tggcgagttc aagggcatct tcagcatata catagtagat
     2701 acgcaccacg cccgtctcgc tgccagttgc caaatatttt ccatcagcgc taaatgccac
     2761 acttaagatt tggtccgccg aggtttgata tgtgaccaat aactccccag tatttgcgtt
     2821 ccaaagcttg gcggttccat ctacacttgc agtagccacc agcgtatcat ctgggctgta
     2881 tgtcacatca ttcactccct gggaatgtcc ctccaatttc aaaacagggg tccgcgttgc
     2941 aacatcccaa ataatcgccg tggtgtcata acttgccgta accatatgcg taccatcaga
     3001 actgtaatcc aagcccttga cccaatcggt atgcccagcc aatacatcaa tttgattcca
     3061 tgtagcaaca tcccatagaa caacaaatcc ggcatcgcta cttacagcaa gagttttacc
     3121 gtctgggctg aaagctaatt cgttgatgta tgcatacata tctttcgact gcaaaacacg
     3181 tacttcgtca ccagatacag catcccaaac cactactgta ttatcaccca atgagcctac
     3241 agccaattta ccatcagggc taaaattaac cccagtaacc aactgatcat ccttagaaag
     3301 tgttattaat ttatcgcctg ttttagcatc ccaaataatg gcatgaccat caaaaccgcc
     3361 agtgattatc tgcattccgt cagagctata ggctacatct tgaatgacgt tctggtgtcc
     3421 gaccaattca atggacgatt gatccgtcaa tgaccagata aacgccttcc catctcgagc
     3481 tacagtgaca taatgttcac catctgggct aaactccatg ccatcaatac gtgctgtatg
     3541 tggacggaat ccgctcacct cataatcact tattgggttt gcatcccata tttttatttg
     3601 cccttcatca tttcctgtaa tgaggtactt tccatcaggg gtatagatca tagtattaat
     3661 atctgtagaa tctgtaaacc tgaacatgac cttactaata tctatataat ctgggtcggc
     3721 agaaatactt tgtccaaaca atactgcgct tgcagttatt gagcttacaa ccacggtatt
     3781 gtctgtagag gcacttgcca taaatgctcc atccgggcta accacagcat tggtcacaaa
     3841 gtcggtatga acattctgcg ctgagattac cttgccagtt tgggcaacaa gaacaatcgt
     3901 ttgaaaataa ttatcagaaa taagaatgta cttaccatct gggctaaatt ctacatcaag
     3961 gataacagaa ccttcccctt ctgaaagcat atacaataaa ctcttcgaac tggtgctcca
     4021 aacatacata gctccgttat agtctgtcga ggcaatcatt tcgccatcag ggctatatgc
     4081 gatatcagac aattctgttt catgcccacc gagcactacc aagctttctc cagataaagc
     4141 atcccacata attgcagttg catctgaact agcggtggca ataaatttcc cgtctgggct
     4201 atagaccgct tgtacaacat aggatacatg acctttatat tcgaccaatt tttcgcctgt
     4261 gacagcatcc catgcaactg cttcctgtcc gcttgaggtc agaatctgtg aaccatctgg
     4321 actaaacaca acgctatcga cttcttctga aaaatcaggg atggtcaata ataatttgcc
     4381 tgacaagtcc cagatcttaa ctgttagatc atcactggca gatgcaatct tagttccatc
     4441 agggctaatt gcaatatcga gaactgaacc tgaatgtgcc aaccaggttt ttcgaatccg
     4501 tgatgtttgc acagaacgtc tcagggcatc ttcactcata ggttgaattg gcatccccgt
     4561 tttatctgta atagcaacag attgcaaacc taacaacaga ctgagttcag ggtcatggtt
     4621 caatgaattc aaagaagcag cggctagctc acgagaatat gcaacttctg cattaacgtc
     4681 cgcacgatct cgttcatctt ccgcaatttt ttcggcagcc acagcacgat ctctttccac
     4741 cacagcttct gcacgcgcag caacagccgc ctgccgttgg aaaaatgcca caatgcccaa
     4801 agcaaccgaa acaaccagtg caaccgttac acccgccaaa gttatccgct gacggcgagt
     4861 ggcgtccttt cggctggcaa gcacatattc accgtggaga acagtaggtt caggtgattt
     4921 ctgtgcccct tgagcaataa attgctcccc atccgttaga tcatttccac gcaataagaa
     4981 tgaatggtca cgacttttct tgtcccattc aatcgcacgg atttgaatac gagtatgggt
     5041 ttttacccat tccaagtctg tatccatggc ggcagaaagg gtttggaatg cctgaccaaa
     5101 atcatctgta tcacgcatgt agacccagtt aatcttcgcc agctcagacg gaacttcctt
     5161 tgggtctact acttgatata ggataggcaa taatcgtttc cccattgcca cggcatgatc
     5221 atattctttg cggcattcgt tcgatttcaa ccattctggg cttaacacaa aaacaatcgt
     5281 gtttgctttt tccaccccat ctttgatctc agcaaaccaa tccgatgccg ctgggatcga
     5341 gtcccagtcc gcccaaatct cacgaccaga gtctcttagt gcttcatata gtttttgagt
     5401 gaatactttg tcgcgccttg aataagaaat aaatacatct gacatgtcgc tctcctgatc
     5461 ctatatatcc ataagaaatg tgtcgattat atagtaggct ttgttatgga aagacctaca
     5521 agtttgaaag cagagaatga aagaacttaa cagaatttct gccctatggc ggctctcgcg
     5581 tataatacga atatcacccc acaggaggta cgatgtccaa agaaaatgaa gcggttatat
     5641 tgagcgcagc ccgcacaccg atcggaaaat tccaaggcgc gttgagcggt attccagcgc
     5701 cgaaactcgg tgcgattgcc gtggaagaag cggttaaacg cgcaggcatc gaccccagcg
     5761 aagtggaaga agttttcatg ggcaatgtgg tgcaggctgg caatggtcag gcaccggcac
     5821 gacaagcggc aatttacggc ggcatccctg caaccgtcag tgcgactgcc attaacaagg
     5881 tctgcggctc gggcttgaaa gcggcgatga tgtctgccca agccattcgg gcgggtgatg
     5941 cggagctctt catcgccggt gggtttgagt cgatgagtcg cgctccgtat ctcgtcagcg
     6001 ggcgcatggg cgagttgaaa ttcggcaact cccaaatgac cgatgccctg ctcaacgacg
     6061 gtctgtggga tccgtttgaa aattggggca tgggcaatgc cgcagaattt atcgcggatg
     6121 aatatgaagt gactcgcgcc gcgatggatg aattcgcgct tcgctctcac ctcaaagctg
     6181 tagaggctca agaggcggga cgtttcaacg ctgaaatcgt tcccgtccaa attcccgggc
     6241 gcaaaggtga agtgaccaca gtggctcacg atgaaggtcc gcgcaaggat acatcgctag
     6301 agtcacttgc caagctcaaa cccgtcttca aagcggacgg aaaagtgacg gctggaaatt
     6361 catcgtcgat gaacgatggc gcggcggcga cgatcatctc ttcgcgtggc tatgctgaaa
     6421 agaaagggct caagccgctt gcaaggatcg ttggctatgc ccaggcagca atggaaccga
     6481 aatatctttt tgcagcacct gcatttgcca tgccaaagtt gttaaagaaa atcggctgga
     6541 cgttgaaaga tgttgacctg atcgagttga acgaagcctt cgccgcccaa gtcctcgcgg
     6601 acggttacgc tctcgccgac cagggctggg actgggacaa ggtcaacgtg aatggcgggg
     6661 caattgcgct cggtcaccca ctgggagcca gcggcgcacg cgggttgacc accctcatct
     6721 acgccctgca aaaccgcgga ctaaaacgag gtattgcatc cttatgtctt g
//
LOCUS       DAOREF010000023         6770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5786_length_6770_cov_5.557260, whole genome shotgun sequence.
ACCESSION   DAOREF010000023 DAOREF010000000
VERSION     DAOREF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5786_length_6770_cov_5.557260"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="PKI33_00880"
     CDS             complement(<1..1604)
                     /locus_tag="PKI33_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNM35579.1"
                     /translation="MSNSNIAEKPIYLDTSRPREERVKDLISHLTLEEKVGLMSHPTN
                     GIPRLNIPAYNYWNEALHGVARNGRATVFPQAIAMAATWDKDLIHKIGNAISDEGRAK
                     YHAALNRNGYTQQYQGITFWSPNVNIFRDPRWGRGQETWGEDPFFTGEMAAEFVKGLQ
                     GNDPTYLKTAACAKHFAVHSGPEKDRHSFNAIVTKRELYDTYLPAFKKLVTEAKVEAV
                     MGAYNRTLDEVCCASKLLIEDILRGEWGFDGHFVSDCMALSDFHLNHKVTKDAAESAA
                     LALKYGCDLGCDHVFNELPAAIERGDTTEALVDRALERTLRTRFKLGMFDPAEDVPFA
                     SISTDVVASDTHRQLAYKAATEAVVLLKNQNNVLPIKPSTKKVFVTGPTATSVEVLLG
                     NYYGFNNKMVTLLEGIVGRIPEGMGLEYTAGTPLKHAREIKESWAPQMAQSADFAIVC
                     AGFNSFLEGEEGESLLSAQNGDRESISLPQSQIDYIKELAIHGARIILVLTGGSPIAL
                     GEVEDLVEAILFVWYPGMEGGRAVADV"
     gene            complement(1620..2786)
                     /gene="xylA"
                     /locus_tag="PKI33_00885"
     CDS             complement(1620..2786)
                     /gene="xylA"
                     /locus_tag="PKI33_00885"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNM35580.1"
                     /translation="MSKYSPSPENKFTFGLWTVGNVGRDPFGSPVRDGKSPADLVRLL
                     GEVGAYGVNFHDNDLIPIDATPSERDAILKEFKKALADTGIIVPMATTNLFSEPIFKD
                     GAFTSNDSKVRAYALQKTMNAIDLGVELGAKTYVFWGGREGTESDATKDPITSIKRSR
                     EAMNFLCEYVLDKKYDLKFALEAKPNEPRGDIYNSTTGHMLAFIETLDHPDMVGVNPE
                     VAHEHMAGLNFVHGVAQALEAGKLFHIDLNDQAFGRYDQDFRFGAVNIKSAFFLVKLL
                     EDNKYNNPRHFDAHAYRTEDYEGVKDFARGCMHTYLVLKEKAAQWNADKEIQALVAEI
                     NADDGSMAQYFGKYSSAKASALKAQAFDRNAIASRGLKYERLDQLTIDVLLGVR"
     gene            complement(2953..3828)
                     /locus_tag="PKI33_00890"
     CDS             complement(2953..3828)
                     /locus_tag="PKI33_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNM35581.1"
                     /translation="MENVRESDKAMLNNYKFTTTVLRILVYFMLFALLVVTIVPIWLL
                     IVNATRSTTEIQQGLSIMPSTHLIENYNILLGKGLNLPRGFANSTFLAVSSTVVSVYF
                     SMLTAYGIVVYDFKGKHAFSNFIVVLVMIPMQLSIIGFYQYMSKLGLTDNYASLILPL
                     IANAGGVFFGKQYLESMIIQDLIDAARIDGASEMAIFHQVMMPLAIPGAVTMGIFAFI
                     TSWNNFFNAFILISSIEKYTLPMLVQTLRGDVYRTEFGAIYLGLAATIVPIILLYIFF
                     SRYVVSGIAIGAVKE"
     gene            complement(3812..4750)
                     /locus_tag="PKI33_00895"
     CDS             complement(3812..4750)
                     /locus_tag="PKI33_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM35582.1"
                     /translation="MGLIKLKKHQYGYIFIAPFIIGFLAFGLYPVYNTLALSFTDATL
                     MTKSAHFVGFKNFERLFADDVFLGAVKNTWLLWALNFIPQVGIAMLLSVWFTDARLKI
                     KGVGVWRAVFFLPNLLVPASVAALFFAFFSFYGPVNQFMVRFGFLPEAMHYLQSVPVT
                     RGLVIFIQWWMWFGQTVIIVMAGMTSIPVSLYEAAMVDGATTSQMFRRITLPLLKPIL
                     IYIFVTSLVGGMQMFDIPFLLTDGRGSPNSSIQTNNILMYMKFASSKGHIGAASSVGV
                     LVFLMTSICALGIFYFLRERDTVSEKPKKSLFGKRS"
     gene            complement(4842..6305)
                     /locus_tag="PKI33_00900"
     CDS             complement(4842..6305)
                     /locus_tag="PKI33_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM35583.1"
                     /translation="MKRLSYILSLLVVVSMFLAACAPAATEEAPAATEAAAEATDAPA
                     ATEAAPAELSGTLNVWSFTNEIRTMAIAFEGVHPNVDVVYTMIPMTNGEYQTKVKAAA
                     GTADSPDVVALEAAFVKEWVESDLLADQSDLLPLAEELKTFPSVVEVGTYDGITKAYS
                     YQATPGAFFYRRSIAKECLGTDDPDAVQAMVADLDKFVETAAKIKECGTGDYFTVGTS
                     GEMFNPFLANREQPWVVDGELVIDPMVLEYVKFAKLMRENGYESQATQWSEGWFAGMN
                     DTLKDANGTPKKVFSYFLPTWGLPYVLIPNSKSDTTDTGGDWAMINGPMAYQWGGTWV
                     GALEGSPNAELAKEFIKFVALNEDNLTNWATGVYTNEYLKAIDPETPDDQAQAPGDFV
                     SSQVVVEKITSTFDGSDLAKWLGGQNSYAAFAKAAPDVNGALLTGSDDAIQRALNDPL
                     ASYLNGDITEAEMYTQWLDAVRNEFPDLVIPEPPVTE"
BASE COUNT         1774 a         1596 c         1708 g         1692 t
ORIGIN      
        1 acatcggcaa cagcgcgacc accttccatg ccgggatacc aaacgaagag aatggcttcc
       61 acgaggtctt ccacttcgcc caaggcgatt gggctgccac ctgtgagcac aaggatgatg
      121 cgcgcgccat gaatagcgag ttctttgatg taatcaattt ggctttgcgg gagcgagata
      181 ctttcacggt cgccattttg ggcagagagc agtgactcgc cttcttcacc ttcgagaaag
      241 gaattgaagc ccgcacacac aatcgcaaaa tctgcagatt gtgccatttg cggcgcccaa
      301 gactctttaa tctcgcgcgc atgtttcaat ggcgtaccag cagtatattc caaccccatg
      361 ccttcaggaa tgcgccctac aataccttcc aacagagtga ccatcttgtt gttgaagccg
      421 taataatttc caagcaacac ttcaacgctt gtggctgtgg gacccgtgac aaaaactttt
      481 ttcgtagaag gtttgatagg cagaacgtta ttctgattct tgagcaacac caccgcttct
      541 gttgcggctt tgtatgccaa ttggcgatga gtatcacttg caaccacatc cgtagagatg
      601 gatgcgaaag gcacatcttc ggcggggtca aacatgccga gtttgaagcg cgttcgcaat
      661 gtgcgttcga gggcgcggtc taccaaggct tcggtcgtat ctccgcgttc gatggcggcg
      721 ggaagttcat tgaagacgtg gtcacagcca aggtcgcagc catatttcaa cgccagtgca
      781 gcggattccg ccgcatcttt cgtgaccttg tggttgagat gaaaatctga cagcgccatg
      841 cagtctgaaa caaagtgccc gtcaaagccc cattctccgc gcaagatgtc ttcgatcaga
      901 agtttgctgg cacaacacac ttcgtccaag gtgcggttgt aggcacccat cacagcttcc
      961 actttggctt cggtaacaag ttttttgaaa gcaggcaggt aggtgtcgta taactcacgt
     1021 ttggttacaa tggcattaaa ggagtggcgg tctttttctg gaccagaatg cacagcaaag
     1081 tgttttgcac aggcggcggt tttgagatag gtggggtcat tgccttgcaa gcctttgacg
     1141 aactcagccg ccatttcacc ggtaaagaag ggatcttcgc cccaggtttc ttgtccgcgc
     1201 ccccaacgtg ggtcacgaaa tatattcaca ttcggtgacc agaaagtaat gccctgatat
     1261 tgttgggtgt agccattgcg gtttagagcc gcatggtatt tagcgcgacc ttcgtcactg
     1321 atagcattgc ctattttgtg gattaagtct ttgtcccaag tagcagccat ggcaatggct
     1381 tgcggaaaga ctgtggctct tccgttacga gcaacgccat gcagcgcttc attccagtag
     1441 ttgtatgcag ggatgttcag gcgcggaatc ccattggttg ggtgactcat cagccccact
     1501 ttttcttcca aagtaaggtg gctgatcaag tcttttacac gttcttcccg tggacgcgac
     1561 gtatcaagat agatgggttt ttccgcgata ttcgagttac tcattcaagg gctcctacgt
     1621 tatcgcacgc caagtagtac gtcgatggtc aactggtcga ggcgttcgta tttcaatccg
     1681 cgagaggcga tggcattgcg gtcgaaggct tgagctttga gagcgctggc tttggctgag
     1741 gagtacttgc caaagtattg cgccatggaa ccgtcatctg cattgatctc tgcaacgaga
     1801 gcttggatct ctttatccgc attccattgc gcggcttttt ctttaagaac gagataggtg
     1861 tgcatacagc cacgcgcgaa atctttaacc ccttcgtagt cttcggtgcg atatgcatga
     1921 gcatcaaagt gacgtggatt gttgtattta ttgtcttcga gtaatttgac gaggaagaaa
     1981 gcgctcttga tgttgaccgc accaaagcgg aagtcttgat cgtagcgacc gaaggcttgg
     2041 tcgttcaagt caatgtggaa gagtttgccc gcttccaacg cctgggctac gccgtggacg
     2101 aagttgagcc ctgccatgtg ttcatgtgcc acttcagggt tgacgccgac catatcggga
     2161 tgatcgaggg tttcaatgaa tgccaacata tgcccagtgg tggagttgta aatgtcgccg
     2221 cgcggttcgt tgggtttggc ttcgagcgcg aacttgaggt catatttttt gtctaataca
     2281 tattcgcaga ggaagttcat cgcttcgcgt gagcgtttga tggaggtgat ggggtctttg
     2341 gtggcgtcac tctctgtccc ttcgcgaccg ccccagaata cataggtttt ggcaccgagt
     2401 tccacgccaa gatcgatggc gttcatggtc ttctgcaacg catatgcgcg gacttttgag
     2461 tcgtttgatg taaatgcacc atctttgaaa atgggttcag agaacaaatt ggtggttgcc
     2521 atcggcacga tgatgcctgt atcggcaagc gccttcttga attccttcag aatggcatct
     2581 cgttcagagg gcgtagcatc aatgggaatc aggtcgttat catgaaagtt gacaccgtat
     2641 gcgccaactt ctccgagcag gcgaaccaag tctgcgggag atttgccatc gcgaaccggg
     2701 ctaccgaatg ggtcgcgtcc aacattgcca acggtccaca gaccgaaggt gaacttgttt
     2761 tcaggggatg gggaatactt ggacattaga ttactccttg gtttttactg atttttagca
     2821 atactctccc agccccgcct ccacatggag gcggggaacg cataaggaga tagaacaaat
     2881 gaatgaagca aaactaagat ggagggtaaa gtgtaagagg gtagaacaag aaaagttaaa
     2941 gtatgaggcg ctttattcct ttacagctcc aatggcaatg ccgctaacca cgtatcgtga
     3001 gaagaaaata taaagaagaa tgatcggaac aatggtggca gccaagccaa ggtaaatggc
     3061 gccaaattcg gttcgataca catctccgcg cagggtttgc accaacatgg gaagtgtgta
     3121 tttttcgatc gaagagatca ggatgaaggc gttgaagaag ttattccaag atgtaatgaa
     3181 ggcaaagata cccatggtca ccgcaccggg aatggcaagc ggcatcatca cttgatggaa
     3241 tattgccatt tcactggcgc cgtcgattcg agcagcatcg atcaagtctt gaatgatcat
     3301 ggattctaaa tattgttttc caaagaacac cccgcctgca ttggctatca gcggcaggat
     3361 cagagatgcg taattatctg ttagccctag tttcgacatg tactgataaa agccgatgat
     3421 cgagagctgc atgggaatca tgaccaacac gacaatgaag tttgagaagg catgtttgcc
     3481 tttgaaatca tagaccacaa tgccataagc agtcaacatg gaaaaataga cagataccac
     3541 tgtggacgat accgctaaaa aggtgctatt tgcaaaccct ctgggaaggt tcaacccttt
     3601 tccaaggagg atgttgtaat tttcaataag atgcgtactg ggcataatgc tgagaccctg
     3661 ttggatctcg gtggttgagc gggttgcatt gacgatcaat agccagatcg gcacgatcgt
     3721 aacgaccagt aacgcgaaca gcataaaata aacaagaatt cgaaggacgg tggttgtgaa
     3781 tttgtaattg ttcaacatgg ctttatcact ttcacgaacg ttttccaaag agagacttct
     3841 tgggtttttc tgaaactgta tctctttcgc gcaagaagta gaaaattccc agtgcacaaa
     3901 tgctggtcat aagaaatacc agcacaccaa ccgatgaagc tgcaccaata tgtcccttac
     3961 tactggcgaa cttcatgtac atcaagatgt tgttggtttg aatggagcta ttgggtgagc
     4021 ctcttccatc cgtgagaagg aaaggaatat cgaacatttg cattccgccc acaagagatg
     4081 tgacaaagat atagatcaaa atgggcttga gcaaaggcag agtgatacga cggaacattt
     4141 ggctggtggt tgcgccatct accatggcag cttcatataa tgaaacgggg atggacgtca
     4201 tccctgccat gacgatgatg acagtttgcc cgaaccacat ccaccattga atgaagataa
     4261 ccaatccgcg cgtaaccggt acgctttgga ggtaatgcat ggcttcgggc aaaaaaccaa
     4321 aacgcaccat aaattgattg acaggtccgt aaaaagaaaa gaaggcgaaa aataaagccg
     4381 ctacagatgc aggtaccagc aggttgggca aaaagaaaac cgcccgccat acccccacac
     4441 ctttgatctt aagtcttgca tcggtgaacc atacggataa caacatggcg atcccgacct
     4501 gtggaataaa gttcagcgcc caaagtaacc aggtattctt tacagcacca aggaagacgt
     4561 catcagcaaa caaccgctca aaatttttga aacctacaaa atgagcactt ttggtcatta
     4621 gagttgcatc agtaaagctc aatgccaaag tgttatagac cgggtataag ccgaaggcaa
     4681 gaaaaccaat aatgaacggt gcaataaaaa tatacccgta ttgatgtttc ttaagtttga
     4741 taagtcccat gacgtctcct caacaaaata aaaattgtca gtggtgttct gacgggttgg
     4801 gtcagtgttt tgcactgacc caaccactac ttcacattct cttattcagt gacaggaggt
     4861 tcagggataa cgagatccgg gaactcattg cgaactgcat caagccactg ggtgtacatt
     4921 tcagcttcag tgatgtcgcc attgaggtag ctggcgagcg gatcattgag ggcacgctga
     4981 atggcatcgt cagaaccggt caacaaagca ccgttgacat ctggagcagc cttggcgaaa
     5041 gcggcatagg agttctgtcc gccgagccat ttggcaagat ctgaaccatc gaaggtgctg
     5101 gtgatcttct caaccacaac ctggctggaa acaaagtcgc caggagcctg agcttgatca
     5161 tctggggttt caggatcgat cgccttgagg tattcgttgg tgtaaacgcc ggttgcccag
     5221 ttggtcaggt tatcttcgtt aagagctacg aacttgatga attccttagc caattcagca
     5281 ttggggctgc cttccaaagc gccaacccaa gtaccacccc attgatacgc cataggacca
     5341 ttgatcatcg cccagtcgcc gccggtgtcg gtggtgtcag acttggagtt ggggatcaac
     5401 acgtaaggaa gaccccaagt ggggaggaag tagctgaaga ccttcttcgg ggtgccgttg
     5461 gcatccttca aagtatcgtt catgccagcg aaccaacctt cactccactg agtagcctga
     5521 gattcgtagc cgttctcgcg catgagcttg gcaaacttga catattccaa gaccatcggg
     5581 tcgatcacga gttcaccatc cacaacccag ggctgttcgc gattggcgag gaaggggttg
     5641 aacatttcac cagaggtacc cacggtgaag taatcgccgg tgccgcattc cttgatcttg
     5701 gcagcggttt caacgaactt gtcgagatcc gcaaccatgg cttgcactgc atcaggatca
     5761 tcggtgccga ggcattcctt ggcaatgctg cggcgataga agaacgcgcc gggggtcgcc
     5821 tgataagaat acgccttggt gatgccatca tacgtaccaa cttctacaac agaggggaag
     5881 gtcttgagtt cttcagccaa aggcagcaag tcgctctggt cagccaaaag atcagattca
     5941 acccattcct tgacgaaagc cgcttcaagc gcgaccacat cgggggaatc cgcggtaccg
     6001 gcagcagcct taaccttggt ctggtattca ccattggtca tcgggatcat ggtgtaaaca
     6061 acatcaacat tcgggtgaac accttcgaac gcgattgcca tggtgcgaat ttcattggtg
     6121 aacgaccaaa cattcaaagt accagataat tcagcagggg cagcttcagt ggctgcaggg
     6181 gcatcagtag cttcagcggc tgcttcagtg gcagcggggg cttcttcagt cgctgctggt
     6241 gcgcatgctg ctaagaacat gctaacgaca accagcagag ataagatgta agataatcgt
     6301 ttcatcttcg ttctccttgt taataattat ggtggtatat ttccaagttg aagtccgcat
     6361 ccttccatag agtcgggctt cgacttcttg gcgtccgatg tcattcagat atcggacgct
     6421 cgcatttcaa gtacatgaaa tttgcgagta acaatatgtg atgccagcca cctccttttt
     6481 catttcacaa aataaattcc aaactgcact actgtacgga tcctacagac cggctctaaa
     6541 tcggacctgt aagtgggtac caaaacctgg gctttcactt actcttaggg agtttccggt
     6601 aacgttaccg gaaactcgta taaaaaaatc catttccgtc caccactatc ttacgattgg
     6661 acggtttgga ttaatttttg acaggatgcg cggacgacta actgtgtctg catcttatga
     6721 acatcaaact ctaaaggttc gccttcaatt aacttgatca acatttgtac
//
LOCUS       DAOREF010000024         6723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5850_length_6723_cov_4.935963, whole genome shotgun sequence.
ACCESSION   DAOREF010000024 DAOREF010000000
VERSION     DAOREF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_6723_cov_4.935963"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1927
                     /locus_tag="PKI33_00905"
     CDS             65..1927
                     /locus_tag="PKI33_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM35584.1"
                     /translation="MKPLKISLSVKLVFAFLFLSLIPLGIVIYFNAVFGSEHLQEDAD
                     RQLGSTAKLAANNINVFLTGGLDHIQSTAALAEFEEYLSLPASERKGSEIEQEVYQNL
                     QATVLLNPAYITSVGVMDLNGVNLVDTNPTQIGERENEKEFFIQTIETKAPYASNAEL
                     DEGEYSIYFSAPIFDDNNKTLGIVRIRYDANVLQDILLKTAEDSSIEGVVIDLFDENH
                     VALAVTDAPEDILKILVLPSDEKLKELQEAGRILQGSAEEVALNDPDLETHLRNAEQT
                     PFFTIESEGEEAATAFIQSKEWIVLAGQAQSIYQAPLQEQLRIEITIGLALLVIVSAL
                     ALLMARSISRPIIDLSGVAKDIANGNIDLQAKVQTTDEIGDLATSFNDMTSRLRSSFI
                     DLERRAKEITTISEVSRRLSSVLDKQRLLIEVVEQIKSSLDYYHVHIYLLDKDSGDLI
                     MSGGTGEVGQTMLERKHRISKGRGLVGRAAESGAPVLVPDTLKDASWLPNPLLPETAS
                     EIAVPILSETGVVGVLDVQNNIQNSLSERDVELLQSIAYQVSIALKNADTYMEAQSQA
                     ERENIINEINRKIQNTSSVEQALQVAIRELGSTLGAKDSRIVLHLPDSVTKESR"
     gene            1941..>3669
                     /locus_tag="PKI33_00910"
     CDS             1941..>3669
                     /locus_tag="PKI33_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM35585.1"
                     /translation="MNTPNPSDDAMKKRIDEMFSGQELPSYASLGEVNAMKARIRELE
                     EKLYQSTKPAVASLKLEEVNSVAEEKVTEKRPKSGILSLLTPPSFGNLAKDRIAKLQH
                     QILLGIFTTAILALIAIFLNWTGPRSLFGVGILGFEFVILTFSFYFLRRGNLQVVSWI
                     LIGLIYSILISSLFVSGFTLATVIELALIVTMAGLLLHPWQVVIVSISAIVMNFVGPN
                     IGVPTTLPENNLIFTGIILALEGALLTIASSTLENTFKEIDRSTKALVISNKNLQDIS
                     ANLEERVASRTYDLELATEVGKILSGKVDNLYGLLEDAVETIRARFNLYYTQVYITDP
                     SGRTITLRAGTGDVGKQLLKRGHHLLINPSSLNGRAASEKRTVIVSNTTDNPNFLPNP
                     LLPFTRSEMAVPLIAGDRVVGVLDMQSDQANFLTENNADAFEALAGQLAIAIQNAALF
                     EQVKLSRLELEAQTKKQSYENWQNFLNAIEREEKLGYVFDQSDVLPLIKAQGIEQTST
                     ALTAPIQVAGADIGNIQFIDNAERVWTPAEEDIVQNVVSQLSVHMDTLRLLAESDKYR
                     QDAELSTKRL"
     assembly_gap    3670..3769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3770..>6723
                     /locus_tag="PKI33_00915"
     CDS             <3770..>6723
                     /locus_tag="PKI33_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM35586.1"
                     /translation="LAESDKYRQDAELSTKRLTVEGWNEYLKSRQNTTEGFIYNDHKV
                     LPLNGADIKTIGKEAVIPLQVQNQSIGEIAIETTEEFSDEELDILSNVSRQLSNHIEN
                     LRLFEQSNDSRKALEVTLQQLSTNEARLSEALDIARLGNWEYDVEKDIFTFNDHFYAV
                     FRTNVQEVGSYQMPSAEYAERFVFPEDAPLVGIEIGKAMTTTERHFKQHLEHRIIFPD
                     GSLGYISVNINVERDENGKVLRWYGANQDITDRKMAERAVYEREEQFRNTLNNIPTAI
                     VVTRMSDGNVLYANEALASMFGAPTQELIGKRSPDFYYNTNDRQRVVDIVRKDGYVKN
                     NELLFKRITGEQFWALFNVYPLKFFGENALMGSFYDLTERKEAEEAVRKAQEQYTLAV
                     DGSNDGLWDWNIETNEVYFSPRWKSMVGYEENELMNGFADFEALLHPDDHDRVLGVVN
                     DYLTGKIDDYNVEFRFRHKNGSYRWILARGKAMRNSDGAPYRMAGSHTEITERKEAEE
                     LIRTNEARLAEALEIARLGNWEYDVENDIFTFNDRFYSVFRTNIQEVGSYQLSSAEYA
                     QRFVHPEDLPIVGVEIEKALTSKDRFYAASLEHRIVFPDATTGYISVNINVERDENGK
                     ITRYYGANQDITERKQSEELIAKRAEQLSVLNRVMEVANTSQDKYYILQTATDEFRSL
                     MQGFSAGVLLLDESGDMLTLVTESYADPSMPKLAGRSMPISVNPATNSSIKSGKTVLV
                     ADAQNDPILEPIRAIMKQRNIQSVLVTPMFSRNKVIGVFSIDTNDPNRSFDNDDITLI
                     ETLAKQLASAIENVQLFDEIQHRAADLSTVATVSTTTATVLDPDELLKTVVNISKERF
                     GLYHAHIFLNNDDELVLTSGAGEVGQKMLESGLTIDINAEKSLVARAARGRQAVIIND
                     VTKEEGFLPNPLLPNTQSEMAVPMLVGESVLGVFDIQSDKINAFTEEAADIYTTLASQ
                     VAVALQNARLYQ"
BASE COUNT         1982 a         1508 c         1451 g         1682 t
ORIGIN      
        1 cgggcgcgct ttgaataccc aggcgaaagt gaaaatcaat accacaggta aaggataaaa
       61 gaaaatgaaa ccattaaaaa tatccctatc tgtaaaatta gtgtttgctt tcttgttctt
      121 gtcactcatc ccattgggta tcgttatcta cttcaatgca gtttttggta gcgaacattt
      181 acaagaagat gccgatagac aattaggaag caccgcaaaa ctagctgcga acaatatcaa
      241 tgtatttctc actggcggct tagatcatat tcaatctaca gcggctcttg ccgaatttga
      301 agaatactta tcattaccgg cttcagagcg aaagggaagt gagatagaac aagaggttta
      361 tcaaaacctt caagctaccg tgttgttaaa tccagcttat atcacttcgg tcggagtcat
      421 ggatctaaat ggcgtcaact tggttgatac aaacccaacg caaattggcg aacgagaaaa
      481 cgaaaaagaa ttctttattc agaccataga gacaaaggcg ccatacgcct ctaatgccga
      541 gcttgatgaa ggcgagtatt cgatctattt tagcgccccg attttcgatg acaataacaa
      601 aacccttggc attgttcgaa ttcgctatga tgccaatgtc ttacaagata tcttacttaa
      661 aacagctgaa gactcaagta tagaaggggt tgtcattgac ttattcgatg aaaatcatgt
      721 ggcattggct gtaacggatg ctccagaaga tattctcaaa atacttgttc ttccttctga
      781 tgaaaagctg aaagaactgc aggaagctgg gcgtattttg caaggttctg cagaagaagt
      841 agcattaaat gacccagatc tagagacgca cttaagaaac gcagaacaaa ctcccttttt
      901 tacgattgaa tcagagggag aagaagctgc aactgctttt attcaaagca aagaatggat
      961 tgttctagcc gggcaagctc aatctattta tcaggcacca cttcaagaac agctacgcat
     1021 tgagatcacc attggtcttg cattgctagt catcgtttca gcccttgcac tattgatggc
     1081 aagaagcatt agccgcccca ttattgacct ttctggggta gcaaaagata ttgcaaatgg
     1141 gaatatagat ttacaagcaa aagtccaaac aaccgatgaa attggagatc tggcaaccag
     1201 cttcaatgat atgacctcaa ggttacgatc gagtttcatt gatttggaac gccgcgccaa
     1261 ggaaattacc accatttccg aagtgagccg ccggttatct tccgtgttgg ataagcaacg
     1321 cctcttaata gaggtcgtag aacagatcaa atcctccctg gattactacc atgtccacat
     1381 ttaccttttg gataaggaca gcggcgactt gatcatgtct ggcggaaccg gggaagttgg
     1441 gcaaactatg ttggaacgaa aacataggat cagcaaaggg cgcggcttgg tcgggcgtgc
     1501 cgcagaatca ggcgcccctg ttctggtgcc agacacgcta aaagatgcaa gctggttacc
     1561 taacccgctt ttgccagaaa ctgcctctga aattgcggtt ccaatccttt cagaaactgg
     1621 agtagttggg gttttggatg tgcaaaacaa tatccagaac agcctatccg aaagagatgt
     1681 ggagctgtta cagtctattg cttaccaagt atcgattgca ttgaaaaatg cagacacata
     1741 tatggaagcc caaagccagg cagagcgcga aaacatcatc aacgaaatca accgaaagat
     1801 tcaaaatact tcttcggtag agcaagcctt gcaagtagcc attcgcgaac taggttccac
     1861 attgggagca aaagattcgc ggatagtgtt gcacctgcct gattctgtta cgaaagaaag
     1921 ccgttagaag gagaaccttt atgaatactc caaatccatc tgatgacgcc atgaaaaaac
     1981 gtattgacga aatgttctct gggcaagagt taccttccta tgccagccta ggggaagtaa
     2041 atgccatgaa agctcgcatt cgtgaattgg aagaaaaact gtaccaatcc acaaagcctg
     2101 ctgtagcttc actgaagtta gaagaagtta attctgtggc agaagaaaaa gtgactgaaa
     2161 aacgtccaaa gtctggcatc ttaagcctac taaccccgcc aagttttggt aatctggcga
     2221 aagaccgtat tgccaagtta cagcaccaaa ttttgctggg tatctttaca acagcaatct
     2281 tggcgctcat cgctattttt ctaaactgga ctggtccacg ttctttattt ggcgtaggta
     2341 ttctgggatt tgaatttgta attctaacct tttcctttta tttcttacgc cgcggcaatc
     2401 ttcaagtggt gagttggata ttaattggtc ttatctattc catcttgatt tccagtctct
     2461 ttgtcagcgg gtttacactg gcaaccgtaa tcgagctggc gctgatcgtt accatggcag
     2521 gtttgcttct tcatccatgg caagtggtta ttgtgtctat cagtgctatt gtgatgaact
     2581 ttgtgggtcc gaatatcggt gttccaacca cattgccgga aaataatctt atcttcaccg
     2641 gcatcattct agcgttggaa ggtgctttat taacaattgc atctagcacc ctggaaaaca
     2701 cattcaaaga aattgacaga agtacaaaag cgttagttat cagtaataaa aacttacaag
     2761 atatttctgc caaccttgaa gaacgcgtag caagccgtac ttatgacctt gaattagcta
     2821 cggaagtcgg taagatccta tctggcaagg tagataactt atatggctta ttggaagatg
     2881 cggtggaaac aatccgcgct cgtttcaatt tgtactatac ccaagtttat attacagatc
     2941 cttctgggcg caccatcacc ctccgtgcag gaactggaga tgtaggcaag caattgctta
     3001 agcgtggtca ccaccttttg atcaacccta gctcattgaa tggacgtgcc gcttctgaaa
     3061 aacggaccgt tatcgtttcg aacacaaccg ataatcccaa tttcctcccc aaccccttac
     3121 tccccttcac ccggtctgaa atggccgttc ctttgattgc aggtgatcgc gttgtaggcg
     3181 ttttggatat gcaaagcgat caagccaatt tccttaccga aaacaatgcc gatgcctttg
     3241 aagccttggc tgggcaattg gcaattgcca ttcaaaatgc agctctcttt gaacaggtca
     3301 aactatctcg tttggaactc gaagcccaaa caaagaaaca atcctatgaa aactggcaaa
     3361 acttcctgaa cgcgatcgaa cgggaagaaa agcttgggta tgtctttgac caaagtgatg
     3421 tcttaccgtt gataaaagca caaggtatag aacaaacctc aacggctcta actgccccta
     3481 ttcaagtggc tggtgcagac attggcaata tccaattcat agacaatgct gaacgggtct
     3541 ggactcctgc cgaagaggat attgtacaaa atgtagtttc ccaactttcg gttcacatgg
     3601 ataccctcag actattagca gagtctgata aatatcgaca agatgcagag ttaagtacga
     3661 aacgtctaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tagcagagtc
     3781 tgataaatat cgacaagatg cagagttaag tacgaaacgt ctaaccgtag aaggctggaa
     3841 tgaatacctt aaatcacgtc aaaacacgac ggaaggtttc atttataacg atcacaaagt
     3901 acttcctctc aacggcgcag acatcaaaac catcggcaaa gaggcggtta ttccgctcca
     3961 ggtgcaaaat caaagcattg gcgaaattgc aattgaaacg accgaagagt ttagcgatga
     4021 agaactggat atcctttcca atgtttcacg ccagttgagc aatcatattg aaaacttaag
     4081 attgttcgaa caaagcaatg acagtcgaaa agcattggaa gttacgctgc aacaattaag
     4141 caccaacgaa gcccgtcttt ctgaagcact cgatattgcc cgccttggca actgggaata
     4201 tgacgttgaa aaggatatct ttacattcaa cgaccatttc tatgctgtct tccgcacaaa
     4261 tgtacaagaa gtcggaagct atcagatgcc atctgctgag tatgccgaac gttttgtatt
     4321 ccctgaagat gctccattgg ttggtatcga aatcggaaaa gcgatgacca ccacagagcg
     4381 ccacttcaaa cagcatctgg aacatcgcat tattttccca gatggcagcc ttgggtatat
     4441 ctcggtcaat atcaacgttg aacgcgacga aaatggaaag gttcttcgct ggtatggtgc
     4501 caaccaggat atcaccgacc gcaaaatggc agaacgagca gtctatgagc gtgaagaaca
     4561 attcagaaat acgctcaaca atattccaac tgcaattgtg gttacccgca tgtctgatgg
     4621 gaatgtacta tatgcaaatg aagcattagc aagcatgttt ggcgcaccta ctcaagaact
     4681 aatcggtaaa cgatcgccag acttttatta caatacaaat gaccgtcaac gagttgtgga
     4741 cattgtgcga aaagacggtt atgtaaagaa caatgaactc ctattcaagc gtatcacggg
     4801 agaacagttc tgggcgctct ttaacgtata cccattgaaa ttctttggtg aaaatgcgct
     4861 tatgggttcg ttctacgacc ttactgagcg taaagaagca gaagaagctg tccgcaaagc
     4921 gcaggaacag tacacccttg cggtagatgg ttctaacgac ggcttatggg actggaatat
     4981 tgagacaaac gaggtgtact tctcaccacg ctggaagtcc atggtcgggt atgaagaaaa
     5041 tgaattaatg aatggctttg ctgatttcga agcccttctg caccccgatg atcatgaccg
     5101 tgtgcttggt gttgtaaacg actacctgac tggaaaaatt gatgactaca acgtcgaatt
     5161 ccgcttccgc cataaaaacg ggtcgtatcg ttggatcctc gctagaggta aagccatgcg
     5221 taacagcgat ggagctccct atcgtatggc agggtctcat acagagatta ccgaacgcaa
     5281 agaggcagaa gagctcatcc gtaccaacga agcccgcctg gctgaagcgc ttgaaattgc
     5341 ccgccttggt aactgggaat acgatgttga aaacgatatc tttactttca acgaccgctt
     5401 ctattcagtc ttccgcacaa atatacaaga agttggaagc taccaacttt catcagcaga
     5461 atatgcccaa agatttgttc atcctgagga tctacccatt gttggtgtag aaatcgaaaa
     5521 ggctcttacc tccaaagacc gtttttatgc tgcctcactt gaacatcgca ttgttttccc
     5581 agatgctaca actggctata tttctgtcaa cattaacgta gaacgcgatg agaacggaaa
     5641 aatcactcgt tactatggag ccaatcaaga tatcaccgag cgtaaacaat ctgaagaact
     5701 cattgcaaaa cgcgctgaac aactttctgt tcttaaccgt gtgatggaag tggcgaacac
     5761 ctcacaagat aagtattaca tcttgcagac cgctacagat gaattccgca gtttgatgca
     5821 aggtttcagt gctggcgtat tgttgctaga tgaaagcggc gacatgttga ccctggtcac
     5881 tgaaagctat gcggatccgt ccatgccaaa attagcaggg cgctccatgc cgatctcggt
     5941 taaccccgcc accaactctt ccatcaaatc tggcaaaacg gttctggttg cagatgctca
     6001 aaatgacccg atccttgaac cgatccgcgc cattatgaag cagagaaata tccaaagcgt
     6061 gctcgtcacc ccgatgttct cacgcaacaa ggtgatcggt gtgttctcaa tcgacaccaa
     6121 tgacccgaac cgctccttcg acaatgacga cattaccttg attgaaacgc ttgccaagca
     6181 actcgccagt gcaatcgaaa acgtccaatt gttcgatgaa attcagcatc gtgcagcaga
     6241 cctctccacc gtggcaacgg tcagtaccac cacggctacc gtgttagacc ctgacgaact
     6301 tttgaaaacc gttgtgaata tttcgaaaga acgcttcggt ctgtatcatg cccatatttt
     6361 cttgaacaac gatgacgaat tggtgctcac ttcaggggca ggcgaagtcg gtcagaaaat
     6421 gctagagagc ggtctgacga ttgacataaa cgccgagaaa tccctggttg cacgtgccgc
     6481 ccgcggaaga caagccgtca tcatcaacga cgttacgaag gaagaaggct tcctccccaa
     6541 cccattactg ccgaacacac aatctgaaat ggctgtgccg atgttggtag gtgaaagtgt
     6601 tctgggtgtg ttcgacattc aatctgacaa aatcaacgcc ttcacagaag aagccgccga
     6661 tatttacacc acacttgcct cacaggtcgc tgtagcactt caaaatgccc gcttgtatca
     6721 gga
//
LOCUS       DAOREF010000025         6705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5871_length_6705_cov_5.863459, whole genome shotgun sequence.
ACCESSION   DAOREF010000025 DAOREF010000000
VERSION     DAOREF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_6705_cov_5.863459"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..534
                     /locus_tag="PKI33_00920"
     CDS             151..534
                     /locus_tag="PKI33_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNM35587.1"
                     /translation="MPTYDFICNTCEKRFEVFLTYSEYGNKTVQCAHCDSTDVRRRMT
                     KVRIAKTDESRLEHMAGDLGSLEGMENDPAAFARKMKKMGKEMGEELPAEFDEVVDRL
                     EAGQSPEEIESAMPDLSGGDELSKE"
     gene            758..2914
                     /locus_tag="PKI33_00925"
     CDS             758..2914
                     /locus_tag="PKI33_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35588.1"
                     /translation="MSLKKFLVLLGVLVVLGAVLAACGGTQPTQAGTETATEEAPAAP
                     IPDTPYLADWQGSAHANVADEPFRHWDDAAENPDGVPTSCAKCHTTDGYRDFLGADGS
                     EAGKVDVAVAADHAQGVQCVACHNAVTMTKTTVMFPSGVEITAGDDSRCMECHQGRES
                     KVSVDAQIEKFAATDPDAVVAPIKDDQGNDVFFGFRNVHYYAAAATLYGGMTHGGYEY
                     DGLTYDAKNTHTDGYASCTGCHDPHTLEVKVDQCAFCHEDVATVEDLKNIRMVSSAPD
                     YDGDGNVEEGMYYEIEGLQAALLAEIQTYAADKGGAAIIYDSASYPYWFIDTNANGAA
                     DEGEVAFPNAYSTWTPRLLKAAYNYQVSMKDPGAFAHGNKYIVQLLFDSIADLGGDTS
                     KLARTDAGHFAGNTEPFRHWDKDEEGNPVYEVEAACAKCHSAAGLPQFLEYGANLPVE
                     SANGFMCSTCHNEEAWPERYAVASVTFPSGSTVSFGGKDADGNFVADDSNLCIECHQG
                     RSSTPTLNKALTGKEEDTPDPKIRFSNIHYFAAGATIFGADVQGAYMYEGKEYVGQNM
                     HADEAGKMNKCQDCHDVHALEPKVEACETCHDTTDPTAVRESDVDYDGDGDVTEGIKG
                     EIDTMTEALYAAIQAYAEGHGGAIEYKATAYPYFFGADGKGYAAWTPRLLKAAFNYQY
                     VQKDPGAYVHNPKFVIQFLYDSIQDLGGDVSAFTRP"
     gene            3108..4274
                     /locus_tag="PKI33_00930"
     CDS             3108..4274
                     /locus_tag="PKI33_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNM35589.1"
                     /translation="MHQRIKLRTPHIWTVSLLLGLGLFAFALAALTTTAQAAPSLQPA
                     AQDQKPNNDFCLACHQEEGIDKTLGSESLPVTINPTEFGLSVHAEEGVLCVDCHVDIS
                     DYPHPDVKSKSVRDFQMTFADSCKECHEDQFTDAHDSVHQTAMDEGDPNAPLCADCHN
                     PHTQGRIIGETSGELTIAARKEVPATCAKCHTETVEIYKNSVHGSALADGNTDVPTCT
                     DCHGVHNIGDPTTASFHNNTPALCADCHDNEELMGKYGLSTEVLETYVADFHGTTTTL
                     FEQQFPGQPINTAVCTDCHGVHDIASTKNPETGIAMQENLLVKCQRCHPDATANFSAA
                     WMGHYQPSPEHYPIVYYVNLFYKFFIPAVLGGMVLFILTDIYRRIINRAKGAKH"
     gene            4274..5128
                     /locus_tag="PKI33_00935"
     CDS             4274..5128
                     /locus_tag="PKI33_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNM35590.1"
                     /translation="MNTVTKAYLRFPIARRIEHWVMMLSFTLLGLTGLPQRFPEAGIS
                     QAILGALGGIQGVRTIHHTSAIVMMFGTAWHILVMGYSVFVLRDKMSMLPTLQDAKDG
                     LQALLYNIGFAKTYPQMGRYTFEEKMEYWAFVWGALVMGATGFMMWNPITATKFLPGE
                     FVPAAKAAHGNEAVLAVLAIIIWHMYGVHIKRLNKAMFNGMQTEAEMLHEHPLELADI
                     KAGIADRRPDAATLRKRQMIYYPIAAVLTIGMLSGIYGFINAEETALTTIPPIVETVE
                     PPAPTVQP"
     gene            complement(5190..6455)
                     /gene="nrfD"
                     /locus_tag="PKI33_00940"
     CDS             complement(5190..6455)
                     /gene="nrfD"
                     /locus_tag="PKI33_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNM35591.1"
                     /translation="MKTILKTIRENIFTPWGLWLAFLGLLLVSALVAALTVFWKGLVI
                     TNLTNLVPWGLWITIDLSSIAVAAGAFSLCAIVYLFGFKRYEPIARTATFIGFTGYSM
                     AMMALLLDIGRPERFWKSFVFWNPHSLLWEVTMCVGLYFTVLIFEVLPIVASFEWLRT
                     KYPKIAEKMEHMHHYAPVLAVLGLALSSLHQSSLGAVYGVIKARPIWYRPEMSVMFMF
                     TAILGGVSLTLLASMLAARITPKAKVNDTLIERVAQVLGYMMIAYLYWRFWDWLAQTY
                     TYEPGRTEGFRLLTKGPLSFNFWFGEMLFGALFPTIILLKRSLRQTPLWRMLAFAMIV
                     GGVVAFRFDTNIVGFLAVIPYTPGEMIVQYTSYTPSLVEWAAGLGIIAFGLLAFSVGV
                     KYLRVVDHRLVSEEYEVITMKVGEAVTGD"
     gene            complement(6457..>6705)
                     /locus_tag="PKI33_00945"
     CDS             complement(6457..>6705)
                     /locus_tag="PKI33_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35592.1"
                     /translation="FCYQRIDRGLSLGLKPGEDPAATPACVVACPTGARIFGDLNDPN
                     SNVSQLLKDHPSYRLRENLGTKPRVYYLPVNDDTTVEG"
BASE COUNT         1727 a         1773 c         1664 g         1541 t
ORIGIN      
        1 tgcgttgcac cacagagtct ttgcctacac cagaaggacc agagatgacg atcaacaagg
       61 gattgggttt acgaagctcg aattcactca tccaagtatt gtaccaaacg cctcttgccg
      121 accttgggct tgattctgta aaataaacat atgcctactt acgactttat ctgcaacacc
      181 tgtgaaaagc gctttgaagt ttttttgact tatagcgaat acggaaataa aacggttcaa
      241 tgtgcccact gtgacagcac cgacgtacgt cgccgcatga ccaaggtccg cattgcaaaa
      301 acggacgaaa gcaggctgga acacatggca ggcgacttgg gtagtttgga agggatggaa
      361 aatgaccctg ccgcatttgc tcgtaagatg aaaaagatgg ggaaagagat gggcgaagag
      421 ttgccagccg agtttgacga agtggtggat aggctggaag ctgggcaaag ccccgaagaa
      481 atcgaatcag ccatgccaga ccttagtggg ggcgacgagc tttctaagga ataaaccctt
      541 tccccgacgt ataatttcac taatcttatc taaattgttg tgacttttgt cacattaagg
      601 gatgcttatt tttactgtgc attaatctcc cacttaagta aaatttctca tatctgctaa
      661 aaaatgtcaa agcagtcatt ttctccaaag gaggcatatt ttcaagaaca cgccgatatt
      721 taccagcttt tttacactga tctaaggaga atgaataatg agtctcaaaa aatttctagt
      781 tttgctcggg gttttggttg tgctcggcgc ggttttagcg gcctgtggcg gcacccaacc
      841 cactcaagca ggtactgaaa cagccacgga agaagctccc gctgctccaa taccagatac
      901 tccatactta gctgattggc aaggctctgc tcacgcgaat gtagcagacg aacctttccg
      961 tcactgggat gacgctgccg aaaatcctga cggcgttccc acatcctgcg ctaagtgcca
     1021 taccaccgat ggttatcgtg atttcctcgg tgccgatggt tccgaagccg gtaaagtgga
     1081 tgtagccgtt gctgccgacc atgcccaggg cgtgcagtgc gtggcttgtc acaacgctgt
     1141 aaccatgacc aagaccacgg tcatgttccc ctccggtgtt gaaatcacag ccggtgatga
     1201 tagccgttgt atggaatgtc atcagggacg tgagtccaag gtcagcgtgg atgcgcagat
     1261 tgaaaagttt gccgccaccg accccgatgc cgttgttgct ccgatcaagg atgaccaggg
     1321 caatgatgtg ttctttggtt tccgcaatgt tcactactat gctgcagctg ccaccctcta
     1381 cggtggtatg acccatggtg ggtatgaata tgatggcttg acctacgatg ccaagaacac
     1441 ccacactgat ggttatgctt cctgcacagg ttgtcatgat cctcataccc tcgaagtcaa
     1501 agttgaccag tgcgctttct gtcacgaaga tgtagccacc gtggaagatc tcaagaacat
     1561 ccgcatggtt tcatccgctc ctgactatga tggcgatggc aatgttgaag aaggtatgta
     1621 ttatgagatc gaaggcttac aagctgcttt gttggctgag atccaaacat atgccgctga
     1681 taagggtggc gctgcgatca tctacgattc agcatcctat ccctattggt ttatcgacac
     1741 aaatgccaat ggcgcagccg atgaaggtga agttgccttc ccgaatgcgt attccacctg
     1801 gactcctcgt ttgctcaagg ctgcttataa ctatcaggtc tctatgaagg atcctggtgc
     1861 gtttgcccac ggcaacaagt acatcgttca gttgctcttc gactcgatcg ccgacctcgg
     1921 tggcgatacc agcaaactcg ctcgtaccga tgcgggtcac ttcgctggca acaccgaacc
     1981 cttccgccat tgggacaagg atgaagaagg taatcctgtc tatgaagtag aagccgcttg
     2041 tgccaagtgc catagcgctg ctggtctgcc gcaattcctt gaatacggcg ccaacctgcc
     2101 tgtggaatct gccaacggtt tcatgtgctc cacctgtcac aatgaagaag cctggcccga
     2161 acgctacgcc gttgcttctg tgaccttccc cagcggttca accgtcagct ttggtggtaa
     2221 agatgccgat ggcaacttcg tcgccgacga ctccaacctt tgtatcgaat gccatcaagg
     2281 tcgctcttct acaccgactt tgaacaaggc actcactggc aaggaagaag atactcctga
     2341 tccaaagatc cgcttcagca atattcacta ctttgctgcc ggcgctacca tctttggtgc
     2401 tgatgttcag ggcgcttaca tgtacgaagg caaggaatat gtaggtcaga acatgcatgc
     2461 tgacgaagct ggcaagatga acaaatgcca ggattgtcac gatgttcacg ctctcgaacc
     2521 caaagtcgaa gcctgtgaaa cctgtcacga caccaccgac ccaacagcag tccgcgaaag
     2581 cgatgtggac tatgatggcg acggcgatgt aaccgaaggt atcaagggcg agatcgacac
     2641 catgaccgaa gccctctatg ctgcaattca ggcatatgct gaaggacatg gcggcgccat
     2701 tgaatacaaa gccactgctt acccgtactt cttcggtgcg gatggcaagg gatatgcagc
     2761 gtggactcct cgcttgctca aggctgcctt caactaccag tacgtccaga aagacccggg
     2821 tgcgtacgtt cacaatccca agttcgttat ccagttcctg tatgactcca ttcaggacct
     2881 gggcggtgat gtgagcgcgt tcacacgccc gtaagctcca agtaataaaa aatggcggat
     2941 ggcaacatcc gccattttct ttttaatcca attcttaatc acaccaaaac cgtgatattt
     3001 agcactgtaa tggggcgcat gccccactta caattggggc aattccccca cgtttgtaat
     3061 cgggaatata atatgtaaat tgtccgaatt ttaaaaagga gaatgtgatg caccaaagga
     3121 tcaaacttcg cacgcctcac atctggaccg tgagcctgct tctcggactt gggcttttcg
     3181 ccttcgctct agcagcactc accacaaccg cgcaagccgc cccctccctt cagcccgcag
     3241 cacaagacca aaaaccgaat aacgattttt gtcttgcctg tcaccaagag gaaggcatcg
     3301 acaaaaccct cggaagcgaa tcacttccag taaccatcaa ccccactgaa tttggtttat
     3361 cggttcacgc tgaagaaggc gttctctgtg tagattgcca tgtagatatc agcgactatc
     3421 cccacccaga cgtaaaatca aaatccgttc gtgattttca aatgaccttc gccgactcct
     3481 gcaaagaatg tcacgaagat cagtttaccg atgcgcatga tagtgtccat caaaccgcga
     3541 tggatgaagg cgaccccaac gccccactct gcgccgactg ccacaacccc cacactcagg
     3601 gacgcatcat tggcgaaacc agcggcgagc tgaccattgc cgcccgcaaa gaagtgcctg
     3661 ccacttgtgc caagtgccat accgaaactg ttgaaattta caagaatagt gttcatggca
     3721 gcgccctggc agatggcaat acggatgtgc ccacctgtac agattgtcac ggtgtgcaca
     3781 acatcggtga ccccaccact gcctctttcc acaacaacac acctgccttg tgcgcagact
     3841 gtcacgataa cgaagagttg atgggcaaat acggtctttc caccgaagtg cttgaaacct
     3901 acgtggcaga tttccacggc accaccacca cactctttga acagcaattc cctggtcaac
     3961 cgatcaacac cgccgtctgt acagattgcc acggtgttca cgacatcgcc agcaccaaga
     4021 accctgaaac gggtattgcc atgcaggaga atctgctcgt caagtgccag cgctgtcacc
     4081 ccgatgcaac tgctaatttc tcagccgcat ggatgggaca ctaccaaccc agccctgagc
     4141 attacccgat cgtctattac gtcaacctct tctataaatt cttcattccc gcagtgttgg
     4201 gtggtatggt gctctttatc ctcaccgata tttatcgccg catcatcaac cgcgcgaaag
     4261 gagcaaagca ctaatgaata ctgtaaccaa agcttacctg cgcttcccga tcgcgcgccg
     4321 tattgaacac tgggtgatga tgctctcttt caccttgctc ggtctcactg ggcttcctca
     4381 gcgctttcca gaggctggaa tttcacaagc cattctcggt gccttgggcg gcattcaggg
     4441 tgtgcgcacg attcatcaca cgtctgctat tgtcatgatg tttggcaccg cctggcatat
     4501 tctcgtaatg ggttacagcg tcttcgtctt gcgcgataag atgtccatgc tgcctacatt
     4561 acaagatgcc aaagatggtt tgcaggcatt gctctacaac attggctttg ccaaaaccta
     4621 cccacaaatg gggcgctaca ccttcgaaga gaagatggaa tactgggcgt tcgtttgggg
     4681 cgcattggtc atgggcgcca ctggtttcat gatgtggaat ccaattacag caacaaagtt
     4741 cctgcccggc gaatttgtac cggctgccaa agctgctcat ggtaacgaag ctgtgttggc
     4801 tgtgcttgct atcatcatct ggcacatgta tggcgtgcac atcaaacgcc tgaataaagc
     4861 catgttcaac ggtatgcaaa ccgaagccga aatgctgcat gaacatccgc ttgagttggc
     4921 agacatcaaa gctgggattg cagatcgccg ccccgatgcc gcaactctgc gcaagcgcca
     4981 gatgatctac tatcccatcg ccgctgtctt aaccatcggc atgctcagtg gaatctatgg
     5041 ctttatcaac gctgaagaaa ccgcgctgac gaccattccg cccattgtgg agacggtcga
     5101 accacctgca ccaacggttc aaccatagga acaagaatca aaaaagagcc gcccttagag
     5161 gcggctcttt tttgattcta taccgaacct taatctcccg taacggcttc gccaaccttc
     5221 atggtgataa cttcatattc ttcactcaca aggcgatgat ctacaacgcg taagtatttc
     5281 acaccgacgg agaaagccag cagaccaaag gcgatgattc ccaacccggc tgcccattct
     5341 acgagcgaag gagtgtagct ggtgtactgg acgatcatct caccgggggt atacggaatg
     5401 accgcaagga aaccaacgat attcgtatcg aagcggaagg ctactacgcc gccaacgatc
     5461 atcgcgaagg cgagcatccg ccacagcgga gtctgacgca gggagcgctt gagcagaata
     5521 atggtaggaa agagcgcgcc gaagagcatc tcgccgaacc agaagttgaa ggagagcggt
     5581 cctttggtga ggagtctgaa tccttcggtg cgtccgggtt cgtaggtgta ggtttgggca
     5641 agccaatccc agaagcgcca gtagaggtaa gcgatcatca tatacccaag cacttgagcg
     5701 acacgctcga tcaatgtatc gttgaccttg gctttgggcg taatgcgtgc tgccaacatg
     5761 gatgccaaca gtgtgagcga tacgccgccg aggatggctg tgaacatgaa catcacagac
     5821 atttcagggc gataccaaat ggggcgagct ttgatgacgc catatacagc accgagtgaa
     5881 gattgatgca aactggagag cgccaagcct aagacggcaa gcacaggtgc gtagtgatgc
     5941 atgtgttcca tcttttcggc gatcttcggg tatttggtgc gtaaccattc aaaggatgcg
     6001 acgatgggca agacttcgaa gatcaatacc gtgaagtata gacccacaca catggttact
     6061 tcccaaagca gagagtgagg attccagaag acaaaggatt tccagaaccg ttcagggcga
     6121 ccaatatcca gcaatagcgc catcattgcc atgctgtagc ccgtgaagcc aatgaaggtc
     6181 gccgtgcggg cgataggttc atagcgtttg aagccgaaga ggtacacaat tgcgcacaaa
     6241 ctgaaggcac cagccgccac ggcaatggac gagagatcga tggtgatcca caaaccccaa
     6301 gggactaggt tggtgagatt ggtgatgacc aagcctttcc agaagacggt gagtgcggcg
     6361 accaatgcgc tgaccaacag aagccctaga aaagcgagcc ataaccccca aggggtaaag
     6421 atattttctc gaatggtttt taggatggtt ttcatgtcat ccctccacag tggtgtcgtc
     6481 atttacgggc aggtaataga cccgcggttt ggttccaaga ttttcgcgca agcgatagga
     6541 cggatgatcc ttcaagagct ggctcacatt ggaattcggg tcgttcaagt caccaaagat
     6601 gcgggcgccg gttgggcagg caactacaca agctggggtg gcggcggggt cttcaccggg
     6661 tttaagcccc agtgaaagtc cgcggtcgat gcgttgatag cagaa
//
LOCUS       DAOREF010000026         6671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5916_length_6671_cov_4.550030, whole genome shotgun sequence.
ACCESSION   DAOREF010000026 DAOREF010000000
VERSION     DAOREF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_6671_cov_4.550030"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1219)
                     /locus_tag="PKI33_00950"
     CDS             complement(206..1219)
                     /locus_tag="PKI33_00950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM35593.1"
                     /translation="MDYTLLNDFSQIQAQEWNDLLKESISDTPFLRYEYQQAWWQHRG
                     GGEWKNAQLILVTARENGKLIGIAPLFIAEYENQPALLLNGSIEISDYLDLIVRKEDH
                     TNFLTGLLDFLASSLADNWSKLDWYNIPDDSPTLAALKAEGEKRGWAHLEEVYRPTPR
                     IALNGNFEDYLANIDKKQRHEIRRKMRRAAESELNVRFVIVDQSADIEPELRAFFDLM
                     VQDSNKATFLHPAMREQMTAFLRAAHAGGYLWLGFLEIAGEKTAASLNFDYNGKLWGY
                     NSGVSRSNMELSPGWVLMAHTIQWCCENGRYEFDFMRGDEDYKYRLGGVNRFVMRARL
                     NRK"
     gene            1272..3764
                     /locus_tag="PKI33_00955"
     CDS             1272..3764
                     /locus_tag="PKI33_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35594.1"
                     /translation="MPLDINLSPLYRVQGQEPTEMPGVLALQPPKNAARGREQDRLIV
                     YLALTGNATITTTEYRKLAEDVANVFYQTPRAVTSALRAASDSLNKVLLDRNMSSSNR
                     GQYTLAWLALAAVRESQSIFSLCGPMHAYWFSANGTRHFFEPTISGKGLGSSQTINIH
                     YAQSDLSPNDMLLFCGRLPNAWVTPLEDAQPSSFDAMRRRLTSITNEDLNAVLIQTTT
                     GAGVINLLRGNMVTKTEAPVEVAPTEIITEAPVQEQPQAIEEPNLEEEPAPQTSTPDL
                     PATYEPASTPEGGVHLLQPSTYSLPPQREETAFPASIPNADPLANLPHRTGPREFPAS
                     IPRAKPRTEAEAPAIEENPIVEKIEPKPEPVQEIKTEKESVPEIKIPEGPREPSPQAK
                     QAAKIVVNLMQGFRTSSASLGERFRNFLPRLLPAEEQSTTSVSANAFMGFMAVLIPLI
                     VVTLAVVVYLRYGRNEQYEAYYNQALQMKQQALALTNPVEQRIAWENVLANLDQAENH
                     NVTSDTMNLRQEAESKRDQLLGISRMQFNPAFTAKLGIDVSRMAATENDLYLLNAANG
                     EVLRAIPAGNGGFELDNTFNCTPGVGNTVGPLVDILALPITNIYSATVLGIDASGNLL
                     YCKPGATPQTGLLPAPDTNWGRITSFILDSGNLYVLDAPSRAVWVYLGKESAFVDRPY
                     FFFGQQTPTQDVIDFLVIGDEMYLLHADGRISNCTYSRVDTNASKCQDPVNKINPFPG
                     YADMDLFATAHFTQILFAAPPDPSLLLLDADNQVVMRFAPRTLELQNQFHPTSGRSNP
                     IPRSPVSAVAVSPDHVLYLAVNGQVYFAPNMP"
     gene            3783..4763
                     /locus_tag="PKI33_00960"
     CDS             3783..4763
                     /locus_tag="PKI33_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35595.1"
                     /translation="MPSVLETIANLFRPAPYPKDNTTEPAQIIRSKVLVIVYDPVMDA
                     AGTKLSKYMKWKDTTDLISGFMLDMLDVTEGKVRYEVVERIDVNEFPAKTDGFRYTAQ
                     TYLDVWNRTSAPHMPQEVNYSAILAQFNILNRVARSEIDEVWIFGFPYGGFYESTMAG
                     PGAFWCNAPPLANTALSKRRFVIMGFSYERGIGEMFESYGHRAESIMEKTFSKLTGEA
                     NLWKRFIRYDKVAPGKAACGNVHFAPNSQADYDWGNKTPVKSECYDWLLNFPNFKGDI
                     RTVDSYEWGGGEIRAHHKWWFNHFPRVAGRKNGIHNNWWQYIAAPQNVII"
     gene            4885..>6671
                     /locus_tag="PKI33_00965"
     CDS             4885..>6671
                     /locus_tag="PKI33_00965"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNM35596.1"
                     /translation="MYNADFLVPFVYILAAIFYALVGLYAWRKRPAVGVVSFAWVMLS
                     MSIWSLTYALEIFLPTTAAKLQVLNIEYLGILGVPVFLFFFALDFTGRSHLLTPRFRL
                     ILWGVPAFILLMVWTNPLHSLMWGKVETFRSGSLSLLSVDFRSAFWVNIIYTYGLVIA
                     ACIILIMEFLQRPGSLRLQISFVILSLLASLLGSSTFVLNISPIVGVDLSPLFFLPAA
                     IGLAWVTLRYRLAEILSLEHITVLQNMKDSVIVLNETKKVIYINQVTEKLFNLTEDKV
                     IGQPFNFVSGRLANLLDPYISPNEQHHNEIEIGDGEEKKTYEVSISPIVSATRNTSEI
                     IIALHDITLRKEKEQELSRHGAIMSAVSRAAEQFLKTEDWEQNISNVLENLGQAADVS
                     RVSVRIEPVDNATSVKNATYEWKAQENPYSEKPLSQVVFPVFVNGIQWATISFDEFRN
                     DRQWSNMELEAFQTAANILAAAETRTQTVHQLNRRQLALGLLQDIATVSLQAANLNEM
                     AEVSAERLAKLIKADGCYLTLWNDVTKHVIPVAAYGTGKDTYARTSAKEGEKTFTQSS
                     LELEKTLIVEDVYNTPYASASVVSRFPAKS"
BASE COUNT         1795 a         1630 c         1490 g         1756 t
ORIGIN      
        1 tcttcttttt caatggattc tttcaagcgt ttttcaacat caggcggcaa agagctggat
       61 gtgcgccgaa tgagttctaa aatttcgttg gttaaatctc gcatgggtac ctccaataca
      121 gaattgaatc ttactcccga aaaggggaat ttgccacata aaaacggagc cctgcgtatt
      181 ttctaacgct ggactccgta aaaaattact ttctattaag tctggctcgc atcacaaagc
      241 gatttacgcc gccgaggcgg tatttgtaat cttcatcgcc gcgcatgaag tcgaattcat
      301 aacgtccgtt ttcgcaacac cattgaatgg tatgagccat caacacccag cctggcgaaa
      361 gctccatatt cgaacggcta acaccagagt tgtagcccca gagttttccg ttgtaatcaa
      421 agttcaaaga agcagcggtc ttttcaccgg caatctctaa gaagccaagc cagagatagc
      481 cacctgcatg agcagcgcgc aagaaggcgg tcatttgctc gcgcatggca gggtgtaaaa
      541 aggttgcttt gtttgagtct tgcaccatca ggtcgaaaaa ggcgcgaagt tcaggctcga
      601 tatctgcaga ttgatctaca atgacaaagc gaacattgag ctcagactca gccgcgcggc
      661 gcatcttgcg acgaatttca tggcgctgtt ttttgtcgat gttggcgaga taatcttcaa
      721 agttgccgtt gagggcaatg cgtggagtag ggcgatacac ttcttcaaga tgagcccagc
      781 cgcgtttttc gccttcggct ttgagggcgg cgagagtagg cgaatcatca gggatgttat
      841 accaatcgag tttggaccag ttatcggcta gggaagaggc gaggaagtct aatagccccg
      901 tgagaaaatt tgtatggtcc tctttgcgga cgatgagatc cagatagtcc gaaatttcaa
      961 tgctgccatt gaggagcaaa gcaggctggt tttcatattc tgcaatgaaa agcggagcaa
     1021 tgccaatgag tttgccattt tcacgagctg tgacgagaat cagttgagca ttcttccact
     1081 caccaccacc acggtgttgc caccatgcct gttggtattc gtatcgaaga aagggcgtgt
     1141 cgctaatgga ctctttgaga aggtcgttcc attcttgcgc ctgaatttga gagaaatcgt
     1201 tgagaagtgt atagtccata gccgtcgaat tttaccagtt ataatgaagg cgacttaagc
     1261 gattatctta catgccactc gacatcaatc tttcaccttt atatcgtgtt caggggcaag
     1321 aacccactga aatgcccggc gtactggcgc tacaaccacc caaaaacgcc gctcgtgggc
     1381 gcgaacaaga ccgactgatc gtatatttgg cgctcaccgg caatgccacc atcactacaa
     1441 ctgaatatcg caaactcgct gaagatgttg cgaatgtgtt ctatcaaact ccgcgtgccg
     1501 taaccagtgc tttgcgcgcc gcttctgatt cgttgaataa agtgttgctc gaccgtaata
     1561 tgagctctag caatcgtggt caatacacgt tggcatggtt ggctctcgcg gctgtgcgag
     1621 aaagtcagag catcttttcg ttgtgtggtc ccatgcatgc gtattggttt agcgcaaatg
     1681 gcacgcgtca tttctttgaa cctacaattt ctggcaaagg tttgggctcc agccaaacta
     1741 tcaacattca ttacgcacaa agtgacctaa gcccaaacga tatgttgctt ttttgcgggc
     1801 gtctccccaa cgcttgggtg actccgcttg aggatgcgca accctcttct ttcgacgcca
     1861 tgcgccgccg cctcacttcg attaccaatg aagacctgaa tgcggtgctt attcaaacca
     1921 caactggtgc aggcgtcatc aaccttctgc gcggcaatat ggtaacaaaa acagaggcac
     1981 cggtcgaagt cgcccccaca gaaatcatta cagaagcgcc tgttcaagag caacctcaag
     2041 caatagaaga accaaatttg gaggaagaac ccgcgcctca aacttcgacg ccagacttgc
     2101 cagccaccta tgaacctgcg tcaacgcctg aaggtggcgt tcatctctta caaccttcca
     2161 cctattctct tccgcctcaa cgtgaagaga cggcattccc cgcatctatt ccaaatgcag
     2221 acccattagc gaatcttcca caccgaacag gtccacgcga atttccggca tccattccga
     2281 gagccaaacc tcgaacagaa gcagaagctc cggcaatcga agagaatcct atcgtcgaaa
     2341 aaatcgagcc gaagccagaa cctgttcaag aaatcaaaac cgaaaaagaa tccgttcctg
     2401 aaataaaaat tcctgaaggt ccgcgtgaac cttcgccaca agccaaacaa gcagcgaaga
     2461 tcgtcgtcaa cctcatgcag ggatttcgca ccagcagcgc ttcacttggt gaacgcttcc
     2521 gtaatttttt gccgcgcctt ttacccgcgg aagaacaaag caccacttcg gtttcagcca
     2581 acgcctttat gggattcatg gcagtgctca ttccgctcat tgttgtaacg cttgctgtgg
     2641 ttgtttacct gcgttatgga cgcaacgaac agtatgaagc ctattacaac caagcgttac
     2701 aaatgaagca acaagctctg gctctcacta acccagttga acaacgcatt gcctgggaaa
     2761 acgtgcttgc taatttggat caggctgaga atcacaacgt caccagcgac acgatgaatc
     2821 ttcgtcagga agctgaatcc aaacgcgacc agttgctagg catttctcgc atgcaattca
     2881 acccggcgtt cactgcgaaa cttggaattg atgtcagccg catggcagcc acagaaaatg
     2941 acctctatct gctcaatgct gcaaatggtg aagtgctgcg cgccatccct gctgggaatg
     3001 gtggctttga gttagacaat acgttcaact gtactcccgg tgttggcaat acagtaggtc
     3061 cattagtgga tattcttgct ttgcccatca ccaatattta cagcgcaacc gtcttgggta
     3121 ttgatgcgag cggcaatctt ttatattgca aaccgggcgc cactccgcaa accggtctct
     3181 tacccgcccc cgatactaac tggggacgca ttaccagctt catcttggac tctggaaatc
     3241 tgtatgtgct cgatgcgcct tcgcgtgcag tatgggtgta tcttggaaaa gaatcggcat
     3301 ttgttgaccg tccctacttc ttcttcggtc aacaaacccc tacacaagat gtgatcgatt
     3361 tcctagtcat tggcgatgag atgtatctcc tgcatgccga tggtcgcatt tcaaattgta
     3421 catatagccg cgttgatacg aacgcttcga aatgtcagga cccggttaat aagatcaacc
     3481 ccttccctgg gtatgccgat atggacttgt tcgcaacggc acactttact cagatcttat
     3541 ttgcagcgcc gccagatcca tccttgttat tattagatgc agataaccaa gttgtgatgc
     3601 gttttgcacc gcgcacgctt gaacttcaaa atcaattcca ccctacatct ggaagatcga
     3661 accctattcc gcgcagccct gtcagtgccg tagcggttag ccctgatcat gtgttgtatc
     3721 ttgcagtcaa cggtcaggtt tatttcgccc ccaacatgcc ataacgttgt aaggaggttt
     3781 ccatgcctag cgttctcgaa acaatagcca atctctttcg tcccgctccc tacccgaaag
     3841 ataacacgac cgagcctgcg caaatcatcc gcagcaaagt attggttatc gtttatgacc
     3901 cggtcatgga tgcggcaggc accaaacttt ccaagtatat gaagtggaaa gacaccacag
     3961 acctgatttc aggcttcatg ctggatatgt tggatgtcac cgagggcaaa gtccgttatg
     4021 aggttgttga acgcatagac gttaacgaat ttcccgcgaa aacagacggc ttccggtata
     4081 cggctcaaac ctacctcgat gtctggaacc gcacctctgc acctcatatg ccgcaggaag
     4141 tgaattactc cgcgattctc gcccaattca atatcttaaa ccgagtcgcg cgcagtgaaa
     4201 tagacgaagt atggatattc ggtttcccat atggtgggtt ctatgaatct accatggcag
     4261 gtcccggcgc tttttggtgc aatgcgccac cattagcaaa tacagcgttg agcaaacgtc
     4321 gcttcgtaat catgggattc tcgtacgaac gtggcattgg cgaaatgttc gagtcatatg
     4381 gtcatcgtgc cgaatcgatc atggaaaaga ccttcagcaa acttaccggc gaagcaaatc
     4441 tatggaagcg cttcatccgc tacgacaagg tcgcgccggg caaagccgcc tgcggaaacg
     4501 tccactttgc acccaatagc caggcagatt atgactgggg caacaagact cccgtcaaaa
     4561 gtgaatgtta cgactggtta ttaaacttcc caaatttcaa aggggatatc cgcactgtgg
     4621 actcttatga atggggcggc ggtgaaatcc gcgctcacca caaatggtgg tttaaccact
     4681 ttccacgagt ggctggtaga aagaatggca ttcacaataa ctggtggcaa tacattgctg
     4741 ctccacaaaa tgtgatcata taacaaaatg gcacttcgtt ctggaacgaa gtgccatttt
     4801 gtatagataa aaaccctacc caaactatca tcttattatt atgaacttta gtcagtgcta
     4861 aaattacaat agatttgtaa gggaatgtat aacgctgatt ttctagtccc gtttgtttac
     4921 atccttgctg ccatcttcta tgcgctcgta gggctatatg catggaggaa acgcccggca
     4981 gtaggtgttg tctcatttgc atgggtcatg ctctccatgt caatctggtc gcttacctat
     5041 gcgctggaaa tcttccttcc tacaaccgct gccaaattac aagtattaaa tattgaatat
     5101 ctcggaatcc ttggggttcc agttttctta ttttttttcg cattagattt cacaggcaga
     5161 agccacctac ttacgccacg ttttaggctg attctatggg gtgttcccgc ttttatactt
     5221 cttatggtgt ggacaaatcc actccactct ttaatgtggg gcaaggtcga aacctttcgt
     5281 tctggctcct taagtttatt aagcgtggac tttagaagtg cattctgggt aaatattatt
     5341 tacacatatg ggcttgttat tgcagcctgc atcattctca ttatggaatt tttgcagcgt
     5401 ccaggcagtt taaggcttca gatcagtttt gtaatcttaa gcctccttgc ttcgcttttg
     5461 ggctcttcga cgttcgttct aaatatcagt cctattgttg gcgtagatct ttcaccgcta
     5521 ttctttctgc ctgctgccat tgggcttgcc tgggtaactt tacgatatcg tctcgctgag
     5581 atcctctcac ttgaacacat cactgttctt caaaatatga aggacagcgt tattgttcta
     5641 aatgagacaa aaaaagttat ctatatcaac caagtcacag aaaagttatt caatcttact
     5701 gaagacaaag tcattgggca gcccttcaat ttcgtatcgg gaagattagc taatttatta
     5761 gatccctata tcagtcccaa cgaacagcat cataatgaga ttgaaattgg agatggtgaa
     5821 gagaaaaaaa cctatgaagt ttctatttct ccaatcgttt cagcgacacg aaatacatct
     5881 gaaatcatta tcgcattaca cgacataact ctccgtaaag aaaaagagca agaacttagt
     5941 cgtcacgggg caattatgtc tgcggtcagt agagcagctg aacaatttct caaaactgaa
     6001 gattgggaac aaaatatatc aaacgtgcta gaaaatttag gtcaagcggc agatgtcagc
     6061 cgggtgtctg tgaggattga gcctgttgat aatgcaactt ctgttaagaa tgcaacgtac
     6121 gaatggaaag cacaagaaaa tccatattct gaaaagcctt tatcgcaagt agtctttcca
     6181 gtctttgtca atggaataca atgggcaact atttcctttg atgagtttcg aaatgaccgt
     6241 caatggagca atatggaatt ggaagccttc caaacggcag caaatatcct tgccgctgca
     6301 gagacgcgta cacaaactgt tcatcaactc aatcgccgtc aacttgcttt ggggttatta
     6361 caagatatcg caaccgtatc cctccaggca gcaaacttaa atgagatggc tgaagtatca
     6421 gcagaacgcc tagcaaagtt aattaaagca gatgggtgtt acctcacctt atggaatgat
     6481 gtaacgaagc atgttattcc ggttgctgct tatgggacgg gcaaagacac atatgctcgt
     6541 acatctgcaa aagaaggtga aaagaccttt acacaatcga gccttgaatt agaaaaaacg
     6601 cttattgttg aagatgttta caacacaccc tatgcctcgg cttcggtggt tagccgtttt
     6661 ccagcaaaat c
//
LOCUS       DAOREF010000027         6643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_5955_length_6643_cov_4.687462, whole genome shotgun sequence.
ACCESSION   DAOREF010000027 DAOREF010000000
VERSION     DAOREF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5955_length_6643_cov_4.687462"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..374
                     /gene="rpsJ"
                     /locus_tag="PKI33_00970"
     CDS             66..374
                     /gene="rpsJ"
                     /locus_tag="PKI33_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNM35597.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERTGAHVVGPVPLPS
                     KKERYTIRRSSFIDKDSHEHFEIRTHNRLIDVLDPDSKTIDMLMRLNLPAGVDIEIKI
                     "
     gene            557..1189
                     /gene="rplC"
                     /locus_tag="PKI33_00975"
     CDS             557..1189
                     /gene="rplC"
                     /locus_tag="PKI33_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNM35598.1"
                     /translation="MLKGLIGRKIGMTQIFDEQGVAYAVTLIEAGPCFVTQVRTAEKE
                     GYSAVQLGFGETNPKRLTQGQVGHLKANEITPLRYLREFRAKEHGLKVGDKVSVGDAF
                     SVGERVDVIGISKGKGFAGGVKRYHFHGMNATHGTSDRNRAPGSRGSGTTPGRVYKGA
                     RGAGHMGVDRVTAQNIKVVMVDPERNLIAINGAVPGGKGSLVMIKESRKQ"
     gene            1217..1846
                     /gene="rplD"
                     /locus_tag="PKI33_00980"
     CDS             1217..1846
                     /gene="rplD"
                     /locus_tag="PKI33_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNM35599.1"
                     /translation="MKVDVLDLKGNKVREVELPANVFEAPVNVDLMHQAYTRQMANAR
                     LGTHDTKVRGEVRGGGRKPWKQKGTGRARQGSRRAAQWVGGGRIHTPHPRSYELRMPK
                     KMRLAALRSALSVKASEASLVVVEELNFDEPKTKVVAQALGNLVGSSTALVLMPAKDQ
                     NYDVVMRSASNIESAKVLLASYLNVRDILNFEKIVLPAKTIDALVAHLG"
     gene            1856..2161
                     /gene="rplW"
                     /locus_tag="PKI33_00985"
     CDS             1856..2161
                     /gene="rplW"
                     /locus_tag="PKI33_00985"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNM35600.1"
                     /translation="MSDLYNVLVRPLVTEKSNTQSSKFGHYVFVVNNNATRTAVKDAV
                     ETLFDVTVARVNIVNAPAKRGRRGKSRRMVIRRPAFKKAIVTLAEGSKPLEIFEGVQ"
     gene            2161..2997
                     /gene="rplB"
                     /locus_tag="PKI33_00990"
     CDS             2161..2997
                     /gene="rplB"
                     /locus_tag="PKI33_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNM35601.1"
                     /translation="MAVKKYKPITPGMRDMTGYTFEEITKSTPERSLLVIKKRSGGRN
                     AYGRVTVRHRGGGARRYIRVVDFKREKFGIPAKVASIEYDPNRTARLALLHYADGEKR
                     YIVAPVDLKVGDTLMSGPNAEIRSGNSLPISNIPVGTLIHNIEMKPGKGGQLVRSAGG
                     AAQLLAKEGDFAQVRMPSGEVRLIFQVCYATIGQVGNLDHSNVKLGKAGRKRHLGIRP
                     TVRGTAMSPRDHPHGGGEGRQPVGLPGPKSPWGKPTLGKKTRLNKKTDQYIVRRRSKT
                     NR"
     gene            3016..3288
                     /gene="rpsS"
                     /locus_tag="PKI33_00995"
     CDS             3016..3288
                     /gene="rpsS"
                     /locus_tag="PKI33_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNM35602.1"
                     /translation="MSRSLKKGPYIEAKLLKRIEAMNQKKEKKVVRTWSRASVIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHRLGEFAPTRTFRGHQAAEKAA"
     gene            3301..3642
                     /gene="rplV"
                     /locus_tag="PKI33_01000"
     CDS             3301..3642
                     /gene="rplV"
                     /locus_tag="PKI33_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNM35603.1"
                     /translation="MTDIQAHVRFLPQSAQKVRAVIDVVRGKSAIEALEILKFVNKRA
                     AGPVYKLVASAVANAEENFGVSRDDLFVAKITADEAPTRKWRRFGARGRFKPLLRRSS
                     HVTVILRERGA"
     gene            3652..4401
                     /gene="rpsC"
                     /locus_tag="PKI33_01005"
     CDS             3652..4401
                     /gene="rpsC"
                     /locus_tag="PKI33_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNM35604.1"
                     /translation="MGRKVHPVGFRLGINQPWGGRWFAEGSTYRQQLHQDLAIRGLLM
                     GKLSKGGAAANEKVRELRKELPDAIRDAKAGISHVDVERTPGKIRIAIHTAKPGILIG
                     RKGEGVKKIRTALEALVGKKIELDIKEITNPDLDARLVAKNIADQLERRIAYRRAMKR
                     AIQQAMKQGAEGIKILVGGRLGGAEMSRDVWLREGRVPLQTLRANLDFATDEALTTYG
                     QIGVKVWIYKGEVAPEVEEKTEATEGVYVSE"
     gene            4433..4852
                     /gene="rplP"
                     /locus_tag="PKI33_01010"
     CDS             4433..4852
                     /gene="rplP"
                     /locus_tag="PKI33_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNM35605.1"
                     /translation="MLMPKRVKWRKQMRGRMRGKALRGAEVAFGEFGLQALEPGWITA
                     RQIEAARRSIVREMKRRGKIWIRIFPAKPFTHKPPETRMGSGKGNVEYYVAVVKPGRI
                     MFEVAGLSADIAIAALRSAQYKFSIKTKVVARHAGGE"
     gene            4855..5076
                     /gene="rpmC"
                     /locus_tag="PKI33_01015"
     CDS             4855..5076
                     /gene="rpmC"
                     /locus_tag="PKI33_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNM35606.1"
                     /translation="MKAMKSAEIRKLGLEEIRTKISDARDELMKLRFQQVTGQLTDTS
                     RLNILRKDIARMETILAEMLRNAAVEGAK"
     gene            5073..5345
                     /gene="rpsQ"
                     /locus_tag="PKI33_01020"
     CDS             5073..5345
                     /gene="rpsQ"
                     /locus_tag="PKI33_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNM35607.1"
                     /translation="MNNRRRITGVVTSNKMTKTVVVEITRKYRHPLYKKVVFSSMRVK
                     AHDEIGCQIGDEVRIVESRPLSRDKRWVVESVVKREVRTADQGVGE"
     gene            5349..5720
                     /gene="rplN"
                     /locus_tag="PKI33_01025"
     CDS             5349..5720
                     /gene="rplN"
                     /locus_tag="PKI33_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNM35608.1"
                     /translation="MIQHESRLKVADNTGARELLVIHVMGGSRRKYGAIGDVVVATVK
                     AAAPQGSVKKSEVVKAVIVRCTKEWRREDGSYIRFDDNAAVILDADGENPRGTRIFGP
                     VARELRDMGYMKIVSLAPEVL"
     gene            5730..6059
                     /gene="rplX"
                     /locus_tag="PKI33_01030"
     CDS             5730..6059
                     /gene="rplX"
                     /locus_tag="PKI33_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNM35609.1"
                     /translation="MKVKIRKGDTVEVISGRLEDKGKRGEVINVILEERRVVVQGVNV
                     RTKHQKQVQAGNRQMNPGRVQFEGPIDISNVMLVCPKCSEATRVGVQRDDEGAHRVCK
                     NCQATID"
     gene            6078..6629
                     /gene="rplE"
                     /locus_tag="PKI33_01035"
     CDS             6078..6629
                     /gene="rplE"
                     /locus_tag="PKI33_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNM35610.1"
                     /translation="MNKMQERYNKEVAPALLKSFGFKNVMQVPRLEKIVVNIGLGEAL
                     DNPKALEAAVTDLTTITGQKPVQTKARKSIANFKLREGRLIGTKVTLRGPRMWAFFDR
                     LVNIALPRVRDFRGISPNAFDGRGNYTLGLKDQLVFPEIEYDKIDKLRGMEITIVTSA
                     QNDDEARALLRQLGMPFSGKKEA"
BASE COUNT         1593 a         1659 c         1880 g         1511 t
ORIGIN      
        1 cgtgaaggcg gtctcaccgt cggtgcgggt gtcgtcacca agatcattgc gtaaggcgga
       61 gagaaatggc taaacagaag attcgcatcc gcctcaaggc ttacgatcat cgcgttctcg
      121 accaatccgc caagcggatt gtcgagaccg ctgaacgcac gggcgcccat gtagtgggtc
      181 ccgtaccctt gccaagtaag aaagaacgct atacaatacg gcgctcttcg ttcattgaca
      241 aggactccca tgaacacttc gagatccgca ctcacaatcg cttgatcgat gtgttggatc
      301 ccgactcgaa gacaatcgac atgctgatgc gcttgaattt gcccgctggt gtcgatatcg
      361 aaatcaaaat ctagttgaat aacacgtaat tttggtcatt tccaaaatta cgatacgtta
      421 gagacggcgc aagagttggt ttgcgcgcga acaaagggtt cgcctcgcaa accaactgac
      481 tgtgccgcac ggagatgatg cggtcgttgc cccgactggc agtctcgtca atatcttgta
      541 aaggagaaaa ttgagtatgt tgaaagggct gataggtcga aagatcggca tgacccagat
      601 cttcgatgag caaggcgttg cctatgcggt aaccctcatc gaagctgggc catgttttgt
      661 tacccaggtc cgcacagctg aaaaagaggg atattctgca gtgcaacttg gttttggcga
      721 aacaaatccc aaacgtctca cacagggaca ggttggacat ttgaaagcca atgaaattac
      781 ccctctgcgc tacctccgcg aattccgagc caaagaacat ggtttgaaag tcggcgacaa
      841 agtttctgtc ggcgatgctt tttccgttgg cgaacgagtc gatgtgattg gtattagcaa
      901 gggtaaaggt tttgctggcg gtgtgaagcg ttaccacttc catggtatga acgccactca
      961 cggtacatct gaccgcaatc gtgcccctgg ttcacgtggc tccggtacga caccggggcg
     1021 tgtgtataag ggcgctcgtg gtgcaggtca tatgggcgtg gaccgcgtaa ctgcgcagaa
     1081 cattaaggtc gtgatggttg atccggaacg caatctgatt gccatcaatg gcgctgttcc
     1141 cggcggcaag gggagcctcg tcatgatcaa ggaatcgcgc aagcaatagc gcgcaggaaa
     1201 tcggagcgag aataccatga aagtagatgt tcttgatttg aagggcaaca aggttcgcga
     1261 agtggaactc cctgcgaacg tgtttgaagc ccctgtgaat gtagatctca tgcaccaggc
     1321 ttacactcgc cagatggcga atgcccgcct cggtacgcat gatacgaagg tccgtggaga
     1381 agttcgcggt ggtggtcgca aaccctggaa gcagaaggga accggccgtg cccgtcaggg
     1441 ttcacgccgt gcggctcagt gggtcggcgg tggtcgtatc cacaccccgc atcctcgcag
     1501 ctatgaactt cgcatgccca aaaagatgcg ccttgcggct ctccgctcgg ctttgtctgt
     1561 gaaggcttct gaagcttctc tcgttgttgt ggaagaactc aacttcgatg agcccaagac
     1621 taaggtcgtg gctcaggcgt tgggtaattt ggtcggatct tccaccgctt tggtgttgat
     1681 gccagccaag gaccagaatt acgatgtggt gatgcgcagt gcaagcaata ttgagagcgc
     1741 caaagtgttg ctcgcaagtt acttgaatgt gcgtgacatc cttaattttg agaaaatcgt
     1801 tttgcccgcc aaaacgatcg acgcgcttgt tgcgcaccta ggataggaga gagacatgag
     1861 cgatctttat aatgtcctcg tccgcccgct ggtgaccgaa aagtctaaca cccagtccag
     1921 caagtttggg cactatgtgt ttgttgtgaa taacaacgcc actcgcaccg ctgttaaaga
     1981 tgcggtcgaa acgctctttg acgtcactgt ggcgcgcgtg aatattgtca acgcccctgc
     2041 aaagcgcgga cgccgcggca aatctcgccg tatggttatt cgtcgtcctg ctttcaagaa
     2101 ggcgatcgtt acccttgctg agggcagtaa gcccctcgag atctttgaag gggtgcaata
     2161 atggcagtta aaaagtacaa accaattacc cccggcatgc gcgacatgac cggttacacc
     2221 tttgaggaaa tcaccaagtc cactccggaa cgctctttgc tcgttattaa gaagcgctct
     2281 ggtggtcgta atgcttacgg tcgtgtgacc gttcgtcatc gtggtggtgg cgctcgtcgc
     2341 tacatccgcg tggtggattt caaacgcgag aagttcggca tccctgcaaa ggttgcatcg
     2401 atcgaatacg atccgaaccg cactgcgcgc ctcgctcttc tgcactatgc ggatggcgaa
     2461 aaacgctaca ttgttgctcc cgtggatttg aaggttggtg acacccttat gtcgggtccc
     2521 aatgctgaaa ttcgctctgg caactctctt ccgatcagca atattccggt cggtacactc
     2581 attcacaata ttgagatgaa gcccggcaag ggcggacagc tcgtccgttc cgctggaggc
     2641 gcagctcaac ttctcgccaa ggaaggcgat tttgctcagg ttcgtatgcc ttctggcgaa
     2701 gtccgcctca tcttccaggt ttgttatgcc acgattggtc aggttggtaa tcttgatcac
     2761 agcaatgtca agcttggtaa ggctggtcgc aaacgccacc ttggtatccg cccaaccgtc
     2821 cgcggtactg caatgagtcc ccgcgaccat ccacacggtg gtggtgaagg ccgccagccg
     2881 gttggtcttc ccggtcctaa gtcaccttgg ggtaagccta cccttggtaa gaagacccgc
     2941 cttaataaga agacggatca gtacattgtg cgtcgtcgca gtaagacgaa ccgctagtag
     3001 atgaggtaag cagatatgtc acgatcattg aaaaaaggcc cgtacattga agcaaaattg
     3061 ctcaagcgta tcgaagccat gaatcagaaa aaagaaaaga aggtggttcg cacctggagt
     3121 cgcgcttctg tgatcttccc tcagatggtg ggtcacacaa tcgcggttca cgacggacgc
     3181 cgccatgtgc cgatttatat taccgaaaac atggttggtc accgtttggg cgaatttgcc
     3241 ccgacacgca cattccgcgg acatcaggcc gctgagaagg ctgcgtaagg agaataggac
     3301 atgactgata ttcaagctca cgttcgtttt ctccctcagt ccgcacagaa agttcgcgct
     3361 gttatcgacg tagtgcgcgg caagagcgcc attgaagcgt tggaaatcct caagtttgtg
     3421 aacaaacgtg ccgctggccc ggtttacaaa ctggttgctt ctgctgtggc gaatgctgaa
     3481 gaaaacttcg gtgtcagccg cgatgacctg ttcgttgcca agatcactgc agacgaagcc
     3541 ccgacccgca agtggcgtcg cttcggcgcc cgcggtcgtt tcaagccgct tttgcgccgc
     3601 agttctcatg taacagttat tttgcgcgag cgcggagcat aaggagatta tatgggtcga
     3661 aaagtacatc ccgttggttt tcgtctcgga atcaatcagc cgtggggcgg ccgttggttt
     3721 gctgagggaa gcacatatcg ccagcagttg caccaggatc ttgccatccg cggtttgttg
     3781 atgggcaagc tctccaaggg cggcgctgct gctaatgaaa aagttcgcga actgcgcaag
     3841 gaactccctg atgcaatccg cgatgccaag gctggtatct cccatgttga tgtggagcgc
     3901 acccctggca agatccgcat cgctattcac accgccaagc ccggtatttt gatcggtcgc
     3961 aaaggcgaag gtgtgaagaa gatccgcact gcgctcgaag cgttggttgg taagaagatc
     4021 gaattggaca tcaaagagat cacgaatcct gacctcgacg caagactggt tgccaagaat
     4081 attgctgacc agctcgaacg ccgtatcgcg tatcgccgcg ctatgaagcg tgccattcag
     4141 caagccatga agcagggcgc cgagggcatc aagatcctcg ttggcggtcg cttgggcggc
     4201 gctgaaatgt ctcgtgatgt ctggctccgc gaaggacgtg ttcctctgca gaccttgcgt
     4261 gctaaccttg atttcgccac cgacgaagct ttgaccactt acggtcagat cggcgtgaag
     4321 gtgtggattt ataagggtga agtggcaccg gaagttgaag aaaaaaccga agcgacggaa
     4381 ggcgtgtacg tcagcgagta atcgctgcgt cgcttgagat gaggttattg ctatgttgat
     4441 gcctaaacgt gtaaagtggc gcaagcagat gcgtggtcgc atgcgaggta aggctctccg
     4501 cggtgcggaa gtcgccttcg gcgaattcgg tctacaggcg ctcgaaccgg gttggattac
     4561 agcccgtcag attgaagcgg ctcgccgttc gatcgtccgc gaaatgaaac gccgcggtaa
     4621 gatctggatc cgtattttcc cggccaagcc gttcacacat aaaccacccg agactcgtat
     4681 gggttccggt aaagggaatg tggagtacta cgtcgctgtg gtgaagcctg ggcgcattat
     4741 gttcgaagta gcaggtctgt ctgcggacat tgccatcgct gctttgcgct ctgcccagta
     4801 caagttctcc atcaagacca aggtcgtggc tcgccacgcg ggaggagaat agtcatgaag
     4861 gctatgaaat ccgcagaaat tcgcaagctg ggtcttgagg aaatccgcac aaagatttcc
     4921 gatgcgcgcg atgagttgat gaaactgcgc ttccagcagg tgaccggtca gctgaccgat
     4981 accagccgct tgaacatcct ccgcaaggat attgctcgaa tggaaaccat tctggctgaa
     5041 atgctcagga atgctgcagt agaaggtgca aaatgaacaa tcgtcgtcgt atcactggtg
     5101 tagtcaccag caataaaatg accaaaacgg tcgtcgtgga aatcactcgc aagtatcgcc
     5161 atcctcttta taagaaggtt gtgttctcga gcatgcgcgt gaaagctcac gatgagatcg
     5221 gctgccagat cggtgatgaa gtccgcatcg tggaatcccg cccgttgtcg cgcgataagc
     5281 gttgggtggt tgaatccgtc gtcaagcgcg aagttcgtac tgccgatcag ggtgtaggag
     5341 aataagccat gatccagcat gaatctcgtt tgaaggttgc tgataatacc ggcgcccgtg
     5401 aacttctcgt catccatgtg atgggtggtt cccgacgcaa atatggtgct attggtgatg
     5461 tggtggtggc aactgtcaaa gctgctgctc cgcaaggctc tgtaaagaag agcgaagttg
     5521 tgaaagccgt gatcgtgcgc tgcaccaaag aatggcgccg cgaagacggc tcgtacatcc
     5581 gctttgacga taacgctgca gttatcctcg atgccgatgg cgaaaacccg aggggcacgc
     5641 gtatcttcgg acccgtggca cgcgaattgc gtgatatggg ctacatgaag atcgtgtcgc
     5701 ttgccccgga agtgctgtag gagctgagaa tgaaagttaa gattcgaaaa ggtgataccg
     5761 tcgaagtgat cagcggccgt ctcgaagata aaggcaagcg cggtgaagtc atcaatgtga
     5821 tccttgaaga acgccgtgtg gtggtgcagg gtgtgaacgt ccgcaccaag caccagaaac
     5881 aggtgcaagc tggcaaccgc cagatgaacc ctggtcgcgt tcagttcgaa ggtcctattg
     5941 atatttccaa cgtgatgttg gtctgcccga agtgcagcga agccacccgt gtgggtgtgc
     6001 aacgtgatga cgaaggcgca catcgtgttt gcaaaaattg tcaagccacg attgactagg
     6061 caccggagca ttagaacatg aacaaaatgc aagaacgtta taacaaagaa gtagccccgg
     6121 ctctgctgaa gtcattcggt ttcaagaatg tgatgcaggt tccgcggttg gaaaagatcg
     6181 ttgtcaacat cggtctcggt gaagcgttgg ataaccccaa ggctctcgaa gctgctgtga
     6241 ccgacctgac caccattacc gggcagaaac ccgtgcagac caaagctcgc aaaagcattg
     6301 cgaacttcaa actgcgtgaa ggtcgtttga ttggtaccaa ggtgaccctg cgtggtcccc
     6361 gcatgtgggc gttctttgac cgcctcgtga acattgcact tccgcgcgtg cgcgacttcc
     6421 gtgggatttc cccgaacgcg tttgacggac gtggaaacta tacgcttggt cttaaggatc
     6481 agttggtttt ccctgagatt gagtacgaca aaatcgataa attgcgcggt atggaaatca
     6541 caattgtgac gagtgcccag aatgatgatg aagcccgcgc tttgttaaga cagctcggta
     6601 tgccgttcag cggcaagaag gaagcgtaag gtatggcaaa gaa
//
LOCUS       DAOREF010000028         6533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6103_length_6533_cov_4.416950, whole genome shotgun sequence.
ACCESSION   DAOREF010000028 DAOREF010000000
VERSION     DAOREF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6103_length_6533_cov_4.416950"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKI33_01040"
     CDS             <1..1125
                     /locus_tag="PKI33_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNM35611.1"
                     /translation="MFATSNDLRPVEIGIFAPGMKPVDVIGSGEVGYVATGFKTVHEC
                     RVGDTLTLAASPAAEPLPGYFLPKPMVFAGIYPVEADDYSELREALEKLQLNDASLTY
                     QPETSQALGFGFRAGFLGLFHMEIIQERIEREYLLDVLFTAPSVEYEVLTIGDEVVTV
                     DSPAELPDPSKIVEVREPWMNIEIITPTDYYGAIMDLVTKRRGIFKQQEHPAPHRVQL
                     DFEIPLSEIIVDFFDHLKSRTKGYASLDYQFLEYRADKLQKLEILVNGDTVDALAAIV
                     HEKDAYHKGQRLITKLKDLIPRQLYDVAVQAASGGRIISRATVKATRKDVLAKCYGGD
                     ISRKKKLLEKQKRGKKRLKMVGNVEIPQEAFMAVLKLEDE"
     assembly_gap    1308..1407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1520..2548
                     /locus_tag="PKI33_01045"
     CDS             1520..2548
                     /locus_tag="PKI33_01045"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNM35612.1"
                     /translation="MKNKQYLVTGGAGFIGSNYVHRLLKRGEKVTIYDNFSRGGAPRN
                     LEWLKAEFGEKAFEVVVGDVRDAARIAEAAKTSDVIVHLAGQVAVTTSVTHPRDDFEA
                     NALGTFNVVEAARASGRQPIVIYASTNKVYGGMEEVELFEEPTRWRYKDLIHGCPETQ
                     PLDFHSPYGCSKGTGDQYVRDYARIYDLPTVVFRQSCIYGPRQFGIEDQGWVAWFIIA
                     AVMGRNITIYGDGKQVRDILHVHDLIQAYDLAVEKIDVARGQVYNLGGGPSNVMSIWS
                     EFGPRLEALLGKKIEVGRGDWRPGDQRVFYADVSKAKNELGWEPKISVEQGVNMLFEW
                     VQANKNLF"
     gene            2659..3489
                     /locus_tag="PKI33_01050"
     CDS             2659..3489
                     /locus_tag="PKI33_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNM35613.1"
                     /translation="MPHQLLSLVDVAMATLPNVLISLAIFAVSYYAGVWLGRLLKGVL
                     TRKNAEPGVSHLLSQILKWTIISLGFITALQRFFDVTAFLTGLGIIGFTIGFALQNIM
                     QNFVSGIILLIQQPFKVSDEVSLMGNEGFVQKIGLRTTEIRLRNGLLVFLPNADVLSQ
                     PIINFSRANGFRVELPVSIPYEADTEKARSIILDQVRTVPGCLETPVAQVFFHTFNAS
                     SVDLRVQFWVDNRTTDHHAAKDMVLVRIKQAFEENGIDVPYPVQVMKNPTKVTKRKTK
                     "
     gene            3486..4646
                     /locus_tag="PKI33_01055"
     CDS             3486..4646
                     /locus_tag="PKI33_01055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM35614.1"
                     /translation="MKILTVLTYYRPHTSGLTIYAERLAHAFVKRGHQVTVMTTQYDP
                     SLPREEMMDGVKVIRVPVLARVSKGVLAPTFGLVATKLAWEHDVVQMHLPQFDAPGVA
                     FRARLFGKPAVLTYHCDIQLPQGVFNRLVNAVVNFQNHMAGILSNHIVTYTQDYADHS
                     PYLSHYASKLTPILPPVELPDPLPGAVSAFASTHLTQERRPVIGMAARFASEKGVEVL
                     LDAMPAILKKYPKAQVLFAGTYQYVMGEQAYSDRLMPRIHEYEVNGNWKFLGNLDPVQ
                     MAAYYPNLDVITVPSLNSTEAFGLVQIEAMMNGVPSVPSALPGVRQPVKMHGMGVVSE
                     IGNSESLATAILEVLDHKEKYRGDLSAIKAAYNPDTVAQEYEKLFEKLMRKK"
     gene            4643..5413
                     /locus_tag="PKI33_01060"
     CDS             4643..5413
                     /locus_tag="PKI33_01060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM35615.1"
                     /translation="MNEQRSKDFVFLQVSSLPYFRALLRAVESEFYQDLPLPSPVYDV
                     GCGDGHFASLTFDHKLDVGLDPWRFSMKEAKRYNAYKSLVEADGAQTPFPSNHFASGL
                     SNSVLEHIPHIDAVLKETARVLKPGAPFYFCVPNANHFEELSLRKIFGKPYEMWFRRI
                     QRVQHSDHPDVWEERLERAGFKLERWWHYFSPSALRVLEWGHYFGLPSLVAKALTGNW
                     IISPTKWNLFLTEKYIRKYASTEPIENGTYTFYIARKK"
     gene            5590..>6533
                     /locus_tag="PKI33_01065"
     CDS             5590..>6533
                     /locus_tag="PKI33_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35616.1"
                     /translation="MESNEHTSPQEELTVLDLYKSVTKDWSSFFKFIRSLGDVRRREE
                     FNQALAQETAQAVMIEQTAEGSQPKRAAVDFPFRAVLALSIAFGAQFLIEPPNRFGML
                     SFALYLAAFGVAIWSYRSDEWRLPALPVAWQRFDPLSFKFIPLVIATVLAVLAFVDFG
                     SGLFSVRNTLIWFLAVAFICYSLWLQVAKTPETDSVDNAPNVRRKKWVQPVLFLIVFG
                     IALFYRLYSLNTVPNEPFSDHAEKILDVYDISQGYPKVFFERNTGREAIQMYWTLLVL
                     TVFNTGFSYLSLKLGTILLGILTLPYVYLLGKEYGSERL"
BASE COUNT         1632 a         1436 c         1515 g         1850 t
ORIGIN      
        1 atgttcgcaa ccagtaacga cctgcgccct gttgagatcg gaatttttgc acccggcatg
       61 aaacctgtgg atgtaattgg ctcgggtgaa gtaggctatg tagctaccgg cttcaaaacc
      121 gttcacgaat gccgcgtagg tgacacgttg acgctggctg cctctcccgc cgccgaaccc
      181 ctgcccggat acttcctccc caaaccgatg gtctttgcgg gtatttaccc cgtcgaagcg
      241 gatgattact ctgagttacg tgaagcgctt gaaaaacttc aactcaatga cgcttcattg
      301 acctatcaac ctgaaacctc gcaagcctta ggctttggtt tccgcgctgg tttccttggt
      361 ttgttccata tggagatcat tcaagagcgc attgaacgcg aatatttgct cgatgtgctt
      421 ttcacggcac cctctgtaga atatgaagtg ttgaccattg gcgatgaagt tgtcaccgtg
      481 gattcacctg ccgaattgcc agacccaagc aagatcgtcg aagtgcgcga gccgtggatg
      541 aatatcgaga tcatcacgcc gaccgattat tacggcgcca tcatggactt ggtcaccaaa
      601 cgtcgcggca ttttcaagca gcaagaacat cctgctccac atcgcgtgca gttggatttt
      661 gaaattccgc tctctgaaat cattgtggat ttcttcgacc acttgaaatc gcgcaccaag
      721 ggttatgcgt ctcttgatta tcaattcctt gagtaccgtg ccgataaact gcaaaaactg
      781 gagatcctcg tcaatggaga tacggtcgat gcattggcag ccattgtcca tgagaaggat
      841 gcgtaccata aaggtcaacg cctgattacc aagctcaagg atttaattcc gcgccagtta
      901 tatgatgtcg ccgtgcaagc cgcttctggc ggacgtatca tcagccgcgc tacggtcaaa
      961 gccacgcgta aagatgtgct cgcgaagtgt tatggtggtg atatctcacg taagaagaaa
     1021 ttgcttgaaa aacaaaagcg cggtaagaag cgcttaaaga tggtgggaaa tgtcgagatc
     1081 ccgcaagaag cctttatggc ggtcttgaag ttggaagacg aatagtgtat gcggattgca
     1141 cattatcata tgtaatgtgc aatccgcatt ttgtaaggaa cccccatgtc tgaatctcaa
     1201 aataatctaa aaagaatcct ccccggcgtt ttggttagca ttgcgctcat tgcagccatt
     1261 ctctactttg tggatattaa gaccaccttg aatgccttgc gcaatgcnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt tataactatc tcaatagtac tgttgtggga
     1441 atgctcgggt tatcgagcga agggcaaacc ctttcaggtg tttacaaaca actaattaat
     1501 tttaggaaca gaaaaacaga tgaagaataa acagtatctc gttacaggtg gtgccggttt
     1561 tatcggttca aattacgttc atcgcttgtt gaaacgcggt gaaaaggtga ccatttacga
     1621 taacttctcg cgaggtggcg ctccgcgtaa tttggaatgg ttgaaagctg agtttggtga
     1681 gaaggctttc gaggttgtgg ttggggatgt acgcgacgct gccagaatcg ctgaagcagc
     1741 taaaacgtca gatgtgatcg tgcatttggc aggtcaagtt gcagtgacca cttcggtcac
     1801 ccatcctaga gatgattttg aagccaatgc cttagggacc ttcaatgttg tagaagccgc
     1861 acgcgcgtcg ggacgtcagc ctattgtgat ctatgcttcc acgaataaag tgtatggcgg
     1921 catggaagaa gtagagctat ttgaagaacc tactcgttgg cgttataaag atttgatcca
     1981 tggttgtcct gaaactcagc ctttagattt tcactcgcct tatgggtgct ctaaaggcac
     2041 cggcgaccaa tacgtgcgcg attatgctcg tatctatgat ttgccaacgg tggttttccg
     2101 tcagtcttgt atttatggtc cgcgccaatt tggcattgaa gatcagggtt gggtggcttg
     2161 gtttattatt gccgcggtta tgggacggaa catcacgatt tatggcgatg gaaaacaagt
     2221 gcgcgatatt ttgcatgtac atgaccttat ccaagcgtat gaccttgccg ttgaaaagat
     2281 cgacgtggct cgtggacaag tttataacct tggcggcgga ccttcaaatg tgatgtcgat
     2341 ctggtcagag tttggtccgc gtttggaagc gttgcttggg aagaagatcg aagttgggcg
     2401 cggcgattgg cgccccggcg accaacgggt cttttatgcc gatgtttcca aagccaagaa
     2461 cgaacttggt tgggagccca agatcagcgt ggaacaaggg gtgaacatgc tgtttgaatg
     2521 ggtgcaagca aataagaatc tcttctaaag taaatatcaa acggctgtaa aagttcaacc
     2581 cgaattttta cagccgtact ttattaggta aaatacctag gctagatttt ccattcaaga
     2641 aaagaaaccc atccttatat gcctcaccaa cttttatctt tagtcgatgt tgccatggca
     2701 acactgccca atgttttaat ttcgctggca atttttgctg tgagttatta tgccggcgtt
     2761 tggctgggtc gtttactaaa gggcgtgctc acccggaaga atgccgagcc tggcgtttcg
     2821 catttgcttt cacagatcct taagtggacg attatttctc tcggcttcat cactgctttg
     2881 caaaggtttt ttgatgttac cgccttctta accggtctag gcatcattgg ctttaccatt
     2941 ggctttgcct tgcaaaacat catgcagaat tttgtctctg gcattattct cttgatccaa
     3001 cagccattta aagtaagtga cgaagtaagt ttgatgggga acgaaggctt tgttcagaag
     3061 attggtttgc gcaccactga gattcgctta cgcaatgggt tgctggtttt tctacccaat
     3121 gccgatgtgc tttctcaacc gatcatcaac tttagccggg caaatggctt tcgtgtggaa
     3181 ttgccggtaa gcattcccta tgaagccgat actgaaaagg cgcgttccat tattcttgac
     3241 caagttagaa ccgtgcccgg ttgtttggaa acacccgttg cccaagtatt ttttcatacc
     3301 tttaacgcct catcggtcga tttacgcgta cagttctggg tggataatcg cactaccgac
     3361 catcatgcag cgaaagatat ggtgttggtc agaatcaaac aagcatttga agaaaacggg
     3421 atcgacgttc cgtacccagt tcaagttatg aaaaatccaa ccaaagtgac gaaacgaaag
     3481 acaaaatgaa gatactgacc gtactaacct attatcgccc acatacctct gggctgacta
     3541 tttatgcaga acggcttgca catgcctttg tcaaacgcgg gcaccaagtc actgtaatga
     3601 ccactcaata cgacccatcc ctgcctcgtg aagaaatgat ggatggtgta aaagtgattc
     3661 gtgttcctgt gcttgcgcgt gtcagcaaag gagtgcttgc cccaactttt gggttggtcg
     3721 ctaccaaact ggcatgggaa catgatgttg ttcaaatgca ccttccgcag tttgatgcgc
     3781 ccggcgttgc cttccgtgcg cgtttgtttg gcaagcctgc tgtccttaca tatcattgcg
     3841 atattcaact tccgcagggc gtattcaatc gtttggtcaa tgctgtggtg aattttcaaa
     3901 atcatatggc ggggattctt tcgaatcata tcgtgacgta cacacaggac tatgcggatc
     3961 attctccgta cctctcacat tacgcctcaa aactgacccc catcctgcca cccgttgagt
     4021 tgcctgatcc actccctggg gctgtttcag ctttcgcttc cactcatctt acgcaggagc
     4081 gtagacccgt aattggtatg gcagcgcgtt ttgcttctga aaaaggcgta gaagtcttgc
     4141 ttgatgcgat gcctgccatc ctcaagaaat atcctaaggc tcaagtgtta tttgcaggga
     4201 catatcaata tgtgatgggt gagcaagctt attcagaccg cctgatgcca cgcattcatg
     4261 aatatgaagt aaatgggaat tggaaattcc ttggcaatct cgaccctgtt cagatggcag
     4321 catactaccc gaacttggat gttattaccg taccatctct caattcgact gaagcgtttg
     4381 gtttggtcca gatcgaagcc atgatgaacg gtgtgcccag tgtgccctct gctttgccag
     4441 gggtacgtca acctgtgaag atgcatggca tgggtgtcgt ctctgagatt ggcaattcag
     4501 aaagcctggc aactgccatt cttgaagtcc ttgatcacaa agaaaaatat cgcggtgacc
     4561 tttcagccat taaagccgca tataaccctg atacggtcgc acaggaatat gaaaaattgt
     4621 ttgaaaagtt aatgaggaaa aagtgaacga acaacgatcc aaagattttg tttttttgca
     4681 ggtgagttca ttaccctatt ttcgtgccct gttgcgggca gttgagtctg agttttatca
     4741 agacctgcca ttaccctctc cggtttatga tgtgggttgt ggtgatggtc acttcgcttc
     4801 actaacattt gaccacaagc tggatgtcgg gcttgatccg tggcgtttct ccatgaaaga
     4861 agcgaaacga tacaatgcct ataaatcctt ggtcgaagcc gatggcgcac aaacaccgtt
     4921 cccttccaat cattttgcca gcggattaag caactccgtt ctggagcaca ttccgcacat
     4981 tgatgcagtt ctaaaagaaa cagcacgtgt gcttaagcca ggagcccctt tttatttttg
     5041 tgtgccgaac gccaatcact tcgaagagct ttcgcttcga aaaatatttg gcaaaccata
     5101 tgaaatgtgg ttccgtagaa ttcaacgggt ccaacattca gaccacccgg atgtatggga
     5161 agaacgcctc gaacgtgcag gcttcaagtt ggagcgttgg tggcattatt tttcgccatc
     5221 tgctctacga gtcttagaat gggggcatta cttcggattg ccctctctag tggcaaaggc
     5281 attaacaggt aattggatca tttcacctac aaaatggaat ctctttttga ccgaaaaata
     5341 tattcgtaag tatgcatcca cagaaccgat tgagaacgga acatatacat tctatatcgc
     5401 taggaaaaaa taattaaaag gctgaaaata cggattttat tttcagcctt ttctgtctaa
     5461 aagcgccttg aaagggcgct tttcgctatt tttgccataa attcttaata tagtgcactt
     5521 aaacacgccc aaaattagta tagaattacc ctgttctgca acaatttaca ttttaggttg
     5581 agagagtata tggaatcaaa tgagcacaca tccccgcaag aagaactgac tgttttggac
     5641 ctttataaat cggtcacaaa ggattggtcc tcgtttttta agtttatccg ctcgttaggg
     5701 gatgtacggc gtagagaaga atttaaccaa gctcttgcgc aagaaactgc gcaagcagtg
     5761 atgattgagc aaacggcaga aggctcgcaa cccaaaaggg ctgctgttga cttcccattt
     5821 agagctgttc ttgctctaag cattgcattt ggtgctcaat tccttattga gcctcccaat
     5881 cgtttcggaa tgctatcctt tgcattgtat cttgctgcgt ttggggtcgc catttggtct
     5941 taccggtctg atgaatggcg tttgcctgct ttacctgttg catggcaacg atttgatcca
     6001 ttgtctttca agtttatccc tttggtgatc gcaacagttt tagccgtttt agcctttgtt
     6061 gattttggaa gcgggctttt ttctgtacgc aataccctta tatggttctt ggctgtagcg
     6121 ttcatttgtt atagtttatg gcttcaggtt gcaaaaacgc ctgaaacgga ctcagtagat
     6181 aatgcaccaa acgtcaggcg aaaaaagtgg gtgcagcctg ttttgttctt gattgttttc
     6241 ggaattgcac tgttttatcg tctatattca cttaatacgg ttccaaatga accatttagt
     6301 gatcacgctg aaaaaattct cgatgtatat gatatttcgc aaggatatcc aaaagtcttt
     6361 tttgaacgca atactgggcg tgaagcgatt caaatgtact ggacattgct tgtgcttact
     6421 gttttcaata caggtttttc ctacctcagt cttaagcttg gtaccattct ccttggtatt
     6481 ctgacgttgc catacgttta cctgctcggt aaggagtatg gaagtgaacg gct
//
LOCUS       DAOREF010000029         6484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6179_length_6484_cov_5.021776, whole genome shotgun sequence.
ACCESSION   DAOREF010000029 DAOREF010000000
VERSION     DAOREF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6179_length_6484_cov_5.021776"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKI33_01070"
     CDS             complement(<1..504)
                     /locus_tag="PKI33_01070"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNM35617.1"
                     /translation="MITSNQNSKIKLVRALLGRAKERREAGAFVAEGVRLVEEAVKAN
                     WTCRFALYDETLSERGKSQVESLRSRGVDVEEVSASVMKSISETEAPQGILAVLEQSQ
                     LPIPNSRNFILIPDQIRDPGNLGTLLRSAAASGVQAVLIPPETTDAFAPKVLRSGMGA
                     HFRLPIHA"
     gene            complement(501..1061)
                     /locus_tag="PKI33_01075"
     CDS             complement(501..1061)
                     /locus_tag="PKI33_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNM35618.1"
                     /translation="MNSILVEKKFSTGQSLQIVQGDITIEEVDAIVNAANEQLAHGGG
                     VAWAISKKGGPKIQAESDDWVRKHGQVSHAHPAWTSGGALPAKYVIHAVGPVWINGRD
                     DENQKLQAAVTGSLQVADELKCASISMPAISTGIFGFPKDRAAGIIFETIGDYFDKHE
                     GSTVNVVRIVLFDQSTIDAFLKQWAE"
     gene            complement(1124..1465)
                     /gene="rplT"
                     /locus_tag="PKI33_01080"
     CDS             complement(1124..1465)
                     /gene="rplT"
                     /locus_tag="PKI33_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNM35619.1"
                     /translation="MRVKGGPQGHLRHKKVLKITKGQRGSKSTLFKRANEAMLKSLWY
                     ATRDRRVRKRDLRKLWIARINAAARLNGTTYSKLVAAMKKANIELNRKMLADIAVRDP
                     QAFAAVVAQTK"
     gene            complement(1563..1796)
                     /locus_tag="PKI33_01085"
     CDS             complement(1563..1796)
                     /locus_tag="PKI33_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNM35620.1"
                     /translation="MPRKAKPGKKFKLKTHKATSKRFRLTGAGVLVRTKGGKSHLRRR
                     TSDRTKALLSETIVVKGRKFVKRIKRLAPNMEG"
     gene            complement(1887..2456)
                     /gene="infC"
                     /locus_tag="PKI33_01090"
     CDS             complement(1887..2456)
                     /gene="infC"
                     /locus_tag="PKI33_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNM35621.1"
                     /translation="MSANEFRVNEGIRVAEVRLIGPDGGNVGVVSIKEALKIAREAEL
                     DLVEVSPGANPPVCRVMDFGKFIYEKAKKEREAKKSQTKIEVKEIRLRPKTNEAHRGF
                     KVDDARRWLLQGHKVRVTVKFRGREMDYPEIALEDLKEIAADLADVAIIEVPPQMEGR
                     TMLVVMAPNKAAAPKKKEKPEQAEAKNEA"
     gene            complement(2561..5080)
                     /locus_tag="PKI33_01095"
     CDS             complement(2561..5080)
                     /locus_tag="PKI33_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM35622.1"
                     /translation="MSEQQLIQAGISALQSNDKQKAFSIFAQVVQQYPQSERGWFLLG
                     MSTDDTQRRKYCLQKVLSINPNHTDAKKQLTLLTPPQPAPPTPTFTSFNRSDFQQQHV
                     PAFVDNEPDATSEFERFSEPLNQPEVQQEPQHQPTPKQTKQIRRKKNNNVILIASISG
                     LCILAFTAMAGFYLLMNSRTTPAVQAANPTQPISATNTTLAMAPVATDTPIPPTPLPT
                     GLPTQPYTPRFEEAACQFDVPPGVNVSCGYAIVPENRTINSGRTIRLAVAVFHSRSSN
                     PKPDPVIFLQGGPGGEAVSLSANAYPILVDPFLSDRDYIAYDQRGTGLSEPALICDEL
                     ERANRQDVYGTIDSGSRELVYQNAFLSCAGLLQTQGVDLSAYNTVENAADLRDIVTLL
                     GYEKVNLYGASYGTRLALVAMRNHPEVIRSAILDSVMPVETNWVTEFPNIANYTLTNL
                     FNACKADPDCNKAYPDIETVFWELASKLDEKPITLSTSAYPMGTVTETIDGSYLLSII
                     MGLSKTTRFIDTAPQTIYRVRDGDYSTLIAAQYSLPYGFEGINTGLYISVLCREHVLD
                     TSPEKLLEAATQLRVKDFVWSPFYGHLDKMFSACKTWGSTGPVLGEKDKVVSDIPSLV
                     ITGTFDGATPPQQGKQVADSLPNSYYFEFPNQGHVPTASDDSGCAMQIALEFLDNPLT
                     EPSRDCMGSLKPVKFLVPYTGDPALELKRENLNGVSLIVPTEWKFVDDGFWWRGSASF
                     DITQVTAFRANISVQDLVDYFSSSINGYRGFDGAPTPAGTREANGHAWTLYYATSNGR
                     PVDVAAVDDKGSSMVIVMFSHPDEHDALYRTLFLPMVDSAK"
     gene            complement(5134..5457)
                     /gene="trxA"
                     /locus_tag="PKI33_01100"
     CDS             complement(5134..5457)
                     /gene="trxA"
                     /locus_tag="PKI33_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNM35623.1"
                     /translation="MSSVKYVTEQDFQDEVISSQTPVLVDFTADWCQPCKMIAPIVEQ
                     LAGQWDGKVKVVKLDADQNPNIMMQYGVMGIPTLMLFKGGDVKERITGYLPKDKIEAK
                     VTPHV"
     gene            5555..5626
                     /locus_tag="PKI33_01105"
     tRNA            5555..5626
                     /locus_tag="PKI33_01105"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5587..5589,aa:Lys,seq:ttt)
     gene            5699..6484
                     /locus_tag="PKI33_01110"
     CDS             5699..6484
                     /locus_tag="PKI33_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM35624.1"
                     /translation="MKLEVITRIPKEQKYKTPLLFVHGMWHGAWCWDEFFLPFFADAG
                     YHVTALSLRGHAGSEGKIQGSSIMDYVRDMEEVARTLPTPPVLIGHSMGGFIVQKFLE
                     RNTAPVGVLLAPAPPYGVWNGTWQVFKHSPLTLMKILTRFRLHPVVETPESTRWAFFS
                     ADMPQEQLLKYHTKMNDESFRMYLDLLGLNLVRTKKVKTPLLVLGAKEDTVISNHDVE
                     KTAQAYNTKAELFPSMAHDMMLEKGWKDVANRIMEWLKEKGIE"
BASE COUNT         1506 a         1587 c         1666 g         1725 t
ORIGIN      
        1 tgcatgaatg ggcagcctga aatgcgctcc cataccagat cgcagaactt ttggtgcaaa
       61 agcatccgtc gtttctggcg gaattaaaac ggcttgcacg cccgaagccg ctgcagaccg
      121 taacaacgtt cccaaattcc ccgggtcgcg gatctggtct gggatgagga tgaaatttcg
      181 ggaattggga attgggagtt gggattgttc gagcacagca aggataccct gcggcgcttc
      241 cgtctcgcta atggacttca tcacactggc tgagacttct tccacgtcca cgccgcgcga
      301 ccttaaactt tcgacctgag acttgcctct ttcgctcaag gtctcatcat acaaggcaaa
      361 gcgacatgtc caattcgctt tcactgcttc ttccacaagc cgcacccctt cagcgacaaa
      421 tgccccagcc tcacggcgct ctttggcacg ccccaacaag gcgcgcacaa gttttatttt
      481 tgaattctgg ttggatgtga tcattcagcc cattgtttta gaaaagcatc aattgtggat
      541 tggtcaaata gaacgatacg cacaacattt acagttgaac cttcgtgttt atcaaaataa
      601 tcccctattg tttcgaaaat aattccagct gctcggtctt tcggaaaccc gaaaatgccc
      661 gtagaaattg ccggcataga gattgacgca catttcaatt catctgccac ttgcaaagaa
      721 cctgtcacag cagcttgtaa tttttgattt tcgtcatctc tgccattgat ccacaccgga
      781 cccacagcgt ggatcacata cttcgcaggc agcgccccgc ccgaggtcca ggcaggatgc
      841 gcgtgtgaga cctgtccgtg tttgcggacc cagtcatcac tttctgcttg aatttttggt
      901 ccacccttct ttgaaattgc ccaagccact ccgccgccgt gggcgagctg ttcattcgcc
      961 gcgttgacaa tggcatccac ctcttcaatg gtgatgtcac cttgcacgat ctgtagagat
     1021 tgacctgttg aaaatttctt ttcgaccaga atggaattca tgaatcaatt ttacccccgt
     1081 taaaaattgt aggggcggat cgcgatccgc ccctacataa aaattacttg gtttgtgcta
     1141 cgactgcggc gaaggcttgt gggtccctca cggcgatgtc ggcgagcatc ttgcggttga
     1201 gctcaatgtt tgccttcttc atggctgcca ccaacttgct gtaggtggtg ccgttcaggc
     1261 gggccgcagc gttgatacgg gcgatccaca acttgcgaag atcgcgtttg cgaacgcggc
     1321 ggtcacgagt ggcgtaccag aggctcttga gcatggcctc attggctcgc ttgaacaggg
     1381 tgctcttcga gccgcgctgt cccttcgtga tcttcaaaac tttcttgtga cgtaagtgcc
     1441 cttgcgggcc tcctttaacg cgcattgttt agtacctcct gcaaactcgc gggcgtcggt
     1501 gggttacacc agttgcgcac acccgaaagc cgcacataaa aaatttacga cgaccggaag
     1561 atttagcctt ccatgttggg tgcgaggcgc ttgatgcgct tcacgaactt gcggcccttc
     1621 accacaatgg tctcacttaa gagagccttg gtgcggtctg aagtgcggcg gcgcaagtgg
     1681 cttttaccac ctttagtgcg caccagcacg ccagcaccgg tcaggcgaaa gcgtttggat
     1741 gtcgccttgt gggtcttgag tttgaatttc ttgccaggtt tagctttgcg tggcatcgcg
     1801 tagtactcct ttcagacaaa ataaaccgcg gacatttcac tgcccgcgga cgatcaacat
     1861 ctttgggtct tgctcttccc aacaggttac gcttcgttct tcgcctcagc ctgttcgggt
     1921 ttctctttct tttttggtgc tgcagccttg ttcggagcca tcaccaccaa catcgtacgc
     1981 ccttccattt gcggcgggac ttcgatgata gccacatcag ctaggtcagc ggcgatctct
     2041 ttcaagtctt cgagcgcgat ttcgggataa tccatttcgc gtccacggaa cttgacggtc
     2101 acacgcacct tgtgtccctg aaggagccag cggcgggcat cgtccacctt gaagccgcga
     2161 tgggcttcgt tggtcttcgg tcgcaagcgg atctctttga cctcgatctt ggtctgagat
     2221 ttctttgctt cgcgttcctt cttcgccttt tcgtagatga acttaccaaa gtccatcaca
     2281 cggcagacgg gtgggttcgc accgggcgat acctctacaa ggtccaattc cgcttcgcgt
     2341 gcgatcttca acgcttcctt gatggacaca actccgacgt tgccaccatc tggaccgatc
     2401 aggcgcactt ccgcgacgcg gatgccttca tttactcgaa attcgttggc gcttatgtgc
     2461 ttctcctctt gctgttagac aaaacaaacc cagcccatgg ctggtttttt agcgggcgaa
     2521 taataccatg aaggcaggtg agtcgtcaac gaaaaagggc ttattttgcc gaatctacca
     2581 tcggtaggaa gagtgttctg tataacgcat cgtgctcatc aggatggctg aacatcacaa
     2641 tgaccatcga acttcccttg tcatccactg ctgccacatc aaccgggcgc ccattggatg
     2701 tggcatagta caaggtccag gcatgaccgt ttgcctcacg cgtccctgct ggggtgggag
     2761 cgccgtcaaa accacggtag ccattgatgc tggaagagaa atagtccacc aagtcttgca
     2821 cactgatatt tgcacgaaat gctgtgacct gtgtgatatc gaaggaagcg gatccgcgcc
     2881 accagaagcc atcgtctaca aacttccatt cggtagggac aataagcgag actccgttga
     2941 ggttttctct tttcagttca agggcagggt cgccggtgta gggcaccaag aacttgaccg
     3001 gcttcagact tcccatacag tctcggctgg gctctgtaag cgggttatcc agaaattcca
     3061 aagcaatttg catggcacag ccgctgtcgt cgcttgcggt gggcacatgc ccttggttgg
     3121 ggaattcaaa ataatagcta ttgggtaatg agtctgccac ctgtttgcct tgttgtggcg
     3181 gagtagcacc atcgaaggtt ccggtgatga ccaaactggg aatatcgctg acgactttat
     3241 ctttttcgcc gagtactgga cccgttgagc cccaagtctt gcaggcagag aacatcttat
     3301 ccaaatgccc ataaaaaggg ctccaaacaa aatctttgac tcttaactga gtagcagctt
     3361 ccagcaattt ttcgggagat gtatccaaaa catgttcgcg acacagaact gagatataca
     3421 gacccgtatt aatgccttca aacccatacg gcaatgaata ttgagcggcg atcaaagttg
     3481 aataatcccc atcgcgtacg cgatagatgg tttgtggagc agtatctatg aaacgggtag
     3541 tttttgataa ccccatgatg atcgataata aataggagcc atcgatggtc tccgtaaccg
     3601 ttcccatcgg gtacgcagat gtagataaag ttatcggttt ttcgtctagt ttactggcaa
     3661 gctcccaaaa tacggtttca atatctgggt aggctttatt gcaatcaggg tctgctttgc
     3721 aggcattaaa taaattcgtt agagtgtaat tggctatgtt gggaaattct gtcacccaat
     3781 tggtttctac cggcatgact gaatctaaaa tagcgctgcg aataacctcg ggatgattgc
     3841 gcatggcaac caatgcgaga cgtgtcccat atgaagcgcc atataaattg accttttcgt
     3901 atcccagtaa tgtgacaata tctcttaagt cggctgcatt ttcaaccgtg ttatacgcgc
     3961 tgaggtcaac gccttgagtt tgtaaaagcc cggcacaaga gaggaaggcg ttctgataca
     4021 ccaactctct ggaaccgcta tcgatagtac cgtaaacatc ttgtcgattg gctcgttcaa
     4081 gctcatcgca gatcaaggct ggttccgaaa gcccggtgcc gcgttggtca taagcgatgt
     4141 aatctcggtc actcaaaaat ggatccacta agattgggta ggcatttgcg ctgagcgaaa
     4201 ccgcttcgcc gcccggacca ccctgtaaaa agataaccgg gtctggtttg gggttggaac
     4261 ttcggctatg aaatacagca accgccaagc ggatggtccg cccgctgttg atagtgcggt
     4321 tttcaggcac aatggcataa ccacaagaaa cgttcacccc gggcggcaca tcgaattgac
     4381 aagctgcttc ttcaaagcga ggtgtgtatg gctgtgttgg taagccggtg ggaagtggtg
     4441 taggtgggat gggcgtatcc gtcgcaacag gagccattgc caaggttgtg ttcgtggctg
     4501 aaatgggctg tgtcgggttt gctgcttgaa cagcaggtgt cgtacggctg ttcatcaaca
     4561 aataaaagcc agccatggcg gtaaaggcca gaatgcaaag tccgctaatg gatgcgatca
     4621 ggatgacgtt gttattcttc ttgcgacgta tttgcttggt ttgttttgga gtaggttggt
     4681 gctgcggctc ttgctggacc tcgggctgat tcagcggttc tgagaatcgc tcaaattccg
     4741 aagtggcatc tggctcattg tcaacaaatg cggggacgtg ttgctgttgg aagtctgagc
     4801 ggttgaagga cgtgaatgtt ggggttggtg gagcaggctg gggcggggtg agaagcgtga
     4861 gctgtttttt ggcatccgtg tgatttgggt tgatactcag aactttttgc aggcaatact
     4921 tccgtctttg agtatcatct gtgctcatgc caagcagaaa ccagccacgc tcagattgag
     4981 ggtactgttg tacaacttgg gcaaagatcg aaaatgcctt ttgtttatca ttgctttgta
     5041 gcgcagaaat tcccgcctga ataagttgtt gttctgacat attttcccta tataaaagaa
     5101 aatggagccg actgtgttgg ctccattata ctcttacacg tggggggtga ctttggcttc
     5161 gatcttgtct ttgggtagat aacctgtaat gcgttctttg acgtctccgc ctttgaaaag
     5221 catcaaggtg gggatgccca tcacgccgta ctgcatcatg atgttggggt tttgatccgc
     5281 atcaagtttg acgaccttga ccttgccatc ccattgaccg gcgagctgtt cgacaatggg
     5341 tgcgatcatc ttacacggct gacaccaatc tgcggtgaaa tctaccaata caggggtttg
     5401 cgagctaatg acttcgtctt gaaagtcttg ttctgttaca tatttgactg aactcatggt
     5461 tgcctccgag gtcttggatg agatgttctc tattttaatt acttccgttg acgtgagtat
     5521 gctgccgtga tagaatgcaa tccgctttaa agatgggcgc gtagctcaat ggcagagcag
     5581 cggcctttta agccgtttgt tgagggttcg atcccctccg cgctcacaga aaatccctta
     5641 cctatgtgag ggatttttgt tttatacagt aaactcaaaa gggagaggag agtttgccat
     5701 gaaacttgaa gtgatcactc gaattcctaa agaacaaaaa tataagaccc cgctgctttt
     5761 cgtccatggc atgtggcatg gagcctggtg ttgggacgag ttctttttgc ccttttttgc
     5821 cgatgcgggg tatcacgtca cggcgctcag cctgcgtgga catgccggta gtgaggggaa
     5881 aattcaaggc agtagtatca tggattatgt tcgagatatg gaagaggttg cgcggacgct
     5941 tccaactccg cccgtgctga tcggtcactc catgggcggg ttcatcgtcc agaaatttct
     6001 tgaacgaaat acagccccgg tgggtgtttt gctggctccg gctccgccgt acggagtttg
     6061 gaatgggacg tggcaggtat tcaaacatag tccgcttacg ttgatgaaaa tcctcacccg
     6121 atttcgttta catcctgtcg ttgagacgcc tgaaagcaca cgttgggcat ttttctcggc
     6181 agatatgcca caagaacagc ttttgaagta tcacaccaaa atgaacgacg agtcgtttcg
     6241 tatgtattta gacttgctgg ggttgaactt ggtccgcacg aaaaaagtga aaacaccgtt
     6301 gcttgtcctt ggtgcaaaag aggacacggt aatttctaat catgatgtag aaaagaccgc
     6361 ccaagcctac aataccaaag cggaactatt tcccagcatg gcgcacgata tgatgcttga
     6421 aaaaggctgg aaggatgtag cgaataggat tatggaatgg ttgaaggaaa aaggaatcga
     6481 gtaa
//
LOCUS       DAOREF010000030         6475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6197_length_6475_cov_4.264798, whole genome shotgun sequence.
ACCESSION   DAOREF010000030 DAOREF010000000
VERSION     DAOREF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6197_length_6475_cov_4.264798"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKI33_01115"
     CDS             <1..345
                     /locus_tag="PKI33_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35625.1"
                     /translation="RMNAVIRDGVDAFLLVGSRASCERAAKTAAQHALLKLGDAKPRL
                     ALVLVDVAWQMLLKAQPGAEIAAVQEILGDNVPVAGGYTLGQVVTGDNETKPKFLNQH
                     IIVIVFGEPKEQ"
     gene            489..3119
                     /locus_tag="PKI33_01120"
     CDS             489..3119
                     /locus_tag="PKI33_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNM35626.1"
                     /translation="MTDKKAPTKKPTKKQTPEQDIQAGDSSVAVGGSVTDSAIIHGQT
                     IVLADRFWRDLKPALPPDAIKEATAAFLAYLTDRHYYLSLKGMGVSDRVPLRLKLLDL
                     YVPLKARMELPEGETWKRDLSLAGRDIAEYEEPLHFGDPVPLLNVLKSYSGVVVLGDP
                     GAGKTTFVKYLAVRLARGEGAKMGLDDYLPILLPLAAFANALQSDDIRLDDFIADYFS
                     GIGADLPIGPMLKEALKAGRALILLDGLDEVRDINMRNTVVERVVDFYTFHRHQGNKF
                     VLTSRVVGYRAVRPSAEDLAECTIVDFEDDEIEDFVERWTTALEKQAQGHTQVAQADA
                     EADRRDLLDAIQHNAGVRQLASTPLLLTILALMKRQGVTLPERRVQLYDQYVSTLLST
                     WNRARSLSGRAPGRDIDEIQTVRILAPLALWMHEVSPGVGLVGREDMRRKLEELFHER
                     GDLSPIQAARQFLMDVREHAALLLERGPGEYGFIHLTFEEYLAAVALALMGQGNSQPI
                     IETLSRHVGEQAWREVTLLTIGYLGIRQQLPKIAGEVVDALVDVQPGPPGEAVVLAGD
                     AVLDTYPNGVPLKSRDHVIEALVNTMQDGNIRPELRRHSGILLGRLGWRPGDLDRFVE
                     VPAGEFQFGVRKEPKNIPQMYFIAKYPVTNIQYARFVKEDGYQNREFWSDLGWEWRTG
                     KFDQRTMEAIERDWLEHRPLAKRSTPYYWHNIDVSNPIVPVVGVCWFEAEAYCKWLSK
                     KIVAVPEGYTIRLLTSEEWERAARGTDGREYPWGDGFIKSAANTWDSDATGSGLGGST
                     AVVTFAQGSSPLDVWDMSGNVWEWTGSWYDEDRKYRIVRGGSWIGYQWFARSSFCNWS
                     IPLMFNDDLGFRVAIAPKIA"
     gene            3128..3523
                     /locus_tag="PKI33_01125"
     CDS             3128..3523
                     /locus_tag="PKI33_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35627.1"
                     /translation="MAQKSKQGSHVSAGDGSIVIGGDVSGSNVSLFNNNVKLTPLFKT
                     IEKAVEKNNNLAPSDKADVTAELQEIRNELESTAPDETFLARRFRNIKRMAPEIVEVA
                     FETLKNPVGGVAEVVKRVAAKMAEDANAK"
     gene            3573..4253
                     /locus_tag="PKI33_01130"
     CDS             3573..4253
                     /locus_tag="PKI33_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35628.1"
                     /translation="MKRTFFSLLLVLTLTACFAPVDALPAPTAIASDTPQMAIPSPTI
                     LPATAIPAVDTPTPQIATSGFCQDARNLQLIKDLRTAIQNRDGELLAALVNPTTGVGV
                     RFIRNGNVITYFDNIKFIFETTYAADWGLAAGSGEPVKGSFQEIVLPSLDRAFTANAV
                     ITCNELQVGGATYTPEWPYADMDFYSVHTPGTDQYAGMDWETWAVGLVQQDGNPMLAA
                     LIHYAWEP"
     gene            4396..4815
                     /locus_tag="PKI33_01135"
     CDS             4396..4815
                     /locus_tag="PKI33_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNM35629.1"
                     /translation="MSCADEYTPQLRARGYRMTSQRLAILHVLHHTGKHLSPVEVYDQ
                     ARTELPSLTETTVYRTLEFLAENGLVRPALTSSGHLAYEIARHEHHHLVCRTCGSEME
                     VEHELLKSMYETLETESGYKLTDSHLTFFGLCPECQK"
     gene            4830..5768
                     /locus_tag="PKI33_01140"
     CDS             4830..5768
                     /locus_tag="PKI33_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNM35630.1"
                     /translation="MLYSPVPMHIPDGFLSLVVSIICWVVTALIVGAAISRTNRSLGE
                     KQIPLMGVMAAFIFAAQMINFPVAGGTSGHLLGGALAAIVLGPWAGMLVMTAVIAVQA
                     LLFQDGGLLVMGANILNMGLLTAAIGYGLYRSVAGQSKSVKLAVAGVAAWLSVMAGAL
                     LTSIELWLSGTSNLQIVIPAMLGVHALIGLGEALITVSALAFIMQTRPDLLGAGSESA
                     KGSPTWAYVGGAIALFVVLLSPFASADPDGLERVATDMGFINAGQSAPYQIIPDYTIP
                     FLGETAISTIVAGVVGVIVVAAIIILVGRGMKAKSS"
     gene            5827..>6475
                     /gene="cbiQ"
                     /locus_tag="PKI33_01145"
     CDS             5827..>6475
                     /gene="cbiQ"
                     /locus_tag="PKI33_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNM35631.1"
                     /translation="MHFDAFDRYHEKESFLHRLDPRVKVLVTVTFIISNALLPDAAWV
                     AFAFAWLFLLACNLLSKLGITYTLKRSIVAFPFALIAITVLFSMPGKPLATFHLMMSE
                     YTITDMGLLRFLSILVRSWLSVQMAILLVATTRFPDLIHAFEHLRVPAVLTTIIAFLY
                     RYLFVLTDEVFRLLRAREARSAAVAGSRSGRGVMWRAKIAGNMAGQLFLRSYERSD"
BASE COUNT         1536 a         1503 c         1692 g         1744 t
ORIGIN      
        1 cgcatgaacg cggtgatccg tgatggtgtg gatgctttct tgttggtggg gagccgcgcc
       61 tcttgcgaac gtgctgccaa aacagccgct caacacgccc ttctcaagtt gggggatgca
      121 aaaccccgcc tcgcgcttgt gctagtagat gttgcctggc aaatgctgct caaagcccag
      181 cccggtgccg agatcgccgc tgtgcaagaa atccttggcg ataatgtacc cgtggcaggc
      241 ggctatactc ttgggcaagt ggtgactggc gataacgaaa ccaagcctaa atttctcaac
      301 caacatatca ttgtcatcgt ttttggcgag ccaaaagaac aataaaataa agtacaaaag
      361 ccgcccgaaa ggcggctttt tcgtatagtc acgtaagggc tttcccagaa acacccttcc
      421 ttgcaagatg gtaaagccac ccatacccag attattgtat actgaatcta cacacatcga
      481 ggccgggtat gactgacaaa aaagccccaa ccaagaaacc aactaaaaaa caaacaccgg
      541 aacaagacat tcaggcggga gattcaagcg ttgccgtagg cggttccgtc acggatagcg
      601 ccattatcca tggacagacg attgtgcttg cagaccgctt ctggcgggac ctcaagcctg
      661 ctctgcctcc cgatgcgatc aaagaagcca ctgcggcttt tctcgcctat ctcacagacc
      721 gacattacta tttgagcctg aaaggtatgg gggtctcaga ccgtgttcca ctgaggctaa
      781 aactgctgga cctctatgtt ccgctaaaag cgcgtatgga actcccagaa ggggagactt
      841 ggaagcgtga cctaagccta gctgggcgcg atatagcgga atatgaagag cctttgcatt
      901 ttggtgaccc cgtcccattg ctgaatgttc ttaagagtta ttctggggtc gttgtattag
      961 gtgaccctgg cgctggtaag acgaccttcg tcaagtacct tgctgtccgt ttggcgcgtg
     1021 gggaaggcgc aaagatgggg ctagatgact atctcccgat cttgctccca ttggcagcat
     1081 ttgcaaatgc actacagagt gatgacatcc gcttggatga tttcattgcc gattatttct
     1141 ctggcattgg tgccgatctt cccattggac ccatgctcaa agaagcactc aaagctgggc
     1201 gtgcgctcat tttgttagac ggactcgatg aagtccgtga tatcaacatg cgcaacacag
     1261 tggttgaacg ggtagtagac ttttatacgt tccaccgtca tcagggcaat aaatttgtgc
     1321 tcaccagccg cgtagtgggg tatcgcgcag tacgcccctc ggctgaagat ttagccgaat
     1381 gcaccatcgt tgactttgaa gatgacgaga tcgaagattt cgtagaacga tggacaactg
     1441 ctcttgagaa acaagcacaa gggcataccc aagtggctca agctgacgcc gaagcagacc
     1501 gccgtgacct cttagatgct attcaacata atgcaggtgt acgtcaactt gcttccactc
     1561 cattgttgct caccattctg gcgttaatga aacgtcaggg ggtgaccctg ccagaacggc
     1621 gcgtacagct ttacgatcaa tacgtctcca ctcttctctc cacttggaat cgtgcccgca
     1681 gcttaagcgg acgcgctccc ggacgtgaca tcgatgagat tcaaaccgtg cgtattctcg
     1741 cgccgttagc cttgtggatg catgaggtga gcccgggcgt agggctggtg gggcgtgaag
     1801 acatgcgccg aaaattggaa gaactcttcc atgaacgcgg cgatctttct cccattcaag
     1861 cggcacgtca atttttgatg gatgttcgtg aacatgccgc tctactgctt gaacgcggac
     1921 cgggcgaata tggtttcatc catttaacct ttgaagaata tcttgcggca gtggctttgg
     1981 cattgatggg gcaaggcaat tcccagccga ttattgaaac cttatctcgc catgtgggag
     2041 aacaagcctg gcgtgaagtg accttgctta ctattgggta tcttggcatc cgtcagcaat
     2101 taccaaagat cgccggcgaa gtcgtcgatg cattggtaga cgttcaaccg ggtccgcccg
     2161 gagaagcggt ggtgttggct ggcgatgccg tattggatac ttatcctaat ggcgtaccgc
     2221 ttaagagccg ggaccatgtc attgaagcct tggtcaacac catgcaagat ggcaatatcc
     2281 gcccagagtt gcggcggcat tctgggatat tgcttggtcg ccttggttgg cgccccggag
     2341 acttggaccg gtttgtggaa gtgcccgctg gtgagttcca gtttggggtc cggaaagagc
     2401 cgaaaaatat tccgcaaatg tatttcattg cgaagtatcc ggttacgaac atccaatatg
     2461 cgcgttttgt caaagaagat ggatatcaga accgcgaatt ttggagcgac ctgggttggg
     2521 aatggcgcac agggaagttc gatcagcgca cgatggaagc gattgaacgt gattggctgg
     2581 agcatcgtcc gctggcaaaa cgcagcacac catattattg gcataacatt gatgtgagca
     2641 acccaattgt gccggtagtg ggggtgtgtt ggtttgaagc tgaagcgtat tgcaaatggc
     2701 tttcgaaaaa gattgtggct gtgccagaag gctataccat acgtttgctc accagcgaag
     2761 aatgggaacg tgcggctcgc ggtacagatg gacgtgaata cccatggggc gatgggttta
     2821 tcaagtctgc tgccaacaca tgggatagcg atgcaaccgg ttcggggctg ggtggtagta
     2881 ctgctgtggt tacctttgct caaggtagta gtccattgga tgtttgggat atgagcggta
     2941 acgtttggga gtggacaggt tcttggtacg acgaagatag aaaatatcgc attgtgcgtg
     3001 gcggttcatg gattggttat cagtggttcg cgcgttcttc tttttgtaac tggtcgattc
     3061 ctttgatgtt caatgatgat ctaggttttc gtgtggcaat tgcacccaag attgcatagg
     3121 agtaaatatg gcacaaaaaa gtaaacaagg aagtcatgta agcgctggcg atggcagtat
     3181 tgtgatcggt ggagatgtaa gcggtagcaa cgtttcgctt ttcaataaca atgtgaaact
     3241 cacacctttg tttaagacca tcgaaaaagc ggttgaaaaa aataataatc tcgccccttc
     3301 agataaagcc gatgtgactg ccgaattaca agagattcgc aacgagctgg aaagcactgc
     3361 ccccgatgaa acttttcttg cccggcgctt ccgaaacatc aaacgcatgg cacccgaaat
     3421 tgttgaagtt gcctttgaaa cattgaaaaa tcctgtgggt ggtgtggcag aagtggtcaa
     3481 gcgggttgcg gcaaagatgg ctgaagacgc aaacgcaaaa tagtacaatg aactggcgga
     3541 gaccccgcca gttttctttt gaaaggatac ccatgaaacg gacgtttttc tctctgttac
     3601 ttgttctgac ccttacggct tgcttcgccc ctgtggatgc acttccggct ccaactgcca
     3661 ttgcttctga tacaccccaa atggcaattc cctccccgac tattttgcct gccaccgcaa
     3721 tccctgcagt ggatactcct acaccacaaa ttgctacatc aggtttttgc caagatgcgc
     3781 gaaacctgca actcattaaa gatttacgta ccgccattca aaaccgtgac ggtgaattac
     3841 tcgctgcctt ggttaaccca actacgggtg taggtgtgcg ttttattcgt aatggaaatg
     3901 tcatcaccta ctttgataac atcaaattta tctttgaaac tacttatgcc gcagattggg
     3961 ggcttgctgc tggtagtggg gaaccggtga aaggctcttt tcaagagatt gtcttgcctt
     4021 ccctagaccg agctttcact gcaaatgcgg tgattacctg taacgaactc caagtaggtg
     4081 gtgcaaccta tactccagaa tggccttatg cagatatgga cttttactcc gtccacacgc
     4141 cgggcacaga tcaatatgcc ggcatggatt gggagacttg ggcagtaggt cttgtgcaac
     4201 aagatggcaa ccccatgctt gctgccctca tccactatgc gtgggagccg tagtaatcta
     4261 aagacagact aagaaaacat ggacggggca atatgtgggg gtattatccc gtccatgtgc
     4321 tgtttaatgt aagaaaaccc cggctgtgat attctaataa ctatactatt gcaagaagtt
     4381 gcaataagga gagctatgtc ttgtgctgat gagtacaccc cacaattacg cgcccgcggg
     4441 taccgcatga cctctcaacg gttggcgatt ctgcacgtgc ttcatcacac aggcaaacac
     4501 ctctcgccgg ttgaggttta cgatcaggct cgcactgaac ttcccagcct taccgaaacc
     4561 acagtctatc ggacgttgga atttctggct gagaatggct tggtacgccc ggcattgacc
     4621 agttctgggc accttgccta tgagatcgcc cgacatgagc accatcattt ggtttgccgc
     4681 acctgtggaa gcgaaatgga ggtggaacac gaattactta aatccatgta tgaaaccttg
     4741 gaaaccgaaa gcggctataa gttgaccgat agccacctta ctttttttgg tctttgtcca
     4801 gagtgtcaaa aataaaagga gagattcaca tgttgtattc cccagttcca atgcatatcc
     4861 ccgatggttt cttgagcctg gttgtttcga tcatctgttg ggtcgtaact gccttaatcg
     4921 tgggtgctgc gatttcccgc acaaatcgtt ccttaggtga aaaacagatc ccgttaatgg
     4981 gtgtgatggc agcttttatc ttcgctgcgc agatgattaa cttcccggtt gcaggtggga
     5041 catctggtca tcttttaggc ggtgctttgg cagcgattgt tttaggtcca tgggctggca
     5101 tgttagtgat gacggctgtt attgcggtgc aagcgttgct atttcaagat ggcggtctgc
     5161 tcgtaatggg tgcgaatatc ctcaatatgg ggctgttaac ggctgccatt ggttatggtc
     5221 tttaccgttc tgtggcaggg caatcaaaat cggtcaagtt ggcagttgct ggggttgccg
     5281 cctggctttc tgtcatggcg ggagctttat taacctccat tgaactttgg cttagtggaa
     5341 cttcgaattt gcagattgtt atccctgcca tgttgggtgt gcatgcccta attgggttgg
     5401 gtgaggcgtt gattacggtt tcagcgttgg cattcattat gcaaacccga ccagatttgc
     5461 ttggagcagg ttctgagtct gcaaaaggaa gcccaacttg ggcttatgtg ggtggtgcca
     5521 ttgctttatt tgtcgtatta ctttctccct ttgcttcagc agacccagat ggcttggagc
     5581 gcgttgctac agatatgggc tttatcaatg cagggcaatc agcgccatat cagattattc
     5641 cagattacac cattccattc ctaggtgaaa ctgccatttc tacaattgta gctggtgtag
     5701 tgggtgtgat tgtagttgcc gctattatta tcttagttgg tcgtggaatg aaggcgaaat
     5761 cttcataagt tgatatagaa tcgaggtgcg taaattaata atttacgcac ctcggaatag
     5821 gtttatatgc attttgatgc ttttgatcgt tatcatgaaa aggaaagttt tcttcaccgg
     5881 ctcgacccgc gtgtgaaagt tttggtaacg gttactttta ttatttcgaa cgcactgttg
     5941 ccagatgccg catgggttgc ttttgccttt gcctggctct ttttactggc ttgtaacctg
     6001 ctttcaaaac ttggaattac atacaccctc aagcgatcta ttgtcgcttt tccctttgca
     6061 ttgatcgcta ttacggtact gttttcaatg ccaggaaaac cgctcgctac atttcacttg
     6121 atgatgtctg agtacaccat cactgatatg ggtctgcttc ggtttttgag tatcttggtt
     6181 cgttcttggc tatcggtgca gatggcgatc cttttggtgg ctaccacgcg ctttccagat
     6241 ttgatccatg cttttgaaca tttacgcgtc ccagccgtgt tgacaacgat cattgccttt
     6301 ctttatcgtt atctgtttgt gctaacggac gaggttttcc gtctgctgcg ggcgagggaa
     6361 gcgcgttcgg cagcagtagc aggctcaagg tctggcagag gcgtgatgtg gcgtgcgaaa
     6421 attgccggga atatggcggg tcaattattc ttgcgtagct acgaacgcag cgacc
//
LOCUS       DAOREF010000031         6421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6297_length_6421_cov_3.094094, whole genome shotgun sequence.
ACCESSION   DAOREF010000031 DAOREF010000000
VERSION     DAOREF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6297_length_6421_cov_3.094094"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..484
                     /locus_tag="PKI33_01150"
     CDS             41..484
                     /locus_tag="PKI33_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35632.1"
                     /translation="MEIDLEFGDENNWVGITAIVLLVMIALVGLASIGERMTPVDELG
                     QPRVMNADDWRVHEATRAYNEEISVLRDDIREVSLLLQKSPNPIAAQILYERVAGHAN
                     NGQSTTQIARQLTVTAAESVLAWSNGLVDRASAMNAVNKAIEVLK"
     gene            490..1743
                     /locus_tag="PKI33_01155"
     CDS             490..1743
                     /locus_tag="PKI33_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35633.1"
                     /translation="MPDIAFHGQRSLSDLLEAERSMRAASDETVRGLRDVLLSVANRL
                     DGKRMEALRHVDPKIPGNWSAKQWKEFVDAIPAPDAWGAMTTAAISMSNNEENSRYVE
                     ELKAEIQNLMVDLENANTRIADMEKQQEAILLKTVRKNPVELKPMPSTRPNGYVFPPL
                     DALVHEAESRLLLFPNPPGKLMNGLVSVGSRDGKNRLIFLQRVFVSLFALGARGISNT
                     LELNHLVALPGEAQPNSGSVGKVLNDIEKNGLIAIERFEMSEPKSTLKVAKLTEKGEA
                     LFKGLFPKEKLIESEVSASMQWAHNDRFRATALMYFAALARKRGYYTSMNSNQESDLW
                     LGRGEESKYTILVDSLTDNRSQALAKLNNGKTCICATNKSIMERVAGNCGVSGIGGEA
                     TDLETILTHKYVDITPNDPLWRMTW"
     gene            1940..2770
                     /locus_tag="PKI33_01160"
     CDS             1940..2770
                     /locus_tag="PKI33_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35634.1"
                     /translation="MLANKLMTLAGIPWSTVEVELDKELPADAYSPVPGGASLTDIDP
                     AFMRDEFNRLFGVCGFGWGYEYDEDNVVLDTVKRGQGTAFSAIIKKLIFWVKLLDDEG
                     KEVILKIASSGGSVNDVQGYALSGAITNAIGKAASNIGFQKSVYLGARSHKNVGAKSP
                     KPAATSAAPPKATQAVKKPAAPAKPAAQAPKPQPKIQEPIDDEIIDDEPAAAVSEASN
                     FVISFGNRKGQKLGDVDLKYIAWYANDMKTEGDPEKVALQNAAKELLKIRSNGHAVPA
                     "
     gene            3009..3887
                     /locus_tag="PKI33_01165"
     CDS             3009..3887
                     /locus_tag="PKI33_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35635.1"
                     /translation="MATAKRKASQDRRRSFGERFDLNTVSLTGPVIAVWGRNGDVFVR
                     IQSPNLLSLPGPQSYVNIVIPGGMIDGDPVTLQKGDLLRVTGYVRQSNFQESLRLILE
                     GAGKRTFFDRSEILQEDRLAWKRIYIKRRNSIVQALKVEVLVAPAESQNFVDLEGIVS
                     EAWLYPRDDHEDLFFRIAIYDEHTIETANEGNFGRKKRYAHYINILLPEQRPVSSPDE
                     RRISIDIKQRVRVRGELRCTWSVESVHDLLFSTGRSDIAGLLQRSGAVSDELKTINTQ
                     LETLTVVANALVLYSR"
     gene            4530..5177
                     /locus_tag="PKI33_01170"
     CDS             4530..5177
                     /locus_tag="PKI33_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35636.1"
                     /translation="MYYRKLFVLITVLSLALSACSVIKAEPTMSLEDIQATAQSVALT
                     SVAQTEMAKPTEPPTQTPIPPTPIVEVTEEPVTEVVQIPQEVAPIVPPPTEPAPVVIV
                     QPTDASSTGDGQCDPYLTAGDKGARTSVTIKNQSAGPMTLSIYLYPTKLNACGSTSVT
                     LESKSETTMELIEGCYYLHAWINGKKPSNASAEYCFKVADGPRKWIVTAEVIEQR"
     gene            5376..5567
                     /locus_tag="PKI33_01175"
     CDS             5376..5567
                     /locus_tag="PKI33_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35637.1"
                     /translation="MTAFFNMFQGNKQKDAETLEKILPKLSPASQEEVKRFLDGSLAE
                     LSNFAKTEASIASLKGGVA"
     gene            6056..>6421
                     /locus_tag="PKI33_01180"
     CDS             6056..>6421
                     /locus_tag="PKI33_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35638.1"
                     /translation="MKTLFRKRMTQILSLVLSVYCATVLICTDNKSLHLFCDGDMFIL
                     IDHYGEKRVYFDNGKEELFVGYLTEYSLPGMRTLFVEISNTKRCMIGKVDLPGDFIEV
                     RVNGFGLHDTQKIPLDDGYF"
BASE COUNT         1672 a         1531 c         1600 g         1618 t
ORIGIN      
        1 aatgtcgttg tggaaaccac atcagagatg cgaggtagtt atggaaattg atctcgaatt
       61 cggcgatgag aacaattggg ttggtatcac agctattgtt ctacttgtca tgatcgccct
      121 tgtaggactt gcctccatcg gtgaacgcat gacgcccgtt gacgaattgg ggcagccgcg
      181 agtgatgaac gcggatgact ggcgcgtcca tgaagcaacc cgcgcctata acgaggaaat
      241 ctcagtcttg cgtgatgata tccgtgaagt gtccctgctg ttacagaaat cgccaaatcc
      301 aatcgcggca caaattcttt acgaacgggt tgctggtcac gcaaataacg gacagtccac
      361 cacgcagatc gctcgtcagc ttactgtgac tgccgccgag agcgtacttg catggtcgaa
      421 tggattggtg gatcgagcgt cagccatgaa tgcggtgaac aaagccattg aggtattgaa
      481 ataatgacga tgcctgatat tgcctttcat gggcaacggt ctttatctga tttacttgaa
      541 gcagaacgta gtatgcgtgc tgccagcgat gaaacagtgc gcggtttacg agacgtgctg
      601 ctaagtgttg ccaatcgtct ggatggtaaa cgcatggaag cgttgaggca tgttgacccc
      661 aagataccag gaaattggtc agctaaacaa tggaaagagt ttgtcgatgc aattcctgcc
      721 cctgatgcct ggggtgcaat gacaaccgcg gcaatttcca tgagtaataa tgaggaaaac
      781 agcagatacg tggaagagtt aaaagcggaa atccagaatc tgatggtcga tctggaaaac
      841 gctaataccc ggatcgccga tatggaaaag cagcaggaag cgatattgct aaaaaccgtc
      901 aggaaaaatc cggttgaatt gaaaccaatg ccttccacca gacccaacgg atatgttttc
      961 cctccgttgg acgccctggt ccatgaagca gaatccaggc ttttattatt cccaaaccct
     1021 cccggcaagt tgatgaacgg gctggtaagc gtcgggtcgc gtgacggcaa gaatcgcctt
     1081 atctttcttc agcgtgtgtt tgtgtctcta ttcgccctgg gcgcccgtgg catatccaat
     1141 actctggaat tgaatcattt ggtcgcattg cctggcgagg cgcagcccaa ttcgggttcc
     1201 gtagggaagg tattgaacga tattgagaag aatggactga tcgcgatcga gcgatttgag
     1261 atgtccgaac caaagtctac attgaaggtt gcaaaactga ccgaaaaagg cgaggcgtta
     1321 ttcaaaggtc tgttcccaaa ggaaaaactt attgagtctg aagtatccgc ctccatgcag
     1381 tgggcacata acgatcgctt cagggcaact gccctcatgt attttgcagc gcttgctcga
     1441 aagcgaggct actatacaag catgaattca aatcaagaat ctgacctttg gttgggcagg
     1501 ggagaagagt ccaagtacac aattttggtg gattcgctta ccgacaacag atcgcaagca
     1561 ttggcaaagc tcaataatgg aaagacctgc atctgtgcca caaacaagtc catcatggaa
     1621 cgtgtcgctg gaaactgtgg tgtttcagga attggtggtg aagcgacgga tcttgaaaca
     1681 attctgacac acaagtatgt ggatatcact cccaacgacc cactttggcg tatgacatgg
     1741 tagatgggtg atccgcgaag gactgcctgt tttgcaggca tatcttcgca ggctacccag
     1801 cctgacagaa cctttacaag tgaatatgcc ggaaaaggaa ttcctccggc gcgatccgtg
     1861 tacgggttca ccaagacgtt tcttgcgtcc tggtggatca acgccgggac gcgctatttc
     1921 aatctatgga ggtattccaa tgttagctaa caaactgatg acccttgcgg gcatcccctg
     1981 gtccaccgtg gaagtggaac tggataagga actcccagcc gatgcgtatt cgcctgttcc
     2041 gggcggtgca agtttgacgg acatcgatcc cgcatttatg cgcgatgaat tcaaccgcct
     2101 ttttggcgtg tgcggtttcg ggtgggggta cgagtacgac gaagacaatg ttgtgcttga
     2161 tacggtaaag cgtggtcaag gaaccgcttt ctctgccatc atcaagaaat tgatcttctg
     2221 ggtcaagctt ctcgatgatg aaggcaagga agtgattttg aagatcgctt catctggcgg
     2281 ctcggtgaat gatgttcaag ggtacgcgct ctcgggtgcg atcaccaatg ccattgggaa
     2341 agcggcatcg aacatcggtt ttcaaaaatc cgtatatctt ggggcgcgtt cccataagaa
     2401 tgtgggtgcg aagtccccga aacctgcagc cacttctgcc gcacccccaa aggcgacgca
     2461 agccgtcaaa aagccagcag ctcctgccaa acctgcggca caggctccca agcctcagcc
     2521 aaagatacag gaaccgatcg acgatgagat catcgatgat gaacctgccg ctgccgtatc
     2581 tgaagcttcc aactttgtga tctccttcgg aaaccgcaaa ggacagaagc tcggcgatgt
     2641 ggatctgaaa tatatcgcct ggtatgccaa cgacatgaag acggaaggtg atcccgagaa
     2701 ggtcgcgttg caaaacgctg ccaaagagtt gctaaagatt cgctccaacg gtcacgcagt
     2761 tccggcataa accgcaaggc tctgtttggg agatggcaac tccatctccc aaatacgcag
     2821 gttatcaggc acacatttgc ctgtttccgc agtcgcacat taacaattca gaaacaagtg
     2881 gttcgccatt acatgcgaac ctatgctatt tggagcatac ccaggggtac aagggagtat
     2941 gcaccgaata agatcattta ctacagggac atgctcgtct gctcctgata attaatttgg
     3001 aggtttatat ggcaacagcc aaaagaaaag catctcagga tcgacgtagg tcgttcggtg
     3061 agcgttttga cctaaatacg gtatctctga ctggtccggt tatagcagtt tggggacgca
     3121 acggcgatgt gtttgttcgc atccaatccc caaacctgct gtctctgcct ggtccacaaa
     3181 gttatgtgaa catagtcatt cctggcggaa tgattgacgg cgacccggtg actctgcaaa
     3241 agggcgatct tctgcgtgtc actgggtatg tccgccagtc gaattttcag gaatccttgc
     3301 gtctcatcct agaaggtgca gggaagcgaa ctttctttga ccgatcagaa atacttcagg
     3361 aagatcgttt ggcgtggaag agaatttaca ttaaacgccg taattcaatc gttcaagctc
     3421 tcaaggttga agttttagtt gctcctgccg agtcgcaaaa ctttgttgac ctggagggca
     3481 ttgtaagcga agcttggttg tatccgcgtg atgatcacga agatctattt ttccgtattg
     3541 cgatttacga tgaacacact attgaaactg ccaatgaagg aaattttgga aggaagaagc
     3601 ggtacgctca ttacatcaac atccttctcc cagagcagcg tccagtttca tcgccggatg
     3661 aacgaagaat aagtatcgat ataaaacagc gcgttcgcgt ccgtggtgaa ctgcggtgta
     3721 cctggagcgt ggaaagcgtg catgacttgt tattctcgac cgggaggtcg gatattgctg
     3781 gattactcca gcgctcaggt gctgttagcg acgaactaaa aaccattaac acgcaattgg
     3841 aaacactgac agtggttgca aacgcgcttg tcctgtacag tcgttgacat aaatatgtaa
     3901 acatcaagca cctctttgtc gttgagcggc catttgggtt cgtgacccga aagaggtcca
     3961 gtctcgatag agacaccagt aaattttctc ctcctctcct tggtttcccg ccccggcaca
     4021 gcgcggatgt ttgttcctac ctgcaaacat ctgccgcgtg cctcggggcg tgcctggatg
     4081 atgtcacatt ccatcctgca cacaagtcca acgaaggcgg ttgatatttg ctggtagagg
     4141 agattcaaaa aatctcctct cattcatcct gcgcgactca gttacgcatt actttgtaac
     4201 ggaattgagt agggcaaaac gagacgttta cgcctcgtgc cgggatcgtc acccggcagg
     4261 atgactttgt tgggtatgat gccttcgaaa catcacgcac aacaagaagt cttgaaatag
     4321 gtttggaaat accactgctg aatgactgag acattgcctg gcacattgcc ttgtcaatgt
     4381 ccattcattc tctttgagtg tttctcttgc ctttcttcct gactttccgc acttcggcta
     4441 accgccgagt tgctcctcga ttttattgtg tcaggttggg ctatctgcct cacctgtcac
     4501 aaaactcaat catctcttaa ggagcatcta tgtactacag aaaattgttc gtcttaatca
     4561 cggttctatc gcttgcgctc tccgcgtgca gcgttataaa agcagaaccg accatgtccc
     4621 tcgaagatat tcaggcgact gcccagtcag ttgcccttac ctccgtggcg caaactgaaa
     4681 tggcaaaacc tactgaaccg cccactcaga caccaatccc acctacgccc attgtggaag
     4741 taaccgagga acctgtaact gaagtggtgc agatacccca ggaagttgct ccgattgtgc
     4801 ctcctccaac tgaacctgcc cctgtggtta tagtgcaacc cacggatgcg tccagtacag
     4861 gggatgggca atgcgatcct tacctaacag ccggtgataa gggagcgcgc acttcagtaa
     4921 cgatcaagaa ccaatctgct ggtccaatga cgttgtcgat ctatctgtac ccaactaaac
     4981 tcaatgcctg tggatcgaca agtgtgacac tggaaagtaa atcagaaaca acgatggagt
     5041 tgatcgaagg atgttattac ctgcatgcct ggatcaacgg caaaaaacca tccaacgcgt
     5101 ctgctgaata ctgcttcaaa gtggcagacg gaccgcgcaa gtggatcgtc actgcagagg
     5161 ttattgaaca aagataatta aatttagtaa ttcgtactca tcctcccgcg cacagtcaag
     5221 gctgtccaca ggctcaagac ctggcgggag ttttcttttt gtagctgatc caggtcacca
     5281 acctgtttta gtgttcgcaa ctcatcccat tttatggatc gaggggcgga catgaaaacg
     5341 gtcatgaagc gccccttggt caaaggagct tctccatgac cgcttttttc aatatgttcc
     5401 aaggcaacaa acaaaaagac gctgaaacgc ttgagaaaat tcttccaaaa ctttctcctg
     5461 catcgcagga agaggtcaaa agatttcttg acggatcatt ggcagagtta tccaactttg
     5521 ccaaaactga agcctcaatt gcctccctca aaggaggtgt tgcctaggac gctactcgcg
     5581 tccaccatgc cagtgacatc ctcactggca atcttgcatt tatgacttct aatagaagtt
     5641 tatttcttcc gctgaagcac cgccattttg gaatgtgcaa gctgtgatca ggttcgtgac
     5701 ctgacggaag atatccttcc agcacacggg aatggacgcg cgaagtacgc ttgaggccgt
     5761 cgcctcgcct ccctctgtat ctctcaggca gaggggaagg taattgtcca ctgggagcgc
     5821 agaccggggg agagtttgct tgtgatgcgg caacctcccc taggtcaaac ctaaaaaggc
     5881 ggcgtatgcg taattctaag aaaactttgg aaccttctga tctaaagaga caggttcaat
     5941 ctgctataaa gatggctttc caaataacgt ttctgttcct gggtggcatt ttattgatgg
     6001 tttgtacata cctgtatctt gcttttcttg aaatatacct cggatgaggc agggcatgaa
     6061 aactttattt cgtaaacgga tgacccagat actgtcgctt gtgctttctg tgtactgcgc
     6121 aacagttctg atctgtacgg ataacaagag cttgcatctt ttctgcgacg gagacatgtt
     6181 tatcttgatt gatcattacg gcgagaagcg ggtgtatttc gacaatggta aagaagaatt
     6241 atttgttggt tatcttactg aatatagcct tccaggaatg cgaactttgt ttgtcgaaat
     6301 atcaaatacc aaacgctgta tgattgggaa ggtggacctt cccggcgatt ttattgaggt
     6361 ccgtgtaaat ggttttggac ttcacgatac ccaaaaaatt cccctcgatg atggttactt
     6421 t
//
LOCUS       DAOREF010000032         6314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6470_length_6314_cov_4.593386, whole genome shotgun sequence.
ACCESSION   DAOREF010000032 DAOREF010000000
VERSION     DAOREF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_6314_cov_4.593386"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /gene="tsaD"
                     /locus_tag="PKI33_01185"
     CDS             <1..995
                     /gene="tsaD"
                     /locus_tag="PKI33_01185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNM35639.1"
                     /translation="ETSCDETACAILENGRALLSSVVASQMEIHARYGGVFPEVASRQ
                     HVLSIMPVVQQALAQARLSLSDIDAIAVTQGPGLAGSLIVGMNMAKGMALALDKPLVG
                     VNHLEGHIYSSWVYEAGDHKPPAPQFPLMALLVSGGHTELNLMTDHLTYQRLGATLDD
                     AAGEAFDKVARLLDLPYPGGPSIQREAEEGDPNRFNFPRAWLDGTWNFSFSGVKTSVL
                     YEVNDLKKRSNNIPVHDLAASFQKAVVDVLFKKTMDAARKYGAKEILIAGGVSANRAL
                     RQAFKAQTEFPVHIPPLALCTDNAAMIGAAGYFRYALGHASAMDIDVLPAWPLS"
     gene            complement(1189..2361)
                     /locus_tag="PKI33_01190"
     CDS             complement(1189..2361)
                     /locus_tag="PKI33_01190"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNM35640.1"
                     /translation="MKALFENLWSRISDLAYIETDNETIQRKGFLLSLYHLLILGILS
                     YTTINTLLYLLFYPSLEYGIYLLEDVVMFGLFYVFWRLTKRGNISLIGHISITLTILL
                     AIVFSDPKYLEYSMVIFALPIAISSFTLRPAMSFAYAFLISFVYILYSFIQSYVWEYN
                     LTPIVALFALAVTTWVISQQLENAISKNEKLVKDQQKTYDELKDAYETTLEGWSKALE
                     IRDQETQGHSKRVSDLTLRIARRMGFNDEQLVHVSRGVLLHDIGKIGIPDEILHKPGP
                     LNEKETRIMRLHPQISVDLLSPIEYLKPALSIPKYHHEKWDGSGYPHGLSGEMIPLEA
                     RIFAIVDVYDALSHDRPYRKAWSKEKAIEHIKSETGKHFDPAVTLIFLEEIEKKKR"
     gene            complement(2396..3256)
                     /gene="prmC"
                     /locus_tag="PKI33_01195"
     CDS             complement(2396..3256)
                     /gene="prmC"
                     /locus_tag="PKI33_01195"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNM35641.1"
                     /translation="MTQTLGDYLIHKIPKLPDDLTQQDAQLLLAHVIGRSRTWLLAHL
                     DAPLSAPLLDSANQAFARLEAGEPLPYILGHWEFFGMDFDITPDVLIPRPETETMVEK
                     AIKWLSASGERRTVADIGTGSGIIATSIAMHVPNANILATDISRAALKVAKHNAEKFN
                     VQHRIDFLECDLLPEHMDPLPTDRHFDLICANLPYIPTQTMRRLPIYGREPTLALDGG
                     EDGLDIYRRLLNVAPNWLAPHGMILLEIEATQGIKALNLATDIFSESSISLHQDFAGH
                     DRLLEIIPRL"
     gene            complement(3253..4104)
                     /locus_tag="PKI33_01200"
     CDS             complement(3253..4104)
                     /locus_tag="PKI33_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNM35642.1"
                     /translation="MRKISLPRQIATQLIFLLVGLFILIPVWSTFRLAFDGSLRGRPT
                     EFTWLPKEFSAEAFTKVLDRPYQSVEFNILLRNSMIVSIGAAVLAVILGITLAYGFAR
                     FRFPAKQTGLFVLLLAALLPPIAFATPLYIILSLLKIRTTLLGLVIVYAAFAMPFAIW
                     NMRATFAAIPKELEEAAYLDGANHYQTFLNISLPISLPSISVTAMIAFLMGYTEFAIG
                     WLFVDKADNVTLSMAVYALVNGQFSGAQPWSYLGSLAIIMSIPVMVIFIFFQETLMER
                     LLFQETS"
     gene            complement(4097..6208)
                     /locus_tag="PKI33_01205"
     CDS             complement(4097..6208)
                     /locus_tag="PKI33_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM35643.1"
                     /translation="MKKFQLPLRILSIITVLIIAGMTRARAVQHLNIDFDEDDYTRAA
                     QEFAHLIRTSNWSGFLEMNYRPEHPPLAKIIMGISLLTAPEKDLIADRPTSAEPNKYL
                     PRVILKPERTVNAVFGWLTVALLTIVNPLAGLSLATHSITVKYNSQAMLEALPALTSF
                     CMVLAYLQFKKNHNTRWLITSAVFLGLTAASKYLYCVIALAILADWFLTSKEQEELKS
                     FFKQALIWGTLGILIFFVTNPFLWANPIQRLRESVLYHAGYSSSSEEVKDAGFPVWQP
                     VQWLVTSAADWEEDAFFTGPDLLIAILAAFGFARLWKKERLYVLWFGFALLFLLLWST
                     KWPQYILILTVPLSLAAAEGLTQIWQNFLEGFKAPKTVYNKKESRQAIPWLVPGLIVF
                     TVLTLLPFFFQLGVSLTDFSSQSIRDGFQGGIWREVHGGLTGQITPTSPEAAYNSNKV
                     QYIGFQAYQPVFEYIVSVNKIFVFNILWMVVSTSIQVLLGLGLAILLNQRGVKLGKFW
                     QTLFILPWAIPEFIGALMWLNIFVPEIGWLSLAAKKYGDAFPLQSLIGWQSTPSLWFL
                     VLLIPAIWYGTPLIMLASNASLKTIPKEIFEAASLDGANSWQILQHMVLPLIRPVLLP
                     AIIIRGIYAFNQFYLFQAMPFSEGTLANFSYNVFNPSSRFGGGQFAISAVINIITVLI
                     LIVLVSAFNRRMQTREEFINA"
BASE COUNT         1747 a         1327 c         1624 g         1616 t
ORIGIN      
        1 tcgaaacttc ttgtgatgaa actgcctgtg ccattctcga aaatggacgg gctttacttt
       61 cttccgtagt tgcctcccaa atggagatcc atgctcgtta tggcggcgtg ttccctgaag
      121 ttgcatctcg ccagcatgtg ttgagcatca tgccggtcgt gcagcaagcg ttggcacaag
      181 ctcggctatc tctcagtgat attgatgcca tcgcagtcac gcaagggccc ggtcttgccg
      241 gttcactgat cgtgggcatg aacatggcga aaggcatggc acttgcgttg gataagccgt
      301 tggtgggtgt caaccatctt gaaggacata tttattcttc atgggtgtat gaggctggag
      361 atcacaaacc gccagcaccg caattcccat tgatggcatt gctcgtttcc ggcgggcaca
      421 ccgagttaaa tcttatgacc gaccatctga cctatcagcg gcttggtgcg actctcgatg
      481 acgcggcagg ggaggcgttc gataaagtgg cgcgcttgct ggatttgcca taccccggtg
      541 gaccgtctat tcagcgtgaa gcagaagaag gcgacccgaa ccgcttcaac ttccctcgcg
      601 cctggctcga tggaacctgg aatttctcct tcagtggagt gaaaacctcg gtattgtatg
      661 aagtgaatga tttgaaaaaa cgcagtaata acatccctgt ccatgatttg gctgcttcat
      721 ttcaaaaagc cgttgtggat gtgttgttca aaaagaccat ggatgctgct cgtaaatatg
      781 gcgcgaaaga aatattgatc gctggtggcg tctctgccaa tcgtgccttg cgccaagcct
      841 ttaaggcaca aacggaattt cctgttcaca ttccacctct tgcactttgt acagacaacg
      901 cagccatgat cggggcggcg ggatacttcc gctatgcatt ggggcacgct tctgccatgg
      961 atatagacgt tttgcccgct tggccgcttt cgtaattgaa cgtaggctct aaaatgaatt
     1021 gccccattaa aggaccaaac ccccgttcgt ttgcccaaac gattgggggt ttgtcatcag
     1081 gagaatgcaa ctattattat atttgggcag tttaatggtt cctgaacctt gattaaggat
     1141 tggattagag gattaaggga ggaaagatta aattttgtga agatattttt atctcttctt
     1201 tttttcgatt tcttctaaaa agataagagt cacagccggg tcgaaatgtt tccctgtttc
     1261 tgattttata tgctcaatcg ccttttcttt tgaccaagct tttcggtagg gacggtcatg
     1321 ggaaagcgca tcgtacacat ccacaatagc aaagatgcga gcttctaagg gaatcatttc
     1381 tcctgatagc ccatggggat atccgctacc gtcccacttt tcatggtgat attttggaat
     1441 ggacaaagca ggtttgagat attcaatagg ggagagcaaa tccacagata tttgcggatg
     1501 caacctcatg atgcgagtct ctttctcgtt tagcggacct ggcttatgca aaatctcatc
     1561 gggaattccg atctttccga tatcgtgtaa caaaacccct ctagatacat gtacaagctg
     1621 ttcgtcattg aatcccatac ggcgagcaat gcgcagcgtg aggtctgaaa cccgtttgga
     1681 atgaccttgg gtctcctggt ctcgaatttc aagcgctttc gaccagcctt ctaatgttgt
     1741 ttcataagca tcttttagct catcatatgt cttttgctga tcttttacta gtttctcatt
     1801 tttgctgatt gcattttcaa gttgctgtga aatgacccag gttgttaccg caagagcaaa
     1861 caaagcaaca ataggtgtga ggttgtactc ccaaacatag ctttggataa aagaatacag
     1921 gatgtaaaca aaactaatta agaaagcgta tgcaaacgac atggcaggtc gtaacgtaaa
     1981 gctggagatt gcaatcggca atgcaaagat aaccattgag tattcgagat attttgggtc
     2041 actaaataca atagcaagca aaatggtcaa cgtgatcgaa atatgaccga tgagagaaat
     2101 gtttcctctt ttggttagac gccaaaaaac ataaaacaac ccgaacataa ccacatcttc
     2161 aagtagatag attccatatt cgaggcttgg atagaaaaga agataaagca aagtgttgat
     2221 ggtggtgtaa ctcaaaatcc ccaaaatcag taggtgatat aaagaaagca aaaagccctt
     2281 tctttgtatg gtctcgttat ctgtctcaat atatgccaag tctgaaatac gcgaccatag
     2341 gttttcaaag agtgctttca ttaagatatg cctagatttg attatatctt ttgttttaga
     2401 ggcgtggaat aatttcgagc aaacggtcat gacctgcgaa gtcttggtgc agtgaaattg
     2461 aagattcaga aaaaatgtct gttgcgaggt tcaaggcttt aatgccttgc gttgcttcaa
     2521 tttcaagcag aatcatgcca tgaggggcaa gccaattcgg tgcaacgttc aaaagacggc
     2581 gataaatatc taaaccatct tcgccaccat cgagagccag tgtgggttct cgcccataga
     2641 tgggaagcct gcgcatggtt tgggtgggga tataaggcag gttggcgcag atcaaatcaa
     2701 aatggcggtc ggtgggtagt gggtccatgt gttcgggcag caagtcacat tccaaaaaat
     2761 caatgcgatg ctggacgtta aacttttcgg cgttatgttt tgcgaccttg agcgccgcac
     2821 gagaaatatc ggtggctaga atgtttgcat tcgggacatg catggcaatg gatgttgcga
     2881 taatgccaga acctgttcca atatcggcga cggttcttct ctcgcccgaa gcggatagcc
     2941 acttaatggc tttttctacc atcgtttctg tttctggtct cgggatgagt acatcgggtg
     3001 tgatgtcgaa gtccatgccg aagaattccc agtgaccgag gatgtacgga agcggttcac
     3061 ccgcttcgag ccgggcgaag gcttggttcg ctgaatcaag tagcggcgcg gaaagaggcg
     3121 cgtcgagatg ggcgagcagc catgtgcgtg aacgtccgat cacatgcgca agcaagagtt
     3181 gcgcatcttg ttgggtcagg tcatctggca gttttggaat tttgtgaatg agataatcgc
     3241 ccagagtttg ggtcatgagg tttcctgaaa gagtaggcgt tccatgagag tttcttggaa
     3301 aaagatgaaa atgaccatca ctggaatgga catgatgatg gcgagtgagc caagatagct
     3361 ccatggttgt gcgccagaga attgtccgtt gacgagggcg tagactgcca tggaaagtgt
     3421 gacgttatcg gctttatcta caaagagcca gccaatggcg aattcggtgt atcccatgag
     3481 aaaggcgatc atggcggtaa cagaaataga cggcaatgag atgggcaggc tgatgttgag
     3541 gaaggtttgg tagtgattcg ctccgtcgag gtaggcggct tcttctaatt cttttggaat
     3601 ggcggcgaaa gtggcacgca tgttccaaat ggcaaagggc atggcgaacg ccgcatagac
     3661 gatgaccaag ccgagcaaag ttgtgcgaat cttcagcaga cttaagatga tgtacaacgg
     3721 agtggcaaat gcaatggggg gtaacaatgc cgctagcaat agaacgaaca agccggtctg
     3781 cttggcagga aagcgaaaac gcgcaaaccc ataggcgagt gtgatgccca atatcactgc
     3841 aaggactgcc gcaccaatcg aaacgatcat actgttgcga agtaggatgt tgaactctac
     3901 ggattgatag gggcgatcta atactttcgt gaaggcttca gcggagaatt ctttgggcag
     3961 ccatgtaaac tcggtaggac gtccgcgcag ggagccatcg aaggcgagac ggaaggtgct
     4021 ccagaccggg atgaggatga agagaccgac caacaagaag atgagttggg ttgcgatctg
     4081 acgaggcagg gatattttac gcattgataa actcctcgcg agtttgcatg cggcgattga
     4141 aggcgctgac caagacgatc aggattagga ccgtgatgat gttgataacg gcagagattg
     4201 caaattgacc tccgccaaat ctgctggaag gattgaatac attgtaagaa aagtttgcca
     4261 gagtaccttc gctgaatggc atagcttgaa atagatagaa ctgattgaag gcataaatac
     4321 cgcgtatgat aatggcaggt aacaaaacag gtcttatcaa cggcaagacc atatgctgta
     4381 agatttgcca actattggct ccatcaagag aggcggcttc aaagatttct ttcggtatgg
     4441 ttttgaggct cgcatttgaa gcgagcataa tcaaaggtgt tccgtaccat atggcaggaa
     4501 taagcaaaac caagaaccaa agactgggtg tgctttgcca accaattaga gactgcagtg
     4561 gaaaggcatc gccatacttt ttggcggcaa ggcttaacca cccaatttct ggcacaaaga
     4621 tgttcagcca catcaaagcg ccaataaatt caggaattgc ccaaggcaga atgaaaagag
     4681 tctgccagaa cttgccaagt tttaccccgc gttggttaag caaaatagca agccctaacc
     4741 ctaaaaggac ttggatactc gtagaaacca ccatccaaag gatgttgaaa acaaaaattt
     4801 tatttactga aactatatac tcgaatacag gttgataagc ttggaaacca atatattgaa
     4861 ctttatttga attgtaagca gcttcaggag atgttggtgt aatttgtcct gtaagcccgc
     4921 catgtacttc acgccagatg ccgccttgga atccatcacg gatggactgg ctgctaaagt
     4981 ctgtaagtga aacgccaagt tgaaagaaaa acggaagcag ggttaaaaca gtaaacacaa
     5041 tcaagcccgg cacaagccag ggaatagctt ggcgtgattc ttttttgttg tacacagttt
     5101 ttggggcttt gaacccttca agaaagtttt gccatatctg tgtgagtcct tccgcggcgg
     5161 cgagcgaaag agggacagtt agtatcagaa tgtattgcgg ccacttggta gaccataaaa
     5221 gcaaaaacaa caaggcaaag ccgaaccaca atacatacaa gcgctctttc ttccataatc
     5281 gtgcaaaccc aaatgcggca aggattgcaa tcagaagatc tggtcctgta aagaaagcat
     5341 cttcttccca gtctgcggcg ctggttacca accattggac aggttgccaa acgggaaacc
     5401 cagcatcttt gacttcttct gaacttgaag aatagccagc atgataaaga accgattcac
     5461 gcagtctttg aattggattt gcccagagga atggattcgt cacgaagaag atgagaatcc
     5521 ctaacgtgcc ccagatcaag gcttgcttga agaatgattt gagctcttct tgttctttcg
     5581 atgtcagaaa ccaatccgcg agaatagcga gagcgatgac gcaataaaga tactttgagg
     5641 cggcagtaag accaagaaag acagccgatg taattaacca gcgggtattg tgattctttt
     5701 tgaattgtaa gtacgccaat accatgcaga aactggtcaa tgctggcaat gcctctagca
     5761 tggcttgcga attgtattta acagtgatgc tatgcgtagc gagacttaac ccagccaatg
     5821 ggttgacgat tgtcagcaat gccacggtta gccagccgaa gacggcgttg accgtccgct
     5881 cgggcttgag gatgacacgc ggcaaatatt tattgggttc agctgaagtg gggcgatctg
     5941 cgatcaggtc tttttctggg gcagtgagca agctaatgcc catgatgatc ttcgcgagag
     6001 ggggatgctc ggggcgatag ttcatttcca gaaagccgga ccagtttgag gtgcggatga
     6061 gatgcgcaaa ttcctgagcg gcacgtgtgt agtcgtcttc gtcaaagtca atgttgagat
     6121 gttgaactgc gcgggcacga gtcatcccag cgataataag gacagtaata atgctaagga
     6181 tgcgaagggg gagttggaat ttcttcatgg cgctaaattg tatcctctca atcctcgtga
     6241 attgtaatac tgcgaggtgg attattgaaa acaggctgga gtttctccag cctgtttcgc
     6301 gaattttatt cttc
//
LOCUS       DAOREF010000033         6313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6473_length_6313_cov_3.830617, whole genome shotgun sequence.
ACCESSION   DAOREF010000033 DAOREF010000000
VERSION     DAOREF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_6313_cov_3.830617"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4646)
                     /locus_tag="PKI33_01210"
     CDS             complement(<1..4646)
                     /locus_tag="PKI33_01210"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM35644.1"
                     /translation="MNFIYTPYSIPLIAAAGISIFVAIYAWRHRSSPGAPALTLLAAA
                     IAQWILGYILEISSADLNTIYIWGVSQYIGIAFAPYAWLIFAIDFAGYSKLLTRRFYV
                     LTAIFPVITVLLAWTTRWHGLVWAKYDISLVEGFSALATQKGYWFYAHTAYSYLMLLI
                     GTILLVRALVRLQGLYRSQVIAMLIAVFAPWIGNVLYLTGNSPIPYLDLTPFSFTVTV
                     AALAWAIFGFHLVDITPIARDLVIDSLQEGVIVLDANDRVADINSSAARIFGIQISHA
                     IGTLASELFTPWQSLRERLREDEDWVQEFSNGVGQAERQYEAAISHIQDAQGRNAGRV
                     MIVREVLSSSTRRRPASSSTEAASVSDSVDTTIETPKWLDAYPWLKKIFVYFYTPINT
                     DLVVPPNISPTWYLARERSFTIILRVGALAGTIAYFFTLPVMRVASRFSISSLFFGIV
                     IVLLWFLGGGRKIGFETRANVFLVLIYFMSVNETIGYGFSVECFLFFTAFVTISTLLT
                     GRRGLRLATLTSFITLVLFAWLIGGGYYIPLNLTVTTLRPQSLAIGFTNLFVFLATSS
                     AIANATIVLMENLNSAWRKESQTSNLLQQERDLLEQRVEERTHDLQEAESKFRTLVEQ
                     LPAVLYRDDADQNGKNNYYSPQAERMLGYPMSVWEKDSMLWHSILHPDDKERAIETIN
                     ETLSNGHSLSEYRLFDSDGRVVWVRDEAILVRESSGKPLFVQGIMQDITEIKKAEEEI
                     RKLSRAIEQSGNSIIITDTNGDIEYVNPTFEKITGYSFNEIKGQNPRILKSGRQRAEF
                     YDKLWATISSGNVWHGVFHNKRKDGTLYWENATIAPVMDATGRVTNYVAIKEDITARR
                     EADEQLRKLSQAVEQSANTVIIMDREGVIEYVNPTFSLITGYSSEDALGKTPERLLNP
                     ASKLDNFRNQEWWMTVNAGNTWRGEFRNYRKDGQVFWESASIAPVMDSNGAVTNFIEI
                     KQDVTEQRKLQDQLQKRNEYLSILHQVTLDLLNRRDVEDLLQVIVDRASVLLDATFSE
                     LMLEEDGYLVVKAFTANQPNLKGDRVGREQAKLSWQAFDTKEPVVLEDYATWEFRRDV
                     YDVNPIHATADFPVMTGETCIGVLALGRDQAGYAFTNEQIQTGILFARLVALVLDNVN
                     LYDSALREIAERERAQISLQRSNEQQHIIVSLLRVGLENMTLDQILGNILDGILSVGW
                     MKLVPKAGIFLVDKETQKLRLHTHRNFSPPLLNLCAQVDMGRCMCGRAAMQKQILFSD
                     CLDERHEIRYEGIEEHGHYNVPILQGKEVLGIIVLYLPHGYQRSTDDETFLLAIADAI
                     SGVIGRKRVEALLQGSEILFRQIVENASDIIYRTDMQGMFTYANPAALNMMGFTSEQE
                     VLGKNYLELTTPEARNRLKRVYDHQYLSRTNNTYYEFPAISMKGEIIWVGQNVQLIWD
                     GDKIVGFQALARNITQLRQAQEALLLARDQALEASRFKSQLVSRVSHELRTPLGGILG
                     FAELLKEGSFGSLNGSQMTAISNIVESTNFLTDTVNDLLDQAQIEARSINLHNA"
     gene            complement(4730..5593)
                     /locus_tag="PKI33_01215"
     CDS             complement(4730..5593)
                     /locus_tag="PKI33_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35645.1"
                     /translation="MLKLTRPLHLFLAALTYLLGASIPSYLGKPFLLAPFLMGLAFVL
                     LMQTSMSFLREVFRPHNEPLVEDETPQKKETLRNNLLYISVGMIGTAAVIAFITHLNS
                     PLALPAFLFLVSSLVLTLVYAIPPFHLVNRGFGELILSIQIAYIAPSIAFLFQADETS
                     RLLTTLLLPLTVLALAYFLILNFTTFPEDQKYERGTLLRRLGWERAVPLHHSLIAFAY
                     VCFALAPLFGFAFNLVVPAFLTLPFAIFQVFQLRSVANGNPPNWRLLTSTALAVFGLT
                     TYFLTLTFWLR"
     gene            complement(5595..>6313)
                     /locus_tag="PKI33_01220"
     CDS             complement(5595..>6313)
                     /locus_tag="PKI33_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35646.1"
                     /translation="YLIYSVYLDSILFLRKKLFVLLPYYVLLIVTSVFEKYFNSNFLL
                     KDFISFFIEFFLLIFLEIFTTVLVCKHTIFSSHEYPFWTMLKRYYERGLFLFVSTLFV
                     LILFGVVLAIFAPAIGKFLYTLSTISCMFICTLSLQYMVVYNKDSIRDSLKGGISSLY
                     KDFIFYFLVLLVGILINKFPSLLFPSSWLFLPILPIAEIEFILNPVAWLSWLIYPVLN
                     SLQFISITLAFIYKHNKWNS"
BASE COUNT         2008 a         1416 c         1339 g         1550 t
ORIGIN      
        1 taagcgttgt gtaaattaat cgatctggct tcaatctgag cctggtctaa caagtcgttg
       61 actgtatctg ttaagaagtt ggtactttca acaatatttg aaatggctgt catttggctg
      121 ccattgagag agccaaatga gccttcttta agcagttcgg caaaacctaa aatgccacca
      181 agaggggttc gcagttcatg gctcacacgt gagaccaatt ggctcttgaa acggctggct
      241 tccaatgcct ggtcacgcgc gagaagcaag gcttcttgag cctgcctcaa ctgagtgata
      301 tttctcgcca atgcctggaa gcccacaatc ttatcaccat cccaaattaa ctgcacattc
      361 tgccccaccc agataatttc acccttcata gatatggcag gaaattcata ataggtatta
      421 ttcgttctac tgaggtactg atggtcatac acacgcttta gacggttacg tgcttctggg
      481 gtggttaatt caagataatt tttaccaagc acttcctgct cacttgtgaa acccatcatg
      541 ttaagggctg ccgggttggc atatgtaaac atcccttgca tatctgttcg gtagataata
      601 tcactggcat tttctacaat ctgacggaac aggatttcgc tcccttgcaa caaagcttca
      661 acacgcttac gcccaatcac gccagagata gcatctgcaa ttgcaagcaa aaatgtctca
      721 tcatcggtac tgcgttgata tccatgtggc agatacaaga caattatgcc caatacttcc
      781 ttgccctgca aaatcggaac gttataatgc ccgtgctcct ctatgccttc ataacgaatc
      841 tcgtgccttt catccaaaca atctgaaaac aggatctgtt tctgcatggc agcacgccca
      901 cacatgcagc gccccatatc cacctgagca cataaattca gcaaaggagg ggaaaagttg
      961 cgatgtgtgt gcaggcgcaa tttttgagtc tctttgtcaa cgagaaagat gcccgctttc
     1021 ggaacaagtt tcatccaccc tacagaaaga attccatcca gaatatttcc cagtatttgg
     1081 tcaagggtca tgttttcaag cccaacgcgt aaaagagata cgataatatg ctgttgctca
     1141 ttggaccgtt gcagggaaat ttgagcacgt tcacgctcag caatttctcg caaggcggaa
     1201 tcatacaaat taacgttatc taacacaagg gctacaaggc gggcgaaaag aatgcctgtc
     1261 tgaatttgtt cattcgtaaa tgcatatcca gcttggtcac gccccaaggc gagaacgccg
     1321 atacatgtct caccagtcat aacaggaaaa tctgctgtag catgtatcgg gtttacatca
     1381 tatacgtcgc gccgaaattc ccaggtggca taatcttcaa gtacaacggg ttcctttgtg
     1441 tcaaaagcct gccacgaaag ttttgcctgc tcacgcccaa cccggtcgcc ctttaagttt
     1501 ggttggttgg cggtaaaagc tttcaccacc aaatacccat cctcttccag catgagctcg
     1561 ctaaacgttg catccagcag aacagatgcg cggtctacga tcacttggag cagatcttcc
     1621 acatcacgac ggttcaaaag atccaaggta acttggtgca aaattgataa atattcattc
     1681 cgcttctgca attggtcttg cagctttcgc tgttcagtaa cgtcttgttt gatctcaata
     1741 aagttggtca cagcgccatt cgaatccatc acgggggcaa tactggctga ttcccaaaat
     1801 acctgcccat ctttgcgata gttgcggaac tcacctcgcc aagtattccc agcattgacc
     1861 gtcatccacc actcttggtt tcggaaatta tcaagtttcg aggctggatt cagcagtctc
     1921 tcaggggttt tgcctaaggc atcttcagaa gagtatcctg tgatcagtga gaaagtgggg
     1981 ttcacatatt ctataacacc ttcacggtcc atgatgatga ccgtattagc actctgttct
     2041 actgcctgtg acaacttgcg caactgctca tccgcttctc ttcgagcagt aatatcttct
     2101 ttaattgcca cataatttgt caccctaccc gttgcatcca taacaggcgc aatggtggca
     2161 ttctcccaat acagggttcc atccttgcgt ttgttatgaa acactccatg ccaaacattt
     2221 ccagaggaaa ttgttgccca caacttgtca taaaattctg cacgttgtct accagatttc
     2281 aagatcctgg ggttttgccc cttgatttca ttaaaagaat agcctgttat tttttcaaac
     2341 gtagggttca catattcaat atcgccattc gtatcggtaa tgatgatcga gtttccgctt
     2401 tgctcaatag cacgtgataa ttttcgaatc tcttcttctg cctttttgat ctccgtaata
     2461 tcctgcataa tgccttgaac aaacaaaggc tttccagacg attcacgcac caagatggct
     2521 tcatcacgaa cccaaacaac ccgaccatca ctgtcaaaca gacgatactc cgagagggaa
     2581 tgtccattag agagcgtctc attaatagtc tcaatggcac gttctttatc gtcagggtga
     2641 agaatgctat gccaaagcat ggaatccttc tcccaaacag acatggggta ccctagcatt
     2701 ctctcggctt gtgggctgta gtagttgttc tttccgtttt gatctgcatc gtcgcggtac
     2761 aaaactgcag gtaactgttc aacaagggtt cggaatttag attctgcctc ttgcaaatca
     2821 tgtgttcgtt cttccacgcg ctgctcaaga aggtcacgtt cctgttgcaa taaatttgaa
     2881 gtttgtgatt ccttacgcca ggcagagttt aggttctcca tcaagacgat ggtggcgttt
     2941 gcaattgcag aagaagttgc caaaaacacg aataaattcg taaacccgat ggcaagcgat
     3001 tgcgggcgga gtgttgttac cgttagattc aggggaatat agtaaccgcc accaataagc
     3061 caggcgaata atacaagcgt gataaaactg gtcagggttg caagccgaag tccacgccgc
     3121 ccggttagaa gagtagaaat cgttacaaaa gcagtaaaga ataaaaaaca ttccactgag
     3181 aaaccatacc caatggtttc attaaccgac ataaaataga ttagaactaa aaagacattt
     3241 gcacgtgttt caaagccgat ctttcgcccg cccccaagaa accataaaag gacaataaca
     3301 attccaaaga aaaggctgga tattgaaaaa cgcgaagcaa cacgcatgac aggcagagta
     3361 aaaaagtatg cgatggtccc tgccagtgca cctactcgca agataatggt aaaagaacgc
     3421 tcacgtgcca aataccatgt ggggctaata ttaggcggca ctaccaagtc tgtattgata
     3481 ggcgtataaa aatagacaaa aattttcttt agccatggat atgcatccaa ccacttcgga
     3541 gtttcaatcg tagtatccac gctatcagaa acagaagcag cctcggtgga agatgaggcg
     3601 ggtcttcgtc gcgtagagct agaaagcacc tcacggacaa tcatcaccct gccagcattt
     3661 ctcccctgtg catcctggat atgagagatc gccgcttcat actgacgttc agcctgtcca
     3721 actccatttg aaaattcctg cacccaatct tcatcttcgc gcaaacgctc gcgaagtgac
     3781 tgccagggtg taaacaactc agatgccagt gttccaatgg cgtgagaaat ttggatacca
     3841 aaaatacgag ccgcagacga gttaatatct gcaactctat cattggcgtc cagtacaatc
     3901 acaccttctt gaagggaatc gataaccaaa tctctagcaa tgggcgtgat atcaacaaga
     3961 tgaaatccaa agattgccca agccaatgcc gcaacggtga ctgtgaaaga aaaaggcgtg
     4021 agatcgagat atggaatagg gctgttgccc gttagataca aaacattccc gatccatggc
     4081 gcaaagactg caattagcat cgctataact tggcttcgat acaaaccttg taagcggacc
     4141 aaagcgcgca ccaacaagat cgttccaatc agaagcatca agtaggaata agcagtatgg
     4201 gcgtagaacc aataaccttt ttgagtggcg agcgcagaaa aaccttcaac aaggctaatg
     4261 tcgtatttcg cccaaaccaa accatgccag cgagtcgtcc atgccaaaag gacggtgatg
     4321 actggaaaga ttgctgttag aacataaaaa cgtcgtgtaa gaagttttga atagccggca
     4381 aaatcgatcg caaatattag ccatgcgtat ggtgcaaagg caattccaat gtactgactt
     4441 accccccaaa tgtaaatggt attcaaatct gcactggata tttccaaaat atatccaaga
     4501 atccactgag caattgcagc tgcaagaagg gtcaacgccg gggctccggg agaagagcga
     4561 tgcctccagg catagatggc tacaaaaata gaaatcccag ccgctgcgat caaaggtatg
     4621 gaataaggcg tgtaaatgaa attcatgggc gattcctttg gcaaaatcag cccaattata
     4681 tagtcatccc ctgctgttgg ttcattacaa agctattagg ttttagccct taccggagcc
     4741 agaaggtaag agttaggaaa tacgtggtca gcccaaagac ggcaagcgcc gtagatgtaa
     4801 gcaatctcca atttggaggg ttcccattcg caacagaccg aagttggaag acctgaaaaa
     4861 tggcaaaagg caaggtgaga aatgctggca caaccaagtt gaatgcaaaa ccaaaaagtg
     4921 gagccagagc aaagcagacg taggcaaaag caatcaggct atgatgcaaa gggacggctc
     4981 gctcccagcc aaggcggcgg agaagggtgc cacgctcata cttttgatct tctgggaatg
     5041 tcgtgaaatt gagaatcaga aaatatgcca atgctaaaac cgtgagcgga aggagtaagg
     5101 ttgtaagtaa acgagaagtt tcgtctgcct gaaatagaaa agcaatcgac ggagctatgt
     5161 aggcaatctg tattgaaaga atcaattcgc caaatccacg attgacaagg tgaaaaggcg
     5221 gaatggcgta tacaagagta agtaccaaag aagaaacaag aaagagaaac gccggaagcg
     5281 caagagggga attgagatgc gtgataaacg caataacagc cgcagtcccg atcattccaa
     5341 ctgaaatata aagcaagtta ttacgaagag tctctttttt ctgcggggtt tcgtcttcga
     5401 caagaggttc attatgcggg cggaacacct cgcgcaagaa tgacatgctg gtctgcataa
     5461 gaagtacaaa agcaagaccc atcaaaaacg gagcaagcag aaaaggtttg cctagataag
     5521 aaggaatact tgcgccgaga agataggtca gcgcagcaag aaaaagatgg agaggacgag
     5581 ttagttttag caatttaaga attccattta ttatgcttat aaataaatgc taaggttatt
     5641 gaaataaact gaagagaatt taatacagga taaattagcc agcttagcca agctacagga
     5701 ttaagtataa attcaatttc cgcaataggc aggattggaa gaaacagcca acttgaaggg
     5761 aaaagaaggg acggaaactt attaattaaa atacctacaa ggagaactaa aaaataaaat
     5821 ataaaatctt tatataaact agaaatccca cccttcaacg agtctcgaat agaatcctta
     5881 ttgtaaacaa ccatgtattg aagggataat gtgcaaataa acatacagct aattgttgag
     5941 agggtataca aaaatttccc gatagcagga gcgaaaatcg ctaaaacaac tccaaacagt
     6001 attaagacaa aaagagttga aacaaataaa aacaaccctc tttcgtaata acgcttcaac
     6061 atagtccaga atggatattc atgactagaa aatatcgtgt gtttgcaaac taacactgta
     6121 gtaaatattt caagaaaaat caaaagaaaa aactcaataa aaaaacttat aaaatccttc
     6181 agcaagaaat tagaattaaa atatttttca aaaaccgacg tcacaatcag caacacatag
     6241 taaggcaata aaacgaacaa tttttttctt aagaataata tcgagtctag gtaaacagaa
     6301 tagatcaaat aaa
//
LOCUS       DAOREF010000034         6206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6651_length_6206_cov_4.046659, whole genome shotgun sequence.
ACCESSION   DAOREF010000034 DAOREF010000000
VERSION     DAOREF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6651_length_6206_cov_4.046659"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKI33_01225"
     CDS             complement(<1..667)
                     /locus_tag="PKI33_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNM35647.1"
                     /translation="MPEQTNTWETQQKILVILAHPDDPEFFCGATLAKWAREGHSIQY
                     LLLTCGDKGFNPTTTPDMTPEKLCGLRHIEQAEAAKVIGAEPPLFMDLPDGYLVPDLN
                     LRRDVVREIRRHKPDILLTCDPQNLFALYGINHPDHRACGQIVLDAVFPAAGNVAYFP
                     ELMDEGFEPHMPKEVWCSLTNQPNTTIDVTEFWDIKLQAILQHKTQVQDAEKLIERFK
                     SRRA"
     gene            complement(651..1598)
                     /gene="ricT"
                     /locus_tag="PKI33_01230"
     CDS             complement(651..1598)
                     /gene="ricT"
                     /locus_tag="PKI33_01230"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNM35648.1"
                     /translation="MQTDVKNIIGVRFTPIGKIYHFDSSSLPDLKQGEHVIVDTTRGR
                     HLGEVVQILQEAPPKPEGGWKSVERRATPRDLLLQQSWRNKQTEAMINCRARASELGL
                     LETIKVVAAEYNYDGSRLAFLFSTEQEEKVDLKSLRKDMSDIYPNTHIEMRQIGPRDV
                     AKLIGGMGACGIETRCCSKFLTDFSPISIKMAKEQGISLTPDEITGMCGRLRCCLIYE
                     YEQYVEARKTLPKRNKRVVTPKGEGKVIDVMPMSDKVMVLIEIPNERPQHHTFLREEI
                     EPWDELEALRRKSEAPCDRHEGGGCTCGKAKNNKNARAN"
     gene            complement(1697..2800)
                     /locus_tag="PKI33_01235"
     CDS             complement(1697..2800)
                     /locus_tag="PKI33_01235"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNM35649.1"
                     /translation="MSATIKKIAISTGGGDAPGLNAVIRAAALSAIKRGWEVYGVLDG
                     FHGILSPEEYPDGGFMPLTRQRVRGITHLGGTILGTSNRNNPMKYPIQRPDGTIEEVD
                     RTDEIVRGFRLHHIDALITIGGDGSLAIANVLAQKGLRLISVPKTIDNDLDKTDVTFG
                     FDTAVGFATEALDRLHSTAASHGRVMVVEVMGRYAGWIALHAGVAGSADVILIPEHPY
                     DIHKVVEKIREREESGNKFTIVVVAEGASPKGGGVSVVEHEAGRAERLGGVGEKVAAE
                     IHAISGKETRVVVLGHLLRGGSPTSFDRLTALRFGAAAVRGLEEGQAGVMVALDPPHV
                     KYVPIAEAIGQMKMVPMESDIVLTARDLGISFG"
     gene            3015..4226
                     /locus_tag="PKI33_01240"
     CDS             3015..4226
                     /locus_tag="PKI33_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM35650.1"
                     /translation="MNKVVSIDKTALLRSAFNGLSENELEEMAALTEVRIYPPEHILC
                     REGEYEETFYIIAEGTALISKYIGDQEGDRVLRTVGKGDLVGEMALIQNAPRSATVRA
                     TSELIALEMDKDHFETMLGRSPHMALDIIRITLNRLRENDQMMISELQKSNKVLQQLD
                     RNKTEFIDVVAHELRTPLTVLKGYSNLLSASPEVKNHPTLRDVSEGIQSGADRIHLIV
                     NTILDVSRIDNNELTLRRSPILLKQVVLEVLSGLTKAASERTIELFHLPGADTPLIHG
                     DPALVHKALYHLVINAIKYTPNGGKVTIATRPSKLDNGEEGVLISVQDTGIGLDPEHH
                     ELVFEKFYQAGHAAIHSSGSTSFKGGGPGLGLAIVRGVARIHGGKAWVESTGEDEVNF
                     PGSTFYLWLPV"
     gene            complement(4278..5285)
                     /locus_tag="PKI33_01245"
     CDS             complement(4278..5285)
                     /locus_tag="PKI33_01245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM35651.1"
                     /translation="MSTMKNIQVKHAPAIAGLSFRGFAGESDYPKMLAIIEGSKQVDQ
                     VERTDSLEDVRRNYSHLNNCDPYTDMLFAEINGEVIGYQRVWWDQLNDGVTRTYSGIG
                     FLLPAWRRKGIGTAMFQHAEERLRQIAAAHTGPETKVFSMWANGTAQGLIALLEKQGY
                     QSTRYFFEMARDIHEPLPEVSLPSGLEVRPVTEVDYRAVYEAHTEAFRDHWGFNEQTF
                     EEFMHWAEEEDFNPGLWMVAWDGDQIAGQVLNFVSNAENREYHRKRGYTEGISVRRPW
                     RKRGLAKALLVKSIAMFKDQGMSETALGVDAENPSGALGLYQSLGYKEIHRNVIYRKP
                     L"
     gene            complement(5401..6039)
                     /locus_tag="PKI33_01250"
     CDS             complement(5401..6039)
                     /locus_tag="PKI33_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35652.1"
                     /translation="MNAVHALYDEGLQELLTGMLSLSTFAEAKEWSERAIYEIGQLNQ
                     NAREIDEEVFRHTQALEKLQYERSKKVLGGLLGVGKEEKELAQQIEELKTSKNGLSKA
                     IQQLQEMMDFTPRSLDEKHALLTELKMRKRRLQEEKREITQVVRGPRLKQPHESLSEP
                     LFNGPSRERRERRKVRYDREARLKEGETSLAALTRQIEQAERDLQWVEKFEE"
BASE COUNT         1373 a         1665 c         1641 g         1527 t
ORIGIN      
        1 cagcccggcg ggacttgaaa cgctcgatca atttttctgc atcttgcacc tgagttttat
       61 gctgcagaat ggcttgcaat ttaatatccc agaattcggt cacatctatg gtggtgttgg
      121 gctggttggt cagcgagcac caaacttcct tgggcatatg tggctcgaaa ccttcatcca
      181 taagttcggg gaagtacgcc acatttccag ccgcagggaa gactgcatcc aacacgatct
      241 gcccgcaggc gcggtgatcg gggtggttga tgccatataa cgcaaaaaga ttttgcggat
      301 cgcaggtgag cagaatgtcc ggtttgtgcc tgcggatctc gcgcaccacg tctcggcgta
      361 gattcaggtc tgggacgagg tagccatctg gcaggtccat gaaaagaggc ggttcggctc
      421 cgatgacctt ggcggcttca gcttgttcaa tgtgtcgaag tccgcacagt ttttcaggag
      481 tcatatctgg cgtggtggtg gggttgaagc ccttatcgcc acaagttagt agcaagtatt
      541 gaatgctgtg accttcgcgt gcccacttgg caagcgtggc gccacaaaaa aattctggat
      601 catcggggtg agccagaatg accagaattt tttgttgagt ttcccaggta ttagtttgct
      661 cgggcatttt tgttgttctt tgctttgccg caggtgcagc cgccgccttc atggcggtcg
      721 cagggggctt cagattttcg gcgcagggct tcgagttcat cccacggttc gatctcttca
      781 cgcaggaagg tgtgatgctg cgggcgctcg ttggggattt cgatcagcac catgacctta
      841 tcgctcatgg gcatcacgtc aatcactttg ccttcgcctt tgggcgtgac cacgcgcttg
      901 ttacgcttgg gcaacgtctt gcgggcttct acatattgtt catactcata aatgagacag
      961 cagcgtaagc gcccgcacat gccggtgatt tcatcgggag ttagtgagat gccttgttcc
     1021 ttcgccatct tgatcgagat gggggagaaa tcggttagga acttggagca gcaacgggtt
     1081 tcaatgccgc acgcgcccat gccgccgatg agttttgcca catcacgcgg accgatctga
     1141 cgcatttcaa tatgcgtgtt tggatagatg tcgctcatat ccttgcgcag cgatttgagg
     1201 tccacctttt cttcttgttc ggtgctgaac aagaaggcga ggcgtgaacc gtcgtagttg
     1261 tattcagcgg caaccacttt gatggtctcc agcaagccca gctctgaagc gcgggcgcgg
     1321 caattgatca tggcttcggt ctgtttgttg cgccatgatt gttggaggag caggtcgcgc
     1381 ggcgtggcgc ggcgctctac agatttccag ccgccttccg gtttgggagg agcttcctga
     1441 agaatctgca caacctcgcc aaggtggcgt ccgcgcgtgg tatcgacgat gacatgttcg
     1501 ccttgtttga ggtctggcag ggaggatgaa tcgaagtgat aaatcttgcc gatgggggta
     1561 aaccgtacac cgataatgtt tttaacgtct gtttgcatgc gcgtaattat acctgagacg
     1621 ttcaggcaga gggagttttg aggaaataaa gagaggtcac agaaacctct gtgacctctg
     1681 cctgtgtatg gctgctttac ccgaacgaaa tgcccagatc gcgtgccgtg agcacaatat
     1741 cagactccat cggcaccatc ttcatctgac cgatcgcttc ggcaatcggc acatacttga
     1801 cgtgcggcgg gtcaagcgcc accatcacac cagcttgccc ctcttccaat ccgcgcacgg
     1861 cggcggcacc aaagcgtaac gcggtgaggc ggtcgaacga ggtgggcgag ccgcccctca
     1921 aaaggtgacc cagcaccacc acacgcgtct ctttgcccga aatggcatgg atctctgccg
     1981 ctactttttc gcccaccccg cccaggcgtt cggcacgacc cgcttcatgc tccactacgg
     2041 aaacgccgcc acccttgggg ctggcgcctt ctgctaccac cacgatcgta aacttattgc
     2101 cgctttcttc acgttcacgg attttttcca ccactttgtg aatatcgtag gggtgttcag
     2161 gaatgagaat gacgtcggca gaaccagcca cgccggcatg cagggcgatc caaccggcat
     2221 agcgacccat cacttctact accatcacac gtccgtgcga ggcggcagta gagtggagtc
     2281 tgtcgagcgc ttcggtggca aagcccacag cggtatcaaa cccaaaggtg acatcggtct
     2341 tatcaaggtc gttgtcgatg gtcttgggaa cggaaataag ccgcagccct ttttgagcaa
     2401 gcacgttagc gattgccaac gaaccatcac cgccaatggt gatgagcgca tcgatgtgat
     2461 gcaggcggaa gccgcgcaca atttcatcgg tgcggtccac ttcttcaata gtgccatcgg
     2521 ggcgctggat cggatatttc atggggttat tacgattgct ggtgccgagg atcgtgccgc
     2581 cgaggtgggt gatgccgcgc acacgttgac gggtgagagg catgaacccg ccatctggat
     2641 attcttcggg ggaaagaatg ccgtggaaac cgtccaagac tccatacact tcccagccac
     2701 gtttaatggc agagagagcg gcggcgcgaa tgaccgcatt caaaccgggg gcgtcaccgc
     2761 cgccggtgct gatcgcaatt ttcttaatag ttgctgacat gggaaactcc tgcgataagg
     2821 gcattatcta ttgataaacg ttcttatcca acatgtggga ttttttaaaa gtcattatag
     2881 ccctcaagcc tgccagatgg acatactccc ccagaagtat agacagaaat catgccaagg
     2941 aggcgaagca agtcataaaa attatcattc aagagcaata tttcacaggc aaagggctgg
     3001 gtatactgac acgcatgaac aaagtcgtat ccatcgacaa gaccgccctc ttacgctccg
     3061 ccttcaacgg cttatcggag aacgagctgg aagaaatggc ggcgctgacc gaagtgcgta
     3121 tctacccgcc tgagcatatc ttatgccgtg aaggggaata cgaagaaacc ttctacatca
     3181 tcgccgaagg aacagcgctt atcagcaaat acattggtga ccaggaaggt gaccgtgttt
     3241 tgcgcacggt tggcaaagga gacttagtgg gcgagatggc gttgatccag aatgcgccgc
     3301 gttctgccac cgtgcgtgcc acctctgaac tcatcgccct cgaaatggac aaagaccact
     3361 tcgaaaccat gttggggcgc agtccgcaca tggcgttgga tatcatccgc atcaccctca
     3421 accgcttgcg cgagaacgac cagatgatga tctctgaatt gcagaaatcc aacaaggtct
     3481 tacaacagct tgaccggaat aaaactgaat tcatcgacgt ggtggcgcat gaattgcgca
     3541 caccgctgac cgttttgaag ggctattcca atttactaag cgcatcgcct gaggtaaaaa
     3601 atcaccccac cctgcgcgat gtctctgaag gcatccaaag cggagccgac cgcattcacc
     3661 tgatcgtcaa caccatcttg gatgtctcgc gcatcgacaa caacgagttg accttacgcc
     3721 gttccccgat tctgctcaag caagtcgttc tagaagtcct tagcggatta accaaagccg
     3781 cctccgaacg gaccattgaa ctgttccacc ttcccggagc agatacgcct ctgattcatg
     3841 gcgacccggc tctagttcat aaagccttat atcatctcgt catcaatgcc atcaaataca
     3901 cccccaacgg tggtaaagtg accatcgcca cccgtccatc gaaactcgac aatggcgagg
     3961 aaggcgtttt gatcagcgtc caagataccg gcattggctt ggaccctgaa catcatgaac
     4021 tggtcttcga aaagttctat caagccgggc atgccgccat ccactcttcg ggcagcacca
     4081 gcttcaaagg cggcggaccg ggcttgggct tagccattgt gcgcggcgtg gcacgcatcc
     4141 acggcggcaa ggcttgggtg gaaagcacag gtgaagacga agtgaacttc cccggcagta
     4201 ccttctattt gtggttgcct gtatagagac ctaaaataaa agaggacgga ttttttatcc
     4261 gtcctctttt tatatcctta cagcggcttt cgatagatca cattgcgatg gatctctttg
     4321 taccccaagc tttggtacaa gcccaatgcc ccgctcgggt tttcagcatc cacaccaagg
     4381 gcggtttcgc tcatgccttg gtctttgaac atggcgatac tttttacaag caatgccttt
     4441 gccagcccgc gtttgcgcca cggtctgcgg accgagatgc cttcggtata cccacgcttg
     4501 cgatggtatt cacggttctc agcgttgctc acaaagttca agacctgccc ggcgatctgg
     4561 tcgccatccc aagccaccat ccatagaccg gggttgaaat cttcttcctc tgcccaatgc
     4621 atgaactctt caaaggtctg ttcgttgaag ccccaatgat cgcggaaggc ttcggtatgg
     4681 gcttcataaa cggcacgata gtctacctct gtcaccgggc gtacttccaa gccagatggc
     4741 agggaaactt cgggcaaggg ttcatggata tcacgtgcca tctcaaagaa ataacgcgtg
     4801 gactgatagc cttgcttttc gaggagggcg atcaagccct gagcggtgcc atttgcccac
     4861 atggagaaga ctttggtttc gggtccggtg tgagccgcgg cgatctggcg caaacgctct
     4921 tcggcatgtt ggaacatggc ggtgccgatc cctttgcgac gccatgcagg cagcagaaaa
     4981 ccgatgccgc tataggtgcg tgtcacgccg tcgttaagtt gatcccacca gacgcgctga
     5041 tagccgatca cctcgccgtt gatttctgcg aagagcatat cggtatacgg gtcacagttg
     5101 ttgaggtggc tgtaattgcg ccgtacatcc tcaaggctgt cggtgcgctc cacctggtcg
     5161 acctgcttgc tgccttcaat gatcgccagc atttttgggt aatcactttc accggcaaac
     5221 ccacggaagg aaagccctgc aatggcaggc gcatgcttta cttggatgtt cttcatggta
     5281 gacatgtttt tattatatcc ttgtaacagg gcagtcgatt catttgctag gatgacgact
     5341 tgttaaatca aaagagacca gagaagttat ttccatctct ggtctcttgc tgttcattaa
     5401 ctattcttcg aacttttcaa cccattgaag gtcgcgttcg gcttgttcga tctgacgcgt
     5461 cagcgcggcg agactggttt cgccttcctt caagcgggct tcgcggtcgt agcgcacttt
     5521 gcggcgttca cgtcgttcgc ggctgggacc gttaaacagc ggctccgaaa gggactcgtg
     5581 cggctgcttc aaacgcggac cgcgcaccac ttgggtgatc tcgcgttttt cttcctgcag
     5641 cctgcgtttg cgcatcttca attccgtcaa caaagcatgc ttttcgtcca acgagcgcgg
     5701 cgtgaaatcc atcatctctt gcaattgctg gatggctttg ctcagcccat tcttcgaagt
     5761 cttcagttct tcgatttgct gtgccagctc tttttcttcc ttgccaacac cgagcagacc
     5821 gcccagcacc tttttggagc gttcatattg cagtttctca agcgcctgcg tatgccggaa
     5881 gacctcttca tctatttcgc gggcgttttg gttaagttgc ccgatctcat aaatagctcg
     5941 ttcgctccat tccttcgctt cggcaaaggt ggagagcgag agcattccgg ttaataattc
     6001 ttgcagacct tcgtcgtaaa gagcatggac agcattcata cgtttaattt cctgggttta
     6061 acttgttgaa gtgatttcaa gaattctaaa tgaatcaacc cgttggaaat caaaacttca
     6121 cgtaggaatg gctaacagtt ttgttggttt cttactggca ggcgctggtc tcggcttgca
     6181 ccttggtggg cgattcgccg ccggtg
//
LOCUS       DAOREF010000035         6202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6657_length_6202_cov_4.065723, whole genome shotgun sequence.
ACCESSION   DAOREF010000035 DAOREF010000000
VERSION     DAOREF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_6202_cov_4.065723"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKI33_01255"
     CDS             complement(<1..1144)
                     /locus_tag="PKI33_01255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNM35653.1"
                     /translation="MSEFPTQAQVVIIGGGVGGSSIAYHLTKLGWKDVVVLERHELTS
                     GSTWHSAGLVGQMRSDANLTRMMHYSTDLYRSLKAETGQDTSWREVGGIRLASSHERM
                     EETKRLVGLARSFGVPMELISPKEAQDMFPLMDITGVVGAAYTPNDGSIDPTGLTNAL
                     AIGAKNRGARFFFYTNVEKINVVNGRVSEVVTDKGTIKTEIVVNASGMWGREVGKMVG
                     LNLPVIPMAHLYILTEPIKGVTNKFPNLRDPDLLVYWREEVGGLVTGGYERQPATFGM
                     SGIPRDFKFKLLEPDWERFTPLMENSIRRVPAVESAPVKLLLNGPEGFTPDGEFLLGP
                     TSVKGFWVACAFCAHGLAGAGGMGKVMAEWIVDGSPEWDVWRLDVRR"
     gene            complement(1243..2052)
                     /locus_tag="PKI33_01260"
     CDS             complement(1243..2052)
                     /locus_tag="PKI33_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNM35654.1"
                     /translation="MTKLVKDLMHKGVITCKPDATLGQVATLLDQHQVHALFVTDRDG
                     RIVGVISDFDLMTGEWLSSDPESLNTMRTLTASDLMTKPVDTIEAATPLTEAADLFMQ
                     KKVGRFLVLEHEKPVGTISLSDFVASLSERIKSKREKVGDVMSHAILVCRGKTPVLSA
                     AYAMTSAGWRSVLVVDLNGKVIGVVSGHDLLHLVKNGVDEKLTVRDVMHPALTIDINA
                     SLREAADMLIQNHYHRLVVVDKENPDSFPLGAISTFDIVAEMARPDSVWQK"
     gene            2180..2968
                     /locus_tag="PKI33_01265"
     CDS             2180..2968
                     /locus_tag="PKI33_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNM35655.1"
                     /translation="MGKPLIPAQRREKIQEYLGIHQIARTADLMDLLEASEATVRRDL
                     EWLEQKGILERTHGGAVLNQRVIFEQEYQLRLKNYPDEKKQIGEFAATLIEEGDIVFI
                     NSGTTATQVLQHVPRNPRITVFTNNVSALVDIGDPGFSLNLTGGEFQARSNSLSGRFA
                     LDNLNQVFANKAILGVDGISLKHGLTVPTNPEAEVVRKMIERTKGQVIIVADHSKWGA
                     VSNFHVASIEAMDKLVTDTGFSKQAKKELADYSIEVFLANNMKV"
     gene            3052..5481
                     /locus_tag="PKI33_01270"
     CDS             3052..5481
                     /locus_tag="PKI33_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM35656.1"
                     /translation="MKTHAEIVVIGGGIYGAQVAYHLAKNGRKDVVIVEKGEVASGES
                     SHAAGLVTQFATSQAMLRFRMYSVQLYKELGLFDTVGSLRVASSKEQLLEMERSVSRA
                     KALGLDCEVISPEESKKYMPQISDQDLYGGIYLPGDGQLDPYTVTTSMARFAKELGVE
                     IYTNTRVTGIKVSAKGEVEAVVTDKGTIRCEIIVNAAGMWAPRIAAMAGLHVPTTPVD
                     HQHIALRAVPGHEFNDQTPCLRDPDNLVYMRQEKGGLVIGGYEPKPLPRWIDGTPWEH
                     GSKSFPGDFDQFEMLLEGAIRRLPFLDQAGIITLVRHPGAYTPDCQPLLGPMPGVKGF
                     WMMAGMSLNGYGGAGGMGKLMAEWIMDGEAPMDVYGYRATRFGNYYSDFNYAAERTVE
                     AVKYYYRLKFPHDESEAARPHRTSPVHYRLMENGAVFGEKFGWERVNYFNPGNEWRRM
                     GEDQREWGWTKPPYFERMRVEHIATRERVALFDLTSFGKIEVKGPGALPLLQRLTSSN
                     IDKPVGSAVYTQFLNRNGGIESDLTVTRLGEAYFWVITGSGFIANDHARILMNVNEAD
                     GEVSIRDITQEHACLALWGPKARDVLKKITSSDVSNEGHPYLTTKPIQINGVKVIAQR
                     VSYAGELGWELYIPNNRATMVWDMLVEAGKEFGMELGGYKVLDPLRLEKGFKYFTTDI
                     TPTTTPYEAGLGFCVDLNKGDFIGRDALVKQKADGVTRKLCTLVLTDTDEFVQIYGGE
                     AVHHEGKVVTRIRSGGYGFTVKKNILYAYLPVDLAVKGTRFTIELIEGNLAAEVTANV
                     LYDPKSEKLKA"
     gene            5500..>6202
                     /locus_tag="PKI33_01275"
     CDS             5500..>6202
                     /locus_tag="PKI33_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNM35657.1"
                     /translation="MAKKNSSPAYIGFGMLTPVYIMSLDQLPKLNTGAIVHQVSDYVY
                     DDAAIIACNLSQWNVPSAMIGTAVGKDLLGKYVADKLKELGVKGEVRFTTKYKTPLEV
                     NVSDKKGARTYFWQRSKEILDTLSTADLSLIKSAKLLYVDWYDGEHIVRAMAEAKKHN
                     VPVFLNFEHGHQNPDLLKKYAGMVTVCQAVTDAAQIGKKQAMLGTARKLIKSGIQTAI
                     ITMASQGCMVVQGDEI"
BASE COUNT         1562 a         1416 c         1674 g         1550 t
ORIGIN      
        1 agcgacgcac atccagacgc cagacatccc attcggggct gccatctacg atccactctg
       61 ccattacctt gcccataccg cccgcgcctg cgagaccatg cgcacagaag gcacacgcca
      121 cccagaagcc tttgacggat gtaggaccca acaagaattc gccatcgggt gtgaagcctt
      181 cgggtccgtt gagcagcaat tttacggggg cactttccac cgcaggcaca cgccggatgg
      241 agttttccat cagcggagtg aagcgctccc aatcgggttc gagcagtttg aatttgaaat
      301 cacgtggaat gccgctcatc ccaaaggtgg cgggttgacg ttcataccca ccagtgacaa
      361 gcccgcccac ttcttcgcgc cagtacacga gcaggtctgg gtcgcgcaga ttggggaatt
      421 tattggtcac gcctttgatg ggctcggtga ggatgtacaa atgcgccatg gggatgactg
      481 gcagattcaa gccgaccatt tttcccactt cgcgtcccca catgccggag gcgttgacca
      541 cgatctcagt cttgatcgta cccttatccg tcactacttc gctgacgcgc ccattgacga
      601 cgttgatttt ttcaacgttg gtatagaaga agaaacgcgc gccgcgattc ttggcgccaa
      661 tggcgagagc gttggtaaga ccagtcgggt cgatggaacc gtcgttagga gtgtaagctg
      721 cgccgaccac gccggtgatg tccatgagcg ggaacatgtc ttgcgcttct ttgggagaaa
      781 tcaactccat ggggacgccg aaggagcggg ctaatccaac gaggcgcttg gtctcttcca
      841 tacgttcgtg tgatgaagca agacgaatcc cgccgacttc gcgccatgac gtgtcttgcc
      901 cagtttcggc tttgaggctg cgatataggt cggtgctgta gtgcatcatg cgggtcaggt
      961 ttgcgtcaga gcgcatctgc ccgactagcc ctgcagagtg ccaggtggag ccggaggtca
     1021 actcgtggcg ttcaaggacg acgacatcct tccaaccgag cttggtgagg tgataggcga
     1081 tactgcttcc acccacgccg ccgccgatga tgacgacctg ggcttgtgtt gggaattcag
     1141 acatgaaagt ctcctaagag cgtttaccac gaaggacact aaggtcacga agttttttta
     1201 tttttccctt tgtgtacttc gtgccctttg tggttaagat ttttactttt gccaaacaga
     1261 atcgggtctt gccatttctg ctacaatatc gaacgttgag atcgcaccaa gtggaaacga
     1321 gtcagggttt tctttatcga ccacgactag tcggtgataa tgattttgaa tgagcatatc
     1381 tgcggcttcg cgcaggctgg cgttgatgtc gatggtgaga gcggggtgca tcacatcacg
     1441 gacggttagt ttttcatcca cgccgttctt gacgagatgc aagaggtcat gtccgctcac
     1501 tacaccgatg actttgccgt tcaagtccac aaccagcacc gagcgccagc cggcagatgt
     1561 catggcatag gcggcagaaa gaactggagt cttgccacgg caaacaagaa tggcatgcga
     1621 catcacatcg ccgactttct cgcgtttaga tttgatccgc tcggaaaggc tggcaacaaa
     1681 atctgaaagc gagatcgtgc caactggttt ttcatgctct aaaactaaaa accgcccaac
     1741 ctttttttgc atgaaaaggt cggcggcttc cgtcagagga gtagcggctt cgatggtgtc
     1801 aacaggcttg gtcatcaggt ctgaggcggt gagtgtccgc atggtgttga ggctttcagg
     1861 gtctgaagaa agccattcgc ctgtcatcaa atcaaagtct gaaatgactc ccacaatccg
     1921 accatcgcga tctgtgacga acaaggcatg cacttgatgt tgatcgagca aggtagctac
     1981 ttgacccagg gttgcatcgg gtttacaagt gatcacgcct ttgtgcataa ggtctttgac
     2041 aagttttgtc atgaagtctc caaaaaagaa gacccgacag gttttgtgag attaagctgt
     2101 caggtctgtg ttacaaatcc attataagca aaaaatcaaa aacttgcaaa ttccttcaga
     2161 ttcttatata atccgtcaga tgggtaaacc cctcattcca gcccaacgta gagaaaagat
     2221 tcaagagtat cttggcatcc atcaaatcgc gcgtactgcc gacctgatgg atttgcttga
     2281 agcttctgaa gcaaccgttc gccgcgactt ggaatggttg gaacaaaaag gaatcctaga
     2341 gcgcacgcat gggggcgcgg tcttaaatca acgtgtgatc ttcgaacagg agtatcaact
     2401 ccgtctcaag aattatcccg acgaaaagaa acaaatcggt gagtttgccg ccacccttat
     2461 tgaagagggt gatattgttt ttatcaacag cggtaccact gctacacaag tattgcagca
     2521 cgttccgagg aatccacgca ttacggtttt tacgaataac gtcagtgcct tggtggatat
     2581 cggtgatccg ggcttttctc tcaatttgac cggaggcgaa ttccaggctc gttccaactc
     2641 tctttccggt cgcttcgcgc tggacaattt gaatcaagtc ttcgctaata aagccattct
     2701 cggcgtagac ggaatctccc taaaacacgg gctgacggtt cccaccaacc ctgaagcaga
     2761 agtcgtccgc aagatgatcg agcgcacaaa gggacaggtc attattgtgg cagaccatag
     2821 caaatggggt gcggtttcta attttcatgt agcaagtatc gaagcgatgg ataaacttgt
     2881 taccgacact ggttttagta aacaagcgaa aaaggaactg gcagattatt cgatcgaagt
     2941 ttttcttgca aataacatga aggtctaaca caaatataaa acacgtttta agacatttgt
     3001 tttgaacgtg ttttcttgtt ttacatcaca atcaaactac ggagttgatg aatgaagact
     3061 catgcagaga tcgttgttat tggtggggga atttatgggg cacaggttgc ttatcacctt
     3121 gccaagaacg gacgcaagga tgtcgtcata gtcgaaaaag gggaggttgc tagcggtgag
     3181 tcttcgcatg cggcggggtt ggtgacgcag tttgccacct cacaggcaat gttacgcttc
     3241 cgtatgtata gcgtgcagtt atataaagaa cttggtttat tcgatacagt tggtagtttg
     3301 cgcgtggctt caagcaaaga acaattgttg gaaatggaac gcagcgtgag ccgtgccaag
     3361 gcgttgggac tggattgtga agttatctca ccagaagaat ccaaaaaata tatgccgcaa
     3421 atttcggacc aagatctgta cggcggcatt tacctacctg gtgacggtca actcgatccg
     3481 tatactgtca ctacatcgat ggcgcgtttt gctaaagaat taggcgtcga gatttatacc
     3541 aacacccgcg tgaccggaat caaagtgtca gcgaagggtg aggttgaggc ggttgtaaca
     3601 gataagggca cgattcgatg cgaaatcatt gtcaatgccg caggaatgtg ggcgccgcgc
     3661 atcgctgcta tggcgggtct gcatgtgcca accactccgg ttgaccatca acatattgcg
     3721 ttgcgagctg tgcctgggca tgagttcaac gatcagacgc cttgcttgcg tgacccggat
     3781 aatttggtgt atatgcgtca ggaaaaaggg gggcttgtga tcggtgggta tgagccgaag
     3841 ccgcttccac gttggatcga tggtacgccg tgggaacatg gcagtaaatc tttccccggt
     3901 gatttcgacc aatttgaaat gttgcttgaa ggtgcgattc gtcgtttgcc gtttttggat
     3961 caggcgggca tcattacgtt agtacgtcac cccggtgcgt acacgcccga ttgccagccc
     4021 ttgctcggac cgatgcccgg tgtgaaaggt ttctggatga tggcgggcat gtcgctcaat
     4081 ggctatggtg gcgcaggtgg catgggcaaa ctgatggcgg aatggatcat ggatggtgaa
     4141 gcaccgatgg atgtgtatgg ctatcgtgcc acacgctttg gcaattacta ttctgatttc
     4201 aactatgccg ccgaacgaac ggtggaagcc gtaaagtatt actatcgttt gaaattcccg
     4261 cacgatgaat cagaagcggc acgtccgcat cgcaccagcc cggtgcatta tcgtttgatg
     4321 gaaaatggcg cggtttttgg cgagaagttt ggctgggagc gcgtgaatta tttcaaccca
     4381 ggtaatgagt ggcgtcgtat gggcgaagat caacgcgagt gggggtggac gaaacctcca
     4441 tacttcgaac ggatgcgagt ggaacatatt gcaacccgtg agcgtgtggc actgtttgac
     4501 ctaacctctt ttggcaagat cgaagtcaaa ggacccggtg cattgccttt gttgcaacgc
     4561 ttaacgtcga gtaatattga taagcccgtt ggcagcgcgg tgtataccca attcttgaat
     4621 cgcaatggcg gtattgagtc tgatttgacg gtcactcgtt tgggtgaagc atatttctgg
     4681 gttattacag gttctggttt tattgccaac gaccatgcgc ggattttgat gaacgtgaat
     4741 gaagcagacg gcgaagtgtc tatccgtgat attacgcagg aacatgcctg tcttgccttg
     4801 tggggaccca aggcgcgtga tgtattaaag aagattacta gcagtgacgt gtctaatgaa
     4861 gggcatcctt atctgaccac aaagccgatt caaattaacg gcgtgaaagt gattgcacaa
     4921 cgagtaagct atgctggcga attgggctgg gagttgtata ttccgaacaa tcgcgccacc
     4981 atggtatggg atatgcttgt tgaagctggt aaagaatttg gcatggaact cggcggctat
     5041 aaagtgctgg acccactgcg tttggaaaaa ggctttaagt atttcacgac cgatattacc
     5101 ccaacaacca caccctatga agctggtctt ggcttttgcg tggacttgaa caagggtgac
     5161 ttcatcgggc gcgatgcgtt ggtcaagcaa aaagcagatg gagtaacgcg gaaactctgt
     5221 actttggtat tgaccgatac cgatgaattt gtgcagatct acggcggtga agcggttcat
     5281 cacgaaggga aagttgtcac tcgtatccgc agtggtggct acggatttac cgtgaaaaag
     5341 aatattttgt atgcctacct gccagtggat ttggcagtta aaggcactcg gttcaccatt
     5401 gaattgatcg agggcaatct cgctgctgaa gtgactgcca atgtcttgta cgacccgaag
     5461 agtgagaagc tgaaagcgta aattgattac cctctaatta tggctaagaa aaattcctct
     5521 ccagcttaca ttggctttgg catgttgacg cctgtgtaca ttatgtcgct ggatcaattg
     5581 cccaagttaa atacaggtgc catcgtgcat caggtcagcg attatgtgta tgacgatgct
     5641 gccatcattg cttgcaacct tagccagtgg aatgtaccgt ctgccatgat cggaacagcg
     5701 gtggggaaag acttgcttgg aaaatacgtc gcagataaat tgaaagaatt gggcgtaaaa
     5761 ggggaagtgc gctttacgac gaaatataaa acgcctttgg aagtgaacgt ctctgacaaa
     5821 aaaggcgcgc gcacgtattt ttggcaacgt tcaaaggaaa ttcttgatac attgtctaca
     5881 gcggatttgt cactgatcaa atctgcaaaa ctattgtatg tggactggta cgatggcgag
     5941 catatcgtcc gtgccatggc agaagccaag aaacataatg tgcccgtctt tttgaacttt
     6001 gagcatgggc atcagaaccc agacctgctc aaaaaatatg cgggcatggt cacggtctgt
     6061 caggcagtga ccgatgcggc gcagatcggt aagaaacaag ccatgttggg cacagcacgc
     6121 aagctcatta aatctggcat tcaaacggct atcatcacta tggcaagtca ggggtgtatg
     6181 gtggtgcaag gtgatgaaat ta
//
LOCUS       DAOREF010000036         6158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6727_length_6158_cov_4.183844, whole genome shotgun sequence.
ACCESSION   DAOREF010000036 DAOREF010000000
VERSION     DAOREF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6727_length_6158_cov_4.183844"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PKI33_01280"
     CDS             <1..1284
                     /locus_tag="PKI33_01280"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNM35658.1"
                     /translation="ESSAKFHEKWVVGYGHASVAEHAVLHIAFENVSRVAIETIEGNR
                     LASYTEKSTRYQKWGSDDFTIPPELDGHPLRDEFIATVRLLFSTYAESLDPLKNLIVE
                     RTPRRENEADEAYDRRIRSQYVDRCRFLLPAAANANVGMTANARVIEMTIRKMLSHPL
                     EEVRQIGEKLKEVSKGETPTLVKYADANPYTVETIRDLENRPSQIENRKSDWCELIEY
                     DKDGEKKILAAALYRFDEIPYADALAYVKALKKKEREALAETLLGKLGKYDTPLRELE
                     YSNYTFDLVMDQGAYAEFKRHRMMSQTPQRLTTRLGYTTPLLITEAGFGSKYEAAMEA
                     ASQMYEKLHAFNPDVAQYIVPNGFNRRVLAQFNLREAFAFCQLRTAANAHFSIRRVAQ
                     KIYEDISKVHPLLSKYMKLREETWQSVEENYFSKI"
     gene            complement(1325..2311)
                     /locus_tag="PKI33_01285"
     CDS             complement(1325..2311)
                     /locus_tag="PKI33_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNM35659.1"
                     /translation="MQYRHLGRTGIQVSELSIGSWVTFKNQVDVQAAVEIMAAAYDHG
                     VNFYDNAEVYAGGKSEEVMGAALKKLNWRRGSYLVSTKFYWGIEEGVNEKNTLNRKRL
                     IEGINGALKRLDMDYVDLIYCHRPDKTTPIEETVWAMHNIIEWGKALYWGTSEWAASE
                     IIEAIQIAERHHLHKPVVEQPQYNLFERKRLEGDYVRFYKEYGYGTTIWSPLASGLLS
                     GKYNKGIPADSRGALKGYEWLHGSLTNQEKLAKVAGLEPIAKELGGTVAQLAIAWCLK
                     NPFISTVITGASRVEQVHENMKSAEIAAKLTPEIMGKIDVVFGVQKKDEDDD"
     gene            2374..3285
                     /locus_tag="PKI33_01290"
     CDS             2374..3285
                     /locus_tag="PKI33_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM35660.1"
                     /translation="MRKTLLLLSFVIISACAPAPAQTTDVSLQAYLTATPIATSTPNV
                     LVVVETPMPTSTLQVYTVQSGDTISQIAEKFKIPQENLIAANPDVNPNSLTIGQTLFI
                     PDPSAPLAAASTPTPLPVPISQAACHPTADSGNWCFALIQNNTNSILENVFARITLLD
                     KSGNALVYQTAYLPLDVIPANASLPVYVYFPNTPADVQPQVQLLSAMTGGSAYLPISL
                     DNTSAQMDWNGKTAKVSGQAYLPAESNAATQVWIAAVAFDKYGTVVGVRRWEGGALQP
                     GSSLNFDFSVSSVGGEIEAVEFFAQAK"
     gene            complement(3337..4788)
                     /locus_tag="PKI33_01295"
     CDS             complement(3337..4788)
                     /locus_tag="PKI33_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNM35661.1"
                     /translation="MRDYDPATTYSAGDKLIFSGLVQLDQNLNIRPDLAETWEVTGDG
                     TIYTFKIRNNAKFHNGRDVTAQDVVFSWERAASPELASDTVLTYLSDIVGVREKALGQ
                     ADSISGLKVIDDKTLQVTIDAPKPYFLMKLTFPTSYIVDQDNVSAGEEWVRQPNGTGP
                     YKLKEWRSNEYIVYEANPDFYLGAPSIPYVVFKLYAGDDVRLFETGDVDIAGVGLYDV
                     ERMLNPNEPLNRNLVTGVDLCTSYVTFDTTQPPFDDANVRKAFSMAFDRQRYVDVLYR
                     GVALPAEGLYPPGLPGFDYNLQGLPFDPEQARELLKQSKYGGPEGLPEIIYTNAGIGS
                     YIGGNVAALAEMWQDYLGVKIKVENLDYNFYYQQIFSGNHGQIFDGGWCADYPDPENF
                     ADVLFHTGVPQNQGGYSNPELDTLLEAARIEPDVTKRIAMYQEAERIIVNDAPVIFTT
                     HSLSYQLVQSYVKGYVNTPISISAERFMWLEGK"
     gene            complement(4792..4953)
                     /locus_tag="PKI33_01300"
     CDS             complement(4792..4953)
                     /locus_tag="PKI33_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35662.1"
                     /translation="MDKFKTIHRRFTVGGLALVLLASLFLTASAPSDPDFQIGVPLDQ
                     AIVYSGGES"
     gene            complement(4946..>6158)
                     /locus_tag="PKI33_01305"
     CDS             complement(4946..>6158)
                     /locus_tag="PKI33_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNM35663.1"
                     /translation="SAEITAVTLEYGSEQLNCGEVVAKAFPDFSPSPSIDVSWTWDMR
                     QSGSLPPGTTIWWRWRYVDSAGKEYLSDTNTVIWLDNDHDWQTATGTNIRIHYYGASQ
                     SFAQSMVDAGLEGLRRNKDQAGMTPDIPVDIYVYPSYDEMRDAILYEPQWTGGLAYSN
                     FSIIIMGTSGDANFDKATVIHEVTHVLVGRNAFTCIGFIPTWLNEGLAVYNEGPLDES
                     MKAQFDIALRDDTFFPVRSLSGNFSEIPEKALLSYAQSHSVVKFMLETYGQEKMSQLL
                     QALSDAEPVDDALVQIYGVDTDGLDAAWRESLGLQPLVVAQATSQPTPTYVPTYVPIA
                     GIPQAITATPGAIPTSSSTNNSEPSFSGPPIVLTVVMLCICLVFLTIIGVVVLGIVLR
                     NNKGNGGKKNG"
BASE COUNT         1633 a         1576 c         1413 g         1536 t
ORIGIN      
        1 gaatcttcag caaaattcca tgagaaatgg gtcgttggtt atggtcatgc ctctgtagcc
       61 gaacatgctg tcctgcatat cgcgtttgaa aatgtctcac gtgttgccat tgaaacaatc
      121 gaaggcaatc gccttgcttc ttatacggaa aaatccactc gctaccaaaa atggggctcc
      181 gatgacttca ctattccgcc agaattagac ggacatcccc tgcgcgatga attcatagca
      241 accgtccgcc tccttttctc gacctatgct gagtcattgg acccgctcaa gaatctcatc
      301 gttgagcgga ctccccgccg tgaaaacgaa gcggatgaag cctatgatcg ccgtatccgc
      361 tcacaatatg tggaccgttg ccgctttctg ctgcctgccg ccgccaatgc caatgtaggt
      421 atgaccgcca atgcgcgcgt catcgaaatg accattcgca aaatgctttc tcacccactc
      481 gaagaagtac gtcaaattgg cgagaagtta aaagaagtct ctaaaggcga aacacccacc
      541 ttagtcaaat atgcagacgc taatccatac acagtcgaaa ccattcgtga tttggaaaat
      601 cgtccatctc aaatcgaaaa tcggaaatca gattggtgcg aacttattga gtacgacaaa
      661 gatggggaaa agaaaattct cgctgcggcg ttataccgct ttgatgaaat cccctatgcc
      721 gatgctctgg catacgtcaa agcgcttaag aaaaaagagc gtgaagcact cgctgaaacc
      781 ttgcttggca agctcggcaa atatgatacc cctctgcgtg aattggaata ttccaactac
      841 actttcgacc ttgtcatgga ccaaggcgca tatgccgagt tcaaacgtca ccgtatgatg
      901 agccagaccc cacaacggct gaccacaagg ctcgggtata cgactccgct gcttataacg
      961 gaagcaggtt tcgggtccaa gtatgaggcg gcgatggaag cggcatcaca gatgtatgaa
     1021 aagctccatg ccttcaatcc agatgttgcc caatacatcg tcccgaatgg atttaatcgc
     1081 cgggtgttgg cgcaattcaa tttacgggaa gccttcgcct tctgtcagtt gcgcacagct
     1141 gccaatgccc acttttcaat tcggcgcgtg gcgcaaaaaa tttatgaaga catttccaag
     1201 gttcacccac tgctttcaaa gtatatgaaa ttgcgcgaag agacttggca aagtgtggaa
     1261 gaaaattatt tttcgaagat ctaaacaaaa actccgaggt cttgaaacct cggagttttt
     1321 ttatttaatc gtcgtcttcg tctttctttt gcacaccaaa gaccacatcg atcttaccca
     1381 taatctcggg cgtgagtttg gcagcaatct ctgctgactt catgttttca tgaacttgtt
     1441 ccacacggct tgcaccggta atgaccgtgc taatgaaggg attcttcaag caccatgcaa
     1501 ttgccagttg cgccacggtg ccgccaagtt ctttggcaat aggctccaaa cctgcaacct
     1561 tcgccagttt ttcttgattg gtgaggctgc catgcaacca ttcataacct ttgagtgcgc
     1621 cgcggctatc agcagggatg cccttgttgt atttaccaga cagtaaacca gaagcgagcg
     1681 ggctccaaat ggtagtgcca tagccatatt ctttatagaa gcggacataa tcgccttcga
     1741 ggcgtttgcg ttcaaaaagg ttgtattgcg gctgttcaac taccggttta tgcaagtgat
     1801 ggcgttctgc aatttgaatg gcttcaataa tctcagaagc tgcccattca gaagtgcccc
     1861 aatacaaggc tttgccccat tcaataatat tatgcatcgc ccaaactgtt tcttcaatgg
     1921 gcgtggtctt atctgggcgg tgacagtaga tcaagtcaac atagtccatg tcgagacgtt
     1981 tgagtgcacc gttgatacct tcaatcagac gcttgcgatt gagggtattc ttttcgttaa
     2041 ccccttcttc aatgccccaa taaaacttgg tggaaaccaa ataactacca cggcgccagt
     2101 tcaacttctt caatgcagcg cccatcacct cttccgattt tccgcctgca tacacttcgg
     2161 cgttatcgta aaagttcaca ccatgatcgt atgccgcagc catgatctcc actgcggctt
     2221 gcacatccac ttgattcttg aaggtaaccc aagacccaat agataactca ctgacttgaa
     2281 tgcccgttct tccaagatga cgatattgca ttgttcctcc aaaagatttt atggcgaatc
     2341 cgccgtattt ttcgagcaca ttataatcac tgcatgcgaa aaaccttgct attgctttct
     2401 ttcgtaatca tatcagcttg cgcacccgcc cctgctcaaa ccacagacgt ctcacttcaa
     2461 gcgtacctta cggctacccc gattgccaca tctactccca atgtcttggt cgtcgtcgag
     2521 acacccatgc caaccagcac actacaagtc tataccgttc aatcaggcga tacgatcagc
     2581 caaatcgctg agaagtttaa gattccacaa gagaatttga ttgcggcaaa tccggatgtg
     2641 aacccgaaca gtctcaccat tgggcagacg ctcttcatcc cagacccatc tgcccctctc
     2701 gccgccgcct caacccctac gccgttacct gttcccattt ctcaagccgc ctgtcacccc
     2761 accgccgact cgggtaactg gtgctttgca ctcattcaaa acaatacaaa cagcattctg
     2821 gaaaatgtct ttgcccgaat cacattatta gataaaagcg gaaacgccct tgtctatcaa
     2881 accgcatatc tgcccttaga tgtgattcca gccaatgctt cgctacctgt gtatgtctat
     2941 tttccaaaca cacccgcaga cgtacagcca caagttcaat tgttgagcgc catgacaggt
     3001 ggttctgctt atttgcccat ctcgttggat aacacctccg cgcaaatgga ttggaatggc
     3061 aaaactgcca aggtcagcgg acaagcgtat ttacccgcag aatcaaacgc cgcaacccag
     3121 gtatggattg cggctgtggc attcgataaa tatggaacag tggttggtgt gcgccgctgg
     3181 gaaggcggag cgctccagcc ggggagttcg ctcaacttcg actttagcgt atccagcgtc
     3241 ggcggggaaa ttgaggcggt ggagttcttc gctcaagcaa aataaaatcc tttcctcttt
     3301 ctccttatcc aacaaaaaga aagaggaaag gaaaaattat ttaccttcca accacataaa
     3361 acgttctgcc gaaattgaaa taggtgtatt cacatatccc ttcacatacg attgcacgag
     3421 ttgatacgaa agcgaatggg tggtgaagat cacaggcgca tcgttgacaa tgatacgttc
     3481 ggcttcttga tacatggcaa tgcgtttggt cacatccggt tcgatgcgag cggcttcgag
     3541 taaggtatcc aactctgggt tggaatagcc accttgattt tgcggcacac cggtatggaa
     3601 gagaacatcg gcaaaatttt cagggtcggg gtaatcggca caccaaccac catcaaagat
     3661 ctgaccatga ttgccagaaa aaatctgttg atagtagaag ttgtaatcta aattctcgac
     3721 cttgattttc acgccaagat agtcttgcca catctcagcc aaagcggcta catttccacc
     3781 aatatagcta ccaatacccg cattggtgta aatgatctca ggcaagcctt cgggtccgcc
     3841 gtatttggat tgcttcaaca attcacgcgc ttgttcgggg tcaaagggta aaccttgcag
     3901 gttgtaatca aagccgggta aaccgggcgg gtataacccc tctgctggca atgccacacc
     3961 gcgatataac acatccacat agcgttgacg gtcgaatgcc atgctaaagg ctttgcgaac
     4021 attggcgtcg tcaaaaggcg gttgggtggt gtcaaaggtg acatagcttg tacaaaggtc
     4081 cacaccggtg acgagattgc ggttcaaagg ctcatttggg ttcagcatac gttctacatc
     4141 gtataagccg acgcccgcaa tatctacatc gcccgtttca aagaggcgca catcatcacc
     4201 tgcatagagt ttgaagacaa cataaggaat cgatggcgcg cccaggtaga aatctggatt
     4261 ggcttcgtaa acgatatatt cgttggagcg ccattctttg agtttataag gtcccgtgcc
     4321 attgggctga cgcacccatt cctcaccagc ggatacattg tcttgatcca caatatagga
     4381 tgtggggaag gtgagcttca tgagaaagta tggcttgggc gcatcaatcg tgacttgaag
     4441 ggttttgtcg tcgataactt ttagcccgct gatactatct gcctgaccaa gcgctttctc
     4501 gcgtactcca acgatatcgc tcaagtaggt gagaacggtg tcagacgcca actcagggct
     4561 ggcagcgcgc tcccaagaaa agaccacgtc ttgagccgta acatcccgtc cattatggaa
     4621 cttggcattg ttgcgaatct tgaaggtgta gatagtgcca tcgccagtca cttcccaagt
     4681 ttcggcaagg tctgggcgga tgttcaagtt ttggtcgagt tgcaccaaac cgctgaagat
     4741 gagtttatcg cccgcactat aggtcgtggc ggggtcgtaa tcacgcaaat tttaagattc
     4801 accaccactg taaacaatcg cctgatctaa cggaacacca atctgaaaat cgggatcgct
     4861 gggtgcgctg gcagtaagga agagagaagc aagtaaaaca agcgccaaac caccaacggt
     4921 aaagcgccga tgtatggttt tgaatttatc cattcttctt tcctccattg cctttattat
     4981 tgcgcagcac aattcctaaa accacaacac cgatgatcgt caaaaagacg agacagatac
     5041 atagcatcac aacggtcaac acaatgggcg gaccactaaa agaaggctcg gaattatttg
     5101 ttgacgatga agttggaatt gcgcccggtg tggcggtgat ggcttgaggt atgcccgcaa
     5161 taggaacata ggtcggcaca taggtcggcg tgggctgcga tgttgcttgt gccaccacca
     5221 gcggttgaag cccaagcgac tcacgccaag cggcatcgag cccatcggta tccacgccgt
     5281 aaatttgcac caaggcatca tcgacaggtt cggcatcgct aagcgcttga agcagttgac
     5341 tcatcttctc ttgtccatag gtctcaagca taaacttgac cacactatga gattgagcgt
     5401 atgaaagcaa ggctttttct gggatttcag agaaatttcc gctcaaggaa cggacaggga
     5461 aaaaggtgtc gtcacgcagg gcaatatcga attgcgcctt catgctttca tccagcggac
     5521 cttcgttata cacggcgaga ccttcattca accaagtagg aataaaccca atacaagtaa
     5581 atgcattgcg tcctactagc acatgggtca cttcatggat gacggtggct ttatcaaaat
     5641 tggcatcgcc agaagtgccc atgataatga tgctgaagtt tgagtatgcc aaaccgcctg
     5701 tccactgcgg ttcatagaga atggcatctc gcatttcatc gtaactgggg tagacgtaaa
     5761 tatcgacagg gatatctggc gtcataccgg cttggtcttt gtttcggcgt agaccttcca
     5821 aaccagcgtc caccatagac tgggcgaaac tttggcttgc accatagtaa tggatgcgaa
     5881 tatttgtgcc tgttgcagtt tgccagtcat ggtcattatc caaccagatg actgtattcg
     5941 tatcgcttag atattccttg ccagcagagt caacatagcg ccagcgccac cagatggtag
     6001 tgcctggcgg aagggatccg ctctggcgca tatcccaagt ccaggagaca tctatggatg
     6061 ggctggggga gaaatctggg aaggctttgg caaccacctc cccacagttc aattgctcgc
     6121 tgccatattc cagagtaacg gctgtaattt ctgcagaa
//
LOCUS       DAOREF010000037         6138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6766_length_6138_cov_4.079073, whole genome shotgun sequence.
ACCESSION   DAOREF010000037 DAOREF010000000
VERSION     DAOREF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6766_length_6138_cov_4.079073"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKI33_01310"
     CDS             <1..479
                     /locus_tag="PKI33_01310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNM35664.1"
                     /translation="SRELVNWAAQLPTNARGVNTGTGGGNAANVCGGGPVSSGFGWPA
                     DDHTLSGNAYGPGHLGIDISAPEGANVYAAGTGVVTMAAGGWNYGYGNVVQIDHGNGY
                     VTVYAHLSSILVSQCQTVGQGSVIGLAGNTGNSFGAHLHFEIRVGGSNINPYDIVQ"
     gene            526..1305
                     /locus_tag="PKI33_01315"
     CDS             526..1305
                     /locus_tag="PKI33_01315"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNM35665.1"
                     /translation="MNESSLKKRLAKLNLGGLRYFESLGSTNDEALAWAARGADDFSL
                     VVADEQTQGRGRLDRKWFTPKGSAIAMSLILRPTAVMRSHLSRTVGLAALSLADACLK
                     LGLSPKIKWPNDILLDSKKTAGILVEMVWAGEDADSLVIGMGINVHKSAVPPRETLQF
                     PATSLEDMLGNAPKREDIIFQILSNVQRWREQIGTERFIQAWEEKLAFRGEAIQIHAG
                     GAESHQGVLDGLESDGSLRICDEHNKSVIVRFGDVSLRPTA"
     gene            1336..1560
                     /locus_tag="PKI33_01320"
     CDS             1336..1560
                     /locus_tag="PKI33_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35666.1"
                     /translation="MFDDLRNDAAKQYEDEVQAEYQPASVTSSGAPRQRSTKFLGMTS
                     IQRFILVFLLMTATCVIGFLCLFAFGKIAF"
     gene            1612..2376
                     /locus_tag="PKI33_01325"
     CDS             1612..2376
                     /locus_tag="PKI33_01325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNM35667.1"
                     /translation="MQILNTLNFFTSKNLFKSYKRLGFWQTIILFSGVAFVSVTGIIV
                     PTNMLLGGSIRTGMVINFIVCTTFMPYHLHQILSLMVELDTLRMNIYDKSIRDELTKA
                     YNRRYFFEASKAIENNEALIPQNTSLLLIDIDDFKLLNDTYGHDIGDLALKTLTEESS
                     RLLRTTDVFARYGGDEFICLLPNTKQSQAYEIATRLLESANKHPFYKKDRNIFLRLSI
                     GIATTTSETKLNDLISLADKALYKSKQNGKNTVEAL"
     gene            complement(2415..4931)
                     /gene="gyrA"
                     /locus_tag="PKI33_01330"
     CDS             complement(2415..4931)
                     /gene="gyrA"
                     /locus_tag="PKI33_01330"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNM35668.1"
                     /translation="MAEENMIAGNVESVDIDAQMRSAYLDYAMSVIVARALPDARDGM
                     KPVHRRILYAMHELGLRSNSSYKKSARIVGEVLGKYHPHGDSAVYESMARMAQDFSMR
                     YMLVDGQGNFGSVDGDAPAAMRYTEARLQKMAEEMLQDLEKETVDYGPNFDDSLQEPL
                     VLPARLPNLLLNGASGIAVGMATNIPPQNLRELTSAINYIIDNYDKADEITVDDLMKF
                     VQGPDFPTGGMIVGTEGIVSAYGTGRGRIVMRGIAHIEETKAGRYQINITEIPYQVNK
                     SSLIERIAELVREDKIDQISDLRDESDKRGMSIVIELKRGAQPKKVLNQLYKYTALQS
                     TFGVQMLALVDGEPRTLPLKRLLQIFIEHRQIVITRRSQFDLAKAKARLHILDGYLIA
                     LNNIDAIIKVIREAEDADKAKSALITRFKLSEIQAQAILDMQLRRLAALERWKIEEEH
                     KQVSETIKYLEDLLANPHKILALIKDDMTDVAEKYGDDRRTKISAEAREDIHEEDLVP
                     DESVLISLTERGYIKRVAATAFRQQSRGGRGVKGHTTKDEDEVVMLIPARSLDTMLFF
                     TDKGKVYSEKVYQIPDLDRAQKGIPLVNILALGPNEKVTAAMSVRDFLPTQFCIMMTG
                     RGRIKRVTMEEFASVRPSGLIAMNLDEGDTLGWVRLTSGKDEIIIVTENGQALRFSED
                     KVRAMGRQAAGVQGIRLGKEDAVTSMDVVDKEGALLVVTTNGFGKQTPLKEYTPKGRA
                     TGGISTIDQKALQEIGKIAAARVVNKDDDLTIMTANGVAIRLKVKDVKQSGRATRGVH
                     LIKPQEGDSVASVARISADDLKKAGAQIEEQEAEPQPKLV"
     gene            complement(5202..5285)
                     /locus_tag="PKI33_01335"
     tRNA            complement(5202..5285)
                     /locus_tag="PKI33_01335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5249..5251),aa:Leu,seq:cag)
BASE COUNT         1579 a         1502 c         1438 g         1619 t
ORIGIN      
        1 gttctcgtga gttggtgaac tgggctgcac aattgcccac caatgcacgc ggagtgaaca
       61 ccggcaccgg cggtggcaat gcagccaatg tgtgtggtgg tggacctgta tctagcggct
      121 ttggttggcc cgccgatgac cacaccctct ctggcaatgc ttacggacct ggtcaccttg
      181 gcattgacat cagcgcccct gaaggtgcca atgtgtatgc cgctggcacc ggtgttgtaa
      241 ccatggcggc tggcggttgg aattacggtt atggcaacgt agtgcagatc gatcacggca
      301 atggttacgt aacggtttat gcccacttaa gctccattct cgtcagtcaa tgtcaaaccg
      361 tggggcaagg ctcggtgatc gggctggcag gcaacacggg taactccttc ggcgcgcact
      421 tgcactttga aattcgtgtc ggtggttcga atatcaaccc gtacgatatt gttcaataaa
      481 atagagacct gacagtttca aagcctgtca ggtcttttaa tttccatgaa cgaatcctct
      541 ctcaaaaaac gacttgcaaa actaaacctc ggcggcttgc gctattttga atcgctgggc
      601 tctactaatg acgaagctct tgcatgggca gctcgtggcg cggatgattt ctctctcgtc
      661 gtggcagatg aacaaactca agggcgtggg cggttagaca ggaaatggtt cacgcctaaa
      721 ggcagtgcga ttgcgatgag tctcatccta cgccccactg cggtgatgcg ctcgcatctc
      781 tcgcggaccg tgggcttggc agctctctca ttggcagatg cttgtctcaa gctgggtctg
      841 tctcccaaga tcaagtggcc caatgatatt ctgctcgata gcaaaaagac tgcaggcatc
      901 cttgttgaaa tggtgtgggc tggcgaagat gccgattcat tggtaattgg gatgggaatt
      961 aacgtccata agtctgcagt gccgccaaga gagacgctcc aattccctgc aaccagtttg
     1021 gaagatatgc ttgggaacgc tccgaagcga gaagatatca tctttcagat tttgagcaat
     1081 gtgcaacgct ggcgtgaaca gatcggcaca gagcggttca ttcaggcgtg ggaggaaaaa
     1141 ttagcctttc gcggagaggc aattcaaatc cacgctggag gcgcagagtc tcatcagggt
     1201 gtgttggatg gtttggagtc tgatggtagt ttgcgcatct gtgatgaaca taacaaatct
     1261 gttattgtgc gctttgggga tgtgagtctg cgcccgactg catgatatac ttttaaaaaa
     1321 taatcagagg ttcccatgtt tgatgatctt cgaaatgatg ccgcaaaaca atatgaagac
     1381 gaagtacagg cagaatatca gccagcttca gtcacttcat cgggtgcgcc gcgccaacgt
     1441 tcaaccaagt tcctcggcat gacatccata cagcgtttta tacttgtgtt cttattgatg
     1501 accgcaacct gtgtgattgg ttttttgtgc ctttttgcct ttggaaaaat cgctttctaa
     1561 gtcgcaacgc atttaacaat agggtgttta gtatctgcat agagtgatag catgcaaata
     1621 ctaaacaccc tcaatttttt tacaagcaaa aatcttttca aaagttataa acggctggga
     1681 ttctggcaaa cgatcattct tttttcgggg gttgctttcg tttcagtaac aggcattatc
     1741 gtccctacca acatgctctt gggcggaagt atcagaacag gcatggttat caacttcatt
     1801 gtttgtacaa ccttcatgcc atatcacctt caccaaatcc ttagcctcat ggtggagcta
     1861 gacactttaa gaatgaacat atacgacaag tcgattcggg atgagttgac gaaggcatat
     1921 aaccgccgct acttttttga agcgtctaag gctatcgaga acaacgaagc cttaattccc
     1981 caaaatacat ctctgctatt aattgatatt gatgatttca aattgttaaa tgacacctac
     2041 gggcacgata ttggcgacct tgcgttaaag acgcttacag aagaaagctc gcgtctgttg
     2101 cgtactacag atgtctttgc acggtatggt ggagatgaat ttatctgcct gttgcccaac
     2161 acaaagcaat ctcaagccta tgagatcgca acccgcctgc tcgaaagcgc gaacaaacat
     2221 cctttctaca aaaaggatag aaacattttt ctccggctta gtattgggat tgccacaaca
     2281 acaagcgaga ccaaacttaa tgacctaatc tctttagccg ataaagcttt atataaatca
     2341 aaacaaaacg gaaagaatac tgtagaagcc ttataaaaaa gacctagtga tttctcacta
     2401 ggtctttttt atcgttatac caacttcggc tgtggttcag cttcttgctc ttcgatttga
     2461 gcaccagcct ttttcagatc atcggctgag atgcgtgcca ccgacgccac actgtcacct
     2521 tcttgtggct tgatgagatg cacgccgcgg gttgcccgcc cagattgttt cacatcttta
     2581 accttcaaac gaatcgctac accgttcgca gtcataatgg tcaggtcgtc atctttgttg
     2641 accacacgtg ccgctgcaat cttgccgatc tcttggagcg ccttctggtc aatggtggaa
     2701 ataccgcccg tagcacgtcc tttgggtgta tattctttca aaggtgtctg cttcccaaat
     2761 ccatttgtgg tcacaaccag caatgcgcct tctttatcca ccacatccat gctagtgacg
     2821 gcatcttcct tgccaaggcg aatgccttga acacccgcgg cttgacgccc catcgcacgg
     2881 actttatctt cactgaatct caaggcttgt ccattttcgg tgacgatgat gatctcatct
     2941 tttccagagg tgaggcgaac ccagcccaga gtgtcccctt catctaagtt catggcaatc
     3001 aagccagagg ggcgaactga ggcgaattct tccattgtta cacgcttgat acgcccacgt
     3061 ccggtcatca tgatgcagaa ctgggtcggc aagaaatcgc ggaccgacat tgccgctgtc
     3121 accttttcgt tcggtcccag cgcaaggatg ttgaccaacg gaataccctt ctgagcacga
     3181 tcgaggtctg ggatttgata caccttttcg gagtacacct tacccttatc ggtaaagaac
     3241 agcatcgtgt cgaggctgcg agcagggata agcatcacaa cttcgtcttc gtctttggtt
     3301 gtgtgcccct ttacaccacg tccgccacgg ctttgctggc ggaaggcagt tgctgccaca
     3361 cgtttgatgt atccacgctc ggtgagactg atgagaaccg attcatcggg cacaaggtct
     3421 tcttcgtgaa tatcttcgcg agcttcggca gaaattttgg tacggcggtc atcgccatat
     3481 ttttctgcta catcggtcat atcgtctttg ataagagcga ggatcttatg cgggttggca
     3541 agcaagtctt ccaaatattt gatggtttca gaaacctgct tgtgttcttc ttcgatcttc
     3601 cagcgttcga gagctgcaag gcgacgtaac tgcatgtcga gaattgcctg tgcttgaatt
     3661 tcgctcaact taaagcgggt tatcaacgca gacttggctt tgtctgcatc ttctgcttca
     3721 cgaatgacct taataatggc gtcaatgtta ttgagcgcaa taagatagcc atccaaaata
     3781 tgcaagcggg ctttggcttt ggcgaggtca aactgtgagc ggcgggtgat gacgatctgg
     3841 cgatgttcaa tgaagatctg caacagacgc ttgagcggca aagtacgcgg ctccccatcg
     3901 accaatgcca gcatctgcac cccaaacgtg gactgcaaag ctgtgtattt ataaagctgg
     3961 ttcaaaacct tcttaggctg agcaccacgc ttaagctcga tcacaatgct cataccgcgc
     4021 ttgtctgatt cgtcgcgcag gtctgaaatt tggtcaatct tatcctcgcg gacgagttca
     4081 gcaatgcgtt cgatcaggct ggatttattg acctgatacg gaatttcggt gatgttaatc
     4141 tgataacgcc cggctttggt ctcttcaatg tgggcaatgc cgcgcatcac aatgcgtccg
     4201 cgcccggtgc catacgccga gacaatccct tccgtcccca cgatcatgcc gccggttggg
     4261 aagtctggtc cttgcacgaa tttcatcaaa tcgtcgacgg taatctcatc ggctttatcg
     4321 tagttgtcga taatgtaatt gatcgcacta gtcaactcgc gcaggttttg tggcggaata
     4381 ttggtcgcca tacccacagc aatacctgaa gcgccattca acaacaaatt cggtaagcga
     4441 gcagggagaa cgagcggttc ttgcaaagaa tcatcgaagt taggaccata atccacggtc
     4501 tctttttcga ggtcttgcaa catttcttct gccatctttt gcaggcgagc ttcggtataa
     4561 cgcatcgcag ccggcgcatc cccatccacc gaaccgaagt taccctgacc atccaccagc
     4621 atgtaacgca tcgagaaatc ttgcgccata cgcgccatcg actcatacac agccgagtca
     4681 ccatgcgggt gatatttacc cagcacttca cccacaatac gagcagactt tttataagac
     4741 gagttggagc gtagccccaa ttcatgcatc gcatacaaaa tgcgacgatg caccggcttc
     4801 ataccgtcac gggcatcggg caaggcacgc gccacgatca cgctcattgc gtaatcgagg
     4861 tatgccgagc gcatctgggc atcaatatct acagactcga catttccagc gatcatattc
     4921 tcttcagcca taaattatcc tcttgagggc aaaaacgagc gaatttccgc ctttttcaca
     4981 aaaaaatgac ataaaaagga cacgaagacg ggaagaactt cgtgtcacat tagatgaaga
     5041 aagcgtaatt ttatagtgca attataccac ccctatccgc tttttgcctt gcagattaag
     5101 catccagcct agggcaagcc caatgggata aaacccgcct cctcccccgt ccgagccggg
     5161 ttctatccat caaaaaagac ccgcagctgc gagtctttgt gtgccgaagg agggacttga
     5221 accctcatga gattgctctc actacgccct gaacgtagcg cgtctgccaa ttccgccact
     5281 tcggcatatc atctgaatgg cgggatgtat tttatcccaa acgggtggaa tgtcaataag
     5341 agggtgttga tgttcaattt tatgtgaaca atatcacagc acaaatataa gatgatgatg
     5401 ttgtatttag tagtgaaaac cccactgtag tattggaact atttatgata aaagtgcgtt
     5461 aatagaatat acctttcaag agtgtattgt caaaccatta cagtactgaa aggggagtat
     5521 ctttctaaaa aaagaatcgt tatatttgat atagaaaaag aaaggagttg cacaatgttc
     5581 gtttttgccg cagtggttgt tgtactactg ctcgccctgg caagctctga tatgttcctc
     5641 gcgcaataca gccaggatga actcagcaac atgggagttc aagagcaatg atggatgacc
     5701 accccatccc ctagcccaac gcatttgatc gaacgcgttg ggctttttct tttttaagcg
     5761 gggaaatagt tcaagcgagc gagaacatcg ctggcttcca ttaaaagata atccacaatt
     5821 agatttattg aatagatgta ggcgtctaaa ataagtgaca ggcgagatat taatgttaca
     5881 tatgtatttg ggatcctacc gctatttcaa aagggtgtga ctttcaaact ccctaccgct
     5941 cggtagggaa acaaagtttg agatataata cgcgcacgag agaatgtagt tcgaaacagc
     6001 acccatccaa cgccctcgaa tcacgcttcc acaaggatcg cccatgcaga tcactccgct
     6061 tcacaccagt attgaaaagc ttattgagtt gaaaataggt gttgtcgtga tggaaagccc
     6121 aaatggcttc ccattaca
//
LOCUS       DAOREF010000038         6126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6789_length_6126_cov_5.094218, whole genome shotgun sequence.
ACCESSION   DAOREF010000038 DAOREF010000000
VERSION     DAOREF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6789_length_6126_cov_5.094218"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..644)
                     /locus_tag="PKI33_01340"
     CDS             complement(15..644)
                     /locus_tag="PKI33_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35669.1"
                     /translation="MSKLTPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYKYHSPLV
                     SGEWCAFTNFPWGRGLINFEPQEESLAMETYNTWVKLFELQKYYSWIVDRFHLSTRMY
                     QLMTYQKDYDFRWLEERLLPLNFRLVLMTRSPESFAAARAERLKVSGNPSQYDDLNLF
                     VEEQKMIRKLFNDSILPKIEIDISDNNISAAVEHIANWMESTGGLYMAE"
     gene            complement(641..1513)
                     /locus_tag="PKI33_01345"
     CDS             complement(641..1513)
                     /locus_tag="PKI33_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM35670.1"
                     /translation="MKKLAFLSLLLVSSLACATIEKALTSGATPTPIVIPTIAPSQTA
                     VPIATDIIRKEESDIFCPSNVTEAVDAYNKGLTYENAGDTASAEKAYRDAIALDPNYC
                     DAMDNLALLVRKNGNSDEAIALYENSIKVAPDNQVAHLGLANTYMELEEYDKALAEYN
                     AILAINPENPEGYYGAGRVYFSKEDFKSAIPQFKQAEELYKAEGSSYLIDAQVYLGFS
                     YALIDEFETGRDYLEMVYPQMQDNGYVNYFLGYCYYYGTSIRDEKLAKQYLLRARDLG
                     IDLGSDLETFVNTP"
     gene            1659..2024
                     /locus_tag="PKI33_01350"
     CDS             1659..2024
                     /locus_tag="PKI33_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35671.1"
                     /translation="MDDKITIIEGPPPVFEHVNDGWAMGLNESPNLSIPALTRLRTFN
                     GPALVQRCYNAWHNKTSIHLHYRNETGLEQTAPILAARNVETPEGHVLLLWVYLDSEK
                     VEYETDDASDDDLDDYPGE"
     gene            2089..2862
                     /locus_tag="PKI33_01355"
     CDS             2089..2862
                     /locus_tag="PKI33_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNM35672.1"
                     /translation="MTTTLLSTLKSGVLTLSLNRPERANAFNFEMTTALQSALTDAEK
                     DSQVRCVVLTGNGKVFSAGQDISEMKKGGGEISYAEHLEKTYNPLILQIREMGKPVVA
                     AVNGACAGAAFGIALACDLRIAHTNAYFVVGFSGIALAPDSGVSLLLPKLIGLGRAQE
                     YFYTNSPIPAQLAFNWGIVNRLGGFNYQRVVDQMVGDLAQGPREAFALGKKAFNEAVL
                     PNLASVLHGEGILQDVAGKSSEHQVAVKAFFEKRKPKYD"
     gene            3087..4385
                     /locus_tag="PKI33_01360"
     CDS             3087..4385
                     /locus_tag="PKI33_01360"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNM35673.1"
                     /translation="MSGQINAFEMAQKQFDAVAKQLKLDSGVAETLRWPMREYAFRIP
                     VRMEDGSLKVFQGFRVQHNDARGPNKGGIRFHPAETLDTVRALATWMTWKCAVADIPL
                     GGGKGGIIVDPSTLTVREKEDLCRGWVRNMWKNIGPRNDVPAPDVGTTPQMMGWMMDE
                     YSKLVGHYTPGVFTGKPLGGGGSLGRTEATGYGVIYTVREAMKRLNIDPKKSVAVLQG
                     FGNVSQYAAIGFVEILGGTVACVSCYDRHDKKAYTYSKKGGIDPRFLLSIVDEYGTID
                     KKKAMDSGYEVSDGDKWIEFEGNVLIPAALEGQVNGETVQKVSKSVKIVAEGANGPTT
                     PEADEVFKKNGVFNIPDFLCNAGGVTTSYFEQVQNDMNFYWSKDEVLQRLDEKMTSAF
                     NSVFDRSEKEKVYMRDAAYMVAIDRVVKAMELRGWLTGSA"
     gene            4544..5719
                     /locus_tag="PKI33_01365"
     CDS             4544..5719
                     /locus_tag="PKI33_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNM35674.1"
                     /translation="MSKQTFKWDDPFLLNDQLTDEERMIRDTARKYCQDKLMPRILEA
                     NRHEVFHREIMTEMGNMGFLGSTIPEEYGGAGLNHVDYGLIAREVERVDSGYRSAMSV
                     QSSLVMYPIFTYGTEEQRKKYLPKLASGEWVGCFGLTEPNHGSDPASMETKAKKVDGG
                     FILHGNKMWITNSPIADVFVVWAKLDGEIRGFILEKGMKGLSAPKIEGKFSLRASATG
                     EIVMDEVFVPAENIFPEVKGLKGPFGCLNKARYGIAWGALGAAEFCWHAARQYTLDRP
                     QFGRPLAANQLMQLKLANMMTEITLGLQGALRVGRLIDEDKVSPEMISLIKRNSCGKA
                     LEIARTSRDMHGGNGISDEYHIIRHVMNLEAVNTYEGTHDIHALILGRAQTGIQAFS"
     gene            complement(5760..>6126)
                     /locus_tag="PKI33_01370"
     CDS             complement(5760..>6126)
                     /locus_tag="PKI33_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35675.1"
                     /translation="TLAEKDGENPPPGFPGLETLLPLLLQGVQDRRLTIDQIVEKLYT
                     NPKRIFNLPEQPETWVEVDEDVTYEIKASESFTRCGWTPFEGWKVKGKVRKVVLRGNT
                     AFEDGKVFAEAGSGKNIRG"
BASE COUNT         1519 a         1469 c         1562 g         1576 t
ORIGIN      
        1 taccgatgaa gtctttattc tgccatgtac aacccgcccg tggattccat ccaattggcg
       61 atgtgttcta cggcagccga aatgttgtta tctgaaatgt cgatctcgat ttttggcagg
      121 attgaatcat tgaacaactt gcggatcatt ttctgttctt ccacaaagag gtttaagtca
      181 tcatattgtg agggatttcc agagaccttc aagcgctcgg ctcgagctgc ggcaaaagat
      241 tcgggtgagc gcgtcatcaa gacgaggcga aaattgagcg ggaggagacg ttcttccagc
      301 cagcggaagt cataatcttt ttgatacgtc atgagttgat acatgcgtgt ggaaagatga
      361 aaacggtcca cgatccatga atagtatttt tgcaactcga agagtttgac ccaagtattg
      421 taggtctcca ttgccagtga ctcttcctgc ggctcaaagt tgatcagccc gcgcccccag
      481 gggaagttgg taaaggcgca ccattctcca gaaaccagcg gcgagtgata cttatacttg
      541 cgcggaccga caatgcgtgg gtgttcgttg agtgcaaacg caatatcggt tttgaaggtc
      601 aggcgtgtcc cttcgaggat gatcttcggt gtgagtttgc tcatggtgtg ttgacgaaag
      661 tctccaagtc tgatccaagg tctatgccga ggtcgcgggc acgcagcagg tattgttttg
      721 ccaacttctc gtcacgaatg gatgtgccat aataataaca gtagccaagg aagtagttaa
      781 catagccgtt atcttgcatt tgtgggtaaa ccatttcaag ataatcgcgc ccggtttcga
      841 attcatcgat caatgcgtat gaaaaaccga gataaacctg tgcatcgata agatagctcg
      901 acccttcggc tttataaagc tcttctgctt gtttaaactg aggaatagca gacttgaaat
      961 cttctttgga gaaatacacc ctgcctgccc cgtaatagcc ttctgggttt tctgggttga
     1021 tcgccaagat cgcattgtat tcagccaatg ctttatcgta ttcttcaagt tccatgtagg
     1081 tgtttgccaa ccctaagtgc gctacctgat tatcgggtgc tactttgatg gagttttcat
     1141 acaaagcaat cgcctcatct gaatttccat ttttgcgtac caaaagagcc aggttatcca
     1201 tggcatcgca ataattcggg tccaatgcta tggcatctcg ataggctttt tctgctgagg
     1261 cagtatcgcc tgcgttttca taggtcaacc ctttgttgta ggcgtccaca gcttctgtca
     1321 cattggatgg acaaaaaata tcagattctt cttttcgaat gatatcggtg gcaatgggca
     1381 ccgcagtttg ggatggggca atggtaggga tcacaatagg cgtgggcgtt gcgccggaag
     1441 tcaacgcttt ttcaatcgtg gcacatgcca gcgaacttac caaaagaaga gataaaaaag
     1501 ccaatttttt catcatttgc ctccagtttg tatgtgaaaa ttatctcatt caatgcatcc
     1561 agaacctata gtctatttgt cctgatttcg attctgataa atttctgaaa cgattcgaca
     1621 acccagttgt ggtatattcc cgccaaggta aatttattat ggacgacaaa attacgatca
     1681 tagaaggtcc gccccccgtt tttgagcacg tcaacgacgg atgggcaatg ggcttgaacg
     1741 aaagcccgaa tctctccatc cccgcgctta cgcgcctgcg cacgtttaat ggtcccgcct
     1801 tggtgcagcg ctgttataac gcgtggcata acaaaacttc catccacttg cattaccgca
     1861 acgaaacggg cttggaacaa accgctccca ttcttgcagc ccgcaatgtg gaaacccccg
     1921 aagggcacgt cttgttgttg tgggtctatc tcgatagcga aaaggtcgaa tacgaaaccg
     1981 acgacgccag cgacgacgat ttagacgatt accccggcga ataaagttga ttacaatcag
     2041 tgacagtcat caagaaggtg actgtcactt ttgtttatag gattaaccat gaccactacg
     2101 cttctttcca cgctcaaatc tggtgtgctc accctgagcc ttaaccgccc cgaacgcgcc
     2161 aacgccttta actttgaaat gaccacagct ttgcaaagcg cgttgaccga tgccgaaaaa
     2221 gattcacaag tgcgttgtgt tgtccttact gggaatggca aagtattcag cgccgggcaa
     2281 gatatttctg aaatgaaaaa aggcggcggg gagatttcgt atgcagaaca tttggagaaa
     2341 acctataacc cactcatttt acaaattcgt gaaatgggca aacctgttgt cgctgcggtt
     2401 aatggagcgt gtgcgggcgc tgcctttggc atcgctttag cctgtgactt gcgtattgcc
     2461 cacaccaatg cgtattttgt ggttggtttt agtggcattg ctcttgcgcc cgattctggc
     2521 gtttcattgt tgttaccaaa actgatcggg ttggggcgtg ctcaagaata tttctatacc
     2581 aatagcccca ttccggcaca attggccttt aattggggca ttgtgaatcg gctaggcgga
     2641 tttaactatc agcgtgtggt agaccaaatg gtgggcgacc tggctcaagg tccgcgtgag
     2701 gcgtttgcgt tggggaagaa agcgttcaat gaggcggtcc tgcccaactt ggcaagtgtc
     2761 ttgcacggcg agggcatcct gcaagatgtt gcgggaaagt cgtctgaaca tcaggtggcg
     2821 gtaaaagcgt tctttgagaa gcgtaaaccg aaatatgact agcgtatcct atttgggggc
     2881 aacagggtta ttacatggcg tcagaaatca gcgtactttt gtcatattga cgcaaaaatg
     2941 aatctcttgt atagttttag agtagtattc gtggaattac cgatcttatg aaccagcgtt
     3001 cttacatagg ctgtgaataa gcgggcaccc cgtccggagt gcccgcttat attttctctt
     3061 aaacctctac ctaggagagt aaaaaaatgt caggacagat caatgcgttc gaaatggcac
     3121 agaagcaatt tgatgctgtt gccaagcaat tgaagctcga ctctggcgtg gctgaaaccc
     3181 tgcgttggcc gatgcgcgaa tatgcgttcc gcatccccgt tcgcatggaa gatggctcgc
     3241 tcaaggtctt ccaaggcttc cgtgtgcagc ataacgatgc gcgcggaccc aacaaaggtg
     3301 gtattcgctt ccacccggct gagacgctcg atactgtgcg cgcgctcgcc acatggatga
     3361 cctggaaatg cgccgtggca gatattccgc tcggcggcgg taaaggtggc attattgttg
     3421 acccctccac cctcaccgtg cgcgagaagg aagacctttg ccgtggttgg gtacgcaata
     3481 tgtggaagaa tatcggtcca cgcaatgacg tgcccgcccc cgatgtaggt accactccgc
     3541 aaatgatggg ctggatgatg gacgaatatt ccaaactcgt cggtcattac acccctggcg
     3601 tcttcactgg caaacccctt ggcggcggcg gctcgctcgg ccgtaccgaa gccacgggtt
     3661 atggtgtcat ctacaccgtc cgcgaagcga tgaagcgcct caacatcgac ccgaagaaat
     3721 ctgtggctgt gcttcagggc ttcggaaatg tgtcgcagta tgcggcaatc ggtttcgttg
     3781 aaattctcgg tggtaccgtg gcttgtgttt cttgctacga ccgccatgac aagaaagcct
     3841 acacctatag caagaagggc ggcattgatc cgcgcttcct gctctccatc gtggatgaat
     3901 acggcaccat cgacaagaaa aaggcgatgg actccggtta cgaagtcagc gatggcgata
     3961 agtggatcga gttcgaaggc aatgtgctca tccctgccgc actcgaagga caggtcaacg
     4021 gcgaaacggt gcagaaggtt tccaagagcg tcaagatcgt tgctgaaggc gcgaatggtc
     4081 ctaccactcc ggaagccgac gaagtgttca agaagaacgg cgtgttcaac attccagact
     4141 tcctctgcaa cgctggcggc gtgaccactt cttacttcga acaggttcag aacgatatga
     4201 acttctactg gagcaaggat gaagtcctgc agcgcctcga tgagaagatg acctctgcct
     4261 tcaacagcgt gttcgatcgc agcgaaaagg aaaaggtcta catgcgcgat gcggcttaca
     4321 tggtcgccat cgaccgcgtg gtcaaggcga tggaactccg tggttggttg acgggttctg
     4381 cgtagttagt acgatgtgat gtataagagg atgtgcctga aagggtgcat cctctttttg
     4441 attaacgctt cattcttagc cgatgcttgc ttctgttttc tgacacagtt ttctacccct
     4501 tgcttttgat acaatcaccc tatccctaat ccccgaggtc ctcatgtcca aacaaacttt
     4561 caaatgggac gatccctttc tgctcaatga ccaactgacc gacgaagaac gcatgatccg
     4621 tgacacggcg cgcaagtatt gtcaggataa gctcatgccg cgcatcctcg aagcgaatcg
     4681 acatgaagtt ttccatcgcg aaattatgac cgagatgggc aacatgggct tcctcggttc
     4741 gaccattcct gaagaatatg gcggcgctgg cttgaatcac gtggactatg ggcttatcgc
     4801 ccgcgaagtg gaacgcgtgg atagcggcta tcgttctgcg atgagcgtac agagttcgtt
     4861 ggtgatgtat cccattttta cttatggcac cgaagaacag agaaaaaaat atctgccaaa
     4921 actcgccagt ggagaatggg ttggctgttt cggcttgact gaacccaatc acggcagtga
     4981 ccccgccagt atggaaacca aagccaaaaa agtagatggc ggttttattc tgcacggcaa
     5041 taagatgtgg atcacgaact caccaattgc agacgtcttt gtcgtttggg caaaactcga
     5101 tggcgagatc cgcggcttta tcttggaaaa aggcatgaaa ggactctctg ctcccaagat
     5161 tgaagggaag ttcagtctgc gggcgagtgc caccggcgag atcgtaatgg acgaagtatt
     5221 cgttccggca gagaatatct tccccgaagt gaagggactg aaaggtccgt tcgggtgttt
     5281 gaacaaggcg cgctatggca ttgcttgggg cgcgcttggt gcggcagaat tttgctggca
     5341 tgcagcaaga caatacacct tagaccgtcc gcagttcgga cgtccgctcg cggcgaatca
     5401 gctcatgcag ttgaagcttg cgaacatgat gacggaaatt acgttgggat tacaaggtgc
     5461 attacgtgtt gggcgtctca tcgacgaaga caaagtttcg cctgaaatga tctcacttat
     5521 caaacgcaac tcctgcggca aagccttgga gattgcccgc acgtcacgcg atatgcacgg
     5581 cggcaacggt atctcagatg aatatcacat cattcggcat gtgatgaatt tggaagcggt
     5641 caacacctat gaaggcacac acgatattca tgccttgatc ttaggcagag cgcaaacagg
     5701 aatacaagcc ttttcttaga tgaaaaaaga atggacgggt ttctcccgtc cattcttttt
     5761 tatcctctta tattcttgcc agagccagct tctgcgaaaa ccttgccatc ttcaaaagca
     5821 gtattccctc gtagcaccac tttacgaact ttccctttta ctttccatcc ttcaaaggga
     5881 gtccagccgc agcgggtaaa actctcgctg gctttgattt cataagttac atcttcatcc
     5941 acttccaccc aggtttcggg ttgttcgggc aggttgaaga tgcgtttggg gttggtgtaa
     6001 agtttttcca caatttggtc gattgttaat cttctatcct gcacgccttg taacaacaag
     6061 ggaagtaagg tctccaaacc ggggaagccg ggcggtggat tctcgccatc tttttcagcc
     6121 aaagta
//
LOCUS       DAOREF010000039         6093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6864_length_6093_cov_4.389202, whole genome shotgun sequence.
ACCESSION   DAOREF010000039 DAOREF010000000
VERSION     DAOREF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6864_length_6093_cov_4.389202"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..412)
                     /locus_tag="PKI33_01375"
     CDS             complement(8..412)
                     /locus_tag="PKI33_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNM35676.1"
                     /translation="MPTLKDIEGIGPVNAGKLNKAGVRGLNGLLKLGASRKGRQEIAK
                     ATGINAKVILEWVNRADLFRVKGIGGQYSDLLEAAGVDTAVELSKRQPEALLEAMKKA
                     NAKLNKVNQMPGLSNVKTWIKNAKSLKRVIEY"
     gene            599..1087
                     /locus_tag="PKI33_01380"
     CDS             599..1087
                     /locus_tag="PKI33_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35677.1"
                     /translation="MPTINFNGKTYNNIEEMPANERQAFEKLSSLLVDANGNGIPDLL
                     EGDMVQNVMAVHATKTSINVNGQTYHSLEDLPPDLRQSVDGAFQFLSKMGIANTTSDI
                     QTPAMRQEAQPKSKPFLAQESPSAIEEESGRSTFSIVLISIVLCFAVVAATIAIFVFI
                     NR"
     gene            1084..1476
                     /locus_tag="PKI33_01385"
     CDS             1084..1476
                     /locus_tag="PKI33_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35678.1"
                     /translation="MKLVLIYTLIAGVISAITAPIPGTSLLLTALEVYMIVHLSKSNE
                     SKLGFKEIGYSAMALYGLSTVLKDAALEILTFVPLIGWGAEVIVAMLFVFFLGMLANL
                     YFSKPSRKGRNLENNPDEISSHSTDRPN"
     gene            complement(1405..2145)
                     /locus_tag="PKI33_01390"
     CDS             complement(1405..2145)
                     /locus_tag="PKI33_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNM35679.1"
                     /translation="MDTETSRTLHEELIHHNNVDPHKRGSGLSDFILGAQDGLVNVLG
                     VVLGIAAATNDARVVLVAGLATTFAESISMGAVAFTTTLADADLYQSEREREYRHIAE
                     APMLETKEIRDIYASKGFTGDLLDRIVETITANKDVWVAVMMAEEHQLSPVDRGTAMK
                     AAWVVGLSAIIGSLVPILPFIFLPVSISMWLSVLVTALVLFGIGFYKARVTVGKPMRS
                     GTEMMLIGTISAMAGYLVGVVLKVPPLP"
     gene            2187..3194
                     /locus_tag="PKI33_01395"
     CDS             2187..3194
                     /locus_tag="PKI33_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNM35680.1"
                     /translation="MSRIRLIVILCLTALACQLPSIPILATQTPTQTPTATVTSTPTQ
                     TLTPEPTLTPSITPTPTPLARRVLILSIDGFRPDAIPLAPMPFLTSLLSQSAYSLTAQ
                     TVYPSVTLVSHTSMLSGQCPAKHKVDWNDYIPENGYAQVTDLFDVVHAAGMQTVMYVG
                     KEKLRQITEPQSTDIFKFINDRDLVITDNLIENFPTDFRLMFVHFPTTDWMGHEYGWL
                     SGEQLSVLFRADQALEKLVTELNNRGLMSETLFIITADHGGHATTHGTSLYEDMTIPW
                     IAFGAGIKPAALNSSITTVDTAATAAFALGLNIPTEWDGVPVYEAFGLPIEKASVGCS
                     K"
     gene            3215..3880
                     /locus_tag="PKI33_01400"
     CDS             3215..3880
                     /locus_tag="PKI33_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNM35681.1"
                     /translation="MERILEPELMEDMEQALAYSHADFATSHQMRVTWFQERFGKDNQ
                     PASILDLCCGGGDMTFRFARAFSHSHIIAVDGSQAMLDIAQKDVQAEPEIANRIQFVR
                     AFLPSDEIPQNKYDLVMSHSALHHFHDPNALWKTIRAHTRPGTLVFISDLQRTESVEA
                     AEQLVRERAGNEHPILQKDFAASLCAAFTPEEVREQLNANGLDALKVEAINDVYMLVY
                     GMM"
     gene            complement(3926..4192)
                     /locus_tag="PKI33_01405"
     CDS             complement(3926..4192)
                     /locus_tag="PKI33_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35682.1"
                     /translation="MAEMTCPKCSGAMEEGLQFNFKLNELSDDYVIETAELTRDRWQK
                     TEDATGKFFGKEYLGKRRVGPRLAVITYRCTVCGYLESYAPPVK"
     gene            complement(4260..5072)
                     /locus_tag="PKI33_01410"
     CDS             complement(4260..5072)
                     /locus_tag="PKI33_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35683.1"
                     /translation="MMKRNQPILMFVAVLTIAALACNLPTTSNPADATEEPTEVSTEA
                     PEATPTEAPTEEASDSVSVSVSTATNCRTGPDVVYQLLMTVQPGSDFDVVGKYTPKGY
                     WIINMPTGGTCWLWGQYAIVQGDTSALPEITPPPAPVAQPTSETSNQANNSNTNNNEE
                     EDPQPSAPAPAAPTNTDITRQCTTLTQPGDLFPSFKMVVQFTWNDASDNETGFNIYKD
                     GTFYTTLPANTQKFTETFTVLLAQSPSITYGVQSVNANGSSGLVTGTVSYCK"
     gene            5250..5678
                     /locus_tag="PKI33_01415"
     CDS             5250..5678
                     /locus_tag="PKI33_01415"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNM35684.1"
                     /translation="MQPSDIPNEKTAKRIGNQASFIILALVFSCVVSFFFLFNVDKIK
                     MLQTSIDLQFNGSTTTGTVISLEEFKSSAGHNAVFILTAEFEVDGQPYTVTGNAYYPT
                     LDHSWQGESLDVIYDPQDPNIAMINTFEERWVIPLAGALP"
     gene            5706..>6093
                     /locus_tag="PKI33_01420"
     CDS             5706..>6093
                     /locus_tag="PKI33_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35685.1"
                     /translation="MKSDVIFPLTRIIAGIVVLFLVAAFGILFFLPDQTGTLFAWAIK
                     PHMSSMFFGSAYLGGAWILAQTAFGPRWHRVQAVFPAVTIFTTAMLAATLPHWERFSL
                     GTIPFLAWLILYIVSPFLIPALWIYNR"
BASE COUNT         1579 a         1598 c         1295 g         1621 t
ORIGIN      
        1 tttttattta gtactcaatc acacgtttga gtgatttcgc gttcttgatc caagttttga
       61 cgttgctcaa gccgggcatt tgattaacct tattgagttt agcatttgcc ttcttcattg
      121 cctcaagcaa ggcttctggc tggcgtttgg aaagttcaac cgcagtatcc acaccagcag
      181 cttcgagcaa atcggaatat tgaccaccaa ttccctttac acgaaacagg tctgcacggt
      241 taacccattc aaggataact ttcgcattaa taccagtagc ttttgcaatt tcctggcgtc
      301 ctttgcgaga agctcccaat ttcaagagcc cgttcaagcc ccgaacacca gccttgttaa
      361 gtttgccagc attgacggga ccaattcctt caatatcttt tagtgtaggc atatgttgtt
      421 atctccttat tcaatactat aacatctaat tactaaaaaa ccaattcttt atccattctt
      481 aagcacaaaa gattttattg ttctttccac attatctaaa aactgacgat acaaacttta
      541 cttttaggtt tggtaatatc tgcgtgtaaa atggagagac aagacgcagg agttcatgat
      601 gcccactatt aatttcaatg gaaagaccta caataacata gaagaaatgc ccgccaatga
      661 aagacaggct tttgaaaaat tatccagctt acttgtggat gcgaacggca atggcattcc
      721 cgacctgctt gaaggcgata tggtgcagaa tgtgatggca gttcacgcca caaaaacgag
      781 catcaatgtc aacggacaaa cctatcactc tcttgaagac ttgccaccag atttgcgtca
      841 aagtgtagat ggcgcttttc aattcttatc gaaaatgggt atcgcaaaca cgacgtcaga
      901 cattcaaact ccggcaatgc gtcaggaagc gcaaccaaag tccaagccgt ttctggcgca
      961 agaatctccc tccgcgattg aagaagaaag cggacgtagc accttctcaa tcgttttgat
     1021 cagcattgta ctttgcttcg ctgttgtcgc cgccacgatt gctattttcg tctttatcaa
     1081 ccgatgaaac tcgtcctcat ttacaccctt atcgccggcg tgatctccgc catcactgcg
     1141 ccaatccctg gcacatcctt gctattaaca gcattagagg tgtacatgat cgtacacctc
     1201 tcaaaaagca atgaatccaa attagggttc aaggaaattg gctattcagc catggcactg
     1261 tatggacttt ccaccgtgtt aaaagacgcc gcgcttgaaa ttctcacctt cgttccgctc
     1321 ataggctggg gtgcagaagt gatcgtagcg atgctctttg tcttcttcct tggcatgctg
     1381 gcaaatttat atttcagcaa accatcacgg aaggggcgga accttgagaa caaccccgac
     1441 gagatatcca gccatagcac tgatcgtccc aattagcatc atctctgtgc cgctgcgcat
     1501 gggcttgccc acggtcacgc gcgctttata aaagccgatt ccaaacaaga ccaacgccgt
     1561 caccaatacc gagagccaca tactgatcga aactggtaag aagatgaacg gcaagatcgg
     1621 caccaacgaa ccaatgattg ccgaaagccc aaccacccaa gcagccttca tggcagtgcc
     1681 acgatctaca ggggagagtt gatgttcttc tgccatcatc accgccaccc acacatcttt
     1741 attcgcggtg atcgtttcca caatgcggtc taacaagtcc cctgtaaagc ctttgctggc
     1801 ataaatatcg cggatctctt ttgtctcaag catgggcgct tcggcaatat gtcgatattc
     1861 gcgttcacgc tcgctttgat ataggtccgc atcagcgaga gtggtggtga aagccactgc
     1921 ccccatcgaa atggattccg caaaggtggt tgccaagcct gcgaccaaca ccacgcgtgc
     1981 atcgttcgtc gcggcagcga ttcccagcac cacgccgagg acgttgacca agccgtcttg
     2041 cgcacccaaa ataaagtccg aaagacccga gccacgtttg tgtgggtcca cgttgttatg
     2101 atgaatcaat tcttcatgca aagttcgaga ggtttcagta tccatattca aatcatacta
     2161 cgctgaaagt tatttataat ggacaaatgt cacgcatccg acttattgtt attctttgtc
     2221 ttacggcgct tgcctgccaa ctcccatcca ttcccatcct tgccacccaa acgccaacgc
     2281 aaactcctac cgcaacggtt acatcaaccc cgactcaaac tctcacacca gagccaacac
     2341 taacaccctc gattacgccc acccccacgc ctctcgctcg ccgcgtcttg attctctcca
     2401 tcgacgggtt ccgccccgat gccattccgc tcgccccgat gccttttctc acatcactgt
     2461 tgagtcaatc agcatattca ctaactgcgc aaactgtata ccccagtgtg actcttgtct
     2521 cacacacctc catgctcagc ggacaatgcc ccgccaaaca caaagtggat tggaacgact
     2581 acatccctga aaatggctac gctcaagtga ctgacctttt cgatgtcgtc catgccgccg
     2641 gtatgcaaac cgtaatgtat gtgggcaaag aaaaattacg tcagatcact gagccgcaaa
     2701 gcacagacat cttcaaattt atcaacgacc gcgacctagt catcaccgac aatctgatcg
     2761 aaaacttccc caccgatttt cgtttgatgt tcgttcactt ccctactacc gactggatgg
     2821 ggcatgaata tggctggctt tctggcgaac agcttagcgt gttgtttcgt gccgatcagg
     2881 cgcttgaaaa actcgtgacc gaactcaaca atcgcggttt gatgtctgaa accctattca
     2941 tcatcactgc cgatcatggc gggcatgcca caacgcacgg taccagtctc tacgaagata
     3001 tgaccattcc atggatcgca tttggtgcag gcattaaacc agccgcgctc aattcgtcta
     3061 tcaccacagt tgatactgcc gctaccgctg cctttgccct cgggcttaac attcccaccg
     3121 aatgggatgg tgtgccagtt tatgaagcct tcggtttgcc cattgaaaaa gcatctgtgg
     3181 gttgcagtaa ataaatttat taatagaggt cactatggaa agaattcttg aacccgagct
     3241 gatggaagat atggaacaag cactcgccta ttcacatgca gattttgcca cttcacacca
     3301 aatgcgtgtg acttggttcc aggaacgttt tgggaaggac aaccaacccg cctccattct
     3361 ggacctgtgt tgtggtggcg gcgatatgac ctttcgcttc gcccgagcct tttcacacag
     3421 ccacatcatc gccgtggacg gttcgcaagc catgctcgat atcgctcaaa aagatgtgca
     3481 agccgagcct gaaatcgcaa accggattca atttgtgaga gcttttttac ccagcgatga
     3541 aatcccccag aacaagtatg accttgtaat gtcgcatagt gcattgcatc acttccacga
     3601 cccgaatgct ttatggaaaa ccatccgcgc tcacacccgc ccagggacat tggtcttcat
     3661 ctccgatttg cagcgcaccg aaagtgtgga agccgccgag caactcgtcc gagaacgagc
     3721 cgggaacgag catcccattc tacaaaaaga tttcgccgct tcgttatgtg ccgcgttcac
     3781 ccctgaagaa gtccgcgagc aactcaacgc caatgggcta gatgcgctca aagtagaagc
     3841 catcaacgac gtgtatatgc tggtttatgg aatgatgtaa attacataaa aaaacagctc
     3901 aactaaagtt cgatcaggta gttttttatt ttacaggcgg agcataggat tctaaatatc
     3961 cgcataccgt gcatcgataa gtaataaccg ccaaacgcgg acccacccgg cgctttccta
     4021 aatattcttt cccaaagaat ttacctgtgg catcttctgt cttttgccag cgatcacgcg
     4081 tcagttcagc cgtttcgata acatagtcat ctgatagctc attcaactta aaattaaact
     4141 gtaacccttc ttccattgcg ccagaacatt ttgggcaggt catttcagcc atttttatct
     4201 cctttattga tttaagaaca ccctgtcaaa gaaacagggt gttttataaa accggtcaac
     4261 tacttacaat aactcactgt acctgtcaca agacctgaac tgccatttgc attcacagat
     4321 tgcactccgt aggttataga gggagactgt gccaataaga ctgtgaatgt ctctgtgaat
     4381 ttttgagtat tggcaggcaa agttgtataa aaagttccgt ctttgtagat gttaaagccc
     4441 gtttcgttat cagaagcatc attccatgtg aactgcacta ccatcttgaa gcttgggaag
     4501 aggtcaccgg gttgggtaag ggtggtgcac tgacgggtaa tatctgtatt ggtgggagca
     4561 gcaggcgcag gcgcggaagg ttgcgggtct tcttcctcat tattatttgt atttgaatta
     4621 ttcgcttgat ttgaagtttc actggtgggt tgcgcaacag gagcgggcgg cggtgtaatc
     4681 tcgggtagtg cactggtatc gccttgcaca atagcatatt gaccccacag ccaacaggtt
     4741 ccacccgtag gcatgttgat gatccaatat cctttaggag tatatttccc aacaacatca
     4801 aaatcgctgc ccggttgaac agtcatcaat aactgataga caacatctgg acccgtacgg
     4861 caattggtgg cggtgcttac ggagacagac acgctatctg aagcctcttc agtgggtgct
     4921 tctgtcggtg tggcttcagg cgcttcggta cttacctcgg tcggctcttc ggtggcatcg
     4981 gctgggttgg aggtagtggg caggttacaa gctaaggcgg caatggttaa aacagccacg
     5041 aacataagaa taggttgatt tctcttcatc attattgctc catgaaatgt attcgtctcg
     5101 tattatatga agagtgaaaa agacaaacat cccacagagg gaggatgact tttggattta
     5161 tcacaaatgc ccccaaactc ccattgcaaa cattttgttt gttcgtgacg gaacggtctt
     5221 gttcgtgttt ctttactaag gagaacaaca tgcaaccatc agatatcccc aacgagaaaa
     5281 ccgccaaacg catcggcaac caagccagct ttattattct tgctttggtc ttttcttgcg
     5341 tggtcagttt tttctttctc tttaacgtcg acaagatcaa aatgctacaa acctccatag
     5401 acctgcaatt caatggcagt acaaccactg gaacggttat cagtctggaa gaattcaaaa
     5461 gcagcgcggg tcataacgcc gttttcatct taacagcaga gttcgaagta gatggacagc
     5521 cctacaccgt cacaggcaat gcctactacc caaccctcga ccatagttgg caaggcgaat
     5581 cgttggatgt gatctatgac ccacaagacc caaacattgc catgatcaac acctttgaag
     5641 agcgctgggt cattccactt gcaggggcgt tgccctaggt aaactttatc aatacaggaa
     5701 ccaagatgaa atcagatgtt atcttccccc ttactcgtat tattgccgga atcgttgttt
     5761 tgtttctagt tgccgccttt ggtatcttat tctttttgcc agaccaaacc ggcacattgt
     5821 tcgcatgggc aatcaaacct cacatgtcct ccatgttctt tggttccgct tatttgggcg
     5881 gcgcgtggat tctcgctcaa acggctttcg gtccgcgttg gcatcgggtg caagccgtct
     5941 tcccagcggt aaccatcttc accacagcta tgttggctgc cacgcttccg cattgggagc
     6001 gcttttctct tggcaccatt cccttccttg cctggttgat cttatatatt gtctctccgt
     6061 ttttgattcc tgctctgtgg atatacaacc gca
//
LOCUS       DAOREF010000040         6080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6888_length_6080_cov_3.464896, whole genome shotgun sequence.
ACCESSION   DAOREF010000040 DAOREF010000000
VERSION     DAOREF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6888_length_6080_cov_3.464896"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1638)
                     /locus_tag="PKI33_01425"
     CDS             complement(250..1638)
                     /locus_tag="PKI33_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNM35686.1"
                     /translation="MSNENIQAVENGLLAAVSIHGVPEKKHTLMDRMKYFCVPGLSIA
                     VINNGEIEWARSYGVLEAGKNQAVSSDTLFQSASISKPVSAMMALKLVEQDRLNLDQD
                     VNEILSSWKIPENEFTDTNKVTLRGILSHTAGLTVHGFQGYDFDGEVPSLLQMLDGLP
                     PANSDPIRVDVRPGSVFRYSGGGYTVLQQILEDVSHKTFDELLRNEILDQLNMPDSTF
                     EQPLPAHLRNKAATAHVDLGIPIQGKWHVYPEKAAAGLWSTPTDLAKFAIEIMNSCNN
                     RSNKMLSKNMADSMLTTIQGDYGLGLVVKHLENNIIRFGHSGGNVGFRCYFAAYTGSG
                     KGAVVMTNSDNGNFVAMEIIRSLSRIYEWSDFQPMEKQIDRQVGSDVYSQYVGKYQHP
                     EYSDFFVQIEKDGSGLFIEAIPGAPRWELYPESETRYFCVETETSIEFQKNNDGVFDA
                     LIYFSMELKKVV"
     gene            complement(1855..2478)
                     /locus_tag="PKI33_01430"
     CDS             complement(1855..2478)
                     /locus_tag="PKI33_01430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM35687.1"
                     /translation="MSRFGSDPLSFFNSVYQNIAPWDIGTPQPAMAALIEKHPPANPV
                     LDLGCGSGDLAIYLAKLGNQVVGIDFVESAIKNAQDKVKSLPDETAQIIKFQVADALK
                     PSLLQDKFGAVFDSGFYHLFNPDQCEQLIDEVASILKPHGCYYLHEFAVEFSIPNVPR
                     QISIDELQTRFTVEKGWRIKEVQTVEFLSRVAPPVPAICACVERLPM"
     gene            complement(2570..3721)
                     /gene="chrA"
                     /locus_tag="PKI33_01435"
     CDS             complement(2570..3721)
                     /gene="chrA"
                     /locus_tag="PKI33_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNM35688.1"
                     /translation="MTDITKSDISQESYWQLFLRFLRFGFLAWGGPVAQIAMIRQELV
                     DEEKWISKERFNRVLAVYQVLPGPEAHELCVYFGMIARGRIGGLLAGLGFMLPGFVLM
                     FALSWVYVTYGINSPIFAAVFYGCKAAVVALIIRAIHRIGGHSLTDYWLLGIAIAVGL
                     ASLLGVNFLFSLPLAGFAYSVIKRQSPSSVLHALLPLPLFAQSAGILAPSLFALFGYG
                     LRSGLLTFGGAYTVISFLQYDAVVTGSWMTNAQFLDGLALSGILPAPLIIFATFVGYL
                     GGGAIGAIALTIGIFLPAFGFTLIGHEYVERLVENKSAHAFLDGVTAGVVGLIVVTAI
                     GLFREAVFDIPTIIIFALALLAVYRWKAKAAVVGIMFGAGLLGWLLFGV"
     gene            complement(3908..4837)
                     /locus_tag="PKI33_01440"
     CDS             complement(3908..4837)
                     /locus_tag="PKI33_01440"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNM35689.1"
                     /translation="MQPDNASSPNIYINEDLVGQLIASQFSKWANLPLKPVEVSGWDN
                     RTFRLGNDMSVRLPSEAWYVAQVEKEQYWLPKLAPFLPVQIPIPLALGYPDKNYPWHW
                     SIYKWLEGENASIENIKNLHEFAISLAQFLVALHQIDSSGGPPPGQHNFFRGGALKVY
                     DDETRSSISALRNEINFDVAAEVWEIALDTTWNKEPVWLHGDFASGNLLVNKGQLSAV
                     IDFGCLGIGDPACDLTIAWTFLSGKSRNAFIKTLALDDATWARARGWALWKALITLVE
                     HIEKDDNKSKYARYVINEVLEDHKYRGDTFKRI"
     gene            5122..5853
                     /locus_tag="PKI33_01445"
     CDS             5122..5853
                     /locus_tag="PKI33_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNM35690.1"
                     /translation="MPAKAQTGRPQEHGWRIILNSIFYILQSGCSWRMLPSEMPPWKT
                     VYHYFRLWRKNGTWERINRLLREKVRLKFGKKRQPSACILDSQSAKTSEGGAERGFDA
                     GKKVTGRKRHTLVDTLGLILKVVVTAGNVQDRDGAKVLLEEISTEQDVIKRLELVWAD
                     GGYRGDLLAWVEETFGWKLEIVEKSKDYKGFQVLPKRWIVERTFAWLIRQRRLARDYE
                     RLPETSEAFIYIAMIRLMLRRLASL"
BASE COUNT         1784 a         1409 c         1299 g         1588 t
ORIGIN      
        1 cggtgcacgt gccgctgttc tcgacctcgc gcgccccggt gcgcaagctc taccgtacga
       61 ccgcgccgct cgcgctgttc ggcctttctc tataagagtc tcatttattc catatgggta
      121 cttaatacaa accaccaata gcgagtctta ctggcgggat tagcaaaaag ttacgggtag
      181 aatctgacca agggtacaaa atcctctttc aggaggtaaa gaccgcactg cttcgtcggg
      241 aaattccaat cagacaacct ttttcaactc cattgagaaa taaatcaagg catcaaaaac
      301 gccatcattg tttttctgga actcaatact tgtttcggtc tcaacgcaaa aatacctggt
      361 ttccgattcg ggataaagtt cccagcgtgg cgcacctgga attgcctcaa taaacaagcc
      421 tgatccatcc ttttctattt gaacgaaaaa gtcagaatac tcaggatgtt ggtactttcc
      481 cacgtactga gaataaacat cagaaccaac ctgacggtca atttgttttt ccataggctg
      541 gaaatccgac cactcataga tgcgtgagag gctgcgaata atttccattg ctacaaaatt
      601 gccgttgtca ctatttgtca tcacaacggc acccttgccg ctgcctgtgt aggccgcgaa
      661 gtagcaacgg aaaccaacat tcccaccact atgaccaaaa cgaatgatat tattttccaa
      721 atgctttacc accaaaccca atccataatc tccctggatc gtcgtaagca tgctgtcagc
      781 catatttttt gataacatct tattcgaacg gttgttacaa gaattcatga tctcaatggc
      841 aaattttgcc aggtctgtgg gcgttgacca taaaccggcg gctgcttttt cggggtatac
      901 atgccatttc ccctggatgg gtatgcctaa atcaacatgc gccgttgcag ctttatttcg
      961 caaatgcgct ggaagcggtt gttcaaatgt gctgtctggc atatttaact gatccaatat
     1021 ttcattccgc aacagttcat cgaacgtttt atgagataca tcttcaagaa tttgctgcaa
     1081 gactgtatat ccacccccag agtatctgaa cacgctccca ggtctaacat ccacacgtat
     1141 cggatctgaa ttagccggtg gcagcccatc aagcatttgc aacaatgatg gtacttcacc
     1201 atcaaaatca taaccttgaa agccgtgaac tgttagccca gcagtgtgtg aaagaatgcc
     1261 gcggagagtt accttgtttg tgtctgtaaa ctcattctct gggatcttcc atgaagaaag
     1321 aatttcattt acgtcttgat ctaaattcaa tctgtcctgc tctacaagtt tgagcgccat
     1381 cattgccgaa acaggcttac taattgaggc tgactggaac aatgtatcgg atgaaactgc
     1441 ctgattctta cccgcctcca acacaccata gctgcgcgcc cactcaatct ccccattgtt
     1501 tatcacggca atgcttagac caggaacaca gaaatatttc attctatcca tcagggtgtg
     1561 ttttttttcg ggaaccccat gaatggaaac cgccgccaac aaaccattct caacagcttg
     1621 aatattttca tttgacatgt ctatcacctt ttctattggt tctgcatgct ttgtcttcaa
     1681 acaacgcttt tatttcactc actcatggga attaacttct gcatgaaaat attgtgagag
     1741 tacgcgaagc cgctcaacgg tttgcgttat ctgtgctggg acggagacgg caaagccatc
     1801 caatcaaaaa atgatgcggc atagaagccc cgtgcatgtt ttgtcaggtg tggtttacat
     1861 tggaaggcgc tctacacaag cgcagatcgc tggcacggga ggcgcgaccc gactaaggaa
     1921 ttccaccgtt tgaacttcct taatgcgcca acctttttca acagtgaaac gtgtttgtaa
     1981 ttcgtcaatt gaaatttggc gaggcacatt aggtatagag aattcgactg cgaactcatg
     2041 caaataataa caaccatgcg gtttcaagat tgaggcaact tcatcaatta attgttcgca
     2101 ttggtctgga ttaaataaat gatagaaacc tgaatcaaaa accgctccaa atttatcttg
     2161 aagtaaagat ggctttaggg cgtctgccac ctgaaacttt attatttggg ccgtctcatc
     2221 gggaagggac ttaactttat cctgggcatt cttgatggca gactcaacaa aatcaatccc
     2281 aacaacttga ttacccagtt ttgccaaata aatggcaagg tcaccagagc cgcaaccgag
     2341 atccaaaact gggtttgcgg gcgggtgttt ttcaataaga gcagccattg cgggttgagg
     2401 cgtaccaata tcccaaggcg caatattctg gtaaacagaa ttgaaaaaag aaagtgggtc
     2461 agaaccaaat ctactcaaaa caaatctcct tgtatgtttt cgttaagcgc cttacggcaa
     2521 tccaaagggt cagacgcatg cttcctcggc acacttttga cctcaaaact cacacgccaa
     2581 acaatagcca acccaacaag cctgcaccaa acataatacc tacaactgcg gcttttgctt
     2641 tccaacgata caccgctaat aacgccagcg caaaaataat gattgtaggt atatcaaaaa
     2701 cggcttctcg aaacagtcca attgctgtaa caacgataag ccctactact cctgcggtga
     2761 cgccatctaa aaatgcatgg gcagatttgt tttctacgag cctttcaaca tattcgtgac
     2821 caataagagt aaaaccaaaa gcaggtaaaa atattccaat tgttaacgca attgcgccaa
     2881 ttgcgccgcc gccaagataa ccgacaaaag tagcgaagat aataagcgga gcgggaagaa
     2941 tgcccgacaa cgccaatcca tctaaaaatt gagcgttggt catccagctg ccagttacaa
     3001 ctgcgtcata ttgcaagaac gaaatgactg tgtatgcacc gccgaaagtc aataaaccgc
     3061 ttcggaggcc ataaccgaac aatgcgaata gtgaaggggc gagtattcca gcactttggg
     3121 cgaataatgg tagaggtaat aaagcatgta aaacagatga cggcgattga cgcttgatga
     3181 ccgaataagc aaacccagcc agaggaaggc tgaaaaggaa attgacgccc agcaaactgg
     3241 caagtcctac ggcaatggca atacccaaaa gccaatagtc agttaaagaa tgtccaccga
     3301 tgcgatgaat ggcgcgaatg atgagggcta caacagcggc tttacatcca taaaatacgg
     3361 cggcgaaaat aggtgagtta atgccgtaag taacatacac ccaggacagt gcaaacatca
     3421 acacaaagcc ggggagcata aacccaagcc ctgccaacaa accgccaata cgtccccttg
     3481 ctatcatacc gaaataaaca caaagttcat gggcttcggg tccaggcaaa acttgataaa
     3541 cggctaaaac acgattgaat ctttcttttg aaatccactt ttcttcatca actaattcct
     3601 ggcgaatcat agcaatttgg gcgacgggac caccccaagc caaaaaaccg aagcgcagaa
     3661 aacggaggaa aagttgccag taagattctt gcgaaatgtc agatttggtt atgtcagtca
     3721 ttgagtatcc tattttaaca ataaaatgag attgttatgc aagggcgtac caacggcata
     3781 cgttacctgc gctggggcgg agacggaaaa gccgtccagc cagaaagctg cttgggatat
     3841 gcacatgcct atgggaagtc cctagcatca ggtaaatact ttattaggcg ttttttattt
     3901 tttagactca tatccttttg aaggtatcac ctctgtattt gtggtcctca agcacttcat
     3961 tgatcacata tctggcatat tttgatttat tatcatcttt ttcaatatgt tcaacaagtg
     4021 taattaacgc cttccaaagt gcccaaccac gagcacgcgc ccaggttgcg tcatctagag
     4081 caagagtttt gataaacgca tttcgacttt ttcctgacaa aaacgtccat gcgatagtca
     4141 aatcacatgc tggatcgcca attcccaaac atccaaaatc tataactgcg cttaactgtc
     4201 ctttatttac cagtaaattt cctgatgcaa aatcaccatg taaccaaacg ggttccttat
     4261 tccatgttgt gtcaagggct atttcccaaa cttcggcagc cacatcgaag tttatttcat
     4321 ttcttaaagc ggaaattgaa ctgcgagttt catcatcata caccttgagt gctccaccac
     4381 gaaaaaagtt atgttgtcca ggcggcggac cgccagacga atcaatttga tgaagtgcta
     4441 caaggaattg agcaagtgaa attgcaaact catgaagatt tttaatattt tcaatcgaag
     4501 cattctcgcc ttcaagccat ttatagattg accagtgcca cggataattt ttatcaggat
     4561 agccaagtgc caatggaata gggatttgaa ctgggagaaa aggtgcaagt tttggcaacc
     4621 aatattgttc tttttccact tgcgcaacat accatgcttc actgggaagc cttacactca
     4681 tatcgttacc aagacgaaag gttcgattat cccatccact aacctcaact ggcttaagtg
     4741 gaaggtttgc ccattttgaa aactgcgatg caatcaattg acctacaagg tcttcgttaa
     4801 tataaatatt aggagaagat gcattgtcag gctgcatatg tactccatat cattttggaa
     4861 ggaaatgtct aacgggttgc gctacttggt tggcgaatcc gtccgactgg aaaaatgctt
     4921 gaggtgtgtg cacgccttac gtcagtcccc agcgtcatat ccgtgctttt ttaggtggcg
     4981 acccattacc attatttctt agagagtgtc tgaaaacttg aaaaaatagg agtggttggg
     5041 caaacttgct ggatgaccaa atcacaacaa acttacccaa ccgacctgaa tgatacacaa
     5101 tggttgaaaa tttgaccatg tctgccagcc aaagcccaaa caggtcgtcc gcaagaacat
     5161 ggctggcgca ttatcttgaa cagcattttt tacatcctac aaagcggttg ttcctggcga
     5221 atgttaccga gcgaaatgcc accgtggaag actgtctacc attattttcg tttgtggcga
     5281 aaaaatggga catgggaacg aatcaatcgc ctgctccgtg aaaaagtgcg gttgaagttt
     5341 gggaaaaagc gtcaacccag tgcttgtatt ttggatagtc agtctgccaa gacctcagaa
     5401 ggcggtgcgg agcgtggttt tgatgctgga aagaaggtga cgggtcgaaa aaggcatacc
     5461 ttggtagaca ctttgggctt gatcctcaaa gtcgtggtga ctgctggaaa tgtccaagat
     5521 cgagacggtg caaaagtctt gttggaagaa attagcacag aacaagatgt catcaagaga
     5581 ttggaactcg tctgggctga tggtggttat cgcggtgatc tactcgcttg ggttgaagaa
     5641 acctttggtt ggaaactgga gattgttgaa aagtccaaag actataaggg gtttcaagtt
     5701 cttcccaaac gttggattgt ggagcgaact tttgcttggt tgattcgcca acgcagactt
     5761 gccagagatt atgaacgatt acctgaaaca agtgaggctt tcatctacat tgccatgatt
     5821 aggcttatgc ttcgcagatt ggcttcacta tgacttttca gacactctct tagccccctc
     5881 tgtccgttgg acatctcccc caaatacgac ggtagtgctg tcggatttgg gggagaacta
     5941 gaaattgaaa ttttttatga acactgagag tatttctaaa actttgattt cagacgcaac
     6001 cgtccctccc cgatttcgac gtgctctccg tcgcaattgg ggaggttagg aggggacaaa
     6061 atgccgcgtc cacccagaag
//
LOCUS       DAOREF010000041         6077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6894_length_6077_cov_5.057954, whole genome shotgun sequence.
ACCESSION   DAOREF010000041 DAOREF010000000
VERSION     DAOREF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_6077_cov_5.057954"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PKI33_01450"
     CDS             <1..1307
                     /locus_tag="PKI33_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM35691.1"
                     /translation="HMNETLNIGGALLVKLFGRTREEEKRFRERAANVRDIGIRRAVI
                     GSTFFVIFGLVTAVGTALVYGLGGYYVITDVFTVGTIVAFGSYLGQLYGTLQGLASAP
                     VDFSTSMVSFERVFEIIDLPQDIVEKENAVELKDVKGELQFDNVTFNYKVDESILLKD
                     VKRYGSMDNVKAVISGGNGDASVIKRRVERAASDSAEADSTSQARDIALEGISFTARP
                     GNLVALVGPSGAGKTTVTYLIPRLYDPSDGVIRIDGHNLKDVTLESLSQSIGMVTQET
                     YLFHDTIRTNLTYAKPDATQEEIESAAKAANIHNFIMDLSDGYDTIVGERGYRLSGGE
                     KQRIALARVILKNPRILVLDEATSSLDSESEALIQEALKRVMAGRTSIVIAHRLSTIL
                     AADMILVMDKGKIAERGTHEELLATGGIYSQLYETQFRAERV"
     gene            complement(1355..1810)
                     /locus_tag="PKI33_01455"
     CDS             complement(1355..1810)
                     /locus_tag="PKI33_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNM35692.1"
                     /translation="MKAYDVVNQHLRAVEAGDWEKADSFIADDYSMSGTIPFPVSLFI
                     KIGKKESLRMHKPRKTALPDFKFNEKILEETDEMVSFQVNLTGTHTGVIDYTGILRGV
                     PVIQPTGKKVELPDEFFTYFVRAGKIYKTIGRIPKNAGVQALVRAVTGS"
     gene            complement(1873..2277)
                     /locus_tag="PKI33_01460"
     CDS             complement(1873..2277)
                     /locus_tag="PKI33_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35693.1"
                     /translation="MKYLIYVFYFEILINFASGGQSLFMPAAFLGQFSSEPAPAFALE
                     MTRWYGVLIAVLTYLLIRGLMLRGAALKVALEALLFGDVIQIIVSFVTANALGGWTSN
                     VILSVVVSVILGVVRLICLWKPVETGIERSTP"
     gene            2423..5125
                     /gene="ppc"
                     /locus_tag="PKI33_01465"
     CDS             2423..5125
                     /gene="ppc"
                     /locus_tag="PKI33_01465"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNM35694.1"
                     /translation="MEISQNIRLLGNLLGKVISEMESPELFDIEERIRALAKARRNGD
                     ADAALKLQQAVSGLKDADARVVAAAFTAYFDLVNLAEEDHRVQMLRKQEQDSFPAPAH
                     ESIGEAIEVLKRNGMTSDEMNALMQSLSIELVLTAHPTEARRRTILSKTERIAAQLDL
                     IARNGLYSYEKQNAHNALSAAILALWLTDRTRADKLTVTDEVRTGLYFVESYFWNTLP
                     ALYHDLELALATHYPDVKPPHHWLKLASWIGGDRDGNPFVKTDITAETLRLHRGLAVE
                     GLRREFQELGRQLSVSSSRILPPPELLEWIEKRRPFPKRVEYIEKRYANEPYRLAIAL
                     LSNDLAEASKEEMTTHLLGTHEHQARLDIREVLHVLSLIASVLPESLVQDQLQAVIHK
                     AQIFGLRAARLDIREDSSRFNSALSEVLRALNIEPNFESLPDDARTELLERLLRDPVP
                     SLAPSLGVTAAAAETWALFKLIGRTNEIYGSDLLGPIVISMTHAAADVLTVLLLAKWG
                     GCKTLPSITPLFEVIQDLKDAPIILEQLFTSDYYREHLQSHNHEQMVMIGYSDSNKDG
                     GYVMANWSLYQAQEEITRVAAKHNVKLTIFHGRGGTIARGGGPANNAIRAQPAGSING
                     KFRVTEQGEIIASRYANPGLAHRHLEQIASAVILASAPHKEEPIPAKWRETMETISNA
                     GFRAYRSLVYETPGFIEFWEAATPLDEIKRLQIGSRPASRAKAGAVNQIRAIPWVFSW
                     MQSRFNLPSWYSLGSGLEAVADLSLLQEMYDGWLFFRTLLNNSEISLLKADMDISALY
                     VSLVPDQKLANDVFKTIRTEYERTCGAVLRISRHQNLLELEPLTQQAVQLRNPYVDPL
                     NYIQVETLQRLRKLDDQDGDEAKSLREVMALTINGIAAGLRNTG"
     gene            5180..5752
                     /locus_tag="PKI33_01470"
     CDS             5180..5752
                     /locus_tag="PKI33_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNM35695.1"
                     /translation="MVKIENSKRTILNLGETLLQDKGFNGFSYANIAAELGVKNAAIH
                     YHYPTKEDLGIAVMQRYRERFKLWINNARVKDLTPEGKLDWFLSIYTNMRADQGKICL
                     VGSMEAEFNSIPEGLQSEVQGLHKELLIWLQSTLTEGRDAGAFAFKGEPADKAAMLLS
                     TLQGALQMARALGAKKFHAVIEQIKLDLLK"
     gene            5806..>6077
                     /locus_tag="PKI33_01475"
     CDS             5806..>6077
                     /locus_tag="PKI33_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35696.1"
                     /translation="MNALEPKVDSQAVIKKVYRAFFGPSKYEVKSSDEAILARGKNYR
                     IPFEGGELAVTTWGESGPSILLMHGWGGARAQMTGFVEPLLAAGYRV"
BASE COUNT         1564 a         1497 c         1481 g         1535 t
ORIGIN      
        1 cgcacatgaa cgagacgctc aacatcggcg gcgcattgct cgtcaaactt tttggtcgta
       61 cccgtgaaga agaaaaacgt ttccgcgaac gcgctgcgaa cgtccgcgat attggcattc
      121 ggcgcgccgt cattggctcg acctttttcg ttatctttgg tttggtcaca gctgtgggca
      181 cagcgcttgt ttatggtctc ggcggttact atgtcatcac cgatgttttc accgtcggta
      241 ccatcgtcgc ttttggctcg tatctcggtc aactctatgg aactctgcaa ggtctcgcca
      301 gcgcgccggt tgatttttcc accagcatgg tttcgttcga gcgcgtcttc gagatcattg
      361 acctgccgca agatatcgtt gaaaaagaaa atgccgtcga actcaaagac gtgaagggcg
      421 aactgcaatt cgataacgtc actttcaatt acaaggtgga cgaatcgatc cttctcaaag
      481 atgtcaaacg ctatggcagc atggataatg tcaaagctgt tatcagtgga ggaaatggcg
      541 acgcatcagt cattaagcgt cgggtagaaa gagccgcctc cgactccgcc gaagcggact
      601 caacctctca agcccgcgat atcgccctcg aaggaatctc ctttacggcc cgtcccggaa
      661 atttagttgc cctcgtcggt ccttctggcg ctggtaaaac cacagtgacg tatcttatcc
      721 cgcgcctcta cgaccccagt gacggtgtga ttcgcattga tggtcacaac ctcaaagatg
      781 tcacgcttga gtcgttgtct caatcgattg gcatggtcac acaagagacc tatcttttcc
      841 acgataccat ccgcaccaac ttgacgtacg ccaagcccga tgccactcaa gaagaaattg
      901 agtccgcggc aaaggctgcc aacatccaca acttcatcat ggacttgtct gatggctacg
      961 acaccatcgt cggcgaacgt ggatacagac tcagcggcgg cgaaaagcaa cgcattgctc
     1021 tggcgcgtgt gatcctaaag aatccgcgca tcctcgtttt ggatgaagcc accagctcgt
     1081 tggacagcga atcagaagcg ttaatccaag aggcgttgaa gcgcgtcatg gcagggcgca
     1141 caagcatcgt cattgcccat cgcttatcca ctattcttgc ggcggatatg atccttgtga
     1201 tggacaaagg caaaattgct gaacgcggca cccacgaaga attacttgca acgggtggaa
     1261 tctatagtca gctatacgag acgcagttcc gagcggaacg ggtgtagctt tttcaagaaa
     1321 aaatcccaaa accatggttt tgggattttt ttaattatga gcctgtcact gcccgtacca
     1381 gagcttgaac tcctgcgttc tttggaattc tcccaatggt tttatatatc tttccggcgc
     1441 gaacgaagta tgtaaagaat tcatctggta gctctacttt tttgccggtg ggttgaatca
     1501 cgggcacacc gcgtaaaatg ccggtgtaat cgatcacgcc ggtatgcgtt ccagtgaggt
     1561 tgacctgaaa acttaccatt tcgtcggtct cttccagaat cttctcgttg aatttgaaat
     1621 caggcagcgc ggttttacgc ggtttgtgca tgcgcaggga ttcttttttg ccgatcttaa
     1681 tgaacaaact gacggggaat ggaatggttc cgctcatcga ataatcgtct gcgatgaacg
     1741 agtcggcttt ttcccaatcg cctgcctcca cagctcttaa gtgctggttg actacatcat
     1801 aagctttcat tgatctctcc tttattctaa agacacaata ctgattgatc tctgaaagat
     1861 tttcttacca agctatggtg ttgatctttc aatgccagtt tcaactggct tccacaaaca
     1921 gattaaacgc actacaccca aaataacgga aacaaccacc gataagatca catttgaggt
     1981 ccagccgcca agtgcgttgg cagtgacaaa cgaaacgatg atttgaatca catcgccgaa
     2041 gagtagggct tcgagagcca cttttagcgc tgccccgcgc agcatcaacc cgcggatgag
     2101 gaggtaggtt aacaccgcaa taagcacacc ataccaacgc gtcatttcga gggcaaaggc
     2161 gggcgcaggc tcgctggaga attgaccgag aaaggcggca ggcataaaaa gggattgtcc
     2221 gccactggcg aagttgataa ggatttcaaa gtaaaacaca tagataaggt atttcatggt
     2281 cggctcctga gtgggtgagt atttgaatgc gattatataa ctgttgcagc ttggtacatc
     2341 ttgaaaatga cttgttgcta tcacaaagaa ttccccaaat gggtattgtg agcatacctc
     2401 acttgctata caatcaaaag ttatggaaat ttctcaaaac attcgcttgt tgggtaattt
     2461 gctcggtaaa gttatttcag aaatggagtc gccggaactt ttcgatattg aagagcgcat
     2521 ccgtgcactt gccaaagcgc gtcgaaatgg cgatgcagac gccgccctga aattacagca
     2581 agccgtttca ggcttgaaag atgccgatgc acgcgtagtg gcggcagcct tcactgctta
     2641 ctttgatctc gtgaacttgg cagaagaaga ccatcgtgtg cagatgcttc gcaagcagga
     2701 acaggattct ttccccgcac ccgctcacga gtccattggc gaagcgattg aggtcctcaa
     2761 gcgaaacggc atgacctccg atgagatgaa tgctctcatg cagagtcttt ccatcgagct
     2821 ggttcttact gcacacccta ctgaagctcg ccgtcgtacc atcctctcaa agacagagcg
     2881 tatcgcagcc cagctagatt tgatcgccag aaatgggttg tattcgtatg aaaaacaaaa
     2941 tgctcataac gcgctgtctg ctgccatcct tgcactctgg cttacagacc gcacccgcgc
     3001 cgataaatta accgtcacag atgaagtgcg cactggtttg tactttgttg aatcgtattt
     3061 ttggaacacc ttgccggctc tttatcatga ccttgaactt gcactggcaa ctcattaccc
     3121 cgatgtaaag ccgccacacc attggctaaa actggcttcg tggatcggcg gcgaccgcga
     3181 cggaaatccc ttcgtcaaga ccgatattac tgccgagacc ttgcgcctgc accgcggttt
     3241 ggcagtggag gggttgcgac gtgaatttca agaattgggc aggcagttaa gcgtcagctc
     3301 cagccgtatt ttgccgccgc ctgaattgtt ggaatggatc gaaaagcgtc gcccatttcc
     3361 gaaacgtgtg gaatacatcg agaagcgata tgcaaatgaa ccctatcgcc ttgcgattgc
     3421 tctgttatcc aacgaccttg ctgaagcctc aaaagaagaa atgacaactc atttgctggg
     3481 cacgcatgag catcaagcgc gcttggatat tcgcgaggtc ttgcacgttt tatctttgat
     3541 cgcatccgtc ttgccagaaa gtttggtgca agaccaattg caagccgtca ttcacaaagc
     3601 ccagatcttt ggtctgcgcg ccgctcgttt ggatattcgc gaagactcaa gccgtttcaa
     3661 ttcagcattg agcgaagtct tgcgggcgct gaacattgaa cctaattttg agtcattgcc
     3721 agatgatgca cgcacagaat tattagagcg cctcttacgg gacccggttc cgagtctcgc
     3781 cccgagcctt ggcgtcactg ctgccgctgc tgaaacatgg gcactcttca aactgatcgg
     3841 tcgtaccaat gaaatttatg gcagtgactt gttaggaccc atcgtcattt ccatgactca
     3901 tgctgctgcc gatgtgttga ccgtgctttt gcttgccaaa tggggcggct gtaaaacatt
     3961 accgtctatt acaccgcttt tcgaagtgat tcaagacctc aaagatgctc caatcatcct
     4021 tgagcagctt ttcacgtcgg actattatcg agaacatttg cagtctcata accacgaaca
     4081 gatggtcatg atcggttatt ccgatagcaa caaagacggt ggttatgtca tggcgaactg
     4141 gtctctgtat caagcgcaag aagagattac ccgtgttgca gccaaacata acgtcaagct
     4201 gaccatcttc cacgggcgcg gtggcaccat tgcccgcggt ggcggacctg ccaacaatgc
     4261 catccgtgct cagcctgcgg ggagcatcaa tggaaaattc cgtgttacag agcaaggcga
     4321 gatcatcgct tcgcgctatg ccaaccctgg tttggcacat cgtcacttgg agcagatcgc
     4381 cagcgcggta atccttgcat ctgctcctca taaggaagaa cccattcctg ccaaatggcg
     4441 cgaaaccatg gagaccattt cgaatgcggg tttccgggca tatcgctccc ttgtgtatga
     4501 aaccccaggc tttatcgagt tttgggaagc agcaactcct ttggatgaaa tcaagcgttt
     4561 gcaaattggg tcacgcccgg catctcgtgc caaggcaggt gctgtgaatc agattcgagc
     4621 gattccttgg gttttctcat ggatgcagag tagattcaac ttaccgagct ggtacagcct
     4681 cgggtccggt ttggaggcgg tggctgatct ttcactcctt caagaaatgt atgatggctg
     4741 gctattcttc cgcaccttgt tgaataattc cgagatttca ttactcaaag cagacatgga
     4801 tatttctgct ttatacgtca gccttgtgcc cgatcaaaaa cttgcaaacg atgttttcaa
     4861 aaccattcgc actgaatacg aacggacgtg cggtgctgta ttgcgaatca gccgccatca
     4921 aaacttgctg gaattggagc cgctcacgca acaagctgtg caacttcgca acccatatgt
     4981 ggacccgttg aactacatcc aggtagaaac cttgcagcgc ttgcgaaaac tggatgatca
     5041 ggatggagac gaggcaaagt ctttgcgtga agtcatggca ttaacgatca atggcattgc
     5101 tgcaggcttg aggaataccg gataacctat acaggggaag gcttgacaac ttactaacta
     5161 gtaagtaaaa tttttaccaa tggtcaagat cgagaactcc aaacgcacaa ttctgaacct
     5221 gggcgaaacc ttattgcaag acaaaggttt caatgggttt agttatgcca acatcgctgc
     5281 agaattgggt gtgaaaaatg ctgctattca ttatcactac cctaccaaag aagatttggg
     5341 gattgcggtt atgcagcgtt atcgcgagcg tttcaagtta tggatcaata acgcccgcgt
     5401 caaagacctg acgccagagg ggaaactgga ttggttctta agtatttaca caaacatgcg
     5461 tgcagaccaa ggcaagattt gccttgtcgg ttccatggaa gcagaattca attccattcc
     5521 cgaagggtta caaagcgaag ttcaaggctt acataaagaa ttgctaatat ggcttcagtc
     5581 cactttgact gaaggcagag acgctggcgc ttttgcattc aaaggagaac ctgcagataa
     5641 agccgcaatg cttttatcga ccctgcaagg cgcattacaa atggcgcgtg ccttgggcgc
     5701 taagaaattc cacgctgtga tcgaacagat taagttagat ttactcaaat aaattttttt
     5761 tgtcaataaa acttactagg tagtaagttt tcaaaaggag aaactatgaa tgctttagaa
     5821 ccaaaagttg attcgcaagc tgtaataaag aaggtgtatc gcgccttttt cggaccgagc
     5881 aaatatgagg tgaaatcatc ggatgaagct attcttgctc gaggaaagaa ttatcgcatc
     5941 cccttcgagg gtggcgagct ggctgtcacc acatggggcg aaagcggacc atccatctta
     6001 ttaatgcatg gctggggtgg tgctcgtgcc caaatgaccg gtttcgttga gccgcttctt
     6061 gcggcagggt atcgagt
//
LOCUS       DAOREF010000042         6034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_6983_length_6034_cov_3.159893, whole genome shotgun sequence.
ACCESSION   DAOREF010000042 DAOREF010000000
VERSION     DAOREF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6983_length_6034_cov_3.159893"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="PKI33_01480"
     CDS             complement(<1..1555)
                     /locus_tag="PKI33_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNM35697.1"
                     /translation="MTDVFISYSRRDKVFTQKLVDALQTANREVWADWASIPAASDWD
                     AEIKEGIEKTNTVLFVLSPEWVKSNECRKELEHALKMGKKLLPILYIMPEPGQEVPPE
                     LAKINWIYMREADNFEKAFETLQSAMDTDLDWTKLHTRIQVRAVEWSKRNKDNSLTLR
                     GNDLSEGEQFISQGAQKTPEPTPLQGEYILLSRKEATRRQRMTLAGVTVALVVSVALG
                     IVAFFQRQIAVRAKAEAVLNAEVSFARELAALAKNNINVDPEFSTLLSLEAINTLKNV
                     DQPVLQSVEEALREAVQANRLRYINENKYRDDSIYSSAISPDGSVLATGSWGGKVILW
                     KFTEATFPVESTGYSTSTVTLEPLFQIDEQFDIVYTIAFSPDGKKIATSSSNSAIEPA
                     VWDAKTGKKLFSLSGHNQFIRSITFSPNGKLIATASEDGTIRIWNANTGDELLIIKDH
                     IHEVVEYANFNSVYDVAFSPDSSAVVSGGGDRFVLISDTNTGRVIKSYAGEGIILSVA
                     FNPDGSAIAW"
     gene            1678..2319
                     /locus_tag="PKI33_01485"
     CDS             1678..2319
                     /locus_tag="PKI33_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNM35698.1"
                     /translation="MSRNYVVPLTEIRREQMVVNSRFIATLAPAFSIDEARTFMKRIR
                     EEFADASHNVPVYVIGGGNTVTEYFSDDGEPSGTSGKPALTVLRGSGLGDAVLVVTRY
                     FGGTLLGTGGLVKAYTEAAQSVVNVVERGRRVPVHVAMAAIPYNLLERVRLLTTKNSG
                     SILGEEYASDITLTLQFPVEYYTGFQTDLRELTAGKIQAEVIETKEEIIRVDK"
     gene            2381..4579
                     /locus_tag="PKI33_01490"
     CDS             2381..4579
                     /locus_tag="PKI33_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNM35699.1"
                     /translation="MKDKNITIPTIETNRELQGEFFRAVILSIMALTGVFLVFAILIF
                     HELLLRTLIISGGVFFLSVFILFLIKRKKVFLASVFFVGFLWLLVASLTYLTGGVTAP
                     IFAGFLIVILFSGLIFNFRIGVSVALWTVLFGSVLIFAHSKGFLPLAVDYSNGARLAI
                     YTFFFFIALYIQKAAIDSTKLATEKAYISENQYQLFLENIPTITYINDISPEARTLYV
                     SPQVENLLGYSPKTFLQDPALWMKIINEDDVENVLLKNQASVETGEPFKVEYRLMTKS
                     GNVVWVRDEAKLVHDDHGEPAYWLGVWTDITKEKSSEKTLAYMVQTQSMRNNQLQTAS
                     EVSRAATSILELSELMPKVVELIRSHFDYYYVGIFLVEKVTNNAILAAATGSAGKEML
                     AKGHSLPIANSSMIGWSILNNRARIALDVGKDAVRFKNPLLPLTRSEVAFPLRFHGRV
                     IGAMTIQSALPSAFTDADITALQTMADQVANAIETARLLDEREALIDELETKNAELER
                     YTYTVSHDLKSPLVTIRGYVGYLKESAVKGDMQRFEVDMKRVIKATETMQALLNDLLE
                     LSRVGRVINSLTNVPFEDMVKDVLTLVVEPQKGQTEWFVVQKGLPTVRCDRTRILEVL
                     QNLILNSIKFIGDQPKPSIRIGQAGFDNKTNFPIFYVADNGIGIEPKYHETVFGLFNR
                     LDQKEEGTGIGLTLVKRIIEVHGGRIWLESEGRGKGTTFYFTLPPAEMKP"
     gene            4694..5185
                     /locus_tag="PKI33_01495"
     CDS             4694..5185
                     /locus_tag="PKI33_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNM35700.1"
                     /translation="MSNSNRTKLLLDIGTFIAFLITMDPRFSGLAIHEWLSLALAGTI
                     IVHLLLNWDWIVNVTRRLFGKANANARVNYVLNWLLFIDGILIMLSGIMISEVAIPSL
                     GLALPMNFAWRRLHDMSANLALLIMGLHLAMHWNWIVTTFKRVFLGGFKKKQPVIAAI
                     EEA"
     gene            5188..5502
                     /locus_tag="PKI33_01500"
     CDS             5188..5502
                     /locus_tag="PKI33_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35701.1"
                     /translation="MNTLFRVLIILVIATIIGGLMYVGVSTSNSSTNGFPEFEGGRER
                     PPFAEGSEFRPDGDNDGERGERGGFGFPGGIIKAVVLMSVAGGIYSGIVWAGKKTKQT
                     LA"
     gene            complement(5545..>6034)
                     /locus_tag="PKI33_01505"
     CDS             complement(5545..>6034)
                     /locus_tag="PKI33_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM35702.1"
                     /translation="LADAGELSVDFQPIDINNFMSDIHAHYLTLFNQKRISLDLETAS
                     TTLTANLDPNRFAQVLKNILDNALRHTPEGGRVELKTQLTGSSIQLSVKDSGDGVHPE
                     EATHLFDRFYRVDESRTRDDGGSGLGLAIAKSIVEMHKGRIWAESEKGKGLKVIIEIP
                     KI"
BASE COUNT         1610 a         1207 c         1380 g         1837 t
ORIGIN      
        1 cccaggcaat tgcagagcca tctggattaa aagcaacaga aaggattatc ccttctcctg
       61 cataactttt tataacccta ccagtgtttg tatcagagat taatacgaat ctatctccac
      121 cgccgcttac tacagcactg ctgtcagggc taaatgcaac atcataaaca gagttaaagt
      181 ttgcatactc aactacttca tgaatatgat ctttgataat aagcaattca tcgcctgtgt
      241 ttgcattcca tattcttatt gttccatctt cgcttgcggt agcgattagt ttgccattag
      301 gactgaacgt aatagaccga ataaactgat tatgaccaga tagagagaac agctttttgc
      361 cggtttttgc gtcccaaaca gctggctcaa ttgcagagtt ggaagaagag gttgcaatct
      421 ttttcccatc cgggctaaaa gcaatagtat atacaatatc aaattgttca tcgatttgga
      481 ataatggctc cagagttaca gtgcttgtgg aatagcctgt actctctacg gggaacgtgg
      541 cttctgtaaa cttccaaaga ataactttcc caccccatga ccctgttgca aggactgagc
      601 catctggact aattgctgat gaataaatac tatcgtctct gtatttattt tcatttatat
      661 aacgaagtct gttggcttga accgcttctc gaagtgcctc ttctacgctt tgtaaaacag
      721 gctggtcaac attttttaga gtgttaattg cctctaacga tagaagagta ctaaattcag
      781 gatcgacatt tatattgttt tttgccaatg cggcaagttc tcgagcaaat gacacttccg
      841 cattaagaac ggcttcggct ttcgccctga ccgctatctg tctttgaaaa aacgctacaa
      901 tgcctaaggc aacagagaca accaatgcca cggtcacgcc tgctaatgtc attcgctgtc
      961 tgcgggtagc ttcttttcga cttaaaagaa tatattcgcc ttgtaatggg gtcggttctg
     1021 gggtcttttg cgcaccctgc gagataaatt gctcaccttc agagaggtca tttcctcgca
     1081 aggtcaagga gttatcttta tttcttttac tccactccac agcgcggact tggatacgag
     1141 tatgtagttt tgtccaatct aagtcggtat ccatggcact ttgcaaagtc tcaaatgcct
     1201 tttcaaagtt gtccgcttca cgcatatata tccagtttat ttttgcaagt tctggtggaa
     1261 cttcttgccc tggctcaggc ataatgtata gaataggcag caattttttc cccattttta
     1321 gggcatgttc caattcttta cggcattcat tggatttgac ccactcaggg cttagaacga
     1381 ataagaccgt gttggtcttt tcaatccctt ctttgatctc tgcatcccaa tctgaggcgg
     1441 ctggaatgga agcccaatca gcccaaactt cgcggttggc ggtttgaagt gcatctacca
     1501 gtttttgggt aaagacttta tcacgacgag aataagaaat gaagacatca gtcatagcag
     1561 tttccttaaa tcagggaatg aatttataaa aagattttac tgatttaata aagttgtaca
     1621 tttacaatat ggtaagcttg cagctatgaa aataagattt ttttgtccga ttaaattatg
     1681 tctagaaatt atgtagtgcc tttaactgaa attcgccgtg agcagatggt ggtcaattcg
     1741 cgcttcattg ctacactcgc gcctgctttc tccatcgatg aagctcgtac cttcatgaag
     1801 cggattcgag aggagttcgc agacgcgtct cataacgtcc ctgtttatgt gattggtggt
     1861 ggaaatacgg taacggaata tttttcagat gatggagagc caagtggtac ctctggcaaa
     1921 cctgcgttga ctgttttgcg tggcagcgga ttgggagatg cggttttagt agtcacccgt
     1981 tattttgggg gcaccctgct tggaactggc gggctggtca aggcgtatac agaggcggcg
     2041 cagtctgttg tcaatgttgt agaacgcgga aggcgagtcc ctgtgcatgt ggcgatggca
     2101 gcgattccct ataacttgtt ggaaagagtt agattattga ccaccaagaa tagcggcagc
     2161 atcttaggtg aagagtatgc ctcagatatc acgttgactt tgcagttccc cgtagaatat
     2221 tacactggct ttcaaacaga tctgcgagaa cttacggctg ggaaaattca agcagaagtc
     2281 attgaaacaa aagaggaaat catacgtgta gacaagtaag tttttgagtt ttactccctc
     2341 aataaaaaaa catgtataaa gtaaaattca aagtattgtt atgaaagaca aaaatattac
     2401 tattccgacc attgagacta atagggaact gcaaggggag ttttttcgtg ccgttatttt
     2461 atctataatg gctttgacgg gtgtttttct ggtttttgct attcttattt ttcatgaatt
     2521 gttgttgcgg acacttatta ttagtggtgg ggtatttttt ctttctgttt ttattctctt
     2581 cttgattaaa agaaaaaaag tatttcttgc gagtgttttt tttgtagggt ttctttggct
     2641 tttggttgca tctctgacct atcttactgg cggagttact gctcctattt ttgctggttt
     2701 tttaatcgtt attcttttta gcgggttgat ttttaacttc cgtatagggg tttccgttgc
     2761 tttatggact gttctttttg gtagtgttct gatttttgca cactcaaaag ggtttttacc
     2821 acttgccgtg gactattcaa atggcgctcg attggcgatt tatacatttt tcttttttat
     2881 tgcgctttat attcaaaagg cagcaattga cagcacaaag ctagcaacag aaaaagctta
     2941 tattagcgaa aatcaatatc agctctttct tgaaaatatc cccacgatca catacatcaa
     3001 tgatatatcg ccagaagcgc gcacactata tgtcagcccg caggttgaaa acttgcttgg
     3061 gtattctcca aaaacctttc ttcaagatcc agcactttgg atgaaaatca ttaatgaaga
     3121 tgatgttgaa aatgttcttt tgaagaatca agcttctgtt gaaacaggtg agccgtttaa
     3181 agtggaatat cgtcttatga caaaaagcgg gaatgtggtt tgggtgcgag atgaagccaa
     3241 actggttcat gatgaccatg gagaaccagc ctattggttg ggggtgtgga ccgatattac
     3301 caaggaaaaa agttcagaga aaacattggc gtatatggtt caaacgcaat ccatgcggaa
     3361 caaccagttg cagacggcga gtgaagtgtc acgagcggct acctctattt tagagttgag
     3421 cgaattgatg cccaaagttg tggagctaat ccgctcgcat tttgattact actatgtggg
     3481 tatcttttta gttgaaaaag tgacgaacaa tgccatactt gctgcggcaa caggttcagc
     3541 gggaaaagaa atgcttgcga aaggacattc tttgcccatc gcgaactcat ccatgattgg
     3601 ttggtcgatt ctcaataatc gggcgcggat cgctttggat gttggtaaag atgcggttcg
     3661 tttcaaaaat ccgcttttgc cacttacacg ttcagaggtg gctttcccgt tgaggtttca
     3721 tggaagagtc attggagcga tgacgattca atctgcatta ccatctgcgt ttacagatgc
     3781 tgatatcacc gcactacaaa ctatggcaga tcaggtagca aatgcaattg agactgcacg
     3841 tttattagac gagcgcgaag ctctcattga tgagttggaa acaaaaaatg cggaactaga
     3901 acgatacact tataccgtat cgcatgattt gaaatctcca cttgtaacga tccgcggcta
     3961 tgtggggtat ttaaaagagt ctgctgttaa gggagatatg cagcgttttg aagtggatat
     4021 gaagcgcgtc atcaaagcga ccgagacaat gcaagcactg ctcaacgact tattggaact
     4081 ttccagggtt gggcgtgtaa ttaattctct aacgaatgtc ccatttgaag atatggtgaa
     4141 ggatgttttg accctggtcg ttgagccgca aaaaggacaa acagagtggt ttgttgtgca
     4201 aaaagggctt cctacagtgc gctgtgaccg gaccagaatt ttagaagtgt tacaaaacct
     4261 gatcttgaat tccataaaat ttatcggcga tcaacctaaa ccctccattc gcattgggca
     4321 agcgggcttt gataataaaa cgaactttcc tattttttat gtcgctgata atggaattgg
     4381 catcgaaccc aaataccatg aaacggtttt cgggttgttc aatcgactgg accaaaaaga
     4441 agaggggaca gggattgggc ttactttagt aaagcgcatt attgaggtcc acggcgggcg
     4501 gatttggctt gaatcagaag ggcgtgggaa aggtactact ttttacttta ccctgcctcc
     4561 cgctgaaatg aaaccctagt tttacaactt cttcataatc ttttcgcatt taatccacct
     4621 gaaataaagg cattttcata gaattaacaa tagtccttta tatagagact gttaattgaa
     4681 tattggagag aaaatgtcta actcaaatcg aacaaaatta cttttggata ttggtacgtt
     4741 catcgccttt ctcattacca tggacccgcg cttcagtgga ctggcaattc atgagtggct
     4801 atcacttgcc ctggctggca caattattgt ccatctctta ctgaactggg attggatcgt
     4861 aaatgttacg agacgcttat ttggcaaagc gaatgcaaat gcacgtgtca actatgtgtt
     4921 gaattggttg ctgttcatcg atgggattct catcatgctg tctggcatca tgatctctga
     4981 agttgccatc ccgtctttgg ggctggcgtt gcctatgaac tttgcctggc gcagattgca
     5041 cgacatgtct gccaatcttg cgttgctcat tatgggcttg caccttgcca tgcattggaa
     5101 ttggattgtg accaccttca aacgagtctt cttgggcggt ttcaagaaga aacaaccagt
     5161 tattgccgcc attgaggaag cttaatgatg aatacattat ttcgggtatt aataatttta
     5221 gtgatcgcca cgatcattgg cggattaatg tatgtgggtg tgagcacttc aaattcatca
     5281 accaatggtt ttcctgaatt tgaaggcgga cgtgaacgcc cgccattcgc tgaaggttcc
     5341 gagttccgcc cagatggcga caatgatgga gaacgtggtg agcgcggcgg ctttggattt
     5401 cctggtggca tcatcaaagc tgtagtatta atgtctgttg ctggcggtat ctattcagga
     5461 attgtttggg cgggcaagaa aacaaagcaa acgctggcgt agaaataata taaaagagga
     5521 cgtgctaagc acgtcctctt ttcgttagat cttggggatt tcaataatta ctttcaaccc
     5581 tttgcctttt tcgctttcag cccaaatacg ccccttatgc atttctacaa tagacttggc
     5641 aatagcgaga cccaagccag agccaccatc gtcacgcgtg cgggattcat ctacgcggta
     5701 gaaacggtca aataggtgag tggcttcttc aggatgaacc ccatcgccgc tatctttaac
     5761 ggagagctgg atgctgcttc ctgtaagctg agttttcaat tccacgcgtc cgccttcggg
     5821 ggtgtggcgg agtgcattgt ccagaatatt ttttaggact tgagcaaaac ggttcgggtc
     5881 gagatttgcc gtcagggttg ttgaggcagt ttcaaggtcc agtgagattc gtttttgatt
     5941 gaatagagtc aagtaatgag cgtggatgtc gctcataaag ttattgatgt ctataggttg
     6001 aaaatccaca gaaagttcac ccgcatcggc gagc
//
LOCUS       DAOREF010000043         5985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7075_length_5985_cov_4.428162, whole genome shotgun sequence.
ACCESSION   DAOREF010000043 DAOREF010000000
VERSION     DAOREF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7075_length_5985_cov_4.428162"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKI33_01510"
     CDS             <1..183
                     /locus_tag="PKI33_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM35703.1"
                     /translation="TTTRKVGGTGLGLPISRRLVEMHGGRLWAESSGIDGEGSTFFVE
                     LPLETRVTEIIEKREK"
     gene            184..1839
                     /locus_tag="PKI33_01515"
     CDS             184..1839
                     /locus_tag="PKI33_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNM35704.1"
                     /translation="MAMPLYLECNLCNHQQAYIPFVPAICEKCESQWVEARYDYESFK
                     REILRGIPNRPNNLWRFQDVLPLKNPSALDLYPAGGTPLWLSQRFAPDLGHGSVYIKD
                     ERYSPTSSFKDRQAAVAVAAMNENGIREAVIASTGNAAVAYAAACARAGIKLWVFMTS
                     RVPQEKLREAALFGAEVIRVSGNYDQTKQIASQFAQRKNLLLDRGASSVPARESMKTI
                     AYEIVEQLGWRAPDWYIQAVSGGLGPLGVYQGFKEMFNMGLIKKVPKLAIIQSAGCAP
                     MVNAFKEGKDVATPVIPDTRITILSTGDPGKAYTYLWNLTQQYGGVMESVTDLEAYEA
                     MKTLAKSEGMAVEPATSVAFAGFQKLSGQGIISKDELVAINCTGHTFPVEKHVLGDQW
                     AVDVHLSKDQSPAPHEGLQAALENLDEKTTTILLVDDNSDDALLIRRLLEGRKNYRMY
                     HAKDGWEGLSVARQKLPDLIVSDLTMPGIDGFGLVEELKLDPRTKDIPVVVVSAKDIT
                     KEERLRLNGQIEALYQKGSLPTRKFVDKVIGVIEDKFEEKGKE"
     gene            1849..>2180
                     /locus_tag="PKI33_01520"
     CDS             1849..>2180
                     /locus_tag="PKI33_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM35705.1"
                     /translation="MGNIILCIEDNPDNMMLVKRALESRGYVLLEAFTGNKGVEVAEQ
                     NNVDLILLDINLPDIDGYEVARRLRSSSKHNLAYTPIIAVTANALKGDAEKALAAGCD
                     VYMSKPIN"
     assembly_gap    2181..2280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2281..>3522)
                     /locus_tag="PKI33_01525"
     CDS             complement(<2281..>3522)
                     /locus_tag="PKI33_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNM35706.1"
                     /translation="TLILGFTPQIFTIISELVAANESRKSGAVIVVLADDDKVDMEDA
                     IKERIPDTKNTRIICRSGSPMDMTDLEIASPHTARSIIILADGDDPDTRVIKSVLAIT
                     NNPNRRNEPYHIVTQIRDPQNMDVIKLLSEKDTVQPILTTDLIARVVAQTSRQSGLSV
                     VYTELMNFGGDEIYFKQEPGLSGKTYGDALLSFETSTVMGIRRADGTIMMNPAMDTRI
                     EPGDSIFAIAEDDDKIQLTSSRLTLEENLIQRSGNRSKPKPERGLILGWNRSGSIIIR
                     ELDNYVAKGSELLVVSDVDGLESQIQREVGKLKNQKLKVMEGDIRDRALLDELGVTEY
                     DHVIVLAYSHLDVQDADAITLVTLLHLRDIAEKDETPFSIISEMLDLRNRELAEAAKV
                     DDFIVSEHLISLMMAQLSENAE"
     assembly_gap    3523..3622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4045..4686
                     /locus_tag="PKI33_01530"
     CDS             4045..4686
                     /locus_tag="PKI33_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNM35707.1"
                     /translation="MFQFEGFDRRKQEDKVREEGRLPPGQSLTQKFPVLHYGPVPTFN
                     PAAWDFRVWGEVEEEKRWSWDEFNQLPRTKLHMDIHCVTRWSKFDTDWEGVSVKTLVE
                     QGFFKIKPTATHVMQHAEYGFTVNLPLEVVLQENFLLATHFQGEPISPDHGYPLRGVV
                     GFIPGQEELETPYFWKGAKWLRSLEFMPRDKRGFWEQAGYHNRADVWREERFG"
     gene            complement(4730..>5985)
                     /locus_tag="PKI33_01535"
     CDS             complement(4730..>5985)
                     /locus_tag="PKI33_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNM35708.1"
                     /translation="QGTDSEVFVVQTTGTDRRSIPYQVLGVQSMMKYKLVIPVLVLAV
                     LACQTPVFTAQQEPADTTNQSTTSEVIPTLAAPANLDASNPAAAQDVLVALFENVSPG
                     TVAIFTETGQGSGFVYDTQGHIVTNFHVVSQDATTAVDIVEVRFPSGFMAYGSIVGID
                     LDSDLAVIKVDAPAEELKPVPLGDSSSLKVGQTVVAIGNPFGLQSTMTVGIISALGRT
                     LDSERSSPDGNFFSAGDIIQTDAAINPGNSGGPLFNLNGEVIGINRAIRTFSQNDAGE
                     PVNSGIGFAISINIVKRVVPVLIETGNYDYPYLGVSSSSGLSLDAIKALGLPQFTGAY
                     VSSVVPGGPADRAGLKAGDQPTNFPGLMAGGDLIIAIDGREVRTFDDLLAYLITNKGP
                     GDTVVLTVLRGTEKVDISIVLDKRP"
BASE COUNT         1547 a         1376 c         1451 g         1411 t
ORIGIN      
        1 accaccaccc gcaaggtcgg tggaacaggg ttgggtctgc cgatcagccg ccgccttgtc
       61 gaaatgcatg gtggccgtct ctgggcagaa agttccggca ttgatggtga aggctctaca
      121 ttctttgtag aattgcccct tgaaactcgc gttacagaaa tcattgagaa aagagaaaaa
      181 taaatggcaa tgccgcttta cctcgaatgc aatctttgca accatcaaca agcctacata
      241 ccattcgtgc ctgccatttg tgaaaaatgc gaatctcaat gggtcgaagc tcgttacgac
      301 tacgagtcgt tcaaacgtga gatcttgcgc ggtattccca accgcccaaa taacttgtgg
      361 cgctttcagg atgtcttgcc attaaagaat ccatctgcgc ttgacctata ccccgcaggt
      421 ggaactccac tctggctctc acaacgcttc gctccggacc tcgggcatgg ttctgtttac
      481 atcaaagatg aacgctacag cccgacctct tctttcaaag atcgtcaagc cgcggtggca
      541 gtggccgcca tgaatgaaaa tggcattcga gaagcggtca ttgcctccac agggaatgct
      601 gctgtagcct atgcagccgc ttgtgcacgt gcaggcatca aactatgggt tttcatgacc
      661 agccgtgtgc cacaggaaaa attgcgagaa gctgccctct ttggtgcaga agtgatccgc
      721 gttagcggca actacgacca gaccaagcaa atcgccagtc agttcgcaca acgcaaaaat
      781 ctgttgctcg accgcggcgc aagttctgtg cctgcccgcg aatcgatgaa gaccattgcc
      841 tatgagatcg tagaacagct cggctggcgt gcccccgatt ggtatattca agccgttagc
      901 ggcgggttgg gaccgttagg cgtttaccaa ggtttcaaag aaatgttcaa catggggctg
      961 atcaaaaagg tgcctaaact tgccatcatc cagtctgcgg gttgcgcacc catggtcaac
     1021 gccttcaagg aagggaagga tgttgccacg ccagtcattc ctgatacacg catcaccatc
     1081 ctatccacag gcgaccccgg taaagcatac acctacttgt ggaacctcac tcagcaatac
     1141 ggcggcgtga tggagtccgt tacagacctt gaggcatacg aagccatgaa aaccctcgca
     1201 aaaagcgaag ggatggcagt ggaacccgcg acttctgtag catttgcggg ttttcaaaag
     1261 ttatctgggc agggtatcat cagcaaagat gagcttgttg ccatcaactg cacagggcat
     1321 accttccccg tagaaaaaca cgtcttggga gatcaatggg cagtggatgt gcatcttagc
     1381 aaagaccagt cccccgctcc acatgaaggg ttgcaagccg cgctcgaaaa cttggatgaa
     1441 aagaccacaa ctatcttatt agtggacgat aactcagatg atgcactgct tatccgccgt
     1501 ttgctggaag ggcggaagaa ctaccgcatg tatcacgcca aagatggctg ggaaggtctc
     1561 tctgttgcac gacaaaaatt gccagacctg atcgtaagcg acctgaccat gccaggtatt
     1621 gatgggtttg gtttggtaga agaattaaag ctagacccac gtacaaaaga tattcctgtt
     1681 gtggtcgtta gcgcaaaaga tataacaaaa gaagaacgcc ttcgcctcaa cggtcaaatt
     1741 gaggcactat atcaaaaagg atccctcccc actcgaaaat tcgtggataa agttatcggg
     1801 gtcattgaag ataaatttga agaaaaaggc aaagaataag gagaacttat gggaaacatc
     1861 atcctctgca tagaagataa tccggataat atgatgttgg ttaaacgcgc actcgaatca
     1921 cgcgggtatg ttcttcttga ggctttcaca ggaaacaaag gcgtggaagt agcagagcaa
     1981 aataatgtcg atctgatttt gttggatatt aacttaccag atattgatgg ctacgaagta
     2041 gcaaggcgct tacgctccag cagcaaacat aaccttgcat acactcctat cattgccgta
     2101 acagccaatg cgttgaaagg ggatgctgaa aaagcactcg ccgcaggatg cgacgtatac
     2161 atgtccaaac cgatcaatat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 ttcagcattc tcagaaagct gagccatcat caaactgatg aggtgttcgc tgacgatgaa
     2341 atcatccact ttggcggctt cggcaagttc gcggttgcgc aggtcgagca tttcgctgat
     2401 gatggagaag ggcgtttcat ccttctcggc aatatcgcgc aggtgcaaaa gagtaacgag
     2461 agtgatcgca tctgcatctt ggacatcgag atggctatac gctaacacga tgacatggtc
     2521 atattcggtc acgccgagtt catcaagcaa ggcacggtcg cgaatgtcac cttccataac
     2581 cttgagtttc tgattcttga gtttgcctac ttcgcgctga atttggcttt cgagtccatc
     2641 tacatcagac acgaccaaaa gttcagagcc tttggcaaca tagttatcca attcgcgaat
     2701 gatgatggaa ccagaacgat tccagccgag gataagtccg cgctcaggct tgggcttgga
     2761 gcggtttcca gaacgctgaa tcaggttttc ttccagagtg aggcgagatg acgtaagttg
     2821 aatcttgtca tcatcctcag caatcgcgaa gatggagtca ccgggttcaa tgcgggtgtc
     2881 cattgcgggg ttcatcatga tggtgccatc ggcgcggcga atacccatca ctgtggaagt
     2941 ttcaaacgac agaagagcat cgccgtaggt ttttccagag agaccgggtt cctgcttgaa
     3001 ataaatttcg tcgccgccaa agttcatcaa ctcggtgtag accacagaca aaccgctttg
     3061 gcgggaagtt tgcgctacga cgcgggcaat gaggtcggtg gtgagaattg gctggacggt
     3121 atctttttca gagagcagtt tgatcacatc catattttgc gggtcacgaa tttgggtgac
     3181 gatgtgatac ggctcattgc gacgattggg gttgttggtg atcgccaaga cagatttgat
     3241 aacgcgggta tctgggtcat cgccatcggc aaggataata atagagcgcg cggtatgcgg
     3301 gctggcgatc tcaagatcgg tcatatccat tgggctgcca gaacggcaaa tgatgcgagt
     3361 gttcttggta tcagggatgc gttctttgat ggcatcttcc atatccactt tatcatcatc
     3421 tgccaagacg acgataacgg caccactctt gcggctttca tttgcggcaa ccaattcgga
     3481 gatgatggta aaaatttgcg gagtgaaacc aagaatgagg gtnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnccattggg ggctgtgttt gtcagtttga caagagcggc
     3661 ggctgcaaaa atgataatgg cagaaagaac aaataagcca aataataacg cgggtgtccc
     3721 tttggacatg tagttgtcga accaatattg aaatctctgt ttgaatgtgg gctgcttcat
     3781 aaaactatct ccttgcgcct ttgaaaattt ggcttattat aagagcaatt gcatctatga
     3841 ccagagggta ttttcattac atttgattta catattttgc agatggtctt aaacgacaga
     3901 ggtcactgag aatttaagaa aagcatttcg ggcaattcaa agctctaact tcttatacaa
     3961 ttattagttt tgcagggtaa catctggcgt ttctttcatg tctattccat gccagtaggc
     4021 agactttagt aaagtgaggt aaccatgttc caattcgaag gttttgaccg ccgcaaacag
     4081 gaagataaag tccgtgaaga ggggcgtctt ccccccgggc aatcattgac ccagaaattt
     4141 cccgttttgc actatggacc cgtccctaca ttcaaccccg ccgcatggga ctttcgtgta
     4201 tggggtgaag tggaagaaga aaagcgctgg agttgggatg aattcaatca actgccacgc
     4261 accaaacttc atatggacat ccattgtgtc acccgttgga gcaagttcga taccgattgg
     4321 gaaggggttt cggtaaaaac tctggtggaa cagggtttct tcaagatcaa accgaccgcc
     4381 acacacgtga tgcaacatgc tgaatatggc tttacggtca acttgccctt ggaagtagta
     4441 ttgcaagaaa acttcctgct cgccacccat tttcaaggcg agccgatctc acccgaccat
     4501 ggttatccat taagaggcgt ggtggggttc atccccgggc aagaagaatt ggaaacgcct
     4561 tatttttgga agggtgccaa gtggctgcgt tctttagaat ttatgccgcg cgataaacgc
     4621 ggattttggg agcaagccgg gtatcacaac cgggctgatg tttggcgcga agagcgtttt
     4681 ggttaaataa aaaaactccg aagtcttaag gacttcggag tttttttatt tatggtcgtt
     4741 tgtccaacac aatcgaaata tctacttttt ccgttccacg cagaacggtc aacaccacag
     4801 tatcgccggg tcctttgttg gtgatgagat acgctaacag gtcatcgaaa gtgcgcactt
     4861 ctctaccgtc aatggcaatg atcaagtcac cacctgccat taagccgggg aagtttgtgg
     4921 gctgatcacc agctttcaac ccagcacggt ctgcgggacc acctgggaca acggagctta
     4981 catacgcgcc agtaaattgc ggcaagccca aagctttgat cgcatcgagg ctaagtccgc
     5041 tgctggagga gacacccaag tacggataat cataatttcc agtttcgatc aaaaccggca
     5101 ccacacgttt gacgatgtta atcgagatgg caaacccaat gcccgagttg accggctctc
     5161 ccgcatcatt ctgactaaat gtacggatcg cgcggttgat accaatcact tccccattca
     5221 aattgaaaag cggaccaccc gaattgccgg ggttgatcgc ggcatcggtt tgaataatat
     5281 cgcctgcgct gaaaaagttc ccatctgggc tggagcgttc agagtctaat gtgcgaccca
     5341 aggctgagat aatgcccacc gtcatggtgc tctgcaagcc aaacggatta ccaatggcaa
     5401 ctacggtctg acccactttg agtgaggatg aatcgccgag cggcacaggc ttgagttctt
     5461 ctgccggcgc atccactttg atgacggcga ggtctgaatc caagtcaatg cctacaatgc
     5521 tgccatacgc cataaagccg gaaggaaaac gaacttcaac aatatccact gctgtggttg
     5581 catcttgcga aaccacatgg aagtttgtaa caatatgacc ttgagtatca tatacaaaac
     5641 cagagccttg cccagtttcg gtaaagatcg ccactgtgcc agggctcacg ttttcaaaca
     5701 aagccaccaa cacatcttgt gcagcggctg ggttcgaagc gtcaagattc gccggagccg
     5761 ctaacgtagg aatcacttct gaagtcgtgg attggtttgt ggtgtctgcc ggttcttgtt
     5821 gtgcagtaaa aaccggtgtt tggcatgcaa gcacagccaa caccaaaacc gggatcacga
     5881 gcttgtattt catcatggat tgaactccta aaacttgata cgggatgctt cttcgatcag
     5941 ttcccgttgt ttggacgaca aatacttcgg aatctgtacc ttgag
//
LOCUS       DAOREF010000044         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7110_length_5963_cov_4.103758, whole genome shotgun sequence.
ACCESSION   DAOREF010000044 DAOREF010000000
VERSION     DAOREF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7110_length_5963_cov_4.103758"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1865
                     /locus_tag="PKI33_01540"
     CDS             <1..1865
                     /locus_tag="PKI33_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35709.1"
                     /translation="VSHIRYQGFQGNIRATTRPISFDQQALGQIMALPELASWYSNGG
                     LLVSDNLGSTAVHEFYAAGGSQFQARIAARDSFIAGNDLLYLGNIKSDDTPDSYTSVI
                     RTIDFFAQKYREDPAFAQRVDASVARILTAKLKLYENFDIGNVIVPAVNLAQSDAGSN
                     ITFQVARSSATLISPSQQDLASLLPLPPQPSDRIVFITDSVSVAQCTECAAQPLLATD
                     ALQNVVLRLYGPQSGNQVEEFRLKSYSFDNVSAWLDSPDENQFVADDLDNADWVVISI
                     TDSLRSQPALITRLFRERPTLLLNKQLILFSFGAPYYFDTTTLSRFTAYYALYSKQPQ
                     FVDVAVRLLFQELTPAGASPVSIPAVGYDLIDVTSPSPIQIIPIFLDLPPDPATPNTG
                     ILPESTLTPLFSIGDTISLRTGVIKDHNGNPVPDGTVVRFSMLLTGEGGGILQQVESI
                     TTDGVARAAFGLDKPGLLEIRVASEPAVISEVLQLDVSQTGEVAVTVVVPELTQSSVP
                     TATPEAVEEADAFVTQEGYPRVSAWSLAMLFVLLGSGALYVIASRFTKRMIATRWSLG
                     MLLGGLFVYNLMAFGFFDLPSWLLEAGISGVLYATLTGQLLGFAAGWAWSRLEK"
     gene            1946..2971
                     /locus_tag="PKI33_01545"
     CDS             1946..2971
                     /locus_tag="PKI33_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNM35710.1"
                     /translation="MESNGKAGKNKVIKAKQPPILFDKTQAIIADLTKKLGGTLVTYF
                     NNPRGAVCHDDVLALFELLEQIGKQEKIYLFLKSSGGNGQASLRIVNLLRQYCEELIA
                     VIPLECASAATMITLGANEIHMGPMAYLTSVDTSLTHALSPIDRDNDRVSVSLDELNR
                     VVKLWQAQRSDSNENPYQQLFQHVHPLVIGAVDRAESLSIMLCKELLAYHIKDEQVGE
                     KIASTLNSKYPSHGYPILYEEAHRIGLKVSRLSPEINSQLLELNELYSEMGQRATTDF
                     DDTHAHGNEILNIWESTDVLVYYQQDKDWFYRTEERRWISLNDNSSWRRMHKKGNKVE
                     KSILHIS"
     gene            3097..3696
                     /locus_tag="PKI33_01550"
     CDS             3097..3696
                     /locus_tag="PKI33_01550"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HNM35711.1"
                     /translation="MATNIHLKFNDQAPDLTLLDGAGNPVQLSSLWKKQVLVLAFTRH
                     FGCPQCKEMVDQLIDSHQTLTEAGLLLAIVSHASPESAKAFCDQRAPNALCLADPDRT
                     VYKAYGLYRGTLWQTILSPNIWKSNRRLARQKGIKPGVPSPGQDVYQMSGTFVIGMDG
                     KIRLPYYYEDIADHPPVDLLLHGIMGVDWKKPFDSNPLV"
     gene            3710..4870
                     /locus_tag="PKI33_01555"
     CDS             3710..4870
                     /locus_tag="PKI33_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35712.1"
                     /translation="MKTFFLATYLALKEVFRNRGRFLLVALVIALITMLVLFIAALGE
                     GLGNGNRQYIANLDADLIVFLEKSDYIISASRLEVNTARSIRRVEGVADAGAIYTSST
                     EIVSLEEPLKVSMLGVEPGRPGMPTILEGREFRTGEAREAVIDRNVALRSDIKIGDTI
                     RIRSTQGTDDEFYELKVVGLVDGQSYTFQPTIFVPPATWEKIRPQSESDMVSDTPYPN
                     VVAVKLQDPTQIDAMSARLMERIPNIEVADIQTTISNIPGYSAQQGTVQTQAVFTLLI
                     GTLVIGGFFQIQILQKVPQIGVLKAIGSANGMVGLSAVIQIIVVTAMGVGIGSGLTYF
                     FSLGFPPTIPIVFNGVNSLIAVILLLLIGPIGGMVSIIYAVRIEPLKALRLG"
     gene            4873..5601
                     /locus_tag="PKI33_01560"
     CDS             4873..5601
                     /locus_tag="PKI33_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM35713.1"
                     /translation="MSDIALEVRDLVKTFSLDGSTINAVDHVSFQVKFGEFVALVGPS
                     GSGKTTMLSILAALLTPTSGQVLIDGQDLSKMSEKERVKLRREKIGFTFQANNLIPYL
                     SARENVEFMLRLNGKLDHASRIRSDELLARLGLAERLHNLPAQMSGGQQQRVAIARAL
                     IHNPALVLADEPTASLDTERAFQVVETFANLIHENGRAGIIVTHDLRMCQYVDRVFQM
                     QDGKLIRTYETKNEIMELATATVH"
     gene            complement(5649..>5963)
                     /locus_tag="PKI33_01565"
     CDS             complement(5649..>5963)
                     /locus_tag="PKI33_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNM35714.1"
                     /translation="LGQMPVVEFDATWCPPCQAIDQSIGEENELMMNAYDGTYIIKLD
                     VDVWGWDNGKVEMFQFEAIPVYFKLDTSGKPNGEVIDGGAWGEDIPENIAPVMDAFFH
                     NK"
BASE COUNT         1465 a         1388 c         1431 g         1679 t
ORIGIN      
        1 tggtttctca tattcgttat caagggtttc aagggaatat ccgcgcgacc acacgcccga
       61 tcagttttga ccaacaagcg ttggggcaga ttatggcgtt gcctgaattg gcaagctggt
      121 acagcaatgg tggcttgttg gtgagcgata atctcggaag cacggcggtt catgagttct
      181 atgccgcagg tggaagtcaa tttcaagccc gtattgcagc gcgtgattct tttattgctg
      241 gtaatgactt gctttatctt ggcaacatta agagcgatga tacgccagat agttatacat
      301 ccgtcatacg gaccattgat ttctttgcgc agaaataccg tgaagacccc gcctttgctc
      361 agcgtgtaga tgcttcagtg gcccgtattc tgacggcaaa acttaaactg tatgaaaatt
      421 ttgatattgg aaatgtcatc gtccctgctg tgaacttggc tcaatcggat gcaggaagca
      481 atattacatt tcaagtggcg cgaagttctg caacattgat cagccctagc caacaagacc
      541 ttgcttctct tttgccttta ccgcctcagc ccagcgaccg aattgttttc ataacagact
      601 ccgtcagcgt ggcacaatgc acagaatgtg cggcacagcc acttcttgcg accgatgcgt
      661 tgcagaatgt cgttttgcgt ttgtacggtc cacagagcgg caatcaggta gaagagttcc
      721 ggcttaaatc ctattcattt gataatgtaa gtgcctggtt ggattctccg gatgaaaacc
      781 aatttgtggc agacgatttg gacaatgccg attgggtggt aatttccatc actgactcat
      841 tgcgttcgca accagcactg ataactcggt tgttccgcga acgcccaact ttgctactga
      901 acaaacagtt gattttgttc tccttcggcg cgccgtacta ttttgatacc actacgctct
      961 cgcgttttac ggcatattat gccttgtata gcaagcaacc tcaatttgtt gacgtggcag
     1021 ttcggttgtt attccaagaa ctcacgccag cgggagcgtc tcctgtttca attcctgcgg
     1081 ttggatatga tttgatcgat gtcacatcgc ccagcccgat tcagatcatt ccgatctttc
     1141 tggacctgcc gcctgacccg gcaaccccaa ataccggcat cctccccgaa tcgacgttaa
     1201 ctccgctgtt tagtattggc gatacgattt ctcttcgtac tggggtgatc aaagaccata
     1261 atggaaaccc agtccctgat gggactgtgg ttcggttctc gatgttattg actggcgagg
     1321 gcggtggcat tttacagcaa gttgaatcga taacgaccga tggtgtggcg cgtgcggcat
     1381 ttggtttgga taaacccggt ctattagaga ttcgagttgc cagcgaacct gctgtgatct
     1441 ctgaagtatt acagttggat gtctcacaga ctggtgaagt cgcggtgacg gtggtcgtgc
     1501 cagagttgac acaatcttct gtgccaactg ccacgcctga agcggtagaa gaggcggatg
     1561 cctttgtcac gcaggaaggc tatccgcggg tttcggcttg gtctcttgcc atgctctttg
     1621 ttttgctggg gtctggcgct ttgtacgtga tcgcttctcg ttttaccaaa cgaatgattg
     1681 ctacacgttg gtcgttagga atgttgttag gtggtttgtt cgtatataac ctgatggcat
     1741 ttggcttttt tgaccttcca agttggttgt tagaggcggg aatatctggc gtgctgtatg
     1801 ccacgcttac aggtcagtta ttaggttttg ctgctggctg ggcttggtca cgcctggaga
     1861 aatagtacta tcaacttatt ttgtttctag cgttcagtgt taccatttat tggattgata
     1921 tcattaaggt taggagacga attatatgga atctaacggc aaagctggca aaaataaagt
     1981 gatcaaagca aagcaacctc cgattctgtt tgacaagact caggcaatca ttgcagactt
     2041 gaccaaaaaa ctcggcggca cgcttgtcac gtatttcaac aatccgcgcg gtgcggtttg
     2101 ccatgacgat gtgttggctt tatttgaact gctcgaacag attggcaaac aggaaaaaat
     2161 ctatttattt ctaaaatcca gcggcgggaa cggacaagct tctttgcgta ttgtgaatct
     2221 tttgcgccaa tactgtgaag aactaattgc tgtgattcct ctggaatgtg cttcagcggc
     2281 gaccatgatt acattgggcg cgaacgaaat ccatatggga cccatggcat atctcacttc
     2341 agtggatact tctttgacgc acgcattatc tcccattgac cgcgacaacg accgcgtgag
     2401 cgtcagcttg gatgaattga atcgtgtcgt gaaactgtgg caagctcaaa ggtccgactc
     2461 gaacgagaac ccttatcaac aactctttca gcatgtgcat ccgcttgtga ttggcgccgt
     2521 ggaccgtgct gagtctcttt ccattatgtt gtgcaaagaa ttacttgcct atcacattaa
     2581 agatgagcaa gtgggtgaga agatcgcatc gacacttaac tctaaatatc cttcgcatgg
     2641 gtatcccatt ttgtatgaag aagcacaccg tattggcttg aaggtaagcc gcctttcacc
     2701 agaaatcaat tcgcaattgc ttgagctcaa cgagctttac agcgaaatgg gtcaacgcgc
     2761 tacaacagat tttgacgaca ctcatgcaca tgggaatgag attctcaata tttgggaatc
     2821 gaccgatgtc ttggtgtatt accagcaaga caaagattgg ttttatcgca ctgaagagcg
     2881 ccgctggatt tcgctcaacg ataatagcag ttggcggcga atgcacaaaa agggtaacaa
     2941 ggtcgagaaa tcgatcctgc atatctcgta agaatttgag gcacagtagg gaactgtgcc
     3001 tctttttttg gttgggggag ggtaaaaaca actcgttgca aaacctgtac aaaacctgtg
     3061 cattgtgcta aaatctcgtc attcaaaaag gtaactatgg caacaaatat tcacctcaag
     3121 tttaatgacc aagctcccga cctaaccctc ttggacggcg caggcaaccc tgtgcagctt
     3181 tcatctctct ggaagaagca agtccttgtg ctggcgttta cccgtcactt tggatgcccg
     3241 caatgcaaag agatggtgga tcaattgatc gactcgcatc agactctgac cgaggctggg
     3301 ttgctgctcg caatcgtttc tcacgcctca cccgaatcag ccaaagcgtt ttgtgaccag
     3361 cgtgctccga atgcgttgtg cctcgctgac ccagaccgta ccgtttacaa agcctatggt
     3421 ctttaccgtg gcactctctg gcaaaccatc ctttccccca atatttggaa gtccaataga
     3481 aggttggcgc gacagaaagg catcaaacct ggtgtaccat cccccggaca ggatgtttat
     3541 caaatgtctg gcacctttgt tattggtatg gatgggaaaa ttcgtttgcc ttattactac
     3601 gaagatattg ccgatcatcc acctgtagat ttgcttttac atggcatcat gggtgtggat
     3661 tggaagaaac cgttcgactc aaaccctttg gtctaggaag tacgaaataa tgaaaacttt
     3721 ttttcttgca acttaccttg cgctcaagga agtgttccgc aatcgcggac gttttttgct
     3781 tgtggcgctc gtcatcgcgt tgatcacgat gttagtcctc ttcattgctg cattagggga
     3841 ggggttgggc aatggtaacc gccaatatat tgccaactta gatgcagacc tgatcgtctt
     3901 tttggagaaa tcagattaca ttatctctgc cagccggttg gaagtaaaca ctgctcgttc
     3961 cattcgacgt gtggaaggtg tggcagatgc tggcgcgatc tatacatcca gcacagaaat
     4021 cgtgtcgtta gaagaaccct tgaaggtatc aatgctgggt gtagaaccag gtcgccccgg
     4081 catgcctact attttggaag ggcgtgaatt ccgtaccggt gaagcgcgtg aagccgtgat
     4141 tgaccgcaac gtagctttgc ggtcagatat taagataggc gatacgattc gaatccgctc
     4201 cacccaaggc accgacgatg aattttatga acttaaggtc gttggcttag ttgatggtca
     4261 atcatatacc tttcagccca ctatttttgt cccgccagcc acatgggaaa agattcgccc
     4321 tcaatctgaa tcagacatgg tcagtgatac accgtatccc aacgtggtag ctgtgaagtt
     4381 gcaagaccct actcagattg atgcaatgtc tgcccgtttg atggaacgta tacccaatat
     4441 tgaagtggca gatattcaaa cgaccatcag taatattcct ggatattctg cccagcaagg
     4501 gacagttcaa acgcaggcgg tctttacttt attgatcggc actcttgtta tcggcggctt
     4561 cttccagatt cagattcttc aaaaggttcc gcagatcggt gtgttgaagg caattgggtc
     4621 tgccaatgga atggtaggtc tttctgcagt gatccagatc atcgttgtga ccgccatggg
     4681 ggtgggcatt ggtagcggat taacctattt cttctcgctt gggttccctc caactattcc
     4741 gattgtgttc aatggtgtta attcactcat cgctgtgatc ttgcttctgt tgatcggacc
     4801 gatcggtgga atggtctcta tcatttatgc tgttcgtatt gagccgctca aggcacttag
     4861 gttagggtaa atatgtcaga tattgcattg gaagttcgcg atttagtcaa aaccttctca
     4921 ctcgatggct cgaccatcaa cgcagtagat catgtatcgt ttcaggtaaa gtttggtgag
     4981 tttgtggcgt tggtcggtcc tagtggttcc ggtaaaacga ctatgctctc catccttgct
     5041 gccttactta cacccactag cggacaagtc ctcattgatg ggcaagacct ttcaaagatg
     5101 agcgagaaag aacgtgtcaa actgcgccgc gagaagatcg gttttacctt tcaggcgaat
     5161 aatttaattc catatctttc tgctcgtgag aatgtggaat ttatgctgcg ccttaacggc
     5221 aagctggatc atgccagccg catccgctct gatgaattac ttgcccggct tgggttggct
     5281 gaacgcttac acaacttgcc tgcacaaatg tctggtggtc agcagcaacg tgttgctatc
     5341 gcccgggcgt tgattcataa ccctgcactt gtacttgccg atgaacccac cgcttcactc
     5401 gatactgagc gtgctttcca agtagttgaa acctttgcaa atctcatcca tgaaaatgga
     5461 cgtgccggta tcatcgtgac gcacgacttg cgtatgtgcc aatatgtaga ccgtgtcttc
     5521 cagatgcaag acggtaaact gatccgcacg tatgagacta aaaacgaaat tatggaactg
     5581 gctacagcga cagttcacta aattggttat aaataaaaag ggctcacttc aaaagtgagc
     5641 cctttttatt atttgttgtg aaaaaaggca tccatgacag gtgctatgtt ttcgggaata
     5701 tcctcgcccc atgcaccgcc atcaatcact tcaccgtttg gttttccaga agtatccagc
     5761 ttgaaatata cagggatagc ttcaaattgg aacatttcta ctttgccatt atcccaaccc
     5821 cagacatcaa catccaactt gatgatatag gtcccatcgt aggcgttcat cattaattcg
     5881 ttctcttcac caatagactg gtcaattgcc tgacaaggtg ggcaccaggt cgcatcaaac
     5941 tcaacgacag gcatctgacc cag
//
LOCUS       DAOREF010000045         5948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7146_length_5948_cov_3.573732, whole genome shotgun sequence.
ACCESSION   DAOREF010000045 DAOREF010000000
VERSION     DAOREF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7146_length_5948_cov_3.573732"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PKI33_01570"
     CDS             complement(<1..1062)
                     /locus_tag="PKI33_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM35715.1"
                     /translation="MKHLKSVLFMMVISALLLAACGGGSAPADNTGGEATQAPGAGEG
                     NKTATFIFTQEFDNLNPLYTNQWFTAITYQLWSCYAWTYDDQNAPVPNLVTELPTTEN
                     GGISEDGRTLTFKLRDDIVWSDGEPITADDFVFTYEMTMDPANTVASQSPYDQMESVV
                     AADPQTVVVTFTEPFAPWAATLWKGIIPKHVLQPVFEADGNLNAAEYNRAPTVGCGPF
                     NFAEWEAGSFARFVANDNYWLGRPKLDEIFIRFVPDDASQIAALQAGEGDLGTFIAYS
                     DIPALEEAGVQMFTVFSGYNEGLYLNLGEKGHPALKELGVRQAIAYALDRESLVNDLL
                     LGRTGVAATYWDNTPFVDPS"
     gene            complement(1112..1918)
                     /locus_tag="PKI33_01575"
     CDS             complement(1112..1918)
                     /locus_tag="PKI33_01575"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNM35716.1"
                     /translation="MTLLMPIGGNENDKKPVVSKEFIRLAGGEKADIVVLPQPSVLED
                     TPEYYVQHFLSLGARSVKSLDFRSRAEAHLDEYVEVIQNATGIFISGGTQMRLPAIIG
                     GTNVETAIFEAYRRGVIVSGTSAGASVMSKVMIAYGKGGSTPREGIVRFTPGLGLTDK
                     IILDQHFRQRDRFGRLLYAVSAHPGLLGVGIDEDTAAIIEDEAKITVVGTGAVTVVDG
                     SELESTNVAEAEKGKPVAVSNLRIHVLTAGCSYHIPTRKAHIPNLQLVRE"
     gene            complement(1915..2589)
                     /locus_tag="PKI33_01580"
     CDS             complement(1915..2589)
                     /locus_tag="PKI33_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNM35717.1"
                     /translation="MNSDSHRQLRNVVADKMRAAILDGQYKPGEWLRQERLAQELGVS
                     QMPVREALNELVNEGLIEHVPYRGARVIQFSIEDVIDLYEHRAFLEGRAAYFAAQIIT
                     DRDLTALKKIQVGMEENDAPEAVLKYRDLNRNFHKTIFTISKHKYLIRTLNQMWAAFP
                     TMLIGNFAATASHPLPARDDTDRQEHQAIMDALFAHDGTSAEQAMRNHILATREQLVS
                     FLKANQ"
     gene            complement(2599..>3146)
                     /locus_tag="PKI33_01585"
     CDS             complement(2599..>3146)
                     /locus_tag="PKI33_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35718.1"
                     /translation="ARNVPIGGTSAGLAILGEISFSAANGTVDSSTALGNPFGRRIAL
                     ERDFLTVPYLSSLITDSHFVTRDRMGRLVTFLARIVNDGWAPQAKGLGIDEATAVVVE
                     ADGSASILGNGAAYFLQTPGTPEVCLPKTDLTYRNVSVYRISGSATFNFATWTGSGGT
                     AYTITAENGTVTSSQPGGSIY"
     assembly_gap    3147..3246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3247..3761)
                     /locus_tag="PKI33_01590"
     CDS             complement(<3247..3761)
                     /locus_tag="PKI33_01590"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNM35719.1"
                     /translation="MKLVFPFFRFTTLFIILVSLISPSPVFAAKKHYEYYVVGNSNNA
                     TSPTQAGTVLMGGGTDVDAAFQWMINKSGGGDFVVIRASGTDAYNPYIYGLGTVDSVE
                     TLIITSRTGASDPFVLDKINNAEALFIAGGDQADYVNYWKGTPVEDAIHNLIARNVPI
                     GGTSAGLAILG"
     gene            complement(4067..4798)
                     /locus_tag="PKI33_01595"
     CDS             complement(4067..4798)
                     /locus_tag="PKI33_01595"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM35720.1"
                     /translation="MRKPLFLAVMILIMLACAVPGAPAVAPLPTFDSNSLQTFIVQTA
                     EAAQTQTALVMPTFTKTPTTTFTPSITPSSTPTFIFKLSTPTPIPTYTIPPTIGTLKS
                     PVPESASGDDEENTDGDKPKNKDEDPRKMSGKDWTCVWYGLNPPRNTIFKPKTHFTVQ
                     WMLFNSGNQSWPRTGVDFVYTGGFRHEETKIQDFAQSVAPGKEIWVRASFIAPKAAGD
                     YSTYFTLTVGKRRFCSVAYYFTVQE"
     gene            complement(4811..>5948)
                     /locus_tag="PKI33_01600"
     CDS             complement(4811..>5948)
                     /locus_tag="PKI33_01600"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNM35721.1"
                     /translation="VTALFYQNPVDETLDIFKRENFRDLVRLASGLKHYDVISTVGIV
                     RDVKEELGDMYGSLEHISNTTKPLVLLVSDEDRFPDVLDMFEHLNGKEVGDKPFVIPY
                     FNPVSPLVMNAGTVDKMKVAIERGLPIIFSNYSMAGASTPLTPAGTLTLLMAELLAGL
                     VISQTIKKGAPILLGMLPVYFDMRTMLNFYDPQSILISVACSEMMKSYGIPHCSTSGS
                     GTGWGMDLIAADTYWMNTLALLLSNGHLAPFIGDSLGSKSISPTTFVHAHEIIDQALR
                     LHNGFQLDDANSAVDEIFKVGPGKSFLNQPSTLKNYKNGYYVSGVYPRYSMEKWMDAG
                     APPARQVLRQKTQDLMASAPVPNEYDEFIAKGEEFIRKRFHDIL"
BASE COUNT         1578 a         1386 c         1377 g         1507 t
ORIGIN      
        1 agacgggtca acgaacggag tgttatccca gtatgttgca gccacgccag tgcgacccaa
       61 cagtaaatca ttgacaaggg attcgcggtc aagtgcataa gcaattgcct ggcgaacacc
      121 caattccttc agcgcagggt gacctttctc gccaaggttc aggtacaaac cttcgttata
      181 gccagagaag acggtgaaca tttgcacgcc tgcttcttca agggcgggaa tgtctgagta
      241 ggcaatgaac gttccgagat caccttcacc agcctgaaga gcagcgatct gagaagcatc
      301 atcgggtacg aagcgaatga agatttcatc caacttgggg cgtccgagcc aatagttatc
      361 attggcaaca aaacgggcaa aagaaccagc ttcccattca gcaaaattga atggaccaca
      421 gccgacagtc ggtgcgcggt tgtattcagc ggcattcaag tttccatctg cttcaaacac
      481 aggctgaaga acatgctttg gaatgatgcc tttccaaagg gttgcagccc aaggcgcaaa
      541 cggttcggtg aaagtcacca ccacagtttg cgggtctgca gcgacaacgc tttccatctg
      601 gtcataggga ctctgagaag ccacggtatt tgcagggtcc atggtcattt cgtaggtgaa
      661 tacgaaatca tcagcagtga taggctcacc atcagaccat acgatatcat cgcgcaactt
      721 gaaggtcagg gtgcgaccat cttcagagat gccgccattt tcggtggtag gcagttcagt
      781 caccaaattc ggaacagggg cgttttgatc atcataggtc catgcatagc agctccagag
      841 ctgatatgta atggcagtga accactgatt ggtgtagagc gggttcaggt tatcgaactc
      901 ctgagtgaag atgaaggtgg cagtcttgtt gccctcacca gcaccaggtg cctgtgttgc
      961 ttcgccaccg gtattatccg ccggggctga gccaccgcca cacgctgcta gcagcaatgc
     1021 agaaatgacc atcatgaaca gaacagactt tagatgtttc atgcttttct tctcctttgt
     1081 gttatttgaa aacatgtgaa ccgttcacat gctactcacg aaccaattgc aggttgggga
     1141 tatgtgcttt gcgtgttgga atatgatatg aacaacctgc cgttaaaaca tggattctta
     1201 gattgctgac cgcaacgggt tttccttttt ctgcttctgc cacattggtg gattcaagtt
     1261 cgcttccatc tacaacggtc actgctcctg tacccacaac cgtgatcttc gcctcatcct
     1321 caatgatcgc agctgtatcc tcgtcaatgc cgacgccgag caaacccgga tgtgccgaaa
     1381 ctgcatagag caaacgtcca aaccgatcac gctgccgaaa atgttgatcc aaaataattt
     1441 tatcggttaa tcccaagcca ggggtaaacc gaactatccc ttcgcgcggc gtggaaccgc
     1501 cttttccata agcaatcatc accttagaca ttacagatgc cccagcactt gttcccgaaa
     1561 caatgacgcc acgccgatat gcctcaaaga ttgctgtttc aacgtttgtc ccaccgatga
     1621 tggcaggcaa acgcatttgg gtgccgccag aaataaaaat acccgttgca ttttgaatga
     1681 cctcaacata ttcatccaag tgcgcctcag ctcgtgagcg aaaatctaaa ctcttcactg
     1741 aacgagcacc aagacttaag aaatgctgaa cataatactc tggagtatcc tctaaaaccg
     1801 acggctgtgg aagcaccaca atgtcggctt tttcccctcc tgcaaggcgg ataaactctt
     1861 tcgaaacgac tggtttcttg tcgttttcgt ttccaccaat cggcattaac agcgtcattg
     1921 attcgccttt aagaatgaaa ccaactgctc acgagtagcg agaatatgat ttcgcatggc
     1981 ttgttccgca gaagtcccgt catgtgcaaa taaagcatcc ataatcgctt gatgctcctg
     2041 ccgatctgta tcatcgcggg caggcaaagg gtgcgatgct gtagctgcga agttgccaat
     2101 caacatcgtt gggaaagcag cccacatttg atttagggtg cggattaagt atttatgttt
     2161 actaatcgta aaaatagtct tgtgaaaatt tcggttaagg tcacgatact tcaatacagc
     2221 ttctggagca tcattctctt ccattccaac ctgtatcttt tttagagccg ttaagtcacg
     2281 gtctgtaata atctgtgctg cgaaatacgc tgcacgccct tcaagaaagg cacgatgttc
     2341 atacaggtca attacatctt caatggaaaa ctgaatgaca cgcgcgccgc gataagggac
     2401 atgttcgatc agcccttcat tgacaagctc atttagagct tctcgcacag gcatttggct
     2461 tactcccaac tcctgtgcga ggcgctcctg acgcagccat tctccgggtt tgtattgccc
     2521 atcaagaatg gctgcacgca ttttatctgc gacaacgttc cgtagctgtc tgtgagaatc
     2581 tgaattcata gctccgtctt agtaaatgga tccaccgggt tgggaagatg tcacagtgcc
     2641 attttcagca gtgatcgtgt atgctgttcc gccgctgcct gtccatgttg cgaagttgaa
     2701 tgtggctgag ccgctaatgc gataaacaga aacattacga taggttaggt cggttttagg
     2761 taggcaaact tcaggcgtgc caggagtttg aaggaagtac gcagctccgt ttccaagaat
     2821 agaggcgctc ccgtcagctt caacaactac ggcagttgct tcatcgatgc ccaacccttt
     2881 tgcctgcggt gcccatccat cgttgacgat acgagcaagg aatgtaacga gtcgacccat
     2941 acggtcacgg gtaacaaagt gactatccgt gataagactg ctcaaataag ggacggtaag
     3001 aaaatcacgc tcgagagcaa tacgacgacc aaatgggttg cctaaagccg tggaagagtc
     3061 tactgtgcca tttgctgctg agaaagaaat ttctcccaaa atggcaagcc cggcacttgt
     3121 gcctcctata ggcacgttcc gtgcaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnntctc ccaaaatggc aagcccggca cttgtgcctc ctataggcac gttccgtgca
     3301 atcagattat gaattgcatc ttccactgga gtccctttcc agtagttaac ataatcagcc
     3361 tgatcgcctc ctgcaataaa cagagcttca gcattattga tcttatccaa cacaaagggg
     3421 tcacttgcgc cagtacgtga tgtaataatg agagtctcta cagagtcaac cgtgcctaaa
     3481 ccataaatat aaggattata ggcatctgta cctgaagcgc gaatgacgac aaaatctccc
     3541 ccacctgatt tattgatcat ccattgaaat gctgcatcaa catcggtgcc accgcccatg
     3601 aggaccgttc cggcttgagt aggtgaagtg gcattgtttg agttaccaac aacatagtat
     3661 tcataatgtt tttttgctgc gaaaacgggg ctaggggata taagcgacac caagatgata
     3721 aacaaggtgg tgaatcgaaa aaatggaaat acaagcttca ttttatgctc ctttggcata
     3781 gcacgttatt gaaggtgggt atttctaaat tagatataat atctaggttt aggatagcgt
     3841 ttattggttg atgtgtcaat actttttaag aggaataatt tgatatttat tgactatgcg
     3901 caatttaagg agtgccctta atacatgata atttgactgc ctcattacga atttttgtaa
     3961 ctagcacaaa aaagagaccc tatgcttcag gatctcttta aaatcaatgt ttccctcccc
     4021 cattccatag agtatattta gcgcaagtta ttgtctatgc gcaatttcac tcttgaactg
     4081 taaagtagta tgccacagaa caaaatcttc ttttgccaac cgttaaagta aagtatgtag
     4141 agtaatcacc ggcagctttg ggagcgataa aagatgctcg tacccaaatc tctttgcctg
     4201 gggcaacact ctgggcaaag tcttgaatct ttgtctcttc atggcggaac ccaccggtat
     4261 aaacaaagtc aacaccggtg cgcggccaag actggttacc actattaaac aacatccact
     4321 gaaccgtaaa gtgggtcttt ggtttgaata tcgtattcct aggcggattc aacccatacc
     4381 aaacgcaggt ccaatctttg ccagacatct ttcttgggtc ttcatcttta ttctttggct
     4441 tatcaccatc tgtgttttct tcatcgtcac cagatgcaga ctctggaaca ggagatttca
     4501 aggttccgat cgtaggcgga atggtataag ttggtatcgg tgttggagta gaaagcttaa
     4561 aaataaaggt tggagtagaa ctcggtgtaa ttgacggtgt aaaagtggtg gtgggagttt
     4621 tagtaaaggt aggcatcacc aatgcggttt gagtttgagc cgcttctgcc gtttgaacga
     4681 taaaggtttg aagggaattt gagtcaaatg ttggcaaagg ggcaacagct ggcgcaccgg
     4741 ggacagcaca agccaacatt attaaaatca tgacagcgag aaaaagaggt ttacgcacgg
     4801 gaactcctac ttaaagtata tcgtgaaacc tttttcggat aaactcttct ccctttgcaa
     4861 tgaactcatc atattcgtta ggtacaggcg cggatgccat taaatcctgg gttttttggc
     4921 gcaatacctg acgggccggc ggcgcaccgg catccatcca cttttccatg ctgtagcgcg
     4981 gatagactcc acttacatag tatccgttct tgtaattttt caaggtcgaa ggctggttga
     5041 ggaaactctt gccgggacct actttgaaaa tctcatctac tgcagagttg gcatcgtcta
     5101 attgaaagcc attgtggagg cgtaaggctt ggtctatgat ctcgtgtgcg tggacgaagg
     5161 tggttgggga gatcgacttc gagccaagag aatctccgat aaacggagcc aggtgtccgt
     5221 tagagagaag aagcgcgagg gtattcatcc aataggtgtc tgccgcaata aggtccatgc
     5281 cccagccggt gccactacca gaggtggagc aatgcggaat gccgtaggac ttcatcatct
     5341 cagaacaagc aacactaatg agaatacttt gcgggtcata gaaattgagc atggtgcgca
     5401 tgtcaaaata gacaggcaac atgccgagca aaattggcgc gccttttttg atggtttgac
     5461 taatgaccaa acccgcaagc aattctgcca tcaggagtgt gagcgtgccc gctggcgtta
     5521 atggcgtgga cgcacccgcc atgctgtagt tggaaaagat gatgggaagc ccgcgttcga
     5581 tggcgacttt cattttgtcg acggtgccgg cattcatcac gagcggactg accggattga
     5641 aatacggaat tacaaagggc ttatcgccga cttccttgcc attcaaatgc tcgaacatat
     5701 cgagcacatc tggaaagcgg tcttcatctg aaacaagcaa caccaatggc ttggttgtat
     5761 tggagatatg ctccaatgag ccgtacatat cgccaagttc ttcttttaca tcacggacga
     5821 tccccaccgt tgagatgacg tcataatgtt tgagcccaga agccaagcgt acaaggtcgc
     5881 gaaagttctc acgcttgaaa atatccagcg tttcatccac cgggttttga tagaacagcg
     5941 ccgtcacc
//
LOCUS       DAOREF010000046         5924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7195_length_5924_cov_4.276708, whole genome shotgun sequence.
ACCESSION   DAOREF010000046 DAOREF010000000
VERSION     DAOREF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_5924_cov_4.276708"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4862)
                     /locus_tag="PKI33_01605"
     CDS             complement(<1..4862)
                     /locus_tag="PKI33_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM35722.1"
                     /translation="MPRTGQKKSAAPKSATKKKAVRATTKKTTSSKPTSVTQLRKELE
                     QREAELAIIKSVQDGLASKLEMQAIYDLVGEKIRDLFKAQTTIIATFDRKTETQSFNY
                     YVDRQGREYIEDRPMSGLVKALIREKKTFLFNEHVEDRMREFNAELVLGPLIPKSALY
                     VPLTTAGEVRGVISLQNMDKEHAFNKSDVRLLETLAGSLCAALENARLLNETQRLLNE
                     TEQRNAELEAIRKATINLSSSLDYTTVLNSLLQNTWTLMPSIEDINLFIYENGELKFG
                     TAIAHGKITNAPAFHPRPGGMTDIVSKSGEMMLVEDMSVDPLYANSNWGGALIGLPLK
                     FSNRVVGVMNIHFAEPRIFTESELRLLKLFGDQAAVVIENSRLFNETERLLSETAGRN
                     AELGVINSLQQVLVSKLDFQSIIDLIGDKVGEIFDAQVTLISLYYPATGEMHHRYINE
                     RGQRMYFDEPMPVDGFRKHVLESKQTLLISHDYLRMAEELGEEPASAGEAPKSLLFAP
                     MVVDGQVTGIMSLQNLDREHAFQESDTKLLSTIAASLSAALNNARLFEETMRHARESE
                     ALNEVGRDISATLNLSAVLDKIAKHARDLLKGSSSAIYLPEPDGKSMRALVAQGDIAE
                     EILADSIQVGEGIIGSLAKLGKAEYINDTNHDPRTLQIPGTPAVQNERLLVTPLLSGE
                     KVNGMMAVWRKGGEPFSPADQRFQEELSLQAAIAIQNANFVNEVEQRAHELEIINSIQ
                     QGLSTKLDVQSVYELVGEKVRSIFNSQAVIISYYHPETETADFPYMYWKGERIYPGRE
                     PLSGFSGYVIRNKAPIFINSNLEEMAHSYGSTLLAGDTFPKSLIAIPILSGDQVFGSL
                     SIQNFEQERAFTENDLRLLSTLAASMGISLQNAKLFDESQRLLVETRQRAEELALINT
                     ILSGLDTKLGIQTVYDATGEKIREIFDAQTVVLSIYDKENQLTYYPYIIENGERLYQD
                     PLPLREDGGGFGGYVIRTKEPLLVNSDFEEYSKRFQSANLGVDAEVEVVVRAGLWVPM
                     LVGDEVKGVISLQNLEREDAFSDSDVRLLSTIANSIGVAIENIRLFDETQRLLKETEQ
                     RATELAILNNVSESMSRALDVNAVTHTVGNKIHEIFSAEIVDILLYNSATNIVSLVFS
                     FSNNLSYENEPPWELGEGLTSKIIITKQPLLLNTAKEIEDNGAATYVTAPEDEEDIKS
                     YMGVPIMVGDQVLGVVDVQSYKAHAFDEGSLRILQILSANMGVAIQNARLFDETQRLL
                     KETEQQNSDLSALNSVQQALVSNIDVKSIYQAVGRKLTALFNVHSAVIYTVDLDTYSM
                     TYEYAYEQGKEWDIPPKQATSLHVYITDTVKTNRKSLVINKDFDEFAAQFPDFKTSRS
                     RFPKSLCAVPILIRKNSVTGISLQHLDTEDFFNDTSLRLLETIASATSVALENARLFD
                     ETQRLLNETKRRATELEILNNIGQVLTQQLDVNSIIEMVGDKVRELVNEDNLGIGLYD
                     EETKTVTAHYVTKGSERIQFPPFPINEFTQRAALQGKSLVINRRSPILWKKLGSHMTA
                     MDEIPKSVVMVPMIVGKELIGGFTIQNFEHENAYDESIITLLESIASGTATAIQNARL
                     FEEARLARAAA"
     gene            complement(4976..>5924)
                     /locus_tag="PKI33_01610"
     CDS             complement(4976..>5924)
                     /locus_tag="PKI33_01610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNM35723.1"
                     /translation="AIRNIPIIIISANNDLQSVVKGLQKGAEDYLPKPFEPTILYARI
                     SASLDRKRLRDQEQVYLQSLENELNIARNIQQEFLPAELPVLEGWEIAPYFKAAKFVA
                     GDYYDAFTLPNGNLVFIVGDVCGKGIGAALFMTLFRSLLRATSTAGYLVEETATFSPA
                     KHIEQAIHMTNNYVATTHEQALVFSTVFIGILNPTTGALTYVNAGNEAPFLIRNDGTF
                     EELRPTGPVVGAMPGVAYKVREVLLNSGDSLLAFTDGIPDSKNQQDDFYGHERLVEFI
                     KQKDASATALVNRLGKELDLFIDTEEQFDDITILALRKV"
BASE COUNT         1446 a         1410 c         1455 g         1613 t
ORIGIN      
        1 gccgcggcac gtgccaaacg cgcttcttcg aacaggcgtg cattttgaat ggctgtggct
       61 gtaccagaag caatggactc gagcaaggta atgatggatt cgtcataggc attctcatgc
      121 tcaaagttct ggatggtgaa gccaccgatc agttctttgc ccacgatcat tggcaccatt
      181 acaaccgatt tggggatttc atccatggcg gtcatatggg aaccgagctt cttccacaaa
      241 atgggggagc ggcggttgat gaccagactc ttgccttgca acgcagctct ttgggtgaac
      301 tcgttgatcg ggaacggcgg gaattgaatc cgctcgctgc ctttggttac gtaatgggct
      361 gtgacagtct tcgtttcctc gtcatataaa ccaatgccta aattatcttc attgacgagt
      421 tcccgaactt tgtcgccgac catttcaata atgctgttga cgtcaagttg ttgggtgagc
      481 acttgcccga tgttgttgag aatttccaac tcggtggcac ggcgcttggt ctcgtttaag
      541 aggcgttggg tttcgtcaaa gaggcgtgca ttttcgagtg ccacactggt ggcgctggca
      601 atggtttcta atagacgcag cgaagtgtcg ttgaagaaat cttctgtatc aagatgctgc
      661 aaggaaatcc ctgttaccga atttttgcgg atcaagatcg gtaccgcaca tagcgacttg
      721 gggaaacggc tgcgcgaggt tttgaaatct gggaattgag cggcaaactc atcaaagtct
      781 ttattgatca ccagactctt gcgattggtt tttacagtgt ctgtgatgta cacatgcaaa
      841 ctggtagcct gtttgggcgg aatgtcccat tccttgcctt gttcgtaggc atattcatag
      901 gtcatactat acgtatcaag gtctaccgta tagataaccg ccgaatggac gttgaagagc
      961 gcggttagtt ttcgccctac cgcttgataa atcgacttca cgtcaatgtt ggagaccaat
     1021 gcttgctgta ccgagttgag agccgaaagg tcggaattct gctgttcggt ttcttttaag
     1081 aggcgctggg tttcatcgaa taagcgtgca ttttgaatgg caacgcccat attggcagaa
     1141 aggatttgta gaatgcgcaa actgccttcg tcgaaggcat gagctttata gctttggaca
     1201 tctacaacgc ctaatacttg gtcgccaacc ataatgggca cacccatata agacttaata
     1261 tcctcttcat cttcaggagc ggtgacatag gtcgctgcac cgttgtcttc gatttctttg
     1321 gcagtgttta acaacaacgg ctgctttgtg ataatgatct tggaggtgag cccttctccc
     1381 aattcccaag gtggttcatt ctcataagaa agattgttgc taaagctgaa taccaaactc
     1441 acgatattcg tggctgagtt ataaagcaga atgtccacga tctcggcact gaagatctcg
     1501 tggatcttgt tgcccacggt gtgagtgacg gcgttgacat ccaacgcgcg cgacatcgac
     1561 tcgctgacat tgttgagaat ggcaagttcg gtggcgcgct gctcggtctc tttgagcaag
     1621 cgctgagttt cgtcgaagag gcgaatgttc tcaatcgcca ccccgatcga gttggcaatc
     1681 gttgaaagca agcgcacatc tgagtcgcta aaggcatctt ctcgctcaag gttttgcagc
     1741 gagatcacgc ctttcacttc gtcgccgacc aacataggca cccacagtcc ggcacgcacc
     1801 actacttcta cttcggcatc cacaccaagg tttgccgatt ggaagcgttt ggagtattct
     1861 tcgaaatcgc tgttgaccaa tagcggctct ttggtgcgga taacgtatcc accgaacccg
     1921 cctccatctt cgcgaagcgg taaggggtct tgatagagcc gttcaccatt ctcgataatg
     1981 tatggataat aggtgagttg attttcttta tcgtagatgc taagcacaac ggtctgtgca
     2041 tcgaagatct ctcgtatttt ttcaccagtt gcgtcgtata ccgtttgaat gccaagtttg
     2101 gtatccaacc cgcttaagat ggtgttgatc aacgccaatt cttctgcgcg ttgacgggtt
     2161 tcgaccaaca atctttgcga ctcatcgaat agcttggcat tctgaagcga aatccccatg
     2221 cttgctgcaa gggtcgaaag caaacgcagg tcattttctg taaaggcgcg ctcctgttca
     2281 aagttctgaa tggaaagcga accgaagacc tggtcaccgg agagaatggg aatagcgatc
     2341 aacgattttg gaaaggtatc cccagccaaa agagtagacc cgtaactgtg tgccatttct
     2401 tccaagttcg agttgataaa gatgggcgct ttgttacgaa tgacatagcc agagaaacca
     2461 gaaagcggct cacgcccagg gtagatgcgt tcccccttcc aatacatgta agggaaatcg
     2521 gcagtttctg tttcggggtg ataatacgag atgatcacag cttgcgagtt gaagatgctg
     2581 cgaactttct ctccaaccag ttcataaacc gactgtacat ctaatttggt cgataaccct
     2641 tgttggatgc tattgataat ctccaattcg tgggcacgtt gttccacttc attgacgaag
     2701 ttcgcatttt gaatggcaat cgcagcttgc agagacaact cttcttgaaa acgctggtct
     2761 gcaggggaga aaggttcgcc gcctttgcgc caaaccgcca tcataccgtt gaccttctca
     2821 ccagaaagca gcggagtgac caacaagcgt tcattttgaa ccgcaggtgt gccagggatt
     2881 tgtaacgtgc gcgggtcgtg attggtatcg ttgatgtatt ctgccttacc gagtttggca
     2941 agcgacccaa taatgccttc cccaacctga atggaatctg ccagaatttc ttctgcaata
     3001 tctccttgcg ctaccaacgc ccgcatcgac ttgccatctg gctcgggcaa ataaatagca
     3061 ctgcttgagc ctttcaataa gtcgcgggcg tgcttggcga tcttatctaa cacggctgag
     3121 agatttaacg tggcagatat atctcgccca acttcattga gcgcttctga ttcgcgggca
     3181 tgccgcatgg tttcttcgaa taagcgggca ttattcaacg ccgcacttaa gctagcggca
     3241 atggtagaaa gtaactttgt atccgattct tgaaaggcgt gttcacggtc caggttttgc
     3301 aagctcataa tgcctgtcac ctgaccatcc actaccatgg gagcaaagag cagcgacttt
     3361 ggtgcttcac ctgcagaagc aggctcttca ccaagttctt ccgccattct taagtaatcg
     3421 tggctaataa gcagggtttg tttgctttca agcacatgct tgcggaaacc atccacgggc
     3481 atgggttcat caaagtacat gcgttgaccg cgttcattga tatagcggtg atgcatctca
     3541 cctgtggcgg gataatacaa tgagatgagt gtgacttgcg catcaaagat ctcgccgacc
     3601 ttatcaccga tcaggtcaat gatggattga aaatccagtt tagagaccag cacttgttgc
     3661 aggctgttga tgacgcctaa ctcggcattg cgcccggcag tttcagataa gaggcgctcg
     3721 gtctcattga acaaacgcga attttcgatg acgactgcgg cttgatctcc gaacaatttg
     3781 agtaggcgta attcagactc tgtaaaaata cgcggttctg caaaatggat attcatcaca
     3841 cccacgacac ggttgctgaa tttgagcggc aacccgatca atgcgccacc ccagttggag
     3901 ttggcgtaga gtgggtcaac gctcatatct tctaccaaca tcatttcgcc agatttcgaa
     3961 acgatatcgg tcataccgcc cgggcgcgga tggaaagctg gcgcatttgt aatctttcca
     4021 tgcgcaatgg ctgtgccaaa ctttaattcg ccgttctcat agataaaaag attgatgtcc
     4081 tcaatgctgg gcatcaatgt ccatgtattt tgtagcaagg aattgagaac ggtagtgtaa
     4141 tctaaacttg aagagagatt gatggtcgct ttgcggatgg cttccaactc cgcgttgcgt
     4201 tgttctgttt cgttgagcag acgttgcgtt tcatttaaga ggcgggcatt ttcgagcgca
     4261 gcacacagag atccggcaag agtctcaagc aagcgcacat ctgatttgtt gaaggcgtgc
     4321 tccttatcca tattttggag ggagatcaca ccgcgcacct caccggctgt cgtcaacggc
     4381 acatacaaag ccgactttgg aatcaacgga ccgagaacca actctgcgtt gaactctcgc
     4441 attcggtctt caacatgttc attaaagagg aaagtctttt tctcgcggat caaggctttg
     4501 accaacccgc tcatggggcg gtcttcaata tattctcgtc cctgccggtc tacataatag
     4561 ttgaacgatt gcgtttctgt cttgcgatca aaggttgcaa tgatggtggt ttgcgcctta
     4621 aaaaggtcgc gaatcttttc cccaactagg tcataaatcg cctgcatctc caatttggag
     4681 gcaagcccat cttgcacact tttgataatg gcaagctccg cctcacgttg ttccagctcc
     4741 ttgcggagtt gggtaaccga agtgggtttg ctgctggttg ttttcttggt tgtagcgcgg
     4801 acggcttttt tctttgtggc tgatttgggc gcggcgcttt tcttctgacc agttctaggc
     4861 atggtttcct ctgagaatga ttcagtttac atagggggca gagaatcgtc aatagcgtag
     4921 ataaaaaaac aggcggagaa cgcctgtctg tcattcaatt gttatgttca gacaatcata
     4981 ctttgcgcaa cgccaggatg gtgatatcgt cgaattgttc ttcggtatca atgaacaagt
     5041 ccaactcttt gccaagacgg tttacaagcg ctgttgcaga ggcatccttt tgtttgatga
     5101 attccaccag gcgttcatga ccataaaaat catcttgctg gtttttgcta tctggaatgc
     5161 catcggtgaa agcgagcaag ctatctcctg aattaagtaa aacttcacgc actttgtatg
     5221 ccacacccgg catcgcaccc acaaccggac ccgtcgggcg cagttcttca aacgtcccat
     5281 cgttgcggat taagaacggc gcttcgttcc cggcgttgac ataggtcaac gctccagtgg
     5341 ttgggttcaa gatgccgata aaaactgtcg aaaagaccaa ggcttgttca tgcgtggtcg
     5401 ccacataatt gttggtcatg tgaatggctt gttcaatatg tttggcaggg gagaaggtgg
     5461 cagtttcttc tactaaatat cccgccgtag atgtagcacg caacaaacta cggaacaagg
     5521 tcatgaacaa tgcggctcca atacctttcc cgcaaacatc tcctactata aagaccaaat
     5581 ttccattagg taatgtaaag gcatcgtaat aatcgccagc cacgaacttt gccgctttga
     5641 agtacggagc gatctcccaa ccttccaaca cgggcaactc ggctggaaga aattcctgct
     5701 gaatgttgcg cgcaatgttc aactcgtttt caaggctttg taaatagact tgttcttggt
     5761 cacgcaggcg tttcctatcc aaactggcgg aaatgcgtgc atataaaatg gtcggttcga
     5821 aaggcttggg taaataatct tcggcacctt tttgaagccc tttcaccacg ctttgcagat
     5881 cgttgttcgc agaaataata atgattggga tattacgaat cgcc
//
LOCUS       DAOREF010000047         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7203_length_5920_cov_5.295482, whole genome shotgun sequence.
ACCESSION   DAOREF010000047 DAOREF010000000
VERSION     DAOREF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7203_length_5920_cov_5.295482"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKI33_01615"
     CDS             complement(<1..186)
                     /locus_tag="PKI33_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35724.1"
                     /translation="MTPLHPLAGVYAAAVTPYLGAPKTAGKPDTAFDFESLAMFLHFL
                     AGRGCHGCRPRAHPAGGR"
     gene            complement(259..1107)
                     /locus_tag="PKI33_01620"
     CDS             complement(259..1107)
                     /locus_tag="PKI33_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35725.1"
                     /translation="MSDVIDSKIRADFSRARFKSFINQVFSVLSGQRNTLLSYDDIKE
                     KLRIGGPIYRGMKTVRVEQIAGSLNRYHEFDRAFLPKEDQLASRWQKVDRAFYEDIHL
                     PPVVLYKVGQVYFVVDGHHRVSVAREQGQEYIEAEVRECATRVNITPDIRPEDLEVLG
                     AKVDFLERTGLDKYRPDANIKLTIPDGFTRMLEHIAVHRYFMGLDFKRDISEQEAVEH
                     WYDKVYVPIVSIIRESGLLDEFPDKTESDLYLWALDHQHYLYQEEGQPLQPPELAAKQ
                     FILENE"
     gene            complement(1202..1825)
                     /locus_tag="PKI33_01625"
     CDS             complement(1202..1825)
                     /locus_tag="PKI33_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNM35726.1"
                     /translation="MKILAVSDQVVERVYSLAPSGHFKGVELILGCGDMPYTYLEYLV
                     TILSVPLFYVPGNHDPDFNPRDHRSRAEGGSNLDLRLVYHNKSLIGGFGGSIRYRPDG
                     TNQYTQNEAFLRAFNMVPKLVMNRIKYGRALDILISHSPPFGIHDDTDYAHNGLKALN
                     WLLDFAKPRYHFHGHTHFYRNNIEQSITKTSTTQIMNVYPYKVVDIE"
     gene            1950..3857
                     /locus_tag="PKI33_01630"
     CDS             1950..3857
                     /locus_tag="PKI33_01630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM35727.1"
                     /translation="METRNITSPIGPYGSGSGSGSGDQAGRQQLIPGVTLVNRYSIQD
                     VIGVGGMGSVYRARDMHFPNVVKLVAVKEMINMAPDPLVRQTIVQNFEREANLLATLH
                     HPSIPRIYDYFSHDDRSYLVLEFVQGRDLETVINEANGFLTEDQVISWGIQLCDVLEY
                     LHGHKPDPIIFRDMKPSNVMINQNGDVILVDFGIAKTFQTGIKGTMIGTEGYSPPEQY
                     RGDATPLADVYALGATMHHSLTRRDPRLEPPFTFAERPIRRINSNVSIELEAVVNTAL
                     NYNPTERFPTAASMKEALMNVARKTGTLSKLSAVLPTPASSAIKPIWDFKCEDEIRGT
                     PTLSQGSLYIGCYDNNLYSLNAADGKFQWKYPTDGGIVSRPLVFENNVFFCSEDQRLH
                     VIGARTGKLLWTYYTEGKIYSSPRISDGHVFFGSEDHDLHAVNVNSGRAVWKFSTDGP
                     IYSTPLVANEMIYFGAESGSFYGLDFRGAMKWRFAAKRGMTSSPISKGQAIYTASLDG
                     TLYALDAKSGWAIWKFRLGKGSVSSPVIADDFIFLGAADGFVYCVDIRTAKEVWRYKT
                     DNQVSSSPTIYKDSIYIGSVDGSMYCLEYRTGRLRWKFETGGPITGSPVVLDDIVYFG
                     STDHHVYALFA"
     gene            3869..4780
                     /locus_tag="PKI33_01635"
     CDS             3869..4780
                     /locus_tag="PKI33_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNM35728.1"
                     /translation="MADFFKKLFGSKEESTKPRQVDSATTAPLSDQQLQSILSSQAPR
                     YEIKQLVAAVGQSVGKQRDHNEDSVLSLTNTISGSSESVPFGLYIVADGMGGHQFGEV
                     ASNAAIRIVGGNITKKFHSYLYKLPTQSLQESLQEVMESSVMEAHQYIQREAPGSGTT
                     ITAALVLGQQVTIGHVGDSRAYAVYPDGRMDPLTRDHSLVKRLEELGHLSKDEVENFP
                     HRNVLIRALGQGEVLEADVFTVPFPQSGYLMICSDGLWGVVNERDVARIINEAPNLQR
                     ACQSMVEAANAAGGPDNITVILAQLIG"
     gene            4789..>5920
                     /locus_tag="PKI33_01640"
     CDS             4789..>5920
                     /locus_tag="PKI33_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNM35729.1"
                     /translation="MSASKPDLYSVLGVLRDATQEEIKHAYLAAAQRLHPDKNVAAGE
                     TELFLDIQQAYEVLSNPKRRSQYDSTLPPEVEEKNQFIRHEVLFSRPSLVHLKEDQLL
                     YVLLEVEPTQKQSASLPTPPLNLCLVLDRSTSMQGDKMDILKSTAIQLIRSLRPQDVL
                     SIVTFSDRAEVIVPASISLDKRKQEGNVQMIRPSGGTEIFQGLDAGFKEVRRTLDPAR
                     VNHIILLTDGNTYGDEQDCLQLAEQAAALNIGITGMGIGHEWNDIFLDALAAKTGGSS
                     VYISQPKDIERFLIEKFKALTSVYADEVSLDIRELEHIRLNFAFRIQPEGSQIATTSP
                     LRMGPILRDVPLHVLLEFVVSPEALKGEKALVLDGLLKASIAS"
BASE COUNT         1545 a         1338 c         1381 g         1656 t
ORIGIN      
        1 tcttccgccc gctggatgtg cccgagggcg gcagccgtga catccgcgcc cggcgaggaa
       61 gtgaagaaac atcgccagtg actcaaagtc aaaagccgtg tctggtttcc cggctgtttt
      121 aggtgcaccg aggtaggggg taactgcagc agcataaaca cctgctaatg gatgaagggg
      181 tgtcatagat gaactttgtc tccaaaataa agtagggacg acctttcaaa atctttcttt
      241 ggggtcgtcc ctactagttt actcgttctc gagaataaat tgtttagcgg ctaattccgg
      301 cggttgaagt ggttgccctt cttcctgata tagataatgc tgatggtcta acgcccagag
      361 atataggtcg ctttcagtct tatctggaaa ttcatccaag agtccgcttt cacgaataat
      421 ggatacgata gggacgtaca ccttgtcgta ccaatgttcc acagcttctt gttctgaaat
      481 atcgcgtttg aaatcaagcc ccataaaata acgatggacg gcgatatgtt caagcatacg
      541 tgtgaaccca tcagggatcg tcagtttgat atttgcatcg ggtcggtatt tgtccaaacc
      601 ggtacgctca agaaaatcca ctttggcacc gagaacttca aggtcttcag gacgaatatc
      661 tggcgtgatg ttgactcgag ttgcacattc acgcacttca gcttcgatat attcctgtcc
      721 ttgctcacga gcaactgaca cacgatgatg tccatccaca acgaagtaca cctgacccac
      781 tttatataga accacaggcg gtaagtgaat atcttcataa aaagcacgat cgactttttg
      841 ccagcggctg gcaagttggt cttccttggg caagaaggct cggtcgaatt catggtaccg
      901 gttcagactc cccgcaatct gttccacgcg gacagttttc ataccccgat agatgggtcc
      961 cccaatacgg agtttttcct ttatatcatc ataagacagc aaagtatttc gctgcccact
     1021 taagacagag aatacctgat tgatgaagga tttgaaacgt gcccgactaa aatctgcacg
     1081 tatttttgaa tctattacgt ctgacatttc tttctctctt tgctaataac aattcagtct
     1141 attatataaa acatactaat aaataagtta ttgtgagatt aatatccgat taggatatgt
     1201 cttattcaat atcgacaacc ttgtaaggat agacgttcat gatctgagta gtgctagttt
     1261 ttgttatgct ctgctcgata ttattccgat aaaagtgcgt atgaccatga aaatgatagc
     1321 gtggttttgc aaaatctaac agccaattta atgcttttaa cccattatgg gcataatctg
     1381 tatcatcatg gatcccaaat gggggagaat ggctaatgag aatatccagt gcgcgcccat
     1441 acttgatacg attcatgacc aatttgggta ccatattgaa tgcccgtaag aaagcttcat
     1501 tctgtgtata ttgattcgtg ccatcgggtc tgtagcggat ggaaccgcca aagccgccga
     1561 tcaagctctt gttgtggtag accagtctca agtctagatt tgagccgcct tctgctcgag
     1621 aacggtggtc acgcgggttg aagtctgggt catgattccc cggaacataa aaaagtggca
     1681 cactcaaaat agtgacgagg tattcaaggt aggtgtaggg catgtctcca cacccaagaa
     1741 tcagttcaac acctttgaaa tgtccgctcg gcgccagcga gtagacacgc tctaccacct
     1801 gatcacttac tgccaatatc ttcacagggg ctcattgtgc cgtagaaaac gctcgattgc
     1861 aaccccaaaa gtgagctttt tgcaaaattg caatagccag catcggcaaa tacccttata
     1921 attcattaaa tttttggaaa aaaacacaga tggaaacgag aaacattacc tctcccattg
     1981 gtccttatgg ctcgggttcc ggctcaggct ccggtgatca agccggacgt cagcaattaa
     2041 taccaggcgt gacccttgtc aaccgctata gcattcaaga tgttataggc gttggtggta
     2101 tggggtctgt gtatcgcgct cgggatatgc atttcccgaa tgtggttaag ctagtagctg
     2161 tcaaagaaat gatcaatatg gcaccagatc ccttggttcg ccagacgatc gtacagaact
     2221 ttgagcgtga agccaatttg ttggctactc tgcatcatcc atctattcca cgtatttatg
     2281 attatttctc ccatgatgat cgttcttatc ttgttttaga gtttgttcaa ggtagagacc
     2341 ttgaaacggt tatcaatgaa gcaaatggct ttctgacaga agatcaggta attagctggg
     2401 gaatccagct ttgtgatgtg ttggaatatc tgcacggtca taagccagat cccatcattt
     2461 ttcgtgatat gaaaccctcg aacgtgatga tcaaccagaa tggtgatgtc attttggttg
     2521 acttcggtat cgccaagaca ttccagaccg gtattaaagg tacgatgatc ggaacggaag
     2581 gatattcgcc acctgagcaa tatcgtgggg atgccacgcc tcttgcggat gtgtacgcgc
     2641 tgggtgccac catgcatcat tctcttaccc gacgcgaccc acgcctcgaa cctcctttta
     2701 cttttgccga acgcccaatc cgccgcatta actcgaatgt ttccattgag ttagaagctg
     2761 tggtaaatac ggcgttgaat tacaacccta ctgaacgctt cccaacagca gcatctatga
     2821 aggaagcttt gatgaatgtg gctcgaaaga caggaaccct ttcaaagctt tctgcggttt
     2881 tgccgactcc tgccagtagt gcgatcaagc ctatttggga ttttaagtgt gaagatgaaa
     2941 ttcgcggcac accaacattg agtcagggat ctttgtatat tggctgttat gacaataacc
     3001 tttattccct gaacgctgct gatggaaagt tccagtggaa atacccgact gatggcggca
     3061 tcgtttcccg cccattggtt tttgagaata atgtcttctt ttgttctgaa gatcaacgct
     3121 tgcatgtaat tggtgcccgc acaggcaaat tactgtggac ttattacaca gaaggaaaga
     3181 tttattcttc tccacgcatc tcagacgggc atgtcttttt cggctccgaa gatcatgacc
     3241 ttcacgctgt gaacgtgaat agtggtagag cggtatggaa gttttctacc gatggaccta
     3301 tttactctac tccgcttgtg gcaaatgaaa tgatctactt tggcgcagag tctggttcgt
     3361 tttacgggct agatttccgt ggtgccatga agtggcgttt tgccgctaaa cgtggcatga
     3421 cgtcttcacc aatcagtaag ggacaggcaa tttatacagc ctctttggat ggaaccttgt
     3481 atgccctcga cgcaaaaagc ggctgggcaa tctggaaatt ccgtttaggg aaggggtcag
     3541 tctcttcgcc ggttattgcg gacgatttta tcttcctggg tgcagcggat ggctttgtat
     3601 actgtgttga tattcgcact gccaaagaag tttggcgcta taaaacggat aatcaggtga
     3661 gcagttcacc aaccatctat aaagactcta tctacatagg ctctgtggat ggttccatgt
     3721 actgtcttga atatcgtaca gggcgtttac gttggaaatt tgaaacaggt ggacccatca
     3781 ccggttcccc cgttgtttta gatgatattg tttattttgg ctcaaccgac catcacgttt
     3841 atgcgttgtt tgcctaaaag gagaacttgt ggctgatttt ttcaagaaac tttttggttc
     3901 caaagaggaa tcaacaaaac caagacaagt ggattctgct acaacagctc cgctttctga
     3961 ccaacaactc cagtccattt tatccagcca ggcacctcgc tatgagatca agcaacttgt
     4021 tgcggctgta ggtcagtctg tagggaaaca acgtgaccac aatgaggata gtgtcctttc
     4081 cctgacgaac accatttcag gtagttctga gagtgtacca tttggcttat atatcgttgc
     4141 cgatggtatg ggtggacatc aatttggtga agtagcaagc aatgctgcta ttcgcattgt
     4201 cggtggcaat atcactaaga aatttcattc ttacttgtat aaacttccaa ctcaatcatt
     4261 gcaggaatcc ttgcaagaag tgatggagtc ctctgtgatg gaagctcatc agtatattca
     4321 acgggaagcc cccggtagtg gtactaccat cacagctgcc cttgtacttg gtcagcaagt
     4381 tacgattggg catgtggggg atagccgtgc atatgcggtt tacccagatg gcagaatgga
     4441 tccattgacc cgtgaccact ctttggtcaa acgtcttgaa gaactcgggc atttgagcaa
     4501 agatgaagtg gaaaactttc cgcatcggaa tgttttgatc cgtgccttgg gtcagggtga
     4561 agtacttgaa gcagatgtct ttacggttcc tttcccgcaa tcgggatatc ttatgatttg
     4621 cagcgatgga ctttggggtg ttgtaaacga gagagacgtt gcccgtatta ttaatgaagc
     4681 cccaaatttg caacgggcat gtcagagcat ggtagaagct gccaatgcgg ctggaggacc
     4741 ggataatatc acggtcattc tcgctcaact tattggataa cccctctcat gtctgccagc
     4801 aagccggatc tatactccgt tctaggtgtg ttacgtgatg ccacacagga ggagattaag
     4861 catgcctatt tggctgccgc tcaacggctg catccagata aaaacgtagc ggctggcgag
     4921 actgaactct tccttgatat tcaacaggcg tatgaagttc tctcgaaccc caaacgccgc
     4981 agtcaatacg actctacgct cccacctgaa gttgaagaaa aaaatcaatt tattcgtcac
     5041 gaggtccttt tcagccgtcc tagtttggtc cacttaaaag aggaccaatt attgtatgtg
     5101 ctgttggaag ttgaacctac acagaagcaa tctgccagcc tacctacgcc tccattaaac
     5161 ctttgcttgg tgttagaccg atctacctcc atgcaaggcg ataagatgga tatcttgaag
     5221 tctacagcaa ttcagctaat ccgtagttta agaccgcagg atgtgctaag catcgtcacc
     5281 tttagtgacc gtgctgaagt tatcgtccct gcttcgatca gcctagacaa acgcaaacag
     5341 gaaggcaatg tgcaaatgat ccgtccttct ggtgggacgg aaatctttca ggggttagat
     5401 gccgggttca aagaagttcg ccgaactcta gacccagcac gtgtcaatca catcattctt
     5461 cttaccgatg gaaacactta cggcgatgag caagattgtt tgcaacttgc ggaacaagct
     5521 gctgccctga atattggtat taccggtatg ggaattgggc atgaatggaa tgacatcttt
     5581 ttagatgccc ttgccgcaaa aactggcggg tcttctgttt atatatccca gccgaaggat
     5641 attgaacgtt tcctgattga aaaattcaaa gctctgacca gtgtttacgc cgatgaagtg
     5701 tcactggata tcagagagct tgagcatatt cgccttaatt ttgcttttcg catccaacca
     5761 gagggcagtc aaattgcaac cacctcgcca ttacgaatgg gaccgatttt gcgcgatgtg
     5821 cctttgcacg ttttgttgga atttgttgtc agccccgagg cattgaaagg ggagaaggct
     5881 ttagtattgg atgggctcct aaaagcatct attgcttcca
//
LOCUS       DAOREF010000048         5911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7228_length_5911_cov_5.375171, whole genome shotgun sequence.
ACCESSION   DAOREF010000048 DAOREF010000000
VERSION     DAOREF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7228_length_5911_cov_5.375171"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..395)
                     /locus_tag="PKI33_01645"
     CDS             complement(21..395)
                     /locus_tag="PKI33_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35730.1"
                     /translation="MSTCALSVSDDKKFIIMKITGQVNSQDMYQHTLEAHQLGRSLHL
                     RAFLVDLTDAINTQSVVEKYDFVYKKVQEDDELLHFVRMAMWVKKGDHSHDFIETLAN
                     NTGEQAKIFHDQQAAIRFLLEP"
     gene            742..4803
                     /locus_tag="PKI33_01650"
     CDS             742..4803
                     /locus_tag="PKI33_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM35731.1"
                     /translation="MKFKPISSPLRTALIYLIFGGLWILSSDTVLGLFIHHASNLYPL
                     AQTLKGWLFVLVGAVLLYSILSVDMKALHKREEEIRQSHNLYQQLFDSNPQPMWVYDL
                     DTLRFLMVNHAALEHYGYTETEFLSMTIKDIRPPEQLAALTANLAQPPQPVERSVGWV
                     HRKKDGSLIDVEISSHAIDFKGRAARMVLANDVTERKKIESALQASEAQFRNLVERTP
                     AITYISTLDEISTTTYISPQIEAVLGFSPKEFEATPHLWEQQLHPEDHQYTIQTFQES
                     QKEGRSFICEYRMISKQGEVVWFRDQANLVRNENGKPLFYEGFMLVITEQKLAEQALQ
                     ASEIRFRALFEDVPIAIWEEDFSAVKEHIDLLKTQGVTDFRAYLTAHPNRVHEFMEKA
                     KVLNVNRTALNLYKAASKEELIASTTAELSPGEAENNIEDLIAIAEGRLGNHWEGADT
                     TLKGEKLQINLQWSVVPGHETDYSKVIVTTVDITERKQAEEALRVSEENYRGLVESSD
                     NAIAVFDRDGNVLYANQSSQQLWNAPELVGKTLYDLFPKDFADHHLISMKKVIDEQII
                     DTNELEVVIHGNKMWFHVTMSPLKNSAGSVDRLLLNALNLTERKRAEEAQRRSEILFF
                     KIFRASSIGIHLFRLSDGRSYNVNDAFLEIIGYPREELIGRTAVELNLFEDIETRNRW
                     MQALREGRSVRNQDARIRRKSGEIRDCLASLDLIDVSGEVMVLVIATDITERKRAEVQ
                     LRQSEERYRTTLDSMLEGIQIIDSDWRYTYINEAAAKQGQSSPEQLLGRTMMEAYPGI
                     EHTELFSILRDCMTHRTVKRFENKFIFPDGFSGWFELNVQPAPEGIFILSVDVTERKK
                     AEEELRGSEERFRLMAENIEEGFWITNPNSDEEIYLSPAVERIFGRSMNDLMARSSSF
                     IESILPEDVPLVLTNMERQKLGTATNMEYRIKRPDGRIRWVWDRAFPILNPNGTVRLV
                     TGITTDITERKATDAKIQTQIKRLSALNSIDRAISSSMRMDVALDVLLIEVVAQLNVD
                     AASILLLNETDQTLDFAAGKGFNTSIEHASVPLGFELAGKVGTERKVIHVPNLSELKK
                     GVKRKELIQEEHFLEYFGVPLIVKGQLKGVLEIFNREALNPDPEWLTYLETLGGQAAI
                     AIESAQLFEGLQRSNQELFMAYDATIAGWSHAMDLRDKETEGHTQRVTEITVRLAERM
                     GISQQELVHIRRGALLHDIGKLGVPDHILLKPDKLTDEEWHIMRMHPVYARDMLTPIH
                     YLRPSLDIPYCHHEKWDGTGYPRGLKGEDIPLAARLFAIIDVWDALRSDRPYRAGWSA
                     EKTRDYIQSESGKHFDPKVVEYFLALLEELSKS"
     gene            5003..>5911
                     /locus_tag="PKI33_01655"
     CDS             5003..>5911
                     /locus_tag="PKI33_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM35732.1"
                     /translation="MTAISKAMPKNKKGETGQKRQIKTSRANSPSAHHHIMNEVNLDI
                     NNKSKTAIQRLKAENARLQADLETLMRERRTMAETSLRHSQELDLLEKVRATIATKLE
                     LPALLHSVVESIVEICGYAYVSIYLLEGETLILQHQAGYQKVIEKINIHEGISGRVAR
                     TGQPILIEDAKGEPEFLQASKKIRSEITVPLMDNGQVTGTLSIESTSKLTDADLRIMM
                     ALGAEINVAIERSRLYEDLQKRNRTLSALQEATLTIIGKLGLPDAVQTILAQTAQLLN
                     TSHGYVYLVQPNGTLQMATGLGVYPKY"
BASE COUNT         1702 a         1440 c         1359 g         1410 t
ORIGIN      
        1 tttgatggat ggagcacgtt ctacggttca agtaaaaagc ggatagccgc ttgttggtcg
       61 tgaaatattt tggcttgctc ccctgtgttg ttggcaaggg tttcgataaa atcatgggaa
      121 tggtcgccct ttttcaccca catcgccata cgaacgaaat gaagcaactc gtcatcctcc
      181 tgaacttttt tatagacaaa atcatatttt tcgactacgg attgggtatt gatggcatcg
      241 gtcaggtcga cgagaaaggc gcgcagatga agggaacgcc ccaattgatg cgcctccaat
      301 gtatgctggt acatgtcctg actattgacc tgcccggtaa ttttcatgat gatgaatttt
      361 ttatcatctg aaacagaaag cgcacaagtg ctcatagtgg tctcctatcc ctattaagac
      421 ctacttccat tgtattttat atttggctac gtttcaatca attttctcta tgacttgtat
      481 acattcctag ctttacattg taacctactt tgccaattgt tcaaattttg gtaaaaccag
      541 ctccaaacca aaagagacag tcccgtagca ggggctgtct cttgctttaa gggtggaata
      601 ggaggtcaaa tcgtcaacag gaggtcaact tacactataa tcttgttact tagtaacaat
      661 gtcaggcaaa gcctgaagtg ctatactttt tttattggtt aacttttaga ggtttaacat
      721 tttgttgcat ggaggtagtt tgtgaagttc aagcctatct caagcccact acgaaccgca
      781 ctgatctacc tgatctttgg gggactatgg attctttcca gtgataccgt attaggtctt
      841 tttatccatc acgcatcgaa tctctaccca cttgcacaaa ccctcaaggg ctggcttttc
      901 gtgctggtcg gtgccgtgct cttgtattcg atcttatctg tagatatgaa agccctacac
      961 aagcgcgaag aggaaatcag gcaaagccac aacctatatc aacagttatt cgatagcaac
     1021 ccacagccca tgtgggtcta tgatcttgat actctgcgct tcttaatggt caatcacgcc
     1081 gcgttagagc actatggcta tacagaaact gaattcctga gcatgaccat caaggatatt
     1141 cgtccgccag agcagttagc tgccctcacg gcaaatctgg cacagccgcc ccaacccgtg
     1201 gaaagatccg ttggctgggt gcaccgcaag aaagacggct ccttgatcga tgtggaaatc
     1261 tcctcgcacg ccatcgactt taaaggcaga gcggcccgta tggtgctggc aaatgatgtc
     1321 accgaacgaa aaaagatcga atccgccctg caagcctcag aagcccagtt tcgtaatctg
     1381 gtcgaacgca cgcccgcaat cacctacatt tccaccctcg acgaaatcag caccaccaca
     1441 tacattagcc cacagatcga agccgtattg gggttctctc ccaaagaatt tgaagcgacc
     1501 ccgcatttat gggagcagca actccacccg gaagatcatc aatatacaat tcagaccttt
     1561 caagagagtc aaaaagaggg gcgttctttt atctgtgaat atcgcatgat cagcaagcaa
     1621 ggcgaggtcg tttggttccg agaccaagcc aacttggtca ggaatgaaaa tggaaagcca
     1681 ctcttttatg agggctttat gttggtcatc accgaacaaa agctggcaga acaagcccta
     1741 caggcgagtg aaatccgctt ccgcgccttg tttgaagatg tgcccattgc catttgggaa
     1801 gaagattttt cagcagttaa ggaacacatc gatcttctaa aaacacaagg ggttactgat
     1861 ttccgagcct atttaacagc ccaccctaac agggtgcatg agtttatgga aaaggcaaag
     1921 gtgttgaacg tcaaccgcac cgcgcttaac ctgtataagg cagccagcaa agaggagtta
     1981 atcgccagca caaccgcaga actcagcccc ggcgaagcgg aaaataatat tgaagacctc
     2041 attgccattg ccgaaggcag gcttggcaat cactgggaag gagccgatac caccctaaaa
     2101 ggcgaaaagc tccagatcaa cctgcaatgg tctgtagtgc cggggcatga aaccgactat
     2161 tccaaagtga tcgtcaccac cgttgatatc actgaacgca aacaagctga agaagccttg
     2221 cgcgtgagtg aagaaaatta tcgcgggttg gtagagtctt ctgataacgc gattgccgtg
     2281 tttgatcgag acggcaatgt gctttatgca aaccaatcca gccagcaatt atggaacgcc
     2341 cccgaactgg tcggcaagac cctgtacgac ctcttcccga aagactttgc agaccaccat
     2401 ctcatttcca tgaaaaaggt catcgatgaa cagatcatcg acaccaatga actggaagtc
     2461 gtgatccatg gaaacaagat gtggttccat gtcaccatga gtccgctaaa aaattcagcc
     2521 ggctctgtgg acaggctgtt gttaaacgcg ctcaacctca ccgagcgcaa aagagccgag
     2581 gaagcgcaac gccgatcaga gattctattc tttaagatct tccgtgccag ttccatcggc
     2641 atccatctct tccgtctttc cgatgggcgc tcctataatg tcaatgatgc gtttcttgag
     2701 atcatcggtt accctcgcga agaattgatc gggcggactg ccgtagagtt aaatctgttc
     2761 gaagatattg agacgcgaaa taggtggatg caagccctac gagaaggcag gagcgtccgc
     2821 aatcaagatg ccagaatcag gcgcaaatct ggcgagattc gagactgcct cgcatcgctg
     2881 gacttgatcg atgtcagcgg cgaggtcatg gtgctggtga ttgccaccga cattaccgaa
     2941 cgtaaacgcg ccgaagtcca attaagacaa agcgaagaac gctaccgcac cactctggat
     3001 agtatgttgg aagggattca gatcatcgac tcggattggc gctacaccta catcaatgaa
     3061 gctgccgcca agcaagggca aagctcgcct gaacaactcc tggggcgcac catgatggaa
     3121 gcgtaccccg gcattgagca cacggaactt ttttccattc tgcgtgactg catgacccac
     3181 cgcacggtca aacgctttga aaacaaattc atcttcccag atgggtttag tggttggttt
     3241 gaactcaatg tgcaacctgc cccagaaggc atttttatcc tttcggtaga tgtcacagag
     3301 cgtaagaaag ctgaagaaga actgcgaggg tcagaagaac gcttccgctt gatggctgaa
     3361 aacattgaag aaggtttttg gatcacaaat cccaacagcg atgaagagat ttatctcagc
     3421 ccagcagtgg aacggatctt tggtcgcagt atgaatgacc ttatggcgag aagttcctcg
     3481 tttatagaaa gcattctgcc agaagatgtg ccgctggttc taacgaatat ggaaagacaa
     3541 aaactgggta ccgccaccaa tatggaatac cgcatcaaac gtccagatgg tcgcatccgc
     3601 tgggtctggg atcgtgcctt cccgatccta aacccgaatg gaacggtgcg gcttgtgacg
     3661 ggtattacca ccgacatcac agaacgcaag gcaaccgacg ccaaaattca aacgcagatc
     3721 aagcgtctca gcgccctcaa ctccatagac cgggcaatca gctccagcat gcgcatggat
     3781 gtagctctgg atgttctcct tattgaagtt gtcgcccagc ttaatgtgga tgctgcttcc
     3841 atccttcttt tgaacgaaac cgatcaaacc ttagacttcg cagcaggcaa agggtttaat
     3901 acttccatcg aacatgcaag tgtgccgctc gggtttgaac tggctggtaa agttggcact
     3961 gaacgaaaag taattcatgt acccaatctg tctgaattaa aaaaaggggt caaaaggaaa
     4021 gagcttatcc aagaagagca tttcctggaa tactttggtg tgcccttgat cgtaaaagga
     4081 caactcaaag gcgtgcttga gatattcaac cgcgaagcct tgaacccaga ccctgaatgg
     4141 ttaacctatc ttgaaacact aggcgggcaa gctgccattg ctattgaaag cgcacaacta
     4201 tttgaagggt tgcaacgctc caaccaggaa cttttcatgg catacgatgc caccatcgca
     4261 ggttggtctc atgccatgga tttgcgcgat aaagaaacag aagggcacac gcaacgcgtt
     4321 acagaaatca cagtccgcct agcagaacgc atgggaatca gtcaacagga attggtccat
     4381 ataagacgcg gagccctcct ccatgacatt ggcaagctgg gtgtcccaga tcatattctg
     4441 ctcaagccag ataaactcac cgatgaagaa tggcacatca tgcgcatgca ccccgtttac
     4501 gcgcgcgata tgctcactcc tatccactat ctgcgaccgt cgcttgatat cccttattgc
     4561 catcacgaaa aatgggatgg cacaggctat ccacgcggat taaaaggtga ggatatcccg
     4621 ctggcagcca ggctctttgc gattatagat gtgtgggatg cgctccgctc ggatcgtccc
     4681 taccgcgcgg gctggtctgc cgaaaagact cgcgactaca tccagtctga atctggcaaa
     4741 cattttgacc caaaagtcgt tgaatacttc ctggcacttc tggaagaact ttcaaaaagc
     4801 tgacaattca acaaaaaaga caacccttgt ggtatcacaa gggttgtctt tttctattag
     4861 gagtagatgt tacccatttt tgagatttat aacattgcag catcagcaaa gagagcaatc
     4921 atattgtaat ttcatctaac atagtgggta tgctatactg attttaagcc ccagagactt
     4981 gtggagtaga ggaaataatg gaatgaccgc catctctaaa gcaatgccca agaataaaaa
     5041 aggggaaact gggcagaaga gacaaataaa aacgagcaga gcaaattcac catctgctca
     5101 tcatcacatc atgaacgaag taaacctcga tattaacaac aaatccaaaa cagcaattca
     5161 gcgcctaaaa gctgagaatg cccgtctgca agcagacctt gaaacgttaa tgcgtgaacg
     5221 ccgcacgatg gcagaaactt ctttgagaca ttctcaagaa ttggatttat tggaaaaagt
     5281 tcgcgccacc atcgctacaa aattggaact gccagcactt ttacacagtg tcgtcgaatc
     5341 gattgtagaa atatgtggct acgcttatgt cagcatttat ctattagaag gtgaaacttt
     5401 aatccttcag catcaagcag ggtatcaaaa ggtcatcgaa aagatcaaca tccacgaagg
     5461 cattagcgga cgtgtcgccc gaactggtca acccattctt atagaagatg caaaaggcga
     5521 gccagaattt cttcaagcct caaaaaaaat acgttctgaa atcactgtgc cattaatgga
     5581 caacggtcaa gtgaccggca cgttgagcat agaaagtacg tccaaactca ccgatgccga
     5641 tctacggatc atgatggcat taggcgctga aatcaatgta gcgattgagc gttcacggtt
     5701 atatgaagac ctgcaaaaac gcaaccgcac tttatctgcc ttacaagaag caacgcttac
     5761 tatcataggc aagttagggc tgcccgatgc ggttcaaacc attttggctc aaaccgctca
     5821 attgttgaac acctcacatg gctatgttta tctggttcag ccgaatggca cacttcagat
     5881 ggcaaccggg ctgggcgtgt acccaaaata c
//
LOCUS       DAOREF010000049         5903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7248_length_5903_cov_4.106532, whole genome shotgun sequence.
ACCESSION   DAOREF010000049 DAOREF010000000
VERSION     DAOREF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7248_length_5903_cov_4.106532"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PKI33_01660"
     CDS             <1..1260
                     /locus_tag="PKI33_01660"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNM35733.1"
                     /translation="AVKAENLRPIEIFSIPRGDSEVTVEAFETTFNGMPVYFIGGEPI
                     HSSGSVYSLDAKLDAEKYAFFSLAAMELPKHINWQPDVIHANDWHTALSLYVNLTKRW
                     EAGAKHIANVLTLHNMPFMGADVRAILEQYRIQLAQTDLPEWARVLPLPLGLWASDAI
                     VAVSPGYAGEVLTEEFGCGLNEFMSLRKETLSGILNGIDTVSFDPETDKSISVNYTLE
                     TLDRRVENKELLQEKLGLKRDPNVPLLAIISRMDVQKGVDLAFTALKNMKRIDFQAVI
                     LGTGDPKLEEAARELQNLYPDKIKAELRFDAALARQIYAGADMILMPSRYEPCGLAQM
                     IAMRYGCIPVVRSVGGLKDTVIPNETGFIFEKAHHMSLVSAIKAALKIYPDREKWAAL
                     QRNGMAKDFSWEASAREYLKLYQSLVK"
     gene            1263..2768
                     /gene="malQ"
                     /locus_tag="PKI33_01665"
     CDS             1263..2768
                     /gene="malQ"
                     /locus_tag="PKI33_01665"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNM35734.1"
                     /translation="MAFKRSSGILLHPTSLPGPYGIGDLGPQAFRFVDWLSSTGCKIW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISPDDLLAENLVTEEDLVELKELPASKVDFG
                     LFIPKKLELLAKAFQRYQADPEPLREAFNYFCAENAYWLDDFALFMALKEANGGGAWN
                     GWDPALRARKKTALNKAKKEQAENVERFSFYQFLFFRQWTRLRKYANEKGIQIMGDIP
                     IFVAYDSADVWANPDLFFLDEAGNPTVVAGVPPDLFSTTGQLWGNPLYNWKVHKKDGY
                     AWWLSRVRALLQTVDILRFDHFRGFAGYYEIPASHTTAEHGRWVPGPAKDFFRAVDED
                     LGNGVIAPGTGLPIVAEDLGVITPDVIELLNAFDLPGMKVLQFAFTGPENPFLPHNYV
                     PNCVAYTGTHDNNTAKGWYADAPEAEKDFARRYLAVDGHDFSWDLIRAVWRSVAVFAI
                     APMQDVLGLGGEARMNFPSRLGGNWEWRMSESDFREDLAAGLRDLNWMTLR"
     gene            complement(2832..3293)
                     /locus_tag="PKI33_01670"
     CDS             complement(2832..3293)
                     /locus_tag="PKI33_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM35735.1"
                     /translation="MLTIDFLEDHLDTIPTLVKWFRNQWTEYYANSSDEAMQQDFLED
                     ILRDRLPSRLVAFEGNELVGTIVLREGDAELSPEHHPELGGLYVAESHRGHGIGTELI
                     KAGMKLAQDQGYSEISATTVKAAGILEGLGWKFIKKIQYPDGEVSLYHCKI"
     gene            complement(3385..3774)
                     /locus_tag="PKI33_01675"
     CDS             complement(3385..3774)
                     /locus_tag="PKI33_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35736.1"
                     /translation="MKTNKAFFTLVTITSIICLCIGCFFAFSMPVYKTLDNPTAEEGQ
                     QKANELIIALEQYKNDTGSYPSELGSLVPDYLSAIPQPTWSAHYSYELQPSEDEFIIS
                     FDVGISFDGDYCEYNSRTKNWHCSDKI"
     gene            complement(3811..5091)
                     /locus_tag="PKI33_01680"
     CDS             complement(3811..5091)
                     /locus_tag="PKI33_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM35737.1"
                     /translation="MRIVIIAPGSRGDVQPYIALGKGLQNAGHYIRLVSHSNFETLVT
                     SYGLEFWSFGNDVKDAVENSEMRELTEKGNFLLLMAKMAKEAEREALRFAEGGLLASQ
                     GMDVVLAGIGGLFIGTAIAEKLHMPLVQAYVFPFTPTKDFSSVLTPKLPTVFNRLSHH
                     LARQLMWQGFRSADGIARKEALNIPSAPFLGPYDSRSTNNMPVLYGFSPSVISTPSDW
                     NGNTHITGYWFIDETDDWQPPVALLDFLKSGPPPLYIGFGSMSNRNPEQTADLVIQAL
                     AMTNQRAILLSGWSGLQKTNVPDSILMVDSIPHSWLFPRVSAVIHHGGASTVAAGLRA
                     GVPSIIIPFFGDQPFWGQRIADLGVGTKPIPRKKLTAEKLANAIREVVTNQEMRQRAE
                     KLGKQIQSENGIKSAVEIFNKLEQQLPNKASKGN"
     gene            complement(5278..5781)
                     /locus_tag="PKI33_01685"
     CDS             complement(5278..5781)
                     /locus_tag="PKI33_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35738.1"
                     /translation="MSGPFGVSMTIVSGQDFSGEIVVAAATLLSMNWLSIHLISTDDH
                     IRSELLTALNRFGNKPGLYYQTQPSQFVERLTTMKHEIFSKDFFWLAMDKEGEIVASK
                     ILEENGQTPTKKVYQSVHKLNLMEPTITPPPLTPDEKSYMEWCASLYESLTPWQNIIK
                     NIQSDLS"
BASE COUNT         1518 a         1327 c         1328 g         1730 t
ORIGIN      
        1 gcggtgaaag cggaaaattt gcgccccatc gaaatttttt ccattccgcg cggggattct
       61 gaagttactg tcgaagcgtt tgaaactaca tttaatggaa tgccggtcta ttttattggc
      121 ggtgaaccga tccattccag cggctcggtg tattccttag acgcaaaatt ggatgctgag
      181 aagtatgctt tcttctcact ggcggcgatg gaactcccca agcacatcaa ctggcaacca
      241 gatgttattc atgccaacga ctggcatacc gcactgagtt tatatgtcaa tttgaccaaa
      301 cgctgggaag ccggtgcaaa acatattgcc aatgtgctta ccctgcataa tatgcctttc
      361 atgggggcgg atgtccgtgc tattcttgaa cagtatcgca ttcaattggc tcaaaccgat
      421 ttgcccgaat gggcacgtgt tttgccccta cccttagggc tttgggcgtc ggatgcgatt
      481 gttgccgtct caccggggta tgcgggggaa gtgctcacag aagaattcgg ttgtggcttg
      541 aacgaattca tgtctttgcg caaagaaacc ttgagcggta ttttaaatgg cattgataca
      601 gtttcttttg acccagaaac cgataaatcc attagtgtca attacaccct cgaaacactc
      661 gaccgccgag ttgagaataa agaacttctg caagagaaac tagggctcaa gcgtgacccg
      721 aatgttccgt tgcttgctat tatttcacgt atggatgttc aaaaaggcgt agaccttgcc
      781 ttcactgcgc taaagaatat gaagcgcatc gacttccaag ctgtgattct tggaaccggc
      841 gacccgaagt tggaagaagc ggctcgagag ttgcaaaatc tttaccccga taagatcaaa
      901 gccgagttaa gattcgatgc ggcacttgcc cgtcaaatct acgccggcgc cgatatgatc
      961 ctcatgccgt ctcgttatga gccgtgtggt ctcgcacaaa tgatcgccat gcgctatggg
     1021 tgtattccgg tcgtgcgatc tgtgggcggg ctaaaagata cggtcatccc aaatgaaacg
     1081 ggcttcatct ttgaaaaggc acatcatatg tcattggtca gtgccatcaa agccgcattg
     1141 aaaatttatc cagaccgtga aaagtgggca gccttgcagc gcaatggcat ggcaaaagat
     1201 ttttcatggg aagcttcagc acgcgaatac ctaaaactct atcaatcgtt ggtgaaataa
     1261 acatggcatt caaacgttct tccggtattc ttcttcatcc taccagtttg cccggtccat
     1321 atggcatcgg cgaccttggt ccacaagcat ttcgttttgt cgattggctt tcatctacag
     1381 gttgcaagat ctggcaaatt ttgccgttgg gtcctacagg gtatggcgat tcgccgtatc
     1441 aatgtttttc tgcctttgca ggaaaccctt acctcattag cccagatgat ttgcttgccg
     1501 aaaatttggt gactgaagaa gaccttgttg aacttaaaga acttcccgcc tcaaaagtgg
     1561 actttggctt gttcatccct aaaaagcttg agcttttggc gaaagcgttt cagcgctacc
     1621 aagccgaccc cgaacctttg cgtgaagcct tcaactattt ctgcgccgaa aacgcctact
     1681 ggctcgacga ctttgccctc ttcatggcat tgaaagaagc taacggtggt ggcgcttgga
     1741 acggttggga ccccgccttg cgtgctcgta aaaagacggc tttgaataaa gcgaaaaaag
     1801 aacaagctga aaatgtagag cgtttttctt tctatcaatt ccttttcttt cgccagtgga
     1861 ctcgcctacg caagtacgca aatgaaaagg gaattcagat catgggcgac attcccatct
     1921 tcgtcgccta cgactcagcc gatgtatggg caaacccgga tcttttcttc cttgatgagg
     1981 cgggtaatcc cactgtggtg gcaggggttc cacctgatct tttctcaacg actggtcaat
     2041 tgtggggtaa cccgctttat aactggaagg ttcacaaaaa agatggttat gcttggtggc
     2101 tttccagagt ccgcgctttg ttgcaaacgg tagatattct gcgttttgat cacttccgcg
     2161 gttttgcagg gtattatgaa attccagctt cgcataccac tgccgaacat gggcgttggg
     2221 tgccgggtcc tgccaaagac tttttccgcg cagtggatga ggaccttggg aatggcgtga
     2281 ttgccccagg aacaggtttg ccaattgttg ccgaagattt gggcgtgatc actccagatg
     2341 tgatcgaatt gttgaacgct tttgatttgc ctggcatgaa agttttgcaa ttcgctttta
     2401 cgggtccaga gaatcccttc ttgccgcata actatgtccc gaattgtgtt gcttacacgg
     2461 gcactcacga taacaatacc gccaaaggtt ggtatgctga tgctcctgag gcagaaaaag
     2521 attttgcccg ccgctacctt gccgtggatg gacacgactt ctcgtgggat ttgatccgcg
     2581 ccgtgtggcg gtctgtggca gtctttgcca ttgcccccat gcaagatgtg cttggcttgg
     2641 gcggagaagc ccgcatgaac tttccaagcc gcctcggtgg taactgggag tggcgcatgt
     2701 ctgaaagcga cttccgtgaa gaccttgctg caggtctgcg agacctcaac tggatgacct
     2761 tgagataaaa agagaccgcc tcgtatgagg cggtttcttt ttattgaggt gagatgcttt
     2821 tttgaattat cttagatttt acaatgatac aatgaaactt ccccgtctgg atattgtatt
     2881 ttcttgatga acttccaacc taaaccctct aagatgcctg ctgctttaac cgttgttgca
     2941 gaaatttctg aataaccctg gtcttgagct aatttcatac ctgctttaat caattctgta
     3001 ccaatgccgt gaccgcgatg agactctgca acatacaaac ctcctaattc aggatgatgt
     3061 tcaggcgata attcagcatc gccttctcgc agtacaatcg taccaacaag ttcattgcct
     3121 tcaaatgcaa cgagtcgaga gggtaagcgg tctctcaaaa tatcttcgag aaaatcttgc
     3181 tgcatggctt cgtctgacga attggcataa tactctgtcc attggttacg aaaccacttt
     3241 accaatgtag gtatagtatc aagatgatct tcaaggaagt caattgtaag catatttttt
     3301 tctgctttct taaagcagat acttttcggt tttagggttt gagaaacgat cttgtatatg
     3361 ctctgatttt actggttaga actatcaaat tttatcgcta caatgccagt tttttgtccg
     3421 actattatat tcgcaataat ccccgtcgaa acttataccc acatcaaagg agataatgaa
     3481 ctcatcctca ctgggttgaa gttcataaga ataatgggcg ctccatgtag gctgtggtat
     3541 ggcagataaa taatctggaa caagtgaacc taattctgag ggataacttc ctgtatcatt
     3601 tttgtattgt tccaaggcta ttatcaactc gttagccttt tgttgccctt cttcggctgt
     3661 agggttatcg agtgttttat acactggcat cgaaaaggca aaaaaacatc caatacataa
     3721 acatataata gatgtaattg ttacaagagt gaaaaaagct ttatttgtct tcattaggtt
     3781 tgcaatatgt catcctgctg ggacgttatc ctaattccct ttggacgctt tgttcggcaa
     3841 ttgctgttca agtttattaa atatttctac agcagatttt attccattct cggattgaat
     3901 ttgttttcct aacttttcag cacgttgacg catttcttga ttagtcacta cttctcgaat
     3961 agcatttgcg agcttttcag ccgtaagttt ctttcgcgga atcggttttg tccctactcc
     4021 taaatcagca atccgttgac cccaaaatgg ttggtctcca aaaaagggaa taataatgga
     4081 tgggactcca gccctgagac ccgcagctac tgtgctcgcc ccgccatgat gaataacagc
     4141 ggacacacgc ggaaacagcc atgaatgcgg aatagagtcc accattaata tcgagtctgg
     4201 cacatttgtc ttttgtagac cgctccaacc tgaaagcaag attgctcgtt gatttgtcat
     4261 tgctaatgct tggataacta aatcggctgt ttgttcaggg ttgcgattgc tcatactgcc
     4321 aaacccaata tacagtggcg gaggacccga tttcaagaaa tcaagcagag caacaggcgg
     4381 ttgccaatca tctgtttcat ctataaacca atagccagta atgtgagtat tgccattcca
     4441 atctgatggt gtagaaatta ctgaaggact aaaaccataa agaacaggca tattatttgt
     4501 cgatcttgaa tcgtaaggtc ctagaaatgg agccgatggt atgttcaacg cttcttttct
     4561 tgctatgcca tctgctgacc gaaacccctg ccacattagt tgacgggcaa gatgatgtga
     4621 caaacgattg aatactgttg gcagctttgg tgtaagaaca ctggaaaaat cttttgtggg
     4681 tgtgaaagga aaaacgtagg cttgaacgag cggcatatgt agtttttctg caattgcagt
     4741 gccaatgaat aaccctccta tgcctgccaa cacaacatcc attccttgtg aggctagtaa
     4801 accaccttcg gcaaaacgga gtgcttcacg ctcggcttct tttgccattt ttgccattag
     4861 caaaagaaaa tttcccttct cggtcaattc tcgcatttca ctattttcaa cggcatcttt
     4921 tacatcattt ccaaaagacc aaaattcaag cccgtatgat gtaaccaatg tctcgaaatt
     4981 gctatggctt acaagacgaa tgtaatgtcc tgcattctgc aagccttttc ctaatgcaat
     5041 ataaggttga acatccccgc gacttcctgg tgcaatgatt acgatacgca tcgtgtatga
     5101 attatcctct tatcaagaat atttttgtta cggtaactac tgaagtgcat taagttatgg
     5161 cgaagctgtc caaccagaaa aaggctcagt cgtagcaaac tgcttgggct gtgagctcgc
     5221 gctttgttgg gcgctttgtg caattaggct actattttat ccgtttggct ttattcatca
     5281 cgacaaatcc gattgaatat ttttgatgat attttgccaa ggggtcaggc tttcataaag
     5341 cgaagcacac cattccatat aagatttctc gtcaggtgtt agtggtgggg gtgttatcgt
     5401 tggttccatt aaattcagtt tatggacgga ctgatacacc ttctttgtag gcgtttgtcc
     5461 attttcctca agtattttgc tggctacgat ttccccttct ttatccatgg caagccaaaa
     5521 gaaatctttt gaaaaaatct cgtgcttcat ggtcgttaat ctttcaacaa attgacttgg
     5581 ttgagtctga tagtaaagac ctggcttgtt tccgaaccta ttcagtgcag tgaggagttc
     5641 agatcttata tggtcatctg tgcttatcaa gtgaatagac agccaattca tagacaagag
     5701 tgtagctgct gcgacgacaa tttcacctga aaaatcttgt ccacttacga ttgtcatact
     5761 gacgccaaat ggtccactca taattctcct ttgtgttgtg tgttcttaat atggtaccgc
     5821 ctaacggcat gcgttacctg cgctggggaa gggacggcga agccgtccag ccagaaaaaa
     5881 gctaaggcgt agaaaactgc ttg
//
LOCUS       DAOREF010000050         5888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7269_length_5888_cov_5.723813, whole genome shotgun sequence.
ACCESSION   DAOREF010000050 DAOREF010000000
VERSION     DAOREF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7269_length_5888_cov_5.723813"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..865)
                     /locus_tag="PKI33_01690"
     CDS             complement(116..865)
                     /locus_tag="PKI33_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNM35739.1"
                     /translation="MPASTLNLKSALSDLSLKKRLVLVHASPLVNADSLFTSLLETTS
                     GFFTPTFTPKSLLPLEYHPVHSKADVLSLAQPFTSSTPTDEAFGAFPELVRKHAKGKR
                     SIHPVFSFAGINADAILHSQTQDNLYAPIATLADEDGVILLVGMDQRVNFSIHYGEKL
                     AGRMQFIRWALTEDGVVECPNFPGDSEGFNAISLTVKPFTRQAEVDGITLQAIPVNDL
                     LKSVVNKIHEDAYSLLCGRPDCERCEAVRWG"
     gene            complement(946..1719)
                     /locus_tag="PKI33_01695"
     CDS             complement(946..1719)
                     /locus_tag="PKI33_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNM35740.1"
                     /translation="MTYTTILTETRGRVGIVQLNRPQAMNAFNLAMLGEVFDALEVFD
                     KDENVGAIIVTGNEKAFAAGADIKEMADQTYVQMLANEHVKIWDRIRGIRKPVIAAVS
                     GWALGGGSEFVLSCDMVIASESAKFGQPEITIGIIPGAGGTQRLARLVGKHLAMEMVI
                     NNRTLTAQEAQQFGLANRVVPVEGYLDAAVAFAEEIASRAPIAVRMAKDAVNAAFETT
                     LTEGLAVEKRNFYPLFATEDQKEGMQAFIEKRKPNWKGK"
     gene            1861..2259
                     /locus_tag="PKI33_01700"
     CDS             1861..2259
                     /locus_tag="PKI33_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35741.1"
                     /translation="MQILFLIHSHMRWLILIVALVAIVKFAIGWLRGGAFKGMDRGLA
                     SGFSGLMDLQATLGLIFLIWTGLAGVGFPMNRIEHATTMIIAAVLAHLPARWKNASDS
                     IRFRNTLFCIVGSLVLVFMGVVRLRGGWTW"
     gene            2308..2901
                     /locus_tag="PKI33_01705"
     CDS             2308..2901
                     /locus_tag="PKI33_01705"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNM35742.1"
                     /translation="MKKLILIGPVLFAVVLVTLTFVKYDFLLSLGWHPINDPTFDWPS
                     GLALGRFGWIMTATFITSGLIMTLFARRLFLDLKPGTASKLGTTLMAFAGLFLATLAF
                     TTDPTIRDTPATWHGQLHDLSFVLLGLTLFPAMIVLGFAFRADERWKNLAVYTWGTLA
                     LAGPAFFIKGAAFYVFLFAILAWNEIAAIRLNSHAEN"
     gene            complement(2936..5617)
                     /locus_tag="PKI33_01710"
     CDS             complement(2936..5617)
                     /locus_tag="PKI33_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM35743.1"
                     /translation="MLSENSELAHQGSTEVQRARKTARSTFFTALKFMATRLLTVLLT
                     IGLGTFITIVAANGGGAIDNAEKSRINAVTLRSVDPFATSAEELTKLRADMEEKEGLN
                     LPFLPRHMYWTYKALTLNWGEVNDRVKFHPRYTTSNMNAEDSRVILMQYLPNTILLIG
                     AGFFFLFIAGIPFALHLSQNPGGRLDKLIGTLTPLSSIPGWVIGFILILVFAVGLKIL
                     PVGRMIDSIPPESSFEYVLTVIRHMVLPVTAILLSSAFTLIFSWRTYFLAYSHEDYVD
                     VGKAMGLPQKTFRRNYILRPVLPAIITSFSLSMVGFWQLITTVEFVFDWPGIGSLFVR
                     ALPHFLSERMYPGEIPIIIGTMVIFAYLLGFTVFMLDIAYLVTDPRIRVTSGGSSQRL
                     VAKRTKRKWTFPTFKRPKFSWTETKQNFSDTKAQWAENWKKFLALLSELRGYPSAIIG
                     FSIVGLLVLTSIYTVIALPYRQIGRDWFLLSVKPRSVVPKLALPSWVNFFRENDLPST
                     ILIDSRTDSVERTETVLNETTKTITLTYTFDYPYTEFPKDMVIYYYPTYQEKVPFASM
                     ILTTPDGRELELRGEMTSFEKNYEPGSVLNINKILKQNPNWKEWFSIDEHYPTPAFYL
                     LFADPNAKTAKAVPGTYILTINAVGFEKDWDLDAQLVIFGQVYGMAGTDYMRRDLMTP
                     LLWGMPIALIFGLLGSIVITVGSAIVGAAAAWYGGWVDKALQVVIEANMILPVLAVGI
                     LLYAFYQVNFWLILMIIILLNIFGSPTKTFRAAFLQVKEASYVEAARAYGATNWRIIW
                     HYLIPRITPVMVPQLIALIPSFAFLEATFAIFNVSEVMYPTWGLVIQNAMKYGAAYGS
                     QFWVLEPISLLLLTGVAFGMSSAAFDYILNPRLRTKG"
BASE COUNT         1469 a         1340 c         1535 g         1544 t
ORIGIN      
        1 gaaggatgta gcgaatagga ttatggaatg gttgaaggaa aaaggaatcg agtaatccga
       61 aaataaaaag agtggaaagg ttttagcttt ccactctttt tatttaattc ttttattatc
      121 cccaccgaac ggcttcacag cgctcacaat cggggcgacc gcataggagg gaataggcat
      181 cttcgtggat tttattgacc acagacttga gcaggtcatt gactggaatg gcttgaaggg
      241 taatgccatc cacctctgct tgacgggtga agggcttgac ggtgagactg atggcattga
      301 agccttctga atcgccggga aagtttgggc attcaactac gccatcttcg gtcagtgccc
      361 agcggatgaa ttgcatgcgc cccgcaagtt tctctccata atgaatgctg aagttgacgc
      421 gttggtccat gccgacgagc aggatgactc catcttcatc tgctaaagtg gcgatgggtg
      481 cgtaaagatt atcctgagtt tgagaatgaa ggattgcatc tgcattaatg cctgcaaaag
      541 aaaagacagg atgaatggag cgtttccctt tggcatgttt gcgaaccagt tcaggaaaag
      601 cgccaaaggc ttcgtctgta ggcgtggagg atgtgaaagg ttgtgctaat gaaagtacgt
      661 ccgctttgga gtgaacgggg tgatactcta acggcaacag agatttgggc gtgaaggttg
      721 gggtgaagaa tccactggtg gtttccagca gggaggtaaa caacgagtcc gcattgacga
      781 gtggggaggc gtgcaccagc acaaggcgtt ttttcaggct taggtcggag agagcagatt
      841 tgaggttaag agtggaggcg ggcataggtt agtcgaatag tcaaatggtt gcatgtcgag
      901 tagtccaaat cgatcaagcg actactcgac gaaaaaacta caaggctact tacctttcca
      961 attcggtttg cgtttttcaa tgaaggcttg catgccttcc ttttgatctt cggtcgcaaa
     1021 taatggatag aagttacgct tctcgacggc tagaccttcg gtaagcgtgg tttcaaatgc
     1081 ggcattgacg gcatctttcg ccatgcggac ggcgatgggg gcgcgcgaag caatttcttc
     1141 ggcaaaggca acagcggcat cgagataccc ttcaacaggc acaacacgat tcgcgagacc
     1201 gaattgctga gcttcttgtg cggtgagggt gcggttgttg atgaccattt ccatggcgag
     1261 atgtttgccc acgagacgag ccagtcgctg tgtgccgcct gcgccgggga taatgccaat
     1321 agtaatttca ggctgaccga actttgcaga ctcgctcgcg atgaccatat cgcaggaaag
     1381 cacgaattcg cttccgccgc cgagcgccca acccgaaact gctgcaatga ccggtttacg
     1441 aatgccgcga atgcgatccc aaatctttac atgttcattg gcaagcattt gcacataggt
     1501 ttgatctgcc atttctttga tatcggctcc ggcagcgaag gctttctcat tgccggtgac
     1561 gatgatcgcg ccaacatttt catctttatc aaaaacttcc aatgcatcaa agacttcacc
     1621 aagcatggca aggttgaacg cgttcatggc ttgcgggcgg ttcaactgca caatgcctac
     1681 gcgtccgcgg gtttcggtca gaatggtggt gtaggtcatg ggttcctcca aagttcagat
     1741 agtgagatta taaactcaac ctgcactggg tataattctc aaaccctatt gggcagcgta
     1801 agaacggcta tatccgcttc atcccaaata tctgaactaa atttatatac ggagactctc
     1861 atgcaaattc tattcctcat tcattcccac atgcgctggt tgatcttgat cgtggcgttg
     1921 gttgccatcg tcaaatttgc cattggctgg ttgcgtggcg gcgccttcaa aggcatggac
     1981 cgcggtctgg cttctggttt tagcgggctg atggacttac aagccacgct cggcttgatt
     2041 ttcttgatct ggaccggttt ggcaggcgtt ggcttcccca tgaatcgcat cgaacatgcc
     2101 accaccatga tcattgcggc agtgcttgct catttgcccg cccgttggaa gaacgcctca
     2161 gattcgatcc gcttccgcaa taccttgttc tgtattgtcg gttcgttggt gttggtcttc
     2221 atgggtgtcg tccgcttgcg cggcggttgg acttggtaat ctaaaaaagg gaacagcctc
     2281 gcatatgcga ggctgttttg tttaaacatg aaaaaattaa ttcttattgg tcccgttctc
     2341 tttgcagttg tgcttgttac gctgacattc gtcaaatacg attttctttt gagtttgggc
     2401 tggcatccca tcaacgaccc caccttcgac tggcccagtg ggctggcgct cggtcgcttc
     2461 gggtggatca tgaccgccac cttcatcacc agcggactca ttatgactct tttcgcccgt
     2521 cgcctctttc tggacttgaa gccgggcaca gcttccaaac ttggcaccac gctgatggcg
     2581 ttcgctggtt tatttctcgc caccttggct ttcaccaccg atccaaccat tcgtgatact
     2641 cccgccactt ggcatggaca gctgcatgac ctctcgtttg tgttgcttgg actaacgctt
     2701 ttccccgcga tgatcgtcct tggctttgcc ttccgcgccg atgagcgttg gaagaatctt
     2761 gccgtctata cgtgggggac gcttgccttg gcaggaccag cattctttat caaaggcgca
     2821 gctttctatg tttttctatt cgcaattttg gcatggaatg aaattgcggc aattcgtttg
     2881 aactcacatg cagaaaacta acagcgtaac gaaaagccag agaaactgaa tcaacttatc
     2941 cctttgttct gaggcgcggg ttgagaatat agtcaaatgc cgcgctagac attccaaatg
     3001 caacaccggt caataagagc aaagagattg gttccaagac ccagaattgt gagccgtagg
     3061 cggcgccgta tttcatcgca ttttgaatga ccaagcccca tgtggggtac attacttcag
     3121 aaacgttgaa gatggcgaag gtggcttcga gaaaagcgaa actaggaatg agagcaataa
     3181 gttgcggcac catcaccggt gtgatgcgcg ggatgaggta gtgccaaatg atgcgccaat
     3241 tggtcgctcc ataagcacgc gcggcttcta cgtacgaagc ttctttgact tgtaaaaacg
     3301 cagcgcggaa ggttttggtg gggctgccga aaatatttag caagatgatg atcattaaga
     3361 tcaaccaaaa gttgacctgg taaaaggcgt ataacaaaat gccaactgcc agaaccggca
     3421 gaatcatatt ggcttcgatc accacttgca gggctttatc tacccagccg ccataccaag
     3481 cggcggctgc gcctacaata gcagacccaa ccgtgatgac gatggaacct agcaacccaa
     3541 aaatgagggc aatgggcatg ccccacaaaa gcggagtcat cagatcacgg cgcatgtaat
     3601 cggtaccagc catgccatac acttgtccaa agatcaccaa ctgggcatcg aggtcccagt
     3661 ctttttcgaa gccaaccgca ttgatggtta ggatgtatgt gccgggtact gccttggcag
     3721 ttttggcatt ggggtctgca aataataaat agaaagcagg tgtggggtaa tgctcatcga
     3781 tcgagaacca ttctttccag tttgggtttt gcttgagaat cttattgata ttgagcacac
     3841 tgcccggttc gtaattcttt tcaaaggacg tcatctcgcc acgaagttcc agttcgcgcc
     3901 catcgggtgt ggtgaggatc atcgatgcaa agggaacttt ctcttgatag gtcgggtaat
     3961 aataaatgac catatctttt ggaaattctg tgtaaggata atcaaaggtg taggtaagtg
     4021 tgatggtttt ggtcgtttcg ttgagtacgg tttctgtgcg ttcgacagaa tcggtgcggc
     4081 tatctatgag aatagtggat ggcaggtcgt tttcgcgaaa gaagttgacc caacttggca
     4141 aagccagttt gggaaccaca cttctcggtt tgacgcttaa taagaaccaa tctctgccga
     4201 tctgacgata gggcaaggcg atgacagtgt agatggaggt caataccaga agaccaacga
     4261 tgctaaagcc aatgatcgca gagggatatc cgcgtagttc acttaagagc gcaaggaatt
     4321 tcttccaatt ctctgcccat tgcgcttttg tgtcggagaa attttgtttt gtttctgtcc
     4381 atgagaactt tggtcgtttg aaggttggga atgtccactt acgcttggtg cgtttggcaa
     4441 ccagcctttg tgagcttcca ccagatgtga cgcggatacg tggatctgtg actagatagg
     4501 caatatccaa cataaagacg gtgaagccca acaagtaggc aaaaatgacc atcgtgccaa
     4561 tgatgatggg aatttcaccg gggtacattc tttcacttaa aaagtgaggc aaggcgcgta
     4621 cgaatagtga gccgatgccg ggccaatcga agacgaattc cacagtggtg atcaactgcc
     4681 aaaatcccac catggaaagg gagaaactag tgataatggc gggcaatacc gggcgcagga
     4741 tgtaatttcg tcggaaagtc ttttgcggaa gacccatggc ttttcctaca tctacataat
     4801 cttcgtgaga gtaagcgagg aagtaggtac gccaggaaaa gatcaatgtg aatgcgctgg
     4861 aaagcaaaat ggctgtaacc ggtaagacca tgtgtctgat aacggtgagc acgtattcaa
     4921 aggatgattc aggtggaatg ctgtctatca tccgcccgac agggaggatc tttaaaccca
     4981 cagcgaagac tagaatcaaa atgaaaccaa tgacccagcc cggaatggag gaaagaggcg
     5041 tgagcgtccc tatgagtttg tctaaccgcc caccggggtt ttgagatagg tgtagcgcaa
     5101 atggaatccc tgcaataaaa agaaagaaga agccagcacc gatcaaaagg atggtgttag
     5161 gaaggtattg catcaggatc acgcggctat cttctgcatt catattggaa gttgtgtagc
     5221 gcggatgaaa cttcactctg tcatttactt ctccccagtt cagagtcagt gctttatacg
     5281 tccagtacat gtggcgcggc agaaagggga gatttaaccc ttctttttct tccatatctg
     5341 ctcgtagttt agtaagctct tcggcagatg tggcgaaagg gtccacactc cgcaaggtga
     5401 cggcattaat gcgactcttt tctgcgttgt ctatggcacc gccaccattg gcagccacaa
     5461 tggtaatgaa tgtgcccaac ccaatggtga gaaggaccgt taacaatctt gtcgccatga
     5521 acttcagggc ggtgaagaac gtactccgag ctgttttgcg tgctctttgt acctctgtac
     5581 ttccctgatg ggcaagctct gaattttcag aaagcattcg gtttccttaa tcttggtcta
     5641 ggcaagttct actttgagat tatacattta ctaggaaaat gctcccccca ccgtagtatg
     5701 gttttaaatt ttgattttat gtggcgtaca attccgacta tgcatcttga acaactgatt
     5761 actcattttg gttatcccgc cctgttgttg ggactcttgc tggaaggtga aacggtccta
     5821 gtccttgcgg ctttcatggc gcatcgcgga tatttagact tgccagtggt tattctgctc
     5881 ggtggctt
//
LOCUS       DAOREF010000051         5879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7294_length_5879_cov_4.469437, whole genome shotgun sequence.
ACCESSION   DAOREF010000051 DAOREF010000000
VERSION     DAOREF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7294_length_5879_cov_4.469437"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKI33_01715"
     CDS             <1..181
                     /locus_tag="PKI33_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35744.1"
                     /translation="GLTPGKQFHLFSCAPFKGPLRLQMKPHDHLIGYELAASLWVEVT
                     MKGEGNKVPTSLASK"
     gene            complement(226..2703)
                     /locus_tag="PKI33_01720"
     CDS             complement(226..2703)
                     /locus_tag="PKI33_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNM35745.1"
                     /translation="MRYGHFDDINREYVIDTPETPLPWINYLGSESYFGLISNTAGGY
                     SFYKDARLRRLTRYRYNNSPLDMGGRYIYLRDNNAADSARVWSPSWMPARTPLDFYEC
                     RHGMGYTIIRSKFNGIESQTRYFVPMGENLEIWELTLTNHRDSAADISIFSAIEFNLW
                     DAMDDSSNFQRNYSIGQVEVVDDIIYHKTEYRERRDHFAYFACSEKLSGFDTQRESFL
                     GSYRGWDNPKAVEAGQATNSIAHGWQPIGSHHVNVQLKAGEQKKIVFVLGYHENPRDA
                     KFDPPESQIINKRTVLPIISKYLKTAEVDTAFEGLCSHWTTLLNKFQVNTPDEHTDRM
                     VNIWNAYQTMITFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHLIPERARERILDLA
                     ATQLETGGAYHQYQPLTKRGNNDVGSGFNDDPLWLVLAVAAYVKETGDFSILNEPVQY
                     DNKPGSEEPLYGHLVRSIQYTLDRLGPNQLPLIGRADWNDCLNLNCFSDSPGQSFQTT
                     TNKDGKVAESVFIAGLFVLAAKEMAELTEQVKTLKVNKAFDLRPSTHYLDAASTMETT
                     IWKAGWDGEWFRRAYDDFGHVLGSKENDEGRIFIEPQGICVMAGLGVEDGRAQKALDS
                     VDVHLATPHGVVLQQPAFSKYYLHLGEISSYPPGYKENAGIFCHTNPWIMIAEAKVGR
                     GDKALDYYLRINPSAREGIGDLHRCEPYVYAQMIAGKDAATHGEAKNSWLTGTAAWNY
                     VAITQWILGIRPTYEGLQVSPVVPVGWKSFEAVREFRSVRYNIKVERSGAGNQVKLEV
                     DGKAIAGNVIPLAANETKEVRVRVILS"
     gene            complement(2713..4212)
                     /gene="xylB"
                     /locus_tag="PKI33_01725"
     CDS             complement(2713..4212)
                     /gene="xylB"
                     /locus_tag="PKI33_01725"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNM35746.1"
                     /translation="MNYFLGIDTSTTSSKALLIDSNGVVIAVAAASHTLQTPKPLWSE
                     QNPREWWDAVAASIKSVLEKAGVSGERVVAVGLTGQMHGLILLDENGSVLRPAILWND
                     QRTQSQCDEIHQVIGKEKFIQITGNVALTGFTAPKILWVKENEPSVYARAKHVLLPKD
                     YIRFCLTGEYAMDKADGAGTVLFDLKARDWSDEVLAALGIPREWMPRTFEGTAFTGKV
                     TEEAARLTGLKAGTPVAAGGGDQAAGAVGVGAVEPGIVGLTVGTSGVVFATTPSALIE
                     PEGRLHAFCHAVPGMWHFMGVMLSAAGSLQWYRDTLAPNVSFDELVKEAESAPAGSEG
                     LLFLPYLSGERTPYPDPLARGSFVGLTLRHTRAHMTRAVLEGVAFGLKDSFTLIQNAG
                     LGKITQVRASGGGTKGALWRQILASALEAELVTVNTSEGTAFGAALLAGVGAGTWSDV
                     ASACKSTIQLTGQTLPDDAQVNSYRRSYPLYRELYPALKPSFEKISQTN"
     gene            complement(4218..5102)
                     /locus_tag="PKI33_01730"
     CDS             complement(4218..5102)
                     /locus_tag="PKI33_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35747.1"
                     /translation="METVNFFGQECVVFGNKAIQLLITRSVGPRILSLKFTGGENLLA
                     EMPDFITDCPGSGIYHFYGGHRLWHAPEVPSRTYLPDDSPVDITQIENTLHIVQEVEA
                     KTGLQKSFDIQMQGDCASLSITHRLTNKGLWSATTALWAITQFKPGGLGILPQTVENT
                     GVLPNRRLTFWPYTDPSEPAVHLGKNYILINANMQGAFKVGFSNPRGWQAYWWNGVLF
                     VKRAKYEADAEYFDYGSSSESYCNHEILELETLSPITTIEPGAEATHVETWEMYQGIE
                     RPQNEQDVQALVERLELE"
     gene            complement(5123..>5879)
                     /locus_tag="PKI33_01735"
     CDS             complement(5123..>5879)
                     /locus_tag="PKI33_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNM35748.1"
                     /translation="SLLSPQNGDRESISLPQSQIDYIKELSIHGARIILVLTGGSPIA
                     LGEVEDLVEAILFVWYPGMEGGRAVADVLFGDVSPAGKLPITFPKSLDQLPAFDDYSM
                     TGRTYRYMNSEPLYPFGFGLSYTCFEYSNLKLDKTNLALGDSLTVNLTVRNSGGSDSA
                     EVVQYYLSDLHASTIVPLHHLIGFERVHLKAGESKTLTFTITPDMMSFYNDDGKLTLE
                     PGEFRVEVGSCSPGKRGQELGAPAPVTALFEVK"
BASE COUNT         1392 a         1491 c         1498 g         1498 t
ORIGIN      
        1 agggcttacc cccggtaagc aatttcactt gtttagttgt gcaccgttca aaggtccgct
       61 gcgattgcag atgaaaccgc atgaccatct cattggttat gaacttgctg catccctttg
      121 ggtggaagtg accatgaagg gcgaaggaaa taaagtgccc accagtctgg cgtctaaata
      181 aaaaacatcc cgatttcggg atgttttttt atcaagaaaa atccactaac tcaatatcac
      241 acgcacacgt acttctttgg tttcattcgc agccaagggg atgacatttc ctgcaatggc
      301 ttttccatcc acttccagct ttacctgatt cccagcaccg cttctttcga ccttgatgtt
      361 gtatcgcaca cttctgaatt ctctcactgc ctcgaacgat ttccacccaa ccggaacaac
      421 cggcgagact tgcaaccctt catacgtcgg gcggattcca agaatccact gcgtgatggc
      481 aacataattc caagcggcag tgccagtgag ccaggaattc tttgcctcgc cgtgagtcgc
      541 agcatctttg cccgcgatca tttgagcata gacatacggc tcacagcgat gcaaatcccc
      601 tatgccttcc cgcgccgatg ggttgatgcg taggtaatag tcaagcgctt tatctccgcg
      661 cccaactttt gcctctgcga tcatgatcca tggattggta tggcagaaaa tacccgcgtt
      721 ctccttgtag cccggcgggt aggaagagat ctcacccaag tgcaaataat atttgctgaa
      781 ggctggctgt tgtaatacca ctccgtgcgg tgtcgccaag tggacgtcca cggagtccaa
      841 agccttttga gcgcgaccat cttctacgcc caagcctgcc atcacacaaa tcccttgcgg
      901 ctcgatgaag atgcgccctt catcattttc tttggaccct agcacatgac caaaatcatc
      961 ataggcgcgg cggaaccatt cgccgtccca gccggctttc cagatggtgg tttccatggt
     1021 ggaggctgca tctagataat gtgttgacgg tcgaagatcg aaagccttgt tgacctttaa
     1081 cgttttgact tgttctgtta actccgccat ttcttttgct gctaacacga acaaaccggc
     1141 aataaaaaca ctctctgcca ctttgccgtc tttgttggtt gtggtttgga aggattgacc
     1201 aggtgaatca gagaagcagt tgaggttcag gcagtcgttc caatctgcac gcccgatcaa
     1261 cggcagttga ttgggtccga gcctgtcgag tgtgtattga atcgagcgga ctaaatgtcc
     1321 gtaaagcggc tcttcactgc cgggcttgtt atcatactga acgggttcgt tgagaatgct
     1381 gaagtcgccg gtttctttga catatgccgc aaccgccaac accaaccaca atgggtcgtc
     1441 gttgaagccc gagccgacat cattgttgcc gcgtttggtc agtggttgat attgatgata
     1501 ggcgccgccg gtttcgagtt gggtggctgc cagatccaaa atacgttcac gggcgcgttc
     1561 agggatgagg tgaacgaaac cgagcaaatc ttgatttgag tcacggaagc ccatgccacg
     1621 cccaatgccg ctttcaaagt atgaagccga gcggctcatg ttgaaggtga tcatggtttg
     1681 gtaagcattc cagatattga ccatgcggtc tgtatgttca tcgggagtgt tcacttgaaa
     1741 cttattgagc aaagtcgtcc aatgggagca tagaccttca aaggctgtat cgacttcagc
     1801 ggtttttaga tacttggaaa tgatcggcaa gacggtgcgt ttgttgatga tctgtgattc
     1861 aggcgggtcg aacttggcgt ctctggggtt ttcgtgatac ccaagcacaa agacgatctt
     1921 cttctgttcg cctgccttta attgcacgtt gacatggtgt gacccaatgg gctgccagcc
     1981 atgcgcaatt gaatttgtag cttgacctgc ttccacggct ttggggttat cccatccgcg
     2041 ataggagccg aggaaagatt cacgttgagt atcaaagccg ctcagttttt cggagcaggc
     2101 aaaataggca aagtggtcgc ggcgttcacg atattcggtc ttgtgataaa tgatatcgtc
     2161 caccacttct acttgaccaa tggagtaatt gcgctggaag ttgctcgagt catccatggc
     2221 gtcccataga ttgaattcga tggcagagaa gatggagata tcagcagcgg aatcgcgatg
     2281 gttggtgaga gtcagctccc aaatttcgag gttctcgccc atgggcacga agtatcttgt
     2341 ttgagattcg atgccgttga acttggaacg gatgatggta tagcccatgc catgacggca
     2401 ttcgtagaaa tctaacggcg ttcgcgcggg catccagctt ggcgaccaga cccgagcgga
     2461 gtctgcggcg ttgttatccc tcaagtagat gtaacgcccg cccatatcca atggggaatt
     2521 gttgtagcga tagcgagtca agcggcgcag gcgtgcatct ttataaaagg aatatccgcc
     2581 agcggtgttg gagatgagac cgaaatagga ttcactgcca aggtagttga tccagggcag
     2641 tggggtctca ggagtatcaa ttacatattc gcgatttatg tcatcaaaat gtccgtaacg
     2701 catataacct ctttaatttg tttggcttat tttttcaaag ctgggtttca acgccgggta
     2761 tagctcacga taaagcggat acgaccggcg atagctgttt acttgggcgt catcgggcag
     2821 ggtttgcccg gtaagttgaa tcgtagattt acatgccgat gcgacatcgc tccatgtgcc
     2881 tgcgccaaca cctgctagta gagcagcacc aaaggctgtc ccttcggatg tgttgacggt
     2941 gaccaattct gcttcaagtg ccgaggcgag aatctgacgc cacaatgcgc cctttgtccc
     3001 gccgcccgaa gcgcggactt gtgtgatctt gcctaagcct gcattttgga tcaaggtgaa
     3061 gctatctttc aaaccaaatg ccacgccttc taacacggca cgagtcatat gtgcgcgcgt
     3121 gtgacgaagc gttagcccaa cgaaggaacc gcgtgcgagt gggtctgggt aaggtgtgcg
     3181 ttcaccgctg agataaggaa ggaacaacaa accttccgaa ccagcgggcg cactctcggc
     3241 ttctttcacc aattcatcaa agcttacatt gggcgcgagg gtgtcgcgat accactgcaa
     3301 acttcccgcc gcagaaagca tcacacccat aaaatgccac atacccggca cagcatgaca
     3361 gaacgcatgc aagcgacctt caggttcgat caatgcggaa ggcgttgttg caaagaccac
     3421 accgctggtg ccaaccgtca gaccaacaat gcccggttct accgccccca cacccacagc
     3481 gccggctgcc tggtcaccac cgcctgcggc gacaggtgtg cctgctttca aaccagtgag
     3541 acgcgctgct tcttcagtca ctttgcctgt gaaggctgtg ccttcaaacg tgcgcggcat
     3601 ccattcgcgc ggaatgccca atgccgccaa aacttcatcg gaccagtcgc gtgctttcaa
     3661 atcaaacaaa acggtgccag cgccatcggc tttgtccatg gcgtattcgc ctgtcaaaca
     3721 aaagcgaatg taatctttgg gcaacaacac atgttttgct cttgcataca cactgggctc
     3781 gttctctttc acccataaaa ttttcggcgc tgtaaaaccg gtcaacgcta cattgccggt
     3841 gatttgaata aatttttcct tgccgatcac ttgatgaatt tcatcgcact gactttgcgt
     3901 gcgttggtca ttccacaaga tggcaggacg caacacactc ccgttttcat ccaaaaggat
     3961 taagccatgc atttgacccg tcagcccgac agcgacgact ctttctccgc tcacgcctgc
     4021 cttttccaac acagacttga tactcgctgc aactgcatcc caccactcgc gcgggttttg
     4081 ctccgaccat aacggcttgg gtgtttgcaa cgtatgcgat gccgcagcga ccgcaatcac
     4141 cacgccattt gagtcgatca ataatgcctt gctagatgtg gtggacgtgt caattccgag
     4201 aaaatagttc ataagcttta ctccaattcc aggcgttcta ccaaggcttg aacatcctgt
     4261 tcattttgtg ggcgttcaat accttggtac atttcccatg tctctacatg agtagcttct
     4321 gcgccgggtt ctatggttgt gatgggagat aaggtctcaa gctcaaggat ctcatggttg
     4381 caataacttt cgcttgaact accatagtca aaatactcgg catctgcctc atatttggcg
     4441 cgttttacga acaaaactcc attccaccaa taggcttgcc aaccgcgcgg gttcgagaag
     4501 ccaactttga acgctccctg catgttggcg ttgatcaata tatagttctt tccgaggtgc
     4561 acagccggtt cagatggatc ggtatagggc cagaaggtga gcctgcggtt gggcaataca
     4621 cctgtatttt caaccgtttg cggcaagatg ccaagtccgc ccggcttgaa ttgagtgatt
     4681 gcccataaag cggtggttgc cgaccaaagt cctttgttgg ttaaacgatg tgtgatgctg
     4741 agagatgcgc aatccccctg catttgaata tcgaatgatt tttgtaaacc ggtttttgcc
     4801 tctacttctt gaacgatgtg taatgtgttt tcgatctgtg tgatatctac tggcgagtca
     4861 tcgggtaagt aggtgcggct tggcacttcg ggggcatgcc ataggcgatg ccccccataa
     4921 aagtgataaa tgccagaacc ggggcaatct gtaatgaagt ctggcatttc tgcgagcagg
     4981 ttttccccac ccgtgaattt caaagagaga atgcgcggac ccactgaacg ggtaatcaat
     5041 agttgtatgg ctttgtttcc aaatacaaca cattcttgcc caaagaaatt cacggtttcc
     5101 atctatgtca actccaatgt ttttatttga cctcgaacaa cgccgtgacc ggtgctggag
     5161 cgcctagttc ttgtccgcgt ttgccggggg agcagcttcc cacttccacg cggaactctc
     5221 caggttcgag agtgagtttg ccatcatcat tatagaagga catcatgtct ggtgtgatgg
     5281 tgaaggtcaa cgttttgctt tcgcctgctt tgagatgcac acgctcaaaa ccaatgagat
     5341 gatggagggg aacaatggtc gaggcgtgga ggtcgctcag gtagtattga accacttcgg
     5401 ctgagtctga gccgccgctg ttgcggacgg tcaggttgac ggtgagagag tcgccaaggg
     5461 ctagattcgt tttatcgagt tttagattcg aatattcaaa acaagtgtag ctcaagccaa
     5521 acccaaatgg atagagcggc tcgctattca tgtagcgata ggtgcgcccg gtcatgctgt
     5581 aatcgtcgaa ggctggcagt tgatcgagcg acttgggaaa ggtgatgggc aatttgccag
     5641 caggcgagac atcgccaaag agcacatcgg caacagcgcg accgccttcc atgccgggat
     5701 accaaacgaa gagaatggct tccaccaggt cttccacttc gcctaaggca attgggctgc
     5761 cgccagtgag cacgaggatg atgcgagcgc catgaataga aagttctttg atgtaatcaa
     5821 tttgactttg cgggagcgag atgctttcac ggtcgccatt ttgcggagag agcagtgac
//
LOCUS       DAOREF010000052         5868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7315_length_5868_cov_5.568897, whole genome shotgun sequence.
ACCESSION   DAOREF010000052 DAOREF010000000
VERSION     DAOREF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_5868_cov_5.568897"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKI33_01740"
     CDS             complement(<1..1108)
                     /locus_tag="PKI33_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNM35749.1"
                     /translation="MNLDFINKLNPQQRSAVTAADGPVMVVAGPGSGKTRVLTQRIAY
                     LIAHEGVRPWQILAVTFTNKAAKEMDERVKSILNEQATEGMMLGTFHSICARILRREA
                     EHLPVAQNFVIFDADDQQSLVKSIIREMNLNEKLYRANSMHASISRAKNELILPDDYP
                     INTYRDEVVKRVYTEYQKRLIASNAVDFDDILVYATQLMESNPAVREKYAQRFRHVLV
                     DEFQDTNLAQYALVKNLASYHKNIFCVGDPDQCFPAGVKIQTPHGQKKIEDIKRGDEV
                     TAASGRGSAMTSKVTHAGKRAFKGNLVRAKTKTGHIIRATPNHIMFARLGMKPGLHYV
                     YLMYRKDKGYRVGIASHARSDGSSLKVGLQVRSNQ"
     gene            complement(1185..1574)
                     /gene="mscL"
                     /locus_tag="PKI33_01745"
     CDS             complement(1185..1574)
                     /gene="mscL"
                     /locus_tag="PKI33_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNM35750.1"
                     /translation="MIMLKEFKEFVMRGNVLDLAVGVIIGGAFGKIVGSLVNDVIMPA
                     IGLLMGGVNFSELTIAGVIKYGAFIQTIIDFLIVAFVIFLIVKSANKMKKAPAPADPT
                     TKECPHCISTISIKATRCPNCTSELKA"
     gene            2004..4541
                     /locus_tag="PKI33_01750"
     CDS             2004..4541
                     /locus_tag="PKI33_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM35751.1"
                     /translation="MLRNRMFALLGLLVIASMVLAACGAPAATTEEAAPATEAAAPAT
                     EAATAEPTPVPTTRHGGWLDEIAYSSVTADSAVTQITAGAIDIYTRGLASDRVQEIKD
                     AGVCYAQSYGGYYDILFTPAVFTDTAKLNPFSNQKLREAMNWAIDRNYINQEIYGGGS
                     LAKYTVFTTQLVDYTGVIETARGLESYYAYNFDQAKAVVDAEMPAMGAELVDGKWAFN
                     GEPIVLAFLIRNDGDGTRLKIGDYFSDQLEKLGFTVDRQYRKSSEASPIWIGSDPKEG
                     QWNLYTAGWVSSGLTRDEKTGFQDMYLPSSSQGIPLFLENASDPAFVEAGDALANGAF
                     STLEERRALIEKALPLSLKDSLQVWIVDQQVYSPYQCNVEATYDLGSGLEAAQMPPYN
                     IRFKDQEGGQLKVGTNDLFTDPWNPVGGSNWVWDGFVQKATWSGSLMADPYTGLYWPL
                     RAESADVTVQTGLPVITNTDWVTLNTADTIEVPADAWSDWDATAQTFVPAGEGVTAKA
                     KIVMTYPADFFEVTKWHDGSNISVGDFVMRMIMTFDQAKPESAIYDEDSVPSYEAYKS
                     TFKAVKIVSTDPLVIETYTDRFYSDAELIATAGDWWPQYGFGEASWHAIAMANAAEAA
                     GETAYGTGKADRNAVEWTNFIGGPSLDLLNGQLDTLAAASTVPYAATLGQYVTADEAA
                     ARYANLKAFFEANGHFWSQTGPYYLASVDLNAKTALVKNNADFPDLSDRWASWSNPPL
                     ADATLDGPAQVKIGEATDFALTVTNKAGDAYPSADIKQVKFLVYNDKGETIYVGEGVA
                     GAGDGEYTLTIPADVQLQAGAGKIEAAVVLYPAAIPAFSALEFVAAP"
     gene            4809..>5868
                     /locus_tag="PKI33_01755"
     CDS             4809..>5868
                     /locus_tag="PKI33_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35752.1"
                     /translation="MATETITATTGQVKKESLANTTIGRVLRYALVRLVTLAITVVIG
                     IYLTILIANMGGYVDTIMRNEIRDNTTQSILANPAFRQLTPEAREKLIQEKIANEEKR
                     LGLDKPFINRSFTYLVNGLTLNLGRAVNMNSDSGSRQVRLILLERLPATLLLAGISDL
                     ALFFGSVFLALGLSRKYGSFFDKLTVALSPTSAAPPWFYGIFLILIFAGVLHVLPFGG
                     LVDSPPPATSFGYAMSVLKHIILPASSIIVSSFFISVYNWRTFFLIYSSEDYVEMARA
                     KGLPSRDIERRYILRPTLPTIITNFALLIIGMWTGFTITETVFLWPGLGRTLFQAIGL
                     YDTPVIVGSTITYAYLLGI"
BASE COUNT         1339 a         1440 c         1433 g         1656 t
ORIGIN      
        1 cctgattgct gcgaacttgt aagccaactt ttaatgaaga accatcgctg cgggcgtgag
       61 atgcaatgcc aacgcgatag cccttatctt ttcgatacat caggtagaca taatgcaagc
      121 cgggtttcat accgagccta gcaaacatga tgtggttggg cgttgctcgg atgatgtgtc
      181 cggtttttgt ttttgcacgg actagattgc ctttgaatgc ccgcttcccg gcgtgtgtta
      241 ctttggaggt cattgctgac ccacgtccac tggcggcagt gacttcatcc cctcgtttga
      301 tgtcttcgat tttcttttgc ccgtgaggag tttgaatttt tacgcccgct ggaaaacact
      361 gatcgggatc gcccacgcag aaaatattct tatgatagga agcaagattc ttcaccaaag
      421 catactgcgc gaggttggta tcttggaatt catctactaa aacatgccga aaacgctgtg
      481 catatttttc gcgtacggcg gggttgcttt ccatcaactg tgtggcgtac acgaggatgt
      541 cgtcaaagtc cacggcgttg ctggcgatga gccgtttttg atattcggta tacacgcgct
      601 tcaccacttc gtcgcgatag gtgttgattg ggtaatcatc cggcaagatc aactcgttct
      661 tggcgcgcga gatcgaagca tgcatactgt tggcgcgata caacttctca ttcaagttca
      721 tctcacggat gatggacttg accaaactct gctgatcgtc ggcgtcgaag atgacaaagt
      781 tctgcgccac gggcaggtgc tcggcttcgc ggcgcagaat ccgcgcacag atggaatgga
      841 atgttccaag catcatgcct tcggtcgcct gttcgttcaa aatggatttg acgcgctcgt
      901 ccatttcttt ggcggctttg ttggtgaagg tgacggcaag gatctgccac gggcggacgc
      961 cttcatgggc gataaggtat gcgatgcgtt gagtcagtac acgcgtctta ccggacccgg
     1021 gacctgccac gaccattaca ggtccatccg ccgctgtgac cgcgctgcgc tgctgtgggt
     1081 tgagtttgtt gatgaaatct aaattcatgg tgcggacatt ataaatgctc cgacaagtac
     1141 aaacccttgt cggagcattc agtctgacgt ttattaagga aagactacgc ctttaactca
     1201 gatgtacagt tcgggcaacg ggtggctttg atggaaatgg tgctgatgca atgcgggcat
     1261 tccttggtgg ttgggtctgc tggggcaggg gctttcttca tcttgttggc agactttact
     1321 ataaggaaga taacgaaggc tacaatgagg aagtcaatga tggtttggat gaaagcgccg
     1381 tatttgatga caccggcaat tgtcagttcg ctgaaattga caccccccat aagcaaacca
     1441 atggcaggca tgataacgtc attaacgaga gagccaacga tcttgccgaa tgcgccgccg
     1501 atgatgacac cgactgcaag gtcgagtaca tttccgcgca tgacaaattc tttgaattcc
     1561 tttaacatga tgatatctcc ttgatgaaat gataaatgaa ttgtatggca ggggaggggg
     1621 attgtcaacg aaaggcatcg gttgggtaga aatgctaaat gggttgaaac aggtttcggg
     1681 tctggtttga ggcggggctt tttgtcgaat tgtcatacaa agttttatta atattttctg
     1741 aaaacagtcc tctagtaggg tcaatttttc ctttctgcat attagactcg ggctgaaatg
     1801 tgactataca ttcctcttcc gcagcacccc tgttgactag cctaaagaat taggtataat
     1861 tccttgtgat tcttttttaa gtttgtaaca gcctcttaaa aaattaagaa ggaggtgatg
     1921 cgctaagatc gtaaacgtaa cgtgtgtttg tattgtgcct gtacgttaaa cccctaattt
     1981 tttcttcttg gaggagaaaa caaatgttac gaaaccgaat gtttgcttta cttggtttgt
     2041 tggtaatcgc tagcatggtc cttgcggcct gtggcgctcc tgccgcaacc actgaagaag
     2101 cagcccctgc cactgaagca gcagctcctg ctactgaagc ggcaaccgca gaacccactc
     2161 ctgttcctac cactcgtcat ggtggttggt tggatgaaat cgcctactct tctgtaaccg
     2221 ctgactctgc tgttactcag atcaccgctg gtgctattga tatttacacc cgtggtttgg
     2281 cttctgaccg cgttcaggaa atcaaggacg ctggcgtctg ctacgctcag tcctatggtg
     2341 gttattacga catcctcttc accccggctg tcttcactga taccgccaaa ctgaacccct
     2401 tcagcaacca gaagctccgc gaagcgatga actgggctat tgaccgcaac tacatcaacc
     2461 aggaaattta tggtggtggt tctttggcca agtacaccgt gttcaccact cagttggttg
     2521 actacaccgg tgtgatcgaa accgcccgcg gtctcgaatc ctactatgct tataactttg
     2581 atcaggccaa ggctgttgtt gatgctgaaa tgcctgcgat gggtgctgag ctcgtagatg
     2641 gcaagtgggc tttcaatggt gaacccattg ttctcgcctt cctcatccgc aacgatggtg
     2701 atggtacccg cttgaagatc ggtgattact tctctgacca gctcgaaaag cttggcttca
     2761 ctgttgatcg ccaataccgc aaatcctctg aagcttcccc aatctggatc ggttctgatc
     2821 ctaaggaagg tcagtggaat ctttacaccg ctggttgggt ttcctctggt ctcacccgcg
     2881 atgaaaagac tggcttccag gatatgtacc tcccctccag cagccagggt attcctctgt
     2941 tcctcgaaaa tgcttctgac cccgcttttg ttgaagctgg tgatgctctc gccaatggcg
     3001 ccttctccac tcttgaagaa cgccgcgctt tgatcgaaaa agccctcccg ctttccttga
     3061 aagactcctt gcaggtttgg atcgtcgatc agcaggttta ctcaccttac cagtgtaatg
     3121 ttgaggcgac ctatgacctc ggctccggtc tcgaagctgc tcagatgcct ccttacaaca
     3181 tccgcttcaa ggatcaagaa ggtggtcaac tcaaggttgg tacaaacgac ctgttcaccg
     3241 atccttggaa ccctgtcggt ggttctaact gggtttggga tggtttcgtt cagaaggcca
     3301 cctggagtgg tagcttgatg gctgatccct atactggttt gtactggcct ctccgcgctg
     3361 aaagcgctga tgtgaccgtt cagactggtt tgcccgttat caccaatacg gactgggtca
     3421 ccctcaacac tgctgacacc attgaagttc ctgctgatgc ttggtccgac tgggatgcca
     3481 ctgcgcagac cttcgttcct gctggtgaag gcgtaaccgc caaagccaag atcgtcatga
     3541 cctaccctgc tgacttcttc gaagttacca agtggcatga tggcagcaac atttctgttg
     3601 gcgactttgt gatgcgcatg atcatgacct tcgatcaggc caagcccgaa agcgccattt
     3661 acgacgaaga ctctgttcct agctacgaag cttacaagag caccttcaag gctgtgaaga
     3721 tcgtttcgac cgacccgctc gtgatcgaaa cctacaccga tcgcttctat tctgacgctg
     3781 aattgatcgc cactgctggc gattggtggc ctcagtacgg cttcggtgaa gcttcctggc
     3841 acgccattgc tatggcgaac gctgctgaag ctgctggtga aaccgcttat ggcaccggta
     3901 aggctgaccg caacgctgtt gaatggacca acttcattgg tggtccgagc ctcgacctct
     3961 tgaatggtca gctcgacacc ttggctgctg cctccaccgt tccgtatgct gctaccctcg
     4021 gtcagtacgt aactgctgac gaagctgctg ctcgctacgc caacctgaag gctttcttcg
     4081 aagccaatgg tcacttctgg agccagactg gtccttacta cctcgcttcg gttgacctca
     4141 acgccaagac tgctttggtc aagaacaacg ccgacttccc agatctctct gaccgttggg
     4201 cttcttggag caatcctcct ttggctgacg ccacattgga tggtcctgct caggttaaaa
     4261 ttggcgaagc cactgacttt gctttgaccg tgaccaacaa ggctggtgat gcctatcctt
     4321 ctgccgatat caagcaggtt aagttcctgg tctacaatga caagggcgaa accatttacg
     4381 tcggtgaagg cgttgccgga gctggcgatg gtgaatacac cctcaccatt cctgccgatg
     4441 ttcaactcca ggctggtgca ggcaagattg aagctgccgt tgtgctttac ccggctgcca
     4501 ttcctgcctt ctccgcactt gagttcgttg cggctccgta agtttttttc ttgaaattgc
     4561 caggggcaag ggaaaccttg cccctggttt ttcctgtaaa cggtgtattc ttagtagtgg
     4621 cggggaaaaa ctttaacaaa gtttccttag caaaaagata aactccgttt atattgaata
     4681 gttattttga aaatatgttt tgatggaatt atttttgaat tattaaaaga attacatgac
     4741 cagatacaag acttcatttt ttattgcttg taaaaaggtc acaattaaag aatgaattga
     4801 ggtgcgatat ggctaccgag acaattacag ccacgaccgg acaggttaaa aaagaatcac
     4861 tcgcaaatac tacgatcgga cgagtattgc gttatgcctt ggtcaggttg gtgactcttg
     4921 cgattactgt ggtaattggt atttacctta cgatcttaat cgctaatatg ggcggctatg
     4981 tagatacgat tatgcggaat gaaatccgtg ataatacaac acaatctatt cttgccaacc
     5041 cagcatttcg ccagctgact ccggaagcac gcgagaagtt gattcaagaa aagattgcca
     5101 atgaagaaaa acgtctgggt ttggataagc cctttattaa tcgatctttc acctatttgg
     5161 tgaatgggct gacccttaac ctaggacgtg cagtgaacat gaatagcgat agcggttcgc
     5221 gtcaggtccg tttgatctta ttagaacgcc tgcctgctac tctgttgctt gcaggtatct
     5281 ctgacttggc actgttcttc ggtagcgtat tcctagcgtt agggctgtct cgtaaatacg
     5341 gcagtttctt tgataaattg accgtggctt tatcgccgac ctctgccgct ccaccttggt
     5401 tttatggcat ctttctgatc ttgatctttg caggtgtact ccatgtcctc ccattcggtg
     5461 ggttagtgga ctcacctccg cctgccacct cttttgggta tgcaatgagt gtccttaagc
     5521 acatcattct gccggcttca tcgatcattg tcagttcctt cttcataagt gtttataact
     5581 ggcgtacatt cttcttaatc tattccagtg aagactacgt agaaatggca cgagcaaaag
     5641 gtcttccttc tcgtgacatt gaacgccgtt atatccttcg cccaactttg cctacaatca
     5701 ttacaaactt tgcgttgctc atcatcggca tgtggacagg ttttaccatt acggaaactg
     5761 ttttcctgtg gccaggcttg ggacgaactc ttttccaggc aatcggcttg tatgatacgc
     5821 ctgtgattgt gggttcaaca atcacttatg cttatttgtt aggcatta
//
LOCUS       DAOREF010000053         5844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7360_length_5844_cov_4.366730, whole genome shotgun sequence.
ACCESSION   DAOREF010000053 DAOREF010000000
VERSION     DAOREF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7360_length_5844_cov_4.366730"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..308)
                     /locus_tag="PKI33_01760"
     CDS             complement(60..308)
                     /locus_tag="PKI33_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35753.1"
                     /translation="MQKPKTKFDQKWKLIRGHSMEWWSLIAEHDLKKVDKADDKLDKF
                     LTILQVKYGYTRVEARDEVNRRWTAYSRAKTIVESLAS"
     gene            562..1107
                     /locus_tag="PKI33_01765"
     CDS             562..1107
                     /locus_tag="PKI33_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35754.1"
                     /translation="MEEQQLMEYFKFDASDLNANRNGTLTEKQKTRLNAELREARKSR
                     TRWAYFLFFLAAIGIAIALGIWFIPDSGLGMRIGFGIGFGLVWPALYGLVGFALLPPA
                     SYTDLDLASQTGRVNIVRVESRDPDTRAVSSRYDLYIGPRRFLVDSNIGHVLIQGDEY
                     TIYYLKNSNKIVSAEFISRAK"
     gene            complement(1149..1814)
                     /locus_tag="PKI33_01770"
     CDS             complement(1149..1814)
                     /locus_tag="PKI33_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35755.1"
                     /translation="MFKSKRRQIITPQSEHLKLVGTLAMLWGNDQFDTPPIERNSMVM
                     GMGLHDRGYGYLDNAPIGALRDEEWEGIARQTFTMECSDVIADTIVKYHFRRLAGYGS
                     SEIRKKLHADFTEGIHSQLERHNLSKALFDRTDRITDLLDTLSFEFCFDVPASGNVSV
                     FPRNGEETEVSIEYHVENGEINVHPWTFSVDEYRGYLIAYHAEGYPERLDPCILPYVL
                     KRK"
     gene            1936..2352
                     /locus_tag="PKI33_01775"
     CDS             1936..2352
                     /locus_tag="PKI33_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNM35756.1"
                     /translation="MSEVNDWNKKIIAEFRANGGKVGGNFEGRPLLLLHHKGAKSGQE
                     RVNPVACVQDGERYVVIASKGGAPTNPDWYYNVVANPLTTVEVGTETLKVQATVAEEP
                     ERTRLYNKMAEIMPGFNEYREKTTRVIPVIVLTPVK"
     gene            2361..2867
                     /locus_tag="PKI33_01780"
     CDS             2361..2867
                     /locus_tag="PKI33_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM35757.1"
                     /translation="MEAIHSERLTLRPLGLDDAGFIFELVNEPSFIQNIGDRGVRTLA
                     DAEKYIENSPLASYAKNGFGLLAVTLKETGEPIGMCGLIKRDALEDVDIGYAFLPKFW
                     LKGYAVEAARAVMGTAKALGLKRVVAIVDPANSGSIRVLERLGMSFEKMIRLSADDIE
                     LKLFSVAL"
     gene            complement(2922..3860)
                     /locus_tag="PKI33_01785"
     CDS             complement(2922..3860)
                     /locus_tag="PKI33_01785"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNM35758.1"
                     /translation="MENPTSDEKILAALAHASIILMFLGPLGAVIIWAIQRTKSKYVR
                     YHALQAMGYQTFSFWIWMFMMFFFMIILFGFGAIIGVLSSKSPSAMPPDFLFFIQPLF
                     MLVIFGMWGIIFLVGFIGAVFCGLGKEFRYPFIGSWLQTKILNAPTEEEAEKWEDLWV
                     ASICHTTAILMMWGMIMPIIVWFSQKDRSARLKFQSMQAFLYQLIVTVAYFISYAGFF
                     FVYVFGIAIFAASGVMADPSQTTMPAGFEVGFIIIMAIFMLFWLAWMILYPAYLITAG
                     VAAVRTLRGHDFKYPLLGKFLAKRMSPPESHEVIQA"
     gene            4049..4453
                     /locus_tag="PKI33_01790"
     CDS             4049..4453
                     /locus_tag="PKI33_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35759.1"
                     /translation="MNYRLLATINAVLLAVVGLIFLIVPVLVLNFFNTETYTATSLVA
                     RFLGSAMLLAGMFIWVAKDLTDVGTVRTMTIMLLVSSLVSFILTLVGMVGVDVIRANG
                     WIPLVVQILFVFGYGYLVSGVTITATVQPKKK"
     gene            complement(4526..4786)
                     /locus_tag="PKI33_01795"
     CDS             complement(4526..4786)
                     /locus_tag="PKI33_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35760.1"
                     /translation="MRNGVCTACGSHNVYKSANNSWYQSGVKVAVLGDKVNEVFMTEA
                     YVCLDCRHLELVVEENAAAIFGKGKAFKDAVPASTNWKKVSG"
     gene            complement(4916..5062)
                     /locus_tag="PKI33_01800"
     CDS             complement(4916..5062)
                     /locus_tag="PKI33_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35761.1"
                     /translation="MNSTPEKTQVKHKRPSKGVRKHNRKVKQEARRVSVPGAEVKKSK
                     RSAS"
     gene            complement(5072..5302)
                     /locus_tag="PKI33_01805"
     CDS             complement(5072..5302)
                     /locus_tag="PKI33_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35762.1"
                     /translation="MNKSLFEEKWKLLRGEVNTRWSLMVEYDLLKVDKAEVKFDKFVT
                     MLQVKYGYTRQKAKEEIVKLWSDYESNHRSKN"
     gene            complement(5418..5672)
                     /locus_tag="PKI33_01810"
     CDS             complement(5418..5672)
                     /locus_tag="PKI33_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35763.1"
                     /translation="MQARTCSKCGSHNVYKNTGKNWHQDGVVLQMIAVDRFTYRFETD
                     AYLCLDCRYLEILVSETNTMYGDQKTLTEVVQASSNWAKA"
BASE COUNT         1514 a         1267 c         1454 g         1609 t
ORIGIN      
        1 ggcgaattat aaccaaagct ttaaaaagaa aaagcagtct cgtttgagac cgcttttctt
       61 tagctcgcca gtgattcaac gatagtcttt gcccgagagt aggcggtcca acgtcggttg
      121 acttcgtcac gtgcttcaac gcgggtatag ccatacttca cctgaagaat ggtaaggaat
      181 ttatcgagct tgtcatcggc tttatctacc ttcttaaggt cgtgctcagc gatgagactc
      241 caccactcca tggaatgccc gcggatgagt ttccattttt ggtcgaactt tgtcttcggt
      301 ttttgcataa cgcgctccta aaagagtata tgaatgattg gactcagttg gagttttcaa
      361 gatttcgttt ctggcttatt ccccagtcaa taggcaaaac aaaattgtca aactcgttcg
      421 cattcattgt actacgtgat gcataaaaaa gcagtgattt ttcaaaacga ttttcagaat
      481 tgataattgt tggatgtgac gctaatgtac cattgcgaat taagttgaga gagataaaaa
      541 tagttcaaag gagaagcacc catggaagaa caacagctga tggaatactt caagttcgac
      601 gccagtgact tgaacgccaa tcgtaatgga acccttaccg aaaagcaaaa gacgaggctt
      661 aacgctgaac ttcgcgaagc gagaaaaagc agaacacgct gggcatattt tctcttcttt
      721 ctagctgcta ttggcattgc tattgcactg ggtatttggt tcatccctga ttcagggttg
      781 ggaatgcgga tcggattcgg cattggcttt gggctggtgt ggcccgctct atatgggctg
      841 gtgggctttg cgcttttacc gcccgcctca tacacggacc tggatcttgc cagccaaact
      901 gggcgagtca acattgtgag agtagagtcg cgcgacccag atacccgagc tgtgagctct
      961 cggtatgacc tgtacattgg tcctcgcaga tttttggtgg attcaaatat tgggcacgtc
     1021 ctaattcaag gagatgagta caccatttac tatctcaaaa attcaaataa gattgtatcg
     1081 gctgagttta tctcgagggc aaaatagaac ttaatgaaaa ggtctcgctt gaaatagcga
     1141 gacctttttt attttctctt gagtacataa ggaaggatac atggatcgag ccgttcgggg
     1201 tagccctcag cgtgataggc gatgagataa ccgcgatatt catccacaga aaatgtccac
     1261 gggtgaacgt tgatctctcc gttttcaaca tgatattcaa tgcttacttc tgtctcttcg
     1321 ccgttgcgcg gaaagactga tacattgcca gaggcaggaa catcaaagca gaattcaaag
     1381 gaaagcgtat ccaagaggtc tgtgatgcgg tcggtgcggt cgaagagagc tttcgagagg
     1441 ttgtgtctct ctaattgaga gtggatgccc tctgtaaaat cggcatgtaa ttttttgcga
     1501 atctcagaac tgccataccc ggcgagacgt cgaaaatgat atttgacgat ggtatcggcg
     1561 atcacgtctg aacattccat ggtgaaggtt tggcgggcga tgccttccca ttcttcgtcg
     1621 cgtagagcgc caataggagc attatcaaga taaccatacc cgcggtcatg cagacccatg
     1681 cccatcacca tggagttgcg ttcgatgggt ggcgtatcga attggtcatt tccccataac
     1741 attgccaatg tgccaaccag tttgagatgt tcagattgcg gggtgatgat ttgtctgcgt
     1801 tttgatttga acatactaca tctccaaaat ttgactataa agtttcatat gcgattctat
     1861 caataaataa ttctcttaga gtaagagaaa ataaatagag accatcgaaa cataaaacac
     1921 cataggagaa ttttcatgag cgaagtaaac gattggaaca agaaaattat tgccgagttt
     1981 cgtgccaatg gcgggaaagt gggcggaaac tttgaaggca gacctttgtt gctcttgcac
     2041 cacaagggcg ccaagagcgg acaagaacgg gtgaacccgg ttgcctgtgt acaagatggc
     2101 gaacgttatg ttgtgatcgc ttcaaaaggc ggcgcaccga ccaaccccga ttggtactat
     2161 aacgtggttg ccaacccttt aaccaccgtg gaagtgggta cagaaacctt gaaggttcaa
     2221 gctactgtag cagaagaacc tgagcgcacc cgcctctata acaaaatggc agagataatg
     2281 cccggtttca acgaatatcg tgaaaagacg acacgggtta ttcctgtgat cgtgttgaca
     2341 ccagtgaagt aaattttctg atggaagcga tccattcgga aaggcttacg ttgcgcccgc
     2401 tgggcttgga cgatgccggg tttatttttg agttggtgaa cgaaccttct tttattcaaa
     2461 atatcggtga ccgtggtgtg cgaactttgg cggatgcaga aaaatacatc gagaacagtc
     2521 cgcttgctag ctatgccaag aacggattcg gcttgttagc agtcacgttg aaagagacgg
     2581 gcgagccaat tggtatgtgc gggttgatca agcgtgatgc cttggaagat gtggatatcg
     2641 gctatgcctt tttgccaaaa ttctggttaa aaggctatgc agttgaagcg gcgcgggctg
     2701 tgatgggaac ggcaaaagcc ctgggattga aacgagtggt tgcgatcgtg gaccctgcca
     2761 actcaggttc gatccgcgtg ttggagaggc tggggatgtc ttttgagaaa atgatccgct
     2821 tatcggcaga tgatatcgag ttgaagttgt tttccgtcgc tttataaaat aaaacggact
     2881 caggcgaaat gtctgagccc gtttttgatt catggatggg tttaggcttg gatcacttca
     2941 tgcgactcag ggggagacat tcgtttggca aggaatttgc ccaagagtgg atatttgaaa
     3001 tcgtgcccgc gcaaagtgcg cacagcggca accccagcgg tgatgaggta ggctgggtag
     3061 aggatcatcc atgcgagcca gaatagcata aagatagcca tgataatgat gaaaccaacc
     3121 tcaaacccgg caggcatagt agtttgagag gggtctgcca tcacaccgct ggctgcgaaa
     3181 atagcaattc caaaaacata tacaaagaag aacccggcat acgagatgaa atatgccaca
     3241 gttacgatga gttgatatag gaacgcttgc atcgactgga acttgagtcg ggcggagcgg
     3301 tctttttgtg agaaccagac gatgatcggc atgatcatgc cccacatcat caaaatggcg
     3361 gtggtgtgac aaatgctcgc gacccagaga tcttcccact tttcggcttc ttcttcggtg
     3421 ggggcgttga ggatcttggt ttgcagccaa gacccgataa atggatagcg gaactcttta
     3481 cccagcccgc aaaagacggc gccaatgaac cccactagaa aaatgatgcc ccacatgcca
     3541 aagatcacca gcatgaacaa aggttgaata aaaaagagaa aatcgggcgg catggcggat
     3601 ggagatttgc tgctcaatac gccgataatg gcgccgaacc caaataggat gatcataaaa
     3661 aagaacatca tgaacatcca gatccaaaag ctaaaggttt ggtagcccat ggcttgcagc
     3721 gcatggtagc ggacgtattt tgatttggtg cgttgaattg cccagatgat gaccgccccg
     3781 agcggaccta gaaacatgag gatgatcgaa gcgtgcgcca aagcggcaag gattttttca
     3841 tcagatgtgg ggttttccat gggtttcctc ctgcgttgaa ttgacttgat gatacctcta
     3901 ttctcaaaaa ataaaatgtg acattgggat taaatttgac gccatgcaag ggaaatgtaa
     3961 ggtttgcgct tcaaaattcc tttgcgctgt tttcggagcg ttgtatagta gctttgttct
     4021 acattcacac cgacttaagg agaatacaat gaattaccga cttttagcga ctatcaatgc
     4081 cgttcttcta gctgtagtag ggcttatatt tttgatcgtg cccgtgctgg tgcttaattt
     4141 ctttaacacc gaaacctaca cagccaccag tttggtcgcc cggtttttag gctctgccat
     4201 gcttctggct gggatgttca tttgggttgc caaagacctg accgatgtag gtaccgtccg
     4261 cacgatgacg atcatgttgc tggttagctc ccttgtgagc tttatcttga ccctggttgg
     4321 catggtgggc gtggacgtga tccgcgcaaa cggttggatt ccgctggtgg tgcagattct
     4381 gttcgtgttt ggctatggct atttggtttc tggtgtaacc atcaccgcaa cggttcagcc
     4441 gaaaaagaag taaacgcctt gttaaccaaa acggtcttgg gtatttcacc caagaccgtt
     4501 ttttgtttaa gcctgaaaac gaacattaac cggatacttt tttccagttc gtgctggcag
     4561 ggacggcatc cttgaaggct ttgcctttgc cgaagatggc ggcggcgttt tcttccacca
     4621 ccaattcaag atgacgacaa tcgaggcaga cgtacgcttc cgtcataaac acttcgttga
     4681 ccttatctcc gagcactgca actttgaccc cagattgata ccaggaattg ttcgcgcttt
     4741 tatagacatt gtgagaccca catgccgtac aaactccatt tctcattgct aaagtttctc
     4801 ctttgatatt gatactgtgt tcgattatat agagaagcag gggagcgggt attccctctt
     4861 tggtcatgtg gaaaataaaa acggtctcat cggataaacc tgagaccgtc ctgttttacg
     4921 aagctgaacg cttactcttt ttgacctctg cccctggcac actgacgcgt cgcgcctctt
     4981 gcttgacctt gcggttgtgt ttgcgtaccc ctttggaggg acgtttgtgc ttgacctgtg
     5041 ttttttctgg ggttgaattc atgtggctcc tttagttttt acttcgatga ttcgattcat
     5101 aatctgacca aagtttgacg atctcttctt tggctttttg gcgggtgtac ccgtatttga
     5161 cttgcagcat ggtgacgaac ttatcgaact tcacctcagc tttatccact ttgagcaggt
     5221 cgtattcgac catcaaactc caacgggtgt tgacctcccc gcgcagtagc ttccattttt
     5281 cttcaaacag ggatttgttc ataaggcgct cctcctcaag aaagcgcgtt tttagggaat
     5341 acgggttggg agttttgtga tttcgtgccc atccatctcc cccatgaata ggaatgaaaa
     5401 agattcaaac tcttatgtca ggcttttgcc caattgctac tggcttgaac gacttctgtc
     5461 agtgtttttt gatctccgta catggtgttt gtttcagaga ccagaatttc caaatacctg
     5521 caatcgaggc agagataagc atctgtttca aagcgatagg tgaaacgatc tacggcgatc
     5581 atttgcagga caaccccatc ctgatgccag tttttacctg tatttttata gacattgtgg
     5641 gaaccacatt ttgagcatgt tcgagcttgc atggaaatct ccttctcaga tttgagtgta
     5701 tccgtctcgc ttaatagggc gagtaaggtt ttctggaagg tatgttgatg tgattgcatc
     5761 ccccgttacc gtctagttgc ctgatttcat tgtacccttt tctgggaata aagatagaca
     5821 agaaaaaaac ggtctcgcgg atat
//
LOCUS       DAOREF010000054         5817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7413_length_5817_cov_4.473100, whole genome shotgun sequence.
ACCESSION   DAOREF010000054 DAOREF010000000
VERSION     DAOREF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7413_length_5817_cov_4.473100"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKI33_01815"
     CDS             <1..498
                     /locus_tag="PKI33_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNM35764.1"
                     /translation="VDGCRMRGLTLPHLRLEPGRSLVAQAGVAMYRVGAIKKRGHKTW
                     LLTDGGMADNPRFALYGARYSCLTVSGPGGERSERVSIAGPYCESGDIVIEDLPMPKV
                     KEGDLIAIPVAGAYHLSMSSNYNGARKPAVLWLEEDHVQLIRQRETVQDLFEADLKLA
                     VLDKL"
     gene            complement(469..5541)
                     /locus_tag="PKI33_01820"
     CDS             complement(469..5541)
                     /locus_tag="PKI33_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM35765.1"
                     /translation="MPKIQNEKFVSSLDEWRADLLNLILGGIFVFWVLAFIGSIRNVI
                     DLYNKSQSLLNNPKLVAISVIVFYGIVTTLVGYVTFNRRLNVNLRAGILLFSLYALGT
                     SGMLLTSFSGDGRVLLFAFVIFSGIFFNLRYSLAAFFVTLLTLVVIGYLQISGLMVVP
                     PEWQVNAANPGAWFSGGLVLIVLSVAGIYSIAYLLQSFEASLKETQEALQREQRLGGV
                     LRTVSEINQIIVRAQDPEKMLHDACELLVAGQGYSFAWVGLLDEDMVTLRHIAHAGGD
                     VDESEFTTRLDLESGLTCAIQAIRTRSFFIVNPSDRNGDPCEKCPRIGKFPNRSAISL
                     PLQRDAKIFGVLVVDHTELAAAFDEQEISLLVELAGDLSYALENLEASKRLDTIARYQ
                     TLLNEVTQTALSAFDLDTLLKDFIEKLEKALDADGYYFAMWDEARGVPSKFIFSQKFH
                     DIFVTPPLLEPDDKFFTRAILEEKRILIIQNVMDTPYINPAFAEQFNVQSAMGIPLIA
                     DQMRIGALVLGYSDQHVFTSEEVKLADQSMNQFALAILKVKLNDEMRAKAKELEQLYT
                     AAQDMTASLMDPPALLKEMARHMAEALDVTSTYITSFDLQVMSMKVVAEYWGKESAPA
                     ELKSDLGRDYPSADYATVLKAMMEGRSLVLHAGQPNLTSEEYQQFVDYHIETMMFVPI
                     YSRGRLLGTVELWESRKRREFSQAEIRLANAMTSHAANIIENSTLFALTRKRESELSA
                     LLSVTRAISSSLQLPDVLREATTTLARMMRVDFCSISDYLPESNLLITRAIYSAEDDV
                     SEQGDIGKEFSLEHYPATLYVLQTGKPLVTRMDDAGADPAEIQQLKRDGMFSSLLIPL
                     RVRGYSLGLAELFTSDESRHFQQEEIQFASTLADQVAVAIENAYLYERLEQREAYFRA
                     LVENSTEGVAILDQQGIVRYLAPAEERLTGYSVDEIVGGSAFKYIHPDDLSKVLDMFQ
                     QGIENPGVVLSVQYRLKMKNGEWGHFEVSGSNMLGIPDIAGIVVNYRNITERVRTEKA
                     IEESEERYRTIFESAGIPIWEDDYSEFMASIEELKRNGITDFRKYFTEHPEFVERAAS
                     MIRITGVNAAAMSLMGATTKSQLIGALDSVLFSQPSNRFIDDVVALTDGRRHIEQESE
                     LFTLSGERRDVWVTINLPERAKGYERVLVSTLDITERKQIENALIESQGRLLGIVTTA
                     PNGIITVDANIRIILMNPAAEKIFGYLAEEVLGQSLNMLLPERFREHHAKYIQAYSAS
                     GVSHRKLGRYDSLYGIRKNGEEFPMEAFISRYEMEGEKFFTVILQDITVRKQDEDNLR
                     RRAEELQTLVIVSSALRSAVKVDEIIPLVARYSAEIVGGTYSTVYLLDQSSDALVSPG
                     WYSVRNGEDIKTTTAPLLRNASGNGITGYVASTGNIYITEDLRNDPKASILPDEIETL
                     RDAHSGISLPLLSQEMVIGVLHVRLTEKHVFNETEIRLLTAIAEMAGNALHRTKLYEQ
                     TVQQSEELAQAYDNTLSGWARALELRDELTEGHTRRVTELTLELARAMNISESELVQI
                     RRGAILHDIGKMGIPDAILNKPGPLTAHEIRIMQMHTQYAYEMISFIPFLKPAIDIPY
                     CHHEWWNGNGYPRGLKAEEIPLSARIFAVIDVWDALTSDRPYRAAWSTENALKYIQDG
                     SEKQFDPKVVDAFLKLIENRKL"
BASE COUNT         1644 a         1363 c         1209 g         1601 t
ORIGIN      
        1 gtagatggtt gccgaatgcg tgggcttaca ctcccccatt tacgtcttga accaggtcgt
       61 agcctagttg cgcaagctgg tgtggcaatg tatcgagtgg gggctataaa aaagcgcggt
      121 cacaaaacct ggctacttac agatggaggc atggcagata acccgcgctt tgcactttac
      181 ggggcaagat attcttgttt aacggtatca ggcccgggcg gggaaagaag cgagcgtgtc
      241 tccattgcgg gaccgtattg tgagtctggg gatattgtga ttgaagattt gccgatgcca
      301 aaagtcaaag agggagattt gattgcgatc cctgtagcgg gggcatacca tttgagtatg
      361 tcatcgaatt ataacggcgc gcgcaaacct gctgttttat ggctggagga agaccacgtg
      421 caattgatcc gacaacgtga aacggttcag gacttgtttg aagcggatct aaagcttgcg
      481 gttctcgata agctttagga aggcatctac tactttaggg tcaaactgtt tttcggaacc
      541 gtcttggata tatttcaacg cattttctgt agaccaagcc gcgcggtatg gacggtctga
      601 tgttagtgca tcccaaacat cgataactgc aaagattcgg gcagagagtg gaatctcttc
      661 cgctttcaac ccgcgtggat acccgttgcc attccaccat tcatgatgac aataagggat
      721 gtcgatagct ggttttagga acgggataaa tgagatcatt tcgtatgcat attgcgtatg
      781 catttgcatg attcgaattt catgcgctgt caatggacct ggtttattga gaattgcatc
      841 tggaataccc atcttcccaa tatcatgcaa aatagcacca cggcgaattt gcaccagctc
      901 gctttccgaa atattcatag ctcgtgccag ttccagcgtt agttcggtta cgcggcgagt
      961 atgcccctct gtcaactcat cgcgcaattc caatgcacgt gcccagccag aaagggtgtt
     1021 gtcgtatgct tgagccagtt cttcagattg ctgcacggtc tgttcgtaaa gttttgtacg
     1081 atgaagagca ttgcccgcca tttctgcaat ggctgtaagt aagcgaattt cagtctcatt
     1141 gaaaacatgc ttctctgtta gacgcacatg cagaacccca ataaccattt cttgagaaag
     1201 cagtggaagt gatatgccac tatgggcatc acgaagcgtc tctatttcat ctggcaagat
     1261 agaagccttt gggtcattac gcaaatcttc ggtaatatag atattgcctg tactggctac
     1321 atatccagtt atgccgtttc cagaagcatt ccgcaatagc ggtgctgtgg tggtcttgat
     1381 atcttcccca tttcgtaccg agtaccatcc cggcgagact aaggcgtcgg aagattggtc
     1441 taaaagatag acagtgctat aggtgccacc aacgatctca gcagaatagc gtgccaccaa
     1501 tggaataatc tcatccactt ttacggctga acgaagagca gaagagacaa tgaccaaggt
     1561 ttgtaattct tccgctcggc ggcgcaaatt atcttcgtct tgtttgcgaa cagtaatatc
     1621 ttgcaagatt acagtaaaga acttctctcc ctccatttca taacgtgaaa tgaaggcttc
     1681 catgggaaat tcttcgccat tcttgcgaat accatataac gaatcatatc taccgagttt
     1741 acgatgggaa actccgcttg cactataggc ttgaatatac tttgcatgat gttcccggaa
     1801 acgttctggg agcaacatat ttaggctttg ccctaaaact tcttctgcca gatagccaaa
     1861 tattttctca gccgctgggt tcatcaagat aatgcggata ttggcatcga ctgtaataat
     1921 gccattgggc gctgtagtca caatacccaa aagcctgccc tggctctcga tgagagcatt
     1981 ctcaatttgt tttctctctg taatatctaa tgtactaaca agaactcgtt catacccttt
     2041 tgcacgttca ggcaaattaa tggtcaccca cacatcacgg cgctcgccag acaaagtaaa
     2101 caattcgctc tcttgctcaa tatgtctacg tccatctgtc agtgccacaa catcgtcaat
     2161 aaatctgttt gatggctgag aaaataaaac actatcaagt gcaccaatta gttgtgattt
     2221 tgtggttgcg cccattaaac tcattgcagc agcattaacc cccgtaatgc ggatcatact
     2281 tgcagcacgc tcgacaaatt ccgggtgttc agtaaaatat ttacggaaat cggtgatccc
     2341 attccgtttt aattcttcaa tacttgccat aaattcagaa taatcatctt cccaaattgg
     2401 aatcccagca gactcaaaga tagtgcgata tctttcctca gactcttcaa tggctttttc
     2461 agttcgaacc cgttctgtaa tatttcgata attaactaca attccagcaa tatctggaat
     2521 acccaacata ttgcttccgc ttacttcaaa atgcccccac tcaccatttt tcattttcaa
     2581 tcggtattga acagaaagga cgacaccagg attttcaata ccctgttgaa acatatctag
     2641 cactttggat aagtcatcgg gatgaatata cttaaatgcg cttccgccaa cgatttcatc
     2701 aacagaatat cccgtcaatc tttcttccgc cggcgctaaa tagcgtacga tcccttgttg
     2761 gtcaagaatt gcaacgcctt ctgtggaatt ttccaccagt gcgcggaagt acgcttcacg
     2821 ttgctccaat cgttcataca aataagcatt ttcaatggca accgctactt gatctgctaa
     2881 tgtgctggca aattgaattt cctcttgctg gaagtggcgg ctctcatccg atgtgaataa
     2941 ctctgccaag cctagcgaat atccacgaac tcgcaacgga attaacaaag atgagaacat
     3001 tccatccctt ttcagttgtt gaatttcggc agggtctgcg ccggcatcgt ccatacgggt
     3061 gaccagaggc ttcccagttt gaagcacata caaggttgcc ggatagtgtt ctaatgaaaa
     3121 ttcttttcca atatctccct gttcgctaac atcatcttcg gctgaataaa tagcacgagt
     3181 gatgagcaaa ttactttcag ggagataatc tgaaatggaa cagaagtcca cgcgcatcat
     3241 acgtgcaaga gtagtggttg cttcacgaag aacgtcaggg agttgcaagc tggatgaaat
     3301 cgcacgagta accgataata aggcacttaa ttcactttcc ctttttcgag ttagtgcaaa
     3361 cagggtcgaa ttctcaatga tattcgcagc atgactagtc atggcattgg caaggcgaat
     3421 ctctgcctga ctaaattctc gacgcttacg gctctcccaa agttcgaccg tgccaagtaa
     3481 tcgtccgcga gaatagatcg gaacgaacat catggtttcg atatgataat caacaaattg
     3541 ttgatattct tctgaggtca agttaggttg cccagcatga agtaccagag acctgccttc
     3601 catcattgcc ttcaaaacag tggcatagtc agcactgggg taatctctgc caaggtcaga
     3661 cttaagttca gcaggcgcag attctttgcc ccagtattct gccaccactt tcattgacat
     3721 cacttgtaaa tcaaatgaag tgatgtatgt gcttgtgacg tccaaggctt ctgccatatg
     3781 acgcgccatt tctttcagca atgccggcgg atccatcaag ctggcagtca tatcttgagc
     3841 agccgtgtaa agctgttcta actcttttgc tttggcacgc atctcgtcgt ttagtttcac
     3901 tttaagaatg gcgagagcaa actgattcat ggactggtca gctagtttca cctcttctga
     3961 ggtaaataca tgttgatcac tatagcccaa aactagggca ccaattcgca tttggtctgc
     4021 aattaatgga atacccatgg cagattgcac attaaactgt tctgcaaacg cagggttaat
     4081 ataaggcgta tccatgacgt tttgtatgat aagaatgcgc ttttcctcca aaatagcgcg
     4141 ggtaaaaaac ttatcatctg gctctaaaag cggcggagtc acaaaaatat cgtgaaattt
     4201 ttgagaaaaa ataaacttgg aaggaacacc tcgcgcctca tcccacatcg cgaaatagta
     4261 tccatcagca tctaaagctt tctcaagctt ttcaataaaa tctttcaaca acgtatcaag
     4321 gtcaaatgca gataaagctg tttgtgtaac ttcattcaat agagtttgat accgagcaat
     4381 tgtatccagt ctctttgatg cttcaaggtt ttcaagggca taggataaat cacctgccaa
     4441 ctccaccagc aaggaaatct cttgttcatc aaaggctgcc gctagttctg tatggtcaac
     4501 aaccagcaca ccaaatattt tcgcatctcg ctgtagaggt aaagagatcg cagacctatt
     4561 cgggaatttc cctatgcggg ggcatttttc acacgggtct ccgttacggt ctgatggatt
     4621 aacaataaaa aatgaacggg ttcgaatcgc ctgaatggca caggtcagcc cgctctcaag
     4681 gtcgaggcga gtagtaaatt cgctttcatc cacatcacct cctgcatgag caatatgccg
     4741 aagtgtgacc atatcctcat ccaacaaacc cacccatgca aaggagtacc cttgcccagc
     4801 aacaagaagc tcacaggcgt cgtgaagcat cttttctggg tcttgtgctc gcacaataat
     4861 ctgattaatt tcactgaccg tacgcagaac cccgccaagg cgctgctcac gttgtaaagc
     4921 ttcttgggtt tctttcaagc tggcttcaaa agattgtaag aggtaagcta tcgaataaat
     4981 cccagcgaca cttaatacaa tcaacaccaa cccaccactg aaccatgccc cagggttggc
     5041 ggcattcacc tgccattcgg gcggaaccac catcagccca gaaatttgca gatatccaat
     5101 gaccactaat gttagcagag ttacgaaaaa ggcagccaaa ctatagcgta gattaaagaa
     5161 tatccctgaa aagattacaa acgcaaataa aagaactctg ccatccccac tgaacgaagt
     5221 gagcaacatt ccgcttgtgc ccaaagcata taaactaaaa agtagtattc ctgcacgcag
     5281 gttcacattc aatctacgat tgaatgtgac ataaccaacc aatgtggtca ctatgccgta
     5341 aaacacaata accgaaatcg caaccagttt tgggttattc agtaggctct gacttttatt
     5401 gtatagatca atgacgtttc gaatgcttcc aataaatgcc aagacccaaa aaacgaaaat
     5461 tccacccaag attaagttca aaaggtcagc ccgccattca tctaacgatg aaacaaactt
     5521 ctcattttga atcttgggca tagcaaaaaa tgcgtctttg agtctttgat acataatgat
     5581 ttaggctatt tttaaattat agttgcattt tttgcctgaa acctttcaga gttagtacgg
     5641 ttttgcccaa aaacgcccct tctgcaagcc tttttcatta aatattatga tatttatcac
     5701 atggacaggt ggaaatacag acaggtatac tacgccaatc tttttagaga atttttgtct
     5761 gcaatccaac tcgaggagaa atcatgtccg ctagggattc tgttccttct tctaaat
//
LOCUS       DAOREF010000055         5798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7452_length_5798_cov_5.070869, whole genome shotgun sequence.
ACCESSION   DAOREF010000055 DAOREF010000000
VERSION     DAOREF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7452_length_5798_cov_5.070869"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..409
                     /locus_tag="PKI33_01825"
     CDS             188..409
                     /locus_tag="PKI33_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNM35766.1"
                     /translation="MRRMFGFMIGIFTGALVGSVIALLLAPESGENLRGQLRNRGQNF
                     LNDVRHSADARRIELRNHLESLRSSREEF"
     gene            complement(406..1572)
                     /locus_tag="PKI33_01830"
     CDS             complement(406..1572)
                     /locus_tag="PKI33_01830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM35767.1"
                     /translation="MRLLFVTDARSPISKNWIRHFIERGDEVFIASTFEWDVEGISSE
                     RTEFTPVAFSSAKKRTSAPSTASSRTLSLRTKIRQWLGPVTIPAAAFKLRAFIREVQP
                     DIVHAMRIPYEGMLTAEALQGLKARPTFLASIWGNDFTLHAPSTPLMRYYTRKVMNSV
                     DGLHADVERDVNLAHEWGLSVEKPTLVTPGNGGIRSDVFYPPKELVKSPIIINPRGVR
                     PYVRNDSFFKAIPLVLAKYPDAKFLCVGMQNEPQAMRWMQEYGIENSVELLPTSPHEK
                     MGEVFRGAQIVVSPSIHDGTPNTLIEAMACGCFPIAGDLESIREWISHGQNGLLFDSN
                     NSQSIADAMLLGLEREDLRTEAAGLNKKIISARAEFSTNMNKVEKFYSVLRGTN"
     gene            1637..2011
                     /locus_tag="PKI33_01835"
     CDS             1637..2011
                     /locus_tag="PKI33_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM35768.1"
                     /translation="MSAGRILIVEDNMDTYELVRFILEKNGFETFLAMNGRDGVNAAT
                     KQRPDLIIMDLSMPEMDGWTAMRLIKSDPAISSIPMIALTAHALPSDRKRAFDAGCDE
                     YITKPMDLIDLLDTVKSWIQKV"
     gene            2096..3391
                     /locus_tag="PKI33_01840"
     CDS             2096..3391
                     /locus_tag="PKI33_01840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNM35769.1"
                     /translation="MKQICVVGVGYVGLVTAACFADLGNRVVALDVNEERVANLKKGI
                     MPIYEPGLDELVKRNVKAGRITFTSSYTEALKGAEYAFIAVGTPSGSDGSADLQYVAA
                     AATSIAKNMTAPLVLINKSTVPIGTGDWVADIVKGAQPKPIDFAVVSCPEFLREGSAI
                     GDFMNPHRTVIGSLDRDAANKVAQLHLPLRAPIVITDLRTAEMIKYASNAFLATKISF
                     INELADLCERVGADVKEVAAGMGYDARIGRHFLDAGLGWGGSCFPKDVEALAFMAKEK
                     GLEPKILNSVMDVNYDRRKEAVKVVENMVGGSLKGKTIGLLGLAFKPNTDDMRDAPSI
                     DISAELIEAGAKVRGYDPVAMSVARPILPAVEMYDEPYKLAEGCDALMIITEWNEFKQ
                     LDLDKVKGLLKSPIIYDGRNIYEPGRMKDMGFTYRAIGR"
     gene            3481..4383
                     /locus_tag="PKI33_01845"
     CDS             3481..4383
                     /locus_tag="PKI33_01845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM35770.1"
                     /translation="MTNTPPVCNYEGSDYQQSFWDQGGRAYEDQSEAIALRRMLPKSG
                     KLMLEVGAGAGRNTPRYAGFDRIVVMDYSTTQLAQAQERLGRSDKYIYVAADVYRLPF
                     RNGLFDGATMIRVLHHMADAPKALGQIQGTLQPGATFILEFANKLNLKAILRYWLGRQ
                     AWSPFTLEPVEFVKLNFDFHPNAIRQWLGNLGFNIEKTLTVSHFRLGLLKRTIPSKLL
                     AAVDGLFQPTGALFQLTPSVFVKAVLPGASSADIPLNVIDLFKCPECNSGGLQEKTDH
                     VFCPSCKAKWSVKDGIYDFREKIS"
     gene            complement(4420..>5798)
                     /locus_tag="PKI33_01850"
     CDS             complement(4420..>5798)
                     /locus_tag="PKI33_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNM35771.1"
                     /translation="QREQAKREVDQLYAAEQARRHGAETLARSMRQLSSLKNVEQLPE
                     QILEQLNQALPYDRGVLFMEDVNGEPQIQAHRGMPANADMASFRLGMNGSDFYTTVSR
                     KGETLVVSDVNAVENWSQPEWLVRDRAWMGVPLYLKDNVIGLLMVSRADRPFTHDDGL
                     LATTFASQATVALENARLYHEVTGINQMMERMVSERVAELNETYKTLEKHDKNKSAFI
                     QVAAHELRTPLTVVKGFLGMLQNDATVRSNSMLSDAVEGVLRGTNRLHQIVNSMLDVV
                     RLENQVITPHLEAVSLGLILRLIHKDYVDDLATRNLTFTLDDSVKTIPPMLADSELVK
                     KALDQVIVNAIKFTPDGGSIYISAWVVEDPKRGKMAEIAIKDSGIGIDPEHHKVIFEK
                     LYQMGEVQLHSSSRTNFKGGGAGLGLAIASGIVRALQGTIWVESPGKDEEKYPGSTFF
                     IRLPLLKE"
BASE COUNT         1513 a         1441 c         1352 g         1492 t
ORIGIN      
        1 aagcgatggg cgtcaccatc gaagacagca aagacggcac cacctggaag tggagctagt
       61 ttcgtagtgt gacagtcaat cagttgaata gtaaaacttc cgagaaatgt ttctcggaag
      121 tttttatttg taacaacttt cagatgtata attcaattat caaaacgaat tttcaaggag
      181 aaattccatg cgtagaatgt ttggctttat gatcggcatc ttcaccggcg cgttagtcgg
      241 ctctgtcatt gcacttcttc tcgcccctga atccggtgaa aacctgcgcg gacaactccg
      301 taaccgcggg caaaacttcc tcaacgatgt gcgtcactcg gcagatgccc gccgcattga
      361 attacgcaat cacttagagt ctcttcgctc ctcaagagaa gaattctagt tcgtacctct
      421 taatacagaa tagaacttct ccactttatt catatttgta ctaaactctg cacgtgcaga
      481 gataattttc ttattcagac ctgcggcttc ggtgcgcagg tcttctcttt ctagacctaa
      541 taacattgca tctgctatcg attgcgagtt gtttgagtca aacaacaaac cgttttgccc
      601 atgtgaaatc cactcacgaa tggattcaag gtcacctgct atggggaaac atccacacgc
      661 catggcttcg atcagcgtgt ttggcgtccc atcatgaata ctgggggaaa ccacgatctg
      721 tgcaccacga aacacctctc ccatcttttc atgaggagag gtaggcaaca actccacaga
      781 attttcaatt ccatactctt gcatccacct catggcttgt ggctcatttt gcatacctac
      841 acataagaac ttcgcatcag gatattttgc cagcaccaag ggtatcgcct taaaaaacga
      901 atcattccgc acatagggtc ttactccacg agggttgatg atgattgggc ttttcaccaa
      961 ctcttttggc ggataaaaaa catcgcttct aattccgccg ttaccgggtg tcaccaacgt
     1021 aggtttctct acactcaagc cccattcatg cgctaggttt acatcccgct ctacatctgc
     1081 atgcaaacca tctacagaat tcatcacctt gcgagtgtaa tacctcatca gcggtgtaga
     1141 tggggcatgc agggtaaagt cattgcccca aatggaagcg agaaatgtag ggcgagcctt
     1201 cagcccttgt agggcttcgg cggtcaacat accttcatac ggaatgcgca tggcatgaac
     1261 aatatctggc tggacttctc taatgaaagc ccgcaatttg aacgcggcag cgggaatggt
     1321 aacaggacca agccactgcc gaattttggt gcgcaggctc agggtccggg aagaggcggt
     1381 ggagggagca gaggtacgtt ttttagcaga gctaaatgcc acaggggtaa attcagtccg
     1441 ttcggaggag atgccctcca cgtcccattc aaaagtagaa gcgataaaaa cctcgtctcc
     1501 gcgctcgata aaatgacgaa tccagttttt tgagatgggg gaacgggcat ctgtgacaaa
     1561 tagcaggcgc ataaggtgat tatacattgt aaaaatattg ggctatcgca acctactttg
     1621 tagtatattt aagcgcatga gcgcaggacg cattcttatt gttgaagata atatggatac
     1681 ctacgagctg gtacgtttta ttctggagaa gaacggtttt gaaaccttcc tcgctatgaa
     1741 tggacgagac ggcgtcaatg ctgccactaa acaacgccca gacttgatca ttatggacct
     1801 ttccatgcct gaaatggatg gttggaccgc catgcggttg atcaaaagcg accctgccat
     1861 atcttccatc cctatgatcg ccctaactgc tcatgccctg cccagtgacc gcaaacgtgc
     1921 gttcgatgca ggctgcgatg aatacatcac caaacccatg gacctgatcg atttgttgga
     1981 taccgttaag agttggattc aaaaagtata aatcaggcta gggaaaataa atggcttata
     2041 gcaatataca aagtctcata ttaaagagcc aaatcttcaa aaaaggaaat acaccatgaa
     2101 acaaatttgt gtcgtcggcg tgggctacgt cggtctcgtg accgctgcct gttttgccga
     2161 tttaggtaac cgcgtggttg ccttggatgt caatgaagaa cgcgttgcca atctcaaaaa
     2221 gggcattatg cccatttacg aacccggctt ggatgaactt gtcaaacgca atgtaaaagc
     2281 agggcgtatt acgttcacaa gctcctatac agaagcgctg aaaggtgcag aatacgcctt
     2341 cattgctgtg ggtacgcctt ctgggtctga tgggtctgct gatttgcaat atgtggcagc
     2401 cgctgccact tccattgcga agaacatgac cgcaccattg gtcctgatca acaagtccac
     2461 ggttccaatt gggactggtg actgggtcgc cgatattgtg aaaggtgccc agcccaaacc
     2521 gatcgatttc gccgttgtct cttgccccga gtttttacgt gaaggctccg ctattggcga
     2581 cttcatgaat ccgcaccgca ccgtgatcgg ttccttggac cgtgatgctg caaacaaggt
     2641 tgctcaattg cacttacctt tgcgcgcccc tattgtcatc accgacttgc gcacggcaga
     2701 gatgatcaag tacgccagca atgcttttct cgccaccaaa atttccttca tcaatgaact
     2761 cgctgacttg tgcgagcgcg ttggtgcaga tgtaaaagaa gttgcagcag gcatgggcta
     2821 cgatgcccgc atcggacgtc acttccttga tgcaggtctt ggttggggcg gctcttgctt
     2881 ccccaaagat gtagaagccc tcgccttcat ggcaaaagaa aagggactgg aaccgaagat
     2941 tctgaacagc gtgatggatg ttaactacga ccgccgcaag gaagctgtga aggttgtgga
     3001 aaacatggtc ggcggcagcc tcaaaggcaa gaccattggt ttgcttggct tggctttcaa
     3061 acccaatacc gacgacatgc gtgatgcgcc ctccattgat atctcggcag agttgattga
     3121 agcaggcgcc aaggtccgcg gatatgaccc ggttgccatg agtgtggcac gcccgattct
     3181 cccggctgtg gaaatgtacg atgaacctta caaacttgcc gaaggctgcg atgcgttgat
     3241 gatcatcacc gaatggaatg agttcaaaca gctcgacctc gacaaagtga agggtttact
     3301 caaatccccg atcatttacg atggacgtaa tatctacgaa cccggtcgta tgaaagacat
     3361 gggcttcacc taccgcgcaa tcggacgcta attttttagt tcatttgttt ggtagtcgag
     3421 tagtcgggta caatctgacg gctcgactac caatttttta aacacaaaaa gaacagaatt
     3481 atgaccaata ctcctcctgt ttgtaactat gaaggctccg actatcagca atccttttgg
     3541 gatcaaggcg ggcgcgccta cgaagaccaa tctgaagcca ttgcattaag acgcatgttg
     3601 cccaaaagcg gcaagctgat gttggaagtg ggagccggtg ccggacgcaa cacgccacgc
     3661 tatgccggtt ttgaccgcat agtagtcatg gattattcaa ctactcaact cgctcaagct
     3721 caagagaggc tgggtcgctc cgacaaatac atttacgtcg ctgcagatgt ctaccgcctc
     3781 cccttccgta atggactatt cgatggcgca accatgatcc gcgtgctcca tcacatggca
     3841 gatgcgccca aagcgttggg gcagattcag ggaacactac aacccggggc aacttttatc
     3901 cttgagtttg caaacaagct taacctcaag gcgattctgc gctattggct cggcaggcag
     3961 gcatggagtc cgttcacgtt ggagccggtg gagttcgtca aattgaactt tgactttcac
     4021 cccaatgcta ttcgtcagtg gttgggaaat cttggtttca atattgaaaa gaccttaacc
     4081 gtctcacatt ttcggcttgg tttgctcaaa cgaactattc cttctaaact gctcgccgct
     4141 gtggatggtc tctttcagcc aacaggtgcg ctcttccaac ttacgccaag cgtattcgta
     4201 aaagcagttt tgcccggtgc ctccagtgca gatattccgt taaatgtcat tgacctcttc
     4261 aaatgtcccg aatgtaacag tggaggattg caagagaaaa cagaccatgt attttgtcct
     4321 tcttgcaagg ctaagtggtc tgtcaaggat gggatttatg attttagaga aaagataagt
     4381 tagaaaataa aacgcgctct acattgagcg cgttttttgt tattccttca acaacggcaa
     4441 gcggatgaag aaggtgctgc cggggtattt ctcttcatct ttgccggggc tttccaccca
     4501 aatcgttccc tgcaaggcac ggacaatacc agaggcaatg gcaagtccca acccagcacc
     4561 tccgcccttg aaattggttc ggctagacga atgtaattgc acctcgccca tttgatataa
     4621 cttttcaaaa ataactttat ggtgttcggg gtcaatacca atcccagaat ctttgatggc
     4681 gatctctgcc atcttgccgc gtttgggatc ttccactacc caagctgaga tataaatcga
     4741 gccgccatcg ggtgtaaatt tgatagcatt gacgataact tgatccagtg ccttcttcac
     4801 aagttcagag tccgctaaca ttggagggat cgtttttaca gagtcgtcca atgtaaaggt
     4861 caagttacga gtcgcgagat catccacata atctttgtga atgaggcgta agatcagccc
     4921 caaactgacc gcttctaaat gtggcgtgat gacctgattc tcaaggcgca ccacatccaa
     4981 catgctgttt acgatctgat gcaaacggtt cgtgccgcgc aagacccctt ctacggcatc
     5041 ggaaagcatt gaattcgaac ggacggtggc atcgttttgc aacatcccaa gaaaaccctt
     5101 caccaccgtc agaggtgtgc gcagttcatg tgctgccacc tgaatgaacg cagatttgtt
     5161 cttatcatgc ttctcaagtg ttttataggt ttcattcagt tccgcaacac gctcggaaac
     5221 catgcgttcc atcatttgat taatgccagt cacttcgtga tacaaacggg cgttttccaa
     5281 agcaacggtt gcttgagaag caaatgttgt agctagcaaa ccatcatcgt gagtaaatgg
     5341 tctatcagca cggctgacca ttaagagccc gatgacatta tctttaaggt ataaaggcac
     5401 gcccatccaa gcacgatcac ggacaagcca ttcgggttga gaccaatttt caaccgcgtt
     5461 gacatcgctc accacaaggg tctcaccttt acgtgaaaca gtcgtgtaaa aatcactgcc
     5521 attcattccc aaacgaaacg acgccatatc tgcattagct ggcatgccgc gatgggcttg
     5581 aatctgtggc tcaccattta catcttccat gaaaagcacg ccacggtcat acggcaaggc
     5641 ttgattcaat tgttccagaa tctgttctgg taactgttca acatttttta gtgaggataa
     5701 ttggcgcatc gagcgtgcca acgtctcagc accatgacgc cgcgcctgtt ccgccgcata
     5761 aagttgatcc acttcacgtt tggcttgctc acgttgat
//
LOCUS       DAOREF010000056         5764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7514_length_5764_cov_4.807497, whole genome shotgun sequence.
ACCESSION   DAOREF010000056 DAOREF010000000
VERSION     DAOREF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_5764_cov_4.807497"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..788)
                     /locus_tag="PKI33_01855"
     CDS             complement(123..788)
                     /locus_tag="PKI33_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNM35772.1"
                     /translation="MKRFIAIAGNIGVGKSTLVQMLCNQMGWDPFYEPVTQNPYLADF
                     YKDMSSWSFHSQVFFLTHRLRSHLNLAQHPNSVIQDRSVYEDAEIFAHNLYQQGNMTD
                     RDYQTYRDLYETAVQFLPPPDLVIYLRASVDTLLNRINHRGRDYERTIAPDYLQSLND
                     LYEGWIDNFTLCPVLAVPADDLDYVAHNGHLRLIVSKIEAKLTGKEEVVFEPEEVARA
                     AED"
     gene            complement(972..1742)
                     /locus_tag="PKI33_01860"
     CDS             complement(972..1742)
                     /locus_tag="PKI33_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNM35773.1"
                     /translation="MNTKSTRSKKSTPAVIEIEQEEPVIESTPDSEDFITFKRSHFYS
                     VLVVLAFAVGLLLGFVIWGRGSNTVTVAADPAAAANNAAAQQPSGEIAEAPVTPQPQY
                     TRYDIPTEGYPSLGPDDAPITIVEFSDFQCPFCRRFHSETYQALLDAYPGQIRFVYRN
                     LPLTSIHPDAMPSAIASLCANDQNVYWEYHDKLFSSETLTRDTFIQYATDLNLNVDEF
                     SACLDSGKHDENIQQDMDFALTLAFNPPPRSSSTAWRS"
     gene            1863..2093
                     /locus_tag="PKI33_01865"
     CDS             1863..2093
                     /locus_tag="PKI33_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35774.1"
                     /translation="MTTTTQKRQIPWYLWPFAAIWKLLAVIVEMTGRLVAMILGIVLF
                     VVGVLVSLTIVGAIVGVPLGIVGLLLFFRGMF"
     gene            complement(2131..2811)
                     /locus_tag="PKI33_01870"
     CDS             complement(2131..2811)
                     /locus_tag="PKI33_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNM35775.1"
                     /translation="MKIFNRYTPLQIAIHLYAWSALVWIIAELFMGTFSINPIQELEQ
                     RTGRHAITLLVLSLLCTPLNTIFKFPELLKRRRTLGLYALLYATIHVLIFINLDYGLA
                     WSMIARTVFEKPYIIVGALAFLMLIPLGITSFDIWKVRLGKNWKRLHQMVYVIAPLVV
                     LHYLWSKKGDLLNFSGDIVRPTIYGLVVVIFLILRIPAIRRAIASLPNRGLTLPFKKN
                     PSPKADAS"
     gene            complement(2860..3807)
                     /gene="msrP"
                     /locus_tag="PKI33_01875"
     CDS             complement(2860..3807)
                     /gene="msrP"
                     /locus_tag="PKI33_01875"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNM35776.1"
                     /translation="MSHRFKSVPVRSSEITPKEVYLSRRDFIKAAGVVAGSMALAACA
                     PESSATPVAQFNGPVLNDELGDPTNTFEEITNYNNYYEFTTNKEAVAPMAKDFTTTPW
                     TIEVSGMVSKPKTFGLEDLLSMFPQEERIYRLRCVEAWSMVIPWNGFPLASLLKAVEP
                     TSNAKYVRFETIYRPEEMKGQSSPFYPWPYQEGLRLDEAMNDLTLLATGIYGETILPQ
                     NGAPIRLVVPWKYGFKSIKSIVKIELTDKEPTTLWSTIAPNEYGFYANVNPTVDHPRW
                     SQASERRIGELTRRETLMFNGYGEQVADLYADMDLNLNY"
     gene            complement(3891..4595)
                     /locus_tag="PKI33_01880"
     CDS             complement(3891..4595)
                     /locus_tag="PKI33_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNM35777.1"
                     /translation="MGYLLLEGGAEFGGRMSEPDLRAMELAGGFEAPIAILPTAAAPD
                     HNHKRAGNNGIRWFQSLGAKHVFSVDVIDAQSANDPKLADQLRNSKLIYLLGGFPRYL
                     GETLKGSACWSAAMEAYAKGAILAGSSAGAMVLCEHYYDPYEQKLLAGLNLVPNACVL
                     PHHNNFGKSWAKQLKQTLPDITLIGIDERTGILSTDTLGTEVQWGVYGGGEVTLYRSE
                     ATTGYERGASFQLKIS"
     gene            complement(4599..4988)
                     /locus_tag="PKI33_01885"
     CDS             complement(4599..4988)
                     /locus_tag="PKI33_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM35778.1"
                     /translation="MSDLLKITKTEGKVTILHLEGVLDGQTEKGFVDAAHAEFEAGKT
                     ALLLDFGKLDIITSAGLRALQNLYKKFTPEEEIQAWNAEHQGEVHKAKNLRVAQASSE
                     VTYILSITGFLQSLYVYADIQEALDSF"
     gene            complement(5022..>5764)
                     /locus_tag="PKI33_01890"
     CDS             complement(5022..>5764)
                     /locus_tag="PKI33_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35779.1"
                     /translation="LEVGSADAILIQTPEGRNVLINGGASASELSDELGRRLPFFTRK
                     LDWLIIAATDEDQLAALPRVVERYEPENVLWSGNVQASFSARTLDEYFAEKEIPVSLA
                     EAGQRLELGDGAFIEVQAAGPRGSVLLVEYGSFRALLPIGVSEGTFESLEFGNAIGKV
                     DVLLLADSGFAPSNPPDLLENLNPQLIVLDVAAGDPDGLPSQDVLDMLEGYSLLRTDR
                     NGWVDVRTDGTEMRIQVERGEDTVKSEP"
BASE COUNT         1419 a         1305 c         1536 g         1504 t
ORIGIN      
        1 ccctgcaatt tacaatgcag agacgatctt gcctgaccat gatcaactgg caaaaatgga
       61 acctgcataa cagaaaacaa aaaagaggag ttgagatacc tcaactcctc ttttttgttt
      121 atctaatctt ctgctgcgcg ggctacttct tcaggttcga agacaacttc ttctttgccg
      181 gtgagtttgg cttcgatctt tgagacaatt aagcgtaggt gaccgttatg ggcaacataa
      241 tctaggtcgt cggctgggac ggcgaggacg gggcagaggg tgaagttgtc gatccaacct
      301 tcgtagaggt cattcaggct ttgcagatag tcgggagcga tggtgcgttc atagtcacgc
      361 ccgcggtggt tgatgcggtt taggagggta tctacagagg cgcggaggta aataacgagg
      421 tctggtggcg gcaggaattg aacggcagtt tcgtacaggt cgcggtaggt ttgataatcg
      481 cggtctgtca tgttgccttg ttggtagaga ttgtgggcaa atatctcggc atcttcatac
      541 acgctgcggt cttggatgac ggaattggga tgttgagcca ggttgaggtg agagcggagg
      601 cggtgagtca agaagaagac ttgggaatga aatgaccaag aggacatgtc cttgtaaaaa
      661 tccgcgaggt atgggttttg ggtgacgggc tcatagaatg gatcccagcc catttggttg
      721 caaagcattt gtacgagcgt ggactttcct acaccgatat ttcccgcaat ggcgataaat
      781 cgtttcatgg cattctctct gagtggttta ttatttacat cttaccactg ggataaattt
      841 tggcacggag ttttaagaaa aaaggcggac gataccgtcc gccttttaag attgtatttc
      901 taaagcttag gggattttac ccgcgagttc catatcgata atttgggtga aggaagaaag
      961 cggctgcgcg cctacgatcg ccaagccgtt gacgaagaac gtgggggtgg attgaacgcc
     1021 aaggttagag cgaagtccat atcctgttgg atgttttcat catgcttgcc gctatcgaga
     1081 caggcagaga actcgtctac gtttaggttc aaatcggtgg catattgaat aaaggtatcg
     1141 cgcgttagag tttcgctgct gaagagttta tcgtgatatt cccaatacac attctggtca
     1201 tttgcgcaga gggaagcaat cgcggagggc atcgcatcgg gatggatgga ggtgagaggt
     1261 agattgcgat atacgaagcg gatctgaccg ggataagcat ccaacaatgc ctgatatgtt
     1321 tccgaatgga agcggcggca gaatggacac tggaagtcac tgaattctac gatggtgatc
     1381 ggcgcatcat ctggacccag gctggggtag ccctcggtgg ggatatcgta gcgggtgtat
     1441 tgtggctgtg gggtaacagg cgcttcggcg atttcgccgg aaggttgttg ggctgcagca
     1501 ttattggcag cagcggctgg gtctgcagcg acggtaacgg tgttactgcc gcgtccccaa
     1561 atgacgaagc caagcaagag accaacagca aaggcaagga cgactagcac agagtaaaaa
     1621 tgactgcgct tgaaggtgat gaaatcttca gagtctggcg tgctttcgat gacaggctct
     1681 tcttgttcga tttcgatcac ggcgggcgtg gattttttag accgggtaga ttttgtgttc
     1741 atagtggcaa gattatagtc ctgtgttttg gatttaagca gtagtactta agtatttttc
     1801 attctacttt ttagagaacc atttgtccta ttttgcgtat attaggcatg gaggttcttc
     1861 ttatgactac aacaactcag aaaagacaaa ttccttggta tttatggccc tttgccgcga
     1921 tctggaaatt gctggctgtc attgtagaga tgactgggcg gctcgtggcg atgatccttg
     1981 gcattgtgct gtttgtcgtt ggcgttctcg tcagcctgac aattgtcggt gcgattgtgg
     2041 gcgtgccact tggaatagtc ggtttactgc tgttctttcg cgggatgttt taaagagtaa
     2101 gggcgacctg tcaggtcgcc cttatttaaa ttaagaagca tccgctttgg gagagggatt
     2161 ctttttgaag gggagagtca agccgcggtt cgggagagag gcgatggcac gtcgaatggc
     2221 gggaatgcgc aaaatcagga agatcacaac gaccaagcca tagatcgtgg ggcgcacgat
     2281 gtcgcctgag aaattaagta ggtcgccttt tttgctccac aggtaatgca gcaccaccaa
     2341 gggcgcaatg acatatacca tttgatgaag gcgtttccaa tttttgccga ggcggacctt
     2401 ccaaatatcg aatgaagtta tgccaagggg aatgagcatg agaaatgcca acgcaccaac
     2461 gatgatatag ggcttttcaa aaacggtacg agcgatcatg ctccatgcta gaccataatc
     2521 gagattgata aagattagca catggatggt ggcatataac aaagcatata agcctaaggt
     2581 gcgacggcgt ttgagcagct caggaaattt gaatatcgtg ttgagcgggg tgcataataa
     2641 cgatagcacc aaaagagtaa tggcatgccg ccctgtgcgt tgttccaact cttgaatggg
     2701 gttgatcgag aacgtcccca tgaacagctc agcaatgatc cataccaggg cagaccaagc
     2761 gtaaaggtga atggcaattt gtaaaggggt atagcgatta aagattttca tgaccttagt
     2821 aaacgtcgga agtcttgagg cttccgacgt ttcatttctt tagtaattca agttcaagtc
     2881 catatcggcg tataagtctg cgacttgttc gccgtagccg ttgaacatca acgtttcgcg
     2941 gcgggtcagt tcgccaatgc ggcgttcaga cgcttgtgac caacgtggat ggtcgacagt
     3001 tgggttgacg ttggcataga agccatattc attgggcgca atcgtgctcc acaatgtggt
     3061 gggctctttg tcggtcaatt cgatcttgac gatggatttg atggatttga agccatactt
     3121 ccacggcacc accaaacgaa ttggcgcccc gttttgaggg agaatggttt caccgtaaat
     3181 accagttgcc agcaaggtca ggtcgttcat ggcttcatcg aggcgtaacc cttcttgata
     3241 gggccatggg tagaacgggc tgctttgtcc cttcatttct tcggggcgat aaatggtttc
     3301 aaagcgcaca tactttgcat tggacgtggg ttcaacggct ttcaacaaac tcgcgagcgg
     3361 aaacccattc catggaatga ccatgctcca ggcttctacg cagcgcaggc gataaatgcg
     3421 ctcttcctgt gggaacattg aaagtaagtc ttccaaacca aaagtcttcg gcttgcttac
     3481 cataccgctt acttctatgg tccacggcgt ggtggtgaag tcttttgcca tgggcgctac
     3541 ggcttctttg ttggtggtga actcgtagta attgttgtag ttggtaattt cttcaaaagt
     3601 gttggtgggg tcacccagct catcgttcag aacaggacca ttaaattgag ccactggggt
     3661 tgcagaggat tctggggcgc aagcggcaag tgccatactg ccagcaacca cacccgcagc
     3721 tttgataaag tcacgccgag agagatacac ctctttgggg gtgatctcag aagaacggac
     3781 aggtacagat ttaaaacgat gactcatgga ggtctctcct cgttcaatga tttactttaa
     3841 tggacacact gattctaaaa aaactttcta acggacagat gataatttgc ttaggaaatc
     3901 ttcagttgga agcttgcgcc tctttcgtag ccggtagttg cttcgcttcg gtaaagtgtc
     3961 acctcaccgc cgccgtagac tccccattgt acttctgttc cgagcgtatc tgttgacagt
     4021 atccccgtac gctcgtcaat tccgataagt gtgatatctg gcagcgtttg ctttaactgc
     4081 ttcgcccagc ttttcccaaa attattgtga tgggggagga cgcaagcatt tggaaccaga
     4141 ttcaaccctg ctaaaagttt ctgttcgtag gggtcgtaat aatgttcgca cagcaccatg
     4201 gctccggcac tgctcccggc aaggatcgcg cctttggcat aggcttccat ggcggcagac
     4261 caacaggcac tgccttttaa cgtctcgccg aggtaacggg gaaagccgcc gagcaggtaa
     4321 attaattttg aattccgaag ctggtcggcg agcttggggt cattggcaga ttgtgcatca
     4381 ataacatcca cagagaaaac gtgctttgcc cccaggcttt gaaaccagcg aatgccattg
     4441 ttccccgctc gtttgtgatt gtggtctggc gcggcggcag tgggcagaat agcgatcggc
     4501 gcttcgaacc cacctgccaa ttccatggcg cgcagatccg gctcagacat tcgcccgcca
     4561 aactccgcgc cgccttccag taaaagatag cccatcgcct aaaaagaatc caatgcttct
     4621 tgaatatcag catagacata caagctttgt aaaaagccag taatgctgag aatgtacgtc
     4681 acttctgaag aagcctgcgc aaccctgaga tttttggctt tatgaacttc gccttgatgt
     4741 tcagcattcc atgcttgaat ttcttcttca ggagtaaatt ttttataaag attttgcaac
     4801 gcacgtaacc cggcgctggt gatgatatcc agcttgccaa aatcgagtag cagggctgtt
     4861 ttcccagcct caaactcagc atgcgccgca tccacaaacc ccttttcagt ttgtccgtct
     4921 aatacccctt ctaaatgcaa tatggtgact ttcccttcgg tctttgtgat cttcaataaa
     4981 tcagacatct ctctctcctt ggttttgatg tacgctaaaa attacggttc gctttttaca
     5041 gtgtcttcgc ctcgttctac ttgaatacgc atttctgtcc catcagtgcg gacgtcaacc
     5101 cacccgttgc ggtccgtacg aagcagagaa tagccttcca acatgtctaa tacatcttgt
     5161 gaaggtaaac catcggggtc accagccgca acatccaaaa caatcagttg cggatttaaa
     5221 ttttccaata ggtcaggcgg gttgctggga gcaaagcctg aatcggcgag taaaagcaca
     5281 tccactttgc cgatggcatt gccaaattca agcgactcga atgtcccttc gctgacgccg
     5341 atgggcaaca aggcgcggaa actgccatac tcgacgagta acacgcttcc acgcggaccc
     5401 gctgcctgca cctcgataaa cgcgccatca cccagctcaa gccgttgacc tgcttctgcc
     5461 aggctgacgg ggatttcctt ctctgcaaag tattcatcca aggtgcgggc agagaatgaa
     5521 gcttggacgt tcccactcca tagcacgttc tctggctcgt atcgttctac aacgcggggg
     5581 agggcagcca attggtcttc atctgtagcg gcaataatga gccaatctaa tttgcgggtg
     5641 aaaaagggca aacggcgacc gagttcatct gaaagttcag aggcgctggc accgccattg
     5701 ataaggacgt ttcgtccctc aggggtttga atgaggatag catctgcgga accaacttcg
     5761 agaa
//
LOCUS       DAOREF010000057         5718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7625_length_5718_cov_4.422921, whole genome shotgun sequence.
ACCESSION   DAOREF010000057 DAOREF010000000
VERSION     DAOREF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7625_length_5718_cov_4.422921"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKI33_01895"
     CDS             complement(<1..234)
                     /locus_tag="PKI33_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35780.1"
                     /translation="MNLQTIIEKLQLNILTEPRDFSSITPKGGYTSDLLSCVMAGAKN
                     GNIWVTLQAHMNIVAVAAMLDVSAVIITENAQPD"
     gene            complement(237..1238)
                     /locus_tag="PKI33_01900"
     CDS             complement(237..1238)
                     /locus_tag="PKI33_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNM35781.1"
                     /translation="MDPTRQPTEQEKFGRHRPLTDRDAHEINRIEELSYDLKVREVMI
                     KNVITTTSDVPLSSALELLRVNRISGLPVVSDGTLIGVISLEDIVRALQTNGLSLPVK
                     AYMSTTLVTVKTTDSVVEAIKTFSEKRLGRLPVVDEHQKLEGIITKGDITRGILLAVE
                     RDYKEEEVRRYRASHLFDDIVSDRTTLVLRYNIKVGDFMHGGNASSNIKRALLRLGAD
                     AQLARRCGIAAYEAEMNLIIHTTNGGVLKLEVEPHRISLITTDDGPGIPDVQKAQQAG
                     YSTATDKVREMGFGAGMGLVNIKRCVDSMELESTTGKGTKLTMRINVPTESLNSKKG"
     gene            complement(1225..1578)
                     /locus_tag="PKI33_01905"
     CDS             complement(1225..1578)
                     /locus_tag="PKI33_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35782.1"
                     /translation="MKDIIVIAEGELLTPELNTDVNIHGGCCADLMSDVLAYSRPRAV
                     LLTGLTNPQVVRTAQMADFRAIVFIRGKRPQQETIDIANQENIPLITSPMGMFELCGK
                     LHDSGLKSYEGNGSD"
     gene            complement(1770..2048)
                     /locus_tag="PKI33_01910"
     CDS             complement(1770..2048)
                     /locus_tag="PKI33_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35783.1"
                     /translation="MSEERAKPKAFATLCGRIFRDSDPQYFPFAEYKGKKILLCTEAC
                     LGAFLADPDIFCKVHRNSDKTAARMKKEIEHLATLWSQYASERPTEEK"
     gene            complement(2045..3763)
                     /locus_tag="PKI33_01915"
     CDS             complement(2045..3763)
                     /locus_tag="PKI33_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNM35784.1"
                     /translation="MTSLKWWQTAVFYQIYPRSFADGNGDGIGDFKGITEKLDYLVSL
                     GVDALWLSPHFPSPNWDCGYDISDYTNVAPEYGTLEDFRTFLKESHKRNIRVILDLVL
                     NHTSDEHPWFLESKSSRDNPKADWYVWVDTPPNNWQSCFDGDAWTYVPERKQYYYHYF
                     MKQQPDLNWHNPEVKAAMWDAVRFWLDLGVDGYRLDAIGTIYEDANLTPHTVPMTLAE
                     LRRFSELAETEEDKKLREKYWVEMFKNQWGQPGVHELMKELRSILNEYDGDRMLVGED
                     DNIDFMGDGEDELHLVFNFPLANVNRITPEHIRKNQTERIARLNQLAKGGWACNTLGN
                     HDRSRIYTFYGDKLHDAELARLNAALTLTLRGTPFLYNGEEIGMTDYLLPHISQVKDT
                     MGSWYYHTIVTEMGVDPEEAMFRTAEMTRDKNRTPMHWSNKANAGFSPEGVETWLPIN
                     PNYKEGINVFEQEQNPSSILNYYKHLLKVRKASPALVEGDYLPLHEDATDYFAFIRKS
                     DKQTVLVVLNYSDKRLRLNFSSDQDVNGKNLHTLFTSAVRSPADHKADNLTIAPFEVF
                     IAEVTA"
     gene            complement(3816..4082)
                     /locus_tag="PKI33_01920"
     CDS             complement(3816..4082)
                     /locus_tag="PKI33_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNM35785.1"
                     /translation="MIPDRVITLAELKRNTGERGTRMWIAHLGIVYDVTECPKWRAGM
                     HEFLHFPGQDLSSEMEDAPHEDEVFKHDCVKIIGKLEEQDVSTS"
     gene            complement(4079..4810)
                     /locus_tag="PKI33_01925"
     CDS             complement(4079..4810)
                     /locus_tag="PKI33_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35786.1"
                     /translation="MTNQTPIINFSPKKILIFFFSLVALLVALSIWGQHMRFFGVGDI
                     RGPIHEMFIDIMMTSFYLDYESNVPTFINALMLFIPALLLLAIGLWKSNIKDKYRFQW
                     NALAFIFFLLSIDEIASFHERLIKPMRAAVGSHGVFFFAWIIPGMAAIALFGFAFLTF
                     FLHLDKKFKLLFFFSLAVYISGVIGGEMVSGYFAANLGQKNFTYAVVASLEESVEMFG
                     CGLIIYSLLEYIKHYLPEGLTFKGS"
     gene            complement(4861..>5718)
                     /locus_tag="PKI33_01930"
     CDS             complement(4861..>5718)
                     /locus_tag="PKI33_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNM35787.1"
                     /translation="EVAGVVEKIGVSVTNVKIGDRVALHYNLSCGDCYQCSTGNDQFC
                     EKVLMLGHYTNGGYAEYIAVPARNAVHLPEEIPFEQGATLMCASATAFHALRKSRIQG
                     GETVAIFGAGGLGQSAIQLAKAFGALEVYAVDINESKLDLAKQHGAIPVNAQKVDAVA
                     EIKRLTHNKGVNVAIEMIGLPQTMKQALQVAGVMGRVVIVGVSNKMLEVQTYTELLGN
                     EVELIGSNDHHLQELPLLVDLARRNVLDTSKAVSKIIPLDADAINAALDELEHFGSDV
                     RTVIVPASS"
BASE COUNT         1475 a         1283 c         1358 g         1602 t
ORIGIN      
        1 atcaggctga gcgttttcgg tgatgatgac ggctgagaca tcaagcattg ccgcaacagc
       61 gacgatgttc atatgtgcct gtaatgtgac ccagatgttg ccattctttg caccagccat
      121 aacgcatgaa agcaagtcgg acgtgtagcc acccttggga gtaatggatg aaaaatcacg
      181 cggttcggta aggatattga gttgaagttt ttcgatgata gtttgaagat tcatagttat
      241 ccctttttgc tgtttaggct ttcagttggg acgttaatgc gcatggtgag ctttgtgcct
      301 ttaccagttg ttgattcaag ctccattgag tctacacagc gttttatgtt gaccaacccc
      361 atacctgcgc caaagcccat ttcgcgaact ttatctgtcg ccgtggagta acctgcttgt
      421 tgtgcttttt gcacatcggg aatgccagga ccatcatcag tagtgattag gctaatgcga
      481 tgaggttcca cttcaagttt gagaacgccg ccattggtgg tgtggataat tagattcatc
      541 tctgcttcat aagcagcgat tccgcagcgc cgtgccagtt gtgcatcagc gccgaggcga
      601 agcagggcac gtttgatatt gctggaggca ttaccgccat gcataaaatc tccaactttg
      661 atgttgtatc gtaaaacaag ggtagtgcgg tctgaaacaa tatcgtcgaa taaatggctg
      721 gcgcgatatc gccgaacttc ttcttccttg taatcacgtt ctactgccaa tagaataccg
      781 cgtgtgatat cccctttggt gatgatgccc tctaactttt ggtgttcatc caccaccggc
      841 aaacgcccca gccgtttttc gctgaaggtt tttatcgctt caacaacact gtctgtagtt
      901 ttgacggtga caagggttgt gctcatgtag gctttaactg gaagcgataa tccgttggtt
      961 tgcagagctc gaacaatatc ttccagactg atcacaccaa tcaaggtgcc atcgctgacg
     1021 accggcaagc cagagatacg gttgactctc aaaagctcca atgcactgga aagcggcaca
     1081 tcagatgtgg ttgtgatgac gttcttgatc atcacttcgc gcactttcag gtcatatgaa
     1141 agttcctcga tgcgattgat ctcatgtgca tcgcggtctg tgagcggacg atgccgccca
     1201 aacttttctt gttcagtcgg ttgcctagtc ggatccattg ccttcgtagc tttttaatcc
     1261 tgagtcatga agttttccgc atagctcaaa catacccatt gggcttgtga tcaacggaat
     1321 gttttcttga tttgcgatat cgattgtctc ttgctgaggt ctcttgccgc ggataaaaac
     1381 gatcgcgcgg aaatctgcca tttgcgcggt tcggactacc tgtgggttgg tcagccccgt
     1441 cagcaatacg gctcgcggac ggctgtacgc cagtacatcg ctcattaagt ctgcacagca
     1501 accgccatga atgtttacat cagtattcag ctcgggggtt agtaattcac cttctgcaat
     1561 cacaataatg tctttcaaat tcatattcat atataaaggg atggtccttt cacctcataa
     1621 taaaactctc tttaaaatta ttccctactt agtctgttta ggtaagtttc ttttctcatg
     1681 ctcagtaggt gactattgac ctgttaagtg ataaatttca caaatatata acaaataaaa
     1741 aaacgctcta aagagcgttt ttttatttgt tacttttctt cagtaggtct ttcagaagcg
     1801 tattggctcc ataaagtagc tagatgttcg atttcttttt tcatgcgagc tgcagtttta
     1861 tctgagttgc ggtgaacttt acagaaaata tctgggtctg cgagaaaggc gccaaggcaa
     1921 gcctcggtgc aaagcaaaat tttcttgcct ttgtattcag caaaaggaaa atactgtggg
     1981 tcactatctc ggaagatacg tccgcacagg gtggcgaatg cctttggttt cgcccgttct
     2041 tcactcatgc agtgacttcc gcaatgaaga cttcaaatgg cgcaatggtg aggttatctg
     2101 ctttatggtc agcaggggag cggacagcac tggtgaacaa ggtatgtaag ttctttccat
     2161 tcacatcttg atcacttgag aaattcaagc gtaagcgttt atcggaataa ttcaatacaa
     2221 ccaataccgt ctgtttgtct gactttctaa tgaatgcaaa gtagtccgtt gcatcttcat
     2281 gaagcggaag ataatctcct tctaccaatg caggtgacgc cttacggacc ttgagcaagt
     2341 gcttgtagta attcaagatc gagcttgggt tttgctcctg ctcaaaaaca ttgatgccct
     2401 ctttataatt tgggttgatc ggcagccaag tttctacacc ttctggagaa aaaccagcat
     2461 ttgctttatt tgaccaatgc atgggggtgc ggttcttgtc gcgggtcatt tctgctgtgc
     2521 ggaacatggc ttcttctggg tccacgccca tttcggtgac gatggtatgg taataccagg
     2581 agcccattgt gtctttaacc tgcgagatat gcggcagaag atagtcggtc atgccgattt
     2641 cttcaccatt atagaggaaa ggggtgccgc gtaaagtaag ggtaagggca gcattaaggc
     2701 gagctaattc ggcgtcgtgc agcttatcgc cataaaacgt gtaaatgcga gaacggtcgt
     2761 ggttacccaa tgtattgcaa gcccagccac ctttggcaag ttgatttagg cgtgcaatcc
     2821 gttcggtttg attttttcga atgtgttccg gagtaatgcg gtttacattt gccaagggga
     2881 agttaaagac aaggtgcaac tcatcttcgc cgtcgcccat aaaatcaatg ttgtcatctt
     2941 cgccaaccag cattcggtcg ccgtcatatt cgttgagaat gctacgcagt tctttcatca
     3001 actcgtgaac gccgggttga ccccattgat tcttgaacat ctcgacccaa tatttttctc
     3061 taagtttctt atcttcctcg gtttctgcaa gctctgagaa gcggcgcaat tcagcaaggg
     3121 tcataggaac ggtatgaggc gttaggttgg catcttcata aatggtgccg attgcgtcaa
     3181 gacgataccc atccacacca agatccagcc aaaagcggac cgcgtcccac atagcggctt
     3241 tgacttctgg attgtgccag ttcaaatctg gctgttgttt cataaagtag tgatagtaat
     3301 actgttttcg ttcgggaaca taggtccatg catcgccatc gaagcaggat tgccagttgt
     3361 tgggcggagt atctacccaa acgtaccaat ccgctttggg gttgtcgcgg ctggacttgg
     3421 attccaggaa ccaagggtgt tcgtcagaag tgtgattcaa gacaaggtcc agaatgacgc
     3481 ggatgtttcg cttgtgagac tcttttagga aggtcctgaa atcttcgagg gttccatatt
     3541 ctggggcaac attggtgtaa tctgaaatgt cgtaaccgca atcccaattg ggggagggaa
     3601 aatgcggcga aagccaaagc gcatccacac cgagactgac gaggtaatca agtttttcgg
     3661 taataccttt aaagtcaccg atgccatctc cattgccatc agcaaacgaa cgcggataga
     3721 tttgataaaa aacagcagtt tgccaccatt tgagtgatgt cattgtagat gtaattcctt
     3781 ttgcattgag agtttgaaag tttataggca agaaattaag aggttgaaac gtcctgctct
     3841 tccaacttcc caattatttt tacgcagtca tgcttgaata cttcatcttc atggggtgca
     3901 tcttccattt cgctgctcag atcctgacct ggaaaatgca gaaactcatg cattccggct
     3961 ctccacttgg ggcattcggt cacatcgtag acgatgccaa gatgggcgat ccacatgcgc
     4021 gtcccacgtt cgcctgtatt gcgttttagc tctgcgagag tgataacacg gtctggaatc
     4081 atgagccttt gaacgtgagc ccttcgggaa gatagtgctt gatgtattca agcagggaat
     4141 aaatgatgag tccacagccg aacatttcaa cagattcttc taaactggcg accacagcgt
     4201 aggtgaaatt tttctgcccg aggttggcag caaaatatcc actaaccatt tctccgccga
     4261 ttacgccgct aatatatact gccagcgaga agaaaaagag caatttgaac tttttatcga
     4321 ggtgtaagaa aaaggtgaga aaagcaaagc cgaaaagtgc aatagcagcc atgccgggaa
     4381 taatccaagc aaaaaagaaa acgccgtgcg aaccgactgc agcacgcatc ggtttgataa
     4441 ggcgttcatg aaatgaagca atttcatcta tcgaaagcaa gaaaaagatg aaagcaagag
     4501 cattccattg aaaacgatat ttatctttga tgttgctttt ccacaagcca atggcaagaa
     4561 gtagaagtgc tggaatgaaa agcattaggg cattaatgaa ggtcggcaca ttgctttcat
     4621 agtccagata aaaggatgtc atcatgatgt cgatgaacat ttcgtgtatg ggaccgcgaa
     4681 tatcgccaac gccaaagaag cgcatgtgtt gtccccaaat gctgagtgcc actaacaacg
     4741 ctacaagaga gaaaaagaaa atcagaattt tcttggggct gaaattgatg ataggagttt
     4801 ggtttgtcat agatacctgt gagcgttgat tatacgtcga aacgctggac agttgtcggc
     4861 ttaggaagag gcgggaacaa tgactgtgcg cacgtcactg ccaaagtgtt ccaactcatc
     4921 gagcgcggcg ttaatggcat ccgcatctaa cggaatgatc ttgctgactg cttttgatgt
     4981 gtctaataca tttcgacggg caaggtcgac aagcaaggga agttcttgca agtgatggtc
     5041 gttggacccg atgagttcca cttcattacc aagcagttct gtgtaggttt gcacttccag
     5101 cattttgttc gaaaccccca caatgaccac gcgtcccatt acccctgcaa cttggagcgc
     5161 ctgtttcatg gtctgtggaa gtccaatcat ctcaatggca acattgacac ctttgttatg
     5221 agtaaggcgt ttgatctctg ccactgcatc tactttttgg gcattgacgg gaatcgcgcc
     5281 atgttgttta gcaaggtcaa gtttcgactc attgatatcg acggcatata cttcgagagc
     5341 gccaaacgct tttgccaatt gaatagcaga ctgaccgagt ccgcctgcgc cgaagattgc
     5401 taccgtttcc ccgccttgaa tgcggctctt gcggagtgca tgaaaggcag tggcactggc
     5461 acacatcaaa gttgcacctt gttcaaaagg aatttcttct ggtaagtgaa cggcattgcg
     5521 tgctggcacg gcaatatatt ctgcgtaacc gccgttggtg taatgcccaa gcatcaaaac
     5581 tttttcgcaa aattggtcat tgcctgttga gcattgataa caatcgccgc aggaaagatt
     5641 atagtgaagc gctacgcgat ccccaatttt tacattggtt acgcttacgc caatcttctc
     5701 aaccacacct gccacttc
//
LOCUS       DAOREF010000058         5661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7759_length_5661_cov_5.237424, whole genome shotgun sequence.
ACCESSION   DAOREF010000058 DAOREF010000000
VERSION     DAOREF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7759_length_5661_cov_5.237424"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1113)
                     /locus_tag="PKI33_01935"
     CDS             complement(154..1113)
                     /locus_tag="PKI33_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35788.1"
                     /translation="MTANSTAVATLDPEAITSKSTGMWQLTMRRLLRRRSAMAGLVIL
                     GILIFIAVFAPALAPYDPTISMLDADPPQRIKARVAPCIHLLGCPADQPQHLAGIDGN
                     IRDQFSRLLYGARLSLMVGFSTVTFAIIIGTILGAVAGYFGGWVDNSIMRIMDVLLAF
                     PSLLLAIAIVTVLGPGLINALLAIGIVSIPAYARVMRASVLSVREMEFVSATRALGGN
                     DMQILFLRILPNALTPLIVQGTLGIASAILDAAALSFLGLGAQPPTPEWGSMLGAERN
                     QVFTAPHLVFYPGFMIMLTVLAFNLIGDGLRDALDPRLAHSSG"
     gene            complement(1125..2207)
                     /locus_tag="PKI33_01940"
     CDS             complement(1125..2207)
                     /locus_tag="PKI33_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35789.1"
                     /translation="MMIQYILRRVIASIPVLFGILLVTFVLARVIPGDPCRAILGEKA
                     TEEVCSRFIEEKGLNKPIVTQFAIYVKDMASGNLGDSFRLGLPVSRLLIERLPTTFEL
                     SLAALIVSILFGIPLGIISAVKHNTWLDVVTMVWANMGVSMPVFWLGLMLMYVFSLML
                     KDTPFWLPPSGRVSPGIPDTPFYELWGWVVPETGLWSAFLNFTGRLNILNSLLTQNWE
                     LLKDSIKHLILPALALGTIPMAIIARMARSAMLDVLGQDYIRTAKAKGLGRGGVIFKH
                     AFRNSLLPLVTVIGLSFGGLLGGAVLTETIFGLSGVGRTLYEAITARDYGIVQVFTLV
                     IAFVFVVINLIVDISYAYLDPRVRLN"
     gene            complement(2291..4063)
                     /locus_tag="PKI33_01945"
     CDS             complement(2291..4063)
                     /locus_tag="PKI33_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM35790.1"
                     /translation="MKRNLFSLLSLLVLASLVLAACGSAEATTAPEATEAATEVAATE
                     AATEAPAAEATPTPIPDVSIATFDGTSLAVPAEECNGDYIGLIQSVVAVDASTVVFTL
                     CRPDPAFLSKIAFSPFAIYPSEWLEATAGDTTRTSEGLEKPVGTGPYMVSEWARGDSV
                     TFVANPDYWGEQPAASTLVMRWSAESAARLLELQSGTVDGIDNLGPDDFAVVEADSSL
                     QVAKRPALNTFYIGMTNTFAPFDNQKVRQAIAMGIDRQRIVDTFYPAGSEVATHFTPC
                     AIPNGCAGDAWYDFNVDAAKALLAEAGFADGFSTKLYYRDVVRGYLPQVGNVAQDIQA
                     QLKENLNIDAEIVVMESGAFIEASSAGTLDGLYLLGWGADYPHITNFLDYHFGASNPQ
                     FGTQSDTYTDLLVQGAQIADPAEAEPIYAQANNAIKEYVPMIPVAHGGSGTAYRADVT
                     NPQASPLTNEQFAFSAPGDRDTFVWMQNAEPISMFCADESDGESLRACDQVMEALYGY
                     EINGTATEPILAESCDPSEDLTVWTCTLRQGVKFHDGSDFDANDVVATFNMGLNIDSP
                     THVGNSNIWSYYDYLWGLMKKPGS"
     gene            complement(4135..5247)
                     /gene="amrS"
                     /locus_tag="PKI33_01950"
     CDS             complement(4135..5247)
                     /gene="amrS"
                     /locus_tag="PKI33_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNM35791.1"
                     /translation="MNALAKRLDALTVEGELYEKLADDSVRCFACGHRCLIREGKRGI
                     CQVRFNTGGRLMVPHGYVAALQSDPIEKKPFSHVLPGSNALTFGMLGCDYHCPYCQNW
                     LTSQAMRDPASDVSAQFIRELTPEDVLSYARRTNASAVISSYNEPLITSEWAVEIFKV
                     AKENGLMCAYVSNGNNTPEAMDYLVPYLSAYKVDLKCMSDKNYRKLGGTLQHTLDGIK
                     RARDHGLWVEVVTLVIPGFNDSNEELWDAARFLAEVSKDIPWHVTAFHKDYKMTEPAN
                     TDARTLIRAAEIGREAGLKFVYAGNLPGTVGEYEDTACPRCSYRLIKRRGYVIKEYHL
                     TDEGKCPKCGESMPGLWHKDPSRVGLNGSGFPMALQ"
     gene            5266..>5661
                     /locus_tag="PKI33_01955"
     CDS             5266..>5661
                     /locus_tag="PKI33_01955"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNM35792.1"
                     /translation="MLSVVIQAGGQSSRMGEDKALKPFLGKPLIQRVIDRLSPIADEM
                     IVTTNRPADYAFLNQRLIADLKPGRGSLGGLYTAIASARHPFVAVVACDMPFASKSFF
                     ESAHRLLVKEGADVLIAKTEAGYEPFHALY"
BASE COUNT         1504 a         1454 c         1506 g         1197 t
ORIGIN      
        1 ggacgacgaa cgagaaagtc gagagatcat cactggcgca ttgcttgttg cgctcagctt
       61 cggcgggctg accgtttggg cattcattca acacgctgca taacaaataa taaaaaagcc
      121 cgtcgtaaag acgggctttt ttgattcaat gttttatccg ctagagtgtg caaggcgtgg
      181 gtcaagcgca tcacgcagac catcaccgat caagttgaat gccaacaccg taagcataat
      241 cataaagccg gggtaaaaca cgaggtgtgg cgcggtaaag acttgattgc gttctgcacc
      301 gagcatggaa ccccattcag gcgtaggagg ttgagcgccc agtcccaaga aagacaaagc
      361 cgctgcatcc aaaatagcag aggcgatacc caacgtccct tgtacgatga gcggtgtcaa
      421 ggcattaggc aaaatacgca agaaaaggat ttgcatatcg ttaccgccca aagcacgagt
      481 agcagagaca aattccattt cacgaacaga gagcaccgaa gcacgcatca cacgtgcata
      541 agcagggatg gatacgatac caattgccaa caaggcgttg ataagacccg gtcccaagac
      601 ggtgacgatg gcaatggcaa gcaacagaga agggaatgcc aatagcacat ccatgatgcg
      661 cataatacta ttatcgaccc agccgccaaa atacccagct actgcaccaa ggatggttcc
      721 gatgatgata gcgaaggtca cggtggaaaa cccgaccatc aagctcaaac gtgcaccata
      781 cagcaagcgg ctaaattggt cacggatgtt tccatcaata ccagcaagat gttggggttg
      841 atctgccgga caccccaaga ggtgaataca aggcgctacg cgagctttga tccgctgcgg
      901 cgggtcggca tccaacatgg agatggtggg gtcgtatgga gcaagagcgg gggcaaaaac
      961 cgcaataaaa atcaaaatac caagaatcac caaacctgcc atggcagagc gccggcgtaa
     1021 cagtcggcgc atggtgagtt gccacatccc tgtggacttt gatgtaatcg cttcaggatc
     1081 cagtgttgct acagcggttg agttagcggt cattttcgag ttccttagtt gaggcgaaca
     1141 cgaggatcaa gataggcata agaaatatcc acgatcaggt tgatcacaac gaagacgaat
     1201 gcgatcacca gagtaaagac ctgcacaata ccataatcac gagcagtgat ggcttcgtaa
     1261 agggtgcgcc ctacaccaga aagaccgaag atcgtttcgg tgagaaccgc tccgcccaag
     1321 agaccgccaa aggacaaacc gatcacggtc accaacggca ggagggaatt gcggaaggcg
     1381 tgtttgaaaa ttacgccgcc tcttccaagt cctttcgcct ttgccgtgcg gatgtaatcc
     1441 tgtcctaaca cgtcgagcat ggcagagcgc gccatacgag cgatgattgc catcggaatg
     1501 gttccgagcg ccaacgccgg caaaatgaga tgttttatcg agtctttcaa caattcccaa
     1561 ttttgagtca gcagagaatt gaggatgttc aaacgacccg taaagtttaa gaaagcagac
     1621 caaagtcctg tctctggtac aacccagccc cataattcat aaaatggggt atcagggata
     1681 ccagggctaa cacgcccgga aggcggcagc cagaaaggtg tatctttcaa catcaaagag
     1741 aacacataca tcagcattaa cccaagccag aagaccggca tggaaacgcc catgttcgcc
     1801 cacaccatgg tgactacatc taaccaggtg ttgtgcttta cggcagaaat aatgcccaag
     1861 ggaatgccaa agaggatgct aacgatcagg gcagccagac tcagctcaaa ggtcgtaggg
     1921 aggcgttcga tcaacaaacg cgaaacaggc aacccaaggc ggaaggagtc tccaaggttg
     1981 ccgcttgcca tatccttaac atagatcgca aattgtgtga cgatgggctt attgagccct
     2041 ttttcttcaa taaatcgaga acagacttcc tctgttgctt tttcccccaa aattgcccgg
     2101 caagggtcgc caggaataac gcgtgcaagc acaaaagtaa caagcaaaat gccaaataat
     2161 accggaatgg atgcgatcac acggcgaagg atgtattgga tcatcatgat gggcaaggct
     2221 ccctgagaat acgggatggc gggttacgcc atcccgtatt tttttcatac aaacttggtt
     2281 ccgaaacgaa ttaggaaccg ggcttcttca tcaagcccca gaggtagtcg tagtagctcc
     2341 agatgttgct gttaccaaca tgggtcgggg agtcgatgtt caagcccatg ttgaaggtgg
     2401 caaccacatc attggcatcg aaatcgctgc catcgtggaa cttcacgccc tggcggagag
     2461 tacaagtcca gacggtgagg tcttcgctcg gatcgcagga ttcggcgagg atgggttcag
     2521 tggcggtgcc attgatttcg tagccgtaga gggcttccat cacctgatcg caagcgcgga
     2581 gggattcgcc atcggattcg tcagcacaga acatgctgat gggttcggcg ttctgcatcc
     2641 aaacgaaggt gtcgcggtcg ccaggagcgg agaaggcgaa ctgttcgttg gtgagcgggc
     2701 tagcctgcgg gttggtcacg tcagcgcggt aagcggtgcc agaaccaccg tgagcaacag
     2761 ggatcatcgg aacgtattct ttgatcgcgt tgttggcctg agcgtagatc ggctcagctt
     2821 cggcggggtc agcaatctga gcgccctgaa ccaagaggtc cgtgtaggtg tcagactgag
     2881 taccgaactg cgggttggaa gcaccgaagt ggtagtccaa gaagttggtg atgtgcgggt
     2941 aatcagcacc ccagccgagg aggtacaaac catcgagggt accagcggag gaggcttcaa
     3001 tgaaagcacc ggattccatc acaacgattt cggcatcgat gttgaggttt tccttcaact
     3061 gagcctggat gtcctgagcc acattgccaa cctggggcaa gtaaccgcgg actacgtcac
     3121 gatagtacaa cttggtggag aagccatcgg cgaagccagc ttcggcgaga agagccttgg
     3181 cggcatcgac attgaagtca taccaagcat caccagcaca accattcggg atggcgcagg
     3241 gggtgaagtg ggtggcaact tcggagccgg cggggtagaa ggtatctaca atgcgctggc
     3301 gatcaatacc catggcgatc gcctgacgaa ccttctggtt gtcaaacgga gcgaaggtat
     3361 tggtcatacc gatgtagaag gtgttgaggg cggggcgctt cgcaacctgg aggctggaat
     3421 ccgcttcgac cacagcgaaa tcatcaggac cgaggttatc gataccgtcc acggtgccag
     3481 actgcagttc gagcaagcga gcagcagatt cagcagacca gcgcatcacg agagtggagg
     3541 cggcaggctg ttcaccccag taatcggggt tggcaacgaa ggtcacgcta tcaccgcgag
     3601 cccattcgct caccatgtaa ggaccagtac cgacaggctt ttcgagacct tcgctggtgc
     3661 gggtggtgtc gccagcggtg gcttcaagcc attcggaagg atagatcgca aacgggctga
     3721 acgcgatctt ggagaggaag gcgggatctg ggcggcagag tgtgaagacc acggttgagg
     3781 cgtcaaccgc aaccacagac tggatcaaac caatgtaatc gccattgcat tcttcagcag
     3841 gcacagcaag ggaagtgcca tcaaaggtag cgatggaaac atcggggatc ggggtggggg
     3901 tggcttcagc agcaggtgct tctgtcgctg cttcggtggc tgcaacttca gtcgcagctt
     3961 ccgtcgcttc tggtgcagtt gtggcttcag cgctaccaca agccgcgaga acaaggctcg
     4021 cgagcaccaa taacgaaagc aaagagaaca gatttcgttt catacaggtt tctcctccat
     4081 gtgagaaagg ggatttattc attttgccgc gcaaaatgtt gcggaattat acacctattg
     4141 caaagccatc ggaaatccac ttccatttaa ccctactcgc gaagggtctt tatgccataa
     4201 accaggcatg gattcgccac atttcgggca ttttccctcg tctgtaaggt gatattcctt
     4261 gatgacatat ccccgcctct ttatcagccg gtaactgcat cgtggacacg cagtatcttc
     4321 atactccccc accgttcccg gcaagttacc cgcatagacg aacttcagcc ccgcctcacg
     4381 cccaatctcc gccgcacgga tgagcgtccg ggcatcggtg ttggctggct cggtcatctt
     4441 gtaatcttta tggaaagccg tcacatgcca aggaatatct tttgaaacct cagccaggaa
     4501 ccgtgctgca tcccacaact cttcatttga gtcattaaag cccggaatga ctaaggtcac
     4561 cacttctacc cataaaccgt ggtctctggc acgcttgatt ccatctaaag tatgctgcaa
     4621 ggtgccaccg agtttgcgat aattcttatc gctcatgcac ttcaggtcaa ctttataggc
     4681 agaaagatat ggcaccagat aatccatcgc ttcgggcgtg ttattaccat tcgagacata
     4741 cgcgcacatc aacccatttt ctttagcaac tttgaaaatc tctactgccc attcactggt
     4801 gataagcggc tcattatagg aagaaataac tgccgaagca ttcgtccgcc gcgcatagct
     4861 cagcacatct tctggagtca actcgcgaat gaattgagca gaaacatctg aagccgggtc
     4921 tcgcatggct tgggaagtga gccagttctg gcagtatggg cagtgatagt cgcaaccaag
     4981 cataccgaag gtcaaggcat tggagccggg cagtacatgc gaaaaaggct tcttttcaat
     5041 tgggtcactt tgcaatgccg ccacataccc atgcggcacc attaacctgc cacctgtgtt
     5101 gaagcgtacc tgacaaattc cccgcttgcc ttcgcggatc aggcagcgat gtccgcatgc
     5161 gaaacaacgc acagaatcat cggcaagttt ttcgtacaac tcaccttcta cggtcagagc
     5221 atcaagccgt tttgccagag cattcatatc acctataata tactcatgtt atcggttgta
     5281 atacaagcag gcgggcagtc ttctcggatg ggcgaagata aggcgctcaa gccttttctt
     5341 ggcaagccat tgatccaacg tgtgatcgat aggctctctc ccattgccga tgaaatgatt
     5401 gtgacgacca atcgtcccgc agactatgcc ttcttaaatc agcgcctcat cgcggacctc
     5461 aaaccgggtc gcggctcctt aggcggactc tacactgcca tcgcatctgc ccgccatcca
     5521 ttcgtggcgg tggtggcatg cgatatgcct ttcgccagta aaagcttctt tgaaagcgca
     5581 cacaggctcc tcgttaagga aggggcagat gttctcatcg caaaaacaga agcaggctat
     5641 gaaccgttcc atgctttata t
//
LOCUS       DAOREF010000059         5624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_7855_length_5624_cov_3.779134, whole genome shotgun sequence.
ACCESSION   DAOREF010000059 DAOREF010000000
VERSION     DAOREF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7855_length_5624_cov_3.779134"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKI33_01960"
     CDS             <1..427
                     /locus_tag="PKI33_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35793.1"
                     /translation="LGGHGDEMVPLTRHSNIAGVPLKEYFPADKLEAIVNRTRKGGGE
                     IVNLLKTGSAYYAPSMACVQMAEAILKDKKLIVPCATLMQGEYGLNDMYFGVPVMLGA
                     GGMEKVIEYKFDADEKAMFEKSAASVRESHDALKSVVSL"
     gene            499..1818
                     /locus_tag="PKI33_01965"
     CDS             499..1818
                     /locus_tag="PKI33_01965"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HNM35794.1"
                     /translation="MILHEKMSAQLPAWRERIKSLAKEHGEVVVDKVTVGQIVGGMRD
                     IKSLLTDVSFVDPAEGIRFRGMSIPEVLKALPKAKGGKMPLVGGLFYLLLIGEVPTKE
                     QALEVEAEWAKRANVPDYVFKMLKTMPKETHPMTLFSQAVLALQNESVFAAKYHSMKK
                     DQYWEAALEDSLDLTAKLPIIAAYIYRMKFFGETSKPKYNPKQDYGLNFARMMKVSDK
                     KGYADLARLYFILHSDHESGNVSAHTMHLVGSALSDPYLAFSAALNGLAGPLHGLANQ
                     ECLGWLLSVKEKFGGVPSRDDLYKFSWDTLNSGQVIPGYGHAVLRVPDPRFTAQMEFA
                     KKRFPDDELVRLADMVFDVVPAVLKEQGKAKNPAPNVDAISGTLQHYYGVREFDFYTV
                     LFGVGRALGVTPNYVWMRALGMPIERPKSLTTKMLEDVVAKAGQPAA"
     gene            complement(1869..3944)
                     /locus_tag="PKI33_01970"
     CDS             complement(1869..3944)
                     /locus_tag="PKI33_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNM35795.1"
                     /translation="MPTNKHHPTAPKRPHPLTSHGVTRMDDYYWMRDRNDPETMKYLK
                     AESDYLEEVMQHTKSTQEVLFAEMKARIQETDSTVPEKRGEYFHYERFEKGNQYPIFC
                     RKKGSLEAKEEVILDQNQLAEGKPFCSISGIQTSPDGSKLAYLADFEGNEVYTLYIKN
                     LETGAYFSETIGNVSGSAYERIGVDWANDNQTLFYITLDETLRPHKLYRHILGTDPKN
                     DVLVLEEADEEYYLFCYKSRDGELITTYHMSTNTREVRFMDANDPTSELRILQPRIEG
                     LDYSAAHHKGKFFIVHNDNAKNFRLSVVSAETPGKEHWVDVLPHREDTLVEYVETFEH
                     HIVLHERKGGLKQIRISDTDGKSNAHYVQFPEASYSVYPEGNSEFATTSLRLKYSSLV
                     TPFTVVDVDMNTGKWDIKKVEEIPSGLDKDKYTLERVLATSHDGTQVPMTIAYRNDMQ
                     KKDGTNPALMYGYGSYGATIDPYFDSNRFSLIDRGFVYAIAHIRGGYDMGRDWYEQGK
                     MENKRNTFLDFIACGEYLIHESYVAKDKLAIQGGSAGGLLVGACMTMRPDLFKAVIAK
                     VPFMDVVTTMEDPSIPLTTQEYTQWGNPADKKFFEYMLSYSPYDNLRATDYPNLLITT
                     GLNDPRVAFWEPAKFTAKLRELKTDDNLVVFYVNYDSGHAGASGRFDYIKEIALDYAF
                     LMDRLNIKS"
     gene            complement(4005..4535)
                     /locus_tag="PKI33_01975"
     CDS             complement(4005..4535)
                     /locus_tag="PKI33_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM35796.1"
                     /translation="MSHISPPESQEYAPFYSGYIQLASARGDLTAALSLQPDEMKAAM
                     GGLSDAQARFKPGPAEWSIKEVVSHLIDGERVFSYRLLRVSRKDKTPLPGFEQDDYVR
                     EAKADEIALADLIDEFTFLRRANLLAIKNMSASALAEVGTASGNPVSARALVYILVGH
                     VEHHMASLKEKYLPFA"
     gene            4602..4907
                     /locus_tag="PKI33_01980"
     CDS             4602..4907
                     /locus_tag="PKI33_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35797.1"
                     /translation="MNFTPLYKHLFRPPSPTDRLNLFQTLFDSNEINPDLALALLKIV
                     HSELSSEEGDGASYRQYAQIIEALRYHNMDMLRHIVETWKAGKPAKDPEWLSDGKIK"
     gene            4948..>5624
                     /locus_tag="PKI33_01985"
     CDS             4948..>5624
                     /locus_tag="PKI33_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNM35798.1"
                     /translation="MSITIGTAVAKRGEIVYGEYFLVQHPVGGADVLPVIIAQGHEDG
                     PVFWITAGVHGIEHAGIQVIHKLITPELVNQLRGTIVAIPALNPAGLRTLERQAYYHA
                     GDPNRLFPDGRAPKTDPDKMPPSALEQAYGRLFEEIKKTASFYFDLHCMGGHALSFIF
                     RDRVLYRKGDEEARKRAEIVSQKMDEMARAYGHTMTADFALGKYIDEKLHRATTASVS
                     MLAGIPS"
BASE COUNT         1376 a         1323 c         1421 g         1504 t
ORIGIN      
        1 gttgggcggt catggcgatg aaatggttcc gcttactcgt cactccaaca ttgcaggcgt
       61 gccgctcaag gaatatttcc cggctgataa gctcgaagcg atcgttaacc gcacccgcaa
      121 aggcggcggt gagatcgtga acttgctcaa gacgggttct gcctattatg cgccttccat
      181 ggcttgtgtt cagatggcag aagctattct gaaggataag aaactcatcg tcccttgcgc
      241 tacgttgatg cagggtgaat atggtttgaa cgatatgtac ttcggtgtgc ccgtcatgtt
      301 gggtgcaggc ggcatggaaa aagtgatcga atacaagttt gatgccgacg aaaaggcaat
      361 gttcgagaaa tcggctgctt ctgttcgaga atcacacgac gccctcaaga gcgttgtgag
      421 tctataacca tcaaaggtca aaagtcgaag gtcagaatgg ctttcgactt ttgtattgaa
      481 aatcaatagg agactcccat gattttgcac gaaaaaatgt ctgcccaatt gcctgcctgg
      541 cgcgaacgca ttaagagcct tgctaaagaa cacggcgaag ttgtggttga taaagttacc
      601 gtcggtcaga tcgttggcgg tatgcgcgat atcaaatccc tgctcaccga tgtctctttt
      661 gtagacccgg ctgaaggcat tcgcttccgc ggtatgtcta ttccagaagt gctaaaggcg
      721 ctgcccaagg caaagggcgg caagatgcct ttggtgggtg gcttgttcta ccttctgttg
      781 attggtgaag tgcctaccaa ggaacaagcc cttgaagtgg aagcggaatg ggcaaaacgc
      841 gcgaacgtgc ctgattatgt cttcaagatg ttgaagacca tgccgaaaga aactcacccc
      901 atgacgctct tctcgcaagc ggtgctcgct ttgcagaatg agtcggtctt tgcggcgaag
      961 tatcacagca tgaagaaaga tcaatattgg gaagccgctc tcgaagatag tctcgacctc
     1021 acggcgaagc ttcctatcat tgcggcgtat atctaccgca tgaaattctt cggcgaaacc
     1081 agcaagccga aatacaatcc caaacaagat tatggtttga acttcgcccg tatgatgaag
     1141 gtttcggata agaaaggcta cgccgacctt gcccgcttgt acttcatcct acacagtgac
     1201 catgagtctg gaaacgtttc ggctcatact atgcatttgg ttgggtctgc cctctctgac
     1261 ccttacctcg ccttttccgc cgcgctcaat ggtttagcag gtccgttgca cggacttgcc
     1321 aaccaagaat gtttgggctg gcttctcagc gtgaaagaga aatttggcgg cgtcccgtct
     1381 cgcgatgacc tctacaaatt ctcttgggat accctcaata gtggacaggt catccctgga
     1441 tatggtcacg ctgtgttgcg tgtgccagac ccacgcttta cggcgcagat ggaatttgcc
     1501 aagaagcgct tccccgacga tgaattggta cgccttgccg atatggtctt cgacgtggtc
     1561 cctgctgtgt tgaaggaaca aggcaaagcc aaaaatcctg ccccgaacgt agatgccatt
     1621 agcggcacgc ttcaacatta ctatggtgtg cgtgaattcg atttctacac ggtcttattt
     1681 ggtgtaggtc gcgccctcgg tgtgaccccc aactatgtgt ggatgcgcgc gcttggtatg
     1741 cccatcgaac ggcccaagtc tctaacgacc aagatgcttg aagatgtggt cgccaaagcg
     1801 ggtcaacctg ctgcgtaaag tttgataaaa aagaccttcg ctattagcga aggtcttttt
     1861 ttatttaatt atgatttgat gtttaagcga tccatcaaaa aggcgtaatc taatgcgatt
     1921 tctttgatgt aatcgaaacg tccacttgcc ccggcgtgac ctgagtcata gttgacgtaa
     1981 aagactacca gattgtcgtc tgtctttagc tcgcgtaact tcgctgtgaa cttggcaggc
     2041 tcccaaaatg ccacacgtgg atcgttcaac ccagtcgtaa tgagcaggtt gggataatct
     2101 gtagcgcgaa gattatcgta cggcgaatat gaaagcatat attcgaaaaa ctttttatct
     2161 gccgggttgc cccattgagt atattcctgc gtggtcaacg gaatgctcgg gtcttccatg
     2221 gtagtgacca catccataaa gggcactttg gcgatgacgg ctttgaacaa atcaggtcgc
     2281 atggtcatgc aggcacctac gagcaatcca cctgcagacc ccccttgaat ggcgagcttg
     2341 tctttggcaa cataactttc gtggatgaga tattcaccgc aagcgataaa gtctaaaaag
     2401 gtattgcgct tgttttccat cttaccttgt tcgtaccaat cacgccccat gtcatacccg
     2461 ccgcggatgt gagcaatggc atacacaaat ccacggtcaa ttaaactaaa gcggttcgag
     2521 tcaaaataag gatcaatggt cgcaccgtac gagccgtatc catacatcaa tgctgggttg
     2581 gtaccgtctt tcttttgcat atcgtttcgg taagcaatgg tcatgggaac ttgggttcca
     2641 tcgtgagaag tggcaagcac acgttctaat gtgtatttat ctttatctaa cccacttggg
     2701 atctcttcta ccttcttaat atcccatttg ccggtgttca tatccacgtc aacgacagta
     2761 aagggtgtga ccaaggaaga atatttcaga cgcaatgaag ttgtggcaaa ctctgaattt
     2821 ccttcagggt aaacactgta cgaagcctca ggaaattgca catagtgagc gttgcttttc
     2881 ccatctgtat cgctaatacg aatttgtttc agcccgccct tgcgttcatg taaaacgata
     2941 tgatgttcaa aggtctctac atactctact aaagtatctt ctcgatgagg aagaacatcg
     3001 acccaatgtt ctttgccggg tgtctctgca gaaacaacgc tcagcctgaa gtttttcgca
     3061 ttgtcattat ggacgataaa gaactttcct ttatggtgtg cagcactata gtctaaccct
     3121 tcaatgcgcg gctggagaat acgcagttca cttgtcgggt cattcgcatc cataaagcgg
     3181 acttcacgag tgttggtgct catgtgatag gtagtgatta gttcgccatc acgagatttg
     3241 tagcaaaaaa gataatactc ttcatctgct tcttctaaaa ccaaaacatc attcttgggg
     3301 tccgtcccaa ggatgtggcg atataactta tgcgggcgca gggtttcatc caacgtgatg
     3361 taaaagagag tttgattgtc attcgcccag tccacgccga tgcgctcgta ggcactgcct
     3421 gaaacattgc cgatcgtttc ggagaagtaa gccccggtct caaggttttt gatgtatagc
     3481 gtgtacacct cattgccttc gaaatctgcg agataggcaa gcttgcttcc atcgggactg
     3541 gtttgaattc cgctgatgct gcaaaacggt ttgccttctg ctaattgatt ttgatctaag
     3601 atgacctctt cttttgcctc gagagaacct tttttacgac aaaagatcgg atactgattc
     3661 cccttttcga atcgttcgta gtggaaatac tcgcctcgtt tctccgggac ggttgaatca
     3721 gtttcctgaa tccttgcctt catttcggcg aagagcactt cctgcgtaga cttggtatgc
     3781 tgcatgactt cttcaagata gtcactttcg gctttgaggt atttcatggt ttctgggtca
     3841 ttgcggtcgc gcatccagta ataatcgtcc atgcgggtga cgccatggct ggttaacggg
     3901 tgtggtcgtt taggagcagt ggggtggtgt ttgttggtag gcatgaaatt gtccttaaga
     3961 acaaaaagtg acagcccttg gtctatggac tgtcactgaa aaaattaagc aaagggtaaa
     4021 tacttttctt tcaggctagc catgtgatgc tccacatgac caacaagtat atagaccaag
     4081 gctcgggcgc tgacggggtt tccgctggcg gtaccaactt cggcgagggc agaggcgctc
     4141 atgttcttga tggcaagcaa gttggcgcgg cgtagaaaag taaattcatc gatcagatct
     4201 gcaagggcga tctcatcagc tttggcttcg cggacgtagt catcttgctc aaagccgggg
     4261 aggggagttt tatccttgcg ggaaacacgc agcaaacgat aggagaagac ccgttcgcca
     4321 tctatcagat gagatacgac ttctttgatg gaccattctg caggtccggg tttgaagcgg
     4381 gcttgtgcgt cagatagccc tcccatcgca gctttcattt catctggttg aagcgacagg
     4441 gcggcggtta aatccccgcg tgcgctggca agttggatgt aaccggaata aaaaggggca
     4501 tactcttgtg attcaggtgg ggaaatgtgg ctcatatggg ctcctattta ggattatatc
     4561 gtaccgataa ttcttttatt cactgtataa tacagggacg catgaacttt actccacttt
     4621 ataaacattt gtttcgcccg ccttctccca ccgatcggtt gaatttattt cagaccttgt
     4681 tcgattcgaa cgagatcaac ccagatctgg cattggcttt gctgaagatc gtccattcgg
     4741 agttaagctc tgaggaaggt gatggggcgt cgtatcggca atatgcccag atcattgaag
     4801 ccttgcgtta tcacaacatg gacatgttgc ggcatatcgt agaaacctgg aaggcgggga
     4861 aacctgccaa agaccccgag tggctttcag acgggaagat taaataaact cttttgtttg
     4921 ggggtgagtt aacggaggaa acgtttcatg tctatcacca tcggcactgc cgttgccaaa
     4981 cgcggcgaga tcgtgtacgg cgaatatttt ttggtgcaac atcccgtggg tggcgcggac
     5041 gttctgccgg ttatcattgc gcagggacat gaagacggac ctgtgttttg gatcacggcg
     5101 ggggttcatg gcattgaaca tgcaggcatt caagtcattc ataaattgat aacacctgaa
     5161 ttagtgaatc aattgcgcgg aacaattgtc gctatcccgg ctttgaaccc cgctggcttg
     5221 cggacgttgg aacgccaagc ctactatcac gccggtgatc cgaatcgcct cttccctgat
     5281 ggacgcgctc ccaagaccga ccctgacaaa atgccgccct cggcattgga acaggcatat
     5341 ggacgtttgt ttgaagagat caaaaagacc gcttcatttt attttgacct gcattgcatg
     5401 ggtggacatg cactttcatt tattttccgc gaccgcgtac tgtatcgcaa aggggatgaa
     5461 gaagcccgca aacgcgccga gatcgtgagc caaaaaatgg acgagatggc gcgggcgtat
     5521 ggtcacacca tgacggcgga ttttgcgttg ggcaaatata ttgacgagaa attacatcgc
     5581 gccaccaccg cctccgtttc gatgttggcg ggcatccctt cgtt
//
LOCUS       DAOREF010000060         5554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8020_length_5554_cov_4.115657, whole genome shotgun sequence.
ACCESSION   DAOREF010000060 DAOREF010000000
VERSION     DAOREF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8020_length_5554_cov_4.115657"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKI33_01990"
     CDS             <1..342
                     /locus_tag="PKI33_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNM35799.1"
                     /translation="ETRKILEGALHPMLEKRIDTVVLGCTHYPFVIPLIEKIVGENVR
                     VIDPAPAVAKQVKRLLEAGEALNQKEQLGSTRFMTSGDVEVVKSSLKILLQLEREVEA
                     VTWLNDREIAA"
     gene            complement(308..799)
                     /locus_tag="PKI33_01995"
     CDS             complement(308..799)
                     /locus_tag="PKI33_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNM35800.1"
                     /translation="MLEKRPNSLLKFFFKTPLFMHKLGLGGWERLIGAQWMFLTTIGR
                     KTGKCRETLVDVMDYDSATDTYFIEAAYGTRADWYKNIQAHPVFEAQVGRRKFNASAK
                     ELNSQDTGETLVQFFRKKPAYTRSVMAMVGLKFKDENELRALGDKLTLLAVTPLSHDR
                     SAK"
     gene            complement(792..1562)
                     /locus_tag="PKI33_02000"
     CDS             complement(792..1562)
                     /locus_tag="PKI33_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNM35801.1"
                     /translation="MQIKGLDTLARHVPELNSSSGRMKIGATFFAIFVLTTFYFIITD
                     QIPTWTLDSQIVVTALGYLILSRVFRQKKAFIEKHKEQAFAVAFKQFTVPSLAIFSAL
                     IIHIAYMNGPKFTQPTLTTILTWLGWVCILIGTPLFIRALTILGADHATMLYVYFPGG
                     ELTHSSIYNIVRHPMYAGMLRICIGLACLNRGIFALTFVIILPILVFGWIRLIEEKEL
                     IERIPNYIEYRKQVPAFFPYPKQIFRFFKFVLTGQSYA"
     gene            complement(1595..2089)
                     /locus_tag="PKI33_02005"
     CDS             complement(1595..2089)
                     /locus_tag="PKI33_02005"
                     /inference="COORDINATES: protein motif:HMM:NF023629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3601 domain-containing protein"
                     /protein_id="HNM35802.1"
                     /translation="MNRSTPNPLFTALLLFVSFIFFALVMWIMDNPFPTSWNIIAVLM
                     GLFFITFLWGFLRILRARDIKNSQLIQSRPSAPPPLPTPETEGTVYGLYPGWEYIVVQ
                     SFTDYYGNIFEVNQKLKFKGRHFLPYQGGHTIMFEECSLYLQEDQNKNILDNFSSYIV
                     KIKP"
     gene            complement(2095..2709)
                     /gene="cyaB"
                     /locus_tag="PKI33_02010"
     CDS             complement(2095..2709)
                     /gene="cyaB"
                     /locus_tag="PKI33_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNM35803.1"
                     /translation="MSGQETEVKFFVQDLKRVEMRLQELEAHLIQPRVHEVNLRFDNE
                     AGELRKNFKVLRLRQDIEAKFTFKGPSEERAGGVLSRKEIEFSVGSFEAAKEFLELLG
                     FVPVVFYEKYRTTYEFLGVHIMLDELPYGTFVEIEGEDIEALQKVANLLGLNWNAMVR
                     AGYHALFERITEKYNLEYGQLSFEALKSIIATAEDFGITYSDIN"
     gene            complement(2712..3209)
                     /locus_tag="PKI33_02015"
     CDS             complement(2712..3209)
                     /locus_tag="PKI33_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNM35804.1"
                     /translation="MKYITTIEDKQFEIEVVDDHHVRVGGRLLEVNFESVSGQPVYSL
                     ILDGKSYESYVYPGDEDWQVLLRGRQFQAKVEDEREKRLKAAAGGGVAEGGEFHLKAP
                     MPGLVVAIPIAEGQEVKKGEVLVILESMKMQNELKSPRDGTVSHIKVKAGESVEQKQV
                     LLNLH"
     gene            complement(3227..4735)
                     /gene="accC"
                     /locus_tag="PKI33_02020"
     CDS             complement(3227..4735)
                     /gene="accC"
                     /locus_tag="PKI33_02020"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNM35805.1"
                     /translation="MFKKVLVANRGEIAVRILRACRELGLETVAVFSEADRNALHVRY
                     ADEAYLLGPAPSRESYLRTDKILDVVKRSGADAIHPGYGFLAEREDFAAKCADLGITF
                     IGPKPSAIAAMGDKAEARATVIKAGVPVVPGTEDVGNMTNDDLLRIAPTIGFPLLIKA
                     TAGGGGKGMREVRSLEEMPTLLESARREAESAFGDGNVYLEKLIEGARHIEFQIIADE
                     HGNVIHLGERECSIQRRHQKLVEEAPSPFLDEELRKQMGDVAVKAAQAVDYVNAGTIE
                     FLVDKDRNFYFLEMNTRLQVEHPVTELVTGIDIVAEQLRIARGRQLSYTQEQVQFKGH
                     AIECRVNAEDPFNNFIPSTGRITHSIIPTGPGVRVDTGVYPGFEITPYYDPMIAKLIV
                     WGETRAQAILRMRRALEEYRIVGVRTNIPFHQTLMDSHRFMGGQFDTRFVEERFSMEA
                     AATLDEAQAEIAAILATLVSHKETERSAQIVRGNERDTSNWKWVGRWERMHR"
     gene            complement(4754..>5554)
                     /locus_tag="PKI33_02025"
     CDS             complement(4754..>5554)
                     /locus_tag="PKI33_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNM35806.1"
                     /translation="NMEDSPAAPTSDDPLRMEEALNSLVPDDANKPYDIKEVIRLIVD
                     DGNFFEIHENYAANIVVGFARLGGQSVGIVANQPAVLAGVLDIDASEKGARFVRFCDA
                     FNIPIITFEDVPGFLPGTFQEHHGIIRSGAKLLYAYCEATVPKLTVITRKAYGGAYCV
                     MSSKHIRSDLNLAWPTSEIAVMGPDGAVNIIFRRELEAAEDPVKRKAELVAEYKEKFA
                     NPYVAASRGYIDDVIEPKETRPRLINALAMLSNKRDSNPAKKHGNIPL"
BASE COUNT         1422 a         1277 c         1356 g         1499 t
ORIGIN      
        1 gaaactcgca aaatcttaga aggtgctctt caccccatgc ttgaaaagag gatcgatacc
       61 gttgtgttgg gatgcactca ttaccctttt gtgattccgt tgatcgaaaa aatagtgggt
      121 gaaaacgtgc gggtgataga cccagctccg gcagtggcaa aacaggtcaa acgtctgctt
      181 gaggcgggcg aagctttgaa ccaaaaggag caacttggga gcacacgctt tatgacatcg
      241 ggtgacgtcg aggtggtcaa atccagtttg aaaattttgt tgcagcttga gcgggaagtc
      301 gaagcggtta cttggctgaa cgatcgtgag atagcggcgt gaccgctaag agagttagct
      361 tgtcgcctaa tgcgcgaagc tcattctcat ctttgaattt caacccgacc attgccatca
      421 cggagcgggt ataagcaggc ttttttctaa aaaactgaac caacgtttcg ccagtgtctt
      481 gtgagtttaa ctctttagca ctggcgttaa attttcgcct accgacctgt gcttcaaaaa
      541 ctgggtgcgc ttgaatgttc ttataccaat ctgcacgggt gccataggcg gcttcaatga
      601 agtaggtgtc tgtggcagag tcatagtcca tcacatctac aagcgtttcg cggcatttgc
      661 cggtttttct gccgatggtg gtgagaaaca tccattgcgc cccaatgagg cgctcccaac
      721 cacctaaacc gagtttgtgc ataaagagag gagttttgaa gaaaaacttc agtagagagt
      781 tggggcgttt ttcaagcata cgattgacct gttagaacaa atttgaaaaa gcggaatatt
      841 tgtttcgggt agggaaagaa tgccggcacc tgttttcgat attcaatata gttgggaatg
      901 cgttctatta gttccttttc ttctatgaga cggatccaac caaaaacaag gatgggtaga
      961 ataattacaa aggtcagcgc aaaaattccg cggttgaggc acgctaaccc aatgcagatg
     1021 cgaagcatgc ctgcatacat ggggtgacga acaatgttgt agatacttga gtgtgtcagc
     1081 tcgccgcctg gaaaatacac atatagcata gtggcatgat ctgcacccaa tatggtaagc
     1141 gcacgaataa atagtggcgt tccaataaga atacaaaccc agccaagcca agtgaggatt
     1201 gttgtaagtg taggctgggt aaattttggt ccattcatgt aagcgatgtg aataattaat
     1261 gcagaaaaaa tagcaagact tggcactgta aattgtttga atgccacggc aaaggcttgc
     1321 tctttgtgct tttcaataaa agctttcttt tgcctgaaga ctcgactaag aataagataa
     1381 cccaatgcag tgacgacaat ttggctatca agagtccatg tggggatttg gtcggtgatg
     1441 atgaaataaa aggtggttaa aacaaatatg gcgaagaatg ttgcgccgat tttcatccgc
     1501 cctgatgatg aatttaattc tggaacatgc cttgctaaag tatctaaacc tttgatttgc
     1561 atggcagcct ccttgttcga aacattgaaa cgatttatgg tttgattttg acaatataac
     1621 tcgaaaaatt atcgagaata ttcttgtttt ggtcctcttg taaataaagg ctacattctt
     1681 caaacataat cgtatgcccg ccttgatacg gtaaaaaatg gcgtcctttg aatttgagct
     1741 tttgattcac ctcaaagata ttgccatagt agtctgtaaa agattgtact actatgtatt
     1801 cccagcctgg gtaaagtcca tagacagttc cctctgtttc tggagttggt aaaggtggcg
     1861 gagcggacgg tctggattga ataagttgag aattttttat gtctcttgct cgtaagatgc
     1921 gcaagaatcc ccataaaaac gtgatgaaaa acagccccat gagaacggcg atgatgttcc
     1981 atgaagtagg gaatggattg tccatgatcc acatcaccag ggcaaagaaa ataaaactga
     2041 caaaaagcaa tagagcagtg aagagcgggt taggcgtaga acggttcata aattctagtt
     2101 tatatcagaa taggtgattc cgaaatcttc agccgtggca atgatggact ttaatgcctc
     2161 aaaactcaac tgaccatatt caaggttgta cttttctgta atgcgctcaa acagtgcgtg
     2221 ataccccgcc ctgaccatag cgttccagtt caacccaaga agattcgcta ccttttgaag
     2281 tgcttcaata tcttcgcctt cgatttcaac aaaagtacca taaggcaatt catctaacat
     2341 aatgtgcacg ccaagaaatt cataggttgt gcgatacttt tcataaaaga caacaggcac
     2401 gaaaccaagt aattctagaa attctttggc tgcttcaaaa ctgccaacag agaattcgat
     2461 ctctttccgg ctgagtacgc cacccgcgcg ttcttcactt ggacctttga acgtgaattt
     2521 ggcttcgata tcctgtcgca ggcgaagtac cttgaagttt tttcttaatt ccccggcttc
     2581 attatcgaat cgtaaattga cttcatggac acgcggctga attaaatgcg cctccagttc
     2641 ttgaaggcgc atttcaactc gttttaggtc ttgcacaaaa aacttaactt cggtttcttg
     2701 tccgctcatg gttagtgtag attcagcaac acttgctttt gctcaacgct ctcgccagcc
     2761 ttgaccttga tatggctcac cgtgccatcg cgcggtgact ttagctcatt ctgcatcttc
     2821 attgactcaa ggataacaag tacctctccc tttttgactt cttgcccttc ggcaatagga
     2881 atggcgacaa ccagccctgg cattggcgct ttgagatgga actcgcctcc ttctgccacg
     2941 cctccgcctg cagccgcttt cagtcgcttt tctctttcat cctcaacttt ggcttggaat
     3001 tgtcgtccgc gtagtaagac ctgccagtct tcatcaccgg ggtatacata cgactcgtag
     3061 gatttgccat cgagaattaa agaatagaca ggctgaccgc taaccgattc aaagttgact
     3121 tcgagcaggc gtccgcccac acgtacatgg tggtcgtcga caacttctat ttcaaattgc
     3181 ttatcttcta ttgtagttat atatttcatg ccatagcctc ttctaattag cggtgcatgc
     3241 gttcccagcg accgacccac ttccagttgc tggtgtcgcg ctcgttacca cggacgatct
     3301 gagcagagcg ttccgtctct ttgtgagata cgagcgtagc gaggatggct gcaatctctg
     3361 cctgtgcttc atccaaggtg gcagcggctt ccatggagaa gcgttcttcc acgaagcggg
     3421 tgtcgaattg cccacccatg aaacgatgcg agtccatcaa ggtttggtgg aaggggatat
     3481 tagtgcgaac acccacgatg cgatattctt ccagagcacg gcgcatgcgc aaaatggctt
     3541 gagcgcgtgt ctcgccccaa acaatcagtt tggcaatcat tggatcgtaa tatggtgtga
     3601 tttcaaaacc ggggtacaca cctgtgtcca cacgaacgcc gggtccagta gggatgatgc
     3661 tgtgggtaat gcgacccgtg gatgggatga aattgttaaa cgggtcttct gcattgacac
     3721 ggcactcaat cgcatgtccc ttgaactgta cttgttcctg tgtatagctg agttgacgtc
     3781 cacgggcgat gcgcaactgt tcagcgacga tgtcgattcc ggtgaccaac tctgtcacag
     3841 gatgttccac ttgcaaacgg gtgttcattt caaggaagta gaaatttcgg tctttatcaa
     3901 ctaggaattc aatcgtccct gcgttgacat agtccacagc ttgcgcagct ttgacagcta
     3961 catcgcccat ttgcttgcgc aattcttcat cgagaaaggg tgagggcgct tcttctacca
     4021 atttttgatg gcgacgttga atggaacatt cacgttcgcc caagtggatg acattgccat
     4081 gttcatcagc aatgatttgg aattcgatgt gacgtgcgcc ttcaatcaat ttctcaagat
     4141 agacgttgcc atcgccaaat gcagattctg cctcgcgcct cgcggactca agaagagttg
     4201 gcatttcttc caggcttcgg acttctctca tgccttttcc gccgccaccc gctgtcgcct
     4261 tgataagaag cgggaagcca atcgtaggag caatgcggag taagtcatcg ttggtcatat
     4321 tgccaacgtc ttcggtgcct ggcacaacag gtacgccagc tttgatgact gtagcgcgtg
     4381 cttcggcttt gtcgcccatc gccgcaatag ctgaaggttt gggtccaata aaggtgatgc
     4441 caaggtctgc gcatttggcg gcgaaatctt cgcgctctgc aaggaagccg taccccgggt
     4501 gaatagcatc tgccccagag cgttttacaa catcgagaat cttatctgtg cgtaaatagg
     4561 attcacgtga aggagcggga ccgagtagat aggcttcatc cgcatagcgc acatgaagtg
     4621 cgttgcggtc tgcttcggaa aatacggcaa cggtttcaag ccctaattcg cgacaagcgc
     4681 gcaagatgcg gacggcgatc tcgccacggt ttgcaaccaa tactttttta aacatgattc
     4741 tctcctcgcc tgattacagc ggaatgttgc cgtgtttctt ggctgggttt gaatcgcgtt
     4801 tgttgctcaa catggcaagt gcgttgatca agcgcggacg ggtttcttta ggttcgataa
     4861 catcatcgat atacccacga gaggctgcaa catacgggtt cgcaaatttc tctttatatt
     4921 cagcaaccaa ttcagccttg cgcttgacgg ggtcttctgc ggcttccaac tcgcggcgga
     4981 agataatgtt gactgcacca tcgggaccca tcacggcgat ttcggaggtg ggccaggcga
     5041 ggttgaggtc actgcggatg tgtttggaag acatcacgca atacgcaccg ccatacgctt
     5101 tgcgagtgat gaccgttaac ttcggtacag tcgcttcgca atatgcataa aggagttttg
     5161 ctcccgagcg gataatgcca tgatgttctt ggaatgttcc gggcaggaag ccgggcacat
     5221 cttcaaaggt gatgatcggg atgttgaatg cgtcgcagaa gcgaacgaag cgtgcgcctt
     5281 tttcactggc atcaatatca agcacacctg cgaggacagc aggctgattg gcaacgatgc
     5341 caacggactg tccgccgagg cgggcgaaac caaccacaat attggcggcg tagttttcat
     5401 ggatttcaaa gaagttacca tcgtccacaa taaggcggat gacttctttg atgtcgtaag
     5461 gtttattggc atcatctggt acaagtgaat tgagtgcttc ttccatgcga agcggatcat
     5521 ccgatgttgg tgccgcaggt gagtcttcca tgtt
//
LOCUS       DAOREF010000061         5532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8073_length_5532_cov_4.892825, whole genome shotgun sequence.
ACCESSION   DAOREF010000061 DAOREF010000000
VERSION     DAOREF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8073_length_5532_cov_4.892825"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1005..1316
                     /locus_tag="PKI33_02030"
     CDS             1005..1316
                     /locus_tag="PKI33_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNM35807.1"
                     /translation="MTTLSQHDGRPEDELIQNLLAAGLTHYYESEELWKKWEILSPRE
                     KEITALVCLGYTNKQAAERLVLSPETVKSHLRNVLIKFNLSTRSELRMALEDWDFSAW
                     T"
     gene            1440..1973
                     /locus_tag="PKI33_02035"
     CDS             1440..1973
                     /locus_tag="PKI33_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35808.1"
                     /translation="MNIIPYLKNNRFVVVLSLHSAKEYIPTLIAELALRGPVTVLDAG
                     NQFQAYRIVQLIRLRSLHVDDIAKRIAVRRAFTCYQTVSLLAETPAQPHPYVILDLLA
                     SFYDDQVSILECRRLLTICIKEIERLSFTAPVVLDLPPIRVEAKSFLMDELCRRADEV
                     FILETLPAIQEKQLALF"
     gene            1980..2243
                     /locus_tag="PKI33_02040"
     CDS             1980..2243
                     /locus_tag="PKI33_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35809.1"
                     /translation="MGRTVVTITQLLNETEASLSPFRRSLRRSDQYIFDGILASARRH
                     ITAISQTDSLLPFEAALLAMLLEQAKEIAVLRQELDQLMGKDG"
     gene            2236..4371
                     /locus_tag="PKI33_02045"
     CDS             2236..4371
                     /locus_tag="PKI33_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNM35810.1"
                     /translation="MDKVNGWLFDLYEHPEYGLVLWVIADSGERLRLRMEFPTVFYVA
                     GDFPLLRQAWVFLRKESTKLERVTRRDLFIGDREVMQVTLHKQSSLIHELQRQFPSLD
                     YYDADIPIALRFIAETDVHLLGRCRVILNGEWVKTIEPLDSPWQLSPNRIPLRIMELA
                     PDADPAFRAPTRLKVRYEAVEYVMNIEPHRALLISLQADLRRFDPDLILTRHGDPWLI
                     PMLIQWSQETGLGLDLNRDKHCNVLQKKANSYFTYGQVVHRRAQSHLFGRWHIDRKNA
                     MLFSEYELEGVFEQARVTGIGVQEMARKSPGAGITAMQMQTALKNKVMVPLHKQQAED
                     RKSLAELIRADKGGLVYQPLIGVHKDVAQIDFSSMYPGIMVHYNVSPETIHKTNPEEG
                     LIPKTLKPLLEKRLAIKKLLTELDLRDCEIPVLKQRSDALKWLLVVCFGYLGYKNARF
                     GKIESHETVTAISRELMLQAKEIAEDMGFDVLHMYVDSLFVRKDGLKNKSDFDTLLKM
                     IAEQTRIPIALEGIYRWVTFLPSRRDKRVPVPNRYFGVFQNGDVKYRGIEARRRDTPY
                     WVRKIQLEVLDILAKAEALEEIPTWLPYTSDHVARAKRDLRNGNIPLEELVITQSLSR
                     AVEEYKVPSPAAQAGMQLQLAGKLVAPGQFMRFVYVYGKERVRAWELGVDPRTVDMKR
                     YCLLLDRAVTTVIEEIKTEPQSFFCNPVL"
     gene            4869..>5532
                     /locus_tag="PKI33_02050"
     CDS             4869..>5532
                     /locus_tag="PKI33_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNM35811.1"
                     /translation="MNSLQRIKNVISGKPVDRTPNLDIFMIRAVHHLGIPLSKYYLDY
                     RVLCEVNMAVLKDFELDVVQAISDPYREACDMGLEVEFPYDKMPMHRNLVIHEPEDLA
                     KIKFPVQDFGSRMTDRLEGVRAMREQVGDEVAVMGWVEGAFAEAADLRGMTDIMKDLY
                     MRPNWLKELLEKCAEVEIAFASAQIDAGAHMIGLGDAASSLISPRMYREFALPYQQRI
                     FEA"
BASE COUNT         1451 a         1227 c         1393 g         1461 t
ORIGIN      
        1 aagtatcaca ccattttctg gaaaaatagc caagattgaa ttgtttgctt aaccctatgt
       61 attataagtt gcgccttttt aatgttgagg gacaaatggc cccctgaaga gaccaaaagt
      121 acctttgtcg gacaatttat gaaatttgtc gatgttttag gtggaaatgg gattgctctt
      181 cgaagttggg agagtttccc caaggcatga atattgctat gtccaaaaat cgagagaaca
      241 tattctgctg gcgttacgac ccgcacagga gagaagtgat gttctagagc atatagaaga
      301 aacgggagac ccttcccttg ctgtagaggc tgggacgcaa ttgcaactga cagggaagac
      361 ggttgctcct ggggagttta tgaggttcgt ttatgtgtta ggaatggaat aggttcgtgc
      421 ttggggattg ggagtggatt ctcgtactgt ggatttgaag agatattgtt tgttattgga
      481 tcgggcagtg aatacagtga agggagaaat aaagactgag tcacagtttg tttctataac
      541 ccagctttta tatctcttag agaaagactt actcaaaata tatctacgag tttttattag
      601 gcaatccagg taaaacaaca aaccttgcag ttagttcaat agtatatcca atgtgactac
      661 ttgatggata caatgttaga agacgcttga gaagaatatt taatcatgtg gtgatgatac
      721 tatttttatt accaaacaat tgatcattgc tagttggaat taaagatagt aaggttctct
      781 tgggtgatga ttaatgaatc ttgatttgaa tgagaattgg gataataacg agaaaaaata
      841 ttcaagattt tctagcagcc aaaatcatat tcaatttgac aacagaacac ttgttctatt
      901 acaatcggcg catgtctatc tggcacaaac ttttggccgt gataggcttg cgccccttat
      961 ccgcccctcg gaaatatcag gtcagtgagt ccatgcaagt aactttgacc accctctccc
     1021 agcacgacgg gcgtcctgaa gacgagttga tccagaacct gctggccgca ggcctcaccc
     1081 actactatga atccgaagaa ctatggaaga aatgggaaat cctttcgccg cgcgagaagg
     1141 agattaccgc tttggtgtgt ctcgggtata ccaacaaaca ggctgcggag cgtttggtcc
     1201 tctcccctga aaccgtaaaa tcacacctgc gcaatgtcct gatcaagttc aacctgagca
     1261 ctcggtccga gcttaggatg gcgcttgagg attgggattt cagcgcttgg acatagatgt
     1321 gctggttcgt tccatatcta tacccacata ttcatccccc gctcggttaa acgatattca
     1381 acctccaaat cccccatcag gggatttttt atattcttgc ctactgttat tgtgttggca
     1441 tgaacatcat cccttactta aaaaataaca gattcgtagt ggttctttca ttacatagcg
     1501 ccaaagaata catcccaacc ctgatcgccg agcttgccct gcgcggaccc gtcactgtat
     1561 tggatgccgg taatcaattc caggcgtatc gcattgtaca actcatccgc ctacgatccc
     1621 tgcatgtgga tgacattgcc aaacggattg cagtgcggcg ggctttcacc tgctaccaaa
     1681 ccgtcagcct gttagccgaa acaccggctc aaccccaccc gtacgtgata ctggacctgc
     1741 tggcttcttt ttacgacgat caggtctcca tcctcgagtg ccgaagattg ttaactatct
     1801 gtatcaaaga gatcgagcgg ctaagcttta cggcacctgt cgtactcgac ctgcctccca
     1861 ttcgggtgga ggcaaaatca tttctcatgg acgaactatg caggcgggca gatgaagtct
     1921 ttatattgga aacacttccc gcgatacaag aaaaacaact ggctttgttt taggtgaata
     1981 tgggacgaac cgtagttacg atcacgcaac tgctcaatga gacggaagca tcgctttcac
     2041 ccttccgccg ctccctgcgg cggagcgatc agtacatctt tgacggcatc ctcgcctctg
     2101 cacggagaca tatcactgcc attagccaga cggattcact tcttcctttt gaggcggcat
     2161 tgttggcgat gctgcttgag caggcaaagg agattgctgt cctgcggcag gagcttgacc
     2221 agttgatggg taaagatgga taaggtcaac ggctggctct tcgatcttta tgaacatcct
     2281 gaatacggtt tggttttgtg ggtcatcgcc gacagtggtg aacgcctgcg gttgaggatg
     2341 gaatttccaa ccgtttttta tgttgcgggc gatttcccgc tgctgcgcca ggcgtgggtt
     2401 ttccttcgga aggagagtac gaaattggag cgggtaacac gccgcgatct ttttatcggg
     2461 gatcgtgaag taatgcaggt cacgcttcat aaacaaagta gtctgatcca tgaattgcag
     2521 cgtcaatttc cttccttgga ttattacgat gccgacattc ccatcgcgtt gcggttcatt
     2581 gcagaaacag atgttcacct gcttggtcgt tgccgggtca ttctaaacgg ggagtgggtt
     2641 aaaaccattg agcctttgga ttcgccctgg cagttatcgc ccaatcgcat cccattgcgc
     2701 atcatggaat tggctccaga cgccgaccct gcctttcgtg cgccgacccg gttgaaggtc
     2761 cgctatgagg cggtggaata tgtgatgaat atcgagccgc atcgggcatt gctcatcagt
     2821 ctgcaagccg acttgcggcg cttcgatcca gatctcattc tcacccgcca tggtgatccc
     2881 tggctgatac caatgttgat tcaatggtct caagagacgg ggttgggatt ggatctgaac
     2941 cgtgacaaac attgcaatgt cctgcaaaag aaagctaaca gctactttac ctatgggcaa
     3001 gtggtgcatc gccgcgcgca gagtcatttg tttgggcgct ggcatattga ccgcaagaac
     3061 gcaatgttgt ttagtgaata tgaactggag ggagtcttcg aacaggcgcg agtgaccggc
     3121 attggagttc aggagatggc acgcaagtct ccgggggctg gcattactgc aatgcagatg
     3181 cagaccgcgt taaagaacaa ggtaatggtt cctctgcata aacaacaagc tgaggatagg
     3241 aagagccttg ctgagttaat ccgcgccgat aaaggcgggc tggtctacca acctttgatc
     3301 ggggtgcata aagatgtagc ccagattgat ttctcttcga tgtacccggg catcatggtg
     3361 cattacaacg tctctccaga aacgatccac aagacaaatc cagaagaggg gttgattcca
     3421 aaaaccttga agcccctgtt ggaaaagcga ttggcgatca aaaagttatt gaccgaatta
     3481 gatctgcgcg attgcgaaat ccccgtcctc aagcaaagat cggatgcatt gaagtggctg
     3541 ctggtggttt gctttggata tttgggatat aagaacgcca ggttcgggaa gatcgaatct
     3601 catgaaactg ttacagccat cagccgcgag ttgatgctgc aagccaaaga gattgccgag
     3661 gatatggggt tcgatgtcct tcatatgtat gttgatagcc tctttgtgcg gaaggatggt
     3721 ttgaaaaaca agagcgattt tgataccctg ctaaaaatga tcgcagaaca aacccgaatc
     3781 ccgattgcac ttgagggaat ttatcgttgg gtgactttcc ttccatcccg tagggacaag
     3841 cgggtgccgg ttccaaatcg ttactttggt gtcttccaaa atggagatgt gaaataccgc
     3901 ggcattgaag caaggcgcag agatacaccc tattgggtgc gaaaaattca attggaagtg
     3961 ctggatattc tggcaaaggc ggaggcactg gaagagatac caacctggct gccatacaca
     4021 agtgatcatg ttgcgcgtgc aaaacgcgac cttcgaaatg ggaacatccc gttggaagaa
     4081 ttggtgatca cccaaagcct gagccgcgct gtggaagaat acaaagtccc ttcgcctgct
     4141 gcacaagccg gaatgcaatt gcaactggca gggaaactgg ttgccccagg acaatttatg
     4201 aggttcgttt atgtatacgg caaggagagg gtccgtgcct gggagttggg ggtagatccc
     4261 cgcactgtgg atatgaagag gtattgtttg ttattggacc gggcagtgac cacggtgata
     4321 gaagaaataa aaactgagcc acagtctttt ttctgtaacc cagttttata aatcattagg
     4381 gaaagcttac ttattacatt tgtctatgga cttttagtag gcaatacagg taaaacaacg
     4441 aacctcgcgg tcagttcaat agtgtatcga gtgtgactac ttgatgaagg cgatgttcgg
     4501 atttgcttga gcggtgacag aagaagggtt cataattttt tacacaaaaa acggcatgtt
     4561 tttcgtcaat gaaattgctg ttttatgcaa gtttcctttc gtgtgttgtg ttctcttgat
     4621 actacatgat accttagacg atactttggg gcaattaaat ctcacattaa aaatgtgagg
     4681 gcaaaggcgg caaagttgac atgaagtgca aacggacgca catcccgttc ggttttctga
     4741 tgcattcatt tgcattgttg ctcaaagagt gtaaggcgcc ggtacttcaa aataaagttg
     4801 aattgctata ttatttgcta tcatgtagtt tcaaacaaga aacacatcca atctatggag
     4861 gtaattgcat gaactcactg caacgcataa aaaatgtcat ctccggcaag ccggtggacc
     4921 gcactcccaa cctcgatatt ttcatgattc gcgccgtgca tcacctcggc atccccttat
     4981 cgaaatatta tctcgattac cgcgtgttat gcgaagtgaa catggctgtc ttgaaagact
     5041 ttgagctgga tgttgtgcaa gccatctcag acccatatcg tgaagcctgc gacatgggct
     5101 tggaagttga atttccatac gacaaaatgc ccatgcatag gaacctggtt atccatgagc
     5161 cggaggatct tgccaagatc aaattcccgg tgcaagattt tggttcgcgc atgacagacc
     5221 gcctggaggg tgtccgtgcg atgcgtgaac aggtgggaga tgaagttgca gtgatgggct
     5281 gggtggaagg cgcctttgca gaagccgcag acctgcgcgg tatgaccgat atcatgaagg
     5341 atttatacat gcgccccaac tggttgaaag aattgcttga aaaatgcgca gaagttgaga
     5401 tcgctttcgc ctctgcccaa attgacgctg gagcgcacat gattgggctg ggcgacgctg
     5461 catcctctct catttcaccg cgcatgtatc gcgaatttgc cctcccctat caacaacgca
     5521 tctttgaagc cg
//
LOCUS       DAOREF010000062         5529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8080_length_5529_cov_4.857691, whole genome shotgun sequence.
ACCESSION   DAOREF010000062 DAOREF010000000
VERSION     DAOREF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8080_length_5529_cov_4.857691"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKI33_02055"
     CDS             complement(<1..516)
                     /locus_tag="PKI33_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35812.1"
                     /translation="MNFDYGNILTRALKLTWKHKSFWLLLMIPVFISFMILTAIILPV
                     IFLTDNEESITLIMVLWAGIVIFGFFASFIIGAVSMNAVTLGILRAERGEGSTALMDV
                     VRDSFQYFKNAFGAVLIVQLSVGAVFTVFFLCMTALTLVTMGIASICLQPIMLLLTPL
                     SFLVGAVLYGAL"
     gene            complement(549..2084)
                     /gene="guaA"
                     /locus_tag="PKI33_02060"
     CDS             complement(549..2084)
                     /gene="guaA"
                     /locus_tag="PKI33_02060"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNM35813.1"
                     /translation="MNSIAILDFGSQYAQIIARRVRESQVYCELFPWDAPQEKILSIK
                     PKGFILSGGPKSVYEENAPFIQKFILESGLPILGICYGMQALTHALGGKVDPSAQREY
                     GHAEITPLVPGTMLDVLSKVWMSHGDKVTQLPEGFMALAKSGNSPFAAMGDMKRKYFG
                     VQFHPEVHHTPNGTELIKHFAIDICGVTPDWTPANIIEESVKRIREQVGNERVLAAVS
                     GGVDSSVAAALVHKAIGDQLVCVFVDTGLLRANEGAQVASAFREGLGAELITVDAADD
                     FLGALKGQTDPEMKRKIVGEKFIRTFEREAKNVGQPRFLVQGTIYPDVVESSGPDRSK
                     AAKIKSHHNVGGLPEDMQFELVEPLRYLFKDEVRLVGEALGLNSGLVWRQPFPGPGLT
                     VRCLGECTPERVARLRAADAILLEELSKAGFLGKGAQTSQAFVVLLPVRSVGVMGDMR
                     TYQEAAAIRAVATDDFMTADWARLPFELLAKVSSRIVNEVEGINRVVYDITSKPPATI
                     EWE"
     gene            complement(2118..2465)
                     /locus_tag="PKI33_02065"
     CDS             complement(2118..2465)
                     /locus_tag="PKI33_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNM35814.1"
                     /translation="MIRKILHLRFIVFLIGWIFAHMSFAIPVKRLRETSNLIAFQHPS
                     PSYKVHLLITPKRQVQSLAELDPQDTAFFTDLYSTVQSLVKELDLKAYRLIVNGGEYQ
                     DFPHLHFHLISEM"
     gene            complement(2499..3068)
                     /gene="xpt"
                     /locus_tag="PKI33_02070"
     CDS             complement(2499..3068)
                     /gene="xpt"
                     /locus_tag="PKI33_02070"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNM35815.1"
                     /translation="MNALQERILKEGKVLGGGILKVDSFVNHQVDPVLMDECGRAFAK
                     LFAKVGATKILTAEISGIAPALTTGIHMGLPVVYARKTKPVTMPDQVYLTLAPSHTKG
                     RMVELIVSPEYLANNEKVLIIDDFLASGQTILGLVRLAEASGSKVVGIGALVEKSFEG
                     GRDALASVGVQIESIACIKSLDDGKIEFV"
     gene            3253..3894
                     /locus_tag="PKI33_02075"
     CDS             3253..3894
                     /locus_tag="PKI33_02075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNM35816.1"
                     /translation="MASNSPRRRQLFGLGGWEFTVIVADVDETPFEHEPPRDYVIRLA
                     QAKASAVQPKAGLDAVILGSDTTVVDGNEILGKPVDESDAWRMLKQLRGRTHQVYTAI
                     AILQHGKMYTDLSMVDVPMREYSDQEIDTYIKTGDPMDKAGAYAIQHPEFKPVSSLEG
                     CRAGVMGLPMCHVVRTLKHFNISSFADVPSACQSLLQYDCKVSPLILNGEIVN"
     gene            3913..>5529
                     /locus_tag="PKI33_02080"
     CDS             3913..>5529
                     /locus_tag="PKI33_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNM35817.1"
                     /translation="MKSLRWKQLALVALIIVGCTGPGSGSGVGSIFATDTPLPTPSVG
                     ITPAPNVDSAVTAFFDALKGDNYEEMYAMLSQSSRDALTLEDFSKRWTNALNEMSASS
                     FDVTVLSKSITPFNAEVKYSITYKTALVGDIQREMLMRLTNEDSNWKVQWDDALILPE
                     LAGGNLLKMEYSIPARGDIYDSKGLPIATQSDAFAFGIQTDQIDYDMIGALTTELGRL
                     CGLDPLDIQAQIDAVGPGWYLPMCEGTREEAQRLLSINPGGLVIQEYNSRYYFRGGLA
                     PQAVGYTLAISPEQLNQYKRLGYSGSERIGYTGVEQWAEDYLAGKHGGILRVVSPTGQ
                     VIATLGQSQPAPADSVYLTLDSNMQYYAQAAVEYFRGAIVVMEVDTGRIIAMASGPDF
                     DPNVFDSNNRNNQAVGNIFNDTRNPLLNRAAQGQLPLGSVFKIITFSAALESGLFLKD
                     TTYDCQYDFTELQDQVRHDWTWQHCQDRLAGGLFCNTSDSTPSGLLTLQEGLMRSCNP
                     YFWHIGNDLFTNWDRGNDIANMARAFGLGSP"
BASE COUNT         1456 a         1304 c         1378 g         1391 t
ORIGIN      
        1 caacgctcca tagaggacgg cacccactaa aaatgatagg ggagtgagca aaagcatgat
       61 cggttgtagg cagatggatg caatacccat ggtgacaagg gtgagagccg tcatacagag
      121 aaagaaaact gtaaatacgg cacccacact gagttggacg ataagaactg caccaaaagc
      181 attcttgaaa tattgaaaac tatcgcggac tacatccata agcgcagtag aaccttcacc
      241 gcgctcggct cgcaaaatgc caagtgtgac tgcattcata ctaactgcgc cgataataaa
      301 actagcaaaa aaaccaaaaa tgacaatccc agcccaaagc accatgatca aggttataga
      361 ttcttcgttg tcagttaaaa agatgacagg caaaatgatt gctgtgagaa tcatgaaaga
      421 aatgaacacg ggaatcatca agagaagcca aaacgattta tgcttccagg tgagctttaa
      481 tgcgcgggtt aagatattgc catagtcgaa gttcatttcc atctcccaat tgtcaatcga
      541 aaattgattt actcccactc aatggttgca ggtggtttgc tcgttatatc atagaccaca
      601 cgattaatgc cttctacttc attgacaata cggctcgaga cctttgctaa taattcaaat
      661 ggtaagcgag cccaatcggc ggtcatgaag tcatccgttg ctacagcgcg gatcgctgct
      721 gcttcttgat aggttcgcat gtcacccatc acgcccaccg aacgaaccgg caataacaca
      781 acaaatgcct gtgacgtttg agcgcccttg cccagaaaac ctgctttgga taattcctcc
      841 agaaggatcg catctgccgc ccgcagacgt gccacacgct cgggcgtaca ctccccgagg
      901 cagcgcacgg tcagaccggg accggggaac ggttgacgcc acaccaaacc agaattcaag
      961 ccgagggctt cgcccaccaa acggacctcg tccttgaata aatagcgcaa cggttccacg
     1021 agttcaaatt gcatatcttc agggagtccg cccacattgt gatgagactt gatcttggct
     1081 gctttgctgc ggtcgggacc ggatgactca actacatcgg ggtagatcgt gccttgcact
     1141 aagaatctag gttgacctac attcttggct tcgcgttcaa aggtgcggat aaatttctcg
     1201 ccgacaattt tgcgcttcat ctctgggtca gtttgcccct tcaatgcgcc gagaaaatcg
     1261 tcagcggcat cgacggtgat gagttcagca cccaagcctt cccgaaatgc cgaagcgact
     1321 tgtgcgcctt cattggcgcg caagagtccc gtatccacga aaacacagac cagttgatcg
     1381 ccaatcgctt tatgaactaa tgccgctgca actgaggaat ctacgccacc ggaaacggct
     1441 gcgagcacac gttcgttgcc cacttgttca cgaattcgtt ttacgctttc ttcgatgatg
     1501 ttagcggggg tccaatcggg cgttacgcca caaatgtcga tggcgaagtg cttgatgagt
     1561 tcggtgccgt tcggggtgtg atgcacttcg gggtgaaatt gcacaccaaa atatttgcgt
     1621 ttcatatcgc ccatcgccgc gaagggactg tttccacttt tggcgagtgc catgaaacct
     1681 tcggggagtt gtgtcacttt atcgccgtgt gacatccaaa ctttggagag cacatccaac
     1741 atggtgccgg gaaccaatgg cgtgatttcg gcatgaccat actcgcgctg ggcagatggg
     1801 tccactttgc cgccgagggc gtgggtgagg gcttgcatgc cataacaaat gccaaggatg
     1861 ggaagcccgc tttcgaggat gaatttttga atgaagggcg cattctcttc ataaacagac
     1921 ttgggtccgc cagataaaat aaatcccttc ggttttatcg aaaggatttt ttcttgcggc
     1981 gcatcccatg gaaagagttc acaatagact tgtgactcgc gcacacggcg ggcaataatt
     2041 tgggcgtact gagaaccgaa atctaaaatg gcgattgaat tcatgtgttt tattaatccc
     2101 gtagggggga ctcaccccta catttctgaa ataagatgaa aatgtaaatg tggaaaatct
     2161 tgatactctc cgccattgac gattagccga tacgctttga ggtcaagttc ttttacgagg
     2221 ctttgcacag tggagtataa gtctgtaaag aaagcggtgt cttgtgggtc aagctctgcc
     2281 aacgattgaa cctgtcgctt cggggtgata agcaaatgaa ccttataact aggagaagga
     2341 tgctggaacg caatcaggtt cgaggtctca cggaggcgtt tgaccgggat ggcgaaactc
     2401 atgtgagcga agatccaacc gatgaggaaa acaatgaagc gcaggtgtag gattttacga
     2461 atcacggttc actctttgca aagagtgaac cgtgtgtgtt agacaaactc aatcttgcca
     2521 tcatccagtg acttgataca agcgatggat tcaatttgca caccaacaga agcgagagca
     2581 tcacgcccgc cttcgaaact cttttcgacc aacgcgccaa tgccaacgac tttggaacca
     2641 gaggcttctg ccaaacgtac aagcccaaga atggtctgtc cgctggcgag gaagtcatcg
     2701 atgatgagaa ctttttcgtt gttggcaaga tattccggtg agacgatcaa ctccaccatg
     2761 cgacctttgg tgtgagaagg ggcgagggtg agatacacct gatctggcat ggtaacgggt
     2821 ttggtcttgc gagcatacac aacgggcaaa cccatgtgaa tgccagtggt gagcgccgga
     2881 gcaatgcccg agatttctgc tgtgagaatt ttggttgcac ccacttttgc aaagagtttg
     2941 gcaaacgcac gcccgcactc atccatgagc acagggtcta cttggtgatt aacaaaacta
     3001 tctactttaa gaattccacc gccgagcact ttcccctctt ttaaaattcg ttcttgcaaa
     3061 gcattcatga agcctccgat tgtctggaca gatatcgaat gattttacaa ccggatttga
     3121 aaagagagaa gataaatgac aaaaaaagtt gtctgagtac tttgccaata tttccaacga
     3181 cttatcgcgg tttaatccat caaaaatgac tgtgttagaa tcttgctctt gatggataaa
     3241 gtaaagttcg ttttggcatc taattctccg cgcaggcggc aactatttgg cttgggcggg
     3301 tgggagttca ccgtcatcgt tgcagatgtg gatgagactc cgtttgagca tgagcctcca
     3361 cgggattatg tgatcagatt ggcacaagcc aaagcttcag cggttcaacc caaggcgggg
     3421 ctggatgcgg ttattctggg gtcagatact acggtggtag atgggaacga gatcctcggg
     3481 aaacctgtcg atgaatctga tgcgtggcgc atgttaaagc aattgcgcgg gcggacgcat
     3541 caggtctaca ccgcgattgc catccttcaa catggaaaga tgtatacaga cttatccatg
     3601 gttgatgtgc ccatgcgcga atactcagac caagaaattg acacctatat caaaaccggc
     3661 gaccccatgg ataaagctgg tgcatatgcc attcaacacc cggagtttaa gccggtctct
     3721 tcattggaag gttgccgtgc cggggtgatg ggcttgccta tgtgtcatgt ggtgcgcact
     3781 ttgaaacact ttaatatttc atcttttgca gatgttccat ctgcatgcca atctctttta
     3841 cagtatgatt gtaaggtctc gccgttgatt ttgaacggtg agatcgtcaa ttagaaattg
     3901 aaaggaaacg taatgaagtc attacgctgg aaacaattag ctctcgttgc tttaatcatt
     3961 gtcggctgta ccggacctgg ctcagggagt ggagtcggtt cgatttttgc aaccgataca
     4021 cccttgccaa ccccgtcggt tggaatcact cccgcgccta acgtggattc agcagtcact
     4081 gcgttttttg atgcgctcaa aggtgacaat tacgaagaga tgtatgccat gttgtcacag
     4141 tccagccgcg atgcgttgac cttggaagat ttctcaaagc gttggacgaa tgctctaaat
     4201 gaaatgagcg catccagttt tgatgtgacc gttctctcaa aatccattac gccttttaat
     4261 gcagaagtta aatacagtat tacgtataag actgcccttg ttggcgacat tcagcgtgaa
     4321 atgttgatgc gccttacgaa tgaagatagc aattggaaag tgcaatggga cgacgccctg
     4381 atccttcctg aattggctgg tggcaactta ctaaagatgg aatatagcat ccctgcccgt
     4441 ggggatatct acgatagcaa aggcttgcca attgccactc aatctgatgc gtttgctttt
     4501 gggattcaaa ccgatcaaat tgactatgac atgatcggcg cattgacaac cgaacttggg
     4561 cgtttatgtg gcttggaccc gcttgatata caggcgcaga tcgatgctgt tggccccggt
     4621 tggtatctgc cgatgtgcga agggacgcgc gaagaagccc agcgtttact ctctattaac
     4681 cctggcggtt tggtcattca agaatataac tcacgctatt acttccgcgg tgggttggct
     4741 ccgcaagccg tgggatacac cctggcgatt tctccagaac aacttaatca atacaagcgt
     4801 ttaggctata gtggtagtga gcgaattggt tataccggcg ttgagcaatg ggcagaagat
     4861 tatttagccg gcaaacatgg cggcatttta cgtgttgtta gcccaacggg gcaggtgata
     4921 gccaccttgg ggcaaagtca acctgcgccc gctgattcgg tatatctcac tctcgatagc
     4981 aatatgcaat actacgctca ggcggcagtg gaatatttcc gtggtgccat tgtagtgatg
     5041 gaagtcgata ccgggcgtat catcgctatg gcatcgggac ctgattttga cccgaatgtt
     5101 tttgatagca ataaccgcaa taaccaagct gtagggaata tttttaatga cacgagaaac
     5161 ccgcttctga accgtgctgc tcagggtcaa ctcccattag ggtctgtttt caagatcatt
     5221 accttctccg cagccctcga aagcggactg ttcttaaaag atacaacata cgattgtcag
     5281 tatgatttta cagaactaca agaccaagtg cggcatgact ggacgtggca gcactgtcag
     5341 gaccgtcttg ctggcgggtt attctgtaac acgtctgata gcaccccctc gggtttgctt
     5401 actttacaag aaggtctaat gcgttcttgt aacccatact tctggcacat tggcaacgac
     5461 ctcttcacaa actgggaccg tggcaacgac attgcgaaca tggcacgtgc atttggtttg
     5521 ggttctccc
//
LOCUS       DAOREF010000063         5501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8131_length_5501_cov_4.154058, whole genome shotgun sequence.
ACCESSION   DAOREF010000063 DAOREF010000000
VERSION     DAOREF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8131_length_5501_cov_4.154058"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1363
                     /gene="tgt"
                     /locus_tag="PKI33_02085"
     CDS             236..1363
                     /gene="tgt"
                     /locus_tag="PKI33_02085"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNM35818.1"
                     /translation="MFDFSITAKNNRARTGVFSTPHGDLITPVFAPVGTQATVKTLTP
                     EHLKSINASLVLSNTYHLYLRPGDELVRDMGGLHKFMQWHNPMLTDSGGFQVFSLSQT
                     RKVDEDGVTFKSHIDGSTHRFTPEKSIAIQENLGADIIMAFDECSDPNDHAYIKKAME
                     RTHRWAERSLKAKTRSDQALFGIVQGGVDPTLRAESAKFIASLETPGIAIGGLSVGET
                     KQEMHDTLDVVTPLLPENKPRYLMGVGTPEDLINGVLRGVDIFDCVLPTRLARHHSAF
                     APEGRLNMMNASFARDERPIDETCDCYACQTFTRAYIRHLIVAKEMLASTLVSIHNLR
                     ALIRLMENIRVYIAEGSFEDRAPELLLQWGNNAKRVVNGVK"
     gene            1364..2176
                     /gene="uppP"
                     /locus_tag="PKI33_02090"
     CDS             1364..2176
                     /gene="uppP"
                     /locus_tag="PKI33_02090"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNM35819.1"
                     /translation="MNIFHAIVLGIVQGLTEFIPVSSTAHLFIVSNLLGLPSDDQVFA
                     FDVIIQLGTVGALLLFFWHDYWDIAKAVFEGIKNRRPFEDYHSRLGWLVIVATIPAGV
                     VGLALKPLVQQMSGDPLLWGGIRLLFTSALLAAVEYFDKKSRTLESATWVDAFSVGLF
                     QVLAIIPGASRSGSTMAGAMFRGFDRPSAARFAFLMSAPILLAAGLYETIQVILLPNT
                     TEFLPILLTGFLVSAIVGWLAIRWLMEYLRKHSLYVFSVYCLIVGVIVLALK"
     gene            2198..3283
                     /locus_tag="PKI33_02095"
     CDS             2198..3283
                     /locus_tag="PKI33_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNM35820.1"
                     /translation="MDTKSLLKLLVETESPSHEKSAVDRVGAIVADEARKLGAEIEIV
                     SNKETGDHVIARWRGEGKPILLVCHMDTVFPLGTIEKFPYQEEEGKIKGPGVSDMKAS
                     IAISLGAIEEAYNNGAKRAITLLCTSDEEIGSITSRELIQNMAKEAELVFVMEPALLD
                     GSLKTWRKGGGGFKVKVHGRAAHAGGDHEKGRNAIEEMAHQVLAIQKMTDYSKKTTLN
                     VGVIHGGTVSNVVPDYAEIDIDVRILQPEEWGRVEGMMMALKPVLDGTTVEVTGSLNR
                     GPMPFDELMQATFGKAKSIAAEIGIDLKAGGTGGVSDANLVAPLGIPVLDGLGAIGEG
                     YHSEREYIFAGSVAQRVKLISALLVNW"
     gene            3293..3931
                     /locus_tag="PKI33_02100"
     CDS             3293..3931
                     /locus_tag="PKI33_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35821.1"
                     /translation="MAVPYNKKIIFVCFSFFLFSCAPKPLSSNQIITAYSTASAQPWM
                     NDLFACADELSVAVKVAADSPDIYLRIGEPDDVLTPVFQIDEEELLIVTHRESPVQNL
                     SLEEAQALFAGSSGLAVQVWVYPSELDVYEVFDQFVMQGRSVISAARVAVNPQEMSDV
                     LNQESNAVGILPRHWKAGNVREVYSVGIFPVLAVTQEEPQGNVAQLIACLQK"
     gene            4028..4678
                     /locus_tag="PKI33_02105"
     CDS             4028..4678
                     /locus_tag="PKI33_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35822.1"
                     /translation="MNQDFNEIQQRVRHSWFKDGVGEIAVGGLFVALSLYFAGHYFAG
                     TRFLPSASNAPLYIDLGLGVVLLLGIVLTRKIIAVFKTHITYPRTGYVEYYIESKNSL
                     LVRVAIFIAGTLFVLLLIWVGRWVGSFKWIPAFLGVFFGGVLLFLRTKTAGLKRFNYH
                     ASLSILLGLGLTFSSMPIPGLSVYYAVFGVWLLGWGIWILAKYLRENPLPEGDEDE"
     gene            4671..4982
                     /locus_tag="PKI33_02110"
     CDS             4671..4982
                     /locus_tag="PKI33_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNM35823.1"
                     /translation="MNDDLRSLTDLDRMIHEPARLLLVTILSGVASADFLFLQRQTGL
                     TKGNLSAHLSRLEEAGYVKIEKTFKGKMPFTVCKLTSAGQKALQRYRQQVQNFMDKTR
                     S"
     gene            5055..>5501
                     /locus_tag="PKI33_02115"
     CDS             5055..>5501
                     /locus_tag="PKI33_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM35824.1"
                     /translation="MNTPLIELKNVSKTYSTAAGEFPALRNINLNVYAGEFLGVIGKS
                     GAGKSTLLNMINGVDELSEGEVIVNSNNSRVSVHNLSEDEKALWRGKTMGVVYQSFQL
                     LPMLTLVENITLAMDLADNFKPRESRERAMELLRLVEIEEHADKLPS"
BASE COUNT         1352 a         1102 c         1420 g         1627 t
ORIGIN      
        1 gcgctttagg ggtggaggtt tgtgtaggag cggcagttgc gggcgcggca caagctgtca
       61 gcaacaggat caaaaatagg ctatgggtga ggcggggttt tgtcatgatt tctctctcta
      121 agaataagtc tattattgca attatatgaa acggtctatg cctgaaaaag agccaattga
      181 ggggcagttt gcataccaat tcggtgatac actttcatcc tgtataattt gccccatgtt
      241 tgatttcagc attacagcta aaaataaccg cgcccgcacc ggggttttct ccacgccgca
      301 cggcgatctc attactcctg tgtttgcacc agtgggtaca caagccactg tcaaaacact
      361 gacgcctgaa catctcaaga gcattaacgc ttcactcgtc ttgagcaata cctatcattt
      421 gtatctgcgc cctggcgatg aattggtgcg cgatatgggc gggttgcata aattcatgca
      481 gtggcataac cccatgctta ctgattcggg tggctttcag gttttttctc tttcgcaaac
      541 tcgcaaagtg gatgaagatg gcgtgacctt caagagtcat atcgatgggt ctacgcatcg
      601 ttttacgcca gagaagtcga ttgcgattca agagaatctc ggcgccgaca tcatcatggc
      661 atttgatgaa tgttctgacc cgaatgacca tgcatatatc aagaaagcca tggagcggac
      721 tcatcgttgg gcggagcgtt cgctgaaagc aaagacgcgc tcagaccaag ccttgttcgg
      781 gatcgtccaa ggtggagtgg atcctacgct gcgagcggag tctgccaagt tcatcgcctc
      841 gttagagaca cctggcattg cgattggtgg tttatctgtt ggtgaaacca agcaagagat
      901 gcatgatacg cttgatgtgg ttacgccgtt gttgcctgag aataagccgc gctatctgat
      961 gggcgttggc acacctgaag acctcatcaa cggtgtcttg cggggtgtag atattttcga
     1021 ttgtgttttg ccaacccgtt tggcgcgcca tcattctgct tttgcaccgg aaggtcgcct
     1081 taatatgatg aatgcttcct ttgcgcgcga tgaacgccct attgatgaaa cctgtgactg
     1141 ttatgcttgt cagactttta cacgggcgta cattcgtcac ttgattgtgg caaaggaaat
     1201 gcttgccagt actttggttt ccatccataa tttgcgggcg ttaatccgct taatggagaa
     1261 tatccgcgtt tatattgcgg agggctcatt tgaagatcgt gcgccagagt tgttgttgca
     1321 gtgggggaat aatgcgaagc gagtagttaa cggagttaag taattgaata tttttcatgc
     1381 aattgttttg gggattgtac aagggttgac cgaattcatc cctgtttctt ctacggctca
     1441 cttgtttatt gtgagcaatt tacttggtct tccgtcagat gaccaagtat ttgcatttga
     1501 tgtgattatt caattaggaa cagtaggagc attgcttcta ttcttttggc atgattattg
     1561 ggatatagcc aaagctgttt ttgaaggtat caaaaaccga agaccctttg aagattatca
     1621 ttctcgtttg gggtggttgg tgattgtagc aactattcct gcaggggtgg tggggttggc
     1681 gcttaagccg ttggttcaac aaatgtctgg cgacccactg ttatggggcg gaatacgatt
     1741 gttattcact tcagcgttat tggcggctgt cgaatatttt gataaaaaga gtcgcacgct
     1801 tgaatcagca acttgggtgg atgctttttc ggttgggcta tttcaggtat tagcgatcat
     1861 tcccggcgcc tcccgttccg gctcgacaat ggcgggtgca atgttccgtg gttttgatcg
     1921 accctctgcg gcgcgctttg ctttcctcat gtctgcaccc attctgttgg ctgctggctt
     1981 gtatgaaacg atccaagtta tactcttacc aaacaccact gaatttttgc ctattttgct
     2041 tacaggtttt ttggtttcgg ctattgtggg ctggcttgct attcgttggt tgatggagta
     2101 tttgcgaaaa cattctttgt atgtcttttc tgtttattgt ttgattgttg gtgtgattgt
     2161 tttggcgttg aaataaaatt ttgaattgga gctgaccatg gataccaagt ctcttctaaa
     2221 acttcttgta gaaacagaat ctccctcgca cgaaaaatct gccgtggacc gtgtcggtgc
     2281 tattgtggca gatgaagctc gtaaacttgg tgcggagatt gagatcgttt cgaacaaaga
     2341 gacaggtgac catgtgattg cacgttggag aggggaagga aaacccattt tgttggtctg
     2401 tcatatggat acagtttttc cattagggac gattgaaaaa tttccgtatc aagaggaaga
     2461 aggcaagatc aaaggaccag gcgtctctga tatgaaagcc agcattgcta tttctctcgg
     2521 tgcaatcgaa gaggcatata acaatggagc aaaacgagct attacgttgc tgtgtacttc
     2581 agatgaagag atcggcagta ttacatctcg cgaactaatt caaaacatgg caaaggaagc
     2641 tgaactcgtt tttgtgatgg aacctgcttt gctggatggt tctctcaaga cttggcgtaa
     2701 agggggcggc ggatttaagg tcaaggttca tggacgggct gcgcatgcag gcggtgacca
     2761 tgaaaaaggg cggaatgcga ttgaagaaat ggcacatcaa gtacttgcta ttcaaaagat
     2821 gacggattac tccaaaaaga ccacattgaa tgtgggggtc atccacggtg ggacagtttc
     2881 gaatgtcgtg ccagattatg cggagattga tattgatgtc cgcattttgc aaccggaaga
     2941 gtggggacgg gtggaaggaa tgatgatggc tttgaaacca gtcctggatg gcacaactgt
     3001 ggaagtgaca gggtcactca accgtggacc aatgccattc gatgagttaa tgcaggctac
     3061 ctttggaaaa gccaagtcca ttgctgctga gatcggcatt gacctcaaag cgggtggtac
     3121 aggcggcgtc tctgatgcga atttggtcgc gccattagga attccggtct tagatggtct
     3181 tggtgcgatt ggcgaaggct atcattcaga gcgcgaatac atttttgcag gtagtgtggc
     3241 tcaacgtgta aagttgatct cggcattgct tgtcaattgg taatcatttt ctatggctgt
     3301 tccatataac aagaagatta tttttgtttg tttttccttc tttttgtttt cctgtgcacc
     3361 aaaaccatta tcttcaaatc agattatcac agcgtattcc acagcatctg cacagccatg
     3421 gatgaatgac ttatttgcct gtgcggacga actgtctgtt gcggtgaaag ttgccgcaga
     3481 ctcgcccgat atttatttgc gtatcggcga gccagacgat gtactaaccc ctgtatttca
     3541 aattgatgaa gaagaactac tcattgtcac gcatcgtgaa agcccggtgc aaaacctttc
     3601 tttagaagaa gctcaagctt tattcgctgg ttcaagtgga ttggcggtac aggtttgggt
     3661 gtatccatca gagttggatg tgtatgaggt gtttgaccag ttcgtgatgc aaggacgaag
     3721 cgtgatatct gcagctcggg tggcggtgaa tccgcaagag atgtctgatg tgttaaatca
     3781 agaatcgaat gcggtcggaa tcctgccgcg acattggaaa gcggggaatg tgcgtgaggt
     3841 gtattctgtg gggatttttc ccgttcttgc tgtgactcaa gaagagccgc aggggaatgt
     3901 tgctcaatta atcgcttgtt tgcagaaata aaattctcat cttagtttta tttttcactt
     3961 gacgcctcct aatgggatgg tattatctaa accggtttgt agtgcaaacc ggtttgtgtg
     4021 gagtattatg aatcaagact ttaacgaaat tcaacagcgt gtgcggcatt cctggtttaa
     4081 ggatggtgtg ggcgaaattg cagtgggcgg attatttgta gcactttcac tatattttgc
     4141 cgggcactac tttgctggta cacgcttttt gccttcggct tccaatgcgc cgttgtacat
     4201 tgaccttggt ttgggagttg ttcttctttt aggcatcgtg ctaacacgca agatcatcgc
     4261 tgtgttcaag actcatatta cgtatccacg tacagggtat gtagaatact acattgaatc
     4321 taagaactct ttattggtac gagttgctat ttttatcgca ggtacattat ttgttctgtt
     4381 attgatttgg gttggaagat gggtggggtc tttcaagtgg attcccgctt ttctgggcgt
     4441 tttctttggc ggagtcttgt tatttctgcg cacaaagaca gctggcttga aacgttttaa
     4501 ctatcatgca agtttgtcca tcttattagg acttggacta acatttagca gtatgcctat
     4561 ccctgggttg agtgtgtact acgctgtttt tggcgtttgg ctgttaggtt gggggatttg
     4621 gatcttggca aagtatttgc gtgagaaccc actgccagag ggagatgaag atgaatgatg
     4681 acctccgttc actaaccgat ctagatcgta tgattcacga accggctcgg ctcttattgg
     4741 taaccattct ctccggcgtt gccagcgccg attttctctt tctccaacgc cagaccggtt
     4801 tgaccaaagg taacctgtct gcccatcttt cgcgcttgga agaagctggg tatgtgaaga
     4861 tagaaaagac ctttaaagga aaaatgcctt tcacagtttg taagctgacc agtgctgggc
     4921 aaaaggcgtt acagagatat cgccaacagg tacaaaattt catggataag acccgttcgt
     4981 gagcaattga aaactctttg caaataaagg acacaaaatc gcatcaacaa cagcggtaaa
     5041 atgaaattga acttatgaat acaccattga ttgaattgaa gaatgtatca aaaacatatt
     5101 ctacggcggc aggcgaattc cccgctttgc ggaatatcaa cctaaacgtc tatgcgggcg
     5161 aatttttagg ggtaattggc aaatcaggtg cgggaaaatc taccttgttg aacatgatca
     5221 acggagtaga tgaactgagc gaaggcgaag tgatcgtcaa ctcgaacaat tctcgtgttt
     5281 ctgtgcataa cctgagtgaa gatgaaaaag cactctggcg cggtaaaact atgggcgttg
     5341 tataccagtc ctttcagctt ctgcccatgt taaccctggt cgagaatatc acccttgcta
     5401 tggacctggc agataatttc aaaccccgcg aatcgcgtga acgcgctatg gaacttttgc
     5461 gtttggtaga gatcgaagaa catgccgata aactcccgtc a
//
LOCUS       DAOREF010000064         5466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8215_length_5466_cov_3.794123, whole genome shotgun sequence.
ACCESSION   DAOREF010000064 DAOREF010000000
VERSION     DAOREF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_5466_cov_3.794123"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKI33_02120"
     CDS             <1..416
                     /locus_tag="PKI33_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35825.1"
                     /translation="FIILVAAQLSVVLANLVVLFIALGSVWVIVPIFFWAHGVFLKRQ
                     NVLTSILSSFQLTRFTLPTSSLFVLTVFLLSYGLNYLWRLPSLNSWLTLLGIFGHSFV
                     TTALLAGSFIYYYEMSAWVQAVLEKLRPNNVIKQA"
     gene            456..2375
                     /gene="gyrB"
                     /locus_tag="PKI33_02125"
     CDS             456..2375
                     /gene="gyrB"
                     /locus_tag="PKI33_02125"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNM35826.1"
                     /translation="MAENNYDVSSIQALEGIEHVRKRPGMYVGGTDIKALHHLVYEVV
                     DNAIDEALAGFCTRIDVTIHKDSSVSVKDNGRGIPVGPHPSKKDANGKPMETVDVVMT
                     VIGAGGKFGGGGYKVSGGLHGVGISAVNALSEWTTTEIKRDGKLWKQEYKRGVPQGKI
                     QQLGKVDKDDTGTTQTFKFDKQIFTEDIDFRFDTLVQRFREMSFVTRGITLKFVDERA
                     DREMTFHFEGGITSFVRYLNRNRENLHSVVHVEKEVENIGIEAAIQYTDAYTESVYSF
                     ANTINTIDGGTHLTGLRSSLTRVINDYARKSGLLKDADPNFSGDDTREGLTAIVSVKH
                     PGPQFESQTKVKLMNPEVQTYVAQVVGETFMTFLEENPQAAKAIIAKCLTSARARDAA
                     RKARDLVIRKSALESLTLPGKLADCSERDTSKTELYIVEGDSAGGSAKQGRDRHFQAI
                     LPLRGKILNTERARLDKILGNNEVKALISALGTGIGDNFDLEGLRYGRVIIMTDADVD
                     GSHIRTLLLTFFFRYMPQLIEDGHLYIAQPPLYRIAHKNNVKYVYSDKEKDKALKELG
                     EKASLQRYKGLGEMNPDQLWDTTMNPSNRTLLLVTVDDAAEADRTFDMLMGDAVDPRK
                     KFIQTHAKSVRNLDI"
     gene            complement(2433..2987)
                     /locus_tag="PKI33_02130"
     CDS             complement(2433..2987)
                     /locus_tag="PKI33_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM35827.1"
                     /translation="MISSDELNPLILSAFTKQITWLEASQKYSLDEFIDTFLNTRAHT
                     LELLDGLTDAQAAFASTVHPFWSISESITHLVYSQGFYHNQLLDIATSQLPHVVEAAR
                     GFGEGAQQHVSVQDLKVSLNAATERIHTVIDSTRNSHDPNKTDASSAFGLCTYKTWIL
                     LLLAHEVDHLRQIAAMRTVAKTNA"
     gene            3093..4034
                     /locus_tag="PKI33_02135"
     CDS             3093..4034
                     /locus_tag="PKI33_02135"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM35828.1"
                     /translation="MKLTTNNIIIIFGSLILCMLALIPITVLAMPLLETNQPDTQATM
                     QAMVTQTVIALTLNAPTQTPLPPTATPVPATNTPVPTVTTLPTVSYCDWMAFVTDISV
                     PDGTNFSAGETFVKTWRIKNRGTCTWTPDYTLVFNGGDQLGSTTSVRLPGYVSPGQYI
                     DVSITFTAPNNPGSYRGYWIMRNTSGALFGTGDKANAPIYVDIRVRQSDPLPHGTISG
                     NLCYPSEFNPRMTLYIENANTGERIQFAITENQYVYEVLVPAGRYYAYAWAPSYSLEG
                     AYAHDNGLMKSFFVESGKETPFINLCNWGPYGHAKGE"
     gene            complement(4086..5273)
                     /locus_tag="PKI33_02140"
     CDS             complement(4086..5273)
                     /locus_tag="PKI33_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNM35829.1"
                     /translation="MHPDFVKPRYDSGGFAGTPKRILDAFASKQYDAVVVFFIDAFGW
                     RFFEKFQDHSFLRRIAKHGKVEKITSQFPSTTAAHVTTMHTGIPVGQSGVHEWYYYEP
                     AVDRIIAPLLFSYATGSKDRDTIDKKIQPLDIFPRGVFYPALKEMGVEAFNYGLRDFT
                     PSTYSKTVMQGSEIISFKTLSEAFINVKVRLEKQTSPTYIHLYFDKIDAVAHEYGPNS
                     AQAEAEIETFLLMMENYFDQIFSSKKRVLFLMTADHGMCEVDPKTTVYLNTHKDFSGV
                     EKFIRSNKRGHLIVPAGSARDMFMYIKNDMVDEAQDFFAKRLEGKADVVKTETLISEG
                     YFGTEISQRFLERVGNVVILPYRYESVWWYEKDKFEQRFRGHHGGLTPQEMETVLYSM
                     EIG"
BASE COUNT         1355 a         1276 c         1369 g         1466 t
ORIGIN      
        1 tcttcatcat tttggtggcg gcacagctta gtgttgtgtt ggcaaatttg gtggttctgt
       61 tcattgcgtt gggttctgta tgggtgattg tgccgatctt tttctgggca catggtgtgt
      121 ttctcaaacg ccagaacgtg ttgacctcca ttctaagcag tttccaactg acacgtttta
      181 ctcttcctac aagcagtctc tttgtgttga cggtttttct gctttcgtat ggattgaatt
      241 atctttggcg tctgccttct ttgaattcgt ggttaacctt gctgggaatc ttcggtcact
      301 catttgtgac cacggcattg ctggcaggta gttttattta ttactatgaa atgagcgcat
      361 gggtgcaggc agttttagag aagctgcgcc cgaataacgt tattaaacaa gcatgatgat
      421 gtgtaagttg tcattgcagg tatttggagg attttgtggc tgaaaacaat tatgatgtaa
      481 gttcgatcca agcgctggaa ggtattgaac acgttcgtaa acgccccggt atgtacgtgg
      541 gtggtacgga tattaaggcg ttgcatcacc tcgtttatga agtggtggat aatgccatcg
      601 atgaagcgct ggctggtttt tgtacacgca ttgacgtgac cattcataaa gatagcagcg
      661 tctccgtcaa agataatgga cgtggtattc cggtgggtcc gcatccttcc aagaaagatg
      721 ccaacggcaa acccatggaa accgtggatg tggtgatgac cgtgatcggc gccggtggta
      781 aattcggcgg cggcggatat aaagtttctg gtggtttgca tggtgtgggt atcagtgcgg
      841 ttaacgctct ttctgagtgg acaaccactg aaatcaagcg tgatggaaaa ctttggaaac
      901 aggaatacaa acgcggtgtg ccacagggca agattcaaca gctcggcaaa gtggataaag
      961 atgacaccgg caccacccaa acgtttaaat ttgataagca gatcttcact gaagatatag
     1021 acttccgctt tgatacgctc gttcagcgct tccgcgaaat gtcctttgtg acgcgcggca
     1081 tcaccctcaa gtttgtggat gaacgcgctg accgtgaaat gaccttccac tttgaaggcg
     1141 gtattacttc attcgtccgc tatttgaacc gcaaccgcga aaacttgcac tctgtggtgc
     1201 atgttgaaaa ggaagtggaa aatattggca ttgaagctgc catccaatat accgatgctt
     1261 atactgagtc tgtgtactct tttgcgaaca ccatcaacac tattgacggt ggtacacacc
     1321 tcactggttt gcgctcttcc ctaactcgtg tgatcaatga ctatgctcgt aagagcggtt
     1381 tgctcaaaga tgcggacccg aacttctctg gtgatgatac ccgtgaaggt ctcactgcta
     1441 ttgtgtcggt caaacatcct ggtccgcagt ttgaatcgca gaccaaagtg aagttgatga
     1501 accccgaagt gcagacctat gtggctcagg tcgtcggcga aacctttatg accttccttg
     1561 aggaaaaccc gcaggctgcc aaggctatca ttgcgaagtg tcttacctcg gcgcgtgcgc
     1621 gtgacgctgc ccgcaaggcg cgtgacctcg tcatccgtaa atctgcgctt gagtcgctga
     1681 ccctgcccgg taaactggca gactgctctg aacgcgatac atcgaaaact gaactctaca
     1741 ttgtagaagg tgactcggca ggtggctctg ctaaacaggg acgtgaccgc cacttccagg
     1801 cgatccttcc gttgcgcggt aagatcctca acaccgaacg tgcccgcctc gataagattc
     1861 tcggtaacaa cgaagtcaag gcgcttatct ctgcgctggg aactggcatt ggcgataact
     1921 tcgacctcga aggtctgcgc tacggacgcg tcatcatcat gaccgatgct gacgtagacg
     1981 gtagtcatat ccgtacactt ctgttgacct tcttcttccg ctatatgccg cagttgatcg
     2041 aagacggtca cttgtatatt gcccagccgc ctctttatcg catcgctcac aagaataacg
     2101 ttaagtatgt gtatagcgac aaagaaaaag acaaggcgct caaagaactc ggcgagaaag
     2161 ccagccttca gcgttataaa ggtctcggtg aaatgaaccc agaccagtta tgggatacga
     2221 ccatgaatcc atcgaaccgc acgttgttgt tggtgacggt ggatgatgct gctgaagcgg
     2281 accgtacctt tgacatgctg atgggtgacg ccgtggaccc acgcaagaag ttcatccaaa
     2341 cccatgccaa gagcgtgaga aatttggata tttagtttag ttgaataaaa aaaacagccc
     2401 gcgaattcgc gggctgtttt ttttattcat gtttaggcgt ttgtctttgc tactgttctc
     2461 atcgccgcga tctgacggag atgatccact tcatgtgcca gcaaaagcag aatccatgtt
     2521 ttgtaagtgc ataacccaaa tgccgagctg gcatctgttt tattggggtc atgtgaattg
     2581 cgagtcgaat cgatcaccgt gtggatccgc tcggtagcgg cattcaggct cacctttaag
     2641 tcttgaaccg aaacatgttg ttgggcgcct tctccaaatc cacgggcggc ttctacaaca
     2701 tgcggcaact gactggtggc aatatccagc aattggttgt gataaaaacc ctgcgagtac
     2761 accaagtgtg taatggattc gctgatgctc cagaaagggt gaacggtaga tgcaaaagcc
     2821 gcttgcgcgt cggttagccc atcgagcaac tccaatgtat gggcgcgggt gttgaggaag
     2881 gtatcgataa attcatccaa agaatatttt tgagaggctt ccagccaagt gatttgcttg
     2941 gtaaatgcgg aaagaataag agggtttaat tcatcggagg agatcatagg ttggatttta
     3001 tcttattttt tttagtactt tggtactgga aatgatggga acaatttgtg ccgggcaaac
     3061 gttctaggtg ggcaaatgaa aaagaggact ttatgaaact tacgaccaat aacatcatta
     3121 ttattttcgg tagcctgatc ttatgtatgc tggcgctgat tccgattacc gtgcttgcca
     3181 tgcctttgtt agagacaaac caaccagaca cgcaagccac gatgcaggcg atggtgaccc
     3241 aaaccgtgat tgccttaacg cttaacgccc cgacacaaac cccgcttcct cctacggcga
     3301 cgcctgttcc tgccacgaat acacccgtgc ctacggtgac aacccttcct accgtcagct
     3361 actgtgactg gatggcattc gtcacagaca tctctgtacc cgacgggacg aatttttctg
     3421 ccggcgagac ctttgtcaaa acatggcgca tcaagaatcg cggcacatgc acttggacgc
     3481 cagattacac ccttgtgttc aacggcggtg accagttagg ttccacaact tcggtgcgtt
     3541 tgcctgggta tgtatctccg gggcaataca ttgatgtgtc cattacattc acagcgccaa
     3601 ataaccccgg ctcgtatcgc ggatattgga tcatgcgcaa cacatctggc gcgctcttcg
     3661 gcaccggtga taaagccaat gcccccatct atgtggacat tcgtgtgcgt cagagtgacc
     3721 cgctgcctca tggcactatt agtggaaacc tatgttaccc gagtgagttt aatccgcgca
     3781 tgacgcttta catcgagaat gccaatacag gcgagcgcat tcagttcgcg attactgaga
     3841 accagtatgt gtacgaagtg ctagtgcctg ccggacgcta ctacgcttat gcttgggctc
     3901 cgagctatag cttggaagga gcatatgctc acgataatgg gctgatgaaa tccttctttg
     3961 tggaaagcgg caaggaaaca ccgtttatca acctgtgtaa ctggggtccg tacggacacg
     4021 cgaaaggcga ataaaaaatc aaaaaagcga cctgtaatag gtcgcttttt tgattgagta
     4081 tattgttatc caatttccat ggaatacaag accgtttcca tctcttgcgg agttagcccg
     4141 ccgtggtgcc cacgaaagcg ctgctcaaat ttatctttct cataccacca tactgattca
     4201 tagcgatacg gcaaaatgac cacattccca acccgctcta aaaatcgctg agagatctct
     4261 gtcccaaagt acccttcaga gatgagggtt tcggtcttga ccacatcggc tttgccttcc
     4321 aaacgctttg caaaaaagtc ttgagcttca tctaccatat cgttcttgat atacatgaac
     4381 atatcgcgtg cagaccctgc cgggacgatc aaatgtccgc gtttgtttga gcggataaac
     4441 ttttcgacac cagagaaatc tttgtgagtg ttgagataca ccgtggtttt aggatccact
     4501 tcacacatgc catgatcggc agtcatcaaa aaaaggacgc gtttcttgct gctgaatatt
     4561 tggtcgaagt aattctccat catcagcagg aaggtttcga tctcggcttc agcttgagcg
     4621 gagttgggtc cgtattcatg agcgacggcg tcaatcttgt caaaataaag atggatgtat
     4681 gtgggggagg tctgtttttc gagccgtacc ttaacgttga tgaatgcctc agaaagagtc
     4741 ttgaaagaaa taatttctga gccttgcatc acggttttgg aatatgtcga gggtgtgaaa
     4801 tcacgcaagc cataattgaa ggcttccacg cccatttctt ttaacgccgg gtaaaacaca
     4861 cctcttggaa agatatccaa tggctgtatc tttttatcga tggtgtcgcg gtctttagaa
     4921 cctgtggcgt aggaaaacaa aagcggcgca ataatgcggt ctacagcggg ttcgtagtaa
     4981 taccattcgt ggactccgct ttgcccgact ggaatgccgg tatgcatggt ggtgacatgt
     5041 gctgccgtag tggatgggaa ttgagaggtt atcttttcca ctttgccatg tttggcgatt
     5101 cgtcgcagaa aactgtggtc ttgaaatttt tcgaagaagc gccagccaaa ggcatcaatg
     5161 aagaacacca caaccgcatc atattgtttg gaggcgaagg catccaaaat gcgtttcggg
     5221 gttccggcga atccgccaga gtcgtatcgg ggttttacaa agtctgggtg catgctttac
     5281 tagaacctgt ataaaaacac tgtgtcaccc tgagggagcg ttcttcgcga ccgaagggtc
     5341 tcttactcca gaggtagaga tccttcgctg tcgctcagga tgacattttt gagacaggtt
     5401 ctatttactg ctttttactt gaccattccc atggctttgc ccgcctgata gatcacatcc
     5461 atggcg
//
LOCUS       DAOREF010000065         5460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8239_length_5460_cov_5.221277, whole genome shotgun sequence.
ACCESSION   DAOREF010000065 DAOREF010000000
VERSION     DAOREF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_5460_cov_5.221277"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="PKI33_02145"
     CDS             complement(<1..1372)
                     /locus_tag="PKI33_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNM35830.1"
                     /translation="MSWSYGEVLKPETINYRRLRPEKDGLFCEAIFGPTRDWQCYCGK
                     YKNPRYKGITCEKCGVEVTRSSVRRERMGHIGLASPVAHIWYTRRIPSQMGMLLDISR
                     RNLDRVLYFAQYIVTYVDEEARKKALQRLEDEITVSEREQASEINNRIIEIKQKRDAS
                     ISELKQKQATLEQGYDEGIAEQLDPVIKEGQKLERLLQDQMGEAAKKAIVFEQTGEKI
                     IDAGDKVASKHITLIQKTVQKKLESLENELKDKRQAEIDDLKTKAATIKAQADTEMEA
                     LRNQLESQTSVSSNNSSRLRDELLELRPFTFLSEIRYRELKQRWGQVFRADMGAEAFY
                     DVLERLDLDKLSEELWHEVKTTKSKQKRKKATTRLKVVEAFKRSGNRPEWMILTVLPV
                     IPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGAPDVIIRNEKRMLQEA
                     VDSLIDN"
     gene            complement(1677..2387)
                     /locus_tag="PKI33_02150"
     CDS             complement(1677..2387)
                     /locus_tag="PKI33_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35831.1"
                     /translation="MNTMNCPNCGAALSAAALKADIATCEFCGTSFRLPKTLTPEPDM
                     GDLLLGADFSQKIMPGWETVNDDKLHFHKGPPAELRGKFTPQVNSYYMLKSSGFLDNF
                     DVSSTLRFFDGDKEWIRAGYFFRHTPEGGYGLMVSAQGSYTFGYVAKDEKNELLFYKI
                     MPWSWHTALRSGMNVFNRLRVICNGSSFRIYLNGVLATSFQDERFKMGRIYLAAEPSE
                     KSSMELGFSDLQVREVPK"
     gene            complement(2384..>5460)
                     /locus_tag="PKI33_02155"
     CDS             complement(2384..>5460)
                     /locus_tag="PKI33_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNM35832.1"
                     /translation="TAASRKPTGGTTPLLNLPNLDENNMPLPNRVEETDLDGTRVTNA
                     AYRPAQDTKPPRPSQGQTNQRPTYRLPQNQNQQPPQYQQPQQYSQYQYQQPAPTGPSF
                     MDRLGAFFGGIGSALRGNKGCLTRGLIIAAFAVVIIGLCGSSILVYQYYSIASTLPDV
                     ADLKNRSSQFQTTRILDRNGQVLYELNDPTAGRRNYIPLSEISPNLVAATIATEDKDF
                     YDNPGFDPIAIIRALWANYITQGEGGGASTITQQLARALLLSPEERTQRTVSRKTREI
                     ILAAEITRRYSKDEILELYLNEIYYGNLAYGIEAAANTYFNKSARDLTIGEAAFLAGL
                     PQSPAVYDIYTNPDVTLIRQQQVLVLMFELSQQQGCIEVSNSTERVCVDPVAATDAAN
                     QIKAYNFIPPAFGAARYPHWVNFIRSELEKLYDAQTIYRSGFVVYTTLDPVLQDRAQE
                     LVTNQVAAMVNNNTKNGALVAIQPSTGQILAMVGSPDFNNAAISGQINMAISPTRQPG
                     SSIKPITYVAAFEKGWTPATWIWDVPTQFPDGANAPYEPRNYDGTFHGGLTVRLALAN
                     SFNIPAVKALEYVGIYDDPNTPEKDGMIAMAERLGITSLTRTDYGLSLTLGGGDVSLF
                     DMTSAYAVFANGGKKLPPVAILKITTFDGEVVYEYQQPEGEQILRPEHAYLISSILSD
                     NEARSYTFGRNSALNLSFPVAAKTGTTNDIRDNWTMGYNPDLVTGVWVGNADYTPMVN
                     SSGLSGAAPIWSQFMEFAVPYLTNGAPTPFSRPSGIVDKVVCRLGGTEPTNECGGEYT
                     EVFASDQLPPTKDQGLVRRVNLDVWTGYEASDACDGPTDEKLVMNVKDPWALKWFDTG
                     DGKAWLTDHGLDENLDIVPDRKCEEGDPQPEIEINLSDGQVISAPVVEVKGTASASGN
                     FKQWRLEFGIGEDPGSWTTLAESNNPVDGGTFTTWDVSNLPSGIVTLRLTLVGERTDI
                     SKRVRLNLSLPIPTVPSATPTLTATPTETPTLIIVIPPSETPTETPTLTPTP"
BASE COUNT         1286 a         1310 c         1484 g         1380 t
ORIGIN      
        1 tgttgtcaat caaagagtca acagcttctt gaagcatacg cttctcgttg cggataatga
       61 catctggcgc gccgagttcg agcaatctct tcaagcggtt gttgcggttg atcacgcggc
      121 gatagagatc gttgagatcg gaagtcgcaa agcgaccacc atccaactgc accatcgggc
      181 gcaggtcggg agggatcacg ggcaatacag tgaggatcat ccactcaggg cggttgccag
      241 agcgtttgaa cgcctccaca accttgagac gggtggtagc tttcttacgc ttctgcttgc
      301 tcttggtggt ctttacttcg tgccagagtt cttcagagag cttatcgagg tcgaggcgtt
      361 cgagaacgtc gtagaaggct tcagcaccca tgtcggcgcg gaagacctga ccccaacgct
      421 gctttaattc acgatagcga atttcgctca agaacgtgaa cggacgcagt tcaagcaatt
      481 catcacgcaa gcgagaagaa ttgttcgaag agaccgaagt ctgcgattcc aactggttgc
      541 gcaaagcttc catctcagta tcagcctgcg ctttgatggt agcagccttg gtcttgagat
      601 cgtcgatctc agcctgacgc ttatccttca actcgttctc aagcgattca agtttcttct
      661 gaactgtctt ctggatcaag gtaatgtgct tgcttgcaac cttgtcccca gcgtcgatga
      721 tcttttcacc ggtctgttcg aagacaatag cctttttcgc agcttcaccc atctggtcct
      781 gaaggaggcg ttcgagtttc tgaccttctt tgatgaccgg gtcaagctgt tcagcaatac
      841 cttcatcgta accctgttca agggtcgctt gcttttgctt cagttcacta atgctggcat
      901 cacgtttttg tttgatctcg atgatgcgat tattgatttc tgaagcctgt tcgcgttcag
      961 aaacggtgat ctcatcttca aggcgctgga gggctttctt gcgggcttct tcgtccacgt
     1021 aggtaacgat atattgagca aagtaaagca cacggtccag attgcggcgg gaaatatcca
     1081 gcaacatacc catctgagat ggaatgcggc gggtatacca aatgtgcgca accggagaag
     1141 ccaacccaat atgacccatg cgctcacggc gcacagaaga acgggttact tcaacaccac
     1201 acttttcaca agtaatacct ttgtaacggg ggttcttgta cttaccgcaa taacactgcc
     1261 aatcgcgggt aggtccaaag atcgcttcgc agaacaagcc gtctttctca gggcgcaagc
     1321 ggcgatagtt aatggtttcg ggtttcaaca cctcgccata agaccaggac ataattgtct
     1381 ggggtgaggc aaggctgata cggagtgctg ttaatccctt ggtttccact ggatcctctc
     1441 caaaaaataa atttcgtttg agcgatacgg tagcgcatag ataacgcgta cctgagacaa
     1501 acaaatgcaa gcgctctgag gtttttcctc aagcgcttgc tggtgttttc tcgtttttta
     1561 catccaactt ggcaattata tggattcagg cactgagagt caaggttaat cagtgaagag
     1621 aggcatactt taaggcggat tcagcgcctc tttcccgtgc caaagcctga ttctgcctat
     1681 tttggcactt ccctcacctg caaatcggag aatcccaact ccatagaaga cttctcgctt
     1741 ggctcagcgg caaggtagat cctacccatt ttgaaacgct catcctgaaa cgacgtggca
     1801 agcacaccgt tcaagtagat gcgaaaactg ctgccattgc atatcacccg caagcggttg
     1861 aagacattca tgccagaacg taacgccgta tgccacgacc atggcatgat cttataaaaa
     1921 agcaattcat ttttttcatc tttggcaaca taaccaaacg tatacgaacc ctgtgccgac
     1981 accatcaacc catacccacc ttcaggagta tggcggaaga agtaccccgc tcgaatccac
     2041 tctttatcgc catcaaaaaa gcgcagggta gaacttacat cgaagttatc taaaaagcca
     2101 gaactcttta gcatgtaata cgagttgacc tgtggcgtga attttccgcg caactcggcg
     2161 ggcggacctt tatggaaatg taacttgtcg tcgttgaccg tttcccaacc aggcatgatt
     2221 ttctggctaa agtctgcgcc gaggagtagg tcgcccatat ctggctcggg ggtgagggtt
     2281 ttcggcaggc gaaaactggt gccgcaaaat tcacaggtgg caatatctgc tttgagggct
     2341 gcggctgata acgccgcgcc acagttgggg caattcatcg tgttcatggt gtaggagtca
     2401 aggtcggcgt ttcggtcggt gtttcactgg gtggaataac gataatcaac gtcggcgttt
     2461 cggtgggcgt tgctgtcagc gtcggcgtgg cagaaggtac ggttggaatg ggcaggctca
     2521 aattaagtct gacgcgttta ctgatgtctg ttcgttctcc gaccagagtc aagcgcaggg
     2581 ttacgattcc gcttggcagg ttggaaacat cccaagtagt aaatgtgccg ccatctactg
     2641 ggttattgct ttctgcgagg gtggtccatg aaccagggtc ttcgccgatg ccgaattcta
     2701 aacgccattg tttgaagtta ccgctggcag aggcagtgcc tttgacttca accacggggg
     2761 cagaaatgac ttgaccatcg gagaggttta tttcgatctc tggttgcggg tcaccttctt
     2821 cacacttgcg gtctggcacg atgtctagat tttcatccaa gccatgatct gttagccagg
     2881 cttttccatc gcctgtatca aaccatttga gcgcccacgg gtctttgacg ttcatgacga
     2941 gcttttcatc ggtgggacca tcacatgcat ccgaggcttc atagccagtc caaacgtcca
     3001 ggtttacgcg gcgtacaagt ccctgatctt ttgtgggagg caattggtct gatgcgaaga
     3061 cttcggtata ttcgccgccg cattcattgg tgggttcggt tccgccgaga cgacagacaa
     3121 ctttatccac gatgccagat ggacgcgaga aaggagtcgg cgcgccgttg gtgagatagg
     3181 gcacggcaaa ttccatgaac tgtgaccaga tcggagcggc tccgctcaag ccggatgaat
     3241 tgaccatagg ggtgtaatca gcatttccaa cccacacgcc tgtgaccaag tctgggttat
     3301 agcccatcgt ccagttatcg cggatgtcgt tggtggtacc ggttttggct gccaccggga
     3361 aggaaagatt caatgccgag ttgcgaccaa aggtgtagct gcgcgcttcg ttatctgaaa
     3421 gaatggagga gatgagatag gcatgttcag gacgaaggat ctgttcgcct tcgggctgtt
     3481 gatattcata aaccacttcc ccgtcaaagg tggtgatctt aagaatggcg actggcggga
     3541 gtttcttgcc gccattggca aagactgcat aggcactggt catatcgaag aggctgacat
     3601 cgccgccgcc caaggtgagg gacagaccat aatcggtacg cgtgagcgat gtgatgccaa
     3661 ggcgttctgc catggcgatc atgccatctt tttcgggggt gttcgggtca tcgtaaatgc
     3721 cgacatattc caatgctttg acggcaggga tgttgaagga gttcgccaag gcgaggcgga
     3781 cggtgagtcc accgtggaag gtgccatcgt agttacgcgg ttcatagggg gcgttggcac
     3841 cgtctggaaa ttgagtgggg acatcccaga tccatgtggc gggggtccaa cctttttcaa
     3901 aggcagccac ataggtgata ggcttgatcg acgaaccagg ttgacgcgta gggctgatcg
     3961 ccatattgat ctgaccggaa atcgccgcgt tattgaaatc tggcgagccg accatggcaa
     4021 gaatctgacc ggtggaaggt tgaatcgcca ccaaggcgcc gttcttggta ttgttgttca
     4081 ccatagcagc aacttggttg gtaaccaatt cctgtgcgcg gtcttgaaga accgggtcga
     4141 gcgtggtgta tacgacaaag ccagagcgat agatggtttg ggcgtcgtat agtttttcca
     4201 attccgaacg gatgaaattc acccaatggg ggtaacgtgc cgcaccaaaa gcaggcggga
     4261 tgaagttgta tgccttgatc tgattggcag catcggttgc tgcaacgggg tccacacaga
     4321 cacgttctgt gctattactc acttcaatac aaccttgttg ttggctcaat tcgaacatca
     4381 agaccaaaac ttgttgctga cggatcaagg tcacatcggg gttggtgtaa atatcgtaca
     4441 ccgccggaga ttgcggcaag ccagccagga aggcagcttc accaatggtc aagtcgcggg
     4501 cagatttgtt gaagtaggta ttcgccgccg cttcaatacc gtatgcgaga ttgccataat
     4561 aaatttcgtt cagatacagt tcgagaattt catccttgga gtaacggcga gtgatttctg
     4621 cggcaaggat aatctcgcgt gtcttgcggg agacggtacg ttgcgtgcgc tcttcgggag
     4681 aaagcaaaag ggcacgcgct aattgctgtg taatggttga agcgccaccg ccttcacctt
     4741 gtgtgatgta gttcgcccac aaagcgcgga tgatggcaat agggtcgaag ccggggttat
     4801 cgtaaaaatc tttatcttcg gtagcaatgg tcgctgcaac tagattcgga gaaatctcac
     4861 tcaatggaat atagttgcga cgtccagctg tggggtcatt caattcgtac aagacctgac
     4921 cgttccggtc aaggatacgg gtagtttgaa attgcgatga acgattcttc aagtccgcta
     4981 catcgggcag ggtgctggca atggagtaat actgatagac aaggatcgaa ctgccacaca
     5041 aaccgatgat tacaactgca aaagcggcaa taatcaaacc acgggtgagg cagcctttat
     5101 ttccacgtaa ggcggagcca attccgccaa agaatgcgcc aagacgatcc ataaaagatg
     5161 gacctgttgg cgcaggttgt tggtattgat attgcgaata ttgttgtggc tgctgatatt
     5221 gcggaggttg ttgattctga ttttgcggca aacgataggt tggtcgctga ttggtctgac
     5281 cctggctcgg acgtggcggc tttgtatctt gcgccgggcg atatgccgca ttggttaccc
     5341 gcgtgccatc gagatcggtc tcttcgacgc gattggggag cggcatgtta ttttcgtcga
     5401 ggtttggcag attcagtagc ggcgttgtcc cacctgtcgg tttgcgagaa gcagctgtcc
//
LOCUS       DAOREF010000066         5366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8468_length_5366_cov_4.652231, whole genome shotgun sequence.
ACCESSION   DAOREF010000066 DAOREF010000000
VERSION     DAOREF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_5366_cov_4.652231"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKI33_02160"
     CDS             complement(<1..253)
                     /locus_tag="PKI33_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM35833.1"
                     /translation="MNNPPRILIVDDEPFNVDYLEQELADFGYELLTAANGEEALEKI
                     RTGAPDLVLLDIMMPIMDGFTVLEQAKADAAIRNIPIIII"
     gene            complement(265..618)
                     /locus_tag="PKI33_02165"
     CDS             complement(265..618)
                     /locus_tag="PKI33_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM35834.1"
                     /translation="MKTILIVEDTDLNIDLLTQLLEDDYKLLIAKDGNQGVNMAISKK
                     PDLILMDISLPILDGYEATRQIRKSLPHTPIIGLSAHAMDGHRQKAQDAGCNDYLTKP
                     VNDSLLFDMLAKYLG"
     gene            complement(631..>5366)
                     /locus_tag="PKI33_02170"
     CDS             complement(631..>5366)
                     /locus_tag="PKI33_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM35835.1"
                     /translation="ENARLFDETQRLLKITEDRAAELAIINSVQEGLASKLDMQAIYD
                     LVGDKLAEVMNTLDIDIRIFSPETNQVFFPYLREHGERLEISPSPMRGFAKKIHETRQ
                     TIVINENLAERMAELGSVLVPGTQMEKSFMAVPILVGGAALGMVSISNYEKENAFGDS
                     DIRLLQTVVSAMSVALENARLFDETQRLLKITEDRATELSVINSIQQGLAAELDPQAI
                     IELTGDKLRETLQTEDLGIRLYDAQNNLIHYVYEYIQGERREYPSKPPVPGGTFERIF
                     KTRQPFIRNTLAEREALGLALLPNTEAAKSSVNVPIIGSDRVIGVIIVQSHEKEYAFS
                     DAQVRLLQTVAASMGVSLENGRLFDETQRLLKETEQRNAELAIINAVQQALVSNLDMK
                     SIYQAVGRKLTEIFSVQSAAIYTVDFNTKVMTYEFAYEQGKEWEIEPKPATGLHLNVI
                     NQVLSSRKSFVVNTNFNEYASQFADFQSSRSRNPKSLCAVPILIRENSITGISLQHLE
                     KENFFDESALRLLETISSAAGVALENARLFDETQRLLQETEERAAELGAISTVTQALV
                     AETELDNMIQLIGSQMRDTFNADIAYLALLDQQTGIIQFPYQYGDDIPPTPIGEGMTS
                     RIIRDGKPLIFNRNIEEQSSVLGIQRQGRKARSYLGVPIKAGRETIGALSVQSTQKEG
                     VFDEDSLRLLSTVAANAGAAIKTARLHAETQRRAREMSTLAEVGRDISSSLEATTVLE
                     GIANHARELLSGDLSALFLPEQNGKIFRAIAAVGEEAEELRNDTILLGEGLLGSIALT
                     KKAEIVNDTNNDPRALMITGTESLSDEHMLAIPLLANSELKGLMAVWRHGADNLFTEA
                     ELEFMNNLARQAVIAIQNTQLFEQTQHLLNQTEQRAVELAILNSVSESMTRTLDVSTV
                     THNVGNKIHEIFNAEIVDILLYDAATNIVQLAYSFSDNIHYEDEPPWELGEGLTSKII
                     ISKQPLLLNTSQEINDHGAAAYLTAPEDEEEIKSYMGVPIMVGDQVLGVVDVQSYKAG
                     AFNEDNLRILQTLSANMGVAIQNARLFNETQRLFKEAQEARQAAEHANQAKSAFLANM
                     SHELRTPLNAIIGFTRIVRRKSEGLLPDKQTENLDKVLSSSEHLLSLINTVLDIAKIE
                     AGRMDVQAANFNVSSLMDLCANTATPLMKPTVRLVKDVEEDLGMIYSDQDKIKQIVLN
                     LLSNAAKFTAAGKITLDAHRAEDLLVVNVSDTGIGITEEALGRIFEEFQQADTSTTRQ
                     YGGTGLGLTISRNLARLLGGDLTATSEHGKGSTFTLTLPIQYGIKPASSLNPDTAPLH
                     RSGTSTKADASKKLILVIDDDPDATYLLRENLGPEEFEIIGARNGIIGQEKARELKPD
                     AILLDILMPEKDGWQVLHDLKNDPATTNIPIILLTIVDKKALGMRLGAAAYLLKPLNP
                     AEVLSTLQKVIKPKENARIHVLTVDDDPHIAEMLSQQLPESEFRLESALDGEAGLAAI
                     EAEHPDVILLDIMMPKLDGFGVIERLREKPETRELPIIVISAKELTDDEIARLRESVV
                     LIMRKQGFDGEKLAQEIKHTVQHAN"
BASE COUNT         1229 a         1276 c         1373 g         1488 t
ORIGIN      
        1 aaataataat gattgggata ttacgaatcg ccgcatctgc tttggcttgc tctaataccg
       61 taaacccatc catgatgggc atcataatat ctagtagtac aaggtctgga gcgccagtac
      121 gaattttctc cagtgcctcc tctccgtttg cagcggtaag aagctcatac ccaaaatcag
      181 caagttcctg ctctaggtaa tcaacattga atggttcatc atctacgatc aaaatacgcg
      241 gtggattgtt cataaaagaa agacttatcc caagtatttg gcgagcatgt caaacagtaa
      301 agaatcattc acgggttttg tgaggtaatc gttacagcct gcgtcttgcg ctttttgtcg
      361 gtgcccatcc atggcgtggg ctgaaagacc aatgattggc gtgtgcggaa gcgacttccg
      421 tatctgacgt gtagcttcat acccatccag aatcggcagc gaaatatcca tcaggatcaa
      481 atcaggcttt ttgcttattg ccatgttcac accctgatta ccatctttag cgattaacaa
      541 tttgtaatca tcctcaagta attgggtgag taggtcaatg ttcaggtctg tatcttcaac
      601 gatcaatata gttttcatac actctccaat ttaatttgcg tgttgcacag tgtgtttgat
      661 ttcttgtgcc aatttttcac catcaaagcc ttgcttgcgc atgataagaa ctacactctc
      721 gcgcaatctt gcaatttcat catcggtcaa ctctttggcg ctaatgacga tgataggcag
      781 ttcacgcgtc tcaggctttt cgcgcaagcg ttcgatgacg ccaaaaccat ccaacttggg
      841 catcataata tcaagcaaaa tgacatctgg atgctccgcc tcaatcgccg ccaaacctgc
      901 ttcaccatcc aaagcggact ctagtctgaa ttcggattct ggtaactgct ggcttaacat
      961 ctcagcaata tgtgggtcat catccaccgt gagaacatga atacgagcat tctctttagg
     1021 tttgatcacc ttttgcaacg tggataacac ttctgcgggg ttgagcggtt tgagcaaata
     1081 ggcagcggcg cccaagcgca tgcctagcgc ctttttatcc acaattgtaa gcagaatgat
     1141 tggaatatta gtcgttgctg ggtcgttttt caggtcgtgc aaaacttgcc agccgtcttt
     1201 ctctggcatg agaatatcga gcaaaatggc atctggcttt agctcgcggg ctttttcctg
     1261 cccgatgatg ccgttgcgag cgccgatgat ctcgaattct tcgggtccga gattttcgcg
     1321 caaaagataa gtagcatcgg ggtcatcgtc gatcacgaga atgagctttt tggaagcatc
     1381 agctttggtt gaggtgccag atcgatggag gggagccgta tcgggattaa gtgacgaggc
     1441 gggttttatc ccatattgaa tggggagggt gagggtgaat gttgaacctt ttccatgttc
     1501 actggtggct gttagatcgc cgcccaagag gcgtgccaga ttgcgactga tggtgagacc
     1561 caacccggtg ccgccatatt ggcgggtggt gctggtatcc gcttgctgga attcttcgaa
     1621 gattctgcct agggcttctt cggtgatacc aatacccgta tcgctgacgt tgacgaccaa
     1681 taaatcttca gcgcggtgcg catctaatgt aatcttgccc gccgcagtga acttggcagc
     1741 attgctcaat aagttgagca cgatctgttt gattttatct tggtcagaat agatcatgcc
     1801 gagatcttct tctacatctt ttaccaaacg aaccgtaggc ttcatgagcg gagttgccgt
     1861 attggcacac aggtccatca gcgaactgac gttgaagttt gccgcctgca cgtccatgcg
     1921 cccggcttcg atcttggcaa tatcgagcac ggtgttgatg aggctaagca aatgctcaga
     1981 actgcttaga accttatcaa gattttcggt ttgtttatct ggcaataagc cttcgctctt
     2041 tcttctcaca atgcgtgtga agccaatgat ggcattgagc ggcgtgcgta actcatggct
     2101 catattggca aggaaggcgc tctttgcctg gttggcatgt tctgctgcct gacgggcttc
     2161 ttgagcttct ttgaaaagac gctgagtttc attgaagagg cgggcatttt gaatggcaac
     2221 gcccatgtta gcggagagag tttgcagaat gcgcaaatta tcttcgttga aggcgccggc
     2281 tttatagctt tgaacatcca ccacgcctag tacctgatcg ccgaccataa tgggcacacc
     2341 catatacgac ttgatttctt cttcatcttc aggggcggtg agataggctg ccgcaccatg
     2401 gtcattgatc tcttgcgatg tattcaagag caaaggctgt ttcgagatga tgatcttgga
     2461 tgtaagccct tcacccaatt cccaaggtgg ttcatcttcg tagtggatat tgtcgctaaa
     2521 gctgtatgcc aattgaacaa tattcgtagc ggcatcgtag agcagaatat ctacgatctc
     2581 ggcgttgaag atctcatgga tcttgttacc gacattgtgc gtcacggtgc tgacatccaa
     2641 tgtgcgcgtc atggattcac tgacgctgtt aagaatagca agttccacgg cgcgttgctc
     2701 ggtttgattg agcaggtgtt gagtttgctc aaacaactgg gtgttctgaa ttgcgatcac
     2761 tgcctgacgc gcaaggttgt tcatgaattc cagttcagct tcagtgaaga ggttatcagc
     2821 tccatgacgc caaactgcca tgagaccttt tagctcagaa ttagcaagca atggaatcgc
     2881 aagcatatgc tcatctgata aagactctgt tccagtgatc atcaatgcgc gtgggtcatt
     2941 gttggtgtcg ttaacgatct ccgctttttt cgtgagcgca atactgccga gcaacccttc
     3001 cccaagtaag atggtgtcgt ttctgagttc ttcggcttct tcgcccactg ccgcaatggc
     3061 acggaaaatc ttcccatttt gttctggcag gaagagcgca ctgaggtcac cacttagcaa
     3121 ctctctggca tgatttgcaa tgccttcaag caccgtagta gcttcgaggc tggatgaaat
     3181 atcgcgccca acttctgcca gcgtagacat ttctcttgca cggcgttgcg tctcagcatg
     3241 taagcgcgcg gttttgatgg cggcacctgc attggcagca acggttgaaa gcaaacgcag
     3301 cgagtcttcg tcgaatacgc cttccttctg cgtgctttga acactgagcg caccgatcgt
     3361 ttcgcgaccg gctttaatcg gcacgcccaa ataagaccgc gcttttcgtc cctggcgttg
     3421 aatgccaagc accgagcttt gctcttcaat gttacggttg aagatcaatg gtttcccatc
     3481 acggatgatg cggctggtca tgccttcgcc aattggggtg ggtgggatgt catccccata
     3541 ttgataaggg aattggataa tgcctgtctg ttgatcgagc aatgccagat aagcaatatc
     3601 ggcattgaac gtatcccgca tctgactccc gatgagctgg atcatgttat ccagttctgt
     3661 ctctgccacc aaagcctgag tgacggtact gatggcgccc agctcagcgg cgcgttcttc
     3721 ggtctcctgt aagaggcgct gggtttcatc aaagagacgg gcattttcga gcgcaacccc
     3781 agccgcactt gagatcgtct ctagcaatct cagtgcagac tcgtcgaaga agttctcttt
     3841 ttcaagatgt tgcagtgaga tgcctgtaat cgaattctca cggatcaaga tcggcaccgc
     3901 acaaagtgat ttgggattgc gtgagcgcga tgactggaaa tcagcgaact gcgaggcata
     3961 ctcattgaag ttggtattca caacaaagct cttgcgagag gaaagcactt ggttgatgac
     4021 attcaaatgc agacccgtag ctggtttggg ttcgatctcc cattctttgc cttgttcata
     4081 ggcgaactca taggtcatga cctttgtgtt gaaatcaaca gtatagatcg ccgcagattg
     4141 gacactgaag atttcggtta actttctgcc cactgcctga taaatggact tcatgtccag
     4201 atttgagacc aaggcttgtt gaacggcatt gatgattgcc aactcggcat tgcgttgttc
     4261 ggtttctttc agcaagcgtt gagtttcgtc gaagagacgt ccgttctcaa gcgatacacc
     4321 catgctagcg gcaacggtct gcaacaaacg cacctgcgca tcgctgaagg cgtattcttt
     4381 ttcgtggctt tgtacgatga tgacaccaat gacacggtca ctaccgatga tgggcacgtt
     4441 gacgctggat ttggcggctt cagtatttgg caacaaggca agtccgagtg cttcacgttc
     4501 agccagagta ttgcgtataa agggttggcg ggttttgaag attctctcga aggtgccgcc
     4561 tggcactggt ggtttggatg ggtactcgcg gcgttcaccc tgaatgtatt catacacgta
     4621 atgaatgaga ttgttttggg cgtcgtacaa gcgaatgccg aggtcttcgg tttgaagtgt
     4681 ttcgcgcagt ttgtctccgg tcaactcgat aatggcttgc gggtctaatt cggctgccaa
     4741 gccttgttga atgctgttga tcacggatag ttctgtggcg cggtcttcgg tgatctttag
     4801 gaggcgctga gtttcatcga agaggcgcgc attttcgagc gccacactca tggcagagac
     4861 cacggtttgg agcaggcgta tatcagaatc gccaaaggca ttttcttttt cgtaattgct
     4921 gatgctgacc atgcctaatg ccgcgccgcc caccagaatg ggcaccgcca tgaaggactt
     4981 ttccatttgc gtgccgggca ccagcaccga gccgagttct gccatgcgct ctgccaggtt
     5041 ttcgttgatt acgattgttt gacgggtttc atgaatcttt ttagcaaacc cgcgcatggg
     5101 cgagggggag atttccagcc gttccccatg ttcacgcaaa taggggaaaa agacttgatt
     5161 cgtctctggc gagaagatgc ggatgtcgat atcgagggtg ttcatcacct cggctaattt
     5221 atcgccgacc agatcgtaaa tggcttgcat atccagtttg gaggcgagcc cttcctgcac
     5281 cgagttgatg atggcaagtt cggcggcgcg gtcttcggtg atcttgagca gacgttgggt
     5341 ctcgtcgaag agacgggcgt tttcga
//
LOCUS       DAOREF010000067         5339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8549_length_5339_cov_3.928463, whole genome shotgun sequence.
ACCESSION   DAOREF010000067 DAOREF010000000
VERSION     DAOREF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8549_length_5339_cov_3.928463"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1232
                     /locus_tag="PKI33_02175"
     CDS             145..>1232
                     /locus_tag="PKI33_02175"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNM35836.1"
                     /translation="MSQSRLDKLTASLRTSDLDAVILNPGPTLKYLTGISFHLMERPV
                     VLFVAPGKDPVLVLPELELLKLDLFPYKVQGVAYGENPAEWDDAFRKAAQALGVDGKR
                     IGVEPRNLRLLEFRHVKAGAPEADFPDASDVLAELRLRKDKAEVDAMRKAVKIAQDAL
                     EATVPQIKIGMTEKEVASELVMQLLKHGSDSELPFPPIVSAGPNAANPHASPSDRKLQ
                     AGDLLVVDWGAAYEGYISDLTRTFAVGEMDAECQKIHKLVQEANAAGRAIAKPGIPCA
                     DVDKATRDVIEKAGYGKYFNHRTGHGIGMEGHEAPYMRGDNMQILEPGMAFTVEPGIY
                     LTNRNGVRIEDNMVITENGAESLSDMPR"
     assembly_gap    1233..1332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1337..2539)
                     /locus_tag="PKI33_02180"
     CDS             complement(1337..2539)
                     /locus_tag="PKI33_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNM35837.1"
                     /translation="MTERNTIYQIKLDGHIGPQWADWFDGLSITQDKGQTLLTGPVPD
                     QPALYGVLKKVRDLGVPLLSVNILELDTFTHIRRFSMKAIVNTQYGSPDVLQLKEVAK
                     PTPQGNEVLIKIHATAVNAADWHLLKADPFLVRLQYGLFNPKYTILGADIAGRVEAVG
                     LNVQKFKVGDEVFGDLSGAGWGGYAEYVSVREDILVHKPANITFEEAAATPMASVTAL
                     LGLKEGGIHQGQKVLINGASGGVGTFAVQIAKAFGAEVTAVCSTRNVELARSLGADHV
                     IDYTKEDFTKSGKQYDLILAANGYHSLAEYQRALTANGKYVMTGGTTAQMFEVLLFGR
                     MKSKGNQKLGNILAKPSQKDLEFVAGLLADGKVKPVIAKRFALAHTADAIRYVEEGHA
                     QGKVVVTI"
     gene            complement(2627..2812)
                     /locus_tag="PKI33_02185"
                     /pseudo
     CDS             complement(2627..2812)
                     /locus_tag="PKI33_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(2933..3949)
                     /locus_tag="PKI33_02190"
                     /pseudo
     CDS             complement(2933..3949)
                     /locus_tag="PKI33_02190"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1275 a         1300 c         1245 g         1419 t
ORIGIN      
        1 tcattacttg gaagaaaagc ggtttttgac taatgtaaaa agatgagagt acaacaatac
       61 tccataagat gaaagttgta actatagcga tttttttgaa cattcttcta agttctccat
      121 acaaaattat aaaggaataa aaccatgtct caatctcgac tcgacaaact caccgcttct
      181 ctgcggacct cagacctgga tgctgttatt cttaatccgg gtcctacgct gaaatatctt
      241 acaggtatta gttttcattt gatggaacgt ccggtagtct tgtttgtggc gcccggcaaa
      301 gacccagtgc ttgtcttgcc tgagcttgaa ctcctcaaat tggacttgtt cccttataag
      361 gtacaaggtg ttgcctatgg tgagaatccc gcagagtggg atgatgcttt cagaaaggct
      421 gctcaagcct tgggcgtgga tgggaagcgg atcggcgtcg aaccgcgcaa tttacgcttg
      481 ctggaatttc gtcatgtcaa agctggtgcc ccagaagcgg atttcccaga tgccagcgat
      541 gtccttgcag agttgcgcct tcgcaaagac aaagccgaag tcgatgccat gcggaaggcg
      601 gtgaaaatcg ctcaagatgc tcttgaggca accgttcctc agatcaaaat tggaatgacc
      661 gagaaagaag ttgcctctga gttggtgatg cagttgttga agcatggttc tgattctgaa
      721 ttgccattcc ctcccattgt ttcagccgga ccaaacgctg ccaacccaca tgcttcgcct
      781 tctgaccgta aacttcaagc cggtgatttg ttggttgttg attggggtgc cgcttacgag
      841 ggttacatct ctgacctgac ccgcactttc gcagtggggg agatggatgc tgaatgtcag
      901 aagatccata agcttgtaca agaagcgaat gccgctggtc gtgcgattgc caaaccgggt
      961 atcccttgtg ctgatgtaga taaggcaacc cgtgatgtca ttgaaaaagc cggttatgga
     1021 aaatatttca accaccgcac gggtcacggc attggtatgg aaggacacga agctccatac
     1081 atgcgaggtg acaatatgca aattttagag ccgggcatgg cgttcacggt tgagccggga
     1141 atttatctca ccaatcgcaa cggcgtacgc attgaagata acatggtcat tactgagaat
     1201 ggtgcagaaa gcctctctga tatgccaaga gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnttttctat atcgtgacaa caactttgcc ctgcgcgtga ccctcttcca
     1381 cgtagcggat agcgtctgcg gtatgggcaa gtgcaaagcg ttttgcaata accggtttca
     1441 ctttgccgtc tgccaacaag cctgctacaa actccaaatc tttttggctg ggttttgcca
     1501 aaatgttgcc cagcttttga ttgcccttcg atttcatacg cccgaatagc agcacttcaa
     1561 acatttgggc agttgtaccg cctgtcatca catatttccc atttgccgtt agagcgcgtt
     1621 gatattcggc aagggagtga tatccatttg cagctaggat caagtcatat tgcttgccgc
     1681 tcttggtgaa atcttctttg gtgtaatcaa tgacatggtc tgcgccaagt gagcgtgcca
     1741 attccacatt acgcgtgctg caaaccgcgg tgacctctgc cccgaaggct ttggcaattt
     1801 gcacggcgaa ggtccctacg ccgcccgatg ctccattgat caaaactttt tgtccctgat
     1861 gaatgccccc ttctttgagt cccagcaagg ctgtgactga tgccattgga gtagctgccg
     1921 cctcttcaaa ggtgatgttg gcgggcttgt gcacaaggat atcttctcgc acagatacat
     1981 attctgcgta accgccccag cctgcgccgg ataagtcacc gaagacttca tcgcccactt
     2041 tgaacttctg cacattcagc cctaccgctt cgacacgccc ggcaatgtct gcgccgagaa
     2101 tggtgtattt gggattgaac aacccatact gtaagcggac gaggaacggg tctgccttta
     2161 acaagtgcca gtctgcggcg ttgacggcgg tggcgtggat tttgatgagt acttcatttc
     2221 cttgcggggt gggttttgca acttccttga gttgcagaac atcgggcgaa ccgtattggg
     2281 tgttgacaat ggctttcatg gaaaatctcc ttatatgggt aaaagtatca agctcaagaa
     2341 tattgacaga cagcaacggt actcccagat cacgcacttt tttcaagact ccgtataggg
     2401 cgggctggtc cggtacgggt ccggtaagaa gcgtttgacc tttatcctgc gtaatggata
     2461 gcccatcaaa ccaatctgcc cattgcggac cgatgtgccc atccagtttg atttggtaga
     2521 tcgtgttgcg ctcagtcatg tctgcctttc gacgcgcgaa actataacgg caggcgaggc
     2581 tggcgtttag atacttaagt attgatttga gtattgtttg ggaaaactag atgagcccta
     2641 gctctcttgc ccgggctacg gcgtctgtgc ggcttttcgc ttggagtttg ttgaagatgc
     2701 gcaaattatg tcctttgact gtgctaagag aaaggaacag tttttcactg atctctgtgt
     2761 tcgaaagccc taacgccacc aaactcaaca cttcaagttc ccgctcactc aacgggtcga
     2821 ttaacagttg agacggagcc ggttttggag tttggacttc aaaaaggctt aacaatgttt
     2881 gtatataatt cttgttagct ttgccttcag gggtaaggct taactttgcc agcaattcct
     2941 gcatgggctt gccttcttca aagatgcgca cgtagccttc tgcttcagcg agagccacag
     3001 cccgctttag cgggaggagt gcttgtgtca actgtccctg cgcgtgaaac gcaagtgctt
     3061 gtaagatcaa gatctcgagc atacttccaa tgcgtgcgcc atcctcagct gcctgtaaga
     3121 gacgcgctag aaactgtgat acgttttttg aatctttttt cgcaagcaac acgcgcgcga
     3181 aggtgatgtg ctcaaactcg tgcaagaaac ttaattcatc gtccattgaa aacttctgtg
     3241 cccactcgtg tgtttgggcg agcaaaccct gcttgagata tacacgaact ttgaatgcat
     3301 ctacagggcg tgttaacgga atcggtgtct tcacataggt gcgtttggct tcgtcaaaaa
     3361 aatacaatgc ggcgtttaaa tcaccggcag tttctttgaa gcgtgccaat gcaaagttgc
     3421 gacgatactg ccaatctatt attgtggcgg cttgccctag ttccaaactt agttggagat
     3481 gtttttctgc ggcatcattg gcgcccatct cgttcaacaa caatgccatc cctaaatgat
     3541 gatgcgctgt aaaaggggca acttccgcgc ctacgtctgc tgcaaatttc aaagcatctt
     3601 catatatttg catggcattg cgtaagcgcc cttgctcttg taagatatct gccaagccag
     3661 atgcagtcat gatcgcaaac atggtattgc ctgccaaccg cgcttgctct gcccaatggt
     3721 tcatcgcgtc atacgcggca gtcaattcgc catttgccca atatgcacct gccaaaattg
     3781 ctgtcgcttg tgcacgcata aattgatttg tttcagggct aagccttact gcttcttgtg
     3841 ccaacttcac cgttgcgaga ctttcccctt ttgtctgtga attgtaggcg cgggcaatgg
     3901 caattcgagc tggcagggtt ttgaactgtt cttcttcaac gacaaccatc gtatgcgctg
     3961 cttcgcgtaa acactgttct gcctcttgta aaaaggattc gctttcatct gctttgctgg
     4021 catccatgta tgcccaagcg atctgcacac acaacacagg acgcaaacgc ctcacactcc
     4081 ctgatagctg tttcacccac ccaagccact gcccagactg aaaggtttcg tccatgccga
     4141 gccaggctcg ctcagctata tctgctgctc ggttttggtc tttagcagcc atggcatgac
     4201 ggaatgcgtc tgccaaatca ccattttgtt caaaccatgc gctggcgttt aagtgcaact
     4261 ctgccacttc gttcgaggca atatgttgct ctgaccgctg ccgcaacaaa tcaccgaaca
     4321 aatggtgata gcgataccaa ttccgttcgc catctaatgg aacgatgaac aaatttgagc
     4381 gttccaattc ttccaacatt ttctggctgg gcataccagt cacagcctca catagcggac
     4441 catttaatcg ttccagaatt gacgtgcgca acaaaaaatg ctgaacttct tgaggctgtt
     4501 tacttaatac ttcctctagt aagtaatcca acacaaaatg atgcgctccg gtaaacgatt
     4561 ggataaaacc tgtgatgtca tcgtgaccct gcatggagag tgctgccaat tgcagacctg
     4621 ccacccaacc ttcagtacga gtttctaatg ctgtaatatc ttgcccagaa agatccaacc
     4681 ccattacctg attgagaaaa tccgcagctt cttgcatggt gaagcgtaaa tctgctgccc
     4741 gcagttcggt caattgcccc ttcgcccgca gacgtgccaa cggcaggggc gggtcttcac
     4801 gtgaagcgat tatcaagtgc atttgtggcg gcatgtactc taccaagaag cgcaaagctt
     4861 catccacagg cttggagtct acttcgtgat aatcatctaa tactaggata aagtcttgtg
     4921 agactgagtc tatgtcattg agtaaggcta ccaataaggt gtctactgca ggtggctgat
     4981 gagatttaag tgcgccaagt acactttcac ctatactctt gtctatgacc tgtaacgctg
     5041 agaccaaata tgtaatgaaa cgcgttatgt catggtcgct atcatccaaa gacagccatg
     5101 caacttttag gtttacttct cccaaccatt cactcaacaa agttgttttt ccaaaacctg
     5161 ccggtgcaga aaccaatgtc agtgcgtggt tcaacccttc ctgcaaccgt tccagcaagc
     5221 gctggcggat cacaaccttt gtccgcagcg gggggacacg taactttgtc gtcataacag
     5281 ggatcggcat gtgggtaatt ataaagtgcc tagctggaat ctccgcaagg gtttagcga
//
LOCUS       DAOREF010000068         5301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8640_length_5301_cov_4.439192, whole genome shotgun sequence.
ACCESSION   DAOREF010000068 DAOREF010000000
VERSION     DAOREF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8640_length_5301_cov_4.439192"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PKI33_02195"
     CDS             <1..1298
                     /locus_tag="PKI33_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35838.1"
                     /translation="FEVLDSHFTWSHFPKQVKIDDADIKQWMISLPLTNLPRITQSIS
                     NLPTSPDEKELEEAIRETITTSWKSLSYSQIDLAVNTFMNCIREALLPLKKQTLLIVG
                     RAVLRTEDKINLLIKLTEKLIEQSSKPSTTNTTNPDKQFSKPDTRQETLYSNLLKIES
                     YAKRIYVAPTHARGRNEIFRNLLKLSGHANSEFILKNKTIISFHDLCSYPWTEVCDSS
                     LAIEYSSAEWALSEKIDKRNEFIDLLNKSIEAKLYPEVLFDREEKYYYFAPTEDLRDK
                     ELSYPSLQKNTSRFVFKAYIKKTEQKIGYYRHSAFYGKFLRLDNNWYLEISPHYRFTK
                     NGYWLSRFSDKQISTIKRVELNPAVLGQIVMWSEYLSRRPDMFTKEYPFLSFSDLESF
                     EVDFGFDDDSWLKREDEDNLMLDVVKSSVNDLPLFKGMK"
     gene            1305..2750
                     /locus_tag="PKI33_02200"
     CDS             1305..2750
                     /locus_tag="PKI33_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35839.1"
                     /translation="MKIEFIDEPELEFGTGRSVDIRFGLTNYHPVDFDNRIAPKSIRV
                     GIVGNNETIEGMAGWLEKCRNEIQAKQSNQPNLFPKFPGFRLDTGFNSELILDSSLQR
                     IIPQRQIDEALKAGNIKDKVINAVNLYLDELSYLNENAKVDVLICAIPEEVFNIGQPV
                     SDESDEDEAASYSRSISSIDFHDLLKAKAMRLGVPIQLVLPSTYDSKKRRTSRNNPAL
                     TRMLQDEATRAWNFHTAIYYKAKGIPWRLLRNPSDYSTCYVGVTFFKTDNDMLRTSVA
                     QVFNERGEGVIVRGGLARFDKEDRQVHLEQKDATTLLTNSLNLYKKEHGAFPARLVLH
                     KSSTYNKSEVDGFLESASSFSVDNVNLLSVEKSFTRLFRMGKYPPLRGTFLSLDKKQH
                     ILYTRGSVPFFATYPGMYVPKTFGFKTEIIEETPYFLAKEILALTKMNWNNTQFDGGE
                     PITTRASRQVGKILKYIEDGDPIAPSYRYYM"
     gene            3418..3999
                     /locus_tag="PKI33_02205"
     CDS             3418..3999
                     /locus_tag="PKI33_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35840.1"
                     /translation="MKYLSVALIALIMAGCGPSSPEMTATADMAMAETQTAAPTSTPT
                     LTPTSTATPTSTTTPILTLTPTLPHEAPGIDPGGMPESANSLFFESTPMFTFNLGGSV
                     KVNYIVFLEIKKELPKDAILEVHFQNPADPSTAYVVVVTDLTGEKIMVESKKFPMSNF
                     ECRNYWIDVHIYADRESIYELGTHVQWTSSSFC"
     gene            complement(4094..4303)
                     /locus_tag="PKI33_02210"
     CDS             complement(4094..4303)
                     /locus_tag="PKI33_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNM35841.1"
                     /translation="MIGEAASRLPKEFTARFPEIPWVDIIGFRNIAVHEYFSIRWDIA
                     WIAATEEVPVLREQVEKILREEFAE"
     gene            complement(4293..4439)
                     /locus_tag="PKI33_02215"
     CDS             complement(4293..4439)
                     /locus_tag="PKI33_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35842.1"
                     /translation="MRPEKLYLTDIVEAAQAIERFVMGESYYEFEQNEMMNSAVLQKI
                     DRDR"
     gene            complement(4429..4746)
                     /locus_tag="PKI33_02220"
     CDS             complement(4429..4746)
                     /locus_tag="PKI33_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNM35843.1"
                     /translation="MDEKTSSRVKIRVPKKELADFCRRYNVQKLALFGSVLGNNFRAD
                     SDVDVLVSFQPNAKIGFITFSRMQRELAELFNRPVDLVPMDGLKPVIRDSVLSDIEEV
                     YAS"
     gene            complement(4763..>5301)
                     /locus_tag="PKI33_02225"
     CDS             complement(4763..>5301)
                     /locus_tag="PKI33_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNM35844.1"
                     /translation="AAVERLFSYAAWADKYGGTVQETTLRGITVAVNEPVGVIGIACP
                     DEMPLLGFVSLMAPAIARGNTVVMIPSQKHPLSATDFYQVLDTSDVPGGVVNIVTGDR
                     DHLLKTLVQHEDVDSVWYFGSADGSYHVENESAANMKRTWVSYGSSRNWLDREQGEGH
                     EFLHEATQVKNIWVPTGE"
BASE COUNT         1624 a         1218 c         1085 g         1374 t
ORIGIN      
        1 tttttgaggt gctcgactct cattttactt ggtcacattt tccaaagcag gtcaaaatcg
       61 acgacgccga tattaagcag tggatgatat cgctgcccct tacaaatcta cctagaataa
      121 ctcagtcaat ctcgaatcta ccaacctctc ccgacgagaa agagctggaa gaagctataa
      181 gagagacgat tacgacctct tggaaaagtc tctcttattc gcaaatcgat ctagcagtta
      241 atacattcat gaattgtatt cgtgaagcgt tactgccgct gaaaaaacaa acattgctaa
      301 tagtagggcg tgctgttcta agaactgaag ataaaatcaa tttacttatc aagcttacag
      361 agaaattaat agaacagtct tcaaaaccat ccaccacaaa cacaaccaat cctgataaac
      421 aattctcaaa gccagacaca cgtcaggaaa cgctatattc aaacttattg aaaattgaga
      481 gctacgccaa aagaatatac gttgccccga cacacgcacg cgggcggaat gaaatattta
      541 gaaacctact taagttaagt gggcacgcca acagcgaatt tatacttaag aataaaacta
      601 ttatttcttt tcacgacttg tgttcatatc catggactga agtatgcgac tcatcattgg
      661 ctattgagta tagtagcgca gagtgggcac tttcagaaaa aatagataaa agaaatgaat
      721 ttatcgactt attaaacaaa tctattgaag caaaattgta cccagaagta ttgtttgatc
      781 gagaagaaaa atactattat tttgcaccga cagaagattt gcgagacaaa gagctatcgt
      841 acccaagctt acagaaaaac acttctcgtt ttgtgttcaa agcttacata aagaaaactg
      901 agcaaaaaat cggatattat cgtcactcag cattttacgg aaaattccta cgactagaca
      961 ataattggta tcttgaaatc tcgccacatt atcgtttcac taaaaatgga tactggctat
     1021 cgagattttc agacaaacag atttcaacca tcaaacgagt tgaattaaat cctgcagttt
     1081 taggtcaaat agttatgtgg tctgaatact taagccgacg cccagatatg tttacgaaag
     1141 agtatccatt tttatctttt agcgatctag agtccttcga agttgatttt ggtttcgacg
     1201 atgattcgtg gctaaaacgc gaagatgaag acaatctaat gttagatgtg gtcaaatcct
     1261 ctgtgaacga cttgcctctt tttaaaggga tgaaataatt tctcatgaaa atagaattta
     1321 tcgatgagcc agaacttgaa ttcggaacag gtcgcagtgt ggatattcga tttggtttaa
     1381 ctaattatca ccctgtagat tttgacaatc gaattgctcc caaatcaata agggttggca
     1441 ttgttggaaa taacgaaaca atagaaggaa tggctggctg gcttgaaaaa tgtcgtaacg
     1501 aaattcaagc taaacaaagc aatcaaccta atttattccc aaaatttcca gggtttaggc
     1561 ttgatactgg ctttaattcg gagttgatac ttgactcgag tctacaaaga attattcctc
     1621 aaagacaaat agacgaagca ttaaaagcag gcaatatcaa agataaagta attaatgcgg
     1681 taaaccttta cctcgatgaa ctttcttatc taaacgaaaa tgcaaaggtt gatgttctga
     1741 tttgcgcaat tcccgaagaa gtcttcaata ttggacaacc agtatcagat gaatccgacg
     1801 aggatgaagc ggcaagttac agcagaagca tttcttcaat tgactttcac gatctgctaa
     1861 aagcaaaagc catgagattg ggagttccaa ttcaattggt tttaccatct acctatgact
     1921 cgaaaaagag gcggacatcg cgtaacaacc ccgctcttac gcgcatgttg caagatgaag
     1981 cgactcgagc ttggaatttt cacacagcca tatactataa agcaaagggt attccatggc
     2041 gtttattgag gaatccatca gattattcca cgtgttatgt tggagtaacc tttttcaaga
     2101 cagataatga tatgctccga actagtgttg cacaagtttt taacgaaaga ggagaaggtg
     2161 taattgttcg aggtggttta gctcgttttg ataaagaaga taggcaagtc catttagagc
     2221 aaaaagacgc aaccacatta cttacaaact cattaaattt gtataaaaaa gagcatgggg
     2281 cattccccgc acgcctcgtt ttacataaaa gttcaactta caataaatct gaggtcgatg
     2341 gctttttaga aagtgcctct agtttcagcg ttgataatgt taacttattg agtgtggaaa
     2401 agtcattcac aagactattc cgaatgggga aatacccacc attacgggga acatttttat
     2461 ctcttgataa aaaacagcat attttatata caagaggtag tgttccattt ttcgcaacct
     2521 atcctggaat gtacgttcct aaaacttttg gttttaagac cgaaataata gaagagacac
     2581 cttattttct agcaaaagaa atcctagcac ttactaagat gaattggaat aatactcaat
     2641 ttgatggcgg agaaccaata accactcgtg catctcgaca agttggtaaa attttgaagt
     2701 atatagaaga tggagatcct atagccccat catatcgata ctatatgtaa aaacaataat
     2761 acctccgagc ggacaactcg gaggtattac tttccactca ctactttcta cgatcactcc
     2821 cccgacggca cccagatatt cttgacctgc gtcgccttgt gcaggaactc gtgaccttcg
     2881 ccttgctcgc ggtcgagcca attgcgggac gagccgtaac cgacccaaat ccgctgccta
     2941 gcaagcataa catccgattg cctatacgca aatcgaaaca gggggaacaa gaagtcctct
     3001 caggggagga ttggaaacga gtcagggaag gaattaaacc cttccttgag aggatcagaa
     3061 acctcccttg actcgtttta aaaaacatct ataagttttt taaaaacttc tagatgtttt
     3121 cccaaagact tctaaatgtt ttttaaaaac ttcaagaagt ttttccactc aaaacagccc
     3181 atttttcaca aaaaaaggcc tcgaaagcgc ctcgaaattc tgtcccatcc tgtccctggt
     3241 caaacccggt ctggaggcgt ccccacacgg caagatggat gagaacaggg gaaaattcta
     3301 ttacgcaggc caaaattaaa aattggcggt tattggcagt taaaagccgg ggaaaggttg
     3361 tataacgcca taatcaaaac aatcgcatca aacggaatag ccaacaggag aattaccatg
     3421 aaatatttat ccgtagcatt aatcgctttg atcatggctg ggtgcggccc gtcatcgcct
     3481 gagatgacag ccactgccga tatggcaatg gcggaaactc aaaccgcagc ccccacatcc
     3541 acgcctactt tgactccgac atcaaccgca accccaacgt caaccacgac cccgatattg
     3601 accttgactc ccacacttcc acatgaagcg cccggcattg acccgggtgg catgccagaa
     3661 tctgcaaact ctttgttctt tgaaagcacg cccatgttta cattcaacct tggcggttct
     3721 gtaaaggtta attatattgt ttttcttgag ataaaaaagg aattacccaa ggatgcaata
     3781 ctcgaggtcc atttccaaaa cccagctgac ccgtcaacgg cctatgttgt ggtcgtaaca
     3841 gacctaacgg gtgaaaagat aatggtcgaa tccaagaaat tccccatgtc caatttcgaa
     3901 tgcaggaact actggatcga tgtccacatc tatgcagaca gggaatcgat ctacgaatta
     3961 ggcacacatg tccaatggac aagttccagt ttttgctaaa aaacagacct ctgaggccag
     4021 cctcagaggt catctactct tcaatctcta cttccctgct cttccctact cccccgttgg
     4081 cacccagata ttcttattct gcaaactctt cccgcaaaat tttctcgact tgttccctca
     4141 acacagggac ttcctctgtc gccgcaatcc atgcaatatc ccaacgaatg gagaaatact
     4201 catggacggc gatattgcga aaaccgataa tatccaccca cgggatttca ggaaatcgag
     4261 cagtaaattc tttcggcaaa cgggatgcgg cttcaccgat cacggtcaat tttttggagc
     4321 acagcgctat tcatcatctc attttgctcg aactcataat aactctcgcc catgacaaag
     4381 cgctcaatgg cttgggcggc ttcaacgata tccgtcaggt aaagtttctc aggacgcata
     4441 cacttcctcg atatctgaga ggacagagtc gcgtatgacg ggcttcaaac cgtccatggg
     4501 aaccaagtcg acggggcgat tgaacaactc ggcaagttcg cgctgcattc gagagaaagt
     4561 gatgaacccg atttttgcat tgggttgaaa ggacaccagc acatccacat cactatccgc
     4621 gcggaagtta ttcccaagca cagaaccgaa taaagccaat ttctgcacgt tgtaacggcg
     4681 gcagaaatca gccaattctt tcttgggaac tctgatcttc acgcgggaag aagttttctc
     4741 gtccatgcct taattatacc tgtcactccc ccgtcggcac ccagatattc ttgacctgag
     4801 ttgcctcgtg caggaactcg tgaccttctc cttgctcgcg gtcgagccag ttgcgggacg
     4861 agccgtaact gacccaagtc cgcttcatgt tggcggcgga ttcgttctcg acgtggtaac
     4921 ttccgtctgc ggagccgaag taccaaactg agtcaacgtc ttcgtgttgg acgagggtct
     4981 tgagcaaatg gtcacggtca cctgtgacga tgttgaccac gccgccaggc acatcagacg
     5041 tgtcgagcac ctgatagaag tccgttgcgg agagcggatg tttctgactg gggatcatca
     5101 ccacggtgtt gccgcgtgca atggctggcg ccatgagcga gacgaaacca agcaggggca
     5161 tttcatcggg gcaggcgatg ccgatcacac ccacaggttc attcaccgcc accgtgattc
     5221 cgcgcaaggt ggtctcttgc accgtaccgc cgtatttatc tgcccatgcc gcataactga
     5281 agagacgttc caccgccgcg t
//
LOCUS       DAOREF010000069         5271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8726_length_5271_cov_5.064609, whole genome shotgun sequence.
ACCESSION   DAOREF010000069 DAOREF010000000
VERSION     DAOREF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8726_length_5271_cov_5.064609"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1593)
                     /locus_tag="PKI33_02230"
     CDS             complement(139..1593)
                     /locus_tag="PKI33_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35845.1"
                     /translation="MKRQTIYIALAALILPILLRGLWFYRGLPAQRPEIATPDYASFE
                     RPAVSVSTPDLENVKQLGGSVLIDAAHTNQFTMTEIDSFIEAIRVRGGTVKTITDATM
                     LETELKTSSAYITVSPSSAFAESEVQALKKFADRGGKILAFAEPTRYFLGFDYVSGNP
                     IPYGDVSAVNSLLKSWDISVNNDYLYNTQKNEGNFRNVLFEDFGKSELTFGLNEVALY
                     GSRSVETDSGLILLQGSETNLSSMDDAHHPHTGGAALSEDGSVAVFGDFTFMASPYNT
                     YTDNATLIQNMADFALAGQQQQSLDVFPYLFKQTTVKVYVSSEIEKTSTMVSALGSLQ
                     STMAALNYKLEFVDSVPTSGDAILVGTFDAPDEFDTYLKKSDVEIDADLLSTVAFGEL
                     SSSGNGLLLFNSNKSGNTLVLLAGSSDDVISLLSVMGYGGLSSCLTSEQVAVCSVGSG
                     DYYYDDTSSDVSTNGTTTDSSTEPTPEATSTPAG"
     gene            complement(1606..2451)
                     /locus_tag="PKI33_02235"
     CDS             complement(1606..2451)
                     /locus_tag="PKI33_02235"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNM35846.1"
                     /translation="MSDADVRWERIRNILLLAVLVIIGLLIGSAIFPQPVIGVIRLED
                     AIYTYSAQNTIKQIQYAIDHSEVKAVVFVIDSPGGTVVDTESIYMELTRLREKKPVVT
                     VVNGMSASGGYYLSANTDYIFAKPTSLVGNIGVIGYLPPAPFVIEDVITTGPYKLWGA
                     PRDSDMRQVEMIKQGFFDAVRFGRGDKLTAEENVTFSGQIWAGIDALKLGIIDEFGTE
                     NDGSKKAAELAKVANYEIVDLAAPAGIDMSGGFFFMASADGVTLPYPKEAGMYLLYIP
                     PLPVK"
     gene            2600..3076
                     /locus_tag="PKI33_02240"
     CDS             2600..3076
                     /locus_tag="PKI33_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35847.1"
                     /translation="MPTPIKPAVKPGSAIAKPGTVSYVKFSDKLTASVTDIAKIIEQH
                     KEMIDSIQEVAIELTSSIGSLHALTVKYARTANQILDVLLPILKNLPIIPKNVMQLLV
                     NLESLTQKIIDNEKVTAKTITDVNSGLKTGDANKLKAHAGQLKEVTKTITAIIPKA"
     gene            3325..3633
                     /gene="rplU"
                     /locus_tag="PKI33_02245"
     CDS             3325..3633
                     /gene="rplU"
                     /locus_tag="PKI33_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNM35848.1"
                     /translation="MKFAIVESGGKQYRAVEGTTIDVDRLALELGTKFDFERVLLVAD
                     GDNVSVGTPTLSVKVSATVVDHVKGPKTLAFKYRPKKRIRVRGGNRAHYTRLKIESIG
                     "
     gene            3654..3914
                     /gene="rpmA"
                     /locus_tag="PKI33_02250"
     CDS             3654..3914
                     /gene="rpmA"
                     /locus_tag="PKI33_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNM35849.1"
                     /translation="MAHKVGGGSSRNGRDSNSQRLGVKRYAGQFVLSGNVLVRQRGTR
                     IKPGLNVMVGKDDTLFATADGVVMFDVYRGKKRVNIVPAEVE"
     gene            3914..4117
                     /gene="rpmE"
                     /locus_tag="PKI33_02255"
                     /pseudo
     CDS             3914..4117
                     /gene="rpmE"
                     /locus_tag="PKI33_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            4127..4423
                     /locus_tag="PKI33_02260"
     CDS             4127..4423
                     /locus_tag="PKI33_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNM35850.1"
                     /translation="MEEQKTREESTNVNNRSVDDLGLTPRATEGLKRAGILNIGQLVE
                     KFAEGDAALLAINGFGQSALTAAKKKLRAMDITLPEPPKVEKAEKAEKPAEAAE"
     gene            4525..5094
                     /locus_tag="PKI33_02265"
     CDS             4525..5094
                     /locus_tag="PKI33_02265"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNM35851.1"
                     /translation="MRHTHGLIEVVCGSMFSGKTDELIRRLVRATIAKQKVQVFKPAI
                     DIRYAVEKVASHTGSTFDAIPVEKASEILSKVAADTTVVGLDEAQFFDAEIVDVAQTL
                     SDRGIRVIVAGLDMDFRGEPFGSMPVFMAKAESVLKLHAICMVCGDEASRTQRLVNGK
                     PARYDEPVVIVGASELYEARCRLHHEVPR"
BASE COUNT         1397 a         1341 c         1217 g         1316 t
ORIGIN      
        1 ctgcaatgga tttggtttgg gcatccaagc cccaccattc ttgaatgacg atcaatgctg
       61 ggaagggacc ttcaccttct ggttttgcaa aatgtccttt tacttcacct agttgaatta
      121 attcgctcat agatacctct accctgctgg agttgatgtt gcttctggag tgggttcagt
      181 ggaactatct gttgtcgttc cattggtaga tacatcagaa ctcgtatcgt cataataata
      241 atcaccgctg ccaacactgc atacggctac ttgttcactg gtaaggcacg aacttaagcc
      301 gccatagccc ataacactta ataaagaaat gacatcgtct gacgagccag ccaaaagaac
      361 aagcgtattt ccgctcttgt tggagttgaa cagcagcaaa ccatttccag aactgcttaa
      421 ttctccaaat gcaacggtgc tgagaaggtc ggcgtcaatt tcaacatccg acttcttcaa
      481 gtaggtatcg aactcatcgg gcgcatcgaa tgttccaacc aaaatggcat caccagaggt
      541 cgggaccgaa tctacaaatt caagtttgta gttcaaagcc gccattgttg attgcaaact
      601 tcccaaagct gaaaccatgg tagaagtctt ttcgatctca gatgagacat acaccttcac
      661 ggtcgtttgc ttgaagagat aggggaagac atccaaactt tgttgttgct gacctgcgag
      721 cgcaaaatct gccatgtttt ggatgagagt cgcgttatcg gtataggtat tatatggaga
      781 agccataaag gtaaagtcac caaagacagc tacacttcca tcttcgctca atgcagctcc
      841 gcccgtgtgt ggatgatgcg catcatccat cgacgagaga ttggtctctg acccttgaag
      901 cagaatcaac cctgaatccg tttcaaccga gcgcgaacca tataacgcca cttcgttcaa
      961 gccgaaggtt agttcacttt tcccaaaatc ttcaaacagc acattgcgga agttgccttc
     1021 attcttttgg gtgttataga gataatcatt attaacagaa atatcccaac tctttagcag
     1081 ggaatttacg gcacttacat ctccatatgg gatcgggttt ccagagacat agtcgaaacc
     1141 aagaaaatag cgcgtcggct ctgcgaaagc cagaatcttc ccgccgcggt ctgcaaactt
     1201 tttgagtgcc tgcacttcgc tttcagcaaa tgccgaagat ggtgagacgg ttatgtacgc
     1261 gcttgacgtc tttagctcag tttccagcat ggtcgcatcg gtgatcgttt tgaccgtccc
     1321 tccacgcacg cggatcgctt ctatgaacga gtcaatttcg gtcatcgtaa attgattcgt
     1381 atgcgcagca tcgatcaaaa cactgccacc aagttgcttc acattttcca agtcaggcgt
     1441 gctcacagag acagccggtc tttcaaaaga tgcataatct ggcgtagcaa tttcaggtcg
     1501 ctgtgcagga agcccacggt aaaaccatag tccacgcaag agaatgggga ggattaaagc
     1561 agcgagcgca atataaatag tttggcgttt catttcatcc tcctattatt taaccggaag
     1621 aggcggaata taaagaagat acatgcccgc ttcctttgga tacggcaatg tcacgccgtc
     1681 ggcacttgcc ataaagaaaa agccgccaga catatcaatg ccagccggag cagccaaatc
     1741 tacaatttca tagttggcga cttttgccaa ttctgcggct ttcttggagc catcattttc
     1801 cgtcccaaat tcgtcaatga tgcccaactt gagcgcatcg atccccgccc agatctgtcc
     1861 gctaaacgtg acattttctt ccgctgtcag cttatctccc cgcccaaagc ggaccgcatc
     1921 aaagaagcct tgcttaatca tctccacctg acgcatatcc gaatcacgcg gcgcacccca
     1981 taacttatac ggtcccgtcg taatgacatc ttcaatcaca aatggggcag ggggcaagta
     2041 gccgatcaca ccaatattcc caaccaatga tgtaggcttt gcaaagatat agtctgtatt
     2101 ggcagaaaga taatacccgc cgctggcaga catcccattg accactgtca ccaccggctt
     2161 cttttcgcgc aaacgggtca gctccatata aatggactct gtatccacca ccgttccacc
     2221 agggctatcg atcacaaata caaccgcctt tacttctgaa tggtcaatcg cgtattggat
     2281 ctgtttgata gtgttttgtg ctgaataggt ataaatcgca tcttccagcc gtatcacccc
     2341 aatgactggt tgagggaaga ttgccgagcc tattagcaac cctatgatta caaggactgc
     2401 caatagcagt atgttccgaa ttcgttccca acgtacatcc gcatcgctca tgggtttctc
     2461 ctctataagt tggaattcgc tttcaggata tacgagaaaa catgctttgt caacaaggtt
     2521 gttttgcaaa acgatataca gtatatttgg catatcttgc tgcatgtgca gcacaaattt
     2581 tttcaggagg tattttctca tgccaacccc aatcaaacct gcagtaaaac ctggaagcgc
     2641 cattgcaaaa ccgggcaccg tatcctacgt caagttttca gataaattaa ccgcctcggt
     2701 cacagacatt gcaaagatca ttgaacaaca caaagaaatg atcgactcga ttcaagaagt
     2761 tgccatcgaa ttgacctctt ccatcggctc cctgcatgct ctcaccgtca aatatgcccg
     2821 caccgcgaat cagattctcg atgtgttgct ccctattctc aaaaacctgc ccatcatccc
     2881 caagaatgtg atgcagttgt tggtcaacct tgaaagcctt acccaaaaga tcatcgacaa
     2941 tgaaaaggtc accgccaaga ccattaccga tgtcaattct ggcttgaaaa caggcgacgc
     3001 caataaattg aaagcgcatg cgggtcaatt gaaggaagtg accaagacca tcacggctat
     3061 tatccccaaa gcctagcccg cctctttctg gacctaaacc tccggcaccc aaccaggcag
     3121 gagccccgcc ttggcagttc tccctcgccc aaatctcgtt gacaagtccc agactctgca
     3181 tgtataatac cgtccgcttc caccccggag gtcagattcc ggggtagttg tttgtttaca
     3241 accccagctt ccccagaagt aggcgaaggg tgcaagatcc ccgcgaactt tctcggcgaa
     3301 gtgaatgact ggagaaaata gaaaatgaaa tttgcaatcg ttgaaagcgg cggcaagcaa
     3361 tatcgtgccg tcgaaggcac cacaatcgat gtggatcgcc tcgcccttga attgggcaca
     3421 aagtttgact tcgagcgtgt tttgctcgtg gctgatggag acaacgtctc cgtgggtaca
     3481 ccaaccctca gtgtgaaagt ttctgccact gtggtggacc atgttaaggg acccaagacc
     3541 ctcgcattca agtatcgccc gaagaaacgc atccgtgtgc gtggcggcaa tcgcgcccat
     3601 tacacccgtt tgaagatcga atccatcggt taagaaataa aggtaggtag acaatggctc
     3661 ataaggtagg cggtggttca agccgcaacg gtagagatag caattcccag cgcttgggtg
     3721 ttaagcgcta cgccggtcag tttgtgcttt ccggcaacgt gttggtgcgc cagcgcggaa
     3781 cccgcatcaa gccgggtttg aacgtgatgg tcggtaaaga tgatactttg ttcgccactg
     3841 ccgacggtgt ggtgatgttc gatgtctatc gcggcaagaa gcgcgtgaac atcgttccgg
     3901 ctgaggtgga ataatgagag cggatattca tcctacagtg tacgatgcca aggtcacttg
     3961 tgcttcctgc ggcaacactt ggattactac ttccaccaag aaagaattac gcgtggacgt
     4021 gtgctccaac tgccatcctt tctacaccgg tgaatctgcc aagatcctcg atgtcgaagg
     4081 tcaggtagac cgcttctata agaagctttc tgctcgccag tcctatgtgg aagaacagaa
     4141 gacccgcgaa gaatccacca atgtcaacaa ccgctctgtc gatgatctcg gtctcacccc
     4201 gcgtgctacc gaaggtctca agcgcgctgg cattttgaac attggtcagc ttgttgaaaa
     4261 attcgctgaa ggcgatgctg ctcttctcgc gatcaacggt tttggtcagt ctgctttgac
     4321 ggctgccaag aaaaagctcc gcgcaatgga cattactctc cctgaacctc ccaaggtaga
     4381 gaaggctgaa aaagccgaaa agcccgccga agctgctgag tagtcagtcc gcttcttatc
     4441 aggtaaacta aacaggcaga tgcaaaagcg tctgcctgtt tttatgaaaa aaacattttc
     4501 cccgaacggg gcaggagaat ttcaatgagg catacccacg gcttgatcga agttgtttgc
     4561 ggttcaatgt ttagcggcaa gacggatgaa ttgattcgac gactagtacg cgccaccatt
     4621 gccaagcaaa aggtgcaggt ctttaaaccc gccatcgaca ttcgttatgc ggtggaaaaa
     4681 gtcgcttcgc ataccggctc aaccttcgat gccattcccg tggaaaaagc cagtgaaatt
     4741 ctctcaaaag tcgcagcaga cacaaccgtt gtgggtttgg atgaggcgca gttctttgat
     4801 gccgagatcg tggacgtggc gcagaccctt tcagaccgtg gcattcgtgt catcgttgct
     4861 ggtttggata tggattttcg tggcgaacca ttcggctcca tgccggtgtt tatggcaaag
     4921 gcagaatctg tgttgaagct gcatgccatt tgtatggttt gtggagatga agcgtctagg
     4981 actcagcgtc tggtgaatgg caagcctgcc cgttatgatg aaccggtggt tattgtcggt
     5041 gcatctgagc tttacgaggc gcgctgccgt ctgcaccatg aagtcccacg ctaatgaaag
     5101 tccacattca tctttagtag tgataaacta acttaaactg gaacaaccaa ggaattactt
     5161 taatgcaaaa ctaccaggat ttcctcgccg aagtgctaat tgatacagcc gctttacaga
     5221 agcgtgttgc tgaattgggc aagcagatca gctctgatta tgaaggacag g
//
LOCUS       DAOREF010000070         5242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8791_length_5242_cov_5.159823, whole genome shotgun sequence.
ACCESSION   DAOREF010000070 DAOREF010000000
VERSION     DAOREF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8791_length_5242_cov_5.159823"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            872..1354
                     /locus_tag="PKI33_02270"
     CDS             872..1354
                     /locus_tag="PKI33_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35852.1"
                     /translation="MSKDYYIVGTKYGRGIEDVYPDMLALGAVSVGFSRQIDLSDWYG
                     ASEKDIIEHLKKLGESEISYHNLKRFLNIKAGDLIAIKRSGSPLGNMARLVIAGYAIA
                     EARDGKVYCHDPNGLGHLIYVKFLNTESDKEFSLGYGQAVHKLTRESHIRQIFSDYGA
                     "
     gene            1599..2594
                     /locus_tag="PKI33_02275"
     CDS             1599..2594
                     /locus_tag="PKI33_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HNM35853.1"
                     /translation="MPNKNELLHKAGKHKKDEFYTQLSDIERELKHYKNHLKGKVVYC
                     NCDDPRVSNFFHYFSYNFEKLGLKKLIATCYKNQNMDLFSQNNSEQAIYLEYVGDKNK
                     NSIPDPKEIGIKPLKGDGDFRSKECIELLKQADIVVTNPPFSLFREYVAQLIEYDKKF
                     IIIGHQNAISYREIFKLIMDNKLWLGYGFNRNMAHFINPNYEDYAADADHKEGMIRVS
                     GVVWFTNLDIQKRHEDLILYKTYRGNESEYPKYDYYDAINVDKTKDIPMDYKGQIGVP
                     ITFIDKYNPDQFEIIDGIGRYSMLTGPTKKTKGTYLTKVDGKPKYARIVIKNKRL"
     gene            2607..3689
                     /locus_tag="PKI33_02280"
     CDS             2607..3689
                     /locus_tag="PKI33_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNM35854.1"
                     /translation="MKIELKEITVRELTNGYQDNEENGVIGYGGKLDIRPPYQREFIY
                     KDKQRDAVIDTIIKKFPLNVMYWAVRADGNFEVIDGQQRTISVSQFVEGDFAFQNRYF
                     HNLQKDEQKQILDYKLMVYLCSGEDSEKLEWFKTINIAGEKLTDQELRNAVYAGSWVS
                     DAKRYFSKNNCPAYNMGNDYLSGAPIRQDYLETTIEWISNSSIEKYMAEHQHSPNANE
                     LWLYFQSVINWVKAVFPKYRNEMKGIQWGFLYNEFKDKNPDSKKLEKEITKLMLDEDV
                     TNKKGIYSYVLTRKEKFLNIRAFSDNQKREAYERQKGICVVCKQHFELSEMEADHRKP
                     WHEGGKTSAENCQMLCKEDNRRKSGK"
     gene            4016..4393
                     /locus_tag="PKI33_02285"
     CDS             4016..4393
                     /locus_tag="PKI33_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35855.1"
                     /translation="MITKLFSDGVYGDSDKALQAAQAYHRQAQIKFPPIEKPPFRETP
                     LRNNTSGYNGICETFSRTRKGEKISCWNVSWYNPPNKLNSKKFYFHDPQERKQALKEA
                     LQFRKEREAEILKQSRRRRKITA"
     gene            4436..4618
                     /locus_tag="PKI33_02290"
     CDS             4436..4618
                     /locus_tag="PKI33_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35856.1"
                     /translation="MAFVVFSGKVLVSRQATQQSVHLTLGILRKSQAVSHALAFFWLD
                     GFAVPTPAQVTQTVGR"
     gene            4646..5176
                     /locus_tag="PKI33_02295"
     CDS             4646..5176
                     /locus_tag="PKI33_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35857.1"
                     /translation="MKRYQFWCPKCRKGFYEHEVGRREVDTMFGEGRWWFCQACDTLV
                     ISYANQDLDVHSILFALGDQAMNSVWQVSGVDCFGENAEELWLIDDNKQSVRGNDLLR
                     ITTGITQTIDGDFHAFDNCSTSHWFYMRAWDGSGFYVETNDHKVKERLDARFRNVEDV
                     AETQPPYEGLFIPRGD"
BASE COUNT         1714 a          992 c         1131 g         1405 t
ORIGIN      
        1 agcgtgcacc tgacgctggg gattctgcgg caatctcaag cagttttcta cgcctgagca
       61 tttttctggc tggacggctt cgccgtcccc gccccagcgc aggtaacgca tgccgttggg
      121 cagcttaact gcaacataga aaaacattct tgaatcatat gtaccagcaa cgtgctctaa
      181 taaatggttt cacgctaaaa agattcgtaa ctcaaacagc aataatcctt ttgttgggtg
      241 gaattatttc cgcctcaata gttatcttag actgggatat ggaattgttg ttcatcgcct
      301 gccaaattgt gggatataca ttgttcacta cagtttcagt catccattct cgccttacac
      361 agttttgggt gatgatagtc gcagatggtc ttgttggatt aataccgttt attattttgg
      421 tgggttattg ttactttcat attgagaata ccgcttgcag tgtgcgttca gtaaaaggta
      481 atagtattgc gttctgggtt ttcccatcca taataatgtg gattctttgg attcttgctc
      541 ctcttggaaa aacgagaaga aggtatggtt aagtgcgctg gaaattgtat tgggttgcac
      601 tatactactt tgaaccaatt tcaatataat ggagtttgaa ggcaagaggc tgcccaacac
      661 cgcgtgcagc cgacttgggg tacgcaccgc gttatcttgg catttttctg gcttggagtt
      721 tttcccgttt cgacggcgaa tccacgctcc aaccccaagc ggctaacgcc agccgttggg
      781 ctgttccttg caaaacaaaa taattactga aaatcaaaaa catcgccatt aatcaagatt
      841 gagaatcggg ttgcaaagga gtgcaatcac aatgtcaaaa gattactata ttgttggcac
      901 aaagtatggg cgcggcatag aagatgttta cccagatatg cttgctcttg gcgcagtgag
      961 cgtcgggttt tctcgccaaa ttgatttaag tgattggtat ggcgcaagcg aaaaagatat
     1021 tattgaacat ctaaaaaaat taggggagtc ggaaatttca taccataact taaaacggtt
     1081 tctaaatatc aaagcaggcg accttattgc aattaagcgt agtggctcgc cattaggcaa
     1141 tatggcgcga ctggtaattg caggttatgc tattgcagaa gcaagagacg gcaaagtata
     1201 ttgccatgac cccaatggtt taggacattt gatctatgtt aaattcctaa atacagaaag
     1261 cgataaagaa ttcagtttgg gatatggtca agccgttcat aagttaactc gtgaatctca
     1321 tattcggcaa atattctctg attatggcgc gtgaaatgat atgtcgccaa acaatacaag
     1381 agtctctaaa cataaaaaca gcccaacaaa gcgtgcaccc gacgctgggg attctggcgc
     1441 gattccaagc atttttctac gcctcagcat tttcccagtt ggacggcgtt ccgccgcccg
     1501 ccccagcgcg ggtaacgcaa accgttaggt ggctgtcttg cataacaaag taaaatagtg
     1561 gttggaaatt aaaaacattg caaaccgaga tttaggatat gccaaataaa aacgaacttt
     1621 tgcataaagc aggtaaacat aaaaaagatg agttttacac tcaattatct gatattgagc
     1681 gagaactaaa gcattacaaa aaccatctta agggtaaggt ggtttattgc aattgcgatg
     1741 acccgcgagt tagtaatttc ttccactatt tctcgtataa ttttgaaaaa ttgggtttga
     1801 aaaaattaat tgcaacgtgt tataaaaatc aaaatatgga cttgttcagc caaaacaact
     1861 ctgagcaagc catttattta gaatacgttg gagataaaaa caaaaatagc atacctgacc
     1921 ccaaagaaat cgggataaaa ccgttaaaag gtgatggaga ttttcgtagt aaagaatgta
     1981 tcgaactatt aaaacaggct gacattgtag taactaatcc gcctttttct ttattcagag
     2041 agtatgtcgc ccaactaatt gagtacgata aaaaattcat cattatcgga catcaaaacg
     2101 caatttctta cagagaaata ttcaaactca ttatggataa taaattgtgg ctcggatacg
     2161 gctttaatcg taacatggca cattttataa atcccaatta cgaagattat gcagcggacg
     2221 cagaccataa agaaggcatg attagagttt ctggcgtggt atggtttact aaccttgata
     2281 ttcaaaaaag gcatgaagat ttaattctct acaagacata tcgtggaaat gaaagcgaat
     2341 acccaaaata tgattattat gacgctatca atgtagataa aacaaaagat atacctatgg
     2401 attacaaagg tcaaattggt gttccaataa cctttattga caaatacaat cctgaccagt
     2461 ttgaaatcat tgacggaatc ggcagataca gcatgttaac tggaccgact aaaaaaacaa
     2521 aaggaacata tcttactaaa gttgatggca aaccaaaata tgctcgtatc gttataaaaa
     2581 ataaaaggct ctaaattgag taaacaatga aaatcgaact caaagaaatt accgtccgtg
     2641 aattgaccaa tggataccaa gacaacgaag aaaacggcgt tattggttat ggtggcaaat
     2701 tagatatacg cccgccttat caaagagagt ttatctacaa agataaacaa agagacgcag
     2761 taattgacac gattataaaa aaatttccgt tgaatgtaat gtattgggct gttcgtgcag
     2821 acggcaactt tgaagttatt gacggacaac aacggactat ttcagtcagt caatttgtag
     2881 aaggtgattt tgcgtttcag aatagatatt ttcacaattt gcaaaaagat gagcaaaaac
     2941 aaattttaga ttacaaactt atggtttatt tgtgcagtgg cgaagatagc gaaaaacttg
     3001 aatggttcaa aactattaat attgcaggag aaaaacttac cgaccaagaa ttgagaaatg
     3061 ctgtctatgc tggttcatgg gtttctgacg caaaaaggta tttcagcaaa aataattgtc
     3121 cagcctataa catgggcaac gattatttaa gcggtgcgcc aatcagacaa gattatttag
     3181 aaaccacaat cgaatggatt agcaacagtt ccatagaaaa atatatggct gaacatcagc
     3241 atagcccaaa cgctaatgaa ttatggcttt attttcaaag cgttatcaat tgggtaaaag
     3301 cggtgtttcc aaaataccgt aatgagatga aaggtatcca atggggattc ctttacaacg
     3361 agttcaaaga taagaatcca gattctaaaa aacttgaaaa agaaattaca aagttaatgc
     3421 tagacgaaga tgtaacaaat aaaaaaggta tttattcata cgtcctcact cgaaaagaaa
     3481 agtttttgaa tatccgtgca tttagcgata atcaaaaacg tgaagcatac gaaaggcaaa
     3541 agggaatttg cgtagtttgc aaacagcatt ttgagttaag cgaaatggaa gcagaccata
     3601 gaaagccctg gcacgaaggc ggtaaaacat ccgctgaaaa ttgccaaatg ttgtgcaagg
     3661 aagataaccg aaggaaatct ggaaaataaa gttgtgttaa agtcaaacgc cacctaacaa
     3721 agcgtgcact tgacgggtgg gattctgcgc cattttcggg cttttttcta cacccgaaca
     3781 gaatcctgct ctcgaagttt tctctcatcc cgcccacccg caagtaacgc caaccgttgg
     3841 gttgcccccg tgcaaaacat aaagagcctt gaataattgt cagatataat tcctttcatc
     3901 tctctattaa tgaaaggaat ttgacagaca aatgaatact tactctttga ggggtatcag
     3961 caggatacac tcaaaggggg ctggctggtt agtcaggttg tatcgaaatg ctaaagtgat
     4021 taccaaactc tttagcgatg gcgtttatgg tgactccgat aaagcattac aagcagcaca
     4081 ggcttatcat cggcaggcgc aaataaaatt cccgcccata gaaaaaccgc catttcggga
     4141 aacgccactt agaaataaca catctggcta caatggcatt tgtgagacgt ttagccgtac
     4201 tagaaaaggc gagaaaatat cgtgctggaa tgtcagttgg tataacccgc caaacaagtt
     4261 gaatagtaaa aaattctatt ttcatgaccc gcaagaacgt aagcaagccc tgaaagaagc
     4321 acttcaattt cgtaaagaac gagaagccga gattttgaag caatctcgca gaaggcgaaa
     4381 aatcacggca taagttattt caagaatcac gcctgaaaaa attgcattgg gtcttttggc
     4441 gtttgtagtt ttttcaggca aggttctggt aagcaggcag gcaacccaac aaagcgtgca
     4501 cctgacgctg gggattctgc gcaaatctca agcagtttca cacgccttag catttttctg
     4561 gctggacggc ttcgccgtcc ccaccccagc gcaggtaacg caaaccgttg ggcgctaaac
     4621 agatcatagc gagaattgtt ttcttatgaa aagatatcag ttttggtgtc cgaaatgcag
     4681 aaaaggattc tacgaacatg aagttgggag gagagaggtt gataccatgt ttggggaagg
     4741 acgttggtgg ttttgccaag cgtgcgatac tttggtcatt tcatatgcca accaagactt
     4801 ggatgtacac agcatactct ttgcactggg tgatcaagca atgaattctg tatggcaggt
     4861 aagtggtgta gattgtttcg gagaaaacgc agaagaatta tggttgatag atgataacaa
     4921 gcagtctgtc cgaggaaatg atttgcttcg tatcacaaca ggtatcaccc aaaccattga
     4981 tggtgatttt cacgcatttg acaactgctc aacatctcac tggttttata tgcgtgcttg
     5041 ggatggtagt ggattttatg ttgagacaaa tgaccataaa gtcaaggaac gtttggatgc
     5101 ccgatttcga aacgttgagg atgttgcaga aacacagcct ccctatgagg ggctatttat
     5161 tccacgtggc gattaaaagc caaaaagcgc ccaacaaagc gtgcacctga cgctggggag
     5221 tctgcgcaca tcccaagcag tt
//
LOCUS       DAOREF010000071         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8820_length_5234_cov_5.000772, whole genome shotgun sequence.
ACCESSION   DAOREF010000071 DAOREF010000000
VERSION     DAOREF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8820_length_5234_cov_5.000772"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKI33_02300"
     CDS             <1..1154
                     /locus_tag="PKI33_02300"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35858.1"
                     /translation="KEISLWDVQYTLMREEVDMNDAGDRALYDLRVERNTFAAKAALQ
                     YMQANKPDVVLIPNGLILEMGIVFRVARYLGIDAVTYEFNDQREQIWLAQNSSIMQQD
                     TDYLVEARCSQPMTDAMFERVADLENARRGARVWGKSKRLWQYVSSQGADATRKQVGL
                     DDRPVVMLAANVLGDSLTLGRDIFASSMTEWITKTVQYFAKRTDVQLVIRVHPGEKLV
                     PQAKSMATVVKEALPELPSHIHVIGALDKVNTYDLIEIADVGLAYTTTVGLETAMNGR
                     PVISCGQTHYRGRGITIDPNTWDEYYATLEKVLSNIPAHQMNEKQIEFAWNYAYRFFF
                     EYPRPFPWRLMNFWDDLAEHPLESVLSPEGMARFKKTFDFLVCEPFTWD"
     gene            1164..1988
                     /locus_tag="PKI33_02305"
     CDS             1164..1988
                     /locus_tag="PKI33_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNM35859.1"
                     /translation="MTNETKQKVREFYDEIGWMQEEDGNYQNARYEDLRPVSREYIHK
                     TRLRVMNGLIPTGRFILDAGSGPVQYEEYKTYSAGYEKRVCLDISIQALREARTRIGD
                     HGLFVVGDLANLPFKKDAFDGAVSMHAIHHLALPEHPKAYAEIHRVLAQGRTAAIVNG
                     WHNPLLIRMAEPLIGLMRKLSGRSEKKKKEWLSEEDPAGTFVQKMTPEWLKKEIGSKM
                     TIEIKPWRSMSTRIMRWFVRPFGGRAFLRFVFWLEGVFPKFFAENGQYPLIVVRKS"
     gene            2031..4052
                     /locus_tag="PKI33_02310"
     CDS             2031..4052
                     /locus_tag="PKI33_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35860.1"
                     /translation="MKKKNFPEILSLLLISAIVYLPHIGSLTYYKDDWYYIYDGMLGG
                     AKIFHEMFRIDRPARGFFFEWYYTLFGPNPLPWHLSVFVWRALAALGALWIFNLLWKE
                     NRKFNFIAALLFAIYPGYFWWISAIEYQPMIASLALQVFSIAFTLKAIQSAKIAEKAA
                     YGIGSILTGWSYIALVDYAIGMEAFRFIAVYLLIAHDINDTFWKRLLAALKAWAWTVL
                     IPFCIVVWRIFFFTNERKATDIGGQLSVFFSNPVSVGIDWLLQIYNSVLNLSVQAWIN
                     QFPTFIQSMRLRDITLSIVLAVIVTLLVVFAEKQIKQSAETKDEGGKQAKVTNEALIL
                     GGLGMIFGVLPVVMANRYVNISGFSHYGLPASLAAAVFMAGIIEIIALSSVRKAVLYG
                     VIVFASLSHFGIATNAAMEEKALQEFWWQVSWRVPDIQPGTTLVIYYPLPGMGDGGAA
                     LMEAANVIYFPEFTGDAIVHYPLSGLTLNSENLPGILDGTQIVESGYRSHTYTFDYTN
                     ILVMSQPTLNSCVHVMNASAPLISEYDPVDVSLVASQSKIENVIADAEPVSPPEYIYG
                     KEPDTGWCSYFQAADLAAQTGDWERVAALGDEAIALSFAPEDRVEWLPFLKAYAIRGD
                     AETLKQLSKRVIGEKIIRLQACEMLSSIEQPLNDDVREMIDINYCKGSE"
     gene            4052..4846
                     /locus_tag="PKI33_02315"
     CDS             4052..4846
                     /locus_tag="PKI33_02315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM35861.1"
                     /translation="MSKLTDKEYLTQKQYKDSTNLDVRVAIHKRFSTNSYGWFNWVFD
                     SFSALPENATILEIGCGTGELWKECESRIPKNWALTLTDISDGMLDAAWRNLIRIHRG
                     IKFEKVDSQSIPYADKTFDAVIANYMLYHVADRKKAFAEIKRVLKDDGVLFSATAGAN
                     HLREMYEWVFRVSGGRHGQIALQFTLENGKEQLQEFFPRVELSRYPDSLQITDVDMLI
                     AYVRSMASVELSEEEIQKLEQEWSEILVKDGAIHISKDAGLFQALQ"
     gene            4933..>5234
                     /locus_tag="PKI33_02320"
     CDS             4933..>5234
                     /locus_tag="PKI33_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNM35862.1"
                     /translation="MDWKNQVVLVTGGTGSFGKKFTRILLEEKQPKKIIIFSRDELKQ
                     HEMQVGGYNDPRLRYFIGDVRDRERMVRAMHGVDIVVHAAALKQVPACEYNPMEAI"
BASE COUNT         1317 a         1109 c         1341 g         1467 t
ORIGIN      
        1 tcaaagagat ttccctttgg gatgtgcaat acactttgat gcgcgaagaa gtggatatga
       61 acgacgcagg ggatcgcgct ttgtatgacc tgcgtgtgga acgcaatacg tttgctgcca
      121 aagccgcgct tcaatatatg caagcgaata aaccggatgt ggttttgatc ccgaacggtt
      181 tgatcttgga aatgggaatt gtgttccgtg tggcgcgtta tcttggcatt gacgccgtca
      241 catatgaatt caacgaccaa cgcgaacaga tttggttggc gcagaattca tccatcatgc
      301 agcaggatac cgattatttg gtagaggcac gctgctctca gccaatgacg gacgctatgt
      361 tcgagcgtgt agcagatctt gaaaatgctc gtcgtggtgc acgtgtgtgg ggcaagtcca
      421 aacgcttgtg gcaatatgtc tcttcgcaag gggcagatgc cactcgcaaa caagtaggtt
      481 tggatgaccg tccagtagtg atgctcgctg cgaacgtgct gggtgatagc ctcacgcttg
      541 ggcgtgacat cttcgcctct tccatgaccg aatggattac caagaccgtt caatattttg
      601 ccaagcggac ggatgttcag ttggtgatcc gtgttcatcc gggtgaaaaa ttggtgccgc
      661 aagccaagtc tatggcgacg gtagtgaaag aagctttgcc cgagctgccg agccatattc
      721 acgtgattgg cgcgttagat aaagtcaata cgtatgattt aatcgaaatt gctgatgtcg
      781 gtttagccta caccacaacg gtcggactcg aaaccgccat gaacggacgc cccgtcattt
      841 cttgcggaca aacccattat cgtgggcgtg gcattacgat tgacccaaat acgtgggacg
      901 aatactatgc cactcttgaa aaagtgttaa gcaatattcc tgcccatcaa atgaatgaaa
      961 agcaaattga atttgcgtgg aattatgcct atcgtttctt cttcgaatat ccacgcccct
     1021 tcccatggcg cttgatgaac ttttgggatg atcttgccga gcaccctctg gaaagcgtgt
     1081 taagtcctga aggcatggca agatttaaga agacctttga ttttctggtt tgcgaacctt
     1141 ttacatggga ttaagaaaaa taaatgacta acgaaaccaa acaaaaagtc cgtgaatttt
     1201 atgatgagat cggctggatg caggaagaag acggcaatta tcagaatgcc cgttacgaag
     1261 acttgcgccc cgtctcacgc gaatacatcc acaagacccg tctgcgcgtg atgaatggac
     1321 tcatccccac cggtcgcttc attcttgacg ccgggtctgg tcctgtccag tatgaggaat
     1381 acaagaccta ttctgcgggc tacgaaaagc gcgtctgctt ggatatttcc attcaagcct
     1441 tgcgcgaagc acggactcgt attggggacc acggtctatt tgtcgttggg gatttggcga
     1501 atctgccttt caaaaaggat gcttttgatg gcgcggtttc tatgcacgcc attcatcatc
     1561 ttgccttgcc cgaacacccc aaagcttatg cggaaattca ccgcgtcctt gcacagggtc
     1621 gcacggctgc catcgtcaat ggttggcaca atccgttgct gattcgcatg gcagaaccgc
     1681 tcatcggttt gatgcgcaaa ctgtctgggc gctctgagaa gaaaaagaag gaatggttga
     1741 gcgaagagga ccccgctggg acgttcgttc agaagatgac cccggaatgg ctcaaaaaag
     1801 aaatcggctc gaagatgacc attgagatca agccatggcg cagtatgagc acccgcatta
     1861 tgcgctggtt cgtgcgtcct ttcggcggac gggctttctt gaggtttgtt ttctggttgg
     1921 aaggtgtctt cccgaagttc ttcgccgaaa atggacagta tccgttgatt gtggttcgta
     1981 aatcgtaatt cgtaatacgt gagtcgaaaa tagtaaatcg gcaatcgtac atgaagaaaa
     2041 aaaacttccc tgaaatcctc tcacttctgc ttatttctgc cattgtgtat ttgccgcaca
     2101 ttggtagttt gacctattac aaagacgact ggtattacat ctacgatggc atgttgggtg
     2161 gggcgaagat tttccatgaa atgttccgca tcgaccgccc ggcgcgcgga tttttctttg
     2221 agtggtatta caccctcttt ggaccgaatc cgctgccgtg gcatttgagt gtctttgttt
     2281 ggcgtgcttt agctgccctt ggcgcattgt ggatattcaa ccttctttgg aaagaaaatc
     2341 gaaaatttaa cttcattgcc gcgcttttat ttgcgattta ccctggctac ttctggtgga
     2401 tcagcgccat tgaataccag cctatgatcg cgagtttggc attacaggtc ttttcgattg
     2461 cgttcacgtt gaaggcgata cagtctgcga agattgctga aaaagctgct tatggtattg
     2521 gctcgatcct gactggttgg agttacattg ctcttgttga ctacgccatt ggcatggaag
     2581 cctttcgctt tattgctgta tatcttttga ttgcacatga catcaacgac acattttgga
     2641 aacgcttgct tgccgcgctc aaagcttggg cgtggacagt tcttatccca ttctgcattg
     2701 tcgtttggcg tatcttcttt tttaccaatg agcgcaaagc cacagatatt ggcggacagt
     2761 taagtgtttt cttttctaac ccggtctccg tcgggatcga ctggttgctt caaatctata
     2821 acagtgtttt gaatctcagc gtccaagcat ggattaatca gttccccact tttattcagt
     2881 cgatgcgttt gcgtgatatc acattgagta ttgtgcttgc tgtgatcgtg actttgcttg
     2941 tggtctttgc cgagaagcag atcaagcaat cagcggagac caaagatgaa ggtggcaaac
     3001 aagctaaagt cacgaatgag gcgctaattc tgggcgggct gggaatgatt tttggtgtcc
     3061 tcccggttgt gatggcgaat cgttatgtca acatttccgg tttttcacat tatggtttgc
     3121 ctgcatcttt ggcggcagct gtttttatgg ctggcattat tgagattatt gcgctgtcat
     3181 ctgtgcgcaa agcagtgtta tatggggtga tcgtgtttgc gtcgttatct cattttggaa
     3241 ttgcgaccaa tgcagcgatg gaagaaaaag cgctacagga attctggtgg caggtgagtt
     3301 ggcgtgtgcc ggatattcaa cctggtacga cacttgtgat ctattatccg ttgcccggca
     3361 tgggcgatgg cggcgcggca ttgatggaag ccgcgaatgt gatttatttc cctgagttca
     3421 cgggtgatgc tattgtgcat tatccgcttt cggggctcac cttgaatagc gaaaacctgc
     3481 ccggtatttt agatggaacg cagattgttg agtctgggta tcgcagccat acttatactt
     3541 ttgattacac caatatattg gtaatgagtc agcctacgtt gaattcatgt gtacatgtga
     3601 tgaatgcaag tgcgccgttg atctccgaat acgacccagt ggatgtttca ttggttgctt
     3661 cacaatcgaa gatcgaaaat gtaatagcag atgcagaacc ggtctcgccg cctgaatata
     3721 tttatggcaa agaaccggat actggctggt gtagttattt ccaagcagcg gaccttgcgg
     3781 ctcaaaccgg ggactgggag cgggttgctg ctctcggaga tgaagctatc gctttgagct
     3841 ttgcccccga agaccgcgtg gaatggttac cttttttgaa agcatatgcc attcgtggtg
     3901 atgcagaaac attgaaacaa ttatccaaac gcgtgattgg cgagaagatt atccgcttac
     3961 aggcgtgtga aatgttgagc agcattgagc aaccattaaa cgatgatgtg cgtgaaatga
     4021 tcgacataaa ctattgcaaa ggttctgagt gatgtccaaa cttacggaca aggaatatct
     4081 gacacagaag caatataaag actctacgaa cttggatgtt cgggtcgcga tccataaacg
     4141 atttagtaca aattcgtatg gatggtttaa ttgggtattt gattctttta gcgctttgcc
     4201 cgagaatgct accattttgg aaataggttg tggcacaggt gaactttgga aagagtgtga
     4261 aagccgcatc cccaaaaatt gggcgctcac gttaacggat atttctgatg gaatgttgga
     4321 tgcagcatgg cgtaatttga tccgcatcca tcgtggtatc aaatttgaaa aagtggattc
     4381 gcaatccatt ccgtatgcag ataaaacctt tgatgcggtg attgccaatt acatgctcta
     4441 tcatgtcgca gaccgtaaaa aagcatttgc tgaaattaaa cgtgttctga aagatgatgg
     4501 cgtgctattt tctgcaactg caggtgcaaa ccatttgcga gaaatgtatg agtgggtttt
     4561 tcgtgtgagt ggcgggcgac atgggcagat tgcattgcaa ttcacactcg aaaatggaaa
     4621 agaacaattg caagagttct ttccacgtgt agaattatct cgttatccag atagtttgca
     4681 gattacggat gttgatatgc tcatagcata tgtccgctcg atggcttctg tggaactttc
     4741 ggaagaagaa atacaaaaac ttgaacaaga gtggtctgaa attttagtaa aagatggcgc
     4801 aattcatatt tctaaagatg ccgggctatt tcaggctcta caatagaatc gttaatgggc
     4861 aagttgctta ataatttaga ataacaggtc ttagcaggtt gtaagcctgt tttaccgaaa
     4921 caaggagatt tcatggactg gaaaaatcaa gtagtgctcg ttaccggtgg gactggttcc
     4981 ttcggcaaga aattcacccg aattttgctt gaagagaaac aaccaaagaa gatcatcatc
     5041 tttagccgcg atgagctgaa acagcacgaa atgcaggtag gcggatacaa tgacccgcgt
     5101 ctacgctatt tcattggcga tgtgcgtgat cgcgaacgca tggtgcgcgc catgcatggt
     5161 gttgatattg tcgtccatgc ggcggcgctt aagcaggtgc ctgcttgtga gtacaacccg
     5221 atggaagcca tcaa
//
LOCUS       DAOREF010000072         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_8821_length_5234_cov_4.724464, whole genome shotgun sequence.
ACCESSION   DAOREF010000072 DAOREF010000000
VERSION     DAOREF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8821_length_5234_cov_4.724464"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKI33_02325"
     CDS             complement(<1..497)
                     /locus_tag="PKI33_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35863.1"
                     /translation="MRNLLLAIFILALASMACNMPGSAGSPVNPPIEIPTFTATVPSV
                     VSDKPTDLPAPATSTPEQAVLQPQVNTFDPRSIADCDIFVDSDFPNTIGALPDTKKAL
                     PETDKKNCQYNFANGTLFVSIFTGMPGMEAFENVRQFDALTGATISPYPIGEAAVFKD
                     FGDGH"
     gene            complement(600..3881)
                     /locus_tag="PKI33_02330"
     CDS             complement(600..3881)
                     /locus_tag="PKI33_02330"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM35864.1"
                     /translation="MAFSLWADEDEASSLANLRRHLYLLKKSLPKALQPSLIISTQTV
                     LFQFPANTWVDVLAFEQEPISLQEMEEHALFYTTDLAQGAETDEFIFSRREELRNRYA
                     QLLRMLAQTYFNYAEYETSMYWVRKLIAQDPWDEEALRLYMTSDFILGNRTTALSTYK
                     SFEKKLRLEMDIQPMPETMALYRDILHNHLLRPVSSTKTEGETFISRLSEIQTLGNAI
                     QAHGQYGNFVIINGTAGVGKTTLVREALRRFLNSSKAKLLWGDCQPAGEEQPYSIWQQ
                     IFNAAVPLLARQQDVAQEWLNHLLPLIPDLGLLRADLFSPTRPDATELRTAMRQTLFA
                     LSESRPLIIVIEDAHWMDVPSLEFLRELAESSLNIPVLFILTHRTDDAALPLFEIKRI
                     LRRKRNVVDISLQPFTTTETNQYLEKFLGTKTLPKELIDDLMQYSGGLPLLLHEVTQT
                     LNSSKRILSLREAFLFRLDRLSPAAREMVETAAVIGFSLTDIELRTALGWAAELYAST
                     MDVLQAERFLLENPSNSADEYAFSHRLIHEIIVEQISVERISNLHIRVARALEQIHSE
                     DIGFAAEIALHYQQAGQLILAAGFWVRHASESTDLAAFDSGLAAINQAEAILTGASLD
                     AQEVHAKLALQRGVIVFYQGKSEEAFSLLEKAVEHSKLFPPLYAHALSMQAYAFHTHD
                     RNELAFQAASQAFDLSMATKDVSNAVRALNIRAVSALMLGKTRGALDDLQQALALLEQ
                     HNLSASTQTVQSLYHLGTALVFSQDYQQARAILSRAVELSKKGGLRRLEAAALTMLGQ
                     IALNCGQYKLAVEIYDRSIEVAGESYLPGMWGKFAGRGWANLRMGNLLAARTDFERGL
                     DVSIQVESQYGTILMRSYLAFTALAAGESPSASFADLEADALTQALHPVVLFNANIQS
                     QGWRLFGDSEKANSAHHRAIEAAKNSDVPSFVQSARLQKLYTQVILEENIDPTYMEEE
                     LAELAKLTLDSGEVPLQVNTFLVRAWLHSRMGNLQEALSTATQALTLARACPDLPWIG
                     ECQVLLAQLFTKLGDQNQAKICTEEVHALAKTSFAPLTVEFGGEPSALVQEHLLKSLT
                     PTHLN"
     gene            4147..>5234
                     /locus_tag="PKI33_02335"
     CDS             4147..>5234
                     /locus_tag="PKI33_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNM35865.1"
                     /translation="MSQSPTIKIYLLGSVHLERGKETLEFARQKTLLLLAYLALHPQE
                     HPRERIASIFWKDATTDEAARLSLRVTINDLRKTLGEDSLIGGRDSLQLNPDLNLWID
                     VREFQRLTDSRATPEDWQSALSLYTGDLLPQVYEDWVLEWRAEFEQACLETRLRLAQH
                     QRGQGQYVAAIETARLALEIEPVRESTHQLIILCYESLGDREAALLQYESCSKALREI
                     GVEPSPELQGIYRRLQKTPASSHIAGNLPRPLTSFIGREEELNEVETLLAETRFLTLL
                     GAGGSGKTRLAIQAADESAHNYPDGVWWVDLAPLLKGDQLPQAIAKSLGVKEKLGADM
                     LDHIASFIGERKMLIILDNCEHLVSATA"
BASE COUNT         1427 a         1201 c         1227 g         1379 t
ORIGIN      
        1 atatgtccat caccaaaatc tttaaatacg gcggcttcac cgattgggta tgggcttatt
       61 gttgcacctg tcagagcgtc aaactgacgg acattctcaa aggcttccat ccccggcatg
      121 ccggtgaata tggaaacaaa gagtgtgcca tttgcaaaat tgtattggca gtttttttta
      181 tcagtttcgg gaagagcttt cttcgtatct ggaagagcgc caatggtgtt tggaaaatcg
      241 gagtcaacaa aaatatcgca gtcagctata ctgcgcgggt caaatgtgtt gacttgcggt
      301 tgcaatacag cttgctctgg agtggatgtt gcaggggcgg gcaagtcagt tggtttatct
      361 gacactacac tgggaaccgt agctgtaaaa gtaggaatct caatgggcgg gttgacaggc
      421 gagccggctg agccaggcat attacaagcc atcgaagcca gagccaaaat aaaaatagcc
      481 aatagcaggt ttcgcatgct tttctctcct ttttgcccca tatcttacag agagagcgtc
      541 ctaaaaacgt cctctaaaca agtgtttccc cttaccttca agagtttagt acggaaggac
      601 tagttcaaat gtgtgggtgt aagacttttc aataaatgct cttgcacaag cgctgaaggc
      661 tcacctccaa attccactgt taaaggtgca aaggaagttt tcgccaaggc atgcacttct
      721 tctgtacata tctttgcctg attttgatcc ccaagttttg tgaaaagctg ggcaagtaag
      781 acttggcact ctcctatcca tggcaggtct gggcaggcgc gcgccaaggt tagtgcttgg
      841 gtcgcagtgg aaagcgcttc ttgtaaattg cccatacgag aatgcaacca cgcccgcacc
      901 aaaaatgtgt tgacttgtaa tggcacttcg ccagaatcaa gagtcagctt ggctaattca
      961 gcgagttcct cttccatgta agttgggtca atgttctcct caaggatgac ttgcgtatat
     1021 agtttttgca atcttgctga ttgaacaaaa gaaggaacgt cactattttt ggcagcctcg
     1081 atcgcgcggt ggtgtgccga atttgctttt tcgctatccc caaataagcg ccacccctga
     1141 gattggatat tggcattaaa aagcacaacg ggatgtaagg cttgggtcag cgcatccgct
     1201 tcgaggtccg caaatgaagc ggaaggagat tctccagctg caagtgcagt gaacgccaag
     1261 taagaccgca tcaatattgt tccatattgg ctttctactt gaatagaaac atctaacccg
     1321 cgttcaaagt ctgtgcgagc tgccagtaga tttcccatcc gtaaatttgc ccaaccccgc
     1381 ccggcaaatt taccccacat accaggcagg tacgactctc cagcaacttc aatggatcga
     1441 tcgtaaattt caacagcaag tttgtattgg ccacagttca aagcgatctg acccaacata
     1501 gtcaacgcag ccgcttctag acgacgcagt ccgccctttt ttgaaagttc cactgcacgg
     1561 gaaagtatgg ctcgtgcttg ttgataatcc tgtgaaaaga caagagcagt gccgagatga
     1621 tacaaacttt gtacagtttg cgttgatgca gaaaggttgt gttgttccag taaggcaagt
     1681 gcttgctgaa gatcatccaa cgcgccacgt gtctttccca gcatcaaggc actcactgcg
     1741 cgaatgttta aagcacgcac cgcattcgaa acatcttttg ttgccatgga aaggtcaaat
     1801 gcttgcgaag cagcttgaaa tgcaagttca ttgcggtcgt gggtgtgaaa agcataggct
     1861 tgcatcgaaa gagcatgtgc atatagtggc gggaatagtt ttgaatgttc cactgctttt
     1921 tcaagtaaag aaaaagcctc ttcgcttttt ccttggtaaa acacgatcac cccgcgttga
     1981 agagcaagtt tagcgtgcac ctcttgagca tctaaacttg cgccagtaag aatggcttcg
     2041 gcttggttga tggcagcgag accagaatca aatgcagcca ggtctgtact ttcgctagca
     2101 tggcgcaccc agaagccggc agccaagata agctgaccgg cttgctgata atgaagggcg
     2161 atctctgccg caaacccaat atcttctgaa tggatttgtt ccaatgcgcg tgccacacga
     2221 atatgtaaat ttgaaattcg ctctacagag atctgctcta caataatttc atgaatcagg
     2281 cggtgggaaa aagcatattc gtctgcacta tttgaaggat tttccaaaag aaaccgttct
     2341 gcttgcaaaa catccattgt ggaggcgtaa agttccgctg cccaacccag agccgtacgc
     2401 aattcaatgt cggtcaggga aaatccaatc acagcagccg tctctaccat ttcacgtgca
     2461 gccggtgaga gacgatctaa ccgaaagaga aacgcctcgc gcaaagaaag gattctcttg
     2521 cttgagttga gggtttgggt cacctcatgc aataataaag gcagtccacc tgagtattgc
     2581 atgaggtcat ctataagttc ttttggaaga gtctttgttc ccaaaaattt ttcaagatat
     2641 tgatttgtct ccgttgttgt aaatggttgc agagagatat ctactacatt tcgcttacgg
     2701 cgtaatatcc gtttgatttc aaacaaaggc aatgcagcat catctgtgcg atgggtaaga
     2761 ataaaaagaa caggaatgtt tagagagctt tcagcaagct ctcttaagaa ctctaaggat
     2821 gggacatcca tccaatgtgc atcttcaatc actatgataa gcggacggct ctctgacaag
     2881 gcaaataaag tttgcctcat agcggtgcgc aattcggtag catctggacg ggtaggggaa
     2941 aacagatcgg ctcttaacaa gcccaagtct gggatgagcg gcagtaaatg atttagccac
     3001 tcctgagcca catcctgttg acgcgcaagc aaagggactg cggcattaaa aatttgctgc
     3061 caaatcgagt aaggttgttc ttcccccgca ggctggcaat ctccccaaag tagtttggct
     3121 tttgatgaat ttaaaaatcg ccgaagcgct tcacggacca aagtggtctt tcccacaccg
     3181 gctgttccat tgatgatcac aaaattgccg tattgcccat gtgcctggat cgcatttccc
     3241 aatgtttgta tttcacttaa ccggctaatg aatgtttcac cttctgtttt tgtggaagaa
     3301 acaggacgta aaaggtggtt atgcaaaata tctcggtata gtgccatggt ttctggcatg
     3361 ggctggatgt ccatttccag cctcaacttt ttctcaaatg atttataagt tgaaagagca
     3421 gttgtgcgat taccaagaat gaagtctgaa gtcatataca accgcagggc ttcttcatcc
     3481 catgggtctt gagcaatcag ttttcgcacc caatacatag atgtctcgta ttcggcgtag
     3541 ttgaagtatg tctgtgccag cattctcaaa agctgtgcat atcgattacg caattcttcg
     3601 cgacgagaaa aaatgaactc atctgtttct gccccttgtg caaggtctgt tgtgtaaaac
     3661 agggcgtgtt cttccatttc ttgcaaagaa atcggttcct gttcaaacgc aagcacatcc
     3721 acccaggtgt tagcagggaa ttgaaataaa accgtttgtg tagagatgat caatgatggt
     3781 tgtagggctt ttggaagaga ttttttcagg aggtaaagat gccgccttaa attcgcaaga
     3841 gaactggctt catcctcatc tgcccagagt gagaatgcca aatgtttccg cccatgtggg
     3901 gaattcgcct gaaggacaag tcgggtgaat agacatagta atttatcgcg tgcaggcagg
     3961 gcagatataa cgccattagc ctcaatccta ggtgatccaa ataaaaaaat gcgaatcctg
     4021 tgtgcctgcg acatgaagaa attataacga ttcacattaa aagatttgga atggcgaccc
     4081 tattaagggg gcatggtaca ctctgtaaaa aacacatctt aataatcttt gatttcctct
     4141 tttaatatgt cacagtcacc tacaatcaaa atttacttgc ttgggtcagt ccaccttgaa
     4201 cgcgggaagg aaacacttga gttcgcgcgc caaaaaactt tactcttatt ggcatatctt
     4261 gccctccacc cgcaagagca tccgcgcgaa cggattgcct ccatattttg gaaagatgct
     4321 actaccgacg aagctgccag gctttcgctt cgtgtgacga tcaacgactt gcgtaagact
     4381 ctcggcgaag atagcctaat tggcggtcgc gactcgttgc aacttaaccc tgacttaaac
     4441 ctatggattg atgtacgcga gtttcaacgt ctaactgact cgcgtgctac gccagaagat
     4501 tggcaatctg cactgagttt atatactgga gatctacttc cacaagttta tgaagattgg
     4561 gtattggaat ggcgggcaga atttgaacaa gcttgccttg aaacccgttt acgccttgct
     4621 caacatcaaa gaggtcaggg tcagtatgtg gctgcaattg aaaccgctcg ccttgcgcta
     4681 gagattgaac ccgtccgtga atccacgcat caattgatca tcctctgtta tgagtcactt
     4741 ggagatcggg aagcagcgct cttacaatac gaatcgtgca gtaaagctct acgagaaatt
     4801 ggcgtagagc catcgccaga gttacaaggt atttatcgcc gcctgcaaaa gacacctgca
     4861 tcttcccata tagcaggcaa cctcccccgc ccattgacca gctttattgg gcgcgaagaa
     4921 gaactgaatg aagtagaaac cttattagca gaaactcgct tccttacctt attgggcgca
     4981 ggtggaagtg gcaagacccg gcttgccatt caagcagctg atgagtctgc acataactat
     5041 cctgacgggg tctggtgggt tgaccttgct cccctgctca aaggagacca attgccgcaa
     5101 gccattgcga aatcgcttgg ggtaaaagaa aagctcggcg ccgatatgct tgaccacatt
     5161 gcaagtttta tcggcgaacg caagatgcta atcatcttgg ataactgtga acatcttgtc
     5221 tccgctactg ctca
//
LOCUS       DAOREF010000073         5153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9037_length_5153_cov_5.037858, whole genome shotgun sequence.
ACCESSION   DAOREF010000073 DAOREF010000000
VERSION     DAOREF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9037_length_5153_cov_5.037858"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PKI33_02340"
     CDS             <1..163
                     /locus_tag="PKI33_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNM35866.1"
                     /translation="HNHPSGDPTPSPDDVAVTRAIVQAGKLLDVDVLDHLVIGQAKWV
                     SLKERGLGF"
     gene            complement(160..1785)
                     /locus_tag="PKI33_02345"
     CDS             complement(160..1785)
                     /locus_tag="PKI33_02345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM35867.1"
                     /translation="MFSNPYSPYLFTSAITSFVMAMLLWRRRIAPGATPLSVLLGLTS
                     VWALASALLLESSSTLLRELWFDILSISAMMTTPMLLIFCLEYTERQNFLSKRLLLFV
                     YLFPILNAILCVTTNYHHLFYANVDFSYQSANFLALVPGPFYWIYTVYSYGIALFIFV
                     LILTNFFQTSSTYRKQTATILLGLLFPFVGNVIYTLLLGVGKGAFDPTPMLFTVMGFF
                     LVCGLLWFQLFDVIPVARHTLVEHMQDGVIVVDVKNRIVDVNPSALKWLNWEGKSSPI
                     GKNVEELLTSWFEQFANLPIDLYVQTEVHKKGTPGLYFDLRVEPIYNRQNVLTGRLIV
                     FRDITRQKMSEKALLEAHDRLRLHLKEIELLQSELREQVIRDPLTGLFNRRYMEETLE
                     REISRAIRENISIGVCMADIDQFKSFNDEHGHKAGDLILKKLSEILTNYSRAEDVVCR
                     YGGEEFLILMPGAELDAIARRAEDWRRAFEQAKVEFDGKQLSTTLSMGIAMFPQQART
                     SDDIVKLADEAMYLSKHNGRNQVSIAQTWGEKR"
     gene            complement(1789..2091)
                     /locus_tag="PKI33_02350"
     CDS             complement(1789..2091)
                     /locus_tag="PKI33_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNM35868.1"
                     /translation="MQPNPNEPVRVHIWVKGRVQGVGFRAHVEYSARQIGGLSGWVRN
                     VGDDTVEAIAEGQREQVERLIEAMKQGPRMSRVDEAKIDWESPTGEYNGFRVKSSA"
     gene            complement(2072..3304)
                     /locus_tag="PKI33_02355"
     CDS             complement(2072..3304)
                     /locus_tag="PKI33_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNM35869.1"
                     /translation="MSATRQRILQVFMVSLLVATLSLKPNQAQAQEGIVVENPNATPD
                     FGKTITFTAKIKASNPIQQASLLFRGVNEETTRVETLQVAEDGSVQFIYDASQNVFPP
                     FSWVVYWFQATLSDGKTYTSESNRVQYVDNRFPWRDVTQANVTIHWYAGDDAFGASAL
                     DSAGAGMLGMKDFIPLSLTEPIHIYIYSNVQDLGDTLMLGGREWAGGHAHADLGVVLV
                     AIAPGANQFTEMESLIPHELAHVMLYRALGDTYYKQPAWLIEGIASMVELYPNPDYAR
                     ALEIASVNDPLIPFSDMCASFPADSGSAFLAYAQSQSFVTYIRDSFGVSGLSRLISTY
                     GDGFDCDLGITKALGASLNQLDSRWRENVLGQNQVGAALRNLLPFLLLLLLVLIVPIW
                     GAVDVMRRRRKKYAAKSK"
     gene            complement(3310..4227)
                     /locus_tag="PKI33_02360"
     CDS             complement(3310..4227)
                     /locus_tag="PKI33_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNM35870.1"
                     /translation="MSFIELFNTFANNLLPIMLIAAAGYILGKTLTVDSRTIGRMVFY
                     IFSPLLVFNLMVTSQLNLKQALTTVGYTVAVIAIMGLLAFIFGKIFQLSRPHLLAVII
                     TVAFGNTGNYGLPLVKFAFGDEALAVASIFYVTTTILFNTVGVVIASLGHLDLKSAIL
                     GVFKLPILYAVTIALILKGFGIAIPLPLSRTIEIAANGAIPAMIILLGLELTRIEWTQ
                     SLRATGVGVAAKLLIGPLVGLLLASLFGLQGHTRQGSVIEAAMPAAVATTVVATEYKL
                     EPSLVTAIVFFGTILSPITLTPLIVFLTR"
     gene            complement(4289..>5153)
                     /locus_tag="PKI33_02365"
     CDS             complement(4289..>5153)
                     /locus_tag="PKI33_02365"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNM35871.1"
                     /translation="HACTFCIIPYKRGIERSRPVGDIVSEVRSLAKQGVKEITLLGQI
                     VDRYGKDIPDGPKLADLLRIIHEVEGIERIRFLTSHPNYFDDDLINAIAELPRVMPHI
                     ELPIQAGDDTVLENMKRGYTQQQFRDLVAKIRQHIPNCSIATDIIVGFPGETEEQFME
                     TYRVLSDLKLDVAHLARYSIREGTVATRRMVDDVPEEEKMRRLHMLDDLQEQIVGEIN
                     KKYLGQTVDVLFEENVKGRWKGRTPTNKLVFVQSEEDLRGKLLPVTVTWTGPWSMQAN
                     LVKQPAELISL"
BASE COUNT         1362 a         1212 c         1295 g         1284 t
ORIGIN      
        1 acataatcat ccgagcggag accccactcc cagcccagat gatgtagctg tgacacgtgc
       61 cattgtacaa gccgggaaac tattggatgt ggatgtgctc gaccatttgg tgattggtca
      121 ggcaaagtgg gtttcactga aagagcgcgg tttgggtttc tagcgttttt cgccccatgt
      181 ttgtgcaata cttacttgat ttctgccatt gtgtttagaa agatacatgg cttcatctgc
      241 taacttgacg atgtcatcac ttgtccgcgc ttgttgaggg aacatagcaa tgcccatgga
      301 taacgtggta gagagttgct tcccatcaaa ttctactttt gcttgctcaa atgctctgcg
      361 ccaatcttcg gcgcggcggg caatggcatc caactccgcg cccggcatga ggattaagaa
      421 ctcttcgcct ccatatcgac agacgacatc ttcggcgcgc gagtaattgg ttaatatctc
      481 tgatagcttt ttcaagatca agtcaccagc tttatgacca tgctcgtcat tgaaagactt
      541 gaactggtca atatctgcca tacaaacccc gattgaaata ttctcgcgaa tggctcgcga
      601 gatttctctc tccaatgttt cttccatgta acgacggttg aacaaccctg tcagcgggtc
      661 gcggatgact tgttcgcgca actcactttg cagaagctca atctctttca gatggagtct
      721 taagcggtca tgggcttcca ataaagcctt ttcagacatt ttctggcggg tgatatcccg
      781 aaacacgatc agccgacccg tgagaacgtt ttgcctattg tagataggct ctacacgcag
      841 gtcaaaatac aaccctggag tgcctttttt atgtacttct gtttgaacat aaaggtcgat
      901 gggtaagttt gcgaattgtt caaaccacga agtaagaagt tcttcaacat ttttcccaat
      961 gggggatgat ttgccttccc aattcagcca tttcaaggcg gatggattca cgtctacgat
     1021 gcgatttttt acatccacta caatcacgcc atcttgcatg tgttcaacca gtgtatgccg
     1081 cgcaactggg atcacatcga ataactgaaa ccagagcaac ccacatacaa ggaagaatcc
     1141 catcacagta aacaacatag gtgtggggtc aaaagcacct ttgccaaccc caagaagcag
     1201 agtgtaaatc acgttcccaa cgaatgggaa caatagccca agtagaatag tggctgtttg
     1261 cttgcgataa gttgaagagg tttgaaaaaa attcgtaagg ataagaacaa atataaaaag
     1321 cgctatcccg taggaataca cggtgtatat ccaataaaat ggacccggaa ccaaagcaag
     1381 aaaattagca gactggtatg aaaaatcaac atttgcatag aacaggtggt ggtaattcgt
     1441 tgtgacacac agaatggcat taagaatcgg aaataaatag acaaagagta acaatctttt
     1501 acttaagaag ttttggcgct ctgtgtattc caaacaaaag ataagcagca taggggtggt
     1561 catcattgcg cttatgctta atatatcgaa ccataactcc ctgagtaacg tactggacga
     1621 ctctagcaac aaagccgatg ccaacgccca aactgaagtg agacccaaga ggacggataa
     1681 tggagtcgcc cccggagcta tgcgtcgacg ccacagcaac attgccatca caaatgaggt
     1741 gatggctgag gtaaataggt agggggagta tgggtttgaa aacataactt atgcgctgct
     1801 ttttactcta aacccattgt actcccctgt gggcgattcc caatcgattt ttgcctcatc
     1861 cacccgtgac atgcgcggac cttgtttcat cgcctcgatc aaacgttcaa cctgttctcg
     1921 ttgaccttca gctatggctt ccactgtatc atcccctaca ttgcgcaccc agccacttaa
     1981 ccctccaatt tggcgcgccg aatattctac gtgtgcacga aacccaaccc cttgcacccg
     2041 tcccttcacc caaatgtgta cacggactgg ttcatttgga tttggctgca tatttctttc
     2101 tccttcggcg catcacatca acggctcccc agattggtac gatcaaaaca agaagaagta
     2161 aaagtaggaa gggcaataag tttcgtaacg ctgcgcctac ttgattttgc ccgagcacat
     2221 tttcacgcca acgtgaatcc aattggttga gcgaagcacc taaggctttg gtaatgccga
     2281 ggtcacagtc gaagccgtca ccgtaagtac taatcaggcg tgagagaccc gagaccccga
     2341 acgagtcgcg gatgtaggtg acaaaggatt gagattgtgc atatgccaga aatgctgacc
     2401 cagaatcggc ggggaaagag gcgcacatat ccgaaaatgg aatcaaggga tcattcacac
     2461 tggcaatttc caaagcacgc gcataatcgg ggttggggta aagctccacc atggaggcaa
     2521 tgccttcaat cagccaggca ggttgtttgt agtaagtatc gccaagcgcg cgatatagca
     2581 tgacgtgtgc aagttcatgc gggattagag attccatttc ggtgaattga ttggcgccgg
     2641 gcgcaattgc aaccaatacc acgccaaggt ctgcatgagc gtgtccgcct gcccattcgc
     2701 gcccgccgag catcagcgta tcgccaaggt cttggacgtt ggagtagata taaatatgaa
     2761 tgggttcggt aagtgagagc ggaataaaat ctttcatccc caacatgcct gcaccggcac
     2821 tatctaatgc gcttgcacca aaagcatcat cgccggcata ccaatgaatg gtgacgttgg
     2881 cttgcgtaac atctctccat gggaatcgat tatcaacata ttgaacgcga tttgattcgc
     2941 tggtgtaggt tttgccgtca cttaatgttg cttgaaacca atagacgacc caactgaatg
     3001 gagggaaaac attttgcgaa gcgtcataga tgaattgtac cgagccatct tctgcaactt
     3061 gtaaggtttc tacacgggtc gtttcttcgt tgaccccgcg gaacaagagc gatgcttgtt
     3121 gaataggatt ggatgctttg atctttgctg tgaatgtgat cgtttttccg aaatcggggg
     3181 tcgcatttgg attctcgaca acgattcctt cttgcgcctg ggcttggttc ggtttcagag
     3241 aaagggtcgc cacgagaagt gagaccatga agacttgcaa aatcctttga cgtgtggcgg
     3301 acatgttcat tacctcgtaa ggaaaacgat caacggtgta agggtaatcg gactcagtat
     3361 ggtgccgaag aaaacaatcg ctgtcacaag agacggttca agtttgtatt cggtagcaac
     3421 aaccgttgtt gctaccgcgg cgggcatggc ggcttcgatg acactgccct gacgggtatg
     3481 cccttgcaac ccgaacaggc ttgccagcaa taacccaacc aacggtccga tcaataactt
     3541 agcagcaacg cccacgccag tcgcgcgcag gctttgcgtc cattcgatgc gggtgagctc
     3601 caagccgagc aagatgatca tggcaggaat ggctccgttt gcggcgatct cgatggtgcg
     3661 tgacagcggc agtggaatgg cgatgccaaa gcctttgagg attaatgcaa tggtcacagc
     3721 gtaaagaata ggcagcttga atactcccag tatagcggat ttcaaatcta aatgcccgag
     3781 cgatgcaatg accaccccaa cggtattgaa aagaatggtg gtcgttacat aaaaaataga
     3841 agcaactgcc aatgcttcat cgccaaatgc gaatttaacc aagggcaagc catagttgcc
     3901 cgtgtttcca aatgcgaccg tgataatgac cgccaagagg tgtgggcggc taagttgaaa
     3961 gattttcccg aagatgaaag caagcagccc cataattgca atcacggcaa ctgtgtatcc
     4021 cactgtagtg agggcttgct tcaggtttaa ctggctggtg accattaagt tgaaaacgag
     4081 taaggggcta aaaatgtaaa agaccatacg cccaatggtg cgcgagtcga cggtgagcgt
     4141 tttcccgagg atgtatccag ccgcggcgat gagcatgatg ggcagcaagt tgttggcaaa
     4201 ggtgttgaac agttcgatga aggacatgtt gaatgaaatt ataacccccg aaaaaggtag
     4261 gggcggatcg cgatccgccc ctacaaaatt acaacgaaat caattcagcc ggttgtttta
     4321 ccaaattcgc ctgcattgac cacggacccg tccaagtgac ggtgacgggt agaagtttgc
     4381 cgcgtaggtc ttcttccgat tgcacaaaga ccagtttgtt ggttggcgtg cgccctttcc
     4441 agcgaccttt gacgttttct tcgaagagta catctacggt ttgaccaagg tacttcttgt
     4501 tgatttcacc gacaatctgt tcttgcaaat cgtcgagcat atgcaggcgg cgcatctttt
     4561 cttcttctgg tacatcgtct accatgcggc gagtggcaac ggtcccttcg cggatggagt
     4621 agcgggctaa atgggctaca tccaacttga ggtccgataa gacacggtag gtttccatga
     4681 actgttcctc ggtctcgccg gggaatccaa cgatgatatc ggttgcgatg gaacagttcg
     4741 ggatgtgctg gcggatcttt gccacaaggt cacggaattg ttgttgggtg tatccgcgtt
     4801 tcatgttttc gagcacggtg tcgtcgccag cttgaatggg aagctcgata tgcggcataa
     4861 cgcgcggtaa ttcggcaatg gcgttgatga ggtcatcatc aaagtagttg gggtgtgagg
     4921 ttaagaagcg gatgcgttca atgccttcta cttcatggat gatgcgaagc aggtctgcaa
     4981 gtttgggacc atctggaatg tctttcccgt agcggtctac gatctgaccg agcaatgtga
     5041 tttcttttac gccttgcttt gccaacgagc ggacttcgct gacgatgtcg ccaactgggc
     5101 gagagcgttc gataccgcgc ttgtagggaa taatgcagaa ggtgcaggca tgc
//
LOCUS       DAOREF010000074         5142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9067_length_5142_cov_3.907214, whole genome shotgun sequence.
ACCESSION   DAOREF010000074 DAOREF010000000
VERSION     DAOREF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_5142_cov_3.907214"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKI33_02370"
     CDS             <1..478
                     /locus_tag="PKI33_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM35872.1"
                     /translation="GFGMRILYYSPNSEPEFGAIKMNSLDEVLHESDFVSVHTPLNES
                     TRHMVNADFLSKMKPNAVFVNTSRGPVVDQVALYEALKAKKIFAAGLDVTDPEPLPLD
                     SPLLTLDNCVIVPHIGSASERTRDAMARLAAENLIAGLKGERLPHCINPEVYENRR"
     gene            514..1833
                     /locus_tag="PKI33_02375"
     CDS             514..1833
                     /locus_tag="PKI33_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNM35873.1"
                     /translation="MNRTKDARVCIIGAGPSGITAGKNLIQAGVTNFVIYEKNDQVGG
                     NWVFNDKTKHSSVYETTHLISSRRYSEYFDYPMPADYPDYPSHTQMLAYFQGYAKHFG
                     VMPYIQFNTEVHNAEELPDKTWRITLGNSKVEIFDFLLIANGHHWNPRYPKYEGEFTG
                     EFVHSHEYKRAAPFAGKRVLVIGAGNSGCDISVETARVSAFTAMSMRRGQYIVPKFVF
                     GQPPDVVNGRFYWLPAWIRLPFFYLTLKLAVGKYEDYGLPKPKHGILNAHVTNNSEIL
                     YAIRHGKVHVRPDIKRFNGKQVEFVDGRVEEYDSIIAATGFKISLPFFDPNLLSIEEG
                     VPPLYKNVFHPDHPSLFFIGLVQPLGSIWPLSDLHAKLAANYIIGNYHLPKDIRQRIA
                     RHIEYMKKHYVNSPRHSVEVEFHPHIVELKREIPKSAPEWKHGYSDR"
     gene            1814..2482
                     /locus_tag="PKI33_02380"
     CDS             1814..2482
                     /locus_tag="PKI33_02380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNM35874.1"
                     /translation="MDIQIDNLVRAKRRTIALIVERDGSLTVRAPRRATLNDIYGFIQ
                     EKTDWILRSREKLKAIKPIPKKAYVDGERFLFLGQEYELRLVPPQRPALKFDGGFTLS
                     TSAQERGEQAFTKWYKTQALTVFTECVNHYANLHGLIPKEVKVNSAKTRWGSCTSAGT
                     LNFTWRLVMAPLEVIDYVVLHELAHLKVKNHSPRFWKLVESLCPDFKHHRKWLRDHGE
                     TMTL"
     gene            2503..2925
                     /locus_tag="PKI33_02385"
     CDS             2503..2925
                     /locus_tag="PKI33_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35875.1"
                     /translation="MLPETLIEVHEHTKPDYKPLVDYQSWRVALMNYTDDLTPENINR
                     MQKHTETDEVFVLMAGHCILFLGEGEETVTKVHAVDMELYKLYNVKRGVWHSHTFSKD
                     ARILIVENRDTVVENSPFVDLSPSQCTEVLELTKQLWK"
     gene            complement(2960..3808)
                     /locus_tag="PKI33_02390"
     CDS             complement(2960..3808)
                     /locus_tag="PKI33_02390"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNM35876.1"
                     /translation="MKAELFFDAKSNLGEGPSWDAKTQTLYWVDIFEKRVYAGNEILA
                     QFDEYVGFVAPTKSGHLIVGKRASFVDLDIATSQQTVLVTLNESATNRMNDGKCDPSG
                     RLIAGTMDLNETDPTGSVYSYDGKSTRVLFRDVTISNGMAWSPDYKTFYYIDTPACEV
                     RAYDYDIKTGEIANRRRAFEVPKSLGWADGMTSDVEGNLWIAMWGGAQVTRWNPNTGQ
                     LLEQVSVPALHSSCPVFGGKNRNELYITSARKGMSEADLNKYPLSGGLFKVDTKYEGM
                     PTFEFG"
     gene            complement(3808..4734)
                     /locus_tag="PKI33_02395"
     CDS             complement(3808..4734)
                     /locus_tag="PKI33_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNM35877.1"
                     /translation="MLSIYKTTENGLEKLDAIANGTWVNVIDPTPDEMEKLVGWGMEM
                     DYINYSLDQDEMARMERDEDYTFILLRIPIHQPESDIPYNTVPLGIMILGNKIITVCR
                     FDSDIFRALTNGKHRYLKTGKRYRLTLYIFLETAVRYLNLLREINRATEAVEDRLQKS
                     TQNREVLELLKYQKSLTYFATALRSNEVMMERVQKTQLFNYYEEDQDLLEDVLTENQQ
                     AIQMTSIATEILSGMMDAFASIISNNLNVVMKALAALTIILNMPTIVASFYGMNVDLP
                     GEGHPLAFLTVIGLSLALTALATYIFYKRNWF"
BASE COUNT         1395 a         1265 c         1167 g         1315 t
ORIGIN      
        1 tggttttgga atgcgtattt tgtattacag cccaaactca gaacctgaat ttggtgcgat
       61 caaaatgaac agccttgatg aggtattaca tgaatcagat tttgtttcgg tgcatacgcc
      121 gttgaatgag tcgacgcggc atatggtaaa tgcagatttt ctgtcgaaga tgaagccgaa
      181 tgcggtcttt gtcaacacct cacgcggacc ggtggtagat caagtcgctt tgtatgaggc
      241 gttgaaagca aagaaaattt tcgccgccgg gttggatgtg accgaccctg aacctttgcc
      301 cttggattct cccctactga ctttagataa ctgcgtcatc gttccgcaca tcggcagtgc
      361 cagcgaacga actcgcgatg caatggcgag acttgcggca gaaaatctca ttgccgggtt
      421 aaaaggcgag cgtttgccac attgcatcaa ccctgaagtc tacgaaaaca gacgatagac
      481 aatgggtttc catagtcaat aagaggtttt attatgaata gaacaaaaga cgcacgtgtg
      541 tgcattatcg gagcaggacc ctctggcatt acggctggga aaaatctcat tcaggcaggt
      601 gtcaccaatt tcgtcatcta tgaaaagaac gaccaagtcg gcgggaattg ggtcttcaat
      661 gacaagacca aacactcgtc tgtgtacgaa acgacgcacc tcatcagctc gcggcgttat
      721 tcggaatatt ttgattaccc catgcccgca gactatcccg attacccgtc gcacacgcag
      781 atgctggcat attttcaggg gtacgccaaa cactttggtg tgatgcctta tattcaattc
      841 aacaccgaag tccacaacgc tgaagaatta ccagataaaa cttggcgcat cacacttggg
      901 aatagcaaag tagaaatctt tgactttcta ctcatcgcga atggacatca ttggaatcca
      961 cgttatccaa agtatgaagg tgagttcacg ggagaattcg tccattcgca tgaatacaaa
     1021 cgcgcagcac catttgcagg gaagcgtgta ctcgtcattg gggcgggcaa ctcgggctgt
     1081 gacatttcgg tagaaaccgc acgtgtttcg gcgttcaccg ccatgtccat gcgacgcggg
     1141 caatacatcg tgcctaagtt cgtttttggt cagccgcccg atgtggtcaa cggtcgtttt
     1201 tactggctcc ccgcgtggat tcgcctgccc ttcttttatc taaccctcaa acttgctgtc
     1261 ggtaaatacg aagattatgg cttgcccaaa cccaaacacg gcatcttgaa cgcgcacgtg
     1321 accaacaact cagaaattct ctacgccatc cgtcacggga aagttcacgt ccgcccagat
     1381 atcaaacgct tcaacggcaa acaagtagag tttgtggatg ggcgcgtgga agaatatgac
     1441 tcgatcattg cagctaccgg atttaagatc tcgcttcctt tcttcgaccc gaacctgctc
     1501 tccatcgaag aaggtgttcc tccgctgtat aagaatgttt tccatcccga ccacccatcg
     1561 ctattcttta tcggtcttgt gcaaccgctc ggctcgatct ggccactctc agacctgcac
     1621 gccaaacttg ccgctaatta tattatcggc aattaccacc tacccaaaga tatacgtcaa
     1681 cgcattgcgc gccatattga atatatgaaa aaacattacg tcaattcgcc gcgccacagc
     1741 gtggaagtgg agtttcatcc gcacattgtg gagctaaagc gcgagattcc caagtctgcg
     1801 ccggaatgga agcatggata ttcagatcga taaccttgtg cgcgccaaac gccgcaccat
     1861 tgccctcatc gtcgaacgag atggcagcct taccgtccgt gcgccgaggc gggctacact
     1921 gaatgatatc tatggtttca tccaagaaaa aaccgattgg atcctacgtt cgcgcgaaaa
     1981 actaaaagcg ataaagccca tcccgaaaaa agcatatgtg gatggagaac gtttcctctt
     2041 tcttgggcag gagtacgaac tccggctcgt tccacctcag cggcctgccc tcaaattcga
     2101 cggtggattc accctgtcaa cttctgcaca agaacgcggt gaacaagcct tcaccaaatg
     2161 gtacaaaacc caagcattga ccgtctttac ggaatgtgtc aaccattacg ccaacctaca
     2221 cggacttatc ccgaaagagg tcaaagtcaa ctcggcaaag acacgttggg gttcttgcac
     2281 atctgccggc acgctcaact tcacctggcg cttggtcatg gcgccactcg aggtgatcga
     2341 ttatgtcgtg ctccatgagt tggcgcattt gaaggtcaaa aatcattccc cgcgattttg
     2401 gaagttggta gaatcgctct gccctgactt taaacatcac cgcaagtggt tacgcgacca
     2461 cggcgaaaca atgaccttat aacttttctc tggagaatag ccatgctgcc cgaaactctc
     2521 atcgaagtcc acgaacatac caagccagat tacaaacctc ttgtcgatta tcagtcttgg
     2581 cgagtggcac tgatgaacta tacagacgac ctaacccccg agaacatcaa tcgcatgcaa
     2641 aagcatacag aaaccgacga ggtctttgtg cttatggctg ggcattgtat tttatttctc
     2701 ggcgaaggcg aagagacggt aaccaaagtc cacgccgtgg atatggagtt gtacaaactt
     2761 tataacgtta agcgtggcgt atggcattcg cataccttca gcaaagatgc ccgcattttg
     2821 atcgtggaga atcgcgatac cgttgtggaa aattcgccct ttgtggatct atcgccaagt
     2881 caatgcacag aagtgctgga attaacaaaa caattatgga agtaaaacgt aagagcgagg
     2941 aaaccccgcc cttacgtttt tacccaaact caaaagtggg catgccctca tattttgtgt
     3001 caactttgaa caacccgcca gagagcgggt atttatttaa gtccgcttcg ctcatgcctt
     3061 tgcgagcaga ggtgatatac aactcattac ggttcttgcc accaaagacc gggcaggaag
     3121 agtgcaaagc aggcacagaa acctgctcca agagttgacc ggtattcggg ttccagcgtg
     3181 tgacctgtgc cccaccccac atggcgatcc atagattgcc ttcaacatca gaggtcatgc
     3241 cgtctgccca gccaagggat ttgggaacct caaaagcacg ccggcggttc gcgatttcac
     3301 cggtcttgat atcataatca taggcgcgga cttcgcaggc gggcgtatca atgtaataga
     3361 aggttttata gtctgggctc catgccatgc cattggagat ggtcacatca cggaacaaaa
     3421 cacgggtgga ttttccatca taggaataga cagagcctgt gggatccgtc tcgttcaaat
     3481 ccatggtgcc tgcaatcaag cgcccagatg ggtcacattt gccatcgttc atacggttgg
     3541 tggcagattc attcaaagtg acaagcacag tctgctggga ggtggcaatg tccaagtcca
     3601 cgaaagaggc gcgtttgcct acgatgagat gtccgctttt agtaggggca acaaatccaa
     3661 catattcatc aaattgagcg agaatttcgt tacctgcata gactcttttt tcaaaaatat
     3721 caacccaata caaggtttga gtttttgcat cccaagatgg accttcgccc aagtttgatt
     3781 ttgcgtcaaa aaaaagttca gctttcatta gaaccaattc cttttataga agatataagt
     3841 tgccaatgcc gtgagcgcca acgacaagcc aataaccgtt aagaatgcca gcgggtgtcc
     3901 ttctcctggc aaatccacat tcatgccgta aaacgatgca acgatggtgg gcatattgag
     3961 aataatcgtc agtgcggcaa gcgctttcat cactacgttt aagttattcg agatgatcga
     4021 agcgaaggca tccatcatac cagagagaat ttcagtagcg atgcttgtca tctgaatcgc
     4081 ttgctgattt tccgtgagca catcttcgag caagtcttga tcttcttcat aataattgaa
     4141 gagttgagtc ttttgtacgc gttccatcat cacttcgtta gagcgcaacg cggtcgcaaa
     4201 gtaggtaaga ctcttttgat acttaagcaa ttcaagcact tcgcgattct gagtagattt
     4261 ttgcaagcgg tcttccacgg cttcggtagc gcgattgatc tcacgcaaca agttcaaata
     4321 acgcacagcc gtttcgagaa agatataaag cgtgaggcga taacgcttgc cagttttcaa
     4381 atagcgatgt ttgccattgg ttaatgcgcg aaagatatca ctatcgaagc ggcaaactgt
     4441 gatgatctta ttgccaagaa tcataatgcc aagcggaacg gtgttataag gaatatcgct
     4501 ctctggttgg tgaatgggaa tgcgaagaag gatgaaggtg taatcttcat cgcgttccat
     4561 acgcgccatt tcatcttgat caagtgaata attgatatag tccatctcca tcccccaacc
     4621 tactaacttt tccatttcat ccggcgttgg gtcaatcaca ttaacccacg ttccattcgc
     4681 gatcgcatcg agcttctcta gcccgttctc ggtcgttttg tagatactca gcataacagc
     4741 ctcctacgtg gaattatcac gtaagggcat ggcacctacg tgaaaggtat taaaacgtta
     4801 aatacatgtt caccgctact tagcgggtta ctatctgaag aacctacatc gctttcagtg
     4861 gttagataca tacttttgaa ccgcgccaat tttaagccgg atgatggaat tacacaacag
     4921 tttcaggatt tggcttttag agtatgtaaa aactcgatgt gtgtcctggc tatttctttc
     4981 tttcaaggaa gatacaacct ctatggattt gggtcaacgg ctatatcttt ttcaaatgca
     5041 ccaatgcttc aaataaaacc gctacatgca gcgcatgttt gaatttgcct tgtcttacca
     5101 gctctattaa gtcatcgaac ggaatcagta caacttcaat tt
//
LOCUS       DAOREF010000075         5106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9153_length_5106_cov_4.461097, whole genome shotgun sequence.
ACCESSION   DAOREF010000075 DAOREF010000000
VERSION     DAOREF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9153_length_5106_cov_4.461097"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PKI33_02400"
     CDS             <1..1312
                     /locus_tag="PKI33_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNM35878.1"
                     /translation="QSLDELKKYVAQPSISAQNLGLKECAQLVKEMLEKRGFTAEIMA
                     TEGAPVVFAERKGNVDKTILIYNHYDVQPPEPLELWDSPPFEPEIRDGKMYGRGVSDD
                     KGHLTSRLHAIDAILAEDGELPCNIKFIIEGEEETASVHLHEFISNNVERLKADACIW
                     EFGSVDHRDRPQQYLGLRGICYVELSVTSLGTDVHSGIGGSIFPNAAWRLTWALSTLK
                     GVDERIRIPGFYDDVVQPTDRDREFMEKLPDLAEDYKTRFGVQKFIKGLEGGMDLKLE
                     EVFTPTCTICGLTSGYQGPGSKTVQPASASAKVDFRLVPNQMPKDILKKLRAHLDAEG
                     FSDVEIKFLGGEPAARTDPDHPFVKLVSETAEEVYDAKMDIVPMIGGSGPNYPFVHEL
                     GLPVVCMGIGYPNTRAHAPNENFRIDLYAQHAKHTARVIREFAK"
     gene            1488..2129
                     /locus_tag="PKI33_02405"
     CDS             1488..2129
                     /locus_tag="PKI33_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HNM35879.1"
                     /translation="MFVGERMSHPVITVTPETPVHDALAMFKKEHIRRAPVVKDGKLV
                     GIVSESDLLNASPSPVTSLSVWEMNYLISKVTVKQVMSKKLITVDVNTPIEEAARIMA
                     DKKLGGLPVMRSDKLVGIITETDLFKIFLELMGARTKALRVTAEIDDKPGTLAKLTKA
                     VSDAGGNFISFGQFSGDDTSSKILTFKVAGLKKDEVTSALSKVVKKFWDIRQS"
     gene            complement(2198..3886)
                     /locus_tag="PKI33_02410"
     CDS             complement(2198..3886)
                     /locus_tag="PKI33_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35880.1"
                     /translation="MKKYPFFGLISILVLSSLILSACGVSEDYEEDFSTEEVATEEIV
                     TEEATQASSDATSITPSAPGDVIYDFGFTPEENGFSFENYGDEIPATNLTADELRRMF
                     GDEVCADINGDECILTPPAAQWMEELNTDMSGGHCEGMAVLSLVMYTGQVSPSEFGGK
                     TASELDITDKALQREIAYWWATQAVDPTASSIISGTPMEILDLVEKMDVKGETYTIGI
                     YNENGDGHAITPFGVEDKGDGLYAILVYDNNYPGETRELFIDSRDNSWTYETAINPAV
                     ETDVWSGNADTQTLDLTPTSARLEPQVCPFCEGGYSSVGKLAAATQPLNQIFLDGEGH
                     ILITDDNGNRLGYVDGKIVNEIPGASYTKYRMLANGETPEPIYAVPADLDITVTIDGS
                     ALTEETLTDLVLIGPGYSIGVEGIYLTPGQVDVAYFYPTDEMIAYETTSDESPSIIFG
                     VENPDADYYFEVYSADMIGGGTITASLDSKAGDLLINTEQLTGEGSFNFYLTRITDEA
                     EEEFSAEDITLQEGALIYVNYAEWTDANPEGIYFGVDLNGDGEIDDEYVVDDAN"
     gene            complement(3959..4765)
                     /locus_tag="PKI33_02415"
     CDS             complement(3959..4765)
                     /locus_tag="PKI33_02415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM35881.1"
                     /translation="MLNKILFHRFPNIKSLLTRLQYEFLSTLKLNKDVMFMNFGYTAH
                     HQGQEPIPLDPDDEKHRYPLQLYHHVARHADWTNADALEVSSGRGGGASFIMRHFKPR
                     SYVGVDFSAKAIEFCREHHKVDGLRFEHGNAEDLQFPDNSFDIVVNVEASLYYPNIKK
                     FFQHVKRVLKPGGYFLYTDLRYEEKVEEWHAQIKEMGFKVIKEEDITENVLKAMEMDR
                     ERRIHLVKTYIPRILQKQFFHFAGLDVESPTSAQPHLEQRRYWYFALQKM"
     gene            complement(4885..>5106)
                     /locus_tag="PKI33_02420"
     CDS             complement(4885..>5106)
                     /locus_tag="PKI33_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35882.1"
                     /translation="PKPNRVQMKAYKVKYPRSEPDALFDLWLRDYEQASRSFSVCRFV
                     EQIGPSFGTSEALRLHDEITGANLHLPLA"
BASE COUNT         1381 a         1211 c         1158 g         1356 t
ORIGIN      
        1 ccagagttta gatgaactca aaaagtatgt tgcccaacca agtatcagcg ctcaaaactt
       61 gggactgaag gaatgtgccc aacttgtaaa agaaatgctg gaaaagcggg ggttcacagc
      121 tgagatcatg gcaacagagg gagcgcctgt tgtctttgca gaacgcaaag gcaacgtgga
      181 taaaaccatc cttatctaca atcattacga cgttcagccg ccagagccat tggaattatg
      241 ggatagcccg ccttttgagc cagagattcg ggatggcaag atgtatgggc gcggtgtgag
      301 cgatgataaa ggtcatctca catcacgctt gcatgcgatc gatgcgatcc ttgcagaaga
      361 tggcgaatta ccttgcaata tcaagttcat cattgaaggt gaagaagaaa ctgccagtgt
      421 gcacttgcac gagtttatct ccaataatgt agaacgactc aaagccgatg cctgcatttg
      481 ggaatttggc agtgttgatc atcgcgaccg cccacagcag tatttggggc tgcgtggaat
      541 ttgctatgtg gaattatcgg tcacctcgct gggcacagac gttcactctg gcattggtgg
      601 cagcatcttc cccaacgccg catggagatt gacctgggca ctgagcacgc tcaaaggcgt
      661 agatgaaaga attcgcattc ccggttttta tgatgatgtt gtgcaaccta cagaccgaga
      721 tcgtgagttc atggaaaaac tccctgacct ggcagaagat tacaaaaccc gctttggggt
      781 gcagaaattc atcaaaggct tggaaggcgg catggatttg aaacttgaag aagtcttcac
      841 acccacttgc accatttgcg gtctcaccag cggataccaa ggtcccggct cgaaaaccgt
      901 tcagcctgcc tctgcctctg caaaagtgga cttccgcctt gtgccgaatc aaatgccgaa
      961 agacatcctc aaaaaactgc gcgcccattt ggatgccgaa ggcttcagcg acgtcgaaat
     1021 taaattcttg ggtggcgagc ctgcggcgcg gacagatccg gatcatcctt tcgtcaaact
     1081 ggtcagtgaa actgcagaag aagtctacga tgcgaagatg gacattgtcc ccatgatcgg
     1141 cggttctgga cctaattatc cgttcgtgca tgaacttgga ttgcccgttg tgtgcatggg
     1201 aattgggtac ccgaatacaa gggcgcacgc ccctaacgag aacttccgta ttgatcttta
     1261 tgcgcaacat gccaagcata cggcaagggt cattcgagag tttgccaagt aagtacaaga
     1321 caaaagacca cgagcaatca ttgttcgtgg tcttttgtat cggtcgccaa tgacatgcct
     1381 aaagtgacct ttttcactcc aatttagtga ctttcttttt gcaaattctg cgtataatgt
     1441 gattgtaata aatttcacaa tataacttca ccccattgga ggaaactatg ttcgtcggcg
     1501 aaagaatgtc tcaccctgta attaccgtca ctccggaaac cccggttcat gatgcacttg
     1561 ctatgttcaa gaaagaacac atccgccggg cacctgttgt caaagacgga aaacttgtcg
     1621 gcattgtttc tgaaagtgac cttttgaacg cctcaccttc tcccgtcacc agcttgagcg
     1681 tgtgggaaat gaactacctt attagtaaag tcaccgtcaa acaggttatg agcaagaagt
     1741 tgatcaccgt cgatgtcaac acccctatcg aagaagctgc ccgcattatg gcagataaaa
     1801 aattaggtgg tttgcctgtt atgcgctccg ataaactcgt aggcatcatt accgagaccg
     1861 atctcttcaa gatattcctt gaactgatgg gcgcccgcac caaagctttg cgtgtgactg
     1921 ccgaaattga cgacaaaccc ggtactcttg ccaaactcac caaggcggta tcagatgcag
     1981 gtggcaattt catctcattt ggtcaattct ctggggacga tacttccagc aagatcctca
     2041 ctttcaaggt agctggtttg aagaaagatg aagttaccag tgcattgagc aaggttgtaa
     2101 aaaaattctg ggacattcgc cagagctaaa ttaacaaaga aaatagcgca tggacttctc
     2161 ctctccatgc gctattttta tttaagtgtt caatagctta gttggcatca tctactacat
     2221 attcgtcatc gatctcgccg tctccattga ggtccacgcc aaagtagata ccttcagggt
     2281 tggcatcggt ccattcggcg tagttgacgt agatcaatgc gccttcttgc aaagtaatgt
     2341 cttcggcgct gaattcttct tcggcttcat ctgtgatgcg ggtaagatag aagttgaaag
     2401 agccttcgcc agtcaattgt tccgtattga tgagcaagtc tccagccttg ctatcaagtg
     2461 aggcggtaat ggttccgccc ccgatcatat cggcgctata cacttcgaag tagtaatcgg
     2521 catctgggtt ttctacaccg aaaataatgc tgggggattc atcggaagtg gtttcgtaag
     2581 caatcatttc atccgttggg tagaaatacg ccacatccac ctgacccggt gtgagataaa
     2641 tgccttccac tccaatagaa tagccgggtc cgatcaaaac gaggtcggtg agggtttctt
     2701 cggtcaaggc ggagccgtca atggtaacgg tgatatctaa atcagcaggg acggcataaa
     2761 taggctcggg ggtttctcca ttggcaagca tacgatattt ggtataggag gcgccaggaa
     2821 tttcattcac aatcttgccg tctacatagc caaggcgatt gccattgtca tcagtaatca
     2881 gtatgtgacc ttcgccatcc aagaagatct ggttaagagg ctgggttgct gcggcaagtt
     2941 tgcctacact agagtatccg ccttcacaga aggggcaaac ttggggttcg aggcgagctg
     3001 aagtcggagt cagatcgagg gtttgagtat ctgcatttcc agaccagacg tccgtctcca
     3061 ctgctgggtt gattgcagtt tcgtaggtcc aagaattatc acgtgagtcg atgaataact
     3121 cgcgggtctc gccagggtag ttattatcgt aaaccagaat agcatacaaa ccatcccctt
     3181 tgtcttcgac cccaaacgga gtaatggcat gaccatcgcc gttttcatta taaataccaa
     3241 tggtataggt ttcgcccttc acatccattt tttcaactag atccaaaatc tccatgggtg
     3301 tgccgctgat gatgctggac gctgttgggt ctacggcttg ggttgcccac cagtacgcaa
     3361 tttcacgttg cagtgcttta tcagtgatat ctaattcact agcggttttt ccgccaaact
     3421 cagagggaga aacctgaccg gtatacatca ccaagcttaa cacagccatg ccttcgcaat
     3481 gaccaccgga catatcggtg ttgagctcct ccatccactg tgctgctggc ggggtgagaa
     3541 tacattcatc cccattaata tctgcacaaa cttcatcacc aaacatacgg cgtaactcat
     3601 ctgccgtcaa attagtagca gggatctcat caccataatt ttcaaaacta aacccgtttt
     3661 cttctggggt aaagccaaaa tcgtaaatca catcgccagg ggcgctgggt gttatagaag
     3721 tcgcatcact acttgcttgt gtcgcttctt ctgtgacaat ttcttctgta gcaacttctt
     3781 cagtggagaa gtcttcttca tagtcttctg aaacgccaca agctgaaaga atcaaactac
     3841 tcaaaaccaa aatcgagatc aatccaaaaa acgggtactt tttcatattt cctctagtga
     3901 atttattaca tcaaacctgc gtaacaatcg tcacgcaggc tctctttctt attatttttt
     3961 acatcttttg taaagcaaaa taccaataac ggcgttgctc taaatgaggc tgtgctgaag
     4021 ttggagactc tacatccaac ccggcaaaat ggaagaactg tttttgcaaa atgcgcggaa
     4081 tataggtctt cacaaggtga atacggcgct cgcgatccat ttccattgct ttgagaacat
     4141 tctctgtaat atcttcttcc ttgatcactt tgaagcccat ctctttaatt tgtgcatgcc
     4201 attcttccac tttttcttcg taacgcaggt cggtgtacaa gaaatatcca cccggcttca
     4261 agacacgttt cacatgctgg aaaaacttct tgatattcgg gtaatacaaa gatgcctcta
     4321 cattgaccac aatatcaaaa gagttatcgg gaaattgcaa atcttctgca ttgccatgct
     4381 caaatcgaag tccatccact ttatgatgtt cacggcagaa ctcgatagct ttggcagaaa
     4441 aatccacgcc gacgtaagaa cgtggtttga aatgacgcat gataaaagac gcgcctcctc
     4501 cccgccccga gctgacctct aacgcgtcag cattggtcca atctgcatgc cgggcgacgt
     4561 gatgatacaa ctgcagcgga tagcgatgtt tctcatcatc agggtcaagc ggaatgggtt
     4621 cttgtccctg atggtgagcg gtatacccaa aattcataaa catcacgtct ttgtttaact
     4681 tcaacgtgga gagaaactcg tattgcaagc gggtcaacag acttttgata ttcgggaagc
     4741 gatggaacaa tattttatta agcattctct ctcctatata aagttgacaa aaagcatcca
     4801 aaattggatg ctttttgtat ggttatatca attataacct cgagcatttc ttgaaaacat
     4861 tacctttcaa agctgacgag gtaactacgc taacggaagg tgaagattag cgcctgttat
     4921 ttcatcatgt aaacggagcg cttcagacgt accgaaactt ggtccgattt gctccacaaa
     4981 cctgcatacg ctgaaagatc gactcgcctg ttcataatca cgcagccaaa ggtcgaaaag
     5041 ggcatctggc tcactacgcg gatatttcac cttataagcc ttcatttgaa ctcgatttgg
     5101 ttttgg
//
LOCUS       DAOREF010000076         5101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9171_length_5101_cov_3.670234, whole genome shotgun sequence.
ACCESSION   DAOREF010000076 DAOREF010000000
VERSION     DAOREF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9171_length_5101_cov_3.670234"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKI33_02425"
     CDS             <1..418
                     /locus_tag="PKI33_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35883.1"
                     /translation="LFTKEYFAKAQKALRPGGYFLLQAGPVSPPSMDMHIRLVSTLKA
                     VFKYVHSYHIHVPMLGHPWGFILAGDTPIDVRPAPEMVDAILADKTNGKFKMFDGITL
                     LGLMQSPKHIRDAIAKETTVYTIEHPPVFSTQELPK"
     gene            492..1322
                     /gene="speE"
                     /locus_tag="PKI33_02430"
     CDS             492..1322
                     /gene="speE"
                     /locus_tag="PKI33_02430"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNM35884.1"
                     /translation="MSIWFTEELHPYYRKGIRVKRVLADEQTKYQHLQVVETEFFGNA
                     MILDGIIQLTERDNMGYHEMIVHVPMLAVEKPKRVLLIGGGDGGSLQQVLRYPSVEEV
                     SICELDQRVVDLSREHFAASFGDPWADPRSKLLIRDAFGYLEENPGTFDVIISDTTDP
                     IGMAERLFSDEFYKLMVRALAPGGAAATQCEQPFFDTELIKTIYQTAKSLTKNPAYYY
                     TNVPTYPGGGIGFMYVSNTPWENGLKTPYPPGVNQYINPEIHKAAFALPEFFRKELYG
                     "
     gene            1422..2384
                     /locus_tag="PKI33_02435"
     CDS             1422..2384
                     /locus_tag="PKI33_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HNM35885.1"
                     /translation="MNPIKGLHHVTAVARDPQRNVDFYRNVLGQRLVKRTVNFDSPDT
                     YHFYFADETGTPGSVLTFFAWPNMRRGVRGNGETNTVAYNVPVGSLSFWEKHLKQNGI
                     VPGNIQSRFNQNVLPFTDPDGMNLELVEVENLPVIQHWEDGPIPREYALHGFHSVTLW
                     VDEVEPTASLLTEGMGYRFAGAEEDRQRFKGDTNALGHIVDVVERPQKMPAGFGAGSI
                     HHIAFRVPTDDEQLKYQSVLGEAGFRVSPVMDRSYFHSIYFREQGGVLFEIATDTPGF
                     MVDEPKDLLGIKLKLPAWVEPNRDEIEASLLPLTLNPIEKASLS"
     gene            2381..3016
                     /locus_tag="PKI33_02440"
     CDS             2381..3016
                     /locus_tag="PKI33_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNM35886.1"
                     /translation="MTIPHETSPVYAAGAKLEEAKSAMILLHGRGASAQDILSLSTYL
                     NFPGMAYLAPQAEGSIWYPNRLIAPVESNEPYLSAAIAKIDGLVKHVEAGGIPVEKIF
                     IGGFSQGASLSSEFVIRNPRPYGGLLIFSGGYVWQSGKPRTAEGNLKGVPVFNGCSNV
                     DPHIPLERVKETTALLTSMGAEVNEQIYPNMDHTIMDEEIEQARALVEKRL"
     gene            3130..>5101
                     /locus_tag="PKI33_02445"
     CDS             3130..>5101
                     /locus_tag="PKI33_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNM35887.1"
                     /translation="MNTHGSVSKFIVLGVTLSLLLSLLVPSGWVSARPEQALGTISFV
                     PNIETAGVVVSGTGLPATAQLSYRRAGDVNWQPGHALLRIKDGRLIGSLFNLAASTSY
                     EVRVSDGVTEVSGSFTTQADELLFAPTNIIYVNASAQASGDGSASAPFRTIQEGVNRA
                     APGTQVLVADGIYSESVTFPASGTAGSWIQVKAQGGGAILDGSASVLATDWNPVEGKA
                     NVWFTKISPGIKYLARDGLRFYQYDFLSDLYDQRGHNNVPMSEGWFYDPINGRLYVRI
                     NREPHKYTWQVPKFNAAFMVDGRDWIWIEGFEMRYYGTEFGCGVCSKNSSHVVIRKNK
                     IHHIQNPVFVEWTGGEDRGNDTRIEYNEMYDTLTGGDWAWGAIKSTAMESMAIVLRGH
                     TGAIVRGNTIHHYFNGIYTSSSAALDNPGVAFDADIYNNYVYHIGDDAFEPEGTAVNH
                     RFRNNKVDSTLVGISLAPVTMGPVWIVRNTFTNFTARSVKWDRGSDGWVLMYHNTSFT
                     TYTNTHSVELISAVKNSILRNNIFHANGYSVEAHNPGSTGHDWNYNDWFALNSNAPFM
                     WEGISYPTLNQLCRSTGLDCNGYEDSPGLSTDMTLLSNSPNIDRGVLIPGINDQFMGN
                     APDLGAYESVYGTSLATPVATNTPLPLPTVTPT"
BASE COUNT         1265 a         1225 c         1278 g         1333 t
ORIGIN      
        1 gttgttcacc aaggaatatt ttgctaaggc gcaaaaagca cttcgccccg gcggctactt
       61 cttgttgcaa gcgggtcctg tttcgccgcc ttcaatggac atgcatatcc gcctcgtttc
      121 tacgctcaag gcggttttca agtatgtgca ttcctatcat atccatgtgc ccatgctcgg
      181 gcatccttgg ggctttatcc ttgctggtga tacgcccatc gacgtccgcc ctgcaccgga
      241 gatggtcgat gccattcttg ccgacaagac caacggcaaa ttcaaaatgt ttgacggcat
      301 cactttgctc ggtctgatgc aatcgccaaa gcatatccgc gatgcgattg caaaagagac
      361 cacagtttac accattgaac atccgcccgt attcagcacg caggaattac cgaaatagta
      421 gtaaagacct aagccgttac aagaccgccg acaacagaat ggtcggcggt ctttacttga
      481 aaggataacg aatgagcatt tggttcactg aagagttaca cccttactat cgcaaaggca
      541 tccgtgtcaa acgcgtgctt gccgatgaac agacaaaata tcaacacttg caagttgtcg
      601 aaaccgaatt ttttggcaac gccatgatcc tcgatggcat catccaactg accgagcgcg
      661 acaacatggg ctatcatgaa atgatcgtcc acgtgcccat gctggcggtg gagaagccca
      721 agcgcgtgtt gcttatcggc ggcggcgacg gtggctcgct tcaacaagtc ttgcgatatc
      781 cttccgtcga ggaagtttct atctgcgaac tggatcaacg cgtcgtggac ctctcacgcg
      841 aacacttcgc cgcctctttc ggggacccgt gggctgatcc acgctccaag ctgctcattc
      901 gggacgcctt tggctaccta gaagaaaacc ctggcacctt cgatgtcatc atctcagata
      961 ccaccgatcc tattggcatg gcagagcgtc tcttttccga tgaattttac aagctgatgg
     1021 tacgtgccct agctcctggt ggtgccgctg cgactcaatg cgagcagcct ttctttgaca
     1081 ccgagttgat caaaaccatt tatcaaaccg ccaagtcact caccaagaat cccgcctatt
     1141 actacaccaa cgtccccaca taccccggcg gtgggatagg gttcatgtac gtttcgaaca
     1201 ctccttggga aaacggtttg aaaacgccct atccacccgg tgtgaaccaa tacatcaacc
     1261 cagagatcca caaagcggcg tttgctttgc cagaattctt ccgcaaagaa ttgtacgggt
     1321 aaaataagac caagcctcca aaattaactt tggaggcttt tcatacgaaa ttcaaacgga
     1381 tatttttgcg atagagtgcg tctaggcaat aggagagaaa tatgaaccct attaaaggtt
     1441 tacatcatgt cacagccgtg gcacgtgacc cacaacgaaa tgtggatttt tatcgaaatg
     1501 ttttagggca acgcttggtc aagcgcacag ttaacttcga ctcgcccgat acctatcact
     1561 tctattttgc agatgaaacc ggcacacccg gcagtgtatt gaccttcttc gcttggccca
     1621 atatgcggcg cggcgtgcgc ggcaatggag aaaccaatac cgttgcatat aatgtcccgg
     1681 ttggttcact ttccttttgg gaaaaacacc tgaaacaaaa cggcattgtc cccggcaata
     1741 ttcaaagccg ctttaatcag aatgttcttc cttttacgga ccccgatggc atgaacctgg
     1801 aattggtgga agtggaaaat ttacccgtca ttcagcattg ggaagatgga cccatcccac
     1861 gtgaatatgc tttgcatgga tttcatagcg tcaccttgtg ggtggatgaa gttgaaccaa
     1921 ccgcttcact cttgaccgaa ggcatgggct atcgcttcgc cggagcagag gaagaccgcc
     1981 aacgctttaa gggtgatacg aatgccctcg gtcacattgt ggatgtggtt gagcgcccgc
     2041 aaaagatgcc tgcagggttt ggggctggtt ccatccatca tattgctttc cgcgtgccca
     2101 cggatgatga acaactgaaa tatcagtccg tcttgggtga ggcggggttt cgcgtcagcc
     2161 cggtaatgga tcgcagctat ttccattcca tttattttcg ggagcaaggc ggtgtgttgt
     2221 ttgagatcgc aaccgacacg cccgggttca tggtcgatga acccaaggat ttgctgggca
     2281 taaagttgaa actgcccgcc tgggtagaac ccaatcgaga tgagatcgaa gccagcttac
     2341 tgccattaac ccttaaccct attgagaaag cgagcctttc atgaccattc ctcacgaaac
     2401 atcccctgtc tatgcagcgg gtgcaaagct agaagaagcc aaatctgcga tgatcctcct
     2461 tcatggacgt ggcgcatcgg cacaagatat tctgtccctt tccacatact tgaactttcc
     2521 gggcatggcg tatctcgctc cccaagcgga aggatccatt tggtacccga accgtttaat
     2581 cgcgcctgtt gaatccaatg aaccgtacct ttcagccgcc atcgcgaaga tcgatgggtt
     2641 ggtaaaacat gttgaagcgg gcggtattcc tgtcgaaaaa atatttattg gcggtttctc
     2701 tcaaggcgcc agtctttcca gcgaatttgt gattcgcaat ccgcgcccgt atggtgggct
     2761 gttgatcttt agcggcggat atgtttggca aagcgggaaa ccccgcactg cggaaggcaa
     2821 cttgaaaggt gtaccggttt tcaacggctg ttcgaatgtg gatcctcata ttccgttgga
     2881 gcgagtgaaa gagaccacgg cactcctgac ttccatgggg gcagaagtaa acgagcaaat
     2941 ttacccaaac atggaccaca cgattatgga cgaagagatc gagcaagcaa gagccttggt
     3001 tgagaaaagg ttataagaaa tcgccccgaa aggggcgttt tttgtcatgg taccagaatt
     3061 gtatttttat aggtgcgtgc tatagtgaaa acagacaggg ttttgtttcc atccctgtaa
     3121 atggttttta tgaacacgca tggtagtgta tcgaaattta tagtgctggg tgtaaccctt
     3181 tcgctcctgt tatcactgct tgtgccgagt ggatgggtca gtgcacgccc agaacaggct
     3241 ttggggacaa ttagttttgt gcccaatatt gaaacggcgg gagttgtcgt cagcggaacg
     3301 gggctgccgg ccacggctca gttgtcttac cgccgggcag gggatgtaaa ctggcagcca
     3361 gggcatgccc ttttgcgcat caaagacgga cgtttgattg gtagcttgtt taacttggct
     3421 gcatctacct cttatgaagt gcgcgtttct gatggcgtaa cagaagtttc tggttcgttt
     3481 accactcaag ccgatgagtt actttttgcg cccacgaata ttatttatgt aaatgcatct
     3541 gcacaggcaa gtggggatgg gtctgcctct gcgccatttc gtacgataca ggaaggggtc
     3601 aatcgtgcag caccaggcac gcaagttttg gtggcagatg ggatttacag tgagtcggtc
     3661 acattcccgg cttcgggaac ggctggaagc tggattcagg tcaaggcaca gggtggaggc
     3721 gcgattttag atgggtccgc ttcggtttta gcaacggatt ggaatcctgt tgaaggcaaa
     3781 gccaatgtct ggtttacgaa gattagcccg ggaattaagt atctggcgcg ggatggattg
     3841 cgtttttatc aatatgattt tttgagtgac ctttacgacc aacgcggaca taacaatgtg
     3901 ccgatgagtg aaggttggtt ttatgaccct atcaatggtc gtttgtatgt gcgcatcaat
     3961 cgtgagccgc ataaatatac atggcaagtt ccaaagttca atgccgcgtt tatggtagat
     4021 ggacgtgatt ggatttggat cgaaggtttt gagatgcgct actatggcac agaatttggc
     4081 tgtggcgttt gttctaaaaa ttcttcgcat gttgtgattc gcaagaacaa aattcatcat
     4141 attcaaaacc cggtctttgt agaatggaca ggcggagaag accgcggcaa tgatactcgt
     4201 atcgaataca acgaaatgta cgacacactt accggtggcg attgggcatg gggcgctatt
     4261 aagagtacgg caatggagtc tatggcaatt gtcttgcggg ggcatacggg cgccattgtg
     4321 cgtggcaata ccatccacca ttatttcaat ggcatttaca ccagctcctc ggctgcatta
     4381 gacaaccctg gtgttgcctt tgatgcagat atttacaaca actatgtcta tcatatcggg
     4441 gatgatgcgt ttgagcctga agggacagcc gttaatcatc gttttcgaaa taacaaagtg
     4501 gattcgactc tagttggtat ttcgcttgcc cctgtaacca tgggtccagt ttggattgta
     4561 cggaacacct tcacgaactt tacggctcgc agtgtgaagt gggatcgcgg ctcagatggt
     4621 tgggtactga tgtatcacaa cactagtttt acgacttata ccaataccca ttcagttgag
     4681 ctgattagcg ctgtgaaaaa ctcaatcttg cggaataaca ttttccatgc aaacggttat
     4741 tctgtagagg cgcacaaccc cggttcaacg ggtcatgatt ggaattacaa cgattggttt
     4801 gcattgaatt caaatgctcc ttttatgtgg gaaggcattt catatcccac tttaaatcag
     4861 ttgtgccgtt caacagggtt agattgcaat ggctatgaag attcgccggg cttatccaca
     4921 gatatgactt tgttatcaaa tagccccaac attgatcgtg gcgttctgat tcctggaatc
     4981 aacgatcagt ttatgggcaa tgcgcctgat cttggcgcat atgagtctgt gtatggaaca
     5041 tctcttgcta cacccgttgc gaccaacaca cctttgccac tgccaactgt tactccaacc
     5101 c
//
LOCUS       DAOREF010000077         5084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9228_length_5084_cov_6.362696, whole genome shotgun sequence.
ACCESSION   DAOREF010000077 DAOREF010000000
VERSION     DAOREF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9228_length_5084_cov_6.362696"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKI33_02450"
     CDS             complement(<1..390)
                     /locus_tag="PKI33_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35888.1"
                     /translation="MIEKKNIYWFLGITVLLSWPLFLSTLFLGNMDAQTKQLTTQGLW
                     AAAMWGPGIAAIITTLFIAKVPFNSLRLNTIGPKRFYLWAWFLPTVFTVLSGLFTILF
                     GIGKLDLSFSMIRTALESTPGGSEIPAS"
     gene            complement(402..641)
                     /locus_tag="PKI33_02455"
     CDS             complement(402..641)
                     /locus_tag="PKI33_02455"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HNM35889.1"
                     /translation="MNNNELSKWEYRVQTVGNFWSGVKATELEELLNAWGEEGWEVVS
                     THVLENQNKINVIAKRPLTRETIRQRSMPNYQYEK"
     gene            complement(706..1356)
                     /locus_tag="PKI33_02460"
     CDS             complement(706..1356)
                     /locus_tag="PKI33_02460"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNM35890.1"
                     /translation="MSTKTTSILVIALVAIAVIAGVILWNQLPDQMASHWNVNDQADD
                     YMPKFWGVFLMPIISLGMLALFLVIPNIDPLKANIAEFRNIFNLFILLIVVFMLYVHG
                     LTLAWNMGNQSFKMSAAMLPFLGVLFIFIGYMLRQAKRNFFIGIRTPWTLSSDTVWNK
                     THQLGSVLFMVSGAFAFIGSFLGGMLAFWLLFVPLMGSVVFLILYSYILYRNETRA"
     gene            complement(1392..2327)
                     /locus_tag="PKI33_02465"
     CDS             complement(1392..2327)
                     /locus_tag="PKI33_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNM35891.1"
                     /translation="MNTKFSKLMNTKKKDDQHIPPIAGFVFGIILIGSIKVALILDAN
                     HASDLVTAAVMLAMIGIASYFLFAIKVASQWEKAVVLRLGKFNGLKGPGLFWLIPIID
                     TAVMWIDHRVTVTSFSAEKTLTKDTVPVDVDAVLFWVVWDAEKAALEVNDYQSAISWA
                     AQTALREIIGQMNLADILVGRAKMDEELQKIIDERTTPWGVTVQSVEIRDVVIPQVLE
                     DAMSRQAQAERERQARVILGESEKQIATSFSEASQAYIDNPTALHLRAMNMLFEGLKE
                     KGALVIVPSSAIDTMNLGGLSGMVSLAQNNAPKEK"
     gene            complement(2353..2775)
                     /locus_tag="PKI33_02470"
     CDS             complement(2353..2775)
                     /locus_tag="PKI33_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNM35892.1"
                     /translation="MTNTKLTLHLDFHSGLPIYTQIVNQIQSQLANGILKPGDQLPTV
                     RALASELRVNFNTVARAYRILDEARIISTQQGRGTFITEIPPPEVTEKLRQESLSELT
                     NRFLSEAFRLGFSEREVSQMVRDRLKSWKEAKQVKEEE"
     gene            complement(2900..4375)
                     /locus_tag="PKI33_02475"
     CDS             complement(2900..4375)
                     /locus_tag="PKI33_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNM35893.1"
                     /translation="MRIPLDHKSDIPLYQQIEDHLRKSILSGNLPADTRLPASRQLAR
                     DLGVNRTTVENAYSALEADGLVFSKMGSGTYVLPVYPIPSIPKNNSDALPLWQMNLQK
                     NVFATSDTAEDMLQAGGHPRPINFASGISDARQFPVEEFRKVIQSVMRRDQIDALEYG
                     ERRGYAPLREGIARILASQGLQTHPENVLITAGSQQAIFLASQVLLKPNDIVLVEDPT
                     YSAALDLFRTLGFQIVGIPVDSQGMQVEKLEKLLQQYHPKLIYTIPNFHNPTGTCLSS
                     ARRRELILLAGKYNVPILEDDFVGDLRYEGHTQPSLKSLDPGGQVIYISTFSKMLMPG
                     LRVGFIVADGPVYDSLLNFKRLSDLATSTLIQRALDAYMTVGRYQTYLHRSNQIFRSR
                     RDAMLDAIKKHLPSTINFELPKGGLFIWLQLPKSISANELLPIACKEGVAFSPGNSFF
                     IDGISGEDWIRLNFASQPVEDIEEGIKRLGNALKKMKPKKK"
     gene            4564..>5084
                     /locus_tag="PKI33_02480"
     CDS             4564..>5084
                     /locus_tag="PKI33_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNM35894.1"
                     /translation="MQAKTGTWTEKKGLAQMLKGGVIMDVVNAEQAKIAEDAGACAVM
                     ALERVPADIRANGGVARMSDPDMILKIMEAVSIPVMAKCRIGHFVEAQILESIGIDYI
                     DESEVLTPADEDHHILKHNFKVPFVCGCRNIGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARTVIGDIK"
BASE COUNT         1376 a         1150 c         1186 g         1372 t
ORIGIN      
        1 gcttgctgga atttcacttc cacccggcgt agactctaat gcagttcgta tcatgctgaa
       61 gctcaaatct aatttgccta ttccaaatag aatagtgaag agtccactca gcacagtgaa
      121 tacagtgggc agaaaccatg cccaaagata aaagcgtttt ggaccaatcg tattcaaacg
      181 caaactattg aatggtactt tggcaatgaa caaggtagtg atgatggctg caattcctgg
      241 accccacatt gcagcagccc ataaaccttg agtggttagt tgtttagttt gggcatccat
      301 atttccaagg aacaatgttg aaaggaataa cggccatgaa agcaacactg taattcctag
      361 aaaccaatag atgtttttct tttcgatcat tttatatctc cttacttttc gtattgataa
      421 ttcggcatcg aacgttgacg gattgtttcg cgagtcaacg gacgtttcgc gatcacattg
      481 attttgttct ggttttcaag cacatgtgtg gatacaactt cccagccttc ttcaccccat
      541 gcgttcaata gttcttccaa ctcggtggct ttcaccccag accaaaaatt cccaacggtt
      601 tgtacgcgat attcccattt ggataattca ttattgttca ttggaaccct ttcgtttcac
      661 gtgaaacaaa acgtgcgtgt ctgtaccaga cacgcacgtt ttgttttatg cacgagtctc
      721 gttacggtac agaatatacg agtataggat caagaatacg acggaaccca tcaacggaac
      781 gaataacaac cagaacgcaa gcatgccacc aagaaaactt ccaatgaagg cgaaggctcc
      841 tgaaaccatg aacaagaccg agccgagttg atgcgtttta ttccacaccg tatcacttga
      901 aagagtccat ggcgtgcgta tcccaatgaa gaagttgcgc tttgcctgtc tcagcatata
      961 gccgatgaag atgaacaaaa ccccgagaaa tggcaacatg gcggcactca tcttgaaact
     1021 ttgattgccc atgttccatg ccagtgtgag cccatgtacg taaagcatga agacaacaat
     1081 gagcaaaatg aacaggttga agatgttacg gaattccgcg atgtttgcct tgagcgggtc
     1141 aatattgggt atgacgagaa acagcgccag cattcccagt gagatgatcg gcatgaggaa
     1201 aacgccccag aactttggca tgtaatcatc tgcctgatcg ttgacattcc agtgagaagc
     1261 catttgatct gggagttgat tccataagat aactcccgcg attacggcaa tcgcgaccaa
     1321 agctataaca agaatagaag ttgttttcgt tgacattctt ttcctttctt cattcgtttc
     1381 acgtgaaacg tttacttttc ttttggcgca ttgttttgcg ccaatgaaac catgccgctt
     1441 agcccgccga gattcatcgt atcaatcgca gaagaaggta cgatgaccag agcgcctttc
     1501 tctttcaacc cttcaaagag catattcatc gcgcggagat gcaaggcagt tgggttatca
     1561 atataagctt gcgaagcttc agagaaagat gtggcgatct gcttttcaga ttcaccaagg
     1621 atgacacgtg cctgacgttc gcgctctgcc tgggcttggc gagacatggc atcttccaag
     1681 acttgaggaa taaccacatc gcgaatctct acagattgca cggtcacgcc ccaaggtgtt
     1741 gtacgctcat caatgatctt ctgaagttct tcgtccattt ttgcgcgccc aacgagaata
     1801 tctgctaaat tcatttgacc aatgatctca cgcaaagcgg tttgcgcagc ccatgaaatt
     1861 gcgctctgat aatcgttcac ttcgagcgcg gctttttctg catcccacac aacccagaac
     1921 aatacagcat ccacatccac aggtacagtg tctttggtca gagttttttc tgcagagaaa
     1981 gatgtgaccg taacacgatg gtcgatccac atcacagcag tatcaatgat cggaatgagc
     2041 caaaacaaac cgggtccctt aagcccattg aatttaccga ggcgcagaac aacagccttt
     2101 tcccattgag atgccacttt gatcgcaaac aaaaaataag aggcaatccc gatcatggca
     2161 agcatgaccg cggctgtaac aagatccgat gcatgattag catctaaaat taacgctact
     2221 ttgattgagc caattaagat gatcccaaac acaaacccag caatgggcgg aatatgttgg
     2281 tcatcttttt tctttgtatt catcaactta ctaaactttg tattcatatg tgcattctcc
     2341 taacttgaaa cgttattctt cctctttgac ttgcttcgct tctttccacg acttcaaacg
     2401 gtctctcacc atttggctta cttcacgttc agagaaaccc aaccgaaatg cttcactgag
     2461 aaatcgattc gtaagttcag agagtgactc ttgccgtaac ttctctgtca cttctggtgg
     2521 cggaatctca gtgatgaacg tgccgcgtcc ttgttgtgtg gatatgattc gtgcttcatc
     2581 cagtatgcgg taagcacgtg ctactgtatt gaagttgact ctcaattcag atgccaatgc
     2641 gcgcacggtt ggtaattgat ccccaggttt caaaataccg tttgccagtt ggctttgtat
     2701 ttgattcacg atctgggtat agattggtaa gccggaatga aaatcgagat gtaaggtgag
     2761 ttttgtgttt gtcatagagg actctgaatt gtactaattg tatcaatgat tgaattgtag
     2821 cacttaagga ctttaagtca agccccttaa gaataaaaaa ggactttgag gaaattctca
     2881 aagtcctttt cgcacagatt tattttttct ttggcttcat ctttttcaag gcgtttccca
     2941 atcgcttaat cccctcttct atatcttcta caggttgcga tgcaaagttg aggcggatcc
     3001 aatcctctcc actgatgccg tcaataaaga atgaattgcc gggcgaaaat gcgacacctt
     3061 ccttgcaagc aattggaagc aattcattcg ccgaaatgga cttgggcaat tgcagccaaa
     3121 taaataaacc gcctttcggt agctcaaaat taatagtgct tggcaaatgt tttttgatgg
     3181 catccaacat cgcatctcgc cgtgaacgga agatttgatt ggagcgatgc aaataggttt
     3241 gataacgccc caccgtcata taggcatcga gcgcacgttg aatcagcgtg gaagttgcca
     3301 agtcgctgag acgtttgaaa ttgagcaaac tatcgtagac aggtccatct gcgacgatga
     3361 aaccgacgcg cagaccgggc atcagcattt tcgaaaaggt gctgatgtag atgacttgcc
     3421 cacctgggtc taatgacttg agcgaaggtt gtgtgtgacc ttcatagcgc aagtcaccta
     3481 cgaaatcatc ttcaagaatt ggaacgttgt atttgcctgc caatagaatc aactcgcggc
     3541 ggcgggcact gctgagacaa gtgccagttg ggttgtggaa attcggaatc gtgtaaatca
     3601 gtttgggatg atattgctgt agaagttttt ctagtttctc tacttgcatg ccttggctat
     3661 cgacaggaat accgactatc tgaaaaccta atgtgcggaa gagatccaat gcggcagagt
     3721 atgtggggtc ttcgacgaga acaatatcat tgggcttcaa caagacttgt gatgcgagaa
     3781 agattgcttg ttgtgagcca gcagtgatga gaacattttc gggatgagtc tgcaagccct
     3841 ggctggcgag gatgcgggca attccttcac gcaagggtgc ataaccgcgg cgttcaccat
     3901 attcaagcgc gtcaatctgg tcacgtcgca tcacagattg aatgaccttg cgaaattctt
     3961 ccaccgggaa ttgacgtgcg tcactgatgc cgctggcaaa gttgatggga cgtggatgcc
     4021 cccctgcttg tagcatatct tcggcagtgt ctgacgtggc gaaaacattt ttctgcaaat
     4081 tcatttgcca gagtggcaac gcatctgaat tgtttttcgg aatggatggg atcggataga
     4141 ccggtaacac ataggtaccg ctgcccattt ttgaaaatac caaaccatca gcttccaaag
     4201 cggagtaggc gttttctacg gtggtgcgat tgacccccag atcacgtgca agttgacgac
     4261 tggcaggtaa acgtgtatcg gctggcaagt tgccggaaag aatacttttg cgaagatgat
     4321 cttcgatctg ttgataaagg ggaatatcac ttttgtggtc gagcgggatg cgcattttgt
     4381 ctctccaaac cgcattggta cggtgaatat atcaccaact tgtctgctga ataaggacaa
     4441 ttcgctttct tagcgtttta ttgggcaaaa gtggctggta atttattgat gtttattggc
     4501 tcttaacatc agccaatttc gtcctagaat ccaatcaaaa catattctat gtaggagaga
     4561 actatgcaag caaaaactgg aacttggacc gagaaaaaag gattggcgca aatgctcaag
     4621 ggcggcgtca tcatggatgt ggtgaatgct gaacaggcaa agatcgctga agatgctggc
     4681 gcctgtgcgg tgatggcact cgagcgcgtg ccagcggaca ttcgcgcgaa cggcggcgtg
     4741 gctcgtatga gcgaccccga catgattctc aagatcatgg aagcagtttc cattccggtg
     4801 atggcaaagt gccgcatcgg tcatttcgtg gaagcgcaga ttcttgagtc gattggcatt
     4861 gactacatcg acgaatctga agtgctgact cctgccgatg aagatcatca catcttgaag
     4921 cacaatttca aagtaccatt cgtttgtgga tgccgtaaca tcggtgaagc gcttcgtcgt
     4981 attggcgaag gtgccgcgat gattcgcacc aaaggtgaag ccggaaccgg tgacgtggta
     5041 gaagccgtgc gccatgctcg tactgtgatc ggtgacatca agaa
//
LOCUS       DAOREF010000078         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9337_length_5043_cov_4.526263, whole genome shotgun sequence.
ACCESSION   DAOREF010000078 DAOREF010000000
VERSION     DAOREF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9337_length_5043_cov_4.526263"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3593
                     /locus_tag="PKI33_02485"
     CDS             <1..3593
                     /locus_tag="PKI33_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNM35895.1"
                     /translation="EQVEAKPNLIQAVDVVISEIRFHGSAGANDDFIELYNPTTIAVN
                     LNGWRLRTSNASGTTNPIYSFNSDVFLLPGQYFLLANDTYNDVTVPDVRYGTTSITDD
                     GGVALIRGDNSFADQVGLSVGSAYKEVTPLTALATNVDRGYERKLGGLSDSCLDDGIN
                     STDFQLINPSNPQNSSFVQRLCGISTDLQLTQIVSNPLPTVGSTVDFILTLSNNGTSN
                     ATNVAVKDILPIGLTYQSYSATAGTTYDDGTGLWSIANLNVGNSVVLTITALVSTSGP
                     KTNIAEVWASDQFDLDSFAGNGSNLEDDYASMLLNLPKISGLVITNTVNNSNPLVGTN
                     VIFTIVVSNPTGNPYTATNVNVSALLPAGLTYVSHSATQGTYTSGSGSGIWTIGNVPV
                     NSSVTLTVTAKVITSTPSTYTSTVTSNEYLNNSASSSLNNPLSGEADLSINQSLDIAT
                     GVAGQVNLVISLKNDGPDTATGVSVRDLLPSGLTYVSHTATSGTYDANTGIWSVGNLA
                     NGTTITLTIKVKVAASGSSTNNTAQIWSSNQFDADTVDNSKSLEVAIADISILQSVDL
                     TATTAIFTITVTNSGPDDATLQVKSNLPDVTSTYAFVSAGASQGTYTSATGIWNLGLL
                     ADGASATLVITTNTTGTLQSHIVEVSSSDVVDPDSVPNNKVRAEDDISGLPYTDLNLT
                     QTVNTANPNIGGNVILNIKVNNAGPTTVTGVKVRDLLPSGLNYVSSTTASGSYASGTG
                     LWTIPSIASGATIELSITVNVPASGSYVSQAEIYESGLFDPDSIPANGVNGEDDISSV
                     TVSTNSIVRSVIINEVAWAGTTSSLSGDEWIELYNTTNASINITGWTLKAADGAPSIT
                     LNGTIPAGGYFLLEKDDDNTVSDILADQVYTGELSNSGETLTLSDAANKVIDTANGNG
                     GGWPAGSSSTYGTMERTSTATDSDSIWQTNTGVVKNGKNANGGDILGTPKKANSPLPT
                     PTPTPDKTATPTPLPPTATIPPRPIINEILARPGFDWNQDGSTDVFDEFIEIKNLTSV
                     EISLNGWKLSTVDGDSFNLPDVNLKPGDRVVFYGKQTNILLSDGGETVRLSNSSGKIY
                     DAFTYTVARTEDRSFCRLPDGNPGNSWFEDCIPTPNLTNTREGKSPTSPDGTDASACN
                     LPDTVPLDFLIPECRGYGNNIWDPYYWDQISGVFKKWISSETSKWDSYIE"
     assembly_gap    3612..3711
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3712..4173
                     /gene="pyrF"
                     /locus_tag="PKI33_02490"
     CDS             <3712..4173
                     /gene="pyrF"
                     /locus_tag="PKI33_02490"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNM35896.1"
                     /translation="ADCITLNPYLGKDSIDPFIGYKQKGVFLLCKTSNKGSGDLQDVT
                     LSNGETMYIHVAKLAEAWNEKNNIGLVVGSTHIDAMKSIREAAPNLWFLAPGVGTQGG
                     ELDAALNAGLRADGKGLLLPMSRSISRAENPGKAAAEVRDEILYWKNTNQK"
     gene            4228..4836
                     /gene="pyrE"
                     /locus_tag="PKI33_02495"
     CDS             4228..4836
                     /gene="pyrE"
                     /locus_tag="PKI33_02495"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNM35897.1"
                     /translation="MSSLPSHLTALADGLLDAGCIKFGEFTLKSGLKSPIYIDLRYII
                     SFPKLLETIGAAYLPLLKELNFKRIAGLPYAAIPIATAVSLQGNYPMIYPRKEVKTYG
                     TKAEIEGEYHAGETVVVIDDLATTGGSKFEAIEKLTGVGLVVKDVVVLVDRQSGAKES
                     LEQAGYNLHAVLTITQLLDYWEENGKVSKEKIAETRTFLKGS"
     gene            4840..5043
                     /locus_tag="PKI33_02500"
     CDS             4840..5043
                     /locus_tag="PKI33_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNM35898.1"
                     /translation="MTQDSMTIPLDLSSALSADAEAKALFDLLSPSHQREYIKWVEEA
                     KKTETRAKRIQKMMDMLKQGKEN"
BASE COUNT         1453 a         1051 c         1089 g         1350 t
ORIGIN      
        1 tggagcaggt agaggcgaag ccaaatctaa ttcaagcagt agatgttgtt attagtgaaa
       61 ttaggtttca tggctccgct ggcgcaaatg atgattttat agaactttat aatcctacaa
      121 caattgcggt aaatttaaat gggtggcgtt taagaacatc aaatgcaagt ggaacaacaa
      181 atcctatata ttcatttaat tcagatgtct ttttattacc aggacagtat ttcttacttg
      241 caaatgatac atacaatgat gtaacagttc cagatgttag gtatggaact acaagcatta
      301 ctgatgatgg tggggttgcg cttattcgtg gtgataactc ctttgcagat caggtcggat
      361 taagtgttgg gtcagcttac aaagaagtaa caccgttgac tgcgttagct acaaatgttg
      421 atagaggtta tgaacgcaaa cttggtgggt tatctgatag ttgcttggat gatggaatta
      481 attcaacaga ttttcagcta ataaatccta gtaaccccca aaactcttct tttgtacaac
      541 gtttatgcgg tataagtact gatttacaac ttacacaaat agtaagtaat ccattgccaa
      601 cagttggttc tacggtagat tttattttga cccttagcaa taatggtaca agcaatgcta
      661 caaatgtagc agtaaaagat atattaccta taggtcttac ttatcaatct tatagcgcga
      721 cagctggaac aacgtatgac gatggaactg ggttgtggag catagctaac ttaaatgttg
      781 gcaatagcgt tgtgttaact atcactgcat tagtaagtac atctggtccc aaaacaaata
      841 ttgctgaagt atgggctagt gatcaatttg atttagattc ttttgcgggg aacggaagta
      901 acttagaaga tgattatgct tctatgctgc tgaatttacc gaaaatttct ggtctggtta
      961 taaccaatac agtcaataac tctaaccctc ttgttggaac gaatgtaatt tttacgattg
     1021 tggtttctaa tccaacaggc aatccctata ctgctacaaa cgtcaatgtc tctgcattat
     1081 tgccagccgg tttaacttat gtgtcccatt cagctacaca agggacatat acaagtggat
     1141 caggatcagg aatatggaca ataggaaatg tcccggttaa ttcaagtgtc acattgaccg
     1201 ttactgcaaa agtcataaca agcactccat caacctatac atctacagtt acatctaatg
     1261 aatatttgaa taactcagct agtagttcat tgaacaaccc tctttctggt gaagcagacc
     1321 tgtctatcaa tcaatccttg gatattgcta caggagtcgc agggcaggtt aacctagtaa
     1381 tctcactcaa gaatgatgga cctgatacag caacaggagt atctgtccga gatttgcttc
     1441 cttccgggct tacgtatgtt tcgcatacgg caacttcagg tacttacgat gcaaatacgg
     1501 gtatatggtc tgttggaaat ttagccaacg gaacgactat tactctaacg atcaaagtta
     1561 aagtggctgc aagtggttca tctaccaata atacggcaca aatttggtca tcgaatcagt
     1621 ttgatgcgga tactgttgat aatagcaaga gtcttgaagt cgcgattgca gatataagca
     1681 ttcttcaaag tgttgacctt acagcaacta cagccatctt taccattaca gtcactaatt
     1741 ctggaccaga tgatgctact ttgcaagtga agtccaatct tccagatgta acatccacat
     1801 atgcgtttgt ctcagctggt gcttctcaag gaacatatac gtctgctaca ggtatatgga
     1861 atttagggct tttagcagat ggcgcaagcg caactcttgt aatcacgaca aatacaacag
     1921 gaactcttca atcccatatt gttgaagtat cttcatccga tgtcgttgac cctgactcag
     1981 tgccgaataa taaggttcga gcagaagacg atatttcagg gttgccttat accgacttaa
     2041 acttgacaca aacagtgaac accgccaacc caaatattgg tggaaatgta atcctgaata
     2101 ttaaagtaaa taatgcaggt ccaacaactg tcactggtgt aaaagtgaga gacctattac
     2161 caagtggttt gaattatgtc tcttccacca ctgcgagtgg aagttatgcc tctggcaccg
     2221 gactttggac tattccttcg atagcctcgg gtgcaactat tgagcttagt attaccgtaa
     2281 atgttccagc cagtggatct tatgtcagcc aggcagagat ttatgagtca ggtttgtttg
     2341 accctgactc aatccctgca aacggtgtaa atggtgaaga tgatatatcc agtgtaaccg
     2401 tctctacgaa ttccattgtc cgttcagtca tcattaatga agtcgcttgg gctggtacaa
     2461 cctccagttt gagtggcgac gaatggatag agctctataa cacaaccaat gccagcatca
     2521 acattactgg gtggacgtta aaagctgccg atggtgctcc ttctattaca cttaatggaa
     2581 ctattccagc aggcgggtat ttccttcttg aaaaagatga tgacaatact gtctctgata
     2641 tcttggcaga tcaagtctac acaggggaac tctctaatag cggtgaaacg cttacattga
     2701 gtgatgcggc gaataaggtg atcgatactg ccaatggcaa tggtggcggt tggcccgcag
     2761 ggagttcctc aacctatgga acgatggaac gtaccagcac ggcaaccgat agtgatagca
     2821 tttggcaaac aaatacgggc gttgtaaaga atggcaaaaa tgccaacggt ggtgatattt
     2881 taggaacgcc aaagaaagct aattcgccac tcccaactcc cacgcctacg ccagataaaa
     2941 cagccacacc taccccgttg ccgccaactg caacgatccc tccgcgccca atcatcaacg
     3001 agattcttgc ccgccccggc tttgactgga atcaagacgg ttccaccgat gtttttgatg
     3061 aatttatcga gatcaagaat ctcacctcgg ttgagatttc tcttaatggt tggaagctca
     3121 gcactgtaga tggagactca tttaacctgc cagatgtaaa cctcaaacct ggcgaccggg
     3181 ttgtgttcta cggaaaacaa accaatattc tgctcagtga tggcggtgag acggtccgcc
     3241 ttagcaattc aagtggcaag atttatgatg ccttcaccta taccgttgcc cgtaccgaag
     3301 atcgatcctt ctgccgtcta cctgatggga accccggcaa ctcctggttt gaagattgca
     3361 tcccaacgcc aaacctcacc aacacccgcg aaggaaaatc gcccacctca ccagatggca
     3421 cagacgcatc cgcttgtaat ttgcccgata ccgttccgct ggatttctta atccctgaat
     3481 gccgcggcta tggcaataat atttgggacc cctattattg ggatcaaatc agcggcgtct
     3541 tcaaaaagtg gatatcctct gaaaccagca aatgggattc atacattgag taaaatagac
     3601 cctaaaggct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcagattgc
     3721 atcactttga atccatattt gggtaaagat tcaattgacc cgtttatcgg ctataagcaa
     3781 aaaggggttt tcttattatg taaaacctcg aataaaggct cgggagattt acaagatgtg
     3841 acattatcga atggcgagac aatgtacatc cacgttgcga aattagctga ggcatggaat
     3901 gaaaaaaata acattggttt ggtagtgggg tctacccata tcgatgccat gaagtctatc
     3961 cgtgaagcgg ctccaaactt gtggtttctt gcacccggtg tggggaccca aggcggtgag
     4021 ttggatgcag ccttgaacgc tggcttgcga gcagatggaa agggtctctt attgcccatg
     4081 tcgcgttcta tttctcgtgc agagaatccg gggaaggcag cggctgaggt acgagatgag
     4141 attctctatt ggaagaatac aaatcaaaag taataaaacg actactgttg aagatgacct
     4201 tcaaaaaact caaataaagg aataaccatg tcttctttgc cttctcacct tactgctctt
     4261 gcggacggac ttctcgatgc tggctgtatc aaattcggcg aatttacgct caagtctggg
     4321 cttaagtcgc cgatctatat tgacctacgg tacattattt cgtttccaaa gcttttagag
     4381 accatagggg ctgcctattt gcctttgtta aaagagttga actttaaacg cattgctggg
     4441 ttgccctatg ccgcgattcc aattgcgaca gccgtttctt tacaaggcaa ctatcccatg
     4501 atctacccac gcaaagaagt gaagacctat ggcaccaaag ctgagattga aggtgagtat
     4561 catgctggcg agaccgtggt agttattgac gatcttgcta ccaccggtgg aagcaaattt
     4621 gaagccatcg aaaaattgac tggcgttggc ttggttgtaa aagatgtcgt tgtactcgtg
     4681 gaccgtcagt ctggggcaaa agaatcactg gagcaggctg gctacaacct gcatgctgtg
     4741 ctgaccatca ctcaactatt ggattattgg gaagaaaacg gcaaggttag taaagaaaag
     4801 atagcagaga cgcgaacctt tttgaaaggg agttgacgta tgacgcaaga ctctatgacc
     4861 attccactcg acctttcttc agcgctttca gcagatgccg aagcaaaagc cctgtttgat
     4921 ctactttccc cttctcatca acgggaatat atcaaatggg tggaagaagc aaagaaaaca
     4981 gaaacccgtg ccaagcgaat tcaaaagatg atggatatgt tgaaacaggg caaagaaaat
     5041 taa
//
LOCUS       DAOREF010000079         5035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9357_length_5035_cov_4.532731, whole genome shotgun sequence.
ACCESSION   DAOREF010000079 DAOREF010000000
VERSION     DAOREF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9357_length_5035_cov_4.532731"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKI33_02505"
     CDS             complement(<1..181)
                     /locus_tag="PKI33_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNM35899.1"
                     /translation="MFHERQINLKTPAELKIMREAGRINASVHAKLRELLQPGVTTAD
                     LNAAAEEVLKSHGCVS"
     gene            complement(233..883)
                     /locus_tag="PKI33_02510"
     CDS             complement(233..883)
                     /locus_tag="PKI33_02510"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNM35900.1"
                     /translation="MPTYIVLLGPPGVGKGTQARILSEETGLMHVSSGDLFRENLKNQ
                     TELGKLAKSFMDKGELVPDDVTINMIKDRISRPDCKAGAVLDGFPRTPAQADALEVML
                     KSFEGAVNAVPYITAAEHILVERASGRWTCRANGHIYHEKFNPPKQARVCDIDGSEVY
                     QRDDDKVETVTKRIRVYLEQTMPLVEYYRKAGKLIEIDGTQAVEEVTKVLLSALKK"
     gene            complement(896..2275)
                     /gene="secY"
                     /locus_tag="PKI33_02515"
     CDS             complement(896..2275)
                     /gene="secY"
                     /locus_tag="PKI33_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNM35901.1"
                     /translation="MKTTFSGWHYLWKSEDIRRKLIVTFVILVIYRLAANIPAPGVDH
                     DILAAFRSSTSGSGGFLGFLDLLSGGTVSNFSLLSMGVYPYITAQIIIQLLIPIIPSL
                     QQRIQDDPREGRRWQEKWTYYLAVPMAALSAVGQINIFNSLSIQSIGQAILPFGLFNP
                     DTALASWSVLIAMTAGTMFAIWLGELISEYGIRGQGLSLVIFAGIVARIPANFASLLA
                     DETNRWIMLTLTVIIIVLTVLAIVYVQQGRRNVPVMYPGRRVGNRMSMPVKGTMPLMV
                     NLSGMIPLIFASAILQFPAIIASYFTQSENQAVANFFVGIQNFFGATGTSNWGYWTIY
                     FFMVVVFTFFYTTVLFDQQNYGENLKKVGASVPGVLTGAPTQKYLSTVQSRITFVGAL
                     FLGIVAIMPFLLGLLLAAFNISASNSTGIFLITSSGLLIVVGVVRDTFQNIDAELKLH
                     GYQDSLLVR"
     gene            complement(2292..2747)
                     /gene="rplO"
                     /locus_tag="PKI33_02520"
     CDS             complement(2292..2747)
                     /gene="rplO"
                     /locus_tag="PKI33_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNM35902.1"
                     /translation="MKLHDLVPNPGSKKNRKRVGRGISAGQGKTAGRGTKGAGARSGE
                     GGHLYRQGGNLPFYRRLPFMRGEGFTPPNQVEFNEVNLDQLSEAFKANAEVNPESLAE
                     AHLLRDTRNPVVILGRGEMSVALKVRVHRVSAGAKAKIEKAGGSVELIA"
     gene            complement(2755..2949)
                     /gene="rpmD"
                     /locus_tag="PKI33_02525"
     CDS             complement(2755..2949)
                     /gene="rpmD"
                     /locus_tag="PKI33_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041604457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNM35903.1"
                     /translation="MPKNTSGKSIRVTLVRSAIGYTKDQKATVKALGLRRMHQTVEHK
                     DTPALRGMLNKIIHLIKIEE"
     gene            complement(2942..3484)
                     /gene="rpsE"
                     /locus_tag="PKI33_02530"
     CDS             complement(2942..3484)
                     /gene="rpsE"
                     /locus_tag="PKI33_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNM35904.1"
                     /translation="MADNQFDKQYESQDAFEERVIEIARVAKVVKGGRRFRFRVTVVI
                     GDKKGNIGMGVGKANAVPDAMRKASERARKDIRTVNIAGTTIPHESLGKVAGAKVFLK
                     PASAGTGVIAAGGVRAVLEVAGVRDILTKSMGSANVLNVVMATFDALDGLRSPANEAA
                     RRGKKAEELLPYWERRKQHA"
     gene            complement(3494..3859)
                     /gene="rplR"
                     /locus_tag="PKI33_02535"
     CDS             complement(3494..3859)
                     /gene="rplR"
                     /locus_tag="PKI33_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNM35905.1"
                     /translation="MANKNRALARERRHARVRKQVFGNPERPRLSVFKSLTGIYAQII
                     DDMDGNTVVSASSVDKELREKVKGMKKIEQAKAIGQAIAERAKSKGISSVVFDRGGFR
                     YTGRIKALADGAREGGLQF"
     gene            complement(3863..4411)
                     /gene="rplF"
                     /locus_tag="PKI33_02540"
     CDS             complement(3863..4411)
                     /gene="rplF"
                     /locus_tag="PKI33_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNM35906.1"
                     /translation="MSRIGRLPVDIPSGVQVELSGSKVRVKGPKGELSRVFSSLIDIK
                     MEDNQLKIARASDNPEERALHGTTRAVLANMIHGVSKGFEVILEVEGVGYRAEMEGKN
                     LALFVGYSHPVKIEPPAGIAFETDAKTRQIKIQGFDKELVGQVAANVRNVRPPEPYHG
                     KGLRYLGEKVRRKAGKAGKGAK"
     gene            complement(4422..4835)
                     /gene="rpsH"
                     /locus_tag="PKI33_02545"
     CDS             complement(4422..4835)
                     /gene="rpsH"
                     /locus_tag="PKI33_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNM35907.1"
                     /translation="MSFTDPIADMLTRIRNAVMAGHAQVALPSSKIKLEIAKILKEEG
                     FLEGFEVVDGETEAQKVLRLKIKYVGERRQRRAVISGLERVSKPGRRIYTKKSDIPWV
                     LSGIGVAILSTPKGVMTGARARQLGVGGEIICKVW"
     gene            complement(4856..>5035)
                     /locus_tag="PKI33_02550"
     CDS             complement(4856..>5035)
                     /locus_tag="PKI33_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNM35908.1"
                     /translation="AKKCMQYREQRRRHAVQVRNRCQRCGRPRGYIRRFGLCRICFRE
                     LALKGQIPGVVKASW"
BASE COUNT         1241 a         1336 c         1282 g         1176 t
ORIGIN      
        1 gcgatacaca tccgtgtgac ttcagcactt cctcagcagc cgcgttgagg tccgctgtcg
       61 tcacacctgg ttgtaaaagt tctcttagtt tcgcatgaac gctggcattg atgcgccctg
      121 cttctcgcat gattttgagt tcagcaggag tcttgaggtt gatttggcgt tcatgaaaca
      181 tttttgcaaa accttacttt atttgtacag agtaggatac tctggtcttt cgttattttt
      241 tgagagcact cagtaatact tttgttactt cttcaaccgc ttgagtgccg tcgatttcga
      301 tcaatttgcc agccttacga tagtattcaa ccaacggcat agtctgttca agatagacac
      361 ggatacgctt tgtaaccgtt tctactttat catcatcacg ttggtagact tcggaaccat
      421 cgatgtcgca gactcgagcc tgcttaggcg ggttgaactt ttcatgataa atgtgcccat
      481 ttgcgcggca ggtccaacgt ccgctggcac gttctacgag gatgtgttcg gcagctgtga
      541 tatatggaac tgcattgaca gctccttcaa agcttttgag catcacctcg agcgcatctg
      601 cttgagccgg ggtgcgggga aagccgtcga gcacagcacc agccttacag tccggacgag
      661 agatgcggtc ctttatcatg ttgatggtta catcatctgg aacaagctcg cctttgtcca
      721 tgaaggattt ggcgagcttt ccaagttccg tttggttctt aaggttctcg cgaaagaggt
      781 caccggatga aacgtgcatc agaccggtct cttcggaaag aattcgtgcc tgcgttccct
      841 taccaacacc aggaggtccg agcaagacga tataggttgg catttgtatt tacctctagc
      901 ggacgagcaa agagtcctga tagccgtgca atttcagctc ggcatcgatg ttctggaagg
      961 tatcgcgaac gacacccacc acaatgagca agcccgaaga agtaataagg aagatacccg
     1021 tgctgttgga agcagaaata ttaaaggctg ctagcaacaa cccaagcaag aaaggcataa
     1081 tagcgacaat accaaggaag agagcgccca cgaaggtgat gcggctctga accgtgctca
     1141 agtatttttg agttggcgca cccgttaaca caccggggac gctagctcca acctttttca
     1201 ggttttcgcc ataattttgt tggtcaaaca acacggttgt atagaagaaa gtaaatacca
     1261 caaccatgaa gaagtagatt gtccaataac cccaattgct ggtgccagtg gcgccaaaga
     1321 agttttgaat tccaacaaag aaattagcaa ctgcttgatt ttcgctctgt gtaaagtagc
     1381 tagcgataat cgcggggaat tgaaggatgg cactggcaaa gatcaagggg atcatgccag
     1441 aaaggttaac catcaacggc atggtgccct taaccggcat ggacatgcgg ttgccaacgc
     1501 ggcgaccagg gtacatgacg ggcacattgc ggcgtccttg ttggacgtac acaatggcga
     1561 gcacggttag cacaatgatg atcacggtca gggtcaacat gatccagcga ttggtttcat
     1621 ctgcaagcaa agaagcaaag ttcgcaggga tgcgagccac gataccggca aaaatgacga
     1681 gggaaagacc ctgtccgcga ataccgtatt cagagatcaa ctcacccagc caaatagcga
     1741 acatggttcc ggcggtcatc gcaataagca cagaccaaga agccaacgca gtatctggat
     1801 tgaacaagcc aaacggaaga atagcttgcc caatagattg gatggacagg gaattgaaga
     1861 tattgatctg accgactgca gagagcgcag ccatcggtac agcaagatag taggtccact
     1921 tttcctgcca gcggcgacct tcacgaggat catcctgaat tctctgctgt aaggaaggga
     1981 tgatcgggat caaaagttga ataatgatct gggcagtaat gtatggataa acacccatcg
     2041 acaacaacga gaagttggag accgtaccgc cagagagcaa gtccagaaag ccaaggaagc
     2101 cgcccgaacc agatgtagaa ctgcggaatg cagcaagaat gtcatggtcc acaccgggag
     2161 ccgggatatt cgcggcaagg cgataaatca ccaagatcac aaaggtgaca atcaacttgc
     2221 ggcgaatatc ttccgatttc cagaggtagt gccagcctga aaaggtggtc ttcataggaa
     2281 ggttcctcga attaagcgat caattcaacc gagccgccag ccttttcgat cttggctttg
     2341 gcaccggcgc tcacacgatg gacgcgcact ttcagggcaa cgctcatctc accgcgtccc
     2401 aaaataacca cggggttgcg ggtgtcgcgc agaagatgcg cttcagcgag ggactcagga
     2461 ttcacttctg cattggcttt gaaagcctca gaaagctggt ccaagttcac ttcattaaat
     2521 tcaacctgat tgggcggagt gaagccttca ccgcgcatga acggcaagcg gcgatagaaa
     2581 ggcaggttac cgccttggcg atacaagtga ccgccttcac cagagcgcgc acctgcaccc
     2641 ttggtaccgc gaccggcagt tttaccctga ccggcagaaa taccgcgtcc aacgcgtttt
     2701 cggttctttt tagacccagg attgggtaca agatcgtgta gtttcatgtt caacctactc
     2761 ttcaatcttg atcaagtgaa tgatcttatt caacatgcca cgcaaagcgg gcgtgtcttt
     2821 gtgttcaaca gtttggtgca ttcggcgaag accaagcgcc ttaacggtcg ccttctggtc
     2881 tttggtgtaa ccaatggcgc tgcgcaccaa ggttacgcga atgctcttac cagaagtatt
     2941 cttaggcatg ctgcttcctc cgttcccaat aagggagcaa ttcttcagcc ttcttgccgc
     3001 gacgggcagc ttcgttggca ggagagcgaa gcccatccaa cgcatcgaag gttgccatca
     3061 ccacattgag gacgttcgcg cttcccatgg atttggtgag gatgtcgcgc acaccagcca
     3121 cttccaacac ggcacgcaca ccacctgcgg cgataacgcc ggtaccagcg gatgcaggct
     3181 taaggaagac tttggcgcca gctactttac cgagcgactc atgtgggatg gtcgttccgg
     3241 caatgttgac ggtgcgaatg tccttgcgag cgcgttcaga agccttgcgc attgcatcgg
     3301 gcacagcatt ggctttacca acacccatac cgatattgcc cttcttgtca ccaatcacaa
     3361 cggtcacgcg gaagcggaag cggcgaccac ctttcaccac tttggcaacg cgggcgattt
     3421 cgatcacgcg ttcttcgaag gcatcctgcg attcgtactg tttgtcaaat tgattatcag
     3481 ccatttcctt ctcctagaac tgcaagccgc cttcgcgcgc gccatctgcc agggctttga
     3541 tacgcccggt gtagcggaaa ccaccacggt caaatacgac cgaagaaatt cccttggact
     3601 tcgcgcgttc agcaatcgcc tgaccgatgg ccttagcctg ttcgatcttc ttcatgcctt
     3661 tcactttttc gcgcagttct ttatcaacgc tggaggcaga aacaacagtg tttccatcca
     3721 tgtcgtcgat gatctgggca tagatacccg taaggctctt gaaaacgctc aagcgcgggc
     3781 gttcgggatt tccaaacact tgcttgcgaa cacgcgcatg acggcgttca cgagccagag
     3841 cacgattttt gttagccatg gattacttgg ctcctttccc ggctttacca gctttgcggc
     3901 gaaccttttc gccgagatag cgtaagcctt tgccatgata gggctctggc gggcgcacgt
     3961 tacgaacgtt cgcagccacc tgccccacta attctttatc gaagccctga attttgatct
     4021 gacgggtctt cgcgtctgtt tcaaaagcga tgccagcagg cggctcaatc ttgaccgggt
     4081 gggagtagcc cacaaacaaa gccagattct tgccttccat ttcggcgcgg taacccacgc
     4141 cttccacttc gaggatcact tcaaatccct tgctcacgcc atggatcatg ttggcgagaa
     4201 cggcgcgggt ggtgccgtgt agagcacgct cttcagggtt atccgaagcg cgggcgatct
     4261 tcaactggtt atcttccatc ttgatatcga tcaagctgga gaacacgcgt gaaagctcac
     4321 ctttcggtcc cttcacacgg accttggagc cggataattc cacctgcaca ccgctgggga
     4381 tgtctacggg taaacgacca atgcgagaca caataaacct cctaccaaac cttgcagatg
     4441 atctcgccgc ccacgcccaa ctgtcgggca cgtgcgccgg tcattacacc cttgggggtc
     4501 gaaaggatgg caacgccaat tccagagaga acccagggga tgtctgattt cttggtgtag
     4561 atacgacgac cgggcttgct gacgcgctcc aaaccggaga taactgcacg tcgctggcgg
     4621 cgttcgccta catacttaat cttgaggcgc aaaactttct gcgcttcggt ttcgccatcg
     4681 accacctcga aaccttcaag gaaaccttct tccttgagga tcttggcgat ttcaagcttg
     4741 atctttgagc tcggcaaagc aacctgggca tggcccgcca taacggcgtt gcgaatgcga
     4801 gtcaacatat cggcaattgg atcagtgaat gacatgatta acctccaacc cggttttacc
     4861 aggacgcctt tacgacgccg gggatctggc ccttcaacgc caattcacgg aaacaaatgc
     4921 ggcacaagcc gaagcggcgg atgtatccgc ggggtcgtcc gcagcgctgg cagcgatttc
     4981 tcacctgcac agcatggcgg cggcgctgtt cacgatattg catacatttc tttgc
//
LOCUS       DAOREF010000080         5025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9387_length_5025_cov_4.718511, whole genome shotgun sequence.
ACCESSION   DAOREF010000080 DAOREF010000000
VERSION     DAOREF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9387_length_5025_cov_4.718511"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKI33_02555"
     CDS             complement(<1..592)
                     /locus_tag="PKI33_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNM35909.1"
                     /translation="MSLPFIYILQSHSSHVIPDLQKRYENRYRVQGAGSGADALSSLQ
                     KHRENGENVALLLVDQSLPDMPGTDFLAKAANFFPEARKVLFAQAKDTESAITAINQL
                     GIHYYLVQPWQTAYAAKLDTLLEDWRAAIQLPYMYVKGIMDTHMARIREDSNLHVAAE
                     IVALSGVSDLMVVNDNGSFVGVLSEGDILRAALPDID"
     gene            complement(662..1039)
                     /locus_tag="PKI33_02560"
     CDS             complement(662..1039)
                     /locus_tag="PKI33_02560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNM35910.1"
                     /translation="MTDKKAVFPEGATILGAYSPGLISGGFLFISGQVGKAPDGTLGA
                     TIEEQAKQTIENIGAILRAAGCDYKDVVKATVFITKSEYFQPFNKIYSEYFPEPRPAR
                     ATVLSQLVDPALLIEIEAIAKLP"
     gene            complement(1173..1427)
                     /locus_tag="PKI33_02565"
     CDS             complement(1173..1427)
                     /locus_tag="PKI33_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35911.1"
                     /translation="MEVSANMGLGGVIAFVAGIWVLIKLFQKEGLMKGILGFICMLYT
                     FIWGLQNMKNEDLKLKTPMYLWIGGIVLGIIINFVAGASG"
     gene            complement(1454..1873)
                     /locus_tag="PKI33_02570"
     CDS             complement(1454..1873)
                     /locus_tag="PKI33_02570"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNM35912.1"
                     /translation="MTNSLSAIWHQPTFANLLESRTETGAIVAFGVVHVGLNLFGVSL
                     WQCPILAATGVPCPGCGLTRATMQLLHGDLIASLQTHAFAPIFMIALILMGLVFILPT
                     NLSQNIISSVRRLEVRNGLTAWVLCSLMLYWAVRLMA"
     gene            complement(1964..2998)
                     /locus_tag="PKI33_02575"
     CDS             complement(1964..2998)
                     /locus_tag="PKI33_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM35913.1"
                     /translation="MIYPFLAIVKKELSSVLRDRTIVISILIQFFIASFSSGLLLGML
                     SLYDADTIMKFSGPNMRIGVIESADTALADRIEERGLNVIRFETLAEAQTAFYNNEIL
                     AIVDTPTDVNGLTEVKLYLPDSDTVSSLIRMVIQEPLKQYENDLRQANGVEIYYTDLK
                     GKPATSFEFVYSVLIPMLMFFPAFVAGSMSIDAITEEVEGKTLQTLLSTPLTVSTMIG
                     AKIVSAVILAVLQCAAWLALIQINGTVIQNRAWILLLALVIAGITSTSAALSATFLQD
                     RERSQFIYSLLLLAGVGISTLLNISPITTLSRLAIGDAYTNGWDVLVFALAFAALFGF
                     LLRASRRFNA"
     gene            complement(2995..>3347)
                     /locus_tag="PKI33_02580"
     CDS             complement(2995..>3347)
                     /locus_tag="PKI33_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35914.1"
                     /translation="FIEEVVKTVGIVVLAERGVIKSVREILWLSFLSALGFLIGEKLL
                     VFTSVSVVAQAEVSTALFQAGVFLLIPLAAHFIFVSVVNLLRFKLRFPYWLALAVGTI
                     LHVIYDATVLRGGI"
     assembly_gap    3348..3447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3448..4778)
                     /locus_tag="PKI33_02585"
     CDS             complement(<3448..4778)
                     /locus_tag="PKI33_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM35915.1"
                     /translation="MNQILVIAWREITRLRKRFGGGGSPLTVLILLGVLGFTSYTLRD
                     SVTLGNGMYRVGVSGNVPAINDSRFAVMEVGADEGQALLAAKKIDLLIVDTQVFSRED
                     DKSQFAERAMKQYMEKEELVRVGNSFAEGEAFPLRVGINYLGAGQPSAGNEAGTSAPQ
                     TTKPEEVIIPSLTPPPAPFTQVLVALIYILPITFISIFFTSSFMDEKANRRLTVLLSA
                     PVSPFQIIFGKMLPYAIFALSTTVLIAYFTKANIPLALAIFAPTTLFIFAIYLMVPLF
                     YRTFKDTTFISMLVTTMTTAYLVFPAMFTNSSDLAYISPLALAVKMYRNEPFGWREYL
                     LPSLPMTAVFVFAMFAGTRLLNEEFLMGYKPLTRKVSDAIYLMMAHSKPWFAVMLWSF
                     LVIPLVYMAQVVMLAFATNLPVRLIVGAVLLAAGFIEEVVKTVGIVVLAERG"
BASE COUNT         1380 a         1177 c         1224 g         1144 t
ORIGIN      
        1 cgtcaatatc gggcaatgcc gcacggagaa tatccccttc ggagagcacg ccgacgaatg
       61 agccattgtc atttacgacc atcaaatcgc tgacaccgga gagggcaacg atctctgccg
      121 caacgtgcaa gttggagtct tcgcggatgc gcgccatgtg cgtgtccatg atgcctttga
      181 catacatgta tggcaattgg atcgccgcgc gccagtcttc gagcagagtg tcgagcttgg
      241 ctgcataggc tgtctgccac ggttggacga gataataatg aatgcccagc tgattgatgg
      301 cagtgattgc ggactcagtg tcctttgctt gtgcaaacag aaccttgcgc gcttcgggga
      361 agaagttggc ggctttggcg agaaagtccg tgccgggcat atcgggcagg gattggtcca
      421 ctaaaagaag cgcaacattc tcgccgtttt cacggtgctt ttggagggat gagagtgcat
      481 ccgcaccgga gcctgcccct tgaacacggt agcgattctc gtaacgcttt tggaggtctg
      541 ggatgacgtg tgatgaatgg gattgaagga tatagatgaa tggcagggac atgatgtctc
      601 cagaaggtga ccgcagacca tagaccgcgg tctgcggtct atggtctgcg gtctgaatta
      661 tttatggaag tttggcgatg gcttcgatct cgatcagtag agcggggtcc actaactggg
      721 agagtaccgt ggcacgtgcc ggacgtggtt cggggaaata ttcggagtaa attttattga
      781 acggctggaa gtattcagat ttggtgatga agaccgtagc tttgacaacg tctttatagt
      841 cacagccagc cgcgcggagg atggcgccga tgttctcaat agtctgtttg gcttgttctt
      901 cgatggttgc gcctaaggtc ccatcgggag ctttgccgac ctgacccgag atgaataaaa
      961 atccgccgct aatgagcccg ggcgaatagg cacctaaaat ggttgcacct tcagggaata
     1021 ctgctttttt gtctgtcatg ttaatgtctc ctttaacgct aagttggctc agtatacctg
     1081 aatgggttct ctgaaaaatc ccccaatggg ttgaacttct taaaatagaa cagcctccca
     1141 aaaaggaggc tgttctattt taattgggca aattagccgc tggcaccggc aacaaagtta
     1201 atgataattc ccagaacgat accgccgatc caaagataca tgggggtctt gagtttgagg
     1261 tcttcgttct tcatgttctg aagaccccag atgaaggtat aaagcatgca gataaagccc
     1321 aagatgcctt tcatcaaacc ttctttttgg aacaacttga tcagaaccca gatgcctgcg
     1381 acgaaggcaa tcacaccacc aagccccata tttgcagaaa cttccatttc acactctcct
     1441 agatgaattg gttttatgcc attaggcgta ctgcccaata tagcattagc gaacataaca
     1501 cccatgcagt aagcccgttt cgcacttcta gccgcctaac ggaactgata atattttgcg
     1561 acaggttggt tggcaatata aatactagcc ccatcaaaat cagggcgatc atgaaaattg
     1621 gcgcaaaggc atgggtttga agggaggcaa tcaaatctcc gtggagcaat tgcatggtgg
     1681 cacgggtcaa accgcagccg gggcatggga ctccagtagc agctaggata ggacactgcc
     1741 agagtgaaac cccaaacaga ttcagcccta catgcaccac tccaaaggcg acgatggcgc
     1801 cagtctctgt gcggctttca agcaagtttg caaatgtagg ttgatgccag atggcggata
     1861 aagagttggt catgttaagg aaaaccctct tgctttttaa aagttagcag gggaattaag
     1921 agcttgggtg aagtaacagg ctagagcgga gtctgaggcg atgttaggcg ttgaagcggc
     1981 gtgatgcgcg caagagaaat ccgaaaaggg cggcaaatgc tagggcaaag accagcacat
     2041 cccaaccatt ggtgtaggca tcaccgatgg cgagccttga aagcgtggtg atgggggaga
     2101 tgttaagcaa ggtactaatg ccaacgccag ccaacaatag caaggagtaa atgaattgtg
     2161 aacgttcgcg atcttggaga aaggtagcgc tcaatgccgc agaggtggat gtgattcctg
     2221 caatgaccag cgccagtagc aaaatccagg cgcggttttg aatgacagtt ccgttgattt
     2281 gaatgagagc caaccatgcg gcacactgca agacagccag aatcaccgcc gagacaatct
     2341 tggcaccgat cattgtgctg acggtgagcg gcgtagaaag taaggtttgc agcgttttgc
     2401 cttctacttc ttccgtgatg gcatcaatag acatactacc tgctacaaac gcagggaaga
     2461 acatcagcat aggaatcaga acggaataga cgaactcaaa cgatgtcgct ggttttcctt
     2521 ttaagtcagt gtagtaaatt tcaacaccgt tcgcctgacg taagtcattt tcgtattgct
     2581 taagcggttc ttgaatcacc atacggatca acgaactgac cgtatcggag tctggtaaat
     2641 acagttttac ctcagtcaga ccatttacat cggtcggtgt atccacgatg gcaaggattt
     2701 cgttgttata aaaagcagtt tgcgcctcag ccaaggtttc aaaacgaatc acgttaagtc
     2761 cgcgttcttc gatgcggtct gcaagggcgg tatctgcgct ttcaataacg ccgattcgca
     2821 tattgggtcc gctgaatttc atgatcgtat ccgcatcgta aagggaaagc attcccaata
     2881 acaaaccgga ggaaaacgaa gcgataaaga attggatcaa gatcgaaatg acaatggtcc
     2941 ggtcgcgcag gacggaactt agttcttttt ttacgatcgc caagaatgga tagatcatat
     3001 tccacctcgt aacacggtcg catcatagat cacatgcaga atggtgccta ctgccaacgc
     3061 caaccaatac ggaaaacgca atttgaacct caataaattt acgaccgaga caaaaatgaa
     3121 atgagcggca agtggaataa gcaagaaaac cccggcttgg aacaacgctg ttgaaacttc
     3181 ggcttgtgca accacactca cggatgtaaa gaccagcagt ttttcgccga tcaaaaatcc
     3241 caatgcagag agaaacgaga gccataaaat ttcgcgcaca gacttaatca cgccgcgttc
     3301 tgccaatacc acgattccaa ccgttttaac gacttcttca atgaaacnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnccg cgttctgcca ataccacgat tccaaccgtt
     3481 ttaacgactt cttcaatgaa accagccgca agtaacacag caccaacgat caacctcacg
     3541 ggcaggttgg tggcgaatgc caacatcacc acttgcgcca tgtacaccaa agggatgacg
     3601 agaaaactcc acagcatcac cgcaaaccag ggcttggaat gtgccatcat caaataaatc
     3661 gcatcactga ccttgcgggt gagcggtttg tagcccatca aaaattcttc gttcagcagg
     3721 cgcgtccctg caaacatggc aaacacaaac acggcggtca tcggtaaaga aggcagtaaa
     3781 tattcacgcc agccaaacgg ttcattgcga tacattttta cagccaatgc cagcggcgaa
     3841 atgtatgcca gatcactgct gttggtgaac atcgccggaa aaacaagata cgccgtcgtc
     3901 atggtcgtca ccaacatgga aatgaaggtc gtatctttga aggtgcggta aaacaatggc
     3961 accatcaaat agatggcaaa aataaaaagg gtcgtcggtg caaagatcgc cagcgctagt
     4021 ggaatatttg ctttcgtaaa gtaagcgatc agaaccgttg tggacagtgc aaaaatagcg
     4081 tatggcagca tcttgccgaa aataatctga aacggagaga ccggtgcaga aagtaaaacg
     4141 gtcaaacggc ggtttgcttt ctcatccata aaactactcg tgaaaaagat gctgatgaat
     4201 gtgatgggca aaatatagat caatgccacc aacacttgtg tgaatggagc cggaggtgga
     4261 gtcaatgaag ggatgatgac ttcctctggc tttgtagtct gaggcgcgga tgttcccgct
     4321 tcattccccg ccgagggctg cccggctccg aggtagttaa tccccactcg cagcggaaac
     4381 gcctctcctt ctgcgaacga attccctaca cggacaagtt cttccttctc catgtattgc
     4441 ttcatcgccc gctctgcaaa ctgagactta tcgtcttcac gtgaaaaaac ttgtgtatcc
     4501 acgatcagca ggtcaatctt tttcgctgcc agcaaggctt gaccctcatc cgcacccact
     4561 tccatcacgg cgaagcgact gtcgttaata gcaggcacat ttccagatac gcccacacga
     4621 tacattccat tgcctaatgt gacagagtca cgcaaagtgt atgaagtgaa gcccagaacg
     4681 cccagcaaaa tcagcacggt caacgggctg ccgccacccc caaatcgttt tcttaaacgg
     4741 gtaatttcac gccatgcaat aacaaggatt tgattcatct gtttccactt ttgtaaggaa
     4801 gcaaaagttt ctgtcttctg caaacccctt accttgagtt tgtttggtat aaattggcat
     4861 catgatcaag cttacagacg tcactaagcg gtacgataat actacagtcg tccacccctt
     4921 gaacttacaa atcaatgagg gtgagatcgt cggaatcatc gggcacaatg gggctggcaa
     4981 aagcaccacc atgaaaatga tcgctggttt ggtcgaaccg acctc
//
LOCUS       DAOREF010000081         4980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9515_length_4980_cov_3.979289, whole genome shotgun sequence.
ACCESSION   DAOREF010000081 DAOREF010000000
VERSION     DAOREF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9515_length_4980_cov_3.979289"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="PKI33_02590"
     CDS             <1..2121
                     /locus_tag="PKI33_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNM35916.1"
                     /translation="VVRMTTRPPVPRRTRILQYLMIAVVALASFGMLISPLSLNPNQQ
                     TYDIGEVTQSSIRSPRDIEYVSEIRTEEARKAAESAVQSVYSPPDPSITRAQIERLRT
                     SLLNISAIRDNKELTNEEMRQSLLSLSDLRLKDETIDYLLGISSTRWQSVQDEAVRVL
                     EQVMRRAIYEDRVEAAQSSISSSVSLTFNEQQTELVTELVSPFVIANSFFSQELTDAE
                     KLRAREAIQPIVQTYKAGETIVPAGEIITPADVEAFQQLGMIRPGQRWEDIAATAAIV
                     LLCAAFVPIYFYRKPRSVFLNDPKNLTIISLLFVVFLAGARLLAERTLAPYGYPIQAA
                     GLLLTALFSMEVGLVFAIPLAVLSAFGLPNSADLIPYYMFTSLIGLLALGPARRFWGF
                     LRAGIAISISGAAVLMAFRIPLITLDWLGILQYMGMIAFAGFSSASIALLLQYIFAQI
                     LGLTTALQLLDISRPDFPLLQFFLRNAPGTYQHSLQVANLAEQAAEKISADPLLTRVG
                     ALFHDIGKALNPNFFIENQVQGNLNAHNNANPEEVSATIIRHVTDGVQLAKKHRLPRR
                     LHDFILEHHGTLITHYQYNQAMEAVGGDISKVDIEKFRYPGPRPASRETALLMLADAT
                     EARARAERPTNDEEIRALVTSVIQTVQKYNQLDDTLLTLRDLRLITDSFVTTLRGTYH
                     PRIQYPKAAVPDQDTTLLVTRKEK"
     gene            2118..2588
                     /gene="ybeY"
                     /locus_tag="PKI33_02595"
     CDS             2118..2588
                     /gene="ybeY"
                     /locus_tag="PKI33_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNM35917.1"
                     /translation="MIHIDNQMDFRDTALLERAALVTLESVTPLNPDLGPDSAHADMS
                     IVLTDDRQLHELNLDYLGVDAPTDVLSFPAGEVDPETEALYLGDLAISIPRATQQAQN
                     GGHPVEAEAQLLVVHGTLHLLGYDHGTDEEKAVMWAEQAKVLERLGLSRIKIQE"
     gene            2591..2977
                     /locus_tag="PKI33_02600"
     CDS             2591..2977
                     /locus_tag="PKI33_02600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNM35918.1"
                     /translation="MKEFILSRIRSFRYAFQGWGYVLRTQRNAWIHSAIATAVFIVGL
                     WLQLPARDWAVIILTAALVFSAEFINTAIEAVVDLATEEHHPLAKIGKDVGAAAVLVA
                     ALAAVLIGLLILGPPLLQKIKSIYAF"
     gene            3021..3878
                     /locus_tag="PKI33_02605"
     CDS             3021..3878
                     /locus_tag="PKI33_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNM35919.1"
                     /translation="MALSFKFGTVGSPSGTPKKPGGTIGAIAFSKSIGLETLELGWVQ
                     SVRVSEETCALIKTTRDEHDFALSVHAPYFINLNAEKEEWPKSRKRLMDAAHYGYLAG
                     VTDIIFHPGSYFGNDPAEVLKVAIPRLEGCVKELQKNGDKVNLRPETMGKSAMLGSFE
                     DALAMSKAMDWVQPCIDFAHLHARPGDGSMNSYDEWSRLLEKYGKTLGAKALKQLHIH
                     LSGIEYGPKGEKNHLPLSEADLDLKALFKALHEFGCGGRILCESPIMEEDALVMKKTW
                     MKVSGEKEK"
     gene            4026..4340
                     /locus_tag="PKI33_02610"
     CDS             4026..4340
                     /locus_tag="PKI33_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35920.1"
                     /translation="MTITLPILDLFFCTVLFFLFAFCFYQGWEICFMQKNEPLFPYQV
                     IALIASYLGKEDEFRERTSHLRTSFLLRAQGVSALLGGALGIVLCVVFFINIISRVII
                     NE"
     gene            complement(4429..>4980)
                     /locus_tag="PKI33_02615"
     CDS             complement(4429..>4980)
                     /locus_tag="PKI33_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNM35921.1"
                     /translation="VERWFLNDEQVGEYSYTWEWGESGLFGTFLPNDGNPMPAGTYRL
                     EMYAGDAMTPMGNSSTVTVASGGGNSGQQSQPSSSGDTISVYGQVYDAATNNPISGAY
                     VFVLTSGTTYDEWESSDYADKYIETYLQTDSNGKYKVTDISRNKEFTLVFAAQGYYDA
                     SADNMIANSSDPDSFELNVGLNK"
BASE COUNT         1194 a         1205 c         1199 g         1382 t
ORIGIN      
        1 gtcgttcgta tgaccacacg cccccccgtt ccacgccgta ctcgaatatt gcaatacctg
       61 atgatcgccg tcgttgcact ggcttcattc ggtatgctca tttcgccttt gagcttaaac
      121 cccaatcagc agacttatga tattggggaa gtgactcaat cttcgatacg gtcgccccgt
      181 gatattgagt atgtgagtga gatccgcacg gaagaagctc gcaaagccgc tgagagcgcg
      241 gttcaatcgg tatatagccc gccagacccg tccatcaccc gcgcacagat cgaacgtttg
      301 cgcacttctc ttttgaatat ctctgccatt cgcgataata aagaactgac caatgaagaa
      361 atgcgtcaga gtttgttatc actgagtgac ttgcgtctga aagacgagac gatcgattac
      421 ctgctgggga tttcaagtac acgttggcaa tctgttcaag atgaagccgt gcgcgttctg
      481 gagcaggtga tgcgccgtgc catttacgaa gaccgtgttg aagcggcaca atcgagcatc
      541 tcttcttctg taagcctgac ctttaatgaa cagcaaaccg aactggtgac agaattggtt
      601 tcgccgtttg tgatcgcaaa cagcttcttc agccaggaat tgaccgacgc tgagaagttg
      661 cgcgcgcgcg aagccattca acccattgtg caaacctata aagcaggcga aaccatcgtc
      721 ccagcagggg agatcattac accggcagat gtggaagcct ttcaacaact aggtatgatt
      781 cgtcccggtc agcgttggga ggacattgct gctacggctg ctatcgtttt gctgtgcgcc
      841 gcttttgttc ccatttattt ttatcgcaaa ccgcgttctg tattcctgaa cgacccgaag
      901 aatctcacga tcatctccct gctctttgtg gtctttttag caggggctcg tctgttagca
      961 gaacggactc ttgcgcccta tggctatcca atccaagcgg ctgggttatt gctcactgcc
     1021 ttatttagca tggaagtggg cttggtcttt gccattccgt tggcggtctt atctgcattt
     1081 ggtttgccca actctgccga tcttattccc tattacatgt tcacatcgct gattggtctg
     1141 ctggcgttgg gtccggcacg ccgtttctgg ggcttcttgc gagcaggcat tgccatttct
     1201 atttcaggtg ccgcggtgct gatggcattt cgcattccgc tcatcacatt ggattggctt
     1261 ggcattcttc aatacatggg catgatcgcc tttgcaggct tctcttctgc gagcatcgcg
     1321 cttttattgc aatacatctt tgcgcaaatc ctcgggctga ccactgcctt gcaattacta
     1381 gatatttcac gccccgactt tccgctttta caatttttct tacgcaatgc gcccggcacc
     1441 tatcaacata gtttgcaggt tgctaatttg gcagagcaag ccgccgaaaa aatcagtgca
     1501 gaccctttgc tcactcgcgt aggcgcttta tttcacgata ttggtaaggc gctcaacccg
     1561 aacttcttta ttgagaatca agtgcagggc aatctcaacg cacataataa tgctaacccc
     1621 gaagaagttt ctgccaccat tattcggcat gttactgatg gtgtgcagct tgccaagaaa
     1681 catcgccttc cgcgccgctt gcatgatttc atccttgaac atcatggcac gctcatcaca
     1741 cactatcaat acaaccaagc catggaagcc gtaggtggcg acatctctaa agtggacatt
     1801 gaaaaattcc gttatcccgg tccacgcccg gcttcgcgcg aaaccgcctt gctgatgttg
     1861 gcagatgcca ccgaagcccg tgcccgtgca gagcgcccga ccaacgacga agaaattcgc
     1921 gcactcgtta ccagcgtgat tcagaccgtc caaaaatata accagctcga cgataccctc
     1981 ctcactttgc gcgacttgcg tctcatcact gattctttcg tcaccacctt gcgcggcaca
     2041 tatcatccgc gcattcaata tcccaaagcc gccgtacccg accaggatac gaccctgtta
     2101 gttacgagaa aagaaaaatg attcatattg ataaccaaat ggatttccgc gataccgcac
     2161 tgcttgaacg tgcggcgctt gtgactcttg aatctgtcac gcctctcaac ccggaccttg
     2221 gaccggactc tgcccatgca gatatgtcca tcgtcctaac cgatgaccgc caacttcacg
     2281 agctaaatct cgattatctc ggcgtggatg cccccacaga tgtgctctcc tttcccgctg
     2341 gcgaagtgga ccctgagacc gaagcactct atcttggtga cctggctatc tctattccgc
     2401 gcgccacaca acaagcgcag aatggggggc accctgtaga agccgaggcg cagctgcttg
     2461 tggtgcatgg gacgttacac ttgcttggtt atgaccacgg cacggatgaa gaaaaagccg
     2521 tgatgtgggc agaacaagcc aaagttttag aaagactcgg tttgtctcgc atcaagatac
     2581 aggaataatc gtgaaggaat ttatcctctc gcgcatccgt tcgtttcgat atgcttttca
     2641 aggctggggc tatgttctgc gaacgcaacg caatgcatgg attcatagcg ctattgccac
     2701 ggctgttttc attgttggtt tgtggcttca actcccggca cgagattggg cggtgatcat
     2761 ccttacggca gcattggttt tttctgctga gttcatcaat accgccatcg aagccgttgt
     2821 ggaccttgct acagaagagc atcatccgct ggcgaagatt ggcaaagatg ttggcgcggc
     2881 ggctgtgtta gtggcggcgt tggcggctgt tctcattggc ttgctcattt tgggtccgcc
     2941 gttgttgcaa aaaatcaaat ccatatacgc tttctaattg acgaattact gttactcatt
     3001 tctattttct ggagactctc atggcactct ctttcaaatt tggcacagtt ggttcaccct
     3061 ctggcactcc caagaaaccc ggcgggacga taggggcgat tgcatttagc aagtcgatcg
     3121 gtttggaaac gctggaattg ggctgggtgc aatcggtgcg ggtgagcgaa gagacttgtg
     3181 cattgatcaa aaccacaaga gacgaacatg atttcgcatt gagcgtccat gcgccatatt
     3241 tcatcaactt gaatgcagag aaggaagaat ggcccaaatc tcgcaaacgt ctcatggatg
     3301 cggcgcatta tggttacctt gctggggtaa cagatattat ttttcacccc ggctcgtatt
     3361 ttggcaatga ccctgccgaa gtgttgaagg tggcaattcc gcgtttggaa ggttgtgtga
     3421 aagaattgca aaagaacggt gataaagtga atttacgccc tgaaacgatg ggcaaatctg
     3481 ccatgcttgg ttcttttgaa gatgcgcttg ccatgtcgaa agctatggac tgggttcagc
     3541 cctgcattga ctttgcccac ctccatgccc gccccggtga tggttcaatg aatagttacg
     3601 atgaatggtc tcgcttgtta gaaaaatatg gcaagacctt gggtgccaaa gccttgaagc
     3661 agttacacat tcacctctct ggcatcgagt atggaccaaa aggggagaag aatcaccttc
     3721 cgctctctga agcggacctt gacctaaaag ccctcttcaa agccttgcat gaatttggct
     3781 gtgggggacg tatcctctgc gaaagtccaa ttatggaaga ggatgccttg gtgatgaaga
     3841 agacgtggat gaaggttagc ggagagaaag agaaataata gggtttgttt ttgttatagt
     3901 aattacgatt gataattaaa cgtttagtct attaggtcta gtagtgttta aattttctga
     3961 gatcgcatgt aaagcaaaaa gtcgataaac acaatagtca attttgtttt aaatagagga
     4021 ataaagtgac aattacattg ccaatactag acctgttttt ttgtaccgta ttattttttc
     4081 tttttgcttt ttgtttttat caaggctggg aaatatgctt tatgcaaaag aatgagccat
     4141 tgtttcctta tcaagtaata gctttgatag caagttattt ggggaaagag gatgaattcc
     4201 gtgaaagaac atcacattta cgtacttcgt ttttattaag ggcacagggc gtttcagctt
     4261 tgttgggcgg agctttaggt atagttttat gtgttgtgtt ttttattaat attatttccc
     4321 gagtaataat aaatgagtga cctcttgtct tatgttttgg ataacatcta gagctaaata
     4381 taaaaagcgc cgctgaattc agcggcgctt ttacttacca aagcctactt acttattcaa
     4441 tccaacattc aactcaaacg aatctgggtc gctggagttc gcgatcatgt tatccgctga
     4501 ggcgtcgtaa tagccttggg cagcaaagac caaggtgaac tctttattgc gcgaaatatc
     4561 tgtgacctta tatttgccgt tcgagtctgt ttgcagatag gtttctatat atttatcggc
     4621 ataatcagaa ctttcccatt cgtcgtaggt tgtgccagag gtgagtacaa agacgtatgc
     4681 tccagaaatg ggattattcg ttgcggcatc atagacttgt ccgtagaccg agatggtatc
     4741 gccacttgat gaaggctgag attgttgacc gctattaccg cctccgctcg caacggtgac
     4801 cgtggatgaa ttgcccatgg gggtcatggc atctccggcg tacatttcga ggcgataggt
     4861 tccagcgggc atggggttgc catcgttcgg taggaaggtg ccgaagagtc cgctttcgcc
     4921 ccattcccaa gtgtagctat actcgccaac ttgttcatcg ttcaggaacc agcgttccac
//
LOCUS       DAOREF010000082         4972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9533_length_4972_cov_4.268660, whole genome shotgun sequence.
ACCESSION   DAOREF010000082 DAOREF010000000
VERSION     DAOREF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9533_length_4972_cov_4.268660"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PKI33_02620"
     CDS             complement(<1..918)
                     /locus_tag="PKI33_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35922.1"
                     /translation="MTDLNQAIQLIRQGQKFEAQRILQEIIKTEPKNIPAWFWYVETC
                     ATTERRIQVLETCLKMNPGNLQVEQALQTFRNSQPPVPAPPKVEPPTYQSNTPEPQKN
                     DDLYGYTSFRSDSFGQTQSYEEDKSPAYDSPLYEYETFRTNSSVKAETQKKQPWEMDL
                     STYEDNSMLAKSKKVRRSYSAFDVWLTALTSQDEKSYADLLEDPEAGLERAFSWVAIA
                     GLITALTVPVQYFLNPNLGELLDIPELQRQVGNMTPNAFIIILTMISFVTAPIGAILN
                     LALSGGIQNLLARFFGGGGNFKRTAYALSA"
     gene            complement(990..2822)
                     /locus_tag="PKI33_02625"
     CDS             complement(990..2822)
                     /locus_tag="PKI33_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNM35923.1"
                     /translation="MYGWHLRILRVNLTTRKVTTEDVDPQIARDYLGGRGWAIHYMYK
                     EMDPAVDPLSPENMLIFATGPLTATPAPTGNRYMVVTKSPLTGALAHSNSGGEFPTWM
                     KRTGFDMFIFEGKASEPVYVLVNEDQIEIKSAAHVWGKDTHETTDILKAETSDEARVA
                     CIGPAGENLALMAAIMNDKHRAAARSGVGAVMGSKNLKAVVAMGNKNPPLHDSEGMRA
                     ISVETSKNVGADVKKGSNMRIYGTSYVPQVTNTLGILPTRNFLQGTFEDVHNVDGDAL
                     KNQYLIRHTPCYRCPLSCGRLTEVPDGPYKGKGEGPEYETISSLGTGCGVSNLAALVK
                     ANYLCNEYGMDTITTGMTIAVAMEMYEKGYLTEETIGMPLKFGDHDAMIEMIKLMAYN
                     KGFGQDLAQGSYRLAEKYGHPELAVTTRKQEFPGYDPRGSQGMGLLYATSNKGASHME
                     GDVAYEEVFGVPIKENPLSTEGKPELVKHFQDSFALIDSSGLCVFVAIRYVFDKNRLI
                     WPQTLAAMMNLSTGAGYTPEEVMKAADRVYTLERMFLIKAGSGPEWDVLPYRMLHEPL
                     PDGPAKGKVAELDKMLPEFYEKRGWDEKGYPTQAKLMELGLPLN"
     gene            complement(2878..3117)
                     /locus_tag="PKI33_02630"
     CDS             complement(2878..3117)
                     /locus_tag="PKI33_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNM35924.1"
                     /translation="MLIRVKMIANYRDALPPGHKHGVIELDVPEGTTVYDAISRFDIP
                     LNDESVIVLNGLTVDMDTPLREGDMVTAFSAIAGG"
     gene            3179..3619
                     /locus_tag="PKI33_02635"
     CDS             3179..3619
                     /locus_tag="PKI33_02635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNM35925.1"
                     /translation="MRKKVPNHGPCFVCGSENPQSIGITMFVEEDGRLTSEFTLNEAH
                     QGPPGHAHGGASAAILDEVMGLVVWAAGHQVLAANININYRKPLPLHIPLLAEAHITE
                     VRERKIISVGKILLPDSTIAVEGSGVYVIAKHFFENSLLNGVHS"
     gene            3616..4311
                     /locus_tag="PKI33_02640"
     CDS             3616..4311
                     /locus_tag="PKI33_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNM35926.1"
                     /translation="MKTIGMIGGMSWESSIEYYRIINETAKEKLGGLHSAKSLMVTVD
                     FAEIEKLQHEDRWDEAGQILARCAQDLERGGADFIVLCTNTMHKLADQMTSNVSIPLL
                     HIADATAEKIVAAGIKRIGLLGTRFTMEHDFYKGRLIHNYGLDVLIPEKTDRDIIHRV
                     IYEELVLGKIVDASRREYQRIMNDLLAKGAEGIILGCTEIELLITEGDSSAPLFPTTK
                     IHAIAAVEKALAE"
     gene            4390..>4972
                     /locus_tag="PKI33_02645"
     CDS             4390..>4972
                     /locus_tag="PKI33_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35927.1"
                     /translation="MFNLFKKKEPKQSDPFLDMRTQFMGNVNFNEWPKGDSDAQPWSL
                     FVAARKAYSTNNHAEAEKYLRQVTETPDLEPRHYLQAWLFLRHFLKVQPPAEIAKKVY
                     GVMVEVGTPTGVMAVVGYTDHKARTLHSSGGGTVWERPNASMDDKVDALIKAGENAVQ
                     AIPLVVVDIVPAPPKQADHVLICIATPSGIYHGL"
BASE COUNT         1299 a         1173 c         1214 g         1286 t
ORIGIN      
        1 tgcagataat gcatacgctg ttcgtttaaa gttgccgccg ccaccgaaga accgcgccaa
       61 caaattttgg atgccacccg aaagtgcgag attaagaatc gctccaatag gcgccgtgac
      121 aaaagagatc atggtcagaa taataatgaa cgcattaggt gtcatattcc caacttgccg
      181 ttgcaattcg ggaatatcaa gaagctctcc taaattaggg ttgagaaagt attggacagg
      241 aactgtcaaa gccgttatta acccggcaat ggcaacccaa ctaaaagcac gttcaagacc
      301 tgcttctggg tcttccaata ggtcggcata tgatttttca tcctgagagg ttaaggctgt
      361 caaccataca tcaaacgcag aataactacg ccgaaccttt ttagacttgg ctaacatgga
      421 gttatcttcg taggtagaca aatccatttc ccaaggttgc ttcttctgag tctctgcctt
      481 cactgatgaa tttgtgcgaa atgtttcgta ctcatacaag ggcgaatcat atgctggaga
      541 tttatcttct tcataagatt gtgtctgccc aaaagaatcg gaacggaatg aggtgtaacc
      601 gtacaaatcg tcgttctttt gaggttcagg agtgttgctt tgataagtgg gcggttcgac
      661 cttgggagga gctggtacgg gcggttgaga gtttcgaaaa gtctgtaacg cctgctctac
      721 ttgaaggttg cctgggttca tttttagaca agtttcaaga acttgaatcc gtctttcagt
      781 ggtcgcgcag gtttccacat accaaaacca agcagggatg ttcttcggct cggtcttgat
      841 aatttcttgc agaatacgct gcgcctcaaa tttttgcccc tgacgaatga gttggatggc
      901 ttgatttaga tctgtcattc gaactcccaa gaaaaagata tgcccatagc ttgtgttgac
      961 caagctatgg gcaggttaaa acaactctgc taatttagcg gcaaacccaa ttccattaac
     1021 tttgcctgag tggggtaacc tttttcatcc cagccacgtt tttcatagaa ctctggcaac
     1081 atcttgtcga gttctgctac ttttccctta gcaggaccat caggcaaagg ttcatgcaac
     1141 atgcggtaag ggagcacatc ccattcggga ccagaccctg ctttgatgag gaacatgcgc
     1201 tcgagagtat aaacacggtc ggcggctttc atcacttctt caggggtata acctgcacct
     1261 gtggataaat tcatcatagc tgccaaggtc tgaggccaga taaggcggtt cttgtcgaat
     1321 acatagcgaa tggcaacgaa tacgcacaac cctgaggagt cgatcaaggc aaacgaatct
     1381 tggaagtgtt tgaccagttc tggttttcct tccgttgaaa gcggattttc cttgatcggc
     1441 acaccaaaga cttcttcata cgccacatcg ccttccatat gtgacgcgcc cttgttggag
     1501 gtggcataca acaagcccat accttgcgag ccacgtggat catagccggg gaattcttgc
     1561 ttacgagtcg tcactgccaa ctcggggtga ccgtactttt ccgcaaggcg ataactgcct
     1621 tgcgccagat cctgaccaaa gcctttgtta taagccatca gcttgatcat ctcgatcatg
     1681 gcatcatggt cgccgaactt aagcggcata ccaatagttt cttcggtgag atagcccttt
     1741 tcgtacattt ccattgccac agcaattgtc ataccggtgg tgatggtatc catgccgtat
     1801 tcgttgcaca gataatttgc tttgaccaat gcggcaagat tgctcacgcc gcaccccgta
     1861 cctaaagatg aaatggtttc atattcagga ccttctccct tgcctttata cggaccatca
     1921 ggcacttcgg tgaggcgccc acatgaaagc ggacagcgat aacaaggggt atggcgaatc
     1981 aaatattgat tcttcaaggc atcgccatct acattgtgaa catcttcaaa agtgccttgt
     2041 aaaaagtttc gcgtgggcaa aatgccgagg gtgttggtca cttgtggcac atacgaagtg
     2101 ccataaatgc gcatgttcga gcccttcttt acgtcggcac caacattctt ggaggtctcc
     2161 acgctgatgg cacgcatccc ttcagaatca tgcagaggcg ggtttttatt tcccattgcc
     2221 actacggctt tgagattctt acttcccatc acagcgccca cacccgaacg agccgccgca
     2281 cgatgtttat cgttcatgat ggctgccatc agtgcaagat tctcgcctgc aggtccgata
     2341 catgcgactc gcgcttcatc ggatgtttct gccttgagga tgtccgtcgt ttcgtgtgta
     2401 tccttgcccc acacatgcgc cgcggactta atttcgattt ggtcttcgtt caccaaaaca
     2461 taaactggct ccgaggcttt gccctcaaag ataaacatat cgaaccctgt gcgtttcatc
     2521 cacgttggga attcaccacc agagttagag tgtgcaagtg cgcccgtcag cggagatttg
     2581 gttaccacca tgtatcggtt gccggtcggc gcaggcgtgg ccgttagcgg accagtggca
     2641 aagatgagca tgttttctgg cgaaagcggg tccacggcag gatccatttc tttgtacata
     2701 taatggatcg cccatccgcg cccaccgaga tagtcgcgtg cgatttgtgg gtctacgtct
     2761 tcagtggtta cttttcgagt agttaggttt acgcgtaaga ttctgaggtg ccagccgtac
     2821 atgggaactc cttgaaaaag tttgaacgtt gaaatgtttc aacgattaga accgagatca
     2881 gccgccagcg attgcggaaa atgctgtgac catgtcaccc tctcttaacg gggtatccat
     2941 atccacggtg agaccgttca agacgatcac gctttcatca ttaaggggaa tatcaaaccg
     3001 agagatagca tcgtagaccg ttgtcccttc ggggacatcc aactcaatga cgccatgttt
     3061 atgccctgga gggagggcgt cacgatagtt ggcaatcatc ttgacacgga tcagcataag
     3121 gcgattctag cataatcagc caaagggtac agaaggtctt tgtggttaaa ttgacgttat
     3181 gagaaaaaaa gtaccgaacc atggtccctg ttttgtatgc gggagtgaaa acccgcaatc
     3241 cattggaatt acgatgtttg ttgaagagga tggaagacta acatctgaat tcaccttgaa
     3301 tgaggcacat caaggtccgc cggggcatgc ccatggcgga gcctcggcag ccatcttaga
     3361 cgaggtaatg ggcttggtcg tttgggcggc tgggcaccaa gtgctggcag caaacatcaa
     3421 catcaattat cgcaaacccc ttccgcttca tatccctttg ctggctgaag ctcacatcac
     3481 cgaggtgcga gaaagaaaaa tcatcagcgt aggcaaaatt ctgttgcctg attcaactat
     3541 tgccgtagaa ggaagcgggg tgtacgtcat cgcaaaacac ttttttgaaa attcactttt
     3601 gaatggagtg cattcatgaa aactatcggc atgattggtg ggatgagttg ggagtcgtcg
     3661 attgagtatt accgcatcat caatgagacg gcgaaagaaa agttaggcgg gttgcactcg
     3721 gcaaagagtt tgatggtaac cgtagatttt gccgagatcg agaaactgca acacgaagat
     3781 cgctgggatg aagctgggca aattttggca cgctgcgcgc aagaccttga gcgtggcggc
     3841 gcagatttta tagtgctatg cacgaacacc atgcacaagt tagcagacca gatgacttca
     3901 aatgtcagta ttccgttgct gcacattgca gatgcaacgg ctgaaaagat tgtggcggca
     3961 ggtataaagc ggattggtct gttaggcact cgctttacaa tggagcatga tttttataag
     4021 ggtcggctca tccataatta cggcttggat gtgctcattc cagaaaaaac agaccgcgac
     4081 attattcacc gcgtaattta tgaagagctt gtgcttggca agatcgtaga tgcatctcgg
     4141 cgtgaatatc aacgcattat gaacgatctg ctggcaaaag gcgccgaagg gattattttg
     4201 ggatgcaccg aaatcgaatt gcttattaca gaaggtgata gttcagcacc gcttttcccg
     4261 accacaaaga tccatgcgat tgcggcagta gagaaagcgt tggcagaata aagcgtttta
     4321 taaccaaatg tcctatttac cagcattgag aaatgcatat actccagcaa gatcatttct
     4381 ggagacatta tgtttaactt atttaaaaag aaagaaccca aacagtctga cccatttttg
     4441 gatatgcgta cccagtttat ggggaatgtt aatttcaatg aatggcccaa aggcgactcg
     4501 gacgcacagc cttggtcttt gttcgttgcg gcacgaaaag catattcgac aaacaatcac
     4561 gcagaagcag aaaaatatct gcgccaagtt accgaaaccc cagaccttga accacgtcac
     4621 tatttgcagg cttggttatt cttgcgtcac ttcttgaagg tgcaaccccc tgccgaaata
     4681 gccaaaaaag tatatggtgt catggtggaa gtcggcactc ccacaggcgt gatggcggtt
     4741 gtgggatata cagaccacaa ggcacgtacc ttgcactctt ctggtggcgg cacggtttgg
     4801 gaacgcccca acgcttcgat ggatgacaag gtcgatgccc ttatcaaagc gggtgaaaat
     4861 gctgtgcaag ccattccgct cgttgtggtg gatattgtcc ctgccccacc caaacaagcg
     4921 gaccatgtct tgatctgtat tgccacacct agcggaattt accacggact tg
//
LOCUS       DAOREF010000083         4941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9620_length_4941_cov_4.961113, whole genome shotgun sequence.
ACCESSION   DAOREF010000083 DAOREF010000000
VERSION     DAOREF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9620_length_4941_cov_4.961113"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..570)
                     /gene="tcmP"
                     /locus_tag="PKI33_02650"
     CDS             complement(118..570)
                     /gene="tcmP"
                     /locus_tag="PKI33_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNM35928.1"
                     /translation="MASLNLAFLDPNGLELEWSTIESLASVNKMDLIIYYSQSGLTRN
                     FENCINVDGDTDIDRFFGDREWRDIFKTSHGKSAGLHRKLIDQYKHKLSILGYLDVKD
                     IEDGAEPLMKNSKDAPLYRLIFASKHARGHDFWNKIIKKDAFGQGKLF"
     gene            complement(558..986)
                     /gene="tcmP"
                     /locus_tag="PKI33_02655"
     CDS             complement(558..986)
                     /gene="tcmP"
                     /locus_tag="PKI33_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNM35929.1"
                     /translation="MDKYLLPEDDGLPARTFGKWTTAKTDYLQRYVDLFETSMRGKPW
                     CARCYIDLYSGAGKYQVEGQTGFHLGSPLIGITTKYPFTHYFFADISQENIDILKQRC
                     EPASQIRKKFYVRDANLLAQEIVNEIKSIERNRSKEQWPL"
     gene            1071..2618
                     /locus_tag="PKI33_02660"
     CDS             1071..2618
                     /locus_tag="PKI33_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNM35930.1"
                     /translation="MPEPKRPLKVFLCHASTDKPKVRELYRYLKKRGIQPWFDEEDLV
                     GGQDWQVEIPKALATSDAIVICLTKNSVDKEGYIQKEIKFALDKALEMPEGRIFLIPV
                     KFEECEVPFTLSRYQWVDLTVESGYAKMMKALKFRASQLERSTVEISKKDVEEEYLVL
                     GKTDRKSNEPVEQKSKEKRSPEQQIYETIKKPRQKLTIRKLFGNTVLIFWSIFVFGAI
                     ALMWKPFFQMIAPATEEKTPQVATTSPYTETVAASTLESTIKPTLLPTEITDAKGVSM
                     VLVPAGEFSMGIDNSNNGNQDANLSHIVYLDDYYIDKYEVTNAQYKTCVEIGACTLPE
                     FTNSSTRSNYFDNPNYNDYPVVNVSWLQADTYCSWRKVRLPTEAEWEKAARGIDGRTY
                     PWGEDLNCINTNYSGCAGNDTVAVGSFLNDKSPYGAYDMGGNVEEWVWDWYSATYYQS
                     SPYANPLGPEDGENRVVRGGAWVWGSVSTFYNSLEPLKVFTRHWWYSTDQGYEHIGFR
                     CASDVVP"
     gene            complement(3043..3249)
                     /locus_tag="PKI33_02665"
     CDS             complement(3043..3249)
                     /locus_tag="PKI33_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNM35931.1"
                     /translation="MKQNRDLVYLGDILDAIRRIEDYTAGIKKKEFMEHLMMQDAVMR
                     QIEIIGEASNSVSDDFQEKHPELP"
     gene            complement(3236..3592)
                     /locus_tag="PKI33_02670"
     CDS             complement(3236..3592)
                     /locus_tag="PKI33_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNM35932.1"
                     /translation="MAQISAKPKTNKSTMPDKHRFVVLKTKVTPLLKPYASRISVFGS
                     YARGDMTASSDIDILIALKPAKSRPPLGLFGFIALEQNLAKKLGRPVDLVTEESINPR
                     RRKSIQQDRVVLYEAK"
     gene            3715..>4941
                     /gene="glyQ"
                     /locus_tag="PKI33_02675"
     CDS             3715..>4941
                     /gene="glyQ"
                     /locus_tag="PKI33_02675"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNM35933.1"
                     /translation="MTNSSFQEIILKLQNFWASKGCLITQPYYTQVGAGTMNPATFMR
                     VLGPEPWNVAYVEPSVRPDDGRYGENPNRLQKHTQFQVILKPDPSNPQELYLDSLKAL
                     GIDPRQHDIRFVEDNWEQPAIAAWGLGWEVWLDGQEITQFTYFQQMGGVALNPVSVEI
                     TYGLERILIALNNAKGIWNEDFGAGVTYGEIFRREEFEHSKYYYEVADIERTRQMYEL
                     FSAEADACLAQGLVVPAHDYVLKSSHTFNILDARGAISVAERQAFFRRMRELARQVAE
                     GYEAQRKELEYPLLKDAKNETRNSTTRPSNIEASNIASSFLLEIGVEELPASDVDTAY
                     EALSARVPTLLDELHLAHGEVRIFTTPRRLVVSVASLSPRQPDREDLVKGPPADKAFD
                     KDGIALPAGMGFAKKNG"
BASE COUNT         1330 a         1197 c         1067 g         1347 t
ORIGIN      
        1 aaaggattcc aagtggattc tgtccactcg attcctgaat gttcagccat tcttcctcct
       61 ttgaagaatc tacttgtaaa gtttgtttac ataataatag cacgaatttt ctattttcta
      121 gaatagtttt ccttgcccaa aagcatcctt ctttatgatt ttgttccaga aatcatgtcc
      181 tcttgcgtgt ttgctggcaa aaataagacg gtacagcgga gcgtctttgg agttcttcat
      241 taatggttct gcgccatctt caatatcctt gacatcgaga taaccaagaa tggatagttt
      301 atgtttgtat tgatcaatca gtttcctgtg taaaccagcg ctcttcccgt gagatgtttt
      361 gaaaatatcc cgccattccc tgtccccaaa aaagcgatca atgtcagtat cgccatctac
      421 attaatacaa ttttcaaagt ttctcgtcaa cccactttgt gaatagtaaa ttattaaatc
      481 cattttattc accgaagcaa gagattcaat tgttgaccat tctaattcca gcccattggg
      541 gtcaagaaat gccagattta aagaggccac tgttcttttg agcgatttcg ctcaatggat
      601 ttaatttcat taacgatttc ttgagcaagt aaattggcat cccttacata aaatttcttt
      661 cttatttgac ttgcgggttc acaacgttgt ttcaaaatat cgatattttc ttgcgaaata
      721 tccgcaaaga aataatgggt gaaaggatat ttggttgtaa ttccaattaa tggggaacca
      781 agatggaaac cagtttgtcc ctcaacttgg tactttcctg ctcctgagta aaggtcaata
      841 taacatcttg cgcaccaagg tttaccgcgc attgaagttt caaagagatc aacatatctt
      901 tgaaggtaat ctgttttagc agttgtccat ttaccaaaag ttcgagcagg taaaccatca
      961 tcttcgggaa gtaaatattt atccattttc cactcctttt ttgtcggata gtgtgagtat
     1021 agcagattct gccgtataat ttcactaatt cacagcggga aacccttcct atgcctgaac
     1081 ccaaacgccc tctcaaagtc ttcctctgcc acgcatccac ggacaagccc aaagtccgtg
     1141 aactctatcg ctacctaaaa aagcgaggga ttcaaccgtg gtttgacgaa gaggacttgg
     1201 tgggtgggca ggactggcaa gttgaaatcc ccaaagccct ggcaacttcg gacgccatcg
     1261 tcatttgttt gaccaagaac tcagtagata aagaaggcta catccaaaag gaaatcaaat
     1321 ttgccttgga taaagccctc gaaatgccgg agggacggat tttcctgatt cccgtcaagt
     1381 ttgaggaatg tgaagtccca tttaccttga gccgttatca atgggtggat ttgacggttg
     1441 aatctggcta tgccaagatg atgaaggcgc tgaagttccg tgcttcgcag ttggaacgat
     1501 caacggttga gatttccaag aaggatgttg aagaggaata tcttgtcctt ggtaagacag
     1561 atcgcaaatc caacgagccc gttgagcaga aatcaaaaga aaaaagatca cctgaacaac
     1621 aaatttatga aaccattaag aaaccaagac aaaaactaac aatcagaaaa ctttttggta
     1681 atacagttct tatattttgg agtatttttg tatttggcgc aatagctttg atgtggaagc
     1741 cgttctttca gatgattgcc ccagcaaccg aagaaaagac gcctcaagtt gctaccactt
     1801 caccatatac agagacagtt gctgcatcaa ccttagagtc tacgataaaa cctacacttt
     1861 tgcctaccga aatcacggat gccaaaggtg tgtcaatggt gcttgtacca gcaggtgagt
     1921 tttcaatggg aattgacaat agtaacaatg ggaatcagga tgctaatctt tcacatattg
     1981 tttatttgga cgattattac attgataaat acgaagtaac aaatgcacag tacaaaactt
     2041 gcgttgaaat aggagcatgt actctcccag aatttacgaa ctcatctaca cggtctaatt
     2101 attttgacaa cccaaactat aatgactatc ctgtagtgaa tgttagttgg ttacaggctg
     2161 atacttattg tagttggcgg aaagttagat tacctacaga ggcagaatgg gaaaaagcag
     2221 cccgcggtat tgatggacga acatatcctt ggggggaaga ccttaattgt attaacacta
     2281 attattctgg ttgtgcgggg aatgatacag ttgctgttgg aagttttcta aatgataaaa
     2341 gtccatatgg tgcatatgat atgggtggta atgtggaaga atgggtttgg gattggtact
     2401 ctgcaacata ctatcaatct tccccttatg ctaacccttt aggaccagaa gacggagaaa
     2461 atcgagttgt acgtggtgga gcttgggttt gggggagtgt ttcaactttc tataatagtt
     2521 tggaaccatt aaaagttttt acgcgtcatt ggtggtattc tactgaccaa ggctatgaac
     2581 atattggttt ccgttgcgct agcgatgtag tcccataaaa tagactactt ggtattattc
     2641 caatatcttg taaaacctat agcgcaccat aagatggagg gtgaatcccc attaaccatc
     2701 agttataatc cccccgcaat caccaatcca aaatcggcaa tcggaaatca aatgtcttct
     2761 tccttccaat ccatcatcct caaactccaa gacttctggg catcgcacgg atgtctcatc
     2821 acgcagccct actacaccca ggtcggtgca gggacgatga ataaaagaac cccgagattt
     2881 tcaaaatctc ggagttctca tttttaccta ctactcccct aaaatctctt caacctgttt
     2941 cttgagggcg ggcagatcat tcttcaccgt atcccaaatg acctgtaaat taattccccg
     3001 ttagtcatgg atgatcttat tgcgtatccc gcgcatttga aactacggca gttcagggtg
     3061 cttttcttga aaatcatctg aaacactatt ggacgcttcg ccaatgatct ctatttgccg
     3121 cattacggcg tcctgcatca tcagatgttc catgaattcc tttttcttta taccggcagt
     3181 gtagtcttca attcggcgaa tagcgtccaa aatatcaccg aggtagacca agtccctatt
     3241 ttgcttcata aagtactacc ctgtcttgtt gaatactttt tcttcggcgt ggattaatac
     3301 tttcttctgt caccaagtcc acagggcgtc ccagtttttt tgctaggttt tgttccaggg
     3361 caatgaaacc aaacaacccc aatggtgggc ggctcttagc aggtttcagt gcgattaaaa
     3421 tgtcaatatc gctagaagca gtcatgtctc cgcgggcata cgacccgaaa acagaaattc
     3481 ggctggcgta tggtttcaac aatggggtaa ctttcgtttt caaaaccaca aaacgatgct
     3541 tgtcaggcat cgtacttttg tttgtttttg gttttgcgct tatctgagcc atataaatat
     3601 tataccgaat ttcttaacct gccatccatt ccccatcagt tataatcccc ccgcaatcat
     3661 caatccaaaa cttgtgctga gattgtcgaa gcatcccaag atcggaaatc gtacatgacg
     3721 aactcctcct tccaagaaat catcctcaaa ctccaaaact tctgggcatc caagggatgt
     3781 ctcatcacac agccgtacta cacccaggtc ggcgcaggga cgatgaaccc ggccaccttc
     3841 atgcgcgtgc tcggccctga gccgtggaat gtggcttatg ttgagccgtc cgtccgcccc
     3901 gatgacgggc gctatggcga gaatcccaac cgtttgcaaa agcacaccca attccaggtg
     3961 atcctcaagc cggatcctag caacccgcaa gagttgtatc tcgactcgct caaggccctc
     4021 ggcatcgacc cgcgccagca tgacatccgc tttgtggaag acaactggga acagcccgcc
     4081 atcgccgcat ggggcctcgg gtgggaggtc tggctcgacg ggcaggagat cacgcagttc
     4141 acctacttcc agcagatggg cggcgtggcg ctcaaccccg tttcggtcga gatcacctac
     4201 ggtcttgaac gcatcctcat cgcgctcaac aacgccaaag gcatctggaa cgaagacttc
     4261 ggcgcgggcg tcacctacgg cgagatcttc cgccgcgagg aattcgagca ctccaaatat
     4321 tattacgagg tcgccgacat cgagcgtacc cgtcagatgt acgaactctt ctccgcagag
     4381 gccgatgcct gtctcgcgca ggggcttgtc gtccccgcgc acgattacgt gctcaagtcc
     4441 tcgcatacct tcaacattct cgacgcgcgc ggcgcgatca gtgtggccga acgtcaagct
     4501 ttcttccgcc gtatgcgcga actcgcccgt caggtcgccg aaggctacga agcgcagaga
     4561 aaagagttgg aatatccgtt gttgaaggac gcgaaaaacg aaactcgaaa ctcgaccacc
     4621 cgaccatcga atatcgaagc ctcaaatatc gcttcctcct tccttctcga gatcggcgtg
     4681 gaagaactcc ccgcttcgga tgtggatacc gcctacgaag ccttgtctgc ccgtgtgccg
     4741 accctgctcg atgaacttca tcttgcgcat ggcgaggtcc gcatctttac gaccccgagg
     4801 cggctcgttg tttctgtggc ctctctctcg cccaggcagc ccgaccgcga agacctcgtc
     4861 aaaggtccgc ccgccgataa ggccttcgat aaagacggca tcgccctccc ggcgggtatg
     4921 ggttttgcca agaagaacgg c
//
LOCUS       DAOREF010000084         4935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9637_length_4935_cov_3.732582, whole genome shotgun sequence.
ACCESSION   DAOREF010000084 DAOREF010000000
VERSION     DAOREF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9637_length_4935_cov_3.732582"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..869)
                     /locus_tag="PKI33_02680"
     CDS             complement(114..869)
                     /locus_tag="PKI33_02680"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNM35934.1"
                     /translation="MTFNRAKNFLFRNRHNLAVFVLLNLACVACIVFVAARVVYTDSS
                     RHTGLIWNLFLAWIPFILAYFAHALSWKKVWVYLAIPFVAFLWLLFFPNAPYMLTDLQ
                     DLARGTGYEAPLWYDVIIVVWCSWTGTLLGVISLYLMQDIIARTFHRWLGWVFVFAIS
                     GLSSFGIYIGRFVRLNSWDILQNPAETAMEILGIVIDPSKRLAAFTLAYTFFFLFIYL
                     LLYSFSHMLQEQVIRAQSSPEQLASTQPTQPAH"
     gene            complement(866..1360)
                     /locus_tag="PKI33_02685"
     CDS             complement(866..1360)
                     /locus_tag="PKI33_02685"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM35935.1"
                     /translation="MNKEYIQTLYRYNQWSNARILDTAAKLSPAQFLATSEHPHRYLR
                     GVLTHTLFAEWIWHKRWTGESPTTHLKPEDFPTFEALRTRWAEEESQLMQFVAQVTDE
                     KLNAPFTYLATDGTKYENILWESMAHVVNHGMQHRSEAAAILTELGVSPGDLDFILFT
                     RKKL"
     gene            complement(1412..2287)
                     /locus_tag="PKI33_02690"
     CDS             complement(1412..2287)
                     /locus_tag="PKI33_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM35936.1"
                     /translation="MPFPYLDETLLLNAETRKDLPGSFITLPSGVTHYEEGGDPKGQP
                     VVLVHGFSVPYFIFEPTFVFLCQQGFRVLRYDLLGRGFSDRPNTRYNATLFVHQLKEL
                     LDALNYKNICLAGLSMGGAVTSSFIDAYPDYVSKYILIDPSGARAVSLSPFLKAVRMP
                     GFGELAIGLFGSDSMIKGIASDLFSPELVREFQEKYKVQMQYQGFKRAILSTMRNGML
                     DSFIETYKRVGKLKKPTLLFWGKQDTTTPFEHSADICAVMPHAEFYAFDSCGHIPHYE
                     RASEVNRILLEFLRK"
     gene            complement(2348..3691)
                     /locus_tag="PKI33_02695"
     CDS             complement(2348..3691)
                     /locus_tag="PKI33_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNM35937.1"
                     /translation="MPDQIYVVGHINPDTDSIASAVGYAWLLRERDGLNTIAARAGAL
                     NAQTSWVLKTLGLESPFLLTDASPRFDSVMQRLDSLRPNAQLGSAWTLASRTGGLAPV
                     VNEDGTPYGIISGMSLFKYFSNILGPRPGDTTVREMMSAECKDAADTSVPKYQANAHI
                     RDSLNRILRDEIDDYWVVDESGRYLGIARQRNLLNPPRLKIILVDHNEPRQSIAALEE
                     AELLEILDHHRLGNPYTHQPIRFTVDVVGSTSTLVTEQTAEAGLSAPPAIAGALLSGL
                     LSDTLILTSPTTTDRDRVAAERLARWAFVGGSPLQGETIETYGKAVLSAGAGLSNRKP
                     EEVVSTDIKPFEGGGFKFAVAQAEVTDIMQLSEHLAPLTKALDDLRDKRGLDFALLLV
                     TDVVRGTSRLIVTSSAPPILNDLPYPPLPDGTRDAPGVVSRKKQLLPAVLGLLER"
     gene            complement(3733..>4935)
                     /locus_tag="PKI33_02700"
     CDS             complement(3733..>4935)
                     /locus_tag="PKI33_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNM35938.1"
                     /translation="IALWSGQLVFKRLIQPQSLYRAQALIVLLGFAIPLTSWTLTLLG
                     VQITPERDATPFSAAIGNLIIAWGIFRFHIFEVIPIARDRVFEALADPVVILDKNNIV
                     IDINAAMLTLMDKREHEVIGNPAKDVFNDFPIPIKLYTHVSYARTEAVFEISGKQIHY
                     DLTIWPLYNTNKEMVGRVYVSHDITALKELEAELRKLNVQLEDRVRDRTRELAEAYES
                     MLEGLALALELRDKETEGHSRRVTENAIKLAQKMGIAGSALEDIRSGAILHDIGKISI
                     PDEILHKPGKLTLDERAVVEKHPETAFKLLSRIPFLSQAIEIPYCHHEKWDGTGYPRK
                     LAGEDIPLSARIFAVADVWDALSYDRPYNKAWTREQIIEYFNEQTGKHFDPAITKIFL
                     SMVEKGEI"
BASE COUNT         1279 a         1137 c         1261 g         1258 t
ORIGIN      
        1 gacgaaaacg aaacggtctt agtttgttat atattttttg aaacatggtg tctccgggtg
       61 tttgacccat tgtaattagg cgggcttcca aatccttgca aagactagca ggcttaatgc
      121 gctggttgtg ttggctgcgt agaagccaat tgctccggag agctttgagc acggatcacc
      181 tgttcttgca gcatgtgact aaatgaatac aacaacaggt aaatgaacaa gaagaaaaag
      241 gtatatgcca acgtaaaggc ggcgagacgc ttgcttgggt caatgacgat gccgagaatt
      301 tccatggcgg tttcggcggg gttttgcaaa atgtcccatg aattcaggcg cacgaagcga
      361 ccgatgtaga tgccaaaact gctcagccct gaaattgcaa agacgaagac ccatcccagc
      421 cagcggtgga aggtgcgggc gataatgtct tgcatcaaat agagcgagat gacgcccagc
      481 aaagttcctg tccacgagca ccagacgacg atgatgacat cgtaccaaag cggagcttca
      541 taacccgtgc cgcgagcgag gtcttgcagg tcggtcaaca tgtatggagc gttggggaag
      601 aacaatagcc acagaaaagc cacaaatgga attgccagat atacccaaac ttttttccac
      661 gaaagagcat gagcgaaata cgcaaggatg aacggaatcc atgccaggaa gagattccaa
      721 atcaaacccg tgtgacgcga cgaatcggta tacaccacac gcgctgctac aaaaacaata
      781 caagccacgc atgccaggtt aagcaagacg aacacagcta ggttatgacg attccgaaaa
      841 agaaaattct tggcgcgatt gaaggtcata gtttctttct tgtgaatagg atgaaatcta
      901 aatcaccggg agaaacccca agttcggtga gaatggcagc ggcttcactg cgatgttgca
      961 tgccatgatt gaccacatgt gccatcgact cccacagaat attttcatat tttgtgccat
     1021 cggtggctaa ataggtaaaa ggcgcgttca gtttttcgtc cgttacttgc gcaacgaatt
     1081 gcattaattg gctttcttcc tctgcccaac gtgtacgcaa tgcttcgaag gttggaaagt
     1141 cttccggctt caagtgcgtg gtgggcgatt cgcctgtcca gcgcttgtgc caaatccatt
     1201 cggcaaagag ggtgtgtgta aggacaccgc gtaaatagcg gtgtggatgt tctgaagttg
     1261 ctaaaaattg cgcagggctg agtttggcgg cggtgtcgag aatccgcgca ttcgaccact
     1321 ggttatagcg gtacaacgtt tgaatatatt ctttgttcac ggttgctcct ttaatccatc
     1381 ccccaatcac tatgtatttg ttttgaggga attacttcct caaaaattct aacagaattc
     1441 tgtttacctc gcttgcgcgc tcgtagtgag gaatgtgccc acaactgtca aaggcataga
     1501 attctgcatg cggcatcact gcgcatatat cggcactatg ctcaaatgga gttgttgtat
     1561 cttgttttcc ccaaaaaaga agagtcggtt ttttgagttt gccaactcgt ttgtaggtct
     1621 caataaaaga gtccaacatg ccattgcgca tcgtagaaag aatggcacgc ttgaaccctt
     1681 gatattgcat ttggacttta tatttttctt ggaattcacg cacaagttct ggactgaaca
     1741 agtctgatgc aatgcctttg atcatgctgt cgcttccgaa cagaccgata gccagttcgc
     1801 caaacccggg cattctcacc gctttgagaa aaggcgagag cgaaactgcc cttgcgccag
     1861 atgggtcaat gagaatatat tttgaaacat aatcagggta cgcatcaata aacgaagagg
     1921 tgaccgctcc acccatagat agccctgcaa gacagatatt tttgtagttc agtgcatcca
     1981 acagttcttt gagttggtga acaaacagag ttgcgttgta tcttgtattt ggacgatctg
     2041 aaaacccacg cccaagcaag tcatagcgca agacacgaaa gccttgctga caaagaaata
     2101 caaaagttgg ttcgaaaata aagtaaggga cagaaaatcc atgcaccaaa actaccggct
     2161 gaccctttgg atccccgcct tcttcgtagt gtgttactcc gctaggcagg gtgatgaacg
     2221 agcccggcaa gtctttacgg gtttcggcgt tgagtaaaag ggtttcgtcg aggtatggga
     2281 agggcatggt ttttcaaaat tatacaaaat aaggcgggtt caaaacccgc cctatatgga
     2341 catttactta tcgttctaat aaacctaata cggctggcaa caactgtttc tttctcgaaa
     2401 ccactccggg tgcatcgcga gtgccgtcgg gtaagggcgg gtagggaagg tcattgagaa
     2461 tgggtggtgc acttgatgta acgatcaagc gactggttcc acgcaccaca tcagtgacca
     2521 gtaacagcgc aaagtcgagt ccgcgtttgt cgcgtaggtc atcgagggct ttggtgagtg
     2581 gtgcaaggtg ttctgaaagc tgcatgatgt cggtcacctc ggcttgagcc actgcaaatt
     2641 tgaatccgcc gccttcgaat ggtttgatat ctgtgctgac gacttcttct ggcttgcggt
     2701 tggaaagccc agcacctgca ctaagaacgg cttttccata ggtttcgatg gtttcgcctt
     2761 gtagggggct gcctccaaca aacgcccagc gtgccaaccg ttctgcggcg acgcggtcac
     2821 ggtctgtcgt ggtgggagag gttaggatga gcgtatccga caaaagtcca gaaagaagcg
     2881 cacccgcaat ggcaggcggg gcagacaagc ccgcttcagc cgtctgttcg gtgacgagag
     2941 tcgaagtaga tcccactaca tccaccgtga agcgaatggg ctgatgggta taagggttgc
     3001 caaggcgatg gtgatcgaga atttccaaaa gctcggcttc ttcaagggca gcaatagact
     3061 ggcgcggttc gttgtgatcc acgaggatga tcttgaggcg cggcggattc agcaaattgc
     3121 gttgacgggc aatgccgaga tatcttccgc tttcatctac gacccaataa tcatcgatct
     3181 catcgcgcag aatgcggttg agcgaatcgc ggatgtgggc gttggcttga tattttggca
     3241 cactggtatc tgcagcatct ttacattcag ctgacatcat ttcgcgcaca gttgtatcgc
     3301 cggggcgcgg accgaggata ttgctgaaat atttgaacaa gctcatgccg ctgatgatgc
     3361 catacggggt gccatcttca ttgacaaccg gggcaagccc gcccgtgcgg ctggcgagag
     3421 tccatgcgct gccgagttgg gcgttgggac gtagactatc gagcctttgc ataacggagt
     3481 caaagcgggg tgaagcatcg gtcaataaaa atggagattc cagcccgagt gttttcaata
     3541 cccatgaagt ttgtgcgttt aaggctccgg cgcgggcggc tatggtgtta agcccgtcac
     3601 gttcgcgcag taaccaggca tatcccacgg cagaggcgat cgagtcggta tcggggttga
     3661 tgtgtcctac tacatatatc tgatctggca tttgttacat ctttctttac gtattttatt
     3721 ttgcggcacg gattatatct cgcctttttc aaccatggag agaaatattt ttgtgatcgc
     3781 agggtcgaaa tgtttgccgg tttgctcgtt gaaatattca atgatctgtt cacgagtcca
     3841 ggctttgttg taagggcggt cgtagctcag ggcatcccaa acatcggcaa cagcaaagat
     3901 acgtgccgaa agtggaatat cttcgcctgc tagcttacgc ggataccccg tcccatccca
     3961 tttttcatgg tggcagtatg gaatttcgat tgcctgactt aaaaaaggaa tacgcgataa
     4021 aagcttgaat gccgtttctg ggtgtttttc aactacggct ctttcatcca aggtcaactt
     4081 cccgggtttg tgcaaaattt cgtctggaat gctgatcttg ccaatatcgt gcagaatggc
     4141 gccactgcga atgtcctcaa gcgcgctacc tgcgataccc attttttgtg caagcttaat
     4201 ggcgttttct gttacacgcc tggagtgacc ttcagtttct ttgtctctta attcgagcgc
     4261 cagcgcgagc ccctccaaca tggattcgta cgcttctgcc aattcgcgcg tccggtcgcg
     4321 gacacggtct tccaactgca catttaactt tcgcagctca gcttccaatt ctttcaaggc
     4381 agtaatatca tgtgaaacat aaactcgccc gaccatttcc ttattggtgt tgtataaagg
     4441 ccagatcgta agatcgtaat ggatttgctt gccgctaatc tcgaaaacag cctctgtcct
     4501 cgcataagag acatgagtgt acagtttgat aggaatcgga aaatcattga aaacgtcttt
     4561 ggcagggttt ccgataactt catgttccct tttgtccatc aaggtaagca tggcggcgtt
     4621 gatatcaata acaatattat ttttatcgag aataaccaca gggtctgcca atgcttcaaa
     4681 gaccctgtct ctggcgatgg ggattacttc gaaaatatgg aaacgaaaaa taccccaggc
     4741 aatgatcaaa ttgccgatgg cagcactaaa cggggtcgca tctctttcgg gtgtgatttg
     4801 aacgcctagc aaggtgagcg tccaacttgt aagtggaatc gcgaaaccaa gaagaacaat
     4861 cagggcttgt gcgcgatata agctttgagg ttgaatcaga cgcttgaata ccaattgccc
     4921 agaccaaagg gcaat
//
LOCUS       DAOREF010000085         4926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9664_length_4926_cov_3.873127, whole genome shotgun sequence.
ACCESSION   DAOREF010000085 DAOREF010000000
VERSION     DAOREF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9664_length_4926_cov_3.873127"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKI33_02705"
     CDS             <1..812
                     /locus_tag="PKI33_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNM35939.1"
                     /translation="PAEEAGLLPGDKIVAIDGEDMTGIDGELVRQKVLGPAGSTVVLT
                     IMRDGEDKPLDFSIVRAQITIASATGKMLDGDIAYFQVTTFGSNTMPELEAALAEVMP
                     QKPKAIILDLRNNGGGYLQTAVEVASQFMGEGVVLYEQYGDGTRNTFDVLPGGKATDT
                     SIPMIVLINEGSASASEIVAGALQDTGRAKLLGTVSYGKGSVQNWIPLSNDNGAVRIT
                     IAKWLTPNERTIHKIGLTPDYPVDLTPEDRQADKDPQLDEAVRLLLEMLAK"
     gene            839..1531
                     /locus_tag="PKI33_02710"
     CDS             839..1531
                     /locus_tag="PKI33_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNM35940.1"
                     /translation="MFFLSPTYLMYMIPAFILMALTSWYVKSAYAKWSRVAAGSRFSG
                     YEATRRLIASSGLQGVKIQGTPGEMTDHYDPRDKTVYLSNGVANSASVAALAIAAHEL
                     GHAQQDAEEYFPLKFRGVLVPLVNIGSNLGWILIMIGLFLQLTQLAWLGILVFSGGAL
                     FALVTLPVEFDASARARRMLADSGIIQTDAEMRGVSNVLNAAALTYVAGLVTAVLQLL
                     YYVSLVSGRSRD"
     gene            1705..2469
                     /locus_tag="PKI33_02715"
     CDS             1705..2469
                     /locus_tag="PKI33_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35941.1"
                     /translation="MNRSLNIQLLPLLKKMSLRRGAIFGGLILCALLAFEVFNFGTTS
                     FALRDVLGDLQFAGMYWSSVLAFAFCAIDFAGIARIFTPEQGRDEPAEVWYLFGAWLL
                     AAAFNAMLTWWGVSVAIRNHNALGGGLVGQETMMTVVPIFVACMVWLIRVLLIGTFSV
                     AGERLFSMADIYDRNTAYRNVPASYSTPQPRTVQPAPRQEVNPPVNNYPRPVPKPQPR
                     PNPMPAYGANRPEPTYHPVGMSAMSRNENSNPSVRR"
     gene            complement(2561..3439)
                     /locus_tag="PKI33_02720"
     CDS             complement(2561..3439)
                     /locus_tag="PKI33_02720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM35942.1"
                     /translation="MSLPNIRLLETPDEMKLVEDLQRQVWPGSETDVVPSHVLITVVH
                     NGGLVMGAFEEEQLIGFVFGFPGLERTADGPRPKHCSHQMGIHPKYRDSGIGFELKRA
                     QWQMVRHQGLDHITWTFDPLLSRNAHLNISKLGAVCSTYRESEYGELRDGLNAGLPSD
                     RFQVDWWINSRRVEKRLSKRPRPTLKLAHVARSGLRPFYTPQFSDAGLMLAPEHVPPF
                     EDRLLLVEIPTNFMQIKEKDFPLARDWRFFSRELFETAFAKNYIVTDFVFDANEGSPR
                     GMYVLTHGDSTLDEFE"
     gene            complement(3441..4391)
                     /locus_tag="PKI33_02725"
     CDS             complement(3441..4391)
                     /locus_tag="PKI33_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM35943.1"
                     /translation="MILITGATGFIGRALVRQLSSIGYPLRALIRPSAKTPRLPKGVP
                     VEVAVVSLADTRGLRAAMRDVETVFHLASAENQGARGSLLITDIQGTQNLVEAAADAG
                     VDRIVYLSHLGAARASGYPAFKAKGIAEESIRAGKVPYTVIRTSLVYGPEDHFTNNLA
                     RLLRNSPGVFPVPNGGRVVVQPVWVEDLVTCMLWALQNESTVNQVYEVGGNEFFTLQQ
                     IIEIIMDVTRRRRFLVPLSPITLRAMTVFLESVVPGFPLSSLFLDYFAVNRTTAVDAM
                     PRHFGLMPARFSYRIDYLIYKRWYQRLWSAVTERLSPQRR"
     gene            complement(4491..4766)
                     /locus_tag="PKI33_02730"
     CDS             complement(4491..4766)
                     /locus_tag="PKI33_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35944.1"
                     /translation="MNNKNTGLIATIAAVMLCGCPGLFMCLFGAITATGNGTFNESSL
                     PPAVGIALVCVALIFILIPVGVGFFTMRKKPEAAAAPVSNDQTPPTA"
BASE COUNT         1211 a         1317 c         1225 g         1173 t
ORIGIN      
        1 ctccggctga agaagcaggt ttattacccg gcgataagat cgttgccatt gatggtgaag
       61 atatgaccgg tatcgacggg gaactcgtcc gccagaaagt gctcggacct gccggttcca
      121 ccgtggtgct gacgattatg cgtgatggcg aagacaaacc actagacttc tccatcgtcc
      181 gcgcccagat caccattgcc tctgccactg gcaaaatgct tgatggtgat atcgcttact
      241 tccaagtgac gaccttcggc tcaaacacca tgcccgagtt ggaagccgcc ctcgctgaag
      301 ttatgccgca gaaaccgaaa gctatcattc tcgacctgcg taacaatggt ggcggttatt
      361 tacaaaccgc cgtggaagtt gcctcgcaat ttatgggcga aggcgttgtg ctctacgaac
      421 aatatggaga tggcactcgc aacacctttg acgtactccc tggcggaaaa gcgaccgata
      481 ccagcattcc catgatcgta ttgatcaacg aagggtctgc ttcggcttct gagatcgtag
      541 ctggcgcatt gcaagatacc ggtcgcgcca agttgcttgg aaccgtctcc tatggaaaag
      601 gctccgtaca aaactggatt ccgctttcaa atgacaatgg agccgtccgc attaccatcg
      661 caaaatggct cactccgaac gaacgcacca tccataaaat cgggcttacc ccagattacc
      721 ctgtagacct tacgcccgaa gaccgtcagg cagataaaga cccacaattg gatgaagctg
      781 tacgcctcct cttggagatg ctcgccaaat agcttaatca actagaaagg agaactgaat
      841 gtttttcttg agtcccacgt acttaatgta catgatcccc gcctttattt tgatggcgct
      901 tacatcatgg tatgtcaaat cggcatatgc aaaatggagc cgagtcgcag caggcagccg
      961 cttcagcggg tacgaagcca cacgtcgcct aatcgcctcc agcggtttgc aaggcgtgaa
     1021 aatccaaggc actcccggcg aaatgaccga ccactacgac ccgcgcgata agactgtgta
     1081 tctttcgaat ggcgtcgcga acagtgcttc tgtagcggct ttggcaattg ccgcacatga
     1141 gcttggtcat gcccagcaag atgcagaaga atattttccg ctcaagttcc gcggcgtgct
     1201 ggtaccgctt gtcaatatcg gctccaatct cggctggatc ctcatcatga tcggcttgtt
     1261 cctgcaattg acacagctcg catggctcgg gattttggtc ttctcaggcg gtgccctttt
     1321 tgcactcgtc accttgcccg tggagttcga cgcctctgcc cgcgcccgac gcatgttagc
     1381 cgacagcggc attatccaaa ctgacgccga aatgcgtggt gttagcaatg ttctaaacgc
     1441 cgccgcattg acctatgtgg caggtttggt caccgccgtt ttacagctgc tgtattacgt
     1501 ctcactcgtc agcggacgca gccgcgatta agtttatgca atatcaatcg gggcggcttg
     1561 caagccgccc cgatattttt ttgggggata tccattcatg caattctcct gcaatccaac
     1621 gttaacttga caaattagaa caagtgttct atatttaact tcagataact tgacccgttt
     1681 ttggttccaa aaaggagaat tcccatgaac cgttccctca atatccaact tctgcccttg
     1741 cttaagaaaa tgtcgctaag acgcggcgcg atctttggcg ggctgatctt gtgcgcactc
     1801 ctcgcttttg aagtgttcaa ctttggcacc acttcatttg ccttgcgcga tgttctcggt
     1861 gatctacaat ttgcggggat gtattggtct tctgttttgg cgtttgcctt ttgcgccatc
     1921 gacttcgccg gtatcgctcg tatctttacc cccgaacagg gtcgcgatga acccgccgaa
     1981 gtttggtatc tctttggcgc gtggctgctc gcggctgctt tcaatgccat gctcacctgg
     2041 tggggtgttt cggttgccat tcgaaaccat aacgccttgg gcggtggttt ggtcggtcaa
     2101 gaaaccatga tgaccgttgt ccccatcttc gttgcctgca tggtttggct catccgcgtg
     2161 ctgttgatcg gaaccttctc tgttgctgga gaacgcctct tttcaatggc ggatatttat
     2221 gaccgcaaca cggcataccg caacgttcct gcttcctact ccacgcctca accccggacc
     2281 gttcagcctg ctccacgcca ggaagtgaat ccacccgtaa acaattaccc gcgacccgtt
     2341 ccgaagccgc agccccgtcc aaacccaatg cctgcttatg gcgccaatcg ccccgaacca
     2401 acctatcatc ctgtgggcat gtctgccatg agccgcaacg agaacagcaa tccctctgta
     2461 cggagatagc ccttgtctgt gagataaggt ttctcgaaat cctcttcccc atccaaccgc
     2521 tgagacgtcc gaaagggcgt ctcatgcgtt aagaacaatt ttattcaaac tcatccaacg
     2581 tactatcacc atgcgttaga acatacatcc cgcgcggact gccttcattg gcatcaaaga
     2641 caaaatcagt aacgatgtaa tttttggcaa atgcagtttc aaacagttca cggctgaaaa
     2701 agcgccaatc tcttgccaga ggaaagtctt tttctttgat ctgcataaaa ttggtgggaa
     2761 tctccaccaa caagagccga tcctcaaagg gtggcacatg ctcgggtgca agcatcaagc
     2821 ctgcgtcgga gaattgaggc gtgtaaaagg ggcggagtcc actgcgggca acatgagcca
     2881 gtttgagagt cgggcgtgga cgtttgctga gtcgcttttc cacacggcgc gaattgatcc
     2941 accaatccac ttgaaaacgg tcggaaggca gcccggcgtt tagtccatca cgcaactcac
     3001 catattcaga ttcacgatag gtgctgcaca ctgcgccaag cttggaaata ttgaggtgtg
     3061 cgttccggct caacaacggg tcgaaggtcc atgtgatgtg gtcgagaccc tgatgccgaa
     3121 ccatttgcca ttgagcacgt ttcagttcaa agccaatgcc actatcgcga tatttcggat
     3181 gaatgcccat ttgatgcgaa caatgcttag ggcgcggacc atctgccgtg cgttcgagcc
     3241 caggaaaacc aaagacaaag ccaatgagtt gttcttcttc aaacgcgccc attaccaacc
     3301 cgccattatg gacaacggtg atcaacacat ggcttgggac gacatctgtc tcagagccgg
     3361 gccaaacctg ccgttgcagg tcttcaacca atttcatttc atcgggagtt tcaagcaggc
     3421 gaatattggg aagggacatg ttatcttctc tgtggggata atcgttcggt gaccgcgctc
     3481 cataagcgtt ggtaccaacg tttgtagatc aagtaatcaa tacggtacga gaaacgagca
     3541 ggcatcaaac caaagtgacg cggcatcgca tcaactgccg tggtgcgatt gacggcgaaa
     3601 taatcaagaa agagcgaaga aagtggaaag cccggcacca cactttcgag aaacaccgtc
     3661 atggcacgca aggtgatcgg cgaaagtggt acaagaaaac ggcgtcgcct tgtaacatcc
     3721 atgataattt caatgatctg ttgcagggta aaaaattcgt tgccacctac ttcgtatacc
     3781 tgattgaccg tactttcatt ttgcaacgcc cagagcatac aagtgacaag gtcttctacc
     3841 catacgggct gcaccaccac tcgtccgcca ttggggacgg ggaaaactcc cggtgaattc
     3901 cgaagcaagc gcgctagatt attggtgaag tgatcttcgg gtccataaac caatgaagta
     3961 cggatgacgg tatatggcac tttgcccgcg cgaatggatt cttcggcaat ccctttggct
     4021 ttgaaggcag ggtaccccga tgcacgcgcc gcccccagat gactaagata gacaatacgg
     4081 tccacgccgg catcggcagc ggcttcaacg aggttttgtg tcccttgaat atcggtgatg
     4141 agcaaacttc cgcgagcccc ttggttttca gcgctggcta agtgaaagac cgtttccacg
     4201 tcgcgcatgg cagcacgcag accgcgcgta tctgcaagcg aaaccaccgc cacctcaaca
     4261 ggcacgcctt tgggcaggcg tggcgtcttg gcagaaggac gaataagagc acggaggggg
     4321 tatccgatag atgacagttg gcgtacgagg gcgcgtccaa tgaatccggt ggcgccagtg
     4381 atgagaatca tggcttggat tatacaggca gattacatcc cgcgaaaaag tctacaaaac
     4441 aaaaacaggc actcgagcga atgcctgttt tctgaatctt ctgaaaaaat ttacgcagta
     4501 ggaggagtct gatcgttgct gacgggagcc gcagcagctt caggcttctt gcgcatggtg
     4561 aagaaaccaa cgcccacagg aatcaggata aagatcaagg cgacacagac taatgcaatg
     4621 ccaacggcag ggggcaagga agattcgtta aaagttccat tgcctgtggc ggtgatggcg
     4681 ccgaacaaac acataaacaa accggggcat ccgcacaaca taacggcggc gatggttgcg
     4741 atcaaaccag tatttttgtt attcattttt tctcctataa attgatttaa ctcagtatat
     4801 gcctaataaa aatgggatgc aagcaaatgc ctgcatccca ttggtactag ttgatctaac
     4861 ttcgcaactt tgcgccgact tctttttcca gccgtttgac gatcttgttg cggatggcgg
     4921 cggcat
//
LOCUS       DAOREF010000086         4923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9675_length_4923_cov_5.367707, whole genome shotgun sequence.
ACCESSION   DAOREF010000086 DAOREF010000000
VERSION     DAOREF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9675_length_4923_cov_5.367707"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PKI33_02735"
     CDS             <1..1690
                     /locus_tag="PKI33_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM35945.1"
                     /translation="LGLRVVTGSLKGLAAEANRIAQGNLDHPLQVTGEDEVAQLRRAF
                     EQMRIGLNARLEEINRLLQVSQGVASSLEMQDAVKPVLEAIISTGANSVRVVLSPSIL
                     PDTFIELPSTFTSGPAEDKYAHLDDQILDLAQSQEKIVIPNLTRSRELMLEPNQPQPL
                     ALLAVALKHENRYYGVVWAGYEQPRKFSDSDVRFMTTLAGQAALAVANAHLYLNVEAS
                     RRQLEAIINSSPDPVIVTDHRNRLLLANHAATSALKQDMDTTRSGMETEKVIKLRPLL
                     ALLQSTTSEKQSTEIVLPDSRTYLATASPVMVEGRQIGRVCIMRDVTHFKELDTMKSE
                     FVATVSHDLRSPLTLMRGYATMLDTVGELNEQQQGYVKKIVTGVENMSRLVNNLLDLG
                     RIEFGVGLQVENVSVLDIIERVTGALQLQALQKNIQLNVELPKDMPHAVEADQALLHQ
                     AVYNLVENAIKYTPPSGRVTIRTVSQPDQLVFAIDDSGIGIPAEDVPHLFEKFYRGKQ
                     REARSQPGSGLGLAIVLSIAESHRGRVWVESTVGKGSTFYLQIPLVQPKETRSV"
     gene            1861..3843
                     /locus_tag="PKI33_02740"
     CDS             1861..3843
                     /locus_tag="PKI33_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNM35946.1"
                     /translation="MIDPEKKQTPIIERKTEYKPPSNLQSWLIGRPLSTADAPHQTIG
                     KRVGLAVFASDALSSTAYATQEILVILAAAGTMAFGYVFPISIAIVVLLAIVSISYEQ
                     TIHAYPDGGGAYIVSRDNLGEFPALIAAAALLTDYILTVSVSIASGVAQLVSAFPSLY
                     NLRVPLAVLFILFIMLINLRGVKESGAAFAVPTYFFIVVILITIATGLWRLITGSLGM
                     VLNPPEIEFHGDAIATITPFLLLHAFSSGTAALTGIEAISNGITAFKEPRSKNAGVTL
                     VWMSIILSTFFLGISFITNYVHAVPSETETVISQLARTIYDGRGTMYLMVISATTVIL
                     IMAANTAFADFPRLGALIAKDGFIPRQLAYRGSRLVYSRGIVTLAVIASILMVLFQAS
                     VTRLIPLYAIGVFLSFTLSQGGMALRWWKSGRLKPGEQKVERGSTIHYEKNWVLKMFV
                     NGFGAVCTAVVMMVFAITKFKDGAYLVLVLIPMLVGVFWMIHRHYNNLAKNLSLDNFG
                     VIPPQTHRHRVIMPVSGVHQGTLAALRYARMLSDDVTAVHIVIEPDDAEKARKKWETW
                     GEGVRLVMLDSPYRLFVEPLLEYIADLAAKRQPGEIITVVVPEFVSENRWTAALHTNT
                     ADILRNQLKNQHGIVITNVPYHVHEAEGDHADHVAH"
     gene            3856..4521
                     /locus_tag="PKI33_02745"
     CDS             3856..4521
                     /locus_tag="PKI33_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNM35947.1"
                     /translation="MNFIVIGCGRFGAELAYKLFQNGHQVVVVDANPKAFNRLHPDFR
                     GRTVEGDSLSADTLSRASADKATGIAVVTNSDTMNAVIGHTIRVHYPNVEQVIVRNYD
                     PAMRDMLEAFGLQIVSSTAWGAERVQELLIDPSFRAVFSAGNGEVEMYEMYISPKWDG
                     MTISNLLDGCGEIVCSALTRAGRAQLPNGGMKLQKGDILTVSATLEGVKALRAKLQEG
                     KEA"
     gene            4523..>4923
                     /locus_tag="PKI33_02750"
     CDS             4523..>4923
                     /locus_tag="PKI33_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNM35948.1"
                     /translation="MLVLIAGGGRTGAQLAAQLLQQNYEVRLVEHRKELLARLHHELP
                     TEVIYEGQATDPNVLRQAGLEKANVIVACTNDDAANLVLCYLARTLFKVRRTVARINN
                     PRNSWLFDNNFHVDETINQADVMAHLIQEEMS"
BASE COUNT         1126 a         1225 c         1281 g         1291 t
ORIGIN      
        1 cctcggtttg cgcgtagtga ctgggtcatt gaaaggcttg gcggctgaag caaatcgcat
       61 cgcgcaaggc aatctcgacc atccgttaca agtgactggt gaagatgaag tggctcagtt
      121 gcgccgcgca tttgagcaga tgcgcattgg gttgaacgcc cgtttggaag agatcaaccg
      181 tctcttgcaa gtgagtcagg gcgtggcatc tagcttggag atgcaggatg cggtcaagcc
      241 ggtgttggag gcgatcattt ccacgggcgc gaactcggtg cgtgtggtgt tgtcgccaag
      301 catcctcccg gatactttta ttgaacttcc atccacgttt acttctggac ctgccgaaga
      361 taagtatgcg catctggatg accagatttt ggatctggca caaagccagg agaagattgt
      421 cattccaaat ctaactcgtt cacgcgagtt gatgctcgaa cccaatcagc cacaaccttt
      481 ggcgttgctg gctgtggcgt tgaagcatga gaatcgttat tacggcgtag tgtgggcagg
      541 ctatgaacag ccgcgcaaat tctcagactc ggatgtgcgc tttatgacga ccttggcagg
      601 gcaggcagcc ttggctgtgg caaatgcgca cttgtatttg aacgtagaag cttcacgccg
      661 acagttggaa gccatcatca attcttcgcc agatcccgtg atcgtgacgg atcatcgcaa
      721 tcgtttgttg cttgctaacc acgcagctac ttctgcactc aagcaggata tggacacaac
      781 tcgcagtggc atggagactg agaaggttat caaactccgc ccgttgcttg cgctattgca
      841 gagcactacg tctgaaaaac aatcgacgga aattgttctg ccggatagcc gcacgtattt
      901 ggctaccgct tcccccgtca tggtggaggg acgtcagatc gggcgtgtgt gtatcatgcg
      961 cgatgtgact cacttcaaag aattggatac gatgaaatcg gagtttgtgg caaccgtaag
     1021 ccatgacctc cgctcgccgc tgacgctcat gcgcggttat gccaccatgc tcgatacggt
     1081 gggtgagttg aatgaacagc aacaaggcta tgtcaaaaag attgtcactg gcgtagagaa
     1141 tatgtcgcgc ttggtgaata acttgctgga tttgggacgc atcgaatttg gcgttggctt
     1201 gcaagtagag aacgtctcgg tgttggatat tatcgagcgc gtgaccgggg ctttgcaatt
     1261 gcaagcgctt cagaagaata ttcaacttaa tgtggaactt ccaaaagata tgccacacgc
     1321 tgtggaagcg gatcaggcac ttttgcacca agcagtttac aacttggtgg aaaacgccat
     1381 taagtacact ccgccttccg gtcgggtgac gatccgaacc gtgtctcagc ctgaccaact
     1441 cgtttttgca attgatgact ctggcatcgg tattccggct gaagatgtcc cgcacttgtt
     1501 tgagaagttt tatcgcggta agcaacgtga agcgcgctct caacccggct cgggcttggg
     1561 tctcgccatt gtgttatcca tcgcagaaag ccaccgtgga cgtgtatggg tggaaagcac
     1621 ggttggcaag ggcagtacct tctatctaca gatccctctc gtgcaaccca aagaaaccag
     1681 aagcgtctag cacgcgtata tccccctgtt gtcaaaataa ggctgacggt atgttgcagg
     1741 acgtatagac tagtagtttt gtatgaccct tgtaggtgat gtctgaaaaa cagaatccga
     1801 ctataatcca gccttgtcct tccgcgggaa gggcatcatt tttgtctgta tgcaggtcct
     1861 atgatagatc cagaaaaaaa acaaaccccg attattgaac gtaaaacaga atacaaacca
     1921 ccaagcaatt tgcaatcctg gctgatcggt cgcccgcttt ctacagcgga tgctccgcat
     1981 cagaccatcg ggaaacgagt cggtctggcg gtcttcgcct ctgacgcgct ttcctctacg
     2041 gcatatgcca ctcaagaaat cctcgtcatt ttagcggcgg cgggcaccat ggcattcggg
     2101 tacgtattcc cgatatccat cgccattgtt gtgttgttgg cgatcgtatc catttcttac
     2161 gaacagacta tccacgctta ccccgatggc ggcggtgctt acatcgtttc gcgcgataac
     2221 ttgggtgaat tcccagcatt gatcgcagcc gcagcgctgt tgacagacta catcctcaca
     2281 gtgtctgtgt ccatcgcttc gggtgtggct caattggttt ctgctttccc aagcttatac
     2341 aacctacgtg tgccgttggc ggtgctcttt atccttttca tcatgctgat caacttgcgc
     2401 ggtgtaaagg aatcaggcgc agcgtttgcc gtccctactt atttctttat cgttgtcatc
     2461 cttatcacca ttgctacagg tttatggcgt cttattaccg gttcactggg tatggttctt
     2521 aacccaccag agatcgagtt ccatggagat gccattgcta ccattacgcc gtttctgttg
     2581 ctgcatgcct tctccagcgg taccgctgcg ttgaccggta ttgaagccat ctcgaacggc
     2641 atcactgctt tcaaggaacc acgcagtaaa aatgccggtg tgacgttggt ttggatgtcc
     2701 atcatcctca gcaccttctt ccttggcatt tccttcatta cgaactatgt gcatgccgtt
     2761 ccttctgaaa cagaaacggt tatttcacag cttgctcgca ccatttacga tgggcgtgga
     2821 accatgtacc tgatggtgat ctctgcgacc acggtcattt tgatcatggc ggcaaataca
     2881 gccttcgcag attttccacg tttgggtgcc ttaatcgcga aagatggttt tattccgcgc
     2941 cagcttgctt accgtggcag ccgtttggtt tattcccgcg gtatcgtgac tctggctgtg
     3001 atcgcttcga tcttgatggt gttgttccaa gcaagtgtga cccgtcttat tcctctatat
     3061 gccattggcg tgttcctttc attcaccctt tctcaaggtg gtatggcatt gcgctggtgg
     3121 aagagtggac ggctcaagcc cggagaacaa aaggtagagc gtggctcaac cattcactac
     3181 gagaagaatt gggtgctcaa gatgttcgtc aatggctttg gtgctgtttg taccgcggtc
     3241 gtgatgatgg tctttgcaat cacaaaattc aaagatggcg cctatctggt tttagttttg
     3301 atccccatgc ttgtgggtgt gttctggatg attcatcgcc attacaacaa cctcgcgaaa
     3361 aatctttcgt tagataactt tggcgtcatc ccaccgcaga cccatcgtca ccgcgtgatt
     3421 atgccggtga gcggcgtcca tcagggaact ctcgcggctt tgcgctatgc gcgcatgctt
     3481 tcagatgatg tgacggctgt gcatatcgtg atcgagccag acgacgctga aaaggctcgc
     3541 aagaaatggg aaacctgggg cgaaggcgtc cgcttggtga tgttggattc gccctatcgt
     3601 ctgttcgtag agccgttgct tgaatacatt gcggatctcg cagcgaaacg tcaacccgga
     3661 gagatcatca cagtggtggt gcccgagttc gtttccgaga atcgttggac ggctgccctg
     3721 cacacgaata cagcagacat tctgcgtaat caattaaaga atcaacatgg cattgtgatt
     3781 accaatgtgc cttatcatgt tcatgaggca gagggcgacc atgccgatca tgttgcccac
     3841 taaggagttt ttactatgaa tttcattgtt attggatgtg gaagatttgg cgctgaactg
     3901 gcgtataaac ttttccaaaa tggacatcaa gttgtggtgg tggatgccaa ccccaaggcg
     3961 ttcaatcgcc tccacccaga ctttcgcggg cgtaccgtgg aaggtgactc tctctccgct
     4021 gacaccttat cccgcgccag cgccgataag gcgaccggca tcgctgtggt caccaattca
     4081 gacacaatga acgccgttat cggacatacc attcgggtgc attacccgaa tgttgaacaa
     4141 gtcatcgtcc gcaattatga ccccgccatg cgcgatatgc tcgaagcctt tggactgcaa
     4201 atcgtcagct ccacagcatg gggtgcggag cgtgttcagg aactcttgat tgacccatct
     4261 ttccgtgcgg tattctctgc cgggaatggc gaagtagaaa tgtatgagat gtacatctcc
     4321 ccgaaatggg acggcatgac catctccaac ttgctggatg gttgtggcga gattgtctgc
     4381 tctgcattga ctcgcgctgg gcgtgctcaa ttgccgaatg gcggaatgaa attacagaag
     4441 ggtgatattc tcaccgtaag cgcgaccttg gaaggcgtga aagctttacg tgcaaaattg
     4501 caggaaggca aggaggcata acatgcttgt acttattgct ggcggcgggc gcactggcgc
     4561 tcaactggct gctcaacttc tccaacaaaa ttacgaggtg cgcctcgtag aacatcgcaa
     4621 ggaattgctg gctcgcttgc atcatgaact tcctaccgaa gttatctatg aaggtcaggc
     4681 gacagacccg aacgtactca gacaagctgg tttggaaaaa gccaatgtga tcgtggcttg
     4741 taccaacgat gatgctgcaa atttggtttt atgctatttg gcgcgtaccc ttttcaaggt
     4801 tcgtcgcacg gtggctcgta tcaataatcc gcgcaatagc tggctgtttg ataacaactt
     4861 ccacgtggat gaaaccatca accaagcgga cgttatggct cacctcattc aagaagaaat
     4921 gtc
//
LOCUS       DAOREF010000087         4903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9733_length_4903_cov_3.847772, whole genome shotgun sequence.
ACCESSION   DAOREF010000087 DAOREF010000000
VERSION     DAOREF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9733_length_4903_cov_3.847772"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKI33_02755"
     CDS             complement(<1..354)
                     /locus_tag="PKI33_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM35949.1"
                     /translation="MQTLNQQFELIRQSATVSLTDRILALKASGKPIISLQVGDPDFG
                     THPAIAEAALKAMQEGQTHYGPSNGTLELRKAIAHKLQRDEGVSYDPNTEILVTNGGI
                     HAYYTAMQSILNPGDD"
     gene            complement(424..1689)
                     /locus_tag="PKI33_02760"
     CDS             complement(424..1689)
                     /locus_tag="PKI33_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM35950.1"
                     /translation="MTDSQTKILSRLLEVTRSLDSEADLESSLRLILSAATELTASET
                     ASLLEYDETALNFFFKYVPWFNQDSQGGSTLRIPLNESIAGWVLLNKSSVTVNDAIND
                     PRHSKKVDEFAGITIHSVLGAPLIVRDVPIGVLEVFNKRSNYTEEDVLIVENLAALAA
                     AAMEKDLLEKKVASSKEEARELDRMKNEFIAITSHELRTPLGLILGHSTFLKELLGGS
                     YEDQVDVIIRNASRLKEIIESLTSVDNYQSGGALVRSRKVSIARIIEDVIVSFSEMAN
                     KKKITIKKALQPGYELWVDVDAGKIAIVLSNIMKNAITFTNEGGEIIIRGEQHPDYVK
                     VSVKDNGIGIPTKDLPHVFDRFYQVEGHLTRRHGGMGLGLSVAKVMVEMHGGRIWADS
                     EEGKGSIFSFILPVRSEPPAPASSSPFSI"
     gene            1789..2877
                     /locus_tag="PKI33_02765"
     CDS             1789..2877
                     /locus_tag="PKI33_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNM35951.1"
                     /translation="MANKKKVRVAIIGVGNCASSLVQGVQFYKNAKNDDVIPGIMHPQ
                     LGEYHIGDIEFTLAIDINATKVGKDLSEAIFAEPNNTYKFCDVPHLNVPVVRGMTHDG
                     LGKYLSQVIEKAPGSTADIVRLLKETKTEVVISFLPVGSEMATKWYVEQIIEAGCAFV
                     NGIPVFIASSEYWGKRFADAGLPILGDDIKSQVGATILHRQLATLFVDRGVKIERTYQ
                     LNFGGNTDFLNMLERERLESKKISKTNAVTSMIPYEINPDNVHVGPSDHVPWLTDRKW
                     AYIRMEGTTFGNVPLNMEVKLEVWDSPNSAGVMIDALRCAKIALDRELAGPIVGPSSY
                     FFKTPPKQFRDEIAKQKVEDFVSGKSDE"
     gene            2958..4136
                     /locus_tag="PKI33_02770"
     CDS             2958..4136
                     /locus_tag="PKI33_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35952.1"
                     /translation="MRRKTTRHSLIVSRLFLSLSLFATLLAACQPAAPTTDINAALTA
                     AFETAVAQINAPTATPQITDTPIPTATVPRTPPALPGTYQSGILKPNDIPRTYVADSC
                     QYLKDKWDSTKSAPGTVVMTIMSHRINQDAATSDPASLTISSKDLKRLMNSLHDAGFT
                     AINMQQMSDFMYNNAKIPQLSVLIIVDDRSSSSKYNDHFRKYYEEWGWPVVNAYIGLD
                     ERPDLWAENAVLSSEGWVDYEAHGYIHNIPISDGSTDDFITSEMGGVITTFQKYFNKT
                     PIAYIWPGGGFSKRAAEIGVQLGYKLGFTVNPRGPVMYNWIPQADAYNEDNPYAIPET
                     PAGNPLMTLPRYMDITAGDHIDEVRVMSQEAAAYAEQNKATELEYYDIVCAPTYGPIP
                     "
     gene            4240..>4903
                     /locus_tag="PKI33_02775"
     CDS             4240..>4903
                     /locus_tag="PKI33_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35953.1"
                     /translation="MQSPRNTFIPIFLRLTSIVSLVSILVSCQLSPSITNTAEAPTEI
                     ADQTIPVLPETFQTDLLNPVDVPHTYVEETCRYLRNKWNPANAQPGTVVMLILIKDIN
                     RGAVELPNSIRITDFYDLLEQLKAQGFEAINTEKLQAFLERNLPIPPRSVVLIQDGNH
                     TEEYFDKNYRWYYEQWGWPVVNGFVSDPNIDPILREGNFSLEYEGFIDHQARGVTPDV
                     VLT"
BASE COUNT         1360 a         1259 c         1031 g         1253 t
ORIGIN      
        1 gtcatcaccc ggattcaaaa tggattgcat cgccgtatag tatgcatgaa tgcctccatt
       61 tgtaacgagg atttctgtgt tggggtcgta gctcacgcct tcatcgcgtt gaagtttgtg
      121 tgcaatcgct ttgcgaagtt caagtgtgcc attggaaggt ccataatgag tttgaccttc
      181 ttgcatggct ttaagtgccg cctcggcaat ggcaggatgt gttccgaaat cggggtcacc
      241 gacttgcaag ctaatgatgg gtttgccact ggctttgagc gcaaggatgc ggtctgtcaa
      301 cgaaaccgtg gcagattggc gaatcaattc aaattgctga ttaagggttt gcataagatg
      361 tccttcaaag aatgggcgtg agtgtatagg gaaataaaaa tcccgtcaac ggtgtgacgg
      421 gactcaaatg gaaaagggag aagaggatgc tggcgctggc ggctcgctgc gaacaggaag
      481 aataaaggaa aagatactcc ctttgccttc ttcgctatca gcccaaatac gcccgccatg
      541 catttcaacc atcaccttgg caacggaaag tcccaacccc atgccaccgt gtcggcgtgt
      601 gaggtgacct tccacctgat agaagcggtc aaacacgtgt ggcaagtctt tggtaggaat
      661 cccaattccg ttatctttca ccgagacttt tacataatcc ggatgctgtt caccacggat
      721 gatgatttct ccgccttcat tcgtaaatgt aatagcgttc ttcataatat tgctcagaac
      781 aatggcaatt ttcccagcat ctacatccac ccacaattca tacccaggct gaagagcttt
      841 tttgatcgtg atctttttct tattcgccat ttcagaaaaa gagacgatca catcttcaat
      901 aattcttgca atagaaactt tgcgcgagcg gaccaatgcc ccaccgctct ggtagttatc
      961 cacgctggtt aagctctcga taatttcttt caaacgagag gcattacgaa tgatgacatc
     1021 cacctggtct tcataactcc cgcctaataa ttctttcaag aacgtagaat gtccaaggat
     1081 gagtcccaag ggtgtgcgca gttcatgtga tgtgatcgcg ataaactcat tcttcatccg
     1141 atcaagttca cgcgcctctt ctttcgacga agcgactttc ttctcaagaa ggtccttttc
     1201 catcgctgca gccgccagtg ccgccaagtt ttcaacgatt agaacatctt cttctgtgta
     1261 gttactgcgt ttattgaaga cttccaaaac cccaatcggc acatcgcgaa caatcaacgg
     1321 tgcccctaaa acggagtgga tggttatgcc tgcaaattca tccacctttt tggaatgacg
     1381 cgggtcattg atcgcatcgt tcacagtcac cgatgattta ttcagcaaaa cccaaccggc
     1441 aatactctca ttcaacggaa tacggagcgt acttccacct tgtgaatctt gattgaacca
     1501 agggacatat ttgaaaaaga aatttagagc cgtctcatca tattccaaaa gagacgcggt
     1561 ttcgctcgcc gtcagttcgg tagcggcaga gaggattaaa cgtaaactgg actccaaatc
     1621 cgcttcagag tcgaggctgc gtgtcacttc gaggagacgt gaaaggattt tcgtctgtga
     1681 gtccgtcata caaataacct tgggtaagat tatacttcac ggttgtatct gaaaatcgac
     1741 cctcataatt caataaggaa actgtaatcc acctcaggag ctaaacaaat ggcaaataag
     1801 aaaaaagtac gtgtggcgat cattggcgta ggaaactgtg cctcttccct cgtgcaaggc
     1861 gttcaattct ataaaaatgc caaaaatgac gatgtgattc ctggcattat gcacccgcaa
     1921 ttgggtgaat atcacattgg cgacattgaa ttcaccctgg cgatcgacat caatgccacc
     1981 aaggttggca aagatttgtc tgaagcaatt tttgcggagc cgaacaatac ctacaagttc
     2041 tgtgacgtgc ctcacctcaa tgtacccgtc gtgcgcggca tgacccatga tgggttgggc
     2101 aagtatctca gccaggtcat tgagaaagcg cccggctcca ctgccgatat tgtacgccta
     2161 ttaaaagaaa caaagaccga agttgtcatc agcttcctcc cggtaggctc agaaatggca
     2221 accaaatggt atgttgagca gattattgaa gcaggttgcg cctttgttaa cggtattccg
     2281 gtattcattg cctcctctga atattggggc aaacgcttcg ccgatgccgg tttgccaatt
     2341 ctcggtgacg atatcaaaag tcaggtcggc gctactattc ttcaccgcca gctcgccacc
     2401 ctgttcgtag accgaggcgt caagatcgag cgcacctatc agctcaactt tggcggcaat
     2461 acagacttcc tcaacatgct cgaacgcgaa cgccttgaat ccaagaaaat ttccaagacc
     2521 aacgccgtca ccagcatgat cccatacgaa ataaatccag ataatgttca cgtcggtccc
     2581 agcgatcacg tcccctggtt aacggatcgc aaatgggcat acattcgcat ggaaggtacc
     2641 accttcggca acgttccgct caacatggaa gtgaaacttg aagtgtggga tagccccaac
     2701 tctgccggcg ttatgatcga tgctctacgc tgtgccaaga tcgcgctcga ccgcgaactt
     2761 gccggtccga ttgttggacc atcctcttac ttcttcaaga ctccgccaaa gcaattccgc
     2821 gatgaaatcg caaaacaaaa agtggaagat ttcgtctctg gcaaaagtga tgaataacaa
     2881 ataacagaaa aggcgcacag aaatctggtg cgccttttct gttattattg acagtcacaa
     2941 aaggatataa attcatcatg cgacgcaaaa ccactcgtca ctcgttaatc gtttctcgcc
     3001 tctttcttag ccttagcctg tttgccacac ttctagccgc ctgccaaccc gctgccccca
     3061 ccaccgacat caacgccgca ctgacagccg cttttgaaac cgccgtggca cagatcaatg
     3121 caccaactgc aacaccccaa attactgata ctcccattcc aactgcaacc gttccacgta
     3181 cgccacctgc tttgccaggc acatatcaat ccggaatcct aaaaccaaat gacatccctc
     3241 gcacctatgt cgcggactca tgccaatacc taaaagacaa atgggactcg accaaatctg
     3301 cgccagggac cgtagtcatg accattatgt ctcaccgcat caatcaggat gcggctacct
     3361 ctgaccctgc aagtttgact atttcaagca aagacttaaa acgtttgatg aactccttac
     3421 atgatgctgg ctttaccgct atcaatatgc aacaaatgtc agatttcatg tacaacaacg
     3481 caaaaatccc tcaactttcg gtactcatca ttgtggatga tcgttcaagt tcttcaaaat
     3541 acaatgacca ctttaggaaa tattatgaag agtggggctg gcccgtggtc aatgcatata
     3601 tcgggttaga cgaacgcccc gacctttggg cagaaaatgc cgtactctcc tctgaaggct
     3661 gggtagacta tgaagcccat ggctacatcc ataacatccc cattagtgat ggttctacag
     3721 atgacttcat cacctcagaa atgggtggtg tgatcactac tttccaaaaa tatttcaaca
     3781 agacccccat tgcctatatc tggcccggtg gtgggttttc aaaacgagcc gctgagattg
     3841 gcgttcaact gggatataag ttaggcttta ccgtcaaccc acgtggaccc gtcatgtaca
     3901 actggattcc tcaagcagat gcctacaatg aagataatcc atatgccatt cctgaaactc
     3961 cagccgggaa tcctttaatg accctcccgc gctatatgga tatcaccgca ggagatcaca
     4021 ttgacgaagt tcgtgtcatg agtcaagaag cggctgccta tgccgagcaa aacaaagcca
     4081 ccgaattgga atactacgac attgtttgtg ctcccacata cggacctatc ccctagttaa
     4141 gaattctttc taagtacaac ctaaacaggt caggcaatgc ctgacctgtt tatataagca
     4201 aaaacttatt ctcaaaaact ggtttacaat tctatttata tgcaatcgcc aagaaatacg
     4261 tttattccca tctttcttcg cctcacatcc attgtctcac tcgtttccat tctggtttcc
     4321 tgccagttaa gtccatctat cacaaataca gcagaagctc ctactgaaat agcagatcaa
     4381 accatccctg ttttaccaga aacgtttcaa accgatttat taaaccccgt tgatgttcca
     4441 catacctatg tagaagagac ctgtcgttat ttacgcaaca agtggaaccc tgcaaatgcg
     4501 caaccgggca ccgttgtcat gctcatcctt atcaaggata taaatcgtgg agccgtggaa
     4561 cttccaaata gtattcgcat cacagatttc tatgacctat tagagcaatt aaaagctcag
     4621 ggtttcgaag cgatcaatac agaaaaacta caagcatttc tggaacggaa ccttcccatc
     4681 cctccgcggt ctgttgtatt aattcaagat gggaatcaca cagaagaata tttcgataaa
     4741 aactatcgct ggtattatga acagtggggc tggcccgtcg tcaatggatt cgtcagtgac
     4801 cctaatatag accctatctt acgcgaaggg aatttctcgc ttgaatatga aggcttcata
     4861 gaccaccagg cgcgcggcgt cacacccgat gtagttctca ctg
//
LOCUS       DAOREF010000088         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9830_length_4877_cov_3.002074, whole genome shotgun sequence.
ACCESSION   DAOREF010000088 DAOREF010000000
VERSION     DAOREF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_4877_cov_3.002074"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..630)
                     /locus_tag="PKI33_02780"
     CDS             complement(421..630)
                     /locus_tag="PKI33_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNM35954.1"
                     /translation="MIKIKVPELLKKKGLNATDLMRKGNIAYGTAHRLSKGDGDGITF
                     EVLNALCKLFNVQVKDILEYVPDDE"
     gene            complement(995..2485)
                     /locus_tag="PKI33_02785"
     CDS             complement(995..2485)
                     /locus_tag="PKI33_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35955.1"
                     /translation="MSQLSIKNWGTQFEKAILQGYESRKAFVHEINEYFRKKHEGHLK
                     ILEDFKQAQEQKKTISSEIYENDLVKKYRSGQKISIPSQWIWDTEITKWVNGKKSPTN
                     SKTLIPILEFFFEKNIMSKIEANEWIMNLKFVMPDKELHKIIKYIGANTSSFTANPWI
                     LDKNKIQEFMYIGTLFVVYGPVGCGKSNFIEHVTNYLHHHFRSEISKGVFLVSGKNMS
                     VGNALFHIMSNVLECLPTNENEIFEIWDDWITTYIDKDIVVILDNVSPELVSYFIKYP
                     TKIHLIVSTSDIENILPIISDAIAHNEVIFQTPTINKASLINYLSEYTRLHRDWVMNI
                     AMLSEWYPEPIFRAIKLISGKNKIDWFGRLGDLPVKTTSVFNAHWSYPEYATGLHKVA
                     RSILASLREKERHYMLLFVLFAKSDEFYSIEVISKVWKLENALALFVIERLEKLGLLI
                     RQIEENPIHGYVFNYRPLRFFIEAVQIANNFNQDPEIFLLSSEELF"
     gene            2825..2992
                     /locus_tag="PKI33_02790"
     CDS             2825..2992
                     /locus_tag="PKI33_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35956.1"
                     /translation="MSTTTKKPSEQKRIQDFTRELGRILRTIADTVGKPKPEKKPVQK
                     TTKKEQPANDQ"
     gene            2982..4790
                     /locus_tag="PKI33_02795"
     CDS             2982..4790
                     /locus_tag="PKI33_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNM35957.1"
                     /translation="MTNNFPFERGDILSLISEEVAKSRPDNVWAIYSRKSRVKPNDPG
                     YSMEYQPETAEKYARDHGAKEVLFFQDVDRSGKNSKREGFQDLLRAVKSGRVDVVLVH
                     RIDRIFRNHRSTLELIDLLKKHKVIFISVTENIDTSTWWGRLVLAVLSSLAEAYILQT
                     SDRNREIFDSVRSHGKQLGRHPLGYCNGLCSTCKDFNGNGYCPLYGMPDRPESLRGTL
                     MVPHPMDRHAVVLIYNLYKQGWSFRDIADYLNKSLVALPDGTQVKFRPRGLYGVKRRE
                     GEQKFSRESIRVILSNPDYAGYIPERRRPQLSMEDDLEHPENIPNAKIDGNAREILSL
                     HPANHESLIPYSLWLEVQSLKKTRATAPTAISGKPARKYPLSGVARCWECYETLEKEY
                     TLRGSAGGRGHIYYRCAYTQDIAPSRKKNRKTPRVEGVNTMINVADQQLVARHKQIPA
                     RKLEEQIDSLMAHLVIPREWEDMIAAYFLDDDGMAAVDRAGHTFQRDLQKLKDQYEAG
                     LISGPELERKSRLLQGSLKELMPSSKPESKEILKELRPFGIFWQQLQDGDKHILLGIV
                     FDSLYFNHEGKLIRALANEPFDRLLNLPSGGLMVDE"
BASE COUNT         1443 a          961 c          984 g         1489 t
ORIGIN      
        1 cttttaaaag aagatggctt acaaggtccc tgggtattgg gctcttgcct gcctttcaga
       61 ctagttgtat tgtaaagctc ccaatattag ggcaagtgac cgaatctgtg aataaccata
      121 acaaccaatt tttagactct agtctttttt tctggatcta aaatccacca aaacaagatt
      181 gggggcagta aacataacaa ataaatgtat tcaatagtgg ataatatttt ttcctgtaag
      241 actgaaaaga tcacagctgt aataaaagca aaggttaaca aaatcgtgtc ccgttttgtt
      301 agcccatttg gagttgactt attctgaggg attttcttgg agctttgcag taaaaaacct
      361 agcgtaatta acactgctac caatataact gctccacctg tatctgcgct tggatatggg
      421 ttattcatca tccggcacgt attctagaat atctttgact tgaacattga ataacttgca
      481 gagggcattc agcacttcaa aagtaattcc atctccatca ccttttgata gtcgatgcgc
      541 tgtcccataa gcgatattcc cttttcgcat aagatcagtt gcattcagtc cttttttctt
      601 taacaattca ggtactttta ttttaatcac aagtcacact ccatgttgga atgaccggtg
      661 ataaaagggg cgcaacttga caaagaaaaa aaatctaata caatgtatac agtctgctgc
      721 ataaaagcaa gcatgaccgc cgttgtccaa gcggcgtcca attcccggtc aggcattgtt
      781 gattgtggcc agcagtgcct gacctacatt atagtgtagt tatttcagta taaatagtcc
      841 attgaattaa aaatagagac ctcgccgggt atcctccgtg cgaggttttc gtaaacttat
      901 agagtaagcc acaatgcgcg atccgtaggt ggtcaatagc aacagccagg atgccccctc
      961 atcctggttg ttgctattga gattatttca aagatcagaa taattcttcg gatgaaagaa
     1021 gaaaaatttc tggatcttgg ttgaaattat tcgcaatttg tacagcttca ataaaaaaac
     1081 gaagggggcg ataattaaag acatagccat gtattgggtt ttcttcgatt tgtcttatga
     1141 gtaaacccaa tttttccaat ctttcaataa cgaataaggc tagagcattt tcaagtttcc
     1201 atactttaga aataacttca atgctataaa attcatcaga ttttgcgaat agtacaaata
     1261 atagcatgta gtgtctttct ttttctctta agctggctaa tattgatcgt gcaaccttgt
     1321 gcagtcccgt ggcatattct ggataactcc aatgggcgtt gaacaccgat gttgttttta
     1381 cgggcaagtc tcctaatctc ccaaaccaat caattttatt tttcccgctt attaatttga
     1441 ttgcacgaaa tattggctca ggataccatt ctgacaacat ggcaatgttc ataacccaat
     1501 cccgatgtaa ccttgtatat tcggacaaat aattaattag gctggcttta ttaattgtag
     1561 gcgtttggaa aatcacttca ttatgagcga ttgcgtcact aataatcggc agtatatttt
     1621 cgatatctga cgttgaaacg attaagtgga ttttcgttgg atacttaata aaatagctta
     1681 ctaactcggg cgacacatta tccaaaatta ctactatatc tttgtcaata taggtggtaa
     1741 tccaatcatc ccatatctcg aaaatttcat tttcattagt gggtaagcat tctagaacat
     1801 tggacataat gtggaataat gcgtttccta cgctcatgtt tttgccactt accagaaaaa
     1861 caccctttga tatttcactc ctaaaatgat gatgtaagta atttgtgaca tgttcaataa
     1921 aatttgattt tccacatccc accggtccat acaccacaaa caaagtgcct atatacataa
     1981 actcctgaat tttgtttttg tccaaaatcc atggatttgc tgtaaaagaa cttgtgttcg
     2041 cgccgatgta cttaataatt ttatgaagct ctttgtctgg cattacgaat tttaagttca
     2101 taatccattc attagcttca atttttgaca taatattttt ttcaaaaaaa aactcaagaa
     2161 tggggattag ggttttggag ttggtgggag attttttccc attgacccat tttgtaattt
     2221 ccgtatccca aatccattga ctgggtatgg agattttttg tccagatcta tatttcttga
     2281 ccaagtcatt ttcatagatt tcactagaaa ttgtcttttt ttgttcttga gcctgcttga
     2341 aatcttcaag aatttttaag tgcccctcat gtttttttct gaaatattca tttatctcat
     2401 ggacaaaagc ttttctactt tcgtaacctt gcaatattgc tttttcaaat tgagttcccc
     2461 agttcttaat ggatagttgt gacataggcg aaacattaat ttccttgttg atttttactt
     2521 tctttgaagt tttggttttt ctgaactata tgaatttaat ttatttctca tgaaataatc
     2581 catatagcac attttgtttt caattaattt tctcacattt tcacttttga ttgaaaaaag
     2641 tttattaatc attattgaat gctcattttt aagtattttg actttgatga ttttcacctg
     2701 tgaggtaatc gctttcaatt attgcattct gtgcgattgt gcttttgagc aataaagtaa
     2761 tcgcatattc aattgaatta gctcactagt ggcagatcat aaacttagaa aagagaaaac
     2821 aataatgagc accaccacaa aaaagccctc cgagcagaag cgcattcaag acttcacccg
     2881 tgaattaggg cgtatacttc gcactattgc ggataccgta ggcaaaccca agccggaaaa
     2941 aaagccggtg cagaaaacaa cgaaaaagga gcagcccgca aatgaccaat aatttcccct
     3001 tcgaaagagg cgatatcctc agccttattt cggaagaggt ggcaaaaagt cgtccagata
     3061 atgtctgggc gatctactcg cgaaaatcac gagttaaacc taatgatcct ggatattcaa
     3121 tggaatacca accggaaacg gcggaaaagt acgccagaga tcatggagcc aaggaagtac
     3181 ttttctttca agacgtggac agatctggta agaatagtaa gcgcgagggg tttcaggatc
     3241 tgcttcgagc ggttaagtca ggtcgggtgg atgtcgttct ggtgcatcgt atagaccgca
     3301 tcttccgtaa tcatagatca acattggaat tgatcgacct gttgaagaaa cacaaagtca
     3361 tatttatttc agtaacagag aatattgata cgagtacttg gtggggacga ttggtgctgg
     3421 cagttctttc gagtctggct gaagcctata ttttacaaac cagcgatcgt aacagggaaa
     3481 tctttgattc tgtgcggtca catggcaaac aattaggcag acacccgctt ggatactgta
     3541 acggtttgtg ttcaacttgc aaagatttta atggaaatgg gtattgcccg ctatatggca
     3601 tgcccgatcg tcccgagagt ctacgcggta ctttgatggt tccacaccca atggatcgcc
     3661 acgcggtcgt gttgatctac aatctctata agcagggatg gagctttcgt gacattgcgg
     3721 attatctgaa caagtcattg gtagccttgc cggatggaac tcaagtgaaa tttcgccccc
     3781 gagggcttta tggggtgaag cgtagggaag gagaacagaa attcagccgc gaaagtatcc
     3841 gggtgattct ctctaatcca gactatgctg gctatattcc agaaaggcgt cgtccgcaat
     3901 tgagtatgga agatgatctg gagcatccag aaaatattcc caacgccaaa atagatggaa
     3961 atgcccgcga gatactaagt cttcatccag ctaatcatga atctttgatt ccgtactcct
     4021 tgtggctcga agttcagtcg ctcaaaaaga cacgcgcaac agctccaact gcgatttcag
     4081 gaaagcctgc ccgtaagtat ccacttagtg gtgttgcccg ttgttgggaa tgctatgaaa
     4141 cccttgaaaa agaatacaca ttacgaggtt cggccggtgg gcggggacat atctattatc
     4201 gttgtgcgta cacccaggat atagcgccca gcagaaagaa aaatagaaaa acaccacgag
     4261 ttgaaggtgt gaatacaatg ataaatgtgg cagaccaaca attggtagcc cgtcataagc
     4321 agatccctgc caggaagctt gaagagcaga ttgattcttt gatggctcac ttggtaatcc
     4381 cgcgcgaatg ggaagatatg attgccgctt attttcttga tgacgatggc atggcggcgg
     4441 ttgatcgagc ggggcatacc tttcaaagag atttgcagaa actcaaagac caatatgaag
     4501 cagggcttat ctcgggtccg gaacttgaac ggaaatcgcg actgctacag ggctcgctta
     4561 aagaattgat gccctcatcc aaaccagaaa gtaaagaaat tctcaaagaa cttcgtccgt
     4621 ttggtatatt ttggcagcaa cttcaggatg gtgacaaaca tatcttgctg ggtatcgttt
     4681 ttgacagcct atatttcaat catgaaggca aattaatccg agcactcgca aatgagccgt
     4741 ttgatcgttt acttaacttg ccatcgggtg gtttgatggt ggatgaatag aaaaaaagaa
     4801 caggcttaca cagtctgttc tttttcgtta atcgccaaaa tattttttta gtaaaacccg
     4861 caccgcttcc gcgactt
//
LOCUS       DAOREF010000089         4875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9833_length_4875_cov_4.785892, whole genome shotgun sequence.
ACCESSION   DAOREF010000089 DAOREF010000000
VERSION     DAOREF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9833_length_4875_cov_4.785892"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1894
                     /locus_tag="PKI33_02800"
     CDS             200..1894
                     /locus_tag="PKI33_02800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM35958.1"
                     /translation="MKKVNHLQHETLTGLHGVIIFSLDIYYQYTGFTESHKQTMKLLW
                     GAEIDLGQNILECIQHEDDRKRAQENFDKAIRGELIIVEEEYGDALKHVRLWWENRYS
                     PIYDKDRKIVGVTVFAIDVTQRKYIEHALRTSEIHYRSIMDQASDGIFIADPSGRYVD
                     VNPSGCELLGYTREEILNLTMYDIAVGTKFNPIRIKELMDGKVILTERELLGKNGKLV
                     PVEISAKKMEDGHLMGIIRNIETRRQEEARVRFLAFVMDKISSAVISTDTDMKINYWN
                     KSAEILYGWKEEEVLGQNIDEVCGTEFLEGQQELAKNALLYEKTWRGELKQNHRNGKE
                     IWVDASVTLLEDNSGNYIGGVTINRDVTERKLAEDELRRTKESIEQINLTLRRAFERE
                     QLASRTDSLTGVFNRRYFFELLGYEFLASRRYDRPLSIVMFDVDFLKKINDTYGHQVG
                     DDVLKKAAEVVRTELRSSDVLARYGGDEFAILLSSSSQEEAAMVLERIYLALRSAYVE
                     VGDKKIHVTISAGVSSIRPDMEKADQLVMLADQALYLAKNAGRNRMVIFDESKKIN"
     gene            1978..3132
                     /locus_tag="PKI33_02805"
     CDS             1978..3132
                     /locus_tag="PKI33_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM35959.1"
                     /translation="MNFEPGIDLKTIILVVEDEAAIRLGLSAAMSRQGYRVITAENGN
                     DAIQKAGEYHPDLIISDVMMPVLDGFEMKKKLSADPDLASIPLIYLTARTAVEDRVKG
                     IRDGADDYVTKPFDIDELAARVEAVLRRVRSERKRGHEQAKEFDQDDLEKLRRELMQN
                     FHHEIRTPLNNVMMFLEIVATHKFESLEEQKEFVRIARSSGERLESLISDIVLLTDLD
                     QGLLNCVRQTINPDIHLLQPIRRRISRYDGKVVHFLPILSINDEIKAPRQEFPRAILH
                     LVDNALKFSPEDGTVILKVTSSKNGGATITIEDQGPGIPVGLREKVFERYYQISSGAT
                     RLHQGLGVGLTLARAVLRSLGGDVKILDSAQGCCVEAVLPDLAEDDIAYG"
     gene            3125..>4875
                     /locus_tag="PKI33_02810"
     CDS             3125..>4875
                     /locus_tag="PKI33_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM35960.1"
                     /translation="MDEKVLPLKAIGVDIKDTSWNGDLDYRALFEQTGDGVFIIGLDF
                     NVLAANPPANRMLGYVDGELQGTSAEIFLNLKQGFQKELILEKRVNVFEHNLRKKDGS
                     VLQAELSSSLVYNVEDKPAYIQLIARDIGERKKNERLLKRNVRAQSVIGEVTAALFRS
                     TNIEGSLPEVLESLGYAVGVFSTLIFEVKNSSLRVRHRWVDPTVPEFDAETVLQPFLD
                     SLSKFPDRVFSLPELPEKVASIPKISMLAIPIQGALGTWGFLGLFDKEDRLSWLPTSF
                     DIVQTTANLLGAAIERMHYEETLRLSERRNRIIVDALPDLLLRVDRSGRIFDYNATSS
                     HPLYLEPASAIGKNLYELWNDDIAGKLLPRLPDETLFNLPRTVENFKLPGKLGSFEAR
                     LFPLSKLEALIIVRDITEQARLNQMKSDFINWASHELRTPLTSAILMTELLRQECTPE
                     EAEEYLSTLSSELNRQKNLINELLMAGRLENGTLHVEVAAVDLHPVLKDAILSVKPIA
                     RKRNIAIQMVTAANPCFVMGNVSALQQVFINLLSNAVKFSPDDSQVILRVDRKDTETH
                     VAVVDSGLGIPSDAMPYL"
BASE COUNT         1367 a          871 c         1239 g         1398 t
ORIGIN      
        1 gctataagtg atctttcgag ggaatacaaa ttaaggggta tttgcttgaa tagactcatg
       61 atagataagg taaatggcta ttaattgaga tgtagaatga tgctaggata aaaataatct
      121 cttgtttgta tacgtttagt agtatacaaa caaggccgca gcacccttta gttttaatta
      181 ttgaaagatg atcgttaaga tgaaaaaggt aaaccatctt cagcatgaaa ctcttacagg
      241 cttgcatggc gttattattt tttctcttga tatttattat caatatacgg gatttacaga
      301 gtctcataaa cagaccatga aattgctttg gggggcagaa attgaccttg gacaaaatat
      361 tcttgaatgt attcaacatg aagatgaccg aaaaagggcg caagagaatt tcgataaagc
      421 catacgtggt gagttaatca ttgtagaaga agaatatggg gatgcattga aacatgtccg
      481 tttgtggtgg gagaatcgtt attcgccaat atatgataag gatagaaaaa ttgttggcgt
      541 tactgttttt gcaattgatg ttacacagag aaaatatatt gaacatgcgc tacgcactag
      601 tgagatccat tatcggtcta ttatggatca agcatctgac ggaattttta ttgctgaccc
      661 atcaggtcga tatgtggatg tgaatccttc tgggtgtgaa ctgttggggt atacccgtga
      721 agaaatcctt aatctgacca tgtatgatat tgcagtggga acaaaattca atcctattcg
      781 aataaaagaa ttgatggatg gtaaggtcat tcttaccgag cgggaattac tcggaaaaaa
      841 tgggaaattg gtgcctgttg agattagtgc aaagaaaatg gaagatggac accttatggg
      901 gatcattcga aacattgaga cacgcaggca agaagaggcg cgtgttcgtt ttcttgcctt
      961 tgtaatggat aaaatttcta gtgcagtgat ttcaactgat acagacatga aaatcaatta
     1021 ttggaataaa agcgcagaaa ttttatatgg gtggaaagaa gaagaagtat tgggacaaaa
     1081 tattgacgaa gtgtgtggaa cagagttttt agaagggcaa caagaacttg ccaaaaatgc
     1141 ccttttgtac gaaaagacct ggcgcgggga attaaaacaa aaccatcgca atggcaaaga
     1201 aatatgggtc gatgcttcag taactttatt ggaagataac agtggaaatt acattggcgg
     1261 tgtaactatt aatcgcgatg ttactgaacg taagttggca gaagatgaac tgcggcgtac
     1321 gaaagagtcg attgaacaga ttaatttgac tttgaggcgt gcttttgagc gtgaacaact
     1381 cgcctcacga acagacagcc taacaggtgt gtttaaccgt aggtatttct ttgaattgtt
     1441 ggggtatgaa ttccttgcgt ccaggcgata tgaccgtccg ctttctattg tgatgttcga
     1501 tgttgatttt ctaaagaaaa taaacgacac ctatggtcat caagttgggg atgatgtctt
     1561 gaaaaaggct gcggaagtag ttcgcaccga attgcgttcc tcagatgtgt tggctcgtta
     1621 tggcggtgat gagtttgcga ttcttctatc cagtagtagc caagaagaag ctgccatggt
     1681 ccttgagcgg atttatcttg cattacggtc tgcttatgta gaagtgggag ataaaaagat
     1741 ccatgtgacg ataagtgcgg gggtttccag tattcgccct gatatggaaa aagcagacca
     1801 actggtgatg ttggcagatc aggctttata tcttgctaaa aatgcagggc ggaaccgtat
     1861 ggtaatcttt gatgagagta aaaaaataaa ttaatcttag atatatcagc aaaaatttgt
     1921 catggcttta ttatgctata cctgcccgtt gcggggataa ttcgggttag gtgcgatatg
     1981 aattttgagc caggcattga cctgaaaact atcatcttgg ttgttgaaga cgaagcagca
     2041 attcgtttgg ggctttctgc tgccatgtcc cgtcagggat accgtgtgat cactgcagaa
     2101 aatgggaatg atgccatcca aaaggcgggc gaatatcacc ctgacctgat catttcggat
     2161 gtcatgatgc ccgtattgga tggctttgag atgaagaaga agctgtctgc tgacccggat
     2221 ctggcgtcta ttccattgat ctaccttaca gcgcgtactg cggtggaaga ccgtgtaaaa
     2281 gggattcgag atggtgcgga tgattatgtc accaagcctt ttgatattga tgaattggcg
     2341 gctcgggtcg aagcggttct tcgacgggtg cgtagtgagc gcaagcgcgg gcatgaacaa
     2401 gccaaagaat ttgaccaaga cgaccttgaa aagttgcgcc gtgagttgat gcagaatttt
     2461 catcatgaga ttcgtacacc gttaaacaat gtcatgatgt ttttagagat cgtagcaacc
     2521 cataaatttg agtcgttgga agagcaaaaa gagttcgttc gtattgcccg ttccagcggt
     2581 gagcggcttg agtcactgat tagtgatatt gtgctattaa cagacctgga ccaaggtttg
     2641 cttaattgtg ttcgtcaaac cattaaccca gatatccacc ttttgcagcc aattcgtcgt
     2701 agaatctctc gatatgatgg gaaggttgtg cacttcttac cgattctttc tattaatgac
     2761 gagatcaaag cgccgcggca ggaatttcca cgcgcaattt tgcatttagt cgataatgcc
     2821 ttgaagttca gcccagaaga cggaactgtg attctcaaag taacttcaag taaaaatggg
     2881 ggagcgacca tcaccattga agaccaagga ccgggcattc cggtagggtt gcgtgaaaaa
     2941 gttttcgaac gttattatca gattagcagt ggggcaacac gattacatca agggttgggg
     3001 gtagggttaa cacttgctcg tgcggttttg cgttctttag gtggggatgt caaaattttg
     3061 gatagtgcac aaggctgttg cgtggaggca gtcttgccag accttgcgga agatgatatt
     3121 gcttatggat gaaaaagttc tcccgctcaa agccattggc gttgatataa aagacacctc
     3181 ctggaacgga gatctggatt atcgcgcctt gttcgagcag acgggcgatg gtgtttttat
     3241 tattggctta gatttcaatg tgcttgccgc aaaccctccg gcgaaccgaa tgttggggta
     3301 tgtcgatggt gagttgcaag gaaccagtgc tgaaattttc ctcaacctca agcaaggctt
     3361 tcaaaaagaa ttaatccttg aaaagcgggt taatgttttt gaacataact tgcgtaagaa
     3421 agatggttct gtcttacagg cggaacttag ttcttcactt gtttacaatg ttgaagacaa
     3481 acctgcttac attcaattga ttgcaagaga tattggggaa cgtaagaaaa atgaacgctt
     3541 gctcaaacgt aatgtgcggg ctcagtctgt cattggcgaa gtgacggcgg cgcttttccg
     3601 ttctaccaat atcgaaggca gtttgcctga ggtgcttgag tcgcttgggt atgcggtggg
     3661 tgtttttagt acgttgatat tcgaagtgaa aaattcgtcg ctgagagtaa ggcatagatg
     3721 ggtggaccca actgtccctg agtttgatgc ggagactgtc cttcaaccgt ttttggattc
     3781 gctatccaaa tttccagacc gggtcttttc gcttccagaa cttcctgaaa aagtagccag
     3841 tattccaaaa atttccatgc tggcaattcc cattcaaggg gcattgggaa cgtgggggtt
     3901 tttaggcttg tttgataaag aagaccgcct ctcttggttg cccaccagct ttgatatcgt
     3961 gcagacgact gctaatttgc tgggtgctgc aattgagcgt atgcattacg aagagacttt
     4021 gcgtttgagt gagaggcgta accgcatcat tgtggacgcg ttgcctgatc tgcttttgcg
     4081 agtggatcgt tcgggacgaa tttttgacta taacgcaacc tcttcgcacc cgttgtatct
     4141 tgagcctgcc agtgccattg gcaagaattt gtacgagttg tggaatgacg atattgctgg
     4201 aaagttgtta ccgcgccttc ctgatgagac gttgtttaat ttgccacgta ctgtggaaaa
     4261 ttttaagttg ccgggaaaat tgggctcttt tgaagcgcgc ttgtttcctc tcagtaaact
     4321 agaagcgttg attattgtgc gcgatattac agaacaggca cggcttaatc agatgaaatc
     4381 tgattttatt aactgggcat cacacgaatt acgtacccca ttaacttctg ctatcttaat
     4441 gacagaactg cttagacagg aatgcacgcc cgaagaagca gaagaatatc tttctacgct
     4501 gtctagtgaa ctgaaccgac agaagaattt gatcaatgaa ttgttgatgg ctggacgatt
     4561 ggaaaatgga accttgcatg ttgaggttgc cgcagtggac ctgcacccag tattgaaaga
     4621 tgccatttta tcggtcaaac cgattgcaag aaagagaaat attgccattc aaatggtgac
     4681 ggctgccaac ccttgttttg tcatgggaaa tgtaagtgcg cttcagcagg tgtttatcaa
     4741 tttgctaagc aacgccgtga aattctctcc ggatgatagt caggtgattt tgcgagtgga
     4801 tcgcaaagat acagaaacac atgtggcggt tgtagatagt gggttgggca ttccttctga
     4861 tgcgatgccc tatct
//
LOCUS       DAOREF010000090         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9857_length_4868_cov_4.531477, whole genome shotgun sequence.
ACCESSION   DAOREF010000090 DAOREF010000000
VERSION     DAOREF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9857_length_4868_cov_4.531477"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKI33_02815"
     CDS             <1..462
                     /locus_tag="PKI33_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35961.1"
                     /translation="NELYPKSIFFGTWLGAVIGTLLGAGSGAISGTWNGILTAAIMGL
                     VTGAINGALTGAVVVRTTGTTGGVSTGAYTGMAFGAFLGLVFGIFLPESFRQSVLKLD
                     VLILNVIFAGRFEAAILFSFLFSVLSTAVGSWIGGRNLKKRDVENLKTNRS"
     gene            578..1330
                     /locus_tag="PKI33_02820"
     CDS             578..1330
                     /locus_tag="PKI33_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNM35962.1"
                     /translation="MSGHSKWSTIKRKKGVADAKRGAIFTRLAREITIAAREGGDPDT
                     NFRLRLAVEKARAENMPKDNIERAIKKGTGEDKDAAVFEELTLEGYGPHGSAVMVSCV
                     TDNRVRTVSDVRHIFSKFGGNMAEAGAVGWQFDRKSYFSFPSSAMAFDKAFELGVEAG
                     ADDVVDSGEEIEIFGPVEAFKTIMDTLGKAKVQPAEAGLRLIAKQEIDLGVEEIMAVM
                     KVVENLEDLDDVQDVYHNVKVSDEALAALENA"
     gene            1379..1861
                     /gene="ruvC"
                     /locus_tag="PKI33_02825"
     CDS             1379..1861
                     /gene="ruvC"
                     /locus_tag="PKI33_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNM35963.1"
                     /translation="MTLALGIDPGTATTGYGFVRLMPDGELVAVDYGVILTPKEATAS
                     ARLVMLFDDLNKLLKKHKPETAAVEKLFFSKNVTTGIAVGQARGVILLALEKAGISTY
                     EYTPNEVKQAVAGYGSAEKRQVQEMVRALLQLEKIPKPDDAADALAIAITHLNTARYD
                     "
     gene            1919..2497
                     /gene="ruvA"
                     /locus_tag="PKI33_02830"
     CDS             1919..2497
                     /gene="ruvA"
                     /locus_tag="PKI33_02830"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNM35964.1"
                     /translation="MIATLRGEVSHVEDNAIIVEVGGVGMRVFVPAPMRTKASAGEMI
                     FLFTHLVVREDALTLYGFESQSDRDLFNILLGVDGVGPKVALSVLSTLTLETIQRAIF
                     ADEEELLSKVPGVGKKTAQKMAIHLKDKLKPTDALSKMAALADYDSEVLAALAALGYS
                     VVEAQAALQALPKDAPKDVEERLRVALQYIGK"
     gene            2497..3183
                     /locus_tag="PKI33_02835"
     CDS             2497..3183
                     /locus_tag="PKI33_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35965.1"
                     /translation="MALPDFLAKPIFHIVYEVMRRQRGSPIPAMTRRVQMPADEKRLA
                     FDALLDSAIQNGPSVPISYNLPYPKSEFLYYICDWRGFVVHGSPLHDLEMLEPIRKSG
                     DDNEFGNRQQIFCSPDAAWAMWFAILDKSKYGLTSNGCVRVGIGNKRVKYYHFELPKT
                     TEADKPFTDGMIYICRAEDFPDKRAFPMLDRFNGEVEEWGSTKPVKPLAKIPVSPNDF
                     PYLDKVQFSL"
     gene            complement(3243..3959)
                     /locus_tag="PKI33_02840"
     CDS             complement(3243..3959)
                     /locus_tag="PKI33_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35966.1"
                     /translation="MQSQNEFNESSVSIQSFLLFLFASVIGLLVATLVLPFVLPNMAF
                     SLSGASPKAYWYLSRGTAFVSLSLLWLSMALGLGITNKMARLWPGMPATFAIHEYVSL
                     LGLAFAAFHAVILLGDEYINFTLLQVFIPFSTVDYRPFWVGIGQLGFYIWLIVAMSFY
                     VRPMIGQKFWRVLHYLSFAMYFVGIFHAIFSGTDSSMSWAQNYYWFSAGSLLFLFFVR
                     IVSIVIDGLFPVKKPVPVQR"
     gene            complement(3943..>4868)
                     /locus_tag="PKI33_02845"
     CDS             complement(3943..>4868)
                     /locus_tag="PKI33_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNM35967.1"
                     /translation="PFRAMGSDMLVILQSDSNSAQDVLKDVPNWFEEWEQSLSRFRLD
                     SELSRLNRTFDQPVSVSNALWEVFQTAVWANGFSDGLVTPTVLDAMLDAGYDLSFNEL
                     SHEQFGMFSQTEHHPLSQIVADPENQTITLPQGVRLDFGGVAKGWAAHQAMERLREFG
                     PCLMNAGGDIAVSEPQLNGSTWQIGVSDPFHAGSDFDTLLVNQGGVASSGKDRRKWKR
                     NGLLQHHIINPNTGLPVDTDILRVTVVAATVMEAEAAAKTAFILGKERGVAWLEEQPS
                     FAGLMVMDSGEVFITSSMTEYLQGVKHAIAK"
BASE COUNT         1260 a         1265 c         1231 g         1112 t
ORIGIN      
        1 aacgaacttt atccaaaaag cattttcttt ggcacctggt tgggtgccgt cattggcacc
       61 cttcttgggg ctggctcggg tgcgatcagc ggcacatgga atgggattct caccgcagcc
      121 atcatggggc ttgtgaccgg cgccatcaac ggagctttaa ccggggcggt tgtcgtccgc
      181 actacaggga cgacgggcgg tgttagcacg ggtgcctata ctggtatggc gttcggcgcc
      241 tttttggggc tggtcttcgg catcttcttg ccggagtcct ttcgccaaag cgtcttgaaa
      301 ttggatgtgc tgattttgaa tgtcattttt gcagggcggt ttgaggcggc gattttgttc
      361 agcttcctgt tttcggttct ttccacggcg gtcggttcgt ggattggcgg acggaacctc
      421 aaaaagcgcg atgtggaaaa tctaaaaacg aatcgctcat aaggcagagt tgggacagct
      481 tctgccccta gcatgccgtg aaaccagaat ggtataattt cgccgcttaa ttcatcattc
      541 cccaacctaa attggtcatt atctgaagga gaacgacatg tccggacatt ctaaatggtc
      601 caccattaaa cgcaaaaaag gcgttgccga cgccaaacgc ggcgccatct tcacccgctt
      661 ggcgcgtgaa attaccattg ccgcccgcga aggcggcgac cccgatacca acttccgctt
      721 gcgcctggct gtagaaaaag cccgcgctga aaatatgcct aaagacaaca tcgaacgtgc
      781 cattaagaaa ggcaccggcg aagacaaaga tgccgccgtg ttcgaagaac tcactctcga
      841 aggctacggt ccgcatggct cggctgtgat ggtctcctgc gtaaccgaca accgcgtacg
      901 caccgtttcg gatgtacgtc acatcttcag caagttcggc ggcaacatgg cagaagccgg
      961 agccgtgggc tggcaatttg accgcaaatc ctacttctcc ttcccctcca gcgccatggc
     1021 gttcgataaa gcctttgaac tcggtgtgga agccggagcc gacgatgtcg tggatagcgg
     1081 cgaagagatc gaaatctttg gtcccgtaga agccttcaaa accatcatgg atacgctcgg
     1141 caaagccaaa gtgcagcctg cggaagccgg tctacgtttg atcgccaaac aagagatcga
     1201 cctcggcgtg gaagagatca tggcagtaat gaaggtcgtc gaaaacctcg aagacctcga
     1261 cgatgtgcaa gatgtctatc acaacgtcaa agtctctgac gaagcactcg cagccctcga
     1321 aaacgcgtaa ttcaatttta cgtaggggcg gggttacccc gcccctacat tatttaacat
     1381 gactctcgcc ctcggaattg accccggcac cgccaccacc ggctacggat tcgtgcgcct
     1441 catgccagac ggggaactgg tcgctgttga ttatggcgtg atcctcaccc cgaaagaagc
     1501 caccgcctcc gctcgcctgg tgatgctctt tgacgatctc aataagcttc tgaagaaaca
     1561 taaacccgaa actgctgccg tagaaaaatt atttttctcg aagaatgtca ccaccggcat
     1621 cgcggtcgga caagcccgtg gcgtgatttt gctggcactt gagaaagccg gcatttctac
     1681 ctacgaatac accccaaatg aagtcaaaca agcggtagcg gggtacggct ccgctgagaa
     1741 gcgacaagtg caagaaatgg tgcgcgcttt attgcagtta gagaaaatcc ccaaacctga
     1801 cgatgccgcc gacgcattgg caattgccat cacgcatttg aatacagctc gatatgatta
     1861 attatgtagg ggcgactcgc gagtcgcccc tacattttat ttacggtaaa atacaaacat
     1921 gattgccacc ttacgcggag aagtatcaca cgttgaagat aatgccatca tcgtcgaagt
     1981 aggcggtgtg ggcatgcgag tcttcgtccc ggcgccgatg cgcacaaagg cttcggcggg
     2041 agaaatgatc tttttgttca cccatctcgt cgtccgggaa gatgcgctga ctctttatgg
     2101 cttcgaatcc caatcagacc gcgacctctt caacatcttg ctcggcgttg atggtgtcgg
     2161 tcccaaagtg gcgctctcgg tgttatccac gctcacgttg gaaaccattc aacgtgccat
     2221 ctttgccgac gaagaagaat tgcttagcaa agttccgggt gtgggcaaaa agaccgcgca
     2281 aaaaatggcg atccacctca aagacaaact taagccgacc gatgcgcttt ccaaaatggc
     2341 agctcttgcc gactacgata gcgaagttct cgccgcgctt gccgcattgg gttactcggt
     2401 ggtcgaagcg caagccgcat tacaagcgct tcccaaagat gctcccaaag atgtcgaaga
     2461 acgtttgcgc gtggcgttgc aatacatcgg caagtaatgg cgctccccga ttttcttgct
     2521 aaacccatct ttcatatcgt gtacgaagtg atgcgccgcc agcgcgggtc tcctattccg
     2581 gcgatgactc gcagagtgca aatgccagcg gacgaaaaac gcctcgcttt tgatgccctg
     2641 cttgattcag ccattcaaaa cggacctagt gttccgatct catacaacct accttaccca
     2701 aaatctgaat tcctttatta catctgtgac tggcgtgggt ttgttgtgca tggttcgcca
     2761 ctgcatgatc ttgagatgct tgagcccatc cgtaaaagcg gcgatgataa cgagtttggc
     2821 aatcgtcagc aaattttctg ctcgcccgat gccgcctggg caatgtggtt tgccatctta
     2881 gataaaagca aatacggatt gacaagtaat ggttgtgtgc gtgtgggcat tgggaacaaa
     2941 cgagtcaagt actatcattt tgaattgccc aaaacgacag aagcagataa acctttcacc
     3001 gacggcatga tctacatctg ccgtgccgaa gactttcctg ataagcgtgc attccccatg
     3061 ctggatcgat tcaacggcga agtagaagaa tggggcagca cgaaaccggt caagccgttg
     3121 gcaaagattc ctgtttcgcc taatgatttc ccgtatttag acaaggttca attttctttg
     3181 taaatcaaaa gagactgcca cggtgaaagt gacagtctct ttatttgata tgttgctaaa
     3241 acctatcgtt gaactgggac gggtttcttg acggggaata agccatcgat cacaatgctg
     3301 acgatgcgca caaagaataa gaagagcaaa ctgccggcag agaaccaata atagttctgt
     3361 gcccaactca tgctggagtc tgttccgctg aagatggcgt ggaaaatacc cacaaaatac
     3421 atggcgaaac tcaagtagtg cagcacacgc caaaacttct gcccgatcat agggcgcaca
     3481 taaaaactca tcgccacgat caaccaaata tagaacccaa gctgaccgat ccccacccaa
     3541 aacgggcggt aatctactgt gctaaatggg atgaatactt gtaagagggt gaagttgatg
     3601 tattcatcgc ccagcaaaat cacagcatga aaggcggcaa atgccagccc taacaaactt
     3661 acatattcat gaatggcaaa ggtggcaggc ataccgggcc acaaacgagc cattttattg
     3721 gtgatgccta acccaagcgc catggaaagc cacaaaagag agagtgaaac aaaagctgtg
     3781 ccgcgcgaaa gataccaata tgctttgggt gaagctccgc tcagagagaa tgccatattg
     3841 ggcaacacaa agggcagaac caaggtggcg accaacagtc caatgacaga ggcgaagagg
     3901 aacagcaaaa acgattggat gctgaccgac gactcattga attcattttg cgattgcatg
     3961 tttaactcct tgcaaatatt cagtcatgga agaggtgata aacacttcgc cagagtccat
     4021 gaccattaaa ccagcgaaag aaggctgttc ttctaaccaa gccactccgc gttctttgcc
     4081 gagaatgaag gctgtttttg ctgcggcttc ggcttccatc acggtagctg cgaccacggt
     4141 cacacgcagg atatcggtat ctacaggcaa acctgtattg ggattgatga tgtgatgttg
     4201 aagcaaaccg tttcgcttcc attttcggcg gtctttgccc gaggaggcaa ccccgccttg
     4261 attcacaagc aaggtgtcga aatcgcttcc ggcatggaat gggtcagaga caccgatctg
     4321 ccatgtgctc ccattcaact gtggttcgct cactgcaata tcgccgccgg cattcatcag
     4381 gcatggaccg aactcacgta aacgctccat ggcttgatgt gccgcccaac ctttggctac
     4441 accgccaaag tccagccgga ctccttgggg taaggtgata gtctgattct ctgggtctgc
     4501 gacgatctgt gaaagcggat gatgttcggt ctgtgaaaac atgccgaact gttcatgcga
     4561 tagttcattg aatgaaaggt cgtagcctgc atctaacatc gcatctaaca cggtgggtgt
     4621 gaccagcccg tcactgaacc catttgccca cacggctgtt tgaaagacct cccagagcgc
     4681 attgctcacg ctcacaggtt ggtcaaaggt tcggttcagg cgagacagct cactgtccag
     4741 ccggaagcgg gaaagagatt gctcccattc ttcgaaccag ttcggcacat ctttcaaaac
     4801 atcctgtgca gaatttgaat ccgattgaag aatgacgagc atgtcactgc ccatcgcgcg
     4861 aaagggat
//
LOCUS       DAOREF010000091         4860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9885_length_4860_cov_4.786472, whole genome shotgun sequence.
ACCESSION   DAOREF010000091 DAOREF010000000
VERSION     DAOREF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9885_length_4860_cov_4.786472"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKI33_02850"
     CDS             complement(<1..439)
                     /locus_tag="PKI33_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNM35968.1"
                     /translation="MRLFKQLVAVFAVLAVLLAILMTFSYDIIKIQWVTFMAIQPSFD
                     DQEKPLAVPAQSIPVDGAAYLPGMGEPTNPVPADETSIARGKEIYQINCMMCHGETGQ
                     GNGTVAAFLVKKKPADLTSEPVQAKSDGSWFLTISNGIWNPNNT"
     gene            complement(436..978)
                     /locus_tag="PKI33_02855"
     CDS             complement(436..978)
                     /locus_tag="PKI33_02855"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNM35969.1"
                     /translation="MTETKNTVVDILAVFSDLAPAADAIEQLRALGVSDAHMNVISGV
                     PVTEAMLGRPSQWTNVPRIALGGAILGFGAGAFLAFVAPYWYPYPIQVSTQAFVPGPP
                     TVVVLFELTMLGMLLSTFLGVFLDSYFPNYRPMKYVPEISDGKIAIYVECPDCDEKKI
                     TDLLKKLGAESVKPAEAQHL"
     gene            complement(988..2415)
                     /gene="nrfD"
                     /locus_tag="PKI33_02860"
     CDS             complement(988..2415)
                     /gene="nrfD"
                     /locus_tag="PKI33_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNM35970.1"
                     /translation="MSDNKHHAAHSHEEHLREKHLMLDPLPRGRMNEMVMESMLEKPT
                     WKFWVIFGFLAFVVVYFLFYKWAILITKGQGEAGVMRPDYWGIFLVNTVFWIGISHAG
                     TFISAILRVFRAEFRRPFTRAAELMTTFGLVQAGFSIFMHMGRVWLFYWLMPYPNERM
                     LWPNLHSPLSWDLLAITTYLLSSTMYLFLPLIPDVAMARDRSTGWRKNLYKILALGFR
                     GTEGEWTHLRNAMNIFAFAIIPVMFSVHTIVSWDFAAATRPGWSSTIFGPYFIVGALH
                     SGMGAAAVVLAVIRSTMKHMDYFVRGEHFDAIGKLMLIISMAWAYFFFNDYMVQWYGG
                     DKWTQQLLHFHEAGPLGWMWFGMLFCNVVVPWAILWNSKWRSTPWAITVVGLIINVGM
                     WFERYIIIPISVTINRMPFTWRMYTPGIEVPMGIGTVALFILLYMAASKLIPLIPVWE
                     VQEGQMAHELKKFGRETVVSVSELE"
     gene            complement(2418..3143)
                     /locus_tag="PKI33_02865"
     CDS             complement(2418..3143)
                     /locus_tag="PKI33_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNM35971.1"
                     /translation="MIRPEEIKMSAEETGKWGMVINQDICTGCQACVAACAMENNVTF
                     VGEEDAGYGRSMHWIRIERFWEGEYPEVKMTPQQPMLCQQCGHATCEPVCPAFATVHS
                     QAEQLNLQVYNRCVGTRYCANNCPYQVRAFNWRDYQRPEPLPNQFNPDVTVRRMGVME
                     KCTFCIQRIHKAQDKAKAEGREVMDGEFTTACAQACPANAIVFGRVDQPESMVSQLAH
                     KGHGVKHLEELGTLPNVTYFKGG"
     gene            complement(3146..>4860)
                     /locus_tag="PKI33_02870"
     CDS             complement(3146..>4860)
                     /locus_tag="PKI33_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNM35972.1"
                     /translation="LVSDLAKETGMNAPVRYGALGMFEARATLSKAAENVLGQAGLPW
                     FDINKAQVVFSFGANFLETWISPLPYTRGFVGLREGETKLRGTFVQFEARMSATGAKA
                     DHWVPVRPGTEGLVALAIGKLVSELRGGATPRVFSGVKPAEVAEAAGVELAALEHLAE
                     MFANSNALAIPGGPALGQSNGLEIAEAVLALNALSDNFGKPGGVYISALAPNQTEYSR
                     PASAEEMAAFIEQLKSGAIKALFVHGVNPVFELPASLGFKEALANVELISFATFPDET
                     AAEAKYVLPDHHGLESWGYQRVATGVSLPVLSGAQPVVSPFYDTRATADVLIAAAQLA
                     GGKFAEALPYKDEVEFIQTKISSLLSDANGSFTATDPVTFMAYFQQYGGWYKNADSLV
                     APDGSAVLDSNIKAAEPEFAGEGEFFFVPFVSPTLAEAGANKPWLQELPDPTTTVMWN
                     TWIEMNPETAHELGIENDDVVRVSSEAGEVEAYVYLYPAIRPDTIAMPFGQGHTAYGR
                     YAEKRGVNPADVLGAHFNGAGDLAFAGMKVKVEKVSSAVRGVGLSRMESRMGVYGEAL
                     GEEH"
BASE COUNT         1310 a         1280 c         1244 g         1026 t
ORIGIN      
        1 gagtgttgtt gggattccaa ataccgttag agatcgtcag gaaccagcta ccatccgatt
       61 tagcttgaac aggttcactg gtgaggtcag caggcttctt ctttacgagg aatgctgcca
      121 cggttccgtt cccctgccca gtttcgccat ggcacatcat gcagttgatc tgatagatct
      181 cttttccacg ggcgatagag gtttcatctg caggaaccgg attggtgggc tcacccatgc
      241 caggcaaata cgccgcgcca tccactggaa tggactgtgc aggaactgcc aaaggttttt
      301 cctgatcatc aaaagagggt tgaatcgcca taaatgtcac ccactggatc ttaatgatgt
      361 catatgagaa cgtcatcaag atggcaagga ggacggcgag aacagcaaat acagcaacaa
      421 gttgtttgaa aagtctcata ggtgttgtgc ctccgcaggc ttcacagatt cagcgccaag
      481 tttctttagc aggtctgtga ttttcttctc atcgcagtct gggcactcta catagatagc
      541 gatcttgcca tcactgatct ctggcacata cttcatcgga cgatagttcg ggaagtagct
      601 atcaagaaac acaccaagga aggtggaaag aagcatgcca agcatggtta attcaaagag
      661 aacaaccaca gtaggaggac ccggtacaaa agcttgagtg ctaacttgga tcgggtacgg
      721 ataccaatag ggcgccacaa aagcgaggaa ggcacctgca ccaaaaccga gaattgcgcc
      781 acctaaagca atgcgcggca cattcgtcca ttggctgggg cgtcccaaca tagcttcggt
      841 gaccggaacg ccagagatga cattcatatg agcatcacta acgcccaagg cgcggagttg
      901 ttcaatggcg tccgcagcag gcgcgaggtc cgaaaagacc gcgagtatat cgacaacagt
      961 atttttcgtt tcagtcatcg tatcctccta ttcaagttcg ctgacagaga ccacggtctc
     1021 acgtccaaat ttcttgagtt cgtgtgccat ctgaccttcc tgcacttccc aaacagggat
     1081 aagcggaatc aacttggaag ccgccatata aagcaggatg aagagggcaa cagtaccgat
     1141 acccatgggg acttcgatac caggtgtgta catacgccaa gtgaacggca tacggttgat
     1201 ggtgacagag atcgggataa tgatgtagcg ttcgaaccac ataccaacgt tgatgatcaa
     1261 accgacaacg gtgatcgccc atggtgtgga tcgccatttg gaattccaaa ggatcgccca
     1321 aggcactaca acgttacaga acaacatacc aaaccacatc cacccgagcg gacccgcttc
     1381 atggaaatgg agtaactgct gtgtccactt atcgccacca taccactgta ccatgtaatc
     1441 gttgaagaag aagtatgccc aagccatgga aataatcagc atgagcttac caatcgcatc
     1501 gaaatgttca ccgcgcacaa agtaatccat gtgtttcatg gtgctgcgaa ttacagccaa
     1561 tacaaccgca gccgcaccca taccggaatg caaagcaccc acgataaagt acggaccaaa
     1621 gatggtggaa gaccaaccag ggcgggtcgc cgcagcaaag tcccaagaca cgatggtatg
     1681 aacagagaac atgaccggga tgatcgcaaa ggcgaaaatg ttcatcgcgt tgcggaggtg
     1741 ggtccactcc ccttctgtac cgcggaagcc aagagcaagg atcttataca aattcttgcg
     1801 ccagccagtg gagcggtcac gagccatcgc tacatcaggg atgagcggca ggaagagata
     1861 catggtggaa gaaagcaagt aagttgtgat cgccaacaag tcccatgaaa gtggagagtg
     1921 aaggttgggc cacaacatgc gttcattggg gtaaggcatc agccagtaga aaagccaaac
     1981 acgacccata tgcatgaaga tcgagaagcc agcttgcacc aaaccgaagg tggtcatcaa
     2041 ttctgccgca cgggtaaacg gacggcgaaa ttctgcccgg aacacgcgca aaatcgcgga
     2101 gatgaatgta cccgcatgac tgataccgat ccaaaacacg gtattgacca ggaagatacc
     2161 ccaatagtct gggcgcatca caccggcttc accctgacct tttgtaatga ggatcgccca
     2221 cttatagaag aggaagtaca ccactacaaa tgccaagaaa ccaaagatca cccagaattt
     2281 ccatgtgggt ttttccagca tggactccat gaccatttcg ttcatgcgac cgcgaggaag
     2341 cgggtcgagc atcaagtgtt tttcacgcag gtgttcttcg tgcgaatgcg ctgcgtgatg
     2401 tttattatct gacataatta ccctcccttg aagtaggtca cgtttggaag cgtgccaagt
     2461 tcttcaagat gtttgacgcc atgtcctttg tgagccaatt gagagaccat cgattcaggc
     2521 tggtccacac gtccgaagac aatggcgttg gcagggcagg cttgggcgca agcagtggtg
     2581 aattcaccat ccattacttc gcgtccttcg gctttggctt tatcttgcgc cttgtgaatg
     2641 cgctggatac agaaagtaca cttctccatc acacccatac gacgaacagt cacatcaggg
     2701 ttgaactgat taggaagcgg ttcagggcgc tgataatcac gccagttgaa agcacgaacc
     2761 tggtaagggc agttgtttgc gcagtaacgg gtacccacgc agcggttgta aacctgcaag
     2821 ttcaactgct cagcctgaga atggaccgtg gcaaacgccg ggcagaccgg ttcacaagtg
     2881 gcatgaccgc actgctggca cagcattggc tgttgcgggg tcatcttgac ctcaggatat
     2941 tcaccctccc aaaaacgttc aatgcggatc cagtgcatgg aacgcccata cccagcatct
     3001 tcttcaccga cgaaagtgac attattttcc attgcacaag ccgccacaca agcctggcag
     3061 ccggtacaaa tatcttggtt gataaccatt ccccatttgc cggtttcttc ggcgctcatt
     3121 ttgatttctt cgggtcgaat catgattaat gctcctcacc cagcgcctcg ccgtacacgc
     3181 ccatgcggct ttccatgcgg gaaagaccaa caccacgtac agcgctggag accttttcaa
     3241 ctttgacttt cataccagca aaagcaagat caccggctcc gttaaagtga gcgccaagca
     3301 catcagcggg gttgacccca cgcttctcag cataacgccc ataagccgta tgaccctgac
     3361 cgaacggcat tgcaatcgta tcggggcgga tcgccggata caagtacaca tacgcttcca
     3421 cttcaccggc ttcgctactg acgcgaacca catcgtcatt ttcaatgcca agttcgtgag
     3481 cggtttcagg attcatttcg atccaggtat tccacatgac cgttgtagtg gggtcaggga
     3541 gttcctgcaa ccaaggtttg ttcgcgcccg cctctgcgag agtcggagag acgaacggca
     3601 cgaaaaagaa ttcaccttcg ccagcaaatt caggctcagc ggctttgata ttgctatcaa
     3661 gcacagcaga gccatctggc gctaccaaag agtcggcgtt cttataccaa ccgccatact
     3721 gctggaagta cgccatgaag gttacagggt ctgtagcagt gaaggagcca ttcgcatcac
     3781 tcagcaaaga gctgatcttg gtctgaatga actcaacttc atctttatat ggaagggctt
     3841 cggcaaactt gccaccagcc aattgagccg cagcaattaa cacatcagcc gtggcgcgag
     3901 tatcgtagaa gggagaaaca acgggctgtg cgccagagag caccggcaaa gacacgccag
     3961 tggctacacg ctggtaaccc caagactcaa gtccgtggtg gtcgggcaac acatattttg
     4021 cctcagcggc ggtttcatcg gggaaggttg caaacgagat aagttctaca ttcgcaagcg
     4081 cttctttgaa gccgagagaa gcaggcaatt caaagaccgg gttcacaccg tgaacaaaca
     4141 aggctttgat agcgccgctc ttcaactgtt cgataaaagc agccatttct tcggcagaag
     4201 cagggcggct atattcggtc tggttcgggg cgagagcaga aatgtacacg ccaccgggtt
     4261 taccgaagtt atcagagaga gcattcaacg ccaacaccgc ttcggcaatt tccaaaccgt
     4321 tggattgacc aagggcagga ccgccaggaa tggcaagcgc gtttgaattg gcaaacattt
     4381 cagcgaggtg ttccaacgcc gccaactcga cgccagctgc ttcagcgact tcagccggtt
     4441 tcacgccgga gaagacgcgt ggtgttgcgc cgccgcgaag ctctgagacg agtttaccga
     4501 ttgcaagggc aaccaagcct tcggtcccgg ggcgaaccgg cacccaatgg tcagccttgg
     4561 caccagtagc agacatcctc gcttcaaatt gaacgaaggt gccgcgcagt tttgtctcgc
     4621 cttcgcgcaa gccgacgaag ccgcgagtgt aaggcaacgg agaaatccag gtttcgagga
     4681 agttggcacc aaaggagaaa accacctgcg ccttgttgat gtcaaaccag ggtaagccag
     4741 cctgacccaa aacattttca gcggctttgc taagggtggc gcgggcttcg aacatgccaa
     4801 gcgccccata gcggacagga gcattcatgc cggtttcttt tgcaaggtca gagacaaggt
//
LOCUS       DAOREF010000092         4853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9915_length_4853_cov_3.716340, whole genome shotgun sequence.
ACCESSION   DAOREF010000092 DAOREF010000000
VERSION     DAOREF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9915_length_4853_cov_3.716340"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKI33_02875"
     CDS             <1..824
                     /locus_tag="PKI33_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNM35973.1"
                     /translation="DELGIVVTVLGSGGIRPDPLDSGLHWIVPFAERVERYPKTNQTY
                     TMTSVADEGQVAGDDSIQVRTKDGQQVYIDASVIYAVDPIKVVQLYSTWRFSYEDQFV
                     RTQARGVIRDVASQYGVEEIVSSKRAEMEQSIDDQLVAIFAENNLIMRDFVLRNIRFS
                     DEYAAAVEQKQIAEQQAQQAKFVVEQKKQEAEQARQVAQGSADAAVIAAKGEAEALLI
                     QAQAQAEANQVIAQSLTPELVQYQYVQKLSPNVQTIFIPSGNQFILPLPSSPQGQ"
     gene            882..1940
                     /gene="selD"
                     /locus_tag="PKI33_02880"
     CDS             882..1940
                     /gene="selD"
                     /locus_tag="PKI33_02880"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:942..944,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNM35974.1"
                     /translation="MSIPQSGREVRLTTLSACAGUASKLSADALAQVLRPVKDIFDPA
                     AYPDLMVGLESPDDAAIWRLSDEQAIVVTTDFFTPVVDTPYEYGAIAAANSLSDVYAM
                     GGRPFLALNIAALPDHLPAEISGEILRGGAEKAREAGVVIAGGHTVKDKEPKYGLVVI
                     GFVHPQKVISKGGLKAGDVLVLTKPLGAGVTTTALKQEKADEAHVLEAIEWMARLNKN
                     AGMLASEFGVRGGTDITGFGLLGHASEMAEASHVALHFDYSKIPFLSGARGYAERGIF
                     PGGAFDNKSHFEALVKFSAEISEPAQMLLFDPQTSGGLLLGLAKEKLSAFEARAKEIN
                     QPYWVIGEAREGEGIEVR"
     gene            1942..2088
                     /locus_tag="PKI33_02885"
     CDS             1942..2088
                     /locus_tag="PKI33_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35975.1"
                     /translation="MFRVGVLELGVTCAFVALTIIIPLLIARGYAQINKRLKNIENTI
                     AKKK"
     gene            2280..3572
                     /locus_tag="PKI33_02890"
     CDS             2280..3572
                     /locus_tag="PKI33_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNM35976.1"
                     /translation="MSISSEIITIIILIVINGVLAMSEAALVASRKARLQQQASEGNK
                     ASALAVKLIEDPNIFLSAVQIGITLIGVLSGAVGGATIAEKLAVFFEKIPYIGPYSES
                     VSLGLVVLVITVLTIWLGELVPKRLGINSPERIAQIVAGPMLFISRVFSPLIKLMSNA
                     TNFVLRIFGVNPSVEPPITEEELQLLIDQGTQAGVFEEAEQDMVEGIFSLGDTRVYSV
                     MTPRTEIVWLDVSDSTEEILEKMSGSPYSRFPVRQDSLETIVGIVKSRDLLVSILSQK
                     QINLKELAKPAHFIPETMLASRALEVLKKNNAEMLLVVDEFGGVQGLLTINDILEEIV
                     GVMEGDEPQATQRQDGSWLLDGMLEVDEFKEIFNLNELPHETEYETLSGFIMTSLGRL
                     PQTADHFEWNNLRFEVVDMDGRRVDKVLVTAKQKTGTD"
     gene            3636..4799
                     /locus_tag="PKI33_02895"
     CDS             3636..4799
                     /locus_tag="PKI33_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM35977.1"
                     /translation="MSPESSPSKTKVCPTCGTRLSENAVRCLVCGTEFGAQPAPKAVK
                     KTEKSVQAASMPQLTLSLPAAIGSLLAVIAVAAGLTFFLVPKDPETFSNVETSTPTET
                     PTATIPATATLPATETPTPTLQPPFEYTVSAADGSCSQIAFNFNVSVQSIIITNNLPS
                     SCPISVGQKLMIPYPTPTVPPPATNTPPPDVATQQACETVQITVQENDTLSSIAANYA
                     VSMDAIKEFNGLTTDNVFLGQVVQIPLCERAPDPNKPTPTATLPPPYPAPNLLLPADG
                     AAFTLANDVVTLQWASVGVLRDGEAYQVVVEDVTSNQTRRITDYVTDTKYIVPSSFRP
                     KDNVAHVMRWSVVPVLQSGVDDQGKPIWIASGATSERRVFTWVGVAVQGTPNP"
BASE COUNT         1249 a         1201 c         1213 g         1190 t
ORIGIN      
        1 cagatgaact cggtatcgtt gtgacggtgc taggctctgg cggtattcgc ccagatccgc
       61 ttgattcggg gttgcattgg attgtgcctt ttgctgaacg cgtagagcgg taccccaaaa
      121 caaaccagac gtatacaatg acgtcagtag ccgatgaggg gcaagttgcc ggggatgact
      181 ctattcaggt ccgcacgaaa gatggtcagc aggtttatat tgacgcttcc gtcatttacg
      241 ctgtagaccc gatcaaagtg gtgcagttgt attccacttg gcggtttagc tatgaagacc
      301 aatttgtgcg cactcaggca cgtggcgtca tccgtgatgt ggcatcacaa tatggcgtag
      361 aagaaatcgt cagctcgaag cgcgctgaaa tggaacaatc gattgatgat caattagtgg
      421 ctatctttgc tgaaaacaac ctgattatgc gcgattttgt tttgcgcaac atccgcttca
      481 gtgatgaata cgccgcagcg gttgagcaga agcaaatcgc tgagcaacag gcacaacaag
      541 caaaatttgt agtggagcag aagaagcagg aagctgaaca ggctcgtcag gtggcgcaag
      601 gttctgcaga tgccgccgtg atcgcagcga aaggtgaggc tgaggcgttg ttgattcagg
      661 ctcaggcgca ggcagaggcg aaccaagtca tcgctcaatc actcacgccg gaactggttc
      721 aatatcagta cgtgcagaag ctttcgccga acgttcagac gatcttcatc ccaagcggca
      781 atcaatttat tctgccgttg cccagttcac cccaagggca gtaatcgagt acactacaga
      841 cctgacggtt cccgtcaggt ctgtaatcta taaaggagca tatgtccatt ccacaaagcg
      901 gacgtgaggt gcgcctcaca actctttcag cttgtgctgg ttgagcgtct aaactaagcg
      961 ccgatgcgct ggcgcaggtt ctgcgccctg tcaaagatat ctttgaccct gctgcatatc
     1021 ccgacttgat ggtgggtctt gagtcgccgg atgatgcggc aatttggcgt ttaagtgacg
     1081 agcaagcgat tgtggtaacg acagatttct tcactcctgt tgtagacact ccttatgaat
     1141 acggcgcgat tgccgcagca aatagcctct cagatgtcta tgccatgggc ggacgtccgt
     1201 tcttggcgtt gaatattgcg gcattgccag atcacctacc ggcggagatt tcaggcgaaa
     1261 ttttgcgtgg tggtgccgag aaagcccgtg aagcgggagt cgttattgca ggtgggcata
     1321 cggtcaaaga taaagaacca aaatacggct tggtggtgat cgggtttgtg catccacaaa
     1381 aagtgattag caagggtggc ttgaaagccg gcgatgtact tgtgcttacc aaaccgctcg
     1441 gcgctggagt caccacgacg gcgctaaaac aagaaaaagc agatgaggct catgttcttg
     1501 aagccattga atggatggcg cgtttgaaca aaaatgccgg gatgcttgct tctgaatttg
     1561 gcgtccgcgg cgggacggat atcactggct tcggtttgct gggtcatgcc tcagagatgg
     1621 cagaagcatc tcatgtggca ttgcattttg attattcgaa aatcccattt ttgagtggcg
     1681 cacgtggtta tgctgagcgt ggaattttcc ctggtggagc gtttgataac aaaagtcatt
     1741 ttgaagctct ggtgaagttc tctgctgaaa taagtgaacc tgctcaaatg cttttgttcg
     1801 acccgcaaac tagcggcgga ttattgcttg gtttggcaaa agaaaaactg tccgcttttg
     1861 aagcgcgggc aaaggaaata aaccaaccgt attgggtaat tggcgaagca cgcgaaggcg
     1921 aaggcatcga ggtccgttag tatgttccga gttggtgtgc tggagttggg tgtcacttgt
     1981 gcatttgtgg cgttgacaat tattattcct ttattgattg cccgtggata tgcacaaatc
     2041 aacaaacgtt tgaagaatat tgaaaatact atcgcaaaaa agaagtaact ttcaggtaca
     2101 ataagggcga cttacttagc acgaaacatc atgtccgaca ttcccataga accagaccgt
     2161 agttccccct gtccgtttca attagcttgg tggaaatctc cacgccgcga agttctttat
     2221 gcgcggcttg tatcttttta agcagtatca aacgaaattc tagagtccca taggaagcaa
     2281 tgagcattag ttctgaaatc attaccatca taatcttgat cgtcattaat ggcgttttag
     2341 caatgtctga agcggcattg gttgcctcac gcaaggcgcg cttgcaacaa caggcaagtg
     2401 aaggcaataa agcttcggca ttggctgtta agttaataga agaccccaat atatttttat
     2461 cggcagtgca aattggcatc accttgatcg gcgtgctttc tggtgcggtc ggtggtgcga
     2521 ccattgccga gaagttggct gtcttctttg agaaaatccc atatatcgga ccctatagtg
     2581 aatccgtttc attagggctt gtggtacttg ttatcaccgt gctcaccatt tggttgggcg
     2641 aactcgttcc gaagcgactt ggcattaaca gcccagaacg gatcgctcag atcgtagctg
     2701 gaccgatgct cttcatttcg cgggtgttct caccattgat caaattaatg agcaacgcta
     2761 cgaattttgt cttgcggata tttggcgtga atccttcggt cgaaccaccc attacagaag
     2821 aagaacttca attgctaatt gaccaaggca cacaagccgg cgtttttgaa gaagcggaac
     2881 aagatatggt cgaaggcatt ttcagccttg gcgatacccg cgtctattcg gtgatgactc
     2941 cacgtactga aatcgtttgg ctggatgtaa gcgactcaac ggaagagatt ctcgaaaaga
     3001 tgagcggcag tccatactca cgctttccgg tacgccaaga ctcattagag acgattgttg
     3061 gcattgttaa atcacgagat ttgttggtgt ccattctttc tcaaaagcag atcaacctga
     3121 aagaacttgc caagcctgcg cacttcatcc ctgaaaccat gttggcttca cgtgccttag
     3181 aggtgctaaa aaagaataac gctgaaatgt tactcgtcgt ggatgaattt ggcggcgtac
     3241 aaggtttgct gacgatcaat gacatcctcg aagaaattgt cggcgtgatg gaaggtgacg
     3301 aaccgcaagc cacacaacgt caagatggtt cgtggttatt agatggcatg cttgaagtgg
     3361 acgaattcaa agagattttc aatctaaacg aattaccgca cgaaaccgag tatgaaaccc
     3421 tgagcggatt cattatgact tcgctcggtc ggctgccaca aaccgcagac cacttcgagt
     3481 ggaacaacct ccgctttgaa gtggtagata tggatgggcg ccgtgtcgat aaagttttag
     3541 tcacggcaaa gcaaaaaaca ggcacagact aagcccgttc gtagctaaat cgcctaaaaa
     3601 tcgtagattt gttcgatggt ataattttcg cccgtatgag tcctgaatca tctcccagca
     3661 aaacaaaggt ctgtcctacc tgtggaacac gcctaagtga gaatgccgtc cgctgtttgg
     3721 tatgcggaac ggaatttggc gcacagcctg cacccaaagc agtgaagaaa acggaaaaat
     3781 ccgttcaagc tgccagcatg ccacagctta cgctcagcct tcccgcagcc attggctcac
     3841 ttctggctgt gattgcagtt gcagcaggtc ttacattctt cctcgttcct aaagacccgg
     3901 aaactttttc caatgtagag acctccaccc ccacagaaac ccccaccgca accatccctg
     3961 caaccgcaac cttacctgct acagaaacac ccacgcccac tctgcagcct ccctttgaat
     4021 acacggtttc ggcagcagat ggttcttgtt cacagatcgc gttcaacttc aacgtatctg
     4081 tacaaagcat tatcattacc aacaacttac cttccagttg tccaatttcg gtggggcaaa
     4141 aattgatgat tccgtacccc accccaacgg ttccgccccc agccaccaac acaccgccgc
     4201 cagatgttgc aacccaacaa gcctgcgaaa ccgtacaaat caccgtccaa gaaaacgata
     4261 ctctttctag catcgctgca aactatgccg tttccatgga tgccatcaaa gagttcaacg
     4321 gtctaacaac cgataacgtc ttccttgggc aagtggttca gattccgctc tgcgaacgcg
     4381 caccggatcc caataaacct acccccactg cgacgcttcc gcctccttac cccgccccga
     4441 acctgctcct gcctgcggac ggcgctgcct tcaccctggc aaacgatgtt gtcaccttgc
     4501 aatgggcatc tgtcggtgtg ctgcgtgatg gagaagccta tcaggtcgtg gtcgaagatg
     4561 taacctccaa ccagactcgc cgcatcacag actatgtgac cgacacaaaa tacatcgtgc
     4621 cgtccagctt ccgcccgaaa gacaacgtag cgcacgttat gcgctggtca gttgtgcccg
     4681 ttctgcaatc aggcgtggat gaccaaggca aaccgatctg gatcgcctcc ggcgcaacca
     4741 gcgaaagacg tgtcttcact tgggtcggag tcgcagttca gggaacgccg aatccataac
     4801 aggtccagca cgaagcgaaa attattaact ccctgtccgc ttgggcaggg agt
//
LOCUS       DAOREF010000093         4850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9925_length_4850_cov_3.440042, whole genome shotgun sequence.
ACCESSION   DAOREF010000093 DAOREF010000000
VERSION     DAOREF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9925_length_4850_cov_3.440042"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2295)
                     /locus_tag="PKI33_02900"
     CDS             complement(<1..2295)
                     /locus_tag="PKI33_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM35978.1"
                     /translation="MIASRWKKVWSDFWSNRGRTFLTIATIAVGTFAVGFNSNLGLYM
                     NESMDGDFLSANPSEAEIYASGLNDDLVKKAREVPGVEAVEGYSSVQGQLIRPDGTKI
                     DISFTAAETPNALTLNFLKPAMGETSIPSYGKKEVILDASASSLGYTAGDTIVIEFDN
                     GKRRELTFTGYMHAVTGFPYNLAQQVNAYVTPDTLEWLGGNINQYGRLSISVSEKQTD
                     ADHVTAVAQAVADELKRAGSTVYFVSVYQPGHHFAYSITQGVFFVLGVLGYLTVLLSG
                     FLIVNTITALMTQHTRQIGIMKATGGSAWQIFFMYLVLIMSFGIGALLLAVPLADIAA
                     QTIGSGMASWLNFYPTDYKGYSITLIQQVIVAILVPLLAAILPIYNSVRITVREALSD
                     YGLGGNVKTKEETVGKNSVLVPRPIRISLRNTFRRKTRLGLTLFTLVLGGAVFIAVYN
                     LWASFDKTIEDIQGYFLADINISFDKGYRYDKVASIATSIPGVEGAEGWLEWTGTLEM
                     NDDEAGTQILFVAPPSNSKLIDPIITSGRWLQPGDENAIVIGNHLLQIFPDLKVGDWL
                     NIDIDGKKTKWQIVGTYSITGNVSPPLLYVNYEYLSVLVGEPEMAYSLRIVTSQHDSA
                     TQNRINDQIQAAFEERGIQISSTQLGADFIRDQKAQTDVLVYFMLVMASLIAFVGGLG
                     LMGTMSINVLERTREIGVMRAIGASNGDIQAIVITEGMVIGILSWAISIFISIPITNV
                     LCYGVGMAILTAPMPAIYGMTGGQM"
     gene            complement(2292..3434)
                     /locus_tag="PKI33_02905"
     CDS             complement(2292..3434)
                     /locus_tag="PKI33_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM35979.1"
                     /translation="MAISFFKPKAEDSDTAERPIIDIRSVNKFYKTAVGDYHALSNVD
                     LQIQAGEFVSIIGKSGSGKTTLLNMITGIDRPSNGEVWVNGTGVHTLNENKMARWRGK
                     NLGVVFQFFQLLPMISVIENVMLPMDFCRMYSPVERRKRAMDLLELVELADHAHKLPT
                     ALSGGQQQRVAIARALANDPPVIIADEPTGNLDSKTAESVFVLFNDLVAKGKTIIIVT
                     HDSAMAKRTHRTALIADGEIVNEYVAKAMPTLSHTQLLQATKSTTPMTFEAGAMILSE
                     GTNADTFYIVSHGTVEVILPRPNQSDVVAVQLGPGKVFGEMEFFHEKKHRASIRASEA
                     GPVEVLAIGYDKLTELLSQSDVTREALREMAGKHNGENLSWRGVSS"
     gene            complement(3480..4601)
                     /locus_tag="PKI33_02910"
     CDS             complement(3480..4601)
                     /locus_tag="PKI33_02910"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNM35980.1"
                     /translation="MKSPKLIITILLALALTACGNASAATPESTPIPVVEADDTIIAE
                     GKLEPITYTEISLNTSGLVSELPVEEGDRVEAGDVLAVVRSENAQSLEDAQAKATQEL
                     TEAYKEYRDAQSRLDDFDVPARWNGMTPTQAIASSLEKLDVARTEFEPYKHLSEKLIK
                     YEEPKPDFSEMTQEEMEFYEASLENHKNDDRPLNGEARAKKKALDEAWELYRISIRWL
                     ELESNYQNAKVRLANAQADFDALQDTDFSLDTAALRAILANAELRAPHSGVITDLNLK
                     VGEFATDAAPVATIADTSKWLVKTSDLTEIDVVNLEEGQPVTVKLDAIPGKEFKGNVL
                     SIGQNYSENQGDVVYEVTILLSEFDPSMRWGMTAVVTFE"
BASE COUNT         1214 a         1162 c         1136 g         1338 t
ORIGIN      
        1 catttgcccg ccggtcatgc cgtaaatggc aggcatggga gcggtgagaa ttgccatgcc
       61 aaccccatag cacaagacat ttgtgatggg aattgaaatg aagatgctaa ttgcccaact
      121 caggatgccg attaccattc cttctgtgat gacaatcgcc tgaatatcgc cgttgcttgc
      181 gccaatcgcg cgcatcacac cgatttcacg agtgcgttcc aacacattga tgctcatggt
      241 gcccatcaag cctaaaccac cgacgaaagc aatcaatgat gccatgacca gcatgaaata
      301 tacgagaacg tctgtctgtg ctttctggtc gcggatgaaa tctgcgccta attgcgtgga
      361 gctgatctga atgccgcgtt cttcgaatgc cgcttgaatc tggtcattga tacggttttg
      421 agtggcagaa tcatgttgac ttgtgacgat acggagggag tatgccattt caggttcgcc
      481 gaccaaaacg cttaagtact cataattcac atataacaat ggcgggctga cgttgccagt
      541 gatgctataa gtgccgacaa tttgccattt tgtcttttta ccgtcaatat caatgttgag
      601 ccaatcacca actttcaggt ctggaaagat ttgcaagaga tgattgccga tcacaatcgc
      661 attctcatca ccgggctgca accagcgacc agaagtgata atggggtcga tcaatttcga
      721 gttagaagga ggcgccacaa ataatatttg agttccggct tcgtcgtcat tcatttcaag
      781 ggtgccggtc cattccagcc atccctctgc accttctact ccaggaatgc ttgtggcaat
      841 gctggcgacc ttatcgtatc gatagccttt atcaaacgaa atgttgatgt cagccagaaa
      901 gtacccttga atatcttcga ttgttttatc aaaggatgcc cacaagttgt aaacagcaat
      961 gaatacagcc ccgccaagta ctagtgtgaa gagggttaga cctaggcggg tttttcttcg
     1021 aaatgtattg cgcaaggaaa tgcggatcgg gcgtggaacc aacaccgaat ttttgcctac
     1081 agtctcttct tttgtcttca cgttaccccc taaaccataa tcgctcaacg cttcacgaac
     1141 tgtaattctg acgctgttat agattggcag gatggcggct aataacggaa ccaagattgc
     1201 aacgataact tgttgaatca aagtaatgga atagccctta tagtctgttg ggtaaaagtt
     1261 caaccaagat gccattccac taccgatggt ttgggcagca atatcggcaa gcggaacagc
     1321 cagcaagagc gctccaatgc caaaactcat gatgagcacc aaatacatga aaaaaatctg
     1381 ccaggcactt ccgccagttg ctttcatgat gccgatttga cgtgtgtgtt gagtcatcaa
     1441 tgcagtgatg gtgttgacga tcaaaaatcc gctaagcaat acggtgagat atcccaaaac
     1501 gcccaacacg aagaacacac cttgtgtaat gctgtaggca aagtgatgcc ccggttgata
     1561 cacacttaca aaatacaccg tagaccctgc tcgcttaagc tcatcagcaa ctgcctgagc
     1621 tacagcagtg acatggtctg cgtctgtctg cttttcagaa acactgatcg agagtcttcc
     1681 atattgattt atgtttccgc ctaaccactc taatgtgtct ggcgtaacgt atgcatttac
     1741 ttgttgcgct aagttgtagg ggaaacccgt cacggcatgc atatacccag taaaggtcag
     1801 ctcgcggcgt ttgccattat cgaactcgat aacaatcgta tctcccgctg tatatcccag
     1861 agacgaagcg ctagcatcaa ggatgacttc ctttttgcca taactgggaa tactcgtttc
     1921 tcccattgca ggtttgagaa aatttaatgt caacgcattg ggggtttctg ccgcagtgaa
     1981 agagatatct attttcgttc catcagggcg aataagttgc ccctgtacac tagaataccc
     2041 ttccactgct tcaacaccgg gtacttcgcg cgccttcttg actaagtcat catttaagcc
     2101 cgaagcataa atttctgcct cagaagggtt ggcagacaga aaatccccat ccatactttc
     2161 attcatgtac aagccaagat tgctattgaa ccctacagca aacgtcccaa cagcaattgt
     2221 ggcaatggtt aagaaggtcc gcccgcggtt gctccaaaaa tcagaccaga cttttttcca
     2281 acgactcgcg atcatgatga gactccccgc caagacagat tctcgccatt gtgtttgcct
     2341 gccatctctc tcagcgcttc acgcgtaacg tcagactgac tcagcaattc tgtgagtttg
     2401 tcatacccga tagcaagaac ctctacaggt cccgcttccg aggcgcggat ggaagcacga
     2461 tgcttcttct cgtggaaaaa ttccatttcg ccgaacactt ttccaggtcc gagttgaacc
     2521 gccacgacat ccgattgatt cgggcgcggc aaaataactt ccaccgttcc atgtgagacg
     2581 atgtagaacg tatctgcatt tgtcccttca gaaaggatca tggcacctgc ttcgaaggtc
     2641 atgggagtgg tagattttgt tgcctgtaaa agttgagtat gagaaagtgt gggcattgcc
     2701 tttgccacgt actcgttgac aatttcgccg tctgcgatga gcgcggtgcg atgggtgcgt
     2761 tttgccatgg cgctatcatg tgtgacgatg ataatggttt tgccttttgc gacaaggtca
     2821 ttgaatagca caaaaacaga ctcggctgtt tttgagtcaa ggttgcctgt tggttcatct
     2881 gcaataatca cgggcggatc attagcaagc gcacgcgcta tcgctacacg ttgctgttgt
     2941 ccacctgaaa gcgcggtggg gagcttatgt gcgtggtctg ccagttctac caattcaagc
     3001 aagtccatgg cgcgtttgcg gcgttcgaca ggggagtaca tacggcagaa atccatgggc
     3061 agcataacgt tttcgatcac agagatcatc ggcaaaagtt gaaagaactg gaatacaacg
     3121 cccagattct ttccacgcca acgtgccatt ttattttcat tgagcgtatg cacacctgtg
     3181 ccattgaccc atacttcgcc atttgatggg cgatcaatgc cggtgatcat gttcaataga
     3241 gttgtcttac cgctgcccga tttgccgata atgctgacga actcgcctgc ctgaatttgc
     3301 agatctacat tgctgagcgc gtgataatcg ccaacagcgg ttttgtagaa cttgttcaca
     3361 ctgcgaatat caatgatcgg gcgttctgcg gtgtcgctgt cttccgcttt cggtttgaag
     3421 aaggatatag ccattagtaa tttccttcaa cacgacatct atatgccgtg ttgactactt
     3481 tattcgaagg taacgacagc ggtcatgccc cagcgcatag acgggtcgaa ctcgctcaaa
     3541 agaatggtga cttcatagac cacgtcgccc tgattttcag agtaattctg accgatggag
     3601 agtacattgc ccttgaattc tttgccggga atggcatcca acttaacggt gacgggctgt
     3661 ccttcttcaa ggttgaccac atcgatctcg gtcaggtcac tggttttgac gagccatttg
     3721 gacgtatctg caatagttgc tacgggtgct gcatcagtcg caaattcacc aactttgaga
     3781 ttcaagtctg taatgacacc tgaatgaggt gcacgtagtt cagcgttcgc aagaatggcg
     3841 cgtagagccg cggtatcgag tgagaaatct gtatcttgca gagcgtcaaa gtctgcttga
     3901 gcattggcaa gccgaacctt tgcattttga tagttgcttt ccaattccag ccagcgaata
     3961 gagatgcggt ataactccca agcttcatcg agtgcctttt tctttgcgcg cgcttcgcca
     4021 ttcaagggac ggtcatcgtt tttatgattt tcaaggctgg cttcgtagaa ctccatctct
     4081 tcctgagtca tttcagagaa atcgggtttg ggttcttcgt acttgatcaa tttctctgag
     4141 agatgcttgt atggctcaaa ttctgtacgg gcaacatcca acttttcgag agaggaagca
     4201 atcgcttggg taggtgtcat accattccag cgtgcaggga catcaaaatc atccaagcgg
     4261 ctttgggcgt cgcgatattc tttgtaggct tcggtcagtt cttgggtagc ttttgcttga
     4321 gcatcttcga gagattgcgc attttcagaa cgcacaacag caagcacatc acctgcttcc
     4381 acacgatctc cttcttccac gggtaattcg ctgaccagtc cgctcgtgtt tagggagatt
     4441 tctgtatagg taataggctc cagtttccct tcggcgataa tggtatcgtc tgcttcaacg
     4501 acgggaatgg gagtgctttc tggtgttgct gctgaggcat ttccgcaggc tgtgagagct
     4561 agtgcaagta agatcgtgat gataagttta ggcgatttca tgtccgcttc ctttgcaagg
     4621 attgattatt tgttatgacc ggatgtatag aaaaaagttt caaattttgg tgaacttttc
     4681 gtgtttactt acgatgccat gggaagtttg gttgcacaaa aaagagcaca atctaaaagg
     4741 attgtgctct ttgcgtatta cttctctatt atttttttcc acccttgaga actgttttat
     4801 ttttccaaca tcatgctggc ttcttcattg agccagcgcc gcccatcacg
//
LOCUS       DAOREF010000094         4845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9939_length_4845_cov_5.134656, whole genome shotgun sequence.
ACCESSION   DAOREF010000094 DAOREF010000000
VERSION     DAOREF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9939_length_4845_cov_5.134656"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKI33_02915"
     CDS             complement(<1..319)
                     /locus_tag="PKI33_02915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM35981.1"
                     /translation="MTRTVLITGAGGGIGRACINHFSSHGWRVIGVDRSDFGDNFPKD
                     GRFIKADISQPDSVEQIFQQAKEFHPALDALVNNAAVQVAKPLVDTTVEEWDSVMASN
                     LRSV"
     gene            complement(438..2000)
                     /locus_tag="PKI33_02920"
     CDS             complement(438..2000)
                     /locus_tag="PKI33_02920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM35982.1"
                     /translation="MKQLRLVLPYVFLFLLTALVLDLANPFFDKLARDGGFFLYAGQQ
                     IVEGKLPYRDIWDSKGPGIFYINALGLLLGGGSRWGVWLVEFLCIFGMFWLLYNSLSK
                     RWGIGAALFGAALAGLGLRTVLGYGNYTEEYALLFNVAGIVLFLSMRDVERNYWKYIW
                     IGAFFGLSFTFRANNIGGLFAIFGAVFLYYLIKQHFSEALRIILAALAGFALPLIVWA
                     LYFAVMGSAGEMFYASILFNFSYSAAKDREWLDIFGGFGKYGMGWVGWFTLVAWFLAG
                     FRMLVNFVQRKVTVFDTFMLLWFPIEIILSNLSGRNFTHYYISWVLAVAVYSAFVFAE
                     FWQLTFRVPSLEGWSDGFSVAASAGLIVMLFAISPATWGRYGESIRVGGEYIDPVSAY
                     IRDNSKPGDKLLTWYPEMGVNFMSGRASPVKYVYYPLFLEGSLTKDIEDSYFADITSN
                     PPELILDCSHSVDAIPSLDAATREEQYSTPGVKKKMYIQPNMNDIFEFVEQNYTLENT
                     IETCLVFRLKKD"
     gene            complement(2024..>2398)
                     /locus_tag="PKI33_02925"
     CDS             complement(2024..>2398)
                     /locus_tag="PKI33_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM35983.1"
                     /translation="EMTDLDTLLANSDFVSVNCDLNSTSHHLINANTLSKMKSTAVLI
                     NTARGPIVDEKALVAALSSGQVGGAALDVFEFEPLPLNSPLLKMDNVMLAPHNSNSSP
                     TAWERIHWNTIKNLVEGLGIVS"
     assembly_gap    2399..2498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2499..2815)
                     /locus_tag="PKI33_02930"
     CDS             complement(<2499..2815)
                     /locus_tag="PKI33_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35984.1"
                     /translation="MKYTVLLTAPYMLPFVDRFKPVFDSYDIELIIPNVEERMEEEDI
                     LKYAGQFDGTVCGDDRYSAHVIEACLPRLKVISKWGTGVDSIDAEACSRFGVKLGRTP
                     NAFT"
     gene            complement(2819..4348)
                     /locus_tag="PKI33_02935"
     CDS             complement(2819..4348)
                     /locus_tag="PKI33_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35985.1"
                     /translation="MKKFLSRLFNAPWYSIVFGAYPILALLSENKGQVEPSAIFRPLL
                     LSLSFSTIILLIAWLVLRKIHKAAFFSVLFLLLFFTYGHVYMYFEEKFPENTAVQWLW
                     VMWVILFVLSIAWVTRPKLTFETAASSLNGMILVLLIMSITQLDFNTSSGNSHRVGAR
                     NAPIQEDLVLPENPPDVYFFLLDSYGRSDLLQSAYGYDNSDFENALKERGFYIANESQ
                     SNYVRTEISLGSSLNMQYLQELDPAFNPQSIGRKVLWDALKHNAVRYNFDTLGYETIN
                     YASGFAWLEVTDSDQFISPPPISSGMTEFEGLFLRTTWARYAEKWGWINSDAIMAEGY
                     RDRFNTIFNSMDSISKMPEPKFVYAHVISPHPPFVFDPEGNPTNPADFWNEKRQYTGE
                     MYKKGYLNQLPYLNTRMLEAIDTIMANSKTPPVIIIQGDHGPWLQPKDKRMWNFTAVY
                     MPGHEDTLYPTITPVNLFRLVFNNYFGGKYDILKDVSYFSPVPKLYEFTEIPNPYDEG
                     H"
     gene            complement(4366..>4845)
                     /locus_tag="PKI33_02940"
     CDS             complement(4366..>4845)
                     /locus_tag="PKI33_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35986.1"
                     /translation="QSSNDSCVRFIDGRSPELNSYDDGRVAMIASYSKLDSVVTEGDV
                     SAVPEEIFGAEPERGWCYTYQQADLARQRGDWDAIPVLLKDALDKGYYPNDGLEWMPF
                     MQAYAVQGNVEEIKSMLKLVVADKYLRLQVCNSLTNLMQRETLNGDVSALIEKNICK"
BASE COUNT         1446 a         1138 c          993 g         1168 t
ORIGIN      
        1 atactgaacg caaattcgat gccatcaccg aatcccattc ttctacggtg gtatctacta
       61 atggcttggc tacttgcaca gcagcgttgt tgaccaatgc atccaaagca gggtgaaatt
      121 cttttgcttg ttgaaaaatc tgttcaacag agtctggttg tgaaatatct gctttgataa
      181 aacgcccatc ttttggaaaa ttatctccaa agtcagagcg atctacgcca ataacccgcc
      241 aaccgtggct tgaaaaatga ttaatgcagg cgcgtccaat tccgccgcct gcgccagtga
      301 tgagaactgt tctagtcata gggtaatttt acgtcgaaat ctagctttgt gcctgaagca
      361 aaatgaataa aagcaacaga cgcgaatttc ccaattcgcg tctgttgctt ttactgatct
      421 atagtaattt tacaaaccta gtctttttta agcctgaaaa ctaggcaagt ctctatcgta
      481 ttttcaagtg tgtaattctg ctcaacgaac tcaaagatat cgttcatatt aggttgaata
      541 tacattttct tcttcacacc cggtgtagag tattgttcct cgcgtgttgc agcatccaaa
      601 gagggaatgg catccacaga gtgggaacaa tcgagaatca gttcaggtgg gttgcttgtg
      661 atgtctgcga agtaactatc ttcaatatct ttggtgagag acccttccaa aaagagcgga
      721 taatacacat acttcaccgg cgaagcccga cctgacatga agttcacgcc catttcggga
      781 taccatgtca acaacttgtc tcctggtttg ctattatcac gaatataagc agagacaggg
      841 tctatgtatt caccacccac acgaatcgac tcgccgtacc ttccccaagt cgcaggagat
      901 atggcaaata gcatcacgat tagccccgca gaggcagcta cactaaaccc gtcactccaa
      961 ccttccaaag atggcacgcg aaaggtcaac tgccagaact cagcaaagac aaaggcggag
     1021 tacactgcca ccgccaatac ccaactgatg taatagtgtg tgaagttcct gccagaaaga
     1081 ttgctcaaga taatttcaat ggggaaccaa agcaacataa aggtgtcaaa gaccgtcacc
     1141 ttacgttgga caaagttcac caacatacga aaaccagcca agaaccaagc cactaaggtg
     1201 aaccaaccca cccaacccat tccatatttt ccaaaaccgc cgaagatatc cagccattcg
     1261 cgatctttag cagccgagta ggagaagtta aaaagaatgg aggcgtagaa catttcacca
     1321 gcactaccca tcacggcaaa atacaatgcc catacaatga gcggcaaagc aaagccagcc
     1381 aatgcagcaa gaatgatccg cagcgcttca gaaaaatgtt gtttgatcag gtaatacaaa
     1441 aagactgcac caaaaatggc gaacaaaccg cctatgttat tggcacgaaa tgtaaaactc
     1501 agtccaaaga aagcgccaat ccaaatgtat ttccaataat ttcgttccac gtctcgcatt
     1561 gaaaggaaca gtacaatccc tgccacattg aagagcaacg catattcttc ggtgtaattt
     1621 ccgtaaccga gcacggttcg caagccaagc cctgccaatg cagccccaaa caatgcagca
     1681 ccaatgcccc accgtttgga aagtgagtta tataaaagcc aaaacattcc aaaaatacac
     1741 aaaaactcaa ctagccaaac accccaacgt gaaccgccac ccaataacaa ccccaaagca
     1801 ttgatataaa aaatgccggg tccttttgaa tcccaaatat cacgataggg cagtttgccc
     1861 tccacgatct gttgaccagc ataaaggaaa aagcccccat ctctggcaag tttatcaaag
     1921 aacggattgg caaggtctag caccaacgcc gtcagcaaga acaaaaatac ataaggcaat
     1981 acgaggcgaa gctgtttcat tatttcctca cttgcatacc ctttcaggac acaattccca
     2041 aaccttcgac caaattttta atagtgttcc aatgaatgcg ctcccatgct gtagggctcg
     2101 aattggaatt atgcggcgcc agcatgacgt tgtccatctt cagcaaagga ctattcaatg
     2161 gcagaggctc gaattcaaag acgtcaagcg ccgcgcctcc tacctgacct gaactgagtg
     2221 ccgcgaccaa ggctttttca tccacgatag gaccgcgcgc cgtattgatc agcaccgcag
     2281 tggacttcat ttttgaaagc gtattcgcat tgatcaaatg atgtgatgtt gagttgagat
     2341 cgcaattgac agagacaaaa tcagaattcg cgagcaaggt atcgaggtct gtcatctcnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt aaaagcattc ggcgttcgcc
     2521 caagcttcac cccaaaacgg gaacaagcct cggcatcgat tgaatcgact cccgttcccc
     2581 atttcgagat gactttcaag cgcggcaaac aggcttcgat cacatgtgcg ctatagcggt
     2641 catccccaca cacggtgcca tcaaattgcc cggcgtattt gaggatgtct tcttcctcca
     2701 tgcgttcttc cacgttcgga ataatcagct cgatatcgta ggagtcgaag acgggtttga
     2761 aacggtccac aaaagggagc atgtagggag ccgtgagtag aacggtgtac ttcatcgatc
     2821 aatgtccttc atcgtaaggg tttggaattt cagtgaactc atagagtttt ggaactggcg
     2881 agaaataact gacatctttg agaatatcat atttgccgcc aaaataatta ttgaagacaa
     2941 gccggaacaa gttgaccggt gtaatggtgg gatacaaagt atcttcatga ccgggcatgt
     3001 ataccgccgt gaaattccac atacgcttgt ctttaggttg caaccacgga ccgtgatcgc
     3061 cctgaataat aatcactggc ggcgttttgg aatttgccat aatcgtatca atggcttcga
     3121 gcatgcgcgt gttgagatag ggcaattgat tcaagtagcc ttttttatac atctcacctg
     3181 tgtattggcg cttctcattc caaaaatcag cgggattggt tgggttgcct tcaggatcaa
     3241 acacaaaagg cggatgcggt gaaatcacat gagcatagac aaattttggc tcaggcattt
     3301 ttgaaataga gtccatacta ttgaagatgg tattgaagcg gtcgcgatag ccttccgcca
     3361 tgatggcatc tgagttgatc cagccccatt tttcggcata tcgcgcccaa gttgtgcgca
     3421 agaacaatcc ttcaaattcg gtcatgcctg aagaaatggg cggcggactt ataaactggt
     3481 ctgaatctgt aacctctagc caggcaaagc cagaagcgta gttaatcgtt tcatacccaa
     3541 gggtgtcaaa gttataacgc actgcattgt gctttagagc atcccataat acttttctgc
     3601 caatcgattg cgggttgaat gccgggtcta actcttgaag gtattgcata ttgagtgaag
     3661 aacctaacga aatctctgtg cggacatagt tcgattggct ttcgttggcg atataaaacc
     3721 cacgttcttt cagagcattt tcaaagtcgc tattatcata tccatatgca gactggagta
     3781 agtctgaacg tccatatgaa tctagcagaa aaaaatatac atcaggtggg ttttctggca
     3841 aaacaaggtc ttcttggatg ggcgcatttc ttgcgcctac ccgatgcgag tttcctgaag
     3901 atgtattgaa atctaattga gtaatggaca taatcaacaa aaccaaaatc atgccattca
     3961 aagaagaggc ggctgtttca aaagtcaact tggggcgagt aacccaggca atagaaagaa
     4021 caaaaagaat aacccacatc acccacagcc attgaaccgc tgtattctca gggaatttct
     4081 cttcaaaata catgtataca tgcccatatg taaagaaaag cagaagaaac aaaacagaaa
     4141 agaacgcagc tttgtgtatt ttacgaagca caagccatgc aatcaaaagg ataatagtac
     4201 tgaatgacaa ggaaagcaac aaaggtctaa aaatagcgga tggctccacc tgccctttgt
     4261 tttcagagag caacgccaaa atgggatatg cgccaaaaac aatagaatac caaggcgcgt
     4321 taaacaaccg tgaaaggaac tttttcatcg ttacacctct aaaatctact tacagatatt
     4381 tttttcgatc agtgcagata catccccatt aagagtttct ctctgcatta ggtttgtgag
     4441 cgaattacaa acttgcaacc gcaagtattt atctgctacc accagcttca gcatggattt
     4501 gatctcttcc acgttgcctt ggacggcata ggcttgcata aatggcatcc attccaaacc
     4561 atcgttgggg tagtagcctt tatctaaagc atctttcaat aaaactggaa tcgcatccca
     4621 gtcacctcgt tggcgggcaa gatcagcttg ttgataggtg tagcaccagc cacgctcggg
     4681 ttctgcgcca aagatttcct ctggcacagc agacacatca ccttcggtca ctacagagtc
     4741 caattttgaa taagacgcga tcatcgccac gcgaccatca tcgtatgaat tcagttccgg
     4801 cgatctacca tcaatgaagc gtacacaact gtcattgctt gattg
//
LOCUS       DAOREF010000095         4844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9943_length_4844_cov_4.294634, whole genome shotgun sequence.
ACCESSION   DAOREF010000095 DAOREF010000000
VERSION     DAOREF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9943_length_4844_cov_4.294634"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKI33_02945"
     CDS             complement(<1..389)
                     /locus_tag="PKI33_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNM35987.1"
                     /translation="MAFNPINWLLGLFSLDIAIDLGTANTLVHVRGKGIVINEPSWVA
                     VDKKQRQPLAIGLEAKEMVGRTPANVVVVRPIRDGVIAEFDVTRVMLEYFIGKVHEQS
                     VVPLPRPRVIVGLPTGVTEVEKRAVYDA"
     gene            529..1380
                     /locus_tag="PKI33_02950"
     CDS             529..1380
                     /locus_tag="PKI33_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNM35988.1"
                     /translation="MSKFAIVTDSTSYIPTDVAQKHGITIAPQVLIWDNQTYRDGIDI
                     QPTDFYSRLKTAKSMPSTSQVSPATMQEIFQGLVEKGTSVLGIFISSKLSGTLQSAIQ
                     AKDMMGSAGEKVTLVDSRSTAMGLGFQAIIAARAAEAGASIEEAAAQASSAHERTGVF
                     FAVDTLEFLHRGGRIGGAQRFIGSALNLKPILAVKEGKVEGIERIRTKSKAHERVLEL
                     VAEQVKGKSNVRIATLHANSADDAKSLLDRAATELSPVETLFTEVSPVVGTHAGPGTV
                     GLAYIHD"
     gene            1384..2058
                     /locus_tag="PKI33_02955"
     CDS             1384..2058
                     /locus_tag="PKI33_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNM35989.1"
                     /translation="MIDPKQFERIVQSMPFLKNADSGFVKEFQKSAFLAQIPAGHDVF
                     LEGDRVEATALLISGVVRVYKIGETGREITLYRFGNGSSCILTANAILSRNTFPAIAT
                     VEQDAEAVMIPADVFRDWVKRYDLWRDFVFDLLSQRLSTVMAVVDEVVFHRMDRRVAN
                     LLLKRGKEENPIRITHQEIASDLGSSREVISRLLEDFISEGSVRSGRGIIEVLDFKTL
                     EDRSLM"
     gene            2130..2330
                     /locus_tag="PKI33_02960"
     CDS             2130..2330
                     /locus_tag="PKI33_02960"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNM35990.1"
                     /translation="MKRNLSDVDRIIRVVLAAIFAYLYFGGIVTGTFGIVLVVLAVVF
                     ALTAVVAFCPLYAPFKLSTYKK"
     gene            2383..2883
                     /gene="trxA"
                     /locus_tag="PKI33_02965"
     CDS             2383..2883
                     /gene="trxA"
                     /locus_tag="PKI33_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNM35991.1"
                     /translation="MNKQEFQEKINSSEKPLVVDFWATWCAPCMMTKPILEKLGKEYA
                     EDVEFLPINADESRDVLEDYRILGIPTVLAFKGGKEVARVTGAQNEAGYRAIFESMAK
                     GVKVKIPMTQIDRMLRLGAGGLLVMYGLPDRNWIVVAIGVVVAFLGIYDRCPIWNALT
                     KPFQRK"
     gene            2966..3589
                     /gene="udk"
                     /locus_tag="PKI33_02970"
     CDS             2966..3589
                     /gene="udk"
                     /locus_tag="PKI33_02970"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNM35992.1"
                     /translation="MMHKIPLIIGVAGGSGSGKTTVAQELLRRVGADRIAYLQHDSYY
                     KDLRMLPSTQHSEINFDHPDTLETELLIKHIIDLREFKAVQIPIYDFSTDTRKTETIT
                     VEPRNVILVEGILLYVEPELRKLFDVKLFVDADADIRFIRRLHRDITERGRSTESVIE
                     QYYATVRPMHLEFVEPSKRYADVIIPEGGHNVAALDMVTARIELLLK"
     gene            3713..4135
                     /locus_tag="PKI33_02975"
     CDS             3713..4135
                     /locus_tag="PKI33_02975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNM35993.1"
                     /translation="MVIELFADKAPVTVNNFVFLSREGYYDGVIFHRVISNFMAQGGD
                     PTGTGRGGPGYKFQDEFHPSLKHSKRGILSMANAGPGTNGSQFFITHGPTPHLDNRHT
                     VFGEVVEGEDVLMSIPERDPMKLNAPAVKIIRVTIEEI"
     gene            4113..4685
                     /locus_tag="PKI33_02980"
     CDS             4113..4685
                     /locus_tag="PKI33_02980"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNM35994.1"
                     /translation="MSPSKKSKPENKVVTAILRILAVLAVIGITVYVYSIRNHVEEFA
                     NYGYFGAFLIMLIANATVILPAPGVAIVFAMGSMLNPLGVALAAGVGGMLGEMTGYLA
                     GYSGQAAVENTTVYNRIVPYVQKYGAWVILVMSAIPNPFFDLAGIAAGIAKIPVWQFM
                     LACWVGQTIKMSMFAFAGAYSIDWIARLYK"
BASE COUNT         1302 a         1111 c         1172 g         1259 t
ORIGIN      
        1 gcatcataca cagcgcgttt ttccacttcg gtcacaccgg taggtaagcc aacgatcaca
       61 cgcggacgtg gcaaaggcac aacactctgt tcatgtacct ttccaataaa gtactcgagc
      121 atcacacgtg ttacatcgaa ttctgcaatc accccatcac ggatgggacg aaccaccacc
      181 acattggcgg gagtacgccc taccatttcc ttggcttcaa gaccgatcgc aagaggttgg
      241 cgttgtttct tatcaacagc tacccaagac ggttcattga tcacaatacc cttcccacga
      301 acatggacaa gagtatttgc ggtgccaagg tcaatggcaa tatccaaaga aaaaaggcct
      361 aacagccagt taattgggtt gaaggccaaa atcggctcct atgatacgag aatgggtcta
      421 agcaaaactg ccgagccatt ataccatgag gatgagtccg aatatttttt aattctgtta
      481 cccaaacggg gtaaaatcag gaaaattttt ccccgtggag gaagagaaat gtcaaagttt
      541 gccattgtta ccgatagcac ttcttatatc ccaaccgatg ttgcccagaa acatggaatt
      601 accattgccc cacaagttct catttgggac aaccaaacat accgcgacgg tattgatatt
      661 cagcccaccg acttctacag ccgcctcaag accgccaagt cgatgccgtc aacttcgcag
      721 gtttctcctg ccactatgca agagatattt caggggttgg tcgaaaaagg aacttctgtg
      781 cttggcatct tcatctcttc aaaattatca ggcacattgc aatctgccat tcaagccaag
      841 gatatgatgg gaagtgctgg cgaaaaagta accttagttg acagccgctc cactgcaatg
      901 gggcttggtt ttcaggcaat tattgctgct cgtgctgcag aagcaggtgc aagcatcgaa
      961 gaagctgcgg cacaggcgtc tagcgcacat gaacgcacag gggtgttctt tgcggtagat
     1021 acacttgaat tcctgcaccg tggcggtcgt attggaggcg cacaacgctt tattggctct
     1081 gcgctgaacc tcaaaccgat cctcgcagtc aaagaaggca aagtagaagg aatcgaacgc
     1141 attcgcacca agagcaaggc tcatgaacgt gtgcttgaat tggttgccga acaggtcaaa
     1201 ggaaaatcaa acgttcgcat tgcgactctc catgcgaact cggcagacga tgccaaaagc
     1261 ttgttagacc gcgctgcgac tgaactctct ccggtggaaa ccctatttac cgaagttagc
     1321 ccggtggttg gaacccatgc cggacctgga accgtagggc tggcttacat tcacgattaa
     1381 tccatgatcg acccaaagca gtttgaacgt attgttcaat ctatgccttt cttgaagaac
     1441 gcagactcgg gatttgtaaa agaattccaa aagtctgcgt ttcttgcgca gattccggca
     1501 gggcacgatg tctttcttga aggtgatcgt gttgaagcca ctgccttgct gatctctggc
     1561 gtagtacgtg tttataaaat tggtgaaaca gggcgcgaga ttacgctcta tcgctttggt
     1621 aacggttcat cctgtattct gaccgcgaat gcaatcttga gccgaaatac attcccagcg
     1681 attgccacgg ttgagcaaga tgcagaagct gtgatgattc cagccgatgt atttcgagat
     1741 tgggtcaagc gctatgactt gtggcgtgac tttgtctttg acctgttatc tcaacgcctt
     1801 tctacagtga tggctgtagt tgacgaagtt gttttccacc gcatggaccg ccgagtggcg
     1861 aatttactcc tgaagcgcgg aaaggaagaa aaccctattc gcatcacgca tcaggaaatc
     1921 gcttcagatc tgggaagttc cagagaagtg attagccgct tactcgaaga tttcatcagt
     1981 gagggcagcg tccgctcagg gcgtggaatc atcgaagttc tagattttaa gacgttggaa
     2041 gatcgttcgc ttatgtgaca tagtcacaga caggttcggg agtttctcct atactacggt
     2101 taacaataac caaaaggaga aactagaaaa tgaaacgaaa tttgtctgat gtcgaccgaa
     2161 ttatccgcgt ggtgctggct gcaatttttg cctacttgta ctttggtggt atcgttactg
     2221 gaacgttcgg tattgtcttg gtcgtgcttg ctgtcgtatt tgccctcacc gctgtggttg
     2281 cattctgccc gttatatgca cctttcaagc ttagcaccta caaaaagtaa ttcaaaacat
     2341 ttgggagcgg gcaatgtttt gcccgctcct atcctttaaa ttatgaacaa acaagaattt
     2401 caagagaaaa tcaattcaag cgaaaaaccc ctcgtagtgg atttttgggc aacatggtgc
     2461 gctccatgca tgatgaccaa acccattcta gaaaaactcg gcaaagaata tgccgaggat
     2521 gtggaatttc tacccatcaa tgcagatgag tctcgggatg tattggaaga ctatcgtatt
     2581 ctcgggattc ccactgtgct tgcatttaaa ggtggaaaag aagtagcacg agtcactggc
     2641 gctcaaaacg aagcgggcta tcgtgccatc tttgaatcta tggcaaaggg agtgaaagta
     2701 aaaatcccga tgacccaaat tgaccgtatg ctccgtttgg gtgctggcgg tttgctggtc
     2761 atgtatggtt tgcctgacag aaattggatc gttgtagcga ttggcgtggt tgttgctttt
     2821 ttaggtattt atgaccgttg cccaatttgg aatgcactga cgaaaccctt ccagcgaaaa
     2881 taaagttcaa tggttaggaa acaggctccg gcttcgtccg gagccgtttg catcccaatg
     2941 agagtggcgg gtataatctc gtcatatgat gcataaaatc cctttgatta ttggagttgc
     3001 gggcgggtca ggttcgggca aaacaacggt tgcacaggaa ttattacgaa gagtgggggc
     3061 agatcgaatt gcttatcttc aacacgattc atattacaaa gatttgcgaa tgttgccatc
     3121 tactcaacat tctgaaatca attttgatca tccagacacg cttgagactg agttattaat
     3181 caaacatatt attgacttgc gcgagttcaa agccgtgcag attccgattt atgatttttc
     3241 aacggacaca cgtaagacag agacaatcac tgtagaaccc cgaaatgtga ttttagtgga
     3301 agggattctt ttgtatgtgg agcctgagct acggaagctt tttgatgtaa aactttttgt
     3361 agatgcggat gcagacattc gcttcattcg ccgcctgcac agagacatca ccgaacgcgg
     3421 acgatccact gaatctgtga tcgaacaata ttatgcaact gtgcgcccaa tgcatttgga
     3481 atttgtagaa ccttcaaagc gctatgcaga tgtcattatc ccagagggcg ggcataacgt
     3541 tgccgctctg gatatggtta cagcaagaat cgaattgctg ctcaaataaa cccaatttta
     3601 tattcatcta ctcaaggaga gacgtatggc aaaacaatgg agcacccccc cggcaatgca
     3661 gatcgacccc aagaaaaaat acaaagctca catggaaaca gataacggta cgatggtgat
     3721 cgaactgttc gctgataagg cgcctgtaac cgtgaataac tttgtgtttc tttcgcgcga
     3781 aggctattat gacggtgtga tctttcaccg tgtgatctcg aactttatgg cacaaggcgg
     3841 cgacccgaca ggtaccggac gcggcggacc cggctataaa tttcaggatg aattccaccc
     3901 aagcctgaag catagcaagc gcggcatcct ttccatggca aatgcaggac ccggcacaaa
     3961 cggctcgcaa ttcttcatta ctcacggacc caccccgcac ttggataacc gtcacaccgt
     4021 gttcggtgaa gtggtggaag gcgaagatgt gttgatgtcc attcctgaac gcgacccgat
     4081 gaagctcaac gccccggctg taaagatcat ccgtgtcacc atcgaagaaa tctaagcctg
     4141 aaaataaagt ggtcaccgcc atactgcgca ttctggcagt actggcggtg attggcatta
     4201 cggtctatgt gtacagtatc cgtaatcacg tagaggaatt tgccaattat ggttattttg
     4261 gcgcgttcct catcatgttg attgcaaatg cgaccgtgat tctaccggct ccgggtgtgg
     4321 cgattgtttt cgccatgggc agtatgctga atccacttgg ggttgcactg gctgcagggg
     4381 tcggcggtat gttaggtgaa atgacaggct atttggcagg ctatagcggg caagccgctg
     4441 tagaaaatac aacggtctac aaccgcattg tgccatatgt gcaaaagtat ggtgcctggg
     4501 tcattctcgt tatgtcggca attccaaatc cattttttga cttggctggc attgcagcag
     4561 gcattgcgaa aattcccgtc tggcagttta tgttggcatg ctgggttggg caaacaatca
     4621 aaatgtctat gtttgctttt gccggagcct attccattga ctggatcgcc cgtttatata
     4681 aataaccaaa acctgacagg tgctctgtca ggtttgtttt tatctcagca aaggagtttc
     4741 agatgagcaa ttatgacaaa gtcgatgcct tcatcgataa aaatctaagc cagagtttag
     4801 atgaactcaa aaagtatgtt gcccaaccaa gtatcagcgc tcaa
//
LOCUS       DAOREF010000096         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9984_length_4833_cov_5.021348, whole genome shotgun sequence.
ACCESSION   DAOREF010000096 DAOREF010000000
VERSION     DAOREF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9984_length_4833_cov_5.021348"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1043
                     /locus_tag="PKI33_02985"
     CDS             180..1043
                     /locus_tag="PKI33_02985"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNM35995.1"
                     /translation="MSSPFIPKRIVVTAYPKMPEAFTEAAAMSVFLKEKGIDAPVGSL
                     YDEELRKRVKQGEFDMLIAVGGDGSVLRAGHLCAPSGVPILGVNLGRLGFLIQIDRKE
                     WRDYFMRLLAGEAWIENRMMLRAEHIRAGEKLGSSLALNEVVVGRGQNLRPVRLTAYV
                     DKRELTSYVADGLIAATATGSTAYALAAGGPILPPELRNILLVPIAPHLSVDRAVVLS
                     EGTSVDIVMKSENAIFSVDGQPPTPLMEEDHVNVTATDVTAQFVRFGNPGYFYRNITM
                     HINENSSGFPR"
     gene            1040..1564
                     /locus_tag="PKI33_02990"
     CDS             1040..1564
                     /locus_tag="PKI33_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM35996.1"
                     /translation="MTEFSTTTTCYVHPNRETSLRCKRCERYICTSCAVSTPTGYICK
                     ECMRERQKSFDTALWYDFALGFVVAGLLSGVASFLVTLIGAIGFIGFMLVFIGSSAAG
                     GIIAEAVRAVTRRHRSRALFMTVGAGVIAGALPMLLVQLLSVNVFGLIFQGIYLVIAV
                     PLVYARLSGIQLTR"
     gene            1568..3304
                     /gene="recN"
                     /locus_tag="PKI33_02995"
     CDS             1568..3304
                     /gene="recN"
                     /locus_tag="PKI33_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNM35997.1"
                     /translation="MLTELHIQNFAIIDKLDLRFGPGLIILTGETGAGKSIILDAVVM
                     LIGGKADTTFVRSESDAAFVEGVFQLKGPEREAVHEVLNREELMDDPDYVVLMREVRK
                     EGRSVARINGRTVNVGLLKELGALLIDIHGQAEHLSLLDPRAHLGLLDRYADVAKPLN
                     DYRQTYHSLLTLRHELNDLRKAQADSDRRIEMLTYQVEEIESAKLKVGEDEELRKERD
                     RLANAESLAQNAQEALAILEEGSPETPAATDLIGQAAQALTALAKIDAGQSELANQAE
                     VMLDTLSDIIHEVRNYLEEIEFNPKRLDDVEERLDLIHSLTRKYGGNIPAVISYGADA
                     KKQLETITGAADRIAELEMQEAKFLEKLSKQGNILSEKRKSAAETMSKGIELELDDLR
                     MASAQFGVDFQTKADPNGIPIADGTRLAFDQNGIDRIEFLVAPNPGEGLKPLAKVASG
                     GETSRLMLGLKNVMARADEVPSLIFDEIDQGIGGRVGMVVGHKLWNLSRSHQVFCVTH
                     LPQLAVFGDQHYQVQKLVDKGRTLTRVEPLEGESRLLELSQMLGEVGEGTLRSAHELL
                     QTARQMVKESKK"
     gene            3494..3820
                     /locus_tag="PKI33_03000"
     CDS             3494..3820
                     /locus_tag="PKI33_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNM35998.1"
                     /translation="MQEDLTEMLKGVLEGCILEIISRKKTYGYEITRQLNTLGFTDVV
                     DGTVYTILVRLEKNKLVDIEKSPSEMGPPRKFFTLNNAGREELRKFWEKWKFISSRID
                     KLQEKS"
     gene            3820..4167
                     /locus_tag="PKI33_03005"
     CDS             3820..4167
                     /locus_tag="PKI33_03005"
                     /inference="COORDINATES: protein motif:HMM:NF018057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HNM35999.1"
                     /translation="MNFWEKITGNDLKQEFKTFESRVQKLPADYQAAWEEINGTLWLH
                     SDFTGHNIMSILSGVLDLFEETSADGQSVQETLGDDIKGFCSALASEAGAKSYRDKWR
                     DQLNNTIAKKLGG"
     gene            4177..4527
                     /locus_tag="PKI33_03010"
     CDS             4177..4527
                     /locus_tag="PKI33_03010"
                     /inference="COORDINATES: protein motif:HMM:NF018057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HNM36000.1"
                     /translation="MSIKDIIEGKRQWRAHMSRVNALPQDYRIVYKEIQKYLFKVGIV
                     ELTEGTNILSGIVDLFTEGAAMQKGVLEVTGNDVAAFCDNLLKNSKTYSDVYQEAVDQ
                     EVKKAMKIAMTKTK"
BASE COUNT         1323 a         1167 c         1174 g         1169 t
ORIGIN      
        1 tgaactggaa acagcgctga ttgaactcat tgccgatatg cgcgaagacg acgtccgcag
       61 agatgtggct cggtcgcatg agcaggcgtc ccgttgtaag cgatgcgggt atcatgatat
      121 ttgtgaacaa cggctggcat aacatccgtt agcaaagaca aaaggttata atttccggca
      181 tgtcttcgcc tttcatccca aagcgtatcg tcgtcacggc gtatcctaaa atgccggaag
      241 ccttcaccga agcagcagcc atgtctgttt ttctcaaaga aaaaggcata gatgctcctg
      301 tcggttcgtt gtacgatgaa gaactacgca agcgggtgaa acaaggtgag tttgatatgc
      361 tgatcgccgt tggcggcgat ggcagcgtct tgcgagccgg gcatttgtgt gccccatcgg
      421 gcgttccgat cttgggagtg aatcttggca ggctcgggtt cctcattcag atcgaccgca
      481 aagaatggcg cgattatttt atgcggttgc tcgctggcga agcatggatc gaaaatcgta
      541 tgatgttgcg cgctgaacac atccgtgctg gtgagaaact tggttcgtca ctggcgttga
      601 atgaagtcgt cgtcgggcgc ggacaaaacc tccgcccggt gcgtttgacc gcttatgtcg
      661 ataaacgcga gctcaccagt tatgtcgccg atggtttaat tgcagcaacg gcaaccggct
      721 ccacagccta tgccctcgcc gcaggcggac cgatcttgcc gcccgaattg cgcaatattt
      781 tattggttcc cattgcgccg catctttctg tagaccgagc tgtcgttctt tcagaaggca
      841 cgtctgtaga tatcgtaatg aaaagtgaga acgccatttt cagtgtggat ggtcagccac
      901 ccacaccctt aatggaagaa gaccatgtca acgtcacggc gaccgatgta accgcacaat
      961 ttgtacgatt tggaaaccca ggatactttt acagaaatat caccatgcac ataaacgaaa
     1021 attcttcagg atttccacga tgaccgaatt ctccaccaca actacttgtt atgttcaccc
     1081 gaacagagaa acctctctgc gttgcaaacg ctgtgagcgt tacatctgca cctcatgtgc
     1141 cgtcagcacc cctacgggct acatttgcaa agagtgcatg cgcgaaaggc aaaagtcttt
     1201 cgacaccgct ctttggtatg attttgcctt gggctttgtc gttgccggac tcctctctgg
     1261 cgttgcatcc ttcttagtga ccttgatcgg tgcgattggc ttcatcggtt tcatgcttgt
     1321 tttcatcggc tcttccgccg ccggaggcat aatcgctgaa gccgttcgag ctgtcactcg
     1381 ccgacaccgc tcgcgcgcgc ttttcatgac agttggcgct ggtgtgattg cgggtgcctt
     1441 gcccatgctt cttgtacaac ttttgagcgt caatgtcttt gggttgatct tccaaggcat
     1501 ttatcttgtt attgctgttc ctttagtcta cgcccgcctt tccggtattc aactcactag
     1561 ataacctatg ctcacagaac tccacatcca aaattttgcg atcatcgaca aactcgacct
     1621 gcgttttggt ccgggtttga tcattctcac cggtgaaacc ggcgcgggca aatccatcat
     1681 ccttgatgct gtggtcatgc tcattggcgg caaagccgac acgaccttcg tgcgctctga
     1741 atcagacgcc gcatttgttg aaggcgtgtt ccagctcaaa ggtccagaac gtgaggcggt
     1801 gcatgaagtc ctcaaccgcg aagaattgat ggatgatccc gattatgttg tgctcatgcg
     1861 tgaagtccgc aaagaaggac gcagcgtggc acgcatcaac gggcggacgg tgaatgtagg
     1921 cttgctcaaa gaattgggcg cgctcctgat cgatattcat gggcaagcag aacatctctc
     1981 cctgctcgac ccacgcgcgc atcttggctt gctcgaccgc tatgccgatg tagccaagcc
     2041 attgaacgac tatcgccaaa cctatcactc gttgttgacc ctgcgccatg agctgaatga
     2101 cctgcgcaaa gcccaagccg actcagaccg ccgcattgaa atgctcacct atcaggtaga
     2161 ggaaattgaa tctgccaagc tcaaagtggg cgaagatgaa gaactgcgca aagaacgaga
     2221 ccgtcttgcc aacgcagaat cactggcaca gaatgcccaa gaagcgttag ccattcttga
     2281 agaaggctca cccgagactc ccgccgcaac cgatctgatt gggcaagccg ctcaggcatt
     2341 gaccgcgctc gcaaagatcg acgcgggtca atctgaattg gcaaatcaag ccgaagtgat
     2401 gctcgatacg ctttcagaca ttattcatga agtgcgtaat tatttagaag agatcgaatt
     2461 caaccccaag cgtttggatg atgtcgaaga acgcctggat ctcatccact ctctgacccg
     2521 caagtatggc ggcaatattc cggctgtcat ctcatacggg gcagacgcaa agaaacaact
     2581 tgaaaccatt accggcgcag cagaccgcat tgccgagctt gaaatgcaag aagccaagtt
     2641 ccttgagaaa ctttccaagc aaggcaatat cctttctgaa aaacgcaagt ctgcggctga
     2701 aaccatgagc aaaggcattg aactggagtt ggatgatctg cgcatggcat ctgctcaatt
     2761 cggcgtggac ttccaaacca aggcagatcc caatggtatt cctattgcag atggaacccg
     2821 cctcgccttc gaccagaacg gtatcgaccg catagaattt ctcgttgccc ccaaccccgg
     2881 cgaagggctt aagccgttgg caaaagttgc ctctggtggt gaaacctcac gccttatgct
     2941 gggtctgaaa aacgtcatgg ctcgcgccga tgaagtgcct tctctcatct ttgatgaaat
     3001 cgatcagggc atcggcggac gtgtaggtat ggtcgtcggt cacaaactgt ggaatctatc
     3061 gcgcagtcat caagtcttct gcgtgactca tctccctcaa cttgctgtct tcggtgacca
     3121 gcattatcaa gtccaaaaac ttgttgataa aggtcgcaca ctcacccgtg tagagccgct
     3181 tgaaggtgag tctcgtttgc tggagctatc gcaaatgctc ggcgaagtcg gtgaagggac
     3241 gttacggtcc gcacacgagc tcttacaaac cgcaagacaa atggtaaaag aaagcaaaaa
     3301 ataaaaaatc gggcgatgac tatcgcccga ttttttattc aggcatcccc taaaatcatt
     3361 gttttcttga agagcctgtt tttgcaggcg gcataagact agctaaattt aagaataaaa
     3421 gctattgaca gcctagctac tacgtgctac tatatagcag tagtaagtaa tactatatag
     3481 actggaggca aatatgcagg aagacctaac agaaatgctc aaaggtgtgc tggaaggctg
     3541 catccttgaa atcataagcc gcaaaaagac ctacggctac gaaatcacaa ggcaactaaa
     3601 caccctcggt ttcactgatg ttgttgatgg aacggtatat acgattctag tgcggcttga
     3661 aaaaaataaa ctggtggaca tagaaaaaag cccatctgaa atgggtccgc cacgcaagtt
     3721 tttcacactc aacaatgccg gtcgcgaaga actacgaaag ttttgggaaa aatggaagtt
     3781 tatatcgtca agaattgaca agctacagga gaaaagctaa tgaatttttg ggaaaagatc
     3841 acaggcaacg acttgaaaca agaatttaaa acgtttgagt cgcgagtcca aaaattacca
     3901 gcagattatc aagcagcatg ggaagaaatt aatggcaccc tttggctgca ttcagatttc
     3961 accggtcaca acatcatgtc gattcttagc ggtgtacttg acttattcga agaaacatca
     4021 gcagacggtc aaagcgttca agagactttg ggtgatgata tcaaaggctt ctgttcagca
     4081 ttggcaagtg aagcaggggc aaagtcttat cgtgataagt ggcgcgatca acttaacaat
     4141 accatcgcca aaaaattagg cggctaggag aatataatga gtataaaaga cattatcgaa
     4201 gggaaaaggc agtggcgagc acacatgtca cgtgtcaatg cgctcccaca agattatcga
     4261 attgtttata aagagattca aaaatacctc tttaaggtag ggattgttga gctgaccgaa
     4321 gggacaaata tcctctcagg cattgttgat ctttttacag agggtgcagc aatgcagaaa
     4381 ggcgtgcttg aagtaaccgg caacgatgta gccgctttct gcgataatct gctcaaaaat
     4441 tctaaaacgt actcagacgt ctatcaagaa gctgtggacc aggaggttaa aaaagccatg
     4501 aaaatcgcta tgaccaaaac aaagtaaaag gcgaatacga tttagccaat tgttcactca
     4561 accgcaacat gaggacctat aaaatctaca gacaaaaaac aggcgctttt gcaaaagcgc
     4621 ctgttttttg tctgagtctg gaaattaaaa tgttgacagc cagggtgagg ggggcaccct
     4681 ggctgtcaac ggttgtaata atacacccag catttccaaa agtcaagtac ttcttgctga
     4741 gaaaaaaatt agaggtcttt tactagtata aacaactagt gccctgagta ggaatcgaac
     4801 ctacatctaa gccttaggag tgccttgttc tat
//
LOCUS       DAOREF010000097         4828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_9999_length_4828_cov_5.196313, whole genome shotgun sequence.
ACCESSION   DAOREF010000097 DAOREF010000000
VERSION     DAOREF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9999_length_4828_cov_5.196313"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2104
                     /locus_tag="PKI33_03015"
     CDS             <1..2104
                     /locus_tag="PKI33_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36001.1"
                     /translation="LSVKVYDNVHAYGILLVPSRVPSWLDGFPWNTPLEFITTTLALP
                     FAFFFGRKFLSKKAVAVLLTLLLAVKLTLSLFLPQSGLGIRTYFSEAELSSNTWARTY
                     ALNPSFSEVARLPYRSFLDFPVEFINTHGFEKNSFWMTMKFNGFISLGEDERLVILLQ
                     GAEQRQIELVELATGEVYEVATAKSVDDLGKATFGELSFVGNAELHGSLLFTNYGNGR
                     FEPVILSSDGSVRSAYSQIWLAAPSLDFSSDVFQFIQKLMDMLFLGCILWSLWDGLSA
                     LYRLGMIDMVDTYLAMTGLGLYYIVDTAYKPTVHFLILPIILALILVKLLDIVLRSRP
                     YSAKGYFFSLGFPILLIFLALDLPALQSVVVLPQYQDVSDYQILARNIYVAGDTFLFR
                     SPPWAYKVLYPYVIGLLHILFGQSLSAQLFLNVWSALLSVGLTIEFAVFFGLSKRLSF
                     ISASIFLLILLLPSSFIYFFRFGLIEPLAIMTLLLGAYFAREGKFNAMFITGLVTGML
                     RLNFAGAIFTSVTFLGSAFLGGFSQAWGSFIHGLRLSWKRWVFYLAAIPLPSLLIALV
                     YTRVRPGYTLTHEMNDQTSIGSVLLSITRVVFGGDEEFLQNQIRNNPMDLVLITLPIL
                     FGLGIALISLVYRKGIFEKVDLRLSLFLLSMLPVYVVLKPIGYFPRYSWSFLPPALVL
                     LGLVLQLTVLRDNKALQD"
     gene            2107..3474
                     /locus_tag="PKI33_03020"
     CDS             2107..3474
                     /locus_tag="PKI33_03020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNM36002.1"
                     /translation="MRSSFQNTFRIWVVVTFLVLASLLAIYLRNGMVGLNLFIQEWPL
                     SNLAVTLASTSLTWILAGALLYLLANEKINKGNLWLTAGFLLVMFVYLQILRERLRYG
                     DYHYYLEAATALANGQPLPDTYLYLPLWGTLLRFLVPLGDQGVMIALWTVNVIALGSF
                     YLLLVSVLQRYNFSQHLSIVVAILFLLINTPLMRTLGFIQVNLMTIDLILLCILVYPK
                     NNFLSALALAFAVHLKTSPAVIVLAFLLEFNWRWIFWFAISFLAIGLFPVAMHGVDIY
                     LQFVNNTIGLAQIPDTNFHDTSFDSFLRFLNPFFGIQIETTRLMALGAKVLLLLAALY
                     TMAQNIFAQSFSKENRLLNAAPALFVFMTLASPIVWDHHGIFTTLAFLLMLKRIQSPS
                     HWMIFLAAYFFEFMLPSFDFFPWSYGRLIAPIVILWLMYATRKNDEPSPFIASANQWL
                     AKLAS"
     gene            3478..>4828
                     /locus_tag="PKI33_03025"
     CDS             3478..>4828
                     /locus_tag="PKI33_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36003.1"
                     /translation="MKKHLSTIILACVFIAGLLTFDHYGESWDDRSLQKYAEKSIAAY
                     TTFPQEGVVNIVREDLGFYGPFFVMLTDVLSKSLSTFLPFSLPDLRHLIYYLTYFAGV
                     LAFHSIAKRWLTQIPALGATLLFALQPVFWGHAFMNPKDTPFLALFLLSISLGMQAFD
                     SLKLDSRIDLSLNSKRTLTLLTVLWLVSIFGLFIFTQAIHTYIEVLVHSAQAGNTNLF
                     SLVAKDIAAVPAETYTQRYFILFVQLRTYYSLLITLILLIAYYRLNPNLPNNIFTILP
                     AALLLGLSTSTRILGPFAGVIIAYYALRTKGKQSIPAILIYAILALIATYISWPYLWM
                     NPIPRFFESLKEMSLYPWLGGVIFNGDLYQSTNLPASYLPTLLVLQLTEPVWLLSLAG
                     WGVAIQDKEKKRTLFEVALLWFVIPLLAFIVLKVALYDNFRQILFILPPIFLMAGVAF
                     "
BASE COUNT          956 a         1343 c         1010 g         1519 t
ORIGIN      
        1 cctgtccgta aaggtgtacg acaacgtcca tgcctatggc atcctgctcg ttccgtcccg
       61 tgtcccatca tggttggatg gcttcccatg gaacacccct cttgaattca tcaccacgac
      121 cctggccttg ccattcgcat ttttcttcgg ccggaaattc ctctccaaga aagcagtggc
      181 ggttcttctt acgctcttgc ttgctgtcaa actgaccctg agtctttttc tcccccaatc
      241 cggtttgggt atccgtactt actttagtga agcggaactt tcatccaata catgggcacg
      301 gacatatgcg ttaaatccat cattcagcga agttgcgcgt ctgccatatc gctcatttct
      361 tgattttccc gttgaattca tcaacaccca tggcttcgaa aagaactcct tctggatgac
      421 aatgaagttc aatggattta tttcgcttgg cgaagatgaa cgtctggtca tcctcctgca
      481 aggcgcagaa cagcgtcaga tcgagttggt cgaacttgcc accggtgaag tctatgaagt
      541 tgctaccgcc aaatctgtag atgatctggg taaagccacc ttcggtgaat tgtccttcgt
      601 gggaaatgct gagttgcacg gctcgcttct tttcaccaat tacgggaatg gacgttttga
      661 acccgtgata ttaagttcgg acggatcggt ccgttcggca tattcacaga tctggcttgc
      721 cgcaccctcg cttgattttt catctgatgt gtttcagttc atccagaaat tgatggatat
      781 gctcttccta ggatgtatct tatggagctt gtgggatgga ttatccgctt tgtatcgttt
      841 ggggatgatc gatatggtgg atacctacct cgcaatgacg gggttgggtc tatattacat
      901 tgtggacact gcttataagc ccacagtcca ctttttgatt ttgccgatca ttcttgcgct
      961 tatccttgtc aaactgttgg atattgtcct gcggtcgaga ccctattctg caaagggata
     1021 ttttttctca ttgggtttcc cgatcctgct catattcctg gcgctggatt tgcctgctct
     1081 gcaaagtgtg gtggtattgc cgcaatatca ggatgtttca gattatcaaa ttttggcgcg
     1141 taacatctac gtcgcagggg atactttcct tttcaggtcc ccgccctggg cttacaaggt
     1201 cctttacccc tatgtgatcg gacttttgca tattttgttt ggccagtcgc tttcggctca
     1261 actctttctc aatgtctggt ctgcgctctt atcggttgga ctgaccattg aattcgccgt
     1321 tttctttggc ttgtcaaaac gcttgtcatt tatttcagcc agtatctttt tattgatcct
     1381 gttattgcca tccagtttta tttatttttt tcgctttggt ttgatcgaac cccttgcgat
     1441 catgaccctg ctgcttggcg cctactttgc cagggaggga aaatttaatg cgatgttcat
     1501 caccggtttg gtgaccggaa tgttgcggct taattttgca ggtgctattt ttacttcggt
     1561 cacctttctt ggatcggctt tccttggcgg gttctcccaa gcctggggat ctttcatcca
     1621 cgggctgcgg ttaagttgga aacgttgggt gttctatctt gctgccatcc cgctgccgtc
     1681 gctgctgatc gctttggtct acacccgtgt caggcctggt tatacgttga cccatgaaat
     1741 gaacgatcag acttcgattg ggtctgtgtt gttaagtatt acaagagtgg tgttcggtgg
     1801 cgacgaagaa tttctgcaaa atcaaattcg gaataatcca atggatctgg ttttgatcac
     1861 gctcccgatc ttgtttggtc tgggcatcgc attgatctcg cttgtgtatc gcaaaggcat
     1921 ctttgaaaag gttgacctgc gcttaagcct gttcttgttg agtatgctgc ctgtatatgt
     1981 ggtgctgaaa ccgattggat atttcccgcg ctacagttgg tctttcctgc cgcctgccct
     2041 ggttctactc ggactcgttt tgcaattgac tgttttaaga gataataagg cgcttcagga
     2101 ttaacaatga gatcatcttt tcaaaataca tttcgcattt gggttgtagt aacttttctg
     2161 gttcttgcca gcctgcttgc catctacctc cgcaatggca tggtgggact taacctcttt
     2221 attcaagagt ggccgctttc caaccttgcg gtgactctcg cttcgacctc cctcacatgg
     2281 atcttggcag gcgcattgct ttatctgctt gccaatgaaa aaataaacaa aggcaatctc
     2341 tggctcacgg cgggattctt actcgtcatg ttcgtgtact tgcagatttt gcgcgaacgc
     2401 ctccgctatg gcgactatca ctactacctc gaagccgcca ccgccctcgc caacggacaa
     2461 cccctgcccg acacctatct ctatctgccg ctttggggta cgttgttacg attcctcgtc
     2521 ccgcttggcg atcagggagt gatgatcgcc ctctggaccg tcaacgtcat tgcgctcggt
     2581 tctttttact tattgctcgt ttctgttctt caacgatata acttctcaca gcatctttct
     2641 attgttgttg ccatcctatt cttgctcata aatactccgc tgatgcgtac acttggcttt
     2701 atccaagtca atttgatgac gattgacttg atattgctct gtattctggt ttaccctaaa
     2761 aataattttc tatctgcttt ggcacttgct tttgcagttc acctcaaaac ctcaccggcg
     2821 gttatcgtgc tggcattcct gcttgaattc aattggcgct ggatattctg gtttgctata
     2881 agttttcttg ctattggtct tttccctgtc gccatgcatg gagtggacat ttacctgcag
     2941 ttcgtgaaca atacgatcgg gctcgcccag attcccgata ccaatttcca cgacacgtcc
     3001 tttgattctt tcctgcgttt cctcaatccc ttctttggca tccagatcga aaccactcgt
     3061 ctgatggcac tcggcgcaaa agtgcttttg cttctcgccg cgctttacac catggcgcag
     3121 aacattttcg cgcagtcttt ctccaaagag aatcgcttac tcaacgccgc acccgccttg
     3181 ttcgtgttca tgacgctggc ttcccccatc gtctgggacc atcatggcat tttcaccaca
     3241 cttgctttct tgctgatgct aaaacgcatc caatctccat cgcattggat gatcttcctc
     3301 gccgcctact tcttcgagtt catgctgccc tccttcgact tcttcccttg gtcgtatggt
     3361 cgccttatcg cgccgatagt cattctctgg ttaatgtatg ccacccgcaa gaacgacgag
     3421 ccttcaccct ttatcgcgtc tgcgaatcaa tggctggcaa aacttgcaag ttagcccatg
     3481 aaaaaacatc tctctacgat catcctcgcc tgtgtcttca ttgcaggctt actcaccttc
     3541 gaccactatg gcgaaagctg ggatgaccgt tcgcttcaaa aatacgccga gaaatccatc
     3601 gccgcttaca ccaccttccc gcaagagggc gtggtcaaca ttgtgcgcga agacctcggc
     3661 ttttacggtc ccttcttcgt catgctcacc gacgtcctct cgaaatcgct gagcaccttc
     3721 ctgcccttct cactccccga cctgcgtcac ctcatttact acctcaccta tttcgcgggc
     3781 gtgctcgcct ttcactccat cgccaaacgc tggcttaccc aaatacccgc cctcggcgca
     3841 acgctactat tcgccctgca acccgtcttc tggggtcacg ccttcatgaa cccaaaagac
     3901 acgcccttcc tcgccctctt tcttctttcc atctctttgg ggatgcaagc ctttgactcg
     3961 ctcaaacttg attcccgcat cgacctttcc ctcaactcca agcggactct cacgcttctg
     4021 acagtgctct ggcttgtttc tatcttcggg cttttcattt tcacccaagc gattcatacc
     4081 tacattgaag tgcttgtcca ttccgcacag gctggtaata ccaatctctt ctcgctcgtt
     4141 gccaaagaca ttgctgctgt tcccgctgaa acctataccc aacgttactt catcctcttt
     4201 gtacaactcc gcacttatta ctcgttactc attactctta ttcttctcat tgcctactac
     4261 agactaaacc ctaatctacc aaacaatata tttaccatcc tacccgccgc cctcctgctg
     4321 ggactctcca cctccacccg catcctcggt ccattcgcag gtgtcatcat tgcatattac
     4381 gcattgcgca ccaaaggcaa acaatccatc cctgcaatct tgatctacgc catactcgca
     4441 ctcatcgcca cctacatctc ctggccctac ttgtggatga atccgattcc aagatttttc
     4501 gagagcctga aagaaatgtc cttgtacccc tggctgggcg gagtcatctt caatggtgat
     4561 ctttaccaat ctaccaatct accagcctcc tatctaccaa cattacttgt ccttcagttg
     4621 accgaaccgg tctggttgtt gtctttggct ggatggggag tggcaattca agacaaggaa
     4681 aagaagcgta cgctcttcga agtcgccctg ctgtggtttg tcatcccgct gctggcgttc
     4741 atagtgttga aggtcgcgtt gtacgataac ttccggcaaa ttttgtttat cctgccgccg
     4801 atctttttga tggcaggtgt tgcctttg
//
LOCUS       DAOREF010000098         4818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10039_length_4818_cov_4.032333, whole genome shotgun sequence.
ACCESSION   DAOREF010000098 DAOREF010000000
VERSION     DAOREF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10039_length_4818_cov_4.032333"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKI33_03030"
     CDS             complement(<1..316)
                     /locus_tag="PKI33_03030"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNM36004.1"
                     /translation="MILTDSRERTRFFKFAFVGAIGAAIDFGVMNLLSHVILELPLVF
                     AGSISFICAVISNFTWNRYWTYPESRSRPLLSQLGMFFAVNTAGMLIRIPILHYLEPP
                     LLQ"
     gene            complement(313..1272)
                     /locus_tag="PKI33_03035"
     CDS             complement(313..1272)
                     /locus_tag="PKI33_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM36005.1"
                     /translation="MRILITGAAGFLGSHLCDRLLADGHEIIGMDNFITGNPDNIAHL
                     AGNEKFQFFKRDVSNYIFVPGKVDAILHFASPASPNPQSKSGYFNLPIQTMKAGALGT
                     HNCLGIARREGSRFLLASTSEIYGDPEIHPQAETYPGNVDPVGERAVYDEAKRFAESM
                     TMAYHRFHSVNTSIVRIFNTYGPRMDLEDGRALPNLLKQALLGEPLTVYGTGSQTRSF
                     CFVDDLVDGIVKLLYSKEHLPVNIGNPVEMSILEFAEIINKVTGNTAGIKYIENARSA
                     RDPQRRRPDITRAKDILNWEPKVNLEEGIRRTIPFFKQKLGLA"
     gene            complement(1296..2885)
                     /locus_tag="PKI33_03040"
     CDS             complement(1296..2885)
                     /locus_tag="PKI33_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNM36006.1"
                     /translation="MPRLTLRPSLLATILLLVTLLASCKSPQLNSDITITVTADGTTR
                     DITVPQGQTVSQALESAGITVNSLDKTEPPLYTVIGNGDLVTLIRVEEVFETEEIVVP
                     FERQVVRNETLPEGETRLVQAGVNGLEEITYRKILEDKVETSQSIVKTTVIKEALPEI
                     VMVGAQTSFVPLNIPGTLAYLAGGNAWIMEGSTANRRVIVSTGDLDGRIFTLSPNGEY
                     LVFTRKSSKPADEEINTLWAVRTRNLDPKLISLQAKNVVHFAAWIPGTNSIAYSTVEP
                     RSAAPGWQANNDLYRVSITNSGSPKRILYPSSGGVYGWWGMNFAFAPDGRLAYSRPDG
                     IGLVDQDSGRLKPLLDITPLNTHSDWAWIPPLVWGADGNGLYFVSHAPAPAPISGEES
                     PYFDLSAISFKNEAAVSLVNSAGMFSYPAISPIQYGSREAAFQIAYLQSIFIDQSDSS
                     RYRLVVMDRDGSNSRALFPPADKNGLEPQTPVWAPAAVEGQSGEFIAVMYQGNLWLID
                     SGNSNTYQVTGDGLITRLDWK"
     gene            complement(2905..3474)
                     /locus_tag="PKI33_03045"
     CDS             complement(2905..3474)
                     /locus_tag="PKI33_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36007.1"
                     /translation="MKTKNYIYLFIVSLVSASLACSINVGGPEYPAQPISASPTDAQA
                     LRDMIQQAMVTGAETGVITLSVTESQLTGYMAEKLSQQTNPPFTDPQILLRNGQMQMY
                     GKVTQGWFTANMLITMNVTIDETTGQPKIQITSADFGPIPAPEGVNNAISAIIEEAFT
                     GSFGPVAVGFRLQTITIADGIMPLTGRIK"
     gene            3519..4088
                     /gene="scpB"
                     /locus_tag="PKI33_03050"
     CDS             3519..4088
                     /gene="scpB"
                     /locus_tag="PKI33_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNM36008.1"
                     /translation="MSESEPITNEELSLAAKIEAMLFVSADPVPVQQLSQALDVTASV
                     VERGLKELDEALKSRGLRLQRNAGRVQLTTAPEIAGLVETFLGLEAITHLSRAALETL
                     AIIAYQQPVTRPQIDSIRGVNSDGMMKSLLSKGLIQESGRTEGPGRPILYSTTPEFLQ
                     HFGLSSIMELPPLATPIETEPEPQELLKG"
     gene            4103..>4818
                     /locus_tag="PKI33_03055"
     CDS             4103..>4818
                     /locus_tag="PKI33_03055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNM36009.1"
                     /translation="MQKILAQAGYGSRRACEEFIIEGRVSVNGQIAILGQKADLAVDT
                     IKVDGKSLSKPETMTYIALYKPRNVLSAAEGQDDRKTVRDLIPAPGHLYPVGRLDFDS
                     EGLILMTNDGDLTNKLTHPRYAHEKEYRVLLACKPDTEQLDAWRRGVVLPEGDRTQPA
                     NVHFISTSGKGAWIRVVMGEGKKRQIREVGKLLGLPVVRIIRVRIGTLKLGDLKPRQW
                     RYLTEDEIRELKGDKVPKME"
BASE COUNT         1231 a         1086 c         1271 g         1230 t
ORIGIN      
        1 attgcagcaa cggcggttcg agataatgaa ggatgggaat ccgaatcaac atgccagctg
       61 tattgactgc aaagaacatt cccaactggc tcagaagcgg acgagagcgt gattcgggat
      121 acgtccaata acggttccaa gtgaagttac tgatcactgc acagatgaaa gagatgctcc
      181 ctgcaaatac caacggcaat tcaagaatga cgtgtgaaag cagattcatc acgccaaaat
      241 cgatggcggc gccaatggca cctacgaacg cgaatttgaa aaagcgcgtt cgttctcttg
      301 agtctgtcag gatcatgcca gccccaattt ttgtttgaag aatggaatcg tgcgccggat
      361 gccttcctct aaattgactt tcggttccca attcaagatg tcttttgcac gggtgatatc
      421 agggcggcga cgttgtgggt cgcgagcact acgggcattt tcaatgtact taataccagc
      481 tgtgttgccg gtcactttgt tgatgatctc tgcaaattcc aaaatggaca tttctaccgg
      541 gttgccaatg ttgaccggta aatgttcctt ggaatacaac aactttacga tgccgtccac
      601 caaatcgtca acgaagcaga aagaacgggt ttggctgcca gttccataca cggtaagagg
      661 ctctcctaac aatgcctgtt tcagcaagtt aggcaaggca cgcccatctt ccaaatccat
      721 gcgtggaccg tacgtattaa agatgcgcac aatgctggtg ttcacactat ggaaacggtg
      781 atacgccatg gtcatggatt ctgcaaaacg cttggcttca tcatagacgg cgcgctcacc
      841 gaccgggtct acattaccag gatatgtttc tgcctgcggg tgaatttcag gatcaccata
      901 aatttcactg gtagaggcaa gcaagaagcg cgagccttca cgccttgcaa tgcccaagca
      961 gttatgggtg cctaaagcgc cagccttcat ggtttgaatg ggtaaattga aataaccaga
     1021 ttttgattgt gggttggggc tggcaggaga ggcaaaatgc agaatggcat ctactttgcc
     1081 aggaacgaaa atatagtttg atacatcacg tttaaaaaat tgaaactttt cattccccgc
     1141 caagtgggca atattatctg ggttgcccgt aataaaatta tccatgccaa tgatctcgtg
     1201 accatctgct agtaagcggt cgcagagatg agagccaaga aaccctgccg cacctgtgat
     1261 caaaattcgc attatgttac acctcttctt tttatttact tccagtccaa ccgggtgatc
     1321 aatccatcac cggtgacttg ataggtattg ctattgccac tgtcgataag ccaaagattt
     1381 ccttggtaca tcacagcaat aaattcgcca ctttgccctt ccaccgcagc gggagcccac
     1441 acgggtgttt gaggttccag cccattctta tccgcaggcg gaaagagtgc ccgactgttt
     1501 gaaccatccc ggtccatcac aaccaaacga tagcggcttg agtcgctctg gtcgatgaag
     1561 atggactgta gataggcaat ctgaaatgcc gcttctctac ttccatattg gatcggagaa
     1621 atggcaggat atgagaacat gcctgctgaa ttgaccaaag acactgccgc ctcattctta
     1681 aacgagatgg ctgagaggtc aaaataaggc gactcctcgc cggaaatggg tgccggagcc
     1741 ggagcatggc tgacgaagta taaaccgttt ccgtctgcgc cccaaacgag aggcggaatc
     1801 cacgcccagt cactatgcgt gttcagcgga gtaatatcta gtaatggttt taagcgaccg
     1861 ctatcttgat ccactaagcc aattccatca ggacgagaat aagcgagcct accatctggg
     1921 gcgaaggcaa aattcatgcc ccaccaacca tacacaccac cactgcttgg atataagatg
     1981 cgtttgggtg aaccactgtt tgtaatgctg acgcggtaca ggtcattgtt tgcttgccaa
     2041 cctggtgccg cactgcgcgg ctcaacggtg gaatacgcga tcgaattcgt gccaggaatc
     2101 catgctgcaa agtggactac attttttgct tgcaatgaga tcaatttagg gtcaaggttg
     2161 cgggtgcgaa ctgcccaaag ggtattgatc tcttcgtctg ctggcttgct ggatttgcgc
     2221 gtaaaaacca aatattcacc gttaggcgag agagtgaaga tgcgcccatc taaatctccg
     2281 gtgctgacga taacacggcg attggcggtg gagccttcca tgatccaagc attgccgcca
     2341 gctagatagg ctaacgtgcc gggaatgttt agcggcacaa aagaagtttg tgcgccgacc
     2401 atgacaatct caggtaaagc ttctttgatc accgtagttt ttacaatgga ctgactggtt
     2461 tctactttat cttctaatat cttgcgatat gtgatttctt caagtccgtt cacgccagcc
     2521 tgaacgagac gggtttcgcc ttcgggcaag gtttcgttgc ggacgacttg tcgttcaaac
     2581 gggacgacga tctcttcggt ttcgaaaact tcttccaccc gaattaaggt gacgaggtcg
     2641 ccattgccaa tgacggtata gagcggcggc tctgtcttat cgaggctgtt tacggtgatc
     2701 cctgcacttt ccaacgcctg tgagacggtt tgcccttgcg gaacagtgat gtctctagta
     2761 gtgccatctg cagtgactgt gattgtaatg tcactattga gctgcggcga cttacaagaa
     2821 gccaacagtg taaccaaaag caagatggtg gcgagcaagc tcgggcggag ggtgaggcgg
     2881 ggcatgtcgg gtataatcca gccgctattt gatccgcccg gtgaggggca tgatgccgtc
     2941 tgcgatggtg atggtttgta atcgaaagcc aacagctacg ggaccgaatg aaccggtgaa
     3001 ggcttcttca ataatggcgc taatggcgtt gttgacacct tctggcgcag ggatgggacc
     3061 aaagtctgca gatgtgatct gtattttggg ttgacctgtg gtctcatcga tcgttacgtt
     3121 catggtaatg agcatattgg ctgtaaacca accctgtgta accttgccat acatttgcat
     3181 ttgcccgttg cgaagcaaga tttgtggatc agtaaacggt gggtttgttt gttgagaaag
     3241 tttttctgcc atatagccgg ttagctgact ttcggtgacg gaaagggtga tcacgccggt
     3301 ttctgcacca gtgaccatgg cttgttggat catatcgcgt agtgcttggg cgtcggttgg
     3361 tgaggctgaa atgggctgag ccggatattc tggtccgccg acattgatag agcatgctag
     3421 tgatgcactg acaagcgaaa caataaaaag gtaaatatag tttttagttt tcatgatttt
     3481 gtgaacgttt gaaatatgga ctgcggagca attatagcat gagcgaatct gaacccatta
     3541 caaatgaaga attgagcctg gcggcaaaaa tcgaggcgat gttattcgtt tcagcagacc
     3601 cggtaccagt gcaacaactg tcgcaggcgt tggatgtgac tgcgtctgtt gtggaacgtg
     3661 ggctgaaaga attagacgaa gcattaaaat ctcgtggctt gcggcttcag cgcaatgcag
     3721 ggcgtgtgca gttgaccacc gcccctgaga ttgctggatt ggtggaaacc ttccttggat
     3781 tggaggctat tacgcacctc agccgagcgg cgttagaaac attggcgatc attgcttacc
     3841 aacagccggt gacgcgtcca cagatcgatt cgattcgtgg tgtgaatagc gacggtatga
     3901 tgaaaagctt gttgagcaaa ggtttgattc aggaatctgg acgcacagaa ggtccgggtc
     3961 gtcctattct ttattcaacc acgccggaat ttttacagca ctttggtttg agctccatta
     4021 tggaattgcc gccattggca actccaattg aaaccgaacc tgaaccgcaa gaattgttga
     4081 aaggttaatt atgcaggaac gtttgcaaaa gattctcgca caggctgggt atggttcccg
     4141 ccgtgcttgc gaggagttta ttattgaggg gcgagtttcg gtcaatgggc agattgcaat
     4201 cttggggcag aaggcagatc tggcagtgga taccattaag gtggacggta agtcactttc
     4261 aaagcctgaa acgatgacgt atatcgcctt atacaagcct cgtaatgtcc tctctgctgc
     4321 agaggggcaa gacgatcgta aaacggttcg agatttgatt cccgctcctg gacatttata
     4381 tccagtagga cgcctcgatt ttgactccga aggcttgatt ctcatgacca atgatgggga
     4441 tctcacgaac aagttgacac atccgcgata cgcacatgaa aaagaatatc gcgtgctgct
     4501 cgcttgtaaa ccggatactg aacagcttga tgcttggcgc cgaggcgttg ttcttccaga
     4561 aggtgaccgt acccagcctg cgaatgttca ctttatttcc acatctggca aaggtgcatg
     4621 gattcgtgtg gtgatggggg aagggaagaa acgtcagatt cgggaagtgg gtaaattgct
     4681 tggtttgcct gtggtacgca ttattcgtgt gcgtatcggc acattgaaat taggtgattt
     4741 gaaaccccga caatggcgat atttaactga ggatgagatt cgggaattaa aaggggataa
     4801 ggttccgaag atggagca
//
LOCUS       DAOREF010000099         4805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10074_length_4805_cov_4.154105, whole genome shotgun sequence.
ACCESSION   DAOREF010000099 DAOREF010000000
VERSION     DAOREF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10074_length_4805_cov_4.154105"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKI33_03060"
     CDS             complement(<1..336)
                     /locus_tag="PKI33_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNM36010.1"
                     /translation="MKLVTVSQMRAIEKEADAKGISYAQMMQNAGCGLAEIVHVIGQE
                     HGWTNVVGIVGSGNNGGDALVALTWLAKAGWKTQAYVVKRQDAGEELVEKYLDAGGQI
                     LQYADDVSLN"
     gene            404..1492
                     /locus_tag="PKI33_03065"
     CDS             404..1492
                     /locus_tag="PKI33_03065"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNM36011.1"
                     /translation="MNPKQETLLKRATLLNENPRLKKNNFDLLRLALAGIVCFVHAYQ
                     LSGYESLSFIPKILSSEAAVKGFFVASGFLIFMSYERSSSLLSYFSRRIRRIYPAYLT
                     IIIIMAFGFVLISTKNAAEYFFSSEWIKYLAANLVFLNFLQPSLPGVFESNIFTAANG
                     ALWTLKIEVMFYFSVPVIVYLFKYIPKNWVLFGGYIFSIIYSTAFNIIASQTGKELFQ
                     EIGRQLPGQLCYFLAGAFFYYNLSIFEKYITYFIVSSSIALIINQFYTIHWLEPFALA
                     TLIMFFSLFLYIGEIGKYGDFSYGIYILHSPILQIFIQYRWFSENPWLYLLIGLLTTL
                     ILAILLWHGVEKRFLLRNSHYLSNPTRS"
     gene            1508..2548
                     /locus_tag="PKI33_03070"
     CDS             1508..2548
                     /locus_tag="PKI33_03070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNM36012.1"
                     /translation="MQKLKSIQALRGIAVITVVLLHLKLVERKYSPTNFLLPDWLDFG
                     IFGVDLFFVISGFVMVTVTQEKFQSSKSSLLFLYHRVSRIYPLYWVYTTIALIVFKIQ
                     PSWVNSSQGNQANILSSYLLLPDNLLPLVQVGWTLIHEMYFYVVFFAIMLFLPGKYLP
                     IALLLWGGTIAMFGPSTINPFYNLILHPLTIEFIAGCFIAMYFNYVQPFRLNGLLLIF
                     FSFISILVAILSFVYFRSQTGYAPDNWDRVLYYGIPAALIVFFIVNAEKNSLSLPQFL
                     IQVGNASYSIYLSHLFTINAIGKIWSIFNSSSIIDNFFIVPATIGMVLFIGFASYWYV
                     EIPLLNLSRKLA"
     gene            complement(2550..3338)
                     /locus_tag="PKI33_03075"
     CDS             complement(2550..3338)
                     /locus_tag="PKI33_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNM36013.1"
                     /translation="MEILTSTSNPFIKQIRALRQKKTRVETGTFLVEGITHVGEVIEA
                     GWDVDAIIYAPDLLTSRFAKDLLARAARLQLRLQPVSVSVIESLADKENPQGILAVVK
                     QKPTKFSNLKSVNRAVALVSPQDPGNLGTILRTMDAVGADTLFILDGGVELFHPTVVR
                     ASMGTLFWKPVVQASFSEFLEWARTNHCQLIGTSAKADVDYHTLVPQVPWVLVLGTEQ
                     KGLTPEQTTACDVTISLPMRGRVSSLNLSVAAGVLLYQFMGDRD"
     gene            3531..4124
                     /locus_tag="PKI33_03080"
     CDS             3531..4124
                     /locus_tag="PKI33_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNM36014.1"
                     /translation="MEKFLKSMNLSYEDWRNGQSYDLNVLKNLSPAETQEAERILISR
                     KDEDFRDVEALAALGTPAAIQALRDCLQSRNLSNRFHAAKYLKETGEDHVEEVIFSTL
                     HITQIGNGMVEGLRLAKENPSDKIKHRVVWCTFHGNDGIRTHCAAMALYLYGKADSDF
                     DNKQQIIFRVGAKNKEMGNKVFPEFCELLGVNPQDFA"
     gene            4194..>4805
                     /locus_tag="PKI33_03085"
     CDS             4194..>4805
                     /locus_tag="PKI33_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HNM36015.1"
                     /translation="MKLAPWLVLLLAFSLVSCQSLSPRSVTILDGDTVLPVTTSETVP
                     LLILADASITPQPTDRVLVNGIPYKLDQDISLDGDWHLQLIRSISVKLITPQGEQNLQ
                     TSALTVGDFLKENGFATSLNDKVTPPLTTPITDSLTITFSPAQTMSISAGETSLTINS
                     SAQTVGEALAQAGIPLMGMDTSVPAENEPLPIDGQITVTRVTES"
BASE COUNT         1387 a         1015 c          901 g         1502 t
ORIGIN      
        1 attcaagcta acgtcatcgg catattgtaa aatttgaccg ccagcatcaa ggtatttttc
       61 aactaattct tcacctgcgt cttgacgttt caccacgtag gcttgagttt tccatcctgc
      121 tttggcaagc caagttaaag caacgagtgc atcgccccca ttattccctg aaccaacaat
      181 ccctacaacg ttagtccaac catgctcttg tccaatcaca tgaacaatct cagccaaacc
      241 acacccagca ttttgcatca tctgagcata agagatgccc tttgcatcag cttctttttc
      301 aattgctcgc atttgagaaa cagtgacaag tttcatatca attcatagtt attacaggtt
      361 tataattgtt ttctgctact aaattgtaca ttgctataca aacatgaacc caaaacaaga
      421 aacactattg aaaagggcta cattgctaaa cgaaaaccct cgccttaaaa aaaacaactt
      481 cgatttactt cgcctagctc ttgctggcat cgtatgcttc gttcatgcgt atcaattatc
      541 tggctatgaa tcgctaagtt ttattcctaa aatattatcc tctgaagcag cagttaaagg
      601 tttttttgtt gcgagcggct ttctaatctt tatgagctat gagcgatctt cttctttact
      661 ttcatatttt tcaagaagaa ttaggcgcat atatcctgca tacctaacca ttatcatcat
      721 aatggcattc ggtttcgtgt taataagcac caaaaatgcg gcggaatatt tcttttcatc
      781 tgaatggata aaatatctag cagctaatct tgtctttcta aattttcttc agccatcact
      841 tcctggtgtt tttgagtcta atatatttac agccgccaat ggtgcactat ggacgctaaa
      901 aattgaggtt atgttctatt tttcagttcc agtcattgtt tatcttttca aatacattcc
      961 taaaaactgg gtattatttg gtggttacat attttctatt atttattcaa cagccttcaa
     1021 tataattgca tctcaaactg ggaaagaact atttcaagaa ataggccgtc agttaccagg
     1081 gcagctatgt tattttctag caggagcttt cttttattat aatctttcta tttttgaaaa
     1141 atacatcact tatttcatag tctcatcctc aatagcgcta atcataaatc agttttacac
     1201 aattcattgg ctagaaccat ttgctttagc tactctcatt atgttcttta gtttattttt
     1261 gtatataggc gagattggaa aatacgggga tttctcatat ggcatttata tattgcactc
     1321 tccaatactt caaatattta ttcagtacag atggttcagt gaaaatcctt ggctttatct
     1381 cttaataggt ttattgacaa ctttaatatt agccattttg ttatggcacg gggtagaaaa
     1441 acggtttctt ctacggaata gtcattatct ttcaaatcca acaagaagtt aggaatttac
     1501 ccataaaatg caaaagctaa aaagtattca agcactgcgg ggtatagctg ttattacggt
     1561 tgtattactt cacttaaaac tggttgagag aaaatatagc cccacaaatt ttttattacc
     1621 tgactggcta gattttggaa tctttggtgt cgaccttttc tttgttatca gcggatttgt
     1681 catggtcact gttacccaag aaaaattcca gtcctcaaaa agctctttac tattcttata
     1741 tcatcgtgta tcaagaattt atcctcttta ttgggtatac accacaatcg cattaattgt
     1801 ttttaaaatt cagccatcat gggtaaacag ttcacaagga aatcaggcaa acatcttatc
     1861 gtcctatctt cttttaccag ataatcttct tccattagtt caagtcgggt ggactttaat
     1921 tcatgaaatg tatttctatg tagtattttt tgcgatcatg ctttttttac cgggaaagta
     1981 tctgccaatc gccttattac tatggggagg gacaattgcg atgtttggtc cttcaacaat
     2041 caatcccttc tacaacctaa tactccatcc acttactata gaatttattg cagggtgttt
     2101 cattgctatg tactttaatt acgttcagcc attcagattg aatggcttgc ttcttatttt
     2161 cttttctttt ataagcatat tggtcgcaat acttagtttt gtttattttc gaagccaaac
     2221 tgggtacgct ccagataatt gggaccgggt tctttattat ggaataccag ccgctttaat
     2281 tgtttttttt atagtcaatg ctgaaaaaaa cagcctctca cttcctcaat tcttaattca
     2341 agttggaaat gcttcctact caatatatct ttctcattta tttactatca atgccatagg
     2401 gaaaatatgg agtatattta attcatcatc aattattgat aattttttta tagtaccagc
     2461 cacaattgga atggttctgt ttatcggttt cgccagctat tggtatgttg agatacctct
     2521 tcttaactta agccgtaagc ttgcatagac taatcacgat ctcccataaa ctgatacaac
     2581 aagacacctg ctgccacaga caagttcagc gagctgactc gtccgcgcat ggggagagaa
     2641 atagtcacat cacaggctgt cgtttgttct ggggtaagcc ccttctgctc agtacctaac
     2701 accaacaccc aaggtacctg tgggacgaga gtgtgataat ccacatccgc tttggctgag
     2761 gtcccaataa gttgacagtg attcgtacgt gcccattcaa gaaattcgct aaaagaagcc
     2821 tgtacaaccg gcttccaaaa caacgttccc atactagcac ggaccacggt gggatgaaac
     2881 aattccacgc cgccatctag aataaagagt gtatctgcgc caaccgcatc catagtgcgg
     2941 aggatggtgc caagattgcc agggtcttgc ggcgaaacca aagctactgc gcggtttaca
     3001 gactttaggt ttgaaaactt cgttggcttt tgcttaacta cagcaaggat tccttgtggg
     3061 ttttctttat cagcgagaga ttcgatgaca gaaacagaga caggctgaag tcgaagttgg
     3121 aggcgggcag cgcgggcgag caagtctttt gcaaagcggc tggttaacag atcaggcgca
     3181 taaatgatgg cgtccacgtc ccagcctgct tcgatcactt ctccaacatg tgtgatgcct
     3241 tctacaagaa aggttccggt ttctacacgt gtttttttct gtcgcaaagc gcgaatctgt
     3301 tttatgaaag ggtttgaggt gctggtaaga atttccatat ctgaatttta tccgatatga
     3361 agtgatttgg tgggaggcag acttttgggg gatgaaattt aaaataaaga tacatataat
     3421 ttcagcatat tcaattcaaa cgaggaaata attttatgtt caaaagttta tttggaaaga
     3481 aatcgtcaga aacaagcaaa acgccatcta caggctcgca aagtgaaggt atggaaaaat
     3541 ttctcaagag catgaatctt agttatgaag attggcggaa tgggcaaagt tatgatttaa
     3601 atgtattgaa aaacttaagc ccagcagaga cacaagaagc agaacgaatt ctaatttctc
     3661 gaaaagatga agattttcga gatgtagaag cattagctgc acttggcacg ccagccgcca
     3721 ttcaagcgct acgagattgc ctgcaaagcc gaaatctttc aaatcgtttt catgcggcaa
     3781 aatacctgaa agaaactggc gaagaccatg ttgaagaagt cattttcagc acgttgcata
     3841 ttactcaaat cggtaatggc atggtggaag gattaagatt agcaaaagaa aaccccagtg
     3901 acaaaatcaa acaccgcgtg gtctggtgca catttcatgg caacgacggt attcgcactc
     3961 actgtgcagc aatggctctt tacttatacg gcaaagccga ctctgatttt gacaacaaac
     4021 aacaaatcat ctttagagtt ggagccaaga acaaagagat gggcaataag gttttcccag
     4081 aattttgtga gcttctcggt gtaaacccac aggattttgc ataaatctcg aaaacgaccc
     4141 gatcaaattt tcgggtcgtt tttttatgcc tcaatccatt tataattggc agtatgaaac
     4201 ttgctccatg gctggtcttg ctactggctt tttcgcttgt cagttgccag tcgctctccc
     4261 cacgttcagt cacaatttta gatggcgata ctgtgctacc cgttacgacg tcagaaacgg
     4321 ttccactttt gattcttgca gacgccagca ttactcctca gccaacagat cgagtccttg
     4381 tgaatggcat tccatataag ctcgatcaag atatttctct tgacggtgat tggcacttac
     4441 aacttatccg gtctatttcg gtaaaactga ttacgcctca aggggaacag aatcttcaga
     4501 cctccgcatt gactgtaggt gactttttga aagaaaatgg attcgccacc agcctcaatg
     4561 acaaagtcac cccgcctctt accacgccga taactgattc attaaccatc actttttctc
     4621 cagctcaaac aatgagtatt tcagcaggag aaacatctct cactataaat tcttctgctc
     4681 aaactgttgg tgaagcactg gcacaggcgg gaattccgct catgggcatg gataccagcg
     4741 tcccagcgga gaatgagccg ctcccaatag atgggcaaat taccgttacc cgggtgacag
     4801 agtca
//
LOCUS       DAOREF010000100         4796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10111_length_4796_cov_5.245729, whole genome shotgun sequence.
ACCESSION   DAOREF010000100 DAOREF010000000
VERSION     DAOREF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10111_length_4796_cov_5.245729"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKI33_03090"
     CDS             <1..259
                     /locus_tag="PKI33_03090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNM36016.1"
                     /translation="NGVVVFAGPWTVHGNATVIDHGWGIYSGFWHQSAIQVQVGQTVN
                     QGDVIGLVGGTGRVTGAHLHWELWVNGVQVDPLDWLNQEYP"
     gene            306..800
                     /locus_tag="PKI33_03095"
     CDS             306..800
                     /locus_tag="PKI33_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNM36017.1"
                     /translation="MNNLVNFLKQSDIFYQFTPTQLELVANLCQEAEFEANDTIFQEN
                     SSSKELYVIVQGEVDILINRGTTGSLGKSETVIARLRRGQSFGEVALVDEGLRSASAR
                     AAVKDTRLLVISRDKIVMLCETYPQLGYRLMYNLAADLAMKIRNTDLRIREQLLYKPA
                     ENKN"
     gene            1100..2368
                     /locus_tag="PKI33_03100"
     CDS             1100..2368
                     /locus_tag="PKI33_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNM36018.1"
                     /translation="MNKRLFALLSLLVIASMALAACGTPAATEAAATEAAATEAAATE
                     AAPAESMGTIKIATQSPLSGGQSLLGVAIKQGAELALEQLAGDLTAMGYDVQLAPYDD
                     QATPDVGVANAKNIVADADILCGVGHLNSGVMIPSSEEYHTAGLAFISPANTNPVVTT
                     RGYLEVNRVVGRDDVQAPAAEEYAFKTLGAKSVYIIHDKTAYGQGVAEFFKAAAEEDG
                     IEVMGFEGTEETANFDAIVTPLIALNPDVVFFAGIYNQAGVFFKQARDAGYEGTFLGT
                     DGMDSSDLADIAGDALTTGGGMAYTTVAGPASAYPAAAQFVADYTAKFGAAPEAYAAQ
                     AYDSAGICLTAIKAAAEAAGGKPTRAQVAEAIRAVNYEGLTSTITFDEIGDLPAAKYF
                     IIKVNASSKDAWGTNEIIDSFDLPSPGKGE"
     gene            2443..3576
                     /locus_tag="PKI33_03105"
     CDS             2443..3576
                     /locus_tag="PKI33_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNM36019.1"
                     /translation="MKQFTANPVYKFFIFPIGQIIVFALGASLVLSLLTVLLANVFKF
                     NVTDFDLVERTLLILPQVVINGLTIGFVYSTIALGYTMVYGVLEFINFSHSEIFMVGG
                     IVGIQLMAYFEHIGVLATMNQYLVIALAIILGMVISGSLAVTVERVAYRPLRNAPKLV
                     PLISAIGMSFFLQDLVRLILSLTTGEFNVILPKFGTFDDFWKLAIPGMEDPLRIQIKS
                     VIMVGIAILMLVGLNFLVNGTKVGKAIRAVAQDRPSASLMGINVNQIISITFLIGGAL
                     GGAAGVLFALKATKLDPFSGFFPGLKAFTAAVLGGIGNLTGALLGGLVLGLLESFAAS
                     YLSIFTNGAFGAEYKDIFAFAILIVVLVFRPSGILGQTVGQKA"
     gene            3603..>4796
                     /locus_tag="PKI33_03110"
     CDS             3603..>4796
                     /locus_tag="PKI33_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36020.1"
                     /translation="MKTFFSDLYKKLNTGSWPIVVALAFLIVGTIAVYLSPRSLTAFL
                     ILMATPVILYFMKMPVKVKIFIGLLLIIVVFPVLGIRNIFYLEVLFQIAVAAALALGL
                     NIVIGMAGLLDLGYVAFYAVGAYAWAIFGSKQMSLLHSIPGAAPANAPFFLDAKWFWL
                     FIFLGVGLAALTGILLGLPVLRVKGDYLAIVTLGFGEVIQVLALNLDKPINLTNGPQG
                     ITPIQRPPLPPDGIMQFFRTILTPLVGHSPTDAEFYNVMFYFLALVVIGIAIVVATRL
                     EDSRIGRAWTAIREDETAAIAMGIPLVKMKLAAFAAGASFAGAMGALYAANRTFVSPE
                     TFDFQVSIGVLTYVILGGLGSIPGVIFGAATVVLLNIQVLQNFSLYLSELRQSDAVIP
                     IINFAW"
BASE COUNT          999 a         1298 c         1175 g         1324 t
ORIGIN      
        1 aaatggggtc gtcgttttcg ccggtccttg gacggtgcat ggaaatgcaa ccgtcattga
       61 ccatggctgg ggcatttaca gtgggttttg gcatcagtct gcgatacagg tgcaggtggg
      121 gcaaacggtc aaccagggcg atgtgattgg tctggttggc ggaacagggc gtgtgaccgg
      181 ggctcacttg cattgggagt tgtgggtaaa cggcgtccaa gtggacccgc ttgactggtt
      241 gaatcaggaa tacccgtaga ttcgaggcgt tgcacttcat cctctaatat gctaaacttt
      301 ccgacatgaa taaccttgtc aacttcctca agcagagcga tatcttttat caattcacgc
      361 caacccagtt ggagttggtt gctaatctgt gtcaggaagc tgagtttgaa gcaaacgata
      421 ccatttttca agaaaatagc agcagcaaag agctgtatgt gatcgtccaa ggtgaagtgg
      481 atattcttat taatcgcggt acgactggtt cgttgggcaa aagtgaaaca gtgatcgcac
      541 gccttcgcag aggtcaatcg tttggtgagg tggctttggt agatgaaggg ttgcgttctg
      601 cctctgcccg agcagccgtc aaggatactc gcctgctggt gatctcacgc gataagatcg
      661 tgatgctgtg tgaaacgtac ccgcaacttg gttatcgttt aatgtataac cttgcggcag
      721 atctcgccat gaaaattcgc aataccgatc tacgtatccg cgaacagttg ctttacaagc
      781 ctgctgaaaa taagaattaa tactgcgtga tgtagataaa tcccctctaa ttttactgaa
      841 aagtatagac acaaacgctt gaccgatgtt aaaaatcgac gtattattag ccattcgtac
      901 atttggttaa tggtattcag gtaacgtcaa acaagccata aaaatccatt gagttagggt
      961 tttttggctt tctttgtata tcaaccaatt tataaaagga ggtgatgccg actcacccgc
     1021 gcttggtctg ctagtttgtc gtacccgcgt attgcgggaa tatacatcaa cctattgtaa
     1081 attttggagg agtaagataa tgaacaagcg tttattcgct ttgctgtcac ttttggtgat
     1141 tgctagcatg gcactcgccg cctgtggcac ccccgccgct accgaagctg ctgctactga
     1201 agccgccgct accgaagccg ctgctaccga agccgcccct gctgaatcca tgggcaccat
     1261 caagatcgct acccagagcc ctctctccgg tggtcagtcc ttgctcggcg ttgccatcaa
     1321 gcagggtgct gaactcgccc tcgaacagct cgctggtgat ctcaccgcta tgggctatga
     1381 tgtccagctc gccccgtacg atgatcaggc gacccccgat gtgggcgttg ctaacgccaa
     1441 gaacatcgtt gctgatgctg acatcctctg cggtgtcggt cacctcaact ccggcgttat
     1501 gatcccttcc tctgaggaat atcacaccgc tggtctcgcc ttcatctccc ccgccaacac
     1561 caatcctgtt gtgaccaccc gtggttacct cgaagtgaac cgtgttgtag gtcgcgatga
     1621 tgtccaggct cctgccgctg aagaatatgc tttcaagacc ctcggcgcca agtccgtcta
     1681 catcatccac gacaagaccg cttatggtca gggcgttgct gaattcttca aggctgctgc
     1741 cgaagaagat ggtattgaag tgatgggctt cgaaggtacc gaagaaaccg ccaacttcga
     1801 tgcgatcgtt acccccttga tcgccctcaa ccctgatgtt gtgttcttcg ctggtatcta
     1861 caaccaggct ggtgtgttct tcaagcaggc tcgcgatgcc ggctacgaag gcaccttcct
     1921 cggcaccgat ggtatggatt cctccgacct cgctgacatc gctggtgatg ctctcaccac
     1981 tggtggcggt atggcttaca ccactgttgc tggtcctgct agcgcttacc ccgctgctgc
     2041 ccagttcgtt gctgactaca ccgccaagtt cggtgctgct cctgaagcct acgccgctca
     2101 ggcttatgac tccgctggca tctgcttgac cgcgatcaag gctgctgctg aagctgctgg
     2161 tggcaaaccc acccgcgctc aggttgctga agccatccgc gctgtcaact acgaaggtct
     2221 caccagcacc atcaccttcg atgaaatcgg cgaccttcct gccgccaagt acttcatcat
     2281 caaggtgaac gcttcttcca aggatgcttg gggcaccaac gaaatcatcg actccttcga
     2341 ccttccctcc cccggtaagg gcgaatagtt cgaaccctgt aataaaagcc tctctcccgt
     2401 atgggagaga ggctactttt caaaaagaca ggattgaatc acatgaagca attcactgca
     2461 aatcccgttt ataagttttt tattttcccg atcgggcaga tcattgtatt tgcactggga
     2521 gccagcttgg tcctgtcgct gcttaccgtg ctgctcgcga acgtcttcaa attcaatgtc
     2581 actgactttg accttgtaga gcgtacttta ttgatcttac cgcaagtggt aatcaacggg
     2641 ctaaccattg gctttgtcta ttccaccatc gccttagggt acaccatggt gtatggtgtt
     2701 ttggaattca tcaatttttc ccatagtgaa attttcatgg tgggcggtat tgtgggcatc
     2761 cagttgatgg catactttga acatattgga gttttggcta cgatgaacca ataccttgtg
     2821 atcgcacttg cgatcatcct cggtatggtc atctccggct cactggctgt gaccgtggaa
     2881 cgggtcgcct accgtccact tcgcaacgct ccaaagttgg ttccgcttat ctctgccatc
     2941 ggtatgtcct tcttcctgca ggaccttgta cgtttgattc tcagcctcac cacgggcgag
     3001 ttcaacgtca tcctccccaa attcggtacc ttcgacgact tctggaagct ggcaattccg
     3061 ggcatggaag atcccctacg cattcaaatc aagtctgtca tcatggtagg catcgccatc
     3121 ctcatgttgg ttggcttgaa cttcctcgtc aacggcacta aggttggtaa agccatccgc
     3181 gccgttgcgc aggatcgtcc ttccgctagc ttgatgggca tcaacgtcaa ccagatcatt
     3241 tcgatcacct tcctcatcgg tggtgctctg ggtggtgcgg ctggtgtatt gttcgccctt
     3301 aaggctacga aacttgatcc cttctctggc ttcttccccg gtttgaaagc atttacggca
     3361 gccgttcttg gtggtatcgg caacctcact ggcgccctct tgggcggatt ggtgcttggc
     3421 ttgctcgagt cttttgcggc ttcctacctt tcgatcttta cgaatggtgc tttcggcgct
     3481 gagtacaagg atattttcgc gtttgccatc ctgatcgttg tgttggtctt ccgcccctcc
     3541 ggtattctcg gtcagaccgt tgggcagaag gcttagtccg tattcaggaa aggtacacaa
     3601 ccatgaaaac atttttcagc gacttgtaca agaaactcaa tacgggctcg tggccaatcg
     3661 tggtcgcact tgcctttctc attgtaggaa cgatcgctgt ttatctcagc ccacgttccc
     3721 tcacagcctt cttaatcttg atggcaaccc cggtcattct ttactttatg aaaatgccag
     3781 tgaaagtaaa gatcttcatt gggttgcttt tgattattgt ggtcttcccg gtattaggca
     3841 ttcgcaacat cttctatttg gaagttctct ttcaaatcgc tgttgcagcc gcacttgctc
     3901 ttgggttgaa catcgtgatc ggtatggcag gcttgttaga ccttgggtat gtggcgttct
     3961 acgcagtggg cgcttacgct tgggcgatct ttggttcaaa gcaaatgtct ttgttgcact
     4021 cgatccccgg tgcggctcct gccaatgctc ccttcttcct cgacgcaaaa tggttttggc
     4081 tattcatttt cttgggggta ggtttagctg ctctaaccgg catcttgttg ggtctcccag
     4141 ttctgcgcgt aaaaggtgat taccttgcca tcgttacact tggcttcggt gaagtgatcc
     4201 aagtattggc gctcaacctc gataagccca tcaacttgac caatggtccg caaggcatta
     4261 cccccatcca acgcccgccc ctgccaccag atggaattat gcagttcttc cgcacgattc
     4321 tcaccccact ggtagggcac tcccctacag atgctgagtt ctacaatgtg atgttttact
     4381 tcctggcgct cgtcgtcatt gggattgcaa tcgttgtagc aacccgcctc gaagactctc
     4441 gcattggacg cgcttggaca gccattcgcg aagatgagac cgcagccatc gctatgggca
     4501 ttccgctcgt gaagatgaag ctcgccgctt tcgccgccgg tgcctctttt gctggtgcca
     4561 tgggcgctct ctacgctgcc aaccgcacct tcgtcagccc tgaaaccttc gacttccaag
     4621 tttccatcgg tgtattgacc tacgtcatcc ttggtggttt gggcagcatc cccggcgtga
     4681 tcttcggtgc cgcaaccgtg gtgctcctca acattcaggt gttgcagaac ttctctctct
     4741 acttgagcga acttcgccaa agcgacgcag tgatcccgat catcaacttc gcttgg
//
LOCUS       DAOREF010000101         4778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10178_length_4778_cov_3.111582, whole genome shotgun sequence.
ACCESSION   DAOREF010000101 DAOREF010000000
VERSION     DAOREF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10178_length_4778_cov_3.111582"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKI33_03115"
     CDS             <1..569
                     /locus_tag="PKI33_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNM36021.1"
                     /translation="SALPNCTILQPCSAEETRMAMDYAVNKTDDVVALRLIIGPSPRR
                     ISLPAGYEFKTGQGCQLTTGKDAVMFAYGPVMLHEALLASELLAERGFGLSIINMPWL
                     NRVDVKWLTEVLKPYSNIFMLEDHHPAGGLMDNILTACSENGLLNGKQMIKFAVEGYP
                     ACGTPQQALAFHELDGLSLSKRVLKYCG"
     gene            575..1993
                     /locus_tag="PKI33_03120"
     CDS             575..1993
                     /locus_tag="PKI33_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNM36022.1"
                     /translation="MENIKIGIPLHGKMSSRTFHSALFRERLGEKGFEPIYFLTSDYL
                     EASPYDPKRYFELKVSAYEAYYEKHALLRNMKSLRRFVIVTETTDLRLREEIESIIFQ
                     KDLWKIGGYILYTSILRWVPGMGDFLLGLENLLYKTDAHTEVIRNIPLNCVLTPGMGN
                     YRFEYSGQFSLEAQRLGIPVFSAVTNYDNIVNMGFRGFTPKCIAVWSQQMADELMRLH
                     KMSGKRIEVTGPIQYDRFKQPLPRSREEFLESIGLDPKKKTIFIAGGVNITRYFELFN
                     LFFEQQTNVFSEPCNIILRPYPHVKLLSSPGWKILRKLFLDRGVYISIPDAIDSHEHR
                     SQELKSDLYSEEEHDELNYLLRYSDVMVNYFSTISLEAAICDLPVIHVGYDIYTYGHR
                     FHVTSEFLQRQTHNRRKFRLAASRVAKTEKDLVKYINMYLSDKSLDRDARYEYAISEC
                     GELDGHAGRRIVQMIKSRLQRQ"
     gene            2003..2662
                     /locus_tag="PKI33_03125"
     CDS             2003..2662
                     /locus_tag="PKI33_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36023.1"
                     /translation="MNVMQKLVQEIKKSTHLLPPLQRWIDHQEQAADLAYWQMKPRTL
                     PLPHLLKQNILKGYAIEYKLPVLIETGTYMGEMVEALRNVFKTIYSIELSDKFAQRAK
                     KYFRYSSNVTILQGDSGEVLRELIPKIDQPALFWLDGHWSADQTARGKKSTPIMEELD
                     HILHAKDLGHVILIDDARAFGKSEDYPTLDTLSDFVLSLRKNVKIISEDDIIRILPQY
                     E"
     gene            2655..3638
                     /locus_tag="PKI33_03130"
     CDS             2655..3638
                     /locus_tag="PKI33_03130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNM36024.1"
                     /translation="MNKPSLLTIGILSWNRLNYLRATLESAKRCIQYPNIQWIVLDNY
                     STEPGLAEYLKSLAWIDELIFMQSSHVAAMNEIVARAKGDVLLLWPEDVQFVVEGDWM
                     RDCIEILLKDYSIGALSLNFHRRQTIEYIWGRQRFNKNGILRMWKDLKRYGANFRLQK
                     KNISSNGYPVRTFGWQEDGIIGAGITSLARTEVWKKLGPWKAKPADDNIIDSSGGGET
                     EMLERWAKTKTPLQRALPILPVSADIINDNIGSKAKVRGNKRYGVYTPPLQGDFYYKI
                     YEQKDVANLQKRNLPVSFEEFVVPIGFGLPLDSEGNLLKSSFNDTIVADVN"
     gene            3756..>4778
                     /locus_tag="PKI33_03135"
     CDS             3756..>4778
                     /locus_tag="PKI33_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNM36025.1"
                     /translation="MQNIKSVLGVTLGHDTSFAHVVNGKIVAIMEAERYFRQKRYKLH
                     SQTMEPGKHISGYQYVSLEDMELFLGLVAKEWGNTFDALAVQNQGRIEEFKNFQQILS
                     RLGFKFSSVHHVDHHLSHAALSFFSSPFKEALVLSYDGEGNDGQTIIFGAKGNKLEYL
                     HNDPIRFGQSYNNMGYIVGIKPEVEGTTSGKVMGLTAYGEPVEDWMPYAREYIKKYIK
                     ATPRQIEGLNKYGKAHRINDIAVRDIPDLKQYLVPEEDSDGGLKSAVKKMIGRTNTIL
                     RLNGPEDKTAQNFAATVQVAWTKAVIDLLKPYFGKFENLCVVGGCALNGVTNYEIQQL
                     GVFKATH"
BASE COUNT         1421 a          870 c         1083 g         1404 t
ORIGIN      
        1 tctccgcgtt gccgaattgc accatcttgc aaccttgcag tgccgaagag actcgtatgg
       61 cgatggatta cgccgtgaac aaaaccgatg acgtggttgc cctgcgcctc atcatcggac
      121 ccagtccgcg ccggatttcg ctcccggctg ggtatgagtt caagaccggg cagggctgtc
      181 aactgacgac ggggaaagat gcggtcatgt ttgcatatgg tcccgtcatg ttgcacgaag
      241 cactcctcgc cagcgaactc ctcgccgagc gcggattcgg cttgagcatc atcaatatgc
      301 cctggctcaa tcgcgtggat gtgaagtggc tcactgaagt cctcaagcct tactcaaata
      361 tcttcatgct cgaagatcat caccccgctg gtggtttgat ggataatatt ctcacggctt
      421 gcagtgagaa tggtttgctc aatggcaagc agatgatcaa gtttgcggtg gaaggttatc
      481 ctgcctgcgg tacgccgcaa caggcgttgg cgttccatga attggatgga ttatccttgt
      541 ctaagcgtgt gctaaaatac tgcggataaa gtaaatggaa aatataaaaa ttggaatacc
      601 tctgcacgga aagatgtctt ctcgcacctt tcattctgca ttatttcgag aacgccttgg
      661 tgaaaaaggt tttgaaccga tttacttcct tacctctgac taccttgaag cctctcccta
      721 tgacccgaag cgatacttcg agttgaaggt ttcagcctat gaggcatatt acgaaaagca
      781 tgcattgttg cgcaacatga aatctctaag acgcttcgtg attgtgaccg agacaacaga
      841 tttgcggctg cgagaggaaa tagaaagtat tatttttcaa aaagatcttt ggaaaatcgg
      901 tggatacatt ctttatacaa gtattttgag atgggtgcct gggatggggg attttctgtt
      961 ggggctggaa aatctattgt ataaaactga tgcacataca gaagtgatca gaaatattcc
     1021 attgaactgt gtcctaacgc cgggtatggg aaattatcga tttgaatatt caggacaatt
     1081 ttcgcttgag gcacagcggt tgggaatacc ggttttcagc gctgttacaa attacgataa
     1141 tatcgttaat atgggatttc gtggttttac tccaaaatgc atagcggttt ggagccagca
     1201 aatggcagac gagctcatgc gtttacataa gatgtctggt aagcgcatag aagtgacagg
     1261 tccgattcag tatgaccgtt ttaagcaacc acttcctcgc agccgggaag aatttctcga
     1321 atctattggg cttgacccaa agaaaaaaac catttttatt gcaggtggcg taaacattac
     1381 tcgctatttt gagttattta atctcttttt cgagcagcag acaaatgttt tttcagaacc
     1441 ttgtaatatt attctgcgcc cttatccaca tgtgaaattg ctttcttcac ctggttggaa
     1501 gattttaagg aagttattcc tggacagagg agtttacatc tcaatcccag atgcgattga
     1561 ttcgcatgaa cataggtcgc aagagcttaa gagtgatctc tattctgaag aagaacatga
     1621 tgaattgaat taccttctgc gttacagcga tgtaatggtg aactattttt cgaccatcag
     1681 cctagaagct gctatatgtg atttacctgt gatccatgta ggttacgata tctatacata
     1741 tggacatcgt tttcatgtaa catctgaatt tttacaacga cagactcata atcgaagaaa
     1801 attcaggctt gcagcatcac gtgttgctaa aactgaaaaa gatttggtaa agtatattaa
     1861 tatgtatctt agtgataagt cgttagatcg agatgcacgg tatgaatatg ctatttccga
     1921 gtgtggtgaa ttggatgggc acgcagggag gcgaattgtg caaatgatta agagccgttt
     1981 acaacgccag tgaggaacta atatgaatgt aatgcagaaa cttgtacaag aaattaaaaa
     2041 aagtacacat ctacttcccc cacttcaaag atggatagac catcaggaac aagcggcaga
     2101 tcttgcttac tggcaaatga aacctcgtac ccttcctttg ccacatctcc taaaacaaaa
     2161 tatcttgaag ggttatgcga ttgaatataa attacctgtt ttgattgaga caggtacata
     2221 catgggcgag atggttgaag ctttacgtaa cgtattcaaa accatatatt cgattgagtt
     2281 aagtgataaa tttgcacagc gcgcaaaaaa atattttcgc tattccagta atgtgactat
     2341 tttgcaaggt gacagtggag aggttctaag agaattgatt cctaaaatcg accagccggc
     2401 ccttttttgg ctggatggac attggtctgc agaccaaact gctcgtggga aaaaaagtac
     2461 acctattatg gaggaattgg accatatcct gcatgctaaa gatttggggc atgtaattct
     2521 aatagatgat gctcgtgctt ttggaaaatc tgaagactat ccgaccttgg atacattaag
     2581 tgattttgtc ctttcgctac gtaagaatgt taaaataatt tcggaagatg acattataag
     2641 aattttgcct cagtatgaat aaaccatctt tattaaccat tggtatttta agttggaacc
     2701 gcttgaatta tttacgtgca acgcttgagt ctgctaagcg ttgtattcag tacccgaata
     2761 tccaatggat tgttttggat aattattcaa cagaacctgg tttagctgaa tacttaaaaa
     2821 gccttgcctg gattgacgag ctaatattta tgcaatccag tcatgttgct gctatgaatg
     2881 aaatcgttgc tcgggcaaaa ggggatgtcc ttttattatg gcccgaagat gtgcaatttg
     2941 ttgtggaagg tgattggatg agggattgca ttgaaatatt actaaaagac tattctattg
     3001 gtgcgcttag cttaaatttt caccgccgcc aaacgataga atacatttgg ggaaggcaac
     3061 gctttaataa aaacggtatc ttaagaatgt ggaaagacct gaaacgatat ggtgctaatt
     3121 ttcgccttca aaaaaaaaat atttcttcaa atgggtatcc agtgcgaacg tttggttggc
     3181 aagaagatgg aatcattggt gcaggtatca catctcttgc ccgcacagaa gtatggaaga
     3241 aacttggccc atggaaggca aaacctgctg atgacaatat tatagactca agcggcggtg
     3301 gggaaactga aatgttagaa cgttgggcaa aaacaaaaac gcccttacaa agagctttgc
     3361 caatattgcc agtcagcgcc gatattatta atgacaatat aggctccaag gcaaaagttc
     3421 gcggaaataa gagatatggt gtctatactc cgcctttaca aggggatttt tattacaaaa
     3481 tttatgagca aaaggatgtt gctaatttgc aaaagagaaa tctgcccgtg tcttttgagg
     3541 aatttgttgt gccaattgga tttggcttac cattagactc agagggaaat ttgctgaaaa
     3601 gtagctttaa tgacacaata gttgccgatg ttaattaatt tgcattatct gcaattttat
     3661 gcaccataat tactgaaata gtataattct tgccttggtg tttctgaaaa ataaacataa
     3721 aaaccttcta ccttcagttt tgaaaaggaa aaaatatgca aaatatcaaa agtgtcttag
     3781 gagtgacctt ggggcatgat actagtttcg ctcatgttgt taatgggaaa attgtggcta
     3841 tcatggaagc ggaacgttat tttagacaaa aacgttataa gttacatagt cagacgatgg
     3901 agccaggtaa gcatattagt gggtatcaat atgtttctct ggaagacatg gagttattcc
     3961 tcggtttagt agctaaggaa tggggaaata cttttgatgc gttagctgtt caaaatcaag
     4021 ggcgcataga agaattcaag aattttcaac agattttatc tcgtcttgga tttaagttca
     4081 gttcagttca tcatgttgat catcatctta gccatgctgc gttatctttc ttttcatcac
     4141 cttttaagga agcgcttgtt cttagctatg acggtgaagg taatgatggt cagaccatca
     4201 tctttggcgc caaaggtaat aagcttgagt acttacacaa tgatccaatc cgttttggac
     4261 agagttacaa taatatggga tacattgttg gtatcaagcc tgaagtcgaa ggcactacat
     4321 ctggtaaggt gatgggactt acggcatatg gtgaacctgt ggaagattgg atgccctatg
     4381 cgcgtgaata cattaaaaaa tatataaagg caacccctcg ccaaattgaa ggtctcaaca
     4441 aatatggtaa agcccaccgt attaatgata ttgcggttcg tgatattccc gatcttaagc
     4501 aatatcttgt tcctgaagaa gatagcgatg gtgggttgaa atctgcagtt aagaaaatga
     4561 tagggagaac aaacaccatt ctgcggttga atggtcctga agacaaaacg gctcagaact
     4621 ttgctgctac tgttcaagtt gcatggacta aagctgtaat tgacctgctt aagccctatt
     4681 ttggtaaatt tgagaattta tgtgttgttg gcggatgtgc attaaatggt gttacgaatt
     4741 atgagatcca acaactaggg gttttcaagg cgactcat
//
LOCUS       DAOREF010000102         4763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10220_length_4763_cov_4.334325, whole genome shotgun sequence.
ACCESSION   DAOREF010000102 DAOREF010000000
VERSION     DAOREF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_4763_cov_4.334325"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PKI33_03140"
     CDS             complement(<1..459)
                     /locus_tag="PKI33_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM36026.1"
                     /translation="MTEKKQANIFIVLPAYNAAKTLHRTLDEIPADFRKNIILVDDAS
                     KDNTAELAREAGLTVYVHPKNRGYGGNQKTCYTESLSSGADLVVMLHPDHQYDATVIP
                     HMVQPILDGEADAVFGSRMLGGHPLEGGMPWWKFNANVLLTAVANIIFRRY"
     gene            complement(456..1133)
                     /locus_tag="PKI33_03145"
     CDS             complement(456..1133)
                     /locus_tag="PKI33_03145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36027.1"
                     /translation="MSNLSYQDKLSPWSSHSLIYRLIEKMPAHSKVLDVGTASGMLAR
                     REWARPLRFFGIEAVSEWAEIAAPFYEKMWVCTFDEASEEMLGGYNAIVLGDVLEHMS
                     SPDKALKKLVNLQPSGALFVISVPNIANFWMRLNLLFGRFDYADRGIMDRTHLRFFTR
                     KTIRLLIENAGLEVLSIQVTPIPLELVSPFFLSRVGRVFHYIFAKLTSILPTLLGYQF
                     IVEAKKP"
     gene            complement(1126..2289)
                     /locus_tag="PKI33_03150"
     CDS             complement(1126..2289)
                     /locus_tag="PKI33_03150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNM36028.1"
                     /translation="MNKPFFFIFQPIRTPSFLKLFLFILVLLVCIVFIFIRPDNLDQS
                     DFMLIAWYPGQQLLHTGFVDANYPYPLWTVIVMLPLVVWAPKTAMMLMFVCNMLMLSA
                     SLTLFVLIFKWELSPVLLVLGVSLSVFFLPVLSSLWLGQLTIFSLLILALTLFFYFKE
                     KWGLLGAVLGLSFIKPQVMLLLVGLLLLWAFLKKKWEVLVGFSIVVTILILISLPLVS
                     TPMQLIGGGISSHLGSYILMTSTIWGATMSLGMSWLIPLLISITLLLWLGWIWLPFLV
                     GNKDRPHEFFLFSIAVIVNLVVIPYSWMHNLTLLLLPVGYSLLLISQKSNLVRVIGLI
                     LVFLVMYPLMYLLFILIGIPQVTQAYQIIPALFLLLGMMLLENKNIQTENFYV"
     gene            complement(2291..3889)
                     /locus_tag="PKI33_03155"
     CDS             complement(2291..3889)
                     /locus_tag="PKI33_03155"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNM36029.1"
                     /translation="MPDLSTSLHKQDLGHFRIVAEFWGLELESTDSASALEELCGSLL
                     DLEAVSETLDILPAEARSALNELVSADGKIEWSIFSRKYGEIREMGAGKRDRERPYLK
                     PASTSEILFYRALIAKAFFESDKGSQEFAYIPEDLLEIINEVNVESAPLPSSAEALGR
                     PATPVEKAFETPVNDFVLDDATTYLVALRIGHESKANFKRELPLLLTTAGIIKNDGLL
                     PEAVKKFLEISRKEALDLLYNAWLESTTFDELRLMPSIVCEGEWKSQPQVTREFLMNL
                     INDVPEGKWWSIPAFVKAIKEKYPDYQRPAGDYDSWFIKRASDGQFMRGFAYWDSVDG
                     ALIKYLIQLLHWLGKADLAAPEEGKEPTAFRLVALHPESLRADVKINVVSNGRISVPR
                     TFSRTVRYQLARFCDWDEPKDDDYFYRVTAESLRRATEQGLKAEQLLAMLVKYTKEAV
                     PPAMVKALKRWDANGPEARVESLLVLRVSKPEIMEEMRKSKAAKYFGEVLSPTAIVVK
                     SGAIQKVLAELAELGLLAEISIPQ"
     gene            4000..4506
                     /locus_tag="PKI33_03160"
     CDS             4000..4506
                     /locus_tag="PKI33_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36030.1"
                     /translation="MLILFSVLRFVHIIAGIFWAGGAILMNLIIGPAIGATGDAGKQF
                     VGHLMGKTIFSKFMLASGAITVLAGSILYGIDSNWFSSGWMMSPTGTGFGIGAISGII
                     ALVFGFLIGSTNGKLALLGMQIQGKPTNEQLAALGALRKRQVIVTNGNTIFILISIAL
                     MASARLFG"
     gene            complement(4552..>4763)
                     /locus_tag="PKI33_03165"
     CDS             complement(4552..>4763)
                     /locus_tag="PKI33_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HNM36031.1"
                     /translation="MTGTVSIHGSHIHIAISDEDGITIGAHLVSGCKVYTTAEIVLLE
                     LDDVVYKRELFENDSGYEELVVYKK"
BASE COUNT         1525 a         1003 c          951 g         1284 t
ORIGIN      
        1 gtagcgacgg aagataatat ttgccactgc tgtgagaaga acattagcat tgaacttcca
       61 ccatggcatg ccaccttcaa gcggatgacc acccaacata cgtgaaccaa agacagcatc
      121 cgcttcaccg tctaaaatcg gttgaaccat atgtggaatt actgtagcat catattggtg
      181 atctgggtgt aacattacaa ccaagtctgc accagaagat agagattcag tgtagcaagt
      241 cttttggttg ccaccataac cacgattttt ggggtgaaca tagacagtca accctgcttc
      301 acgtgcaagc tctgctgtat tatcttttga agcatcgtcg acaaggatta tattcttacg
      361 gaaatctgca ggaatctcat ccaaggtacg gtgtaaggtt tttgccgcat tatatgcagg
      421 caatacaatg aaaatattag cttgtttttt ttcagtcatg gtttcttcgc ttcaacaatg
      481 aattgataac ccaatagcgt tgggagtata gaagttaatt tggcgaaaat gtagtgaaat
      541 actctaccta ccctagataa aaagaaagga gacactaact caaggggaat aggagtcacc
      601 tgaatagaca gcacctccag cccagcattt tctattaaca accggatggt tttgcgggta
      661 aagaaacgta aatgagtccg atccataatt ccacggtctg catagtcgaa acgtccaaac
      721 aacaaattaa gtctcatcca aaaattggca atattaggga ctgaaataac aaatagagca
      781 cctgagggtt gtaagttaac caacttcttc aaagctttgt caggtgaaga catatgttca
      841 aggacatcgc ctaaaacaat agcattatat cctcctagca tctcttccga agcttcatca
      901 aaggtgcaca cccacatttt ttcataaaaa ggagcagcaa tctccgccca ttctgatact
      961 gcctcaatac caaaaaaacg tagagggcga gcccactctc gacgtgcaag cattccagac
     1021 gccgtaccca catccaacac tttgctatgt gcaggcattt tttcaattaa cctataaatc
     1081 agtgaatggc ttgaccatgg acttagttta tcttgatagg acagattaga cataaaagtt
     1141 ctctgtttgt atatttttat tttccaacaa catcattcct aataaaagaa acaaagcggg
     1201 aataatttga taagcctgag ttacttgcgg tatgcctatc aaaataaaaa gtaaatacat
     1261 aagcggatac attaccaaaa aaacaagtat caacccaatc acacgcacta aattgctttt
     1321 ttgggagata agtaaaaggc tatagccaac aggaagtaac aacaaagtaa gattatgcat
     1381 ccaactgtaa gggataacaa ctaggttaac tataacagca attgaaaaca agaaaaactc
     1441 atggggtcta tccttatttc ctacaagaaa aggtagccat atccacccaa gccacaataa
     1501 aagggttatt gaaatgagta gcggaataag ccaagacata cccaaactca ttgttgcacc
     1561 ccaaattgta ctagtcatta aaatataact tccaagatgg ctacttatcc cgccaccgat
     1621 caattgcatt ggagtagaga caagcggtag tgaaatcaga atcaatatag ttacaacgat
     1681 actaaagccc accaaaacct cccatttctt ttttagaaat gcccataaca aaagcaagcc
     1741 aacaagcaat agcataactt gaggcttaat aaaagataga ccaagaacag ccccaagcaa
     1801 gccccatttt tctttaaaat aaaaaaatag agtcaaagca agaataagca aagagaaaat
     1861 agttaattgc cccaaccata aactacttaa tacaggcaga aaaaatacag atagagaaac
     1921 acctagcacc agcaacactg gacttaattc ccacttaaaa atcaaaacaa acaaagtaag
     1981 actagcagaa agcattagca tattgcagac aaacatcagc atcatagctg tttttggcgc
     2041 ccaaactaca agaggcagca tgacaataac agtccataat ggataagggt agttcgcatc
     2101 cacaaagcct gtatggagta attgttgacc gggataccat gcgattaaca taaaatcgct
     2161 ctgatctaaa ttatcaggac gtataaaaat aaaaactata caaaccaaga gaactaaaat
     2221 aaaaagaaaa agctttagaa aacttggtgt acgtataggt tgaaagataa agaaaaaagg
     2281 tttattcatc ttattgtgga atagatattt ctgccaacaa acccaactcg gcaagttctg
     2341 ccaacacttt ttggatagct ccgcttttga ccacaatggc tgttggactt aaaacttcgc
     2401 caaaatattt tgccgccttc gacttacgca tctcttccat aatctcgggc ttgctgactc
     2461 gcaacacaag cagactctcc acccgggctt caggtccgtt tgcatcccaa cgcttgagcg
     2521 ccttgaccat tgctggagga accgcttctt ttgtatactt aaccagcatc gccaataatt
     2581 gttccgcttt caacccttgt tctgtggcac gccgtaatga ctctgctgtg acgcggtaaa
     2641 aatagtcatc gtctttgggt tcatcccaat cgcaaaaacg cgccagttga taacgaaccg
     2701 tccgtgagaa ggtacgtgga acagaaatcc tgccgtttga gacaacattg attttcacat
     2761 ctgctcttag gctttcaggg tgtaaagcaa caagacgaaa tgcggttggt tcttttcctt
     2821 cttcaggcgc agctagatcc gctttaccga gccaatgcaa tagttgaatc aaatatttta
     2881 ttaaggcacc atcaacagag tcccaataag caaacccgcg catgaactgt ccatcagatg
     2941 cgcgtttgat aaaccacgag tcataatcac cagcagggcg ctgataatct ggatatttct
     3001 ctttgatggc ttttacaaaa gcgggaatac tccaccattt gccctctggc acatcgttga
     3061 tgagattcat taaaaattca cgtgttactt gcggttggct tttccactcc ccttcacaaa
     3121 caatactagg catcaagcgc aattcatcaa aggtcgtgga ttcaagccag gcattgtata
     3181 gaaggtctaa tgcttctttc cgtgagattt caaggaattt cttcacagct tctggaagaa
     3241 gcccgtcgtt cttgattatg cctgcggttg ttaataacag aggcaattca cgtttgaaat
     3301 ttgctttgct ctcatgccca atgcgcaagg caacaagata ggtagttgca tcatccagaa
     3361 caaaatcgtt tacgggggtt tcaaaagctt tttcaacagg agtagcaggg cgacccaatg
     3421 cctctgctga agaaggtaat ggcgcacttt ctacatttac ttcattgata atctctaaga
     3481 ggtcttcagg gatgtaggca aattcttgtg agcctttatc tgactcaaaa aaagctttgg
     3541 ctatcaatgc acgataaaaa agaatttcag aagtagatgc aggcttgaga taagggcgtt
     3601 cacggtctcg cttgccggcg cccatttcgc gaatctctcc atatttgcga gaaaagatac
     3661 tccattcaat ctttccatct gcggatacaa gctcgttgag cgcagagcgg gcttcggctg
     3721 ggagaatatc aagagtttca gaaacagctt caaggtccag taaagagccg cagagttctt
     3781 ccaaggcaga agctgaatca gttgattcaa gctccaagcc ccagaactct gcaacgatac
     3841 ggaagtgtcc caaatcttgt ttgtgtaagg aggtggacaa atcgggcatg gggttattct
     3901 acatgaagga gcaagacttt agaaggattt tgttttgtga attaaaatgg ataaaatata
     3961 tcttattatt agatttgata aattttaaag gagagtacca tgttaatttt gttttctgtt
     4021 ttacgatttg tacatattat tgcaggaatt ttctgggcag gtggcgctat tttgatgaac
     4081 ttaattattg gacctgccat tggcgcaact ggagatgcgg gcaaacaatt tgttggtcac
     4141 ttaatgggaa aaactatatt ttcgaaattt atgcttgctt cgggagcaat aaccgttttg
     4201 gctggaagta ttctatacgg tattgattca aattggttta gctctggatg gatgatgagc
     4261 ccgacaggca ctggcttcgg aatcggtgct atttctggga ttatagcgtt agtctttggg
     4321 ttcttaatcg gaagtacaaa tggaaaatta gctttacttg gcatgcagat tcaaggtaaa
     4381 cctacgaatg agcaattagc ggctttaggt gcattgcgca aacgccaggt tattgtaaca
     4441 aacggaaaca caattttcat tcttatatcg attgctttga tggcgtctgc acgattattt
     4501 ggataaatgc gttgcaataa aagacagaca cccatcaagg gtgtctgtct tttatttttt
     4561 atacacgacc aattcttcgt agcccgagtc attttcgaat aattcgcgct tataaactac
     4621 atcatctaat tcgagtaaca caatctctgc ggtggtgtag accttacagc cgcttacgag
     4681 gtgagcacca atggtaatgc cgtcttcatc agaaatggcg atatgtatgt gtgaaccatg
     4741 aatcgatacg gtgccggtca tgg
//
LOCUS       DAOREF010000103         4757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10243_length_4757_cov_5.321778, whole genome shotgun sequence.
ACCESSION   DAOREF010000103 DAOREF010000000
VERSION     DAOREF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10243_length_4757_cov_5.321778"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..2439
                     /locus_tag="PKI33_03170"
     CDS             25..2439
                     /locus_tag="PKI33_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36032.1"
                     /translation="MNLLTVLTAILFLCIVVLLVYYFFHQKQKNRYSKSTTLVLKDAK
                     KTIQASVSVDVPEEMVVHVTLKLVDKKGGKTTSVDGTLSHTTPKNKPKKSQVSIKSVK
                     NAKTTNKFNFQNILNSFQKIDLIQKIKNSNTGLILLLASILVYVLVISIGIERFPIYF
                     FTDEAIHMNFISDFIRDGYRNYNREFLPTFFIASGWVNGTSVYVQWLPYLLFGKSIAV
                     TRLVSAFITLISVISLGLLLKQVFKIKYYWAGILILLTTPAWFLHARTAFEYAEVASF
                     YMIFLYFYSRYRDGHLQSLYFAILSGALAFYTHGLGQILMSITGTALFFADIKYHIHP
                     DRRKTVLWALVFGIILLLPFARYYLAHPNESFEQVQRRNSYWFNVNWTTSQKILEFIK
                     QYTYGLNPLYWYFHNSVDLDRHVMKGYGHGLLVTLPFALIGFTKVVRNLHHFSYRTLF
                     IAFLAAPLPASVVAIGMPRMLWMSIPLALMTTIGLSLSLEKLDEYWIKISKYTPWILF
                     TVLTSLSFFMLRDALVNGPLWFKDYGLFGMQYGAKQVFEETILPELKSNPNIMFSVSP
                     SWANGTDEFADFFIPAELRPRVFFGQPIDWINNLSSINQNLHFILPYNEYNNLIQDPK
                     FKAITVYKVINYPDGTPGFYVVSLTPADNITSILNAEKIKNITPVDEMLQLAGQEVHV
                     AHSPLGAGSLTDVFDNDPNTLVRVLEANPFIFDISPTTPIDTQSIIIQTGTQPNFTIK
                     VSLYAPGENDPVTYTNTYKDLPPDPTVTINFDKGPQKSARIYIEIEDNTSGETSQIHV
                     RTINIK"
     gene            2697..>3481
                     /locus_tag="PKI33_03175"
     CDS             2697..>3481
                     /locus_tag="PKI33_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36033.1"
                     /translation="MSFFNLFNKSPKKEQKQDKISTVKPWMENPLASKELQKKRYDAA
                     MDFLEFFQGKMPLKGGNPHAGTVLSVAAQLAGTSLFRAVHGQKEFPAGNVILSEEINQ
                     AYPQLLNLFALYCKQNGMDVMSKPLVTSFPEKDKPLMELDQVQAEYQEEFTKIMQKHG
                     LDHLESARAGMVISSILFTYHCIRSKDIDPYIATGIVAMGVIQGAKTAPVPIGARPNT
                     EVSSGTSRRFLVGDPDVVRKEGMATGATYLDVNPMILKALQEKG"
     assembly_gap    3482..3581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3582..4555)
                     /locus_tag="PKI33_03180"
     CDS             complement(<3582..4555)
                     /locus_tag="PKI33_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36034.1"
                     /translation="MKNNNSTILIVDDDAAGREVLVSILESDGYELILAENGFQAIEL
                     ANAIHPDIILLDIMMPGMDGFEVCRRIRSEKDIAEVPILFLSALDDRQSLLSGLEAGA
                     DDFINKPPDRYELRARLLGISRLNRYRKLLDERQNLEEAHQQLLLAYDKTIEGWSRAM
                     DLRDKETEGHTQRVTQTTLKLAKFFGIQGEELIHIQRGALLHDMGKLGIPDAILLKPD
                     KLDDDEWKLMCQHPQMAYDMLYPIEYLRPALDIPYCHHEKWDGTGYPRGLKGEEIPLS
                     ARIFALADVWDALTSNRPYREAWSEQKTLAYIRQQSGKQFEPRVVEAFT"
BASE COUNT         1442 a          983 c          826 g         1406 t
ORIGIN      
        1 aacaagcata ggctaacggc taacatgaat ctcctaaccg ttttaaccgc tattcttttc
       61 ttatgtatcg ttgttctttt agtgtattat ttttttcatc aaaaacaaaa aaacaggtat
      121 tccaaatcta caaccttggt tcttaaagat gcaaaaaaaa cgatacaagc aagtgtgtct
      181 gttgatgtac cagaagaaat ggtagttcac gttacattaa aacttgtaga caaaaaaggt
      241 ggaaagacaa cttctgtcga tggtacactt tcgcatacaa cccccaaaaa taaacctaaa
      301 aaatctcagg tatctattaa gtccgttaaa aacgccaaaa cgacgaataa atttaacttt
      361 cagaatatat taaacagttt tcaaaaaata gacttaatcc aaaagataaa aaactcaaat
      421 acaggattga ttctcctact tgcatcaatt cttgtttatg tacttgtcat aagcatcggg
      481 atagagcgtt tccctatcta ctttttcact gatgaagcca ttcacatgaa tttcatctca
      541 gattttattc gtgatggcta cagaaactac aaccgcgagt tcttaccgac attttttata
      601 gcaagtgggt gggttaacgg aaccagcgtt tatgtacaat ggcttcctta tttacttttt
      661 ggaaaatcaa ttgctgttac ccgtctagta tccgctttta ttacattaat cagtgtaatc
      721 tctcttgggc tgctattaaa acaagtattt aagatcaaat attactgggc aggaatactt
      781 atacttctaa ccaccccagc atggttctta cacgcccgca cagcttttga atatgccgaa
      841 gtagcatcct tttatatgat cttcctatat ttttatagcc gctaccgaga tggacattta
      901 caatctctat attttgcgat cctatcaggt gcattagcct tttatactca tgggctcggg
      961 cagatattaa tgtcaattac tggaacagct ttgttttttg ctgacatcaa gtatcacatt
     1021 catccggatc gcagaaaaac tgttttgtgg gcattggtgt ttgggatcat tctgctccta
     1081 ccttttgctc gttattattt agcgcatcct aatgagtctt ttgagcaagt tcaaagaagg
     1141 aattcttatt ggttcaatgt caactggact acttcgcaaa aaatcttgga gtttatcaag
     1201 caatatactt acggattaaa tccactttac tggtacttcc acaactctgt agacttagac
     1261 cgccatgtca tgaaaggata tgggcatggt ctgctagtca cgctaccctt tgcattgata
     1321 ggtttcacaa aagtggttag aaatttgcac catttttcat atcgtaccct tttcattgcc
     1381 ttccttgccg ccccgcttcc agcatctgtt gtagctattg ggatgccacg catgctttgg
     1441 atgtcaattc cgcttgcact tatgacaacg ataggattat ctttaagcct tgaaaagtta
     1501 gatgaatatt ggataaaaat ttcaaaatat accccctgga tactattcac cgtactaaca
     1561 tccttaagct tttttatgct acgggatgct cttgtaaatg gacctctttg gtttaaggat
     1621 tacggattat ttgggatgca atatggcgca aagcaagttt ttgaagaaac gattttgcct
     1681 gaattaaaga gcaatcccaa tatcatgttt tctgtttcgc catcatgggc aaatggcaca
     1741 gatgaatttg cagatttctt tatccctgca gaattaagac cacgtgtttt ttttggtcag
     1801 ccaattgact ggattaataa tctatcctct ataaatcaaa acctacattt tatcctcccg
     1861 tataatgaat ataataatct aatacaagat ccaaaattta aggctataac cgtttataaa
     1921 gtgatcaatt accctgacgg cactcctggt ttttatgtag ttagtcttac gccagccgat
     1981 aatattacaa gcattctaaa tgctgaaaaa ataaaaaata tcacccctgt cgatgagatg
     2041 ctacagttgg ctgggcagga agtacatgtt gcccattctc cattaggagc tggttcctta
     2101 actgatgtat ttgataatga tccaaatact ctggttagag tcttagaagc aaatcctttt
     2161 atatttgata tttctccaac tacaccaatt gatactcaaa gtattattat ccaaacaggg
     2221 actcagccta atttcaccat taaggtttct ttatatgcgc ctggtgaaaa tgaccctgtg
     2281 acctatacaa atacatacaa agaccttcca ccagatccaa cagtaaccat taatttcgat
     2341 aaaggtcctc aaaaatcagc tcgcatttat attgaaatag aagataacac atctggtgaa
     2401 acctctcaga ttcacgttcg tactatcaat attaaataag tcttgagtat tatcaatatc
     2461 aaattctacc tgctaaaatg tgcaagaagc ttctcgcaca ttttagcagg tgattaattg
     2521 gtaataagat gaaaaagtat gctgtgtatc ctaattcata tcaaatcaaa tagggcatat
     2581 cagattcacc aataaaaatc tactatcttt tagggtgtga aacagaggca cccaatattt
     2641 ttgtaaactt ctattcttaa actatcaggt aagatattga aataaaggag ggcacgatgt
     2701 cattttttaa cctattcaat aaatcgccca aaaaagaaca aaaacaagac aaaataagta
     2761 ctgttaagcc gtggatggaa aacccacttg catcaaaaga actgcaaaag aaaagatatg
     2821 atgctgccat ggattttctt gagtttttcc aagggaaaat gcccctcaaa ggagggaatc
     2881 ctcatgctgg aacagtcctc tcggtagctg ctcaacttgc tggcactagt ctatttagag
     2941 ccgtacatgg acaaaaagaa tttccggctg ggaatgtcat cttgtcagaa gagatcaatc
     3001 aggcataccc acagcttctt aatctgtttg ctctttactg taaacaaaat ggtatggatg
     3061 taatgtccaa acctcttgta acaagttttc cagaaaagga caagcctctt atggaattag
     3121 atcaagtgca ggcagaatat caagaagagt ttaccaagat catgcaaaaa catggattag
     3181 atcatctgga aagtgcgcga gcagggatgg tgatcagctc aatcctcttt acatatcatt
     3241 gcattcgttc caaggatatc gatccctata tagcaacagg tattgtcgct atgggagtga
     3301 tccaaggtgc caaaactgca cctgtaccta ttggagctcg accaaataca gaggtgagca
     3361 gtggcacttc caggcgtttt cttgtaggag atccagatgt ggtgcgtaaa gaagggatgg
     3421 caacaggggc aacctatttg gacgtaaacc caatgatctt gaaagcttta caagaaaagg
     3481 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtaaaagcc tctacaaccc
     3601 gcggctcaaa ttgctttcca gactgttgcc gaatataagc taatgttttt tgctcgctcc
     3661 atgcttcacg gtaggggcgg tttgatgtca gtgcatccca aacatcggct aaggcaaaga
     3721 tgcgagcgga aagtgggatc tcctcacctt tcaaaccacg tggatacccc gtaccatccc
     3781 atttttcatg gtgacaatat gggatatcaa gagcagggcg aagatattcg atcgggtaaa
     3841 gcatatcgta tgccatttgt ggatgctgac acatcaactt ccattcatca tcatccagtt
     3901 tgtctggctt taagagaatt gcatctggaa tccccaattt acccatatca tgtaacaaag
     3961 ctccacgttg aatatgaatt agttcttctc cttgaatacc gaagaatttg gcaagcttca
     4021 gagtggtttg tgtaacacgt tgagtatgcc cctccgtttc cttatcgcgc aaatccatgg
     4081 cacgagacca gccttcgatg gttttatcat aggctaaaag aagttgttga tgcgcttctt
     4141 caaggttttg gcgttcatct aataattttc ggtaacgatt caatcgagaa atgccaagca
     4201 accttgctcg taactcatag cgatctggcg gcttgttaat aaaatcatct gcccctgctt
     4261 ctaacccact taacagagat tggcgatcat ccaatgcact aagaaataaa atcggaactt
     4321 cagcaatatc tttttcgctt cggatccttc gacaaacttc aaaaccatcc atacctggca
     4381 tcataatatc caaaaggata atatcgggat gaatagcatt tgcaagctca atagcttgaa
     4441 atccattctc tgctaaaatc aactcataac catctgattc aagaatagat actaaaactt
     4501 ctcttccagc agcatcatca tctacgatca aaatcgtact attattattt ttcaccataa
     4561 cctccaatac tctataacct tctaggcatt tttggcaatt ggaatagaca gacgaaccac
     4621 aacccccggt ttatcttcac gattcaacat ctggcttgtc ccaccagcgc cccacacaat
     4681 agcattaact gtagataacc ccaaccccat gcctggcatt tcgcctgtga aatctttttc
     4741 gccttgataa tatggca
//
LOCUS       DAOREF010000104         4733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10313_length_4733_cov_4.549808, whole genome shotgun sequence.
ACCESSION   DAOREF010000104 DAOREF010000000
VERSION     DAOREF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10313_length_4733_cov_4.549808"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..991
                     /gene="panB"
                     /locus_tag="PKI33_03185"
     CDS             113..991
                     /gene="panB"
                     /locus_tag="PKI33_03185"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNM36035.1"
                     /translation="MSTTTISTNTSQRKKVTTLTFRQKKERGEPITMLTAYDYPTAVA
                     IDKAGIDSILVGDSLAMVVLGYENTLPVTMDEMLHHARAVSRGAKAPLLVGDMPFMSY
                     QVSVEDAVRNAGRFLQQGGMDAVKLEGGRERADAVRAIVNAGIPVMGHLGLTPQSVNQ
                     LGGFRAQGKTASAAKRLLEDAQILEEAGAFSLVLESVPARLAEYISKHISIPTIGIGA
                     GTGCDGQVLVTHDLLGLFERFTPKFVRQYAKLHETMNKAFTEYLNDVNTKQFPAAEHT
                     VEMTDEEWAAFQKEVK"
     gene            988..1884
                     /locus_tag="PKI33_03190"
     CDS             988..1884
                     /locus_tag="PKI33_03190"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNM36036.1"
                     /translation="MSENILLIGTGALSTLFAARLSEAGHHVSMLGTWKDGIQALNQN
                     GARVSESNGKEHAFQVHATDEPSEVRNVKHAIVLVKSWQTKRVAEKLKGILSPDGIVL
                     TLQNGLGNRETLARDLGAERVALGVTTTGATLLGPGHVKVGGEGILSLEQNQALGPLE
                     AALRSSNFNLQIVQNAQSLVWGKLIINAAINPLTALLQVPNGELLNHPWARKAMNALA
                     REAAEVAQAEQVNLPFNNPIEAVEEVARKTAQNLSSMFQDVRRGAPTEIDAICGAVTK
                     RGEMHGISTPYNRSCWQLVKAM"
     gene            2032..2934
                     /locus_tag="PKI33_03195"
     CDS             2032..2934
                     /locus_tag="PKI33_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNM36037.1"
                     /translation="MRKLFLLLLMGAFLSFPISASAQSDVSLSFATVQLWPEYDDPSM
                     LVIVDFQVAAATSLPATLTFRIPAEANLIAVASDTGNGQFLDHPYTGPNPEGEYQTFS
                     MVLEQNTPYRFEYYQPLSYNGDTRIFSYLWDSEYAVENFQYLFLEPLDVTDIALDPKY
                     TAKETFNGLNYYDGAPVQLAAGEQYSMKVSYTKTTDTLVSQAQSVQIAEPVNEDTPGR
                     VSLANSLPYIIGGLGIIMIAGGLMYYFQWGRHSAGGGKHRRRSRTNTESDATSIYCPQ
                     CGARAKPSDRFCRTCGARLRHDEA"
     gene            2989..4449
                     /locus_tag="PKI33_03200"
     CDS             2989..4449
                     /locus_tag="PKI33_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNM36038.1"
                     /translation="MENLSVTVIYFFYGLAFFSMGLLVAMEGGRSTDARLRMALRPLA
                     GFGLVHAAHEWLEMFRAMGHLNAFFTGIYPLLALSILAFSFLSLAAFGAYLVLGSEST
                     WRVSLIIPLGLEAIWVYGLLIFKSKYSLDEIYTVADVWTRYSIAIPAGLLAAAGLVVQ
                     QRAFRRAGLVSFGRDSLWASVAFIWYSLVGQLFTEPSPLPPSTFLNSALFLNLFGFSI
                     QLLRAVTAMFASLFVTRFLRAFQVESDSKIAGLQAAQLHESQQREKLRAQLFRSIVGA
                     QESERQRIARDLHDETGQSLTAIGMGLRGLANETDSKKRLVTLTQLQSLTSDSIRELQ
                     RIISDLRPAHLDDLGLSATLRWYASRVHELTSLHVRVDIEGDEPALDDAVKITIFRIM
                     QEALNNVIKHAQATTVNVKIHYLQKEVRIHVFDNGVGFEMKAVQNRIGRVSLGLAGME
                     ERAALLGGGVTVSSRPGYGTEVEAVVPLHLIDQEEK"
     gene            4446..>4733
                     /locus_tag="PKI33_03205"
     CDS             4446..>4733
                     /locus_tag="PKI33_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36039.1"
                     /translation="MTIRLLLVDDHAVVRTGLKMLLNGQEDLEIVGEAGSAAEALTET
                     ERVQPTVILMDIGLPDKTGIEATREIKAKFANVNIVALTIHEDEEYFFQMLN"
BASE COUNT         1202 a         1138 c         1197 g         1196 t
ORIGIN      
        1 ttaccgcgtg tatctttatg aacgcgacgg aaaaaccaaa atcaaccgaa aatgtgtgca
       61 aaatagggtg tcagcacact aacgcggact ctacctttat cggagggtac ctttgtcaac
      121 aactaccatt tctaccaaca cgtcacaacg caaaaaagtg acgactctca cgttcaggca
      181 aaagaaagaa cgcggcgagc cgattacgat gttaacggca tatgactatc ccaccgcagt
      241 ggcgatagac aaagccggga ttgattccat tctggtgggc gattcacttg ccatggtggt
      301 gcttggttat gaaaatacct tgcccgtcac catggacgaa atgctgcatc atgcccgtgc
      361 tgtatcccgc ggagcaaaag ccccattgct tgttggcgat atgccattta tgtcgtatca
      421 agtctcagtg gaagatgctg tgcgcaacgc tgggcgtttc ttacagcaag gtggaatgga
      481 cgcggtcaag cttgaaggcg ggcgtgaacg agctgatgca gtgcgcgcca ttgtcaatgc
      541 aggcattcct gtgatgggtc acctcgggct tacgccgcaa tcggtgaatc aacttggtgg
      601 gttccgtgca cagggcaaaa cagcgtctgc ggctaaacgt ctacttgaag atgcgcagat
      661 tcttgaagaa gctggggcgt tcagcctcgt gctcgagtct gtgcccgcaa gattggcgga
      721 atatatttca aaacacattt ccattccaac cattggtatt ggcgcaggga caggctgtga
      781 tggacaagtg cttgtcaccc acgacttact gggtctattt gaacgcttta caccgaaatt
      841 tgttcgccaa tacgcaaagt tgcacgaaac aatgaacaag gctttcaccg aatatctcaa
      901 cgatgtgaac accaaacaat ttccggctgc cgaacatacc gttgaaatga cagatgaaga
      961 atgggctgct tttcaaaagg aagtgaaatg agcgagaata ttttacttat tggtacaggc
     1021 gcgctctcta cattgtttgc agcccgtttg agcgaagctg ggcatcatgt atccatgttg
     1081 ggcacatgga aagatggcat tcaagctctt aatcaaaacg gcgctcgtgt atcagaatca
     1141 aacggaaaag aacacgcctt ccaagtccat gctaccgatg aacctagtga agttcgaaat
     1201 gttaaacacg ccattgtctt ggtcaaatct tggcagacaa aacgtgtggc agaaaaactt
     1261 aaggggattc tctcacccga tggaattgtg ttgaccctgc aaaacgggct tggaaatcgc
     1321 gaaacgctcg cacgggacct tggggctgag cgcgttgccc taggcgttac caccactgga
     1381 gccaccctgc ttggacctgg gcacgtaaaa gttggcggcg agggcatcct ctcactggaa
     1441 cagaatcagg ctctcggtcc gcttgaggcg gctcttcggt catccaactt taatttgcag
     1501 atcgttcaaa atgcgcaatc cttggtgtgg ggcaaactca tcatcaatgc agctattaac
     1561 ccgttgactg cgttgttgca agtgccaaac ggtgagttgt tgaatcatcc ttgggcacgc
     1621 aaggcgatga atgctctggc gcgtgaagcc gcggaagtgg cacaagcgga acaggtgaat
     1681 ttgccattca acaacccgat cgaagcggtg gaagaagtgg ctcgcaaaac ggcgcaaaat
     1741 ctttcatcaa tgtttcaaga tgtacggcgc ggtgctccga cagagatcga tgccatttgt
     1801 ggtgcggtga ccaaacgcgg agaaatgcac ggaattagca cgccatacaa tcgttcttgt
     1861 tggcaattgg taaaagcaat gtaattaagt acgggcggcg catgcgccgc ccgtaccgat
     1921 ataaaaaggg tgacatttat ctatattctg catgatttcg tttctgccct caaaaaatgc
     1981 gacatggtaa gatacatata gaagtaaaac atttatcgtt aggaggcgtt catgcgcaaa
     2041 ctattcttac tccttcttat gggagccttc ctctcgttcc cgatttccgc atctgcacaa
     2101 agcgatgtgt cgctttcatt tgccactgtg caattatggc ccgaatatga tgaccccagc
     2161 atgctggtga tcgtagattt tcaagtagcg gcagctacaa gtttgcctgc tacactcacc
     2221 ttccgtattc ctgcagaagc caacctcatc gctgtcgctt cagatacagg caacggacaa
     2281 ttcctcgatc acccatacac aggacccaac cccgaaggtg aatatcaaac ctttagtatg
     2341 gttcttgagc aaaatacccc ctatcgtttt gaatactatc aaccgctttc atacaacggg
     2401 gatactcgca ttttctctta tctttgggat agcgaatatg cggttgagaa ttttcaatac
     2461 cttttccttg aacctttaga tgtcaccgat attgcgctcg acccgaagta cactgccaaa
     2521 gagacgttca acggattgaa ctattatgac ggtgcgcctg ttcaacttgc ggcgggtgaa
     2581 caatattcga tgaaagtaag ttacaccaaa actacagata ctttagtctc gcaggcacag
     2641 tcggtgcaaa tcgcagaacc ggtgaacgaa gatacgccgg gtcgcgtttc acttgccaat
     2701 agtttgcctt acatcattgg cggacttggc atcattatga tcgcaggcgg cttgatgtat
     2761 tacttccaat ggggacgcca ctctgcaggc ggtggtaaac atcggcgccg ttcacgaacg
     2821 aatacagaaa gcgatgccac cagcatctac tgtccacaat gtggggcacg tgccaagcct
     2881 agtgacaggt tctgtagaac ctgtggagca aggctaaggc acgacgaagc ataacgagga
     2941 gagaccctaa aggcgaacaa agcgcctttg gggtcttttt atgtaagcat ggaaaatctg
     3001 tctgttacag tcatctattt cttttatggg cttgcctttt tcagcatggg cttgttggtt
     3061 gccatggaag gagggcgctc tacagacgcg cgcttgcgta tggcgttacg tccactagca
     3121 ggctttggtc ttgtacatgc cgcccatgaa tggctggaaa tgttcagagc catggggcat
     3181 ttgaatgcat tttttacagg catttatcca ctgctggcat tatccatttt ggctttttcg
     3241 tttttatcac tagcagcatt cggcgcttat ctcgtccttg gcagcgaatc gacttggcgt
     3301 gtgagtctca tcattccact ggggctcgaa gccatctggg tttatggctt gcttattttc
     3361 aaaagtaaat attcgctaga cgagatttac accgtcgccg atgtgtggac gcgatactcc
     3421 attgccattc cagcagggtt gttggcagcg gctgggttgg tggtgcaaca acgtgcgttc
     3481 cgcagagccg gtttagtaag cttcgggcgc gatagtttat gggcatcagt ggcgttcatt
     3541 tggtatagct tagtaggtca attatttaca gagccaagcc cattaccacc ttcaactttc
     3601 ttaaactcgg cactgttctt aaatctattc gggttttcca ttcagctact acgcgcagtt
     3661 actgccatgt tcgcttcctt gttcgtaacg cgttttttgc gcgcctttca agtggaaagc
     3721 gacagcaaga tcgcaggact acaagccgca caattacacg agtcacaaca acgcgaaaaa
     3781 ctgcgcgctc aactgtttcg cagcatcgtg ggtgcccaag agtctgaacg tcaacgcatt
     3841 gcgcgcgatc tgcacgatga aaccggtcag tccttaactg ccattggcat ggggttgcgc
     3901 ggtcttgcga atgagactga ctcaaaaaag cgcctcgtca ctctgaccca actccaatct
     3961 cttacctcag actccatccg tgaattgcaa cgcatcattt cggacctcag gcctgcacac
     4021 ctcgatgacc tgggtctttc tgccacgttg agatggtacg cttcgcgcgt gcacgagctg
     4081 acatccctgc atgtacgcgt ggacatcgaa ggcgatgagc ccgctcttga cgacgccgtg
     4141 aaaatcacca ttttccgcat tatgcaagaa gcgttgaata acgtcatcaa acatgctcaa
     4201 gccacaacgg taaacgtcaa gatccattat ttgcaaaagg aagttcgcat tcatgtgttt
     4261 gataatggcg tgggatttga aatgaaagcc gtacagaacc gcattggacg tgtttcttta
     4321 ggcttggcag gcatggaaga acgtgctgcg ttactcggtg gcggtgtgac cgtctcttcg
     4381 cgcccaggct atgggacgga agttgaggcg gttgttccgt tacatttgat agatcaggag
     4441 gagaaatgac gattcgattg ttattggtgg atgaccatgc cgttgtccgc acagggttga
     4501 agatgttgct caacgggcag gaagaccttg agattgttgg ggaagcgggg tctgcggcag
     4561 aggcattaac cgaaaccgag cgcgttcaac cgacggtcat tctgatggat attggtttgc
     4621 cagataaaac cggcatcgaa gccactcgtg agatcaaggc aaagtttgcg aatgtaaata
     4681 tcgttgcgct gaccattcac gaggatgaag aatatttctt ccaaatgttg aat
//
LOCUS       DAOREF010000105         4701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10431_length_4701_cov_3.963625, whole genome shotgun sequence.
ACCESSION   DAOREF010000105 DAOREF010000000
VERSION     DAOREF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10431_length_4701_cov_3.963625"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..708
                     /locus_tag="PKI33_03210"
     CDS             94..708
                     /locus_tag="PKI33_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM36040.1"
                     /translation="MKKEITITSIRVADLLAEGERMPVYVHVIDHPNGRVLVDTGFTE
                     LHPAIADLDPRIFPLNEQNVDLASIDIVVNTHLHFDHCGGNHLFPGKPIYVQQAELDD
                     ALTKDDYTIREWVDAPGVKYMPVDGEFELLPGLRLVPAPGHTRGMQMVVVETGERPII
                     IVGGDVAVWFGELDEPKTEGQLKVHTLNPQLVWLTHEHEPWRPK"
     gene            768..1880
                     /locus_tag="PKI33_03215"
     CDS             768..1880
                     /locus_tag="PKI33_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNM36041.1"
                     /translation="MAYSIKYPRKVFIRSTFRFTIRSLFPLLAQTKITGSKNFPQNGP
                     LILVGNHTGAMEVVLMGLYSPKPVEFMGAMEMPWNGWMGKMIDLYGLIPVHRGYTSNT
                     TMKMGIDVLRQGGILGIFPEGGFWEPGKQKAQSGVAWLSYAAQAPILPIGFGDTRGKM
                     AEIFQLKRPAFEMNIGDVIPPVQLEKSAHKKEELQQAADRIMDAVWALVPEDERKRKE
                     SQPENELFTFDISIFDQHGTPIPIPADLQLTDGSWISRFAHRPNLIDSIRDYIFIPVQ
                     VLKELHRKPPAEEIYLAAHSMLEYVERDNPQYFNYRYGYKDGEVFQQSFRQLRDLMKW
                     AMENQYQVAAEARYEYTDPSTGERRVLHVPQELEQW"
     gene            1923..3278
                     /locus_tag="PKI33_03220"
     CDS             1923..3278
                     /locus_tag="PKI33_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36042.1"
                     /translation="MSNSERLSTRFKMLFSTGDLSTSIPLAILMFFQLYFLTDVARLR
                     PDYAGWAIALPRLWDAINDPLFGLLSDRLRTRWGRRRALLLFGSVPLGLSFILMWIVP
                     PFGQLGLAIYYALTFIVFDTVFTVVHVGYNSLTPELTSDYDERSSLNGYRMVFSISGT
                     LGAIILATVLGWFITDARLLFIILGVGLGLASIIPPLVVFNITHEKPADEQPEPLSWW
                     QSMQLTLSNQPFRMVMGLYLLSWTTASILAAILIYFANYRLRVPEQANYFVLLAEGAA
                     IFFIPFWVWVAKKLDKRRAFIIGTLTWIFVLLGISALPADAIGLAYVLAALSGSGIAA
                     AYVLPWSMIPDIIEHDQLQTGQRREGSYYAFASFFQKLATAFALWAMGQALAANGYIT
                     PNSSGPLPTQPDSAIQAIRFFAGPAPAVLLILAVLFAWNYPITRESHSETLRVLAERE
                     S"
     gene            3327..3596
                     /locus_tag="PKI33_03225"
     CDS             3327..3596
                     /locus_tag="PKI33_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36043.1"
                     /translation="MKEYQVATEYIVSPEENINFPENVLTAIGKLRKVKEISSSTDIF
                     QCPECDTYYFYRASYEYLVSGSGSYDEYVLTREDDEIVKKYLQDD"
     gene            3608..3895
                     /locus_tag="PKI33_03230"
     CDS             3608..3895
                     /locus_tag="PKI33_03230"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNM36044.1"
                     /translation="MIIAFKGKIFQWRGPAPYLFVAVPEKESANIKAVSKLVTYGWGV
                     IPVVAKTGKTEWKTSLFPKDGRYLVPIKMVVQKAEKLKEGDEIKLQLELNL"
     gene            3985..>4701
                     /locus_tag="PKI33_03235"
     CDS             3985..>4701
                     /locus_tag="PKI33_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNM36045.1"
                     /translation="MHNPEIIANEKEEFKQAVLNARPFKPLYVKFKVFYGCNLKCEMC
                     NHWRETREPPVTPERLKEVISELAEMGTKKIHISGGEPMLRPQIPDFVELASSLGIKV
                     TMTTNGTLITKEKAKRLVEAGLRGVNISIDSPIRKMHEKIRGVEGAFKLTTKAVALFN
                     RYKHKGKLTIRINTVVSRTNYETLATLPDLAHELGADGINLIPVDDHCGEILSMRKKD
                     IAKFNEEIAPRIAERALELGL"
BASE COUNT         1258 a         1113 c         1100 g         1230 t
ORIGIN      
        1 gccaagaata atttataagc ataaagattt tgtggggttt ctcaccttga cggaatagaa
       61 aatacgatct attatcaaca aaggagaaat ttcatgaaaa aagaaatcac catcacgtca
      121 attcgggttg cagacttgct tgcagaaggt gaacggatgc ccgtctatgt acatgtcatt
      181 gaccatccaa atggacgtgt gttagtggat acagggttca ccgagttgca cccagcgata
      241 gctgaccttg acccgcgcat ctttcccttg aacgagcaga atgttgacct tgccagcatt
      301 gatatcgtcg tcaatacaca tttgcacttc gatcattgcg gtggcaatca cctcttcccc
      361 ggtaaaccga tctacgttca gcaagcagag ttggatgatg ctctcaccaa agacgactac
      421 accattcgcg agtgggttga tgcgcccggg gtgaaatata tgccggtcga tggtgagttt
      481 gaactgcttc ccgggttgag gcttgtccct gcaccgggtc atacccgtgg gatgcaaatg
      541 gtcgtcgttg agacaggtga gcgtccgatc atcatcgtcg gcggtgacgt ggcagtttgg
      601 ttcggcgaac ttgatgagcc gaaaactgaa ggtcaactaa aagtgcatac acttaacccg
      661 cagttggttt ggctcacaca cgagcatgag ccgtggcgac cgaagtaatt aatcaaactt
      721 gatatattca aagaactcta tcaagaggta aaatagccgt cattaatatg gcgtattcca
      781 tcaaatatcc ccgcaaggta tttattcgaa gtacgttccg ttttaccatt cgctcgcttt
      841 tccccttatt agcacaaaca aaaattacag ggtcaaaaaa cttcccacaa aacggacctc
      901 ttattctggt tggaaatcac actggcgcaa tggaagtcgt tctcatgggg ttgtattctc
      961 ccaagcccgt agagttcatg ggtgcaatgg aaatgccctg gaatggttgg atgggaaaaa
     1021 tgatcgacct atatggattg atccctgtcc accgtgggta taccagcaac acaactatga
     1081 aaatgggaat tgatgttttg cgtcagggtg gaatattggg aatttttccc gagggcggtt
     1141 tttgggagcc cggtaagcaa aaggcacaat caggggtagc atggttaagt tatgcggctc
     1201 aagcgccaat tctccccatt ggctttggcg atacgcgcgg caaaatggca gaaatatttc
     1261 aactaaaacg ccctgccttt gagatgaata ttggagatgt aattccgcct gtgcaattgg
     1321 aaaaatctgc acacaaaaaa gaggaattgc aacaagccgc agaccgtatt atggatgcag
     1381 tttgggcgtt ggtaccagaa gacgagcgca agcgcaaaga aagtcaacct gaaaacgaat
     1441 tatttacttt tgatatatcc atctttgacc aacatggaac gcccataccg attccagcag
     1501 atttacagct aacagacggc tcatggatca gtcgcttcgc acaccgcccg aacttgatcg
     1561 attcgatccg agactatatc ttcatcccgg tacaggttct aaaagaacta caccgaaagc
     1621 ctcctgcaga agaaatttac ttggcagcac attctatgct tgaatatgta gagcgcgata
     1681 accctcaata ctttaattat cgctatgggt ataaagacgg cgaagtcttt cagcaaagtt
     1741 ttcgtcaact acgcgacctg atgaaatggg caatggagaa tcaatatcag gtggcagcag
     1801 aagcccggta tgagtataca gacccaagta cgggcgagag gcgggtttta cacgtcccac
     1861 aagaattgga acaatggtag atcaaaaata ctttgcccgc catctaaagg agcagacatt
     1921 ccatgagcaa ttctgagcgt ttatccactc gtttcaaaat gttattcagt accggggatc
     1981 taagcacaag tattccgctt gcgatcttga tgttctttca actttatttt ctaaccgatg
     2041 ttgcccgcct gcgaccggac tatgccgggt gggcaattgc tctgccgcgt ttatgggacg
     2101 ccatcaacga cccacttttt gggttgctct cagatcggtt gcgcactcgt tggggacgcc
     2161 gccgtgcctt attacttttt gggtctgtgc cactcggttt aagttttatt ttgatgtgga
     2221 tcgtaccgcc ctttggtcaa cttggattgg cgatttatta tgcgttgacc tttattgttt
     2281 ttgatacggt atttaccgtc gtccatgttg gctacaactc actcacaccc gagctaactt
     2341 ctgattacga cgagcgtagt tcattgaacg gctatcgcat ggttttctcg atcagtggca
     2401 cattgggtgc tattattctt gcaaccgttt taggttggtt cattaccgat gcacgtttat
     2461 tattcatcat tctcggcgta gggctgggtt tggcttcgat cattccgcct ttggttgttt
     2521 tcaacatcac tcacgaaaag ccagccgatg aacagcctga gccgctttca tggtggcagt
     2581 cgatgcaatt gaccttgagc aatcaaccgt ttcgaatggt tatggggtta tatcttctct
     2641 catggacaac cgccagcatt ctcgccgcca tcttgattta ctttgcaaac tatcgtttac
     2701 gtgtgccaga gcaagcaaat tattttgtat tgttggctga aggtgccgca atctttttca
     2761 ttccgttctg ggtgtgggtt gccaaaaagt tggataagcg ccgtgccttc atcattggca
     2821 cattgacttg gatattcgtc ctgcttggca tctcagctct gcctgccgat gccattgggc
     2881 ttgcatatgt gctcgcggct ctttccggat ctggcattgc cgctgcatat gttttgcctt
     2941 ggtcaatgat cccagacatt atcgagcacg accaacttca aacaggtcaa cgccgcgagg
     3001 ggtcttacta tgccttcgct tctttcttcc aaaaactcgc cacagctttt gccttatggg
     3061 caatgggaca agccctcgct gcaaacggat acattacccc aaattcaagc ggacctctgc
     3121 caacccaacc tgattctgcc attcaagcca tccgcttctt tgccggaccc gctcccgccg
     3181 tcttgctcat tttggcagtt ctctttgcct ggaactatcc catcacccgc gaaagccaca
     3241 gcgaaacatt aagagtcctc gccgaacgcg aatcataggg aggcagtgtg accgaaaagc
     3301 atacaagctg tagcatttgt agcaatttga aagagtatca agttgctaca gagtacatcg
     3361 ttagcccaga agagaacata aattttcctg agaacgtgtt gactgctatt ggaaagttaa
     3421 gaaaagtaaa agaaatctca tccagcacag atattttcca atgccctgaa tgtgacacct
     3481 attattttta ccgtgcaagt tatgaatacc tagtcagcgg cagcggctcg tatgatgaat
     3541 atgttttgac ccgcgaagac gatgagattg taaaaaagta tttgcaagac gattaggaga
     3601 caccagtatg atcatcgcat tcaaaggcaa gatatttcaa tggcgtggac cagcccctta
     3661 tcttttcgtg gctgtcccag aaaaagaaag tgccaatatc aaagcggtct ctaaactggt
     3721 gacgtatggt tggggggtga ttcccgttgt agcgaaaaca gggaagacgg aatggaaaac
     3781 ttccctcttc ccgaaagacg gtcgctatct tgtgcctatt aagatggtag ttcaaaaagc
     3841 ggaaaagctc aaagaaggcg atgaaatcaa actgcagctt gaattaaatc tttaaatatt
     3901 atgaacgaag cctttgcctg ataaaccgag caagggcttt atttttccat tagtacgagt
     3961 gatacaatta tcaaacttat tgttatgcat aaccctgaaa taatcgccaa cgagaaagaa
     4021 gaattcaaac aagcggtgtt gaacgcgcgt ccgttcaagc cgctttatgt taagttcaag
     4081 gtcttttacg gctgcaacct caaatgtgag atgtgcaatc actggcgcga aacccgcgag
     4141 ccgcctgtta cgcctgaacg cttgaaagag gtcatctctg agttggcaga aatgggcacg
     4201 aagaaaattc acatctctgg cggcgaaccc atgctgcgac cgcagattcc cgattttgtg
     4261 gagttggctt cttcgcttgg catcaaagtg accatgacca ccaatggcac actcatcacc
     4321 aaagagaaag ccaagcgact ggtggaagca ggtttacgcg gcgtgaatat ctccatcgac
     4381 tcgccgatac gcaagatgca tgaaaaaatt cgcggcgtgg aaggcgcgtt caagttaacc
     4441 accaaagccg ttgcgctttt taaccgttac aaacacaaag gcaagctgac catccgtatc
     4501 aacaccgtgg tcagccgcac aaattacgag acgcttgcca ccctcccaga ccttgctcat
     4561 gaactcggcg cggacggcat caatctgatt cctgtggatg atcactgcgg cgagatcctg
     4621 tccatgcgca agaaggatat cgccaaattc aatgaagaga tcgcaccgcg cattgccgaa
     4681 cgcgcgcttg aattagggct g
//
LOCUS       DAOREF010000106         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10489_length_4687_cov_5.526770, whole genome shotgun sequence.
ACCESSION   DAOREF010000106 DAOREF010000000
VERSION     DAOREF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10489_length_4687_cov_5.526770"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..793)
                     /locus_tag="PKI33_03240"
     CDS             complement(98..793)
                     /locus_tag="PKI33_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNM36046.1"
                     /translation="MTDIKTILTHGNYELRGQFMLGSNYTFLVDVHHEDEVVKAVYKP
                     SKGEQPLWDFPDNTLALREVAAYELSETLGLHIVPVTVYRDDGPHGPGSLQQYIEYDI
                     EYHYFNFTVEDKQKLRPVVFFDILANNADRKGSHVFFEDETHRLYAIDHGICFHEENK
                     LRTVLWDFAGQAIPDELLAPLSLTGNWSALFEPYLSPREIDALLFRAEELCESKVFPR
                     PLQGRRAYPYPPI"
     gene            complement(834..1361)
                     /locus_tag="PKI33_03245"
     CDS             complement(834..1361)
                     /locus_tag="PKI33_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HNM36047.1"
                     /translation="MSRTDIDVDPCDHITADAIGKPGQRVFYLHAYSEHRTYTIIIEK
                     AQLLSLAIGVEQFLGQLSQQYPDLEEASGDYVEEVMRINPPVDPLFRAGEIGLGYDKD
                     RDRVVVFAKELITEEQDPEEASQIRFWATRTQLRQLARWGQEVSQRGRPICPQCGQPM
                     EAGGHFCPKKNGHLH"
     gene            1517..3196
                     /locus_tag="PKI33_03250"
     CDS             1517..3196
                     /locus_tag="PKI33_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNM36048.1"
                     /translation="MYDKKKLDEIQQSLSKWEETSLKKALNQLPERADEFITTSSEPI
                     NRLYTPLDIADLDYNDSLGNPGEYPYTRGVHPTLHRSKLWTMRMFAGFGTAEETNGRF
                     KYLLEQGQTGLSIAFDLATLMGYDTDQPEALGEFGKCGVAVSSLKDMEILLDGIPLDK
                     VSSSMTINSPASIIWAMYLVAAEKQGVRLDQLRGTLQNDILKEFIAQKEFIFPPEPSM
                     RLVVDTMEYGSQHVPQWNTISISGYHIREAGSTAAQELAFTLADGMEYVRWGIARGMD
                     VDEFAPRLSFFFNAHNDFFEEIAKYRAARRIWAREMRETFKAKNPRSWLMRFHTQTAG
                     VSLTAQQPENNIVRVAIQALAAVLGGTQSLHTNSMDEALALPSEHAVKVALRTQQIIA
                     EESGVTNTVDPLGGSFFVEAQTDRMEKDAYAYFQRIQDLGGVIPALEKGFMQSEISDA
                     AYRYQREIDENRRKIVGVNAYLDEKPAAIPILKMDPNGYSRQMNRLNEVRKTRDSGRV
                     GQTLDALRIACEGTENTMPYILDCVRAYCTLGEIIGVMTKVFGKYEEPTMI"
     gene            complement(3232..>4687)
                     /locus_tag="PKI33_03255"
     CDS             complement(3232..>4687)
                     /locus_tag="PKI33_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36049.1"
                     /translation="TLARNFEGAMSRKLPFWLKTLYGSGDWGIASISMMRSIFYAIYL
                     TDVVGIEPRLASLGALVGIVWDAVNDPIIGMLSDRVKSKLGRRRPFLLWFAFPFGLSF
                     VMLWSAPNWDSQVALLIYVTLAFMISDTLTTLVAVPYYSLTPELTQNYDERTSLSSFR
                     SVFQLLSAMVVVVSAPMIVDAVISGGGTQQQGFLTAGAVFGAIGSLPLFLIGLFIRER
                     FAEDTQEQEELSFRETLKLAWQNVPFRYAVGIYMFNWSAVDMVSITFPFFLLYWVAQG
                     DLLAKINILGVDLALESAFFGVLMLVCVLFVPFWLWLSKRQNKIRAYILGMAAWVVVQ
                     VLIFTIQPGETSYMLFLAGLAGIGVSAAYILPDSILPDVIEWDELRTGRRQEGIYYGI
                     RTLIRKLTGALIIFITLQILGWSGYQAPPENVTVYQQPESALFMIRLMDSFIGAAILA
                     GTIILAWSYPLTREKYQKIQKLLAVRRNKNAETT"
BASE COUNT         1282 a         1185 c         1152 g         1068 t
ORIGIN      
        1 tccgtcattg cgaggaaggt gttcttccca acgaagcaat cttcaacacg ttattagatt
       61 gcttcgggca aaaaacaaga gcgccctcgc aatgacgtta aatgggcggg taaggatacg
      121 cccttcgtcc ttgtaacggg cgtggaaaga cttttgactc gcacaattct tctgcgcgaa
      181 agagtagggc atcgatctcg cgcgggctga gataaggctc aaagagggca gaccagtttc
      241 cggtcaacga gagaggcgcg agcaattcgt caggaatggc ttgaccggca aaatcccata
      301 atacggtgcg gagtttattc tcctcatgaa agcaaatgcc atggtcgatg gcatagaggc
      361 gatgggtttc atcctcaaag aagacatggc ttcctttgcg gtcagcattg ttggcgagaa
      421 tatcgaagaa gacaacggga cgaagttttt gcttatcttc caccgtgaag ttgaagtagt
      481 gatattcgat atcgtattcg atgtattgct gtaaagagcc gggtccatgc ggaccatcgt
      541 cgcgatagac ggtgactggt acgatgtgta agccaagcgt ttcgcttagt tcatatgcgg
      601 cgacttcgcg caacgcaagg gtgttgtcgg gaaaatccca aagcggttgt tcgcctttgc
      661 tgggtttgta tacggcttta acaacttcat cctcgtggtg aacatctaca aggaaggtgt
      721 aattcgagcc caacatgaac tgaccacgaa gctcgtagtt gccatgagtg aggatggttt
      781 tgatatcggt catgggaaac gtttaagcgt tcgaacgttt tcacgtttaa acgttagtga
      841 aggtgtccgt ttttcttggg gcagaagtga ccgcccgcct ccatgggttg accgcattgc
      901 gggcaaatgg ggcgaccccg ttgagagact tcctgccccc aacgggcgag ctgacgcagt
      961 tgggtacggg tcgcccaaaa acggatctgc gacgcttctt cggggtcttg ttcttcagtg
     1021 atgagttctt ttgcgaagac gactacacgg tcgcggtctt tatcgtaacc caagccgatc
     1081 tcaccagcgc ggaagagcgg gtctacgggc gggttgatgc gcatgacttc ttcaacataa
     1141 tcgccagaag cttcttcaag gtcggggtat tgctggctga gctgaccaag gaattgctcc
     1201 acgccaatgg caagtgaaag caactgcgct ttttcaatga tgatagtgta ggtgcgatgc
     1261 tcggaataag catgcagata aaaaacgcgt tgaccaggtt tgccaatcgc gtcagcagtg
     1321 atgtgatcac aggggtcgac gtcaatatca gtgcgagaca taaagattac cttttttgag
     1381 agtataccag aatagccatc cccacccatc ctccctaaaa tgtgaggaaa gttaattcat
     1441 tacatatggg aagagatgcc cgtgatagta ggaggagggg gcggtataat ctcatcaact
     1501 cgccaaagga gatgccatgt acgataaaaa aaagctcgat gaaatacaac aatcgctttc
     1561 caagtgggaa gagacttctc tcaaaaaagc tttaaatcaa ctccctgaac gtgctgacga
     1621 atttattact acttcctccg aaccgatcaa cagactttat acaccgctcg atattgccga
     1681 tttagattac aacgactcgc tcggcaaccc aggtgaatat ccatacacac gcggagttca
     1741 cccaacgttg catcgctcca aactgtggac catgcgtatg tttgcaggct ttggtactgc
     1801 agaagagacg aacgggcgct tcaaatactt acttgagcag ggtcagaccg gcttgagcat
     1861 tgccttcgac cttgccacgc tgatgggcta cgatactgac caacccgaag cgctgggcga
     1921 atttggcaaa tgtggtgtag ccgtttcttc gcttaaagat atggaaattc ttttggatgg
     1981 cattccgctc gataaagttt cttcgagcat gactattaac tcgccggctt ccatcatctg
     2041 ggcaatgtat cttgtagccg ctgaaaaaca aggtgtgcgt ctcgatcaat tacgcggcac
     2101 ccttcagaac gacattctca aagaatttat cgcacagaag gaattcatct tcccaccaga
     2161 accttccatg cgtctcgtag tggatacgat ggaatacggc tcacaacacg tgccgcagtg
     2221 gaataccatt tccatttcgg gctatcacat ccgtgaagcg ggttcgacgg ctgcgcaaga
     2281 gctggcgttc acccttgcag acggcatgga atatgtccgc tggggcattg cacgcggcat
     2341 ggatgtagat gaatttgcgc cgcgtctctc tttcttcttc aatgctcata acgatttctt
     2401 cgaagagatt gccaagtatc gtgcggctcg ccgtatttgg gcgcgagaaa tgcgcgagac
     2461 cttcaaagcg aagaatccgc gctcgtggct gatgcgcttc cacactcaaa cagcgggtgt
     2521 ctcgctcacg gcacaacagc cggaaaataa catcgtccgc gtggcgattc aggcgctggc
     2581 tgcggtattg ggtggaacgc aaagtttgca caccaactcg atggatgaag cgcttgcatt
     2641 gccctctgaa catgctgtga aagttgctct gcggacacag cagatcatcg cagaagaatc
     2701 cggcgtgacg aatacggtcg acccgcttgg gggtagtttc ttcgtcgaag cgcaaacaga
     2761 ccgaatggag aaagatgcgt acgcctactt ccagcgcatt caagatctag gtggagtcat
     2821 ccccgcgtta gaaaaaggtt tcatgcaaag cgaaatctcc gatgcggcgt atcgctatca
     2881 acgtgagatc gatgagaacc gccgcaagat cgtgggcgtc aatgcgtatc tcgatgaaaa
     2941 acctgccgcc attccaatcc tcaaaatgga cccgaacgga tacagccgtc agatgaatcg
     3001 cctgaacgaa gtccgcaaga cgcgcgatag tgggcgcgta gggcaaactc tcgacgcttt
     3061 gcgcatcgct tgcgaaggga ccgagaacac catgccgtat attttggatt gtgtgcgtgc
     3121 ctattgcaca ttaggggaaa tcatcggtgt gatgacgaag gtttttggaa agtacgaaga
     3181 accaacgatg atctaggcgc tctcgccttt tatagcaaaa cgcggaaacg atcaagtcgt
     3241 ttccgcgttt ttgtttcggc gaacagccag taacttttga atcttctgat atttttcacg
     3301 tgtgagcgga taagaccatg ccagaatgat cgtgcccgcc aaaattgctg cgccgatgaa
     3361 cgagtccatt aggcgaatca tgaacaaggc agattccggt tgttggtaca cggtcacatt
     3421 ttctggcggc gcctgatagc cagaccaacc caaaatttgc agcgtgatga aaatgatcaa
     3481 cgccccggtc aacttacgga tgagcgtacg gatcccataa taaatacctt cttgtctgcg
     3541 ccccgtacga agttcatccc attcaatcac atctggcaaa atcgagtctg gcaaaatgta
     3601 agcggcagaa acgccaatac cagccaaccc ggcaaggaac aacatgtaac tggtttcgcc
     3661 cggttgaata gtgaagatca acacctgcac aacaacccac gccgccatac caaggatgta
     3721 cgctctgatc ttattttgtc gtttcgatag ccacagccag aacggcacga acaacacaca
     3781 aaccaacatc aacaccccaa agaaggcaga ctctaaagca aggtctacac caaggatatt
     3841 gatctttgcc aataaatctc cctgtgccac ccaatacaaa aggaagaacg gaaatgtgat
     3901 cgagaccatg tccacggcag accaattgaa catgtaaatt cccactgcat accgaaatgg
     3961 cacgttctgc caagccagtt tcagggtctc acgaaatgaa agttcctctt gttcctgtgt
     4021 gtcttccgca aatcgttcac gtataaaaag accaatcaag aacaatggca acgaaccaat
     4081 cgcgccgaac accgccccag ccgtgagaaa accttgttgt tgcgtgccgc cgcctgaaat
     4141 cacggcatcc acgatcatgg gcgcagaaac caccactacc atcgcagaga gcaattgaaa
     4201 gaccgagcga aaacttgaaa gcgatgtacg ctcatcataa ttttgagtca actcgggggt
     4261 gagcgaataa tatggaaccg ccaccaacgt tgtcagcgtg tctgagatca tgaacgccaa
     4321 cgtcacatag atcaacaaag caacctgact atcccaattc ggtgcagacc ataacatcac
     4381 aaaactcaac ccgaatggga aggcgaacca cagcaaaaac ggcctacgcc tgccaagttt
     4441 tgatttcacc ctgtcactca gcatcccaat aatcgggtca ttgacggcat cccacacaat
     4501 tccaaccaat gcacccaacg atgccagccg tggctcaatg ccaaccacat ctgtcaagta
     4561 aatggcataa aagatcgagc gcatcatact aatgctggca attccccaat cacccgaacc
     4621 atataaagtc ttcaaccaaa atgggagttt tctgctcatc gcaccttcaa aatttcgcgc
     4681 aagtgta
//
LOCUS       DAOREF010000107         4682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10506_length_4682_cov_4.774368, whole genome shotgun sequence.
ACCESSION   DAOREF010000107 DAOREF010000000
VERSION     DAOREF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10506_length_4682_cov_4.774368"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="PKI33_03260"
     CDS             complement(<1..2260)
                     /locus_tag="PKI33_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNM36050.1"
                     /translation="MTKSFTLIKEQQIPEINSLVQLWEHKRTGARLLSVINDDENKVF
                     SINFRTTPKDSTGVAHILEHSVLNGSEKYPVKEPFVELLKGSLATFVNAFTFPDKTCY
                     PVASQNEKDFYNLIDVYIDAVFNPLLKEETLMQEGWHYEIEDPSAPLTYKGVVFNEMK
                     GAYSSPDNYLAKVIIESLFPKHIYGVDSGGNPAEITNLTYENFFAFWETYYHPSNSFI
                     FFYGNDDPDTRLKLMDDYLKPFKKKKVKSAVPLAKPFKKAKKLEFPYLAIEGEDLDKK
                     HFLTVNWKLPDASDPVQAMSFRILTHALIGTPASPLYKALTDSGLGEDLAGLGLESDL
                     REMIFSTGLKGTKARSAKKVETLIFDTLQKLVNEGIDPDTIAASINTLEFAMRENNTG
                     GFPRGIAIMLRALSTWLYDDDAFKTLAFEAPLAEIKNRLAADSRYFEKMIQTHLLDNV
                     HRTVIRLTPDVELAQRLEAEETLRLDSARKTMSDDDIRKHIENTEKLKIRQETPDNPE
                     DVAKLPVLDLEDLDKQARTIPIEEIQVQDSKVLYHDIFTNGIVYLDLAFDMRAMPQEL
                     LPLTEVFARALFEVGTETEDYVKLSQRIGKSTGGIYGSSVTATTQGSRESRAYFVIRG
                     KSTMSQANEMLSILRDVLLTVKFDNKERLKQIVMEEKAGLESGLAPAGHRFANMRLRS
                     QFGGTGYINDQTKGIGYLFALRELATEIDKKWANVLKKLETIRETLINSKTLLCNVTL
                     DAANWKTFQPHLE"
     gene            complement(2309..3466)
                     /locus_tag="PKI33_03265"
     CDS             complement(2309..3466)
                     /locus_tag="PKI33_03265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNM36051.1"
                     /translation="MSANCSRLKGMCNGVGDGYNLGVPTLPLNCILQGNCIDVLNSLP
                     EKSVDLIFADPPYNLQLQNDLFRPNLSKVDAVDDNWDKFSSFSKYDEFTSDWLRAARR
                     VLKDTGTIWVIGSYHNIYRVGAVMQDLGYWILNDVVWLKTNPMPNFRGVRFTNAHETL
                     LWAQKEQGAKYTFNHKAMKALNDDLQMRSDWIIPLATGKERLKTNGTKTHPTQKPEAL
                     LYRVILSTTNPGDVVLDPFFGSGTTGAVAKKLGRNFIGIERETKYIKAAQKRIDAIKS
                     APAEALDLPEKRNQERIPFGALVENGYLKPGQKLQYAKGKREATILANGHIKCGKLIG
                     SIHMVARELTNAPANGWEMWLYSERGTLKPINELREKFRKLQVKSNKKIRD"
     gene            3490..>4682
                     /locus_tag="PKI33_03270"
     CDS             3490..>4682
                     /locus_tag="PKI33_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM36052.1"
                     /translation="MALISFQEVRIGFGGHPLLDDVSFQVERGERVGLLGRNGMGKST
                     LLKMLNGDVEPHDGVIARQQNVRAAYLPQEVPQDLHGRIADIIASGLIDTDFADDEHH
                     WKRDNQVQKTISLMELDGDARFETLSAGMKRRVILACGLVCNPDLLLLDEPTNHLDIQ
                     AIDWLEDFLKRWGGTLVFVTHDRVFLQKLTTRIIELDRGSVYDWACDYPTFLERKEAM
                     LAAEQTQNALFDKKLAQEEAWIRKGIEARRTRNEGRVLALKKLRQQRSERRELLGKVK
                     MQIGTEKRSGRLVIETENISYAYGEKTIIHNFTTTIQRGDKIGIVGANGSGKTTLLKL
                     LMAQLQPQTGEVHHGTNLDIVYFDQLRTQLDESKSVLDNVGQGRDTVTINGRTRNLMG
                     YLEDFL"
BASE COUNT          973 a         1168 c         1344 g         1197 t
ORIGIN      
        1 tctcgagatg tggttggaag gttttccagt ttgcggcgtc gagcgtcaca ttgcaaagca
       61 gagtcttgct gttgatcaac gtctcgcgga tggtctccag cttcttcagc acattcgccc
      121 attttttatc gatctcggtg gcaagttcgc gcaacgcaaa cagatagcca atacctttgg
      181 tctggtcatt gatatagccc gtgccgccaa actgcgagcg caggcgcatg ttcgcaaagc
      241 gatgtcccgc aggtgccaag ccagactcca agcccgcttt ctcttccatc acaatttgct
      301 tgaggcgttc cttgttgtcg aacttcacgg tcaggaggac atcccgcagg atgctcaaca
      361 tctcgttggc ttgactcatg gtggacttgc cgcggatgac gaaatacgcg cggctctctc
      421 ttgacccttg cgtggtcgct gtgaccgacg agccgtagat tccgccggtg cttttcccga
      481 tccgctgcga cagtttgacg tagtcttcgg tttcggtgcc cacttcgaac aatgcgcgtg
      541 cgaagacttc cgtcagcggc aggagttcct gcggcatggc gcgcatgtcg aaggcgaggt
      601 cgagatagac gatgccgttg gtgaagatgt cgtgatacaa gacctttgag tcttgcactt
      661 gaatttcttc aattgggatc gtgcgggctt gcttgtctaa atcttctaaa tcaagcacag
      721 gaagtttggc aacatcttcg gggttgtcgg gagtctcctg acggatcttc aacttctcgg
      781 tgttctcaat atgcttgcgg atatcatcat cgctcatggt ctttctggca gaatccagcc
      841 tgagcgtttc ctctgcttcg aggcgctgag ccagttccac gtcaggagtg aggcgaatga
      901 ccgtgcggtg gacgttatcc agcaggtgag tctgaatcat cttttcgaaa tagcgcgagt
      961 cggcggcgag tcgattcttg atctcggcga gtggggcttc gaaggcgagg gttttgaagg
     1021 cgtcgtcatc gtagagccag gtggagaggg cgcgcagcat gatggcgatg ccgcgtggga
     1081 agccgccggt gttgttttcg cgcatggcga attcgagcgt gttgatcgag gcggcgatgg
     1141 tgtcggggtc gatgccttcg ttgacgagtt tttgcagagt gtcgaagatg agtgtttcga
     1201 cctttttggc gctgcgggct ttggtgcctt tgagaccggt ggagaagatc atctcgcgca
     1261 agtcgctttc gagaccgaga cctgcgaggt cttcgcccaa gccggagtcg gtgagcgctt
     1321 tataaagtgg ggaagcgggt gtgccgatga gggcgtgtgt gaggatgcgg aaactcatcg
     1381 cctgcacagg gtcggaggcg tcgggcagtt tccagttgac ggtgaggaaa tgttttttgt
     1441 cgaggtcttc gccttcgatg gcgaggtagg ggaactcgag tttcttggct ttcttgaacg
     1501 gcttggcaag cggcaccgcc gatttgacct tcttcttttt gaacggcttg aggtagtcat
     1561 ccatcaattt gaggcgcgtg tcggggtcgt cgttgccgta gaagaaaata aacgagttgg
     1621 aggggtggta gtaggtttcc caaaatgcga agaagttttc gtaggtgagg ttcgtgatct
     1681 ctgccgggtt gccgccagag tccacgccgt agatgtgttt ggggaacagc gactcgatga
     1741 tgaccttggc gaggtaatta tcgggcgaag aatacgcgcc cttcatctcg ttgaagacca
     1801 cgcctttgta cgttaacggc gcggacgggt cttcgatctc gtagtgccag ccttcctgca
     1861 tgagcgtctc ttctttgagc aggggattga agaccgcgtc aatgtacacg tcgataaggt
     1921 tgtagaaatc tttttcgttc tggctggcaa ccgggtaaca agttttatcg gggaaagtga
     1981 aggcgttgac gaaggtcgcc agtgagcctt tgagcaattc cacaaacggt tccttgaccg
     2041 ggtatttctc cgagccgttt agcacggaat gttcgaggat gtgcgccaca ccggttgagt
     2101 ctttaggcgt ggtgcgaaag ttgatgctga atactttgtt ttcgtcgtca ttgatgaccg
     2161 agagcaggcg cgcgccagta cgtttgtgtt cccacaactg cacgagtgag ttgatctcgg
     2221 ggatttgttg ttctttgata agggtgaatg atttggtcat gggttccttt gtggtaactg
     2281 gtaattggag attagtaatt accaatctct aatctcttat ttttttgtta ctcttaactt
     2341 gtaactttct aaatttttct cttagctcat taattggctt aagtgtaccg cgttctgaat
     2401 aaagccacat ttcccagccg ttggcggggg cgttcgtcag ttcgcgggca accatgtgga
     2461 tcgagccgat gagtttgccg catttgatat gtccattggc gaggatggtc gcttcgcgtt
     2521 tgcctttggc gtattggagt ttttgccccg gcttgagata accattctcc accaacgctc
     2581 caaacgggat ccgttcctgg ttgcgcttct cgggcaggtc caacgcctca gccggcgcgg
     2641 acttgatcgc gtcgattcgt ttttgcgcgg ctttgatgta cttcgtctca cgctcaatgc
     2701 cgatgaagtt acgtccaagt ttttttgcga cggctccggt cgtgccgctg ccaaagaagg
     2761 gatcgagcac cacgtcaccg gggttggttg tggataggat gactcggtat aagagggctt
     2821 ccggcttttg ggtgggatga gtcttcgtcc cgttggtttt gaggcgttct tttcccgtcg
     2881 ccaaagggat gatccaatca ctgcgcattt gcaggtcatc gtttagggct ttcatcgcct
     2941 tgtggttgaa ggtgtatttc gcaccttgtt ccttttgcgc ccagagcagg gtctcatgcg
     3001 cgttggtgaa gcgcacgccg cggaagttcg gcatgggatt ggtcttgagc caaaccacat
     3061 cgttgagaat ccaatagccc agatcttgca tgaccgcgcc aacgcgataa atgttgtgat
     3121 atgagccgat gacccagatg gtgcctgtat ctttcaaaac ccgccttgct gcgcgtagcc
     3181 aatcagaggt gaattcgtcg tatttggaga aactggaaaa cttgtcccag ttatcatcca
     3241 ctgcatccac tttgctgagg ttagggcgga agagatcgtt ctgcaattga agattgtatg
     3301 gagggtcggc aaagatcaag tccaccgatt tttccggcag tgaattgagc acgtctatgc
     3361 agttgccttg cagaatgcag ttgaggggca gggtgggcac gcccaaatta tacccgtcac
     3421 cgactccatt acacatcccc tttaatcggc tacaattcgc gctcatgaaa tcatcccaga
     3481 ggtttttgca tggcattaat tagttttcag gaagtccgca ttggctttgg cgggcatcct
     3541 ttattggacg atgtcagttt tcaagttgag cgcggcgagc gcgttgggtt gttggggcgt
     3601 aatggcatgg gcaagtctac gctgttgaaa atgctcaatg gtgatgtcga gccgcacgac
     3661 ggcgtgattg cccgccagca gaatgtccgc gcggcgtatc tcccgcagga ggttccgcaa
     3721 gacctgcacg gacgcatcgc ggacatcatc gcttcgggtc tcatcgacac cgactttgcc
     3781 gacgacgaac atcattggaa gcgcgacaat caagtgcaga aaaccatctc attgatggaa
     3841 ctcgacggcg acgcgcgttt cgaaactctc tccgccggga tgaagcgccg cgtcattctt
     3901 gcctgcggct tggtctgcaa cccagacctt ttgctgctcg acgaaccgac taaccatctc
     3961 gacatccaag ccattgactg gctcgaagac ttcctcaaac gctggggtgg gacgctggtc
     4021 ttcgtgacac atgaccgcgt ctttttacag aaactcacca cccgcatcat cgaactcgac
     4081 cgtggcagtg tctacgattg ggcgtgtgat tacccgacct tcctcgaacg caaagaagcc
     4141 atgctcgccg ctgagcaaac tcaaaacgcg ctcttcgata aaaaactggc gcaagaagaa
     4201 gcctggatcc gcaaaggcat tgaagcccgc cgtacccgca acgaagggcg tgtgctcgcg
     4261 ttgaaaaaat tacgccaaca gcgttccgag cggcgtgaac tgcttggcaa agtgaaaatg
     4321 cagatcggca ccgagaagcg ttcgggtagg ttggtgatcg aaaccgagaa catcagttac
     4381 gcctatggcg agaagaccat catccacaac ttcaccacca ccattcaacg cggagataag
     4441 atcggtatcg tcggcgcgaa cggttcaggc aaaacgacct tgctcaaact gctgatggcg
     4501 cagctccaac cgcagacagg tgaagtccat cacggcacga atctcgacat cgtctacttc
     4561 gaccaactcc gcacccaact cgatgaatcc aaatccgtgc tcgataacgt tgggcagggg
     4621 cgcgacaccg tcaccatcaa cgggcgcacg cgcaacctga tgggctacct cgaagatttt
     4681 ct
//
LOCUS       DAOREF010000108         4639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10656_length_4639_cov_4.857766, whole genome shotgun sequence.
ACCESSION   DAOREF010000108 DAOREF010000000
VERSION     DAOREF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10656_length_4639_cov_4.857766"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="PKI33_03275"
     CDS             <1..1520
                     /locus_tag="PKI33_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNM36053.1"
                     /translation="IRFMSVPFNLPPLLEKIVNVLPANTEIYLVGGAVRDLLTNRRSP
                     DFDFAVPSNGISLARTVANALKADFMVLDDERDTGRVIIIEDGTYTYLDFAAYRGATL
                     EEDLRDRDFTINAIALNLRDITVIDPLNGATDIRAKVIRACSPDSLSNDPVRILRAVR
                     QAAAFGFSIDKSTREWMKQAADKINNVSIERVRDEVFKMLGGPKASASIRALEMLGVL
                     QHLMPELLKMKGVTQSNPHIHEVWVHTLAVVDQLEDLLTSSLRLDFDPEKTSDMFMGL
                     ISLKIGRYREQIAKHFANQLNPSRSHRSLLMFSALYHDVCKPDTKTIDENGRIRFFDH
                     DIKGAEVTADRARAFNLSNDEVERLHTIVRHHMRFHFFADRLEREGQPPSRKAVYRFF
                     RDSGAAGVDLILLALADLRGTKGNELTQVTWTAYLDIARILLENYWEHPEEVVNPPRL
                     IDGNELMKELNLQPGKVVGTLLESIRENQAAGKIVDKEQALAFAREELAKGVNEA"
     gene            1517..2053
                     /locus_tag="PKI33_03280"
     CDS             1517..2053
                     /locus_tag="PKI33_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNM36054.1"
                     /translation="MNYVFFDLESQFLFDEVGGRDHADKLKVACAVTYSTAKNDFTVY
                     WENDVPALLAELKAATKVIGFNLISFDYRVLQPYANQVRLASIPTLDMLVDIHKNLGF
                     RVSLDNLASASLGATKTADGIQSVQWFRNGELDKVAEYCKADVDITRRVFEFGRDNGF
                     VYYKSKLGSKLKTPVKWK"
     gene            2060..2824
                     /locus_tag="PKI33_03285"
     CDS             2060..2824
                     /locus_tag="PKI33_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM36055.1"
                     /translation="MNKIKLNGIELAYERRGMGTPLMLIHGYPLDHSIWNDVAAQLEN
                     DFDLIIPDVRGFGESTTVDAPYSLSYIAYDLAALLDSLGVGPTALAGHSMGGYIALAF
                     AKSFPQRVTGLALVSSQAASDTPERKEGRYKTAADVVQKGVQIVADAMTDKLSPNQSV
                     RDFVRPLIAKQSVAGVTGALKAMAERDDMTSFIASYAQPLVLIHGDADELIPLERARE
                     IKSLVSSAQLVELAGVGHMPMLEMPNETASALKKLK"
     gene            complement(2888..3331)
                     /locus_tag="PKI33_03290"
     CDS             complement(2888..3331)
                     /locus_tag="PKI33_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36056.1"
                     /translation="MTPTAKILVIDDDAAITELMSMLLKTFGFEVLTTNIGEEGIRLA
                     REANPNVILLDLMMPDLDGWQVCKEIRKTSTVPILILSAINDPRMVASVLDVGADDFL
                     VKPVPSGVLVANIKKLIRRNTGALDMSKLKKTTASLQQANHALHP"
     gene            3535..>4639
                     /locus_tag="PKI33_03295"
     CDS             3535..>4639
                     /locus_tag="PKI33_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNM36057.1"
                     /translation="MSKWVYLYNEVKEVEKLAKGSWDGVKAIVGGKGAGLMDMTRANV
                     PVPPFFTVTTEACNAYQKAGKFPQGMWEQELKALKEIERKTGKKFGDPKNPLLVSCRS
                     GAKFSMPGMMNTILNIGLTDIVAEGMIKATNNDRFVWDSYRRVVEMFGTVVMDIEDEA
                     FEHPLEAYKEKKGYKLDIEMRAEDWKEVVGIYKQAYKKACGQEFPQDPYKQLSLATEA
                     VFRSWNGKRAVAYRRKEGISDALGTAVNICTMVFGNMGDDSATGVAFTRNPSTGEKKM
                     MGEYLLNAQGEDVVAGIRNTDAIENLKKYMPKAYAQFMEITARLEKHYTDMQDVEFTI
                     ERGKLWMLQTRVGKRTAKAAVKIAVDMANEGLIN"
BASE COUNT         1205 a         1039 c         1184 g         1211 t
ORIGIN      
        1 agattcgttt tatgtctgtt cccttcaacc ttcccccttt attggaaaaa atagttaatg
       61 tccttcctgc aaacacggaa atctatcttg tcggtggggc agtgcgggat ctattgacca
      121 accgccgctc tcccgacttc gattttgccg tgccttcgaa tggcatttct ctcgctcgaa
      181 ccgttgccaa cgcccttaaa gcggacttta tggttctcga tgacgaacgt gatacgggac
      241 gtgtcattat tatcgaagac ggtacatata cctatctcga tttcgctgcc tatcgtggag
      301 cgacactcga agaagacctt cgcgaccgcg actttaccat caatgcgatt gcgcttaatc
      361 tacgtgacat tactgtcata gacccattga atggtgcgac agatatccgc gccaaagtga
      421 ttcgtgcctg ctcacctgat tccttgtcca atgacccggt gcggattttg cgtgctgtgc
      481 gtcaggcggc agcatttggt ttcagtatcg acaaatccac tcgcgagtgg atgaaacaag
      541 cggcagacaa gatcaacaac gtttctattg agcgtgtacg agatgaagtc tttaaaatgc
      601 ttggcggacc caaagccagc gcgtccattc gggcgttaga gatgttgggc gttttacaac
      661 accttatgcc tgaattgttg aaaatgaagg gcgttaccca atccaatccg cacatccacg
      721 aagtgtgggt acacactctc gctgtggtag accagcttga agatttgctc acctcgtctc
      781 tacggttaga ctttgacccc gaaaagacca gcgatatgtt tatggggctg attagcttaa
      841 agatcgggcg ttatcgcgaa caaattgcta aacattttgc caatcaactc aacccaagcc
      901 gctcccatcg ttcgttgttg atgttttctg ctttgtatca tgacgtctgc aagccagaca
      961 ccaaaaccat cgacgaaaat gggcgcatcc gcttctttga ccacgacatc aaaggggcag
     1021 aagtgaccgc agaccgggcg cgggctttca acttaagcaa tgatgaagtc gagcggttgc
     1081 atactatcgt tcggcatcat atgcgcttcc atttctttgc cgatcgcttg gaaagagaag
     1141 ggcagccgcc ttctcgcaaa gcggtgtatc gcttctttcg cgatagcggc gccgctgggg
     1201 tcgatttaat cctcctcgcc ttggctgatt tgcgcggaac caaaggcaac gaactgaccc
     1261 aagtgacttg gacggcgtat ctcgatattg ctcgtatttt gctggagaat tattgggaac
     1321 accccgaaga agttgtcaac ccgccgcggt taatagatgg gaacgagttg atgaaagaac
     1381 taaatctcca gccaggaaag gttgttggga cgttgcttga gtcgattcgc gagaatcaag
     1441 ccgctggcaa gatcgtggat aaagaacaag ctttagcgtt tgcgcgtgaa gaacttgcga
     1501 aaggagtgaa tgaagcatga actatgtatt cttcgacctc gagtctcaat ttctttttga
     1561 tgaagtaggc gggcgtgatc atgccgataa attgaaggtc gcatgcgcgg tcacatattc
     1621 cactgcaaaa aatgacttta cagtgtattg ggagaatgat gtccctgctt tgctggcgga
     1681 gttgaaagcc gctaccaagg tcattgggtt caacctcatt agctttgatt atcgagtctt
     1741 gcagccgtat gcaaatcagg ttcggctcgc atccattcca actttggata tgttggtaga
     1801 tatccacaag aatttagggt ttagagtgag ccttgataat ttggcgagtg catctcttgg
     1861 ggcaaccaaa acagcagatg gcattcagtc tgtgcagtgg tttcgtaacg gcgagctgga
     1921 taaagttgct gaatactgca aagccgatgt agatatcact cggcgcgtct ttgaattcgg
     1981 gcgcgataat ggcttcgtat attacaagtc taagctgggc agcaagctga aaaccccggt
     2041 caagtggaag taaagcgata tgaacaaaat caaacttaac ggtattgaac tggcatacga
     2101 acggcgcggc atgggaaccc ctttgatgct catccacggc tatcctttgg accattcgat
     2161 ttggaatgat gtcgctgctc aactcgaaaa tgatttcgac ctgatcatcc ccgatgtgcg
     2221 cggattcggt gaatcaacta ctgttgacgc accttactct ctctcataca ttgcctacga
     2281 cctcgccgcg cttctggact cgctcggagt gggtccaaca gccttggctg gtcattccat
     2341 gggaggttat atcgctctcg catttgcgaa gtcttttcca cagcgtgtga ccggtttggc
     2401 tttggtttcc tctcaagcag cctcagatac ccctgaacgt aaagaaggta gatataaaac
     2461 ggcggcagat gttgttcaaa aaggagttca gatcgtcgcc gatgcgatga cggataaact
     2521 ttcgcccaat cagtctgtgc gagattttgt ccgcccgttg attgcgaagc aaagcgtggc
     2581 tggtgtgacg ggggcactca aagccatggc agagcgtgac gatatgactt cgttcatagc
     2641 atcttatgct cagcctcttg tgttgattca cggagatgca gatgagttga taccgctcga
     2701 acgtgcgcgg gagattaagt cactggtctc gtctgcgcag ttggtggagt tggctggggt
     2761 ggggcatatg cccatgctgg aaatgccaaa cgaaacggct tcggcgttga aaaagctcaa
     2821 atagcaaaga aaatgcccat ccagctaacg gggatgggca tttttatttt gtagaaccag
     2881 cttcgggcta ggggtgaagc gcgtgattag cttgttggag ggaagccgtc gtttttttta
     2941 acttggacat atccagtgcg cctgtgttgc gtcgaatcaa tttcttgata ttcgcaacca
     3001 acacgccact gggcacgggt ttgacgagga aatcgtctgc accaacatcc aaaacgctcg
     3061 cgaccatgcg tggatcgttg attgcagaaa ggatcaggat cggcacggta cttgttttgc
     3121 gaatttcttt gcaaacctgc catccgtcta aatcaggcat cattaggtcg agtagaatga
     3181 cgtttggatt agcctcacgt gcaaggcgaa tgccttcttc gccaatgtta gttgtgagca
     3241 cttcaaaccc aaaggttttt agtaacatgc tcatcaactc tgtgatggca gcatcatcat
     3301 cgatcacaag gatttttgct gtaggtgtca taatatctag gcacattatc cctgatttga
     3361 ctgacaatgt actttgcaat gtattaacag gttcttttga ataagaatta gtaatttccc
     3421 tgattgacat ccactcttag catgattttt gtcaggtgct aattggactg tttttggtac
     3481 aatatttcac aatcaagata tagagtgaca atattactcg atcaggaggt cgaaatgtct
     3541 aaatgggttt atctctataa tgaggtgaag gaagttgaaa aattagccaa aggctcatgg
     3601 gatggtgtca aagccatcgt cggcggcaaa ggtgcgggct taatggatat gactcgcgcg
     3661 aatgtgccgg ttccaccttt ctttaccgtt actacagaag cgtgcaatgc atatcaaaaa
     3721 gctgggaaat tcccgcaagg aatgtgggag caggaattga aagcgctgaa agagattgag
     3781 cgcaaaaccg gcaaaaagtt cggtgatccg aaaaatccgc ttttggtctc gtgtcgctca
     3841 ggtgctaagt tctcaatgcc gggcatgatg aataccattc tcaatattgg tctgaccgat
     3901 atcgtagcag aaggtatgat caaggcaacg aacaacgacc ggtttgtgtg ggactcatat
     3961 cgacgtgtgg ttgaaatgtt cggaaccgtc gtgatggaca tcgaagacga agcctttgaa
     4021 catccattgg aagcatataa agagaaaaaa ggttataaac tcgatattga aatgagggcg
     4081 gaagattgga aagaagttgt ggggatttat aagcaagcgt acaagaaagc ctgtggtcag
     4141 gaatttcccc aagatcctta taaacaactc tcattagcaa ctgaagctgt attccgctcc
     4201 tggaatggaa aacgtgctgt cgcttaccgc cgcaaggaag gtatctctga tgcgctcgga
     4261 accgccgtca atatttgtac gatggtcttc gggaacatgg gcgatgactc tgctaccggt
     4321 gtggcattca cgcgtaaccc atccactggc gaaaagaaga tgatgggtga gtacctgctt
     4381 aatgcacaag gtgaagatgt ggtagctggt atccgcaata cggatgctat tgaaaacctc
     4441 aagaagtata tgcctaaggc atatgctcag ttcatggaaa ttaccgcccg ccttgaaaag
     4501 cactacaccg atatgcagga tgtggaattc accattgaac gcggcaagtt atggatgctc
     4561 cagactcgtg tgggtaaacg cacggccaaa gccgctgtga agatcgctgt ggatatggca
     4621 aatgaaggct tgatcaaca
//
LOCUS       DAOREF010000109         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10708_length_4625_cov_4.680525, whole genome shotgun sequence.
ACCESSION   DAOREF010000109 DAOREF010000000
VERSION     DAOREF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10708_length_4625_cov_4.680525"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..901)
                     /locus_tag="PKI33_03300"
     CDS             complement(320..901)
                     /locus_tag="PKI33_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36058.1"
                     /translation="MATACTSQPASSPTLSANDQAVIATSVAMTVMAENAASSENIST
                     PVVLDTPLPTFTVMAAPVELTSTATSTVQVPTVETVTTAGPCDKVLRVSDAGPLTHVR
                     FRNRTGGKIQLGVFLWQENSYGVCGYLPNNPINIGIAQSYTVNLPAGNYFIWAWITYK
                     NDDTGSVNGNFINNQTSGADTMEVIITKDGVSG"
     gene            complement(977..3496)
                     /locus_tag="PKI33_03305"
     CDS             complement(977..3496)
                     /locus_tag="PKI33_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNM36059.1"
                     /translation="MRLKKFHVLTWIVLLSIFVSACGGVLPIGGETAQPGTDATEPSI
                     EEIPSGNGPTVVTGSVEYTDLFFTMGVSEPIVILEDQGGFVARDRKFVIPVESQVLGQ
                     ITSDFYTSPFTYSLTLPAEPKGTLNDVDHDGQKDTGVMIFAVAYWTNTWGDPYLERRD
                     QGGGGWSSAYASTRVSDASDSFLEVYGGQYLVFAPDENQQFPSGFGADEKLFTDDDPL
                     MSLPAGWSMIDMDQKPFKIDRSNAPTLDLYEPASSALDDFSQMTYTEAFDAMLEKFRK
                     EYAYTEFKDVDWDAREKEFRPRFEEAEKNKDAHAYALALRDFVWSIPDTHVGMDTSAL
                     NDDFSADIAGGIGLALGETSDGQIVARYVTPGSPADAAGIKFGAEIVSLDGKPVDEVV
                     SAVTPWSSPFSNPEVKRLQQLRYATRFRAEKGQVEVSFVNPGGSEQSATLDVVSERDS
                     FSVTSFAFGQPETSLPVDFKILPSGYGYIKISSFSDNQVLMIQAWEYALDYFNQNEVP
                     GVILDMRNNNGGSGWMADQMAAYFFTKEVVAGTGSSYDEVTGDFYMDPASEAVMIPPR
                     EALQYTGPVVVMVGPACISACEFFSYNMTINDRATIVGQYPTSGAGGGVEAFMMPENT
                     YMQMTVSRQMDADGNIHIEGGGVAPDVRVPVTVESFQRQLAGDDVILEAAEAVIGQPK
                     GAGVTPSASPKIASPAEAEAAFSSGASFIDDKVKEQYSDTEVSVPGVLTYTAPLAKSE
                     TLIWAYYWCTTTKEVLDQNFSQINVQFELNGEVVPLDQFATAELPSGGNQCRIIYTAL
                     SDWKTGEHHLTTTVTFKTPINDGMGDYPAGDYVSEYSVFVP"
     gene            3537..3866
                     /locus_tag="PKI33_03310"
     CDS             3537..3866
                     /locus_tag="PKI33_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36060.1"
                     /translation="MNNIELEIQREFESAQRARANNNEGQARVCARRAAGIAIRDYLT
                     RKGTPLPSRSAHDLLNFIKDETPLPSDLKLVADHLTLRVTEEFKLPVDADLVAEAKAL
                     CDWLLKN"
     gene            complement(3921..4190)
                     /locus_tag="PKI33_03315"
     CDS             complement(3921..4190)
                     /locus_tag="PKI33_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36061.1"
                     /translation="MSENQTFTSQDTDLQFLESHLSRTLRRVPPPNGLVERLRGRVQM
                     PTRSEIRLRFSDWRRLFVVFGGVMSGMLVLVTLARAFYYLVGRKA"
     gene            complement(4200..>4625)
                     /locus_tag="PKI33_03320"
     CDS             complement(4200..>4625)
                     /locus_tag="PKI33_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNM36062.1"
                     /translation="VDEIKTFIEGVRSKTHVILTLETLEYAKRSGRVGTLSAALASVL
                     NVKPIAMLKDGIVDMVDRVRTRKGAIDRVLDLAKENVGDSPVHIGVVHARDEASAKSL
                     LEEAQKRFNVKESFLTDLSISLVINFGPGTVGLVLYPAE"
BASE COUNT         1309 a         1161 c         1013 g         1142 t
ORIGIN      
        1 gcgtagaaag ccctgtatct tcaaggctgt tgatttgcgg gggcgtaaac ccgccggaag
       61 aggatgggga aatttgtcct gtttgcttta ctaccatatt gtcacctagt cccaggcgga
      121 agaatttcaa gcgtgcgagc ctgattatac tgcttttcgt ttataactca gccgcgctga
      181 aactttcaac cttactcttt ctttacaatg aagttgaaag tatcttgtta tcatgtgact
      241 attttttaat catagtagaa aaggagacaa aaaaggcacc ggtcgtaatc ggtgcctttt
      301 ttgatataaa aaacttgttc taccctgaaa cgccatcttt cgtaataatc acttccatcg
      361 tatctgcacc ggaagtctga ttatttataa agttgccgtt tacagaacct gtatcatcat
      421 tcttataggt gatccatgcc cagataaaat aattacctgc tggcagattg acggtataac
      481 tttgagcaat accgatgttg attgggttgt tgggaagata cccacataca ccataagaat
      541 tttcctgcca caggaagaca cctaattgaa tttttccgcc tgtcctattc cgaaagcgga
      601 catgggtaag cggacccgca tcggaaaccc ttaatacctt gtcgcaaggt cctgcggtag
      661 taactgtttc tacagtggga acttgtaccg tagaagtggc tgtagacgtc agctccacag
      721 gagcagccat gaccgtgaaa gttggaagcg gagtgtccaa tactacaggt gtggaaatat
      781 tttctgaaga tgcggcattt tctgccataa ccgtcattgc aaccgaagtt gcaatgacag
      841 cttggtcatt agcagaaagt gtaggagaac ttgctggctg agaggtgcat gcagtagcca
      901 aaacagcaac cagaaggcta aataggaatc tcctcataga atattcgtgt ccaattttcg
      961 aaaagtccta tcaagtttat gggacaaaga cagaatactc cgaaacatag tcaccagcgg
     1021 ggtagtcacc cattccatcg ttaatcggag tcttgaaggt gacggtcgtt gtgagatgat
     1081 gctcgccggt tttccagtct gaaagagccg tgtagataat acgacactga tttccacccg
     1141 aaggcagttc tgcagtggca aattgatcca acggaactac ttccccattt aactcaaact
     1201 gaacattgat ctggctaaaa ttctggtcaa gaacttcttt tgtagtcgta caccagtagt
     1261 atgcccaaat caaagtctca gacttggcta gtggtgcagt ataggtcaat acgccgggga
     1321 cagagacttc ggtatcggaa tattgttcct tgactttatc atcaataaat gaagcaccgc
     1381 tcgagaaagc agcttctgct tcagccggtg aagcgatctt tggagaagca gatggggtaa
     1441 ccccagcacc tttgggttga ccaatcactg cttctgccgc ttctaggatg acatcatcgc
     1501 ccgccaactg acgctggaag gattcgacgg tgacaggcac acgtacatcc ggcgccactc
     1561 caccaccttc aatatggata ttgccatcgg catccatttg gcgcgaaacg gtcatctgca
     1621 tataggtatt ttccggcatc atgaacgctt ctacaccacc acctgcccca gaggtcggat
     1681 attgcccaac aatcgtggcg cggtcgttga tggtcatgtt ataggagaag aattcacagg
     1741 cactaataca ggctggacct accatcacca caaccggacc tgtatattgc aacgcctcac
     1801 gcggaggaat catcactgct tcactggcag ggtccatgta aaagtcgccg gtcacttcat
     1861 cgtagctaga gccggtacca gcaaccactt ctttagtaaa gaaataggct gccatctggt
     1921 ctgccatcca gccactacca ccattgttat tccgcatatc caagatcaca ccaggaactt
     1981 cattctggtt gaagtagtcc aaagcatatt cccaagcctg aatcatcaga acttgattat
     2041 ctgaaaaact gctgatcttg atgtatccat atccacttgg gagaatcttg aaatccacag
     2101 gcagggaggt ttcaggctgt ccgaaagcga aggaagtcac actgaagcta tcacgctccg
     2161 agacgacatc caacgttgca gactgttctg aaccaccggg gtttacaaac gaaacttcaa
     2221 cctgtccctt ctcagcacgg aaacgggtgg catagcgcag ttgctgcaaa cgcttcactt
     2281 cagggttact aaacggtgaa gaccaaggtg ttacagccga aacaacttca tccactggct
     2341 tgccatcaag agaaacgatt tcggcgccaa atttaatacc agccgcatct gcaggactac
     2401 ccggggtgac gtaacgcgcc acaatttgac cgtcactggt ttcacccaaa gcaagaccaa
     2461 tgccacctgc aatatcggca gaaaaatcat cgtttagtgc agaagtatcc atgcctacgt
     2521 gagtatctgg aatagaccaa acaaaatctc gcaatgctaa ggcataggca tgagcatctt
     2581 tattcttctc cgcctcttca aaacgcggac ggaactcttt ctcacgagcg tcccagtcaa
     2641 catctttgaa ttcggtatat gcatattcct tgcggaattt ttctagcata gcatcaaacg
     2701 cttcggtata ggtcatctgc gaaaaatcgt cgagggcaga gcttgccggt tcgtagagat
     2761 caagcgtcgg ggcgtttgag cggtcgatct taaatggctt ctgatccata tcgatcatag
     2821 accaccccgc aggcaaactc atcaacgggt catcatctgt aaatagtttt tcatctgcgc
     2881 caaagcctga agggaattgt tgattctcat ccggcgcaaa gaccaaatat tgaccaccat
     2941 acacttcaag gaacgaatca cttgcatcgc ttacacgagt tgaagcataa gcactagacc
     3001 aaccaccacc gccctgatcg cgacgttcaa gatatgggtc gccccatgtg ttcgtccaat
     3061 acgcaacggc aaagatcatt acgcctgtat ctttttgccc atcgtgatct acatcattca
     3121 atgtcccttt aggctccgca ggaagggtta gactataggt aaaaggtgaa gtataaaaat
     3181 cagatgtgat ctgcccaagt acctgtgatt caacaggaat cacaaatttg cgatcgcggg
     3241 caacaaaacc gccctgatct tccaaaataa caatcggctc agaaacgccc attgtgaaaa
     3301 acaagtctgt atattccaca ctaccggtca ccacggttgg accattacca ctgggaattt
     3361 cctcaatgga aggttcggtt gcatctgtgc ctggctgtgc tgtttcgcct ccaatcggca
     3421 aaaccccgcc acaagcgcta acgaatatac ttaacaaaac gatccaagtt aatacgtgaa
     3481 actttttaag cctcatatgc cagcctcctg gaaatttcaa gaattataat cccagcatga
     3541 acaacatcga actggaaatc caacgtgaat ttgaatctgc tcaacgagcc agagccaata
     3601 ataatgaagg gcaagctcgt gtctgcgcgc ggcgtgcagc tggcattgcc attcgggatt
     3661 atctgacccg caaagggact cccctgccga gccgttctgc ccatgatctg ctgaacttca
     3721 tcaaggatga aacgcctctt ccctccgacc tgaaattggt cgctgaccat ctaactttgc
     3781 gcgtcactga agaattcaaa ctccctgtcg atgccgatct ggtggctgaa gctaaagctt
     3841 tatgtgactg gttactgaaa aattaaaaga aaaactcctg aaagatatct ttcaggagtt
     3901 ttttgtagaa aatgcagaag ttacgctttg cgcccaacca gataataaaa cgcacgcgcc
     3961 agtgtaacca agaccaacat accagacata acgccaccaa acacaacgaa caatctacgc
     4021 caatctgaaa aacgcaatcg aatttcagaa cgtgtgggca tttgcacgcg tccgcgcagg
     4081 cgttcgacca agccgttcgg aggcggcacc cgtctcaagg tccgcgagag atgcgattca
     4141 agaaattgca ggtccgtatc ttgggaagtg aaagtctggt tttcgctcat agaatttaat
     4201 tactcagcag ggtacaacac caatccaacc gttccaggtc cgaaattgat tacaagagag
     4261 atggaaaggt cggtcaaaaa gctttctttg acgttgaagc gtttctgcgc ttcctctaac
     4321 agagacttgg cagaagcctc atcacgcgca tggacaacac cgatatgaac cgggctatcg
     4381 cccacatttt cttttgcaag gtcgagcacg cgatcaatgg cacctttacg agtgcgcaca
     4441 cgatcgacca tatccacaat gccatctttc aacatagcaa tcggtttgac attcaatacc
     4501 gaagcaagtg ctgctgaaag ggtgcctaca cgcccactac gtttggcata ttcaagcgtc
     4561 tcaagcgtga gaatgacgtg ggtcttacta cgcacacctt caataaaagt ctttatttca
     4621 tctac
//
LOCUS       DAOREF010000110         4618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10739_length_4618_cov_4.090730, whole genome shotgun sequence.
ACCESSION   DAOREF010000110 DAOREF010000000
VERSION     DAOREF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_4618_cov_4.090730"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKI33_03325"
     CDS             <1..622
                     /locus_tag="PKI33_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNM36063.1"
                     /translation="HEIQREPCVTRDNLILNTGFYISWRIDNPALSLTRVSDLENSMR
                     LLSTAMVRAAIAEFNMQDALSRRRALNTLIRTRIVQRANDWGVAVNDTEIRELQPTDG
                     VLRQIENRFNATLEGDAALLRSDAHVESLRRFMALEPGVNPRVLTLKYLDTLEKIGEG
                     ASTKYIIPMELFTMLQEVLRGQINGNGTNVGNGNNPPEQLPGGPIQ"
     gene            complement(641..871)
                     /locus_tag="PKI33_03330"
     CDS             complement(641..871)
                     /locus_tag="PKI33_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36064.1"
                     /translation="MSNVSDIKPWFREDIARTLMSIYFASAANNSQRTAENENYRVGF
                     AAALSSVALAVGVNPESFLAPEDIQRLRSTSR"
     gene            1183..2601
                     /locus_tag="PKI33_03335"
     CDS             1183..2601
                     /locus_tag="PKI33_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36065.1"
                     /translation="MKSAFSGIGSFLQTPFGVIAAGTVVFHGILFAVGFNYTVVTLVI
                     AVVVLGVNLFAANYIADKLVYFYSQFVLPVQTPKDRQEIYTRVSGFDGSRGPILFVKN
                     GRVIKHEGETEKKGPGVIVLDTASAIVTQTETEIIGAAGPGIHFTKGREKITRGEGVD
                     LRPQWQFIGPLASDQPFLNPPSPDSKKYNELQARRQQTAGQTRDGFEISPTISIKFRI
                     KQPEEKTPSESGVVSHYGYNATAVLHAVTREVIELGTSDNKRTRMEWYRLPAHLVVNL
                     WREYVRKFKLEDLFKAEGVSGLQTIEEMINRRVKRPEVVAMDDTGIPTGEALPSLEYQ
                     QLIERGLEVIDVRIHNVLFDPAIEEQTIKNWNNEWTKIAKREEDLLNEREKLIETAAH
                     NEAVKRFARLASLKFENPIAMPADAYTTLQALIEPVKETLVIESRANSQMETDIKKLD
                     EIWKWLLVSKLDKSMHRKEDES"
     gene            2598..4187
                     /locus_tag="PKI33_03340"
     CDS             2598..4187
                     /locus_tag="PKI33_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36066.1"
                     /translation="MNRFRRRRDEMIPRIFALTDEAVEYRSQIRNYARIVVWAVLSGF
                     TLILFFFFAETTIQRLTVLGLSLLIYLPLFSAAQTVARHKAANYLADIYEIEDETIAD
                     SFLEEVIFGEGTEKITINEGKISERDEESPLIMIGGPGYIQVNLDSVALLERVDGTPE
                     IIHPRGKPWKLGSFERIREIGKHDEPGKREYAIINLRDQFVRGLSVRSRTKDGIPLEA
                     QDIKVLFSILRKPKEEAPENDPYHFDETAVHSLVYNQTIISPSPAKTAGVSFPWDTTV
                     IPLITSEMENLITSRNLSEILASISHKEIDDLNEGEATNKQIRLEMTGEQTVPTNSNS
                     PSAPNFESRSKITAQFFSPQFKAKAAKLGVSIHWIDIGTWQLPSEMILEELKSGWKMM
                     RENVRRRNDVNRLAKRQEMKELRELVNHVIVSSFTKANNANDNRKLSDDEFSKLSKII
                     EENPNIAFSPALQQKMAQDVTLKKDAALEILKAFRKEFIAARELIQKENRSSIDKQAE
                     IARIDKALRDIDYHVFHYIKG"
     gene            4190..>4618
                     /locus_tag="PKI33_03345"
     CDS             4190..>4618
                     /locus_tag="PKI33_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNM36067.1"
                     /translation="MKHPHIQLSVEVSRALTMGAPIVALESTVITHGLPQPQNLELAR
                     NMEQQVRDAKATPATVAVLDGKIRLGISDEELVRLSESQSTLKVSHRDFATAIVKKVN
                     GGTTVAGTMLAANMSGIKVFATGGIGGVHKESAFDISTDLK"
BASE COUNT         1339 a         1109 c         1070 g         1100 t
ORIGIN      
        1 acatgaaatc caaagagagc cttgtgtcac tcgcgataac ttaatcttga atacaggttt
       61 ctatatctcg tggcgaattg ataaccccgc attaagcctt actcgcgttt cagatttaga
      121 aaactccatg aggcttcttt cgacagcaat ggtacgtgca gccattgcag agtttaacat
      181 gcaagatgcc ttaagcagaa gaagagcatt aaacacatta atcagaacac gaatcgtaca
      241 aagagcaaat gattggggtg tcgctgttaa tgacactgaa attcgagagc ttcaaccgac
      301 agatggagtg ttacgccaga tcgaaaatcg atttaatgcg acattagaag gtgatgctgc
      361 tctcctaagg tcagatgctc atgtagagtc attaagaagg tttatggctc ttgaacctgg
      421 tgtcaatcca cgagtcttaa ctttaaaata cctcgacaca ctcgaaaaaa ttggcgaagg
      481 tgcatccaca aaatatatta ttcctatgga gctttttacc atgctgcaag aggtgcttcg
      541 tggacaaata aatggaaacg gcactaatgt cggaaacgga aataatccac ctgaacaatt
      601 gcccggtgga cctattcaat aatctataaa caataataat ttatctggat gtgcttctaa
      661 gtctttggat atcttcggga gcgaggaagc tttcgggatt cacccccact gctaatgcca
      721 cgctggaaag cgctgcggca aaacccacgc gataattttc attttctgcg gtacgttgtg
      781 aattatttgc agcggacgcg aaatatatac tcataagagt tcgggctata tcttcacgaa
      841 accatggttt gatatcactt acattagaca ttgtcttact ccttggcgtt acataaaagc
      901 aactagataa attatagaac ataagttcta tttaaacaag aacgagggta ttgtacaccc
      961 acaatataca agtagcgcat ttacaatatg ctttcaatga atcaccacac ttaacacata
     1021 ttttaggtgt tgggatatgt ctcccggctt gcaaaacgcg ggaaatatat cacaatgtca
     1081 cattgctaca gggtcatcca ttcgggggat tcgaaaattc aaagcaacct gttatatttc
     1141 agcccgccag attggcaaat tttgatttag aatcatgcga caatgaagag cgcctttagc
     1201 ggaatcggtt catttttaca gacgcctttt ggggttatag cagcagggac ggtcgtcttt
     1261 catggcattt tgtttgcggt cggttttaac tacaccgttg tcactcttgt aatcgcggtt
     1321 gtggtcttgg gcgtaaacct ttttgccgcg aactatatcg ccgataaatt ggtctatttc
     1381 tattctcaat ttgtgctccc cgttcaaaca ccaaaagacc ggcaggaaat ctacacccgt
     1441 gtcagcggat ttgacggcag ccgtggaccg atcctcttcg tcaagaatgg acgggtgatc
     1501 aagcacgaag gcgagaccga gaaaaaaggt ccgggtgtga tcgtgctcga taccgccagc
     1561 gccattgtca cccaaaccga aacagaaatc atcggcgcgg caggtcccgg catccacttc
     1621 acgaagggac gtgaaaagat cacccgtggt gaaggtgtgg atttgcgccc gcaatggcag
     1681 tttattggtc cgttggcgag tgaccaaccg tttctcaacc cgccttcgcc tgattcgaaa
     1741 aaatacaacg agttgcaggc tcggcgtcaa caaaccgccg ggcaaacgcg cgatgggttt
     1801 gaaatatccc ccactatcag tatcaagttc cgtattaagc agccagaaga aaagactcct
     1861 tctgaaagcg gcgtcgtttc tcattatggc tataacgcca ccgccgtcct ccatgcggtg
     1921 acacgcgaag tcattgaatt gggaacttcg gataacaaac gtactcgcat ggaatggtat
     1981 cgccttccgg ctcaccttgt ggtgaatttg tggcgcgagt atgtgcgcaa gttcaagctg
     2041 gaagatttgt tcaaagccga aggtgtcagc gggttacaaa ccatcgaaga aatgatcaat
     2101 cgtcgggtga aaagacccga agtagtcgcc atggacgata ccggaattcc aactggtgaa
     2161 gcattgccca gcctcgaata tcagcaactc atcgaacgcg gtcttgaggt gatcgatgtg
     2221 cggattcaca atgtgttgtt cgaccctgcc attgaagaac agaccatcaa gaattggaac
     2281 aacgagtgga ccaagatcgc caagcgtgaa gaagatttat tgaatgaacg cgagaagttg
     2341 atcgagacgg cggcccataa tgaggctgtc aaacgttttg cgcggctcgc ttcgctaaag
     2401 ttcgagaatc cgattgccat gccggctgat gcctacacaa cattacaagc tctgatcgaa
     2461 ccagtaaaag agaccttggt tattgaaagc cgcgccaaca gtcagatgga aactgacatc
     2521 aagaaattag atgaaatttg gaagtggttg ttggtaagca agttggataa atccatgcat
     2581 cgcaaagagg acgaatcatg aatcgattcc gccgtcgacg cgatgaaatg attccgcgca
     2641 tctttgctct caccgatgag gctgtcgagt atcgaagcca gattcgcaac tacgcccgca
     2701 ttgtggtgtg ggcggtatta agcggattca cactgatctt gttctttttc tttgccgaaa
     2761 ctacgataca acgactgacg gtgttgggct taagtctctt gatctaccta cctttgttta
     2821 gtgcagcgca aaccgttgca agacataaag ccgccaatta tttggcagat atttatgaaa
     2881 tagaagatga aacgatcgcc gatagttttc ttgaagaagt gatcttcggc gaagggacag
     2941 agaaaatcac gatcaacgag ggcaaaatct cagaaagaga tgaagagtcg cccctcatca
     3001 tgatcggcgg acccggctat attcaggtca acttggatag tgtggcgctg ctggaacgcg
     3061 tagatggcac tcctgagatc attcacccac gcggcaagcc gtggaaactg ggcagttttg
     3121 agcgcatccg tgagatcggt aaacacgatg agcccggcaa acgagaatat gccatcatca
     3181 acctgcgcga ccaattcgtg cgcggacttt ccgtccgctc gcgcaccaaa gacggtattc
     3241 cgcttgaagc gcaggatatc aaagtgcttt tcagcatttt gcgcaagccc aaagaagaag
     3301 cgcctgaaaa tgacccgtat catttcgatg aaaccgcggt ccattccttg gtgtataacc
     3361 aaactatcat ttcaccttcg cctgcaaaaa ccgctggcgt ttctttccca tgggatacga
     3421 ccgttattcc gctcatcaca agcgaaatgg aaaacctcat cacttcgcgc aacttgagcg
     3481 aaattcttgc cagtatcagc cacaaagaaa ttgatgacct caacgaaggt gaagcgacca
     3541 acaaacaaat tcgtctcgaa atgacgggcg agcaaacggt tccaaccaac agcaattctc
     3601 ccagtgcacc aaactttgag tcacgttcaa aaatcacagc gcaatttttc agtccgcagt
     3661 tcaaagccaa agccgccaaa ctcggcgtca gcattcattg gatcgacatt ggcacatggc
     3721 agcttccttc tgaaatgatt ctcgaagaat taaaaagcgg ttggaagatg atgcgtgaaa
     3781 atgtccgccg gcgcaacgat gtcaaccgac ttgcgaaacg gcaggaaatg aaagagttgc
     3841 gcgaactggt aaatcatgtc attgtttcca gtttcaccaa agcgaacaat gccaacgaca
     3901 accgcaaact ttcagacgat gaattttcaa agttatccaa gatcattgag gaaaacccga
     3961 acatcgcctt cagccctgcc ttgcagcaaa agatggcaca agatgtcacc ctcaaaaaag
     4021 atgccgcgct cgaaattctc aaagccttcc gcaaagaatt tatcgcagcc cgcgaactca
     4081 tccaaaaaga gaaccgctct tccatagaca aacaagccga aatcgcaagg atcgataaag
     4141 ccctgcgcga catcgactat cacgtattcc attacatcaa aggttaagaa tgaagcatcc
     4201 ccacatccaa ctttctgttg aagtttctcg cgcgctcacg atgggcgcgc ctattgtcgc
     4261 tctcgaatcg acagtgatca cgcacggttt accccaaccg caaaatttgg aactcgcccg
     4321 caacatggag cagcaagtcc gcgatgcaaa ggcgacgcca gccactgtgg ctgtattgga
     4381 tggaaagatc cgcctcggaa tctcagacga ggaactggtg cggctttctg aaagtcaatc
     4441 cacgctgaaa gtgagtcacc gcgattttgc cactgccatt gtcaaaaaag tgaacggcgg
     4501 taccaccgtc gccgggacaa tgctcgccgc caacatgagc ggtatcaaag tttttgctac
     4561 aggtggtatc ggaggcgttc ataaagaatc tgctttcgat atctccaccg atttaaaa
//
LOCUS       DAOREF010000111         4604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10791_length_4604_cov_4.227962, whole genome shotgun sequence.
ACCESSION   DAOREF010000111 DAOREF010000000
VERSION     DAOREF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10791_length_4604_cov_4.227962"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PKI33_03350"
     CDS             <1..1104
                     /locus_tag="PKI33_03350"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNM36068.1"
                     /translation="GYEVQTVRVATVSFAKLLGEKHIDELPKYAEHFTQVMKQNGVVY
                     AALGPALPELPRSYRVIPEAISASDGVFFSGVMADAQTIHMAQVKACAEIIVKSAPLD
                     PNGFANLHFTALANVGANAPFFPGAYHTSDQPAFGIAVECADMAVDAFANAQSIEEGR
                     QWLVSEMTKHGQAITNIANGLQIEFKGIDFSLAPFPDDAHSLGKAVENMGVPKIGLHG
                     SLAAAAILTDAIERADFPHIGFNGFMQPILEDSILAKRAAEGALTVKDALLYSAVCGT
                     GLDTVPLAGDVTADELTPLLLDLCALALRLNKPLTARLMPVPGKKAGDATTFDFAFFA
                     NGRVMALESQPLSLPFNSNESFSLKPRAERNRP"
     gene            1194..2870
                     /locus_tag="PKI33_03355"
     CDS             1194..2870
                     /locus_tag="PKI33_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HNM36069.1"
                     /translation="MKRNILIIGGLVFVAIVAFFVYKAVAPSKPVNVQLLALNDFHGN
                     LLPPSGSSGQVDGVDAGGVEYLATHIDTLRGENPNTVVVSAGDLVGASPLLSALFHDE
                     PTIEAFNLIGLDFNAVGNHEFDEGWAELKRLQEGGCHPVDGCLDGDDFSGAEFKFLAA
                     NVINKETGEPIFPAYEVREFEGVKIAFIGMTLKGTPSIVTPSGVADLEFLDEAETVNA
                     LIPELQAQGIETIVVLVHEGARQNGDAPTANTCQDIDGPIVDIVSRFDDAVDVVISGH
                     THQAYNCLLDGKLLTSAMSFGRLVTQIHLTIDPKTGDVTTMEANNLVVTRDVEKASLL
                     TALIERYNKVAEPLANHEVGTITADILREPNDAGEMALGDVIADSQLFATSASDMGNA
                     VIAFMNPGGIRTDLVYADSVGEGDGTVTFGEIFTVQPFNNNLVTLTLTGEQIDALLEQ
                     QFVDCGQTENPRILQVSEGFSYTWKASGAPCEKVDPATIFLNDEPIDPAKEYRVTVNS
                     YLADGGDRFKVLTEGTDRLVGVMDLDALEAYMLQASPVEPGAQDRIKVIP"
     gene            complement(2911..3468)
                     /locus_tag="PKI33_03360"
     CDS             complement(2911..3468)
                     /locus_tag="PKI33_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNM36070.1"
                     /translation="MSEELVAFFKVLSDANRLKIIGLLAHQPYSVEELAALLGLKPST
                     VSHHLSKLSKVGLVSAKTESYYNVYQLDQKALEEKSRSLFSQDKMTASVADVDVDAYD
                     RKVIGDYTRKDGSLKTIPAQRKKLDAVLRHIVKAFKVGKRYSEKQVNEILSTYHDDTA
                     SLRRELVGAKLMQREGGGGEYWRDE"
     gene            complement(3470..3835)
                     /locus_tag="PKI33_03365"
     CDS             complement(3470..3835)
                     /locus_tag="PKI33_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNM36071.1"
                     /translation="MKTRKELNREYAERVKPAGIYQVKNTVSGKMFLGSSLNLEGPLN
                     RHRFMLKIGSHTNKALQNDWNQLGADAFAFEILEEVKMKDDPNFNLQDELTLLEMIWL
                     EKLQPVENGYNLNARIREA"
     gene            complement(3832..4419)
                     /locus_tag="PKI33_03370"
     CDS             complement(3832..4419)
                     /locus_tag="PKI33_03370"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HNM36072.1"
                     /translation="MNEMLDYVKALSDPERLRIIGLLSQGNATREEIATRLNLSPKDS
                     LTHLGFLEFVGVVTQTDGIFALNNDKLATLGKEKLFNGGNKFIPPEDMDEASKKILRD
                     FLNADGTIRQVPEQKKIGPILDYLIKNFEFDRTYTEREVNIIIKQFNEDTAGLRRDLV
                     DAGLMDRESDGSKYWRVDLSRKRLKLTDDSTWTIK"
BASE COUNT         1151 a         1077 c         1129 g         1247 t
ORIGIN      
        1 gggtatgaag tgcaaaccgt gcgtgtggcg acagtttcat ttgcaaagct gctgggggaa
       61 aaacatattg atgagttacc gaaatatgct gagcatttta cacaggtcat gaaacagaat
      121 ggcgttgtgt atgcggcgtt gggtccagct ttgccggaat tgccgcgcag ttaccgggtc
      181 ataccggagg cgatttcagc aagtgatggc gtcttcttca gcggcgtcat ggcagatgcg
      241 cagacgatcc acatggcaca agtcaaggca tgtgcagaga tcatcgtcaa gtctgcaccg
      301 ttagacccga atggatttgc caacctgcat ttcacggcac tcgcaaatgt cggtgcgaat
      361 gcgccattct tcccgggcgc gtatcatacg tctgaccagc ctgcgtttgg aattgccgtc
      421 gaatgtgctg atatggcagt ggatgccttt gcaaacgccc aatccattga agaagggcgt
      481 caatggctcg tttcagaaat gaccaaacat ggtcaagcca ttacgaacat cgcaaacggt
      541 ttacaaatcg aattcaaagg catcgacttc tcgcttgcac ccttccctga tgatgcacat
      601 tcgctgggca aagccgtaga gaatatgggc gtgccgaaga tcggtttgca tggttcgctt
      661 gcagcagcag caattctcac cgatgccatt gagcgggcag actttccgca tattgggttc
      721 aatggtttca tgcaacccat tctcgaagat tctattttgg caaagcgtgc cgccgaaggt
      781 gcgctgaccg tgaaggatgc tttgctttat tccgccgtat gcggcacagg tttggatact
      841 gttccactgg ctggggatgt aaccgcagac gaactcacgc ctcttctctt ggacctgtgt
      901 gctttggcgc ttcgattgaa caaacccctc accgctaggc tgatgcccgt cccaggtaaa
      961 aaggcgggag acgcaaccac ctttgacttc gctttcttcg ccaacggacg tgtgatggca
     1021 ttagagtcac agccgctgag cttgcctttc aatagcaatg agtcattttc gctgaagcca
     1081 agggcagaga gaaaccgtcc ctgaagaaaa ggttgaagag gggctgccag ttttcggtta
     1141 agaatgaagc gggttttcat cctgtttcaa atagttcaag aggaaaataa tcaatgaaaa
     1201 gaaatatcct aattatcggt ggattggttt ttgtcgcaat cgttgcgttt tttgtatata
     1261 aagccgtggc accatctaag cctgtaaatg tacaactgct ggcattgaat gactttcatg
     1321 gaaatttgtt gccgccttct ggctcttcgg gtcaggtgga cggtgtggat gcaggcggcg
     1381 tagagtatct ggcaacgcat atcgatacgt tgcgcggtga aaacccaaat acggttgtgg
     1441 tttctgctgg tgatctggtg ggagccagcc cgctgctttc tgcgctcttt cacgatgaac
     1501 cgaccattga agcctttaat ttgatcgggt tggatttcaa tgccgttggc aaccatgagt
     1561 ttgacgaagg ctgggcggag ttaaaacgtt tgcaagaggg cggttgtcac cctgtggatg
     1621 gatgtttgga tggcgatgat ttttcaggag ccgagtttaa attccttgcg gcgaatgtta
     1681 tcaacaaaga gactggcgag ccgatctttc cagcctatga ggtgcgcgag ttcgaaggcg
     1741 taaaaatcgc tttcattggc atgacgctca aaggaacgcc aagcatcgtc acgccttctg
     1801 gcgttgcaga tttggaattt ctggatgagg ctgaaacggt gaatgcactt atccctgaat
     1861 tgcaagcgca agggattgag accattgtag ttttggtgca tgagggcgca cggcaaaatg
     1921 gagatgctcc cactgcaaat acctgtcagg atattgacgg accgatcgtg gatattgtca
     1981 gccgctttga tgatgctgtg gatgtggtca ttagcggaca tacacatcag gcatataact
     2041 gcttgctgga tggaaaactg cttaccagcg ccatgtcgtt tgggcggctc gtgacgcaaa
     2101 ttcatttgac cattgacccc aagaccggtg atgtaaccac catggaagcc aataatttgg
     2161 tcgtgacccg cgatgtggaa aaggcgtcat tactaactgc attgatcgaa cgctacaaca
     2221 aagttgcaga accgcttgcc aatcatgagg tgggcactat caccgcagat attctgcgcg
     2281 aacccaatga tgcaggtgaa atggcattag gcgatgtgat tgcagattca cagctctttg
     2341 ccacaagtgc ctctgatatg ggcaatgccg tgattgcctt tatgaacccg ggcggcatcc
     2401 gcacggattt ggtatatgcc gattctgtcg gtgaagggga tggcacggtt acttttggtg
     2461 agattttcac cgttcagccg ttcaacaaca atctggttac gctcactcta accggtgaac
     2521 agatcgatgc tttgcttgaa cagcaatttg tagattgcgg acagacggag aatccgcgca
     2581 ttttgcaggt ctctgaagga ttctcgtata catggaaagc gtctggcgct ccatgcgaaa
     2641 aagtggaccc tgctacaatc tttttaaatg atgaaccaat tgaccccgca aaagaatatc
     2701 gtgtcacggt caactcgtat ttagcagatg gcggtgaccg cttcaaagtg ttgacagaag
     2761 gaacagatcg cctcgtaggt gtaatggacc ttgatgcttt ggaagcctac atgctccaag
     2821 cttcaccagt agaacccggg gcacaagatc gaattaaggt tattccataa acacgaataa
     2881 aaagagactc tggcaagcca gagtctcttt ttattcgtct cgccaatact ctccgccacc
     2941 gccctcacgc tgcatgagct ttgctcccac cagttcacgg cgtaaagaag ctgtgtcatc
     3001 atgataggtg cttagaatct cattgacctg cttttcgcta taccgtttac caactttgaa
     3061 agcttttacg atgtgacgca gaaccgcatc caacttctta cgctgagcag ggatggtttt
     3121 caagctacca tctttccgag tgtaatcacc gattacttta cgatcataag catccacgtc
     3181 cacatcggca acagaggcgg tcattttgtc ttgagaaaac aggctgcgag atttttcttc
     3241 cagcgccttc tggtcaagtt gataaacgtt gtaataactt tccgtctttg cgctcaccaa
     3301 gccaaccttt gaaagcttgg acaagtgatg cgagacagta gaaggtttca gccccaataa
     3361 agcagcgagt tcttccacac tatacggctg atgcgcaagc agtccaatga tcttgaggcg
     3421 attcgcatcc gaaaggactt tgaaaaaggc gaccaattct tcgctcatat cacgcctccc
     3481 ggattctggc attcaggttg tagccatttt caacaggctg aagcttctca agccagatca
     3541 tttccagcag agttaactca tcttgaaggt tgaaattcgg gtcgtctttc atctttactt
     3601 cttcaaggat ttcaaaggca aaggcatctg cacctaattg attccaatca ttttgaagag
     3661 ccttattggt atggcttccg atcttaagca tgaagcgatg gcggttcaac ggaccttcaa
     3721 ggtttaggct gctgccaaga aacatcttac cgcttactgt atttttcacc tgataaatcc
     3781 cagccggctt aacacgttct gcatattcgc gattgagttc tttacgggtt ttcatttgat
     3841 cgtccatgtt gagtcatctg tcaacttaag gcgttttcgt gaaagatcaa ctctccaata
     3901 cttcgagcca tcactttcac ggtccataag ccctgcatcc accaaatccc gccgcagacc
     3961 ggcagtatct tcattgaatt gtttgatgat gatgttaact tctcgttctg tataggttct
     4021 atcaaactca aagtttttga ttaagtaatc caatataggt ccaatctttt tttgctctgg
     4081 cacctgacga attgttccat ctgcatttaa aaaatctcgc agaatctttt ttgaagcctc
     4141 atccatatct tctggcggaa taaatttatt accgccattg aatagctttt cttttccaag
     4201 cgtggcgagt ttatcgttat taagcgcaaa aataccgtca gtttgtgtaa caacacccac
     4261 aaattcaaga aagcccaaat gggttaatga atctttgggc gagaggttta accgcgtcgc
     4321 aatttcttcg cgcgtggcat ttccctgaga aagcagacca atgatccgca agcgttccgg
     4381 gtctgataag gctttgacat aatccagcat ctcattcata tttcacttcc gtagatttat
     4441 ttcgacaatt atcgaattag attttaggag ggaatccacg gtttgtcaag ggataaggtc
     4501 cgctaccagc gtgagagcgg cttggtctgt agttccaagc ctgagcgccg cccgaagcgg
     4561 actctctgag tcggtgttat acatggtggt atgcaatgcc cgca
//
LOCUS       DAOREF010000112         4566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_10916_length_4566_cov_3.539127, whole genome shotgun sequence.
ACCESSION   DAOREF010000112 DAOREF010000000
VERSION     DAOREF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10916_length_4566_cov_3.539127"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PKI33_03375"
     CDS             complement(<1..992)
                     /locus_tag="PKI33_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36073.1"
                     /translation="MLNIDEEVKKVLNEVGPGRMSSTAYDTAWVARLGEIDNDLSDQA
                     VNWLLENQLPDGSWGASDVFYYHDRVISTLIAMIALKEHGGNSHHQAQVEKGVEALER
                     ITSDGEHSFASDLNGATVAFEMVAPILLAEAKELGIIKQQGERILGRLSNMRQEKMRK
                     LAGYKINRYVTPAFSAEMAGIDGQHMLEVENLQEANGSIGHSPSATAYFALRVKPGDA
                     KAIDYIKSSVAKNGGAPDLYPFDVYERAWVLWNLSLTDYFEGDKRSFANPILNYLAAA
                     WKPGQGVGFSKDYSVIDGDDTIITCELFNKFGYPTDLNAILSFEEEEYFRCYQL"
     gene            complement(1001..1915)
                     /locus_tag="PKI33_03380"
     CDS             complement(1001..1915)
                     /locus_tag="PKI33_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36074.1"
                     /translation="MSPMSLEAFLALPTEELANLVRGDRVKTCVFPFNGTRRWFLLER
                     PSSTGGDLAKQYIELTTQGYINTYKLVFDHGIDNIVAPAFGGELLVNRGEEYMRQIGT
                     SLSLLAIHPDFVNFYKEYDVRVHFYGEYRKQFKDTSYAYIIDQFDEVTRQTADHTSRA
                     LFYGVFAEDATMTTAELAIQHVQQTGTHPTRRDLIERYYGEFIEKADIFIGYEKFNVF
                     DYPMLGLGEESLYFTVAPSLYMNDRQLRNILYDFLYLRPVQDPDYFSMPQADFEAMRR
                     YYEANREVTFGIGEMRGGIWYATPHLKK"
     gene            complement(1919..2791)
                     /locus_tag="PKI33_03385"
     CDS             complement(1919..2791)
                     /locus_tag="PKI33_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNM36075.1"
                     /translation="MKRIIVAGIYRSGTSLITELVHRWGAYAGREEDIFKDEFGYMEH
                     FALQKLNDELLNNNSRVPTPVETLLEKAEDAGLRERALSILAEMDEEAQANHAAAWVW
                     KDPRLPLVLPFWADVWGDVIYVIPVRHPVETIFSAAKMDGLDPEQLPLSAGLAYWQFC
                     MLNLLQCTANTPRKLFMAYDQLTKDPQRECERLCRFLDAQCEVDSDPSDRIRSMTEQV
                     AAGERHFNHPRSLAEVETSTREQRALYDFLRVKTLYPDEPFHKDDFAMYPGWFEYLSA
                     MDMLFSAGDAGQEA"
     gene            3332..>4566
                     /locus_tag="PKI33_03390"
     CDS             3332..>4566
                     /locus_tag="PKI33_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNM36076.1"
                     /translation="MTLLDQFNKWKETTLKKSLDKFKERKGRFETSAGIEVPRLAIPD
                     GNEERGDLAPTVADENYVNKLGFPGEFPYTRGVQPTMYRSRFWTMRQYAGFSTAEESN
                     KRYRYLLEQGQTGLSVAFDLPTQIGYDADDPIAQGEVGKVGVSISSIKDMEQLFDQIP
                     LDKVSTSMTINAPAGVLLAMYIAVAKRQGADMRELRGTIQNDILKEYVARGTYIFPPA
                     PSMRLITDIFQFCSKEVPYWNTISISGYHIREAGSTAVQEVAFTLANGIAYVEAALKA
                     GLNVDDFAGQLSFFFNAHNNLLEEVAKFRAARRMWAKIMRERFKAQKPSSWQLRFHTQ
                     TAGSTLTAQQPENNVVRVTLQALSAVLGGTQSLHTNSMDEALWLPTEKSVRVALRTQQ
                     IIAHESGVADSIDPLAGSYL"
BASE COUNT         1182 a         1129 c         1112 g         1143 t
ORIGIN      
        1 tccaattgat aacagcggaa gtactcttct tcttcaaaag acaatatggc attaagatcg
       61 gttggatagc caaatttatt aaacaactca caagtaatga tggtgtcatc gccatcaata
      121 acactgtaat ccttagaaaa accaaccccc tgccccggtt tccatgctgc tgcaaggtag
      181 ttcaaaatag ggttagcaaa agaccgctta tcgccttcga aataatcggt tagagataaa
      241 ttccataaga cccatgcccg ttcatacaca tcaaacggat agagatcagg cgcgccgccg
      301 tttttcgcca cagagctctt tatataatct atcgcttttg catctccggg ttttacccgc
      361 aaagcaaaat aagctgtggc agatgggcta tgcccaatgg aaccattcgc ttcctgtaag
      421 ttctcaacct caagcatatg ttgaccatct ataccagcca tctcagccga aaaggctgga
      481 gtgacatagc ggttgatctt atacccggca agtttgcgca tcttctcttg ccgcatgttg
      541 ctcaggcgac ctagaattcg ctcgccctgt tgcttgataa tgcctagctc cttggcttct
      601 gcaagcaaga tcggagcgac catttcaaaa gcaactgtgg caccgtttag gtctgaagcg
      661 aagctgtgct ctccatcaga agtgatacgc tctaatgctt ctacaccctt ttcaacttga
      721 gcttgatgat gagaattccc accatgttcc ttaagcgcaa tcatggcaat cagcgtgcta
      781 atgacacggt cgtgataata gaacacgtcg ctggcgcccc aactaccgtc tggcaattga
      841 ttctcaagca gccagttgac agcttggtcg cttaagtcgt tgtcgatttc gcctaagcgt
      901 gcgacccaag cggtgtcgta ggcagtgcta ctcattcgtc cgggtcccac ctcgttcagt
      961 acttttttta cttcttcgtc gatgttaagc atgatgaact ctacttttta aggtgtgggg
     1021 tggcgtacca aattcctccg cgcatctcac ctattccaaa ggtgacctcg cggttcgctt
     1081 cataatatct gcgcatggct tcaaaatctg cttggggcat gctgaaataa tcagggtctt
     1141 gcaccggacg cagataaaga aaatcataga gaatatttct caactgacga tcgttcatat
     1201 atagagaagg agccaccgta aagtacaaac tctcttctcc caagccaagc atgggatagt
     1261 cgaagacgtt gaatttttcg tagccgatga aaatatccgc tttttcaatg aattcgccgt
     1321 aataacgctc gatcaggtcg cgccgggtgg ggtgcgtacc cgtttgttgg acgtgttgaa
     1381 tggcaagctc tgcggttgtc atggtggcat cttcggcaaa gaccccatag aacaaagcgc
     1441 gcgacgtatg atctgccgtc tgccgtgtga cctcatcgaa ttgatcaatg atgtaggcat
     1501 aagaagtatc cttaaactgc ttgcgatact ctccataaaa gtggacacgt acatcatatt
     1561 ctttatagaa gttgacaaag tctgggtgaa ttgccagcaa agacaggctg gttccgatct
     1621 ggcgcatgta ttcttcgccg cggttgacca ataactcgcc gccaaaagcc ggggcgacga
     1681 tgttatctat gccgtggtca aagaccaatt tataggtatt gatatatcct tgcgtggtca
     1741 actcgatgta ttgtttggca agatcgccgc cagtagatga tggacgctct aacaaaaacc
     1801 agcgacgcgt gccattaaaa ggaaagacac aggtttttac cctatcgccg cggacaaggt
     1861 ttgccaactc ttcggtgggc agggcaagaa aagcttcaag agacatagga gacatttctt
     1921 atgcctcctg cccagcatcc ccggcagaga agagcatatc catggcagag agatactcaa
     1981 accaaccggg gtacatggcg aaatcgtctt tgtggaaagg ctcatcgggg taaagggttt
     2041 tgacgcgcaa gaaatcatat aacgcccgtt gctcacgggt ggatgtttcc acttctgcca
     2101 gagagcgtgg atggttaaag tggcgctccc ccgctgcgac ctgttcggtc atggaacgaa
     2161 tacggtccga tgggtcgcta tcgacttcac attgcgcatc gagaaaacgg cagaggcgtt
     2221 cacattcacg ctgtgggtct ttcgtaagtt ggtcgtacgc catgaatagt ttgcgagggg
     2281 tattggctgt gcattggagc aggttcaaca tgcaaaactg ccagtatgcc aagccagccg
     2341 aaagcggaag ttgctccggg tcgagcccat ccattttggc ggcggaaaaa atcgtctcta
     2401 ctgggtgacg cacgggaatt acatagataa catcacccca tacatctgcc caaaaaggaa
     2461 gcaccaaagg caagcgtggg tctttccaaa cccatgccgc ggcatgattc gcctgcgcct
     2521 cttcatccat ttcagctaag atgctcaagg cacgttctcg caacccggca tcttctgctt
     2581 tttcaagcaa ggtctctaca ggtgtgggaa cgcggctgtt attgtttaga agttcatcgt
     2641 tcaatttctg taaggcgaag tgctccatat agccaaattc gtctttgaaa atatcttctt
     2701 cccgccctgc atacgccccc caacgatgga ccaactctgt gattagtgat gttccacttc
     2761 gataaattcc ggcaacgatg atacgcttca ttgtcatttg tccttttatg tatcaaaaag
     2821 tataaagtta ataactcttc tacggtatga acaaaattaa gaacgctgat ctcttcagga
     2881 attcatatac aacaatcccc catctttagg cacaacccat agactcaaat gcaaaatgtt
     2941 tcggcgaagg gatggctctc agaaaggctt gcccaagcat gggcggagta agaagcggct
     3001 ctttttggat tttgtgcgcc cggtcaattt cccctctgga tatgctatta aatagaaggg
     3061 cttacagtct tgcatcccac gtatattgca ggagtgtcgt tgaagggcgg ttaagcaacg
     3121 ttgtatagaa ccttgattca atgcttgtta cattaattat tttacaggta tatacgactt
     3181 tagaatttaa caaaaatgca agaaacatgt taaattttgg taatgtagaa aaaaggaggt
     3241 ttttcgcggc agggggtgct acttatccta caagttattc ggggttcgct ttatacttga
     3301 ggggcagtca ccacgaaaac aaaggtgaaa catgacttta ttagaccagt tcaacaaatg
     3361 gaaagaaact acgctcaaga agtctttgga taaattcaaa gaacgtaagg gacgttttga
     3421 aaccagcgcc gggatcgaag tgccgcgcct cgcgattccc gatggaaatg aagagcgagg
     3481 ggacctcgcc cctacggtgg cggatgaaaa ttatgtaaac aaacttggtt tccccggaga
     3541 atttccctac acccgcggcg tacagccgac gatgtaccgt tcacgttttt ggacgatgcg
     3601 ccagtatgcg ggcttttcga cggcagaaga atcgaacaag cgttaccgct atttgctcga
     3661 acaaggtcaa acggggctgt cggttgcgtt cgacctcccc actcaaattg ggtatgatgc
     3721 ggatgatccc atcgcgcagg gagaggtcgg taaggtgggc gtgtccattt cgtcgatcaa
     3781 agacatggag cagttattcg atcaaattcc attagataaa gtttcgacat ccatgaccat
     3841 caacgcgccg gcgggagtct tgctggcgat gtatattgcg gttgccaagc gccagggcgc
     3901 ggacatgcgc gaactacgcg gcacgatcca gaacgacatc ctcaaagaat atgtagcccg
     3961 tgggacgtat atcttcccgc ccgccccgtc gatgcgactg atcacggaca ttttccagtt
     4021 ctgctcgaag gaagtgccgt attggaacac gatctcgatc tcgggctatc acatccgcga
     4081 agccggttcg accgccgtgc aggaagtggc gttcaccctc gcgaatggaa tcgcctatgt
     4141 ggaagcagcg ctcaaagccg gactgaacgt ggatgatttc gcgggtcagc tttcgttctt
     4201 cttcaatgcg cacaacaacc tgctcgaaga agttgccaaa ttccgcgcgg cgagacgcat
     4261 gtgggcaaag atcatgcgtg aacgtttcaa ggcacagaag ccgtcttcct ggcagttgcg
     4321 cttccacacg cagaccgctg gctcaactct cactgctcaa cagccggaaa acaatgtagt
     4381 gcgtgtgact ttgcaagctt tgtccgccgt tctcggtggg acacagtctc tgcacaccaa
     4441 tagcatggac gaagccttgt ggctgccgac cgaaaaatcg gtgcgcgttg ccttgcggac
     4501 tcaacagatc atcgcgcacg aatccggcgt agcggactcg attgacccgt tggcaggttc
     4561 gtatct
//
LOCUS       DAOREF010000113         4546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11000_length_4546_cov_4.038967, whole genome shotgun sequence.
ACCESSION   DAOREF010000113 DAOREF010000000
VERSION     DAOREF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11000_length_4546_cov_4.038967"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKI33_03395"
     CDS             <1..692
                     /locus_tag="PKI33_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNM36077.1"
                     /translation="GIFTIMKLPTLLSYLVSLATPLVLIGLALRILLTPLYYTVEYNM
                     PYFPADEYGFTKEDRLKWAKPSVEYLVNSADISYLGDLKFDNGTPIYGKAELSHMHDV
                     KNVVQGALKVWYLSIAVLILLAILAQRNGWMPLYLIGLRRGGWWMIGLAVTLGFIAGA
                     GILLNPDIFWNFFAWFHSLFFEGDSWLFYYSDTLIRLFPIRFWQDAVIAMAVIALGGG
                     LGLAFGLKTTN"
     gene            815..1675
                     /locus_tag="PKI33_03400"
     CDS             815..1675
                     /locus_tag="PKI33_03400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36078.1"
                     /translation="MPLTNDQCLELFGSSQIDLIRMADAHTLNVQAWLSSRSPMAKTF
                     QGMGVRASSTGLKLPLLNLALGANFPDKTASSEIEAEVKQVIDFFAERNVPWYWWMNA
                     EPRPANIATILEKFSCVYDAPPLPAMIAPTKQDASSFPQVDKSIQVWRAASIKDLQMA
                     STIRRRAFQFTEGEALTYFEDMAQDWLSNESSARLFLAGPKKGEAVSIGAIIEAKGIP
                     GIYVMATLPEFHRQGYGKAILSRLLSEAAQCEGKIVALTASSAGAGLYAQFGFRHLFD
                     FNFYSIPDEV"
     gene            1697..2455
                     /gene="fabG"
                     /locus_tag="PKI33_03405"
     CDS             1697..2455
                     /gene="fabG"
                     /locus_tag="PKI33_03405"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNM36079.1"
                     /translation="MSFKLSLENKVAIVTGGSRGIGRAIALEFADRGAAVVVNYNKSP
                     ESAEEVVKKIQEAGGKAAAFQADVSDFKQAEALVKFAIDTFGDLHILVNNAGITRDQL
                     IMMMPEADWDAVINTNLKSTFNCSKAAVKHMMRKRTGRIINMASVAGQMGNPGQTNYS
                     ASKGGQIAFTKALAREVSARNITVNAIAPGFVDTEILDAMTPEALEAALKLVPLGRKG
                     KPEEIAFTATFLASDGAAYITGQVIGVDGGMAMM"
     gene            2469..2816
                     /locus_tag="PKI33_03410"
     CDS             2469..2816
                     /locus_tag="PKI33_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNM36080.1"
                     /translation="MTESTQGKTTVSPEVLTTIASLAALEVPGVSRLAPVSGGVNRLF
                     RRGTSDGVRIEAKDNTVFVDLFLILKDGVNIREVGRNVQANVARSVQEMVGMEVGEVN
                     VHIEDIDFEGDEA"
     gene            2821..3279
                     /gene="nusB"
                     /locus_tag="PKI33_03415"
     CDS             2821..3279
                     /gene="nusB"
                     /locus_tag="PKI33_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNM36081.1"
                     /translation="MKPRTRARALALQVLYETDLSTHLPGDVLKTRLEETTLPQDLAE
                     FARSIIFGILPIRENLDRVIAKYAPEWPLDQIAAIDRNILRMACWEFAISRQTPVKVA
                     INEAVELAKLFGSDSTARFVNGVLGTLVEHEQEIYQVLKNEIPQETNLES"
     gene            3282..3689
                     /locus_tag="PKI33_03420"
     CDS             3282..3689
                     /locus_tag="PKI33_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNM36082.1"
                     /translation="MEILGIGMPELIFVVIIALLILGPKDMQKAGKTIGKFLRDIITS
                     DGWKLFQQTSRDLRTLPNRLIREANEDLDKVNREIKSAASTPDPARSAPANSRPQPQP
                     YSATPPIKPGGENVILPNPQEKNNPESETPNNA"
     gene            3682..>4546
                     /gene="tatC"
                     /locus_tag="PKI33_03425"
     CDS             3682..>4546
                     /gene="tatC"
                     /locus_tag="PKI33_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNM36083.1"
                     /translation="MRKFFKGVWWVITLPFRLVYAIIAFPFRAGYRFFKLLNSEPEER
                     PIADVFADLVTEKEVRDQLWEQIEAFRMHLLRSIIALSILVIAAFWVAEPAMAFLSEP
                     VGGLEKLQAIEVTEEIGVFMRVAMTIGIAIAFPYIAFEFWLFAAPGLKPREKKLGLVG
                     IPLATLLFLLGMAFTFYVLLPTALPFLGSFTSISQFWSAREYFSFVTGLMLWIGGFFE
                     FPLLIYVLTAIGLVNPKFLADQWRLAIVIIAVLAAAITPTVDPVNMGLVMVPMILLYF
                     ISIGLSYLAYAG"
BASE COUNT         1117 a         1107 c         1075 g         1247 t
ORIGIN      
        1 ttggaatttt tacaataatg aaactcccaa ctcttctctc ttatcttgtc tctcttgcca
       61 cgccactggt tctgatcggg ttggcattgc gcattttact aacgccgctt tattacacgg
      121 ttgaatacaa tatgccctac ttccctgccg atgaatacgg cttcacgaag gaagaccgtt
      181 tgaaatgggc aaaaccttcg gtggaatacc tcgtcaacag cgctgatatt tcttatcttg
      241 gcgacctaaa attcgataac ggcaccccta tttatggcaa agctgagctt agtcacatgc
      301 atgatgtcaa gaatgtagtg caaggcgctt tgaaggtttg gtatctctcc attgccgttc
      361 ttattctgct tgccatcctc gcccaacgca acggatggat gcctttatat ctcattggtt
      421 tacgccgcgg cggttggtgg atgatcgggc tggcggtcac gcttggtttc attgctgggg
      481 caggcatttt gctcaacccc gatatcttct ggaatttctt tgcctggttt cactcactat
      541 tctttgaggg cgactcttgg ctcttctatt attcagatac cctcattcgt ctcttcccca
      601 tccgcttctg gcaggatgca gttattgcca tggcagtgat cgccttggga ggcgggttgg
      661 gtttagcatt tgggctaaaa acaacaaact aaaccttctt aaatttatat gttaaccctg
      721 atctgagtga catttctctg aaatcgttgt catcagttcg gggttatttt tttataaatc
      781 taggcaatgc atcatctcgt caaacggaga aaaaatgcct cttaccaatg accaatgtct
      841 tgagctattt gggtcttcgc aaatagacct catccgtatg gcagatgcac atacgttaaa
      901 cgtccaagct tggctttctt cacgcagccc aatggcaaaa acgtttcagg gcatgggagt
      961 cagagcctct tctacagggt taaaactgcc gctcttaaat ctggcgcttg gtgccaactt
     1021 tccagacaag acggcttcat ctgaaataga agcggaagta aaacaagtca tcgacttctt
     1081 cgcggaaaga aatgtccctt ggtattggtg gatgaatgct gagccgcgcc ctgcaaacat
     1141 tgcgacaatt ctagagaaat tcagttgtgt gtacgatgcc ccgccactac cagctatgat
     1201 tgcaccaaca aaacaagatg cttcctcgtt tccacaagta gacaaatcca tacaggtgtg
     1261 gcgtgcggca tctatcaaag atttgcaaat ggcaagtacc attcggcgca gggcattcca
     1321 gtttacagaa ggcgaagcgt tgacttattt tgaagatatg gcacaagatt ggttatctaa
     1381 tgaaagctcg gctcgtttgt tccttgccgg accgaaaaaa ggtgaagcag tttcaattgg
     1441 tgccatcata gaagcaaaag gtatccccgg catttatgtg atggcaactt tgcctgaatt
     1501 tcatcgacaa ggctatggga aagccattct ttcacggctt ctttcagaag cagcccaatg
     1561 tgagggtaaa atagtcgccc taaccgccag ctctgccgga gctggtttgt atgcacaatt
     1621 tgggtttagg cacctatttg atttcaattt ttactcaatt ccagatgaag tataatttct
     1681 ttacaggagc ccagttatgt ccttcaaatt atcattagag aacaaagtag ctatcgttac
     1741 cggtggttca cgcggaattg gacgcgccat cgctctcgaa ttcgctgatc gcggcgcagc
     1801 ggtggtggtg aattacaaca aatcccctga gtcggctgaa gaagtggtga agaaaattca
     1861 agaagcgggt ggaaaagccg ccgcctttca agctgatgtc tccgatttta agcaagccga
     1921 ggcgttggtt aagtttgcca ttgatacttt tggcgacttg catattcttg tcaataatgc
     1981 cggcattaca cgcgaccaac tcattatgat gatgcccgaa gcagactggg atgccgttat
     2041 caacaccaat ctcaaaagca ccttcaattg ctcaaaagct gccgttaagc acatgatgcg
     2101 caaacgcacc gggcgcatca tcaacatggc atcggttgct gggcaaatgg gcaaccccgg
     2161 gcagacgaac tattccgcct ccaaaggtgg tcagatcgcg ttcaccaaag cattggcacg
     2221 cgaagtctct gctcgtaaca ttaccgtcaa cgctatcgcg ccgggatttg tagatactga
     2281 gattctcgat gccatgacac ccgaagccct tgaagctgcg ctcaaacttg tgccgcttgg
     2341 gcgcaaagga aaacccgaag aaattgcctt tactgctacc tttctcgcct ctgacggcgc
     2401 cgcgtacatt accggtcagg tcattggtgt ggacggcggc atggcaatga tgtaaaacag
     2461 gagtaataat gactgaatct acgcaaggaa aaactaccgt ctctcccgaa gtgttgacaa
     2521 ctatcgccag tctcgccgca ttggaagtcc ccggcgttag ccgactggca ccggtcagtg
     2581 gcggcgtgaa tcgactcttt cggcgcggca ccagtgacgg cgtccgcatt gaagcgaagg
     2641 acaacacggt cttcgttgac cttttcctca tcctcaaaga tggagtcaac atccgcgaag
     2701 ttgggcgcaa tgtgcaagcg aatgtagccc gttccgttca ggaaatggtt ggcatggaag
     2761 tgggcgaagt gaatgtccac atcgaagata ttgattttga aggggacgag gcataatcaa
     2821 atgaagcccc gaacccgggc gcgcgcgctc gccctgcaag tattgtacga aactgatctt
     2881 tccacacacc tgcccggtga tgtattgaaa actcgtttag aagaaaccac tctgccgcaa
     2941 gatttagcag agttcgcccg cagcattatt tttggtattt tgcccattcg agaaaacctt
     3001 gaccgcgtga ttgcaaagta tgcacctgag tggccactcg atcaaattgc cgccatcgat
     3061 agaaatattt tgcgtatggc atgttgggaa tttgcaatct cgcgccaaac tccggtaaag
     3121 gttgcgatca atgaagctgt agagttagcc aagttgttcg ggtctgactc gactgcccgc
     3181 tttgtaaatg gtgtgcttgg taccttggtt gagcatgagc aggaaatcta tcaggttctg
     3241 aaaaacgaaa tacctcaaga aaccaatttg gaatcataaa catggaaatc cttggcattg
     3301 gaatgcctga acttattttt gtagtgatta ttgctttgct tattttaggc ccgaaagata
     3361 tgcaaaaagc cggcaagacc atcggaaagt ttttacgaga tatcatcaca tcggatggct
     3421 ggaaactttt tcaacaaacc tcgcgcgact tgcgcacttt gccaaatcgc ttgatccgcg
     3481 aagcaaatga ggatttggat aaggtcaatc gcgagataaa gagcgccgcc tccaccccag
     3541 acccagcccg gtctgccccc gcaaactctc gaccacaacc tcagccatat tctgccacac
     3601 cgcccatcaa gcctggcggt gagaatgtta tccttccaaa cccgcaagag aaaaacaacc
     3661 ccgaaagcga aactccgaat aatgcgtaag ttttttaagg gtgtttggtg ggttatcacc
     3721 cttccatttc gccttgtcta tgccatcata gccttcccct tccgggcggg ttatcgtttt
     3781 ttcaagctgc ttaatagcga acccgaagaa cgtcccattg cggatgtctt tgcggatttg
     3841 gtcacagaaa aagaagtccg cgatcagtta tgggaacaga tcgaagcctt tcgcatgcac
     3901 cttttgcgct cgatcattgc cttatcaatt ttagtgatcg cggcattctg ggtggcagaa
     3961 cctgccatgg catttctctc tgaacctgtt ggcggacttg aaaagctgca agccattgaa
     4021 gtgacagaag agatcggcgt tttcatgcgt gtggcaatga ccattggtat tgcgatcgcc
     4081 ttcccttata ttgcgtttga gttctggctg ttcgccgcac ccggtttgaa gcctcgcgaa
     4141 aagaagttgg ggttggttgg cattccgctt gcgacccttc tttttctgct tggtatggcg
     4201 tttacttttt acgtcttatt accgactgcc ttgccctttt tagggagttt cacgtccatc
     4261 tcgcaatttt ggtctgcacg agaatatttt tcattcgtca ccgggctgat gttatggatt
     4321 ggcggcttct ttgaatttcc gcttcttatt tatgtactta ctgccattgg cttggtcaat
     4381 ccgaaattcc ttgctgacca atggagatta gcaatcgtta ttattgccgt gcttgcggct
     4441 gccatcactc ctaccgtaga cccggtcaat atggggttgg tgatggtccc gatgattttg
     4501 ttatacttta tcagtatcgg cttaagttat ctcgcctacg ccgggc
//
LOCUS       DAOREF010000114         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11117_length_4513_cov_4.400179, whole genome shotgun sequence.
ACCESSION   DAOREF010000114 DAOREF010000000
VERSION     DAOREF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11117_length_4513_cov_4.400179"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKI33_03430"
     CDS             <1..510
                     /locus_tag="PKI33_03430"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNM36084.1"
                     /translation="QGDPVTPLGFAAAFGKIYSDGLLQPTQEPTLYISRRNELSFNRV
                     PGKLQHAISGDRMLLLSGEVVPDLDDTTLQPRTAIGTNRNGRYVILVVVDGRQPFYST
                     GATFKELAELMKAYDAFFAMALDGGGSSTLVVQKKNGGTQILNSPIDNYIPGRERPVA
                     NHIGVSIAP"
     gene            783..2030
                     /locus_tag="PKI33_03435"
     CDS             783..2030
                     /locus_tag="PKI33_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNM36085.1"
                     /translation="MKFKNLFRVAMLFATIISVFGTGAQAYAQGNSTNYLGAQIIIRS
                     TTFWDAVFNGTVNANRYERWSLQLIEPENFTISIITTSGTLTPDIYLLDASENQIASA
                     TGTLSSASLTTNQLAGDYFIQVQPHSGDGTYLMEITRTEQVVEPTASVVLNPAVIPVG
                     GTSTGTLMLSNIPSGGYASAEFTCTYNPALVQISNITDAGLFGSDPAVAVNGPVEGSF
                     VFAVAGSNSQRATSDGAVFTFTVTGLAAGQADIACTVRVSAGGSLSTITSTPASLTIE
                     EVTATLNGTVNATKTVTVTLYKQDTTVEATTTVDTSGNFTITAPAGTYTVVASAPGFL
                     KAQGSPVLTAGATKTMQTISLLAGDIDGNDVIDQFDALTIGMNYNLIEPTAADLNNDG
                     TINVLDLELLAANYRASGASAWQ"
     gene            2093..2755
                     /locus_tag="PKI33_03440"
     CDS             2093..2755
                     /locus_tag="PKI33_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNM36086.1"
                     /translation="MKKFALTLIALICLFVVMPVKAQSAESIWIAASTNSFKTGETVI
                     VTVNGLSATPIQGLTFQIKFDPACLQPVNAASPIAGMNGLSLPQISGFVDASFASTTP
                     QVANGVLAEVRFITLGACQTNLTLESAALVIKNESGFAAPLAGVNLGEKAVALAISAE
                     KGTSADAPLLGTPLALGTEPENSGSSLPAGTTIILAVIGVLLIVGIVVLVRILRGENS
                     RG"
     gene            2795..>4513
                     /locus_tag="PKI33_03445"
     CDS             2795..>4513
                     /locus_tag="PKI33_03445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNM36087.1"
                     /translation="MKAKTFTRLFLILAIFLVAVPTSVFASTAPPVDMFQLPWEQGKA
                     WYPYDGLDNGLKRSSTSPHNYNMGGALDFAPRAKMTVGEDTSNDWVTAAAAGTVYQVG
                     SCYVKIDHGNGWITEYWHLDKIQVTTGTKVSRNQRLAIIHNNKTQQVCIGNEYPGPHL
                     HFVFRPKVLDTRFAGWTVNYNLFTNITTFSKSGQTVGRLQPLMNIPDLQIANRGILDW
                     NINYEGNIDAYRQERWSLMLVDEAKFEINVTPKGIGLVPVIVLLDASGNEITRASGTL
                     STTQPAGFYYVDIRSEAGTGYYNIIATKSESGGSTVTPSPTASETGTFTPTVTATLNG
                     SETPTATGTLTGSETPTATGTLTGSETPTATETPIGFATHTPTSTGVPDTSTPEPSTD
                     TPTPTLTATATFTPTSTPDFEQTAIAATQTSNAATLTAIANEATQTAISASETANAAT
                     LTAIANGATQTAIAATQTFDAQTQTAIAGTLTATADAATQTAIVGTQTAIAGTLTATA
                     DAATQTSIAITQTANVGTQTAIAATQTSDAAMQTAIAGTLTAQPGIQTSIAGTATANA
                     LTMTASA"
BASE COUNT         1123 a         1185 c         1063 g         1142 t
ORIGIN      
        1 cagggagacc cagtcacacc cttggggttt gctgccgcat ttggaaaaat ctactcagat
       61 gggttgctgc aacctacaca agagccaacg ctatacatca gccgtcgtaa cgaattatcg
      121 tttaatcgcg tgccggggaa attgcaacat gctatttcag gagaccgcat gttgcttctt
      181 agtggagagg ttgtgccaga tttggatgat acaactttac agccacgcac agccatcggt
      241 acgaatcgca atgggcgtta tgtgattctc gtagtggtgg atggtcgcca gccgttttat
      301 agtactggtg ccacattcaa agaactggct gagttgatga aagcatatga tgccttcttt
      361 gccatggcat tagatggggg tgggtcttct acattggttg tgcaaaagaa aaatggtgga
      421 acgcaaatat tgaattcacc tattgataac tatattccgg ggcgtgaacg tcctgttgca
      481 aatcatattg gtgtctcgat tgcaccgtaa ttaaaaatag atttttaaat tgatttttca
      541 agacccactt cacatgaagt gggtcttgat tcttaagcag acatgtcgca actattctgc
      601 aatatcgtgg caactcacct gcaacgcata ttgtgcagtt gcaccaatag aatgtaattg
      661 gcttttaagt cttcatcatt gaaccgccac ggttggcagt ttggaggaaa tacgaaacga
      721 tacccttttt tgttatgtag gctttctgcc ttatatcacc ttgattcgaa aaggagaata
      781 cgatgaaatt taagaatttg tttcgcgttg cgatgctgtt cgcaaccatc atcagtgtct
      841 ttggcacggg tgcgcaagcg tacgcgcaag gaaacagcac caattatctt ggcgcacaga
      901 tcattattcg cagcaccacc ttttgggacg cagtcttcaa tgggacggta aatgcgaacc
      961 gctatgagcg ctggtcattg cagttgatcg aacctgagaa cttcaccatc tcgatcatca
     1021 ccacatctgg cacgctcact cccgatatct acttgttaga tgccagcgag aaccagatcg
     1081 cttctgcaac gggtacgctt tcaagcgctt ccctgactac caatcagctt gcaggggatt
     1141 acttcattca agtccagccg cactctgggg atggtactta tttaatggag atcacccgca
     1201 ccgagcaggt ggttgaaccg accgcatccg tggtgttgaa cccggcggtt attcctgttg
     1261 gcggaacctc cacaggtact ttgatgttga gcaatatccc cagtggcgga tatgccagcg
     1321 cagaatttac ttgtacctat aaccccgctt tggttcagat cagcaatatt accgacgctg
     1381 gtttgttcgg ttctgacccc gccgttgctg ttaatggacc cgtcgagggt tccttcgtgt
     1441 ttgcggtagc aggcagcaat agccagcgtg ctacttcaga tggcgctgtc ttcaccttta
     1501 cggtgacagg tcttgcagct gggcaagcgg atattgcttg taccgtccgt gtttcagctg
     1561 gcggttcgtt gagcaccatt acgtccaccc cggcttcgtt gaccatcgaa gaagtgactg
     1621 ccactctcaa tggtacggtc aacgccacca agactgtgac ggtgactctc tataagcagg
     1681 atacgactgt agaagccacc actactgtgg acacaagcgg aaattttacg atcacggctc
     1741 cagcggggac ttacaccgtt gtcgcatcgg ctcccgggtt cctcaaagcc cagggcagcc
     1801 cggtcctcac cgctggtgca accaagacca tgcagacgat ctctcttctc gccggtgata
     1861 tcgatggcaa tgatgtgatc gatcagtttg atgctcttac gattggtatg aactacaacc
     1921 ttattgaacc caccgctgca gacttgaaca atgatggcac catcaatgtt ctcgaccttg
     1981 aattactggc agcgaactac cgcgcttctg gcgcaagtgc ctggcaataa atcccctggc
     2041 aatgcaaagg gtgcaccgat tcatcatcgg tgcaccctcc atggagactt caatgaaaaa
     2101 atttgcactg accctcattg cacttatctg tctttttgtt gttatgcctg taaaggctca
     2161 atctgcagaa tccatttgga ttgctgccag cacgaacagt tttaaaactg gtgaaacagt
     2221 gatcgttacg gtcaatgggc tttctgccac cccgattcaa gggctgacct ttcagatcaa
     2281 gtttgacccg gcatgccttc aacctgtcaa cgctgccagc cctattgctg gcatgaacgg
     2341 gctttccctt ccacagatca gcgggttcgt ggatgcttcc tttgcaagta ccacgcctca
     2401 agttgcgaat ggcgtgttag ctgaagtacg ttttattact cttggtgcct gtcagaccaa
     2461 tttaacgtta gagagtgctg cgctcgttat taagaatgaa tctgggtttg cggctccttt
     2521 ggctggtgta aaccttggag aaaaagccgt ggctctcgcc atcagcgctg agaagggtac
     2581 ctctgccgat gcgcctttgt tgggcactcc actggcgtta ggcaccgaac ctgaaaattc
     2641 cggttcttct ctacctgccg gcaccaccat cattcttgct gtgattggtg tgcttctcat
     2701 cgtcggtatt gttgtgcttg tccgcatttt gcgcggtgaa aattcgcgcg gttaatttta
     2761 tccacagtcc gttttgatag ggcacggtgc cattatgaaa gctaagacat ttaccagact
     2821 atttttgatc ctcgcgatct tccttgttgc tgttccaaca agtgtatttg cgagcacggc
     2881 acctcccgtg gatatgttcc aactgccttg ggaacaaggc aaagcttggt atccatacga
     2941 tggactggat aatggactca agcgttcttc taccagccca cataactaca atatgggcgg
     3001 cgcgttagac tttgctcctc gtgcaaagat gacggtaggg gaagatacct caaatgactg
     3061 ggtcactgcc gcggcagctg gaacggtcta tcaagtcggt tcttgttatg tcaagatcga
     3121 tcatggcaat ggctggatca ccgaatattg gcacttggac aagattcaag tgaccactgg
     3181 caccaaggtg agccgaaatc aacgcttggc gattattcac aataacaaaa cccaacaagt
     3241 ctgtattggg aatgagtatc cggggccaca tttacatttt gtattccgac ccaaagtact
     3301 cgatacgcgc tttgccggtt ggacggtcaa ctacaacttg ttcacgaata tcaccacctt
     3361 ctcaaagagc gggcagaccg taggcaggct tcagcctttg atgaacattc ctgacctgca
     3421 gatcgcgaat cgtggcattc ttgattggaa tatcaattac gaaggcaata tcgatgcata
     3481 tcggcaggaa cgttggtctc ttatgctggt cgatgaagca aagtttgaga ttaacgtcac
     3541 cccgaaagga attgggcttg tcccggtcat tgttttgtta gacgcaagtg gaaacgagat
     3601 tacacgggcg agtggtacat tgagcaccac tcagcctgct ggtttctatt acgtcgatat
     3661 ccgttctgag gcgggcacgg gatactacaa tattattgcc accaaaagcg aatcaggcgg
     3721 ctccaccgtc acgccatcac cgacggcttc agaaacaggg acattcactc caactgtaac
     3781 tgcaaccttg aacggctcag agaccccaac cgcaaccgga accttaaccg gctctgaaac
     3841 tccaactgcg acgggtacgc tcactggttc tgaaacccct acggctactg agaccccaat
     3901 tggtttcgcg actcataccc caacttctac tggtgttcca gatacaagca cgcctgaacc
     3961 cagcacagat actcccacgc caaccctaac ggctactgct acatttacac caacttctac
     4021 acccgatttt gagcagacag cgattgcggc gacgcaaaca tcaaatgcag ccacgttaac
     4081 tgccattgcg aacgaagcaa cccaaaccgc aatttcagcc agcgaaacag caaacgccgc
     4141 aacgctaacg gcaattgcca atggtgccac tcaaactgct attgcagcaa cacaaacttt
     4201 tgatgcccaa actcaaaccg ccattgcagg gacgctcaca gccacagccg atgccgctac
     4261 acaaactgcc attgtcggga ctcaaaccgc gattgccgga acactcacag caactgcgga
     4321 cgcagctacg caaacttcta tcgctataac tcaaactgca aatgtgggca cccagactgc
     4381 tattgccgct acacaaactt ctgatgcagc catgcaaact gccatcgcgg gcacgttaac
     4441 agctcagcct ggaattcaga cttcgatcgc gggcactgcc acagccaatg cactgacaat
     4501 gactgcatct gcg
//
LOCUS       DAOREF010000115         4500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11165_length_4500_cov_4.353881, whole genome shotgun sequence.
ACCESSION   DAOREF010000115 DAOREF010000000
VERSION     DAOREF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11165_length_4500_cov_4.353881"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PKI33_03450"
     CDS             complement(<1..879)
                     /locus_tag="PKI33_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNM36088.1"
                     /translation="MRRPHFPQRHPSNIERRRRRFFPFAFFFFGIFSLFFGGVAAILY
                     WAFTEYSGFRNIWLLVCAAPIALLILGVFTIFTMYMRFGKPLGDIFNAIDSVAEGNLS
                     VRVDESKSDMFSELIKRFNKMVEELERAEQQRRNLTADIAHELRTPLHIIQGNLEGVI
                     DGVYQPSPEHINNTLDETKLLARLVDDLQTLSLAETGQLPLHPTRFLLADLTADLITS
                     FSAQAKTQGIELTAHNADGEKTIEADYDRLNQVLSNLISNALRHTPAGGKISIETESI
                     TGGERSARITIQDTGSG"
     gene            complement(890..1570)
                     /locus_tag="PKI33_03455"
     CDS             complement(890..1570)
                     /locus_tag="PKI33_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36089.1"
                     /translation="MPELILVVDDEPKIVRIARDYLEKNGFRVITAADGHAALATARR
                     EKPDLIVLDLMLPLMDGREVCKTLRRESDVPIIMLTALAEEVDQVTGLEIGADDYITK
                     PFSPRALVARVRALLRRAKGEVKPPSIIRVGGLEIDPDRYSVTFNNEPVKLTPNEFKL
                     LQLLSARPGQTLTREQLLEDMHGIASSFDRSVDSHIKNLRKKLETASGENMIETVYGI
                     GYRFIEIK"
     gene            complement(1671..2147)
                     /locus_tag="PKI33_03460"
     CDS             complement(1671..2147)
                     /locus_tag="PKI33_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36090.1"
                     /translation="MNKGPFIVLRVLGILLILGLIAGAGAFGYKAGLAQGIQQAPEVA
                     AAFEKAAENGQVAMPGAPMMYGYGYPHMYGFGHPHFFNPFGAICFSFLFLFLFFGAMK
                     MLFFRRMRHGWGHHGHHGPWRKDWEGNVPPFFNEWHKRAHGESTEGETPADSEKKE"
     gene            complement(2278..3828)
                     /locus_tag="PKI33_03465"
     CDS             complement(2278..3828)
                     /locus_tag="PKI33_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNM36091.1"
                     /translation="MEITYFEFVKHLIRFNRNVYLDHNATTPVSEHVRNKMDQALKYH
                     YGNPSSFYGSGRKSAGLVEEARGHVANAIHAEPYEVIFTACATESNNAVLKSVSAHFY
                     PQKKKIISTPIEHPSVLNTLAYLETQGVVVEFCPVDNKGRIQLTHLEKMVDNDTFLVC
                     CILANNETGVIQDIQAVTKIAKSHGALVLTDCVQALGKIPIDVHTWDVDYASFSAHKL
                     YGPKGVGALYVKQGSPFTPLLHGGHQEGGLRAGTESTHNIVGFGAACQQVDKLLAHVE
                     EVCALKHDLIQRLKDIKPDCVINSPEVDSECLPNTLSIRFPGVENSGLMGMLDYRGIA
                     VSAGSACSTGEDTPSHVLKAIGLSDQAARETIRISLGHDTSARDIQYTANVIRDYLEG
                     RISFVNMLAPAQLNETILFSDDTFILDVRPSDDRAKFKGIPNAHEVNPLFVENYIKQL
                     PRDKQIIVYCPGGGLSVMISYFLKAKGFKRITNLRGGLDAWRKRRADLYGKYAGQNVT
                     VLEPDAKR"
     gene            complement(3909..>4500)
                     /locus_tag="PKI33_03470"
     CDS             complement(3909..>4500)
                     /locus_tag="PKI33_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNM36092.1"
                     /translation="TAAVTALQGLRDHGKIKAGDKVLINGATGGVGTFAVQIAKAMGA
                     EVTGVTSTKNVELIRSLGADTVIDYSKEDFTQTNKQYDILLDIAGSRTWKEYRKVLKP
                     KANFVIVGGQKGNKFIGPLANALRLRVSALGASQTVAFFIANFSRDDLNLLRELVERG
                     KVKPVVEKVYPLAQAADAMKHLGTGHARGKIVLKVK"
BASE COUNT         1108 a         1082 c         1116 g         1194 t
ORIGIN      
        1 cccagaacct gtatcttgga tggtgatgcg cgcgcttctt tctccgccgg ttatggattc
       61 tgtttcgatt gaaatttttc cacctgcagg cgtgtgtctt aatgcattgg aaatgagatt
      121 ggataatacc tgattaagcc tgtcataatc ggcttcaatc gttttctcac catctgcgtt
      181 atgagcagtc aattcgatgc cttgagtttt cgcttgtgct gaaaaactgg tgatgagatc
      241 cgctgtcaga tcggcaagca ggaaacgggt agggtgaagc ggaagttgtc ccgtttcggc
      301 gagcgagagc gtttgtagat catccaccag ccgtgccagc agtttggtct catccaaagt
      361 gttgttgatg tgttccgggc tgggttgata cacaccgtca atgacgcctt cgaggttgcc
      421 ttgaataatg tgcaaaggcg tgcgcaattc atgcgcaata tcggcggtga gattgcggcg
      481 ctgttgctcg gcgcgttcca gttcttcgac cattttattg aagcgtttga tcaactcgct
      541 gaacatgtca gacttgctct catcaacccg cactgagaga ttcccctctg caacagaatc
      601 gatggcattg aagatatcgc caagtggttt gccgaaacgc atatacatgg tgaagatggt
      661 aaatacgccg aggatcagta aggcaattgg cgctgcacaa accaacagcc aaatattgcg
      721 aaagccagaa tattctgtga atgcccaata taaaatcgcc gctacaccgc caaagaacag
      781 ggaaaagata ccaaagaaga aaaatgcaaa tggaaaaaaa cgccttcgac ggcgctcgat
      841 attggatggg tgtctttggg ggaagtgagg acgacgcatg tgcatggttt tattttattt
      901 caatgaaacg atacccaatt ccatatacgg tttcgatcat attttctccg gaagcggttt
      961 caagcttttt acgtagattc ttgatgtgcg agtctacact gcggtcaaag ctggatgcga
     1021 tgccgtgcat atcttcgagt aattgttcac gggttaaggt ctgccccgga cgcgctgaaa
     1081 gcaactgtaa aagcttgaac tcattaggcg tcagtttgac gggttcattg ttgaatgtga
     1141 ccgagtagcg atcggggtcg atttccaatc ctcccacacg gatgatgcta ggcggtttta
     1201 cttcaccttt tgcacgccgc aacaaggcgc ggactcttgc gaccaaggca cgcggcgaaa
     1261 agggtttggt gatgtaatca tcggcaccga tctccagccc ggtgacttgg tccacttctt
     1321 cggcgagagc ggtgagcatg atgatgggga cgtcgctttc acggcgcaga gttttgcaca
     1381 cttcacgccc gtccatgaga gggagcatca agtccaaaac gatcagatca ggcttttcgc
     1441 ggcgagccgt tgccagcgcg gcatgaccgt cggcagcagt aatcacacga aagccatttt
     1501 tttccaaata gtcgcgcgca attcgcacga tcttgggttc atcatcaaca acgaggatca
     1561 gttcaggcat ggggaaagta taaagggaat gaggaaggat ggatagtgag ggcttaagaa
     1621 aggggaagaa gaaagaggcg tttcttcctc tttcttcttt atttcacacg ttactctttc
     1681 ttctcagagt cggcaggtgt ttcgccttct gtgctttcgc cgtgagcgcg cttgtgccat
     1741 tcattgaaga atggggggac gttgccttcc caatccttgc gccaaggacc gtgatgtccg
     1801 tgatgccccc agccatgtcg catacggcgg aagaagagca tcttcattgc cccaaagaac
     1861 aggaagagga acaagaaaga gaagcagatc gcgccgaatg gattgaagaa gtgcgggtga
     1921 ccaaaaccgt acatatgcgg gtagccatat ccgtacatca tgggtgcgcc gggcatagca
     1981 acctgaccat tctcggcggc tttctcaaag gcggcggcaa cttcaggagc ttgttgaatc
     2041 ccttgtgcca aaccagcctt atacccgaac gcccctgcgc ctgcgatcag cccgaggatg
     2101 agcagaatac cgagaacgcg taaaacaata aacggacctt tattcatttt tacctccaat
     2161 ggtaattgtt tgatttgtcc ttacgcgtct aaatgtatca acccattgtg gagggattat
     2221 ggatggacag gtgagggatt gtggagggaa tgtaggaaaa ttattagcgc ctcttgctta
     2281 acgtttagca tctggctcaa gaactgtcac gttttgcccc gcatattttc catacaagtc
     2341 agcccgtcgt ttgcgccacg catcaagccc gccgcgcaga ttggtgatgc gtttaaaccc
     2401 ttttgctttc aagaaataag agatcatgac cgaaagacct ccgcccgggc aatagacgat
     2461 aatctgctta tcgcggggga gttgctttat atagttttca acgaataacg ggttgacctc
     2521 atgtgcattg ggtattccct tgaactttgc ccggtcatca gagggacgca catcgagaat
     2581 aaacgtgtca tcgctaaaaa ggattgtctc gttcagctgc gcaggagcga gcatattcac
     2641 gaacgaaatc cgaccttcga gataatctcg aattacattt gctgtatatt gaatatctcg
     2701 cgccgaagta tcgtgcccga gactgatgcg aatcgtttcg cgggcagcct ggtcagacaa
     2761 accgattgct ttcaaaacat gcgatggcgt atcttcacca gtactgcacg ccgagccagc
     2821 agagacggcg attccacgat aatccaacat gcccatgagc ccggaatttt ccacaccggg
     2881 gaaacggatg ctgagtgtgt tcggtagaca ttccgaatcc acctcgggcg aattgataac
     2941 gcaatctggt ttgatatctt tgagacgttg aatcaaatca tgtttcaatg cacagacttc
     3001 ttcaacatgc gctagaagtt tgtccacttg ttgacaggcg gcgccaaagc cgacgatatt
     3061 gtgggtgctt tctgtaccag cccgtagccc gccttcttga tgcccaccat gtagcagtgg
     3121 tgtaaatgga ctgccctgct tgacatataa cgctccaact ccttttggac cgtacagctt
     3181 gtgagccgaa aaagaggcgt aatctacatc ccaagtgtgg acgtcaattg ggatctttcc
     3241 caaggcttgc acgcagtctg tcaacaccag cgctccgtgt gatttagcaa tttttgtaac
     3301 cgcctgaata tcttgaatca cgccggtttc attgttggct agaatacagc aaactaaaaa
     3361 ggtgtcgtta tcaaccattt tttcgaggtg agtgagttgg atgcgccctt tgttatccac
     3421 tgggcaaaat tcgacaacca cgccctgtgt ttctaaatat gccaatgtat tcaacacaga
     3481 agggtgttca atcggtgttg aaatgatctt tttcttttga gggtaaaagt gcgctgaaac
     3541 agatttcagt accgcattat ttgattcagt agcgcacgct gtaaaaatca cctcatacgg
     3601 ctcggcatga atggcatttg ccacatgccc tcgggcttct tctactaacc cagctgattt
     3661 tcttccactg ccataaaatg acgaagggtt accataatga tatttcagtg cctgatccat
     3721 tttattgcgt acgtgttcgc tgaccggtgt cgttgcatta tggtcaagat aaacgttgcg
     3781 gttgaagcgg atgaggtgtt ttacgaattc aaagtatgtg atttccattg agactccggg
     3841 ataaagctat gatacggtta ctctcagtat acgttgttcc gaagtaatag ttggtaatgg
     3901 atgccctctt atttcacctt gagcacgatc ttgccgcgtg catgtcccgt ccccaaatgc
     3961 ttcatggcgt cggctgcctg cgccagcgga tagactttct ccacgacggg ttttactttt
     4021 cccctttcaa ctagctctcg taaaagattg aggtcgtcac ggctgaaatt ggcaatgaag
     4081 aaagcaacag tttggctggc acccaatgcc gaaactctca accgaagcgc atttgccagc
     4141 ggaccaataa acttattgcc cttctgccca ccaacaatga caaagtttgc tttgggtttg
     4201 aggacttttc tatattcttt ccacgtccgg cttccggcaa tgtccaagag aatgtcatac
     4261 tgcttattag tttgggtgaa atcttctttg ctgtagtcaa taacagtatc cgcgccgagt
     4321 gagcggatta actcaacgtt tttcgtgctt gtcacaccgg tcacttcggc tcccattgcc
     4381 tttgcaattt gcactgcgaa cgtgcccacg cctcccgtcg cgccgttgat gagaacctta
     4441 tccccagctt tgatcttgcc gtgatctctt aatccttgca gggcggtgac cgccgcagta
//
LOCUS       DAOREF010000116         4497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11179_length_4497_cov_4.712292, whole genome shotgun sequence.
ACCESSION   DAOREF010000116 DAOREF010000000
VERSION     DAOREF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11179_length_4497_cov_4.712292"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKI33_03475"
     CDS             complement(<1..209)
                     /locus_tag="PKI33_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36093.1"
                     /translation="MVAKAKNESRPMTEEADFLQIKSFDHVHFYLGNAKHAMYYWWKA
                     FGFKPVAYSGLETGNRDFASYVLESG"
     gene            complement(306..1229)
                     /locus_tag="PKI33_03480"
     CDS             complement(306..1229)
                     /locus_tag="PKI33_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNM36094.1"
                     /translation="MKTKYFFFLISALIFVSMACRKTANTGVGIQNEYTLNYQNIERS
                     YILYTPKGLDTSKPMPLVFVLHGGGGNAENIIRSSGFNEVADQNGALIVYPNGTGRLN
                     DAILTWNGGTCCGFAQENNIDDVGFIREILKDVESIENVDPKRIYATGMSNGGIMSYR
                     LACEASDIFAAVGSVSGTLNFSPCTPSENVSVIHFHGTDDQHVPYNGGVGSQSLVGVD
                     FASVQDSVNFWVEFNGCNSQPQTNSFEDVQHQAWEGCVGFSSVELYTIIGGKHAWPGS
                     QGPGWVGGDEPDQTISATQLLWEFFASHPKP"
     gene            complement(1244..1990)
                     /locus_tag="PKI33_03485"
     CDS             complement(1244..1990)
                     /locus_tag="PKI33_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNM36095.1"
                     /translation="MNLQSFFSVNYTDTPLQIFGSKHLIVVAIFVLVWLSFVYFRKTW
                     NDKQKNTIRIALAIFLAVNEIGLHIWSAYWGIWNIQTMLPLHMCSVMVWVTTYTALTD
                     NRTLYDFTYFLGIGGALQAFLTPVDVAMYDLPHYRSLQSLIAHGLLITIPIFMTVVEG
                     YRPTFASFKRIFIWTNIYMVIIFFLNRVIGSNYLFIAQKPPSPTLMDMLSPWPWYIPQ
                     LEVVAFIIFFILYIPFLIKDQRAKQSVRQA"
     gene            complement(1994..2488)
                     /locus_tag="PKI33_03490"
     CDS             complement(1994..2488)
                     /locus_tag="PKI33_03490"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNM36096.1"
                     /translation="MIGKLDPEVLRANETKMNKRGKIIGPVMMVGSVLLSVILGIIAY
                     GSTIQRINWSQTSGTVTRSESSSDIIRNEEKYYYEYEYVVDGVTYTGKDSDTGRTVSN
                     PPSKGTSITVYYNPANPEKSLTDTNKSDTISDFAVWMFCCGPIFFLFGAVILFMSTRK
                     SQIA"
     gene            complement(2537..2995)
                     /locus_tag="PKI33_03495"
     CDS             complement(2537..2995)
                     /locus_tag="PKI33_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HNM36097.1"
                     /translation="MKDIYNFLYYSDKLSSSGMPTETQVKSIAEAGVQLVINLAPHDV
                     PHAIPEEEKVVHSFGMEYINIPVNWGTPTRDGLHVFMDNMDANAGKKIHVHCEANFRA
                     SAFVAIYRILRLGWKEEEAFGIMHKIWDEDAYPVWKMFIENAIQRSADGS"
     gene            complement(2992..3468)
                     /locus_tag="PKI33_03500"
     CDS             complement(2992..3468)
                     /locus_tag="PKI33_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNM36098.1"
                     /translation="MSTLSPSAQKIQNLLNELGFNYTVIEHVESTRTAQEAADQAGCE
                     LGQIVKSLIFRGKTSGKPILVLTSGPNRVNEKQISEYAGEPIGKADADFVRAVTGYAI
                     GGVPPLGHVEPMETYLDEDFSKYATVWAAAGTPKAIFELNTEDLQKMTGGKVVKVV"
     gene            complement(3490..3900)
                     /locus_tag="PKI33_03505"
     CDS             complement(3490..3900)
                     /locus_tag="PKI33_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36099.1"
                     /translation="MTTPEHQFDFWLGDWNAAWEGGEGTNHIEMAFDGKVVQENFKAQ
                     DLIGMSVSCYDPERTLWCQTWVDNNGSYLDFTGGFENGKMILSRDAIVKGEPCKQRMV
                     WYNITPNEFDWNWERSDDGGKTWQVKWQIKYTRQ"
     gene            3986..>4497
                     /locus_tag="PKI33_03510"
     CDS             3986..>4497
                     /locus_tag="PKI33_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNM36100.1"
                     /translation="MTTQEEIYKTEGDKYEALIAREDYQGNILKTLREITSLEKRLVL
                     DLGAGTGRLACMLAPHVSHVHAFDISEEMLRVCKHKFSASGLSNWQVDLADHRKLPVP
                     DQSADLVVSGWSLSYLAVWNPDTWREELESWMAEMKRVLKPGSFIVLYESLGTGNEAP
                     IKLDHLKDFYP"
BASE COUNT         1203 a         1024 c         1028 g         1242 t
ORIGIN      
        1 cccgattcaa gtacatacga ggcgaagtcg cggttgccag tttctaaccc tgaatacgca
       61 acaggtttga agccaaatgc cttccaccaa taatacatgg catgtttggc gttgcccaaa
      121 taaaagtgga cgtggtcgaa agattttatt tggaggaagt ctgcttcctc ggtcatgggg
      181 cgtgattcat tcttggcttt cgctaccatt ttctctcctt taggttggtt ttcccatgga
      241 tatgggatga ataatgtctt taggattcta cgataaaaga cctatggatg caattaaaaa
      301 ataaattaag gttttgggtg agatgcgaaa aattcccaca acaattgagt ggcagaaatg
      361 gtttgatccg gttcatcgcc gccgacccaa ccgggacctt ggctgccggg ccaggcgtgt
      421 tttcctccaa tgatagtgta taactccacg gaggaaaaac ctacgcatcc ttcccacgcc
      481 tgatgctgaa catcctcaaa agaattggtt tgtggctgtg agttacagcc attaaattca
      541 acccaaaaat tgacagagtc ttgaaccgag gcgaaatcta cccctacgag cgattgagac
      601 cctacaccgc cgttataagg gacgtgctgg tcatctgtgc cgtgaaaatg aatgacggaa
      661 acattttcgc tcggcgtgca aggagaaaaa ttaagcgtgc cagagacaga cccaacggct
      721 gcaaaaatat ctgaagcttc acatgcaagc cgatatgaca tgatgccccc gttcgacatc
      781 cctgtggcgt agattcgttt cgggtcaaca ttttcgatgg actccacatc cttcaggatt
      841 tcacgaatga aaccaacatc atcaatgttg ttttcctgag caaagccgca acaagtgccg
      901 ccgttccagg taaggattgc atcattgagt cgacccgtcc catttgggta aacgatcaat
      961 gctccgttct ggtcagcaac ctcattaaaa ccactggagc ggataatatt ttctgcattc
     1021 ccgcctccgc cgtgtaatac aaataccagc ggcataggtt tagatgtgtc gagcccttta
     1081 ggagtataaa ggatgtaact ccgctcgatg ttttggtagt ttaaagtgta ttcattctga
     1141 atacctacac ctgtatttgc cgtttttcta catgccatgc taacaaatat cagtgcagag
     1201 attagaaaaa agaaatactt ggttttcatg ggcaaaacta gtattacgcc tgacgaactg
     1261 actgctttgc tctttgatct ttaatgagga acgggatgta cagaatgaag aaaataatga
     1321 acgccaccac ctcaagttgt ggaatatacc agggccaggg agagagcatg tccatgaggg
     1381 tgggggaagg cggcttttga gcgataaaca ggtaattgct gccaatgacg cgatttagga
     1441 agaaaatgat gaccatgtag atattcgtcc agataaagat gcgcttgaaa gatgcaaatg
     1501 ttggacggta gccttcaacc acggtcataa aaatcgggat ggtgattagc aatccgtggg
     1561 caatcaatga ttgcaagctg cgataatgtg gcaggtcgta catggcgaca tccaccgggg
     1621 tgagaaaagc ctgtagtgca ccgccaatgc ctaaaaagta agtgaagtcg tagagagttc
     1681 ggttatctgt gagagcagta taggtagtga cccaaaccat aacactacac atatgcagag
     1741 gaagcatggt ttggatattc caaatgcccc aatacgccga ccaaatgtga agaccaatct
     1801 cattgactgc taaaaaaata gccagtgcga tgcgtatcgt gtttttctgt ttatcattcc
     1861 aagtcttgcg gaagtagaca aaggaaagcc agaccagaac aaaaatagca acaacgatca
     1921 aatgcttaga gccaaatatc tggagcggtg tatctgtata gttgacagag aaaaaacttt
     1981 gtaggttcat agtttatgca atttgtgatt tgcgtgtaga catgaatagg ataactgctc
     2041 caaatagaaa gaaaatggga ccgcagcaaa acatccatac ggcaaagtca ctgatagtgt
     2101 cgcttttgtt ggtatcggtt aaagattttt cagggttagc agggttgtaa tacaccgtaa
     2161 tcgatgtccc ttttgagggc gggttgctga ctgtacgccc tgtatcggag tctttcccag
     2221 tgtaagtgac gccatccact acatattcat attcgtaata atatttttct tcatttctaa
     2281 taatatcgct gctagactcg gatcgagtaa ctgtcccgct agtttgcgac caattgattc
     2341 tttgtatcgt gctaccatag gcaatgatcc ccaagattac actcaaaagt acagagccaa
     2401 ccatcatcac tggtccgata atcttgccgc gcttattcat ttttgtttca ttggcgcgta
     2461 aaacttctgg gtcgagtttt ccgatcatag cttctcccta tcaattggat ttttggatgt
     2521 aataatattc tacccgctaa gaaccgtctg cgcttcgttg gatggcattc tcaatgaaca
     2581 ttttccagac cgggtatgca tcttcatccc aaatcttatg cataattcca aacgcctctt
     2641 cttccttcca gcctaatctc aagatgcggt agattgctac aaatgcgctg gctctaaaat
     2701 tcgcctcaca atggacatgg atctttttgc cagcattggc atccatgttg tccatgaata
     2761 catgtagtcc atcacgtgtt ggtgtacccc aattgaccgg aatgttgatg tattccatgc
     2821 caaaagaatg aaccactttc tcttcttctg gaattgcatg tggaacgtcg tgtggagcaa
     2881 gattaatgac caattgcaca cccgcctcag ctatcgactt tacctgagtc tctgttggca
     2941 taccactgct ggagagctta tcgctgtagt atagaaagtt ataaatgtct ttcatactac
     3001 tttgaccact ttcccgccag tcattttttg caggtcttcg gtgttcagtt caaagatggc
     3061 tttgggtgtt cccgctgctg cccatactgt ggcgtatttt gaaaaatctt cgtcgagata
     3121 ggtttccatt ggttctacat gacccaaggg aggcacgcca ccaatggcat accccgtcac
     3181 agcgcggaca aaatccgcat ctgctttgcc gatgggttcg cctgcatatt cgctgatctg
     3241 tttctcgttt acacggtttg gacctgaagt aagcactaga atcggcttgc cgctggtctt
     3301 tccgcgaaag atcagtgact tgacgatctg acccaactcg cagccagcct ggtctgcggc
     3361 ttcttgcgca gtgcgtgttg actcaacgtg ctcgatcact gtatagttga aaccaagctc
     3421 gtttaataga ttttgaattt tttgggcaga aggagaaagt gtggacatct gcccattata
     3481 caaaacaacc tattgacgcg tgtatttaat ttgccatttc acttgccagg ttttcccgcc
     3541 atcgtcagag cgctcccagt tccagtcaaa ttcattgggt gtgatgttgt accaaaccat
     3601 gcgttgtttg catggctcgc ctttcacgat cgcgtcccgg ctgagaatca tctttccgtt
     3661 ttcgaagccg ccggtaaagt cgagataaga gccgttgtta tccacccagg tttggcacca
     3721 caatgttcgt tcagggtcat agcatgaaac gctcatgcca atcaggtctt gggctttgaa
     3781 attttcctga actacttttc cgtcgaatgc catctctata tgatttgtcc cttcgccgcc
     3841 ttcccatgcg gcgttccaat cgccaagcca aaaatcaaat tgatgttctg gggttgtcat
     3901 tttctctcct tgaattttga aaaagcatag cataagaaga aatgagaaaa accctgccac
     3961 agaactattt atgtataata tttggatgac aacacaagaa gaaatttata agactgaagg
     4021 ggataagtac gaagccctga ttgcacgcga ggattatcaa gggaacattc tcaagaccct
     4081 gcgcgaaatc acctctctcg aaaaacgcct cgttctggac ctcggagcgg gtaccggacg
     4141 cctcgcctgt atgcttgccc cgcatgtgag tcacgtccat gcctttgata tctctgagga
     4201 aatgctgcgg gtttgtaagc ataagttttc tgccagcgga ctttctaact ggcaagtaga
     4261 ccttgctgac caccgcaaat tgcctgtacc agatcaatct gcggacttgg ttgtatctgg
     4321 ctggagttta agttatctcg cggtctggaa tccggatacg tggcgtgaag agctggaaag
     4381 ctggatggcg gaaatgaagc gcgtgcttaa gccgggcagt tttatcgtcc tgtatgaatc
     4441 gctgggaact gggaacgagg ctccaatcaa gttggatcac ctcaaagatt tttatcc
//
LOCUS       DAOREF010000117         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11187_length_4496_cov_3.554830, whole genome shotgun sequence.
ACCESSION   DAOREF010000117 DAOREF010000000
VERSION     DAOREF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11187_length_4496_cov_3.554830"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1310
                     /gene="dinB"
                     /locus_tag="PKI33_03515"
     CDS             150..1310
                     /gene="dinB"
                     /locus_tag="PKI33_03515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNM36101.1"
                     /translation="MARTILHLDLDAFYCSVEETQNPQLRGKPFAVGGRPDERGVVAS
                     CSYAARALGIRSAMPMSRAVRLCRDLIIVPGRHHLYGEYSAKVMEKLHNLTPLVEQIS
                     IDEAFLDISDIQADKTRLALDLQLSIRNELNLPSSIGIASNKLVAKIATEVGKKSSKK
                     KNEPPFGFTVVPVGDEAKFLDPLPADMLWGVGPKTNARLAELGIHTIGDIARWPEKEL
                     VSLFGENGRDLWQHAHGMDNRPVSTESETKSISQETTFNVDISDQKTLEKTLREQAHE
                     VARQLRKNDLAGKTVKIKIRWPDFTTLTRQTTLPTSTDNEDDIYRAAVKLMDSVRKPN
                     QAVRLLGVGVSGIGAPVRQLSLWDVGSEKSRKLQEVVDELQEKYGKDVIWKG"
     gene            1391..2353
                     /locus_tag="PKI33_03520"
     CDS             1391..2353
                     /locus_tag="PKI33_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNM36102.1"
                     /translation="MTNTATRLITLIFLLQNQPNQKALELAEKLGVSLRTVHRYFEML
                     DEMGVPVYSERGPYGGFSLVRGYKMPPLVFTLEEAVAVVLGTGLVQELWGDLYRESAN
                     GALAKLENLLPEEQTREVAWARSSLAVTGLHRANLKMQTPALEKLRRAIRELRSVNML
                     YQTSQVPHPAERGLDPYALVHRWGWWYVIGLCHVHNEVRTFRVDRISEVTLSENIFVK
                     STGFDLQEYLNMEMQSQPQITARLKFDVASANIVSGNQSYWQSVEPQADGSVEVTFLS
                     PTLEWAASTVLMYGPAVEVLEPPALRSMVAEWLEVTIRKYTEKE"
     gene            2359..3006
                     /locus_tag="PKI33_03525"
     CDS             2359..3006
                     /locus_tag="PKI33_03525"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNM36103.1"
                     /translation="MKILDLKKQYKNLYQPSAKRIELVQVPNLQFAMVDGAIEKGSEP
                     GKSPSFAQATETLYSLSYTLKFMFKKRKANAIDYPVMALEGLWWVEDGFFDITVKDNW
                     FYTLMIMQPDIITAEIFEEAREQVRKKKGDSEMLNKARLTHFEEGLCVQTMHIGPYAT
                     EPATIDRMKDFMAENNLKDAVGPIGGKHHEIYLSDPRKSAPDKMKTVLRHPVIKV"
     gene            3050..3409
                     /locus_tag="PKI33_03530"
     CDS             3050..3409
                     /locus_tag="PKI33_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36104.1"
                     /translation="MNCFYHSAQPAIGLCKYCQRGLCSECAAVVDDVLACKKRHEDEV
                     HKLEVLSARNLFQSERVGSVYMKNFTFYGLVGLAFVGFGWWQLKWLGLQAVLFIVLGA
                     FLLYASVANYLEGKKYK"
     gene            3409..4164
                     /locus_tag="PKI33_03535"
     CDS             3409..4164
                     /locus_tag="PKI33_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNM36105.1"
                     /translation="MGTWICHLRIAENLLPHLPEIDPITFTFGNLAPDSGIPNEDWTE
                     FDPPKEVTHFLNKGEGEGYIRDLVYYREYLANLEVDKNLQKYSFRLGYFTHLICDALW
                     YYYLDDTMQAGYRELIEKDASKAWGLFKKDWYSLDQQYNQIHKEGLFWKVIFLNPNPP
                     SFLPFVRENALHQQYDYIRKFYSEYDPYWFEERTFPYLNAATMDRLVENMTQATLFIL
                     ENIKSAKHQDLQSSFQLLPQAMQRPYEMPLGDV"
     gene            4224..4415
                     /locus_tag="PKI33_03540"
     CDS             4224..4415
                     /locus_tag="PKI33_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNM36106.1"
                     /translation="MSDIKVLTRSKSNRMIAGVCAGLGDYLNIDPTVVRLLFVLGFFT
                     FHGAMLFVYLVMALVTPEQ"
BASE COUNT         1284 a         1069 c         1017 g         1126 t
ORIGIN      
        1 ttaagtgaaa agttcccttt tgactatacg ctaccatagt ataacttttc tttaataatt
       61 gttcaataat gaaacagaac aaaatcatag tcaatttttc ccatttttct gtgctcttag
      121 ggcaaaatcg actgcactat aataccaaca tggctcgcac gatccttcac cttgacctcg
      181 atgcatttta ttgctccgtg gaagagaccc aaaacccgca actgcgcggc aaaccgtttg
      241 ccgttggtgg cagacctgat gaacgtggtg tggtcgcatc atgttcatat gcggctcgcg
      301 cgcttgggat tcggagtgcc atgcccatgt cgcgagcggt gcgcctatgt cgtgatttga
      361 tcatcgtacc cgggcggcac catttgtatg gagaatactc tgccaaggtg atggaaaagc
      421 tgcataacct gactccactc gtggagcaaa tttccatcga cgaagccttc ctcgacatct
      481 cagatattca agcggataaa acccgcctcg cgctcgacct gcaactgtcc atacgaaatg
      541 agctgaatct accctcgagc attgggatcg cgtccaacaa actcgtggca aaaatagcca
      601 ctgaggttgg taagaaatca agcaagaaaa agaatgagcc accattcggc ttcaccgttg
      661 ttcctgttgg agatgaagcc aaatttctag acccactccc cgctgatatg ctttggggtg
      721 tgggaccaaa aaccaatgca aggttagccg agcttggcat tcataccatt ggggatattg
      781 cccgctggcc cgaaaaggaa ctcgttagcc tcttcggcga aaatggacgc gacctctggc
      841 aacatgcaca cggcatggat aatcgtccag tcagtaccga atcagagacc aagtctatta
      901 gccaggagac cacgttcaac gtagatatca gcgaccaaaa gaccttagaa aaaacattgc
      961 gcgaacaagc tcacgaagtg gctcggcaac ttcgcaaaaa tgacctagcc ggaaaaacag
     1021 tcaaaataaa aatccgctgg cccgacttca ccaccctaac aagacaaacc accttaccca
     1081 cttctacaga caacgaagac gatatctacc gcgccgccgt caagttgatg gactctgtcc
     1141 gcaagccaaa tcaagccgtg cggttgttag gagtcggcgt cagcggaatc ggagctccgg
     1201 ttcgtcaact cagcttatgg gacgtaggaa gcgaaaagtc ccgcaagtta caagaagtag
     1261 tggatgaact ccaagagaag tatggcaaag acgtcatatg gaaaggctag tgctcaaacc
     1321 caaaaattgc taacgggtat atgtcataat ttgacatata cccgttttat attattgaag
     1381 gatgtgacac atgaccaaca ctgccacccg cctcattacg ctcatttttc tattacaaaa
     1441 ccagccaaac caaaaagctt tggaattggc tgagaaactt ggcgtctcat tgcggacagt
     1501 tcaccgctat tttgagatgc tggatgaaat gggtgttccg gtctattctg aacgcggacc
     1561 gtatggtggc ttctcgctcg tgcgtggata caaaatgccg ccgctggtct ttacgttgga
     1621 agaagccgtt gctgtagtat tgggcacagg cttggtgcaa gaattgtggg gagacttata
     1681 tcgcgagtct gcaaatggag ctttggcgaa actggaaaat cttttgccag aagaacagac
     1741 gcgtgaagtt gcctgggctc gcagttcact tgccgttaca ggattgcacc gtgcgaatct
     1801 aaaaatgcaa acacctgcgc tagaaaaatt acgccgtgcc attcgcgagc ttcgcagtgt
     1861 gaatatgctc tatcagacca gtcaggttcc gcaccctgca gaacgcgggc ttgacccgta
     1921 tgcacttgtc catcgttggg gctggtggta tgtaatcggg ctctgccatg ttcacaatga
     1981 agtccgcaca tttcgtgtgg atcgcatttc cgaagttacg ctgtcagaaa atatctttgt
     2041 caaatcaaca ggttttgatt tgcaagaata tttaaacatg gaaatgcaat ctcagccgca
     2101 gatcacagcg cgtttgaaat ttgatgtagc ttcagcgaac atcgtttcag ggaatcaatc
     2161 ctattggcag agtgttgaac cgcaagcaga tggctctgta gaagttactt tcctatctcc
     2221 cacgttggaa tgggcagcca gcaccgtgtt aatgtatggt cccgccgtgg aggtgctgga
     2281 accgccagcg ttgaggtcga tggtggctga atggcttgaa gtcacaatcc gaaaatatac
     2341 cgaaaaggaa tgaagactat gaaaatcttg gacctaaaaa aacaatataa aaatctatat
     2401 cagccctcgg caaaaaggat cgaattggta caagtgccga acctgcaatt tgccatggtc
     2461 gacggtgcca tcgaaaaagg ctccgaaccg ggcaaatcgc catcatttgc tcaagctacc
     2521 gagaccttgt atagcctgtc gtatacactc aaattcatgt tcaaaaaacg caaggcgaat
     2581 gccatcgatt atcccgtcat ggcattggaa ggattatggt gggtggaaga tggtttcttc
     2641 gatatcaccg tcaaagataa ttggttctac acattgatga tcatgcagcc agatattatt
     2701 acagcagaaa tttttgagga agcacgtgaa caagtccgca agaagaaagg tgacagtgaa
     2761 atgctcaaca aagcacgact gacacacttt gaagaaggct tgtgcgtaca aaccatgcat
     2821 atcggaccgt acgccaccga acccgctaca attgaccgta tgaaagactt catggcagaa
     2881 aacaatttga aggacgccgt cggaccgatt ggcggaaaac atcacgaaat ctatctcagc
     2941 gatccacgca agtccgcgcc tgataaaatg aagacagtat tgaggcaccc agtcatcaaa
     3001 gtttaaaggt tggaaagtta tcacatttga acgttcaaac gaggcatata tgaactgttt
     3061 ttaccactcc gctcaacccg ctattggact ttgcaaatac tgccagcgcg gactctgctc
     3121 cgaatgtgcc gccgttgttg atgatgtact cgcctgtaaa aaacggcatg aagatgaagt
     3181 tcacaaactg gaagttctga gcgctcgaaa cctgtttcaa tctgaacgag tcggttctgt
     3241 gtatatgaaa aacttcacct tctatggatt ggtaggattg gcatttgttg gttttggctg
     3301 gtggcaactc aagtggcttg ggttgcaagc tgtcctattt attgtgctgg gagcattcct
     3361 gctctatgct tccgttgcca attatttgga agggaaaaag tataagtaat gggaacctgg
     3421 atttgccacc ttcgcattgc tgaaaacctc ttaccccatc taccagaaat tgacccaatc
     3481 actttcactt tcggcaatct tgccccagac tcaggcattc ccaacgagga ttggactgaa
     3541 ttcgacccgc cgaaagaagt aactcatttt ctcaacaaag gtgaaggtga gggctatatc
     3601 cgcgatcttg tgtattatcg tgaataccta gccaatcttg aagtggacaa aaacctgcaa
     3661 aaatatagtt ttcggctggg ctatttcact caccttattt gcgatgcact ctggtattac
     3721 tatctcgatg acaccatgca agcggggtat cgtgaactga tagaaaaaga tgcctcaaaa
     3781 gcatggggct tgttcaaaaa agattggtac agcctcgacc agcagtacaa ccagatacac
     3841 aaagaaggtc tgttctggaa ggttattttc ctcaacccta acccaccttc ttttttgcct
     3901 tttgtacgag aaaatgcctt gcaccaacaa tatgactata tccgcaagtt ttacagtgaa
     3961 tatgacccgt attggtttga agaaagaacc ttcccatatc tcaacgctgc taccatggat
     4021 cgccttgtag aaaatatgac acaagctaca ttattcattt tagagaacat caaaagcgca
     4081 aaacatcaag atctacaaag ttcatttcag ttattaccac aagccatgca gcgtccatat
     4141 gaaatgccct taggcgatgt ttaatctgca actccttttg cttctttgcg taatacaagt
     4201 gtactcaaac gaaaggagca aaaatgagcg atatcaaagt tctcacgcgg agcaaatcca
     4261 accgcatgat tgcaggtgta tgtgctggtc taggcgacta tctcaatatc gaccctaccg
     4321 tcgtccgcct gttatttgta cttggtttct tcaccttcca tggtgccatg ttatttgtgt
     4381 acctcgtcat ggcgctggta acccccgaac aataatacag taggtcacgc ttcaggcgtg
     4441 acctacatct ttaacgagcc aacgctaaaa agacatcctc cagagttggc tctcct
//
LOCUS       DAOREF010000118         4495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11190_length_4495_cov_4.804955, whole genome shotgun sequence.
ACCESSION   DAOREF010000118 DAOREF010000000
VERSION     DAOREF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11190_length_4495_cov_4.804955"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1349
                     /locus_tag="PKI33_03545"
     CDS             87..1349
                     /locus_tag="PKI33_03545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNM36107.1"
                     /translation="MNSNHGKHGSVFTRAWDIISRDDTIPPEEKKAILRLIQVIVEAR
                     EQASDKRVGDDEEKSITHEVISKHSLMMTVKHQADELDALRRISLNLTSNLDLQKVLD
                     ILVTESMGLIKNANNVTIFLYERGELEFGAALDDKGERNIPVSKPRKNGLTYSVATSG
                     QQIIVEDMKSHPLYQNTSLNLTGSIIGVPLKFKDEVVGVMNLSKKVVGKFTRSELRLI
                     NLLADQAAVAIFNASMYKRLAQMANTDSVTGLPNRRALDERLQEEWRLAEQTKFPFAV
                     VMMDLDGFKAVNDTFGHNIGDDLLYSLFNFLAQRMRSSDFLARYGGDELTLVMRNTDA
                     EAAQSVMKKILELMQDYHFPFPKGKDMKLGITAGIAIYPTHANTPSDLIRAADSALYH
                     AKKYARGEYVVAKAITGRLNPVQVKDGS"
     gene            1421..2344
                     /locus_tag="PKI33_03550"
     CDS             1421..2344
                     /locus_tag="PKI33_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNM36108.1"
                     /translation="MPRKVKLILNPMADMGRAWKTANALRPIAQEFQGELTWSGTVYP
                     THAIELARQAAEEGYDMVIAMGGDGTVHEVMSGLMQVPAEKRPVMGVVPIGSGNDFAY
                     SIGIHQKPDHALAHALKAENIKPVDIGLMTDEHGRKEYFDNTLGIGFDAVVTIRSHKL
                     PIVKGFLMYLTAVIQTILLNHNPAKLNIETDHQSWSASALMLTLCNGPREGGGFMLSP
                     KSKNDDGTMEFLIVNKVSRAMMFRLVPEFMSGTHMRFKQIQMGEFKRFSLTSDSPLYI
                     HADGEIFTSFGSNLKKVAFEILPHALKVVHG"
     gene            complement(2381..3175)
                     /locus_tag="PKI33_03555"
     CDS             complement(2381..3175)
                     /locus_tag="PKI33_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36109.1"
                     /translation="MNIEINDLRFTYPTGLEALKGINLTIQSGEQVAIVGQNGAGKTT
                     LVRHFNGLLRPSSGTVLIGDWETSKYSTAKLASRVGYVFQNPDEQLFSRDVLTEVAFG
                     PRNLGYAKEKVDDLVKRALSLTELSDKTETNPYDLSPTWRKMVALASIIAMDTPIVIF
                     DEPTTGQDAVNVGRIAHVIAELKREGKTVITITHDIDFCAENFDRVIALSKGQILLDG
                     IARDVLGQEEILSQTYVDPPQLTRLGKALGLKETVTKRDEFLKAIE"
     gene            complement(3201..4046)
                     /locus_tag="PKI33_03560"
     CDS             complement(3201..4046)
                     /locus_tag="PKI33_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36110.1"
                     /translation="MAFVNIQNLTYKYPLVKEPVLRNITAQIQEGEFVSIVGPNGAGK
                     STFCYALAGFAPHFFKGEFSGEIEVSGKASSKSTLDEWVLNVGLAFQNPFNQISGAKY
                     TVFEEIAFGLENIGVPREEIKSRVQDAMSLTGISELADRSPYSLSGGQQQRVALTSIL
                     VMRPKLLVLDEPTSQMDPIGTREVFGVVRKMAEEGMTVVMVEHKVEWIAEFADRVIAL
                     KEGQILLEGKPNEVLTSDTLAESGFGISRYTSTAREAKKRGLWKKETLPVTLDEAVEG
                     FKRHA"
     gene            complement(4034..>4495)
                     /locus_tag="PKI33_03565"
     CDS             complement(4034..>4495)
                     /locus_tag="PKI33_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNM36111.1"
                     /translation="VMFAFRNAMRIVVMVSGFTIFLLTTHPSELMSDLTRRGLPGQFA
                     YVIISTLQILPQMQAKAQTIISAQRSRGLDTESSFLKRAGSLIPLIGPLVFGSLVEVE
                     ERAIAIEARGFTSKQVKTSLHEIPDTASEKTLRWGLLALIVISIGLNLWLS"
BASE COUNT         1168 a          949 c         1179 g         1199 t
ORIGIN      
        1 tcgaaataga atatgtatat gaaagtgtat aatagtacaa aggatgtctc gacagataat
       61 gctttttggg cataattcta aaaaacatga atagcaatca tggcaagcac gggtcggtct
      121 ttacgcgagc gtgggatatt atctctcgtg atgatacgat tccgccagaa gagaaaaaag
      181 ccatccttag gctcattcag gttattgttg aggcacgtga gcaggcttct gataaaagag
      241 taggggatga tgaagagaaa tccattaccc atgaagtgat ctccaaacac tcgctgatga
      301 tgaccgtcaa gcatcaggca gatgaactgg atgcgttaag gcgcatcagc ctgaatttga
      361 cctcgaactt ggatttgcaa aaagtgttgg atatcctcgt gacagagtcg atggggttga
      421 tcaagaatgc aaacaatgtg accatcttct tatatgaacg cggcgaactg gaatttgggg
      481 ctgcattgga tgacaaaggg gaaagaaata tcccggtttc aaaaccacga aagaatgggt
      541 tgacgtattc tgtcgccacc tcgggacagc agattattgt tgaagatatg aaatctcatc
      601 cgctttacca gaacacctca ttgaacttaa cagggtcaat tattggtgtg ccgcttaaat
      661 ttaaagatga ggttgtgggg gtgatgaacc tttcgaaaaa ggtagtggga aaatttaccc
      721 gctctgaatt gcgtcttatt aacttattgg cagatcaggc ggcggtagcc atcttcaacg
      781 ctagcatgta taaacgcttg gcacaaatgg cgaatacgga tagtgtgacg ggattaccga
      841 atcgccgtgc cttggatgaa cgtttgcaag aagaatggcg gcttgccgaa caaaccaagt
      901 ttccctttgc agttgttatg atggacttgg atggtttcaa agcggtaaat gacacctttg
      961 gtcataatat tggggatgat ctgttgtatt ctttgttcaa ttttctagca cagcgaatgc
     1021 gtagttcaga tttccttgca cgttatggcg gggacgagtt aactttggtc atgcggaata
     1081 cagatgccga agcggctcag tctgtgatga aaaagattct cgaactcatg caggattatc
     1141 acttcccatt tccaaaaggg aaggatatga aattgggcat tacagcaggg attgcgattt
     1201 acccgaccca tgccaatacc ccaagtgact tgatccgtgc ggcagattcg gctttatatc
     1261 atgccaagaa atatgcccgc ggcgagtatg tggttgccaa ggcgatcacg ggtaggttga
     1321 atccggttca agtgaaagat ggttcctgaa gcgagaggca actcaaagtt acgaagttga
     1381 ggcggtgggg ttatatcctt aaaccaataa ggagagacaa atgcctcgta aagtcaaatt
     1441 gatcctcaac cctatggcag atatggggcg ggcgtggaag accgccaatg cgttgcgacc
     1501 gattgctcag gaatttcaag gtgagcttac ctggagcggg acggtgtacc caacacacgc
     1561 gattgaactt gcgcggcagg ctgccgaaga gggatatgac atggtgattg ccatgggcgg
     1621 ggatggtacg gttcatgaag tgatgagcgg gttgatgcaa gtgcctgccg agaagcgccc
     1681 tgtgatgggc gttgtgccga ttgggtctgg aaatgatttt gcgtattcta ttggtatcca
     1741 ccaaaagcca gaccatgcgc ttgcccatgc cttgaaggca gaaaatatta aacctgtgga
     1801 tatagggctg atgacggatg aacacgggcg caaggaatat tttgataata cccttggcat
     1861 cggttttgac gcggtggtaa ctatccgttc gcataagctg ccgattgtca aaggatttct
     1921 gatgtacttg accgcggtaa ttcaaaccat tcttttgaat cataaccctg caaaattgaa
     1981 tattgaaact gatcatcaat cgtggagcgc aagcgctttg atgctgacgc tgtgtaacgg
     2041 tccgcgagag ggtggtgggt ttatgctttc gccgaaatca aagaacgatg atggcacaat
     2101 ggaatttttg attgtcaata aggtttcgcg tgcgatgatg tttcgattgg tgcccgagtt
     2161 tatgagcgga acgcacatgc gttttaaaca gatccaaatg ggcgaattta agagattttc
     2221 gctcacttct gattctccgc tttacattca tgcagacggt gagattttta caagttttgg
     2281 cagtaatcta aaaaaggtgg cattcgaaat tctgcctcat gcattgaaag tggtgcatgg
     2341 gtaaaaaagg agaggcgacc tacaagtcgc ctctcctttt ttattctatt gctttcaaga
     2401 attcgtctct cttcgttacc gtttctttga gccctagggc tttccctaag cgggtgagtt
     2461 ggggcgggtc gacataggtt tgtgaaagga tttcttcctg acccagtaca tcgcgagcta
     2521 tgccatctag taagatttgt ccctttgaga gagcaatgac acggtcgaag ttctccgcac
     2581 agaaatcgat gtcgtgggtg atggtgatga cagttttacc ttcacgcttt agttctgcaa
     2641 tgacatgagc aatgcgaccc acgttgactg catcttgccc ggtggtaggt tcatcgaaga
     2701 tgacaatggg ggtatccata gcgatgatgg atgccagagc aaccattttg cgccaagtag
     2761 gggaaaggtc gtaaggattg gtttcggtct tatcactcaa ctcggttaag gataaagcgc
     2821 gctttacgag gtcgtccact ttttcttttg cataccccaa attgcgcgga ccaaatgcca
     2881 cttcggtcag gacgtctcta gagaataatt gttcatcggg gttttgaaat acatagccaa
     2941 cacgggatgc cagtttggcg gttgaatatt tggaggtttc ccaatcgccg atcaaaactg
     3001 tacctgaact ggggcgtagt aagccattga agtgacgcac taatgtggtc ttgccagccc
     3061 cattctgacc gacaatggca acttgttccc ctgattggat ggtgaggttg atgcctttga
     3121 gggcttctaa cccggtggga tatgtaaagc ggaggtcgtt gatctcgatg ttcatggttg
     3181 attatttgtc atcatgcaga ttaggcatga cgcttaaacc cttccaccgc ctcatctaac
     3241 gtgacgggta atgtttcctt tttccatagt ccgcgtttct ttgcttctcg cgctgtggat
     3301 gtataccggg aaatgccaaa gccgctttct gccaatgtgt ctgaagtcag cacttcgttc
     3361 ggcttgcctt ccaaaaggat ttgtccttct ttcaaagcga tgacgcgatc tgcaaactcg
     3421 gcgatccatt ccactttgtg ttcgaccatc acaacggtca tgccttcttc cgccattttg
     3481 cgcaccacgc caaagacttc gcgggtgcca atgggatcca tttgcgaggt gggttcgtcg
     3541 agcaccaaaa gcttgggtcg catgaccagg atcgaggtca gcgcgacgcg ttgttgctgt
     3601 ccgccagaga gagagtaggg agaccgatcg gctaactcgc tgattccggt cagggacatg
     3661 gcatcctgta cgcggctttt gatttcttca cgcggcacac cgatgttttc caacccgaaa
     3721 gcaatctctt caaagaccgt atattttgcg ccggaaattt gattgaaggg attttgaaac
     3781 gccaagccaa catttaagac ccattcgtct aatgtggact tggaggaggc ttttccactt
     3841 acttcgattt cgcctgaaaa ttcgccttta aaaaagtgcg gcgcaaaccc agccaatgca
     3901 tagcaaaacg tggatttgcc tgcgccattt ggaccaacga tggaaacgaa ttcaccttct
     3961 tgaatctgtg ccgtaatgtt tcgtagcacc ggctctttga caagtggata tttgtatgtg
     4021 aggttttgaa tgtttacgaa agccatagat tcaatccaat ggaaatgacg attaatgcga
     4081 gcaagcccca ccgtaaagtt ttttcagagg ctgtatctgg aatttcatgc aaagaggttt
     4141 tgacctgttt ggatgtaaag ccgcgcgctt caatggcgat agcacgttct tccacttcga
     4201 ctaaggagcc gaacacaagc ggaccgatca gcggaatgag agacccagcg cgtttgagaa
     4261 agctgctttc tgtgtcgagt ccgcgtgagc gctgagcgga gatgatggtt tgtgctttgg
     4321 cttgcatttg cggcaaaatt tgcagtgtag agatgatgac gtaagcaaac tgaccgggca
     4381 atccacgccg agtaaggtcg gacattaact cactggggtg tgtagttaac aaaaagatgg
     4441 tgaaacctga aaccatgacc acaatgcgca tggcattgcg gaaggcgaac atgac
//
LOCUS       DAOREF010000119         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11317_length_4467_cov_3.276065, whole genome shotgun sequence.
ACCESSION   DAOREF010000119 DAOREF010000000
VERSION     DAOREF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11317_length_4467_cov_3.276065"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKI33_03570"
     CDS             complement(<1..302)
                     /locus_tag="PKI33_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36112.1"
                     /translation="MQKKILSLLLFMWVLSACGSSASEPQVITASGDITAAVEQYRAL
                     LGENNGGDPGTKGSSGYREINWDSLPDEESAPNLYAPDFFNQPEAPRARGIVLNTAG"
     gene            complement(397..1269)
                     /locus_tag="PKI33_03575"
     CDS             complement(397..1269)
                     /locus_tag="PKI33_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNM36113.1"
                     /translation="MSHPNLFANLLGSGYIIQLMLHGYRLTPHGRIALNFNASSLDEL
                     TGRLSDGFYTTFSTLAQGTKVLGLKAHFDRLYQPAKELGLHPSADEQTVRKEIVDLAK
                     ALLPQESRVRLILTKDTGDVYLGIQPLIPPSKAVYENGVHVITAEMARHDPRIKGTDF
                     ITQSAEQRKLVKDDVYEILLTKNGKVLEGMTSNFYAIKGKTIITARNGILLGVTRRAV
                     LKLARGEGMSIQYRSPLVGEKFDEAFLTSSSRGVVPIVQIDGTSVGQGKVGAWTKRLS
                     LAYQAYVEARSESI"
     gene            1309..3741
                     /locus_tag="PKI33_03580"
     CDS             1309..3741
                     /locus_tag="PKI33_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36114.1"
                     /translation="MSADKSNITRASELTAANLRSVAKTISAQQLSRLTLPEIEAVVQ
                     LVSKIMPAGNVPGMILSGLARLPGRRIPVQKLQQDVTALFSGVEQILDSAVYGAFFAG
                     PAAVLWGYQNLLKLAGKDPESAFPEGMWQFYVDYALREDTARHMNETHGFDTILAQHN
                     IKLDKVDRLTAWFMAAVACLHQYPALLENEWRERVAISLLEKTLRDAGIESQRAKKIH
                     REWEIKRPYRREADGAQYDYPSYRRKKFDEFLQSRLQTMPELVYAAWEAGFNTALEQG
                     LQAYQRQMSIVAYLDPGPYGETRVPFDLSDAKIGIIHKDSYFLLPVCDEIGNPLDVMT
                     VRAQIASLLNSAFVSPSQISSLAKIKRSALAGLRSKLNPVLVNDLDNLKFAPILISTD
                     THSRALPLSELRQTERGVGSHALTIFDTGESFVFDQSHIFFDGTWGTALSEIMTNEAL
                     SWARYLSLLPSPVAPEMRLYTSLTLQLSPADLDLVQQAPKVMPEVGAETDKIDLKACM
                     SLRKDFKQRNDRIELTINDLLVLYRAVHAATYVPSQLVTNEIQKLSTVKPDVAASLHQ
                     LVEEGSRTNPAILIPMDASLKTPRDRVYPMNVEVPLADLNLLAMHAQTLNILEAYESA
                     SGGDRAVIFASFNKFQKAYLSTIAGFGAYLTRAKEMAAQGENTAAGAIKLLAHLPLPI
                     QRLLDKIPERFEVLNNLIKGREVFSNVGAVVPSSTLTRFMTAKDDNAQKQLAWGIITD
                     AKSVLRIHLRDFRPHVQALSSIGRKDLASLITQDYLNAYAEGFNQYIRDLSRITTASR
                     ETMTKPQAKGNS"
     gene            3744..>4467
                     /locus_tag="PKI33_03585"
     CDS             3744..>4467
                     /locus_tag="PKI33_03585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM36115.1"
                     /translation="MKDPMIGMQLANFKVESLLGQGGMAMVYYGQDVKLHRPVAIKVL
                     DKRYKNHPAYAGRFVNEARMMAKWHHENIIQIYYADDTQGFPYYVMEYVDGQDLGSVI
                     DSYQSDGNFMPHNEVLRIGKAIASALDYAHRHGVVHRDVKPSNILLSKDGRVLLGDFG
                     MALEVRDGSMGNIFGTPHYISPEQAKRSADAVPQSDIYSLGVIMYEVLAGATPFNDSS
                     PANIALQHISQKPPAPSTINPNL"
BASE COUNT         1150 a         1102 c         1049 g         1166 t
ORIGIN      
        1 cctgctgtgt ttaagacaat gccacgtgct cgcggcgctt caggttgatt gaaaaaatca
       61 ggcgcataaa gattaggcgc tgattcttca tctggcaggc tgtcccaatt aatttcgcga
      121 tagcccgaag atcccttcgt gccggggtcg ccaccgttgt tttcacctag caatgcacga
      181 tattgctcta cagccgcggt aatatcgcca gatgcggtaa tcacttgcgg ctccgaggcg
      241 ctgcttccgc aagcggagag cacccacata aacaataaca aactcagtat ttttttctgc
      301 atctcgaact cctttgtcca ataaaaatag atagcgcgat tatatggatg tcgcgctatc
      361 tatcccaccg actttagtga gatacaggtt ctaactttag atgctttcgc ttctcgcttc
      421 gacataagcc tgatacgcta aagagagacg ttttgtccac gccccgacct tcccctgccc
      481 cactgacgtc ccatcaattt gcacaatcgg caccacgcct cgcgaactgg atgtaagaaa
      541 ggcttcgtca aatttttcac ccacaagcgg agaccgatac tgaatagaca tcccctcccc
      601 tcgcgcaagc tttagcacgg cacggcgtgt cacccccagt aagattccgt ttcgggctgt
      661 gatgatcgtc ttgcctttga tcgcataaaa attggaagtc atgccttcca agacttttcc
      721 atttttggtt agcaaaattt catagacatc atcttttacc aatttgcgtt gttcagcgct
      781 ttgggtgatg aagtccgttc cctttatacg tggatcatgg cgagccattt ctgccgtgat
      841 aacatgcaca ccattttcat acaccgcctt agaaggcgga atcaatggtt gaatgccaag
      901 ataaacgtca cctgtgtctt ttgtgaggat aagccgaacg cgggactctt gtggcaagag
      961 cgcttttgcc aagtccacga tctctttgcg gacggtttgt tcgtctgctg agggatgcag
     1021 ccctagttct ttcgcaggtt gatataacct gtcaaagtga gctttcaagc caagcacctt
     1081 tgtcccttgc gcgagggtgc taaaggtggt gtaaaagcca tctgaaagcc gacccgttaa
     1141 ctcgtcgaga gaagaggcgt tgaaattaag ggcaatacgc ccatgtggtg taagtcgata
     1201 tccatgcaac atgagctgaa ttatatatcc gctgcctaac aaatttgcaa acagattagg
     1261 gtgagacaaa aaaatccatc aagatgaagg tgaaatataa tttcatagat gagcgcagac
     1321 aaatccaaca ttacacgtgc ttcagagctc actgctgcta atttgcgctc ggttgcaaag
     1381 accatttctg cgcaacagct ttcacgcctc acattaccag agatcgaggc ggtggtgcaa
     1441 ttggtctcca agattatgcc ggcagggaat gtgcccggta tgatccttag cgggctggca
     1501 cgtttgcccg gtcggcgtat ccccgtccaa aaattacaac aagatgtaac tgctcttttt
     1561 agcggtgtag aacaaatttt ggatagtgct gtgtatggtg cgttctttgc cggaccagct
     1621 gctgttctat gggggtacca aaatctgttg aagttagccg gcaaagaccc tgagtctgcg
     1681 ttccccgaag gaatgtggca attttatgta gactatgctc tgcgtgaaga tacagcccgt
     1741 catatgaacg agacgcatgg ctttgatacg atccttgcac agcataatat caaactcgat
     1801 aaagttgacc ggctgacggc ttggttcatg gctgcagttg cttgtttgca tcaatacccg
     1861 gcattgcttg aaaatgaatg gcgtgaacga gttgccattt ctttgctcga aaaaacgttg
     1921 cgcgatgctg gaatagagtc acaacgcgcg aaaaagatcc accgtgagtg ggaaataaaa
     1981 cgtccttatc gtcgtgaagc ggacggcgcg caatatgact atccatccta ccgacgtaag
     2041 aagttcgatg aatttctaca aagccgactg caaaccatgc cagagttggt gtatgcggca
     2101 tgggaagctg gcttcaatac ggcgttagaa caaggcttgc aagcatatca acgccaaatg
     2161 tcgattgtgg catatctcga ccccggacct tacggtgaaa cgcgtgtgcc attcgatcta
     2221 tcagatgcaa aaattgggat tatccataaa gatagttatt tcttgctgcc cgtctgcgac
     2281 gaaattggca acccgttgga tgtaatgacg gttcgagcgc agatcgcttc tttattgaac
     2341 tctgcatttg tttccccgtc tcagatttca tctctagcga agatcaaacg ctcggcatta
     2401 gcagggctac gctcaaaact taacccggtt ttggtgaacg acctggataa tttaaaattt
     2461 gcgccaattc tgatctctac agacacacat tcgcgggcat tgccactttc ggagttacgt
     2521 caaacggagc gcggcgtggg ttcccatgcg ttgacaattt ttgatacggg cgagtctttt
     2581 gtttttgacc aatctcacat cttttttgac gggacttggg gcacagcact ttctgagatc
     2641 atgaccaatg aagccctttc atgggcaagg tatttgagtt tgctcccctc ccctgttgca
     2701 cctgaaatgc gtttgtacac ctctctcacg ttacagcttt ctcccgccga cctagacctt
     2761 gttcagcaag caccaaaggt tatgccagaa gtcggcgccg aaacagataa gattgacctg
     2821 aaagcctgca tgtcgctccg caaagacttt aagcaacgga atgaccgcat cgaattgacg
     2881 atcaacgacc tgcttgtgtt atatcgcgcc gttcacgcgg cgacatatgt cccttcgcaa
     2941 ttagtcacga atgaaattca aaagctttcc accgtcaagc cagatgttgc cgcatcctta
     3001 catcaattgg tggaggaagg cagccgtaca aatcctgcta tcctcatccc tatggatgcc
     3061 agcctgaaaa ccccgcgtga tcgggtgtat cctatgaatg tagaagttcc gcttgcggat
     3121 ttaaatctgc tcgcgatgca tgctcaaacg ttgaacatac tggaagcgta tgaatccgct
     3181 tcgggcggag accgagccgt gatcttcgcc agtttcaata aattccagaa ggcatatctc
     3241 tccacgatcg ctgggtttgg cgcttacttg acccgcgcca aagaaatggc agcacaaggc
     3301 gaaaacacag cggctggggc gatcaaattg ttagcacatt tgcctctgcc tattcaaagg
     3361 cttttagata agattccgga gcgctttgag gtactcaaca acctcatcaa agggcgcgaa
     3421 gtattttcga atgtaggcgc cgtagtcccc tcaagtacgc ttactcgctt tatgacagca
     3481 aaagatgata acgcccaaaa acagctcgct tggggtatca tcaccgatgc gaaatcggtt
     3541 ttgcgaattc atctgcgcga ttttcgtccg catgttcagg ctttgtcatc cattggacgc
     3601 aaagaccttg ctagcctcat tacgcaagat tatttgaatg cctatgctga aggattcaat
     3661 caatacatcc gcgatctttc acgtattaca actgccagtc gagaaactat gactaagcct
     3721 caagcaaaag gaaattccta gccatgaaag atccgatgat cggaatgcag ctcgccaatt
     3781 tcaaggtcga gagcctgtta ggtcagggcg gtatggcgat ggtctattat ggacaggatg
     3841 tcaaactgca ccgtccggtt gccattaaag ttcttgataa acgctacaaa aatcaccctg
     3901 cttatgcagg gcgtttcgtc aatgaagcgc ggatgatggc aaagtggcac catgagaata
     3961 tcatccaaat ttattatgcc gacgacacac aaggtttccc atactacgtc atggagtacg
     4021 tggacgggca agatctgggc tctgtcattg attcgtatca atcggatggg aacttcatgc
     4081 ctcacaatga agtactgcga atcgggaaag cgattgccag tgcattagat tatgctcaca
     4141 gacacggggt tgtgcatcgc gatgtgaaac cctcgaatat tctgctctcc aaagatgggc
     4201 gcgttttgct cggcgacttt ggcatggcgc ttgaagttcg tgatggatcg atgggaaata
     4261 ttttcgggac gcctcattac atatcgccag aacaggctaa acgttcggca gatgcggttc
     4321 ctcaatctga tatttattct ttaggcgtta tcatgtatga agtccttgcc ggagcaacac
     4381 cattcaatga ctcttcgcca gcaaatattg ccctgcagca tatttctcaa aaaccgcctg
     4441 cgcctagtac gataaacccg aacttgt
//
LOCUS       DAOREF010000120         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11345_length_4459_cov_4.833787, whole genome shotgun sequence.
ACCESSION   DAOREF010000120 DAOREF010000000
VERSION     DAOREF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11345_length_4459_cov_4.833787"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKI33_03590"
     CDS             complement(<1..379)
                     /locus_tag="PKI33_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HNM36116.1"
                     /translation="MMVRKSKGIGERHQKILDFIASYQREHKHPPSIREIGEHCDISS
                     TSVVNYYLDQLEKEGLLERDRKISRGMRLVNNSPLGDMLRVPVMGVIQAGEPIPIPAS
                     DFNPFTAEDTVDIATSLMPSKEKG"
     gene            complement(502..1488)
                     /gene="corA"
                     /locus_tag="PKI33_03595"
     CDS             complement(502..1488)
                     /gene="corA"
                     /locus_tag="PKI33_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNM36117.1"
                     /translation="MIRSLYYKPGQPIRTNIPPTEFPRLIRDRKGTLWVDFIGEPEEL
                     AEPILRGFGFHPLAIDDALQETHTPKVDDWGDYIYLVLSILNYKQENGMFEAEIDELD
                     VFLGHNYVVTFHDQLLTAVEDTWIACQRDIRHVQDGADHLLYRIVDSLVMSYMPLVEQ
                     IDAQIDQIEDQVFDKPRRDILEQIFALKRLLLTMRRILLPQREVLNKLARDDFRVIDP
                     RDRVFFRDIYDHLVRLHDLNESLRDLVSGALDTYLSVTNNRMNEIMKVLTIITTFFMP
                     ITFITGFFGMNFFEPVAHLVGWTSQQVFAGMMSVMIGLPILMYFWMRGRTWL"
     gene            1710..2093
                     /locus_tag="PKI33_03600"
     CDS             1710..2093
                     /locus_tag="PKI33_03600"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNM36118.1"
                     /translation="MRPAPTRCPICQSEMEVVRLHCSSCDTSIEGSFALGQFSNLSPE
                     QMEFVFTFVRCEGKINRMEQELGLSYPTIRNRLHEVIRKLGFEPGRDETPEVDDETRS
                     SVLDDLESGKITADQAMRILRGEEE"
     gene            2097..3194
                     /locus_tag="PKI33_03605"
     CDS             2097..3194
                     /locus_tag="PKI33_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36119.1"
                     /translation="MSKTISAGRTPRIEITAVSGDLSLVGWEGDDILLKADDEEMNVK
                     QDGDQVTIACKDDLSMRVPKGATVIIHRITGDASVRGVMGAIELKEISGDLSMRDVVT
                     VAIDTVHSDFSLRGAKGSLSIRKAGSDVSVRDVDGNVAIESVSDDLALRDVRGSVTAN
                     VGEDVVMYLNPQAGNTYAVNAGDDILLVMPPKANATLTLSADEIDVDWDGVENEEDAT
                     SRVIILGDGSANLTLSAGGDIRVSNRSDAGESAEDFGNFAGMGMDWSGFGERISRRVE
                     QATSRAQRKAEEAARRAENKMRDAERRGRGKVAFGVGRWNWDFSPKGVPVPPKQQVSD
                     EERLAILKMLQEKKITAEEAEKLLAALEGGS"
     gene            3194..3856
                     /locus_tag="PKI33_03610"
     CDS             3194..3856
                     /locus_tag="PKI33_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36120.1"
                     /translation="MSSEERRKILQMVADGKISAEEAATLMRALDDSNAEEASAEMEI
                     IEVRPASSAEGAGSSYQEKSHAPELDAVRMRAHRFSGAFLWIGTIVTVLIAWWMFNVQ
                     QNSGLNFWFFCLSLPLTLGILLIALGAGSRTSRWLYVNVDRSQSKDQDGPRKISLAFP
                     LPLGFASWFLRTVGRHIEELRDKNVDAIADTIALAKNMTEPLIVHVDDDSDGTQVQVF
                     IG"
     gene            3863..>4459
                     /locus_tag="PKI33_03615"
     CDS             3863..>4459
                     /locus_tag="PKI33_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36121.1"
                     /translation="METIKKQPLLSGILILFLTAMIFANIGGNMYEGLMPLYLKELKA
                     DITDIGLFFTLAQIVPLFLQILGGWVSDSLGRLRAIAIGSVFGLLGFIPLVLADTWHW
                     LLIATAVGAIARALVGPSFDAFIAEHSTEENRGKVYGISQAIFMIVTVVGPPLGGWLT
                     GAYGFKLMLFIAGVFYAIAVAMRLSMAREATKGEKENGE"
BASE COUNT         1130 a          928 c         1212 g         1189 t
ORIGIN      
        1 tgcctttttc ctttgaaggc atcagagagg tggcaatatc cacggtgtct tctgcagtga
       61 aggggttgaa gtctgaagcg gggattggaa tgggttcacc agcttgaatg acgcccatca
      121 ccggtacgcg aagcatatcg ccaagcggac tgttatttac caaccgcatg ccgcgcgaga
      181 tcttgcgatc acgctcgagc aacccttcct tctccaattg gtctaagtag taattcacga
      241 ccgaagtgga ggaaatgtca caatgctccc caatctcacg aatggaaggg gggtgcttgt
      301 gttcgcgctg gtaagacgca ataaaatcca gaattttttg gtggcgttcg ccgatacctt
      361 tactttttcg aaccatcatc atttcaagcc tcctgccagg ggactggcgt aggtacagag
      421 catttttcgc tcatttgtgc tagtaataat gataaccgaa cgcctgttct gtgtcaaggc
      481 attttggggt gcaaaatgga gctataacca ggtccgtcca cgcatccaaa aatacatcag
      541 gatgggaagc ccgatcatga ctgacatcat accggcgaaa acttgttggc tggtccaacc
      601 cacaaggtgg gcaacaggtt cgaagaaatt catgccgaaa aagccggtga tgaaggtaat
      661 gggcatgaaa aaggttgtaa taattgtgag gactttcatg atctcattca tgcggttgtt
      721 ggtaaccgaa agataggtat ccaacgcgcc gctgacaagg tcacgtaagc tctcgttgag
      781 gtcatgcagg cgaacgagat gatcgtaaat atcgcggaag aagacacggt ctctggggtc
      841 gatcacgcgg aagtcgtcgc gggcaagctt gttcagcact tcacgctggg ggagcaggat
      901 ccggcgcatc gttagaagga ggcgtttgag agcaaaaatc tgttcaagaa tgtcacgccg
      961 cggcttgtcg aagacttgat cctcaatttg gtcaatttgg gcgtcaattt gctctacaag
     1021 cggcatgtag ctcatcacaa gcgagtcgac gatgcgatag agcaggtgat ctgctccgtc
     1081 ttgcacatgg cgaatatctc gttgacaggc gatccaagta tcttctaccg cggtcaataa
     1141 ctggtcatga aaagtgacca catagttatg cccaaggaat acatccaatt cgtcaatttc
     1201 tgcctcaaac attccatttt cttgcttgta gttcaaaatg ctcaacacaa ggtagatgta
     1261 atcgccccaa tcgtccactt tgggggtgtg ggtctcttga agggcatcgt caatggctag
     1321 cggatggaag ccaaaaccgc gcagaatggg ctctgctagc tcttcaggct ccccaatgaa
     1381 atctacccaa agagtgcctt tccggtcgcg tatgaggcgc ggaaactcag taggggggat
     1441 gtttgtgcga atgggctgac caggtttgta gtagagagag cgaatcaaga gtgacctcca
     1501 gggacgactt taatatttta aagctaaact caataatctt caagttgata ttgattttct
     1561 gtagaatcta ttgacaaaag ttaaatctgt tgtataattt tattagaaat caggataaat
     1621 ttattgattt ttcgaaaaaa gtccttaaaa atatcgattt tcggctaaaa aattactttt
     1681 tgacacatta taaatcggag aagcctgtta tgcgacctgc cccaacccgt tgcccgatct
     1741 gtcaatctga aatggaagtt gttcgtttgc actgctccag ctgtgatacc tcaattgaag
     1801 gtagctttgc tctggggcag ttttcgaatc tctctcctga acagatggaa tttgttttca
     1861 cctttgtgcg ctgcgaaggc aagatcaatc gcatggagca ggagttgggg ctttcatacc
     1921 caaccattcg caaccgcttg cacgaggtga ttcgcaaact tggttttgag cccggcagag
     1981 atgagacccc tgaagtggat gatgaaaccc gcagcagtgt tctcgatgat ctcgaatccg
     2041 gaaagatcac agcagatcaa gccatgcgca ttttgcgcgg cgaggaggaa taagctatgt
     2101 ctaagaccat ttctgcagga cgcaccccaa ggatcgaaat tacagcagtc agtggtgatt
     2161 tgagcttggt agggtgggag ggagatgaca ttctcctcaa ggcagatgac gaagaaatga
     2221 acgtcaagca agatggcgat caggtcacta ttgcttgtaa agatgatctt tcgatgcgcg
     2281 tccccaaagg ggcgaccgtc attattcacc gcattacggg tgatgcttct gtgcgtgggg
     2341 tgatgggagc gattgaatta aaggaaatct ctggtgacct ttccatgcgc gatgttgtga
     2401 ccgtggcaat tgatacggtt cattcggact ttagcctgcg tggtgcaaaa ggaagtttat
     2461 ctatccgtaa agcgggctca gacgtttcgg tccgcgatgt ggatggaaat gtagccattg
     2521 aatcggtttc agatgacctt gctttgcgtg atgtgcgcgg tagtgtcact gccaatgtgg
     2581 gtgaagatgt ggtcatgtat ttgaatccgc aggcagggaa tacctatgcc gtgaatgcgg
     2641 gcgatgacat tttattagtg atgccgccca aagcaaatgc gaccttaact ctcagtgctg
     2701 acgaaatcga tgtggattgg gatggcgtag aaaatgaaga agatgccacc agtcgcgtga
     2761 tcatccttgg cgatgggtct gccaacttaa ctttgagtgc aggcggcgat atccgcgttt
     2821 cgaatcggtc tgatgctggt gaatcggctg aagattttgg caacttcgcc ggcatgggca
     2881 tggattggtc tggttttggc gagcgtatct cgcgccgtgt ggagcaggca acgtctcgcg
     2941 cccaacgcaa ggcagaagaa gccgcccgcc gtgcagaaaa caaaatgcgc gatgcagaac
     3001 gacgtggacg cggcaaagtg gcttttggcg taggacgttg gaattgggat ttctcaccta
     3061 aaggggtgcc ggttccaccg aaacagcaag tttcagatga agaacgtttg gcaattttga
     3121 agatgcttca agaaaagaag atcaccgcag aagaagcaga aaaactttta gcagcacttg
     3181 aaggaggttc ctaatgtctt ctgaagaacg cagaaaaatt ctgcaaatgg ttgcagacgg
     3241 aaaaatcagt gcggaagaag ccgctacctt gatgcgtgca ttggacgact ctaacgctga
     3301 agaggcttca gcggagatgg agattatcga agtgcgcccc gcttcgagcg cggaaggagc
     3361 cgggtcttca tatcaagaaa agagtcatgc ccctgaatta gatgctgttc gtatgcgcgc
     3421 tcatcggttc tctggcgctt tcctttggat cggaacgatc gtaacggttt tgatcgcgtg
     3481 gtggatgttc aatgttcagc aaaattctgg gttgaatttt tggttcttct gtctaagctt
     3541 gccattgacc cttggtattt tattgatcgc cttgggtgcg ggcagccgta catcgcgttg
     3601 gttatatgtg aatgtagatc gttcgcagtc aaaggaccaa gatggtccgc gcaagatttc
     3661 attggcattc cctttgccac ttggttttgc aagttggttt ttgcgaacag tgggacgaca
     3721 tattgaagaa ctacgcgata agaatgtaga tgccattgcc gacacgattg ctttggctaa
     3781 gaatatgacg gaaccgttga tcgtacatgt ggacgatgac agtgatggta cgcaggtgca
     3841 agtttttata ggatagaaaa aaatggaaac catcaagaaa caacctttat taagtggcat
     3901 attgatcttg tttctaacgg caatgatctt tgcgaacatt ggcggaaata tgtatgaagg
     3961 gctgatgccc ttgtatttga aggaactgaa agcagatatt accgatatcg gtctgttctt
     4021 tacattggct cagattgtgc ctttgttcct tcagatttta ggtggttggg tttcagattc
     4081 tttaggacgt ttacgtgcca ttgcgattgg tagtgtgttt ggtcttttag ggtttattcc
     4141 gttggtgttg gcagatacct ggcactggtt gttgattgct actgctgtag gtgcgattgc
     4201 tcgtgctttg gtcggaccca gctttgacgc tttcattgcg gaacactcca cagaagaaaa
     4261 ccgtggcaag gtgtatggta tttcgcaggc gatctttatg atcgttaccg tggtcggacc
     4321 tcctttaggt ggctggttga ccggtgccta tggcttcaaa ttgatgttat ttattgcagg
     4381 tgtgttttat gctattgctg tagcaatgcg tttgagcatg gcgcgcgagg cgacgaaagg
     4441 cgaaaaagag aatggcgaa
//
LOCUS       DAOREF010000121         4436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11464_length_4436_cov_3.406300, whole genome shotgun sequence.
ACCESSION   DAOREF010000121 DAOREF010000000
VERSION     DAOREF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11464_length_4436_cov_3.406300"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..925)
                     /locus_tag="PKI33_03620"
     CDS             complement(86..925)
                     /locus_tag="PKI33_03620"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNM36122.1"
                     /translation="MKYSLNAREGDLAQLVGLTHKHFIFMLQAGGDFQSHRGVIKHDD
                     LIGKPWGSQIFSHTGAPFFLLQPSMSDILNELPRNTQILYPKDIGYILIQMGISEGQT
                     VLEAGTGSGSMTISMATAVGPNGRIVSYEKTPDTQNLAKKNLQRIGLASRVDFKLRDI
                     QEGFDETDADAFFLDVQNPFDYIQQVRATLKPGGFFGTLVPTYNQVEKVLYALKKHNF
                     AFVEVCELMLRYYKTNPARLRPTDRMVAHTGYLIFGRKIEPSEDPRGAALAKEIIGIE
                     EEY"
     gene            complement(953..2221)
                     /locus_tag="PKI33_03625"
     CDS             complement(953..2221)
                     /locus_tag="PKI33_03625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNM36123.1"
                     /translation="MQTKKIHWASLVILLILATSITFLLLIVLSLGVASLVDLFSSSA
                     EAASEMIGAVAFGFISILLLISGWFILQKTRGLEQADQNFKFPFAPWMWLVLPALVIF
                     SILFGGLITFAEIRWLNWLLLPFLTVLVIVPPIWLLFGIASNGLNLGPRWRFFSILGL
                     GMTAGPIIMIVLEMVLLLILIIAGVAYIAITRPEMVSEIESLANMLNQGLSEDAMLNL
                     LSPYLTNPTLLAIGIGYIALLVPLIEELFKPLAVWLFARQLESPAQGFALGVLSGAAF
                     ALVESLNASADGTTSWAVIVAARTGTSVLHMLTTGLVGWGIAMAFKEKRFGRLVAAYL
                     SAVLIHGIWNAAAAGTGISALGESVGKPEWLFNYAPALVCGLLVLGIGTTSLLVASNR
                     KLRKQLELAPAVDSSVESTPVQETPETPSE"
     gene            complement(2221..3456)
                     /locus_tag="PKI33_03630"
     CDS             complement(2221..3456)
                     /locus_tag="PKI33_03630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNM36124.1"
                     /translation="MKRILFGLVFFGIITLFFTACSTENTPTPYIVLDPVIPPTPTPQ
                     YTCTVISAMPTLSPNEQSLAPAITAADFSFGPSDAPVTILEYCDFQSDGCLAMAQTIA
                     ELMRNYENQIRFVFRPLPLSTVLDKSDLSALAAFAAGEQGKFWEFYDLLFSKHGEWKN
                     LSPGEFKGWLTRETPAIGIDASQLTAAMEAEGALTQLKSAQEAMTQLYITAVPVILIN
                     GSLQQSYVLDYRNMYDTVGLILLGEKQFTECPPYTVDASKQYIATIETAKGNIVIQLF
                     PDKAPLAVNSFIFLAKQGWYDGVTFHRVIPGFAAQAGDPSGSGRGNPGYFFNNEPNDL
                     VFDKPGLVGMANSGPDTNGSQFFITFAPAEHLNGAYTIFGQVISGLDVAEQLTARDPS
                     QGGFLPAGDLILHITIEEK"
     gene            complement(3459..>4436)
                     /locus_tag="PKI33_03635"
     CDS             complement(3459..>4436)
                     /locus_tag="PKI33_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36125.1"
                     /translation="YADPSIQDKDAATETVRVDLAELYKRQEQIAPLAEAILQDQVTQ
                     VLVEQGLTVGGQPIPNVWYHSTPLPMALIISPRDHIEQTANISVNTDLTVDEQVKLEE
                     DVAKELDVSSLVVQIGGVGVYPTMVARTTNLNWLLSTIAHEWIHNYLTQRPLGLLYGE
                     SPELRTMNETTASIAGDEIGALVIEKFYPELKPAASFPNLSLISLSFDRPDPETLERP
                     PFDFRAQMHETRVTADALLAAGKIDEAEAYMEARRQIFLQNGYLLRKINQAYFAFHGA
                     YADVPGGAAGEDPVGPAVRALREQSPSLKDFINTIAWMWSFEQLQETIK"
BASE COUNT         1121 a         1063 c         1182 g         1070 t
ORIGIN      
        1 cattaagcga tgggcatttc gtaaggcagg cggcaccgcc gggacgggcg ctccgcgtaa
       61 taatcttctg gcgcgtctaa acatcttagt actcctcttc gataccgata atttctttgg
      121 cgagtgctgc tccgcgtggg tcttcgcttg gttcaatttt tcgtccaaaa ataaggtagc
      181 ccgtgtgcgc caccatacgg tctgtgggac gtaagcgggc agggttggtc ttgtaatagc
      241 gcagcatcaa ttcgcagact tctacaaaag cgaagttatg ttttttcagg gcatacaata
      301 ctttttcgac ctgattatag gtaggaacga gcgttccaaa gaatccgccc ggcttgagcg
      361 tggcgcggac ctgttgaatg tagtcgaacg ggttttgcac gtcgaggaag aaggcatcgg
      421 catccgtttc gtcgaagccc tcttggatgt cacgcagctt gaagtccacg cgagaggcga
      481 gaccgatccg ttgtagattt ttcttcgcca ggttttgcgt atctggcgtt ttctcatacg
      541 aaacgattct tccgtttgga ccaactgctg ttgccatgga tatcgtcatc gagccagaac
      601 ctgtgccagc ttcgagtacg gtttgccctt cagagatgcc catttggatg aggatataac
      661 cgatgtcttt ggggtacaga atttgcgtat tgcgcggcaa ttcattgaga atgtcggaca
      721 tggaaggctg aagcaggaag aagggcgctc cggtgtggct gaagatctgc gagccccacg
      781 gtttgccaat tagatcatca tgcttgatca caccacggtg actttgaaag tcgccgcccg
      841 cttgcagcat aaaaatgaag tgtttgtggg tcagccccac caattgcgcg aggtcgccct
      901 cacgcgcgtt aagagaatat ttcatgcagc gaattctacc tttactttcg atttattcag
      961 aaggggtttc tggggtttcc tgaaccggcg tggattcaac agagctatcc acagcgggag
     1021 cgagttcgag ctgttttctt aatttccggt tggaggcaac cagcaaagaa gttgtgccaa
     1081 tgcccaacac cagtaagccg caaacgagcg caggcgcata attgaaaagc cattcaggtt
     1141 tgccgaccga ttcacccaag gcagaaatgc ctgtgccggc ggcagcggca ttccaaatgc
     1201 cgtggatcaa cacagcagag agatacgccg caaccaaacg cccaaagcgt ttttctttga
     1261 acgccatcgc aattccccag ccaaccaagc ccgtggtgag catgtgcagt acactcgtcc
     1321 cagtgcgtgc tgccacaatg accgcccaac tagtggtgcc atccgcactg gcattcaagc
     1381 tttctactaa agcaaacgcc gcaccgctga gtacacccag agcaaaccct tgcgccggag
     1441 attcaagctg acgggcaaac agccaaactg ccaacggctt gaataattct tcaatcagcg
     1501 gaaccagaag cgcgatatac ccgatcccaa tggcgagcaa agttgggttg gtaaggtaag
     1561 gcgataacag attgagcatg gcatcttcgc ttaaaccttg gtttagcatg tttgccaaag
     1621 actcaatttc gcttaccatc tcagggcgtg tgatggcaat ataggcaacc ccggcaatga
     1681 tgaggattag caaaagtacc atttcgagca cgatcataat gatcggaccc gccgtcatgc
     1741 ctagcccgag aatactgaaa aaacgccaac gcggaccgag gttcagcccg tttgaggcga
     1801 tgccgaataa cagccagatc ggtgggacaa tgaccaagac agttagaaac ggaagtagga
     1861 gccaatttag ccatcgaatt tctgcgaagg tgatcaaccc gccaaataaa atgctaaaga
     1921 tcaccaaggc gggtagcacc agccacatcc acggggcaaa gggaaatttg aaattctggt
     1981 ctgcctgctc caatccgcga gtcttttgca aaatgaacca tccgctgatg agtagcaaga
     2041 tgctgataaa cccgaatgcc actgcaccga tcatttcaga agcggcttca gcagaactgc
     2101 tgaataggtc cacgagggat gcgacaccaa gcgacagaac gatcaaaagc aaaaatgtga
     2161 tgctagtcgc aaggataagc aatataacaa gagatgccca atggattttt tttgtttgca
     2221 ttatttctct tcaatcgtaa tatgtaagat caaatcccct gcgggcaaaa agccaccttg
     2281 cgaagggtcg cgcgctgtta actgttcggc tacatccaat ccactaatca cttgcccaaa
     2341 gatggtgtaa gccccattca aatgttcagc gggcgcaaaa gtaataaaga attggctgcc
     2401 attggtatcg ggtccagaat ttgccatgcc caccaaacca ggtttatcga acacaagatc
     2461 attcggctca ttgttgaaga aatagccggg gttccctcta ccgcttccac ttgggtcacc
     2521 ggcttgagcc gcaaagccag ggatcacgcg gtgaaatgtc acaccgtcgt accagccttg
     2581 tttcgcaaga aagataaatg aattaaccgc taacggggct ttatctggaa ataattgaat
     2641 cacaatattt cctttggcag tttcaatcgt ggcaatatat tgtttcgatg catccaccgt
     2701 gtaaggcggg cattcggtga attgtttctc gccaagcaaa attagcccta cggtatcgta
     2761 catattgcga taatccaaaa cataggattg ctgtaacgac ccattgatga ggatgactgg
     2821 aaccgcggtg atgtataact gagtcatcgc ctcttgcgcc gatttgagct gggtcaatgc
     2881 gccttcggct tccatcgccg ctgtaagctg actcgcatca atgccaatag cgggagtttc
     2941 tcttgtgagc cagcctttga attcgcccgg gcttagattt ttccactcgc catgttttga
     3001 aaataataga tcgtaaaatt cccaaaactt gccttgttct ccagcggcaa aggctgccag
     3061 tgcggaaagg tctgatttat ccaacacggt gctaagtggc agcggacgga acacgaagcg
     3121 aatctgattc tcgtaattgc gcataagctc ggcaatggtc tgtgccatgg caaggcagcc
     3181 atcagactga aaatcgcaat attctagaat agttactgga gcatcactgg gaccaaagga
     3241 aaaatctgct gcggtgatgg caggcgcgag agactgctca tttggggaga gcgtaggcat
     3301 ggcactgatc accgtgcagg tatattgagg cgtaggcgtt ggcggaataa caggatcgag
     3361 cacgatataa ggtgtaggag tattctctgt gctacatgcc gtgaaaaaca aggttattat
     3421 gccaaagaat acaagcccga agagaattcg tttcattatt atttgatggt ttcctgtaat
     3481 tgttcgaaag accacatcca agcgatggtg ttgatgaaat ctttgagtga ggggctttgt
     3541 tcgcgcaagg cacgcacggc gggaccaacc gggtcttcac cggcggcgcc accgggcacg
     3601 tctgcatagg cgccgtggaa ggcgaagtag gcttggttga tcttacgaag gagatagccg
     3661 ttctgtaaaa agatttgacg gcgcgcttcc atgtaggctt ctgcttcgtc gattttgccg
     3721 gctgctagta gggcatcggc agtgacgcgg gtttcgtgca tttgcgcgcg gaagtcgaag
     3781 ggcgggcgct ctaaggtttc ggggtcgggg cggtcaaagg aaagcgagat caagctcaag
     3841 ttcgggaaag aggcggcggg ttttaattcc gggtaaaact tttcgatgac gagcgctcca
     3901 atctcatcgc cagcaatcga tgctgtcgtt tcgttcatgg tgcgtaattc gggagattca
     3961 ccatataaca acccgagcgg acgttgcgtg aggtagttgt ggatccattc gtgagcgatg
     4021 gtgctgagta gccagttgag gttggtggtg cgtgctacca tggtggggta tacgcctacg
     4081 ccgccgattt gcacaacaag cgacgagaca tctaactcct ttgcaacatc ttcttcaagt
     4141 ttgacttgtt catcaactgt gaggtctgtg ttgactgaaa tattagcggt ctgctcgata
     4201 tggtcacgcg gcgagatgat gagcgccatg ggcaggggcg tggagtgata ccagacattc
     4261 gggatgggtt gcccgcccac ggtgagaccc tgttcgacca atacttgtgt gacttggtct
     4321 tgcaagatgg cttctgccaa cggcgcgatt tgttcctggc gtttgtacag ttctgctaaa
     4381 tctacacgga cagtttctgt agcggcatct ttgtcttgaa tggatgggtc tgcata
//
LOCUS       DAOREF010000122         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11482_length_4428_cov_4.563915, whole genome shotgun sequence.
ACCESSION   DAOREF010000122 DAOREF010000000
VERSION     DAOREF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11482_length_4428_cov_4.563915"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..494)
                     /locus_tag="PKI33_03640"
     CDS             complement(246..494)
                     /locus_tag="PKI33_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNM36126.1"
                     /translation="MSVTVNNRFAILLAEKRIKERRNISLAEVADEIGISRKTLYAWE
                     NNTVTRFDVPVIDALCQYFSVKPGDLFEYIEEEQPKKK"
     gene            633..992
                     /locus_tag="PKI33_03645"
     CDS             633..992
                     /locus_tag="PKI33_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36127.1"
                     /translation="MNSQNRLTKSEIFTVYNQMRAAARREGNLKLIERLNKAVGILLS
                     KEYYQGERLAYIPTAHSCGCHDWEFRNTAKRGYTGGCKHMLAEAMMQSALALRAAHEV
                     TVWLTPRESFPAEVAVR"
     gene            1322..1666
                     /locus_tag="PKI33_03650"
     CDS             1322..1666
                     /locus_tag="PKI33_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNM36128.1"
                     /translation="MAKKIFPLLVLLSAIAYGLVWLMPDVEPENASPIVIPSSQTLQS
                     PMPATPGDFCKVVTGLETGRVNLRECAGTACPVLRVLEDGQMLTVVKAGAWNEVTTVD
                     GVRGWINSTFCK"
     gene            1676..2704
                     /locus_tag="PKI33_03655"
     CDS             1676..2704
                     /locus_tag="PKI33_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36129.1"
                     /translation="MVRKIGTIVVFILAAVLLVYSATRSLDFISLTLPEDRKVLAYFG
                     LAALDGGLVAWILSYLYGSKGWQRAVSFLMVLVDLVGAIAMFTLDTLYNTGKAGMTEA
                     LTPSEMQTAVLGLSGVIALNIAAAVAHHLTDPEKLREQAEEDAFSKVEDATLKQISKN
                     ADSLAAQLAPTLAQDWMNQTRSRYMSYVGTGQIPTLIDSTAQNVPASSSAPVPFAMLQ
                     DNPKQTVSFMQFLPAWLTTKFGGGRKFEAAVPAPSVELKSHSEMFLYKCPSCGWEIKT
                     PEKFTNTKPCMNCDGFIELQTLKPQNNSLYEAFKQGIAEAQGVESADLHSPLGGSDAV
                     PTEGPEPK"
     gene            2986..3180
                     /locus_tag="PKI33_03660"
     CDS             2986..3180
                     /locus_tag="PKI33_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36130.1"
                     /translation="MKVEIYSIDTNETISENTHQSVADAEKFIALIKEYGFEDTNGNR
                     HKFSDAKVTPQGAVVYVEKE"
     gene            3501..>4428
                     /locus_tag="PKI33_03665"
     CDS             3501..>4428
                     /locus_tag="PKI33_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNM36131.1"
                     /translation="MTKSNAIITQASSEIQIDQNAAAIYIASLGSESGRRTMLQVLNL
                     IAGSISRNENAMTMDWSSLRFQHVAAVRSKMQEHYKPATVNKALAALKGTLRASWQSG
                     QMSAEDYQKAVSVKGVRGETIPTGRELSSGELAALLSSCEEDPSPAGVRDAAVIAVMY
                     SCGLRRAEVVTLDYADYDDESGRLAIRGKGKKERTAYITGGAALALNDWLSLRGSNPG
                     ALFVAVNKAGVLGDARITSQAIYNMLQKRASEAGVKPFSPHDMRRTFVSDLLDSGADI
                     ATVSKMAGHANVTTTARYDRRPEDAKRKAASLL"
BASE COUNT         1170 a         1035 c         1177 g         1046 t
ORIGIN      
        1 acaagtactt ttgcgacaat aaataatgta cccccgtcac gccactttca ttagttcatc
       61 aaagcgctca ttgccggagt cgtatggttg acctggtcgc gctggtggat cttcatcacg
      121 tggtgtttct acagaaacgg tgagaccggt ccggatatcg gcgccggcat cgatgaccag
      181 acataggcaa agtaaaagcc gcctcgtgag cacgaggcgg cttgttgttg gtggggcggt
      241 ggagtctatt tcttttttgg ctgctcttct tcgatgtatt caaacagatc accaggcttg
      301 acactgaagt actgacagag agcatcaatc acgggcacat caaagcgagt aacagtattg
      361 ttctcccagg cgtagagggt ctttcgagaa atgcctattt cgtctgcgac ttcagcaagg
      421 gagatgttgc ggcgctcttt tatgcgcttt tcggcgagaa ggatggcgaa gcgattattg
      481 acggtcacac tcatatttta ctccatttgt acactagtgt tacagatttt tgacccttga
      541 caggctgtta ccctagagtt acaatagcga aagtttagaa acagtaacta ttgtaacaca
      601 aaagtaagaa cggaaaacga aggagataag cgatgaatag ccagaaccgc ctcaccaaat
      661 ccgagatatt cacagtctac aaccagatgc gcgctgcggc tcgccgcgag ggaaatctca
      721 agttgattga acgcttgaac aaggctgtcg gcattttgct gagcaaggaa tactaccaag
      781 gtgaacgcct cgcttatatc ccgaccgctc attcctgtgg gtgccacgat tgggaattcc
      841 gcaacactgc caaacgtggt tacaccggtg gatgcaaaca catgcttgct gaagccatga
      901 tgcagtcggc tcttgcgctt cgcgctgctc atgaagtcac agtctggctt actccgcgag
      961 agtcgttccc tgcggaggtc gcagttcgat aacctagtgg ataccagcgc tactggcagc
     1021 gctgttccaa gcccgcctgt gtccttcgca tgggcgggca accttatctc agaagaggag
     1081 catccaatga gaccaaagcc aagaccaaga aaacgaaagc ccaggagagt cctgggagat
     1141 tggaggaagt tatttgacca aatgacaaaa ggggagaggc ttgaaatggc atgtctgatg
     1201 ttgaagacca tcgagagtcg ttcgcatgtc ggaatgaaaa agcccgctta ggaaatcggt
     1261 acataaccta agcgggcacc gtgggaagac ggtacgttta ttttaccctg aggaggtaac
     1321 cttggctaag aaaatttttc ctctgcttgt gttgttgtcg gcgattgctt acggtctcgt
     1381 ctggcttatg ccagacgttg aaccggaaaa cgcatcaccg atcgtaattc catcatcaca
     1441 gactctacag tcaccaatgc cggcaacgcc aggcgatttc tgcaaggtgg tgactggtct
     1501 cgaaacagga cgtgtcaacc tgcgcgaatg cgcagggacg gcgtgtcctg ttttgcgtgt
     1561 tctggaagat ggtcagatgt tgaccgtagt caaagccggt gcgtggaacg aagtcacaac
     1621 agtggacggc gttcgcggct ggataaattc aaccttttgt aaataggaga aatatatggt
     1681 tcgcaaaatc ggtacgatag ttgttttcat tttggcagcg gtgttgctgg tgtattctgc
     1741 gactcgcagt cttgacttca tcagtctcac gcttcctgag gaccgcaagg tccttgctta
     1801 ttttggtctc gctgcgttgg atggtggtct cgtcgcgtgg atcttgtcct acctgtatgg
     1861 ctccaaggga tggcagcgtg ctgtttcgtt tttgatggtg ttggtcgacc tggtcggcgc
     1921 tattgccatg ttcactttgg atactctcta caacactggt aaggcgggca tgactgaagc
     1981 tctcacacct tctgaaatgc agaccgcagt cctgggattg tccggagtca ttgctttgaa
     2041 catcgccgcg gctgttgctc atcacctgac ggatccggaa aagctgcgcg agcaagctga
     2101 agaagacgca ttcagcaaag ttgaagatgc gactctcaag cagatctcaa agaatgcaga
     2161 ctctctcgcg gctcagcttg ctccgactct tgcccaggac tggatgaacc agacacgctc
     2221 tcgatatatg tcctatgtcg gcactggtca aattccgacc ttgattgata gcaccgcgca
     2281 aaatgtgccg gcatcgagca gcgctcctgt gcccttcgcc atgttgcagg ataacccgaa
     2341 gcaaacagtc agcttcatgc agtttcttcc ggcgtggctc actacgaagt ttggaggtgg
     2401 tcgaaagttt gaagccgcag tgcctgcgcc atccgttgaa ttgaagtcgc acagtgaaat
     2461 gtttttatat aaatgccctt catgtggatg ggaaataaaa acccctgaaa aatttaccaa
     2521 tactaagcct tgcatgaatt gtgacggttt tattgaatta caaactttga agccacaaaa
     2581 caattcttta tacgaagcct tcaagcaagg cattgctgaa gcacaaggcg tagagtctgc
     2641 tgatcttcat tctccgcttg gtggaagtga cgcggtccct accgaaggac ccgaaccgaa
     2701 gtaggaataa gagtctgtgc tcgctgcggt ctcgactatg tcggcgcttt acatggacgt
     2761 tactgttcgc agtcctgcaa gcaaaaagca tatcgagacc gcaagcggca cggcggaacg
     2821 ggtcccaaaa aatcagatct ccggacttgt gctcgatgcg gaatacaatt cgaaggtgct
     2881 cttcatggtc gataccatag ccaggcatgc aaacaggcag cctacagaga gcgtaagcag
     2941 cgccagaacc aaatggcgac tttgaattga acaaaggaga taaccatgaa agtagaaatc
     3001 tatagcatcg atacgaacga aacaatttca gagaacactc atcagtccgt tgcagatgca
     3061 gagaaattta ttgctctcat caaagaatat ggctttgaag ataccaatgg caatcgccac
     3121 aaatttagtg atgccaaggt gaccccgcaa ggtgcagttg tgtacgttga gaaagaataa
     3181 ttatttggca agttgaaagc ctgaacctta acaacaaaat atcgtcatct cccagccgtg
     3241 ggaagcggac agactctcga agtgatttcg atggtgagta ctcaaaatgc gggaaggtga
     3301 cgaaacgaca atataataaa aaagccttgt agaatgcgtg agagtcgtct gggtggagaa
     3361 tatcccacag gacagacgga gcgagtcgct acgggcttcc tgacacgaaa gagggcatgt
     3421 ccagcaatgg acatgcccta aaaatgctgt ttttcagtta tgagaatggc acttctgaaa
     3481 ataaacgaaa ggagaacaga atgacgaaga gtaatgcaat cattacccag gcaagcagtg
     3541 aaattcagat cgaccaaaac gctgcagcaa tctacattgc aagtcttgga agtgaaagcg
     3601 gacggcggac catgctccaa gtgttgaacc tcattgccgg tagtatctct cgcaatgaga
     3661 acgctatgac tatggactgg tcatcccttc gctttcagca tgtcgcggct gttcgttcga
     3721 agatgcagga acattacaag cctgcgacgg tcaacaaagc tttggcagct ctgaaaggga
     3781 ctctgcgggc ttcatggcaa tcagggcaga tgagcgctga ggattatcaa aaggctgtga
     3841 gtgtgaaagg cgtccgtggc gagaccattc caacagggcg tgaattgtcg tccggagagt
     3901 tggcagcgtt gctttcttcc tgcgaagagg atccttcacc tgcaggtgtg agagatgcgg
     3961 cggttattgc agtcatgtat tcttgtggat tgcgtcgggc tgaggtagtc acacttgact
     4021 atgccgacta cgacgatgaa agcggacgcc tggcgatacg tggtaagggc aagaaagaac
     4081 gaacagcata catcaccggt ggtgcagctc tcgcgttgaa tgactggctg agtcttcgag
     4141 gaagtaaccc gggagcgttg tttgtcgcag taaataaagc gggagtcctg ggagatgcac
     4201 ggatcacttc tcaagcaata tacaacatgc ttcagaaacg agcgagtgag gcaggcgtaa
     4261 agcctttctc tccgcatgat atgagacgaa cctttgtcag tgacttgctc gactctggtg
     4321 ccgacatcgc tacagtctcg aagatggctg gtcatgccaa cgtaacaaca acggcacgct
     4381 atgaccgccg accagaggac gcgaagagaa aagctgcgtc cctcttgc
//
LOCUS       DAOREF010000123         4427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11488_length_4427_cov_4.539113, whole genome shotgun sequence.
ACCESSION   DAOREF010000123 DAOREF010000000
VERSION     DAOREF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11488_length_4427_cov_4.539113"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..704
                     /locus_tag="PKI33_03670"
     CDS             141..704
                     /locus_tag="PKI33_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNM36132.1"
                     /translation="MTTYCDYCNSHPEDTFNKTYHDTQYGFPIDDDNELFERLVLEIN
                     QAGLSWILILKRAQNFRKAFHKFNIKKVAKYTEADRERLLNDEGIIRNKLKVNATIVN
                     AQKILELQKEYGSFKGWLDAHHPLTKDEWTKLFKKTFVFTGGEIVNEFLMSTGYLPGA
                     HQKDCPIFKKAAAKKPAWMQPSKARKS"
     gene            710..1720
                     /locus_tag="PKI33_03675"
     CDS             710..1720
                     /locus_tag="PKI33_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNM36133.1"
                     /translation="MTSNFALAGKLTREYLLPLNGSPRLDSPGGNLLYAMSGLAVWDQ
                     SIALIARVNREYPQEWISDFQTRGLDVDGIYVDPESDRVDIRSFIAYTDTNERSITNA
                     VSHFARREMTFPKSLLGYHPQDPTIKDLRETDPLSPAARFVPKEYRNIGFVHLCPFDF
                     TSQSQLVNLFKGGSNQMVSLDPAPGYMKPKFWRELRVVLQGVTAFLPSEDELRSLFWG
                     ESHDLWEMAQRISEHGPQIIVIKRGPQGQLVYDAASKRRYEVPAYTARIMDPTGVGDA
                     FCGGFLAGYQRTNDPLQAALHGNVSASLKIEGVGVYATLDAMPGLADARLHALKEMAR
                     EI"
     gene            1763..2851
                     /locus_tag="PKI33_03680"
     CDS             1763..2851
                     /locus_tag="PKI33_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNM36134.1"
                     /translation="MTLAKKVLDLAIQIQQIASPTFAEGPRGEFIRDLFIQEKLDDVS
                     MDSLGNVYARLPGKQKKARALIVSAHLDTVFPAGTKLQIKEEAGKVFGPGIGDNSIGV
                     AALFGILWGIRERKIELKHDVWFVANVGEEGLGDLRGMRGVVERFGNDELGYLVLEGL
                     ALGHVYHRAIGVRRYRITAKTRGGHSWSDYGQPSAVHELAAMVTQLTQVRLPREPRTT
                     MNVGTIHGGTGVNVLAAEAKCELDLRSEDPVVLAKIVSQVEDIILHWNHEGVKIQAEI
                     IGERPAGQVASDHPLVKLAVESLNAQGMEAILTTGSTDANVPLSKGIPALVMGITTGG
                     GAHTMGEYIDTEPIERGMESLIRFVELS"
     gene            complement(2900..>4427)
                     /locus_tag="PKI33_03685"
     CDS             complement(2900..>4427)
                     /locus_tag="PKI33_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNM36135.1"
                     /translation="REHGNMLALVETGQVINSSLELDEVLRIVMDNIVRLTKAERGFL
                     MLRNEGGEMINRVARNWEMESINPSEKNVSRSVVQRVIDTGESVVTTNAQEDQRFVGQ
                     ESIVAFNLRSILCVPLKVKNDLIGVIYADNRIRAGIFAEAEKDLLEAFANQAAIAIDN
                     ARLFSSLKGTLEEVTALKNLMDNVFASIASGVITADVQDTITLANRAAETILGSPTQD
                     IIGHHLKDALASVSGELAPHLMEVRKTDKPIVDLEISHNLPKRGNVDWRLNLSPLKDA
                     GQKTQGVAIVLDDLTERKKLEAQRRLFERMVSPAVIEQLDPNGLQLGGKRTEITALFA
                     DIRGFTTYSEHLAPERLVSILNLYLAAMADAVLAQEGTVDKFMGDAIMAWFNAPIPQP
                     DHTLRAVKTALAIRESIENLYKELSSDSHLAFGVGIHFGDAVLGLIGTEKRLEYTAIS
                     DTVNTAKRLQENSARNQIIISQDAYERVKNDVNVKPISEITVKGRSKPIEVFEVLGLK
                     "
BASE COUNT         1148 a         1013 c         1100 g         1166 t
ORIGIN      
        1 aagcgttggc ggatcacaac ctttgtccgc aggggaggga tgcgcaactt cgtcgtcaca
       61 acagggattg gcatgtgggt aattataaag tgcctagctg gaatctccgc aagggtttag
      121 cgagatagta aaatatactc atgaccactt actgcgacta ttgcaattct caccctgaag
      181 acacgttcaa taaaacctac cacgatactc aatatggctt tcctattgat gatgataatg
      241 aactcttcga acggctcgtg cttgagatca accaggctgg actttcatgg attttgatcc
      301 tgaaacgagc acagaacttt cgcaaagcct ttcataaatt caatatcaag aaagtcgcca
      361 aatacactga ggcagaccgc gaaaggcttt tgaacgatga aggtattatt cgtaataagt
      421 tgaaggtcaa tgctacaatc gtcaatgcgc agaagattct tgaattgcaa aaagaatatg
      481 gttcctttaa aggctggtta gatgctcacc atccactcac caaagacgaa tggaccaagc
      541 tatttaagaa gacctttgtt ttcacaggtg gtgagatcgt caacgagttt cttatgtcca
      601 cgggctatct gcccggcgcg catcaaaaag actgcccaat cttcaaaaaa gcagccgcca
      661 aaaagccagc ttggatgcaa ccctccaaag ccagaaaatc ataacctgaa tgacatccaa
      721 cttcgcactg gcaggcaaac ttactcgcga ataccttctt ccgctgaatg gaagtccgcg
      781 tttggattca cccggcggga atcttttata tgccatgagc ggcttggctg tatgggatca
      841 atccattgca ttaattgcga gagtgaatcg agagtatccg caagaatgga taagtgattt
      901 tcaaactcga ggtctcgatg ttgatggtat ttatgtagac cctgaaagcg accgtgttga
      961 tatccgtagt tttattgcat ataccgatac caatgaacgc agcattacca atgccgtttc
     1021 acattttgct cgtcgcgaaa tgacctttcc caaatccctg cttgggtatc accctcaaga
     1081 cccaactata aaagacttac gcgagaccga tccactttct cctgcagcac gatttgttcc
     1141 gaaggaatat cgcaatatcg gctttgtaca tctttgtcct ttcgacttta ccagccagag
     1201 ccaattggtt aatcttttca agggcggctc aaatcagatg gttagtttag accctgcccc
     1261 cggttatatg aaaccaaaat tttggcgaga actacgcgtt gtgttgcaag gcgtcactgc
     1321 cttccttcca tctgaagatg aattgcgctc gctgttttgg ggagaaagcc atgacctttg
     1381 ggaaatggca caacgtatca gtgagcatgg acctcagatc attgttatca agcgtggacc
     1441 tcaaggtcag cttgtatatg atgccgcctc gaaaagacgt tatgaagtgc cagcctatac
     1501 tgcccgcatc atggacccga ccggagttgg tgacgctttc tgtggcggat tcctcgctgg
     1561 ataccagcgc acgaacgacc ccttgcaagc ggctttgcat ggaaatgtct ctgcttcact
     1621 aaaaatagaa ggggttggcg tttacgctac gctagatgcc atgcccggtt tggcagatgc
     1681 ccgtttgcat gccttgaaag aaatggcaag agaaatctaa caattgacga tttgtgactg
     1741 acgatttacg attgcagcca tcatgacact agccaaaaaa gtcctcgacc tcgctattca
     1801 aattcaacaa attgcatctc ctacctttgc ggaaggtccg cgtggcgagt ttatacgtga
     1861 cttgttcata caagagaaac ttgacgacgt gtccatggac tcgttgggga atgtctatgc
     1921 acggttgccg gggaaacaga aaaaggctcg ggcgttgatc gtatctgccc acttagacac
     1981 ggtgttcccg gcgggaacca agttgcagat taaggaagaa gcgggcaagg tttttggtcc
     2041 cggcattggg gataattcca tcggggtggc ggcgttgttc ggcatccttt ggggaattcg
     2101 cgaacgcaag attgagctga agcatgatgt gtggtttgtg gcgaatgtgg gggaggaggg
     2161 gctcggcgac ctgcgcggta tgcgtggcgt ggtggagcgc tttggcaacg atgaacttgg
     2221 ttatcttgtt ttggaaggct tggcgcttgg gcatgtgtat catcgcgcga tcggagtgag
     2281 gcgctatcgc atcacagcaa agacccgcgg cgggcattcc tggtcagact atggtcaacc
     2341 ctcggctgtt catgaactgg cggcgatggt cactcagtta actcaggttc gtttaccgcg
     2401 agaaccacgc actacaatga acgttggaac cattcacggc gggacaggtg tgaatgtgtt
     2461 ggctgcagaa gccaagtgcg aattagattt gcgctccgaa gacccggttg tattggcgaa
     2521 gatcgtctcg caagtggaag atattatttt gcattggaat catgaaggcg tgaagattca
     2581 agcagaaatt attggagaac gacctgcagg acaagttgcc tcagatcatc ctttggtcaa
     2641 gttagcggta gaaagtttaa atgcacaagg aatggaagct attctgacta caggctctac
     2701 agatgccaat gtcccattga gcaaaggaat tcccgccctt gtgatgggaa ttacaactgg
     2761 cggtggggca cacacgatgg gcgagtatat tgatacagaa ccgattgagc gaggaatgga
     2821 aagtttgatt cggtttgtag aattatctta agtgccatta tttcgaatca aaaaggcttc
     2881 catttggaag ccttttgttt tatttcaacc cgagcacttc aaaaacttct atcggttttg
     2941 aacgtccctt aaccgtgatc tctgagatcg gttttacgtt tacatcattt tttacacgct
     3001 catacgcatc ttgactaatg ataatttggt tgcgggcaga attttcttgt aagcgtttgg
     3061 cagtgttgac tgtgtcgctg atagctgtgt actcaaggcg cttttctgta ccaatcaaac
     3121 ccagaactgc atctccaaag tgaatgccta cgccgaaggc aagatgtgag tcactggaga
     3181 gttctttata caaattctca atcgattcgc gaatggcaag cgcagttttt accgcacgca
     3241 atgtatggtc tggctgtggg atgggtgcat tgaaccaagc cataatagca tcacccatga
     3301 acttgtctac ggtgccttct tgtgcgagaa cggcatctgc catggcagcg agatataaat
     3361 tgagaatgct caccaaacgt tctggtgcga ggtgttcact ataggtcgtg aacccacgaa
     3421 tatctgcaaa caaagcggtg atttcagtgc gtttaccgcc gagttgtaaa ccgttcgggt
     3481 caagttgttc gatgacggct ggagagacca tccgttcaaa caggcgacgt tgcgcctcta
     3541 acttctttcg ttctgtgagg tcgtccaaaa cgattgctac gccttgagtt ttctgtcccg
     3601 catcttttag cggagagaga ttcaagcgcc agtctacatt cccgcgtttg ggcaggttgt
     3661 gactgatttc aaggtcaacg ataggtttgt cggtcttgcg aacttccatc aaatgcggag
     3721 ccaattcacc tgaaaccgaa gcaagcgcat ctttaagatg atgaccgata atgtcttggg
     3781 tgggggagcc gaggatagtt tctgctgcac ggttggcaag agtgatcgta tcttgcacat
     3841 ctgctgtgat aacgccactg gcaatggaag caaagacgtt gtccattagg tttttcaaag
     3901 ccgtgacttc ttcaagtgta cctttgagag acgagaacaa gcgtgcattg tcaatggcaa
     3961 tggcggcttg gttggcaaac gcttccaaca aatctttttc agcttctgcg aaaatccctg
     4021 cacggattct gttatcggca taaataacgc cgatgaggtc attttttact ttaaggggta
     4081 cgcacaaaat cgagcgtaga ttgaaggcaa cgatactttc ctgcccaaca aagcgttgat
     4141 cttcttgtgc attggtagtg acaaccgact cgcccgtatc aatcacacgc tgaacaaccg
     4201 aacggctgac atttttttca gaagggttaa tggattccat ttcccagttg cgagccacgc
     4261 ggttgatcat ttccccgcct tcgtttcgca acatgagaaa accgcgctcc gctttggtga
     4321 ggcgcacaat attatccatt acgatgcgca gcacttcatc caactctaga gatgagttaa
     4381 tgacctgacc ggtttccaca agcgccaaca tattgccgtg ctcacgc
//
LOCUS       DAOREF010000124         4424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11507_length_4424_cov_4.293431, whole genome shotgun sequence.
ACCESSION   DAOREF010000124 DAOREF010000000
VERSION     DAOREF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11507_length_4424_cov_4.293431"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1229
                     /locus_tag="PKI33_03690"
     CDS             102..1229
                     /locus_tag="PKI33_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNM36136.1"
                     /translation="MTSAEIITIGTEILLGEIVDTNTRYIARTLRSLGVDLYRTVTIG
                     DNTDRIAEAIQRSMERANIIITTGGLGPTVDDPTREAVAQAVGVQTEFREDLWQQVVE
                     TIGRYGRTPSENQKRQAYVPQGALGLRNPVGTAPCFIVETETNAVISLPGVPAEMEHV
                     LHESVIPYLQKRFGLNEVIKVRVLHCAGLGEGMIDEKIADLEELSNPTVGLAAHTGVV
                     DVRITAKAKSESEANEMIAKIEADVRQRLGNVVFGADEDKLEEVTLDMLAKRGLTLTA
                     IESGLDGLLARKVPHTASKPDLDPAALMDALRAARADSKADIALGVSVYVNERAAEMA
                     MITAKGEKSHRITYGGPPKSLPRWSMNLALNWLRTTLEEMK"
     gene            1229..>1655
                     /locus_tag="PKI33_03695"
     CDS             1229..>1655
                     /locus_tag="PKI33_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36137.1"
                     /translation="MAKPKPRKKNDTPLVILAWGLNIFGLCALALVGVAYFALNPVSA
                     ATQAVSVNPAYTATMPPTYTPDVLSINLPTITPNPLSTMIVVQSPTPFVLQNAGTLGR
                     ASVATVGYSVSGRPIEVYMFGNGEHEKMIVAGIHGGYEWN"
     assembly_gap    1656..1697
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1698..2233
                     /locus_tag="PKI33_03700"
     CDS             <1698..2233
                     /locus_tag="PKI33_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNM36138.1"
                     /translation="DHPEVIPSDVTLYVLRNMNPDGDARDHGIDGRVNDHGVDLNRNF
                     PANWAKTWDRTGCWDLTKTTGGTGPGSEPETKAVMYFLQSHNVETLISYHSAALGVFP
                     GGIPWEADSKQFAKALAKATNYPYPPIDTGCVYTGTLADYAVELGVTAVDMELSTHKY
                     TDFDQNVKALEVLLNWR"
     gene            2286..4259
                     /locus_tag="PKI33_03705"
     CDS             2286..4259
                     /locus_tag="PKI33_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNM36139.1"
                     /translation="MTQTIDTLFINAIVLTMDEKLTQYDPGAVAVNGDSIVAVGAEAE
                     LKKEYSAKEVVDCGGKILMPGLVNAHTHVPMTLVRGLADDLRLDVWLMGYMLPVERQF
                     VSPEFVRLGTLIACAEQIRSGVTTFNDMYFYEDDIAQATADAGMRAVCGESVMKYPAP
                     DAASYDDSLAYAREFIKKWKGHPLIVPAIAPHAPYSTNPEIIRACVDLAKEFDVPLHI
                     HISETAFEVENMRKEQGMPVVPYVKKLGLFEAKVIAAHCVHIDTGEIRTLQHYKAGVS
                     HNPSSNLKLASGFAPVQKMLEDGLNVGIGTDGPASNNDLDMFEEVRLASFVAKAASND
                     PTVLPAATALTMATRLGAQALHIGHLTGSLEVGKRADLILVDTNPLHNTPRFKRDPQN
                     AYAQLVYASKSTDVTDVMVNGKWLMRDHQLLTLNEKELIAQARELATKIDAFLTEREQ
                     SVLSKLIALGGSMEEESFEVQVKVKVEEPNLIVQNVKRPEIEINAYKHYRQFDEYFLF
                     EDPNQGRLRYREDSLINEKGDIVNVRSRLTLLGVKREDEMGEVLLSRSRFLAPAAQSL
                     RFYREYFKPKSEISVEKNRFRWHIKYKNTEFFINLDEVKEPAMGHFLEVKSRTWSRKD
                     ADLKADLVRELLDILGVGNAETVTQDYIEILTA"
BASE COUNT         1126 a         1171 c         1072 g         1013 t
ORIGIN      
        1 cttgacctga aacgcttcga agaaggtcgt ttgataaacg agtacaatgt agtttgacct
       61 cacccctgcc cctctcctaa aagagagggg ggaaattgaa tatgacctca gcagaaatca
      121 ttactatcgg tacagaaata ttactcggcg aaatcgtaga tacgaacact cgctacatcg
      181 cgcgcacgct tcgcagcctg ggcgtggatt tatatcgcac cgtcaccatt ggcgataaca
      241 cagaccgtat tgccgaagcc attcaacgtt cgatggagcg cgctaacatt atcatcacca
      301 ctggcgggct gggtcccacc gtggatgacc ccacacgtga agccgttgca caagccgttg
      361 gcgtgcaaac tgaatttcgt gaagacctgt ggcagcaagt ggtcgagact attggtcgtt
      421 atgggcgcac accttctgag aatcaaaaac gccaggcata tgttcctcaa ggtgcgcttg
      481 gattgcgcaa cccggttgga acggcaccct gttttattgt tgaaacagaa accaatgccg
      541 tcatttcttt gcccggcgtg cccgccgaaa tggaacatgt cctgcatgag tcggtcattc
      601 cgtatttgca aaaacggttc gggttgaatg aagtgatcaa agtccgcgtg ttgcattgcg
      661 cgggtctggg cgagggcatg atcgatgaaa agatcgcaga cctagaagaa ttgagcaacc
      721 caaccgttgg gctggcggca cataccggcg tggtagatgt tcgcatcacg gcaaaagcca
      781 aaagcgagtc tgaggcgaat gaaatgatcg ctaaaattga agcggatgtg cgtcaacgac
      841 ttggcaatgt agtcttcggc gctgacgaag ataagctcga agaagtgaca ttggatatgc
      901 tcgccaaacg cggcttgacc ctgaccgcca tcgaaagcgg actcgacgga ttgctcgccc
      961 gcaaagtgcc gcacaccgcc tccaaaccgg accttgaccc tgccgctctt atggacgctt
     1021 tacgcgccgc tcgagcagac tcaaaagcag acattgcgct tggcgtttct gtttacgtca
     1081 atgaacgtgc tgctgagatg gcaatgatca ccgccaaagg ggaaaaatcc caccgcatca
     1141 cttacggcgg tccgcccaag agcctgccgc gttggagcat gaatttagca ttgaattggc
     1201 tgcgcacaac ccttgaggaa atgaaataat ggcaaagccc aagccacgca aaaagaatga
     1261 cacaccgttg gtcatccttg catggggctt gaacatcttt gggttatgtg cgcttgcatt
     1321 ggttggagtg gcatactttg cgttgaaccc ggtttcggct gccacacagg ctgtttctgt
     1381 taatccagcc tataccgcaa ccatgccacc cacctacacg ccagatgttc tctcgattaa
     1441 tttaccgacc attaccccaa accctctttc aacgatgatc gtggtgcaat ctcccactcc
     1501 cttcgtgctg caaaacgcag ggacgttagg gcgtgccagc gtcgccacgg tgggatattc
     1561 cgtttctggc agacccattg aagtgtatat gtttggcaat ggtgaacacg agaaaatgat
     1621 cgtagccggg attcacggcg gttacgaatg gaatannnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnntcg atcatccaga agtgattcca agcgatgtga ccctgtatgt
     1741 tctgcgcaac atgaatcccg atggtgacgc tcgcgaccac ggcattgacg ggcgtgtcaa
     1801 cgaccacggt gtggacttga accgtaactt tcccgccaat tgggcaaaaa cctgggatcg
     1861 caccggctgt tgggacctca ccaaaaccac cgggggaacg ggtccaggct cggagccgga
     1921 aaccaaagca gtgatgtact tcctgcaatc acacaatgtt gagacgctca tcagttatca
     1981 cagcgcggca ctgggtgtct tccccggcgg cattccatgg gaagctgatt caaaacagtt
     2041 tgccaaagcg cttgcaaaag cgaccaacta tccctacccc cccatcgaca cgggttgtgt
     2101 ttacacaggc accttggcgg attatgcagt agagcttggc gttacagcag tagatatgga
     2161 actttctact cacaagtaca ccgacttcga tcaaaatgta aaagctttag aagtgctctt
     2221 gaattggaga tagcttctat ttcatatcgc tcagggcgat aatcaaacta taaaaaggag
     2281 attgaatgac ccaaactata gataccctct ttatcaatgc catcgtgttg acgatggacg
     2341 aaaaactcac tcaatatgac cccggcgcag tagctgtaaa cggcgattcc atcgttgcgg
     2401 ttggtgctga ggctgaacta aaaaaagaat attctgcgaa agaagttgtt gactgcggtg
     2461 gcaagattct catgcccggt ctggtcaacg cgcataccca tgtacccatg accctcgtcc
     2521 gcgggcttgc agacgaccta cgcctcgacg tctggctaat gggttatatg ctgcccgttg
     2581 aacgtcaatt cgtttcgcca gagttcgtac gccttggaac cctcattgcc tgtgccgaac
     2641 aaatccgcag cggtgtaact accttcaacg atatgtactt ctacgaagac gatatcgccc
     2701 aagccactgc cgatgcaggc atgcgcgcag tatgtggcga atcggttatg aaataccccg
     2761 cgcctgatgc cgcttcttac gatgactcgc ttgcgtatgc acgtgaattc atcaaaaaat
     2821 ggaaagggca tccgcttatt gttccggcta ttgcgccaca tgccccctac tccaccaacc
     2881 ctgaaattat ccgtgcctgt gtagacctcg ccaaagagtt cgatgtgccg ttgcacattc
     2941 acatttctga aaccgccttc gaagtggaaa acatgcgcaa agaacaaggc atgcctgtgg
     3001 ttccgtatgt caaaaagctt ggcttattcg aagccaaagt cattgccgcc cactgtgtcc
     3061 acattgatac tggtgagatc cgcactctgc aacactacaa agccggtgtt tctcataacc
     3121 catcttcaaa tctcaaactt gcctccggct ttgctcccgt tcaaaaaatg ctcgaagatg
     3181 ggctcaacgt aggcatcggt acggatggtc ccgcttcaaa caatgacctc gacatgttcg
     3241 aagaagtgcg cctcgcctct ttcgtcgcca aggctgcttc aaacgaccca accgttctcc
     3301 ccgccgccac cgcgttgacc atggcaactc gcctgggggc tcaggcattg cacatcggtc
     3361 accttaccgg ctcgttggaa gttggcaaac gagcagacct gattctcgtg gataccaatc
     3421 cgctgcacaa cacaccgcgc ttcaagcgtg acccacagaa tgcctatgcc cagttggtat
     3481 acgcttctaa atcgacagac gttaccgatg tgatggtcaa cggcaagtgg ctgatgcgcg
     3541 atcatcaatt gcttacgttg aacgaaaaag aacttattgc tcaagctcgc gaactcgcaa
     3601 caaaaatcga cgccttcttg accgaacgcg aacagtctgt gctttcgaag ctcattgccc
     3661 tcggcggttc gatggaagaa gaatcattcg aggtgcaggt taaggtcaag gtcgaagagc
     3721 ccaatctcat cgtccagaat gtgaagcgcc ctgagatcga aatcaacgcc tacaagcatt
     3781 atcgtcaatt cgatgaatac ttcctgttcg aagatcctaa tcagggacga ttgcgttatc
     3841 gcgaagacag tctcatcaat gaaaaaggtg acatcgtcaa tgtgcgttca cgtttgactc
     3901 tcttgggtgt aaagcgcgaa gatgaaatgg gagaggtgtt gctctcgcgc agccgcttcc
     3961 ttgcgcctgc cgcacagtcg ctgcgttttt atcgtgaata cttcaagccc aagtctgaga
     4021 tttccgttga gaagaaccgc ttccgctggc acatcaagta taagaacacc gagttcttta
     4081 tcaacctcga tgaagtgaaa gaacccgcca tgggacactt ccttgaagtg aaaagccgca
     4141 cctggagccg aaaagacgca gacctcaaag cagacctcgt gcgagaactg cttgatattc
     4201 ttggcgttgg caatgctgaa accgtcaccc aagattacat tgaaattttg accgcgtaat
     4261 caataccaag ggcgacctgc ctgccatggt gtttatggca tcgggtcgcc cttatgaatt
     4321 taattaacta tggaaccctt tagaaaacca atagcaacat tctttgccac atcgttcgtt
     4381 gtcacagccg tgatggcgat cttattcttg aactttgacc gccg
//
LOCUS       DAOREF010000125         4397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11630_length_4397_cov_4.525104, whole genome shotgun sequence.
ACCESSION   DAOREF010000125 DAOREF010000000
VERSION     DAOREF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11630_length_4397_cov_4.525104"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PKI33_03710"
     CDS             complement(<1..925)
                     /locus_tag="PKI33_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM36140.1"
                     /translation="MPTTDMLKRDVIIIGGGPVGLSCAYYLLKSGRQVTLLDAKEIGK
                     GSGSGNAGHIVPSHIIPLAAPGVVTSALKWMLDPAHSPFGMKVSLNPNYLMWLLRFTI
                     ACSQNNVDRSLEPLNQLGQLSAKSFAQIIAEEKFDCAYQEMGLLHLFKTEKAFHEGQH
                     EGEFMQRHGIPVSVYDKNKIHEVEPAAVDDVLGGVHFTGDAHLNPAVFLNLLKERVKS
                     MGVELMDGTQVTGFETANGKITKVKTSAGDFEAEQIVLAAGALTPSVAKDLKLNIPVQ
                     PARGYSMTMSATKTMPSHALILGERRIAVSPM"
     gene            1134..1538
                     /locus_tag="PKI33_03715"
     CDS             1134..1538
                     /locus_tag="PKI33_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36141.1"
                     /translation="MKKFNIKSLLVFVVALIMVLSLSACATEEVSDASATDEAATEVA
                     TEVATEVATEEAGVIVPEFTFNNTGEEEICEIYLSPVGQDQWGPDQLGGATIPSGGSY
                     VLQNIPAGQYDVKAVGCAGSEVIGQLDIQPQQ"
     gene            1797..3191
                     /locus_tag="PKI33_03720"
     CDS             1797..3191
                     /locus_tag="PKI33_03720"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNM36142.1"
                     /translation="MQWQTNPYFAPIIISSLIAIVCAFFVITRRNRASGSTALLGLIF
                     SVIIWGFAYAMELASVSLKWQIFWAKVEYLGIPFVPLFFFIFAYQFTQSTSKLSRRVI
                     FFLVVISSIFVLLAWTNEFHNLIWTELGQKNIDGYYMLTLGHGMAFWLLITYSYILLA
                     IASISIIRRALSGPAEIRKQTYIILVGASVTWLGNIIYISGLSPVPELDLTPIGFVIS
                     SVIFSFGIFGYGMLDIMPIAGEFILESLDDVVIVTNPKGMLIYVNRAFEYYFSNPVQS
                     LIGRQATEAFSNLPVLKSLFDSSNTIRREITVTPENRPLFIFNVRTFNIRLSANEVIG
                     RVTILNDITERRAAENRLSQQSLEAGSGDLPLMVMYRVSDDKIVDVNRTFLINLGYER
                     KDVLGRTLMGVQFWGVNQRADFLRMLYKNGSLKDYAFEIGKNGQKYILSASQIEMQDT
                     KYIVIFAQEDWSGS"
     gene            complement(3264..3962)
                     /locus_tag="PKI33_03725"
     CDS             complement(3264..3962)
                     /locus_tag="PKI33_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36143.1"
                     /translation="MSINLGFYDFFSYLLPGFVYLYAVNDLLKVLGLKHVDLANFMQA
                     GQEPAFGLVTLALFAAYIVGHIFDPLSLKFFFQFVFRIRNQNGATTISLNTIKERYPQ
                     LNIKFAPKDWEALLIILRQRSFETAQNIDRFQADSIMLRNIAFGLLLMTLANVIAFFT
                     TMNVFYLLLAFAELFACWMLASESFKFRTWFFTQIYEGSLIYGTSIEEVISHGKGQVS
                     KSKGQKQTSTKIKL"
     gene            complement(3989..>4397)
                     /locus_tag="PKI33_03730"
     CDS             complement(3989..>4397)
                     /locus_tag="PKI33_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNM36144.1"
                     /translation="VNMQNLEALTDFSWRVREQLTANGITGANGAEIDHVELFASTPD
                     ADSKSFVLCPGKAYDRSPCGTGTSAKLACLYGDGKLQVGQIWKQQSVVGSIFEGSIQI
                     QDNRIIPTITGEAWVMSEGTILVDERDPFGKGI"
BASE COUNT         1153 a         1088 c          949 g         1207 t
ORIGIN      
        1 ccatcggtga gacagcgatg cggcgttcgc ccagaatcaa cgcatggctc ggcatggtct
       61 tggttgcgga catggtcata ctgtatccac gtgcaggctg aaccggaatg ttcaacttca
      121 aatctttggc aacggaagga gtcagcgcgc cagcggcaag cacgatctgt tctgcttcaa
      181 aatctccagc agacgttttg actttcgtaa ttttgccatt tgcagtttca aagcccgtga
      241 cctgcgtacc gtccatcaac tccacaccca tagatttgac acgttccttc agcaagttga
      301 gaaataccgc cgggtttagg tgcgcatctc ccgtgaaatg cacaccgcca aggacatcgt
      361 ccacggcggc gggctcgact tcatgaattt tgtttttgtc atatacactg acaggaatgc
      421 catgtctctg catgaattcg ccttcgtgct gaccctcatg gaaggctttc tcggttttga
      481 ataaatgcag tagtcccatc tcttgatagg cacaatcaaa tttttcttcg gcaatgatct
      541 gagcaaagct cttcgcgcta agttgaccaa gctgattcaa cggctccaat gagcgatcca
      601 cattgttctg gctacacgct atcgtaaaac gcaatagcca catcaagtaa tttgggttga
      661 ggctaacttt catcccaaag ggactatgcg ccgggtcaag catccattta agggcagagg
      721 tcaccacacc gggtgcggcg agcggaatga tgtgactcgg tacgatatgc ccggcattgc
      781 ctgaaccgct gcctttgcca atctcttttg catccagcag agtcacttgc ctgcccgatt
      841 tcaaaaggta atacgcacaa cttaacccca cgggtccccc accgatgata atcacatcgc
      901 gtttcaacat atctgttgta ggcatcgtta tcttctccaa agatcattca agtttgaata
      961 tttcaagtca ttatattcca gagccgcttg agtctatgtt gaccgaatcc aaatatgaga
     1021 accattaaaa agattccaaa ttaataattt tataatcaaa aataggttgc acttttttct
     1081 tttttatgta taatccgcgc ccgcacgtaa tctaaacaac ataaaggaat acaatgaaga
     1141 agttcaacat caagtctttg ctcgtgtttg tcgtcgccct catcatggtt ctcagccttt
     1201 ctgcatgcgc aactgaagaa gtcagcgatg cttccgctac tgatgaagct gccaccgaag
     1261 ttgccactga agtggctacc gaggtcgcta ctgaagaagc aggtgtgatc gttcctgaat
     1321 tcacctttaa taacactggc gaagaagaaa tctgcgagat ctacctctcc cctgtcggtc
     1381 aggatcaatg gggtcccgat cagctcggtg gcgccaccat cccctccggt ggttcctatg
     1441 ttctccagaa catccccgct ggtcagtatg atgtcaaagc cgttggctgc gctggctccg
     1501 aagtgatcgg tcagctcgac attcaaccgc aacaataatt gaatgacaga aaagacctct
     1561 gagtttttct cagaggtctt tttatttatg acatatccgc tctaaattca ctcgctatat
     1621 gccattataa tgagcggcgc tctaaaacat gggacgggtt gtaaaggttt cttgcaattg
     1681 cataaccctg tcttgacaaa cccacgaacc catgaataat tcactgaatt tctttgaaac
     1741 atttcagcca aagggtgttt ctaaaattaa cttttcaaag gtctgaaaac gttccaatgc
     1801 aatggcaaac taatccatat tttgctccaa ttatcatttc cagcctgatc gccatcgtct
     1861 gtgcattttt tgtcatcacg cgcagaaacc gggcatctgg ctcgacagcg ctcctcgggt
     1921 tgatcttttc cgtcatcatt tggggatttg cctacgccat ggagcttgcc agtgtcagcc
     1981 tgaaatggca gatattctgg gcaaaagtgg aataccttgg cattcctttt gtgcctttat
     2041 tctttttcat atttgcttat caattcacgc aaagcaccag caaattatcg cgccgcgtta
     2101 ttttctttct ggtcgttatt tcctccatat tcgtccttct ggcatggact aatgaattcc
     2161 acaatttgat ctggaccgaa ctcggtcaaa aaaatatcga tggctattac atgctgacgc
     2221 ttgggcatgg catggcgttc tggcttctaa tcacctactc gtatattcta ttggctattg
     2281 cttcgatcag catcattcgt agagccttaa gcggacccgc tgaaattagg aagcaaacct
     2341 atatcatttt ggtcggcgca agcgtcacat ggttggggaa tattatttac atttccggtt
     2401 tgagcccggt gcccgaatta gacttaaccc cgatcggctt cgtcatctcc agtgtcatct
     2461 tcagcttcgg tatctttggg tacggcatgc tagacattat gcccatcgcc ggagagttca
     2521 ttctcgaaag cttggatgat gtagttatcg tcaccaaccc caagggaatg ttgatctatg
     2581 tcaaccgtgc ctttgaatat tacttcagca accccgtaca atccctgatc ggcaggcaag
     2641 ctaccgaagc tttttccaac ttacccgttc tgaaatctct atttgatagc tcaaatacca
     2701 ttcgcagaga aattacggtc acgcctgaaa atcgtccgct gttcatcttc aatgtgcgga
     2761 cgtttaacat ccgcctcagc gccaacgagg tgattgggcg agtaaccatt ttgaacgata
     2821 ttaccgaacg ccgcgccgca gaaaaccggc tttcacaaca aagcctggaa gcagggtctg
     2881 gggatttgcc cttgatggtg atgtatcgcg tcagtgacga taagatcgta gatgtaaacc
     2941 gcaccttctt aatcaacctc gggtacgaac gcaaagatgt ccttgggcgc accttgatgg
     3001 gcgttcaatt ctggggcgta aaccaacgag ccgacttttt acgcatgctg tataagaacg
     3061 gctctctcaa agattatgca tttgagatcg ggaagaacgg acaaaaatat atcctttctg
     3121 ccagccagat cgaaatgcaa gatacgaagt acattgtgat cttcgctcag gaagattggt
     3181 ctgggtcgta atttcccact taaagaccaa gaggcaattt ggtgatacca aattgcctct
     3241 tttttattcc tttatccaat aaactacagc tttattttgg tgctggtttg tttttgaccc
     3301 ttactttttg aaacttgccc tttcccatgc gagatgacct cttcgatact cgtgccatag
     3361 atcaaagaac cttcatagat ttgagtaaag aaccaagtgc ggaacttaaa gctctcagaa
     3421 gccaacatcc agcaagcaaa gagctcagca aaagcaagca gcaaataaaa tacgttcatg
     3481 gttgtaaaga aagcgatcac gttcgcaagc gtcattagca aaagcccaaa ggcaatgttc
     3541 ctcaacataa tgctgtcggc ttgaaaacgg tcaatgtttt gtgcggtttc aaaactgcgt
     3601 tgacgaagaa tgatcaacag tgcttcccaa tcttttggcg caaacttaat attaagttgg
     3661 ggatatcttt ctttgatcgt gttcaaactg atggtcgtag ccccattctg gttgcgtatt
     3721 cgaaaaacga actgaaagaa aaatttgagg gagagtgggt caaagatatg ccccacaata
     3781 taggcggcaa acaaagccag agtgaccaag ccaaatgctg gttcctgccc cgcttgcata
     3841 aaattcgcca gatcaacatg cttcaagcct aatactttta atagatcatt gacagcataa
     3901 agatagacaa accccggcag taaatacgag aaaaaatcat aaaagcctaa attgatggac
     3961 attctatttt cctctcatct tggagtagtt aaatgccttt gccgaacgga tcgcgctcat
     4021 ccaccaagat cgtcccttcg gacatgaccc acgcttcgcc cgtgatcgtt gggatgattc
     4081 ggttatcctg aatctggatg ctaccctcga agatacttcc aacgacgctt tgctgtttcc
     4141 agatttgccc cacttgtagt ttgccatctc catacaaaca agcgagtttt gcactggtgc
     4201 ccgtgccgca gggagaacgg tcgtaggctt tgcctggaca caagacaaaa cttttgctgt
     4261 cagcatcagg tgtggatgcg aataactcca catggtcaat ctctgcgcca ttcgcgccgg
     4321 tgatgccgtt agcagtgagc tgctcgcgga ctcgccacga gaaatcggtc agcgcttcga
     4381 ggttttgcat gttcacg
//
LOCUS       DAOREF010000126         4395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11638_length_4395_cov_4.678341, whole genome shotgun sequence.
ACCESSION   DAOREF010000126 DAOREF010000000
VERSION     DAOREF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11638_length_4395_cov_4.678341"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKI33_03735"
     CDS             complement(<1..506)
                     /locus_tag="PKI33_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36145.1"
                     /translation="MLKIILHAPNPIMPFCEPARELRIQNTPLWLHQRNLLAPYVTRE
                     MELKQGERLQPVREPAIVYRDNLFFDEAFITEFMTQALKLNRPVRAAFRADDPAFREH
                     ALPLSTSYTPAGSLYLADLWYYPNGPVAGAEPLVIDMQAKEIGYYHVPTYMADQSGDL
                     VFQVPLRC"
     gene            complement(575..1186)
                     /locus_tag="PKI33_03740"
     CDS             complement(575..1186)
                     /locus_tag="PKI33_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNM36146.1"
                     /translation="MSETYHYPQKMGRIVLLGMEEVIGKGGMDAVLNFAALNDLQPSA
                     TNERTFSFETVSKLHQSLEQVYGPRGGRGLALRSGRACFKYGIKEYGSLLGLTEMAFR
                     LLPLPTKLHTGARSFADLFNKHTDQQVRVEETESQILWHIERCPLCWERKTEEPVCHL
                     AVGLLQEALYWLSGGKVFSVEETACHARGDEHCIITIQKTPLT"
     gene            complement(1198..1980)
                     /locus_tag="PKI33_03745"
     CDS             complement(1198..1980)
                     /locus_tag="PKI33_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM36147.1"
                     /translation="MSSITTDQGIVHYEVFGRGKPVILLHGYQGSWGLWQETMAYLGA
                     FYRTYALDFWGFGESGTKRSTYAVKDFVSLVYQFMEQLGIVRAPLVGHSMGGTVSLMT
                     AMQHPDRIQKVVVIGSPISGSSLYFFPRVFGYKTFGWLTYHNLWIYKGFYRILSPFYS
                     KDKNWAKMMDRDVSQVSLEAFFSSIGSLRRTDLRPSLHTIQAPVMGMYGDKDIVVNPN
                     QWKPLLDGVSKARIERFHTAGHFIMLDEPITFNQKLKSFLDE"
     gene            complement(2001..2537)
                     /locus_tag="PKI33_03750"
     CDS             complement(2001..2537)
                     /locus_tag="PKI33_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNM36148.1"
                     /translation="MQTVKMVVTGPFSAGKTQFIQSVSEIDVVATERKISSDEERSVK
                     SATTVAMDFGRITVDQDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFR
                     EARSILETFRAYAPTPFVVAANKQDKEDAWDLEDMGHALRLDKNTKLLPCVATDRNSV
                     KTILLELLYSIMSEMEGK"
     gene            complement(2565..3371)
                     /locus_tag="PKI33_03755"
     CDS             complement(2565..3371)
                     /locus_tag="PKI33_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNM36149.1"
                     /translation="MALRGNLRDFTITQLLNLINLASKTGTLVIDGASEQAHISFREG
                     KLAYARIGKEDGSLALVLYKANKINANQYKAIIDRAGQMSDKELGLLLINAGYVSRDD
                     ILLNLQAYFTDMVRRMFTWVEGVFRFEAEMLPPEDRINVRLDLENIIIEGSRQLRELE
                     QLQDEIPSLDMALKFTDRPLTNVNLSVDEWKVVKFVDPKNTMRQIAKTNKLTEVEVRR
                     IVYGLLQAGLVELVRPADAPMPQAAKPLAPQEVEDKKNLVNKLIGRIRSL"
     gene            complement(3499..>4395)
                     /locus_tag="PKI33_03760"
     CDS             complement(3499..>4395)
                     /locus_tag="PKI33_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36150.1"
                     /translation="TDEVGEEKANPLFMRAARAGLVASLIGMGATMLIGANNAAMPII
                     VGAIGVILIGIVLIFIMPETGFKPTPMENRSTWQHMWHTFNEGVKSVKARPRLMNILG
                     VGFFYGLYSEGFDRLWVKHLLDTFQIPVLFGGNQVAFFSLLRFSAGLLTVFVMQLIEK
                     RVDASSPLSIGRAMIIVTGVIAVGIVGFALSPLLPLTLGLYLTIYILRQVRIPLQTAW
                     VNHKLDPQTRATVHSMTGQVDSIGQILGGGSAFIARYLSVVAAITTSGILLGPALGLV
                     RRANSQSTHEAEVDPVGELTGD"
BASE COUNT         1196 a         1173 c          986 g         1040 t
ORIGIN      
        1 atacaacgca acggcacctg aaaaaccagg tcaccgcttt ggtctgccat gtaggttggt
       61 acgtggtaat acccaatttc tttcgcttgc atgtcaatga caagcggctc tgcacccgct
      121 acaggaccat tcgggtaata ccaaaggtcc gcaagatata agctacccgc cggggtatag
      181 gatgtagaca aaggcaaagc atgttcacgg aaagccgggt catctgcgcg aaaagccgct
      241 cgaaccgggc ggttaagctt caatgcctgc gtcatgaact ccgtaataaa tgcctcatcg
      301 aagaataagt tatcacggta aacaatggca ggttcacgta ccggctggag ccgttcaccc
      361 tgctttaact ccatttcgcg ggtcacataa ggggcgagca aattccgctg atgaagccaa
      421 agaggagtat tttgaatccg aagttcacgc gccggttcgc aaaaaggcat gatcgggttg
      481 ggcgcatgaa gaataatctt aagcatgggc agaattataa atcatagcgt gcctctcggc
      541 acgctatgta agaaaacaaa attgttatgc aattttaggt taaaggggtc ttctgaatgg
      601 taataatgca gtgttcatcc ccccgagcgt gacaagctgt ctcttccacg ctaaaaacct
      661 tgcccccact cagccaatac aacgcctctt gaagcaaacc taccgccaaa tggcaaaccg
      721 gttcttcggt ctttctctcc cagcacagcg gacagcgctc aatatgccaa agaatctggg
      781 actcagtttc ttctacacgc acctgctggt cggtatgttt attgaaaagg tctgcaaaag
      841 aacgggcacc cgtatgcagt ttggtcggga gcggcaataa acggaacgcc atttcagtca
      901 gcccaagtag agagccgtat tctttaatcc catacttgaa acaagcacgc cccgaacgga
      961 gcgccaatcc acgcccacca cgcggaccat aaacctgctc cagcgattga tgcaacttgc
     1021 tcaccgtttc aaacgaaaac gtccgctcat tggtagcgga cggctgtaag tcattcaagg
     1081 ctgcaaagtt gagaacggca tccatcccgc ctttgccgat cacttcctcc ataccgagaa
     1141 ggacgatcct tcccatcttt tgggggtaat gataggtttc actcatgagt tttgtattta
     1201 ttcatccaga aagcttttta gtttttgatt gaatgtaatc ggctcatcca gcataatgaa
     1261 gtgacctgct gtatgaaagc gctcaatccg cgcttttgaa acaccatcta ataagggctt
     1321 ccactgattt gggtttacga caatgtcttt gtcaccatac atccccatca caggggcttg
     1381 aatggtatgc aagcttgggc gcaagtccgt acggcgtagg gagccaatgc ttgaaaagaa
     1441 tgcttccaag gaaacctgtg aaacgtcacg gtccatcatc tttgcccagt ttttatcttt
     1501 tgaatagaag ggagaaagga ttcgataaaa acctttatag atccaaaggt tatgataggt
     1561 cagccaacca aaggtcttgt aaccaaaaac acgcggaaag aaatacagag aagaccctga
     1621 gatgggtgaa ccgatcacca ctactttttg aatccggtct gggtgctgca ttgcggtcat
     1681 caagctgacc gtcccgccca tgctgtgccc caccaatggc gcacggacga tgcccaactg
     1741 ctccataaat tggtagacca aactcacaaa gtcttttacc gcataggtgg aacgcttggt
     1801 acctgattca ccgaaacccc aaaaatcaag agcataggtg cgataaaacg cgcccaagta
     1861 tgccatggtt tcctgccata acccccatga accctgatac ccatgcaaaa ggatgaccgg
     1921 tttcccccga ccgaaaacct cgtaatgaac gattccctga tcagtcgtaa ttgaagacac
     1981 agtaagacat cacccgccca ctattttcct tccatttcgc tcataatgct gtacaacagc
     2041 tcaagcaaaa tggtcttaac ggagttgcgg tctgtcgcaa cgcagggcag cagtttcgta
     2101 tttttatcga ggcgcaaggc atgccccata tcttccaaat cccaagcgtc ttccttatcc
     2161 tgcttatttg cagccacaac gaagggagta ggagcataag cgcggaaggt ttcgagaatc
     2221 gaacgcgctt cacggaaggt ctctgggcgt gtgctatcca ccatcacgat gaatcccaac
     2281 ataccttcag aaaggatctc ccacataaag tcgaaccgtt tctgaccggg ggtgccgaac
     2341 aggtacagga ccagatcctg gtcaactgtg atgcgcccga aatccatcgc cacggttgtg
     2401 gcagatttca ccgagcgttc ctcatctgag gaaatcttcc gttctgtggc aaccacatcg
     2461 atctcactga cagactgaat aaactgggtc ttgccagcgc tgaatggacc tgtgactacc
     2521 attttgaccg tttgcataat tcctatcctt tcggcctgaa aaaattataa cgaacgaatg
     2581 cggccgatca gtttgttgac caaattcttt ttgtcttcca cttcttgcgg agcgagcggt
     2641 tttgcagcct gcggcatggg cgcatcggca gggcgcacca attccacaag acccgcttgc
     2701 aataaaccgt aaacaatacg gcgtacttca acttccgtta acttattcgt cttcgcgatc
     2761 tggcgcatcg tattcttcgg gtcaacaaac ttgaccacct tccattcatc cacgctcaag
     2821 ttgacattgg tcaacgggcg gtcggtgaac ttcagcgcca tatccaggct tggaatttca
     2881 tcctgcaact gctccaattc gcgcagttga cgtgaacctt cgataataat attctcaagg
     2941 tcaagccgta cattgatgcg gtcttcaggg ggcaacattt ccgcctcaaa gcggaacacc
     3001 ccttccaccc atgtgaacat gcggcgcacc atgtctgtaa aataagcctg caaatttagc
     3061 agaatatcgt cacgagaaac gtaccctgca ttgatcaaca acaaccccaa ctctttatca
     3121 gacatctgcc cggcgcggtc gataatggct ttgtactgat tggcattgat cttatttgcc
     3181 ttataaagaa ccaaagcaag gcttccatct tccttgccaa tgcgggcata ggctaacttg
     3241 ccctctcgga acgaaatatg tgcctgttcc gacgcaccat caatcaccaa agttcccgtc
     3301 ttgctggcga gattgatgag gttcaaaagt tgagtgatcg taaaatctct taaattaccg
     3361 cgcagagcca taattcctca aattcccttc atagggagcg ctcgaaatta tacttggtaa
     3421 acagataaag cgtcaattaa acctgtttgc actcttgtat gggtaggatt tatcaaattt
     3481 cccccgctat tccgactttt aatcgcctgt caactcccct actggatcaa cctccgcctc
     3541 atgtgtggac tgtgagttgg ctctgcgaac caagccgagt gctggcccaa gcagaatgcc
     3601 tgaagttgta atggctgcca ctaccgagag atagcgcgca ataaacgccg acccgccgcc
     3661 cagaatttgt ccaatcgaat ctacttgtcc cgtcatcgag tggacggtgg cacgagtctg
     3721 cgggtcaagc ttatgattga cccaagcggt ttgcagagga atcctcacct gacgcaaaat
     3781 gtaaatggtg agatacaagc ccaacgtcaa aggaagaagc ggggagaggg caaatccgac
     3841 aatgcccacg gcaatgacac ctgtcacaat gatcatcgcc cttccaatcg aaagtggact
     3901 actcgcatct acgcgttttt ctatgagctg cattacgaag actgtcagca agcctgctga
     3961 aaatctcaac agcgaaaaga aagcaacttg attaccgcca aacaggactg ggatttgaaa
     4021 ggtatcgagc aagtgcttca cccaaagacg gtcgaagcct tcactataca agccatagaa
     4081 aaagcccacg cctaaaatat tcatcaaacg cgggcgcgcc ttcacagact tcacaccttc
     4141 attgaaggta tgccacatgt gttgccaggt gctgcgattc tccatgggag ttggtttgaa
     4201 gcccgtttca ggcatgatga agatcaaaac aatgccaatc agaatcacac caatggcgcc
     4261 aacgataatt ggcatagctg cattgtttgc accgataagc attgtggcgc ccatcccgat
     4321 caacgaagcg accaagcccg ctcgtgccgc ccgcataaaa agcggattgg ctttctcctc
     4381 ccccacttcg tcggt
//
LOCUS       DAOREF010000127         4393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11649_length_4393_cov_4.373675, whole genome shotgun sequence.
ACCESSION   DAOREF010000127 DAOREF010000000
VERSION     DAOREF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11649_length_4393_cov_4.373675"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..211
                     /locus_tag="PKI33_03765"
     tRNA            128..211
                     /locus_tag="PKI33_03765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:162..164,aa:Leu,seq:taa)
     gene            278..1429
                     /locus_tag="PKI33_03770"
     CDS             278..1429
                     /locus_tag="PKI33_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNM36151.1"
                     /translation="MFFTRQQLEENEDRSLAPYGMRSKDTKGRAYLDTEPEYRTTFQR
                     DRDRILHTTAFRRLEYKTQVFINFEGDHFRTRLTHTLEVAQIGRTLARALGGNEDLVE
                     AICLAHDLGHSPFGHSGETALARLMKDYGGFDHNKQSLRIVTELEQRYPEFPGLNLSW
                     EVREGMVKHESEYDISDARDFSPDLRGNLETQIANVADELAYTTHDLDDGLRSGMINP
                     QILEGVALWEILRETYNWHGSILSDMERHRMIRHLVGILVTDMVKGTDARLKESKVNS
                     ALDIQKLKHNVVGYTEEMQRRNRELKDLLYKKLYRHYRVVRMQVKAERIISDLFNAFR
                     AEPATLPDHVQFFIEKRGLERTICDYIAGMTDRYAIEEHQKMFNPNEKP"
     gene            1519..1983
                     /gene="greA"
                     /locus_tag="PKI33_03775"
     CDS             1519..1983
                     /gene="greA"
                     /locus_tag="PKI33_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNM36152.1"
                     /translation="MTQPNYLTPEGEAKLKAELEELKGPRREELAQRLRSAIQMGDLS
                     ENADYHKAKEDQGFLEGRIQEIEAILRNSVLIEKTASSDVVSIGSHVTIQEEDFDPET
                     YHIVGPTEADPRKGKISHESPIGVALMNKKVGQIAEAQAPGGKIKFKILKIE"
     gene            complement(2028..3026)
                     /locus_tag="PKI33_03780"
     CDS             complement(2028..3026)
                     /locus_tag="PKI33_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36153.1"
                     /translation="MDFVVAFLKKLPVVPIVLFVVGLALGLLIGWGIAPVEFKDATPY
                     YLRPDLREDYMRMTIDSFSINKSAALAADRWLGLGSTAQEAYLAIQNNPGYLPSALVQ
                     EFGQAVTNEINARGGVPADPSTGMSPTMRTALIGIGVILLLGVVAAGGMYLFRLFGKR
                     GSGEVTVVMQAAEISRAAAKTNFEDMGLAPPITQTMTTYVLGDDLYDESFSIDTAAGE
                     FLGEYGVGVSEIIGVGEPKKVTALEIWLFDKNDIKTATKVLMSRHAFADPGIRSRLEP
                     KGELVLVEPQAQVLLETATLQLLATIVDIEYGKGPLPDSSYFERVTLELAIWPRQG"
     gene            complement(3179..>4393)
                     /locus_tag="PKI33_03785"
     CDS             complement(3179..>4393)
                     /locus_tag="PKI33_03785"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNM36154.1"
                     /translation="RTDSTNVSAIAQSETREFVTGKYGGDFLPAEPPTYKTKSANAQE
                     AHEAIRPTSVMRDPEKVKEFLEPAMYKLYRLIWQRFVASQMEAAVYDTLQVEVTGKTN
                     EHEYLLRASGSAIKFPGFMVVYEEAKNEDAKVEEDEEDVKIPAGVAEGQKQELVRLIP
                     EQHFTQPPPRFSEASLVQALEENGIGRPSTYAPTISTIQQRGYALREEKRLVPTDMGI
                     QVNDLMAQYFPEVVDLNFTAHMEEDLDKIADGQMEWVDAIREFYGPFAEDVKKAQAEM
                     PVAKSEPEPIGRKCPDDGGELVIRYGRFGKFISCANFPTCRYTEPWLEKIGVSCPKDG
                     GDMVERRTRKGRTFYGCANYPNCDFTTWKKPLAKPCPKCNGLLVIANKREAQCTNCSE
                     SFLLEEIVPENA"
BASE COUNT         1209 a         1125 c         1000 g         1059 t
ORIGIN      
        1 aacatgcggc cctgtcaagg ccgagatcgc gggttcgaat cccgtcggga ccgccaaata
       61 aatcgccttc actggcgatt tattttttgt ggcataaaaa gcaagttata gtaaaatatc
      121 ttctgccggg gcgatggcgg aatcggcaga cgcaacagac ttaaaatctg tcgttggaaa
      181 caacgtgagg gttcgacccc ctctcgcccc actctccact gtcattgcaa cactcaacac
      241 agattgttgc aatgacattt gcatctaagg acagaccatg ttctttacac gccaacaatt
      301 ggaagaaaac gaagatcgca gccttgctcc ctatgggatg cgtagtaaag ataccaaggg
      361 acgtgcttat ctcgacacag agccggaata tcgtacgaca ttccagcgcg atcgagatcg
      421 tattctgcat acaacagcat ttcgccgcct tgaatacaag actcaggtct ttatcaattt
      481 cgaaggggat cacttccgca cccgcctgac ccatacactt gaagttgccc aaattgggcg
      541 gacgcttgcg cgtgcgcttg gtggaaacga agacttggtt gaagccattt gtttggcaca
      601 cgaccttggg cattctccct tcggccactc tggcgagacg gcgcttgcgc ggttgatgaa
      661 agactacggt ggctttgacc acaataagca atcgttacgc attgtgaccg aactggaaca
      721 acgttaccca gaattccccg gtttgaatct cagttgggaa gtgcgtgaag gtatggtgaa
      781 gcacgagtca gaatatgata tttctgatgc gcgtgacttc agcccagatt tacgtggaaa
      841 cctcgaaacc caaatcgcta atgtggcaga tgaactcgct tacaccaccc acgaccttga
      901 tgatggtcta cgctccggga tgatcaaccc acaaattttg gaaggtgtag cgctttggga
      961 aatcttgcga gagacgtata actggcacgg aagcattctc agtgatatgg aacgtcaccg
     1021 catgatacgt cacttggtag gaattctggt cacagatatg gtcaaaggca cagatgcccg
     1081 cttgaaagaa agtaaagtca attcagcgct tgatatccaa aagctgaaac ataacgtagt
     1141 tggctatact gaagaaatgc aaagacgtaa tcgcgagcta aaagatttgc tctataaaaa
     1201 gttgtatcgc cattatcgcg ttgtacgaat gcaagtcaaa gcagagcgga tcatatctga
     1261 ccttttcaat gcttttcgcg ccgagcccgc cacattgcca gaccatgtgc aattcttcat
     1321 cgaaaaacgc ggactggaac gcactatttg tgattacatt gccggcatga cagatcgtta
     1381 cgccatcgaa gaacatcaaa aaatgttcaa ccccaacgag aaaccttaat ttctttcgtt
     1441 tctaaagggg gcatagagat agagttcgta tcaagcccct tatcgtctta tccaacgcac
     1501 atcaggaaag gagaaaccat gacacaaccc aattacctga cccccgaggg tgaagccaaa
     1561 ctaaaggctg agctcgaaga actgaaggga cctcgccgcg aggaattggc acagcgcctg
     1621 cgttctgcca tccaaatggg agacctctcc gaaaatgccg attaccataa ggcaaaggaa
     1681 gatcaaggct tccttgaagg tcgcattcaa gagatcgaag ccattttgcg caactcagta
     1741 ctaattgaaa agacagcctc ctcagatgtt gtctccattg gctctcatgt gaccattcaa
     1801 gaagaagact tcgacccaga gacgtatcat atcgtcggtc ccaccgaagc agacccacgc
     1861 aaaggcaaaa tctctcacga atccccaatt ggggttgcgc tgatgaacaa aaaggtcggg
     1921 caaatcgctg aagcacaagc gcccggcggc aaaatcaagt tcaaaattct aaaaattgaa
     1981 taaacaaaaa aacgctccac gtatgtggag cgtttttttg tttatattta tccttggcgg
     2041 ggccaaattg ctaattcaag tgtcacacgc tcaaagtagc tgctatcagg caaaggtccc
     2101 ttgccatatt caatatccac aatggttgcg aggagttgca gggttgccgt ttcgagcaac
     2161 acctgagctt gaggttccac aagaaccaac tcacccttag gttcgaggcg cgagcgaatt
     2221 cctgggtctg caaaagcatg gcgagacatc agaactttgg tcgctgtttt gatgtcgttc
     2281 ttatcaaaaa gccaaatttc taacgcagta accttcttcg gctcacccac gccaataatt
     2341 tcagaaacac ccacgccgta ttcaccaagg aactcacctg cggctgtgtc aatactgaaa
     2401 gactcatcgt aaaggtcatc acccaagaca taagttgtca tggtctgcgt aatgggtgga
     2461 gccagaccca tatcttcaaa atttgtcttg gcggctgctc ggctaatctc agcggcttgc
     2521 atcactacgg tcacttcgcc agagcctcgt tttccaaaca agcggaagag atacatgccc
     2581 ccagcggcaa cgacacccag taacaaaatc acaccaatgc cgatcaacgc agtgcgcatg
     2641 gtaggagaca tacccgtaga ggggtcagca ggtacgccac cgcgtgcatt gatctcattc
     2701 gtcactgcct gaccaaactc ttgcaccaaa gcagaaggta aataaccggg gttgttttga
     2761 atcgctaaat aggcttcttg cgctgtgctg cctaacccca gccaacgatc ggctgctaat
     2821 gctgcgcttt tgttaatgct aaaagagtca atcgtcatgc gcatgtaatc ttcgcgcaaa
     2881 tcagggcgca aataatatgg ggttgcatcc ttaaattcta cgggcgcgat tccccaacca
     2941 atcaacagcc caagtgccaa ccctacaaca aaaaggacaa ttggcacaac aggcaacttc
     3001 tttaaaaatg caacaacaaa atccataatt ggctccttcg cacagatgct ttcgctcgat
     3061 tatacaacgt aaattagggg cactgtatga cagaatgatt gcccacatgg ataaaaaaac
     3121 gcatcagact cagccatact tgccactacg accaagcctg atgcgaacgc tgaaagtttt
     3181 atgcgttctc tggcacgatt tcttctaaca agaaactttc agaacaattc gtgcactgcg
     3241 cctcgcgttt gtttgctatc accaacaacc cattacattt cgggcaaggt ttggctaagg
     3301 gcttcttcca tgttgtgaaa tcacaattcg ggtaattcgc acaaccatag aaggtgcgtc
     3361 ccttgcgagt gcggcgttcc accatatcgc cgccatcttt cgggcaggaa acaccgatct
     3421 tctccaacca cggttctgta tagcgacagg ttgggaagtt tgcgcaggaa atgaacttgc
     3481 cgaagcgccc ataacggata actagctcac cgccatcatc cgggcatttt cgtccaatcg
     3541 gctccggctc agatttagca acgggcattt ctgcctgtgc ctttttcaca tcctcagcaa
     3601 acggaccgta gaactcacga atggcatcca cccattccat ttgtccatcc gcaattttat
     3661 cgaggtcttc ttccatatga gccgtaaagt tcaaatcgac tacttcaggg aagtattgtg
     3721 ccatcaagtc attgacctga atccccatat cggttggcac cagtcgtttt tcttcgcgca
     3781 aggcatatcc gcgctgctga atggttgaaa tagtcggcgc ataggtagac ggacgaccaa
     3841 tcccattctc ttccagggct tgtacaagcg atgcttctga aaagcgtgga ggaggttgag
     3901 taaagtgctg ttcaggaatt aagcggacca attcctgctt ttgtccttcc gccacaccgg
     3961 cgggaatctt tacatcttct tcgtcttctt caaccttggc atcttcattt ttggcttctt
     4021 cgtaaacaac cataaagcca gggaacttaa tagcagaccc agaagcacgc aagaggtatt
     4081 cgtgttcgtt tgtcttgccg gtcacttcaa cctgcaaagt gtcgtataca gcggcttcca
     4141 tctgagaggc aacaaagcgt tgccaaatca agcgatatag cttgtacatg gcgggttcaa
     4201 ggaattcctt gaccttttcc gggtcacgca tcacagatgt ggggcgaatg gcttcgtgag
     4261 cttcctgtgc attcgcagac ttggttttgt atgtaggcgg ttctgccgga agaaagtcgc
     4321 cgccatactt accagtgaca aactctcttg tttcgctttg agcaatagca gagacattag
     4381 tcgagtcggt acg
//
LOCUS       DAOREF010000128         4383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11690_length_4383_cov_3.150416, whole genome shotgun sequence.
ACCESSION   DAOREF010000128 DAOREF010000000
VERSION     DAOREF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11690_length_4383_cov_3.150416"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKI33_03790"
     CDS             <1..326
                     /locus_tag="PKI33_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36155.1"
                     /translation="ASGKDGSPSMKEVFSHWVRPSLPLALFLMVISGLSAAQEVFTPY
                     LTSFSREQQTIAIAMAQLAGNFNPGSIAAILAVFGIPFFIITFSVLAILQNTYLNKLE
                     ITNGA"
     gene            complement(378..659)
                     /locus_tag="PKI33_03795"
     CDS             complement(378..659)
                     /locus_tag="PKI33_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36156.1"
                     /translation="MIKLYREAQSPKADMIEAEFKELVLGYDLVVVEPAKAESLFEGR
                     VLPVITNNERIVSGDEIMPYIKELTRLMYDWQLFQSDACYIRDDGNGIC"
     gene            727..1089
                     /locus_tag="PKI33_03800"
     CDS             727..1089
                     /locus_tag="PKI33_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36157.1"
                     /translation="MLISKVIMYEHHKQPLAKKSVFAKRLARNGVLGSLLLLFSLFIG
                     MLGYHFLEGLSWLDSLLNASMILGGMGPVNPLQTNAGKVFASLYALYSGVVLLAAVGI
                     LAAPIFHRFLHRFHLAEE"
     gene            1093..1482
                     /locus_tag="PKI33_03805"
     CDS             1093..1482
                     /locus_tag="PKI33_03805"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HNM36158.1"
                     /translation="MTEYLPIHFYDESIEVLFEKPPVYEKAPHCPNGFIWNGQTYQVI
                     AALSEWTDFSRKGKMAKNMRPAHALTAATRGSLNVGRYYFRVEVNTAQIFDIYYDRAM
                     KSVDDRKGQWLMYRELEKTNGSIPAGQ"
     gene            1457..2179
                     /locus_tag="PKI33_03810"
     CDS             1457..2179
                     /locus_tag="PKI33_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNM36159.1"
                     /translation="MDQYPLDNNPNLTPPLSTPKPASWSANDTWLGLALLLIVVAGYL
                     FALSRMEQNQSTNILYVATFEFLLLIPIAVIFLWRKVSWKELGFNRFNANQLALGCGL
                     LVGVYMLVIINNLVMISLGVTTQADVISELLGEINAPYLFAFTTVIVAPITEEMFFRG
                     FLFKGLREKYGWVNALMFSSMIFAIFHGQLATLLPTFLLGALFSYLYQRTDSVFPGMF
                     LHFTVNAMGALVLLFANQTGAL"
     gene            complement(2218..3468)
                     /locus_tag="PKI33_03815"
     CDS             complement(2218..3468)
                     /locus_tag="PKI33_03815"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNM36160.1"
                     /translation="MNTKQKPHVLIIGAGFAGLETARGLAKAPVRITLIDKNNHHLFQ
                     PLLYQVAIAGLLPSQIAQPVRTIFRKQKNLAFQMGEVTDINLNEKFVKMNGSVIAYDY
                     LVIAAGARTNFFGFDTLEKHGLQLKDLETAVTTRNHLLSRFEGASHEADPEKRKAMLT
                     FVIVGGGPTGVETAGALAELITHVMRKDYPRLNLNEAQVILLEAGPHLIAPYPDELRQ
                     ATLRLLRDKKVDVRLNTKMQDFNGQRVTLGDGSVIESQTLIWTAGAKAAEVIDALPAE
                     KASMGRVRIQATLSLPQFPEAFVIGDASFLANGNGQPLPMLSTVAIQQGQAAAKNIRL
                     MMEGKQPKAFHYKDPGLLATVGRNAAVARIFGISFSGFIAWVIWVFLHIYRIIGFRNR
                     LLVMFNWAWDYFFYDNQVRLITRE"
     gene            complement(3669..>4383)
                     /gene="arsB"
                     /locus_tag="PKI33_03820"
     CDS             complement(3669..>4383)
                     /gene="arsB"
                     /locus_tag="PKI33_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNM36161.1"
                     /translation="GLARCIAMVIVWNELAKGDSEYAAGLVAFNSIFQVLFYSVYAYV
                     FITVLPTWFGMTGNIVDITIGEIAKSVFIYLGIPFLAGFFTRFFLVRAKGKDWYHKVF
                     VPKISPMTLIALLFTIVVMFSLKGDVIVQLPLDVVRIAIPLLIYFVVMFLVSFFMGKS
                     IGADYSKTTTLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLIEVPVMIGLVNV
                     AFFFQKRYFTSTVQHAPAR"
BASE COUNT         1062 a         1184 c         1016 g         1121 t
ORIGIN      
        1 aggcatctgg caaagacggc tcccccagca tgaaagaagt cttttcgcat tgggtacgcc
       61 cttccctgcc gctggcttta ttcttgatgg taatctccgg tctttccgct gcgcaagagg
      121 tattcacacc ctacctcacc agcttctcgc gtgaacaaca aacgatcgca attgccatgg
      181 cgcaactggc agggaacttc aacccaggca gcattgctgc cattcttgcc gtgtttggca
      241 tcccgttctt tatcatcacg tttagtgtgc tcgccattct ccaaaacacc tacttgaaca
      301 agcttgagat cacgaacgga gcatagccat aaaaaatacc ccgctgactt tatatcagcg
      361 gggtatttta tgctcaatca acagattcca ttcccatcat ctcggatata acaagcatca
      421 ctttggaaca actgccagtc atacatcagg cgggtcaact ctttgatgta gggcataatc
      481 tcgtccccac tcacgatacg ctcgttgttc gtgattaccg gcaaaacacg cccctcaaaa
      541 agagactctg ccttggcagg ctccaccaca accaaatcat agcccagcac cagctcctta
      601 aactccgctt cgatcatatc ggctttgggg ctttgtgcct ctcgatacag tttaatcatt
      661 ctttctctcc ttcgtcattc agacgatttt acccagtaat ttcttccaat ccccacatca
      721 actataatgc tcatatcaaa ggtgattatg tacgagcatc ataaacaacc gcttgccaag
      781 aaatctgtct ttgccaaacg cctggctcga aacggcgtgc ttgggtctct gttgctgctc
      841 ttctctttat tcatcggcat gctgggctat cacttcctcg aaggtctctc ctggttagac
      901 tcgctgctca atgcctcgat gattctcggc ggcatgggtc cggtcaaccc gctgcagacc
      961 aatgctggga aagttttcgc ctcgttatac gctctatatt ctggcgtcgt acttctggca
     1021 gccgttggca ttcttgcagc gccgatcttt catcgcttct tgcatcgctt ccacctcgca
     1081 gaagagtaaa ctatgaccga atatctgccc atccactttt atgatgaaag catcgaagtc
     1141 ctcttcgaga agccgcctgt gtatgagaaa gcgccacact gccccaatgg cttcatctgg
     1201 aacgggcaga cctatcaagt cattgccgcc ctctccgaat ggaccgactt ctcgcgcaaa
     1261 ggcaaaatgg cgaagaacat gcgccccgcc catgccctaa ccgccgccac tcgcggctcg
     1321 ttgaatgtcg gtcgctacta ctttcgcgtg gaagtgaaca ccgctcaaat cttcgacatc
     1381 tactacgacc gcgccatgaa aagcgtagac gaccgtaaag gtcaatggct gatgtataga
     1441 gaattggaaa aaacaaatgg atcaataccc gctggacaat aacccgaacc tcacgccgcc
     1501 tctttccacg cccaagcctg cctcttggtc tgcaaacgac acctggctcg ggctggctct
     1561 tctgctcatc gtcgttgccg ggtatttatt tgcactttct cgcatggaac aaaatcaaag
     1621 caccaacatc ttgtatgttg ccaccttcga attcctttta ttgattccca ttgccgttat
     1681 cttcttatgg cgcaaagtct catggaaaga gctgggcttc aaccgtttca atgccaatca
     1741 attggcattg ggttgcgggt tgttagttgg cgtgtatatg ctggtcatca tcaacaatct
     1801 cgtgatgatc tcactcggcg tcaccaccca agccgatgtc atctccgagc ttcttggcga
     1861 aattaacgcc ccgtatctct tcgcctttac caccgtcatt gtcgcgccta tcaccgaaga
     1921 gatgttcttc cgtggctttc tcttcaaagg cttgcgcgaa aaatatggct gggtcaatgc
     1981 cttgatgttc agctcgatga tctttgccat ttttcatggg caacttgcta ctttattgcc
     2041 tacttttctg ctcggcgcct tgttctcgta cctgtaccag cgtacagact cagtcttccc
     2101 gggcatgttt ctacatttca cggtcaacgc catgggggca ttggtcttgc tcttcgcaaa
     2161 tcaaaccggt gctttataag aatacatcct cagagaaaat ctctgaggat gtattcttta
     2221 ttcccgcgtg attagtcgta cttgattatc gtagaagaaa taatcccatg cccagttgaa
     2281 catcacaagc agacggttgc gaaacccgat aatgcgatag atgtgcagga agacccagat
     2341 cacccaggca atgaatccac tgaaggagat gccaaagatg cgggcgacgg cggcgttccg
     2401 tcccaccgtg gcgagcagac cggggtcttt gtagtggaac gctttgggtt gtttcccttc
     2461 catcatgaga cgaatattct tcgccgcggc ttgaccttgc tggatcgcca cggtcgaaag
     2521 catgggcaaa ggttgaccat ttccattggc gagaaaagaa gcatcaccga tcacaaaggc
     2581 ttcaggaaac tgaggcaggc tcaacgttgc ctggatgcga acgcgaccca tgctggcttt
     2641 ctcggctggt aacgcgtcaa tgacctcggc agctttcgcg cccgcagtcc aaattagcgt
     2701 ttgactttcg atcacagagc cgtctcccag cgtcactcgt tgaccgttga aatcctgcat
     2761 cttggtgttc aagcgcacat ccactttttt atctcgcaaa agccgcagcg ttgcctgacg
     2821 cagttcatcg ggatatggcg cgatcaaatg cggacctgcc tccagtaaga tgacttgagc
     2881 ctcgttcaaa ttcaaacgcg gataatcctt gcgcatcaca tgtgtgatga gctctgctaa
     2941 tgcgcctgcc gtttccacgc cggtgggacc tcccccaacg atgacgaagg tgagcatggc
     3001 tttgcgtttt tcggggtcgg cttcgtggct ggcaccctcg aaccttgaaa gcagatgatt
     3061 gcgtgtggtg acggcggttt caaggtcttt gagttgcaag ccgtgttttt cgagcgtatc
     3121 aaagccgaag aagtttgtgc gcgctcccgc cgcaatgaca agataatcat aggcgatcac
     3181 ggagccgttc atcttcacga acttttcgtt caaattgata tctgtcactt cgcccatttg
     3241 gaaggcgagg ttcttttgtt tgcggaatat cgtgcgcacg ggttgggcaa tttgcgatgg
     3301 caacaaacca gcaatcgcca cctgatacaa cagcggctga aagagatgat ggttgttttt
     3361 gtcgataagg gtgatgcgta caggcgcttt ggcgagtccg cgggcggttt cgagcccggc
     3421 aaagccagcg ccgataatta agacatgggg cttttgcttg gtgttcatgg tttcctctgt
     3481 gttggcaggt gacagtcact ttagaaatga ttatcacctt tgttttattt gtgaaattta
     3541 tcaccattat tataaccaaa actgtcttat ttgtaaatat tttcacaata ggatgttagt
     3601 actgccccac ttaacggaaa agcctcccgc ttcatttcaa cgagaggctt gatacgacca
     3661 gtgagttttt atctggctgg ggcgtgttgc acagtcgacg tgaagtaccg cttctggaag
     3721 aagaacgcca cattgaccaa cccgatcatc accggcacct cgatcaacgg accgatgacc
     3781 gctgcaaaag ccgcaccaga gttgatgcca aagactgcca cagcgaccgc aattgccagt
     3841 tcaaagttat tgctagctgc cgtgaatgaa agcgtggtcg tcttggaata atccgcgccg
     3901 atggatttgc ccataaagaa tgagacaagg aacatcacta cgaagtaaat caaaagcgga
     3961 atggcaatac gcaccacatc aagcggcagt tgaacaatga catccccctt gagagagaac
     4021 atcacaacta tagtgaagag caatgcgatg agggtcattg ggctgatctt cggcacaaag
     4081 accttgtgat accaatcctt gcccttcgca cgtacaagga agaagcgcgt gaagaaaccg
     4141 gcgaggaacg ggatgccgag gtaaatgaac acgctcttcg cgatctctcc gatggtgata
     4201 tccacaatat tgccagtcat gccgaaccaa gttggcaaga cggtaataaa gacataggca
     4261 tatacactgt agaaaagcac ttggaagatg ctattgaaag cgaccaatcc cgccgcgtat
     4321 tcgctatcgc ctttggcaag ttcgttccac acgatgacca tcgcgataca acgcgcgaga
     4381 ccg
//
LOCUS       DAOREF010000129         4382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11693_length_4382_cov_4.584932, whole genome shotgun sequence.
ACCESSION   DAOREF010000129 DAOREF010000000
VERSION     DAOREF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11693_length_4382_cov_4.584932"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKI33_03825"
     CDS             <1..610
                     /locus_tag="PKI33_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM36162.1"
                     /translation="PLTTLRGNLGLLRRNPAPEEQTDILNDMVDESDRLIRLVNDLLQ
                     LARADAGRSLVKESVEISPLLQEVVRQAQLLDPQRTIQLNAADPLQVRGDRDAIKQVL
                     IIGLDNALKHSSSQVEVICKKLDGRVEIQIQDHGDGIPPEKLEHVFDRFYRGEDSASV
                     PGFGLGLSIAKSLIERQDGTIQMNSQVGKGSVLKLRLMISSN"
     gene            complement(656..1249)
                     /locus_tag="PKI33_03830"
     CDS             complement(656..1249)
                     /locus_tag="PKI33_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNM36163.1"
                     /translation="MNLPEILLESSSRHKHLCPRQVLGARMALYAGELLGLELPREDK
                     RLLVTSETDGCAVDGVIAASHCRVGSRTLRILDFGKVAATFTDTHTETSLRIAPQREA
                     RTQADEYASHAKNHWEAMLEAYQIMPFDKLFHVQKVCLITPLSEVISKPGKKILCGIC
                     GEEVINGREILLNESTLCKSCAGESYYLANTPVFADR"
     gene            complement(1246..2304)
                     /locus_tag="PKI33_03835"
     CDS             complement(1246..2304)
                     /locus_tag="PKI33_03835"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNM36164.1"
                     /translation="MPRKKETPPPPPAQEEESVWTYRGYKLKSSEFVTAMVHLFRAEV
                     QRANVWRQRLDTTTNWAVVATGATLSISFSQPNVHHGVIILNTLLVTWFLFIEARRYR
                     YYELWSYRVRLMETDFYAPMLVPPFHPSPEWAENLAENLLTPSFPISMWEAFGRRLRR
                     NYLWIYLILYISWMGKSFLFPEPATTIAEFVGRGAVGSIPGFVMISLGLAYHLFLLII
                     AIGTVGMTKATGEVLPRFGDETAPAAPSMEGKHTGLRSLLVPRKRRRQLLALIITDKA
                     KEVSSRIMNDLKRGVTSMEGKGMYTGKDRSILMCALTITEAHALKSAVAKEDPKAVVI
                     VSPAQEILGGGFAPLEEK"
     gene            complement(2310..4109)
                     /locus_tag="PKI33_03840"
     CDS             complement(2310..4109)
                     /locus_tag="PKI33_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNM36165.1"
                     /translation="MSQITLTLIFLGIAAILLLRNILRADIVALMLMLALGLSGILTP
                     QEAFSGFSRQAVIIMLSAFVLAEGLRRSGMTERIGLFIIKLFGAGERRLIFGVMTASA
                     VLSLFMNNIAAASLLFPSLSGVARKMKISPSKLLMPLAFGTILGGMATLLTTVNLVVS
                     GLLRDAKLSAFSLTDFLPVGLPLAFAGIMFMVLWGRKLLPSQSPAQRHEEENASDELI
                     KTYQLAERLVRAEVCKGGSLDGVTLEKSGLREKHHLNVIAVQRGRNTLPIEAKMILKG
                     DDILLIMARPEDSVPESLGEMLEVLPSGEWEQDYLSTPNMKLIEAAIAPRSHLASQTL
                     SEIRFEQKFNSQVLAIWRRGRSIRTRLADLPLEFGDGFLMQGTEKSLSLLRTEPGLVL
                     LAESAPSTRMTWRNWLTVLIMFATLALAVLFSDNIAEIMLTGSVMMVLIGTMSMDQAY
                     RSIDWRSIFLVAGMLPLGVALNKTGASTLFADAILSAFGNASHLVLLASFVLLTVALT
                     QIINGAAAVTVIAPIAIATSQQVGMEPRSVAMAVALASSMAFMSPLGHAVNVMVMGAG
                     GYTFKDYAKVGIPLTILLVIILLIILPILMPIG"
BASE COUNT         1149 a         1169 c         1052 g         1012 t
ORIGIN      
        1 gccgctcacc accctgcgcg gaaatttagg cttgttacgc cgcaaccccg cccccgaaga
       61 acagacggac atcctcaacg acatggtgga tgaaagcgac cgcctgattc ggctcgtcaa
      121 tgatctgctg caacttgccc gcgccgatgc cggtcgtagt ttagtcaaag agagtgtgga
      181 aatttccccc ctgcttcaag aggttgttcg gcaggcacag ttgctcgacc cacagcgtac
      241 gattcaactc aatgcggcag atccacttca agtcagaggg gaccgcgatg cgattaagca
      301 ggtgcttatc atcggattgg ataatgccct taaacattca tcgagccagg tcgaagtcat
      361 ctgcaaaaaa ttggatggac gggtggagat tcagatccaa gatcacggcg atggaattcc
      421 acccgaaaaa ttggaacatg tcttcgaccg tttctatcgc ggcgaagatt ctgcatccgt
      481 ccccggcttt gggctggggc tgtccattgc caaatcccta atcgagagac aggatggcac
      541 tattcaaatg aatagccagg taggtaaagg tagtgtgctg aaattaagat taatgatctc
      601 aagcaattga acttattgtt catcattagg gcatattgcc acgttgcact gacctttatc
      661 tatctgcaaa tactggggta tttgccagat aataactttc gcccgcacat gatttacaca
      721 gagtagattc attaagcaat atctcgcgcc cgtttattac ctcttctcca cagatgccac
      781 agagaatctt tttccctggc ttgcttataa cttcagagag cggcgtaatt aaacagacct
      841 tctgcacatg aaataattta tcaaatggca tgatctgata tgcctccagc atggcctccc
      901 agtggttttt tgcgtgagat gcatattcat ccgcctgagt cctggcttca cgctgtgggg
      961 caatacggag cgaagtttca gtgtgggtat cggtaaaggt ggcagcaact tttccaaagt
     1021 ccaggatgcg aagcgtgcgg ctgccaaccc ggcaatgact ggcggcgatg accccatcca
     1081 ctgcgcagcc gtcggtctca gaagtaacaa gcagccgctt gtcttctctt ggcaattcaa
     1141 gccccagtaa ttccccggca tacaatgcca tacgcgcgcc cagcacttgg cgcggacaaa
     1201 ggtgtttatg gcggcttgag ctttccagca atatttccgg caggttcatt tttcttccaa
     1261 tggggcaaag ccaccgccta ggatctcctg tgcaggcgaa acgatcacta ctgccttagg
     1321 atcttcctta gcgaccgcgg acttgagtgc gtgtgcttct gtgatcgtta acgcacacat
     1381 caaaattgac cggtctttcc cggtgtacat ccccttgcct tccatggatg tgactccacg
     1441 tttcaggtca ttcatgatcc gactagagac ttcttttgct ttatccgtaa taatgagtgc
     1501 cagcagctgt ctgcgtcttt tcctgggaac aagcagcgaa cgcaatcccg tatgtttgcc
     1561 ttccatggat ggtgcggcgg gtgcggtctc atcaccgaag cgcggcagga cttcacctgt
     1621 agccttggtc atgccgactg tgccaatggc aattatcaaa aggaaaagat gatatgccag
     1681 cccaagcgaa atcatgacaa atcctggtat ggatcccact gccccgcgcc caacaaattc
     1741 tgcaatagtg gtggcgggct cgggaaaaag aaaacttttc cccatccacg aaatataaag
     1801 gatcaaatag atccaaaggt agttacgccg caagcggcga ccgaacgcct cccacatgga
     1861 aattggaaaa cttggcgtga gcaaattctc tgccagattt tccgcccact ctggtgaggg
     1921 atgaaacggc ggcacaagca tgggagcata aaaatccgtt tccatcaagc ggacgcgata
     1981 actccatagt tcataataac gataacggcg cgcttcaata aataaaaacc atgtaaccaa
     2041 taatgtattt aaaataataa cgccgtgatg tacattgggc tgactgaacg aaatggaaag
     2101 cgttgcacct gtagcaacga ccgcccagtt ggtggttgta tccaagcgct gacgccagac
     2161 attggcacgc tgaacttctg cacggaagag atgtaccatg gcagtgacaa attcactgct
     2221 cttgagtttg taaccgcgat acgtccaaac agattcttct tcctgtgctg gtggcggcgg
     2281 gggagtttct ttttttctgg gcatgagatt tatccgatcg gcataagaat ggggagaatg
     2341 atcaaaagaa taatgacaag cagaatcgtg agcgggatgc ccacctttgc ataatctttg
     2401 aaggtataac cgcccgcgcc catcaccatc acattgacgg cgtgcccaag cggtgacatg
     2461 aacgccatag acgacgccaa cgcgaccgcc atggcaacgc tgcgcggctc catcccgacc
     2521 tgctgactgg ttgcgattgc gatgggtgcg atcacagtga cagccgccgc gccattgatg
     2581 atctgagtca aagcaacggt cagcaagaca aaactcgcca gcaacaccag atgacttgca
     2641 ttcccaaacg cagacaagat ggcatctgca aacagcgtgg acgcacccgt tttatttaag
     2701 gcgacgccaa gcggcaacat gcctgcaacc aaaaatatgc tgcgccagtc aatggagcga
     2761 tacgcttgat ccatgctcat ggtgccgata agcaccatca tcacagaccc tgtcagcatg
     2821 atctccgcga tgttgtcgga gaataggact gccagcgcca atgttgcaaa catgatcaga
     2881 accgtcagcc aattgcgcca tgtcatgcgc gtggacggag ccgactcagc aagcagaacc
     2941 agacctggct ccgttcgcaa caggcttaaa cttttttcag tgccttgcat caaaaaaccg
     3001 tcaccaaatt cgagcggcag atctgccaag cgtgtgcgga tggaacgtcc gcgccgccaa
     3061 atggctaaga cctgcgagtt gaacttctgc tcaaagcgaa tttcagacaa ggtctgcgaa
     3121 gcgagatgag agcgcggcgc gatggcggct tcgatcaatt tcatattggg tgtggaaaga
     3181 taatcctgct cccattcgcc cgaaggcagc acttccagca tctcccccag cgattcgggc
     3241 acgctatctt cagggcgagc catgatcagc aaaatgtcat cgcctttcaa aatcatcttc
     3301 gcttcaatag gcaaagtgtt tcgtccgcgt tgaaccgcga tcacattgag atgatgtttt
     3361 tcgcgcaagc cgcttttttc gagagtcaca ccgtccaaac tgccgccctt gcaaacctcc
     3421 gcgcgcacca gccgttcagc aagttgatag gtcttgatca attcatccga agcattttct
     3481 tcttcatgcc gttgcgcagg cgattgactt ggtaataatt ttcgtcccca taagaccatg
     3541 aacatgattc ccgcaaaagc cagcggcaac ccaactggta aaaaatcggt caatgaaaag
     3601 gcagataatt tcgcgtcgcg caacagacca ctcaccacaa ggttgaccgt tgtcagcagc
     3661 gttgccatgc cgcccaaaat ggttccgaac gccaacggca tcagcaattt cgacggcgat
     3721 attttcatct tccgcgccac gccagaaagc gatggaaaca gcaacgaagc cgccgcgatg
     3781 ttattcataa aaagcgacaa caccgcagaa gcggtcatca cgccaaagat caatcgtcgc
     3841 tcgccagcgc caaacaattt gataatgaac aaaccgatcc gctcggtcat gccagagcgg
     3901 cgcaatcctt ccgcaaggac gaacgcagac agcatgatga tgaccgcctg tctgctgaat
     3961 ccagaaaagg cttcctgcgg agtcaaaatt ccgctcaagc ccagggcgag catcaacatc
     4021 aacgcgacaa tgtcggctcg taggatgttt ctcagcaaca ggatggctgc aatgccgagg
     4081 aatattaaag ttaaagtgat ctgtgacata ttggataagc gcttcgacaa tcgctcagcg
     4141 ccgcttcggg actcagccga agttgattct gtttcttgaa ttttttctta cgctgcaagt
     4201 gagtctacac ccgcagcgta gattgcttcg taaggctttc gttacacttc aacgtaccaa
     4261 acctgacagg tttcaaatgc tacacttcaa tcatgcgcct ttgtccgcgc aaaagttatg
     4321 aaaggaaaac ttctgcggaa acctgtcagg tttttctttt acttcggcaa tttatatgct
     4381 tc
//
LOCUS       DAOREF010000130         4366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11768_length_4366_cov_2.623057, whole genome shotgun sequence.
ACCESSION   DAOREF010000130 DAOREF010000000
VERSION     DAOREF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11768_length_4366_cov_2.623057"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKI33_03845"
     CDS             <1..907
                     /locus_tag="PKI33_03845"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNM36166.1"
                     /translation="VAAGADVVCLVRDWVPQSELARTGLIEKVKVVRGDIRDREILER
                     TLGEYEIDTVIHLAAQTIVTIANRNPVSTFETNIAGTWNLLEAARRSPKVKQLVMASS
                     DKAYGDQEILPYDEHTPLQGQHPYDVSKSAADLIASTYAKSYDLPVAITRCGNFYGGG
                     DLNWNRIIPGTIRSILRGQRPIIRSDGQYIRDYFYVEDGAAAYMLLAEQLAANPNLKG
                     ESFNFSNEIQVAVLEIVSKILRLMNSDLKPQVLNEASNEIRHQYLSAEKARRLLNWHP
                     LFNLDQALSSTIDWYKEFFTHERTI"
     gene            891..2207
                     /gene="rfbH"
                     /locus_tag="PKI33_03850"
     CDS             891..2207
                     /gene="rfbH"
                     /locus_tag="PKI33_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNM36167.1"
                     /translation="MSERSDRLRAKILELTAEYVAEEFPEKEFIPGESSVAVAGRVFD
                     AADVQSLVDSSLDFWLTAGRFSRQFERDFAKFFNLRHSILVNSGSSANLLALTCLTSP
                     TLKERQLKPGDEVITVATGFPTTVNPIIQNNLVPVFLDVDIPTYNIDVSQLDAALSSK
                     TRAVMVAHTLGNPYKLEAVTAFCKKHNLWLVEDCCDAVGATYNGKLVGTFGDVATVSF
                     YPAHHITMGEGGAVLTDQPPIKKLLESFRDWGRDCWCEPGKDNTCGKRFDWQLGDLPH
                     GYDHKYTYSHIGYNLKVSDMQAAIGVTQLKKVNSFIEKRRANFAYLKNKLSTLQEFLL
                     LPEATPNSEPSWFGFPIFVRESAPFTRDELIHHLNDQKIGTRLLFSGNLLRQPAYRNI
                     THRVVGDLKRSDDVMHRVFWIGVYPGLTEVMLDYVAETIQKFCETR"
     gene            2207..3241
                     /locus_tag="PKI33_03855"
     CDS             2207..3241
                     /locus_tag="PKI33_03855"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM36168.1"
                     /translation="MRLPEADLTHILDHTRSLWDDLRGARLFITGGTGFFGIWLLESF
                     LHANKQLDLKASASVLTRRPDSFQQAFPYLGLDSAIQLVRGDVRNFDFPAGGYSHIIH
                     AATDASEKLNNEEPLLMFDTIVDGTRHALDFAVHCKADRVLFTSSGAVYGKQPPEISH
                     TPEDYIGTPDPLDPRAAYANGKRAAEHLCALYAKQHNVKPVIARCFAFVGPHLPLDIH
                     FAIGNFMRDAMNGGPIIIKGDGTPRRSYLYAADLAIWLWTLLLRGEVCRAYNVGSEES
                     VSIAETAHAVARSVNPEIQIEIRGVPNPELPVEQYVPSTQRVELELGLRDSVRIDEKI
                     SRTVEWLRIR"
     gene            3270..3995
                     /locus_tag="PKI33_03860"
     CDS             3270..3995
                     /locus_tag="PKI33_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM36169.1"
                     /translation="MDTNEKKMLTIMMPAYGEAENLRMLLPEIKTIILELNVPTEVLV
                     LDADIPKDDTKEVCIEHGVKWMPRQGGNRYGDAVRTGIRACKGDYVITMDSDGSHNPQ
                     FIRELWKHRDDADVIIASRYVPGGVTENPWLLVVMSRFLNVIFKAFVGIPVFDVSNSF
                     RLYRGNLLRMISPEYANFDVLEEILARIIWGESGRSARILELPFRFEQRKFGKPKRQL
                     IVFGLQFLTALFKLRALRSRYKF"
     gene            4039..>4366
                     /locus_tag="PKI33_03865"
     CDS             4039..>4366
                     /locus_tag="PKI33_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36170.1"
                     /translation="MTFLSALLLIVTMWIFGHEQLGGFDMSALVDTGWRMANSQTPYV
                     DFPLTTPVAFYVGAGWALKFFGVKWSSFVIAAIIITIATFLAQNYLLNQIMDWRPALA
                     ISLVSQM"
BASE COUNT         1065 a         1073 c         1046 g         1182 t
ORIGIN      
        1 cgtcgcagca ggtgcggatg tagtttgtct cgtgcgcgat tgggttccgc aaagcgaact
       61 ggcgcggaca ggtttgatcg aaaaagtaaa agttgtgcgc ggcgacattc gtgaccgtga
      121 aattttggaa cgtacattgg gtgaatatga gatcgatacg gtcattcatc ttgccgccca
      181 gaccattgtg actattgcaa accgcaaccc tgtttctact tttgaaacca atattgccgg
      241 aacatggaat ttacttgaag ctgcacgtcg cagccccaaa gtaaaacaat tggtgatggc
      301 atcttcagat aaagcctatg gcgaccagga aattttgccg tatgacgaac atacaccgct
      361 tcaaggacag catccatatg atgtcagcaa atctgcggcg gatctgatcg cctcgaccta
      421 tgccaagagc tacgatctgc cggtagcgat tacacgctgc ggaaatttct atggcggcgg
      481 cgacttgaat tggaaccgca ttatccctgg taccatccgc tctatcctgc gcggacaacg
      541 ccctattatc cgctcagatg gacaatacat ccgtgattat ttttatgtgg aagatggcgc
      601 agctgcttat atgcttttgg ctgaacagct tgctgcaaac ccaaacctca aaggcgagtc
      661 gtttaacttt tctaatgaaa ttcaggttgc ggtattggag atcgtctcaa aaattttacg
      721 tctaatgaac tcagacctca aaccgcaggt cttgaatgag gcctcgaatg agatccgcca
      781 tcaatacctg agcgcagaga aagcccgccg cctcttaaac tggcatccac ttttcaattt
      841 ggatcaagcc ttatcatcta ccattgattg gtacaaggaa ttttttacac atgagcgaac
      901 gatctgatcg actgcgggcg aagatcctcg aattgaccgc tgaatatgtc gccgaggaat
      961 ttcctgaaaa ggagttcatc cctggcgaat catccgtggc ggtggcgggg cgcgttttcg
     1021 atgcggcaga cgtgcaatca ctggtcgact ccagcctcga tttttggctg actgccgggc
     1081 gtttctcacg ccaatttgag cgcgactttg cgaaattctt taatctgcgc cactccattt
     1141 tggtgaactc gggttcttct gccaatttac ttgcgttgac ctgtctcaca tcgccgactc
     1201 ttaaagaacg tcaactcaaa cctggcgatg aagtcattac cgttgccacg gggtttccca
     1261 ccacggtcaa ccccatcatt cagaacaatc ttgtcccagt tttcttggat gtggatattc
     1321 ccacatacaa cattgacgtt tctcaattgg atgcagcttt gtccagcaag actcgtgccg
     1381 tcatggttgc acatactctt ggtaacccat ataaacttga agcagtcact gcgttttgta
     1441 aaaaacataa tctctggctt gttgaagatt gctgtgacgc tgttggtgcc acgtataacg
     1501 gaaagctggt tggcaccttt ggcgatgttg ccacggtcag tttttatccg gcacatcaca
     1561 tcacaatggg ggaagggggg gctgtcctca ctgatcagcc gccaatcaaa aaattactcg
     1621 aatctttccg tgactggggg cgtgattgct ggtgcgaacc cggcaaagac aacacctgcg
     1681 gcaaacgctt cgactggcaa ctcggcgatc tgcctcacgg ctatgaccac aaatacacct
     1741 actcacacat cggctacaac ctcaaagtat ctgacatgca agccgcaatt ggcgtcacac
     1801 agttgaagaa ggtcaactcg ttcattgaaa agcgccgcgc gaattttgca tacttgaaaa
     1861 ataaactctc taccttgcaa gagtttctgc ttttgccaga agccacacca aactccgaac
     1921 cgagttggtt tggcttccct attttcgttc gtgagagcgc tccattcaca cgtgatgagc
     1981 ttatacatca tcttaacgat cagaagatcg gcacacgcct gctttttagc ggcaaccttc
     2041 tgcgtcagcc tgcctatcgc aacattactc atcgcgtcgt cggtgatctc aaacgttctg
     2101 acgatgtcat gcatcgcgtc ttttggatcg gcgtttaccc cggtttgact gaagtaatgc
     2161 ttgattatgt tgccgagacc attcaaaaat tctgtgagac tcgttaatgc gcctgcccga
     2221 agccgatctc acacatatcc tcgatcatac ccgcagtttg tgggatgacc ttcgcggtgc
     2281 gcgccttttc attacaggag gcacaggttt tttcgggatc tggcttctag aaagttttct
     2341 gcatgcgaat aaacaactgg acttaaaagc ttccgcctct gttctgacgc gcagacctga
     2401 ctcattccaa caagcctttc cttacctcgg gctggactct gccattcagc tcgtccgtgg
     2461 agatgtacgc aattttgatt ttccggctgg cggctactca cacatcatcc acgccgcgac
     2521 ggatgccagc gagaaactta acaacgaaga accgcttctg atgttcgata ccattgtgga
     2581 tggcacgcgc catgcactgg attttgctgt ccattgcaaa gcggatagag ttctcttcac
     2641 tagttctggt gcggtttatg gcaaacagcc cccagagatc tcacataccc ccgaagatta
     2701 catcggcaca cctgacccgc tcgacccacg tgctgcttat gccaacggaa aacgtgctgc
     2761 tgagcacctt tgcgctttgt atgccaaaca acacaacgtt aagccagtca tcgcacggtg
     2821 ctttgcattt gttggtccac accttccttt ggatattcac tttgccatcg gtaatttcat
     2881 gcgcgatgcc atgaacggcg gaccgatcat tatcaaaggt gatggcactc cgcgccgctc
     2941 ctatctctac gccgccgacc tcgccatttg gttgtggacc ttgcttctgc gcggagaagt
     3001 atgccgggca tacaacgttg gcagcgaaga atcagtgtcc attgcagaaa cagcccacgc
     3061 cgtagcgcga agcgtgaatc cggaaatcca aattgagatt cgcggagttc caaatccaga
     3121 attgcctgtt gagcagtatg ttccatccac gcagcgggtg gagctggaat tagggttgcg
     3181 ggattctgtt cgcattgatg aaaaaatatc tcgaacagta gagtggcttc gcattcgata
     3241 aattatggaa aggagatggg agatatagca tggataccaa tgaaaagaaa atgctgacga
     3301 tcatgatgcc tgcttatggc gaggctgaaa atctgaggat gttattgcca gaaatcaaga
     3361 caattatcct tgaattgaat gttccaactg aagtgctggt attggatgcc gatattccaa
     3421 aagatgacac taaagaggtt tgcattgaac acggggtgaa atggatgccg cgtcagggtg
     3481 gcaaccggta tggggatgct gttcggacgg ggatccgagc ctgtaagggc gattatgtga
     3541 tcacaatgga ctctgacggt tctcataacc ctcaatttat tcgcgagctt tggaaacatc
     3601 gcgatgatgc cgatgtgatc attgcatccc gatatgtgcc gggcggcgtg acggaaaacc
     3661 cctggttgct tgttgtcatg agccgttttc ttaatgtgat atttaaggcg ttcgttggca
     3721 ttcccgtgtt tgatgtgtct aatagttttc gcttgtaccg tggcaacctt ctgcggatga
     3781 tctctccgga gtatgcaaac tttgatgtgc tcgaagagat cttggcaaga attatttggg
     3841 gagaatccgg gcgctctgca aggatactgg aattgccttt tcgatttgaa cagagaaaat
     3901 ttggcaaacc aaaacggcag ctgatagtct ttggtctgca gtttttgact gcgcttttca
     3961 agctgcgggc tcttcgatca agatacaaat tttagtgacg taatgaaacc aatgtctata
     4021 ttcgctcgca aacctgtttt gacttttcta tcagcattat tgcttatagt aactatgtgg
     4081 atatttgggc atgaacagct tggcggtttt gatatgagcg cactggttga tactggctgg
     4141 cgaatggcga actctcaaac cccatatgtg gatttcccac ttacgacccc ggtcgctttt
     4201 tatgttggtg caggctgggc tctcaagttt ttcggcgtta agtggtcatc atttgttatt
     4261 gctgcaatta tcattacgat agcaaccttc cttgcacaaa attatcttct taatcagatc
     4321 atggattggc ggccagcgct cgctatttct ctagtatctc aaatgc
//
LOCUS       DAOREF010000131         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11791_length_4361_cov_3.990014, whole genome shotgun sequence.
ACCESSION   DAOREF010000131 DAOREF010000000
VERSION     DAOREF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11791_length_4361_cov_3.990014"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKI33_03870"
     CDS             <1..429
                     /locus_tag="PKI33_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HNM36171.1"
                     /translation="KMTQQAIQKVFETYQAAAYAKDVEAFLAIYHQDVHIFDMWEKWS
                     YDGIAAWRGMVTGWFGSLGTELVKIEFNEMQITETKEMAIAHMIVTFRGLSAEGTELR
                     SMQNRMTCVLKNINGAWQIIHEHSSSPLEPSSLKAILHKA"
     gene            complement(464..829)
                     /gene="acpS"
                     /locus_tag="PKI33_03875"
     CDS             complement(464..829)
                     /gene="acpS"
                     /locus_tag="PKI33_03875"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNM36172.1"
                     /translation="MILRTGVDLIETNRIADAIERHGERFLNRVFTAHELSECKGRVP
                     SLAARFAAKEAVAKALGTGIGDVSWLEIEVRSDEKKAPYVVLSGEGEKLAKKLGLGTW
                     SLSLSHTAAQAMAFVVATE"
     gene            complement(834..2108)
                     /locus_tag="PKI33_03880"
     CDS             complement(834..2108)
                     /locus_tag="PKI33_03880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNM36173.1"
                     /translation="MATKTKSSMPSPEDVYQTTLPNGITVLARSNYNSPSINVGGYLP
                     AGAIFETDEKLGLADFVTAMLMRGTQTRTFDSIYNELETVGASLGFDSGVHNASFGGR
                     ALVEDLPLLMKLLSESLRTPTFPKDEMERLRTQILTGLALRAQDTSDMADLLYEQFLY
                     ADHPYGKPSDGYVETIQAISRKDLETFHRLHFGPRGMVITIVGAIQPKKAVEAVAKFL
                     GDWTVPSQKEPPVLPPLKPLKKTVRKHHQILGKSQSDLVVGTIGPTRKSPDFMAASLG
                     NSVLGQFGMMGRIGHVVREKSGLAYYAYSSLHSGVGPGSWEVAAGVNPKNLKKALDLI
                     DVELRRFVKNGVTKEELADSQANYVGRLPLSLESNGGMVSAILNIHRYELGMDYYQRY
                     ENMVRSVTRDDVVETARKYIDPDRLVIATAGP"
     gene            complement(2108..3370)
                     /locus_tag="PKI33_03885"
     CDS             complement(2108..3370)
                     /locus_tag="PKI33_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNM36174.1"
                     /translation="MHKNITQVQLKNGMKIMLKEIHTAPIISSWIWYKVGSRDESTGK
                     TGISHWTEHMMFKGTKKFPGGILDKAISREGGRWNASTSRDSTRYYETMPAAKIDLAL
                     RLESDRMTKSIFDAKEVASERTVIISEREGSENEPMFSLGEAVQHASFRVHPYHHEII
                     GDMADLRTITRDDLYNHYRSYYVPNNAVLSLAGDFDTKSMLKRIKELFEPIPKGNVPP
                     RLARPEPERKGELRLTVEGPGETAFTQVAYPFPNATHPDYFALQVLESLLNGASGLSY
                     KTCRLYRALVDKGHVVDISGWSESTIDPFLYRITLVHRPDKKPEEALAVLDDEIKKVQ
                     DKPVSAAEIQRAVKQARAVFAYGSENITHQAFWMGYTSMFSDYSWYTTYLDKLAKVTP
                     KDVQRVAQIYFQTSKRVVGNYIPKGKAA"
     gene            complement(3430..>4361)
                     /locus_tag="PKI33_03890"
     CDS             complement(3430..>4361)
                     /locus_tag="PKI33_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36175.1"
                     /translation="TDEPIKEDPVQKFRKLIASFIPLSRLPKKSTQAEPKEELPVEEI
                     KEEVKVEENKKDALSALPKKSTPQNDMLGSLPKKGALPPKKAEPKPVETPAPAPSPAS
                     TPTSKLAPASSSGYSVWGPRLWTTASVISLTINIILMIVLIILVISVYRLKLDMAKIM
                     DASTSVMGLPAGLYSNFEKMERASIQTNVEVNTSIPVKFDLQLNQQTNVVLSQDVTIQ
                     NARVTVNTGGLNISQANTTIVLPAGTTLPVNLALTVPVDQQVPVTLNVPVDIKLKDTQ
                     LNDPFVGLQEVIKPLYCLLEPTAVNMDGNPICQ"
BASE COUNT         1069 a         1046 c         1104 g         1142 t
ORIGIN      
        1 aaaatgactc aacaggctat tcaaaaagta tttgaaacct atcaagctgc tgcatatgca
       61 aaagatgtgg aggctttttt agccatctat caccaagacg tccatatttt tgatatgtgg
      121 gaaaagtggt cgtatgacgg aattgctgcc tggcgtggaa tggtgaccgg ttggtttggc
      181 tcattaggca cggagttggt aaagattgaa ttcaacgaga tgcaaatcac cgagacgaaa
      241 gagatggcaa ttgcccatat gattgtcacc tttagagggc tatctgctga gggcacagaa
      301 ttgcgctcca tgcaaaaccg aatgacttgt gtgctcaaaa atataaatgg cgcatggcag
      361 atcatccatg aacatagttc ttccccgctg gaaccaagtt ctctaaaagc tattctccat
      421 aaagcctaaa aagagagcct tccaaatatt ggaaggctct cttttattca gtagcaacaa
      481 caaatgccat tgcctgtgct gcggtatggc tcaaactgag agaccaagta cccaacccaa
      541 gtttttttgc caacttctcc ccttcgccag ataaaaccac atagggcgct ttcttctcat
      601 ccgaacggac ttcgatttcc agccagctta catcgccgat tcctgtgccc aatgcttttg
      661 ccacagcttc ttttgccgca aaccgcgccg ccaaagatgg gacacgccct ttacattcac
      721 tcagttcatg cgcggtaaaa acacggttca ggaatcgttc gccgtgccgt tctatggcat
      781 cagcgatacg gttcgtttct attaagtcca caccggttcg caagatcatt acgctacggt
      841 cctgctgtgg caatcaccaa acgatcgggg tcaatatact tgcgagcggt ttccacaaca
      901 tcatctcggg tgacactgcg caccatgttt tcgtaacgct ggtaataatc catgcccaac
      961 tcgtagcgat gaatattcaa aattgcgctc accataccgc cattcgactc aagtgaaagc
     1021 ggcaaacgtc ccacataatt tgcctgacta tcagcaagct cctctttagt cacgccattc
     1081 tttacgaagc gccgcagttc aacatcaatc aggtcaagcg cctttttcag gttcttcggg
     1141 ttgactccag ccgccacttc ccaactgcct ggtcctaccc cagagtgcag acttgaataa
     1201 gcataatatg ccaagcccga tttttcacgc actacatgcc caatgcgccc catcataccg
     1261 aactgaccca agaccgaatt cccaagagac gccgccatga aatcagggct tttgcgtgtg
     1321 ggtccaatcg tgccaacgac caaatctgat tgggatttgc cgaggatttg atgatgctta
     1381 cggacagttt tcttcaacgg cttaagcgga ggaagaacag gcggctcttt ctgacttgga
     1441 actgtccagt cgcccaagaa ttttgccacc gcctcaaccg cttttttcgg ctggatagcg
     1501 cccacgattg taatgaccat gccgcgaggt ccgaagtgga ggcgatggaa ggtttccaaa
     1561 tctttgcgtg agatcgcctg aatggtctcc acatatccat cagaaggttt gccataggga
     1621 tgatcggcat acaggaattg ctcatagagc aagtctgcca tatcagaggt atcttgagca
     1681 cgcagtgcca gcccggtgag aatttgagtg cgcagacgtt ccatttcatc tttcgggaac
     1741 gtgggagtgc gcagcgattc agagagaagc ttcataagaa gcggaaggtc ttcaaccaag
     1801 gcgcgaccgc caaaactggc attgtgaaca cccgagtcaa agccgaggct cgcgccgaca
     1861 gtttccaact cgttgtaaat ggagtcaaac gtgcgggttt gggttccgcg catgagcatg
     1921 gcagtgacaa aatctgccag accgagtttt tcatcggttt caaaaatagc gccagcagga
     1981 agatacccac ccacattgat agatgggctg ttgtaattgg agcgcgccaa aacagtaatg
     2041 ccattgggaa gtgtggtctg gtacacatcc tcagggctgg gcatggaaga cttggtttta
     2101 gttgccatta cgccgccttc cctttgggaa tgtagttccc cactacacgt ttactcgtct
     2161 ggaaataaat ctgcgccaca cgctgcacat ctttgggcgt gaccttggct agcttatcga
     2221 gatacgtggt gtaccaactg taatctgaga acatggaggt gtagcccatc caaaaggctt
     2281 gatgagtaat gttctcactg ccataagcaa aaacagcgcg cgcctgcttc acagcacgtt
     2341 gaatttctgc cgcagagaca ggtttatctt gcaccttctt gatctcatca tctaacactg
     2401 caagcgcttc ttcgggcttt ttatcagggc gatgcaccaa agtaatacga tataagaacg
     2461 ggtcaatggt ggattcggac caaccagaaa tatctacaac gtgtccctta tctacgaggg
     2521 cgcggtacaa acggcaggtt ttataggaaa gtccgctggc gccattgagc aagctttcaa
     2581 gaacttgcaa agcgaagtaa tcaggatgtg tggcatttgg gaagggatac gccacctgcg
     2641 tgaacgccgt ctcgccagga ccttccaccg ttaagcgaag ttcgcctttg cgctctggtt
     2701 cagggcgggc aaggcgcggc gggacatttc ccttcgggat cggctcgaac agttccttga
     2761 tgcgtttgag catggatttg gtatcaaaat ctccagccag cgaaaggaca gcattgttcg
     2821 ggacatagta tgaacgatag tggttgtaaa ggtcatcgcg ggtgatggtg cgcaaatctg
     2881 ccatgtcacc gatgatttca tgatgatacg ggtggacacg gaatgaagca tgttgcaccg
     2941 cctcccctag tgagaacata ggctcattct ctgacccttc gcgctctgaa atgatgaccg
     3001 ttctttcaga tgccacttct ttggcatcga aaatgctctt ggtcatgcgg tctgattcga
     3061 ggcgcagggc aaggtcaatt ttggcggcgg gcatggtttc gtaatagcgg gtggaatcgc
     3121 gagaagtgga tgcgttccaa cgtccgcctt cacgggagat ggctttatcg agaataccgc
     3181 ctgggaattt cttcgtccct ttgaacatca tgtgttctgt ccaatgagag atgccggttt
     3241 tcccggtgga ctcatcgcgc gagccgactt tgtaccagat ccacgacgag atgatgggtg
     3301 ccgtatggat ctctttgagc atgatcttca tgccattctt aagctggacc tgcgtgatgt
     3361 ttttatgcat ttaactccaa agtattttct gttattttgc atcctcagac ggtgcacaac
     3421 aagcaagtat tactggcaga tcgggttgcc gtccatattg acggcagtgg gttcgagcaa
     3481 acagtaaagc ggtttgatga cttcttgtaa gccaacaaag gggtcgttca attgtgtgtc
     3541 tttgagcttg atatcaacag ggacgttcaa ggtcactggc acttgctgat ctacggggac
     3601 ggtaagggct aagttgacag gtaatgtagt gcccgccggg aggacaatgg tggtgttggc
     3661 ttgcgaaata ttcaacccgc cggtgttgac ggtgacacgc gcgttttgaa tggtcacatc
     3721 ttgactcaag acgacattgg tttgttggtt gagttgcaaa tcaaatttga ccggaatcga
     3781 ggtgttgacc tctacattcg tctgaatgct ggcgcgttcc atcttttcga agttggaata
     3841 taagccagcg ggaagtccca tcacggaggt ggaagcgtcc atgatcttcg ccatatctag
     3901 tttgagacga tagacgctga tcaccagaat gataagaacg atcatcagaa tgatgttgat
     3961 cgtaagagag ataacgcttg ccgttgtcca taaacgagga ccccaaacgg agtatccgct
     4021 actcgaagca ggtgccagtt tcgaggtcgg ggttgaggcg gggctggggg caggagccgg
     4081 agtctctacc ggtttcggtt cagctttttt aggcggtaac gctccctttt tgggtaacga
     4141 ccccagcata tcattttgtg gcgttgattt tttgggtaaa gctgaaagag cgtctttttt
     4201 attttcttcc actttgacct cttcttttat ttcttccacg ggtagttctt ctttaggctc
     4261 tgcctgtgtt gattttttgg gcaggcgtga gagtggaatg aaactagcaa taagctttcg
     4321 gaatttttgt acaggatctt ctttgatggg ttcgtctgtc a
//
LOCUS       DAOREF010000132         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11846_length_4350_cov_5.308964, whole genome shotgun sequence.
ACCESSION   DAOREF010000132 DAOREF010000000
VERSION     DAOREF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11846_length_4350_cov_5.308964"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1020
                     /locus_tag="PKI33_03895"
     CDS             133..1020
                     /locus_tag="PKI33_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36176.1"
                     /translation="MTADLSSTPIKSNSPGVRLLRQILRNRSAQFGGILLILLIVASY
                     AAPFITAKDPNKNAPSVALEAPSFEYPMGTDNLGRDNFTRFLYGGQLSLRVGLTGIII
                     GALIGILLGLISGYFGGWLDSFISWFNDVLMAFPDILLALAIIAILGPGINNAMLAIG
                     ISFIPSFMRLTRSSVFALREMTYIEAAKALGSSDTRIMLKHILPNSIRTLLVLITLGI
                     GSSILAGAALNFLGLGAEPPTAEWGAMLNAGMKYVRQAWWLTFFPGLGIFLAVLSINL
                     IGDAVSDAVAGTTIIKESK"
     gene            1022..1708
                     /locus_tag="PKI33_03900"
     CDS             1022..1708
                     /locus_tag="PKI33_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM36177.1"
                     /translation="MASLSGKTAIITGGSTEIASSVALKLAEAGANICLCGRQADLLA
                     MAVDRVKQAGGKGFFVESNLKSLEEAEGVVHQTTAEFGGLDILILISPFWSGGMIHSH
                     SVKTWDLVMDANLREPFLMSRAALPFLREQKHGEVMTIGSDSALGVYPQDGAFAVAMH
                     GLTTLMELIRAENSEHGIRTHILSPGVAVSAPFDVEGKPNLMPDHVADWVLWLLTRPD
                     HLRGNSPILI"
     gene            complement(1754..>4350)
                     /locus_tag="PKI33_03905"
     CDS             complement(1754..>4350)
                     /locus_tag="PKI33_03905"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNM36178.1"
                     /translation="HGANELIERGGRTPWQILWEQITATMVLILIAAAVVAGVLGDTK
                     NTIAILSIVALYALLGFIQEYRAEQAIAALKKMSVPNVRVIRDGKMQELSARELVPGD
                     IIQLETGNVIPADLRLVEAVNLRIQEAALTGESEAIEKHVAALSNEDLPLGDRRNMAY
                     MGTVITQGRGLALVTATGMNTELGKIADMIQAVKQEQTPLQRRLDALGKTLALIGVGI
                     AVLIFILGIIRGDEVRHMLLTAVSVAVAIVPEGLPAVVTITLALGAQRMLQRQALIRK
                     LPAVETLGSVTVICSDKTGTLTENRMTVVVLDVAEHALDLTGDVARDGSLHATRGLGA
                     PAQSSLSLAAIGGALCNDAKLVDEGDDRFHTMGDPTEGALVVAAAKMGYWKSSLDSSF
                     PRAAELPFDSDRKRMTTVHHLGQYDPTVLSGLEIGDTRYIAFTKGGVDGLLDIASHVW
                     ADGKSVPLDDAWRSKIEASNERLARKGMRVLGVGFRLINAIPEIIQTDLEQNITLVGL
                     FGMIDPPRNEVKDAVAIAKTAGIRPIMITGDHPLTAMEIARQLGITDNGRVLTGVELE
                     KITMDELKTMLDDVNVFARVAPEHKLRIVQALQEKGHVAAMTGDGVNDAPALRKADIG
                     VAMGITGTDVSKEASDLVLLDDNFATIVAAVKEGRTIYDNIRKFVRFSVAGNIGKVLV
                     MLIAPFLGKPLPLLPLQLLWLNLLTDGLLGLGMGVENPEKDTMKRKPYSPTEGVFSRG
                     AGIQTIWVGALIGALALGLGSWYYFTDRPEWQTMIFTSLAFMQIFQALASRSDKDSLV
                     KIGVMSNPVLAGMALLVFVLQMAVIYIPALANFFEVIPLRWHDLSIAAGTGLLVFLVM
                     EVSKWFKK"
BASE COUNT         1017 a         1113 c         1162 g         1058 t
ORIGIN      
        1 cgtgcaagcc atctacagcc gtgattaccc catggtgcaa ggcgtggtgc tgattacagc
       61 tacaatctat gtgctcatca acaccattac agacatcacc tatgttttac taaacccgaa
      121 gattcgcctc aaatgaccgc agacttgtcg tccactccta tcaaatccaa ttcgccgggc
      181 gttcgtttgc tcaggcaaat tcttcgtaat cgtagcgccc aatttggcgg catcctattg
      241 attcttttga tcgtcgcctc gtatgccgca ccttttatca cagcgaaaga ccctaataaa
      301 aacgccccat ctgtagcctt agaagcgcct agctttgaat accccatggg cacagataac
      361 cttgggcgtg ataacttcac ccgcttcctt tatggcggtc aactgtcatt gcgagttggg
      421 ctgaccggta tcatcatcgg cgcattgatt gggattctgc tcgggttgat ctctggctac
      481 ttcggcggat ggctggattc gttcatctct tggttcaatg atgtgctgat ggcgttccct
      541 gatattttgc tggcattagc catcatcgcc attctcggac ccggtatcaa caacgctatg
      601 ttggcgattg gtatctcgtt catcccttcg tttatgcgtc ttacgcgttc aagtgtgttt
      661 gccttgcgtg aaatgactta catcgaagcc gcgaaagcac tcggcagttc agacacgcga
      721 atcatgctca agcatatttt gccgaactcg attcggactt tgttggtgct catcacactt
      781 gggataggct catctatcct tgcgggtgcg gcgttgaatt ttcttgggct cggtgcagag
      841 ccgcccacag cagaatgggg cgctatgctc aatgccggca tgaaatacgt ccgccaggcg
      901 tggtggttga ccttcttccc ggggttggga attttcctcg cggtgctttc gatcaacctg
      961 attggtgatg cggtcagtga tgcggtagca gggacgacca tcatcaagga aagcaaatag
     1021 catggcatct ttatcgggca agaccgccat cattacgggt ggctccacag aaatagcaag
     1081 ttctgttgca ttgaagctag cagaagcagg ggcgaatatc tgtttatgtg gcagacaggc
     1141 agatttatta gcgatggcag tagacagagt caagcaagcg ggaggtaaag gcttctttgt
     1201 agaatccaac ctcaagtcgt tggaagaggc ggaaggggtt gtccatcaaa ccacggctga
     1261 atttggcggt ttggatatcc tgatccttat ctcgcccttt tggagcggtg gcatgattca
     1321 cagtcacagc gtcaagacct gggatctggt gatggatgcc aatttacgtg agccgttttt
     1381 gatgtcgcgt gcggcgctcc cttttttgcg agaacaaaaa catggtgagg tgatgacaat
     1441 cggctcagac tctgcgttgg gcgtataccc acaagatggg gcatttgccg ttgccatgca
     1501 cggactgaca actttgatgg agctcattcg cgctgagaat agcgagcatg gcattcgtac
     1561 acatattctt tcgccgggcg tggcggtatc tgcgcctttt gatgtggaag gaaaaccaaa
     1621 cctcatgcca gaccatgttg ctgattgggt cttatggttg cttacccgcc cagaccattt
     1681 acgcggaaac agccctattc ttatctaaca aaaaagacct gcttcaaaag caggtctttt
     1741 attttctaac aagttatttc ttaaaccatt tgctgacttc cataacgagg aataccagca
     1801 agcctgttcc tgcggcgata gatagatcat gccagcgtag aggaatgact tcaaagaagt
     1861 ttgccaatgc agggatgtag atgaccgcca tctgaagaac aaagaccaac agcgccatgc
     1921 ccgccagaac ggggttactc atcacgccga tcttgacgag cgagtctttg tctgagcgag
     1981 acgccagcgc ttggaagatc tgcatgaatg ccagagaggt gaagatcatc gtctgccatt
     2041 cggggcggtc agtaaagtaa taccaggagc caagcccaag agcgagtgcg cctatgaggg
     2101 cgccaaccca aatcgtttgt atgccagccc cgcgcgagaa gacgccctca gtcggggagt
     2161 aaggtttgcg cttcatcgta tccttttcag gattttccac gcccatccct aaaccgagca
     2221 aaccatcagt tagcaggttg agccacagta actgcaaagg caagagcggc aatggcttac
     2281 caaggaaagg cgcaatgagc atgactaata ctttaccgat gttgccagct acactaaagc
     2341 gcacaaattt gcggatgttg tcgtaaatag tacgaccttc tttgaccgcc gccacaatgg
     2401 tcgcgaagtt gtcatcgagc agaacgaggt ctgatgcttc tttggaaaca tcggtgccgg
     2461 tgatgcccat cgcgacacca atatcagctt tgcgcagagc cggggcatcg ttcacgccat
     2521 caccggtcat ggcagcgaca tgaccctttt cctgcaatgc ctgcacgatg cgcagtttat
     2581 gctcgggtgc tacacgtgca aagacgttca catcgtcgag catggtcttt aattcatcca
     2641 tcgtgatttt ttcaagctca acgccggtca gcacacgtcc attatctgtg atgcccaatt
     2701 gacgtgcaat ttccatcgcg gtcagtggat ggtcaccagt aatcataata gggcgaatgc
     2761 cagccgtctt ggcgatggca accgcatctt tgacctcgtt acgtggtggg tcgatcatgc
     2821 cgaacaaacc taccaaggtg atattctgtt caaggtcagt ttgaatgatc tcagggatgg
     2881 cattgataag gcggaagcca acgccgagga cgcgcatacc ttttctggca aggcgttcgt
     2941 tcgacgcttc aatcttggac ctccaggcat catcgagcgg aacagacttg ccgtctgccc
     3001 agacgtggct tgcgatgtcg agcagaccgt ccacaccgcc tttggtgaaa gcgatataac
     3061 gtgtatcgcc gatctctaaa ccagaaagca cggtcgggtc gtattgacca aggtgatgca
     3121 cggtggtcat acgtttgcgg tcagagtcaa agggtaattc ggcggcacgg gggaaagatg
     3181 aatcgaggga agatttccaa tatcccatct ttgcggcggc aactacgagc gcaccttctg
     3241 tggggtcgcc catggtgtgg aagcggtcat cgccttcgtc cactaatttg gcatcgttgc
     3301 acaacgcgcc accaatcgct gccagtgaga gagaggattg ggcaggtgca cccagcccac
     3361 gggtcgcatg aagcgagccg tcgcgggcaa cgtcaccggt caggtcgagg gcatgttccg
     3421 ccacatccaa cacaacgacc gtcatccggt tttcagtgag tgtaccggtc ttatcggagc
     3481 agatgacagt gaccgagccg agggtttcga cagcggggag tttgcgaatg agcgcctgac
     3541 gctgcaacat acgttgggca ccgagtgcga gcgtgatggt gacaacggca ggtaagcctt
     3601 ctggcacaat ggcaactgca acactgaccg ccgtgagtaa catgtgacga acttcgtcgc
     3661 cacgaataat accgagaatg aaaatgagca ccgcaatgcc cacgccaatg agcgcaaggg
     3721 ttttaccaag cgcatcaagg cgacgttgaa gcggagtctg ttcttgtttg accgcttgga
     3781 tcatatctgc gatcttgcca agctcggtgt tcatgccggt ggcagtgacg agagcaagtc
     3841 cgcgcccttg agtgatgacc gtgcccatat atgccatgtt gcgacggtcg ccaagcggaa
     3901 ggtcttcatt tgaaagtgca gccacgtgtt tttcaatggc ttctgattcg ccagtgagcg
     3961 cggcttcttg aattctcaag ttgacggctt caaccaaacg caaatctgca gggatgacgt
     4021 tgccagtttc gagttgaatg atatcgccgg gcacaagctc gcgtgcagag agttcttgca
     4081 ttttgccgtc gcgtatcaca cgcacattgg gcaccgacat tttcttgagg gcggcaatgg
     4141 cttgttcggc acggtattcc tgaatgaaac ccagaagtgc atacaatgcc acaatggaaa
     4201 gaatggcgat cgtgttcttg gtatctccca acaccccggc tacgactgct gctgcgataa
     4261 ggatcaacac catggtggcg gtgatctgtt cccatagaat ttgccatggg gtgcgtccgc
     4321 cgcgttcgat cagttcgttc gcgccgtgtt
//
LOCUS       DAOREF010000133         4324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11969_length_4324_cov_4.335442, whole genome shotgun sequence.
ACCESSION   DAOREF010000133 DAOREF010000000
VERSION     DAOREF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11969_length_4324_cov_4.335442"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2628)
                     /locus_tag="PKI33_03910"
     CDS             complement(<1..2628)
                     /locus_tag="PKI33_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNM36179.1"
                     /translation="MTGIFISYSRKDSQAAKKLMEGFKSVKLDVWVDWEDIPPAVGWL
                     DQILHGIEQAEVFVFLISPDSIKSEVCKVELEHARKNSKRIIPVVARDVVVQDVTPIL
                     RDLNWIFIREQDDFAAGLEKVNLAINLDIEWLQEHRRLQVRALEWDRKKEPSLLLRGT
                     DLRNARKMIVANEKKDPFPSPLQKLYIESSRRGEQLRLTTYVSAAVTLFIMVFLSLVA
                     LNQRQEAISQRQVAQENEKIAQEARKEADRNASIARAQKDKALANEKIARAQRSAARA
                     QIYQSRTGGLFTSTLLGIDSYQRNPSVEAEEILRKNISLLPIPVKQIEQNGAILHIEV
                     SPQGTSFVSTSEDGTACLINFESGESIFCVQSSGAVLDAAFSPDGSKLVISDATGEVQ
                     ILNAETGEILKQIDLGVSVRDVNISPDGTLLAMARDDARISLIKLSNYGFAGEFSVYG
                     SLRVTTFSPDGEWFAAASNLGAITFWNLGTGEIVNGGAHRGEVFEIAFSPNSRELISG
                     AADNCAVLTSPSSGKQILKVLNEDRVTNVAFSPDGSWFVTSSDDFRIRVWDSASGAER
                     FRFLQDSVVKEVKVSPDAMWIASTGSDRTARVWSAANGAEMYQIPLDAEGRVLGFSSD
                     TNYLVVGDAEGNLSIWDISSLAMNDGYMRFDEYVSDIAMSPNGDWLAAADKGQVWLLD
                     PELLPSQIVPTGKLLYTFNADTVIDMAVNPKGDSFAVSTQGGQVVFITSQGVTRVVTS
                     SGAQQALAFSTDGSTLFLAGNNGQLQSRSITSGENASVWQGATSIYSIAVSPSEQISI
                     GMDDKIVLLQVGSTTSEIDLPAPGKNMQVIFNADASLLASSTPDGRAYVWQLKNGEYK
                     RIVELTSAPISSIAFSPNGD"
     gene            complement(2739..>4324)
                     /locus_tag="PKI33_03915"
     CDS             complement(2739..>4324)
                     /locus_tag="PKI33_03915"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNM36180.1"
                     /translation="NSNAPFMWEGISYATLNQWCRSTGLDCNGYEDSPGLSTDMTLLS
                     NSPNIDRGVLIPGINDQFMGSAPDLGAYESVYGTSPATPVATNTPFPLPTDTPTATPV
                     YSSPVVTSITLADPTPNTTELVRFFVNFSEPVNNVDVNDFMMTATGSISNYTILDAYP
                     VGSAYAITVYTGVGDGTLRLDLVDNDSILSATSMHLGGVGIGNGNFSLGNVYVINKNA
                     PFVTSILRADPSPSNAGSVRFSITFSESVSGVDAADFVLSTSGLSEAGVASISGSGTA
                     YMVTVNTGIGSGTLRLDLNDDDSVVDSAGAPVGGIGVGNGNFAAGETYTIDRNIPLIE
                     TVSFSSDGAQDGWVMESKETSNKGGTLNSNAITFRVGDNAQDNQYRSILQFSTGALPD
                     NAIITQMILTMQLETIVGTNPFTTHRNMWVDIRQGAFGSFGPLQIGALQISDFQAPAS
                     LYNAGTIQNNAVGGWYWVNFDSGVFSLVNLKGATQFRLGFQLDDDDDRKDDYLSFFSG
                     NYSVQSARPQLTIKYYIPK"
BASE COUNT         1228 a         1085 c          922 g         1089 t
ORIGIN      
        1 atctccattg ggtgaaaatg ctatcgaaga aataggagca ctcgtaagtt caacgatccg
       61 tttgtattcg ccatttttca attgccagac atatgctcga ccatcagggg tgctggaggc
      121 taacaaggaa gcatcagcgt tgaaaataac ctgcatattt ttacctggag caggcaggtc
      181 aatttcagag gtagttgaac ctacttgtag caagacaatc ttatcgtcca tcccaatgga
      241 aatttgttca cttggagaga cagctattga gtaaattgaa gtagctccct gccagacaga
      301 agcattctcg ccagaggtaa tacttcgtga ttgtaactgt ccattattgc ctgcaaggaa
      361 taatgtgctt ccatctgttg agaaagcaag agcttgttgg gcgccagaag atgtgactac
      421 gcgtgttact ccttgggaag taatgaacac gacttgcccg ccttgagtgg acacggcaaa
      481 cgagtcgcct tttgggttta ctgccatatc tatgacagta tctgcattaa aggtgtacag
      541 taatttgcca gtaggaacga tctgagatgg gagcaattcc gggtccagca accagacctg
      601 ccctttgtct gcggcggcaa gccaatcgcc attggggctc atagcaatat cactgacata
      661 ctcatcaaag cgcatatacc catcgttcat ggcaagcgaa gaaatatccc aaatagatag
      721 gttgccttca gcatcaccaa cgacaagata atttgtatca gagctaaaac ctagaaccct
      781 gccttcagca tctaatggaa tctggtacat ctctgcccca tttgcagcac tccacacacg
      841 tgccgttcta tctgagccag tagaagcaat ccacatggca tcggggctga ctttgacttc
      901 tttcacaaca ctatcttgca agaagcggaa acgctcagca ccagaggcag agtcccaaac
      961 gcggatgcgg aaatcatctg atgaggtgac aaaccaagag ccgtcagggc taaatgccac
     1021 attggtaaca cgatcttcat tcaatacttt taatatctgt ttccctgaag aagggctggt
     1081 aagaacagca caattatcgg ctgctccaga gatcaactca cgactatttg ggctaaaagc
     1141 gatttcaaaa acttcgccac gatgcgcacc gccgttgaca atttctccag tgcccaaatt
     1201 ccaaaatgtg atcgcaccca ggttcgaagc agctgcaaac cattcgccat ctgggctaaa
     1261 tgtcgtaact ctcaaactgc catataccga gaattctccc gcgaacccat aattggaaag
     1321 ttttatcaaa gaaatgcggg catcatcgcg tgccatggca agcaaagtcc catctgggct
     1381 gatgtttaca tcacgcaccg aaacgccaag atcaatctgt ttaagaatct cacccgtttc
     1441 agcatttagg atctgcactt cgcccgttgc atcactaata accaatttgc taccatctgg
     1501 actaaacgcc gcatctaaca cagccccaga gctctgcaca cagaatatgc tttctccact
     1561 ctcaaagttg atcaaacaag ctgtaccatc ttcgctggta gaaacaaacg atgtaccttg
     1621 cgggctgacc tcaatgtgca agatggctcc gttctgctct atctgtttga cagggatcgg
     1681 cagcaagcta atattctttc gaagaatttc ctctgcctca acagatggat ttctttgata
     1741 tgaatctata ccgagcaaag tgctagtaaa cagtccacca gttcgagact gataaatctg
     1801 cgctcgtgca gcactacgct gtgctctggc aatcttctca ttggcaagcg ccttgtcttt
     1861 ctgagcacgc gcaatggacg catttcgatc cgcttcttta cgcgcctctt gcgcaatctt
     1921 ttcattttct tgcgccactt ggcgttgact gatcgcttct tgcctttgat tcaatgccac
     1981 aagggaaaga aacaccataa taaacaaagt cactgcggca gaaacgtatg ttgtaagacg
     2041 gagttgttcc ccacgccggc tggattcaat atatagcttt tgcaaaggcg aagggaaagg
     2101 gtctttcttc tcattcgcca cgatcatttt tctcgcatta cgcaggtcag taccgcgtag
     2161 taacaagctg ggttcttttt tacgatccca ttccaaggcg cggacttgca aacgtctatg
     2221 ttcctgcaac cattcaatat ctaggttgat cgccagattt actttttcaa gcccagcagc
     2281 aaaatcatct tgctcacgga taaaaatcca gttgaggtct cgcaatatgg gggtcacatc
     2341 ctgcacaacc acatcacgag ctactacagg gatgatacgt tttgaatttt tacgagcatg
     2401 ttccaattcg accttacaga cttctgattt gatcgaatcc ggactgatga gaaatacaaa
     2461 aacttccgcc tgctcaatgc catgcagaat ctggtccagc cagccaaccg cagggggaat
     2521 atcctcccag tccacccaca catccagttt gacggactta aagccctcca tcaacttttt
     2581 ggcagcttga gagtcttttc gcgagtatga aataaaaata ccagtcattt aatcaacctt
     2641 aaataagaaa aaaatctaca cggaattata ccttttacaa ggtgggaaaa cctttcagct
     2701 ttttatgctg gaaggttatt aagaatttct catcaaaatc acttgggaat gtaatactta
     2761 atggtcaact gtgggcgcgc cgattgaacc gagtaattcc cgctgaaaaa cgagaggtaa
     2821 tcatcttttc ggtcatcgtc atcatccaat tgaaaaccaa ggcgaaactg agtagcccct
     2881 ttcaaattaa caagtgagaa aacgcccgag tcaaaattaa cccaatacca cccccccacg
     2941 gcattgttct gaatcgttcc ggcattatac aaactggcag gcgcttgaaa gtctgaaatc
     3001 tgcaatgcac cgatctgcaa cgggccaaaa ctaccaaatg ccccctgacg aatatccacc
     3061 cacatattgc gatgggtcgt aaagggattc gttccaacaa tagtctctag ttgcatcgtc
     3121 aatatcattt gtgtaatgat ggcattatca ggcaatgccc ctgtggagaa ctgtagaatg
     3181 gagcggtatt gattatcttg tgcattatca cctacacgga aggtgatcgc attagagttc
     3241 aaggttccac ctttattgct ggtctctttc gactccatca cccaaccatc ttgggctcca
     3301 tctgaagaga aagaaacagt ttcaatcaac gggatattgc ggtcaatcgt ataggtctcg
     3361 ccagcagcga agttcccatt ccccactcca attcctccca caggggcgcc agccgaatca
     3421 acaacactat catcgtcatt caagtcaagc cggagggttc cacttccaat acctgtgttg
     3481 acagtcacca tataggcagt gcctgaaccg cttatggatg caacgcccgc ctcactcaaa
     3541 cccgaagtgg acagcacaaa atcagccgca tccacaccgc taacggattc ggagaatgta
     3601 atgctgaagc gcacacttcc agcgttggaa gggcttgggt cagcacgcag gatggaggtc
     3661 acgaatgggg cgtttttgtt aatcacatag acattgccca atgagaaatt cccgttacca
     3721 atcccaactc cacccaaatg catagacgtt gcgctcaaga tgctgtcgtt atccacaaga
     3781 tcaagacgca gggttccatc ccctacaccg gtatacactg taatcgcata cgcgcttccg
     3841 acagggtacg catcaaggat ggtgtaattt gaaatggaac ctgttgcggt catcataaaa
     3901 tcattgacat ctacattatt gaccggctct gaaaagttca caaaaaagcg cacaagctct
     3961 gtagtatttg gtgttgggtc tgctaaagta atggaagtaa ccaccggcga cgaataaaca
     4021 ggcgtcgcgg ttggagtatc agttggcaat ggaaaaggtg tgttggtcgc gacaggtgta
     4081 gcgggagatg ttccatacac agactcatat gcgccaagat caggcgcact gcccataaat
     4141 tgatcgttaa ttccaggaat cagaacgcca cggtcaatgt tggggctgtt tgataacaaa
     4201 gtcatatctg tggataagcc cggtgaatct tcatagccat tgcaatctaa ccctgttgaa
     4261 cggcaccact gattcaaagt ggcatatgaa atgccttccc acataaaagg agcatttgaa
     4321 ttca
//
LOCUS       DAOREF010000134         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_11974_length_4323_cov_4.418463, whole genome shotgun sequence.
ACCESSION   DAOREF010000134 DAOREF010000000
VERSION     DAOREF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11974_length_4323_cov_4.418463"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKI33_03920"
     CDS             <1..472
                     /locus_tag="PKI33_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNM36181.1"
                     /translation="VATQCHSLDYLPWLVGKKVESVWGFADKLSDLEVTADDTAKIGL
                     RFEGGAVGSLHLDYNQQPPEHEFRIIGTNGTIKWNLVDGAARIYRTEKKDWDVYPLPE
                     GWERNVMFQQQTKHFVDIVNGKAQPSCTLEDGVQVQRIIASVHESQKTGKLIKL"
     gene            711..2438
                     /locus_tag="PKI33_03925"
     CDS             711..2438
                     /locus_tag="PKI33_03925"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HNM36182.1"
                     /translation="MAKAKLITPYGGKLVDLVVKGAERDALIARAGHLPSIKITMRNL
                     CDLELIATGGFSPLTTFMGKADYERVLKEMRLADGTLFPLPITLTADPKELPTVGEEL
                     ALRNANFDLIAVMRLDEVYHWDANTEAALAYGSTDTKHPMVSEMGRWGKVCISGPLQV
                     INLPKYYDFVNLRHTPAQVREMLEAMGNDNVVAFQTRNPLHRIHEELTKRAAAQVNGS
                     LIVHPVVGMTKPGDVDHYTRVRTYKALVDNHYDQKSTMLSLLPLAMRMAGPKEALLHA
                     IIRRNHGANHFIVGRDHAGPGNDSTGKPFYGPYDAQELMTKHEAELGVKMVPFEMLVY
                     LPDEERYVEEKDVPKGAKVANISGTQVRDDYLAKGKLLPEWFTRPETAEILREMYPPR
                     HKQGFGIWFTGLSGSGKSATTAVLTTLLLERGRETAILDGDVVRTHLSKGLGFSKEDR
                     DTNILRIGFVAGEIVHAGGIVICAAISPYRATREEARKMVGENFIEVYMDTPVEVCEQ
                     RDVKGLYAKARQAMADGKPMGFTGVDDPYEPPVKPEITLKGYESTPEDNARAIIKYLE
                     EQGFLLPVK"
     gene            2487..4037
                     /locus_tag="PKI33_03930"
     CDS             2487..4037
                     /locus_tag="PKI33_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNM36183.1"
                     /translation="MTKSHSASFLILGLLALSGTFLILYATPQGMGLFDDSIAYIAGA
                     RSIVAGQGYREAWLASNQPVTHFPPGFSTVLALVGISGLDPLRGTRLVNAVVFGANIF
                     LLGLIGWRMTKSKLAGMALSALLLLSGELFRVHTTAMSEPLYIFFTLAAFLAFSQTFV
                     SEEKQTNKFLLLASALTAFAYLTRYAGLALFATFLVSLFLLHPTWKQRLTSAGIFLAG
                     FVPFALAWAVRNRLLTDNATNRKLVYHPITPENIQLGISNFASFILPIEEWRKVLIKV
                     PNLVAVILIVLILALLGWVVLKGLQKFFNPASPTPEVLSFVSTLYVFGYLASILSSMT
                     LFDASTKFQLRILAPVYVSLLIMLVYFGYWLWQKQKIALILLSLVAFSFSVSSFLSTF
                     TQLRKGGQGYASFQWFDSEAMKFMQNLPAETRIYSNETAAVYLYTDHPGYVLPDLIDP
                     VTGLPRGRFDEGVKALQEDVLSGNAVLALFDVDSQDEDVQSTYQILANGLYLAFDKQG
                     DKIYTAYP"
     gene            4049..>4323
                     /locus_tag="PKI33_03935"
     CDS             4049..>4323
                     /locus_tag="PKI33_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM36184.1"
                     /translation="MSLTQKGIQMTIFDKFSLKDRVAVVTGGGGQLGFEFCKTLAEAG
                     AAVVAADLNMDFAGKTATRLTEAGYAAMAFPLDVTRPESTRELVAETV"
BASE COUNT          991 a         1062 c         1100 g         1170 t
ORIGIN      
        1 ggttgcaaca caatgtcact cattggatta tttgccgtgg ttggttggaa agaaggttga
       61 gtctgtttgg ggctttgctg ataagcttag cgaccttgaa gtgactgcgg atgacacagc
      121 caagatcggg ctgcgatttg aagggggcgc agtaggaagc cttcacctag actataacca
      181 acagccgcca gagcatgaat tccgcattat tggcacgaat ggaaccatta agtggaattt
      241 ggtggatgga gcggcgagaa tttaccgcac agagaaaaaa gattgggatg tgtacccgct
      301 accggagggg tgggagcgga acgtcatgtt ccaacagcaa acgaaacatt tcgtggatat
      361 agtcaacggc aaggcacagc cttcttgtac acttgaagat ggggtccaag tgcagaggat
      421 aattgcttcg gtgcatgagt ctcagaagac agggaagttg ataaagttat aggtgagaaa
      481 gcggcagaga acctgtcgct tttttgttgg attcgctttc agggatggga aagtttggaa
      541 ggtgtggcag gctcgaggtc cagaaagagg cggtgggttt ccaaaattca cccactgaat
      601 tttggagtat aattggcgct tgtgcaaact ggtatgggcg atggatttcc atgcatcccc
      661 atactttatg cccacgggca ttatctaaaa caaatggagt aacaatcaca atggctaaag
      721 caaaacttat taccccttac ggtggaaaat tagtggactt ggtcgtgaag ggtgccgaac
      781 gcgatgcatt aattgcccgt gctgggcatc tgccttccat caagatcacc atgcgtaact
      841 tgtgcgacct tgaattgatc gctacgggtg gtttttcgcc tttgacgact ttcatgggca
      901 aagcggacta cgaacgtgtg ctcaaagaaa tgcgccttgc agatggaacg ctctttccgc
      961 tgccgattac attgactgcc gacccgaaag aattgccgac cgtgggtgaa gaactcgcgc
     1021 ttcgcaatgc aaactttgac ttgatcgctg tgatgcgcct tgatgaggtc tatcactggg
     1081 atgcgaatac cgaagcggct ttggcatacg gctcgaccga taccaagcac ccgatggttt
     1141 ctgaaatggg acgttgggga aaagtgtgta tctctggtcc tttgcaggtg atcaaccttc
     1201 ccaagtatta tgactttgta aatctgcgcc acaccccggc tcaagtgcgc gaaatgcttg
     1261 aagcgatggg taacgataat gtggtcgctt tccaaactcg caatccttta caccgcatcc
     1321 atgaagaatt gaccaaacgt gctgcggctc aggttaacgg ttctttgatc gttcaccctg
     1381 tggtgggtat gaccaagccc ggcgatgtgg atcactacac ccgcgtccgc acgtacaagg
     1441 cattggttga caatcactac gatcagaaga gcaccatgct tagcttgttg ccgctcgcca
     1501 tgcgcatggc gggtccaaag gaagcgttac tgcatgccat tatccgccgc aatcatggcg
     1561 cgaatcactt catcgttggg cgcgatcatg cgggccctgg caatgactcg acgggcaaac
     1621 cgttctacgg tccttacgat gcgcaggaat tgatgaccaa acatgaagcg gaactcggcg
     1681 tgaagatggt tccgttcgaa atgcttgtgt atctccctga tgaagaacgc tatgtggaag
     1741 agaaagatgt tcccaagggt gccaaagttg ccaacatctc tggcacacag gtgcgtgatg
     1801 attatcttgc caagggcaaa ctgctccccg agtggttcac ccgccctgaa accgctgaaa
     1861 ttttgcgcga aatgtatcct ccgcgccaca agcagggctt cggcatttgg ttcactggtt
     1921 tgagcggctc tggcaaatct gccaccaccg ctgtcctcac caccctgttg ctcgaacgcg
     1981 gacgtgagac cgccatcctt gatggggatg tagttcgcac ccatctgtcc aagggacttg
     2041 gttttagcaa ggaagaccgc gataccaaca ttctccgcat tggcttcgtg gctggcgaga
     2101 tcgtgcacgc tggtggcatc gtcatctgcg ccgctatcag tccgtaccgc gccacccgcg
     2161 aagaagctcg caagatggtc ggtgaaaact tcatcgaagt ttacatggac actcccgttg
     2221 aagtttgtga acagcgcgat gtgaagggtt tgtatgccaa ggctcgtcag gcgatggcag
     2281 atggcaagcc gatgggcttc acgggcgttg atgatccgta tgaaccgcct gtcaagcccg
     2341 agatcacact caaaggctat gaatccacgc ccgaagacaa tgcgcgtgcc atcatcaagt
     2401 atttggaaga acaaggcttc ctgcttccgg tgaagtaatt gctattcagg tgacagtcac
     2461 cattatggtg actgtcacct cgttgtatga ccaaatccca ctccgcttca ttcttaatcc
     2521 tcggtctgct tgctctttca gggacctttc tcatcctgta tgcgactcca caaggcatgg
     2581 gactattcga tgattccatt gcttatattg ccggagcgag aagcattgta gcggggcaag
     2641 gctatcgtga agcgtggctt gcttctaatc aaccagttac acacttccct ccaggttttt
     2701 caactgtttt agccctcgtc ggcatcagcg gacttgaccc tttacgtggc acacgcttgg
     2761 tcaatgctgt tgtgtttggc gcaaatatct ttttgcttgg gctgattggt tggcgcatga
     2821 ccaagtcgaa attggctggg atggctttgt ctgctttgct tttgttaagc ggggaacttt
     2881 tccgcgttca taccacggca atgagcgagc cgctgtatat cttttttaca ctggcggcat
     2941 ttctagcctt ctctcaaacg tttgtctctg aagagaaaca gacaaacaaa tttttgttac
     3001 tggcgtctgc tcttaccgct tttgcttacc taacccggta tgccggtctt gccctctttg
     3061 cgacctttct cgtttcactc ttccttcttc atcccacttg gaagcaacgc ctaactagcg
     3121 caggtatttt cctcgcaggc tttgttcctt ttgcgcttgc ttgggcggtt cgtaatcgct
     3181 tactgaccga caatgccacc aaccgcaagc tggtctatca cccgatcact cctgaaaata
     3241 ttcagcttgg catttccaat ttcgcctctt ttattttgcc cattgaagaa tggcgtaagg
     3301 ttctgatcaa agttccaaat ctggtcgctg ttattcttat tgttcttatt ttggctttat
     3361 tgggttgggt tgtgctaaaa gggttgcaaa aattttttaa cccggcttct ccaacccctg
     3421 aagttctttc ctttgtcagc acgctttatg tttttggcta tcttgcatcc atcctttctt
     3481 cgatgacatt gttcgatgcc agcaccaaat tccaactccg catccttgcg ccagtgtacg
     3541 tcagcctgct cattatgttg gtctattttg ggtactggct ttggcaaaaa caaaaaatag
     3601 ccctcattct cttgtcactg gtcgccttct ctttttctgt ttctagtttt ttgagtacct
     3661 tcacccaact ccgcaaaggc gggcagggat atgcttcctt ccagtggttc gactcagaag
     3721 cgatgaagtt catgcaaaat cttcctgcag agacgcgcat ctattcgaat gaaacggcgg
     3781 cggtatatct ttatacggat caccctgggt acgtcttgcc cgatctcatt gacccggtca
     3841 ccggtttgcc gcgtgggcgt tttgacgaag gagtcaaagc attgcaagaa gatgttttat
     3901 ctggaaatgc cgtgctggcg ctgttcgatg ttgattcgca ggatgaggat gtacaatcaa
     3961 cgtatcaaat tctcgccaat ggtttgtatc tcgcttttga caaacagggc gataaaatct
     4021 ataccgcata tccgtaaggt gacatacgat gtcccttaca caaaaaggaa ttcagatgac
     4081 tattttcgat aaatttagtt tgaaagaccg tgtggctgtt gtcactgggg gcggtggaca
     4141 acttggtttt gaattttgca agacccttgc tgaagccgga gccgctgtgg ttgcggcaga
     4201 cctcaatatg gattttgctg ggaaaaccgc gacccgcctc accgaggctg ggtacgccgc
     4261 catggcattt cccctcgatg tgacccgccc cgaatcgacc cgtgagttgg ttgctgaaac
     4321 cgt
//
LOCUS       DAOREF010000135         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12035_length_4312_cov_3.288466, whole genome shotgun sequence.
ACCESSION   DAOREF010000135 DAOREF010000000
VERSION     DAOREF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12035_length_4312_cov_3.288466"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKI33_03940"
     CDS             <1..643
                     /locus_tag="PKI33_03940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM36185.1"
                     /translation="GNNLPEVVAFPPTDRFGEALSEEEIKERSGKKELNILFLGNVIE
                     RKGLLTLLQAVNRLPVTSYQVDIVGSLTSDPTYAKQMQNFVTTNNLTSHVTFHSSLNN
                     QPLIEKLKSAHVLVVPSSYEGFGIVYLEGMGFGLPAIGTTLGAASEVIDDGATGFLIQ
                     PGDAHRLAEKLQLLSENRDLLLKMSLAARSRYQRQPKWNQTANQIRDFLLSFI"
     gene            677..1270
                     /locus_tag="PKI33_03945"
     CDS             677..1270
                     /locus_tag="PKI33_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNM36186.1"
                     /translation="MTVYSYQINGMSVQTGDIICTMNGKPDILPGEFWRLVGRLVPGD
                     VDHVAIYLGPDGRCAEAGAKGVITFDVPNGIWNTERMARQRGLLFDTFYGVVSPLDVL
                     GYMEEDEFHLRKAVAQYILAQVGKPYNLNFFNSETEEAFYCSQLAYKAYQTIGINLNT
                     GLAMEQLPGTNQIVYPQEIWNGFSHHKINLFRAAVSA"
     gene            1357..2223
                     /locus_tag="PKI33_03950"
     CDS             1357..2223
                     /locus_tag="PKI33_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNM36187.1"
                     /translation="MRRLTGIILIAISAASFGTLAIFGRYAYAENIDTFTLLFLRFGF
                     SASFMTMMLILRKEPFPRGRILAQLIGMGGIGYAGQSFLYLTAIKYASAGLVALLLYL
                     YPFFVAILSMIFLQEKLTRVKAFALALALCGTALTVGPVSGQPIGAIMAIVAALGYSI
                     YIIVGANVMKHVSPFQSSTVIFTSASVVYGLLTFTNGIHLPQTNFGWLVIAGIVVVST
                     IIPVTTFLAGLERIGPTNAAMLSTIEPIITVFLATVLFGDKLAPIVLAGGALILIAVV
                     ILTRSEMRSIET"
     gene            2294..>2720
                     /locus_tag="PKI33_03955"
     CDS             2294..>2720
                     /locus_tag="PKI33_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNM36188.1"
                     /translation="MNFNNFNLDPRLKQGIMKAGYETPTPIQEAAIPAAIRGRDIIGT
                     AQTGTGKTAAFVLPILNKLLNGQRNTPRALIVTPTRELAEQINDVIKTLSAGTKLKSA
                     TIYGGVGANPQIQALRNGAEILVACPGRLLDLIAQGHAKM"
     assembly_gap    2721..2820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2821..3699
                     /locus_tag="PKI33_03960"
     CDS             <2821..3699
                     /locus_tag="PKI33_03960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNM36189.1"
                     /translation="VACPGRLLDLIAQGHAKMANIEILVLDEADRMFDMGFLPDVRRI
                     VKAVPEKRQTMMFSATFPPDVELLAKQALREPQKIAMGIVKPAHTVEHALYPVPQHLK
                     TKLLIELLKRTDTNSVIIFTRTKHRAHRVSEQIKKSGFKATSLHGDRTQGQRQAAIKG
                     FKSGTHDIMVATDIAARGLDIETVSHVINFDMPDTADAYIHRIGRTGRAQRTGDAFTL
                     VTDEDKDMIRTLEKIMGQPLKRETLEDFDYTAPAPPRTDSGGRGRGAPREGQRPPKPA
                     PTPKSYGRNRLAPRKK"
     gene            3757..4239
                     /locus_tag="PKI33_03965"
     CDS             3757..4239
                     /locus_tag="PKI33_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM36190.1"
                     /translation="MKKQNILFFLILTIIIIVFTMWLQAENRLRNSMLYFEYKTEYGD
                     TCERIAQTFNVPIDSISEINNLPSNCTFIRPNRILNIPYQSITPGESVVTTRCPDIDC
                     VTETYIVKKDETLLDIAQKFQTSEEVIINYNGLTNGVFEGMKLTIPVGDCNPFVCTNQ
                     "
BASE COUNT         1134 a         1042 c          963 g         1073 t
ORIGIN      
        1 agggaataat ctgccagagg tagtggcatt cccgccaacg gatcggttcg gggaggcgct
       61 ttcggaagaa gagataaaag aaagaagtgg gaagaaagaa ctgaatattc tttttcttgg
      121 gaacgtgata gagcgtaagg ggttgcttac acttcttcag gcagtaaacc ggttaccagt
      181 gaccagttac caggtagata tcgttggctc gctcaccagt gacccaacct atgccaaaca
      241 gatgcaaaac tttgtcacga ccaacaacct aacttcacac gtcacgtttc actcatcact
      301 aaataatcaa cccctcatcg agaaactcaa atccgcccac gttcttgtgg tgccatccag
      361 ctatgaaggc ttcggcattg tctacctcga agggatgggc ttcggtttgc ccgcaattgg
      421 caccacgtta ggtgcagcgt cagaagtgat tgacgatgga gcgactgggt ttctcatcca
      481 accgggagac gctcacaggt tggcagaaaa actgcaactc ctgagcgaga accgtgatct
      541 cctcctaaaa atgagccttg cagcgcggag tcgctatcag cgccagccca agtggaatca
      601 gaccgcgaat cagattcgag attttctact ttcatttata tagaaacttt tgctgtacac
      661 cttcgtataa agtaacatga cggtttacag ttatcagatc aacggtatgt ctgtgcaaac
      721 aggcgatatt atctgcacca tgaacggcaa gcccgatatc ttaccgggtg agttttggcg
      781 cttggttggg cggctcgtgc cgggcgatgt ggaccatgtc gccatctacc tcggacccga
      841 cgggcgctgc gccgaggctg gggcaaaagg tgtgatcact tttgatgtac ccaacggaat
      901 ttggaacacc gaacgtatgg cacgccaacg cggtttgttg ttcgacacct tttacggtgt
      961 ggtctctcca ttggatgtgc ttgggtatat ggaagaagac gaatttcatt tacgcaaagc
     1021 tgttgcccaa tatatccttg cgcaagttgg taagccctac aatctaaact tctttaactc
     1081 cgaaaccgaa gaggcttttt attgtagcca gctcgcatac aaagcctatc aaaccatcgg
     1141 cattaacttg aacacgggac tcgccatgga gcaactgcct ggcacaaatc aaatcgttta
     1201 tccgcaagag atttggaatg ggttttcaca tcacaaaata aatctgtttc gagccgcggt
     1261 ttcagcataa actccccttc aacggggagt ttttttcggg tatatactgc atttaaaaag
     1321 cacaatttac gattgagtgc ggtatagttg cccgctatga gaagactcac cggaatcatt
     1381 ttgatcgcca tttctgccgc atcatttggg acgcttgcca tctttgggcg ctatgcctac
     1441 gctgaaaaca tagacacctt caccctcctt tttctccgtt ttggattttc cgcctcattc
     1501 atgaccatga tgttgattct gcgcaaggag cctttcccac gcggacgcat cctcgcccaa
     1561 ctaatcggca tgggtggcat tgggtatgca gggcaatcgt ttctatatct aaccgctatc
     1621 aaatatgctt ctgccggttt ggtcgccttg cttttgtatc tgtatccctt ttttgtcgcc
     1681 atcttatcga tgatcttctt gcaagagaag ctcacccgcg taaaagcctt tgcgcttgcc
     1741 ttggcattat gcggcaccgc actaacggtc ggtcccgtca gtggacagcc cattggcgca
     1801 attatggcaa ttgttgccgc attggggtat tccatttaca tcatcgtcgg tgccaatgtg
     1861 atgaaacatg tcagcccatt tcagtcgtcg accgttattt tcacttccgc gagcgtggtc
     1921 tatggactgt tgactttcac caacggaatt catcttccac agacgaactt cggttggctg
     1981 gtaattgcgg gcattgttgt cgtgtccacc attatcccag tcacaacttt tcttgctggt
     2041 ttggaacgga tcggtcctac caatgcggca atgctctcga ctattgaacc catcatcacg
     2101 gtttttctcg caacggtctt atttggcgac aaacttgcac cgatcgtatt ggctggcgga
     2161 gcactgattc tcattgcagt tgtgattctt acacgctcag aaatgcgttc aatcgaaaca
     2221 taagcgatat aatgcttcat acaaagaatc aggggacgat cacgtatagt gactgtcacc
     2281 taaaaaggaa atcatgaact tcaacaattt taatctcgac ccccgcctca agcagggaat
     2341 tatgaaggcg gggtatgaga cccccacccc cattcaagaa gctgccatcc cagccgccat
     2401 tcgcggacgt gacattattg gtacagcaca aactggcacc ggcaaaactg cagcctttgt
     2461 attgcctatt ctcaacaaat tgctcaatgg tcaacgcaac acgccgcgtg cactcatcgt
     2521 cacaccgacg cgtgaattag cagagcagat caacgatgtc atcaaaacgt tatccgcggg
     2581 caccaagctg aagagcgcaa ccatctatgg cggagtcgga gcgaacccac aaatccaagc
     2641 cttgcgcaac ggggcagaaa tcctagtggc ttgcccgggg cgtctgctgg acctcatcgc
     2701 acaaggtcat gccaagatgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 gtggcttgcc cggggcgtct gctggacctc atcgcacaag gtcatgccaa gatggcaaat
     2881 atcgaaattc tcgtgctcga tgaagccgac cgcatgttcg acatgggctt tctgcccgat
     2941 gtgcgccgaa ttgtgaaagc ggtgcccgaa aaacgacaga cgatgatgtt ctctgccacc
     3001 ttcccgcctg atgtagagtt gctggcaaag caagctttaa gagaaccgca aaaaatcgcg
     3061 atgggcattg tcaaaccggc gcatacggtg gaacatgcgc tttaccctgt gcctcaacat
     3121 ttgaaaacaa aattgctgat cgaattactc aagcgtaccg acacgaattc tgtgattatc
     3181 tttacacgca ccaaacatcg tgctcatcgt gtatcagagc aaatcaagaa atctggcttc
     3241 aaagccacca gcctgcacgg tgaccgaact caagggcaac ggcaggctgc catcaaaggc
     3301 ttcaaaagcg gaacccacga catcatggtt gcaactgata ttgctgcccg cggtttggac
     3361 attgaaacgg tctcacatgt catcaacttt gacatgcccg acaccgccga tgcctacatc
     3421 caccgcattg gtcgcaccgg tcgtgcgcaa cgaaccggcg atgccttcac tttggtgacc
     3481 gatgaagaca aggatatgat ccgtacgctt gaaaaaatta tggggcaacc ccttaagcgt
     3541 gagacgcttg aagattttga ttacacggct cctgccccac cacgcactga ttctggtgga
     3601 agaggtagag gcgcccccag agaaggtcaa cgcccgccca agcctgcgcc aacgccgaag
     3661 agttatggca gaaatcgatt ggctccaagg aagaaataag tttctaaatc ataaataacc
     3721 ctgctctagc agggttattt aaaaagggag ataagcttga aaaagcagaa tattttattt
     3781 ttcttaatcc ttacaataat catcattgta ttcactatgt ggttacaagc agagaatcga
     3841 ttaagaaata gtatgcttta ctttgaatac aaaacagaat atggcgatac ctgtgaacga
     3901 atagcacaaa cttttaatgt gccaattgat tcgatttctg aaataaataa tttaccgtcc
     3961 aactgtacgt ttataagacc taaccgaatt cttaatatcc catatcaaag tattacacca
     4021 ggagagagtg ttgttacaac acggtgtcca gatatagatt gtgtaacaga aacttatatt
     4081 gtaaagaagg atgaaacact tttagatatt gctcaaaaat ttcaaacctc agaagaggtg
     4141 attattaatt acaatggatt aactaatggt gtttttgaag gaatgaagct aaccattcca
     4201 gtcggtgatt gtaacccctt tgtatgtact aatcagtaat tataggaatc ctcgaatcaa
     4261 aagagccgga tgctttcgca tccggctctt tttgtctact agctcaagat gg
//
LOCUS       DAOREF010000136         4298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12100_length_4298_cov_4.254066, whole genome shotgun sequence.
ACCESSION   DAOREF010000136 DAOREF010000000
VERSION     DAOREF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12100_length_4298_cov_4.254066"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PKI33_03970"
     CDS             <1..1230
                     /locus_tag="PKI33_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNM36191.1"
                     /translation="GASVITPTSSTPTGTLSDVTRLAKEGKLDEAARIYSKITGLSHE
                     NAMFSVKSMAGVREEEPASSNASPYSNPYGMPQASPSYQAPPVPQPVYQPKVRARGGS
                     CLSGIIRLIVLLSILGSAFPAIWKALQFQLPFELPFSTDGITIIPEPFAKEVMSINTG
                     SFKDPRAIQVDGNDNILLFNYNSSDIQMFDPQGNELSVMKITDADGDEINNSKMGVTS
                     NGTIYVPGFQSIMSFSESGERLREIPNTDQFFIIHTLLVGADDKLYVRSSSGILRFDE
                     NNKVDLFIPDATLEDLSGQTPSIGALGVDAQGNIYFSGTFNKDVLKFSPTGEFISSFG
                     GDEFTSVRAIEIDSYGRIYIVDFSEVRVYDSNFTYLGSIDGAFWGVDFDSQGYMYAVE
                     TNGDDVLKFEVKKPDAQ"
     gene            1363..2442
                     /locus_tag="PKI33_03975"
     CDS             1363..2442
                     /locus_tag="PKI33_03975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNM36192.1"
                     /translation="MSQPLNNQTRPFELGFYNFVDSGANPLTGEKKDPATVMKNMLEV
                     IELADQVGLDVFAIGEHHRVEYLASAPEVILGAAAARTKNIRLSTAVTVLSSDDPVRV
                     FQRFATVDLISNGRAEIMAGRGSFIESFPLFGYNLNDYDGLFDEKLRLLSKLNESTNI
                     TWSGNFRAPLNDLGIYPRPVQEKLPIWVAVGGTPESVVRAASMGLPMALAIIGGMPER
                     FAPYLDLYRQASAEYGFEPDTIPLSINSHGFIADTAEEAVKEYYPIQTYNMNKIGRER
                     GWAPATQDQLEKQRHLRGSDFVGTPDEIIEKILFQYDIFKHQRFLMYMGNNAIEHKKM
                     MHAIELFGTKVAPTVRKEIEKRRAG"
     gene            2602..3369
                     /locus_tag="PKI33_03980"
     CDS             2602..3369
                     /locus_tag="PKI33_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNM36193.1"
                     /translation="MTTSDVVSAETLETKTKLNGKILKTTLAGALVDIGQSLPGVLHI
                     SQISETPVNKVEDVLKEGQEVTVWVKRVRKDRIELTMIEPLMYDWKELKPDMVVKGKV
                     TRLETYGAFVDFGAERPGLIHVSELAHGYVKTAGDVVKAGDEVEAKVLEVDRRKRQIR
                     LSIKALQELAVEEAAEPVEYKGKGKRKGKKQEESYEMETEVSNEPQYTAMQIAWQQAL
                     DKANGKKNIRAKSIKGTSKEQENLYNSTLEKRLPTGG"
     gene            complement(3419..>4298)
                     /gene="prfB"
                     /locus_tag="PKI33_03985"
     CDS             complement(3419..>4298)
                     /gene="prfB"
                     /locus_tag="PKI33_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNM36194.1"
                     /translation="KQQVHDLTDLASLEDETLRAELETEIQKVEKELEKRSFTMMLSG
                     PFDHDDAILAIHAGAGGTDSQDWAGMLQRMYLRWIESRGFTAEILDMTPGEEAGIKSM
                     TIAVSGQYAFGYMRSEKGVHRLVRLSPFDAAHRRHTSFALVEVLPQVSMDEADVEINQ
                     SDVKMDVFRSSGAGGQNVQKNATAVRLTHIPTGIVVTCQNERSQTQNREFAMRILRAR
                     LLQLKQAEKEEERAVLRGEYTKAEWGSQIRSYVLHPYQMVKDHRTDFQVGNAQSVLDG
                     DLDAFMEDYLKSGDGK"
BASE COUNT         1142 a         1150 c          975 g         1031 t
ORIGIN      
        1 ggggcatctg tcatcacccc aacttcatcc acaccaacgg gcactctctc agatgtcacc
       61 cgcctcgcca aagaaggcaa gctggatgaa gccgcccgca tctacagcaa gatcacaggg
      121 ttgagccacg aaaacgccat gttcagcgtc aagtctatgg ctggggttcg ggaggaagag
      181 cccgcttcga gcaacgccag cccatattca aatccatacg gtatgccgca ggcaagtccc
      241 agctatcagg cgccgccagt gccccagcct gtgtatcaac ccaaagtccg cgcaagaggc
      301 ggctcttgtc tatctggcat tattcggttg atagtcctac tctccatttt gggaagcgcc
      361 ttccctgcca tttggaaagc gctacaattt cagcttccgt ttgaattgcc attctctaca
      421 gatggcatca ccatcatccc tgaacctttt gcaaaagaag tcatgtcgat taacacgggt
      481 tcttttaaag acccacgcgc cattcaagtg gacgggaatg acaatattct gttgttcaat
      541 tacaacagtt ccgatattca aatgttcgac ccacaaggca atgaactatc cgtcatgaag
      601 atcaccgatg ctgacgggga tgaaatcaac aacagcaaaa tgggcgtcac cagcaacggc
      661 acgatctatg tacccggttt tcaaagcatt atgagcttta gcgaaagcgg agaacgcctt
      721 agagaaattc caaataccga ccagttcttt atcattcata ctttattggt cggcgccgat
      781 gataagttat acgtccgttc tagttcaggt attctgcgct ttgatgaaaa taacaaggtc
      841 gatttgttca tcccagatgc aaccctcgaa gacctatcag gtcaaactcc cagtattggc
      901 gctttgggtg tagatgcgca aggaaatatt tactttagcg gcaccttcaa caaagatgtg
      961 cttaaattct ctcctacggg tgaatttatc agcagctttg gaggtgatga gttcaccagt
     1021 gtgcgcgcaa ttgaaatcga ctcctatggt cgtatctata ttgtcgattt ctctgaagta
     1081 cgagtgtacg attcgaattt cacctacctt ggcagtatcg atggcgcatt ctggggtgtt
     1141 gactttgact cacaaggcta catgtatgcg gtcgaaacga acggcgacga tgtcttgaaa
     1201 tttgaagtca aaaagcccga tgcgcaatag ccctaatttc tgcaatttat aattctcaaa
     1261 gcgaccctct tctacgaaaa agggtcgctt tttagtaaga ctttcgcctg tagctgcttc
     1321 tgtgtctcta cagaaaccat taatattgaa tcggaaatca aaatgtctca accattaaat
     1381 aaccaaactc gcccatttga attaggcttt tataacttcg ttgatagtgg cgcaaatccg
     1441 ctgaccggtg aaaagaaaga ccccgccaca gtgatgaaaa atatgctcga agtcatcgag
     1501 cttgccgacc aagttggttt ggatgtattc gccattggcg aacatcaccg cgtggaatat
     1561 ctcgcctccg ccccagaggt catcctcggt gctgccgccg cgcggacgaa gaacatccgc
     1621 ctctctaccg ccgtaaccgt actcagctcc gatgaccccg tgcgcgtctt tcagcgcttc
     1681 gccacagtcg accttatttc caatggtcgc gccgaaatca tggctgggcg cggctctttc
     1741 attgagtcct ttccgctctt tggctacaac ctcaacgatt acgacggtct tttcgatgaa
     1801 aagttacgct tgctctcgaa gctcaatgaa tccaccaata ttacatggtc tggcaacttc
     1861 cgcgcaccgc tgaatgacct cggcatctat ccgcgcccgg tgcaagaaaa gctcccaatc
     1921 tgggtcgcgg ttggcggcac ccctgagtcg gttgtccgcg cggcttcgat gggtctgccc
     1981 atggcgcttg ccatcattgg cggcatgccc gaacgcttcg ctccctacct cgacctttac
     2041 cgtcaggcat ctgccgaata tggttttgaa ccagatacca ttccattgag catcaactca
     2101 cacggtttta tcgctgatac cgccgaagaa gccgtcaaag aatattatcc aattcaaact
     2161 tacaacatga acaagatcgg gcgcgagcgc ggctgggcgc ctgccacgca agatcagctt
     2221 gaaaagcaac gtcacctgcg cggctcagac tttgtcggca cgccagatga gatcatcgag
     2281 aagattctct tccaatacga catcttcaaa catcaacgct tcctcatgta tatgggcaac
     2341 aatgctatcg agcacaaaaa aatgatgcat gccatcgagc ttttcgggac gaaagtcgcg
     2401 ccgacagtga gaaaagagat cgaaaaacgc cgagcaggat aaaggtccgc ttcgaaccgc
     2461 gctctttcct gattctcctg ctcaaacatg acaatacgcc gagagattct catccctcac
     2521 taaccttttt actcattgac gcatcgtttt cagaaaggta taatcgttct tcggcattat
     2581 ttcaaacagc aggagctaat tatgactaca tcagatgtcg tgtccgctga gacactcgaa
     2641 accaaaacaa aattgaacgg aaaaatcttg aagaccacac tcgcaggggc gttggtggat
     2701 attggacaga gcctccctgg cgtgctccac atctcacaga tcagcgaaac cccggttaac
     2761 aaagtggaag atgtcctcaa ggaaggtcag gaagtgaccg tgtgggtcaa gcgtgttcgc
     2821 aaggatcgca ttgaactcac catgatcgaa ccgcttatgt acgattggaa agaactcaag
     2881 cccgatatgg ttgtgaaagg caaagtaacc cgcctcgaaa cctacggcgc ttttgtagac
     2941 ttcggcgcag aacgccccgg cttgattcac gttagcgaat tggctcacgg ctatgtcaag
     3001 actgctggcg acgttgtgaa agctggcgat gaagtggaag ccaaggttct cgaagtagat
     3061 cgccgcaagc gccagatccg cttgagcatc aaggctcttc aggaactcgc ggttgaagaa
     3121 gccgccgaac ccgttgagta caagggcaaa ggtaagcgca agggcaagaa acaagaagaa
     3181 tcctacgaaa tggaaacgga agtttccaac gaaccgcagt acactgccat gcaaatcgct
     3241 tggcagcaag cgttggacaa ggcaaacggc aagaagaaca tccgcgccaa atctatcaag
     3301 ggtacttcca aagagcagga aaatctttac aacagcacct tagaaaaacg cctccccact
     3361 ggcggctaaa gaaaaacaaa aaagagcgga caaatgtccg ctctttttat gtaccaattt
     3421 acttcccatc cccactcttc aaatagtctt ccataaacgc atccaaatcg ccatctagca
     3481 cagactgcgc attgccaacc tgaaaatcgg tgcgatggtc tttcaccatt tggtatggat
     3541 gcaagacata cgaacgaatt tggctgcccc attctgcctt ggtgtattct ccgcgcaaca
     3601 cagcgcgttc ttcttctttc tctgcttgct ttaactgaag caaacgcgca cggagaattc
     3661 gcatggcaaa ctcgcggttc tgcgtctgtg aacgttcatt ctgacaagtc actacaattc
     3721 ctgttggaat atgtgttaag cgcacagcgg tggcattttt ctgcacattc tgtccgcctg
     3781 cacccgagga acggaagaca tccattttta catcgctctg gttgatctcc acatctgcct
     3841 cgtccatgct gacttgcggc aacacttcaa ccaacgcaaa ggatgtatgt cggcgatgag
     3901 ctgcgtcaaa aggagaaagc cgcaccaggc gatgaactcc cttttcagaa cgcatatacc
     3961 cgaaagcata ttgtccgctt acagcgatgg tcatgctttt gattcctgct tcttcaccgg
     4021 gagtcatatc taaaatttcg gcggtgaagc cacggctttc gatccaacgc agatacatac
     4081 gttgaagcat cccagcccaa tcttgtgagt ctgttccacc agcgccagca tgaattgcga
     4141 gaatcgcatc gtcatggtca aacggacccg aaagcatcat ggtgaaagag cgcttttcca
     4201 gttctttctc aaccttctga atttcggtct ccaactctgc acgaagagtc tcgtcctcca
     4261 gtgaggcaag gtctgtcaaa tcatgaactt gctgcttg
//
LOCUS       DAOREF010000137         4292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12128_length_4292_cov_4.484541, whole genome shotgun sequence.
ACCESSION   DAOREF010000137 DAOREF010000000
VERSION     DAOREF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12128_length_4292_cov_4.484541"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PKI33_03990"
     CDS             complement(<1..1260)
                     /locus_tag="PKI33_03990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNM36195.1"
                     /translation="MFTSQTFASILPEILILTIGLLVLIFEPFWKGEQKRNAGWLTAG
                     GLLVAMLVSLIFARPGEPVAVLGGMLRFDWLGFFLKMLFMFAAAATALLLMDHEKVGK
                     RGEAYLMLLASLIGMNFMASAGDLIMLYLSIETASIPLYILSGFLLDDEHSTEAGFKY
                     FLYGAMSSTIMLYGFSLVFGFSGTTDLYAIATSLTSGSISPLIAFGVVALVVVGLGFK
                     VSIVPFHFWAPDVYQGAPTPVAGYLSTASKAAGFAVILRLFFVAFPEFSSTWSMILAV
                     LAAISMTVGNLLALPQTNIKRLLAYSSISHAGYAMIGAVAFSQFGAASVVFYLAAYIA
                     TNLLAFGIVMAFSRVTGLEEITDYAGLSRRSPLLGLMMLAAFLSLTGMPPFGGFVAKV
                     IVFAAGVQANYTWLVVVGIINSIIGVYY"
     gene            complement(1260..2780)
                     /locus_tag="PKI33_03995"
     CDS             complement(1260..2780)
                     /locus_tag="PKI33_03995"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNM36196.1"
                     /translation="MNFPILSVITFLPFAAGMLMLMMPANRKNEVRAVALASAVIDLL
                     LSAWVYLQYLLNHMQGYQFIEKYTWLNIPPLNIALHFGVDGMSAPLVLLTGIVMFTGV
                     LISWGNDDPHVMSGIQDRPREFFAFLFLLAGGVFGVFVSLDLFMLFFFYEIAVFPMYL
                     LIAIWGWVKTREYAAMKLTLYLFVGSVVALVGALAMYWTQYQNTGILSFDMIQMESAG
                     FSSAFQNLWFLPVFLGFAVLGGIWPFHNWSPDGHVAAPTAVSMFHAGVLMKLGAFAAL
                     RVGVMLLPEGAKHWSWLIMTFAGVAVVYGAYIAFVQTDLKYMIGFSSVSHMGLVVLGV
                     STLNETGLLGAGVQMVSHGIMTALFFAVTGMIYDRAHTRMIPELGGMAKIMPFAAIGF
                     IIGGLVSMGMPGFSGFVAEFPIFMGVWQEQWLVAVICSISIIITAAYIMINIRKVFFA
                     PLPEKLEGHVGDVTVLDKVAIVTLCLLMIAIGMFPSVMVPMVKTGVDYIMTILGGA"
     gene            complement(2791..>4292)
                     /locus_tag="PKI33_04000"
     CDS             complement(2791..>4292)
                     /locus_tag="PKI33_04000"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNM36197.1"
                     /translation="LLSLILFIPAISAVIMLFLPNEAQLQRWFAFVASLIPFVLTLVL
                     WSKFDPNATGFQFEEKYVWYEAIHSSFHIGVDGISFSMVLLTTFLTPLAILASFSVSD
                     RVKPYMMLFLFLETGMLGVFLALDLLIFFVFWEIGLVPMYFLINQWGSANRDYASLKF
                     MIYTMGGSLGLLLAVQMLGVLFGTYDLVSLYQQWNSLTADTVLMGMTVGTVKTIAFWA
                     FAIAFAVKVPLWPFHTWLPDAHTEAPTAGSMILAGVLLKLGAYGFIRLVLPLYPVEAK
                     IFAGALAVLAVGAIVFGAFGSFGQTDFKRLVAYSSVNHMGFVVLGIAAAGLAAGTADA
                     HIAMSGAVLQMFNHGLSAAGMFFLVGVIYERTHTRNLDEFGGLFPLVPVYGGILIFTS
                     MASLGLPGLNGFVSEFMIVRGSYQPLTIYTAIAMLGLLFTGAYILKGIKKVLHGPMNE
                     HWAHGEHKLTEINLREILVMVPLMALMLLTGIWPMWILDIINKAVTALF"
BASE COUNT         1380 a         1075 c         1093 g          744 t
ORIGIN      
        1 gtagtacaca ccaatgatcg agttgataat accgacaaca accaaccaag tgtaattggc
       61 ttgaacgcct gcagcaaaga caatcacctt tgccacgaaa ccaccgaagg gcggcatacc
      121 ggtcagcgag aggaaggcgg cgagcatcat caagccgagc aaggggctac ggcgactaag
      181 acctgcatag tcagtgattt cttccaaacc tgtgacacgg ctgaacgcca ttacgatacc
      241 gaaggcgagc aagttggtag caatgtaagc tgcgagataa aagaccacac ttgccgcacc
      301 aaattgacta aacgccactg ccccgatcat ggcatagccc gcatgggaaa tggaagaata
      361 agccaagagg cgcttgatat tagtctgagg cagagctagt aaattaccta ccgtcatgga
      421 aatagcagcc aacacagcaa gaatcatcga ccaagtgctg ctaaattcag ggaaggcaac
      481 aaagaacaag cgcaggatta ctgcaaaacc agccgctttt gaagccgtgg aaagataccc
      541 agctactggt gtaggagcac cctgatatac atccggtgcc cagaagtgga atggcacaat
      601 ggaaaccttg aagcccaaac cgacaacgac caaagcaact acgccaaagg caatcaacgg
      661 ggaaattgaa cctgaagtca acgaggtagc gatggcatac aagtctgtgg tgccagagaa
      721 accaaagacc aagctgaagc catacaacat aatggtggaa gacatcgcac catacaagaa
      781 gtatttgaag cccgcttccg tagagtgttc atcatccagc aagaagccgg agagaatgta
      841 aagcggaatg gacgccgtct caatggagag gtagagcatg atcaagtcac cggcagaagc
      901 catgaagttc ataccgatga gagatgcaag caacatgaga taagcttcac cacgcttgcc
      961 cactttttca tggtccatca aaagaagggc tgtggcagcg gcggcgaaca tgaagagcat
     1021 tttgaggaag aagcccagcc aatcaaagcg gagcatgcca cccaaaactg ctacgggctc
     1081 accggggcga gcaaagatca agctgacaag catagcgacc aataaaccac cagcagtcaa
     1141 ccagccggca ttgcgcttct gctcgccttt ccagaaaggc tcaaagataa gaaccaaaag
     1201 cccaatggtt agaatcagaa tttcgggaag aatggaggcg aaagtctgtg aagtaaacat
     1261 tatgcacctc ccaagatggt catgatgtaa tccacgccag ttttcaccat cggaaccatc
     1321 acggacggga acatgccgat cgcgatcatc aacagacaaa gcgtgacaat ggcgacctta
     1381 tccaagaccg tgacatcccc tacatgacct tctaattttt caggtaatgg ggcaaagaaa
     1441 actttacgga tattgatcat gatgtaggct gcggtaatga tgatggaaat agaacaaatt
     1501 acagccacca gccactgttc ttgccagaca cccatgaaga tcgggaattc agctacaaag
     1561 ccggagaaac caggcatgcc catcgagacc aagccgccga taatgaagcc aatggcagcg
     1621 aaaggcataa tctttgccat gccaccaagt tcagggatca tacgggtatg cgcccggtcg
     1681 tagatcatac cggtgacagc aaagaagaga gcggtcatga tgccgtgaga gaccatttgc
     1741 acgccagcgc caagaagacc agtttcattg agagtagaaa cgcccaaaac caccaagccc
     1801 atgtgcgaaa cggacgagaa accgatcatg tatttcaaat cagtctggac gaaagcaatg
     1861 taagcgccgt ataccactgc aacaccagcg aaggtcatga taagccaaga ccagtgtttt
     1921 gcaccttctg gcaggagcat gacgcccaca cgcaaagcag cgaatgcgcc gagtttcatc
     1981 aatacgcctg catggaacat ggacacagcc gtcggtgcag ccacgtgacc atcaggcgac
     2041 cagttatgga agggccagat accacccaaa accgcgaacc ctaaaaagac gggcaagaac
     2101 caaagatttt ggaaagcgga agagaaacca gcactttcca tttggatcat gtcgaaagaa
     2161 agaatgccag tgttctgata ttgagtccag tacatggcaa gggcaccaac aagagcaacc
     2221 accgaaccca cgaacaggta gagcgttaac ttcatggctg catattcacg ggtcttgacc
     2281 cagccccaaa tggcgatgag caagtacatg gggaacacgg caatttcgta gaagaagaac
     2341 agcatgaaca ggtcaagtga gacgaacaca ccgaacacac cgcccgccag caagaacagg
     2401 aaggcgaaga attcgcgtgg tctgtcttgg atgccagaca taacgtgggg atcgtcgttg
     2461 ccccatgaga tgagcacacc ggtgaacatg acgattccgg tcaacaaaac gagcggagcg
     2521 ctcataccgt cgacgccgaa gtgcaaagcg atattcaacg gagggatgtt gagccaggtg
     2581 tatttttcaa tgaactggta accctgcata tgattgagca ggtattgcag gtaaacccag
     2641 gcagagagca gcaaatcgat cactgccgaa gccagagcca ccgcgcggac ttcattcttc
     2701 cggttggcag gcatcatcaa catgagcatg ccggcagcaa atggaaggaa ggtgataaca
     2761 cttaggatgg gaaaattcat catccacctc ttagaacaga gccgtcacgg ctttgttgat
     2821 gatatcgagg atccacattg gccagatgcc agtgagcaac atcagcgcca taagcggaac
     2881 catcacgaga atctcgcgca ggttaatctc ggtcagtttg tgttcgccat gtgcccagtg
     2941 ttcgttcatg ggtccatgca acactttctt gatgcccttc aagatataag cacctgtaaa
     3001 gagcaagcca agcatggcaa tggcggtgta gatcgtcagc ggctgataag agcctcgcac
     3061 aatcataaat tcagagacaa agccgttcaa accgggcaaa ccaagcgaag ccatggaggt
     3121 gaagatcaag atgccgccat agactggaac caacgggaac aagccgccga attcatccaa
     3181 attgcgggtg tgggttcgtt cgtagatcac gccgacgagg aagaacatgc ctgctgctga
     3241 aagaccgtgg ttgaacatct gcaaaacagc gccactcatg gcaatgtgag catcagccgt
     3301 accagcagcg agccccgcgg cggcgatacc gagcaccaca aaacccatat ggttcacaga
     3361 agaataggcg actaaacgtt tgaagtctgt ttgaccgaag gaaccaaagg cgccgaatac
     3421 aatcgcaccc acggcgagaa cagccaaagc acctgcaaaa atcttggctt ccacaggata
     3481 aagcggcaac accaagcgga tgaaaccgta agcacccaat ttgagcaaca cacccgcgag
     3541 gatcattgaa ccagcggtgg gagcttcagt atgcgcatcg ggtaaccaag tatggaaggg
     3601 ccacaaagga actttgacag cgaaggcaat ggcaaacgcc caaaaggcga tggtctttac
     3661 agtgcccact gtcatgccca tgaggacagt gtccgctgtc agcgaattcc actgttgata
     3721 gaggctgacc aagtcatagg tcccgaaaag aacgcctaac atctgcaccg caagcaacaa
     3781 gccgagcgat ccgcccatgg tgtagatcat gaacttcagc gaagcatagt cacgattggc
     3841 acttccccac tgattgatga ggaagtacat tggcacaaga ccaatttccc agaacacaaa
     3901 gaaaatgagc aggtcgagag ccaaaaagac gccgagcatg cctgtttcaa gaaacaggaa
     3961 gagcatcata tatggcttga cgcgatcact cacgctgaac gaagcaagaa tggcaagcgg
     4021 ggtcaggaag gtggtgagca aaaccatcga gaacgaaata ccgtccacac caatatggaa
     4081 ggacgaatga atggcttcgt accagacata tttttcttcg aactgaaagc cggtggcgtt
     4141 cgggtcgaac ttgctccaca acacgagtgt aagcacaaaa ggaatcaagc tggcaacgaa
     4201 agcaaaccaa cgttgaagtt gagcttcatt cggcaaaaag agcatgatca ctgcagaaat
     4261 ggcagggatg aaaaggatca ggctaaggag at
//
LOCUS       DAOREF010000138         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12162_length_4283_cov_3.547777, whole genome shotgun sequence.
ACCESSION   DAOREF010000138 DAOREF010000000
VERSION     DAOREF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12162_length_4283_cov_3.547777"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKI33_04005"
     CDS             <1..419
                     /locus_tag="PKI33_04005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36198.1"
                     /translation="TVRPLGGAVEKYVALHQSVFESKNMTIDWRGRTLQSPLYKPELD
                     LVVESPTGELAAFCICWFNEKLSAGQVEPLGVHADYRQLGLGKVALSHGVSRLRSMGA
                     KDIFVETDNYRNEAFNLYQSIGFEVIQDVLVYRKDY"
     gene            446..634
                     /locus_tag="PKI33_04010"
     CDS             446..634
                     /locus_tag="PKI33_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HNM36199.1"
                     /translation="MTETTQIKDGDHCEVVKGTHAGKSGTVRDIKTSKTGQVTITVVQ
                     SDGERFKTLARNVVVKKR"
     gene            644..766
                     /locus_tag="PKI33_04015"
     CDS             644..766
                     /locus_tag="PKI33_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36200.1"
                     /translation="MRDEVTFIETDNYHNKVFNLYQSIGFEVIQDVLVYRKDYS"
     gene            834..1637
                     /locus_tag="PKI33_04020"
     CDS             834..1637
                     /locus_tag="PKI33_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36201.1"
                     /translation="MTILYKQALDLLDARSLVTSLDHDLTRLIRRELSASLGGSDDIA
                     DRVLKNILLRWNCALSAADDEVTARQKNLIVVTLPLLLHKYGIQDPSLNSKAYQAIEA
                     LNREVILSDSFYRKSSAIKELLSSPVALRSRKPARPADLTFWRTGDLVAYQIAGKFYA
                     LYIHGIVGANEAPIVEFMDMETSYRPVRDDVLGKKARGIPFKDGVLHVQHYLVYGMRD
                     MPDPANQFHLLGNMPQYKPDQTHLAPPIGMGVVTDVFRLADEVREKLFP"
     gene            1651..2274
                     /locus_tag="PKI33_04025"
     CDS             1651..2274
                     /locus_tag="PKI33_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36202.1"
                     /translation="MTENFIFPFELQQSRPFHFVEETSLAAARIGGTMPDGISVPLRD
                     DARYFGTFPLYDGNSRLFFSIFINGSFASFIHALNAGFQTDDRVVVIVHEKKPRSTDT
                     KYASGLSPHAIQVHDIASDLIQNDIGETVIRERHKFGGRPYSIQEPILPGSEALFAQG
                     YRQVLQIDFPNPTDGAISGSWPFGDGIFNLFWKAPFEKEPYFWCLQG"
     gene            2384..3004
                     /locus_tag="PKI33_04030"
     CDS             2384..3004
                     /locus_tag="PKI33_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNM36203.1"
                     /translation="MINPEEAVIINSLVRQALTSMQEVMGDNGLNAVLKSCGLERFVG
                     NFPPNNLEPAIKTSQYAQLNQAIEDFYGRGGKGMLQRIGRASFQYGVREQAALLGVAG
                     VALKLMPEKQRIKFILNGMADALKKSNAQVKAWVDESGERLAYVDATCGICNSRHSDH
                     PICHLYIGSVSEAVKWATGKDYEVIETHCTAKGDEYCRFEVGNVKE"
     gene            3098..4159
                     /locus_tag="PKI33_04035"
     CDS             3098..4159
                     /locus_tag="PKI33_04035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNM36204.1"
                     /translation="MNVNDLDEKRKYSLLRIFFSLSLVINAFFVVLQLYLKNTELALV
                     ALINIFVFGGNFYLLQKRKYLLAMHIGITAITIASLVSNFYLGWESYFSLYLIASTII
                     IINSVTLSLRRKLIEVTALMTLFVASFIHMKTFTPLYDLNPVLVERIGLFNSGAVLFS
                     VLFMQFHNFIENENLKKKLQEMSEIDPLTGAYNRRFFNEYLDIELKRLESPSRYAPAK
                     KTNFGIAIIDIDDFKMINDMYGHLVGDMVIAEVAGIIKSAIFKRDIFCRYGGEEFLVL
                     FTNTSKSGVKIAIEKIRTAIQNHVFMTHGERLNIEVTVSVGFAGFSEEANVYKLLDLA
                     DKRLYKVKGAGKNGAILGE"
BASE COUNT         1110 a          860 c         1090 g         1223 t
ORIGIN      
        1 ttactgtccg cccgctgggt ggggctgtag agaagtatgt ggcgttgcat cagtccgtgt
       61 ttgagagcaa gaacatgacg attgattggc gtgggcgcac gctgcaaagt ccgctctata
      121 agccggagtt ggatctcgtg gtcgagtcac cgacggggga gttggcggcg ttttgcattt
      181 gctggttcaa cgagaaactg tccgcggggc aggtcgagcc gttgggtgtg catgcggatt
      241 atcgtcaact cgggttgggg aaggtggctt tatctcatgg cgtgagtcga ctccgctcga
      301 tgggggcgaa ggatattttt gtcgagaccg acaattatcg aaatgaggcg ttcaatctgt
      361 atcagtccat tggctttgaa gtgattcagg atgtgctggt ctatcgaaaa gattattaga
      421 agtacttgta gaaggattga gtattatgac agagactaca caaattaaag acggagatca
      481 ttgcgaagtg gtcaaaggaa cgcatgcggg caaatcggga accgtccgcg atattaagac
      541 gagtaagacg ggtcaagtca ccattacggt ggtgcagtcc gatggggaac gatttaagac
      601 ccttgcgagg aatgtcgttg tcaagaaaag atagagtaaa gtcatgaggg atgaagttac
      661 ttttatcgaa actgataatt atcacaataa ggtgttcaat ctctatcagt ctattggatt
      721 tgaagtgatt caggatgtgc tggtgtatcg aaaagattat tcctaatcaa taagatggag
      781 ttaggaaatt agtctgattg accattgcat gaaattgaag aaaacttggg ggtatgacaa
      841 tactatacaa acaagccttg gatttgctcg acgcgcgctc actggtgacg tcgctcgacc
      901 atgacctgac gcgcttgatc cgcagggagt tatccgcttc gcttggcggt tctgatgata
      961 tagcggatcg ggttcttaag aatattctgc ttcggtggaa ttgtgcttta tctgccgctg
     1021 atgatgaagt gaccgcgagg caaaagaatt tgatcgtggt cacgttgccc ctcctgctcc
     1081 acaaatatgg aattcaagac ccttctctta attcaaaagc ttatcaggct attgaggcgc
     1141 ttaaccggga agtaattctc agtgattcat tctatagaaa gtcttcggcg atcaaagaac
     1201 tcctatcctc tccggttgct cttcgctcac gaaaacccgc ccgcccggca gatctcactt
     1261 tttggaggac aggagatttg gtggcgtatc aaatagcggg gaaattttat gccttgtata
     1321 tccatggcat tgtaggcgcg aatgaagcgc ctatcgtaga gttcatggat atggagacgt
     1381 cttatcgccc tgtgagggac gacgtcttgg ggaaaaaggc gcgcggtatc ccatttaaag
     1441 atggagttct tcatgtgcag cactatcttg tctatggcat gcgtgacatg cccgacccgg
     1501 cgaaccaatt ccatctgctc gggaatatgc cccaatacaa accagaccaa actcatctcg
     1561 ctccacccat cggcatgggc gtagtgacag atgttttcag gcttgcggat gaagtccgcg
     1621 agaaattatt tccttgaaaa ggaacgtatg atgaccgaaa attttatttt cccattcgaa
     1681 cttcagcaat cccgtccgtt ccattttgtc gaagagactt ctctcgcagc tgcaagaatt
     1741 ggcggtacca tgccggacgg aatatccgtc cctctccgtg acgatgcccg ctattttgga
     1801 acctttccgc tttatgatgg aaattcccgc ctcttctttt ccatttttat taatggctct
     1861 ttcgcaagct ttatccacgc cttgaatgcc ggttttcaaa ctgacgatag agttgttgtt
     1921 atcgtccatg agaagaaacc acgcagtact gatacaaaat atgcatctgg gcttagtcca
     1981 catgccattc aggtccatga tattgcttca gacctcattc agaatgatat tggtgaaaca
     2041 gtgattcgtg agcggcacaa atttggcgga cgtccataca gtattcaaga acctatcctg
     2101 ccgggaagtg aggcgttgtt tgcgcaaggc tatcggcaag ttttacagat cgattttcca
     2161 aaccctactg acggagcgat ctccggctcc tggccctttg gtgatgggat tttcaatttg
     2221 ttttggaaag ctccattcga aaaggagccc tatttttggt gtttgcaggg ataagaacgt
     2281 ctttttgaaa agtttaagat tggtactata aggtcattac acatactttc aatagtgagt
     2341 atgctaaaga aagaatcttt gatccataac gataggagcc catatgataa acccagaaga
     2401 agccgtcatt atcaactcgc tcgtgcggca ggcgttgacc tccatgcagg aggtgatggg
     2461 ggataacggt ttgaacgccg tcttgaaatc ttgcggattg gaacgttttg tagggaattt
     2521 ccctccgaac aaccttgagc ctgcgatcaa gacctcacaa tatgctcagt tgaatcaagc
     2581 cattgaagat ttttatgggc gcggcggcaa aggcatgttg cagcgcattg gcagggcatc
     2641 ctttcaatat ggggtacgag aacaggcagc tttgttgggc gttgccggtg tggcactcaa
     2701 gttaatgcct gaaaaacagc gcattaagtt cattctaaat ggcatggcag atgcgctcaa
     2761 gaagagcaat gcacaggtca aagcttgggt agatgaaagc ggtgaacggc tggcatacgt
     2821 cgatgctact tgcggaatct gtaactcccg ccatagtgac catcccattt gccatttgta
     2881 tatcgggtcc gtttctgagg cggtgaaatg ggcaaccggc aaggattatg aagtcatcga
     2941 aacacactgc actgccaaag gggatgaata ttgtcgcttc gaggtaggaa atgtgaagga
     3001 ataatttgaa aattgtaaga gttttctccg cctcttggtt ttaggggcgg ttttttatgt
     3061 ggggggatat aatcgaacaa agtttgagta tgatgatatg aatgtgaatg atttagatga
     3121 gaaaagaaaa tattctcttt taagaatatt cttttctcta agcttggtaa tcaatgcttt
     3181 ttttgttgtt cttcaacttt atttgaaaaa caccgagctg gctttggttg ccttaatcaa
     3241 tatttttgtg tttgggggta atttttacct tctccaaaag agaaagtatt tgcttgccat
     3301 gcatattggc attacggcaa tcacaatcgc ttctttagtt tccaattttt atttgggatg
     3361 ggaaagttat ttcagcctgt atctaattgc ttccaccatc attattatca attcggtaac
     3421 gctctctttg aggcggaaat tgattgaagt gaccgctctt atgacgctat tcgttgccag
     3481 ttttattcat atgaagacgt ttacgcctct ctatgacctg aacccggttc tcgtggagcg
     3541 gatcgggctg ttcaattcag gcgcggttct tttttcggtc ttgttcatgc agttccataa
     3601 ttttattgag aacgagaacc tcaagaaaaa acttcaggaa atgtctgaga tcgatccgct
     3661 gaccggggcg tataaccggc gtttcttcaa cgagtatttg gatatcgaac tcaagcgttt
     3721 ggaaagccct tcccgatacg cccctgccaa gaagacaaat tttgggatcg cgatcattga
     3781 cattgatgat ttcaagatga tcaatgacat gtatgggcat ctcgttggcg atatggtgat
     3841 cgcagaagtg gcaggtatca tcaagtccgc gatttttaag cgcgatattt tttgccgcta
     3901 tgggggcgaa gagtttttag tcttgtttac aaacacgtca aaatcgggcg tgaagatcgc
     3961 gatcgaaaag atacgaactg caattcagaa ccacgtgttt atgacccatg gcgagagatt
     4021 gaatattgaa gtcacggtca gtgtagggtt tgcgggtttc agtgaagaag ccaatgtcta
     4081 taaactgctg gatcttgcag ataagcgttt gtataaagtg aaaggggcag ggaagaatgg
     4141 cgcaatattg ggcgaatgac ttgttctgta aataaaaaat gctccagaaa atctggagca
     4201 ttttttattt gaaatcgttc tttacggggt gggtgttgca gctgcgctgt tctttgcatc
     4261 gagtgtggca acagactcag ctt
//
LOCUS       DAOREF010000139         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12167_length_4281_cov_4.596782, whole genome shotgun sequence.
ACCESSION   DAOREF010000139 DAOREF010000000
VERSION     DAOREF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12167_length_4281_cov_4.596782"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKI33_04040"
     CDS             complement(<1..203)
                     /locus_tag="PKI33_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36205.1"
                     /translation="MKIYNRLFLVFVLTALLSACGAFQPAAPADKVTVQLGWFHGVEY
                     SGFYAAIDQGYYKDANIEVTLNPG"
     gene            complement(217..2568)
                     /locus_tag="PKI33_04045"
     CDS             complement(217..2568)
                     /locus_tag="PKI33_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36206.1"
                     /translation="MAMNTPVASPKEMVRILVVDDHPNTATTLARALAQLGPTVDVVS
                     AVSGTDALEKVKDKGVDILFTDMIMPEMTGLELIEKMQNHPGGKPAYTYLVTAYDVPG
                     LKVTAQRLKVNDVIIKPVRPEKICQIATAAMDEMKHAANPAGKKSATKRKFKILVADD
                     VMDNVTLLTRYLEYEGYDQVVARDGLEAINKIRDEMPDLILLDVNMPNKDGFTVLSEV
                     RADSAIAHIPVIILTAARLDPSDVQSGLNLGADDYVTKPFDRHELMARIRTKLRVKEA
                     EDITRRRNRELNLLPEIGKQLSARLDPKDLANVLLKRTAETLGAIQANMYILDSEEKE
                     IQEKYEVVLNPSGAAENLTPPAKLIQHVQENHQGVVIEDAVNDPLWQVDAQTHIRSAL
                     VVPIFGRYALLGLIILTHEQESYFSSDHLLLLQAIASQAAIAIENTRLYSVVLQEQKK
                     VAAILRHASEAILLFDEQGNLTLLNPAGEQLFLMHPMKMNQPLPTDYGYDGLIKLIED
                     ARTSQMSISGEIVWVDQRTFTAFVAPIEDGGTVAILHDVSRFKDLDQVKNEFIATASH
                     DLKNPLTSIGGFAQLLLQAGPLNENQKEFVDRIQHSVKSMSELIQNMMALAQADLNAT
                     QKHEPVDIKEVLDEVVNEFAPQARGKEQNLEFTSPTSHVQVNGDSLHLRQAFRNLLGN
                     AIKYTPKGGLIQVQSVIEQGNLLVHFKDNGYGIPATDLPFIFNRFYRVRTGKASEVEG
                     NGLGLAIVKAITVEHNGSVNVESQAGKGSCFSITLPLLNNNFA"
     gene            complement(2713..>4281)
                     /locus_tag="PKI33_04050"
     CDS             complement(2713..>4281)
                     /locus_tag="PKI33_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNM36207.1"
                     /translation="DECTWGNCGVRVMGNAHFARGDTNVFSMCPAGGEHGTRGYTNWN
                     AWVISAIKTIIHIEGGIPTFTGYTAPTLTVTNACVTSTLPGLSVSGTQLMANGKVIRL
                     RGVNMGDPFWARGWYGSMYSTSNYSTLAQDWHANVVRISIFPTQWKNMDRATLLAGLD
                     TEIQAALSNGMYVIISYHVIGWPDGWYKEAYPGNPTDTYDSSMSVATSFWTKMAQTYG
                     GDTRIMFDLWNEPVHPDDFTLYGSTPNPLWAPLKSMYESLIQAVRNNGGQNIVIATGN
                     RWASWLVGIKDNPLADSKVIYAYHKYSVTGYNTASVWNNDLGGLLGVKPIIVTEWGYE
                     DSDVTNPSWPGTQASYGDPFTQWLDSNNLSNLAWMYHHDWTPALIKSDSTTTLYGTFV
                     KGYFTNLSIGTTKPDLIVTNVTVNPTMPGNNQTFTVNITVKNQGASTGANTIYRDVYI
                     DRDPSTLLDPITGCPPPGDFYTSDAYASIPAGMTDTKSVTVTGGLPTGNHQIWVYVDA
                     RCLIDEGANSNNTP"
BASE COUNT         1094 a          991 c         1033 g         1163 t
ORIGIN      
        1 cctggattaa gcgtgacttc aatattggca tctttgtaat atccctgatc aatggcagca
       61 taaaagccgg aatattccac accatggaac cagcctaatt gaacggttac cttgtctgcc
      121 ggagccgctg gttgaaatgc cccacatgcg cttaatagtg cagtaagaac aaaaactaaa
      181 aacaaacgat tatagatttt cattgggttc ctctttctag gcaaaattat tattcaacaa
      241 cggcaaggtg atgctaaagc aagacccctt cccggcttga ctttctacgt ttactgaacc
      301 attatgttca actgtgatgg cttttacgat ggcaagccct aacccattgc cttccacttc
      361 gctggctttc ccagtgcgaa cgcgataaaa acgattgaaa ataaaaggca ggtcagtagc
      421 agggatgcca tatccattgt ctttgaaatg aacgagcagg ttcccttgtt cgattacaga
      481 ctggacctga ataagcccgc ctttaggcgt gtacttaatg gcgtttccca gtaaattccg
      541 gaatgcttgg cgtaaatgga gcgagtcacc atttacctgc acatgagagg tgggtgaggt
      601 gaattcaagg ttttgttctt tgccgcgcgc ttgtggagcg aactcattaa ccacttcatc
      661 caaaacttct ttaatgtcaa caggttcgtg cttttgggta gcgttcaaat ctgcctgagc
      721 caatgccatc atattttgga ttaattcgct catgctctta acggagtgtt gaatgcggtc
      781 tacaaactct ttttggtttt cgttaagtgg acccgcctga agcagcaatt gcgcaaaccc
      841 accaatcgaa gtaagcggat tcttcaagtc atgtgaggcg gtggcgatga attcattctt
      901 gacctgatcc aagtctttga agcggctcac atcgtgcaag atggcaacgg tcccgccatc
      961 ttcaattgga gcgacaaagg cagtgaatgt gcgttggtca acccaaacga tttcgcccga
     1021 aatagacatt tgagatgtac gtgcatcttc aattaactta ataagcccgt cgtatccata
     1081 atccgtaggc aaaggctgat tcattttcat ggggtgcatc agaaaaagct gttcacctgc
     1141 agggtttagc aaggttaggt tgccttgctc atcgaagagc aaaatcgctt ctgaggcgtg
     1201 tcttaagatc gcagcaactt ttttctgctc ttgcaatacc acggaataca atctcgtgtt
     1261 ttcaatggca atggcagcct ggcttgcaat ggcttgtaat aacaacaaat ggtcgcttga
     1321 aaaataactt tcctgttcgt gagtcagaat gatcaacccc aataatgcat aacgaccaaa
     1381 gatgggcaca accaaagccg aacggatgtg ggtttgagca tccacttgcc agagcggatc
     1441 attgaccgca tcttcgatta ccaccccttg atgattttcc tgtacgtgtt gaataagttt
     1501 ggcaggcgga gtaaggtttt cagcagcccc tgaaggattc aaaaccactt catacttttc
     1561 ttgaatctct ttttcttcag agtctaaaat atacatattg gcttgaatag cgcccaaagt
     1621 ttcggcggtg cgcttgagca aaacatttgc aaggtctttg gggtctaaac gagcgctgag
     1681 ttgtttgcca atttcaggca ataaatttaa ctcgcggttg cggcgacgcg taatatcttc
     1741 ggcttctttc acacgtaact tggtgcgaat gcgcgccatt agctcatgac ggtcgaaagg
     1801 cttggtaacg taatcatcgg caccaaggtt caagccagat tgcacatcag agggatctaa
     1861 acgagcggcg gtcaaaataa tgaccggaat atgagcaatt gccgaatctg cgcggacttc
     1921 tgaaaggaca gtgaagccgt ctttgttcgg catattaaca tccaatagga tgaggtctgg
     1981 catttcatca cgtatcttgt ttatcgcttc cagcccgtca cgcgcaacaa cctggtcata
     2041 cccctcatat tcaagataac gggttaacag agtgacgtta tccataacat catctgctac
     2101 gagaatttta aatttgcgtt tggtcgccga tttcttcccc gcagggtttg cggcatgttt
     2161 catttcatcc atcgcagcgg tagcgatctg gcaaattttc tctgggcgca caggcttaat
     2221 gatgacgtca ttaaccttca agcgttgcgc cgtaacctta agacccggca catcatacgc
     2281 agtgaccaga taggtatacg caggcttgcc accggggtga ttttgcattt tttcgatcaa
     2341 ttccagcccg gtcatttcag gcatgatcat gtctgtaaag agaatatcca cacctttatc
     2401 tttgaccttt tcaagcgcat cggtaccgct cacagccgaa accacatcta cggtgggtcc
     2461 aagctgggcg agcgcacgcg caagagttgt tgctgtattt gggtggtcat ccacaaccag
     2521 aatgcgcacc atttctttgg gagaagcgac tggggtattc atcgccatat attctcgccg
     2581 gattctccgc aagggtagtt aggttggata ggataaaagt tgggaaaagt tgggaacgaa
     2641 aaacccgcct caggcgtgga ccggaggcgg gtttttgtcc attaggcgat tacctcgctg
     2701 taaaaatccg aactatggag tattgttgct gtttgctccc tcatcgatca ggcaacgcgc
     2761 atctacatat acccaaattt ggtggttgcc agttggaagc ccgccggtga ctgtgactga
     2821 cttggtgtcg gtcatgccag cgggaatact ggcataagca tcggaggtgt agaaatcgcc
     2881 gggcggaggg cagccggtga tggggtcaag cagggtagaa gggtcgcggt cgatgtatac
     2941 atcccggtag attgtgttgg ctccggtgga agcgccttga ttctttacag taatatttac
     3001 ggtaaaggtc tgattatttc ccggcatggt tgggttgacg gtgacattgg tgacgatgag
     3061 atcaggtttg gtagtgccaa tggaaaggtt ggtgaaataa ccttttacaa acgttccata
     3121 aagcgtagtt gtgctatctg atttgattag tgcgggcgtc cagtcgtgat gatacatcca
     3181 tgctagatta ctgagattgt tactgtccag ccattgcgtg aatggatcac catacgaggc
     3241 ttgcgtgccg ggccaggatg ggttggtcac gtcactgtct tcgtagcccc actcggtgac
     3301 gatgatcggc tttacgccaa gtaacccgcc taagtcatta ttccaaacgc tggcagtgtt
     3361 atagcctgta actgaatatt tatgataggc gtagatcact ttggagtcag cgaggggatt
     3421 atctttgata cccactaacc aacttgccca acgattgccc gtggcaatga caatgttctg
     3481 tccgccatta tttcttaccg cttgaataag actttcatac atgctcttca agggcgccca
     3541 taacggattt ggtgtggaac catacagcgt aaaatcatct gggtggacag gttcattcca
     3601 taagtcaaac atgatgcgag tatcgccgcc atatgtctgt gccatttttg tccagaatga
     3661 agttgccaca ctcatgctgg agtcgtaagt atctgtaggg ttgcctggat aggcttcttt
     3721 gtaccagcca tcgggccaac caatgacatg gtaagaaatg atgacataca tgccgttgct
     3781 aagtgcagct tgaatttctg tatctaaccc tgccaataaa gtggcacgat ccatgttttt
     3841 ccattgagta gggaagatac taatacgaac aacgttggca tgccaatctt gagcaagcgt
     3901 cgaataattt gacgtcgaat acatagaccc ataccagccg cgcgcccaga atgggtcacc
     3961 catgttgacg ccgcgtaaac ggatgacttt tccatttgcc ataagttgtg tgccagaaac
     4021 tgaaagcccc ggtagagtgg atgttacaca agcattcgta actgtcaaag tcggtgcagt
     4081 gtatccggtg aacgtgggaa ttccaccttc aatatggata atggtcttga tggcggagat
     4141 cacccatgcg ttccaattgg tataaccgcg tgtgccatgt tcccctccag ctgggcacat
     4201 gctgaatacg ttcgtgtcac ctcgtgcaaa atgtgcgtta cccattacac gcacgccaca
     4261 attaccccat gtacattcat c
//
LOCUS       DAOREF010000140         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12283_length_4256_cov_3.786955, whole genome shotgun sequence.
ACCESSION   DAOREF010000140 DAOREF010000000
VERSION     DAOREF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12283_length_4256_cov_3.786955"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..681
                     /locus_tag="PKI33_04055"
     CDS             46..681
                     /locus_tag="PKI33_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36208.1"
                     /translation="MKHDSFKSLIAVLTAIVTVLGATAACLASVAVSTAGDNDFAGLD
                     ASIRGQKAEIINYVVAYEHYRAFTSYVRYNELGNLMYDPGADQDTDARNGAFQREVWG
                     IASGISSVFFSPRYITSEGQYDLERELQEEYAKDAQSEDLNPAPYFEESDRQRNISAT
                     LTADMIVFAVSFWFLTLAQATEKKIKYVWAVLGGLMGLAGIIGIFIGRFLQ"
     gene            678..2369
                     /locus_tag="PKI33_04060"
     CDS             678..2369
                     /locus_tag="PKI33_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM36209.1"
                     /translation="MKTENTSEQRFENMVTIMIASVAIWVAITAYFQNYASNISDQAR
                     RRAQQYAIEATKTEVNGTIQFSYDWQGAFQTWRELSWQITAAQQNGDTKAVERYQELQ
                     ARIAPLSKMLAPQYFDPTVGWPESYKYEAESYLVESTRLSETYLAESDLGNFTDNVAD
                     SLIVQITLLTVSLSLYGLSMALKGRVRWLFIIVGSGIVGVCVLWLGWSLLELLIRPEV
                     NQAAITAYSEGVGLAYQGRYQESIDKFTQAINENQYYAKAYYERGLSYYELGDLQTAI
                     SEMEEARTQGMQEDVSLNWNLGWTYYLAGQYDKAIEINEPILSDNPEVLGMRMNQAIS
                     YLSKGDFENSQKQYDLLLQEAQRQVNEARSQGAEPSASLWFYMDAGALDLQNLIDQLD
                     NNPKDWTQAPEANLITGDHSAIRDFATSQMKRLKESTVALEYTGQLPNTQSEIQVEPF
                     VFGQITGTDAQGFITGFEPASNNIIPYGEDSFTLEFTYSGTPPKQMLWKVYVNGYEDQ
                     SLRIVSTDDISDGATWYKTFGYSYTNIFILSPGEYTVELYADNILVQRGTFYVQE"
     gene            2424..3164
                     /locus_tag="PKI33_04065"
     CDS             2424..3164
                     /locus_tag="PKI33_04065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNM36210.1"
                     /translation="MPTPIRYLIFDLGGTLMHSRADWGPIHEQADEALSQSLRENGIE
                     LSAEVFRSRLHDYYQQRDKDFYETTYHLVLRELLKELGHAEVAESVLRSALDALYVIT
                     QSNWELESDAMETLENLKSQNYKLGIFSNAGDDKDVQELVESFGIRSYFDFVLTSAAC
                     FYRKPHPRAFEIALAQWNATPAEAAMIGDSLEADIIGANELNITSIWLTRRAQFKTED
                     MHRIKPDFSLRKLSELPPTLDLLSFSRR"
     gene            3211..3612
                     /locus_tag="PKI33_04070"
     CDS             3211..3612
                     /locus_tag="PKI33_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM36211.1"
                     /translation="MLNFKAETQNARVPVSVLRITGDIDSSNFQTFQTQVDDLVKNGA
                     RHILLDFSNVKRISSAGLRVIHNLFNKLRDIHKDVNDDELRKQMSSGAYKSPYLKLFN
                     LAPQIAEIFSLSGFDIYIQTFDDETEAVQSF"
     gene            3642..>4256
                     /locus_tag="PKI33_04075"
     CDS             3642..>4256
                     /locus_tag="PKI33_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM36212.1"
                     /translation="MPDLFDHAMQERMKSEAPLAARMRPRTLEEYIGQEHIIGEGKLL
                     RRAIEADRLFSSIILWGPPGTGKTTLAQVIANTTRSHFVTISAILAGKADLRVVIDEA
                     LERRRLHNERTILFVDEVHRWNKAQQDALLPHVENGTFTLIGATTENPYFEVIKALIS
                     RSRVFQLRNLNQAETGTLLDRALADKERGYGNKNIQFDEDARNHL"
BASE COUNT         1193 a          951 c          952 g         1160 t
ORIGIN      
        1 aagggtgccg cataatcgtc ttatcagcca aaggagagat gagttatgaa acatgatagc
       61 tttaaatcat tgatcgctgt tcttaccgcc atcgtaaccg tgctaggagc gacagcggct
      121 tgtctggcat cggttgcagt atctactgct ggtgataatg actttgccgg cttggatgcg
      181 tcgattcgtg gtcagaaggc agagatcatc aactatgttg tcgcgtatga gcattatcgt
      241 gcttttacca gttatgtgcg ctacaacgag ttgggtaact tgatgtatga ccctggagca
      301 gaccaagata cagatgcgcg taatggcgct tttcaacgtg aagtttgggg tattgccagt
      361 ggtattagtt ctgttttctt tagcccgcgt tatattactt cagaaggtca gtatgacctt
      421 gaacgcgagt tacaagaaga atacgcaaaa gatgctcaaa gcgaagattt gaatcccgct
      481 ccctatttcg aagagtctga caggcaacga aatatctctg caactctcac tgccgatatg
      541 atcgtgtttg ctgtttcatt ttggtttcta actcttgccc aagccaccga aaagaaaatt
      601 aagtatgttt gggctgtgct gggtggtctg atgggcttgg caggtattat tggaattttt
      661 attgggaggt tcttacaatg aaaaccgaaa atacctctga acaacgcttt gaaaatatgg
      721 tcaccattat gatcgcatcg gttgcgatct gggtggcgat cactgcttat ttccaaaatt
      781 acgcctccaa tatttcagat caagcacgcc gccgtgccca acaatatgcc attgaagcta
      841 cgaagaccga ggtaaacggg accattcaat ttagctatga ttggcagggc gcttttcaaa
      901 cctggcgcga actaagctgg cagatcacag ctgcgcaaca aaatggagat acaaaggcgg
      961 tggaacgcta tcaagagttg caagcccgta ttgccccgct tagcaaaatg cttgcgcctc
     1021 aatactttga cccgactgtt gggtggcccg aatcatacaa atatgaagct gaatcctatt
     1081 tggtcgagtc gacacgcctc tcagaaacgt atttggcgga gtctgattta gggaacttta
     1141 cagacaacgt tgccgattca ttgatcgtgc aaattacttt gcttacggtt tctctatcgc
     1201 tttatggtct ttccatggca ttaaagggac gcgtccgctg gttgtttatt atcgtaggct
     1261 caggcattgt tggggtttgt gttctctggc tgggatggtc tttacttgag ttgttaattc
     1321 gccctgaagt caaccaagcg gctattacag catactctga aggtgttggg ctcgcatatc
     1381 aaggacgata ccaagaatca attgataaat tcacgcaagc tatcaacgaa aatcagtatt
     1441 atgcaaaagc ctattacgaa cgcggacttt cttattatga attaggagac ctgcaaactg
     1501 ccatttctga aatggaagag gctcgcactc aaggtatgca agaagatgtg agcctgaatt
     1561 ggaatctagg ttggacgtat tacctcgccg ggcagtacga taaagcgatt gaaattaacg
     1621 agcccatctt aagtgataac cctgaagtgc ttggaatgcg gatgaatcaa gccatcagtt
     1681 atctttccaa aggggatttc gagaattcgc aaaaacaata tgaccttctg cttcaagaag
     1741 cacaaaggca agttaatgag gcacgctcac aaggtgcaga accttccgct tccttatggt
     1801 tttacatgga tgccggcgct ttggatcttc aaaacctaat tgaccagtta gataacaatc
     1861 ccaaagattg gacccaagcc ccagaagcaa atttgatcac gggcgatcat tctgccattc
     1921 gagactttgc tacttcacaa atgaaacgtt tgaaagaatc tactgtagca ttggaatata
     1981 cgggtcaact gccgaatact caaagtgaaa ttcaagttga gccgtttgtc tttgggcaaa
     2041 tcaccggaac cgatgcgcaa ggttttatca ctggcttcga gccggcaagt aataacatca
     2101 ttccgtatgg cgaagactct ttcacactcg agttcaccta ttcaggaact cctcctaaac
     2161 aaatgctgtg gaaggtatat gtcaacgggt atgaagacca atccttgagg atcgtctcta
     2221 ccgatgatat tagcgatgga gccacttggt acaaaacttt cgggtatagt tatacaaata
     2281 ttttcatttt gagtccgggt gaatacaccg tggaacttta tgcagataac attcttgtac
     2341 aacgaggaac cttctacgtg caagaatagt tatcatttat aagacaggga gtcgttttct
     2401 cgaacggctc cccactaaaa aacatgccta cacctattcg atatttgatc tttgacctcg
     2461 gcggaaccct aatgcactcg cgtgcagatt ggggtcctat tcacgaacag gcagatgaag
     2521 cgctgtcaca atctttgcgc gaaaacggta tagaactcag cgccgaggtt ttccgctcca
     2581 gattacacga ttattaccaa caacgcgata aagattttta cgaaacaacc tatcatcttg
     2641 tcttgcgaga attgctcaaa gagttgggtc atgctgaggt cgcagaatcc gttttacggt
     2701 ccgcgctgga tgcgttgtac gttatcaccc aaagcaattg ggaattagag tctgatgcca
     2761 tggagacact cgaaaacctc aagtcgcaaa attataagct aggcatcttt tccaatgctg
     2821 gcgatgacaa agatgttcaa gaacttgtag agtcctttgg tattcgcagt tattttgatt
     2881 ttgtgcttac ctctgcggca tgtttttatc gcaaaccaca tccgcgtgcg tttgagatcg
     2941 ctcttgcgca atggaacgcc accccagccg aagcggcaat gatcggtgat agtctcgaag
     3001 cggatattat tggcgcaaat gaattgaaca taacttccat ttggttgaca cgccgtgcac
     3061 aattcaaaac tgaggatatg catcgcatca aacccgactt tagcctgcgc aaactctcag
     3121 agctgccacc cacgcttgac ctactctctt tctcccgccg ttagaccaaa gcacttcatc
     3181 taaaatcaaa actcatcaaa ggaaatgtta atgttgaatt tcaaagctga aacacaaaat
     3241 gcgcgcgtac cagtgagtgt cctgagaatc acaggggata ttgactcgtc gaatttccaa
     3301 acgtttcaaa cacaagtaga cgatctggtt aagaatggcg ctcggcatat tctcctagac
     3361 tttagcaacg tcaagcgcat tagcagtgct gggctgcgcg ttattcacaa tctgttcaat
     3421 aaattgcgtg atattcataa agatgtaaac gatgatgaat tgcgaaaaca aatgagttct
     3481 ggggcatata aatcacctta tttgaaactt ttcaacttgg caccgcaaat tgcagaaatt
     3541 ttctcactta gtgggtttga tatttatatt caaacattcg atgatgagac agaagccgtt
     3601 caatctttct agacaattta cttcacgcta taatcctctc catgccagat ctctttgacc
     3661 acgccatgca agaacggatg aaatctgaag cgccacttgc cgcgcgcatg cgtccacgca
     3721 cattagaaga atatattggt caggagcata ttattggtga agggaaactt ttacgtcgcg
     3781 ccattgaagc ggatcgtttg ttttcctcta tcatcttatg gggacctccc ggcacaggca
     3841 aaactacact tgcacaagtg attgcaaata ccacacgcag tcactttgtg accatctctg
     3901 ctattttggc aggcaaagcg gacttgcgtg tggtgatcga tgaagcactg gaacgtcgcc
     3961 gtttgcacaa tgaacgcacc attcttttcg ttgatgaagt tcatcgctgg aataaagccc
     4021 aacaagatgc ccttctccct catgtggaaa atggcacttt cacacttatt ggtgcaacga
     4081 ctgaaaaccc ctactttgaa gttattaaag cgttaatttc gcgttcgcgt gtctttcaac
     4141 tacgcaacct aaaccaagct gaaactggca ctctgttaga ccgcgcgctg gcagacaaag
     4201 aacgtggtta cgggaacaaa aacatccagt ttgatgaaga tgctcgaaat catctt
//
LOCUS       DAOREF010000141         4243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12350_length_4243_cov_4.068529, whole genome shotgun sequence.
ACCESSION   DAOREF010000141 DAOREF010000000
VERSION     DAOREF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12350_length_4243_cov_4.068529"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKI33_04080"
     CDS             <1..389
                     /locus_tag="PKI33_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36213.1"
                     /translation="SIGGEQWVDRFYKICSHPDVDEFYQLEQVGLPKKNGDPYERNNR
                     WALYSSLVRGISKTTLIAVWNNVGGRAKDRDEYLTQHMVDLMRETGGKIEAINTTKHL
                     FEGTTVATTFTQASAPKKTKSAKTKK"
     gene            complement(413..1063)
                     /locus_tag="PKI33_04085"
     CDS             complement(413..1063)
                     /locus_tag="PKI33_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36214.1"
                     /translation="MYQGYGFFEQIDVETCFGIISSAKRRQFRLDASIKVGVSFTAKS
                     DMMSFRRLTPKNYGEQMTEPILLTSESPILSELNFKPYRSSVVRKVKPFLPAQNEPQS
                     IDVQTPWGATLTAKHGDMLLSELDAPNDVWPVNGEIFSETYMIIERGYCVKSAVTMLV
                     PMTDVTNGDEERHVTIETMEGPQTVRAGDFYLARGVKGEIWSIPKEKVASIMKPVE"
     gene            1127..3598
                     /locus_tag="PKI33_04090"
     CDS             1127..3598
                     /locus_tag="PKI33_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36215.1"
                     /translation="MTDANENQTDKKLEASGNPIPVPRETKAAVSTDNNQVMDSSATS
                     NPALPAIKAPGDSSSKAESVPSGTEPKAPKQPRVSDDRDYDIDGSNKDKSLHPEVKPH
                     VFVVMPFGNKKGADGHPFDFNAIYKTLIRPAIEDAGFEPFRADEETASGDILTDMFQE
                     LLLADMVICDLSIDNANAFYELGIRHALRKRGVVHIQAGRAYMPFDVFNVRTLPYHIT
                     SEGLPDPAYIRSDIKAIARLLKDTWASDRELVHSPIYNLLTGLKEPSRKSLQTPLATG
                     FWREYEEWKDRVTIAQRNKHIGDILLLTEEIRNPLIREDAVAEVGGALARLGRNELAL
                     KQYREGIDVNGENLEFRREEAFHLNRVGRVNEAIVKLESILNDYPEDNKSISYLGRIY
                     KEMWTNSWIKVKDKNKRLRLAFESYHWLIQSIKIYMKGFQVDLRDYYPGINAYTLSKI
                     AIHLADKFDNKKTPDPDITRIRHRLHGLQETLEFCLENRLDLDQEKADYWSLVSLAEL
                     RLFTSNDIAEVLRSYRKAVTAVRRDLFSLRSSLEQLETIHSLGIRTEFVSACIKMFKE
                     EIKNASTGDAGNVIKVDNLVRPGTKNKGARALVFSGYMVDFAGKDKKTFPPEKESEIR
                     QEIKKRVDKFKPGPNDRAFLAGLSAGSEIIFAEICAEAGIKVKVFLPHSDSSYIRRFV
                     TPAGESWVDRFYKIRNHSLVDEIYQIEHLGQPKDDDNVYERNSRWAMYSAMGRVGIDN
                     MTLIAVASEFIGDTKDRDIQITRYTIELMRNLGGRVEEFINPTKYIYSVIDSALERLI
                     AQGGSSGEKKITPTKLRKRKSERKV"
     gene            3647..>4243
                     /locus_tag="PKI33_04095"
     CDS             3647..>4243
                     /locus_tag="PKI33_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNM36216.1"
                     /translation="MNKLFVIVGASGVGKTTLMNALLTKYDCAIAYEQHDERPFQKLF
                     KEDARYALANQVDYLLMRAEQERELRSGNKPALMDGGFDLDFYGFTRLFHTHGWLSAA
                     EFDLCRRLHTLTRQLLPAPDLIIHLHADAETVRQRLASRNRINIASSEDAELLESYVN
                     EWLETVPGTNILRLDVSNENASYPVSVPRILDKIRGTHG"
BASE COUNT         1245 a         1002 c          924 g         1072 t
ORIGIN      
        1 tttcaattgg cggcgaacaa tgggtagacc gtttctataa aatatgcagc catcctgatg
       61 tggatgagtt ttatcaacta gaacaggtcg gcttacccaa gaaaaatggc gacccttatg
      121 aacgtaacaa ccgctgggcg ctttattcct ccctcgtacg cggcatttcc aaaaccactc
      181 tcatcgccgt ttggaataat gttggtggac gcgcaaaaga ccgtgatgag tatctgacgc
      241 aacacatggt agatctgatg cgcgaaaccg gcggtaagat tgaagcgatc aataccacca
      301 aacatctttt tgagggcact acagttgcca caactttcac acaagcatca gccccgaaaa
      361 aaactaaatc agccaagacc aagaagtaaa ggcaaaaatg aaacccgtcg agttactcga
      421 cgggtttcat tatggaagct actttttcct ttgggatgga ccaaatttcg cccttcaccc
      481 cgcgtgccag ataaaaatca cccgcccgaa ctgtttgtgg accttccatt gtttcaatgg
      541 tcacatgccg ttcttcatca ccatttgtaa cgtctgtcat tggaacaagc atggttacag
      601 cgctcttcac acaatagcca cgttcaataa tcatgtacgt ttcagagaaa atctcgccat
      661 ttactggcca tacatcgttt ggagcatcca attcactcaa aagcatatcg ccgtgtttag
      721 cagttaaggt ggctccccaa ggggtttgga catctatcga ctgcggttca ttttgagctg
      781 gtagaaaggg ttttactttt cgtacaacag agctacgata gggtttaaaa tttagctcac
      841 ttaaaattgg gctttcagaa gtcaataata tgggttccgt catttgctct ccatagtttt
      901 tcggtgtcaa ccgtcggaaa gacatcatat cgctttttgc tgtaaaactc actcctactt
      961 taatactagc atctaaacga aattgccttc tctttgcaga agagattata ccaaagcagg
     1021 tttccacatc tatctgctca aaaaagccat atccttgata caatttaggg gtattgataa
     1081 aaccgcaaat cttggaacct gactttacct actagaggaa cctcacatga cggatgcaaa
     1141 cgaaaaccaa acagataaaa agttagaggc aagtggaaat cccatccctg tccctcgtga
     1201 aaccaaggct gccgtttcta cagacaacaa ccaagtcatg gattcttctg caacttcaaa
     1261 tccagccttg ccggcgataa aagcgcccgg tgattcttcg tctaaggcag aatcggttcc
     1321 atcagggaca gagccaaaag cgccgaaaca accccgagtt tcggatgatc gggattatga
     1381 catagatggt tccaacaaag acaagtcgct gcatcctgaa gtcaaaccgc atgtttttgt
     1441 tgttatgcca tttggtaaca aaaagggagc cgatggtcat ccgttcgatt tcaatgcaat
     1501 ttacaagacc ttaatcagac ctgccatcga agatgccggc ttcgagcctt ttcgagcaga
     1561 tgaagaaacc gcctcaggcg acatcctgac cgacatgttc caagagttgc ttttggcaga
     1621 tatggtcatt tgcgatctaa gcatcgacaa cgccaacgct ttttatgaat taggaattcg
     1681 tcatgcgtta cgcaaacgtg gtgtggttca tattcaagcc gggcgcgctt acatgccatt
     1741 tgatgtgttc aacgtacgta ctttgcccta tcacatcaca tcagaaggtc tgccagatcc
     1801 ggcatatatt cgcagtgaca ttaaggcaat tgcgcgcctt ctcaaagata cctgggcttc
     1861 agaccgagaa ctagtacata gcccaatcta caacctgctc acagggctta aggaaccaag
     1921 ccgcaaaagt ttgcaaaccc ccttagcaac tggcttttgg cgcgaatatg aggaatggaa
     1981 agacagagtc accattgctc aacgcaataa acacattggt gatattttgc tcttaactga
     2041 agaaatcaga aacccgctca ttcgcgaaga tgcagttgct gaagtaggcg gcgcacttgc
     2101 ccggttggga cgtaacgagt tagctctaaa acaataccgc gaagggattg acgtcaacgg
     2161 ggaaaatctt gaatttcgcc gggaagaagc ttttcacctt aaccgtgtag gaagggtgaa
     2221 tgaagccatc gtaaaacttg aaagtatttt gaatgattat ccagaagaca acaaatcaat
     2281 ttcatatctc ggacgcatct acaaagagat gtggaccaac tcttggatca aagttaaaga
     2341 caagaacaag cgccttcgct tggcatttga aagctatcac tggctcatac aatccatcaa
     2401 gatttatatg aagggatttc aagttgacct gcgggattac taccctggga tcaatgcata
     2461 caccctttcc aaaattgcca ttcacctcgc agataagttt gacaacaaaa agacgcccga
     2521 cccagacatc actcgcattc gccacagact gcatgggtta caggaaacgc tagaattttg
     2581 tctggaaaat cggctcgatc ttgatcaaga aaaagctgat tactggtctc ttgtatcttt
     2641 ggcagaatta aggctcttca cttccaatga cattgctgaa gttttgcgct cctatcgcaa
     2701 ggcagtcaca gccgttcgac gcgatctatt ttctctgcgt tcatcccttg aacaactgga
     2761 aacgattcat tctctcggca ttcgcactga atttgtttca gcttgtatca aaatgttcaa
     2821 ggaagaaatc aaaaatgcca gcacaggcga tgcaggcaat gttatcaagg tagataactt
     2881 ggtacgccca ggcacgaaaa ataaaggggc tcgagcactc gtatttagtg gctacatggt
     2941 cgactttgcg ggaaaagata aaaagacatt cccgcccgaa aaagaaagtg aaatccggca
     3001 ggaaatcaag aaacgtgttg acaagttcaa acccggaccc aatgaccgcg catttttagc
     3061 aggtttatct gcgggtagtg aaatcatctt cgcagagatt tgtgccgaag ccggcataaa
     3121 ggtcaaggtg ttcttacccc actctgattc cagttatatt cgccgtttcg taacacccgc
     3181 tggcgaaagt tgggtggatc gcttctacaa aattcgcaac cactcattag tagatgagat
     3241 ctaccaaatc gagcacctcg gtcagcccaa agatgacgat aatgtctatg agcgaaacag
     3301 ccgttgggca atgtattcag ccatggggcg cgttggcatt gataacatga cactaatagc
     3361 tgtagcaagc gaattcattg gcgacaccaa agaccgcgat attcaaatca ctcgttacac
     3421 gattgaattg atgcgcaatc tgggtggcag ggttgaagaa tttatcaacc ccaccaagta
     3481 tatttacagc gtaatcgata gcgctcttga acgcctcatt gcacaaggtg gttcttcagg
     3541 cgaaaagaaa attacaccta ccaaactgag gaaaagaaaa tctgaaagaa aggtttagca
     3601 aaaccataga cccgcttttg cagcgggtct attcatccta aacgctatga ataaactttt
     3661 tgtcattgta ggtgccagcg gagtaggcaa aaccacgctt atgaatgcct tgcttacaaa
     3721 gtatgattgc gctattgcat atgagcaaca tgacgagcgt ccattccaaa aacttttcaa
     3781 agaagatgca cgctatgcgc tggcgaatca agttgattat ctattgatgc gcgccgaaca
     3841 agaacgtgaa ttacgctctg gcaataaacc cgctttgatg gatggcggct ttgaccttga
     3901 tttttatgga tttacacgcc tctttcatac tcatggctgg ctcagcgctg cagaatttga
     3961 cctgtgtcga cggctgcata cacttacccg tcaactgtta cctgctcctg acctcatcat
     4021 ccatctccac gcggatgcag aaactgtccg acagaggctg gcttcgagaa accgaatcaa
     4081 catcgcgtct agtgaagatg cggagctttt agagtcctat gtcaacgaat ggctggagac
     4141 ggttccaggg acaaatattc tgcggctgga cgtttccaac gaaaatgcga gttatcctgt
     4201 cagcgtaccg cgcatccttg ataaaattag gggtactcat gga
//
LOCUS       DAOREF010000142         4239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12368_length_4239_cov_3.496176, whole genome shotgun sequence.
ACCESSION   DAOREF010000142 DAOREF010000000
VERSION     DAOREF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12368_length_4239_cov_3.496176"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="PKI33_04100"
     CDS             complement(<1..1725)
                     /locus_tag="PKI33_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36217.1"
                     /translation="MTHQILSIIPLGVLSFSCFITALAIQIHCVWGLIHKRETLIGII
                     TITLLFIVSFFLIQSGQYISAAAMILAGIYQTYIILPIDKKRKKVDFTNLSIATITFA
                     TGIILTVTSSQDDAKLLLATSFLILAFVAGTFIVIPSFKYGQWLNKTQVIPWLILVFF
                     YILPTGEGNLVVPAAVVGTLLFSRFIPWDSLSMPANDILSRRVIVIAFVVEIANLLFL
                     GALLFAVEPEFKNNYSAIFITRGLTLAFFILSSILIYIGVMTVIMTTNGLTQELNRIG
                     FEDEENGELLEDSESPWTSRLEKYIQPYIMTSEGIRGRIQADKINALTRQSAFDKKRN
                     AQLTLLLELSQQLENQLDPPVSAQLAVNTLERALNCSLVLLFIHEPEKKEFMLLAEAG
                     RQTHLVPKEYRQDASVGVLGRALRQRKTQVVNNIQVDPDYIPFKNENNLSAAVIPLIY
                     NGHIHGAIALSNEKMNTLGSLEIGMAETVAGELVRAWERSGYHERLKELIQTGSQLSS
                     VPDPETAVQEVALISKEIVQAKFTYIYIQLGQERNFIQHASAGQAPQLLTSLREASIS
                     AELINLALH"
     gene            1984..2820
                     /locus_tag="PKI33_04105"
     CDS             1984..2820
                     /locus_tag="PKI33_04105"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNM36218.1"
                     /translation="MQKFISLAQIDEIVNVIKKKISIQPVIGMILGSGLNGLADSVQN
                     PVHIPYSDLPNFPTSTVHGHVGRFVIGELEGKPVIVMQGRIHYYEGYTMGEVTLPVRV
                     MHRLGVHSLFVTNAAGGVNENFVPGDVMLITDQLNLMGMSGLNPLMGPNLDEIGPRFP
                     DMSQPYDREYCDLARKVAKQSNLTLREGVYAGLSGPSFESPADLRFLRLAGADAVGMS
                     TVPEVIIARHGGMRVLGLSGVSNKANLDGSTITTHEEVIEAGRVITPKIETIVRGVLR
                     GI"
     gene            2845..3522
                     /locus_tag="PKI33_04110"
     CDS             2845..3522
                     /locus_tag="PKI33_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36219.1"
                     /translation="MVDLFKFLAEYEVLIYIVLAIGALFSFRWLFRSWREWQVAVFSL
                     EREFSARRFGRSALVSAMIVIFFCVEFFMASFIIPGLPADFFVATPTLDLISTPTGTL
                     SPELMTQFANQPQVAQPNAAGCTPGQIELTFPEAGTEIKGIIELTGTVDIPNFGFYKY
                     EIAPSGSDTWATIAAGRTVVKASSLGRWDTTALTPGDYQLRLVVIDNEGNSLTPCVVP
                     VQVIPNQ"
     gene            3539..4078
                     /locus_tag="PKI33_04115"
     CDS             3539..4078
                     /locus_tag="PKI33_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36220.1"
                     /translation="MSELQIRPTVATDLTRLMGIDHSMTSEAVWQLEVRRSTGQVAVT
                     FREVRLPREIQVPYPHNHFALADDWVKRSMMYTAIKDTEPVGYVSLLERGTASTVWVL
                     DLAIDAKQRRRGVAAALLASAQAWAESRSHRRLILEVQSKNVPAIRLAQKSGFEFCGY
                     NDQYYLNKDVALYFAKVLK"
BASE COUNT         1103 a          923 c         1024 g         1189 t
ORIGIN      
        1 atgcaatgct aaattgatta gctcggcaga gatacttgcc tctcgaagcg aggtcagcaa
       61 ttgtggcgct tgccctgctg aggcatgttg aatgaaattt cgttcttgcc caagttgaat
      121 gtagatatac gtaaactttg cctgtacgat ctcttttgag atcaaggcaa cttcttgaac
      181 ggctgtttct gggtctggga cggaagaaag ctgactacca gtttgaataa gctctttgag
      241 acgttcatga tacccagaac gctcccacgc tcgtactaat tcgccagcaa cggtttctgc
      301 catgccgatc tcaagactgc caagcgtgtt cattttttca ttgcttaagg ctattgcccc
      361 gtggatatga ccgttataga taagagggat aactgctgct gataagttat tctcattttt
      421 aaacggaatg taatctgggt ccacttgaat attattaaca acctgtgttt ttctttggcg
      481 taatgctcta cccaatactc ctaccgatgc atcttgccga tattctttgg gaacgagatg
      541 tgtttgtctg cccgcttctg ccaaaagcat gaattccttt ttttcaggct cgtgtataaa
      601 caacaagacc aaactacaat ttaaagctcg ttcaagcgtg ttgaccgcaa gctgtgcaga
      661 tacgggcggg tcgagctgat tttcaagctg ttggcttaat tccaataaca aagttagttg
      721 tgcatttcgt tttttatcaa atgcagattg acgagtaagg gcattgatct tgtccgcttg
      781 aatgcgccca cggatgccct ctgaagtcat aatatatggc tggatgtatt tttctaggcg
      841 actggtccat gggctttctg agtcttcaag gagttcgccg ttttcttcat cttcaaaacc
      901 aattctgttc aactcttgag ttaatccatt ggtggtcatg atcacggtca ttaccccaat
      961 atagatcaaa atcgaggaca agataaaaaa tgccagagtc aagccacgtg tgataaagat
     1021 cgcagaatag ttattcttaa attcaggctc aactgcaaat agtaacgcac ccaaaaataa
     1081 caaattggcg atttctacta cgaaggcgat cacgatcacg cgtcgactta aaatgtcatt
     1141 ggcaggcata ctgaggctat cccaagggat aaaccgagaa aacaataagg tacctaccac
     1201 agctgccggc acgaccaaat tcccttcgcc tgttggaagg atgtaaaaga acacaaggat
     1261 gagccaggga atgacctgcg ttttattcag ccattgccca tatttgaacg acgggataac
     1321 gataaaagtc cctgctacaa aagctaagat caagaacgaa gtcgccaaca aaagctttgc
     1381 atcatcttga gaacttgtga cagttaaaat gatgccagtt gcaaatgtta ttgtcgcaat
     1441 ggataggttt gtgaaatcaa ccttttttcg ctttttatcg atgggtaaaa tgatataggt
     1501 ctgataaatg cccgcaagga tcatggctgc tgctgaaatg tattgtccgc tttggataag
     1561 aaaaaagctg acaataaaaa gcagcgtgat agtaattatg ccaattagag tttcacgttt
     1621 atggatcagc ccccacacac agtgaatttg aattgcaaga gcagtaataa agcaggaaaa
     1681 acttagaacc cccagaggga tgattgagag gatttggtgg gtcatcccaa aatgacttgg
     1741 tatgagagcc attcctgccc caacgccttc tagcagtaag gaaacaaata tctggaaatt
     1801 tttctcgtta gggcgcttca tgtcgctaga ggatagcata agaaacaaat tttatctagt
     1861 tcacttcaat tgtgcatttt agggctgaaa agcccacagg gtcatttctg ttataatcat
     1921 ttcgcaaact ggtctttatg gggctgagcc gtttgtaatc ctgacgatat aaggtaaatg
     1981 taaatgcaga aatttatctc tcttgctcaa atcgacgaga tcgttaatgt cataaaaaag
     2041 aagatttcca ttcagccagt gatcgggatg attctcggct cagggcttaa cgggctggct
     2101 gattctgtcc agaatccggt tcacattcca tacagtgacc taccaaactt ccccacctca
     2161 accgtccacg gacatgttgg tcgctttgtg atcggtgaac tagaaggcaa gcccgttatc
     2221 gtcatgcaag gacgaattca ttattatgaa ggctacacca tgggcgaagt aacgttgcct
     2281 gtgcgtgtca tgcatcgact tggagttcat agtttatttg taaccaatgc ggctggcggt
     2341 gtgaacgaga atttcgtacc aggtgatgtg atgttgatca ctgaccagtt aaacctgatg
     2401 gggatgtctg gtttgaaccc cttgatggga cccaacctcg acgagattgg accgcgcttc
     2461 ccagatatga gccagcctta tgaccgtgaa tattgtgacc ttgctcgtaa agtggcaaag
     2521 caaagtaacc taaccttgcg tgaaggggtc tacgctggtt tgagtggacc ttcctttgaa
     2581 tcaccagcag acctgcggtt cttacgcctt gccggtgcgg atgctgtagg aatgtccaca
     2641 gtaccagaag tgattattgc tcggcatggc ggaatgcgcg tcttagggct ttcaggcgta
     2701 agtaacaaag ctaatttaga tgggtccact attacaactc acgaagaggt gattgaagct
     2761 gggcgtgtaa tcacacccaa gatcgaaacc atcgtacggg gcgttcttcg cggaatataa
     2821 acgcgaactt ggcaaggttg ttagatggta gatctcttta agttcctcgc tgaatacgag
     2881 gtattgattt atattgtctt ggcgattgga gcattatttt cctttcgctg gctttttcgt
     2941 tcatggcgtg aatggcaggt ggctgtcttt agtttggaac gcgagttttc agctcggcgt
     3001 tttgggcgtt ctgctttggt ctcagcgatg atcgtgattt ttttctgcgt agagttcttc
     3061 atggcatcgt tcatcatccc aggtctcccc gctgatttct ttgttgctac accgactctg
     3121 gacctaattt ccacaccaac cggtacgctt tcgcccgaac tgatgaccca atttgcaaat
     3181 cagccgcaag tggcgcaacc caatgctgct gggtgtaccc ctgggcaaat cgagttgaca
     3241 ttccctgagg ctggcactga aattaaaggc atcatcgaac tgacaggtac tgtcgacata
     3301 cccaatttcg gattttataa atatgagatc gctccaagtg gcagtgacac gtgggcgacg
     3361 attgcagcag gacgcaccgt tgtgaaagca agttctcttg ggcgttggga caccacagcg
     3421 ctcacccctg gcgactatca attgagattg gtcgttatcg ataatgaagg taattcgctt
     3481 acgccttgtg tcgtgcctgt gcaggttata ccaaatcaat aaaaggtaat ttcaattcat
     3541 gtctgagtta caaattcgcc caactgttgc tactgatcta acccgtttga tgggaattga
     3601 ccattccatg accagcgaag cagtttggca gttggaagtt cgtcgtagta cggggcaggt
     3661 ggcagttaca tttcgtgagg ttcgtctacc gcgtgaaatt caggtgccat acccgcataa
     3721 tcattttgcc ttggcagatg attgggtaaa gcgctccatg atgtatacag cgatcaagga
     3781 taccgagccg gtcggatatg tcagcttgtt ggagcggggg actgcttcaa ccgtgtgggt
     3841 gctggatctg gcaattgatg cgaagcaacg tcgccgcggc gtggctgcag ctttgttggc
     3901 gtctgctcag gcatgggcgg agtcgcgatc gcatcgtcgc ctgattctcg aagtgcagtc
     3961 aaaaaatgtc ccagcgatcc ggctggcgca gaaatctgga tttgagttct gcgggtataa
     4021 tgaccagtat taccttaaca aggacgtagc actatacttt gcgaaggtat taaagtaagg
     4081 cgagcggttt taccgcccgt tgcacgattt ttattggaag ggttgcatga ttaacggttg
     4141 gccagaaaca ctactggcgg ggcttcaaac gatcaatcag attctgacgg cgggaattgc
     4201 cattacggca ttctcgttgt ttctctattc gctttcatt
//
LOCUS       DAOREF010000143         4228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12419_length_4228_cov_4.522166, whole genome shotgun sequence.
ACCESSION   DAOREF010000143 DAOREF010000000
VERSION     DAOREF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12419_length_4228_cov_4.522166"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKI33_04120"
     CDS             complement(<1..596)
                     /locus_tag="PKI33_04120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36221.1"
                     /translation="MEKPIERPSILQTYTRSFEPLKNANLRMYLGGQAISLLGTWMQS
                     TAQSWVVWELTQSESALGIVVMLNSLPLFLLAPWAGGFADRFNRRAILMVTQATAMLL
                     AFALAFLVQTKLVQIWHIYVFSFVLGTIAALDFPAQQAFIGDLSGMKQVRQAVTLNIM
                     GLQLARMLGPAAAGFLLKAVGSAAAFWINGVSFLVVLF"
     gene            797..1027
                     /locus_tag="PKI33_04125"
     CDS             797..1027
                     /locus_tag="PKI33_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36222.1"
                     /translation="MNDLEKIIIEEVSTLDDMRLIDVIGFIRYLKAEKPIRQEWIAGW
                     YESAIKSMHEREEELRITPTELEKQLRKKQSQ"
     gene            1094..2260
                     /locus_tag="PKI33_04130"
     CDS             1094..2260
                     /locus_tag="PKI33_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM36223.1"
                     /translation="MTTLRTPARIIPRAFPGIKPEEVQEIIVNSQIKSYPAGTILCQE
                     DAIEHTFYMILEGDFEVTKTINNKEKRLLKTLIAGDFFGEMALIHNAPRAATVQSLTP
                     AVVLELDKQGFDRVLKHSPSVATAMVAEISNRLRANDQLAIEDLRLRAREIADAYQKL
                     AEQDMARNEFLTSIAHELRTPLMVASGYLHTLKKGVLTGEQLQSTIEIVSRNVDQIVN
                     LTNDLLFLQEQDLVLRDFQALNLYHVVEGVIEKYQAKAKTRSINIKVRGSANIPSVQG
                     DEPSLERAIAGLVDNAIKFSPYGGTVEIRFADLGNYVSVSVVDYGIGIDPQIRPRIFD
                     RFFHLEKSNDEIYDGLGIGLSITRQVIQQHRGMLDVDSQPGKGSTFTISLPKWK"
     gene            2266..3051
                     /locus_tag="PKI33_04135"
     CDS             2266..3051
                     /locus_tag="PKI33_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36224.1"
                     /translation="MRQTHQLVITILVLVLLTACGGNEQSSNISLPTVLPPAGQTTSD
                     IVSQLCDNPLFPVKQNASWSYFSSGGPNGDFAYTDTITSLTPDGFVLTSQFPALSLTQ
                     NWICAPEGYIAQQLGGGTTASVSMQNMITDFKTIQVSGVSLPRTLTPGMQWKYNLSMQ
                     GAVAMPGEQTQSPGTFALTMQELGTETITVPAGTFDATKIQATFTANIDVDFEGSIIR
                     YTVNGTSLLWYAPGVGFAKSIENIDFSGTSFTSTTELQSYNIP"
     gene            complement(3081..3536)
                     /locus_tag="PKI33_04140"
     CDS             complement(3081..3536)
                     /locus_tag="PKI33_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNM36225.1"
                     /translation="MLIPRTAIFVRRGDEYLLLKGAPTKRLWAGKYNGLGGHVDRGED
                     VLSSARRELMEEAGISADLWLAGTVIVDAGEIGILLFVVVGENPQGEVKGSEEGTPEW
                     VQKEAVSQLPVVEDLPVLLERIHSMKRSSPPFAARSFYENDKLNVVFGE"
     gene            complement(3581..>4228)
                     /locus_tag="PKI33_04145"
     CDS             complement(3581..>4228)
                     /locus_tag="PKI33_04145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNM36226.1"
                     /translation="YMDAYNANLQKLAPGKPGLSKISVLTGTSHGGTVLPDGSIAEVS
                     IAFDVLRDLSRVARKDYGMGGTVQHGASTVPEENFNKFVQNEAIEVHLATNFATMFFD
                     NIPADFKKEIYAWLDVNAAGDRKPGMTDEQFYYKVRKNAIGPFKAKSYALPAESLDKL
                     GKAWEAQFDKLFNLLGLKDTKNQTEKFIKPVKVAPVLADYVKDAVAAEDVSDLSD"
BASE COUNT         1222 a         1058 c          933 g         1015 t
ORIGIN      
        1 ctaaacaaga ccacgaggaa gctcacgcca ttgatccaga aggctgcagc agaaccaacg
       61 gctttcagca aaaagccagc cgcggcggga cccaacatac gcgcaagttg taagcccatg
      121 atgttgagtg tcactgcttg gcgaacctgt ttcataccag agagatcgcc gataaatgct
      181 tgttgagcgg ggaagtctaa cgcggcgatg gtgccaagga caaatgaaaa gacgtaaata
      241 tgccagattt gaacaagctt ggtttgcaca agaaacgcta aggcaaaagc tagaagcatg
      301 gcggtggctt gagtgaccat gagaatcgcg cggcgattga aacggtctgc aaagccaccc
      361 gcccatggcg caagcaagaa gagaggcaaa ctattgagca tgaccacaat gccaagcgcg
      421 ctttcagatt gagtcaactc ccaaaccacc caactttgtg ctgtagattg catccacgta
      481 ccaagcagag aaatcgcttg cccacccaag tacatgcgaa gattggcatt cttaagcggc
      541 tcgaaactac gagtgtaagt ttgaagaatg gaaggacgtt caattggttt ttccaaggaa
      601 ggctcctaag ggattgaaga gattttagac aagaattcgg atgaaatccc aagctgaatt
      661 cttaccaatc actaataaaa caaagtacaa tttgggaaat ccccttaact ataaaccgat
      721 ttcctttcaa aacaatgtcg aggaaatcct atatatttct ccaatgactg tttgtattca
      781 aagtgtagga gctattatga acgacctaga aaaaattatc attgaagagg tttccacctt
      841 ggatgatatg cgtctaatcg atgtgatcgg gtttatccgc tatctcaagg cagaaaagcc
      901 cattcggcag gagtggattg caggctggta tgaaagtgca attaagtcta tgcacgagcg
      961 cgaagaagaa ttaaggatta ctcccacaga acttgaaaaa cagttaagaa aaaagcaaag
     1021 tcaatagtcc taatgggtga ataacagata acctgcccgt gctaaaatca atgtaggatc
     1081 agtatctata agcatgacca cacttcgcac ccctgccaga atcattccgc gtgcttttcc
     1141 cggcatcaaa ccggaggaag tgcaagagat catcgtcaac agccaaatca agagctaccc
     1201 ggctggtacg attctatgtc aggaagatgc gattgaacat actttttata tgatcctcga
     1261 aggggatttt gaagttacaa agaccattaa taataaagaa aaaagattgc ttaagactct
     1321 aattgcagga gacttcttcg gcgagatggc gttaatccat aacgcgccac gtgccgccac
     1381 ggttcaatcg ctaacgccgg ctgtggtgct ggaacttgat aagcaaggct tcgaccgagt
     1441 cttaaaacac agtcccagcg tagccaccgc aatggtggca gagatcagca atcggttaag
     1501 agcgaacgac cagcttgcca tcgaagattt acgcttgcgt gctcgtgaga tcgcggatgc
     1561 atatcaaaaa cttgctgagc aagatatggc gcgtaatgag ttcctaacca gcatcgccca
     1621 cgaactgcga actccgttga tggttgccag tggatattta catactctaa aaaaaggggt
     1681 tctcactggc gagcagttgc agtcgactat tgaaatcgtc agccgcaacg tggatcagat
     1741 cgtgaactta accaatgatt tacttttctt gcaagaacaa gaccttgtgc tacgggattt
     1801 tcaagcactt aacttgtatc atgttgtaga gggtgttatt gaaaagtatc aagccaaagc
     1861 aaagactcgc agcataaata tcaaagtacg tggaagcgcc aatatcccat cggttcaagg
     1921 ggatgagcct tctctagagc gcgcaattgc agggttagtc gataatgcca ttaagttcag
     1981 cccatatggc ggcacagtag aaattcgttt tgcagactta ggcaattatg tcagtgtaag
     2041 cgtggtggat tatggcattg gcattgaccc acagatccgt ccacgtattt ttgaccgttt
     2101 ctttcatctt gaaaaaagta acgacgaaat ttacgatggt ttggggattg gactttcgat
     2161 cactcgtcag gtcattcaac aacaccgcgg catgttagac gtggacagcc agccgggaaa
     2221 aggaagcacg ttcacaatct ctcttccgaa atggaaataa attccatgcg ccagactcat
     2281 caacttgtca tcactatcct agttctggtt ctactcactg catgcggggg caatgagcaa
     2341 tcttcaaata tttcgttacc aaccgtccta ccacccgccg ggcagacgac ttccgatata
     2401 gtaagtcagc tttgtgataa tccacttttt cctgtcaaac aaaatgcatc ctggtcctat
     2461 ttcagcagcg gcggacccaa tggtgatttc gcttacaccg ataccatcac cagtcttacg
     2521 ccagatggct ttgtactcac ctcacaattc ccggctctgt ctctaacgca aaattggatt
     2581 tgtgccccag aaggctatat tgctcaacaa cttggcgggg gcacaacagc cagcgtctcc
     2641 atgcaaaaca tgatcaccga tttcaagaca atccaggtct ctggagtcag cctgccacgc
     2701 acactcaccc ccggtatgca atggaagtac aacctttcca tgcaaggcgc agtcgccatg
     2761 cctggcgaac aaactcaatc cccgggcaca ttcgcgctca ctatgcaaga gctcggtacc
     2821 gaaaccataa ctgtcccagc aggtacgttt gatgcgacaa aaattcaagc caccttcact
     2881 gccaacatcg atgttgattt cgaaggaagc atcatccggt acacggtcaa cggcacaagt
     2941 ttattatggt atgcaccagg cgtgggcttc gcaaaatcca ttgaaaacat cgatttcagc
     3001 ggcacatcat ttacatccac caccgagctg caaagttata acattccgta aaacaaacgc
     3061 cgcctaataa ggcggcgttt tcattctcca aagaccacgt tcaatttatc gttctcataa
     3121 aaagaccgcg ccgcaaaagg cggggaactt cttttcattg aatggatgcg ctcaagcaag
     3181 accggcagat cctccacgac gggaagttga ctcaccgcct ctttctggac ccattctggt
     3241 gtgccttctt ctgaaccttt cacttcgcct tgagggttct cccctacaac aacaaaaagc
     3301 aaaataccaa tttctcccgc atcgacaatc actgtcccag ccagccaaag gtcggcagaa
     3361 attccagctt cttccatcaa ctcgcgtctt gccgaagaaa gtacatcctc tcctcgatct
     3421 acatgcccac ctaagccatt atatttgcca gcccacaatc gttttgtggg cgcacctttg
     3481 agcaataaat actcatcccc gcgccggacg aaaatcgccg tgcggggaat gagcatatat
     3541 ctatctttcg ttacgccttg ttcagagaca ggcatctttt ttagtctgag agatcgctaa
     3601 cgtcttccgc ggctaccgca tctttaacat aatctgcaag aacgggagca accttcaccg
     3661 gcttgatgaa cttctcggtt tggttcttgg tgtctttcag cccaagcaag ttgaaaagct
     3721 tatcaaactg agcttcccaa gcctttccca acttatccaa agactctgca ggcaaagcgt
     3781 aagactttgc tttgaacggt ccaatagcat tcttacgaac cttgtaatag aactgctcat
     3841 ccgtcatacc aggtttgcgg tcaccagcag cattgacatc caaccaagca taaatttcct
     3901 ttttgaaatc agcggggatg ttatcaaaga acatggtcgc aaaattggtc gcaaggtgaa
     3961 cttcaatcgc ttcgttctga acaaacttat taaagttttc ttccggaacg gttgaagcac
     4021 cgtgctgaac tgtgccaccc atgccataat ccttgcgagc aacgcggcta agatcgcgca
     4081 atacatcaaa ggcgatgctg acttcagcga ttgagccatc gggcagaacc gtgccaccgt
     4141 gtgaagtgcc agtcaacacg ctgatcttgc tcaagccagg ctttccgggg gccaacttct
     4201 gaaggttggc attgtaagca tccatata
//
LOCUS       DAOREF010000144         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12452_length_4220_cov_4.632173, whole genome shotgun sequence.
ACCESSION   DAOREF010000144 DAOREF010000000
VERSION     DAOREF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12452_length_4220_cov_4.632173"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKI33_04150"
     CDS             complement(<1..325)
                     /locus_tag="PKI33_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNM36227.1"
                     /translation="MKPTLSDLERLARGAGAILRDGYNKEHTVHYKGVIDLVTESDHA
                     SEAFLLGEIKSAFPESNILAEESGNIQGNNEDLWYIDPLDGTVNYAHHIPIFCVSIGF
                     STNGVL"
     gene            complement(325..1227)
                     /gene="rocF"
                     /locus_tag="PKI33_04155"
     CDS             complement(325..1227)
                     /gene="rocF"
                     /locus_tag="PKI33_04155"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNM36228.1"
                     /translation="MQIDIIGVPIDLGADRRGVDMGPSAIRYSLLQKKLEDLGYTVND
                     EGNVEVPIAEMCKITNPKLKYIDCIIPMSRRVSGAVSTSIQAGNFPLVIGGDHSLSIG
                     SVRGAARNRKIGVIWVDAHADFNTAETTPSGNIHGMSLAALCGMGDPSLVQLWDEPVP
                     VIDPKKIAIVGARDLDEGEKRNLREAGAMVMGMEQVDRYGMVACLEKAIERISRDVDG
                     IYLSLDLDALDPEHAPGVGTPVPAGLTQREAHLACEMIGETGKLIGMDVVEVNPILDI
                     QNRTALLAVDFALSALGRRIWNGI"
     gene            complement(1264..2916)
                     /locus_tag="PKI33_04160"
     CDS             complement(1264..2916)
                     /locus_tag="PKI33_04160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM36229.1"
                     /translation="MNNSWSIFAGILIFNAITALAIAFILTRRPQKNGIKPLTLMFIS
                     LAFWSFGYAMITLSPQLKDKIFWLKFENFGIQTTALFWFLFAMMYTKMDSWMDNKIVL
                     LALWVPPAVALTFILSDRWFHLYYSSVKIATEAGGPLLIERGPFYIYALVCTYTLYLA
                     GLAALVRRFLVSRDIYRSQTPYLIGAVIIPFSLNIIYQVNPGILPIFPAPVDLTALAF
                     TFTAAFLATSIFGLRLLDLVPIARDNVIESIPEMVFVLDDKERIIDANAVAQQWLEKP
                     FAEILGKEPIEVFQKWPQLLQRFFFMERTREEIEIPGDPPRTLETIITPIYNRTNQLE
                     GRVILAYDITDRKKLEKDLKDTNESLQAQLQENERLRVQLQEQAIRDPLTGVFNRRYL
                     AEALDKETARAVREKSPFSLIVLDVDFFKKVNDTYGHKCGDLVLQSLAKFLQENTRRS
                     DIVCRFGGEEFVILMPDAAVEDAYERAERFRNNFALQVVEHEGKQVKSTFSAGVASFP
                     VHANVSETLLSMADLALYQSKANGRNRVTVFSPEIEEPAPAK"
     gene            complement(2922..4196)
                     /gene="hutI"
                     /locus_tag="PKI33_04165"
     CDS             complement(2922..4196)
                     /gene="hutI"
                     /locus_tag="PKI33_04165"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNM36230.1"
                     /translation="MLIHSASQVLTLSGGPQRGKMLGTLGIIPNGAVVVRDEKIIAVG
                     TTDELRNAYPDEPTLDAGRCVLMPGFVDPHTHLVWAGDRADEFEKKMAGTPYLDILAA
                     GGGILSTVRATRTASLETLMAETRPRMLRMFRNGTTTAEAKTGYGLQTSTELRLLKAL
                     LALNDESPMDLVFTFLGAHAIAPEFKDNPQGYTDEVINTMLPMLKSWWETHAPRHTLP
                     FVDVFCETKAFTLEQARQILTQAMALGFPLKIHADEFDNLGGAALAAELKAASADHLV
                     VTSDADIEALGKSDTVAVALPCTPFGLAEAHYTPAKKLIEADALLAVATDCNPGTSWN
                     ESMQFAIALACRYMKLTPAQAIAAATINAAHAIRKADMIGSIEVGKQADMLILSVNDY
                     RQVGYRYGTNLVKQIIKRGQVYSVDVGYYRTA"
BASE COUNT         1128 a          959 c         1016 g         1117 t
ORIGIN      
        1 tcagcacgcc attggtggag aatccgatag agacgcagaa gatggggatg tgatgagcgt
       61 agttgacggt gccatcgagc gggtcgatat accagaggtc ttcattgttg ccttgaatat
      121 ttccgctttc ttcggcgagg atgttacttt cggggaaagc ggattttatt tcaccaagca
      181 gaaaggcttc agaggcatga tcagattcag taaccagatc aattacccct ttgtaatgaa
      241 ccgtgtgttc tttgttgtat ccatcgcgga gaatggctcc ggctccgcgg gcgagtcgtt
      301 caagatcaga gagtgtaggt ttcatcaaat tccattccaa atgcggcgac caagagcaga
      361 gagggcaaaa tccacagcga gcaaggcggt gcgattttga atatcgagga tgggattaac
      421 ttcaacaaca tccatgccga tgagctttcc ggtttcgccg atcatttcac aagctaaatg
      481 cgcttcacgt tgtgtgagcc ccgcagggac gggtgtacct acgccgggtg cgtgttcggg
      541 gtcgagcgca tcaaggtcaa gagagaggta aattccatct acatcgcggc tgatgcgttc
      601 gattgctttt tcaagacaag ccaccatgcc gtaccgatcg acctgttcca tacccatcac
      661 catggcaccc gcttcacgta agtttcgttt ttcaccttcg tcgaggtcgc gtgcaccaac
      721 gatggcgatc tttttggggt caatcactgg aacaggttca tcccataact gtacgaggct
      781 tgggtcgccc atgccacata gagctgctaa agacatgccg tggatatttc cggatggagt
      841 ggtttcagct gtattgaaat cggcgtgtgc atcgacccag atgacgccga tcttgcgatt
      901 acgtgctgcg ccgcgtacag agccaatgga taaagagtga tcgcctccaa tgactaaggg
      961 aaagttgcca gcctgtatcg aagtggatac tgcgccggaa acgcgtctcg acatggggat
     1021 aatgcagtcg atatatttca actttgggtt ggtgatctta cacatttcgg caattggcac
     1081 ttctacattg ccttcgtcgt tgacggtata gccgaggtct tccaattttt tttgcaacaa
     1141 agaatatcga attgcgctgg gacccatatc cacaccgcgg cgatctgcac ccaagtcgat
     1201 aggcacaccg ataatatcga tttgcatgaa agttctccta aactaaaatg aatttgagga
     1261 attttatttt gccggggcgg gttcttctat ttcaggtgaa aacactgtaa cgcgatttcg
     1321 cccattggct tttgattgat aaagcgctag gtctgccatg ctaagaaggg tctcgcttac
     1381 attggcgtga actgggaacg atgcaactcc ggcagagaag gttgatttta cctgcttccc
     1441 ttcgtgctca acgacttgca aggcaaaatt gtttctaaaa cgttcagcac gttcgtatgc
     1501 atcttctacg gcagcatcgg gcatgagaat tacaaattct tcgccgccaa agcggcatac
     1561 aatatcactg cgacgtgtat tctcttgtaa aaattttgca agggattgaa gaacaagatc
     1621 gccgcattta tgtccgtatg tatcgttaac ttttttgaag aagtctacat cgagaacgat
     1681 cagtgaaaat gggcttttct cgcgaaccgc acgcgcggtt tctttgtcca aagcttcagc
     1741 taaatagcgg cgattgaaaa cgccggtcaa cggatcgcga atagcttgtt cttgaagctg
     1801 aacgcgaagg cgctcatttt cttgcaattg agcctgcaga gattcattcg tatcttttaa
     1861 atctttttca agtttttttc ggtcagtgat gtcgtatgca agaatgactc gaccttcgag
     1921 ttggtttgta cggttataaa tgggggtgat aatggtttca agtgtgcggg gcgggtcgcc
     1981 gggaatttcg atctcttcac gtgtacgttc cataaagaaa aaccgctgaa gtaattgggg
     2041 ccatttctga aagacttcaa ttggttcttt tcctagaatc tctgcaaaag gtttttccaa
     2101 ccattgttga gccacggcat ttgcgtcaat gattctttct ttgtcatcta aaacgaatac
     2161 catttcaggt atagattcaa tcacgttatc gcgtgcaatt ggcacaaggt ctagcagacg
     2221 tagaccaaaa atactggtag caagaaatgc cgctgtgaat gtgaaagcta aagcagttaa
     2281 gtctacagga gcaggaaaga ttggcaaaat accagggttt acttgataaa taatattaag
     2341 agaaaatgga ataataacgg cgccaattaa ataaggtgtt tggcttcggt atatatctcg
     2401 agagactaaa aaacgtcgca ccagtgctgc caaccctgca agataaagcg tatatgtaca
     2461 taccaaggcg taaatataga aaggaccacg ctctataaga agaggtccac cagcttcagt
     2521 agcaattttt acactgctgt aatacaagtg gaaccatcgg tcagacagga taaaggttaa
     2581 tgctacagct gggggcaccc acaatgccaa gagcacaatc ttgttgtcca tccaactatc
     2641 cattttggtg tacatcattg caaacaagaa ccaaaacagt gcagtggttt gaatgccaaa
     2701 attttcaaat ttgagccaaa aaatcttgtc tttcagttgt ggcgataaag tgatcatggc
     2761 atacccaaaa gaccaaaatg ccagcgaaat gaacatcaag gtcaagggtt taatgccatt
     2821 tttttgaggt ctacgtgtga gaataaaagc aatagctaac gccgtaattg cattgaagat
     2881 caaaatgccc gcgaaaatac tccaagagtt attcataata attacgcggt cctatagtag
     2941 ccaacatcca cggagtagac ttgtccacgt ttgatgatct gtttgacgag atttgtccca
     3001 tagcgatagc ctacctgtcg atagtcattg accgataaaa tcaacatatc cgcttgtttg
     3061 cctacttcaa tggacccaat catatctgcc tttcgaatag catgtgcggc attgatcgtt
     3121 gcggctgcaa tggcttgggc aggcgttaat ttcatgtagc ggcatgccag cgcaattgcg
     3181 aattgcattg actcgttcca tgaggtgccg gggttacagt ccgtagccac agctaaaagc
     3241 gcatcggctt cgataagttt ttttgcagga gtatagtgag cctctgccaa cccgaatgga
     3301 gtacatggaa gcgcgactgc gactgtgtct gactttccta gcgcttcaat atccgcatca
     3361 gacgtgacca cgagatggtc agcggaagca gcctttaact ccgctgcaag cgctgctcca
     3421 ccaagattat cgaattcatc tgcatgaatc tttagcggga aacccaatgc cattgcttgc
     3481 gtcagaatct gacgcgcttg ttcaagggtg aaagctttcg tctcacagaa aacatctaca
     3541 aagggcaggg tgtgccgcgg agcatgagtc tcccaccagc ttttgagcat gggcagcatg
     3601 gtgttgatta cttcgtctgt atacccttgc ggattatcct taaattctgg tgcaatagca
     3661 tgagctccca agaaagtaaa aacaagatcc atggggcttt catcattcaa tgctaacaaa
     3721 gccttaagca gacgtaattc tgtagacgtt tgaagcccat acccggtctt tgcttctgca
     3781 gttgtggtgc catttctgaa catcctcaac atgcgcggac gggtttctgc cataagtgtt
     3841 tcaaggctgg cagtgcgtgt ggctcgaacc gtagagagaa tgccaccacc tgcggcgaga
     3901 atatcaaggt acggtgtccc tgccattttt ttctcaaact catctgctcg gtcacctgcc
     3961 caaacaagat gggtgtgcgg gtctacaaag ccaggcatta acacacaccg ccctgcatca
     4021 agagtaggct catcaggata agcgttgcgt aattcatccg ttgttcctac cgcaatgatt
     4081 ttttcatcgc gcacgaccac tgcaccattt ggaataatgc ccaatgtgcc aagcattttt
     4141 ccgcgctggg gcccgccgga gagggtcaat acctgagaag ctgagtgaat aagcatggaa
     4201 ccgccaattt atgggaatat
//
LOCUS       DAOREF010000145         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12478_length_4216_cov_4.391252, whole genome shotgun sequence.
ACCESSION   DAOREF010000145 DAOREF010000000
VERSION     DAOREF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12478_length_4216_cov_4.391252"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKI33_04170"
     CDS             complement(<1..468)
                     /locus_tag="PKI33_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNM36231.1"
                     /translation="MKIKAVFFDFGGVIQRTEYQAPRQQLAQRFGMEYEDIDNVVFNS
                     PTAKQATVGEIPVNKHWDAVAKRLKVDKKEIANVEAEFFAGDLLDLSILECLRSLRPR
                     FVTGLISNAWSDMREYLVRKKIDDAFDHLTISAEIGVAKPEAKIYQLALEQAQV"
     gene            complement(553..933)
                     /locus_tag="PKI33_04175"
     CDS             complement(553..933)
                     /locus_tag="PKI33_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36232.1"
                     /translation="MLTALITMEEKHQNHHDAHCPRCRRANSISRQRMELFFPNWREA
                     AKQAASKPVVDDELPAITSVKTTPLSKAAKAAAAKPAATKTKAPAKAKSAGAKPSTTK
                     KTTPSSTSKTSKTPKTSSKSSKKK"
     gene            complement(1081..2079)
                     /locus_tag="PKI33_04180"
     CDS             complement(1081..2079)
                     /locus_tag="PKI33_04180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36233.1"
                     /translation="MLTEKPLLDLNLKLRNTTRADVNAVADLIYAVCEADGDTTVATS
                     VEELESQWRSEKFNPETDSHVIETSDGRVIAYGEFYNHNRHIDLGADIYIHPEFKGKG
                     LFTPVLAWIETRAYEELKLAEPDLRVFIRSTMDGKDMEARQAHEAAGYTAVRYAWRMD
                     IRLTEAPPTPVFPKGVELRPFDKEAHARLLMDAENEAFSEHWGSHASEFEEWAYRKLE
                     NPDFDPTLWLIAWDGDQIAGFSQNRFRMGVGWVSTLGVRKPWRKTGLGLALLQYSFGE
                     FYRRGMKTVGLGVDASNKTGATRLYQRAGMQIASEFVTFEKEIRPGRDLEDAKQRE"
     gene            complement(2093..2668)
                     /locus_tag="PKI33_04185"
     CDS             complement(2093..2668)
                     /locus_tag="PKI33_04185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNM36234.1"
                     /translation="MFTIETQLLEAQDVRLGPIDLETHPAVESRWTHDAEFMHLMELS
                     PSLPLSPAMVKKQYQAIQREMEIQKDLFYFTIRAREDDRLIGKALIEYVDWSHSNGYL
                     RLGIGEAEARRKGYGSQALTLLLQYAFCELNLYRVTAVVPAYNRGALRLFQKFGFIEE
                     SRRPQALHHADMFWDVIGFGLLNSEYREQCL"
     gene            3018..3443
                     /locus_tag="PKI33_04190"
     CDS             3018..3443
                     /locus_tag="PKI33_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36235.1"
                     /translation="MTVSNTSTQNSILIISQDVDMTRVWKNLFEQKNCRVISETTARD
                     GVHAARTLSPTLIILDLDMPDKDRLDLCRDLRASTNGTLLLINASHKQVDVLEYHRAG
                     VDETVSDSISPMALLVKSLAWLARQDWIVPRKQSAQLWA"
     gene            complement(3484..>4216)
                     /locus_tag="PKI33_04195"
     CDS             complement(3484..>4216)
                     /locus_tag="PKI33_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36236.1"
                     /translation="DPTLKVVIALPPDPGVASYAPTAPNVQFLAVNIPNLTAGGNVSV
                     LAPNTQVELPAFLAGYTLAMMTYEYHIGMLYPEGDPNSQAALNAFSNGMRYYCGICDG
                     IYIDPVSYPAILGVPANEDPGKFGGYANVLINDNKVGGLYIYPTLATDDFLSYVGTQG
                     VYQIGTTMPEPRPGGWLMTITPDTIKAIQTAWPQLIAGQGGQNVQSPLGIADVDTGVL
                     TEAKLRLVQQVLDELIAGRILPSNP"
BASE COUNT         1055 a         1036 c         1047 g         1078 t
ORIGIN      
        1 gacctgtgcc tgctccaacg caagttgata gatcttcgcc tcaggctttg ccacacctat
       61 ctcggcagag atggtcaagt gatcgaaagc atcatcgatc ttcttgcgga cgagatactc
      121 gcgcatgtct gaccaagcgt tgctgatgag tcccgtcaca aaacgcggac gtagcgaacg
      181 caggcactcc aagatcgata aatccaaaag atcgccagcg aaaaattcag cttccacgtt
      241 ggcgatttct ttcttatcaa ccttcaaacg tttggcgacg gcatcccaat gcttgttgac
      301 tgggatctcc cctactgtag cttgcttcgc ggttgggctg ttgaaaacga cattgtcgat
      361 atcttcatat tccatgccaa aacgttgtgc caactgttgg cgcggcgctt gatattcagt
      421 gcgttggatc actccaccaa aatcgaaaaa tacagctttg atcttcatag ttggtctatt
      481 cctgtatatg cggataaaaa aatcccgcag gcggatacac acgcctgcgg gttattcaca
      541 aaaaccagca aattacttct tcttgctaga cttcgaagac gttttcggcg tcttggaagt
      601 tttggaagta gaagatggcg tagtcttttt tgttgtcgat ggcttggcgc cagctgattt
      661 agcttttgcc ggagctttgg ttttggtggc tgccggtttg gcagcagcag ctttcgccgc
      721 tttggagagc ggtgttgttt ttacagaagt aatggcgggc aattcatcat ccaccaccgg
      781 tttggaggct gcctgtttcg cagcttcacg ccaattggga aagaagagtt ccatacgctg
      841 gcgcgaaatg gagttggcac gacgacagcg cgggcagtgc gcatcgtggt ggttctggtg
      901 cttctcttcc atggtgataa gtgcggttaa catctccgca cgacctacgg tgaatggggt
      961 ttgacaataa agacaacgca gatttagcat ggtagtgccc tttcgtggat tgtactcata
     1021 gtaccgcttg attctacacc gaatttttct aggttgcgaa tcaagtttgg gcaaaaatgc
     1081 ttattcgcgc tgttttgcgt cctccaaatc cctgccgggg cggatttcct tctcaaaagt
     1141 gacgaattcg ctggcaattt gcatcccggc acgctggtaa aggcgggttg ccccggtctt
     1201 gttggaggca tctacgccta aaccgaccgt tttcatgccc cttcggtaaa actcaccgaa
     1261 ggagtattgt aagagagcca gccccagccc agttttgcgc caaggcttgc ggacgcccaa
     1321 agtgcttacc catcccactc ccatgcggaa acggttctgt gaaaaaccgg cgatctggtc
     1381 gccgtcccag gcaatcagcc acaaggtggg gtcaaaatct gggttttcca atttgcggta
     1441 tgcccattct tcaaattcag aagcatggct accccaatgc tcgctgaatg cttcattttc
     1501 agcatccatc aaaaggcggg cgtgagcttc tttatcgaac gggcgtagtt caacaccctt
     1561 tgggaagact ggtgtaggcg gggcttcggt gaggcggata tccattcgcc acgcatagcg
     1621 gacggcggta tagccagcgg cttcgtgtgc ctgacgtgct tccatgtctt tgccatccat
     1681 cgtgctacgg ataaaaaccc gcaggtctgg ttcagcaagt ttcagctctt cataggctcg
     1741 tgtttcgatc caagccaaga caggcgtgaa aagccctttg cctttgaatt ctgggtggat
     1801 gtaaatatcg gctcccaggt caatatgtcg attgtggttg taaaactcac cataggcaat
     1861 tactcgcccg tcagacgttt cgatgacgtg gctatcggtt tcggggttga atttttcgct
     1921 acgccactgg ctttccaatt cttctacaga ggtcgcaacg gttgtgtcac catcagcttc
     1981 acacacggca tagatcaagt cggctacagc gttcacatct gcccgggtag tgttgcgcag
     2041 tttcaagtta aggtcaagaa gcggtttttc ggtcaacata ctattttgcc ttttataaac
     2101 attgttctcg atattctgag ttcaataagc caaatccaat cacatcccaa aacatatcag
     2161 cgtggtgcaa agcttggggg cggcgggact cttcgataaa accaaatttc tggaacagac
     2221 ggagagcgcc tcggttataa gcaggaacaa cagccgtcac acgatatagg tttagctcgc
     2281 agaacgcata ttgcaacagc aaggttaaag cttgtgaacc gtagcctttg cggcgtgcct
     2341 ctgcttcacc aatacctaaa cggagataac cattgctatg cgaccagtcc acatattcaa
     2401 tgagggcttt gccgatcaga cggtcatctt cgcgggcgcg gatggtgaaa tagaagaggt
     2461 ctttctgtat ctccatttct ctttgaatgg cttggtattg tttcttgacc atcgcaggtg
     2521 aaaggggcag agagggactc aactccatta agtgcataaa ttcggcgtcg tgcgtccagc
     2581 gggattcgac agcgggatgc gtttcaaggt ctatgggacc taaacgtacg tcctgtgctt
     2641 caagcaactg ggtttcaatc gtaaacatga tttccttctt tactaaataa caaaaaacgg
     2701 tcacaagagg ttctctcccg tgaccgttct aaggcggtta aagaaaaacg accacgggct
     2761 tgacgcgccg tggtcgcgaa ggtttccgtt aaacctaacg acgaacaggc gcactactgc
     2821 tatcaccagc gaaactggca gctgcggaaa tgaagaattg catcatgaac atgggtagtg
     2881 cgctcctttc tttagaattc ctttttcagg taacgcgagt ttatcacgca cgaacaaatc
     2941 cagtcaagta ggacatttag gtgaattaag acccttttca aagttttcaa cgacctagtg
     3001 tttatagaga ggtagccatg acagttagca atacttcaac tcagaacagc atcctgatca
     3061 tctcacaaga tgtagacatg acccgtgttt ggaagaactt attcgaacag aaaaattgcc
     3121 gcgtcattag cgaaaccaca gcgcgagatg gagtacacgc ggcacgcact ctctccccta
     3181 ccctgattat tcttgacttg gatatgccag ataaagatcg gctagacctt tgccgcgact
     3241 tgcgagccag caccaatggc actctgctgc tcatcaatgc cagccacaaa caagtggatg
     3301 tactcgaata tcaccgcgcc ggagtggatg aaaccgtatc ggactcgatc agcccaatgg
     3361 cgttattggt caaatcactg gcatggctgg cacggcaaga ttggatcgtt ccgcgcaaac
     3421 aatcggcgca actttgggca taaaaaaaga cccgcgaaat tcgcgggtct ttttttctca
     3481 attctatggg ttggaaggaa gaattctacc tgcgatcaac tcatctaata cttgctgcac
     3541 aagacgtaac tttgcttctg taagaacgcc agtgtccaca tctgcgatac caagtggaga
     3601 ctgaacgttc tgtccgcctt gcccggcaat caactggggc caagcagttt gaatggcttt
     3661 gatagtatcg ggcgtaatgg tcattagcca gccgccgggg cgaggttctg gcatggtcgt
     3721 cccgatctga taaacaccct gggtacctac ataagagaga aaatcatccg tagcaagagt
     3781 ggggtaaatg tataagccac ccactttgtt atcgttgatc aatacattgg catacccacc
     3841 aaatttaccg gggtcttcat tggcaggtac acccaagata gctgggtaac ttacagggtc
     3901 gatataaata ccatcacaga tgccacagta ataacgcatg ccgttgctga aagcgttcaa
     3961 ggcagcctgt gagttcgggt caccttctgg gtagagcata ccaatatgat attcataggt
     4021 catcatcgcc aaagtatagc ctgccagaaa agcaggtaac tctacttggg tgttcggggc
     4081 aagaacgcta acatttccac ccgcagtgag attggggata tttacagcaa ggaattgcac
     4141 attcggtgca gtcggcgcat aacttgccac accagggtcg ggaggcaaag caatgactac
     4201 tttcagggtt gggtcc
//
LOCUS       DAOREF010000146         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12532_length_4203_cov_4.635246, whole genome shotgun sequence.
ACCESSION   DAOREF010000146 DAOREF010000000
VERSION     DAOREF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12532_length_4203_cov_4.635246"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PKI33_04200"
     CDS             <1..1124
                     /locus_tag="PKI33_04200"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNM36237.1"
                     /translation="RLTKKRLIFLTLFYIVWPLGQIMHWFFFWLDDVLFPAYKNQPIE
                     KPLFILGNFRSGSTFLHRLLSRDNETFTSLTTWDIYLTPSVTQKKITQLVAKVDDKYL
                     GSTLHRALFAFDRATLGKVKIHPISFFQPEEDENIHMHVWDGYFVTFLFPFMDEFPDY
                     IHFDESLSPEHKKRIMTFYKSMLQRHMYATGKKHFVAKNPAFSAKIETLAEFFPDARI
                     LYLARNPLDMLPSTVSWINYARGQFTGPNEGYLYIDEILEMTRYWYYHPLQYIDTHPS
                     PRNLIVNYDDLIQRPEAVIRGFYEQFGYPDKPGLPIIIDQAVKETLTFSSDHSYSYEK
                     MGFTRQGIVERYKDIFERFNFETREEEELVPVKRMELER"
     gene            complement(1171..2151)
                     /gene="pyrB"
                     /locus_tag="PKI33_04205"
     CDS             complement(1171..2151)
                     /gene="pyrB"
                     /locus_tag="PKI33_04205"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNM36238.1"
                     /translation="MPTTSTNPHLPFGENKNADWYGKDIISVKQFSRDDLEYVFGVAH
                     EMRGMVERIGTFDLLKGKILANLFYEPSTRTSSSFTAAMERLGGSVIPINEVKYSSVS
                     KGESLPDTIRTLECYADVIVLRHPETGSAAIAAKAAKKPVINAGDGVGEHPTQALLDT
                     FTIMEELGRLDNLNVTMLGDLKYGRTVHSLARLLSQFSNIKLNYVSPDILKMPKEVME
                     EVGAKGVPQAEYSSLEQVLPETDVLYVTRVQKERFEDPADYEKVKGAYVIDPATMKAA
                     KKEMAVMHPLPRVGEISTDFDDDPRAAYFRQMEYGLYVRMALLAMVLGKA"
     gene            complement(2218..3261)
                     /locus_tag="PKI33_04210"
     CDS             complement(2218..3261)
                     /locus_tag="PKI33_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNM36239.1"
                     /translation="MKSKNLVYIFQALLAALFFGASAPIAKLLLGDIAPIYLASFLYL
                     GSGTGIALTKLTQPKGTEASIQRADVPWLAGAIISGGILAPIVLMNSLQNTPASTASL
                     LLNFEGVGTTLLAALFFKESISRRAWMAIIVITLASIFLSTDFSGGFELSLGALGIIL
                     ACVLWGADNNFTRNISGKDPLVIVAWKGLVAGSFSFILAWSLGNPFPAVMTILGTLLL
                     GFLSYGLSTMLFIRSMRGLGAARTSALYGTAPLAGVALSIFMFGEFPSFFFIIAAILM
                     IGGALLLINEEHSHTHVHTAIVHEHSHSHDDGMHGHSGGEGIHSHGHEHPEEEHDHDH
                     MPDIHHRHGHEDK"
     gene            complement(3258..3602)
                     /locus_tag="PKI33_04215"
     CDS             complement(3258..3602)
                     /locus_tag="PKI33_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YqfO family protein"
                     /protein_id="HNM36240.1"
                     /translation="MYDFTDVKLEIFVPETHVNYVRDALVEVGIGVIGNYDHCFAISQ
                     VQGSFRPLPGSNPFDGKIGEVTYSVESKIEVNCRRELVEEALAAVRRSHPYEEPLINI
                     FPLANHLFTRNV"
     gene            complement(3751..>4203)
                     /locus_tag="PKI33_04220"
     CDS             complement(3751..>4203)
                     /locus_tag="PKI33_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36241.1"
                     /translation="FPARAEAIQKPEMITTVYGLLGATQVDAEKIKTWLNDWKHAFKL
                     ASETPGVDARIHPARLNYYEKSIKALLDSDTPLSALWPVLHTWTLSAKVLSGDHLKFW
                     EQACNSLGLLEGFADKVSGLDHFLDEIEIIFEEIATANGLDVEPSTGL"
BASE COUNT         1174 a         1045 c          929 g         1055 t
ORIGIN      
        1 cccgtctcac caagaaacga ctgatatttt tgaccctttt ctacatcgtc tggccccttg
       61 ggcagattat gcattggttt ttcttctggc tggatgacgt cctcttccct gcctataaaa
      121 atcaacccat tgagaagccg ctctttattt tgggtaactt ccgcagtggt tccacgttct
      181 tgcaccgttt gctttctcgt gacaatgaaa cctttaccag cctgaccacg tgggatattt
      241 atctcacacc gtcggtgacc cagaaaaaaa tcacgcaact tgttgctaaa gtggatgata
      301 aatatttagg aagcacatta caccgtgccc tatttgcatt cgaccgtgcc acgcttggca
      361 aggtaaagat acaccccatc tcattcttcc aacccgaaga agatgaaaac attcatatgc
      421 atgtatggga cggctacttt gtcaccttcc tcttcccatt tatggatgaa ttccccgatt
      481 acattcactt tgatgaatcg ctcagccctg aacataaaaa acgtatcatg acgttttaca
      541 aatccatgtt gcagcgccat atgtatgcaa cgggcaagaa acattttgtg gcgaagaacc
      601 cagcattcag tgccaagatc gaaaccttgg ctgaattctt ccccgatgca cgcattctct
      661 acctggctcg caacccgctc gatatgcttc catcaactgt ttcatggatt aactatgcac
      721 gtggtcaatt tacaggtccc aatgaaggct acctgtatat tgacgaaatc ctcgagatga
      781 cgcgctactg gtactatcac ccccttcaat atatcgacac gcatccttct ccccgcaacc
      841 tgatcgtcaa ctacgacgat ttgatccaac gcccagaagc cgtcattcgc ggattctatg
      901 agcaatttgg ttatcccgat aaacccggtc tgcccatcat cattgaccaa gccgttaaag
      961 agacattgac cttcagcagc gaccattcat attcctatga aaagatgggc tttacccgtc
     1021 aaggaatcgt agaaagatac aaagatatct tcgaacgctt caactttgaa acgcgcgaag
     1081 aggaagaact tgttccagtc aaacgcatgg aattagaacg ataaaacaaa aaacgcggac
     1141 aaaaatgtcc gcgtttttat ttaagctcgt ttaggctttt ccaagcacca tggcaagcaa
     1201 tgccattctc acgtacaaac catattccat ctggcggaag taagcagcgc ggggatcatc
     1261 atcaaaatct gttgagattt cgcccacacg cggaagggga tgcatgaccg ccatttcttt
     1321 ctttgcggct ttcatagtag ctgggtcaat cacataagca ccctttactt tttcgtaatc
     1381 ggcgggatct tcaaaacgtt ctttctgaac acgcgtgaca tagagtacat ccgtttcagg
     1441 caagacctgt tccaaagaag aatattccgc ttgcggcacg cccttagcgc ccacttcttc
     1501 cattacttct ttcggcattt tcaaaatatc tggcgagacg taattgagct tgatgttgct
     1561 gaactgcgag agcaatctcg ccagcgagtg gacagtgcgc ccatatttca aatcaccgag
     1621 catagtgacg ttcaagttat caaggcgacc aagttcttcc ataatggtga atgtatcgag
     1681 caaggcttga gtcgggtgct caccgacccc atcgcctgca ttgatgaccg gtttctttgc
     1741 tgcttttgcc gcaatcgccg cggacccggt ttcggggtga cgcagaacga tcacatcggc
     1801 ataacattcc aaggtgcgga tggtatcggg caagctttca cctttggata ctgatgagta
     1861 tttcacttca ttgatcggaa tcactgaccc acccaaacgc tccatggcag ccgtgaatga
     1921 agatgaagta cgtgtggatg gttcgtagaa gagattagca aggattttcc ctttgagtaa
     1981 gtcaaaggtt ccaatgcgct cgaccattcc gcgcatttca tgcgccacgc caaagacata
     2041 ttccaagtca tcacggctaa attgcttgac ggaaataatg tccttgccgt accaatcggc
     2101 gttcttgttt tcgccaaaag gcaaatgggg gttcgtggaa gttgttggca tttcttagtc
     2161 tcctggttct gataattggt tatcaattcg atatatgagt tgggcatata tcggtattta
     2221 cttgtcttca tgcccatgcc gatggtgaat atctggcata tggtcatggt catgttcttc
     2281 ttcagggtgt tcatgcccat gcgaatggat accttcgccg ccactatgcc catgcatacc
     2341 atcatcatgg ctatggctat gttcatgaac aattgctgta tgaacatggg tatgagaatg
     2401 ctcttcatta ataagaagca atgcgccgcc gatcatcaaa atcgcggcga taatgaaaaa
     2461 gaaagaagga aattcaccaa acataaaaat agataaagca acacctgcca gcggagccgt
     2521 accatacaaa gcactggttc gggcggcgcc aagtccgcgc atggagcgga tgaacagcat
     2581 tgtgctcaat ccgtagctaa gaaaccccaa cagcagcgta ccaagaatgg tcatcaccgc
     2641 agggaatgga tttccaagcg accaagctaa gatgaaagaa aatgaaccag caaccaatcc
     2701 cttccatgct acgatgacga gcgggtcttt gccagaaata ttgcgcgtga agttgttatc
     2761 agcgccccaa agcacacaag caaggatgat acccaaagcg ccaagtgaaa gttcgaaacc
     2821 gcctgaaaaa tcagtagaaa gaaaaatact tgcaagggta ataacaatga tcgccatcca
     2881 tgcgcggcga ctaatcgact ctttgaaaaa taaagcagcc aacaaagtag tccctacacc
     2941 ttcgaaatta agcagtaacg aagccgttga tgcaggcgta ttttgcaaac tattcataag
     3001 gacgattggt gcaagaatac cgccagagat aatcgcaccc gccagccaag gcacatctgc
     3061 acgctgaatg ctggcttcgg ttcctttggg ttgagtaagt tttgtgaggg cgattccggt
     3121 tccgctgccg agatacaaaa aggaagcaag gtaaattggc gcaatatcac ccagcaataa
     3181 tttcgcgatc ggtgcacttg caccaaagaa caaagcggcg agcagggctt gaaaaatata
     3241 aacaaggttc tttgacttca tacattcctc gtaaatagat gattcgccaa cggaaagata
     3301 ttaataagtg gttcttcata aggatgagac ctccgcactg cggcaagcgc ctcttccaca
     3361 agctcacgcc tgcaattcac ttcgatcttt gactctacgc tgtaagtgac ctcccctatt
     3421 ttcccatcaa atggattcga ccccggcaat gggcggaacg agccctgcac ttgtgagatg
     3481 gcaaaacagt ggtcatagtt cccgatcaca ccaatcccaa cttctaccag cgcatcgcga
     3541 acatagttga catgcgtttc agggacgaaa atctcgagtt tgacatcagt aaagtcgtac
     3601 atcgaatatc tccattaaga aaaaagcctg acaattaatt gtcaggcttt tgagaacgat
     3661 gaaagaaaac aaagaatgcg cccgggcggg acttgtctaa taaatggcgg tgcggatacc
     3721 ctataaacat cggcgcgatt atactcgcat ttataaacct gtcgatggtt ctacatccaa
     3781 tccgttggcg gtagcgatct cttcaaaaat aatctcgatc tcatcaagga aatgatcaag
     3841 cccagagact ttgtctgcaa agccttcgag tagcccaagc gagttacagg cttgttccca
     3901 gaacttgaga tggtcaccac tcagcacctt ggcagataag gtccaggtat gcagaacagg
     3961 ccagagcgcg gaaagaggcg tatcactatc cagcagggct ttgatgcttt tctcgtaata
     4021 gttcaagcgt gcggggtgaa tccttgcatc cacgccggga gtttcagatg cgagtttgaa
     4081 ggcgtgcttc caatcgttga gccaggtctt gatcttctcg gcatccactt gagttgcgcc
     4141 gagcaaacca tacacagtag tgatcatttc aggcttttgg atggcttcag cacgggcagg
     4201 gaa
//
LOCUS       DAOREF010000147         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12657_length_4180_cov_4.638788, whole genome shotgun sequence.
ACCESSION   DAOREF010000147 DAOREF010000000
VERSION     DAOREF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12657_length_4180_cov_4.638788"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKI33_04225"
     CDS             complement(<1..603)
                     /locus_tag="PKI33_04225"
                     /inference="COORDINATES: protein motif:HMM:NF023325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HNM36242.1"
                     /translation="MEFLTSPSQKFDLPKRIERLGELATNLWWSWHPEAAHLFGRLDY
                     DLWERLGHNPIRILNEVGRTRLNQASKEKEYLELYDAVFKTFDAYFSKETWSQKTHKE
                     LKKPIAYFSMEYGLHETLPIYSGGLGVLSGDHLKEASDLGLPFIAVGFMYAQGYFSQR
                     ITEDGWQEALNNPLNFDHLPVSLVTKDGRAVTVSFDFPDRK"
     gene            complement(750..1097)
                     /locus_tag="PKI33_04230"
     CDS             complement(750..1097)
                     /locus_tag="PKI33_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36243.1"
                     /translation="MPTKTPSIISTVLTVLSLLVFVIASLFFSLVALNGVSESQGTPA
                     LIASLICNGIGIILAAVVAWKMPAWLIHKFSWKSLWAVLVSLIVSFFLGGGISIVAMF
                     VSILVADILWNAR"
     gene            complement(1117..2043)
                     /locus_tag="PKI33_04235"
     CDS             complement(1117..2043)
                     /locus_tag="PKI33_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36244.1"
                     /translation="MPRRFRMTSLVLMWMLVGCSSLPFVATPTPTALPTSTPTRTPLP
                     TQTPSPTVTPILVTYPFKHVWFKYTVERPAEEANPLRLTEEPLLVIYTDGTMIAARTG
                     DPKVRTRVLNTKEVCDFLNRLDTFGFYTLEDSHSTDETNPLYDFGTKYQAVDEGDFAS
                     LTVNRTDPKSVTFFEPYRKFFARPMRKIMEFVETYNPGALKAYEPDRLVVFVQRGRAE
                     GTDEKVRVRDWTVNTFSLKDAADLPYTFLEGQVAVDVYALLAKYETHIYLENEIEYTV
                     TMRLLFPHEVLNADSTPIAIPTEARLPVNCTP"
     gene            complement(2118..>2624)
                     /locus_tag="PKI33_04240"
     CDS             complement(2118..>2624)
                     /locus_tag="PKI33_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNM36245.1"
                     /translation="NNGHTVQVNYDAGSYIELDGVRYDVAQFHYHAPSEHAVDGKLFA
                     AELHIVHKNADGGLAVVGILLDEGAENAAYQPFIQNLPAEESDVKDAGVTVNAADLLP
                     AVQTTFRYSGSLTTPPCTEGVNWLLMTTPVEISASQIEALASLFEAGNNRPVQPLNDR
                     PLAEDSTP"
     assembly_gap    2625..2724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2725..3046)
                     /locus_tag="PKI33_04245"
     CDS             complement(<2725..3046)
                     /locus_tag="PKI33_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNM36246.1"
                     /translation="MKLYKPTLVLVLAALLVACAPATEAASHASTPHWTYEGEEGPEH
                     WGDLDPSYAACETGTKQSPIDVSNPAEQDLANIAFHYQPSEVNILNNGHTVQVNYDAG
                     SYIEL"
     gene            3317..>4180
                     /locus_tag="PKI33_04250"
     CDS             3317..>4180
                     /locus_tag="PKI33_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36247.1"
                     /translation="MHGYGWFMAMRQGDEKPKVTRELLLRVLNYAKPYWWHIAGMLVT
                     ILLSTGLSLLTPLIFRNMIDVVLPAKDTQRLIWLGLALLFIPALSGGVNVLQRRLNST
                     VGEGVIYDLRSSLFARLQRMSLRFFTNTKTGELMSRLNSDVVGAQNAISNTIVNIVTN
                     LIEAVALFAVMISLEWRLTLISVLILPLFIITAQRLGTVLRDIARQAMDTNAQMNAHM
                     NETLNIGGALLVKLFGRTREEEKRFRERAANVRDIGIRRAVIGSTFFVIFGLVTAVGT
                     ALVYGLGGYYVI"
BASE COUNT         1004 a          982 c         1016 g         1078 t
ORIGIN      
        1 ctttcgatca ggaaaatcga atgaaacggt gacggctctg ccatctttgg tgacaaggga
       61 gacgggcagg tgatcgaagt tgagcgggtt gttgagagct tcctgccagc cgtcttcggt
      121 gatgcgttgg gagaaatatc cctgagcata catgaagccg actgcgatga aaggcaagcc
      181 gaggtctgag gcttctttga gatgatcgcc tgaaagcacg cccaagccac cagaataaat
      241 gggcagggtt tcatgcaagc catattccat cgagaaatag gcaatcggtt ttttcaactc
      301 tttgtgcgtc ttttgtgacc aggtttcttt gctaaagtat gcatcgaaag ttttgaagac
      361 ggcatcatat agttcgagat attctttttc tttagatgcc tgatttaagc gtgtgcgccc
      421 aacttcatta agaatgcgga tggggttatg cccgagtcgt tcccacaggt catagtccaa
      481 acgaccaaag agatgagcgg cttcaggatg ccaggaccac cacaagttgg tggcaagttc
      541 accaagacgt tcgatacgtt ttggcaaatc gaatttttga gagggagaag ttaagaattc
      601 catagtgggc ggatgatacc tcattgtttt tttgcgtcca gcgattgtaa ggcggattag
      661 ttgtttggtc aaactaaatg aacttcggaa ttaaaaatga cggtcacctg taaagatgac
      721 cgtcatttga gcaaaatgtt gattcaggtt taacgggcat tccataatat gtccgctacg
      781 agaatgctga cgaacatggc aacaatagaa atccctccgc caaggaagaa gctgactatt
      841 aaggaaacca gcactgccca aaggctcttc cagctaaatt tatggatcag ccaggcaggc
      901 attttccatg cgacgacggc ggcgaggatg attccgatcc cattacatat cagcgatgcg
      961 atcagcgcgg gcgtgccctg actttctgaa accccattca gagcaaccaa agaaaagaac
     1021 aagctggcga tcacaaacac aagcaagctt agaaccgtta gcacagttga aataatggac
     1081 ggggttttgg taggcatggg tttcctcaaa taaaggttat ggtgtgcaat tcacaggaag
     1141 gcgcgcttcg gtggggatgg caatcggcgt agagtctgca ttaagcactt catgtgggaa
     1201 cagcaaacgc atcgtgactg tgtattctat ttcattctca agatagatgt gggtttcata
     1261 cttcgccagc aatgcataca catccacggc gacttgccct tctagaaatg tatatggtag
     1321 gtctgctgca tccttcaaac tgaacgtatt cactgtccaa tcacgcacgc ggactttttc
     1381 atcggtgcct tcggctcgtc cgcgttgcac gaaaacgacc aaacggtctg gctcatatgc
     1441 cttgagcgcg ccaggattgt atgtttctac aaattccatg atcttgcgca tgggtcttgc
     1501 aaaaaacttt cgataaggct caaagaaagt tacgcttttg gggtccgtcc gattcacagt
     1561 gaggctggca aaatcacctt catccacggc ttgatatttt gtgccaaaat catataaagg
     1621 atttgtctca tctgtcgagt gcgaatcttc cagcgtgtaa aaaccgaagg tatctagccg
     1681 gttgagaaag tcgcagactt ctttcgtgtt gagaacccgc gtgcggactt tcggatcccc
     1741 cgtccgcgct gcgatcatgg tgccatcggt atagatgacg agcagcggct cttccgtcaa
     1801 tctaagcgga ttcgcctcct cagccgggcg ctccactgtg tacttaaacc acacatgctt
     1861 gaacggatac gtgacgagaa taggtgtaac cgtaggcgaa ggcgtctgcg tagggagcgg
     1921 agtccgcgtt ggggtagagg tgggaagcgc tgtaggtgtg ggcgtggcaa caaaaggcaa
     1981 agaactacag ccaaccaaca tccacatcaa aacaagcgag gtcatacgaa atcgtcgagg
     2041 catgaaattt gtcttgaatt atatcccgcc cttaaaagag acagtcacct caaaggtgac
     2101 tgtctcttga attcttgtta cggtgtacta tcttccgcca gcggacggtc gttcaagggt
     2161 tggacgggtc tgttattgcc tgcttcaaat aaagaagcaa gggcttcgat ctggcttgca
     2221 gagatttcca caggtgtagt cataagcaac cagttcacac cttcagtaca aggtggtgtg
     2281 gtaagcgagc cgctataacg gaaagtcgtt tgcacagcag ggagcaaatc tgcggcgttg
     2341 acggtaacgc ctgcatcttt cacgtcactt tcttctgctg ggaggttttg aatgaacggt
     2401 tgataggctg cattttctgc gccttcgtct aataaaatac ctaccactgc taagccgcca
     2461 tctgcattct tgtggacgat atgcaattct gctgcgaaga gtttcccatc tacggcgtgt
     2521 tcactgggcg catgataatg gaattgagcc acatcatagc ggacaccatc taattcaatg
     2581 taactgcccg catcatagtt gacttggacg gtatgtccat tgttnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnctaatt caatgtaact gcccgcatca tagttgactt
     2761 ggacggtatg tccattgttc agaatattga cttcgctcgg ctgataatga aaagcgatat
     2821 tcgccaaatc ctgttcagct gggttggaaa catcgatcgg tgattgcttg gtgcctgttt
     2881 cgcaggctgc atacgatggg tcaaggtcgc cccaatgttc aggaccttct tcgccttcat
     2941 acgtccagtg cggagtggaa gcgtgtgagg ctgcttctgt cgcaggtgcg caggcaacaa
     3001 gtaaggcagc tagtacaagt acgagtgtgg gtttatataa cttcatttga ttaactctct
     3061 cctatttaat ttgatgtatt gtatcggttt gtttgaaaag tttctttgca cctgctttgc
     3121 acttcatcag actttcatta acctccccct ttgtccagtt gccggaatgg tcacttggtc
     3181 ttatagtcac atcattccgt aggggcgacc cgcctgagtg tgacaagcca attttcatgc
     3241 aaggatacat gacgcgccat cacctcacca agatcttgac gagtcacccc taccctaatt
     3301 atcggagaca ccctttatgc acggatacgg ctggttcatg gcgatgcgcc aaggcgacga
     3361 aaaacccaaa gtcacccgcg agcttttatt acgcgttctc aattacgcca aaccttattg
     3421 gtggcacatt gcaggcatgc ttgttactat tttgctcagc acaggtttat cgttgctgac
     3481 cccactcatc tttcgtaaca tgatcgatgt agtcttgccc gccaaagata cccaacgact
     3541 tatttggctg gggctggcgt tactgttcat cccggcgctt agcggtggag ttaacgtcct
     3601 gcagcgtaga cttaactcga cggtaggcga aggcgtaatt tatgacttgc gttcttcgct
     3661 cttcgctcga ttgcagcgca tgtctttacg tttctttacc aacaccaaaa ctggcgaact
     3721 catgtctcgc ctcaacagtg acgtggttgg agcgcagaac gccatcagta ataccattgt
     3781 caacatcgtc accaatctta ttgaagctgt tgctttattc gctgtgatga tttcgctcga
     3841 atggcggctg actctcatca gcgtgcttat cctgccgttg ttcatcatca ccgcgcaacg
     3901 gcttggaacc gttctgcgcg acattgcccg tcaagccatg gacacgaacg cgcagatgaa
     3961 cgcccacatg aacgagactc tcaacatcgg cggcgcattg ctcgtcaaac tttttggtcg
     4021 cacccgcgaa gaagaaaaac gtttccgcga acgtgccgcg aatgttcgcg atattggtat
     4081 tcgccgtgcc gtcatcggct cgaccttttt cgtcatcttc ggtttggtca cagccgtggg
     4141 caccgcgcta gtctatgggc ttggcggcta ctacgtcatt
//
LOCUS       DAOREF010000148         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12725_length_4167_cov_4.576848, whole genome shotgun sequence.
ACCESSION   DAOREF010000148 DAOREF010000000
VERSION     DAOREF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12725_length_4167_cov_4.576848"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="PKI33_04255"
     CDS             <1..1684
                     /locus_tag="PKI33_04255"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM36248.1"
                     /translation="GVLGAEVVILVVSQSSISSKNVEPEWRLALEHKKRIILAVFEAT
                     PLPPELSNFEWVDFRTNYRRGIKELKGLLNYPEQTVTPPVPQKGFRAPLTFWVALLIS
                     LFLVLGTIPLVWTIIVPYIIFPLPWNIYKRNYSLSRVLPILMCLPVLAIYAPAYSNTI
                     FPFINNLFDVSYFLLFVPLGWLMLLLLISPAMQRRARPEAAFTRFANPLSTKGITPTS
                     VSFFIDHASEDGKYAEDLRAVLEKHGHVYARPDLPAEAIFVLLSSYKKETKYDPDQQS
                     VFPILLQAVTDIEVKLQRIQWIDFRKGIKNAKALARLLPEPQKLLKALASPPTGAQEI
                     FPTAVSVLQYFYLFVGLLGGGSILLSILTLVSEIVSYTINLDIFFEIVFLTIQGILLA
                     GTIVLSVRGLRFRRGGISSIYPLIVLLLFQASIYFSNFLSSVRYYSLSSDASISFLNI
                     SFSGIAFLGGLGISAVFIALGWKDLYRWFPRRQSEITNFIERTFLLYTPKDTKALFLH
                     ALFHIVLLFAFVAPWFGAPFGPIVMLSIFFVCLSVNGLILVPIYWFAKRYSR"
     gene            complement(1713..2090)
                     /locus_tag="PKI33_04260"
     CDS             complement(1713..2090)
                     /locus_tag="PKI33_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36249.1"
                     /translation="MTHTVSYNLELSVIEVYVYGDFRLSEGMEILQKTIAILKQSNCR
                     HVVQDIRKANLKFSLVELFMVPKSIQDALTREGLSFQAHRWSVVANDYSSNLKFVENV
                     VNNRGYTMKVFTDFDKALKWLQG"
     gene            2326..3288
                     /locus_tag="PKI33_04265"
     CDS             2326..3288
                     /locus_tag="PKI33_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM36250.1"
                     /translation="MSKTFNPPFYIRSAYAQTFLASNKFGTQWNNPILKNSKEVILNP
                     VGQVRLQGFYSPQPNAKGLVILLHGWEGSVNSAYILRTAKFLYQNGFSVFRLNFRDHG
                     ETHHLNPGIFYAALLDEVFGAVQQVARYEPTLPFYVIGFSMGGNFALRIARKAEESPI
                     PNLKHVIAISPVLDPEKSTYAIDEFPLLRDYFRKKWTDSMRKKQALFPELYQLYDVFS
                     LPTIAEMTERMLQRHSDYPSASAYFRAYAVTGEALAALSTPLTVITAQDDPIIPVSDF
                     HHLKLSSTGELLIQPYGGHNGFLENIFGKAWYQRKIAEILLPLG"
     gene            3347..>4167
                     /locus_tag="PKI33_04270"
     CDS             3347..>4167
                     /locus_tag="PKI33_04270"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM36251.1"
                     /translation="MKTSLMISYSRRQTPFVDRLYEELQKDGYSSIWLDYQSLTPAQP
                     WYQQILDGITGAETLLLVVSKDSIESKNVEPEWRLALEHKKRIVLVIFEAYPLPVELQ
                     GCEWVDFRLNHKASLQKLSNMLGMPPQPVTSPAPQSGFKAPKRFWLAIALSVIVLLSS
                     LPSWWTLIIPFILIPLPWQIYKRDYSFSRVIPTLTVLFPLFQIISVNWFFEEGSALSF
                     LRDIINLDWLPVVGVLAGWLLVGLLLTPVMQRRATPVAARVRFANPLVADIKKPQP"
BASE COUNT         1051 a         1021 c          849 g         1246 t
ORIGIN      
        1 aggcgtatta ggcgcagagg tcgtgatcct tgtcgtttca caatcttcaa tttcttctaa
       61 aaatgtcgag ccagaatggc ggctggcatt ggaacataaa aaacggatca ttcttgcggt
      121 ttttgaggca acaccattgc cgcctgaatt gagcaatttc gaatgggtcg attttcggac
      181 aaattatcga agagggatta aagaacttaa aggcttgcta aattacccag aacaaacagt
      241 cacaccgcct gtacctcaga aaggttttcg agctccgctt accttttggg ttgcattgct
      301 tatcagccta ttccttgtac ttggcacaat cccattggtt tggaccatca ttgtgcctta
      361 tattatcttt cctttaccgt ggaatattta caaaaggaat tattcgcttt ccagggtctt
      421 gccaatattg atgtgccttc ctgtgctggc aatatatgcg ccagcatatt caaatacaat
      481 ttttcctttt atcaataatc tttttgatgt ttcatatttt cttttgtttg tcccactagg
      541 ctggcttatg ctcttgcttt tgatctcacc tgccatgcaa cgacgggcaa gacctgaggc
      601 agcgtttact cgatttgcaa acccactctc cacgaaaggg ataactccaa cgtctgtttc
      661 ttttttcatt gatcatgcct cagaagacgg gaaatatgcg gaggaccttc gggcagttct
      721 ggaaaaacat gggcatgtct atgctagacc cgatctgccc gccgaggcga tttttgtctt
      781 gttatccagt tataaaaaag aaacaaaata tgacccggat caacaaagtg tgtttccaat
      841 tttgttgcag gcagtaacag acattgaagt gaaactacaa agaattcaat ggatcgattt
      901 tagaaaggga ataaaaaatg caaaggctct ggcaagacta ttgccagagc ctcaaaagct
      961 attaaaggct ttggcttccc cgccgacggg ggcacaggag atttttccca ctgctgtgag
     1021 tgtgcttcaa tatttttacc tctttgtcgg tttgttgggc ggaggatcaa ttcttctttc
     1081 tattcttaca ttggttagtg agattgtcag ttatacaata aatttagata tcttttttga
     1141 gattgttttt ttgacgatac aagggatatt gttggcaggc actatcgtac tttctgtacg
     1201 aggattgcgc tttagacgtg gaggaatttc ttccatatat ccattgattg ttcttttgtt
     1261 gtttcaagca agtatttatt tttcaaattt tctttcttct gtaagatatt atagtctttc
     1321 ttctgatgca tctatatcct ttcttaatat ttcattttct ggaattgcat ttttaggcgg
     1381 gctagggatt tctgctgtat ttatagcttt gggttggaag gatctatatc gctggtttcc
     1441 ccgccgccag tcagaaatta ctaattttat agaacgtaca tttttgcttt atacaccaaa
     1501 ggatacaaag gcacttttct tgcatgcgtt gtttcacatt gttttattgt ttgcctttgt
     1561 agcaccatgg tttggtgccc catttggacc catcgtgatg ctttctatat tctttgtttg
     1621 tctatctgta aacggactga tattagtgcc aatttattgg tttgccaaac gatattcaag
     1681 gtaaaccaag gagcactctc aatatacata atttagccct gcagccactt cagggcttta
     1741 tcaaaatccg taaatacctt catggtatat ccgcggttgt tcactacatt ctccacgaac
     1801 ttcaagttcg aactgtagtc attggcaacc accgaccacc tgtgcgcctg aaaagaaagc
     1861 ccttctctcg tcagcgcatc ttgaatagac tttggcacca taaataactc tacgagcgag
     1921 aacttcaaat ttgccttgcg tatatcttgc acaacatggc ggcaatttga ttgtttcaat
     1981 atggctatgg ttttttgaag aatttccatt ccttcactaa gtctaaaatc tccgtacaca
     2041 tacacctcaa tcacagataa ttccaaattg tatgaaaccg tatgcgtcat tgacgactcc
     2101 aaaggcaaga agagtagttg gtgctaagca gagaccacaa gctgcaagat ggtccaaaac
     2161 gaaaccaatg ataaggtttc taccatttaa tagtagactg gcgaaagagc aaatacaggt
     2221 ttcccctgat tctcttgccc tacctcacca acttaacgag aataaaagta gacctaaata
     2281 gtctgattga caatcctgtt ctgaggcaga tacaatccaa actgtatgag taaaaccttc
     2341 aaccctccct tttatatccg cagtgcctac gctcaaacct tccttgccag caacaaattc
     2401 ggcacgcaat ggaacaatcc cattctcaag aactcgaaag aggtcatcct caacccggtg
     2461 gggcaggtgc gcttgcaggg cttttactcg ccacaaccga atgccaaagg tctagtcatt
     2521 cttctgcacg gctgggaggg cagtgtcaac tcggcgtata ttttacgcac cgccaagttt
     2581 ctctaccaaa acggcttctc ggttttccgg ctcaacttcc gcgaccacgg cgagacacat
     2641 cacctaaatc ccggcatctt ttacgccgcg cttctcgatg aagtctttgg cgcggtacaa
     2701 caagttgctc gttacgaacc gacgcttccg ttctatgtga tcggcttttc aatgggcggg
     2761 aacttcgcct tgcgcatcgc ccgcaaagcc gaagagagtc ccatcccgaa tctcaagcac
     2821 gtcatcgcca tcagcccggt gctcgacccc gaaaagtcca cctatgcgat tgatgaattt
     2881 cctctgctgc gcgactactt ccgcaaaaaa tggaccgact ccatgcgcaa gaagcaggca
     2941 ctcttccccg aactttacca gttatatgac gtcttttccc tcccgaccat tgccgagatg
     3001 accgagcgca tgctccaacg tcacagcgac tatccctccg cctctgccta cttccgcgcc
     3061 tatgccgtta ctggagaagc gctcgcggct ctttctacgc cgctcacggt catcaccgcc
     3121 caagacgatc ccatcatccc cgtctccgac tttcaccatc tcaaactatc gtccacgggc
     3181 gagcttctca tccaacccta cggcggtcac aacggctttc tagaaaatat ctttggcaaa
     3241 gcctggtatc aacggaagat cgcggaaatc ttattaccgc ttggttgata cattctctgc
     3301 ttatataata gattaaaacc aattcaatca acggaggaag ccattcatga aaaccagttt
     3361 gatgatcagt tattcacgcc gccagactcc ttttgtagac cgactgtatg aagaactgca
     3421 gaaagatggg tattcttcca tctggttgga ttatcaaagc ttgacccctg cccagccctg
     3481 gtatcagcag atcttagatg gcattacagg ggcagagacc ctattgttgg tcgtttccaa
     3541 agattcgatt gaatcaaaaa atgttgaacc agaatggcgg ctggcacttg agcataagaa
     3601 gcgcatcgtt ttggtaatct ttgaagccta tcccttgcct gtcgaattac aaggttgtga
     3661 gtgggttgat ttccgtttga atcataaagc cagtctccaa aaactatcca acatgttggg
     3721 tatgcctccg caaccagtta catctcccgc accccaaagc ggattcaaag ctcccaagcg
     3781 cttctggctg gcaattgccc tgagtgtaat tgtcctgctt tcttctctgc cttcctggtg
     3841 gacgctcatt attcccttca tccttatccc gttgccgtgg cagatctata aaagagacta
     3901 tagtttctcg cgtgtcatcc ccacgttgac cgttctattt cctttatttc agataatttc
     3961 ggtgaattgg ttttttgaag aaggtagcgc cctttctttc ttacgtgaca taataaattt
     4021 ggattggctg ccagtcgttg gtgttcttgc aggttggctt cttgttggct tactgctcac
     4081 cccagtcatg caacgacgtg ccacacctgt ggcggcgcgt gtccgttttg ccaacccact
     4141 ggtggcagat atcaagaagc cgcagcc
//
LOCUS       DAOREF010000149         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12819_length_4147_cov_4.314516, whole genome shotgun sequence.
ACCESSION   DAOREF010000149 DAOREF010000000
VERSION     DAOREF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12819_length_4147_cov_4.314516"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKI33_04275"
     CDS             complement(<1..641)
                     /locus_tag="PKI33_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNM36252.1"
                     /translation="MKKISTLRSFDLIKVLSDPRRLDILRLLMAAPATLTLLAQTLKQ
                     SPAWVRHHILALEAAGLIEISEIRKTGKVQEKFYHAKADALLLQELILPKSKKPTVIF
                     SGSHDIALENVSEFLAKHVNLLSLSVGSLDGLVNLRQGLCQISGSHLLDENGEYNTPF
                     VKHLFPDRNVEVITLAYRTQGLMIASGNPKGIKKIADIARTDVRFVNRNPGSGT"
     gene            complement(791..2314)
                     /locus_tag="PKI33_04280"
     CDS             complement(791..2314)
                     /locus_tag="PKI33_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNM36253.1"
                     /translation="MLSRVYSCAVVGLEGVIVEVEVDYTNGLPAVIIVGLPDAAVQES
                     RERVQTAVKNAGLNFPRHRIVVNLSPAAIRKEGPAYDLPIALGVVILAGYLPQESIEG
                     AMFIGELSLDGIVRHTRGVLPMAAAARANGFKRIFVPEVDAGEAALIPDLEVYPVQSL
                     AHLYDHLSGRHLIQPYLPSNDSIEPLFTPTDFAEIKGQDHVKRALEVAAAGGHNVLMV
                     GSPGAGKTLLARALPGILPDMSIEESLDVTRIYSVADQLPAGTPLIKHRPFRAPHHTI
                     SHAGLVGGGNIPKPGEISLAHRGVLFLDEFPEFGTRVLEVMRQPMEDKVVTISRAKGS
                     LTFSANFQLIAAMNPCPCGYWGDTQKACTCAPAVVTKYQKRISGPLLDRIDIHIEVPR
                     VDYEKLSGNKLGETSESIRKRVQTARDIQNQRFTNASDIVCNADMRIGEVRQFCQLQP
                     EGQSLMRAAMSQLNLSARAYHRILKLSRTIADLAGSEEIQSPHLAEALQYRPKLMMG"
     gene            complement(2402..2872)
                     /locus_tag="PKI33_04285"
     CDS             complement(2402..2872)
                     /locus_tag="PKI33_04285"
                     /inference="COORDINATES: protein motif:HMM:NF023621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HNM36254.1"
                     /translation="MGLFNNILEKLGLKKKEEEKKAVGAEGAVKAAPAAKPAAPAAKA
                     APAAAAKAAPAQKDDAAAAAPAAPKAIDVVDVVAQLEAKAKGKELNWKVSIVDLLKLL
                     ELDSSREAREELAKELNCPADQMSDSARMNTWLHKEVLRQIAANGGNIPKDLLD"
     gene            3031..3852
                     /locus_tag="PKI33_04290"
     CDS             3031..3852
                     /locus_tag="PKI33_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36255.1"
                     /translation="MEKQNHPFWTRDSSILLGGFFVTIILIVYIWWPLAEEVLSYIDW
                     NGPWWLYMDWLLLGIFLFMSITIVARANIKTDVLIVFVGICGGLAIESWGTQTNLWHY
                     YTAERPPLWIIPAWPIASLSIDRITRFFNWTIQKLETRTQQPVHRSIFPTLYWMTFAS
                     FLTLMLVFVSPTFDKSYTVLATLLCVMLILTPTDYRYALLTFIAGIGLGYFLELWGTT
                     RQCWTYYTFETPPFFAVLAHGMAAVAFWRAGLLVKMVTEKLVGGKWKTDRAMENE"
BASE COUNT          968 a          960 c         1091 g         1128 t
ORIGIN      
        1 gtccctgagc cggggttgcg attcacaaag cggacgtcgg ttctggcgat atctgctatt
       61 ttcttgatgc ctttaggatt cccactggcg atcatcaatc cctgtgtgcg atatgccaaa
      121 gtgataactt ctacattccg atctggaaag agatgtttaa caaacggcgt gttgtattct
      181 ccgttttcat ccagcaaatg ggagccgctt atttggcata atccctgccg taagttgacc
      241 aacccatcca gcgaaccgac tgacaaacta agtaggttga catgcttagc aaggaattca
      301 cttacatttt ccagcgcaat atcatggcta cctgagaaga tgaccgtagg ctttttgctc
      361 ttgggcagaa taagctcttg tagaagaagc gcgtcagctt tggcgtgata gaacttttct
      421 tgcaccttgc cggtctttcg aatttcactg atctcgatca gcccggcagc ctcaagcgcc
      481 aaaatgtgat gccgcaccca agcaggggac tgcttcaagg tctgagcaag aagcgtcagc
      541 gttgcaggag ctgccatcag caggcgcaaa atatccaacc ggcgcgggtc agaaaggact
      601 ttgattaagt caaatgatcg cagagttgag atttttttca ttcaattaaa gaaaatctta
      661 atagtctgca attattctag cacaaataag atgtttcggc gcaaaaagta atttggagat
      721 ttattgtatg gagggctcta tatcaacctg gcatatgtca aacgaagcaa agaaacctac
      781 tagagttgtt ttatcccatc atcaactttg ggcgatattg cagcgcctct gccagatgcg
      841 gggattggat ctcttcgcta cctgccaagt ctgcgatggt gcgggatagt ttgaggatgc
      901 gatgataggc gcgtgctgac aaattgagct gactcatcgc cgctcgcatc aggctctgac
      961 cttcgggctg gagttggcaa aattgcctta cttccccgat acgcatatct gcattgcaga
     1021 caatatcgct agcattggta aagcgttgat tttgaatatc ccgtgcggtt tgaacgcgtt
     1081 tccgaatgga ctcgctggtt tcgccgagtt tgttgccgct gagcttttcg taatcgactc
     1141 tggggacttc gatgtgaatg tcgatgcggt ccagtagcgg accggagatg cgtttttgat
     1201 acttggtcac cacagcaggg gcgcaggtgc aagctttttg tgtatcgccc caatagccac
     1261 atgggcaggg attcatggca gcaattaatt ggaaatttgc tgaaaacgtt aatgaaccct
     1321 tggcgcggct gatggtgacc actttatctt ccatcggctg gcgcattact tcgaggacac
     1381 gggtgccaaa ttcggggaat tcgtcgagga agaggacgcc acgatgggcg agggagattt
     1441 cgccgggttt ggggatgtta ccgccgccaa cgagaccggc atgagatatg gtgtgatgcg
     1501 gcgcacgaaa tggacgatgc ttgatgagcg gcgtccctgc ggggagttgg tctgctacag
     1561 aatagatgcg ggtcacatcc aacgattctt cgatggacat atctggcaaa atgccgggca
     1621 aggcacgcgc aagtaaggtc ttgccggcgc caggcgagcc gaccattaag acgttatgcc
     1681 cgccagcggc agcgacctcg agtgcgcgtt tgacatggtc ttgtccctta atttcggcga
     1741 agtctgtggg agtgaagagc ggctcgatgg agtcgttgga ggggagataa ggctgaatga
     1801 gatgccgacc cgataaatgg tcgtagagat gcgcaagcga ttggactggg tacacttcca
     1861 aatctggaat cagtgccgcc tcgcccgcat ccacttcagg gacgaagatg cgcttgaatc
     1921 cattcgcacg tgcggcggct gccatgggca atacaccacg tgtgtgacgg acaatgccat
     1981 cgagagaaag ttcgccgatg aacatggcac cttcaatgct ttcttgtggc agatacccgg
     2041 caaggatgac gacacccagt gcaatgggta agtcgtaggc gggaccttct ttacgaatag
     2101 cggctgggga gagattgacg acgatgcgat ggcgcggaaa atttagaccg gcatttttga
     2161 cagcggtttg cacacgttcg cggctttctt gcactgccgc atcgggtaag ccgacgatga
     2221 tgactgctgg aagaccgttg gtgtaatcta cttctacttc aacgatcacg ccttcaagtc
     2281 ccaccacggc gcatgagtaa acgcgggaaa gcataatgaa attttatcat taagataaga
     2341 cctgacaggt cttgaaaacc tgtcaggtct gtttgtaaaa cttttttggt tcttgcgtga
     2401 attagtcgag caagtctttc ggaatgttgc caccattggc agcgatctgg cgaaggactt
     2461 ccttgtgcaa ccaggtgttc atacgagcag agtcgctcat ctggtcggca gggcagttga
     2521 gttccttggc gagttcttca cgagcttcgc gactggagtc gagttcgagc agtttgagca
     2581 agtctacaat ggaaaccttc caattgagtt ctttgccctt ggctttggct tcgagctgcg
     2641 caaccacgtc tacaacatcg atggctttag gggcggcagg agctgcagcc gcagcatcat
     2701 ctttctgggc aggggcagct tttgcggcgg cggcaggagc tgcctttgct gccggggcag
     2761 caggcttggc ggcgggagca gccttaacag ctccttcagc gccaacagct ttcttttctt
     2821 cttccttctt tttgagaccg agcttttcga gaatattatt gaagagaccc atagcaaaaa
     2881 atccttttct ctgaatttgg ttgcaggtaa gacatttacc tgtctttaaa aattataacg
     2941 gcatatcatc ggagaaatat taaaatatcg tcaatgtttt agtacatacg gctgattttt
     3001 gtttcaccgt cctgcactat aatctggcga atggaaaagc aaaatcaccc cttttggaca
     3061 cgagactcat ccattttatt gggtgggttt ttcgttacca tcatccttat tgtttatatt
     3121 tggtggccgc tggcagaaga agtcctctcg tatattgatt ggaacggtcc ctggtggtta
     3181 tacatggact ggctattgct cggcattttt ctttttatgt ccatcaccat tgtggcgagg
     3241 gcgaatatca agaccgatgt attaatcgtt tttgtcggca tctgtggcgg gcttgccatt
     3301 gagtcttggg gcactcaaac caacctctgg cattactaca ccgctgaacg cccgcctttg
     3361 tggattatcc ccgcctggcc catcgcgagt ctttccattg atcgtattac ccgtttcttt
     3421 aattggacga ttcaaaaact ggaaactcgg actcaacaac ctgttcatcg ctcgatcttt
     3481 cccactcttt attggatgac cttcgcctct ttcttgacct tgatgctggt ctttgtttcc
     3541 ccaacctttg acaagtccta taccgttctc gccaccctcc tgtgtgtaat gctcatcctc
     3601 acaccgacag attatcgcta tgccttgttg acctttatcg caggcattgg gctgggatac
     3661 ttcctagagc tgtggggcac cacccgtcag tgttggacgt attacacctt tgagactccg
     3721 cctttctttg cggtgttggc acatggtatg gcggctgtcg ctttttggcg ggcggggttg
     3781 ctggtgaaaa tggtgaccga gaagttggtt ggtgggaagt ggaagacaga cagggctatg
     3841 gagaatgagt aaccagtaac gccaaatgag tggatgacga aatccgcgag agttttactc
     3901 ttgcggattt tttattcgcg taggctaata gacaaacttc cagtggggaa tcgctttcag
     3961 aaagccgtat caagataggg tcaagaaaga ggcggaagtg attttattcc tcttgacaaa
     4021 aaatagtaac tcgtataata ttttcaagtt ttgtaattag taactcgtat actattttca
     4081 agtgaggctc ttttgtccgt tcgaaatgcc attttaggtc tgcttgcgca aaaatcccgg
     4141 catgggt
//
LOCUS       DAOREF010000150         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12838_length_4145_cov_4.512714, whole genome shotgun sequence.
ACCESSION   DAOREF010000150 DAOREF010000000
VERSION     DAOREF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12838_length_4145_cov_4.512714"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKI33_04295"
     CDS             <1..638
                     /locus_tag="PKI33_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNM36256.1"
                     /translation="GTRWCDICQSSLKLLDGIVCDVCGLPQDKPGVCSTCLANRPRFH
                     SLRAWTVFEKPIQPALHKLKYRRDFSMGDAIAAAMLPFVQSLNWNIDLVIPIPLGKQR
                     MHERGYNQVAMIAKPLAMGLGLQYSPHALARRKETRSQVGLSREERQKNVHDAFQASG
                     VQGKIALIMDDVSTTGSTLSSGAEALYAAGAKEVYALTVARALSHHDLRHV"
     gene            680..1243
                     /gene="raiA"
                     /locus_tag="PKI33_04300"
     CDS             680..1243
                     /gene="raiA"
                     /locus_tag="PKI33_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNM36257.1"
                     /translation="MSNKVEVQTRHIRLTERIEEYVSKKAGNLDHYLPAIEDARVELS
                     HHQAARDVNDRNVAQITVFGKGFTLRSEERADEALAAFDKALDNMQRQIERHKGKKYH
                     GRGDGRSAAEAVDDIIDDETGQLSPLFAKRKKFVMYPMNEEEALVQMRNLGHDNFFVF
                     YNAETSKINVLYRRRNGSYGLIEPELG"
     gene            complement(1287..2015)
                     /locus_tag="PKI33_04305"
     CDS             complement(1287..2015)
                     /locus_tag="PKI33_04305"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNM36258.1"
                     /translation="MIETFQNLFAPPRHMILLVIAAWLGLALAERRTERHGISKEDLN
                     NITFYGLIAFVIGGRISYITQNISAFTKSPLGIVSINPNLFDPIGAIAVAFIVLIVYG
                     QRKQLNIWNIFDSLTLFFAVLAIGLGLSHLAAGSAFGLPTDLPWGINLWNATRHPTQI
                     YDTLASSIILILLWLFKPSPRPGITFLTYAAFVALAQLILQAFRANYTIIFSGLRQEQ
                     VVAWVGLLVCFILIEVRLKSQEKQ"
     gene            complement(2012..2554)
                     /locus_tag="PKI33_04310"
     CDS             complement(2012..2554)
                     /locus_tag="PKI33_04310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNM36259.1"
                     /translation="MNRTQRIILYILLLIAGASWIVISGQPTGTTTVAAAAPQAGFTA
                     PDFTLKTAEGETYTLSELKGQAVLINVWATWCPPCKAEMPAIEKMYNEYKGQGFNVLA
                     INSTFQDDPLQIKPFTEEYSLTFPILLDETSDISRDYQVRSLPSSYFVNRYGIITEVV
                     IGGPMSEALLRTKIEEALKP"
     gene            2622..3380
                     /locus_tag="PKI33_04315"
     CDS             2622..3380
                     /locus_tag="PKI33_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNM36260.1"
                     /translation="MLFTDMVFVGIDAASSHKSYTYAALDKGMNLVALADGELDDVTT
                     FLGGQSSAIVAINAPSGVNRGLVREKKKDMFKSVKPRAAGFRVAELELRDRGITVSGT
                     PSTISACSAWVQSGFALYRKLEKMGFKKYPTENTPYQILESQPHAAFCVMAEAIPQPK
                     PSLEGKIQRQLLLYEAGVRIKDPMDFFEEITRHKMLKGNWPLDMLYQPEQLDALVAAY
                     TAWLSIHKPEKVSLIGDSTEGQIALPEKNLKEKY"
     gene            3402..>4145
                     /locus_tag="PKI33_04320"
     CDS             3402..>4145
                     /locus_tag="PKI33_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNM36261.1"
                     /translation="MTNLEFQNKAINTLRFLSADGVQKANSGHPGLPMGAAAMAFTIW
                     TRHLRHSPRNPKWMGRDRFILSGGHGSMLLYSLLHLTGYDLSLEDLQSFRQWGSKTPG
                     HPEYGWTPGVEVTTGPLGAGLSTGVGMAIAGAHLAATFNKPGHELIDSYIYAIVTDGD
                     LMEGVASEAASLAGHLSLGKLIYLYDDNKISIDGSTDLSFTEDRAKRFEAYGWHIQRV
                     EDGNDVEAIDAAIKAAKTDPRPSLILCRTI"
BASE COUNT         1102 a          860 c         1067 g         1116 t
ORIGIN      
        1 ctggaacaag atggtgtgat atctgtcaaa gtagcctgaa attgttggat ggtattgttt
       61 gtgatgtatg cggtctaccg caagataagc ctggtgtttg ttccacttgt cttgcgaata
      121 gaccgcgttt tcattctttg agagcttgga ccgtgttcga aaagcctatc cagccagctt
      181 tgcataagtt gaaataccga cgggactttt ctatgggcga tgcgattgcg gcagcaatgt
      241 tgccgtttgt gcaaagctta aattggaata ttgatcttgt gattccaatt ccgttaggaa
      301 aacaaagaat gcatgaaagg ggatataatc aggttgcaat gatcgcaaaa ccacttgcta
      361 tgggtttggg cttgcaatat tcacctcacg ctttggcacg ccgaaaagag acgcgttctc
      421 aagtggggct gagtagggag gagagacaga agaatgttca tgacgctttt caagcatcag
      481 gggtgcaagg aaaaatagct cttattatgg atgacgtttc aacaactggt tcgactcttt
      541 cctctggcgc agaggctctt tatgctgcgg gtgcgaagga agtatatgcg ttgacagtcg
      601 cgcgtgcatt gtctcatcat gatttgagac atgtatagcg tgtgtttgta acttaacaac
      661 ctaataaaag gagtatctta tgtccaacaa agtggaagtt cagacacgtc atattcgctt
      721 gaccgaacgg atcgaagagt atgtttcgaa gaaggctggc aacctcgatc attacttgcc
      781 agctattgaa gatgcgcgcg tggaactttc gcaccaccaa gctgcacgcg atgtcaatga
      841 tcgaaatgtt gcgcagatta ctgtgtttgg aaagggattt acccttcgct cagaagaacg
      901 cgcagacgaa gccttggctg catttgacaa agcattggat aatatgcagc gtcagattga
      961 gaggcataaa gggaagaagt atcatggacg tggcgatgga cgttcggctg ctgaagctgt
     1021 cgacgacatc atcgatgatg agaccgggca actttcaccg ctttttgcaa aacgcaagaa
     1081 atttgttatg tatcctatga atgaggaaga agcgttagtg caaatgcgta atttagggca
     1141 cgataatttc tttgtgtttt acaatgcaga gacgagcaag attaatgttc tgtatcgcag
     1201 gcgcaatgga agctatggtc tgattgagcc tgagcttgga taggctttca ataaataaaa
     1261 gagctgactt tagtcagctc ttttatttat tgtttttctt gggatttcag cctaacttct
     1321 ataagaatga aacatactag taagcccacc catgcaacta cttgttcctg acgaagccca
     1381 gaaaaaataa tagtgtaatt agctcggaaa gcttgcaaga tgagttgtgc taaggctaca
     1441 aatgcagcat aggttaagaa ggtgatgcct gggcgtggac ttggtttgaa gagccagagc
     1501 aagatcaata tgatggaaga ggcgagcgtg tcataaattt gagttgggtg acgtgtggca
     1561 ttccataagt taattcccca cggaagatcg gtagggagtc caaatgcact gcccgcagca
     1621 aggtggctta acccaagccc aatggcgagg acagcgaaaa aaagtgtcag cgagtcaaag
     1681 atattccaaa tgttgagttg cttgcgttga ccatatacta taagaacgat aaatgctaca
     1741 gcaattgcac cgatagggtc gaagagattt gggttaatgg aaacaatgcc aagtggactt
     1801 tttgtaaacg ccgatatgtt ttgggtaata tacgaaatgc gcccaccaat gacaaaagca
     1861 attaatccat agaaggtaat gttattgagg tcttctttgc tgatgccgtg acgttctgta
     1921 cgccgttctg ccaatgcaag accaagccat gcggcgataa cgagcaagat catgtgacgt
     1981 ggcggcgcga ataaattttg gaacgtttcg atcatggttt caaagcctct tcaattttag
     2041 tgcggagcaa agcttcagac atgggaccac cgatcacaac ttcggtgatg atgccatagc
     2101 gattgacaaa atacgatgaa ggcagtgagc ggacttgata atcacggctg atatctgaag
     2161 tttcatctaa taagatggga aaggttaagc tatattcttc agtgaagggt ttgatctgaa
     2221 gcggatcatc ttgaaaggtg ctgttgatcg ctaatacgtt aaagccttga cccttgtatt
     2281 cgttgtacat tttttcaatg gcgggcattt cggctttgca tggcgggcac caagttgccc
     2341 atacattaat gagaaccgcc tgccctttta gttcggagag ggtgtatgtt tcaccttctg
     2401 cggtttttag ggtaaagtct ggggcggtaa accccgcttg aggggcggct gcggcgactg
     2461 tggttgttcc ggttggctga ccggagatta cgatccacga agccccggca ataagcaaaa
     2521 gaatgtagag aatgattcgt tgggttcggt tcatgagatt ttagaagtaa aataggactg
     2581 tttgttttgt attcggtgtg ctcttaagag ataatcttac tatgcttttt acagacatgg
     2641 tttttgttgg tattgacgct gcctcttccc ataagtccta tacgtatgct gcgctggata
     2701 aggggatgaa tcttgtcgct ctggctgatg gcgagctgga cgatgtgacg accttcctgg
     2761 gtgggcaatc ttcagcgatt gttgcgatca atgcgccttc tggcgtaaat cgtgggttgg
     2821 tgcgcgagaa aaagaaagac atgtttaagt cggtgaagcc gcgtgcggct ggttttcgcg
     2881 tggcagaatt ggaattgcga gaccgcggca ttacggtttc tggtactcct tctacaatta
     2941 gcgcttgctc tgcatgggta caatctggtt ttgcgcttta tcgtaaactg gaaaagatgg
     3001 gcttcaagaa atatccaact gaaaatacgc cctatcaaat tttagagtct cagccgcatg
     3061 ctgctttttg tgtcatggct gaggcgatac cgcaaccaaa gccttcattg gaagggaaaa
     3121 ttcaacgtca attgcttctg tatgaagccg gcgttcgtat caaagacccg atggattttt
     3181 ttgaagagat cacacgccat aaaatgctaa aaggcaattg gccgttagat atgctttatc
     3241 agcctgagca attggatgca ttggtggcag cctatacggc ttggctgtcg attcataaac
     3301 ctgaaaaggt ttcccttatt ggagattcta cagaagggca gatcgccctc cctgagaaaa
     3361 atttgaaaga aaaatactaa ccgaatggta aaataccgcc catgacaaac ctcgaatttc
     3421 aaaacaaagc aatcaataca cttcgatttc tttccgctga cggcgtgcaa aaagcgaatt
     3481 ctggtcatcc cggtctcccg atgggagccg ctgcgatggc tttcaccatc tggactcgcc
     3541 atctgagaca tagcccccgt aaccccaagt ggatgggacg tgaccgcttc atcctttcgg
     3601 gtgggcatgg ttcgatgctt ctatactctc tcctccattt gaccgggtac gacctatctt
     3661 tggaagactt gcaaagtttc cgtcagtggg ggagcaaaac cccgggtcac cccgaatacg
     3721 gctggacgcc cggcgtagaa gtaaccacag gtccgcttgg tgcgggtctc tccaccggtg
     3781 tggggatggc aattgctggt gcacatctcg ctgccacttt caacaaaccc ggtcacgaac
     3841 tgattgactc gtacatctat gccatcgtta cagatggtga tttgatggaa ggtgtcgctt
     3901 ctgaagccgc ttcactggct gggcacctct cgctcggaaa acttatttac ctgtacgacg
     3961 ataataaaat ttccatcgat ggctcaacag acctttcctt cacagaagac cgcgccaagc
     4021 gttttgaagc atatggctgg catattcagc gtgtagaaga tggtaatgat gtagaagcga
     4081 ttgatgccgc tatcaaagct gctaaaacag acccgcgccc gtcgttgatc ttgtgccgca
     4141 ccatc
//
LOCUS       DAOREF010000151         4129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12920_length_4129_cov_3.715758, whole genome shotgun sequence.
ACCESSION   DAOREF010000151 DAOREF010000000
VERSION     DAOREF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12920_length_4129_cov_3.715758"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKI33_04325"
     CDS             <1..559
                     /locus_tag="PKI33_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36262.1"
                     /translation="FTTAMLVATLLHWERFSLGTIPFLAWLVLYIVSPFLIPALWIYN
                     RNTDSHEPEESDVTVPTTARLILRLFGGLVLLLVTICYLIPSLIIPIWPWELTPLTAR
                     VLCGWLSVIGTLSLVNSFDPRWTSWRASFEGIFIAYSLIFVAAFMNPAEFKTGIINWY
                     TLFTAGMLIAIVIFYGIMESRRRKR"
     gene            complement(556..4041)
                     /locus_tag="PKI33_04330"
     CDS             complement(556..4041)
                     /locus_tag="PKI33_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM36263.1"
                     /translation="MIQAKPTYQELEQRCLTAESALSAIRSGQADTVLGEQGVLVLRL
                     KETEKALQEAELKYRTLAENSPDIIYIIDLTKNKSGYLNRETLLGYTLEELERPGEFL
                     LSKIHPDDLSAVKAHWQAALAGNMNAEMEYRLQLKDGSWEWLQSRPRILSWDVNGRPT
                     QLLVTLSIITERKQAEKRLRSMLDIAQTMSTSLDTKTTLQQIVDSITQVTGLDSGAIY
                     TLTGNQLYLATTTPALPPEMPDQFRFANLADHPHIQTTLAKGSSVIIPDTEVAELTEA
                     EREICITRGLRSIAYLPLMSARTAIGVLIVASTKKLRTFTDEELLLYHGFSVQAAQTI
                     ENAQLYDSARVHSNELEQQISERKQAEKKLQESEKLYRYAMEVAGTVPYYESYSEKHP
                     AVTYEFIGEGIRQITGYGPEEFNSQIWDSLVEKAIPVEELEGYSLDEAIQRTRSGEFP
                     IWKCEFRLHDRDGKTHWVFEAAVELRDANGNPVGSIGSYQDITIRKQAEEKLRKSEEQ
                     YRLLFEVASDGVVLHLLSTDRIKNRFRRFNQNICQMLGYTAEELAQLSPMDIQQPAEL
                     LKVPGEAEQLERNRELRFEKVFRRKDGSVFPSEVHSSVFELDGELLVLSIIRDISERK
                     QAEAKLRESEEKYRGLMEAIDSVVATIDYNGRFLYLNEVAAKWLGGTPQELVGKTMFD
                     LFPEPMALQQMEGIREVFQENQSKVSESLTYIQGQYRWFHNSLQPIHNELGEVVNVLL
                     HAVDVHELKVVQQELADLNRSLEQRIEERTIEVRQTNLALERALLAKDEFLANMSHEL
                     RTPLNGILGMAEILLEEEYGLLNERQHAYVNLIDSSGHHLLSLINDVLDLSKVEVGKL
                     ELDTEKVFIEDLCQSSMVFVRQLAKKKNVLVNYLPDPAISTLQADARRLKQILVNLLS
                     NAVKFTPEGGKVMLEVHANRDEGIVTFSVTDTGIGISEDNQKRLFQPFTQVDSSLSRK
                     YDGTGLGLALVKRLTELHGGTVALQSEVGRGSCFTVSLPWREAGSTSHKLDGADAGDN
                     HKFAPSAQTIQATVLLVEDNTYNVMAISEYLEAKGYRLVFAVDGLDALAKAAENSPDL
                     ILMDIQMPNMDGLEATRRLRSDSAFASIPIIALTAHAMGGDRERCLAAGATDYLSKPV
                     KLKELESTIRKWLSS"
BASE COUNT         1023 a         1039 c          868 g         1199 t
ORIGIN      
        1 tttcaccact gccatgttgg ttgctacgct tctacattgg gagcgctttt ctctcggcac
       61 tattcccttc cttgcctggt tggtcttata tattgtctct ccgtttttga ttcctgctct
      121 gtggatatac aaccgcaata ctgattcgca tgagccagaa gaatcggatg tgaccgtccc
      181 taccacggcg cgacttattc tccgcctctt tggtgggctt gtcctgcttc tcgttacgat
      241 ctgttatctc atcccaagcc tgatcatccc gatctggccc tgggagttga ctccgctcac
      301 ggcacgggtc ttatgcggtt ggctttcggt gatcggaacc ctttcgttag tgaactcctt
      361 cgaccctcgc tggacgagtt ggcgcgccag tttcgaaggc atcttcattg cttacagctt
      421 gatcttcgtc gccgccttca tgaatccggc agagtttaag acaggcatca ttaactggta
      481 caccctattt acagctggga tgttgattgc catcgttatt ttttatggca tcatggaatc
      541 tcgcagacgc aaacgctaac tcgaaagcca tttacgaata gttgattcaa gttcctttag
      601 ctttacgggt ttactcaagt aatcggtagc acccgctgcg aggcaacgtt cccggtctcc
      661 tcccatcgca tgagccgtaa gggcaatgat aggaatggaa gcaaaggcag agtcagatcg
      721 caagcgacgg gtggcttcca aaccatccat gttgggcatt tgaatatcca tcagaatcaa
      781 atccggagaa ttctccgctg ccttcgctag agcgtccaaa ccatctacag caaataccaa
      841 tctgtagcct ttagcttcga gatactcgct aatcgccatt acgttatagg tattgtcttc
      901 cactaataaa acagtggctt gtatcgtttg agcagaagga gcaaatttat gattgtctcc
      961 ggcatccgca ccatctaatt tatgtgaggt agaaccagcc tcacgccaag gcaaagagac
     1021 ggtgaaacaa cttccacgcc ctacttcact ttgaagagca actgtaccgc catgcaactc
     1081 agttaatcgt ttcaccagtg ccagccctaa gcctgtgcca tcatattttc ggctcaagct
     1141 ggaatccact tgcgtaaacg gctggaatag acgcttttgg ttatcttccg agatgccaat
     1201 ccctgtatcg gtgacagaga aggtgacaat accttcatcc cgattggcat gtacctcaag
     1261 catgaccttc ccgccctctg gcgtaaattt aacggcgttg ctcaataaat taaccaaaat
     1321 ctgtttgagc cgcctggcat cggcttgtaa cgtggaaatt gccgggtcgg gcaggtaatt
     1381 caccagcaca ttctttttct ttgccagttg tcttacgaat accatactag attgacaaag
     1441 gtcttctata aaaacttttt cagtatccag ttctagtttg cctacttcta ctttggacaa
     1501 atcaagaaca tcgttgatca ggctaagcaa gtggtgtcca ctggaatcga tcaagttcac
     1561 atatgcatgc tgacgctcat tgagcaagcc atattcttct tcaagcagaa tttcagccat
     1621 gcccaaaatg ccgttgagcg gagttcgaag ttcgtggctc atgttggcta ggaattcatc
     1681 tttggcaagc aaagcgcgtt ctaatgccaa atttgtctgg cgcacttcaa tcgttctttc
     1741 ctctatacgc tgttcaagag atcgattcaa atctgccaat tcttgctgta cgaccttaag
     1801 ttcatgaaca tcaacggcat gaagcaacac attaactact tcgcccaatt cattatggat
     1861 cggttggagt gagttgtgaa accaacgata ctgcccctga atataggtca gtgattcact
     1921 cactttactc tgattctctt gaaagacctc acgtatgcct tccatttgtt gcaatgccat
     1981 cggctcagga aaaagatcga acatggtctt accgaccagt tcttggggtg tgccacccag
     2041 ccatttggca gcaacctcat tcaagtacag gaatctcccg ttgtaatcaa tcgtagccac
     2101 cacactatcg atcgcttcca tcaagccgcg atatttttct tcactctcgc gtaacttcgc
     2161 ctccgcttgt ttgcgctcag aaatatcacg gatgatggaa agcaccagaa gctcgccatc
     2221 taattcaaag acactggaat ggacttcgga tggaaatacg gaaccatctt tacggcgaaa
     2281 tactttctcg aaccttaatt cacgattccg ctccaactgt tctgcttctc cgggcacctt
     2341 gagaagttct gctggttgtt ggatatccat tggactcaat tgcgccaatt cctcagcggt
     2401 atatcccaac atctggcaaa tattttgatt gaaacgccgg aaccgattct taatgcgatc
     2461 agtacttagc aaatgcagca ccaccccatc actagctact tcaaacaata agcggtactg
     2521 ctcttcactc tttcgtagtt tttcctctgc ttgtttacga atggtgatat cttgataaga
     2581 accgatggac ccgacagggt ttccattggc atctcttaat tccactgccg cttcaaaaac
     2641 ccaatgggtt ttcccatcgc gatcatgcag ccggaattca cacttccaga tcggaaattc
     2701 gccagagcgg gttctttgaa tggcttcatc aagcgaatag ccttcaagtt cctctaccgg
     2761 tattgccttt tccactaacg aatcccaaat ttgtgaattg aattcttcag gtccatagcc
     2821 agtgatctga cgaatccctt ctccaataaa ttcatatgta acggcgggat gcttctcgct
     2881 atatgactcg taataaggca ccgtgccagc cacttccata gcataacgat aaagcttctc
     2941 tgattcctgt agcttcttct cggcttgttt gcgttcagaa atctgctgtt ccaattcatt
     3001 ggaatgaact ctagcagaat catataactg tgcattttca atcgtttggg cagcctgaac
     3061 tgaaaagcca tgataaagaa gtaactcttc atcggtaaat gtgcgtaatt tctttgttga
     3121 agcaacaatc aataccccta ttgctgtacg agcactcata agtggcaagt atgcaatcga
     3181 acgcaaacct ctagtaatgc agatttcacg ctcagcttcg gttagttcgg caacttcggt
     3241 atcaggtatg atgactgaag agccttttgc aagtgtagtt tgaatatggg ggtggtcagc
     3301 cagattggcg aaacgaaatt ggtctggcat ttcgggcggc aaggcaggcg tggtagttgc
     3361 taaatagagt tggttgcctg tcagggtgta gatcgcaccg ctatctaacc ctgtcacttg
     3421 cgtgatgcta tcaacaattt gttgcaaggt cgtttttgta tcaagagagg tggacattgt
     3481 ttgagcaatg tctagcatag aacgtaaacg tttttccgcc tgtttgcgtt cagtgattat
     3541 actaagtgta actaataatt gagtgggcct accattcaca tcccaactca agatgcgcgg
     3601 gcggctctgc aaccattccc aactaccatc tttcaactgc aaacggtatt ccatctctgc
     3661 attcatgttg cctgcaagtg ccgcctgcca atgcgctttc acggcagaaa gatcgtcggg
     3721 atgtattttg gaaagcagaa actcccccgg tctttccaat tcttccaagg tataccccaa
     3781 cagtgtttcg cggttcaaat accccgactt gtttttcgtt agatcaataa tgtaaataat
     3841 atctggagaa ttttctgcca gggtgcgata tttaagttct gcttcttgta aggctttttc
     3901 tgtctctttc aggcgcaaga ccaatactcc ctgttcacca agtacagtat cagcctgtcc
     3961 tgagcggatc gccgataatg cggactcagc tgttaggcag cgttgttcca attcctgata
     4021 tgtcggtttc gcctgaatca aggggcacgc tccgtttctt tgcaaaacca gatcaaaggt
     4081 tcaagacttt ttccaaaact tctttttgac ttaagttgcc gtacacaat
//
LOCUS       DAOREF010000152         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12931_length_4126_cov_3.994105, whole genome shotgun sequence.
ACCESSION   DAOREF010000152 DAOREF010000000
VERSION     DAOREF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12931_length_4126_cov_3.994105"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKI33_04335"
     CDS             complement(<1..480)
                     /locus_tag="PKI33_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36264.1"
                     /translation="MDDYLVNATGLKKSYGANHAVNGVSLHIPPGEIYGLVGADGAGK
                     TTTMRLLVGALLPDAGEISVCGYDVLKQTERARSTIGYLSQRFSMYEDLTVLENIRFF
                     AEIRGLSSTEWLPRSLEILDFVGLEKFKDRRAGQLSGGMKQKLGLASALVTRPRLLLL
                     "
     gene            complement(547..1857)
                     /locus_tag="PKI33_04340"
     CDS             complement(547..1857)
                     /locus_tag="PKI33_04340"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNM36265.1"
                     /translation="MQNKLPPLPVRIVIAVIVLGTLAYFGFQSLKDDTNGSLTASGTI
                     EATIVNVAPETAGKVTDVRADEGESVTKGQSLLSLDASLLTAQQAVASAAVDSATAAL
                     AAAQTKYDQTLEAALAAQAAQRSKDWQASAPSEFDQPNWYIEQDQQVSSAQAELDAAK
                     AEIDAANENLDRVITDLKNSEFLKTEQELADARAAFFIAQQVKDQAASASDNSGLTDA
                     AQDYYDDAVTRLNDAEEAYNDLMSSDEADDVRYARGQVLVAQQRYDAAYARLLTLQTG
                     ENSPAVIAAGKELDKAKSALAQAEASLALVQAQIAKLEVHAPMDGVILTRNVEPGEFV
                     QPGAAALTIGDLSDLTITVYVPENRYGEISLGQTAEVTVDSFPGVTFTATVIQIANKA
                     EFTPRNVQTVEGRSATMFAIKLSVQDAEGRLKPGMPADVIFISK"
     gene            complement(1899..3029)
                     /locus_tag="PKI33_04345"
     CDS             complement(1899..3029)
                     /locus_tag="PKI33_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36266.1"
                     /translation="MFNTRIVSIVRKEFVQIFRDPRSLALIIVMPIIQLFLLGYAATT
                     DVKNVPIAVLDQSQSAQSRELLDAFRAADYFRIDYVVGSTEEYQLLIESGKIRAALVI
                     PPDYDVQVLDGKAQVLFVLDGSDGSVGATALSTARLIGQSYSTKVQTSQFALRGSGSL
                     PTQPVEVRTQVWYNPDFQSAYFMIPGVIGMILSFITTILTATTIVRERERGTIEQLIV
                     TPIRSWELIVGKLLPYVILAFVETFEILIVGHLAFDVPVRGSLLLITLASGLFLMSSL
                     GIGLFASTIANTQQEAMLSVMMFNLPMIFLSGFFFPVQAMPQFLQFVSLAIPLRYYLV
                     VIRALMLKGVGFAAIQSEIISLTIFGIVIMGIAALRFRKRLD"
     gene            complement(3034..3642)
                     /locus_tag="PKI33_04350"
     CDS             complement(3034..3642)
                     /locus_tag="PKI33_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNM36267.1"
                     /translation="MAEETHTKGERTRIQIEDAAIELFIEQGYHATSMRQIADRADLA
                     LGGIYNHFKSKDEIFEAIIVDKHPYKRLLPLILEAEGHTMEDFLGHAARVVIKELTSQ
                     PYYIKLMLIEIAEFNGVHGAVLIKEIGPKLLPVFERIIKTRKNLRLTNPAMLMRTFIG
                     MVVSYMITEMLISNSMLNKMMPKNPIDAYVDIYLHGIVKSEA"
     gene            3780..>4126
                     /locus_tag="PKI33_04355"
     CDS             3780..>4126
                     /locus_tag="PKI33_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36268.1"
                     /translation="MLSPLEQAIRKDLKHNVTAGLWDGSLFGVALGFASFGTILPLFV
                     ATMTQSATLIGLVPAIHSAGWLFPQLFTASHVSRLRQLKRTVLLNTIHERVPFLGFAA
                     VALLLPVVGVNAG"
BASE COUNT         1086 a         1020 c         1009 g         1011 t
ORIGIN      
        1 gagcaagagc aaacgtggac gagtgaccaa tgccgaggca agcccaagct tttgcttcat
       61 gccgccagaa agttgcccgg cgcggcgatc tttgaatttt tccaaaccga caaagtccag
      121 aatctccaac gaacgcggca gccattcggt ggaagaaagc cctcggatct cagcaaagaa
      181 acggatattc tccaacacgg tcaggtcttc atacatcgag aagcgctgcg aaagatagcc
      241 aatggttgaa cgtgctcttt cggtttgctt taacacatca tatccgcaaa ccgaaatttc
      301 tccggcatct ggcagcaacg ccccaaccag taagcgcatt gtggtcgtct tacctgcccc
      361 atctgcaccg acgagtccat aaatttcgcc gggagggata tgcaaagaaa caccattcac
      421 tgcatgatta gcgccgtacg atttttttaa tccagttgca ttaactagat aatcatccat
      481 aatttctacc ttcaactggt ggtttcgata cgctccataa aaaacgcgga gcgactcaac
      541 caccgattat ttactaataa agataacatc cgcaggcata ccgggtttca aacgtccttc
      601 ggcatcttgc acactgagtt tgatcgcgaa catggtggcg ctgcgtcctt ccacagtttg
      661 cacattgcgc ggagtgaact cggctttgtt cgcaatttgg atgacggtgg cagtgaaagt
      721 cacgccgggg aaagagtcca cagtcacttc ggcggtctgc ccaagggaga tctcgccgta
      781 gcgattctct gggacgtaca ctgtgatggt caggtctgag agatcgccga tggtaagagc
      841 tgccgcaccc ggctggacaa attctcccgg ctcgacatta cgggtaagaa tgactccgtc
      901 cattggggcg tggacttcga gtttagcaat ctgtgcttgg accaatgcca ggctggcttc
      961 tgcttgtgcc agtgcagact ttgccttatc caattctttt ccagccgcga tcacggcagg
     1021 agaattttcg ccggtctgta aggtgagcaa gcgggcatag gcggcatcat aacgctgttg
     1081 cgccacgagg acttgtccgc gggcatagcg cacgtcatcg gcttcgtcgc tgctcatcag
     1141 gtcgttatat gcttcttctg catcatttag gcgggtgaca gcatcgtcgt aataatcttg
     1201 ggcggcatcg gttaatccgc tattatctga agcgctggcc gcctgatctt tcacttgttg
     1261 tgcaatgaaa aatgcagcac gagcatcggc aagttcttgt tctgttttta gaaactcaga
     1321 attcttcagg tctgtaatga cacggtccag gttttcgttg gctgcatcaa tttcggcttt
     1381 ggcggcatca agttctgctt gagccgatga aacctgctgg tcttgttcga tgtaccaatt
     1441 gggttggtca aactcacttg gagcagaagc ctgccaatct ttggagcgtt gtgcagcttg
     1501 ggcggcaaga gcggcttcga gcgtttgatc atatttggtt tgggcagcgg caagggcggc
     1561 tgtggcagaa tctactgcgg cagaggcaac ggcttgttgg gcagttaaca ggcttgcgtc
     1621 gagggagaga agcgattgtc ccttcgtcac tgattcgcct tcatcggcgc ggacatccgt
     1681 cactttgcca gcggtttcag gggcgacatt cacaatggtc gcttcgatgg tgcctgaagc
     1741 agtcagactg ccattggtat catctttgag cgattgaaag ccgaagtatg ccagcgtgcc
     1801 gagcacaatc acagcgataa cgatgcggac gggcagaggg ggtagtttat tttgcatgag
     1861 agactccaga attgaaagtt aaaagtacaa gttgaaaatt agtcgaggcg ttttctaaag
     1921 cgcaaggcag caatgcccat aataacgatg ccaaaaatgg taagggaaat gatctcgctt
     1981 tgaatagcag caaagcctac gcccttaagc atgagtgcgc gaatcaccac aaggtaatag
     2041 cgcaatggaa tcgccagaga tacgaactgc aaaaactgcg gcatggcttg cactgggaag
     2101 aaaaagccag agagaaagat catcggcagg ttgaacatca tgaccgacag catggcttct
     2161 tgctgggtgt tggcaatggt ggaagcaaac aagccaatgc ccaaactgga catgagaaac
     2221 aagcccgaag ctagggtgat gagtaaaaga cttccgcgca cgggcacatc aaacgcaaga
     2281 tgcccgacga taagaatttc aaaggtctcc acgaaagcta aaatgacata gggcaacaac
     2341 ttccccacga tcaactccca cgagcggatt ggcgtaacaa tgagctgttc gatcgtgccg
     2401 cgctcgcgtt cacggacgat ggtagtagct gtgagaatgg tggtgatgaa agaaaggatc
     2461 atcccgatca cgccagggat catgaaatat gccgattgaa aatcggggtt gtaccaaact
     2521 tgagtgcgta cttccacagg ttgtgttggt aatgaacccg aaccacgcag tgcgaattgg
     2581 cttgtttgca ccttggtaga gtacgactga ccaatcaacc gcgcagtgga aagtgccgtt
     2641 gcgcctacac tgccatcgga accatctaac acaaaaagga cttgtgcttt gccatctaaa
     2701 acctgaacat cataatcagg cggaatgacc aacgcagcac ggatcttgcc cgactcaatc
     2761 agcaattgat actcttccgt agaacccacc acataatcaa tgcggaagta atcggcggcg
     2821 cgaaaagcat ctaacaattc acgtgattga gcgctttggc tttggtcaag cacagcgata
     2881 ggcacgttct tcacatccgt ggtggcggca taccccagca aaaacagctg aataatgggc
     2941 ataacgatga tcagcgccag tgaacgcggg tcgcggaaaa tctgcacaaa ctctttacga
     3001 acaatcgaaa cgatacgggt attgaacatg gaatcaagcc tcagatttaa caatgccatg
     3061 caggtaaata tcaacatagg catcgatggg atttttaggc atcattttat ttagcatgga
     3121 attcgatatg agcatttctg tgatcatata cgaaaccacc ataccgataa aagttcgcat
     3181 cagcatggct gggttagtca ggcgcagatt cttgcgagtc tttattattc gttcaaagac
     3241 cggtaacaac ttgggtccga tctccttgat caacacagca ccatgcacgc cgttaaactc
     3301 ggcgatctca atcagcatca acttgatgta atacggctga gacgtcagtt ccttgataac
     3361 cacccgcgcc gcatgaccga gaaaatcctc catcgtatgc ccttctgctt ccaaaataag
     3421 cggtaaaagc cttttatatg gatgtttatc aacaattatg gcttcgaata tctcgtcttt
     3481 actcttgaag tggttgtaaa taccgcccaa tgccaaatct gcccgatcgg caatttgacg
     3541 catcgatgta gcatggtagc cctgctcaat gaacaactca attgcagcat cttcaatttg
     3601 aatacgagtg cgctcgcctt tggtatgtgt ttcttctgcc atgacgtctc cagaaaataa
     3661 atgaacgaac gttcgttttt attttagacc aagtaggttt actttgtcaa ggaggaaaaa
     3721 taagtccata cacctccacc ccacaatgta ccaacaacac cataaggtaa aatcacttta
     3781 tgctctcccc acttgaacaa gctattcgca aagaccttaa acacaatgtt actgccgggc
     3841 tatgggatgg aagtctattc ggtgtggcgt tggggtttgc ctccttcgga actatcctgc
     3901 cgctctttgt cgccaccatg acccaatctg caaccttaat cggcttggtg cctgctatcc
     3961 attcggcagg ctggcttttc ccacaactat ttactgccag tcacgtctct cgcttacgtc
     4021 aactaaaacg cactgtgtta ctaaatacca ttcatgaacg tgttcccttt ttagggtttg
     4081 cagcagtagc tctcttactc cccgtcgttg gcgtgaatgc cggttt
//
LOCUS       DAOREF010000153         4124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12942_length_4124_cov_4.502580, whole genome shotgun sequence.
ACCESSION   DAOREF010000153 DAOREF010000000
VERSION     DAOREF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12942_length_4124_cov_4.502580"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKI33_04360"
     CDS             complement(<1..277)
                     /locus_tag="PKI33_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNM36269.1"
                     /translation="MPTSLVTSNSLLAIDVGAANTRAVLFDVIEGEYRFVASGVAPST
                     AEAPYKDVSEGARNAIQALQNVLKRTLLETTARSLILPSQPDGSGVDS"
     gene            complement(283..990)
                     /locus_tag="PKI33_04365"
     CDS             complement(283..990)
                     /locus_tag="PKI33_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36270.1"
                     /translation="MPVLSLDLIAGALSFVFTLMVLSYLIGDNPLFKVAIYLFVGVAS
                     GYVAAVVFWQVLYTKLFLPIGQVLLSGAYTQGVLLIVPFLGVVFILLKAVPGMAGVSR
                     IAMAFLVGAGAAVTIAGALTGTLIPQVNATTNFFDLQAAAARNINMVEVAANGTVLLM
                     GVVTSLAYFHFGANPKADGSTKRFAPINMFAWIGRFFISVTLGAVFAGVYAAALTAFI
                     ERVSSLVFFIKTLLGMP"
     gene            complement(992..3829)
                     /locus_tag="PKI33_04370"
     CDS             complement(992..3829)
                     /locus_tag="PKI33_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36271.1"
                     /translation="MAEMITCPVCGTQNPANQAVCQRCQTPLNNALFQPGQSPVHKDT
                     GELEPLLPQWLKDARQSAKEEGETQQQTQQPPKPEAPAKPAASSTDFLAGLQSQAGDD
                     DDEEIPDWLASITGTPAPKQKPNEVVEEPSGTRWVETGNKSDFQQAEPEAEEVPSWLS
                     GMQQQPSPDEKDELTDWFRSSSSEAPSAQPLSFEQPQQSPTFDSAFNPSASNDTPDWL
                     KQMAANAGEKEPESASDSSFGSSDWFSQPAESNPAPQADPFAQSSDTPDWLHQMQENL
                     DSSAQQQPSASPFAASSGDSDLPDWLNQMQAGAVPAEPAQPAEPFNLSSDTPDWLNQM
                     RSDSTPAESAPSAEPFSFSSDTPDWLSQLPADNAASQQPAADPFNASASADMPDWLSG
                     LGSVPGGENSPFVESTPAESEPFSSNSDLPSWLAPEQDAAKKTDSKPRWLQSDQEVAS
                     GEPAWLASNDKTLVPESEQAADETPVNDVFSDMPDWLKSAAPASTIFDDAPAFETPKA
                     ETPKQQPSSPFESAPAFSEDAFPAQNDALFTEMPDWLSNAMEEPASGGMIPEPIKGGD
                     ALAPDILPSWVEAMRPSDQGMSNIVNAANDATLESRGALAGLSGVLPLGAGFAPTSKP
                     KAYSIKLNASDEQVKHAGILEQILAAETSAVSLTSEKSLGSSRVLRWSIAFVLFIVTI
                     LTSFLGTQIFSTPQAQPNELKLAVAVSQAIPDSAPVLVAVDYEASRAGEIEAAAAPLF
                     DNMLLLKHPRLTFVSSNESGAILAERLMNGPLAFHVQNGVSYLNLGYLSGGQMGIRAF
                     AQNPTSVAPSDVNGQPAWASPVLQNVTALNQFALIILLTDDADAARAWVEQTEDSRGA
                     VPILVVSSAQSSPMIYPYYDSGQVAGIVSGLYGGAIFEQQYNNGRPGLARNYWDAYSI
                     GMLLAMLLLVVGGLVNLGLGVRDHNAMREGK"
     gene            complement(3829..>4124)
                     /locus_tag="PKI33_04375"
     CDS             complement(3829..>4124)
                     /locus_tag="PKI33_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36272.1"
                     /translation="SLMALLAVSLLYAAVRLLRRRADLMGIVFVLTAALLFFGSATLP
                     FGDVPIMSTLVRPWVSQILALGGARGILIGVALGTLTTGLRVLFGIDRPYGGK"
BASE COUNT         1116 a         1017 c         1052 g          939 t
ORIGIN      
        1 gcgaatccac gccggagccg tctggttggc ttggcaaaat gaggctgcga gctgtcgttt
       61 cgagcaatgt tcttttgagc acattttgaa gcgcttgaat ggcattacga gcgccttcag
      121 aaacatcttt atacggggct tcagccgtgc taggagccac accagaagcg acaaaacgat
      181 actcgccttc gatcacgtcg aacaacactg cgcgggtatt cgccgcaccg acatcgatgg
      241 caagaagaga gttactagtg accagtgaag tgggcataat acttagggca tcccaagcaa
      301 ggttttgata aagaagacca aagacgaaac gcgttcaata aaggcagtca gggctgcggc
      361 ataaacgcca gcaaagaccg cacctaaggt aacgctgata aagaagcgac cgatccatgc
      421 aaacatattg atcggggcaa aacgttttgt ggagccatct gctttggggt tcgcaccaaa
      481 atggaaatat gccagtgagg tgacaactcc catcagcaga acggtgccat tcgcagcgac
      541 ttccaccata ttaatgtttc gggcagcagc ggcttgaagg tcgaagaaat tagttgtggc
      601 attaacctgc ggaatcagtg tgccggtcaa cgcccctgca atcgtgaccg ccgcgccagc
      661 ccccacaagg aacgccatgg caattcgaga aacgcctgcc atgcccggca ctgccttcag
      721 taagataaat acaacaccca aaaacggcac aatgagcaaa acaccttggg tgtatgcacc
      781 gcttaacaag acctgtccaa tgggaagaaa aagcttggtg taaagcactt gccaaaacac
      841 cacagcggca acatatccag aagcaacccc aacaaacaga taaatggcaa ctttgaaaag
      901 tggattatca ccaatcaaat aacttaacac catgagtgta aaaacaaagc tcaaagctcc
      961 tgcaatcaaa tcgagagaga gtactggcat cttattttcc ctccctcatg gcattatggt
     1021 cacgcacacc aagtcccaaa ttgaccaaac ctccgaccac taaaagcaac atggcgagca
     1081 acatgccaat gctgtatgca tcccaatagt ttcgcgcaag cccggggcga ccattgttat
     1141 attgttgttc aaagatcgcg ccgccgtata gaccagagac aattcctgcc acttgcccag
     1201 agtcataata cgggtaaatc attggtgacg attgtgcgct ggacacaacc aaaattggta
     1261 ccgcaccacg tgagtcttca gtttgttcca cccaggcacg tgcggcatca gcatcatcgg
     1321 tcagtaagat gatgagagcg aattgattta gggcggttac attctgtaaa acaggcgatg
     1381 cccatgcggg ttgaccattg acatcagacg gcgcaacaga ggtggggttc tgcgcaaatg
     1441 cacggattcc catctgtccg ccagagagat atcctagatt caggtaggaa acgccatttt
     1501 ggacgtgaaa agctaacggt ccgttcatga ggcgttcggc aaggatcgcc cccgactcat
     1561 tgcttgaaac aaaggtcagg cgcggatgtt tcaacaatag catattgtca aacaaaggtg
     1621 ccgcagcagc ttcaatttca cccgcacgag aagcttcata atcgacagcg accaatacag
     1681 gcgcactatc tgggatggct tgagaaactg ccaccgccaa ctttaattca ttgggctgcg
     1741 cttgaggtgt ggagaagatt tgcgtaccga ggaaactggt cagaatggtg acaatgaaaa
     1801 gcacaaaagc aattgaccag cgcaacacgc gtgaggaacc tagagatttt tcagaagtga
     1861 gagaaaccgc agatgtttct gccgcaagga tctgttccaa gatacctgca tgcttaacct
     1921 gttcatcact cgcattcaat ttgatggaat aagcttttgg tttgctggtt ggcgcaaagc
     1981 ctgcccccaa aggcaacaca ccagaaagcc ccgccaatgc gccgcgtgat tcaagtgtgg
     2041 catcatttgc ggcattcaca atgttagaca ttccctggtc agaaggtcgc atcgcttcca
     2101 cccacgaagg cagaatatct ggtgcaagcg catcgccacc tttgatgggt tcagggatca
     2161 tcccgcctga agcgggttct tccattgcat tcgaaagcca atcaggcatt tcggtaaaga
     2221 gcgcatcatt ttgtgcaggg aaagcatctt ctgaaaatgc aggtgcagat tcaaatggag
     2281 aagaaggttg ctgtttaggg gtctctgcct tcggggtttc aaaggcagga gcatcatcaa
     2341 aaattgtcga cgcaggcgca gccgatttca accaatctgg catatcgcta aagacatcat
     2401 tgaccggggt ttcatctgca gcttgttcag attcaggaac aagagtcttg tcattagatg
     2461 ccaaccaagc gggctcccct gaagcaactt cctggtcact ttgcaaccaa cgcggtttgg
     2521 aatccgtttt cttggctgca tcctgctcgg gagccaacca agatggtaag tcactattcg
     2581 acgagaacgg ctcagactca gccggagttg attcaacaaa cggagagttt tctccgccgg
     2641 gcacagatcc caatccgctt agccaatcgg gcatatccgc agaagctgaa gcattgaacg
     2701 ggtctgcagc aggttgctga gatgccgcat tatctgccgg aagttgactg agccaatcag
     2761 gcgtatccga agagaagcta aatggctctg ctgatggagc tgattcggca ggtgttgaat
     2821 cagaccgcat ctgattcaac caatcaggcg tatctgaaga gagattaaac ggctcagcgg
     2881 gttgagcagg ttccgcagga acagcgcctg cttgcatttg attaagccaa tccggtaaat
     2941 ctgaatcacc agatgaagct gcaaacggtg aagccgaagg ttgctgttgt gccgaagaat
     3001 cgagattctc ttgcatttgg tgtaaccaat cgggcgtatc cgaagattgg gcaaatgggt
     3061 cggcttgagg agcggggttt gattctgctg gttggctgaa ccagtctgaa gatccaaacg
     3121 aagaatcgga agctgattcc ggttcttttt ctccggcatt cgcagccatt tgttttagcc
     3181 aatctggcgt gtcgtttgat gcagaaggat taaaagcaga atcaaatgtt gggctctgtt
     3241 gtggctgttc aaaggacaat ggctgagcac ttggcgcttc tgaagacgat gaacggaacc
     3301 aatcggtcag ttcgtctttt tcatcagggc tgggctgttg ctgcatgcct gacaaccagc
     3361 ttggcacttc ttccgcctct ggttcagctt gctggaaatc acttttattg ccagtttcca
     3421 cccagcgagt tccactaggt tcttctacta cttcattggg cttttgtttg ggggctggcg
     3481 tacctgtaat gctagcaagc caatcgggga tctcttcatc gtcgtcgtcc cctgcttgag
     3541 attgcagacc tgctaaaaag tcggtactgg aggcggctgg tttggcaggc gcttctggct
     3601 tgggcggttg ttgagtctgc tgttgagtct cgccttcttc tttagctgat tgtctcgcat
     3661 ctttcaacca ttgcgggaga agtggctcta gctcaccagt atctttgtga actggagatt
     3721 gccccggttg gaataaagca ttattaagcg gcgtttgaca gcgttggcaa actgcttggt
     3781 tggcggggtt ttgcgtacca cagacagggc aggtaatcat ttcagccatt acttcccccc
     3841 gtatggacgg tctatgccaa acaagacacg cagacctgtt gtaagtgtgc caagtgctac
     3901 accgatcaaa ataccgcgcg caccacccaa tgccaaaatc tgagagaccc aggggcgtac
     3961 caaggtgctc ataatgggaa catcaccgaa aggcaatgtg gcagaaccaa agaaaagcaa
     4021 agctgctgtt agaacaaaaa caatacccat caagtctgct cggcggcgta ataagcggac
     4081 agcagcatat aacaatgaaa ctgccagcaa cgccataaga gagg
//
LOCUS       DAOREF010000154         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_12945_length_4123_cov_3.765978, whole genome shotgun sequence.
ACCESSION   DAOREF010000154 DAOREF010000000
VERSION     DAOREF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12945_length_4123_cov_3.765978"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1503)
                     /locus_tag="PKI33_04380"
     CDS             complement(76..1503)
                     /locus_tag="PKI33_04380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNM36273.1"
                     /translation="MFSSDDKEYLVTLFEYAPVALWEQDFSHIKKSFDELRAKGVRSL
                     DAHLVMHPEFLDQCMGKIQVLDVNLQTVAMLKADSKEHLLLNLDKVFRDGMRHHFRDE
                     LRALWNGDVKWSGEGVNYTLEGNPVDIILNWRIMPEYENNWERVLVTTEDITARKRAE
                     QRLHNLFEASPISLWEEDYSAIKKYFDVLRIQGVTSLESYLEEHPEAVSYCVGLINVL
                     DVNQKTLQLFGAESKEHLIANLPRIFRDEMEEHFANELIDLWNGKLSYEREGVNYSLS
                     GEPINILLNFKIMPGYEDDFGWALVSIQDISARKKAEEYLHYLGTHDVMTGLFNRAYF
                     EETIQKLELNRKDPVGIIILDLNYLKKTNDTLGHQAGDKLIRRLAEVMIAAFNNGQIA
                     ARIGGDEFAVILPRTEESEMVDFIKQIHALIEINNKYYREPILSISAGFATSSPGMSL
                     ERVIQLADDAMYRSKAEYHHRRKDD"
     gene            complement(1681..1926)
                     /locus_tag="PKI33_04385"
     CDS             complement(1681..1926)
                     /locus_tag="PKI33_04385"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HNM36274.1"
                     /translation="MLAQLIVLLENKENGLSLSEISRAMNAQPSAVLSMIDLLVKKGK
                     LSEIGPDGKCCSTCGLESECNLLAARGKRYVIAQSLG"
     gene            complement(1931..3613)
                     /gene="feoB"
                     /locus_tag="PKI33_04390"
     CDS             complement(1931..3613)
                     /gene="feoB"
                     /locus_tag="PKI33_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNM36275.1"
                     /translation="MNISQSLFKLDYGGKIEREIAKILTAFEEMKFDTGRYPKRWLAL
                     KLLEADTEILERVQSMPNGEQVIALAKQGADHVQSMLGDDFDLLTADRRYGFINGVVR
                     QSLHRPQMDRITLTDRIDDIVTHKWFGLPVFFAVMYVVFRLVIDVSSPFLDWTDAVIN
                     GPIANGMRFLLNLLPLPAWIHSLVIDGIVAGVGGVLVFVPGLLILYFFLALLEDSGYM
                     SRAAFVMDRFMRIVGLHGKSFIPMILGFGCAVPAIYATRTIASRRDRVLTALLVPLMS
                     CSARLPVYVVFGLAFFGSRAGTVIWAMYALGIVVAMLAGMVFTRTILKPDATSAFVLE
                     LPPYRQPAMKSVLIHMWENTREFVRKAGTMILFTSVVMWFLLNLPWGVTEQRDSYFGK
                     VSNAISPIFIPLGFGSWETGGALVSGFMAKEIVVSTMSQIYLGGEDVEVVEPKTFGEE
                     VIGVGTGFVDATISSEKILLSIIPGVNLIGEEAESEDTALSIALRKQFTPLSAVSLLI
                     FVLLYVPCVATLGAIKHEFGTSWAVTSAVYQTVVAWLMAFIVYQGGLLLGLG"
     gene            complement(3618..>4123)
                     /locus_tag="PKI33_04395"
     CDS             complement(3618..>4123)
                     /locus_tag="PKI33_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNM36276.1"
                     /translation="MADLTIALAGNPNAGKTTIFNALTGLHQHTGNWPGKTVEKKEGE
                     IEHNGKVINIVDLPGTYSLTAYSPEEIIARDYIIESRPDVVINVVDATNLERNLYLTV
                     QILELDVPVVLALNMTDDLQKDGARIDVDQLSQLLGNVPVVQTTANKSKGITELIDKA
                     LRMAKRN"
BASE COUNT         1244 a          994 c          808 g         1077 t
ORIGIN      
        1 cattgctttt ggagtgtacg ccttatttag cggaagctaa ataaaaagtg ccagcgatat
       61 cgctggcact ttttattagt catcttttcg tctatggtga tattcagctt tgcttctata
      121 catagcatca tcggctagtt gtatcactct ctctaaactc atgcctggag aactagttgc
      181 aaaaccagcc gaaatactga gaataggttc gcgataatat ttattattga tttcaattag
      241 agcatggatt tgtttgatga aatcgaccat ctcgctttct tctgtgcgag gcaaaataac
      301 tgcgaattca tcgccaccaa tacgagctgc aatctgtcca ttattaaatg cggcaatcat
      361 cacttcagct aagcgccgaa ttaacttatc gcctgcctga tgccctagtg tatcgtttgt
      421 cttttttaga taattcaaat ctaaaataat gatgccaact gggtctttgc gattcaattc
      481 caatttttgg attgtttctt caaaataagc acggttgaaa agcccggtca ttacgtcatg
      541 agtaccgagg tagtgaagat attcctcagc ctttttacgg gctgagatat cttgaataga
      601 aaccaatgcc caaccaaaat catcttcata ccccggcatg atcttgaaat tcaagaggat
      661 attaatgggt tcaccgctta gtgaataatt gaccccttcg cgttcataag aaagtttgcc
      721 gttccataag tcgatcaact cattagcaaa atgttcttcc atttcgtcac ggaaaatacg
      781 aggcaaattt gcaatcagat gttcttttga ttcggcgcca aacaactgca aagttttttg
      841 gtttacatct agtacattta ttaagccaac gcaataagaa accgcttctg ggtgttcttc
      901 aagatagctt tcaaggctgg taaccccttg tattcgtaat acatcaaaat actttttaat
      961 agcactataa tcttcctccc ataacgaaat gggagaggct tcaaacaaat tatgaagccg
     1021 ttgctcagct cttttgcgag cagtaatatc ttccgtagtg accaacaccc gttcccaatt
     1081 attttcgtac tctggcatga tacgccagtt taaaataata tctaccgggt tgccctccaa
     1141 tgtatagttg accccttctc ccgaccattt tacatccccg ttccataagg cacgcaattc
     1201 atcacgaaaa tgatggcgca ttccatcacg aaaaacttta tctaaattta aaagcaaatg
     1261 ttctttagaa tccgctttaa gcatggcaac ggtttgaagg ttgacatcta aaacttgaat
     1321 tttccccata cactggtcta aaaactctgg gtgcatcacc aaatgagcat ctaatgacct
     1381 aacaccctta gcacgcaact catcaaatga tttcttgata tggctaaaat cctgctccca
     1441 aagagcaacg ggtgcatatt caaacaaggt cactaaatac tccttatcat ctgagctaaa
     1501 cataaaccgc tccaagtcta cttcttaagt gaataagggg attataccta gcagcacatg
     1561 aaattacatt tcgaaaaaag tgcaatttca tgccggttgt tacttgccgg aaactagcca
     1621 gagcgtagta tctctttcca aagctgaaac aggtatggga gaagaaagaa gcgatgcact
     1681 tcatcctaat gattgggcta taacgtatcg ttttccacga gcggcgagta agttgcattc
     1741 cgactccaat ccacaagtag aacagcactt gccatctggt ccaatttctg acagtttacc
     1801 tttcttaacc aacaaatcga tcatcgaaag tacggcggat ggttgagcat tcatggcacg
     1861 actaatctca gaaagactca aaccgttctc tttattttca agaagtacaa ttaattgagc
     1921 aagcattcaa tcatcctagt ccaagtagta aaccgccctg atatacaatg aaagccatca
     1981 accatgcgac cacggtctga taaaccgccg aagtgactgc ccatgatgtt ccaaattcat
     2041 gcttgatggc gcccaaggtg gcaacacaag gcacatataa caacacaaaa attagtaaag
     2101 aaacagcgct aagcggagta aattgtttac gcaaggcaat gctaagcgcc gtatcttcgc
     2161 tttccgcttc ttcaccaatc aaatttacgc cggggatgat gctcaacaaa atcttttctg
     2221 agctaatggt cgcatcgaca aaccctgtgc caacgccgat gacctcttcg ccaaatgtct
     2281 tgggttctac aacttcaaca tcttcgccgc caagatatat ttggctcatc gtgctaacca
     2341 caatttcttt tgccatgaac cccgaaacca atgccccacc tgtttcccaa cttccaaaac
     2401 caagcggaat aaagatcggc gaaattgcat tgctaacttt tccaaaataa gaatctcgtt
     2461 gttcggtcac gccccaaggc aagttgagca ggaaccacat cacaaccgat gtgaaaagaa
     2521 tcatggtgcc tgccttacga acaaattcac gcgtattctc ccacatgtgg atcaacacgc
     2581 tcttcatagc aggttgacga taaggcggca actccaacac aaaggcagag gttgcatcag
     2641 gcttgagaat agtgcgtgta aaaaccattc cagccagcat agcaaccaca atgcccaacg
     2701 catacattgc ccaaatcacg gttccagcgc gtgaaccgaa aaacgccaac ccaaagacca
     2761 cataaaccgg caaccgcgcc gagcaagaca taagcggtac aagcaatgca gtgagcacac
     2821 ggtcacgtcg acttgcaatg gtacgagtag catagatcgc cggaaccgca cacccaaagc
     2881 ccaggatcat tgggataaat gatttgccat gcaaaccaac aatgcgcatg aagcgatcca
     2941 taacaaaggc agcgcgagac atatagccag agtcttcgag caaagcaagg aagaaataca
     3001 aaataagcag acccggcacg aagacaagca cgcctccaac tccggcgacg attccatcaa
     3061 tgaccagtga gtgaatccaa gctgggagcg gaagcaggtt aagcaagaaa cgcatgccat
     3121 tcgcaatggg accgttaatt actgcatccg tccaatccaa gaatggagat gagacatcaa
     3181 tcacgaggcg aaagacaaca tacattacag caaagaaaac cggtaaacca aaccacttat
     3241 gggtgacaat atcatcaatt ctgtccgtaa gtgtgatcct atccatttgc ggacggtgaa
     3301 gcgattggcg cacaacgcca ttgataaatc cataacgcct gtctgcggtg agaaggtcaa
     3361 agtcatcacc aagcatagac tgaacatggt ctgcgccttg tttagcaagt gcaatcactt
     3421 gttccccatt gggcatggat tgaacccgtt ccaatatttc agtgtcggct tcaagaagct
     3481 tgagcgccag ccatcgcttg gggtaacgcc cggtatcgaa cttcatctct tcaaaagccg
     3541 taagtatctt cgcaatttcg cgttcgatct tgccaccata atcaagttta aataaagatt
     3601 gagaaatgtt cataaagtta attccgtttt gccattcgaa gcgctttatc aatcaattca
     3661 gtgatgccct tggatttatt cgctgttgtt tgcactacgg ggacatttcc aagcagttgt
     3721 gagagttgat cgacatcaat acgtgcgcca tctttttgca ggtcatcggt catattaagt
     3781 gccagaacaa caggcacatc caattctagg atctgaaccg tgaggtaaag attacgctcc
     3841 agattggtag catcaaccac gttaatcaca acatctgggc gagattcaat aatgtaatca
     3901 cgggcaataa tttcttcagg agaatacgct gtcaggctgt atgtccctgg caggtcaaca
     3961 atattgatga ctttgccatt atgttcaatt tccccttctt ttttctcaac agtttttccg
     4021 ggccaattgc ctgtatgttg atgcaagccc gtcagcgcat tgaaaatagt ggtcttgcct
     4081 gcattggggt tcccagcaag cgcaatggtc aggtcagcca ttg
//
LOCUS       DAOREF010000155         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13079_length_4096_cov_4.515219, whole genome shotgun sequence.
ACCESSION   DAOREF010000155 DAOREF010000000
VERSION     DAOREF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13079_length_4096_cov_4.515219"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKI33_04400"
     CDS             complement(<1..460)
                     /locus_tag="PKI33_04400"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNM36277.1"
                     /translation="MSTLSPQTQTNIEFAADLIRQSKHMVILTGAGVSTPSGIPDFRS
                     SGTGLWSKDEPLEVASLSTFRTKPSLFFDWFRPLARQMFDAKPNPAHMALSELEQHGY
                     VKSIITQNIDVLHQKAGSKNVIEMHGTLQTLTCTQCYHKEQAEAYIEVFVA"
     gene            complement(457..1620)
                     /locus_tag="PKI33_04405"
     CDS             complement(457..1620)
                     /locus_tag="PKI33_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36278.1"
                     /translation="MAERILIVDDDVDTLRLVGLMLQRQGYQVIAASNGSQGLTKAFE
                     ERPDLILLDVMMPDMDGYEVTRRLRKNPATAAIPVLMFTAKTQLDDKVAGFEVGADDY
                     LTKPTHPTELQSHVKALLARAIPKRPEDMETVYREKNGYVIGVLSVRGGLGVSTVAAN
                     LAAGLYTRSKADVVLAELTPGQGSIAFEFGLSSQNGLSDILKGKSIEITREKIQSALV
                     PHNMGARILAASDSPRDVDLMTYVDNFETLILRLGGLARFIVLDLGSGLPASIQKSLS
                     LCTDRIVVLEGTASSIHQTKILLNEMAELGIDAKTISVVLNNRQRSEAQIPWAQVQER
                     LGHPIVTSLTPASELFMQASKLNTPALVAQPEHMVSQQFFKLADAIIEREKAR"
     gene            1781..2160
                     /gene="ssrA"
                     /locus_tag="PKI33_04410"
     tmRNA           1781..2160
                     /gene="ssrA"
                     /locus_tag="PKI33_04410"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2242..2973
                     /locus_tag="PKI33_04415"
     CDS             2242..2973
                     /locus_tag="PKI33_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36279.1"
                     /translation="MKKLIFIGILLLLSACNLPATADSSSTQAPLTTPETADTTQAVL
                     PTETIAPTATPEPFPQFFTDQFDSESPYWEFMQAGGLSAVTPTFSNRTLHFDIPATDT
                     WMIGIHSANSYENVFVRATASVSPTGSAGLICRYSEDGWYEFNVATDGAYSLLLGQWL
                     SDGVVKYIPVINDVSNKLTELTNTEIGMFCQDNFIQLYVNSTMINRAEVTNYGLTEGK
                     IGISAASFTEAPNSVLFDQVQVSDQ"
     gene            3064..>4096
                     /locus_tag="PKI33_04420"
     CDS             3064..>4096
                     /locus_tag="PKI33_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNM36280.1"
                     /translation="MGKIIGIDLGTTNSVVSVMEGGSPTVITTAEGGRLCPSVVAFNK
                     NGERMVGQTAKRQAVVNADNTIYSIKRFIGRHFDETASERAMVPYQVAQGPTGDVRVK
                     IPVNGKEYSPQEISAMVLSKLKADAEAYLGEPVTQAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTASALAYGLDKKKNETILVFDLGGGTFDVSILEVGEGVIEVKATNG
                     DTHLGGDDWDQSITNWAADEFKKDQGIDLRQDRQALQRLREAAEKAKIELSTVMETEI
                     NLPYITADASGPKHLQLKLSRSKFEQMTEDLLQRCRKPFEAALKDAGMDASKLDEVVL
                     VGGSSRMPMV"
BASE COUNT         1135 a          996 c          930 g         1035 t
ORIGIN      
        1 ttgcaacaaa cacttcaata tacgcttctg cctgctcttt atgataacac tgagtgcaag
       61 tcaaagtttg cagggttcca tgcatttcaa ttacattttt ggatccagct ttttgatgaa
      121 gaacatcaat gttttgggta ataatggatt ttacataccc atgttgttcc aattcagata
      181 gtgccatatg agcagggttg ggttttgcat caaacatttg ccgagccaaa gggcggaacc
      241 aatcaaaaaa taagcttggc ttggtacgaa atgtgcttaa tgatgctact tccaacggct
      301 cgtctttgga ccataagcct gttccagatg aacgaaaatc cggaatgccc gatggtgttg
      361 aaacccctgc cccagtcaga ataaccatgt gtttggattg acgaatcaga tccgctgcaa
      421 attcaatgtt tgtctgagtc tggggagata acgtgctcat cgagcctttt cacgctctat
      481 gatggcatct gctagtttaa agaactgttg cgaaaccata tgttctggct gagcaactaa
      541 cgctggagta ttcaatttgg acgcttgcat gaacagctct gaggctggtg tcagtgatgt
      601 aacaataggg tgccctaagc gttcttgaac ttgcgcccat ggaatttgag cttctgaccg
      661 ttgacgatta ttaagaacca cagaaatggt ctttgcatca atgcccaatt cagccatttc
      721 gtttaagaga atctttgtct ggtgaattga actggctgta ccttcaagaa caacaatacg
      781 atcagtacaa agagaaaggc tcttttgaat tgaagctgga agcccagaac caaggtcaag
      841 aacaataaaa cgagcaagcc cacctaaacg gagaataagg gtctcaaaat tatccacata
      901 ggtcattaaa tctacatcgc gcggactatc tgaagctgcc agaattctag cccccatatt
      961 gtgtggaact aatgcagatt ggatcttttc acgtgtaatt tcaatagact tccccttaag
     1021 gatatctgaa agcccatttt gtgaagacaa cccaaattca aaagcaatag aaccttgccc
     1081 gggtgtaagc tcggctaaaa caacatctgc tttgctgcgg gtgtacaaac ctgctgccaa
     1141 gtttgctgca actgtagaaa cacccaatcc accgcgcaca gaaagaacac caattacata
     1201 cccattcttt tccctgtaaa cagtttccat gtcttcagga cgtttgggta ttgctcgcgc
     1261 aaggagcgct ttaacgtgag attgaagttc ggtggggtgt gtaggcttgg tcagataatc
     1321 atctgcacca acttcaaaac cagctacttt atcatcaagt tgagttttag cagtaaacat
     1381 taaaaccgga atagcagccg tggcagggtt ctttctcaaa cgacgagtga cctcatagcc
     1441 atccatatcc ggcatcatca catctagcag aatcaaatct gggcgttctt caaacgcttt
     1501 tgtcagtcct tgcgagccgt ttgaagctgc aatcacttga tacccttgtc gttggagcat
     1561 taaccccacc aacctcagtg tatctacatc atcatcgaca attaatattc tctctgccat
     1621 gtcaacctca tcgaacaggt gtcttttatt ttgaaacggg acctgaagta gttgctttcc
     1681 tgtttattca attataacgg acaagtaggc aagtttcaca atggggtcat gctataataa
     1741 atatagcaat ggaacgcctg tgtagtcgtt cagcaaagcc ggggatgaca ggcttcgacg
     1801 ggagaggctg gttccggaat gcgagccgag cgcgcaccga actcgtaaaa tcagtgcaac
     1861 ctttaagtgc caacaaccgc acttacgctg ctatgcccct cgccgcctaa ggcgaaccat
     1921 aacagtctga caccttaggt gtcacgtccg ccggccccgt cctccacccg gtgacggatc
     1981 gggcgaaaca atgatggagt gccgcgtgtg atgctgctaa acacgcctaa gccaagcaga
     2041 tggtcacagg tttaccagcc cgccggggtg cctgtggtga cgatatacgg caggctacgc
     2101 tcgtagattt ccgagagtcg aatctttcgg acccgggttc aattcccggc atctccacct
     2161 acatcattgc ccagtaaaca ctgggcaatg attgtgtata aaaccattcc tccctaaata
     2221 acttgtgata taaaatgcaa catgaaaaag ttgattttta ttgggattct cctcttactt
     2281 tctgcctgta atttgccagc aacggcggat tcatcctcta cacaagcgcc tcttaccacg
     2341 ccggaaactg ctgacacaac ccaagcagtt ctgcctacgg aaacgattgc tccaacggca
     2401 acccctgaac catttcctca attttttaca gatcaattcg actctgaatc gccctactgg
     2461 gaatttatgc aagcaggcgg gctctccgcc gtcactccca cgttttcaaa caggacactc
     2521 cactttgata tcccagctac tgatacatgg atgattggta ttcacagtgc caacagctat
     2581 gaaaatgttt tcgtacgtgc tacagcttcc gtttcaccaa caggctctgc cggtttgata
     2641 tgccgatatt ctgaagatgg ctggtatgag tttaatgtcg ctacagatgg agcgtatagc
     2701 cttcttctcg ggcaatggct ttcagatggg gtcgttaaat acattcccgt cattaatgac
     2761 gtcagcaata aattgacaga attaacaaat accgaaatcg gcatgttttg ccaagataac
     2821 tttatacaac tctatgtaaa tagcaccatg attaaccgtg cagaagtaac caactacggt
     2881 ctcactgaag gaaaaattgg aatttcagct gcatcgttta cagaggctcc aaactctgta
     2941 cttttcgacc aggtacaagt tagcgaccaa taagtttatc taaaacctat agattgttaa
     3001 cagaattctt gcactcgtcg cttaatatgg ggacattgaa aaagagtaat aaggagattt
     3061 ctcatgggca aaatcatagg aattgacctc ggcacgacca acagcgtcgt ctccgtgatg
     3121 gaaggcggct ctcccacagt catcaccaca gcagaaggcg gacgtttgtg tccatctgtt
     3181 gtggcgttca acaagaatgg tgagcgtatg gtcgggcaga ccgccaaacg tcaggctgtg
     3241 gtcaatgcag ataacacaat ctattctatt aagcgcttta ttgggcggca ttttgatgag
     3301 accgcctcag agcgcgcgat ggttccttat caagtagcac aaggacccac cggcgatgtg
     3361 cgcgttaaga tccccgtcaa tggcaaggaa tactcgccgc aagaaataag cgcaatggtg
     3421 ttgagcaagc tcaaggcgga tgctgaggcg tatctcggtg aacccgtcac tcaggcggtc
     3481 attaccgtgc ctgcttattt caacgatagc caacgtcaag ccacaaaaga cgcaggcaag
     3541 atcgcaggct tggaagtgtt gcgcatcatc aacgaaccga cggcttccgc gttggcatac
     3601 gggctcgaca aaaagaaaaa cgaaactatc ctcgtcttcg accttggcgg tggtaccttc
     3661 gacgtttcca tcctcgaagt aggcgaaggc gtgatcgaag tgaaagccac caacggtgac
     3721 acacaccttg gcggtgacga ctgggatcag agcattacca attgggctgc cgatgaattc
     3781 aagaaagatc aaggcattga cctgcgccag gatcgccaag cgttacaacg tttgcgcgag
     3841 gcggcagaga aagccaagat cgaactctcg accgtaatgg aaaccgagat caacctgccg
     3901 tacatcacag ccgatgcttc aggtccgaaa catttacagt tgaaacttag ccgctctaaa
     3961 tttgagcaaa tgaccgaaga cctgctgcag cgctgccgta aaccgtttga agcggcactc
     4021 aaagatgccg gcatggatgc tagcaagctc gacgaagttg tcttggttgg tggttcttct
     4081 cgtatgccaa tggttc
//
LOCUS       DAOREF010000156         4095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13085_length_4095_cov_3.047030, whole genome shotgun sequence.
ACCESSION   DAOREF010000156 DAOREF010000000
VERSION     DAOREF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13085_length_4095_cov_3.047030"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1465
                     /locus_tag="PKI33_04425"
     CDS             119..1465
                     /locus_tag="PKI33_04425"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNM36281.1"
                     /translation="MTNIWWIRRDLRLTDNAALHSALEAGSVLPVFILDPAFDNLSER
                     RKHFLYEGLSALDKDLHKRNSYLVVRKGKPIDALRQLFDEVKAETIYAEEDYTPYSRK
                     RDEFIQKLLPLSLVQGQTVHHPKSVLKQDGRPYTVYTPYSKVWKAQLTKIKLHPALEN
                     LNTPKSIRSEPLPAFTPSPIFPAGEAEALRRLNQFTQSIIKNYHDDRNRMDLDGTSSL
                     SPYIRFGMVGLRQAVHATRATHHADSKGAEIWLNELIWREFYIQILYHFPYVAKTAFN
                     RSLANIPWRNKSSEFEAWKRGETGVPVVDAAMRQLKETGWMHNRARMIVASFLVKDLL
                     IDWRWGEDWFMQNLLDGDIAPNNGGWQWTAGTGTDAAPYFRIFNPVLQSAKFDPNGDY
                     IRKWVPELRGLSAKQIHAPWEYDFKISGYPSEPIVDRDVVKERTLNAYSVSKEKIR"
     gene            1462..1989
                     /locus_tag="PKI33_04430"
     CDS             1462..1989
                     /locus_tag="PKI33_04430"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNM36282.1"
                     /translation="MNKRDLSVSMERANIIVLFIGIPVVILQFILFDQLHPSPAETGE
                     WNFLLLLLVIIMGVILHEIVHGVTWAIAGRKPWTSIKFGVQAKTLTPYCHITEPLEIN
                     AYRIGAVMPGLVVGVLPYIYSLMSGNMNWLWFSLVHTSAAGGDWLVLWLIRHVKAGSL
                     VEDHPSQAGCYVLEG"
     gene            1992..2951
                     /locus_tag="PKI33_04435"
     CDS             1992..2951
                     /locus_tag="PKI33_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM36283.1"
                     /translation="MKKRIQKIAGGFFIVILVILLVGPFLIPVPPLENTVTAESLADE
                     DSQFIEVNGLNVHYKTYGTGEPIFILLHGFGASLFSWHEVTAPLAEYGTVIAYDRPAF
                     GLTERPLEWEGESPYSQDAQVELVIGLMDALGIEKATLVGNSAGGTVSMLTALKYPER
                     IESLILVDPAVYAGGGAPAWIRPLLGTPQMRHVGPLISRQLQAQGVEFIKTAWHDPSK
                     IPQSTLEGYQKPLQVENWDKALWELTLASRESGLAERVNEFNLPILVITGDDDRIVPT
                     EQSLRLAEELPNAELAVIPQCGHVPHEECPDDFMQAVKGFLGK"
     gene            2954..>4095
                     /locus_tag="PKI33_04440"
     CDS             2954..>4095
                     /locus_tag="PKI33_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNM36284.1"
                     /translation="MAQQLSPETGRATLKALFHERSPLGPLKVMAKHVGRFFQIPIPG
                     FSPYVVFGAEAARKVLITDRKKLLWRNTDPVTDLLRRGVLVTDGEEHDHYRSLMETPF
                     HPSQLSKYSEMMIQQVDRVISTWQDGQMVDMLVEGRKIALLIIMQTLFNKDAWNDIPK
                     IWKPILKTIQYVSPGMWILWRKIPRLGYGKSLKVIDDYMYAIIDERRKEERKTNDFLQ
                     HLIDAGLSDDIIRDQMLTMLIAGHDTSTALFAWTFALLGQHPEIHENVAQEVDRAEGK
                     YQLLDNVIKEALRLYPPIHIGNRRVAEEMEFAEGKVPAGERMFYSIYLTHRDPEVWEN
                     AEQFCPERFAHGRKTPPMSYIPFGGGPRACIGAAFGQAEARIVLSH"
BASE COUNT         1057 a         1005 c         1020 g         1013 t
ORIGIN      
        1 atcttgacct aaaccgtcag ggctacgtca cgctgaaaat caacgatcaa cccaccgtca
       61 cacacgctct tgtgcgcgtg atgtggttgg atatgatgag cctttctccg caaccactat
      121 gacaaatatt tggtggattc gccgcgacct acgtttgaca gataatgccg cgcttcattc
      181 tgcactcgaa gctggttctg tcttgccggt ctttattctc gacccggcat tcgacaacct
      241 atcagaacgg cgcaaacatt ttctgtatga aggcttatct gctttagaca aagacctgca
      301 taaacgtaat tcatatttag tcgttcgcaa gggcaaaccc atcgacgcac tccgtcaact
      361 tttcgacgaa gtaaaagctg aaaccatcta tgcggaagaa gattacacac cctattctcg
      421 caaacgagac gaatttattc aaaagctgtt gcctttgagt ttggtccaag ggcaaaccgt
      481 acatcaccct aaatcggtcc tcaagcaaga tggtagacct tacacggtgt acacacccta
      541 ctccaaagta tggaaagcac agcttaccaa gatcaaatta caccctgcac tagaaaatct
      601 gaatacaccc aaaagcattc gctctgagcc cttacctgct ttcacgccaa gtcccatttt
      661 cccggcaggc gaagcggaag cgctccgccg ccttaaccaa tttactcaat cgatcatcaa
      721 gaattaccac gacgaccgca accgtatgga cttggatgga acctcatctc tctcaccata
      781 catccgcttt ggcatggttg ggttgcgtca agccgtgcat gctacacgag ctacacatca
      841 cgcagattct aaaggtgcag aaatctggct caatgaactc atctggcgtg agttctacat
      901 tcagatcctg tatcacttcc catatgttgc caaaactgcc ttcaaccgct cactggcgaa
      961 cattccgtgg cgcaataaat cgtcagagtt tgaagcgtgg aaaaggggcg aaacaggcgt
     1021 cccggtggta gatgcggcga tgcgtcagct taaagagaca ggttggatgc ataatcgtgc
     1081 gcgcatgatc gtcgcatcgt tccttgtcaa agacttgctc attgattggc gctggggcga
     1141 agattggttc atgcaaaacc tgctcgatgg cgatatcgca cctaacaacg gcggctggca
     1201 atggacggcg ggaacgggca cagacgccgc gccgtatttt cgtattttta acccggtgct
     1261 gcaaagtgct aagttcgacc cgaacggtga ttacattcgc aaatgggtgc cagagttgcg
     1321 cgggttaagc gcgaagcaaa ttcatgcgcc gtgggaatat gacttcaaga tatcgggtta
     1381 tccatctgaa cccatcgtag atcgagacgt ggtaaaagag cgcacgttaa acgcgtatag
     1441 tgtttcaaag gaaaaaatac gatgaacaaa cgtgatctat ctgtttcgat ggaacgcgca
     1501 aacatcattg tgcttttcat cgggattcct gtagtgattc tacaattcat cctctttgat
     1561 caattacacc catcgcccgc tgaaactggt gaatggaatt tcttgttgtt gctcttggtc
     1621 attattatgg gtgtgatact tcatgagatc gtgcatgggg ttacctgggc aattgcaggg
     1681 cgtaagcctt ggacttcaat taagttcggg gttcaagcta aaacgcttac accgtattgc
     1741 catataactg agccgcttga gattaatgcg taccgtattg gtgcagtcat gccggggtta
     1801 gtcgttgggg ttttgcctta catctatagt cttatgagtg gaaatatgaa ctggctatgg
     1861 ttcagcctcg tccacacctc cgccgctggt ggagactggc ttgtgctctg gctcatccgc
     1921 cacgtaaagg cgggttctct ggtagaagat catcccagcc aggctgggtg ttacgtcctc
     1981 gaaggataaa tatgaaaaaa cgaatccaaa aaattgcggg tggatttttt atcgtaatcc
     2041 ttgttatcct attggttggt ccgttcctca tcccagtacc accgttggaa aacacagtga
     2101 ccgccgaaag tttggcagat gaagatagtc aattcatcga agtcaatggt ttgaatgtgc
     2161 attacaagac ttatggaacg ggcgagccca ttttcatttt gctgcacggc tttggcgcga
     2221 gtttgttttc gtggcacgaa gtgacagctc cacttgcgga gtatggcacg gtcattgcat
     2281 atgaccgccc tgcctttggt ctcactgagc gtccgctaga atgggaaggt gaaagcccat
     2341 acagtcagga tgcgcaagta gaactggtca ttggtttgat ggatgcactt ggcattgaga
     2401 aggcaacctt ggttgggaat tctgcgggcg gaacagtctc tatgttgacg gcgttgaaat
     2461 accccgagcg aattgaaagc ctcattctgg ttgacccagc cgtgtacgca ggtggaggcg
     2521 cgcccgcatg gattcgtcca ctgctgggca caccacagat gcgtcatgtc ggtccgttga
     2581 tctcacgtca acttcaggcg caaggcgtgg aattcatcaa gactgcctgg catgacccaa
     2641 gtaagattcc acagtccacg cttgaggggt atcaaaaacc gttacaagtg gaaaactggg
     2701 acaaagcctt atgggagttg acactcgcca gccgcgaaag cggattggca gaacgggtta
     2761 acgagttcaa tttgcccatc ctggttatca ctggcgatga tgatcgcatc gttccaacgg
     2821 aacagtcttt acgacttgca gaagagctac ccaatgcaga actggctgtc atccctcaat
     2881 gcgggcatgt gccgcacgaa gaatgcccag acgatttcat gcaagcagtg aaaggctttc
     2941 ttggaaagta atcatggcac agcaactttc tcctgaaacc ggtcgcgcca ctttgaaagc
     3001 gctcttccac gaacgctcgc cattaggtcc attgaaagtg atggcaaagc atgtggggcg
     3061 cttctttcag attcccatcc ccgggttcag tccatatgtc gtcttcggag cggaagcggc
     3121 acgcaaggta ttaatcactg atcggaagaa gttgctatgg cgcaacaccg acccagtgac
     3181 cgacctgctg cggcgcggcg tactggtgac cgacggcgaa gagcatgacc actatcgaag
     3241 tctgatggaa acgccattcc atccctcaca gttatcgaaa tattccgaga tgatgattca
     3301 acaagtggat cgagttatat ccacctggca agatggtcaa atggtagata tgttagtgga
     3361 agggcgaaag attgctctgc tcatcatcat gcagaccttg ttcaacaaag atgcctggaa
     3421 tgacattccg aagatctgga aaccaatcct caaaaccatt caatacgtct caccgggaat
     3481 gtggatttta tggcgaaaga ttccgcgtct tgggtacggg aagtcgttga aggtgatcga
     3541 tgattatatg tatgcgatca tcgatgaaag aaggaaagag gaaagaaaaa caaatgattt
     3601 tctgcaacat ttgatcgatg ccgggttgag tgatgacatc atccgcgacc aaatgctgac
     3661 catgcttatt gccgggcatg atacctccac cgccttattt gcatggacct ttgccttgct
     3721 tgggcaacat cctgaaattc acgaaaatgt ggcgcaggaa gttgaccgcg cagaaggcaa
     3781 atatcaacta ttagataacg tcatcaaaga agcactgcga ctgtacccac ctattcatat
     3841 tggtaaccgt cgtgtggcag aagaaatgga attcgcagaa ggtaaggtcc ctgctgggga
     3901 gcgtatgttc tactccattt acctcaccca tcgcgacccc gaggtctggg aaaatgctga
     3961 gcaattttgc cccgagcgtt tcgcccatgg acggaaaact ccgcccatga gttatattcc
     4021 gtttggcggt ggtccgcgtg cttgcattgg cgcagcattc ggtcaggcag aggcacggat
     4081 cgtcctctct cactt
//
LOCUS       DAOREF010000157         4094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13090_length_4094_cov_4.891310, whole genome shotgun sequence.
ACCESSION   DAOREF010000157 DAOREF010000000
VERSION     DAOREF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13090_length_4094_cov_4.891310"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKI33_04445"
     CDS             <1..336
                     /locus_tag="PKI33_04445"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNM36285.1"
                     /translation="LVFFWATWCPYCNVEISAVQMVHEAYSDDGLVVLAVNVGEGADA
                     ARQYGAAKNLTFSILNDAGRDVAADYAVTGYPTHYFIDASGVISSIDIGGMDYWSLVN
                     KVKPLLGLP"
     gene            517..795
                     /locus_tag="PKI33_04450"
     CDS             517..795
                     /locus_tag="PKI33_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36286.1"
                     /translation="MSKLSPRMLLFAALVLILVAGAFTTYRAVSANSERNSMGMYVLS
                     GGLVNQLKPQQPEGGTTDQNQVAPFPGSSGEGGHGGCESENNINPSDL"
     gene            887..2785
                     /gene="dnaG"
                     /locus_tag="PKI33_04455"
     CDS             887..2785
                     /gene="dnaG"
                     /locus_tag="PKI33_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNM36287.1"
                     /translation="MSTIDEIKSKIDIVDLVSEAGVKLRKAGRNMTGFCPFHDNKHTP
                     AFVIWPESGTWRCFGACNEGGDIFKFVMKREGIDFKEALNKLALRAGVEVQQIQRETP
                     QQREAYDHLRTLLEDAVVYYRSHLFANPEILNYLRQKRGLTDATIETFGLGYAPPGYD
                     NLLKHFSGKYMPQDLVDAGMLSERDSGGLFDRFRHRIMIPIRDENGKMAGFGARIVDP
                     NDNPKFLNSPETAIFTKGRLLYGFDRARKPIRAADQAVIVEGYLDVIALHQAGYENVV
                     SPMGTALTEDQLRLLKRSTRRIVLALDPDVAGQKAVLRGLEAARSAMDKEGELTFDAH
                     GLLRNEARLQADLRVATMPDDLDPDEIVARNKEEWATLIANAKPIITHVMEALSAGQN
                     LNDPKIKNQIAEQVLPLIDDMGTPMERDTYRQALARMLRVDERTLMAAQPKGAVMRRK
                     PRVTEPSRRTESVAVAAVNPRKNIEFYCLSVLIRKPDLVYRLDRKLEEFGLSPLATED
                     FEYTDHQLLFNVLRQAMGQDEKDHAQYVFSQIPEDLAPLVNELLAQTEKLESSDDKLL
                     EDLLARFLDLRRFHAMSSVTQLKFMQDDEQQQGGENIKVYIEQTMRFTRLLNGLDQAK
                     LKLSKRQA"
     gene            2956..3122
                     /locus_tag="PKI33_04460"
                     /pseudo
     CDS             2956..3122
                     /locus_tag="PKI33_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            3619..3960
                     /locus_tag="PKI33_04465"
                     /pseudo
     CDS             3619..3960
                     /locus_tag="PKI33_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1007 a          955 c         1086 g         1046 t
ORIGIN      
        1 ctggtgttct tttgggcaac ctggtgccct tactgtaacg tagagatctc tgctgtgcag
       61 atggtgcacg aagcttattc agatgatggg ttggtggttt tggctgtgaa tgttggcgaa
      121 ggcgccgatg cggcacgtca gtatggggca gcgaagaacc tgactttctc catcttgaac
      181 gacgccggaa gagatgtcgc cgcagactac gccgtgacgg ggtatcccac tcattacttt
      241 attgacgctt caggcgtgat ctcatcgatc gatattggcg gtatggatta ttggtctttg
      301 gtcaataagg tcaagccatt gctggggctg ccttagtttc gagattttgc ggggagggga
      361 aaagactcat tgtgttgaat tagagcgcaa tgagtctcct cttttaattg gataaaattg
      421 ggtataattt ttagtgtcca ttacggggga agtgaatgtt tccaactttg tacatcgatc
      481 ggatcgtttt gtatttacac gtaaggagaa aatcgtatgt ctaagttatc ccctagaatg
      541 cttttattcg cagccttggt tttgattctc gtcgctggag cgtttaccac gtatagagcg
      601 gtttcagcaa actctgagcg caatagcatg ggcatgtatg tgctcagcgg tgggctggtc
      661 aaccaattga aaccgcaaca acccgaaggt ggaacgacag accagaatca ggtcgcccct
      721 ttccccggca gcagcggaga gggtggtcat ggtgggtgtg agtctgagaa caatatcaac
      781 cccagtgact tgtaaaatgg attttgtaaa aataaggcag cgaaatggct gccttatttt
      841 atttatcgtt tgcacatcct accattcacg cctataattc acgtgcatgt ccaccattga
      901 tgagatcaag tccaagatag atatcgttga cctcgtatct gaagcggggg tgaagttacg
      961 aaaagccggt cgcaacatga ccggtttttg tccgtttcac gataacaaac atacgcctgc
     1021 gtttgtgatc tggcccgaat caggcacatg gcgctgtttt ggggcatgca atgaaggcgg
     1081 ggatatcttc aagttcgtga tgaagcgtga aggcatcgac tttaaagaag ccttgaataa
     1141 acttgcgctt cgagccggtg tggaagttca acagatccaa cgcgagacgc cgcaacaacg
     1201 cgaagcgtac gatcatttgc ggactctgtt ggaagatgcc gttgtttatt atcgcagtca
     1261 tttatttgca aaccctgaga tattgaatta tctgcgccaa aaacgcgggt tgacggacgc
     1321 taccattgag acctttggtt tgggttatgc ccctccaggc tatgataatc tgctcaaaca
     1381 tttttcgggc aagtatatgc cgcaggatct tgtagatgca ggcatgctct ctgaaagaga
     1441 tagcggcgga ttgtttgatc gttttcgtca tcgcattatg attcccatcc gcgatgagaa
     1501 cggcaagatg gcgggttttg gcgcacgcat cgtagacccg aacgataacc ccaaattttt
     1561 gaattcacct gaaacagcga tctttaccaa ggggcgtttg ttgtatggct ttgaccgcgc
     1621 tcgtaagccc atccgcgcgg cagaccaagc tgtgatcgtg gaagggtacc tcgatgtgat
     1681 cgctttgcat caggcgggat atgagaatgt ggtctcgccg atgggcacag cgctgactga
     1741 agaccagttg cgcttgctca agcgttctac ccgcagaatt gtcctcgcgc tcgacccaga
     1801 tgtggcaggt cagaaagctg tgttgcgcgg gcttgaagcg gctcgctctg ccatggataa
     1861 agaaggggag ctgacctttg atgcacatgg cttgctccgc aacgaagccc gcctccaagc
     1921 ggacttgcgt gtagcgacca tgcccgatga tctcgacccc gatgagattg tggcacgcaa
     1981 caaagaagaa tgggctacat tgattgcaaa tgccaagccc atcatcacac atgtgatgga
     2041 agccctttct gccgggcaga atttgaacga ccccaagatt aagaatcaga tcgccgaaca
     2101 agtgttaccg ctgatcgatg atatgggtac gcccatggaa cgcgatacgt atcggcaggc
     2161 gttggcgaga atgttacggg ttgacgaacg caccttgatg gctgcccagc ccaaaggcgc
     2221 ggtgatgagg cgcaagccac gtgtcacgga acccagccgc agaacagagt ccgttgcggt
     2281 tgcggcagtg aatccaagaa agaatattga gttctattgt ttgagcgttt tgattcgcaa
     2341 gccggatctg gtctatcgtt tagaccgtaa gttggaagag tttggcttga gcccattggc
     2401 aacagaagat ttcgagtata ctgaccatca gttacttttc aatgtattga ggcaggcgat
     2461 gggacaggac gaaaaagatc atgctcagta tgtgttctcg caaattcctg aagatcttgc
     2521 tccccttgtc aatgaattgc ttgcacaaac agaaaaactt gaatcatcag acgataaatt
     2581 gttggaagat ctcttagcac gctttttaga cctgcgacgt ttccatgcca tgagcagcgt
     2641 cacgcagttg aagttcatgc aagatgatga acaacaacaa ggcggagaga acatcaaggt
     2701 ctatattgaa caaacaatgc gctttacccg tttactaaac ggtttggatc aggcgaagtt
     2761 aaaattgtcg aaacgacagg cgtaattcaa cccgctttgt aggagacagg ttccatgaaa
     2821 ccgaaacgaa cgtctgccag caaaacaacg aagaaaaagg gcatggaaaa agaaaagaaa
     2881 aaggcagtga agcaaacatc gcccgaaaag ccccgcaagg gtcttcgttc gggtggctcc
     2941 ataggggatg aatccatgcc agagtttgtc acggatgatt ttgccgagcc cgatgacacg
     3001 ctctttgtgc cacaagaaga agaacttgat gaatctataa atatacatgc gcatttaact
     3061 ggcgagccgg gcgaagaccc agtgcgtttg tatttgcgcg agatcggtca ggtcaaatta
     3121 ttggcggcag atagcgagtt tcgccttgcc actctgatcg aaggcgaccg ccttatttcc
     3181 actttgaata aactcaaaca acgcaaaggg gtttcgcagg cggcgtccac ttatcattcc
     3241 gtgctttctg aagcgcttac ttcttgggat cgattggtgg aggataccga gcgccttgaa
     3301 catgacttgc ccaacttggc gctcttgctg gcagaggctc aatcgcttca tgccggatgg
     3361 gaatttgact ctccttccta tctgcgtgcc tacctcgaca acggtcagtg ggggagtgac
     3421 cacctatggg ataacctggc gcgtcatgct tatagtgttt tcctaagcct gtatctacta
     3481 ccatatccct atgcggaatg gttgttgtcg catgtgcgtg tgcatcaagg ctttcccgct
     3541 cagcggacct tgttccgtaa tttgccatcc gatgaagagt tgatttctga aatggaatcc
     3601 gtccatttgc gcgccatcga tgctcaacag gcattggtcc gcgctaattt gcgtttggta
     3661 gtgagcgttg ccaaacgtta tctggggcgt ggcattccgt tattagactt gatccaagaa
     3721 ggcaatatgg ggcttctgcg tgccattggc aagtttgacc cacggcgcgg attcaaattc
     3781 agcacctatg ccacttggtg gattcgtcag tccattaacc gttccatcgc cgaacaagcg
     3841 cgcaccattc gcattcccgt gcatctcttt gagtctattt cgcgcatctt gcgcgcccaa
     3901 cgtcacctca cgcaggtttt gggtcgtgaa cccacgaacg aagatatcgc tttggaagtt
     3961 ggctatctct ctgctgtgga tgtgcaatcc attttgcggg cgcatgccga gagcaagcct
     4021 gtccacccgg aattgcaacg caagttagat gtcgcttcgc aaaaggtcac tcgtgctttg
     4081 cgctctgccg aaga
//
LOCUS       DAOREF010000158         4061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13263_length_4061_cov_4.867449, whole genome shotgun sequence.
ACCESSION   DAOREF010000158 DAOREF010000000
VERSION     DAOREF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13263_length_4061_cov_4.867449"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..939)
                     /locus_tag="PKI33_04470"
     CDS             complement(82..939)
                     /locus_tag="PKI33_04470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNM36288.1"
                     /translation="MNESKSIHERKHVWRDEFLLVIGLCVFVGIVYALDNALHPVFTP
                     TTLLLTGVFLAIVPAFIWMIFFYLQDSAEPEPKGYVIGVFVLGGLLAASIGIPVVENL
                     FRVSHWIYTNTVATILGGILVVGFTQEFLKYAAVRYSIYHSDEYDEPTDGVIYATAAG
                     LGYATMLNINFVISNGGVDLGTGIIRMAVVALAHAAFSGITGYFLGRAKFESEQIWWM
                     PLGITLAATFNGLFNWLQGIVTRPTITLSGSTNNPWLGLALAAVVALITTGLVLTLVR
                     RNIKSLQAE"
     gene            complement(1058..1624)
                     /locus_tag="PKI33_04475"
     CDS             complement(1058..1624)
                     /locus_tag="PKI33_04475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNM36289.1"
                     /translation="MFPFVKLTDDVVMIRPYEFGDEHELHAAIQESLPELNPWMSWAT
                     ERYTLNVARDFVAITRAEWSRGTLYSFAILNASTNEFLGGCGLSHFHPVYKFCNLGYW
                     VRTSKHGNGYAGCATKLVAKFAFERANAIRAEIVIAKDNHASKRVAEKVGAHYEGILL
                     NRMTVRENIYDAHMFSLLPSDFGLVAQL"
     gene            complement(1709..2365)
                     /locus_tag="PKI33_04480"
     CDS             complement(1709..2365)
                     /locus_tag="PKI33_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36290.1"
                     /translation="MKKQRIILVDDHEVVRLGLKSLLERHPHFDVVGEAGSAREALEQ
                     TALLKPDVVVMDIRLPGTSGIEACEQIVAQFPNTKVIMLTSYAEDEMLFSAIRAGASG
                     YILKQIGSEDLIKALESVGRGEALLDPAVTQLVFQEVRRAVKEEEASAFAHLSQQEKH
                     VLLLVSEGKTNREIAKNLFLGEGTVRNYVSSILSKLNVNNRAEAAAYAVEHNLRDYIS
                     "
     gene            complement(2411..2881)
                     /locus_tag="PKI33_04485"
     CDS             complement(2411..2881)
                     /locus_tag="PKI33_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM36291.1"
                     /translation="MKELFEYREKFLARLREVTVEFCDACQAFKDPFTAVDGEWTVHQ
                     IAFHVRGIEREVYGTRVRRTLQEDNPEFKNFDPETWMADSYNRDENLETILDEFSKRM
                     NEVCDLLGDMPQEGWSRLSRHEALGKGLTLQLWAERGLAHIEEHLKALKKAQTL"
     gene            complement(2878..3465)
                     /locus_tag="PKI33_04490"
     CDS             complement(2878..3465)
                     /locus_tag="PKI33_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM36292.1"
                     /translation="MDPLKGKVVLITGAGKGIGRKLAETLAAQGAIVAANDISPINVD
                     EVVNGILAKGGQAKAYLEDVAKKVGAQMIVKSVEDEFGSLDILINHAAVEPHTPLLNM
                     DEWDWHRVLDVNLTGAFLMSQTAGRVMKEKGSGVIINLVAGAGERSKEAGAFLASKAG
                     LDELSRQLARELNPHGVKVVSASTLEEAIKALEEV"
     gene            complement(3472..>4061)
                     /locus_tag="PKI33_04495"
     CDS             complement(3472..>4061)
                     /locus_tag="PKI33_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36293.1"
                     /translation="DWFQDQSSRPELMMTSGSKCGDAPFIFPTNGLIGFIWDDSFRPG
                     HRHSGLDIFSGTEVGVTPIVAAYPGYLTREADWISTVIIRVPEDPLKPNRQIWVYYTH
                     MADPNGNSFVSSAFPQGTKEVFVEAGTFLGYMGNYSGDPANPVGIHLHISVVKDDGFG
                     NYTNELDINNTYDPSPYFGMPLNANENTDVIPVCN"
BASE COUNT         1063 a         1035 c          949 g         1014 t
ORIGIN      
        1 gcgatcaggg ttacaagaac aacggtgatc tccgcaaagc ggtcaccgag agaaccttgt
       61 ttgtttgcca tgtctgttct cctactcagc ctgcagcgat ttaatgttgc ggcgcaccaa
      121 ggtgagcaca agcccggtgg tgatcaacgc aacaaccgcc gcaagtgcca aacccaacca
      181 gggattattg gtcgagccgc tcaaagtgat ggtcggtctg gtgacgatac cttgcaacca
      241 attgaataag ccgttgaaag tggcagccag agtgatgccg agaggcatcc accaaatttg
      301 ttcagattcg aacttggcac gcccgaggaa gtagcccgta atgcccgaga atgccgcatg
      361 cgcaagggca acaactgcca tacgaatgat gcctgtgccc aagtccacgc cgccattgga
      421 gatgacaaag ttgatgttga gcatggtcgc atagcccaac ccggcagctg tggcatagat
      481 tacaccgtca gtcggttcat cgtattcgtc ggaatgatag atgctatacc gaacggcagc
      541 atactttaga aactcttgtg tgaagcccac tacaagaatt ccgcctagaa tggtggcaac
      601 tgtattggta tagatccaat gagaaacgcg gaagaggttt tctacaacag gaataccgat
      661 ggaagccgct aaaagtccgc ccaatacgaa gacaccgatc acatagcctt tcggttcagg
      721 ttcggcgcta tcctgcagat agaagaaaat catccaaata aatgcaggga caatggcaag
      781 aaagacacct gtaagcagca aggtggtggg ggtaaacacg gggtgtaggg cattatctag
      841 ggcgtagacg atacccacaa aaacacataa gccgataacg agcaggaact cgtcacgcca
      901 tacatgcttc cgttcatgaa ttgatttgga ttcattcatg gaattctctc tccttagtaa
      961 gtgatccatc aaataacaac cccatcatag taaaaagatg ggtgtacgtc aatagagctg
     1021 ggttacaaat gggggacaga ctgagcggag aaaatgctta cagttgggca accagcccaa
     1081 agtctgaggg gagaagcgag aacatatgcg catcataaat attctcgcgg acggtcatgc
     1141 gattcagcaa aatcccttcg taatgcgctc ccactttttc tgctacacgt ttgctggcgt
     1201 ggttatcctt tgcaattacg atctctgcac gaatcgcatt ggcgcgttcg aaggcaaact
     1261 ttgctacaag ttttgtggca caaccggcgt atccattgcc atgcttggag gtccgcaccc
     1321 aatagccgag gttacaaaac ttatagaccg ggtgaaaatg gcttaagcca caaccgccaa
     1381 gaaattcgtt tgtgcttgca ttcaggatgg caaaagaata taaggtgccg cgtgaccatt
     1441 ctgcacgcgt gatggcgaca aaatcgcgcg cgacattgag cgtgtatcgt tcggttgccc
     1501 aactcatcca cggatttaac tccggtagcg attcttgaat cgcagcatgc agttcgtgtt
     1561 catccccgaa ctcatatggg cgtatcatca caacatcatc ggtcaatttt acaaaaggga
     1621 acatagtttt ttaggcggga ataaaaaaag agacacttct tcacgcgggg tgcgaaagtg
     1681 tctctttcat agtggctgag aaaacgaatt aggaaatgta atctctcaaa ttgtgttcca
     1741 ccgcgtaggc tgctgcttca gcgcggttgt tcacatttaa cttcgagagt atgctcgaaa
     1801 cataattgcg gaccgttccc tcaccgagga agaggttctt ggcgatctcg cgattggtct
     1861 tgccttcaga gacgagtaaa agcacgtgtt tttcttgctg gcttaagtgt gcaaaggctg
     1921 aagcttcttc ttccttcacg gcgcggcgca cttcttggaa gacgagctgg gtcacagcag
     1981 ggtctagtag cgcttcacca cgtcccactg attcgagggc tttgatcaga tcttcgctgc
     2041 caatttgttt gagaatgtag cccgaagcac ccgcacgaat ggcagaaaag agcatttcat
     2101 cttctgcata cgaagtgagc atgatgactt ttgtgttggg aaattgcgca acaatctgtt
     2161 cgcaagcttc aatgccagag gttcccggca ggcgaatgtc catcaccacc acatcaggtt
     2221 tgaggagagc cgtctgttcc aacgcttcac gcgccgaccc agcttcaccc acaacatcaa
     2281 aatgggggtg tctctctaaa agcgacttca gtccgagccg cacaacttca tgatcgtcaa
     2341 ccaagatgat tctttgtttc ttcataatag gcactatttt accgcaggaa attggaaaaa
     2401 cgggcaaaaa ttatagggtt tgtgcttttt tcaaagcttt caaatgttct tcaatgtgtg
     2461 ccaacccgcg ttctgcccac aactgcaaag ttagaccctt gcccaaggct tcgtgtcggc
     2521 tcaaacgtga ccaaccttct tgtggcatgt cacccaatag atcgcagact tcattcatgc
     2581 gcttcgaaaa ttcatccaag atagtttcaa ggttctcgtc acgattataa gaatccgcca
     2641 tccatgtttc agggtcaaaa tttttgaact cgggattgtc ttcttgcaat gtgcggcgga
     2701 cgcgtgtgcc atatacctca cgttcaatgc cacgcacatg aaaagcgatc tgatgcaccg
     2761 tccattcgcc atcaacggct gtaaatgggt ctttgaatgc ctggcatgca tcacaaaact
     2821 caaccgtcac ttcgcgcagg cgtgctaaaa atttctcgcg atattcgaac agttctttca
     2881 tacttcctcc aatgctttga tagcttcttc caaagtcgaa gcagagacca ccttcacacc
     2941 atgcggattt aattctcgtg ccaactggcg ggacaattca tccaagccgg ctttgctggc
     3001 aagaaacgct cccgcctctt tcgacctttc gcctgctcct gccaccaagt tgataatcac
     3061 gccggagcct ttttctttca tcacccgccc cgccgtttga gacattagaa aagcgcccgt
     3121 tagatttaca tccaacacgc ggtgccaatc ccattcatcc atattgagca aaggggtatg
     3181 cggttccacc gctgcatggt tgatcaatat atcaagcgac ccaaattcat cttccacgct
     3241 tttgacgatc atttgcgcac cgaccttttt ggcaacatct tccaaataag cctttgcctg
     3301 ccctcccttt gcgaggattc cgttcaccac ctcgtccaca ttaattgggg agatgtcatt
     3361 ggctgccaca attgcacctt gagccgccaa agtttctgcc aatttgcgac caattccctt
     3421 gcctgctccg gtaattaaaa ccactttgcc tttcaatgga tccatccttt cttaattaca
     3481 cactggtatt acatctgtgt tttcatttgc attcaacggc atcccaaaat aggggcttgg
     3541 gtcgtaggtg ttgttaatgt ccaactcgtt tgtgtaattc ccgaagccat catctttaac
     3601 caccgaaata tgcaagtgga ttcccactgg gtttgccggg tcaccggaat aattacccat
     3661 atacccaagg aatgtccctg cttcaacaaa gacttctttg gtgccttgtg gaaaggcaga
     3721 agagacaaaa gaatttccat ttgggtctgc catatgggtg taatacaccc atatctggcg
     3781 attgggcttc aacgggtctt ctggcacacg gataattacc gtagaaatcc aatctgcttc
     3841 gcgagtgaga taacctgggt aggcagccac aatgggtgtt acgccaacct ccgtcccact
     3901 gaaaatgtct aacccgctgt ggcggtgacc ggggcggaat gaatcgtccc aaatgaagcc
     3961 gatcaagcca ttggtgggga agatgaatgg agcatcgccg catttcgagc cgctggtcat
     4021 catcagctca gggcgagagg actggtcttg gaaccagtca a
//
LOCUS       DAOREF010000159         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13349_length_4040_cov_4.733501, whole genome shotgun sequence.
ACCESSION   DAOREF010000159 DAOREF010000000
VERSION     DAOREF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13349_length_4040_cov_4.733501"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKI33_04500"
     CDS             complement(<1..380)
                     /locus_tag="PKI33_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36294.1"
                     /translation="MSTEKLHIDPYERNWMIISAILLVLFATAVSVAAFGLGIQVPAP
                     EQRVDPKTVATDPNSPWSNPGLREIAPGKYDAYVLARAVPNWEYLPKEMSIPAGSSVS
                     FYVTSADLQHGFKIQNTNANFMVIP"
     gene            complement(377..514)
                     /locus_tag="PKI33_04505"
     CDS             complement(377..514)
                     /locus_tag="PKI33_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HNM36295.1"
                     /translation="MAKKDKEDDEFRPTGTIAVLTIFVITLIAIWATIYMILVGRGGT
                     L"
     gene            complement(628..1215)
                     /locus_tag="PKI33_04510"
     CDS             complement(628..1215)
                     /locus_tag="PKI33_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNM36296.1"
                     /translation="MKIRFFLVGIATILVLIAGIYFLRPHTFHGTVIQSPDPSFDFTL
                     KSADGDVSISDFRGKLVLLYFGYTFCPDICPGTLANVAQALREMGTKADDVQLIMVSL
                     DPERDTPEKLKEYMGHFYPSFVGVTGSKEQLDEITSLYGVFYQKKEGSVATGYLIDHT
                     ATLMVLDREGYLKLVFPFGVTSEEIADDLKYMLRQ"
     gene            complement(1231..>1910)
                     /locus_tag="PKI33_04515"
     CDS             complement(1231..>1910)
                     /locus_tag="PKI33_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNM36297.1"
                     /translation="CMNKYALNGLYTLFGALAIAAFAFKVFQPIQVLPRMRLSPAYSF
                     INQDGETVTSEDLRGQFVLYSFTYTNCPAPCYNMGETMKEVQARLNEVDLGGIPVSFV
                     TISIDPDRDTPEALKTYGTDLGANFDDWMFVTTTNKALLKTIVGSGFETYYEDKGNGS
                     FALDPGYVLVDGWGIIRAEYRYSTEVSDADRIIRHMGVLADEVKNSKGSTKLAYEAAH
                     LFLCYAP"
     assembly_gap    1911..2010
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2057..2563)
                     /locus_tag="PKI33_04520"
     CDS             complement(2057..2563)
                     /locus_tag="PKI33_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNM36298.1"
                     /translation="MFRVNRQTDYAVRVVLALAKKPQGTRLPTAEIGREMLIPPSLLQ
                     RIVAELANGGYIKTQPGRDGGISLAHLPSEITLLQVVEHFEGNLVISDCILKEGDCPF
                     ETKCPVSCQWKRLNDLLIAEMSRITFEQLVEDGKQIEASQMTTTASNQLKVVSNQKSF
                     SLTDSLSA"
     gene            2819..3865
                     /gene="lgt"
                     /locus_tag="PKI33_04525"
     CDS             2819..3865
                     /gene="lgt"
                     /locus_tag="PKI33_04525"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNM36299.1"
                     /translation="MIDPVIFSFKLFTWQVTLTWYGLIVMSGVLVAAWFAEREVRRRG
                     ENGEVLIDAMVWAVVAGIIGARLWYVMNALIGGNRSYIEDPMSIIRPPIAGLHFFGGL
                     LFGAIALIIYLRNNGYDVWLFMDAVAPVALLGQAVGRIGNYINQELYGPPTQLPWGIS
                     IPADHRLAQYADLAKYPVESTRFHPTFAYEMVLNVLAFLFIMWYARRDEEEPKPGTLF
                     SMWLIAAGFIRTFIEFFRPDQPRLGDTFITTSMLVAFLMGVTGVILFLARHGKLQIAA
                     LTDWEEEYFIKKVEKKSAPRARKVESAAVVETDSEPEVDEPVVKRKSPARAKATTGKK
                     PTTKKAPAKRKKTE"
BASE COUNT         1003 a          846 c         1003 g         1088 t
ORIGIN      
        1 gggatgacca tgaaattggc attcgtgttc tggatcttga acccatgctg aaggtcagca
       61 gaagttacat agaaagagac gctggaacca gcaggaatgc tcatttcctt gggtaaatat
      121 tcccagttgg gaactgcacg tgcaagcaca taagcatcat atttaccggg ggcaatctcg
      181 cgcaagccag ggttcgacca ggggctgttc gggtcggtgg caaccgtctt cgggtccacg
      241 cgttgttcag gtgcagggac ctgaatgccc aaaccaaagg ctgctacact cacggcagtt
      301 gcgaacaata cgagcaggat ggcgctaata atcatccaat tgcgttcgta tgggtcaata
      361 tgcaatttct ctgtgctcat agtgtgcctc cacgtccgac aaggatcata tagatcgttg
      421 cccagattgc gatgagtgtg atcacgaata tcgtcaagac tgcaatggtg ccagtcgggc
      481 gaaactcatc gtcttcttta tctttcttcg ccatgttaca ttctcctctt gaaaaaatga
      541 aattaggatg actaccgctt cacgcggcgc cgcaagctgc ggtggtggat aatgggcagt
      601 tctctacgct gtagcaggtc cacctcctta ttgccgtagc atgtacttca gatcatcggc
      661 aatctcttca gaagtgacgc cgaatggaaa taccaacttc aaatatcctt cccgatccaa
      721 taccattaaa gtagctgtat ggtcgatcaa atacccggta gcaacactgc cttccttctt
      781 ttgatagaag accccataca gtgaggtaat ttcgtctagt tgttctttgc tacctgtaac
      841 gcccacaaac gaaggataaa aatgccccat gtattccttt aacttttcag gggtatcccg
      901 ttctgggtca agcgagacca tgatgagctg aacatcatcg gcttttgttc ccatttctcg
      961 caaagcctga gccacatttg caagcgtgcc ggggcaaata tctgggcaga aggtgtaacc
     1021 gaaataaagc aataccagtt tgccgcggaa gtcgcttatg gaaacatcgc cgtctgcgct
     1081 tttaagggtg aaatcaaatg aagggtcagg ggattgaatg accgttccat gaaaggtgtg
     1141 agggcgtaaa aaatatattc ctgctatcaa aacaagaatt gtcgcaatac caacaagaaa
     1201 aaatcggatc ttcataataa cgatggacct ttatggcgca taacaaagga agaggtgagc
     1261 cgcttcatat gctagtttgg tagaaccttt gctgttctta acttcatctg ccagaacccc
     1321 catgtggcgg atgatacggt cagcgtctga gacttcagtg gagtagcgat attcagcacg
     1381 gatgatgccc cagccatcta caagaacgta accggggtcg agggcaaaac taccatttcc
     1441 tttgtcttcg taatacgttt caaaacctga gccaacaatg gtcttgagta aagccttgtt
     1501 tgtggtggtg acgaacatcc agtcgtcaaa gtttgcgccg aggtctgtgc cgtaggtttt
     1561 caacgcttcg ggcgtatctc ggtctgggtc gattgagatg gtgacaaatg aaaccggaat
     1621 accgcccaaa tccacttcgt tgaggcgggc ttgtacttct ttcatggttt cgcccatgtt
     1681 gtagcaaggt gctgggcagt tggtgtatgt gaaggagtag aggacgaact gtccgcgtag
     1741 gtcttcgctg gtaaccgttt cgccatcttg gttgataaaa ctataagcag gggagaggcg
     1801 catgcggggg agtacttgaa tgggctgaaa aactttaaat gcgaaagcag caattgcaag
     1861 agcaccaaag agtgtgtaca gaccgttgag agcgtatttg ttcatgcaga nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaagagcac caaagagtgt gtacagaccg
     2041 ttgagagcgt atttgttcat gcagaaaggc tgtcagtgag agaaaagctt ttttgattag
     2101 aaacaacttt gagttggttt gaggcggttg ttgtcatctg gctggcttcg atctgttttc
     2161 cgtcttctac caattgctcg aaggtgatac gagacatttc tgcgatcagt aggtcattta
     2221 agcgcttcca ctgacagctc accgggcatt tcgtctcaaa ggggcaatcg ccttctttta
     2281 atatgcagtc tgagatgacc aaattgcctt caaagtgctc tacgacctgt aacagggtga
     2341 tttcgctagg gaggtgagcc aagctaatac cgccgtcgcg cccaggctga gttttgatgt
     2401 agccgccgtt tgccaattca gccacaatgc gttggagcaa agaaggcgga atgagcattt
     2461 cgcgtccgat ttctgcagta ggcaaacgag tcccttgcgg ttttttcgca agagccagca
     2521 caacgcgaac ggcataatcg gtttggcggt ttactcggaa cattaatatc ctttggttta
     2581 acattgacat ttgatgtcaa tgtttactga gattgtaagg atttggggct ttcttttgac
     2641 gtgttattaa tcacattgtt aaatgacaaa cgtctcgtat tcttgtcagc acgtgaagta
     2701 tttatgacag aatttgttgt atttagcaaa aagtaacaat gtgccttata ttgggctata
     2761 tgtattgcat ggtttgattt ggggatataa tcgtcaaaac ttaactcaga ggtaatgaat
     2821 gatagacccc gttattttca gttttaaatt attcacgtgg caagtcacac tgacatggta
     2881 tggcttgatc gtgatgtctg gtgtgttggt tgccgcttgg tttgccgaac gtgaagtgcg
     2941 ccgccgtgga gagaatggtg aagtattgat cgacgccatg gtgtgggcgg ttgtagctgg
     3001 cattattggt gcgcgcttat ggtatgtcat gaatgcgctg attggtggca atcgttctta
     3061 tattgaagac cccatgtcca ttattcgccc gccaatcgct ggtttacatt tcttcggtgg
     3121 tttgcttttt ggtgcaattg ctctcatcat ttatctgcgc aacaacggat atgatgtgtg
     3181 gctgttcatg gacgcggttg cgcccgtggc attgcttggg caggctgtag gcagaattgg
     3241 taattacatt aatcaggaac tgtacggtcc gccaacacaa cttccatggg gtatttcgat
     3301 ccctgccgat catcgccttg ctcaatatgc agatttggcg aagtacccgg tagagtcaac
     3361 gcgctttcat ccgacctttg cttatgaaat ggtcttgaat gttttggctt ttcttttcat
     3421 catgtggtac gcccgtcgtg atgaggaaga gcccaagcct ggcactctct ttagcatgtg
     3481 gttgatcgct gctggattca ttcgtacgtt tattgaattt ttccgcccag atcaaccgcg
     3541 ccttggcgat acttttatta ccacctccat gttggttgcg ttcctcatgg gcgtaactgg
     3601 tgtgattttg ttcctcgctc gccatggcaa acttcaaatt gcagcattga cagattggga
     3661 agaagagtat ttcatcaaga aagtggaaaa gaagtctgcg cctcgcgctc gcaaagtaga
     3721 atctgcggct gttgtggaaa cagatagcga acccgaagtt gatgaacccg ttgtaaaacg
     3781 aaaatcccct gctcgggcga aggctacaac agggaagaaa ccgactacca agaaagcacc
     3841 tgccaaacgt aagaagacag agtaaaacct gatttacaaa caaaaaagct gatgataccc
     3901 atcagctttt tgtttgtaaa agtaagtaag ggcggtgtat agttgcttat cgaacataat
     3961 aagttctgct agttggagca taacaaatga aaattgcagt atttggcact ggcggtgtgg
     4021 gtggatattt cggcgggcgg
//
LOCUS       DAOREF010000160         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13355_length_4040_cov_3.276286, whole genome shotgun sequence.
ACCESSION   DAOREF010000160 DAOREF010000000
VERSION     DAOREF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13355_length_4040_cov_3.276286"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKI33_04530"
     CDS             <1..385
                     /locus_tag="PKI33_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36300.1"
                     /translation="TQEVSGKVTITAKSDQKVLSVRVAVVEISNEEFNGQQREMKKDL
                     AFAKLDGFELKTDETRTLPFTIALVAGDKVSVNIFGGTLEISGGPSLSSSNSIFSPVK
                     YQIVAVADCEGVAIDPTDAKPLTIL"
     gene            644..1414
                     /locus_tag="PKI33_04535"
     CDS             644..1414
                     /locus_tag="PKI33_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36301.1"
                     /translation="MKYVLDVNMCRNNQLVNLLKEKNEILITSDFLVEVFKANNPKFM
                     FVNNTRIIKEYPSQIFVTHDRGELVRLEISKKSPLIPDEIIDLNSTNNFRNWLINENE
                     IDLILQLVYLEAKRQVEYQKDFMNTYMYQATKKLQGLLKFENTRKQYLTDNAKLLNDI
                     KEVSITVMEGFLEKKNIINVENFKRVNSVIYSQNYVLLWRIAKWSLLNGIESVSLDKL
                     THDGFDIKYIFISSFFDGLLTKETWLKECRFDLINSIG"
     gene            1451..1837
                     /locus_tag="PKI33_04540"
     CDS             1451..1837
                     /locus_tag="PKI33_04540"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNM36302.1"
                     /translation="MPTNPIDLFISNYPPEVQTIMQKIRATIHKSAPAAEETIGYGIP
                     TFKLNGKNLVHFSAFKEHIGFYPTPTGIEHFKKELSAYEGAKGSVKFPLNKPIPYALI
                     TKIVQFRVKEVSAQTAPKKKITKTKV"
     gene            1834..2361
                     /locus_tag="PKI33_04545"
     CDS             1834..2361
                     /locus_tag="PKI33_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNM36303.1"
                     /translation="MNTFFTSDTHFGHQRTLEFSRRPFADVSSMDAALIANWNAVVQP
                     GDTVYHLGDFGAQECLDYVGKLNGKMIFLPGNYETPEMIRVLAQSCEIIQPNTIIEVE
                     GHRFQLVHEPDEATAGEFFLFGHVHQLQMVKRNGLNVGTDCHFFRPIPLDVVLFYQNA
                     IQKHYDDNVFMERLG"
     gene            complement(2398..3528)
                     /locus_tag="PKI33_04550"
     CDS             complement(2398..3528)
                     /locus_tag="PKI33_04550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNM36304.1"
                     /translation="MENRIKELDGLRGIAVVLVMALHLFKRAAYFTEHPVLLGFTKLT
                     TVGWVGVDIFFTLSGFLITSILLKSKTDEHYFKNFYVRRALRIFPLYYAAIAFVLFFA
                     PKVEEEFTNQLSTALPIMLLYQQNWALLFKDFHITQYLGITWSLAIEEQFYFLWPFIV
                     YKLDRNTLIKVSIGYIAVSILGRVLGTLLWPNLGESSTFFYYTSFARFEEMLFGGLLA
                     TFLTYEGALEKVKRYALPLFLASFTVFVALHILSLPGDPHPEHASLPLTLGGYTTAAL
                     FTLGLIGIFVTRPPQNLFRRFFANPVLTFLGKYSYSMYLFHMTAALILLDVFWHSELR
                     GWKPYFLYPLSVYVVTVIIALLTWNLLEKHMLGLKKYFEYKS"
     gene            complement(3530..>4040)
                     /locus_tag="PKI33_04555"
     CDS             complement(3530..>4040)
                     /locus_tag="PKI33_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNM36305.1"
                     /translation="NSIRILIGVETAKGILNLKEIAAHPRLDAIIFGGEDFAASIGAT
                     RTKDAIELLYARQAVIVACAAYDLQPIDIVTIDYKDLEALKVESEFGARLGFSGKQVI
                     HPNQVPVVQEAFTPSAEAITYARRIVETFEASQKEGKGAYSLDGKMIDMPLLKNAQKV
                     LARAKAAGK"
BASE COUNT         1266 a          801 c          868 g         1105 t
ORIGIN      
        1 cacgcaagaa gtttcgggaa aagtgaccat cacagccaag agcgaccaga aggttttgtc
       61 tgttcgtgtg gcggtggtcg aaatatcaaa tgaagaattt aacgggcaac agcgcgagat
      121 gaagaaagac cttgcatttg caaagctgga tggatttgag ctaaaaacag atgaaacccg
      181 cacgctgcct tttacgatcg ctttggtggc aggagacaag gtatctgtga acatcttcgg
      241 tggcaccttg gaaatttcag gcggaccgag cttatccagt tccaattcca tcttctcgcc
      301 ggtgaagtat cagattgtag ccgttgcaga ttgtgaaggc gttgctattg accccaccga
      361 tgccaagcct ctaacgattt tgtagtagtt tcatttttat ctttagaaaa atcccttcta
      421 catcatagaa gggattttta ttttgaggat gtgttgtggt ttacaataat aaaaaaggtg
      481 aataatagaa gaagtatatt tccaaccttt cagataatgt tacctaacta tctgaaagaa
      541 atttgaggcg ttcttcaaaa gatttgggca atgatttgaa ctaaaaatct aatttaagtg
      601 tgaattattt tttgaagaat acctaatgcc ggagatatgg aaaatgaaat atgtgcttga
      661 tgttaatatg tgcaggaata atcagctagt aaatcttctt aaagaaaaaa atgaaatatt
      721 aattacaagc gattttctag ttgaggtttt taaagctaat aatcctaaat tcatgtttgt
      781 gaacaatact aggattataa aagagtatcc aagtcagata tttgttacac atgatagagg
      841 agaacttgta agattggaaa taagcaagaa gtcccctcta atccctgatg aaattattga
      901 tttgaactct acaaataact ttagaaactg gttaattaat gaaaatgaaa ttgatttgat
      961 attacaactg gtttatcttg aagcaaaaag acaggtcgag tatcaaaaag actttatgaa
     1021 tacatacatg tatcaagcta ctaaaaaact tcaaggctta ttgaagtttg aaaacactcg
     1081 aaaacaatat cttaccgata atgcaaaatt attaaatgat attaaggaag tatcaatcac
     1141 tgttatggaa ggatttcttg aaaagaagaa tatcataaat gttgaaaatt ttaagagagt
     1201 aaattctgta atttactcac aaaattatgt tttgctttgg agaatagcaa aatggtcatt
     1261 gctgaatgga atagaatctg ttagtttaga taagcttaca catgatggct ttgatattaa
     1321 gtatattttc atatcttctt tttttgatgg tttattgact aaagagactt ggttaaaaga
     1381 gtgtcggttt gatcttatta acagtattgg ataattaata gaagaaatta acacggaaag
     1441 gaaactgtaa atgcctacca accccattga cctatttatt tcaaactatc cacccgaagt
     1501 ccaaaccatc atgcaaaaga ttcgtgcaac gatccacaaa tctgcgccag cggcagaaga
     1561 gacgattggc tatggaattc ctaccttcaa gttgaatggt aagaatcttg tgcacttctc
     1621 agctttcaaa gaacatattg gcttttaccc cacccctaca gggattgagc attttaagaa
     1681 agaactctct gcgtatgaag gggcgaaggg ttcggtcaag ttcccgctga acaaacccat
     1741 cccatatgct ttgatcacga agattgttca gttccgtgta aaagaagtct ctgcacagac
     1801 agcgcctaaa aagaagatta ctaagaccaa ggtatgaata cattttttac gtctgacacc
     1861 catttcgggc atcaacgaac cttagagttt tcgcgccgtc cctttgcgga tgtgtcctct
     1921 atggacgctg ccttgattgc caattggaat gccgttgtcc agccagggga tacggtctat
     1981 caccttggcg attttggggc gcaagaatgt ttggattatg ttggtaaact caatggaaag
     2041 atgattttcc tgccggggaa ttacgaaacg ccagagatga tccgagtcct tgcacagagt
     2101 tgcgagatca ttcagcccaa tacgattatc gaagtggaag gtcaccgatt tcaattggtc
     2161 cacgaaccgg atgaagcaac ggcaggcgag ttcttcctct tcgggcatgt ccatcaattg
     2221 cagatggtca aacgcaatgg attgaacgtt ggcacggatt gtcacttctt tcgccccatt
     2281 ccattagatg ttgttctgtt ctatcaaaat gccattcaaa agcattacga cgataatgtc
     2341 tttatggaaa gattaggata aaaaactccg aggttctcat gacctcggag ttttgcatta
     2401 agacttgtat tcaaaatact ttttcaaccc cagcatgtgt ttttcgagca aattccatgt
     2461 cagcagcgca atgatgaccg taacaacata taccgacagc ggatagagaa agtatggttt
     2521 ccagccgcgt agttcgctgt gccagaagac atccaacaag atcaatgctg cggtcatgtg
     2581 gaagagatac attgaataac tgtatttgcc gaggaaggtc agcaccggat tggcgaagaa
     2641 gcggcggaaa aggttctggg gtggacgagt aacgaagatg ccgatcaagc cgagggtaaa
     2701 aagagccgcg gtggtatatc cgccaagcgt gagaggcagg ctagcatgtt cagggtgagg
     2761 gtcaccgggt agagacaaaa tatgtagggc aacaaaaact gtaaatgaag ccagaaatag
     2821 cggcaaggca taccgtttga ctttttccaa agcaccttca tacgttaaga aggttgccaa
     2881 caaaccgccg aagagcattt cttcaaagcg tgcaaaagag gtgtaataaa agaatgttga
     2941 agattctcca aggtttggcc ataaaagcgt gcccagtaca cgcccaagaa tggaaacagc
     3001 aatgtatcca atgctgacct tgatcaaggt gtttcgatct agtttataaa caataaaagg
     3061 ccataagaaa taaaactgtt cttcaatggc aagcgaccaa gtaatcccca aatattgagt
     3121 gatatgaaaa tctttgaata acaatgccca attctgttga tacaacaaca tgattggcaa
     3181 tgctgtgctt aactgattgg tgaattcttc ttctactttg ggcgcaaaga aaagtacaaa
     3241 ggcaatggca gcataataaa gcgggaaaat tcgcaaggcg cggcgtacat agaaattctt
     3301 gaaatagtgt tcatccgttt tcgacttcaa cagaatggat gtgatcaaaa agccagagag
     3361 cgtgaagaaa atatcaacgc ctacccagcc gacggttgta agttttgtaa accctaatag
     3421 aacagggtgc tctgtgaagt atgcggcacg tttgaaaaga tgtaacgcca tcactaacac
     3481 tacagcaata ccgcgtaaac cgtccaactc tttgatccga ttttccatac tatttccctg
     3541 ccgcttttgc gcgtgccagc actttttgcg cattcttcaa taacggcata tcgatcatct
     3601 tgccatccag cgagtacgcg cctttgcctt ctttttgact ggcttcaaag gtctcgacga
     3661 ttctccgcgc atacgtgatc gcttccgcag atggagtgaa cgcctcttgc accaccggaa
     3721 cctgattcgg gtgaatgacc tgtttcccgc tgaagccgag tcttgctcca aattccgatt
     3781 caactttgag cgcttccaag tccttgtaat cgatggtgac aatatcaatg ggctgaagat
     3841 catacgccgc acaagcgaca atgaccgcct gccgcgcata caacaactca attgcatctt
     3901 ttgtccgcgt tgcaccgata gatgccgcaa agtcctcacc gccgaagata atcgcatcca
     3961 aacgtggatg tgccgcaatc tctttgagat tcaaaatccc tttcgctgtt tccacgccga
     4021 tcaaaatccg aattgagttt
//
LOCUS       DAOREF010000161         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13405_length_4031_cov_4.058853, whole genome shotgun sequence.
ACCESSION   DAOREF010000161 DAOREF010000000
VERSION     DAOREF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13405_length_4031_cov_4.058853"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PKI33_04560"
     CDS             <1..1192
                     /locus_tag="PKI33_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNM36306.1"
                     /translation="SVDSANLDDQVEVILPKTGFYIEAGGQVNDTGFIRGEGWEIEVT
                     GIRRASAGVIVHIGEVISGHPKVGDKATAEVDATRRHDIMRNHTATHLLHKALHEVLS
                     DQAKQAGSLVSPTHLRFDFNHPEAMTADQIERVEKMVNEAVAMDMPVVKVTKSLDEAK
                     KEGAMALFGEKYGEVVRTISIMEGEKDKYSYELCGGTHLERTSDVGAFIIVSESSAAA
                     GVRRIEAVTGRGAYELIQKRFKTLKQTAGSLKTSLDEVPTKVDALQDEVSELKKELAA
                     LRTQAALSTFNSLLANVQTAKDVNVLGAEIPNADVDTLRSLADKFREKYPKNGAAVFV
                     SGSTVIATVTEDVVKRGVKAGDLIAGIGGKGGGRPNLAQGSLSGDVAAALSRLGQVIE
                     AKVK"
     gene            1243..2292
                     /locus_tag="PKI33_04565"
     CDS             1243..2292
                     /locus_tag="PKI33_04565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNM36307.1"
                     /translation="MLNTSGVKRRQIYLTTFPVGALFVIVFIIVVREKLNFFSEVVGM
                     GSATMILLGSLVLYFQPLKVYTIETSFYFFMALFLPALAGALLTNPNTILNMSSALFA
                     ETINGLTLWVSIIFMGAFLSISPRAFKLLVILVFLVIFLVYVISAVALLQVYKWNFTY
                     LFHFLHSFFALGITMLLISRIGRIQQLHATTDKLTGLLNRYAGSAKLSEEFEYGAGNS
                     TDLAVILVDVDHFKEINDFHGHLVGDKVLKEFGDVISNFIRKTDSVVRWGGEEFLVIL
                     PNTTIENGARLAERLREEIDQRVFATQLHVTASFGVASRQHGQDMEALFHAADQAMYK
                     AKKGGRNQVVVYPIN"
     gene            2301..3302
                     /locus_tag="PKI33_04570"
     CDS             2301..3302
                     /locus_tag="PKI33_04570"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNM36308.1"
                     /translation="MNPFPAIQNAWYIACRSSQLKRKPITRTILNTPFVLFRNASGSA
                     AALIDRCSHRNVALSDGWVKDGCVVCPYHGWEFDGEGTCQNVPGLMYERPQKARNVSA
                     YPVVEQDGMIWVYPSLETPTSQPPRFEFLHEPGFTHIVAERTLPVALPDALENFLDAT
                     HTHFVHSGLIRSEGKRKAVNITVQREADRVQAEYIDEGTQSGLISRLFGAGIDKSFGR
                     YIFPSTAQLEYRAGENTKMLITLCFTPETETSQRLYAVVVGRGPAIFKPIIQLLFLQA
                     YQQDKHILDMQRENLKKFEKTQYVFTDADVLLPHILHIMKHGPTPVTASTRTVKLLL"
     gene            3312..3875
                     /locus_tag="PKI33_04575"
     CDS             3312..3875
                     /locus_tag="PKI33_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36309.1"
                     /translation="MSSPVTPRLNPFIYARIFSVPILWYFAWMDWQLALGIGICLSAL
                     TDILDGRLAAKDPAYSNPRLDSFADKLLTVSVLLWLILLKPFLFTDHAAWVGVAGLIF
                     ILSILISVVKFGRPTTLHLYSGKYGGLVQAFFIVHVFVSTVYVPLLFYIALGSFILAG
                     VEEILVLLLSDEIDEEKVKSILPFLNR"
BASE COUNT          992 a          911 c          973 g         1155 t
ORIGIN      
        1 gtccgtagac tccgccaact tggacgatca ggttgaggtc attctcccca agaccggttt
       61 ttatatcgaa gcgggtggtc aggtcaatga cactggcttc attcgtggag aaggctggga
      121 gatcgaagtc accggcatcc gccgtgcatc tgcgggcgtg atcgtgcata tcggtgaagt
      181 catctccggt catcccaaag tgggtgacaa agccacagct gaagtggacg cgacccgccg
      241 ccatgacatc atgcgcaacc ataccgccac gcacttgctg cacaaagcac ttcacgaagt
      301 gttgagtgac caagccaagc aggcaggttc acttgtctcg ccaactcatt tacgtttcga
      361 cttcaaccac cccgaagcca tgaccgccga ccagatcgaa cgcgtcgaga agatggtcaa
      421 cgaagcggtg gcaatggata tgcccgtggt caaggtgacc aagtcactgg acgaagccaa
      481 gaaggaaggt gcgatggcgc tcttcggcga gaaatacggt gaagttgtgc gtactatcag
      541 cattatggaa ggtgagaaag ataagtattc ctacgagttg tgcggtggaa ctcaccttga
      601 gcgcacgtcg gatgtgggcg cgttcatcat cgtcagcgaa agctcggcgg cagcgggtgt
      661 gcgtcgtatc gaagcggtca ccgggcgcgg cgcgtatgag cttattcaga aacgcttcaa
      721 gaccttgaag cagacggcag gctcgctcaa gacttcgctt gatgaagtcc ctaccaaagt
      781 ggacgcactg caagatgaag tctctgagct taagaaagaa cttgctgcct tgcgcactca
      841 ggctgcactt tctacattca actcactcct tgcgaatgtt caaaccgcca aagatgtcaa
      901 cgttctcggc gcggaaattc ccaatgcgga cgtggacact ctgcgttcac tggcagataa
      961 gttccgcgag aagtatccca agaatggtgc ggcagttttc gtttcgggca gcactgtcat
     1021 tgccaccgtg accgaagatg tggtcaaacg gggagtcaag gctggggact tgatcgcagg
     1081 catcggcggc aagggaggcg gtcgccccaa tctcgctcag ggcagtttga gcggtgatgt
     1141 cgctgcggca cttagtcgcc tcggacaggt tattgaagca aaggtcaagt aaatttaaag
     1201 ggtggttcgc cacccttttt gcttactagg attgatccgt ctatgttaaa tacgtctggc
     1261 gttaaacggc gacaaatcta tttaacaact tttccggtag gagcactctt tgttatagtg
     1321 tttattattg ttgtccgtga aaagttaaac ttcttcagcg aagtagtagg gatgggttcg
     1381 gcaacaatga ttttgctggg ttccttggtc ttgtactttc aaccactaaa agtgtataca
     1441 atcgaaacgt cattctattt tttcatggcg ttgtttttac ctgcacttgc gggggcgtta
     1501 ctaacaaatc caaatactat tttaaatatg tcatcggcat tatttgccga gactattaac
     1561 ggtttgaccc tttgggtatc cataattttt atgggtgctt ttctttcgat ttccccaagg
     1621 gcatttaaac tgttagtgat ccttgttttt ttagtcatat ttttggttta tgtgatctcg
     1681 gcagttgctt tgctgcaggt ttacaaatgg aattttacgt atttattcca ttttctacac
     1741 tctttctttg cattaggaat tacgatgctt ttgatctcgc ggatcggacg aatacaacag
     1801 ctccatgcaa caacggataa attaaccggg ttgcttaatc gatatgcagg ttcagccaaa
     1861 ctaagcgaag agttcgaata tggcgcagga aattcaacag atcttgcggt tattctggta
     1921 gatgttgatc atttcaagga aataaatgat tttcatggac atcttgtagg agacaaagtc
     1981 ctgaaagaat ttggcgatgt gatatccaat ttcattcgca agacagactc tgtagttcgt
     2041 tggggtggcg aagagttttt ggttattttg ccgaatacaa ccattgaaaa tggagcgagg
     2101 cttgctgaaa ggctgcgtga ggagatcgat caacgagttt ttgctacaca acttcatgta
     2161 actgccagct ttggtgtagc atcacgccaa catgggcagg atatggaagc cttgttccat
     2221 gctgctgacc aggcgatgta taaagcgaaa aaaggtgggc gaaatcaagt agttgtatac
     2281 cctataaatt gagcgtgctg atgaatccat ttcctgccat acaaaatgcc tggtatattg
     2341 cctgccgttc ctcccaactg aagcgtaagc ccattacccg cactatccta aacacaccct
     2401 ttgttttatt ccgcaacgcg agcggaagcg cagccgcatt aatagaccgc tgttcgcacc
     2461 gcaatgtcgc cttgtctgat ggctgggtaa aagatggttg cgtggtctgc ccgtatcacg
     2521 gctgggaatt tgatggagaa ggaacttgcc aaaacgtgcc cggcttgatg tatgagcgac
     2581 ctcaaaaggc tcgcaacgtt tctgcttacc cagtagtaga acaagatggg atgatttggg
     2641 tttatccctc actagaaaca cctacatctc agccgccgcg ttttgaattt ttacatgaac
     2701 ccggctttac acatattgtt gcagaacgta cattgccagt tgcacttcca gatgcactgg
     2761 aaaatttttt agatgccaca catacgcatt tcgtccactc tgggcttatt cgcagcgaag
     2821 ggaaacgcaa ggcggttaat atcactgtcc aacgtgaggc tgatcgtgtg caagcagaat
     2881 atatcgacga aggaacacaa tctgggttga tctcacgcct ctttggtgca ggaatcgata
     2941 aaagttttgg acgttatatt tttccttcta cggcgcaatt ggaataccgc gctggtgaaa
     3001 acactaaaat gctgattact ctttgtttca cccctgaaac cgaaacctca caaagattgt
     3061 atgcagttgt agttgggcgc ggaccagcta tttttaagcc tatcattcaa ctcttgttct
     3121 tgcaggcgta tcagcaagac aaacatattt tggatatgca acgcgaaaac ctgaaaaagt
     3181 ttgaaaaaac tcaatatgta tttaccgatg cggatgttct tttgccgcac attcttcata
     3241 tcatgaagca tggacctact cccgtcaccg cttccacccg gaccgtgaag ctgcttctgt
     3301 aattctgata gatgtcctct cctgtaactc cgcgcctcaa tccattcatt tatgcccgca
     3361 tcttttcggt gcccattctt tggtattttg catggatgga ttggcagctt gccttaggca
     3421 tcggcatttg cctctctgca ctgacagata ttttagatgg aaggctggct gccaaagacc
     3481 cggcttattc aaatccgcgc cttgattcgt ttgcagataa attgctgaca gtttcagtct
     3541 tactatggct gatcttgctc aaaccattct tgtttactga tcatgccgca tgggttggcg
     3601 ttgccgggct gattttcata ctgtctatac ttattagcgt ggttaaattt gggagaccca
     3661 caaccttgca tctgtactca ggaaaatatg gcggcttggt acaagctttt tttattgtgc
     3721 atgtgtttgt atctactgtc tatgtgccgc ttttgtttta tattgcgcta ggctctttta
     3781 ttctggctgg ggtggaagag attttagtac ttttgctatc agatgagatc gacgaagaaa
     3841 aagtgaaatc cattttgccg tttttgaatc gatgaagact ttttttagtg ccttacaaaa
     3901 acggtttgac ttgctcttgg tctccccaag attacctttc acattggctt tgttagcttc
     3961 tttgctgacc tttccatctc tttgggtggg gcttcaattt gatgactact tgttagagaa
     4021 atcctttcag g
//
LOCUS       DAOREF010000162         3988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13650_length_3988_cov_5.258581, whole genome shotgun sequence.
ACCESSION   DAOREF010000162 DAOREF010000000
VERSION     DAOREF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13650_length_3988_cov_5.258581"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKI33_04580"
     CDS             <1..695
                     /locus_tag="PKI33_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNM36310.1"
                     /translation="KSAKLLYVDWYDGDHIVRAMAEAKKHNVPVFLNFEHGHQNPELL
                     KKYAGMVTVCQAVTDAAQIGKRQAMLGTARKLIKSGIQTAIITMASQGCMVVQGDEII
                     RAYAPKVKAVDASGAGATFSSGYIYGYLNGWSLEDTVRFAIAAASLKVTRSGLDMFPV
                     SEIKGLARMVRIERMVYRGDQFRTIRKLLTLPENSPLVNNALVKESQKLAERILPKKK
                     IERKKIKRSLVE"
     gene            719..2155
                     /locus_tag="PKI33_04585"
     CDS             719..2155
                     /locus_tag="PKI33_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNM36311.1"
                     /translation="MSFAALLTQEQVEKIHDASLEILEDVGLKVRYEPARELFKKHGC
                     TVEDERVKFPRAVVEKYRKLVPPSFTFHARDPKFDKTIPQDSPVIVTASSAPDIIDPV
                     TGKERRAESRDIAQIAHLINELPGYDIFSISTLADDAPADQFTVSRLYPSIKYCLKPI
                     RVTTTNMKDTMSIMEMAYIVAGGEAAYKEHPFLTHHYCPVVSPLTMDHLSTENVMFFA
                     QQGLPVYPTIVPNAGLTSPMSMAGSLVQGNAEFLSALMLMQMTKEGTPTIYATLGTVA
                     DMRSGAYTSGAIECGMLHMAFAQMARFYDVPAGGYVGLTNSKLNDAQAGYETGMSGIA
                     GVLGGMDMFNIGGLIDALKTFDFAKAVIDDEIAQMMKRMKRGVSFSDEDLGVNLIKEI
                     GPGGSFITAKHTTSRMKTEAVMTKIADRDARSIWEKKGATDTHAKALKKAKDVMASNT
                     TPLISPEVDEKIRAAFPGLVPGVLEPIP"
     gene            2181..2801
                     /locus_tag="PKI33_04590"
     CDS             2181..2801
                     /locus_tag="PKI33_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNM36312.1"
                     /translation="MFRIFRRKEDKFQKLIEQQAEISFEGLKLLVKYLETSAPDVAEE
                     LSFKEKEADEVRRILIDELNRTFVTPFDREDIFSLSRSIDDVVDYADTTVVEMVILKV
                     SPTPYMQRIASLLKDAAYEIWQGVLRLPKHPNVALDHAQRAKALENRVEAVYREAVAD
                     LFSGPEDIHHVVEMLKLREVYRHLSNAADRGDEAANVIADIVVKRT"
     gene            3103..>3988
                     /locus_tag="PKI33_04595"
     CDS             3103..>3988
                     /locus_tag="PKI33_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM36313.1"
                     /translation="MRTKNFAYHLLALFVIAATLLAACGAPAPTEAPAAATDAPAATE
                     AAAATEAPAADPMADLVAAAQAEGTLTVIALPHDWCNYGEAIDTFKAKYGLEVNELNP
                     DAGSGDEIEAIKANKDNTGPQAPDVIDVGFAFGESSKAEGLIQPYKVSTWDSIPAEAK
                     DPEGYWYGDYYGVLSFLVNTDVQPDVPQDWADLLDPKYNGQVALSGDPRTSNQAIQTV
                     YAAALANGGSLDDAQAGLDFFAQLNSAGNLVPLISNNGLVAKGETPVRITWDYNALAA
                     VDSFAGNPAAQVVIPATGR"
BASE COUNT         1010 a          903 c         1004 g         1071 t
ORIGIN      
        1 tcaaatctgc aaaactgcta tatgtggatt ggtatgatgg cgatcatatc gtccgcgcga
       61 tggcagaagc taagaaacat aatgtgccgg tctttttgaa cttcgaacat ggacaccaga
      121 acccggaatt gctcaaaaaa tatgcgggta tggtcacggt ctgtcaggca gtgaccgatg
      181 cggcgcagat tggcaaacgt caagctatgt taggcacagc gcgcaagctc attaaatctg
      241 gtattcaaac agctatcatc actatggcaa gtcaggggtg tatggtggtg caaggtgatg
      301 aaattatccg cgcatatgcg cccaaggtta aggctgttga cgcctcagga gcaggcgcca
      361 cattttcatc tggctatatt tatggttatc tcaatggctg gagcttggaa gacacggttc
      421 gttttgcaat tgctgcagcg tctttaaaag tgacccgttc tgggttggat atgttcccag
      481 tatctgaaat caaaggattg gctcgcatgg ttcgtattga acgcatggtg tatcgcggag
      541 accaattccg tactattcgt aagttgttga cccttccaga gaacagtcca ttggttaaca
      601 atgcgctcgt gaaagaaagt cagaagctgg cggaaagaat tttgcctaag aagaaaatag
      661 agcgtaaaaa gatcaaacgc tcactggttg aataatttat tttataagga tgtgaaccat
      721 gtcatttgct gctttgctta cgcaagaaca agttgaaaaa attcacgatg cttcattaga
      781 gattttggaa gatgttggtt taaaagtccg ttatgaacct gcccgcgagc tattcaagaa
      841 gcatggctgt acggtagaag atgagcgcgt gaaatttcca cgtgctgttg tggaaaagta
      901 tcgcaaactg gttccgccct cttttacgtt tcacgcgcgt gacccgaaat ttgataagac
      961 catcccgcag gatagcccgg tcattgttac agcgagttcc gcgcccgata ttattgatcc
     1021 agtgaccggc aaagaaagac gcgccgaatc cagagatata gcgcagatcg cccacctcat
     1081 caacgaattg cccggatacg atatcttctc catttccacg ttggcagatg acgcacccgc
     1141 ggatcaattc acggtatcgc gcttgtatcc gtccatcaag tattgtctta aaccgatccg
     1201 tgtgactacg accaacatga aagacaccat gagcatcatg gaaatggcat acattgtggc
     1261 tggtggggaa gcggcatata aggagcatcc gttccttacg catcactatt gcccggtcgt
     1321 ttcgccgttg acaatggacc atctctcgac ggagaatgtg atgttctttg cacaacaagg
     1381 gctgcctgtt tacccaacca ttgtgccgaa tgccggtctt acatcgccca tgtctatggc
     1441 tggtagtttg gtgcaaggta atgctgaatt tttatctgcg ctgatgttga tgcagatgac
     1501 aaaggaaggc acaccgacga tctatgcaac actgggcaca gtggctgata tgcgttcggg
     1561 tgcatatacc tctggcgcga ttgaatgtgg catgttgcat atggcatttg ctcagatggc
     1621 gcgtttttat gatgtgcctg cgggcggata tgttggcttg acgaactcca aactgaacga
     1681 tgctcaggca gggtatgaaa ccggtatgtc tggcattgcg ggagtgctcg gcggtatgga
     1741 tatgttcaac attggcggtt tgatcgatgc attgaagacc ttcgattttg ccaaagcagt
     1801 gatcgatgat gaaattgcac agatgatgaa gcgcatgaaa cgcggcgtca gctttagcga
     1861 tgaagatttg ggagtcaatc tcatcaaaga gattggccct ggcggctcct ttatcacagc
     1921 caaacacact accagccgca tgaagaccga agcggtgatg acaaaaatcg ctgaccgtga
     1981 tgctcgttcc atttgggaga agaaaggcgc tacggataca catgccaagg cgttgaagaa
     2041 agccaaagat gttatggctt ccaataccac tcctctgatc tcgcccgaag tggatgaaaa
     2101 gattcgcgct gcattccctg gcttagtgcc cggggtgtta gagcctatcc cgtaatacaa
     2161 ggaataaaag aaggatttga atgtttcgta tttttaggcg caaggaagat aaattccaaa
     2221 aactgattga gcaacaagcg gaaatatcgt ttgaaggctt gaagttattg gtgaaatatc
     2281 ttgaaacgtc ggctccagat gtagctgaag aactgtcgtt caaagagaaa gaggcggatg
     2341 aagttcgtcg catcttgatc gatgaactga accgcacatt tgtcacaccg tttgaccgtg
     2401 aagatatctt ttcgctctct cgttcgatcg atgatgtggt tgattatgcc gatacaactg
     2461 ttgtagaaat ggtgattcta aaagtcagcc caacacctta tatgcagcga attgcatctt
     2521 tgttgaagga tgctgcttat gagatttggc aaggcgtgct gcgtttgcct aagcacccaa
     2581 atgtggcatt ggatcatgca caacgtgcca aggcgcttga aaatcgtgta gaagcggtat
     2641 accgtgaagc agttgcagat ctgtttagcg gacctgaaga tattcatcat gtggttgaga
     2701 tgcttaaact ccgtgaggtg tatcgtcatc tttctaatgc ggctgatcgc ggcgatgaag
     2761 ctgccaatgt gattgcagat attgttgtaa aaagaactta accggatgtt aatccctttt
     2821 ctttttgagt ttgttattat ccttgcgctg ataattgaat ttagtaatga tgcgcgcaac
     2881 attcaaagtg gtaataccga tatcttgtat gtgactccca tgtttattgg tacaaataac
     2941 tacgttggtc attaattcaa agtctcacaa gcttgtttga ctttgaatat tgataatgct
     3001 aaaatgttaa cgatttgtta atggaatgga ggtgccggga agttaagata gcgcaagtat
     3061 tccgttcgtt cgatcaatct aaaatatcat aaggagaaag agatgcgtac gaagaatttt
     3121 gcttatcatt tgctcgccct gttcgtgatc gccgctacct tgttggctgc ttgtggtgct
     3181 cctgctccca ctgaagcccc tgctgctgcg accgatgccc ccgctgccac cgaagctgct
     3241 gccgccactg aagcccctgc tgccgacccc atggctgacc tcgttgctgc tgcgcaggct
     3301 gaaggaactt tgaccgtgat tgccttgccc catgactggt gcaattatgg tgaagccatt
     3361 gacaccttca aggcgaaata tggtctcgaa gtcaatgaac tcaatcctga tgctggctcc
     3421 ggtgatgaaa tcgaagctat caaggcgaac aaagacaaca cgggtcctca ggctccggat
     3481 gtgatcgacg ttggttttgc tttcggtgaa tcttccaagg ctgaaggttt gatccagccg
     3541 tacaaggttt ccacctggga ttccattcct gctgaagcca aggatcctga aggctactgg
     3601 tatggcgact actatggtgt tctttccttc ctcgtcaaca ccgatgtcca acccgatgtt
     3661 cctcaggact gggctgactt gctcgatccc aagtacaacg gacaagttgc tctctctggc
     3721 gatcctcgca catccaatca ggctatccag accgtctatg ctgctgccct tgctaacggt
     3781 ggctccctcg atgacgctca ggctggtctc gacttctttg ctcagttgaa cagcgctggt
     3841 aaccttgttc ctttgatctc gaacaacggt ttggttgcca agggtgaaac ccccgtccgc
     3901 attacctggg actacaatgc cctcgccgct gtggattcct ttgctggcaa tcctgctgct
     3961 caggttgtga tccccgctac tggccgct
//
LOCUS       DAOREF010000163         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13677_length_3984_cov_3.780606, whole genome shotgun sequence.
ACCESSION   DAOREF010000163 DAOREF010000000
VERSION     DAOREF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13677_length_3984_cov_3.780606"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKI33_04600"
     CDS             <1..190
                     /locus_tag="PKI33_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36314.1"
                     /translation="EDGVDFERTQHSEIFRMSKEEQVDGSALAQTLDKLSDLALHSRT
                     SDLIQTINHFLPSGSVQS"
     gene            187..1170
                     /locus_tag="PKI33_04605"
     CDS             187..1170
                     /locus_tag="PKI33_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNM36315.1"
                     /translation="MTEVALAEWNQFLTKFPHTHLLQMGEWGELKKDFGWKPIRIISE
                     NVGVQILFRRLPLGFTIAYMPKVASDQLSTTSDQFWREVDSVCKKNRAIFLKLEPDTW
                     TDEFITHHSSFIISKHNIQPPRTITLDINASEENILSRMKPKCRYNIRLAEKKGVTIR
                     AWGDIPAFYEMMTVTGGRDGFGVHSKEYYQRAYELFHPKGTCELLVAEYEGKPLASLM
                     VFANAKRAWYVYGASNNEERNRMPTYLLQWEAIRWAKARGCEEYDLWGVPDEDEETLE
                     AQFESRHDGLWGVYRFKRGFGGQLKRAVQALDRVYNPLLYKIYLWRFSGGE"
     gene            1173..1460
                     /locus_tag="PKI33_04610"
     CDS             1173..1460
                     /locus_tag="PKI33_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36316.1"
                     /translation="MSYFDVIQEIEKLPHYREFVCSACGYKQRVLVLIIDYKCEKCGE
                     NLKLRGHAPIGTEIEDVIDAVLEWMGKGTKLNDVMEWKNIRDSFPIDGTNE"
     gene            1453..2598
                     /locus_tag="PKI33_04615"
     CDS             1453..2598
                     /locus_tag="PKI33_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNM36317.1"
                     /translation="MSNNFWNDLISKLPNPHFLQTYEWGQVKAKYGWVPYYAVWTEDG
                     KWKVESDVNSLSTFHSPIVAASLILKRTAFRRFSIFYAPKGPLMDWTNESLRKRVLDD
                     LQSFAKKQGAIFLKLDPDVVLGRGVPASVGEVTENSGQAVMSDLRRRGWVDSSDQIQF
                     RNTVMVDLSASEEDILMRMKQKTRYNVRLAEKKGVTVRVGSVDDLPMLYKMYAETSVR
                     DGFVIRDEEYYMMVWKLFLTNYESRITPSTIPLIAEVDNAPVAAIFLFTFAARGYYVY
                     GMSRDKHREKMPTYLLQWAAMKHTKAHNCLTYDLWGAPDVFDESDSMWGVYRFKEGLG
                     GEVVRTLGAYDFAPSKFWYSMYADVMPRVLNFMRSRGKEKTKQGLSA"
     gene            2679..3650
                     /locus_tag="PKI33_04620"
     CDS             2679..3650
                     /locus_tag="PKI33_04620"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNM36318.1"
                     /translation="MNKIARLNFAHLPTSIEELPNLSKALNGPHIFVKRDDQTGLAFG
                     GNKTRKLEFLIAEAREQGADLLISGGAMQSNHCRQTAAAAARYGFECKLVLTGAKPQQ
                     ASANYLLDQLFGAEIITVEDRAFRDQTLQKTFDDALAAGKKPYLVPYGGSSPTGAMGY
                     TFAMEEFMQQNAHADWIVFGTSSGGTHAGLVLGQRLFGYKGKVLGISIDEPEEELKSH
                     VSDLASRASEKLGERIQFSSNDVLATEDYCQSGYGVFGEGEREAIHLFAKHEGLLLDP
                     VYTGRAAAGMIDLIRKGFFKKDEMILFWHTGGQPALFADKYAQDVIR"
     gene            complement(3700..3936)
                     /locus_tag="PKI33_04625"
     CDS             complement(3700..3936)
                     /locus_tag="PKI33_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36319.1"
                     /translation="MDLIDILGWIGSATYLLAFALNSAKKVTSDSFTYQGMNILAGVI
                     MTYYTFSHGAYSATALNSIWAIIGVVTLVNKGTR"
BASE COUNT         1040 a          974 c         1006 g          964 t
ORIGIN      
        1 cgaagacggc gtggattttg aacgcacgca acactcggaa atttttcgca tgtcgaaaga
       61 ggaacaggtg gacggctccg cgcttgctca aactttggac aaactctcag acctcgcgct
      121 tcactcccgg acctctgacc tgattcagac catcaaccat ttcctgccct ccggctcggt
      181 gcaatcatga ccgaagtggc gcttgctgaa tggaatcaat tcctaactaa attcccccac
      241 acgcatctct tgcagatggg cgaatggggt gaattaaaaa aagattttgg ctggaagccc
      301 atccgcatta tctctgagaa tgttggcgta caaattttgt tccgccgcct gccgctggga
      361 ttcacgatcg cgtacatgcc gaaagtagcc agtgaccagt tatcaacgac cagtgaccag
      421 ttctggcgtg aagttgattc tgtttgtaaa aagaaccgtg ccatctttct aaaactcgaa
      481 cccgacacat ggacagatga attcatcact catcattcat cattcatcat ttccaaacat
      541 aacatccaac ctccccgcac catcacccta gacatcaacg ccagcgaaga aaatattctg
      601 tctcgaatga aacccaaatg ccgctacaac attcgcctcg ctgagaaaaa gggcgtgacc
      661 atccgcgcat ggggcgacat ccccgccttc tacgagatga tgaccgtcac cggcgggcgt
      721 gatggttttg gtgtacattc caaggaatat tatcaacgcg cctacgaact cttccaccca
      781 aaagggacgt gtgaactgct cgttgcagaa tacgagggta aaccgctggc ttcgctgatg
      841 gtcttcgcaa atgccaaacg cgcttggtat gtgtatggcg cgtcgaacaa cgaagaacgc
      901 aaccgcatgc cgacatatct cttgcaatgg gaagccatcc gctgggcaaa ggcacgcggc
      961 tgtgaagaat acgacctgtg gggtgtcccc gacgaggacg aagaaacact cgaagcccaa
     1021 ttcgagtcgc ggcacgatgg cttgtggggc gtctaccgtt tcaaacgcgg ctttggcggt
     1081 caattgaaac gtgccgtgca ggcattggat agagtttaca atccgctttt atataaaata
     1141 tatctgtggc gattttctgg aggcgaataa ttatgtctta ttttgatgta attcaggaga
     1201 ttgaaaagtt gcctcattac cgcgaatttg tttgtagcgc atgtggttat aaacaaaggg
     1261 ttttagtttt gataatcgat tacaaatgtg aaaagtgtgg cgaaaatttg aaattaagag
     1321 gacatgctcc aataggaact gaaatagagg atgttattga cgcagtacta gaatggatgg
     1381 gaaagggaac aaaacttaac gatgtgatgg agtggaaaaa tataagagac tccttcccga
     1441 ttgacggaac taatgagtaa taacttttgg aatgacttaa tttcaaaatt acctaatccg
     1501 cacttcctgc aaacctatga atggggacag gtcaaagcga agtatggctg ggttccatat
     1561 tacgccgtct ggacagaaga tggaaagtgg aaagtagaaa gcgatgtcaa ttcactttcc
     1621 actttccact caccaatcgt cgctgcttca ctcattctca aacgtaccgc tttccgtcgt
     1681 ttctccatct tctacgctcc caaaggtccg ctcatggatt ggacgaatga gtccctgcgt
     1741 aaacgcgtgc tggatgactt gcaatccttc gcgaagaaac aaggcgcaat cttcctgaaa
     1801 cttgaccccg atgtggtgtt ggggcgcgga gtccctgcca gcgtggggga ggtcacagaa
     1861 aacagcggac aggctgtgat gtccgatctg aggcgcaggg gctgggtgga ttcgtccgac
     1921 cagatccagt tccgcaatac cgtcatggtc gatctatctg ccagcgaaga agatatcctc
     1981 atgcgcatga aacagaagac gcgttacaac gtgcgcctcg ctgagaaaaa aggcgtgacc
     2041 gtgcgcgtgg gcagcgtgga cgatttgccg atgttgtaca agatgtatgc tgaaacatca
     2101 gtgcgtgatg gctttgtgat tcgtgatgaa gaatattaca tgatggtttg gaaattgttc
     2161 cttacgaatt acgaatcacg aattacgcct tcaaccattc cattaattgc ggaagtagac
     2221 aacgcgcccg tcgctgctat tttcctcttc accttcgccg cgcgcggata ttatgtctac
     2281 ggcatgtcgc gggataaaca ccgtgaaaag atgccaacct atcttttgca atgggcggcg
     2341 atgaaacata ccaaggcgca caactgcctg acctatgacc tgtggggcgc gccggatgtc
     2401 ttcgatgaaa gcgactcgat gtggggcgtg tatcgcttca aggaaggttt gggtggtgaa
     2461 gtggtgcgta cgctcggtgc gtatgacttt gcgccgagca agttctggta ttcgatgtac
     2521 gccgatgtca tgccgcgcgt gttgaatttt atgaggtcgc gtggcaaaga aaagacaaag
     2581 caaggattga gtgcgtaaaa cgtgatgcgt aatacgtaat tcgtaaatca cgtttcactc
     2641 ttcacgtatt acgcattacg gcaaaaaagg acctaaccat gaacaaaatc gcccgcttaa
     2701 acttcgccca tcttcccacc tcaattgaag aacttcccaa cctttccaag gccctgaatg
     2761 gaccgcatat cttcgtcaag cgggatgacc agaccgggct ggcattcggt ggcaacaaaa
     2821 cccgcaaatt ggaattcctc attgccgaag cccgagaaca aggcgcagat ttgcttattt
     2881 cgggcggagc gatgcagtcc aatcactgcc gtcagaccgc ggcggcagcg gcacgatatg
     2941 gatttgaatg taagttagta ttgaccggcg cgaaaccgca gcaagcctct gctaattatt
     3001 tactcgacca actcttcggc gcagagatca tcaccgtgga agatcgcgcc ttccgcgacc
     3061 agactctgca aaagacgttt gatgacgcgc ttgccgccgg gaagaaaccc tatcttgttc
     3121 cgtatggcgg ttccagtcca accggcgcga tgggctacac ctttgcgatg gaagagttca
     3181 tgcagcagaa cgcccatgcc gattggatcg ttttcggtac atcctctggc gggactcatg
     3241 ccgggctggt gttgggtcag cgtttgtttg gctataaagg caaagtgttg ggaatcagca
     3301 ttgacgagcc cgaagaagaa ctgaagtcac atgtctctga tttggcttct cgcgccagtg
     3361 aaaaacttgg tgagcggatt caattctcaa gcaacgatgt gctggcgaca gaagattatt
     3421 gccagtcagg ctatggcgtc tttggtgaag gcgagcgcga agcaattcac ttgttcgcca
     3481 agcatgaagg cttattgctc gaccccgtct acaccggcag agccgccgca gggatgatcg
     3541 acctgatccg caaaggattt ttcaagaaag acgagatgat cttattctgg cataccggcg
     3601 gtcagcccgc gctgtttgcg gataagtacg cgcaagatgt gatacgttaa ttttagaccg
     3661 cggacggctg acaattgcct gccgtccacg gtctgctatt taccgtgtgc ccttattcac
     3721 cagcgtcacc actccaatga tcgcccaaat ggaattgagt gcggttgccg aatatgcgcc
     3781 gtgactaaag gtgtagtagg tcatgatcac acctgctaga atgttcatgc cttgataggt
     3841 gaatgagtca ctggttactt ttttggcaga atttaacgca aatgccaaaa gataagttgc
     3901 cgaaccgatc cagccgagaa tatcaattaa gtccattatt ccaaaatctc cacggtcatt
     3961 ccaatttcaa tttttccctc gttc
//
LOCUS       DAOREF010000164         3961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13791_length_3961_cov_3.597030, whole genome shotgun sequence.
ACCESSION   DAOREF010000164 DAOREF010000000
VERSION     DAOREF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13791_length_3961_cov_3.597030"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKI33_04630"
     CDS             <1..354
                     /locus_tag="PKI33_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HNM36320.1"
                     /translation="NAEAVDFPDFTKKVGEKVQSGEAERGILICGSGIGAAIAANKMK
                     GIFASICHDTYSAAQGVEHDAMNVLCMGGRVIGPELVKVIVPAFLNAEYLGDKTGGER
                     FARRVGKIRKMEENQ"
     gene            411..3101
                     /locus_tag="PKI33_04635"
     CDS             411..3101
                     /locus_tag="PKI33_04635"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HNM36321.1"
                     /translation="MSESIKKLTALGQSLWYDNIQRKLLVNGELKAMINRGDIRGVTS
                     NPTIFQNAIAKTNDYDAALVPLAWAGWDAEKIFWQLAVEDIQEACDLFRPLYDESKGG
                     DGYVSIEVSPFLARDTEGTIKQAQELWERVNRPNLMVKIPATKEGVPAIRASIAAGIN
                     VNVTLIFSLSRYAEVMNAYLEGIEERVGKDLPVQHVASVASFFVSRVDTKVDPKLPED
                     SPLRGKAAVANAKLAYEQFEAIFTSPRFATLKARFRARVQRPLWASTGTKNPKYSDTL
                     YVDELIGPDTVNTVPPATLDAFRDHGTATMTITRDLDSMQKIFVDLKALGISMSTVTQ
                     ELEDEGVKAFDDAFKSLLDSIETRRKAALASIAPLADAVAKRLATLEAESFSKRLWEH
                     DVTLWSGADDPEGQAEAAKRLGWLDSVDDARARLDGYLSFAKQVHDEKIDRVLVTGMG
                     GSSLTAEVLSSLLANFGITAKLSLAILDSTDPQQVAQAVKDYSPDKSLYIIASKSGGT
                     AELMAAFDYLWELSKHDGSRFIVTTDPGTSLEKMANERGFRKVFNADPMVGGRFSALT
                     DFGLVPAALMGMDFDIFLGNADRMRSQSLAHVPAARNPGVALGALIAESALMGRDKLT
                     VLADAPVSAMAGWVEQVIAESSGKHGKGILPVALEPLGKPEDYGNDRLFVYIKSNGEL
                     EAGISELKKAGFPVIEFPMESAYDASTEFFRWEIAISTACHILGINAFDQPDVQDSKL
                     RTIAKIKDYQSTGKLAETDLVDLKDAKKALEEFLSKPQAGEFVTINAYLPRNGEMIEA
                     IQTLRTNIRAKTKLPVSAGFGPRFQHSTGQFHKGGPNKGRFIQFVYDAETDMDIPGQG
                     LSFGTLIRAQALGDYEALKAAGRKALRIRLSKPEDIKGLF"
     gene            3101..3436
                     /locus_tag="PKI33_04640"
     CDS             3101..3436
                     /locus_tag="PKI33_04640"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNM36322.1"
                     /translation="MALSDGKCIPCRKGDPALTNAEVTELLSQIPAWELVEVDGIRRL
                     QRVFKLKNYAEAVAFTNKLAMTAEKEDHHPLIVLEWGRVTVQWWTHVVQGLHQNDFVM
                     AAKTNEIFG"
     gene            complement(3467..>3961)
                     /locus_tag="PKI33_04645"
     CDS             complement(3467..>3961)
                     /locus_tag="PKI33_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36323.1"
                     /translation="HEWENYRNETLLYNFATPGTKNAIRTVVHRLWLKPLVHIDPDVE
                     YFESKENSLVDEHKQQLQDLALICDFKATSDLPQWMTAQEREKVRAFLDAETDSVQIS
                     RYVYKLDNRIVDFSSAVELPAPPTGFASAWGAFLGWLGNWKFVLRIIKMMDDKNLKER
                     VSRI"
BASE COUNT          984 a          909 c         1040 g         1028 t
ORIGIN      
        1 aatgctgaag ctgtggactt tccggatttc acgaaaaaag ttggtgagaa agtgcaaagc
       61 ggcgaagccg agcgcggaat tttgatttgc ggttcgggca ttggtgccgc gattgctgcc
      121 aataaaatga aaggcatttt cgcttcgatc tgccacgaca cgtattcagc cgcacaaggt
      181 gtggagcacg atgccatgaa tgtgctttgt atgggtgggc gtgtgatcgg accggaactg
      241 gtaaaagtta ttgtgcctgc atttttgaat gcagaatatt taggggacaa aacgggtggt
      301 gaacgctttg cgcgccgggt gggaaagatt aggaaaatgg aagagaacca gtaaatagta
      361 attggtaatt accagttact atttaattaa tttaaggagc attttttatt atgtctgaat
      421 caattaagaa attaactgcc cttgggcaat cgctttggta cgataacatc caacgtaaac
      481 ttttggtcaa cggtgaactc aaagctatga tcaatcgtgg cgatattcgc ggtgtgacct
      541 ccaacccgac tattttccaa aatgccattg caaaaacgaa tgattatgac gcggctctcg
      601 ttccactggc gtgggcaggt tgggatgccg agaagatttt ctggcaactt gcggtagaag
      661 atattcaaga agcttgtgac ctgttccgcc cgttgtatga tgaaagcaaa ggcggcgatg
      721 gctatgtgag cattgaggtc agcccatttt tggcgcgaga taccgaaggg acgattaagc
      781 aagcacagga actttgggaa cgtgtcaatc gaccgaattt gatggtgaaa attcccgcca
      841 caaaagaagg tgtgcccgcc attcgtgcca gcattgctgc gggaattaac gtcaacgtta
      901 ccttgatctt ttccctctcg cgctatgctg aagtgatgaa tgcctatctc gaaggcattg
      961 aagaacgtgt gggcaaagac cttcccgttc aacatgtggc atcagttgct tcgttctttg
     1021 tctcccgggt tgataccaag gttgacccaa aacttcccga agattctccc ttgcgaggga
     1081 aggctgcggt tgccaatgcc aaacttgcct atgaacaatt cgaagcgatc ttcacctcgc
     1141 cgcgttttgc cacactcaag gctcgtttcc gcgcacgcgt gcaacgtccg ctgtgggcat
     1201 ctactggcac gaaaaatccg aaatactctg atacgttata tgttgatgaa cttatcggtc
     1261 ctgatacggt caatactgtg ccgcctgcca cgctcgatgc tttccgcgat catggcactg
     1321 caactatgac cattacgcgt gacctcgata gcatgcagaa aatttttgta gacctcaaag
     1381 ctcttggcat ttctatgagc acggttacac aagaacttga agatgaaggt gtgaaagcgt
     1441 ttgatgatgc tttcaagtct ttgttggatt ctattgaaac aagacggaaa gctgcgctcg
     1501 cctcgatcgc gccgttggct gatgctgttg ccaaacgttt agccacgctg gaagctgaat
     1561 ctttctccaa acgcctttgg gagcatgatg tcaccctctg gtctggcgca gacgaccctg
     1621 aaggtcaagc tgaagccgcc aaacgtttgg gctggttgga ctctgtcgac gatgctcgtg
     1681 caaggcttga cggatatctt tcatttgcca agcaagtcca cgatgaaaag attgaccggg
     1741 tcttggtcac tggtatgggc ggctcctcgt tgactgcaga agtgctcagc tcattgctag
     1801 caaactttgg cattacagcc aaactctcgc ttgccattct cgactcgact gacccgcaac
     1861 aagtggcgca ggcggtcaaa gattattctc cagataaatc actgtacatc atcgccagca
     1921 aatctggtgg gactgccgaa ctgatggcgg ctttcgatta cctgtgggag ctgagcaagc
     1981 acgatggctc acgtttcatc gtcaccaccg accctggtac ctcgctcgaa aaaatggcga
     2041 acgaacgcgg cttccgcaaa gtgttcaacg ctgacccgat ggtgggtggg cggttctccg
     2101 cgttgaccga tttcggtttg gtccccgctg cattaatggg catggatttt gacatattcc
     2161 ttggcaatgc agaccgtatg cgtagccagt ctttggcgca tgtgcctgct gcacgcaacc
     2221 cgggtgtggc gttaggcgct ctcattgcag aatccgcttt gatgggtaga gacaagctca
     2281 cagtcctggc tgatgcgcct gtatctgcta tggcaggttg ggtggagcag gtcattgccg
     2341 agtctagcgg caagcatggc aagggcatcc tgcctgttgc gttggaaccg ctaggcaaac
     2401 cggaagatta tggcaatgac cgcttgttcg tgtacatcaa gagcaatggc gaattggaag
     2461 ccggcatttc tgagttgaag aaagcgggct ttcctgtgat cgaattcccg atggaaagcg
     2521 catacgatgc cagcactgaa tttttccgtt gggagattgc gatttcaaca gcatgccaca
     2581 ttcttggaat caatgcgttt gaccaacccg atgtgcaaga tagtaaattg cgtacgattg
     2641 ccaagatcaa agattatcaa tccacgggca aacttgctga aaccgattta gtggatctca
     2701 aggatgccaa gaaagcgctt gaagaatttt tgtccaagcc gcaagctggc gagtttgtga
     2761 ccatcaatgc ttatttgcca cgtaatggcg agatgataga ggctattcaa accttgagaa
     2821 caaatatccg cgccaagacc aagttgcctg tttctgctgg ctttggtccg cgctttcagc
     2881 attcgacggg tcagttccac aagggcggac ccaataaagg acgcttcatt cagtttgtgt
     2941 acgatgctga aaccgatatg gatattcccg ggcagggctt gagttttggt acgctcatcc
     3001 gtgcgcaggc attgggtgat tatgaagcat tgaaagcggc gggacgtaaa gcgttgcgca
     3061 tccgcctctc taagccggaa gatatcaaag gtttgttcta gtggctcttt cggatggaaa
     3121 gtgtattcca tgccgaaaag gtgacccagc gttaaccaac gctgaggtca ctgaactgct
     3181 ttcacaaatc cctgcatggg aattggtgga agtagatggc atccgacgtt tacagcgagt
     3241 ctttaaactg aaaaactatg ctgaggctgt tgcttttaca aataagctcg caatgaccgc
     3301 tgaaaaagaa gaccaccatc cactgattgt gctggaatgg ggcagagtta ccgtccaatg
     3361 gtggacgcat gtggttcaag gcttgcatca gaacgatttt gtgatggcag ccaagacaaa
     3421 tgaaattttc ggataataaa agcgacccat cagggtcgct tttattttat atccttgaaa
     3481 ctcgttcttt taagttttta tcatccatca tttttatgat tcgcagcacg aacttccagt
     3541 taccaagcca accgaggaat gcgccccatg cacttgcaaa gcctgtaggc ggcgcaggca
     3601 gttctacggc ggatgaaaag tccacgatgc ggttatcgag tttgtaaacg taacggctga
     3661 tttgcaccga gtcggtttca gcatccagaa atgcgcggac tttttctcgc tcctgagccg
     3721 tcatccattg aggtaagtcg gacgtcgctt tgaagtcgca gatcagggcg aggtcttgca
     3781 attgctgttt gtgctcatct accaaagaat tttcttttga ttcaaaatat tccacatcgg
     3841 ggtcaatgtg aacgaggggc ttgagccaca agcggtgaac cacggtgcga atggcgttct
     3901 ttgtacctgg tgtggcgaag ttgtacaaga gagtttcgtt acgatagttt tcccattcat
     3961 g
//
LOCUS       DAOREF010000165         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13818_length_3956_cov_2.900538, whole genome shotgun sequence.
ACCESSION   DAOREF010000165 DAOREF010000000
VERSION     DAOREF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13818_length_3956_cov_2.900538"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..823
                     /locus_tag="PKI33_04650"
     CDS             92..823
                     /locus_tag="PKI33_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNM36324.1"
                     /translation="MEISQITVWLALLAGLASFLSPCVFSLVPAYVGYLGGIAAGSGT
                     PGEKPNRWLTFSHGVAFVLGFSIVFVLLGVAASFAGGLLYDLRYWLAKIGGVVVIIFG
                     LHMIGVFHIPFLAYDTRVQKAPDPKLGYFSSALMGVFFSAGWSPCVGPVLGAILTLAI
                     NGGSISQGATLLTFYSAGLAIPFLLAALGIGWVTTILRKYNKTMRYVEIAMGVILIII
                     GIMLFAGTFELIAQRGQFFFIDFGL"
     gene            881..1918
                     /locus_tag="PKI33_04655"
     CDS             881..1918
                     /locus_tag="PKI33_04655"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNM36325.1"
                     /translation="MKPAKGLSHMLNVTLFTKKDCKLCDEVKADLKALQEQYPHRLAE
                     VDIESDAALYEKFRNVIPVIEVGPYTVQAPISRQKLQMTLGAASDRKNQLEKLDDPVY
                     KYKMEKGKKLSTGDRVSFWISKRYLLLLNLFMLLYVGLPFLAPTLMKLGAEAPAQLIY
                     RIYKPLCHQFAFRSFFLFGEQPFYPLAEANLTGYKTFEQATGIAGVDDPYNMARFEAR
                     NYIGDSTIGYKVALCERDVAIYLAILAFGIIFGLTGRRFKSVHWLVWILIGIAPIGLD
                     GFSQLFSQFNWDWLNTLLPYRESTPFLRVLTGALFGLFTAWFAYPNIEESMNETRQYF
                     LKKSAVIEAAE"
     gene            1918..2697
                     /gene="pgeF"
                     /locus_tag="PKI33_04660"
     CDS             1918..2697
                     /gene="pgeF"
                     /locus_tag="PKI33_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNM36326.1"
                     /translation="MPFDQSDGLRYYQFDIFSKNILNAVFTRQGGISPEPWTSLNLSI
                     SVGDDPARVAENRVHAFNALGRNPASIHDAWLIHGTDVIFADAPRSLDAPTQKADILF
                     TDNPEVSLFMRFADCVPLLFHDPKKNVIGISHAGWMGTVKGVAEVSVQAMQERYGSNP
                     QDIVVGIGPSISADHYEVGEEVAAQFREKYGRDSEQVVQVRDEKIYLDLWAANALQLQ
                     KMGVEHIQISGLCTACHLDDWYSHRAEKGKTGRFGVLLAMV"
     gene            2727..3470
                     /locus_tag="PKI33_04665"
     CDS             2727..3470
                     /locus_tag="PKI33_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNM36327.1"
                     /translation="MSELVNSIRERYLSALDTIASAANRSGRSPEAVKLVVVTKFQPL
                     EVVQATIEAGAVILGENYAEEGVTKIQSLQNLSAVEWHMIGHVQSRKAPLVAGHFNFM
                     HSLDSLKLAKKLDRFCGEAGRVLPVLLEFNVGGEDSKGGWEAVDETRWEELLPEVSEL
                     LAIPNLQVRGLMSMPPLGMDAEFSRPYFQKLRRLQEFFTSHLPQATWIELSMGTSSDY
                     EVAVEEGATFVRIGTAIVGPRNYQVAENL"
     gene            3467..3721
                     /locus_tag="PKI33_04670"
     CDS             3467..3721
                     /locus_tag="PKI33_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNM36328.1"
                     /translation="MNGNTIISIIIIVADFFVWVVIASSLLSFFLPPYHPVREALGKI
                     VDPFLNPIRSLMPSGGGLDFSPLILILIIELITNFLRSLL"
     gene            3731..>3956
                     /locus_tag="PKI33_04675"
     CDS             3731..>3956
                     /locus_tag="PKI33_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNM36329.1"
                     /translation="MSREYVLHDGKRGSALAVRITPRASRNQIAGVLNDGTVKIQLSA
                     DAEDKNMNSDLLAFLAEVLGVPRTRVEIVAG"
BASE COUNT          896 a          937 c         1007 g         1116 t
ORIGIN      
        1 cgtttctctc gctgctggcg aagaacgccg cgaactgcca gaccacggca ccacagtcac
       61 ttctaccgaa acccaattct aagaggcgtc catggaaatt tcccaaatta ctgtttggct
      121 tgctttgctt gccgggttgg cttcttttct ttcgccttgt gtattttctc ttgtcccagc
      181 ctatgtggga taccttggcg gcattgcagc aggcagtgga actccgggtg aaaagcccaa
      241 tcgttggctt accttttcac atggagttgc tttcgtacta gggttcagta tcgtgttcgt
      301 gttgctcggt gtagcggctt cttttgcagg cggtttgtta tacgatttac gctattggtt
      361 ggcgaagatc ggtggcgtgg tcgtcatcat cttcggtttg cacatgatcg gtgtctttca
      421 cattccgttc cttgcgtatg acacccgtgt tcaaaaagca ccagacccca agctcggata
      481 tttttcgtcc gcattgatgg gcgtattctt ctctgccgga tggtctccct gtgtaggacc
      541 tgtcctcggt gcgattttga ctctcgccat taacggcggc tcgatctctc agggcgccac
      601 gcttcttact ttctattcgg cgggtcttgc catcccattc cttctcgccg ccttgggcat
      661 tggttgggtc accaccattc tccgcaaata caacaagacc atgcgctatg ttgagatcgc
      721 catgggcgtc atcctgatca ttatcggtat catgctcttt gcagggacgt tcgaactcat
      781 cgcccaacgc ggacagttct tcttcattga tttcggtcta taatccccaa gacatatttc
      841 gtttttactc ccgcaggtag ccattcctgc gggaattatt ttgaaacctg caaagggact
      901 ttcgcatatg ctcaatgtca cactcttcac gaaaaaagat tgcaagctct gtgatgaagt
      961 caaagccgac ttgaaggcac tacaagaaca atatccgcac cgcttggcag aagtggacat
     1021 tgaatcagat gcggcgttgt acgaaaaatt tagaaatgtt atcccagtta tcgaagtcgg
     1081 accctacacc gttcaagcgc ccatctcgcg ccagaaacta caaatgactc tgggtgcagc
     1141 ctccgaccgt aaaaatcagc tcgaaaaatt ggacgacccc gtttataaat acaaaatgga
     1201 gaaaggaaag aaattgtcta ctggagaccg tgtttcattc tggatatcaa agcgttattt
     1261 gcttttgttg aatctgttca tgttgttgta tgtagggctg ccttttcttg cgccaaccct
     1321 catgaagttg ggcgccgaag cacccgcgca actgatctat cgcatctata aacctctttg
     1381 ccatcaattt gcttttcgtt ctttcttctt gtttggcgaa cagccttttt atccgctggc
     1441 agaggcgaac ctcacaggct ataaaacctt tgagcaagcc accggcattg caggtgtaga
     1501 tgatccatac aatatggctc gcttcgaagc ccgcaactat attggtgatt caaccattgg
     1561 ttacaaagtg gcgctttgcg aacgcgatgt cgcgatctat cttgctattc tggcgttcgg
     1621 catcatcttt ggactcaccg gtcgccgctt caagtctgta cattggctgg tgtggatttt
     1681 gatcggcatt gcgcccatcg ggttagacgg cttttcgcaa ctcttcagtc agttcaattg
     1741 ggattggctc aatacacttc taccgtatcg cgaaagcaca cccttcttac gtgttcttac
     1801 cggcgcgctc tttggtctct ttacggcttg gtttgcttat cccaacatcg aagaatccat
     1861 gaacgaaacc cgccagtatt tcctcaaaaa gtcagcagtg atcgaggcgg ctgaataatg
     1921 ccctttgacc aatcagacgg gctgcgttat taccagttcg atatcttttc aaagaatatc
     1981 ctcaatgcgg tgtttactcg tcagggcggc attagccccg aaccgtggac ttctctcaac
     2041 ttaagcattt cggttggaga tgatcccgcg cgcgtggctg agaaccgagt ccatgctttt
     2101 aatgcgcttg gacgaaaccc cgcctctatc cacgacgcat ggctgatcca cggcacagac
     2161 gttatcttcg cggatgcccc gcgctcgctc gatgctccga ctcaaaaagc agatatcctt
     2221 tttaccgaca accctgaagt ttcgctcttc atgcgctttg cagattgtgt ccctttgctt
     2281 tttcacgacc cgaagaaaaa cgtaatcggc atttcacacg caggttggat gggaactgtg
     2341 aaaggagtcg ctgaggtctc tgtgcaagcc atgcaagaac gatacggttc caatccgcaa
     2401 gatattgtgg tcggcattgg tccgtctatt agtgcagatc attatgaggt aggtgaggaa
     2461 gttgctgcgc aattccgtga gaaatatggc agagactctg aacaagttgt tcaggtgcgg
     2521 gatgagaaga tttatcttga tttgtgggcg gcaaatgctt tgcaattgca gaagatgggt
     2581 gtggagcata ttcagatatc gggcttatgc actgcctgtc atttagacga ttggtattca
     2641 caccgtgccg aaaaagggaa aacaggtcgt tttggcgtgt tgttggcaat ggtttgagaa
     2701 aaccctgggt gggttaaaat aatcatatga gcgaattggt caattctata cgagagcggt
     2761 atctgtcggc gttggatacg atcgcgtcag cggcgaacag gtctgggcgg agtccggagg
     2821 cggtgaagtt ggtcgtcgtc accaaattcc agccgctgga ggtcgttcag gcgacgattg
     2881 aggcaggggc ggtcatcttg ggcgaaaatt atgccgaaga gggtgtcacg aaaattcaat
     2941 ctttgcaaaa tctttctgcg gtagaatggc acatgatcgg tcacgtgcag agccgaaagg
     3001 caccgctggt ggcagggcac tttaacttca tgcattcatt ggatagtcta aagcttgcga
     3061 aaaagctgga ccgtttttgt ggtgaggcgg gcagggtgct gccagtttta ttagagttca
     3121 acgtgggcgg cgaagatagt aagggcggtt gggaagccgt tgatgaaacg cgctgggaag
     3181 agttattgcc cgaggtttct gagttgcttg ctatacccaa tttgcaagtc cgcggtttga
     3241 tgtctatgcc gccacttgga atggacgcag agttttcacg cccgtatttc caaaagcttc
     3301 ggcgcttgca agagttcttt acgagccatt tgcctcaagc aacgtggata gagctttcaa
     3361 tgggaacgag ttctgattat gaagttgctg tggaagaagg tgccacgttc gtacggatcg
     3421 gcactgccat tgttggacca agaaactatc aagtagcgga gaatttatga acggcaatac
     3481 tataatatcc atcatcatca tcgttgctga cttctttgta tgggttgtga ttgcatcttc
     3541 gttgctctcg ttttttctac caccctatca ccctgtgcgg gaagcattgg gaaaaattgt
     3601 tgacccgttc ttaaacccga ttcgaagttt gatgcccagt ggtggcgggc ttgattttag
     3661 tccactgatt ttgatcttga tcattgagct aataactaat tttttacgtt ccctattgtg
     3721 aggtgatggt atgtccagag aatatgttct tcatgatggc aagcgcggct cggcgttggc
     3781 agtgcgcata accccgcgag ccagccgcaa tcagattgcc ggagttttaa acgatggcac
     3841 agtcaagatt cagttgtctg ctgatgcgga agataaaaac atgaacagcg acctgcttgc
     3901 gtttcttgcg gaggtgttgg gtgtacctcg cactcgcgta gaaattgtgg caggcg
//
LOCUS       DAOREF010000166         3950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_13846_length_3950_cov_4.300642, whole genome shotgun sequence.
ACCESSION   DAOREF010000166 DAOREF010000000
VERSION     DAOREF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13846_length_3950_cov_4.300642"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="PKI33_04680"
     CDS             <1..1760
                     /locus_tag="PKI33_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNM36330.1"
                     /translation="LLATLTSGPRDMPERQRTLRNMVAWSYNLLTDKEKHMLQAAALF
                     RSGFGIGSLAQVTAVPENEAEHILASLVDKNLIQPFHGDEVRFHLLESIREFALEQAL
                     KQEDVNTLTEKFVGYYLALARDAEQGFRSPEQVQWLDLLDDELANIQTALRFALNDGY
                     AGSFFQSGYEMLIHLQRYWLLRAHYAVGVSWLEKAKAIIDRQVAISSGVISNDLLYQK
                     SKIYGFLGYAYWIMGRYAIAKDLHEISLTAARTLNREDLLWESLNNSAVNLEYMGEFE
                     AAMTYYAQALEIIKRTGDRWQEMRLLINLGNSYVGIEEYETGRTYLEDALLLTMELKD
                     DYYQAACLQGLGYLELRLRRHKEAEENFLKSLSLFERMDVPFIYTWALVSLARVLGET
                     SKHKETAQVVLKCVEMLETQKDRNLYRTMFETLVAFCISLNKNDMAARFIGFIEQNYA
                     GESEVILPADKAIFDAKTQQVGSMMDQREYVLEKETGAKGSASLLFQLASGICDEILN
                     DTHSKPVLLELLTERELSVLRLLSQGRTNEEISKELVVVLKTVEKHVANILRKLGAKN
                     RTEAAIWYLENTSNLRQND"
     gene            complement(1806..2708)
                     /locus_tag="PKI33_04685"
     CDS             complement(1806..2708)
                     /locus_tag="PKI33_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36331.1"
                     /translation="MKRQTLVVILNLALTFSLFGSVSIAHAQTTCTDATGATSPCPPS
                     GGDSGSNEEDDNGSNQPGGGNPNPPTDITATPSPTPSPEPTSTPLPLQAQPTLDPTKN
                     APVFEVTPTPTAEPVITSSGNDGATEGENDWEDVCTFDLCLGLTAACYWDGGTPEIVE
                     DGSGNTGVHCTIPDDDDGAAAPFPWGPVGIVGIVVLLMVMLLPAVQKFKNKPDTPQGR
                     SWDTFGDIKGDNVAPTSTPQTREHILLAKQQDGNTSGASKPKPKPKPAPQPTPPPAQP
                     KPPSSGTPGDFNSDGTVDGNDFIP"
     gene            2907..3470
                     /locus_tag="PKI33_04690"
     CDS             2907..3470
                     /locus_tag="PKI33_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM36332.1"
                     /translation="MEYHEEDVLMRASQGDKDAFGELYERYAERIFNYVYYRTGNQHD
                     AEDLTARVFQRAMNHIRNYTDRGVPFSAWLYRIAHNLVANWHRDRSRKQEIPIDDLPI
                     LPTKGDHPERNLVRSQEQDTLLKMIRKLPPERQNLLILKFVENMSNAEIGKIMGRSEG
                     AVKSLYHRTLLALRDQLEDQNLNLEGE"
     gene            3473..>3950
                     /locus_tag="PKI33_04695"
     CDS             3473..>3950
                     /locus_tag="PKI33_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNM36333.1"
                     /translation="MLRIVTDGAVDLPTEWYKEYDIHRIPINVHFGEEKTFIQDVELD
                     QDGFYKLVDGKTLPHPKTSQPSPHQFAEFYKKIAQPGDTILSIHITSKLSGTYASSVA
                     AAEELKGTYTVVPVDTLCGTMGTAFMCRSARQMERAGKSVDEIKSFIEGVRGKTHVI"
BASE COUNT         1016 a          841 c         1053 g         1040 t
ORIGIN      
        1 cacttttagc cactcttaca tccggtccgc gtgacatgcc tgaacgtcaa cgcaccctgc
       61 ggaacatggt tgcctggagt tacaacctgc tcacagacaa agagaagcac atgctgcaag
      121 cggcggcgct cttccgctcg ggctttggca taggctcact ggcgcaggta acagccgtcc
      181 ctgagaatga agcggagcac atcctcgcct cattggtgga taaaaatctt atccagcctt
      241 tccatggaga tgaggttcgt tttcatctct tggaaagtat ccgcgagttt gcgctggagc
      301 aggctttgaa gcaagaagac gtgaataccc tcacagaaaa gtttgttggg tattaccttg
      361 cattggcgcg agatgccgag cagggttttc gtagccctga acaggtgcaa tggctggatc
      421 tgcttgatga cgaacttgcc aatatccaaa ccgcattgag gtttgcactt aatgatggat
      481 atgcaggttc gttctttcag agtgggtatg aaatgttgat acacttgcag cggtattggc
      541 tgttgcgtgc tcattatgca gtcggtgtat cctggctgga aaaggcgaaa gccatcatcg
      601 ataggcaggt tgccatttca tcgggggtta tctcaaatga tttgctttat cagaaatcga
      661 agatctatgg ctttttgggg tatgcatatt ggatcatggg gcgctacgcg attgcaaaag
      721 atttacatga aatatcactg acggctgcgc gtaccttgaa tagagaagat ctattatggg
      781 aaagtctcaa taactctgct gtcaatctgg aatatatggg tgaatttgaa gcggcgatga
      841 catattatgc acaggcgctt gagatcatta agcggacggg tgatcgttgg caagaaatgc
      901 gtttactcat taatcttgga aattcatacg ttggtattga agaatatgaa actgggcgta
      961 cctatcttga ggatgccctg cttttgacaa tggagttgaa agatgactat tatcaagccg
     1021 cttgtttgca agggcttggt tatcttgaac ttcggttgcg gcgacacaag gaggcggaag
     1081 aaaatttttt gaaatcgcta tccttgttcg aacgcatgga tgtgccgttt atttacacat
     1141 gggcattggt cagtttggcg cgtgttctgg gcgaaaccag caaacacaag gaaaccgcgc
     1201 aggtcgtttt aaaatgtgta gagatgctgg aaacacaaaa agaccggaac ctataccgaa
     1261 ccatgttcga aaccctggtt gctttttgta tttcactgaa taaaaatgac atggctgccc
     1321 gctttattgg gtttattgag cagaattatg cgggggaaag cgaggttatc ctaccggcag
     1381 acaaggcgat ctttgatgca aagacgcaac aggttggcag catgatggat caacgtgagt
     1441 atgtgttgga gaaagaaacc ggcgccaaag gcagcgcttc acttttattc caactagcct
     1501 ctggtatctg tgatgaaatc ttaaacgata cacactcaaa acctgtttta ctggaattac
     1561 tcactgaacg cgagttgagt gtactgcgtt tgctttcgca gggcaggaca aacgaagaga
     1621 tttcaaagga actggtcgtt gtacttaaga ccgttgagaa acatgtggca aatattctca
     1681 gaaagttggg cgccaagaac cgcaccgaag cggcaatttg gtatctggaa aacacaagca
     1741 acctacgtca aaatgactga cgcgggttgc ttgtgtgaaa ttaataggtt tgagattcgc
     1801 aattattaag ggatgaaatc atttccgtct acggtaccat cagaattaaa atctccggga
     1861 gttccactgc tgggtggctt gggttgagca gggggcggag ttggttgtgg cgcgggtttg
     1921 ggcttgggtt tgggtttgga agcgcctgat gtgttgccat cttgttgctt tgccaaaaga
     1981 atatgctcgc gagtttgtgg cgttgaggtg ggtgcaacat tgtcaccctt aatatctccg
     2041 aaagtatccc aagaccttcc ctgcggagta tctggtttat ttttgaactt ttgaactgcg
     2101 ggcaggagca tgaccataag caaaacaaca atcccaacaa taccaaccgg tccccaaggg
     2161 aagggagccg cagcgccatc gtcgtcatct ggaatggtgc aatgaacgcc agtgttgcct
     2221 gacccgtctt ctacgatctc gggtgtgccg ccatcccaat agcaggctgc ggtcaagccc
     2281 aggcaaaggt cgaaggtgca gacatcctcc cagtcatttt ctccctcggt tgcgccatca
     2341 ttgccagagg aagtgattac aggctcggcg gtgggtgtgg gagtcacttc aaagacaggt
     2401 gcatttttag tggggtctag tgttggttgt gcttgaagtg gtaggggcgt actcgtcggc
     2461 tcgggagatg gagtggggct gggtgtagcc gtaatatctg tgggtgggtt ggggtttcca
     2521 ccacctggct ggttgctgcc attgtcgtcc tcttcattag agccgctatc accgccagag
     2581 ggcgggcagg ggctggtggc tccggtggca tcggtacagg tggtttgggc atgggcaatg
     2641 gatacagacc cgaacaaaga gaaggtcagc gccagattca aaattacaac taacgtttgt
     2701 cttttcataa aaagctcctt tgcattggtt ttaacaataa gtgtttgcat tatccccaaa
     2761 gcggttctgc ttgtcggtag ggttaaccct actcttcccc ctgttttttg ataggaaatt
     2821 ggtcatgtta tactgagtgc aacttctcaa atccggcggt gttttattat tcaagttccg
     2881 ccggtaaggt ttctcacttg tcggatatgg aatatcacga agaagatgtg ttaatgcgcg
     2941 cctcacaagg cgacaaggat gcatttggcg agctgtatga acgctatgca gagcgtatct
     3001 tcaactatgt gtactatcgc acggggaacc aacacgatgc agaagattta acagcacgtg
     3061 tatttcagcg ggcgatgaat cacattcgga attacaccga tcggggtgtg ccattttcgg
     3121 cgtggttata tcgtattgcg cacaatctgg ttgccaattg gcaccgtgac cgcagccgaa
     3181 aacaggaaat ccccatcgat gacttgccta tcctgccaac gaagggtgac caccctgaga
     3241 ggaatttagt ccgctcgcag gagcaggata cactactaaa aatgatccgc aaattaccgc
     3301 ccgaaaggca gaatttgctt attttgaaat ttgttgaaaa tatgtccaac gctgagatcg
     3361 gcaagattat gggtagaagc gaaggagccg tgaagagcct gtaccaccgc actcttctgg
     3421 cgcttcgaga ccaactggaa gatcaaaatc ttaatcttga aggagagtaa gcatgttaag
     3481 aattgtgaca gacggcgctg tggaccttcc aacggagtgg tacaaggaat acgatattca
     3541 tcgcattccc attaatgtac attttggtga agagaaaacc ttcattcaag atgttgaatt
     3601 ggatcaagat gggttttata agttagtaga tggaaaaacc cttcctcatc caaaaacttc
     3661 gcagccttcg cctcaccagt ttgcggagtt ctataagaag attgctcaac ctggcgatac
     3721 gatcctttcc attcatatca ccagcaagct ctcagggaca tatgcttcca gtgtggctgc
     3781 cgctgaggaa ttaaaaggaa cctacacagt tgtaccagtc gataccttgt gtggaacgat
     3841 gggcactgcc tttatgtgcc gttctgcccg ccaaatggag cgtgctggca agagtgtaga
     3901 tgaaataaag agttttattg aaggtgtgcg tggtaagacc cacgtcattc
//
LOCUS       DAOREF010000167         3922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14007_length_3922_cov_4.863201, whole genome shotgun sequence.
ACCESSION   DAOREF010000167 DAOREF010000000
VERSION     DAOREF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14007_length_3922_cov_4.863201"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKI33_04700"
     CDS             complement(<1..714)
                     /locus_tag="PKI33_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HNM36334.1"
                     /translation="MSTKNKVSTEQSSISPELASTIPSNLPVLRTKLIGREAEQKTVL
                     DLLNQTDIRLVTLVGFGGAGKTTLALHTSHALFEKFPGGLFFIDLTSIYEPALILPTI
                     AATLGLQEEPSREIADTLRDFLSNRSILFVLDNFEQLISGANIIASFLDANPHIRLLV
                     TSREALHLRGEHTLSLAPLESADAMQVFTQYAQTLNPHFRLTEDNTPAITELCNKLDG
                     LPLAIELAAMRTRMFTPQAL"
     gene            918..3794
                     /gene="uvrA"
                     /locus_tag="PKI33_04705"
     CDS             918..3794
                     /gene="uvrA"
                     /locus_tag="PKI33_04705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNM36335.1"
                     /translation="MSSNKIRVVGARVHNLKNITVEIPRDKFVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFIGQMDKPDVDYIDGLSPAVSIDQKSTSHNPRSTVGT
                     VTEIYDYMRLLFARAGIPHCPVCGREVSKQSAQEIVDKIANLAEGTRILILAPLVRGR
                     KGTYQAVFEEIRKAGFTRARVDGNVYSLDDEIELDRYKQHTIEAVVDRLVISHEGSKE
                     DKKAALTRLTDSIETALKFGEGYITVNLVDKKEDVAYSEHLACPEHGSTVPEIEPRTF
                     SFNTPQGACPDCQGLGSKLEIDPVRIIPDDSVSLNDGAIASMEWGPREEGGYYWSTLV
                     TAAKHYKIDLDAPFRDLTKEQQHVILYGTGDKQLPMKYTNSSGFEVKFSRAFEGVITN
                     LERRYKETNSEYIREKISEYMSDRPCPSCGGKRLNPAALAVTVDNVNIVEANSWPVLQ
                     TLEWVKKISGKGSPLTNKQKTIAERVIREIHERLNFLVNVGLDYLTLNRSAVTLSGGE
                     AQRIRLATQVGSRLVGVLYVLDEPSIGLHPRDNTRLLETLKGLRDLGNTVLVVEHDDE
                     TILESDWVIDLGPAAGEHGGQIIAEGTPKQILAHPKSLTGLYLSGRKQVEIPKKRREG
                     NGKLLKIVNATANNLKGIDVNIPLGKLVCITGVSGSGKSTLMSDILYNELAAKLMGAR
                     TQSGEHKTIEGIEHLDKIINIDQSPIGRTPRSNPGTYTGLFDEIRKLFAELPESKVRG
                     YKPGRFSFNVHGGRCEACQGQGELRIEMQFLPDVYVPCDVCHGKRFNRETLQVMFRDT
                     YSIADVLDMTVDHALEEFKNYPQMQNKLKLLQEVGLGYVRVGQPATTLSGGEAQRVKL
                     SKELSRRATGRTLYVLDEPSVGLHAADVHKLIEVMQRLVDTGNSVLIIEHNLDIIKVA
                     DWLIDLGPEGGNRGGELIGEGTPEQVMKIKGSYTGKYLKEHVNR"
BASE COUNT         1059 a          929 c          984 g          950 t
ORIGIN      
        1 cagggcttgc ggggtgaaca tccgcgtgcg catggcggca agttcgatgg caagcggaag
       61 cccatccagt ttgttacaaa gttcggtaat ggcaggggtg ttatcttcgg ttaatcgaaa
      121 atgcgggttg agggtttgtg catattgagt gaagacctgc atggcatctg ccgattcgag
      181 cggagcaagc gacagggtgt gctcaccgcg caagtgcaac gcctcacggc tggtgacgag
      241 caaacgaatg tgcgggttgg catcgagaaa actggcaatg atatttgcgc cgctaatgag
      301 ctgttcaaaa ttatccaaca caaaaaggat agaacgattg gacaggaaat cgcgcagggt
      361 gtcggcgatc tcgcggctgg gctcttcttg caaacccagt gttgcggcaa tggtcggcaa
      421 aatcaaagcg ggttcgtaaa tggaagtcag gtcaataaag aacagcccgc cgggaaattt
      481 ctcaaacaga gcatgtgagg tgtgcaatgc caacgtggtt ttgcccgcac cgccaaaccc
      541 aacaagggtc acaagacgta tatcggtttg gtttaggaga tccaagacag tcttctgctc
      601 tgcttcacgt ccgatcaact ttgtgcgcaa gacaggcaag ttactaggga tcgtagaagc
      661 aagctcaggc gagatgcttg attgctctgt cgatactttg ttttttgtag acatggtcag
      721 taaaaaatta taacgggttg gtctcttttt tatggcacaa cttactcccc aaaaagtatg
      781 attttgtaag acaccacaat ttacatgaaa tgagaaggcg tgttcaccct gattggtata
      841 atcatttttt ctttttgcca gggactacga ttaccagaac acctgtgcta taataccttc
      901 tcccaaacag gagtctcatg tcatccaata aaatccgcgt cgtgggcgca cgcgtccata
      961 atttgaaaaa tatcaccgtg gaaattccac gcgataagtt cgtcgtcatc actgggcttt
     1021 cgggttcggg caaatcgtcg ctggcgtttg ataccatctt cgccgaaggt cagaggcgtt
     1081 atgtcgagtc gctttcggca tatgcacgcc agttcatcgg tcagatggac aagcccgatg
     1141 tcgattatat cgatgggctc tcccctgccg tttccatcga ccagaaatcc accagccaca
     1201 acccgcgttc taccgttggc acagtgaccg agatatacga ttacatgcgt ctcttgtttg
     1261 cgcgcgcggg cattccgcac tgccccgtct gcggacgcga agtgagcaaa cagtcggcac
     1321 aagaaattgt ggataagatc gctaatctgg ctgaaggtac acgcatcctc atccttgctc
     1381 cactagtgcg cggacgtaaa ggtacatacc aagccgtctt tgaagaaatc cgtaaagcag
     1441 gttttacccg tgcccgcgtc gatggaaacg tctactcgct ggatgatgag atcgaacttg
     1501 accgttacaa acagcacacc attgaagccg tagtagatcg tctcgtcatt tcgcatgaag
     1561 gttcaaaaga ggataagaaa gctgcgctca cccgtctaac agactcgatt gaaacagctc
     1621 tcaaatttgg tgaaggatat atcaccgtca accttgtcga taaaaaagaa gatgtcgcct
     1681 actcggagca tctcgcctgc ccagaacacg gctcaaccgt gcccgaaatt gagccgcgta
     1741 ccttttcctt taacacgcct caaggcgcct gccctgactg ccaaggactt ggttccaagt
     1801 tggaaattga tcccgttcgc atcatccctg atgatagtgt gtcactgaac gatggagcaa
     1861 ttgccagtat ggagtggggt ccgcgtgagg aaggcggtta ctactggagc acacttgtca
     1921 ccgccgctaa gcattacaag attgatcttg atgctccatt tagagatctg acaaaagaac
     1981 aacaacatgt cattctctac ggcacaggcg acaaacaact tccaatgaaa tacaccaact
     2041 cgagcggatt tgaagtcaag ttcagccgtg cgtttgaagg ggttatcacc aacctcgaac
     2101 gccgttacaa agagaccaat tcggaataca tccgcgaaaa aatctcagaa tacatgtctg
     2161 accgtccatg cccatcgtgc ggaggcaaac gtctcaaccc cgctgctctt gccgtgactg
     2221 tagataatgt gaacattgtg gaagcgaatt catggcctgt gctacaaacg cttgaatggg
     2281 tcaaaaaaat ttctggcaaa ggctcaccac tcaccaataa acaaaagacc attgccgaac
     2341 gcgtcatccg tgaaattcat gagcgcttga acttcctcgt caacgttggt ttggattatt
     2401 tgaccttgaa ccgctccgct gtgactctct ctggcggaga agcgcaacgc atccgccttg
     2461 caactcaggt tggttctcgt ctcgtaggcg ttctctacgt tttagatgaa ccatccattg
     2521 gtttgcaccc gcgtgataac acccgtctgc ttgaaacgct taaagggtta agggatctgg
     2581 gaaatacggt tcttgtcgtt gaacatgatg atgagaccat tcttgaatca gattgggtca
     2641 tcgacttagg tcctgctgcg ggcgaacacg gtggacaaat tattgcagaa ggcacaccga
     2701 agcaaatttt ggcgcaccca aagtcactga ctggactcta tctctcgggg cgcaaacagg
     2761 tcgagatccc gaaaaagagg cgcgaaggca atggcaagtt gctcaaaatt gttaatgcaa
     2821 cagcaaataa tttgaagggc attgatgtca atatccctct tgggaaatta gtttgtatta
     2881 caggcgtctc aggttcgggc aagtccacac tgatgagcga catcctgtat aacgaacttg
     2941 cggcaaaact catgggtgcg cggacacaat ctggcgagca taaaacgatt gaaggaattg
     3001 aacatctcga taagattatt aacatcgacc aatcccctat cggacgcacg ccacgttcga
     3061 atcccggtac atacactggc ttgtttgatg aaattcgtaa gttgtttgca gaacttcctg
     3121 agtctaaagt gcgcgggtac aaaccagggc gtttctcctt taatgttcat ggcgggcgct
     3181 gtgaagcctg tcagggacaa ggtgagttac gcatcgaaat gcaatttttg ccagatgtgt
     3241 atgtgccttg cgatgtgtgt cacggcaaac gcttcaaccg cgagaccttg caggtgatgt
     3301 tccgcgatac ctattcgatt gctgatgtac tcgatatgac ggtagaccat gcactggaag
     3361 aattcaagaa ctacccacaa atgcagaaca agttgaagct cttgcaggaa gtagggttgg
     3421 gatatgtgcg ggtgggtcaa cccgcaacaa cactctcggg gggtgaagca cagcgcgtga
     3481 aactctcgaa agaactttct cgccgggcaa cgggacgtac actttacgta ttggatgaac
     3541 cttctgtcgg tttacatgca gcagacgttc acaagttgat tgaagtgatg cagcggctcg
     3601 ttgacacggg caactcggtg cttatcatcg agcataatct ggacattatc aaagtggcag
     3661 attggctcat tgaccttgga cctgaaggcg gtaaccgagg cggggaactc attggagaag
     3721 gtacacctga acaggtaatg aaaataaaag gttcctacac aggcaagtac ttaaaagaac
     3781 atgtgaaccg atagtgcaac ttaaaaaaac aggctggcaa tgccagcctg ttttttgtgt
     3841 cactttcacg caattgcttt ttttcaggta gaattatttt gcacgtaaat ggttggaaaa
     3901 tacatttaga ccgacgtgca aa
//
LOCUS       DAOREF010000168         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14016_length_3920_cov_4.722380, whole genome shotgun sequence.
ACCESSION   DAOREF010000168 DAOREF010000000
VERSION     DAOREF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14016_length_3920_cov_4.722380"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PKI33_04710"
     CDS             <1..521
                     /locus_tag="PKI33_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNM36336.1"
                     /translation="ANQDEIITQIELLLAKRHDVSLDSADFTVTTQQDIISTQESTTS
                     AFRSLLAWVAGVSLIVGGIGIMNIMLVSVTERTREIGIRQSIGATPEDIRLQFLTEAL
                     LLSIVGGLIGVIVGVGGAYIFGSLSDMRTVISLPSILLAFGSSAIVGAFFGYYPANQA
                     SKLDPIEALRYE"
     gene            646..1194
                     /locus_tag="PKI33_04715"
     CDS             646..1194
                     /locus_tag="PKI33_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36337.1"
                     /translation="MSTITKLAIGTLKLEGTENAITPEQASDLLPLWQVYLSLVDSDT
                     AAQAEIDGLVDQISTTLTDSQNQAIDDMQLSQSDMMSVMQEMGISMGNRPQSASDSEQ
                     SGSSGGFPSGGDGFAPPDGGSMPGGGMPSGDIPSGGNMPTLSEDQIATAQASGGAPGM
                     GSGVPTPLIEAVIEYLQKTASS"
     gene            1224..2663
                     /locus_tag="PKI33_04720"
     CDS             1224..2663
                     /locus_tag="PKI33_04720"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNM36338.1"
                     /translation="MNAIHWFPKIPIKAKVGIAIASLIAISAGGYSVFSTQSAAAESK
                     ETALQTATIGRGDLSISASGTGILATEDEVELSFSTSGTVTKVYVHAGDEVQAGDILA
                     EIDNTQAQADYSSAEQTYMELTSPKAIASSLETLASAEKSYSDAIYQLEYLVSPDVLY
                     WDQNIEKAKQNLESAQSQLSSDPENSQVKQRIQELEAYLQSASELRSEALNDYYNEYV
                     FENFVRRDSEGHEYLALPTETDITLAQFAVDDAQRKLEEAQTVYTALSSGIIPTNTGI
                     TSLEEVLSAKRALDEAKSILDGTTIIAPKAGMIVTVDIQTGDKVDTKTCITLTDLLQP
                     HLEIYLDESDWDKIEVGYPVEVEFDTLPDTLFTGTVDTVDSELSTSNMSSSVHGSVAL
                     DAFFAELDLPLGASASVEVISVDLKNILLVPLDALEESDTGTYTVRVLKNNEVKSQAV
                     DIGIHNELYAEIKSGLNEGDIVVTGTFEE"
     gene            2667..3341
                     /locus_tag="PKI33_04725"
     CDS             2667..3341
                     /locus_tag="PKI33_04725"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM36339.1"
                     /translation="MRHENKLNKNLLIGLILVISLSACAPAATEPSENEGPTATSTFD
                     SAIATAVANAFTAAAAVTTNTPTQTSTPAFTATALPTFTFTLAPATETPQPAPPTVLS
                     DYDFCDNSAYVSDITIKDGTVLMPGETFKKIWRLQNNGTCPWRPEYTVTFVSGYNMDG
                     VTTTINQYVAPGKSAKITVAMKAPTAEGTYTGYWSLTNFDGVNFGEVVYVRVVVSKDE
                     TEEEPE"
     gene            complement(3413..>3920)
                     /locus_tag="PKI33_04730"
     CDS             complement(3413..>3920)
                     /locus_tag="PKI33_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36340.1"
                     /translation="GSLTYTDANSPLFYVNNSTGYITLDHVAVNVASGILVNAAANDR
                     WGTSGQNGGTVYLTATNQTLSGHFIADAISAINLELTDSSSWTGAINANQEASSSSLT
                     LDASSTWTVTADSYLTCLSDPEGISGATIQNIIGNGHTVYYDAANCKALNGITYTLVG
                     GGELKPLQ"
BASE COUNT         1118 a          935 c          885 g          982 t
ORIGIN      
        1 atgccaatca agacgagatc attacccaga tcgaactgtt attagccaaa cggcacgacg
       61 tttctctaga ctcagcagat tttactgtca caacacagca agacatcatt tcaactcaag
      121 agtcaacaac ttcagctttt cggtcacttt tggcttgggt ggcgggcgtt tctctgatcg
      181 ttggcggcat cggcatcatg aacattatgc tggtcagtgt caccgaacgg acgcgtgaga
      241 tcggcattcg gcaatcaatt ggcgccaccc cagaagatat ccgcttgcag tttttgacgg
      301 aagccctttt gctcagcatt gttggcggtc tgatcggagt catcgtaggc gtgggtggag
      361 catacatctt cggctcttta agcgacatgc gaactgttat ctccctcccg tccattctcc
      421 tagcgtttgg ctcttctgcc attgtcggcg catttttcgg atattaccca gccaaccaag
      481 cttcaaaact cgaccccatt gaagcgttgc gatatgaata aaggaaattt tatgaaaaag
      541 atatttgtaa tttcattgat gctatgttca ttagctcttt ctgcttgtag cagttccgca
      601 tcaaccccaa caggtggaat gccttcttct aacgacggag gtgacatgag caccatcacc
      661 aaacttgcta ttggaacact caaactagaa ggaaccgaga atgccatcac gcctgagcaa
      721 gccagtgacc ttttgcctct ttggcaagtt tatctctcac tagtagacag cgataccgcg
      781 gctcaagcag aaatcgacgg cttggttgac cagattagca caacactgac cgattcccaa
      841 aaccaagcga ttgacgacat gcaactttct cagtcagaca tgatgtctgt gatgcaagaa
      901 atgggcattt ccatgggaaa ccgtccacaa agtgctagtg atagcgagca aagcggaagc
      961 tcgggaggct ttcccagtgg tggcgatggg tttgctccac ctgatggtgg cagtatgccc
     1021 ggtggtggca tgcccagcgg agacataccg agcggcggaa atatgccaac cctcagcgaa
     1081 gatcaaattg ccactgcaca agcttctgga ggcgctcccg gcatgggaag tggtgtacca
     1141 actcctttga tcgaagccgt gatcgaatac cttcaaaaaa cagccagttc atagtgtttg
     1201 aaccgccgta gaaatgatat gggatgaacg caatacattg gtttccaaaa attcctataa
     1261 aagctaaagt tggcattgct atcgccagtc ttatcgcaat cagcgcaggt ggatattctg
     1321 tgtttagcac tcaatccgcc gctgctgaaa gcaaagaaac tgcattacag accgctacca
     1381 tcggtcgcgg agacttaagc atttccgcta gtggcactgg aattttagca acagaagatg
     1441 aagtggagtt atcgtttagt accagcggaa cggtcacaaa ggtgtacgtc catgctgggg
     1501 atgaagttca ggcaggcgat atactagccg agattgacaa cactcaagct caagcagatt
     1561 actcaagtgc agagcaaacg tacatggagt taacctcccc aaaagcgatt gcatcttccc
     1621 tagaaacatt ggcaagcgcg gaaaaatcat actcagatgc tatttatcag cttgaatatc
     1681 ttgtcagccc agatgtactg tattgggatc aaaacatcga aaaagcaaag caaaatctgg
     1741 aaagtgccca aagtcaatta tcaagcgacc cagaaaactc tcaagtaaaa cagcgaattc
     1801 aagaattaga agcctactta caatctgcaa gtgagcttag atctgaggct ttaaatgatt
     1861 attacaatga atatgtcttc gaaaattttg tccgccgcga tagcgaaggg cacgaatatt
     1921 tagcattgcc aacagaaaca gatatcactc tcgcgcagtt tgcagttgat gacgcacaac
     1981 gaaagctaga agaagcacaa accgtttaca cagccttatc tagcggaata attcctacta
     2041 atacaggaat tacaagcctt gaagaagtcc tttcggcaaa gcgggcgctt gatgaagcaa
     2101 aatccatttt agatggaaca acgatcatcg ccccaaaagc agggatgatt gttactgtgg
     2161 atattcaaac tggcgacaaa gtggacacaa aaacttgcat cacccttaca gatttattac
     2221 aaccgcactt ggaaatctac ttggatgagt cagattggga taagatagag gtcggttatc
     2281 cggtagaagt cgaattcgac accctgccgg atactctatt taccggcacc gtagacacag
     2341 tagatagcga actgtccacc agcaatatgt cttcttcggt gcatggtagc gtagctctag
     2401 atgccttctt tgcagagcta gatctccctc ttggagcatc cgcttcagtt gaagtcataa
     2461 gcgttgactt gaagaatatt ttgctcgtcc cgcttgatgc gctggaagaa tcggatactg
     2521 gcacatatac tgttcgtgtc ctaaaaaata atgaagtaaa atcgcaagct gttgatattg
     2581 gtattcataa cgagttgtac gccgaaatca aatccggttt aaacgaaggt gatattgtcg
     2641 ttaccggcac attcgaagag tagatcatgc gccatgaaaa caagctaaac aaaaatcttc
     2701 ttatcggtct cattttggtc atcagtttgt ctgcttgtgc gccagcagcc acagagccca
     2761 gtgaaaacga gggtcccaca gccacaagca ccttcgactc agccattgca actgcggttg
     2821 caaacgcttt taccgctgct gctgccgtaa caaccaatac tcctacccaa acatcaacgc
     2881 cggcatttac agccaccgct ttacctactt ttacatttac gcttgcccct gcgacggaaa
     2941 cgcctcaacc cgcgccgcca acggttctta gtgattatga tttttgcgac aactcagcct
     3001 atgtgagtga catcactatc aaagatggga ccgtactcat gcctggggaa actttcaaaa
     3061 agatatggcg tcttcaaaat aacggtacct gcccatggcg cccggaatat acagtcacct
     3121 ttgtcagcgg atacaacatg gacggtgtaa ccaccaccat taaccaatat gtagccccag
     3181 gaaaatcagc caagattaca gttgccatga aagccccaac tgccgaaggg acatataccg
     3241 gatactggtc attgactaat tttgatggcg tcaattttgg tgaagtcgtt tatgtgcgtg
     3301 tggtcgtgtc taaggacgaa acagaagaag aacccgaata acgttattcg tacaatataa
     3361 gaagcgcttc cattctatgg aagcgcttct cttgtttaag agttggaatt acttattgaa
     3421 gtggttttag ttcaccacct ccaacaaggg tataggtaat accattcaaa gctttacagt
     3481 ttgcggcatc ataatatacg gtgtgaccgt tcccaatgat attctgaatg gttgcccccg
     3541 agatgccttc ggggtcactc aaacaagtaa ggtaggagtc agctgtcacg gtccaagtac
     3601 ttgaggcgtc caacgtcagt gaagaagaac tcgcttcctg gtttgcgttg atggctccag
     3661 tccatgacga gctatcagtc aattctaaat tgatggcact aatggcatct gcgataaagt
     3721 gaccgctcag ggtctggttg gtggcagtca aatatactgt tccaccattc tgtccacttg
     3781 tgccccaccg atcatttgca gccgcattca cgaggattcc agatgcaaca tttaccgcaa
     3841 cgtgatcgag agtaatgtat ccagttgagt tgttgacata gaacaaggga ctattagcat
     3901 ctgtatacgt caacgacccg
//
LOCUS       DAOREF010000169         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14078_length_3909_cov_3.580436, whole genome shotgun sequence.
ACCESSION   DAOREF010000169 DAOREF010000000
VERSION     DAOREF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14078_length_3909_cov_3.580436"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKI33_04735"
     CDS             <1..611
                     /locus_tag="PKI33_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM36341.1"
                     /translation="GALNENQMHFLEVVRNNIERLNILVSDLLDISRIESGRVKLEQG
                     KVNLYDIAEEVVADVLRRSQNESKPIALSLDAPKNLPPIQGDGARVRQIISNLVNNAF
                     NYTPENGAILVNIHDADGEVQVDVQDNGIGIKPEDQDRLFERFYRGEHPLVLATPGTG
                     LGLPIVRQLVEMHNGRIWMKSTGVPGEGSIFSFTLPVYKNGA"
     gene            618..992
                     /locus_tag="PKI33_04740"
     CDS             618..992
                     /locus_tag="PKI33_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36342.1"
                     /translation="MARILIAEDEPDIRELVAFTLRFAGHEVTTTANGEEAYHKAQEM
                     IPDLIMMDVRMPKMTGYDACRAMKQIPSLKDIPVVFLSAKGQDSEIQTGLDAGAEEYL
                     LKPFAPDQLVERVKAILAKFGK"
     gene            993..1781
                     /locus_tag="PKI33_04745"
     CDS             993..1781
                     /locus_tag="PKI33_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM36343.1"
                     /translation="MSAIILDGSMVHYEALGRGRPIIFLHGWVGSWRYWINAMQAAST
                     SFRAYAIDLFGFGDTVRSENNYTIEKQALLLERFLNEMGIGKVALVGHDLGALVAFEY
                     LKKHYESVDRMMTIGVPLQYETLATRMRTAPTTELAEWLSARQPDALTALADASKADP
                     LAVTTSMSSLQVDGIFPNVRNFGVPCLFVYGANDPAFTTVPSQETSNLPIHMHQINLE
                     GSGHYPMLDNVSQFNRLLIDFLALDSGASPSELQMKEEWKRRVR"
     regulatory      1854..1963
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2036..2674
                     /locus_tag="PKI33_04750"
     CDS             2036..2674
                     /locus_tag="PKI33_04750"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNM36344.1"
                     /translation="MRIIIFANGNLPNLEKAHSLICPDDFILCADGGTRHALALGLTP
                     NVVIGDLDSVTEEERRKMKEMGVEVMEFSMDKNETDLELTINHALTLEPEQILILAAL
                     GGRMDQTLANISLLSDLRLSTFDVRLTDGVEEIFFCRDQAKVEGRSGDIVSLIPWLGE
                     VTGVFTENLRWHLHHETLYPEKTRGISNEMTSDVATVSITSGLMLITHITGN"
     gene            2700..3734
                     /locus_tag="PKI33_04755"
     CDS             2700..3734
                     /locus_tag="PKI33_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNM36345.1"
                     /translation="MKKIFPILFLLFAFLFAACAPKGPVTLTVMTHDSFAVSEDVVTT
                     FEQANNADVVFLQSGDAGTMLNKAILAKDAPLADVMFGVDNTFLSRALEADIFDAYQS
                     PALSDVSAEFVLDASYRALPVDYGDVCINYDKKYFEENNLTLPASFEDLAKPEYKGLL
                     VVENPATSSPGLAFMLATRAYFGDSFLDYWQSLKDNGVVVVDGWETAYYTNFSASSGQ
                     GPQPMVVSYASSPAAEVFFATEPPADAPTASIVASGMCFRQIEFVGILKNGQNRDLAE
                     KFVDFMLGKQFQEDMPLNMFVYPVNQSAQLPDVFMQYAQVAKQPASITYDDIAANRDA
                     WIEAWNEVMK"
BASE COUNT         1002 a          994 c          904 g         1009 t
ORIGIN      
        1 cgggtgcttt gaatgaaaac cagatgcact tcctcgaagt ggttagaaat aacattgaaa
       61 gacttaacat tctcgtgagc gatttgcttg atatttcccg catcgaatct ggacgtgtga
      121 aacttgagca aggcaaggtc aatctgtatg atattgctga agaagtggtg gcagatgttt
      181 tacgccgctc acaaaatgaa agcaagccga ttgcactttc actagatgcg ccgaagaacc
      241 ttccacctat tcaaggcgat ggcgcaagag tccgccagat cattagcaac cttgtaaaca
      301 atgcattcaa ttacaccccc gaaaatggcg ccattctggt gaatatccat gatgctgatg
      361 gcgaagtgca agtagacgtg caggataacg gcattggcat aaagcctgaa gaccaagacc
      421 gcctctttga acgtttttac cgtggcgagc atccacttgt gttggcaacg ccgggcactg
      481 gtttaggttt gcccatcgtt cgtcagttgg tcgaaatgca caatggacgt atttggatga
      541 aaagcacagg cgtccctggt gaaggaagta ttttctcttt caccttgcct gtctataaaa
      601 acggagcata aatttcaatg gcaagaattt tgatcgcaga agatgaaccg gatattcgtg
      661 agttggtagc gtttacgtta cgctttgcgg gtcatgaagt gaccaccact gccaacggcg
      721 aagaagccta tcacaaagca caggagatga ttccagacct catcatgatg gatgttcgta
      781 tgccgaaaat gacgggctat gatgcctgcc gtgccatgaa gcagattcca tctttgaaag
      841 atatccctgt ggtatttctc tctgcaaaag gacaggactc tgaaattcaa accgggcttg
      901 atgcaggagc cgaagaatac ctgcttaaac cttttgcccc agaccaactt gtggaacggg
      961 tgaaagcgat ccttgcgaaa tttggaaaat aaatgagcgc aattattctt gatggttcga
     1021 tggtgcatta cgaggcgttg ggacgtggtc gtcccatcat cttcctgcac ggctgggttg
     1081 gctcatggcg atattggatc aatgccatgc aagccgcatc cacatcgttc cgcgcctatg
     1141 ccatcgatct ctttggtttt ggcgataccg ttcgcagtga gaataattac accattgaaa
     1201 aacaagctct gttgcttgaa cgcttcttga atgaaatggg tattgggaag gtcgctttgg
     1261 tagggcatga ccttggcgcg ctcgtcgctt ttgaatacct taaaaagcac tacgagagtg
     1321 tagaccgtat gatgacaatc ggcgtaccgt tgcaatacga aacacttgcc acacgcatgc
     1381 ggactgcccc cacaaccgaa cttgcggaat ggctgtctgc gcgccagcct gatgccttga
     1441 ccgctctcgc agacgcctcc aaagcggacc cgttggctgt gaccacctcg atgagcagtt
     1501 tacaagttga cggtattttc ccgaacgttc gtaattttgg tgtgccatgt ctttttgtat
     1561 atggtgccaa tgacccagcc ttcacgaccg tcccttcaca agagacaagt aatctaccca
     1621 ttcatatgca ccagattaac ttggaaggat ctgggcatta ccctatgctt gataacgtct
     1681 cgcaattcaa ccgcctcctg atcgatttcc tcgcactcga ctcaggcgcc agcccaagtg
     1741 aactgcaaat gaaagaggaa tggaaacgcc gcgttcgcta aaaataccgt gagaaaaaaa
     1801 tctcgcggta tttttttact tgacattcct acgtcacagt cctatactcc aaacacaacc
     1861 gaacggagag cctttacgaa aggctgagag gagcgtgaac gcgctcgatc cgcattacct
     1921 gatctggtta ataccagcgg agggatttct caatacacct gccaacatcc tccgctgctg
     1981 tgaggatgtt tttgttttaa cctttgtgta ccttcgtgac cctttgtggt gaaaaatgag
     2041 aatcatcatc tttgccaacg gcaacctgcc aaacctcgaa aaagcccact ccctcatctg
     2101 ccccgatgac ttcattcttt gcgcggacgg tgggacacgt catgcccttg cgttgggact
     2161 cactcctaat gtggtgattg gagatctgga ttcggttacg gaggaagaaa gaagaaagat
     2221 gaaagaaatg ggcgttgaag ttatggaatt ttcgatggat aagaacgaaa cagacctcga
     2281 actcaccatc aaccatgcac tgactcttga gcctgaacaa atcctaatcc tcgccgccct
     2341 cggcggtcgc atggaccaaa ccttagccaa catatccctt ctctctgacc ttcgactttc
     2401 gaccttcgac gttcgactca ccgacggcgt agaagaaatt ttcttttgca gagaccaagc
     2461 caaagttgaa ggaagaagcg gagacatcgt ctcgctcatc ccctggctgg gcgaggtcac
     2521 cggcgtcttc accgaaaacc tacgctggca tcttcatcat gaaacgcttt accccgagaa
     2581 gacccgcggc atcagcaacg aaatgacatc cgatgttgcc acggtttcaa ttacgagcgg
     2641 attaatgctc atcactcaca tcactggtaa ctgattactg gttactaaaa ggaacaccca
     2701 tgaaaaaaat atttccaatt ctctttcttc ttttcgcttt ccttttcgca gcttgcgccc
     2761 cgaaaggacc tgtcaccctc actgtcatga cccatgactc attcgccgtt agcgaagatg
     2821 ttgtgaccac ctttgaacaa gccaataatg ccgatgtcgt cttcctgcaa agcggtgacg
     2881 caggcaccat gctcaacaaa gccatcctcg ccaaagacgc cccgcttgcg gacgtaatgt
     2941 tcggcgtgga caacaccttc ctttcgcgtg ccctcgaagc ggatatcttc gatgcgtatc
     3001 aatcgcctgc attgagtgat gtgtccgccg agtttgtgct tgatgcttct tatcgtgctt
     3061 tgccagttga ttacggtgat gtctgcatca attacgataa aaaatatttt gaagaaaata
     3121 atttaacttt gcccgcttcc tttgaagatt tggcgaagcc tgaatataaa ggtctgctcg
     3181 tggtggaaaa ccccgccacc tcttcacccg gtttggcgtt catgcttgcc actcgtgcgt
     3241 attttggtga cagcttcctt gattactggc agagcctcaa agacaatggg gtggtggttg
     3301 tggatggttg ggagactgcc tactacacta acttctcagc ttcttctggg cagggaccgc
     3361 agccaatggt cgtctcctac gcctccagcc ccgccgcgga agtgttcttt gccaccgaac
     3421 cgcctgctga tgcgccgact gcttccatcg tcgcttcggg catgtgcttc cgccagatcg
     3481 aattcgtggg catactcaag aacggacaga accgcgacct cgccgaaaaa tttgtagact
     3541 tcatgctggg caaacaattc caagaagaca tgccgctcaa tatgttcgtc tatcctgtga
     3601 atcaatccgc acaactgccg gatgtgttca tgcaatatgc tcaagtcgct aagcagcctg
     3661 catccattac ttatgatgat atcgccgcca accgcgacgc atggatcgaa gcatggaatg
     3721 aagtgatgaa ataaaaatat cattcaccac agatgaacgt ggataaaaag aaatccaatt
     3781 catctgcgtt tatctgtggt tgaaatttgc aaatgagtct ctcactaacg cttgctaaaa
     3841 aaactcgttc gtttttatct gccaattcgc ggattttgct ttggattccg actcttctct
     3901 ttttgcttt
//
LOCUS       DAOREF010000170         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14216_length_3886_cov_4.146959, whole genome shotgun sequence.
ACCESSION   DAOREF010000170 DAOREF010000000
VERSION     DAOREF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14216_length_3886_cov_4.146959"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKI33_04760"
     CDS             <1..236
                     /locus_tag="PKI33_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36346.1"
                     /translation="GTGNEAPIKLDHLKDFYPWLDETGFQNKWIRTDYKFESLEEAEE
                     LSRFFFGDELGNKVVQNSWTILPECTGVWWLKM"
     gene            complement(278..838)
                     /locus_tag="PKI33_04765"
     CDS             complement(278..838)
                     /locus_tag="PKI33_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36347.1"
                     /translation="MLQNLSTKFYGWAKGWLVLILLVLDGFFGGFLLPLIQGLMQGGS
                     GGIVPLDLMLFSTPDKLFAMIEKYGDSTRIFYRNVELTVDIIYPIIYLFFFGLAISWL
                     FQRGFAPNSPMRKFNVVPLGAWFVDLLENIVIVSLISIFPAKPMALGWVLFLLTTVKW
                     LFAGASILLILVGLVMAIKNGFKKQE"
     gene            complement(957..1421)
                     /locus_tag="PKI33_04770"
     CDS             complement(957..1421)
                     /locus_tag="PKI33_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNM36348.1"
                     /translation="MSNILYGPRLGKEGELRVGCSATLFDETRQKVLLTQRMDNGRWC
                     LPGGHMESGESAAEACEREVWEETGLRVRAIKLLGVYSNPDQLVIYKDGSKAFFVVLN
                     FEVEILEGTLGLSNETTDYGYFTLEEMGTMPMHGEHKLRVEDAMRGGDAVMR"
     gene            complement(1471..2310)
                     /locus_tag="PKI33_04775"
     CDS             complement(1471..2310)
                     /locus_tag="PKI33_04775"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNM36349.1"
                     /translation="MTNRTISQIIEPNMVIEGAGVLLRRSFGPHASNKFDPFLLFDHF
                     AFNNPLEGPSRGFPTHPHRGIETVTYMLEGTVRHRDSLGNQGTIAEGDVQWMTSGRGI
                     LHEEMPRRSANGNIYGFQLWVNLPAAQKMGEPRYQEVAAGTIPVVEKDGVQIRLVAGE
                     VDGIRGPVTEIAAAPLYMDVKLAPGSQFVHPIPSGHTTLMYVFEGVGEFSGEVVEAVS
                     MIKFNDDGDQVEVKTEAGVRFMLIAGAPFKEPIFPYGPFVMNTKEEIEETLRELRNGT
                     FIK"
     gene            complement(2372..2740)
                     /locus_tag="PKI33_04780"
     CDS             complement(2372..2740)
                     /locus_tag="PKI33_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNM36350.1"
                     /translation="MSPIRMAKIESGMRVVLDFNEAFNRHDVSKIADLLRADCVFETT
                     NPAPDGSIYKGKEAIIEYLKSFFQRSPQAHMEVEEVFGMGLHCILRWKYTWGDGYVRG
                     VDIFKVEEGLICEKFSYVKG"
     gene            complement(2737..3222)
                     /locus_tag="PKI33_04785"
     CDS             complement(2737..3222)
                     /locus_tag="PKI33_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36351.1"
                     /translation="MKLQIEPIDPLHEEAIYLLHEAAREIRPLYNSGQDPNLNLPTNE
                     PLGGRSAFLIARVDGKPVGCAALRPIEGDIAELRRMYVLISYRGQGIAQKLMEEIEKV
                     AIEFKYIMIRLETGNRQLRAIALYKACGYQRIEPFGPYVGDPTSVCFEKQLSGPERGD
                     V"
     gene            complement(3294..>3886)
                     /locus_tag="PKI33_04790"
     CDS             complement(3294..>3886)
                     /locus_tag="PKI33_04790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNM36352.1"
                     /translation="TLSILESILVMAFVRPWPFMTQYIVLFSAVFFGINLILDFHQLY
                     QENIELQVKANIDPLTGVFNRNALKDSLVRQYNTLALIDLDNFKYFNDKHGHAEGDKL
                     LLLFVDNLQKNIRQDDRIVRIGGDEFILLLNHAVPHQAYETLERICTQFAKVVPDSHV
                     SLSYGIESITDSLEKSISSADMRMYEMKTEHKQVKR"
BASE COUNT         1046 a          893 c          862 g         1085 t
ORIGIN      
        1 tgggaactgg gaacgaggct ccaatcaagt tggatcacct caaagatttt tatccgtggt
       61 tagatgaaac aggtttccaa aataaatgga ttcgcacaga ctacaaattt gaatcattgg
      121 aggaagccga agaactttcc cgctttttct ttggcgatga gttgggaaat aaagttgtgc
      181 aaaatagttg gactatcttg ccagaatgca ctggtgtatg gtggttgaag atgtaaatag
      241 atggcagtca cttctaaagt gactgccatc tattttttta ctcttgtttc ttgaacccgt
      301 tcttgatagc cattaccaag ccgaccaaaa tcaacaaaat gcttgcgcct gcaaacagcc
      361 atttgacagt ggtgagaagg aacaacaccc atcccaacgc cattggcttt gcagggaaaa
      421 tggaaatcaa agacacgatc acgatgtttt ccagaaggtc cacaaaccag gcgcctaatg
      481 gcacgacatt aaacttgcgc atggggctat tgggggcaaa cccacgttgg aaaagccaag
      541 agatcgccaa gccaaagaag aataaataaa tgatggggta gataatatct acggttaatt
      601 ccacgttgcg gtaaaaaatg cgtgtagagt caccgtattt ttcgatcata gcaaaaagct
      661 tatcaggcgt ggaaaaaagc atcaggtcga gcggaacgat gccgccactg ccgccttgca
      721 tcaacccttg aatgagcggc aaaagaaacc cgccaaagaa cccatccaat actagtaaaa
      781 tgagaaccag ccagcctttt gcccagccat aaaacttggt agaaagattt tgtaacatac
      841 tgcctccatg aataaaaata agcgaccatc atcgaataat gattgccgct tatattaacc
      901 catgaaattg ttctaggctt ggttttcagt acttttgtag gggcaacgcg cgaaaattag
      961 cgcatcactg catctccgcc gcgcatggcg tcttctacgc gtaatttatg ttctccatgc
     1021 attggcatcg tgcccatttc ttctagagtg aagtagccat aatctgtggt ctcattgctt
     1081 aatcctaaag ttccttcaag aatttccact tcgaaattaa gcaccacaaa gaacgcctta
     1141 cttccatctt tataaataac taattgatcg gggttgctat atacacctag taattttatg
     1201 gcgcgaactc ttaagccggt ctcttcccaa acttcgcgtt cgcaggcttc tgccgcactt
     1261 tcgcctgatt ccatatgccc gccgggcagg caccaacgtc cgttgtccat tcgttgggtg
     1321 agtaaaactt tctggcgagt ttcgtcgaag agggtggcac tgcacccaac ccgaagttca
     1381 ccttcttttc cgagccgagg tccatagagt atgtttgaca ttccctaatt ttaatgcgaa
     1441 cggcggattt gatcatccgc cgtctgttcg ttatttgatg aatgtgccgt tgcgtagttc
     1501 tcgcaatgtt tcttctattt cttcttttgt gttcattacg aatggaccat atgggaatat
     1561 gggctctttg aatggggcac cggcaatgag cataaagcgg acgcccgcct ctgtcttaac
     1621 ttcaacctgg tcgccatcat cgttgaattt gatcatgctg acagcttcta caacctcacc
     1681 cgaaaactca cctacgcctt caaaaacgta catcaatgtg gtatgtccgc ttgggatggg
     1741 gtgaacgaat tgggaaccgg gtgccagctt cacatccatg taaagaggcg cagcggcaat
     1801 ctcggtcact ggtccacgga tgccatccac ttcgccagcc acgagtcgaa tctgcacacc
     1861 atccttctct acaacgggaa tggttccagc ggcaacttct tgataacgcg gttcgcccat
     1921 tttttgagca gcaggcaagt tcacccaaag ttggaagccg tagatatttc cgttcgcact
     1981 gcggcggggc atttcttcat gcaagatgcc gcgcccgctg gtcatccatt gcacatcgcc
     2041 ttcagcgatg gtaccttgat taccgaggct atcccgatgg cgcactgtgc cttcaagcat
     2101 atatgtaacg gtttcaatgc cgcggtgagg gtgggtggga aaaccacgac taggaccttc
     2161 tagtggattg ttgaatgcga agtggtcgaa gagtaagaac gggtcaaatt tattgctggc
     2221 atgtggaccg aaagaacggc gcagtaaaac gccggcgcct tcgatcacca tattgggttc
     2281 gataatttgg gaaatggttc ggttggtcat gaaatgtaga tgatttgaaa aagatgaacc
     2341 ttacgaataa acattatttc acactacacg tttagccttt tacgtacgaa aacttttcgc
     2401 agatcaaccc ttcttcaacc ttgaaaatat ccaccccgcg cacataacca tcaccccaag
     2461 tatatttcca gcgcaagatg caatgcaacc ccataccaaa aacttcttca acttccatat
     2521 gagcttgcgg cgacctctga aagaagcttt ttagatattc gatgatagct tctttgcctt
     2581 tgtagatact cccatctgga gctgggttgg ttgtttcaaa gacgcaatct gctctcaaaa
     2641 ggtctgcaat tttggataca tcatgccggt tgaatgcctc attgaagtca aggacaacgc
     2701 gcattcccga ctcgatcttt gccattcgaa taggactcat acatcgcctc tttctggacc
     2761 tgaaagttgt ttttcaaaac atacactggt tgggtcgccg acatagggtc cgaacggttc
     2821 gattcgctga tatccacatg ctttatacaa cgcaatggct cgtagctgcc ggttgccggt
     2881 ctcaagacga atcataatgt acttaaactc gatagctact ttttcgattt cttccattaa
     2941 cttttgcgcg atgccttgtc cgcgatatga gatgagcaca tacattcgtc tcaattctgc
     3001 tatatctcct tctatggggc gcaatgcggc acaaccgaca ggcttgccat ctacacgggc
     3061 aattaaaaac gcactacgcc ctcccagcgg ctcgttcgtt gggaggttga ggtttgggtc
     3121 ttgtccacta ttgtataaag gacgaatttc tcgcgccgct tcgtgaagca ggtagatggc
     3181 ttcttcatgt agcgggtcaa tgggttcgat ttggagtttc atggttgtgt attgacttgt
     3241 tgttttgtaa atctataaat atgttatacg aagtaatctc gtccttctat gttttacctt
     3301 ttaacctgct tatgctcagt tttcatttca tacatgcgca tatccgcact gctgatgctt
     3361 ttctccaacg agtctgtaat agattcgatg ccataagaca aactgacatg cgaatctggc
     3421 accactttcg cgaattgggt gcagattcgc tctagggttt cgtatgcttg atggggtacg
     3481 gcatgattta gtaggagaat aaattcatcg ccgccaatac gaacaatgcg atcgtcttgt
     3541 cggatattct tttgtaaatt gtctacgaac aataaaagga gtttatctcc ttcggcgtga
     3601 ccatgcttgt cattaaagta tttgaaatta tcgaggtcta ttaatgctaa tgtgttgtat
     3661 tgacgcacta aagagtcttt tagggcattt cggttaaata caccggtgag agggtctata
     3721 ttggctttga cttgaagctc aatattttct tgataaagct ggtgaaaatc aagaattaaa
     3781 ttgataccaa aaaagacagc gctgaaaaga acgatgtatt gggtcataaa gggccatggt
     3841 ctgacaaatg ccataaccaa aatagactca agaatggaaa gagtta
//
LOCUS       DAOREF010000171         3871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14303_length_3871_cov_5.390199, whole genome shotgun sequence.
ACCESSION   DAOREF010000171 DAOREF010000000
VERSION     DAOREF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14303_length_3871_cov_5.390199"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKI33_04795"
     CDS             <1..847
                     /locus_tag="PKI33_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNM36353.1"
                     /translation="AGLQATQENGLADQVDYIASVDARDYQKNITYFADLGYDVIFTS
                     GISLEDETLYSADLTPDSVFVGLNQTFEEPHSNLISVTFPEDQMGFAAGVGAASLTKT
                     GIVAAVCETSGIDAMWRYCEGFRAGVKHVSPELQALVVYREDGDREKLFIDEQWGYDT
                     AKNLIERGADVVFAAGGVTGQGALKAATELHVYAIGSERDQAAVLGVQGSSVVTSVYG
                     QSQLTVQDVMRMLREGKLSKTWQGSVGYIPLSSAYPPEVSLGLDLLLGALKRGEIQTN
                     VTKSH"
     gene            956..2092
                     /locus_tag="PKI33_04800"
     CDS             956..2092
                     /locus_tag="PKI33_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNM36354.1"
                     /translation="MKKLYFVMAALIIASMVLAACGTPAATEAPAATEAAATEAAATE
                     APAATEAAAATIKVGEVTDLGGIDDKSFNANGWAGAQKAAAELGVEAKYLESTQQADY
                     AKNIQQFVDEGTNLIVTVGFLLGVDTATAAKANPDTHFAIVDYAYPDCFGTQVEGKDC
                     GSATELPNVLGLTFQIDQAGFLAGYAAAASTETGKVATFGGINIPPVASFMIGFEKGV
                     LYYNEQNGTAVEVLGWNTEANDGSFTGNFDSLDDGRSFAESFVQEGADIIMPVAGPVG
                     LGSAAYCQESGACKIIGVDVDWTVSAPEYKDIILTSVLKNINVAVYDAIQAEVDGTFA
                     GGVYVGTVENGGVGISDVAGASDDLKAQLDAVTQGIIDGSIATR"
     gene            2283..3815
                     /locus_tag="PKI33_04805"
     CDS             2283..3815
                     /locus_tag="PKI33_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36355.1"
                     /translation="MTNVLELRGITKRFPGVLANDKISITLREGKILALLGENGAGKS
                     TLMNILYGLYKPDEGQIIVRGKEVDIQGPNDAIAQGIGMVHQHFMLVPVMTVTENVML
                     GIEPTKNGIFLDKEKVANRIREISDEYGLEVDPNAYIKDLPVGIQQRVEIIKVLYRHA
                     DILILDEPTAVLTPQEVEGLFKIIDTLIKAGKSIIFITHKLKEVLAVADDITVLRLGT
                     VVGSIDPKEATSEILATMMVGRDVTLVVQKKLSEPGRAVLDVKDLYVRDERQNMTVQG
                     VSFEVRKGEVLGVAGVQGNGQTELVYALTGLLPLEAGTIHLLDEAIHHTSPRHILEKG
                     VAHIPEDRQRHGLILSFPIHDNMMLCTYYKDPFAKGISLQQKTIFENADQLVKQYDVR
                     TPNIYVNAGTLSGGNQQKVIVAREFSRPIELLIASQPTRGLDVGSIEYIHSQIIKKRD
                     EGSGVLLVSSELDEILALSDRIAVMYKGQIMAIVDASKATKEQLGLLMAGVHPSEPSN
                     KN"
BASE COUNT          944 a          936 c         1005 g          986 t
ORIGIN      
        1 ggcggggtta caagcaacgc aagaaaatgg tttggcagat caagtggatt acattgcttc
       61 tgtcgatgcg cgtgattatc aaaagaacat cacttatttt gcagatcttg gctatgacgt
      121 catttttaca tctggcattt ctttggagga cgaaacgctt tatagtgcgg acctcacgcc
      181 tgattctgtt ttcgtgggct tgaatcagac ctttgaagaa ccccactcca acctaatctc
      241 ggtcactttc cctgaagacc aaatgggctt tgccgccggg gttggtgccg catcgttgac
      301 caaaacgggc atcgttgcgg cagtatgtga aacctctggc attgatgcca tgtggcgcta
      361 ttgcgaaggc tttcgggctg gcgtgaaaca cgtcagccca gagttgcagg ctttggtggt
      421 gtaccgtgaa gatggagata gagaaaaact gttcattgat gaacagtggg ggtatgacac
      481 ggcgaaaaat ctaattgaac gcggagccga tgtggtcttt gccgcgggcg gagtcaccgg
      541 gcagggggcg ttaaaagccg ccacagagtt gcatgtgtac gctatcgggt cagagcgcga
      601 tcaggcggcg gttttagggg tacaaggctc gagcgtggtc acttcggtat atgggcagag
      661 tcagttgacg gttcaggatg tgatgcgaat gctccgtgag ggaaaactct ctaagacatg
      721 gcaaggctcg gtggggtata tcccgctgag ttcggcgtat ccgcctgagg ttagtttggg
      781 gttagacctg ttattggggg ctttaaagcg aggggaaatc caaacaaatg tcacaaaatc
      841 tcattaacat tgcgcagtct tgcacaaaga ttggagtaga tttatacttg ttaggtcgtg
      901 aggctgtacg aaaacagtct ttccaatcac tatcttctag aggagaagac ataaaatgaa
      961 gaagctttat tttgttatgg ctgctttgat catcgcttcg atggttttgg ctgcctgtgg
     1021 tactcctgct gccactgaag cccccgctgc tactgaagct gccgctaccg aagctgctgc
     1081 taccgaagcc cctgccgcta ccgaagctgc cgctgccacc atcaaagttg gtgaagtgac
     1141 cgacctcggc ggtattgatg acaagtcctt caatgccaat ggttgggctg gcgctcaaaa
     1201 agccgctgcc gaactcggtg tggaagccaa atatctcgaa tccacccagc aggctgatta
     1261 cgccaagaac attcagcagt tcgttgacga aggcaccaac ttgatcgtca ccgtcggctt
     1321 cttgctcggc gttgataccg ctaccgctgc caaggcgaac cctgatactc acttcgcgat
     1381 cgtagactat gcttaccctg actgcttcgg cacccaggtt gaaggcaagg actgcggttc
     1441 cgcgaccgaa ctccccaacg ttctcggttt gaccttccag atcgaccagg ctggcttcct
     1501 cgctggttac gctgctgccg cctccaccga gaccggtaaa gttgccacct tcggtggtat
     1561 caacattcct ccggttgctt ccttcatgat cggtttcgaa aagggtgttc tgtactacaa
     1621 cgaacagaac ggcaccgctg tggaagtcct cggctggaac actgaagcca acgatggttc
     1681 cttcaccggt aacttcgact ccctcgatga tggtcgctcc ttcgccgaat ccttcgttca
     1741 ggaaggcgct gacatcatca tgcctgtggc tggacctgtt ggtctcggct ctgctgctta
     1801 ctgccaggaa tccggcgcct gcaagatcat cggcgtggat gttgactgga ccgtttctgc
     1861 tcctgaatac aaggacatca tcctcaccag cgttctcaag aacatcaacg ttgctgtgta
     1921 cgatgccatt caggctgaag ttgacggcac ctttgctggt ggcgtgtatg ttggtaccgt
     1981 tgaaaacggc ggcgtcggta tctccgatgt tgctggtgct tctgacgacc tcaaggctca
     2041 acttgacgct gtcacccagg gcatcatcga cggctccatc gcaaccagat aatctggctc
     2101 tactgcacta caaaagccgg aggaggggtt tccctccccc ggctttttat ttttcttatt
     2161 gctcgtagat ctcaaaggtt ttttcaattt gtcatacatt ttccatgcac catcctttat
     2221 aattggggcg tgcaaaaaaa accttttgga tctatcaaag taaagatagt ggagggtatg
     2281 gcatgaccaa tgttttggaa ctgcgtggca ttaccaaacg tttccccggt gttcttgcaa
     2341 acgacaaaat cagtattact ctgcgtgagg gaaaaattct cgctttgtta ggtgagaatg
     2401 gtgcaggcaa aagtacattg atgaacatcc tttatgggtt gtacaaacct gatgaaggac
     2461 agatcattgt ccgcggtaaa gaggtggata ttcaaggacc caacgacgcc atcgcacaag
     2521 ggatcggcat ggtgcatcag cactttatgc tcgtccctgt gatgacggtc accgagaatg
     2581 tgatgctcgg tattgaaccc acaaagaacg gtatattttt agacaaagaa aaggtcgcga
     2641 accgcatccg tgaaatttcg gatgaatacg gtttggaagt tgacccgaat gcctatatca
     2701 aagatctgcc tgtgggcatt cagcagcgtg tggagatcat taaagttctc tatcgccatg
     2761 cagatattct catcctagac gagccgacag cggtactcac cccgcaagaa gtggaaggct
     2821 tgttcaagat catcgatacg ctgatcaaag cgggcaaatc cattattttc attacgcaca
     2881 aattgaaaga agtgttggca gttgcagatg atattaccgt tctgcgcctc ggaactgtgg
     2941 tcggatcgat tgacccgaaa gaagcgactt ctgagatttt ggcaaccatg atggtggggc
     3001 gtgatgtgac cttggtggtt caaaagaaat tatctgaacc cggcagagct gttttggatg
     3061 tcaaagactt gtatgtgcga gatgaacgcc aaaacatgac cgttcagggc gtatctttcg
     3121 aagttcgtaa aggtgaagtc cttggcgttg ctggtgttca agggaatggg caaacggaat
     3181 tggtctatgc cctgaccggg ttgttacctc tggaagcagg tacgatccat ttgttggatg
     3241 aagccattca tcacacgtct ccacgtcaca tccttgaaaa aggcgtggcg cacattccag
     3301 aagatcgcca gcgccatggt ttgatccttt ccttccccat ccacgacaat atgatgttat
     3361 gcacctacta caaggaccca tttgcaaagg gcatttcctt gcagcagaaa accatttttg
     3421 aaaacgcaga ccaattggta aagcaatatg atgtgcgcac cccgaacatc tatgtcaatg
     3481 cagggacgct ttccggtggt aatcagcaaa aagtgatcgt ggcgcgtgaa ttttcacgtc
     3541 ccattgaatt attgatcgcc tcacaaccga cccgtgggtt ggacgttggc tcgattgaat
     3601 acatccactc acagattatc aaaaagcgcg acgaaggttc tggcgttctg ctcgtttcat
     3661 ccgaattgga tgaaattctc gctctctcag accgtatcgc tgtgatgtac aaaggtcaga
     3721 tcatggcgat cgttgatgca agcaaagcga ccaaagaaca acttggtctg ctcatggcgg
     3781 gtgttcatcc ctccgaaccc tcgaacaaga attaggagtt gtatctgtga ctactacagc
     3841 tactcccgcc tcagatgctg ggacaaaaga a
//
LOCUS       DAOREF010000172         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14377_length_3859_cov_4.344900, whole genome shotgun sequence.
ACCESSION   DAOREF010000172 DAOREF010000000
VERSION     DAOREF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14377_length_3859_cov_4.344900"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKI33_04810"
     CDS             complement(<1..994)
                     /locus_tag="PKI33_04810"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNM36356.1"
                     /translation="MRKRVVVTGLGCVSPVGNNVKDTWEALLAGKSGAAPITAFDASA
                     HKTKFASEVKGFDGAALFGTRESRKMDRFTQFATAATLEALEHSGLKIDESNRDRVGI
                     LIGTGIGGIITTIEQYENLKERGPERVSPFLIPMMIADSAAGNLAIRIGARGPNMSLA
                     TACASGTNGVGEAAEMIRRGAADVMVAGASEAAIHSLTMAGMNVMTALSTRNDDPQRA
                     SRPFDKDRDGFLMGEGSAVLILESLDHALARGANILCEFTGYGTSDDAHHISAPAENG
                     AGAAISMSLALKDAGFGVADIQYINAHGTSTQLNDKSETAAIKTVFGEQAYKLAV"
     gene            complement(1146..2339)
                     /locus_tag="PKI33_04815"
     CDS             complement(1146..2339)
                     /locus_tag="PKI33_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM36357.1"
                     /translation="MKLTLLLDLDETLLNTNQDVFFPAYIQALSTNFASQLDPKLVMR
                     ALMTSIEKMNQNDDASRTLKDVFSDEFCPQVGMTRQELDAHIDVFYSQVFPTLRGITS
                     QIAETQPFIEWAMKQGYRLVVATDPLLPRPATRQRVQWAGLDPDQFELISTFEDFHFL
                     KNFSAYYAEVLGQLGWQEGPVLMVGNDAERDILSAKRLGLATFFVETESGSTSGPEAG
                     PRGTLSDLRRYLESTDLASLTPSFKTKEAIMAIQASVPAVLDTFFRGLSAETWKHKPA
                     SSEWTLTELVCHLRDTEREIHHMQLKLFNGQDEPFIPRPDTGIWANQRDYLHEDGPSA
                     LHEFTEARSMTLSMLQNTPDVEWDRKARHAIFGPTNFREVAGFIADHDRLHVQQALSL
                     LKKLQ"
     gene            complement(2370..2702)
                     /locus_tag="PKI33_04820"
     CDS             complement(2370..2702)
                     /locus_tag="PKI33_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36358.1"
                     /translation="MKTPAGHECPHFYGDYFRGRNVEECRLLKSQNQAWSPDLCKTCP
                     VPAIARANSCQFMKLNVVVARPLTAIFQRRVQVQAYCEKSNQNVSEPQVGCGECHALP
                     FKFEIKED"
     gene            complement(2708..3265)
                     /gene="efp"
                     /locus_tag="PKI33_04825"
     CDS             complement(2708..3265)
                     /gene="efp"
                     /locus_tag="PKI33_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNM36359.1"
                     /translation="MIDVNELRKGVTFELDGNLYKVLDYSHNKTGRGNATIRVKARNL
                     LTGANIERTFNSGQSVQDVSLDFANVSYLYSDGDLYYFMDKVTFEQPGIKAESLGESA
                     QYLKEGMEVKLTFYKGEAIDVEMPTSVDLKVVEAEMAIRGDTATGVTKKVTTETGLTV
                     QCPNFVNVGDTIRVDTRTGEYVTRV"
     gene            complement(3423..>3859)
                     /locus_tag="PKI33_04830"
     CDS             complement(3423..>3859)
                     /locus_tag="PKI33_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNM36360.1"
                     /translation="AWSSNGKQIAYIKEVNGATDLYVIDLETGTERQLTNDGKTTNAV
                     WSPEGDRIAYQSERSGNLDIYSYDFRDNKEYRVTDFDGKDSGPTWDCGGTNLAFTSVR
                     DGDLNIFQVFWQGGSAGNMTIDPASDKWSQWRPSNDISSVGY"
BASE COUNT         1009 a          945 c          880 g         1025 t
ORIGIN      
        1 atacggcaag tttataggct tgttcaccaa acacagtttt gatcgcagca gtttcacttt
       61 tgtcattcaa ctgcgtagat gtgccatggg cattgatata ttgaatatct gctacaccaa
      121 agccagcatc tttcaatgcc aaactcatgg aaatagcagc acctgcccca ttttctgctg
      181 gagcagaaat atgatgcgcg tcatctgaag taccgtagcc agtgaattca caaaggatat
      241 ttgccccacg agctaaggca tggtccaaag actctaagat caatactgct gagccttcac
      301 ccatcaagaa cccatctcgg tctttatcaa acggacggga agcacgctgt gggtcatcat
      361 tgcgagttga aagcgcagtc atcacattca tgcctgccat tgtcaacgaa tgaatggcag
      421 cttcagaagc gcccgctacc attacatctg cagcgccacg gcgaatcatt tctgcagctt
      481 cccccacacc attcgtgcct gaagcacaag cagttgccaa ggacatgttt ggtccacgtg
      541 cgccaatgcg aatggcaagg ttgcccgctg cgctatctgc aatcatcatt gggatcaaaa
      601 atgggcttac acgttctggt ccacgttctt tgagattctc gtattgctca atggtggtaa
      661 taatgccgcc aatgccggtg ccgattaaaa taccaactcg gtcgcggttc gattcgtcga
      721 tcttcaagcc tgaatgttca agtgcttcca acgttgccgc agtagcaaat tgggtgaagc
      781 ggtccatttt gcgagattca cgggtgccaa ataaggcagc tccgtcaaag ccttttactt
      841 ctgaggcaaa cttcgtctta tgagcgctgg cgtcaaatgc agtaataggg gctgcaccag
      901 acttgcccgc aagcaaagct tcccaagtat cttttacatt atttccaacc gggctaacac
      961 accccaatcc cgttacaaca actcgttttc tcatgtacca gtactcctta tcgttaattt
     1021 gcaaaaaata gaacaagcaa acttgtccta tttaacacaa gattcgggaa ttggacacgc
     1081 gggaatatgg cacctgggct taaaaaacca ggtcatcaga caatattagg aggcattccc
     1141 aatcgttatt gaagtttttt cagcaaagaa agagcttgtt gaacatgtaa tctatcgtga
     1201 tctgcaataa aaccagccac ttcacggaag ttggttggtc caaaaattgc atggcgcgct
     1261 ttgcgatccc actctacatc tggcgtattt tgtaacatac ttaatgtcat agaccgtgcc
     1321 tcagtaaatt catgcaatgc agaaggacca tcttcatgta aataatctcg ttggtttgcc
     1381 caaatccctg tatctgggcg tggaatgaac ggctcatctt gtccgttgaa aagcttcaat
     1441 tgcatgtgat gaatctcgcg ctctgtatca cgcaaatggc agaccaattc tgtcaacgtc
     1501 cattcgctgg aagcaggttt atgtttccat gtctccgcac ttaatccgcg gaagaaggtg
     1561 tctaacacgg caggtacaga tgcttgaata gccatgatag cttcttttgt tttaaaggat
     1621 ggagttaacg atgcgagatc ggtagactca aggtaacggc gtaggtcaga taatgtgcca
     1681 cgcggtcccg cctcaggtcc agacgttgag ccggattctg tttccacgaa aaatgtagcc
     1741 agcccaagcc tcttcgctga aagaatgtcc cgctctgcat cattccccac catgagaact
     1801 ggaccctctt gccagcccaa ttgcccgagc acctcagcgt agtaggcaga aaagtttttc
     1861 agaaagtgaa aatcttcaaa agtagaaatc aattcaaatt ggtcagggtc tagcccagcc
     1921 cattgcacac gttgacgagt cgctgggcgt ggcaataacg ggtcagtcgc cacaaccaaa
     1981 cgataccctt gcttcattgc ccattcaata aatggctgcg tctctgcaat ttgtgaagtg
     2041 ataccgcgca aagttgggaa cacttgacta taaaacacat caatgtgtgc atctagctct
     2101 tggcgcgtca tccccacttg cggacagaat tcatcactga acacatcttt caatgtacgg
     2161 gaagcatcgt cattttgatt catcttttcg atgctcgtca ttaacgcacg catgaccagt
     2221 tttggatcta actgcgatgc aaaattggtc gacaatgcct gaatgtacgc agggaaaaag
     2281 acatcctgat tggtgttcag cagggtttcg tcaaggtcta gcagtaatgt gagcttcatg
     2341 atgaaaaaga ctcctacgga gtctttgggt taatcttctt ttatttcgaa tttaaaaggc
     2401 agggcatggc attcgccaca ccccacctga ggttcagaaa cattctggtt tgatttttcg
     2461 caataggctt gcacttgcac tctacgctga aatatagctg ttagtggacg agccaccacc
     2521 acattcaatt tcatgaattg acatgaattc gcgcgtgcaa tagcgggcac agggcaagtc
     2581 ttacaaaggt ctggagacca ggcttggttt tgcgatttaa gcaagcgaca ctcttccacg
     2641 tttctgccgc ggaagtagtc gccataaaaa tgcgggcact cgtgcccagc aggtgttttc
     2701 attcctatta gacgcgggta acgtactcgc ccgtacgagt gtctacacga atggtgtcgc
     2761 caacgttgac gaagttcggg cattgcacag tgagcccggt ttcagtggtg accttcttgg
     2821 ttacgccggt ggcagtatcg ccgcggatcg ccatttcagc ttccacgact ttgaggtcca
     2881 cagaagtagg catttccaca tcaatagctt cacctttgta gaaggtcaac ttaacttcca
     2941 tgccttcttt gaggtactgg gcgctttcac caagtgactc ggctttaatg ccaggctgtt
     3001 cgaaagtgac cttatccata aaatagtaaa ggtcgccgtc actgtagagg taagaaacat
     3061 tagcaaaatc gaggcttaca tcctgcacgg attgacccga gttgaaggtg cgttcaatgt
     3121 tggcaccggt aaggagatta cgcgccttaa cacggatggt cgcattgccg cgcccggttt
     3181 tgttgtgtga atagtccaac accttataga ggttgccgtc caattcaaaa gtgacacctt
     3241 tgcgaagttc atttacatcg atcattcttg cattttcctt ggttatttgg tttttgtata
     3301 agcgggcgga ttataccaac gagtagcgaa tcatggctag gatgggatca ttaggaaaga
     3361 atgaggcaca aaaaagagcc tctcctcaaa aaacaggaga ggctctttgc gatcaaatct
     3421 tattagtagc ccacacttga gatgtcattg ctaggtcgcc actgtgacca cttatccgaa
     3481 gcggggtcga tggtcatgtt tccagcggaa ccaccctgcc agaagacctg gaagatgttc
     3541 aagtctccgt cacgaaccga tgtgaaggcg aggtttgtgc caccacagtc ccatgtcgga
     3601 cccgagtctt tgccatcaaa gtccgtcaca cgatactcct tgttatctct aaagtcatag
     3661 ctatagatat ccaagtttcc actgcgttca gattgatagg caatacggtc accttcagga
     3721 gaccaaacag cattggtcgt cttgccatcg ttagtgagtt gtctctctgt tccggtttcg
     3781 aggtcaatca catacaggtc tgttgcaccg ttgacttctt taatgtatgc aatctgcttt
     3841 ccgtttgaag accaagctg
//
LOCUS       DAOREF010000173         3852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14421_length_3852_cov_3.701080, whole genome shotgun sequence.
ACCESSION   DAOREF010000173 DAOREF010000000
VERSION     DAOREF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14421_length_3852_cov_3.701080"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKI33_04835"
     CDS             <1..220
                     /locus_tag="PKI33_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36361.1"
                     /translation="KGSGSSGKNAGGHYYLPLYMHEQIHTSVKVAKAAPAKKAAAKKT
                     VAKKVTTKKVAAKKTVKKAVKKTAKKKK"
     gene            274..666
                     /locus_tag="PKI33_04840"
     CDS             274..666
                     /locus_tag="PKI33_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36362.1"
                     /translation="MRVIKQSFQIIHYLIFMVGILFISACSNELNTPTPVATENVPTF
                     NVQIPDKALLKNYLVVNLETEPNTTCELTYIAPKGQKLHMIETADKNGICEWRWKLEE
                     SDGRGHGRLIFTINGVSETHFIDIRPGF"
     gene            737..1810
                     /locus_tag="PKI33_04845"
     CDS             737..1810
                     /locus_tag="PKI33_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36363.1"
                     /translation="MEQELKLRDYPIIGWLVGLGGLGWGGYTFFNTLPAFNLNAALVG
                     FGGLLFLVLSYSLVVTADKTTGLLTLDYRSIFHHSTKEIPLSQISNIRVDRSRTRSSK
                     GGSSTTYRVEAILSSGEVVPFRSYHTGNLFKHQKWANELRAFLNISEGFDETPQGFFK
                     AAQQAGATIAQRQQEALTGSNAAMRNTDGVNWQLQSFGMGATPGTRWYSPDFKTQGSF
                     LFLAQKIAGQASTGFLASLGKTLFKQSISLYGFDVSDTPNIEKADTYASLSAALDTHF
                     SAFTSNQTEAQQILNPWTQQPLADWGVKYPLQQFQSNARFSQLVVMFNPKGIYIATLG
                     TFPEDKLEEITRLGVELVKAHGS"
     gene            1916..2347
                     /locus_tag="PKI33_04850"
     CDS             1916..2347
                     /locus_tag="PKI33_04850"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNM36364.1"
                     /translation="MYTPKPLKWDDALSTGNQTLDEQHKYLIDTLNSLGDAIIEGHGI
                     DAIARILGRLRFYAGWHFGREEECFESYHCPAAEKNKKAHAVFIEKFDKYQDKFHESG
                     GSLDLALKVHEEISDWIVNHIMIVDGELYPCIHNHPKPLKK"
     gene            complement(2389..2661)
                     /locus_tag="PKI33_04855"
     CDS             complement(2389..2661)
                     /locus_tag="PKI33_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36365.1"
                     /translation="MQTDPASLDPEERLNKFYALLSAGFGVLSLCAGLIPMAGTALGI
                     AGIVFGLLGRRSENKRLAVVGIILSIIGVLTAIIYSILLYRAKYGA"
     gene            complement(2664..>3852)
                     /locus_tag="PKI33_04860"
     CDS             complement(2664..>3852)
                     /locus_tag="PKI33_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNM36366.1"
                     /translation="EQRTRYDLEMLKEVGYCSGIENYSRHLDGRPAGSHPWTMIDFLP
                     SDYLLILDESHMTVPQIRGMYNGDRARKETLVEYGFRLPSALDNRPLKFDEFEQVMGS
                     TIYTSATPADYEMKHAEQVVEQIIRPTGLIDPEVVVRPVKGQVDDLVGEIKERVERGE
                     RILVTTLTKRMAEDLTTYMKELGIKVNYLHSEVETLERVGILRDLRLGNFDVLVGINL
                     LREGLDLPEVSLVAILDADKEGFLRSDTALIQTIGRAARNVNGRVIMYADRMTDSMKR
                     AIDETNRRRAKQVKYNEEKGIVPFSITKAIHDLTEEMSAKAVGEMKGEYKVKNKNEMP
                     RNELRQVMHEMEKQMKDAAKNLEFEKAAALRDELYELKSLLAEDESLKPWERIKLLSG
                     EEE"
BASE COUNT         1112 a          994 c          789 g          957 t
ORIGIN      
        1 gaaaggctct ggctcatctg gtaagaatgc aggcggtcac tactatctgc cgctctacat
       61 gcatgagcag attcacactt cagtaaaagt tgcgaaagcg gctcccgcaa agaaggcagc
      121 agcgaagaaa actgtagcaa agaaagtaac tacgaaaaaa gtagctgcaa agaaaacggt
      181 caagaaagcc gtgaagaaga cagctaagaa aaagaagtaa tctgaaatta gcctctcgag
      241 tacgctcggg aggctttttg atagaattct cctatgagag tcataaaaca aagttttcaa
      301 atcattcact atcttatttt tatggttggt atattgttca tctccgcttg tagcaatgaa
      361 cttaacacac cgactcctgt agccacagag aatgtgccga cctttaacgt ccaaatccca
      421 gataaagctc tcttaaaaaa ttatctcgtt gtcaatttgg aaacagagcc aaacacaacc
      481 tgtgagttga cctacattgc ccccaaagga caaaaacttc acatgattga aaccgctgat
      541 aaaaacggaa tctgtgaatg gcgctggaag cttgaagaaa gcgatggcag agggcatgga
      601 cgtctgattt tcaccatcaa cggcgtcagc gaaacccact ttatcgacat ccgcccgggg
      661 ttttagatag gagctttgtc tggtttgtat ccattgaaaa tttcttataa ttaaataaaa
      721 acagaggagc aacataatgg aacaggaact caagcttaga gattatccga tcattggttg
      781 gctggtaggg cttggcggac tcggttgggg tggctatacc tttttcaata cattacctgc
      841 tttcaacctg aatgccgccc tagtggggtt tggagggcta cttttcctag tcttaagtta
      901 ctcactcgtt gtcactgccg ataagaccac cggcttactc actctcgact atcgctctat
      961 ttttcatcat tccactaaag aaattccgct cagtcagatt agtaatatca gagtagaccg
     1021 ttctcgcaca cgcagcagca aaggcggatc aagcacaacc tatcgtgttg aagcgattct
     1081 atcaagtggt gaagtcgttc cattccgttc gtatcacacc ggaaatttat tcaagcatca
     1141 aaaatgggca aatgaattgc gggcatttct caatatttcc gaagggttcg acgaaactcc
     1201 gcaaggcttt ttcaaggcag cacaacaagc tggagccacg atcgcacagc gtcaacaaga
     1261 ggcgctcaca ggctccaatg ccgccatgcg caacacagac ggtgtcaact ggcagttaca
     1321 gtcctttggt atgggcgcaa cgcccggcac acgttggtac tcgcctgatt tcaaaactca
     1381 aggtagtttt ctctttttag cacaaaaaat tgccgggcag gcaagtacgg gcttccttgc
     1441 ctctctcgga aaaacccttt tcaaacagtc catttcgctt tatggattcg acgtctcaga
     1501 cacaccaaac atcgaaaaag cggataccta cgcctctctc tccgccgcgc tggatactca
     1561 cttctcagcc tttaccagca accaaactga agcgcaacaa attctcaacc cgtggaccca
     1621 gcaaccgttg gcagactggg gcgtgaaata tccgttacag caattccaat ccaacgcaag
     1681 attcagtcaa ctcgtagtaa tgttcaaccc caaaggcatc tacatcgcta cactaggaac
     1741 cttcccagaa gacaagcttg aagaaattac ccgtcttggc gtggagttgg tcaaagcaca
     1801 tgggagttaa aaataggaca atcgtcctcc gaatttcatt acaatgatac gaagtctcca
     1861 gagagacccc ggaatataat ttgaatctaa atcagttgtc tttctggaga aaaaaatgta
     1921 cacacccaaa cccctaaaat gggatgatgc actcagtact ggcaaccaaa ccctcgacga
     1981 gcaacacaag tacctgatcg ataccctaaa tagccttggc gatgcaatta ttgaggggca
     2041 cggaattgac gccatcgcca gaattctagg caggttgcgc ttctatgctg gctggcattt
     2101 tgggcgcgaa gaagaatgct ttgaaagcta tcactgcccg gcagccgaaa agaacaaaaa
     2161 agcacatgcc gtgtttatcg aaaaattcga taaatatcaa gacaaatttc acgaaagcgg
     2221 tggctcgctt gatttagcgc taaaagtcca tgaagaaatt tcagactgga tcgtaaatca
     2281 catcatgatc gtggacggcg agttatatcc ttgcattcat aatcacccca agccgctcaa
     2341 aaaataatat aaaagagccg cagaattctg cggctctttt cgttgtaatc aagcaccata
     2401 ttttgctcga tacaaaagaa ttgaataaat gatggcagtc agcaccccaa taattgataa
     2461 gataattcca accactgcca accgtttatt ttcagaccgc ctgcctaata aaccaaagac
     2521 aataccggca attcccaacg ccgtccctgc catgggaatt aatccggcac ataaactcaa
     2581 taccccaaaa cctgccgaaa gcagggcata gaacttattg aggcgctctt ccgggtcaag
     2641 agaagcaggg tctgtttgca tggctattct tcttcaccag atagtaattt gatacgttcc
     2701 caaggcttca ggctttcatc ctctgccaat aagcttttca actcatacag ctcatcgcgc
     2761 agagccgccg ccttctcaaa ctcaagattt ttcgcagcat ctttcatctg cttttccatc
     2821 tcatgcatta cctggcgcaa ctcgttgcgc ggcatttcat tcttattctt aaccttatac
     2881 tcgcccttca tttcgcccac cgccttggca gacatttcct cggtcagatc atgaattgcc
     2941 ttggtaatac taaacggcac aatacccttt tcttcgttat atttcacctg cttggcacgg
     3001 cgacgattgg tttcgtcaat ggcgcgtttc atcgaatcgg tcattcggtc agcatacatg
     3061 atgacacgtc cattcacatt acgcgccgca cgcccaatcg tttggatcaa tgcagtatca
     3121 gagcgcaaga aaccttcttt gtccgcatcg agaattgcca ccagcgacac ttcgggcaag
     3181 tccaaccctt cgcgcaaaag gttgataccc accaacacat caaaattacc aaggcgtaaa
     3241 tctcgcaaga tccctacgcg ctccagcgtt tccacttcag agtgcaaata attaaccttg
     3301 atgcccaact ctttcatgta agtagttagg tcttctgcca tgcgtttggt aagggtagtg
     3361 acgaggatac gctcgcctct ttctacccgc tccttgatct ctccgaccaa gtcatctacc
     3421 tgccccttga ccgggcgcac cacgacttca gggtctatca accccgttgg gcggataatc
     3481 tgctccacaa cctgctcagc atgcttcatt tcataatcag ctggcgtcgc agaggtgtaa
     3541 attgtggagc ccatcacctg ctcaaattca tcgaacttga gcggacgatt atccaatgca
     3601 gatggtaaac ggaaaccata ttccaccaat gtctctttac gagcgcgatc gccgttatac
     3661 atgccacgta tttgcggcac agtcatatgg ctctcatcta ggatcaacaa ataatcgctg
     3721 ggcaaaaagt caatcatcgt ccatggatgt gaaccggcag ggcgtccatc caaatggcga
     3781 gaataattct caatccctga acaatagcca acttccttaa gcatctccaa gtcatatcgg
     3841 gtacgttgct ca
//
LOCUS       DAOREF010000174         3845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14466_length_3845_cov_4.576253, whole genome shotgun sequence.
ACCESSION   DAOREF010000174 DAOREF010000000
VERSION     DAOREF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14466_length_3845_cov_4.576253"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..604
                     /locus_tag="PKI33_04865"
     CDS             116..604
                     /locus_tag="PKI33_04865"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HNM36367.1"
                     /translation="MILDLPLILETRRNHALEHATIHLLSHKHPGKRMAGHSNPTGFF
                     LLGDLTTQQIWESATEAHTRLNSGESGLAVHPGCGTNMATTALLAGTFAWFPLRGTKS
                     TLWRLMLVPFALLFALAGYQLSKPLGPWLQKYVTTEANLGSLQIIDIVPVRKGVHRVI
                     TK"
     gene            666..1637
                     /gene="holA"
                     /locus_tag="PKI33_04870"
     CDS             666..1637
                     /gene="holA"
                     /locus_tag="PKI33_04870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNM36368.1"
                     /translation="MPTIFLLYGNDEFAITRKLKDFESDFTDPTTADMNTTRLEARSM
                     TENDLNNAVNAMPFLAPKRLVFIAYPSGKFTKPDARKKFQEFLEKAPDTSKVVLYDVV
                     EKPKDAEKHWLVKWAEKNKPQVRSQAFFLPQQRDMPGWIINEAKNQGGQIEPAAAARL
                     AEMSGVDTRQAGMEISKLLAYVNWERPVRGSDVEAVCIVTSQQSVFDFVDALSQGNAK
                     TAQRLLHRLLENEDPFSLWGMVVRQFRLLLQAREILDARGNKDDVARALAIHPYVAEK
                     TTGQAGRFSMEALEGIYHRLLRIDEQVKTSQVTLDLALDTLVVELAR"
     gene            1680..2294
                     /locus_tag="PKI33_04875"
     CDS             1680..2294
                     /locus_tag="PKI33_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNM36369.1"
                     /translation="MRRTKEEAAVTRATLLKAALTLFSTKGYAATSIDDITQAAKLTR
                     GALYHHFTNKADLYNTLIEDVSARGADIVQGAVAEGGTFLDILKRVFVRQLAYIEESK
                     EARAVMELALVKTGAHPELQSGRDRQIESGKLMVASLAQTMQIGIASGELREDIPPME
                     MARSFIAFQNGVIQLWLASPKSFSLKGRAAVFADVLIQGLKKIA"
     gene            2350..3177
                     /locus_tag="PKI33_04880"
     CDS             2350..3177
                     /locus_tag="PKI33_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNM36370.1"
                     /translation="MYKSISEWMRTNPIPAFFLLTFVIAWMIWLPIGYLAPKYFLLAI
                     IPGAWAPTIVSVLLTWLTDGKSGVRQFLSRVIRWRVSFQWYLVVLFGIAVIAFAAQGL
                     NIWLFDGEPVKLNLPAGIPENGLLIALPIIFFVNIFFGGPLAEDIGWRGYALPKLREK
                     MNAFTASLLIGIVWVLWHLPFFWIPEGKIAVGGIPLLWFALLTTAWSVLFAWVYVNTE
                     SILMPVLFHAAVNTTLGTLGILGQANGSLTPVILNTVLTWLLVGVIVFFTGRDLKKK"
     gene            complement(3221..3535)
                     /locus_tag="PKI33_04885"
     CDS             complement(3221..3535)
                     /locus_tag="PKI33_04885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNM36371.1"
                     /translation="MYIFQVHHYIKPEGIDSYKAVTLENVQKTRLEPGVLRFEFFQDA
                     VNPCHFSLFEVYADMAARDAHLQTEHFLKWKDVYLATQERKGDGHEFISVYPEEGEWK
                     KD"
     gene            complement(3609..>3845)
                     /locus_tag="PKI33_04890"
     CDS             complement(3609..>3845)
                     /locus_tag="PKI33_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36372.1"
                     /translation="GMDGETAGYAVVLGGSVIMMVLTILLTLSGIVTGALALRNKDSK
                     RGLAIAGLVLNFLCFAPYCLLLALMAFSAIPTGN"
BASE COUNT          971 a          925 c          957 g          992 t
ORIGIN      
        1 gatttacgac tcaccgatta tgcaggtcta acatataata cccacggttg aaagttgtgc
       61 tttctgtcct ctaaaaaagc gcaactttca accgagtgcg gtatataatc aacttatgat
      121 cctcgacctt cccctcattc tcgaaacgcg gcgcaatcat gcacttgagc atgccaccat
      181 tcatttgctt tctcacaagc atcccggtaa acgcatggcg ggacattcca accccaccgg
      241 attttttttg cttggcgatc tgaccacaca acagatctgg gaatctgcaa cggaagctca
      301 cacgcgactc aactctggtg aaagtggact cgctgtccac ccgggttgtg gaaccaatat
      361 ggcaaccacc gcgcttcttg ccgggacctt tgcctggttc ccgcttcgag ggaccaagtc
      421 tacgctgtgg cgtctcatgc tcgttccgtt cgcattgctc tttgcgttgg caggctatca
      481 actcagcaaa ccgctcggac cgtggctgca gaaatacgtc accaccgaag cgaatctcgg
      541 ctccctgcag atcatagaca ttgtgcctgt ccgcaagggc gtgcatcgag tgattacgaa
      601 ataaaaacgt aggggcggcg tcctgccgcc cttgggcgag gagacctcgc ccctacgaaa
      661 aaattatgcc caccatcttc ctcctctacg gcaacgacga atttgccatc acgcgcaagc
      721 tcaaagactt cgagtcagac ttcactgatc ccaccacagc ggacatgaac acgactcgtc
      781 tagaagcgcg ttccatgaca gagaacgacc tcaacaatgc tgtcaatgcc atgccgttcc
      841 ttgcgccaaa gcgtttggtg ttcatagcgt atccttcggg taagtttaca aaacccgatg
      901 cccgcaagaa atttcaggag tttttagaaa aagcccctga tacttccaaa gttgttttgt
      961 atgatgtggt ggaaaaaccc aaggatgctg aaaaacattg gctggtgaaa tgggctgaga
     1021 agaacaagcc gcaagtcagg tctcaagctt ttttcctgcc gcaacaacga gacatgcccg
     1081 gttggatcat caacgaagcg aagaatcagg gtgggcagat tgagccagcc gcggcggcgc
     1141 gactcgctga aatgtccggc gtggataccc gtcaggcggg catggaaatc tcaaagctgc
     1201 tggcatatgt caactgggag cgacccgttc gcgggtccga tgtggaggcg gtttgtatcg
     1261 tcacatccca acaaagcgtg tttgactttg tcgatgcgtt atcccaaggc aatgccaaga
     1321 cggcgcaaag gttactgcat cgtttgttag agaatgaaga cccattctcg ttgtggggga
     1381 tggtggtacg tcaatttcgg ttgctgcttc aagcacgcga aattttagat gcgcgcggta
     1441 ataaagatga tgtggcgcgc gcgctggcga tccacccata tgttgccgaa aagaccaccg
     1501 ggcaggcagg gcgtttttcg atggaagcct tggagggaat ttaccatcgt ctgctgcgga
     1561 tcgatgagca ggtaaaaaca agccaggtca cattagacct ggctctcgat accctggtcg
     1621 ttgaactggc acgataaatc tctcttgaca tacaattaca taccgattag tatgtataca
     1681 tgagacgtac aaaagaagag gcggcagtca cccgcgccac ccttttgaaa gctgccctaa
     1741 ccctttttag caccaagggc tatgcggcga cgtcaattga tgacatcacc caagctgcaa
     1801 agttgactcg tggtgcgttg tatcatcact tcaccaataa agccgacctt tacaacacgt
     1861 tgatcgaaga tgtctctgcc cgtggagcag acattgtgca aggtgctgtg gcggaaggtg
     1921 gaacgttcct tgatatcttg aagcgggtgt tcgttcgcca gcttgcttat attgaagaga
     1981 gcaaagaagc gcgcgctgtg atggaactgg cattggtcaa aacgggggcg catcccgaac
     2041 tgcaatcggg gcgagatcgg caaattgaat caggcaagtt gatggttgcg agcctggctc
     2101 aaaccatgca gatcggcatt gccagtggtg agttacgcga agatattcct cccatggaaa
     2161 tggctcgttc ttttattgca tttcagaatg gtgttattca gttatggctt gcttcgccga
     2221 aatctttctc gttgaagggt agggcggcgg tctttgccga tgtgttgatc cagggactta
     2281 aaaagatcgc ctaatttttt tattcaattt acatactaaa tagtatgtaa aagtaaaaag
     2341 gagaatacca tgtacaaatc aatttccgaa tggatgagga cgaacccaat ccctgccttc
     2401 tttttgttga cctttgttat cgcatggatg atctggctcc ccattggcta tctcgcgccg
     2461 aaatatttct tgctggcgat catccctggt gcatgggcgc cgacgatcgt atctgtattg
     2521 ttgacatggc tcacagatgg aaagagtggc gtccgtcaat tcttgagcag ggtcatccgc
     2581 tggcgcgtta gttttcaatg gtatctggta gttctttttg gcattgcagt catcgccttt
     2641 gctgcacaag gactgaacat ctggctattc gatggcgaac cggttaagtt aaatcttcct
     2701 gcaggtatcc ccgagaatgg attgttgatt gccctgccca tcattttctt tgtgaatatt
     2761 tttttcggcg gaccattggc agaagatatc ggctggcgtg gctatgcttt gccaaaactg
     2821 cgcgagaaaa tgaatgcctt cacagcaagc ctattgatcg gcattgtgtg ggtgctctgg
     2881 catttgcctt tcttttggat cccggaagga aagattgctg tgggcgggat tccgcttcta
     2941 tggttcgcgc ttctgacgac ggcgtggtct gttctgtttg cctgggttta tgtcaacaca
     3001 gaaagcattc ttatgccggt cttgttccat gctgccgtca atacaacgct tggaacattg
     3061 ggcatattgg gtcaagctaa tggaagtctg actcctgtca tcttgaacac tgtattgaca
     3121 tggctgttag taggtgtgat cgtattcttt accggcagag atttgaaaaa gaaataaatg
     3181 aagtaaaaaa agccagcttt ctaaaaagct ggctcaagtg ttaatctttt ttccattccc
     3241 cttcttctgg atagaccgaa ataaattcat gcccatcgcc tttgcgctcc tgcgtagcaa
     3301 ggtagacatc tttccatttc aggaagtgct cagtttgcag gtgtgcatct cgcgcagcca
     3361 tatccgcata gacttcaaag agactaaagt gacaggggtt gacggcatct tggaaaaatt
     3421 caaaacgtaa cacaccgggc tccaggcgag tcttttgcac attttccagc gtgacagctt
     3481 tgtaggagtc aattccttca ggtttaatgt aatgatgaac ttgaaatata tacatcgcag
     3541 cctccgattg ttgtttctac tttaatagaa acgaatagag gcaaactatg caatatcatt
     3601 gcaagaggtt aatttcctgt agggatggcg ctaaatgcca tgagtgccaa taaaagacaa
     3661 tagggagcaa agcatagaaa attaagcacc aacccggcaa tggcgagacc acgtttggag
     3721 tctttgttcc tcaaggcaag ggcgccagta acgattccag aaagggttag caagatggta
     3781 agaaccatca tgattacact gccacccaaa acaacggcat acccggcagt ttcgccatcc
     3841 atgcc
//
LOCUS       DAOREF010000175         3823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14595_length_3823_cov_4.829883, whole genome shotgun sequence.
ACCESSION   DAOREF010000175 DAOREF010000000
VERSION     DAOREF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14595_length_3823_cov_4.829883"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKI33_04895"
     CDS             complement(<1..648)
                     /locus_tag="PKI33_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNM36373.1"
                     /translation="MSKPKTLPYLEALFAVIVWGASFIATKIAVSEISPIAVVWVRFA
                     IGIPILFGAVLMRKQFVFPKGGEWLYFALLGFLGIAFHQWLQSNGLKTAQATTTAWIV
                     ATSPAFIAVLGWLVIKERLTALQSFGIALSMVGVLVVVSKGDLRSILGGRFGSVGDFL
                     ILISAVNWAVFSILSRRGLKQHPSTMLTLWVMTIGWLITTAAFVAGRNYAEIPAIT"
     gene            720..1550
                     /locus_tag="PKI33_04900"
     CDS             720..1550
                     /locus_tag="PKI33_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNM36374.1"
                     /translation="MMFYNARWYDPVLGRFAQADTIFPPGVQGLDRYAYVGNNPVRYS
                     DPSRHTSCEGDNWDDGPQCMSTNEFKDNSAKYNEVKKIQAAFKNIRIENILDWNLTDL
                     QEIYKALWMINKNGFNNHTDAFIKAFGDVIFTPVASGSLGSVGGNDVVANADYSNGLI
                     KVTSGASVDTIIHEMAHIFDGNLRRINPRVPFHSQDFVDAFNNSGSCTVAPCSGSGYS
                     PSGSTSVYGISDSDEDFADSFMFVITGQTQYIDSNRIQIINALLQSYTEINHILSPGR
                     "
     gene            1751..2203
                     /locus_tag="PKI33_04905"
     CDS             1751..2203
                     /locus_tag="PKI33_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36375.1"
                     /translation="MPNVEITNNGEYGTSWIFKDKGAVQVGSGDIQMNQDGIVKFLSL
                     GNATEYELTLIQVIEKYAEPSLVSPYYCREGLCDAIIIYPELGMALRIFAEDTGEGDR
                     HSIYVASDTVVKYVVFFSTNSKDKNLGGFVDNGLFEDFVSWKGYGVYP"
     gene            2288..3187
                     /locus_tag="PKI33_04910"
     CDS             2288..3187
                     /locus_tag="PKI33_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNM36376.1"
                     /translation="MTKYQYTGQYSHAADFGLMFYNARWYDPYLNHFTQPDSIVPDPY
                     NSQDYDRYAYARNNPLRYTDPSGHFTEDAVLEYLMTEFIEYGKNATLMAKKELAKWKS
                     DKQWWNMISMAKAGDVLFGRGNLNGGMDFAFTFTFNGSGNDSLTGITNEYGNGRSLGD
                     IKNGHTPLSGEMEMNWDWIGFIQLSEEEPGIYVRDGYSMTSERSEVWQRVVIGGTTDV
                     LITYGVAKLPIPPWAIAGLGGGMTYSDAAGFGPGTLMLDLADMQEFDAQVRISNEKGQ
                     GIYLNIQDHPFDGYMIEHAWTIH"
     gene            3216..3533
                     /locus_tag="PKI33_04915"
     CDS             3216..3533
                     /locus_tag="PKI33_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36377.1"
                     /translation="MNRKNRYGISFLISMLLPTTIVAVLSSLIGNDLTWLLPISIIIS
                     ILLGTEIEAEYDNKDSLRKLVFQTWQDKVSFMLALSVFLFILTLPLKEKINSPALLGL
                     FFV"
     gene            complement(3624..>3823)
                     /locus_tag="PKI33_04920"
     CDS             complement(3624..>3823)
                     /locus_tag="PKI33_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36378.1"
                     /translation="DYFQAMQRVEQRLEIVPSKQEENIEDVKVQMVQDSAKLLLWVER
                     LELPELCYEERLEIAGAPKGL"
BASE COUNT         1104 a          755 c          916 g         1048 t
ORIGIN      
        1 ggtgatggcg gggatctcgg cgtagttgcg cccggccacg aaggcggccg tggtgatgag
       61 ccagccgatg gtcatgaccc acagggtgag catggtggag ggatgctgct tgagtccgcg
      121 ccgtgagagg atggagaaga ccgcccagtt gaccgcgctg atgaggatga gaaaatcgcc
      181 cacgctgccg aacctgccgc ccaggatgga acgcaggtcg cctttgctga ccacgaccag
      241 caccccgacc atggagagcg cgatgccgaa ggattgcagg gcggtgagcc tttccttgat
      301 gaccagccag cccagcacgg cgatgaaggc gggcgaagtg gcgacgatcc atgcggtggt
      361 ggtggcctgc gcggtcttga gtccgttcga ttgcagccac tggtgaaagg caatgcccaa
      421 aaagccgagc agcgcaaagt acagccattc gccgcctttg gggaagacga actgtttacg
      481 catgagcacc gcgccgaaga ggatcgggat gccgatggca aaacgcaccc acaccacggc
      541 gatgggggag atctctgaga cggcgatctt ggtggcgatg aacgatgcgc cccagacgat
      601 cacggcgaac agggcttcga ggtacggaag ggttttgggt ttggacatgg tggttcctgt
      661 gcaagaagat ggggaattat aatgcgggga ggtgatattc ccacgccgcc gattttgggt
      721 tgatgttcta caatgcccgc tggtatgacc ctgtgttggg acgctttgcc caggcggaca
      781 cgattttccc gccgggtgtg caggggttgg acagatatgc gtatgttgga aataatccgg
      841 ttcgatattc agacccaagt aggcatactt cttgcgaagg agataattgg gatgatggtc
      901 cgcagtgtat gagcacgaat gaattcaagg ataattcagc gaaatataat gaagttaaga
      961 agattcaagc agcattcaaa aatattagga ttgaaaatat cctagattgg aacttaacag
     1021 acctccaaga aatatataag gcgctttgga tgataaacaa aaatgggttt aataaccata
     1081 cagacgcatt tatcaaagca tttggagatg taatatttac acctgttgca agcgggagtc
     1141 taggttcggt cggtggaaat gatgtcgtcg ccaacgcaga ctattcaaat ggattaatca
     1201 aagtcacatc aggagcatca gttgacacta tcatccatga aatggcacat attttcgatg
     1261 ggaatttaag aagaattaac ccaagagttc catttcattc acaagatttc gtggatgcat
     1321 tcaataattc aggttcatgt actgttgctc cttgttctgg aagtggatat agtcctagtg
     1381 gatcaacatc agtatacgga atatctgatt cagatgaaga ctttgctgac agtttcatgt
     1441 ttgtgataac tggacaaacg caatacatcg acagtaatag aattcagata ataaatgctt
     1501 tgcttcaaag ttacacagaa attaatcaca tattatcgcc aggtagataa ttatgaaaaa
     1561 aaacatattt gttttgattg ccgttttcct gcttttagtg atgtcttctt gcgctgcatc
     1621 atctatgtca aattctagat aaacatggtg gtattcttgg atagtacaac ctgcatgttc
     1681 gcctccatgt tgggagaaca ttattcctgg agaaactact atagaggaag ccataggagt
     1741 tttatccaaa ttgcccaatg ttgaaattac taataatggt gaatatggaa catcttggat
     1801 atttaaagat aaaggtgccg tacaagttgg cagtggcgat atacaaatga atcaagatgg
     1861 tattgtcaaa tttctctcat taggcaatgc tacggaatat gaattaacat tgattcaggt
     1921 aattgaaaaa tatgctgagc cttcacttgt ttcaccatat tattgcagag aaggattgtg
     1981 cgacgcgatt atcatatatc ctgaacttgg aatggcgttg cgaattttcg ccgaggatac
     2041 aggagaaggg gatcgacaca gtatttacgt tgcatcagat acagtagtaa agtatgttgt
     2101 tttcttctcg acaaacagca aggataaaaa tttaggaggc tttgttgata atggtttatt
     2161 tgaagatttt gtttcttgga aaggttatgg tgtctatcca taattaacca ttggtctcgt
     2221 tctcaacgca accttgagcg cgcccacaat ctccaatcta tcctcgtgac aaagttccgg
     2281 cagcgagttg accaagtacc agtacaccgg acaatactcc cacgccgccg attttgggtt
     2341 gatgttttat aatgcgcgct ggtatgaccc ttatcttaac cactttaccc agcctgacag
     2401 cattgtacca gacccgtaca attcacaaga ctacgaccgc tacgcttatg cgcggaataa
     2461 tcccttgcga tacaccgatc ccagcgggca ttttactgag gatgcagtcc ttgagtactt
     2521 aatgactgaa tttattgaat acggtaaaaa tgcaactttg atggctaaaa aagaacttgc
     2581 taaatggaaa agtgacaaac aatggtggaa catgataagt atggctaaag caggagacgt
     2641 gttatttgga agaggaaatt tgaatggggg tatggatttt gcatttacat ttacttttaa
     2701 tggatcggga aacgactcct tgacaggaat aactaatgaa tatggaaatg gcagaagtct
     2761 gggcgatata aaaaacggac ataccccatt gtcaggggaa atggagatga attgggattg
     2821 gataggcttt atacaattaa gtgaagaaga acccggtatt tatgtgcgtg atggatactc
     2881 aatgacttca gagcggtcag aggtttggca aagggttgtt attggcggaa ccacggatgt
     2941 acttattaca tatggtgtcg ctaaactccc aatacctcct tgggcaatag caggcctggg
     3001 aggtggcatg acatattcag atgctgctgg ctttggacct ggaacattga tgctggattt
     3061 agcagatatg caggaatttg atgcccaagt caggatatca aatgaaaaag gacaaggtat
     3121 ctatttaaac atccaagacc atccttttga tggatatatg attgaacatg catggacaat
     3181 acattaatct catcttcctg ttatggagag tcaatatgaa tcggaaaaat cgatatggga
     3241 taagcttctt aatcagcatg ttgcttccaa caacaattgt tgcagtctta tcaagcttaa
     3301 taggtaatga tttaacttgg ttgttgccta tttctataat aataagtatc ttattaggca
     3361 ctgaaattga agcagagtat gacaataaag attcgttgag gaaacttgtt tttcaaacat
     3421 ggcaagataa agtttctttt atgttagctc tctcagtttt tttgttcata ttgacattac
     3481 cactaaaaga aaaaataaat tcacccgctt tgctaggttt gtttttcgta tagggctttt
     3541 ttatctgcct cttaatcttt gcaatccttt caacttctaa aacaaaaaaa gaaattagga
     3601 gattcttact ccgttcataa tcttcaaagc cctttgggcg cccccgcaat ctccaacctt
     3661 tcctcgtaac aaagctccgg cagctccagg cgttccaccc aaagcagaag tttggcgctg
     3721 tcctgtacca tttgcacttt aacatcttcg atattttctt cctgcttgga tggcacaatc
     3781 tccaatctct gctccaccct ttgcatggct tggaaataat cat
//
LOCUS       DAOREF010000176         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14600_length_3822_cov_4.056278, whole genome shotgun sequence.
ACCESSION   DAOREF010000176 DAOREF010000000
VERSION     DAOREF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14600_length_3822_cov_4.056278"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKI33_04925"
     CDS             <1..365
                     /locus_tag="PKI33_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36379.1"
                     /translation="AGRMREHDLAVAILQDIPAFRTTYPNKVFDYMAAGRASIIVIDG
                     ITRDLIEASCGGVYVNPADDAMLAQKILELSHNPAQVKEMGANAREYLVKNLDRRDKL
                     DETLALLNSLTTKETKGH"
     gene            413..1276
                     /locus_tag="PKI33_04930"
     CDS             413..1276
                     /locus_tag="PKI33_04930"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNM36380.1"
                     /translation="MKVLVTGATGFTGSRLVPLLLKSGFEVRVFARPTSDRSPLSALT
                     VEWATGDFANPETFTAALRGADALVNIASLGFGHAESILKSMKEAGVKRGLFISTTAI
                     FTQLNAVSKSIRLAAEEAIQTSGLDYTILRPTMIYGSKRDRNMWRLIRLLRITPIMPI
                     FGDGESLQQPIFVDDVAQAVLLALKNDMTIGKSYNIAGKAPLSYNQVIDTVASALGKR
                     VWKLHMPYMTIVRALQFTERMKLRLPIKAEQVLRLNENKAFSYEEAQKDFDFNSRSFE
                     EGIRTEIQANK"
     gene            1550..>3822
                     /locus_tag="PKI33_04935"
     CDS             1550..>3822
                     /locus_tag="PKI33_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM36381.1"
                     /translation="MPKNKTKMDIQAELEAAQKRVAELEEAFGSDRVEQRFASVFRAS
                     PSQMALTDQTTGRYIEVNEAFLNTLGFSREEVLGKTATELNLFVDPQQRQDLLLRMRD
                     QGFLRNEHVLVRAKTGEIRHGIFSAEFINTTEQKLLLTVMNDVTEKLQAEQRWQFALD
                     GADEGVWDWDPQTNKVFYSKRWKSMLGYEDGEIGNTLEEWSSRVHRDDLPRVMQEVTR
                     HLSGETPIYKSEHRILRKDGTYLWVLDSGKVMEWSEDKKPLRVIGTHKDISERKKAEE
                     SLRENEERFSRMFHANPAVQLIVKFDDGIVLDVNESFCLQTGFDRDELIGRSMRDLNL
                     WQDPFKQKRIMDELRSKGGVHDVELEFWTNTGDLRTLLISFDLVEIRGVLCNLATGVD
                     ITSRKQAERDLRFREKQYHSLVETLDVSICRWLPDTTLTYANEKYRKIFNITGNAEGK
                     KWIDLLPEAARASTAIFYSEVTKHPRTVMYDHPVTMNDGKIHHYHWVDTPILGNDGEV
                     LEFQSIGIDITDRKLSENALVASEKRLKSLVDSQSHYVIRVNMEGRYSYWNAQFEKEF
                     GWLYESEGIQNAFVLKAVCSYHHERVTNIVNQCIAEPGKVFTVEIDKPARNGSIRTTL
                     WEFICLTDELDQPYEIQCMGVEITGRKQAEDDLRKNEQMLRLFIEHSPAAIAMFDLEM
                     RYIIASKRYLIDYRLGDVDLVGRSHYEIFPEMTEEHRHIHQRCLMGEIDKKDEDLLVR
                     NDGTSDWVRYELRPWHQS"
BASE COUNT         1081 a          807 c          928 g         1006 t
ORIGIN      
        1 gcgctggccg aatgcgtgag catgatctgg ccgtcgccat cctgcaggac atacctgcct
       61 tccgcaccac ttaccccaac aaagttttcg actacatggc agccggtcgc gctagcatca
      121 tcgtcatcga cggcatcacc cgcgacctta tcgaagcctc gtgtggcggc gtctatgtca
      181 accctgccga tgacgccatg ctcgcgcaaa aaattctcga actctcacac aaccctgctc
      241 aggtcaaaga gatgggcgca aacgcccgcg aatacctcgt caaaaattta gaccgccgcg
      301 ataagttgga tgaaacactc gccctgttaa actctctcac cacaaaggag accaagggtc
      361 actaaggttt ttcctttgtg tcactttgtg acccttcgtg gttaaatctt ttatgaaagt
      421 tcttgttaca ggtgcaactg gatttaccgg ctcacgcctt gtccctctcc tcctcaaaag
      481 cggattcgaa gtccgcgtct ttgctcgccc cacaagcgac cggtcgcctc tttccgcgct
      541 gacagtggag tgggcgacag gtgatttcgc caaccctgaa acattcaccg ccgccctgcg
      601 cggcgcggac gctcttgtca acatcgcatc gctcggcttc gggcacgcag aatccattct
      661 aaagtccatg aaagaggcgg gcgtgaagcg cggacttttc atcagcacca ccgccatctt
      721 cacccagctg aacgcggtca gcaaatccat ccgccttgcc gccgaagaag ctatccaaac
      781 cagcggattg gactacacca tcctgcgccc taccatgatc tatggcagta aacgtgaccg
      841 caacatgtgg cggctgattc gtttgttgcg catcacgccc attatgccca tcttcggtga
      901 cggtgaatct ttgcagcagc ccatttttgt agatgatgtt gctcaagcgg tactgctcgc
      961 tttgaagaac gatatgacca ttggtaaaag ctataacatc gccggaaaag ctccgttgag
     1021 ctataaccaa gtcattgaca cggttgcttc tgcgcttggc aaacgtgtgt ggaagttgca
     1081 catgccctac atgaccatcg ttcgtgctct gcaattcacc gagcggatga aactgcgctt
     1141 gccgatcaaa gctgaacaag tgcttcgttt gaacgagaat aaagcgttct cctatgagga
     1201 agctcaaaaa gattttgatt tcaactcgcg tagttttgaa gaggggattc gaacggaaat
     1261 acaggcaaat aaataaagtg gattattcta ttctataagc gaatagaaga gggggaatta
     1321 gctaaaacgt aggtggggaa tccccccatg ttcatgccgt cttgcgtaag tgataatgta
     1381 acaaattatg ttggtattgt gctaaaaaga aggctttgat caatattcta tatagttgat
     1441 gaagaatatc atgataatta tggaattatt gtagtagtga taaaagggaa actgacaaat
     1501 aatagccttc tagatttaga aaaaatgccc atcgtgcctg gagatattta tgcccaaaaa
     1561 taaaaccaag atggatattc aagctgaatt ggaagcggct caaaagcgag tggctgagct
     1621 tgaagaagcg ttcgggagtg atcgggttga acagcgtttt gccagtgtgt ttcgagcaag
     1681 cccatctcaa atggcactga cggatcagac cactggaagg tatatcgaag ttaacgaagc
     1741 ttttttgaat accttgggat tttcgcgaga agaggtactt ggtaagactg caactgagtt
     1801 gaacttgttt gtagacccgc aacaaaggca ggatctgctc ttgcggatgc gagaccaagg
     1861 attcctaagg aatgaacatg tacttgtgcg tgcaaaaacg ggggagatca gacatggtat
     1921 tttttcagca gaatttataa atacaactga acaaaaattg cttcttactg ttatgaacga
     1981 tgtgacagaa aaattgcagg cggagcaacg ctggcaattt gcattagatg gtgcagatga
     2041 aggggtatgg gattgggatc cacagaccaa caaagtcttt tattcaaaac gttggaagtc
     2101 tatgctggga tatgaagacg gagaaattgg caatacgtta gaggaatggt ctagccgcgt
     2161 tcaccgcgat gatttgccca gagtaatgca agaggtgaca agacaccttt ctggagagac
     2221 accgatttat aagagtgagc atcgtatcct tcgtaaggac ggaacctatt tgtgggtatt
     2281 ggatagtggc aaagtaatgg agtggtctga agataaaaaa ccgcttcgtg tgatcggaac
     2341 ccataaagat atatctgagc gaaaaaaagc agaggaatca ttacgagaaa atgaagagcg
     2401 tttttcacgt atgttccatg ccaaccccgc tgtacaactg attgttaagt ttgatgatgg
     2461 cattgtttta gatgtaaacg aatcgttctg cctgcaaact ggttttgacc gtgatgagtt
     2521 gatcggtcgt tctatgcgtg acttaaattt atggcaggat ccgtttaaac aaaagcgcat
     2581 catggacgaa ctgcgttcta aaggtggagt gcatgatgtt gaacttgaat tctggaccaa
     2641 caccggtgac cttcgtactt tactcatttc atttgacctt gttgaaatta gaggggtatt
     2701 gtgcaacctc gccacaggtg tggatattac ttcacgcaaa caagccgagc gggatttgcg
     2761 ttttagagag aaacaatatc attcacttgt tgaaacgctg gatgtcagta tctgtcgttg
     2821 gctgccggat acaacattga catatgccaa tgaaaagtac agaaaaatat ttaatattac
     2881 gggaaatgcc gaaggtaaaa aatggattga tcttttacca gaggcggcac gtgcttctac
     2941 tgccattttt tatagtgagg tgactaaaca ccctcgcaca gtgatgtatg atcacccggt
     3001 tacgatgaac gatgggaaaa tccatcatta tcattgggtg gatactccaa ttttagggaa
     3061 tgatggtgaa gtacttgaat ttcaatcgat tggcatcgat attacggatc gaaagctctc
     3121 agaaaatgca ttagttgcca gtgaaaagag attgaaatct ttggtggatt ctcaatctca
     3181 ttatgtgatc cgcgtaaata tggagggaag atattcctat tggaatgcgc agtttgagaa
     3241 agaatttggg tggctatatg aatctgaagg gatacagaat gcctttgtgc ttaaagcagt
     3301 ttgtagttat caccatgagc gagtgacaaa tattgtcaat caatgtattg ctgagcccgg
     3361 aaaagtattc actgttgaaa ttgataaacc tgcccgaaac ggtagtatcc gcactacatt
     3421 gtgggagttc atttgtctta ccgatgaatt agaccaacct tatgaaattc aatgcatggg
     3481 cgttgaaatt acggggcgta aacaagcaga agatgattta cgtaaaaatg agcaaatgct
     3541 tcgcctcttc attgagcata gcccagctgc cattgccatg tttgaccttg agatgcgtta
     3601 tatcattgcc agtaaacgat atctcattga ttatcgcctt ggcgatgtag atttggtggg
     3661 acgttctcac tatgagattt tcccggagat gaccgaagag catcggcata tccatcagcg
     3721 ctgtttgatg ggcgagatcg ataagaaaga tgaagattta cttgtccgta acgatggcac
     3781 ctctgattgg gtgcgttatg aacttcgccc ttggcatcaa tc
//
LOCUS       DAOREF010000177         3811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14679_length_3811_cov_4.131789, whole genome shotgun sequence.
ACCESSION   DAOREF010000177 DAOREF010000000
VERSION     DAOREF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14679_length_3811_cov_4.131789"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKI33_04940"
     CDS             complement(<1..815)
                     /locus_tag="PKI33_04940"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNM36382.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAAVKTAINEYQCEVIGIRDGYDG
                     FLDTQNGIVPLGMEEVRGILPRGGTILGAANRGNPYARKVIRDGKEVTIDVSDEIVKG
                     IKELGLDALLVLGGDGTLHIAHELAQKGVPVIGVPKTIDNDIGGTEITFGFDTALNIA
                     TEALDRLHTTAESHHRVMVVELMGRDAGFITLNAGISGGADVILIPEIPFKFENIMKK
                     IQSRSGQGRKFSLIAVSEGAKPIGGMQVYSRKGDEVYVPRLGGIGQVVAEFIE"
     gene            906..1754
                     /locus_tag="PKI33_04945"
     CDS             906..1754
                     /locus_tag="PKI33_04945"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNM36383.1"
                     /translation="MQTNPQPSKLETIASWVVPIAAIAAVIAWFIIAPEGALGKLDAI
                     GYAVCHRIDVRSFHIGDRQLPLCARCTGEFYAAGLTLLFQMVVSGKRSKLPSRGVMAV
                     LFGFFVAFGVDGTNSYLYLLKETGRNSGFLAQIPNLYVPNNVLRLFTGSGMGIALAAM
                     LYPVINQTLWRTLDDRPAIEWKQLGILVAGIIGINLLVLTDSPFVLYPIAYISALGTL
                     SLLVIVFGLLWLIIMKQDNSFEHPRQLWLAFASGLTLALLLILSIDLMRLNFTGTWSG
                     FPGLSG"
     gene            1814..2386
                     /locus_tag="PKI33_04950"
     CDS             1814..2386
                     /locus_tag="PKI33_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNM36384.1"
                     /translation="MELLLGIFTAFGLSASAGLNAYIPLLVVGVIAHYAPNTLNLASP
                     FDLLANPWILILLGVLVIIEMVADKVPAVNHINDLIQTIIRPVAGAIAFAASANVITD
                     VNPVLALACGLLVAGSVHTVKAAAVRPAVTATTAGAGNIPVSIAEDVIATITSVLAIL
                     IPLIAGTLFIIFLVLVLWWWASRKQKAQSA"
     gene            2433..2585
                     /locus_tag="PKI33_04955"
     CDS             2433..2585
                     /locus_tag="PKI33_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36385.1"
                     /translation="MSEEMPVAPSETPAPNNNNRNIIIGVVAVVVLCCCCVLGSGIGY
                     YVWNNF"
     gene            complement(2608..3057)
                     /locus_tag="PKI33_04960"
     CDS             complement(2608..3057)
                     /locus_tag="PKI33_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNM36386.1"
                     /translation="MKILKKNLADEVTWQYEGEELSRDKNSITIEALFNRDDMPFQEI
                     VLKRNDRFVETFYSDKWYNIFAIYDRDDGSLKGWYCNITKPAIIEIDSVSYVDLALDL
                     WVSANGKQTVLDEDELEELKLGEALKENVYAGLRELQAFFNQKNPLP"
     gene            complement(3054..>3811)
                     /locus_tag="PKI33_04965"
     CDS             complement(3054..>3811)
                     /locus_tag="PKI33_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36387.1"
                     /translation="LVFQDYGLFPHLNVFDNIAFGLKMRNVPTEEINQRVTALIEQIN
                     LSGFETRSIIDLSGGEQQRVALARALAPQPRLLMFDEPLGALDRSLKEDLLNEIRGIL
                     HQTKIPAIYVTHDQEEAFTIADRILVLHEGTIIRDAAPHEIWREPKSAFVAKLLGIGN
                     VIDGEVVADKKVKTAFGVFALDCKKQVGEKAQLLMTVGEGRGEEIQLTVEDVLFKQDQ
                     FQVKARGGIVIHMKEAPKVGDIINIKVQVKCLS"
BASE COUNT          915 a         1073 c          804 g         1019 t
ORIGIN      
        1 tgttcaatga attcggcaac cacctgcccg atccctccca agcgcggcac atacacctca
       61 tcccctttgc gcgaatagac ttgcatgcca ccgataggct tcgcgccttc agaaaccgcg
      121 atcaagctga acttccgtcc ctgcccactg cgagactgaa tctttttcat gatgttctca
      181 aacttgaatg ggatctcagg aatcaaaatc acatctgccc cgcctgaaat acctgcattc
      241 aacgtgataa acccggcatc gcgccccatc aactccacca ccatcacgcg gtgatgcgac
      301 tctgccgtag tatgcaaccg atccaatgct tcagtagcga tattcaaagc agtatcaaac
      361 ccaaaggtga tctctgttcc gccaatgtcg ttatcaatcg tcttgggcac accgatcacc
      421 ggcactcctt tttgagccag ttcatgtgca atatgcaaag tgccgtctcc accaagcaca
      481 agcaatgcgt ccaagcccaa ttctttaatg cctttcacga tttcatccga cacatcaatc
      541 gtgacttctt tgccatctcg aattaccttg cgagcatacg ggttaccacg attcgccgcc
      601 ccaagaatcg tcccaccacg tggaaggatt ccgcgcacct cttccatacc caaaggcaca
      661 atcccgtttt gtgtatcgag aaaaccgtca tacccatcac ggatgccgat tacctcgcac
      721 tgatattcat tgatcgccgt ctttaccgcc gcacggatga ccgcattcaa ccccggcgca
      781 tcgccgccgc ctgtaaggat tccaattcgt ttcatgatgt cacctctata tagaagatga
      841 taacgcaaaa tattaaatat tcctcaaaag agagattttc acatcaggct gggtataatc
      901 tcaaaatgca aacaaatcca caaccctcaa aacttgaaac cattgccagt tgggtcgttc
      961 ccattgccgc aattgcagcc gtaatcgcct ggtttattat cgcgcctgaa ggcgccttgg
     1021 gtaagctcga tgccattggc tatgctgtct gtcatcgcat tgacgtacgt tccttccaca
     1081 ttggcgaccg tcaactcccc ctatgtgccc gctgtaccgg tgaattctat gccgccgggc
     1141 ttaccctgct ttttcaaatg gtcgtaagtg gaaaacgcag caagctccct tcgcgcggag
     1201 tcatggcggt gctctttggt ttcttcgtcg cctttggcgt ggatggcacc aattcttacc
     1261 tgtacttgtt aaaagaaacc ggcagaaact ctggttttct ggcgcaaatt ccaaatctat
     1321 atgtgcccaa taatgtcttg cgcctcttta cgggcagcgg catgggcatc gccctcgctg
     1381 ccatgctcta cccggttatc aatcaaaccc tctggcgcac cttggatgat cgccccgcca
     1441 tcgaatggaa acaactcggg attctggtag cgggcatcat cggcatcaac ttacttgttc
     1501 ttaccgatag tcccttcgtg ttgtacccca ttgcttatat cagcgccttg ggcactcttt
     1561 cgctgttggt gatcgtcttc ggattgctat ggctcatcat catgaagcaa gacaactcct
     1621 tcgaacatcc acgtcagctt tggctggcgt tcgcctccgg cttgacccta gcattgttgc
     1681 tcatcttgag cattgaccta atgcgcctca acttcacagg cacatggagc gggttccccg
     1741 gtctatcagg ctaacaacga atatactcaa aaatcgtata atcaagttgt acgatctttg
     1801 ataaggagaa gaaatggaac ttttacttgg aatttttact gcctttggtc tttcagccag
     1861 cgccgggttg aatgcctaca ttccactttt ggtggtgggt gtcatcgctc attacgctcc
     1921 caacaccctc aacctcgcct cccctttcga cctgctcgcc aacccgtgga tcctcatcct
     1981 actcggcgtg ttggtcatca ttgaaatggt ggcagataaa gtacccgcgg tcaatcacat
     2041 caacgacctg atccaaacca tcatccgccc ggtcgcaggt gccatcgcct tcgccgccag
     2101 tgccaatgtc atcaccgatg tcaaccccgt gctagcactt gcctgcggtt tgctggtagc
     2161 tggcagtgtt cacaccgtaa aggcggcagc cgtgcgccca gcggtcacag ccacaacagc
     2221 aggggcgggc aatatcccag tttcgatcgc tgaagatgtg attgcaacca tcacttctgt
     2281 tctagcgatt cttattccgc taattgctgg aactttgttc attatcttcc tagtgcttgt
     2341 cctgtggtgg tgggcaagcc gcaaacaaaa ggcacagtct gcctgacatc gtttataatc
     2401 caaaaaaata actttctaaa aggagagaaa tcatgtctga agaaatgccc gtcgccccat
     2461 ccgaaacccc ggcgcctaac aataacaatc gcaatatcat catcggtgtc gttgccgttg
     2521 tcgtgttgtg ctgttgctgt gtgcttggct ccggtatcgg ttactacgtc tggaataatt
     2581 tctaatttcc aacaaaaaat cccctgctca tggcagggga tttttttgat taaagaacgc
     2641 ttgcaattca cgcaacccgg catatacatt ttctttcaat gcctcgccca acttcaattc
     2701 ttccaattca tcttcatcca aaacggtctg ttttccattt gcagagaccc aaagatcaag
     2761 cgcaaggtca acgtatgaaa ccgagtcaat ttcaataata gcgggcttgg taatattaca
     2821 gtaccagcct ttcaggctgc catcatcacg gtcgtaaatg gcaaaaatgt tgtaccattt
     2881 atcagaataa aaagtttcca caaagcggtc gttgcgtttt agtacgatct cttgaaacgg
     2941 catatcatca cgattaaaca acgcttcaat cgtgatcgaa ttcttatcac gactcaactc
     3001 ctcgccctca tactgccaag tcacttcatc agctagattc tttttcagga ttttcatgac
     3061 aggcatttta cctgaacctt gatattgatg atatcgccaa cttttggcgc ttctttcatg
     3121 tggatgacga tcccacctct tgccttgacc tggaattggt cctgcttaaa gagcacatcc
     3181 tccactgtga gttgaatctc ctctcccctt ccctccccaa cggtcatcag caattgagct
     3241 ttttctccaa cttgtttttt acagtcaagt gcaaaaacgc caaacgctgt tttaactttt
     3301 ttatccgcaa ccacttcccc atcaataacg ttcccgattc ccagcagctt cgccacaaaa
     3361 gcagacttgg gctcgcgcca aatctcgtgc ggagcagcgt cgcgaatgat ggtgccttcg
     3421 tgcaaaacaa ggatgcggtc tgcgatggtg aaggcttctt cctggtcgtg ggtgacgtag
     3481 atggcgggga ttttcgtctg gtgcaagatg ccgcgaattt cgttgagcag gtcctccttc
     3541 agtgacctgt ccaatgcgcc gaggggttca tcgaacatga gtaatcgagg ttgtggcgca
     3601 agcgcacgcg caagagccac gcgttgttgc tctccgccgg agagatcaat tatgctgcgt
     3661 gtttcaaagc ctgaaagatt gatttgttca attaaagcag taacacgttg gttaatttct
     3721 tcagtaggta cattacgcat cttcagccca aaggcgatgt tgtcaaaaac gttcaggtga
     3781 gggaaaagcc catagtcttg gaagaccaag c
//
LOCUS       DAOREF010000178         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14697_length_3809_cov_4.134523, whole genome shotgun sequence.
ACCESSION   DAOREF010000178 DAOREF010000000
VERSION     DAOREF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14697_length_3809_cov_4.134523"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKI33_04970"
     CDS             complement(<1..233)
                     /locus_tag="PKI33_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HNM36388.1"
                     /translation="MDQLLLILTYIAVIASSVSGALGARKYQMDIVGAVTIAYVNAFG
                     GGTVRDLLLGRTPIFWIADPALTVTTLIVAIIS"
     gene            complement(308..2806)
                     /locus_tag="PKI33_04975"
     CDS             complement(308..2806)
                     /locus_tag="PKI33_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNM36389.1"
                     /translation="MNEFDKNKKLETLLEETAEQLQPNVMFKAELEEKLRKAHKPRAS
                     LPTFAFRNWLPTLTGIVVVGGLVIFMLWLFQTLEPQKSVGTNEEQPIEVQTTPGVIID
                     PNNTNSNETIGGYDYRGGKLFLTSALPESSATANIYEYIADQPATIEEARALANQFGL
                     QGEVYTTAFPQAPDKIGYVISDGKRMLTVYTKNYFTYTPDILVTDRGYYGVNNDNAET
                     VISDFFNQNGLTFPFSISKSPAKGMYHFMQLSPDGIPMQYDIYTIQSSNVTLGQNGEV
                     AYLSANMLSYNPQAFGSYGIISAEDALNAILDPHSTVGMTESGSGGEGGVIPQQWYRE
                     YPNNETVSISGSLIKYDSATAGQPPLIFVGSFEALGNTTDLQALSNFAFVQVTGQFVE
                     ENGIRKFNVESTNANVIQGYITGSLRKEGDQIILTSDDGNSEYVLLDPPADLPLNTVP
                     TESYLGISGILVDNTLDWSNIQYVADASSMGGGGGGGGFGFYPLNLSGTPAVFPTPIP
                     AEEQYSAAEVASFLNYIVENGDTLESIAAKFNVSLEDLMRVNYISDMNVIETGWLIKI
                     PGVPGPTQLTGEEGIVSISKYIKPDGRVRYQYTFLSSQSYNGIYYELQGDNLEPLQNL
                     VNRPVKIWGDISYSDTGTMFLDVKQFEEIYPGLQFQILQGKEALTEINGKPVALFTTN
                     GTTYVMLSEVGAYQDISYKGDFPEDINIEALIVPGETYEGMPALRVFSMANAKDPNTG
                     EAIELEPFAGEIQSSPDPFGNSDVYVEPDFIVDYIQLEYYVTDPKMLYDAPTTDTLYI
                     QPVWHFSGHYTNGTVSDILVQALKQEYLYPFNSP"
     gene            complement(2803..3375)
                     /locus_tag="PKI33_04980"
     CDS             complement(2803..3375)
                     /locus_tag="PKI33_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNM36390.1"
                     /translation="MTTLINMEIKELDEEDLARKAISDADAFAELYRQHVTRVYRYHM
                     AHIGNVKDAEDLTSQTFMAAMESIRSFRGDGSFAAWVMGIASKKRLMYFRGNRHEVPL
                     EAAEHYPNPSLPTDKAAMQRIRLQSISHALKQISSERSEAIVLIYFGGLSYAEAAGVM
                     RKNEASVKMLVSRGLQDLRERTSLRMEVEE"
     gene            complement(3480..>3809)
                     /locus_tag="PKI33_04985"
     CDS             complement(3480..>3809)
                     /locus_tag="PKI33_04985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNM36391.1"
                     /translation="GVLHGGIVAAIIDEVSGRAYMGSDPANPRFMFTGKLEIKYRKNV
                     PIGKLLRIVGKVMENKGRIAKAWGGIYDAETNELLAEGTGMHINVPSDQFDMSKLEEL
                     GWKIYPD"
BASE COUNT          924 a          895 c          913 g         1077 t
ORIGIN      
        1 aaggagataa tagcgacgat taacgtagtg acggttaatg cagggtcggc gatccagaag
       61 atgggcgtgc gccccagcaa taagtcgcgg acggtacccc cgccaaaggc gttgacatag
      121 gcaatggtca ctgcgcctac aatgtccatt tgatatttac gtgcgcctag cgctcctgaa
      181 accgatgatg cgatgacggc gatgtaagtg agaatgagaa gaagttgatc cattcgccaa
      241 gtatcacaca acttgtatta aaaagtccac catcatgtga tggtggactt ttttgttgct
      301 ttactggtta cggtgaattg aaggggtaga gatattcttg tttgagcgct tggacgagaa
      361 tatcagagac ggtgccgttt gtgtaatgtc cgctaaagtg ccagacgggt tgaatgtaca
      421 acgtgtctgt ggtgggagcg tcgtaaagca tcttggggtc ggtaacatag tattcgagtt
      481 ggatgtaatc aacgatgaaa tcaggttcga catatacatc tgagttcccg aatggatctg
      541 gagatgattg tatctcgcca gcaaatggtt ctaattcaat agcctctccg gtgttcgggt
      601 ctttggcatt tgccatactg aataccctca aggcgggcat gccttcgtat gtttcacccg
      661 gcacgatcag agcttcaatg ttgatatctt cagggaaatc acccttataa ctaatatctt
      721 gataagcacc tacttctgag agcatcacat aggtggtgcc attggttgta aagagcgcaa
      781 cgggttttcc attgatctcg gtaagcgctt ctttgccttg cagtatctgg aattgcaacc
      841 ccgggtagat ttcttcgaac tgttttacgt ccaagaacat cgtccctgta tctgagtagc
      901 tgatatcgcc ccaaatctta accgggcggt taaccaagtt ttgaagtggc tcaagattat
      961 cgccttgcaa ttcgtaatag atgccattgt acgactgtga gcttaagaag gtgtattgat
     1021 accgcactct gccatctggt ttgatgtact tcgagatgct tacaatacct tcttcgcctg
     1081 ttagttgagt aggacctggc acgcctggaa ttttgatgag ccagcctgtt tcaatgacat
     1141 tcatatctga aatgtagttc acgcgcatga ggtcttccaa cgagacgttg aatttggcag
     1201 caatgctttc caaggtgtca ccattctcta caatatagtt caagaaacta gcgacttctg
     1261 cggcgctata ttgttcttct gctggaattg gtgttgggaa aacagcagga gtgccgctta
     1321 agttgagcgg atagaaacca aacccgccgc ctccgccacc tccgcccatg ctactggcat
     1381 ctgcaacata ttggatgttt gaccagtcca gagtgttatc gaccaaaatt ccagagatac
     1441 cgaggtatga ctccgtaggc accgtattaa ggggcaagtc tgcgggtggg tctagcaaaa
     1501 catactcgct gttgccatcg tcgctggtaa ggatgatctg atcgccttcc ttgcgcagcg
     1561 agccggttat atatccctga ataacattgg cgttcgtgct ttccacatta aatttgcgga
     1621 tgccattttc ctcaacaaat tgacccgtca cttgtacaaa ggcaaagttg ctcagagctt
     1681 gtaagtctgt ggtgtttcca agtgcctcaa aactgccaac aaaaatcaac ggtggctgac
     1741 ctgctgtcgc agagtcgtat tttatgaggc tgccagagat tgaaacggtc tcattatttg
     1801 gatattctcg ataccattgt tgtggaatca cgccgccttc tccgccagag ccgctttcgg
     1861 tcatgccgac ggtgctatgt gggtcgagga tggcattcaa ggcatcttcc gcgctgatga
     1921 tgccatagct tccaaatgct tgtgggttgt agctgagcat atttgcagag agataagcta
     1981 cttcaccatt ttgtccaagc gtgacatttg aactttggat ggtgtagatg tcatattgca
     2041 tggggatgcc atctggcgag agctgcatga aatgatacat accctttgca ggagacttgc
     2101 tgatgctgaa tgggaacgtt aacccgttct gattgaagaa atcagaaatg accgtctctg
     2161 cattgtcgtt attcacacca tagtaaccgc ggtctgttac caaaatatcg ggtgtgtatg
     2221 tgaagtaatt tttggtatac acagtaagca ttcgcttgcc atctgaaatg acataaccaa
     2281 tcttatcggg cgcttgtgga aatgcggttg tatacacttc accttgtaac ccaaattgat
     2341 ttgcaagcgc tcgcgcctct tcaatggtag cgggctgatc agcaatgtac tcatagatat
     2401 tggcagttgc tgaactctca ggcaaagcag atgtaaggaa caacttcccg ccgcgatagt
     2461 catatccgcc aatggtttcg ttgctatttg tattattcgg gtcaatgatg acgcccggag
     2521 ttgtttgcac ttcaataggt tgttcttcgt tagtgccaac gcttttttgt ggctccaacg
     2581 tttggaagag ccaaagcatg aaaattacca atccgccaac aacgacaata cctgtcaacg
     2641 tgggcaacca gtttcgaaat gcaaaggttg gcaaacttgc gcgtggctta tgtgccttac
     2701 gaagcttctc ttccaattcg gctttaaaca ttacattggg ttgtagctgc tcggcagttt
     2761 cttccaataa cgtttcgagt tttttgtttt tatcaaattc gttcattctt ccacctccat
     2821 tctgagggag gttcgctcgc gcaggtcttg cagcccgcgt gagacgagca ttttgacaga
     2881 cgcctcattt tttctcatca cgcctgcggc ttcggcgtag cttaatccgc caaagtagat
     2941 aagcacgatc gcttcggagc gttcacttga aatttgcttg agcgcatgag aaatagactg
     3001 gagccggatg cgttgcattg cggctttatc ggtaggtaag ctcgggttgg gataatgctc
     3061 cgccgcctct aacgggacct cgtgcctgtt cccgcgaaaa tacataagtc tcttctttga
     3121 agcgattccc atcacccacg ccgcaaacga gccatctcct cgaaacgagc ggatactttc
     3181 catggctgcc atgaaggtct gcgaggttaa gtcttcggca tcttttacgt tgccgatatg
     3241 tgccatgtga tagcggtaca cccgcgtcac atgctgacgg taaagctctg caaaagcatc
     3301 tgcatctgag atggctttcc gtgctaggtc ttcttcgtct agttctttga tttccatatt
     3361 tattagggtg gtcatatacc tcctgtgagt agtgcgcaca tagatatgtt gcaggaggtt
     3421 gaaaaaagta acaggcaaaa taaaaggtga cagtcacttt ataagcgact gtcacctggc
     3481 tagtcagggt atatcttcca acctaactct tcaagcttgc tcatatcgaa ctggtcactt
     3541 ggcacattga tgtgcatgcc tgtcccttcg gcaagcagtt cattcgtctc tgcatcgtaa
     3601 attccgcccc aggctttggc aattcgcccc ttattctcca ttactttacc aacgatcctg
     3661 agcaatttgc caatcggcac attcttgcgg tatttgatct ctaacttgcc cgtaaacata
     3721 aaacgcggat tcgccggatc actgcccatg tatgcccgcc cagagacttc gtctatgatc
     3781 gcggcgacga tgcccccatg caacacacc
//
LOCUS       DAOREF010000179         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14771_length_3796_cov_3.169206, whole genome shotgun sequence.
ACCESSION   DAOREF010000179 DAOREF010000000
VERSION     DAOREF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14771_length_3796_cov_3.169206"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="ltaE"
                     /locus_tag="PKI33_04990"
     CDS             complement(<1..776)
                     /gene="ltaE"
                     /locus_tag="PKI33_04990"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNM36392.1"
                     /translation="MNYVDLRSDTVTKPTPEMREAMAEAEVGDDVYGDDPTVNQLQVK
                     AAEMLGKEAALFVPSGTMGNLLALLVHCSRGEEVICGDKSHIYVNEAGGMAALGGIYP
                     HPVPNQKDGTLRLEDIRASIQPDDSHRTITRLVCIENTQNMCGGVVLSVEYTKQVGQL
                     AKEHGLKFHIDGARIFNAAAALNVDIKELVAPADSVMFCLSKGLVAPIGSMLVGTKEF
                     IAKAHRLRKMLGGGMRQVGVMAAAGLVSLDVMTGRLKQDHA"
     gene            complement(809..1735)
                     /locus_tag="PKI33_04995"
     CDS             complement(809..1735)
                     /locus_tag="PKI33_04995"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNM36393.1"
                     /translation="MTDRIETFHYDEQNPDRLDKYLVSCLPEFSRARIQGLILDGFVS
                     INGVPAKKAGQVIEDGDDIEVRVPPPVPSGLVAEDIPLDIIFENDDLIVVNKPAGMVV
                     HPAAGHYSGTLVNAVLGYDPDLEGIGGEERPGLVHRLDKETSGLIILAKNERAHNWLQ
                     DQFRLRKVEKTYLALVDGKPPTPAGRVEAPIGRDPSHRKKMAIVSPGKGREAVSEYKT
                     LESFRHHTLLEFHPHTGRTHQIRLHCQFLGCPIVGDSVYGKRTNTATIDRHFLHAFRL
                     KIILPNEKQPRMFEAELPEELKAVLEEVKRYE"
     gene            complement(1732..2277)
                     /gene="lspA"
                     /locus_tag="PKI33_05000"
     CDS             complement(1732..2277)
                     /gene="lspA"
                     /locus_tag="PKI33_05000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNM36394.1"
                     /translation="MTEEISVQEKTQPRSRRFFIFFAVAIVIIALDQWTKWLVIQSVP
                     LNTSWLPEALDWLSPYARLLHIQNRGVSFGMFQGGNTVFIILTTVIVAAIVFYVSRMD
                     HPSDWMWVAAGLYLGGAVGNLIDRLTIGAVTDFISVGNFYIFNIADASINISVVLLLL
                     LSWLHERRSSTLETEPKDVSA"
     gene            complement(2282..2821)
                     /gene="lspA"
                     /locus_tag="PKI33_05005"
     CDS             complement(2282..2821)
                     /gene="lspA"
                     /locus_tag="PKI33_05005"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNM36395.1"
                     /translation="MNSKLRDYLMLFGVAGVSVALDQWTKWLVRTNIEYSGQWLPDWL
                     NWLSPYARIVHWYNSGAAFGMFQNGNLIFTILAFVVIAAILYYFPSVDSDDWTLKLAM
                     GLQLGGAVGNLIDRLIMQKVTDFISIWTFPVFNIADASISVGVAVLLLGVWLKEREEK
                     LKAAQVKTEAVPETMKEGS"
     gene            complement(2848..>3796)
                     /gene="alr"
                     /locus_tag="PKI33_05010"
     CDS             complement(2848..>3796)
                     /gene="alr"
                     /locus_tag="PKI33_05010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNM36396.1"
                     /translation="VEEGIQLRQLGIKKPILVAGGTLIEQLPLFFEHDLTITASSLDL
                     LLAANQLAESTRKRLTVHLKIDTGMERVGVREYEAEEFILKEFACSHLNVEGIYTHLA
                     NSELVELTGMPSHVVTAQEQLERFQEVLHLYEKHSLPVPSIRHVCNSGGILNLPDGHL
                     DMVRPGVLFYGVYPGRDIPRTIDVKPALTWKSQVSYSKVTQPGRAVSYGSLWQVEGSP
                     KRVATIPCGYADGYFRRMTNQGRVLINGKSYSQVGRICMDQFMVDAGSDEIVIGDEVI
                     LLGSSIAPEELADWAGTNEYEVMTNISARVPRVFVGGVQ"
BASE COUNT         1030 a         1085 c          768 g          913 t
ORIGIN      
        1 gcatgatcct gtttaagccg ccccgtcatc acatccagtg acaccaatcc tgccgccgcc
       61 atcacaccga cctgacgcat tcccccaccc aacatcttgc gcaagcgatg cgccttggca
      121 ataaactcct tcgtaccaac caacatcgac ccaattggcg caaccaaacc cttgctcaaa
      181 cagaacatca ccgaatcagc tggggcaaca agctctttga tatctacatt caaagcagca
      241 gccgcgttga aaatacgagc accatcaata tggaatttca atccgtgttc ttttgcgagt
      301 tgaccaacct gctttgtgta ctccaccgaa agaaccacac ccccgcacat gttctgcgtg
      361 ttctcgatgc aaaccaaacg cgtaatggtg cgatgtgagt catcgggttg aatcgaggcg
      421 cggatgtctt caagacgaag tgttccatcc ttttgattcg gcacgggatg aggataaatc
      481 ccgcccaacg ccgccatccc acctgcctca ttcacataaa tatgcgactt atcgccacag
      541 atcacttctt cgccgcgcga acaatgcaca agcaacgcca acagattgcc catcgtgccc
      601 gaagggacga acagagccgc ctccttacca agcatctctg ccgccttcac ctgcaattga
      661 ttcaccgttg ggtcatcccc gtaaacatca tcccccacct ccgcttctgc cattgcctca
      721 cgcatttcgg gcgtcggttt ggttacagta tcagatcgta aatctacata gttcatggaa
      781 atctccgttt attcaagtag ggtgctactt actcatatct ctttacttct tccaagacag
      841 ctttcaattc ctctggtaat tccgcttcaa acatgcgggg ctgtttttcg ttcggaagga
      901 taatcttcaa ccgaaacgca tgcaagaaat gccggtcaat ggtagcagtg ttagttcgct
      961 tcccataaac tgagtctccc acgatcgggc aacccaagaa ttgacaatgc aaacggatct
     1021 gatgtgttct acccgtatgt ggatgaaact ccaacagagt atgatgccta aaagactcta
     1081 gcgtcttgta ttcactgacc gcttcacgtc ctttacccgg tgaaactatc gccatctttt
     1141 tgcgatgact tgggtcacgc ccaatcggcg cttctacacg cccagccggt gttggtggct
     1201 tgccatctac aagcgcaaga taggtctttt ccacttttcg caagcggaac tggtcttgca
     1261 gccagttgtg agcacgttca ttcttggcaa ggatgatcaa gcctgaagtc tctttatcca
     1321 aacgatgtac caaaccgggg cgttcttcgc cgccaatacc ttcaaggtct gggtcatacc
     1381 ccaatactgc attgaccaag gttccagagt aatgccctgc cgccgggtgg acgaccatac
     1441 cggcaggttt gttcaccaca atcaggtcat cgttttcaaa aataatatcc agcggaatat
     1501 cttctgcgac caatccactt ggcactggcg gcggaactcg cacttcgata tcgtcgccat
     1561 cttcaattac ttgccctgct tttttagcag gcacaccatt gatcgataca aatccatcca
     1621 aaatcaagcc ctgaatacgg gcacgtgaaa actcaggcag acaggaaacg agatatttat
     1681 ccagccggtc tggattctgt tcgtcataat ggaatgtttc aattcggtcg gtcatgcgct
     1741 cacgtccttg ggctcagttt caagcgttga cgatcttcgt tcatgcaacc aagataaaag
     1801 caataacaaa acgacactga tgttgatact agcatctgca atattgaaga tgtaaaagtt
     1861 acccacggaa atgaaatccg ttacggctcc aatcgtgagg cggtcgatca agtttccaac
     1921 ggcaccgccc aaatacaagc cagccgccac ccacatccaa tcactggggt gatccatacg
     1981 agacacataa aagacaatcg ccgccacgat cacagtcgtc aagatgataa acaccgtatt
     2041 cccaccctgg aacatcccaa acgaaacacc gcgattctga atatggagca aacgggcata
     2101 tggcgaaagc caatccaacg cttcaggcaa ccaagatgtg ttgagaggga cagattgaat
     2161 caccagccat tttgtccact ggtcgagggc aatgatcacg attgccacgg caaaaaagat
     2221 aaaaaaacgc ctgctacgag gctgcgtttt ttcttgtaca ctgatttctt cagtcatcaa
     2281 actacgaacc ttctttcatt gtttctggca cggcttccgt ttttacctga gccgccttta
     2341 gtttctcttc tcgttcctta agccataccc ccaataataa aaccgctacc cccaccgaaa
     2401 tggatgcatc tgcaatatta aaaaccggga aggtccagat cgaaataaag tctgtcacct
     2461 tttgcatgat caaacggtcg attaaatttc caactgcgcc gcccaattgc aaccccatcg
     2521 ccaatttcaa cgtccaatca tctgagtcaa cactgggaaa gtaataaaga atggcagcaa
     2581 tcacaacaaa tgccaaaata gtgaaaatca agtttccatt ttgaaacatg ccaaacgcag
     2641 cgccgctgtt ataccaatgc acaatacgtg cataagggct taaccaattt aaccaatcag
     2701 gtaaccactg cccactatat tcaatatttg tacgtaccag ccactttgtc cactgatcga
     2761 gtgcaaccga aacgccagcc acgccgaaca gcattaaata atctcttaac ttggaattca
     2821 actctcacct caaaacgaaa agtgcgccta ttgtacccca cccacaaata ccctcggcac
     2881 gcgtgcgctg atgttggtca tcacctcata ttcgttggtg cctgcccaat ctgccaactc
     2941 ttctggcgca atgctgctgc ccaacaatat cacctcatcc cctatcacta tttcatcgct
     3001 cccagcatcc accataaact gatccatgca aatacgacct acttgcgaat acgacttgcc
     3061 gttgatgagg actcgtccct gattcgtcat gcggcggaag tagccatcag catatccgca
     3121 tgggatggtg gccacgcgct tcggactccc ttcaacctgc cacaacgatc cataactgac
     3181 agcccgcccc ggctgtgtga ccttgctata cgagacttgt gatttccaag tgagtgctgg
     3241 tttcacgtca attgtccgtg gaatatcgcg ccccgggtaa actccgtaaa acaagacacc
     3301 aggacgcacc atatccaaat gcccatcagg tagattcaga atcccaccag aattgcagac
     3361 gtgtcgaatc gatggcacag gcaaagagtg tttttcatat aagtgaagta cttcttgaaa
     3421 acgctcaagc tgttcttgag ccgtaacaac atgcgagggc atcccagtca actcaaccag
     3481 ctctgagttt gccaaatgag tataaatccc ctctacattt aaatgactac acgcaaactc
     3541 tttcagaatg aactcttccg cttcgtattc gcggacgccg acccgctcca tgccggtatc
     3601 aatcttgagg tggacggtga gtcgctttcg ggtagattcg gctaactggt tagcagccag
     3661 caacaagtcg agggatgaag cggttatggt taagtcatgt tcaaagaata aaggcagctg
     3721 ctcgatcaac gttccaccgg caacgagaat cggtttctta atcccgagct gacgaagttg
     3781 gatgccctcc tccacc
//
LOCUS       DAOREF010000180         3790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14811_length_3790_cov_3.583133, whole genome shotgun sequence.
ACCESSION   DAOREF010000180 DAOREF010000000
VERSION     DAOREF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14811_length_3790_cov_3.583133"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKI33_05015"
     CDS             complement(<1..819)
                     /locus_tag="PKI33_05015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNM36397.1"
                     /translation="MTKTIRNIIIVSLFTVGGGWLGIWLNNVTGNTMPPMQSLGALVW
                     LVSPALAGFALRTFGGDGWKDSGFGLHLLSSWKWYLLAILIYPLASLVTFGLGTLFGI
                     TSAEGLSTQGFSAYLSAVGVIFIGSLIKNFFEEFAWRSYLTPRLEAAGAHPIVNHVLV
                     GILWMSWHLPYYYYYLDRTVLESSITTSIPVFIALGYLVMIPTAILFGELRLISKSTW
                     VVFALHQVINAVSMPLLINGFIEVKGPLAPVFTPTNEGLFVSLLFGITGWLLMKK"
     gene            complement(821..1384)
                     /locus_tag="PKI33_05020"
     CDS             complement(821..1384)
                     /locus_tag="PKI33_05020"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNM36398.1"
                     /translation="MSLKHTILGFLSYTSLSGYDLKKAFDRSVQHFWPANQSQIYRTL
                     AELEEEGFVTKEVIEREERLDIKLYHITTDGRSELHQWLTHPLPERDDREPFLIQVYF
                     SGQLKDTEVLHLFRQKLRETEERLAVYESVYEMIQNTPKRIHDDRAIFFSVLTLEAGY
                     VNNKSIAEWLRSAIERIENKNYEINIG"
     gene            complement(1467..3548)
                     /locus_tag="PKI33_05025"
     CDS             complement(1467..3548)
                     /locus_tag="PKI33_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM36399.1"
                     /translation="MDINRPFMLALIIALGVSSVLTLAGFTAIGSWASLFASFITLSI
                     LIFKNNGIRDTAIMGLIVILIGAGLLAGKTGTLIIGGFIILEISIYGALETKGILVNK
                     FSDQNYFEDYLVLCMIVAMITALQWLVISRLKSIGVKAQKDLEERKKVQGQLEEAEAR
                     YRGLVESVPLVIYIAEPGVMGKWHFVSPQITKLTGFSPEDWVKQPGLWYSRIHPDDRD
                     RTIQDETEALREGKMPRLEYRLLTRDEQYIWISDESYVFVDTSKLLVQGFMLDITSRK
                     LAEEQLTKRVAELQAVHGISEKLVQRTDLQKLIFDTGEQIRQSLKANNVLIAIHDPNT
                     NLIHFPYDFEEGVPRKDVPIHFGEGMTTYIMEHKIPVIIKNEWDRRAGEMNVLNANSL
                     PAKSSVSVPIMTPERVIGVITIESTEHEYAFSESDSQLLMTIAANLAVAIEKTRLQES
                     VKKEMEIQEKLIRELEVKNEELERFTYTASHDLKSPLITIRGFLGYLEQDARAGKFER
                     LGSDIQRISDATEKMHRLLSELLDLSRVGRVASEKQNVPFEAIVTEALKRVEGQIKEK
                     QVEVQVRSGFPVVHVDKERLIEVIQNLVDNAVKFMGKQPSPHIEIGFNMQDGNPIFFV
                     RDNGIGIKKEFQKKIFGLFDKLNPATDGTGVGLALVKRIIEVHGGSIWVESQEGMGAT
                     FYFSLEKNNEN"
BASE COUNT         1049 a          861 c          791 g         1089 t
ORIGIN      
        1 ttttttcatc agtagccagc ctgtgatgcc gaataagagc gaaacaaaga gcccttcatt
       61 tgtaggcgta aagaccggcg cgagcggacc tttcacctcg ataaacccat taataagcaa
      121 aggcatactg accgcattaa tgacttgatg aagcgcaaaa acaacccatg tagatttgct
      181 aatgagacga agctcaccga ataatatagc agtgggtatc attaccaaat agcccagtgc
      241 gataaaaaca ggtatgcttg tagtgatgga actttccaac accgtgcggt ctaggtagta
      301 atagtagtag ggcaggtgcc aactcatcca taagatgccc acaaggacat ggttgactat
      361 tgggtgcgca cctgcggctt caaggcgcgg agttaagtaa ctgcgccagg caaactcctc
      421 aaagaaattt tttatcagag agcctataaa tattaccccg accgcagaaa gatatgcact
      481 aaagccttgg gtagacaagc cttcagcgga agtgatgcca aagagggttc ccaagccaaa
      541 ggtcacgaga gaggcgagcg gataaatcag aatcgctaac aagtaccact tccagcttga
      601 gaggaggtga agtccaaaac cagaatcctt ccagccatct ccaccgaagg tacgcagagc
      661 gaaccccgcc agggctgggc ttactagcca aacaagagcc cctaagcttt gcatgggtgg
      721 catggtattg ccagtgacgt tattaagcca gatgcctaac caacctccac ctacggtgaa
      781 gagcgaaaca ataataatgt tgcgaatagt ttttgtcatg ttaacctata ttaatttcgt
      841 agtttttgtt ctcgatacgc tcaatcgcag aacgtagcca ttcggcaatt gatttgttat
      901 tgacataacc ggcttcaagc gtaagtacgc tgaaaaagat ggcgcggtca tcatggatgc
      961 gtttcggagt gttttggatc atttcataga cactttcgta aacagccagg cgctcttcgg
     1021 tttcccttag cttttgccga aaaagatgaa ggacttctgt gtctttgagc tgaccactga
     1081 aatagacttg gatcagaaaa ggttcgcgat catcacgctc aggaagaggg tgagtgagcc
     1141 attggtgcaa ttcgctacgc ccatccgtcg tgatgtgata cagcttaata tccagccgct
     1201 cttctcgttc aatgacttct ttggttacaa aaccctcttc ttcgagctct gcaagtgtgc
     1261 ggtaaatctg actttggttt gcgggccaga agtgctgaac agaacggtcg aaggctttct
     1321 tgaggtcata tccagaaagg gaagtatagg agaggaatcc gaggattgtg tgttttaatg
     1381 acatatgcgt gtagtttata tagtacttgt attatatgat tagtactata tattattcca
     1441 agactcctgt caaggaaaag cctatgctag ttttcgttat ttttttcaag tgagaaataa
     1501 aatgttgccc ccatgccttc ctgcgattcc acccatatac tgccgccatg aacctcaata
     1561 attcgtttga ccaatgctaa accaacgcca gttccatctg tcgcggggtt gagcttgtca
     1621 aatagcccga aaattttctt ttgaaactct ttcttaatac ctatgccatt gtcacgcaca
     1681 aaaaaaatcg ggtttccatc ttgcatattg aaaccaattt caatatgtgg ggaaggttgt
     1741 ttgcccatga atttaacggc attatctaca aggttttgga tgacctcgat taacctttcc
     1801 ttatcaacat gaacgactgg aaatccgctt cttacttgga cctcgacctg cttctcttta
     1861 atttgacctt ctacacgctt tagggcttct gttacgatcg cctcaaaggg gacattttgt
     1921 ttttcactcg caacgcgccc tactcttgaa aggtcaagta attcactgag caaacggtgc
     1981 attttttctg tcgcatccga tatacgttga atatctgagc ctaatctctc aaattttcca
     2041 gctcgtgcat cctgttcgag gtaccctaaa aatccgcgaa tcgtgattaa tggagatttg
     2101 aggtcatgag aagcagtgta ggtaaagcgc tctaattcct cattttttac ttcaagctcg
     2161 cgaatgagct tctcttgtat ctccatttct tttttcacag actcttgcaa acgtgttttt
     2221 tcaatggcta cagccaggtt tgctgctatg gtcattaata actgcgagtc gctttcactg
     2281 aacgcatatt catgctctgt gctttctatt gtgataacgc caatcacgcg ttcaggggtc
     2341 ataatgggca cagaaacaga agattttgca ggtaaactat ttgcattaag aacattcatt
     2401 tcacctgcac gccgatccca ctcattcttg ataataactg ggattttgtg ttccataata
     2461 taagtcgtca taccctcacc gaaatggatg gggacgtcct tacgtggaac cccttcttca
     2521 aaatcataag ggaagtgaat taagtttgta ttggggtcgt ggatggcaat taacacattg
     2581 ttcgccttca atgactggcg aatttgttcc ccagtatcga aaataagttt ctgtaggtcc
     2641 gttctttgta ctaacttttc gctaataccg tgtacagctt gaagctctgc aacccgtttt
     2701 gttagttgct cttctgccag ttttcggctg gtgatatcta gcatgaaccc ttgtacaagt
     2761 aatttgctcg tatctacaaa gacataactt tcatctgaaa tccaaatata ttgttcatct
     2821 cgtgtaagaa gacgatattc gaggcgcggc attttgcctt cgcgtaatgc ttctgtttca
     2881 tcctgtatcg ttcggtcgcg gtcatccggg tggattcgcg agtaccaaag cccaggctgt
     2941 ttgacccaat cttctggaga gaaaccggtt agttttgtga tttgtgggct tacaaaatgc
     3001 catttcccca taaccccagg ctctgcaatg tagattacaa gtggcacgct ttcaacaagt
     3061 ccgcgatatc gggcttcggc ttcttctagt tgtccctgaa ccttttttcg ctcttctaga
     3121 tctttttgtg ctttgacacc tatgcttttt agccgactaa tgaccaacca ctgcaaagct
     3181 gtgatcattg caacaatcat acatagaact agatagtctt caaaataatt ttggtcacta
     3241 aacttgttaa caaggattcc ttttgtctca agtgccccat aaatactgat ttcgaggatg
     3301 ataaaccccc caataataag ggtaccagtt tttcctgcca ataaccctgc tccgatcaag
     3361 ataacaatca gccccataat ggctgtatct cgaataccat tgttcttaaa tatcagtatc
     3421 gagagagtta taaatgacgc aaacaacgat gcccaacttc caattgctgt aaaccctgct
     3481 agagtaagaa cgcttgagac cccaagcgct atgatcaacg ccagcataaa tggacggttg
     3541 atgtccatga ttgctaatat gcttgcactg ataattaccg taaacaggac cgataaaata
     3601 agaggacgaa gccctgcgtc ggaccctaaa ctgatgctag agaaccattt aatgcgtttc
     3661 atctaatttg cctatattct atgataggaa agccctcctt taaattacag tttttatata
     3721 aaaaaagaaa ccctctaaag ggtttccaaa gagtgagcca ccggtgggat acgaacccac
     3781 gacctgacga
//
LOCUS       DAOREF010000181         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14819_length_3789_cov_4.567220, whole genome shotgun sequence.
ACCESSION   DAOREF010000181 DAOREF010000000
VERSION     DAOREF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14819_length_3789_cov_4.567220"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2719
                     /locus_tag="PKI33_05030"
     CDS             <1..2719
                     /locus_tag="PKI33_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36400.1"
                     /translation="RNLAVMLLLIAMFVNTFVLIFIINGLSLIALTSVTVLTFTIVRR
                     ALPARYATSGIIVAIIFSGLLLVIDNLLGPNRISVPGLDAYMPYIVGIILIPLVYGFT
                     RNFSNFSLQTKITLGILLTGGVTVSVIVVFGLNRFNFISSFLADKYEDNAITKTEEEI
                     SSTTALEANKIDTLFFEIQKDLSALAEYRVNLEQQKGLFQQGSYWNAAEKLIQLPDGQ
                     YGNSSLEFASVYIPNTYTITNSIIEDLNTSIYLDMTALGFLKSHSEVVAVYYVSALGY
                     TVYYPNISLAQNVPPDFDPIQQSFYTIATPSNNPDKLPRLTLPYKDPAGTGLIVTLSV
                     PVYSGNIFKGVISADIQLAKVTEAVSKIQISSTGIPFLVNEDGLILAMNDAGYNYFGL
                     NPENVPVNENPKQTIFDTPLPELQKVVQEIPMSEAGLSKFKINNVDTYVSISNLETTG
                     YKLVFIAPVSELSSEVIALREAITSQINQIIQNITIILIILFVGTFIASLLVGQVITS
                     PLKRLTETVEEIASGNLSSRATIETGDESGTLARAFNLMADQLTETLQGLEDRISVRT
                     KELESLSQTNVYRATRFEAIARISRIISSTRDLEQLLTQITETISEQLGYYHVGIFLI
                     DVHKENAVLVSANSEGGKVMLARGHRLGVGEQGIVGYVTKFGLPRIAVDVGQDATYFN
                     NPDLPETHSEVALPLRSGSEIIGALDVQSKLTNAFSDEDVNILLTLADQVSIAIQNAR
                     SFQQSREALERAERAAAQLTEQQWSQIRKGQKVAGFHFDGIQTQELKSQQKGNQNNLA
                     IPIILRGVQIGSIKLSTPDADRKWDNNEIAMAQATAERTALAIETARLLEEAQKRASK
                     ERTIGHISSTIGSLVNIENIVQTVIQELGDTLPGTDVAIQFTNRQSEQQ"
     gene            2737..>3789
                     /locus_tag="PKI33_05035"
     CDS             2737..>3789
                     /locus_tag="PKI33_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36401.1"
                     /translation="MLEKLLNLIRSEEDQDPSFIRLTRNILIFVMVVTFAVIPLVTGI
                     AGSPQNRLAFTALSILLVLEAASFYFITRGNVLPAKVVVPLGLVIAITTIANNTNGVR
                     DTSFFALPMILIVSATLLGKRSLTLIVPVAVVATAIIAITDISRGRRAGLVGVDDAII
                     IPLLMAASGVITHLLISRLNESTVRAQKSEQLQKIENQELLVLRATLEERVRERTAEL
                     EIANKVNEKRALQFSAIAKVMNAISSIQTLDELLPLVAKVISEEFNFYHVGIFLLDSR
                     KELAVLRASNSEGGQRMLARNHSLSVGQTGIVGYVTATGQPRIALDVGADSAYFDNPD
                     LPNTRSEIALPLRYAKQ"
BASE COUNT         1134 a          827 c          770 g         1058 t
ORIGIN      
        1 aagaaattta gcagtcatgc ttcttcttat agcgatgttt gtaaatactt ttgttctaat
       61 ctttatcata aacggattga gcctcattgc gctgacatct gttactgtac ttacattcac
      121 aattgtaagg cgagccctgc cggctcgtta cgcaacatct ggcattattg tggcaatcat
      181 tttttctgga ttgctactgg tcatagataa tcttttaggt ccgaaccgca tcagtgtgcc
      241 aggattggat gcctacatgc cctatattgt aggcattata ttaattccgt tagtatatgg
      301 gtttacaaga aacttcagca acttcagcct tcaaacaaag atcacactag gtattcttct
      361 tactggtggc gtcaccgtta gtgttattgt tgtattcggt ctaaatcgct tcaattttat
      421 tagtagtttt cttgcagata aatacgaaga caatgctatt actaaaacag aagaagagat
      481 atcaagtaca acagctttag aagcgaacaa aatcgacact ctcttttttg agattcaaaa
      541 ggatcttagc gctcttgctg aataccgagt aaatcttgaa caacaaaaag gtctttttca
      601 gcaaggaagc tattggaatg cggcagaaaa attgattcaa ctgccggatg gtcaatatgg
      661 gaattcaagt ttagagtttg cttcggtcta tatccctaat acatatacga ttacaaatag
      721 catcatcgaa gacctaaata cttcaattta tcttgacatg actgccttag ggtttctcaa
      781 gtcacactca gaagttgttg ctgtttatta cgtcagtgct cttggctata ctgtttacta
      841 tccaaatatc agtttggcac aaaatgtgcc accagatttc gaccctattc aacaaagttt
      901 ttataccatt gcaacaccct caaataaccc ggacaaatta ccgcgcttaa cccttcccta
      961 taaagatcct gctggcacag gtttgatcgt tacattatct gtccctgtgt atagcggaaa
     1021 tatcttcaaa ggggttatta gcgctgatat tcaacttgca aaagtaactg aagctgtttc
     1081 taaaattcag atcagttcta caggtattcc atttctcgtg aatgaagatg gtctcattct
     1141 tgctatgaat gatgccggat ataactattt tgggctaaac cccgaaaatg ttccagtcaa
     1201 cgaaaatcca aaacagacta tttttgacac cccgctacct gaacttcaaa aagtcgttca
     1261 agaaatacct atgtctgaag caggtctttc taaattcaaa atcaataatg tagacacata
     1321 tgtgtccatt tcaaatcttg aaacaacagg gtacaaactg gtattcattg caccagtaag
     1381 cgaattaagc agtgaggtaa tcgctctacg cgaggcgatc acctcacaaa tcaaccaaat
     1441 tattcaaaac attacgatca ttcttatcat cttatttgtt ggtactttta tagccagcct
     1501 tttggttgga caggttatta caagcccgct aaagcgcctg acagaaaccg tggaagagat
     1561 tgcatctgga aatctatctt cccgtgcaac aatagaaaca ggtgatgaaa gcggaaccct
     1621 cgcacgcgcc ttcaacctaa tggcagatca actgacagaa actctacaag ggctggaaga
     1681 tcgcatttct gttcgtacta aagaacttga aagcctcagc caaacgaatg tttaccgtgc
     1741 aacgcgcttt gaagcaatcg ctcgtatttc tcgtatcatt agttctactc gtgatctcga
     1801 acagcttcta actcagatca cagaaacaat ctctgagcag cttggctatt atcatgttgg
     1861 tatcttctta attgatgttc ataaagagaa tgcagtcctt gtgtcagcaa atagtgaagg
     1921 tggtaaggtc atgttagcca gaggtcaccg tctaggagtt ggtgaacaag gtattgtagg
     1981 gtatgtcact aaatttggct taccacgtat tgctgttgat gttgggcaag atgcgaccta
     2041 cttcaataac ccagatcttc cagagaccca ttctgaagtt gccctccccc tccgaagcgg
     2101 ttccgaaatt ataggtgcat tggatgtaca aagtaaatta acaaatgcgt ttagtgatga
     2161 agacgttaat atccttttga cacttgcaga ccaggtgagt attgcgattc aaaatgcaag
     2221 gtcattccaa caaagtcgtg aagcgcttga aagagcagag cgagcggcag cgcagttaac
     2281 tgaacaacaa tggagtcaga tccgtaaagg gcaaaaagtc gccgggttcc actttgatgg
     2341 cattcagact caggaactta agtcacaaca aaaaggtaat caaaacaacc ttgccattcc
     2401 gatcatattg cgcggcgtgc aaattggctc tattaagcta agcacacctg atgctgaccg
     2461 aaaatgggac aataatgaaa ttgccatggc acaggcgacc gcagaaagaa cagctcttgc
     2521 tattgaaacc gcacgccttc tggaagaagc acaaaaacgt gcttcaaaag aacgcacgat
     2581 tgggcacatc tcatccacga tcggtagttt ggtcaatatc gaaaacattg tacaaactgt
     2641 cattcaggaa ttaggtgata cattgcccgg cactgatgtt gccattcaat ttacaaacag
     2701 acaatccgag cagcaatagc cgcccaggag ccaaatatgc tagaaaaatt actaaaccta
     2761 attcgaagcg aagaagatca agacccgtca tttattcgtt tgacgcggaa tattttgatc
     2821 tttgttatgg tcgtaacttt tgcagtgata ccactagtga cggggattgc tggttcacct
     2881 caaaaccgcc ttgcatttac cgccctgtca atattattgg tattggaagc tgcttcgttc
     2941 tacttcatta ctagagggaa tgtattacct gccaaagtag tagttccact tgggttggtc
     3001 atcgccatca ccaccatcgc aaacaacaca aacggtgtac gtgatacgtc tttcttcgct
     3061 cttcctatga tcttgatcgt ctctgctact ttattaggca aacgttcgct gactcttatt
     3121 gtgccagtag cagtagttgc gacagcaata attgccatta cggatataag ccgtggtcgt
     3181 cgagcaggct tggtaggtgt tgacgacgca atcatcattc ctttgctaat ggcagccagc
     3241 ggcgtaatca cacatctgtt gatatctcgc cttaatgaaa gtacggtacg ggctcaaaaa
     3301 agtgaacagc tacaaaaaat agaaaatcaa gagttacttg tcttgcgcgc cacccttgaa
     3361 gaaagggtac gagaaagaac ggcagaactt gaaatagcaa acaaggtcaa tgaaaaaagg
     3421 gcgctacagt ttagtgccat tgcaaaagtt atgaacgcaa tttcatccat tcaaactctt
     3481 gatgaattgc tgcctcttgt tgcaaaagtg atcagcgaag aattcaattt ttaccatgtt
     3541 ggtatctttc tgcttgatag caggaaagag ctagccgttc tacgcgcttc aaacagtgaa
     3601 ggtggacaaa ggatgcttgc ccgtaaccac tctctttctg ttggacaaac aggtattgtg
     3661 gggtacgtaa cagcaacagg acaaccccgc atcgctctgg acgtgggagc ggattctgct
     3721 tactttgaca atccagacct cccaaacacc cgctctgaga tcgctttgcc tctgcgatac
     3781 gccaagcaa
//
LOCUS       DAOREF010000182         3767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_14963_length_3767_cov_4.244881, whole genome shotgun sequence.
ACCESSION   DAOREF010000182 DAOREF010000000
VERSION     DAOREF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14963_length_3767_cov_4.244881"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..165)
                     /locus_tag="PKI33_05040"
     CDS             complement(16..165)
                     /locus_tag="PKI33_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36402.1"
                     /translation="MYLISETFTPAMLVVRLVEPGWSVGQYTVFAQDLIIQDQYLLQL
                     KLKLR"
     gene            155..1138
                     /locus_tag="PKI33_05045"
     CDS             155..1138
                     /locus_tag="PKI33_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNM36403.1"
                     /translation="MRYKPFGEVRYTWRASQTTTPAYALSRFTFTGQYSHMDDPSTSG
                     VTEGFGLMFYNARWYDPALGRFAQADTIIPAGVQGLDRYAYANNNPIHYTDPSGHVVC
                     EVGEPCGRGATYVEETSTAQMNKNLEDKLGNEYGVTLQKGTRSWDSRNIYAAYAGMFN
                     VDKALNGQTESILGPTTFILNWHSGEGDYRGTSYPGLTIFEATTTIPSQNLYHEFAHL
                     LNYASGGAFERRVDAQTHEVAGNYIFGGSAGIMSEARGFGLKFSDLTDPNYGIITDVI
                     QHPDTDPSEQWSDLFANYAAGNIDMSSSAGQEIHTFVNSSLTPDISLISGR"
     gene            1165..2043
                     /locus_tag="PKI33_05050"
     CDS             1165..2043
                     /locus_tag="PKI33_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36404.1"
                     /translation="MRHLTYRSLIFLMVYLYGCSSLNQEKPTIPPVNRATLFAATIEN
                     PITPTITLTASPIPTLSPQVAENAIKQMLNEETQCARPCLFGIIPEQTTLKEIQNFYE
                     HKNMNFKRTYQEGRKDYYEAGFGFDESWGIILTNIVEAGIVRNLTLGLSFVNYTGSTP
                     QQEWLAFRPNAVLERYGRPSNIELNLSFPVESGFPPGTAWYDIVMYFPDSDLIVKYAY
                     ELTNASPKITACPRTDKSLGMSMWLGKEPHSPPVGIVSLDKATTLSLEEFYDFLTTNS
                     QTVCFDLNRKDFFSDP"
     gene            2452..2904
                     /locus_tag="PKI33_05055"
     CDS             2452..2904
                     /locus_tag="PKI33_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36405.1"
                     /translation="MKNKTTKTISVATLISILAGILVFAYLAVRNKDEFRRDIQVKNV
                     SICYKVNADNYYKSDNCKDIFTEHEVNDLYVCGYIDAPYSEIKNLSLVITSNQSTEPF
                     FANPPNDKFGVGYFCRKITLPPNGRVDAYQIDFYYFREIVASIRFDIH"
     gene            complement(2923..3240)
                     /locus_tag="PKI33_05060"
     CDS             complement(2923..3240)
                     /locus_tag="PKI33_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36406.1"
                     /translation="MYLISETFTPAVSVVMEVKPKWSPRKYSSLSYAKAHDQTVADDY
                     FAAMQRVEQRLEIVPAQEKEKTNEDVKLRLVQIIKQLELSELGIEERILLAIHLNDNE
                     DIR"
     gene            3477..>3767
                     /locus_tag="PKI33_05065"
     CDS             3477..>3767
                     /locus_tag="PKI33_05065"
                     /EC_number="3.5.4.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="catalyzes the transformation of hydroxyatrazine to
                     N-isopropylammelide and ethylamine in the atrazine
                     degradation pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNM36407.1"
                     /translation="MTTLLVKHATIVTMDDHQREIADGGLFIRDGFIEQVGPTSSLPA
                     TADEVLDLTGHVVLPGLVNTHHHFYQTLTRAVPAAQDANLFNWLKTLYPIWAR"
BASE COUNT         1084 a          907 c          801 g          975 t
ORIGIN      
        1 catcgatact gttttttatc ttaactttag tttcaattgc aatagatatt gatcttggat
       61 aattaaatcc tgtgcaaaga cggtgtattg acctacagac caccccggct ctaccagcct
      121 gaccaccaac atggcaggcg taaaggtctc ggagatgaga tacaaaccgt ttggcgaagt
      181 gcgctatacg tggagggcca gtcagaccac cacaccggcg tatgccctat cccgctttac
      241 gtttacggga caatattccc atatggatga tcccagcaca agcggagtca ccgagggctt
      301 tgggttgatg ttctacaatg cccgctggta tgaccctgcg ttggggcggt ttgcccaggc
      361 ggacacgatc atccctgcgg gagtgcaggg actggataga tatgcgtatg caaataacaa
      421 ccccattcat tatacagacc cctccgggca tgtggtttgt gaagttggag agccgtgcgg
      481 gagaggcgca acatatgtcg aggaaacttc cacagcccag atgaataaaa acctagaaga
      541 taagttgggg aacgaatatg gtgttacatt gcaaaaagga actcgttcat gggatagtcg
      601 taatatttat gcagcctacg caggtatgtt taatgttgat aaggcactca atggtcaaac
      661 cgagtctata ttaggtccaa ccacatttat attgaattgg cattctggcg aaggagatta
      721 tcgcggaact tcctatccag gtctcactat atttgaggca accactacaa ttccatccca
      781 aaatctttac catgaatttg ctcacttatt aaactacgca tcaggtggtg catttgagcg
      841 gagagtggat gcacaaaccc atgaagttgc tggaaactat atctttggag gtagtgcagg
      901 gataatgagc gaagcacgtg gttttggcct gaaattcagc gatttgaccg acccgaacta
      961 tgggattatt acagacgtga tacaacaccc ggacaccgac ccttctgaac aatggtctga
     1021 tctgtttgcg aattatgctg ctggaaatat cgatatgtcg agtagcgcag ggcaggagat
     1081 acacacattt gtcaatagtt ctctgacccc cgatatttct ctaatatctg gcaggtaaca
     1141 attttattgg agtaaatttt cgccatgcga cacttaacat atcgttcgct tatttttttg
     1201 atggtttacc tatatggatg ttcatccctg aatcaggaaa aaccaacgat ccctcccgtg
     1261 aaccgcgcaa ccctgtttgc agccacgata gaaaacccta ttacaccaac aattacgcta
     1321 actgcctctc caattcctac attaagtcct caagtagctg agaatgccat aaaacaaatg
     1381 ctgaacgagg aaacacaatg cgccaggcct tgcttgttcg gtatcattcc agagcaaacg
     1441 acgctgaaag aaatacagaa tttttatgaa cacaaaaata tgaatttcaa acgcacatac
     1501 caagaaggga gaaaggacta ttatgaagca ggatttgggt ttgatgagag ttggggcatt
     1561 atcttaacaa atattgtcga agctggcata gtacggaact taacgcttgg tcttagcttt
     1621 gttaactaca caggctctac ccctcaacaa gagtggttgg cattcagacc taatgccgtt
     1681 ttggaaagat atggacggcc gtccaatatt gaattaaact taagttttcc cgttgagtcg
     1741 gggtttcctc ctggaacagc ttggtacgac atagtcatgt attttcctga ctctgatttg
     1801 atagtcaagt atgcgtatga actaacaaat gcgagtccaa aaataactgc ctgcccacgc
     1861 accgataaat ctttgggcat gagcatgtgg ctgggcaaag aacctcatag tcctcctgtg
     1921 ggaattgttt cattagacaa agccacaaca ctttctttag aagagtttta tgattttctc
     1981 acgacaaact cccaaacagt ttgttttgat ctgaatagaa aagatttttt ctccgatcct
     2041 taaaattcaa aatctaaaca aaatttatca actcgctttt gaaaaacaaa aggacgaacc
     2101 aaacgtgagg ctcagtccgt cttttgactt ggggagcgct ttcattgcac aacgctgaaa
     2161 gcgacagcag attaaaaaca aaatatcctc agaccctgtc ccaccgggat gttgcgcgtg
     2221 ggcgaagtgc gctttgcgtg gaaggcgagt cagaccacca caccggcgta tgccctgacc
     2281 cgcttcagac ccaatcagtg aaaggcccat cgaatatcat cagtcaagta ttacataagg
     2341 aggaaatata gctggcaacg gttttgatat tgggttgttg ggcgggggag catatacagt
     2401 gaatataacc tcatatcaaa taccattttg gccataaagg aaatgggaac aatgaaaaat
     2461 aagacaacaa aaactatttc agtagcaact ttgatttcaa tattggctgg cattcttgtg
     2521 tttgcatacc tagcggtacg taataaagac gaattccgcc gagatataca agtaaaaaat
     2581 gtttcaattt gctacaaggt aaatgcggat aattattata aatccgataa ttgtaaagac
     2641 atatttacag agcacgaagt taatgacctg tatgtatgcg ggtatataga tgccccatat
     2701 tcggaaataa aaaacctatc acttgttatt acgagcaacc aatccactga accgtttttc
     2761 gctaatccgc caaatgacaa attcggcgtt ggatattttt gtagaaaaat caccttgccg
     2821 cctaatggtc gagttgacgc ttatcaaata gatttttact acttcaggga aatagttgcc
     2881 tcaattagat ttgatattca ttagaatggt caacacaaat actcacctta tatcctcgtt
     2941 gtcgttcaag tgaattgcca ataagatacg ttcctcaatt cctaattcgg aaagttccag
     3001 ttgtttaata atctgcacca accgcaactt gacatcctca ttagtttttt ccttctcctg
     3061 agctggcaca atctccaacc tctgctccac cctctgcatc gcggcgaaat aatcatccgc
     3121 caccgtctgg tcgtgcgcct tggcataact gagcgaggaa tacttcctgg gagaccattt
     3181 gggtttcacc tccatcacca ccgatacagc aggcgtaaag gtctcagaga tgagatacaa
     3241 accgtttggc gaagtgcgtt atacgtggaa ggcgagtgaa accaccgcct tccactttcc
     3301 aactttgtaa cctaccagta cgcatcctcc taattgacat ccacccactt ctttgctaaa
     3361 ctataagttg tcagacaaat tagtctagtt gtttcggcgc cctgccaaag acccactccg
     3421 cacaggcgct tcccctgcca tcaagcaggc gacccttgga ccaagagact acccccatga
     3481 caactctcct cgtcaagcac gcaaccatcg taaccatgga cgaccaccaa cgcgagatcg
     3541 ccgacggtgg tctatttatc cgcgacggct tcatcgaaca ggtcgggccg acctccagcc
     3601 tgcctgccac tgccgacgaa gtgctcgacc tcaccggcca tgtggtgctg cccgggctgg
     3661 tcaacacaca ccaccacttc taccagaccc tcacgcgcgc cgtacccgcc gcacaggacg
     3721 ccaacctctt caactggttg aagaccctct atcccatctg ggcgcgc
//
LOCUS       DAOREF010000183         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15041_length_3754_cov_4.931062, whole genome shotgun sequence.
ACCESSION   DAOREF010000183 DAOREF010000000
VERSION     DAOREF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15041_length_3754_cov_4.931062"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2305)
                     /locus_tag="PKI33_05070"
     CDS             complement(<1..2305)
                     /locus_tag="PKI33_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNM36408.1"
                     /translation="MMTQSGNPSGKRKTIAVLGAQLSRVWGSEFMAGVLDAAHAVDFN
                     VVSFVGGKPSALQSHDGHSYGLYDLINPEQIDGVLLSADIAHGLSRDALQKFVEFLAP
                     LPVASFAVPSKGVPAVIADNDSGMRAIVRHLIEDHNYQRIAFVRGPVGQVEADQRYHA
                     FLDELKAHNIRFDQHLELEGDYSTESGRAAVRTLLDERGMAVQAIVASNDRMAFGVLE
                     ALQQRGITVPDKVAVTGFDDVSESQSMGVPLTTVRQSFYDAGAKTFELLLKRINGEEV
                     DDINLLPSSIVVRWSCGCLPESIHRAVVLPREVAHTGRLENKRVKAINALLAAAGVPE
                     TTPDRDVYVENFGVAWDTFLYSLRETDKSDAFLRSIQGMVEILQKHGYDFYTWQNVIS
                     TFRKYALGGISSTTTTLRAENLFQQARMLAGELSQRAQAYNRLQYVKQEELLNNFSFS
                     MASAVSFDDIGDAIARHFPILGIERWYVMYYGETDDAPGSISSPPPQSYRLLMQYDDK
                     RFTMPDDRSRLATGRLVPRGKTPEKRRYDAVVMPLSLATNRFGFMWAEMGPDDWDIYV
                     RVRNLLSSALLRTMLVNQREQAKREVDQLYAAEQARRHGAETLARSMRQLSSLKNVEQ
                     LPEQILEQLSQALPYDRGVLFMEDVNGEPQIQAHRGMPTNADMASFRLGMNGNDFYTT
                     VSRKGETLVVSDINLVENWSQPEWLARDRSWMGVPLYLKDNVIGLLMVSRADRSFTHD
                     DGLLATTFASQATVALENARLYHEVTGI"
     gene            complement(2431..>3754)
                     /locus_tag="PKI33_05075"
     CDS             complement(2431..>3754)
                     /locus_tag="PKI33_05075"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNM36409.1"
                     /translation="MFLFNADNTPLGGDYAYAVLAHEFQHMIHWNNDLNETSWLNEGS
                     SELAVLLNGYETGGFDTLYISDPDLQLNDWPNDQGATSPHYGAGFLFMTYFLDRFGEE
                     ATQALVKDPANGLDSVDDVLQQIGATDKLTGQPITADDFFMDWALTNLLLDPSIADGR
                     YVYNNYPAAHRASATETIYNCPQEPLLRDVHQYGVDYIAIECAGDFTINFTGSTVTGL
                     LPADPYSGDYAFWSNKGDESNMTLTHEFDLTNASGPVDLSFRTWFDIEEDWDYVYVEV
                     SEDGETWQILTTPSGRGTDPSGNSYGWGYTGVSGDWIEEKVDLSDYAGKKVFVRFEYI
                     TDAAVNGEGFMVDDVKVDAAGYSSDFEADDGGWVAEGFVRVQNVLPQTFKLALIKSGD
                     DNVTMIPVNADQTAEIPVSLKSGETAYLVVTGTTRFTRELATYQIEIR"
BASE COUNT         1024 a          988 c          858 g          884 t
ORIGIN      
        1 taatgccagt cacttcgtga tacaaacggg cgttttccaa agcaacggtt gcttgtgaag
       61 caaatgtcgt cgcaagcaaa ccgtcatcgt gagtaaatga ccggtccgct cggctgacca
      121 tcaagagccc aatgacatta tcttttagat ataaaggcac acccatccaa gaacggtcac
      181 gggcaagcca ttcaggctga gaccaatttt caaccaagtt gatatcgctc accacaagag
      241 tctcgccttt gcgtgaaacg gtcgtgtaaa aatcattacc attcattccc aagcggaaag
      301 atgccatatc cgcattggtt ggcataccgc gatgggcttg aatctgcggc tcaccattta
      361 catcttccat aaacagaacg ccgcgatcat acggcaatgc ttgactcaat tgctccagaa
      421 tctgttccgg taactgttca acatttttca atgaggataa ttgacgcatc gaacgtgcca
      481 acgtctcggc accatgacgc cgtgcctgtt ccgccgcata aagttgatcc acttcacgtt
      541 tggcttgctc acgttgatta accaacatcg tacgcaagag cgcgcttgaa agcaagtttc
      601 gcacgcggac gtaaatatcc caatcatcgg gtcccatttc tgcccacata aacccgaagc
      661 gatttgttgc caaagagagc ggcatgacta cggcatcgta acgacgtttt tcaggagtct
      721 ttccgcgtgg cacaaggcga cctgttgcaa gtcgagaccg atcatccggc atggtgaagc
      781 gtttatcgtc atattgcatg agcaaacgat aactttgcgg cggcggtgaa gaaatcgaac
      841 ccggcgcatc gtccgtctcg ccataataca tcacatacca gcgctcaata ccaagaatgg
      901 ggaaatgtct agcaatcgca tcgccgatat catcgaacga aacagccgat gccatggaaa
      961 aactaaagtt attaagcagt tcttcctgct ttacatattg aagccggtta tatgcctgag
     1021 cccgctgcga caactcgccc gccaacatac gtgcctgctg gaaaagattt tcagcacgca
     1081 atgtagtggt tgtgcttgaa atgccaccta acgcatattt acggaaagtt gagatcacgt
     1141 tttgccaggt atagaagtca taaccatgct tctgaagtat ttcaaccatg ccttgaattg
     1201 accgtaagaa cgcatcactc ttatcggttt cgcgcaaact gtaaaggaag gtgtcccatg
     1261 ccacaccaaa attttcaaca tagacatcgc gatcaggtgt agtttccggc acgcctgctg
     1321 ctgcaagcaa agcattgatg gctttcacac gtttgttttc aagccggcct gtgtgcgcta
     1381 cttcacgtgg caacacaact gcacgatgaa tactctctgg taaacaacca caagaccaac
     1441 gcactacaat gctggatggc aacaaattaa tatcatccac ttcttcgcca ttgatgcgtt
     1501 tcaggagaag ttcaaatgtc tttgcacccg catcataaaa cgattggcgg acggtggtta
     1561 aaggcacccc catagattgt gactcactga catcatcgaa acccgtaacc gcaaccttat
     1621 caggcactgt aataccgcgc tgttgtaacg cttccaacac gccgaatgcc attcggtcat
     1681 tcgaagcgac aatggcttgc actgccatgc cacgttcatc aagcaaggtg cgcactgcgg
     1741 cacgtccact ttcggtggag taatcgcctt ctaattccaa atgttggtcg aaacgaatgt
     1801 tatgagcttt caactcatcc agaaaggcat gataacgttg atctgcctct acctgcccca
     1861 cgggtccacg gacaaaggcg atgcgttgat aattatggtc ttcgatcaag tgccgtacga
     1921 tcgcacgcat accgctgtca ttatccgcga tcacagcagg aacgcccttg cttggcacag
     1981 cgaatgaagc aaccggcaaa ggcgcgagaa attcaacaaa cttttgaagt gcatctcgtg
     2041 aaagcccgtg ggcaatatct gcagagagca aaacaccgtc tatctgttcg ggattaatga
     2101 ggtcgtataa accgtaagaa tgtccatcat gagattgaag cgccgaaggt ttcccaccta
     2161 caaaagaaac gacattgaaa tccactgcat gagcggcgtc caacacgccc gccataaact
     2221 cactgcccca cactcggctg agttgtgcac cgaggacggc gatcgtcttt cgcttgccag
     2281 aaggatttcc gctttgggtc atcatggagt tacaaaatta tacggcagga tgcctgagcc
     2341 aaaaattgca attttgcaag acaatctccg ccttaaaaaa attgacagcc gccataaaag
     2401 cggctgtcaa tgtagaaatt tcagaaaagc ttagcggatc tcgatctgat aggtcgccag
     2461 ctcacgtgtg aagcgggtcg ttccggtcac aaccaagtaa gccgtttcac cagatttcaa
     2521 cgaaaccggg atctctgctg tctggtctgc gttgaccggg atcatggtca cattgtcgtc
     2581 gccagatttg atgagcgcca gcttgaaggt ttgtgggagg acattctgga cgcgcacgaa
     2641 tccctccgcc acccaaccgc catcatccgc ctcaaaatca gacgagtagc ctgctgcatc
     2701 gaccttgaca tcatctacca tgaagccttc tccattgact gcggcatcag taatgtactc
     2761 aaagcggaca aagactttct taccagcgta atcagaaagg tcaaccttct cttcgatcca
     2821 atcgccagaa acaccggtat acccccagcc atacgaattg ccgcttgggt ctgtacctct
     2881 accagaaggt gtagtgagaa tttgccaagt ctcgccgtct tcagagactt ccacatacac
     2941 atagtcccaa tcctcttcga tatcaaacca agtgcggaag gacaaatcaa ccggtccgct
     3001 ggcattggtg agatcgaatt catgcgtcaa tgtcatgttg gactcatcgc ccttgttcga
     3061 ccaaaaggcg tagtcaccag aataggggtc agctggcaaa agcccggtca cggtagagcc
     3121 ggtgaagttg atggtaaagt cgccagcaca ttcgatggcg atatagtcca caccgtattg
     3181 gtgaacgtcc cgcaggagcg gctcttgcgg gcagttataa atcgtttcag ttgcagaagc
     3241 acgatgtgcc gccggataat tgttgtacac atagcgtcca tcagcaatgg aggggtcgag
     3301 caaaagattc gtcagtgccc aatccatgaa gaaatcatct gccgtaatcg gctgaccggt
     3361 caatttatcg gtcgcaccga tttgttgtaa gacatcgtcc acactatcca acccattggc
     3421 tggatctttc acgagtgctt gggtggcttc ttcaccgaaa cggtcgagga agtatgtcat
     3481 gaaaaggaag cccgccccat aatgcggcga cgtagcaccc tgatcgttgg gccagtcatt
     3541 taattgcaag tctgggtctg agatataaag cgtatcaaaa ccaccagttt catacccatt
     3601 aagcaaaact gccaattcag aagaaccttc gttcaaccag gaggtttcgt tcaagtcatt
     3661 gttccaatgg atcatgtgct ggaattcatg tgccaaaact gcataggcat aatcgccgcc
     3721 gagcggagta ttatctgcat tgaacaggaa catc
//
LOCUS       DAOREF010000184         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15245_length_3725_cov_3.577657, whole genome shotgun sequence.
ACCESSION   DAOREF010000184 DAOREF010000000
VERSION     DAOREF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15245_length_3725_cov_3.577657"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..966)
                     /locus_tag="PKI33_05080"
     CDS             complement(67..966)
                     /locus_tag="PKI33_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNM36410.1"
                     /translation="MKIIICDKTEKEYIEQMRAAGLTVDVRDDITPEQLMVELPNYDG
                     MVVRSRTKVRKDLIDVCPNLKIILRGGAGLDTIDHEYAKEKGITVMNTPLANSKSVAE
                     LTIGYMLMMARSLYAASATMKAEKWDKKSFNGDEIGGKTLGLIGIGNIGKEVTKRALA
                     MEMTVVAYDPYVKEMAGVKLVSLDELLAQSDYISLHLPKTKESSNMIGKEQFAKMKNG
                     VRIVNCARGGIVNEEALYEALTSGKVAGAALDVFNEEPPVDWKLAKLDNVIASPHIGA
                     ATKEAQARVGAEVAEKLIAFAKK"
     assembly_gap    1514..1613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1614..2617
                     /gene="serC"
                     /locus_tag="PKI33_05085"
     CDS             <1614..2617
                     /gene="serC"
                     /locus_tag="PKI33_05085"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNM36411.1"
                     /translation="NFKGTGMSVMEISHRSKEFEAVIQTAEADLRELLGIPSNYKVMF
                     LQGGATLQFAMVPMNLRAAGASADYVVTGSWSKTAVKEAKKLGATRVAATTEATNFNS
                     LPEKYDFDPKAAYAHITMNETIHGVEFFNEPVVPDGVPLVCDTSSNFISRPIDVSKYA
                     FLYAGAQKNAGPSGVTVVIARDDMIARTPENLPVMLDYKTLAESGSLHNTPPSFAIYM
                     VGLVFKWAKKVGGLAAIEKINREKAGIVYNAIDQSGGFYRGHTVPEARSLMNIPFRLP
                     SEELEETFAKEAKKNDLIGLKGHRSVGGMRASVYNAMSIEGAKELAKFMAEFQKKNG"
     gene            complement(2777..3331)
                     /locus_tag="PKI33_05090"
     CDS             complement(2777..3331)
                     /locus_tag="PKI33_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36412.1"
                     /translation="MKKIFITLCLSVMLLACGTPTVPPEVVSEAYPITEPALVLPDYK
                     NATYLIEGSFVTLADGIFEVEIAPGSTSKITTRYFGNEAIGDLNKDGKSDVAFLLTQD
                     TGGSGTFFYIVVALQTASGTEGSNAIFLGDRIAPQNTIIENGVITVNYADRNPGEPFT
                     TPPSLGVSKYFKIENNILTEVQNP"
     gene            complement(3343..>3725)
                     /locus_tag="PKI33_05095"
     CDS             complement(3343..>3725)
                     /locus_tag="PKI33_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNM36413.1"
                     /translation="LWMIGFEDKPEYSAEICIFELKGYNIKKKSALIGYGVHPFGDPS
                     IKDEFYEEEFSLDVTQFNTYAAEWTEREVHFYINDQKIRTIQQSPQYPMQFMLNIYDL
                     SKSPKHDRPMQFIVDYVSGYTLSK"
BASE COUNT          850 a          873 c          950 g          952 t
ORIGIN      
        1 cattaaattg taggggcgag gtctcctcgc ccagggcggg gggaccccgc ccctacggga
       61 tatgaattat ttcttcgcga acgcgatcaa cttctctgcc acttccgcgc cgacgcgagc
      121 ttgggcttcc ttcgtcgctg cgccaatatg cggggatgcg atgacgttat cgagcttggc
      181 gagtttccag tcaacggggg gctcttcgtt gaaaacgtcc aacgcggctc ctgcgacctt
      241 tccactggtc agcgcttcgt agagtgcttc ttcgttgacg attccaccgc gcgcgcagtt
      301 gacgatgcgc acaccattct tcatcttggc aaactgctcc ttgccgatca tattgctgga
      361 ttctttggtc ttcggcaaat gcaagctgat gtaatctgac tgggcgagca attcatctaa
      421 tgaaaccaac ttcacgccag ccatttcttt cacatacggg tcgtaggcaa ccacggtcat
      481 ttccatggcg agggcgcgtt tggtcacttc cttgccgatg ttgccgatgc cgatcaagcc
      541 aagagtcttg ccgccgatct cgtcgccgtt gaaacttttc ttgtcccatt tttcggcttt
      601 catcgtggca gaagccgcat aaagtgagcg tgccatcatc agcatatagc cgatagtgag
      661 ttccgccaca gacttggagt tggcgagggg agtgttcata accgtaatgc ctttttcctt
      721 ggcatactca tggtcaatgg tatcgagccc ggcaccgccg cgcagaataa tcttgaggtt
      781 ggggcacaca tcgatcaggt ctttgcggac cttggtgcgc gaacgcacga ccatgccgtc
      841 gtagttgggc agctccacca tcaactgttc tggggtgata tcgtcgcgca cgtccacggt
      901 cagcccggcg gcgcgcatct gttcgatgta ttctttttca gttttgtcgc agataatgat
      961 tttcattgga ttctcctata ttttagaaag caaacttttg gtttcgtagg ggcgaccctt
     1021 ccggtggggg agattgtctg ttaaacccag gcgggtcgcc actacgtgta cggtaaatta
     1081 ataacaggaa ccgaaccgcc gaagcggcta tgttcctgtt tgtgaattca tgcgcaacac
     1141 ggtaatcatt ctaacaatgc gatatacctt tgtcaacgat ggaaatcagc ccatatgggt
     1201 gcttattgtt cttgtagaat tgtctgacaa actatataat gatttagata cgattgtcta
     1261 attttgcact aaaatgttcc ggtgtagaca actgcctatt gatctgtatc aagcccaaat
     1321 cttcggatga ggcgtttgtt gtccaatatc ataaacagga gtatgaatcc atgtctgatt
     1381 taaaacgtgt atataatttc aaccccggtc cgggtgtgtt gccgcttgaa gtgttgcaag
     1441 aagcgcaagc tgaattgctg aacttcaaag gcacgggcat gtcggtgatg gagatcagcc
     1501 atcgttcgaa ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgaactt
     1621 caaaggcacg ggcatgtcgg tgatggagat cagccatcgt tcgaaggaat tcgaagcggt
     1681 gatccaaacc gccgaagccg acctgcgcga gttgttgggc atcccttcta attacaaggt
     1741 gatgttcctg caaggcggcg cgacattgca atttgcgatg gtgccgatga acctgcgtgc
     1801 cgccggtgct tctgccgact acgtggtcac cggttcatgg agcaagaccg ctgtgaagga
     1861 agctaagaaa cttggcgcga cccgtgttgc cgcaacgacc gaagcaacga atttcaatag
     1921 cctgcccgag aaatatgact ttgacccgaa ggcggcttat gctcatatca ccatgaatga
     1981 aaccattcac ggtgtcgagt tcttcaacga gccggtggtt cccgatggtg tgccattggt
     2041 gtgtgatacc tcttcgaact ttatcagccg cccgatcgat gtttcgaaat atgccttcct
     2101 gtatgcaggt gcgcagaaga atgccggacc ttccggcgtg acggtggtga tcgcccgcga
     2161 tgacatgatc gctcggactc ccgagaacct gcccgtgatg ctcgattaca agaccctcgc
     2221 tgagagcggt tccctgcaca acacaccgcc ttcctttgcc atctacatgg tgggactggt
     2281 cttcaaatgg gcgaagaagg tgggtggttt ggctgccatc gaaaagatca accgcgaaaa
     2341 agccggcatc gtctacaacg ccatcgacca aagcggcggt ttctatcgcg gacacaccgt
     2401 gcccgaagct cgctcgttga tgaacattcc gttccgcttg ccgagcgaag aactcgaaga
     2461 gaccttcgcc aaagaagcca agaagaatga cctgatcggt ttgaagggtc atcgttctgt
     2521 gggcggtatg cgcgcttcgg tttacaatgc catgagcatt gaaggcgcga aagaacttgc
     2581 caagttcatg gcagagttcc agaagaagaa cggttagagt tcgtacagat tggaattaaa
     2641 agacctccga attttcggag gtctttttct attaagagtc atctcaaaaa tgtcatcctg
     2701 agcaatagcg aaggatctct accattgtgt aagagaccct tcggtcgcga agaacgcttc
     2761 ctcagggtga cagggactag ggattttgta cttctgtgag aatgttattt tctatcttga
     2821 agtacttcga gacacctagt gaaggcggtg ttgtaaacgg ttcgccggga tttctatctg
     2881 catagttgac ggtaatgacg ccattctcaa tgattgtatt ttgtggggca atgcggtccc
     2941 cgaggaagat ggcgttcgag ccttccgtcc ctgaggcagt ttgcaaagca acgacgatat
     3001 agaagaacgt tccacttccg ccagtatctt gcgtgagcag aaaggcgaca tcgctttttc
     3061 catccttatt aaggtcaccg atcgcttcat tgccgaaata acgagtggtg atcttgctcg
     3121 tggacccggg cgcgatttcc acttcaaaga tgccatctgc aagcgttacg aatgaacctt
     3181 cgattaagta ggtggcattt ttataatcag gcaatacgag ggcgggctct gtaatcgggt
     3241 aagcttcgga tacaacttca ggcggtacag ttggggttcc gcaagcgagt aacatcacag
     3301 acaagcataa ggttataaaa attttcttca tggagcgttc ctttattttg agagcgtata
     3361 gccacttacg taatccacga tgaattgcat gggacggtca tgctttggag acttagatag
     3421 atcgtaaata ttaagcatga actgcatggg gtattgcggc gattgttgaa tggtgcggat
     3481 cttctgatcg ttgatgtaaa aatggacttc gcgttccgtc cactcggcgg cgtaggtgtt
     3541 gaattgggtc acgtctaggg agaattcttc ttcgtagaat tcatccttaa tcgatgggtc
     3601 accgaaggga tggacaccat agccgatgag ggcacttttc tttttgatat tgtatccctt
     3661 tagttcaaag atgcagatct ctgccgaata ctcaggcttg tcttcaaagc cgatcatcca
     3721 cagag
//
LOCUS       DAOREF010000185         3723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15253_length_3723_cov_5.971101, whole genome shotgun sequence.
ACCESSION   DAOREF010000185 DAOREF010000000
VERSION     DAOREF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15253_length_3723_cov_5.971101"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKI33_05100"
     CDS             <1..390
                     /locus_tag="PKI33_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36414.1"
                     /translation="LTFPEPVAEPKVEETPAVVAEPVVTETAPVVETPVVEKPAEKKK
                     EPKRVIEEEEEDGEVSKDGVNLDELFQMKPEMFQAGEGADEESDDKKKGKKVKKKGVA
                     LEFDENLGEVVSRKKHKRSDGGDDEAW"
     gene            435..734
                     /locus_tag="PKI33_05105"
     CDS             435..734
                     /locus_tag="PKI33_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNM36415.1"
                     /translation="MVKKPVQRVKHIPQRTCVGCREILSKRQLLRIVRTAEGVRVDPT
                     SKLAGRGAYLHDKRSCWARGLKGGLAHALKTEISAEDRARLEEFMNTLPEAETDG"
     gene            819..2588
                     /gene="infB"
                     /locus_tag="PKI33_05110"
     CDS             819..2588
                     /gene="infB"
                     /locus_tag="PKI33_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNM36416.1"
                     /translation="MSENGNKIELPASIVVRDLAQKIEKSPIDLIKKLMTNGVMATIN
                     QTVDYDTAAIVVAEYGFEAVPEVVEEESKEEAGEVPMWRQMIAGEDKAQLKQRPPVVT
                     ILGHVDHGKTSLLDAIRSTDVAAGEAGGITQHIGAYQVEMKGRLITFLDTPGHAAFTQ
                     MRARGAQGADIVILVVAADDGVMPQTKEAIAHAKAARVPIVVALNKVDKPNANPDRVK
                     QQLAELELVPDDWGGNTMVVPVSAKQKKGIDDLLEGVLLVADSNEIKANPSGKVIGTV
                     IEAELDKSKGVLATLLVQNGTLESGDVVVAGTAHGKLRAITDYKGKPVKKAGPSTPVA
                     VMGLSDVPSAGDLFEVVPSEKEARAIVAERMEVLKAQAQSRKKLSLEDLFSKVQAGEA
                     KELNLIVKADVQGSLDPIITELNKLGQGEIGLKVLHAETGNIGNNDVMLASASKAIMI
                     GFNVQADVDARRMAEKEGVDIRLYEIIYRMTEDIEKALNGMLEPDLKEKVLGKATVLQ
                     VFTASKFGRVAGCRVTDGELKRGAKVRLYRDSDIIYEGDLSSLRHEKDDVKEIRQGFE
                     CGVGFKNFSDIQSGDTLVCYILE"
     gene            2653..3003
                     /gene="rbfA"
                     /locus_tag="PKI33_05115"
     CDS             2653..3003
                     /gene="rbfA"
                     /locus_tag="PKI33_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNM36417.1"
                     /translation="MPSGIRIKRIEDRIQQELSEMLIHEISDPRLKQIYVTGIKVDRE
                     LAFAEVYVSAVEGASRSEEVLAGLESASGFLRRTLSARVELRSFPKLRFHWDPTPENA
                     DHIEKLLAGLRNKK"
     gene            3043..>3723
                     /locus_tag="PKI33_05120"
     CDS             3043..>3723
                     /locus_tag="PKI33_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNM36418.1"
                     /translation="MTDQISGDIKNRLADAKKVVIASHVRPDGDAIGSLLGLGLALIE
                     AGKTVEMILVDGAPASFKFLEGSELIKKEPTGGHDTFITVDCADFKRTGKIFESFGQP
                     DINIDHHKTNEKFGKLNLIEAEEVATAAILTNHLPTWGLKITKPVAAALLTGILTDTL
                     GFRTSNVTPEALRQAATLMESGVDLPEIYMQSLVRKSFPAAKYWGAGLSSLESKDGIV
                     WGTLTLEDR"
BASE COUNT         1046 a          887 c          981 g          809 t
ORIGIN      
        1 ctcaccttcc ccgaaccggt tgctgaaccc aaggtggagg aaaccccggc agttgttgca
       61 gaacccgttg tgacagaaac ggctccagtg gtagaaacac ctgttgtgga gaagcctgcc
      121 gaaaagaaga aagaaccgaa acgcgtcatc gaagaagagg aagaagatgg cgaagtctcg
      181 aaggacggcg tcaacctcga cgagctgttc caaatgaagc cagaaatgtt ccaagctggc
      241 gaaggtgcgg atgaagaatc cgacgataag aagaagggca agaaagttaa gaagaagggt
      301 gttgccctcg agttcgacga aaaccttggc gaagttgtct cacgcaagaa gcacaaacgc
      361 agtgatggcg gcgacgacga ggcatggtag ttaatgattc ccgcctccat aatggaggcg
      421 gggagtaaac ggacgtggtc aaaaaacctg tacaacgcgt aaaacatatt ccgcagcgca
      481 cctgtgtcgg gtgccgggaa atcctgtcga aacgacaatt actgagaatt gtccgcacag
      541 cagaaggcgt gcgtgtggac cccaccagta agttggcagg aaggggcgct tacttgcacg
      601 acaagcgctc ttgttgggcg cgcggattaa aaggcggttt agcccacgct ttgaaaacgg
      661 aaattagcgc agaagaccgc gcccgtttgg aagaatttat gaacactctg ccagaagcag
      721 agactgacgg ttaagccatg tgaacgaata agccactcgc tcacaggctg cctgatacat
      781 tgaacaaaga tatgttttga gattcaagga ggtggcttat gagtgaaaac ggaaataaga
      841 tcgaattacc tgccagcatt gtagtacgag acctggctca aaagatcgaa aaaagcccga
      901 ttgacctgat caaaaaattg atgaccaatg gtgtcatggc aaccattaat cagacggtgg
      961 attacgatac cgccgcgatt gtggttgctg agtatggctt tgaagccgta cccgaagtcg
     1021 ttgaagaaga atcaaaagaa gaagcgggcg aagtgcccat gtggcgtcag atgatcgccg
     1081 gggaagataa ggctcaactg aaacagcgcc cccccgttgt gaccattctg ggtcacgtcg
     1141 atcacggcaa aaccagcttg ttagatgcca ttcgttccac agatgtggct gctggtgaag
     1201 ctggcggtat tacccaacac atcggcgcgt atcaagtgga aatgaagggg cgtctcatca
     1261 ccttcctcga tacgcccggt cacgccgcgt tcacgcagat gcgcgctcgt ggtgctcaag
     1321 gtgctgatat cgtcatcttg gtagtggctg ccgacgatgg cgtgatgccc caaaccaaag
     1381 aagccattgc gcacgccaaa gcggcgcgtg tgccaatcgt ggtggcattg aacaaggtcg
     1441 ataaacccaa cgccaaccca gaccgtgtaa aacaacaact tgccgaactc gaactcgtcc
     1501 ctgatgattg gggcggcaat accatggtcg tacctgtttc tgctaagcag aaaaaaggca
     1561 tcgacgattt gctcgaaggc gttttgctcg tggcagatag caacgagatc aaagccaacc
     1621 ccagcggcaa agtaatcggt accgtcatcg aagcggaatt ggataaatcc aagggtgtgc
     1681 tggcaacctt gctcgtccag aacggtacgc tcgaatcagg cgacgtcgtt gtagctggaa
     1741 ccgcgcatgg caaattgcgc gccatcaccg attacaaggg caagccggtc aagaaggcgg
     1801 gtccttccac gccggttgct gtgatgggtc tcagtgacgt tccctccgct ggagatttgt
     1861 tcgaagtcgt cccgtctgaa aaagaagcgc gtgccattgt ggctgaacgt atggaagtct
     1921 taaaggctca ggcacaatct cgcaagaaac tctcactcga agacctgttt agcaaagtcc
     1981 aggcaggtga agcgaaagaa ctcaacctca tcgtcaaagc cgatgtgcaa ggttcgcttg
     2041 accccatcat tactgagtta aataagctcg gtcagggtga aattgggttg aaagtgttac
     2101 acgccgaaac aggtaatatc ggcaacaatg acgtaatgct cgcttccgcc tcaaaagcca
     2161 tcatgattgg tttcaacgtc caagcagacg tggatgcccg ccgcatggct gaaaaagaag
     2221 gcgtggacat tcgcctttat gagatcatct accgcatgac cgaggatatt gaaaaagccc
     2281 tcaatggtat gttggagccc gatctcaagg aaaaggttct tggcaaggca accgtcttgc
     2341 aggtctttac cgcctcgaag tttggacgtg tggcaggctg ccgtgtaacc gacggcgaat
     2401 tgaagcgtgg tgccaaggtt cgcctttacc gcgactcaga tatcatctac gaaggtgacc
     2461 tctcctctct tcgccacgaa aaagacgatg tgaaagagat ccgccaaggg tttgaatgtg
     2521 gtgttggttt caagaacttc tccgacatcc aatctggcga caccctcgtt tgctacatct
     2581 tggaataagt acttttacta cttacatatc acgtatcaaa gtgatatgta agtagtaaca
     2641 tttggaaaga ctatgccatc tggaattaga atcaaacgaa ttgaagatcg tattcagcag
     2701 gaactttcgg aaatgctgat tcatgagatc agtgatccgc gtctcaagca aatttatgtg
     2761 accggcatca aagtggaccg tgaacttgcc tttgcggaag tgtacgtctc tgccgtggaa
     2821 ggggcttccc gctctgaaga agtgctggca gggctagaat ccgcttctgg gtttctgcgc
     2881 cgcacccttt ctgcccgtgt agaactacgt tcattcccaa aattacgatt tcattgggat
     2941 cccacacctg aaaatgctga ccatatcgaa aaactgttgg cagggttaag gaacaaaaaa
     3001 taatcatcct tattgaaaat aaggaattgt ggagaaatta tagtgacaga tcagatctca
     3061 ggggatataa aaaatcgact cgcagatgct aagaaggtgg tgattgcatc gcatgtacgc
     3121 ccagacggag acgccatcgg ctccctgctc ggacttggac tggctctaat cgaagcaggc
     3181 aaaaccgtcg aaatgatcct ggtagatgga gcgcccgctt ctttcaaatt tcttgaaggg
     3241 agtgaactca ttaaaaaaga acccaccggc ggacacgaca cttttattac tgtagattgt
     3301 gccgatttca aacgaacggg gaaaatcttt gaaagctttg gtcagcctga tatcaatatt
     3361 gaccaccaca aaaccaacga aaaatttggg aaactaaatc taattgaagc agaagaagtt
     3421 gccacagcag ctattctcac caaccacctc cccacttggg ggctcaagat caccaagccg
     3481 gttgccgctg ctttattgac cggtattctg accgataccc ttggtttccg cacatcaaac
     3541 gtcacgcctg aagcgttacg tcaagctgcg actttaatgg aatcgggtgt ggaccttcca
     3601 gaaatttaca tgcaatcgct agtgagaaaa tccttcccgg ctgccaagta ttggggcgcc
     3661 gggctttcaa gccttgaaag caaagatggc attgtttggg gaacgctgac attggaagac
     3721 cgt
//
LOCUS       DAOREF010000186         3721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15269_length_3721_cov_3.320240, whole genome shotgun sequence.
ACCESSION   DAOREF010000186 DAOREF010000000
VERSION     DAOREF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15269_length_3721_cov_3.320240"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1182
                     /locus_tag="PKI33_05125"
     CDS             85..1182
                     /locus_tag="PKI33_05125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM36419.1"
                     /translation="MKIAYVTINVAPKIMLGGVGAKIKRHISIWKDMCHQVRLFSLTP
                     ETISFPDSDQFVFAPFLSLPLLKGFSLEYSRSRKLIEVLHAIEAYQPDIIYFRFGLFT
                     FPLQRIFHIAPVFMEVNSNDVEEYKTRGAFFYWLNRITREIMFRYSAGLLPVSQEIAE
                     LNTKYNRPTCVVSNGIDLDNFIPLAAPKNKAPVLTAIGSPGNWQGMDKLVPFARKYSD
                     LTINIIGDSPQDFSEPIPDNLHIHGFLDKDAIRAIFSNTDAVFSQLGLHRKNMKENPT
                     LKVREALAYGLPLILAHYDADLNDLDTDCILRIPNTEDNLTSHGDAVHEFAYHVMGKR
                     IERELIASRIDQRKKEEKRLAFFEQVLAGKK"
     gene            1182..2333
                     /locus_tag="PKI33_05130"
     CDS             1182..2333
                     /locus_tag="PKI33_05130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM36420.1"
                     /translation="MPYSIVLLGTQMAVGGAQKLLLEQALWFQQHGNRVTVLFFYDRD
                     NLHERWTKTYPFEIYNLEAFDKKAGGLRSLPKLLGGLLKLWQVLKHGKFDAIITFTHD
                     SNLLGMPLAWLAGIHARVGTHLGEIRGMSKWRDGLHTFLVNHGVIQTLVASSARTRNN
                     AIEVGVHPERITTIYNAIMPFDVSSINRESVREKLGLKKDEIFFLAVGRLVYEKGHEF
                     FIEAMAKVVKDDTRAIAGICGAGPLQGELQSQIEKLNIQANMKLLGQWDSIPEILAAA
                     DVFVLPSRWEGLPMALLEGMMAGLPVIATRVEGVDEVVQPGEHGLLVPLESPAELAQA
                     ILQLLRSPQDRQRMGAAARERVLSSYTTDRMCEAYLQVIEQGLGVGKAG"
     gene            2358..>3721
                     /locus_tag="PKI33_05135"
     CDS             2358..>3721
                     /locus_tag="PKI33_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36421.1"
                     /translation="MKRSSIDFGKLIAITIAAWILVEACVEFYNVAWGTGEAVGLFSP
                     RWLILFVSFVLSCVFVYVCVGIAVLDRERFFALIQNIVTLRNRIPILSWALAILFLTI
                     PAWFLQRTMWGVVFDGIYFRLFLWVLTLFLVSFLITRGTTLIEWKSFLVVLILTSSVF
                     TIAISLQGVTDYPFSMGWSEGNRIWDYSMRFGRDRYGIADNQDIYVLLDSGRMLIGGL
                     PFLIPNVTIEIVRLWIGLTLILPYFLVGIAAYIVAEKNVRIWILMTLWVFLFLRQGPI
                     HSPLVLAAALTIFTWRKPLWLAIPLIIYAGYFAQASRFTWLFAPGLWIGMLELAGASL
                     SNGKLNRAHWVRAITLGLSGAFGGFILPKLLPLINIASVATTPVDVTEQIANVGVNSE
                     FVTYAVTDQPLLWYRLLPNSTYGTGILLGLVIAVLPIILWLVWLSASKKWELNGWQKL
                     AIVGP"
BASE COUNT          918 a          732 c          893 g         1178 t
ORIGIN      
        1 gaattctcat cgcgccggga aaagcgatat tggttttgct ttgtggcgtt ggatcaacct
       61 tgagctttgg ttgcgggaat atgtatgaag attgcctatg taacaatcaa tgttgcgcct
      121 aagatcatgt tgggaggcgt gggcgctaag atcaaacgac atatatccat atggaaggat
      181 atgtgccatc aggtaaggtt attctcatta acgcctgaaa ccatttcttt ccctgattca
      241 gaccaatttg ttttcgcccc attcttaagc ctgcctcttt taaagggttt ctctttggag
      301 tattcccgtt cgcgcaaact tattgaggtg cttcatgcca ttgaagcata ccagccagat
      361 attatttatt ttcgctttgg gttgttcact ttccctttgc agagaatttt ccatattgct
      421 ccagtgttta tggaagtgaa ttcaaacgat gttgaggagt ataaaacacg cggggccttt
      481 ttttattggc ttaatcgtat tacccgtgaa atcatgttcc gctattcagc tgggctactt
      541 ccagtaagtc aagagatcgc agaactcaat acgaagtaca atcgcccgac ttgtgtggtt
      601 tcaaatggta ttgatttgga taattttatt ccattagcgg cgcccaaaaa taaagctccg
      661 gtattaactg ctattggctc gccgggcaac tggcaaggga tggataaact agttccattt
      721 gcacgtaaat attcggattt gacgattaat attattggag actctccaca ggatttttct
      781 gaacccatcc ctgataatct gcatattcac ggttttcttg ataaagatgc aattcgtgca
      841 atattttcaa atactgatgc ggtgttcagt caacttggct tgcatcgaaa aaatatgaag
      901 gaaaacccga cgctaaaagt gcgtgaggca ttagcctacg gacttccatt gatacttgct
      961 cattacgatg ctgatctgaa tgacttggat acggattgca tcttaagaat tccaaacaca
     1021 gaagataatc tgacgtctca tggggatgct gtgcatgaat ttgcatatca tgtaatgggt
     1081 aaaaggattg aacgtgagtt aattgcctca agaattgacc agcgcaagaa agaagaaaaa
     1141 cgtcttgcat tctttgaaca agtcctcgcg ggaaagaaat aatgccatat tccatcgtcc
     1201 ttttagggac tcaaatggcg gttggcggcg ctcagaaact tttactggaa caggcgcttt
     1261 ggtttcaaca gcatggaaat agagtgaccg tcctcttttt ctatgatcgc gacaacttgc
     1321 atgaaagatg gacgaagacc tatccttttg aaatttataa cctggaagcc tttgataaaa
     1381 aagctggcgg acttcgcagc ttgcctaaat tgctgggcgg attgttaaaa ttgtggcaag
     1441 ttctcaaaca tgggaaattt gatgccatca ttacctttac gcatgatagc aatctgttag
     1501 gtatgccact ggcgtggctg gcagggattc atgcccgtgt aggcacacat cttggcgaaa
     1561 ttcgtggcat gtcgaagtgg cgtgatggat tgcatacctt tcttgtcaat cacggagtga
     1621 ttcaaacact tgtggcatct tcagcgcgga cccgcaataa tgccattgaa gttggtgtac
     1681 atcctgaaag aatcactact atttacaatg ccatcatgcc ttttgatgtg tcaagtatta
     1741 atcgtgaatc tgtacgcgaa aagctgggat tgaaaaagga tgaaatcttt tttctagctg
     1801 tcgggcggct tgtctatgaa aaggggcatg agttctttat tgaagctatg gctaaagtgg
     1861 tgaaagacga tactcgtgct attgctggca tttgtggagc aggtccgttg cagggagaat
     1921 tgcagtctca aatcgagaag ttaaatattc aagcgaatat gaaactgctc gggcaatggg
     1981 actccattcc agaaatcctt gcagccgcag atgtttttgt tttgccatca cgttgggagg
     2041 ggctcccaat ggcattgctt gagggcatga tggcagggct gcctgtcatt gctacacgag
     2101 tggaaggtgt agatgaagtt gtccagccag gtgaacatgg actattagtg ccgcttgagt
     2161 ctccagcaga actggcgcag gctatattac aattgctccg ctcacctcaa gaccgtcagc
     2221 gcatgggagc ggcggcgaga gagagagtat taagctctta cactaccgat cgcatgtgtg
     2281 aagcgtattt gcaggtgatc gaacaaggct tgggtgtggg aaaagccggg taagcgtaat
     2341 caagcagagg cttaaggatg aagcgttcgt ctatcgattt tggaaaacta attgcaatta
     2401 caatcgctgc atggatactc gttgaggcat gtgtcgagtt ctataatgtg gcgtggggca
     2461 cgggcgaggc ggtggggtta ttctcaccac gttggcttat cctttttgtt tcttttgttt
     2521 tatcatgtgt ttttgtatat gtatgtgtgg gcattgctgt tcttgatcgc gaaagatttt
     2581 tcgctttaat tcaaaatata gttacattac gaaatagaat accaatctta agctgggctc
     2641 ttgccatttt atttttgacc atcccggcat ggtttttgca acgaaccatg tggggtgttg
     2701 tttttgatgg catttatttt cgtttgtttt tatgggtatt gacgcttttt cttgtatctt
     2761 ttcttattac tcgcggaacc acattgattg aatggaagtc attcctcgtc gtattgatac
     2821 tgacctcaag tgtttttaca atcgcgattt ctttgcaagg tgtaacagat tacccattct
     2881 ctatgggttg gtctgaaggt aatcgtattt gggattattc aatgcgcttc gggcgtgatc
     2941 gttatggcat tgctgataac caggatattt atgttctgct tgattctggg cgtatgctaa
     3001 taggtggttt gccattttta attcccaatg tgactattga aatagtgcgc ctatggattg
     3061 ggcttacact gatcctgccg tattttctgg ttggaattgc cgcttatatt gttgctgaaa
     3121 aaaatgttcg tatttggata ttgatgactt tatgggtctt cctgtttttg cgacagggtc
     3181 ctattcattc tccgttagtt cttgctgcgg cattgactat cttcacctgg cgtaaaccat
     3241 tatggcttgc aattccattg attatctatg ctggatattt tgcccaagcg agtcgattta
     3301 cctggttgtt cgcacctggt ctatggatcg gaatgcttga attagcaggc gcttctttat
     3361 ctaacggaaa actgaatcga gcccattggg ttcgggctat cactttaggc ttgtccggcg
     3421 catttggcgg attcattttg cccaaactgt tgccattgat taatattgcg agcgttgcca
     3481 caactcctgt ggatgtcacc gaacagattg ccaatgtcgg ggtcaactca gaattcgtga
     3541 cctatgcagt gaccgaccag cccctcttgt ggtatcgtct acttcccaac tcaacctatg
     3601 gaactggtat tttgcttggg ctggtcattg ccgtattgcc gattattctt tggttggtct
     3661 ggctttctgc ctctaaaaaa tgggaattaa atggttggca aaaactagcc attgttggac
     3721 c
//
LOCUS       DAOREF010000187         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15294_length_3717_cov_5.023484, whole genome shotgun sequence.
ACCESSION   DAOREF010000187 DAOREF010000000
VERSION     DAOREF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15294_length_3717_cov_5.023484"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PKI33_05140"
     CDS             complement(<1..881)
                     /locus_tag="PKI33_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36422.1"
                     /translation="MKKFVTLYMPILGVLIGNIILLGVAGKMSSISVQQVVTPFSFSV
                     SLLVLVTGLLPALVRPQSNHALVACFSSLFAFFLLIPFELDDLPTIHPGVPLVSFLSP
                     YFALRIMQAAILLPMALHVSVRFPRVIETPQRNILNAYIFSILLLLAFFYSKVMWQRV
                     FTIGAFFLWFTYVIIFFFRNLLHVARDPAPDNVLEAQRARVVLFSMLLAEVPLWLRPL
                     TLALGLEIFSYNLLLVFQLFVPLGIAYAVLRHDLFGIDRALRRTLVYGSVSLLLLTLY
                     LALTTGITLLFESSLTT"
     gene            complement(931..2268)
                     /locus_tag="PKI33_05145"
     CDS             complement(931..2268)
                     /locus_tag="PKI33_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36423.1"
                     /translation="MISTDNNNARRDWTLLIFIIPIGIILIIFVGQLAVRLLPFWRVD
                     ADMNSNLQPDSSSARPLALLEPILPEILTPMAWAENYLTPGSAVNFPPFLTFEPSSTP
                     VVASVTTTSISPTSTTATPTSSPVPTGSVTTQPPNGGGGSSTPPPPTTCQDPSADNFG
                     GALPCDYTSTTTCQDHAADNFGGTLPCDYTSTTTCQDHAADNFGGALPCDYTSTTTCQ
                     DINSNNYGGYLPCDYGLISTINPGYGTPVVPIPSQIGIGTLPDNNDPNSDANIGLIDT
                     GTYIVVNLIIVVGTTPDNSYDLIYYEYNNNGFVNLDWIIIGISNYADGSQYYEVFNWG
                     NGTPDTNSNVGDVAQLSGTELDNQSIPVSGNTEVPPSQTELYDPDYSPGTPETNGPLP
                     QSGILIDVDSATSSPPPNTYNYVVIIAPLGDSDGAVQVDAVQAVEVPIPTPTP"
     gene            complement(2265..>3717)
                     /locus_tag="PKI33_05150"
     CDS             complement(2265..>3717)
                     /locus_tag="PKI33_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM36424.1"
                     /translation="PNPNDPPRRALGGNLVNNEVYYYTALPIAVDGKFNGVIVLGTSI
                     QTLLPAFKQFALADIILYGNGGQAISTTIGSVDDEMLSKLNIPEDQYKELIESDGPLV
                     TGINTEIVGRNFSIGRAPLQIGNDRIGVFAVALPTDFVTQFGSNNRTAYALVFSGLML
                     VVIVIGYFVSRMIIVPLYSLVNTSQAIASGDLNRRTGLQTKDEIGKLASTFDEMTARL
                     QERTIELEEKNETLRKMDKTKTNFIQISAHELRTPLTLIMGYSQMLEQDLKDNPDMQS
                     LAHGILEGAERMTDVVDSMLDVSRIDSNSLVLRKTSMQVEPVVRKVQKEFETAFEERK
                     INFQTDGLNTLPAIIADPDMLQKVFYHLIMNAIKYTPDGGDVKVLGKYVNGTEPPQLE
                     ISVCDTGIGVDPSMQDLIFQKFHQTGEVLLHSSGKTKFKGGGPGLGLAIARGIIQAHG
                     GRIWVESPGHDEKNFPGSKFVISLPAQKKESQL"
BASE COUNT         1181 a          778 c          786 g          972 t
ORIGIN      
        1 cttgtggtga gagaagactc aaagagtaaa gtaatgcctg tggtaagagc caggtataac
       61 gtcagaagaa gaagcgaaac agacccatag accagagtcc gcctgagggc gcggtcaatg
      121 ccgaagaggt cgtgtcgtaa tacagcgtag gcgattccca aaggcacaaa aagttgaaac
      181 accaaaagca ggttataaga aaaaatttct aagccaagtg ccaaggtcag cgggcgcagc
      241 cagagcggta cttcagcaag taacatacta aaaagcacaa ctcgtgcccg ttgagcttcg
      301 agaacattat caggcgcagg gtcgcgtgca acatgcagca agttccgaaa gaaaaaaatg
      361 atgacatatg tgaaccataa gaagaaagca cctatggtaa aaactcgctg ccacatgact
      421 ttactataga aaaaagcaag cagtagcaga atagaaaaaa tataagcgtt taagatattt
      481 cgctgtgggg tttctataac tcgcggaaac ctcacactca catgcaatgc cataggcaaa
      541 agaatggctg cttgcataat ccgtaaagcg aagtaaggac tcaaaaaaga gacgagaggc
      601 actccggggt gaatagtagg aagatcatca agctcaaaag gaatcagcag aaaaaaagca
      661 aagagacttg aaaagcaggc aactaaggca tgatttgatt gagggcgaac gagtgcaggt
      721 aataaacctg ttactaaaac aagaagtgaa accgaaaacg aaaatggggt tacgacttgt
      781 tgcacagata ttgaactcat cttcccggca actccaagaa gaataatgtt tccaatcaaa
      841 acacccagaa taggcatgta aagagtcaca aactttttca tgttctttga ttataaataa
      901 aagagaccgg ttagaccggt ctcttttgta ctatggagta ggagtgggta ttggcacttc
      961 aactgcctgg actgcgtcga cttgcacagc accgtccgag tcacctaatg gtgcaataat
     1021 tacaacataa ttatatgtat ttggtggtgg agaacttgtt gcagaatcaa catctatcaa
     1081 tataccggat tgcggaaggg gtccatttgt ttccggcgtt ccaggactgt agtctgggtc
     1141 atacaattca gtttgagaag gcggaacctc ggtatttccc gagactggta tggattgatt
     1201 atctaactca gttccgctta attgtgcaac atcgccaacg ttcgaattag tatctggagt
     1261 cccattcccc caattaaaaa cttcataata ttggcttcca tctgcataat ttgaaattcc
     1321 tataataatc cagtcaagat tcacaaatcc attattattg tattcataat atatcaaatc
     1381 gtagctatta tcaggggtag tacccacaac aattattaag ttaacaacta tatatgttcc
     1441 agtatcaatc aaaccaatat ttgcatcgct attcggatca ttattatcag gaagggttcc
     1501 tattccaatc tggcttggaa tgggaacaac aggagttcca tacccagggt taatcgttga
     1561 aataagtcca taatcacatg gcaaatatcc gccatagtta ttagaattga tatcctggca
     1621 tgtggtagta gaagtgtaat cacaaggcaa agcgccacca aaattatcag cagcatggtc
     1681 ttgacatgta gtagtagatg tgtaatcaca gggcaatgtt ccaccaaagt tgtcagcggc
     1741 atgatcctga catgtggtag tagatgtgta atcacagggc aacgcgccgc caaagttatc
     1801 tgctgaagga tcttggcaag tcgttggtgg gggtggagtg ctactacccc caccgccgtt
     1861 aggcggttgt gttgtaacac tccctgtagg tacaggtgaa gatgtaggtg tagctgttgt
     1921 gctagttgga gatatggatg ttgttgtaac cgacgcaaca accggcgttg aactgggttc
     1981 aaatgtaaga aaaggtggaa aattaacagc agagccagga gttaagtaat tttctgccca
     2041 agccatcggg gtaaggattt ctggaagaat tggttcaagt aatgctaaag ggcgtgcaga
     2101 tgaagagtcc ggctgcaagt tagaattcat atctgcatcc actctccaaa atggaagtaa
     2161 ccgaactgcc aactgtccta caaaaataat gagaataata ccaattggaa taataaatat
     2221 aagcaatgtc caatcacgcc gcgcattatt attgtctgtg cttatcataa ttggctctcc
     2281 ttcttctgag ccggtaaaga aataacaaac ttactacctg gaaaattctt ttcatcatgc
     2341 ccagggctct ctacccaaat acgcccacca tgtgcctgaa taataccacg agcaatagcc
     2401 aacccaagcc cgggtccccc ccccttaaat tttgtctttc cagaagaatg aagcagaacc
     2461 tcacctgttt gatgaaattt ttggaagatc aagtcttgca tactggggtc aacaccaata
     2521 cctgtatcac agactgaaat ctctagttgt ggaggctctg tgccattaac gtatttgcct
     2581 aagactttaa catctccacc atctggtgtg tatttaatcg cattcattat aagatgataa
     2641 aacacttttt gaagcatatc tggatccgct attatcgcag gaagagtatt cagaccatcc
     2701 gtctggaagt taatttttct ttcttcaaaa gccgtttcaa attccttttg cactttacgt
     2761 accacaggtt ccacctgcat agaagtcttt cgcaacacaa gagaattact atcaatgcgt
     2821 gaaacatcca acatgctatc aacgacatct gtcatgcgct ctgccccttc tagaattccg
     2881 tgcgcaagac tttgcatatc aggattatct tttagatcct gttccaacat ttgagagtag
     2941 cccataataa gagtcaaggg agtgcgtaat tcatgggctg agatttggat aaaattcgtt
     3001 ttggtcttat ccatctttcg aagcgtctcg ttcttttcct caagttcaat agttcgttct
     3061 tgtaatcgag cggtcatctc atcaaacgta ctcgccagtt tacctatttc gtctttagtc
     3121 tgtaatcccg ttctgcgatt cagatcgcca cttgcaattg cttgtgatgt attgaccaaa
     3181 gagtatagcg gaacaatgat catacgagac acaaaataac caataacaat aacaaccaac
     3241 attaacccag aaaacactag tgcgtaagct gtacgattat tagacccaaa ctgagtaaca
     3301 aaatcagtag gtaaggcgac agcaaaaacg ccaattcgat cattcccaat ttgcagcgga
     3361 gcacgcccaa tagaaaaatt tcgtcctaca atttcagtat tgatcccagt aactagtgga
     3421 ccgtctgact cgattagttc cttgtactga tcttcaggaa tattgagttt tgaaagcatc
     3481 tcatcatcga cagagccaat ggttgtagag atagcttgtc caccatttcc atacaaaata
     3541 atatctgcaa gagcaaattg tttaaatgca gggagcagag tctgaataga cgtgcctagt
     3601 acaattaccc cattaaattt tccatcaaca gcaatgggta acgcagtgta atagtaaact
     3661 tcattattaa caagatttcc gccaagagca cggcgggggg gatcatttgg gtttggc
//
LOCUS       DAOREF010000188         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15304_length_3715_cov_4.496721, whole genome shotgun sequence.
ACCESSION   DAOREF010000188 DAOREF010000000
VERSION     DAOREF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15304_length_3715_cov_4.496721"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKI33_05155"
     CDS             complement(<1..857)
                     /locus_tag="PKI33_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNM36425.1"
                     /translation="MKTLIKNGTLITASDTFNADILIEDEKISRIGLNLEADSDHQVD
                     ATGKLIMPGGVDPHVHLDLPMFDTVSSDDHYTGHKAAAFGGTTTAMDFVVLENEGFEH
                     SVDLWMKKAEKAAIDYSFHMNLTRFDGNIAKTIPSLMKMGIQTLKVFTAYNGRLRLDD
                     GGIFKALQIARDNGMLVMAHCENGDVIETLTTQALAEKRTSPIWHALTRPSWGAVESS
                     LRMAAMAEQADAPVYIVHMNAGGEVDMLQYARERGVKVMGETCPQYLFFTVDHLARPD
                     GAKWICSPP"
     gene            915..1751
                     /locus_tag="PKI33_05160"
     CDS             915..1751
                     /locus_tag="PKI33_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM36426.1"
                     /translation="MNPKRFFVLLTLASLLFFGLWLPAGASSSQQESPPTPTPGTDGR
                     IIYVVQAGDNCFRVAAINGITVEQLRQLNSKLDENCTLTEGQELLIGVAAVATATAPA
                     NVAVGTPTITPTPLSGLTEVCVLLFNDLNGDAMRQDTEPAIAGGAVSVTEANGKYSAS
                     LETVIPSDPTVYQGTCFSNVPEGTYNITMAIPDQYNPTMSLSYSLEVDAGDRAMVDFS
                     AQSKDVVVDQTETEGNAGGASTGLVIFGIVLLLGGAGLGYYAWRSNKPESKLAGGGIL
                     KK"
     gene            complement(1768..2397)
                     /locus_tag="PKI33_05165"
     CDS             complement(1768..2397)
                     /locus_tag="PKI33_05165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNM36427.1"
                     /translation="MIPKPDLTYENEIWSAYQFIAGMDEAGRGALAGPVSVGAVILPL
                     NHSHLLRALSGVHDSKQLTPRKRSELVPQIQATALAHGVGFASAEEIDQVGIVPATRL
                     AASRALEALSLYPQYLLTDFRLELPELDIPQTAIVKGDQRSLSIACASILAKTARDEF
                     MQNLNEKYPEYRLDHHKGYGTLFHRKKIETLGHSPIHRKTFQLKNNREA"
     gene            complement(2381..3112)
                     /locus_tag="PKI33_05170"
     CDS             complement(2381..3112)
                     /locus_tag="PKI33_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM36428.1"
                     /translation="MNTPFLSIIIPAHNEANRLPRTLEQVFAYLKLQNYSSEVVVVEN
                     GSSDNTLELATHFIKEHSNLVVVQEHQKGKGNAVRRGMLVARGEYRFICDADLSMPIE
                     DLGKFLPPQLNDFDIAIGSREAPGAIRYNEPPYRHIGGRAINLAIRFLILPGLRDTQC
                     GFKCFRAETTTRLFEQQTLMGWSFDIELLYLARRKKLHIREIPIHWYFDAGSKVSAVR
                     DALRMISDIFRIHLNALRGTYDSKA"
     gene            complement(3120..>3715)
                     /locus_tag="PKI33_05175"
     CDS             complement(3120..>3715)
                     /locus_tag="PKI33_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36429.1"
                     /translation="EWNSVKNGVSVPASRNLIDLLIKYTRVFQRYLQIHKINIPVQVE
                     PVLIATNPGANIESVRPAVRVVRSDAIKQFATSINQASPVLRAEQVIASADLIIEPHP
                     ASTEKPAEPQIPVERPMSRAQAIFKASEEGDAEQKPKVAAPQPRPRSAQNAQGQAKPP
                     AKKAPAISRRQIFLLVGLGIFECCILFGGIYVLFFLS"
BASE COUNT          935 a          817 c          963 g         1000 t
ORIGIN      
        1 atgggcgggg aacaaatcca ttttgcgcca tcggggcggg caagatggtc aacggtgaag
       61 aagagatatt gcgggcaagt ctcgcccatc actttcacac cacgttcacg ggcgtattgg
      121 agcatgtcca cttcgccgcc tgcattcatg tgaacgatgt acacgggggc atcggcttgt
      181 tctgccatgg ctgccatacg caagctggac tcaacggctc cccaggatgg gcgggtgagg
      241 gcatgccaaa tgggagaggt tcttttttcg gcaagagctt gtgtggtgag ggtttcgatg
      301 acgtcgccat tttcacaatg tgccataacc aacatgccgt tatcgcgcgc aatttgtaac
      361 gccttgaaga taccaccatc gtcgaggcgt agacgtccgt tataggcggt gaatactttc
      421 agggtttgaa tacccatctt catgagcgag gggattgtct ttgcaatgtt cccgtcaaaa
      481 cgggtgaggt tcatgtgaaa ggaatagtcg atggctgctt tttcagcttt cttcatccat
      541 aaatcgacgg aatgttcaaa gccttcattt tctagcacca caaagtccat ggcagtggtt
      601 gtcccaccaa aggcggcggc tttgtgaccg gtgtaatggt catcggaaga aacagtatcg
      661 aacatcggca ggtcgaggtg aacgtgaggg tctacgccac cgggcatgat cagcttacct
      721 gtggcatcca cttgatggtc agaatcagct tcgagattaa gtccgatgcg cgagattttc
      781 tcatcttcga tcaaaatatc cgcgttgaat gtgtcggatg cggtgattaa ggtcccgttc
      841 ttaattagag tcttcatgca agcctcattt cagctttatt ttataccaaa tcgaatctgt
      901 tataattttc ttcaatgaat ccaaagcgtt ttttcgtctt gttgaccctg gctagtttgc
      961 tgttcttcgg gctgtggttg cctgcgggcg cgtcatcgag ccagcaagaa tctcctccta
     1021 caccaacccc tggcacggat gggcgtatta tttatgttgt tcaagcaggc gataattgtt
     1081 tccgtgtggc tgcgattaat ggcatcacgg tggaacaact tcgtcagttg aattcaaagt
     1141 tggatgaaaa ttgtacgttg actgagggac aggaattatt gatcggtgtt gcggctgtag
     1201 cgacggcaac agcgcctgcg aatgtagcag tggggacgcc aaccattact ccaactccgc
     1261 tttctggttt aacagaagtg tgtgtgcttt tgttcaatga tcttaacggt gacgctatgc
     1321 gccaagatac agaacccgct attgctggtg gcgcagtaag tgtgaccgaa gccaatggaa
     1381 aatattctgc ttcattagaa acagtcattc catcagaccc aacggtatat cagggtactt
     1441 gtttttcaaa tgttcccgaa gggacatata acatcaccat ggctatccct gaccaatata
     1501 acccaacgat gtcgttgagc tattctctgg aagtggatgc tggtgatcgt gctatggtag
     1561 atttcagcgc tcaatcgaaa gatgtagtgg tagaccagac tgagacagaa ggaaatgcag
     1621 gtggtgcatc cactgggctt gtcatcttcg gtattgtttt attacttggc ggggctgggc
     1681 ttgggtatta tgcctggcgc tcgaataagc cagaaagtaa attagctggt ggcggcatct
     1741 tgaaaaaata aatgaagacc gcgaggctta agcctcgcgg ttgtttttta actgaaaagt
     1801 ttttcggtga atgggggagt gcccgagcgt ttcgatcttc tttcgatgaa agagcgtccc
     1861 atagcctttg tgatggtcca agcgatattc tgggtatttc tcattcaaat tttgcatgaa
     1921 ctcatcgcgg gcagttttcg ccagaatgga tgcgcaggca atacttaatg agcgttgatc
     1981 tcctttgaca atggcagttt gcggaatatc taactcaggg agctcaagac ggaaatccgt
     2041 tagtaagtac tgcggataaa gagataaagc ttccaatgca cggctcgcag ctagacgtgt
     2101 agctggcaca attcccactt gatctatttc ttcagcggag gcgaatccga ctccgtgggc
     2161 gagggcagtt gcttgaattt gtggaacgag ttcggagcgt tttcggggtg tgagttgctt
     2221 agagtcgtga actccgctca aggcgcggag aagatgcgaa tgattgagag ggagaatcac
     2281 tgccccaacc gagaccggtc cagccagtgc gccgcgtccg gcttcatcca tgcctgctat
     2341 gaattgatac gcggaccaaa tctcattttc gtaggttaaa tcaggctttg gaatcatacg
     2401 tgccacgaag ggcattcagg tgaatacgga agatatcgct gatcatacgt agagcatcgc
     2461 gaacggcact tactttactg cctgcgtcaa aataccaatg aatagggatt tcacgaatat
     2521 gtaatttttt tcggcgagcc aggtagagta actcaatgtc aaaagaccag cccattaagg
     2581 tttgctgttc aaacaatcgg gttgtcgttt ccgctcgaaa acatttgaat ccgcattgtg
     2641 tgtcacgcag acccgggaga atcagaaaac ggattgcaag gttaatggca cgtccgccaa
     2701 tatggcgata gggtggctcg ttataacgta tggcaccggg ggcttcacgt gaaccaatcg
     2761 caatatcaaa gtcattgagc tggggtggga ggaattttcc taaatcttca attggcatgg
     2821 aaagatcagc atcacaaatg aagcgatatt caccacgtgc tacaagcatg ccgcgccgaa
     2881 cagcattccc tttgcctttt tgatgctctt ggacaacaac caaattagaa tgttctttta
     2941 taaagtgggt cgcaagctca agcgtgttat ctgaacttcc attttccaca actactactt
     3001 cacttgagta gttttgcaac tttagatatg caaaaacctg ctcgagagta cgcggcaggc
     3061 gatttgcttc attatgggca gggatgatga tagataagaa tggcgtgttc aaagcgatgt
     3121 tagcttaaga aaaacaggac gtagatgcca ccaaatagaa tacagcactc aaagatgccc
     3181 aaccctacta ataggaatat ttgtctacga gagattgcag gtgctttctt ggcaggaggt
     3241 tttgcctgcc cttgagcatt ttgcgccgag cgtggacgtg gctgtggggc ggcaactttt
     3301 ggcttttgct cagcatcacc ttcttcagaa gccttaaata tcgcttgcgc acgagacatg
     3361 gggcgctcaa ccggaatttg tggctcagca ggtttttctg tacttgcagg atgtggctca
     3421 atgataaggt ctgcggatgc aataacctgc tctgcacgta agaccgggct tgcctgattg
     3481 attgaagtag caaattgttt gatcgcatca ctacgtacta cacgaacagc agggcgcact
     3541 gattcgatat tggcgccggg gttggtcgcg atcaaaactg gttccacctg cactggaata
     3601 ttgattttat ggatttgaag ataacgctgg aaaacacgcg tatattttat taagagatcg
     3661 atcaaatttc ggcttgccgg aacagatacc ccattcttaa cactattcca ttcat
//
LOCUS       DAOREF010000189         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15342_length_3710_cov_3.019152, whole genome shotgun sequence.
ACCESSION   DAOREF010000189 DAOREF010000000
VERSION     DAOREF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15342_length_3710_cov_3.019152"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKI33_05180"
     CDS             complement(<1..738)
                     /locus_tag="PKI33_05180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNM36430.1"
                     /translation="MKNLITKRPMIFSVIVMLILEAFVLAGLFFSRVTGVPLVSLDLP
                     MLMLNAVLAIVILSALRGWREAGFNAPSQWKKMSLFLVPLALLIGPTLFFQPQLPEPA
                     KIVVLIIVTLLIGFQEEAIFRGILLSAFRPQGVMKAVLISAALFGIIHINSLFVGRDP
                     IFVLSQIIASFLGAIGLGALRIRTNTVVPLVLLHAFNDFVQFSATGGMEAGDVALYIP
                     VLKVVISGLMALYGFYLLRGYKEETTSA"
     gene            882..1136
                     /locus_tag="PKI33_05185"
     CDS             882..1136
                     /locus_tag="PKI33_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36431.1"
                     /translation="MNANQRPIFYKIRLRGKLDLEQAAWFPELVHAEDDNGNTLLVGE
                     LPDQAALMGVLFRAHNLNLKILCVKIKTGKTGELKNVQND"
     gene            1120..1452
                     /locus_tag="PKI33_05190"
     CDS             1120..1452
                     /locus_tag="PKI33_05190"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HNM36432.1"
                     /translation="MFKMINLLVRFLLELCILAIFGYWGFQTGSNPFMKFLLSLGTPI
                     LFAVVWGTFLAPKSPMRLHEPWLFLLELVIFALTCWALYSTGKVDLTVAFGGIYILNK
                     ILMVIWRQ"
     gene            1452..2090
                     /locus_tag="PKI33_05195"
     CDS             1452..2090
                     /locus_tag="PKI33_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36433.1"
                     /translation="MNKRNWLAFFGFLYVIVWVIGLLIPTGTLSPSMSNAELQQVLLA
                     NQLARLIQVYLIDGVAGIAILLFAVAAASLFRTVEEKPLARVVLSAGVVAGTISLVQA
                     AVQQTLVNPDLLASAETPFRTLLVLVNQIDTFKLMALALLSISTSMLGVRTRLIPAWV
                     NWLGIVLTLALILGGLSFAFANSLLTAVLFASLPMLLVWVGAVSAVAMRQVK"
     gene            2107..2673
                     /locus_tag="PKI33_05200"
     CDS             2107..2673
                     /locus_tag="PKI33_05200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNM36434.1"
                     /translation="MEQISQNTALIIIDVQKGFDEPVWGERNNPQAEDNIVRLLEAWR
                     KYGRPVFHVQHMSLSPSSPLNPDHPGNAIKDELQPREGESLIQKNVNSAFIGTDLEQQ
                     LRARGCNSLVIVGLTTPHCVSTTTRMAGNFGFETYLVSDATAAFELTDHNGRTYSAQE
                     IHDVTLTTLNGEFAKVIETDMLLRLLED"
     gene            2676..2876
                     /locus_tag="PKI33_05205"
     CDS             2676..2876
                     /locus_tag="PKI33_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36435.1"
                     /translation="MNLVIRPETPQDIAAIYQVNQLAFDRENEARLVDSLRHSGAVTL
                     SLVAQMDNEILSGTSYSRPLQL"
     gene            2873..3190
                     /locus_tag="PKI33_05210"
     CDS             2873..3190
                     /locus_tag="PKI33_05210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNM36436.1"
                     /translation="MNSDGQWAAVALGPMAVLPAHQKQGVGSTLIRTGLKELLALGHN
                     VVIVLGHPEYYPRFGFKHSKPLGIQWEINVPEEVFMVAELSTGALSGRMGIVRYHSAF
                     KDV"
     gene            complement(3254..3346)
                     /locus_tag="PKI33_05215"
     CDS             complement(3254..3346)
                     /locus_tag="PKI33_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36437.1"
                     /translation="MRQLNLSVRAYHRILKLARTIVDLAGSEEI"
BASE COUNT          912 a          992 c          928 g          878 t
ORIGIN      
        1 ggctgaggtg gtttcttcct tgtaaccgcg caacaagtag aaaccgtaca acgccataag
       61 cccagaaatg acgaccttca aaacggggat gtaaagagcc acgtctccgg cttccatgcc
      121 gcctgttgcg ctgaattgca cgaagtcatt gaacgcgtgg agcaaaacca gcggcacgac
      181 cgtgttcgtg cggatgcgca acgcgcccaa gccgatcgct ccaaggaatg acgcgatgat
      241 ctgcgacagc acgaagatgg gatcgcgccc gacgaaaagc gaattgatgt ggatgattcc
      301 gaacaatgca gcggagatca agacagcctt catcacgcct tgcggtctga atgcgctgag
      361 caggattccg cgaaatatgg cttcttcctg aaagccgatc aacagcgtga cgatgatgag
      421 gacgacgatc ttggcaggct cggggagctg cggctggaaa aagagggtcg gtccgatcag
      481 cagcgccaac ggcacgagaa acaggctcat tttcttccat tgggacggcg cattgaagcc
      541 cgcctcccgc cacccgcgca acgcggaaag gatcacaatg gcaagcacgg cattgagcat
      601 cagcatcggc aggtcgagcg agacgagcgg cacgcccgtc actctgctaa agaagagccc
      661 cgccagaaca aatgcctcaa gaatcagcat gacgatcaca gaaaagatca tgggtcgttt
      721 tgtaattagg tttttcattt ttttctcctt gccgcccagt gtaggcgatg tgctttcctt
      781 ttccatctcc caacaggggg atttcggcgg gggagaaact acccccacaa atacctccat
      841 tgggagatga aagaatcaac ccgcctgcat aagatgggga catgaacgca aaccaacgcc
      901 cgattttcta taagatccgc ctgcgcggca aactggacct cgaacaggct gcgtggttcc
      961 cagagttggt ccacgccgaa gacgacaacg gcaatacgct gctggtgggc gagttgcctg
     1021 atcaagccgc gctgatgggt gttctcttcc gcgctcacaa tctcaatttg aagattttgt
     1081 gcgtgaagat caaaactggc aaaacaggag aactcaaaaa tgttcaaaat gattaatctg
     1141 ctcgtgcgct ttctacttga actctgtatt ctcgccatct tcggatactg gggcttccaa
     1201 acaggcagta acccctttat gaagttcttg ctcagcttgg gaactcccat cctattcgcc
     1261 gtagtgtggg ggaccttcct cgcgccgaaa tcgcccatgc gcttacatga accctggctg
     1321 tttctgctcg aactcgtcat ttttgccctg acgtgctggg cgttatacag cacaggcaaa
     1381 gtcgatctga ccgtcgcatt cggcggtatc tacattctca acaagatttt gatggttatc
     1441 tggaggcagt aatgaacaaa agaaattggc tcgcgttttt cggttttctg tatgtgatcg
     1501 tttgggtgat cggactattg atccccacgg ggacgctctc cccgtccatg tccaatgcag
     1561 aacttcagca agtccttctt gccaatcaac tggcgcgttt gatacaggtc tatcttattg
     1621 acggggtagc gggcatagcc atcctgctct tcgcagtggc ggcagccagt ctattccgaa
     1681 ccgtagagga aaagcccctt gcccgtgtgg tcttgagcgc aggcgtggtc gcgggcacca
     1741 tctcactcgt gcaggcggct gtgcaacaaa cccttgtcaa ccctgatctg ctcgcctcgg
     1801 cagagactcc cttcaggacg ctgctggtgt tggtcaacca gattgacacc ttcaaattga
     1861 tggcgttggc gttgctgagc atttccacat ccatgcttgg ggtgcgcact cgcctcatcc
     1921 ctgcctgggt caactggctt ggaatcgtcc tcaccctcgc cctcatcctc ggcggactca
     1981 gtttcgcctt tgccaactca ctgctcactg ctgtcctgtt cgcttccctg cccatgttgc
     2041 tcgtgtgggt gggtgcggtc agcgctgttg caatgagaca ggtaaaataa ggtggaagga
     2101 caatatatgg aacaaatttc tcaaaacacc gccctgatca ttatcgacgt tcaaaaaggt
     2161 tttgatgaac ctgtctgggg cgagaggaac aatccgcaag ccgaagacaa tatcgtcagg
     2221 ttgttagagg cgtggcgtaa atacggacgc cctgtcttcc atgttcaaca catgtccctc
     2281 tctccaagtt caccattgaa tccagatcat cccggtaacg ccatcaaaga tgaattacaa
     2341 ccacgtgaag gtgaatcgct gattcagaaa aacgtcaaca gcgccttcat cggcacagat
     2401 ttggaacagc aacttcgtgc gcgcggatgt aactcgctgg tcatcgtcgg tctcaccacc
     2461 ccgcactgtg tctccacgac gacgcgcatg gcaggcaatt ttggctttga aacctacctc
     2521 gtctcagacg ccaccgccgc cttcgaactc accgaccaca acgggcggac atattccgca
     2581 caagagatcc acgatgtaac cctaacaacc ctgaacggag agttcgccaa agttatagaa
     2641 acagacatgc tgcttaggtt gttggaggat tgacgatgaa ccttgtcatt cgccctgaaa
     2701 caccgcaaga tattgccgcc atttatcagg ttaaccaact ggcgttcgac cgcgagaacg
     2761 aagcgcgcct cgttgactct cttcgtcata gtggagcagt aaccctgtca ttggtcgcgc
     2821 aaatggacaa cgagattttg tcgggcacat cctattctcg cccgttacag ttgtgaatag
     2881 cgacggtcaa tgggctgctg tcgcgttggg tcccatggct gttttgcccg cccatcaaaa
     2941 acaaggggtc ggctccacat tgatccgcac cgggttgaag gaacttctag ctcttgggca
     3001 taatgttgtc attgttctgg ggcatcccga gtattacccg cgttttggtt tcaagcattc
     3061 caaacccctt ggtattcagt gggaaatcaa tgtgccagaa gaagtattca tggttgcaga
     3121 attaagtacg ggagcgctaa gcggtaggat gggtattgtt cgataccatt cagccttcaa
     3181 agatgtgtag aggcaatcaa gtcgccccat tcaacttcgg acgatactgt agtgcttcct
     3241 ctaggttcgc ggactatatc tcttcactcc ccgccagatc cacgattgta cgtgcaagtt
     3301 tgaggatgcg gtgataggcg cgcaccgaca aattgagttg cctcatgcgc agcacgattt
     3361 cgccgcctgc atcaagctct gatgttgttt gatgagcggc attcctgcag ggaattgatc
     3421 cacaatggag tagatatggg taacatttaa tgactcttca atcaaaaatt aattgcccca
     3481 aaacatcgtc taaggtatca tgtagttcca agagaataca tcaaccgaaa ggaaatttgc
     3541 gtaaaacggc aatatcttcg actaaaaacc agccatttta tgtataaaaa tgccatgaaa
     3601 ccttcctctg ccaccgccat cagccacccc aatatcgcat tggcgatgta atctaaaggg
     3661 aacacagtat ttagtagcag caaggttggt tttacctaaa tgtgttcaca
//
LOCUS       DAOREF010000190         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15396_length_3702_cov_3.927612, whole genome shotgun sequence.
ACCESSION   DAOREF010000190 DAOREF010000000
VERSION     DAOREF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15396_length_3702_cov_3.927612"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..593
                     /gene="ruvX"
                     /locus_tag="PKI33_05220"
     CDS             174..593
                     /gene="ruvX"
                     /locus_tag="PKI33_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNM36438.1"
                     /translation="MRILAVDHGEKRIGLALSDPTATIASPLKVIQHVSRLIDAAQVA
                     EIAMQNEVGLIVVGQSYDEEGDPNPAGRRAGRFADELRNQTNIPIEMWDESHSTQIAR
                     AARIELGVSRKKRAGHQDEFAAVVILQSYLEAKRNSE"
     gene            590..1756
                     /gene="mltG"
                     /locus_tag="PKI33_05225"
     CDS             590..1756
                     /gene="mltG"
                     /locus_tag="PKI33_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNM36439.1"
                     /translation="MNDTQPQAPVRRRKPRNSMRRYQLYFALGLIVLVAFICIFVFIY
                     YVPAQATLLYGPPAGHLSISDRIEYSTRLLSHGIELTTPLNVNGLEQSFRVEPGESVA
                     SIANRLEGLGIISSADAFFDYAVYTGIDLTIQSGDFNLSSAQSIVDIAQSLQKFNPSD
                     AVLSILPGWRIEEIAASLPTSGLSIAPETFLAAAQTPPQVLAFAAPTTMEGFFFPDTY
                     TLPRTTTVDQLLDVVARNFTTKLTTDIQNGFAAQNLSVYQGVILASIVQREAVKIEEA
                     PVIASVYLNRISIGMKLDADPTVQYALGYQFDNGSWWKSPLALTDLEVISPYNTYLNN
                     SLPPTPISSPGLEALMAVAYPQSTPYYYFQAACDGSGYHVFVVTYEEQIANGCQ"
     gene            1753..2187
                     /locus_tag="PKI33_05230"
     CDS             1753..2187
                     /locus_tag="PKI33_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36440.1"
                     /translation="MNIDDLKMRLEEEACSRANYSIGERDSDVFCLENQNGIWRIFYT
                     ERGIDQPPIFESPNESEACEYFFTFMTTKIRHDHIVGYFISREKADALVETLAQHNIQ
                     TYRNDIPYHGWHDPRFRVFVIGKDVFKAREILGELPVRDIRD"
     gene            complement(2184..3299)
                     /locus_tag="PKI33_05235"
     CDS             complement(2184..3299)
                     /locus_tag="PKI33_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36441.1"
                     /translation="MRRIFIFLSFLLVLSLACGGSPTPPPPLETSIFDSSETAYGFYP
                     SPPEVTLESVMQTFKAMGEHGDFVLIQQNVDWESFAKGVDGESQLRTDIINQVTLSRQ
                     NNLDTVFVLDALNGLNRREFFNLPSTWEANFGNPEVRTAFKNYALWVMRNFQPHYLGL
                     ASEINTYMDAHPDDAQNFISLYNEIYGLVKAEAPDTQIFVTFQWDDLNNMFAQPEEGN
                     AQRLSPKWEQIEAFEPNLDVWAISSYPYFVFPAGADIPSDYYAPLLSRTSKPVALAEG
                     GFSTVAFNELNNTPEDQVAYLNAIHEQLGPRMAFWVNTLLSDFNIDSYAKEMEKQGRD
                     PNDAQMLGNFAYIGLREFDGTPKPALEVWDGFRNSTP"
     gene            complement(3341..>3702)
                     /locus_tag="PKI33_05240"
     CDS             complement(3341..>3702)
                     /locus_tag="PKI33_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36442.1"
                     /translation="LLMRLSLILPIGCLLALTLFAIRSLQDWLTWWGVPLAATGFSSF
                     VIGLIGAPILGAVLESILSRKLPDYLPSFLLDFTSDFAAAMVRALLTPVAWQGLLISF
                     LGACMAGVGYYLKYKQP"
BASE COUNT          984 a          888 c          884 g          946 t
ORIGIN      
        1 attgtgcagg ttttagacct cattcgcgga cgtaacatcc aaaaggcggc tcagttggtg
       61 aaagatacat tggtattgca aaatgaaccg cagatcgaga tcacgccttc ttggtggaaa
      121 tggctgccat tgatcccctt taatatttca gtgaaagtac agtagacaat cgtatgagaa
      181 tccttgccgt tgaccacggt gaaaaacgaa tcggtcttgc tctcagcgat ccaaccgcca
      241 cgattgccag cccattgaaa gtgattcaac atgtctcacg tttaatcgat gcggctcagg
      301 tggcagagat tgccatgcaa aatgaagtag gtctaatcgt tgtcgggcag tcgtatgatg
      361 aagaaggcga ccccaaccca gccgggcgca gagccgggcg ctttgccgat gagcttcgta
      421 atcaaaccaa tattcccatc gaaatgtggg atgaatctca cagcactcag atcgcccgcg
      481 ccgctcgtat tgaacttggc gtttcacgca agaaacgtgc cgggcatcaa gatgagttcg
      541 ccgcagtggt catcttacaa tcttatttag aagcgaaacg taattccgaa tgaacgatac
      601 tcaaccacaa gccccagttc gaagacgaaa acctagaaac tccatgcgcc gctatcaact
      661 ctattttgct ttagggttga ttgtattggt tgcttttata tgtatctttg tgtttatcta
      721 ttatgtccct gcgcaagcca cacttttgta tggtccacct gccgggcatc tttccatttc
      781 agaccgtatc gaatattcaa ctcgcttact ttcgcacggt attgaactca caaccccttt
      841 gaatgtgaat gggctcgaac aatcattccg ggtagagccg ggcgagtccg tggcgtccat
      901 tgcgaatcgg cttgaggggt tgggcattat ctcaagtgcc gatgcgtttt ttgattacgc
      961 tgtttacacc ggcattgacc tcaccattca atctggcgat ttcaacctca gctcggcaca
     1021 atcgattgta gacattgctc agtcgctcca aaaattcaat cccagcgatg ccgtcctctc
     1081 gatcttgccc ggttggcgga ttgaagagat cgccgcctca ctcccgacct cgggcttgtc
     1141 cattgcaccc gaaacttttc tcgccgccgc acagactccg cctcaagtgc ttgcctttgc
     1201 cgctcccacc accatggaag gatttttctt cccagataca tatacccttc cacgcacaac
     1261 cacagtagac caattgctcg atgtggttgc gcgtaacttc accactaaat tgacaaccga
     1321 tattcaaaac ggttttgctg cgcagaatct cagcgtttat caaggggtga tccttgcctc
     1381 tattgttcaa cgagaagctg taaaaatcga agaagcgccg gtcattgctt ctgtctatct
     1441 caatcgcatt tccatcggta tgaaactaga tgcagatcct acagttcaat atgcattggg
     1501 atatcaattt gataacggct cttggtggaa gagtccgctg gcattgaccg acctggaagt
     1561 catctcgcca tataacacct atctaaacaa tagcttgccg cccacgccca tctcaagccc
     1621 cggtcttgag gcgttgatgg cggtggcata cccacagtct acgccgtatt attattttca
     1681 agcggcgtgc gatggctctg ggtatcacgt ctttgtagta acctatgaag agcagatcgc
     1741 gaatggatgt caatgaacat tgacgacctg aaaatgcgtt tggaagaaga agcctgtagc
     1801 cgcgccaatt attcgattgg tgagcgcgac tcagatgtct tttgccttga aaatcaaaat
     1861 ggaatctggc ggatttttta taccgaacgc ggtatagacc agcctccgat ctttgaaagc
     1921 ccaaacgaat cagaagcttg tgaatatttc ttcaccttca tgaccaccaa aattcggcat
     1981 gaccatattg tgggctattt catttcacgt gaaaaagccg atgccttggt agagacattg
     2041 gctcaacata atatccaaac ctaccgaaat gacattccct accatggctg gcatgacccg
     2101 cgtttccgag tatttgtgat tggcaaagat gtgttcaaag cccgcgagat tttaggcgaa
     2161 ttgcctgttc gtgatattcg agattaaggt gtggaatttc taaaaccatc ccaaacttcc
     2221 aatgcaggtt tgggtgtgcc atcaaattca cgtaagccaa tataagcgaa gtttccaagc
     2281 atttgagcat cgtttggatc gcgcccttgt ttttccattt ctttggcata agagtctatg
     2341 ttgaagtcac ttaacaaggt gttcacccaa aacgccatgc gtggacctaa ctgctcgtga
     2401 atagcattca ggtatgcaac ctggtcttca ggggtgttgt ttagttcgtt gaaagcaacc
     2461 gtggagaatc cgccttcggc aagggcaact ggtttcgagg tccgcgagag aagcggggca
     2521 tagtaatcag agggaatatc tgcaccggcg gggaagacga agtaagggta actggagatc
     2581 gcccatacgt ccaaattcgg ttcaaaagct tcgatctgtt cccactttgg cgaaaggcgt
     2641 tgcgcattgc cctcttcggg ttgtgcgaac atattgttca agtcatccca ttgaaatgtg
     2701 acgaagattt gcgtatcagg tgcctcagct ttgacgagcc catagatttc gttgtatagg
     2761 ctgatgaagt tctgcgcatc atcaggatga gcatccatat aggtgttgat ttcagaagcc
     2821 agcccaagat aatgcggttg aaaattacgc atcacccaca aggcatagtt cttaaaggcg
     2881 gtacgtacct ccgggttgcc aaagttggct tcccaagtgc taggcaagtt gaagaattcg
     2941 cgccgattta atccattcaa agcatcgagg acgaagacgg tatcgagatt gttttgcctt
     3001 gaaagggtca cttggttgat gatgtcggtt cgcagttgag actctccatc tacacctttt
     3061 gcgaaacttt cccaatctac attttgttgg atcaggacaa aatcgccatg ttctcccatt
     3121 gccttgaagg tttgcataac gctttcaagg gtcacttctg gtggagaagg ataaaagcca
     3181 taagccgttt cactggaatc aaagatagag gtttcaagtg ggggaggtgg agtaggagaa
     3241 ccaccgcaag ccaaagatag aacgagaaga aaggatagga aaataaaaat tcgccgcata
     3301 acttgtaata caagaagatg cggcgaaaag ttgcaagtga ttaaggctgt ttatatttta
     3361 ggtagtaccc aacccctgcc atacaagcgc caagaaaact gattaacaag ccttgccatg
     3421 caacgggcgt aagcaaggct cgcaccatgg ctgcggcaaa gtcgctagtg aaatccagta
     3481 aaaagctggg gagatagtct ggtaatttcc ttgaaaggat actttcaagc acagccccta
     3541 aaatgggagc accgatcagt ccaattacaa aactgctgaa gccagtggct gcaagcggaa
     3601 cgccccacca tgtcagccaa tcttgcaggg agcggatggc gaacagagtc aacgcgagta
     3661 ggcagccgat ggggaggatc aagctcaggc gcattaagag cc
//
LOCUS       DAOREF010000191         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15408_length_3700_cov_4.954458, whole genome shotgun sequence.
ACCESSION   DAOREF010000191 DAOREF010000000
VERSION     DAOREF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15408_length_3700_cov_4.954458"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKI33_05245"
     CDS             complement(<1..500)
                     /locus_tag="PKI33_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNM36443.1"
                     /translation="MSENFSRRDFLKLAGLGAATTAILTGCGPASRYVKREPYQQMPE
                     YNSVGQSTYYATTCRECAAGCGLVVRTYQGRALKTEGNANHPLNLGKTCARGQATLHG
                     LYNPDRVQGPSTGDWDSAIQTVADAFKTYKPSEIAFLMGMAPDHLFDLVSDLAKETGM
                     NAPVRYG"
     gene            complement(522..1079)
                     /locus_tag="PKI33_05250"
     CDS             complement(522..1079)
                     /locus_tag="PKI33_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNM36444.1"
                     /translation="MIKKFFDWLKQPLGLIAVGIVFLAVFSAAAYGLWTTQQAPAQPV
                     PGFPHKTHVAFGIQCLYCHPGAAKGPAAGIPSQAKCWACHNQIVKTRDSALLAPLKDA
                     VLSGTSLEWVPVAIVPDFVQFNHRAHIAAGKNCEECHGDMTQVTIAQNPQVMNMGWCL
                     NCHIQNAGEDQAKLIKLTDCGTCHY"
     gene            1465..1713
                     /locus_tag="PKI33_05255"
     CDS             1465..1713
                     /locus_tag="PKI33_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36445.1"
                     /translation="MKTKNIFNACIFLIAILTLTLVITNFNMPVAAAKDLPVTVLQQA
                     TPTPPVDSSEIGSTSGILVMGVVIVLIITVPLILQKRK"
     gene            1790..2497
                     /locus_tag="PKI33_05260"
     CDS             1790..2497
                     /locus_tag="PKI33_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36446.1"
                     /translation="MPEKILIVEDEIALQETLAYNLKKEGYTVETVGDGRSALEAARK
                     LKPDLIVLDIMLPEMDGFEVARTLRKEMTTGILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELIARVKAQLRRARLLREEMGKSVEETPHETLTFDNLIINLTRREVTLNGTPV
                     QLKPKEYELLLFLAEHRRQMLSREFILERVWGWDYIGDSRTVDVHIRWLRQKIEESSS
                     EPKRIVTVRGGGYRFEG"
     gene            2547..>3700
                     /locus_tag="PKI33_05265"
     CDS             2547..>3700
                     /locus_tag="PKI33_05265"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM36447.1"
                     /translation="MNQFAYATAIVFLIIAAWFAWKYYNLRKNVNQLAKSLHDPKIPL
                     PHLREIEELLSMIESRKATFSAELFSLQTENERLATVLEQLTDGVLIADADGRVQFAN
                     PAASKLFGTHNPTQQSVAQVVRSHQLIEAWKRCQQTRQLQIEAVEIATRKQYLQIIVI
                     PDRHEGGSLLLAQDLTQVRKLETVRRDFISNVSHELRTPLASLKALTETLQGGALADP
                     EAGPRFLDRIHTEVDALTQMTQELLDLSRIESGQVQLNFEMISPRKLIYSAADRMKAQ
                     VERAHLKLDVKCEDGLQNIKADKNRLEQVLVNLIHNAVKFNKPGGSITLSAEPTSGGV
                     RCAVRDTGVGVPMESLNRIFERFYRVDSSRTGSGTGLGLSISKHIIEAHGG"
BASE COUNT          989 a          921 c          893 g          897 t
ORIGIN      
        1 ccatagcgga caggagcatt catgccggtt tcttttgcaa ggtcagagac aaggtcgaag
       61 aggtggtctg gtgccatacc catgaggaaa gcaatctcac tgggtttgta ggttttgaag
      121 gcatcggcta cggtttggat agcagagtcc caatctccgg tggaaggacc ttgaacacgg
      181 tctgggttgt ataagccgtg aagagtggct tgcccgcgtg cacaggtctt accgagattg
      241 aggggatgat tggcattgcc ttcggtcttg agagcacgac cctgataggt gcgcacaacg
      301 agaccacatc ccgccgcgca ttcgcggcat gtagtggcgt aataggtact ctgcccgacc
      361 gaattgtatt caggcatctg ctgataaggt tcgcgcttaa cataacgcga agcgggacca
      421 caacctgtga ggatcgcagt ggtcgccgct cctaaaccag ccagttttag aaaatcacgc
      481 cgggaaaaat tttcgctcat ataggtttcc tttcacccgg tttaatagtg acacgtacca
      541 cagtctgtca gcttgatcag ttttgcctga tcttcgccag cattctggat gtgacaattc
      601 aaacaccagc ccatgttcat cacttgcgga ttttgggcaa tggtcacttg agtcatatcg
      661 ccgtgacatt cttcacagtt cttccctgcc gcaatatgcg cacgatggtt gaactgtaca
      721 aagtctggca cgatggcaac ggggacccac tcaagcgatg tgccgctcag gactgcgtcc
      781 ttaagtgggg caagcaaagc actatcacgg gtcttaacga tctgattgtg gcatgcccag
      841 cactttgctt gtgatggaat gcccgccgca ggacccttcg ccgcgcccgg atggcaatac
      901 aaacattgaa taccgaaggc aacatgggtc ttatgaggga accctggaac aggttgagca
      961 ggtgcttgtt gggtagtcca taacccataa gcagccgcgc taaacactgc caggaacacg
     1021 atccccaccg cgatcagacc aagaggctgt ttcagccaat cgaaaaactt tttgatcatg
     1081 gatgctcctg aaataaacta agcgtaacgc cgccattgtt gccaatggtg gcgtttgcaa
     1141 aattaccaac ttcttaatga caaacatgta gcgcttttgc tggtctctct ctttataaaa
     1201 agaacccacc caaattactt tacaagacag ccgcgctctg agggtatttt tcgtgcatct
     1261 gcctgcactg atgcacaact tgtaaattat acatcgaacg tgcaaaaagg tacaagttat
     1321 ccgatttgtg ttttaagccc aaacaggact tttttggtca aaatttctca acttaacgcg
     1381 ctttaatatt gatgtgattt tgatgttatg tttaaaaaga acactccgaa caaaagaatg
     1441 gtttcacctt ataatgaacc tacgatgaaa acaaaaaata ttttcaacgc gtgcattttt
     1501 ttgatagcca tcctaactct cacgttggtg atcacaaact ttaacatgcc tgtagctgcc
     1561 gccaaagatt tgccggtcac cgttttacag caagccaccc ctacccctcc cgttgacagt
     1621 tcagagatcg gctccacaag cggaatcctg gtcatgggag tggtcatcgt tctgatcatc
     1681 actgtgccgt tgatcctcca aaaaagaaaa taaaacggat tggtttgaag ttcaagccat
     1741 taacctcacc ttaacagatc agggattcct ttatggtata gtgccactca tgcctgaaaa
     1801 gatattgatc gttgaagacg agatcgctct tcaagaaacc cttgcctata acctaaaaaa
     1861 agagggctat accgttgaaa ccgtaggcga tggtcgctct gcgctcgaag ctgcccgtaa
     1921 actaaaacca gacttgatcg tgctcgacat tatgttgcca gaaatggatg gcttcgaagt
     1981 ggcgcgcaca ttacgcaaag aaatgacaac tggcattctg atgctcaccg cccgcgatga
     2041 cgagatcgac cgtgttgttg ggctggaagt gggcgccgac gattacctaa ccaagccttt
     2101 ctcgatgcgt gaattgatag cacgcgtcaa agcgcaatta cgccgtgccc gcctattaag
     2161 agaagagatg gggaaatctg tagaagaaac gccacacgaa accctcacct ttgataacct
     2221 catcatcaac ctcacccgcc gcgaagtgac attaaacggc acccccgtac aactgaagcc
     2281 aaaagaatat gagcttttgc tctttcttgc cgaacaccgc cggcaaatgc tgtcacgtga
     2341 atttattctg gaacgcgttt ggggttggga ctatattggc gatagccgca ctgtcgatgt
     2401 gcatatacgt tggttgagac aaaagatcga agaaagttca agtgagccga aacgtattgt
     2461 taccgtccgt ggcggcgggt atcggtttga aggatgaagt atgaggagca acctacggct
     2521 ctctcattac gtattgtgac ccacgtatga atcaatttgc ttatgcaaca gctattgtat
     2581 ttctcatcat tgcggcgtgg ttcgcatgga agtattacaa cctgagaaaa aacgtcaatc
     2641 agttggcgaa atctctgcat gaccctaaaa ttccgctccc ccatttaaga gaaattgaag
     2701 aactgcttag catgattgag tcgcgcaagg caactttctc tgctgagctt ttctctctgc
     2761 aaacagaaaa tgagcgcctt gctacagtcc tcgaacagct taccgatggc gtgctcattg
     2821 ccgatgccga tggccgggtg caatttgcaa accctgcggc aagcaaactc tttggcactc
     2881 acaaccccac acaacaaagc gtggcacagg tggtgcgcag tcatcaatta attgaagcgt
     2941 ggaagcgctg ccagcaaacc cgtcaacttc aaattgaggc agtagaaatc gccacacgca
     3001 aacagtattt gcagatcatc gtcattccag accgccacga aggtgggagt ttattgctcg
     3061 cgcaagacct cactcaggtg cgcaaactcg aaaccgtacg gcgggacttt atctcgaacg
     3121 tctcccatga attgcggacg ccgcttgctt cgctaaaggc gctcaccgag acattacaag
     3181 gcggcgctct cgcggacccg gaagctggtc cgcgtttctt agaccgaatc cacactgaag
     3241 ttgacgcgct gactcagatg acccaagaac tgctcgacct ttctcgcatt gagtctggac
     3301 aagtgcagct caactttgaa atgatctcgc cgcgtaaact catctactct gccgcagacc
     3361 gaatgaaagc acaagtggaa cgtgcgcatt tgaaattaga tgtaaagtgt gaagatgggt
     3421 tgcaaaatat caaagccgat aaaaatcggt tggaacaggt gttggtcaac ctgattcaca
     3481 atgcggtcaa attcaacaag cctgggggga gtatcactct gagtgcagag ccaacttcgg
     3541 gcggggtacg atgcgcagtg cgggatacag gtgtaggagt ccctatggag agtctcaatc
     3601 gcatctttga acgcttctat cgcgttgatt catcccgcac cggttctggc acaggattag
     3661 ggctttcgat ttcaaagcat attatcgaag cgcacggcgg
//
LOCUS       DAOREF010000192         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15425_length_3698_cov_4.932199, whole genome shotgun sequence.
ACCESSION   DAOREF010000192 DAOREF010000000
VERSION     DAOREF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15425_length_3698_cov_4.932199"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PKI33_05270"
     CDS             <1..1527
                     /locus_tag="PKI33_05270"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNM36448.1"
                     /translation="ESRLRYLLPSEMYAEMWGNPSMEIMIRVHNHLRTLQRIIHDYTS
                     RQIDVSQLKPGQVKTEWQYGTMMFTDLAGFTKLMEANASKGRDGAENLLKELTKYFST
                     MISIISKSGGELVEFTGDAMLVVFAKKKEKNDDAQRAIRAGLRMQRAMKDFAEIQTPS
                     GIVNLQMRIGIHSGRFLTAEIGTPRRMEHVLLGKDVQKAKLTESKGRNERVNVSVTAY
                     ELAKDSFRFDEGEPDYHLVVDDLTADSLGDYEITPMGRRMASSVLFDLKDKKEVLSNI
                     TELLNSIEPLASYIPDPVLSMLVESAADRKIRPDFPTPTIMFVNFIGLPEAADRALPG
                     EERKLALSFSKAFSLINAAVEARGGVLKKVTYHLSGSDIVIYFGVPNAHTNNEMRAAS
                     AAVAIREIVESINLKAPTIGGIKPEVYCQIGINMGPAFVAEVGDPRGRREFNVLGDTV
                     NTAARLMNRAQKNQIVLSETVKQAIESKYECTPLGEIELKGKSHPMSLYELTKKKPKE
                     "
     gene            complement(1570..1929)
                     /locus_tag="PKI33_05275"
     CDS             complement(1570..1929)
                     /locus_tag="PKI33_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36449.1"
                     /translation="MPGLFDRLDKEIKDNQKKDGISPLDIAKLPPAMRKIMRVMLREL
                     QASYPRMCEVVDAMPEQDRLTRTELDESLATLTEQLWLIRIGEKEKAIYKVNLRRKTG
                     STLMAGIWSSLDTKLKR"
     gene            complement(1941..2153)
                     /locus_tag="PKI33_05280"
     CDS             complement(1941..2153)
                     /locus_tag="PKI33_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36450.1"
                     /translation="MKCWHCEQEARASCSFCGRFVCKDHASTMSTFITMFVGANNTPK
                     GLAVANVIWCGECEPQPEPISMPELY"
     gene            complement(2163..2951)
                     /locus_tag="PKI33_05285"
     CDS             complement(2163..2951)
                     /locus_tag="PKI33_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNM36451.1"
                     /translation="MSMGKIVSIHSFRGGTGKSNTTANLAAQVALAGKRVGVVDTDIQ
                     SPGIHVLFGLDDEKMGKTLNDFLHGRATIREVGFSVGANTGTEDGRAKLAGKDIWLFP
                     SSIKGGEISQIIKEGIDFNVLNEGLQTAISEFDLDYLFVDTHPGLNEETLLSIATSDI
                     LIIILRPDNQDLQGTSVTVDVARSLDVPNLLLMVNKALPKHLNHPEFNKEIEGIFDAP
                     VTGVLPLSFDMAENASKDLFSLRYPDHEWSKALRNVVPFILNAK"
     gene            complement(2967..>3698)
                     /locus_tag="PKI33_05290"
     CDS             complement(2967..>3698)
                     /locus_tag="PKI33_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNM36452.1"
                     /translation="GDILQKLSKTEFFKEFSEEALGAVAGKATLRHFAANEALMRKGD
                     PADSFFVILDGHVKIVTTGARGEEIIINKSNPGETIGELALVDERPRSAGVIALDDVE
                     ALELTKDALFSLLDQRLDVSLGILRAFSNRLRFSTTYIEKVIDWSQKTAEGDYSFLDA
                     TQPIGNSVRNDDDKAAQLLSTFYSMVHRVKAREEGLKQQVEKLTFQIDQERRKREFEE
                     ITGTEFYAKLKEQAQTIRKKRSQDS"
BASE COUNT         1007 a          940 c          830 g          921 t
ORIGIN      
        1 gaaagccgct tgcgttatct gctcccctct gaaatgtacg ccgaaatgtg gggcaacccc
       61 tcgatggaga tcatgattcg ggttcacaat catctgcgaa ccctgcaacg tatcatccat
      121 gattacacat caaggcagat cgacgtctcc cagttaaaac cgggacaggt gaaaaccgaa
      181 tggcagtacg gaaccatgat gttcaccgac ctagcaggtt tcaccaaact gatggaagcc
      241 aacgcatcca agggacgtga cggtgcggaa aaccttctca aagaattaac aaaatatttt
      301 tcaaccatga tctcgatcat cagtaaatcg ggtggtgaac tggtagagtt cactggcgat
      361 gcaatgctgg tggttttcgc aaagaaaaag gaaaaaaatg atgatgccca gcgcgccatc
      421 cgcgccgggt tgcgtatgca gcgggcaatg aaagatttcg cagagatcca gaccccttcg
      481 ggaatcgtca acctgcaaat gcgcatcggc atccacagcg gcaggtttct aacggcagaa
      541 attggtacgc cgcgtcgcat ggagcatgtg ctgctcggta aagatgtgca aaaagccaaa
      601 ttgaccgaaa gcaagggacg caacgagcgc gtgaacgtat ctgtcacagc ctatgaactg
      661 gcaaaggaca gtttccgctt tgatgaaggc gaacccgact atcacctggt tgtggacgac
      721 ctgacagcgg atagccttgg cgattacgaa atcacaccca tgggacggcg catggcaagt
      781 agtgttctat ttgacttaaa agacaaaaag gaagtcttga gcaacatcac tgaactgctc
      841 aactccattg aacctctcgc cagttatatt cctgaccctg tcctttcgat gcttgtcgaa
      901 agtgcggcag accgcaagat ccgcccagac ttccccaccc ctaccattat gttcgttaac
      961 tttatcggtt tgccagaagc agcagaccgt gccctgcctg gcgaagagcg caaattagca
     1021 ctcagtttct cgaaagcttt ctcgcttatc aatgcagcag tagaagcgcg cggcggggtg
     1081 ttgaaaaaag tgacctacca cctttcaggt tcagatatcg tcatatactt tggcgttccg
     1141 aacgctcaca ccaacaatga aatgcgtgct gcatctgcag ctgttgccat ccgcgaaatt
     1201 gtggaatcga tcaacctcaa ggcacccacg atcggaggca tcaagcctga agtgtattgc
     1261 cagatcggca tcaatatggg accggcattc gtggcagaag ttggtgaccc acgcggcaga
     1321 cgtgagttca atgtgctggg ggatacggtc aatacagcgg ctcgcttaat gaaccgtgcc
     1381 caaaagaatc agatcgtcct ctcagagaca gtcaaacaag cgattgagtc aaagtatgaa
     1441 tgcacgccct tgggtgagat cgagctaaag ggcaaaagcc accccatgag tttgtatgag
     1501 ctaaccaaga aaaaaccaaa agaataaaaa aacgccgcga tcgcggcgtt tttatttaag
     1561 tagaattggt tatcgcttaa gttttgtatc gagcgaagac catatccctg ccattaaggt
     1621 gctgcccgtt ttacggcgca aattaacttt atagatcgcc ttttcctttt caccgatccg
     1681 aatcagccat agttgttccg tgagtgttgc aagagactca tctaattcgg tgcgcgtgag
     1741 gcggtcttgc tcaggcatgg catcgaccac ttcgcacatt cgcggatagc ttgcttgaag
     1801 ctcgcgcaac atcacacgca tgatcttacg catggcaggc ggaagcttgg caatatctaa
     1861 tggagagatg ccatctttct tttggttgtc ttttatttct ttatcgaggc gatcaaaaag
     1921 accaggcatg gttccctcgc ttaatacaac tcaggcatac tgatcggttc gggttgtggc
     1981 tcacattcac cacaccagat cacattagcc actgccaacc ccttgggggt attattcgca
     2041 cccacgaaca tcgtaataaa cgtggacatg gtcgaagcat ggtctttaca aacgaaacgt
     2101 ccacaaaatg agcaggatgc ccgggcttct tgttcacaat gccagcattt cataagaggc
     2161 tcctattttg cattcaggat gaacggcaca acgttccgca aagccttcga ccattcatgg
     2221 tctggatatc gcagagaaaa taggtctttg ctggcatttt ctgccatatc gaaagaaagg
     2281 ggcaaaacgc ccgttacagg cgcatcaaaa atcccttcga tttctttatt aaactcaggg
     2341 tgattcaagt gtttcgggag tgctttattg accatcaaca aaagattcgg cacatccaaa
     2401 ctgcgagcaa catcgacggt aacggatgtt ccttgcaggt cctgattatc tggtcgcaag
     2461 atgatgataa gaatatcaga tgtagcaata gaaagtaaag tttcttcgtt taatccgggg
     2521 tgtgtatcca caaaaaggta atcaaggtca aattcgctga tcgcggtttg caaaccttcg
     2581 ttcaacacat tgaaatcgat accttccttg atgatctggc tgatttcgcc acccttaatg
     2641 ctggacggaa acaaccaaat atctttccca gctaatttgg cacgtccatc ttctgtgccg
     2701 gtgtttgctc ccactgaaaa accgacctcc cgaatcgtgg cacgtccatg caaaaagtca
     2761 ttcagggtct tccccatttt ttcatcatca agcccaaaaa gcacatgaat ccctggagac
     2821 tgaatgtctg tatccacaac gccaactcgt tttcccgcca acgctacttg cgctgctaaa
     2881 ttagcagtag tatttgattt acccgtccca ccacgaaagg aatggatcga aacgatcttg
     2941 cccatgctca tgaaaagcct ccgttgttat gaatcctgcg aacgtttctt acgaatggtc
     3001 tgcgcctgtt cctttaattt agcatagaac tcggtgcctg tgatctcttc aaattcacgc
     3061 ttacgccgtt cctgatcgat ctggaatgtc aatttttcta cctgttgctt caacccttct
     3121 tcacgcgcct tcactctatg caccatggaa taaaaagttg aaagcaattg cgcagccttg
     3181 tcatcatcat tcctgacgct atttccaatc ggctgggtgg catctaaaaa ggagtaatct
     3241 ccttctgctg tcttctgcga ccaatcgatt actttttcaa tataggtcgt tgaaaatctt
     3301 aatcggtttg aaaaagctcg cagaatacca agagacacat ccaaacgttg gtcaagaagc
     3361 gaaaacaaag cgtctttggt gagttccaag gcttccacat catccaacgc tatcacaccc
     3421 gcagaacgag ggcgttcatc caccaatgcc aattcgccaa tcgtctcgcc agggttactc
     3481 ttgttgataa taatttcttc cccacgcgcc cccgtagtaa cgattttgac atgtccatcc
     3541 agaatcacaa aaaaggaatc agcggggtcg cctttgcgca tcaaggcttc attggcggca
     3601 aaatggcgca aagttgcctt tccagcgaca gcccctaaag cttcttctga aaactctttg
     3661 aaaaattcgg tcttactcag tttttgcagg atgtcgcc
//
LOCUS       DAOREF010000193         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15570_length_3680_cov_3.566345, whole genome shotgun sequence.
ACCESSION   DAOREF010000193 DAOREF010000000
VERSION     DAOREF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15570_length_3680_cov_3.566345"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PKI33_05295"
     CDS             <1..1510
                     /locus_tag="PKI33_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNM36453.1"
                     /translation="MDAIAKSMQGAYYWGTGGWGDAPKFPQPMVLEFLLHHAVTTKQN
                     EYLKLIEHCLKVMARGGMYDVIGGGFSRYSTDDHWRVPHFEKMLYDNAQLVRVYLHAW
                     QVTHDPFYKRIVEETLEFIAKEMTHPQGGFYSSLDADSEGEEGKFYVWSLDEIREVLK
                     DESEFFEAAYGVSAKGNWEGNTVLQRALDDASLAARFKLDPEAVPAKLAESHSKLYAA
                     RSKRIRPGTDDKVLTSWNGLMLAAFAEAARVLAGVSPKAITYSNLATRSAEFLLSNLR
                     VDGRLRRSWRDGKTTNEVFLEDYASLILGLLEMYQTDFNTKWFTAAKELADEMVDKFQ
                     DPHGGFFDTPIDGENLLFRPKDVQDNATPSGNSLACEALLKLAAYTDNGNYRDIAEKS
                     FGLVHKFVLQYPLGFGRWLTAAQLATGTLKQVAAVGEAGDPNFQSLLKVIWSEYRPNV
                     VVAASSSLEKNTPALLHDRPLMHGKATVYVCEGFVCQQPTTEADVLAEQLKN"
     gene            complement(1546..1725)
                     /locus_tag="PKI33_05300"
     CDS             complement(1546..1725)
                     /locus_tag="PKI33_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNM36454.1"
                     /translation="MGLPRGSEWIFILIIVVLVFGPGRIGKVAGEIGKSIKSFRDGLS
                     GEEEAEKKSDTTEQK"
     gene            complement(1784..2887)
                     /locus_tag="PKI33_05305"
     CDS             complement(1784..2887)
                     /locus_tag="PKI33_05305"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNM36455.1"
                     /translation="MPYAHITGWGVSVPEPVLTNDDLAKMVETNDEWIRERTGIRERH
                     IAREDQSTATLAVDAAIKALQVANLAPTELDLIIVTSSSPEYIFPATACLVQDQIGAT
                     KAGAFDLLAACSGFIYATNMAAQSIRSGSIKNALVIGSETLSRFVNWKDRSTCILFGD
                     GAGAFVIQASDQPGGVLSAVMHSDGSGADSLTLLGGGARFPANETTVAEGKHYVQMDG
                     KAVFRFATRVMGTATKEALELANVKLEEVDYIIPHQANYRIIETAAKYLKLPLDKFII
                     NVDRYGNTSTASIPIAAVEAIEKGKLKSGNKVVFVGFGAGLTWGALVAQWSGPIPAKK
                     QVHPEQYRLFARLRSWLLRALRFLEGLLSRREL"
     gene            complement(2897..>3680)
                     /locus_tag="PKI33_05310"
     CDS             complement(2897..>3680)
                     /locus_tag="PKI33_05310"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNM36456.1"
                     /translation="RGPNMSLATACASGTNGVGEAAEMIRRGAADVMVAGASEAAIHS
                     LTMAGMNVMTALSTRNDDPQRASRPFDKDRDGFLMGEGSAVLILESLEHAQARGANIL
                     CEFTGYGTSDDAHHISAPAENGAGAAISMSLALKDAGFGTTDIQYINAHGTSTQLNDK
                     SETAAIKTVFGEQAYKLAVSSTKSMTGHLLGASGALEAMISALTIMNQTLPPTINYET
                     PDPNCDLDYVPNQPRKTNVTNVMSNSFGFGGHNATLVLSRYQ"
BASE COUNT          989 a          910 c          855 g          926 t
ORIGIN      
        1 tatggatgcc attgccaaat ccatgcaagg cgcgtattac tggggcacag gcggttgggg
       61 cgatgcgcca aaatttccgc aaccaatggt tcttgaattc ttgcttcatc atgccgtcac
      121 tacaaagcaa aatgaatact taaaactgat tgagcactgt ctcaaagtaa tggcacgcgg
      181 cgggatgtat gatgtcatcg gcggcggctt ctcgcgctat agtacagatg accattggcg
      241 tgtgccccat tttgaaaaaa tgctttacga caacgcgcaa cttgtccgtg tatatttgca
      301 tgcctggcaa gtgacacacg acccattcta caaacgcatt gtcgaagaaa ctcttgaatt
      361 catcgcgaaa gaaatgacgc acccacaagg cggtttttat tcttcgctcg atgctgactc
      421 ggaaggcgaa gaaggcaagt tctatgtatg gagcttagat gaaattcgtg aggtattaaa
      481 agatgagtct gaattttttg aagcggcata tggagtgagc gccaaaggca attgggaagg
      541 aaataccgtc cttcagcgtg cattagacga tgcctccctt gccgcccgct tcaaactgga
      601 cccggaagct gttcccgcca aattagccga atctcactcc aagctttatg ctgcccgctc
      661 caaacgcatt cgcccgggaa cagatgataa agtcctcacc tcatggaatg gattgatgct
      721 cgctgccttc gccgaagccg cccgagtcct tgcaggcgtt agtccaaaag cgatcactta
      781 ctcaaatctg gctacgcgca gcgcagagtt tttgttatcc aacttgcgag ttgatggcag
      841 attacgccga tcgtggcgag atggcaaaac caccaatgaa gttttccttg aagactatgc
      901 atcattgatc cttgggctat tggaaatgta ccagaccgat ttcaacacca aatggttcac
      961 tgctgccaaa gaactcgcag acgaaatggt cgataaattc caagacccgc atggtggctt
     1021 ctttgatacc cccattgatg gtgaaaacct cctgtttcgc ccaaaagatg tacaagataa
     1081 tgcaacccct tcagggaatt ccttagcttg tgaagcctta ttaaagttag ccgcatatac
     1141 tgacaatggt aactatcgtg atattgcaga aaaatcattt ggcttggtgc ataaatttgt
     1201 cttgcaatat ccgcttgggt ttgggcgctg gcttacagcg gcgcagcttg ctacaggaac
     1261 gctgaaacag gtagcggcgg tgggagaagc gggagaccca aacttccaga gtttgctcaa
     1321 agttatttgg tctgaatata gaccgaacgt ggtggtggct gcatcgtctt ctttagaaaa
     1381 aaacaccccc gcattgctcc atgaccgtcc actgatgcat ggtaaagcca ctgtgtatgt
     1441 gtgtgaaggc tttgtttgcc aacagccaac aacagaagct gatgtgttgg ctgagcagct
     1501 aaaaaattaa acaaaaaaca gaccgtgatt acggtctgtt tttaattact tctgttctgt
     1561 tgtatcggat ttcttctctg cttcttcttc gccgctcaaa ccatcacgga aagatttgat
     1621 gctcttgccg atctcaccag ctactttgcc aatgcgaccc ggaccaaaaa caagaacaac
     1681 gataatcaga atgaaaatcc actctgaacc acgtggtaaa cccatagtac gctccttgat
     1741 tgaatttcat atcaatcgta ccatagttca aatgaaaact tgattagagt tcgcggcgag
     1801 agagcaaacc ttcaaggaaa cgcagggcac gtaacagcca tgaacgaagg cgggcaaaca
     1861 aacgatactg ctcggggtga acttgtttct ttgcagggat gggaccgctc cattgtgcaa
     1921 cgagtgcgcc ccaggttaaa cccgcaccaa agccaacaaa gaccactttg ttaccgcttt
     1981 ttagtttgcc tttttcaatc gcttctactg cagcaatggg aatagatgcc gtggaggtgt
     2041 taccgtaacg atcgacattg ataataaatt tatctaaggg cagctttagg tattttgccg
     2101 ctgtttcaat aatgcggtag tttgcctgat gcggaataat atagtccact tcttcaagtt
     2161 tcacgttggc aagttcgaga gcttctttgg tcgcagtacc catcacgcgt gtagcaaatc
     2221 gaaaaacagc tttgccatcc atttgcacat aatgcttgcc ctctgcaact gtggtttcat
     2281 tagctgggaa gcgtgctcca ccgcccaaaa gcgtcaagga gtctgcgcca gagccgtctg
     2341 aatgcatgac cgccgataag accccaccgg gctggtcact tgcttggata acgaaggctc
     2401 cagcgccatc gccaaaaaga atacaagtgc tgcggtcttt ccagttcaca aaacgagaga
     2461 gggtctcaga gccaatgact aaggcatttt tgattgaacc acttcgaatc gactgtgccg
     2521 ccatattggt ggcgtaaata aaacctgaac acgctgcaag taaatcgaat gctccagctt
     2581 tagtagcacc gatctgatcc tgcaccagac atgcagttgc tgggaaaata tattctggcg
     2641 aagaagatgt aacgatgatc aaatccaatt cagttggcgc taaattagcc acttgtaaag
     2701 ctttgatcgc cgcatcaact gccagcgtcg ctgtggattg gtcttctcgg gcgatgtgac
     2761 gttcacgaat cccagtacgc tcgcgaatcc attcatcatt ggtctcgacc atttttgcaa
     2821 ggtcgtcatt cgtcaatacc ggttcaggaa cacttacacc ccaacccgta atatgcgcgt
     2881 atggcatgct ttctccttat tgatagcggc taagtaccaa agtcgcattg tgtccaccaa
     2941 aaccgaacga attagacata acattggtca cattcgtctt gcgcggttga ttggggacgt
     3001 aatcgagatc acagttcgga tcgggagttt cataattgat cgtaggcggc aaagtctggt
     3061 tcatgatggt caaggcagaa atcatggctt ccaaagcgcc agaagccccc aaaagatgcc
     3121 cggtcatcga cttggtagaa gacaccgcaa gtttataggc ttgttcacca aacacagttt
     3181 tgatcgccgc ggtttcgctc ttgtcattca actgcgtgga tgtaccgtga gcattgatat
     3241 attgaatatc agttgttcca aagccagcat ctttcaatgc caagctcatg gaaatagcag
     3301 cacctgcccc attttctgcc ggagcagaaa tatgatgcgc atcatctgaa gtgccgtagc
     3361 cagtaaattc acaaaggata ttcgcgccgc gagcctgggc atgttccaaa gactctaaaa
     3421 tcaataccgc cgatccttct cccatcaaga acccatctcg gtctttatcg aacggacgtg
     3481 aagcacgctg cggatcatca ttgcgggttg agagagcagt catcacattc atgcctgcca
     3541 tcgtcaagga atgaatggca gcttcagaag ccccagccac cattacgtct gcggcaccac
     3601 ggcgaatcat ttctgctgct tctcctacac cattcgtgcc agaagcacag gcagttgcca
     3661 aagacatgtt tggtccgcgc
//
LOCUS       DAOREF010000194         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15615_length_3672_cov_4.642798, whole genome shotgun sequence.
ACCESSION   DAOREF010000194 DAOREF010000000
VERSION     DAOREF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15615_length_3672_cov_4.642798"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..350)
                     /locus_tag="PKI33_05315"
     CDS             complement(33..350)
                     /locus_tag="PKI33_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36457.1"
                     /translation="MTQKSIFRWLSVLWLLTSQVAGAALVFAPLVVLLSFQEAAAAAG
                     EAGMFNLILGLGYVLPVVFVGLGIAAWVMLARSKNAVANWLGLATLAPGTIMLLMINM
                     IAP"
     gene            complement(385..2040)
                     /locus_tag="PKI33_05320"
     CDS             complement(385..2040)
                     /locus_tag="PKI33_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNM36458.1"
                     /translation="MPRRSLARLYRDEFAGYSLATFRNDLVAGLNVALVALPLALAFG
                     AASGATAAAGLVTSIVGGIIIGLLAGAPYQMTGAKAVISVVLIVLVQQYGLEGMWVAL
                     FMAGLLTLGIGLFRLGRFIAFIPSPVIAGFTAGISAIIIIRQFDPLLNIHTPTTITAA
                     QKLIGYFTTPNLSPDLHSLLMGLFVMGIMILYPTSWNKRIPGSLVALVIATASNSALG
                     WSIPTIHNIPRTLFLQPNFSIAEIPWQNISDFIAPALTLTALGAVETLLTGSIGAELT
                     GKRLQVNQHLIAQGIGNMILPFFGAIPTTSGIIRTKVAIQSGGQTRLTGIIHSLILLI
                     VMFAFADYLAMIPLSALASVLMVTAIRSVDLSSVKFTFGNFFKTDMIAFTVAFVATVV
                     LSLTNAILFGTFLMGAVFLNKSASIDINIQEVDVERLKQRGIETAGKCKHVRVAFLTG
                     PLFFAATSQFSESFADLGDTHALILSMRGVPLIDSAGIEAIHKLHVRLQKNGGTLMFA
                     GIHDNAKQMMERGGLVKTIGEENFFWSSDQAIVEAERRGCPFC"
     gene            complement(2202..2984)
                     /locus_tag="PKI33_05325"
     CDS             complement(2202..2984)
                     /locus_tag="PKI33_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36459.1"
                     /translation="MRTKAIYPILVLLCFSFACQALFPATPPPRDGVVIDNCSEFVKS
                     IRSMQTNDTPQELIETGIKQGGEFDVNEYFNVTPNLSMQEGYALDYVIIPDSLGSFPL
                     LAARPVDQPPYKSSSEVSVDSELTNYWKYMDVNDNEQGYFEYASFLLLANQFYLVWHA
                     NYNDLGIVCDRATVDNIVANVNDGGFGVEFDREQMKQIKALQNIEPLVKLTDTTAIVE
                     LVVFSNWGGFYRMTYTISRSSPHEIMDRQDENIIPYDCGIMF"
     gene            complement(3049..3411)
                     /locus_tag="PKI33_05330"
     CDS             complement(3049..3411)
                     /locus_tag="PKI33_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36460.1"
                     /translation="MSHTCEAIVVHCMDYRLQSYINQWLETNLGKGSYDRAVMAGGVY
                     DVYDVIRQVDISARLHGITKVVLINHEDCGMYGPGGTEERHEEDLDKAEDKIRRLFPH
                     LEVDSYYLNLDGTFERKS"
     gene            complement(3428..>3672)
                     /locus_tag="PKI33_05335"
     CDS             complement(3428..>3672)
                     /locus_tag="PKI33_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HNM36461.1"
                     /translation="EGRSVGLLLANEDMASFESACIPYPLGASNDLEALARNLYAGMR
                     ALDDQGVKLILTRDFGKQGLGLAIHDRLLRAASQII"
BASE COUNT         1038 a          857 c          868 g          909 t
ORIGIN      
        1 aaaaaccttt gcataaaagt tgaaccatat ttttatggcg caatcatatt tatcataagc
       61 aacatgattg tcccaggagc gagagtggca agccctaacc agtttgcaac tgcgtttttg
      121 ctgcgtgcta acatgaccca agcggcaatg cctagcccta caaacactac aggcagaaca
      181 tatcccaacc ccaatattaa attaaacatg ccagcttcgc ccgctgcagc ggcagcctct
      241 tgaaagctta acaatacaac gagcggagca aaaacaagtg cggctcctgc aacttgagaa
      301 gtcaatagcc ataaaacgct taaccaacgg aagatcgatt tttgagtcat gggatatctc
      361 cttgaaagta tatttcatta tatatcaaca aaagggacaa ccgcgcctct ctgcttcgac
      421 gatcgcttgg tcgctggacc agaagaagtt ttcttcgccg atggttttta caagaccacc
      481 gcgttccatc atttgtttgg cgttgtcatg aatgcctgcg aacataaggg tgccgccgtt
      541 cttctgcaag cggacatgca acttgtggat agcttcaatg cccgccgagt cgatgagagg
      601 caccccgcgc atggacagaa tcaaagcatg cgtgtcacct aagtctgcaa aggattcact
      661 gaattgtgag gttgccgcaa aaaatagcgg accagtcaga aaggcgacac ggacgtgctt
      721 acattttccg gcggtttcaa tgcctctttg tttgaggcgt tctacatcca cttcttgaat
      781 attgatgtca atgctcgcgc ttttgttgag gaagacagct cccattagaa aggttccaaa
      841 taggatggcg ttcgttaggc tcagaacaac ggtggcgaca aaggcaactg tgaaagcaat
      901 catgtcagtt ttgaagaaat tgccaaacgt aaatttgacg gagcttaagt ctactgagcg
      961 gatggcagtg accatcaaga cactagccag cgcagagagc gggatcatgg caagataatc
     1021 ggcaaaagcg aacatgacaa taagcagtat taaagagtgg ataatgcctg tcaggcgcgt
     1081 ctgcccgccg gattgaatgg caactttggt gcgaatgatt ccagatgtgg tgggaatggc
     1141 accaaagaat ggcaggatca tattcccaat accttgggca atcagatgtt gattgacttg
     1201 cagtcgtttg cctgtcagtt cggcgccaat ggatccggta aggagggttt caacagcacc
     1261 aagggcagtg agagttaaag ccggcgctat gaaatctgaa atgttttgcc atggaatttc
     1321 tgcgatgctg aaatttggtt gcaggaataa ggttcggggg atgttgtgga tggtggggat
     1381 ggaccagccc agagcggagt tggaggcggt ggcaatcact aatgcgacca gcgaaccggg
     1441 aatccgtttg ttccagctgg tagggtagag gatcatgatt cccatcacaa atagacccat
     1501 cagaaggcta tgaaggtctg gggagagatt gggagtggtg aaatatccga tcagtttttg
     1561 tgcggctgtg attgtggtag gggtatggat gttgagcagc gggtcgaatt gacggataat
     1621 aatgatggcg gaaatgcctg cagtaaaacc agcaataaca ggggatggga taaaggcgat
     1681 gaagcgcccg agcctaaata gaccgatgcc tagtgtgagc aaacctgcca tgaagagcgc
     1741 tacccacatt ccttcaagac catattgttg aacgagtacg atcaacacca cactgataac
     1801 ggcttttgca ccggtcattt gatagggcgc acctgccaga agtccaatga tgatgccgcc
     1861 aacaatggat gtcaccaacc cagccgcggc ggttgcaccc gaggcagcac caaaggcaag
     1921 cgcaagcggc aaagcgacca gcgcaacatt tagacctgca acgaggtcat tgcgaaatgt
     1981 ggcgagcgaa tagcccgcaa actcgtccct gtaaagacgg gcaagggaac gtcttggcat
     2041 atttaattct ccgtaaatag tagtacacca aaggctcggt gctcccccaa ggcgtccttt
     2101 ggtttatgtc gggcgatatt ttatcactgc tgattttatt ttgtatacgg atttaataga
     2161 ctacacggta ttcgtaatga atccgtgtag tctattttgt tctagaacat gattccacag
     2221 tcatacggaa taatattctc atcttgccta tccataatct catgcggaga cgatctgcta
     2281 atcgtatagg tcatgcgata aaagccgccc caattgctaa aaacaaccaa ttctacgata
     2341 gcagttgtgt ccgttagctt gacgagtggt tcgatatttt gcagagcctt gatctgtttc
     2401 atttgttctc tatcgaactc cacgccgaac cctccgtcgt tgacgtttgc aacaatatta
     2461 tcaacggttg cacgatcgca gactataccc aagtcgttgt aattggcatg ccaaactagg
     2521 tagaattgat tggcaagaag caggaaggaa gcgtattcaa aatatccctg ctcgttatcg
     2581 ttgacgtcca tatacttcca ataatttgtg agttcagaat ccacggagac ttcgctcgat
     2641 gatttatatg gtggttgatc taccggacga gcagccaaaa gcggaaagga acctaatgaa
     2701 tcggggataa tgacataatc cagcgcatat ccctcttgca tagaaaggtt tggtgtgacg
     2761 ttgaagtatt cattcacatc aaattcaccc ccttgtttga tgcccgtttc gatcaactct
     2821 tgaggcgtat cgtttgtctg catactgcga atcgacttaa cgaactctga acagttgtcg
     2881 atcacaaccc catcacgggg tggcggcgta gcaggaaaga gcgcctgaca cgcaaaagaa
     2941 aagcacaaca aaacaagaat tggataaatt gcctttgttc tcatcatatg ctccaaataa
     3001 aagggttgag aaaaagacga aagccgttct gaaaatgttc cttattggct aagacttacg
     3061 ctcgaaggtt ccatctaagt ttaggtaata tgaatctact tcaaggtgtg ggaataacct
     3121 gcgaatttta tcttcggctt tatcaaggtc ttcttcatgg cgttcttccg tcccaccagg
     3181 accatacata ccgcaatctt catgattgat cagcaccacc tttgtaatac catgcaacct
     3241 tgccgaaata tccacctgac ggatcacatc ataaacatcg tacacgccac ccgccatcac
     3301 ggcgcggtcg taactccctt tgcctaaatt cgtttccaac cattggttga tataagactg
     3361 taaacgataa tccatgcaat gaaccacaat tgcttcacac gtatgagaca tttcttccct
     3421 ccgaaaatta aatgatctga ctcgctgctc ttaacaagcg gtcatgaata gccagcccca
     3481 aaccttgttt tccaaaatca cgtgtcagaa tcaacttcac accttgatcg tccaacgctc
     3541 gcatgcccgc ataaagattc cgcgcaagcg cttcgaggtc attggatgca ccaagcggat
     3601 acggaatgca agcggactcg aaagacgcca tgtcttcatt tgcaagaaga agtcccacgc
     3661 ttcttccttc cg
//
LOCUS       DAOREF010000195         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15626_length_3671_cov_4.215155, whole genome shotgun sequence.
ACCESSION   DAOREF010000195 DAOREF010000000
VERSION     DAOREF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15626_length_3671_cov_4.215155"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKI33_05340"
     CDS             complement(<1..349)
                     /locus_tag="PKI33_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNM36462.1"
                     /translation="MRTALVDRERGRATYGVGGGLVWDSDASGEYAECLLKARVLTER
                     RPSFRLLETMLWEPDGGYFLLAAHLARLADTAVYFGVPIDRSAIEARLSELASTLSEA
                     SKIRLLVELDGAFT"
     gene            368..1039
                     /locus_tag="PKI33_05345"
     CDS             368..1039
                     /locus_tag="PKI33_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36463.1"
                     /translation="MSLRADETDGADPKRIVILIIIAIIALFVCTVVLIGGGWSILHR
                     SDLTPTATPISFGYCGAEFTSLCVVSFGRDALGNTVINLYVPIREYPVFYLKVVRQSG
                     EQRFECEWNRDVKESVYCTGTPLNLGEGFNIQIFSEKDDVLLAEGEFSLIAFRVTTPM
                     VGEAGSINTPSILNVESTPSVETEKTPTPSQSISESGTFTPTLSDSVVETQTLTPQSS
                     YPSYP"
     gene            1040..2806
                     /locus_tag="PKI33_05350"
     CDS             1040..2806
                     /locus_tag="PKI33_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36464.1"
                     /translation="MSVWSDGFSALSSQVWTVFIILILCLWGMLTIRIFLQRGLEKTL
                     TEIEIITLSAVGWIFPLLLLAIITFLVALFTNLVIAGVFVVLIFFSSTFLIVREKINV
                     LIGLKFLLFLIVVIVLRFAFLKNLFLPSYFDSAEHYRLISLIINAYKFGAVSSGLIDT
                     FYHLGFHFIAAFFSVFLQVRIIDFMLVFGQITLAFLSPALFFVVKRETGSSSSAVFTC
                     LLSTFGFHMPAYLMNWGKYPALLGLTAILFVLGLAYMIYRKDIFQVHKTFFIFLAGIF
                     LLSVFIHSRTFIVLGTMLIAILVTALWRRLNSLHRFIGFGVLILVISILLNSVKNSVV
                     LSPLLESYFRGDVWMLLLVLGLTFASLFHYAKQSFFLILWLTIMLLCMFVPVTLPRVG
                     VQTLLDRPFVQMFAVVPLSILGGLGSSALMELFARINVNQKIFRYLTPIILVGTVIMN
                     VALSYNFYSSSCCRFVTLDDLAAFSWMERSLPGEQKILVASSNLYVNPLDSGQTPVGV
                     DAGVWLSPLLSYETIFAERGISFDVKTTYTELCASNIDYIYVGGMPDSFNSAQLDNHP
                     NWYSSSFILPSARIYQVIGCER"
     gene            2842..>3671
                     /locus_tag="PKI33_05355"
     CDS             2842..>3671
                     /locus_tag="PKI33_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36465.1"
                     /translation="MKTFQQRDLFWGLPLSLIAGAALASIQPGVWLIGWMGFSFLLFL
                     SIFLLTSFTRWAGGGKALAWAVSLAFLLRLVGGVTTYLTLPIFGHAGDEEQSAGFTYT
                     DAYRRDAQAWDLAKSDRPILDAFSSRFAYDQYGGLLAFSAFIYRYLSPDAHRVLLLVL
                     ISALMGALGLPFLWRAISFQWDEKTALAAGWVFALYPESLLLGGVAMREPYLLAFSTF
                     CLWGFVDWNINREKKSLIWLALGIVGMLLVSPSVALVTLVILGGWLYFSSERGRFPWW
                     A"
BASE COUNT          822 a          768 c          826 g         1255 t
ORIGIN      
        1 cggtgaacgc gccatccagc tcgaccagca gccggatttt gctggcctcg ctcaaggtcg
       61 atgccagttc gctcaagcgg gcctcgatgg cggatcggtc gattggcacg ccgaaataaa
      121 ccgccgtatc cgccaaccgc gccagatgag cggccagcaa aaaatagccg ccatccggtt
      181 cccacagcat cgtttccagc agtcgaaacg agggtcgccg ctcggtcagg acgcgcgctt
      241 tgagcagaca ttccgcatat tcgccgctcg catccgaatc ccacaccagg ccgccgccca
      301 cgccatacgt cgcccgtccc cgctcgcggt cgaccagcgc ggtgcgaatg gcgacgctga
      361 actgcgcgtg tcgctccggg ccgatgaaac cgatggcgcc gacccaaaac gtattgtgat
      421 attaataatt attgcaatca tagctctgtt tgtttgtact gttgttttaa ttggcggagg
      481 gtggtcaata ttgcatcgaa gtgaccttac tccaactgcc actcctatat cttttggcta
      541 ttgtggtgca gaattcacaa gtttatgtgt ggtgtcattt ggacgcgatg cattaggtaa
      601 tacagtcatt aacttatatg taccaataag agaataccca gtcttttact tgaaagtggt
      661 tagacaatca ggagaacaaa gatttgaatg cgaatggaac cgtgatgtaa aagaaagtgt
      721 gtattgcaca ggtactccat tgaatttagg cgaagggttc aatattcaaa tcttttctga
      781 aaaagatgat gtcctccttg ccgaaggaga attttctttg atcgcatttc gcgttactac
      841 tccaatggtg ggagaagcag gttcgatcaa tacccctagc atcctcaatg tagaatcaac
      901 gccaagtgta gagacggaaa agaccccgac accatctcag tctatttctg aatctggtac
      961 ttttactcca acactatctg atagtgttgt tgaaactcaa acattgacac cgcaatcttc
     1021 ttatccaagt tatccataaa tgtctgtgtg gtctgatgga ttttctgcac tgtctagcca
     1081 agtttggact gtgtttatta ttctaatttt atgtctttgg gggatgctaa ccattagaat
     1141 tttcttacaa agaggattag aaaagactct gacggaaata gaaattatta ctctaagtgc
     1201 tgtaggctgg atatttcctc ttttattact tgctattatt acttttttgg tcgcactatt
     1261 tacgaatcta gttattgcag gcgtatttgt tgttctgata tttttttctt caacttttct
     1321 catcgttcga gaaaaaatca atgtattaat cggattaaaa tttcttttgt tcttgattgt
     1381 agttattgtt ttgcgttttg cctttcttaa gaatttattt ttgccatcgt attttgactc
     1441 tgcagaacat tatcgcctca tcagcttgat tattaatgca tataagtttg gtgctgttag
     1501 tagcgggcta atagatactt tctaccattt gggctttcac ttcattgcag cgtttttctc
     1561 tgtttttctg caggttcgta ttattgattt tatgcttgta tttggacaaa ttacgctagc
     1621 ttttttgagc ccagcccttt tttttgtggt taagagggag acaggatcaa gttcctccgc
     1681 tgtttttacc tgtttgttga gtacgttcgg ttttcatatg ccagcatatt tgatgaattg
     1741 gggaaaatat cctgctttat tgggattaac agcaatactt ttcgtcttgg ggcttgccta
     1801 tatgatttat cgtaaagata tatttcaagt tcataaaaca ttttttattt tcttggcagg
     1861 catattcctt ctttctgttt tcattcactc ccgaactttt attgttttag gcacaatgct
     1921 tattgcgatt cttgttacag cattgtggcg ccgcttgaat tcattgcatc gctttattgg
     1981 gtttggtgta ctcatacttg taattagtat attgctcaat tctgtgaaaa actctgtagt
     2041 attaagccct cttctcgaaa gctattttcg cggtgatgtt tggatgttgc tacttgttct
     2101 tgggttgaca tttgcttctt tatttcatta cgccaaacaa agcttttttt tgattttatg
     2161 gctgacaata atgttgctat gtatgtttgt tcctgtgaca ttacctagag taggggtgca
     2221 gactctccta gatcgccctt ttgtacagat gtttgccgta gttccgcttt ccattttagg
     2281 tgggttgggg tcttcagcat tgatggagtt atttgcgcgc atcaatgtca atcagaaaat
     2341 attccgatat ctgacgccta ttattctagt tgggacagtg ataatgaacg tcgcactatc
     2401 ttataatttt tactcgtcct cttgttgccg ttttgtgacg ttagatgatc tagctgcttt
     2461 ttcatggatg gaacgatcac tacccggtga gcaaaaaata ctagttgctt ctagtaattt
     2521 atatgtaaat ccacttgata gtgggcaaac accagtcggc gtcgatgcag gggtttggct
     2581 ttcgccattg ctttcttacg aaactatttt tgctgaacgt ggcatctcat tcgatgtaaa
     2641 aaccacctac acagagctat gtgcaagtaa cattgattac atttatgtag gtggaatgcc
     2701 tgacagtttt aattcggcac agctagataa tcatccgaat tggtattctt cttcttttat
     2761 tttgccttct gcaaggatat atcaagtgat tggatgcgaa cgctaaacac cagtagattg
     2821 tttcatggta aaagtggggc tatgaagact tttcaacaac gtgatctttt ttggggatta
     2881 cctctatctt tgattgcagg ggcggcgctt gccagcatcc aacctggagt atggctcata
     2941 ggttggatgg ggttttcctt tctacttttt ctttccattt ttcttcttac ctcattcaca
     3001 cgttgggcag gtggcggtaa ggctcttgca tgggcggtct ctcttgcttt tctgttgcgc
     3061 ttggttggcg gcgtaactac ttatctgact ctgcccattt ttggtcatgc tggggatgag
     3121 gaacagagcg caggcttcac ctacaccgat gcctatcgcc gcgatgcaca ggcatgggat
     3181 ctggctaaat cagaccgccc cattttggat gcattcagca gtcgcttcgc ctacgaccag
     3241 tatggcggtt tgcttgcctt tagtgcgttc atctaccgtt acctttcgcc agatgctcat
     3301 cgtgtcttac tactggtgct aatctctgca ttgatgggtg cactgggttt gccttttttg
     3361 tggcgagcaa tttcttttca gtgggatgaa aaaacggctc ttgccgccgg gtgggtcttt
     3421 gccttgtacc cagaaagttt gctactcggt ggagttgcca tgcgcgagcc gtacttgttg
     3481 gcatttagca cattttgttt gtggggattt gtagattgga atatcaatcg tgagaagaaa
     3541 tccttaattt ggcttgctct tgggatcgtg ggcatgttgt tggtttctcc atctgtcgct
     3601 ttagtgactc tggttattct tggcgggtgg ttatatttct ccagcgaacg cggacgtttt
     3661 ccgtggtggg c
//
LOCUS       DAOREF010000196         3667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15656_length_3667_cov_4.172481, whole genome shotgun sequence.
ACCESSION   DAOREF010000196 DAOREF010000000
VERSION     DAOREF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15656_length_3667_cov_4.172481"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKI33_05360"
     CDS             complement(<1..256)
                     /locus_tag="PKI33_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36466.1"
                     /translation="MNLREIEARLQTLIEVDLLKVLPGKRVEDVIVLKLATALQQNAI
                     QQDGTTVLPDVYTLILHPETAKAWEDEPFLTVLLNSVATVA"
     gene            complement(269..853)
                     /locus_tag="PKI33_05365"
     CDS             complement(269..853)
                     /locus_tag="PKI33_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNM36467.1"
                     /translation="MERKIFHGNIKPVDIAQALLAEFNQGNMRAQILGQSDKMVVQVG
                     TRPDAMSGGQTAMTVTIQTLEEGIMVELGQQAWLGVAASLGATALAAIRNPFSLLGRL
                     DDIAQDIENLQISERVWAVISKAARAAGASAELSENLKRTTCEYCHVANPVGQPSCSA
                     CGAPLGNVQPTTCRHCGFVVRPNEKTCPNCQKPV"
     gene            852..1514
                     /locus_tag="PKI33_05370"
     CDS             852..1514
                     /locus_tag="PKI33_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNM36468.1"
                     /translation="MNSIIIAFAILLSKLYEMTKYKFHPLVLTLTFLLLVVWIFNASF
                     PTNVHAQQPTDTPAPATGLFVTVVTNEPQINVRMGPSATVYPVIGVLLTGSTVPALGR
                     SKGGDWVQIEFASGPNGKGWVYSPLVEVSPAGSTLIVVDAPPTPVQPATSTIDPTLAA
                     QFIIEPTSTHLPTFTPAPTLIKVQFEPSASLSYSIPWASIIIALAVLGVIGFLLSLVI
                     RR"
     gene            1523..2149
                     /locus_tag="PKI33_05375"
     CDS             1523..2149
                     /locus_tag="PKI33_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36469.1"
                     /translation="MFDLRKRLIAFLFIIAFGMWMAFYQPTQNLVVAQQPTGSIPTVT
                     GTPPGMIVRVNIDQNFEIVPVHSGPNSTIYPVVGVILMGQEIPAVGISEDQDWIQIYY
                     PGVPGSTAWVFGPYVTIIKTGRLPVIPAPNTPTPVSTPTINPTLVAAFVDPVTPTRLP
                     TYTPPPEFVVPTFLPDTGSENRVPVGLFIIALGFIGGFGTLISFLRGR"
     gene            complement(2206..2616)
                     /locus_tag="PKI33_05380"
     CDS             complement(2206..2616)
                     /locus_tag="PKI33_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNM36470.1"
                     /translation="MLQEINTQLFTLTPAASEAVKNILTERKLDGYALRVYVAGGGCC
                     GVNFGMALDNNFRDVDTTFEANGVKVVVDEVSIDYLRDATVDFVNDPMRGAGFAVNSP
                     NAQGHSHGEGGCACGAGGGESSCGCGGGSCSCAN"
     gene            2809..>3667
                     /locus_tag="PKI33_05385"
     CDS             2809..>3667
                     /locus_tag="PKI33_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNM36471.1"
                     /translation="MFTMGLTSFEQIAIWAVFAVAWLGLGYAIFLRSQILNEDKGTEK
                     MQEVWGAIKDGANAYLQKQFKSILPLIGILFIALFLSVYVVPPSPEATERFSGMSESQ
                     VKLIIGLARALAFVMGAGFSLTVGQIGMRMAIEGNVRVAAASKKSFGDALRIAYRAGT
                     ITGMLTDGLGLLGGTIIFIILGIAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVGA
                     DLVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVSGLILGLALW
                     HATGRLEWIV"
BASE COUNT          838 a          955 c          852 g         1022 t
ORIGIN      
        1 gggctacagt tgccacagaa tttaatagaa ctgtaagaaa tggctcatct tcccaggctt
       61 ttgcggtttc agggtgcaat atgagcgtgt agacatctgg taaaacagtc gttccatctt
      121 gctggatcgc attttgttga agcgctgttg cgagttttaa gacaatcaca tcttccacgc
      181 gtttgcccgg caaaaccttt aaaaggtcta cttcgatcaa ggtttggaga cgtgcttcga
      241 tttctcgtaa attcattttg gtttttagtt atacaggttt ctgacagttc gggcaggttt
      301 tctcattagg tcttacaaca aaaccacagt gacggcacgt agtgggttgc acgttcccaa
      361 gcggagctcc acaagcgctg caggaaggct gaccaactgg gttggcaaca tggcagtatt
      421 cacaagtagt gcgtttcaaa ttctcagaga gttcggcaga tgcaccagcc gcacgagccg
      481 ccttggagat aaccgcccaa acacgttcgc tgatttgtaa attttcaatg tcttgagcaa
      541 tgtcgtccaa gcgtcctaat aagctgaacg gatttcgaat agcggcaagc gcagtggcac
      601 ccaagctggc agccactcca agccaagctt gttgacccaa ctccaccata atgccttctt
      661 caagtgtttg gatcgttaca gtcatcgcgg tctgcccgcc agacatagca tctgggcgcg
      721 tgcccacctg cacaaccatc ttgtcactct gacccaagat ctgcgcacgc atattcccct
      781 gattgaactc tgccagcaaa gcctgtgcaa tatccaccgg cttgatattc ccgtgaaaga
      841 tttttcgctc catgaactcg attataatcg cctttgcgat tctcctttct aaactttacg
      901 aaatgacaaa atataagttt catcccctcg tactgacgtt aacctttctt ttattggttg
      961 tttggatatt caacgcctct ttcccgacta atgtccatgc tcaacaaccg acggatactc
     1021 ccgcccctgc taccggtctc tttgttactg tagtaaccaa tgaaccgcag atcaatgttc
     1081 gcatgggtcc cagcgcaacc gtgtatcccg tgattggtgt gttattaacc ggctcaacag
     1141 tcccagcttt ggggcgttca aaaggcggcg actgggttca gatcgaattt gcatccggac
     1201 ctaacggaaa aggctgggta tattccccgc tggttgaggt ctcaccagca ggtagcaccc
     1261 tcattgttgt agacgctcca ccaacaccag tccaacctgc cacatccact attgacccga
     1321 ctcttgccgc gcaattcatt attgagccaa ccagcacaca tctgccgacg tttacccctg
     1381 caccaaccct catcaaagtt caatttgaac catctgccag cttgagctat agcattccgt
     1441 gggcttcgat cattattgca ttggcggtct tgggtgtgat tggcttttta ctttctttgg
     1501 tgatccgtcg ttgaaattga ccatgttcga ccttagaaaa cgtttgatcg cctttctatt
     1561 tataatagca tttgggatgt ggatggcgtt ctaccagcca acacaaaacc ttgtcgttgc
     1621 acaacagccc acaggttcaa ttccaaccgt aaccggtaca cctcctggca tgatcgtacg
     1681 tgtgaacatt gaccagaatt tcgagattgt acctgttcat tcaggtccga actcaacgat
     1741 ctaccccgtt gttggggtta ttcttatggg gcaagagatt ccagccgtag gcatttctga
     1801 agatcaagat tggattcaga tttattaccc cggcgtaccc ggctcaaccg cctgggtctt
     1861 tggaccatac gtcaccatca tcaaaacagg acgactacca gtcattccgg ctccaaatac
     1921 gccgacgcct gtttcaacac ccacaattaa ccctactctg gttgctgctt ttgttgaccc
     1981 cgtcacccca acgcgtttac caacttacac accgccgcca gaattcgtcg tcccaacatt
     2041 cctgccagat acaggttccg aaaaccgtgt gcctgttggc ttattcatca ttgccctcgg
     2101 gtttattggc gggtttggca ccttgatctc atttttacgt ggtcgataaa aaggaacaaa
     2161 aaaggacagg tctatgacct gtccttttga tttaacgatc ttagattaat ttgcgcagct
     2221 gcaagaaccg ccgccgcatc cgcaagagct ttcaccgcca ccagcaccgc aggcgcaacc
     2281 gccttcgccg tggctgtgtc cctgagcgtt cgggctattg acagcgaaac cagcgccccg
     2341 catcgggtcg ttgacgaaat caacagttgc atcgcgtaag taatcgatgg aaacttcatc
     2401 aacaacgacc ttgacgccat tggcttcgaa ggtggtatcc acatcacgga aattattatc
     2461 aagcgccata ccaaagttga cgccgcagca accaccgcca gcaacgtaca cgcgcaacgc
     2521 gtaaccgtct aatttgcgtt cggtcaaaat attcttaacc gcttcgctgg cagcaggggt
     2581 aagtgtaaaa agttgggtgt taatttcttg gagcataagg tttctccttg tgttacatct
     2641 aattttggta aatgttatca ggtttaaagt gggctgtcaa ctttttttgt atgaagatta
     2701 atccaaactc attttcttga cacaaagact atgcgactgt aaaatctctg tgcttttaaa
     2761 aaggcgtcag cctttatttt ttcatctctg ttggaggagt atcctttcat gttcacaatg
     2821 ggattgacca gttttgaaca gatcgcgatt tgggccgttt ttgccgttgc atggcttggg
     2881 ctcggatacg cgattttttt gcgctcccag atcctcaatg aggataaggg aacggaaaaa
     2941 atgcaagaag tttggggtgc gattaaagat ggcgcaaatg cctatttgca gaagcaattt
     3001 aaatccatct tgcctttgat cggcatcctc ttcattgcct tgttcctttc cgtgtacgtg
     3061 gttccgccat ctcctgaagc gactgaacgc ttcagcggta tgagcgaaag ccaggtcaag
     3121 cttatcatcg gtcttgcacg tgctctggca tttgtcatgg gtgcgggctt ctcactgacg
     3181 gtaggtcaga tcggtatgcg tatggcgatt gaaggaaatg tgcgtgtagc ggcggcttcc
     3241 aagaagtcgt ttggcgatgc gcttcgtatt gcttaccgtg ctggcaccat caccggtatg
     3301 ctcaccgatg gtctcggtct gctcggcggt accatcatct ttattatcct tggtatcgca
     3361 gcccccgatg ctctgctggg cttcggcttt ggtggcacgc tcctcgcgtt gttcatgcgc
     3421 gtgggcggtg gtatctttac caaggctgcc gatgttggtg ctgacctcgt cggtaaagtc
     3481 gaagcgggta tccctgagga tgatccacgc aaccctgctg tgatcgcgga tctcgttggt
     3541 gataatgttg gtgactgcgc tggtatggct gccgacatct tcgaatccta tgaagtgacc
     3601 atcgtttctg gtttgatcct cggcttggct ctctggcatg ctacgggtcg cctcgaatgg
     3661 atcgtct
//
LOCUS       DAOREF010000197         3661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15697_length_3661_cov_4.534942, whole genome shotgun sequence.
ACCESSION   DAOREF010000197 DAOREF010000000
VERSION     DAOREF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15697_length_3661_cov_4.534942"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKI33_05390"
     CDS             complement(<1..209)
                     /locus_tag="PKI33_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNM36472.1"
                     /translation="MAKNKTHTRYVCQQCSRVSASYMGKCPQCGAFDSMVEEVVHDEP
                     VAKKGAVNVRGLTGRSEPRPISEVSG"
     gene            345..692
                     /locus_tag="PKI33_05395"
     CDS             345..692
                     /locus_tag="PKI33_05395"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNM36473.1"
                     /translation="MKLVSDILIVIVALLHFFFLYLEMFLWDKPRGMKSFNLTEEFAK
                     QSKRLAMNQGLYNGFLAAGLLWGLFAGNPVKIFFLSCVIIAGIFGAFTVSKRIFYIQA
                     VPAIVALVILLVA"
     gene            complement(694..2232)
                     /locus_tag="PKI33_05400"
     CDS             complement(694..2232)
                     /locus_tag="PKI33_05400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM36474.1"
                     /translation="MTPPNILYSTALLTAGICCFIVGLLVLQRRPFMPGSIPLMVFIL
                     AMCWWDISYGIFWGGVLKPSPYFWLDISYIGVVIVPAAFFVFAMQISGRQEWLKTPLL
                     IGLYVEPLLVLVLMWTDPWHNLFFAGKRLENTGMILNAGPVFWANVVYSYLLLLVTFI
                     LLVRRFTTTSGIYRRQLGVVIAGAGVTWLNSFIFVTGLSPFPNADNTPFSFTIAAFAF
                     AFALLRYRMLDIVPVARDTLIEGMSDGVLVLDTNNRIVDMNPEAEKLLSASSAGTIGR
                     HVEEVFAPWADLVKTFMNVDQTRVEVLASRDSDTYLDLQISPLFDKRKNFIGRLVMWR
                     DISRLKKIQTELEKLATQDPLTHVYNRRHFYTLAEVEIHRSLRSQLPFSVILVDIDYF
                     KSINDKYGHKAGDEALIHFTKICIQNLRKVDIFARFGGEEFVILLPEADLDQAKETAE
                     RIRKIVESTVLNIDEHQIKITASFGVSTFKGSPDTLDLLLQKSDEALYAAKNTGRNRV
                     VCAD"
     gene            complement(2311..3345)
                     /locus_tag="PKI33_05405"
     CDS             complement(2311..3345)
                     /locus_tag="PKI33_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNM36475.1"
                     /translation="MHYNIALIGFGNVARSLARLLVRKQDVLKSKYDVTFSFTGISTG
                     RHGYAVNQSGLDIQKALDLVESGKDISELSVFPVNDSISVINNSAANVMFENSPVNTQ
                     TGQPAVDHIRTALNLGMHAITANKGPVVHGYRELTALAESKGKTFRHESAVLGGAPVF
                     SVMREAFPLADLESFKGIFNATTNVILSRMESGETYEQALAYAQKIGLAETDPTNDVD
                     GWDAAIKVASLVTVLWDTPMTPQQVNPTGIRGITSEMIANAKKEGKRYKLVCSAERTD
                     GKIVASVSPQLVDSSSSLYGMMNSSTGITFRTDVIIDYSIVLSEKPGMTGGPVETAYG
                     LFADFVNIVR"
     gene            complement(3402..>3661)
                     /locus_tag="PKI33_05410"
     CDS             complement(3402..>3661)
                     /locus_tag="PKI33_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36476.1"
                     /translation="YAIDHGICFHEENKLRTVLWDFAGQAIPDELLAPLSLTGNWSAL
                     FEPYLSPREVDALLFRAEELCEAKVFPRPLQGRRAYPYPPI"
BASE COUNT          987 a          905 c          860 g          909 t
ORIGIN      
        1 ccgctgacct cagaaatggg gcgcggctcc gagcgcccgg tcaaccctcg aacattgacc
       61 gcgcctttct tcgccaccgg ttcatcgtgg acaacttctt ccaccatgct gtcgaaggct
      121 ccgcactgcg ggcatttgcc catgtacgaa gcagagactc gactgcattg ctgacagaca
      181 tatcgggtgt gggttttgtt ttttgccatt agatacctct tgtaggggcg agtcgcgact
      241 cgtccctaca aatcatggtc gcagtataac agaatttttg ttctgttttc agggtctgct
      301 atactcacag gtgaaaatca cctcattcgc ccaaggagaa ctttatgaaa ctcgtatcag
      361 acattcttat tgttatcgtc gccctcttgc acttcttctt cctctacctc gaaatgttcc
      421 tatgggacaa accccgcggc atgaaatctt tcaatctcac cgaagagttc gccaagcaat
      481 cgaaacgtct cgccatgaat cagggactat acaatggctt cctcgccgcc ggtttgctgt
      541 ggggattatt cgctggcaac ccggtgaaaa tcttcttcct cagttgtgtc atcatcgcag
      601 gaatcttcgg cgcattcacc gtcagcaagc gcatctttta cattcaggct gtccccgcta
      661 ttgtagcctt ggtgatttta ttagtcgcat aagttagtct gcacagacga cccgattgcg
      721 tcctgtattt tttgcagcat acaaagcttc atctgatttt tgcagcaaaa ggtctaaggt
      781 gtcggggctg cctttaaagg tggaaacacc aaaactggca gtgatcttga tttgatgttc
      841 atcaatgttc agcactgtgc tttccacaat ctttcgaatt cgctcggctg tctctttggc
      901 ttgatccaag tccgcttcgg gcagcaagat gacaaattct tcgccgccaa atctggcaaa
      961 tatatccact ttgcggaggt tctgtatgca gatcttcgta aagtggatta gtgcttcatc
     1021 gccggcttta tgtccgtatt tatcgttaat gctcttgaag taatcaatgt ccaccaaaat
     1081 gacagagaag ggcaactgag aacggagaga gcggtgaatt tccacttctg ccaaggtgta
     1141 aaaatgacgt cggttgtaga cgtgggtcag cgggtcttgt gtggcaagtt tttcaagctc
     1201 ggtttggatt ttcttcaagc gtgaaatatc acgccacatt accaaacgtc cgataaagtt
     1261 ttttcgttta tcgaacaatg gggaaatttg aaggtcaaga tatgtatctg agtcacggct
     1321 ggctagaact tccacccggg tttggtccac gttcataaat gtttttacaa ggtcagccca
     1381 cggggcgaaa acttcttcta cgtgcctgcc aatcgtcccg gcggaagagg cggagagaag
     1441 tttttccgct tcgggattca tatccacgat acgattgttt gtatccaaaa caagcacacc
     1501 gtcgctcata ccttcgatca atgtatcgcg cgccacgggc acaatatcca gcatgcgata
     1561 acgaagcaaa gcaaaggcaa acgcaaaggc agcgattgta aatgaaaagg gcgtgttatc
     1621 tgcatttggg aaagggctta aaccagtgac aaaaataaaa ctattgagcc aggtaactcc
     1681 tgcgcccgca atcacaaccc caagctggcg gcgataaatt ccagaagtcg ttgtaaatct
     1741 acgcactaac aaaatgaacg tgaccaataa caacagataa gaatatacaa cgtttgccca
     1801 aaataccggt cctgcattta agatcatgcc ggtgttttcc aaccgtttgc cagcaaagaa
     1861 aagattatgc caggggtcgg tccacatcag gacaagaacc aacaacggct ctacatacaa
     1921 cccaatcaac aatggtgttt tcaaccattc ttgcctgcct gagatttgca tggcaaaaac
     1981 aaaaaacgcc gccgggacga taaccacccc gatataacta atatccaacc aaaagtaagg
     2041 tgaaggcttt aaaacgccgc cccagaaaat gccatacgaa atgtcccacc agcacatggc
     2101 aaggatgaaa accatcaatg ggatcgaccc cggcataaac gggcgtcttt gcaacaccaa
     2161 taaaccaaca ataaagcaac aaatcccagc cgttaagagt gccgtactat acaagatgtt
     2221 tggaggggtc ataaaagcct ttgtgtctac tattgtaaac acaaaggctc tactttacta
     2281 cttaacaatg atattcgaat aaaagggcag ttagcggacg atattcacaa aatcggcaaa
     2341 gagaccgtat gcggtttcca cgggtccacc cgtcataccg ggtttttcag agaggacaat
     2401 ggagtaatca ataatcacgt ctgtgcggaa ggtgatgcca gtgctggaat tcatcatgcc
     2461 atacagagac gaagatgaat cgaccaactg cggtgaaaca ctggcaacaa tcttgccatc
     2521 cgtcctttca gctgagcaaa ccaacttata gcgtttgcct tctttcttgg cgttggcgat
     2581 catctcactg gtaatgccac gaatgccagt gggattcact tgctgcgggg tcatgggcgt
     2641 atcccataac accgtgacaa gcgatgcaac cttgatagcg gcatcccagc catccacgtc
     2701 gttggtgggg tcggtttcag cgagaccgat cttctgtgcg tacgcaagtg cctgttcata
     2761 cgtctcgccg ctttccatgc gagataagat gacattggtc gtagcattga agataccttt
     2821 gaaggattca agatccgcga gcggaaacgc ctcacgcatg accgagaaaa cgggtgcgcc
     2881 tcccaaaaca gcagactcgt gccggaacgt tttgcccttc gattccgcta acgcggtcaa
     2941 ctcgcgatag ccatgcacaa ctggaccttt atttgcggtg atagcatgca ttcccaaatt
     3001 caacgccgtg cggatgtggt ccacggcggg ttgaccggtc tgggtattga cgggagaatt
     3061 ttcaaacatc acattggcag cagagttgtt gatcacgctg atcgagtcat tgacagggaa
     3121 tacagaaagc tcagagatat cttttccgct ttcgaccaag tccaaggctt tttgaatatc
     3181 caacccgctt tgattgacgg catatccgtg acgaccggtt gaaatgccag taaatgaaaa
     3241 ggtgacatca tatttcgact tcaagacatc ctgcttgcga accagcagac gagccaagga
     3301 acgggcgaca tttccaaaac cgatgagtgc gatgttgtag tgcataaaaa tttctcctga
     3361 ttaaatttcg taggggcgac ccagcaggtc gcccctatag tttatattgg cgggtaagga
     3421 tacgcccttc gtccttgtaa cgggcgtggg aagacttttg cctcacacaa ttcttctgca
     3481 cggaaaagta gggcgtcgac ttcgcgtggg ctgagataag gctcaaagag ggcagaccag
     3541 tttccggtca aagagagagg cgcgagcaat tcatccggaa tggcttgacc ggcaaaatcc
     3601 cataacacgg tgcggagttt attctcctca tgaaagcaaa tgccatggtc gatggcatag
     3661 a
//
LOCUS       DAOREF010000198         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15714_length_3659_cov_3.582131, whole genome shotgun sequence.
ACCESSION   DAOREF010000198 DAOREF010000000
VERSION     DAOREF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15714_length_3659_cov_3.582131"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..558)
                     /locus_tag="PKI33_05415"
     CDS             complement(154..558)
                     /locus_tag="PKI33_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36477.1"
                     /translation="MLFIALIVCILISTGSFVWGYSQAGYEDAVRWLVAFGVLWFLAE
                     WRKWKWFSAPAVLFSLFLAGYGVWFKFVPGLMFNGAVFSVLAWNITEFQEKLRLLPQR
                     EDKQGMTRRHLIRIGTLSVGAVLIALFLGIRG"
     gene            616..1155
                     /locus_tag="PKI33_05420"
     CDS             616..1155
                     /locus_tag="PKI33_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNM36478.1"
                     /translation="MPKEQEINSIERTAEECQGKLHPKAIVGLQLFNQGEFFEAHEEL
                     ETAWRDETSSIRDLYRGILQIAVTYLHITRGNYDGAVKVYGRSLKWLQGWGDVCRGVN
                     IKKLRRDAETVMQEVTRLGRERIREFDRSLFQPIQWNEKRLWICDRCGSEMYEKNCKV
                     SCPTCGNRFDCSDLNLYFD"
     gene            1171..1938
                     /gene="fabL"
                     /locus_tag="PKI33_05425"
     CDS             1171..1938
                     /gene="fabL"
                     /locus_tag="PKI33_05425"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNM36479.1"
                     /translation="MNFQNKIALVTGSGRGIGKAIALNFASQGADIVVNFFRNRAPAE
                     ETVREIEKLGRRALLVKADVGDLDDLNRLFDEVEKEFGGLDIFIHNAASGYNRPAMEQ
                     KPKGWDWTMNINARALLFAAQRATPLMEKRGGGSIVSISSAGSVRVLPDYVVVGASKA
                     ALEALTRYLGVELIQRGIKVNAVSPGVVETEALHHFSSMGGQENILQKFIERVPAGRL
                     ITPEDVAEVVAFLCSPASSMIVGQTIVVDGGYTLPIQ"
     gene            1950..2699
                     /locus_tag="PKI33_05430"
     CDS             1950..2699
                     /locus_tag="PKI33_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNM36480.1"
                     /translation="MFQAAPPTRFDLQFSIAGIPVRVHPLFWVIAILLGSGSSNLLMI
                     PVWIAVVFISILVHELGHAFAFRRFGQPSRILLHFSGGLTIPESMPWGGGYATVSLTP
                     NQHIFVSLAGPFAGFLLAGLILAVGVAMGGTLIFSTILKIIPFPIVMMPYGSGILTDI
                     FVTFLWVNIFWGIINLLPVYPLDGGNVARYLLIQRDPWNGLSTSLWVSVITGGLLAVV
                     GILLLKSVYMAFLFGLLAFQSYQMLQASGRY"
     gene            complement(2746..>3659)
                     /locus_tag="PKI33_05435"
     CDS             complement(2746..>3659)
                     /locus_tag="PKI33_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNM36481.1"
                     /translation="NGSYGAVLYSNQGSVFVTDSTFDLNTNSGLVAVSGTGIELVNVT
                     ADGNGGDGVEVYSSYTWGCRGANDTPVNIVVNVDGGTFTNNTGYGIFAKPGPQGDMVF
                     VTPSTFGGNGLGDYLTDLSDPEICPPQEEEEQDNPNVVPAPSNPPVEQDCVNFTSTIL
                     ELENGTWVKVGCPYEGFSNLDSILEGQLPGPLGAGVKFLNGIILSLTDADGNTILNQD
                     GTVTVNLLIPEGSRGRTHSVLFWDETLNDGKGGWMKLPPYEAGTSFPLHPENPDDPRL
                     IVSGVQQVDNMVTFTINFSGTFILTTP"
BASE COUNT          951 a          875 c          851 g          982 t
ORIGIN      
        1 tggtctgaag agtctttgtt tcattggctg ttcaaaacct attggcgcag aaaacgtgaa
       61 atccccatct tgttatcaaa gcctgaaaat cgacacctca caatattgca ttttcgccac
      121 tcacaagaag cggaagcatg gctaaagggt ttgtcaccca cgaatgccta gaaaaagcgc
      181 aatcaagacc gcacccacag aaagagttcc aatgcgtatc aagtggcgtc ttgtcatacc
      241 ttgtttgtct tctcgttgag gtagtagcct caatttttct tgaaattcag taatattcca
      301 tgccaataca ctaaacacag caccgttaaa cataagccca ggaacaaact taaaccaaac
      361 gccataccca gctaaaaaca gactaaacag cacagccgga gcagagaacc acttccattt
      421 acgccattcc gccaaaaacc aaagaacacc gaaagctact agccagcgga cggcatcctc
      481 atagccagct tgagagtatc cccatacgaa cgagcctgtt gatatcaaga tgcagacgat
      541 aagcgcaata aataacaatt ttcctccaac acaaatttta ccattcgtat aaaacctgca
      601 tgttatactc tcaatatgcc aaaggaacaa gaaataaatt ctattgaaag aacagcagaa
      661 gaatgccaag gaaagttaca tccgaaagcc attgtaggtc ttcagttatt caatcaagga
      721 gaattttttg aagcccatga agagttggaa accgcttggc gagatgaaac cagctcaatt
      781 cgagacttat atcgcggcat tcttcaaatc gcggtaacct atctgcatat tacacgcgga
      841 aactatgacg gtgcagttaa agtctatgga cggagcttga agtggttgca aggctggggc
      901 gacgtctgcc gcggtgttaa catcaagaaa cttcgccgtg atgcagagac cgttatgcaa
      961 gaagtgactc gtttggggcg ggaaagaata cgtgagttcg ataggtcttt attccaacct
     1021 attcaatgga acgaaaagcg cctgtggatc tgcgatcgct gtggcagtga gatgtatgaa
     1081 aagaattgca aagtgagttg cccaacctgt ggcaaccgct tcgactgttc ggatttaaat
     1141 ctttactttg attaatgctc tggagaccca atgaattttc aaaacaaaat cgccctcgtc
     1201 accggctcag ggcgcggaat tggaaaagcc atcgccctga attttgccag tcagggggca
     1261 gatattgtcg ttaatttctt tcgcaaccgt gctcctgccg aagagacagt tcgcgagatt
     1321 gaaaaacttg gcaggcgtgc tttgctggtt aaagccgatg taggcgattt agatgactta
     1381 aatcgcctct tcgatgaagt agaaaaagaa tttggcggct tggatatctt cattcacaat
     1441 gctgcatcag gctacaaccg ccctgcaatg gaacaaaagc ccaaaggctg ggattggaca
     1501 atgaacatca atgcgcgtgc attgttattc gctgcacagc gtgccacgcc tcttatggaa
     1561 aaacgcggcg gtgggagcat cgtcagcata tcgagtgcag ggtcggtgcg agtgttaccc
     1621 gattatgttg tggtgggcgc cagtaaggca gccctggagg ccctcacgcg ctatttaggt
     1681 gtggagttga ttcagcgtgg catcaaggta aacgcggtgt cacccggcgt ggtggagaca
     1741 gaggcgttgc accatttttc atccatgggc gggcaggaaa acatcctcca gaaatttatc
     1801 gagcgcgtac cggctggtcg tctcatcact cctgaagacg ttgcagaagt ggtagcgttt
     1861 ttgtgttcgc ccgcttcttc catgattgtt ggtcaaacca tcgtcgtgga tggcggctat
     1921 actttaccca ttcaataaca ggacatatta tgtttcaagc tgctcctccc actcgttttg
     1981 atttacagtt ttcaatcgcc ggcattcccg tgcgcgtgca tccgctcttt tgggtgattg
     2041 ctattttgct tggctctggc tcaagcaatt tgttgatgat cccagtttgg atcgcagtag
     2101 tttttatttc tattcttgtt catgaacttg gtcatgcatt tgcgttcagg cgctttggtc
     2161 aaccatcgcg cattctcctg catttttctg gtggattaac catccccgaa tcgatgccat
     2221 ggggcggcgg atatgccacc gtgagcctga cacccaatca acacatcttc gtctcacttg
     2281 ccggaccgtt tgccggattc ttgctcgcag gcttgatcct cgctgtgggt gttgcaatgg
     2341 gcggcacatt gattttctct accatactaa aaatcatccc cttccccatc gtcatgatgc
     2401 catacggcag cggtatcttg acagatatct ttgtgacttt tttgtgggtc aatattttct
     2461 ggggcatcat caatctcttg ccggtttatc cactggatgg cgggaatgtt gctcgttatc
     2521 tgctcatcca acgcgacccg tggaatggct taagcacttc actatgggtt tcggtcatca
     2581 ccggcggttt gctcgctgtt gtaggtattc ttttactcaa aagtgtttat atggcgtttc
     2641 tatttgggct tttggcattt caaagttatc aaatgctgca agcctcagga aggtattaag
     2701 cacaaaagga cggctctgtt ttgagccgtc cttttgtttg tgtccttatg gggttgtaag
     2761 aataaaagta ccagagaagt tgatggtaaa ggtcaccata ttatctactt gctgaacacc
     2821 tgaaacaata agtcgaggat catctgggtt ttccgggtgg agcgggaagc tggtgcccgc
     2881 ttcataaggc ggcaacttca tccaaccgcc cttgccatca ttcaaagtct cgtcccagaa
     2941 caaaacagag tgagttctac cgcgtgaacc ttcggggatc aagaggttga ccgttactgt
     3001 cccatcttgg ttgaggatcg tgttcccgtc tgcatctgtt aggctgagaa tgattccatt
     3061 taggaacttc actcccgccc caagcggacc aggtaactga ccttcaagaa tgctatctaa
     3121 attgctaaag ccttcatagg ggcaaccgac cttaacccag gttccatttt caagttccag
     3181 aattgtgctg gtgaagttga cgcaatcctg ttccacaggc gggttggaag gcgcaggaac
     3241 gacattgggg ttatcttgtt cctcttcttc ttgtggcgga caaatttctg ggtcgcttaa
     3301 gtcagtcaga taatcgccca aaccgttccc accaaatgtg gaaggtgtta cgaagaccat
     3361 gtctccttga ggacctggtt tagcaaagat tccataacca gtgttgtttg taaatgtacc
     3421 gccatctaca tttacaacga tgttgactgg agtatcattg gcaccacgac agccccacgt
     3481 ataagaacta taaacttcga cgccatctcc gccatttcca tcagccgtca cattgaccag
     3541 ttcaatgcct gtgccactga ctgccaccaa gccagaattt gtgttcaagt caaatgtact
     3601 atcagtaaca aaaacactgc cctggttaga atagagaacg gcaccataac ttccattgg
//
LOCUS       DAOREF010000199         3656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15737_length_3656_cov_4.880589, whole genome shotgun sequence.
ACCESSION   DAOREF010000199 DAOREF010000000
VERSION     DAOREF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15737_length_3656_cov_4.880589"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /gene="gltX"
                     /locus_tag="PKI33_05440"
     CDS             complement(<1..1029)
                     /gene="gltX"
                     /locus_tag="PKI33_05440"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNM36482.1"
                     /translation="MSIQPARTRFAPSPTGHLHLGGARTALYAYLLAKKTGGQFILRI
                     EDTDLKRTVPGAEQEIMDGLRWLGLNYDEGPDIDGAYGPYRQTERREIYQAHAKTLVE
                     KGHAYPCFCTPERLDKVRKEQMDRKENPRYDGTCRNLAPDEAAKRIANGEAYTIRFKI
                     PQDGVTVAHDHLRGDITIENKQLNDQVILKTDGLPTYHLAAMVDDHEMKITHVMRGSE
                     WLGTFPLHVNIVRAFGWDEPIWIHLSVFLKPSGKGKMSKREAADAMKDGYSIFVKDMQ
                     DLGFTPEGVLNWCGLMGWGVAEDDVMTTAQMVDRFTIDSLTPSPAAINFQRLDHFNAT
                     HIRLLTTED"
     gene            complement(1049..2137)
                     /locus_tag="PKI33_05445"
     CDS             complement(1049..2137)
                     /locus_tag="PKI33_05445"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNM36483.1"
                     /translation="MLGAVILASAFGVVMIRSAIAGNEVLAISSSRQIYFAMLGVVLI
                     FFLASIDYRYWLALDRPIHLVILGFLVTLTGFGQTAFGAQRWFQVGVLFLQPTEFAKI
                     VALIVLAKYFDSVRDQPRDLRWIFGAILRAMWIIGLILAQPNLSNVMVLSVILAVMLW
                     VNGIQVKHVITAVLLGALAVISVIALSVLGVRIPFLQAYQQERIVNFVVPDPNDTYGN
                     RYNVQQALIAVGAGGFSGEGYGYGTQTQLRFLKVRHTDFIFAATSEEFGFVGSVSIIL
                     LLGVVVWRCIRAAQKARDVAGAMLAIGVATLIFFQGAVNIGMNLNLLPVSGLPLPFVS
                     YGGSGLTALMIAIGLVESVVMRQKDLEF"
     gene            complement(2239..2490)
                     /gene="minE"
                     /locus_tag="PKI33_05450"
     CDS             complement(2239..2490)
                     /gene="minE"
                     /locus_tag="PKI33_05450"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNM36484.1"
                     /translation="MDFFTRKRSATSAKERLQLVLVHDRTDLTPGQLESLKDELLKAI
                     SKYIEIDPEAVRIELEKDGREQRLVADIPLKGATRHRAG"
     gene            complement(2492..3289)
                     /gene="minD"
                     /locus_tag="PKI33_05455"
     CDS             complement(2492..3289)
                     /gene="minD"
                     /locus_tag="PKI33_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNM36485.1"
                     /translation="MTAQVITVTSGKGGVGKTTAVANLATALAMDGKRVVCIDGDIGL
                     RNLDVVMGLENRIVYDIVDVIEGRCKLKQAMIRDKHYQDMYLIPAAQTRDKNAVSPSD
                     MIRICNDLRPDTDFIVIDSPAGIERGFRNAIAAADSVLVVTNPEVSAVRDADRVVGIL
                     EAEEKGNPALIINRLNPALVKNNDMLSAEDVLDLLGIRLIGIVPEDENVIIGSNRGTP
                     IAADGKSRAGQAFRNIAKRLQGVDVPFMDLTPQSGLWNTIQRLAGRK"
     gene            complement(3291..>3656)
                     /locus_tag="PKI33_05460"
     CDS             complement(3291..>3656)
                     /locus_tag="PKI33_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNM36486.1"
                     /translation="HEAVSSDTALFVSKTVRSGTRIEYPGTIVVFGDVNPGAEIVAEG
                     NVIVWGRVRGVIHAGVKGDRSAFICALDLSANQLRIANESSAMLKPQKDPKPEIASIN
                     SEGKLQAELWQTGETLIRK"
BASE COUNT          992 a          961 c          819 g          884 t
ORIGIN      
        1 gtcttcggtc gtcaggaggc ggatgtgagt cgcgttgaag tggtcgaggc gctggaagtt
       61 gatcgcagcc ggagaaggcg tcaacgagtc aatggtgaag cgatccacca tttgagccgt
      121 ggtcatcaca tcgtcttcag ccacacccca acccatcagc ccacaccagt tcaaaacacc
      181 ttcgggagtg aaacccaggt cttgcatgtc tttgacgaaa atcgaataac cgtctttcat
      241 ggcatctgcc gcttcacgtt tggacatttt gcccttgccg cttggcttga gaaaaacgga
      301 aagatggatc caaatcggtt catcccaccc aaaggcgcgg acgatgttga catgcaacgg
      361 gaaggtgccc agccactctg agccgcgcat cacatgcgtg atcttcattt catggtcgtc
      421 caccatggca gcaagatgat aggtcggcaa gccatctgtt ttgagaatga cttgatcgtt
      481 gagttgtttg ttctcgatag taatgtcgcc gcgcaggtgg tcatgtgcaa ctgtcacgcc
      541 atcctgcgga attttaaaac gaatggtata tgcctcacca ttggcaatgc gctttgcggc
      601 ttcatcagga gctaggttgc ggcaagtccc gtcataacgc gggttttcct tgcggtccat
      661 ctgctccttg cggactttat ccaaccgttc aggcgtgcag aaacaagggt atgcatgccc
      721 tttttctaca agcgtttttg catgggcttg ataaatttct cgcctctcgg tctgacgata
      781 gggcccgtat gccccgtcaa tatctggacc ttcatcgtag ttcagtccaa gccaacgcag
      841 accatccatg atctcttgtt cagcaccggg gacagtacgc ttcagatctg tatcttcgat
      901 acgcaaaatg aactgtccgc cggtcttttt ggctaacagg taggcataca aagctgtacg
      961 tgcaccaccg aggtgcaaat gcccggttgg cgagggtgca aagcgagtgc gagctggctg
     1021 aatggacaaa atttcactcc tacttaattt agaattccaa gtctttctga cgcatgacga
     1081 cggattctac caaaccaatg gcgatcatca gcgccgtcag accactgcca ccgtaactta
     1141 caaatggtaa cggcaaacca gaaacaggca ataagtttag gttcatacct atattcaccg
     1201 ccccctgaaa aaagatcaat gttgctacgc caatagcaag catggctcct gcaacatcac
     1261 gcgctttttg tgccgcacga atacagcgcc aaacaaccac acccaacaaa aggatgatcg
     1321 atacagaccc tacaaagcca aattcttctg atgttgccgc aaagataaag tctgtatggc
     1381 gcactttaag aaagcgtaat tgtgtttgtg tgccgtaccc atacccttcg ccagaaaaac
     1441 cgccagcccc cacagcgatc agagcttgtt gcacgttata acggttacca taggtatcat
     1501 ttgggtcggg aacgacgaag ttaacgatac gctcttgttg atatgcttgt aaaaacggaa
     1561 tccgtacacc aagcactgaa agggcgatca cactaatcac cgctaaagca cccaacagca
     1621 cagctgtaat aacatgcttt acttgaatcc catttaccca aagcatgact gctaaaatga
     1681 ccgaaagaac catcacatta cttaagtttg gctgcgctag aatcaaaccg atgatccaca
     1741 tggcgcgcag aatagcacca aaaatccaac gtaaatcacg tggttggtca cgaactgaat
     1801 caaagtactt ggcaagcaca atcaatgcaa caatcttggc aaactcggta ggctgcagaa
     1861 agagcacacc tacttgaaac cagcgttggg caccgaatgc ggtttgacca aaaccagtca
     1921 gtgtgacgag aaagcctaga ataacaaggt gaattggacg gtcaagcgcg agccaatacc
     1981 gataatcaat agaagcgagg aagaaaatca acactacgcc aagcatggca aagtaaatct
     2041 ggcgtgacga cgaaattgcc agcacttcat ttccggcaat cgccgagcgg atcatcacaa
     2101 cgccaaaagc agacgctaaa ataactgcgc ctaacaaaag aaaatcgaaa tttctccagg
     2161 aaagtccacg tgtcataata aaaaacatgg atgaacccgt atcgctacag gttgatccat
     2221 gtcctattca aaaacatatc aacctgcgcg atgacgcgtt gcgcccttta atggaatatc
     2281 tgccaccaaa cgttgctcgc gtccatcttt ttccaattca attcgaacag cttcagggtc
     2341 aatctcaata tacttggaga ttgccttcaa gagttcatcc ttcaaagatt ctaactgccc
     2401 aggggttaag tccgtacggt cgtgaaccaa caccaactgt aatcgttctt ttgcgctcgt
     2461 agcgctccgc ttgcgtgtga agaagtccat atcacttcct tccagccaaa cgttgaatgg
     2521 tattccacaa gccactctgc ggagtaaggt ccataaatgg gacatcaact ccttgcaagc
     2581 gtttggcaat attacggaaa gcctgccccg cacggctctt tccatcggct gcaatcggag
     2641 tcccgcggtt cgaaccgatg attacatttt catcttctgg cacaatacca atcaaacgaa
     2701 tgcctaaaag atccaacaca tcttccgcag aaagcatgtc gttgtttttc acaagagcag
     2761 gattcagacg attgatgata agcgccgggt tgcctttttc ttccgcttca agaatgccta
     2821 ccactcggtc tgcatcgcgc accgcactga cttcggggtt ggtcactacg aggacgctgt
     2881 ctgctgcggc gatggcgttt cgaaatccgc gctcgatacc tgcaggggag tcaatgacga
     2941 tgaaatcagt atccggtctg aggtcattgc agatgcggat catgtcagat ggcgagacag
     3001 catttttgtc acgtgtttga gccgccggaa tcaggtacat atcctgataa tgcttatcgc
     3061 gaatcattgc ctgtttgagt ttgcaacgtc cttcgatgac atccacaata tcgtagacga
     3121 tacgattctc aagccccatc acaacatcaa ggttacgcaa cccaatatca ccgtcaatac
     3181 aaacgacccg tttgccatcc atggcaagag ccgtagcaag attggcaact gcagtggtct
     3241 ttcccacgcc gccttttcca gaagttacag tgatcacttg agctgtcata ttatttccta
     3301 attaaagttt cgccggtctg ccataattct gcttgcaatt taccttcgct gttaatgctg
     3361 gcaatctcgg gctttgggtc tttctgtggt ttcaacattg cggatgactc atttgcaatc
     3421 cgtaattggt ttgcagatag gtctagggca caaataaaag cggagcgatc tccttttacg
     3481 ccagcatgga tcactccacg cacacgcccc caaacgatca catttccttc tgccacgatc
     3541 tctgcgccgg ggttcacatc tccaaagacg acaatcgtac ccggatattc tatccgggtg
     3601 ccagaacgaa ctgtttttga aacgaaaagt gcagtgtcgc ttgatactgc ctcatg
//
LOCUS       DAOREF010000200         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15753_length_3654_cov_4.090581, whole genome shotgun sequence.
ACCESSION   DAOREF010000200 DAOREF010000000
VERSION     DAOREF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15753_length_3654_cov_4.090581"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKI33_05465"
     CDS             <1..276
                     /locus_tag="PKI33_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNM36487.1"
                     /translation="AIQALRDYAAKPSAANLPSTPKPIIEVKDEADRPQPRLDRLTGK
                     GMTTVVGRVRRDPILDLKFVVLSHNTIRGAAGASIYNAELLVNEGLL"
     regulatory      391..509
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            623..1909
                     /locus_tag="PKI33_05470"
     CDS             623..1909
                     /locus_tag="PKI33_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNM36488.1"
                     /translation="MSTDRKLGFATRQLHAGYSPDATTGSRAVPLYQTTSYQFKSTEH
                     AANLFALKELGNIYTRLMNPTTDVLEQRIASLEGGVAALASSSGHAAQAQAIFTLCNA
                     GDHIVTSSRLYGGTYSQFKYTLPKLGIDVTFVDPSDPKNFEAAIQPNTKIIYGETLGN
                     PDISVFPFEEVAEIAKKHNLILFIDNTFATPYLFRPFEWGAHVITHSTTKFITGNGTS
                     IGGIIVDGGNYDWTSEKFPNFNTPDPSYHGLVYSSIAAAGLPPFAIKARVHVLRDIGA
                     SASPFNSWQTLLGIETLNLRMERHTQNAQAVAEFLEKHPKVSWVKYPGLKSHPDNDRA
                     KKYFPKGVGAMMGFGVKGGLEAGKKFIESLQLFSHVANVGDARSLAIHPGSTTHSQLT
                     EDQQITAGVSPDFVRLSIGLEDIDDILWDLDQALNS"
     gene            1990..2925
                     /gene="metA"
                     /locus_tag="PKI33_05475"
     CDS             1990..2925
                     /gene="metA"
                     /locus_tag="PKI33_05475"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNM36489.1"
                     /translation="MPVKIPSTLPAKTTLENENIFIMDEDRALHQDIRALRVAILNLM
                     PTKISTETQILRLLSNSALQVEITLLNTATHESKNTDADHLLEHYVTFEDIKHEKFDG
                     LIITGAPVEQMPFEEVDYWQELKQIMDWSETNVESTFHICWGAQAGLYHKYGIPKYDL
                     PHKMFGVFEHRLLNRTESLMRGFDDIFLAPHSRHTEIRRADIEKHSDLQILAESEDAG
                     VYIVSDRIGRNLYITGHSEYDPFTLKAEYDRDVNKGLLIHVPKNYYPNDDPTQTPNVR
                     WRGHANLLYVNWLNYYVYQSTPYDLNQIPQKSVKN"
     gene            2990..>3654
                     /locus_tag="PKI33_05480"
     CDS             2990..>3654
                     /locus_tag="PKI33_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNM36490.1"
                     /translation="MNTPRKFGFNTRQLHEGYEPDPATGSRAVPIYQTTAYDLQSTDR
                     AARLFALQENGNIYTRISNPTSNVFEKRIASLEGGIGALEVGSGHAAQATAILTICEA
                     GDHIVSTSTLYGGTVTQFKYTFPKLGIHVTLVDPTDPENFRRAIQPNTKIIYGETISN
                     PLGNIFPFDEVSAIAHEHGIPLMIDNTFATPYLCRPFEWGADIVTHSTTKFIGGHGTT
                     MGGV"
BASE COUNT          959 a         1022 c          839 g          834 t
ORIGIN      
        1 gcgattcaag cattacgcga ttacgcggca aaaccgtcag cggcgaattt gccttcgacg
       61 cccaaaccaa ttatcgaggt taaggatgag gcggatcgtc ctcagccaag gcttgatcga
      121 ttaacaggca aaggtatgac gacggtagta ggccgcgtga ggcgcgatcc aatccttgat
      181 cttaaatttg ttgtgctttc tcataacaca attcgcggcg cggcgggcgc atcgatttac
      241 aatgcggagt tgttggtgaa tgaaggattg ctttagaacg atgatatacg atggaacaca
      301 accatcctat atcatccgtg gttcattgcg aatttcacac ttgacaatca cagactcgct
      361 atgatataac cgcgcgaaat taaatattcg ctcttatcca gagagactga gggaccgacc
      421 ctttgacgtc tcggcaacca aacgaaagtt caggtgccaa attcggcaaa tacgaatgac
      481 aagagtcatt catatctgga agatgagagg acggtgtaga cgtatacctc ttcttgtact
      541 ttcagaagag gtgatttttt taaatttaaa aattatctct tctgacgggc ggataaacat
      601 tattcaagag gagaacactc aaatgtccac agatcgaaaa ttaggttttg ccacccgcca
      661 gcttcatgcg ggctactcac cggatgccac cacaggcagc cgcgctgtac cgttgtatca
      721 gaccaccagc tatcaattca agagcacaga acatgctgcg aatctgttcg cgttgaaaga
      781 gttgggaaat atttatacgc gtctgatgaa cccgaccacc gatgtgctcg aacagcgcat
      841 tgcttcgctc gaaggcggcg tggcggctct cgcttccagc tcgggacatg cggcgcaagc
      901 gcaggcgatc ttcacgctgt gcaatgcagg tgaccacatt gtgacctctt cccgtttgta
      961 tggcgggact tacagccagt tcaagtacac cctgccaaag ttgggcattg atgtgacttt
     1021 tgttgacccc agcgacccga agaattttga agcggcaatc caaccgaaca ccaagatcat
     1081 ctacggcgag actctcggca accctgatat ttccgtcttc ccgttcgaag aagttgcaga
     1141 gatcgcgaag aaacacaatt tgattttgtt catcgacaat accttcgcca ctccgtatct
     1201 gttccgcccg tttgaatggg gcgcgcatgt tatcacccac tcgaccacca agttcatcac
     1261 cggcaatggc acatccattg gcggcatcat cgtggacggt ggcaactacg actggaccag
     1321 cgagaagttc ccgaacttca acacacccga tccttcgtat catggattgg tgtactcctc
     1381 catcgccgcg gctggactac ctccctttgc catcaaagcc cgcgtgcatg tgctgcgtga
     1441 tatcggcgct tctgcttcgc ccttcaactc atggcagacc ttgctcggta tcgagacact
     1501 caacctgcgc atggaacgtc acacccagaa cgcgcaagcc gtggcggaat ttttggagaa
     1561 acacccgaag gttagctggg tcaagtatcc tggactcaag agccatcccg ataatgaccg
     1621 cgctaaaaaa tacttcccca agggtgttgg tgcaatgatg ggttttggcg tgaaaggcgg
     1681 tttggaagca ggcaaaaaat tcatcgaaag cctgcaactg ttcagccatg ttgccaatgt
     1741 cggtgatgcg cgcagccttg ccattcaccc cggctccacc acgcacagcc agttgaccga
     1801 agatcagcag atcactgcgg gcgtcagccc cgatttcgtt cgtctctcca taggtcttga
     1861 agatatcgac gatattcttt gggatctgga tcaagcctta aattcttaag gatgaattat
     1921 gaaggatgag ggatgaatgt gatgaattta tttcatcctt catccttccg cctttatcct
     1981 tgaaaaacca tgcccgtaaa aataccttcc accctccccg ccaaaacgac tctcgaaaac
     2041 gagaacatct tcatcatgga tgaagaccgc gccttgcatc aagatatccg cgccttgcgc
     2101 gttgccatct tgaatctgat gccaacaaaa atttccaccg agacacagat cctgcgcttg
     2161 ctttccaact ccgcgttgca agtggagatc accctgctca acacagcaac gcacgaatca
     2221 aaaaatactg acgcagacca cctgctcgaa cattatgtta ctttcgaaga tataaagcat
     2281 gaaaagttcg atggtctcat catcaccggc gcgcccgtgg agcagatgcc ttttgaagaa
     2341 gtggattact ggcaggaact caagcagatc atggattggt cggaaacaaa cgtcgaatcc
     2401 acctttcaca tttgctgggg cgcgcaggcg ggcttgtatc acaaatatgg gattccgaaa
     2461 tatgacctgc ctcacaagat gtttggcgtc tttgagcacc gcctcttaaa ccggaccgaa
     2521 agcctgatgc gcggcttcga tgacattttc ctcgctccac actcccgcca caccgagatc
     2581 cgccgcgcag atattgaaaa gcattctgac ttgcaaatcc tcgctgagtc agaggatgca
     2641 ggtgtgtaca tcgtcagtga caggattggg cgcaacctct acatcaccgg gcattccgag
     2701 tatgacccgt tcacactcaa agccgaatat gaccgcgacg tgaacaaggg acttctcatt
     2761 cacgtgccga aaaattatta tcccaatgac gacccaacgc agactcccaa cgtccgctgg
     2821 cgtggacatg ccaatttgtt atatgtgaac tggttgaatt actacgtcta tcaatctaca
     2881 ccgtacgacc tgaatcagat tccgcagaag tcagtgaaga attagacgat ggacggtaga
     2941 cgatagacca tcgtcaatcg tccgtggtcg atcgtcaaaa aaggaagaca tgaacacacc
     3001 ccgcaaattc ggtttcaaca cccgtcaact gcacgagggc tacgagcctg accccgccac
     3061 aggcagccgc gctgttccca tttaccaaac caccgcctac gatctgcaaa gcactgaccg
     3121 cgctgcacgt ctcttcgcct tgcaagagaa cggcaacatt tatacgcgta tctccaaccc
     3181 aaccagtaac gtcttcgaaa aaagaatcgc cagcctcgaa ggcggaatcg gcgcgctgga
     3241 agttggttca ggtcacgccg cgcaagccac tgctattctc accatttgcg aagcgggcga
     3301 tcacatcgtt tccacttcca cactgtatgg cggcacggtc acacaattca aatatacatt
     3361 tcccaagctt ggcattcatg tcacacttgt tgaccccact gacccagaaa attttcgccg
     3421 cgccattcag ccgaacacaa agatcatcta cggcgaaacc atcagcaatc cgctcggcaa
     3481 catcttccca tttgacgaag tttccgccat cgcgcacgaa catggcatcc cgctgatgat
     3541 tgacaacacc ttcgccacgc catacttgtg tcgtcccttc gaatggggcg cggatatcgt
     3601 cactcactcc accaccaaat tcatcggcgg acacggcacc accatgggcg gagt
//
LOCUS       DAOREF010000201         3653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15763_length_3653_cov_4.120345, whole genome shotgun sequence.
ACCESSION   DAOREF010000201 DAOREF010000000
VERSION     DAOREF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15763_length_3653_cov_4.120345"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /locus_tag="PKI33_05485"
     CDS             complement(<1..2134)
                     /locus_tag="PKI33_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNM36491.1"
                     /translation="MSNFSNPSSPLKQTGQSETPNRKFSLGFKLPVAFIGLLLVALAL
                     YAFLSIRLSQQALVNTLRGELQDQVDTQVEVLQSNLLVAKAIASQLAAFAETEDFNES
                     DVPLLLQNTLKRNEHIYGSTIAYEPNAFRENLEYYAPYYYRSPDNVLEYADLGTPEYD
                     YFRKDWYTLPKAALTPIVTKPYFDYGGGNIWMVTWSIPFFSKNGEFKGVATADIAFLQ
                     TQEIVNKIKVGDNGYAFLIDSGGTILGIGENGGEYRVMSDSMVLAAYTEKTFRWIELV
                     SDMRAGKSGFIEATDPRGEPMLVAFAPIGLDTGWSLALAYPKSELTNTTAQLRNTLLG
                     YSIAVLALFSVLLFIFTRSITEPLRKLTAFASRVSGKTMSSNELNFHEQIHIKTQDEL
                     EDLGNAFNQMSEELRTSFGMLEENVESRTRDMARLANELRTIAEVNSEIAVIRDQNTL
                     LNVSANLIRERLGFYHVGIFLIDERSEYAALRGASSNAAEKMLASNLRIKVGEVGAVG
                     TVTITGQAYIAQDVDIDALQTSNQYLPETRSEISLPLRSYNVTIGVLDIQSKVQKAFD
                     GQDIQSLQVLADQLAAAIENAQLVQRLEQALSELSKNNLLKTQEIWHSAARRLSNSAY
                     EYDGLQIRPIPQNISPELVSELESGHPIINNDKGKNTLFVPLTVLGQVIGVIGLEQED
                     PSKQWSNEQIAIAQAAANRAALTLENARL"
     gene            complement(2131..>3653)
                     /locus_tag="PKI33_05490"
     CDS             complement(2131..>3653)
                     /locus_tag="PKI33_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36492.1"
                     /translation="LILAIRGNFKYLFIFPLVTSITLCIVSITDGKGSHDLIWVGPMG
                     LFLLANVYTRKNSIGPILLGLLNIALFVATGIAEIYKVIPNQYGTNTEHILLGVLYLS
                     SIMGAITGIFHRHRNLLRIAVDSKNEEEQVKKQYEEVISTLDQQVNARTAELDAINQE
                     LLSKTARLQAASDISQELTKIFYEHSVDLLSYTTRLISEKLGYYHVGIFIVDKSRGFA
                     VLQAANSKGGQQMLTKHHQLKVGGTGIVGYVAQSGRPRIALDTGADAVFFNNPYLPET
                     RSEISLPIKVLNNVIGVLDVQSTQSAAFTNEDTNILMTIANQLALLLFEEEGSTSTVS
                     TIRKRTQFSDLERQFGYSFRTDGSVVANTTIDPSPSIKRAIASGETVTINSASKDGLP
                     MLAVPVKFREQVIGIIQVEASEKNRNWSEDEVLLVQAISDRAALALENARLFENATRR
                     AEQEETISRVTTQIGSSTDFDRIMQTTIQELGVALGASRSFIQIGSLSNSDEKASE"
BASE COUNT         1012 a          770 c          785 g         1086 t
ORIGIN      
        1 aaaggcgggc attttcaagt gtaagcgcag cacgattggc tgcagcttgt gcaatagcga
       61 tttgttcatt tgaccattgt ttagatggat cttcctgctc caaaccaatg actccaatga
      121 cttgtccaag tacggttagc ggaacaaata gagtgttttt tcctttatca ttattgatga
      181 ttggatgtcc gctttctaat tcagaaacca actcaggcga gatattttgt ggaatggggc
      241 gaatttgcaa gccatcgtat tcgtatgcag agttacttaa ccgtctagcc gccgagtgcc
      301 aaatttcttg agtttttaat aggttgtttt ttgaaagttc agaaagcgcc tgttcaaggc
      361 gttgaaccaa ctgcgcattt tcaatggcag cagcaagttg atcggcaagc acttgtaaac
      421 tttgaatgtc ttgtccatca aacgctttct gtacttttga ttgaatatcc agcacgccaa
      481 tggttacatt gtagctgcgt aatggaagcg agatttcaga ccgtgtttct ggtaaatact
      541 gattactagt ttgcaaagcg tctatgtcta catcttgtgc aatatacgct tgtcctgtaa
      601 tcgtaactgt tcctacggct cctacttcgc caactttaat tcttaaattg cttgcaagca
      661 ttttttctgc tgcattgcta cttgcaccac gcaaagcggc atattcgctt ctttcatcaa
      721 taaggaagat accaacatga taaaagccca gacgttctcg aattaagttc gcagacacgt
      781 tgagaagtgt attttggtcg cgaattaccg ctatttcgct gttaacttct gcaatagtgc
      841 gaagctcatt tgctaagcgc gccatgtctc tcgtacgtga ttcaacattc tcttccaaca
      901 taccaaacga tgtacgcaac tcttcagaca tttggttgaa tgcatttcca aggtcttcca
      961 actcatcctg tgttttgata tggatttgtt catggaaatt tagctcgttg ctgctcatgg
     1021 ttttcccaga tacgcgactt gcaaaagcag ttaacttgcg aagtggttct gtaatagaac
     1081 gtgtgaaaat aaatagtaaa acgctaaaga gtgctaatac agcaattgaa tatccaagca
     1141 aagtatttcg gagttgagcc gtggtgtttg ttaactctga ttttggatac gcaagggcta
     1201 aggaccagcc agtgtctaac ccaattggag caaaggcaac taacatcggt tcaccgcgcg
     1261 ggtcagttgc ttcaataaaa ccgcttttgc ctgctcgcat atcagaaact aactcaatcc
     1321 agcggaaggt tttttcggtg taggcagcca ataccattga gtcagacatg actctgtatt
     1381 caccaccatt ttccccaatc ccaagaatag ttccaccaga gtcgattaag aatgcataac
     1441 cattatcgcc aacttttatc ttgttgacga tttcttgcgt ttgaagaaat gcaatatcag
     1501 cggtagctac acctttgaac tcaccgttct ttgaaaagaa cgggatactc catgtcacca
     1561 tccagatatt tccgccgcca taatcgaaat agggtttggt tacgattggg gtaagtgcag
     1621 cttttggcaa ggtgtaccaa tcttttcgga agtaatcgta ctctggtgtt cctagatcag
     1681 catattctag aacattatcg ggcgagcgat aatagtaagg agcataatat tccaggtttt
     1741 ctctaaacgc attcggctca taagcaattg ttgacccata tatgtgctcg ttgcgtttca
     1801 aggtgttttg caaaagcaaa gggacatcac tctcgttaaa atcttctgtt tctgcaaatg
     1861 ctgccaattg agaagcaatc gcttttgcaa ctaatagatt agactgaagc acttctactt
     1921 gagtgtcaac ttggtcttga agttcgccgc gcaatgtgtt aactagtgct tgttgtgaaa
     1981 gccgaatgct taagaaggca tacaacgcca atgcgaccaa taacaaacca ataaatgcga
     2041 ctggcaactt aaaccctaaa ctaaattttc gattgggcgt ttcactttgc cccgtctgtt
     2101 tcagtgggga ggagggattg gagaaattgc tcattctgac gccttctcat ctgaattcga
     2161 aagagaacct atctgaataa acgaccgaga tgccccaagg gcaacaccaa gttcttgaat
     2221 ggtcgtttgc atgatgcggt caaaatcagt tgaagaaccg atttgggttg taacacggga
     2281 gattgtttct tcttgttctg ctctgcgggt ggcattctca aacagacggg cattttccaa
     2341 tgccagggca gcacggtctg aaatggcttg caccaaaaga acttcatctt cgctccagtt
     2401 tctatttttc tctgaagctt caacttgaat gatgccaatc acttgttcgc ggaacttaac
     2461 aggaacagcc aacatgggca acccatcttt tgaagcgctg ttaatggtaa cagtctcgcc
     2521 tgaggcgata gctcgcttaa tagacgggct ggggtcaatt gttgtgtttg cgaccacact
     2581 tccatcggtg cggaaggaat atccgaactg tctctcaagg tctgagaatt gagtacgctt
     2641 tctgatagta gagactgtag atgttgagcc ttcttcttca aatagaagta gtgctaattg
     2701 attagcaatg gtcataagta tgttcgtatc ttcattcgta aatgccgcag actgtgtgct
     2761 ttgcacgtct aatacaccaa taacattgtt caaaaccttg ataggtagtg atatttcaga
     2821 acgtgtttct ggcaggtaag ggttgttgaa gaagaccgca tctgcccctg tatcaagagc
     2881 gatgcgtgga cgcccactct gcgcgacata acctacaatg ccggttccgc ctactttgag
     2941 ttgatgatgt tttgtgagca tttgttgccc acctttgctg ttggctgctt gaagaacagc
     3001 aaacccgcga gacttatcaa caataaaaat cccaacatga taatagccaa gcttctcact
     3061 aatcaatcgt gtagtgtagg aaagcaaatc tacactgtgt tcgtagaaga ttttggtcaa
     3121 ctcttgcgaa atatctgatg cagcctgcaa gcgtgcggtc ttgcttagta attcctggtt
     3181 aatcgcatct agctcagcag tacgtgcatt cacttgttga tcaagagtgg aaatgacttc
     3241 ttcatattgt ttcttgacct gttcttcttc atttttacta tcaacagcaa ttctaaggag
     3301 attgcgatga cggtggaaaa tacctgtgat tgcacccatg atagaggata aatacaagac
     3361 acccagaaga atatgctctg tattagtgcc gtattggtta ggtatgactt tatatatttc
     3421 tgctatacca gttgctacaa ataaggcaat atttaataag ccaagaagaa taggacctat
     3481 tgaattttta cgcgtgtaaa cattggctag caagaatagc cccattggac caacccaaat
     3541 cagatcgtga gaacctttcc cgtctgtgat ggatacaatg caaagagtaa tagaagttac
     3601 aagtggaaat ataaatagat acttgaaatt gccgcgaata gctagaatga gag
//
LOCUS       DAOREF010000202         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15780_length_3651_cov_4.208843, whole genome shotgun sequence.
ACCESSION   DAOREF010000202 DAOREF010000000
VERSION     DAOREF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15780_length_3651_cov_4.208843"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKI33_05495"
     CDS             <1..196
                     /locus_tag="PKI33_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNM36493.1"
                     /translation="ELPPDLCKEIEKHLEGCDNCRIVLNTTRRTIDLVQIPVEENVPD
                     DVRERLFKRLNLDDYLKQQS"
     gene            218..1399
                     /locus_tag="PKI33_05500"
     CDS             218..1399
                     /locus_tag="PKI33_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNM36494.1"
                     /translation="MKTIQNFAFLASLLLISACQTLPVETPAISPTTLAETVVPPNTP
                     APSPTPNFTPTVPAAVIELQGTTLPSGFSILKFAEIYRPTSLTVDLNGNILVASADSN
                     IYIVSDADKDGHADTQSIFASGYYIPLGLTVHPATGDVYVSYQGAIAVLTDSDKNGEA
                     DSSRILVEGLPSTGRHQNNNLEFGPDGWLYMGQGSTCDACAEADARNATIMRFNLETG
                     EKEIFASGLRNPFDIAFHPLTGELFSTDNGRDDLGLDMPNEELNLILQNGSYGFPNCW
                     NEQNTNGCENTIPAVAFFEPHSSADGLDFYKGSQFPIEYQNNAFVGIFGSWLNPTLET
                     GIKRVILTPKDGIYTSEVSWFAKFPKGVMPLPLQFGPDGALYVGDYINDVVYRISYGV
                     P"
     gene            complement(1434..1504)
                     /locus_tag="PKI33_05505"
     tRNA            complement(1434..1504)
                     /locus_tag="PKI33_05505"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1470..1472),aa:Gln,seq:ctg)
     gene            complement(1554..1892)
                     /locus_tag="PKI33_05510"
     CDS             complement(1554..1892)
                     /locus_tag="PKI33_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36495.1"
                     /translation="MSQSDQNKTNFAGTYFDKDAVIRIAKLLKLASWIVAGVYFFQFV
                     VNVGIFTLQILRKLLYFGGPTDFMQQLLWQVQPSLAGLIFFASLQAIALALLILLDIE
                     DNTRRAARGK"
     gene            2049..>3651
                     /locus_tag="PKI33_05515"
     CDS             2049..>3651
                     /locus_tag="PKI33_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNM36496.1"
                     /translation="MSPEENSKPSDFIREAVAEDLKSGRFNYVRTRLPPEPNGYLHIG
                     HVKAFMIDYLVAKENGGELVLRFDDTNPAKEETEFVQAIEEDARWLGIEWVKVTYASD
                     YFDELYDWAKKLVLMGKAYVDDQTPEEVSKNRGTLTQPGVNSPYRDRDVEENLDLLER
                     MKNGEFPDGTRILRAKIDMAHPNINMRDPAMYRIIHTPPHHRTGDKWKIYPMYDWSHG
                     QNDSMEGITHSLCSLEYENHRPLYEWFLEQLGVFKPRQIEFARLNMNYTVMSKRKLRR
                     LVEEKLVSGWDDPRMPTLRGMRRRGYTAEAVLDFIRRVGVAKNYSVSDVALLEECVRD
                     DLNKHALRRMAVIRPLKVVIDNYPDDLVEEMSAINNPEDAEAGTRKVPFSKVLYIEQD
                     DFREVPPAKYYRLFPGNEVRLRYAYFIKCTHVVKNDAGEVIEVHATYDPSTKGGDSPD
                     GRKVKSTIHWVSAKHAKEVEVRMYDRLFNKENPEEGEEGFLNCLNPNSLEILHGYVEP
                     ALASAETGSRFQFERLGYFCVDKEST"
BASE COUNT         1039 a          839 c          837 g          936 t
ORIGIN      
        1 tgaactaccg cccgatttgt gcaaagaaat cgagaagcac cttgaaggct gcgataactg
       61 tcgcatcgta ctcaacacca ctcgccgcac tattgacctt gtacaaattc ctgtggaaga
      121 aaacgtacca gatgatgtaa gagaaagatt attcaagcgg ctaaatctgg atgattactt
      181 aaagcaacaa agttaattgc cactataatt tgatgccatg aaaacaattc aaaactttgc
      241 ttttctggca tctttgcttt taatctcagc atgccaaact ttacccgttg aaactccggc
      301 tatctccccc acaactcttg ctgaaacagt tgttccacca aatacccctg ctccatcgcc
      361 tacacctaac ttcacaccga ccgtcccagc cgctgtgatc gaacttcagg gaacaactct
      421 acccagcgga ttctcaattc ttaagtttgc agaaatttac cgtcccacct cgttgacagt
      481 agacctcaat ggaaacatcc tcgtcgcttc ggcagatagc aatatttata tcgtatcaga
      541 tgcagataaa gatggtcatg ccgatacgca atctattttt gcatccggat actatattcc
      601 gctcgggttg accgtgcacc ccgcaacagg ggatgtatat gtctcatatc aaggcgcaat
      661 tgcagtacta acagattcag acaaaaatgg agaggcggat tccagccgca ttttggtgga
      721 aggtttaccg tctacagggc ggcatcaaaa taacaatctt gaatttggtc cagatggctg
      781 gttatatatg ggacagggct ccacttgcga tgcctgtgcc gaagccgatg cgagaaatgc
      841 aacaattatg cgtttcaacc tagaaacagg cgaaaaagaa atctttgctt ctgggctgcg
      901 caatccgttt gatatcgctt ttcacccgct tacaggcgaa ttgttttcca ctgataatgg
      961 gcgtgatgac cttggtttag atatgccaaa cgaagaacta aaccttattc ttcaaaatgg
     1021 gtcctatggc tttccaaatt gttggaatga acaaaacacc aatggttgtg aaaacacaat
     1081 tcctgctgtg gcattttttg agccgcattc ttcagcagat ggtttggatt tttacaaagg
     1141 aagtcaattt ccgatcgaat atcagaataa tgcctttgta ggaattttcg gctcttggct
     1201 aaacccgaca cttgaaaccg gtatcaagcg agtgatcctt acgccgaaag atggtatata
     1261 taccagtgaa gtctcttggt ttgccaaatt ccctaaagga gtcatgccct taccactcca
     1321 atttggtcct gatggtgcat tgtacgttgg ggattacatc aatgacgtag tgtatcgcat
     1381 cagttacgga gtgccgtaaa taaaaaaact ccctaaacca agggagtttt gtgctgggga
     1441 tcaaggattc gaacctcaaa tttcgcgtcc agagcgcgac gtgatgccat ttcaccaatc
     1501 cccaattttc cagcgggcgg tattttatca cgcaccctca gagagggcaa gccctatttt
     1561 ccgcgtgcgg cgcgacgggt gttatcttca atgtcgagaa gaatgagaag cgccagtgca
     1621 atcgcttgta aagaggcaaa aaatatcaac cctgccaaac tcggttgaac ttgccagagt
     1681 agttgttgca taaaatcggt aggtccgcca aagtaaagaa gtttgcggag tatttgcaaa
     1741 gtgaaaatac ctacattcac tacaaattga aaaaaataca ctcctgctac aatccatgaa
     1801 gcgagtttaa ggagctttgc aatgcggatg acagcatctt tgtcaaagta ggttccggca
     1861 aaattggtct tattttggtc agattgagac atacattcct ccagagaata gttattatac
     1921 tttgttataa ttagcacgaa tacgacgctc ccagcgggga caaggttcct gaaaggctgg
     1981 caggtgtcgg ctagaaaaga ggcggaaatt ccttccgcat aaatccatca tgaaggttaa
     2041 gtcaaagaat gagtcctgaa gaaaatagca aaccgtctga tttcatccgc gaagctgtgg
     2101 cagaagactt aaagagcggg cgcttcaact acgtccgtac gcgcctgcct cctgagccaa
     2161 atgggtactt acacattggg catgtcaagg cgttcatgat cgattatttg gttgccaagg
     2221 aaaacggcgg cgagttggtt ttgcgttttg acgataccaa ccctgccaaa gaagagactg
     2281 aatttgtgca agccatcgaa gaggatgcgc gttggcttgg gattgaatgg gtaaaagtaa
     2341 catacgcatc agattatttt gatgagcttt acgactgggc aaagaaactg gttttgatgg
     2401 gcaaagctta tgtagatgac cagaccccag aggaagtaag caagaatcgc ggcacgctca
     2461 cgcaacccgg cgtaaactct ccctatcgcg accgcgatgt agaagaaaac ttagacttgc
     2521 tcgaacgcat gaagaacggc gaattccctg acgggactcg tattttacgc gccaagattg
     2581 atatggcgca tccgaatatc aacatgcgtg acccggcaat gtatcgcatc atccacaccc
     2641 ccccgcatca ccgtaccggt gacaagtgga agatttaccc aatgtatgat tggtctcatg
     2701 ggcagaatga ttcgatggaa ggtatcactc attcgttatg ttcgcttgag tatgagaatc
     2761 atcgtccatt atatgaatgg ttcttagaac agcttggcgt tttcaaacca cgtcagatcg
     2821 agtttgcgcg tttgaacatg aactatacag tgatgagcaa gcgcaaactt cgccgccttg
     2881 tcgaagaaaa actggtcagc ggttgggatg acccccgtat gccaacattg cgcggtatgc
     2941 gcaggcgcgg ctacaccgct gaagcggttt tggatttcat tcgccgtgtg ggtgtggcaa
     3001 aaaattatag cgttagtgat gtggcactac tcgaagaatg tgtacgtgat gatctcaaca
     3061 aacatgcttt gcgtcgcatg gcggttatcc gcccgctgaa agtagtcatc gataattacc
     3121 ctgacgacct cgtcgaagag atgagcgcca tcaacaaccc tgaagacgct gaagccggca
     3181 cccgcaaagt gcctttctct aaagtccttt acattgaaca ggatgatttc cgtgaagttc
     3241 cgcccgcgaa atattaccgt ctcttccccg gtaatgaagt ccgcttgcga tatgcatatt
     3301 tcatcaaatg cacccatgta gtgaaaaatg atgctggtga agtgatcgaa gtccatgcca
     3361 cttatgaccc atccacaaag ggtggcgatt cacctgatgg gcgaaaagtg aaatctacga
     3421 tccattgggt ttctgctaaa catgcaaaag aagtggaagt ccgcatgtat gatcgtctct
     3481 tcaacaaaga aaatccagaa gaaggtgaag aaggattcct aaactgtttg aacccgaact
     3541 ctttggaaat tcttcatgga tatgtagagc cggctcttgc ctccgccgaa actggttcac
     3601 gtttccaatt tgaacgtctc ggatatttct gtgtagacaa agaatcgact t
//
LOCUS       DAOREF010000203         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15793_length_3650_cov_2.662031, whole genome shotgun sequence.
ACCESSION   DAOREF010000203 DAOREF010000000
VERSION     DAOREF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15793_length_3650_cov_2.662031"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKI33_05520"
     CDS             complement(<1..405)
                     /locus_tag="PKI33_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNM36497.1"
                     /translation="MKILTVVGARPQFVKAAAVSRILRETHTEVLVHTGQHYDERMSE
                     VFFRELDIPEPGYNLEVGSAGHSVQTGEMLIRMEPIFEKEKPDWVLVYGDTNSTLAGA
                     LVASKLHIPIAHVEAGLRSFNREMPEEINRVLT"
     gene            complement(532..1617)
                     /locus_tag="PKI33_05525"
     CDS             complement(532..1617)
                     /locus_tag="PKI33_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM36498.1"
                     /translation="MRIAYVTVHVAPEIMQGGVGKKIRTQMKIWRGLGHEVTLFSLTP
                     AEIPFPDERQFIFDAKTNLLKRESNRALGLMSMLDAIRKYKPDIIYLRFGLYSYPMHR
                     LFKIAPVILETNSDDRQEYQKRGTFFYWMNRLTRNLTFGSASGIITPSHELVDILLPK
                     RDKPFRVISNGIDLSQAEMLPPTNNRQPVITMVCSPGMKWHGVDKLMQFAEKTPDVFV
                     HLVGYTEKDMGVPVPANVRLHGFLDKQGVRTVLAQSDVALGTLALHRNQMQEASVLKV
                     REALAYGIPVVIAFHDTDLHNVELETILCIPNTEQNVSENAERIRKFAYDMLGKRVDI
                     NSVAPYLDQRKKEEIRLAFFEQTLQSL"
     gene            1808..2404
                     /locus_tag="PKI33_05530"
     CDS             1808..2404
                     /locus_tag="PKI33_05530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36499.1"
                     /translation="MFAEEINLILNASEYSGGAVLETGCGNGALFPYLNINKEDYTGV
                     DLSDSLLEIFQNNHPGVNVISGDASSYNSDRRFSLIFSNGVIQYFDKKMLEKYIQNST
                     EMLEQDGILLLGNLLWKDVRNQYYSGELVTGENIQKPKYPRLKLLKAKLWSLLGKNDF
                     MGHSYNPRDFFHYNSDVTIYGSLFHPYRFSVVIKKKLS"
     gene            2405..3331
                     /locus_tag="PKI33_05535"
     CDS             2405..3331
                     /locus_tag="PKI33_05535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36500.1"
                     /translation="MYNSLFQKEWWLEAVAPGQWDVVVVKKGDELVARLPFVIEKKYG
                     FTILTQPPLTPMLGPWLRATNAKHSKQLSTQKELIWELLKKLPRHDLFSQSLHPSITN
                     WLPFYWEGFEQTTRYTYLLNELSNLDVLWDGFNEQVRRNIRKAQSKVTVKMGLDVERF
                     LDINEKTFLRQQRKLPYSRELVKRIDSAARDQNASQIFYAEDYTGRIHAAIYIIWDEQ
                     SAYYMMGGEDPELRNSEASSLLMWRAIQFASNVTKVFDFEGSMIQPIERFFRGFGTKQ
                     VTYSHITRASKKLKVLQHMREIFRSITERNES"
BASE COUNT         1045 a          820 c          804 g          981 t
ORIGIN      
        1 cgttaatacg cggttgatct cctcaggcat ctcgcggtta aaactacgca atcccgcctc
       61 tacatgtgca atggggatat gtaatttgga agcgaccaaa gcacctgcaa gcgtcgagtt
      121 ggtgtcgcca taaaccagca cccaatcggg tttttctttc tcaaagatcg gttccattcg
      181 aatgagcatt tcacccgtct gcacggaatg ccctgctgag ccgacttcga ggttgtagcc
      241 cggctcaggg atatccaatt cgcggaagaa gacttccgac atgcgctcat cgtaatgttg
      301 cccggtatgt accagcactt cagtatgtgt ttcacgcaag atgcggctga cagcagcagc
      361 cttcacgaat tgagggcgtg ccccaaccac tgtaagtatt ttcatgatgg atcttttcca
      421 tatattcaaa ttgaagaaca aggggattat aactttaatg tcatggcgaa cggtaaagct
      481 acaaaaggtt agagatctgt atcatcgctg tttcgcagcg agcgatatcg atcagagtga
      541 ttgcagggtc tgctcgaaga aagcaagccg tatttcttcc tttttgcgtt gatcaagata
      601 cggtgcaacc gagttaatat ccacccgttt cccaagcata tcatacgcaa acttacggat
      661 acgctccgca ttctcactta cattctgttc cgtattgggg atacacaaga tcgtttcgag
      721 ctcaacattg tgcaagtcag tatcgtggaa ggcgatcacc acagggatcc catacgccaa
      781 cgcctcgcgg actttcagca ccgaagcttc ctgcatttgg ttacgatgca gtgccaatgt
      841 gcccaatgcc acatctgatt gagccagcac agtccgcacg ccttgcttat ccaaaaagcc
      901 atgcaaacgc acattcgccg ggacgggcac tcccatatct ttttcagtgt agccaaccag
      961 atgcacaaaa acatccggcg tcttttcagc aaattgcatc aacttatcca caccatgcca
     1021 cttcataccc ggtgaacaga ccatagtgat gacaggttgt ctattatttg taggcggcag
     1081 catttctgcc tgacttaggt cgatgccgtt ggaaatgact cgaaatggtt tatcccgttt
     1141 ggggaggaga atgtccacca attcatgaga gggggtaata attccggaag cagagccaaa
     1201 tgtcagattg cgggtgaggc ggttcatcca ataaaagaat gtgccgcgtt tttgatattc
     1261 ctgccggtca tcggaatttg tttccaagat cactggcgcg atcttgaaaa gacgatgcat
     1321 gggataagaa tacaacccaa aacgtagata gatgatatcc ggcttgtact tacggattgc
     1381 atcaagcatg ctcatcaacc ccaatgcgcg gtttgattcg cgcttcagga ggttggtctt
     1441 tgcatcgaag ataaattgac gctcatcagg aaaaggaatt tcagctggcg tgagactaaa
     1501 caaagttact tcatgcccca gcccgcgcca gattttcatt tgagtacgga tcttcttgcc
     1561 aacgccgcct tgcatgatct caggcgcgac atggacagtt acataggcaa tgcgcatatt
     1621 ttgagttaga atcttttcgt caacaaagtg tttgaatttt agtataaatc atatttaaga
     1681 tatctgcagg aaagacttaa tacaaagact ggaaattaaa aaaatgactc agataaataa
     1741 atggaaggag ttttggaatt tacaaacctc tcctttgcat agttacaact cacctacctg
     1801 gtataagttg tttgctgaag agattaacct tattttaaat gcatccgaat attcaggcgg
     1861 cgccgtgctt gaaacaggct gcgggaacgg cgccttgttc ccatacttga atatcaacaa
     1921 ggaagattat accggggtag atctctccga ttcattactg gaaatatttc aaaataatca
     1981 tcccggggtg aatgttatca gcggtgacgc ttcctcctat aattccgata ggcgcttttc
     2041 gttgattttc agcaatggag tgattcaata cttcgataaa aaaatgttgg agaaatatat
     2101 ccaaaactcg accgaaatgt tggaacagga tggcattctt ctcttgggaa atctcctttg
     2161 gaaagatgta cgcaatcagt attactcagg tgaattggtg accggagaaa acattcaaaa
     2221 gccaaaatac ccacgcttga aactcttaaa agccaaactc tggtcgttgt taggaaaaaa
     2281 cgatttcatg ggtcattctt ataacccccg agatttcttt cactataaca gtgatgtcac
     2341 tatctacgga tcgctttttc atccttatcg ttttagtgtg gtcattaaaa agaaactgag
     2401 ctagatgtac aactcgcttt tccaaaaaga gtggtggtta gaagctgttg ctccgggtca
     2461 atgggatgtt gtagtcgtca aaaaagggga tgagcttgtc gcccgtcttc cttttgttat
     2521 cgaaaaaaaa tacgggttca ccatcctcac acaaccgcca ttaacaccca tgcttggtcc
     2581 atggcttcgg gctacaaatg caaagcattc aaaacaatta tctactcaaa aggaattgat
     2641 ctgggaattg cttaagaaac tcccccggca cgatcttttc tctcaaagcc tccacccttc
     2701 gatcacaaac tggcttccat tctattggga aggtttcgaa caaaccacgc gatacacata
     2761 cttattgaat gaactgtcaa acctggatgt cctttgggac gggttcaatg aacaagttcg
     2821 caggaacatt cgtaaagcgc aaagcaaagt aaccgtcaaa atgggcttgg atgttgaacg
     2881 gtttctggat attaatgaaa aaacctttct gcgtcagcaa aggaaactgc cttattccag
     2941 agaactcgtc aaacgaatcg attctgctgc acgcgatcag aatgcaagtc aaatcttcta
     3001 tgcagaagac tatacgggtc gaattcatgc tgccatctat attatttggg atgagcaatc
     3061 cgcttattac atgatgggag gcgaagatcc tgaattgcgg aacagcgaag caagcagtct
     3121 cttaatgtgg cgtgcaatac agtttgcttc aaatgtaact aaagttttcg attttgaggg
     3181 aagcatgata cagccaatcg aacgtttttt caggggattc ggcacaaaac aggttactta
     3241 ctcgcatatt accagagcca gtaagaagtt gaaagttttg cagcacatgc gcgaaatttt
     3301 taggagcatt acagaaagaa atgagagcta aaacaacata taccgtggtg ttgactcacg
     3361 acatcgacca tatatcgctt cgaaagtatc cagtattcag caatgtaacc ttggagtttt
     3421 tcaaaagggg cttatggatt aacctattga gaaccctgca aggcgactta ccttttattc
     3481 agtatcttga ctcgctgcga tggtgtcttg ttgtcgtttt acctagtatg caatttggca
     3541 gtcggcggag taactctgca tcgtaccgtt atcccgccag cacgtaacta aaactgtagt
     3601 gtgaggagcg aaggccagat gatttcgacc aaaaatggcg ctaccacccc
//
LOCUS       DAOREF010000204         3640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15867_length_3640_cov_3.752580, whole genome shotgun sequence.
ACCESSION   DAOREF010000204 DAOREF010000000
VERSION     DAOREF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15867_length_3640_cov_3.752580"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1682
                     /locus_tag="PKI33_05540"
     CDS             120..1682
                     /locus_tag="PKI33_05540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM36501.1"
                     /translation="MLPPNVLYSAILLAAGVGCLVVAVAIWLKRREAIGAKSLFVFLL
                     GLSWWDITYGIFWADIPGPNPYFWLDITLFGAFVVPTAFLIFALEYSNSQKWLGRPFI
                     IAAIIEPILISILLCTDRWHNLYFGGVRALNTTMLVEMGPAAWANVVYSYLLILIGVL
                     VLAVTLYRSRGVYRMQVTFILAATVTPWVIHILFLSIGDHALLPDADVTPFIFSMTAL
                     LIAYALIRYRLLDVVPIARSILIESMSEGVCVLDSQNRIVDINQTARKALSLEFFIGQ
                     PVEQAFKCWIDIVSHFFDQETAQTEVVIDDRYLDLRISPVYDKNQRLLGKLVIWRDIT
                     ELKNIQLKLQQLATIDELTLAFNRRHFMEIAEVQFSQALRQNQPFALALIDLDYFKGI
                     NDKYGHPAGDQVLAEFSRMFMENIRKFDVFGRLGGEEFALLMPNTDIHFAYHASERLR
                     FMITNTPIPINGGDVVVTFSLGVAMLQGAQDTLSAIFRRADQALYAAKNSGRNQVVIW
                     EQGLEAVAQKQA"
     misc_binding    1729..1979
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2049..>3640
                     /gene="ileS"
                     /locus_tag="PKI33_05545"
     CDS             2049..>3640
                     /gene="ileS"
                     /locus_tag="PKI33_05545"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNM36502.1"
                     /translation="MFKAVNPKLDVPSMEDNVLKMWTKQEIFKKTVDQRQGKPEYVFY
                     EGPPTANGRPGVHHVLARAFKDMFPRYKIMRGYHVSRRGGWDTHGLPVEIEVEKQLGF
                     TNKQQIEEYGVDKFNELCKKSVFNYIQEWEKLTGRIGFWVDLDTAYVTYTNDYIESVW
                     WILKNFWEKDLLFKGYKIVPYCPRCGTPLSDHEVALGYEDTADPSVFVRFPLVDKPDT
                     SFLVWTTTPWTLPANVAIAAHPDVDYVTVERDNNGTTEKLILAKSLLEKVFRGEEVKV
                     VDTFKGKKLKGMKYQPLFTFVPLDKPAHYVVLGDFVTTEDGTGLVHQAPAFGAEDMEM
                     SKQYDLPVLLTIQPDGGFIPEVTPWRGVFFKDADPQIIQNLEARGLMFRSEKYVHTYP
                     FCWRCHTPLLYYARDSWYIRTSAKKENLVNLNKTINWVPDHIKNGRFGNWLENNIDWS
                     LSRERYWGTPLPVWECENGECKHRECVGSVAELSEKAGRDLTELDLHRPHVDNVHWKC
                     VDCGGQMTRVKDLIDVWFDSGS"
BASE COUNT          882 a          819 c          933 g         1006 t
ORIGIN      
        1 accaaccgtg gggtaggtat gactgagccg tttgttcgct ttaattgccg acctgagatt
       61 acaatattta ccctaaatca gggatgatat tcaagttggg ggtattgtat aatttgagta
      121 tgcttccacc gaatgtattg tatagtgcaa ttttgttggc ggcaggtgtg gggtgtttgg
      181 tagttgccgt tgcaatttgg ctgaaacgcc gtgaagccat aggggcgaaa tcgctttttg
      241 tattcctatt agggctaagc tggtgggata taacctatgg catcttttgg gcagatatac
      301 ctggaccaaa cccatatttt tggttagata tcaccttgtt tggtgctttt gttgtgccga
      361 ccgcgttttt gatttttgca ttggaatact ctaattcgca aaagtggctg gggcgacctt
      421 ttattattgc agcgatcatc gaacccattt tgatttccat cctgctgtgt acagaccgct
      481 ggcataacct ttattttggc ggggtgcgtg ctttgaacac gaccatgctt gttgagatgg
      541 gaccagctgc ttgggcaaat gttgtctact cgtatcttct aattcttatt ggcgtgcttg
      601 tattggctgt aacgttgtat cgttcgcgcg gggtctatcg tatgcaggtg acgtttatct
      661 tggcagcgac tgtgacgccc tgggtaattc acatcctctt tctttccatt ggtgatcatg
      721 ctcttttacc cgacgccgat gtgacaccat ttattttctc aatgacagcg ttactcattg
      781 catatgctct tattcgttat cgtttattgg atgtcgttcc gattgctcgc agtattctga
      841 tcgagtctat gagcgaaggg gtttgcgtct tggattcaca gaaccgcatc gttgacatta
      901 atcagacagc gcggaaggcg ctctctttgg agttctttat tggtcagcca gttgaacaag
      961 cgttcaagtg ctggatagat attgtctctc attttttcga tcaagaaacg gcgcagacgg
     1021 aggttgtgat tgatgatcga tatcttgatc tacgtatttc gcctgtgtat gataaaaatc
     1081 aacgcctttt agggaagttg gttatctggc gtgatattac ggaattaaag aatatacagc
     1141 tgaaacttca acagcttgcc accatcgatg aattgacact ggcattcaat cgccggcatt
     1201 ttatggaaat tgccgaagtg cagtttagcc aagcacttcg gcaaaatcaa ccctttgcct
     1261 tggcgttgat cgatttggat tacttcaagg gaataaatga taagtatggg caccctgcag
     1321 gagaccaagt tttagctgag ttttcacgca tgttcatgga gaatatccgc aaattcgatg
     1381 tgtttgggcg ccttggcggg gaggaatttg ctctgcttat gccaaacacc gatatacatt
     1441 ttgcatatca tgcttctgaa cggctacgat ttatgattac caatacgccc attccaatca
     1501 atggcgggga tgttgtggtg acttttagcc taggcgttgc tatgttacaa ggcgcacaag
     1561 acacgctctc tgccattttc cgccgagcag accaggcgct ctacgccgcg aagaattctg
     1621 gcagaaatca ggtagtgata tgggagcaag ggttggaagc agtagcgcaa aaacaggctt
     1681 aaactcacgc cttgctcatt ccctgcggct tgtgtataat gccgctatct aaaagcgtgg
     1741 atgggaacga gtaaaccgtt caagacgggc agcgagtccg aggtcaggtg tgaagcggat
     1801 acgttcaaga cggttgaaaa tcctcccgga gctgtgaact gaaatcagaa aattctgaga
     1861 gtagggaatc cgaaattgtt cttcgttatc agaacagggc gatggttgtc gtccaatgag
     1921 tgttcatcta cggatgaaaa tcagagtggt accgcgtgag ccttgctctc gtctctgtta
     1981 tgagatgaga gttttttatt ttaatttcac cgtcattgcg tggatgatga cggaaacaag
     2041 gagtaagaat gttcaaagca gtcaacccaa aactcgatgt gccttcgatg gaagataacg
     2101 tcctcaaaat gtggacgaag caggagatct tcaagaagac agttgaccaa cgacagggca
     2161 aacctgagta tgtcttttat gaaggtccgc ccaccgcgaa tggacgcccc ggtgtacatc
     2221 atgtgctcgc gcgtgcgttc aaagatatgt tcccgcgtta caagatcatg cgcggatatc
     2281 acgtctcgcg ccgcggcggc tgggacacgc atggcttacc ggttgagatc gaagtagaga
     2341 agcagcttgg cttcaccaac aaacagcaga tcgaagaata cggcgttgat aaattcaacg
     2401 agctgtgcaa aaaatcggtt ttcaactaca tccaggaatg ggagaagctc accggtcgca
     2461 tcggcttctg ggtggattta gacactgcct acgtgactta caccaacgac tacatcgagt
     2521 cggtgtggtg gatcttaaaa aacttctggg agaaggactt gcttttcaag ggctacaaga
     2581 tcgtgccgta ttgcccgcgt tgtggcacgc ctctctctga ccatgaagtt gctcttggct
     2641 acgaagatac ggctgacccc tcggtctttg tgcgcttccc attagtggac aagcccgata
     2701 cttcattctt agtttggacg actactccgt ggaccttgcc tgccaatgtt gccatcgccg
     2761 ctcaccccga tgtggattat gtgactgtcg aacgtgataa caacggcacg acggaaaaac
     2821 tcatcctcgc caaatcactg ttggaaaaag tcttccgcgg tgaagaagta aaagttgtgg
     2881 atacattcaa aggcaagaag ttgaagggca tgaaatatca accgctcttt acctttgtgc
     2941 cgctcgataa acccgcacat tatgtcgtgc tcggtgattt cgtcactact gaagatggta
     3001 ccggtcttgt gcaccaggct cctgctttcg gtgcagaaga tatggaaatg tcgaagcaat
     3061 acgatttgcc tgtcttgctt acgatccaac cggatggtgg gtttatccct gaagtcacgc
     3121 cgtggcgcgg agttttcttc aaagatgccg acccgcagat catacaaaac ctcgaagcgc
     3181 gcggactgat gttccgctcg gagaagtacg tccacacgta tccattctgc tggcgttgcc
     3241 atactccttt gctgtattac gcccgcgact cgtggtacat ccgcaccagc gcgaagaagg
     3301 aaaatctcgt caacctaaac aagaccatca actgggtgcc cgatcacatc aagaacggac
     3361 gcttcggcaa ctggcttgag aacaacatcg attggtcgct ctcgcgtgaa cgctattggg
     3421 gcacgccact tcctgtttgg gaatgtgaga acggcgaatg taaacatcgc gagtgtgttg
     3481 gctctgttgc tgagctttct gaaaaagccg gtcgcgacct gactgagctt gatctgcacc
     3541 gcccgcacgt agataacgtt cactggaagt gtgtcgattg cggtggacaa atgacgcgtg
     3601 tcaaagacct gattgacgtg tggtttgact ctggttccat
//
LOCUS       DAOREF010000205         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15941_length_3628_cov_4.265043, whole genome shotgun sequence.
ACCESSION   DAOREF010000205 DAOREF010000000
VERSION     DAOREF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15941_length_3628_cov_4.265043"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKI33_05550"
     CDS             <1..638
                     /locus_tag="PKI33_05550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNM36503.1"
                     /translation="ESEADAENMVEKLRKQVKLFQVESEVSKKLARNKQIEMEAVLAQ
                     AQEVAHVDPLTFLPNRRTIIKDLQSEVMRAERYHSKFCISVVDIDKFKSINDTYGHPV
                     GDEVLRHVALQLRDGIRHPDVVGRYGGEEFIILLPNSDNNAAAEQASRLCRQIRSSVV
                     RSKDHDIQVTISVGIAQFHIGEDSWHSLLKRADNAMFEAKSRGRDRWVVGD"
     gene            complement(683..1906)
                     /locus_tag="PKI33_05555"
     CDS             complement(683..1906)
                     /locus_tag="PKI33_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNM36504.1"
                     /translation="MTTLLPRRQFLQGCSAAIAAMAGAKLTNLAFAKQDNPSDILVVI
                     FLRGGWDALNVVPPIDGDDRGFYEKARPDLKISSLLRLNDQFGLHPAMSPLYDLYQAG
                     KAGIIHSVGLNYDTRSHFDAQDYIELGTPGSKSTTSGWITRHLQTTGISSILPVVSTT
                     GQPMSLLNFVPTVSVSTPDEFSLWDNGLRDSHMNALRQLYAGESLIHRAGSRTLDALD
                     IVRPLNGNTYQPANGAYYNDDELGNQLKTIAQMIKLDAGLRVAAVDFGGWDTHEYQNS
                     GNEGYMADLLNSLASGLANFYLDLDSGYTEKLSVVVISEFGRRLVQNDSYGTDHGHGS
                     VMLTLGGNVNGGQVFGSFPGLHNDQLYDHADLAITTDYRSVLGELLSKRMGNADLESV
                     FPGFSGNYNPLGIYR"
     gene            complement(1911..>3628)
                     /locus_tag="PKI33_05560"
     CDS             complement(1911..>3628)
                     /locus_tag="PKI33_05560"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNM36505.1"
                     /translation="TRRDFLKFGGALAATSALAACAPGEENAITQPFEGTTTLQNPVI
                     APEAQTSLPPVSMAILALSRLTFGIRPGDVDAFNALGATDDERLSAFVTQQLNPDSID
                     DSEFEARYANAGFETLHKSHEQLYADHIASNPYDSNDDAYWQWYSKPTYELVDATILR
                     AVYSKKQVVELLADFWHNHFNVYFWQDDGVPLLVSYNRDVLRAHMLGNFRQMLEAVAR
                     HPSMLYYLNQNNSSDAGPNENFARELFELHTLGAENYLGVRDPNTVEKDANGIAVGYV
                     DNDVYESARCLTGWRVDDDLGDWEDGVEKTGNFLYYKPWHDRFNKLVLGKYIPADQED
                     MKDAHDVLDLLAYHPGTARHIARKLCTRFISDVPSESIVQAAANTFIQFKDAPDQLKR
                     VMETILLSAEFKASWGGKVKRPFEAAISMIRALNSETVRISGKLNWMLSLMGQAIFER
                     RTPDGYPDTRDAWANSMSLLYRWNLAVSLAENWMDDDDTGFKIASDVYGQTPAEIRSA
                     SSLADYWIQRILNRTLPDESRNAIIAVMAQEYGWDETLPEDHVHYVLPAMVEVILMSP
                     DFQWR"
BASE COUNT          934 a          852 c          940 g          902 t
ORIGIN      
        1 tcgaatcgga agccgatgca gagaacatgg tggagaagct ccgtaaacaa gtgaaattgt
       61 ttcaggtgga aagcgaagtt tcaaaaaaac tagcacgcaa taaacagatt gaaatggaag
      121 cggtcttggc gcaggcgcaa gaagtagcgc atgtcgaccc actgaccttc ctgccgaatc
      181 gccgtaccat catcaaagac ttgcaaagtg aagtgatgcg tgctgaacga tatcattcta
      241 agttttgtat ttcagttgtg gatattgata aatttaagtc cattaatgat acatatgggc
      301 acccagtagg ggatgaagtg ttacgccatg tggcacttca attgcgagat ggcatccgtc
      361 accccgatgt ggtgggacgt tatggcggcg aagagttcat cattctgctc ccaaactcag
      421 ataacaatgc tgcggctgaa caagcctcac gtttgtgcag acagatacgc agttctgtgg
      481 tccgatcgaa agaccatgat attcaggtga ccatcagtgt ggggattgct caatttcaca
      541 tcggcgagga ttcatggcat agcttgctca aacgggcaga caatgccatg ttcgaagcca
      601 aaagccgtgg acgtgaccgt tgggtcgttg gagattaaat aaaaagagcc tgtctcatgg
      661 agataggctc tttttcaaat agttatctgt aaatcccgag cgggttgtaa ttgccagaaa
      721 agccggggaa gacagactca aggtcggcat tgcccatgcg tttggaaagt aactctccca
      781 atacggagcg ataatctgta gtgatggcaa ggtcagcatg gtcgtatagc tggtcattat
      841 gcaaaccggg gaaggaaccg aagacttgcc cgccattgac gttaccgcca agcgtaagca
      901 tcacactgcc atgtccatga tcggtgccgt aggagtcgtt ttgaacaagc cgacgcccaa
      961 attcactaat gacaacaacc gataatttct cggtgtatcc agagtctaag tctaggtaaa
     1021 aatttgccaa cccagacgcc agactgttga gcaaatctgc catgtagcct tcattgccgc
     1081 tgttctgata ttcgtgcgta tcccaaccgc caaagtccac ggcagcaacg cgtaagccgg
     1141 catcgagttt gatcatttga gcaatggtct ttagttgatt gccaagttca tcatcattgt
     1201 agtaagcgcc attggcgggt tgatatgtat ttccattcaa cgggcggacg atatccaagg
     1261 catccaatgt ccgcgagccg gcacggtgaa tgaggctttc cccggcgtag agttggcgca
     1321 acgcattcat gtgcgagtcg cgcaagccgt tatcccataa tgagaattca tcaggcgtgc
     1381 tcacactgac tgtgggaaca aaatttaaca acgacatagg ctgccctgtg gtggaaacca
     1441 ctggcaagat cgaagaaatg ccggttgtct gtaagtgacg ggtgatccac cctgaggtag
     1501 tgctcttgga accgggcgtt cccaattcaa tatagtcttg agcgtcgaag tgactgcgcg
     1561 tatcgtaatt caaccccaca gagtgaatga tgcctgcttt accggcttga tacagatcgt
     1621 ataacggact catggcaggg tgcagaccaa attggtcatt caagcgcaat agactggata
     1681 tcttcagatc ggggcgggct ttttcgtaaa agccacggtc atctccatca ataggcggaa
     1741 caacattgag cgcatcccaa ccgccgcgta gaaatatgac gacgaggata tcgcttgggt
     1801 tatcttgttt agcaaaagcg aggttggtca actttgcgcc cgccatggcg gcaatggcgg
     1861 ctgaacatcc ttgcaggaac tgacggcgtg gaagtaaagt ggtcattggg ttatctccat
     1921 tgaaaatcgg ggctcattaa aatcacttcc accatggcag gcagaacata gtgcacgtga
     1981 tcttcaggca gggtttcgtc ccaaccgtat tcttgtgcca tcactgcgat gatggcattg
     2041 cggctttcat cgggtagcgt acggttcagg attctttgaa tccaataatc ggcgagagaa
     2101 gaggcgctgc gaatctccgc cggggtttgc ccataaacat ccgaagcgat tttgaagccg
     2161 gtatcgtcat catccatcca gttttctgcc aaacttaccg ccagattcca gcgatacaag
     2221 agcgacatgg agtttgccca tgcatcgcgg gtgtcgggat agccatcagg tgtgcggcgt
     2281 tcaaagatgg cttgacccat cagactcaac atccagttga gtttgcctga aatgcgcacc
     2341 gtttctgagt taagcgcacg tatcatggat attgccgcct caaacggacg tttgaccttt
     2401 ccgccccagc tggctttaaa ctccgctgaa agcaggatgg tctccattac acgcttgagt
     2461 tggtctggtg catctttgaa ttgaataaaa gtattggctg cagcctgaac aatcgactcg
     2521 gatggcacat cggaaatgaa gcgtgtgcaa agttttcttg caatgtggcg agccgtgccc
     2581 gggtgatagg ctaaaaggtc gagtacatca tgagcgtctt tcatatcttc ctgatcggca
     2641 ggaatgtatt tgccgagcac gagtttgttg aaacggtcat gccagggttt gtaatacagg
     2701 aagttgcctg tcttttcaac accatcttcc caatcaccga gatcatcatc cacccgccag
     2761 ccggtgagac agcgcgcaga ctcgtagacg tcgttatcaa catagccgac ggcaatgcca
     2821 ttcgcatctt tctcaaccgt gtttgggtcg cgcacgccca gataattctc tgcgccgagt
     2881 gtgtgaagct caaataattc gcgtgcgaaa ttctcgttgg gacctgcgtc gctgctgttg
     2941 ttttgattca agtagtacaa catagaaggg tgccttgcga cggcttccaa catttggcgg
     3001 aagttgccaa gcatatgggc acgcaacaca tcgcggttgt atgaaaccag cagtggcacg
     3061 ccatcatcct gccaaaaata gacattgaaa tggttatgcc agaaatctgc gagaagttcc
     3121 acaacttgtt ttttgctgta tacggctcgt aagatggtgg catctactaa ttcataagtg
     3181 ggtttgctat accattgcca gtatgcatcg tcattagagt cgtatggatt gctggcaatg
     3241 tggtctgcgt ataactgttc atgcgattta tgcaaggtct caaacccggc attggcataa
     3301 cgtgcttcaa actcactgtc atcgatgcta tctgggttga gttgttgagt tacaaaagct
     3361 gaaagacgtt cgtcatctgt tgcgccaagg gcattgaaag catccacgtc accggggcga
     3421 atgccaaatg taaggcgact caaagctagg atcgccatcg aaacaggggg aagagacgtt
     3481 tgtgcttcgg gtgcaataac cgggttttgt agagttgttg tcccttcaaa aggctgcgtg
     3541 atcgcgtttt cttctcccgg cgcacaagct gctaaagcag atgttgctgc caaggctccg
     3601 ccaaacttta gaaaatcacg tcgcgtta
//
LOCUS       DAOREF010000206         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_15953_length_3626_cov_4.378325, whole genome shotgun sequence.
ACCESSION   DAOREF010000206 DAOREF010000000
VERSION     DAOREF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15953_length_3626_cov_4.378325"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKI33_05565"
     CDS             <1..817
                     /locus_tag="PKI33_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36506.1"
                     /translation="QTGIVGYVTATGQPRIALDVGADSAYFDNPDLPNTRSEIALPLR
                     YAKQIIGALDVQSLEANAFQADDIAILTTLADQVTVAINNALTIAEAKKTLAEAQAAI
                     GQVAEEAWQVLRPTRLGLGFAYSESGVKPLQNPFDNVQAHEAFQKGQTMFTTSPDHKT
                     VLAVPIRLRGSVIGVMQLSNRSNYEKLTQDDAEIAEAVAERLSLAIETATLLQSTQHR
                     ADIERITANITSKVSSSSRFETILQTAAQELSRALGGSDVLVQIEPAALKMYT"
     gene            842..3157
                     /locus_tag="PKI33_05570"
     CDS             842..3157
                     /locus_tag="PKI33_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36507.1"
                     /translation="MDQTAQTPPEETNFPEHLQQTSLGLKLTLIAVGLLLVAFIVYTF
                     ISIRISQGDLLASVEENLKIKNNETISLIQSRLTEAKGIATNLSVAVESGAFTEGDLR
                     VIIQNTVDRNASIHGATVGYEPNQFKSNIYYWSPYYYRMPDNTIQYLQLGDYDYFNQE
                     WYSVTKKNSAPTLLPPYRNKSHGETWISTWSIPIYNNQGDFKGVAAVEIDLSEIQQTF
                     TSLKFGRTGYAFMIDSSGTILGIGDESGNFEPMIDSMYVIANTNPASNWITLIDKMTN
                     GEAGYVEVNDRNGDPMFVSYAPVGMDTDWSLALVYPREEVIQQTRRLQASLTAYSFGL
                     ALIFGLVIFFFTRTITNPLEQLTKVAEEISNGNLNISAPVESDDEVGTLAETINRMTA
                     QLQDTYNNLERRITERTVDLEIARRQSEIRTTQLLSIGEISKIINSEQELDILLPLIA
                     RLVSERFNFYHVGIFLIDDARQYAVLQAANSSGGQNMLKRGHKLKVGESGIVGYVAKT
                     GVPRIALDVGQDAVFFNNPDLPNTRSEVALPLKVRENIIGVLDVQSERPGAFSEDDTN
                     IFNILADQVAITLENTRLFEQTQQALDEVRSAYQRNLQEGWKVFGQEEGIIGYQQSIT
                     GGKKLAKPVHLDEIQQAMHRGQVQEFHPDGKTGEPTLVVPIKLRDQIIGVMHIKAPSK
                     ERLWTQDETNLVEAVSERLSLALENARLIQESQRQVIKEQTITEITSRIGSSINLDNV
                     LLTAVEELGRNIPGSEVTIKLKNENKNDGSE"
     gene            3141..>3626
                     /locus_tag="PKI33_05575"
     CDS             3141..>3626
                     /locus_tag="PKI33_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36508.1"
                     /translation="MMAVNKEKKQAMKQRFSIQDLLAKTPLGNLNLRGKLTAGNMIIT
                     LLVIAVMGIYVYFRIQTASQQLITSVDENARNRAEEDLVNTNREQATFLDSFFVNMTN
                     NTSIATAAIRNILDDSQLANSTYWDANTQLIRLENGSFDNQNAEITSIFIPVNVNLNN
                     AL"
BASE COUNT         1121 a          822 c          753 g          930 t
ORIGIN      
        1 acaaacaggt attgtggggt atgtaactgc aacagggcaa ccccgcatcg ctctggacgt
       61 gggagcggat tctgcttact ttgacaatcc tgacctccca aacacccgct ctgagatcgc
      121 tttgcctctg cgctacgcca agcaaattat tggcgcatta gatgtacaaa gtttggaagc
      181 caatgcattt caagcagatg acattgccat attaacaaca cttgcagatc aagtaacagt
      241 tgctattaat aatgctttga ccattgcaga agccaaaaag acattggcag aagctcaagc
      301 ggcaattggt caagttgcag aagaagcctg gcaggtttta cgccccactc gccttgggct
      361 aggctttgca tattctgaat ctggtgtaaa gccacttcaa aacccgtttg ataatgttca
      421 ggcacacgaa gcctttcaaa agggacaaac catgttcacc accagcccag atcacaaaac
      481 ggttctggct gttcctatcc gcttgcgtgg aagcgtaatt ggcgtaatgc aattgagcaa
      541 ccgcagtaac tatgaaaaat tgacccagga tgatgcagaa atcgcagaag cggtagcaga
      601 acgcctctct cttgcgattg aaacagcaac cttgcttcaa tctacacaac atcgtgccga
      661 tattgagcga atcacagcca acatcactag taaagtcagt tcttcatcta gatttgaaac
      721 catccttcaa acagcagctc aagaattaag ccgtgccttg ggcggctcag atgtgctcgt
      781 tcagatcgaa ccagccgcgc tgaagatgta tacctaatcg tcttacacaa agagaatttt
      841 tatggatcaa accgctcaaa cacctccaga agaaacaaat tttcccgagc atctacaaca
      901 gacaagcctc gggttaaagc taaccttaat tgcagttggc ttacttctgg ttgcatttat
      961 tgtgtacacc tttattagta ttcgcattag tcaaggcgat cttttagcaa gcgtagaaga
     1021 aaatttaaaa atcaagaaca atgagaccat ctctcttatt caaagccggt taacagaagc
     1081 aaaaggaatt gcaacaaact tatccgttgc agtagagtct ggcgctttta cagaaggtga
     1141 tctacgggta attattcaaa acacagttga ccgcaatgct tctattcatg gtgcaaccgt
     1201 tgggtatgaa ccgaatcaat ttaagtccaa tatctattat tggtcgcctt attattacag
     1261 aatgccagat aacaccattc aatatcttca attaggcgac tatgattatt tcaaccaaga
     1321 gtggtactcg gtcactaaga agaattcggc acctacatta cttcccccct acagaaacaa
     1381 aagccatgga gaaacttgga tttctacatg gagcatccct atttacaaca atcaaggtga
     1441 tttcaaaggt gttgctgccg ttgaaattga cttgagcgag attcaacaaa cctttactag
     1501 tctaaagttt ggtcgaacgg gttatgcctt catgatcgac tcaagcggaa ccatactcgg
     1561 cattggcgat gaaagcggta attttgagcc gatgatcgat tccatgtatg tgatcgcaaa
     1621 tacaaacccc gcctcaaact ggatcacact catcgacaaa atgacaaatg gcgaagcagg
     1681 gtatgtagaa gttaatgatc gcaacggcga ccccatgttt gtctcgtatg ctccagttgg
     1741 tatggataca gattggtctt tggcattggt gtatccacgt gaagaggtaa ttcaacaaac
     1801 tcgccgatta caagcctctc tcactgccta cagttttggg ttggctctta tctttgggtt
     1861 agttatcttc ttctttaccc gaacgatcac aaacccgctt gaacaactca caaaggtcgc
     1921 tgaagaaata tcaaatggaa atttaaatat ttccgcccct gttgaaagtg atgatgaagt
     1981 aggcacatta gctgagacca tcaaccgtat gacggctcag ttgcaggaca cctacaataa
     2041 tcttgaaagg cgtatcacag aacgtacagt agacttagag attgcacgcc gccaaagtga
     2101 aattcgcacg acacaacttc tttccattgg cgaaatctca aaaatcatta actctgagca
     2161 agaattagat attctgttac cgcttattgc tcgtttggtt agtgaacgtt ttaatttcta
     2221 tcatgttggc atcttcttaa tcgatgatgc tcgtcaatat gccgtccttc aggcagccaa
     2281 cagctccggc gggcaaaaca tgttgaagcg tggacacaag ctcaaagtcg gtgagagcgg
     2341 tatcgtgggg tatgtcgcta aaactggcgt tccacgcatc gccctagatg taggtcaaga
     2401 tgctgtcttc ttcaacaatc cagatcttcc taatacacgt tctgaagtgg cgcttcctct
     2461 caaggtgcgt gaaaatatta ttggtgttct agatgtgcaa agcgaaagac ctggcgcatt
     2521 cagcgaagat gacacgaata ttttcaacat tctggcagac caagtggcaa ttacacttga
     2581 aaacacacgc ctctttgaac aaactcagca agcacttgat gaagtgcgat ccgcgtatca
     2641 acgcaacctt caagaaggtt ggaaagtttt tggtcaagag gaaggcatta ttggatatca
     2701 acaatccata accggaggca aaaaattagc caagccggtt cacttggatg aaatccaaca
     2761 agccatgcat cgtggacagg ttcaagagtt tcatccagat ggaaagaccg gcgaacctac
     2821 cttggttgtt ccgatcaagc ttcgtgacca aatcattggt gtaatgcaca tcaaagcgcc
     2881 gtcgaaagaa cgtttatgga cacaagacga aaccaatctt gtcgaagctg tttccgaacg
     2941 cctctcgctt gcgcttgaaa atgcacgcct gattcaagaa tcacaacgtc aggtcattaa
     3001 agagcaaaca atcactgaaa ttacgagccg tattggttca tccatcaatt tagataatgt
     3061 acttctcact gcggtagaag aattaggacg taacattccc ggctcagagg taaccatcaa
     3121 attgaaaaac gaaaacaaga atgatggcag tgaataagga aaaaaaacaa gccatgaagc
     3181 aaagattttc aattcaggac ttgcttgcta aaactccgct tggcaacctc aatttacgag
     3241 gaaagctaac tgcgggaaat atgattatta ccctccttgt cattgcggtt atgggtatat
     3301 atgtatattt tcgtatccag acagccagtc agcaattgat caccagtgtg gatgaaaatg
     3361 cgcgcaaccg tgcagaggaa gatttagtca ataccaaccg cgaacaggca accttccttg
     3421 atagcttctt cgtcaacatg acaaacaata catccattgc tacggctgca attagaaata
     3481 tattggacga ttctcaatta gcaaatagca cctactggga tgcaaacact cagttgatac
     3541 ggttggaaaa cggcagtttt gacaatcaaa acgcagaaat aacctctata tttattcctg
     3601 tcaacgttaa cttaaacaat gcactg
//
LOCUS       DAOREF010000207         3620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16004_length_3620_cov_4.231978, whole genome shotgun sequence.
ACCESSION   DAOREF010000207 DAOREF010000000
VERSION     DAOREF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16004_length_3620_cov_4.231978"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2743)
                     /locus_tag="PKI33_05580"
     CDS             complement(<1..2743)
                     /locus_tag="PKI33_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36509.1"
                     /translation="MSAATRSSKDWLQLASLGEQLRSEDSLSAQRDRIAAMANRLIEG
                     KVDVWLHENIFRLPDWDDGYIFPTQPALDGMKRAIKTGKLARKKKSSGKKPPASRSTF
                     AAIPLEDQGITLGALQVSRSKGPEFTDDELNLLQGLGHIISVNLFASHRAEVEQFRLR
                     QLNLVREVSAQIANVLDVDDLASQVTRLIQKTFNFYYVAFFTLDPQDNALHFRASAVA
                     PRKGRRKASIALEVEIGQGLIGEAAKTGMEIVCDDVQSDSRYRFIDSLPETRSEVVIP
                     LKIEDHILGALDVQSNQPSAFHPIDLLILRALADNVARAVDGAKLYSALSRRANQLTL
                     VSEVSKSVTSTLDLSKLMRDAASLIHEKFGYPHVSLFTVHPNRRMIMYEAGSGKRSKK
                     LEGFTIPLDDPKGIMPWVARHGKTALANDVSKDSRYIQSPLPPKNTKSELCVPLIFNE
                     RVVGLLDIQSDQLNAFTEDDRVMFEAVADTMAAAIRNADLYRSEQWRRQVADSLRDVA
                     GLVSSNAGLDEVLEMILAELDRNLPIDVSAIWLLQEGELCLSAVHGIDGAQLENTCLA
                     FPESTYVMAEALESDVPIIRRPEDPMGPAGLTMNFDPTYSSIVAPLRVGDRPLGVLAL
                     AHHTSGRYGHEARAIAGTFANYAAVAMENTRLFDAAQEQAYASAALLQVAQAVVSLSD
                     LDEILGSIIRIMPILVGVKRVVLYRLDMEYQTLQASHEYGFSEEEEAVITEKILPVAD
                     FPLIVWALEQNTLVTYPLKAEATPLDWFAIQPGSEEEAHSIDADRLLIAVPLTIKHDI
                     FGVLLIEEDENARRFRSRRLEIINGIAQQAALAIQNDLLQQEMVVRERLETEVQLARQ
                     IQQTFIPQSLPDHPAWQLAACLRTARQVGGDFYDVIELPNNKLGIFIADVADKGMPAA
                     "
     gene            complement(2748..3155)
                     /locus_tag="PKI33_05585"
     CDS             complement(2748..3155)
                     /locus_tag="PKI33_05585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM36510.1"
                     /translation="MPAYTFPAKFEFLDEIRDTVAEIARSGGFSEKTIYSLQLAADEA
                     ASNIIEHAYEGVSNATLYMTCDMKGDEIVITMRDTGKSFNPTTVKEPNLKADLSERQI
                     GGLGVYLMRKLMNTVHYESSKSGNLLTMTKRRE"
     gene            complement(3225..3560)
                     /locus_tag="PKI33_05590"
     CDS             complement(3225..3560)
                     /locus_tag="PKI33_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM36511.1"
                     /translation="MEITTQEFKHCDLIAVKGRVDSATAPQFAKALETANDNGRFKLA
                     LDMSELEYMSSAGFRALLAAQRNCKRYNRGEVVLVRVPDRIREALELAGFTELFKTFD
                     DTVTAVGSF"
BASE COUNT          917 a          903 c          892 g          908 t
ORIGIN      
        1 aagcggcagg cattcctttg tccgcaacat ctgcaatgaa gatgcccaac ttattattag
       61 gcaattcgat tacatcataa aaatcgccgc ccacctgacg ggctgtgcgc aagcatgctg
      121 ccagttgcca tgcagggtgg tcaggtaaag attgcgggat gaaggtctgc tgaatttggc
      181 gagccagttg aacttccgtt tcgaggcgtt cgcgcaccac catctcttgt tgaagcaaat
      241 cattctgaat cgccagcgct gcttgttgag caatgccatt gataatttcc aaacggcgtg
      301 agcggaaccg gcgcgcattc tcatcctctt cgatcaagag gacgccaaaa atatcatgct
      361 tgatggttaa cggcaccgca atcaacaaac gatccgcatc gatgctatgc gcttcttcct
      421 ccgacccagg ctgaatcgca aaccaatcca acggcgtcgc ctcagccttg agcggatagg
      481 tcactaatgt attttgctcc agcgcccaca caattaatgg aaaatcagca acgggcaaaa
      541 ttttctcggt gataacggct tcctcttctt cagagaaacc atactcgtgc gaagcttgta
      601 aagtttgata ttccatatca aggcggtaca acacgactcg cttgacaccc accaagatcg
      661 gcataatacg aataatggag ccgagaattt catccaaatc gctcaaactg acaacagctt
      721 gtgccacttg caacaaagca gcagaggcat aagcctgctc ttgagcagca tcgaagaggc
      781 gggtattttc catagcaact gccgcataat ttgcgaaagt acccgcaatc gcacgcgctt
      841 catgtccata acgcccagat gtgtggtgcg ccaatgccaa gacgcccaat ggacggtcac
      901 ccacccgcaa tggcgccaca atggatgaat acgttgggtc aaagttcatt gtcagcccag
      961 cgggacccat tgggtcttca gggcggcgga tgataggaac gtccgactca agcgcctcag
     1021 ccatcacata cgtagattca ggaaatgcca gacaggtatt ttcaagttgt gcaccatcaa
     1081 tgccatgcac agccgagaga cacaactcac cctcttgaag caaccaaatt gcagaaacat
     1141 caattggaag attgcggtct agctcagcaa ggatcatttc aagcacttca tccaacccag
     1201 cattgctgga aaccaagcca gcgacatctc gcaaactatc ggcgacttgg cgacgccatt
     1261 gctcagaacg atataagtct gcattacgga tggcggcagc catggtatct gctaccgctt
     1321 cgaacatgac ccggtcatct tctgtgaatg cattgagttg gtctgactga atgtccaaca
     1381 aaccaacaac gcgttcgtta aaaatcaacg gaacacataa ctctgacttc gtattttttg
     1441 gcggcaaagg cgattggatg taacgcgagt cttttgaaac gtcattcgca agcgcggttt
     1501 ttccatggcg tgccacccaa ggcataatgc ctttcgggtc atctaatggg atggtgaagc
     1561 cttcaagttt tttgctacgt tttccacttc ccgcttcgta cataatcatg cgtcgattcg
     1621 ggtggacggt gaaaagcgaa acgtgaggat aaccaaattt ttcgtgaatc aagctagcag
     1681 catcgcgcat tagctttgaa agatcgagcg tagatgtaac acttttactt acttcagaaa
     1741 ccaaggtcag ttgattggcg cgtcggctca gggcgctata gagtttggct ccatctacag
     1801 cgcgggcaac attatcggca agggctcgta gaatgagcaa gtcaatagga tgaaaagcgc
     1861 ttggctgatt gctctgaaca tccaatgcgc ccagaatatg gtcttctatc ttgagcggaa
     1921 tgacaacttc agagcgagtt tctgggaggc tatcaataaa gcgataacga gaatccgact
     1981 gaacatcgtc acagacgatc tccatgccgg tctttgctgc ttcaccgatc aacccctgac
     2041 cgatctccac ttccaatgca atggatgctt tacggcgacc tttacgcggt gcaacagcgc
     2101 tagcacgaaa gtgaagcgca ttatcttgag ggtcgagagt gaaaaaggca acgtaataaa
     2161 agttaaaggt cttttggata agtcttgtta cttgagatgc tagatcgtcc acatcgagca
     2221 cgttggcgat ctgagcgctg acctcgcgta ccaagttcaa ttgtcgcaag cggaattgtt
     2281 ccacttcggc gcggtgcgag gcaaataaat ttacagagat gatatgcccc aagccttgaa
     2341 gcaggtttaa ttcatcatca gtaaactctg gtccttttga gcgggatact tgaagcgctc
     2401 ccagcgtaat cccctgatct tcgaggggga ttgcagcaaa ggtcgagcga gaggcggggg
     2461 gtttcttccc gctgcttttt ttcttacgag ccagtttccc cgtttttatg gcacgtttca
     2521 ttccgtctaa tgcaggctgg gttgggaaga tatatccatc gtcccaatct ggcaggcgga
     2581 agatgttttc gtgcagccag acatccacct tgccttcgat gaggcggttt gccatagcag
     2641 cgatgcgatc tcgttgcgcg ctaagagagt cttcgctccg cagttgctca cccaagctgg
     2701 cgagttgaag ccaatcctta gaggatcggg ttgcggcgct catgaaacta ttctctccgt
     2761 ttggtcattg tcagtagatt gccagatttg gaagattcgt aatggacggt gttcatcaac
     2821 ttccgcatga gatatacgcc cagcccgccg atctgccgtt ctgataggtc tgctttgagg
     2881 ttcggctctt tgaccgtggt agggttgaat gactttccag tatctcgcat ggtgatgacg
     2941 atctcgtccc ctttcatatc acacgtcata tacaaagtag cgttcgacac accttcatag
     3001 gcatgttcaa tgatgttaga agcagcttcg tccgcggcaa gttggaggga gtaaatggtc
     3061 ttttcagaga accctccgct gcgcgcaatc tctgcaactg tatcgcggat ctcgtccaga
     3121 aattcaaact tggctgggaa tgtgtatgct ggcataaaga aaatgagatg ccgtcccttg
     3181 tctttaaaga gacggcatgt cggttctttt taggaaccct tctattagaa acttccgacg
     3241 gcggttacgg tgtcgtcgaa ggttttgaag agttcggtga agcctgccaa ctcgagggct
     3301 tcgcgaattc ggtcgggaac acgaacaagc accacctcgc cgcgattgta tcgtttgcag
     3361 ttgcgttgtg ctgcgagcaa ggcacggaaa cctgcgctgg acatgtattc caattcgctc
     3421 atatcaaggg caagtttgaa acgtccgtta tcgttggcag tttcaagtgc cttggcaaat
     3481 tggggagctg tcgcgctgtc aacccgtcct tttactgcaa tcaggtcaca gtgcttgaat
     3541 tcttgggtcg tgatttccat aatttctcct ccgaacaaga tatcgagtga tgaattagcg
     3601 ggattatatc acgtagataa
//
LOCUS       DAOREF010000208         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16036_length_3616_cov_3.657119, whole genome shotgun sequence.
ACCESSION   DAOREF010000208 DAOREF010000000
VERSION     DAOREF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16036_length_3616_cov_3.657119"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..358
                     /locus_tag="PKI33_05595"
                     /pseudo
     CDS             38..358
                     /locus_tag="PKI33_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
     gene            355..1224
                     /locus_tag="PKI33_05600"
     CDS             355..1224
                     /locus_tag="PKI33_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNM36512.1"
                     /translation="MSITFASILCGISGMFGWGIYDFLGGVFAKKIGSFKSLFWSQLA
                     GLVSLIVLAIFLKPNFHLPLLALALFPIASIVYSAGYLFFFKGFEVGNVSIVAATMNL
                     WAVFTMIFAFTFMGQRLSSTQTIGVLLILAGVLLASVNWSEIREQKFQLSAGVREAVA
                     GAFFFGIFWNISEIVSEEIGWLYTTLLVKLGIIVFLLLFSAIAKRELGLGKIASKTKS
                     AILLMGIIEAGAVAIVNYGLTIGDAILITPIASALSIVTILLAIIFLKDKITKIQGLG
                     IATAITGIVVTGF"
     gene            complement(1221..1715)
                     /locus_tag="PKI33_05605"
     CDS             complement(1221..1715)
                     /locus_tag="PKI33_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM36513.1"
                     /translation="MNAEAFRHFFNYHFAENRKILEHVAALTFEQFTQKVDYSRGSIR
                     EQLIHLIDAEDVWISELRGVQPSEPLPETTEVDDRDAIRSLWDVVEQNTRTYLANLQD
                     NQLVSKPITDPEEDKELIVWQVLLHIVNHATDHRAQLLRALHDLGVDTKSQDYIFYVY
                     ENPL"
     gene            1776..2198
                     /locus_tag="PKI33_05610"
     CDS             1776..2198
                     /locus_tag="PKI33_05610"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNM36514.1"
                     /translation="MTKISTSLTINASQEAIWKVLSDVAHWQEWTPTVTKVEMLDTPE
                     IKLGNHYKVIQPKLQPVVWTVTELNSANFTWESKSPGMHMEAEHVVKSINANQSEVTL
                     TFAFNGWLGNLIGRMYGKMTEEYIRTEAQSLKKKAESA"
     gene            2212..2838
                     /locus_tag="PKI33_05615"
     CDS             2212..2838
                     /locus_tag="PKI33_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNM36515.1"
                     /translation="MGVTTFKSIGEYATLRFVESKVFGAFPTIVVQDSPEFIVLYMPT
                     GVLGKNVDHKPATNELFSADKIDIIDFQWKRTDVLMLIVPGEAFSTYVMWDAGTKNLD
                     CWYVNLQEPIRRTSIGFDTMDNMLDVVISPDMNTWQWKDDDEFEAAQSIGFYSAEKAR
                     AIRAEGEKAVQLITKERRTLYEKWKTWEANPEWELPILSPDWQRMDLG"
     gene            2840..3529
                     /locus_tag="PKI33_05620"
     CDS             2840..3529
                     /locus_tag="PKI33_05620"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNM36516.1"
                     /translation="MATNRVLNWQGCNNVRDLGGLTASNGSKTRWGAVVRGDQPAKLT
                     PEGWGELYEHGIRTIVSLRTYGFNEKDHPVVTPPYPDIHVMSVEIEDVTNKKFVEKWA
                     TSDLWGTPLYWTDALNLWANRHADALKAIAQAQPGGVFFHCIRGHDRTGIVSFLLLSL
                     LGVSLKDITADYELSADPVRDKLLAERNTTAHEVLQKTHAGINIENYLLNGGMTQDDL
                     AALRARLLTNK"
BASE COUNT          951 a          952 c          834 g          879 t
ORIGIN      
        1 cagccggata ctatgacgcg tattacttga aggcacaaaa gtgcgcacgc ttcttcgtcg
       61 ggactttgaa actgcctttt ccaacgtgga cattctcctc gccccgacct gtcctaccac
      121 cgccttcaaa ctcggcgaga aaaccagcga cccgcttgaa atgtacctct cagatattta
      181 cgtcgtttca acgaatcccg ctggcgtccc tgccattgca ctccccgctg gcttctccaa
      241 caacatgccc gtcggtatgc aactcatcgg caaacacttg gacgaagcca ccctcttcca
      301 agtcgcccac gcctaccagc aagtgactga ttggcacaag caacaaccaa gtttatgagc
      361 attaccttcg caagcatcct gtgtggaata tcaggcatgt tcggctgggg aatctacgat
      421 ttcctcggcg gcgtcttcgc taaaaaaata ggctccttta agtctctgtt ttggtcgcag
      481 ttggctggct tggtttccct aattgtgctt gcgatattcc tcaaacccaa ctttcattta
      541 cccttgcttg cgctggcttt atttccgatc gcttccatag tttattcggc agggtatttg
      601 ttctttttca aaggctttga agttgggaat gtttccatcg tcgcagcgac catgaatctg
      661 tgggcagtgt tcacgatgat cttcgccttc acattcatgg gacagcgtct ttcctccact
      721 caaaccatcg gcgttctgct aatcctcgct ggcgtcctgc tggcatccgt caattggagc
      781 gaaattcggg agcagaaatt tcaactctcg gcaggcgtca gggaagcggt ggcaggagcg
      841 ttcttcttcg gcattttttg gaacatcagt gaaattgttt ccgaagagat cggatggctg
      901 tacacgaccc tgctcgtaaa attgggcatt atcgtttttc tcctgctttt ctcagctatt
      961 gccaaacgag agttggggct gggaaagatt gcatcgaaaa caaaatccgc catcctgctg
     1021 atgggcatca tcgaagcggg cgcagtggca atcgtcaact acgggctgac catcggcgat
     1081 gcgatcctga tcacgcccat tgcatctgcc ctttccatcg tgaccatttt attggcgatc
     1141 attttcttga aagataaaat cacgaaaatc caggggctcg gaattgcaac tgcaattacc
     1201 ggaattgtcg ttacaggctt ctagagtgga ttttcgtaga cgtaaaaaat gtaatcctga
     1261 gatttcgtgt ccacgcccag atcatgcaag gcgcgtagca gttgagcacg atgatcggtg
     1321 gcatgattaa caatatgaag caagacctgc cagacgatca actccttgtc ctcttcggga
     1381 tcagtgatcg gcttggagac caactggtta tcttgtaagt ttgccagata agtgcgagtg
     1441 ttctgctcaa ccacatccca caacgagcgg atggcatcac gatcgtcaac ctcagttgtt
     1501 tctggcaacg gttcagacgg ttgaacgcca cgcaactcgc tgatccagac gtcctcggca
     1561 tcaatgagat ggatgagttg ttcccgaata gagccgcgag agtaatccac tttttgagtg
     1621 aactgctcaa aagtcaacgc agcgacatgc tccaatatct tgcggttctc ggcaaagtga
     1681 tagttgaaaa aatgacgaaa ggcttctgca ttcataaagt tgctcctttt actaagtaaa
     1741 ttttattata gcgcaaatgt tctaggaatc aaaatatgac aaaaatttca acctccctca
     1801 ccatcaacgc cagccaggaa gccatctgga aagtcctctc cgatgtcgcc cactggcaag
     1861 agtggactcc gactgtgacc aaagtcgaaa tgttggatac gcctgaaata aaactcggta
     1921 atcactacaa agtgattcaa cccaaactcc aacccgttgt ttggacggtc acagaactta
     1981 attctgccaa cttcacatgg gagtcaaagt ccccgggcat gcacatggaa gcggagcatg
     2041 tggtcaagtc gatcaatgca aatcaatctg aagtcacact caccttcgcc ttcaacggct
     2101 ggcttggcaa tctcattggc agaatgtatg gaaagatgac cgaggaatac atccgaaccg
     2161 aagcgcagtc gctgaaaaag aaagcagaat ctgcttaata ggagatttga cttgggcgtt
     2221 acaaccttca aatccatagg tgaatacgca actttgcgct ttgttgaaag caaggtcttt
     2281 ggtgcttttc caacaatagt tgtacaagac tctcctgaat ttatcgtttt atacatgcca
     2341 actggcgtac ttggaaaaaa tgtcgatcac aagcccgcaa ccaacgaact attttccgct
     2401 gacaaaatcg atattataga ctttcagtgg aaaagaaccg atgtcttgat gttaattgtg
     2461 cccggtgaag cattcagcac ctacgttatg tgggatgccg gcacaaagaa cctggattgc
     2521 tggtatgtca acttacagga gcctatccgc cgtacaagca tcggctttga tacgatggat
     2581 aatatgctcg atgttgtcat cagcccagat atgaacacct ggcaatggaa agatgacgac
     2641 gagttcgaag cagcgcaaag catcggcttt tactctgccg aaaaggcccg cgcaattcgg
     2701 gctgaaggcg agaaggctgt acaactgatc acgaaggaaa ggcgcacttt atacgaaaaa
     2761 tggaagacct gggaagccaa tcctgaatgg gaattgccca tcctttcccc cgactggcaa
     2821 agaatggatc taggatagca tggccacaaa ccgtgttcta aactggcaag gctgcaacaa
     2881 cgtccgcgat ctgggcgggc tgacggcttc caacggaagc aaaacccgtt ggggcgctgt
     2941 cgtgcgcggc gatcagccag ccaaactcac cccagaaggt tggggtgagt tatacgagca
     3001 cggcatccgc accatcgtgt cgttgcgtac gtacggcttc aatgaaaagg atcaccccgt
     3061 cgttactcca ccctaccccg atatccacgt gatgtcggtc gagatcgaag atgtcaccaa
     3121 taaaaagttc gtcgaaaaat gggcaacttc cgatctttgg ggcactcctc tttattggac
     3181 cgatgcactg aatctctggg caaatcgtca cgcggacgcg ctcaaagcca ttgcccaagc
     3241 ccaacccggt ggagtcttct tccattgcat ccgcggacat gaccgcaccg ggatcgtttc
     3301 ctttttattg ctctccctgc ttggcgtctc gctgaaagac atcaccgccg attacgaact
     3361 cagcgcagac cccgtacgag ataaacttct ggcagagcgc aatacaaccg cccatgaagt
     3421 cctgcaaaaa actcacgcag gcatcaacat cgaaaactac ctcctcaacg gcggcatgac
     3481 ccaagacgat ctcgccgccc ttcgcgcaag actactgacc aacaaataat catccttcgt
     3541 aagggcgacc cgtccgggtc tgaccaatac cttgccaagc aaaatagact tgcaaaaaca
     3601 taaactattt ggcatt
//
LOCUS       DAOREF010000209         3610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16079_length_3610_cov_4.176090, whole genome shotgun sequence.
ACCESSION   DAOREF010000209 DAOREF010000000
VERSION     DAOREF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16079_length_3610_cov_4.176090"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKI33_05625"
     CDS             complement(<1..518)
                     /locus_tag="PKI33_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36517.1"
                     /translation="MASCQLGVGITLGPIIMTLGLGATETWHTGYRIVGGFQLFLAAC
                     FVLTLPMWSDNDKPATHDEPKKLTDYKTPMGETMRQPRVWMSALLFFLYVGAEGALGT
                     WIYTLLTESRGIDPTVAGFWAGSYWATFTVGRVIAGLFANRIGINKLVIGGLLGALVG
                     AVLLVWNPSSAV"
     gene            complement(467..928)
                     /locus_tag="PKI33_05630"
     CDS             complement(467..928)
                     /locus_tag="PKI33_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36518.1"
                     /translation="MSFFTKVKQYPKIGLVILSFVAFVGLGMPDGLLGVAWPSIRSGF
                     SIPLDALGMFLTALVIGYMTSSFFSGPVVTRLGVGKVLAISCALTGIALFGYTLVPVW
                     WMMVLLGSVAGLGAGAIDAGLNTYVAAHFSEGLMQWLHASWAWASPWDRSS"
     gene            complement(995..2011)
                     /locus_tag="PKI33_05635"
     CDS             complement(995..2011)
                     /locus_tag="PKI33_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNM36519.1"
                     /translation="MTTKKSTKTLDDLARLAKVSKSTVSRALNDSPLLSQETKQRIQA
                     LARKHNFSINVSARNLRVRQSHTIAFITPDCEPNFFSPESMFGFEILGGIGNELRSLG
                     YDLLIANVNPLDPASIHTYFDSVRVDGFILLTSYNSQSTIEKLIDVDAPFITWGVPMP
                     GFSYCSVSGDNIAGGMLATKHLIQNGRQRIAFLGGEPEELTVQHRYEGYEKALKENRR
                     RAVAKYVAYGDYSYASGLDAMHRLLKQAPDLDAVFVNSDLMAIAAIKVILESGRRVPK
                     DVAVVGYDDVSIALYNNLPLTTIRQNVPLAGRLLAQNLIRYIKTGEITNVTTPVELVV
                     RKSA"
     gene            complement(2085..3407)
                     /locus_tag="PKI33_05640"
     CDS             complement(2085..3407)
                     /locus_tag="PKI33_05640"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNM36520.1"
                     /translation="MTTYKFPHNFLWGAASAAYQVEGAWNEDGKGESIWDRFSHTPGK
                     INDGNTADIACDHYHRYEEDIALMRQLGLKAYRFSTAWSRVLPNGRGRINPKGLDFYD
                     RLVDHLCAANIEPLLTLYHWDLPQALQDEGGWENRNTAHAFADYTALMVKRLGDRVKY
                     WTTFNEPSVITFIGYLTGEHAPGFKDRRMAYQVVHHLMLAHGLGMQTIRAIKPDANAG
                     IVLNLWMSEPASDSAEDLAAANKFWDENETLFLDPLFKGYYPPAVYDMVGKDMPKIQD
                     GDMALIAQKMDYVGVNYYSRNVLTAKGKVEKVEGSEYTEMGWEVCAPALRRMLVKINS
                     EYNLPPIIITENGVAFKDEVSADGKVHDPRRIEYLKEHFIQTRLAMLDGVDVRGYMVW
                     SLMDNFEWGHGFSKRFGLAYTDYATQKRILKDSGEWYAQVIKTNSIKA"
BASE COUNT          935 a          946 c          840 g          889 t
ORIGIN      
        1 ttcaccgccg aggatggatt ccaaaccaac aagaccgcac cgaccaacgc cccaagcaaa
       61 ccgccgatga ccaatttatt gatgccaatt cgattggcaa acaaccccgc gatcacgcgt
      121 cccaccgtaa aggttgccca ataactgccc gcccaaaaac ctgccacagt tgggtcaatg
      181 ccgcgcgact cggtcagcag ggtatagatc catgtaccga gcgcaccttc cgcaccaaca
      241 tagaggaaga aaagcagagc gctcatccac acacgcggct ggcgcatggt ctcgcccatc
      301 ggcgttttgt aatcggtcag ctttttcggt tcatcatgcg tggcaggttt gtcgttgtcg
      361 ctccacatcg gcaaagtcaa cacaaagcaa gccgccaaaa agagttgaaa gccaccaacg
      421 attcgatagc ccgtgtgcca tgtctctgtc gcgcccaagc cgagggtcat gatgatcggt
      481 cccagggtga tgcccacgcc caactggcat gaagccattg catcagtcct tcactgaaat
      541 gcgccgcgac gtacgtattc aaacctgcat cgatcgcacc tgcacccaaa ccagccaccg
      601 agcctaacaa aaccatcatc caccacacag ggacaagggt gtacccaaat aacgcaatcc
      661 ccgtcagggc gcaactgatc gccaaaactt tacccacacc taagcgggta acaacaggtc
      721 cactaaagaa gctggatgtc atgtagccga tcaccaaagc ggttaaaaac atgccaagcg
      781 catctagcgg gatcgaaaat ccgcttcgaa tggagggcca tgccacaccc aataagccat
      841 caggcatacc caaccccaca aaggcaacga aagacaaaat gaccaaacct atttttggat
      901 attgctttac tttcgtaaaa aagctcatgt agttttattc ctctcggttt tgcaatcgat
      961 tgcagttgct tacaaaaaaa tatacggcag gaatctacgc cgatttccga actaccaatt
     1021 ccacaggagt cgttacgttc gtgatctcgc cagtctttat gtaacggatc aaattctgcg
     1081 ccaacaaacg tcccgccaat ggtacgtttt gacgaatggt tgtcagcggc aaattgttat
     1141 acagcgcaat ggacacatcg tcgtacccca cgaccgctac atctttagga acgcgccgtc
     1201 cgctttcaag aatgacctta atcgcggcaa ttgccatcag gtcgctgttc acaaaaacag
     1261 catctaaatc tggcgcctgc ttcagcaaac ggtgcatggc gtccagacca gaggcgtagg
     1321 aataatcgcc ataggcgaca tactttgcaa cagcccgcct gcgattctcc tttaaagcct
     1381 tttcatatcc ttcataacga tgttgaaccg ttaactcttc aggttctccg cccaaaaaag
     1441 cgatccgctg cctgccattc tggatcaggt gctttgtagc aagcattccg cctgcaatgt
     1501 tatctccgct aactgagcag taactaaagc cgggcatcgg aacaccccag gttatgaaag
     1561 gggcatcaac atctattagt ttttcaatag tggattggct gttatatgaa gtaagtaaaa
     1621 tgaatccatc cacacgcaca ctgtcaaaat acgtgtggat acttgcaggg tcaagtggat
     1681 taacatttgc aattaagaga tcgtatccta gagagcgcag ttcattccca attccgccta
     1741 aaatttcaaa accaaacata ctttctggag agaaaaagtt tggttcacaa tctggtgtaa
     1801 tgaaagcaat agtatgactc tgacgtacac gtaaattccg tgcagagaca ttgatgctga
     1861 aattatgttt tctggcaagc gcctgaatcc gctgtttagt ctcctgactt aaaagcggac
     1921 tgtcattcag tgcacgcgaa acggttgact tcgaaacttt cgccaaccgt gctagatcat
     1981 caagggtttt tgtgcttttt tttgtagtca cagcagctat tctttgcaat cgattgcatt
     2041 atatcgctaa tatcgatttc aacaagagtg ttttataaat cgcttcacgc ttttattgaa
     2101 tttgtcttga tgacttgcgc ataccactcg cctgaatctt tgagtatgcg cttctgagtg
     2161 gcgtagtctg tataagcaag accgaaacgt ttggagaagc catgtcccca ttcgaagtta
     2221 tccattagtg accagaccat atagccgcgc acatctacac cgtcgagcat ggcaaggcgg
     2281 gtttgaatga aatgctcttt gagatattca atacggcgcg ggtcgtggac tttgccatcg
     2341 gcgctgactt catccttgaa tgccacgccg ttctcagtga tgatgatggg cggcaggtta
     2401 tattcgctat taatcttgac caacatacgc ctcaacgccg gggcgcaaac ttcccagccc
     2461 atttcggtat attctgagcc ttcgaccttt tccacttttc cttttgcagt aagcacattg
     2521 cgcgaatagt agttgacacc aacatagtcc atcttttgag cgatcaatgc catatcaccg
     2581 tcttggattt ttggcatgtc tttgcccacc atgtcataca cggcgggagg gtaataccct
     2641 ttgaagagcg ggtcgaggaa caaggtttcg ttttcgtccc aaaacttatt agcagctgcc
     2701 aaatcttcgg cggagtcgga ggcgggttct gacatccata gattaagcac aatgccagcg
     2761 ttcgcatccg gtttgatggc gcggatggtt tgcatcccca gcccatgcgc cagcatgaga
     2821 tgatgcacga cctgatacgc catgcgccga tctttgaagc cgggcgcatg ttcgccggtg
     2881 agataaccaa tgaaggtaat gacacttggc tcgttgaagg tcgtccaata tttgacgcgg
     2941 tcaccaaggc gcttgaccat cagtgcggtg taatcagcaa acgcatgggc agtgttgcga
     3001 ttctcccagc cgccttcgtc ctgtaaggct tgcggcagat cccagtgata aagtgtgagc
     3061 agcggctcga tgttagcagc gcacaggtgg tcaacaagtc ggtcatagaa atccaagcct
     3121 ttggggttga tgcgtccgcg cccgttgggc aagacacgac tccaggctgt ggaaaagcga
     3181 tatgccttga gcccaagttg acgcatgagc gcaatatctt cttcgtagcg gtggtaatgg
     3241 tcgcaagcga tatccgcggt gttgccatcg ttgatcttgc cgggggtgtg actaaagcga
     3301 tcccaaatcg attcgccttt gccgtcttcg ttccatgcac cttctacttg ataggcagca
     3361 gaagctgcac cccagagaaa gttatgtggg aatttgtagg ttgtcatcga agctcctcac
     3421 gaaaaaagcg gaaggctgtg ttatgccttc cgcttttgac tttgaaaatt ttattttcga
     3481 acgaacttct taccggtggc gagttccaca ctcttatacg cgccgtcgta gataccgatg
     3541 ctcttgttct tgacgatgca atcgcagaac atggtgagca ggtcgtcgta gtcggtgagc
     3601 atgtcgatgg
//
LOCUS       DAOREF010000210         3610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16081_length_3610_cov_3.716456, whole genome shotgun sequence.
ACCESSION   DAOREF010000210 DAOREF010000000
VERSION     DAOREF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16081_length_3610_cov_3.716456"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PKI33_05645"
     CDS             <1..1295
                     /locus_tag="PKI33_05645"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNM36521.1"
                     /translation="RTLSAVDCAVMVIDGAKGIEPQTLKLFEVCRKRGIPIFTFINKM
                     DRPARDPLDLLDEIRKVLGMEPVPMNWPIGDGPQFVGVYDRAEDGVYLYERTERNEKI
                     APEVFVRVDDLEKQGLLSAEKVKNLKESIELLDLAGGAFDHDAVLKEKQTPVFFGSAL
                     TNFGVRMFLDSFIQFAPPPQPYLSDTGLVLPEDTEFTGFIFKIQANMNPKHRDSVAFL
                     RICSGKFERGMDLLHAPSGKTLKLMRPYKAFANEREIIEEAYPGDVLGLPNNGIFSIG
                     DTLCTGKMVQFAPIPRFQPEHFALLRNVDVSKQKQFAKGLQQLESEGAVQILYNVNAF
                     KREPILAVVGQLQFDVVQARLESEYNVKTELERLNHSHLRWVLGEDKDIEALANRGDA
                     ILARDSRNAWAMLFQTPFLLKYYSEKNPNLKFVEITEL"
     gene            1364..2092
                     /locus_tag="PKI33_05650"
     CDS             1364..2092
                     /locus_tag="PKI33_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36522.1"
                     /translation="MDKTSFERLIHENWATYFGCSVETTEQPGTTLLPESKYEGDKVI
                     ALWHIGKHSFVQLDPAYYSQLEDLIKKLPHHTSLTGEQIEAAWGKDAISSRDVGFMHY
                     LFPSDLPHYQPSLPFILRQLTEADADLMSALQAANSPEDVDEAFVEVTHEIAFGCFHG
                     EELVAAASGYERTGFLDLGVLTHPDYRKKGLGKAVVGATCNWANQKGIIAQYRHNALN
                     TSSQNVATSLNFQKHFSSESVFLR"
     gene            2097..3371
                     /locus_tag="PKI33_05655"
     CDS             2097..3371
                     /locus_tag="PKI33_05655"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36523.1"
                     /translation="MAYLKTLYNQYPRQFWLMILGIVISTAGGGMVWPFMLIYAGGKL
                     GLPLSAVASLISINAGVGLFASFLAGTLADRIGRKVVMVSSLVMMGVAYLLLIKAETY
                     LQFALLMGMVGLSNPLYQVGADAMLADMIPTQKRTDAYAINRIAYNASFGIGPAIGGF
                     LASTSYALAFYGASTGFFIYGIIWFFFAHETLDKTVREEKASTLAGQAQIQNEAARSD
                     GYIHVFKDKAYMAFVTLFAIGMIAPSILWILLPVYTKTNFNLPEALYGWIPTTNAFMC
                     VFLQYAVSNQTRKFKTLPVASVGMLVYALGAGSVALMTGFTGFWLSMVILTFGELILI
                     PIASKYVADIAPEDLRGRYMSLYWFGWGMSRTLAPLIGGYLNDNISPRAIWIGGLLFG
                     LTSTLGLILLNSLTNTQTALQTRPKIDTTHGD"
     gene            3374..>3610
                     /locus_tag="PKI33_05660"
     CDS             3374..>3610
                     /locus_tag="PKI33_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM36524.1"
                     /translation="MKQTLALISYVVRDYDEAIAFFTNAMQFDLVEDTPQENKRWVVV
                     RPRGGVGVSLLLAKADTPEQQAAIGNQTGGRVFLF"
BASE COUNT          908 a          918 c          845 g          939 t
ORIGIN      
        1 atcgcacatt atcggctgtc gactgtgcgg tgatggtcat tgatggcgcc aagggtatcg
       61 aaccacagac attgaagctc tttgaagttt gtcgcaagcg tggcattcca atctttacct
      121 tcatcaacaa aatggatcgc cccgcccgag accctctcga tttgctggac gagatccgca
      181 aagtgctggg catggaaccg gtgcccatga attggcccat cggagacggt ccgcaatttg
      241 tgggtgtgta cgaccgcgct gaagatggag tctatctcta cgaacgcacc gagcgcaacg
      301 aaaagattgc acctgaagta ttcgtccgtg tggatgattt ggaaaagcaa ggcttgcttt
      361 cagcagaaaa agtaaaaaac ctcaaagaaa gcattgaatt gctagacctc gcaggcggag
      421 catttgacca cgatgccgtg ctcaaggaaa agcagactcc ggtcttcttt ggcagtgcat
      481 tgaccaactt cggcgtgcgc atgttccttg actctttcat ccaattcgca cctccgcccc
      541 agccttacct cagcgacaca ggtttagtcc tgccagaaga cactgagttc actggcttta
      601 tctttaagat tcaagccaac atgaacccca agcatcgtga cagcgttgcc ttcttgcgca
      661 tctgttctgg aaaattcgag cgcggtatgg atttactcca tgcgccctca ggcaagactc
      721 tcaagctaat gcgcccttac aaagcgtttg cgaatgaacg cgagatcatc gaagaagcct
      781 accctggtga tgtgttgggt ctgcctaata atgggatctt ctccatcggc gatactctct
      841 gcacaggcaa aatggttcag ttcgcgccga ttccgcgctt ccaacccgag cattttgctc
      901 tgctgcgaaa tgtggatgta agcaaacaaa agcaatttgc caaaggtcta cagcagctcg
      961 aaagtgaagg cgcagtgcaa atcctgtata acgtcaacgc tttcaagcgt gagccgatcc
     1021 ttgccgtggt cggtcagctg caatttgacg tggtacaagc ccgcctcgaa tcggaataca
     1081 acgtcaagac ggagttggaa cgcctcaacc acagtcatct gcgctgggtt cttggtgaag
     1141 ataaagatat tgaagctctc gccaaccgcg gagatgccat tctggcaaga gactctcgca
     1201 acgcctgggc aatgctcttt caaaccccgt tcttgctcaa atactattct gaaaagaacc
     1261 ctaatttgaa atttgtagaa attaccgagc tttagcgtac acgctaaata ttttagggcg
     1321 ggcggagaca acttcgcccg cctttcatgt taaacttctc actatggaca agacctcttt
     1381 tgaacgactc atccacgaaa attgggcaac ctattttggc tgttcagtcg aaacaacgga
     1441 acagcccggc actacacttt tgccggaaag caaatatgag ggcgataaag tcattgccct
     1501 ttggcacatc ggaaaacaca gcttcgtgca acttgaccca gcatattatt ctcaactgga
     1561 agatttaatc aaaaagctgc cacaccatac ttcattgact ggtgaacaaa tcgaagcagc
     1621 atggggtaaa gatgccatat cttcacgaga tgttggcttc atgcattatc tctttccatc
     1681 agacctaccc cattaccagc cctcattgcc ttttatactg cgccaactga ccgaagcgga
     1741 cgcagatctt atgtctgctt tacaagcggc gaactcaccc gaagatgtcg atgaagcctt
     1801 tgtggaagtg actcacgaaa ttgcattcgg ctgttttcac ggcgaagaac ttgttgctgc
     1861 tgccagcggc tacgagcgaa caggctttct cgacctcggc gtgctgacac accctgatta
     1921 ccgtaaaaaa gggctaggca aagctgtggt tggtgcaact tgcaattggg caaaccaaaa
     1981 agggataatc gctcaatatc gtcacaacgc gcttaatacc agttcacaaa atgtagctac
     2041 gtctttgaac tttcaaaaac actttagttc agaaagcgtt tttctcaggt aacttcgtgg
     2101 catacctcaa aactttatat aaccagtatc cacgccagtt ctggttgatg atccttggga
     2161 ttgtcatcag tactgcaggc ggcggaatgg tctggccctt catgcttatc tatgccggag
     2221 gcaaactggg tcttccgctc agtgcggtgg cgtcgctaat ctctattaat gcaggtgtag
     2281 gtttattcgc atcttttctc gccggaactt tagcagaccg cattgggcgt aaagtggtga
     2341 tggtctccag cctcgttatg atgggtgtcg cctatcttct cctcatcaaa gcagagactt
     2401 accttcaatt tgcattacta atgggcatgg ttgggttgtc aaacccgctt tatcaagtgg
     2461 gtgccgatgc catgttagca gacatgattc ccactcaaaa gcgaaccgac gcctatgcca
     2521 tcaaccgtat tgcctacaat gcctcgtttg ggattggtcc agcgattggt gggtttcttg
     2581 cgtccacttc gtatgcgctc gcattttacg gggcatctac aggattcttt atctatggga
     2641 ttatctggtt tttctttgca cacgaaacgc ttgataaaac tgtccgcgaa gaaaaagctt
     2701 ccactttagc cgggcaggct cagatccaga atgaagcggc gcgatccgat gggtatatcc
     2761 acgttttcaa agacaaggca tacatggcgt tcgtcacttt atttgcaatc ggtatgatcg
     2821 cgccttctat cttatggatt cttctaccgg tatacaccaa aaccaacttc aatttacccg
     2881 aagcgctata tggttggatt ccaaccacaa acgcattcat gtgcgtgttc ttgcaatatg
     2941 ccgtttcgaa ccaaacgcgt aaattcaaaa ccctaccggt tgcaagcgtt ggcatgcttg
     3001 tgtatgcgct tggtgccggc agtgtggcgc tcatgacggg ctttacaggg ttttggctaa
     3061 gcatggtcat tctcacgttt ggcgagctaa ttctgatccc aatcgccagc aaatatgtgg
     3121 cagatatcgc gccagaagat ttacgcggaa gatatatgtc actttattgg tttggctggg
     3181 gcatgtcgcg cacacttgcg ccactgatcg gtggctatct gaatgacaac atctcaccac
     3241 gagcaatttg gattggaggt ctgctcttcg gcttgaccag caccctcggc ttgattctac
     3301 tcaacagcct gaccaacacc caaacagcgc ttcaaactcg acccaaaatt gatactaccc
     3361 atggagatta actatgaagc aaactcttgc actgatttct tacgttgtcc gtgattatga
     3421 tgaagcaatt gcctttttca ccaatgctat gcaatttgat ttggtcgaag atacgccgca
     3481 agagaataaa cgctgggtgg tcgtccgccc gcgtggcgga gttggcgtct cgctgctgct
     3541 tgccaaggct gatacccctg aacaacaagc cgctattggg aatcaaactg gcgggcgcgt
     3601 ttttctattc
//
LOCUS       DAOREF010000211         3606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16111_length_3606_cov_4.741763, whole genome shotgun sequence.
ACCESSION   DAOREF010000211 DAOREF010000000
VERSION     DAOREF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16111_length_3606_cov_4.741763"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..2210
                     /locus_tag="PKI33_05665"
     CDS             147..2210
                     /locus_tag="PKI33_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNM36525.1"
                     /translation="MTLTNPYAQRKTLRAFEKVWTKLEGFVNRFTKSDFNPLYHLGTL
                     TIFMLLILIATGAYLTMIYRPGLDVAYRTVEKIDSNWFGSLMRSVHRYASDGMIVLIV
                     LHLLKMLFSDRFWGQRWLAWTSGWIMFAMVWLTGTMGYWLIWDQRAQWMTEYMMQYLA
                     GTSGLTYVAPDLASRTFSNFVIILFLHVFLPLIGFLGIYIHSLRLSRERWWSPRWVSI
                     QTIVGLVILSLIKPVQSAMPADLSHLIPSVPMDALYLGFLPLIDSIGNWIFWGLAILI
                     GGSLFLLPWLASGRNLGPASVTDPKCTGCNICYAECPFDAIRMNDRNDSSGYHKLAII
                     NAAQCTGCGICVGACPTDAIDLKGGYSGEQVFGAVKGALSQEKKNGSPVTVLFASQRD
                     EALGGLPAELNVSGGNAPVAVATVGEKDAARVITAVLPSVSAVNIEWIKSLHNEGARD
                     VVLLAPPYDDGVFREDAHWIANRLRARPALVTKELHWLETTPGEAKSVLNFLNDLHRA
                     ETQAKKSAPVLPVVKERSKLMPSLVSALIGTLILFAVFAVALPLDVPAGMSAAQGSAI
                     RVALDLKGKISTANIPEGMTLPEGADAEKIFGGTHYPASIRLIVDGETVLEETYQPSG
                     VSGNGRISALEFISIPSGSHQVEMLLKDDDNDFRTVYSDTLVLDVSQVVVFSYDNSSD
                     TVTVR"
     gene            complement(2296..2784)
                     /locus_tag="PKI33_05670"
     CDS             complement(2296..2784)
                     /locus_tag="PKI33_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36526.1"
                     /translation="MKKYYLLLLTLALLLPTAALAEGQFDYIVIRGFGIVEDINISNP
                     ALTQDYYAFADFSKGDVNPPSDPGIGYQVVRIHAKDSKGIPYDQLHYYPDSGYVFYDG
                     IVNGYSELGTKWYAANPTVEKPFRAALAENARLTFIPLGVFIVLLIGFLVFYLKKPKQ
                     TA"
BASE COUNT          918 a          831 c          894 g          963 t
ORIGIN      
        1 ccaatgcgat gaagatgaac caatttccgc tgttggaaga aaaagaaaat taaccgcgaa
       61 gtacgcaaag agcgcgaaga aaaaagactt tcttagcgac cttcgcggtc tttgcagttc
      121 aaaacttgaa acttggaacc tgacacatga cacttaccaa cccctacgct caacgcaaga
      181 ctctccgcgc ctttgaaaaa gtttggacaa aactcgaagg ctttgtcaat cgattcacaa
      241 aatcagactt caacccgttg taccatctcg gcacgctgac catcttcatg ttgctcattc
      301 tcatcgcaac aggcgcatac ctgaccatga tctatcgtcc tggtttggat gttgcttatc
      361 gaactgtcga gaaaatagat tccaattggt tcggctcgct catgcgcagt gtccatcgtt
      421 acgcatcaga tggcatgatt gtgctcatcg ttttgcattt gctcaaaatg ttattcagcg
      481 accgcttttg gggtcaacgc tggctggcat ggacaagcgg ctggatcatg tttgcgatgg
      541 tctggctcac gggcaccatg ggctactggc tcatctggga tcaacgcgcg caatggatga
      601 ccgaatacat gatgcaatat ctggcgggca catcgggtct cacctacgtc gcgcccgatc
      661 tcgcctcgcg caccttctcc aacttcgtca tcatcctttt tctgcacgtc tttctgccac
      721 tcatcggctt ccttggcatt tacattcaca gcctgcgcct ctcgcgcgaa cgttggtggt
      781 cgccgcgctg ggtcagcatt caaaccatcg ttggacttgt gattctttcg ctaatcaaac
      841 ccgtccaatc ggcaatgccc gcagatttat ctcatctgat tccgtccgtg ccaatggacg
      901 cgctgtactt gggctttttg cctttgatcg attctattgg gaattggatt ttctggggac
      961 tggcgattct catcggtggg agtttgttcc tgctcccttg gctcgcttcg ggacgtaatc
     1021 tgggacctgc ttctgttacc gatccaaagt gtactggctg taacatttgc tacgccgaat
     1081 gtccgttcga tgccatccgc atgaatgacc gcaatgattc ttcgggctat cataaactgg
     1141 cgatcatcaa cgctgcgcaa tgcacgggct gtggaatctg cgtgggggcg tgcccaactg
     1201 atgcgattga tttgaaaggc ggttatagtg gagagcaagt ctttggcgcg gtgaagggcg
     1261 cgttgtctca ggaaaagaag aacggtagcc ccgtgacagt tttgtttgca agtcaacggg
     1321 atgaagcgct gggcggcttg cccgcagaat taaacgtcag cgggggaaat gctcctgttg
     1381 ctgtggcaac tgttggtgag aaagatgcgg cgcgcgttat cacggcggta ttgccgagcg
     1441 tgagtgcggt caacatcgag tggatcaagt ccctacacaa cgaaggcgcg cgcgatgtcg
     1501 tgttgctggc tccgccgtat gacgatggcg tgttccgcga agacgcgcat tggattgcga
     1561 accgcctgcg tgcgcgacct gcgctggtga cgaaggaatt gcactggctc gaaacgacgc
     1621 caggtgaagc gaaatcggtg ttgaattttc tcaacgattt gcaccgtgct gaaacgcagg
     1681 cgaagaagtc cgcgcctgtg ttgcctgttg tgaaggaacg cagcaagttg atgccctcgc
     1741 tggtgagcgc gttgatcggc acgctgattt tgtttgcggt gtttgcggtt gcgcttccgt
     1801 tggacgtccc cgctgggatg tctgcggcgc aaggctctgc catccgcgtg gcgttggatt
     1861 tgaaagggaa aatttccact gccaatattc ccgaaggcat gaccctgccc gaaggcgcag
     1921 acgcggaaaa gattttcggc ggcacgcatt accctgcttc cattcgtttg attgttgatg
     1981 gtgaaaccgt gttggaagaa acctatcaac catcgggtgt cagcggcaac gggcgcatct
     2041 ctgcgctgga atttatatcc attccatctg gctcgcatca agttgaaatg ttgctcaagg
     2101 atgacgataa cgatttccgc acggtttatt cagatacgct cgtgctggat gtttcgcagg
     2161 tggttgtgtt tagttatgac aatagcagtg atacagtgac ggtgagataa agaatcttga
     2221 ccacagagac cacagggttc acagagaata tttttaaatc tccgtgttca ctgtggtctc
     2281 tgtgatgata ttgttttatg cggtttgttt gggcttcttc agatagaaca cgaggaaacc
     2341 aattagcaag acaataaaca cgccaagcgg aataaaggtc aaccgtgcgt tttcagccaa
     2401 tgcagcacgg aaaggttttt caactgtggg gttggctgca taccattttg tacccaattc
     2461 agaatagcca ttcacaatgc cgtcataaaa gacatatccg ctatcggggt aataatgtaa
     2521 ctgatcatac ggaatgccct tgctatcttt ggcatgtatg cgtactactt gataaccgat
     2581 cccagggtct gagggcgggt tgacatctcc ctttgaaaaa tcggcaaagg cgtaatagtc
     2641 ctgtgtcagc gctgggttgg agatgttgat atcttcaaca attccaaacc cgcgaatgac
     2701 gatatagtca aattgtccct cggcaagggc ggcagtgggg agcaacaatg ccagagttag
     2761 caagagaagg taatattttt tcataagtct ccaaggttgg atggtaggtt gtaagtaagc
     2821 caaactgctt actaatattc gtgtttgatc cttttttgta cagggaattg ggttatggtt
     2881 cggcttcatt tcacatcaaa gtaattttat aggtaaaaaa agaactaatt ccaatgaaaa
     2941 aagaatgatt aataataaat gattgatcgt acctgggttt ttataaaata cttccagaac
     3001 taacagcctc caatcacaac cacatacctt agacaggact ttatggaatg gaaatggttt
     3061 aaacgaaaac gcacctatga atagaggcgt tttcgttttt gcttatctgg gggcatattc
     3121 caggcggcgc gaaatttacc catatacatg atatatgcca ctctggacag gtccgacttt
     3181 ttgcagaaaa ggtagtttca atccactcag tagaggtggg ggatatttca aagtaaaaag
     3241 agtttcaacc tctcaaagcg aaaaacatac catgtggatg aacgtcacac aagtattgcg
     3301 caaatgcatc aagcgccgaa agggataggt cataattcag actgcaaaag aacttcaaat
     3361 ccatgttagg cctgtatctc tagaccgttt tattcacggc acaagacgta attctatatc
     3421 gggcaggttg gcaacctgaa attctttgct gccaacctat actcattatg attattttga
     3481 attattgcca aacagaaaat caacatcaac atcaacatca acatcaacag aaacaaaacc
     3541 ttaagtgttt cattcgatct gtcgctgata agttttacat tttttggaac aagcagatgc
     3601 atatct
//
LOCUS       DAOREF010000212         3605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16119_length_3605_cov_3.531268, whole genome shotgun sequence.
ACCESSION   DAOREF010000212 DAOREF010000000
VERSION     DAOREF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16119_length_3605_cov_3.531268"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKI33_05675"
     CDS             <1..385
                     /locus_tag="PKI33_05675"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNM36527.1"
                     /translation="PIWYDTLLINWFAWTGVLLGVFSLFMMHDIVRRVFGRFIGWLFV
                     LTVSTLCGVGIYIGRFLRWNSWDVILHPYERMREMLYYVLHPSMQSILFISVFSALFV
                     FIYITTYTFGLLFQEQAQPISQDIS"
     gene            382..1179
                     /gene="yqeB"
                     /locus_tag="PKI33_05680"
     CDS             382..1179
                     /gene="yqeB"
                     /locus_tag="PKI33_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HNM36528.1"
                     /translation="MNPLILIRGGGDLASGVALRLHRVGFQVAILELDKPLAVRRTVS
                     FSEAVYEGSQTVESVTARLISADQFQVTLEAGEIPVLIDPHANILNNQFLTSPTSTFV
                     VDARLMKTEPEPLPERVALHIGLGPGFTAGANCHAVIETMRGHALGRVYWDGQTAPDS
                     RQPDGDTRRVLRAPAAGTLQPNKAIGAFCKEGETLAFVSGKPVISPFDGILRGLIHPK
                     VELTEGMKIGDVDARNEPSMVRFVSDKALSVAGGVLEAVYVKLREME"
     gene            complement(1256..1981)
                     /locus_tag="PKI33_05685"
     CDS             complement(1256..1981)
                     /locus_tag="PKI33_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36529.1"
                     /translation="MKSNILVVDDEPVARQSLTDILKLEGFIVASVPNGQAAVEHIRT
                     HTVDLMIVDLKMPGMDGLEVIQVVNQTSPDTEIILLTAHGSIETAIQALRLRIHDYLL
                     KPASPAQILASVKKGLSRRDRSRVSNAAVTGSESTQDIYTLKDGTMVDLSRRQIKFKS
                     KIEHLTPAEGRLLRILMENEGKVFSHRELVLLVQGYDTSAREAPEILRPLVSRLRHKL
                     EAFPSLSEKVVSVRGTGYLYEGG"
     gene            complement(1978..>3605)
                     /locus_tag="PKI33_05690"
     CDS             complement(1978..>3605)
                     /locus_tag="PKI33_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36530.1"
                     /translation="KDFVTLLLEWHKTNMEAPFVYVVDDEPGIAMLCERVLSRAGYRV
                     ATETNPRKAVEYFEDHSIDLLLVDIRMPEVDGFEVIQHVQRWQPDAAILIMTGHGTVD
                     TAIRALRQGVDGLILKPFKQGAELVDAVKLAFADNQKKRDAARTQALRPLFTVTEAML
                     SEIRRAPLLDLIIKAITAQVNCSQAACYQRQNEAKEFTRVASKGTPDTMPLLDLILHA
                     DASDSPMLINTTGPGDQTLKALLADLEVGSAILVPVHHPSLSTVFYAARGVNEPAFRE
                     SDLEMFQILARQAATALENSRLYAEQLEYVRQIETSQKALIQAEKMAAVGRLSASLAH
                     ELNNPLQGVQNCLHLAGREDLPEEKRKEYFDLARAELERLMNTARRMLDFYRPNAATQ
                     TQVNLVDLLEYVLSLMSKQLHEAHVNVIKDIRDDLPLITAVGSQVQQVFINLILNAAD
                     AMSPAGGDLRITARTWEGGVELLFQDSGTGIPAERQPNIFEPFFSTKDGGTGLGLTVS
                     YNIITAHGGSLELLPDRAPGACFRILLPTGGKQ"
BASE COUNT          818 a          921 c          977 g          889 t
ORIGIN      
        1 gcccatttgg tacgacacct tactcatcaa ctggttcgca tggaccggag tcttgttggg
       61 tgtcttttcg cttttcatga tgcacgacat cgtacggcgt gtgtttgggc gtttcatcgg
      121 ttggttattc gtgctcacgg tcagcacact ttgcggggtg ggcatttaca tcgggcgctt
      181 cttgcgctgg aattcatggg atgtcattct gcatccttat gaacgcatgc gcgagatgct
      241 atactatgtc ttgcatccaa gcatgcaatc gattcttttt attagtgtct tctcggcttt
      301 gtttgttttc atctacatta ccacttatac attcggttta ttgtttcaag aacaagccca
      361 acccatttca caggatattt catgaatcca ctcattctca ttcgcggtgg cggtgacctc
      421 gcttcaggcg tggcgctccg cttgcaccgc gttggttttc aggttgccat tctcgaactg
      481 gataaaccct tggcagttcg tagaacggtc tcattttcag aggcggttta tgaaggctca
      541 caaacggttg aaagcgtaac cgcaaggctt atttcggcag accagtttca ggtcacgtta
      601 gaggcgggcg aaattcctgt gttgatcgac ccacatgcca acatcctcaa caatcaattt
      661 ctgacgagtc caacttccac attcgttgtg gatgcgcgtt tgatgaaaac ggagccagag
      721 ccattgccag agcgtgtggc gcttcatatc gggctgggac ctgggtttac agccggagcc
      781 aattgccacg ctgtgatcga aaccatgcgc ggacatgccc tcggcagagt gtattgggat
      841 gggcaaaccg cgcccgatag ccgccaaccc gatggtgata cccgccgtgt actgcgcgcc
      901 cctgccgcag ggacgttgca acccaataaa gccattggtg cgttttgcaa agaaggagaa
      961 accctcgcct ttgtaagcgg caagccggtt atcagcccat tcgatggcat cttgcgcgga
     1021 ctcattcacc ccaaagtgga gttgaccgaa ggcatgaaga tcggcgatgt agatgcacgc
     1081 aatgagccaa gcatggtccg tttcgtttca gataaagcct tgtccgttgc aggcggcgtg
     1141 ttggaagcgg tgtatgtgaa gttgcgcgag atggaataac taacaatcta ttagcatgta
     1201 ctaaaaaagg acagcccatg agctgtcctt tttttatttt ctaatctact aagccctacc
     1261 caccctcata caaatacccc gtcccgcgca cgctaacgac tttctccgaa agtgagggaa
     1321 aggcttccag tttgtggcgc aggcggctga caagcgggcg caaaatttcg ggggcttcgc
     1381 gcgccgaggt gtcgtagccc tgcacaagca aaaccaactc acgatgcgaa aagaccttgc
     1441 cttcgttttc catcaggatg cgcaacaggc gtccttcggc gggtgtgaga tgctcgatct
     1501 tgctcttgaa cttgatctga cggcgcgaga ggtctaccat ggtgccgtct ttgagggtgt
     1561 aaatatcttg ggtggattca gagccggtca ccgccgcatt gctgacgcgg gaacggtcac
     1621 gtcggctcaa gcctttcttt acgctggcaa ggatctgtgc cggcgaagcg ggctttagca
     1681 aataatcgtg aatgcgcaaa cgcaaggctt gaatggcagt ttcaatcgag ccatgcgccg
     1741 tgagcaaaat aatttcagta tctggggaag tttggttgac gacttgaata acttcaagcc
     1801 cgtccatgcc gggcattttg agatccacga tcatcaagtc cacggtatgg gtgcggatgt
     1861 gctcgacagc ggcttgccca tttgggacag aagccacgat gaaaccttcc agtttcagaa
     1921 tatcggtgag agattgccgg gctacaggtt cgtcatcaac gactaggatg ttcgacttca
     1981 ttgtttacct ccggttggca gtagtattcg gaaacaagcg ccgggtgcgc gatctggaag
     2041 cagttccagc gagcctccat gtgctgtaat tatattgtaa cttaccgtta aacctagccc
     2101 tgtccctcca tctttagtac taaagaaagg ctcaaaaatg ttaggttggc gttcggcagg
     2161 gatgccagtg ccgctatcct gaaaaaggag ctccacaccg ccttcccatg tgcgggcggt
     2221 gatgcgtagg tcgcctccgg ctggagacat ggcgtcggcg gcgttgagga tgaggttgat
     2281 gaacacctgc tggacctggc tgcccacggc ggtaatgagc ggcaggtcat cgcgaatgtc
     2341 tttgatgacg ttcacatgcg cctcatgcaa ctgtttggac atgagactga gcacatattc
     2401 aagcaaatcc accaagttga cttgggtctg ggtggcggca ttcgggcggt aaaagtcgag
     2461 catgcggcga gccgtgttca tcaggcgctc aagctccgcc cgggctaggt caaaatactc
     2521 tttgcgtttt tcttcgggca ggtcttcgcg cccggcaaga tgcaaacagt tttgcacgcc
     2581 ttgtaaggga ttattcaact catgcgcaag agaggcgctt aaacgtccca ccgccgccat
     2641 cttctctgct tggatcaagg ctttttgtga agtttcgatc tgacgcacat attccaattg
     2701 ctcggcatac aagcgcgaat tctccagtgc ggtcgcggct tgacgggcaa ggatctggaa
     2761 catctccaaa tctgactcgc ggaaggctgg ttcgttcact ccgcgcgcgg cgtagaagac
     2821 cgtgctcagg ctggggtgat gaaccggcac gagaatcgct gaacccactt cgaggtccgc
     2881 aagaagcgct ttgagggttt ggtcacccgg acccgtggta ttgatcagca tcggcgaatc
     2941 ggaagcatca gcatgaagaa tcaaatccaa caaaggcatg gtatctggcg tgcctttact
     3001 ggcaacacgg gtgaattctt tggcttcatt ctggcgttga taacaagccg cctgcgaaca
     3061 attgacctgt gccgtaatgg ctttgatgat aaggtcgagc aagggcgcgc gccgaatttc
     3121 agaaagcatg gcttcggtaa ctgtaaagag cggacgcaac gcctgcgtgc gggcggcatc
     3181 gcgcttcttt tggttatcgg caaacgccag tttgacggca tcgaccaatt ctgcgccttg
     3241 cttgaacggt ttgagaataa gaccatccac gccttggcga agggcgcgaa tggcagtatc
     3301 cactgtgcca tgacctgtca tgatcagaat ggcggcatcg ggctgccaac gttgcacatg
     3361 ttgaatgact tcaaagccat ccacttcggg catgcgaata tcaaccagca aaaggtcaat
     3421 agaatgatct tcaaaatatt ccaccgcctt gcgcgggttg gtctctgtcg ctacgcgata
     3481 gccggcacgc gaaagcaccc gctcgcaaag catggctatg ccgggttcat catcaacaac
     3541 gtaaacaaaa ggtgcttcca tgtttgtttt gtgccactct aagagtagag taacaaaatc
     3601 ctttg
//
LOCUS       DAOREF010000213         3597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16199_length_3597_cov_3.588650, whole genome shotgun sequence.
ACCESSION   DAOREF010000213 DAOREF010000000
VERSION     DAOREF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16199_length_3597_cov_3.588650"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PKI33_05695"
     CDS             complement(<1..1246)
                     /locus_tag="PKI33_05695"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNM36531.1"
                     /translation="MDRQPHRKQKPEFFEPLKIFFAILIVSLVFFAPIVRSEKAETPN
                     AIQEYPALNPFASSADACPWNCIKCTEWDPDAWPKTCLTYECTDASGNCGDPGDGGGG
                     GYPPPTISHVLTCSNTGNNGWCIGTLSLDLTAADPQGQSVVISGTINGDSFACPSGQT
                     TCSIPLPEASGTVTYKVDSATGLSANGSTSYHLDVTTPQISGDINGSSGTNGWFISQT
                     SVTASASDSLSGLASFEVNVNNTGYASYSDTTFTDGTHTIQFRATDNAGNVTETAQQS
                     INVDTTAPTLDLSTTGTIGQNGWYVSAVILTPTASDTTSGLYSFEATTDGVTWTSVSA
                     PITLTDGIYTVQFRATDNAGNVSQTPSQQIKVDATTPNLSLDINGIRGQNDWYISSVA
                     VTPNASDGGSGINKVEASVDNGS"
     gene            complement(1382..1813)
                     /locus_tag="PKI33_05700"
     CDS             complement(1382..1813)
                     /locus_tag="PKI33_05700"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNM36532.1"
                     /translation="MAWITEQVLLAGAGINENNWKEVIDLGVTAVVNLRAENQDNFGA
                     PLPFAYLWLPTEDHTDPNPYQLLVGAQMIDTLVKSGHKVLVHCKMGIHRSATMVVAFL
                     IYSGMDKEEAIWHFKKNGSRLYGSEENHQTLDKFVKLISEK"
     gene            complement(1895..2641)
                     /locus_tag="PKI33_05705"
     CDS             complement(1895..2641)
                     /locus_tag="PKI33_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNM36533.1"
                     /translation="MTYNILDGGENRVAHILKVIQSANPDVVILQEVYTERFLQSLSQ
                     ESGMKYYIGTGNKQRKVALLSKLPVRSFKSHHPIFPIWRNFIDAEIEIAQFGTARIIG
                     VHPMANLGIIFEIWRFLEASFIVRHLQKTNGQPCLIAGDFNAIAPGEKVNTQSMPTWL
                     KWMIYLQGNRVYHSSIAKYLSAGYIDCFRLMNSDDGFTLPPPKPNARLDYIFVNTPMK
                     SFLKRCWVVREPESVNHASDHYPVMAEFDF"
     gene            complement(2774..3130)
                     /locus_tag="PKI33_05710"
     CDS             complement(2774..3130)
                     /locus_tag="PKI33_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36534.1"
                     /translation="MDILIAIDNDTDLSALATAARRLKGIVQGKNKGADIFLANPNGE
                     YIGRVCHWKGCGPQFRSTCDAWHCGVRPYLHDDFGDIRLNSHLVKEPPLEIWPTLIYR
                     QKVADDLLPFLSKYEV"
BASE COUNT          947 a          797 c          826 g         1027 t
ORIGIN      
        1 acgatccgtt atcaacggac gcttcaacct tgtttatgcc tgatcccccg tctgaagcat
       61 tgggtgtcac tgcgaccgac gagatgtacc agtcattctg tccacggatg ccgttgatgt
      121 ccaggctcag gttgggggtg gtggcatcca ctttgatctg ttggcttggg gtttgtgaga
      181 cattgcctgc attgtccgtg gcacggaatt ggacagtgta gatgccatct gtcagagtga
      241 ttggggcact tacgctggtc caagtgactc catcagtggt tgcttcgaag ctatatagac
      301 cagaggtggt atcactggcg gttggagtca gtatcaccgc agagacatac cagccgtttt
      361 gtccaattgt tcccgtagta gagaggtcga gggttggtgc agttgtatcc acgttgattg
      421 attgctgtgc cgtttcggtc acattacccg cattgtctgt ggcgcgaaat tgaatggtgt
      481 gtgtgccgtc ggtaaaagta gtgtcgctgt aacttgcgta accggtgttg ttcacgttca
      541 cttcaaagga tgcgagtccg gagagggaat ccgaagccga ggcggtgaca gaggtttgag
      601 agatgaacca accgttcgtc ccggaggagc cgttgatgtc tccgctgatc tgcggggtgg
      661 tcacatccaa gtgataggat gtagagccgt tggcagagag tccagtagct gaatccactt
      721 tgtaggtgac ggtacccgat gcttcaggta atgggatcga gcaggtggtt tgtccacttg
      781 ggcaggcgaa ggaatcacca ttgatcgttc cactgataac aacggattgc ccttgcgggt
      841 ctgcggcagt gagatccaaa ctgagggtgc caatgcacca accgttgttg ccagtgttgg
      901 agcaagtgag gacgtgtgag attgtgggcg gtgggtatcc accgccacca ccgtctccgg
      961 ggtcgccaca gttgccgctt gcatccgtac actcgtaggt gaggcaggtt tttggccatg
     1021 catctggatc ccattcggta catttgatac agttccaagg acaggcatct gcgcttgatg
     1081 cgaaggggtt gagagctgga tactcttgga tagcatttgg agtttctgct ttttctgatc
     1141 gaacaatggg cgcgaaaaag acaagcgaaa cgatcaggat ggcgaaaaag atctttagag
     1201 gctcgaagaa ttcgggtttc tgtttcctat ggggttgacg atccattgcc ccctcctatg
     1261 ggttttattt aataggattg tacgatgatt ccctccccca tagatttacg tttttgttag
     1321 gaaagatcga tactgtagat tatagagtca ctccccagga tgcgtttgct ttttaccaaa
     1381 ttcatttttc tgaaataagt ttgacaaact tatccagcgt ttgatgattt tcttctgatc
     1441 cataaagccg agaaccattt ttcttgaaat gccaaattgc ttcttcttta tccatgcctg
     1501 aataaatcaa gaaagcaaca accatcgttg cggagcgatg gattcccatt ttgcaatgaa
     1561 ctaaaacttt gtgcccactc ttgaccaagg tgtcaatcat ttgagcgcca acaagcaatt
     1621 gataaggatt gggatcagta tgatcttccg taggtaacca caaataagca aacgggagag
     1681 gagccccaaa attatcttga ttttctgctc gcaaatttac cacggcagtt acacctaaat
     1741 ctataacttc tttccagtta ttttcattga tgcctgcgcc agcgagtaaa acttgctctg
     1801 ttatccatgc cattttttgc ctgatcctga atgtataaac ggaaatttgt gttcgcagac
     1861 tgcaattgaa ctatttgcta tcacaagaca taccctaaaa atcaaactca gccataacag
     1921 gataatgatc tgaagcatga tttacgcttt ctggctcgcg cactacccaa catcttttca
     1981 aaaagctttt cattggtgtg ttcacaaaaa tgtaatctaa ccgcgcatta ggctttggcg
     2041 gggggagcgt aaacccatcg tcagaattca tcaaccgaaa acaatcaata tatcctgctg
     2101 acaagtactt tgctattgaa gaatgataaa cccgatttcc ttgtagatag atcatccact
     2161 ttagccaggt gggcatactt tgagtattga ccttttcgcc tggggcaatt gcattgaaat
     2221 ccccagcgat taggcaaggt tgcccatttg ttttttggag atgcctgaca ataaaacttg
     2281 cttccaagaa acgccaaatt tcaaaaataa tcccaaggtt agccattggg tgaacaccaa
     2341 taattcttgc agttccaaat tgcgcaatct caatttcagc gtcaatgaaa tttcgccaaa
     2401 tcggaaaaat aggatggtga cttttgaaac tacgaacagg caacttgctg agtaatgcca
     2461 cttttcgttg tttattacct gttccaatat aatatttcat acccgattcc tgtgacaagg
     2521 actgtaaaaa cctttcggta tagacctctt gcaggataac aacatctgga ttggcggatt
     2581 ggattacctt caaaatatgt gctactcgat tttcaccacc gtcaagaatg ttgtatgtca
     2641 ttacacggaa aagcatatat attctgttcc tatagatcta ctttagggcg tttagtggtt
     2701 tgttgggaga aacagcattg tcatccaact agataatgca tgagattccc ataagcccat
     2761 attggatatg gatttacacc tcatacttgg ataaaaaggg taaaagatca tcggcaacct
     2821 tttgacgata aattaaagtt ggccatattt caagcggcgg ttccttcacc aaatgtgaat
     2881 tgagtcgaat gtcaccgaaa tcatcatgca aataagggcg caccccacag tgccatgcat
     2941 cgcaggtgga acgaaactga ggtccacaac ctttccaatg acaaactcga ccgatatact
     3001 caccattagg attggctaga aaaatatcgg cgcccttatt cttcccttgt actattcctt
     3061 taagtcgtcg agccgctgtt gccaatgcgg acaaatctgt gtcattatca attgcaataa
     3121 ggatatccac atctttcgga tctgctatgg gagttacgat tgatcctagc aatgcaaccc
     3181 gttttactcc cggaaggcta ataacacgat cgataaaccg aaaagattct gtgatgagaa
     3241 aagctcgaac ttcttgactg ttctaaatca ttacgacctt taccagatat gttcattcat
     3301 tttatcgctc aacagtttgc gcccacccct tcttatatgt tgttcagcgg atgtggcaaa
     3361 gccgtccaac cagcctgtcc tggacggaca gtccagagaa aaaggcttgg gatgtacgca
     3421 gactccccag cgtcctcctg tggacgtttt gttagactgc gtttggtgca tgatacaaag
     3481 ggatgtatgc gtgacgattt tcaacggctt tgctatcttt gcacattatt accaatgcct
     3541 atgtaaaccc tcagttttcc gaaaataaaa atttgtaaac ttaattagcc ttgcctt
//
LOCUS       DAOREF010000214         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16218_length_3594_cov_4.797118, whole genome shotgun sequence.
ACCESSION   DAOREF010000214 DAOREF010000000
VERSION     DAOREF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16218_length_3594_cov_4.797118"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2323)
                     /locus_tag="PKI33_05715"
     CDS             complement(29..2323)
                     /locus_tag="PKI33_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36535.1"
                     /translation="MKTGSNLFAPDKNTPPKGPTSWAKLSYFQKFAIITMVFALPVLA
                     FIPLAYEQYQRIDQYGTKEAQGVLYLRNLWDLSDSLHNYVAVSTNVENGVADKTDLTR
                     AKNNVTDALNALKENTEIEKNLGLSITAQNLEDAWKNSQDINNTLQLNVTVSNVIREV
                     GDKSFLILDPDLDTYYLMDAVLLRLPENQNNLYTIERLTQKIDSGSALTPEEYQQLQN
                     DVNIIQNNLEDITKNLKVVAENNKNNVFSLALTESLDSHKSNFDTYSKEVLSYTSANS
                     SGTSSQASTLFTEVINTEAVFYNAASSALEQGIQSRVSSYSFSIEFYSIVSILSILVA
                     FTLGNQLLRSIIVPLQKTIEASEKFAAGEFHTRIDYTTADEAGLIIQAFNKLAEEIDV
                     KQNNLQLRSNALENKTSELETIAKVSREITSFRDLSSLLTAATNLIHENFGYYHVGIF
                     LLDERKEFAVLAASNSEGGKRMLERGHHLKVGETGIVGYVSQTLEARIALDVGKDAVF
                     FNNPDLPETRSELALPLVVNTQILGVLDVQSTQPEAFDQEDVKTLNILADQLAIAIQN
                     ANLFSEAEKAIEATRATYGQLSREAWNRILRSQAKVGFLATPPSTVQIQNETLESNIA
                     KAIETGDLIHDADGLTISVPVKIRGQIIGAIRLKKSDISEAWTQDETNLAIALSDQLS
                     GALESARLYRESQQRAYRESLVSDISARINASATRDLIIRETVQELGQSLVNTSVTFQ
                     LLDQSFSENPNNGNGRTAKSSRNE"
     gene            complement(2357..>3594)
                     /locus_tag="PKI33_05720"
     CDS             complement(2357..>3594)
                     /locus_tag="PKI33_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36536.1"
                     /translation="LTQLGIFQLSAPDVPKTNIESWVVFVTDYIAIGVVIVVAANMLK
                     RAFSRVADQMQKTFETLETERQNLENKVQERTVELETRMAQLRASTLTARAIAETQDI
                     AELLKNAANLIAERFEYYHVGIFILDDLKRNAYLQASSSEVGNSLIGQIFRLDTDRKN
                     PLTTAIEGNRTIIITDLDRSNFVRDPNFPLTRSRMIMPLSVRGKEIGVIDIHSDQPNA
                     FTSEDAEILQTLADLTAISFDNVRLLAETRNLLTQLETNTTLQTQRTWTKFTSRQKSA
                     YQYTPAGVRPIFSQDKNNESDGLYTPIILHGQEIGRIKLKKRKGHLDEWTDRERDLVE
                     KISSQVALALENSRLVDEAQKNALRNQMIASFSSYVRETLDVDAVIQTAATELRKVFD
                     LKEAEIFIGASTTKQTDES"
BASE COUNT          967 a          696 c          788 g         1143 t
ORIGIN      
        1 cgtaaactca tgttattcat aagtgacgtt actcattcct ggaactcttt gcggttcttc
       61 catttccatt gtttggattc tcgctaaaag actgatctaa caattggaat gtgaccgaag
      121 tatttacaag cgactgacct aattcttgca ctgtttcgcg gatgattaga tctcttgtcg
      181 cagaagcatt gatacgagct gaaatgtctg aaactagcga ctcgcgatag gcgcgttgtt
      241 gcgattcgcg ataaagacgg gcactttcta acgcaccgct caattgatct gaaagagcaa
      301 ttgcaagatt ggtctcgtct tgtgtccatg cttcggagat atctgatttc tttagccgaa
      361 tggcgccaat gatttgtcca cggatcttga ccggaacgct gattgttagc ccatcagcat
      421 catgaataag gtctccggtt tcgattgctt tggcaatgtt agattccaga gtctcgtttt
      481 ggatctgaac cgtagatggg ggggttgcga ggaagccaac cttggcttga cttcgtaaaa
      541 tacgattcca cgcctcacgg ctcaattgac cgtatgtagc acgtgttgcc tcaatcgcct
      601 tttctgcttc actaaaaagg ttggcgtttt gaattgcaat tgcaagctga tcggcaagga
      661 tatttaatgt ctttacatct tcttggtcaa acgcttcagg ttgagtgctt tgtacatcca
      721 atacaccgag aatctgagtg tttactacaa gaggtagcgc tagttcagaa cgggtctcag
      781 gtaagtcagg gttgttgaag aaaacagcat ccttacctac atcaagagca atacgggctt
      841 caagtgtctg tgatacatag ccaacaatgc cagtttcgcc tactttgagg tgatgaccgc
      901 gttcgagcat tcttttaccg ccctcactgt tagaggctgc caaaacagca aactctttgc
      961 gttcatccag taaaaaaata cctacatgat aatatccaaa attctcgtgg atgagattag
     1021 tagcagctgt aagcagagat gatagatcgc ggaaggatgt aatttcgcgc gagacttttg
     1081 caatagtttc aagctcgctt gtcttgtttt ccaaggcatt gctgcggagt tggaggttat
     1141 tttgtttgac gtcaatttct tctgccaact tgttgaaggc ttgaatgatt aatccggctt
     1201 cgtctgcggt agtgtaatca atgcgagtat gaaattctcc ggcagcaaac ttctctgaag
     1261 cttcaattgt tttctgaaga ggtacgatga ttgagcgtaa taattgattt ccgagtgtaa
     1321 aagccaccaa aatgcttaat atagagacaa tggagtaaaa ctcaattgag aagctataag
     1381 agcttacacg gctttgaatg ccttgctcca gagcagaaga tgcagcatta tagaaaacag
     1441 cttctgtatt tataacttcg gtaaatagtg tgcttgcctg tgatgatgtt ccgctactat
     1501 tcgctgacgt gtaagataaa acctctttag agtatgtgtc aaaatttgat ttatgtgaat
     1561 ccaaactctc ggtcaacgct aacgagaaaa cattgttttt attgttttca gccactactt
     1621 ttagattttt agtaatatct tccaggttat tttgaataat gttgacatca ttttgcagct
     1681 gttgatattc ttcaggcgtt agagcgcttc cagagtcaat tttttgtgtg agtctctcaa
     1741 ttgtatatag attgttttgg ttttctggaa gccgcaacag aacggcatcc ataagatagt
     1801 aagtatccaa gtccggatcg agaatcaaaa aagatttatc gccaacttct ctaataacat
     1861 ttgagacagt aacatttaat tgtagggtgt tgttgatgtc ttgtgaattt ttccaagcat
     1921 cttctaaatt ttgtgcagta atgcttaatc caagattttt ttcaatctct gtgttctctt
     1981 taagggcatt tagcgcgtca gttacgttat tttttgccct tgttaagtca gttttatcgg
     2041 caaccccatt ctctacattg gtacttactg cgacatagtt atgaagactg tctgaaagat
     2101 cccaaagatt acgtaaatac agaacgcctt gagcttcttt tgttccatat tggtctatgc
     2161 gttgatattg ttcatatgca agcggaataa atgcaaggac tggcaatgca aaaaccatcg
     2221 tgataatggc gaatttttgg aaatagctta atttcgccca agaggttggt cccttaggag
     2281 gggtgttttt atctggagcg aagaggtttg agcctgtttt cataaagacc ttagtaaaga
     2341 aacaagtttc tacaaattag ctttcatctg tttgcttagt agttgacgct ccgataaaaa
     2401 tctctgcttc cttgaggtca aagacttttc gtaattcggt agcagctgtt tgaattacag
     2461 catctacatc caatgtttcg cggacatagc ttgagaagct tgcaatcatt tggtttcgta
     2521 aagcgttctt ttgagcctca tctacgagac gactgttttc aagagcaaga gccacctgag
     2581 aagatatttt ctcaaccaga tctcgttcgc gatccgtcca ttcatcgaga tgcccttttc
     2641 tcttcttgag cttaattctg ccgatctctt gtccgtgcaa aatgatggga gtgtataagc
     2701 catcactctc gttgtttttg tcttgagaaa aaattgggcg aacacctgct ggtgtgtatt
     2761 ggtaagcgct cttttgacgg cttgtaaact tggtccaagt tctttgagtc tggagtgtgg
     2821 tgttagtctc aagctgagtg agcaagtttc tagtctccgc aaggaggcgg acgttatcaa
     2881 atgagatcgc agtcaagtct gcgagagttt gtaaaatttc agcgtcttct gatgtaaagg
     2941 cattgggttg atcagaatga atatcgatca ctccaatttc tttaccacgt acagacaatg
     3001 gcataatcat acgtgagcgg gtaagaggga aattcggatc gcgaacaaaa tttgaccgat
     3061 cgaggtcagt aatgatgata gttctattgc cttcgatggc ggtcgtgaga ggattttttc
     3121 ggtctgtatc aagacggaat atttgaccaa ttaaagaatt tccaacttca gaagaagaag
     3181 cctgaagata ggcatttcgt ttcagatcat caaggatgaa aattccaacg tggtaatact
     3241 cgaaacgttc agcgattaaa tttgcagcat tttttaacaa ttcggcaata tcttgtgttt
     3301 cagcaattgc acgagcagtt agggtagaag cgcgtagttg tgccatgcga gtttctaact
     3361 caactgtacg ttcttgaact ttgttttcga ggttttggcg ctctgtttcc agcgtttcga
     3421 aggtcttttg catttgatct gcaactctcg aaaaggcacg ctttaacatg tttgcagcta
     3481 caacaataac aacaccaatt gctatgtagt cagtaacaaa gacaacccaa ctttcgatat
     3541 tggtttttgg aacatcaggg gctgataatt gaaaaattcc caattgtgtc aaaa
//
LOCUS       DAOREF010000215         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16242_length_3591_cov_3.971719, whole genome shotgun sequence.
ACCESSION   DAOREF010000215 DAOREF010000000
VERSION     DAOREF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_3591_cov_3.971719"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKI33_05725"
     CDS             <1..215
                     /locus_tag="PKI33_05725"
                     /inference="COORDINATES: protein motif:HMM:NF021552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNM36537.1"
                     /translation="MCRSIKPLRNMDHPVTEQEIYEAALQYVRKVSGYRKPSKANEEA
                     FNKAIEEVAESTRILLAGLIVKQNIN"
     gene            224..1048
                     /locus_tag="PKI33_05730"
     CDS             224..1048
                     /locus_tag="PKI33_05730"
                     /inference="COORDINATES: protein motif:HMM:NF021057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HNM36538.1"
                     /translation="MEQLSHLAGIEYAGIAGASAECKHVIVRFIQNQDVISRARILTS
                     DNSHCFLLTVNGDLVAVKSGFASGYSGEGPRAFSYVLALLEFHNIEIEEYEVKPDVIE
                     RLDMTSLTIEDIEEIESSRPIRPKRLYEYINISREERRMLWQSFPSILPLSIIDPRIA
                     DLALDFWKDPDQKLTLGYRRLEDIVREKINSKEYGTGLFTEAFLSQNPKLKWENIENS
                     EQKGRGQLFVGAYMAYRNPRAHREIKDYSHQQLVEFLLLNQLYLLEQESYLVIEKG"
     gene            1119..1961
                     /locus_tag="PKI33_05735"
     CDS             1119..1961
                     /locus_tag="PKI33_05735"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNM36539.1"
                     /translation="MYRKLIPFLSILALTALACGISVPKPPTPQPEVTEDIKAEYPDA
                     DEVNLALRFGAGEMTLNPGADVLVDGTATYNYDELKPEVNTDGGDVEVRVGDGNLNIF
                     PNLNNLKNVWDLKLGDQPMNLSIESGAYDGTFELGGLSLTRLDIKDGAANVELAFSEP
                     NPVEMSTFTYSTGASDVKITGLANANFSFFDFSSGAGDYTLDFSGDLQRDASVKIETG
                     LSNFIIIVPEGVDAVVTVQGGLSNVNAGPGWERSGSDYIQKGEGPTITFVIEMGAGNL
                     TLAR"
     gene            2151..2564
                     /locus_tag="PKI33_05740"
     CDS             2151..2564
                     /locus_tag="PKI33_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNM36540.1"
                     /translation="MQITRQADYAVRAVLHLARNGETRTATSLIAEEQKIPPSFLAKI
                     ISQLSIAGLLHTSRGARGGVTLAREPKDITLLEVIEAIDGPIQLNECVGDTGTCSFDD
                     NCPLRPVWCEAQEQLVGRLKGTNFADMIAKGQTAA"
     gene            2655..3506
                     /locus_tag="PKI33_05745"
     CDS             2655..3506
                     /locus_tag="PKI33_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36541.1"
                     /translation="MKQHRGFLMAVLLMLTALACVVPGLQPASAIPPTPDTRLDRMVA
                     ETVAAALALTQQVPTATNTSTPTPTSTPTSTPLPVEPTQAEESVLDKNTDGTSSFIDL
                     SGKYQFTVPTQWLALRINSPEYDEAVLLPEAANPAIQNQLSVIQKQDPNVFRLFLLDI
                     NEEHIDSGFVTNVNLVWDPQMEISVATDSDLKGLAATLPTSLKDAEVTGVEIKTTTGG
                     LSYGVITATTPLMTQEGARLNVYQKLVFFDLPTGTLSVTLSTTEKWQSTVEPFFDEFV
                     TSFLILE"
BASE COUNT         1008 a          838 c          806 g          939 t
ORIGIN      
        1 ttatgtgccg aagcatcaaa ccccttagaa atatggacca ccccgttacc gagcaagaga
       61 tttacgaagc ggctcttcag tacgtgcgca aagtgagcgg atatcgcaag ccgtctaaag
      121 ccaatgaaga ggcttttaat aaggcgattg aagaagtggc agagtctaca agaatattgt
      181 tagcaggact aattgtcaaa caaaacatta attgagagta tcgatggaac aactatctca
      241 cttagctgga attgaatatg ctggtatcgc tggagcatct gccgaatgta agcatgttat
      301 tgtgcgcttt atccaaaatc aagatgtcat atctagagca agaatattga catcagataa
      361 cagtcactgt tttttgctta ctgtaaatgg tgacttagta gcagtaaagt caggctttgc
      421 gtcagggtat agtggcgaag gacctagagc cttttcttat gtccttgctc tgttagagtt
      481 tcataatatc gaaattgaag agtatgaggt gaaacctgat gtgatagaac gtttagatat
      541 gacatctttg acgatcgaag atattgaaga aatagaatcc tcaagaccaa ttcgtccgaa
      601 gcgcctttat gagtatataa atataagtag agaggaaaga cggatgttgt ggcaaagttt
      661 tccttcgata cttcctttgt ctattatcga tccacgcata gcagatttag ctttagattt
      721 ttggaaagac cctgatcaaa aattaacact agggtatcga cgtttagaag atattgtgag
      781 ggagaaaatt aatagtaaag aatatggcac tggattattt actgaagcat ttctctcaca
      841 aaaccccaaa ttgaagtggg aaaatataga gaatagcgag caaaaaggta gaggacaatt
      901 gtttgtaggt gcgtatatgg catatagaaa tcctagggct catcgtgaaa tcaaagatta
      961 ttcacatcaa caattagtag aatttctttt actaaatcaa ttgtatttac tggagcaaga
     1021 atcctattta gttattgaaa aaggttaata atttttgcaa ccaccttcca ctttccaccg
     1081 tataccttct tagtaataac ctaaggaggt ttttctttat gtaccgcaaa cttataccct
     1141 ttctctcaat tctcgcgctg acagcgttgg cgtgcggaat tagcgtaccc aaaccaccca
     1201 cacctcaacc cgaagtgacc gaagatatta aagctgaata tccagatgct gatgaggtca
     1261 acttggcatt gcgttttggc gcaggcgaaa tgactctcaa ccccggcgct gatgtgcttg
     1321 tagatggcac tgctacgtat aactacgacg aactcaaacc tgaagtcaac accgatggcg
     1381 gcgatgtgga agtccgcgtg ggagatggga atctcaacat cttcccgaat ctcaataacc
     1441 ttaagaacgt atgggatctg aaacttggcg atcagcccat gaacctgtct attgagtctg
     1501 gcgcttatga tggcaccttc gaattaggcg gactctccct cacgcgcttg gatatcaagg
     1561 acggcgctgc aaacgtagaa ctcgctttct ctgaaccaaa tcccgttgag atgagcacct
     1621 ttacctatag cactggcgct tcggatgtga agatcactgg gctggcaaat gccaacttta
     1681 gcttcttcga cttttcttcc ggcgcaggtg actacactct cgatttctct ggtgacttgc
     1741 agcgcgatgc ttcggtcaag atcgagaccg gcttaagcaa tttcatcatc attgtgcccg
     1801 aaggggtcga cgcagtggtc acagtgcagg gtgggctgtc gaatgtgaat gcaggtcccg
     1861 gctgggagag aagcggaagc gattacatcc aaaaaggaga aggtccaacc atcacctttg
     1921 tcatcgagat gggggcaggg aatctgacct tggcacgtta ggaaagaatt ttacaagttg
     1981 aaaaaaaacg gattacgtta caagtaatcc gtttttcgtt taaaaaccat ctttctaatt
     2041 aagcgcgaaa ggtcataatt catcagtgac ctctatcact tgccttgtaa cgccgttcac
     2101 aataaaatcg tgattgtggg taagttttac ctatattaag tgaggtcctt atgcaaataa
     2161 cccgacaagc cgattatgct gtacgcgctg tgctccatct tgctcgcaat ggcgaaaccc
     2221 gcactgccac cagcttaatc gctgaagaac aaaaaattcc accctccttc cttgcgaaga
     2281 tcatttctca actttcgatt gccgggttgt tgcacacctc ccgcggagcg cgcggcggtg
     2341 taacccttgc gcgtgaaccg aaagatatta ctctgcttga agtcatcgaa gctatcgacg
     2401 gtcccattca gctcaatgaa tgtgtaggcg ataccggaac ttgctccttt gacgataact
     2461 gtccgctgcg cccggtatgg tgcgaagcac aagaacagtt ggttggtcgc ttgaaaggca
     2521 ccaactttgc agatatgatc gccaaaggtc aaactgcggc gtaaggtcaa agaaatcaaa
     2581 gcctctcggc tataatagcc gggaggcttt tttttatata ccctatgggt aattccattt
     2641 gcaaggataa acctatgaaa caacaccgtg gatttttgat ggcagtcttg ctcatgttga
     2701 ctgctttggc atgtgtcgtg ccgggattac aacccgcctc tgcgattccg ccgacacctg
     2761 atacacgact tgaccgcatg gtcgctgaga ccgttgccgc cgctctcgcg ttaacgcaac
     2821 aagttcctac agccaccaac acttcaaccc cgaccccaac cagcacaccc acaagcacgc
     2881 ctctgcctgt tgagccaacc caagccgaag aaagtgtctt ggataagaac actgatggca
     2941 ccagctcttt cattgacttg agcggtaagt atcaatttac ggttcccaca cagtggcttg
     3001 cgttgcgcat caactcaccg gaatatgatg aagctgtgtt gttgcccgaa gcggcaaacc
     3061 ccgcaattca aaatcaactc tcggtcatcc aaaaacaaga cccgaatgta ttccgtcttt
     3121 tcttgcttga tatcaatgaa gaacatatcg acagcggctt tgtcaccaac gtcaatttgg
     3181 tatgggaccc gcaaatggaa atttcggtcg ccaccgattc agacctcaaa ggtttggcgg
     3241 ctactctgcc cacgtctctc aaagatgccg aagtgaccgg tgttgagatc aaaactacaa
     3301 ccggcggttt atcgtatggc gttatcactg ccaccacgcc tctcatgacc caagaaggcg
     3361 cccggttgaa tgtgtatcaa aaattggtat tttttgatct gcctaccgga accttatctg
     3421 tgactctttc caccactgaa aagtggcaaa gcacagttga accattcttt gatgaattcg
     3481 ttacctcttt cctcatattg gaataacgga gactgacatg tccacacccc gtgttatccg
     3541 cgcgccacgc ggcaccgaac tgacttgcaa gaactggctc agcgaagctg c
//
LOCUS       DAOREF010000216         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16255_length_3589_cov_4.573571, whole genome shotgun sequence.
ACCESSION   DAOREF010000216 DAOREF010000000
VERSION     DAOREF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16255_length_3589_cov_4.573571"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3589)
                     /locus_tag="PKI33_05750"
     CDS             complement(<1..>3589)
                     /locus_tag="PKI33_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM36542.1"
                     /translation="QSNGEVGGLIFFSEVITERKLADEKLRESERKYRELINGMNDTV
                     WVIDFDTRFLDVNDAAVKTLGYTREELLSMKVSDIDSGIEPEKIKQLIDRLKVEKRQV
                     FETSHVTKNGRKIPIEVSSSLVSYMGEMVVMSIARDITDRKQAEEEARLAEQRYRSLI
                     ENAPDGIVLVGIDGHFKYASPSVRRLFGYSIDETLQSMPDQFTHPEDLPGVLKELEHL
                     VADPTYIPTLQYRFLHKNGEWRWIESTFSNLVSVPSVEAIIINFRDIHERKLAEEALT
                     KSQALLTEAQRIGRIGHMEWNGSGQVLICSDELYDILEIPYEAQISKEIISNMMKPGE
                     AERIHELDMQAIQNHTDMDYEYCIQLRDGRERWLHQQGKFSYNQDGRPIRMLSIVQDI
                     TERKQTEEALRAEQARLAGIADTIPGAILTFQLCPDGTFNIPYASKAFEDLYGLARDD
                     VRKNIDSLSARVPHNQMEKLLQSVNESAKTLQPWRNEYAYHHPTKGMIWIEGFSMPMR
                     EADGTILWNGFASDVTERKTAEATLQESRLRLEMALKGANAGMWDWNVQTGETVFNER
                     WADIVGYSLQELEPISIQTWADLCHPDDLKLSDALLQKHFARETEFYECEVRMRHKSG
                     SWVWVVDRGKVMEWDANGKPLRMFGTHLNITERKLSEEEIRKRGEDLLLINTLNDAAN
                     RGEPLSNILNTLIQDARNMFDCQDLSVYLLSPDGKYAELQNLTLSGKRVERIEKLIGR
                     AIPRVQVPIREDGYFKQLLSNEQGTLIEDTASIRQWIEEFVDTPTLPDIVRPLIRKAI
                     PQIQKVIDINTIFAMPLFFSGNVIGLIELSSHTSFDKDDLKRIRTISRQMSAIILRIW
                     AVEKLRESEEKYRTLYKEMPIGVLLVSVSGEVLEANSATLQILGSPSIEATRQINLLT
                     FAPLIEAGLAADFKKTVDSEQINRVERHYLTKWGKSVHLYVTFVPVITPQQELVVQVL
                     IEDITARKDAENNLRASEEKYRSLIKSLNNSISIVDANGTFLYLNDMAGKELGGPPAE
                     LIGKTMHDLFPEPFSSMQLELVRNVIKIDHGYVYEAQSVVNNELRWFRVNFQPLHNEN
                     GEVNQVLINANDIHDLKVAQDELQKLNRNLEERVKERTAEVQDLYENAPTGYHSIDAD
                     GKFVRVNKTILDWLGFTRDEMMGSPISGFMTSATQKMFSEKFSELVQHGL"
BASE COUNT          925 a          882 c          693 g         1089 t
ORIGIN      
        1 caagccatgt tgcaccagct cagaaaattt ctcgctaaac atcttttgcg tggcactggt
       61 cataaaacca gaaataggcg agcccatcat ttcatctctg gtaaatccca accagtctaa
      121 aattgtttta ttaacccgca caaacttccc atctgcatca atagaatggt agcccgttgg
      181 cgcattttca taaaggtcct gcacctctgc ggttctttct tttacacgtt cttccaaatt
      241 acggttcagt ttttgtagtt cgtcctgcgc tactttaagg tcatgaatgt cgttggcatt
      301 aatcaagacc tgatttacct ctccattttc attatgcaga ggctgaaaat taacccggaa
      361 ccatcgcaat tcattgttaa ctacactttg cgcttcgtag acatatccgt gatcaatctt
      421 gataacattg cgcactaatt ccaattgcat ggaagaaaat ggttctggga ataaatcgtg
      481 cattgttttc ccaattaatt ccgctggagg tccacccaac tccttacctg ccatatcatt
      541 caaatacaaa aaggttccgt ttgcatctac gatagaaata gaattattta agctcttgat
      601 caaactgcga tatttttctt cgctggcgcg cagattattc tctgcatctt tcctagctgt
      661 aatatcttca atcaagactt gaacgaccag ttcctgttgt ggagtgatga ccggcacaaa
      721 ggtcacatac agatgaaccg actttcccca tttggttaag taatgtcttt ctactctatt
      781 aatctgttca gaatcaactg ttttcttgaa atctgcagcc agcccagcct cgatcagagg
      841 cgcaaaggtc aacaggttga tttgccgcgt agcctctatc gatggagaac ctaaaatctg
      901 caaggtcgcc gagttggctt ccaagacctc tccgctcaca cttaccaaca aaacaccaat
      961 cggcatctct ttatacaagg tgcgatattt ttcttcgctt tcacggagtt tttccactgc
     1021 ccagatccgc aaaatgatcg ccgacatttg gcggctgatg gtacgaatcc gcttcaggtc
     1081 gtctttatca aaggatgtgt ggctcgaaag ttcaatcaag ccaatgacat tccctgagaa
     1141 aaagagcggc atcgcgaaga tcgtattaat atcgatcact ttttgaattt gcggaattgc
     1201 ttttcttatc aatggacgca cgatgtctgg caaggtaggt gtatctacaa attcttcaat
     1261 ccactgtcgg atcgaagccg tgtcttcgat cagtgtgcct tgttcgttgg ataaaagctg
     1321 cttgaagtaa ccatcttcgc gaatggggac ttgcacacgg ggtatcgcgc gcccaatcaa
     1381 tttttcgatc cgctcaacgc gtttaccaga aagggttaag ttctgaagct cagcatactt
     1441 cccatctggg cttagtaaat ataccgacaa gtcctggcaa tcaaacatat tgcgtgcatc
     1501 ttgaataagc gtattcaaga tatttgataa aggctcaccc cggttggcag catcgttcaa
     1561 ggtgttgata agaagcaagt cttcaccgcg cttccgtatc tcttcttcag ataatttcct
     1621 ttcggtaata ttcaggtgcg taccaaacat ccgcaaaggc tttccattgg catcccattc
     1681 catcactttg ccgcggtcta ccacccaaac ccatgagcca cttttatgac gcatccgaac
     1741 ttcacattcg taaaactccg tctctctggc aaaatgtttt tgcagtaatg catcagagag
     1801 tttcaggtca tctggatggc ataaatctgc ccaggtctga atactgattg gttcaagttc
     1861 ttgcaatgaa taacctacga tatctgccca acgctcattg aaaacagtct ctcccgtttg
     1921 cacattccaa tcccacatgc cagcattggc acctttcaat gccatttcga gccgtaagcg
     1981 gctctcttgc agggtggctt cagccgtctt tctttctgta acatcagaag caaaaccgtt
     2041 ccacaaaatg gttccatccg cttctcgcat gggcatggaa aacccttcaa tccaaatcat
     2101 cccttttgta ggatggtgat acgcgtattc atttcgccaa ggttgtaatg ttttcgctga
     2161 ttcatttaca ctctgcaata gtttttccat ttggttgtgc ggaactcttg ccgaaagcga
     2221 atcgatattt ttacgaacat catcacgtgc aagcccatat aggtcttcaa aagctttact
     2281 ggcatatgga atattgaaag tgccatctgg gcaaagctgg aaggtcagaa tcgcaccagg
     2341 gatggtatcc gcaatccctg ccaaacgcgc ttgctctgct cgtaaggctt cttctgtttg
     2401 tttcctctcc gtaatatcct gtacgattga caacatacga ataggacgtc catcctgatt
     2461 gtatgaaaac tttccttgtt ggtgaagcca gcgctcacgt ccatctctta attggataca
     2521 gtattcataa tccatatccg tatgattctg aatggcttgc atatccagct cgtgaatgcg
     2581 ttctgcttcc cccggtttca tcatgtttga aatgatttct tttgatattt gtgcctcgta
     2641 cggtatctca aggatatcgt aaagctcatc tgaacatata agaacttgtc cgctgccgtt
     2701 ccattccata tgaccaatac gcccaatgcg ttgagcctcg gtcagcaatg cctgactttt
     2761 ggttaaagct tcttcagcta atttccgctc atgaatatca cggaagttaa taataatggc
     2821 ttcaacgctg ggcacagaaa ccaaattact gaatgtactt tcaatccaac gccattctcc
     2881 atttttatga aggaaacggt attgtagggt cggtatatat gttgggtctg cgaccaaatg
     2941 ctcaagttct ttcaacaccc cgggcaggtc ttcaggatga gtaaattgat ccggcatgct
     3001 ctgtaacgtc tcatcgatac tgtaaccaaa gagccttctc accgatgggc tggcatactt
     3061 aaagtgaccg tcaatgccta ctagcacaat tccgtctggg gcattttcaa tcagggagcg
     3121 ataacgttgt tcagcgaggc gagcttcttc ctctgcttgt ttacggtcag taatgtcacg
     3181 tgcaatactc attaccacca tttcccccat ataggaaacc aaactactac taacctcgat
     3241 gggtatcttt ctgccattct ttgtgacatg gctagtttca aatacctgtc ttttttctac
     3301 ctttaatcta tcaataagtt gcttgatttt ctccggttca attccagaat cgatatctga
     3361 gactttcatg gagagtaatt cttctcgtgt atagcccaat gtctttacag cagcatcatt
     3421 gacatccaga aaacgggtat caaaatcaat cacccaaacg gtgtcattca tgccgttgat
     3481 aagttcgcgg tattttctct cgctttcgcg taatttttca tctgctagtt ttcgttcggt
     3541 aattacttca gaaaagaaaa tgagcccgcc cacttcgcca ttcgattgg
//
LOCUS       DAOREF010000217         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16264_length_3588_cov_4.091990, whole genome shotgun sequence.
ACCESSION   DAOREF010000217 DAOREF010000000
VERSION     DAOREF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16264_length_3588_cov_4.091990"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..3372)
                     /locus_tag="PKI33_05755"
     CDS             complement(64..3372)
                     /locus_tag="PKI33_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36543.1"
                     /translation="MPKKPKITKRLDDLFKDVKPEENAKGRATKPKSQKEAPPPLPAD
                     PKPAQPAQAASTPVKTTRRQTGTLALPSKFIGRDTNAEAKSSYATTIQLGEEEWATLR
                     LVDNQSERSFSTDEQLLIRQVTDQLSLALENARLFQETQRRAQELEIVNKVVSAASGS
                     LDLQSTLQSVADNLVSLLDAQNVGIALINDEKTHLVLAAEAFSTLEISTGIGLLIPIE
                     GNLSTEEVLRTRKPLFIRDVDNNPLLVDSVREALQQRSTKNLLILPLNSEQEVIGTIG
                     VAFFDANRVIAQDELRILETIVAQIAAAVQKNRLFTQVEQSETNFRALFSAMEDVVLV
                     INREGRYMQIAPTNPSLLVRTPSDMLGRTFHELLPIDIADSFLSKVQEALVSDTKVQF
                     EYELAISGQLFWFLATLTKLDENSVFWVARDITERKKSEEAIQRRNQYLAVSAEIGKL
                     VTSILDLSTIFARTVNLITDRFNVYHAAIFIVEETGFNAVLKEGTGEAGAEMKQMEHS
                     LQLNDKSIVGSVASTGQLVVANNVLENPLYMAHPLLAETRSELAIPLKIGTRVIGVID
                     MQSRELNSFNEDEVSVLQTLADQVAVAIDNARSFELSQQAVMEMREADRLKSQFLANM
                     SHELRTPLNSIIGFSRVILKGIDGPVTELMQQDLTAIYNSGQHLLGLINDILDSAKIE
                     AGKMELAFDEVNIQDLTHSVLSTMSGLIKDKPIEMRQFIEQDTPTVRADAIRIRQVMI
                     NLLSNAAKFTEEGSITVNVAPYREDGKKLVKISVTDTGSGISIQDQEKLFQAFSQVDA
                     SPTRKSGGTGLGLSICKQLINMHGGQIWVESKESEGSTFHFTLPLFNKENELAEEGNK
                     VILTIDDDPQVIGLYERYLQPQGYHVHSLTDPSRAVEVAKQVKPFAITLDIMMPGTDG
                     WTVLDTLKSDSATRNIPVIICSIVEDIEKGFNLGASDYLVKPILEEDLVNSLDRLNAD
                     GSIREVLVIDDNPNDLRLIGKMLNDDGRYKPILAEGGRNGWTIISSGTPPHAIILDLF
                     MPEMDGIQILEQLQSDKRLRDIPTIVISGMDVTPEQKNKLKEFGQRLLTKGSFNESEL
                     LTSIQRSLDRLSKK"
     gene            complement(3338..>3588)
                     /locus_tag="PKI33_05760"
     CDS             complement(3338..>3588)
                     /locus_tag="PKI33_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36544.1"
                     /translation="ILVTMLDRRNRTHRNIHEQLRATFGSGVFNTVIELDTKLRESPI
                     AGQPITHYKSAARGSTQYRVLAQELMEYAKETENHQAS"
BASE COUNT          934 a          795 c          828 g         1031 t
ORIGIN      
        1 gcgttatgcc cacaatctag gcacttaata ataaatgaca cttaattctc ctgtagactt
       61 cgtctatttc ttagacaagc ggtcaagcga gcgttgaatc gaagttagta attcactttc
      121 gttaaaggag cccttggtga gtaaccgttg accaaattct ttgagcttat tcttctgttc
      181 tggcgtcaca tccatgccgc tgatcacaat ggttggaata tcacgcaggc gtttatccga
      241 ttgtaactgc tcaagaatct gaataccatc catttctggc atgaacaaat ccaagatgat
      301 cgcgtgaggc ggtgtaccag aggagataat cgtccaacca ttgcgtccgc cttcagcaag
      361 gatgggttta tatcgtccat cgtcattcag cattttgcca atcaaacgca ggtcattggg
      421 gttgtcatca atcacaagca cttcacgaat ggagccatcc gcgttcaagc gatctaacga
      481 attgaccaag tcttcttcaa gaataggctt taccaagtaa tcggaagcgc caaggttgaa
      541 acctttttca atatcttcaa cgatactaca tatgataacc ggaatattcc gggttgcaga
      601 gtcagatttg agcgtatcca aaacagtcca gccgtcggtg ccgggcatca taatatcaag
      661 agtaattgca aaaggcttta cttgtttggc aacttctact gctcgcgaag gatctgttaa
      721 tgagtggacg tgatagcctt gcggttggag gtaacgttca tacaaaccaa tcacttgtgg
      781 gtcatcatca atagttaaga taaccttatt gccttcctct gccaattcgt tttctttatt
      841 gaacagcggc aaagtgaagt ggaacgtact tccttcactt tctttacttt ctacccagat
      901 ctgcccacca tgcatattga ttaattgttt acagatggaa agacctaagc ctgtgccacc
      961 gcttttgcgg gtaggagaag catctacttg agaaaaggct tggaagagtt tttcttggtc
     1021 ttggattgaa ataccagagc ctgtatcggt cacactgatt ttgactagtt tcttcccatc
     1081 ttcacgataa ggagcaacgt taaccgtgat ggagccttcc tctgtgaatt tagctgcatt
     1141 agaaagcagg ttgatcatga cttgacggat acgaatcgca tcggcgcgga cggttggcgt
     1201 atcttgctcg ataaactgcc gcatctcgat tggtttgtct ttgatcaaac cgctcatcgt
     1261 agacaataca ctgtgagtca ggtcttgaat attaacttcg tcgaacgcca attccatttt
     1321 gccggcttcg atcttggcag agtcgaggat atcgttgatg agaccaagca agtgttgacc
     1381 agagttataa attgcggtta ggtcttgctg cattaactca gtgacaggtc cgtcaatacc
     1441 ttttagaata acgcgtgaaa agccaatgat ggaattcaaa ggcgtacgca actcatgact
     1501 catatttgcg aggaactggc tttttaaccg gtctgcctcg cgcatttcca taacggcttg
     1561 ttgtgagagt tcaaaggagc gggcattgtc gatagcaaca gccacttggt ctgcaagggt
     1621 ttgaagaacc gaaacttcat cctcattgaa tgagtttagt tctcgtgatt gcatgtcaat
     1681 cacaccgatt acacgtgtac cgatcttgag cggaatggca agctctgaac gtgtctctgc
     1741 caataatgga tgtgccatgt aaagcggatt ttcaagaaca ttatttgcaa ccaccaattg
     1801 cccagtagat gcaaccgagc ctacaatgga tttgtcgttc aattgcaaag aatgttccat
     1861 ttgtttcatt tcagcgccag cttctccggt tccttctttc agaaccgcat taaagccagt
     1921 ttcttctaca ataaaaattg cggcatggta cacattgaaa cgatctgtga tgaggtttac
     1981 ggtacgggca aagatggtgc tcaggtcgag gatagaagtg accaatttac cgatttcagc
     2041 tgataccgcc aaatactgat ttctgcgttg aatcgcttct tcagattttt tgcgttctgt
     2101 aatatctctc gccacccaaa agacgctatt ctcatctaac tttgtcaggg tcgcgaggaa
     2161 ccaaaaaagt tgtccagata tagcaagttc atactcaaac tgtacttttg tatcacttac
     2221 taaagcttct tgaacctttg aaagaaatga gtcggcaata tctatcggca aaagctcatg
     2281 gaaggttctc cccagcatgt ctgaaggggt gcgaaccaag agagatgggt tggtgggggc
     2341 aatttgcata taacgccctt cacggttgat aaccaacacc acatcttcca ttgccgagaa
     2401 tagcgcgcgg aagttcgttt cagattgctc tacttgagta aacaacctgt tcttttgtac
     2461 agccgcagcg atctgggcaa caatggtctc aagaatgcga agttcatctt gagcaataac
     2521 acggttggca tcgaaaaatg caacgccaat ggttcctatc acttcttgct cagaattcaa
     2581 gggcaagatc agtaaatttt tggtgctccg ttgttgaagt gcttctcgaa cagagtccac
     2641 taggagcgga ttgttatcta cgtccctgat aaataatggt ttacgagtgc gtaagacttc
     2701 ttctgtgctg agattgccct caattggaat taacagtcca attcccgttg aaatttctag
     2761 ggttgagaat gcttctgcag caaggacaag atgtgtcttt tcatcgttga ttagcgcaat
     2821 accaacattt tgtgcatcca acaagcttac aaggttatcg gcaacacttt gcaaggtact
     2881 ttgcaggtca agcgaaccag atgccgcaga cacgaccttg ttaacgattt caagctcttg
     2941 tgcgcggcgc tgtgtctctt ggaacagacg tgcattttct aacgccagcg ataactggtc
     3001 ggtcacttgc cgaataagca attgctcatc agttgaaaat gagcgttcag actgattatc
     3061 tacaaggcga agagttgccc attcttcttc tccaagttga atcgttgtag catacgaaga
     3121 cttcgcttcg gcatttgtat cacgcccaat gaactttgaa ggcaaagcaa gcgtacccgt
     3181 ttggcgacgg gttgtcttta ccggtgtaga tgcagcttgg gcgggttgcg caggcttcgg
     3241 gtccgcagga agaggcggtg gcgcttcttt ctgtgacttg ggcttggttg cccgtccctt
     3301 ggcattctct tcaggtttta catccttgaa gagatcatca agacgcttgg tgattttcgg
     3361 tttctttggc atattccatt aattcctggg cgaggacacg atattgggta gaaccacggg
     3421 cagcagactt gtaatgtgta ataggctgtc cagctattgg gctttctcga agtttagtat
     3481 ctaactcgat aacggtgttg aagaccccag aaccaaatgt ggcacggagt tgttcatgga
     3541 tgttgcgatg cgtgcggtta cgtcggtcta gcatggtaac aaggatac
//
LOCUS       DAOREF010000218         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16289_length_3585_cov_3.422096, whole genome shotgun sequence.
ACCESSION   DAOREF010000218 DAOREF010000000
VERSION     DAOREF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16289_length_3585_cov_3.422096"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PKI33_05765"
     CDS             complement(<1..1131)
                     /locus_tag="PKI33_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36545.1"
                     /translation="MQFRLSPITRQTLLIAVITIIAYGLLLPLTGFYWDDWPFAWIAK
                     FLGPAEFNPAFMPFRPFLGPIFYFTTTVIPLNPLAWQIFALVIRFLIGVTAWWTFTQI
                     FPQRKTIAFFAALLMLVFPGYSQHWVALTHINQELIPFLFYLLSFGYTFKALHTKKKT
                     DTVIALLLQLCGIFPTEYFFGVEGIRFFLLFFVFQGTILERFTKTLKVWFPYLLIWFL
                     NAAWLFYYYKFGPYESYEVAAAQSPNLFYFLTQALDALWKAGFYIWAQVLVLTASTLH
                     APASLLTLGLVAISFILLVWVLIRSTQAEATDKSIAISLLTIGFFGILLGRLPSLAAN
                     LPLTLQSSYDRFMISIMLGSVLFVLGLLELFVKDHRTRVIVLS"
     gene            complement(1209..>1827)
                     /locus_tag="PKI33_05770"
     CDS             complement(1209..>1827)
                     /locus_tag="PKI33_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36546.1"
                     /translation="VIVLLNIYGISSNSKWNKHTEIKKGSPMPTTSLTHRLAKREDLI
                     ALKALMDAAISENQKAFLDESQIASSRTIMGLDTQLIDDGTYFIVEIDGQLAGCGGWS
                     RRATMYGSDQTPGRSAALLDPTKDPARIRAMYTHPNHVRKGIGKLIITLCEEAAKAEG
                     FTKMELVATLSGEPLYRTCGFEAYENIVDDRGGAGVPLLRMRKSL"
     assembly_gap    1828..1927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1929..2540)
                     /locus_tag="PKI33_05775"
     CDS             complement(1929..2540)
                     /locus_tag="PKI33_05775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNM36547.1"
                     /translation="MKEEIHITFLRHGRSRADDENVHEGRYDSPLTDVGRSQVQARVQ
                     EFLFQQFHFDAVISSPLQRAHETATIIRQALSLPIETDADWMEMDNGPLAGMPWDEAE
                     KQYPKPAFRNPYEPFCGSGESDWELYTRGAKAVEKIIRRGPGSYLVVAHGGILNSALR
                     TIIGTQPSINQQGITFGFGDTGYARFSYRPAQHLWYLLEFKME"
     gene            complement(2577..2879)
                     /locus_tag="PKI33_05780"
     CDS             complement(2577..2879)
                     /locus_tag="PKI33_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNM36548.1"
                     /translation="MTTDPTFRMTVQDIFFIKGRGTVVTGRVESGIITVGDEIRIQGK
                     SANKTAIVTGVEVLRKVTTQAKAGDNIGLLFKDITKEDVQVGDILTGSDLDFTWKP"
     gene            complement(2890..>3585)
                     /locus_tag="PKI33_05785"
     CDS             complement(2890..>3585)
                     /locus_tag="PKI33_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNM36549.1"
                     /translation="VHQLLLHLEAKGFVGAPRFLGIDEQQREILSFIEGETTFSEALW
                     QADAPLVAAAQLLKNYHDATLDFPQKDGLVWGITSPDPARHEVICHNDFAPYNFIFQA
                     QSPYAVIDFDLVGPGPRLRDVAYAAYWLTPLSFHRADLLPFTEADVKNDSRRLHLFCE
                     TYGIRASEALFDMVYEVLCFMGDKTKMEEILGDEIAAKLEREGHVKHWQAEAQAFLEN
                     RNRFAKNLSQPDQ"
BASE COUNT          936 a          728 c          942 g          879 t
ORIGIN      
        1 agaaaggaca atgacacgtg tgcgatggtc tttgacgaat aactctagca aacctaacac
       61 gaataagacc gaaccgagca tgatagagat cataaaacgg tcgtaggagg attgcagagt
      121 taggggaaga ttggctgcaa gagagggaag gcgccctaag agaataccga agaagccaat
      181 tgttaataag gagatggcaa tggatttatc tgtcgcttcg gcttgggtgg agcggattaa
      241 gacccagacg agcaaaatga atgagatggc gaccaagccc aaggtcagaa gagaggcggg
      301 cgcgtgtagg gtggaagcgg ttaagacgag gacttgagcc caaatgtaga acccggcttt
      361 ccaaagagca tccagcgctt gtgttagaaa gtaaaacaga ttaggcgatt gcgcggcggc
      421 aacttcgtag gattcatagg gaccgaattt atagtaatag aaaagccagg cggcattgag
      481 aaaccagata agtaaatagg ggaaccagac tttgagtgtt ttcgtgaagc gttcgaggat
      541 ggtgccttga aaaacgaaga agagcaaaaa gaagcgaata ccttcgacgc cgaagaaata
      601 ttcggtgggg aagatgccac agagctgtag aagtagtgcg atgaccgtat ctgtcttttt
      661 cttggtatgc agggctttga aggtgtagcc gaaagaaagt agataaaaca gaaaggggat
      721 gagctcttgg ttgatgtgtg tgagcgccac ccaatgttgg ctataaccgg ggaaaaccag
      781 catgagcaaa gccgcgaaga atgcaatcgt ttttcgttgt gggaagattt gagtgaaggt
      841 ccaccaggcg gtgacaccga tgagaaagcg gatgaccaac gcaaagattt gccatgccaa
      901 cgggttaagc ggaatgacag tagtagtgaa ataaaagata ggacctaaaa acgggcgaaa
      961 gggcatgaac gccgggttga actcggcagg accaagaaat ttggcgatcc atgcgaaggg
     1021 ccagtcgtcc cagtagaagc cagtgagcgg taagagcaac ccataggcga tgatggtgat
     1081 aaccgcaatg agcaaggttt gacgtgttat gggggagagg cgaaattgca tcctttaatt
     1141 ataagggatg gcgaggttga gtcttaattc ttgccctgta agatgtagag ataaggaata
     1201 caaaattttt acagcgattt tcgcattcgc aagagcggaa cgcccgcacc accgcgatcg
     1261 tcgacgatat tttcgtaagc ttcgaatccg caggtgcgat acagtggttc gccagataag
     1321 gtggcgacca attccatttt tgtgaagcct tctgctttgg cggcttcttc acatagggtg
     1381 atgatgagtt tcccgattcc tttgcggacg tggttaggat gcgtgtacat ggcgcggata
     1441 cgagccgggt ctttggtggg gtcgagcagg gcggcactgc gccctggggt ttggtcactg
     1501 ccatacatgg tggcacgtcg actccacccg ccgcagcctg ccagttgacc atcgatttcg
     1561 acgatgaagt aggttccgtc atcgatgagt tgcgtatcca accccataat ggtacggctc
     1621 gaagcgatct gactttcatc gagaaaggct ttttgatttt ctgagatagc ggcgtccatt
     1681 aaggctttaa gggctatcag gtcttctcgt ttggcaagtc tgtgggttag agaggttgtg
     1741 ggcatggggc ttcctttttt aatttcagtg tgtttattcc attttgaatt cgaggagata
     1801 ccatagatgt tgagcaggac gataactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnttt attccatttt gaattcgagg agataccata gatgttgagc aggacgataa
     1981 ctaaagcggg cgtacccagt atcaccaaaa ccaaatgtga tgccctgttg gttgatagat
     2041 ggttgtgtgc cgataattgt ccgtaaagca gaattgagaa tgccaccatg ggcgacgact
     2101 aaataacttc cgggtccgcg ccgaatgatt ttttctacgg ctttggctcc gcgagtgtaa
     2161 agctcccagt cgctttctcc tgaaccgcaa aacggttcat atggatttcg aaatgcaggt
     2221 ttgggatatt gtttttcggc ttcgtcccaa ggcatgccag ctaagggacc attatccatt
     2281 tccatccaat ctgcatccgt ttcaatgggg agactcaacg cttgcctaat gatcgtggcg
     2341 gtttcatgtg ctcgttgcaa cgggcttgaa ataaccgcat caaaatgaaa ttgttggaag
     2401 agaaactctt gcacgcgggc ttggacttgg gaacgcccta catcagtgag aggtgagtcg
     2461 taccggcctt cgtgaacgtt ttcgtcatcg gcgcgagacc gcccgtggcg caggaaggtg
     2521 atatggattt cttctttcat gagtactccc aaaataacaa gatcggataa ggtcgtttat
     2581 ggcttccagg tgaaatccaa atcagaccct gttaggatat ccccaacttg aacatcttct
     2641 ttcgtgatgt ctttgaacag tagtccgata ttgtcaccag cttttgcctg tgttgtgact
     2701 ttgcgaagga cttctacccc cgttactata gccgttttgt tggcgctttt cccttggatt
     2761 cgaatttcat ctccgaccgt gattattccg ctttcaacgc gaccagttac aacggttccg
     2821 cgtcctttta taaagaagat atcttgtacg gtcatgcgaa aagtagggtc tgtggtcatg
     2881 gggaatcctt tattgatcgg gctgagacag atttttagcg aagcgatttc gattttctag
     2941 aaaagcttgg gcttctgctt gccagtgttt tacatgtcct tctcgttcaa gttttgcggc
     3001 aatttcatcc cctaggattt cttccatttt cgttttgtca cccataaaac ataaaacctc
     3061 atagaccata tcaaaaagag cctcgctggc acggatgcca taggtctcgc aaaacaggtg
     3121 aagacggcga ctgtcatttt ttacgtccgc ttctgtaaaa ggaagcaggt cagcacgatg
     3181 aaatgataag ggtgtaagcc aataagcggc atatgccaca tctcgcaatc gaggacccgg
     3241 gcctaccaga tcaaaatcaa tcactgcata aggcgattgg gcttggaaga tgaaattata
     3301 aggggcaaag tcgttatgac agatgacttc atgccgagca gggtcaggag atgtgatgcc
     3361 ccacaccaga ccatcttttt ggggaaaatc caaggtggcg tcatgataat tcttcaagag
     3421 ttgagcggca gcaaccaggg gagcatccgc ttgccaaagt gcttctgaga acgtagtttc
     3481 cccttctata aaggataata tttcgcgttg ttgctcatca atgcctaaaa atcgtggagc
     3541 ccccacaaag cctttggctt ccaaatgtaa aagcagttgg tgtac
//
LOCUS       DAOREF010000219         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16427_length_3566_cov_4.315010, whole genome shotgun sequence.
ACCESSION   DAOREF010000219 DAOREF010000000
VERSION     DAOREF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16427_length_3566_cov_4.315010"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1033)
                     /locus_tag="PKI33_05790"
     CDS             complement(173..1033)
                     /locus_tag="PKI33_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNM36550.1"
                     /translation="MKIVTDCAADMSNEELKQLGIVQAPLFIQFPEGEVNSADISADE
                     FYNRLEAMRPQIPTTAQPSSGIFAELYRKLAQIDKNILSVHISSGLSGTINSARDGGE
                     QVKGEANVNFWDTLTLSGGERFQVMAAALADKAGWAMDKVQERLTKIREKTEVIYTLD
                     TLEYLARGGRIGRVQAMAGALLNLKPVIRVDTDGKYSTVAKGRSIGKSMSIIADHLVE
                     KYGNAPVWVTVLHGRFAEKADQLAEEFKQKLNVAKMEVQRISPVLGVHTGPGIVGAAV
                     VPMELMGDLV"
     gene            complement(1030..1662)
                     /locus_tag="PKI33_05795"
     CDS             complement(1030..1662)
                     /locus_tag="PKI33_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNM36551.1"
                     /translation="MQQRSEETRSKIIFSAVKLFSAKGYNTASVDDICADAGISKGAF
                     YHHFKTKQDLFLALLDGWLETIDNAIEASKDKTVPETFMQITEAFPYIFATANEGLPM
                     FLEFWLQASRDEKIWQASVKPYRRYHKYFTDLIKKGVDEGSFVEVDPDLASRMIISTA
                     MGLLLQSLMDPKGAKWEKVARDSTTMLVASMLKNKNTIQTTNQSNQKRKP"
     gene            complement(1822..2859)
                     /locus_tag="PKI33_05800"
     CDS             complement(1822..2859)
                     /locus_tag="PKI33_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNM36552.1"
                     /translation="MNLLEVLQFALSKVWETFSGLWPLLLLSVLISVILKLYVDQDRV
                     AAFLKRNQSNSIAMSTALAIGTPFCSCGTTAVVLGMMASTIPWAPIVAFMVASPLTSP
                     QELFYSAGLFGWPFAITFFVASIILGLTGGAIAAFAESKGWLANQGRFASDKAPRADL
                     KPAPVSQTVFQPQALLQGQAQFATQTAGISLSLPVIASEPIPTKKVVTFKQFADETFT
                     TSWKLLAMFLGFAFLGYFLNGLIPQEWIKTAFGSGNAYSVPLAAILGIPFYLNTEASM
                     PLVKAFMDSGMSAGAALAFLITGAGTSIGALAGAMTIARWRVIGIVLGTLVTGAIVLG
                     MLYDLLAPVFL"
     gene            complement(3168..3479)
                     /locus_tag="PKI33_05805"
     CDS             complement(3168..3479)
                     /locus_tag="PKI33_05805"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNM36553.1"
                     /translation="MKFDPVLFAKAIADETRQKIMSECCCCELSVSEIVEKIGFSQPT
                     ISHHLAILRDANLVNVREEGKQTFYTLNQEHIAVCCGQIMVKFAPESVTTENLLKTLK
                     A"
BASE COUNT          898 a          791 c          917 g          960 t
ORIGIN      
        1 tttggcaaac caaaaccttg gcgagatcga ccctgagaaa tgggtggtct tcatgttcta
       61 ctcccatccg ccattaggcg agcggattgc gaaagctaga aagtttacgg cttaaaagta
      121 taggtcacgc ttaaagcgtg acctatttat ttcttgtctt ccaatacttt tcttacacca
      181 gatctcccat caattccatc ggcacaactg ccgcaccgac aatgccggga cctgtatgca
      241 cgcccaatac aggcgagatg cgctgtactt ccattttggc gacattcaat ttttgtttga
      301 attcttcggc gagttgatct gctttctcag cgaagcgacc gtgcaaaacc gtaacccaaa
      361 ctggagcatt tccatatttc tcaacgagat ggtctgcgat aatggacatg gatttgccaa
      421 tggaacgtcc ctttgcaacg gtgctgtatt ttccgtctgt atccacacgg atgacaggct
      481 tgagattgag taaggctcca gccatggctt gcacacgtcc gatgcgacca ccacgagcga
      541 gatattccaa tgtatcgaga gtgtagatca cttcggtctt ctcgcggatt ttggtcaggc
      601 gctcctgaac tttgtccatt gcccaacccg ctttgtctgc caatgccgct gccatcactt
      661 gaaagcgctc tccaccagag agggtgagcg tatcccaaaa gttgacgttt gcttcgcctt
      721 tcacttgttc gccaccatct cgtgccgagt tgatcgtgcc gctcagtcca gaagagatat
      781 gcaccgatag aatattttta tctatttgtg ccagtttgcg gtacaactcc gcgaagatgc
      841 cgctggatgg ctgtgctgta gtcggaattt gtgggcgcat tgcctccaaa cgattgtaga
      901 actcatcggc agaaatatct gctgagttta cttcgccttc agggaattgg ataaacaggg
      961 gggcttgtac aattcctaac tgtttcaatt cctcattgga catatctgct gcgcagtctg
     1021 ttacgatttt catggtttcc tcttttggtt agattgattc gttgtttgaa tagtattttt
     1081 gtttttcaac atgctagcga ccagcatggt ggtgctgtca cgtgcgacct tttcccattt
     1141 tgcacctttg gggtccatga ggctttgtag caacaatccc attgcggttg aaatgatcat
     1201 gcgtgaggct aagtcagggt ccacttcgac gaatgagcct tcgtccacgc cttttttgat
     1261 gaggtcagtg aagtatttgt gatagcggcg ataaggcttc acgcttgctt gccatatctt
     1321 ttcatcgcgg ctggcttgta accagaactc aaggaacatg ggcaagcctt cgtttgccgt
     1381 tgcgaagata taggggaatg cttctgtgat ctgcatgaag gtctcaggga cagttttgtc
     1441 cttcgaggct tcgatggcgt tatcgatggt ttccagccag ccatcaagca gggcaaggaa
     1501 aaggtcttgc ttggtcttga agtgatgata gaacgcgcct ttgctaatgc cagcatccgc
     1561 gcagatatcg tctacactgg cggtgttgta gcctttggct gaaaacagtt tgacggctga
     1621 gaaaatgatc ttcgaacggg tttcttcact gcgttgttgc atcttgtcct tgagacgagt
     1681 cgggtgctgg aaaaggaata aaaccgactg gtcggtttgt ttttacctct gaaactttgc
     1741 actgtcaaga tgagggaact catgtttaaa agaaaagcgg acagaacctt tgggctctgt
     1801 ccgctatgtt gagttgaacg attataagaa tactggtgcc agtaaatcgt acaacatgcc
     1861 cagaacaata gcgcctgtta ccaaggttcc aaggacgatg ccgatcacac gccaacgggc
     1921 gatggtcatt gcacctgcca aggcgccgat agacgtcccc gcgccggtga tgaggaaggc
     1981 gagagccgcg cctgcactca tgccagagtc catgaaggct ttgacgagtg gcatagaggc
     2041 ttcggtgttg aggtagaagg gaatgccaag gatggcagcc aaaggaacgc tatatgcgtt
     2101 gccagaacca aacgctgtct taatccattc ttgggggatc aagccgttga ggaaataccc
     2161 aaggaaggcg aagccaagga acatagcaag cagtttccat gaggtggtga aggtttcgtc
     2221 tgcaaactgt ttgaaggtta cgaccttttt ggttggaatg ggttcggatg cgatcacagg
     2281 caatgacaat gaaataccag ctgtttgtgt cgcaaattgt gcctgtcctt gtaagagggc
     2341 ttggggctgg aaaacggttt gagaaactgg agccggcttg aggtctgctc gaggcgcttt
     2401 atctgatgcg aaacgtccct ggttggcgag ccatcctttt gattcggcga aggctgcaat
     2461 ggcgccgcca gtcaaaccca agatgatgga tgcaacgaag aatgtgatag cgaagggcca
     2521 gccaaacaag ccggcactat aaaaaagttc ttgcggagag gtgagagggg aggcgaccat
     2581 gaatgcaaca atgggcgccc atggaatggt ggatgccatc atgccaagca cgacggcggt
     2641 tgtaccgcat gagcagaaag gcgtgccaat tgctaacgct gtagacatgg caatgctgtt
     2701 actttggttg cgtttgagaa atgcagcgac acggtcttgg tcaacatata gtttgagaat
     2761 gacgctgatg agaacgctca agaggagcag gggccataag cctgaaaaag tttcccaaac
     2821 tttactgagt gcgaattgaa ggacttcaag aagattcatt gatcatctcc aaaataaaga
     2881 tgtgtgttta gaaggatatt catggtttta tctttcatat agaagtttat ctataagtaa
     2941 aagcaaaaaa atttaccaat aatgtctctc tgcctgccaa atggcaaaca gagatcgagc
     3001 ttagcagcaa ccgctggggc agttgccgcc ttcacaacat tcgctttcgc aacaatcgtt
     3061 ggtgacgaca ggtaattcgt tcatgggata ctcctttcgt atagaagttt gtctatggaa
     3121 ctgattgcaa aaaacgggat catgtcacgg ccgtggacat atcccggtta tgccttgagg
     3181 gttttcaaca ggttttcagt agtaacggat tcgggcgcaa acttgaccat gatctgtccg
     3241 caacatactg caatatgttc ctggttgagg gtatagaaag tttgtttgcc ttcttcgcgc
     3301 acattgacca aattggcatc gcgcaatatg gcgaggtgat gcgaaatggt gggctgtgaa
     3361 aagccaattt tctcgacgat ctcgctgacc gagagttcgc aacaacaaca ctcgctcatg
     3421 attttctggc gggtttcgtc tgcgatggct ttggcaaaca ggacagggtc aaatttcatg
     3481 cggttattat atagaagttt gtctatttgt caagaaaaaa atgacttctt tataaaacct
     3541 atttagcgca aatatttaat aatttc
//
LOCUS       DAOREF010000220         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16460_length_3562_cov_3.074708, whole genome shotgun sequence.
ACCESSION   DAOREF010000220 DAOREF010000000
VERSION     DAOREF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16460_length_3562_cov_3.074708"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKI33_05810"
     CDS             <1..425
                     /locus_tag="PKI33_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36554.1"
                     /translation="DLRLIYTPWAIIIAQTVIATPMVAGLTTAALEALDPRLKQQLLG
                     LGASQLQMVWHLWREARLPLLAALMAGFGSVISEVGASMMVGGNIRGQTRVLTTAIVL
                     ETSKGEFEQALALSALLLVITYLINLGLTWIQQKGKKR"
     gene            450..1169
                     /locus_tag="PKI33_05815"
     CDS             450..1169
                     /locus_tag="PKI33_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36555.1"
                     /translation="MANIIEISNLIVKRNGRETLRIDSLHIPKGETLAVVGPNGSGKS
                     TFLLTLAHLLKAASGDILLHGKHLKEWNELEYRRQIAFVFQDPLLMDMSVRENIRLGL
                     KFRKMDKKETDARVDKWATAMGVEKLLERRANQLSGGEAQRVSLARAFVLDPELLLMD
                     EPFSAVDPQTREKLLEDLSRVLAENQRTTIFVTHNLKEAAQFGDRVAVMIGGEMKQVG
                     APAQIKESPVDASVKDFLKGL"
     gene            1234..2502
                     /locus_tag="PKI33_05820"
     CDS             1234..2502
                     /locus_tag="PKI33_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNM36556.1"
                     /translation="MPEFLQLLPPEEARKLLFSNLPHAKIKQENIETASSLGRVIAED
                     ISAPHSLPEFLRSTVDGYAVRARDTFGASDSMPAYLSLVDEIPMGSAPSQNIGSGQCG
                     LIHTGGMLPPGADAVVMLENTQVALGGNRPTPGSEIEIFRAVADGENVIQIGEDVKSG
                     SVILKGGTRIRPAEVGGLMALGITHLSVAAKPKVGLISSGDEIIDPFKQTQPGQVRDI
                     NAYTLASLVTKTGGEPILYGIVPDVRADLESTTRRALNECDVVVITAGSSASTRDMTA
                     DVIASLGAPGVLVHGINTRPGKPTILGVCNGKAVIGLPGNPVSALVNGYLFVVPVIER
                     LLGETPRPRPIIAAKLTVNLPSQAGREDWIPVKLIAQSGNAGSQTFQAEPIFGKSNLI
                     FTLTAADGLLRIPPDVTGYGAGEMAEVVLF"
     gene            2538..>3562
                     /locus_tag="PKI33_05825"
     CDS             2538..>3562
                     /locus_tag="PKI33_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNM36557.1"
                     /translation="MSIYLHDIPLSTAQNHFRTSLEEVGLWRVLGSETIPLDENALGR
                     VLSEPIWASISSPHYHASAMDGFAVVAENTSGAGPSTPLTFPIEAESAHYVDTGDPLP
                     EWANAVIPIENVESLDENNTITSNIQRPASIRIRAAVSPWSHIRPLGEDIVATQLVLP
                     SGHTLRPADLGAIAASGHQSIQVARKPKVAILPTGTELVPIGSKLKSGDILEYNSLVL
                     AAQIKTMGGDPTRYPITKDDFDLICNNVSEAAQDHDLILLNAGSSAGMEDFSSKVVEK
                     LGQLLVHGVAVRPGHPVILGIIDRDGKKVPIVGVPGYPVSAALTTDIFVEPLIAKWLG
                     RRPLELPT"
BASE COUNT          854 a          898 c          950 g          860 t
ORIGIN      
        1 gtgatctgcg tctcatctac acgccgtggg caatcatcat cgcgcagacc gtgattgcca
       61 ccccgatggt ggcaggcttg accacagccg cactcgaagc gctcgatcca agattgaagc
      121 agcaactttt gggactcggc gcctcacaac tccagatggt ttggcacctg tggcgggagg
      181 ctcgtctgcc gctgttggcg gcgttgatgg caggcttcgg ctcggtcatc tcggaggtgg
      241 gcgcatccat gatggtgggc ggaaacatcc gcgggcagac gcgagtgcta accacggcga
      301 ttgtgttaga aacatccaaa ggcgaatttg aacaagcctt ggcgctctcg gcattgttac
      361 tggtgattac ttacctcatc aacctggggt tgacatggat tcaacagaaa ggcaaaaaac
      421 gatagtccag tctttcgttg aaaaacacca tggcaaatat cattgaaatt agcaatttga
      481 tcgttaagcg caacggacgc gagacattac gaatcgattc gcttcatatt cccaaaggcg
      541 aaacattggc tgtagtaggt ccgaatggtt cagggaaaag tacgtttcta cttacgctgg
      601 cgcatttgct taaggcagca agtggagaca ttcttttgca tggaaaacat ctcaaagaat
      661 ggaacgaact cgaatatcga cggcagattg cctttgtctt tcaagacccg ctactgatgg
      721 atatgtctgt acgggaaaac attcggctgg ggttgaagtt ccgcaagatg gataaaaaag
      781 aaaccgatgc gcgggttgat aagtgggcaa ctgccatggg cgtggaaaaa cttctggagc
      841 gcagagccaa ccaactctct gggggtgagg cgcagcgagt cagcttggca cgggcgtttg
      901 tccttgatcc cgaattgctt ttgatggacg aaccgttttc agcagtggac ccgcagaccc
      961 gtgaaaaatt acttgaagac ctttcgcgtg tgttggcaga gaatcagcgc accactattt
     1021 ttgtgactca caatttgaaa gaagcggctc agtttggcga ccgtgtggca gtgatgattg
     1081 gcggcgagat gaagcaagtc ggtgcgccag cgcagatcaa agaaagccct gtggacgcaa
     1141 gtgtgaagga ttttttgaag gggttatagg gcgttgggta gtattgtata atcgagttgc
     1201 tttatgacat tctgatgtga tgagctgact ccaatgcctg aatttctgca attactacca
     1261 cctgaagaag cacgcaagtt attattctcc aacctccctc atgcaaagat caaacaagaa
     1321 aatatagaga ccgcctcatc gcttgggcga gtgatcgctg aagatatttc tgcgccgcat
     1381 tcactgcccg agtttttgcg ctcgactgtg gatggctatg ctgtgcgcgc acgtgatacc
     1441 tttggcgctt ctgactcgat gccagcatac ttgagcttgg tggatgaaat cccgatgggc
     1501 agtgcgccat cgcaaaacat tggctctggt caatgcgggc tgattcatac cggcggaatg
     1561 ttgccgcccg gagcagatgc agttgtgatg ttggaaaaca cacaggttgc gctgggtggg
     1621 aatcgcccaa caccgggcag tgaaatagaa atctttcgcg ctgttgccga tggtgaaaat
     1681 gtaattcaga tcggcgaaga tgtgaaatca ggctctgtta ttttgaaagg cggcactcgc
     1741 atccgtcctg cagaagtagg cggactgatg gcgttgggca tcacacatct ttcggtggca
     1801 gcgaaaccta aagtggggtt gatctcttca ggcgatgaaa tcattgaccc attcaaacaa
     1861 acgcaaccag ggcaggttcg agatatcaac gcctatacgc tcgcatcgtt ggtcaccaag
     1921 accggcggtg aacccattct gtatggcatc gttccagatg ttcgcgctga cttggaatcc
     1981 acaacccgcc gcgccttgaa tgaatgtgac gtggtggtca tcactgctgg ttcctctgcc
     2041 agcacccgcg acatgaccgc cgatgtaatt gcttcattgg gcgcgccagg cgtgttggtt
     2101 catggaatca atacccgccc cggcaagccg accatccttg gagtatgcaa tggtaaagcc
     2161 gtgattgggc tgccaggcaa ccccgtcagt gctttggtga atggatattt attcgtggtg
     2221 cctgtgattg aaagattgct cggtgaaacg cctcgtcccc gcccgatcat tgctgccaaa
     2281 ctgacggtca acctgccctc gcaagctggg cgcgaagatt ggattcccgt caaactgatt
     2341 gcgcaatcgg ggaacgctgg cagccagacc tttcaagcgg aacctatctt tggtaaaagc
     2401 aatttgatct ttactctaac cgccgccgat ggtctcttgc gcattcctcc tgatgtcact
     2461 ggatacggcg ctggagagat ggctgaagtc gttttgttct aaatttccag catcctttgc
     2521 aagcatggag acaaatcatg tctatctacc ttcatgatat tccgctgtct acagcgcaaa
     2581 atcatttccg aacatctttg gaggaagtcg gcttatggcg ggtgcttggg tcagaaacaa
     2641 ttccattgga tgaaaacgcg ctcgggcgcg tcctttcgga accgatctgg gcatccatct
     2701 cctcaccaca ttatcacgcc tccgccatgg acgggtttgc ggtcgttgcc gaaaacacct
     2761 ctggtgcggg tccctccacc ccgttgacct tccccatcga agccgagtcc gctcattatg
     2821 tggatacagg cgacccattg ccagaatggg cgaacgccgt catcccgatt gaaaatgttg
     2881 aatcgttgga tgaaaacaat accatcacct caaacattca aagacccgca tccattcgca
     2941 ttcgagcagc ggtttcgcca tggagtcaca tccgcccgtt gggagaggat attgtcgcca
     3001 cgcaattggt cttgccatcg ggtcatactc tacgccccgc ggacttgggt gcgattgctg
     3061 cctcggggca tcaaagtatt caagtagcac gcaaacctaa agttgccatt ctgccaacgg
     3121 gcacggaatt ggttccaatt ggctcgaaat taaaatcagg cgatatccta gaatacaact
     3181 ctctggtttt ggctgcacag atcaaaacca tgggcggtga cccaacgcgc tacccgatca
     3241 caaaagatga ttttgacctg atctgcaaca acgtctcaga agctgcacaa gaccacgact
     3301 tgattttgct caacgctggc tcctccgcag gcatggaaga tttttcctcc aaggtggttg
     3361 aaaagctggg gcaacttttg gtgcatggtg ttgctgtgcg ccccgggcat cctgtgattt
     3421 tgggcatcat cgatcgggat gggaaaaaag ttcccatcgt aggcgtgccg ggctatcccg
     3481 tctctgctgc actcaccacc gatatttttg ttgaaccact catcgcaaaa tggttgggac
     3541 ggcgtccact agaattgcca ac
//
LOCUS       DAOREF010000221         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16500_length_3557_cov_3.951742, whole genome shotgun sequence.
ACCESSION   DAOREF010000221 DAOREF010000000
VERSION     DAOREF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16500_length_3557_cov_3.951742"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKI33_05830"
     CDS             <1..740
                     /locus_tag="PKI33_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36558.1"
                     /translation="YIDPPFWSPDGQWIAFRTQNGMGGEEVYIIHPDGSELKNIATDL
                     PVDGRPYIMDGWYTENVIMRSALPGAQGNIYLVRAANGQSRPMFETLLTKAQFIASPD
                     AGFFAYDEYDYASQNHFVKVMEPDGANAVTLTQFTGGSIYPMIWSPDSSLLAFNYYGN
                     MTSGVSFAEVFIIPRRGGELKSVYKGNTVGRLLFSPNGKYLLIEETTSASGGHLYMVD
                     LATSSYSMLQAPGLSTDYDWYAPSWRP"
     gene            824..1849
                     /locus_tag="PKI33_05835"
     CDS             824..1849
                     /locus_tag="PKI33_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM36559.1"
                     /translation="MKLKTTLFLALFFFALLTLSSPAKAQEFGPTPTLGAPPNPADII
                     NAVNNLRLSYGLPALYAHPVLMQIAQEQASGIAAGMPGHWRPNNMTLGQWMLSLGYPL
                     SGDLTLDGYRSENWGPASTAEDAIQMWLGDDLHTNTMLSTERSDIGAGVAIADGQFIV
                     VIETALQTRSGQMQSNAVSILTGIPQTQIAYSNMETQAAINGVLPQYSIPVVLNTPFP
                     NGKVYHEVLYGQTLWSIAVAYGTTIKQIQGLNNLGDSTTVYEGQTLLVRTDATQPAPV
                     VTVTTENAVIPAEQTSTPAQTLLPLMETPQNSQNQRGPTFGVTAIILSALFLAGVFAV
                     MARKKEI"
     gene            complement(1846..2220)
                     /locus_tag="PKI33_05840"
     CDS             complement(1846..2220)
                     /locus_tag="PKI33_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNM36560.1"
                     /translation="MNPKQVLETFWEKMESNDFYAVAELLHDEFTLEWHQSGELIRGR
                     ENFAKINTAYPANGEWRFNINSIVVEGDVVVTDVSVTDGVVKDRVITFSTVRGGKIWK
                     QIEFWPEPFAAPEWRARWVEKI"
     gene            complement(2222..2617)
                     /locus_tag="PKI33_05845"
     CDS             complement(2222..2617)
                     /locus_tag="PKI33_05845"
                     /inference="COORDINATES: protein motif:HMM:NF021514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNM36561.1"
                     /translation="MNGETELGKLLKNLKPERQPGEYVFCVVNTLKHAAELNPISTFQ
                     EAEGVTVVLPKRLADENILFYSVVCAWITLTVHSSLEAVGLTAAVSSALAGANISCNV
                     VAAFHHDHIFIPVKDAEQAMEILLSLSKG"
     gene            complement(2614..>3557)
                     /locus_tag="PKI33_05850"
     CDS             complement(2614..>3557)
                     /locus_tag="PKI33_05850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36562.1"
                     /translation="SSRTLNSSSHEEAFRLFNGFYEGFPDLVLDVYGETLVIHDYSET
                     PNSVFIQELTSYIRTSLKWLRAGILKSRHGTSQKEKRGVLIFGTEPNRKIKEHGIWYS
                     IDLTMNRDASFYLDTVNLRKWLIENSRGKSVLNTFAYTGSLGVAALAGEAAKVIQTDL
                     NRQFLNLAKESCTLNGFPVEKPNFIAQDFFSVIARFKAGKQAFDCVIIDPPFFSSTSR
                     GKVDMEHESERLINKVRPLIQDGGTLIAINNAVFLSGKEYMQTLEKLCKDGYLSLREL
                     IPVPDDFMGTKRIQTPITDPAPFNHSTKIAILDVRRK"
BASE COUNT          923 a          809 c          826 g          999 t
ORIGIN      
        1 catatattga cccgcctttc tggtctccag atggtcagtg gatcgccttc cgtactcaaa
       61 atgggatggg tggcgaagaa gtttatatta tccacccaga tgggagtgag ctaaaaaaca
      121 tagccactga tttgcctgtg gatgggcgcc cttacatcat ggatggttgg tacaccgaga
      181 atgtcattat gcgttctgcg ttgcccggtg cacagggcaa tatctacctc gtccgcgctg
      241 caaacggaca gtctcgtccc atgtttgaaa ccttgcttac caaagctcaa ttcattgcat
      301 cgcctgatgc gggcttcttt gcttacgatg aatatgacta tgcatcccaa aatcattttg
      361 tgaaggtgat ggagcctgat ggagcaaatg ctgtcactct tactcaattt actggcggtt
      421 caatctaccc tatgatctgg tcgccggata gcagcctgct tgcctttaat tattacggga
      481 atatgaccag tggcgtctct tttgctgaag tattcatcat ccccagaaga ggcggggagt
      541 tgaaatctgt ttacaaaggc aatacagttg ggcgtctgct tttctctcca aacggaaaat
      601 atctcttgat tgaagaaact acatcggctt ctggcgggca cttatatatg gtggatctgg
      661 ctacttcgag ttatagcatg ttgcaggctc cagggctttc cacagattat gattggtatg
      721 cgccttcttg gagaccataa attttcaaaa gccgaagaac taaatcttcg gcttttattt
      781 tgcctgttat gattcaggta ggatgtgcaa catgatgtat gggatgaaac taaaaaccac
      841 actatttctt gctttattct tttttgcgtt attaacctta tcttctccag ccaaagcgca
      901 ggagtttgga cctactccaa cattaggtgc tccacccaat ccggcggata ttatcaatgc
      961 ggttaataat ttgcggcttt catatgggtt gccagcattg tatgcccacc ccgtgttaat
     1021 gcagattgcc caagaacaag ccagcggaat tgctgctggt atgcccggtc actggcgacc
     1081 taataacatg acgttgggac agtggatgct ctcattaggc tatccactgt ctggtgacct
     1141 gaccttggac ggctatcgct ccgaaaactg gggacccgcc tcaaccgcag aggatgcgat
     1201 tcaaatgtgg ttgggagatg acctacacac aaataccatg ctctcaacag agcgaagtga
     1261 tattggagcg ggtgtcgcca ttgcagatgg acaatttatc gtggtgatag aaacagcact
     1321 ccaaacccgc agtggacaaa tgcaatcgaa cgcggtttcg atccttacag gaatacctca
     1381 aacgcaaata gcatattcca acatggagac acaagctgcg atcaatggcg tattgccgca
     1441 atattccatt cccgtggtat tgaatactcc cttcccgaat ggcaaggtgt atcacgaagt
     1501 attgtatggg caaaccttat ggagcattgc tgtggcttac gggacgacga ttaagcaaat
     1561 tcagggacta aataatctag gagactcaac gactgtttat gaagggcaaa cactgcttgt
     1621 ccgaacagat gccacccaac ctgcacctgt tgtaaccgta acaactgaga acgcagtgat
     1681 tcccgccgag cagacatcaa cacctgcaca aacgctcctg cctttaatgg agacaccaca
     1741 aaactctcag aatcagcgtg gacccacgtt tggtgtgact gcgattattc tttctgcttt
     1801 atttcttgct ggggtgtttg ctgtaatggc gcgtaaaaaa gagatctaga ttttttccac
     1861 ccatcgggct cgccattctg gtgctgcgaa tggttcgggc cagaattcga tctgtttcca
     1921 gatctttcca cctcgaactg ttgaaaaggt gatgactcta tcttttacca ctccatctgt
     1981 gactgataca tctgttacaa caacatcccc ttcaacaacg atggaattga tattgaagcg
     2041 ccactctcca ttggctgggt atgccgtatt gatctttgca aagttttccc tgccgcggat
     2101 caattcacct gattgatgcc attcaagcgt gaattcgtca tgcaagagtt cagcaactgc
     2161 ataaaaatcg ttgctttcca tcttttccca aaatgtttct aagacctgtt taggattcat
     2221 cttagccttt tgatagggat aacagaattt ccatcgcctg ttctgcatct ttgactggga
     2281 taaatatgtg gtcatgatga aaagccgcca ccacattaca actgatattg gctcctgcta
     2341 acgcactgct gaccgccgct gtgagcccta ccgcttcgag agaagagtgg acggtgagcg
     2401 tgatccatgc acataccact gaatagaaaa gaatattttc atctgccagt cgtttaggca
     2461 aaacaacggt cacaccttca gcttcttgaa atgtagagat ggggtttagt tcagccgcat
     2521 gctttaatgt gtttacaaca cagaaaacat attcgccagg ctggcgttct ggtttcaagt
     2581 ttttcaataa ttttccgagt tctgtttcac cattcatttt cgcctcacgt ctaagatcgc
     2641 aattttagtt gaatgattaa aaggcgcagg gtcagtgata ggagtttgga tacgtttggt
     2701 tcccataaaa tcatctggaa cggggatcaa ttcacgaaga ctaagatagc cgtctttaca
     2761 aagcttttcg agggtttgca tgtattcttt gccgcttaga aaaactgcat tgtttatggc
     2821 aatgagagtg ccgccatctt gaatcaacgg gcggactttg ttgatgagcc gttcgctttc
     2881 atgctccatg tccacttttc cgcgtgatgt ggaagagaag aaaggcgggt caatgatgac
     2941 gcaatcgaag gcttgcttcc cagctttgaa acgggcaata accgagaaga agtcttgtgc
     3001 aatgaagtta ggcttttcga caggaaagcc gttcaatgtg caggattctt ttgcaagatt
     3061 caagaattga cgattaagat ccgtctggat cacttttgca gcttcgcctg cgagagcagc
     3121 aacccctaga ctgcctgtgt aagcaaaggt gtttaataca gatttcccgc gtgagttttc
     3181 aatcagccat ttccgtaagt tgacggtatc aagatagaag cttgcatcgc ggttcatggt
     3241 taagtcgatg gagtaccaga tgccgtgttc tttgatcttg cggttcggct cagtgccaaa
     3301 gatgagcaca ccgcgttttt ctttttgact tgtaccatgg cgggatttaa gaattccagc
     3361 gcgtagccac tttagagaag tgcgaatata gcttgtaagc tcttgaataa aaacagaatt
     3421 tggagtttct gaataatcgt gaataactag ggtctcacca taaacatcca acacaaggtc
     3481 tgggaagcct tcgtagaatc cgttgaagag gcgaaacgct tcttcgtggc tagaactgtt
     3541 cagggtccgg gaagagg
//
LOCUS       DAOREF010000222         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16740_length_3527_cov_4.424539, whole genome shotgun sequence.
ACCESSION   DAOREF010000222 DAOREF010000000
VERSION     DAOREF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16740_length_3527_cov_4.424539"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1918
                     /locus_tag="PKI33_05855"
     CDS             <1..1918
                     /locus_tag="PKI33_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNM36563.1"
                     /translation="IAAAGDEKKIFIWDPQTCCETVKEFEIDNKARSLKFVTLNGKEY
                     IAIASGQQITFWDSSTGKKDEALTLQISTTDETVRILSLAVAPTNDMVAAGSEDGNIT
                     VWELKTKEVKFQACSFGDPETNNEEICKVNGDGETDIRGLAFNANGTLLASGSSDHRV
                     WLWNPETGDLLARSAVSTEGGHINTVTSVTFNSKTGTIASVSWDNTVRIWEPIQNEDK
                     TWGLELVESLTGHSNSIWAAAYSPDGIHLATGSSDKTLILWNVRQASQLGVPFKKLEG
                     DAWALAVSPDKKHFAAGDTFGGIHVWDFDGNTLSNEQKIPNEGGVLALAYSNDSKWLA
                     SSGYSDNTIRVWDAATGKELWSLENAHDQEIWALAFSPDNRWLASASFDQTVKIWDTT
                     THELITEPLQHDSGVYTLAFNPNGSSLYVAGFPFNIFSYSLTDTSNIPEPQQLTGHGS
                     AVNLLASNPNYPEFIASTSDDKTLLVWNVANGEHTPPVLGLQESMEAVAFRPSGDWLA
                     SATNNNTVLLWQFDAKRCAKEWDQDTCQPQIIGSPLTGSQTAVYNVIFLSDTVMISSS
                     QGGQLIKWNLDKAYWYDVACSIVNRPLSEAEHRQYVGKQLNSKLLNAITWVSERFGND
                     VPPAPACLNIDGNN"
     gene            complement(1984..2184)
                     /gene="rpmF"
                     /locus_tag="PKI33_05860"
     CDS             complement(1984..2184)
                     /gene="rpmF"
                     /locus_tag="PKI33_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNM36564.1"
                     /translation="MTPHPKRKHSKGRRDRRRSQDALSAANLVTCSNCGNKRLPHVVC
                     PNCGYFKGREVVQVKKEKKSAE"
     gene            2313..3020
                     /locus_tag="PKI33_05865"
     CDS             2313..3020
                     /locus_tag="PKI33_05865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNM36565.1"
                     /translation="MEIQIAVAKINKYAVSESGDTLEVIERPTGGLSVVLADGQTSGR
                     GAKAVSMMVVRKVISLIAEGVRDGAAARAASDALFTERAGKVTCTLNIASVDLHSKTI
                     VLTRNNPAPMYVCRGEEIEIHNEESISLGTSRNLRPIITELNIEAGLTIIIFSDGISH
                     AGERRGTPLDVRHTLLSMLEDQDPSPQQLADTLLAEAVRLDDKRPADDISVLVLRVSP
                     RTGDDVRRMAVRLPINA"
     gene            3013..>3527
                     /locus_tag="PKI33_05870"
     CDS             3013..>3527
                     /locus_tag="PKI33_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36566.1"
                     /translation="MHKKFHFIDKHRWRHIQAGIRDTTILLREFRTPLFWFTFAVVGG
                     GILYHYLSQRLNEPVYSLTESIYIVLIATFLQPANREFPHHILLQIFHFVMPVIGLSV
                     LAQGLADFGSLFFNRKSRNKEWEMAVASTMQNHIILVGLGHLGYRVALKLHEMGEPLV
                     VIEFNADADTV"
BASE COUNT          977 a          896 c          785 g          869 t
ORIGIN      
        1 cattgcagcg gctggagatg agaaaaaaat ctttatctgg gatccacaaa cttgctgtga
       61 aaccgtaaaa gaatttgaaa ttgacaacaa agcacgttct ttaaagtttg ttaccctaaa
      121 cggaaaagaa tacatcgcca ttgcttctgg tcaacaaatc acattctggg actcatccac
      181 aggtaaaaaa gacgaagccc ttactctaca aatctctact accgatgaaa ccgttcgaat
      241 tctcagcctt gccgttgctc caacaaacga tatggtagca gctggaagtg aagacggaaa
      301 tatcacagtt tgggagctaa aaacaaaaga agttaaattc caagcctgta gtttcggcga
      361 ccctgaaacc aacaacgagg aaatttgtaa agtaaacgga gacggtgaaa cggatatccg
      421 cgggttggcg ttcaatgcaa atggaacctt acttgcatca ggaagctccg accatcgtgt
      481 ctggctctgg aatccagaaa ccggtgacct tttagctcgc tctgcggtca gcaccgaagg
      541 tggtcacatc aacacggtta cctcagtaac cttcaactcg aaaacaggaa ccattgcttc
      601 tgtaagttgg gacaataccg ttcgtatttg ggaaccaatt cagaatgaag ataaaacttg
      661 ggggcttgaa cttgtagaat ctctaacagg tcattccaat tcgatttggg cagcagcata
      721 cagcccagat ggcattcatc ttgccaccgg aagttcagat aaaaccctaa tcctttggaa
      781 tgtaagacag gcaagtcaac tcggggttcc attcaaaaaa ttggaagggg atgcctgggc
      841 attggcagtc tcgccagata aaaaacattt cgctgctgga gatacattcg gcggcatcca
      901 tgtatgggat tttgatggaa acaccttaag caatgagcaa aaaatcccga acgaaggtgg
      961 cgtattagca ctagcttaca gtaacgatag caaatggctt gcatcgtcag gctactcaga
     1021 caacaccatc cgcgtttggg atgccgcaac aggcaaagaa ttatggagcc ttgaaaatgc
     1081 ccatgatcag gaaatttggg cgctcgcctt cagcccagat aatcgttggc ttgcctctgc
     1141 cagttttgat caaaccgtaa agatatggga tacaacgact catgaattga tcacagaacc
     1201 attgcaacac gactctggag tatataccct tgctttcaat ccaaacggtt cttccttgta
     1261 tgtggctggt ttccccttca atatcttttc ctacagccta accgatactt ccaacattcc
     1321 agagcctcaa caactcactg gtcatggctc tgccgtaaat ttacttgcct ctaatcctaa
     1381 ttatcctgag ttcatcgcct ccactagcga tgacaaaacg ttgttagttt ggaatgtagc
     1441 gaatggtgaa cacactccac ccgtattagg tttgcaagaa agcatggaag ctgtcgcctt
     1501 ccgtccaagc ggagattggc tcgccagcgc aacgaacaat aacaccgtct tgctctggca
     1561 atttgatgca aaacgctgtg ccaaagaatg ggaccaagac acctgccagc ctcaaattat
     1621 tggctcgcca ctcacgggaa gccagaccgc cgtctataac gtcatcttct tatcagatac
     1681 agtcatgatc tcatccagcc aaggcggaca gttgatcaaa tggaacttag acaaagccta
     1741 ttggtatgat gttgcctgta gcattgtaaa tcgcccctta agcgaagctg aacatcgcca
     1801 atatgttggt aagcaactaa attcaaaatt attgaatgca atcacctggg tttctgaacg
     1861 ttttggaaat gatgtccccc cagcccctgc atgtttgaat attgatggaa ataactaact
     1921 gaccgaataa aaagatgctg aattaaacac aaacgggtcg tgaggttcac gacccgttgc
     1981 aatttattca gccgatttct tttctttttt aacctgaacc acttcgcgtc ctttgaaata
     2041 tccgcagttc gggcaaacga catgcggcag gcgcttgttc ccgcagttgg aacaggtgac
     2101 gaggttggcg gcgctcaagg catcctgaga gcggcggcga tcccggcgtc cctttgaatg
     2161 tttgcgcttt ggatgtgggg tcatgaaacg ttctccttta tacatacatc agacgggcgc
     2221 acgcccgtct ggattggttt tattacgaag cccgctgatt atatatgggg gaagcagtga
     2281 cgtcaaggcg aattcgatgt aaaatcctct atatggaaat ccaaatcgca gtagcaaaaa
     2341 tcaacaaata tgcggtttcc gaaagcgggg acacgctcga agtcatcgaa cgccccaccg
     2401 gcgggttatc tgtcgttttg gcagatggtc aaaccagcgg gcgtggcgcc aaagccgtct
     2461 ccatgatggt cgtccgcaaa gtgatcagcc tcattgctga aggtgtccgc gacggcgccg
     2521 ccgcccgcgc cgcctccgat gcgctattta ccgaacgagc cggtaaagtg acctgcactc
     2581 tgaatatcgc ctctgtagat ttacacagta aaaccattgt tctcacccgc aacaaccccg
     2641 ctcccatgta cgtatgtaga ggtgaggaaa ttgaaataca caacgaagaa agcatttcac
     2701 tgggtacatc gcgcaacctc cgccccatca ttaccgaatt aaacatcgaa gccgggctga
     2761 ccatcatcat cttttcagat ggcattagcc atgccggcga gaggcgcggc acccctttgg
     2821 atgtacgtca tacccttctc tccatgctag aagaccaaga tccttcccca caacaactag
     2881 cagataccct gctggctgaa gccgtccgtc tggacgataa acgcccagca gatgacatca
     2941 gtgtacttgt tctgcgtgtt tctccacgaa caggagacga cgtccgccgc atggcagtgc
     3001 gcctgcccat caatgcataa aaaatttcat ttcatcgata aacatcgttg gcgtcacatt
     3061 caggctggga tacgtgatac caccatcctt ttacgtgaat tccgcacacc tcttttttgg
     3121 ttcaccttcg ccgtagtcgg cggcggaata ctctatcact atttatcaca gcgtttgaac
     3181 gaacctgtgt acagcctcac cgagtccatc tacatcgttc tcatcgctac atttttacag
     3241 cctgccaacc gcgagtttcc gcatcacatc cttcttcaaa tttttcactt cgtgatgcca
     3301 gtcattggct taagcgtgct tgcacaaggg ttggcagatt tcggaagttt gttcttcaat
     3361 agaaaatcac gcaacaagga atgggaaatg gcagtcgcat ccactatgca aaaccacatc
     3421 atcctcgttg gtttagggca ccttggctat cgcgtcgctc taaagctaca cgaaatgggt
     3481 gaacctttgg tcgtcatcga attcaatgca gacgctgata cagtcaa
//
LOCUS       DAOREF010000223         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16741_length_3527_cov_3.947293, whole genome shotgun sequence.
ACCESSION   DAOREF010000223 DAOREF010000000
VERSION     DAOREF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16741_length_3527_cov_3.947293"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKI33_05875"
     CDS             complement(<1..680)
                     /locus_tag="PKI33_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36567.1"
                     /translation="MPLKARTTPEASISKTEKGYLMKIPAGDSSAYRFAQIDDYFGLP
                     RKKFPHHALTLSLRARTSSSAIPGTWGFGLWNDPFGMSLGFGANRFRLPTLPNAAWFF
                     GASSENHLSFSDKPAQGFLAQSFCSPKFHASLIPAGMLLPFSPKMTRRMLSKVIAEDS
                     SALRVDVTQWHGYRLEWRSNRVAWYVDEAQVFESSVSPNPPLGLVIWIDNQYAAFKPE
                     GKISFGVL"
     gene            complement(685..1572)
                     /locus_tag="PKI33_05880"
     CDS             complement(685..1572)
                     /locus_tag="PKI33_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNM36568.1"
                     /translation="MPKSKVIPYLEALFAVLVWGGTFIATKIALSEVSPATIVWIRFG
                     MGTVILGIVVVARKQFALPEKSEWLYFAMLGFLGVTFHQILQATGLQTAKATTTAWIV
                     ATTPVFIAILGWVALKEKLNGLQILGIVLAGFGVLLIVSKGNIGALFTGEEGTFGDFL
                     VLISSVNWAVYTILSRRELARHPAARMMFFVMLIGWLLVNVWIFGFGPGVSEIPQLTS
                     SGWVAILILGIFGSGLAYIAFYDALQEIPASQLGAFFNVEPLVTTILASFMINEAITA
                     ITLIGGAIIILGIWLVNRK"
     gene            1802..2449
                     /locus_tag="PKI33_05885"
     CDS             1802..2449
                     /locus_tag="PKI33_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM36569.1"
                     /translation="MEITWYGHSCFRLTERNYATVVTDPFDHKAVGYSALKLKADIVT
                     VSHDAPGHANLEAVKGSEHVIDGAGEFEIGGVFITAVQTAGGGKKSKDKTRNTLYVFD
                     YEGITVAHLGDLQEVPTQSEVEALGTVNILLIPVGGGSSLNAAKAAEVVSVIEPNLVI
                     PMHYSTDAAKIKLDPLNKFLKEMGLGKVESLPSLKATRSSLPQETKVVVLDYQQG"
     gene            2451..2753
                     /locus_tag="PKI33_05890"
     CDS             2451..2753
                     /locus_tag="PKI33_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36570.1"
                     /translation="MAVKVIMTWDIAAERDQEYFEFIIGEFVPGVQRLGLQPAEAWAT
                     LYGSYPQIQVGLLATDAPEARRVLASAEWAMLQDRLFGYVKNYSYKVVPVRTGFQF"
     gene            2816..>3527
                     /locus_tag="PKI33_05895"
     CDS             2816..>3527
                     /locus_tag="PKI33_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36571.1"
                     /translation="MPNKFSKILPWFFFAAAFQSLLAVAALLRIPSEGLSLARLALLG
                     VIGGIFLLSIGLGLARRNLLRFEWLSTTPVIISSALLSLTFSLLLFLLRYFNPERFLP
                     YYERMSPLLWLLFLLAAEAALCFLLLKNGFHPQSLTNYSSAFRASVIPLLVLFTIFLF
                     VAITKIGVTPDTGYWGEPGVAIQGWHFVIALLLGCTVLILSSSNSSLFIRYLPFVLYA
                     IAAILWLSVPLETLANSFY"
BASE COUNT          887 a          863 c          861 g          916 t
ORIGIN      
        1 tctaaaaccc caaatgaaat tttgccttca ggcttgaatg ccgcatactg attatcgatc
       61 cagatgacca accctaaggg cggattcgga cttactgacg actcgaacac ttgggcttca
      121 tctacatacc aagctacccg gttcgatctc cactcgagcc tgtatccatg ccattgagtg
      181 acatccaccc tcagcgcgga cgagtcctcg gcgatgacct tgctcaacat acggcgggtc
      241 attttgggtg agaaagggag gagcatccct gcagggatga gggatgcgtg gaattttggc
      301 gagcaaaaac tttgtgctag aaatccttgt gcaggtttat cgctgaacga gagatgattt
      361 tctgatgaag ccccaaagaa ccaagccgca tttggcaatg tgggtagacg gaatcgattc
      421 gcgccaaagc ctagtgacat gccgaaggga tcattccaca agccgaagcc ccaggttcca
      481 gggatggcag agcttgaggt ccgcgcacgc aggctcaagg tcagagcatg atgcgggaat
      541 tttttccgag gtagaccaaa gtaatcatcg atctgtgcaa aacgataggc agacgaatct
      601 ccggctggga ttttcatcaa atagcctttt tcggtctttg agatgctagc ttcaggggta
      661 gtgcgtgctt ttaatggcat ttgattattt ccgatttacc aaccaaatac ctaatatgat
      721 aattgcgccg ccgatgagag tgattgctgt gatggcttcg ttgatcatga atgaagctag
      781 aatggtagtg accaacggtt cgacattgaa gaatgctcca agttgtgagg cggggatttc
      841 ttgcaaggca tcatagaatg caatgtacgc caaccctgaa ccgaagatac caagaatcag
      901 aatggcaacc cagcccgaag acgtgagctg cggaatttca ctcacacccg gaccaaaacc
      961 aaaaatccac acattgacca aaagccagcc gatcagcatg acgaagaaca tcatccgtgc
     1021 cgcagggtga cgtgctagct cacggcgcga gaggatggtg tacaccgccc agttgaccga
     1081 gctgatgagg acgaggaaat caccgaaggt cccttcttcg ccagtgaaaa gcgcaccgat
     1141 attgccctta ctgacgatga gcaaaacccc aaacccggca agaacaatac cgagaatttg
     1201 caacccgttc agtttttctt tgagtgctac ccaaccgaga atggcaatga aaacgggcgt
     1261 ggttgccacg atccatgcag tggtggtggc tttggcggtt tgtagccccg tagcttgcag
     1321 gatttgatga aaggtgacac ccagaaaacc gagcatggca aaatatagcc actcgctttt
     1381 ttcaggcagg gcaaattgct tgcgggcgac aacgacaata ccgaggatga ctgttcccat
     1441 cccaaaacgg atccacacga tggtagcagg ggagacttcg ctgagcgcga tcttggtggc
     1501 gataaacgtc ccaccccaaa cgaggacggc aaataaagct tcgagatagg gaatgacttt
     1561 agattttggc ataaggtttc ctgaggaaag agttggagaa ttataattgt tgtttactac
     1621 actactttaa ggggtatttt tcctaagagg aattcaccat ttgggtaggg attttgaagg
     1681 tgttgtgatg gtcggaaaat agtcctgtca aaaactaagc ctatttgact attagacgac
     1741 caaacttcat tttgtccgtg tacccatcgg gtataatatc tccctcggag gcactagcta
     1801 catggaaatt acatggtatg gacattcttg ttttagactg acagaacgca attacgcgac
     1861 tgttgtcacc gacccgttcg atcacaaggc ggtgggctac agtgcgctca aactaaaagc
     1921 ggatattgtc accgtcagcc acgatgcacc cggtcacgcc aatttagagg cagtgaaggg
     1981 tagtgagcat gtcatcgacg gcgcgggcga atttgagatc ggcggtgtat ttattacagc
     2041 ggttcaaact gccggaggcg gcaagaaaag taaggataag acccgcaaca ccctctatgt
     2101 gttcgattac gaaggtatca ctgtggcaca ccttggcgac ttgcaggaag tgcccactca
     2161 aagcgaagta gaagccttgg gaacagtgaa cattctcctc atcccagttg gaggcggaag
     2221 cagtctcaat gccgccaaag ctgcggaagt ggtcagcgtt atcgagccga atcttgtcat
     2281 ccccatgcat tactccaccg acgccgccaa gatcaagctc gacccattga acaaatttct
     2341 caaagaaatg ggtctcggca aggttgagtc tctgccttct ttgaaagcca cccgttcttc
     2401 attaccgcaa gaaaccaagg tggttgtgct cgattaccaa caaggatagt atggcagtta
     2461 aagtcatcat gacctgggat atcgccgctg aacgcgacca ggaatatttt gaattcatca
     2521 tcggtgaatt cgtacccggt gtgcaacggc ttggcttaca gcccgccgaa gcttgggcta
     2581 ccctttacgg ttcatacccg caaattcaag taggcttgct tgccactgat gcacccgaag
     2641 cgcgccgtgt gttggcatct gctgagtggg cgatgttgca agaccgtttg ttcggctatg
     2701 ttaaaaacta ttcttataaa gttgtacctg taagaactgg gtttcaattt tagataccaa
     2761 tacaccatca ttgcgaggag ggtgatagct ctcccgcaat gacggaatag gtcacatgcc
     2821 aaataaattt tcaaaaattc tgccgtggtt tttctttgcc gcggcatttc agtctctact
     2881 cgccgtcgct gcactgttgc gaattccatc tgaaggcttg tcgctggcgc gccttgcctt
     2941 gcttggagta ataggaggca tctttttatt aagcatagga ttgggacttg cacgccgcaa
     3001 ccttttacgc ttcgaatggc tttcgacaac accggttatt atctcgtccg cgcttctctc
     3061 gctgaccttt agcctgcttc tgttcctttt acgttatttc aaccctgagc gttttctacc
     3121 ctactacgag cggatgagtc cgctgctttg gcttttattc ttgcttgccg ctgaagccgc
     3181 tctctgcttc cttctcctca aaaacggatt ccatccgcaa agcctcacaa actatagctc
     3241 ggcatttcgc gcttctgtta ttccgctttt agtccttttt actatctttc ttttcgtagc
     3301 tattaccaag atcggcgtca ccccagatac cggctactgg ggcgaacctg gtgtagccat
     3361 tcaaggctgg cattttgtta ttgctcttct gttgggatgc actgttctaa tactttcctc
     3421 ctctaactca tcgcttttta ttcgttactt gccttttgtt ctttatgcta ttgctgccat
     3481 cctttggtta agtgttccgc ttgaaacgtt ggcaaatagc ttttatg
//
LOCUS       DAOREF010000224         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16857_length_3513_cov_4.121457, whole genome shotgun sequence.
ACCESSION   DAOREF010000224 DAOREF010000000
VERSION     DAOREF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16857_length_3513_cov_4.121457"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKI33_05900"
     CDS             complement(<1..517)
                     /locus_tag="PKI33_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36572.1"
                     /translation="MRWNKVSLSFLALFLFFLLFFSLYLPLRPVGQILDRGWTAEYKG
                     VQTSNVQIPYIQRSSEEADILTLTTTLTDIQGNSLIFRPGGYAFSVFLNGQQIAGVGD
                     LEEPTANVWNSIQLIPLTNLNSGTNTIQIKLSSANRSVGLGTPPYIDNLKNSITRVAF
                     INWFANDFLILN"
     gene            complement(684..2126)
                     /locus_tag="PKI33_05905"
     CDS             complement(684..2126)
                     /locus_tag="PKI33_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM36573.1"
                     /translation="MQAVPSEFLAIDRKERANRPFFDLDLRAADDRKCDFGAVVIEFD
                     PERAMVEAARCIHCPDPAPCMVACPTANDIPSAMWLIEQGKFVEAAGIYHKTSSLPEV
                     CGRVCPHESLCQGSCVLNKHHTPVLCGELEAFTVDYERRTQGYLIPLTIPSGKKVAVI
                     GAGPAGLGCADILLQKGHEVTIFDAKPAPGGLLVYGIPNFKLAKDVWGEKWEEFERAG
                     AKFVPNTYIGKDKTIDQLFAEGFDAVFIGVGSEIDAKMEEAPGTDLPGVYEATDFLIR
                     GNIPEDQLPSDMKKPLEIGKRVVVVGGGDTASDCLRTAIRLGAEEVTCLYRRTEKEMP
                     GGKKDRKMARDEGAKYRFLTQPVKFIAGADGKLAAVECIEMKLGEPDAKGRRKPVPVE
                     GSNFSVAADTAILALGYWPDPVIGKSTPDLETYNWGLVSVKDKKTGATSREGVFAGGD
                     CATGPDLVVTAMVGGRKAGFAIDEYLKNKK"
     gene            2448..>3513
                     /locus_tag="PKI33_05910"
     CDS             2448..>3513
                     /locus_tag="PKI33_05910"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNM36574.1"
                     /translation="MRQFFSRFSRRTWIIIAVVAVVLICVGVSIFRPKNEVAVFETVA
                     VERGDLAAYVGATGSVRARQTATLTWQTSGIVGEVNAVIGDRVERGFVLSGLDKATLS
                     QSIILAEADLVSAQKALDDLLQSNTSLLEAEKAVDKAEESYQKAYNWRMDLNGKVDIK
                     EFYYDQFGELKVKQYKGYASAETIADADRDLALAESKLEDARREYERLQKGPDSDEVA
                     AARARVAAAEATLNIAKIIAPFDGTITQSNPVVGDQVNPGTVGFRIDDLSSLLVDVQV
                     SEVDINNVAVGQDATLSFDAILGKSYNGKVVEVGQAGDTVQGVVSFTVTVELTDADEQ
                     VKPGMTTAVNIITEEVKNTLL"
BASE COUNT          931 a          845 c          820 g          917 t
ORIGIN      
        1 gattgagaat taagaagtcg tttgcgaacc aattaataaa tgcgactctc gtaatgctat
       61 ttttcaggtt atctatataa ggcggagtgc ctaaaccaac ggaccgatta gcactactga
      121 gtttgatttg tatagtattg gtgccagaat ttaagtttgt aagtggaatg agctgtatgg
      181 agttccatac attagccgtc ggttcttcaa ggtctccaac tcctgcgatc tgttgaccat
      241 ttaagaaaac agaaaaagca taccccccag ggcggaaaat aaggctattt ccttgaatat
      301 cggtcagagt tgtagtcagg gtgagaatat cagcttcttc actactacgt tgaatgtatg
      361 ggatttgaac attagaagtt tgaacccctt tgtattcagc agtccaaccc cggtctagaa
      421 tttgcccaac cgggcgcaat ggcagataga gcgaaaagaa aagaagaaaa aaaagaaata
      481 gggctagaaa agatagagag actttgttcc aacgcattat tgtcaatttc caaatttgag
      541 atttgtctta gacttgcctg gaatacatac tctttttatt gcctaaaatt atacgccctc
      601 tcgcttttat atattattaa ggcgcacggc agagctggtg actctgccgt gctaacgacc
      661 tgctgccctc aacaggtaat tcgctatttc ttgttcttca agtactcatc aatggcaaac
      721 ccggctttgc gtccgccaac catggcagtt acaacgaggt ctggtccagt tgcgcagtcg
      781 ccaccagcaa acacgccttc acggctggtc gcgccggtct ttttgtcttt tacggagacc
      841 aagccccaat tgtaggtttc aaggtctggg gtggatttgc caattacggg gtccggccag
      901 tagccgagcg ccagaatggc ggtgtccgct gcgacactaa agttggaacc ttctactggt
      961 acaggtttgc ggcggccttt tgcatcgggc tcgccaagtt tcatttcaat gcattccact
     1021 gcggctaatt ttccatccgc accggcaatg aacttcacag gttgggtgag gaagcgatac
     1081 tttgcccctt catctcgtgc cattttgcga tctttcttcc cgccgggcat ttctttttca
     1141 gtacggcgat acagacaggt tacttcttca gcgccaagac gaatcgcggt tcggagacag
     1201 tccgaagctg tatccccgcc accaaccacc accacccgtt taccgatttc gagcggcttc
     1261 ttcatatcac tgggaagttg atcttctgga atattcccgc gaatgagaaa atctgtcgct
     1321 tcatacactc cgggcaggtc ggtgccgggc gcttcttcca tctttgcgtc aatttcagaa
     1381 ccgaccccaa taaagaccgc gtcaaaacct tcggcaaata actgatcgat ggttttgtct
     1441 tttccaatat aagtattggg gacgaacttt gcaccggcgc gttcaaactc ttcccacttc
     1501 tcgccccata catctttagc aagtttaaag tttggaatgc catacaccag caagccaccg
     1561 ggggctggct tggcatcgaa aattgttacc tcgtgtccct tttgtagcaa aatatcggca
     1621 caacccaaac cggcaggtcc tgccccgatc actgctactt ttttgccaga agggattgtc
     1681 aacggaatga gatacccctg tgtacggcgt tcataatcta ccgtaaaggc ttcaagctca
     1741 ccgcacagca cgggagtgtg atgcttattc agcacgcaag acccttggca taatgactcg
     1801 tgcgggcaaa cgcgcccaca aacttctggt agggagctgg tcttgtgata aataccggcc
     1861 gcctctacaa acttaccctg ctcgattagc cacatagcag agggaatatc attcgctgtg
     1921 gggcacgcca ccatgcacgg agccgggtct gggcagtgga tgcaacgagc cgcttccacc
     1981 atggcgcgct ccgggtcaaa ttcgatcact acagccccaa agtcacactt ccgatcgtct
     2041 gcggctctca aatctaaatc aaaaaaggga cggttcgccc gttccttgcg gtcaattgcc
     2101 aaaaattcgc tgggtactgc ctgcatgcag aatgcctcca aaggggagaa agatacaaat
     2161 aaagcggttt cccgaataat aaccaaataa atcggataga actaggttta aatgtcacca
     2221 aaccaaaaga caatcgtcat aaatttatga gtctttgatt aataggtatc gctcgcttgt
     2281 aattttgaaa tgtcgctttt ttgactgtca aattacaaga taaagagata gaatcgatta
     2341 gttaaaatct gcacaaactt tccacaatct tttgcgtaga gtgtgatata tcaatgtaag
     2401 aaaacagcac tgcacgctta ctgtaaccta attggaggaa ctcccgaatg cgtcaatttt
     2461 tttctagatt ttcccgacga acatggatca ttatcgcggt tgtagcggtg gtcctaattt
     2521 gcgtcggtgt ttctattttc cgcccgaaga acgaagtagc cgtttttgaa actgttgcag
     2581 tagagcgcgg tgacttggca gcgtatgtag gcgccactgg aagtgttcgt gcccgacaaa
     2641 ctgccaccct tacctggcag acgagtggca ttgtagggga agtgaatgct gtgattggag
     2701 accgcgtcga acgtggattt gtcctgtccg gactcgacaa agcgactctc agccagagca
     2761 ttatcctcgc cgaagctgac ctggtcagcg cacaaaaagc cttagatgat ttacttcagt
     2821 cgaatacgtc tttgctagaa gctgaaaaag ctgttgataa agccgaagaa tcctaccaaa
     2881 aagcctataa ctggcgcatg gatctcaatg gcaaagtaga tattaaagaa ttttattacg
     2941 accaatttgg cgaattgaag gtcaagcaat acaagggcta tgccagtgct gaaaccattg
     3001 cagatgccga ccgtgacctt gccttggctg aatcaaaatt ggaagatgct cgccgtgaat
     3061 atgaacgcct ccaaaaggga ccggactctg atgaagtagc tgcggctcgt gcccgtgttg
     3121 ctgctgctga agccacattg aatatagcaa agattatcgc accgtttgat ggcacgatca
     3181 cccaatctaa tccagtggta ggtgatcagg taaaccccgg cactgtaggc ttccgcattg
     3241 atgacctctc cagcttgttg gtcgatgtac aagtctctga agtggatatt aacaatgttg
     3301 ctgttgggca agatgccacc ctttcttttg atgcgattct tggaaaatcc tataacggca
     3361 aagtcgttga agtgggtcag gctggcgata cggttcaagg cgttgttagc tttacggtca
     3421 ctgttgaatt gaccgatgca gatgaacaag tcaagcccgg tatgaccacc gctgtaaaca
     3481 tcatcactga agaagtgaaa aatacgcttc tca
//
LOCUS       DAOREF010000225         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16859_length_3513_cov_2.909196, whole genome shotgun sequence.
ACCESSION   DAOREF010000225 DAOREF010000000
VERSION     DAOREF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16859_length_3513_cov_2.909196"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PKI33_05915"
     CDS             complement(<1..1211)
                     /locus_tag="PKI33_05915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM36575.1"
                     /translation="MSTSSVTIKTLLNQYRIEEFITLTPFGDLYRAIDERNGKSVALT
                     ILSKAIASDPETLKELEANNVRLQTLHHPNLNKHLGLQKTPEQAFLLEDWVDGPSLKD
                     LRGKGRLGANEALFITKYVCAGLEVLHKQNLLHLHLAPEFIRINKRGEVVLCGLAGAK
                     PVNTKAEIKFSKYQAFYKSPEQLREEKLTTAADIYSLAVILYELTTSFWINGKAAPKT
                     SDAIRKTHLDVTPPAPASINHNLPDNFSRMILWALRKNPEDRLKTTTELASSLALGAQ
                     TSLDKVPLHTTPEFAPVISAALNTWTFLPPPKPTSVSADAIPLEERLAAISGPKPKST
                     RKLGVVPVLLFLVLAGFISLFFFIRPAAETVLPTPIVYTSIAVNYTPPPTSTVTPRPT
                     LTNGGRIVFTCT"
     gene            complement(1239..1442)
                     /locus_tag="PKI33_05920"
     CDS             complement(1239..1442)
                     /locus_tag="PKI33_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36576.1"
                     /translation="MISKLLDDLSNYLAHRKGLIPIIGLLLIILNLILQFIFPGSLIV
                     TTNLFLHIGLIIAIFGLMLAWAL"
     gene            complement(1439..2809)
                     /gene="hflX"
                     /locus_tag="PKI33_05925"
     CDS             complement(1439..2809)
                     /gene="hflX"
                     /locus_tag="PKI33_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNM36577.1"
                     /translation="MAKKLPQPTTPPRERAFLVGADLHDNHSILSLEDSLAELALLAD
                     TAGVDVVGELTQKLNRPHVETYIGPGKVEELKALAEETLCEVVIFDDELLPRHQRELE
                     KALGKNIRVIDRTALILDIFAQHAHTKEGMLQVELAQYEYNLPRLTRAWTHLERQAGG
                     GGGRSGSTGGVGLRGPGETQLEVDRRAIRRRITHIKKELEKVEEHRTRYRAQRRRTRI
                     PTVALVGYTNAGKSTLLNRLAKADVYVADQLFATLDPTTRRVQVSGENTVLFTDTVGF
                     IQKLPTALVVAFHATLEEIAEADLLLHVVDISHPNALGQYQAVLETLKEIDADHIPMI
                     TALNKMDQLRDPQNAERVLENFPKSVAISALKGTGIDNLLMLVQEELFEAFEPIGVRL
                     PYKEGSLISLFHELGQVERVEHERGGVIIHGSIPGRLLAQFAPWKIKPNGNTSHTLEE
                     MDLEEE"
     gene            complement(2769..>3513)
                     /locus_tag="PKI33_05930"
     CDS             complement(2769..>3513)
                     /locus_tag="PKI33_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM36578.1"
                     /translation="LVQNSEQAVMKNYLSGVNTGIQRLRDIIDDMIDVSLIDNQLLTL
                     NIQPMWTTHLLNLVRNEFKKPIAERNQTLNVYDFDGSDLMIYGDSERLYQALQNVVSN
                     AIKYTPDKGIITIDGRLLPGFIEITVKDTGIGISPENQSLIFDKFGQLGRTDLHSSGK
                     TKFKGGGPGLGLSITRGIIEAHGGTIWVESTGYDEVQLPGSIFHILLPTRTEPNNPVM
                     LKFFGNNETKMTETESSGKETPPTDNPSA"
BASE COUNT          826 a          757 c          997 g          933 t
ORIGIN      
        1 gtgcaggtga aaacgatgcg cccgccattg gtcaacgtgg ggcggggtgt gactgtggaa
       61 gtgggcggtg gggtgtagtt cacggcaatg gatgtgtaaa cgatgggggt ggggaggacg
      121 gtctctgcgg cagggcggat gaagaaaaat agagagataa aacctgctaa gacgaggaaa
      181 agtaaaacgg gaaccacgcc gagcttgcga gtagacttgg gctttggacc agagatggcg
      241 gcaaggcgtt cttctagtgg aatggcatct gcgctgacgg aggtgggctt gggtggcgga
      301 agaaaggtcc acgtgttgag agcggctgag ataacgggag caaattcggg tgtagtatga
      361 agcggaactt tatccaatga agtttgcgcc cctaatgcaa gtgaagatgc taattcagtc
      421 gtcgttttga gtctgtcttc tgggtttttg cgcaacgccc aaaggatcat gcgtgaaaag
      481 ttatcaggta ggttgtggtt gatgctggca ggggcaggcg gtgtcacgtc cagatgtgtt
      541 ttgcgaatgg catcactggt tttcggagcg gctttgccgt tgatccagaa tgaggttgtt
      601 agttcgtaaa ggatgacggc gagtgagtag atatcggcgg cggtggtgag tttttcttcg
      661 cgcagttgtt cgggtgattt atagaacgcc tgatatttgc tgaacttgat ttcggctttg
      721 gtattgaccg gttttgcgcc tgccaacccg cagagaacga cctcaccgcg tttgttgatg
      781 cgaatgaact ctggtgcaag atgcaagtgc aataggttct gtttgtgaag tacttcgagc
      841 ccggcgcaga catattttgt gatgaacaag gcttcgtttg cacccagcct gcctttgcca
      901 cgcaggtctt ttagtgaagg tccatccacc caatcttcca gcagaaatgc ttgttcggga
      961 gtcttttgca gacccagatg tttgttgagg tttgggtggt gcaaagtttg taaacgaaca
     1021 ttgttggctt caagttcttt gagtgtttcc gggtcgcttg caattgcttt ggatagaatc
     1081 gtaagcgcaa cggacttccc gttgcgctcg tcgatggcac gatataaatc tccaaagggg
     1141 gttaaggtga tgaactcttc aattcgatat tggttgagca gggttttgat cgtaacggaa
     1201 gaggttgaca ttgaagtacg tcgcgatcag gcgtagattt agagcgccca tgccaacatc
     1261 aaaccgaaga tggcaataat caacccgata tgcaggaaaa ggtttgtggt gacgattaga
     1321 gaaccgggaa aaataaattg taagatcaga ttgaggatga tcaaaagcaa cccaataatg
     1381 ggaattaacc ctttgcgatg ggcaagataa ttggaaagat catcgagtaa ttttgagatc
     1441 attcttcctc caaatccatt tcttctaaag tgtgtgaggt attgccattg ggctttatct
     1501 tccacggtgc gaattgtgct aaaagcctgc cgggaatgct gccatgaata atgacaccac
     1561 cgcgttcatg ctctacgcgt tccacttgac ctaactcgtg gaataaagaa atgagagaac
     1621 cttctttata gggcaacctc acaccaatcg gctcaaaagc ttcaaataat tcttcctgta
     1681 ccagcatgag taagttatct atgccggtgc ctttcagtgc ggagatagcc acagacttcg
     1741 gaaagttttc caaaacacgt tcagcgtttt gtgggtctct caactggtcc attttgttca
     1801 atgcggtgat catggggata tgatcggcat cgatctcttt caaggtttcg agcacagctt
     1861 gatattgccc aagggcgttg ggatgagaaa tatccacaac gtgtaagagc agatctgctt
     1921 cggcgatttc ttcgagggtg gcgtgaaagg caaccaccaa cgcggtaggc agtttttgaa
     1981 tgaagcccac tgtgtcggtg aacaatacgg tattttcacc agagacttgc acacggcgtg
     2041 ttgtggggtc gagggtggcg aataattgat ctgccacgta cacgtctgct ttggcgaggc
     2101 gattcaacag cgtggatttg cctgcgttgg tgtacccaac caaggcgacg gtggggatgc
     2161 gggtgcgtcg acgttgagcg cgataacgtg tgcgatgctc ttccaccttt tccaattctt
     2221 ttttgatatg ggtgatgcgt ctacgaatag cacggcgatc tacctcgagc tgcgtttcac
     2281 caggaccgcg caaacccacg ccacctgtcg agccggaacg tccgccgcca ccacctgcct
     2341 gacgttcaag atgtgtccat gcgcgggtga ggcgtggcaa gttgtattcg tattgagcaa
     2401 gctccacttg caacatgcct tcttttgtgt gcgcatgttg tgcaaagata tcgaggatca
     2461 gagccgttcg gtcaatgacg cggatgtttt tgccaagcgc cttttccaat tcgcgttggt
     2521 gacgtggcag aagttcatca tcgaaaataa ccacttcaca caaagtttct tctgcgagcg
     2581 ctttgagttc ttccaccttg ccgggaccga tatacgtctc aacgtgcgga cggttgagtt
     2641 tttgagtcag ttcaccgacc acgtctacac cggcagtatc tgccagcaag gcaagttcag
     2701 caagagagtc ttcgagagaa aggatggaat gattatcgtg cagatccgcg cccactaaaa
     2761 aggcgcgttc acgcggaggg gttgtcggtt gggggagttt ctttgccact tgattccgtt
     2821 tctgtcattt tcgtttcgtt attaccaaaa aacttaagca tcaccgggtt gttcggttcg
     2881 gtgcgggtcg ggagcaggat atgaaaaatg gaacccggta attgtacttc gtcgtatcct
     2941 gtcgattcaa cccagatggt gccgccatgc gcttcaataa tgccgcgggt gatggataga
     3001 ccgagaccgg gtcctccgcc tttgaacttg gtcttaccac tcgagtgtag gtccgtccgc
     3061 ccgagctgac cgaacttatc aaagatcaaa gactggtttt ctggtgagat cccaataccg
     3121 gtatctttga cggtgatctc aataaagccg ggaagtaatc gtccgtcaat cgtgataatc
     3181 cctttatcgg gtgtgtactt aatggcgttg gagacgacat tttgcaaggc ttgatacaag
     3241 cgttctgaat cgccgtagat catcaaatcg ctgccgtcga agtcgtaaac gttcagggtc
     3301 tggttacgtt cggcgatggg cttcttgaac tcattgcgga caaggtttag cagatgggtc
     3361 gtccacatgg gttggatgtt gagggtgagc agttggttgt cgatcaacga gacgtcgatc
     3421 atgtcatcga taatgtcgcg caaacgctga atgccagtgt tgactccgct taggtagttt
     3481 ttcatcactg cctgctcaga gttttgtacc aag
//
LOCUS       DAOREF010000226         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16958_length_3499_cov_4.242451, whole genome shotgun sequence.
ACCESSION   DAOREF010000226 DAOREF010000000
VERSION     DAOREF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16958_length_3499_cov_4.242451"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..813
                     /locus_tag="PKI33_05935"
     CDS             256..813
                     /locus_tag="PKI33_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNM36579.1"
                     /translation="MRKILVSMFMALDGVVEAPNQWSFQFGSKEQQKYKYDELFTCDA
                     LLLGRETYQGFAAAWPNMPGTGDYGVRMNSIPKYVVSTTLQEAEWNNSKIIKNNISEE
                     LTTLKQQTGQDIFIFGSSTLVNSLMQYDLIDEYRLMVFPVIVGSGKRLFQDMNSKTTL
                     KLIKTETFSSGVVVLSYQPDRSEPK"
     gene            1163..1942
                     /locus_tag="PKI33_05940"
     CDS             1163..1942
                     /locus_tag="PKI33_05940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36580.1"
                     /translation="MGTASVQGQIWGTRAKDWADAQEGVAIPLFEAVLHETEVGENTS
                     VLDIGCGSGIFCEMAAKRGARVSGLDASEHLVAIAHERVQSGEFRTGEMEELPYDNQT
                     FDVVTGFSSFQFAANPVNALKEASRVSKTGTVVIAVFGKPEESESTAYIKAMGALLPP
                     PPPGAPGPFALSADGALEALTIKAGLIPSTVKAVNCPWDYPDEETALRGLMSSGPAIR
                     AIQEKGEEIVRDHILQVIAPFKTRSGGYYLTNSFHYLIAKT"
     assembly_gap    2203..2302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2303..2563
                     /locus_tag="PKI33_05945"
     CDS             <2303..2563
                     /locus_tag="PKI33_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36581.1"
                     /translation="FLIVSQFLKIKPYKLVSIRGLARLEKGHGNRFSHVYYDLHINNQ
                     QFDGDSTLNKVIVQGAEYIVYYLESNAEIMSIEKVSNEMSVA"
     gene            2796..3365
                     /locus_tag="PKI33_05950"
     CDS             2796..3365
                     /locus_tag="PKI33_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36582.1"
                     /translation="MNIKLIIKTFFILLVSLIISSCGTRPLLLQTPTPTTLLTAISQP
                     TKIPTPFVATFDDNLCEIKGSFILDGELCYINDVCTNGTADSRNIEGSPSYALGLLGY
                     YANGDSQYLSIWIKHSLSPKPNEMQTLPNHFEISNIKIYTSNNVVLQSDLEPVRLKVH
                     VIPAQITEPGADAYPLLSCDYVVEEIQLR"
BASE COUNT         1084 a          710 c          722 g          883 t
ORIGIN      
        1 aacaagatag attttagtca gtagtaaaat gtgaaagata aaatcttgcg ttatgcgaaa
       61 tgcgcccaac aaagcgtgca gcaagatact acctgacttt ttttgattgg acggcttcgc
      121 tgtccccgcc ccagcgcagg tatcccctat cggggacgag ccgcaatccg tttggcgttt
      181 ggcacaagtt tacatattgg ttttatgcct aaatcaccaa aacttatctc ttatcaataa
      241 aaaaggaaaa attcaatgag aaaaattttg gtatccatgt tcatggcact agacggtgtt
      301 gtagaagccc caaatcaatg gtctttccag tttgggagca aagaacagca gaagtataaa
      361 tatgatgaac tctttacatg tgacgctctc cttttggggc gagaaaccta tcaagggttt
      421 gcagccgctt ggccaaacat gcctggcaca ggagattatg gcgtaagaat gaacagcatc
      481 cccaaatatg ttgtttcaac aacccttcag gaagccgaat ggaacaattc aaagataata
      541 aaaaacaata tttctgaaga actaacaaca ttaaaacagc aaacgggtca ggatattttt
      601 atttttggaa gtagtactct tgtcaattca ctaatgcaat acgacctcat tgatgagtat
      661 agactgatgg tttttcccgt tatagtggga agcggaaagc gactcttcca agatatgaat
      721 agtaagacaa ctctaaaact aatcaagaca gagaccttca gttcaggtgt tgttgttctt
      781 tcctaccagc ctgacagaag tgaacccaaa tagagattag cttcccgcca aaaaacgccc
      841 aacaaagcgt gcagcaagat actatctggt ttttctggct ggaaggcttt gttcaaatga
      901 gctatgtatc tcctcaccta acaggctatg actcattcag acacaggtca taacctgttg
      961 ggcacaatat aacactcggg agttctcaat ctcccgagtg tttctattta tatgggtgta
     1021 agtccgcgct tcaatatggt attgagagaa tgggtgaaaa caaagtccac aatttcctgt
     1081 cagggtatac tagcaaccat aaagcagtaa gggtcaacag ccgtaggatt taaaacacca
     1141 gtttctccaa ggagtaaact ttatgggaac agcaagtgta caaggacaaa tttggggaac
     1201 acgagctaaa gattgggcag atgcgcaaga aggtgtagcc attcctctct ttgaggcggt
     1261 tttacacgaa acggaggtag gggaaaacac atccgttcta gatattgggt gtggctcagg
     1321 tattttttgt gaaatggcag caaaacgcgg tgcgcgggtt agtggactgg acgcatcgga
     1381 gcatctcgta gccattgctc acgagcgtgt acaatctggc gaattccgaa caggggaaat
     1441 ggaagaattg ccctatgaca atcagacatt tgatgtagta acaggattta gctcatttca
     1501 attcgcagcc aatcccgtca acgcattgaa agaagcaagc cgcgtatcaa aaactggaac
     1561 ggttgtaatc gctgtatttg ggaaaccgga agagagcgag tcgacagctt atattaaagc
     1621 aatgggggca ttattacctc ccccaccacc aggggcgccg ggtccatttg cgctttctgc
     1681 tgatggtgcc ttagaagccc ttaccatcaa agcgggcttg atcccttcaa cggtaaaggc
     1741 tgtcaattgt ccgtgggatt atcctgacga agagactgct ctgcgcggtt taatgtcatc
     1801 gggtccagca atacgagcca ttcaagaaaa aggcgaagaa atagtacgcg accatatatt
     1861 gcaagttatc gctccattta aaacaaggtc aggtggatat tatttgacaa atagtttcca
     1921 ctatctcatt gcaaaaactt aataataaag acagcatatc atttctggct ggacggacaa
     1981 gctaagtccg cgcgccggat caggtattcc ctattgggga cgagtcacaa atcgttgggc
     2041 atttcagtcc cgttcattca cagagatcaa ttcattatgg atactcattc accaagtaca
     2101 accgatagcg aaactgaagc aaagaaactg aggagacatt tcaagttcac aaatgacgat
     2161 cttgaagaaa atcggcgtgg agtcctcagt aaaaagcaga tannnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nntttttgat agttagtcag tttttaaaaa tcaaaccata
     2341 taaactagtc agtattcgtg ggctcgctcg ccttgaaaaa ggacatggca atcgtttcag
     2401 ccatgtctat tatgatttac acatcaacaa tcaacagttc gatggagata gcactctaaa
     2461 caaggttatt gttcagggag ccgaatacat cgtatattat ttagaaagta atgcagaaat
     2521 aatgtctatt gaaaaagtgt caaacgagat gagtgtagct taagaatgaa attattctat
     2581 atttccatcc aaacgtccaa caaagcttgc tctgtctggg ggactgcccg gagggtatgc
     2641 gccaatctca aagatttttc tacgtcccag cgcaggcatc cctatcaggg acgagccaca
     2701 atcctttagg cggttaatct aaaccgcaat taaaatagct aacaatcgct gcaagtagta
     2761 acaaggatgc gcttgtaaca gcactggatg gtcaaatgaa catcaaattg ataatcaaaa
     2821 catttttcat cctactggtc agtctgataa tttcgagttg cggaacgaga ccgttattgt
     2881 tacaaactcc taccccaaca actctactta ccgcgatatc acaacctacc aaaataccaa
     2941 ccccttttgt cgccactttt gatgataacc tctgcgaaat aaaagggagc ttcatactgg
     3001 atggtgaact ttgctacatc aatgatgtat gtactaacgg aacagccgat agccgaaata
     3061 tagaaggttc acctagctac gcattaggtc tcttggggta ttacgcaaat ggtgattcgc
     3121 aatatctcag tatttggatt aagcatagtt tatctcccaa accaaacgaa atgcagactt
     3181 tgccaaacca ttttgagatc agcaatataa aaatttacac ttcaaacaac gtagtccttc
     3241 agagtgattt agaacctgtt cgtctcaaag tacatgtcat cccagctcaa ataacagagc
     3301 caggtgctga cgcttaccca ttactatctt gtgactatgt tgtggaagaa atacaattac
     3361 gataaactgc atataacaaa ctggtcaaca cagtgtatat caaagatact atctaattta
     3421 ctgactagat gagccaaggt gtgggcttcg caaaaaaata cagcaagtaa aggattggta
     3481 tgagtccgaa aaattcaac
//
LOCUS       DAOREF010000227         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16964_length_3498_cov_4.163811, whole genome shotgun sequence.
ACCESSION   DAOREF010000227 DAOREF010000000
VERSION     DAOREF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16964_length_3498_cov_4.163811"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..2266
                     /locus_tag="PKI33_05955"
     CDS             11..2266
                     /locus_tag="PKI33_05955"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNM36583.1"
                     /translation="MAKFTREDALEYHRLKGKPGKVAIVPTKPMDTQRDLSLAYSPGV
                     AEPVLEIEKNPADAYEYTSKGNLVAVISNGTAILGLGDRGALASKPVMEGKGVLFKKF
                     ADIDVFDIEVNSHDPDEIIKVVAAIAPTFGGINLEDIKAPECFYIEEELKKMLDIPVF
                     HDDQHGTAIISSAGLANALEIVGKKHEDIRIVISGAGASAISCAELAIRWGVKRENIM
                     LCDSKGVVYKGRKEGMNKYKERFLIETDARTLSDALRGADVFYGLSVANVMTPDMVKS
                     MAKDPIIFAMANPDPEIRPELAKEARKDVIIATGRSDYANQVNNVLGFPFIFRGALDV
                     RAKQINEDMKFAASKALAALAKEDVPDSVIRAYGGEPIKFGREYIIPTPLDPRVLLWE
                     APAVAEMAMKTGMARKTIDIDEYREQLAFRQGKGERIRYFFQNKARSSGGKKRVAFAE
                     GEEQKIIRAAYQIQEEGIATPVLIGRKDVIEKEIKSLSFDYKPEIVDPDNYFKLDAYA
                     KAFYELRQRKGLTVGDALKKVRDVNVLGSMMVKMGDADAFVSGLTYDYPEVIRPALQI
                     HHTAKGVARAAGVYIMIVEDKVFLFTDATVNIDPTADDLAEIAILAADYAKKIELDPH
                     VAFLSFSNFGSTPHPLSDKVRKAVNLVKSRRPDLRVDGEMQADTAVVPEIAEERYPFS
                     AVKDANVLVFPSLESANIAYKLLARLGNAKAIGPILLGLGAPVHVLQTGDDVNAIVQI
                     ASVAVMDAMGR"
     gene            2385..>3498
                     /locus_tag="PKI33_05960"
     CDS             2385..>3498
                     /locus_tag="PKI33_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36584.1"
                     /translation="MDNLENIARKITSVLFAQQSLASAGFIAAATLNSIVGKELSHNS
                     SWAGVPTAVYLLAGAFSAFMWGYVYDAIGRRYGLVSGLSTGVVGSSLTFYSIAIHSFP
                     LFLGGMVLMGIANSAVQLGRFAAAEVNKPEHRGQAISNVVIGGTVGSIIGPFVAGPAG
                     NLVGGWGIDELAGAYLVAAVLFVIGAIVVFTGLRPDPREIGKQVAEKYPETLIKHKHV
                     RGLLEIFRQPAALVALLSMVLGQMVMVLVMVITSLHMRGHNHNLSDISAVIASHTFGM
                     YAFSIFSGRLADKWGRGTVILIGSATLVIACIAATISPDVLPLGVALFLLGLGWNFCF
                     VGGSTLLADQLSPAERARTQGFNDLLVGLASALGSLE"
BASE COUNT          835 a          803 c          929 g          931 t
ORIGIN      
        1 tggaggctgt atggctaaat tcacacgtga agatgctctt gagtaccatc gtttgaaagg
       61 caagcctggc aaggtggcaa ttgtaccgac caagccaatg gatacccaac gcgacctaag
      121 tttggcgtat tctcccggtg tagcagagcc ggtgctagag attgagaaaa atcctgcgga
      181 tgcatatgag tacacatcca aggggaattt agtggcggta atctcgaatg gaacggcgat
      241 tctgggtctg ggtgaccgcg gagcgttggc aagtaagccc gtaatggaag ggaaaggcgt
      301 tctctttaag aagtttgcag atattgacgt ttttgatatt gaagttaact cgcatgaccc
      361 tgatgagatt atcaaagtgg ttgcggcgat tgccccaaca tttggcggta ttaaccttga
      421 agatattaaa gcccccgaat gtttttacat tgaagaagaa cttaagaaaa tgttggatat
      481 tcccgtcttt catgacgacc aacatggaac ggcaatcatt tcctcggcag gtttggcaaa
      541 tgccttggaa atcgttggta aaaaacatga agatattcgc attgtgattt ctggtgcagg
      601 cgcttctgcc atctcgtgtg ctgagttggc gattcgctgg ggtgtgaagc gtgagaacat
      661 catgttgtgc gacagtaagg gcgtggtgta caagggacgt aaggaaggca tgaataagta
      721 caaggaacgc ttccttattg aaaccgatgc gcggactctc tctgacgctt tgcgcggtgc
      781 agacgttttc tacggtttgt ctgtggcgaa cgtgatgaca cccgacatgg tgaagagtat
      841 ggcaaaagac ccaattattt ttgccatggc aaaccccgac ccggagattc gacctgaact
      901 ggcgaaagaa gctcgtaaag atgtcatcat agctacaggt cgttcagact atgccaatca
      961 ggtcaataac gtgttgggct tcccgttcat cttccgtggt gcgctggatg tgcgtgccaa
     1021 gcagatcaat gaagatatga aattcgccgc gtcgaaagcg ctggcagcgt tggcaaaaga
     1081 agatgtgcct gattcagtca tccgcgcgta tggaggcgag ccgatcaagt ttgggcgtga
     1141 gtacatcatc ccaactccgc ttgatccgcg tgtgctattg tgggaagctc ctgctgtagc
     1201 tgaaatggca atgaagaccg gcatggcacg caaaaccatt gacattgacg aatatcgtga
     1261 acagttggca ttccgtcaag gcaagggcga gcggattcgt tacttcttcc agaacaaggc
     1321 tcgttcttct ggtggtaaga aacgtgttgc atttgctgaa ggcgaagaac agaagatcat
     1381 ccgtgcggcg tatcagattc aggaagaagg cattgctact ccggtgttga tcgggcgaaa
     1441 agatgtgatc gagaaagaga ttaagtcact tagcttcgat tacaagcctg aaattgtaga
     1501 ccccgataat tactttaagc tggatgcgta tgctaaggcg ttctacgaat tgcgccaacg
     1561 caaagggttg acggttggag atgcacttaa gaaggtacgc gatgttaatg tattgggttc
     1621 aatgatggtg aagatgggcg atgcggatgc cttcgtctct ggcttaacct atgattatcc
     1681 agaggtgatt cgcccagcat tgcaaattca ccatacagca aaaggtgtgg cacgtgccgc
     1741 aggtgtgtac atcatgatcg tggaagataa agtattcctc ttcaccgatg ccaccgtgaa
     1801 cattgacccg accgcagacg atttggctga aatcgccatt cttgccgcag attacgccaa
     1861 gaagatcgag cttgacccgc atgtggcatt tctttcattc tcaaactttg gttcaacgcc
     1921 tcatccgctt tcagataagg ttcgcaaagc agtcaacctc gtcaagtctc gccgacctga
     1981 cttgcgcgtg gacggtgaaa tgcaagccga taccgctgtg gtgcctgaga tcgctgaaga
     2041 acgctatccg tttagtgcgg tgaaagatgc caatgtattg gttttcccat ctcttgaatc
     2101 agcaaacatc gcatacaaat tgctggctcg tctgggcaat gccaaggcaa tcggtcccat
     2161 cctcttgggc ttaggcgctc cagtgcatgt ccttcagacc ggcgacgatg ttaatgccat
     2221 tgtacagatc gcttccgtag ctgtgatgga tgcgatgggc agatagttct atagttggct
     2281 agtcaaatca tagttgagac cccagtaatc tttactgggg tcttttcttc tctccgcttc
     2341 aaaagtgtat aatccaaacc acctcaccca aaggaaatga tttcgtggat aatctcgaaa
     2401 atatcgcccg taagatcaca tccgtattat ttgctcaaca atcccttgct tctgcggggt
     2461 ttatcgcagc ggcaacctta aactccattg ttggcaaaga actaagtcat aactccagtt
     2521 gggcaggtgt gcccacagct gtttatttat tggctggagc gttttctgct tttatgtggg
     2581 gatatgttta cgatgccatt ggcagacgat atggactcgt cagcgggcta agcacaggtg
     2641 ttgtcggatc aagcctgact ttttactcga tcgccatcca ttcttttccc ttgttcctag
     2701 gcggaatggt tctcatgggc attgcaaact cagcagtgca acttggtcgc tttgctgccg
     2761 cagaagttaa caaacctgag catcgtgggc aagccatttc aaatgtcgtt atcggcggga
     2821 cggttggttc catcatcggt cccttcgtgg cgggacctgc cggaaacctc gtcggcggtt
     2881 ggggcatcga cgaattggct ggcgcatatc ttgtcgcagc ggttttgttc gtgatcggag
     2941 cgatcgttgt cttcacaggt ctgagaccag acccgcgcga gattggcaaa caggtggcag
     3001 aaaaatatcc agaaacgctg atcaagcata aacacgtacg aggcttgctt gaaattttcc
     3061 gtcagcctgc ggcattggtc gccttgctaa gtatggtgct gggtcaaatg gtgatggtgt
     3121 tggtgatggt catcacatcc ctgcacatgc gcggacataa ccataacctc tccgatattt
     3181 ctgccgtgat tgcctctcac acctttggta tgtatgcatt ttcaattttt tccgggcgcc
     3241 ttgccgataa atgggggcgt ggcacggtga ttttgattgg ttcggcaaca ctggtcattg
     3301 cctgtattgc ggctacgatc tcaccagatg ttcttccgct tggcgtggca ttgttcttgc
     3361 tcggtttggg ttggaacttc tgctttgtcg gtggttctac cttgctcgcc gaccaactct
     3421 ctcccgccga acgcgcccgc acccaaggct tcaatgacct tctcgttggt ctcgcatctg
     3481 ctcttggaag tttggaaa
//
LOCUS       DAOREF010000228         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16967_length_3498_cov_3.875399, whole genome shotgun sequence.
ACCESSION   DAOREF010000228 DAOREF010000000
VERSION     DAOREF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16967_length_3498_cov_3.875399"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1512)
                     /locus_tag="PKI33_05965"
     CDS             complement(28..1512)
                     /locus_tag="PKI33_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNM36585.1"
                     /translation="MKTKIITLESHDDLISVRDKLSWAKTPRILLVWPKYEKVTLRIL
                     DLKVLQRHADSLGAQLGLVTRRMNVRHDAESLGIPVFRTTTAAQKESWGEPIPRVQRT
                     PKSPQKGLRQLRDEVYIQEPAWRTSLFGRVVAFTAGVMAVLVLAGLFVPRAVVTVHSE
                     AQAVSLVIPVNASPFFDSVSLSGEIPAQVISVTVSNEQTVQVSSLVSVPKTKATGVVQ
                     FTNLGSDEIAIPAGTVVASNTLIRFATLNETRLPAGVDETVEVKVEALEAGAQGNVES
                     GAISVIEGTLGLSAKITNLEPTTGGSDEEVLGATEEDRATLRDSVIAGLTAIAEAQIH
                     SQIGANDLFISDTLTMKDIQNEEFTPPLGGEAPTLTYAVDAEFTAHYILEEDLKALAA
                     SSVMASIPQGFSPTGDMVFNPTETPFTDTTGVTRFALQATQSTLRDVNVMQVLDLIRG
                     RNVQQATLLVKDTLALQNEPQITMTPSWWKWLPLIPFNISVKVQ"
     gene            complement(1509..2258)
                     /locus_tag="PKI33_05970"
     CDS             complement(1509..2258)
                     /locus_tag="PKI33_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNM36586.1"
                     /translation="MTYNILNGGIGREEQILKVIQNAKPDVLVLQEVYSEDVLKFLSD
                     ALNMQYYFGKGNKRRKVACLSKLPVSSFKSVNPAFPIWRNIIDVEVETPVGQRVRVIG
                     VHPIAMLGVFFEIWRLWEAKYVIQYIQSLHEDYVVIAGDFNAIAPGEHVQTETMPKWL
                     KWMIYLQGNRVYHFSIKKYLSAGLTDCFRICNPNDKGFTLPPVRPNARLDYVLASEKM
                     KHYLKSATVLHMPDEVKVASDHLPLVVDFLT"
     gene            complement(2289..3431)
                     /locus_tag="PKI33_05975"
     CDS             complement(2289..3431)
                     /locus_tag="PKI33_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNM36587.1"
                     /translation="MIYLDYAATTPVDERVLAKMMPYFRDSFGNPSSIHRFGQKAEAA
                     VDEAREKVASVLGCRPDEVIFTSGGSEADNLALRGTMVANKASAKWLLTAKTEHPAVS
                     KTAIQLEKEYGFPLEWLDVDEHGVVTVESLSKAICNDTTMASVMYGNNEIGTINPIAD
                     LAKVAKSNNVLFHTDAVQAAAYLDVNVNKLGVDLMSLGGHKFYGPKGVGALYVRKGTK
                     LVPHLTGGSQESSLRAGTHNVPYIVGFAEALSLAAEEREARTAHVKPMRDHIIGTVLE
                     TISDSKLTGHPEQRLPNHASFVFKNVDGNLLLQMLDAAGFACSSGSACKTGNPEPSDV
                     VTALGHSHDWALGSLRITLGTGTTPEHVESFLQALPSLVEKTRSLS"
BASE COUNT          935 a          894 c          761 g          908 t
ORIGIN      
        1 caacggcaag gattctcata ctattgtcta ttgtactttc actgaaatat tgaaggggat
       61 caacggcagc cacttccacc aagaaggtgt catggtgatt tggggttcgt tttgcaacgc
      121 caacgtatct ttcaccaaca acgtcgcttg ttggacgttc cgtccgcgaa tgaggtctaa
      181 aacctgcatc acattcacat cgcgcaacgt ggattgcgtg gcttgcaatg caaaacgcgt
      241 tacacctgtt gtgtcagtga acggcgtttc ggtgggatta aaaaccatat cccccgtggg
      301 agaaaaccct tgcgggatgg acgccatcac agatgaagct gccaaggctt taaggtcttc
      361 ttccaaaatg taatgcgcgg tgaattctgc atcgactgca tatgtgagag ttggtgcttc
      421 cccacccaaa ggcggcgtaa actcttcgtt ttgaatatct ttcatcgtaa gcgtatcgct
      481 gataaacaaa tcattagccc caatttgcga atggatctgt gcttcggcaa tagcagtcaa
      541 gccagcgatt acagagtcac gtagcgttgc acggtcttct tcggtagcgc ccaatacttc
      601 ttcatcgctt ccgcccgtgg tgggttctag attcgtaatc ttggcagaaa gcccaagcgt
      661 tccttcaatt acactaatcg cgccagattc aacatttcct tgcgcccctg cttccaacgc
      721 ctccactttt acttccactg tttcatccac gccagcgggc aaacgagttt cgttcagtgt
      781 cgcaaatcga atcaacgtat tggacgccac taccgtccca gcagggatgg caatttcatc
      841 agagcctaaa ttcgtaaact gcaccacacc ggtcgctttt gtttttggca cagaaaccaa
      901 gctcgatact tggaccgtct gttcattgct aacggtgacg gaaatgactt gggcgggaat
      961 ctctccactt aaggagacgg aatcaaaaaa tgggctcgca ttgaccggaa tgacaagcga
     1021 gacggcttga gcttcggaat gtaccgtcac cacggctcgc gggacgaata agcctgccag
     1081 caccaaaaca gccatcaccc ccgctgtaaa cgccaccacc ctcccaaaga gcgacgtgcg
     1141 ccaagctggt tcctgaatat agacttcatc gcgcaattgg cgcaaaccct tttgtggcga
     1201 cttgggtgta cgctgtacac gtggaatggg ttcaccccat gattcttttt gtgccgccgt
     1261 tgtagttcta aaaacgggaa tccctaaaga ctcggcatcg tgacgtacat tcatacgacg
     1321 cgtcaccaac cccaactgcg ctccgagcga atccgcgtga cgttgtaaga ccttcaaatc
     1381 caatattcgc aaggtgacct tctcatactt gggccacacc agcaaaatac gcggcgtctt
     1441 cgcccacgat aatttatcgc ggacagagat gaggtcatcg tgagattcga gggtgatgat
     1501 cttggttttc aagttaaaaa atccactaca agcggtaaat ggtcagaagc taccttcact
     1561 tcatctggca tatggagaac tgttgcgctt tttagataat gcttcatttt ttcgctagcc
     1621 aacacgtaat ctaatctcgc gttagggcgt actggtggca aggtaaagcc tttatcatta
     1681 gggttacaga tgcggaaaca atccgttagc cctgcagata gatatttttt aatcgaaaaa
     1741 tgataaacac gatttccctg taaatatatc atccacttta accattttgg catggtttca
     1801 gtctgtacat gttcaccagg tgcaatcgca ttgaaatcac ctgcaatgac aacataatct
     1861 tcgtgaaggc tttgaatata ctgaataaca tacttagctt cccacagacg ccaaatttcg
     1921 aaaaaaacgc ccaacattgc aataggatga accccaatta ctctcaccct ttgccctact
     1981 ggagtttcga cttcaacatc aataatgttt cgccagattg ggaaggctgg atttacactc
     2041 ttgaaagatg aaactggcaa tttactaaga catgcaacct ttcggcgttt atttcctttg
     2101 ccaaaataat attgcatgtt gagtgcatct gataaaaatt tcaaaacatc ttcagaatag
     2161 acttcttgta aaactaatac atctggtttt gcattttgaa ttactttcag aatctgttct
     2221 tctcgaccaa tgccaccatt gagaatgtta tatgtcatta aacgtaacat aggggaattt
     2281 aagagtttct agcttaaact tcttgtcttc tctacaagcg aaggcaaagc ctgcaagaaa
     2341 gattcgacgt gttcaggggt tgtcccagtt ccgagcgtaa tgcgcagcga gcctaatgcc
     2401 caatcatggg aatgccccaa agcagtgacc acatcactgg gttcgggatt ccccgtcttg
     2461 caagccgagc cagaagaaca ggcaaaacca gcagcatcga gcatctgcaa aagtaaattg
     2521 ccatccacat ttttgaatac aaaagaagca tggttaggta aacgctgttc agggtgacca
     2581 gtgagttttg agtcagatat cgtctcaagc acggtaccga taatatgatc tcgcatgggt
     2641 tttacatggg cggttcgcgc ttcacgttct tccgcagcca gagacaaggc ttcggcaaac
     2701 ccaacgatat acggcacgtt atgcgtccct gcccgcaagc tagactcctg gcttccaccc
     2761 gtaagatgcg gcaccaactt cgtccctttg cggacgtaca atgcccccac ccctttcggt
     2821 ccgtagaatt tatgtccccc caacgacatc aagtccacac caagcttgtt cacatttaca
     2881 tcgagatatg ccgcggcttg gacagcatct gtatggaata agacattatt cgacttggca
     2941 acttttgcca agtctgcaat cgggttgatg gtgccaattt cattatttcc atacatcaca
     3001 gacgccatcg tcgtgtcatt gcaaatcgcc tttgaaagcg attcgaccgt cacaacacca
     3061 tgttcatcta catccaacca ctctaacgga aaaccatatt ccttttcaag ttgtatcgcc
     3121 gttttgctca cagcggggtg ttcggtcttg gcagttagca accacttggc cgaggcttta
     3181 ttcgccacca tggtaccgcg caaggcgagg ttatccgctt ctgagccgcc agaggtgaaa
     3241 atgacctcat cggggcggca tcctaaaacc gaggcaacct tctcgcgcgc ctcatctact
     3301 gcggcttccg ctttttgccc aaaacggtga attgaagagg ggtttccaaa cgaatcgcgg
     3361 aaatatggca tcattttagc gagcacacgc tcgtctacgg gtgtggtcgc ggcataatca
     3421 agataaatca tgactgggat tatagcaaag gttaatcata aaaagaccgg tctcaaaatg
     3481 ataaaatcat tccatgca
//
LOCUS       DAOREF010000229         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_16979_length_3497_cov_4.811447, whole genome shotgun sequence.
ACCESSION   DAOREF010000229 DAOREF010000000
VERSION     DAOREF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16979_length_3497_cov_4.811447"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..761)
                     /locus_tag="PKI33_05980"
     CDS             complement(135..761)
                     /locus_tag="PKI33_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36588.1"
                     /translation="MKTKLASWLCIFLFLTACSQSGDQPAVTQEPQQLPPTLTSTPEI
                     LPTVTPACIAQEPTQEDIDRSLAFTGQTFEQDDWEESYTVSEDRVSVSWFSNSLGAVA
                     FMEALIFPCGYEEPDLNNYFNDDNWGNVFANYESYELVSECRSDAAGLRLYQFDVESD
                     GVPYIVNYWVKNDTNTRVISFMIITPDDSFDLLDEYSYSIFPQLSNCS"
     gene            complement(859..1635)
                     /locus_tag="PKI33_05985"
     CDS             complement(859..1635)
                     /locus_tag="PKI33_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36589.1"
                     /translation="MQKDYLNYDQIATQYNQRYPNSQTWERGQALLELAKQVKAKTIL
                     EVGSGTGFWLNLLNQTGGKLFGLDYSMGMLQQSKGQPAPLSLSRGLGTQLPYRSNTFD
                     LLYCVDAIHHFGDHRAFIAEASRLLKPGGALAIIGHDPHEGPSHWYIYNYFDHVYDTD
                     LRRYPSGNRVLQWMEQEGLKNISDQTVESIHNIHVGDAVLKDPFLKHSATSQLALLNE
                     ADYQTGMERIQNALAAAKEKKERIVFRSEINVKMWLGFKK"
     gene            complement(1665..1868)
                     /locus_tag="PKI33_05990"
     CDS             complement(1665..1868)
                     /locus_tag="PKI33_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HNM36590.1"
                     /translation="MTNGQTRANIKPGITVLIVLKQDQRTGKLTKGIVKDILTNSPTH
                     PHGIKVRLTDGQVGRVKEIVAVS"
     gene            complement(1865..2179)
                     /locus_tag="PKI33_05995"
     CDS             complement(1865..2179)
                     /locus_tag="PKI33_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNM36591.1"
                     /translation="MSDQPARPIRFVVFHMPGPKWQQGVDYRQQAGVNEHVQHYFNLH
                     QQGKLQLGGPFLLPDMGGMMVTTKDVTYEEIDAFAAQDPAVQSGLLIYEIRPWMTAME
                     HE"
     gene            complement(2260..2760)
                     /locus_tag="PKI33_06000"
     CDS             complement(2260..2760)
                     /locus_tag="PKI33_06000"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM36592.1"
                     /translation="MNIQNIHLMFEYNYWANGRIFGAAKHISPEQLLAPAEFPYGDLR
                     ATLVHIIDAEYGWRGLFENDKFNDEIKPEEFANVQSIVERAMAEEKSMRAYLSKLTDA
                     DMESHKKYIADNGEPRDRILWHCLYHVVNHGSQHRSEAAAILTHFNASPGDLDFTVFL
                     NEVKPK"
     gene            complement(2784..3179)
                     /locus_tag="PKI33_06005"
     CDS             complement(2784..3179)
                     /locus_tag="PKI33_06005"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36593.1"
                     /translation="MASRIQALNHYRPQIEYGETAGWREVAEFLEASSTLSKADIIAA
                     LTGLQEAVIHFNLQGRGVKLEGLGTYLPNINYQGELDAAHRLDRRLKRGLNTGSFQGK
                     IRNKKNIGKTVEQIVSMWNAEHPDDPVLY"
BASE COUNT          823 a          781 c          924 g          969 t
ORIGIN      
        1 aatgcggcac ttgatagaaa cgattcggcg cggtgacggg tccgatcttg atgggctgaa
       61 agaggatatc gtagcgagag gacatgagat tttccagtga tgtatttacg attgtatgta
      121 tcgtctatcg actgttatga acagtttgat agttgtggaa agatcgagta actatattca
      181 tccagtaggt cgaaagaatc atctggtgta atgatcatga agctgatcac gcgtgtgttg
      241 gtatcgttct tgacccagta gttcacgatg tatggaacgc catcactttc cacatcgaat
      301 tgatacaatc gtaagccggc agcatcactg cgacactcgc taaccagttc ataactttca
      361 tagttcgcaa atacatttcc ccaattatcg tcattgaaat aattattcaa atcgggttct
      421 tcgtagccgc aggggaagat caaggcttcc atgaaggcaa cagcgcccag cgaattgcta
      481 aaccatgaaa cagatacacg gtcttctgat acggtgtagc tttcttccca atcgtcttgt
      541 tcgaaggttt gcccggtaaa tgcgagcgaa cggtcaatat cttcttgcgt tggctcttgg
      601 gcgatgcaag ccggtgtgac cgtaggaagg atttcaggtg tagatgtgag tgtgggggga
      661 agttgctgcg gctcttgcgt gacggcgggc tggtcgccag actgggaaca tgccgtcaga
      721 aaaagaaaga tacataacca cgatgcgagt tttgtcttca tagaatatcc cgtgcagttg
      781 gatttttctc gaagtatagc atgtggaaag aaaaacgccc ggctcgaaag ctgggcgttc
      841 ttggcgaaca ggctttgatt attttttgaa accgagccac atcttcacat tgatctctga
      901 ccggaagacg atgcgctctt tcttttcttt cgcagcggca agtgcattct gaatgcgttc
      961 catgccggtt tgataatctg cttcgttgag caaggcaagc tgagaggtgg cgctatgctt
     1021 gaggaagggg tctttcagca cggcatcgcc cacgtggatg ttgtgaatac tttccacggt
     1081 ttgatctgag atgttcttaa gaccttcctg ttccatccat tgcaaaacac gatttccaga
     1141 agggtaacga cgcaggtcag tatcgtagac atggtcgaag tagttataga tgtaccagtg
     1201 cgatggtcct tcatgcgggt catgcccgat gatggcgagc gctccgcccg gtttcagcaa
     1261 gcgagaagct tcggcaatga acgcgcgatg gtctccgaaa tgatggatgg cgtctacgca
     1321 gtagagcagg tcaaaggtat tggaacggta ggggagttga gtacccaagc cacggcttag
     1381 ggaaagaggc gcgggctgtc ctttggattg ttgcaacatg cccatcgagt aatccaaacc
     1441 gaataatttc ccgccggtct ggttcaacag attcaaccaa aagccggtgc cgctgcccac
     1501 ttccaaaatg gttttggctt ttacttgttt ggcaagctca agcaaggctt gcccacgctc
     1561 ccaggtttga gagttgggat agcgctgatt gtattgtgtt gcgatctggt cgtagttgag
     1621 atagtctttt tgcatggcat ccatttagat gccttaatga gccattaact tacagcgacg
     1681 atctctttaa ctctgccaac ttgcccatct gtaaggcgca cttttatgcc atgcggatgt
     1741 gtaggcgaat ttgtgagaat gtctttgaca atgcctttgg tcagtttgcc agtgcgttga
     1801 tcttgtttga gaacgatcag caccgtgata ccgggtttaa tatttgcgcg ggtttgtccg
     1861 ttggtcattc atgctccatg gcggtcatcc acgggcggat ctcgtagatg agcaagcctg
     1921 attgtacagc cgggtcttgg gcggcgaagg cgtcgatctc ttcgtaggta acgtctttgg
     1981 tggtcaccat catgccgccc atatcaggca aaagaaaggg tccgccgagt tgtagtttgc
     2041 cttgctggtg aaggttgaaa taatgttgaa catgctcgtt aacaccagct tgctggcgat
     2101 agtctacacc ttgttgccat ttgggtccgg gcatgtggaa gacgacaaaa cgaatgggac
     2161 gcgcgggttg atcagacata agaagctcct atatgtattg gatgataaac ccgcatcata
     2221 ccgacccttt gccgggtacg caccattctg ccgtagtgtt tatttgggct taacctcatt
     2281 cagaaagacc gtaaagtcca aatctccggg agaggcgttg aagtgggtga gaatggcagc
     2341 cgcttcactg cgatgttgcg agccatggtt gaccacatga tacaagcaat gccaaaggat
     2401 gcggtcgcgg ggttcgccat tatcggcgat atatttttta tggctttcca tgtctgcatc
     2461 ggtaagtttg ctgaggtaag ctcgcatgga tttttcttct gccatggcac gttccacaat
     2521 agactggaca ttggcgaact cttcgggctt gatctcatca ttgaatttgt cattctcaaa
     2581 caaaccgcgc cagccatact cggcgtcgat gatgtggacg agggtggcgc gtaaatctcc
     2641 atacggaaat tctgccgggg caagcagttg ttctggggag atatgtttcg cagcgccgaa
     2701 gatgcgcccg ttcgcccagt agttgtattc gaacataagg tggatgtttt gaatgttcat
     2761 tggcttctcc agtaggtact ttttcagtat agcacggggt catcggggtg ctctgcattc
     2821 cacattgaaa caatctgttc tactgttttc ccaatattct tcttgttgcg gattttccct
     2881 tgaaaagaac ccgtattcag tccacgcttt aatcgtctat ccagacgatg cgccgcatca
     2941 agctcgccct gatagttgat attcggtaaa taagtgccca gcccttcgag cttaaccccg
     3001 cgcccttgaa gattgaagtg gatgacagcc tcttgtaacc cggtcagggc ggcgataatg
     3061 tctgccttgc tcagcgtaga gctggcttcc aaaaactcgg caacttcccg ccagccagcg
     3121 gtctcgccat actcgatttg cggacggtag tggttgagtg cttgaattcg agacgccatg
     3181 caattcctcc tgtcttgtta atgaacaaga cttgtcttaa tcctaatatc ctgcaaggat
     3241 attgattatg ttgttgcagg atattataaa tatgctggca gggtattgtc aattaaacag
     3301 tagaggttgg ggaaacggac agtatatttt ggaaaaccag acctccgagg tttttaaaac
     3361 ctcggaggtc tgggatgcaa tgtgtcttgt cattctgagg gcgcgtgttc tccgcgaccg
     3421 aagaatctct agggttatgg tagagatcct tcgcctatcg gctcaggatg acaccttatt
     3481 tgtttcatgc gctgata
//
LOCUS       DAOREF010000230         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17016_length_3492_cov_4.483852, whole genome shotgun sequence.
ACCESSION   DAOREF010000230 DAOREF010000000
VERSION     DAOREF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17016_length_3492_cov_4.483852"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..2086)
                     /gene="htpG"
                     /locus_tag="PKI33_06010"
     CDS             complement(245..2086)
                     /gene="htpG"
                     /locus_tag="PKI33_06010"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNM36594.1"
                     /translation="MTDAQQFTFKAETKQLLQILIHSLYKDREVFLRELLSNASDAIN
                     RLRFEMLTNQNVLDADAELKIHVKVDKDNRTITVQDTGIGMTQDELVDHLGTIAQSSA
                     RKFLEAAKEKNQDASGLIGQFGVGFYSVFMVAEWVRVTSRSFRPDAQAVTWYATGDDT
                     YQVSPAEMTERGTRIEIKLKEDAAEFAEEYRLKNIIHKHSDYIGFPIYLGDEEKPVNK
                     QTSLWRTARNEITAEQYKDFYRQTTLDFEDPLMHVHMVTDAPVQLYALLFFPAKSERN
                     MFSLRKEDGLKLYSRNILIDEYNKDLLPEYLRFVQGVVDSEDLPLNVSRETVQSSGLM
                     PKLKKVLTNQVIKELETLAKKDAEKYASFWSEFGVYLKQGIAASPADTDTITPLLRFK
                     TNTNPETWSSLDEYVSRMKDGQKVIYYIVGEDPKAVLRSPHLDAYKANGTEVLLLTEP
                     MDSFMLMGLRKFKDYELKSVSGADADGAEQPKAESEAEKIPEGDFNALIEKFKQVLGE
                     RVTDVRASNRLVSSVARLVDADANSNPELQRVYKYLGKEYEVPKKVLELNPSHEMLKS
                     LLNTEGELQTIIIEQVFDSALLVEGLHPDPSSMAERVQRIMEAALKK"
     gene            2352..3257
                     /locus_tag="PKI33_06015"
     CDS             2352..3257
                     /locus_tag="PKI33_06015"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNM36595.1"
                     /translation="MDRKEFGILVSHLRQDLNLTQFELAENAEVDIAVISQIERGVKK
                     HLDSELLFKLANSFHLTTIERREFFLAACGLGETRIIRQPSAALATDTINAEKVLGKM
                     VKIVEKLSIPAFIMDVYSDVIAINYIAAAFFQVTPDVLDVASKVPGGYSTVRMMFGKD
                     LHLRSKVLENWDHYAVGSMRFVRENSLRYRAKPYFQYLMKSFQDRNEFPLFERYWRMV
                     SSFEQDREANVDVFSYTHADYGPLNYATATTVSITPHGELFLNQYLPLDAHTRNLFSA
                     LEQQTGQGVVTELASWPEKKIPMNT"
BASE COUNT          874 a          920 c          786 g          912 t
ORIGIN      
        1 gtaattcaaa atattttggt tttataaaaa tttatttggt gaaaagcggg tgttagcgag
       61 taaaccactg cctatacgca aaaaaggagg tcatctagca aagatgacct ccttccctac
      121 ttttcattgt gtcatgctga gccgcgatag cggcgaagca tctctacaat tatcctagag
      181 accctcaccg cactgcatcc gtactgcggt gcatgtgtcg ctatcgctca gggtgacaat
      241 cctgctattt cttcaacgcc gcttccataa tcctttgcac acgctccgcc atgctcgaag
      301 ggtcggggtg caagccctcc accagcaagg cgctatcaaa gacctgctca atgataatgg
      361 tctgcaactc gccttcggtg tttagcaaac tcttcaacat ctcgtgcgaa gggttcaact
      421 caagtacctt ctttggcact tcatattcct tgccgagata tttatacacg cgctgaagtt
      481 ccgggttgga gttcgcgtcc gcatccacca agcgcgccac tgacgacacc aagcgattgc
      541 tggcacgcac atcggtgact cgctcgccga ggacttgctt gaatttctcg atgagggcat
      601 tgaagtcacc ttcggggatc ttctccgcct cagactccgc cttgggctgc tctgccccat
      661 cagcatccgc tccgctgacg cttttcagct catagtcctt gaacttgcgt aagcccatca
      721 gcatgaacga gtccatgggc tcggtcagca acagcacctc tgtgccattc gctttgtacg
      781 catccaagtg cggacttctc agcaccgctt tggggtcttc gcctacgatg tagtaaataa
      841 ccttctgtcc gtctttcata cgtgagacgt attcatccaa cgaagaccag gtttcggggt
      901 tagtattggt cttgaagcgc aataacggcg tgatggtgtc tgtatctgcc gggctggcag
      961 caataccttg tttgaggtac accccaaact cactccagaa cgaggcatat ttttcggcat
     1021 ctttctttgc gagtgtttcc aactctttga tgacctgatt ggtcagcacc ttcttgagct
     1081 tcggcatcag tccgctcgat tgcacggtct cacgcgagac gttgagcggc aaatcttccg
     1141 agtccaccac accttgcacg aaacgcagat attcgggcaa caggtctttg ttatattcgt
     1201 cgatcaggat attgcgtgag tacaacttca agccgtcttc tttgcggagc gagaacatat
     1261 tgcgttctga ctttgccggg aagaacagca aggcatacaa ctgcaccggc gcatccgtca
     1321 ccatatggac gtgcatcagc gggtcttcaa aatcaagcgt ggtctgacga taaaagtcct
     1381 tgtactgttc ggcagtaatt tcattgcgag ccgtacgcca caacgaggtc tgcttgttga
     1441 ccggcttctc ttcgtcgccc aggtaaatgg gaaagccgat gtaatctgaa tgtttgtgga
     1501 taatattctt caggcggtat tcttcggcaa attcggcggc atcctctttc agtttaattt
     1561 cgatgcgtgt accgcgttcg gtcatctccg ccgggctgac ctgataagta tcgtcacccg
     1621 tggcgtacca agtcacggct tgagcatcgg ggcggaagga acgcgacgtg acccgcaccc
     1681 actctgccac cataaatacg gagtaaaaac ccacgccgaa ctgaccgatc aaaccggaag
     1741 cgtcttgatt cttttccttc gccgcctcca aaaacttgcg cgcgctagat tgggcgatcg
     1801 tcccgaggtg atctaccagc tcatcctggg tcataccaat gcctgtatcc tgaaccgtga
     1861 tagtgcgatt gtctttatcc accttcacgt ggatcttcaa ttcggcgtca gcatccaaca
     1921 cattttggtt ggtcagcatc tcaaagcgca agcggttgat cgcatcagac gcattcgaga
     1981 gcagttcacg caaaaaaact tcccggtctt tatacaggga atggataagg atttgcagca
     2041 gttgtttggt ctctgctttg aaagtaaatt gttgggcatc tgtcatggtt gcctcctgaa
     2101 agatgaagga ttttccctat ttatagccgc aaatcgtaaa aatttcgtaa gagacttact
     2161 taaaataaaa cctcagcctt aaggctgagg tcccaaatgt agttcccctc ccccactaca
     2221 tgaggcaggg ttgcaagaac tggaaagaaa caactcaaat attgtacgat tttgggttga
     2281 aaagtccagc aaaagagttt aaacttgact cgaaaaaatt tttgagtcga atttatttgg
     2341 taggagccgt catggaccgc aaagagtttg gaatcctcgt ttctcatctt cggcaagacc
     2401 tcaacttaac gcagtttgaa ctggcagaaa atgcagaagt ggatatcgca gtcatcagtc
     2461 aaattgaacg aggcgtgaaa aaacatcttg attcagaatt gcttttcaaa ctcgccaatt
     2521 cgtttcacct tactaccatt gaacgccgtg agttcttcct tgctgcttgc gggctcggcg
     2581 aaactcgcat tatccgccaa ccctctgccg cgctcgcaac cgatacgatc aatgccgaaa
     2641 aagtcctcgg caaaatggtc aaaattgttg aaaagctaag catccccgca tttatcatgg
     2701 atgtctattc agatgtgatt gccatcaact atatcgccgc tgctttcttt caagttacac
     2761 ctgacgttct ggatgtcgca tcgaaagtcc ccggtgggta tagcaccgtc cgcatgatgt
     2821 ttggaaaaga cctgcacctc cgttccaaag tgcttgaaaa ctgggaccat tacgccgtcg
     2881 gctcgatgcg cttcgtccgt gagaacagct tgcgataccg tgccaaacct tattttcaat
     2941 atctcatgaa gtcattccaa gaccgaaatg aatttccgct cttcgaacgc tattggcgca
     3001 tggtctcgtc ctttgaacaa gaccgtgaag ccaatgtaga tgtcttcagt tacacccatg
     3061 ctgattacgg tccgctcaac tatgcaaccg ctactaccgt ctcgatcacc ccacatggcg
     3121 aattattcct caaccaatat ctgccactcg atgcccacac ccgtaatctt ttttctgcac
     3181 ttgagcaaca aacagggcaa ggtgttgtca cagagcttgc ctcatggccc gaaaagaaaa
     3241 tccctatgaa cacctgatat tcgtcatatt gaaggcagga atgtcctcct tataatcatt
     3301 aatagcgcca cgcttatgta acaagaagcg taagcaatat gaataaggag aatgtcatga
     3361 cgaaactcga tagtatggtt cagaacttcc tcgcccaaaa acggatcgcc gttgtaggcg
     3421 tttcagacaa gcgcgaaacc ggatgcaatg ccgcctacca aaaattcaag gatgcaggct
     3481 acgaagtctt cg
//
LOCUS       DAOREF010000231         3481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17109_length_3481_cov_4.359311, whole genome shotgun sequence.
ACCESSION   DAOREF010000231 DAOREF010000000
VERSION     DAOREF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17109_length_3481_cov_4.359311"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1472)
                     /locus_tag="PKI33_06020"
     CDS             complement(186..1472)
                     /locus_tag="PKI33_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36596.1"
                     /translation="MSSSRLNYGKTFLLGFGFFGVSVIWGVYNAFVPIFLSEKFNLSP
                     VLIGFFMTLDNIAALFIQPPVGAWSDKLRTPIGRRIPFILIGAPITALAFGLIPIAAV
                     LPLFVACTSTLLLSAALWRTPVVALMPDITPSEFRSQANGIINFMGGIGTIIALQTGG
                     ILYKMSPSFPFWLGSGLVLIAALIVYLFIEEPKEYEAAEEQPGMFASLKEVVSDPDKS
                     GLRILLAIFFWFIGFSAVETFFTLYAKNHLGLNEGDGATLLSVLPLFFVLAAIPSGYV
                     AGKLGRKTTIATGLIIITIMLVLLYITPAEALLTGIAPLPLVGIPLVEGGARMLTLAG
                     VLLIIGGIGWAFININSLPMIVDLTSAARIGTFTGLYYLFSTLSAIVGPNLNGWAVQL
                     TNGNYNIIMLIAPFFMLIALGLMMGVKKGEAVSSAG"
     gene            complement(1532..>2131)
                     /gene="trmD"
                     /locus_tag="PKI33_06025"
     CDS             complement(1532..>2131)
                     /gene="trmD"
                     /locus_tag="PKI33_06025"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNM36597.1"
                     /translation="YGGGGGMVMKPEPVFEAIETVLGLASPLSTPEPESNIPIILLTP
                     QGRVFNQSIAKELSQHKKIALLCGRYEGIDERIRENLVTDQISIGDYVLTGGELPALI
                     LIDAVSRLLPNVLGDPTGAEDDSHAMGLLEYPHYTRPPEFRGNKVPDVLLSGDHKKID
                     KWRREQALLRTLQKRPDMLETAELTKEDKKFLEKLKNQS"
     assembly_gap    2132..2231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2266..>3481
                     /locus_tag="PKI33_06030"
     CDS             2266..>3481
                     /locus_tag="PKI33_06030"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNM36598.1"
                     /translation="MIGITGCNLPLPTCATDSLQEVTLLSPAKMGVVDTLEPTLEWSY
                     PSSECTPEGYAITMWEGPVMTENIGGGTGNPNTQWVPGSPLEPGKQYRWTVAPINGTT
                     LGPVSETFVFFTGPVCTDASLVPPTMLEPADGASFNATTDLLWWDYTAGCTPTGYQLD
                     VATDSSFASGLYPSGIIDIPLTRAVLGDPPLVECQTYYWRVAARNGDTIGSYSPTRSF
                     VADTTGTCGGSGGGGGGTPPDSASISGIVWHDICAIPDGPLPDPLSAGCVEDGAGSAR
                     ANGIREEGEPGIAGVQVNLYYGDCDFAGASDASVLTDADGRYIFDGLTSFGNFCVAVN
                     PGTSPNDAILLPGEWTYPDLITPDGVAYQTFGGIESGVHVDIIDFGWDYQFLPAFNDP
                     TPTPVAGNLTPNI"
BASE COUNT          873 a          776 c          890 g          842 t
ORIGIN      
        1 cactttcttg cgctgcttgt aaccccgccc atgcattttg attcatgccg tggtcttcaa
       61 tgccaagagt atctgtcacc aatgcggcgc agaagacatc ctcatcaaaa caatctggcg
      121 atgataggca ggaacttaaa gcccatgcca tcatcgccag aaaaaaatac ttttggaatc
      181 gcaagttaac ccgcgcttga aacagcctcg cccttcttta cacccatcat caaaccgagt
      241 gctatcaaca taaagaatgg agcgatcaac atgatgatgt tgtaattgcc atttgtgagc
      301 tgaaccgccc aaccattcaa gttgggaccc acaatggcag ataaggtgga gaagaggtag
      361 tagagacccg tgaatgtacc aatgcgtgct gcactggtta aatccacgat cataggcagg
      421 gagttgatat tgatgaatgc ccaaccgatg ccgccgatga tgagcaaaac gccagccaat
      481 gtgagcatac gtgcgccgcc ttccacgagc ggaatgccga ccaatggcaa aggtgcgata
      541 ccggtcaata acgcttcagc aggagtgata tagagcagga ccaacatgat ggtgatgatg
      601 atcaaaccgg tagcaatggt ggttttgcgt ccgagttttc cagccacata gcctgaagga
      661 atggctgcca gcacaaagaa caacggcaga acggagagca aggtcgcgcc gtcaccttcg
      721 ttcaagccga gatgattctt ggcatagagt gtgaagaagg tttcaaccgc agagaaacca
      781 atgaaccaga aaaagatggc aagcaaaatg cgcaacccgc ttttatcggg gtcgctgact
      841 acttctttga gactggcaaa cataccgggt tgttcttcgg cggcttcgta ttctttgggc
      901 tcttcaatga aaagatatac gatcaacgca gctataagca cgagcccaga gcctaaccag
      961 aatgggaagc tgggactcat tttataaagt atgccgccgg tttgcagggc gatgatggtg
     1021 ccaattccgc ccatgaagtt gataatgcca ttggcttgcg agcgaaattc agagggtgtg
     1081 atatcaggca ttagcgctac tacaggggtg cgccataaag cagcactgag caagagcgtg
     1141 ctggtacatg ccacaaatag cggaagcact gctgcaatag gaatcaaacc aaatgccagg
     1201 gcggtgatgg gtgcgccgat caaaataaat ggaatgcggc gaccaatggg ggtgcgcaat
     1261 ttatcagacc atgctccaac aggcggctga atgaacaatg ccgcaatgtt atcaagggtc
     1321 atgaagaaac cgattaacac aggggagagg ttgaattttt cagagagaaa aatgggaacg
     1381 aaggcgttat acacacccca gatcacactc acaccaaaaa aaccgaagcc gagcaggaag
     1441 gtcttgccgt agttcaatct tgacgatgac atcatagcct ccaggttggt ttacaggttg
     1501 gtaggtttaa atgcttaagt ctgccaacgt tttaactttg attctttagc ttctcaagaa
     1561 actttttatc ttcttttgtg agttctgcag tttcaagcat atcggggcgc ttttgaagtg
     1621 tgcgtaaaag tgcttgttct ctgcgccatt tgtcaatttt tttgtggtca ccagagagaa
     1681 gaacatcggg cactttgttg ccgcgaaact caggcggacg ggtgtaatgg ggatactcca
     1741 acaagcccat ggcgtgcgag tcatcttcgg cgccggtagg gtcgccaagt acattgggca
     1801 ggagacgaga aaccgcatca attaggatga gcgcgggcag ttcgccacca gtgagcacat
     1861 agtcgccaat ggagatttgg tcggtcacca agttctcgcg gatgcgttcg tcaatgcctt
     1921 cataacgtcc gcatagcagg gcgatctttt tgtgttgtga taattctttg gcaatggatt
     1981 ggttgaagac gcgtccttgc ggcgtaagca aaatgattgg gatgtttgat tcaggctccg
     2041 gcgtggaaag aggcgaagca agtcccagaa cggtttcaat ggcttcgaaa actggctctg
     2101 gcttcatcac cattccgccg ccgccgccgt annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn naaattttac ttttgtgttc ctgctttttg ttttgatgat cggaataacg
     2281 gggtgtaatt taccgctgcc tacctgcgct acagatagtt tacaagaagt cactctgctt
     2341 agccctgcaa agatgggtgt tgttgatacg ttagaaccca cgcttgaatg gagttatcca
     2401 tcttctgagt gtacgccaga agggtatgct attacgatgt gggaaggacc cgtgatgact
     2461 gagaacattg gtggtgggac gggtaacccc aacacccagt gggtaccagg ttcacccctt
     2521 gaacccggca aacaataccg ctggacggtt gcgcccatca acgggacgac gctaggacca
     2581 gtttcagaaa cgtttgtttt ctttacagga ccagtgtgta ccgatgcgtc tcttgttccc
     2641 cccactatgc ttgagccagc cgatggagca agtttcaatg caacgacaga cctattatgg
     2701 tgggattaca cggcagggtg tacgcctaca gggtatcaac tggatgtcgc aacggattca
     2761 tcttttgcaa gcggactata cccatctggg attattgata ttccactaac aagagctgtg
     2821 ttgggcgatc ctccattggt ggagtgtcaa acctattact ggcgtgttgc cgcacgaaac
     2881 ggcgatacta ttggctcgta ttcgccaacg cgtagttttg tcgctgatac tactggtacg
     2941 tgcggtggta gtggcggtgg cggcggtgga actccacctg actcagcttc tatcagcgga
     3001 attgtttggc atgatatttg tgccatacct gatggaccgc ttcctgaccc gctttcagct
     3061 gggtgtgtag aagatggagc tggttctgct cgtgcaaatg gaatccgcga agaaggcgaa
     3121 cctggtattg caggggtaca agttaattta tattatggcg attgcgattt tgctggagca
     3181 agtgacgcaa gcgttctaac agatgccgat gggcgatata tatttgacgg tctcacttca
     3241 tttgggaatt tttgtgttgc ggttaacccg ggcacatcgc ctaacgatgc catcttactc
     3301 cctggcgaat ggacttaccc tgatctgatt acgccggacg gtgtggcata tcaaaccttt
     3361 ggtggcattg agtctggtgt gcatgtggat attattgatt ttggttggga ttatcaattc
     3421 ctaccagctt ttaatgaccc tacacctact ccagttgcag gaaatctaac gccaaacatt
     3481 c
//
LOCUS       DAOREF010000232         3478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17132_length_3478_cov_4.195735, whole genome shotgun sequence.
ACCESSION   DAOREF010000232 DAOREF010000000
VERSION     DAOREF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17132_length_3478_cov_4.195735"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKI33_06035"
     CDS             <1..558
                     /locus_tag="PKI33_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNM36599.1"
                     /translation="GQSGWSSDALINGDQGLESQLTRAVSELQDATAQGKAALALVWI
                     GSNDLWYLYEYGGDIDDAGDTSDAQRFEANIDTILSQLRGTGAQVVIALLDDQSKRPV
                     ALKGEAFVSITPDEMNRMSAHVDVYNQIIRDKAAQYGALTVDFYSTDIFTNPATLYDD
                     GNHPNQAGYDIIAQKWFDAISPLLK"
     gene            complement(599..1609)
                     /locus_tag="PKI33_06040"
     CDS             complement(599..1609)
                     /locus_tag="PKI33_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM36600.1"
                     /translation="MKKIFPFLFLALLLSSCISVEVKEADTPVPSNFVTATLAPTKAG
                     YVPPTFTETPAITQTPTFAVTIDPNCQDTAVLVRDVTVPDGTQMKPGEKFTKTWEFIN
                     SGDCPWYGYTIKFAAGDQMNAPLSAPIPDTLIKKSVQVSVDLTAPTTNGSFTGYFTLN
                     NPTGKDVPIGIEKTFWVKITVGSGSPAPQATISSSTQSSLGSVGANCNYSQNAGYVNQ
                     IASLINQERANAGLPALTINSLLTNAAQIHAADMACNKNISHTGSDGSSAYARILASG
                     YAPSYAEEIIYGGGGPQAAMTWWMNDQIHRDAILSAKSSEMGVGYANYSGDYIVVDFG
                     SP"
     gene            complement(1651..>3478)
                     /locus_tag="PKI33_06045"
     CDS             complement(1651..>3478)
                     /locus_tag="PKI33_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36601.1"
                     /translation="QIYAQAFLWRKGEDVPYAKIYECPHCKDSGERIATDSDKEIALQ
                     IAGTDTLHRTRLSERVVSLNDEDRVYAEEAIEHYLPRPLYVLGTILNRLDSLDLNESR
                     RRALTALLLLAFDAGNTLWGHPAERPRPKLLNTPSQFRENNLWTMLERGIRLFAESGS
                     PVVVEAWPKKIPETGGICIYEGRLKDLAHEVKREIPIAAVVASIPRPNQAFWTLSALW
                     AGWLWGSDAVEPYKVALRRRRYDWAWSATALFAMFSHLDELLADGVPFFGLMPEPEAP
                     FMTSALTAASAAGFKLESVALRTEHDPVQVLWKSGKKSASAEIEVETARNAMRSYIAA
                     RGEPAGYLHLHTAGLIALAEVNALKQANDEFDFAMRKTQSVMEQALKDSHEFVHYSTG
                     EGVDTGVWSLASGHAESLSDKIEVGIVNYLQKNPQTIFLEVEEELNKQFTGLMTPSKG
                     LIYAILNSYAEKDGGIWNLRNEDYASARRDEMKTVFALLEEIGKRLEYKTKIEEKTVT
                     WFESGKAVRKFYVMASALIHRALEQIDEQTVIVIPGGRAALAAYKQERDPSLKERLKK
                     HKLAKYRLLRSVLDLPILTRETFEELIASDPVEKAENQLMMF"
BASE COUNT          877 a          895 c          794 g          912 t
ORIGIN      
        1 ggtcaatccg gctggagttc cgatgcgctt atcaacggcg atcagggatt ggaaagtcag
       61 cttacgcgtg cggtgagtga attgcaagat gcaactgccc aaggcaaagc ggctctggca
      121 ttggtatgga tcggcagcaa cgacctttgg tatttgtacg aatacggtgg tgacattgac
      181 gatgccggcg acacttcgga tgcgcaacgt ttcgaagcga atatcgatac catccttagc
      241 caactgcgtg gaaccggcgc ccaagtggtt atcgccttgt tagacgacca atccaaacgc
      301 ccggtggcac tgaaaggcga agctttcgtt tccatcacgc cggatgaaat gaaccgcatg
      361 tctgcccatg tggatgtcta caaccagatc attcgcgata aagctgccca atatggcgca
      421 ctgaccgtgg acttctactc gaccgatatt tttaccaacc ctgctacgct ctacgacgat
      481 ggcaatcatc ccaaccaagc tgggtacgat atcatcgccc aaaaatggtt tgacgccata
      541 tcgcctctgc tgaaatagag aaacaaaaag tctcttgcgt ttgcaagaga ctttttgttt
      601 atgggctgcc gaaatctacc acaatgtaat cgcctgaata attggcatac ccaacgccca
      661 tttctgacga ttttgcacta agaattgcat cgcgatggat ttgatcgttc atccaccagg
      721 tcatcgccgc ctgcggacca cctccgccgt agatgatttc ttccgcatac gatggtgcgt
      781 atcctgaggc aagaattcga gcatatgctg aagagccatc tgaacccgta tggctaatat
      841 ttttattaca tgccatatct gcggcgtgaa tctgtgctgc attcgtcagc aaggaattaa
      901 tcgtcaaagc aggtaaaccg gcatttgccc gttcttgatt gatcaacgat gcgatttgat
      961 taacataacc ggcattttga ctgtaattgc agtttgctcc tacactacct aaagacgatt
     1021 gcgtgctact agaaatcgtc gcttgtggag ctgggcttcc actgccaacg gtgatcttca
     1081 cccaaaacgt tttttcaata ccaattggca cgtctttacc cgttgggttg ttgagcgtga
     1141 agtaacctgt aaaggatccg ttcgtggttg gggcggtcaa atccactgaa acttggacag
     1201 atttcttgat aagcgtatcc ggtatcggcg cggaaagagg cgcattcatt tgatcccctg
     1261 ccgcaaattt aatggtgtat ccataccacg ggcaatcgcc gctgttgatg aattcccaag
     1321 ttttggtgaa tttttcaccg ggcttcatct gcgttccatc agggacagtc acatcgcgga
     1381 cgagcacggc ggtatcctga cagttcgggt cgatggtgac tgcaaaggta ggcgtttgtg
     1441 tgatcgccgg agtctcggtg aaagttggcg gaacataccc cgccttcgta ggagctaagg
     1501 ttgccgttac aaaattagaa ggcacggggg tatctgcttc tttgacctcg accgaaatac
     1561 aggaagataa aagaagtgcg agaaaaagaa aagggaatat ttttttcatt gagttttacc
     1621 aaagctattt tcgttcaccg atgaagtgag ttagaacatc atcaattgat tttcggcttt
     1681 ttccaccggg tcactggcga ttagctcttc aaatgtttcg cgagtcagaa taggcaggtc
     1741 taacacactg cgcaatagac gatattttgc caatttatgt ttcttcaatc gttccttgag
     1801 cgaaggatcg cgttcttgtt tataggctgc caacgctgcc cgcccgccag ggataacgat
     1861 gacggtctgt tcatcaattt gttccaacgc acggtggatc aatgccgaag ccattacata
     1921 aaatttacga acagcttttc cactttcaaa ccaagtgact gttttttctt caattttagt
     1981 tttgtattcc aggcgtttcc caatttcttc caacaatgcg aaaactgttt tcatttcatc
     2041 acggcgagct gacgcataat cttcattgcg caaattccaa attccgccgt ctttctctgc
     2101 ataggaattc aggatggcat agatcaaacc tttggatgga gtcatcaagc ctgtgaactg
     2161 cttattcaat tcttcttcga cttccaaaaa gatcgtttgc ggattttttt gcagatagtt
     2221 cacaatgcca acttcgatct tgtctgaaag cgattcagca tgtccgcttg ccaggctcca
     2281 tacaccggta tcaacgcctt cgcccgtaga gtaatgaaca aattcatggc tatctttcag
     2341 ggcttgttcc atcacacttt gagtcttgcg catggcaaag tcaaattcat cattggcttg
     2401 cttcaatgca ttgacctccg ccaacgcgat cagccctgcg gtatgcaaat gcaaataccc
     2461 cgccggttca ccacgtgctg ctatatagct acgcattgca ttacgtgccg tttctacttc
     2521 tatttcagca gatgctgact ttttgccgct tttccataac acttgcacgg ggtcatgttc
     2581 agtgcgcaat gcgacacttt ccaatttgaa ccccgccgcc gatgccgcgg ttaaagccga
     2641 agtcataaaa ggtgcttcgg gctcaggcat caaaccaaag aacggcacac catctgcaag
     2701 caattcatcc aagtgactga acatcgcgaa caaagctgtg gcactccatg cccagtcata
     2761 tcgcctgcgt cgcaacgcta ctttatatgg ctccaccgcg tcactgcccc acaaccagcc
     2821 tgcccacaat gccgaaagag tccaaaacgc ctgattgggt ctcggaatag aagccaccac
     2881 cgccgcaata ggaatctcac gtttgacttc atgggcaagg tctttcaagc gaccttcata
     2941 gatgcagatg ccgcccgtct ctggaatttt cttgggccac gcttcaacca caacgggtga
     3001 gcctgattca gcaaacagac ggatgccacg ctcgagcatg gtccacaaat tgttctcgcg
     3061 gaattgactt ggtgtgttca gcaacttcgg gcgcggacgt tccgccggat gcccccaaag
     3121 cgtattgcca gcatcgaaag ccagtaacaa caaggcggtc aacgcacggc gacgtgactc
     3181 gttcaaatcc aaactatcca accgattcaa aatggtaccc aacacataca acggacgcgg
     3241 caaataatgc tcaatggctt cttcagcata gacacgatct tcatcattca gtgaaaccac
     3301 ccgttcagat aaacgtgtgc gatgtagagt atccgtccct gcaatttgca acgcgatttc
     3361 tttatctgag tcagtggcaa tccgctcgcc agagtctttg caatgtgggc actcatagat
     3421 tttggcatag ggcacatcct cgcctttgcg ccaaaggaat gcttgtgcat agatctgt
//
LOCUS       DAOREF010000233         3474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17171_length_3474_cov_3.431413, whole genome shotgun sequence.
ACCESSION   DAOREF010000233 DAOREF010000000
VERSION     DAOREF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17171_length_3474_cov_3.431413"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PKI33_06050"
     CDS             <1..301
                     /locus_tag="PKI33_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNM36602.1"
                     /translation="ILVAVLEKGQRQMRLPIIIYSVVISLMLLSAMVKLNDLNWNAGA
                     AVLVAIGAFLFYLSDIVLAWNKFVQPLQHGRIFNIALYHLGQIALIVGVVFQYRS"
     gene            345..1319
                     /locus_tag="PKI33_06055"
     CDS             345..1319
                     /locus_tag="PKI33_06055"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNM36603.1"
                     /translation="MLPRPIPVDEVRIFSGSSNAPLAQKIADELGIQLEKTHVKKFSN
                     DNLYLQLGASVRYRRVYIVQSLTPPVNDNLMELLMMLDIARDAAASEIHAIIPYYSFG
                     RSDKKDAPRISITARLVADLLRTSGATHVMSMMFHSPQVHGFFRVPTDPLSSRPVFQQ
                     YLENRDMSGMIIVSPDMGQAKSAARFAKSLSLPVAAGNKERVSDTEVRISGLVGNQVK
                     GHKRALIYDDEIATGGSIAELTRLLVNEGVEEIMVMCTHGVFTRGGLEKLAAIPQIQE
                     IVTTDTVDIPAEKRHPKLTVLSVAKVFADSIRHNFNRQSIGDLFVYGE"
     gene            complement(1370..2371)
                     /locus_tag="PKI33_06060"
     CDS             complement(1370..2371)
                     /locus_tag="PKI33_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNM36604.1"
                     /translation="MAELTFQNVLDHLLDSKKDIPAGHLAYYSDLDPKSLRLFLDVWS
                     SVPQKRKLLLLDTLVSHLDEDTIVSYEDIGKALLDDPDAEVRARAMGLLAESDDPKLI
                     DTLLKILKSDSELAPRVKASTLLGEFVLMGELEELDEARLRKIEEALIAIIRSDENPS
                     LRRAAVEAFGYSGRDEAVAILESAYEREDPLWVASALRAMGRSHDNRWDDSVVSKLLD
                     PDPRVRYAAAEAAGELTIEEAGPIMLKMLEDEEEDDNVTMAAIWSLSQIGGEDARAYI
                     LNMLDSSDDDDVTEFLEDALENLDFNDELNKFDLLSLDEDDDLSEFDEDDLDDEDDK"
     gene            complement(2375..>3474)
                     /locus_tag="PKI33_06065"
     CDS             complement(2375..>3474)
                     /locus_tag="PKI33_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNM36605.1"
                     /translation="TLVIFFLALGGMIFVHELGHFIAARWAGIEVEEFGFGLPSYKLA
                     TLFTWKGTEFTIHALPLGGFVRPKGENDPNVPGGFGAANPWKRLVVLVAGPLMNLLTA
                     IIVFSALIAYEGIAEPGKVKIDTVADNSPAQQADIQAGDIILSINGKEVSDIQPAIDI
                     IRANLDKPVEMVLDRNGEMVTVTATPLSSRTPQQGALGVGLTYPTRPATFLESIKGGT
                     TVTALQAATIVYLPIALIQGIIAPDQARLVGFKGIYDLFNYSVQEDVSSRQEAAQVQT
                     SGGGTAPKPTNFTLNLIGVLSVSLGVFNLFPIPALDGGRILFTLPEILFKKRIPTEWE
                     NMVNGVAMLLLIGLMLFVNIMDFVNPANLTP"
BASE COUNT          831 a          886 c          900 g          857 t
ORIGIN      
        1 tattttggta gctgtcctcg aaaaggggca aaggcaaatg cgcctgccca tcattattta
       61 tagcgtggtg atctcactca tgttgctttc ggcgatggtg aagctcaatg acttgaattg
      121 gaatgccggt gccgcggtgc ttgtagcgat tggagctttc ctgttttatc tttcagatat
      181 cgttctggct tggaacaagt tcgttcaacc gcttcagcat gggcgtatct ttaatattgc
      241 cttgtatcat ctaggtcaga tcgccttgat tgttggcgtt gtttttcagt atcgcagtta
      301 atttgtgtaa taatcagaac acacaaccat cttttggaga atctatgtta cctcgcccga
      361 tacccgttga tgaagtccgt atttttagcg gaagttccaa tgcgccgctt gcccaaaaaa
      421 tagccgatga attggggata caactcgaaa agacccacgt caaaaaattc agcaacgaca
      481 atctgtatct gcaactggga gctagcgtcc gctatcgacg ggtgtacatt gtccagtcgc
      541 tcacgccgcc ggtcaacgat aatttgatgg aattgttgat gatgctggat attgcccgcg
      601 atgccgccgc ttctgagatc catgccatta ttccttatta ttcttttggg cgttccgata
      661 aaaaagacgc gccgcgcatc tccatcacag cccgcctcgt tgcggacctg ctcagaacct
      721 ctggtgccac gcatgtgatg agcatgatgt tccactcgcc gcaggtgcat gggttcttcc
      781 gtgtgcctac agaccccctg agtagccgcc cggtctttca gcaatacttg gaaaaccgcg
      841 atatgagcgg catgattatc gtttcgccag atatgggtca agccaagtct gcggcgcgct
      901 ttgcgaagag cttaagtctg cctgtggcgg caggaaataa agaacgcgtc tccgataccg
      961 aagtgcgcat tagcggactt gtcggtaatc aagttaaagg gcacaaacgc gctttgatct
     1021 atgatgatga gatcgctacg ggtggctcca ttgctgaatt gacgcgtttg ctggtgaacg
     1081 aaggcgtgga agagatcatg gtgatgtgta cccatggtgt ctttacccgc ggcgggttgg
     1141 agaaactggc ggcgattccg caaattcaag aaattgtaac gaccgatacc gtcgatatcc
     1201 ctgcagagaa acgtcacccc aagctgaccg ttctttcggt ggcaaaggtc tttgcagact
     1261 ccattcgcca taacttcaac cgacagtcga ttggtgacct gttcgtgtac ggcgaataac
     1321 tctcagcgca aacaaaaaga cactcccagt aagggagtgt ctttttgttt tatttatcat
     1381 cctcatcgtc gaggtcgtct tcgtcgaatt cagaaaggtc gtcatcttca tcgagcgaga
     1441 gcaggtcaaa cttgtttagc tcatcgttga agtctaaatt ttcgagcgca tcttcgagaa
     1501 attccgttac atcgtcatca tcggagctat cgagcatgtt gaggatgtag gcacgcgcat
     1561 cttcgccgcc gatctggctc agagaccaaa tcgctgccat ggtcacgtta tcgtcctctt
     1621 cttcatcttc gagcatcttc agcataatag gacccgcttc ttcgatggtc agttcaccgg
     1681 cggcttcggc ggcagcatag cggacgcgtg gatcggggtc aagcagtttg ctgaccacgc
     1741 tatcatccca gcggttatcg tgcgaacgtc ccatggcgcg cagagcagat gccacccaaa
     1801 gcggatcttc acgctcatac gccgattcaa ggatcgccac cgcctcatca cgaccagagt
     1861 agccgaaggc ttccacggca gcgcggcgta ggcttggatt ctcgtcgctg cgaatgatgg
     1921 cgatcagcgc ttcttcgatc ttgcgcaaac gggcttcatc aagctcttcg agttcaccca
     1981 tcaacacgaa ctcgccaagc aacgtggagg ctttcacacg cggagcaagc tcgctgtcag
     2041 atttgagaat cttcaggagg gtatctataa gcttggggtc gtctgattcg gcgagcagac
     2101 ccatagctcg ggcgcggact tccgcatccg ggtcatccag aagcgctttg ccgatgtctt
     2161 catacgaaac aatggtgtct tcatcgaggt gagagaccaa ggtatctaat agaagcaatt
     2221 tacgcttttg cggaacgctg ctccaaacat ccaaaaagag gcgcaaagat tttggatcca
     2281 ggtctgagta ataggcaagg tgacccgcag gaatatcctt tttggaatca agcaggtgat
     2341 cgagtacgtt ttgaaaagta agttcagcca taagttacgg agtgaggttt gcagggttga
     2401 cgaagtccat gatgttgacg aacaacatca agccgatcag caggagcatg gcaacaccgt
     2461 tcaccatgtt ctcccattcg gtagggatgc gcttcttgaa aagaatttcg ggcagggtaa
     2521 agagaatgcg tccgccatca agcgcaggga tggggaagag gttgaagaca cccaacgaaa
     2581 cactgagcac accaatgaga ttaagcgtaa agttggtggg cttgggggct gtgccaccgc
     2641 cgcttgtttg tacttgtgcg gcttcttgtc ggctcgatac atcttcttgt accgaataat
     2701 tgaacaggtc gtagatgcct ttgaacccaa ccaaccgtgc ctggtctggc gcgatgatgc
     2761 cttgtatgag cgcaatcggt agatacacga tcgttgcagc ttgcaaagct gtgaccgttg
     2821 taccgccctt gatgctctca aggaaggtgg ctgggcgagt ggggtacgtg agcccaacac
     2881 ccaaggcacc ttgttgcggt gtgcggctgg aaagtggtgt tgccgtaacc gttaccatct
     2941 caccattgcg gtccaagacc atttccaccg gcttatctaa attcgcgcgg atgatatcaa
     3001 tggcgggttg aatgtcgctc acttccttcc cattaatgga aaggatgatg tcaccggctt
     3061 gaatatctgc ttgctgtgcc ggagagttat ctgccacggt atcgatcttg accttgcccg
     3121 gttcggcgat gccttcgtaa gcgatcaatg ccgaaaacac aatgatggca gtcagaagat
     3181 tcattaacgg tcccgccacc aaaacgacca aacgcttcca cgggttggca gcgccaaaac
     3241 cgcccggcac gttcgggtca ttctcgcctt tggggcgcac aaacccgccc aatggcaggg
     3301 catggatcgt aaactcagtt cccttccagg taaacaatgt tgccagttta tacgagggca
     3361 agccgaaacc aaattcttcc acttcaatgc ctgcccaccg cgcagcgata aaatgtccaa
     3421 gttcatggac gaagatcatc ccgccgagcg ccagaaaaaa gatgaccaac gtca
//
LOCUS       DAOREF010000234         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17191_length_3471_cov_4.689988, whole genome shotgun sequence.
ACCESSION   DAOREF010000234 DAOREF010000000
VERSION     DAOREF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17191_length_3471_cov_4.689988"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1062)
                     /gene="arcC"
                     /locus_tag="PKI33_06070"
     CDS             complement(109..1062)
                     /gene="arcC"
                     /locus_tag="PKI33_06070"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNM36606.1"
                     /translation="MTNLAVIAVGGNSLIIDDKNVTVESQYQAAKQTCLHIADLIEQG
                     WDVAIGHGNGPQVGYILRRSEIAAKTAGMHEVPLDVCGADSQGAIGYAIQQNLQNILY
                     QRGIKKKVVTVITQTLVDKTDPAFSKPAKPIGSFMDKEEAERRQQEQGWSVVEDAGRG
                     WRRVVASPLPKEIVELETVQTLLEKGIITITVGGGGIPVVDPGDGNYEGVAAVVDKDY
                     ASSLLARSIGANLFVISTAVEKVAINFGKPEQKWLDQITLAEAKAYLAEGIHFAKGSM
                     APKIQAIIWYLEAMPNGKALITNPENIGRALKGETGTWIVP"
     gene            complement(1126..2106)
                     /locus_tag="PKI33_06075"
     CDS             complement(1126..2106)
                     /locus_tag="PKI33_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNM36607.1"
                     /translation="MQTNFRGRDFIGDLDFSKEEVETVLDVAWDLKRKRALGESHALL
                     RDKTLAMLFFFTSTRTRGSFEAGMAQLGGHAAFIDSETTQISHGDTAKEIGEIFGRYF
                     DGIAIRQCDWNFGNQYINDVAKASRVPILNMQCDVYHPFQCLADLMTIQEKKGRDLKK
                     KKVVVSWAYAASYSKPISVPQSLILQMTRFGCDVVLAHPPEFKLMPEIMQQAKDNAKK
                     YGVGFDVTDNMDEAFKDADVVYPKSWGPMLTTTGKEEGKALIEKYKDWITDERRMKLT
                     KDDSIYMHCLPADRGLEVTDGVIDGPHSVVYDEAENRLHAQKAVMALTMS"
     gene            complement(2153..3127)
                     /locus_tag="PKI33_06080"
     CDS             complement(2153..3127)
                     /locus_tag="PKI33_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNM36608.1"
                     /translation="MRKVLPVLFILTMLLLACGSNTAKPKVALMLARGGLGDKAFNDS
                     ANEGLQKAAQELNAEVTTFDYAEDRVEANIRQAAEDGYSLIIGLGSENSQAIATVAKE
                     FPETKFAIIDATAEGDNVTSVTFSELEGDFLAGALSALMSENGKVGYLGGADVLVIRR
                     IQYGFEQGVKYINPDAEIVVQYIGGKDDFSGFAKPDEAQTIAAQMYGDGVDIIYAAAG
                     GSTLGAIKAAESAGKPIVTTGSDQRYLAPNVVATSRTKNMNEAVFMLIQKFVDDSLPS
                     GTIQLDYKSGGIGLAPLADFVPASVSEAFLFVKSDIEDGAIKVEPFTE"
     gene            complement(3124..>3471)
                     /locus_tag="PKI33_06085"
     CDS             complement(3124..>3471)
                     /locus_tag="PKI33_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36609.1"
                     /translation="LMNTYGGAVEREAPWPMPFFHYSEIVKQAEAAAKYAHDLMDYYL
                     GILNHIPQGGSALVVNHGGVVELGVVACLPDADFSAWGDAVEYCEGARLFWDGGKFVN
                     GEVLRLMYNKESL"
BASE COUNT          852 a          941 c          796 g          882 t
ORIGIN      
        1 ccgtactgcg cggtgtaaat tggatgttgt tcaaacggtc agtcaatttt ttcattagaa
       61 caattgtaaa cgaatccgct gccgaaattg gcagcggaat tacatcgtct atggaacaat
      121 ccaggtaccg gtttcgcctt tgagcgcgcg cccaatattt tcggggttgg tgattagagc
      181 tttaccattc ggcatggctt cgagatacca aataatcgct tggatctttg gcgccatgga
      241 gcctttggca aaatggattc cctcagccaa gtacgctttg gcttcggcta gtgtgatctg
      301 gtccagccat ttttgctcgg gtttaccaaa gttgatggca actttttcca ccgccgttga
      361 aatgacaaat agattcgcac caatagagcg ggctaacaaa gaagaggcgt aatccttgtc
      421 aaccacagcc gccacgcctt cataatttcc atccccaggg tcaacgaccg gaatgccacc
      481 accaccaact gtaatggtga tgattccttt ttccaacagg gtttgaacgg tttccaattc
      541 cacgatttct tttggcaaag gcgaggcgac gacccgcctc caaccacgac ctgcatcttc
      601 caccacagac cagccttgtt cctgctgacg gcgttccgct tcttctttat ccataaagct
      661 gccaatgggt ttggcgggct tggaaaacgc tgggtcagtt ttatctacca aggtttgggt
      721 gatgacagta acaacctttt tcttgatgcc acgttggtag agaatgttct gcaaattttg
      781 ttgaatggcg tacccgattg cgccctggct atcagcaccg cacacatcca aagggacttc
      841 gtgcatgcca gccgttttgg cagcaatttc agagcggcgc aaaatataac caacctgcgg
      901 tccattgcca tgtccaatcg ccacatccca accttgttcg atcaaatccg caatatgtaa
      961 acaggtctgc tttgccgcct ggtattgact ttcgacagtc acgtttttat catcaataat
     1021 gagcgagtta ccgcccactg caatcacagc aagatttgtc atctataaag tctcctgagc
     1081 aaaagactag cagggacaca ctggagtgtg cccctgcaaa ttaacttaag acatcgtcaa
     1141 agccatgacc gccttctgag catgcagacg gttttcagct tcgtcatata ccaccgagtg
     1201 cggaccgtcg atcacgccat cggtcacttc caaaccgcgg tcagcgggca gacagtgcat
     1261 atagatggag tcatccttgg tcagcttcat gcggcgttcg tcggtgatcc agtccttgta
     1321 cttttcaatc agtgctttgc cttcttcctt acccgtggtg gtcagcatag gaccccaact
     1381 cttggggtag accacatcgg catccttgaa agcttcatcc atgttgtcgg tcacatcgaa
     1441 gccaacgcca tatttcttgg cattgtcttt cgcctgttgc atgatctcag gcataagctt
     1501 gaactctggt ggatgcgcca acaccacatc gcagccaaag cgggtcatct gaagaattag
     1561 agattgtggc acagagatgg gcttggaata ggaagccgca tacgcccaag aaaccaccac
     1621 cttctttttc ttgaggtcac gtcccttctt ttcttgaatg gtcatcagat ccgcaaggca
     1681 ttggaagggg tgatagacat cgcattgcat gttaaggatc ggcacgcggc tggctttggc
     1741 aacatcattg atgtattgat tgccgaagtt ccagtcacat tggcggatgg caatgccgtc
     1801 aaaatagcgt ccgaagatct caccgatttc cttggcagtg tcgccatgag agatctgtgt
     1861 ggtttcgctg tcaataaagg cagcatgacc acccaactgc gccatgcccg cttcgaacga
     1921 cccgcgggtg cgggtggacg tgaagaagaa caacatagcc agagtcttgt cgcgcagcaa
     1981 ggcatgagat tcacccaagg cgcgtttgcg ttttagatcc cacgccacat ctaaaacggt
     2041 ttcgacttct tctttcgaga agtcgaggtc gccgataaaa tcacgaccac gaaaattcgt
     2101 ttgcataatt tatctcctta tcttttctag ttggtcgtta aaccaacctt tattattctg
     2161 taaacggctc aaccttgatg gcaccgtctt caatatccga tttcacgaag agaaaagcct
     2221 cagaaacaga agcaggcaca aagtcggcaa gaggcgcaag accaatgcca ccagacttgt
     2281 aatccagttg gatcgttccg cttggcaggc tgtcatccac aaatttttgg atcagcataa
     2341 agacagcctc gttcatgttc ttggtgcgac tggtcgccac cacattggga gctaaataac
     2401 gctggtcgct tccggtcgtg acgatgggct tgcctgcgct ttcagccgcc ttgatcgccc
     2461 cgagagtgga cccacccgcc gccgcataaa taatatcgac accgtcacca tacatttgag
     2521 cggcaatcgt ttgagcttca tcaggtttgg cgaagccgct gaaatcatct ttgccgccga
     2581 tatactgaac aacaatttca gcatcggggt tgatgtattt cacgccttgc tcaaagccat
     2641 actgaatacg gcggatcaca agcacatctg ccccgcccag ataaccaacc ttgccattct
     2701 cactcatcaa agcagataaa gctcctgcaa gaaaatcgcc ttcgagttca ctgaatgtga
     2761 ccgaggtgac gttgtcgcct tctgcagttg catcgatgat ggcgaacttc gtttcaggaa
     2821 attctttggc aaccgtagca atggcttgtg aattttcaga acccagtcca atgatgaggc
     2881 tatacccatc ttctgccgct tggcggatgt ttgcctctac acgatcttcg gcatagtcaa
     2941 aggttgtgac ctctgcattt aattcttgag ccgctttttg caaaccttca ttcgcactat
     3001 cgttaaaggc tttgtcgccc aagccgccgc gcgccaacat cagagccact ttgggtttgg
     3061 ctgtgttgct gccgcatgct aaaagcaaca tggttaagat aaaaagaacc ggtaaaactt
     3121 ttctcacaaa ctctccttgt tatacataag ccgcaacact tcgccgttca caaatttgcc
     3181 gccatcccaa aagagacggg cgccttcgca atactccacg gcatcacccc atgcagaaaa
     3241 atctgcatcg ggcaggcatg ccacaacacc cagttccacc acgccgccgt ggttcaccac
     3301 caacgccgaa ccgccttgcg gaatgtgatt gagaataccc aggtagtaat ccatcaagtc
     3361 atgtgcgtac ttcgcagccg cctctgcctg cttgacgatc tctgaataat ggaagaacgg
     3421 catgggccag ggagcttctc gctctacggc gccaccgtac gtattcatga g
//
LOCUS       DAOREF010000235         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17262_length_3463_cov_4.610035, whole genome shotgun sequence.
ACCESSION   DAOREF010000235 DAOREF010000000
VERSION     DAOREF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17262_length_3463_cov_4.610035"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..773
                     /locus_tag="PKI33_06090"
     CDS             243..773
                     /locus_tag="PKI33_06090"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNM36610.1"
                     /translation="MPSFELIRSEPLMQGRAFKIRRDYLKTPDGHETKYDIVEHGGSV
                     VIVPFDGDGNLLFVRQYRHAAGMDMLELPAGTRDGDEPFEECAAREIREETGMEAGKL
                     IHIGSFYLAPGYSTEYMGVFLATDLKHNPLEADEDEFLSVEKIPVKDALKMAEKGEMP
                     DAKSLAALLMARAYLE"
     gene            978..>3463
                     /gene="nifJ"
                     /locus_tag="PKI33_06095"
     CDS             978..>3463
                     /gene="nifJ"
                     /locus_tag="PKI33_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNM36611.1"
                     /translation="MNKNIIMIDGNEATASVAHRLNEVIAIYPITPSSAMGEFSDEWS
                     AKGKKNIWGTVPLVVEMQSEGGAAGTVHGALQTGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARAIAAHALSIYGDHQDVMAVRQTGWAMLSSGSVQEAQDFAAISQAAT
                     LKARVPFLHFFDGFRTSHEVNKIIQLSDEELRSMLDDDLIRAHRARGLSPEHPVLRGT
                     AQNPDVYFQMREAANPYYAAVPAITQEMMDKFAKITGRHYNLFDYSGDPNAERVIIIM
                     GSGGEVVEETVNYLAQKGEKVGVLQIRLYRPFSVEHFIKALPKSVKSLAVLDRTKEPG
                     AMGEPLYMDVVSALVEGQRSNIKVIGGRYGLSSKEFTPAMAKAVFDELAKPEPKNHFT
                     VGINDDVSNTSLTVDSSFSIENEGMVSCVFFGLGSDGTVGANKNSIKIIGEETDNFAQ
                     GYFYYDSKKSGTITMSHLRFGPKAIRAPYLIEASQANFVACHQYSFLERVDMLKYAKE
                     GGIFLLNSLYGPEEIWDQLQQEVQKDIIEKKLKFYVINGYDVAERTGMGGRMNTIMQT
                     AFFAISGILPKEQAIEQIKYAIKKTYGKRGEAVVAKNFEAVDATVANIYEVKVPAKVS
                     SKTVRRLPVPNEAPDFVKNVLGQIIDSDGDNIPVSAFPVDGTFPTGTTQWEKRNLALE
                     IPVWNEDICIQCGKCVLICPHAVIRHKVYEDASLENAPETFKHTVSKFKEFSSGYSYT
                     VQVAPEDCTGCSLCVEICPVKDKTAVGRKAINMEPQPALREAEAKNWDFFMNIPDLDR
                     KLINPSTIKNAQLLRPLFEFSGACAGCGETP"
BASE COUNT          945 a          880 c          820 g          818 t
ORIGIN      
        1 tcgcgttttg atcgcgcacc cggcttcgta cacagtcgct tcgggtgatg aaacagtgta
       61 cagcatcgct tgtaaatatg gtgatgtaga cccgaatgca ctcgcgagtg cgaatggaat
      121 ttctgtctct tccaagttga cggttgggca ggttctgagc attccgtaat ttcaaaaaga
      181 caaaagccca ggtcaacagc ctgggctttt tttatagaaa ctattcttga aaggaaatca
      241 ctatgccatc atttgaactc attcgttctg aacctctcat gcagggacgt gccttcaaga
      301 ttcgccgtga ttatttgaaa acaccagatg gtcacgaaac caaatacgat attgtagaac
      361 atggcggctc ggtggtgatc gtgccatttg acggtgatgg gaacctgctc ttcgtgcgac
      421 aataccgtca cgctgctggt atggatatgc ttgaactgcc cgctggtacg cgcgatggtg
      481 atgaaccatt tgaagaatgt gccgcacgtg agatccgcga agagacaggc atggaagctg
      541 gcaagttgat tcacatcggt tcgttctacc tcgcaccggg ctactccact gaatacatgg
      601 gcgtcttctt agcaacagat ttgaaacaca atccactaga agccgatgaa gatgaattcc
      661 taagcgtaga aaaaattccc gtcaaagatg cattgaaaat ggcagagaaa ggcgaaatgc
      721 cagatgcaaa atcattggca gcattgctaa tggcaagagc gtatcttgaa taaatcattt
      781 gaaaaataac aatcgctcaa aaaacgtcaa aacacctcgc aaacgtcgag gtgttttatt
      841 tccaccaatc ctatccaata tatgacatca ggcaggggga tttaagtcat atataactaa
      901 cgtatttcat cccgcaggaa taaacttatt cattggaaaa tacgaggcat tcgtgccttt
      961 tttacaggag caaaagcatg aataagaata tcattatgat cgacggaaac gaagccacag
     1021 ccagtgtagc ccaccgtctc aatgaagtga tcgccattta cccgatcacc ccatcctctg
     1081 cgatgggtga attctcagac gaatggtcag ctaagggcaa aaagaacatt tggggaaccg
     1141 ttccactcgt ggttgaaatg caatctgaag gcggcgcagc cggaaccgta cacggcgccc
     1201 tccaaaccgg tgctcttacc accaccttca ccgcgtctca aggcttattg ttgatgatcc
     1261 ccaacatgta caaaattgcg ggcgaactca ccagcaccgt cttccacgtg gcagcgcgcg
     1321 ccattgcagc ccacgctctt tccatttatg gcgaccatca agatgttatg gctgtccgcc
     1381 aaaccggttg ggcaatgctc tcttctggta gcgtgcaaga agcacaagat tttgccgcaa
     1441 tctctcaagc cgccaccctc aaggcacgcg tgcccttcct tcatttcttt gatggattta
     1501 gaacctcgca tgaagtgaac aagatcatcc aattatcgga tgaagagctc cgcagcatgc
     1561 tggacgatga cctgattcga gcccatcgag cccgcggact cagccctgag catcccgtct
     1621 tacgcggtac tgcccagaac cccgatgtct acttccaaat gcgtgaagcg gcaaacccat
     1681 actacgctgc cgtccctgcc atcacgcaag aaatgatgga taagttcgcc aagatcactg
     1741 ggcgtcacta taacctgttc gattactccg gcgacccgaa tgccgaacgt gtcattatca
     1801 tcatgggttc tggcggcgaa gtagtcgaag agaccgtcaa ttatcttgcg caaaaaggcg
     1861 aaaaggtcgg tgtcttgcaa attcgcttgt accgcccatt ctctgtggag cacttcatta
     1921 aagcgttgcc caagagcgtg aaatctctcg ccgtgttaga ccgcaccaaa gaacctggcg
     1981 ccatgggcga acctctttac atggacgtgg tcagtgcctt ggtagaaggt caacgctcca
     2041 atatcaaagt gattggtggt cggtatgggt tgtcttccaa agagtttact cccgccatgg
     2101 caaaggcagt ttttgacgaa cttgccaagc ccgaacccaa gaatcatttc accgtcggta
     2161 tcaatgacga tgtctccaac acttcactca cagtagactc ctccttctca atcgaaaatg
     2221 aaggtatggt cagttgtgtc ttcttcggtc tcggttcaga cggaactgta ggtgcaaaca
     2281 agaactccat caagatcatc ggtgaagaga cggataactt tgcccaaggc tacttctatt
     2341 atgactcgaa gaaatcgggc acgatcacta tgtcacactt gcgctttggt cccaaagcca
     2401 ttcgtgcgcc gtatctgatc gaagccagcc aagcgaactt tgtagcttgc catcaatact
     2461 ccttcctcga acgcgtagat atgctcaagt atgccaaaga aggcggcatc ttcctactca
     2521 acagtcttta tggtcccgaa gagatctggg atcagctcca acaggaagta caaaaagaca
     2581 ttattgagaa gaagctcaag ttctacgtta tcaacggcta tgatgtagcg gaaagaaccg
     2641 gtatgggcgg acgcatgaac accatcatgc agacagcatt cttcgctatt agcggtattt
     2701 tgccaaaaga gcaagccatt gagcaaatca agtatgccat caagaagact tatggcaaac
     2761 gcggcgaagc cgtagtggca aagaactttg aagctgtgga tgcgaccgtg gcaaatattt
     2821 atgaggtcaa agttccagcg aaggtaagct caaagaccgt acgcagactc ccggtcccga
     2881 atgaagcgcc tgacttcgtc aagaatgtac tcggtcagat catcgactca gacggcgaca
     2941 acatccccgt ctctgccttc cccgtagacg gcaccttccc caccggcact acgcaatggg
     3001 aaaagcgcaa tctcgcgttg gaaattccgg tttggaatga agatatttgt attcaatgcg
     3061 gtaagtgtgt gttgatctgc ccgcatgcag ttattcgtca caaagtgtat gaagatgcaa
     3121 gtttggaaaa tgcccccgaa acgttcaagc atactgtttc caagttcaag gaattttcga
     3181 gcggatactc ctacacagtg caagttgcgc cagaagattg taccggctgt tcgttgtgcg
     3241 tggaaatctg cccagtgaag gataaaacgg cggttggacg caaggcgatc aacatggaac
     3301 cgcaacccgc tttgcgcgaa gccgaagcta agaactggga tttcttcatg aacatccctg
     3361 atcttgaccg caagttgatc aacccttcaa ccatcaagaa cgcccaactg ctacgcccgt
     3421 tgttcgagtt cagcggtgct tgtgccggct gtggtgagac ccc
//
LOCUS       DAOREF010000236         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17277_length_3461_cov_4.007340, whole genome shotgun sequence.
ACCESSION   DAOREF010000236 DAOREF010000000
VERSION     DAOREF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17277_length_3461_cov_4.007340"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..2134
                     /gene="uvrC"
                     /locus_tag="PKI33_06100"
     CDS             263..2134
                     /gene="uvrC"
                     /locus_tag="PKI33_06100"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNM36612.1"
                     /translation="MEVSEKLQGILATLPTKPGCYLYRNAEGTIIYVGKAINLKNRVR
                     SYFHADSSHDNKTRRLVRDIADIEWIVVGSELEALILEMNLIKKHRPKYNIRLKDDKR
                     YPYIKVHWNEPFPKITVTRQMEEDGSRYFGPYTSAWAVYQTLEVLRKIFPYLTCDREI
                     TGNDPRACLYYDIKLCTGPCIGAVSKDGYRQMISDLMEFLSGHSEPIVERVQADMLKA
                     SEEMRFEKAAALRDQLKAMQSIIERQKIVFGSDYADSDVIAMAREDGEACVQIFFIRG
                     GKLIGREYFILEGTEDTTDSQVMEEFVKQFYTEAANIPEQVMLPQELEERKIIAQWLR
                     SKRGGEKVELFVPNEGQPKDLVQMATENATETLSSLRAQWQADAHRQEQALSDLQTHL
                     NLSAPPNRIECYDVSHTQGVATVGAMVVFEQGVPAKRLYRKFNIESTSIGNPDDFASM
                     EEMLTRRFRRWKGSQETESEVGSKKDEAFSFLPDLIIIDGGKGQLGRVVKVMEQFEIF
                     DKVPVVGLAKQEEEIFFPHKSDPLLLPRHSQGLYLVQRIRDEAHRYGITAHRKKRSKL
                     GLASQLDSIPEIGPTRRKALLKHFGSMDKIKEASVEELMTVKGMTETAAKNVKAHLE"
     gene            2131..2511
                     /locus_tag="PKI33_06105"
     CDS             2131..2511
                     /locus_tag="PKI33_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36613.1"
                     /translation="MKEVWIVDDDEEMARAVSLMLKLLDCTSTHYLGPRPAAQALLSG
                     KRPDMMILDINMPEVTGLDMLEFLRRRPEWKNLPVIMLSSEAADVMVDKALKLGADGY
                     VMKPVTIEELEKVMAQAFYKNIIK"
     gene            2521..2670
                     /locus_tag="PKI33_06110"
     CDS             2521..2670
                     /locus_tag="PKI33_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36614.1"
                     /translation="MAGKNSKNQKNNKVMEEKVAKRQKTAQIMFAAFAILLIISMVLS
                     AVAKY"
     gene            2784..>3461
                     /locus_tag="PKI33_06115"
     CDS             2784..>3461
                     /locus_tag="PKI33_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNM36615.1"
                     /translation="MLDKLQAIEEKFEQLGQELLVVGTDYKRAAEISKERTSLEPLIE
                     KAKEYRQAVKSLEEAKEMLISESDAELLALAKADIADLEPKIEELEKVIKGLLVPKDP
                     RDDKNVIVEIRAGAGGDEAAIFAADLFRMYTRYTDEKKWKTEIMSENAIGVGGYKEVI
                     FEVKGKGAYSKLKYESGVHRVQRVPATESAGRIHTSTATVTVMAEVEEVDVVIPETDI
                     EIEVYRSS"
BASE COUNT          985 a          812 c          869 g          795 t
ORIGIN      
        1 ctttcagaga tatcaataaa cgtttgttca tttggttttg taactgcttt gatccatgtt
       61 tgaacccagc ctgcgggtcc ggggttggat tccattacag gtgtgtttga aggcatttgt
      121 tccatttgaa ttctcctttt tctttccaaa gtgatgacgt atccatttta agcgaagata
      181 ttccgcttga ccgtactgta tttcaacggc aattttcaga acaaatatcc taatttcgat
      241 aacaagcggt agaattgccg caatggaagt ttcagagaag ctacaaggca ttcttgccac
      301 cctgcccacc aaaccgggct gttatctata tcgcaacgcc gaagggacaa tcatttacgt
      361 cggcaaggcg atcaatttga agaaccgcgt acggagctat ttccacgccg attctagtca
      421 cgataacaag acccgccgcc tggtacgcga cattgcagat attgaatgga tcgtggtcgg
      481 ctcagagctt gaggcactca tccttgagat gaacctcatt aagaagcatc gtccgaagta
      541 caacatccgg ctcaaagacg ataaacgcta tccatacatc aaagtccatt ggaatgaacc
      601 ttttccaaaa atcacggtca cccgccaaat ggaagaagat ggctcgcgtt attttggtcc
      661 atatacctcg gcatgggcag tgtatcagac gctggaagtg ctgcgaaaaa tattccccta
      721 cttgacctgt gaccgcgaga tcacaggcaa tgatccacgg gcatgtttgt attacgacat
      781 taagttgtgt acaggtccgt gcattggggc ggtttcaaaa gatggctacc gccaaatgat
      841 ctcagacttg atggaattcc tcagcggaca cagcgaaccc attgtggagc gcgtccaagc
      901 agacatgctc aaagcatctg aagagatgcg ttttgaaaaa gccgccgctc tgcgagacca
      961 gttgaaggcg atgcaatcca tcattgaacg gcagaaaatt gtcttcgggt cagattatgc
     1021 ggactcagat gtcatcgcga tggcgcgcga agatggcgaa gcctgcgtgc aaatattctt
     1081 catccgcggc ggcaaattga tcgggcgcga gtacttcatc cttgaaggga cagaggatac
     1141 gaccgatagc caagtgatgg aagaattcgt caaacagttc tataccgaag cagcaaacat
     1201 ccctgaacaa gtgatgttgc cgcaagaatt ggaagaaaga aagatcatcg cgcaatggtt
     1261 gcggtcaaaa cgtggcggcg aaaaagtgga attgttcgtg ccaaatgaag gtcagcccaa
     1321 agatttggtg cagatggcaa ccgaaaatgc caccgagacc ttgtcctctt tgcgggcgca
     1381 atggcaagcc gatgcgcacc gacaagagca ggcgctttct gatttgcaaa ctcatttgaa
     1441 tttatctgcc ccgccaaatc gcattgagtg ttacgatgtg agtcacaccc aaggcgtagc
     1501 aacggtcggc gcgatggttg tgttcgaaca aggtgttccg gcaaaacggc tgtatcgtaa
     1561 gttcaacatt gaaagcacca gcataggaaa cccggatgac tttgcatcga tggaagaaat
     1621 gctcacccgt cgcttccggc gttggaaagg ctcacaggaa accgagtcag aagtcgggtc
     1681 caagaaagat gaggcgtttt ccttcctccc agacctgatt attatcgatg gcggtaaagg
     1741 tcaattggga agagtcgtca aagtaatgga gcaatttgag atcttcgata aagtgccggt
     1801 tgtgggtctc gccaaacaag aagaagagat tttcttcccg cacaaaagtg acccgctgct
     1861 cctcccccgc cactcacagg gactctacct cgtgcagagg attcgcgacg aagcccatcg
     1921 ctacggaatt accgcgcatc gcaaaaaacg ctcgaaactg ggtcttgctt ctcaactgga
     1981 ttccatcccg gaaattggtc ctactagaag gaaggcgctc ttgaaacatt tcgggtctat
     2041 ggataagatt aaagaagcga gtgtcgaaga attgatgaca gtcaaaggca tgaccgagac
     2101 tgccgcgaaa aatgtaaagg ctcatttaga atgaaagaag tttggatcgt agatgacgac
     2161 gaagagatgg ctcgcgccgt cagtttgatg ctaaaactgc tggactgtac ctccacacac
     2221 tatctgggtc cgcgcccggc agcgcaggca ttgctctcag gcaagcgacc agacatgatg
     2281 atcttggaca tcaacatgcc cgaagtgacc ggcttggaca tgctggaatt cctacgccgc
     2341 cgccctgaat ggaagaattt accggttatc atgctctcct ctgaagccgc cgatgtgatg
     2401 gtggataaag ccctcaagct cggcgcagac ggatatgtga tgaaacctgt tacaatcgag
     2461 gaacttgaga aggtaatggc tcaagcattc tacaaaaata tcataaagtg agtagttgaa
     2521 atggctggaa agaattcaaa aaatcaaaaa aataataagg tgatggaaga aaaggtcgct
     2581 aagcgtcaaa aaacggcaca gattatgttt gctgcttttg ccattctttt gatcatctcc
     2641 atggtcctct ctgcagtcgc aaaatattag cttggtttca tcgtaagacc tctttgcgcg
     2701 gcgcctcttc gttatggtgc cgcgctttct tgtgcaaatt tacaaaaccg accgcagacg
     2761 atatcgtcaa gacttgagga attatgttag ataaattaca agccattgaa gaaaaatttg
     2821 aacagcttgg gcaggaactg cttgtcgttg gcacagacta caaacgtgcc gccgagatta
     2881 gcaaggaacg caccagcttg gaacccttga tcgaaaaagc aaaggaatat cgccaggcag
     2941 tgaagagctt ggaagaagcc aaggaaatgc ttatctctga aagcgacgcc gaattgctcg
     3001 ctctcgccaa agcagatata gcagacctcg agcccaagat cgaagaactt gaaaaagtta
     3061 tcaagggctt gcttgtgccg aaagacccac gtgatgataa gaacgtcatc gtggaaattc
     3121 gcgcaggcgc aggcggtgac gaagccgcca tcttcgccgc agatctcttc cgcatgtaca
     3181 cccgctatac agatgagaaa aaatggaaga cggagatcat gtctgaaaac gccattggcg
     3241 tcggtggata taaagaagtc atcttcgaag taaagggcaa aggcgcttac tccaagctga
     3301 aatatgaatc gggtgtgcat cgcgtacagc gcgtccccgc aacggaatcc gctgggcgga
     3361 ttcatacctc taccgccacc gtcaccgtta tggcggaagt cgaagaagtg gatgtcgtca
     3421 tccctgaaac cgatatcgag atcgaagtct atcgttcgtc t
//
LOCUS       DAOREF010000237         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17287_length_3460_cov_4.637885, whole genome shotgun sequence.
ACCESSION   DAOREF010000237 DAOREF010000000
VERSION     DAOREF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17287_length_3460_cov_4.637885"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKI33_06120"
     CDS             complement(<1..481)
                     /locus_tag="PKI33_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNM36616.1"
                     /translation="MTKTVAIIGGGPAGLMAAEVLSQNADLQIDVYDAMPSLGRKFLM
                     AGKSGLNLTHSEPFEKFVSRYSRKEVEAWLHAFTPDNLRTWAKELGVDTFVGTSGRVF
                     PVGMKASPLLRAWIQRLSASGVTFHLRHKLSGLPADTSLHFETPHGDVTVKADAVILA
                     "
     gene            complement(537..704)
                     /locus_tag="PKI33_06125"
     CDS             complement(537..704)
                     /locus_tag="PKI33_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36617.1"
                     /translation="MILNRTTRNILIAISAGVATGIFLNMLSMSLPSLIVGVVVATGV
                     TFALVHSRKDS"
     gene            complement(710..1771)
                     /locus_tag="PKI33_06130"
     CDS             complement(710..1771)
                     /locus_tag="PKI33_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNM36618.1"
                     /translation="MQRRSKGVVQLEWVCPNCDTRNPGPVKNCQNCGAAQPENVQFQR
                     AADEKLITDEKVVQAAKAGADIHCGFCGTRNPATAETCSQCGADLKEGKARQAGQVMQ
                     AAPSTPKVVNCSNCGSENSGSAKTCTNCGAPIKLPQAKAPAPIAASAQAGKVAAQANK
                     KPVNWLVAGGIGAVLVMCCIAVLMLFVFPSRSVTGTVSGVSWQTSVPVQEVQPVNYSN
                     ERGNPPSDAYNVSCRTDSQEVCEDKTIDKGNGYAEVVTECHTVKDQYCSYTVDEWTTI
                     QTYDLNGTDLNPEYSSPNISSDQRLGNSSTQLQVYFSTSDGELTYSPNSVTEFQQYQP
                     GSQWTLKLNAVGSVLSVER"
     gene            complement(1895..>3460)
                     /locus_tag="PKI33_06135"
     CDS             complement(1895..>3460)
                     /locus_tag="PKI33_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM36619.1"
                     /translation="YAADFVEYLTTPDTAGLEGLDTPEEQKTAIDDSRAYAQNCIAEM
                     GIDPAKLAFYGTDQVAEDIDSFRALLGDEKLWIYGVSYGTAVAQTYATEHPDRVAGMI
                     LDGTINLTLDGVQGSLAQEKAFDKVLVAVLNACDEDEVCHADMGGENALAVYDKFAAQ
                     VSKDPIEYEYPLPNGEKVKGVFTFNALEFTTAYQMYSLTTRMIFLRALAAANQGDIIP
                     MLRLMYENATIDPATFEYIGDPTFSDTMFLSVLCTDDTFFTGTQEERIQQSIEAGQAS
                     NGTVPRLDGSVYTGIDCAFWPSSPKEEVTREPLKLDGVPVLVLNATLDPATPFEEGKF
                     VAENLADGYHIYVEGGVHSIFGYGYACPDQYVTDLLVDGKVPDQREIQCTDWENPVYA
                     NYSPNLPASANDFADALDMMIYLDENFYYLPEVYLNDWSEEKSIGCTYGGTYTFAPSE
                     KGEDHTYENCQMMPGVTVNGTGTFDYNAGVFSMAVNISGDKTGELNYSYDFNTGTTSV
                     TGTFEGEPVDLSQ"
BASE COUNT          816 a          857 c          874 g          913 t
ORIGIN      
        1 aggcaaggat gaccgcatcg gctttcacgg tcacatcacc atggggtgtc tcaaaatgta
       61 gagacgtatc ggctggcaac ccgctcaatt tgtggcgcag gtgaaaggtc acgccagagg
      121 cgctgagtct ttgaatccac gcccgcagta gtgggctggc tttcatccca accggaaaga
      181 cgcgccctga agttcccacg aacgtatcta caccgagttc ttttgcccag gtacgcaaat
      241 tatcgggcgt gaaggcatgc agccaagctt cgacttcttt gcggctgtag cgcgaaacga
      301 atttttcaaa aggttcagag tgggtaaggt ttagcccgct tttgcctgcc attaagaatt
      361 tgcgccccag cgaaggcatg gcatcatata catctatttg aaggtcagcg ttttgtgata
      421 agacctctgc tgccattaag ccagctggtc cgccgccgat gattgctacg gttttcgtca
      481 tggggttcat tataaagaga aactcccgaa agggagtttc tctttatata taacgattaa
      541 ctatctttgc gagaatgcac gagcgcaaat gtcacacccg tggcaacgac aacgcccacg
      601 ataagcgaag gcagcgacat ggatagcatg ttaaggaaga tgccggttgc aaccccggct
      661 gaaattgcga tcaagatatt tcgggtcgtt ctattgagaa tcatagggct tatcgttcta
      721 cgctgagcac actgcccacg gcatttaact tcaacgtcca ctgactgccg ggttgatatt
      781 gttgaaactc agtgaccgag ttgggcgaat aggtcaactc gccatcgctg gtgctgaaat
      841 atacctgtaa ctgggtagag ctattcccga gacgttgatc gctggaaatg ttggggctgg
      901 aatattcagg gttcaggtct gttccattta ggtcataggt ctgaatggtt gtccactcgt
      961 cgacggtgta actgcaatat tggtctttta cggtgtgaca ttctgtcact acttcagcgt
     1021 agccattgcc tttgtcgatg gtcttgtctt cgcagacttc ttgactatcg gttcggcagg
     1081 aaacgttata cgcatcagag gggggatttc cgcgttcgtt tgaataattg accggttgaa
     1141 cttcttgcac tggaaccgat gtttgccaag agacaccaga gactgtgccc gtgacagaac
     1201 gagaggggaa cacgaacaac atcaaaacag cgatacagca catcaccaaa acggcgccaa
     1261 tgccgcctgc caccaaccag ttgactggtt ttttatttgc ctgcgcagca accttgcccg
     1321 cttgtgcaga tgccgcgatg ggggcgggcg ctttggcttg tggcaatttg atgggcgcac
     1381 cacagttcgt acaggttttg gcactgcctg agttttcgct tccgcaattc gaacagttga
     1441 caactttcgg cgtggaagga gccgcttgca tcacttgccc agcctgacgg gctttgcctt
     1501 ccttcaaatc cgcgccgcat tgcgaacaag tctctgctgt ggcggggttg cgcgtcccac
     1561 agaatccgca gtggatgtct gcacccgctt tggcagcttg caccaccttt tcatcggtga
     1621 tcaatttttc atcagcggct cgctggaatt ggacattttc aggttgggcc gcgccgcagt
     1681 tctggcagtt cttgacgggt cccgggttgc gcgtgtcgca gtttgggcat acccattcca
     1741 actgcacaac gcctttactt cttctttgca tggtctactc cttatcaatc attgcgtatg
     1801 tgccaagtgt acaacaatct gtgcatggat tgaatggcat cattaaaaac accttcgagg
     1861 gtatcgaagg tgtttaggca tcgcaattaa tgttctactg cgataggtct accggttctc
     1921 cttcgaacgt accggtcaca gatgtggttc ctgtattgaa atcatacgag taattgagct
     1981 ctccggtttt atctccgctg atattgactg ccatgctgaa aacgccggcg ttgtaatcaa
     2041 aggtgcctgt gccgttgacg gtaacgcccg gcatcatttg gcaattctca taggtatggt
     2101 cttcgccttt ttcactgggt gcaaaggtgt aggtgccgcc ataagtacag ccaatagatt
     2161 tttcttcaga ccaatcgttg agatacactt cgggtaaata atagaagttc tcatcgagat
     2221 agatcatcat atccaatgcg tctgcaaagt catttgcact tgcgggtaag ttgggagagt
     2281 agtttgcgta cacggggttc tcccagtctg tacattggat ctcgcgttgg tctggaactt
     2341 tcccgtctac taaaaggtcg gtcacatatt ggtctgggca ggcataacca tacccgaaga
     2401 tggagtgaac gccgccttcc acataaatgt gatagccatc tgctaagttc tcagccacaa
     2461 acttaccttc ttcgaacgga gtagctgggt cgagagttgc gttcaaaacc aaaacaggca
     2521 cgccatctaa cttcagcggc tcacgtgtaa cttcttcctt gggcgaacta ggccagaagg
     2581 cacagtcgat gccggtgtag accgagccgt ccaaacgtgg aacggttccg ttcgacgcct
     2641 gacccgcttc aatcgattgt tgaatgcgtt cctcttgggt gccggtgaag aaggtatcgt
     2701 cagtacatag cacgctgagg aacatcgtat cggagaaagt tgggtcgccg atgtattcaa
     2761 aggtcgccgg gtcaatggtc gcattttcgt acatcaagcg caacatgggg atgatgtcgc
     2821 cttggtttgc ggcagccaat gctcttaaga aaatcatacg ggttgtaaga gagtacatct
     2881 gatacgccgt ggtgaattca agagcattga aagtgaacac accttttact ttttcaccgt
     2941 ttggtaacgg atattcatat tcaatcgggt cttttgagac ttgagctgca aacttatcgt
     3001 atacagccag cgcattctcg ccgcccatat ctgcatgaca aacttcgtct tcatcacagg
     3061 cattcaagac cgctaccaat actttgtcga aggctttctc ttgtgcaagt gaaccctgca
     3121 ctccatccaa tgtcaagtta atggtgccat ccagaatcat gccagccaca cggtctgggt
     3181 gttcggtggc ataggtttga gcgactgccg tgccatagct tacgccatag atccaaagct
     3241 tttcatcacc caacaaagca cggaatgaat caatatcttc cgctacctgg tcagtgccat
     3301 aaaaagctaa cttggcaggg tcaatgccca tttctgcgat acagttttga gcataggcac
     3361 gcgaatcatc aatggcggtt ttctgttctt cgggggtatc caaaccttcc aacccggcag
     3421 tatcgggcgt ggtgaggtac tcaacaaaat ctgcggcata
//
LOCUS       DAOREF010000238         3459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17298_length_3459_cov_3.273502, whole genome shotgun sequence.
ACCESSION   DAOREF010000238 DAOREF010000000
VERSION     DAOREF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17298_length_3459_cov_3.273502"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKI33_06140"
     CDS             <1..703
                     /locus_tag="PKI33_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNM36620.1"
                     /translation="HRAGEVAAELAVNYISMHVAESNARKPIKILENAIHDASQAIAT
                     HSAGKAEEQGMGSTCACVWIVENKLYTAYVGDSRIYLLRGKYIQQITIDHTWVQEAYE
                     KGIITADQIRDHPNVHVIRRHLGGIRLPEVDFRLRLDNNETEEESLNNQGFHLQPGDT
                     ILICTDGLTDLVWDDEIKDIIRGKRDLKTAAETLVNLANERGGHDNITVVLLAMPRVE
                     ETLPKKSILGWLTGE"
     gene            complement(764..1372)
                     /locus_tag="PKI33_06145"
     CDS             complement(764..1372)
                     /locus_tag="PKI33_06145"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNM36621.1"
                     /translation="MNILFPLLGYIFGSLTFSIWITRYVKQVDVRDSGSGHATTTNTI
                     RQAGFGWGALVLILDIAKGFLPTYLATKYSGETWVIALTATAAVIGHCWPVFAQFRGG
                     MGLATAGGSVLAVNPLAFVICLGLLILLVLVVRHSARASVFAGILFAPVLWLLNIRDV
                     AFWVAVGAGAVIAVRFLIDWNRKYRELWLDREKKSEKVEKAD"
     gene            complement(1438..2910)
                     /locus_tag="PKI33_06150"
     CDS             complement(1438..2910)
                     /locus_tag="PKI33_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM36622.1"
                     /translation="MNQPKLILVTGATGYVGGRLVPKLLEAGYRVRCLVRDVSRLQGR
                     AWSKKVEVVAGDALNLDELASAMKDVSVAYYLMHGKQGGKDNAERDLKAARNFAQAAE
                     DEGIEQIIYLGELVDPTANLSPYLRSRHETGFVLRHGKVPVTEIRAGMIIGSGSALFE
                     MIRYLTEREPVLTCPAWFFSQAQPIAIRDVLSYLVDTLKTPDVIGRVIEVGGPTRLTY
                     ADMLLGYAKERNLRRWLIRTPFYAPRLSAYWVHMLTPIHWRVVAPLIEGLSAELIVRD
                     ETAKKLFPHIQPIDFQTAVHLALGRVQRDNVETSWADALVTEAGDVQPYVFTVEDGLF
                     IERRQVVLELSPETVFRSYTGIGGARGWMYMDWAWAIRGWMDKAIGGVGLRRGRRHPD
                     EINTGEALDFWRVELVEKFRIMRLRAEMIVPGKAWLQFESTPLEEEKGKTIFTVTAYF
                     EPYGVPGFLYWYSMWPFHKFIFNGLARRLASRARVLGHAY"
     gene            2970..>3459
                     /locus_tag="PKI33_06155"
     CDS             2970..>3459
                     /locus_tag="PKI33_06155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36623.1"
                     /translation="MNWQLIPEPELSPTLEADIQQMLIASFPDYTDFFTANSYWGTKP
                     EFRLIALENKQPIAHLEFGYRQISIGDMPVNIAGIGAVAIHPHHQGKKLGKLMFSHLR
                     EYLLRHSKVDFGFIGCHEEVAGFYAASGFHRVDQSVYNLNPDSQEWETFHGPTLVMPI
                     HKS"
BASE COUNT          950 a          982 c          758 g          769 t
ORIGIN      
        1 acaccgcgcg ggtgaagtcg ccgcggaatt ggcagttaac tacatcagca tgcatgttgc
       61 tgagagcaac gcccgcaaac ccatcaaaat cctcgaaaat gccatacatg atgccagcca
      121 ggcaattgcc acccattctg ctggcaaagc agaagaacaa ggcatgggct ccacttgtgc
      181 ctgtgtgtgg attgttgaga acaaacttta cactgcctac gtcggcgatt cacgcattta
      241 tcttttgcgc ggaaaatata ttcagcaaat caccatcgac catacttggg tgcaggaagc
      301 ctatgaaaaa ggcatcatca ctgctgacca aatccgcgat caccccaatg tgcatgtcat
      361 tcgccgccac cttggcggca ttcgcctgcc cgaagtagac ttccgcctgc gcctcgataa
      421 caatgaaacc gaagaagagt cgcttaacaa tcaaggcttt catcttcaac ccggtgacac
      481 cattctgatt tgtaccgacg ggctcaccga ccttgtatgg gatgatgaaa tcaaagatat
      541 tatccgcggc aagcgcgacc taaaaaccgc agcagagacc ttggtcaacc tcgctaacga
      601 acgtggcggg cacgacaata tcacggtcgt cttactggca atgccccgcg tagaagagac
      661 tttgcctaaa aagagcattc tcggctggct gactggagaa taaaaaagga cggtaatacc
      721 gtcctttttt tacgcttctt tcttagccga tcatgggtct gtcttaatca gccttttcta
      781 ccttttcgct tttcttctcc cgatccaacc acaactcacg atacttacga ttccaatcaa
      841 tcaaaaagcg gacggctatc accgcccccg cccccactgc cacccaaaat gccacgtctc
      901 gaatattgag caaccacaaa accggtgcaa acagaatccc agcaaagaca ctcgcccgcg
      961 ctgaatgacg aaccaccaag acaagaagga tcaataatcc caagcaaatc acaaacgcca
     1021 gcggattcac cgcaaggaca cttccccctg ccgtcgccag ccccatccca ccgcgaaatt
     1081 gcgcaaagac gggccaacag tgaccgatca cagcggcggt ggcagttagg gcaatgaccc
     1141 acgtctcgcc agaatattta gtagcaagat aagttggcaa gaaccctttg gcaatatcaa
     1201 ggatcaacac caacgctccc caaccaaagc ccgcctgtcg aatcgtattc gtcgttgtgg
     1261 cgtgaccaga acccgagtca cgcacgtcca cctgtttgac ataacgcgta atccaaatgg
     1321 aaaacgtcaa agagccaaag atataaccga ggagagggaa aaggatattc atgcaatcta
     1381 taaccccaaa ctataaaaaa tgctctgtgg agagcgtgcc tctcacagag cattatctta
     1441 gtaagcgtgc cccaaaaccc ttgcccttga agccagccta cgcgccagcc cattgaaaat
     1501 aaatttatgg aagggccaca tcgaatacca atacaaaaag ccaggcacac catatggctc
     1561 aaaataagcc gtgaccgtga atatggtctt ccctttttct tcttccagcg gcgtagactc
     1621 gaattgcagc caggctttac cgggcacgat catctcagca cgcaagcgca tgatgcggaa
     1681 tttttccacc aactccacac gccagaaatc cagcgcttca ccggtgttga tttcatcggg
     1741 atgacgtcgt ccacggcgca agcctacacc accaatggct ttatccatcc acccgcggat
     1801 cgcccacgcc caatccatat acatccaacc gcgcgcgcca ccaatgcccg tatatgaacg
     1861 aaacactgtt tcaggagaaa gctctaatac cacttgtcga cgttcaatga acaagccatc
     1921 ttctacagta aacacatacg gttggacatc ccccgcttcc gtgacgagtg catctgccca
     1981 actggtttcc acattgtcgc gttgaacacg cccaagcgcg agatgaacag ccgtttgaaa
     2041 atcaatcggt tgaatatgcg ggaataattt cttcgccgtc tcatcgcgga cgatcaactc
     2101 tgcgcttaac ccttcaataa gcggtgcgac cacacgccag tgaatcggtg tgagcatatg
     2161 cacccaataa gcagataacc ttggggcgta gaagggagtg cgaatcagcc aacggcggag
     2221 attccgctct ttggcatatc ccagcaacat gtccgcgtag gtgaggcgcg tgggtccgcc
     2281 cacttcgatg actctgccaa tgacatctgg cgtcttgagc gtatccacaa gataggagag
     2341 cacatctcga atggcaatcg gttgtgcttg agagaagaac caagctgggc aggtcaacac
     2401 tggttcacgc tcggtgagat aacgaatcat ttcaaagagc gccgaccccg agccgatgat
     2461 cataccagcg cgaatttctg tcacaggcac tttgccatgc cgcaacacaa accccgtttc
     2521 atggcgcgac cgcaagtacg gcgaaagatt cgctgtgggg tctaccaact caccgagata
     2581 aatgatttgc tcaatgcctt catcttcagc ggcttgcgca aaatttcgtg cggctttcag
     2641 gtcgcgttcg gcattgtctt tgccgccctg cttgccatgc atcagataat acgcaaccga
     2701 cacatctttc atcgcggagg caagttcatc cagattaagg gcatcgcctg ccaccacttc
     2761 tacttttttc gaccaggcac gcccttgtaa acgagacaca tctcgcacaa gacaacgcac
     2821 gcgatacccg gcttcaagaa gtttaggaac aagacgcccg cccacataac cggttgcacc
     2881 ggtgacaagg atcaattttg gttggttcat gatgctggat tataactttt tgagagataa
     2941 aagggacagg ttttataatt cgcctctaca tgaactggca actgattccc gaacctgagc
     3001 tttcccccac tctagaagcg gatatccaac agatgttaat cgcttccttc ccagattata
     3061 cggacttctt cactgccaac tcctactggg gcacaaagcc cgaatttcgg ctgatcgcgc
     3121 tggaaaacaa gcaaccgatt gcccatttag agtttgggta tcgccaaatc tctattggag
     3181 atatgcctgt gaacattgca ggcatcggag cggtagcaat tcatccacat caccaaggca
     3241 aaaaacttgg caagttgatg ttttctcatt tgcgcgaata tcttttgaga cactccaaag
     3301 ttgattttgg ctttattgga tgtcatgaag aggttgctgg tttttatgct gcttcaggct
     3361 ttcaccgtgt agatcaaagt gtttataacc tcaacccaga ttctcaagaa tgggaaacct
     3421 ttcatggtcc aactctggtg atgcccatcc acaaatcca
//
LOCUS       DAOREF010000239         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17336_length_3454_cov_3.781701, whole genome shotgun sequence.
ACCESSION   DAOREF010000239 DAOREF010000000
VERSION     DAOREF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17336_length_3454_cov_3.781701"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..645
                     /locus_tag="PKI33_06160"
     CDS             109..645
                     /locus_tag="PKI33_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36624.1"
                     /translation="MFFGKKETTEKKKNPIRLTILTDEYIIDALDDPDLSILQAVHDS
                     EIGDASGGVIILKDATLHSLRHASAPARTFSEWRLTSLVNMIAILSDDPVAQQVIAEG
                     WEDYQTPFKAFIYAGVYIIEGKMFSEDDTPPDFRIRTFAPLEDATITYALEQKEKTIR
                     GRWGLVNSVLMHGFSIEK"
     gene            675..2075
                     /locus_tag="PKI33_06165"
     CDS             675..2075
                     /locus_tag="PKI33_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36625.1"
                     /translation="MRKIFFVISIFVLTALACQSLTSTPLPSEPLTDSSTQAVSAPQS
                     TALPIGTSTPTSLPKGVIFVDTLQQEVYPFEENGKCSLAEAVIAANTGEPKDTCPAGV
                     PGESIISLMPGEYHFSQRDQSPPQFDWLLSLVKVGSALPPITFPLVIQGNGATLVRDE
                     GSEPFRFFEVMINASLSLEDMTLQNGDVQDDSGGAIYASNGSVTLNRVRFLNNHAEHG
                     GAVYLTFGALTVQESEFIENHAGFQGGGIHLDSAKSTIQNTKFISNTTDARGGALSAE
                     GVTLVVEDSLFLKNVTQDNRGGALHLEHVNVNIARSQFYQNQSAWIGAAIYINNPVMS
                     GTSEDEGDPLEQLDDTPMYVQMATLIPGYEATLQAHPSGVFQDFHEDMQVHQNCFANN
                     IILDTYEMNFSSAILARDVNAEDNYYGTASGPSGSGPGTGDGVGRSVKFAPFLTEPPA
                     YCDPELSQQVKENHNQ"
     gene            2085..2345
                     /locus_tag="PKI33_06170"
     CDS             2085..2345
                     /locus_tag="PKI33_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36626.1"
                     /translation="MAKLIPASERILRARKLIEQARALPVPTEGLGKSDFSYIANVRD
                     VLRQAKDMVKFIPQTAGVSLEMKEEVKKIYAEVEQAEKEILS"
     gene            2434..2691
                     /locus_tag="PKI33_06175"
     CDS             2434..2691
                     /locus_tag="PKI33_06175"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNM36627.1"
                     /translation="MTDKLDALLEQVHAELQKVDEVDADSLKLLQELQQDVQGLLDKT
                     EMETPTVLARMQKAVQQFEVDHPAFTALISELSTVLSNAGI"
     gene            2854..3360
                     /locus_tag="PKI33_06180"
     CDS             2854..3360
                     /locus_tag="PKI33_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36628.1"
                     /translation="MKKTLVIVALVVTALAILGVSAVFAQDVTPPYAGHGPMMNGEGP
                     LHTFMVIEFAKKLDLNVNDINTRLDAGESMYDIALSTGLTAEEFPAVMTEVRTNAVNA
                     AVAANVITQEQADWMLSHGYGQGGMHGNGTGTCDGTGPQGQAGYGHGMMNGGGMMGGR
                     WQNQQTNP"
BASE COUNT          886 a          837 c          850 g          881 t
ORIGIN      
        1 gactttatta tacgagttgc ttccacattc tctatccccc agataggcga ttggggaaat
       61 ccccgctcct tcatataatt gtctgcgtct atatggaagg agagtaccat gttttttggt
      121 aaaaaagaaa ccactgaaaa gaaaaagaat cccattcgtc taaccatcct cacagatgaa
      181 tatattattg atgccttgga tgacccagac ctatccattt tgcaggcagt ccacgactca
      241 gaaatcgggg atgcatccgg cggcgtaatt atcttgaaag acgcgaccct acattcgctt
      301 cggcacgcgt cggctccggc tcgcaccttt tccgaatggc ggttgacctc tttggtaaac
      361 atgatcgcca ttctttcgga tgatcctgtc gcacagcagg tgatcgcaga aggctgggaa
      421 gattatcaga cgcctttcaa ggcttttatc tacgcagggg tctacatcat tgaaggcaag
      481 atgttttcag aagacgatac tccgcccgat tttaggattc gcacctttgc cccgctcgaa
      541 gacgccacaa tcacctacgc attagaacaa aaagaaaaga ccattcgcgg gcgctgggga
      601 ttggtcaact cggttctcat gcatggcttt agtattgaaa aataaatttt atcgtttctg
      661 ttttggagta tgttgtgaga aagatttttt ttgtaatttc gattttcgtt ttaacagcgt
      721 tagcttgcca gtccctgacg agtaccccgc ttccttctga gccgctgact gattcatcca
      781 cccaggctgt ttctgccccg caatcgacgg ctctgcccat cggcacgtcc acgccaactt
      841 ctctgcccaa aggggttatc ttcgttgata cattgcagca agaagtgtat cccttcgaag
      901 agaatggtaa atgttcactg gcagaggctg tcattgctgc caacacaggc gaacccaaag
      961 acacttgtcc tgctggtgtg ccgggtgaat ccattatcag cctcatgccc ggagaatatc
     1021 atttcagtca acgtgaccaa agtccgccgc aatttgattg gttgctaagc ttggtcaagg
     1081 ttggcagcgc cttgccgccg attactttcc cattggttat tcaaggtaac ggcgcgaccc
     1141 ttgtccgcga tgaaggctcg gaacccttcc gcttctttga agtaatgatc aacgccagcc
     1201 tcagccttga ggatatgact ttgcaaaatg gcgacgtaca agacgactcg ggcggtgcaa
     1261 tctacgcctc aaatgggtct gtcaccttga accgcgtccg ttttttgaac aatcatgctg
     1321 agcatggcgg ggctgtctat ttaacctttg gggcactgac cgttcaagag agcgagttta
     1381 ttgagaatca tgctggcttt caaggtggcg gtattcacct cgactctgcc aagtccacga
     1441 ttcagaatac gaaattcatc agcaacacca ccgacgcgcg cggtggtgcg ctttctgccg
     1501 agggggttac cttggtggtt gaagacagtt tgttcttgaa aaatgtaacc caggataatc
     1561 gtggcggcgc tttgcatttg gaacatgtca acgtcaatat agcgcgcagt cagttttacc
     1621 agaaccagtc tgcatggatt ggagccgcga tctatatcaa caacccggtg atgagcggta
     1681 ccagcgaaga tgaaggggat ccgctcgaac agttggatga tacgcctatg tatgttcaaa
     1741 tggcgactct tattcctggc tatgaagcga ccttgcaggc tcaccccagt ggggtgtttc
     1801 aagactttca tgaagatatg caggtccatc agaattgttt tgccaataat attattttgg
     1861 atacctatga gatgaacttc tcaagcgcca tccttgcgcg tgacgtcaat gccgaggata
     1921 attactacgg caccgctagc ggaccctccg gcagtggtcc aggcaccgga gatggggtag
     1981 gtcggtctgt gaaatttgca cccttcctta ccgaaccgcc tgcctattgc gaccctgagt
     2041 tgtcacaaca agtgaaagaa aatcataatc aataggattt catcatggca aaacttatcc
     2101 ctgcatctga acgcatcttg cgcgcccgaa aactgatcga acaagcccgg gctttacccg
     2161 tcccgacaga aggtcttggg aaaagcgatt tttcctatat cgccaatgtc cgcgacgttt
     2221 tgcgtcaggc aaaggatatg gtcaagttca ttccacagac ggcgggtgtt tcgctcgaaa
     2281 tgaaggaaga ggtcaagaag atctacgctg aagtggaaca ggcagagaag gaaatattgt
     2341 cataatcaag gttttatgac gttattcact ggaagcagcc ttgggttggt tgtacaatga
     2401 aagtgaacct gatcctcgaa aggagccaac cccatgaccg acaaactcga cgcacttctg
     2461 gaacaagtcc atgccgagct ccaaaaagtg gacgaagtgg atgccgacag cctcaaactt
     2521 ttacaagaac tgcaacagga tgtgcaagga ttgcttgaca agaccgagat ggagacgcca
     2581 accgtgcttg cccgaatgca aaaggcggtg cagcaatttg aagtagatca ccctgctttt
     2641 acggctttga tctctgaact atccacagta ttaagcaacg cgggaatttg aaagagcccc
     2701 gagtccgcca tgaaggaatt ttccttcatg gcggactttt ttatttttga tgaacgggat
     2761 tcttcatctg acctctacct tttctttaca gaacctttac atgcgggctg tacactttgg
     2821 cttgtaagtt cgaaaacgaa aaggagaaat aaaatgaaaa agacacttgt gatcgttgcg
     2881 ttggtagtga cagcgttagc aattttggga gtaagtgcgg tttttgctca ggatgtcacc
     2941 ccgccgtatg ccgggcatgg acctatgatg aatggggaag gtccgcttca caccttcatg
     3001 gtgattgaat ttgccaaaaa acttgacttg aacgtgaacg acatcaacac tcgcctggat
     3061 gctggtgaaa gcatgtacga tattgccctc tcaactgggc tgaccgctga agaattccca
     3121 gctgtgatga ctgaagttcg taccaacgct gtcaacgcag cagttgctgc aaatgtgatc
     3181 acccaggagc aggcagattg gatgctgtct catggatatg gtcagggcgg aatgcacggc
     3241 aacgggactg gcacttgtga cggaactggt ccacaagggc aggctggata cggtcacggc
     3301 atgatgaatg gcggcggcat gatgggcgga cgctggcaga atcaacagac caatccgtaa
     3361 tcactgcaaa caaaaagaga cggtgcaagc cgtctctttt tgtttactct tttccttcta
     3421 tcaaatactt tttcacatct ccgcccgtcc agat
//
LOCUS       DAOREF010000240         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17349_length_3452_cov_4.176921, whole genome shotgun sequence.
ACCESSION   DAOREF010000240 DAOREF010000000
VERSION     DAOREF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17349_length_3452_cov_4.176921"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKI33_06185"
     CDS             complement(<1..194)
                     /locus_tag="PKI33_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36629.1"
                     /translation="MDGFLGNQLGTYKVQTPVYEGPLDLLLSLIERAELDITTVSLAS
                     VTDQYLEHLRNVDQQKPDEIS"
     gene            230..709
                     /gene="moaC"
                     /locus_tag="PKI33_06190"
     CDS             230..709
                     /gene="moaC"
                     /locus_tag="PKI33_06190"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNM36630.1"
                     /translation="MSKLTHLDEHGRAKMVDVSHKPDTTRTAVARGEVHMTKATFDLI
                     RAGQIKKGDVLTVAQIAGITASKRTSDLIPLCHPLPLSKVDVDLELDESLPGIVITAT
                     AKVTGKTGVEMEALTAVSVAALTVYDMAKAAEKTMHIQNIRLLEKHGGMSGDVINEP"
     gene            720..1088
                     /locus_tag="PKI33_06195"
     CDS             720..1088
                     /locus_tag="PKI33_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNM36631.1"
                     /translation="MNYTSINLKEKLSKFSEHWSPKIIAQLNDYHLKLAKVQGEFVWH
                     DHPDTDEVFIVVQGHLDILFRDGKVSLDEGEMFVVPKGVEHKPVAEKECHILLIEPAG
                     LVNTGSAKDSELTAPNDVWI"
     gene            1090..1338
                     /gene="moaD"
                     /locus_tag="PKI33_06200"
     CDS             1090..1338
                     /gene="moaD"
                     /locus_tag="PKI33_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNM36632.1"
                     /translation="MNHIKILFFATIRDRAGTKSIELDIPADATILQLKEKLSADYPN
                     LKESLKSVLVTINREYVFDEAVVPTNAEIALFPPVSGG"
     gene            1348..1815
                     /locus_tag="PKI33_06205"
     CDS             1348..1815
                     /locus_tag="PKI33_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNM36633.1"
                     /translation="MQPSNHPTIYSITESEIDLNDLLAKITLTSTGAAAIFTGMVRGV
                     TTRGDAHETEYLEYESYVPMAEAKMQQVADEIREKWPVVEGIAIVQRIGKLYPKTPTV
                     LIACTAAHRDTGVFDAARYGIDRLKEIVPVWKKEVGPNGEEWVEGDYVPKPGE"
     gene            1825..2379
                     /locus_tag="PKI33_06210"
     CDS             1825..2379
                     /locus_tag="PKI33_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36634.1"
                     /translation="MHTQRLILRNFELADLDAYVGYRNDPEVGKYQGWVIPYSKEKGE
                     EFIREVQDMHAPKQGEWMQIAIALKETNVLIGDIGCVLRQEDARQAIIGYRIASPHWH
                     KGYAFEAVQAWLDFLFEDLNLHRVIADCDTENTASYKLLEKLGFRREAHFIESYLENG
                     GYTNEYHYGILQREWRAKAAFRNC"
     gene            2410..2988
                     /locus_tag="PKI33_06215"
     CDS             2410..2988
                     /locus_tag="PKI33_06215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36635.1"
                     /translation="MVTIRKAVLKDASAIATVHVAAWKEAYRGIVADEVLNNLSIQRR
                     TEQWTNSLSDETHPYHRAFVAEMNSQVVGFSNFGAPQLKDLGFDGELFAIYILKAAHE
                     IGIGRSLVQAVVNGLREIGSKSMMVWVLKDNPARGFYERLGGVYLYEKPIQIGADELM
                     EVAYGWRDLSIFPVYTHVLEGYQARAGDEALP"
     gene            3072..>3452
                     /locus_tag="PKI33_06220"
     CDS             3072..>3452
                     /locus_tag="PKI33_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNM36636.1"
                     /translation="MQERIVITGMGTVNPLGLNAQESWTNAINGVSGVAPITQFDSQE
                     AKLQVHIAAEVKNFKAENFMDAREARRRDRFEQFAIAASKEALSSSGLQVTEKNAGRI
                     GIIISSAIGGLYSLQEATLTNYKEG"
BASE COUNT          931 a          701 c          885 g          935 t
ORIGIN      
        1 gaaatttcgt ctggcttttg ttgatctaca ttgcggaggt gttcaagata ttggtctgtc
       61 accgaagcga gtgaaacagt tgtaatatcc aattcggcac gttcgatcag gcttaacaaa
      121 agatcaagcg gaccctcata cactggggtc tgaaccttgt acgtgccaag ttgattgccg
      181 agaaatccgt ccataagccc gcgaattata ccagtgtata atcttcgcta tgtccaagct
      241 aacgcattta gatgaacatg gccgtgccaa aatggtggat gtaagccaca agccagatac
      301 gacccgaact gccgttgcgc gcggtgaggt tcatatgacc aaggcgacat ttgacttaat
      361 ccgcgccggg cagatcaaaa aaggcgatgt gttgaccgtg gcgcaaattg ccggcatcac
      421 cgcctccaaa cggacctcag acctgatacc gttatgtcat cctcttccac tctccaaagt
      481 ggatgtcgac cttgagttgg atgaatccct tcctggtatt gtcatcactg ccaccgccaa
      541 agtgaccggc aaaaccggcg ttgagatgga agccctaacg gctgtttccg tcgctgcgtt
      601 gaccgtctac gatatggcaa aagctgccga gaaaaccatg cacatccaaa atattcggtt
      661 gttggaaaaa catggtggta tgagcggcga tgtgattaat gaaccgtgat gggtgaacca
      721 tgaattacac ctctattaat ctaaaagaga aactctcgaa gttcagcgaa cattggtcgc
      781 cgaagattat tgctcaactt aatgactatc atctgaagct agccaaagtt caaggtgaat
      841 ttgtctggca tgaccatcct gatacagacg aagtctttat tgtggttcaa ggtcatcttg
      901 atattctgtt tcgtgatggc aaagtttcac tggatgaggg tgagatgttt gttgtgccca
      961 aaggcgtgga gcataagcct gttgctgaaa aagaatgtca tattttattg attgaaccag
     1021 ctgggctggt aaatactggt agcgcaaaag actcggagtt aaccgctcca aatgatgtat
     1081 ggatataaaa tgaatcacat aaaaatctta ttttttgcca ctatccgtga ccgtgcaggg
     1141 acaaaatcta ttgagttgga tattcctgca gatgcaacca tcttgcagtt aaaggagaag
     1201 ttgagcgcag attaccctaa cttgaaagag tcgcttaagt ctgttttggt cactataaac
     1261 cgcgaatatg tttttgatga agcggtcgtc cctacaaatg ctgaaatcgc ccttttccct
     1321 cccgtatctg gaggataata ttcaactatg caaccttcaa accacccaac catttattcc
     1381 attaccgagt ctgaaattga cttgaacgat ttacttgcca agatcacgct tacatccaca
     1441 ggagcagcgg cgattttcac gggcatggta cgtggcgtca caacccgtgg cgatgctcac
     1501 gagacggagt atctcgaata tgagtcttat gtccctatgg cagaagccaa gatgcagcaa
     1561 gtagcagatg aaatccgcga gaaatggccc gtagtggagg gtattgccat tgttcagcgc
     1621 attggcaaac tctacccgaa aacgccgact gttcttattg cttgtactgc tgcccatcgt
     1681 gatacaggcg tttttgatgc tgcccgttat ggtattgacc gcctcaagga aattgtgcct
     1741 gtttggaaga aagaagtcgg acccaatgga gaggaatggg tcgaaggcga ttacgttccc
     1801 aaacctggag aataagtttt gtttttgcac acccaacgat taatcctgcg gaattttgag
     1861 cttgccgacc ttgatgccta tgtaggctat cgaaacgacc cagaagtagg caaatatcaa
     1921 ggttgggtca ttccttattc aaaagagaaa ggggaggagt ttattcgaga agtgcaagat
     1981 atgcatgctc ccaagcaagg tgagtggatg cagatcgcga ttgctttgaa agagactaat
     2041 gtattaatag gcgatatcgg ttgcgtattg aggcaagaag atgcccggca ggcaattatt
     2101 ggttatcgta ttgcttcccc tcattggcat aaaggatatg cttttgaagc tgttcaagcc
     2161 tggttggatt ttttgtttga agaccttaat ttacatcgtg tcattgcaga ttgtgacaca
     2221 gaaaacacag cctcatataa attgcttgaa aagctaggtt ttcggcgcga agctcatttt
     2281 attgaaagct atttagagaa tgggggctat accaatgaat atcattacgg catccttcaa
     2341 cgtgaatggc gtgccaaggc tgcatttcgt aactgttgat ttgttctgag ccttaagaag
     2401 ggagcaaaga tggtcactat tcgtaaggct gttttgaaag atgcttctgc gattgctact
     2461 gtccatgtgg cggcgtggaa ggaggcgtac cgtggcattg tggcggatga ggtgttgaac
     2521 aatctgtcca ttcagcggcg aaccgagcag tggaccaatt ctctttctga tgaaacacac
     2581 ccatatcacc gcgcatttgt ggcggagatg aatagtcagg tcgtgggttt ttcaaatttt
     2641 ggcgcacctc aattaaaaga cttgggcttt gatggcgagt tgtttgcaat ttatattttg
     2701 aaagcggctc acgaaatagg aataggaagg tcattggtgc aggctgttgt caatgggttg
     2761 cgggaaatag gtagtaaatc catgatggtg tgggtgttga aagataaccc agcgcgtgga
     2821 ttttacgagc gtttgggtgg tgtatatctc tatgaaaagc ctattcagat cggtgcagac
     2881 gagctgatgg aagtggcgta tgggtggcga gatttgagca tattccccgt gtatacccat
     2941 gttttagagg gctatcaggc aagggcgggg gatgaagcgt tgccttaatt ttatcgatgg
     3001 agggcgtcac gattctgaga atcctgtgtt gttaatatcg ttatattcat ttttttatcg
     3061 tcggaggcga gatgcaggaa cgtatcgtga ttacaggcat ggggactgta aacccacttg
     3121 gattgaatgc gcaggaatca tggacgaatg cgattaatgg ggtgtctggt gtggcgccaa
     3181 tcacacagtt cgactcacag gaagcgaagt tgcaagtaca tattgcggca gaagtgaaaa
     3241 acttcaaagc agaaaatttt atggatgcgc gcgaagctcg acgccgcgac cgctttgaac
     3301 agtttgccat tgccgcttca aaagaagctc tgtcgagttc tgggttgcag gttaccgaga
     3361 aaaatgctgg gcgtattggc attattattt catctgcaat cggtggtttg tattcattgc
     3421 aggaagccac tctcacaaat tacaaagaag gt
//
LOCUS       DAOREF010000241         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17469_length_3438_cov_3.581732, whole genome shotgun sequence.
ACCESSION   DAOREF010000241 DAOREF010000000
VERSION     DAOREF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17469_length_3438_cov_3.581732"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1184)
                     /locus_tag="PKI33_06225"
     CDS             complement(66..1184)
                     /locus_tag="PKI33_06225"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNM36637.1"
                     /translation="MPLLVDAHADIAYNMLKYDRDYTRTVSETRALEAGSHTVQDNGD
                     TLISWHEYQRGKIAIIFSTLFAAPIRFRSYEGEKQIYKTFDEAHKLYSAQLDVYHRMQ
                     DDMPDTFRILASKRDLDLHLTQWSESTPDASAHAAVSSGSRPLGMVVLMEGAEGIREL
                     SELEMWHNRGVRLIGPAWVGTRYCGGWKEPGPLTDDGRKLLAAMADFNFTLDLSHMDE
                     RAAIEALDIYEGPIVGTHANCAALMPNSNTNRHFSDRIIEGIIERDGVVGVVPFNTYL
                     KVGWSREKGHRREEVPLDVLANHIDHICQIAGDSLHAGIGSDFDGGFGLQSVPPEIET
                     VADLQKLVSLLQARGYSETDTTNILGSNWIARLKRDLP"
     gene            complement(1230..2429)
                     /locus_tag="PKI33_06230"
     CDS             complement(1230..2429)
                     /locus_tag="PKI33_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNM36638.1"
                     /translation="MDQNETLSGTNNAQGDHNNQSMEALLASESLSIDLPKAGEIRKG
                     TIASISQNQILISIGAKSEGVVAGKELEQLTEEERAALKVGQELDVFVIIPEDNNGNV
                     VLSLKRAFEQIAWENVEKMIADETVFDTKIIGFNKGGLIAAIGSLRGFIPSSQISAAR
                     RAQSTGDKPEQRYQKMVGQPISVRIVEVDRERRRLILSERAANTESRSELKERVIGEL
                     EEGKSYTGRVTSLADFGAFVNINGADGLVHLSEISWDHVTHPKEVLEVGQEVNVKVIN
                     IDRDKKRIGLSIRALQDDPWKNRMSKFSVGQLVEGVITRLTKFGAFARLEGDIEGLIH
                     ISELSENRVEHPKEVLHEGDVKTLRIIRIDVDQHRIGLSLRKVDSAAFADKDFKMLTK
                     DFGGDEE"
     gene            2575..>3438
                     /locus_tag="PKI33_06235"
     CDS             2575..>3438
                     /locus_tag="PKI33_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM36639.1"
                     /translation="MPAIFPFTAIVGQDRMKRALILNAVDTRIGGVLIRGERGTAKST
                     ASRSLAALLPHVKVVQDCRFGCDPDKPGTWCTECKERAAAVKNLPFAERATPFINLPV
                     SATEDRVVGTLDIEKAIQKGERHFEPGVLANANRGLLYIDEVNLLDDHVVDILLDSAA
                     MGVNTVEREGISFAHPARFILVGTMNPEEGDLRPQLLDRFALSVEIVGIREARERMTI
                     MERNISYERDPEAFRKQWQPKEDELSQQIAKARELVDQVKYTSRDLLSIAALTASLNV
                     DGHRADMVILKA"
BASE COUNT          801 a          925 c          879 g          833 t
ORIGIN      
        1 ggtgtaaggg catcgtcgct gccagaatta ttgtcagaag aaggttgagt atcagtcata
       61 agggtttagg gtaaatctcg tttgaggcgg gcgatccagt tgctaccgag aatattggtg
      121 gtgtctgttt cagaatagcc gcgcgcttgc aataaggata ccagtttttg taaatctgcg
      181 acggtctcaa tttctggcgg aaccgattgc aacccaaagc cgccgtcaaa atcggagcca
      241 atgccagcat gcaacgagtc accggcgatc tggcatatgt ggtcaatgtg attagcgagc
      301 acgtccaacg gcacttcttc gcggcggtgt cccttctcgc gtgaccaacc caccttgaga
      361 tacgtattga acggcacgac tcccaccacc ccatcgcgct cgatgatgcc ttcgatgatg
      421 cgatcactga agtgccggtt cgtgttggag ttgggcatga gtgccgcaca gttggcatga
      481 gtgcccacga tgggaccttc ataaatatcc aaagcttcga tggcggctcg ttcgtccatg
      541 tggctgaggt cgagggtgaa gttaaagtct gccatggcag ctaacaactt gcgcccgtca
      601 tcggtcaacg gaccgggttc cttccaaccg ccacaatagc gcgtgcccac ccaggcaggt
      661 ccgatcaggc gcacgccacg gttatgccac atctctaatt cagagagttc gcgaatgcct
      721 tctgcgcctt ccatcagcac caccatgcca agcggtctac tgccgctgga gacggcggca
      781 tgagcagatg catccggcgt ggattcgctc cactgggtca gatgcaggtc caggtcccgt
      841 ttggaggcga ggatgcggaa tgtatccggc atgtcatcct gcatccgatg atagacatcc
      901 aattgagcgc tatagagttt atgcgcttcg tcgaaggttt tataaatctg tttttcgcct
      961 tcataacttc taaagcgaat gggtgccgca aaaagggtcg agaaaataat ggcgatcttg
     1021 ccgcgttgat attcgtgcca actgataaga gtatccccat tatcttgcac agtgtgactg
     1081 cccgcttcga gtgcgcgggt ctctgatacg gtgcgcgtat aatcgcggtc gtacttgagc
     1141 atgttgtagg caatatcggc gtgagcgtca actaagagtg gcataagcat aattgaaaag
     1201 cgcaccttgc ggtgcgctct ttttgttttt tattcttcgt cgccgccgaa gtctttcgtc
     1261 agcatcttga agtctttgtc ggcgaaagcg gcagagtcta ctttgcgcag gctcaagcca
     1321 atgcggtgtt ggtcaacatc gatgcggatg atgcgcaagg tcttgacatc accttcgtga
     1381 agaacttcct tgggatgttc aacgcggttc tcacttaact cagagatgtg gatgagacct
     1441 tcgatatcgc cttcgaggcg ggcaaaagca ccaaacttgg tcaggcgggt aatgacacct
     1501 tccaccaact gacccacgct gaacttggac atgcggttct tccaggggtc atcctgcaag
     1561 gcacgaatgg acaagccgat gcgcttcttg tcgcggtcaa tgttgatgac cttgacgtta
     1621 acttcctgcc ccacttcgag gacttccttg gggtgagtaa catggtccca agaaatttcg
     1681 gagaggtgca caagaccgtc ggcaccgttg atattgacga aggcaccgaa gtcggcgagg
     1741 ctggtcacgc gaccggtgta agacttgcct tcttccaatt caccgatcac acgttcctta
     1801 agttctgaac gagattcggt attggcagca cgctcagaaa ggataagacg gcggcgttcg
     1861 cgatccactt ccacaatgcg gacggagatc ggctgaccga ccatcttctg gtagcgctgt
     1921 tcgggcttat cacccgtaga ctgcgcacgg cgagcggcgc tgatctgcga agagggaatg
     1981 aacccacgca aactgccaat ggcagcgata agaccgccct tgttgaaacc gatgatcttt
     2041 gtatcgaaca cggtttcatc agcaatcatc ttttcgacgt tttcccaggc aatctgttcg
     2101 aacgcacgtt tgagagaaag caccacattc ccgttgttat cttccgggat aatgacaaac
     2161 acgtcaagtt cctgtccgac tttcagcgca gcgcgctctt cttcggtcag ttgctccaat
     2221 tccttgccag caacgacgcc ctcggacttc gccccaatgc tgatcaggat ctggttttgt
     2281 gaaatacttg caattgtccc tttgcggatt tcgcctgctt taggaagatc aatgctcaac
     2341 gactcagatg ctaatagcgc ctccatcgat tgattgttgt gatctccctg cgcattattt
     2401 gtcccgctca gggtttcatt ttgatccatc acttgtgact ccagttaaaa gaaatatgaa
     2461 caagattata caagtgaaac gacaagttga caaccctaat aagaggaagg aacacataat
     2521 ttgcgagtaa gtttccaaaa aaagggcgtt ataatcacgg gctggagtaa acaaatgccc
     2581 gctattttcc cctttacagc tatcgttgga caagaccgca tgaagcgtgc gctcatcctc
     2641 aacgccgtag atacccgtat cggtggcgta ttgatccgtg gtgaacgcgg taccgcgaaa
     2701 tctactgcat cccgttctct ggctgcgctt ctcccccacg tcaaagtcgt gcaagattgc
     2761 cgctttggct gcgacccaga taaacccggc acttggtgca ccgaatgtaa agaacgtgcc
     2821 gctgccgtaa agaatttgcc gttcgccgaa cgcgcaactc ctttcattaa cttgcccgtt
     2881 tctgctaccg aagaccgtgt agttggaacg ctcgacattg aaaaagccat tcaaaaaggc
     2941 gaacgccact ttgaacccgg tgtgcttgcc aatgccaacc gcggcttgct ctacatcgac
     3001 gaagtgaact tgctcgacga tcatgtggtg gatattcttt tggactctgc cgccatgggc
     3061 gtgaacacgg tggagcgtga aggcatttcc tttgctcacc ccgctcgctt cattctcgtt
     3121 gggacgatga atcctgaaga aggcgatctt cgccctcagt tgctggatcg cttcgccctc
     3181 tcggtggaga tcgtaggaat ccgcgaagcc cgcgaacgca tgaccatcat ggaacgcaat
     3241 atttcctacg aacgcgatcc cgaagccttc cgcaagcaat ggcaacccaa agaagatgaa
     3301 ctctctcagc aaattgccaa agcccgcgaa ctcgtagacc aggtcaaata caccagccgc
     3361 gatttgctct ccatcgccgc cctcaccgcc tctctcaacg tggacggtca ccgtgcggat
     3421 atggttatct tgaaagcc
//
LOCUS       DAOREF010000242         3429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17539_length_3429_cov_3.727919, whole genome shotgun sequence.
ACCESSION   DAOREF010000242 DAOREF010000000
VERSION     DAOREF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17539_length_3429_cov_3.727919"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="PKI33_06240"
     CDS             complement(<1..1372)
                     /locus_tag="PKI33_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36640.1"
                     /translation="MMQRLTRQLVIADVLACVIVIAALKIILTGISASVPNTVNNQYF
                     FVIGLVATSIGFSLSKAQRNGVQASAGIAALGILLVWIIGARLPQPLFDLVSAIFNTL
                     KQVIPSIQHDTAVETTAIQSAWAVIASSSVTLWSRLQTWLAGFTSKTVNDALMRSLAW
                     ILLYWVISAWLGWYTNKRNALIAFLPALFLLTALTAYSEKKIDSVLALLAALLLLMGI
                     WHYKSHMALWMKRRLDFSESLPLDVGQAIIAITVAVVLLAALTPSLSWDDLVNLIRRN
                     NTDSVAKMLGVRPPATSNGSGTTQPPSMPREHLLIGGFANSEKVVMTIQTGELPPLPT
                     ELLPSPAPRYYWRSQVYDQYLGTGWGTSTVGGQKISANTPLLPALLNAYRPLNLDVKM
                     IEPEGKLFWSGILFRVDVPFTAQWRLRPPSDLFADQTALLQSDIFYASSNSTTYHVEA
                     YIPSPAI"
     gene            complement(1369..2655)
                     /locus_tag="PKI33_06245"
     CDS             complement(1369..2655)
                     /locus_tag="PKI33_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNM36641.1"
                     /translation="MKSTLRLNAKAFPVIAIIALILQIITPSRVWVTLWIVLGGVWLL
                     CWWWVKGLSRSLEFKRELRFGWAQVGDKLEERFTISNPFILPVIWIAVDDHSTLPDHY
                     ASLVISVDGRSSFQWKLNSQCTHRGVFTLGSTTLETGDPFGIYTLKFEDPSSSTVVVM
                     PPVISLPRFNILASGWAGDGRPSPRALEETTNISHTREMVSGDSLRLVHWKTSARKNK
                     LHVREFDGTQAGNWWILLDLHKDSQVGTGWDSTEEHAVILAASLIVQGLNEEHPVGLA
                     VNGHEPTWHIPRRNEYQSHALLKALAIAKPSEHPLREFIQRNSKSLGQRSSLLIITAS
                     HDPTWVEDLIPLIRRGILPTVFLFDVNTFGAQADSRSLSSTLAELGVPCHIIPKDLVG
                     SQQIRRGHEGEWKWRVTGTGKAVAVQQPIADWRALE"
     gene            complement(2816..>3429)
                     /locus_tag="PKI33_06250"
     CDS             complement(2816..>3429)
                     /locus_tag="PKI33_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNM36642.1"
                     /translation="FIEELMRPLRWVEEIRRSVPAYKFWGGKYPSTVTQFVAGGSDES
                     DLELLNTTQWLMKNVRLKRAYFSAFHPIRDTPLENKTAVDPIREHRLYQASFLLRDYG
                     FDLEDLPFTADENLPLHTDPKLAWAQMNLTHTPVEVNRADRRELMRVPGIGQKGADAI
                     LSARRLNKLRDISALKKLGIIAERAAPYVLLDGKRLASQLALF"
BASE COUNT          908 a          772 c          949 g          800 t
ORIGIN      
        1 aaatcgcggg gcttggaata taggcttcga catgataggt tgtggagttg ctggaggcat
       61 aaaagatatc agactgcaac aaagccgtct ggtcagcgaa caggtctgag ggcgggcgga
      121 ggcgccactg ggcggtgaat ggcacatcca cacgaaagag aatcccgctc caaaataatt
      181 tcccttcggg ctcgatcatt tttacatcca aattaagtgg acgataggca ttcagtaggg
      241 caggcagtaa gggtgtattc gcggatattt tttgcccgcc cacggtgctg gtgccccaac
      301 ctgtgccaag atattgatcg tagacctgac tacgccagta ataacgcgga gcgggtgatg
      361 gcaaaagttc agttggcaat ggcgggagtt cccctgtttg aatcgtcatg acgacctttt
      421 cagagttggc aaagccgcca atgagcagat gttcgcgtgg catggacggt ggttgtgttg
      481 tgccagaacc attggatgtg gcaggagggc gcacccccaa cattttggca accgagtcag
      541 tattgtttct gcgtatcaaa ttaacaaggt catcccatga aagcgatggg gtaagtgcag
      601 ccagtaagac aactgcaact gtgatcgcaa tgatggcttg ccctacatcc aatggaaggc
      661 tttctgaaaa atctaagcgc cttttcatcc ataaagccat atgagatttg tagtgccaaa
      721 ttcccatgag taacagcaaa gctgccaaga gggctaatac agagtctatt ttcttctcac
      781 tatatgctgt gagtgccgtt agcaaaaata aagcaggcag aaaagcgatt aaggcattgc
      841 gtttattcgt ataccaacct agccaagcag agatgaccca atacaacaaa atccaagcca
      901 aactgcgcat aagggcgtcg ttcaccgttt ttgaggtgaa gcctgcaagc caagtttgca
      961 accgagacca taatgtgaca gaagaagaag caataactgc ccaagcggat tgaatagcag
     1021 tcgtttctac tgcagtgtcg tgttgaatcg atgggatgac ttgcttgagt gtgttgaata
     1081 tcgcagaaac aaggtcaaat aaaggttgag ggaggcgcgc gccaatgatc caaacaagga
     1141 ggatgcccaa cgccgcaatc cccgctgaag cttgaacgcc atttcgttga gctttactca
     1201 aactgaagcc gatgctcgta gcgacgagac cgattacaaa aaaatattga ttgtttactg
     1261 tgttgggcac agaggctgaa atacccgtta gtatgatctt gagcgctgcg atgacaatca
     1321 cacatgccaa aacatctgct atgacgagct gacgtgttaa gcgctgcatc attctagcgc
     1381 tctccaatcg gcaatgggct gttgcacggc aacggctttg ccagtgccag tgactcgcca
     1441 tttccattcg ccttcatgac cacgccgaat ttgctgtgac cctaccaagt ctttagggat
     1501 gatatgacat ggcacgccga gctcagccaa cgtggacgat aacgagcggg aatcagcctg
     1561 tgcgccgaag gtatttacat cgaaaagaaa gacggtgggc aatatcccgc ggcgtataag
     1621 tggaatgagg tcctcgaccc atgtagggtc atgcgaggcg gtgatgatta acagactgct
     1681 gcgctgacca agcgatttgc tgttgcgttg aataaattct ctgagtgggt gctctgaagg
     1741 tttggcgatt gccaacgctt tgagcaaagc atgagattga tattcatttc ggcgcggtat
     1801 atgccaggtg ggttcatgcc cattgacggc taacccaacg ggatgctcct catttagacc
     1861 ttgaacaatg agcgacgctg ccagaatgac ggcgtgttct tcggtagagt cccagcctgt
     1921 gccgacttgc gagtctttgt gcaggtcgag aagaatccac cagtttccag cttgggtgcc
     1981 atcaaattcg cggacgtgga gtttgttctt gcgggcactg gtcttccaat gcacgaggcg
     2041 caatgaatct cctgacacca tttcacgagt gtgagagata ttggttgttt cttccaatgc
     2101 acgtggactg ggtcttccat cacctgccca accgctggca aggatgttga agcgtggcag
     2161 agaaatgacc ggtggcatga ctacaacagt agatgaggaa ggatcttcga acttgagggt
     2221 atagatccca aaagggtcgc cggtttcaag tgttgtgcta cccagtgtga aaactccgcg
     2281 atgagtgcat tgcgaattga gcttccattg aaaagaagat cgcccgtcga cgctgatgac
     2341 gagggatgca tagtgatcgg gcagagtgga gtggtcatct acagcaatcc aaatgaccgg
     2401 caaaatgaat ggattgctga tcgtgaagcg ttcttcgagt ttatcgccga cttgagccca
     2461 gccaaagcgc agttcacgtt tgaattcaag tgagcgagaa agacctttaa cccaccacca
     2521 gcaaagaagc caaacgccgc ccaagacaat ccataaagtg acccacactc gtgatggtgt
     2581 aatgatctga agtatgagtg cgataatggc aatgacaggg aatgcctttg cattcaatcg
     2641 caaagtagat ttcaagtgcg ggttatacga tggagtggga aggcgtaggc gttgcgtcgt
     2701 tcttttagga agtaacatag cgacccagac ggcaaaataa agatgcggtt gcagtaaggc
     2761 aaccgtggaa caaactttat atatcgatta tacgacgggg taggggtgta gaactttaaa
     2821 acaaagcgag ctgacttgct aagcgtttgc catctagcaa aacataggga gcggcacgct
     2881 cagcgatgat gccaagcttt ttcagggcag agatatcacg cagtttgttg agcctgcgag
     2941 cggagaggat ggcgtcggca cctttttgac caatgcccgg tacacgcatc aattcgcggc
     3001 ggtcggcgcg gttgacttcg actggagtgt gagtcaagtt catttgcgcc cacgccagtt
     3061 tcgggtcggt gtgaagcgga aggttttcat ccgctgtgaa gggcaagtct tcgaggtcga
     3121 agccatagtc gcgcaataag aatgaggctt gataaaggcg atgctcgcgg atggggtcga
     3181 cggcggtttt gttttcgagg ggtgtatcac gaatgggatg gaaggcagag aaataagcgc
     3241 gtttgaggcg cacgttcttc atcagccatt gagtggtgtt gagcagttca aggtcgcttt
     3301 catcagaccc gcctgctaca aattgcgtca ctgtagaggg atacttgccg ccccaaaatt
     3361 tataagcagg cacactgcga cgaatctctt caacccacct gagcgggcgc atgagttctt
     3421 cgatgaaaa
//
LOCUS       DAOREF010000243         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17545_length_3428_cov_3.836347, whole genome shotgun sequence.
ACCESSION   DAOREF010000243 DAOREF010000000
VERSION     DAOREF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17545_length_3428_cov_3.836347"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKI33_06255"
     CDS             complement(<1..680)
                     /locus_tag="PKI33_06255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNM36643.1"
                     /translation="MIFALTLLLVILAGAGIFALRPIVQKINTVFHIDKTVDYQLKFQ
                     SLQLILATVVLVLTYLLNPQNFQIFFQPGNINANISKIPWLGVTGQETWSEIALTLGF
                     FITVATGIFMFLQLKKAGVDYRYFLFSLLWSIPFSIANSFSEEAIFRMGIVSPLYGIF
                     SVSIIILISGVLFGLPHYFGQPSGIVGVLMAGFLGWLLAMSLVETQGLLIAWAIHFAQ
                     DVVIITSM"
     gene            complement(695..1078)
                     /locus_tag="PKI33_06260"
     CDS             complement(695..1078)
                     /locus_tag="PKI33_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HNM36644.1"
                     /translation="MSRKLLKTFLIILGLIPTITGVLTMMGIHDPLFADLALPHSALL
                     DSELRFFGGLWLGLGLTVLSTVRNLEKHFDLYRVLWIMIFLGGVGRLLSILLTGLPPI
                     PFIAFTVLEIVGAPIFIYWHSQIAE"
     gene            complement(1083..1541)
                     /locus_tag="PKI33_06265"
     CDS             complement(1083..1541)
                     /locus_tag="PKI33_06265"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNM36645.1"
                     /translation="MIYTIAKWIHILCAIVSLGANLTYFPWFMTVPKTRETLVYTLKT
                     IRLLDNWIANPAYILAYITGEVMMRVGGQIHYSTSWMTVAISLYAIISVLGLFIYSPM
                     IKKQQQLAETKGADDADYQRVSRNGIVLGTVIVILTIGITYLMVAKPHLW"
     gene            complement(1630..2187)
                     /locus_tag="PKI33_06270"
     CDS             complement(1630..2187)
                     /locus_tag="PKI33_06270"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNM36646.1"
                     /translation="MPLEHAILAILDFQPMSGYDLKKFFDVSVAHFWSATQSHIYKSL
                     EGLEKKGWAEAQVIPQEGKPNRKEYQITAEGRSELRRWLVTPLSMDPVREAVLIQIFF
                     SHSSSNEEIAALFESRIKEIREQLQVLKTSAQAAINENGNLIGIERARQLWQITLDYG
                     IDYYEFELAWNEKIIKRIRKLPPIS"
     gene            2366..>3428
                     /locus_tag="PKI33_06275"
     CDS             2366..>3428
                     /locus_tag="PKI33_06275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNM36647.1"
                     /translation="MNRSEILAQLKNNPEVSVLIVGAGINGIGTFRDLALNGVDVLLI
                     DRGDFCSGASSASSHMAHGGIRYLENGEFRLVREAVKERNRMIQNAPHIVKPLPTTIP
                     MFKYFSGILNAPLKFMGWLDKPSERGSIIIKLGLMMYDAYTGSQRTVPRHKFYSRTES
                     LTKWNKLNPEIVNTAVYYDGLISNPERLALEIMLDAETENSNARALNYVSLVSGSGKN
                     VILRDELTDATVEIQPKLVINAAGPWIDFANHSLGVTTKFIGGTKGSHLVLDHPELRK
                     AIGDNEFFFENKDGRIVLIFPLHDRVLIGTSDIKIDHPDDARCTDEEVDYFLELTARV
                     FPNIKVTREHVVFAFSGVRP"
BASE COUNT          972 a          746 c          790 g          920 t
ORIGIN      
        1 atcatggagg tgatgatgac cacgtcctgt gcgaagtgga tcgcccatgc gatgagaagt
       61 ccctgtgttt cgacaaggct cattgccaac agccagccga ggaagccagc catcaaaacg
      121 ccgacgattc cactgggttg cccgaagtag tgggggagac caaataacac tccagaaatc
      181 aggataatga tagagacaga gaagattcca tacaacggtg agacgattcc cattctgaag
      241 atggcttctt cagaaaagga attcgctatt gaaaaaggaa tggaccaaag cagactaaac
      301 agaaaataac gatagtccac acccgctttt ttcaattgca agaacatgaa tatgcccgtc
      361 gcaacagtga taaaaaatcc gagggtcagg gcgatctctg accaagtctc ttgacctgtc
      421 acaccgagcc atggaatctt actaatgttt gcattgatat tgccaggctg gaagaaaatt
      481 tggaagtttt gcgggttgag aagatatgtc aacaccaaca cgaccgtagc tagtatcaat
      541 tgcaaagatt gaaacttgag ctgataatca acagttttat ctatgtgaaa tacggtgtta
      601 attttctgca cgatagggcg cagtgcgaag atgcctgcac cagccagaat gacaagaaga
      661 agggtgagag cgaatatcat ttgattctcc aaaattattc agcgatctgg gaatgccagt
      721 agataaagat aggtgcgcct acaatttcga gtactgtaaa cgcaataaat ggtattggcg
      781 gtaaacccgt caacaaaata gagagtaacc gaccaacacc cccgagaaag atcattatcc
      841 aaagcacgcg atacagatcg aaatgctttt cgagatttct cacggtagat aaaactgtca
      901 gtccaagacc tagccaaagt ccgccaaaga agcgcagttc gctatccagc aaggcagagt
      961 ggggcagggc aaggtccgca aataacgggt catgaatccc catcatcgta agaacgcctg
     1021 taatggtggg aattaaccca aggataatga ggaaggtttt taataatttt cgtgacatgg
     1081 atttaccata agtgtggttt tgccaccatc aaataggtga taccaatggt gaggataaca
     1141 atcaccgttc ccaatacaat gccattgcgt gagacgcgct gataatcggc gtcatccgcc
     1201 cctttggttt cagcaagttg ctgttgtttc ttaatcattg gtgaataaat gaaaagcccc
     1261 aacactgaaa taattgcgta caaactaatt gccactgtca tccacgatgt ggagtaatga
     1321 atttgtccac ccacgcgcat catgacctcg ccggtgatgt acgccaaaat ataagcaggg
     1381 ttggcgatcc agttatcgag cagacgaatg gtcttgagag tgtacaccag cgtttcgcgg
     1441 gtctttggta cggtcatgaa ccaggggaag taggtcaggt ttgctccgag tgaaacaatg
     1501 gcgcagagaa tgtgaatcca tttggcgatt gtataaatca tgtcttatct ccttgagttg
     1561 cgctgaggcg cgtttaatag acccattgtg atgtgggtgg tttaataaat ttatttttct
     1621 tatgtcaact tacgaaatcg gtggaagttt tcgtatgcgc tttattattt tttcattcca
     1681 agccagttca aactcgtaat aatcaattcc ataatccaac gtgatttgcc agagctgacg
     1741 cgctcgttca atgccaatca ggtttccatt ttcgttgata gctgcctgtg ctgacgtttt
     1801 taagacctgc aactgttcac gaatttcttt tatcctagat tcgaatagtg ctgcgatctc
     1861 ttcattacta ctagaatgcg aaaagaaaat ttggataaga actgcctcgc gcacaggatc
     1921 catagatagg ggagtaacca gccagcgacg taattcgctt cttccttccg ccgtgatctg
     1981 atactctttt ctgttgggtt tcccttcctg tggaatgacc tgtgcctctg cccaaccctt
     2041 tttttccaat ccttcaagag atttataaat atggctctgt gtcgcagacc aaaagtgtgc
     2101 cacagacaca tcaaagaatt tttttaaatc ataaccagac atgggctgga aatcgagtat
     2161 ggcaagaata gcgtgttcta agggcataat actcctctag ttttatatat ctatatagat
     2221 ataatatcaa atagtcttat ggatgtcaag gttaactaaa ccaatttgtt ttcagtttga
     2281 tcactctgtt atgacgttca atcattcagc ccaaaaaatt agaaatcatc gtacaattaa
     2341 ccccgtattg tgaagggatg ttcaaatgaa tcgttcagaa attctagcgc agctcaaaaa
     2401 taatcctgaa gtttctgtct tgattgtagg ggcggggatt aacgggatcg ggacttttcg
     2461 tgatttagca ttgaacgggg tcgacgtttt acttattgac agaggagatt tctgctctgg
     2521 tgcaagctca gcttcttcgc acatggcaca cggtgggatt cgttatcttg agaacggtga
     2581 gtttcggttg gtgcgtgagg cggtcaaaga acgcaatcgt atgatccaga atgcgccgca
     2641 tattgtcaag ccgctgccga cgaccatccc gatgttcaag tatttttctg gcatcttgaa
     2701 tgcaccgctg aagtttatgg gctggctcga taaaccttct gaacgcggct cgatcattat
     2761 caaactaggg ttgatgatgt acgatgccta tacaggcagt caacgcacag tgccgcgcca
     2821 taaattctat tcccgcaccg aatcccttac caaatggaat aagttaaatc ctgagatcgt
     2881 caacacggcg gtctattatg acggattgat ttctaaccca gaacgcctcg cacttgagat
     2941 catgctggat gctgaaacag aaaattcaaa tgcccgcgct ttgaattacg ttagccttgt
     3001 gagcggatcg ggcaagaacg tcatcctccg tgatgagttg accgatgcaa ccgttgaaat
     3061 ccagccgaag ttggtcatta atgccgccgg tccatggatc gactttgcca accacagcct
     3121 gggtgtgact acgaaattta ttggcggcac gaaaggttca caccttgttc tagatcaccc
     3181 cgaattgcga aaagcgatcg gcgataacga atttttcttt gaaaataaag atgggcgtat
     3241 cgtgttgatc ttcccgttac acgaccgtgt tttgatcggc acctctgata tcaagatcga
     3301 tcaccctgat gatgcccgtt gtaccgatga agaagtggac tacttccttg aactaactgc
     3361 acgtgttttc ccgaatatca aggtgacacg tgagcatgtg gtctttgcat tttcgggtgt
     3421 gcgcccgt
//
LOCUS       DAOREF010000244         3427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17552_length_3427_cov_4.218565, whole genome shotgun sequence.
ACCESSION   DAOREF010000244 DAOREF010000000
VERSION     DAOREF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17552_length_3427_cov_4.218565"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PKI33_06280"
     CDS             <1..886
                     /locus_tag="PKI33_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36648.1"
                     /translation="STLRRGFGTALRLIGAASTLSILGHIQILPTNLTPILMILTSVA
                     AIYGGWTWLRAPDELNGRPYWMIGMASLAILSALSGNATGAIAWGSALVLVGGALFLF
                     SIQDIRLSRALLIGGWALSSLPLSLTASTWASSFNISTIFSLAAQVLLTAGYIRHALR
                     RNGTETLDDQPGWAKVVYPAGILLLLVTQILLGFIGWDGAFKFGNLIFSLLASILALG
                     LVGGSRRFRIFNPVRAHWVTTAGSGVNGLYQWLWSLYRGLAQVAQAITNALEGEGGIM
                     WTLLFLILFISIIVQGIQ"
     gene            887..1510
                     /locus_tag="PKI33_06285"
     CDS             887..1510
                     /locus_tag="PKI33_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36649.1"
                     /translation="MVSDIVSWISVALTLVTATMILVNRDWRISLGALALQYLAAFWL
                     VTRHLPFAMGSVKLITGWMVVAILGMTRLSLTNLDQNEQDAFYPRGAAFRITLMGIVT
                     LVALGSTPRIEEAIPGLGMPVIAGGLLLIGAGVAHLGVTNDLLRVALGLLTVLTGFEI
                     IYAAVESAILVTGLLAVVNLGLGVMGSYLLMAGSIPLISPEEEEELL"
     gene            1507..2904
                     /locus_tag="PKI33_06290"
     CDS             1507..2904
                     /locus_tag="PKI33_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNM36650.1"
                     /translation="MSAPILWIAVPFVLGVLILFVLSERYAAYVGGTISTILAAIAFF
                     IPIDTALLLGNVSLKISPAIEFFGRSFELSTADGRLLTLIYGLAALWFFGTEASGTAH
                     RFVSLGLMITALLTASIAVEPFLYAALLLEMAAMLVVPLLVPLYQRPGRGVTRFIIYQ
                     TLAMPFILFSGWMLAGVEASPGDLGTIVQSGVMVTMGFAFLFAVFPLYNWIPQLIEET
                     NPYAVGFLLWALPTFTTIFALGFLDRYTWLRNTPQISNAILFSGVIMTATGGIFGALQ
                     RHLGRIFGYAAIAETGLLLIALGLRSSEVVNIAFLILIPRGLELSVWALALSIIKRKA
                     YPLKFSDVQGLARKYPVATSALIIAHLSVAGFPLLAGFPSRLALWQELSLQSLSASLW
                     VFLGLLGLLVASVRTLATLVMASDDAPWELNETWVQTFMLGIGVLGIFLLGLFPQILQ
                     PFLSSLPALFEHLIQ"
     gene            2948..>3427
                     /locus_tag="PKI33_06295"
     CDS             2948..>3427
                     /locus_tag="PKI33_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36651.1"
                     /translation="MEFEQIVKRLEFLDKQQRETKETLTSLKESLASFETTVNAVSKQ
                     IKTLSKQVTDITPAAKRVDQFETMVGKQRNELVKMIEDVDKAHTRSERDTTKQFQSDI
                     NDLSKTLTQLKVSTDLTEIKKQLKERGNEIQRALANIADFKTVVEDTKRSNEDVKLSI
                     "
BASE COUNT          748 a          832 c          820 g         1027 t
ORIGIN      
        1 ttcaacttta cgccgcgggt ttggaaccgc cttacgtttg atcggcgcag catccaccct
       61 tagcattctc gggcatattc aaattcttcc aaccaacctg accccgatat tgatgatcct
      121 tacttcggtg gctgccattt atggtggttg gacttggctg cgcgcgcccg atgaattaaa
      181 cggtcgtcca tattggatga tcggcatggc ttcgcttgcg attctctcag cgttaagcgg
      241 gaatgccaca ggtgcaattg catggggtag tgcgcttgta ctcgtaggcg gtgcactctt
      301 tcttttttcc attcaagata tacgtctaag tcgtgcctta ctcattggtg gatgggcgct
      361 ttcctctctg cctctctcgc tcacagccag cacatgggcg agcagtttta acatctcgac
      421 tattttttca ttagcggcac aggtattact taccgctggg tatattcggc atgccttgcg
      481 caggaatggc accgagactt tggacgatca acccggttgg gcaaaggtcg tttacccagc
      541 tggcattctc ctgcttcttg tgactcaaat tctgcttgga tttatcggtt gggatggtgc
      601 tttcaaattc ggaaatttga ttttctcctt gctcgcctcc atccttgctt tgggcttggt
      661 cggtggaagc agacggtttc gcatcttcaa cccggtccgt gcacattggg tcactaccgc
      721 tggctcaggg gtcaatggac tttatcaatg gctctggtcg ctctatcgtg ggcttgccca
      781 agtagcacaa gccattacca atgccctcga aggggaaggc ggcatcatgt ggacgttgct
      841 cttcttaatt ctatttatct caatcatcgt acagggcatt caataaatgg tctctgatat
      901 tgtttcttgg atatcggtcg cattgaccct tgtcacagcc acaatgatcc ttgtgaatcg
      961 tgattggcgc atcagcttgg gtgcgcttgc tcttcaatat cttgccgcat tctggttagt
     1021 gactcgtcac ttgcctttcg cgatggggtc ggtcaaattg attaccggct ggatggtggt
     1081 agctattctc ggcatgacgc gtctcagctt gaccaatctt gatcaaaatg aacaagatgc
     1141 tttttatccg cgcggtgcag cgtttcgcat cacgttgatg ggaattgtca cgctcgtagc
     1201 attaggatcc acaccacgca ttgaagaagc cattccgggg cttggcatgc ctgtcatcgc
     1261 aggcggatta ctactgatcg gtgcaggggt ggcgcacctt ggagtcacta acgatctatt
     1321 acgtgtggcg ctcggtctac tgactgtgct cactgggttt gagattattt atgcagccgt
     1381 ggaaagtgcc attcttgtta cgggtctttt ggcagttgtg aatttagggt tgggcgtgat
     1441 gggctcctat ttattgatgg caggttctat tccacttatt tcccccgaag aagaggaaga
     1501 actgctatga gtgcccccat tctatggatt gccgttcctt ttgtgctcgg tgtattaatc
     1561 ctattcgtat taagcgaacg ctatgctgca tatgtcggtg ggacgatatc aactattctg
     1621 gcagcaattg cctttttcat tccaattgat accgcccttc tcttgggaaa tgtctccctt
     1681 aagatttctc cggctattga gttttttggt cgcagttttg agctctccac agcagatgga
     1741 cggttattaa cgctgatcta tggcttggct gctctctggt ttttcggcac cgaagcttct
     1801 ggtacggcac atcgttttgt ctctttggga ttaatgatca ctgcattgct aactgcttct
     1861 attgcagtag agcctttctt gtatgccgca ctcttgttgg agatggcagc catgttagta
     1921 gtaccactgc tcgttccgct gtaccagcga ccgggtcgtg gtgtgacccg cttcattatt
     1981 taccagacgc ttgccatgcc gttcatcctc ttttctggct ggatgcttgc aggcgttgaa
     2041 gcgagtccag gagacttggg gacgattgtt caatctggtg tgatggtgac gatgggcttc
     2101 gcctttctct ttgccgtctt tccactctac aattggattc cacaactgat agaagaaacc
     2161 aatccgtatg cagttggttt tctactttgg gcgttgccta catttacaac catctttgct
     2221 ttaggctttt tagaccgcta cacgtggctt cgcaatacac cacaaatttc aaacgctatt
     2281 cttttctcag gtgtcatcat gaccgctact ggcggcatct tcggcgcgtt gcaacgtcat
     2341 cttgggcgca tctttgggta tgccgctatt gcagagacag ggctattatt gatcgcgctc
     2401 ggcttacgct cttctgaggt tgtcaatata gccttcctca tcctcatccc tcgcggactt
     2461 gagttaagcg tttgggcatt ggcactatcc atcatcaaac gaaaagccta tccgcttaag
     2521 ttcagtgacg ttcaaggatt agcgcgaaag tatccagtag caacgagcgc attgataatt
     2581 gcacatcttt ctgtggcggg gtttccgctt ttggcaggct ttccctcgcg gttggcgtta
     2641 tggcaagagc tatcgcttca atcgctaagt gcttcattat gggtctttct tggcttatta
     2701 ggattgttgg ttgcctcggt ccgcacattg gcaacactcg ttatggcaag cgacgatgcg
     2761 ccgtgggaac taaatgaaac ttgggttcag acctttatgc taggtatcgg tgttcttggt
     2821 attttcctgc tgggactctt tccccagatc ctgcaacctt tcctttctag tttgcctgcc
     2881 ttattcgaac acctcattca ataatcccgt tttgcaaaag ggtataattt aacgaaacgg
     2941 agtcctcatg gaatttgaac agatcgtaaa aagacttgaa tttttagata aacaacaacg
     3001 cgagactaag gaaaccctta cctcgttaaa agaaagcctt gcctcctttg aaactacagt
     3061 aaatgccgtt tcaaagcaga ttaaaaccct gagcaagcag gtcacagaca ttacccctgc
     3121 cgccaagcgt gtagatcaat ttgagaccat ggttggtaaa cagcgtaatg agctggtcaa
     3181 aatgatcgaa gatgtggaca aagcccacac tcgttcagag cgcgatacca caaaacagtt
     3241 tcaatctgac ataaacgatc tcagcaaaac attgactcag ctaaaggtca gcaccgatct
     3301 cacagaaatc aagaaacagc tcaaagagcg cggtaatgaa attcaacgcg ccttggccaa
     3361 tattgcagac ttcaaaacag ttgtagaaga tacgaagcgc tcgaacgaag atgtgaagtt
     3421 atccatc
//
LOCUS       DAOREF010000245         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17616_length_3419_cov_4.345125, whole genome shotgun sequence.
ACCESSION   DAOREF010000245 DAOREF010000000
VERSION     DAOREF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17616_length_3419_cov_4.345125"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PKI33_06300"
     CDS             <1..744
                     /locus_tag="PKI33_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNM36652.1"
                     /translation="MQNRERQRITRDMQTRAEEIALNDDPNAHLLFAAHEEFNSGVVG
                     LAASRLTEKYYRPAIVASKGEEETRGSCRSIPEFHITDALDQCADLLVRHGGHAAAAG
                     FTVRNENIEALVSKLKAIAEEKLAGSDLKPTVTADAEVDLSEMRPEVYEKGLKYLEPT
                     GYGNREASFVARNVKVKSSRIVGSDGKHLKMSLEDSKGIAHDAIGFRLGDWHKSMPPR
                     VDVLFTYEPNEYNGRVTYQLNLKDLKASR"
     gene            complement(768..1595)
                     /locus_tag="PKI33_06305"
     CDS             complement(768..1595)
                     /locus_tag="PKI33_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM36653.1"
                     /translation="MDIKGKVVIITGASSGIGAAAALEFGREGAKVVLAARRTDKLQE
                     LVNEIRAMQNGAEAFVLQTDISKLDDIQRLVAQTVERFGRVDVLVNNAGFGRLKWLET
                     LDPLEDIQAQFDVNVLGVIQTTRQVLPIMIKQRSGSIINICSIAGLVGTPTYTIYSAT
                     KHAVHGFSEALRREVKPWGIDVSLIYPGGVRGTEFPDHAGIQRKTNASTPKFMVLTAE
                     QVGKAVVNLVHRPRRMWVLPWMWRFTIFLNRFLPGLVDRTTIKNFTIPERADELNKK"
     gene            complement(1612..2613)
                     /locus_tag="PKI33_06310"
     CDS             complement(1612..2613)
                     /locus_tag="PKI33_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36654.1"
                     /translation="MKTKRPAWWILFLIVFVFACNLPSASTPTPDLFATLSAVTPAPA
                     DSTSQPNPSTSSTAEPTGKIVFVCQIFKTQASNQICIINADGSGFRRLTTDNTRQHYY
                     PSLSPDGKSVVYAAFREPNIYEIYEMDIATGNVAQLTDRLGNLNGPEISPDGSMIAFK
                     LSTAAANQIWLMNRDGSDPHPIPNAFGWDPTWSPDGTSILFASDMQGVVQLYVIKLDG
                     SELQKISDLPAIRGRSDWSPNGQFIVTYSGQPWNRDVYIMNADGSDARMLTPTGGNSQ
                     GPSISPDGQWVVFTSYYDHYGEDHGCEIYIMPVNDTQQVVRRLTDNDYCDYQPRWGP"
     gene            complement(2646..3096)
                     /gene="rnpB"
                     /locus_tag="PKI33_06315"
     ncRNA           complement(2646..3096)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI33_06315"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          866 a          855 c          849 g          849 t
ORIGIN      
        1 atgcaaaacc gtgaacgtca acgcattacg cgcgatatgc aaacccgtgc cgaagagatc
       61 gcattgaatg atgacccaaa tgcacatcta ctctttgccg ctcatgaaga gttcaactca
      121 ggggttgtag gtctcgctgc ctcacgcctc accgaaaaat attaccgtcc cgctattgtc
      181 gcttcgaaag gcgaagaaga aacgcgtggc tcttgccgtt ccatcccaga atttcacatc
      241 accgatgcgc tcgatcaatg cgcagacttg ctcgtgcggc atggtggtca tgccgcggcg
      301 gcagggttta cagttagaaa tgaaaatatc gaagcacttg tctcaaagct aaaagctatc
      361 gccgaagaaa agctcgctgg aagtgacctc aagcccaccg ttacggcaga tgcggaagtg
      421 gacttatccg aaatgcgtcc agaagtgtac gagaaaggct tgaagtatct cgaaccaacc
      481 gggtatggca atcgtgaagc gtcgtttgtg gcacgcaatg tcaaagtcaa aagttcgcgc
      541 attgtgggtt ctgatggcaa gcatttgaag atgtcattgg aagattccaa aggcattgct
      601 cacgatgcga tcggctttcg ccttggcgat tggcacaagt ccatgccgcc acgggtggat
      661 gtgcttttca catatgagcc aaatgaatac aacgggcgcg tcacttatca attgaatttg
      721 aaagacttga aagcttcaag ataaaaaata gacagccttt cggctgtcta ttttttattc
      781 agttcatcgg cacgctctgg aatagtgaag tttttgatcg tagtacggtc cactagccct
      841 ggcaaaaaac gattgagaaa aatagtaaag cgccacatcc aaggcaatac ccacatacgg
      901 cgcggacgat gcacaagatt caccacagct ttgcctactt gttccgctgt taataccatg
      961 aactttggcg tggaagcgtt ggtcttgcgc tgaataccgg catggtcggg gaattctgtt
     1021 ccacgcacgc cgccgggata gatgagagag acgtcaattc cccaaggttt gacttcgcgc
     1081 cgcaaagctt ctgaaaagcc atgcacggca tgtttggtag cagagtaaat tgtataggtc
     1141 ggcgtgccca ccaaaccagc aatggagcag atgttgataa tactcccact gcgctgtttg
     1201 atcatgatcg gcaaaacttg tcgtgtggtt tgaataacgc ccaaaacatt cacgtcaaat
     1261 tgtgcttgaa tatcttcaag tgggtcgagc gtttcgagcc atttcaaacg tccaaagcct
     1321 gcattattca ccaacacatc cacacgacca aatctctcca cggtttgagc aaccaaacgt
     1381 tgaatgtcgt ccagttttga gatatcggtt tgaagtacaa aggcttctgc gccattctgc
     1441 attgcacgaa tctcattcac caattcttgt aacttgtcag ttcgccgtgc agctaacaca
     1501 accttcgccc cttcacgccc aaattcaagc gcagccgctg ccccaatccc cgacgaagcg
     1561 ccggtgatga ttactacttt tcctttgatg tccatgttgt ccttgttgtt cttaaggtcc
     1621 ccagcggggt tggtaatcac aataatcgtt gtctgttagt cggcgtacaa cttgctgtgt
     1681 gtcattcaca ggcatgatgt aaatctcaca tccatggtcc tcgccataat ggtcataata
     1741 agaggtaaac accacccact gcccgtctgg tgaaatggat ggaccttgtg agttaccgcc
     1801 cgttggtgtg agcatgcggg catccgaacc atctgcattc atgatgtaaa catcgcgatt
     1861 ccaaggctga cccgaatatg tgacgatgaa ctgtccattc ggtgaccaat cgcttcttcc
     1921 ccgtatcgcg ggcaggtcgc tgatcttttg cagttcgctt ccatcgagct taatcacata
     1981 aagctgaacg acaccttgca tatcagaggc aaacaaaatg gaggttccat ctggagacca
     2041 agttgggtcc cagccaaaag cattgggaat gggatgcggg tcgctcccat cacggttcat
     2101 caaccaaatc tgattggctg ccgcggtgga gagtttgaac gcgatcattg aaccatcagg
     2161 cgaaatttcc ggtccgttga gattgccgag acggtcggtt agttgagcaa cgttgccagt
     2221 ggcaatatcc atttcgtaga tttcgtaaat attcggttca cgaaatgctg cataaacaac
     2281 acttttgcca tctggcgata gcgatgggta ataatgttga cgagtgttat cggtggtcaa
     2341 acggcgaaaa ccagagccat cagcattgat gatgcaaatc tgattactgg cttgtgtttt
     2401 gaatatctga caaacgaaaa cgatcttgcc agttggctcc gctgtggatg aggttgatgg
     2461 gttaggctga gaggtagagt ctgctggggc gggggtaact gctgatagag tggcaaacag
     2521 gtctggggtt ggggtagaag cggagggtaa attgcaagca aagacgaaaa cgattaagaa
     2581 tagtatccac catgcagggc gtttggtctt catccgattt aaagaattca tccaataaaa
     2641 aaatcgcttc aggctggtct ataagccgca ttctgtccaa tggcttgcgc catttgggca
     2701 atcatctctc tgggcggtgc gtcaccgcgc cgctcggtgc agcctacccg ggactgaccc
     2761 aacccacagg agggaagggt gcgtatggag gcgagcagcc tcccatcgtc cacagacgaa
     2821 atcgtccctg cttggccttg ctcccggcgg gggttacctg gccacgcaca ttactgcgcg
     2881 cgccggtggt ctcttacacc accttttcac catcactgcc aaaggcagct gtttgtttct
     2941 gtggcccttt tccggtaggt tcatgccttt cggcaatctt tccgtcaacg aatgacggac
     3001 ttccccaccc cgggtgctat ccgacgccgt gctctaaagg agtgcggact ttcctcgatc
     3061 ccgtcaacgc agaaccgcga ttgcccaacc aacctgaagc gtttgcatga tactcgtgga
     3121 gagagggaga gtcaaccgcg cagagccgat tttgctcttt tctttataca attttgcaaa
     3181 agtcatgcag ttttcattgc caaaggtgga catttctgga aaagacatta aaattgaaag
     3241 gtcatataat ttttgtctcc tagaggaatc cctttgagca acatacctgg aaaattcgtc
     3301 attggtctca ctggtaacat cgccaccggc aagagtgtgg tgcgccgcat gctggaacac
     3361 ttaggcgcat acaccatcga tgccgattct ttgacgcacc gttcgtacgc caaaggcgc
//
LOCUS       DAOREF010000246         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17632_length_3418_cov_4.467142, whole genome shotgun sequence.
ACCESSION   DAOREF010000246 DAOREF010000000
VERSION     DAOREF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17632_length_3418_cov_4.467142"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1370
                     /locus_tag="PKI33_06320"
     CDS             135..1370
                     /locus_tag="PKI33_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNM36655.1"
                     /translation="MTLFLQLAFLLSVILLSAKMAGYLSIRFGQPSVLGELLVGILLG
                     PSIINILNLPFIEHELTETIVKLGELGVLLLMFLAGLELHLGEMRQNVRVAALAGLMG
                     VVAPVLLGWGTGLLFGFNQAASIFLGLTLGATSVSISAQTLIELKVLRSRVGLSLLGA
                     AVFDDILIILLLSVFLALQGDAGSTANVLLIIVRMVLFLVASALFGIWALPWIVRRVA
                     HLPISQGLLTISLVIMLAYGIASEAFGGMAAITGAFLAGLMLARTPEKERIEAGVHAL
                     AYGLFVPVFFVNIGLSINLRDFHADAFLMTLAIIFVAIAGKWIGSGWGAKLGGLSSHE
                     ATQLGAGMISRGEVGLIVASVGMRQGLVVDAEFSAIVVMIVVTTLITPPILRGLFARE
                     TPKHKPASQIEKEKPSEAS"
     gene            1374..1733
                     /locus_tag="PKI33_06325"
     CDS             1374..1733
                     /locus_tag="PKI33_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36656.1"
                     /translation="MYLVTLVLHDPDLLEELLDAWNKAGVEGATVLFSSGMGRFRQKQ
                     GMRDDLPLIPSLDDFYEAPQTLNRTVFTVVHDDAMVDKLLEVTQTLVGDLSKPDTGIM
                     VVTPVVRAYGLDPIDKK"
     gene            1935..2270
                     /locus_tag="PKI33_06330"
     CDS             1935..2270
                     /locus_tag="PKI33_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNM36657.1"
                     /translation="MEHPTFLLLMAVVQEQDLNAATTALQKIGASSTYLSSAGGFLGR
                     RNATMLVGLPADKLQDSLKVLHEACRQRIEYMTLPLEGSPMPMPAPVPVTVGGATVFA
                     LPVERFEQI"
     gene            2292..2552
                     /locus_tag="PKI33_06335"
     CDS             2292..2552
                     /locus_tag="PKI33_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNM36658.1"
                     /translation="MKMIIAIVHDNDADILTQAFTAASFRVTRVASTGGFLRSGVVTM
                     LLGVDDDQVDAVIETVRKALPSKGDKKRATVFVVPVSHFEQV"
     gene            2633..>3418
                     /locus_tag="PKI33_06340"
     CDS             2633..>3418
                     /locus_tag="PKI33_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36659.1"
                     /translation="MKNKKIFAFLALLLLTLACANPLTTPAEQPANVETIVAATISAL
                     TFTPAPSTPVVGDSASSLLPHSIYYLAPDSAQITQVFRLEKDGKTITQLTFEPSKVED
                     FDVSLADGRIVFLTNHQLYLANADGSNRLLIFDAGPQDEVNMFLTRIISPVFSLSGQT
                     IAFGHQGLNFYSLTSGQTNRVLEDKVNDPGQGFIIPEELYWPEMYTTNENKLILTLGY
                     YEGASTGFYDISSGTLTRLDNEQRAIICCGDYSLSADSSSLYAG"
BASE COUNT          835 a          888 c          811 g          884 t
ORIGIN      
        1 gaatatttaa ataggaagag ataaacgatt caaagcaaga gacagtcatc aagcacataa
       61 ctgtctcttt ttgtttaaag tggaattaat cttaacgaaa aaagttcatg gtatacaaaa
      121 tctcaaggtg aaccatgact ctatttcttc aacttgcctt tctactttca gtgatcctgc
      181 tctctgccaa gatggcagga tatctcagca ttcgcttcgg tcaaccttcg gtgttgggcg
      241 aattgctggt gggaattctg ttgggtccat cgatcatcaa catcctcaac ctgccgttca
      301 tcgaacacga actcaccgag accatcgtca aacttggcga actcggcgtg ctgttattga
      361 tgttcctcgc cgggttggaa ttgcatctcg gtgagatgcg ccaaaacgtg cgtgtggcag
      421 cattggcagg gttaatgggc gtggtcgcac ccgtcctttt gggatggggc accggcttgc
      481 tctttggatt caatcaagcc gcctcgatct ttcttgggct cacgctgggt gccaccagtg
      541 tcagtatctc cgctcagacc ttgatcgaac taaaagtgct acgcagccgg gtggggttga
      601 gtctgctcgg agcagccgtg ttcgacgaca tcctcatcat tctgttgctc tcggtcttcc
      661 tcgctttgca aggagacgca ggcagcaccg ccaatgtttt actaatcatc gtacgcatgg
      721 tgttgtttct tgtggcatct gctttgtttg gcatttgggc attgccatgg atcgtacgcc
      781 gcgtagcgca tttgcccatc agtcagggac ttttgacgat atccctcgtc atcatgttgg
      841 catatggaat cgcatccgaa gcctttggcg gcatggctgc tatcacgggc gctttcctcg
      901 ccgggctaat gttggctcgc accccagaaa aagaacgtat tgaagccgga gtccatgcct
      961 tggcatatgg gttatttgtg ccagtattct tcgtcaacat cggcttatcc attaacctgc
     1021 gagacttcca cgccgatgca tttcttatga ctctggcgat cattttcgtt gccattgcag
     1081 gcaaatggat cggctcgggc tggggtgcca aactaggcgg actcagctca cacgaagcga
     1141 ctcaactcgg cgcaggcatg atctcacgcg gcgaagttgg gctgatcgtt gccagtgtgg
     1201 gcatgcgcca gggtttggta gtggatgctg aattttcggc aattgtggtt atgatagtag
     1261 taaccacttt aatcacaccg cccattctac gcgggctgtt cgctcgggaa accccgaaac
     1321 ataaacccgc ttcacaaatt gaaaaagaga aaccatcgga ggcatcctaa tttatgtatc
     1381 tcgttacgct cgtattgcac gaccctgacc tattggaaga acttctggat gcgtggaaca
     1441 aagccggtgt ggaaggtgcg accgtcctgt tcagctcggg catgggcagg tttcgccaga
     1501 agcaaggcat gcgtgatgat cttccgctca tcccttcgtt ggatgatttc tacgaagcac
     1561 cgcaaaccct caaccgcaca gtctttaccg ttgtccatga tgatgccatg gtcgacaaac
     1621 tcctcgaagt cactcaaacg ctggtcggtg acttgagcaa acccgatacc ggcatcatgg
     1681 tcgtcacgcc ggttgtccgt gcctatgggc ttgaccctat agataaaaag tgatcgttca
     1741 taaaaagcag ccacagaatg caaactctgt ggctgctttt tcatattgca taggttctag
     1801 gttgaagcat cccccgagtg atgataatat caaagcagag ataaaaccgt cgaaacagaa
     1861 tcaagcccgg gcgccgaaag acgcgcaagg tctaaaatcc aattcaatca aatcttgatt
     1921 gaaggagaag agtcatggaa cacccaacct ttttattatt gatggcagtg gtgcaagaac
     1981 aagacctcaa tgccgccacc acagccctgc aaaagatcgg cgcatcatcc acttatcttt
     2041 cgagtgcagg cggtttctta ggcaggcgca atgccaccat gttggtaggc ttacccgccg
     2101 ataaactgca agactcattg aaggtcttgc acgaagcctg tcgtcagcgc atcgagtaca
     2161 tgacgctccc cctcgaaggt tcgcccatgc ctatgcctgc accggtgccc gttacagtgg
     2221 gtggcgcaac cgtctttgca ttgcctgtgg aacgcttcga acaaatttag aatcaaagag
     2281 gagaacctcc catgaaaatg atcatcgcca tcgtacacga taatgacgcc gatatcctaa
     2341 ctcaagcgtt tactgctgcc agcttccgtg tgactcgtgt ggcatctacg ggcggctttt
     2401 tgcgcagcgg ggttgtcacc atgctcctcg gcgttgatga cgaccaagtg gacgctgtga
     2461 ttgaaactgt ccgcaaggcg ttgccatcca aaggggataa aaaacgcgcc actgtttttg
     2521 ttgtgcctgt cagtcatttt gaacaagtgt aaagcgtgta taaacaaaga cgttgtgtca
     2581 gcaggaacat tccagccggt acaacgtctt tgttttgttt aacgaggtga ttatgaaaaa
     2641 caaaaagatc tttgcgttcc ttgctctttt attacttacc cttgcatgcg ccaatccatt
     2701 gacaacacca gccgaacaac cagccaacgt ggaaacgatc gttgccgcaa ccatttctgc
     2761 attgacgttc actccggctc cttccacgcc cgtagttggt gattccgcat ccagccttct
     2821 gccacactcc atttattatc ttgctcctga ctctgcccag atcacacaag tctttcgttt
     2881 ggaaaaagat ggcaagacca ttactcaact caccttcgaa ccctccaaag tagaagactt
     2941 cgatgtttct ctcgcagatg gcagaatcgt tttcctcacc aatcatcagc tgtaccttgc
     3001 aaatgcagat ggcagtaacc gcctcctgat cttcgatgca ggtccgcaag atgaagtcaa
     3061 tatgtttcta acgcgcatta tcagcccggt cttttctctc agtggacaaa ccattgcctt
     3121 cggtcaccaa ggcttgaatt tttactccct cactagcgga caaaccaacc gcgtgctgga
     3181 agataaagtc aacgaccctg ggcagggctt catcattcca gaagagttgt actggcccga
     3241 aatgtataca accaatgaaa acaaattgat cctcacattg ggatactacg aaggcgcctc
     3301 tactggattt tatgacatca gcagcggcac attgactcga cttgataatg aacaacgagc
     3361 gatcatctgt tgcggagatt actcactgag cgctgattct tccagtttat atgcaggc
//
LOCUS       DAOREF010000247         3417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17646_length_3417_cov_3.229923, whole genome shotgun sequence.
ACCESSION   DAOREF010000247 DAOREF010000000
VERSION     DAOREF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17646_length_3417_cov_3.229923"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PKI33_06345"
     CDS             <1..1575
                     /locus_tag="PKI33_06345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM36660.1"
                     /translation="QREFQNTVYEYQPAVTTMEIISLAMFLYFPEYRGLGQGYLDYEK
                     GELDPFMYKHGYDPLVLLAYSRTIQIFVLLILWLILYCLLLRFFTKWVAVFAVLLIAF
                     DPYYLGLTRIMTHEAMVALFILVSLVSLAVYFFRDQNFIFLLISGVAAGFAQLTKSSA
                     IAMLGAIGVLLLFQVFQGQKGNWQKNVLHAFQTFIKWLLILALTYVVFWPGMWVAPGK
                     MLYQVYGNAFSYAFQGARLTVTEDLTPSEFSLNTAPDNTWDVASVLLYRTTPIIWVGG
                     ILGLIFLFYSEHGKILNYRVLAAFLLTNTIAFVLLIGLAQGRNSPHYILSSYVSLDVL
                     AAMGWVALFQRFTFKTTVLSINSMQAIVMAILVVFQVFSSTLKFPYYFTYRNPILYAA
                     GWYQEYPQKPYGEALEMAADYLSKQPDASDSTAFVYYSRGCFSYFYPGKSISFRPYYI
                     DGDHSEDLMNNLNSSDYLVVYYANQVQFEKYHPFLNILAKSEPYHVIWMDGYEYVRIY
                     KVDTLSSEILEALAGL"
     gene            1572..3281
                     /locus_tag="PKI33_06350"
     CDS             1572..3281
                     /locus_tag="PKI33_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNM36661.1"
                     /translation="MKLNKNIWFYILFLVFALPSWLIQNDRVINVDEPRWIVRGANFY
                     YAVTHGEFEKTIFEYHPGVTNMWIIGTAMHIYFPEYRGMGQGYFDPLKTKFEEFIREN
                     DKDVLEIVRISRWLHTGVLAAFFLIALFLLSKLVDIKVAFLSVLLAAVSPFYLGHSRL
                     MNLEGMLAIFVLVSLLGLYIYTNKEKNLFFLILSGVCFGLAQLTKSSSIVLLGTVGIV
                     FLISIFKKNEELFYSKLLVGIRTFLIWFVSAVITYFVLWPGMWVAPVKMVSEVYGNAF
                     SYAFQGARLDVKEELAPESFNVETDNNGALNFAEKWMSSTTIVSWLGLLLVVVFLMQK
                     DSLAFSIKDAIGYLFLLAISFMVLFGLAQGRDSQHYILSSYVAFDVIAGIGWGSALLW
                     AGSRWKGLASTTASVVVLVVITLAQIGFGLPYAPYYFTYKNPFAKEAATYGYGEGFSE
                     AADYLAQKPNAKELTAYVYNGMGTFSYFFPGKTVVLKRANVLYGDFVTITDELRSSDY
                     LVLYPIVREKQPETEKIFSELEGVVKPEKVIYINGLEYIQIYKVTDIPETVYEKLLAK
                     NEP"
BASE COUNT          850 a          579 c          799 g         1189 t
ORIGIN      
        1 cagagggaat ttcaaaatac ggtgtatgaa tatcagcctg ctgtaacaac gatggaaatc
       61 atttctctgg cgatgttttt atactttcca gagtaccgcg ggttgggtca aggctacctc
      121 gattacgaaa aaggtgaatt agacccattt atgtataagc atgggtatga tccgctcgtt
      181 ttattggcat attcacgaac cattcaaatt tttgtgctgt tgatattatg gctgattctt
      241 tattgtctgt tgttaagatt ctttaccaag tgggtagctg tttttgctgt tcttctcatc
      301 gcatttgacc cgtattatct tgggcttact cgcatcatga cgcatgaagc aatggttgct
      361 ttgtttatat tggtgtcttt ggtgtctttg gctgtatatt ttttcagaga ccagaatttt
      421 atttttttgt tgatttctgg tgtggcagcg gggtttgctc agttgacaaa atcatctgcc
      481 attgcaatgc taggcgccat tggggtattg cttttgtttc aagtatttca ggggcaaaaa
      541 ggtaattggc aaaaaaatgt gttgcacgct tttcagacat ttatcaagtg gctattgatt
      601 ttggcgctga cttatgtagt gttttggccc gggatgtggg ttgcacctgg caaaatgctc
      661 taccaagttt atgggaatgc ctttagttat gcttttcagg gagctcggtt aacagttact
      721 gaagatttaa ccccttcaga atttagccta aatacagcac cagataacac ttgggatgtt
      781 gctagcgttc ttttgtatcg aacaacaccc atcatatggg taggtggaat attaggattg
      841 atttttctgt tttattctga gcatgggaag atacttaatt atcgtgtatt ggcagctttt
      901 ctgttaacga atacgattgc ttttgttctg ttgatcggct tggcgcaagg gcgtaattca
      961 ccacattata ttttgtccag ctatgtttct cttgatgtat tggcagctat ggggtgggtc
     1021 gccttgtttc aacgatttac tttcaaaacg actgtcttgt ccatcaactc tatgcaggcg
     1081 atagtgatgg caattcttgt cgtttttcaa gtgtttagtt ctactttgaa gtttccttat
     1141 tactttacat atcgaaaccc aattctgtac gctgcgggct ggtatcaaga atatcctcag
     1201 aaaccctatg gtgaggcttt ggagatggca gcagactatt tgtcgaaaca gccggatgca
     1261 tctgattcaa cagcctttgt ctactatagt agaggatgtt tttcttattt ttaccctggt
     1321 aagtccatta gctttcgccc ttattacatt gatggtgatc attctgaaga tttgatgaac
     1381 aacttgaata gctcagacta tttggttgtt tattatgcaa atcaagttca atttgaaaaa
     1441 taccatcctt tcttgaacat tcttgccaag tctgaacctt atcatgtgat ttggatggat
     1501 ggctatgaat acgtccgcat ttataaggtg gataccttat cttctgaaat acttgaggct
     1561 ttggctggtt tatgaaatta aataagaaca tatggttcta tattcttttt ctggtgtttg
     1621 cccttccgtc ttggttaata caaaacgacc gggttattaa tgttgatgaa cctcgttgga
     1681 tcgtccgtgg agctaatttt tactatgctg ttacgcatgg ggagtttgaa aaaactattt
     1741 ttgaatatca tcctggtgtt accaatatgt ggatcattgg taccgccatg cacatatatt
     1801 tccctgaata tcgtggtatg ggacaaggat attttgaccc cttaaaaaca aagtttgaag
     1861 agtttatacg cgagaatgat aaagatgtcc tggaaattgt tcgaattagc cgttggcttc
     1921 atacaggagt attggctgct tttttcttga ttgctttatt tcttttatca aaacttgttg
     1981 acataaaagt agcgtttcta tctgttttat tagctgctgt ttcaccgttt tatctgggtc
     2041 actcccgatt gatgaatctg gaagggatgt tggctatttt tgtgctggta tcccttttag
     2101 gattgtatat ttatacaaac aaagaaaaga atcttttctt ccttatactt tctggggttt
     2161 gttttgggct tgcgcaacta acaaaatcgt cttctatagt gcttcttgga acagtgggta
     2221 ttgtgttctt aatatccatt ttcaagaaaa atgaagaact gttttactct aaattattgg
     2281 tcggaattcg aacttttttg atttggtttg tttctgctgt aataacctat tttgttttat
     2341 ggccaggcat gtgggtggca ccggtcaaga tggtttctga ggtgtatggc aatgcgttta
     2401 gctatgcttt tcagggcgca aggcttgatg tgaaagagga actggctcca gaaagtttta
     2461 acgtagagac agacaacaat ggcgcgttga attttgctga gaaatggatg tcttctacaa
     2521 caatagttag ttggctgggt ctactattag tcgtagtttt cttgatgcaa aaagatagtt
     2581 tggctttcag tatcaaagat gcgattggat atttgttttt attagcaata tcttttatgg
     2641 tgttgtttgg gctggcacag ggacgtgact ctcaacatta cattcttagt agttatgtcg
     2701 catttgatgt cattgcggga atcgggtggg ggagcgcatt attgtgggcg gggtcgcgct
     2761 ggaaaggact ggcgagcaca actgcgtctg ttgtggtttt ggttgtgatt acgcttgctc
     2821 agattgggtt tgggttgccg tatgcgcctt attactttac gtacaagaat ccgtttgcta
     2881 aagaggcggc gacatatggg tatggcgagg gtttttctga ggcagcagat tatttggcac
     2941 aaaagccaaa tgcaaaagag ttgacggcat atgtctataa cggtatgggc accttctcgt
     3001 atttcttccc tgggaagacc gtcgttttga aacgcgccaa tgttttgtat ggtgactttg
     3061 tgaccattac tgacgaacta cgaagctctg attatttggt tttgtatccg attgttcgag
     3121 aaaaacagcc ggagacagaa aaaatcttca gcgagttgga aggggttgtg aagcctgaaa
     3181 aggtcatcta catcaacgga ttggaatata tccaaatcta taaagtgacc gatattcccg
     3241 aaacagtgta tgagaagtta ttggcgaaaa atgaaccgta agatattaaa cggcgcgatg
     3301 agcgccgttt tgcttgggct acagagtagg ggattttagg gtgggagaaa caggcaaaag
     3361 tctagaaaga ggcgggtttt cctatcccac cttacgcctt tgtcatgaaa gtaccat
//
LOCUS       DAOREF010000248         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17718_length_3408_cov_3.487623, whole genome shotgun sequence.
ACCESSION   DAOREF010000248 DAOREF010000000
VERSION     DAOREF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17718_length_3408_cov_3.487623"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PKI33_06355"
     CDS             <1..1172
                     /locus_tag="PKI33_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36662.1"
                     /translation="LGQDIQDSVGSWKWFENPESFALVGSTINPRAAGFVAGVEYQSY
                     FRPNFSSYGPQAFANTKAIGANFAVLSPSWTISNTSPLRFSAQPGNDPFWIDTAIMVS
                     QARALGLNIALFPTPHFPPSTDSNISASDQFWKNAPKDAAWWQTFFTRYRAFLVNYAD
                     LTAQSGAQVIILGGDWITPALPGGLLPDGTSSNVPADAEAQWRSILQDVRSHFKGQIL
                     WALPYEVSSFTTPVSFLRDVDGIYLLWSTPIATSSTATKTDYANETGRLLDNEVAPLA
                     SLLVKPVILAVSYPSAAGAPTGCISNGSGACLDFDALSRPNVDLSSVSLNLQTQADIY
                     EAMLNAVNARPWISGFVSRGYYLPVVLQDKSTSIHGKPAADVLWYWYPRLLGTVQ"
     gene            1285..>3408
                     /locus_tag="PKI33_06360"
     CDS             1285..>3408
                     /locus_tag="PKI33_06360"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNM36663.1"
                     /translation="MKKIGRFLGRMLIGILIIAVVVTSGGLFYFKSYLPKTVAPKSFP
                     QIDGEIKVDGLDGEVNIYRDAMGIPHIYATTAHDIFFAQGYVHAQDRFWQMDAWRHIG
                     SGQLSEMFGEGQVETDAFLRTLGWRKTAEAEWEQLDSDSRAILQSYTDGVNAYLKDHS
                     GTALSLEYAVLGLLSPDYVIEPWEPVNSLTWGKAMAWDLRGNMGEEIERAELLKTLTP
                     EQVALLYPEYPQDHPVIVNKIGEGGQSASTSQPSSFDYSALNLDSLSHNASLLDPVLG
                     PLGDGIGSNSWAVSGALTDTGKPYLANDPHLGIQMPSIWYQVGLHCVEKTDSCPFEVA
                     GFSFAGVPGIVIGHNDKLAWGFTNVGPDVMDLYIEKVNPENPNQYEVNGKWVDFETYE
                     ETINIVGGEPVTITVKKTRHGPVISDTYGPTKNVGDPEDTKFIPFKDRVGGVELPEQY
                     VIALAWTALTPSTPFEAIWGFNKAQNWEDFRKAASDFHVPAQNLLYADVEGNIGYQMP
                     GDIPIRKNGDGTVPVPGWTDDYEWEGFIPFDELPYALNPEEGYIVTANNQVNPWDYPY
                     LITKDWDYGYRAQRIVDMIKNAPGKIDKEYIQSMQGDATDLNAESIVPVWKEIDYKAA
                     TKNEAYALDQLQNWDYTCKADSKACAIFQWFWWNLDQNTINDDVPERNAVSGGSKDFE
                     TFRHLLAEPDNFFWDDKLTTDVTETR"
BASE COUNT          901 a          833 c          795 g          879 t
ORIGIN      
        1 tgcttggaca ggatattcag gatagtgttg gttcgtggaa atggtttgaa aacccagaaa
       61 gttttgcgct ggttggtagc acaatcaacc cgcgagcagc aggctttgta gctggcgtag
      121 aataccaatc ctatttccgc ccaaactttt cttcgtatgg tccccaagca tttgctaata
      181 caaaagccat cggtgccaac ttcgctgtac tctccccgag ttggacaatt tcgaatacat
      241 cccctttgcg cttttctgct cagcccggta atgacccgtt ctggatcgat acagccatca
      301 tggtgtcgca agcgcgcgct ttaggcttaa atattgcgct cttcccaacc cctcatttcc
      361 cgccatccac agacagcaat atctctgcca gcgaccaatt ctggaagaac gctcctaaag
      421 atgcagcttg gtggcaaact ttcttcacac ggtatcgtgc cttcctagtc aactatgcag
      481 atctcactgc ccaaagcggt gctcaggtta tcatccttgg tggagattgg atcacgcctg
      541 ccctccctgg tgggcttctg cccgatggca catcttcaaa tgtcccggca gatgctgagg
      601 cgcaatggcg ctccattcta caagacgtac gctcgcattt caaaggtcag atactatggg
      661 cattaccata tgaagtttcc agcttcacta cccctgtaag tttcttgcgt gatgtagatg
      721 gaatttactt gttgtggtct accccaatcg caacaagttc cacagcaaca aaaacagatt
      781 atgcgaatga aaccggtcgc ttgctcgata atgaggtcgc tccgctggca tctttattgg
      841 tcaagccggt catccttgcc gtctcgtatc catccgcagc aggcgcccct acagggtgta
      901 tatccaacgg gtctggagcc tgtttagact ttgatgccct ctcccgccct aatgtagatt
      961 tatcatctgt cagtcttaac ttacaaaccc aggcagatat ttacgaagcc atgctcaatg
     1021 cggtcaacgc ccgaccatgg atttctgggt ttgtcagccg cggatattat ctccctgttg
     1081 ttttgcaaga taaatccaca tccattcatg gcaagcctgc ggcagatgtg ttgtggtatt
     1141 ggtaccccag gttgttagga accgttcaat aatttatttt cttcatttat gaatcagacg
     1201 cagagtgagc tacgaaactc tgcgtctggt gtattaagta ctgccacaga gcccatgact
     1261 ctgtggaaaa ccattggagg aactatgaaa aaaattggca ggtttttagg gaggatgtta
     1321 atcggaattt tgatcatcgc tgtggtggtc acctcgggcg ggctttttta tttcaaaagc
     1381 tatttaccga aaacagtcgc gccaaaatct tttccacaaa ttgacggaga gataaaagtt
     1441 gacggcttgg acggagaagt gaatatttat cgcgatgcga tgggcattcc gcacatctat
     1501 gcgaccacag cacacgatat tttctttgct cagggctatg ttcatgctca agaccgtttc
     1561 tggcaaatgg acgcctggcg tcatattggt tcagggcaac tctctgaaat gttcggcgaa
     1621 ggtcaagtag aaacagatgc tttcttgcga acgttgggat ggcgcaaaac agcagaagct
     1681 gaatgggaac aacttgactc tgattcacgt gccatcttgc aatcatatac cgatggtgtg
     1741 aacgcttatc tcaaagatca tagcggaacc gccctcagct tggaatatgc cgtgttgggg
     1801 ttgctcagcc ccgattacgt cattgaaccg tgggaacctg tcaattcgtt aacatggggt
     1861 aaagcgatgg catgggatct ccgtggcaac atgggtgaag aaatcgaacg tgcagaattg
     1921 ttaaaaactc tcacgccaga acaggttgca ttgctctacc cagaatatcc tcaagatcat
     1981 cctgtcatcg tgaacaaaat tggtgaaggt ggacaatctg catcaactag tcaaccttca
     2041 agctttgact attctgccct caatcttgat tcactctcac acaacgcctc attactggac
     2101 ccggtacttg gtccgcttgg cgatggaatt gggtcaaact cttgggctgt ctcaggcgca
     2161 ttgaccgaca ccggtaagcc ttatctcgcc aatgatccgc atcttggcat tcaaatgccg
     2221 tctatttggt atcaggtcgg tttgcattgc gtagaaaaaa cagatagttg tccgttcgaa
     2281 gtagctggat tctcatttgc tggggttccc ggtatagtaa tcggtcataa tgacaaactc
     2341 gcatggggct ttacgaatgt aggaccagat gtgatggatc tgtacatcga aaaggtcaac
     2401 ccagaaaacc ccaatcaata tgaggtcaac ggcaaatggg ttgattttga aacatacgaa
     2461 gaaaccatca acattgtcgg tggtgaaccg gtaactatca cggtaaaaaa gacccgccac
     2521 ggaccggtca tctcagacac ttacggtcca actaaaaatg ttggcgaccc agaagataca
     2581 aagttcattc cattcaaaga ccgtgtaggc ggagtggaac tgcccgaaca atacgtgatt
     2641 gccctcgcat ggacggcttt aaccccatcc acaccatttg aagccatttg gggctttaac
     2701 aaggcacaaa actgggaaga cttccgcaaa gccgcgagcg acttccatgt gcctgctcag
     2761 aacttgctgt atgccgatgt agaaggaaac atcggttatc aaatgccggg cgatattcca
     2821 attcgaaaga atggtgacgg aactgtgccg gttcctggtt ggacggatga ttacgaatgg
     2881 gaaggtttta ttccgttcga tgaattgccc tacgcgctca accctgaaga agggtatatc
     2941 gtcaccgcaa acaatcaagt caacccatgg gattatccat acctcatcac caaagattgg
     3001 gactatggtt atcgtgctca acgcattgta gacatgatca agaatgcccc cggcaagatt
     3061 gataaagaat acattcaatc tatgcagggt gatgcaacag acctgaacgc ggaaagcatc
     3121 gttccggttt ggaaagagat cgattacaaa gccgccacca aaaacgaagc ttatgcgctc
     3181 gaccagttgc aaaattggga ttacacctgt aaagctgact ctaaagcctg cgcaattttc
     3241 caatggttct ggtggaatct tgaccagaac acgatcaacg acgatgttcc ggaacgtaat
     3301 gctgtcagcg gcggctcaaa agatttcgaa accttccgtc atcttttggc ggaacctgat
     3361 aacttcttct gggacgataa acttacaacc gatgtgactg aaactcgc
//
LOCUS       DAOREF010000249         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17766_length_3402_cov_2.570362, whole genome shotgun sequence.
ACCESSION   DAOREF010000249 DAOREF010000000
VERSION     DAOREF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17766_length_3402_cov_2.570362"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKI33_06365"
     CDS             <1..261
                     /locus_tag="PKI33_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36664.1"
                     /translation="VTREDLQNLTLSLCAEQNITLVIVTHSIEEAAVLGKKILLLSNP
                     PNQTVQVFDNPNAGTTGFRHTPEFHSMCEHLWREMQKGNVSL"
     gene            258..998
                     /locus_tag="PKI33_06370"
     CDS             258..998
                     /locus_tag="PKI33_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36665.1"
                     /translation="MKRRDVLFAALAILLVWQLAAMIVNMPILPSPLTVCMVFFKEMQ
                     NGLYIHFAVSLWRVTAGMLLSVLVAAPAGLLIGGSKSLNRLLSPIIYLLYPIPKVVFV
                     PVIILFLGIGDTSKIVIMFLILFFQIIVLVRDQASGIAPQLLQSLQSLGAGRRALFRF
                     VYLPASLPAILTALRQSIGTAVAVLYITELSATKYGLGYYIYYKGSTLLDYPSMYAGV
                     IAMSLLGLGMYFTVDWLERKWCPWKFVS"
     gene            complement(991..1890)
                     /locus_tag="PKI33_06375"
     CDS             complement(991..1890)
                     /locus_tag="PKI33_06375"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNM36666.1"
                     /translation="MTETNKPNMFQIWWLAIRPRTLPAAAAGVAMGSALAWHDGSFQL
                     LPALAALLVALLLQVGSNVANDVFDFERGADTAERLGPVRVTQAGYLTPSQVKTGMVV
                     IFAFAAVLGLYLATLRGWTVIWLGAAAILSAVAYTGGPYPLGYHGLGDVFVFLFFGLA
                     AVAGTYFVQVGSVSPAAWWMAVPVGLIVTAILVVNNLRDLENDRKAGKRTLAVRFGEE
                     GAKAEYLLLMLIAYLIVPVLVWIKIIPIGALLAWLSLPVAIRTLRIVLTQKGKPLNAA
                     LAGTGQTAFLFSLFFWIGLLFSN"
     gene            complement(1898..3160)
                     /locus_tag="PKI33_06380"
     CDS             complement(1898..3160)
                     /locus_tag="PKI33_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNM36667.1"
                     /translation="MSTTSYPFARESQLALDQARRIAEGNKLPFVDSLMLLLTLLQLK
                     NSRVQALLKSLNVRVDNLAARIVATIKLEAKEILQVSEGKPVLSSECETVLALASEEM
                     QLAGQSSISEFSLLLGMLKNTESKAGQILSQYAVVVDQAREKIKVFKETPVSQSNILR
                     KDGFLRRAFRNGISPIFISLVLFTIAMAAFLWLGIGNNPRAFLIAFIIGGWIISVALH
                     EFGHAITAFWGGDESVEDKGYLTLNPLKYSNPVLSVIIPVMMLLLGGIGFPGGAVYIN
                     IFALRKPKYRSLVSAAGPLANLLVAILLAIPFANFPFFFFFIYAPQKFLMALAFLAFL
                     QITSIIINLLPIPGLDGFGILEPFLPREIVGIASFIRPFGFLILYLFVFIDSPISNMF
                     WQSIWAITNLLSPSLAYYANEGLKLFLN"
     gene            3221..>3402
                     /locus_tag="PKI33_06385"
     CDS             3221..>3402
                     /locus_tag="PKI33_06385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36668.1"
                     /translation="MTQLTGQERAQYVQSMFSKIAHRYDLMNRLMTGGQDIRWRREVI
                     SLTKIRPHGSILDLGTG"
BASE COUNT          985 a          820 c          784 g          813 t
ORIGIN      
        1 gttactcgcg aagaccttca aaacttaacg ctctcattat gcgcagagca gaatataacc
       61 ttggtaattg tgactcattc tattgaagaa gcagcagtgc ttggaaaaaa gatattattg
      121 ctaagcaacc cgcccaacca aactgtgcaa gtatttgata accccaatgc tggaacgact
      181 ggtttccgtc atacgccaga atttcatagc atgtgcgaac acctttggcg tgaaatgcaa
      241 aaaggaaatg tctctctatg aaacggcgcg acgttctttt tgccgcacta gcgattcttt
      301 tggtatggca gctcgcagct atgatcgtga acatgcctat tttgcctagt ccgttaaccg
      361 tctgtatggt cttcttcaaa gagatgcaaa acgggttgta tatccatttt gcggtgagtt
      421 tatggcgcgt aacagcagga atgctgctct cggtcttggt ggcggctcca gcagggttgt
      481 taattggcgg gtcgaagagt ttgaaccgtt tgctctcccc cattatttat ttgctgtacc
      541 caattcctaa agtggtcttt gtaccggtta ttattttgtt cttagggatc ggcgatacgt
      601 ccaagatcgt cattatgttc ttgattctgt tctttcagat cattgtactt gtccgcgatc
      661 aggcaagtgg tattgcgccg caattgcttc aaagcctgca aagtctcggt gcgggcaggc
      721 gcgcgctctt ccgtttcgtt tatttacccg ctagtttgcc agccattcta actgcactac
      781 gccaatccat tggcacggcg gtggcggtgt tgtacatcac agaactttca gcaacaaaat
      841 atgggttggg atattacatc tattacaaag gtagcacgtt actggattac ccgtcgatgt
      901 atgcgggggt gatcgccatg agcctgttgg gacttggcat gtacttcacg gttgattggt
      961 tggaacggaa gtggtgcccg tggaaatttg ttagttagaa aacagcaacc caatccaaaa
     1021 gaagagactg aataagaaag cggtttgccc agtacctgcc agagcagcgt tgagcggctt
     1081 tcctttttga gtcaaaacta ttctcaatgt acggatggca acagggagag agagccaagc
     1141 aagcaaagct cctattggaa tgatctttat ccacaccaaa actggcacga tcaaataagc
     1201 aatcaacatc aagagcagat attctgcctt ggcgccctct tctccaaagc ggacagctaa
     1261 tgtgcgtttg cctgctttac ggtcgttttc gaggtcgcgc aaattgttga cgaccaaaat
     1321 cgcagtcacg atcaacccaa ccggaactgc catccaccaa gcggcagggc tgacggagcc
     1381 aacttgaaca aaatatgtcc ctgctacagc agcgagaccg aaaaagagaa agacaaaaac
     1441 atcgcccaag ccatgatagc caagagggta aggtccgccg gtgtaggcaa cagcggagag
     1501 aatggcagca gcgccgagcc agatcacggt ccagccgcga agcgtggcga gatataaacc
     1561 caatactgct gcaaaagcaa aaatcaccac catgcccgtc ttcacttgcg atggagtcag
     1621 atatccagcc tgagtcacac gcacgggacc aagccgttca gccgtatcgg caccgcgttc
     1681 aaagtcaaac acatcattag caacattgct accgacctgc aacaacaatg ccaccaacaa
     1741 agccgccaac gcgggcaaca attgaaatga accgtcatgc catgccaaag cactgcccat
     1801 tgcaactcct gccgccgccg caggcaaggt gcgcggacgg atggcgagcc accaaatttg
     1861 aaacatgttc ggtttgtttg tttcagtcat aacgttgtta attcaaaaag agtttcaaac
     1921 cctcgttggc ataataggca aggctcgggc tgagtaaatt tgtaattgcc caaatacttt
     1981 gccagaacat atttgaaatc ggcgaatcga tgaacacaaa gagatacaaa atcaaaaagc
     2041 caaatgggcg aataaaactg gcaatgccaa cgatttcccg tggtaagaac ggctcaagaa
     2101 taccgaagcc atccaagccg gggatgggca atagattgat gatgatagac gtaatctgca
     2161 aaaatgccag aaaagccagt gccatcaaaa acttttgagg ggcataaata aagaagaaaa
     2221 aaaatgggaa gttagcgaat gggatcgcta acaatatagc gaccaataaa tttgctaaag
     2281 gtccagccgc agagaccaag ctgcggtatt ttggtttgcg caatgcgaag atattgatat
     2341 acactgctcc acccggaaaa ccaatcccac ctaatagcaa catcataact ggaatgataa
     2401 cgctcaacac cgggttcgaa tatttaagcg ggttgagggt gagatagcct ttatcttcaa
     2461 cactttcatc gccaccccaa aaggctgtga tggcgtgccc aaactcatgc aatgccacag
     2521 agataatcca cccgccgata atgaacgcaa tcaagaaagc tctgggatta ttcccaatgc
     2581 ccagccacaa gaaagctgcc atggcaatcg taaacaacac caaactaata aagatcgggc
     2641 taatgccatt tcgaaacgct ctgcgcagaa acccatcttt gcgcaaaata ttactttgtg
     2701 aaacaggggt ttctttgaat accttaattt tctcacgggc ttggtctact acaaccgcat
     2761 attgagataa tatttgcccg gctttgcttt ctgtgttttt taacatgccc aacaaaaggc
     2821 taaactcact gatcgagctt tgacctgcca gttgcatctc ttcagaagcc aatgctaaaa
     2881 cagtttcaca ttcagaagat aagaccggtt tcccctcaga aacttggagg atctctttcg
     2941 cctccagctt gatagttgcc acgatgcgcg cagcaaggtt atccactctg acattgagag
     3001 atttcaacaa ggcttgcaca cgtgaatttt tgagttgcaa cagagtgagc aaaagcatca
     3061 aagaatctac aaaaggaagt ttattcccct ctgcaatgcg gcgagcttgg tcaagcgcaa
     3121 gctggctctc ccgggcaaat ggatacgatg tagttgacat tttgcgatta taccgtgcgt
     3181 caaaaacctt ctgtgtataa ttcgtttttc aggagaattt atgacccaat taaccgggca
     3241 ggaacgtgcc cagtatgtac aatccatgtt cagtaagatc gcccatcgct acgatctgat
     3301 gaaccgactg atgacaggcg ggcaagacat ccgttggcgg agagaagtca tcagcctgac
     3361 aaagatccgc ccgcacggct ctattctgga cctcggaacg gg
//
LOCUS       DAOREF010000250         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17826_length_3396_cov_4.357677, whole genome shotgun sequence.
ACCESSION   DAOREF010000250 DAOREF010000000
VERSION     DAOREF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17826_length_3396_cov_4.357677"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKI33_06390"
     CDS             <1..489
                     /locus_tag="PKI33_06390"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM36669.1"
                     /translation="NESLLTKLIEHNNWANLQIILACSALNDKQLDAKPQSTTKGSIR
                     STLLHLVASQRSYLSMLTQAEEAQSQTPLTFAELQASAEISGAGLLAVARDEATMSVK
                     TKIQAKDGFIIVPWVIMVQVINHATEHREQIKSMLSDLGVLPPKIDGWSYGEFTNAYT
                     ER"
     gene            503..988
                     /locus_tag="PKI33_06395"
     CDS             503..988
                     /locus_tag="PKI33_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36670.1"
                     /translation="MTNELLLRDVSLDDLPIFFEQQRDEEANTMAAFTAKDPNDREAF
                     FAHWQRILADETVINKTIVVHGQVVGSVASYIDEGKPEVTYWLGKEYWGKGIATKALR
                     EFLAHHNQVRPIYARVAKDNLGSRRVLEKCGFTVIGESQGFANARGQEIEELLLERSA
                     A"
     gene            1052..2731
                     /locus_tag="PKI33_06400"
     CDS             1052..2731
                     /locus_tag="PKI33_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNM36671.1"
                     /translation="MANPEHFEVLKQGMEKWNTLRSKNFDTVFDLSGADLSGMNLVGI
                     RFYNVNLSGANLSNTYLRNADFSSSDLSGANLTGADLSQAYMFGVKLSDANLEGAIFE
                     GVNADNEVLKLISQSTANPEHLKILKQGVSAWNAWRQENPRVTPILSNADLHELTDMT
                     RVNLSGVIMHGVNLAGVNLESAEFNHANLSEANLAGVNLRTASLTGANLSKANLHGAN
                     LRGVSLIQADLSEADLSNANLDVIPDRDGDTEAFLSYATFRATNLSGASMRKAILRYS
                     NLVDANLTGANLTDADLYNADLVRSNLTNATLTGSDLSLADLVETNLTNAEISGCRIY
                     GASAWNVTLENTSQKDMVISRKDEPVVTVDDLEVGQFIYLMLNNTKIRDVINTITSKG
                     VLILGRFADPQRKAVLDGLREKLREFDLLPIVFDFDRPTDKDYTETVQTLAGMSMFVI
                     ADVTSPKSTPLELEATAKQFKIPFIPIIDISVDPRPFAMLVDLQKSFHWVLPTLQYQT
                     KEDLLDDENLKTYIIDRAVAKREELRAAKSQEPEMLLVRKKGTSTPPTTAS"
     gene            2758..3039
                     /locus_tag="PKI33_06405"
     CDS             2758..3039
                     /locus_tag="PKI33_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNM36672.1"
                     /translation="MSFQAYLDKVEELTGKAPKEFVAEAKKKKLTTHKEILDWLKTDY
                     GLGTGHARAIAHVILHGAEFTVKQTTGTHRDASGTLNLNGKKAAKKKKS"
     gene            3067..>3396
                     /locus_tag="PKI33_06410"
     CDS             3067..>3396
                     /locus_tag="PKI33_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36673.1"
                     /translation="MPKQRDSRFITIRRGGTLQDSDHHLLALWAADCAEHVLHLFENI
                     CPQDDRPRQAIESARAWTRGEISMSQSRAAGGHAMAAARALSGAARHAAFAAGQAGVV
                     AHVAAHEL"
BASE COUNT          986 a          816 c          819 g          775 t
ORIGIN      
        1 aacgagagtc ttcttacaaa gttaatagaa cacaacaatt gggcaaacct ccaaatcatt
       61 ctggcttgtt ctgctttgaa tgacaaacaa ctggacgcta agcctcagtc tacgaccaag
      121 ggcagtattc gtagcacctt gttacatctt gtcgcctctc aacgaagcta tttatccatg
      181 ttgacacagg cagaggaagc acaatctcaa actccgctca catttgctga gttgcaagcg
      241 tctgcagaaa taagcggcgc aggcttgcta gcagtggcaa gagatgaagc aaccatgtct
      301 gtaaaaacaa aaatacaggc aaaggatggc ttcatcattg tgccttgggt gattatggta
      361 caggttatca atcacgccac tgaacaccgg gagcagatca aaagcatgct tagcgatttg
      421 ggtgtattac ctccaaagat cgatgggtgg agctatggcg aatttacgaa tgcatacaca
      481 gaaagatagg caaatttacg acatgacaaa cgaacttctt ctgcgggatg ttagcctcga
      541 tgaccttcca atcttttttg aacaacaacg agatgaggaa gccaatacca tggcggcatt
      601 taccgccaaa gacccaaatg atcgagaagc ctttttcgcc cactggcaaa gaattttggc
      661 agatgagacg gtcatcaata agaccatcgt tgtccacggg caggttgtag gcagtgttgc
      721 cagttacata gacgaaggca aacctgaagt cacctattgg cttgggaagg aatattgggg
      781 caaaggcatt gcgacaaaag cattgagaga gtttttagcc caccacaacc aagtacgccc
      841 catctatgcg cgtgtggcaa aagataacct cggctcgcgg cgcgtattgg aaaaatgcgg
      901 ctttacggtc atcggagaat cccaagggtt tgccaacgca cgcggtcagg agatcgaaga
      961 gttgctttta gaacgatcag cggcgtaagc tcatcaaaac tgatatcatc gagaaaaaag
     1021 caaccacttt aaaaagagga gcctatccct tatggcaaac cccgaacatt tcgaagttct
     1081 aaagcaaggc atggagaaat ggaatacact ccgaagcaaa aattttgata cggtctttga
     1141 tcttagcggc gcagacttga gcggtatgaa tttggttggc atacgtttct acaatgttaa
     1201 tttaagcgga gccaacctat ccaataccta tttgcgcaat gcagatttct catcttcaga
     1261 ccttagcgga gccaatctca ccggtgcaga tttaagccaa gcctatatgt ttggggtgaa
     1321 gctgagtgac gcaaatttag aaggtgcgat ttttgaaggg gtgaatgcag ataatgaagt
     1381 tcttaaactc atcagtcaat caacagccaa ccccgaacat ctgaagattc ttaagcaagg
     1441 ggttagcgca tggaatgcct ggcggcaaga aaaccccaga gtgaccccca ttctgagcaa
     1501 tgctgacctg cacgagttga ccgacatgac acgcgtcaac ttgagcggcg tcataatgca
     1561 cggcgttaac ctcgccgggg tcaacttgga atcggcagag tttaaccatg ccaacttaag
     1621 cgaagcgaac cttgccggag tcaacttacg aaccgcatcc ttaaccggag cgaacctgag
     1681 caaagcaaat ttacatgggg caaatttgcg aggcgtgtcc ttaatccagg cagacctcag
     1741 cgaagcggat ttgagcaacg ccaatttgga tgtcatccca gatcgtgatg gcgatacgga
     1801 ggcgttccta tcttatgcta cgttcagggc aaccaacctt agcggagcca gcatgcgcaa
     1861 agccattttg cgctactcaa acttagtgga tgcaaactta accggagcca acctgaccga
     1921 tgccgacctc tataatgccg atttggtccg gtcgaacctc accaacgcga cactcaccgg
     1981 ctctgacctc agcctggctg atttggtgga aactaattta accaacgctg agatctcagg
     2041 ttgtcgaatt tatggggctt cagcatggaa tgtcactttg gagaatacct ctcaaaaaga
     2101 catggtcatt tcgcggaaag atgaaccggt tgtcactgta gatgatctgg aagttggtca
     2161 gttcatttac ctgatgctga acaataccaa gatccgagat gtgatcaata ccatcacatc
     2221 gaaaggtgta ctcattcttg gcaggttcgc cgacccgcag cgcaaagctg ttttggatgg
     2281 actgcgagaa aagctgcgcg agttcgacct gttgccgatc gtcttcgatt ttgaccgccc
     2341 caccgataaa gactataccg aaacggttca aaccctggca ggtatgagca tgttcgtcat
     2401 cgccgatgtg accagcccga agtcaacccc gttggagttg gaagccaccg ccaaacaatt
     2461 caaaattcct tttattccta ttatcgatat cagcgtagac ccacgtccat ttgccatgtt
     2521 ggttgatttg caaaaatctt ttcattgggt tttgccaacc cttcaatatc aaaccaaaga
     2581 agacctgttg gatgatgaga atctaaaaac ctatatcatt gatcgcgccg ttgcaaaacg
     2641 tgaagagtta cgagccgcaa aaagccagga acccgaaatg ctgctcgttc gtaaaaaagg
     2701 cacgtccacc ccacctacaa cagcaagttg aggcgttacc ccttacagga gaaaaccatg
     2761 tcgttccaag cttatttaga taaagttgaa gaacttacag gcaaagcccc gaaagaattc
     2821 gtggctgagg caaagaaaaa gaagctgaca acacataaag agattctcga ttggttaaaa
     2881 acggattacg gtctgggtac cgggcatgcc agagccattg cacatgtgat tttgcacggt
     2941 gctgaattta ccgtaaaaca aaccaccgga acgcatcgcg atgcctctgg cacgcttaac
     3001 ttgaatggca aaaaagccgc taaaaagaaa aagtcttaaa caacggcatt aaaccaagcc
     3061 atgatattgc ctaaacaacg agactctaga ttcatcacca tacggcgcgg cggcacctta
     3121 caagattcag accatcacct attggcatta tgggcagcag attgtgctga gcatgtcttg
     3181 cacttgtttg agaatatttg cccacaagac gaccgtccgc gccaggcaat tgagtctgct
     3241 cgggcatgga cgcgtggtga gatcagcatg tcacaaagcc gggcggcagg cggacatgcc
     3301 atggctgcgg cgcgtgcctt gagtggtgcg gctcggcatg ccgcttttgc cgcaggtcaa
     3361 gccggggtgg tggcgcatgt agctgcccat gaactg
//
LOCUS       DAOREF010000251         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17827_length_3396_cov_3.972164, whole genome shotgun sequence.
ACCESSION   DAOREF010000251 DAOREF010000000
VERSION     DAOREF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17827_length_3396_cov_3.972164"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKI33_06415"
     CDS             <1..278
                     /locus_tag="PKI33_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36674.1"
                     /translation="IEYIYEECPFAEGATQIFYKETLNQLENTRPGIKLTFYLRFLEA
                     RQSGELFIQREETQPHIHPCEKCGQPTSAPGLCSFCRMLEKADVVTE"
     gene            complement(285..1274)
                     /locus_tag="PKI33_06420"
     CDS             complement(285..1274)
                     /locus_tag="PKI33_06420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM36675.1"
                     /translation="MPQSLLNGEILRERYVVREQIGQGGTGNIYLADDVRLQGRLCAI
                     KEVEHNQVLPPDVLAQAREQFFREASVLARLDHPNLPKVSDFFSEETRDYLVMDYVPG
                     KDLRQLMQEARRSNTFIPEREVLSWANQIAEALNYLHHQDPPIVHRDIKPSNIKLTPS
                     GLVKLVDFGLVKIMAPGEVTITVIQGQGTALYTPLEQYGGDDTHTDAQADIFSFGATL
                     YHLLTNEPPAEARKRFLNSRSLTPPIDINPRISSHVEKAIFWAMALHPNDRPVDVLTF
                     RDALFGKIEIPIQVDVERYGFETPRFSVFSTEQIAGYVAFGLFLVGLLATVIR"
     gene            1463..2119
                     /locus_tag="PKI33_06425"
     CDS             1463..2119
                     /locus_tag="PKI33_06425"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNM36676.1"
                     /translation="MEREEEYPILVAQDGPLKGQRWTLSKTLMMGRDPSCDIQVQDRQ
                     VSRFHARITPTQEGVTLEDLGSKNGTNHNGTEIAGPIMLQDGDAFGIALAQQFTFLTS
                     DATMPLAESGPRSGRLVMEQKSRQVWVNQQQLIPPLSAQQFKLLWTLYENQGQVIERT
                     SLVSDVWGEEQTAGVSDQALDALIRRLRDRLAVLDPSHQYINTVRGHGLRLDNPPIGE
                     "
     gene            2127..2600
                     /locus_tag="PKI33_06430"
     CDS             2127..2600
                     /locus_tag="PKI33_06430"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNM36677.1"
                     /translation="MDAQSEKVLAQVIREARIASLGTLHDEAPQVSMVAYVYAEDFSA
                     FYIFVSRLAQHTVDMQKDNRVSLMICETDDARSDPQTLVRVSIRGKAEMLQTGEPGYT
                     PLKEQYIARFPESEKLFNLSDFSFWRISPRGGRYVAGFAKAFNITADTLQKAALR"
     gene            2652..>3396
                     /locus_tag="PKI33_06435"
     CDS             2652..>3396
                     /locus_tag="PKI33_06435"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNM36678.1"
                     /translation="MTQLKLLAIFAHPDDEAMGMGGTLAKYAAEGVETHLVCASRGEV
                     GWFGPEEQNPGPERLGQLRTEELQNAVDTLGMKSLSFLNYIDGQVDQAEPTEAIAKIV
                     THIRRIQPQVVVTFPHDGNYGHPDHIAIGQFTNAAIVCAADSSYKDTENLPAYRVSKL
                     YYMVDGESFINLIAPFMGDMTFPVDDQLRGENAWKEWMITTKIDMTEHCHTAWKAIMC
                     HKSQLATLGALAEMHEDAAVAVLAMQGTFF"
BASE COUNT          871 a          840 c          880 g          805 t
ORIGIN      
        1 gcattgaata catttatgag gaatgtccat tcgcggaagg ggcaactcag atcttttata
       61 aagagacgtt gaatcagctg gaaaatactc gcccgggcat caagctaaca ttttatttac
      121 gttttctgga agcaaggcag agcggagagt tgttcattca acgagaagag acccagccgc
      181 atattcaccc atgtgaaaaa tgcggtcagc cgacctctgc gccggggctt tgttcgttct
      241 gtagaatgtt ggaaaaggca gacgttgtaa cagaatagac cttgctaccg aatgacggtg
      301 gcaagtaacc ccacaagaaa tagaccgaag gctacatacc ccgcgatctg ctctgtagaa
      361 aaaacggaga agcgcggggt ttcgaatccg tagcgttcta catcgacctg aatcggaatc
      421 tcgatctttc caaacaacgc atcgcggaag gtgagcacat caacagggcg gtcattgggg
      481 tgtaatgcca ttgcccagaa gatggctttt tccacatgcg agctgatgcg tggattaatg
      541 tcaatgggtg gagtcaggct gcggctattt aagaagcgtt tgcgcgcctc agcgggcggc
      601 tcattcgtca aaagatgata gagagtcgcg ccaaaggaga aaatatccgc ttgtgcatcg
      661 gtgtgcgtat catcccctcc atattgttcg agcggcgtgt agagtgcagt gccttgccct
      721 tgaatgacgg tgatggtcac ttcgccaggc gccatgattt taaccaaacc aaaatcgacg
      781 agtttaacca agccactagg agtcagcttg atattgctag gcttgatatc acgatgcacg
      841 atcggcgggt cttgatgatg taagtaattg agcgcctcag cgatctgatt tgcccaactg
      901 agcacttctc gttctgggat gaaggtattg cttcggcgtg cttcctgcat tagttgacgc
      961 aggtctttgc cggggacgta atccatcaca agatagtcac gagtttcttc ggagaagaag
     1021 tctgaaactt tggggaggtt ggggtggtcg aggcgtgcga gaacagaagc ctcgcgaaag
     1081 aattgttcgc gggcttgtgc aagcacgtcg gggggaagaa cctgattgtg ttctacttct
     1141 ttgatggcac acagacgccc ttgtagacga acatcgtctg caaggtaaat attgcccgtg
     1201 ccgccctgcc cgatctgttc acggacaacg tagcgttcgc gcaggatctc cccgttcaat
     1261 aatgattggg gcaaaacttc tccttcttgg ttgaaagcgg ctgcaagagt tgagtgatat
     1321 tttcggcgtg tatcactttt gcaatcccga aaaggggatt tgcaatatat aatcgtgcac
     1381 agaaaccact tttctgctat attataaacg cctttgcaat ggatagcaca attaaatcta
     1441 taacaaaggc gatgaggaga ttatggaacg cgaagaggaa tatcccattc ttgttgcaca
     1501 agatggtccc ttgaaggggc agcgttggac gttaagcaag actttaatga tggggcgtga
     1561 cccaagttgt gacattcagg tgcaagaccg tcaagtttct cgctttcacg cccgcattac
     1621 acccacgcaa gaaggggtga cgctcgaaga ccttggcagc aagaacggaa cgaatcacaa
     1681 tggaaccgag atcgctggtc cgatcatgct acaggacggc gatgcattcg gaattgcact
     1741 ggcacaacaa ttcacgtttc taacttcaga tgccaccatg ccactcgccg aaagcggacc
     1801 gcgctctggg cgcttggtga tggagcagaa gtcacgtcag gtgtgggtca atcaacaaca
     1861 gttgatcccg ccgctttctg cgcaacaatt taaactgttg tggaccctgt atgaaaatca
     1921 aggtcaggtg atcgaacgca ccagcttggt ttctgatgtt tggggtgaag agcaaactgc
     1981 aggcgtttca gaccaggcgt tggatgcatt gatccgccgc ttgcgcgacc ggcttgccgt
     2041 gctcgacccc agccatcaat acatcaacac cgtccgcgga catggtctgc ggttagataa
     2101 cccaccgatc ggcgaataaa tttacaatgg acgcacaatc agaaaaagta ctcgctcaag
     2161 ttatacgtga agctcgcatt gcttcgcttg gaaccttgca cgacgaagcc ccgcaagtct
     2221 ctatggtcgc gtatgtgtat gctgaagatt tttcggcatt ctatattttc gtcagccgct
     2281 tggcgcaaca taccgtggat atgcaaaaag ataatcgcgt gagtctcatg atctgcgaaa
     2341 cagacgatgc tcgcagcgac ccacaaacct tggtacgcgt gtccattcgt ggcaaggcag
     2401 agatgcttca aaccggtgag ccgggctaca ctccgctcaa agaacagtac attgcgcgtt
     2461 tccccgagtc cgaaaaatta ttcaaccttt ctgattttag cttttggcgt atctctccgc
     2521 gtggcggacg ctatgtggca ggttttgcca aagcattcaa catcacagcc gacaccctgc
     2581 aaaaagcagc ccttcgataa cctcattgac agtcactccg gtggctgtca gtttttttag
     2641 gagcaatcaa catgacacaa cttaaactgc tggcgatctt tgcacacccc gatgatgaag
     2701 ctatgggcat gggcgggacg ttggcgaagt atgccgccga aggcgtggag actcatctcg
     2761 tctgcgcttc acgcggagag gtgggctggt tcggccccga agaacagaat ccaggtcccg
     2821 agcgactcgg gcaactccgc accgaagagc ttcagaacgc cgtcgacaca ttaggcatga
     2881 aaagcctttc ttttttgaat tacatcgatg gtcaagtgga ccaagccgag ccgactgaag
     2941 ccattgcaaa gatcgttaca catattcgtc gcatccaacc gcaagtggtg gtgaccttcc
     3001 cgcatgatgg taattacggt catcccgacc acatcgctat cggacagttc accaatgctg
     3061 ccatcgtctg cgccgcggac tcaagctaca aagacactga gaatcttcct gcttaccgtg
     3121 tctctaaact ttattacatg gtagacggtg aaagtttcat taacttgatc gcgcccttta
     3181 tgggagatat gaccttcccg gtcgatgacc aactccgtgg cgaaaatgcc tggaaagaat
     3241 ggatgatcac caccaagatc gatatgacag aacactgtca caccgcttgg aaggcgatca
     3301 tgtgtcataa aagccaactt gctaccttgg gcgcccttgc tgaaatgcac gaagatgcag
     3361 ccgtggcagt ccttgccatg cagggaactt tcttcc
//
LOCUS       DAOREF010000252         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17863_length_3392_cov_3.528319, whole genome shotgun sequence.
ACCESSION   DAOREF010000252 DAOREF010000000
VERSION     DAOREF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17863_length_3392_cov_3.528319"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKI33_06440"
     CDS             complement(<1..550)
                     /locus_tag="PKI33_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNM36679.1"
                     /translation="MINRRDFLKAAGMGIAATALAACAEPAPAAEASASASDLPELDW
                     QMATSWTQGLDVLFGTTQAFADRVAALTGGKFKITPRAGGELAKATEVLDVVSSGAVP
                     IGHTASYYYIGKSWVMAFGTTLPFGLTAAQQKAWLYSGGGLELLQAFYAKTFNVIQFP
                     AGNTSAQMGGWFRKEINTVADLQ"
     gene            complement(594..2237)
                     /locus_tag="PKI33_06445"
     CDS             complement(594..2237)
                     /locus_tag="PKI33_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNM36680.1"
                     /translation="MIQIQLSNITLVLGAKRIFENLNWEIQRGQRIGLIGANGAGKSS
                     LFKLIEGEHAPELGGSITRARLITTGYLPQHPQLDPRLTALDAAMQGNPRVGEVHAEL
                     ERVEASLGDPEVYGNEKKLERALERQHQLIEEYHVLGGDSYPERVKGILLGLGLAQSE
                     LTKPLSVLSGGQKKLVGLARLLLLNPDILLLDEPDNHLDLPGKMYLEKLIREYDGTVV
                     IISHDRYLLDAAVTHIAELEDGKMTVFEGDYTSYMADKEMRLARQEEMFRAQQVTIKR
                     LEAAIKRLAIWGKVYDNEDLARKSKTMQKRLDKMDKVEKPVTERRRMEMQLNGWRGSN
                     KVLELADISKSFDEKRVFANVNETIWHGERVGLIGANGAGKSVLLRMILGKEMPDVGE
                     IKIGPSVSIGHYAQEHETLDFNQTVLDAVRYAGEMSESKATAFLLRYLFTYKQVSQKI
                     GELSGGERSRLQLALVVLSGANFLLLDEPTNNLDIASAEVLEQALEDFEGTVLVISHD
                     RYFLDQTMQRLLVIENGQLAGYAGGYSDYLAARNPKSTS"
     gene            complement(2476..>3392)
                     /locus_tag="PKI33_06450"
     CDS             complement(2476..>3392)
                     /locus_tag="PKI33_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNM36681.1"
                     /translation="ELIHVNSKRGKNAADIRIVLDAMELFYNGRDSFTHVLLASGDGD
                     FTELVHRLRANGKTVIGLGISGTTADYLVSACDKFVYYDKWQGVGKFKKETPSNGGQK
                     QQPVKQSTPPAQKQEKHPPQEKPQVQEKAQPQEKSAPKPATPISPEKRLERYLNILSS
                     HKIRMVPSTNRPLVIYKIYDIVKEDAGITFNQLKEKAQAVFANTKVEPQLALDTAHQL
                     FHTFCFEFDQSNDRIMDRKMLIPEDTKSPADLLNKCDQKLLHLIAGDLGASEVFDKEI
                     AAKILYGGVRNPKVLDHIQELLDAETQV"
BASE COUNT          787 a          902 c          831 g          872 t
ORIGIN      
        1 cctgcaagtc tgctacggtg ttgatctctt tacggaacca gccacccatt tgcgcgctgg
       61 tgttgcccgc cgggaattga atgacattga aggtcttggc ataaaaggct tgcaggagtt
      121 caagtccgcc gccggaatac agccaggcct tctgttgagc agcggtcaga ccaaacggca
      181 aggtcgtgcc gaacgccatc acccaactct tgccaatgta ataatacgaa gccgtatgcc
      241 caatcggtac cgcacccgaa gaaaccacat ccaatacctc ggtggcttta gcgagttcgc
      301 cgccagcgcg cggggtgatc ttgaacttgc cacccgtcaa tgcagcgaca cggtctgcaa
      361 aggcttgtgt ggtgccgaag agtacatcta agccttgcgt ccaactggtt gccatctgcc
      421 aatcaagttc gggcaaatcg ctggcagaag cggatgcttc cgcagcgggg gcaggctcgg
      481 cgcaagcagc caatgcagtg gcagcaatgc ccatacctgc ggctttcaaa aagtcacgtc
      541 ggttaatcat tctctctctc cttcgatggt ttttattatt ataagtttgt tgctcaactc
      601 gttgactttg ggtttcgcgc cgccaagtaa tcgctgtatc cgcctgcata cccagcaagc
      661 tgaccattct cgatcactag caaacgctgc atggtttggt cgaggaagta acggtcatgc
      721 gaaatgacaa gcaccgtccc ctcgaaatct tcaagggctt gttccagcac ttcggcagaa
      781 gcgatgtcga gattattggt aggttcgtcc aacaatagaa aattcgcacc agagagaacg
      841 accaacgcca actgcaaacg gctgcgctcc ccgccggaga gttcaccgat cttctgcgag
      901 acctgtttat acgtaaaaag atagcgcaac aaaaacgccg tagcttttga ttcgctcatc
      961 tcgcccgcat agcggacagc gtctaacacg gtttgattga agtcgagcgt ctcatgttcc
     1021 tgtgcataat gcccaatgga aacgcttgga ccaattttga tctcacccac atctggcatc
     1081 tctttgccaa ggatcatccg caacagcaca gacttacccg ctccattggc gccgatcagc
     1141 cccacccgct caccatgcca aatggtttcg ttgacatttg caaacacacg tttctcatca
     1201 aacgatttgg aaatatccgc caactccaac actttattcg agccgcgcca gccattcaac
     1261 tgcatctcca tacggcgacg ctccgttaca ggcttctcga ccttgtccat tttatccagc
     1321 cgtttctgca tggtcttgga cttgcgcgca aggtcttcgt tatcataaac cttgccccaa
     1381 atggcaaggc gcttgatcgc cgcttcaaga cgtttgatgg tcacctgctg ggcgcggaac
     1441 atttcctcct gccgtgccag gcgcatttct ttatccgcca tgtacgaggt gtaatcgcct
     1501 tcgaaaacgg tcatcttgcc atcttcaagt tcggcgatat gcgtcacggc ggcatcaagc
     1561 aaataacggt catgtgaaat aatgacaact gtgccatcgt attctctgat cagtttttca
     1621 agatacatct tgccaggcag gtcgagatga ttatccggtt catccaacaa caagatatct
     1681 ggattcagta acagcagccg cgctaacccg actagttttt tctgcccgcc gctcaacacc
     1741 gagaggggtt tggtcaattc gctttgagcg agtcccagcc cgagcaggat tcccttaacc
     1801 cgttccgggt atgagtcacc gccgaggacg tgatactctt caatgagttg atgctgtcgt
     1861 tccagtgcgc gctccaactt cttttcattg ccatagactt cagggtcacc caaactggct
     1921 tcgactcttt caagctccgc atgcacctcc cctacacgcg ggttgccttg catcgcggcg
     1981 tcgagcgctg tcagccttgg gtcaagttgg ggatgttgag gcaggtatcc cgttgtaatg
     2041 aggcgggcgc gggtaatgct tccgcccagt tcgggggcat gttccccttc aataagtttg
     2101 aatagcgacg acttgcccgc gccattcgca ccgataagac caatacgctg tccgcgctgg
     2161 atctcccagt tgagattttc aaagatgcgc ttggcaccga ggacgagtgt gatgttggag
     2221 agttgaattt ggatcatagt tttttccggt ttgtaggggc gacccttcaa ggtttgtcaa
     2281 agtgtttgat caatctgggc gggttgccct tacgatatta ttaaacaaaa acgccgcagg
     2341 caatgcctgc ggcgtatgaa agtaaaaact ttcagcgtac aggcgcaata cgacaaggaa
     2401 cggcgttatc aaagccattg atcgccattg caaaaagggg tttgcctacg ctgatgatgt
     2461 aaagcatatt tgcgattata cctgagtttc tgcgtcgagc aattcttgaa tatgatcaag
     2521 caccttgggg ttgcgtacac cgccatacaa aatcttggca gcgatctctt tatcaaaaac
     2581 ttccgaagca ccaaggtcgc cagcaattaa gtgaagcaac ttctggtcac atttgttgag
     2641 caggtcagca ggtgattttg tatcttcagg aatcaacatc ttgcgatcca taatgcggtc
     2701 gttgctttgg tcaaactcaa aacagaaggt atggaaaagc tgatgcgccg tatccaacgc
     2761 gagttgcggc tcgacctttg tattcgcaaa aactgcttgt gctttttctt ttagttggtt
     2821 gaaggtaatg cccgcgtctt ctttgacgat atcgtaaatc ttataaatca ccagcggacg
     2881 gttcgtgctt ggcaccatgc ggatcttgtg cgaagaaaga atattgagat aacgctccag
     2941 gcgtttttct ggtgagatcg gtgtggcggg cttcggcgcg gacttctctt gaggttgcgc
     3001 cttttcttgg acttgaggct tctcctgcgg tggatgtttt tcctgttttt gcgcaggcgg
     3061 agtgctttgc ttaactggct gttgtttctg cccgccgttc gaaggcgttt ccttcttgaa
     3121 cttgcctaca ccctgccatt tatcgtagta cacgaacttg tcacaagcgc tgacgagata
     3181 atctgcagtg gtgccgctga tgcccaaacc aataacggtc ttaccattgg cgcgtaaacg
     3241 atggaccaac tctgtaaaat caccatctcc tgaagcaagc aaaacatgcg tgaaggaatc
     3301 acgtccgttg taaaaaagct ccatggcatc gagcacgatg cgaatatcgg cggcgttctt
     3361 gccgcgcttg ctgttgacgt ggatcaactc ta
//
LOCUS       DAOREF010000253         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17875_length_3391_cov_4.549161, whole genome shotgun sequence.
ACCESSION   DAOREF010000253 DAOREF010000000
VERSION     DAOREF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17875_length_3391_cov_4.549161"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PKI33_06455"
     CDS             <1..1025
                     /locus_tag="PKI33_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNM36682.1"
                     /translation="AAVRSTGFEELALLSLSSSDYTYVNDLVDAISKRFEGRKLTVSL
                     PSLRIESVSVDLMEKLKQHRSGGFTLAPEAASERMRRIINKYIPDEDIINTTRDIYAR
                     GWTTIKLYFMIGHPSETIEDVQAIADLCKRVLAEGRKVAGWKVKLHAGVSTFVPKPQT
                     PFQWVACDTRDNILEKQALLKREFFKDKSIKLSWTKPDDTLVEAWLSRGDRRMSEVIY
                     TAWKNGAKFDAWEEGRKFETWMAAFEENGIDHAFYTHRQRRTDEIFPWEHITAAVRKN
                     FLFQDFRMSLEGQIRVDCRLNCFACGILPTFANLRRENPGEGWKCPEVKTPARKVELE
                     LPIAGD"
     gene            1034..1561
                     /locus_tag="PKI33_06460"
     CDS             1034..1561
                     /locus_tag="PKI33_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36683.1"
                     /translation="MNKAILLERLQNERDQFELLLNRLGFARQLTMRGVVAGLTVKDL
                     VAEVFSHEQFIADRLSEILHGDTYAPSASFTALENFQREYGYPDYESSLIEKDKHDLS
                     VVDAYKHIEFDEIISQELAVYGNILESIEKLTHHEFLDHDLYNRIAEHTYRPYRRTSS
                     IINRWLKRIASETKK"
     gene            1564..2193
                     /locus_tag="PKI33_06465"
     CDS             1564..2193
                     /locus_tag="PKI33_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNM36684.1"
                     /translation="MRVRITFTKQGALRYIGHLDLHRVWERALRRASLPISYSQGFHP
                     QPKISLAAALPLGFSSRAEVIDVRLNEEIPTEEIHTRLKDNLPPDIKVTDVKSVDERL
                     PALQTLVLSAAYDVHLTEPVAGSELTRRVESLMQAESIIRERRGKTYDLRPLVEMLSV
                     ITQADGTVWLKMTLAAREGATGRPEEVLTVLEIEPETARVERTRLIFKE"
     gene            2329..3312
                     /locus_tag="PKI33_06470"
     CDS             2329..3312
                     /locus_tag="PKI33_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNM36685.1"
                     /translation="MALLAAIPMAFIPAFFFSWFLYWLDRYEKEPRWLLLMTFFWGGF
                     VAIIGTLIVASIFEVGFSFVLNDAVLEDIAGGSITAPLVEEFMKGFAVMLVFFMFRKE
                     FDSILDGIIYGGIAGLGFAATENVLYFLGQYNEAGWGGMFANFALRVGVFAWGHPFYT
                     AFTGLGFAVARNNKNILVKLIAPIIGYLLAVSAHAFHNTVLLFVSGLGSLAFVILVEW
                     GSWFIFLGFIAWMIRHEQGLLKKHLPEEVANGLISDAQYKTAVSFFQFGARMSALSGG
                     AYRATDHFYQYLGELAHKKEQLAKFGDERGNAAIIEKLRANIASLAAQAKT"
BASE COUNT          866 a          812 c          790 g          923 t
ORIGIN      
        1 atgctgcagt ccgctctacc gggtttgaag agctagcgct gctgtcgcta tcctcatcag
       61 actacactta tgtcaacgac cttgtggatg ccatcagcaa acggtttgaa ggtcgaaaat
      121 tgactgtctc gctgccttct ctgcgcatcg agtctgtttc ggtagattta atggaaaagc
      181 tcaaacagca tcgctcaggc gggtttactc ttgctcctga agctgccagt gaacgcatgc
      241 gccgtatcat caataagtac attcctgacg aagatatcat caataccact cgcgatattt
      301 acgcccgcgg ttggacgact atcaaactct acttcatgat cggtcacccc agtgaaacga
      361 ttgaagatgt acaagccatt gcagatctgt gcaaacgcgt ccttgctgaa ggacgtaaag
      421 tggcgggttg gaaagtgaag ttacatgctg gtgtcagcac ttttgtgcct aaaccacaaa
      481 cacctttcca atgggttgcc tgtgacacac gcgacaatat tcttgaaaag caagccttgc
      541 tcaaacgtga attcttcaag gataaaagca tcaaactaag ctggacaaag cctgacgaca
      601 ctcttgttga agcctggctt tctcgtggcg accgccgcat gtctgaggtg atctacacag
      661 catggaaaaa cggtgccaaa ttcgatgcct gggaagaagg gcgcaaattc gaaacgtgga
      721 tggctgcctt cgaagaaaat ggcattgacc atgccttcta tacccatcgt caacgccgta
      781 cggatgaaat cttcccatgg gaacatatca cagccgctgt tcgtaagaac ttcctcttcc
      841 aagattttcg tatgtcgctc gaaggtcaga tccgcgtgga ctgtcgcttg aattgtttcg
      901 cttgcggaat tctgcctacc tttgcgaacc tgcgccgcga aaaccccggt gaaggttgga
      961 aatgccccga agtgaaaacc cctgcccgta aagtagaatt ggagcttccc atcgctgggg
     1021 attagaaaca agcatgaaca aagccatact cctcgaacgc ttgcaaaatg aacgcgacca
     1081 gtttgaactt ttactgaatc gccttggttt tgcgcgtcaa ctaaccatgc gcggagtggt
     1141 agctgggctt actgtcaaag atcttgtagc agaagttttt tcacatgagc aattcattgc
     1201 agaccgttta agcgaaattc tgcatggtga tacctatgct ccgtcagctt ctttcacagc
     1261 cctcgaaaat tttcagcgag aatacggtta tcctgattac gagtcttcgt tgatcgaaaa
     1321 agataaacat gacctatctg tggtagatgc ttataaacat atcgaattcg atgaaatcat
     1381 ttctcaagaa ctggctgtat atggcaatat tctagagtcg attgaaaaac tgacacatca
     1441 tgaatttctc gaccacgacc tgtacaatcg catcgcggaa cacacttatc gcccctaccg
     1501 ccgtaccagt tccatcatca accgctggct aaaacgcatc gcctcagaga caaaaaaata
     1561 aatatgcgag ttcgaattac attcaccaaa caaggcgcat tgcgctatat tggtcatctt
     1621 gacctgcacc gagtatggga acgggcatta cgccgtgcca gtctgcccat ttcatactca
     1681 caaggctttc acccccaacc caagatcagc ctagctgctg cattgccgct tgggttttcc
     1741 tcccgtgctg aagtgataga cgtgcggtta aacgaagaaa tcccaaccga agaaatccat
     1801 actcggttga aggataatct tccgcccgat attaaagtga cagatgtgaa gtcagtggat
     1861 gaacgtttgc ccgctttgca aacactggtg ttatcagcgg cgtatgatgt ccatttgacg
     1921 gaaccagttg ccgggtccga actgacgcgt agggttgagt ctctcatgca agctgaatct
     1981 attatccgag aacgtcgtgg caaaacctat gatctgcgtc cgttggttga aatgctaagt
     2041 gtgatcacgc aggcagatgg caccgtttgg ttgaaaatga ctctagctgc tcgcgaaggc
     2101 gcaaccgggc gcccagaaga agtattgact gtgcttgaga ttgaaccgga aacggcgcgg
     2161 gtagagcgta ctcgcctaat tttcaaagaa taaacaaaac gtctggctta gccagacgtt
     2221 tgattttaga taagcgtttt gaaatacccc cttaagttct attgattttt agcccagcat
     2281 cagtaaaatt aatcagatat ctaatttcaa ctcactggag gaatttttat ggcactacta
     2341 gctgcaattc caatggcatt catcccagca ttctttttct cgtggttctt atattggctc
     2401 gaccgttacg aaaaggaacc gcgctggctg ctcctaatga cctttttctg gggtggcttt
     2461 gttgccatta tcggcacatt gatcgttgct tcgatttttg aggtcggttt cagctttgtg
     2521 ctgaacgacg ctgtccttga agatattgct ggcggctcca ttactgctcc attagtcgaa
     2581 gaattcatga agggctttgc cgtaatgctg gtcttcttca tgttccgcaa agagtttgac
     2641 tccatcctcg acggcattat ttatggtggc attgcagggc tcggctttgc tgcaactgaa
     2701 aatgttttgt attttctagg tcaatataat gaagcgggct ggggcggcat gttcgcaaac
     2761 tttgccttgc gtgtgggcgt gtttgcatgg ggtcacccat tctatactgc cttcaccggt
     2821 cttggttttg ctgtagcccg taataacaaa aacatcttgg tgaaacttat tgcaccaatc
     2881 attggttatc tgcttgctgt ttcggcgcat gcgttccaca atactgtttt gctcttcgtg
     2941 agcggattag gaagcctagc ctttgtcatt ttggttgaat ggggaagctg gttcatcttc
     3001 cttggcttca ttgcttggat gatacgtcat gaacaaggtt tattgaagaa gcatctacct
     3061 gaagaagtag caaatggatt aatttctgat gcacagtata aaacagccgt ctctttcttc
     3121 cagtttgggg cacggatgtc cgctctgagt ggcggcgctt atcgcgctac cgatcacttc
     3181 tatcaatacc ttggcgaatt ggctcacaaa aaagaacaac tggcaaaatt tggtgacgaa
     3241 cgtggaaacg ccgcaatcat agaaaagcta cgggcgaaca ttgccagcct tgccgcgcag
     3301 gcgaagacct aagacatttc gcttcatctc taaatgtagg gctggcggct gctccgtcag
     3361 cccttttcta cgcctatggt aaaattcgct t
//
LOCUS       DAOREF010000254         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17933_length_3385_cov_4.351351, whole genome shotgun sequence.
ACCESSION   DAOREF010000254 DAOREF010000000
VERSION     DAOREF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17933_length_3385_cov_4.351351"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKI33_06475"
     CDS             complement(<1..498)
                     /locus_tag="PKI33_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNM36686.1"
                     /translation="MPNFPVIGYLPDYRELNPAWASSLTDIIYFSAEPRADGSLDTSR
                     LTEETWQALASLKDENGIRVHISIGGWGRDGAFVEMSADPETRKTFIANLLNFLQEHQ
                     LDGADFDWEFPQNDTEFEQYIALLTETKAALSPHGLLVSVALPAESSFPLGEFAVVDR
                     VHLMSY"
     gene            658..1221
                     /locus_tag="PKI33_06480"
     CDS             658..1221
                     /locus_tag="PKI33_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36687.1"
                     /translation="MLKPTDLILVCLMPTPRDMEIARLLGWYRIPLKSAPKVVSVDYL
                     AFYQPTSFGERGGQVEYIAQVRGHELTTRGELLKDEADHPRAKEEYFKLQLGNLEKLK
                     EPVRTDKWKRLTFLYSTGEYLLNAKTLNDLVVEGEERNMLWKNLRERAENDQLYQVDL
                     PEADIPPEVLMALLGIKDLAGGYDAGQ"
     gene            1308..2978
                     /locus_tag="PKI33_06485"
     CDS             1308..2978
                     /locus_tag="PKI33_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNM36688.1"
                     /translation="MPADLMVVSPSSSLGEAIRRSLEDTKFYRVTTVNNKAAAIVKAN
                     EIGMPMAMLDLALGEEWVAEIGASLRTIRPSINLVILCEEAGSPPAFDSLRPWIMVRK
                     PFKISDFMNAISQPQSAQPAKSGAPAPAMPMSSTAQFMPWLNDPNKAAQHLTRLTLES
                     SAQAALITKKNDLWAYAGGLSQNAAKEVAQTVTRNWDGQKGSDLLRFIRLESTKAEHM
                     LYATRLTSDAVLALVFDAETPFSTIRSQASQLLSDFGSEKAEPKNQPAVSYAVSDDDS
                     KDEDLNIPPISNILADIPAPNPEPLTTRDFNLPRKTPVQPQPQEAFDPNQTRVSDALS
                     NASVFSRDPSPAVRRDSVPQPMAQTQKSEAHHDTQIDFDEVQVTAPSQPKARPETPVA
                     RPRPGENDATRESPSTEMARKLTAEPISAGLYHLTYACLLVPRFSSHYLTGDLSDRLG
                     EWLPNICIAFGWRLEFLAVRPEYLQWVVNVQPNTSPGHLMRIMRQQTSEKIFGDFPRM
                     KRENPSGDFWAPGYLIMGGSQPHPPQLVRDYIRQTRQRQGLEDLPPMK"
     gene            3085..>3385
                     /locus_tag="PKI33_06490"
     CDS             3085..>3385
                     /locus_tag="PKI33_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36689.1"
                     /translation="MPPTLYLIDGHALAYRMYFALTAGGGSQRWQTSKGEPTAGIYGF
                     ARELVRVLEQEKPEYLAVAFDVGKTFRDQIFPEYKATREKMPDDLRSQIVRIREMV"
BASE COUNT          850 a          917 c          857 g          761 t
ORIGIN      
        1 ataggacata agatgcacac gatcaacaac cgcaaactca ccaagcggga aactcgactc
       61 agccgggagg gcaacactca ccaacaagcc atgcggggaa agagctgctt tggtttcggt
      121 taataaggct atgtactgtt caaactctgt gtcgttttgt ggaaattccc aatcgaagtc
      181 ggcaccgtca agctgatgct cttgtagaaa gttcaacaag ttagcaataa aggttttacg
      241 agtttcaggg tcggcgctca tttctacaaa ggcaccatct cgcccccagc cgccaatgga
      301 gatatgtacg cgaatcccgt tttcatcctt taacgatgcc aatgcctgcc atgtctcttc
      361 tgttaagcgt gaagtatcca gtgagccatc ggcgcggggc tcggcagaga agtagatgat
      421 atcggtcaag gaagatgccc atgcggggtt cagctcgcga taatctggca aatagccgat
      481 gacggggaaa tttggcacag gcgctgaggt ggcggtcggt tggggcgtgg acgagggtat
      541 gggggttgaa tccacttcgg gcgcgggaag aggcgtagcc cctcctccac aagcaactag
      601 tagagaacat gatagaagga ggaatgagag tcgcatggtg aacattataa ttaagccatg
      661 ctcaaaccta cggacttgat ccttgtttgc ctgatgccta caccgcgcga tatggaaatt
      721 gcgcgtttgc tgggatggta tcgcatccca ttgaagagcg cgccgaaagt tgtcagtgtg
      781 gattatcttg cgttttacca acccacttcg ttcggcgaaa gaggcgggca agttgaatat
      841 atcgctcaag ttcgtgggca tgagttgaca accagaggcg aactgctcaa ggatgaagct
      901 gatcacccaa gagccaaaga agagtatttc aagttgcagt tgggaaattt ggagaagttg
      961 aaagagccgg ttcgaaccga taaatggaaa cggctgacct tcttgtattc cactggcgaa
     1021 tatttgctca acgccaaaac cttgaatgac ttggtggtag agggcgaaga gcggaacatg
     1081 ctttggaaga acctgcgcga gcgagccgaa aatgaccagc tttatcaagt ggatttgccc
     1141 gaggcagata ttccgccaga ggtgctgatg gcgcttttgg gcatcaaaga cctggcaggc
     1201 ggctacgacg cggggcagta aagttgtcca gaagagttac taaaatgata agaaaccacc
     1261 caaaggtttc cttgcatgcg tataacagaa gtgatacact ttaaccgatg cctgctgatt
     1321 taatggttgt ttccccttct agtagcctcg gtgaagccat tcgccgttcg ctggaagaca
     1381 ccaagttcta ccgagtcact accgtcaaca acaaggctgc agcgatcgtc aaggcaaatg
     1441 agatcggaat gccgatggcg atgctcgacc ttgcacttgg cgaagagtgg gttgcagaaa
     1501 ttggcgcatc tttacgcacc attcgcccca gcatcaacct tgttatcctc tgcgaagaag
     1561 caggttcccc gcctgccttt gattcattac gtccatggat catggttcgc aaaccgttca
     1621 aaatctctga ttttatgaat gccatcagtc aacctcaatc tgctcaacca gcaaagtctg
     1681 gcgctccggc tccagccatg ccgatgtcga gcacggcgca gttcatgccg tggctgaatg
     1741 accccaacaa agcggctcaa caccttaccc gtctcacctt ggaatcttcg gcgcaagcgg
     1801 cgctgatcac caagaaaaat gacttgtggg catatgcagg cggtctctct caaaacgccg
     1861 ccaaagaagt agcgcaaacc gtcacccgca attgggacgg acaaaaaggt tctgaccttc
     1921 tgcgctttat tcgactcgaa agcaccaaag cggagcacat gctctacgcc acgcgcctca
     1981 cttcagacgc cgtgctggct cttgttttcg acgccgaaac tccctttagc accatccgct
     2041 ctcaggcaag ccaattgctg agcgattttg gcagtgagaa agcggagccc aaaaatcaac
     2101 ccgccgtctc ctatgctgtc agcgatgatg atagcaagga tgaagactta aacatccccc
     2161 ccatctcgaa cattctcgcg gatattccgg ctccgaaccc tgagccactt accacacgcg
     2221 atttcaattt accgcgcaag acccctgtgc aacctcaacc gcaagaagca ttcgacccga
     2281 atcaaacccg cgtttcggat gcgctttcca acgcttcggt ctttagtcgc gacccatcgc
     2341 ctgctgttcg cagagattcg gttccgcagc ccatggcaca gactcaaaag tctgaagccc
     2401 atcacgatac gcaaatcgat ttcgatgaag tgcaagtgac ggctccttct cagcccaagg
     2461 cgcgacctga aaccccggta gcacgtccac gccccggcga gaatgacgcc actcgcgaaa
     2521 gcccctctac cgagatggcg cgcaaactga ccgcagagcc gatctcggct ggcttgtatc
     2581 acctgaccta cgcttgtttg ttggtgccgc gcttctcctc ccattacctg accggcgatc
     2641 tctcagaccg ccttggcgaa tggctgccta acatttgcat cgctttcggc tggcgcttgg
     2701 agttcttagc cgtgcgcccg gaatatttgc aatgggttgt gaacgtgcag ccgaatacat
     2761 cgcccggtca cttgatgcgc attatgcgcc agcagacttc agaaaaaatc tttggcgatt
     2821 tcccgcgcat gaagcgtgaa aacccctctg gtgatttctg ggcaccgggc tatctcatca
     2881 tgggcggttc acaaccacac ccgccacaac tggtgcgcga ttacattcgc caaacccgtc
     2941 agcgccaagg tctggaagac ctgccaccta tgaagtaggt ttctgaaaca aaagttctaa
     3001 ataaggaaac gcagagtata attaactctg cgttttttct tttagacact attccaattg
     3061 gtcactacac gactggatac tgacatgccc cctacccttt atctcattga tggacacgct
     3121 ctcgcctacc gcatgtactt cgccctcact gcaggcggag gcagccaacg ttggcagact
     3181 tcgaaaggtg aaccgacagc tggtatttac ggctttgcgc gtgaactggt gcgcgtgttg
     3241 gagcaggaaa aacctgaata ccttgccgtt gcctttgacg taggcaaaac cttccgcgac
     3301 cagatcttcc cggaatataa agcaacccgc gaaaagatgc ccgatgattt gcgttcgcag
     3361 attgtgcgca ttcgtgagat ggtgg
//
LOCUS       DAOREF010000255         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17959_length_3382_cov_3.851518, whole genome shotgun sequence.
ACCESSION   DAOREF010000255 DAOREF010000000
VERSION     DAOREF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17959_length_3382_cov_3.851518"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1178)
                     /locus_tag="PKI33_06495"
     CDS             complement(111..1178)
                     /locus_tag="PKI33_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HNM36690.1"
                     /translation="MNANQINATFDLVAIVPDLRKAGRYHVGPCPFCGGNDRFTIKQS
                     EKGSIWHCRNCGDGKYHTPIDFIMRREDCDFKTALKIMGGEVVTRDTYTQQRMPTVQA
                     APPSWSRPDAEWRSHTWQDAAWKTIDSCSERLLNNHEGELGREYLASRGLHVGTWGQW
                     FLGFEYHYDPREKRKRPAIVIPWIDFERIDNMVVMALKYRFIDQLAKQDKSKRFTQRA
                     GSVSVLYGLGYVMPAHHTLLLVEGEFNCISVNQCVPAGVTCLSFGSEGGGDMDVMQAI
                     ATRHSRVFVWADDLWDNPRQVKRAGELQVLSRTGGRLRSAVEGADKFDANELLKRGAL
                     PGFLSQILGVQCLGHLGAVLG"
     gene            complement(1358..2656)
                     /locus_tag="PKI33_06500"
     CDS             complement(1358..2656)
                     /locus_tag="PKI33_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM36691.1"
                     /translation="MMKTGAPTVDKFFVSRVEKPSKILTRRMMIDLIGRRVRGEVIDI
                     DPNSFSEPYKQCWLALDNAAPGKEFDTLKMIADESLLKQLAQQKAPTPYYPPMSEIQE
                     QIKDVTWYWDNRIPNGKLTVLGGESGVSKSMLAQRLCYLQLHEKQFPDGTPIHKPGEP
                     VLYVDCEGFASGIKKRAQAWQMDLSKFFLWSVDMENDGFINFSNPAFRDELIERVNSA
                     NPALVVIDSFGNASAGGQDKLEDVRDLLNFLNKVAYEFDLAILLIAHTRKPPAVFSGK
                     GEINQDDIRGSGHVVAMARSVIGVWLVQTGPEADPNGPRMMAVLKSNYARKPKPIGYD
                     VLNDANNNPMIFFGDAPKPYREPSKAQMCADWILETLTDEGAPMQPKELEVLAQKAGF
                     KRDTFYSAHRQLERGGLVKDTHGNKNPENAWALVDDREDG"
     gene            complement(2801..3184)
                     /locus_tag="PKI33_06505"
     CDS             complement(2801..3184)
                     /locus_tag="PKI33_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36692.1"
                     /translation="MRTRLLEGQTSARLKRKIKMQTHFTRSSTRDKSIYVKGRWIGKV
                     IADTFKKTISSSLILHSPYTAIANSVSVLNDAEALGAKYCEVFVSDTKQTYRATIKDI
                     RAKGETCGSRDDPQIKLALSEWRRV"
BASE COUNT          789 a          905 c          749 g          939 t
ORIGIN      
        1 ccgccggtct ccacccccaa gtcacagaca tttttgcccc cctcccccct tcgaagctcg
       61 aaaggacgta ggcggcatgt aaaaacgccc ctattcccta cgggcgaact tcaaccaagg
      121 accgcgccca gatgtccaag gcattgcacc ccaaggatct gcgagaggaa cccaggcaag
      181 gcgccacgct tcagcagctc atttgcatcg aacttgtcag caccctcaac agcgctgcgc
      241 aggcgtccgc cggtccttga tagcacctgc agctcccctg cccgcttcac ttgccgtggg
      301 ttgtcccata gatcatcagc ccacacaaag acacgtgaat gcctggttgc aattgcctgc
      361 atcacatcca tgtcaccgcc cccctcactt ccaaacgaga gacaggtcac gcctgcgggt
      421 acgcactggt ttacgctgat gcaattgaat tctccctcga cgagaagtaa ggtgtgatgt
      481 gctggcatga catagcctaa tccatacaac actgacacgc tgccggctct ttgggtgaag
      541 cgctttgatt tgtcctgctt tgccagttgg tctatgaaac ggtatttcaa agccatcact
      601 accatgttgt ctatgcgctc aaaatcaatc cacgggatga caatcgctgg tctctttcgt
      661 ttctcgcgtg ggtcataatg atattcaaag ccaaggaacc attgtcccca tgttccaaca
      721 tgaagaccac gagaggcaag atactctctt cccaattcgc cctcatgatt gttgagcagc
      781 ctttcactgc aggagtctat ggtcttccat gctgcatcct gccaggtgtg agagcgccat
      841 tctgcatctg gtcggctcca gctgggagga gctgcttgca ccgtcggcat gcgctgttga
      901 gtgtatgtgt ctctggtcac cacctcacca cccattatct tcaatgccgt tttgaaatca
      961 caatcctctc gccgcatgat gaagtcaatc ggcgtgtgat atttcccgtc accgcagttt
     1021 ctacagtgcc agatagaccc cttctcgctt tgtttgatgg tgaaccggtc attaccgcca
     1081 cagaacggac agggtccaac atgataccta ccagccttgc gcagatccgg cacgatggct
     1141 accaggtcaa aggttgcgtt tatttggtta gcgttcattt gtctactcca attcgttacg
     1201 ttgttacgat acatagtctc aaaaaatgag tgtgacttcc cgtaacagga gggaactgta
     1261 tgactgtatg acagttttaa caagaaattg ctttttcaca tcccccctat tgcaaagtct
     1321 taaaacagtc atacagtcag actcttgcgc gatttcccta cccgtcttca cggtcatcca
     1381 caagcgccca agcgttctcc gggttcttat tgccgtgagt atctttgacc aatccgcctc
     1441 tttcaagctg cctgtgagcc gaataaaagg tatctcgttt gaagccagcc ttttgagcta
     1501 agacttccaa ttcctttggc tgcataggtg caccttcatc ggtcaatgtc tcaaggatcc
     1561 agtcggcaca catttgtgcc tttgacggtt cccgatatgg ctttggagca tctccaaaga
     1621 agatcatggg attgttgttc gcatcgttca aaacatcata gccaatgggc ttgggctttc
     1681 tcgcatagtt ggatttgaga actgccatca tgcgaggacc attcggatct gcctccggac
     1741 ctgtttgaac caaccaaaca ccgatgacag agcgcgccat tgccacaaca tgaccactgc
     1801 ctcgtatgtc gtcctggttt atctcgcctt tacctgaaaa tactgcgggg ggtttacgtg
     1861 tgtgggcaat gagcagaata gcaagatcaa actcataggc aactttattg agaaagttca
     1921 ggaggtctct gacatcctct aacttatcct gtcctcctgc gctcgcattt ccaaatgagt
     1981 caatcacaac cagggcagga tttgcagagt tgactctctc aattaattca tcccggaagg
     2041 cagggttaga gaagttgata aaaccgtcat tctccatatc aaccgaccac agaaaaaact
     2101 ttgagagatc catttgccag gcttgagcac gcttcttaat gcctgaagca aacccttcgc
     2161 agtcaacata tagcaccggc tcccctggct tgtgtattgg agtgccatct ggaaattgtt
     2221 tctcatgaag ttgaaggtaa cagagccgtt gagcaagcat ggatttactg acaccgctct
     2281 caccgccgag gactgtcaat tttccattcg gaattcggtt atcccaatac catgtcacat
     2341 ccttaatttg ttcctggatt tcagacatcg gtgggtagta tggcgtcggc gccttttgct
     2401 gagcaagctg cttcaagaga ctctcgtcag caatcatctt caaagtatca aactctttcc
     2461 cgggtgccgc gttgtcaagt gcaagccagc attgcttata aggttcagag aaagaatttg
     2521 gatcaatgtc gattacctct ccgcgaactc gacgcccgat aaggtctatc atcatccgcc
     2581 gagtcaatat ttttgatggt ttttccactc gtgatacaaa gaatttatca accgttggcg
     2641 cacctgtttt catcatcttt aattctccag tgtcttattt aagatttttg ggtagaattg
     2701 gaaagagtgc cggtgacccg tcatcgtctc attcgcaact ctccaaattg cgctcgcctg
     2761 ctcaagcatg agcaggcgag acgcgttaaa ttgatcagcc ttagactctt ctccattctg
     2821 aaagggctag tttgatttgt ggatcatcgc gactcccgca ggtttcgccc tttgccctga
     2881 tatcctttat cgttgcgcga taggtctgct tggtatcact gacaaatact tcacaatatt
     2941 ttgcgcctaa ggcttcggca tcgttcagca ccgaaacact gtttgcaatc gctgtatatg
     3001 gtgagtgaag gatgaggctt gaagagatgg ttttcttgaa ggtatctgct ataacctttc
     3061 cgatccatct gccttttaca taaatggact tgtctcttgt tgagcttctg gtaaaatgag
     3121 tttgcatttt gattttcctt ttcagccgtg cgctcgtctg cccttccagc agacgagttc
     3181 gcatttaaac ggtctaatgg gttggttttc agacggcatc cgctggactc gcgcggttat
     3241 gtacatcccg caagtcttca gcatctaact tcaaataacg ctcaatttgt tttgggtctg
     3301 tgtgccctaa tagtttttga atttcaagta cttgtgttcc tcctctcctc attgccaggg
     3361 caaatgcacg acggaaagaa tg
//
LOCUS       DAOREF010000256         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_17986_length_3379_cov_4.638387, whole genome shotgun sequence.
ACCESSION   DAOREF010000256 DAOREF010000000
VERSION     DAOREF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17986_length_3379_cov_4.638387"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1417)
                     /locus_tag="PKI33_06510"
     CDS             complement(71..1417)
                     /locus_tag="PKI33_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HNM36693.1"
                     /translation="MSDKSVIIIGAGMAGLSAAHELNKAGWAVTVLEARSRVGGRVHT
                     VREFSHGLVAEGGAEYINESQHRMIALAKEFDLHLGAVGSWQNQSGDWAALQGKAGYL
                     HDTALWGADLDVENTKVWVALAELGKQVTDLSNPTASKNAKALDSQNAESWLQSLNVH
                     PLAKVLFANHIRSEYTCEPKDFSLLDLARNAAMYYSDPNASDPAYRIIGGNDQLPQAM
                     AKRLPDVRMNAVVTSIKLLDEKVEIVYKQADTFHTLQASYAILATPLTTARLIDFEGT
                     LPPAHHAMVHELSYGSITKVMIEYKKRFWHDRGWNGRLNTDLPIVFTWDATSHLEGEH
                     GILTAYTGGEPGAALTRLSDADRIKTAVSAIESIFPGSSNLIENTQTIAWRNEAFTLG
                     SYMAYAPNELTSHWQALFSPAGRLYFAGEHATIYQGFMEGAVESGQRAAKSIIDKG"
     gene            complement(1523..2047)
                     /gene="tpx"
                     /locus_tag="PKI33_06515"
     CDS             complement(1523..2047)
                     /gene="tpx"
                     /locus_tag="PKI33_06515"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNM36694.1"
                     /translation="MTTIRNDVLKLGDKFATLFGDDVKVGQAAPEFTSVVAGWAAVNP
                     IQESKGKVIILSAVPSLDTDVCDRETRRFNEEAAKLGDDIVIYTISTDFPMAQKRWCG
                     AAGVDKVKVVSDVLEAEFGIKYGLLIKERRFLRRAVFIVGHDGKFTYVNYLPTLGNEP
                     DYDEVISEAKKALG"
     gene            2167..2715
                     /gene="rfbC"
                     /locus_tag="PKI33_06520"
     CDS             2167..2715
                     /gene="rfbC"
                     /locus_tag="PKI33_06520"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNM36695.1"
                     /translation="MEFIPTRIPEIILVKPKIYEDPRGYFMEVYREDRFSIEGISRNF
                     VQENQSRSQKGVLRGLHYQIRQAQGKLVRVLSGEIFDVAVDIRRSAPTFGQWIGAILS
                     SENRHQLWIPEGFAHGFYVTSDSAEVAYKVTDYYAPEWERSILWNDPQIGVGWQLAKD
                     AAPILSQKDLNGKPLIDAELFE"
     gene            2741..>3379
                     /locus_tag="PKI33_06525"
     CDS             2741..>3379
                     /locus_tag="PKI33_06525"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNM36696.1"
                     /translation="MKNVLVTGGAGFIGSNFIHYLIQKESDVNIINLDALTYAGNLAN
                     LSSVEKHPRYRFVQGNICDADTIDSLLREYEIDTVVHFAAESHVDRSILGPRQFVETN
                     VMGTFTMLEAAQTYWIKDKAFPLEHVRFHHVSTDEVFGSLKPGEPAWTEETPYAPNSP
                     YAASKAASDHLVRSYGHTYGLPYTITNCSNNYGPYQFPEKLIPLMILNAIEGK"
BASE COUNT          880 a          787 c          772 g          940 t
ORIGIN      
        1 atacaccgat caaaataggc acttctgcac gtacgccttc aataaaattc cttcttgctt
       61 cactcattct ttatcctttg tctataatgc tttttgccgc acgttgacca ctctccacag
      121 cgccttccat aaacccttgg tagatggtgg catgttcgcc tgcgaaatat aatcttccgg
      181 caggggagaa gagcgcttgc cagtgtgaag tcaattcatt gggggcatat gccatgtaag
      241 accccaatgt gaaggcttcg tttctccagg caatggtttg ggtgttttcg atcaggtttg
      301 aagaaccagg aaaaatagat tcgatggctg agacggctgt tttgatgcgg tctgcatcag
      361 aaaggcgagt tagggcggca ccgggttctc cgcctgtgta agcggtgagg atgccatgtt
      421 cgccttcaag gtggctggtg gcatcccatg tgaagacaat cggcaggtct gtattgaggc
      481 gtccattcca gccgcggtca tgccaaaaac gctttttata ttcgatcatc actttggtaa
      541 tggacccata ggaaagttcg tggaccatgg catggtgagc aggaggcaaa gtcccttcga
      601 agtcgatcag gcgggcagtt gtaagaggcg ttgcaagaat ggcgtacgaa gcttgcaacg
      661 tatggaaagt gtcagcttgc ttgtaaacaa tctcaacttt ttcatccaac agcttgatgg
      721 aggttacaac agcattcatc cgcacatctg gcaatcgttt tgccatcgct tgcggaagtt
      781 ggtcattacc accaatgatg cgatatgctg ggtcagaggc atttgggtca ctgtaataca
      841 tggcggcatt acgtgccaaa tctaaaagag aaaaatcttt tggttcacat gtatattccg
      901 accgaatatg attcgcaaat agcacctttg ccaaaggatg aacgttcagg ctttgaagcc
      961 agctctctgc attttgacta tctaaagctt ttgcattttt agatgccgta gggtttgaaa
     1021 gatctgttac ttgttttcca agttctgcca gcgccaccca aacttttgta ttctctacat
     1081 caaggtctgc accccacaaa gccgtatcgt gtaaataccc ggctttgcct tgcaaggctg
     1141 cccaatctcc actttggttt tgccaggagc ctacagcccc caagtgaagg tcaaactctt
     1201 tcgcaagcgc gatcatgcga tgctgacttt cgttgatata ttctgcgccg ccttctgcta
     1261 caagcccatg cgaaaattca cgcacggtgt gaacacgtcc gcctacacgt gaacgtgctt
     1321 ccaatacggt gacagcccaa cctgctttgt tgagttcgtg cgcggcggaa agtcccgcca
     1381 tgcctgcgcc aatgatgatg acagatttat cgctcatagt ttgtggattt tatcattagg
     1441 atataaaaag ccgtcccaac atgcaggacg gcttcgcctc atagtcaaga cttgggctgc
     1501 gtactgccca tttagttttt atttagccga gagctttctt tgcttctgag attacttcat
     1561 cgtaatctgg ctcattcccg agcgtgggaa ggtagttgac atacgtgaat ttgccatcgt
     1621 gtcccacaat gaaaacggcg cgacgtaaaa agcggcgttc cttgatcaac agcccatact
     1681 tgatgccaaa ctcagcttca agtacatcag acacgacctt gaccttgtcc acaccggcgg
     1741 ctccgcacca gcgcttttgc gccatgggaa agtctgtgct gatggtgtag atcacaatat
     1801 cgtcacccag tttagcggct tcttcattaa agcgacgggt ttcacggtca cagacatctg
     1861 tatccagcga gggaacagcc gaaaggataa tcacctttcc tttcgattcc tgaattgggt
     1921 ttacagccgc ccaacctgct acaacactgg taaattctgg tgccgcttgc ccaactttca
     1981 catcatcccc aaaaagggtg gcgaatttat cgcccagctt aagaacatca tttctaatag
     2041 tagtcatttc ggttttctcc aaataggttt gttcaatttt atcagacgct acttttatgc
     2101 ccgcccttac tggaaatgga ttacagatat aatagagaaa aaaataagat ggagttttag
     2161 caaaatatgg aatttattcc tactcggatc cctgaaatta tcctagttaa gcccaaaata
     2221 tatgaagacc cacgtggata tttcatggaa gtttaccggg aggatcgatt ttctattgaa
     2281 ggaatctcca gaaattttgt tcaagaaaat caatctcgtt cgcaaaaagg tgttttgcgc
     2341 ggcttacatt accagatccg ccaggcacag gggaaattgg ttcgagttct ttcgggcgaa
     2401 atttttgatg tagctgtgga tattcgccgt agtgcgccaa ctttcggtca gtggattggg
     2461 gcgatccttt cttcagaaaa ccgccaccaa ttgtggatcc ctgaaggatt tgctcatggt
     2521 ttttatgtca ccagtgattc tgccgaagtt gcctataaag taactgatta ttacgcccct
     2581 gaatgggaac gtagtatttt atggaacgac ccacagatcg gtgttggctg gcaactagcg
     2641 aaagatgccg cacctattct ttctcaaaaa gatttgaatg gaaaacctct gatagatgct
     2701 gagttgttcg aataaagaga aataaggaca atactaaaga atgaaaaatg ttctggttac
     2761 aggcggagcc gggttcatag gctcgaattt catccattat ttgatccaga aagaatccga
     2821 tgtcaacatc atcaatttgg atgcgttgac ctatgctgga aatttggcaa acttaagttc
     2881 ggtcgagaag cacccgcgct accgttttgt gcaaggcaat atttgtgatg ccgacaccat
     2941 cgatagcctt ctgcgagaat atgagattga tacggttgta cactttgccg ctgagtctca
     3001 cgtagatcgt tccattttgg gacctcgtca attcgtagag acgaatgtga tgggcacctt
     3061 caccatgttg gaagccgcgc aaacttattg gattaaagat aaagcattcc cattagagca
     3121 tgttcgtttt catcatgtat ctacagatga agttttcggg tctctaaaac caggcgaacc
     3181 ggcttggacg gaagaaaccc cctatgcccc taattcgccg tatgcggcat cgaaagccgc
     3241 tagcgatcat cttgtccgct cttatgggca cacatatgga ttgccgtaca cgatcacaaa
     3301 ttgctccaat aattatggac cctatcaatt ccccgaaaag cttattcctt taatgatcct
     3361 aaatgcaatt gaaggtaaa
//
LOCUS       DAOREF010000257         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18053_length_3371_cov_3.785283, whole genome shotgun sequence.
ACCESSION   DAOREF010000257 DAOREF010000000
VERSION     DAOREF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18053_length_3371_cov_3.785283"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKI33_06530"
     CDS             complement(<1..199)
                     /locus_tag="PKI33_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM36697.1"
                     /translation="MFYFILSTILFLLALIIIYWIHNQYHLDIVVTPVTAPTSAPKVS
                     VCIPARNEETNIRRCVEAVLRQ"
     gene            complement(274..2856)
                     /locus_tag="PKI33_06535"
     CDS             complement(274..2856)
                     /locus_tag="PKI33_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNM36698.1"
                     /translation="MIPLHLRIAGFLSYRDPVDLDFNMVDLACISGHNGAGKSSLLDA
                     FTWSLFGEARGKGVDVINLSQDVKAAEVALTFLYEGNIYRVQRTLPRNKSTVLEFQVL
                     DRGSQTTDDGRWRPLTEKTTRDTQARIEQTLRLDYETFINASFFLQGKADQFTQKKAS
                     ERKAVLSAILGLEIWDTYKDRTAERRRELETEVTEIDGRIAEIDAELGEEDERRRRLS
                     ELEESLKQLTTAREAQESALENIRRNAALINEQRKTVANAETQLKRVQDTLTSIEAKI
                     AVKENERAEYLDLLERAKDIESAYKAWQKARKELEAFDANATEFREHDEKRQPLLREI
                     EVEKAKLEQEGEELGKQYSVISDYEAMVENLESQLNTAQESLKEAEEKIQERTKLEAQ
                     RNEARERQAGLKAENESLRREMDELKTRIDALKVAEGAECPLCGQPLSESHRKSTLKD
                     LEQTGKDKGDAFRSNKKEAEDLVEQIANYESRITKLSGADNERVRFSNSISQLTLQVE
                     SIRQQSKEWEKTGAKRLKEIEKVLESGKYASEAQKALKVLDKELTKLGYDVSAHEAKR
                     KEENDLRYVEEESRKLESARELSKRIGKEIQELEEERGKKKEETAEAEKVFSEAENVL
                     AESEAGAPNLQDAESKLFELREDENRVRDEAGAARQRVDVLATLRNRRAEYERQREEI
                     SQNIVRHKSLERAFGKDGVPALLIEQALPQIQQKANDILERLSDGTMMIQFVTQAEYR
                     DKKRDDLKETLDIHISDSSGMRDYEMYSGGEAFRVNFAIRLALSEILAQRKGARLQTL
                     VIDEGFGSQDALGRQRLVEAINAVKNDFSKIFIITHLDELKDAFPNRIEIEKTERGSS
                     VRVV"
     gene            2875..3342
                     /locus_tag="PKI33_06540"
     CDS             2875..3342
                     /locus_tag="PKI33_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36699.1"
                     /translation="MDYNRTTMLDAETPQARRRMRLLAIIIASIPCYCAGFVCLSFAP
                     NPRDITPTPSITETITGTPNTATASNTPLIVTSTITFTPTITSTPTTTSTPTATLTFF
                     LPSTNTFTFTPTTTLTFTPTNTFTPTASNTPTITATITNTPTPTTTLTPSPTP"
BASE COUNT          766 a          982 c          677 g          946 t
ORIGIN      
        1 cttgacgaag aaccgcctca acacaacgcc gaatgttggt ctcttcgttg cgagccggga
       61 tgcacaccga aacctttggc gcggacgtgg gcgcagtcac tggcgtgacg acgatatcca
      121 gatgatattg gttatgaatc cagtagatga tgatgagggc gaggaggaaa aggatggtgg
      181 agaggatgaa gtagaacatt ggtaaatagt aattggtaat tagagtttga cacttaccat
      241 ttaccaatta ctaatctcca attaccaatc tctttacacc acccgcaccg aactccctcg
      301 ctcggtcttt tcgatctcga tgcgattcgg gaaagcatct ttgagttcgt cgagatgagt
      361 aatgatgaaa attttagaaa agtcattttt gacagcgtta atcgcttcaa caaggcgttg
      421 acgtcccaat gcatcttgcg aaccaaagcc ttcatcgatg acgagcgttt gcaagcgagc
      481 acctttgcgc tgcgcaagaa tctcagagag tgccagccga atcgcaaagt tgacgcggaa
      541 ggcttcgccg cccgaataca tttcataatc acgcatgccc gaagaatcgc tgatgtgaat
      601 atcaagcgtt tctttcaagt cgtcgcgttt tttatcgcga tactctgcct gtgtgacgaa
      661 ttggatcatc atcgtaccat cgctgagacg ttcgagaata tcgttggctt tctgctgaat
      721 ttgcggaagc gcctgttcga tcaacaacgc tgggacgcca tctttgccaa aggcacgctc
      781 cagcgattta tgacgcacaa tattctggct gatttcctcg cgttgacgtt catactctgc
      841 ccgacgattg cgaagggtag ccagcacatc cacccgctga cgtgccgccc cggcttcatc
      901 gcgcacgcgg ttttcgtctt cgcgcaattc aaaaagcttg ctctcggcat cttgcaggtt
      961 cggagcaccc gcttcagatt cggctaaaac gttttcggct tcgctaaaca ccttctcagc
     1021 ctcagcagtt tcttctttct tcttgcctcg ttcttcttcc agttcttgaa tctctttgcc
     1081 aatacgcttg ctcaactctc gcgcagactc taacttgcga gattcttcct ctacatagcg
     1141 caggtcgttc tcttctttgc gttttgcttc gtgagcagaa acgtcatacc caagcttggt
     1201 aagctcttta tcaagcactt tgagcgcttt ctgtgcttca ctcgcgtact ttccgctttc
     1261 taataccttt tcaatttctt tcaagcgctt cgcgccggtc ttttcccatt ctttgctctg
     1321 ctgtcgaatc gactctacct gcaaagtcag ctgtgaaatg gaattagaga agcgcacacg
     1381 ttcgttatca gcgcctgata atttcgtaat tcgcgattcg taatttgcga tctgttcaac
     1441 gaggtcttct gcttcttttt tgttagaacg gaacgcatcg cctttgtctt tgccggtctg
     1501 ctccaaatct ttcagagtag atttacgatg gctttcgctc aaaggctgcc cacacagcgg
     1561 acattccgca ccttccgcaa ctttcaaggc atcgatgcgg gtcttgagtt catccatctc
     1621 ccgccgcaga gattcattct ccgctttcaa gcctgcctgt cgctcccgcg cttcgttgcg
     1681 ctgagcttcc aatttagtac gttcctgaat tttttcttct gcttctttta gcgactcttg
     1741 cgcagtattc aattgacttt ccaggttttc aaccatcgcc tcatagtcac tgattactga
     1801 atactgttta ccgagttcct ccccttcttg ctccaacttc gccttctcta cttcaatttc
     1861 gcgcaacaag ggttgacgtt tctcatcatg ttcgcggaac tctgttgcgt tggcatcgaa
     1921 cgcctccaac tctttacgcg ctttctgcca cgccttatac gcagactcaa tatcctttgc
     1981 ccgttccaac aagtccaaat attcggcgcg ttcattttcc ttgacagcga tcttcgcctc
     2041 aatggaagta agcgtatctt gtacccgttt caactgcgtt tcggcgttcg caactgtttt
     2101 tctctgctca ttgatcaaag ctgcatttcg gcggatgttt tccaatgcag attcttgtgc
     2161 ttcgcgcgcc gtcgttaact gcttcagtga ttcttctaat tcgcttaaac ggcggcggcg
     2221 ttcgtcttct tcccctaatt ccgcgtcgat ctccgcaatg cgaccatcaa tttcagtgac
     2281 ttcggtttcc aattcacgac gacgttctgc cgttcggtct ttgtaggtgt cccaaatttc
     2341 caacccgaga atggcgctta gcactgcctt tcgctcgctg gctttcttct gcgtgaattg
     2401 gtcggctttg ccttgcaaaa agaacgaagc attgataaac gtctcataat cgaggcgtaa
     2461 ggtttgttca atgcgggctt gcgtgtcacg ggtcgttttc tccgtcagcg gacgccaccg
     2521 tccatcgtcg gtcgtttgtg acccgcggtc taagacctga aactccaaaa ccgtactctt
     2581 attgcgtggc aaagtccgct gcacgcgata aatattgcct tcatataaaa acgtgagggc
     2641 aacttcagca gcttttacgt cctggcttag gttgataaca tccacccctt taccgcgtgc
     2701 ctccccaaac aatgaccatg taaacgcatc caataaagaa gacttgcccg cgccgttatg
     2761 cccagaaata caagcaaggt ctaccatgtt gaaatccaag tcaacagggt cacgataaga
     2821 gagaaaacca gcaatacgaa ggtgaagtgg aatcattaaa tgctacccaa tcgcatggat
     2881 tataatcgaa ccactatgct agacgccgaa acacctcaag cacgccgccg catgcggctt
     2941 ctcgcaatta ttattgcatc cattccttgt tattgcgccg ggtttgtttg tctttccttt
     3001 gcacccaacc cgcgcgatat cacccctacc ccatccatca cagagacgat cacaggcaca
     3061 ccgaataccg ctactgccag caacacccca ttgatcgtca ctagcaccat tacgtttaca
     3121 ccaactatta cctccacccc caccaccaca tccacgccta cggctacgct cacgttcttc
     3181 ctgcctagca ccaacacatt tacatttacc cccaccacaa ccctcacctt cacgcccacc
     3241 aatacattta cgccaactgc atcgaacacg ccaaccatta cagcaacgat caccaataca
     3301 ccaaccccaa caaccacact cactccatct cccaccccat aacttcatga gcgatattct
     3361 ccccttccta a
//
LOCUS       DAOREF010000258         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18081_length_3368_cov_4.635678, whole genome shotgun sequence.
ACCESSION   DAOREF010000258 DAOREF010000000
VERSION     DAOREF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18081_length_3368_cov_4.635678"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKI33_06545"
     CDS             <1..532
                     /locus_tag="PKI33_06545"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNM36700.1"
                     /translation="VDGKDQMTMVSRFTWTNWAKGRVLKQTENLWQGEHDGYNLVRHK
                     RTVMALEGDRWLVIDDLEANEPHHYTLHWLLNDVPFTQNKNSLLLKYEASTYKIQTGL
                     TNGEGKFSILRADAHSTYGWRSRYYGHKEPAISILLEADQPTSRFYTFFGFENDILTV
                     TENQLNLNSKIYSFGE"
     gene            586..1392
                     /locus_tag="PKI33_06550"
     CDS             586..1392
                     /locus_tag="PKI33_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM36701.1"
                     /translation="MKRLSAFQLVIPCALIVQACGNSVPSAEPTPKIELAITPVESAF
                     APENPTAAPTEEIMQNHAVTFTTPDGATLNGELYGSGDTAVIFSVMGNCDPGWREFAQ
                     LTAAQGMMSLTYLWRGCRAAGSANEAEMKEFVDDLRGAIAFMRAQGAKKIILVGASLG
                     GCASAKLTVESQVDGLVVVASPAEIPQWGFAIEASDVDADIPKLFITAEEDDTVPFEA
                     SQGLFDLAAEPKEWQTYPGTAHGTELFETQSKDEFQQRILEFILAVKAVN"
     gene            complement(1389..1727)
                     /locus_tag="PKI33_06555"
     CDS             complement(1389..1727)
                     /locus_tag="PKI33_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36702.1"
                     /translation="MNDIIPCLGVVGSLTIIFGFLAFLRYMNYKETIVLAEKGLTKSE
                     SKPNKRLLSWGIVITALGFALSLGLYTIGFDTGNNYPLHLGPWMLGGFVPLFLGLGLV
                     LLHYLTEKDS"
     gene            complement(1806..3113)
                     /locus_tag="PKI33_06560"
     CDS             complement(1806..3113)
                     /locus_tag="PKI33_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNM36703.1"
                     /translation="MKRPIVITLLIVALVLVCAGIGSVIYFANGFSVNNPFDKLNISS
                     VVEESKTLKVDTTKPLTLKVLDDAGAVTITGDDVDTVQVKIVKTAYDSSQARADAEVK
                     DIKYTIDQTGGNITLKYELPKSVNFSNHINTVDFIVTVPNEVIVDINSSSGDVNVANT
                     KGNINIKNSFGDVTVENIEGALTVHTNSGKVTATSIVANTENIDLSSDFGDVILKKAG
                     GKDISLDSNSGNILLSEVRATGDLAANTDFGNTEFENGSADALKVETNSGKVSLTKLK
                     ISKLLFVKNDFGDIELDQAMAGSYDLHTNSGDITVDGAKGKLKADTDFGNVTISNAQS
                     VTLTLETNSGTIEFSGSLGVGPHFVKSDFGGIDISLPVDAKLTVDLKTDFGKINSELP
                     VTVVLTDGAKSDGDQIAGDINGGGEQLTVQANSGNISIRVSSK"
BASE COUNT          867 a          802 c          797 g          902 t
ORIGIN      
        1 agtagacggt aaagaccaaa tgaccatggt cagccgcttc acctggacaa actgggcaaa
       61 gggacgagtc ctcaagcaaa ccgagaatct ctggcaaggt gaacacgacg gctataacct
      121 tgtccgccac aaacgtacgg tcatggcact ggaaggggat cggtggttgg tcatcgacga
      181 ccttgaagcg aatgagccgc atcactacac cctgcactgg ctcctgaatg atgtgccctt
      241 cactcaaaac aaaaactcgc ttcttctgaa atacgaagcg agtacctaca aaattcaaac
      301 cggactaacg aacggcgaag gcaaattctc catcctccgc gcggatgctc actccactta
      361 tggctggcgc tcgcgctatt acggtcacaa agagccagcc atttcaatct tgcttgaagc
      421 agatcaaccg acttctcgct tctacacctt cttcggcttc gaaaacgata ttctcactgt
      481 aaccgaaaat cagctgaacc tcaattcaaa gatatattcg tttggcgaat aaaccagtcc
      541 gctagagggg tgacattcaa atcggatttc attatattga ttcctatgaa aagattatca
      601 gcctttcaat tggtcattcc ttgtgcactt atcgttcaag cttgcggcaa ttctgtccca
      661 tctgcagagc caacacccaa aatagaactt gccatcaccc cggttgaatc tgcttttgca
      721 cctgagaacc ccaccgcagc accaacggag gaaattatgc aaaaccatgc tgtcacattt
      781 accaccccag atggtgcaac tctaaacggt gagctttatg gcagtggaga taccgccgtc
      841 attttctctg tcatgggcaa ttgtgaccca ggttggcgcg agtttgccca actcaccgct
      901 gctcaaggta tgatgtccct tacatatctc tggcgcggat gccgagcagc tgggtctgcg
      961 aatgaagctg agatgaaaga atttgtggac gacctgcgcg gcgcaatcgc cttcatgcgt
     1021 gcgcaaggag caaagaaaat tattttggta ggtgccagcc tgggcggatg tgcctctgcc
     1081 aagttgacgg tcgaatcgca agtggatggg ttggtggttg tggcatcccc agccgaaata
     1141 cctcagtggg gctttgccat cgaagcaagt gatgtcgacg cagatatccc caaattattt
     1201 attaccgccg aagaagatga cactgtgcca tttgaagcgt cacagggatt gtttgatcta
     1261 gcagcagaac ccaaagaatg gcaaacctat cccggcacag cgcatggcac agagttgttt
     1321 gagacgcaaa gtaaagatga atttcagcag cggattctgg aatttattct cgcggtcaag
     1381 gctgtgaatt aggaatcttt ctcggtcagg tagtggagca ggaccagccc caagccaaga
     1441 aaaagcggaa cgaagccgcc caacatccag ggtccgaggt gaagcgggta gttattcccg
     1501 gtatcaaaac caatggtgta gagtccgagt gagagtgcga atccaagtgc ggtgatgaca
     1561 atcccccaac tcaaaagtct tttgttcggt tttgattctg atttggtcaa tcccttttcg
     1621 gcaagcacaa tggtttcttt ataattcatg tagcggagaa aggcgagaaa gccaaagatg
     1681 atggtgagtg agccaaccac gccaagacag ggaatgatat cgttcatagt gagtcctttg
     1741 ctggcaatga tagcatataa aaaatccggt gacagttact tactgtcacc ggattttttt
     1801 atgctttatt tgctgctgac cctgatgctg atgttgccgc tgttggcttg tacggtgagt
     1861 tgttcaccgc cgccgttgat atctccagcg atctgatcgc cgtctgactt ggcgccgtca
     1921 gtcaagacca ctgtgacggg taactccgag ttgatcttcc caaaatcagt tttgagatcc
     1981 acagtcagtt ttgcatcgac aggtaggctg atatcaatgc caccaaaatc ggatttgaca
     2041 aagtgtggac ccacacccaa tgagccgcta aattctatgg tgccgctgtt cgtctcaagg
     2101 gtcaatgtaa cggactgagc attgctgatc gtgacattgc caaaatctgt atcggctttg
     2161 agcttgcctt tggcgccatc tactgtgatg tcaccgctgt tggtgtgcaa gtcataagaa
     2221 cctgccatcg cttgatccag ttcgatatcg ccaaagtcat tttttacaaa aagaagcttg
     2281 ctaattttta atttggtgag agataccttg ccgctattgg tttcgacctt cagcgcatct
     2341 gccgagccat tctcaaactc tgtgttgccg aaatctgtgt ttgctgcgag atcacctgtg
     2401 gcgcgcactt cactcaaaag gatgttgccg ctgtttgagt cgagggaaat atctttgccg
     2461 ccagcttttt tcaagatgac atcaccaaag tctgaactca gatcaatgtt ttcggtattt
     2521 gccacaatgg acgtggcagt caccttgccg ctattggtgt gaacggttaa tgcgccttca
     2581 atattttcta cggtcacatc accaaagcta ttcttgatat tgatattgcc tttggtgttg
     2641 gcaacattga catcacctga gctgctgttt atgtcgacta tgacttcgtt aggaactgtg
     2701 acaataaaat ccacggtgtt gatatggttg ctgaagttta cagatttagg cagttcgtat
     2761 ttgagcgtga tgttgccgcc ggtttggtcg attgtatatt tgatatcttt aacttctgca
     2821 tctgcgcggg cttgtgatga atcgtaagcg gtcttgacta tcttgacttg tacagtgtct
     2881 acatcgtcgc ctgtgatggt gacagcgcca gcatcatcga gtactttcag tgtgagaggt
     2941 ttggttgtat ccactttgag ggtcttgctt tcctctacca cggatgaaat attcaactta
     3001 tcaaacgggt tgttgaccga gaagccattg gcaaaataga tcactgagcc gattcccgcg
     3061 cataccagca cgagtgctac aatcaatagg gtaataacaa ttggtctttt cattctattt
     3121 ctccttgaat tttgtttctt attacgtttg acaggtattt ttggaaaaag tttcaagatc
     3181 gggtgataga ccacaaagtg aaacttttcc gccctttccc tgtctaaccc agtagaaaat
     3241 gacagtctct gagcagatcc tcgttcaaaa agcccaaaaa ggcgacgctc aagcctttga
     3301 aacgctggtg aacgagcacc aacaatacgt ctacaacctt gccttgcgcg tcgtcaaaga
     3361 tgagaacg
//
LOCUS       DAOREF010000259         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18115_length_3365_cov_4.433535, whole genome shotgun sequence.
ACCESSION   DAOREF010000259 DAOREF010000000
VERSION     DAOREF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18115_length_3365_cov_4.433535"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /gene="coaBC"
                     /locus_tag="PKI33_06565"
     CDS             <1..904
                     /gene="coaBC"
                     /locus_tag="PKI33_06565"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNM36704.1"
                     /translation="GIADNLLTVTALAATCPIMIAPAMDGGMFDHPATQANLQTLQSR
                     SVLVAGPAAGHLASGMSGVGRMLEPADILGHIRMALGKSGKLAGKKVIVTAGGTQEAI
                     DPVRVITNHSSGKQGYALAQAALDAGAKVTLITTPTAITPPVGADVIHVKSVQDMLDA
                     LLQESADTLIMAAAGADFRPVNVAKDKLKKRDGIPQIQLEAAPDILKTLSSQGVEKKR
                     FQVTVGFAAESRDLLANASEKLKSKGLDFIAANDISANDAGFAVETNRITLLFANGTQ
                     ETLPLMSKSEVAEKIIEHIARLLE"
     gene            906..1637
                     /locus_tag="PKI33_06570"
     CDS             906..1637
                     /locus_tag="PKI33_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNM36705.1"
                     /translation="MRILVMSDIHANYTALEAVLKDAGSVDETWCLGDLVGYGPDPNA
                     VVEEVQEIKNLTCLMGNHDVAVVGRMSLETFNGDAKRSLMYHEKVLTASNIDFIKSLP
                     SKTKVRGEASMAHGSPRDPLWEYILNSMTASLNFEHFDTPWCFVGHTHIQSSFSQVDA
                     SGRVLVEQTRPDVTLQLQPKLILNPGSVGQPRDRDPRAAYAIYDTEARTWTPKRVMYN
                     IAEVQERIRAAGLPEKHAVRIGEGW"
     gene            1775..2755
                     /locus_tag="PKI33_06575"
     CDS             1775..2755
                     /locus_tag="PKI33_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNM36706.1"
                     /translation="MKRLTPYSLILIAMIVLGACASAPRAVELSGGSAPASEPYYDQS
                     SVMPAPTMMYATDGIIAEEAQVSGEGSGNANAAQVERIVIQNADIAVVVSDVELRMDE
                     IQKMAVDLGGFVVSSNLYQSYTSDYTPVPEASLTIRVPAENLDEALEKIKKDAVEVQN
                     ETRSGQDVTAEYVDLKSRLKTYQAAEEELTQLMDNAQNSDEVVNIFNQLMYYREQIEI
                     IQGQIKYYEEAAALSAISIRLIAEETTKPVTIGKWEPKGVALEAIQDLLDFWKGFVDF
                     MIRFVIFTLPMLITIGIPFYLAFIGVRWVFRKMRGNKKKVEPQVKEEEKK"
     gene            2815..3357
                     /gene="rsmG"
                     /locus_tag="PKI33_06580"
     CDS             2815..3357
                     /gene="rsmG"
                     /locus_tag="PKI33_06580"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNM36707.1"
                     /translation="MDSLIQDAKTLFNVHLTARQVVALVNYEKELMEWNQKFNLTAIR
                     DVESIRTKHFLDSYSCVLAWKANPPQRLIDVGTGAGFPGIPLKIIYPTMQVTLVESVG
                     KKAMFCQHVIQTLGLEGIEVLHSRAEDVGQNPVHREAYDCTARCSRGPRRRDSERTRR
                     RSGTSPSRCSWRTRRTTWVS"
BASE COUNT          873 a          848 c          884 g          760 t
ORIGIN      
        1 tggcattgcc gataatttac tcaccgtcac agccctcgcg gcgacttgcc cgataatgat
       61 cgcgcccgcc atggatggtg ggatgttcga ccaccccgcc acgcaagcca atctccaaac
      121 gcttcagtct cgctcggtgc tggttgccgg acccgccgcc gggcacctcg cctcaggcat
      181 gagcggcgta ggacgcatgc tcgaaccagc ggatattctc gggcatattc gcatggcgct
      241 tgggaaaagc ggaaaactcg caggcaagaa agttatcgtc accgctggtg ggacgcaaga
      301 agccatcgac cccgtacgcg tcatcaccaa tcattcctct ggcaagcaag gctatgccct
      361 tgcccaagct gcgctcgatg caggtgcaaa agtaaccctt atcactacac ccacggcgat
      421 taccccacct gtaggcgcag atgttatcca tgtgaaaagc gtgcaagata tgctcgatgc
      481 tcttctgcaa gaatctgccg acacgcttat catggctgct gctggagcag acttccgccc
      541 ggtgaatgtg gcgaaggata agctcaaaaa acgcgatggc attccgcaga ttcagcttga
      601 ggcggcacca gacatcctga aaacgctgtc aagccaaggc gtagaaaaga agcgatttca
      661 agtcacagta ggattcgcag cagagagtcg cgacttgctg gcgaatgcat ccgaaaagct
      721 gaaatccaaa ggcttggact ttatcgccgc gaatgacatc tctgcaaacg atgccggttt
      781 tgcagtggag accaatcgaa ttacgttgtt gtttgcaaat ggaacacagg aaacattgcc
      841 actcatgagc aaaagtgaag tggctgaaaa aatcatcgaa cacattgcga ggctgttgga
      901 ataatatgcg tattttggtc atgtcggata ttcatgcgaa ctatacggcg ttggaagctg
      961 tattaaaaga tgcggggtct gtggatgaaa cgtggtgcct tggtgatctt gttggatatg
     1021 gaccagaccc aaacgcggtg gtggaggaag tgcaggagat caagaacctg acttgtctga
     1081 tgggcaatca tgatgtggcg gttgttgggc gcatgtcgtt agagacattc aacggcgatg
     1141 ccaagcgttc gttgatgtat cacgagaaag tgcttaccgc cagcaatatt gatttcatta
     1201 agtctctgcc gagcaaaacc aaggtgcgcg gagaagcgtc tatggcgcat ggcagtccgc
     1261 gcgacccgtt gtgggaatac atcctcaact cgatgaccgc gagtttgaac ttcgaacatt
     1321 tcgatacccc ttggtgcttt gtggggcata cccacatcca aagtagtttt tctcaagtag
     1381 atgcttcagg acgtgtgcta gttgagcaga ccagacccga tgtgacgctt caacttcagc
     1441 cgaaactgat tctgaatccc ggcagtgttg gtcaaccccg agaccgtgac ccgcgtgccg
     1501 cttacgccat ttatgacacc gaagctcgca cctggacgcc aaaacgtgtg atgtataaca
     1561 tcgccgaagt gcaagagcgc atccgcgcgg cgggattgcc agaaaaacat gccgtgagaa
     1621 ttggcgaagg ttggtaacgc ctcccacaaa agtcctccct gaagtttggg agggcttttt
     1681 ctttatagga agttgtacaa tacgtgggaa caatttattt tagcgtagcg tcctttgaag
     1741 agagaaatta ccaaaggaaa agaaggagaa agaaatgaaa cgcctaacgc cctatagtct
     1801 tattttgatt gcaatgatcg ttcttggagc ttgtgccagt gctccgcgcg ccgtggaact
     1861 ttctggcggg tctgccccag cttcggagcc ctattatgac caatcctctg taatgcccgc
     1921 accgaccatg atgtatgcta ctgatggcat tatcgctgaa gaagcccaag taagtggaga
     1981 aggcagcggg aacgccaatg ccgcccaagt ggaacgtatt gtcattcaga atgcagatat
     2041 tgcagttgtg gtctcagatg tggaactgcg tatggatgag atccaaaaaa tggcagtcga
     2101 tcttggcgga ttcgtagtgt cttccaattt gtatcagagc tataccagtg attacacccc
     2161 ggtgcctgaa gccagcctga ccatccgcgt gcctgctgaa aatctggatg aagcgctgga
     2221 aaagatcaaa aaagatgcgg tggaagttca gaacgaaacc cgctctgggc aagatgtcac
     2281 cgctgaatac gtggacttaa agtcccgcct caagacctat caagccgctg aagaagaact
     2341 gacccaattg atggacaatg cacaaaactc agatgaggtc gtcaatatct tcaatcagtt
     2401 aatgtactac cgcgaacaga tcgagatcat tcagggtcag atcaagtatt acgaagaagc
     2461 tgccgctctc tctgccatta gcattcgtct catcgccgaa gagaccacca aaccagtaac
     2521 gattggcaaa tgggaaccga agggtgttgc gctcgaagcc atacaagacc tgctcgattt
     2581 ctggaaaggc ttcgtcgatt tcatgatccg cttcgtgatc ttcaccttgc ccatgttgat
     2641 caccatcggt attccgttct acctggcatt catcggcgtc cgctgggtct tccgtaagat
     2701 gcgtggcaac aagaaaaaag ttgaaccgca ggtgaaggaa gaagaaaaga agtaaaacgt
     2761 cataacacga gaggacggtg taccgccgtc ctctttgttt tataatcttc gcatatggac
     2821 tctctgattc aagacgcaaa aacattattc aacgtccacc tcacggcgcg acaggtggtg
     2881 gcgctcgtca attacgaaaa agaattgatg gagtggaacc aaaaattcaa ccttactgcc
     2941 attcgggatg tagagtctat tcgtaccaag cattttctgg attcatattc ctgtgtgctg
     3001 gcgtggaaag ccaacccgcc gcaacgcctc atcgacgtgg gcacgggcgc tggtttcccc
     3061 ggcattccgc ttaagattat ttaccctacc atgcaagtca ccttggttga atcagtcggc
     3121 aagaaagcca tgttctgtca gcatgtgatt caaacgctgg gcttggaagg catcgaagta
     3181 ttgcattctc gtgccgaaga tgtaggtcaa aacccggttc atcgtgaagc ctatgactgc
     3241 acggcgagat gttcccgcgg cccgcgccgg agagattccg aacgcacgcg ccgacgatcg
     3301 ggcacatcgc cgtcacgctg ctcgtggcgc acccggcgca ccacctgggt cagttgaggc
     3361 agtgg
//
LOCUS       DAOREF010000260         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18172_length_3359_cov_4.815981, whole genome shotgun sequence.
ACCESSION   DAOREF010000260 DAOREF010000000
VERSION     DAOREF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18172_length_3359_cov_4.815981"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PKI33_06585"
     CDS             complement(<1..929)
                     /locus_tag="PKI33_06585"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNM36708.1"
                     /translation="MPNTTFNSRRSPVYSRRGIVATSQPLATAAGIEVLSKGGNAADA
                     AVAAAAALNVTEPTSTGIGGDMFALYFDAQTQQVTALNGSGRASSVLTLERLKREGLL
                     ADSLPLFHPHTVTVPGACAGWCDLIAKHGSLSLTEILAPAIRLADEGFPVAPITAHFW
                     ARGADRQLKSALNGHELTIDGRAPKAGEIFQNKGLARTFKLVAERGALGFYQGSVAES
                     IVAVIKEAGGCLSAEDLASHVSTWETPLSVDYRGLRVYECPPNGQGITALIALNILEG
                     FDLAELDLLSTEKMHLMIEAMRLAFADAKWYVS"
     gene            1046..2461
                     /locus_tag="PKI33_06590"
     CDS             1046..2461
                     /locus_tag="PKI33_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36709.1"
                     /translation="MKKLFSLFFAISLILSACGTVSTTPAQTGMPIVTHTATQTVTST
                     ITPLPTFTPTFDVSTIVTVTPAEKAECPTSIPYPEKDFDLFEFWENGNYQSPEEAALA
                     LLNKYGSEVLVNYERSFGDSPETMAFQDFTNDGVPELAIRLTLNTSFSSFDIFGCQDG
                     EYHLLLSIPSNGLGYAPIIVSIEDNNKNSVPELTILEGYLSQGGHSFGVYEWDGEKFT
                     SLLPPNHPDQPDSTFLWVEATGKIYYKDVDSDFLNELILESGIPVWEIYYVGLPWRNN
                     STYYKWNGQVFAPYKSEFTEPEFRFQAVQDADLAFTQQEYGKAVVLYQEAIFSDELRS
                     YSPEIRENLRAIWEAQYGEVPTPTPYPIEDPTEYPSLAAYAYYRIMLLHLVQGQEAEA
                     ESTYQTLQDTFGADPYAASYVEMATKFWEAYQSTNKMYDGCAAAIQYAVEHPQILTPL
                     GSDYHGWQSHIYVPADVCPFR"
     gene            2547..>3359
                     /locus_tag="PKI33_06595"
     CDS             2547..>3359
                     /locus_tag="PKI33_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNM36710.1"
                     /translation="MTQVYDYVIIGSGFGGSVSAMRLTEKGYSVLVLEKGKRFEDKDF
                     AKSNWQYWKYVWIPALRAHGILQISILKGVMVLHGAGVGGGSLGYANVLEVPTDETFA
                     TPAWSQNIKWGDVLRPHYETAKRMLGVARNPKLWKADLLLKEMAEERGMGHTFRATDV
                     GSYFGEAGVTVPDPYFGGEGPARAGCQHCGGCMVGCRHNAKNTLPKNYLYFAEKRGAK
                     VQAEVEVIDVKPLNPNYEITYRDSTKLFKQSHTVHAKNVIFSAGVMGTMKLLL"
BASE COUNT          887 a          854 c          823 g          795 t
ORIGIN      
        1 tcactcacat accatttcgc atcagcaaac gcgaggcgca tggcttcgat catcaggtgc
       61 attttttcgg tcgagagcaa atccaattcc gcaaggtcaa agccttcgag gatgttcaac
      121 gcgatcagag ccgtgatgcc ctgtccattt ggcggacatt catacacgcg cagtccacga
      181 taatctaccg aaagcggagt ctcccaagtg gacacatgtg acgcgaggtc ttcagcggat
      241 aggcacccgc ccgcctcttt tattacggcg acaattgatt ctgcaaccga gccttggtaa
      301 aagccgagag cgccccgctc cgcgacgagt ttgaacgtgc gtgccagacc tttgttttga
      361 aagatttcac ccgccttcgg cgcacgcccg tcgatggtta actcatgccc attcaacgcg
      421 gacttcaatt gcctgtctgc accgcgtgcc caaaaatgag ccgtgatcgg tgcgacggga
      481 aaaccttcat ccgcaagtcg aatcgcaggc gcgaggattt ccgtcagcga gagcgagccg
      541 tgctttgcga tcaagtcaca ccagcccgca cacgccccag ggacagtcac cgtatgcgga
      601 tggaacagcg gcaaactgtc agcgaggagt ccctcacgct ttagccgctc aagcgtcagc
      661 acggacgagg cgcgccccga tccgttcagg gcagtcactt gctgagtctg cgcgtcaaaa
      721 tacaaggcga acatatctcc gccaatcccc gtggaggttg gctcggtaac attgagcgcg
      781 gcagcggcag ccacagcggc atcagcggca ttgcctccct ttgaaaggac ttcaatgccc
      841 gcggcagtag ccaacggctg cgaggtggcg acaattccac ggcgggagta cacgggcgaa
      901 cgacgagagt tgaatgtggt gttgggcatg ggtttctcta tgaattggtt ttgctgaaag
      961 ctgagcgctg attgctagat agcgttcagc tttcagcact cagccagtat tttctcgaag
     1021 tataatccca tcaatgaggt ggacgatgaa aaaacttttc tcccttttct ttgcaatcag
     1081 cctaatcctc tccgcttgtg ggacagtatc caccactcct gcacaaacag gaatgccaat
     1141 cgtaacgcac acggcaactc aaacagttac ttccaccatt acacccctgc ccaccttcac
     1201 cccgactttc gatgtttcga cgattgtcac ggttacgcct gcggagaagg cggagtgccc
     1261 aacatccatc ccatatcccg aaaaagattt tgatcttttt gaattctggg agaacggaaa
     1321 ttatcaaagt cctgaagaag ctgccttagc attgcttaac aaatatggat cagaagtact
     1381 ggtgaactac gaaagaagtt ttggggatag tccggaaacc atggcttttc aagatttcac
     1441 taatgacgga gtacctgagt tagctattag attgaccctt aatacttcat ttagctcatt
     1501 tgacatattc ggttgtcaag acggtgaata ccatctttta ttatccattc cttcgaatgg
     1561 attaggttat gctccaatta ttgtgtcgat tgaggataac aataaaaaca gcgtgcccga
     1621 gttgacgata ctagaaggtt acttatctca aggaggacat tcatttgggg tctacgaatg
     1681 ggacggtgaa aaatttacaa gccttcttcc tccaaatcat ccagatcaac cagattcaac
     1741 ttttttatgg gtagaggcaa ctggaaaaat ctattacaaa gatgttgatt ctgattttct
     1801 aaatgagtta attttggaaa gtggcattcc ggtctgggaa atttattacg ttggtctgcc
     1861 atggcgtaat aacagtacat attacaagtg gaatggtcaa gtctttgccc catacaagag
     1921 cgaattcaca gaacctgagt ttagatttca agctgtacaa gatgctgacc tcgcatttac
     1981 tcagcaagag tatggcaaag cagtagtctt ataccaagaa gcgatattta gtgacgaatt
     2041 aagaagttat tctccagaga tacgtgaaaa ccttcgagca atctgggaag cacagtatgg
     2101 agaagtccct accccaacgc catatccgat tgaagaccca accgaatacc caagcctcgc
     2161 cgcttacgcc tattaccgca taatgctcct tcacctcgtg caaggacaag aagcagaagc
     2221 cgagtccacg taccaaactc tgcaagatac cttcggcgca gacccatacg ccgcttccta
     2281 cgtggaaatg gcgactaaat tttgggaagc atatcagtcc acgaataaga tgtacgatgg
     2341 ttgtgcggcg gcgatccaat acgcggtgga gcacccgcag attctgactc cgctcggcag
     2401 tgattatcac ggctggcaaa gtcacattta tgtgcctgcg gatgtctgtc cattcaggta
     2461 ataacttagg tgacagtcag attaaagctg actgtcacct tttgtaactt gatataatca
     2521 cgctaattca atgcggaggg cacggaatga ctcaagtcta tgactacgtg attattggaa
     2581 gtggcttcgg gggaagtgta tccgccatgc gattgaccga aaaaggctac tcggttctcg
     2641 tccttgaaaa aggaaaacga tttgaagata aggattttgc caaatccaat tggcaatact
     2701 ggaagtatgt ttggatcccc gctctgcgtg cacatggcat tttgcaaatt tccatcctca
     2761 aaggcgtgat ggttctgcac ggcgcgggcg tgggcggtgg cagccttggc tacgccaacg
     2821 tgctcgaagt tcccaccgac gaaacctttg caactcccgc ctggagtcaa aatatcaaat
     2881 ggggcgatgt gcttcgtcca cattatgaaa cagcgaaacg catgctcggc gttgcccgca
     2941 accccaaact ttggaaagcg gatttgctgc tcaaagaaat ggcagaggaa cgcggcatgg
     3001 gtcacacctt ccgcgcgacg gatgtcggct cctacttcgg ggaagctggg gtgaccgtcc
     3061 cagaccccta cttcggcgga gagggtcccg cacgcgcggg ctgtcagcat tgcggcggct
     3121 gcatggtggg atgccgtcat aatgcaaaga acactttacc gaaaaattat ttatactttg
     3181 cagagaagcg cggcgcgaaa gtgcaggcag aagttgaagt cattgatgta aagccattaa
     3241 atcccaatta cgaaatcacc taccgcgact caaccaaact cttcaagcaa agccacaccg
     3301 tccacgcaaa gaatgtcatc ttctctgctg gtgtgatggg cacgatgaaa ctgctgctg
//
LOCUS       DAOREF010000261         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18185_length_3358_cov_3.579776, whole genome shotgun sequence.
ACCESSION   DAOREF010000261 DAOREF010000000
VERSION     DAOREF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18185_length_3358_cov_3.579776"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKI33_06600"
     CDS             complement(<1..418)
                     /locus_tag="PKI33_06600"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNM36711.1"
                     /translation="MNPVSKRSVIAIIIALLIVSGLALAGSQGGKTVNGFPIFAICVA
                     VAIGIQWVAFLPAFAMQTETFYDLTGSLSFLTTATVAILLSKASDIRSLLLAGMIALW
                     ALRLGTFLFTRVRAAGEDRRFREIKPNFARFMLTWTL"
     gene            complement(581..1012)
                     /locus_tag="PKI33_06605"
     CDS             complement(581..1012)
                     /locus_tag="PKI33_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36712.1"
                     /translation="MFYSFSVLFFVLGLVTAFPAIFRLAKMEKIRKNGVSTFGAIRSA
                     ESDLGWRMTGALGRVTHPLIEFQVNEQKHIIQVNDNRGFLAPRYDRGDTLEVIYDKDM
                     PWKAYIKQEWLIARKDLWKAAAEIVLAVVLWETGVYLGMPL"
     gene            complement(1063..1908)
                     /locus_tag="PKI33_06610"
     CDS             complement(1063..1908)
                     /locus_tag="PKI33_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNM36713.1"
                     /translation="MDSLSERRKAIFYLAITAILWSSSGLFVKILDWHPVAILAGRSF
                     FAAIVFLIYLRRIPTRFGLWQLLAAAMFILTQFLFITSTKMTTAANSIFLQYTMPIYV
                     VLLAYWFLGEKPSRTDWIAMLVILIGLALFFGDELSTEGFYGNLLAILSGVTGAIMMV
                     SFRAQKDGNPAESNLIAFLVTATFGFPFIMKETWTVNSILILAFLGIFQIGVAFIFFS
                     KGIKHIPALEANLIGTLEPVLNPIWVFLFYGESMGKFALIGGLIVLSGVILSAVGSAK
                     AAQNH"
     gene            2030..2971
                     /locus_tag="PKI33_06615"
     CDS             2030..2971
                     /locus_tag="PKI33_06615"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNM36714.1"
                     /translation="MKTGIVGSGFVGATAAYALVMRGVGRRVVLVDMNKARALAEADD
                     ILHAVPFAHQLEVTAGDYEDLADCKVVVVSAGVGQKPGETRLELLGRNAKVFEQVIPN
                     ILTHAPNAILLIATNPVDVMTHIAAHYAAKMGVPSNRVIGTGTTLDTARMRTLLGRHL
                     KVDSQHIHAYVLGEHGDSELLPWSLVTVGGIPLDEFCSDDDISICPDDYEDIDMRVRR
                     AAYRIIDGKGATYYGIGSAIARIVEVIQQDERSILTVCTPMPDVAGVKDVTVSLPNLI
                     GGEGIIQTFFPNLDPNEMDALRHSASVVRSAIEQLKL"
     gene            complement(3016..>3358)
                     /locus_tag="PKI33_06620"
     CDS             complement(3016..>3358)
                     /locus_tag="PKI33_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNM36715.1"
                     /translation="WHYDKKVFVLSSRRLDIPPELSEKVETLHQSPRELIRELERHGV
                     RHIYLDGGVTIQRFLREGLVDEMIITTIPILIGEGLPLFGQVGKDINLELLDVSSFSN
                     GLVQKKYKVLA"
BASE COUNT          889 a          830 c          837 g          802 t
ORIGIN      
        1 gcagggtcca ggtcaacatg aagcgtgcaa aattcggctt gatttcacga aagcgacggt
       61 cttctccggc ggcgcgaact cttgtgaaca agaaggtccc caaccgcaat gcccagagag
      121 caatcattcc ggctaacaac aaagatcgaa tatcacttgc cttgctgagc aagatagcaa
      181 ccgtggctgt agtgagaaaa gaaagactcc ccgtcaggtc gtaaaacgtt tcggtctgca
      241 tggcaaaggc gggcagaaac gccacccatt gaatgccaat tgccaccgca acacagatgg
      301 caaagatagg aaagccgtta acggtctttc cgccttggct tccggcaagt gccagcccag
      361 atacgatcaa taaagcaatg atgatggcga tcacagaacg tttgctgaca gggttcatgg
      421 ggttcctcga ttaataaata ttgaagcaat tgtagctgaa attttgagga tatgaaaccg
      481 gctctttttg caacttgggc ggatgtcttc gtttcaagcc ttctatttcc cgcaggctct
      541 ataacggcat acccagatac acccctgttt cccgcaggct ctataacggc atacccagat
      601 acacccctgt ttcccataac actaccgcca acacgatctc tgccgcggct ttccacaaat
      661 ctttgcgggc gatgagccat tcttgcttga tgtatgcctt ccaaggcatg tctttgtcgt
      721 agatcacttc aagcgtgtcg cccctgtcat aacgaggtgc gagaaacccg cgattgtcat
      781 tcacttgaat gatgtgtttc tgctcattca cctgaaactc gatcagtggg tgagtcactc
      841 tccccaaagc gccggtcatg cgccaaccga ggtcactttc tgccgagcgg atcgcgccaa
      901 aggtgctcac accgttcttg cggatctttt ccatttttgc gagccgaaat attgcaggga
      961 atgcggtaac caaaccgaga acgaaaaata aaacagagaa gctatagaac atcgtaagtc
     1021 tcccatgcca taacttctcc gaattttact gcgattttaa atttagtgat tttgggcagc
     1081 cttggcactt ccaaccgcgc tcaaaatcac accgcttaaa acgatcaacc cgccgatgag
     1141 cgcaaacttg cccatgctct cgccatagaa caaaaacacc caaatgggat taagaaccgg
     1201 ctcaagcgta ccgatgagat tcgcctccaa tgccgggatg tgcttgatgc ccttggagaa
     1261 aaagataaac gccactccga tctgaaaaat gccgaggaaa gcgaggatca gaatgctgtt
     1321 caccgtccac gtttctttca tgatgaaggg aaaaccaaat gttgcggtga caagaaaagc
     1381 aatgagattg ctctctgctg ggttgccatc tttttgcgcg cggaaggaaa ccatcatgat
     1441 cgcgccggtc acaccgctga ggatggcaag taaattgcca tagaaccctt ctgtgctgag
     1501 ttcatctccg aaaaataatg ccagcccaat caagatgact agcatcgcga tccaatctgt
     1561 gcgcgaaggt ttttcaccga ggaaccaata ggctaacaaa accacataaa tcggcatggt
     1621 gtattgcaaa aagatcgagt tcgccgccgt ggtcatcttg gtagaggtga tgaacaggaa
     1681 ctgggtcagg atgaacatgg cagctgccag taactgccac agcccaaagc gagtcgggat
     1741 gcgccgcaaa tagatgagga aaacgattgc ggcgaagaag ctgcgtccgg caaggatggc
     1801 gaccggatgc caatccaata ttttgacaaa aagtccgctt gagctccaaa gaatagcggt
     1861 gatagcgaga taaaaaatcg ccttacggcg ttctgaaaga gagtccactg agttcctcac
     1921 aaggggtaga ttggtgccga ttatacgctt tatcttggat ggagttctag taaaatcatt
     1981 tcgttattgg ttctgagcta ttaaatttta acgatagagg agtttaacaa tgaagacagg
     2041 aattgtaggc agcggctttg taggcgccac tgctgcgtat gccttggtga tgcgtggagt
     2101 ggggcggcgc gtggttttgg tggatatgaa caaagcgcgt gccttggcag aggcagatga
     2161 tattttgcat gctgtgccat ttgcacatca gttagaggtg actgccggcg attatgaaga
     2221 ccttgctgat tgcaaagtgg tggtggtttc tgcgggggtg gggcaaaagc caggcgagac
     2281 ccgtttggag ttattggggc gaaatgcaaa agtgtttgaa caagttattc ccaatatttt
     2341 gacacatgcc cccaatgcga ttttgttgat cgcgaccaac cctgtggatg tgatgacgca
     2401 catcgcggct cattatgccg ccaagatggg tgtaccatct aaccgcgtga tcggcaccgg
     2461 caccactcta gacacggctc gtatgcgcac cttgctggga cgtcacctca aagtggactc
     2521 tcaacacatc catgcttatg tgcttggcga gcatggcgac tcggaattgc tgccatggtc
     2581 gcttgtcacc gtgggtggaa ttccgctcga tgaattctgt tcagatgacg atatctcgat
     2641 ctgccccgat gattatgaag atattgatat gagggtccga cgagcggcgt atcgcatcat
     2701 cgacggcaaa ggcgcgacct attacggtat tggcagcgcc attgcccgca ttgtggaagt
     2761 gattcaacaa gatgagcgat ccattctcac ggtttgtacg cccatgcccg atgtggcggg
     2821 cgtcaaagat gtgaccgtct ccttacccaa cttgatcggc ggtgagggca tcatccaaac
     2881 gttcttcccc aatttagatc cgaacgaaat ggatgccctc cggcacagcg ccagtgtggt
     2941 ccgctcggcg atagagcaat tgaaactcta atcttgaatc aaaagagcgt gatgttcatc
     3001 acgctctttt gatttttagg caagcacttt atatttcttt tgcacaagcc cgttcgagaa
     3061 agaactcaca tccaacaatt ccaaattaat gtcttttcct acttgcccaa acagaggcaa
     3121 gccttcaccg atcaaaatag ggatcgtcgt gataatcatt tcgtccacca acccttcgcg
     3181 caaaaaacgt tggatggtga cgccgccatc gagataaatg tgacggacac catgccgctc
     3241 cagttcgcgg atcaactctc ggggagattg gtgaagcgtt tcaacttttt cgcttaactc
     3301 tggagggata tccaacctac ggctggaaag cacgaagacc tttttatcgt aatgccaa
//
LOCUS       DAOREF010000262         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18207_length_3355_cov_5.164242, whole genome shotgun sequence.
ACCESSION   DAOREF010000262 DAOREF010000000
VERSION     DAOREF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18207_length_3355_cov_5.164242"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKI33_06625"
     CDS             complement(<1..286)
                     /locus_tag="PKI33_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNM36716.1"
                     /translation="MANVHQFDVVVVGAGGAGLMAGLYASRNAKTAVISKLYPTRSHT
                     GAAQGGISAALGNYEEDKPEWHMYDTVKGSDYLGDQDAIEFMTTEAIDAVL"
     gene            complement(368..808)
                     /locus_tag="PKI33_06630"
     CDS             complement(368..808)
                     /locus_tag="PKI33_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36717.1"
                     /translation="MATVNKSRRLSIKARGLTNFESIMWLFTRLSALAMYALILFGLL
                     GALVMGARNNMNFADVMRWAFMPNVTHVQSTDLASTDAWATPLWKLTASALLLVATAH
                     GVHGLVVIADDYIAGETGRKIVRFFSIVVMVGWSLMGLWLIWTS"
     gene            complement(810..1184)
                     /locus_tag="PKI33_06635"
     CDS             complement(810..1184)
                     /locus_tag="PKI33_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36718.1"
                     /translation="MGLKRDVSLSTGLRYRGPAGYLTYILHRIGGLGMGIFITLHILA
                     SFVGGRFGGALNSIYESWIFQIFIFFCVLFHAINGLRITILDLFPKLLEHQTQAIWIE
                     WAVFIPVYGISVFVILKTALGG"
     gene            1516..2013
                     /locus_tag="PKI33_06640"
     CDS             1516..2013
                     /locus_tag="PKI33_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNM36719.1"
                     /translation="MSDSHEVSRRDFIKVTTGIVGGLIGTMIGLPSIFYLIDPALQED
                     AKEAWIPIGKFEDMQVGVPYPFSFTQVQVNGWERTASSFGGYAIRKSDNPDELLILNS
                     RCTHLSCTVNWSAEAQAYICPCHDAKFGMEGEVLDGPPPRPLDIYEEHRITADGIIEI
                     FFKGA"
     gene            2016..2675
                     /locus_tag="PKI33_06645"
     CDS             2016..2675
                     /locus_tag="PKI33_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNM36720.1"
                     /translation="MWKTIYTWLDERIGLNDLYTTMLDRPEPKTTWWNTLGSASMFLF
                     ILQGLTGIFLTVYYTPSPDHAYDSINYIMEGVAFGWLIRGIHHWGATLMVVLVFIHML
                     RVFYTASFKYPRELTWLIGIGLFLTTLGMGFTGYLLPWNQKSYWATTVGTQIAGTVPF
                     LGDFILKALRGGPDLSALTLQRFFSAHIWIIPATLAALMGIHLFLVIKHGESHWPQKE
                     D"
     gene            2679..>3355
                     /locus_tag="PKI33_06650"
     CDS             2679..>3355
                     /locus_tag="PKI33_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36721.1"
                     /translation="MNDETKKKINARYERELDKGERFWPDTIFRDLIMSLGIFILLIL
                     LASFVGVPAEPKADPSDTSYIPRPEWYFLFLFKFLALYGQLPVIGKIEWLATVLVPGI
                     AIAILTLMPFIEKSPYRYYSKRVLPISIMTIMVTGIVMLTLMSEVPTVSEDGSTLLGT
                     LQTVVGIFIPLTAYILFFAFKNNTRLMLWTVSLAAVSMILISGTVLALAPEKEAEEST
                     VATTLPD"
BASE COUNT          868 a          986 c          714 g          787 t
ORIGIN      
        1 cgagcaccgc atcgatcgct tcggtcgtca tgaattcgat cgcgtcctgg tcaccaagat
       61 aatcggaccc tttgacggtg tcgtacatat gccattcggg tttatcttct tcgtaattgc
      121 ccaacgctgc agagatgcca ccctgcgccg cgccagtgtg tgaacgcgta ggatacagtt
      181 tgctgatgac agcggtcttg gcattgcgcg aggcatacaa acccgccatc aaaccggcgc
      241 cgcctgctcc caccaccacc acatcaaatt gatgaacatt cgccatgaaa tattgctcct
      301 gaaaaattac ggacgataaa cgatggatga tagaccatgg tctatcatct actgtccatc
      361 atccatttta cgaagtccag atgagccaca accccatgag gctccatcca accatcacta
      421 caatgctaaa gaaacgcacg atcttgcgcc cggtctctcc ggcaatgtaa tcatctgcga
      481 tcacaaccag accatgcacg ccgtgagccg tagccaccaa gagcaaggcg ctggcggtca
      541 acttccaaag cggagttgcc caggcatcgg tgcttgccag atcggtgctt tgtacatgag
      601 tcacattcgg catgaatgcc cagcgcatca catcggcaaa gttcatattg ttgcgagcac
      661 ccatcaccag agcaccaagc aacccaaaga ggatcaaggc atacatcgcc aaagcagaga
      721 ggcgtgtgaa cagccacata atggattcaa aattggttaa tccacgggct ttgatggaga
      781 ggcgtcgcga tttattgaca gtagccatgt taccctccca acgcagtctt caaaataacg
      841 aaaaccgaaa tgccatagac cggaatgaat accgcccact cgatccaaat ggcttgcgtt
      901 tgatgttcca aaagttttgg gaaaagatcc aaaatggtga tgcgcaagcc gttgatggca
      961 tggaacaaca cacaaaagaa aatgaagatc tggaagatcc agctctcata aatactattt
     1021 aatgccccac caaacctgcc gccgacaaat gaagcgagga tgtgcaaggt aatgaaaata
     1081 cccatgccca acccgccaat gcggtgcagg atataagtca ggtaccccgc tggtccgcga
     1141 taacgcaaac ctgtggagag gctgacatcc cgtttcaagc ccatgattgc ctccgtggtg
     1201 aattttgtta caagcgcata ttctactcgc tatctcttaa aagtttaagt ggaaagatgg
     1261 acaaaggggt atttccatcc ttttaatact gaaaagactc ttctaatacc gcacggctat
     1321 cgattttaat aaaactgcct gctcagccga aatagcgaaa cgtgacaaaa atcctgctaa
     1381 tttcattcct gccgtcctat aatgaaggga acagagaccc ttataatcca attccaatag
     1441 ctcttaacgc cgcatcgttc cacctttatc cactagtcga atggcaagcg caatatattc
     1501 cccttggagg cgcacatgtc tgattcacac gaggtatccc gccgggattt catcaaggta
     1561 acgacgggca tcgttggagg tttgatcggc acaatgatcg gtcttccctc cattttctat
     1621 ctgatcgacc ccgcattgca ggaggatgca aaagaagcgt ggattcccat cggtaaattc
     1681 gaagatatgc aagttggcgt accctacccc ttctcgttca cccaagtaca ggtcaacggg
     1741 tgggagcgaa ctgccagttc ctttggcgga tacgccatcc gcaaatcgga taaccccgat
     1801 gagttgctca tcctcaacag tcgttgcacg catctttcct gcaccgtaaa ctggtcagct
     1861 gaagcacagg catatatctg cccctgtcac gacgccaagt tcggcatgga aggcgaagtg
     1921 ttagatggac ctccacctcg cccgctcgat atctatgaag aacatcgcat cactgcagat
     1981 ggcatcattg agatcttctt caagggagca taaccatgtg gaaaacgatc tacacctggc
     2041 tcgatgaacg tatcggtctc aatgacctgt ataccaccat gctcgaccgc cctgaaccca
     2101 aaacaacctg gtggaacacg ctcggctcag ccagcatgtt ccttttcatt ttacaagggc
     2161 tcaccggcat cttcctcacc gtgtactaca ccccctcccc agatcacgcc tacgactcca
     2221 tcaactacat catggaaggg gtagcgtttg gttggctcat ccgcggcatt catcattggg
     2281 gcgcaaccct catggtggtc ttagtcttca tccacatgct gcgagtgttc tacaccgctt
     2341 ccttcaaata cccccgcgag ctgacctggc tcatcggcat cggcttattt ctcaccactc
     2401 ttggcatggg cttcacaggt tatttgctgc catggaatca aaaatcctat tgggcaacca
     2461 ccgtcggcac acaaattgcc ggaaccgtcc ccttcctcgg tgatttcatc ctcaaagcct
     2521 tgcgcggcgg acctgacctc agcgccctca cattacagcg cttcttctcc gctcatatct
     2581 ggatcatccc tgccactcta gcggcattga tgggcatcca tttattcctc gtcatcaagc
     2641 acggcgaatc gcactggccc caaaaggaag attaagccat gaacgacgaa accaaaaaga
     2701 aaatcaacgc acgctacgaa cgcgaactcg acaaaggcga acgcttctgg cccgatacca
     2761 tcttccgcga cctcatcatg tcgctgggaa ttttcatcct actgattctc ctagcatcct
     2821 ttgtcggagt ccccgccgaa cctaaagccg acccaagcga tacctcctac atcccgcgcc
     2881 cagagtggta tttcctcttc ctcttcaaat ttctcgcctt gtacggtcag ttacctgtta
     2941 tcggcaaaat tgaatggctc gccactgtgc tcgtgcccgg catcgccatc gccattctga
     3001 ccctcatgcc cttcatcgaa aagagtccat accgctatta cagtaaacgt gtattaccca
     3061 tctccatcat gaccatcatg gtcactggca tcgtgatgct caccttgatg tctgaagttc
     3121 caaccgtttc tgaagatggc tccacactac ttggaactct tcaaactgtt gtagggatat
     3181 tcatcccgct gaccgcatac atcctcttct tcgctttcaa aaataacacc cgcctcatgc
     3241 tctggaccgt aagcctggct gcggtttcca tgatcctcat ctcagggaca gttcttgctc
     3301 tcgctcccga aaaagaagcg gaagaatcca cagttgccac caccttgccc gatca
//
LOCUS       DAOREF010000263         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18215_length_3354_cov_5.026675, whole genome shotgun sequence.
ACCESSION   DAOREF010000263 DAOREF010000000
VERSION     DAOREF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18215_length_3354_cov_5.026675"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..537)
                     /locus_tag="PKI33_06655"
     CDS             complement(85..537)
                     /locus_tag="PKI33_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNM36722.1"
                     /translation="MKFLLERSRYLTLIGIISLLIASFAAFAWGTIKTVTTTLLVIET
                     LGKDSSITIELIELIDIFLIATTILIFSASLYELFIGKLDMPEWMLAHDLYELKAKLS
                     SMIVLVMAVKYLQKLIEIKDGTELLQRGIAVAIVSAALIAFGYFGKKD"
     gene            complement(631..1461)
                     /locus_tag="PKI33_06660"
     CDS             complement(631..1461)
                     /locus_tag="PKI33_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM36723.1"
                     /translation="MHRERVSENVFWFQSEVYAQVTAGVIIGPQWAVVIDTLALPEET
                     LSMREFIEHELGVQVRYVINTHYHADHCWGNCFFPGAVVIGHSKCRQYLVEDGAESLE
                     NAQKQNPNLRQVKIVPPHITFETGEMTLRVGKKNLIISTSPGHSDDGISVLVEEDRVL
                     FAGDAFMPLPFIVDGDADDMLNSIKKIGKMGLENIIQGHGDIILRGEIDAAVRENVNY
                     LNNIKKAVKSGGKRKNADEYLEEIAIEDCGKSRVYLGGLAETLHRRNLRALHRQMFGE
                     "
     gene            1659..2588
                     /locus_tag="PKI33_06665"
     CDS             1659..2588
                     /locus_tag="PKI33_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36724.1"
                     /translation="MVRKLSVVLLILFQISCSAATPEPTATFTPLPPTATVTPTPAPT
                     ATQLPASPMPDAITYEGNGFRFAVPDTLDVDMQKNAVGIYDKSGTLIISFTRVPYDES
                     TDTLEDVINDYLAEISTRGGGKFTQSEATSITVAGAEGLSVELSGTLFERPIEGRAIA
                     VVPDKNSVLFGMGVSNLVSDETLWQSTGSLIFQGLLDSLEFVDVQIAAQCTVSTDKSY
                     GYTETNPIKVGGDFIDGPARERAYLDNLLGPNGEALSYEREGSINSGDTILDIYRVTG
                     NGLNVTLYLDEYTYSPLLAPVGLTCVNEFISAP"
     gene            complement(2633..>3354)
                     /locus_tag="PKI33_06670"
     CDS             complement(2633..>3354)
                     /locus_tag="PKI33_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM36725.1"
                     /translation="ELTKAGIKIKTGIKFESITATKDKVKVVLPDGTLEVDQALIATS
                     FTPNSKGLGLEAVGVKISERGFIEINEKMQTNVPNIWAIGDVTGKLMLAHVGSAMGIV
                     AAEHIGGHETITLNYEMMPRATYCHPQVASFGLTEAQAKERGYNIKVGRFPFQANGKA
                     LGLGDYAGFVKIITDEKYGEILGAHMMGPEVTELLPELTLAQMMELTPHEIARNVHAH
                     PTLSETLMEAAHGASGTPIHI"
BASE COUNT          786 a          786 c          850 g          932 t
ORIGIN      
        1 tacaagccgc aaagtgagtc caagccgcct tgttgaaagg gcggaagtcc cgtcattaag
       61 cggcttgacc ttctcatggt gccatcagtc ctttttgccg aaatatccga aggcgatcag
      121 cgccgcagag actatggcaa cggcaatgcc gcgttggagg agttcagttc catctttgat
      181 ttcgatcagt ttttgcaaat acttgactgc cattaccagc acgatcatgc tgctgagttt
      241 ggctttgagt tcgtacaggt catgtgccaa catccattcg ggcatgtcta atttgccgat
      301 gaagagttcg tacaggctgg cagagaagat cagaatggtg gtggcgatca ggaaaatgtc
      361 gattagttcg atcaactcga tggtgatgga actgtctttg ccgagggttt cgattactaa
      421 cagtgtagta gtcactgttt tgatggtgcc ccaggcaaag gcggcgaaag aagcgatcaa
      481 tagagagatg atgccgatca aagttaggta gcgtgaacgt tccagtaaaa atttcatgtt
      541 gttaatcctc ttgtcataat gtataaggct ctagccacta acaaagatgt tgcagacccg
      601 gcttgcatct gcaacatcta gtgtattcgt ttactctccg aacatttggc ggtgtaaggc
      661 tcgcaggttg cgcctgtgca gggtctctgc cagtccgccg agatagacac ggctcttgcc
      721 acaatcttca atggcgatct cttcgagata ttcatcggca ttcttgcgtt tgccacccga
      781 cttgactgct tttttgatat tgttgaggta gtttacgttc tcacgtacgg cggcgtcgat
      841 ctcacctcgc aggatgatat cgccatgacc ttggatgata ttttccaagc ccatcttgcc
      901 gatcttctta atggagttca acatgtcatc ggcatcgcca tctacaatga agggcaatgg
      961 catgaaagca tcaccggcga agagcacacg gtcttcttcg acgaggacgg aaatgccatc
     1021 gtcactgtga ccgggggaag tggagatgat gagatttttc ttacccacac gtagggtcat
     1081 ttcaccggtt tcaaaggtga tgtgcggcgg cacgatctta acctgacgca gattagggtt
     1141 ttgcttttga gcattttcca atgattcagc gccatcttcc accagatatt ggcggcattt
     1201 cgaatgaccg atcacaacag cgccggggaa gaaacagtta ccccagcagt ggtcggcgtg
     1261 ataatgggtg ttgatcacat aacggacctg tacgcccaat tcgtgctcga taaattcgcg
     1321 catggagagg gtctcttcgg ggagagcgag ggtatcgatg acaactgccc attgcggacc
     1381 aataatgaca cccgcagtga cctgcgcgta gacttcgctt tggaaccaaa atacgttttc
     1441 agaaactcgt tcgcggtgca tagttttacc ttcttcactg gttcgccggt ttgctatccc
     1501 atactggagc gaagccattt gtgtttagta ttcgttgtta atagcataca atcttaaaaa
     1561 tttcaagatg tatagacaaa tatggagatt tcttcagatt tgagttatat tctgacaaga
     1621 cttgtgcctg tcccttcacc caaaaaggag gattctagat ggttcgaaaa ctttctgttg
     1681 ttttgttgat cctgttccaa atctcatgta gtgcggctac ccccgagcca acggcgactt
     1741 ttaccccatt gccgcccacc gctactgtca cacctacgcc tgctccgacc gctacacagc
     1801 ttccggctag ccctatgcct gatgcgatta catacgaagg taacgggttt cgctttgcgg
     1861 tacctgatac cttagatgtg gatatgcaaa agaatgccgt ggggatttat gataaatctg
     1921 gcactttgat catctctttt acccgagtgc cgtatgatga atctaccgat acattggaag
     1981 atgtgatcaa tgattatctg gcagagattt ctacgcgtgg tggcggcaag ttcacccaaa
     2041 gcgaggcgac ttcgatcaca gtggctgggg cagaggggct ttctgtggaa ctaagcggca
     2101 ctctttttga gagacccatt gaaggcagag ccattgcggt ggtgcctgac aagaattcgg
     2161 tgctatttgg tatgggagtc tcaaatttag tcagtgatga gacgctatgg caaagcactg
     2221 gttcattgat ctttcagggg ctattggatt cactggaatt tgtcgatgtg caaatcgctg
     2281 cgcagtgtac tgtcagcaca gataaaagct atgggtatac agaaaccaac ccgatcaagg
     2341 tcggaggcga ttttatcgat ggacctgctc gtgaacgagc ctatctcgat aatttgttgg
     2401 gtccgaatgg tgaggctttg tcttatgaac gtgaaggctc tataaatagc ggtgacacga
     2461 tcttggatat ttatcgtgtt acaggcaacg gtttgaatgt aaccttgtat ttggatgaat
     2521 acacatattc gcctctcctt gcaccggtgg ggttgacctg cgtgaatgag tttatctctg
     2581 ccccgtaatg tgaataaaaa actccgaagc ttcatcgctt cggagttttg ttttagatgt
     2641 gaatgggagt tccgcttgcg ccgtgtgcgg cttccatgag ggtttctgaa agcgttgggt
     2701 gcgcatgaac gttacgagca atctcgtgcg gagtcagttc catcatttgc gcgagggtca
     2761 gttcgggcaa cagttcggtc acttcgggtc ccatcatatg agcaccgagg atctcgccat
     2821 atttttcatc ggtgatgatc ttgacgaagc ctgcataatc acccagccca agcgctttgc
     2881 catttgcctg gaaggggaag cgcccgacct tgatgttgta cccgcgttct tttgcttgtg
     2941 cttcggtgag cccaaacgaa gcaacctgcg gatgacaata ggtagcgcgc ggcatcattt
     3001 cgtaattgag agtgatggtt tcatgtccac cgatgtgttc agcagccaca atacccattg
     3061 ctgagccaac atgtgccaac atcagtttgc cggtcacatc gccgattgcc caaatgttgg
     3121 gaacattggt ctgcatcttc tcgttgattt caataaatcc acgttcgctg atcttgacgc
     3181 ccaccgcctc aagtcccagc cctttgctgt tcggcgtgaa ggaagtggcg atcaacgcct
     3241 gatccacttc caacgttcca tcgggcagca caactttcac cttatctttt gtagcggtaa
     3301 tgctttcgaa cttgatgcca gtcttgatct tgattccagc tttggtgagc tctt
//
LOCUS       DAOREF010000264         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18229_length_3353_cov_2.558217, whole genome shotgun sequence.
ACCESSION   DAOREF010000264 DAOREF010000000
VERSION     DAOREF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18229_length_3353_cov_2.558217"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1311)
                     /locus_tag="PKI33_06675"
     CDS             complement(211..1311)
                     /locus_tag="PKI33_06675"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNM36726.1"
                     /translation="MEEIPAIDLKRQYQSIRKEIDEAYARVMSGGSFILGTEVAAFEK
                     EFAEYCGVSYAIGVASGTEALQLALMACGIEENATVLTTAHTAVATVGAIEASGAYPK
                     LLDIDLSRYTLDPAELEKSINDQTCAIIPVHLYGCSANMRPILDVAKEKKLFVIEDAS
                     QAHGALYFGHKVGAMGHAAAFSFYPTKNLGAFGDGGAVLTNDSGIAERVRLLRQYGWK
                     EHYVSSVKGINSRLDELQAAILRVKLRHLDEWNQRRNQLADLYFELLADTDLVLPLRP
                     DDCEHVFHQFVIRHPRRDALREYLRSKGIHTLIHYPVPIHLQPAYKNLGLNPGDLPKA
                     EQAAREMLSLPIYPELTEREVRRVAEAVRSFF"
     gene            complement(1329..2321)
                     /locus_tag="PKI33_06680"
     CDS             complement(1329..2321)
                     /locus_tag="PKI33_06680"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM36727.1"
                     /translation="MIDVNSSYFQKNVLITGGAGFIGSNLASTLLGLGATVTIFDNFE
                     SDSGANRFNLEGIDNHIRLIQADIRDEVAMKDAVQGQDFLFNLAGQMSHIGSMQDPFK
                     DLYVNTVGQLNLLEVCRQHNPKIRVVYAGTRQVYGRPKYLPVDEKHLLTPLDNNGVSK
                     RAGEMYHIVYHHVHGMATCVLRMTNTYGERMRIKDDKLTFIGWWIQRILTGNEIQIFG
                     DGKQIRDFNHVSDVIRAMLLCAVNPAADGKIYNLGGEPISLIDLAALLVEVNGSGAFT
                     TVPFPENRKRIDIGGYYGDYSLIKNEIGWQPQVSLKDGLAQMLAFYREHKSYYL"
     gene            complement(2318..3052)
                     /locus_tag="PKI33_06685"
     CDS             complement(2318..3052)
                     /locus_tag="PKI33_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM36728.1"
                     /translation="MSQKRISSISVVFPAYNDGGTIASMVAAASIACKQVTNDFEIIV
                     VNDGSSDYTASILEELTHKYPQLRVLTHHSNQGYGATLKTGFTAAQKDWVFYTDGDSQ
                     YNPLELVNLVNALQEGVDLVNGYKLTRNDSWIRIVIGRAYHYLVKFLFNIPIRDVDCD
                     FRLIPRNVLDEIDLKSVSGAICLEMVKKIADAGYVFAEVPVNHYSRKYGVSQFFVPWR
                     VLRTLQQIAQLYWMLVIRKEHLKNKK"
     gene            complement(3049..>3353)
                     /locus_tag="PKI33_06690"
     CDS             complement(3049..>3353)
                     /locus_tag="PKI33_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36729.1"
                     /translation="KHDQRVHTNRRYTSKQVAGLLEKSGLHVQHLSYANTFLFPLAVV
                     KRLSERFFPPDEDYSDLSLNTGMFNDFLKFILASEASLVTKYGLPFGLSVIAVGQK"
BASE COUNT          972 a          820 c          624 g          937 t
ORIGIN      
        1 caaacgatgg tactcaagag catcttcacg tgtgaattta gccatacagc ctccagaagt
       61 ttagtttgca actatcgata aaattatcgc acgactttac aggcaggaat agttgctttt
      121 gtcataattt ggggagttta gacagcccta ttatgtttta cccttattcg agaaaaagtt
      181 ccgcagaaag cctatcaatc cctcgcaggc ttagaaaaat gatcgaactg cttcagccac
      241 tcgccgtact tctcgctcgg tcagttcggg gtagatgggc aaagaaagca tttcacgtgc
      301 ggcttgttct gcctttggca aatcgccagg gttaagtccc agatttttat aagcaggctg
      361 aagatgaatc ggaaccggat aatgaatcaa agtatgaatc ccctttgaac gtaaatattc
      421 cctcaatgca tcacggcgcg ggtggcgtat cacaaattga tggaagacgt gttcacaatc
      481 atccgggcgc agcggtagaa caaggtctgt atctgccaat aattcaaaat ataaatctgc
      541 gagttgattt cgtcgttgat tccattcatc caaatggcgc aacttaactc ttaagatcgc
      601 cgcttgtaat tcatccaagc ggctgttaat ccctttgacc gaactcacat aatgttcttt
      661 ccaaccatac tgacgcaaaa gccgcacccg ctcagcaatg ccagagtcat tggtgagtac
      721 agcaccccca tcgccaaagg ctccaagatt ttttgtggga taaaaactaa atgcagcagc
      781 atgtcccatc gcgcccactt tatgtccaaa atacaaagcg ccatgggctt gcgacgcatc
      841 ttcaataaca aataattttt tttctttggc aacatccaaa attggtcgca tattggcaga
      901 gcaaccatac aagtgcaccg gaataatcgc acatgtttga tcgttgattg atttttccaa
      961 ctcagctgga tccagcgtat atctggataa atcaatatct aacaattttg gatatgctcc
     1021 gctcgcctct atcgcaccga cagttgccac tgctgtgtga gcagtcgtca acactgtggc
     1081 attttcttct atcccacaag ccatcaacgc caattgcaaa gcttctgtcc ccgaagcaac
     1141 accaatcgca tatgaaactc cacaatactc tgcaaattct ttttcaaatg ccgcgacttc
     1201 ggttcctaaa ataaagcttc caccagacat cactctggca taggcttcgt caatttcttt
     1261 acgaatcgat tgatattgtc gtttaaggtc aattgctgga atttcttcca ttacacacca
     1321 tagccattct acaaataata tgatttatgc tctcgataaa aagcaagcat ttgcgctaaa
     1381 ccgtctttga gtgagacttg tggctgccag ccaatttcgt tttttatcaa tgagtaatcg
     1441 ccatagtacc cgccaatatc aatacgtttg cgattctcag gaaaagggac agtagtgaat
     1501 gctccgctac cattgacttc aactagcaat gcagcaaggt cgatcaaact aattggttca
     1561 ccaccaaggt tatagatttt cccatccgcc gcaggattca cagcgcataa caacatggct
     1621 cgaattacgt cacttacatg gttgaaatct cgaatttgtt tgccatcgcc aaaaatctgg
     1681 atttcattcc ccgtcagaat acgttgaatc caccagccaa tgaaggtcag cttatcatcc
     1741 ttgatcctca tcctctcgcc ataggtattt gtcatacgca atacacaagt cgccatccca
     1801 tgcacatggt gataaacaat atgatacatc tcgcccgcac gcttgctcac tccgttgtta
     1861 tccaacgggg tcagtaagtg tttttcgtcc acgggcaaat attttgggcg accatatacc
     1921 tgacgagtcc cggcatatac aacgcgtatc ttcgggttgt gttgccggca aacttccaga
     1981 agatttagtt gccccactgt atttacatat aaatctttaa atggatcctg catgctgcca
     2041 atatggctca tctgcccggc aaggttaaaa agaaagtctt gcccttgaac agcatctttc
     2101 atcgctactt catcgcggat atccgcctgg atgagccgaa tatgattgtc gataccttca
     2161 aggttaaagc gatttgctcc agaatcggat tcaaaattat caaagatcgt aacggtagca
     2221 cctaaaccta gcaatgtgct cgccaaatta ctgccaataa aacctgcgcc acctgtaatc
     2281 aacacatttt tttgaaaata gcttgaattt acatctatca ttttttattt ttcaagtgtt
     2341 cttttcgtat gacaagcatc caatacaatt gagcgatctg ctgaagtgtg cgtaaaacac
     2401 gccaaggcac aaaaaactgc gagaccccgt actttctcga ataatggtta accggaacct
     2461 cagcaaaaac ataccctgca tctgcaatct ttttgaccat ctccaagcag atggcaccac
     2521 tcacactttt taaatctatt tcgtcaagca cgtttcttgg gataaggcga aagtcacaat
     2581 ctacatcccg aataggaata ttgaaaagaa acttcactaa atagtgatat gcccgcccaa
     2641 tcacgatacg tatccacgag tcattgcgcg ttaatttata cccattaacc aaatccacac
     2701 cttcttgtaa agcattgacc aagttaacca attccagcgg gttatattgc gaatcaccat
     2761 ctgtataaaa aacccagtct ttttgggcgg cggtaaaccc agtttttagt gttgccccat
     2821 acccctgatt cgaatgatga gttagaacac gcaattgagg gtatttatga gtcaattcct
     2881 caagtatgct tgctgtatag tctgagctgc catcattgac tacaataatc tcaaaatcat
     2941 ttgtcacctg tttgcaggca atactagcgg cagccaccat acttgcgatt gttccaccat
     3001 cgttatacgc cggaaaaaca acactgatgc tcgaaattct tttttgactc atttttgccc
     3061 cactgcaatg acgctcaaac cgaaaggcaa tccatatttc gtaactagcg atgcttcact
     3121 tgcaagaata aacttcaaaa aatcattaaa catccctgta ttcaaagaaa gatcagagta
     3181 atcttcatct ggaggaaaaa acctctcgct gagtcttttt actacagcga gagggaaaag
     3241 aaacgtattt gcataggaaa gatgttgcac atgtagcccg ctcttttcga gcaagccagc
     3301 aacttgtttg cttgtatagc gacggttggt atgaacccgc tggtcatgtt ttc
//
LOCUS       DAOREF010000265         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18259_length_3349_cov_4.637826, whole genome shotgun sequence.
ACCESSION   DAOREF010000265 DAOREF010000000
VERSION     DAOREF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18259_length_3349_cov_4.637826"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKI33_06695"
     CDS             complement(<1..232)
                     /locus_tag="PKI33_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNM36730.1"
                     /translation="MPDPIQLHLLKPGQSATITRVGGSSALRRRFIEMGIVRGETILI
                     ERNAPLGDPVEYFIKGYHLALRKEEAANIEVVL"
     gene            complement(254..907)
                     /locus_tag="PKI33_06700"
     CDS             complement(254..907)
                     /locus_tag="PKI33_06700"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNM36731.1"
                     /translation="MSVQPLSESTEMYLKAMVELGSRDVPISRLAERLSVTNVSANEM
                     MRRLAEQGLVTHTPYKGVTLTKKGKEVAVNVIRRQRLWECFLYDHLKIEWSKVYEFAC
                     SLEHATAPEVTEALAGFLNHPSTCPRGNPIPDEHGVFAPLKGRVISELKPGESATVLA
                     VSATDTEVLKYLQDKKILPGQELKFIEAAPFQGPLTVSVNGKEVALGLSLVDYVIVK"
     gene            complement(1052..1642)
                     /locus_tag="PKI33_06705"
     CDS             complement(1052..1642)
                     /locus_tag="PKI33_06705"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNM36732.1"
                     /translation="MKKNQRYQKQKQQDQVKLSAIVGGGLVLLGFALFLVFQKPYSAS
                     ASETTIPDSVVPMAVEFPAPNLVLENVKSGTEALTDYHGKVVLVNNWATWCPPCKAEM
                     PSLQKFYEAHSGDGFMIVAINAGDDRAPVEQFVKEYGLTFRVWLDPDGAALDAFRNAN
                     LPSSYVVDRTGIIRYAWTGEISYEMLEKYITPVIQN"
     gene            complement(1651..1860)
                     /locus_tag="PKI33_06710"
                     /pseudo
     CDS             complement(1651..1860)
                     /locus_tag="PKI33_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(1876..2319)
                     /locus_tag="PKI33_06715"
     CDS             complement(1876..2319)
                     /locus_tag="PKI33_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36733.1"
                     /translation="MEKLLNEQVIGQLKQAFAELSNPVQLLFFGKKENCEYCAETQQL
                     LTEISDVEPRLHLSIHDLNEDAALAAQYRVDKAPMIIVASKNGEKAIDLGVQFAGIPA
                     GYEFTTLVNDILLASKGTTVLGAATREFLQTLTKPLHLQVFVTPT"
     gene            2435..3040
                     /locus_tag="PKI33_06720"
     CDS             2435..3040
                     /locus_tag="PKI33_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM36734.1"
                     /translation="MNTSITNDFSIDALRNGDRAEFARLVDTYSSPIYRLGLKMLGNE
                     QDAEDILQNTFLNALTHLADFEGRSSLSTWLYRIAANEALMLIRKRKPNINIEDMQPE
                     DADDVPLPETFVDWSVLPEKELLSGESKKAIEAAVKKLPESLRMVFILRDVEDLSIKE
                     TAEILNLTEVNVKTRLLRARMALREQLSSYYAERTRRKETQ"
     gene            3037..3297
                     /locus_tag="PKI33_06725"
     CDS             3037..3297
                     /locus_tag="PKI33_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNM36735.1"
                     /translation="MTNHTHQNCQALLGSLSEYIDGELPPNLCKEIEKHLEGCDNCRI
                     VLNTTRRTIDLVQIPAEENVPDDVRERLFKRLNLDDYLKQQS"
BASE COUNT          933 a          798 c          720 g          898 t
ORIGIN      
        1 tgagcaccac ctctatattg gcagcttctt ccttccgtaa ggcaagatga taacctttga
       61 tgaaatactc aacgggatca cctaatgggg cgtttcgctc gatcaaaatc gtctcaccgc
      121 ggacaatgcc catttcaata aatcggcgac gtaaagcgct cgagccacct acacgagtga
      181 tcgtggcaga ttgaccgggt ttcaataaat gtagttggat ggggtcaggc atagggaata
      241 ctccaaaata gttttatttc acaatgacat aatctaccaa agacaaaccc agcgcaactt
      301 cttttccatt gacggatacc gtcagcggac cctgaaatgg cgcggcttca ataaacttaa
      361 gttcttgccc tggcagaatt tttttatctt gcaaatattt gagaacttct gtatccgtag
      421 cgctgactgc caatacggta gccgattcac ccggtttcaa ttcactaatt acacgcccct
      481 ttaaaggagc aaaaacacca tgttcatctg gaatgggatt gccacgcggg cacgttgagg
      541 ggtgatttag aaaaccagcc aacgcttcgg tcacttcggg tgcagtagca tgttcaaggg
      601 aacaagcaaa ttcataaact tttgaccatt cgatcttgag atggtcgtac aaaaagcact
      661 cccacagccg ttgacggcga attacattga cagccacctc tttgcccttt ttcgtcaggg
      721 tgaccccttt atacggggta tgcgtaacca atccctgctc cgcgaggcgt ctcatcattt
      781 cattggctga tacattcgtc acactcaggc gttctgcaag gcgagaaata ggaacatcgc
      841 gacttcccaa ctcgaccatg gcttttaggt acatttcggt gctttcactc aacggctgaa
      901 cagacataag gctaccctat taagaatgtt atggttcacc ttattattcc aaagagaccg
      961 tttttccgtc atggaaagag gtcttaaatt ccgtggatac atgtcataca taaaaaaacg
     1021 ccccgaatgg ggcgtttttt gtttattaga actaattttg aataaccgga gtaatatatt
     1081 tttcaagcat ctcgtagctg atctcccccg tccatgcata gcggataatc cctgtacggt
     1141 ccaccacata ggagcttggc aaattcgcat tgcggaaagc atccaacgca gctccatctg
     1201 ggtccaacca cacacgaaat gtcagcccat attctttcac aaattgctcc acaggagctc
     1261 gatcgtcccc agcgttgatg gcaacgatca taaaaccatc gccagaatgt gcttcgtaaa
     1321 atttctgaag cgagggcatc tctgccttac aaggcgggca ccatgttgcc caattgttta
     1381 ccaaaaccac tttcccgtga taatcagtca atgcctcagt tccactctta acattttcaa
     1441 gaacaaggtt tggagcagga aattcaactg ccatcggcac tactgagtca gggattgtgg
     1501 tctcagatgc cgaggcagag tatggctttt ggaaaacgag aaacaatgca aaacctagca
     1561 aaaccagccc gccgcctaca atagccgaga gcttaacttg atcttgttgt ttttgctttt
     1621 ggtatctctg attctttttc attacaaagc ctaattttgc attgcgcgct tgatctcggc
     1681 gaccatctga gcttcgccat acgctccaac cacaatgccc tttccggaat cgatcacagt
     1741 ttgcgggact ccctgaacgt ggaactggtc tgaaagttct gggaattcca tggcatcaat
     1801 cgcctcagct cggaccatgg ttgggttttc catagccatc tggtgtgcga gcaacacggc
     1861 tcgcggacag tgcggtcagg taggggtaac gaagacttgc agatgcagag gctttgtcag
     1921 cgtttgcaaa aactcacggg ttgcagcacc cagcacagtc gtcccttttg aggcgagcag
     1981 gatgtcatta acaagggtag tgaattcata tccagcaggg atgccggcaa attgcacacc
     2041 caagtcaatc gctttttcgc cgtttttcga ggcgactata atcatgggcg ctttatccac
     2101 acgatactga gcagcgagag ccgcatcttc attcagatca tgaattgaaa gatgaaggcg
     2161 cggttcaaca tcgcttattt cagttagaag ttgctgggtt tcggcacaat actcacagtt
     2221 ttcttttttg ccaaagaaaa gcaactgcac agggttagaa agctcagcaa atgcttgctt
     2281 taactgtcct atgacttgtt cattcaacaa tttttccatg gggtctccaa taatttcggg
     2341 tcactttttg gatgcataag acgtaataaa gttacaagaa cttttgtctg tgttgtatac
     2401 tctgcatgaa actttttgag ataaactaca tctaatgaac acatccataa caaacgattt
     2461 ttcaattgat gcccttcgta atggagatcg tgcagaattt gctaggctgg tagataccta
     2521 ttcatcaccg atctatcggc ttgggctgaa aatgctgggt aacgaacagg atgcagaaga
     2581 tattcttcaa aacaccttct tgaacgcact tactcacctg gctgatttcg aaggtcgctc
     2641 gagcctctcc acttggctgt atcgaatcgc tgcaaacgaa gctttgatgc tcatccgcaa
     2701 gcgcaagcca aacatcaaca ttgaagacat gcagcctgaa gatgcagacg atgtgcctct
     2761 accagaaacg tttgtggatt ggagcgttct gcctgaaaaa gaattgcttt ctggtgaaag
     2821 taaaaaggca attgaagccg ccgttaagaa acttcccgaa tcacttcgaa tggtattcat
     2881 tctccgtgat gtagaagacc tttcgatcaa agaaacggct gagattttaa atttgactga
     2941 agtgaatgtg aaaactcgtt tattgcgtgc ccgtatggct ttacgtgaac aactatcctc
     3001 ttattatgca gagcggaccc gcagaaagga aacccaatga ccaaccatac ccatcagaat
     3061 tgccaggcgc tactaggctc tttatctgaa tatattgatg gtgaactgcc gcccaatttg
     3121 tgcaaagaga ttgagaagca ccttgaaggt tgtgataact gccgcatcgt actcaatacc
     3181 actcgccgta ctattgacct agtacaaatt cctgcggaag aaaatgttcc agatgatgta
     3241 agggaacgct tatttaaacg acttaaccta gatgattact taaagcaaca aagttaattg
     3301 ccactataat cagatgctat gaaaacaatt caaaattttg ctttcctgg
//
LOCUS       DAOREF010000266         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18369_length_3337_cov_3.353138, whole genome shotgun sequence.
ACCESSION   DAOREF010000266 DAOREF010000000
VERSION     DAOREF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18369_length_3337_cov_3.353138"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKI33_06730"
     CDS             <1..841
                     /locus_tag="PKI33_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNM36736.1"
                     /translation="DSTQLIYLRSRYYNPADGRFQSRDTWSGDYNRPLSLNRWMYVEG
                     NPVKYTDPSGFCPEPRTDEDPCWGLLLQIESRYSFVDLESELSKFNFWTYEELLYIQK
                     DLERFDQASKVDLGTLYPSSVKLKRILANDNKACAETNRYFVKNSSIVFYNSFDKLCG
                     EGVLVHELGHYLDGINQRKYSKEFEEYVGAFTFLWWYNLGQENPPIYGSGYPPNRRED
                     FAESLEEYVLLYTNYSGPDIGIVPGEKRWKFIEFVLDTGQLSINKEQYLCLPSYGKLI
                     NLN"
     gene            859..1509
                     /locus_tag="PKI33_06735"
     CDS             859..1509
                     /locus_tag="PKI33_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36737.1"
                     /translation="MKIKTIILRILLIPLPLILVLTGCKDSKGTVLVGTRDCEPPCWL
                     DIMPGVTKSGDMIEILKKKEQAKEGFLTLLDGGIIRWQSLDGYNSYVYPSENEVVTRI
                     EVDLRSNSIKLDDLISIFGEPLTLDIGKVEDGYFRSTVFYPNKGLAFVISGDEIDITT
                     SDLKFLFNPDMAVLKGIFFSPMEDISTMINLLYSSNAKPSVLLNIQNWTGYGIYYK"
     gene            complement(1649..2497)
                     /locus_tag="PKI33_06740"
     CDS             complement(1649..2497)
                     /locus_tag="PKI33_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNM36738.1"
                     /translation="MNSPEWKTCILGYVNHRPRSWRESERLFHTRSLLANLSAFSRAV
                     NVPHLADLTPRVWFAYVQERIKAGIKPSSLNTELKVVQSLIRFASENNIPVCRMMLEV
                     HPLKTGEALPKDLNMGQVKALLNAARNKTEEAWILLMLHCGLRTCEIRALRWEDVDLQ
                     KRVIYIRESKGLQSRAVFLSLPVKEALEGLPQNHGFIFTYRNESLSRRYCQSRLSTLG
                     RQVGFHVTPHQLRHTCATMLLNAGMSILGVQRLLGHKYVETTLRYARLYDVTVVRDFW
                     KVSDVK"
     gene            complement(2494..>3337)
                     /locus_tag="PKI33_06745"
     CDS             complement(2494..>3337)
                     /locus_tag="PKI33_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNM36739.1"
                     /translation="TPFSDWLICQYPRSSARKHYLSDLALFFSWIGKPPSEISPQDVA
                     QYIHHCLEKSLSPLTINRRLSSLRLFYYFLRIIQEEVLDCPVQKRHYLRKPQPLPRDA
                     SEEQITALFSIITDKRDKAIFTLMLECGLRVGEVCALSLDDVLLEDPPRLKVHGKGGR
                     QRIAYLPPPALSVLTDWLTSRPVTKDRAVFISQRVKRLSVNGIQFILKEYFQKAGIQV
                     TCHQFRHAFGRRMVEASMPITSLQKLLGHRSPRTTQRYVNLTNPALQADYDRAIQKAL
                     GSLP"
BASE COUNT          941 a          671 c          794 g          931 t
ORIGIN      
        1 ggattccaca cagttaattt acctcaggtc cagatattac aaccccgccg atgggaggtt
       61 ccagtcacgg gatacgtgga gtggggatta caaccgtccc ttgtcgctga accggtggat
      121 gtacgtcgag gggaatcctg tcaagtatac ggatccaagt ggattctgcc ctgaacctcg
      181 aacagatgaa gacccatgct ggggtcttct acttcaaatt gaatctcgtt atagttttgt
      241 ggatttagag tctgagttga gtaaatttaa tttttggact tacgaagaat tattatatat
      301 acaaaaagac ctcgaaagat ttgaccaagc ttctaaagtt gacttaggca cactttatcc
      361 ctctagcgta aaattaaaga gaattttggc taatgacaat aaggcttgtg cagaaacgaa
      421 tcgctatttt gtaaaaaaca gctccattgt tttttataac agctttgata aattgtgtgg
      481 ggaaggggtt ttggtgcatg aattagggca ttatcttgat ggtataaatc agcgaaagta
      541 tagtaaagaa ttcgaggaat atgttggggc attcactttt ctttggtggt acaaccttgg
      601 acaagagaac ccaccaattt atggctcagg ttatcctcct aatcgtcgcg aagatttcgc
      661 cgaatcacta gaagaatatg ttttacttta cacaaattat tcaggaccag acattggcat
      721 agttcctggc gaaaaaagat ggaaatttat tgagtttgtc ttagacacag gacaattatc
      781 tataaataaa gagcaatacc tctgtcttcc cagctatggt aaattgataa atctcaatta
      841 gagattcaat ggtgagttat gaagattaaa acaattattt tgcgcatatt gctaattcca
      901 cttcctttga ttttggtact aacaggttgc aaagattcta aaggaactgt tttagttggt
      961 acaagagatt gcgagccacc ttgctggtta gatattatgc caggagttac aaaaagtggt
     1021 gatatgatag aaattcttaa gaagaaagaa caggcaaaag aaggattttt aacacttttg
     1081 gatggtggaa ttataaggtg gcaatcactc gatggttaca actcttatgt ttacccgtca
     1141 gagaacgaag ttgttaccag aatcgaggtt gacttgcgat ctaattcaat aaagttggat
     1201 gatttaatta gcatttttgg agagccttta acattagata ttggaaaagt tgaagatgga
     1261 tattttcgtt ctactgtatt ttatccaaat aagggattag cattcgttat tagtggtgat
     1321 gaaattgata ttaccacttc ggatttgaag tttcttttca accccgatat ggcggtttta
     1381 aaaggtattt tttttagtcc aatggaagat atatctacta tgatcaattt actttattcg
     1441 tcaaatgcca aacccagcgt attgttaaat atccaaaatt ggacaggata tgggatttat
     1501 tataaataat attggaactg aaagatgtat ggcaacatcc tacgtataaa ttttcaggaa
     1561 attaccatac atcctaaaac ttacttgctt atcaataccc acactccaac gtcagaaagc
     1621 acatcgtcaa cgactagagt cggtttcttt atttcacatc acttaccttc caaaaatctc
     1681 tcacaactgt gacatcgtac aaccttgcat atcgcaaggt cgtctccaca tatttgtgtc
     1741 ccaacaacct ctgcactccc agaatggaca tccccgcatt gaggagcatc gtcgcgcagg
     1801 tgtgcctgag ttgatgaggt gtgacatgaa acccgacttg cctgccgagg gttgacaacc
     1861 tgctctgaca atagcgacga ctcaaagact cgttacgata ggtgaatata aatccatgat
     1921 tttgcgggag tccctccagt gcttccttta ctggtaggct cagaaaaaca gcgcgacttt
     1981 gtaagccctt agattctcgg atgtagataa cccgcttttg tagatcaaca tcctcccagc
     2041 gcaaggcgcg gatttcacaa gttcgtaacc cacaatggag catgagtaat atccaggctt
     2101 cctcagtttt gttacgagcg gcatttagca aagctttgac ctgacccatg ttcaaatcct
     2161 tgggtagagc ctcccccgtc ttcaagggat gtacctccag catcatcctg caaacaggga
     2221 tgttgttctc acttgcaaat cgaatcaggg actgaacgac tttcaattct gtgttcagcg
     2281 aggaaggttt gatgcctgct tttatcctct cctgaacgta ggcaaaccag accctgggcg
     2341 tgaggtctgc aaggtggggc acattcacag cacgagaaaa tgccgatagg ttggcaagca
     2401 ggctgcgggt atggaagagg cgttctgatt ccctccacga acgggggcga tgattcacat
     2461 atccaaggat gcaagttttc cattctggcg agttcatggc aaactcccca acgccttttg
     2521 gatcgcgcgg tcatagtcag cctggagcgc agggttggta agattgacat agcgctgtgt
     2581 ggtacgcgga ctgcgatgtc cgagaagttt ttgcagggat gtgattggca tggatgcctc
     2641 taccatacgc cgcccgaagg catgacggaa ttgatgacag gtgacttgaa tgcccgcttt
     2701 ttggaaatat tctttcagga tgaactggat accattgacg gagagccgct tgacacgctg
     2761 ggaaataaaa accgcccgat ctttcgtgac cgggcggcta gtgagccaat ctgtgagtac
     2821 ggataaggct ggtggaggga gataggcaat gcgttgcctg cctcccttgc catgaacttt
     2881 tagccgggga gggtcttcga gaagaacatc gtccaaggag agagcgcaga cttcacccac
     2941 ccgcaaaccg cattcgagca tgagggtgaa gatggcttta tcgcgtttgt cggtgatgat
     3001 tgagaacaga gcggtgattt gttcctcgct ggcgtcgcgc ggaaggggtt gaggcttacg
     3061 aagataatga cgtttctgga caggacaatc caaaacctct tcctgaatga tcctgaggaa
     3121 ataataaaac aaccgcagtg aggaaagcct gcggttgatc gtgagaggag ataacgattt
     3181 ttcgagacag tggtggatgt attgggctac atcctgtggg gatatttccg agggtggttt
     3241 tcctatccat gaaaagaaaa gcgccaggtc actgagatag tgtttcctgg cgcttgaacg
     3301 cggatactgg cagatcagcc aatcactgaa tggggtg
//
LOCUS       DAOREF010000267         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18372_length_3336_cov_4.873514, whole genome shotgun sequence.
ACCESSION   DAOREF010000267 DAOREF010000000
VERSION     DAOREF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18372_length_3336_cov_4.873514"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PKI33_06750"
     CDS             complement(<1..1214)
                     /locus_tag="PKI33_06750"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNM36740.1"
                     /translation="MKTIRRLYFYAVAAISIEVVLWGIIGLLRSILGAQDISNNATTL
                     ASALALIFVGVPIFLIHWGWAQNVSSKDSEEHSASIRAVFLYGILLGTLIPVVQNLLA
                     LINRTFLSTVHLSSYRAVVGGYQTWLDNLIAIIINLLIAAYFWNVLRSNWKTITEPES
                     FAEVRRLYRFVWVLYGLLMAVYGVQQAIAYAFSFPTGLLGEVGRETAVNAIALMVIGT
                     PIWYYTWRLLQDALTDTDEKESYLRLGLLYLLTLGGILVFLFSCGDLLYLIFLQVLGD
                     GKSASEFIRELGGALSVTVPFGVVWAYYSKWLNQQFAFDENLPRRAGKHRLYIYILSF
                     VGLTATTGGLISLFGVLIDLTLGNRYLSDSGMREPLAGAFASLVVGLPLWLLNWRVAQ
                     AQALADSDLGDHA"
     gene            complement(1258..1731)
                     /locus_tag="PKI33_06755"
     CDS             complement(1258..1731)
                     /locus_tag="PKI33_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36741.1"
                     /translation="MKQDKFLTGILIGIGALVVIALALFFTRQDKHDYLPEDTPDGVA
                     YNYVLAVLNKDYEKAYGYLADLKNKPTYEDFRQSFLNNMVNPDGTGADIGDATINGDE
                     AVVDVILYYSYSDPFSSRTGSPDRALLVKQDGEWKLSSMPYTFWDWNWYQEPYNP"
     gene            1853..>3336
                     /locus_tag="PKI33_06760"
     CDS             1853..>3336
                     /locus_tag="PKI33_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36742.1"
                     /translation="MSDSSYNFNSAIEDFRSARQRASIQEVIARVMGRSNQLLSYDDV
                     AKKLRLQSRTERGLKNIPLDAIVGSVGRYTDFTRTFLPLQDSDRERWASVKAAMVTGA
                     GLPPIEVYQVGDVYFVLDGNHRVSIARQEGLKSIEAYVTEFRTDVKLTPETKLDEIII
                     KAEYIEFLEKTDLAQARPNVDLSVTIPGQYEKLMEQIDVCKYMDENETLTLQDAAANW
                     YDTLYIPLAEAIRDRELLQWFPGRTITDLYIWISENRAALEKELGWELRSDVAATDLI
                     LKRSVASESGSWRKARTIARYTDHLFEDILIPLSGQTESWDALYQGIVIAQRDQARLH
                     GLHIAKTKEEAEGAEAQEIKERFERVCKDAGVQGSLAIDVGDITQRIIERAVVTDLII
                     VKLLNPPGSGLSVLNSPFRAIIEKSSRPLITVPKGATPFKRAMLAFDGSDLAKEALFV
                     TAYLAEMWKTEVTVFTAREEKSPQGDLKDYARRYMELHEVEAKYV"
BASE COUNT          939 a          855 c          793 g          749 t
ORIGIN      
        1 gcatggtcac ccagatcgct atcagcaagt gcttgagctt gcgccacacg ccagttcagc
       61 agccacaatg gcaagcccac caccagcgac gcgaatgcac ccgccaaagg ctcgcgcatt
      121 ccagagtcgc ttaggtagcg attgcccaag gtcaaatcga ttaaaacgcc aaacagagag
      181 atcaagccgc cggtcgttgc tgtaagcccc acaaacgaaa ggatgtagat gtacaggcga
      241 tgtttccccg cccggcgtgg aagattctca tcgaaagcaa attgttggtt gagccatttg
      301 ctatagtatg cccacaccac accgaacgga acggtgacag agagcgcccc acccaattca
      361 cgaataaatt cactggcaga cttgccatcc ccgagcactt gtaaaaagat caggtataac
      421 aagtcgccgc atgaaaatag aaagacgaga atcccaccga gggtaagtaa gtagagcagc
      481 ccaaggcgta gatacgattc cttttcgtca gtatccgtta gcgcgtcctg taagagccgc
      541 caggtgtaat accaaatcgg cgtaccgatc accatcaaag caatcgcatt gaccgcggtt
      601 tctcgtccca cttctcccag caaaccggtt gggaacgaga aggcatatgc aatggcttgt
      661 tgcacgccgt agactgccat caacaaccca tacagcaccc acacaaaacg atacaaacgg
      721 cgtacttccg cgaagctttc gggttcggtt atggtcttcc aattggaacg tagcacattc
      781 cagaaatagg cggcgatcaa caagttgata atgatcgcga tgagattatc caaccaagtt
      841 tgatacccgc cgaccacagc ccggtatgaa gagagatgca ccgtggaaag aaaagtgcgg
      901 ttgataagcg ctaacaagtt ttgcacaacc ggaatcagcg tgcccagcaa aattccatac
      961 agaaaaaccg cgcgaatgct ggctgaatgt tcctcggagt cttttgaaga aacattctgc
     1021 gcccaacccc agtgaatcag gaagatgggc acgcccacaa agatcagcgc caatgccgaa
     1081 gcaagtgtgg tggcattgtt actgatatct tgagcgccaa ggatggaacg cagcaaccca
     1141 ataatgcccc ataacaccac ttcaatgctg atggcagcaa ccgcataaaa ataaagacga
     1201 cgaattgttt tcatagttga tcctcagtca ttacgagttt tgcaatgaca tgaaaaatta
     1261 cgggttatac ggttcttgat accagttcca atcccagaat gtgtatggca tggagcttag
     1321 tttccattcg ccatcttgct tcaccaacaa ggcacgatct gggctgcctg tgcggctgga
     1381 gaatgggtca ctgtaagaat agtagagaat gacatccacc acggcttcat cgccattgat
     1441 ggttgcatcg ccaatgtcgg ctccggttcc atcggggttg accatgttgt tcaagaacga
     1501 ttggcggaaa tcttcataag taggcttgtt ctttaagtct gcaaggtagc cataggcttt
     1561 ttcataatct ttgttgagaa ctgccagcac atagttgtaa gcaactccgt ccggcgtatc
     1621 ctctggcaaa tagtcgtgct tgtcttgccg ggtgaaaaag agcgcaagcg ctattacgac
     1681 caaggcacca ataccaataa gaatgccggt gagaaattta tcttgtttca tgagaatacc
     1741 tccataattt aactgtacac taaattgacg aaaatgacat ttcaaaagtt tcatacttga
     1801 aggggaactt cattcacttt atactcattc atgaggaacg ttttacggaa gcatgtctga
     1861 ttcgtcatac aactttaatt ctgccatcga agattttcgc agtgcccgtc aacgcgcttc
     1921 cattcaggaa gtgatcgcgc gggttatggg gcgttccaat cagttgttat cgtatgacga
     1981 tgtcgcaaaa aaattacgtc tgcaaagccg caccgagcgt ggactgaaga acattccgct
     2041 cgatgcaatt gttggcagtg tggggcgtta taccgacttc acccgcacct tcttaccgtt
     2101 gcaagatagc gaccgcgaac gttgggcaag cgtcaaagcc gccatggtca ccggagcggg
     2161 gcttccaccc attgaggtgt atcaggttgg cgatgtgtat ttcgtgctcg atggcaatca
     2221 tcgcgtctca attgcaaggc aggaaggctt gaaatccatc gaagcctatg tcaccgagtt
     2281 tagaaccgac gtcaaactca cgccagaaac caaattagac gaaatcatca tcaaagccga
     2341 atacatcgaa tttctcgaaa agaccgatct tgctcaagct cgccccaatg tggacttaag
     2401 cgtcaccatc cctgggcaat acgaaaagtt gatggaacaa attgacgttt gcaaatacat
     2461 ggatgagaac gaaaccctca cgcttcaaga cgccgccgcc aactggtacg acaccttata
     2521 tattcccctc gctgaagcca ttcgcgaccg tgaactactg caatggtttc ctggtcgcac
     2581 catcaccgac ttatacattt ggatatcaga gaaccgtgcc gcccttgaga aagagcttgg
     2641 gtgggagtta aggtctgatg tggcagctac agacctaatc ttgaaaagaa gcgtggcatc
     2701 tgaatcgggc agttggcgca aagcccgcac catcgcgcgt tacaccgatc atctctttga
     2761 agatatcctc atccccctca gcggacaaac cgaaagctgg gatgcgcttt atcagggtat
     2821 tgtgattgca caacgtgacc aagctaggct gcacggttta cacattgcga aaacaaaaga
     2881 agaagccgaa ggggctgaag cgcaagaaat caaagaacgc tttgaacgag tctgcaaaga
     2941 tgcaggtgta caaggcagcc tcgccattga cgtgggcgac attacgcaac gcatcatcga
     3001 acgcgccgtt gtcacagacc tgatcattgt caaattgctc aacccgccag ggtcggggtt
     3061 atcggttttg aactcgccct tccgtgccat tattgaaaaa tcatcccgcc cgcttatcac
     3121 cgtgcccaaa ggcgccacac ctttcaaacg agccatgctt gcttttgatg ggtctgacct
     3181 tgccaaagaa gcgctcttcg tcactgccta tcttgccgaa atgtggaaaa ccgaagtgac
     3241 cgtgttcact gcccgcgaag aaaaaagccc gcaaggtgat ttgaaagatt atgcccgccg
     3301 gtatatggaa ttgcatgaag tggaagccaa gtatgt
//
LOCUS       DAOREF010000268         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18507_length_3323_cov_3.726132, whole genome shotgun sequence.
ACCESSION   DAOREF010000268 DAOREF010000000
VERSION     DAOREF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18507_length_3323_cov_3.726132"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PKI33_06765"
     CDS             <1..1371
                     /locus_tag="PKI33_06765"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNM36743.1"
                     /translation="SIILDGVDITAAVCPPTVSAVGNPADAANAGRQVVFNIGTIPAP
                     ATDTTLVIRYDVIVLDINTNQQGVALNNSATVTWSGGTLSSSAPNVNIVEPDMSIDKS
                     ATPNQGVPLGTPIQFTLVIDHTTPGSQTDAFDVVVTDILPPGLEYVQCSVQYTAGLAP
                     DTPASDYCNLGTVTTDLTFEWAVFPLGDTSTITFTARLISSPVMNSASVAWTSLPIDP
                     QVNGLPVQISAHNTQSTERWYDPLDDVNVYSITDTASINAPATSSSGNSGDEDASLPS
                     ILPKTGFAPNHVTLLPEQPTEQAYRATEVWLEVPRLGVTMPIVGVPLVGEDWNVSWLW
                     NEAGWLEGTAFPGWQGNSVLTSHVTLANGVEGPFAKLNNLKYGDQIILHAYGSLYIYE
                     VRQNHTVSPYNTTVLQHEEASWITLLTCANYNENTNTYSNRIAVRAILIRIMEDKVNS
                     VITNIR"
     gene            1433..1918
                     /locus_tag="PKI33_06770"
     CDS             1433..1918
                     /locus_tag="PKI33_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM36744.1"
                     /translation="MNSKNDSDNAGYNEYMADQQPLKIFISYASGDRAKAQQLHAYLV
                     QHSTEPWLDTENLLPGQDWKMEISKALDETNLILLCLSKTSVTREGYVQKEMRLALDR
                     ALEMPQGEIFLIPARFEEVDLPYSLRDYQWVDLYTESGKSKLLKSLALRAQKVGAKLP
                     I"
     gene            complement(1978..3297)
                     /locus_tag="PKI33_06775"
     CDS             complement(1978..3297)
                     /locus_tag="PKI33_06775"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HNM36745.1"
                     /translation="MANINLPGPKAKAMIERDHKVISPSYPRGYPFAMDHGKGTEVWD
                     VDGNRFLDFMGGIAVVATGHSHPQVVKAIQEQAEKFIHISSDFYHENWIKLGEKLNEI
                     APFEEDALSFMTNSGTEAVEAAIKLARYHTKRSNFIGFTGAFHGRTMGAVTFTASKSK
                     YHSGFYPLMNGVTHAPYPNPYRPVLERRKGEDYGEAVVRYIEEEIIAQILPAKEVAGV
                     LVETIQGEGGYIVPPDGFYPALRKFCDKHGILLIVDEVQSGMGRTGKWWAIEHFGVEP
                     DIVTSAKGIASGMPLGACIARKSVMDWEIGTHGNTYGGNPISCAASLATIDLIQKQYL
                     QNAKEVGEYAMDALMEIQSRHPSIGEVRGKGLMIGVEFVKDRETKEPAKHLTERVVDI
                     AYERGLLMLSCGQSVIRIAPPLSISKSEVDEGLRMFEEAITQAEKEK"
BASE COUNT          901 a          763 c          724 g          935 t
ORIGIN      
        1 tcgattattc tggatggcgt tgatatcact gcggctgtgt gcccgccgac ggtttctgct
       61 gtgggcaacc cagccgatgc tgcgaatgct ggtagacaag tagttttcaa tatcggcact
      121 atccctgccc cagctactga tactacgctt gtgatccgct atgacgtgat cgtgctggac
      181 atcaacacca accaacaagg tgttgcgctg aacaacagtg ccacagtgac ttggtcaggt
      241 ggaacacttt catcgagtgc tccgaatgtg aacattgtcg aaccagatat gtctattgac
      301 aagagcgcaa cccccaatca aggtgttccg ttaggcacgc ccatccaatt caccttggtg
      361 atagatcaca ccaccccggg aagccagaca gatgccttcg atgttgtcgt cactgatatt
      421 ctgccgcctg gacttgaata tgttcaatgt tcggttcagt acacagctgg tttggctcct
      481 gatacgccag ctagtgatta ctgtaactta ggtacagtta caaccgatct tacctttgaa
      541 tgggcggtct ttccgcttgg agatacttcc actattacat ttactgctcg gctaatcagc
      601 tcgccggtga tgaatagtgc atctgtagcg tggacctcgc ttccaataga tccacaggtt
      661 aatggattgc ctgtccaaat ttctgcccat aatactcaat cgacagaacg ttggtacgat
      721 ccgcttgatg atgtgaacgt atatagcatt actgatacag cgtcaatcaa cgcacctgcc
      781 acaagtagca gcggaaatag tggtgatgaa gatgcatctt taccaagtat cttgccgaag
      841 acaggttttg cgcccaatca tgtaacgctt cttccagagc aacctacgga acaggcatat
      901 cgtgccaccg aagtttggtt ggaggtgcca cgcttgggtg tgacaatgcc tattgtaggt
      961 gttccgcttg ttggggaaga ttggaacgtc tcttggttgt ggaatgaagc tggttggctt
     1021 gaaggtactg ctttccccgg ttggcaaggg aatagtgtcc ttactagtca tgttactttg
     1081 gcaaatggtg tggaaggccc gttcgcaaaa ctgaataacc tgaagtatgg agatcagatt
     1141 attctccatg cttacggtag tctgtacatc tatgaagtac gccaaaacca cactgtcagc
     1201 ccatataaca ccactgtcct tcagcacgaa gaggcttcct ggattacatt gttaacatgt
     1261 gcaaactaca atgaaaatac caacacctat tcaaaccgaa ttgccgttcg tgcaatctta
     1321 atccgtataa tggaagataa agtcaatagt gtaataacta atattcgcta atacttaaac
     1381 cttttgatag atcgtaggaa taggaaaagg cagcgtcgaa agatactgcc ttttgaattc
     1441 taaaaacgac tctgataatg ctgggtataa tgaatatatg gcagatcaac aacctctaaa
     1501 aatatttatc agctatgcat ctggtgatcg ggcaaaggcg cagcaattac atgcttatct
     1561 tgttcagcat agcacagaac catggctcga tacagaaaat cttttaccag gacaggattg
     1621 gaaaatggaa atttccaagg cattggacga gactaatctc atcctacttt gtctctcaaa
     1681 aacttctgtt actcgtgagg gatatgtcca aaaagagatg cgcctcgcac tagatcgagc
     1741 actggagatg cctcaaggag agatttttct catcccagcc cgttttgaag aagttgatct
     1801 tccatacagc ctgcgcgatt atcaatgggt agatttgtac acagaaagcg gtaaatcaaa
     1861 attattgaag agcttggcat tgcgtgcaca aaaggttgga gctaaacttc ctatatagtt
     1921 ttgccataaa aaagagaccc tcaaattatt tgagggtctc tttttttatg gcaatggcta
     1981 tttttctttt tctgcttgtg taattgcttc ttcaaacatt cgtaagcctt catctacttc
     2041 gcttttgctt atagaaagtg gaggtgcaat acgaataacg ctttgtccac aagatagcat
     2101 caacaacccg cgttcgtaag cgatgtcaac aaccctttct gttaagtgtt ttgccggctc
     2161 tttggtttca cgatctttta cgaattcgac cccgatcatt aaacccttcc cacgaacttc
     2221 gccgatgctg gggtgtctag attggatttc cattaaggca tccatggcat attcaccaac
     2281 ttctttggcg ttctgcaaat actgtttttg gatcaagtca attgtcgcga gagaggcggc
     2341 acaagaaata gggttgccac cataggtatt cccgtgtgta ccgatttccc aatccattac
     2401 agattttcgt gcaatacatg caccaagcgg catgcctgaa gcaatccctt ttgctgaagt
     2461 aacaatatct ggctccacac caaaatgctc aattgcccac catttaccag tgcggcccat
     2521 gccagattgc acttcatcta caattagcaa aataccgtgt ttatcgcaga attttcgtag
     2581 tgctgggtaa aaaccatctg gcggaacaat ataaccgcct tcgccttgaa tcgtctcaac
     2641 taatacacca gcaacttctt tggctggcaa tatttgcgcg atgatttctt cttcaatgta
     2701 acgaaccact gcctcaccat aatcttcacc tttgcggcgc tctaagacgg ggcgataagg
     2761 attagggtag ggagcatgtg ttacgccatt catgagcgga taaaatccac tatggtattt
     2821 tgatttgcta gcagtaaaag tgacagcacc catggttcga ccatgaaatg cgccggtaaa
     2881 gccaatgaag tttgaccgtt tcgtatgata acgagcaagt ttgattgctg cttctactgc
     2941 ttcagtaccg gagttggtca taaatgagag cgcatcttct tcaaaaggcg cgatttcatt
     3001 tagtttttca ccaagtttga tccagttctc gtgataaaag tcagaagaga tgtgaatgaa
     3061 cttttctgcc tgttcctgaa tggctttgac aacctgtgga tgagaatgac ccgttgcgac
     3121 cacagcaata ccacccataa aatctaggaa gcgattgcca tctacatccc aaacttcggt
     3181 gcctttgcca tgatccatcg cgaacggata tccacgtgga taggaaggag agatcacttt
     3241 gtgatcccgt tcgatcattg cctttgcctt tggaccggga agatttatat ttgccataac
     3301 tactccttgg aatgaattga aaa
//
LOCUS       DAOREF010000269         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18541_length_3320_cov_3.846554, whole genome shotgun sequence.
ACCESSION   DAOREF010000269 DAOREF010000000
VERSION     DAOREF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18541_length_3320_cov_3.846554"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1988)
                     /locus_tag="PKI33_06780"
     CDS             complement(150..1988)
                     /locus_tag="PKI33_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36746.1"
                     /translation="MNTHPTTSQYFDFRDALHENRLLGLWRMMTDFRLSYIAATISLA
                     FSALAKTSVYLLLRFFADDVLVQGKMIGSTMTQTFLWIGLGFIGLAFIEGGGAFLSGR
                     LAAYTAEGITRRLRDFLFDHIQRLSFSYHSATPTGDLIERVTSDVDALRRFFSEQAIG
                     VGRIVLLFAINWAAILYINVKLGLVSVVTIPLILWVSLWFFKKVTKAYEDYQAQEAVL
                     STTLQENLTGVRVVKAFARQDYEMEKFEKDNWLKYIKGKFLLLMHSLFWPLSDIVLGG
                     QMLFGFIFAGFMAINKEITVGDYVAYVGLLVWLIFPIRNLGRVIVQTSTGMVSYQRLM
                     DITKQARENLNGGKVQPTGPVRGEIEFKDVSFMYSDGTHDVIQNVSFHIKPGESIALL
                     GSTGSGKTSMVNLLPRFHDYTGGQILLDGVDLKDYPRKYLREQIGIVQQEPFLFSRSI
                     RENILYGVGRSVTQAEIEDAAKAAAVHDVIIGFPDGYNTIVGEKGVTLSGGQKQRVTI
                     ARTILKNPKILILDDSTSAVDTETEASIRQALNDLMEHRTTFIIAHRIQSVMKADQIL
                     VLDRGQVVQHGTHQELVGQFGGMYRKVFDIQTRIDEELENEIARAN"
     gene            2320..2691
                     /locus_tag="PKI33_06785"
     CDS             2320..2691
                     /locus_tag="PKI33_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNM36747.1"
                     /translation="MTHVITSLCLRDNGCSSVCPVESINPGNPAQGWYTYYIDPSSCI
                     DCGACVPECPFHAIYPADEIPQAYKAKGGELINMAGLNGHYEGMSHTGEVIRLDSTRA
                     LTAGEVMDLTPAIALNEKFYK"
     gene            complement(2695..>3320)
                     /locus_tag="PKI33_06790"
     CDS             complement(2695..>3320)
                     /locus_tag="PKI33_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36748.1"
                     /translation="LRNGFNLPALPSQSAATTESAAPLSTSQPVLPTDTPVPSPTFTA
                     TETIVPPTATIEPSPTIVLTPTVKYPEGANYTLFYNETSLFIINRSYERRSISGFKFQ
                     NLNADGTLGEEVFDGYRWETRNVDYLPRNFCANISIFGDEDPPYLNPVDCQQYGSIQT
                     VQTRFDRPEKILFWVPKGGTTQFVVFWLNEEVARCEITAGICEVYIP"
BASE COUNT          906 a          765 c          807 g          842 t
ORIGIN      
        1 tacccatcgc caatgcggct taatgatccc cataatccgc ttgaagaccg ggaaggttat
       61 ctgggatgta tgttcttctt cttcaagttc caataattca tctgacattt tttattctcc
      121 gtatgggcga gaatctcgct catacatcat taattcgctc tcgcaatttc attctctaat
      181 tcttcgtcaa ttcttgtttg aatatcgaag accttgcgat acataccgcc gaactgacca
      241 acaagctcct ggtgtgttcc atgctgaaca acctgacctc tatccagcac caggatttga
      301 tctgctttca tcacggattg gatgcggtgc gcaatgatga aggtcgtgcg gtgctccatt
      361 aggtcgttca atgcctggcg aatggacgct tccgtttccg tgtccactgc cgaggttgag
      421 tcgtcaagga taagaatctt cggatttttc aagatcgtcc gtgcaatggt cacacgttgt
      481 ttctgaccgc cagatagagt tacgcctttt tcaccgacga tggtgttgta gccgtcgggg
      541 aatccaataa taacgtcatg tacagcagca gcctttgcag catcttcgat ctctgcctgt
      601 gtcacactgc gacccacacc gtacaaaatg ttctctcgga tcgaacgact gaacaagaag
      661 ggctcttgtt gcacaatacc gatctgctcc cgcaaatatt tacgtggata atctttcagg
      721 tccacaccat cgagcagaat ttgtccgccg gtgtaatcat gaaagcgtgg cagtaaattc
      781 accatcgatg tcttacctga acctgttgac ccaagcaaag cgatcgattc acccggcttg
      841 atatggaatg aaacattctg gatgacatca tgcgttccat cagaatacat aaacgataca
      901 tctttgaatt cgatctcgcc tcttaccgga cccgtcggct ggaccttgcc gccgttgagg
      961 ttttcccgcg cctgtttggt aatgtccatc agacgctggt acgaaaccat accggttgat
     1021 gtttgtacga tcacacgccc aaggttgcgg atggggaaga tcaaccaaac cagcaagcct
     1081 acgtatgcta cataatcgcc aacggtgatc tctttgttga tcgccataaa gccagcaaaa
     1141 ataaacccaa agagcatctg tccaccaagg acaatatccg aaagaggcca gaaaagggag
     1201 tgcatcagca aaaggaactt gcctttaatg tatttcagcc agttatcctt ttcaaacttc
     1261 tccatttcgt agtcctgccg ggcaaaggct ttcaccacac gaaccccggt caggttctct
     1321 tgcaacgtag tagagaggac cgcctcttgc gcctgataat cttcataggc ttttgttacc
     1381 tttttgaaga accacaagga aacccacaaa ataagcggaa tggtcactac agaaaccagc
     1441 cccaacttca cgttaatgta caagatcgca gcccaattga tcgcaaatag cagcacgatt
     1501 ctgccgacgc cgatggcttg ctccgaaaag aagcgccgta aggcatccac atctgaagtg
     1561 acgcgttcga tcaagtctcc ggtgggtgtg gcgctgtggt acgagaagct caagcgttga
     1621 atatggtcga agagaaagtc tcgtaaacgg cgggtgatgc cttcggctgt gtaagcggct
     1681 aaacgtccgg aaagaaacgc gccgccgcct tcaataaagg cgagtccaat aaatcccagc
     1741 ccaatccata ggaaggtttg ggtcatggta gagccgatca tcttcccctg tacaagaaca
     1801 tcatctgcaa aaaagcgcag gaggaggtat acagatgtct ttgccagggc agagaaggct
     1861 agtgagatcg ttgcagcaat ataggacaga cgaaaatctg tcatcatacg ccacagaccg
     1921 agcaggcggt tttcatggag cgcgtctcgg aagtcaaagt attgggaggt ggtagggtgg
     1981 gtattcatag tgtttctccg aacgtaacga aaaacgatta aataaaaagg ctgcggcgcg
     2041 tgcgccacag cccaaaaacg acaaggaact gaaagagacc tagggacggt caacaggcgc
     2101 actccaagaa ggcaatttgc caactgcatc gagagtttgg atgaagattg tgttgaacat
     2161 caagagtgcg ctcctttctt tgtggatttg aattacatta tgtagtttac acctctcgtt
     2221 tgaaaaatgt caaggttaag ttacggatta gaaattaatt tttccgataa cttttataac
     2281 tagtaagcga ctggatgaat atatcaaaag gagcctaaaa tgacccatgt aattacaagc
     2341 ctatgtttgc gtgacaatgg atgttcatct gtttgcccag tggagtccat caacccaggg
     2401 aatcccgctc aaggctggta cacctactat attgacccgt ccagttgtat tgactgtggt
     2461 gcttgtgtgc cagaatgccc gttccatgca atttacccgg cggatgaaat tcctcaggca
     2521 tataaagcaa aaggcgggga gcttatcaat atggcaggac tcaatggtca ttacgagggg
     2581 atgagtcata caggcgaagt cattcgttta gactctaccc gtgctttaac agcaggtgaa
     2641 gtcatggact tgacacctgc aatcgcattg aacgagaaat tttataagta aagatcaggg
     2701 aatatagact tcacaaatgc ctgctgtgat ttcgcagcgg gcaacttctt cattaagcca
     2761 aaagaccaca aattgcgttg tgccgccttt aggaacccaa aagaggattt tttctgggcg
     2821 gtcgaagcgg gtttgtacgg tttgaataga accgtattgc tggcaatcta cagggttgag
     2881 ataaggtgga tcttcatcgc caaaaatgct gatatttgcg cagaagttac gtggtagata
     2941 atctacatta cgcgtctccc aacgatagcc atcaaaaact tcttcgccta aggttccatc
     3001 ggcattgagg ttttggaact tgaacccaga aatactgcgc cgttcatacg aacggttgat
     3061 aataaacaaa ctggtctcgt tataaaacaa ggtgtagttt gcgccttctg ggtacttcac
     3121 tgtaggcgtt agaacaattg ttggcgatgg ttcgatcgta gcggtgggag gaacaatcgt
     3181 ttctgttgcg gtaaaggtag gggaggggac gggggtatca gttggcagaa caggttgaga
     3241 cgtagaaaga ggcgcggcgg attctgttgt agctgccgat tgagaaggca acgctggcag
     3301 gttgaatccg tttcgaagga
//
LOCUS       DAOREF010000270         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18574_length_3315_cov_4.358282, whole genome shotgun sequence.
ACCESSION   DAOREF010000270 DAOREF010000000
VERSION     DAOREF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18574_length_3315_cov_4.358282"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..907
                     /locus_tag="PKI33_06795"
     CDS             149..907
                     /locus_tag="PKI33_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM36749.1"
                     /translation="MKNKKITLFVVLLIMGILLVACGGGTPPAEETQIVDATQAPVEA
                     TATIQPVSTVDLKLDPANLASDNAKAAVPYLYEGLVRLQDGAVVGALASTFTVSEDGL
                     DYIFDLRTGVTFHDGTLLNADTVVLNFNRWFDPADVNRGSGEYAAWVANFGGFKGELT
                     EDGKPKSPVDGIEKQDEFTVIIHLNTPDPDLLTKLTDPAFSIMSASSFAGNDGGTGPY
                     KAASNDGTTITLEPFAGYWDPAAIPSANVEIPAP"
     gene            complement(959..2101)
                     /locus_tag="PKI33_06800"
     CDS             complement(959..2101)
                     /locus_tag="PKI33_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNM36750.1"
                     /translation="MTLFLQKHRHIFTLAGAILLALAVSACGENIDLSGAAGLGNSTD
                     VAATDVVATEETAVVAEAPTATPLPAATGHIIFASNRDGQTDLYMTTPDGAELTRLTT
                     GAAVDNGSPIRLSPDGTKIAFSATLGGNTDVYVFDLATNFTTRITDAQEKDSSPSWSP
                     NSQQLAFESFRDGNIEIYIANADGSNPIRLTNDPAGDSNPIWSPVSNEILFTSNRFGN
                     SDLMLLNLNGTVSTLTTSRAPDNAAAWSPDGNFIAYQSFSGDISNICIIGRDGLNQVC
                     PSIPAEYGTPVWSPNGTQIAASTLSNGVYSISLFNSRDGSSQQISQPGIEPRGTPAWS
                     PDGLRLVLQTQADGDMELFSVFVQTNEFTRITTIAGYDGEPTWIVQ"
     gene            complement(2242..3156)
                     /locus_tag="PKI33_06805"
     CDS             complement(2242..3156)
                     /locus_tag="PKI33_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36751.1"
                     /translation="MSQSQRTVISSTSPLKKIMHAVRAIEEGDYDPILLSEFMTETGD
                     LGKLAQMLDSMANTILKRNNQLTLLSKVIPIGVSLSAEKDFNRLLESLVEEARTFTNA
                     DAGSLYLVEDDKLRFVISRNTSLKMHMGGTSGTPITFVPVRMYNEDGSENRANIVSYA
                     ALTRQRINIADAYEAEGFDFSGTKAFDENTRYRSKSFLTIPLETKEGKVMGVLQLINA
                     TDPKTKEIVPFQDDTVLEALILLATAALEGYIREAALRSEIAKLRIEIDQSRRAKQVE
                     EITDTAFFRELKTKATEMRSKTKKEDEE"
BASE COUNT          810 a          732 c          869 g          904 t
ORIGIN      
        1 tgccccacat ttcggcgtac atttcagagg ggagcagata acgcaagcgg ctttcaataa
       61 tggcaagcgg tgactctgga agcatgattt ccattatact tgaattgaag atgtttactt
      121 tcaattctat ctaaaggcag agagaaaaat gaaaaacaaa aaaataacct tgtttgtagt
      181 tctcttaata atggggattt tacttgtagc ctgcggcggc ggaacgcctc cagcagaaga
      241 gacgcaaatt gtggatgcga cacaggcacc tgtcgaggca accgctacta ttcaacccgt
      301 ctctaccgtg gacctgaagc ttgatcctgc taacttagct tcagataatg ccaaagccgc
      361 tgtgccctac ctgtatgagg gtctggttcg tttgcaagat ggtgctgtgg ttggagcatt
      421 ggcatccacc tttactgttt cagaagatgg attggactat atattcgatc tccgaacggg
      481 tgttactttc catgatggga cattgcttaa cgccgatact gtagtgttga acttcaatcg
      541 ctggtttgac cccgccgatg ttaaccgagg ctcaggtgaa tatgcagctt gggtggcaaa
      601 ctttggtggc ttcaaaggcg agttgacaga agatgggaaa ccgaaatctc cagttgacgg
      661 aatcgagaag caggacgaat ttacagtgat tattcacttg aatacaccag accctgactt
      721 attgaccaag ttaaccgacc cagctttttc gatcatgagt gcttcgagtt ttgccggaaa
      781 tgatggtggg actggacctt ataaagcggc atctaatgat ggcactacca ttacattaga
      841 accatttgct ggttattggg atccagccgc aatcccaagc gcgaatgtag aaatccctgc
      901 tccttaattt tgataataaa agaccccgaa cattgcgttc ggggtctttt attattggtt
      961 attgtacaat ccaggttggt tcgccatcat acccggctat agtggtaatc cttgtaaatt
     1021 cattggtttg aacaaataca gagaagagtt ccatgtctcc atctgcttgg gtttgtagga
     1081 ctaatcgaag cccatcaggt gaccaggctg gtgtgccgcg cggctcaatt cccggctggc
     1141 ttatttgttg tgagctacca tctcggctat tgaaaaggga aatgctgtaa accccatttg
     1201 agagagtgct ggcggcgatt tgagtgccat taggagacca gactggtgtt ccatattccg
     1261 caggaatgga tgggcaaact tggttaagtc catcgcgccc gatgatacag atattcgaga
     1321 tatcgccgct aaaagattgg taggcgataa agtttccgtc tggcgaccaa gcggcggcgt
     1381 tatcaggagc gcggctggtt gtcagagtag agaccgttcc attgaggttg agcaacatca
     1441 agtctgaatt gccgaagcgg ttgcttgtaa aaaggatttc attcgagacc ggtgaccaga
     1501 ttggattgct gtccccggcg gggtcgttgg tcaagcgaat gggattggaa ccatcggcat
     1561 tggcaatata aatttcaatg ttgccatcac gaaaggattc aaaagctagt tgttgactgt
     1621 tgggagacca ggaaggggag gagtcttttt cttgagcgtc tgtgatgcgg gttgtgaaat
     1681 tggtggcgag gtcaaacacg tagacatcgg tgttgccgcc aagcgtggca gaaaaggcaa
     1741 tcttggttcc atctggggag aggcgaatgg ggctgccgtt atccacggcg gcgccggtag
     1801 tgaggcgggt tagctctgcg ccatccgggg tggtcatgta taaatcggtt tgaccatccc
     1861 gattggaagc aaagataatg tgaccggtgg cggcggggag cggagtagct gtgggcgctt
     1921 ctgcaacgac ggcagtttct tcggtggcga ctacatcagt agccgctaca tcggtagagt
     1981 taccaagacc tgctgcacca gaaagatcaa tgttttcgcc acaggcgctt acggcgagtg
     2041 cgagcaaaat agcgccagct agggtaaaaa tgtgtcgatg tttttgaagg aaaagggtca
     2101 tggatcttct acccaatagg gctactttca ggcggattag gactttttta ctatcttttt
     2161 gatttccgta ttgttagtgt tcatttatcc cccaaaagtg tccccagaac aaccttaaaa
     2221 gttgacaatt ttgcaaaccc cttattcttc gtcttccttt ttcgtctttg agcgcatttc
     2281 agtggctttg gtcttgagtt cgcggaagaa agccgtatcg gtgatctctt ccacttgttt
     2341 ggcgcggcgt gactggtcga tttcaatgcg cagtttggca atttcgcttc tgagagccgc
     2401 ctcacggata tagccttcta gggcggcggt ggcaagcaaa atcagcgctt ccaaaacggt
     2461 gtcgtcttgg aaaggaacga tctctttggt ctttgggtca gtggcgttga tcaattgcaa
     2521 cacgcccatc accttgcctt ctttggtttc gagcggaatg gtcaggaatg atttggaacg
     2581 ataacgggtg ttttcgtcaa aggcttttgt accggagaag tcgaaacctt cggcttcgta
     2641 ggcatcggca atgttgatgc gctggcgggt caatgccgca tacgaaacaa tgttggcgcg
     2701 gttctcactg ccgtcttcgt tgtacatgcg taccggcaca aaggtaatcg gcgtgccgct
     2761 cgtcccaccc atgtgcattt ttaaagaagt attgcgcgag ataacgaagc gcagtttgtc
     2821 atcttcaaca agatataaag aaccggcatc ggcatttgtg aaggttcggg cttcttctac
     2881 taaagactct agcaaacggt tgaaatcttt ttcagcagaa agtgaaaccc cgatcggaat
     2941 gactttgctt aacaaggtca attgattgtt gcgcttcaaa atggtgttcg ccatcgaatc
     3001 cagcatttgc gccagtttcc ccagatcccc agtctcagtc atgaattcgc tcaagagaat
     3061 tgggtcataa tcaccctctt caatggcgcg aacggcatgc atgatcttct taaggggaga
     3121 tgttgaacta ataactgtcc gctgcgactg tgacatgttt ttcagtatac cccaagattc
     3181 tgtgcatgag gatgcgatat actaccgcca tgcctaaacg cattggtctc tttggcggga
     3241 ctttcgatcc ccctcatctc ggtcatcttg tccttgcagc agaagcggtc tctcaattta
     3301 agttagatac atttc
//
LOCUS       DAOREF010000271         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18652_length_3307_cov_3.198339, whole genome shotgun sequence.
ACCESSION   DAOREF010000271 DAOREF010000000
VERSION     DAOREF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18652_length_3307_cov_3.198339"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKI33_06810"
     CDS             <1..346
                     /locus_tag="PKI33_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36752.1"
                     /translation="STFPVEGATGWADTTMMHVDAPHPNCAYKWLEWSLNPKLQGDLA
                     AWFGSVPSVPAACTGNELLTDAGCSANGFDQFDNIWFWRTPTAECSTGAGNCVPYSKW
                     VEAYVGIISSGQ"
     gene            434..1471
                     /locus_tag="PKI33_06815"
     CDS             434..1471
                     /locus_tag="PKI33_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36753.1"
                     /translation="MDTPAIRFESVSRHFGDVKAVDQVEMEIRDGEFFSMLGPSGSGK
                     TTCLRMIAGFDRPTSGHIYLYGQDVSNLPPYERDVNTVFQDYALFPHMSVGDNIAYGL
                     MIKKVEKVERMKRVEEMLDLVQLSGFAGRKPSQLSGGQRQRVALARALINHPKVLLLD
                     EPLGALDLKLRQQMQVELKAIQKRVGITFIFVTHDQEEALTMSDRIAVFSQGKIEQIG
                     TPSEVYEHPASQFVAGFVGTSNLISGDVAERITGHAQTFSIRPEKIHIGSQQEYISSD
                     MYAVDGTIRDVIYLGLYTRYLVEITQGIDLVVISQNLKTTSADVFSSKGSAVRLSWQQ
                     DHISILGGKGA"
     gene            1464..2369
                     /locus_tag="PKI33_06820"
     CDS             1464..2369
                     /locus_tag="PKI33_06820"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36754.1"
                     /translation="MLNRISTYLYRHPRLVLGLLLGPPMLYMGVVYLGSLFSLLINSF
                     FYLEEFTGLVVREFTLQTYAKLLDPTNLGIFFRTATMALLVTIADAIIAFPLAYYIAR
                     FAPKKLKTYLYIAVTIPLWSSYLVRVYAWKLILAKEGIVSWFFNLFGLQFILDGILSI
                     PGIGGSSLSLSPIGMFIAFCYIWLPYMIVPIQTALERVPKSLLEASSDLGANPSQTFR
                     QVLLPLSFPGVVAGSIFTFSLTLGDFIIPQVLGNSRFFIGQAVYSYQGTAGNIPLAAA
                     FTMGPIVIMVVYLLIARKLGAFDAL"
     gene            2359..3174
                     /locus_tag="PKI33_06825"
     CDS             2359..3174
                     /locus_tag="PKI33_06825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36755.1"
                     /translation="MRSKSSDSASPALVLAAIGGLLFLHAPIWVIFLYSFTTDEATYT
                     FPLPGLTTKWFGAALQRVDLWNALTLSLQVAVVATLVALVLGTLAAAAVYRSDFFGKE
                     TISLLLILPIALPGIVTGIALRSAIGTLNIPFSYWTIVIGHATFCVVTVYNNVLARFR
                     RMQGSQVEASMDLGANSFQTFRYVVLPNIATALVAGGMLAFALSFDEVIVTTFTAGQQ
                     ATLPIWIFSQLVRPRDRPVTNVVATIVVLITFLPIFFAQRISASSADTEGDSK"
BASE COUNT          727 a          838 c          737 g         1005 t
ORIGIN      
        1 ttccaccttc cctgttgaag gcgccaccgg ctgggcagac accaccatga tgcacgtgga
       61 tgctcctcac cccaactgcg catataagtg gcttgaatgg tccttgaacc ccaaattgca
      121 gggcgacctt gcggcttggt ttggttccgt gccttctgtg cctgccgctt gtacgggcaa
      181 cgaactgctc accgatgctg gctgttcagc caatggtttc gatcagttcg ataacatttg
      241 gttctggcgc acgccgaccg ccgaatgctc caccggtgct ggcaattgtg taccttacag
      301 caaatgggtt gaagcctacg taggcatcat cagcagcgga caataatcct gctgccagaa
      361 ttctagtaac gtgatgtaaa atattcaggc ggggcaatgg ctccgcctga atattgcttt
      421 tttacggagt tttatggata cacctgcgat tcgttttgag tctgttagcc gccattttgg
      481 agatgtaaaa gccgtagatc aagtggaaat ggaaattcgc gatggcgaat ttttttccat
      541 gttaggacct tcaggttcag gcaaaaccac ctgtctgcga atgattgcgg ggtttgaccg
      601 acctacctct gggcatattt atttatacgg tcaagatgtt tccaatcttc cgccctatga
      661 acgggatgta aataccgttt ttcaagatta tgctcttttc cctcatatgt ctgtgggcga
      721 taatattgcc tatggtctca tgatcaaaaa ggtagaaaaa gttgagcgta tgaaacgagt
      781 ggaagaaatg ctggatctcg tccaactctc tggctttgcc ggacgcaaac cttcccaact
      841 ctcaggcgga cagcgccaac gcgtcgcact tgcccgtgcc ttgatcaatc atcccaaagt
      901 tttgctactc gatgaacctc tcggcgcatt agacttgaaa ttgcgccagc aaatgcaagt
      961 ggaattaaaa gctattcaaa aacgagtggg catcaccttt atttttgtga cccacgatca
     1021 agaagaagcg ctcaccatga gcgaccgcat tgcggttttt agtcagggga aaattgaaca
     1081 gattggtacc ccgtctgaag tgtatgaaca tcccgcgtcg cagtttgtgg cggggtttgt
     1141 gggtacatcc aatttgatta gcggagacgt cgccgaacgc atcacaggtc acgcgcagac
     1201 tttttccatc cgccctgaaa agattcatat tggttcacaa caggaataca ttagctcaga
     1261 tatgtacgcc gtggatggca ccattcgcga tgtgatttat cttggcttgt acactcgcta
     1321 tttggttgaa atcacgcaag gcatcgacct tgtggtcatt tcacaaaatt tgaaaacaac
     1381 ctctgccgac gtcttctcgt ctaagggcag tgccgtgcgg ctttcatggc aacaagacca
     1441 cattagtatt ttgggaggca aaggtgctta accgcatctc cacctatttg taccggcatc
     1501 cccgcctcgt gctagggctt ttgctcggac ctcccatgct ttatatgggt gtggtgtatt
     1561 tggggtctct gttttcgtta ttgatcaaca gcttctttta ccttgaagaa tttacaggct
     1621 tggttgttcg agaatttaca ttacaaacat acgccaaact tttagaccca accaacctag
     1681 gtattttctt tcgcacggca accatggcgc tgctagtaac cattgcagat gccattattg
     1741 cttttccgct tgcatattat attgcgcggt ttgcgccgaa aaaattaaag acctatttat
     1801 acattgccgt caccattcca ctttggtcca gctatcttgt acgtgtctac gcctggaaat
     1861 tgattcttgc caaagaaggc attgtttcct ggttctttaa cttatttggt ctgcaattta
     1921 ttttggatgg cattctttcc attcctggta ttggtggctc atctctttcg ctttctccaa
     1981 ttgggatgtt cattgcattt tgctatatct ggctgccata tatgattgtc cccattcaaa
     2041 cagcattgga acgcgtgcca aaatctttgc tcgaagcatc cagtgatttg ggcgcgaacc
     2101 cttcacaaac ttttcgccaa gtattgttac ccttgtcctt ccctggcgta gtcgcgggtt
     2161 ctatttttac attttcccta actttaggtg attttattat tcctcaagtg ctaggcaatt
     2221 cgcgcttttt tattggtcag gctgtgtatt cctatcaagg tacggctggc aacattccac
     2281 ttgctgctgc gttcacaatg ggacccatcg tcatcatggt tgtttattta ttgatcgccc
     2341 gtaaattggg agctttcgat gcgctctaag tcttcagact ctgcttctcc cgccttggtg
     2401 cttgcagcca ttggcggctt gctctttcta catgccccaa tttgggtgat cttcctgtac
     2461 tcattcacaa cagacgaagc cacatacaca tttccacttc ccggactcac aaccaaatgg
     2521 tttggcgcag cactccaacg tgtggattta tggaatgccc tcacgctttc tctgcaggtg
     2581 gcggttgtgg ctactctcgt tgctttggtt ctcggaaccc ttgctgccgc agcagtgtac
     2641 cgcagtgact tctttggcaa agaaaccatt tcgctcttgt tgattttgcc gattgccttg
     2701 cccggtattg tgacaggtat tgctttgcgt tctgccatcg gcacactcaa tattccattt
     2761 tcttattgga ccattgtgat tggtcatgcc accttttgtg ttgtgacggt ttacaacaat
     2821 gtgttagcgc gtttccgtcg gatgcaaggt tcgcaagtcg aagcctcaat ggacttgggc
     2881 gcaaattctt tccagacatt tcgctatgtc gtcttgccga acatcgccac agcgctggta
     2941 gcaggcggca tgcttgcgtt tgcactttcc ttcgacgaag tcattgttac aacatttact
     3001 gctgggcaac aggctacctt gcccatttgg attttcagcc aactcgtccg cccgcgtgac
     3061 cgccctgtga caaatgttgt agccaccatt gtggtgctta ttaccttcct cccgatcttt
     3121 ttcgcccaac gtatctctgc ttcttctgcg gatacagaag gcgattcaaa ataattggat
     3181 aaagccagaa agccgcatcg ctttctggct tccgcttctt tctcgtatcg aacctgactc
     3241 ttcctgataa ctatgtttac tcaacccaaa tcgactgcct cttacttaaa cccgaaatgc
     3301 gaagctc
//
LOCUS       DAOREF010000272         3300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18718_length_3300_cov_3.682589, whole genome shotgun sequence.
ACCESSION   DAOREF010000272 DAOREF010000000
VERSION     DAOREF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18718_length_3300_cov_3.682589"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..913)
                     /locus_tag="PKI33_06830"
     CDS             complement(167..913)
                     /locus_tag="PKI33_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNM36756.1"
                     /translation="MQALGIDIGGSGIKGAPVNIETGELLAERLRIKTPKGAKPEPVA
                     EVVAEIVRAFKWNGPIGVGFPAPIKNGVALMAANVSEKWVGTNADELLTKTTGCVCKV
                     GNDADVAGMAEMKFGAGKGQHGTVIMITLGTGIGTAIFYNGHLLPNTEFGHLQMDGKD
                     AEHRASDAARQAKDMSWKKYAKRLNEYLNEMEKLFWPDLFIVGGGISKYHEKYLPLLE
                     LQAKIVPAQFQNEAGIVGAALYSQGMDVKV"
     gene            1063..1695
                     /locus_tag="PKI33_06835"
     CDS             1063..1695
                     /locus_tag="PKI33_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNM36757.1"
                     /translation="MKKDKEEKKRFHVSDLETLRAIADPLRVQILELLEGQALTVKQV
                     AEKLGLASSKLYYHFGALEKLGLIEVAETRMVANMLEKTYRSAAHLLDVDPTLFNFSK
                     GGDNESINILLSSTIDATREDLLRSLQARQFQLEQGAEEQPRRIIINRVVSSASEKRI
                     AEFQERLVKLIQEFEEEDKTSKSSDQPYALTVAFYPSFYFDKTVKKGKKK"
     gene            1692..3047
                     /locus_tag="PKI33_06840"
     CDS             1692..3047
                     /locus_tag="PKI33_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36758.1"
                     /translation="MTSTALETTNSIETPKDMKTFFTIWVGQLISMLGSGLTNFALAV
                     WIYDQTKQATPFAITVLLGTIPRVLLLPIAGSVADRFNRRFIMIAADTCNALLTLVIF
                     LLLGAGNLQLWHIYVIAFLESVFAAFQEPAFSASISSIVPKEKLGSANGMLQMREALS
                     TVITPVLAGILFVAIGFNGIIMIDFVTFFFAVGALLLVKIPQPEQTQEHKDKNVWQDI
                     VFGWNYLRERTGLFGLILYYAMVNFLLNWSAVLLIPMVLSRFTADVLGVIQTVMGVGM
                     LVGSVIMSAWGGAKQRIPAAIGFIVLGVTGFIIAGLQPNPWLIGAGIFLLMLSVPLAS
                     GNSQVVFQTKVPREIQGRVFSVRSTISQSMMPLAFILAGPLADKFFEPLLMDGGALAN
                     TLVGQILGTGVGRGIGLMFIISGLIGIIVSLFVYLNPHIRNLESELPDALPESEKAPQ
                     A"
     gene            complement(3103..>3300)
                     /locus_tag="PKI33_06845"
     CDS             complement(3103..>3300)
                     /locus_tag="PKI33_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36759.1"
                     /translation="RFLLSRLERISADSSVAYRASGVRGSMLRMVEKLERGKPVLGPE
                     VRRLIDSAYHLLEKAAEEKIR"
BASE COUNT          812 a          780 c          778 g          930 t
ORIGIN      
        1 ttgaagaaaa gatgtctttt gtcgatatcc ccgaagatgt ggtcgaacaa gtgctaaagc
       61 acagtggaaa ttacaagatc gggaaagata aatttagcgt tgccaaagcg cagtaaataa
      121 aaaaagggac ctgatatttc tatcaggtcc ctttttgatt cgaagactaa accttcacgt
      181 ccataccttg cgagtacaac gctgcaccga cgatacctgc ttcattttga aattgtgccg
      241 gaacgatctt ggcttgtaat tccaaaagtg gcaaatattt ttcgtgatat ttgctgatac
      301 cgccgccaac aatgaacagg tcgggccaga aaagtttttc catctcgttc agatattcat
      361 tcaggcgttt ggcgtatttt ttccaagaca tatccttggc ttgacgtgcc gcatctgatg
      421 cgcggtgttc tgcatccttg ccgtccattt gcaggtgacc aaactccgta ttgggcagta
      481 aatgcccatt ataaaaaata gcagtaccaa tgcctgtgcc cagtgtgatc atgatcactg
      541 tgccatgttg ccccttgcct gcaccaaatt tcatctctgc catgcctgcc acatcggcat
      601 cgttgcctac tttacaaacg caaccggtgg tctttgtcag caattcatca gcattcgtgc
      661 ctacccattt ttctgaaacg tttgccgcca taagtgcgac tccgtttttg atgggggcgg
      721 ggaaccccac gccaatcggt ccgttccact tgaatgcacg aacgatctct gctacgactt
      781 ctgctacagg ttcgggcttt gcgcctttgg gcgttttgat gcgcaaacgt tctgcgagca
      841 attctcctgt ttcaatattg acgggtgcgc ctttgatgcc agaaccacca atgtcaatgc
      901 ctaatgcttg catgcgtcga ctcctaagtt gaggtatgag ggctatgata atgccaactt
      961 ccctaaaaag ggggaggggg aaaacccttg actctttccc gaaaataagt aaaatcagac
     1021 ttatcgttaa aaaatatttt aacgataaga ggaaattttt ctatgaaaaa agataaagaa
     1081 gagaaaaaac gctttcatgt ttccgacttg gaaaccctgc gtgccattgc agaccctttg
     1141 cgggtgcaaa tcctcgagct gctcgaaggg caggcgctaa cggttaagca ggtagcagag
     1201 aaattgggtc ttgcatccag taagctctac tatcactttg gcgcgcttga aaaactaggg
     1261 ttgatcgaag tggcagagac tcgcatggtt gccaatatgt tggagaaaac ctatcggtct
     1321 gctgctcatc ttttggatgt ggacccgact ctctttaatt tttcaaaggg cggcgataat
     1381 gaatcgatca acattttgct ttcatccact attgatgcaa cccgcgaaga tctattgcga
     1441 agtttacaag cccggcaatt ccaactggag cagggcgcag aagaacagcc gcgccgcatc
     1501 attatcaatc gcgtagtgag cagcgcttcc gaaaagcgga ttgctgaatt tcaagagcgt
     1561 ttggtgaaac tgatccaaga attcgaagag gaagataaaa cgtcaaaatc ttccgaccaa
     1621 ccttatgcat taacggtagc gttctacccg agtttttatt ttgacaagac cgtcaagaaa
     1681 ggtaaaaaga aatgacatcc accgctttag aaacaacgaa tagtattgaa acgccaaaag
     1741 atatgaagac ctttttcacg atctgggtcg gacaattaat ctccatgctc ggctctgggc
     1801 tgacgaactt cgccctcgca gtatggatct acgaccagac taagcaagca actccctttg
     1861 cgattaccgt gttattggga accattccgc gtgttttatt gctgcctatc gcaggttctg
     1921 tcgcagaccg cttcaatcga cgctttatta tgatcgctgc ggacacctgc aacgctttat
     1981 tgaccttggt gatcttcttg cttttaggcg ccgggaattt gcaactttgg catatttatg
     2041 taattgcctt tttagagtcg gtgtttgctg cttttcaaga accagcattt tctgcatcga
     2101 tctccagtat tgttcccaaa gaaaaactgg gcagtgccaa tggcatgctt caaatgcgtg
     2161 aagcgttgag cacggttatc acccccgtgt tggctggtat cttatttgtt gccattggtt
     2221 tcaatggcat catcatgatc gactttgtga ccttcttctt tgcagtgggt gcgttgttgc
     2281 tggtgaaaat tccacaacca gaacaaacgc aggaacataa agacaagaac gtttggcaag
     2341 atatcgtctt cggttggaat tatctgcgag aacgaacggg tctgtttggg ttgatccttt
     2401 attatgcaat ggtcaacttt ctgcttaact ggtctgcagt tttactcatc ccgatggtct
     2461 tgagccgctt cactgccgat gtgttgggag tgatccaaac tgtgatgggc gtgggcatgt
     2521 tagtaggcag cgttattatg agcgcatggg gcggcgcgaa acagcgcatt ccggctgcca
     2581 tcggttttat cgttctcggt gtgactggtt ttatcattgc tggtttacaa cccaaccctt
     2641 ggttgattgg cgctggtatc ttcctcttga tgctatcggt gccgcttgcg agcggcaata
     2701 gccaggttgt ttttcaaacc aaagtgccac gtgaaattca agggcgtgtc ttctctgttc
     2761 gctcgactat ttcacaatcc atgatgccgc tcgcatttat tctggcgggt ccacttgccg
     2821 ataaattttt tgagccttta ctcatggacg gtggtgcgct tgcaaatacc cttgtgggtc
     2881 agattcttgg gacgggtgtc gggcgcggca ttggtttgat gttcattatc tccggcttga
     2941 tcggcattat tgtcagtctc ttcgtatacc ttaacccgca cattcgtaat ttggaatcag
     3001 aattaccaga tgctttgcct gaatcggaaa aagcgcctca agcctgaccg ataacgggtc
     3061 aaacaaaaag agctgtatcc atagcgatgc ggctcttttt gtttaccgga ttttctcctc
     3121 agccgccttc tcaaggaggt gataagcact atcgatcaag cgcctcactt cgggacccag
     3181 aacgggtttg cccctttcaa gcttttccac catcctcagc atggaccctc tcacgccgct
     3241 ggcgcgatac gccaccgatg aatcggcgga gattcgttca aggcgagaga gcaggaagcg
//
LOCUS       DAOREF010000273         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18725_length_3299_cov_3.634402, whole genome shotgun sequence.
ACCESSION   DAOREF010000273 DAOREF010000000
VERSION     DAOREF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18725_length_3299_cov_3.634402"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /locus_tag="PKI33_06850"
     CDS             <1..2062
                     /locus_tag="PKI33_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36760.1"
                     /translation="ACVEFYNVAWGTGKAVGLFSPRWLILFVSFVLLCVFVYVCVGIA
                     VLNRERFFALIQNIVVLRNRIPILSWILAILFLTFPAWFLQRTMWGVVFDGIYFRLFL
                     WASTLFLVSFLITRGTTLVEWKSFLVALILTSSVFTIAISLQGVTDYPFSMGWSEGNR
                     IWDYSMRFGRDRYGIADNQDIYVLLDSGRMLIGGLPFLIPNVTIEMVRLWIGLTLILP
                     YFLVGIAAYIVAEKNIRIWMLMTLWVFLFLRQGPIHSPLVLVAALTIFIWRKPLWLAI
                     PLIIYAGYFAQASRFTWLFAPGLWIGMLEFAGASLSNGKLHRTHWVRTITLGLSGAFG
                     GFILPKLMLLLHVARTSSTPIDVTEQIANTGVNSEFVTYAVTDQPLLWYRLLPNSTYG
                     TGILLGLVIAVLPIILWLVWLSASKKWQLNVWQKLAIVGPLLAFLGVGLVASTKIGGG
                     GDLHNMDMFLIGVAFTVFIAWVNGGKEVVVNNEMPSWVRLTLVAALVFPAVTPLRQLR
                     SYNHGDDLTTLFVLTDKTPVEAKSFDMLPNSATVDTALTEIQDAVDEYQTLGDVLFID
                     QRQLLTFDYIHEEKFIPEYEKKVLMNEALSTNYEYYSSFYRDITNQRFSLIITEPLRT
                     PLKDSSFEFGEENNAWVEWVSIPVLCYYQEIQTYKEVNVMLLIPRPVPDNCTDYIPPK
                     PADQ"
     gene            2067..2888
                     /locus_tag="PKI33_06855"
     CDS             2067..2888
                     /locus_tag="PKI33_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36761.1"
                     /translation="MNLRYLFLRLLRHFMPEGLARFLLKRRWIIKPGLESTDPFAASA
                     RYVDTLDANGVSITGKRVLVFGYGGRFAVGVDLLKRGVAQVVLCDHFVLLDTERNREL
                     LSAYGQYLKVEGDEVQPRSEFITLLHGDIREEAIQKRISQVDIVLSTSVFEHLDDVSG
                     ITKALAKLTASQGIHLHFVDLRDHFFKYPFEMLKYSESVWKNFLNPTSNLNRYRWNDY
                     KQVFEENFGQVNITVIERLVEEFNKVKTKIRPEFKTGDENVDAVTLIHVIAKQPK"
     gene            2901..>3299
                     /locus_tag="PKI33_06860"
     CDS             2901..>3299
                     /locus_tag="PKI33_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNM36762.1"
                     /translation="MQNRLNIFFVRYEFLLPLFIFVAFLAASLPGNSWGAPALWNPDE
                     LVWRVDMALHGNMKFDVTEPDYNYPSLPKYVMYAIGYITYGLGRSTYAFIVAARSFSA
                     FLGALAGVLIYYLARLIGSNKRTATLAGVFY"
BASE COUNT          805 a          622 c          746 g         1126 t
ORIGIN      
        1 ggcatgtgtc gagttctata atgtggcgtg gggtactggc aaggctgtgg ggttattctc
       61 gccacgttgg cttatccttt ttgtttcttt tgttttacta tgcgtgtttg tatatgtatg
      121 tgtgggcatt gctgttctta accgcgaaag attttttgct ttaattcaaa acatagttgt
      181 attaagaaat agaataccaa tattaagctg gattcttgca attttatttt tgacttttcc
      241 agcgtggttt ttgcaacgca ccatgtgggg tgttgttttt gatggcattt attttcgttt
      301 gtttttatgg gcatcaacgc tttttcttgt ttcttttctg attacgcgtg gaaccacatt
      361 ggttgaatgg aaatccttcc ttgttgcatt gatactcact tcaagtgttt ttacaattgc
      421 aatttctttg caaggtgtaa cggattatcc attctctatg ggctggtctg aaggcaatcg
      481 catctgggat tattcaatgc gtttcgggcg tgatcgttat ggcattgctg acaatcagga
      541 tatttatgtt ttgcttgatt ctggtcgtat gctaataggc ggtttgccat ttttgattcc
      601 caatgtgact attgaaatgg tgcgtttatg gattgggctt actttgatct tgccctattt
      661 tttagttgga attgctgctt atattgttgc cgaaaaaaat attcgtattt ggatgttgat
      721 gactttatgg gtttttctgt ttctgcggca gggtcccatc cattctccgt tagttcttgt
      781 tgcggcatta actatcttca tctggcgtaa accattgtgg cttgcaattc cattgattat
      841 ttatgctgga tattttgccc aagcgagtcg atttacctgg ttgttcgctc ccggtctatg
      901 gatcggaatg cttgaattcg caggcgcttc tttgtctaat ggaaaactgc atcgaaccca
      961 ttgggttcgg actatcactt taggcttgtc cggcgcattt ggcggattca ttttgccaaa
     1021 attgatgtta cttctgcatg tggcacgaac ttcttcgaca ccaatcgatg ttacagaaca
     1081 aattgccaat actggcgtca attcagagtt tgtaacctat gctgtgacag accaacccct
     1141 cttatggtat cgcctactgc ctaactcaac ctatggaact ggcattttgc ttgggttggt
     1201 cattgctgta ttgccgatta ttctttggtt ggtctggctt tctgcctcta aaaagtggca
     1261 attaaatgtt tggcaaaaac ttgctattgt tggacctctc cttgccttcc ttggagtagg
     1321 cttggttgcc agcacaaaga tcggtggcgg cggcgaccta cataatatgg atatgtttct
     1381 gataggcgtt gcttttacag tttttatagc ttgggtcaat ggcggtaaag aggtggttgt
     1441 gaacaatgaa atgccaagct gggtgcggct tacacttgtt gctgcattgg ttttccccgc
     1501 tgtaacaccc ctccgccagt tgcgttcata caatcatggc gatgacttaa ctaccttatt
     1561 cgtcctgacc gataaaaccc cggtggaagc caaatctttt gatatgttgc ctaatagtgc
     1621 tacagttgat accgctttga ccgaaattca agatgcggtg gatgagtatc aaaccctagg
     1681 agatgtttta ttcatagacc agcgccaatt gttgacattt gactatattc atgaagagaa
     1741 gtttatccct gagtatgaaa agaaagttct tatgaatgag gcgctaagta cgaactacga
     1801 atattattcg tctttctaca gagacattac caatcagcgc ttttcactaa ttattactga
     1861 acccttgcga acccctctca aagatagctc ctttgaattt ggcgaagaga acaatgcttg
     1921 ggttgaatgg gtatccattc ctgtactctg ttattatcaa gagatacaga cctacaaaga
     1981 ggttaatgta atgttgctta ttccaagacc agtgccagat aattgcacag actatattcc
     2041 gccaaaacct gctgatcaat agacttatga accttcgcta ccttttcctt cgacttttac
     2101 gccattttat gccagaaggg ctggcacgct tcttacttaa acgacgctgg atcattaagc
     2161 ccggacttga atccaccgat ccattcgccg cttccgcgcg ctatgttgat acattagatg
     2221 caaatggagt gtccattact ggaaaacgag tgctggtctt tgggtatggt ggacgttttg
     2281 ctgttggggt agacttactc aagcgtggtg tggcgcaggt agtcttgtgc gatcattttg
     2341 tattgttgga tacagaacgt aaccgcgaat tgctgtcagc gtatggacaa tacctgaaag
     2401 tggaggggga tgaggttcag ccacgaagcg aatttattac cttgttgcat ggtgacatcc
     2461 gtgaagaagc catccaaaaa cggatttcac aggttgatat tgtcttaagc acttcggtct
     2521 ttgaacattt ggatgatgtg tcaggaatta caaaagcgct tgcaaaacta actgcgtcac
     2581 aaggcattca tttgcatttt gtggacctgc gcgaccattt ctttaagtat ccatttgaga
     2641 tgttgaagta ttcggaaagt gtttggaaga acttcttaaa tcccactagt aatttgaatc
     2701 gttatcgttg gaatgactat aagcaggtct ttgaagagaa ctttggacaa gtaaatatta
     2761 cggtgattga acggcttgtt gaagaattta acaaagtgaa aactaaaatc cgccctgaat
     2821 ttaaaactgg ggatgaaaat gtggatgcgg tgactcttat tcatgtaata gcgaaacagc
     2881 caaagtgatt ggagtttaag atgcaaaata gattaaatat attttttgtg cggtatgaat
     2941 ttttactgcc actttttatt ttcgttgctt ttcttgctgc ttcattacct ggtaatagtt
     3001 ggggcgcccc tgctttatgg aatcctgatg aattggtttg gcgtgtagat atggcattac
     3061 atggcaatat gaaatttgat gttaccgagc cagattataa ctatccttct ttgccaaaat
     3121 acgtgatgta tgcaattgga tatattacat acggattagg acgttctacg tatgctttta
     3181 ttgtagctgc gagatcattt tcggcatttt taggtgcttt agcaggggtg ctgatctatt
     3241 accttgcccg cctaattggt tctaataaaa gaactgctac tttagcagga gtgttttac
//
LOCUS       DAOREF010000274         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18768_length_3294_cov_4.372028, whole genome shotgun sequence.
ACCESSION   DAOREF010000274 DAOREF010000000
VERSION     DAOREF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18768_length_3294_cov_4.372028"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKI33_06865"
     CDS             complement(<1..532)
                     /locus_tag="PKI33_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36763.1"
                     /translation="MRIITAIIAIVVGILVLLGYFLPQAAIFQVILLDWAIILAGAAT
                     LAGVLNLVTVHSEKIRTRSKDNIYSAILILGMFATFVFSLLLKPQNIYVQTFILNSII
                     VPVEASLMALLAVSLLYAAVRLLRRRADLMGIVFVLTAALLFFGSATLPFGDVPIMGT
                     LVRPWVSQILALGGARG"
     gene            complement(532..1263)
                     /locus_tag="PKI33_06870"
     CDS             complement(532..1263)
                     /locus_tag="PKI33_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36764.1"
                     /translation="MTALAILGVAAFLLLSLTLWKRRSPSYLREIPAFTKLAHSLGLS
                     IEDGKRLHISLGHGGLLDAHGGSAFAGLALLRNIAERTSVSDMPAVASAGDPTLGLLS
                     QDTLQAGYQAAGVEDAYVHTTGRVTGLSPFSYAAGAMQIPQNENVSTNIVIGHLGAEA
                     GLITDAAERNNIKLIGSSDNLVGQSILFANTQDPLIGEELFAAGAYLNEDPAHAASLT
                     VQDIFRWLIILILLGGAALKFLGTL"
     gene            complement(1260..1505)
                     /locus_tag="PKI33_06875"
     CDS             complement(1260..1505)
                     /locus_tag="PKI33_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36765.1"
                     /translation="MKSTREFWQRWAESLRRYQLHDLVASFLEAGSPLALLGAQAIYF
                     GGGLVKNDQLTALAGLLEDENETRAFVAFLSHNGASS"
     gene            complement(1629..2879)
                     /locus_tag="PKI33_06880"
     CDS             complement(1629..2879)
                     /locus_tag="PKI33_06880"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNM36766.1"
                     /translation="MPSYNEVLAVILGGGRGARLYPLTQMRSKPAVPIAGKYRLIDIP
                     ISNCINSEIFRIAILTQFNSVSLHRHITRTYNFDSFHQGWVQIWAAEQTIESADWYQG
                     TADAVRKQMLEIQATGAKYVLILAGDHLYRMDYKAMAEFHWANNADITVAVQPVAKDE
                     ANRFGVLKRESDGTISDFYEKPKDPDVQKRFVSRDDPERPFLGSMGIYMFNTKVLLDF
                     ISNFPDYDDFGGDIIPQAIKTHKVFGFDFDGYWQDIGTIRSFYETNLMLTKSSTPFDF
                     YDAKLPIYTDTRYLPSSIVEDSQMKDVLIAEGSRVLKSEITHSIIGIRSQIAGGCVIK
                     DSIVMGSDYYEREHRAGTPLGIGPNCHIEGAILDKNVHIGENVTIRPFPRNTDIDSEK
                     WYVRDGIVIIPKDVEIPSGTVIAP"
BASE COUNT          937 a          772 c          793 g          792 t
ORIGIN      
        1 tgccgcgtgc cccacctagc gccaaaatct gagagaccca agggcgcacc aaggtgccca
       61 taatgggaac gtcaccgaaa ggcaatgtgg cagagccaaa gaaaagcaaa gctgctgtta
      121 gaacaaaaac tattcccatc aagtctgctc ggcggcgcaa taagcggact gcagcatata
      181 acaatgaaac tgccagcaac gccataagag aggcttccac tggaacaatg atgctattca
      241 ggataaaggt ttgcacataa atattttgcg gcttgagcaa caaagaaaac acaaatgtag
      301 caaacatgcc caagatcaaa atagcactat agatattgtc ttttgaacga gtgcgaatct
      361 tttctgagtg aactgtaacg agattaagga cccccgccaa ggttgctgcc cctgccagga
      421 taattgccca atcgagcaag atgacttgaa agatcgcggc ttgcggcagg aaatatccca
      481 gcaagacgag gataccgaca acaatggcga taatggcagt gataatgcgc attagagcgt
      541 ccctaagaat ttaagtgctg ccccacccaa aaggatgagg atgatcagcc agcggaaaat
      601 atcttggaca gtgaggctgg cagcatgggc agggtcttca ttcagatagg cgcctgcagc
      661 aaagagttct tcaccgatca gtgggtcttg ggtattggca aaaagaatgg actgccccac
      721 cagattatca gacgagccaa tcagtttgat gttgttgcgt tcggcagcat ctgtaataag
      781 cccggcttct gctcccaaat gaccaatgac tatgttcgta gaaacattct cgttttgagg
      841 aatttgcatt gcaccagccg cataactaaa tggtgataga cccgtaacac gcccagtggt
      901 gtggacgtag gcatcttcga cgccggcggc ttggtatccg gcttgcaagg tgtcttggga
      961 taacagacca agtgtcgggt cccctgccga ggcgacagca ggcatatcac tcacagaggt
     1021 gcgctcagca atattccgca gtagagcaag cccggcgaag gcagatccgc cgtgtgcatc
     1081 gagcaggccg ccatgcccaa gcgaaatgtg aaggcgtttt ccgtcttcga tgcttagtcc
     1141 aagagaatga gcaagttttg taaaagcggg aatttcacgt aaatatgagg gagagcgtcg
     1201 cttccataat gtaaggctaa gtaataagaa agctgcgacg ccaagaatag ctagtgcggt
     1261 catgaactcg ctccattgtg actcaaaaaa gcgacaaagg ctcgtgtctc gttttcatct
     1321 tcaagcaaac cggcaagagc tgtaagttgg tcgttcttga caagtccccc accaaaataa
     1381 atcgcctgag caccgagcag ggcaagcggg cttcctgctt ctagaaatga ggcgacaaga
     1441 tcgtgaagtt gataacggcg caaggactca gcccagcgtt gccagaattc gcgcgttgac
     1501 ttcatagtta atgcgagtat agcataattt taaggttgct cctgctgcat aagagcgcta
     1561 aatgttacag ttttgtgaat atttcaagaa aaaacccctg aagaattcga tcttcagggg
     1621 ttcattgttt atggagcgat gaccgtaccc gatggtatct ctacatcttt tggaatgatg
     1681 acaataccat ctcgcacata ccatttttcg ctgtcgatat cggtattgcg tgggaagggg
     1741 cgaatggtta cattctcgcc aatgtgtaca ttcttatcga gaatggcacc ttcaatgtgg
     1801 caatttggtc caattcctaa gggcgtgcct gcacggtgtt cacgttcata gtaatcagaa
     1861 cccatcacaa tgctgtcttt gatcacacag ccaccagcga tctgactgcg aataccaatg
     1921 atcgaatgcg tgatctcaga tttcaagacc cggctacctt cagcgatcaa tacatctttc
     1981 atctggctat cttccacaat ggaagaaggc agataacgcg tatcggtata gatcggcagt
     2041 tttgcatcgt agaaatcaaa cggagtactt gatttcgtca gcatcaaatt ggtctcataa
     2101 aacgagcgga tcgttccaat gtcttgccag tagccgtcaa aatcaaagcc aaagacttta
     2161 tgggtcttaa tcgcttgcgg aataatatcg ccgccaaagt catcgtaatc tgggaagttg
     2221 ctgataaagt ccaacagtac tttggtgttg aacatataga tacccatgga accgagaaaa
     2281 ggtcgttcag ggtcatcacg gctgacaaat cttttttgga cgtctggatc tttaggcttt
     2341 tcgtaaaaat ctgagatcgt accgtctgat tcacgcttca agacgccgaa acgatttgct
     2401 tcgtccttcg caaccggttg cacggcgacg gtgatatctg cattattcgc ccagtgaaac
     2461 tcagccattg ccttgtagtc catacggtac agatgatccc ctgcaaggat gagtacatac
     2521 tttgcacccg tggcttgaat ttcaagcatc tgcttgcgaa cagcatcggc tgtgccttga
     2581 taccagtcgg cactttctat cgtctgttcg gctgcccaga tctgcaccca accctgatgg
     2641 aaagaatcaa aattataggt gcgggtgata tgacgatgca atgagaccga gttgaactgg
     2701 gtcagaatcg caatacgaaa tatctctgag ttaatacaat tactaatggg aatgtcaatg
     2761 agacggtatt ttcccgcaat cgggacggca ggcttagatc tcatttgtgt gagcgggtaa
     2821 aggcgtgccc cgcgtccacc cccaaggatc acagccagga cttcgttgta cgacggcata
     2881 tggagcctcc tggaattttc gttcaggaat gtcgagcaat gctccctatt ttacaccctt
     2941 caccttacga atgcgatgaa ttacataagc aattgggtat gccaaaatca cgaaaacatc
     3001 aaacaacatg aaaaagagag aaagtttcat gagggactcc tcctacgggt ctagacagct
     3061 taatataaat acgtataaaa cccaaacttg tttctatttt cattctacaa tccccaaatg
     3121 acccggtcaa ccttacagag tagggtacat ataaaaatca aacgtcctcc attgcggagg
     3181 acgctgccag aggtggatta tttgctctta gggtcaagta cccgtagggt ataatccgtg
     3241 tgccgaagga gggaatcgaa ccctcattcc cggagggaac acgattttga gtcg
//
LOCUS       DAOREF010000275         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18769_length_3294_cov_3.611300, whole genome shotgun sequence.
ACCESSION   DAOREF010000275 DAOREF010000000
VERSION     DAOREF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18769_length_3294_cov_3.611300"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKI33_06885"
     CDS             <1..470
                     /locus_tag="PKI33_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNM36767.1"
                     /translation="DGGVDLLIIETMFAVEETTAAFEGARSVTDLPIVVSFSYDRGTR
                     TMMGVKPKDVMKRYSEMGATLIGANCGTTLDNMEAVVKEYQTAKADMPLWVKPNAGVP
                     HMDLETEQGVYDMGPEDMATYAKKYVAMGAKVVGGCCGNTPEHIAAIVKAVKN"
     gene            573..1550
                     /locus_tag="PKI33_06890"
     CDS             573..1550
                     /locus_tag="PKI33_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNM36768.1"
                     /translation="MRDEIMKLLNGEKISSVPAFSGLIHVTAEGLASEGLSLDEVHHD
                     AEKMARAAASTFKLTGMPSATLPLDLCSPAEALGAELIFYNNEEMQFPQVRKLLFMSA
                     REVAQIQPTLGGRIPLICDAIRLTKKNIGEHAVISGMIPGPYTLMIYICKVQNLIREM
                     KNEPQMVLDALTHLSSFLADIGNAYLDAGADFITIHEMGGSPGFIGPKPFEGFVLPAL
                     QHLTSSLPHPTVLSVCGKTDSAMSLLAQSGADAISVDQLNDLKASRSVLKNKLLFGNV
                     DPVATLWQGNEGSVAETVQRSKEAGVDAVWPGCDMVIQTPVQNIKNMLG"
     gene            1625..>3294
                     /locus_tag="PKI33_06895"
     CDS             1625..>3294
                     /locus_tag="PKI33_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36769.1"
                     /translation="MAKKSSSVGKVLTKDRPAKAASKKRLSVSGSLSLSSAFPEVQEL
                     ERTGQHAAAIDLASQKLSSESDVDLRMTLLGLRCDSYIALGKFGLAEADANAMRELAD
                     PSDPELEIRALKHQASVMLVKGELVEAAQTAGQAVAQARALKPPNPYLLAQCLYMLGD
                     CHKNSGNYENAIVAAKEAIPLFEATGSVKETGRTHWLMAPSYNVLGNAKKSREAAQNA
                     LNLCQQAGDSMGVGNAYNALSSTDTDIAERIQHYQKAIQAFEISGYLERVTGAIGNLG
                     FTYAALGLYMHSCRLTNKALEIDRSMGAKLGMSYWLENLFDAELRLGWVDLARQHLNE
                     FTALTPDLGSSRVTVSQHMNFGLMALGEEKPAEAVEHFQTAVELARESNAGNLNVALT
                     YLSHAHLVHKNPVAALEASTQATELHRQQGYKLPDSFSSQEIWWRHTQALLANGKTTA
                     ARAALERAYKFLLEAITNVRDEGLRRNYLNKVQENRDLLNFWVKDSLKHKLTRKRIFA
                     HLFIESNTREPFKRLTDTSLRLNALHAVEDIHTFIVEEATELSGGERVL"
BASE COUNT          870 a          821 c          879 g          724 t
ORIGIN      
        1 ccgatggcgg cgtggacttg ttgatcatcg aaaccatgtt cgccgtggaa gaaacaactg
       61 ccgcctttga aggcgcgcgc tcggtgactg acctgccgat cgtggtgtca ttcagctacg
      121 accgcggcac acgtaccatg atgggcgtca agccgaagga tgtgatgaag cgttactccg
      181 agatgggcgc aaccctcatc ggcgcgaact gcggcaccac cctcgacaac atggaagcgg
      241 ttgtaaaaga atatcaaacc gccaaagccg atatgccctt gtgggtcaag ccgaatgcag
      301 gcgtgccaca catggacttg gaaaccgagc aaggcgtata tgacatgggt cccgaagaca
      361 tggcaactta tgccaagaag tatgtagcca tgggtgccaa agtggtcggc ggttgctgcg
      421 ggaatactcc cgaacatatt gcggcaattg tgaaagcagt aaagaactag taaccagtaa
      481 acagttgcca gtatccagtg aaagacattt gttacggatt acgaattacg gaacacgtaa
      541 gcgtaatccg taattcgtaa gtggaggcag tgatgcgtga tgagattatg aagttgttga
      601 acggggaaaa gatttcttca gttccagcgt tcagcgggtt gattcacgtc acagccgagg
      661 gattggcgag tgaaggcttg tcgttggacg aagtccacca tgatgcagaa aagatggcac
      721 gcgcagccgc gagcacattt aagctcacag gcatgccttc ggcaacactc ccattagatc
      781 tatgctcccc tgctgaagcg ctcggcgcgg agttgatctt ctataacaat gaagagatgc
      841 aatttccgca ggtgcgtaaa ctgctgttca tgtctgcgcg cgaagtggct cagatacaac
      901 ccaccttagg cgggcggatt ccactgatct gcgatgcaat tcgattgacg aaaaaaaaca
      961 tcggcgaaca cgccgtcatt tcgggcatga ttcccggtcc gtacacgctg atgatctaca
     1021 tttgcaaggt gcagaatctc atccgcgaga tgaagaacga gccgcaaatg gttttggatg
     1081 cgctcacgca tctctcttct tttctggcag atattggcaa tgcctatttg gatgcaggcg
     1141 cagactttat caccatccac gagatgggcg gctcaccggg atttatcgga ccgaagccgt
     1201 ttgagggatt cgtcctgcct gcgcttcaac acctaacgtc aagcctgccc caccccaccg
     1261 tgctttcggt ctgcgggaaa acggactctg ccatgagtct gctggcgcaa tcgggcgcgg
     1321 atgcgatctc cgttgaccag ttgaacgacc tgaaggcttc acgctctgtg ctaaaaaata
     1381 aattgctgtt cggcaacgtc gacccggtgg cgactctgtg gcagggtaac gaaggctcgg
     1441 ttgcagagac agttcaaagg tcgaaagaag cgggcgtgga tgcggtctgg cccggttgtg
     1501 atatggtcat ccaaacgcca gtccagaata tcaaaaacat gctcgggtag ccgaaccttt
     1561 tctgggggaa cagtttacta agggtatata attgcccaat catcagagac cgaggagcaa
     1621 cggaatggca aaaaagtcat catccgtagg caaggtatta acgaaagacc gcccggcgaa
     1681 agctgcaagc aaaaaaagac tgtcggtttc aggcagtctt tcgttatcgt ctgcttttcc
     1741 tgaggttcaa gaactggaac gcaccggtca acatgcggcg gcaattgacc ttgcttctca
     1801 aaaattaagc tcagaatctg atgtcgattt aagaatgacc ctgctgggtc tgcgatgtga
     1861 ctcatatatc gcccttggaa aattcggtct cgcagaagct gatgcaaacg ccatgcgtga
     1921 gttggcggac ccaagcgacc ctgagttgga gatccgcgcg cttaagcatc aagctagtgt
     1981 gatgttagtc aaaggcgagc tggttgaagc cgctcaaact gcaggacagg cagtggcaca
     2041 agcacgtgcc ttgaaaccgc ctaacccgta tcttctagca cagtgcctgt atatgctggg
     2101 agattgccat aaaaattcgg ggaattatga aaacgccatt gtcgcagcaa aggaagccat
     2161 tccgctgttc gaagcgacag gtagtgtaaa agagactggt cgcacacatt ggttgatggc
     2221 tccttcttat aatgtattgg ggaatgcgaa gaagtcgcgc gaggcagcgc aaaatgcatt
     2281 gaacctttgc caacaagccg gtgactcgat gggggttgga aatgcctaca atgcgctcag
     2341 ttccaccgat accgatattg cagagagaat tcaacactat caaaaagcca ttcaagcttt
     2401 tgagatcagc ggatatttgg aacgcgtgac cggcgcgatc ggaaacctcg ggtttaccta
     2461 tgcagcgttg ggcttatata tgcattcctg ccgcctcacc aacaaagcgc ttgaaatcga
     2521 tagaagcatg ggcgcgaaat tgggcatgtc gtactggctc gaaaatctat tcgatgcaga
     2581 attacggctg gggtgggtag acctagctcg acagcacctg aacgagttca cggctcttac
     2641 ccctgattta ggcagttcgc gcgttaccgt tagtcagcat atgaactttg ggttgatggc
     2701 tctcggcgag gaaaagcctg ctgaagcagt tgagcacttt caaaccgcag tggagttggc
     2761 acgcgaatca aatgccggca acttgaatgt ggcactcacc tacctttctc atgcccacct
     2821 tgtacacaag aacccagttg cagcattaga ggcatcaacc caggcaaccg aattgcaccg
     2881 ccagcaaggc tataaactgc ccgatagctt cagctcacaa gagatctggt ggcgacacac
     2941 ccaagcacta ttggcaaatg gaaaaactac tgctgcccgt gctgccttgg aacgtgccta
     3001 caaattcctg ctggaagcca ttacgaatgt acgggatgaa ggattgcggc gcaattatct
     3061 caacaaggta caagagaacc gcgacctgct caatttctgg gtgaaagaca gcctcaaaca
     3121 caaactgacg cgcaaacgca tttttgccca tctcttcatt gaatccaata cccgcgagcc
     3181 gttcaaacgc ctaaccgaca ccagcctacg cctgaatgct ttgcatgcag ttgaagatat
     3241 tcatactttc attgtggaag aagccaccga gctgagcggc ggtgaacggg tgct
//
LOCUS       DAOREF010000276         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18778_length_3293_cov_5.689314, whole genome shotgun sequence.
ACCESSION   DAOREF010000276 DAOREF010000000
VERSION     DAOREF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18778_length_3293_cov_5.689314"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKI33_06900"
     CDS             complement(<1..353)
                     /locus_tag="PKI33_06900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNM36770.1"
                     /translation="MPISSIYSKPITIPSSAIDENGHVNNVVYVQWMQDIAIEHYASI
                     GGVEAQGPDATWVIRKHSIEYFLPAFEGEEIEVRTWVEDLRKVRSLRMYEFVRKSDGK
                     TLVKGESDWVFMDMKT"
     gene            453..872
                     /locus_tag="PKI33_06905"
     CDS             453..872
                     /locus_tag="PKI33_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36771.1"
                     /translation="MGPIIGGTSLIITVISIACGCLITIVTLAGTGFLIYKVFGSTFK
                     SMSTNRNVLQTGVSAPAVILSVQDTGVTMNDNPQARIRLRVMPMNGEAFEAEVTQIIG
                     RFQVGMVVPNGSAMVKYDPNDKTKVAIESFGMPSAGQ"
     gene            complement(921..1538)
                     /locus_tag="PKI33_06910"
     CDS             complement(921..1538)
                     /locus_tag="PKI33_06910"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM36772.1"
                     /translation="MKSRTILISLILVIGAFLFSCANNQADQTTALDTDAIRTQAVET
                     YASNLTQLAPSSTPFPTLTPVGITATALSLTPEPSPTSNPCFNLLYIDDVTVPDGTRM
                     KPGEKFTKTWLVQNIGGCAWRAGFTFQNVGGDSMRGTPITLTEAIQTGAKRELSVELV
                     VPSGINGVIESAWQMADDTGVFFGDTLFVSIVVGDVTTPAVTSTP"
     gene            complement(1540..1941)
                     /locus_tag="PKI33_06915"
     CDS             complement(1540..1941)
                     /locus_tag="PKI33_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36773.1"
                     /translation="MKIRDASGWTMFIFGVMAFLLGILGIIRPELQLAMLGFEVVERA
                     ARASSDYTLTFMTAASMASLNMGIYYILAALNDVKIFYRWTVPFRGLTFLVFTTVVIL
                     GIAPLKFIGVPIWELTGAIATGIALAREKQK"
     gene            2018..2668
                     /locus_tag="PKI33_06920"
     CDS             2018..2668
                     /locus_tag="PKI33_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNM36774.1"
                     /translation="MAKHLVLVAGNIGAGKTTLTERIGARLGWWTGYESVADNPYLSD
                     FYGDMRSWSFHLQVFFLGHRADQYLEAARDARSAILDRSIYEDAHIFARALHHLGNLN
                     ERDYLSYRKLFELVVNGLPRPDLLIHLKAPVPVLMDRIRRRARNMETGISAEYLTLLE
                     SFYDEWLGSFDLCPVLTIQTDDLDYVNYPQHLDQVAQRIQDKLAGREMMDLRGDTK"
     gene            2668..>3293
                     /locus_tag="PKI33_06925"
     CDS             2668..>3293
                     /locus_tag="PKI33_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNM36775.1"
                     /translation="MNEKMDSSVFLKIPLFKDLPKAELYSLVSELPVEKYQAGDYIFH
                     EGEPGNSLYVVQKGKIDVVLAGGTNDEMLLRTCGPGEYVGEMSLILKQGKRTAAVRVR
                     EDSQLWMMTRDKFNECLQRWPHLAYAMVEIISERLDSSNEAAFNDLVKKNQMLQKAYD
                     DLKAAQAQLIEKERLERELQLAADIQLSILPDVLPDTEEFGFGARILPA"
BASE COUNT          856 a          714 c          844 g          879 t
ORIGIN      
        1 gttttcatat ccataaaaac ccagtcactt tcgcctttta ctaaagtctt cccatcactc
       61 ttgcggacaa attcatacat gcgcaacgac cgtactttgc gtaaatcctc tacccaagtg
      121 cggacctcaa tctcttcgcc ttcgaaggct ggtagaaaat attcgatgct atgtttacgg
      181 atcacccagg tggcgtcagg tccctgggct tctacgcctc cgatggatgc ataatgttca
      241 atggcaatat cctgcatcca ttgcacatag acaacattgt tgacgtgtcc attctcgtca
      301 atagcagaag aggggattgt gattggtttg gagtaaatag aggaaatcgg cattttttga
      361 ttttacagtg acttccccca aacgggggag tttattttta ttgccccgct gatagaatga
      421 atttaataaa aatcattttt tggagaaatt aaatgggacc aatcattggt ggcacttcat
      481 taataattac tgtaatttca atcgcttgtg gatgtttgat taccattgtc acactggctg
      541 gaactggttt tctgatctac aaggtatttg gaagtacgtt caagagcatg tcaacaaatc
      601 gcaatgttct tcagactggc gtttctgctc cggcggtgat tctttctgtg caagacacgg
      661 gtgtgaccat gaacgataat ccacaggctc gcatccgttt gcgtgtgatg ccgatgaacg
      721 gcgaagcatt tgaagcagaa gttacccaga tcatagggcg ttttcaggtg ggaatggtcg
      781 ttccgaatgg ctcggcaatg gttaaatatg acccgaacga taaaaccaaa gtagcaattg
      841 agtccttcgg tatgccatca gccgggcaat aattctagaa gaaacaaaaa ggcgtggatt
      901 ttatccacgc ctttttgttt ttacggtgta cttgtcacgg ctggagtggt tacgtcacct
      961 acgacgatgc tgacaaacaa tgtatcgcca aagaagacac cggtatcgtc tgccatttgc
     1021 caggcgcttt cgatgacacc attaatgcca ctaggcacaa caagttctac agaaagttcg
     1081 cgtttagcgc cggtttggat ggcttcggta agagtgatgg gagttccacg catagagtcg
     1141 ccgccaacgt tttggaaggt gaaaccagca cgccatgcac aaccaccgat gttttgcacc
     1201 aaccatgttt tagtaaattt ttcgccgggt ttcatccgtg tcccatctgg gacggtgaca
     1261 tcgtcaatgt acaacaggtt gaagcaggga ttcgatgtgg gggatggctc aggggtaagg
     1321 gaaagcgccg ttgctgtgat gccaacaggc gtcagtgtag gaaatggtgt ggaggatgga
     1381 gcaagctggg tcaggtttga ggcgtatgtt tccacggctt gggtgcggat ggcatccgta
     1441 tccagtgcgg tagtttgatc tgcctgatta ttggcgcacg aaaaaaggaa tgcgccgatg
     1501 accaagatca ggctgatgag gatggttctt gatttcattc tatttttgtt tctctcttgc
     1561 cagtgcaatg ccggtggcaa ttgcgccggt cagttcccag atgggtacgc cgataaattt
     1621 aagcggggca attccgagaa tgacgacagt ggtgaataca aggaaggtca gcccgcgaaa
     1681 agggacggtc caacggtaga agatttttac atcgttcaat gcggcgagga tgtagtagat
     1741 gcccatgttt aaggatgcca tggatgccgc ggtcatgaag gtgagggtgt agtctgagct
     1801 ggcacgtgcg gcacgctcaa cgacttcgaa gccgagcatg gcaagttgaa gctccgggcg
     1861 gatgatgcca aggatgccta gtaaaaatgc catgacacca aaaataaaca tggtccagcc
     1921 ggaagcgtcg cgaattttca tggatacaga ctcctgtgtg tgcagtgata acggaaatta
     1981 taaccgcaac cattgggcat gataaaatca ctaaactatg gctaaacatc ttgtgttggt
     2041 ggcaggaaac attggtgcag gcaaaactac gttgacagaa cgtattggtg cacggcttgg
     2101 ctggtggacc ggatatgaat ctgtagctga taacccatat ctctctgatt tttatggaga
     2161 tatgcggtcg tggtcatttc acttgcaagt tttctttttg gggcatcgtg ctgatcaata
     2221 ccttgaggcg gcacgagatg ctcgttctgc gattcttgac cgtagtattt atgaagatgc
     2281 tcatattttt gcacgtgcct tgcatcattt aggaaatttg aacgaacggg attatctttc
     2341 gtatcgcaag ttgtttgaat tggtcgtgaa tggacttcca cgcccagacc tgttgattca
     2401 cctcaaagcg ccagttccag tcttgatgga ccgcattcgc cgccgtgcac gtaacatgga
     2461 aaccggcatt tctgctgaat accttacctt actcgagtca ttctatgacg aatggctcgg
     2521 ctctttcgac ctttgtcctg tgcttaccat ccaaacagat gacctcgatt atgtaaatta
     2581 tcctcaacat cttgaccagg ttgcacaacg tattcaagat aaattggcag gtagagaaat
     2641 gatggatttg cgtggagata ccaaataatg aatgaaaaga tggatagttc cgtctttcta
     2701 aagattccac tgttcaaaga cctgcccaag gcggagttgt attccttggt cagcgaactg
     2761 ccggtagaaa aatatcaagc gggcgattac atcttccatg aaggtgagcc gggcaatagt
     2821 ttatacgtgg ttcaaaaagg caagatcgac gtggttctgg cgggcggcac gaacgatgaa
     2881 atgcttctac gcacctgtgg acccggcgaa tatgtgggtg agatgagtct cattctcaaa
     2941 caaggcaagc gcacagccgc tgtccgcgtg cgggaagata gccagctttg gatgatgacc
     3001 cgcgataagt tcaatgaatg tctgcaacgc tggcctcatt tggcatatgc catggtcgag
     3061 atcatcagcg aacgtttgga ctcttcgaac gaagcagctt ttaacgatct tgtgaaaaag
     3121 aatcagatgc tgcaaaaagc ctatgatgac ttgaaagcag cccaagccca gttgatcgaa
     3181 aaagaacgcc tcgaacgtga acttcaattg gcagctgata ttcagcttag cattctccca
     3241 gacgttttgc ccgataccga agagtttggt ttcggcgcac ggattttgcc tgc
//
LOCUS       DAOREF010000277         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18839_length_3286_cov_4.625503, whole genome shotgun sequence.
ACCESSION   DAOREF010000277 DAOREF010000000
VERSION     DAOREF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18839_length_3286_cov_4.625503"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PKI33_06930"
     CDS             <1..543
                     /locus_tag="PKI33_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36776.1"
                     /translation="MDVMMPRLDGFGATQRLREFTNVPIVILTARGEEQDRVRGLDLG
                     ADDYLIKPFSATELLARVRAVLRRAHNPTDAGQVRFFTHDDLKIDFARAEVWRGEEAV
                     SLSATEYRLLLQFAHNVGKILTSEELLTSVWGVEYKNDKEILWVSIARLRQKLEKDAH
                     TPRHIVTRSGLGYLMPPIET"
     gene            582..1730
                     /locus_tag="PKI33_06935"
     CDS             582..1730
                     /locus_tag="PKI33_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36777.1"
                     /translation="MSQKKILIVDSDVASRNFIARKLQELDYETLQAGSGKEGLIYTW
                     RDHPDLVIADPALTDIKGEEFATKLRNDPRTANLPIVALSSDHNPARIKSCQDAGFND
                     YITKSGQAVPMLKDIINRLFGVTQEDMKQGGLMIVFLSAKGGTGTSSVCANTAVNITK
                     DQPEARIAVVDLVFPIGSIAQIVGYEGTDNIVTVADRNPLETDVDFLKTLPRIEPWNF
                     NLLAGSPDPELSNQLNVGRIWDIITGLKSSYDYVLIDIGRSLSKITLPLIQHADLISL
                     LISTDVSTISLTKTILDFLKSKGVKPEAIYTILNRHASLEGLSKREAETILEKNVNTA
                     IPYLGTNFGFANSQHRPFTLKFPNDTASIIFHDSAREMANLAKKIRSQ"
     gene            1763..2056
                     /locus_tag="PKI33_06940"
     CDS             1763..2056
                     /locus_tag="PKI33_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36778.1"
                     /translation="MSIEYDDKGKYYTNVIQKVPVPAMVQTTTNLIRGIVHVRHGERL
                     KDEIEGSEQFVAMTDASVYSADGKELYTGPFLALQKGQIVWVMPLNETQKGEE"
     gene            2053..>3286
                     /locus_tag="PKI33_06945"
     CDS             2053..>3286
                     /locus_tag="PKI33_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNM36779.1"
                     /translation="MNTPLSGAPQRLTGQDLIRLKKYLADNLLRALETEGIEASQRVT
                     YIQQNIGRVFDTTQLKFPEDLKREIFKQVLNDLLGFGPIQPLLDDPDISEIMVNGPKK
                     IFIEKKGQLTKSEITFDDDDHVLRVIDRIIMPLGRRVDFDSPTVDARLPDGSRVNAVV
                     RPVSIDGPSITIRKFRKDKLKAEDLIAFGSITKQMADFLEACVKARFNIVISGGTGSG
                     KTTLLNVVSGFIPEGERIVTIEDAAELQLQQDHVMRMETKPANSDGLHAVTIRDLVKN
                     SLRMRPDRIVVGEVRGGEALDMLQAMNTGHDGSLTTVHSNSPRDAISRLETLVLMAGM
                     ELPLKVVRTQISSAIDLIVQQTRLKDGKRKVTAITEVAGMEGDVVVLTDIFKFNQTGV
                     TQDGQVLGELQPTGIRPNF"
BASE COUNT          909 a          723 c          786 g          868 t
ORIGIN      
        1 atggatgtga tgatgcccag gctggatggc tttggtgcca cccaacgttt gcgcgaattc
       61 accaacgtgc ctattgtgat cctaacggcg cgcggtgaag aacaagatcg tgtacgtggt
      121 ctcgaccttg gcgcagatga ttaccttatc aagccttttt cggctacaga attgcttgct
      181 cgtgttcgcg ctgttttacg tcgcgcccac aacccaaccg atgctggtca ggtccgcttc
      241 tttacgcatg atgacctgaa gatagacttt gctcgtgccg aagtctggcg cggtgaagag
      301 gcggtttcgc tctctgctac agaatataga cttttgcttc aatttgctca caatgttggc
      361 aaaatcctga catctgaaga attgttgacc agtgtttggg gtgttgaata taaaaatgat
      421 aaggaaattt tatgggtgag cattgcccgt ttacgccaga aacttgaaaa agatgctcac
      481 acccccaggc acattgttac ccgttctggg ctagggtatc tgatgccccc tattgaaacg
      541 taaaacttgc gcataaaacg ggttgcgctc aggaacaaga tatgtctcaa aagaaaattt
      601 tgatcgtaga ctcagatgtc gcaagccgaa actttattgc acgaaaatta caagaacttg
      661 attatgaaac cttacaggca gggtctggta aggaaggtct gatttataca tggcgcgacc
      721 atccagacct tgtgattgct gaccctgctt tgaccgatat caagggggaa gagtttgcta
      781 ctaaactacg caacgacccg cgaactgcta atttgcctat tgtcgcttta agtagcgacc
      841 ataaccctgc tcgtatcaaa tcttgccagg atgcgggctt caacgattac attacgaaat
      901 cgggacaagc ggtaccgatg ttgaaagaca tcatcaatcg cttgtttggc gttacacaag
      961 aagatatgaa acagggtggt ttgatgatcg tttttctcag tgccaaaggt ggcacaggta
     1021 cttcttcagt ttgtgccaat acagcggtca atattacgaa ggatcaaccc gaagcacgaa
     1081 ttgccgtggt ggatttggta tttcctatcg ggtccattgc tcagattgta ggctacgaag
     1141 ggactgataa tattgtcact gttgcagacc gaaacccatt ggaaactgat gtggattttc
     1201 ttaagacttt gcccagaatc gagccttgga atttcaacct cttggcaggt tctccagacc
     1261 ctgaactgag taaccaactg aatgtggggc gcatttggga tattattact gggctgaaat
     1321 cttcctatga ttatgtccta attgacattg ggcgctctct ctcaaagatt acactgcctt
     1381 taattcaaca tgctgacttg atttctttgt taattagcac agatgtcagc accatctcgt
     1441 tgaccaaaac catattggat tttctcaaat cgaaaggggt aaagccagaa gcaatctata
     1501 ccatcttaaa tcgccatgct agtttggaag gtctaagcaa acgagaagct gaaacgattt
     1561 tggagaaaaa tgtaaatact gcaattccct acctcggtac aaatttcgga tttgcaaaca
     1621 gccaacaccg cccttttaca ttaaaattcc ctaacgatac agcctctata atttttcatg
     1681 attctgccag ggaaatggca aacctcgcaa aaaagatccg tagccaatag gtgtctttcc
     1741 cgggcagagg gagataaagc ccatgtctat agaatatgac gataaaggaa agtactacac
     1801 aaatgttatt caaaaagtgc ctgtgccagc catggtacag accacaacaa atctcatccg
     1861 cggaatagtt catgtacggc atggtgaacg tttgaaggat gagatagaag gcagcgagca
     1921 atttgttgcc atgacggatg ccagtgttta tagcgcggat gggaaagagc tgtacacagg
     1981 tccattcttg gctctacaaa aagggcagat cgtttgggtc atgccgttga atgagacaca
     2041 aaaaggggaa gaatgaacac accactttca ggcgctccac aacggttaac tgggcaggac
     2101 cttattcgcc tgaagaagta tctggcagat aatcttttac gtgcactcga aacagaaggg
     2161 atcgaagcat cgcagcgagt gacatatatt cagcaaaaca tcgggcgtgt atttgatact
     2221 acacagctca aattccctga agacctaaaa cgagaaattt ttaagcaggt tttgaatgac
     2281 ctgttgggct ttggtcccat ccaaccactt ttggatgacc cagatatttc tgagatcatg
     2341 gtgaatgggc ctaaaaagat tttcatcgag aaaaaggggc agcttaccaa gagcgagatc
     2401 acctttgatg atgacgatca tgtattgcgc gttattgacc gaatcattat gccattgggt
     2461 cgccgcgtgg attttgactc acctacggtc gatgcacgtt tacctgatgg ttcccgcgtc
     2521 aatgctgtag ttcgacccgt ctccattgat ggaccttcaa tcacaatccg taagtttcgc
     2581 aaagataaac tgaaggctga ggacttaatt gcatttggat ccattacaaa acaaatggca
     2641 gattttctcg aagcctgtgt gaaagcaaga tttaatattg tgatttctgg cggaacaggt
     2701 tcagggaaga ccactctgtt gaatgtggtt tctggcttta ttcccgaagg cgagcgaatt
     2761 gtgaccatag aagacgctgc tgagcttcaa ctgcaacaag accatgtcat gcgtatggag
     2821 acgaaacccg ccaactccga cgggttgcac gcagtgacta tccgcgacct tgttaagaac
     2881 tctttgcgta tgcgcccaga ccgtatcgta gtgggggaag tccgtggtgg ggaagcgctt
     2941 gatatgcttc aggctatgaa cacggggcac gatggttcat tgaccactgt ccattcgaat
     3001 agcccgcgtg atgcaatttc ccgtctagag acactggttt taatggcggg tatggaattg
     3061 ccgcttaagg tcgttcgcac ccagatttca tcggcaattg accttattgt tcagcagacg
     3121 cgtcttaagg acggcaaacg taaggtaaca gcaatcactg aagtcgctgg tatggaaggc
     3181 gatgtggtcg ttctaactga tattttcaaa tttaaccaaa ccggtgtaac gcaagacggt
     3241 caagttttgg gcgagttaca gcctacgggt attcgcccca acttca
//
LOCUS       DAOREF010000278         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18962_length_3272_cov_4.149829, whole genome shotgun sequence.
ACCESSION   DAOREF010000278 DAOREF010000000
VERSION     DAOREF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18962_length_3272_cov_4.149829"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /gene="rsfS"
                     /locus_tag="PKI33_06950"
     CDS             complement(<1..260)
                     /gene="rsfS"
                     /locus_tag="PKI33_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNM36780.1"
                     /translation="MDIKEIASFTDYFVICTGTSDRMLDALANSAIDEVRKKHKKKAK
                     KQGNSRDGWVVVDFGDVVLHLLSPDQREYYQIEDLWEAGKIL"
     gene            complement(336..914)
                     /gene="thpR"
                     /locus_tag="PKI33_06955"
     CDS             complement(336..914)
                     /gene="thpR"
                     /locus_tag="PKI33_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNM36781.1"
                     /translation="MNQIRTFIALDFPLSILESIEQQTQRLRQTLGEDFIRWVPTHNL
                     HLTLKFLGNIPISHLEFIKQMLTQAADSSSQFDLQISGFGSFPNSKRPRVLWVGVHEP
                     ATLATLQKAVEEGAHHLGYEKEERPFSPHLTLGRVRQGINVTELQKISNTISSFQLGK
                     IGTARVDSVHLYKSDLNSEGSVYTKLFSTPLR"
     gene            complement(917..1300)
                     /locus_tag="PKI33_06960"
     CDS             complement(917..1300)
                     /locus_tag="PKI33_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36782.1"
                     /translation="MNSRQKTVMIIEDEADAAELFSEMMRINGFRVIKMFSSAPAIPI
                     ISQEKPDIILLDVMMPDISGLEVLRYIRREPELASIPVIILSAKSMPGDIKTGLEAGA
                     SMYLTKPVGFQDLKNAVEKVLQASS"
     gene            1430..1936
                     /locus_tag="PKI33_06965"
     CDS             1430..1936
                     /locus_tag="PKI33_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNM36783.1"
                     /translation="MSELELFRAEKNEFFGSHPQSPLTREQKKDFHGLNYFPENETLR
                     LEVKVEEFSVKDELVMQTTTGGVQHYERFGKFSFDVESQTVELTVYRSEHGFFLPFVD
                     ALAGSETYPAGRYLEPDPLPGGRFFIDFNLAYNPYCAYNEMWSCPITPAENRLKVPIR
                     AGEKLFHE"
     gene            complement(1967..2263)
                     /locus_tag="PKI33_06970"
     CDS             complement(1967..2263)
                     /locus_tag="PKI33_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36784.1"
                     /translation="MNNEHLPTPQNKPSQPEITDEVALRFLKILEQVREEDLSCSDMY
                     ARLDEFVEAEIKDGKDAAILTPLIHEHLDMCSGCCDEYEALLNVVENTSNTQDE"
     gene            complement(2331..2969)
                     /locus_tag="PKI33_06975"
     CDS             complement(2331..2969)
                     /locus_tag="PKI33_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNM36785.1"
                     /translation="MSSRTNETWLSDLRSSGPVHEEALSDLRAIIQKGLPYALSRWLS
                     SDDPLFPPLVEEVTQETLLRVLDQLDTFEGRSLFTTWVHKIAIRIALTELRRKRWRDA
                     SLDELTENEDAPPPPGLLADPHASPETSAERKDMVSRVRRILEEELTPKQREALVLLG
                     LQDMPMEDAAKRMKTNRNALYKLLHDARLRLKKRLALEELTPQDVLLAFEQK"
     gene            complement(3088..3161)
                     /locus_tag="PKI33_06980"
     tRNA            complement(3088..3161)
                     /locus_tag="PKI33_06980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3125..3127),aa:Val,seq:cac)
BASE COUNT          844 a          684 c          825 g          919 t
ORIGIN      
        1 aacagaattt tcccggcttc ccataagtct tcgatttggt agtattcgcg ttggtctgga
       61 gaaagaaggt gcaagaccac atctccaaag tcaacgacca cccagccatc acgggaattg
      121 ccttgttttt tggctttttt cttatgtttt ttacggactt catcaatggc gctgttagca
      181 agcgcatcta acatgcggtc actggtgccg gtacagatga cgaaataatc agtaaaagaa
      241 gcaatttctt tgatatccaa caggacgatg tcttcgcctt ttttatcttc gagagcatct
      301 acaatattgc gagcaatttc tagggtattc agagttcacc tcaatggggt tgaaaataac
      361 ttcgtataga ccgaaccttc tgagttcaag tcacttttat aaagatgcac agagtctact
      421 ctggctgtgc caattttgcc aagttgaaat gaggaaatcg tattactgat cttctgtaat
      481 tctgtgacgt taattccttg acggacacgc cctaatgtaa ggtgcggcga gaaggggcgt
      541 tcttcttttt cataaccaag gtgatgagcg ccttcttcga cggctttttg gagtgtggca
      601 agagtggctg gttcgtggac tccgacccag aggacgcgag gtcgctttga gtttgggaaa
      661 gagccgaagc cgcttatttg taaatcgaac tgcgacgatg aatcagctgc ttgggtgagc
      721 atctgtttga tgaattccag atgcgagatg gggatgttgc ctaggaattt taacgtcaaa
      781 tgcagattat gagttggaac ccaacggatg aaatcctccc ccaaggtttg gcgcaggcgc
      841 tgtgtttgct gttctattga ttcgagaatg gataatggaa aatccagagc aataaaggtt
      901 cggatctgat tcattcctag cttgaggctt gcagtacctt ctcgacagca tttttgaggt
      961 cttgaaagcc aacaggtttg gtgaggtaca ttgaagcccc agcttcaagc ccggttttga
     1021 tatcgccggg catgcttttg gctgaaagga taatgacagg aatggaggcg agttcaggtt
     1081 cgcggcgaat gtaacgtaac acttcaagac ctgaaatatc gggcatcatt acatcgagca
     1141 aaataatgtc tggcttttct tgtgagatga tgggaatggc gggagcgctg gagaacattt
     1201 tgataacacg gaacccattg atgcgcatca tttctgaaaa aagttcagcg gcatcggctt
     1261 cgtcttcgat gatcatgacc gttttttgtc tcgaattcat tttgtttcct ctactatgct
     1321 aaaaatagca taaaggtatg gaatttgcaa gggcgcatga tttttgcgat gtccttgtaa
     1381 gaattcggat ttgttccatg tctatgcaaa ataagatttg gaggtatcaa tgagtgaatt
     1441 ggagcttttt cgtgcagaaa agaatgagtt ttttggcagc catccgcaaa gcccgctaac
     1501 ccgtgagcag aaaaaagact ttcatgggtt gaattatttt ccagaaaatg aaactttacg
     1561 gctggaagtg aaagtggaag aattttcagt taaggatgag ttggtgatgc aaaccaccac
     1621 aggtggtgtt cagcactatg aacgctttgg aaaattctct tttgatgtgg agagtcaaac
     1681 cgtagagttg accgtttatc gtagcgagca tggctttttt ctgccgttcg tggatgcgtt
     1741 ggctggtagt gagacgtacc ccgccgggcg ttaccttgag ccagaccctt tgccaggtgg
     1801 gcgcttcttt attgacttca acttggcata caacccatac tgtgcctaca atgaaatgtg
     1861 gtcatgccca atcaccccgg ctgagaatcg cttgaaggtg cccatacggg ctggtgagaa
     1921 actattccat gaataaaaaa aatgacgggc tagcccgtca ttttttttat tcatcttgag
     1981 tattgcttgt attttctaca acattcagta aagcttcata ttcgtcacaa cagccggaac
     2041 acatatctaa atgctcatgg atgagtggag tgagaatggc ggcgtcttta ccgtctttga
     2101 tctcggcttc cacaaattca tccagccgcg catacatgtc actgcaagag agatcctctt
     2161 cacgaacttg ctcaaggatc ttcaagaatc gcaaggcaac ttcatccgta atctcaggtt
     2221 gagaaggctt gttttgaggg gtagggagat gttcgttatt cattctaaag ggtgagacga
     2281 ttaataaaaa cttaatctta ctttgtcaat tatacccaat tgataagaaa ttatttctgc
     2341 tcgaaggcga gtagaacatc ttgcggtgtc agttcttcca gcgctaggcg ttttttgagg
     2401 cgcaggcggg catcgtgaag cagtttgtat aatgcattgc ggttggtttt catgcgtttt
     2461 gcggcatctt ccatgggcat gtcttgcaag ccaagcaaaa ctagcgcctc tcgctgtttt
     2521 ggggtcagct cttcttccaa gatgcggcgt acacgagaga ccatatcctt gcgttcggca
     2581 gaggtttcag gggatgcatg agggtctgca agcagaccgg gaggaggagg agcgtcttca
     2641 ttttccgtta gctcatcgag agacgcgtca cgccagcgct tgcggcgaag ctcggtcaat
     2701 gcgatacgga tggcaatttt gtgtacccag gtggtgaaaa ggctacgccc ttcaaaggta
     2761 tccaattggt ctaatacgcg taggagggtt tcctgggtta cttcttcgac caggggagga
     2821 aagagaggat catctgacga tagccagcgt gatagtgcgt acggcaaacc cttttggatg
     2881 attgcacgca agtcgcttaa cgcctcttca tggacgggac cagaactgcg taaatcactt
     2941 aaccaagttt cattagtgcg agaagacata acgtagtcat tataagatac tgcaaggggt
     3001 ttttcagcat ccctgagaga tactgatagt atttttataa atccgtttcg gaaaagaaaa
     3061 aaaccgcctc aaggagacgg ttttctgtgg gcgcgcaggg actcgaaccc cggaccccta
     3121 ctgtgtgata gtagtgctct aaccaactga gctacgcacc caacggagcg agattatagc
     3181 cgagcctctc gtcttttgca agcctaatgt gccaggtctt cgggtctaaa acaggcttca
     3241 taatctgcac caatgatgac gcggtattga ac
//
LOCUS       DAOREF010000279         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_18970_length_3272_cov_2.642835, whole genome shotgun sequence.
ACCESSION   DAOREF010000279 DAOREF010000000
VERSION     DAOREF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18970_length_3272_cov_2.642835"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKI33_06985"
     CDS             <1..1195
                     /locus_tag="PKI33_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36786.1"
                     /translation="NVLIAFTFGMLSLLLYHRYFQNSGNKKLFLLFGSAICFSLTIFS
                     AEAGIAIVGYLLAYCWFLLKSSKWKWLTLIPHLFVFIIWITFYRMGNYGGWGTSYIDP
                     VRELIPFLLAAFERLPVLTLGFFLYPPAELYPFVTDIWLKYLWVSAGFGLTVFLGLKI
                     YLTIRKNQNLKFFLVGAVFSLLLIGSSLPANRLLFFVGMGSFAALASFLMDGNVQTWV
                     KMPLYVVHLCLAAFLLPIMSYSPRLFGEIEPAILSAPIKPTVVIVSAPSAFHADFFKL
                     IRLRYEIEAPERVWNLGASLSSMTIYRQSENTLVINASNGYISGFDTVFRGNAHPMFK
                     GQTVNLNGLQVTVQAVTPDGRPAMVRFEFAEPLQSAEYQWLIWKSNRLIEWNVPEIGQ
                     SVHVP"
     gene            complement(1200..2105)
                     /locus_tag="PKI33_06990"
     CDS             complement(1200..2105)
                     /locus_tag="PKI33_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNM36787.1"
                     /translation="MSKARKLIPYFIVFDILVLIAVIVALNFDVVLRLNNAYKAGTLT
                     EQKIRYEIAAYDLNDWRRVEPIDQKTSPSDGMVQVYVPEGEFIMGKPGEPDFDSPEHL
                     VYVNAFWIDRVEISNQLYAACVEAGGCSQPFIDENPFYGRWVYRNLPVTFVNWYQATE
                     YCEWAGRRLPTEAEWEKAARGTDQRPYPWGEADATPRLANYAESLIGEPVSVYRYPSG
                     ASPYGALNMVGNVREWLADWFSTDYYLSSPSNNPTGPETGIERSMRSGAYDADTNEIY
                     TTSRYKHEPQSAGLSRGFRCAETAK"
     gene            complement(2135..>3272)
                     /locus_tag="PKI33_06995"
     CDS             complement(2135..>3272)
                     /locus_tag="PKI33_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNM36788.1"
                     /translation="CSFLSFQPDLNWRNPEVKQTMFNIARFWLEKGVDGFRLDIFNTI
                     YKDAEFRNNPFTFKLAPTPDDPSGYFQKAQYSQDQPESFELAKELRQVTNEFGERLTV
                     GEITAKRNIVRKFAGEEKNDGLTLAFDFEMLEFTFTAEYFRKLIGNIETHFHPPFMPV
                     YVFSNLDKRRSIHRLGDDVRKAKLLHMLQLTVRGVPCMYYGEEIGISNLKLPFNTALD
                     PIPHKYTFAPRFIFDMLDITVNRDEVRTPMQWDTTKNAGFSSAEKTWLPVHPNHSLVN
                     VESESKDEHSLLNTIKSLMKFRNENSAIKEGDFEFIDGLPAGVLGYKRSLNGQSFLVL
                     LNFDETAKDVRLRFQNAAFRLTVKDEAQGDIAHLNSFGGLILFV"
BASE COUNT          815 a          741 c          740 g          976 t
ORIGIN      
        1 taatgtattg attgcattta cctttggcat gctttccctt ttgctttatc atcgctattt
       61 tcaaaattca gggaacaaaa aactgttttt attgttcggt agtgctatct gcttttcgtt
      121 gacaatattt tctgctgagg caggtatcgc cattgttggc tatttattag cgtattgctg
      181 gtttctactc aagtcttcaa aatggaaatg gcttacctta atcccgcatc tgtttgtttt
      241 tataatatgg ataacttttt acagaatggg gaactatggc ggatggggga cttcttacat
      301 tgaccccgtc cgcgaattga tccccttttt gttggctgct ttcgaacgtc tgccagtatt
      361 aacactgggc tttttcttat atcctccggc agagctttat ccctttgtaa ccgatatatg
      421 gctcaaatat ctttgggtga gcgctggatt tgggctgaca gtctttttgg gtctgaaaat
      481 ttacctgact attcgaaaaa atcagaattt gaaattcttt cttgtaggag ccgttttttc
      541 tctgcttctt attggctcat cactgcctgc aaatcgcttg cttttctttg tagggatggg
      601 cagttttgct gcactggctt cttttctgat ggatggaaac gtgcaaactt gggtcaagat
      661 gcctttgtac gttgtgcatc tatgtctagc ggctttttta ttgccgatta tgtcatatag
      721 ccccagatta tttggagaga ttgaaccagc tattctatcg gcaccaatca aacctactgt
      781 cgttattgtt tctgctccca gcgccttcca cgcagacttt ttcaaactca tccgcttgcg
      841 ttatgagata gaggcacctg aacgcgtatg gaatttggga gcaagtctct cgtctatgac
      901 gatttatcgc caaagtgaaa acaccttagt gattaacgct tctaatggtt atatttcagg
      961 gtttgatact gttttccgtg gcaatgctca tcctatgttt aaggggcaga ctgtaaactt
     1021 aaacggcttg caagtcaccg tgcaagccgt cacaccagat ggacgccctg caatggtaag
     1081 gtttgaattt gccgagccat tgcagagcgc tgagtatcag tggttgatct ggaaatcaaa
     1141 tcgccttatc gaatggaatg tccctgagat agggcaatct gttcacgttc cgtgaaatac
     1201 tattttgctg tctccgcaca gcggaacccg cggcttaatc ctgcagactg tggctcatgt
     1261 ttatagcgtg atgtagtgta aatttcatta gtgtccgcat cataagcgcc actgcgcatt
     1321 gaacgttcaa tgccagtctc tggaccggtt gggttgtttg atggcgaact gagatagtaa
     1381 tcagtgctga accaatctgc taaccattcg cgcacgttgc caaccatgtt gagagcgcca
     1441 taagggctgg ctccgcttgg gtaacgatac acagaaacgg gttcaccgat caaagactca
     1501 gcgtagttcg cgaggcgcgg agttgcatcc gcttcacccc aagggtatgg acgttgatcg
     1561 gtgccacgtg ctgctttttc ccattctgct tcagtaggca aacggcgtcc agcccattcg
     1621 cagtattcag tagcttgata ccagtttaca aaagttactg gcaaattgcg atagacccat
     1681 ctgccataaa atggattttc atcaataaac ggttgcgaac aaccaccagc ttccacacaa
     1741 gcagcataca gttggttaga tatttctacg cggtcgatcc aaaaagcatt cacatagacg
     1801 agatgctctg gcgagtcaaa atccggttcg ccgggcttgc ccataataaa ttcaccttca
     1861 ggcacataca cttgcaccat accgtcacta ggcgaagtct tttggtcaat aggctcaaca
     1921 cgccgccaat cgttcaagtc atatgctgca atctcgtagc ggatcttttg ttccgtcagc
     1981 gtgcctgctt tgtatgcgtt attcagacgc agcactacat caaaattgag tgcaacaatt
     2041 actgcaatca acacaaggat atcaaaaaca atgaagtaag gtatcagttt tcgggcttta
     2101 gacatgggcg acattatacc tagcacagat gcctttatac aaaaagaatc agcccgccga
     2161 aactgttcaa gtgcgcaatg tcgccctgcg cttcatcctt aactgtgagc ctgaatgcgg
     2221 cattctggaa ccttaagcgc acatcctttg ctgtctcgtc gaagttcaag agcacgagga
     2281 aagattgtcc gttcagagag cgtttgtatc cgagcacgcc tgctggcaat ccatcaatga
     2341 attcaaaatc tccttctttg atggctgaat tctcattcct gaatttcatc aaagatttga
     2401 tggtgttcaa taaggagtgt tcatctttac tttcactttc cacattcaca aggctgtgat
     2461 ttgggtgaac aggcagccac gttttttcag cggacgaaaa ccctgcgttc tttgtggtat
     2521 cccattgcat gggtgtacgg acctcgtcgc ggttcacggt aatatcgagc atgtcgaaga
     2581 taaagcgtgg ggcaaaagta tatttatgcg gaatgggatc aagcgccgtg ttgaatggta
     2641 atttcaaatt gcttataccg atctcttcgc cataatacat gcaaggcaca ccgcgcacag
     2701 ttagttgcaa catgtgtagc agcttcgcct tgcgcacgtc atctccgagg cggtgaatgc
     2761 tacggcgctt gtcgagattg ctaaatacat agaccggcat aaagggcgga tgaaaatgcg
     2821 tttcaatatt gccaatcaac ttgcggaagt attcggctgt aaatgtgaat tcgagcatct
     2881 caaaatcgaa ggcaagggtc agcccatcat tcttttcttc tcccgcaaac ttgcgcacga
     2941 tattccgttt tgccgtaatt tcaccgaccg tgaggcgttc accaaattcg ttggtaactt
     3001 gtctcagttc ctttgcaagt tcaaaacttt caggttgatc ttgtgagtat tgtgctttct
     3061 gaaaataccc agaggggtca tcgggggtgg gtgcaagttt gaatgtgaaa ggattatttc
     3121 gaaactcggc atctttgtaa atcgtgttga aaatatccaa acggaagcca tccacacctt
     3181 tttcgagcca aaaccgggca atgttgaaca tggtttgctt gacttcggga ttgcgccagt
     3241 ttaaatcagg ttgaaaggac aggaaactac ac
//
LOCUS       DAOREF010000280         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19044_length_3264_cov_4.721409, whole genome shotgun sequence.
ACCESSION   DAOREF010000280 DAOREF010000000
VERSION     DAOREF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19044_length_3264_cov_4.721409"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PKI33_07000"
     CDS             <1..586
                     /locus_tag="PKI33_07000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNM36789.1"
                     /translation="MIRTALESTPGGSEIPASVVVLSQSLLALTLAPFLNMLFTMGEE
                     LGWRGFLLPRLLPLGQWKAVLWSGLIWGLWHAPVIVQGHNYPGYPILGVFMMIVFCFL
                     LGTIIAWLYINTKSPWVAALAHGAVNAIAGLPVLFLAPGFNMAFGGTLAAPTAWIGMA
                     LFIAWLVWTKRFPVQEQTGDAQSTSEVDRSENTK"
     gene            complement(643..3018)
                     /locus_tag="PKI33_07005"
     CDS             complement(643..3018)
                     /locus_tag="PKI33_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNM36790.1"
                     /translation="MTLPRVSIDNVLRILLSLSQYPILSSRIRHQMRKTLYSRGIVRK
                     EVFEEEVKRKAVESQTLEGITDPLTEESNEVWLMRITRVKDSLTDFYFAYNLPYAEFE
                     DLVRKVLAERGSIDSDFVWVNPELAPQELLFEQAEMIESMPDADKEKYEARLQAIIAV
                     LIRTMISDQLKYIKIARQWFTVEDLREINRRKIGGGKIGGKAAGMLLAMRILKETAPV
                     QIRNRFRLPISYYLGSDVYYNFMSLNDLGGWNGQKYKTEEQMRADYPELCEDFYKGQF
                     PPEVIESLERVLEEAGQRPLIVRSSSLLEDNFGTSFAGKYESIFLPNQGDANECLKAL
                     TQAIAKIYASVMNPDALIYRHTKELADYDERIGILIQIVEGERAGRYYFPHGAGVAFS
                     RNLFRWSPQIKQDEGFLRLVTGLGTRAVDMLADDYPRLVALSHPRLHSSSDVRTIKRY
                     SQQHLDAIDIETNSFVTIPVRDALHTDYDPLRYIAQVEQDGYLAPIRSRLDSTDKLVI
                     TFDGLLSRTPFSKSMRAALTLLETHYGSPVDTEFTVEIVNPDSQPDVRITILQCRPQS
                     HIHEANEVQIPSDLETQDIVFSSQTMVPQGVVENIGYVLFVPSEGYFSLETQAERTQL
                     ERAIGQLNAALKGQTFIAVGPGRWGTSTPDLGVHVAYSDIYNSRALVELAGEEIGASP
                     EPSFGTHFFQDLMEANIYPLGVFVDEEGTIFKRDFFYSTPNRLSEFISIENPRVVAAL
                     KLIAVSDFRPNTHMNLIMEASKSRAVAFLVGKATPIESDDAIVGTAPTSLE"
BASE COUNT          867 a          741 c          774 g          882 t
ORIGIN      
        1 catgatacga actgcattag agtctacgcc gggtggaagt gaaattccag caagcgttgt
       61 tgtcttatct caatcattgc tggcattaac acttgcaccg tttttaaaca tgctattcac
      121 gatgggagaa gaattaggct ggcgtggatt tttacttccg cgattgttgc cactcggaca
      181 atggaaagcc gttttatgga gtggcttgat atggggcttg tggcatgcac ctgtgatcgt
      241 acaaggacat aactatcctg gctatccaat actgggtgtg ttcatgatga tcgtattttg
      301 cttcttgctt gggacgatca ttgcatggtt gtatatcaat accaaaagcc catgggttgc
      361 agcgcttgca catggagcag tcaatgcaat tgcagggttg cctgtattgt tcctggcacc
      421 tggtttcaat atggcattcg gtggaacact tgcagcacca actgcatgga ttggcatggc
      481 tttgttcatt gcatggttgg tgtggacgaa acgtttccca gtacaagaac aaacaggtga
      541 tgcacaatcg acatcagaag ttgataggag tgaaaataca aaatagaaca tttgttcttt
      601 ttgtaattat aaaaaatggt cgacgatgag tcgcccattt ttttattcca aacttgtcgg
      661 tgctgttcca acaattgcgt catcactttc aattggagta gcttttccaa ctagaaaagc
      721 gacagctcta cttttgcttg cttccataat caaattcatg tgagtatttg gacgaaagtc
      781 tgaaacagca attaatttca aagcagcgac aactcgtgga ttttcaatag agataaattc
      841 agaaagtcga tttggggtgc tatagaaaaa gtcacgcttg aaaatagttc cttcttcatc
      901 aacaaataca ccaagcggat agatattcgc ctccataagg tcttggaaga aatgggtgcc
      961 gaaagaaggt tcaggagatg ctccgatctc ttctcccgcc aattcaacca aggcacgtga
     1021 attatagata tcgctgtacg ccacatgcac acccaaatcc ggtgtcgatg ttccccagcg
     1081 tccaggtcct actgcgataa aggtttgtcc tttcaacgcc gcattgagtt gaccaatggc
     1141 gcgttccagt tgggtacgct ctgcttgtgt ttcaaggctg aaataacctt ctgaaggaac
     1201 aaacaaaaca tacccgatat tttccacaac cccttgtggc accatggttt gtgacgagaa
     1261 aacgatgtct tgtgtttcaa ggtcacttgg aatctgtact tcattcgctt cgtgaatatg
     1321 actttgcgga cgacactgca aaatcgtaat gcggacatct ggctggctat cagggtttac
     1381 tatttcaacg gtgaactctg tatcgacagg cgaaccatag tgtgtttcaa gcaaagtcaa
     1441 tgccgctcgc atggactttg agaaaggcgt ccgcgagagt aagccgtcga aggtaataac
     1501 cagtttatcg gtagagtcga ggcgcgaacg gattggagcg aggtatccgt cttgttccac
     1561 ttgggcgata taacgcagcg gatcataatc cgtgtggaga gcatcgcgca cggggatggt
     1621 tacaaaagaa ttcgtttcaa tgtcaattgc atcaaggtgc tgttgcgaat agcgtttgat
     1681 ggtgcggaca tctgaagacg aatgcaaacg cggatgactg agcgctacta atcgcggata
     1741 atcatctgca agcatatcga cggcgcgggt acctaaacca gtaacaagac gcaagaagcc
     1801 ttcatcctgt ttgatctgcg gagaccagcg gaaaaggttt cggctaaatg ccacaccagc
     1861 cccatgggga aagtaatatc taccagctcg ttcgccttca actatttgaa tcagaatacc
     1921 gatgcgttca tcataatcag caagttcttt ggtgtgacga tagatcagcg catcggggtt
     1981 cattacactg gcatagatct tggcaatagc ttgcgtgagt gccttgaggc attcattagc
     2041 atccccttga ttgggcaaga aaatgctttc gtattttccg gcaaacgatg tgccaaaatt
     2101 atcttcaagc aaactggagg aacgcacgat caacgggcgt tgacctgcct cttctaaaac
     2161 gcgctcaagg ctttcgatca cttcaggtgg aaattgtccc ttgtaaaaat cttcgcacag
     2221 ctcggggtaa tctgcacgca tctgttcttc ggttttatat ttctgcccat tccaaccacc
     2281 aaggtcattg agcgacatga agttgtagta cacatcggag ccaagataat aagaaatggg
     2341 caaacggaaa cggttgcgga tctgtaccgg cgcggtctct tttaggatgc gcatggcaag
     2401 caacatgccc gccgctttac cgccgatctt tccgccgccg atcttgcgac gattgatctc
     2461 gcgcaggtct tccacagtaa accactgacg cgcgatcttg atgtatttca attgatcgct
     2521 gatcatagta cggatcaaca ccgcaataat ggcttgcaga cgcgcttcgt acttttcctt
     2581 gtctgcatct ggcatggatt cgatcatctc tgcctgttca aaaagcaatt cctgtggcgc
     2641 tagttctggg ttgacccaaa caaaatctga gtcaatactg ccgcgttctg ccaacacctt
     2701 gcggactaaa tcttcaaatt cagcatacgg caggttgtat gcaaaataaa aatcggtgag
     2761 cgagtctttt acacgggtga tacgcatcaa ccaaacttca ttcgactctt ccgtcaacgg
     2821 gtcggtaatg ccctcaagcg tctgagattc aacggctttg cgttttactt cttcttcaaa
     2881 aacctctttt cgaacaatac cgcgtgaata cagcgtcttc cgcatttgat ggcgaatgcg
     2941 actactgaga atgggatatt ggctcaacga aagcaagatg cgaagtacgt tatcaatgga
     3001 aacgcgtggg agagtcatga attcaccttt ggtggacaat tgacgataga gacaaaacgc
     3061 aggtcgatca tctgcggttc attgtccgat tttataacaa aaaatccgcc agataggtct
     3121 ctggcggatt cgtattctgt ttgtaagcct atcccaaatg catcggcata atgacgtgca
     3181 agaagttgtc atcaccaact gggcggatga ctcccggcgc attctgagcc gtggtctcta
     3241 gtgccacatt cggggtcttg atga
//
LOCUS       DAOREF010000281         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19103_length_3259_cov_4.131398, whole genome shotgun sequence.
ACCESSION   DAOREF010000281 DAOREF010000000
VERSION     DAOREF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19103_length_3259_cov_4.131398"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKI33_07010"
     CDS             <1..624
                     /locus_tag="PKI33_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNM36791.1"
                     /translation="RALKYIEEHVMGFTRSSEEVPGYEIPWMYFDYLRTGDARPLGGV
                     FYHNAMDVVAMAALLAHVSEILADPYNGRVEHGLDFIALGKLFEDLGHWDEAARLFER
                     GLEIGLDESDFGVAVKRISILQKKRGDMDRAVELWKAAAKKGHIYAHIELAKYYEHKM
                     RDAKAAVKWAKSARAEAEKADLPPYIRNHWFHEIDHRLERLTRKAGL"
     gene            660..1073
                     /locus_tag="PKI33_07015"
     CDS             660..1073
                     /locus_tag="PKI33_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM36792.1"
                     /translation="MEINISQEKGNVPVTVIQVTGQLDGQTYQKLIAAAQEVIKEGST
                     NILLDLSELTFISSAGLVSLHVIALLLRGEAMPDLDHGWAALKSVNKSRESGVQQHVK
                     LLNPREEVINVLDMVGFSSFFEVFKDKQKALESFS"
     gene            1141..2418
                     /locus_tag="PKI33_07020"
     CDS             1141..2418
                     /locus_tag="PKI33_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNM36793.1"
                     /translation="MKRNLGNIVFTILVLLTIVVWVVFPPHSEGVENYLRYHIGMTMG
                     SVVIVLMSFSLFISTRPRWAEPYFGGLDKMYMTHRYTSTAAFLLMFVHLLIVPLHLVN
                     LALGNYLAIVAFLGFIAIVLPTLAPRIPFLNKLTSGTYEGWKRLHGFIGIFFILGYLH
                     SITVNAPTTKIAINWNQIFVFLGIGSYIYTEVLSRFLGKHVPYTVKAVNHPNNSTTEV
                     VMQAKGKPIQHAHAGQFLFVRFKGDKILDEAHPFTISSAPHEGELRVTVKAVGDFTRY
                     LFQNLKPGMDAIVEGAYGMFNYKTGSDKQIWVAGGIGITPFLSFLRDLKTDLAHDVHF
                     YYTVRHPEEAVFADEIEAIAKKHPRLKAHIRFSSQHGSLTVDEIVKNAGGDASKHHVY
                     MCGPLPMVQAFASKFVEAGVSKDNVHFEEFNFR"
     gene            2434..2841
                     /locus_tag="PKI33_07025"
     CDS             2434..2841
                     /locus_tag="PKI33_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM36794.1"
                     /translation="MQLSFSKQDNVTVLHVMGDVDSSNYTEVIIKAQQLYDDGTRNLL
                     LDLANVPYVSSAGLMAFHTIARVFAGHSIQGGESGRPTFRSINPKDDASAREHVKLLS
                     PQPAVEQVLDVIGLKQFFEIYTDLKVAVQSFTS"
     gene            2974..>3259
                     /locus_tag="PKI33_07030"
     CDS             2974..>3259
                     /locus_tag="PKI33_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNM36795.1"
                     /translation="MSKKKSTRKTSSMAQGGLSSFLRKPTGQVTVIAVLALVIYLIAS
                     SVRGGSSAGLASTVSADEAYQMYQNGTFVLDVRTQAEWDDYHVPNTTLIPL"
BASE COUNT          823 a          701 c          825 g          910 t
ORIGIN      
        1 cgtgcattga aatatatcga agaacatgta atggggttta cgcgttcttc tgaagaagta
       61 cccggttatg aaattccgtg gatgtatttc gattacttac gcacaggcga tgcccgtcca
      121 cttggaggtg tgttctatca caatgccatg gatgtggtag cgatggcggc gttgctggca
      181 cacgtcagcg agattttggc agacccctat aatggacgtg ttgagcatgg gcttgatttc
      241 attgcactgg ggaaactatt tgaagacctt ggacattggg atgaggcggc acgtctgttc
      301 gagcgtggac tcgagatcgg gttggatgaa tcagatttcg gcgtagcagt gaagcggatc
      361 tccatcttgc aaaagaagcg tggagatatg gaccgagctg tcgagttgtg gaaagcggcg
      421 gcgaagaaag gtcatattta tgctcatatt gaattggcaa aatattatga gcataagatg
      481 cgcgatgcaa aggctgcggt caagtgggcg aagtctgcac gggcagaagc cgagaaagcg
      541 gatttgcctc cttatattcg taatcattgg ttccatgaaa ttgaccatcg gttggaaagg
      601 ttgacgcgta aagcgggttt ataattgctc gaacacggat taacctttgg agaggaaaca
      661 tggaaattaa catttcacaa gaaaagggga atgtgccggt tactgtgatt caggtgacgg
      721 gtcaattaga cgggcagacg tatcagaaat tgatcgctgc tgctcaggaa gttatcaaag
      781 aaggctcaac gaatatttta ttggatctaa gtgagctaac ttttatttcc agcgcagggt
      841 tggtgtcgct gcatgtgatt gccctgctgt tgcgcggcga agccatgcca gaccttgacc
      901 atggttgggc ggcgttgaag tcggtcaaca aatcacgtga gagtggagtt caacaacacg
      961 tgaaattgct gaacccgcgc gaagaagtga tcaacgtgct cgatatggtt ggcttctctt
     1021 ctttctttga ggttttcaaa gataaacaaa aggctctcga atctttctcg taagaagaaa
     1081 cgacccacaa cgatgcaggc atgcctgcat cgttgtcatt tatattaaag gtgaattttt
     1141 atgaaacgta atcttgggaa tattgtcttt actattttgg tcttacttac catcgtcgtg
     1201 tgggttgtct ttccgccgca cagtgaaggg gttgaaaatt atctacgcta tcacatcggg
     1261 atgacgatgg ggtctgtcgt gatcgtcttg atgtcgttct cgctgttcat ttccactcgt
     1321 ccgcgctggg cagagccata ctttggtggc ttggataaga tgtacatgac ccatcgctat
     1381 acatcgactg ccgcattctt gctaatgttt gttcacttgt tgatcgtgcc attgcatctg
     1441 gtaaaccttg cgcttggaaa ttatctggca atcgttgcat ttctcggttt tatcgccatc
     1501 gttttaccta cgctcgcgcc acgcatcccg ttcttgaata aactcacgag cggaacctat
     1561 gaaggctgga agcgtttgca tggctttatt ggtattttct tcattttggg ttatctacac
     1621 tcgatcacgg tgaatgcccc aacaacaaag attgccataa actggaatca gatctttgtt
     1681 ttccttggta ttggttccta catttacaca gaagtgttga gccgctttct aggcaagcat
     1741 gtgccttata cggtgaaagc ggtaaatcat cccaataact ccaccactga ggtggtgatg
     1801 caagccaagg gcaagcccat ccaacatgct catgcgggtc agtttctgtt tgtgcgtttc
     1861 aaaggcgata aaattctgga tgaagcccat cccttcacca tttcgagtgc gccgcatgaa
     1921 ggcgaattgc gagtgacggt taaagctgtc ggtgatttca cccgttacct gtttcagaat
     1981 ctcaaacccg gcatggatgc gattgtagaa ggcgcatatg ggatgttcaa ttacaaaaca
     2041 ggtagtgaca aacagatttg ggtcgctggc ggcattggca ttaccccttt cctctcgttc
     2101 ttacgggacc tgaaaacgga tctcgctcac gatgtgcatt tttactacac ggtaagacac
     2161 cctgaagaag cagtctttgc agatgagatc gaagccattg caaagaaaca tccgcgtttg
     2221 aaggcacaca tccgcttctc ttctcagcat ggttcattga ctgtggatga gatcgtcaaa
     2281 aatgcaggtg gagatgcctc caaacatcac gtctatatgt gcggtccttt gcccatggtg
     2341 caggcgttcg cgtcaaaatt tgtagaagcg ggcgtgtcca aagataacgt ccactttgag
     2401 gaattcaatt tcagatagtc gtaggagtaa cccatgcaac tctctttttc gaaacaagat
     2461 aatgtaacgg tcttgcatgt catgggggat gtagactcgt ctaattacac ggaggtgatc
     2521 atcaaagcgc aacagcttta tgatgacggc actcgcaacc tcttgcttga ccttgccaat
     2581 gtgccgtatg tgagcagcgc cgggctgatg gcattccaca ctattgcgcg tgtctttgct
     2641 ggacattcca ttcaaggcgg ggaaagtggc agacctacct tccgttcgat caacccgaaa
     2701 gatgatgctt cagctcgaga acatgtcaag ttattaagcc cgcaacctgc tgttgagcaa
     2761 gtcctggatg tgatcggctt gaagcaattc tttgagatct atacagacct caaagttgca
     2821 gttcagtcat tcaccagtta atctccttct tttctaaatt caaacggtga cttgccaggt
     2881 tttctgacaa gtcaccgttt ctttttatat ggaagtgtaa ctttccaccg accttggcat
     2941 caaaagaata caaatctttt gatgtgaggc aaaatgtcta agaaaaaatc tactcgtaaa
     3001 acgagttcta tggcgcaagg tggattaagt agtttcttgc gtaaaccgac gggtcaagtg
     3061 actgtcattg ccgttttggc attggtcatc tatctgatcg ccagcagtgt gcgtggaggc
     3121 agttccgccg ggttggcaag cactgtcagc gcagatgaag cgtatcagat gtatcagaat
     3181 gggactttcg tactggatgt gcgcacacaa gcagagtggg atgattatca tgttcccaac
     3241 actacgctta ttccattgg
//
LOCUS       DAOREF010000282         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19188_length_3251_cov_4.493742, whole genome shotgun sequence.
ACCESSION   DAOREF010000282 DAOREF010000000
VERSION     DAOREF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19188_length_3251_cov_4.493742"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="PKI33_07035"
     CDS             complement(<1..1854)
                     /locus_tag="PKI33_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNM36796.1"
                     /translation="MNSFKLSLINDQSSTPSKEFELTKSEIIIGREDSVDITIPASAV
                     SRRHARLMLIGENYVLEDLGSSNGTYVNGEKLDKQHTLKSGDKIRLGQIISLVYTAPV
                     SEEDSNLTVVSIKDANKTAKHSGPAKPAHVMQTVLGEAPAIPQASAGPRQLIVSIAGG
                     STQTYPLTGSSISIGRLDTNDIVIPSQIVSSRHARLEQVKGGGYKLVVMPEAKNPVQF
                     EGRPIDGSHVLHHNDLLRIGSMDPGVMVTITYNAPSEASQQMERDIVFGEKTLIQIGR
                     DPSNDVVIPSPNVSRFHTQIEKVGQRYRVEDLRSSNGTYVNGERIEGSVWLKPNDSIR
                     IGQYRFVMGKDQLARYDDGNGLRVDVVGLNKWVRKDLNILQNIYASFSPREFIVVVGQ
                     SGGGKSTFVDAVAGYRPATPPSKVYVNDIDIYTHFDAIRNEIGFVPQKDIIHMELTIY
                     QALDYAAQLRMPADTSPEERHKRIMEVLEDLDLTHRKDVQISGLSGGQQKRVSIGVEL
                     LTKPGLFFLDEPTSGLDPGTETALMQLMRRLADQGRTIILITHATKNVMLADKVIFLA
                     RGGYLAWFGPPDEALDYFDQYRSERARRAGKIEFDEIYAILDDPSKGKAEDW"
     gene            2205..>3251
                     /locus_tag="PKI33_07040"
     CDS             2205..>3251
                     /locus_tag="PKI33_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HNM36797.1"
                     /translation="MMQSSSFSRKLSEENNSLVRTLRRRADLHPERLAFRFLQDDESE
                     NITINFGELDRRARAIGAWLESFNAKGERALILYPPGLDYIAAFMGCLYAGVIAVPAY
                     PPRLNRPVPRIQSIVDDSHATFALTTTSIHSNIEKRFEHAPDLKALHWLNTEQVPAGL
                     EADWRDPNVSPDALAFLQYTSGSTSQPKGVMLSHGNLMHNLEAISRGFHIDETVMGIF
                     WLPSYHDMGLIGGILEPMYLGGATTLMSPPSFLQRPVRWLEAITRYRGNTCGAPNFAY
                     DLCVDKVTSEQKETLDLSSWSLVFCGAEPIRPETLERFSRAFAGCGFRKTSFYPCYGL
                     AESSLIVSGADGPSE"
BASE COUNT          791 a          750 c          756 g          954 t
ORIGIN      
        1 ccaatcctct gctttgcctt tgctgggatc atccaaaatg gcatagatct catcaaactc
       61 gatcttgcca gcgcggcgag cccgctcaga acgatattgg tcaaaataat ccagcgcttc
      121 atcaggagga ccaaaccatg ctaaataacc tcctcttgcg agaaagatca ccttatctgc
      181 cagcataaca ttttttgtgg catgtgtaat taagatgatg gtacgtcctt ggtctgccag
      241 gcgacgcatc aattgcatta acgctgtttc tgtgccgggg tctaaccctg atgtgggttc
      301 atcaagaaag aacagaccgg gtttggtcaa aagttcaaca ccaatcgaga cgcgtttttg
      361 ctgcccgccg ctgagtccgc tgatctgaac gtctttgcgg tgggtcaggt caaggtcttc
      421 caaaacttcc ataattcgtt tgtgccgctc ttccggggaa gtgtcggctg gcatgcgcaa
      481 ttgagccgca taatcaagtg cctgatagat agtcaactcc atgtgaatga tatctttttg
      541 tggcacgaac ccaatctcat tgcggatcgc gtcaaaatga gtgtagatat caatgtcatt
      601 gacgtatact tttgaaggag gagttgctgg acggtaccca gcgactgcat ccacaaatgt
      661 ggatttgcct ccgccgctct gccctacaac aacgatgaat tctctggggc taaatgatgc
      721 atatatgttt tgaagaatat tgagatcttt gcgaacccac ttattcaaac caactacatc
      781 cactcttaag ccattcccgt catcatatct ggcaagttga tccttgccca tcacgaatcg
      841 atattgacca attcggatgg aatcgttggg cttcagccaa accgagcctt caattcgttc
      901 tccattgaca taggttccat tagaactgcg gaggtcttct acccgatagc gttgccctac
      961 tttttcgatc tgggtatgga atcgagatac attgggggag gggatgacca catcattgct
     1021 ggggtcacgc ccgatttgaa tgagcgtttt ctcgccgaag acgatgtctc gctccatttg
     1081 ttgagatgct tcactgggtg cattgtaggt tatggttacc ataaccccag ggtccatgct
     1141 gccaattctc agcagatcat tgtgatggag aacatgtgag ccgtctatcg gacgcccttc
     1201 aaattgaacc ggattttttg cttcaggcat caccaccaac ttgtacccgc cgcccttgac
     1261 ctgctcaagc cgcgcatgcc ggcttgagac aatctgagat gggatgacaa tgtcgttggt
     1321 gtcgagtctg ccaatggata tgctggaacc tgttaacggg taggtttgtg tgcttcctcc
     1381 ggcaatacta acgattagct ggcgtggacc agcacttgct tgcggtattg ctggtgcttc
     1441 accaagcaca gtttgcatca catgtgcagg tttagcagga ccggaatgtt tggcagtttt
     1501 gttcgcgtct tttatgctga caacagttag gttggaatct tcttcagaaa cgggagcggt
     1561 ataaaccagg ctgataattt gccccaatcg aattttgtca cctgatttta gggtgtgttg
     1621 cttatctagt ttttccccat tgacatatgt gccattactg cttcccagat cttccagtac
     1681 ataattttcg ccaatcaaca tcagtctggc atgacggcgt gacacagcag atgcgggtat
     1741 ggtgatatcc acactgtctt cacgaccaat gatgatttct gactttgtta attcaaattc
     1801 tttggaaggt gtactgcttt gatcattgat aaggcttagt ttgaaactgt tcacttgagt
     1861 caaaggatga tcctttcttg caaattttca acacagtttc ttgaatttat ggataagtga
     1921 gaatttgtac agaattgaga taattaagac aagattatac ctttttatta aaatgcatgt
     1981 caacctacca atgggggata cgtaaaatca ctgaaaatat tgtgtttgtt ttcacttgct
     2041 ttgctaaaaa attggtcttt tacgatggct tgaaaatttt cgtttagctt gaatgttaat
     2101 aatttcgccc tactgatggg ctatatagtt tggtgctata aatgtgttac gcttaaattt
     2161 tatgatataa attcaaaatc ttgttctttt aatggtgaaa atacatgatg caatcttctt
     2221 ctttttcccg aaagctatcc gaagaaaaca attcactggt tcgtactctg cggaggcggg
     2281 cagacttgca tcctgaaagg ctggcgttta gatttttaca ggatgatgaa tcagaaaata
     2341 tcaccatcaa ctttggggaa cttgatcgtc gggcacgtgc cattggggca tggttagaaa
     2401 gcttcaacgc aaaaggggag cgtgcactta ttctctatcc acctggattg gattacatcg
     2461 ctgcttttat gggctgctta tatgctggtg taatcgctgt ccctgcatac ccgcctcgcc
     2521 ttaaccgtcc tgtaccccgt atccaatcta tcgttgacga ttcacatgcg acctttgcgc
     2581 tgactacaac gtcgatccat tcgaacatcg aaaaacgatt tgaacatgct ccagacttga
     2641 aagcgctaca ttggttaaac accgagcagg taccggcggg gttggaagcg gattggcgcg
     2701 atcctaatgt ctcgccagat gcgctggcat tccttcaata tacatccggc tctactagtc
     2761 agccaaaagg agtcatgctt agtcatggca acctgatgca caaccttgaa gccatcagcc
     2821 gcgggtttca catcgatgag acggtgatgg gaattttctg gctgccaagt tatcacgaca
     2881 tggggctgat cggcggcatc cttgagccga tgtatttagg tggtgcaacg accttgatgt
     2941 cgccgccatc cttcttacag cgaccagtac gttggctcga agccatcacc cgttatcgtg
     3001 gcaatacctg cggtgcgcca aactttgcct atgatctgtg tgttgataag gttacatcgg
     3061 aacagaaaga aactctggat ttgagttcat ggtctcttgt gttttgcggt gcagaaccga
     3121 ttcgtcctga aacgttagaa cgcttctcac gtgcctttgc cgggtgcggc ttccgaaaga
     3181 cttctttcta cccttgttat ggattggcgg aaagttccct gatcgtctcc ggtgcggatg
     3241 gtccatctga a
//
LOCUS       DAOREF010000283         3246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19231_length_3246_cov_4.192730, whole genome shotgun sequence.
ACCESSION   DAOREF010000283 DAOREF010000000
VERSION     DAOREF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19231_length_3246_cov_4.192730"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PKI33_07045"
     CDS             complement(<1..1301)
                     /locus_tag="PKI33_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNM36798.1"
                     /translation="MANFKLTYATMFNPPEELHTGFDKAVAKLKENMGKEYGMFINGK
                     EVFADEKFEDRSPINTDWVLAKMQKGNASHAQMAVDAARKAFPMWSRTPWQQRVKLLR
                     KAASLIEDRIFELGAAMAMEVGKNRMESMGDVQETADLIYYSCYQMEKNNGYIAEMGR
                     DPLVGYESRNVSVLRPYGVWLVVSPFNFPHALTGGPSGAALAAGNTLVIKPATDTAWI
                     VRLLVDCFRDAGFPEGVVNFVTGPGSTLGQALVDSPEVDGVTFTGSFDVGMKMYRDYA
                     NRNYVRPMILELGGKNPAIVSRNADLETAAVGIVRSAFGLQGQKCSAASRVLVEEPVY
                     DELVARLKDLTDKLVLGDPTNRATYNGPVINKSSYQDFKDFTEEINQGGGKFLTGGHV
                     RVDGDYGKGFFCESTFVTDLPFEHRLWKHEMFLPITTIGKV"
     gene            1534..1821
                     /locus_tag="PKI33_07050"
     CDS             1534..1821
                     /locus_tag="PKI33_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36799.1"
                     /translation="MKPRLMFWTLGLVIALLAIACAPSNPTETAPPVVEATATVASAP
                     LVETAVPQVEATIPAVLPVATSRGPNLEATDPATVSFASGGLQLIEFFRFT"
     gene            1837..2061
                     /locus_tag="PKI33_07055"
     CDS             1837..2061
                     /locus_tag="PKI33_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36800.1"
                     /translation="MAPMVHGLEAEYFGRVKFSFLDADDPNTYNFQRELGFVYQPEFY
                     LLDANGNVLKKLVGPVSEEEFRDLFDTNLQ"
     gene            2128..3228
                     /locus_tag="PKI33_07060"
     CDS             2128..3228
                     /locus_tag="PKI33_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36801.1"
                     /translation="MDTAFHIIVFLLGAGLVIATIFSAISTFVLPRSARSQLNRLVFG
                     LTRRFIEIFMHFARTYEQRDAIMAYYAPLALMSLVPTWYMLIALGYSFMYWSLRAGDY
                     FFDLRLSGSSLFTLGFTASEGYFITILAFSEAMLGLIMVGLLIAYLPTMYSAFSRREQ
                     AVNMLEVRAGNPPNPYDMLSRFHRIHGLDKLAELWKNWEIWFADVEESHTTLPALVFF
                     RSPRSDNSWVTSAGAVLDSAAIALSSIDIPSEMSGALCIRAGFICFHRITDYFDIPHP
                     HDPKYPETPISVTRAEYDELIHKLEVAGVPIKPDREQAWKDFAGWRVNYDRTLLIMCA
                     LVMPPSNVPWSSDRAPKFKIPPLFIKKTKHQH"
BASE COUNT          732 a          827 c          800 g          887 t
ORIGIN      
        1 tgtaccttgc caatggtggt gatgggcagg aacatttcat gtttccacag gcgatgttca
       61 aaaggcaggt cggtcacgaa tgtggattcg cagaagaagc ctttgccgta gtcgccatcc
      121 acgcggacgt gaccacctgt taggaatttg ccgccgccct ggttgatctc ttcagtgaaa
      181 tctttgaaat cctgatatga actcttgttg atcacaggtc cgttataggt ggcgcggttg
      241 gttgggtcac cgagcacgag tttatcggtc aggtctttga ggcgggcaac cagttcatca
      301 tagacgggtt cttccaccaa cacgcgagac gccgccgagc atttctgtcc ctgcaagcca
      361 aatgccgagc ggacaatccc cacagccgca gtttcaaggt ctgcgttgcg ggaaacgatg
      421 gcaggattct taccgcccag ctcgaggatc attgggcgca cataattgcg gttggcataa
      481 tcacgataca tcttcatacc gacatcaaaa gaaccggtaa aggtgacgcc gtcgacttcg
      541 gggctgtcga ccagtgcctg acccagagtc gagccgggac ccgtcacaaa gttgaccaca
      601 ccttcaggga agcctgcatc acggaagcaa tctactagca agcgcacaat ccaagccgta
      661 tcagttgcag gtttgatgac aagggtgttg cccgcagcca gagcagcacc cgaaggtccg
      721 cctgtgagcg cgtgcgggaa gttgaacggt gacaccacaa gccacacacc ataagggcgt
      781 aatacagaca cgttacgaga ttcgtacccc accaacgggt cgcgtcccat ttctgcaatg
      841 tagccgttgt tcttttccat ttggtagcag gaataataaa taaggtcggc agtttcctgc
      901 acatcgccca tagattccat gcggttcttg ccaacttcca ttgccatggc ggcgccgagt
      961 tcaaagatgc ggtcttcgat caaagaagca gccttgcgga gcagttttac acgttgctgc
     1021 cagggagtgc ggctccacat cgggaaggct ttgcgcgccg catccactgc catctgagca
     1081 tggctggcgt tgcctttttg cattttggcg agcacccaat cggtgttgat gggcgagcgg
     1141 tcttcgaact tttcatcggc aaaaacttcc ttaccgttga tgaacatgcc atattccttg
     1201 cccatatttt ccttaagttt ggcaacggct ttgtcgaagc cggtgtgcaa ttcttcaggt
     1261 gggttgaaca tggttgcgta cgtgagcttg aaatttgcca taggagttct cctttagaag
     1321 tgtgagacaa atctgcaaat tatctcgtgt caccctgagc aagtgaccct attgtgagac
     1381 aaagtgcaaa ttaagtatag cgtagtattt aaccgtcgtc aaagtaaagc gactgttttg
     1441 cctgtgacat acctcatgag aagtttgtgt gagttttgta agaggatgca gtgccttggt
     1501 atcatttaat taactttaat aaggagaaaa ctgatgaaac cccgcctcat gttctggacc
     1561 ttaggcttgg taattgcgct tctggcaatt gcctgcgccc cgtccaaccc tactgaaacc
     1621 gctccgcccg ttgtagaagc gaccgccacc gtggcatccg ctccgctcgt ggaaacagcc
     1681 gtcccccagg tggaggcgac tattcctgct gttttgccgg tcgccacgtc tcgcggtccc
     1741 aacctcgaag cgacagaccc agccaccgtc agctttgcct ctggcggttt gcagctgatc
     1801 gaattcttcc gctttacctg aagtacctgc cgcgctatgg cgcccatggt tcatgggctt
     1861 gaagcagaat attttggcag ggtcaagttt agttttctgg atgccgacga tcccaacact
     1921 tataatttcc agcgcgaact cgggttcgtg taccagcccg aattttattt actagatgcg
     1981 aatggaaatg tattgaagaa attggtcggt cccgtttctg aagaagaatt tagagatttg
     2041 ttcgatacga atttgcagta agcaggttta aaagagacag tcaactgact gtctcttttt
     2101 tgtttaactc caatataatt ccggctcatg gatacagcct ttcacatcat cgttttcttg
     2161 ctcggcgcag ggttggtcat tgctaccatt ttttctgcta tttcaacatt cgttttgcca
     2221 cgttcggcac gcagccaact taaccggctg gtgtttgggc taacccgtcg ctttattgaa
     2281 atcttcatgc attttgctcg aacctatgag caacgagatg ctatcatggc gtattatgcg
     2341 cctttggcac tgatgtcgct tgtgcctact tggtacatgt tgattgcgct tgggtattct
     2401 ttcatgtatt ggtctttacg ggctggggat tatttctttg atctgcgctt gagcggctct
     2461 tctttattta cattgggatt taccgcctca gagggatatt tcatcaccat cctcgccttc
     2521 tccgaagcca tgctaggact catcatggtg ggcttgctga tcgcttattt gccaacgatg
     2581 tattctgctt tttcacgccg tgagcaggcg gtgaatatgt tggaggtccg cgcgggcaac
     2641 ccgccgaatc cttacgatat gctctcccgc tttcatcgca ttcacggttt ggataaactc
     2701 gcagaactat ggaagaactg ggaaatctgg tttgccgacg tggaagagag tcataccact
     2761 ctgcccgctt tggtcttttt tcgctcgcct cgttccgata attcttgggt cacttctgct
     2821 ggagccgttt tagattcggc tgccattgcc ctttcatcaa ttgatattcc ttccgaaatg
     2881 tctggtgctt tgtgtattcg ggctggtttt atttgttttc atcgcatcac agattatttt
     2941 gatattccac acccgcacga ccccaaatac ccagagacgc ccatcagtgt cactcgcgcc
     3001 gaatatgacg agttgattca taaactggaa gttgccggtg tgcccatcaa gccagaccgc
     3061 gaacaagctt ggaaagattt cgccggatgg cgagtcaact atgaccgtac gctgttgatt
     3121 atgtgcgcat tggtgatgcc accctccaac gtcccatggt ccagcgaccg ggctccgaaa
     3181 ttcaaaatcc ctccattgtt tataaagaaa acaaagcatc aacattaaat aaaaagagtg
     3241 ccgaga
//
LOCUS       DAOREF010000284         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19275_length_3241_cov_3.915882, whole genome shotgun sequence.
ACCESSION   DAOREF010000284 DAOREF010000000
VERSION     DAOREF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19275_length_3241_cov_3.915882"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1110)
                     /locus_tag="PKI33_07065"
     CDS             complement(10..1110)
                     /locus_tag="PKI33_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM36802.1"
                     /translation="MTETPSANLLDSLDLNKRFKTSAYFTICFNSLPSSLHLVLSSDK
                     KQDAVDAILAEMELEKFNFTTVFKNWSTEDFQNSQPSDDYVFEYVFKSEAGRMLIHFT
                     ARDLSYITVEFLYDHDQKGSEEWVLAMNHKLRSKFGSPKKPQFKVLMHQDGMFGMEEV
                     STNDFKSIDIAELYNDDFVEVDKAISTFMGKQESGIILLHGEPGTGKTTYIKHLICKF
                     IDREFIFIQNDFVSDLLKPNFIAFLLQNKNAVLIIEDAEKVVVPREHSSEASVVSTIL
                     QLTDGLFSDFLNIKIICTFNTDIERIDKALLRKGRMIAKYNFSALSPAKTAALAQKLG
                     HEHVEGSLTLADIFGYDKLDFKEAAKRKIGFV"
     gene            1224..2585
                     /locus_tag="PKI33_07070"
     CDS             1224..2585
                     /locus_tag="PKI33_07070"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNM36803.1"
                     /translation="MNTTLDRSKLEKLLKQEEELFHKTHPKSYELYQRARKSLHGGVP
                     MLWMIRWAGSFPVFVKEAKGAHFTDVDGNNYIDFCLGDTGAMTGHAPEATVKAITEQI
                     QKGITLMLPYEDVIWVGEELQRRFKLPYWQFALTATDANRFALRMARLITGRPKVLVF
                     NYCYHGSVDETFITLDEEGTPISRPNNMGPQIDPRETSKVIEFNDIAALETALSARDV
                     AVVLAEPAMTNIGIIHPDPGYHEALRALTRKYGTYLIIDETHTICAGPGGYTASYGLE
                     PDFITLGKPLAGGVPAAIYGFTEEVSKAFSAKLAMDDADVGGIGGTLAGNALSIAAMK
                     ATLQNVLTDEFYAKATTLQEQFTAGVEGVIEEFRLPWIVKRLGNRSEYWFRPTPPKNG
                     GEAHAAVDPELDRYMHLYALNRGILMTPFHNMALISPETTQDDVDHHTKVFREAVKSL
                     FGE"
     gene            2588..>3241
                     /locus_tag="PKI33_07075"
     CDS             2588..>3241
                     /locus_tag="PKI33_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNM36804.1"
                     /translation="MTDYKSQIWRINTRTQELKREPVPETWQRLGGRGLIARIMVDEV
                     DATCDPLGPGNKLIFTPGLLVGHILSSTDRISVGGKSPLTGGIKEANAGGRTGYHMAF
                     MGIHALILEDMPEKDGYWVLHLSLNDGAKWERADELVGLGVYETAPKLIEKYGDKVAI
                     SLIGPGGEMRMKSAGIQNLDKDKIPSRIAARGGLGAVMGSKGLKAIVFDHIGGQKPPI
                     "
BASE COUNT          820 a          729 c          827 g          865 t
ORIGIN      
        1 aggctctgtt tagacgaatc cgatcttgcg tttggcggct tctttgaaat cgagtttgtc
       61 atagccgaag atgtctgcaa gggtgaggct gccttccaca tgttcgtgac ctagtttttg
      121 ggcgagggca gccgtctttg caggggagag cgcagagaag ttgtacttgg caatcatacg
      181 ccctttgcgt aagagagctt tgtcaatgcg ctcgatatcg gtgttgaagg tgcagatgat
      241 cttgatgttg agaaagtcgc tgaagagacc gtcggtcaat tgcaaaatgg ttgagacgac
      301 ggatgcttca gaggagtgtt ctcgcggcac gacaactttc tctgcatctt caatgatgag
      361 aactgcattc ttgttttgca gtagaaaagc tatgaaattt ggtttcaaca agtcgctgac
      421 gaaatcattt tgaataaaga tgaattcgcg gtcgataaac ttgcagatca ggtgtttgat
      481 gtaagtggtc ttgccggttc cgggctcacc atgcaaaagg atgatgccag actcttgctt
      541 gcccatgaat gtggaaatgg ctttatccac ttcgacaaag tcatcattgt agagttcggc
      601 tatgtcaata gatttgaaat catttgtgct cacttcttcc atgccaaaca ttccgtcctg
      661 atgcataagg actttgaatt gtggcttttt gggtgatcca aactttgagc gtaatttgtg
      721 gttcatcgcc agtacccatt cttcactgcc tttttgatca tggtcgtaaa gaaattctac
      781 tgtgatgtac gacaggtcgc gagccgtaaa gtgaatgagc atgcgcccag cttcactttt
      841 gaaaacatac tcgaacacat agtcgtcgct gggttgtgaa ttctgaaaat cttcagtaga
      901 ccaattcttg aacacggtgg tgaagttgaa tttttccaac tccatttcgg caaggatggc
      961 atctacggca tcttgtttct tgtcactgga taacacaagg tgcaagctcg aaggcaatga
     1021 gttaaaacag atggtgaaat aggcagaagt cttgaaacgt ttgttcaagt ctaatgagtc
     1081 gagcaggttg gcagaaggcg tttcagtcat atatagtatt ttctccaaat agaggcggcg
     1141 cacgggttga tagtccgccc cctcgcttcg gtaatataca gtcgatttga gattttgaca
     1201 atcctgaaaa aatagagaga accatgaaca ctacccttga tcgttccaaa ctcgaaaaac
     1261 ttctcaaaca agaagaagaa ctctttcata agacccaccc caaatcgtat gagctgtatc
     1321 aacgggcgcg caaatctctg catggtggtg tgcccatgtt gtggatgatt cgctgggcgg
     1381 ggtcttttcc ggtgtttgtc aaagaagcca aaggggcaca ttttacagac gtggatggca
     1441 acaactatat cgacttttgt ctcggcgata cgggcgccat gacaggtcac gctcccgaag
     1501 cgacggtcaa agctattact gagcaaattc aaaagggcat taccctaatg ttgccctatg
     1561 aagatgtcat ttgggtaggt gaggaacttc aacgccgctt caagttgccg tattggcaat
     1621 ttgctcttac tgctacagat gcgaatcgct ttgcccttcg tatggcacgc cttatcacag
     1681 ggcgccccaa ggttttagtt ttcaactatt gctatcacgg ttctgtcgat gaaaccttca
     1741 tcacgcttga tgaagaagga acgcccatct ctcgccccaa caatatgggt ccgcagatcg
     1801 acccgcgcga aaccagcaag gtcattgaat tcaatgatat tgccgccctc gaaacggcgc
     1861 tctccgctcg cgatgtggca gttgtgcttg ccgaacctgc catgacgaat attggcatca
     1921 tccaccctga ccccggctat catgaagctt tgcgtgcatt gacgcgcaaa tacgggacct
     1981 atctcatcat tgacgagacg cacaccattt gtgctggccc cggcggatac accgcttctt
     2041 acggtcttga gcctgatttc atcaccctcg gcaaacctct cgctggaggc gttcccgccg
     2101 cgatctatgg ttttacagag gaagtctcga aagcattttc tgctaaactt gctatggacg
     2161 atgccgatgt cggtggtatt ggtggcacat tggcaggtaa tgctctgtcc attgcggcga
     2221 tgaaggcaac acttcagaat gtgctcactg atgagttcta tgccaaagcg acgacgttac
     2281 aggaacagtt tacagcggga gtcgaaggcg tgattgaaga attcaggctt ccgtggattg
     2341 tgaagcgttt aggaaatcgc tctgaatact ggttccgtcc cacacctcca aagaacggcg
     2401 gcgaagctca tgctgccgta gatccagaat tggatcgtta catgcacttg tatgcgctaa
     2461 atcgcggtat attgatgacg ccctttcata acatggcgct catctcacct gaaaccactc
     2521 aagatgatgt ggatcatcat acaaaagttt ttagagaagc tgtgaagagt ttgttcggag
     2581 agtgataatg actgactata aatctcaaat ctggcgtatt aatactcgta ctcaagaatt
     2641 gaagcgtgaa cctgtccctg aaacctggca acgcctcggc gggcgcggac tcattgcacg
     2701 catcatggtg gatgaagtgg atgcaacgtg tgacccactt ggacccggca ataagttgat
     2761 ttttacaccc ggcttgttag tagggcatat cctttcctcc acagaccgta tttcggtagg
     2821 cggtaagtct ccgctgacgg gcggaatcaa ggaagccaat gcgggcgggc gcacgggcta
     2881 tcacatggcg tttatgggta tccatgcact cattcttgaa gatatgccgg aaaaagatgg
     2941 ctactgggtt ttgcacttgt ctttgaacga tggcgcaaaa tgggagcgag ccgatgagct
     3001 tgttggcttg ggtgtgtatg aaactgctcc gaaactgatc gaaaaatatg gcgataaagt
     3061 tgccatttca ctcatcggtc ctggtggcga gatgcgtatg aagtcagcag ggattcagaa
     3121 tctagacaaa gataaaatcc cttcgcgtat tgctgcgcgt ggcgggttag gtgcggtgat
     3181 gggctcgaag ggtttgaaag ctattgtgtt tgaccacata ggcggacaaa aacctccaat
     3241 t
//
LOCUS       DAOREF010000285         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19302_length_3238_cov_3.560478, whole genome shotgun sequence.
ACCESSION   DAOREF010000285 DAOREF010000000
VERSION     DAOREF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19302_length_3238_cov_3.560478"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKI33_07080"
     CDS             <1..279
                     /locus_tag="PKI33_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36805.1"
                     /translation="LLNGLVTLPAIYFAEINPDDVDVMSLPQGGWTNNENMTRLVEKI
                     RASSCTKKAMVEAEQHVDRALVCLESFEPCAERDALENLAKYIVDRKV"
     gene            346..855
                     /locus_tag="PKI33_07085"
     CDS             346..855
                     /locus_tag="PKI33_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNM36806.1"
                     /translation="MTNPRKPFRFNVGFIIHEEVGYNHDFPFEFEKVTLNGDLELRNL
                     EGNVNVGKTPQGLIVQGKFTAETTVTCSRCLTDVVQDLEWEFTEMYAFDQRSITESGL
                     LLPEDAQLDVESLLREYALLEVPINPICKPDCLGLCIECGQNLNEKDCGHRPEEPDSP
                     FAKLKDLLK"
     gene            863..2101
                     /locus_tag="PKI33_07090"
     CDS             863..2101
                     /locus_tag="PKI33_07090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNM36807.1"
                     /translation="MPPAYAGGFHFSMKLSIPKPIRILITILVLVVLIFIRVRINLGF
                     DVPNSNFSFFWLAGRMILDGQNPYDETQYLAGHDANSMDWRPNNIFPYPLPLALFCIP
                     LGFFSMQTAYIIWQIVTLLLIALTIYLLLQHWQEESQQNLLLPIFFFLLFLAPVYLST
                     HAGTISILPVMVLVGAILLVEKEQSLWAGVVLALTMLKPPQGLTILILAGVWFLARRD
                     WKAIIGVALGGLAILVVGLIQDPQWIQKFLGAGDAVMARTLGVHSNVWAFAYLACKGA
                     EPCSNLLGGALSLSLLGGASLLLWKNQAKWSAWEAMNLIIPIGFVSTIYLWAYDQLPY
                     IIPIVWIVGTLAQKQRSILLAFFFLILLDLASIFALVQQAFTEKDLWSLSTTLIVLLF
                     MFVAWRMKPKPAIDKAPASA"
     gene            2258..>3238
                     /locus_tag="PKI33_07095"
     CDS             2258..>3238
                     /locus_tag="PKI33_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNM36808.1"
                     /translation="MFEKNRYSKVELRISKPEYRIIEERSSTMTETILVAVAWPYANA
                     EIHVGNITGSPLPGDIFARYNRLKGNKVLMITGTDSHGTPVTMSADKLGKPVEEVYKS
                     FHEGFIELFQQIGITYDLYTTTHSDNHFKVSQAMFLALQKNGYLFKEFSKQWYSPSAG
                     KFLPDRYVEGTCYICGFEGARSDQCDNCGNVLEPEKLKNPRAKTGDGALELRDTEHYY
                     LDLSKLEPEVKKFLQARSDHMRDTVIGESLRKIESEGLKARSITRDIDWGIPVPVNEP
                     EWETKKLYVWFEAVIGYLSAPIEWAQLAGNKEAWRDWWINPAAKQFHFIGK"
BASE COUNT          777 a          835 c          785 g          841 t
ORIGIN      
        1 ctactgaatg gcttggtcac cctccctgcc atttacttcg ctgagatcaa tcccgatgac
       61 gtggatgtga tgtctctacc ccaaggcggt tggaccaata acgaaaacat gacccgtctc
      121 gtggaaaaaa tccgcgccag tagctgcacc aagaaagcca tggtcgaagc cgaacaacat
      181 gtagaccggg cactcgtctg ccttgaaagt tttgaaccgt gtgccgaacg cgatgccttg
      241 gaaaacttgg caaagtatat tgttgataga aaagtctaag aatcaggaag cctgatttaa
      301 cagaccctga tttactagtc tcttattcct aatcccttat ttcccatgac caaccctcgc
      361 aaaccctttc gttttaacgt cggcttcatt attcatgaag aagtcggcta taaccacgac
      421 tttccgttcg agttcgagaa ggtcactctc aacggtgact tggaactccg caacctggaa
      481 ggcaatgtca acgttggcaa aacccctcag ggattgatcg ttcaaggcaa attcaccgct
      541 gaaaccaccg tcacttgttc acgttgtctc accgacgttg tacaagacct tgaatgggaa
      601 ttcaccgaaa tgtacgcctt cgaccaacgg tctattacag agtctggttt gctgttgccc
      661 gaagatgctc aactagatgt agagagcctt ctgcgcgagt atgcgctttt agaagtgccc
      721 atcaacccga tttgcaaacc cgattgtctc ggtctgtgca ttgaatgcgg tcaaaacctc
      781 aacgagaaag attgcggtca ccgccccgaa gaacccgatt cgccttttgc aaaattgaag
      841 gacttgctta agtaaaacct gcatgccacc tgcttacgcg ggtggttttc atttttctat
      901 gaaactttcc attcctaagc ctattcgcat cctcatcaca atcctcgttc tggtagtttt
      961 gatcttcatc cgcgttcgga ttaacctcgg gttcgatgtc cctaactcca atttttcttt
     1021 cttctggctg gcagggcgga tgattttgga tggacaaaac ccatacgacg agactcaata
     1081 tctcgccggg catgatgcta acagcatgga ttggcgccca aataatattt tcccttatcc
     1141 actacccttg gctttattct gtattccgct cggcttcttt tccatgcaga cggcgtacat
     1201 catttggcag attgttacgc ttttgctgat tgccctgacc atttaccttc tgttgcaaca
     1261 ctggcaagag gaaagtcagc agaatttatt gctgcctata tttttcttct tgctcttttt
     1321 agctccggtc tacctgagca ctcatgcagg gacgatcagc atcctgcctg tgatggtgtt
     1381 ggtaggtgca attctcttag tggaaaaaga acaatcttta tgggcgggtg tcgtccttgc
     1441 gttgacaatg ctaaaaccgc ctcaagggct taccattttg atccttgccg gggtatggtt
     1501 ccttgcacgg cgtgactgga aagcaatcat cggcgtggcg cttggcgggc ttgcgattct
     1561 cgtggtgggt ttgatccaag acccgcagtg gattcaaaaa ttcctcggcg caggcgatgc
     1621 cgttatggcg cggactctcg gagtgcactc gaacgtttgg gcgtttgcct atctcgcctg
     1681 caagggagcg gagccatgct caaatctatt gggcggggca ttgagtttga gtctgcttgg
     1741 aggggcgagt ttgctccttt ggaagaatca agccaaatgg tcggcatggg aggcgatgaa
     1801 cttaatcatc ccgattgggt ttgtgagcac aatttatctc tgggcgtatg accagcttcc
     1861 atacatcatc ccaattgttt ggatcgtggg tacgttggcg caaaagcaac gcagtatttt
     1921 gctggcgttc ttctttttga tcttgctgga tctggcttcg atctttgcgc tcgttcagca
     1981 agcctttact gaaaaagact tgtggagcct cagcacgacg ttgatcgttt tgctctttat
     2041 gttcgtggcg tggcgcatga aaccgaaacc tgccattgac aaagcgcccg cctctgccta
     2101 aaataacgga catgaaattc acttccgctt tcccaatggt gccaatgacc atggccatgc
     2161 gcagcatgat gcgaatggaa atgtcccctt gcgccaagtg gaaagcatag tttttcatac
     2221 ctgaaaaaac gggctgtcca tttcgcggac agcctttttg tttgaaaaga accgatattc
     2281 gaaagtcgaa cttcgaatat cgaaacccga atatcgaatt atcgaggaac ggagttccac
     2341 catgaccgaa accattttag tagccgtcgc ctggccctat gccaatgccg aaattcacgt
     2401 gggcaatatc acgggttcac ccctgcccgg tgatattttc gcccgttaca accgcctcaa
     2461 gggcaacaaa gtgttgatga ttaccggcac cgactcacat ggtacgcccg tcaccatgag
     2521 cgcggacaag ctcggcaagc cggtcgagga agtgtacaag tcattccatg agggcttcat
     2581 cgagttgttc cagcagatcg gcatcaccta cgacctgtac accaccaccc acagcgacaa
     2641 tcacttcaaa gtttcgcagg caatgttcct tgccttgcag aagaatggat acctcttcaa
     2701 ggaattcagt aagcagtggt actcccccag cgcgggcaag ttcctgccgg accgttacgt
     2761 cgaagggact tgttatatct gcggcttcga aggtgcacgt tctgatcaat gcgataactg
     2821 cggcaatgtg ctcgaacctg agaaattgaa aaatccgcgc gcgaagaccg gagatggtgc
     2881 gctcgaacta cgcgacacgg aacattatta tctcgacctc tccaagcttg agcctgaggt
     2941 taaaaaattc ttgcaggctc gttccgacca catgagagat actgtgatcg gcgagtcact
     3001 gcgcaagatc gaaagcgaag ggctcaaagc ccgctccatc acccgtgata ttgattgggg
     3061 cattccggtt cccgtcaatg aaccggaatg ggagacgaag aaactctatg tgtggttcga
     3121 agctgtcatc ggctatctct ccgctccgat cgagtgggcg cagctcgcag ggaataaaga
     3181 ggcgtggcgc gattggtgga tcaaccccgc cgcgaagcaa ttccacttca ttggcaaa
//
LOCUS       DAOREF010000286         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19389_length_3229_cov_4.152804, whole genome shotgun sequence.
ACCESSION   DAOREF010000286 DAOREF010000000
VERSION     DAOREF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19389_length_3229_cov_4.152804"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..422)
                     /locus_tag="PKI33_07100"
     CDS             complement(39..422)
                     /locus_tag="PKI33_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36809.1"
                     /translation="MNSFPSAIVVEDDPYLLDIYADTLRGMNIQVETYQDGMSALKGL
                     KNATPNLIILDLNLPKLSGIEIFHALRNQPQTAETWVLIITANPAQAAELSKNEIESQ
                     NLLILTKPISVDQLDQLAQRIIFHS"
     gene            complement(415..2211)
                     /locus_tag="PKI33_07105"
     CDS             complement(415..2211)
                     /locus_tag="PKI33_07105"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNM36810.1"
                     /translation="MQPTPLLYPWLLSVTLTFLTGLYAFRYRRHVAAIPFILMCFFAS
                     FWALCYIFELGATSLELKTWGVKLGYIGILGLPLSWTAFALAYSGHTSWITKRNIMLA
                     LIFPIITLIVIFTNQYHHWFFTDFGLQTDSASGLILIYNPLGWWFWLHAVYTYGILIF
                     GSYFLVREYWEKREVYRSQIIINMTAILLPWISNGIVLAGLLPVRIDITSVMFSISIL
                     IFGWGFLRYQLLDILPVAHRAVFESISDSVIVLDPELRIIELNPAAVEMFKLNIGEVL
                     GKSFQSVFRPWVQLNDKALQTHGYHREIVLENDGEPVRWLHLYVSALRNGASTTEGHI
                     ITLRDVTSIKENESALAIARDEAMQANSFKSQLLANVSHELRTPLGIILGYTDLIVRR
                     SYGDLTEKQVNILGRIKDSTQYLDGLVSELLDQAQLDSGKLHLSTRPFEPREVLGSVC
                     NQLSILAEAKNLEFHYSISENMPISIVGDSQRLKQILVNLISNAIKFTEVGRITVNIA
                     VLPSNTEWIMQVIDTGPGIPEESLGTVFEPFKQLANAHKSLRKGYGLGLSIARQLIRL
                     MGGNISLESALGKGTTFTITLPLVVETEDNDE"
     gene            2384..>3229
                     /locus_tag="PKI33_07110"
     CDS             2384..>3229
                     /locus_tag="PKI33_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36811.1"
                     /translation="MSTTVMQPGLPGKSRSKVVTKPATDFYGGIGWVIGLLLTLFVSA
                     PVIWFLTQNASRADRADILAYGLAAAWWLNPLSLGGLFYVFSNIRMWRFWTLPALFVW
                     LLINAMVSASIASDVAREWVLNALFIGGLVILGLLFLYIQLRVFVWAASGNASRFFFG
                     IIGWALLNGLVVWALVNPDVLNIVLVALGFIFRVVFAIGFAIIQFVAIFWFMSQTRVE
                     IIRPGDPKQITFDDYKGQPNLLKMVRQWIYLLSDREQFQKMGGQFITGLLLYGEPGTG
                     KTLLAK"
BASE COUNT          836 a          690 c          802 g          901 t
ORIGIN      
        1 tttaaggtaa agagtcattt aagagcagaa caatgagttt agctgtgaaa aataatgcgc
       61 tgagcaagtt ggtcgagctg gtctacgctg atcggctttg tgaggatgag cagattttgg
      121 ctttcaattt cgttcttaga aagttcggcg gcttgagcgg ggttggcggt gatgatcaag
      181 acccatgttt cagcggtttg gggttggttg cgaagcgcat gaaagatttc gatgccagaa
      241 agtttcggaa gattgagatc gagaatgatg aggttgggcg tagcattttt gagtcctttc
      301 aaggcgctca tgccatcttg ataggtctca acctgaatgt tcatgccgcg taaagtatcg
      361 gcatagatat ccaagaggta tgggtcgtct tctacgacga ttgcactggg aaaactattc
      421 atcgttatct tctgtttcaa ctacaagagg gagggtgatg gtgaaggtag tgcctttgcc
      481 aagggcgctt tcaagcgaga tattcccgcc catgagtcgg ataagttgcc tggctattga
      541 aagccccaag ccataaccct tgcgcaggga tttatgggca tttgctaatt gcttgaatgg
      601 ttcgaaaaca gtgccaagcg actcttccgg gatgcctgga cctgtgtcta tgacttgcat
      661 gatccattct gtgttggagg gcaatactgc gatgttgacc gtaatcctgc caacttctgt
      721 gaacttgatt gcattgctga tgagattgac aaggatttgc tttagccgtt gactatctcc
      781 gacaatggaa atgggcatat tctctgaaat ggaataatga aactcaaggt tttttgcctc
      841 agccagaatg cttagttgat tacaaaccga cccaagcact tcacgcggct caaacggacg
      901 tgtggagaga tgtaattttc cgctgtcaag ttgggcttgg tcgagaagtt cagaaaccag
      961 tccatctaaa tattgggtgc tgtctttaat gcgccccaga atgttgactt gtttttcggt
     1021 caaatcgccg taggatctac gtacgatcag gtctgtgtac ccgaggatga taccgagcgg
     1081 agtccgcagt tcgtggctta cgttggcaag taattgagat ttgaagctat ttgcctgcat
     1141 ggcttcatca cgggcaatgg ctaatgcaga ttcattttct ttgatactgg tgacatcgcg
     1201 taaggtgatg atatgccctt cagtggtcga tgcaccattg cgcaaagcgc ttacataaag
     1261 gtgtaaccaa cgtacaggct cgccatcatt ttccaatacg atctcgcgat gatacccatg
     1321 ggtttgcaag gctttatcat ttaactgcac ccaaggtctg aagacagatt gaaaagattt
     1381 cccaagcacc tcgccaatgt ttaatttgaa catctccacg gcggcgggat taagctcaat
     1441 aatgcgtaat tctgggtcga ggacaatgac cgagtcggag atactttcaa agacggcgcg
     1501 gtgggcaaca ggcagaatat ccagcagttg atagcgcaga aagccccacc cgaagatcaa
     1561 gattgagatg gagaacatga ccgaggtaat gtcgatgcga actggaagta gtccagcaag
     1621 cacaatacca tttgatatcc acggcagaag aattgcagtc atgttgataa tgatctgaga
     1681 gcgatacact tcgcgttttt cccaatattc gcgaacaagg aaataagagc cgaagatcaa
     1741 aatgccatag gtataaacgg catgcaacca aaaccaccaa cctaaaggat tatagatcag
     1801 aattaatcca gaagcgctat cggtttgtaa gccgaaatct gtgaagaacc agtgatggta
     1861 ttgattggta aagatcacaa tgagcgtgat aatgggaaaa atcaacgcca gcatgatgtt
     1921 acgtttagtg atccagcttg tgtgaccaga atacgccagt gcaaaagccg tccatgaaag
     1981 tggaagacca agaatgccga tataccccaa tttcacaccc catgttttta attccaagct
     2041 tgtagcgcct aattcaaaga tataacaaag cgcccaaaat gatgcgaaga agcacatcaa
     2101 aatgaacgga atagcagcta catgcctgcg gtaacgaaaa gcatatagtc ctgttaggaa
     2161 agtaagcgtt acagacagta gccaaggata aagtagaggt gtgggctgca tttgaacaat
     2221 tatacaactt gataccgggg taaaagaatt gcgggaacat aattaggcga aagcgtaaga
     2281 atcacccttt ttgtgcatcc aagaactaat agccagctct tctcatttct ttatcacgtt
     2341 ttcaagataa acttgcccca atcttctttc gctcagaggt ctcatgtcca caaccgttat
     2401 gcaaccgggc ttacccggta agtcacgttc caaagtggta accaagcctg ccactgattt
     2461 ttatggggga attggatggg tcattgggct tttgctcact cttttcgttt cagctccagt
     2521 catttggttt ttgacgcaga acgcctcgcg cgccgaccgg gcagatatcc tcgcctatgg
     2581 cttggcagcc gcctggtggc ttaatccgct atcgctcggc gggttgttct atgtttttag
     2641 caacattcgt atgtggcgct tttggacgct ccccgctctg ttcgtttggc tattgatcaa
     2701 tgccatggtg tctgcgtcca ttgcttctga tgtggctcgt gaatgggtct tgaacgccct
     2761 tttcattggc ggtttagtca ttctaggttt gctgttttta tacattcaat tacgcgtatt
     2821 tgtttgggcg gcatctggca atgccagccg ctttttcttc ggcattattg gttgggcctt
     2881 acttaatggt ttggttgttt gggcattggt taaccctgat gtgctgaata ttgttcttgt
     2941 tgccttgggc tttattttcc gcgttgtatt tgccattggc ttcgccatca tccaatttgt
     3001 ggcgatcttc tggttcatgt ctcaaacacg tgtggagatc attcgccccg gagacccgaa
     3061 gcagattacc tttgacgatt acaaaggtca gccgaacctg cttaagatgg tccgccagtg
     3121 gatttatcta ttgtctgacc gtgaacaatt ccaaaaaatg ggcggacaat tcatcaccgg
     3181 cttgttgctc tatggtgagc ctggtacggg taaaaccttg ctagccaaa
//
LOCUS       DAOREF010000287         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19414_length_3227_cov_4.790984, whole genome shotgun sequence.
ACCESSION   DAOREF010000287 DAOREF010000000
VERSION     DAOREF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19414_length_3227_cov_4.790984"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1504
                     /locus_tag="PKI33_07115"
     CDS             <1..>1504
                     /locus_tag="PKI33_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNM36812.1"
                     /translation="ANVIRRLKRVAKFYGANPQFILASATIGNPKELAETLIEEPVEL
                     VDNDGSSRGERHFIIYNPPVLNPALGLRKSSILESVRLANDLLKHDVQSVVFARTRRS
                     VEILLTHLQTQIVNLKSKIENPIRGYRSGYLPTQRREIEQGLRDGSVKTVVATNALEL
                     GIDIGGMGAALMVGYPGTIASARQQAGRAGRGDLPAVSILVASPNPLDQFLAHHPEYF
                     FDRSPEMALVNPNHLLILLEHLRCAMFELPFQRGEGFGSISDELVDEYLEFLVSNQEA
                     HFSNEKYFWMQDAYPAANISLRSASPQSVVLHTTADDGRPTIVGTVDGESANWMVHPG
                     AIYMHEAQQYFVQELDLESHIAHLVPVGLDYYTEAQQQSEIQITALNEQLATRGCEKG
                     YGEIQVTTQVVGFRKIRWFTNENLGQEPLDLPPSELQTTGYWIVLSEAALKVLRDAGL
                     WTNDPNDYGPDWQKIRLAVRKRDQFKCQVCGAAETNREHDVHHKTPFRAF"
     assembly_gap    1505..1604
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1605..1855)
                     /locus_tag="PKI33_07120"
     CDS             complement(<1605..1855)
                     /locus_tag="PKI33_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNM36813.1"
                     /translation="MTNDEWLSLAGAADLLGVHPSTVRLWSDKGLLPVHKTQGGHRRY
                     KRGEILLWAESNEKSKEEALEPEGMMQEVIKNVRMQISEG"
     gene            complement(1883..3187)
                     /locus_tag="PKI33_07125"
     CDS             complement(1883..3187)
                     /locus_tag="PKI33_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HNM36814.1"
                     /translation="MFIKRLQLALIHTAVAMTLVPINSTLNRVMIFDLGVSKTLFTLL
                     AIFPYLLSPIQVAIGSFSDRHPILGYRRAPYIFAGLLLCVIGVAVSPQIAILISNNFT
                     VGVAAGILAFGLWGMGYNLSAVSYLSLASELSGEKERGKTVATMFTLMVIGLIATGIS
                     LSQMVPTFEPATLQRAFLIIAASALTMGLIGLIKLEPRFDHSAQAPKADTYTVKQMMA
                     AITENPVAKIFFVYLLLLLAAILSQDVLLEPFGAQAFGMTLEQTSRIVSITNSFTLIA
                     FIIAGFLDGRVKKKYVAQTGNLAALFGFIVIVISGLIANKNAFYVGITLLGFGTGIST
                     IANLSLMFDLTVPEKVGLYIGAWGFSNGLSRLVGLLMAGVIADVATKITGDALHGYLV
                     VFGLEALMLFIAAIMLYRIDVGVFQKKAHEPGFAEKIALASE"
BASE COUNT          805 a          775 c          830 g          717 t
ORIGIN      
        1 tcgccaacgt catccgcagg ctcaaacggg tggcaaaatt ctatggcgcg aatccacaat
       61 tcatccttgc ttcggcaacc attggcaacc caaaagaatt agccgagacg cttattgaag
      121 aacccgttga attggtggat aacgacggtt cctctcgcgg cgagcgtcat ttcattatct
      181 ataacccgcc tgtgttaaat cctgcattgg gcttgcgcaa gagcagcatc cttgaatcgg
      241 tacgcttggc gaatgatttg ctgaaacatg atgtgcagtc ggtcgtcttt gcgcggacac
      301 gccgtagtgt ggaaatcctt ctaacccatc tacaaacaca aatcgtaaat ctgaaatcga
      361 aaatcgaaaa cccgattcgc ggctatcgct ctggctacct cccaacccaa cgccgcgaga
      421 tcgagcaggg acttcgtgat ggctcggtca agaccgttgt ggcaaccaat gcgctcgaac
      481 tcggcattga cattggcggc atgggggctg ccctcatggt tggctacccc ggcaccattg
      541 caagtgcgcg ccagcaggca gggcgagcgg gacgtggcga cctgcctgcg gtttcgatcc
      601 ttgttgcttc accgaatccg ttggatcaat tcctcgcgca tcatcccgaa tacttttttg
      661 atcgttcgcc agagatggca ttggtcaacc cgaatcattt gttgatttta ctggagcatt
      721 tacgttgcgc catgttcgaa ctgccatttc agcgtggcga aggcttcggc tccatttctg
      781 atgaattagt ggatgagtat cttgagtttt tggtctccaa ccaagaagcg catttctcga
      841 acgaaaaata tttttggatg caagatgcct atcccgccgc gaatatttct ttgcgttctg
      901 cctccccgca aagtgtggtg ctgcacacca ctgcagatga tggtcgcccc accattgtag
      961 gaactgtaga tggcgagtct gccaattgga tggtacaccc cggcgcgatc tatatgcacg
     1021 aagcccaaca atactttgtg caggaacttg accttgagag ccatatcgca caccttgtgc
     1081 cggttggctt ggattactat accgaagccc aacagcaatc tgaaattcag atcacagcat
     1141 tgaatgagca acttgccacc cgcggctgtg aaaagggata tggcgagatt caagtcacga
     1201 cacaagtcgt aggctttcgc aagatccgct ggttcacgaa tgagaatctt gggcaggaac
     1261 cgcttgacct tccgccttct gaattacaaa cgaccggcta ttggatcgtc ctctcagaag
     1321 cagcgcttaa agtgttgcgt gatgctggtc tatggaccaa tgacccgaac gactacggac
     1381 ccgattggca aaagataaga ctcgccgtca gaaaacggga ccagttcaaa tgtcaggtgt
     1441 gcggcgcggc ggagacgaat cgcgagcatg atgtgcatca caaaactccc ttccgtgcct
     1501 tcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccttca gagatttgca
     1621 tgcggacgtt cttgatgact tcttgcatca tgccttcggg ttcaagcgcc tcttctttgg
     1681 acttttcatt tgattctgcc caaaggagaa tttctccacg cttatagcgg cgatgccccc
     1741 cctgagtctt atgaacgggg agtaagcctt tatcagacca taatcgcacc gtgctcgggt
     1801 gaacccccag cagatcggcg gcaccagcga gagaaagcca ttcatcgttt gtcatgttca
     1861 gccgatatcc tgctttttca aactattctg aggcaagcgc gatcttttcg gcaaacccag
     1921 gttcatgcgc cttcttttgg aaaacgccca catcaatgcg gtacaacatg atcgcagcga
     1981 tgaacaacat caatgcctct aacccaaaga ccaccagata gccatgaagg gcatcaccag
     2041 tgattttggt tgctacgtct gcgatgacac ctgccatgag tagaccaact aaacgcgaga
     2101 gtccgttcga gaagccccac gcgccaatat acaaaccaac cttttcgggg acggtcaggt
     2161 caaacatgag ggagaggttg gcgatggtgg aaattccggt gccaaacccg agcagggtaa
     2221 tgccaacata aaaggcattt ttgttggcga tcaacccgct aatgacgata acaatgaagc
     2281 cgaacaatgc ggcgaggttg cctgtttgtg caacgtactt cttttttaca cgtccatcga
     2341 gaaaacctgc aatgatgaag gcgatcaatg tgaacgaatt agtaatggag acgatgcgcg
     2401 aggtctgttc gagagtcatg ccgaaggctt gagcaccaaa gggttcgagc agaacatcct
     2461 gactcaagat ggcggcgaga agcaagagta ggtacacgaa aaatattttc gctacggggt
     2521 tttcagtaat ggcagccatc atttgcttga cggtgtacgt atccgctttt ggggcttggg
     2581 cagagtggtc gaaacgtggt tccagtttga tgagaccgat caatcccatg gtcagggctg
     2641 aggcggcgat gatcaggaag gcgcgctgaa gggtggcagg ttcaaaggtg ggcaccatct
     2701 gactcaagct gatgcccgtc gcgatcaacc cgatgaccat gagtgtgaac atggtggcta
     2761 cggtcttacc gcgttctttt tcgccggaaa gttcagatgc cagcgaaaga tatgaaacag
     2821 cagagaggtt gtagcccatg ccccataaac caaaggcgag aatgccagca gccaccccaa
     2881 cggtaaagtt gttgctaatg aggatggcga tctgcggtga gacggcaacc ccgatcacac
     2941 acaaaagcag accagcgaaa atgtatggcg cgcggcgata ccctagaatg gggtgacggt
     3001 ctgagaagga gccaatggca acttgaatgg gggaaagcaa atagggaaag atggcaagca
     3061 gtgtaaaaag ggtttttgag acgcctagat cgaagatcat gacccggttg agggtgctgt
     3121 tgatgggcac aagcgtcatg gcaacagcgg tatggatgag ggcaagttga agtcttttga
     3181 tgaacatagc atttcctgag gttgaggatt aaacaaaagg aagggcg
//
LOCUS       DAOREF010000288         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19416_length_3227_cov_2.940416, whole genome shotgun sequence.
ACCESSION   DAOREF010000288 DAOREF010000000
VERSION     DAOREF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19416_length_3227_cov_2.940416"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1102)
                     /locus_tag="PKI33_07130"
     CDS             complement(65..1102)
                     /locus_tag="PKI33_07130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNM36815.1"
                     /translation="MTLFSEINEQPERIKSLLSSQRKHVEKIAAEIQKRGVDYVFLAA
                     RGTSDNAGRYANYLLGAVNGLPLALATPSLFTYYKKPPMLKNALVIGVSQSGKSPDIV
                     SVLEEGRRQGCMTLSITNEPNSPLAQKSDFVLDLQAGVEKAVAATKTYTTELMSIAML
                     SAALSGNKKAWDDLGNVSKWMRETLKQSDFIAQAVQRYRYIDQTVVLGRGFNYATAFE
                     WALKLKELTYIIAEPYSSADFAHGPIAMVESGYPVLAVAARGKVFDSMLEMLKRLRSD
                     IAAELVVISNDRKALSLAQVPLQIPVDTPEWLSPLVSILLAQLFAYHLTLAKGYNTEQ
                     PRSIRKVTETK"
     gene            complement(1122..2699)
                     /locus_tag="PKI33_07135"
     CDS             complement(1122..2699)
                     /locus_tag="PKI33_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNM36816.1"
                     /translation="MDLEHQIGQKLLLAFRGKESLSPEIIQAFKNYHPSGVTLFRPFN
                     IESPAQIKKLNQSLQDLARELGLPLLLIATDQEGGQLMAFGDGTPLPGNMALGATRSP
                     ELARKAGEVLGRELAAIGVNVNYAPSADVNINPNNPVVGVRSFGEDPQLVGELAAAMI
                     EGIQSQGVAATVKHFPGHGDTASDSHHGLGTVPHSRERLHAVELTPFRSALQADAKLV
                     MTAHLGILSIDGKDAPPATLSPNILTGLLRDELDFKGVIITDAMDMHAIRQGELLRED
                     SLRAAQAGADILLMTTDPQDQARAFEALLQGAKSGELSQEKLQASLGRISELKKWLME
                     NATSPELGVIQCAEHMLVANEIAEKSITLVRDEKNYLPLKLEAGKRIAVIIPVPQDLT
                     PADTSSYIQPMLAESLRKYHSLTDEFKIPYAPSADEATKVLEQVTEYDVIVMGTINAF
                     AEEKQAEFVLQLLKLDKPVINIALRLPYDLAAFPQASTFVCTYSILEPSMRAVAKAVF
                     GHSEMTGRLPVSIPGLE"
     gene            complement(2701..>3227)
                     /locus_tag="PKI33_07140"
     CDS             complement(2701..>3227)
                     /locus_tag="PKI33_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNM36817.1"
                     /translation="VVFFLMLSTMMIPPQALIVPQYVFFNKLDWIGTFNPLIIPGYFA
                     GGAAMIFLLRQSMSQIPKELDESAFVDGANHIQIWWEIILPLVKAPMATVATFLFVGL
                     WNSLLTPLMYLQTVDLYTLPVYVSTLYNINQTVQPWPTIMTAAVLTTAPLIFVFFFAQ
                     RFILESVVVSGLKG"
BASE COUNT          863 a          835 c          763 g          766 t
ORIGIN      
        1 attatccacc catttccgat tcagcaattg attttttaag aaaaatagag cagattcctc
       61 tgctctattt ggtttccgtc accttgcgga tgctgcgtgg ctgctctgtg ttgtacccct
      121 tggcaagtgt caaatgatag gcaaacaatt gagcaagcaa aatgctcacc aatggagaaa
      181 gccattctgg cgtatcaacc ggaatctgca gtggaacttg cgccaacgac agagccttcc
      241 ggtcgttcga gatcaccacc aactcggcag caatatcaga acgcaggcgt ttgagcatct
      301 caagcatcga atcaaagacc ttacctctgg ctgccactgc caaaacaggg tagccgctct
      361 ccaccatggc aatcggacca tgcgcaaaat cggcagaaga atatggctcg gcaataatat
      421 aggtcagctc ttttagtttc aatgcccatt caaatgcggt cgcataatta aacccgcgcc
      481 ccaagacaac cgtttgatca atataacgat agcgctgcac agcctgggca ataaaatcgc
      541 tttgcttgag cgtttcacgc atccactttg aaacgttacc gagatcatcc cacgcctttt
      601 tatttccgct caatgccgcc gaaagcatcg cgatcgacat caactcagtc gtataggtct
      661 ttgtagctgc aactgctttc tctaccccgg cttgaagatc caacacaaaa tctgatttct
      721 gcgccaacgg cgaattcggc tcgttcgtga tcgaaagcgt catgcagcct tgccgccgcc
      781 cttcctccag cacactcaca atatccggcg acttgcccga ttgcgaaaca ccaatcacca
      841 atgcattctt caacattggc ggttttttgt aataggtgaa caaagaaggt gtcgccaacg
      901 caagcggaag tccattcacg gcaccaagca aataattggc ataacgcccg gcattatccg
      961 atgtcccgcg agcggcgaga aagacataat ccacgcctct cttttggatt tccgccgcaa
     1021 ttttttctac atgcttgcgc tgcgaagaca gcaggctttt gatacgctcc ggctgttcat
     1081 tgatttcaga aaataaagtc atgatgaaaa tactccacgc ctcattccaa gccgggaatg
     1141 gataccggca aacgccccgt catctcgcta tgaccgaata cggctttagc caccgcccgc
     1201 atcgacggtt ccaaaatgga ataggtacac acgaaggtcg aagcctgcgg aaatgcagca
     1261 aggtcatacg gcaggcgcaa ggcaatattg ataacgggtt tatctaactt aagcaactgg
     1321 agcacgaact cagcttgctt ttcttcggca aaggcattga tcgtacccat cacaatcaca
     1381 tcatactccg tcacctgctc caatacctta gtcgcctcat ctgcacttgg tgcatacggg
     1441 attttgaatt catcggtcag agagtgatat ttacgaagcg actcagcaag catgggttga
     1501 atgtaagaag aagtatctgc cggggtcaag tcctgtggaa cagggatgat gacggcaatc
     1561 ctttttccag cttcgagttt gagaggcaga tagttcttct cgtcacggac cagggtaatg
     1621 gacttctcag caatctcatt cgccactagc atatgttcag cacattgaat aactcccaat
     1681 tcaggggaag tggcattctc catcagccat tttttcaact ctgaaattcg tcctaacgaa
     1741 gcttgtaatt tttcttgcga gagctctcca cttttcgcac cttgcaataa ggcttcaaat
     1801 gctcgcgctt ggtcttgcgg gtctgtggtc atgagcaaaa tatccgcacc agcttgggca
     1861 gcacgcaagg agtcttcgcg cagtaactct ccctgacgga tagcgtgcat gtccatcgca
     1921 tccgtgatga tgacaccttt gaaatcgagt tcatcgcgca ataagcctgt gagtatattg
     1981 ggagaaaggg tggcgggagg cgcatcttta ccatcgatgg agagaatgcc gaggtgagcg
     2041 gtcatcacca gtttagcatc cgcttgcagc gcggagcgga acggcgtcaa ctctacagcg
     2101 tggagtcgct cacgcgagtg cggcacagtt ccaagtccat gatgtgaatc gctggcggta
     2161 tctccgtgac caggaaaatg cttgactgtt gccgccactc cttgagattg aatcccttcg
     2221 atcatggcag ccgccagttc ccctaccaac tgtggatctt cgccaaacga gcgcacaccc
     2281 acaacgggat tgttcgggtt gatgttaaca tcggcactag gtgcgtaatt aacattgaca
     2341 ccaatcgccg ctaactcacg cccaagcact tcgcctgctt tccttgcaag ttcgggtgag
     2401 cgtgttgcac caagagccat gttgccaggc aagggggtac catcgccgaa cgccatgagt
     2461 tgaccaccct cttggtcagt ggcgatcaac aaaagcggca gacccaactc acgcgcgagg
     2521 tcttgcagcg attggtttag ctttttgatc tgcgcgggcg actctatatt aaatggacga
     2581 aagagcgtca caccgctggg atgataattt ttaaatgcct gaataatctc aggcgaaaga
     2641 ctttccttgc cacgaaatgc caacaaaagt ttttgaccga tctgatgttc gagatccacg
     2701 ttaacctttc aagccagaaa cgacgacact ctcgagaatg aatcgttgag cgaaaaagaa
     2761 tacgaaaata agtggagccg tggtcagcac ggcggcggtc atgatggtgg gccaaggctg
     2821 aactgtttgg ttgatgttgt acagagtgga aacatagaca ggcaatgtgt aaagatcaac
     2881 agtctgcaag tacatgagtg gtgtaagcag gctattccac aaacctacaa agaggaaagt
     2941 ggcaacggtt gccatcggcg ccttaaccaa gggcagaatg atctcccacc aaatttgaat
     3001 atgatttgct ccatccacga atgcggattc atctaactct tttggaatct gtgacatgga
     3061 ctgccgcagt aaaaatatca tcgccgcgcc gcccgcaaaa taaccgggaa tgatgagcgg
     3121 attgaaagta ccaatccagt ctaacttatt gaagaaaaca tactgtggca caatcagagc
     3181 ctgtggcggg atcatcatag tggaaagcat caggaagaag actacgt
//
LOCUS       DAOREF010000289         3221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19469_length_3221_cov_4.941251, whole genome shotgun sequence.
ACCESSION   DAOREF010000289 DAOREF010000000
VERSION     DAOREF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19469_length_3221_cov_4.941251"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKI33_07145"
     CDS             <1..366
                     /locus_tag="PKI33_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36818.1"
                     /translation="SFLISSREPIAHEKASLTEVIQGNTKHGFFGFFMFGGILAFYVV
                     TEILVSSRLALYMRQYFNMDLKQSSLYVTYFFSFLLLGRLGFTFKKFAASLKMQMNIL
                     LGLTFFCVLLGLFAHPFFL"
     gene            complement(369..1238)
                     /locus_tag="PKI33_07150"
     CDS             complement(369..1238)
                     /locus_tag="PKI33_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNM36819.1"
                     /translation="MEYWPVVVIILQLIFLEGILSIDNAAVIGALVSPLPNDEEVPWP
                     GKLTWLGKALHPILGFQRMAALRVGLLGAYVGRGAMLFFTSFLIHNSWIKLVGAAYLI
                     HLAFDNLDDMAMGGDENGEVSPIEARSFWATVLTVEIMDLVFSIDNVVAAVSLSDKLW
                     VVMLGVGIGILTMRYAAGIFSYAVEKEPILKQAAYILVLNIGVELILSQVWKIEIPDL
                     LRFGISVATIVLTLAYAHIPFLQRFRFVLLWFAQGIGIINKLVDWLFMPIKGIFSLIA
                     GLFSRPSNSQPAE"
     gene            1360..1968
                     /locus_tag="PKI33_07155"
     CDS             1360..1968
                     /locus_tag="PKI33_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36820.1"
                     /translation="MKNFKSPLTYFFLTVGLIALLAFFGPEEKSLGSNVRLVYIHGAW
                     VLTAEIAFIFAALAGLLGLLLRRDLFHTWSAALGRTGIIFWVTYLPLSLLAMQTNWNG
                     LFLAEPRFRLAMIFAVTGVLLQLGLWMFSIPWLTSLANIFYIIVLRVIFAQAQNIMHP
                     PPSPIFNSGNYVIIGFFVSLNLLAWLAAYFLTRFLLNLKPQN"
     gene            1977..3125
                     /locus_tag="PKI33_07160"
     CDS             1977..3125
                     /locus_tag="PKI33_07160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36821.1"
                     /translation="MKLRFQLAIFLFVRTVLNSIHRMVYPFLPIFARGLGIDLSTMSL
                     LMTGRSLLGATSPFLGPISDWRGRKFGMLSGLGIFILGIGLVALFPTFWALVIALGLA
                     LIGKYIFDPAMQAYFGDRIPYHQRGTVLAVTEVSWSMAFIVGVPIAGFLIDRYGWAAP
                     FPLFSILGLIAFFILWRIIPNEDSHHEPVNNTREGYAAILKSMPALAGVSIALWASAA
                     NELVNVIFGVWLEDSFGLKIAALAGASAVIGISELSGEGLVALTTDKLGKPRALAIGL
                     VANSLAAGLLPFIGRTQVGALIGLFLFYITFEYVMVSHIPMVTELVPAARATLLSLNV
                     TGHALGRALGAFLAAIIYQQFGFIFVALSAVVFNIIGIFALRKMQQAE"
BASE COUNT          815 a          766 c          659 g          981 t
ORIGIN      
        1 tcttttttga ttagttcacg cgaacctatt gcgcacgaaa aagcgtcgct tacagaggtg
       61 attcaaggaa acaccaagca cggcttcttc ggctttttta tgtttggtgg aattttagct
      121 ttctatgtag taaccgaaat tttagtttcg tcgcggctag ccttatacat gcggcagtat
      181 tttaacatgg acttaaaaca gtcgagtttg tacgtgacgt atttctttag ttttttattg
      241 ttaggtcgat tgggttttac ttttaaaaag tttgcggcat ctttaaagat gcaaatgaat
      301 attttgctgg gccttacttt tttttgtgtt ctattggggc tttttgctca tccttttttt
      361 ctgtaaaatt attctgctgg ttgtgaattt gatggacgtg aaaaaagacc cgcgattaaa
      421 ctaaagatgc ctttgatcgg cataaagagc caatcaacca gtttgttgat aattccaatt
      481 ccctgagcga accacagcaa aacaaagcgg aagcgctgta agaaagggat atgtgcatac
      541 gccagtgtca atacaatggt cgcaactgaa atcccaaacc gcagcaaatc agggatttca
      601 attttccaca cctgcgaaag gatgagttca acaccaatat tcaaaacaag gatatacgca
      661 gcctgcttca gaatgggctc tttctctacg gcataactaa aaataccagc ggcataccgc
      721 atggtcaaaa tgccaatgcc tacacccaac attaccaccc ataatttatc agaaagcgaa
      781 accgccgcta caacattatc aatgctaaag accaagtcca ttatttcaac ggttaaaact
      841 gtagcccaga atgaacgcgc ttcaatagga gaaacctccc cattttcatc tccacccatt
      901 gccatgtcgt caagattatc aaatgccaaa tggatcaaat aagcagcgcc caccagctta
      961 atccacgaat tgtggatgag aaagctagtg aagaaaagca tagctccacg ccccacatag
     1021 gcacctaata agcccacacg caaggctgcc atacgctgaa aaccgaggat gggatgcaat
     1081 gccttcccaa gccatgtcaa tttaccgggc caaggaactt cttcatcatt cggtaacggc
     1141 gacaccaacg caccgatcac agccgcatta tcaattgaaa gaatcccctc taaaaaaatt
     1201 aactgcaaaa taattacaac aacgggccaa tattccatat cacaaggagc ctttcactcc
     1261 ataaactcgc aagtatggat tctatcccaa accgcaaaga ttttcctctt tcttttgaaa
     1321 atttttgtaa caaataaata gcaatgttaa aatccaacca tgaaaaactt caaatcccct
     1381 ctcacctatt tctttttaac cgtaggcttg atcgcccttc tagccttctt tggtcccgaa
     1441 gaaaaatcat taggatcaaa cgtccgcctc gtttacatac atggagcttg ggtactcacc
     1501 gctgaaatcg cctttatttt cgcggctctt gccggacttt taggcttgct cctgcgacgc
     1561 gaccttttcc acacctggag cgcagctctt ggtcgcacag gcattatttt ttgggtcaca
     1621 tatctgcctt tgtccttgct agccatgcag accaactgga acggtctctt tcttgctgag
     1681 ccgcggtttc gtcttgccat gatctttgct gttacaggtg tcctactcca attgggtctg
     1741 tggatgttca gtattccgtg gctcacatcg ctggcgaata ttttttatat tattgttctg
     1801 cgggttatct ttgcgcaagc ccaaaacatc atgcacccgc ctccatcccc aatctttaac
     1861 tctggaaatt acgtcatcat cggctttttc gtatcgttga atctgcttgc ttggcttgca
     1921 gcttatttcc tcacccgctt cttgcttaac ctcaaaccgc agaactgagc attcttgtga
     1981 agctacgctt tcaacttgcc atctttttat tcgtccgcac tgtgttaaat tcaattcaca
     2041 gaatggtcta tccgttcttg cctatctttg ctcgtgggct tggcatagac ctctctacca
     2101 tgtctctgct tatgactggg cgttcccttc ttggcgctac cagcccattt ctaggtccca
     2161 tttccgattg gcgtggacga aaatttggca tgttgtctgg cttgggaatt tttattctag
     2221 gcattggtct tgtggcgctc ttccctacct tttgggcatt ggtcatcgca ttagggctgg
     2281 cactcatcgg caaatatatt tttgaccctg ccatgcaagc ctattttggc gatcgcattc
     2341 cttatcacca acgcggaacc gtccttgcag ttacagaagt ttcctggtct atggcgttca
     2401 ttgtaggtgt gcccattgca gggtttctaa ttgaccgtta tggatgggcc gcgccattcc
     2461 ccttattcag cattttaggg ttgatcgcct tttttattct ctggcgtatc atccccaatg
     2521 aagattcgca tcatgagcca gtcaataata cccgtgaagg ttatgcagct attcttaagt
     2581 caatgcctgc actagcgggt gtttctattg cgttatgggc tagtgcagca aatgaattgg
     2641 tcaatgtgat ttttggtgtc tggttggaag attcatttgg gttgaaaatc gcggctttgg
     2701 ctggtgcttc ggcagttatt ggcatttcag aattgagcgg tgagggtctt gtggctctta
     2761 ctaccgataa gctaggaaaa ccgcgtgctc ttgctatcgg cttggttgct aattcattag
     2821 cagctggctt actcccgttt attggacgta ctcaagttgg agcactcatt ggtctgtttc
     2881 tcttctacat tacgttcgag tatgtcatgg ttagccatat ccccatggtg acagaacttg
     2941 tccctgctgc acgtgcaact ctgctatcgc tcaatgtaac tggtcatgcc ttaggacgcg
     3001 cactcggcgc atttttagcc gcaataatct accaacagtt tggattcata tttgttgcat
     3061 tgagtgctgt tgtgtttaac attataggaa tctttgcact gagaaaaatg caacaagcag
     3121 agtaaatgcc caatggggag atacaatttg tactaagaat gaagcgaaac aaaaagtccc
     3181 atcagatgat gggactttta taatgcaagt ctagagcgcg t
//
LOCUS       DAOREF010000290         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19503_length_3218_cov_3.093582, whole genome shotgun sequence.
ACCESSION   DAOREF010000290 DAOREF010000000
VERSION     DAOREF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19503_length_3218_cov_3.093582"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKI33_07165"
     CDS             complement(<1..858)
                     /locus_tag="PKI33_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNM36822.1"
                     /translation="MPNYLISASSPYLLQHANNPVDWYPWGEEALNKAKTENKPIFLS
                     IGYAACHWCHVMAHESFEDSETAAFMNEHFINIKVDREERPDLDNIYMQATIAMTGSG
                     GWPMSVFLTPELKPFYAGTYFPPVRRYNMPSFREVLEGLSNAWVNDREEIEKTSNKVF
                     EHLDTKLKSNNEENLTTDHLEAIAKAMQGAYYWGTGGWGDAPKFPQPMALEFLLHHAV
                     TTKQNEYIKLIEHCLKVMTRGGMYDVVGGGFSRYSTDDHWRVPHFEKMLYDNAQLVRV
                     YLHAWQVTHD"
     gene            978..>3218
                     /locus_tag="PKI33_07170"
     CDS             978..>3218
                     /locus_tag="PKI33_07170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNM36823.1"
                     /translation="MDTNPNASLEADHTHSISVRSLVEFVMQSGDLTAGGFQRRDRAQ
                     LGTLGHKQVQKSRPEGYDTEVEIICRIENTNPPLEIRGRIDGLYAGKEPVIIEEIKTT
                     TLSLDLIHDDHNPLHWGQAQCYAYMYALQQNLSEINVHLTYYQLDSHEEKTFERHFTW
                     AELESFFSNLITQYLDWFRKVQAWQARRDRSIEQLEFPYDEYRPGQRDLAVTVYKTIR
                     ANDRAYIQSPTGVGKTIATLFPSVKALGQGLATKIFYLTAKTSGRLVAEKALEDMYRS
                     NLYLRSVTLTAKEKICFCPPVNCDPEVCIFARGYFDKVKLALQELDQHQTFTRPVIEE
                     LARKYEICPFEFSLDVALWVDCIICDYNYVFDPRVYLHRFFDFTTEPYIFLVDEAHNL
                     PDRARAMYSAELDKETVLSLQRTLKPHVPALAKKLGEVNKILLEKRKACEAEDKDALI
                     EHDLPETLLKTLREFSHKAEDWLVMNQVAEFRQELLEFYFLCSNFLRTAEYFDTYYVS
                     YFERRGQSDLRAKLFCLDPAPLLAFPLERSCATIFFSATLLPMNYFKKLLTGATDQIS
                     RTFESPFPQENACLLIHNKISTKYTQRADSYDDIAELIETIIKTHTGNYLVYFPSYVY
                     LNEVVERLKQRLPDRQLLIQERGMDENARDSFLAQFSTTNEETLVGFAVMGGVFGEGI
                     DLVGDRLIGVIVVGVGVPQLGLERDLIKEYFDRDMGRGYAYAYQYPGFNRVLQATGRV
                     IRTDTDR"
BASE COUNT          874 a          651 c          763 g          930 t
ORIGIN      
        1 gtcgtgtgtc acttgccagg catgcaaata cacgcggacg agttgagcgt tgtcgtaaag
       61 cattttctca aaatggggca cgcgccaatg gtcatctgtg ctatagcgcg agaagccacc
      121 gccaacaaca tcatacatcc cgccgcgtgt cattactttg aggcaatgtt cgataagttt
      181 tatgtattca ttttgctttg ttgtgacggc gtgatggagc aagaattcaa gagccatcgg
      241 ctgtggaaat tttggcgcat caccccaacc gccagttccc cagtaataag caccttgcat
      301 tgctttggca attgcttcga gatgatctgt cgtaagattt tcttcgttgt tggattttag
      361 ttttgtatct aaatgttcaa agactttgtt gctagttttc tcaatttctt cgcggtcatt
      421 tacccaagca ttggagagac cttctaacac ttccctgaaa gagggcatgt tataacgtcg
      481 cacgggcggg aagtaggtgc cagcataaaa tggtttgagt tcgggagtaa ggaagacaga
      541 catgggccag ccgccagagc cggtcatggc gatggttgcc tgcatgtaga tattatcgag
      601 gtcgggtcgt tcttcgcggt ctactttgat attaatgaaa tgttcgttca tgaaagcggc
      661 ggtctcagaa tcttcgaaag attcatgagc cattacatga caccaatgac atgcggcgta
      721 ccctatgctt agaaaaatcg gtttattttc ggttttggct ttattgagag cttcttctcc
      781 ccatgggtac caatctacag gattgtttgc gtgttgaagt aaatatggag aactcgctga
      841 aataagatag ttaggcatgt gtaacctcta tagtaaatgt atacgttaac ttgtatggga
      901 ttatagctta tggtctagag caacgcttta taaatattaa aaagatattg tttatcgtgc
      961 tagaatccca tttgaaaatg gataccaacc ctaacgcttc attagaagca gaccatactc
     1021 atagcatttc tgttcgcagc ctagtggaat tcgtgatgca atctggcgac ctgacagcag
     1081 gcgggtttca aagacgcgat cgcgcccagc taggaactct gggacataaa caagtacaaa
     1141 aatcccgccc ggagggatat gacaccgaag tagaaatcat ttgtcgtatc gaaaatacca
     1201 atccgccatt ggaaatacga gggcggattg atggtttata tgctggcaaa gaaccggtca
     1261 tcattgaaga aatcaaaaca acaacactga gcttggatct gatacacgat gatcacaacc
     1321 cattgcattg gggacaagca caatgctatg catatatgta tgcccttcaa cagaacttaa
     1381 gtgaaatcaa tgtccatcta acttattacc aattggatag ccatgaagaa aaaacatttg
     1441 aacgccattt tacatgggca gaactggaaa gctttttctc gaacctaatt acccagtact
     1501 tggactggtt caggaaggtc caagcctggc aagctcggcg tgatcgttct attgagcagc
     1561 tcgaatttcc ctatgatgaa tatcgccctg gtcagcgaga tttggcagtc actgtctata
     1621 aaaccattcg tgcgaatgat cgtgcttata tccaatcgcc aacgggcgtg ggaaagacca
     1681 ttgcgaccct tttcccatcg gtcaaagcct tggggcaagg attggcaacc aagatttttt
     1741 atttaacagc caagacatca gggcgattgg ttgcggaaaa agctttagag gatatgtacc
     1801 gttcgaattt gtatctacga agcgtgacat tgactgccaa ggaaaaaatc tgtttttgcc
     1861 cgccagttaa ctgcgacccg gaagtatgta tatttgctcg tggctatttt gacaaagtga
     1921 aacttgcgct tcaagagctg gatcagcacc aaacttttac gcgcccggtt attgaagaac
     1981 tcgcccgaaa gtatgagatt tgtcccttcg agttttcgtt ggatgtagcg ttatgggtgg
     2041 attgcatcat ttgtgattac aactatgttt ttgatccgcg tgtctatctt catcgctttt
     2101 ttgacttcac gacagagcct tatatctttc ttgttgacga agctcataac ctaccagatc
     2161 gtgccagagc catgtactcg gctgagttgg ataaagagac tgtgctaagc cttcaacgta
     2221 ccttgaagcc acatgtgcct gctctggcga aaaagctggg agaggtcaac aaaatattat
     2281 tagagaaacg taaagcttgt gaggcagaag ataaagatgc cttgattgaa catgatttgc
     2341 ctgaaacctt gctcaagaca ctccgagaat ttagtcataa ggctgaagat tggcttgtta
     2401 tgaatcaggt ggctgaattt aggcaggagt tgttggagtt ttacttcctg tgcagtaatt
     2461 ttcttcgtac tgctgaatat tttgacacgt attatgtctc gtactttgaa cggcggggac
     2521 aatcggattt gcgtgccaaa ctattctgtc tggaccctgc tccactgtta gcgtttcctt
     2581 tggaacgtag ttgtgcgaca atctttttct ctgcgactct gttacccatg aattatttca
     2641 agaaactgtt gacaggtgca acagatcaaa tcagccgtac ttttgaatca ccttttccgc
     2701 aggaaaatgc ctgtttgtta attcataaca agatatccac taaatataca caacgtgcgg
     2761 attcttatga cgatattgcc gagttgattg aaaccatcat taaaacccat acgggtaatt
     2821 atttggttta tttcccatct tatgtgtact taaatgaggt cgtagaacgg ctcaagcaga
     2881 gattgccaga tagacagctt ctaattcagg aacggggaat ggatgaaaat gcccgcgatt
     2941 cttttctagc gcagttttcc acgaccaatg aagaaacgct ggtcgggttt gctgtgatgg
     3001 gaggtgtttt tggtgagggc attgatctgg ttggagatcg attgatcggg gttatcgttg
     3061 ttggagtggg tgtaccacaa ctgggattgg aaagagattt aataaaagaa tattttgacc
     3121 gtgatatggg tagggggtat gcgtatgctt atcaatatcc aggttttaat cgggtattac
     3181 aggcaaccgg gcgggtcatt cgcacggata cagatcga
//
LOCUS       DAOREF010000291         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19522_length_3216_cov_4.566593, whole genome shotgun sequence.
ACCESSION   DAOREF010000291 DAOREF010000000
VERSION     DAOREF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19522_length_3216_cov_4.566593"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1165)
                     /locus_tag="PKI33_07175"
     CDS             complement(95..1165)
                     /locus_tag="PKI33_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36824.1"
                     /translation="MNSIILIVDDNEASRETLESILASQNYDLHMAADGLQALEKANA
                     LFPDLILLDVMMPGMDGFEVCRRLRSTPALAEVPIILLTALDDTASRLEGIEAGADDF
                     ISKPFDRQELRARVRTITRLNRYHALLEQRENLREMAQRMVTGLEDERMRISRELHDE
                     LGQLLTAELMDIRDLQNDLTIPPVVLFERLEKIHAQAYEVTVKIRRLAHELRPPLLST
                     LGLKQAVETHCSKFSNHSHIPITVEVDDAVSALPDVYGIILYRVLQEALTNIAKHANA
                     DHAWVELSLEDDHINLIVQDNGRGLPTTETAASGIGVVGMKERAALVGGELSIRSGKN
                     GGTILSVSLPLKPGTTVKREGA"
     gene            complement(1162..>3216)
                     /locus_tag="PKI33_07180"
     CDS             complement(1162..>3216)
                     /locus_tag="PKI33_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM36825.1"
                     /translation="LYAHNHDHLRFTEALAFHVSAALSNAQLFSELEKRVRERTAEVQ
                     DLYDNAPAGYHSIDPSGRIILINQTELNWLGYSREEIVGHHISKVLTDAGLAKFQENF
                     PVFKYNGIIKDLELEMRRKDGSTLPALISATAIRDQEGNYLMSRSTVFDNTERKMADQ
                     ALREREEVYRALFENANDAIFMLKPDGTIIGANPHCMDILGIPYSELPGRNALDFIVN
                     TEFQAAEGQLRRLLAGERLPIYERTFKRKDGALIEAEINLSLISDEAGKPKFIQSVVR
                     DISTRKRAEETLHLANAEMQRALRLKDEFLANMSHELRTPLNAVLGITESLLEQISGP
                     LNEKQKKYLQTILESAQHLLELINDILDLAKVNAGRIELDINKIDIQTFAQSNLRMVR
                     ELAQKKNIKVNLEIAPTVKSAWADERRLKQMLVNLLSNAIKFTPSGGDIGLEINGDRT
                     EHTINFVVWDTGIGIKEDDLHLLFKPFVQLDAGLARGSQGTGLGLALVSQMARLHGGR
                     VLVESEPNKGSRFTITIPWVELSNTGPLTFPINSMPLPVEVQPEKNISQDYQILLVED
                     TESVTMLIGDYLKKHGYKIMTARDGFEGLARIREVKPDLILMDVMMPEMDGIETTRRI
                     REQLELKDIPIIALTALAMAGDRERCLEAGMNDYMSKPVKLKELLSIIEKYLKPGKEG
                     TQ"
BASE COUNT          861 a          764 c          642 g          949 t
ORIGIN      
        1 gcttcaccaa gaataaccat atcgccaact ttctctaaca gtgcacgaat ccccgcccgt
       61 accatcaaat gatcttccgc gatcaaaata tgaatcatgc tccctctctt tttactgtag
      121 ttccaggctt tagtggcaaa gatactgaaa gaatggtgcc accattcttt cctgaacgaa
      181 tacttaattc cccacccacc aatgcggctc gctctttcat acctacaaca ccaataccgc
      241 ttgcagcagt ctcagttgta ggtaagccgc gcccattgtc ttggacaatc aaattgatat
      301 ggtcatcttc cagacttaac tctacccagg catgatcggc attcgcatgc ttagcaatat
      361 tggtcagtgc ctcttgtaaa acccggtaca aaataattcc atatacatca gggagggctg
      421 agacagcatc atctacttca actgtaatgg gaatgtggct gtgatttgaa aatttagaac
      481 aatgggtctc tacagcttgt ttcaagccta atgtacttaa caaaggcgga cgtagttcat
      541 gcgcaagcct tcgaattttt acggtaacct catatgcctg tgcatggatt ttctctaatc
      601 gttcaaacaa aacaacaggc ggaatagtga ggtcgttttg caagtcgcgg atatccatca
      661 attcagccgt aagcagttga cctagttcgt catgaagctc acgtgaaatg cgcatacgtt
      721 catcttccaa ccctgtcacc atacgttgtg ccatctcgcg caggttttca cgttgttcta
      781 gcaaagcatg gtaacgatta aggcgagtga ttgtccgtac gcgcgcccgc aattcctgcc
      841 gatcaaatgg tttggaaata aagtcatcgg caccggcttc aattccttcc aaacgagagg
      901 cggtatcatc taaggcggtg agaaggatga tagggacctc cgcaagcgcc ggcgtagacc
      961 ggaggcgtcg acaaacctca aatccatcca tccctggcat cattacatcc agtaagatca
     1021 gatcggggaa taaggcgttc gctttttcca atgcttgcaa accatctgct gccatatgca
     1081 gatcataatt ttgcgaggca aggattgact ctagcgtctc tcggctggct tcattgtcat
     1141 ctacgatcaa aataatgctg ttcattgggt gccctcctta cctggtttta gatatttttc
     1201 aataatgctg aggagttctt ttaacttgac aggcttgctc atataatcat tcatgcccgc
     1261 ttctaggcaa cgttctcgat cgcctgccat agccagtgcg gtaagcgcaa taatagggat
     1321 atcttttagt tctaattgtt cgcgaatgcg acgggtagtt tcaataccat ccatctctgg
     1381 catcataaca tccattagta tcaggtctgg ttttacctcg cgaattcggg caagcccttc
     1441 aaaaccatcg cgggctgtca ttattttata gccatgtttc ttaagataat caccgatcaa
     1501 catcgtaaca gactctgtat cctccactaa aagaatttgg tagtcttgac tgatattctt
     1561 ttcaggttgc acttccactg gcaatggcat tgagtttata gggaatgtaa gtggtccagt
     1621 attactcaat tctacccatg gaatggtaat cgtaaaacgg cttcctttat tcggttcact
     1681 ctctactaat accctaccac catgcaagcg tgccatttgg ctgacgagtg caagccctaa
     1741 acctgttccc tgacttccac gtgccaatcc ggcatccaat tggacgaaag gtttgaaaag
     1801 caagtgaagg tcatcttctt ttataccaat gcccgtatcc catacaacaa agtttatggt
     1861 gtgttccgtt ctatcgccat tgatctcaag cccaatatct ccgcctgaag gagtaaattt
     1921 aatcgcatta ctcagcaggt ttactaacat ttgcttaaga cggcgttcat ctgcccaagc
     1981 agactttaca gtgggcgcaa tttccagatt tactttgata ttcttctttt gcgctaattc
     2041 acggaccata cgcaagtttg attgagcaaa ggtctgaata tcgattttat taatatccaa
     2101 ttcgatgcga cctgcgttga ctttggcaag gtccagaata tcgttgatca actccaatag
     2161 atgttgagcg ctttcaagaa tggtctggag gtatttcttt tgtttttcgt ttagaggacc
     2221 ggaaatttgt tcaagtaagc tttctgtaat ccctaagacc gcattaagcg gtgttcgcaa
     2281 ttcgtggctc atatttgcca aaaattcatc ttttaggcgt aaggcgcgtt gcatttcagc
     2341 attagcaaga tgcaatgttt cttcagctcg tttacgagtg gatatatcgc ggactacgct
     2401 ttgaataaat tttggttttc cagcttcatc agagatcaag gataggttaa tctctgcttc
     2461 aatcaaagca ccatctttcc gtttaaaagt ccgctcataa attggcaatc gttctccggc
     2521 taataaacgt cttaattgcc cctcagctgc ttgaaattca gtatttacga tgaaatcaag
     2581 agcatttctg cccggcaatt cagaatatgg aatccccaaa atatccatac agtgcgggtt
     2641 tgccccaata attgttccat ctggtttaag cataaagatg gcgtcattgg cattttcaaa
     2701 caaagcacga tagacctctt cacgctcacg aagtgcctga tctgccattt ttctttctgt
     2761 attatcaaac actgtagaac ggctcataag ataattacct tcttgatctc gaattgccgt
     2821 agcactgatc aacgctggca aggtagagcc atctttccga cgcatttcaa gctctaagtc
     2881 tttaattatg ccgttgtatt tgaataccgg gaaattctcc tgaaattttg ctaacccagc
     2941 atctgttaat acctttgaaa tatgatgccc cactatttct tcgcgggaat agccaagcca
     3001 attgagttca gtttggttaa taagaataat tcgcccgctg ggatcaatcg agtgataccc
     3061 agctggagcg ttatcataca gatcttgtac ctcggcggta cgctcgcgca cccgtttttc
     3121 aagttcagaa aacaattgcg cattcgataa tgcagcagaa acatgaaatg ccaaggcttc
     3181 tgtaaaacgc aaatggtcgt gattatgagc atataa
//
LOCUS       DAOREF010000292         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19527_length_3216_cov_3.516609, whole genome shotgun sequence.
ACCESSION   DAOREF010000292 DAOREF010000000
VERSION     DAOREF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19527_length_3216_cov_3.516609"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKI33_07185"
     CDS             <1..273
                     /locus_tag="PKI33_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36826.1"
                     /translation="IPGVIFGAATVVLLNIQVLQNFSLYLSGLRQSDAVIPIINFAWK
                     NLSNQLDPAKYQKLVFGLILILMMIFRPSGLIPAERRKRELGEEKE"
     gene            287..1075
                     /locus_tag="PKI33_07190"
     CDS             287..1075
                     /locus_tag="PKI33_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36827.1"
                     /translation="MALLQTTNVVKRFGGLTAVNKMTFSVDAGKIVSIIGPNGAGKTT
                     FFNSLTGIYKPEEGQILFKDNSLIGLRPDQVAATGIARTFQNIRLFGSMTVIENILVG
                     MHIQLKQGAVDTLFRSKKFNEEEKEAENRAKDLMAYVGLKNVGNELARNLPYGGQRRI
                     EIARALAANPTLLLLDEPTAGMNPNESEDAIKLFRRIRDEKGITILMIEHDMRVVMGI
                     SEHITVMDYGEKIAEGSPQDIRSNQRVIEAYLGRGAAVAHEQKV"
     gene            1086..>1650
                     /locus_tag="PKI33_07195"
     CDS             1086..>1650
                     /locus_tag="PKI33_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36828.1"
                     /translation="MGTLLELNNVHSYYGHIHALKGISLHVNEGEIVSLIGSNGAGKS
                     TSLRTISGIIHPREGKVELSGENISHMDAHRIVTKGVGHVPEGRGIFPKLTVQENLDM
                     GAYTVNDQKTIKERMELVYETFPRLKERIAQYGGTLSGGEQQMLAIGRGLMLNPRILM
                     LDEPSMGLAPVLVDLIFDTIKKLNEQGT"
     assembly_gap    1651..1750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1751..1957
                     /locus_tag="PKI33_07200"
     CDS             <1751..1957
                     /locus_tag="PKI33_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36829.1"
                     /translation="VLVDLIFDTIKKLNEQGTTILLVEQNALMALSIAHRGYVLQTGA
                     IALTDDAAKLKQNEMVQKTYLGIE"
     gene            2061..>3216
                     /locus_tag="PKI33_07205"
     CDS             2061..>3216
                     /locus_tag="PKI33_07205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM36830.1"
                     /translation="MTNLPKTADVIIIGGGVMGASAAYHLAKRGVKNILLLEKESHFG
                     TGATGRCAGGVRYQFSTEINVTLSKHSLPMIERFKEELGQDPGYHKYGYLLVATNEQI
                     VKEFEHNVAMQNRLGVQTELLSGDEVRRRLPLMRFDDALAGTFHHKDGTADPNSIVMG
                     YVTAAQKMGVTALTGVEVLGVNVNGGEIRSVKTNLGEVEARIVLNAAGPWSGLIGAMA
                     GVNIPITPLRRQMFTTNPLQEVPEDFPFVIDFAKSLYFHREGEGLLIGMSNQNEKPGF
                     DQNVDEDFELVNLEAAIERMPLIERASRASHWAGLYEVTPDAHPIYGATDVKGFLLCT
                     GFSGHGFMHGPISGKLMSEFIVDGKFSSVDVSMLDLKRFEEGRLINEYNVV"
BASE COUNT          827 a          783 c          793 g          713 t
ORIGIN      
        1 atccccggcg tgatcttcgg tgccgcaacc gtggtgctcc tcaacattca ggtgctccaa
       61 aacttctccc tctacctgag cggacttcgc caaagcgatg cagtcattcc aattatcaac
      121 ttcgcttgga agaacctctc caatcagttg gacccggcaa aatatcaaaa gctagtattt
      181 ggtttgatcc tcatcttaat gatgatcttt agaccctctg gcttgattcc agccgagcgc
      241 cgcaaacgcg aacttggcga agagaaagaa taggcggatt cctaccatgg ctctcttaca
      301 aaccaccaat gtagtaaaac gcttcggcgg tctgacagcc gtcaacaaaa tgaccttctc
      361 ggtggatgcc ggaaagatcg tcagcattat cggaccgaac ggcgccggaa agaccacctt
      421 cttcaacagc ctcaccggta tctacaaacc tgaagaaggg caaatccttt tcaaggacaa
      481 ttctttgatc ggcttgcgcc cagatcaggt tgcggctaca ggcattgccc gtaccttcca
      541 gaacattcgt ctgttcggta gcatgaccgt gattgagaat attctcgttg gtatgcacat
      601 ccagttgaaa caaggcgctg tagacactct cttccgctcc aagaaattta acgaggaaga
      661 aaaagaagct gaaaaccgcg ccaaagattt gatggcatat gtcggcttga agaatgttgg
      721 aaatgaactt gcgcgcaact tgccgtatgg tggtcagcgc cgcatcgaaa ttgctcgcgc
      781 tctcgctgcc aaccccaccc tgctcctgct cgatgaaccg accgccggta tgaacccgaa
      841 cgagtctgaa gatgcgatca agctcttccg ccgcatccgc gatgaaaagg gcatcaccat
      901 tctcatgatt gaacacgata tgcgcgtggt catgggtatt tctgaacaca tcaccgtgat
      961 ggactacggc gaaaagatcg cagaaggctc accacaggat attcgctcga accagcgcgt
     1021 gatcgaagcc tatctcggtc gcggtgctgc ggttgcacac gagcaaaagg tataggagat
     1081 tctgaatggg aacactactt gaactcaaca atgtccattc ctattacgga catatccatg
     1141 ccttgaaagg catttccctg catgtaaatg aaggcgaaat cgtctccttg attggttcca
     1201 atggcgcggg caagagtact tctctacgca ccatttctgg catcatccac ccgcgagaag
     1261 gcaaagtgga actttctggt gaaaacatat cgcatatgga cgcccatcgg atcgttacca
     1321 aaggcgtggg acatgtacca gaaggtcgcg gcatcttccc aaaacttact gtgcaggaaa
     1381 atctggacat gggtgcctat acggtgaacg accagaagac catcaaagaa cgcatggaac
     1441 tggtgtacga gaccttcccg cgcctcaagg aacgcatcgc tcagtatggc ggtacgcttt
     1501 ctggcggcga acagcaaatg cttgccatcg ggcgtggact tatgctcaac ccacgcattc
     1561 tcatgctcga tgaaccttcg atgggtctgg cccccgtgct cgtggacttg atcttcgaca
     1621 ccatcaagaa attgaatgag cagggcacca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn gtgctcgtgg acttgatctt cgacaccatc aagaaattga atgagcaggg
     1801 caccaccatt ctgctcgtcg aacagaacgc tttgatggcg ctctctatcg cccaccgcgg
     1861 atacgttctg caaaccggcg caatcgctct cacagacgat gcagccaagt tgaaacagaa
     1921 cgaaatggtc cagaagacct atctgggtat cgaatagaac gagacaagaa acaaacgaaa
     1981 aacccgtttt cacgcgaaag cgggtttttt tgtaaactgc gcacgcttac aaattgcgct
     2041 gggtataaaa taaaccaacc atgaccaatc ttcctaaaac agcagacgta atcattattg
     2101 gcggcggtgt aatgggtgcc agtgctgcct atcacctcgc aaaacgcggc gtgaagaaca
     2161 tcctccttct tgagaaagaa agccacttcg gcaccggtgc gacaggacgt tgcgcaggcg
     2221 gggtgcgtta ccagttctct acggaaatca atgtgacgtt gtcgaagcac agcttaccga
     2281 tgatagagcg cttcaaagag gaactggggc aagatcccgg ttatcacaag tatggctatt
     2341 tgttggtggc aaccaatgaa cagatcgtca aagaatttga acataatgtt gccatgcaaa
     2401 atagactggg tgtgcagacc gaacttttga gcggtgatga agtccgcagg cgtttgccgc
     2461 ttatgcgttt tgacgatgcg ttggcaggca ccttccatca caaagatggc acagcagacc
     2521 cgaacagcat cgtcatgggg tatgtcactg cggcacagaa gatgggtgtt actgcgctca
     2581 ccggcgtgga ggtacttgga gtcaacgtca acggcggaga gattcgcagt gtaaaaacaa
     2641 atttgggtga ggttgaggcg cgcatcgtcc ttaatgcagc gggaccgtgg tcgggtctta
     2701 tcggtgccat ggctggagtg aacatcccca tcactcccct acgccggcaa atgttcacaa
     2761 ccaatcccct gcaagaagta ccggaagatt ttccgttcgt gatcgatttt gcaaagtcgt
     2821 tatattttca tcgcgaaggc gaaggcttat tgatcggcat gagcaatcaa aatgaaaagc
     2881 cgggcttcga ccaaaatgtg gatgaagatt ttgagttggt gaatttagaa gcagcgattg
     2941 aacgaatgcc gttgattgaa cgtgccagcc gcgcctcgca ttgggcaggc ttatacgaag
     3001 tgacacccga tgcgcacccg atctatggcg cgacggacgt gaaaggcttt ttgctttgca
     3061 ccgggttttc gggacatggc tttatgcacg gaccgatctc tggcaaattg atgagtgagt
     3121 ttattgtgga tggaaaattc tcaagcgtgg atgtttcgat gcttgacctg aaacgcttcg
     3181 aagaaggtcg tttgataaac gagtacaatg tagttt
//
LOCUS       DAOREF010000293         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19616_length_3208_cov_3.357120, whole genome shotgun sequence.
ACCESSION   DAOREF010000293 DAOREF010000000
VERSION     DAOREF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19616_length_3208_cov_3.357120"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKI33_07210"
     CDS             <1..394
                     /locus_tag="PKI33_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM36831.1"
                     /translation="PNFIAFLLQNKNAVLIIEDAEKVVVPREHSSEASVVSTILQLTD
                     GLFSDFLNIKIICTFNTDIERIDKALLRKGRMVAKYNFSALSPTKTTALAKKLGHEHV
                     EGSLTLADIFGYDKLDFKETAKRKIGFV"
     gene            complement(391..1671)
                     /locus_tag="PKI33_07215"
     CDS             complement(391..1671)
                     /locus_tag="PKI33_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM36832.1"
                     /translation="MTNQLYQDNFSHVTPAWSRIFNFVADRAEGSYIYTDDGKKLLDF
                     TSGIGVTNTGHCHPKVVEAIREQAGLFLHAQANIVIHKPMLQLIEELRKVVPPSIDSF
                     YFANSGAEALENAVKIAKAVTGRQNIIVFNGSFHGRTHATMALTTSKTIYRTGFAPLP
                     AGIYVAPFPYAFNLKMTEEQASQYALEQLEYLLASQTAPKETAAILIESVLGEGGYVV
                     PPASFMKGLREICDKHGIMLIFDEVQSGFGRTGKWFALEHFGVVPDIITAAKGIASGM
                     PLSGVFSRTEIMKKVDTGSIGGTYGGNAVACAAGVATIRAMREEKMLENATERGIQLM
                     TGLHKLQEEYPQIGDVRGKGLMVGTEFIVDGSQAKAKPLVKEIIHKAEEKGLLLLSCG
                     TYDNILRWIPPLNVSSEQINAGLKIFSEAVKGSV"
     gene            complement(1759..>3208)
                     /locus_tag="PKI33_07220"
     CDS             complement(1759..>3208)
                     /locus_tag="PKI33_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNM36833.1"
                     /translation="VDPKAEILQHGIGAENGVLYSSLVSMSNRHNGRTGMGLVMASKN
                     LKAVVVRGTGKIKLANQKALTELNRIGPKAIPENGDMDGLAKFGTAVVVLFNNTIGTL
                     PTRNYNEGQFEGCEPISGEKMAETVLKERDTCYACVVKCKRVVEIKDGPYKVDPRYGG
                     PEYETLGTFGSYCGVDDLAAVSLANQICNEYAVDTIAAGATIAFAMECYEKGIITKEQ
                     TAGLDLKFGNAEAMIAALNMLVKGEGEFGKTLGLGSERAAAKWGNGADECLITVKGAE
                     APAHMPQAKRSLALIYAVNPFGADHQSSEHDPYYEEGVGDFNLDRLKQIGLGSPQPAY
                     SLTEEKVRFAYETEVFYSMLDSAELCQFVYGPTWTVYDGEQTAQMISSVTGWDITLNE
                     LMEVGRRRLDLFRVFNAREGLARNADKLPKKFFKELKGTGPTAGFALTHEEVESALDT
                     YYKMAGWTNNGIPTRETLKKHDVEWAAEYLPA"
BASE COUNT          825 a          863 c          711 g          809 t
ORIGIN      
        1 gccaaatttc atagccttcc tgctgcaaaa caagaatgcg gttctcatta ttgaagatgc
       61 agagaaagtc gtcgtaccac gagagcattc atccgaagca tcggtcgtct caaccatttt
      121 gcaactgacc gatggtctct tcagcgactt tctcaacatc aagattatct gtactttcaa
      181 cacagatatt gagcgcattg acaaagctct cttgcgcaaa gggcgcatgg ttgccaagta
      241 caacttttct gcgctctccc ctacaaagac aactgcactc gccaagaaac tggggcacga
      301 acatgtggaa ggcagcctca cccttgcaga catcttcggc tatgacaagc ttgatttcaa
      361 ggaaaccgcc aaacgcaaga tcgggttcgt ctaaacagag ccttttacag cctcgctaaa
      421 aatcttcaaa ccagcgttga tctgttcaga agagacattc aatggcggaa tccaacgcaa
      481 aatattatcg taggtaccac aggagagaag gagcaaaccc ttctcttctg ctttatggat
      541 gatctctttg accaacggct tggctttcgc ctgactgcca tccacgataa attcggtgcc
      601 aaccatcaag cctttaccgc gcacatcacc aatttgcgga tattcttcct gcaatttatg
      661 aagcccggtc atcaattgga ttccgcgttc cgtggcgttc tccaacatct tctcttcacg
      721 catcgcgcgg atggtagcaa cgccagccgc gcaggcaact gcgttcccac cataggtccc
      781 accaatggag ccagtatcca ctttcttcat gatctcggtg cgactaaaga cgcccgaaag
      841 cggcattcca gaggcaatcc cttttgcagc agtgataata tctggaacaa caccaaagtg
      901 ttccaacgca aaccacttgc ccgtgcgacc aaacccagat tgcacttcgt caaagatcag
      961 cataatgccg tgcttgtcgc agatctcgcg cagtcccttc atgaaggagg caggcggcac
     1021 cacatatcct ccctcaccca atacagactc gatcaagata gcggcggtct ctttaggagc
     1081 cgtttgagaa gctagcaaat attccaattg ctctaatgca tattgactgg cttgttcttc
     1141 ggtcatcttc aggttgaaag catatgggaa cggcgctacg taaatgccag caggcaaagg
     1201 cgcaaagccg gtgcgataga ttgtcttaga ggtggtcaat gccatagtgg catgcgtgcg
     1261 cccatgaaag gagccgttga agacaataat gttttgccta cccgtgacag ccttggcaat
     1321 cttcacggca ttttccaacg cctctgcgcc agagttggca aagtagaaac tatcaatcga
     1381 aggcggaact actttacgca gttcttcaat cagttgcaac atcggtttgt ggatgacgat
     1441 gttcgcctga gcatgcaaga acaagccagc ctgctcacga atcgcctcca ccactttggg
     1501 gtgacaatgc cccgtgtttg tgacgccaat tccactggtg aaatccagaa gtttcttgcc
     1561 gtcatcggta taaatatatg aaccctcagc acggtccgct acgaaattga aaatgcggct
     1621 ccaggcaggg gttacatgag aaaaattatc ttgatagagt tgattggtca tgatttcctt
     1681 gttgaccaga aagagcacag agttttccct gaatttcttc ttggactctg tgctctcagt
     1741 gatcagattg tatttacatt atgccggaag gtattccgct gcccactcta catcatgctt
     1801 cttcaaagtt tcgcgggtag gaatgccgtt attcgtccaa cctgccatct tgtaataggt
     1861 atcgagagca gattcaactt cttcatgagt cagagcgaag cccgctgtgg gacctgtccc
     1921 cttcaattcc ttgaagaatt tcttaggcaa tttatccgcg ttgcgagcca aaccttcacg
     1981 agcattgaag acacggaaga gatccaaacg tcggcgcccg acttccatca attcgtttag
     2041 ggtaatatcc caacccgtta ccgagctgat catctgtgca gtttgttcgc catcatagac
     2101 cgtccatgtc ggaccgtaca caaactggca gagttctgct gagtcgagca tcgagtaaaa
     2161 gacttcagtc tcgtatgcga agcggacttt ttcctctgta agtgagtatg caggttgggg
     2221 cgaacccaaa ccaatctgtt tgaggcggtc gaggttgaaa tcacctacac cttcttcata
     2281 gtacgggtcg tgttcagaag attggtgatc tgcgccgaag ggattgaccg cataaatgag
     2341 cgccaacgag cgtttggctt gcggcatgtg tgcaggagct tcagcgcctt tgaccgtgat
     2401 gaggcattca tctgcaccat ttccccactt ggcggcagca cgttctgagc ccaaaccaag
     2461 ggttttgccg aattcaccct cacccttcac cagcatattc aaagcagcga tcattgcttc
     2521 ggcattacca aatttcaagt caagaccagc ggtctgttct ttggtgataa tgcctttttc
     2581 atagcactcc atcgcaaaag cgatggttgc accagccgca atcgtatcta cagcatattc
     2641 gttacaaatc tggttggcaa gagataccgc cgcaagatca tccacaccgc agtatgaacc
     2701 aaacgtgcca agagtttcgt actcaggacc accatagcgc ggatcaacct tataaggacc
     2761 gtctttgatc tctaccacgc gtttgcattt gaccacgcat gcatagcaag tatcacgttc
     2821 cttcaagacc gtctcagcca ttttttcgcc gctgatcggt tcacaacctt cgaactgacc
     2881 ttcgttatag ttacgggttg gcaatgtacc aatggtgtta ttgaacaata ccaccactgc
     2941 cgtaccgaat ttcgcaagcc cgtccatatc accattttca ggaatggctt tcggaccgat
     3001 acgatttaac tcagtaagag ccttttgatt ggcaagttta atcttgccgg tacctcgcac
     3061 caccacagcc ttgagattct tagatgccat caccaagccc atgcccgtac gcccattatg
     3121 gcggttagac atagaaacga gggatgaata cagcacccca ttttcagcgc caatcccatg
     3181 ctgaaggatc tcagccttcg ggtccact
//
LOCUS       DAOREF010000294         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19695_length_3200_cov_4.248967, whole genome shotgun sequence.
ACCESSION   DAOREF010000294 DAOREF010000000
VERSION     DAOREF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19695_length_3200_cov_4.248967"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /gene="pstC"
                     /locus_tag="PKI33_07225"
     CDS             <1..784
                     /gene="pstC"
                     /locus_tag="PKI33_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNM36834.1"
                     /translation="LPALSEVEPSALFNIRWYPIESYFGILPLITGSLIVTLGAALIA
                     VPFGIGTAIYISEIAPRWMREILKPLVELLGGLPSVVLGFLGILVVSPNLRILLDLPT
                     GLTAFTGSLLLGGIAVPTVVSVAEDALDAVPRSYREGAWALGATPWQTIWRVTLPAAK
                     SGVLTAIMLGVGRAIGETMTVMMVTGNAPVLALKLGSLFSPVRTMTATIAAEMGEVAN
                     GSVHYHTLFLIGIVLFLISLIVNVVASSVVFRAKKRAERILS"
     gene            781..1644
                     /gene="pstA"
                     /locus_tag="PKI33_07230"
     CDS             781..1644
                     /gene="pstA"
                     /locus_tag="PKI33_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNM36835.1"
                     /translation="MMKMLARNRHTVQRIGYALITLMAVVTVFPIVGTVIFILFKGGS
                     AISWEFLTGFPHDGMRAGGILPAIIGTLYLTIGTAIFSVPLGIAAAIYLSEYAKDNQV
                     TRLIRLAIINLAGIPSVVYGLFGLGLFVLFLQFGTSILAASLTLSIMTLPVIISTAEE
                     ALRSVPQQFRTVSISLGATRWQTIRKIVLKEALPGILTGVILGLERAAGETAPILFTG
                     AAFFLPRLPQSPFDATMALPYHLFVISTQVPEMPIQIQYGTALVLLVFVLTMNVIATI
                     IRSRARAKRQW"
     gene            1656..2411
                     /locus_tag="PKI33_07235"
     CDS             1656..2411
                     /locus_tag="PKI33_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNM36836.1"
                     /translation="MNKIEIENLSLQYADGAESLRGISMGIRQNAVTVLFGPAGGGKS
                     TLLRCLNRLNDLTDIRTSSGRILIDGENILDPKTDVIALRRKVGMVFARPVPLPMSIR
                     ENITYGMELAGEKRRIKLDEAVERSLKLAAIWDEVKDRLNDPAVALSGGQQQRVCLAR
                     VLALQPEIILLDEPTSGLDPISTGKVEAALQELKKNYTVILVPHSVQQAARTADHAAF
                     FLQGELIEYDEGKRMFTTPKQKKTEDYIMGRFG"
     gene            complement(2532..>3200)
                     /locus_tag="PKI33_07240"
     CDS             complement(2532..>3200)
                     /locus_tag="PKI33_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNM36837.1"
                     /translation="DWWGGAGGGTVAHAAIASFLGDTAEAMMQFSHILPARIPRIALV
                     DFNNDSVRDSLRTLDAMFAKYRELMDAGKTEEAEKYKLYGIRLDTGGNLRDVSVTPLG
                     EPALDLGVNPRLVFNVRSALDSAWERWDLPTAWKEAAKEFCHSVKIVVSGGFAPEKIR
                     KFEKLDVPVDIYAVGSYLFSNGNGTSTDFTADVVRVKVHGEWIDMAKVGRKVGENENL
                     ERVW"
BASE COUNT          728 a          963 c          728 g          781 t
ORIGIN      
        1 cttgcctgcc ttaagtgaag tggaacccag cgcccttttt aacatacgtt ggtacccgat
       61 tgaaagctat tttggcattc tgccgctcat taccggttca ctcatcgtta ccctcggtgc
      121 agcacttatc gctgtacctt ttggaatcgg aaccgcaata tacatctccg aaattgcacc
      181 gcgttggatg cgcgaaattc tcaaacctct cgtggaattg ctcggcggac tcccttcggt
      241 agtgctggga tttctcggca tcctcgttgt ttcccccaac cttcgcatct tgctggacct
      301 gccaacgggt ctgaccgcat ttaccggatc cctcctcctc ggcgggatcg ccgtacccac
      361 ggtcgtgtct gttgcagaag atgcactcga tgccgtgccg cgttcttatc gtgaaggtgc
      421 atgggctttg ggtgccactc cctggcagac catctggcgt gtgacgcttc ccgcagcaaa
      481 atccggcgtg ctgaccgcca tcatgttggg agtgggtcgc gccatcggcg aaaccatgac
      541 cgtgatgatg gtcaccggca atgcgcccgt attggcactc aaactcggca gcctcttctc
      601 ccccgtccgc accatgaccg ccaccattgc cgcagagatg ggcgaagttg cgaatggaag
      661 cgttcactat cacactttgt tcttgatcgg catcgtcttg ttccttattt cattgatcgt
      721 gaatgttgtc gcatcctcag tcgtctttcg tgccaagaaa cgagcggaga ggattttgtc
      781 atgatgaaaa tgctcgctcg aaatcgtcac accgtgcagc gcattggcta cgctctcatc
      841 accctcatgg cagtggtgac cgttttcccc attgtgggca cggttatctt catcttattc
      901 aaaggtggct ctgcgatctc gtgggaattt ctcaccggtt ttccacacga tggcatgcgc
      961 gctggtggta ttttgcctgc catcatcggc acactctatc tgaccatcgg cactgccatc
     1021 ttctccgttc cgcttgggat cgccgccgcc atttatcttt ctgaatacgc caaggataat
     1081 caagtcacgc gccttattcg tttggcgatc atcaacctgg caggcatccc ttcggtggtg
     1141 tatggattgt tcggcttggg cttgttcgtg ctctttcttc agttcggcac ctccatcctc
     1201 gccgcctcat tgactctatc aatcatgacc ttgcccgtca tcatcagcac cgcagaagaa
     1261 gcgttgcgtt cggtgccgca acaatttcgc acagtgagca tctcccttgg cgccactcgt
     1321 tggcaaacca ttcgcaagat cgtattgaaa gaagcgttgc ccggcatcct cacgggagtc
     1381 attctcggct tggaacgtgc cgccggtgag actgcaccca tcctcttcac gggtgccgcg
     1441 ttcttcctcc cgcgcttgcc ccaatctcct ttcgatgcga ccatggcatt gccttatcat
     1501 ctctttgtca tttccaccca agtgcctgaa atgccgattc aaatccaata tggaacggcg
     1561 ttggtcttgc tcgtgtttgt cttaaccatg aacgtgatcg caaccatcat tcgttcgcgg
     1621 gcaagagcaa aacgtcagtg gtaatcaatc gtcaaatgaa caaaatcgaa atcgaaaacc
     1681 tctccctcca atacgccgat ggcgcagagt cattgcgcgg catcagcatg ggaatccgtc
     1741 agaatgcagt gaccgttctc ttcggtccgg caggaggcgg gaaatccacc ttgttgcgat
     1801 gtctcaatcg tctcaacgat ctgaccgaca tccgcaccag ttctggtcgc atcctcattg
     1861 atggcgaaaa tatcctcgac cccaaaacgg atgtgatcgc cctgcgccgt aaagtaggca
     1921 tggtgtttgc aagacctgtg ccgctcccca tgagcattcg tgagaacatc acctatggta
     1981 tggaacttgc cggagaaaaa cgccgcatca aactcgacga agcagtggaa cgcagtttga
     2041 aacttgccgc catttgggat gaagtcaaag accgcctcaa cgaccctgcc gttgcactct
     2101 ctggcgggca gcaacaacgc gtttgccttg cacgtgttct ggcactgcaa cctgaaatta
     2161 tcttgctcga tgaacccacc tcgggtcttg acccgatctc aactggcaag gttgaagccg
     2221 cattacaaga actgaaaaag aattacaccg ttatcctcgt cccccactct gtgcaacaag
     2281 ccgcccgtac tgccgaccac gccgccttct ttttacaagg ggaactgatc gaatacgatg
     2341 aagggaaaag aatgttcaca acccccaaac aaaagaaaac cgaagactac atcatgggac
     2401 ggtttggata gtaggggcaa cccacttgtg tctaaaatag ccaaaacaaa aagagcgggt
     2461 tgcccaaata tgggcgaccc gctcttttca taacaaggtt ctcaacactt ctggcgggtc
     2521 acccctacaa atcaccaaac ccgttccaaa ttctcgttct cccctacctt gcgtcccacc
     2581 ttcgccatat cgatccactc gccatggacc tttactcgca ccacatcggc ggtgaagtct
     2641 gtactggtac cattaccatt gctaaacaga tatgaaccca ctgcataaat atctacaggt
     2701 acatccagtt tctcgaactt gcggattttt tctggagcaa agccacccga gacgacgatc
     2761 ttcacactgt gacaaaattc cttcgccgct tctttccaag ccgtaggcag atcccaacgt
     2821 tcccaagctg aatccaacgc cgaacggacg ttgaatacca aacgcgggtt gactcccaag
     2881 tccaatgctg gctcacccaa cggagtgacc gatacatcgc gcaagttccc acccgtgtcc
     2941 aaacgaatgc cgtaaagttt atatttctca gcttcttcgg ttttcccagc atccatcaac
     3001 tcacgatact tggcaaacat cgcatccaac gtccgcaacg aatcgcggac cgagtcattg
     3061 ttgaaatcca ccaaagcaat gcgcggaata cgtgcaggca aaatatgcga gaattgcatc
     3121 attgcctcag ctgtatcgcc aaggaaggat gcaatcgcag catgagccac cgtcccaccg
     3181 cctgcgccgc cccaccaatc
//
LOCUS       DAOREF010000295         3190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19799_length_3190_cov_3.518979, whole genome shotgun sequence.
ACCESSION   DAOREF010000295 DAOREF010000000
VERSION     DAOREF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19799_length_3190_cov_3.518979"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1046)
                     /locus_tag="PKI33_07245"
     CDS             complement(123..1046)
                     /locus_tag="PKI33_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNM36838.1"
                     /translation="MQYYEFRAMNTTVTLGAESQYTTMAFQRAQDFIEKSEQRFSRFL
                     HNSELNALNRSAGTWFQASAEMMELITIALECHLATDRLFNPAILPDLQAAGYDRSMD
                     QLRLHGADVKPQRSPAPVLPFHNVQLDIDQNRILLPTNMQIDLGGIAKGWIAEKAATI
                     LAAYSSACVVNAGGDMFLIGHPNGQNWEVVLEDPLDQTQDVLMLLVEDGAVATSSTTK
                     RLWTQGEQVRHHLIDPRSGEPAKSPWLSVTVFAPHTSQAETFAKALLIAGPEGAQALL
                     NKSPEISFIAVDTERQIWKSPTEREKLYEYA"
     gene            complement(1046..1324)
                     /locus_tag="PKI33_07250"
     CDS             complement(1046..1324)
                     /locus_tag="PKI33_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36839.1"
                     /translation="MNLYRNLLKIWIGIVSLAGFVTGWVFIARASELETVTYVGNTAI
                     TMPELQPIPTVDGLAGEQLTTSNVQTFTVNTVVQQQQTFTQPMRTGGS"
     gene            complement(1324..1746)
                     /locus_tag="PKI33_07255"
     CDS             complement(1324..1746)
                     /locus_tag="PKI33_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36840.1"
                     /translation="MKKTIPAFIAALLITALLGGSMLLMGQNAVGVSTAKAEAAQTTQ
                     TSADTVAQYEQVIAQYQARETQYQDLVSQASIQITTANQQIELANQQLQQYQSLLQQL
                     QSSGLITIASDGTVTINQTSSSSAFMQPPQGHHGEGNH"
     gene            complement(1810..2142)
                     /locus_tag="PKI33_07260"
     CDS             complement(1810..2142)
                     /locus_tag="PKI33_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36841.1"
                     /translation="MKTILRILVILLIAGIVSGGVYALVENTSLMSGAEAERGAPPQM
                     TGTDGQTFQPMERPDGGGDRDSASLSRGFGEVGVLLAKITGITVVIVLIQKALGLFQK
                     NKPVIARA"
     gene            complement(2139..2666)
                     /locus_tag="PKI33_07265"
     CDS             complement(2139..2666)
                     /locus_tag="PKI33_07265"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNM36842.1"
                     /translation="MNVSTKTNLVLDITIFTAFLAIASPKLTGNTIHEWLAVAFGAAI
                     VTHLLFHWKWLVEVTTKFFKNFFSQSRLNYVVDLLFFLAMTATLFSGLMISKDVMSFF
                     GIQLNVSHSWESLHRLVSDVSVILLGIHFALHWKWIVTNVSRYIVSPVANLFKPQAQK
                     PVVSMTLQTSKEKKS"
BASE COUNT          843 a          710 c          755 g          882 t
ORIGIN      
        1 caaaaaagtc tttgaccgtt tgcgtctgca acgcgagtaa acctacagct agtaaacaca
       61 cgcccgtaac aaggacgccc accacggtaa agatatcgag ccagttttca gtttgaggtt
      121 ggttatgcgt actcataaag tttttctctt tcagtgggag atttccaaat ttgtctttcg
      181 gtatctacag caataaatga gatctctggg cttttattca ataacgcctg tgcgccttcg
      241 ggtccagcaa ttaagagggc tttagcaaag gtctctgctt gtgatgtatg tggagcaaag
      301 acagtgacgc tcagccaagg tgacttggca ggttcgcccg agcgtggatc gattagatgg
      361 tggcgaacct gctctccctg agtccagagg cgtttggttg tggaagatgt cgccacagcc
      421 ccatcctcta ccaagagcat caagacatct tgggtttggt caagcgggtc ttcgagcacc
      481 acttcccaat tctgcccgtt ggggtgaccg atgaggaaca tatctccgcc agcattgacc
      541 acacaagctg aagaataagc tgctaagatc gtggctgctt tttcagcaat ccagcctttg
      601 gcaataccac caaggtcaat ctgcatattc gttgggagaa gaatacgatt ctgatctatg
      661 tccaactgaa cattatgaaa gggcaaaacg ggtgcaggtg atctttgagg tttcacatca
      721 gcaccatgca aacgaagttg atccatacta cggtcgtagc cagccgcttg aaggtctggc
      781 aagattgccg ggttgaataa tctatctgtc gcaagatgac actccaacgc gatagttatc
      841 agttccatca tttcagccga agcttgaaac cacgtgccag cactacgatt aagagcattt
      901 aattcgctat tgtgcagaaa acgtgaaaag cgttgttcgc tcttttcaat aaaatcttgt
      961 gctcgttgaa acgccattgt ggtgtattgg ctttcagcgc ctaaagttac cgttgtgttc
     1021 atggcgcgaa attcatagta ttgcattatg aacctccggt acgcatgggt tgagtaaagg
     1081 tttgctgctg ttgtacaacc gtattgacgg taaaggtttg cacattagac gttgttagct
     1141 gttcgccagc caacccatcc acagttggaa tcggctgtag ttcaggcatg gtaatggcag
     1201 tattgccaac ataggtcaca gtttcaagtt cggaggcgcg ggcaataaaa acccagcccg
     1261 taacaaaacc tgccaaactt acaatgccga tccatatttt gagtagattg cgatataggt
     1321 tcattagtgg tttccttcac cgtgatgtcc ttgaggtggc tgcataaaag cggacgacga
     1381 gcttgtttga ttgatcgtca cggtgccatc actggctata gtgatcaagc cgcttgattg
     1441 caattgctgt aatagcgatt gatactgctg taactgttga ttcgccaatt caatttgctg
     1501 attggctgtt gtaatctgta ttgatgcctg gctgaccaaa tcttggtatt gtgtttctct
     1561 tgcctggtat tgcgcaatga cttgttcgta ttgtgcaacc gtatctgccg aagtttgagt
     1621 cgtttgtgcc gcttctgctt ttgcggtaga aacacccact gcgttctgcc ccatcagtag
     1681 catgctgcca ccaagtaaag cagtgatcaa caaggctgca ataaatgcgg gaatagtctt
     1741 tttcattagg ttctctctcc atgagacatc gcaagtctta cttgcgatgt ctcatatctg
     1801 ttttaagggt taggctcggg caataacagg tttattcttc tgaaacaagc ccagtgcttt
     1861 ttgaatgagc acgataacaa ctgtgatgcc agtgatcttg gctaacaaaa cacctacttc
     1921 accaaagccg cgggacaaag aggctgagtc acgatctccg ccgccatcag gtcgttccat
     1981 cggttggaag gtctgaccat ctgtaccggt catttgagga ggcgctccgc gctccgcttc
     2041 agccccagac atgaggctgg tgttttcaac caaggcgtaa actccgccgg atacgattcc
     2101 tgcaatcagc aggatgacaa ggattctcaa aatggttttc acgatttttt ctccttggag
     2161 gtttgcaggg tcatggatac aacaggtttc tgtgcctgcg gcttgaacaa gttcgccact
     2221 gggctaacaa tatagcgact gacattggta acaatccact tccagtgcaa ggcaaagtga
     2281 atgccgagta agatcacaga tacatctgaa acgaggcggt gcaggctttc ccagctatgg
     2341 ctcacgttca gttgaattcc gaagaaagac attacatctt tcgagatcat cagtccacta
     2401 aagagcgtgg cggtcatggc gaggaagaag agcaaatcca ctacatagtt gaggcgagac
     2461 tgactgaaaa aattcttgaa gaatttcgtg gtcacctcta cgagccactt ccagtggaaa
     2521 agtaaatggg tcacaatggc ggctccaaat gcgactgcca gccattcatg gatggtattg
     2581 ccggtcagct ttgggctggc aatcgccaaa aaggcggtaa agatcgtaat gtcgagtacg
     2641 agattggttt tagtcgatac gttcatgatt ttaggtttcc tttcgttgcc gcgaattttg
     2701 tttcgcgggg atgtttctat ttgatttatt cgaacacctg cattgtagaa ggcgaaactg
     2761 tgaaaaaacc aagaacggtt tgtataaaat ctgtaaagaa tgtgaatttc ctgtgaagac
     2821 tgggtataaa aagcaaaaag cgtcccccgt atcactacga gggacgctca actttcttat
     2881 aaaacttcgt ttacgctaaa gggaatccac ctcctttcag agatcgagac attgaagcga
     2941 gtaaggtcgc taggcactcc tttcttcaac cggtttggtt gaacaaagtc agtatagaaa
     3001 atcatcctga gaattagccg aaaaattcat aaacccctgc taagaactag atgtgaatct
     3061 ttctcatcta gttctgtttt gtccgtaaat atgcacgaga cgctcacaat tttcacagta
     3121 ttttcacagg gtcgtttttt atattgaaag agaaatatct gatctcattt ttacagggaa
     3181 attaagccca
//
LOCUS       DAOREF010000296         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19807_length_3189_cov_4.832482, whole genome shotgun sequence.
ACCESSION   DAOREF010000296 DAOREF010000000
VERSION     DAOREF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19807_length_3189_cov_4.832482"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKI33_07270"
     CDS             <1..272
                     /locus_tag="PKI33_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNM36843.1"
                     /translation="VILGEHMGQFLIILWMSMISGIMYKSKMFSKWVAILGWVASAIY
                     LLAQTELLATAIPTFPVIDWAGLVGSLLWLVWMIVMGVYLVKYKE"
     gene            274..1164
                     /locus_tag="PKI33_07275"
     CDS             274..1164
                     /locus_tag="PKI33_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNM36844.1"
                     /translation="MQFPEPFLSNIKHSFGEAGEIFLASLPALIKEASQRWQLTDIQP
                     VSNLSYNFVAFAQRGRDKVMLKIGVPHRELISEMAALILFNGDGACQIIDCDEGKGFL
                     LIEQLQPGKMLADLEDDDERTRIAIEVMKKIWRPAPETGKFIQLEDWFAELENIRPAF
                     DGTTGPYPKELLEKVESFLPELFKDEHYLIHGDFHHFNILSSERGWLAIDPKGVIGPC
                     GYEVGPLMLNPWNNSMDSAQFEVRAKRRVDLLHEGLGWDREQIIQWSTAHALLSSWWD
                     YPNGDWQCSLRCANIFAGLK"
     gene            complement(1206..1355)
                     /locus_tag="PKI33_07280"
     CDS             complement(1206..1355)
                     /locus_tag="PKI33_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36845.1"
                     /translation="MKVSLPKTISGWCMWLFFLCFGLATFVPVLGTITPWLALAYAVL
                     SLLGM"
     gene            1468..>3189
                     /locus_tag="PKI33_07285"
     CDS             1468..>3189
                     /locus_tag="PKI33_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36846.1"
                     /translation="MTSLSVPAEFTLQRKNKYDQSTTLRWILSHVQPYWWIVAMIFIG
                     AIGNAVLAVVIPVLTGDAFNAMLAPKPDTSVLLPLAVTIGISQIIRGVLQLGRNFGAE
                     LMAQKMERDIRDELYLSLLGKSMTFHNLQPVGDTMARSTNDVREVNYMFSPGVNLVVG
                     SFMFILMPLFFGPRYHPALVLTPLLFTIFYFIALVRYLKTLSPITDDVRATFGQMNTH
                     LSESLDGVEIMKGAAQEEAEVDRFVTNARKVRDAFVQQGDLEARYIAMLLLGLAFAGG
                     LVHALFLLRQGLINVGAVIGYFGMLQLLGFPTFTSTFAYSQISLGFSSARRILELIQR
                     ETKLDQNASGMTSEIRGEIEFRNVSFHYPTPAEGGAGGDNVLENISFKVKPGQTVALV
                     GQTGAGKTTLVKLINRIYDVTDGQVLVDGVDVREWNLASLRSQISIIEQDIFLFSRTL
                     SDNIAFGKPGATKEEIIAASMAAQAHDFILDFDETYATVVGERGVTLSGGQRQRIALA
                     RAFLTEPHVLVLDDSTSAIDSATEDKIQRAISNAAKGRTTFIITHRLSQIRWADLIIV
                     LRKGKVVA"
BASE COUNT          772 a          797 c          777 g          843 t
ORIGIN      
        1 gagtcatcct cggcgaacat atgggacaat tccttatcat cctgtggatg tctatgatca
       61 gcggcattat gtacaaatcc aagatgttta gcaaatgggt tgccattctg ggatgggttg
      121 cctctgccat ttacctcctc gcgcagacgg aacttctcgc caccgctatt cccactttcc
      181 ccgttatcga ttgggcggga ctggttggca gcttactttg gctggtctgg atgatcgtga
      241 tgggagtata tttggtgaaa tataaggaat agtatgcaat tccctgaacc ctttctctca
      301 aacatcaaac attcttttgg cgaggctggc gagatatttc ttgccagcct ccctgctttg
      361 atcaaagaag catcgcaacg ctggcagttg accgacattc agcctgtgag caatctctcg
      421 tataacttcg tcgcctttgc ccagcgtgga cgtgataaag tgatgctcaa gatcggtgtg
      481 ccacaccgcg aactaatcag cgaaatggca gcgctgatat tgttcaacgg tgatggagcc
      541 tgtcagatca ttgattgtga tgaaggaaag ggatttttat taattgaaca acttcagcca
      601 ggcaaaatgc tggcagactt ggaagatgat gacgagcgga ctcgcattgc aatagaagtc
      661 atgaaaaaaa tctggcgccc tgcacctgaa accggaaagt ttattcagtt ggaagattgg
      721 tttgcagagt tagaaaacat ccgccccgct ttcgatggaa caaccggacc ataccctaaa
      781 gaattgttgg agaaggttga atcatttcta ccggaattat tcaaagatga gcattatttg
      841 attcatggtg attttcatca tttcaacatt ctctcttcag aacgtggttg gctcgccatt
      901 gaccctaagg gtgtgattgg tccttgtggc tatgaggtcg gtccgctcat gctcaacccg
      961 tggaataact caatggacag tgcccagttc gaggtccgtg caaagaggcg ggtggacttg
     1021 ctccatgaag ggttaggctg ggatcgcgag cagatcatcc aatggtccac agcccatgcc
     1081 ctcttatcct cctggtggga ttatcccaat ggcgattggc aatgctcgtt acggtgtgcc
     1141 aatatttttg caggacttaa ataacacaac ctccctttcg ggaggttgtg tgtttataaa
     1201 ttgagttaca taccaagaag gcttaagaca gcgtaggcaa gagccagcca gggagtgatg
     1261 gtgcctaata cgggcacgaa tgttgcgaga ccaaagcaca agaagaacag ccacatacac
     1321 caaccagaaa tcgttttggg caaagaaact ttcatttcac actctcctat aaattgttgg
     1381 cgctggcaga attgccagct acaaaataaa acaccttact aaagtgaggg ttgcggtgct
     1441 cttttttagg gatgttacaa ttactttatg acttccctaa gtgttccagc agaatttact
     1501 ttacagagaa agaataagta cgatcaatcc acaacattgc gatggattct ttctcatgta
     1561 cagccttatt ggtggatcgt agccatgatc ttcattggtg caattggcaa cgccgtattg
     1621 gctgttgtca ttccggtgtt gaccggtgat gcattcaatg ccatgcttgc ccccaagcca
     1681 gatacctctg tgcttctgcc gttggctgta acgatcggca tttcacagat catccgtggg
     1741 gtgctgcaat tggggcgtaa ctttggtgcc gagttgatgg cacaaaaaat ggaacgagac
     1801 atccgcgatg agttgtatct ctcgctgctt ggcaagagta tgaccttcca taacttacaa
     1861 cctgtgggcg atacaatggc acgctctacg aacgatgtgc gcgaagtgaa ttacatgttt
     1921 agccccggcg tgaatcttgt ggtcggttcg ttcatgttca tcctcatgcc actgttcttt
     1981 ggtccgcgct atcatccagc gttggtactc accccgctgc tatttaccat tttctatttc
     2041 atcgcattgg ttcgatatct caaaacgctt tcgcctatta ccgatgatgt gcgtgcaacg
     2101 tttgggcaga tgaacacaca tctatcggag tcacttgatg gggtcgagat catgaaaggt
     2161 gcggcgcaag aagaagccga agtggaccgt ttcgtgacca acgcccgcaa agttcgtgat
     2221 gcttttgtgc agcaaggtga ccttgaagcg cgttacattg ccatgctctt gctcggtctg
     2281 gctttcgcag gcgggcttgt ccacgcattg tttttgctgc gccaagggct aatcaacgtt
     2341 ggagccgtga ttggctattt tggcatgctg caactcttgg gctttcccac tttcacttca
     2401 acctttgcct actctcaaat ctctttggga ttttcatccg ctcgccgtat cttggagctc
     2461 attcaacgcg agacaaaact tgaccaaaac gcctcaggca tgacctcgga gattcgcggc
     2521 gaaattgagt ttcgcaacgt ctcgttccac taccctacac ctgctgaagg cggtgccggt
     2581 ggagataatg tacttgagaa tatttcattc aaagtgaaac ccggtcaaac cgtggcactc
     2641 gtcgggcaga ccggtgctgg caagaccacc ctcgtcaagc tcatcaaccg catctatgat
     2701 gtgacagatg ggcaagtgct tgttgatggt gtagacgtgc gcgaatggaa tctggcatcg
     2761 ctgcgttctc agatatccat cattgaacaa gatatcttcc tcttctcgcg cacccttagc
     2821 gataatatcg cctttggcaa acccggtgcg accaaagaag aaatcattgc tgcatccatg
     2881 gcagctcaag cgcacgactt cattttggac tttgacgaaa cctatgcaac cgtggtcggc
     2941 gaacgcggtg tgactctctc cggtgggcaa cgtcaacgca tcgcgctggc tcgtgccttc
     3001 ctcaccgagc cgcacgtgct cgtcctcgac gactcaacgt ccgcgattga ctcagcgacg
     3061 gaagacaaaa ttcaacgcgc catctccaac gccgctaaag gacgcaccac cttcatcatc
     3121 acccaccgtc tctcccaaat ccgctgggca gacctcatca tcgtgctccg caaaggcaaa
     3181 gtcgttgcg
//
LOCUS       DAOREF010000297         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19824_length_3187_cov_4.769796, whole genome shotgun sequence.
ACCESSION   DAOREF010000297 DAOREF010000000
VERSION     DAOREF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19824_length_3187_cov_4.769796"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKI33_07290"
     CDS             <1..470
                     /locus_tag="PKI33_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM36847.1"
                     /translation="AIYLLPVNLYGPRDNFNLQTSHVIPALIRKAVEAGERGDKELPA
                     WGDGSPTREFLYVEDAADGIVTAAENYNGDLPVNLGSGYEISIKDLTEMVAKLTDFQG
                     KIVWQTDKPNGQPRRGLDVSRAKELFNWSAQVSFEEGMKRTIEWFKANRDKIK"
     gene            473..1318
                     /locus_tag="PKI33_07295"
     CDS             473..1318
                     /locus_tag="PKI33_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36848.1"
                     /translation="MTELTKHEPHTVYIKPSKGLAALNLRDLWVYRELVYFMVWRDVK
                     VKYKQTLLGMAWAVVQPVMTMLVFTFLFGKVAKLPTDGIPYPVFSFTALLPWGLFVNA
                     LNQGSRSLVAHNNMVTKIYFPRLILPMASVFSGMVDFVIAFVILVGLMFYYQVTPAWN
                     LLWTLPLFLLLALVTALGVALWLSAINVQYRDVNQALPFLSQFWLFATPVAYSSKVIS
                     EKWQVVYSLNPMAGVVNGFRWALLGTGNGPDISLWVSVGISILVFVTGLFYFKSMEKT
                     FADTI"
     gene            1318..2607
                     /locus_tag="PKI33_07300"
     CDS             1318..2607
                     /locus_tag="PKI33_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36849.1"
                     /translation="MATAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVAAPVRLAK
                     AMIGKSDRRLNQNYVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTDPTTGS
                     VTVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMKRSEIESKFDEIVDFSEVSQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRT
                     VLFVSHNMSAILRLTQEAVVLNKGQLIMRGPTQEAVDYYLSSGQSQAGERTWDADEVP
                     ATSAPFMPISLKVKERSGKIVDTIRSTEKVTVEFEYKLSSPVTGLRVGLYISTMRGEY
                     VFTSFDTDTPELYEKFDSRNAGHYISRAELPADIFNEGRYMVGVNASSFGVRRYFMDE
                     NALAFNVDISGAPGTQWGEPRVGPIRPRLNWKIEKID"
     gene            2646..>3187
                     /locus_tag="PKI33_07305"
     CDS             2646..>3187
                     /locus_tag="PKI33_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36850.1"
                     /translation="MPKPNPLREILGDLPFTAEIDWMLRSKNRPRKDHYNLTRLLTSL
                     PDAVKVVKPYAESAPRGKNVLFFATLHYWIEQSAYLGLVLAGMGHDVTLLTLPYSEWH
                     KEKDKFTQRQRILHTHDALKMLSPLVKHISMLDMQSSSRASHGTLHAHAASELPEKIQ
                     TDIKEISLWDVQYTLMREEV"
BASE COUNT          759 a          738 c          809 g          881 t
ORIGIN      
        1 atgccattta tcttttgcca gtgaacctct atggtccgcg cgataacttt aatttgcaga
       61 cttcacatgt catccctgcg cttattcgca aggcagtaga agctggcgaa cgtggtgaca
      121 aagaactccc cgcttggggc gatggttcac ccactcgtga atttctctat gtggaagatg
      181 ccgccgacgg cattgtgact gctgccgaaa actacaacgg cgatttgccg gtcaaccttg
      241 gttctgggta tgaaatttct atcaaagacc tcaccgagat ggttgcaaaa ctcaccgact
      301 tccaaggcaa aatcgtctgg cagacagata agccaaatgg tcagccccgc cgcggacttg
      361 acgtctcgcg ggcaaaagaa ctcttcaact ggagcgcaca agtttcattt gaagaaggca
      421 tgaagcgcac catcgaatgg ttcaaagcca atcgcgataa gatcaagtaa atatgactga
      481 acttacaaaa cacgaacctc atactgttta tatcaagccg tctaaagggc ttgcggcact
      541 caacttgcga gacctttggg tgtatcgcga attggtttat ttcatggttt ggcgtgatgt
      601 caaagtgaaa tacaagcaga cgttgcttgg tatggcttgg gcagttgtcc aaccggtgat
      661 gacgatgttg gtcttcactt tcctctttgg aaaagtcgct aaattaccta cagacggcat
      721 tccttacccg gtcttttcgt ttaccgccct tttgccgtgg ggactattcg tcaacgcact
      781 taaccaaggc agccgttcgc ttgtggcgca caataacatg gtgacgaaga tttattttcc
      841 gcgtctgatt ctgccgatgg cttctgtctt ttcgggcatg gtggattttg tgattgcctt
      901 tgtcatcttg gttggcttga tgttctacta tcaagttacc cctgcctgga atttactctg
      961 gacgcttcca ctcttcctct tgcttgctct tgtgaccgca cttggagtgg cgctttggct
     1021 ttctgctatt aacgttcaat atcgtgacgt gaatcaggcg cttcccttcc tttcgcagtt
     1081 ctggttgttt gctacaccag tggcatattc ctccaaggtc atttcagaga agtggcaggt
     1141 tgtctattcc ctaaacccga tggcgggtgt ggtcaacggc ttccgatggg cattgctcgg
     1201 tacgggcaat ggaccggata tctcgttgtg ggtttctgtt ggaatttcaa ttttggtttt
     1261 tgtgacgggt ctcttctact tcaaaagtat ggaaaagacc tttgcggata cgatctaatg
     1321 gcaacagcga tttcagtaaa aaatattggt aagcaataca agatcggcgc ggcggaaacc
     1381 aaattccgct ataacatgtt gcgtgatgtg atcgtggata cggttgcagc gcccgtgcgt
     1441 ttggcgaagg ctatgatcgg aaagtctgac cgccgcctga accagaatta tgtctgggca
     1501 ttgaaagatg tttcctttga cctcgaagaa ggcaaagtgc ttggcattgt ggggcgtaac
     1561 ggtgctggta aatctacctt gctgaaaatc ctttcccgcg tcactgatcc gaccactggt
     1621 tctgtcaccg tgcgcggacg ggttggctcc ctgcttgagg taggaacggg ctttcaccct
     1681 gaattgaccg ggcgtgagaa tatctacatg aacggcgcca tcctcggtat gaaacgctct
     1741 gagatcgaat caaagttcga tgagatcgtg gatttctctg aagtgagcca gttcatcgat
     1801 acacctgtga agcgatattc atcgggtatg tacttgcgtt tggcatttgc cgtagcggcg
     1861 catctcgaac ctgaaattct tgtggtggat gaagtgcttg ctgtaggcga tgccgaattc
     1921 caaaagaaat gtcttggaaa gatgggtgat gttgctcagc agggacgcac tgttttgttc
     1981 gtcagccata atatgtctgc gattttgcgc cttacgcaag aagcggttgt gttgaacaag
     2041 ggtcaactta tcatgcgtgg acctactcaa gaggcggtag attactacct ctcctcggga
     2101 caatctcaag ccggagaacg cacttgggat gccgatgagg ttccagcgac gagtgcgcct
     2161 tttatgccga tcagcctcaa agttaaagaa agaagcggaa agatcgtcga taccatccgc
     2221 tctacagaaa aagtcacggt tgagtttgag tacaagttat cctcgcctgt gactggtttg
     2281 cgtgtgggtc tgtatattag caccatgcgc ggcgagtatg tgtttacttc attcgatacc
     2341 gacacccctg aactttatga aaaatttgac tcgcgtaatg cgggtcatta catcagccgc
     2401 gcggaattgc ccgccgatat tttcaacgaa ggtcgttata tggttggcgt gaatgcgagc
     2461 tcatttggtg tgcgccgcta tttcatggac gagaatgcgt tggcgttcaa tgtggatatt
     2521 tctggcgctc ctggtacgca atggggcgag ccgcgtgtgg gaccgatacg cccacgattg
     2581 aattggaaga ttgagaagat agattaatcg caggtcacct tacaaaaatg accttttgaa
     2641 aattcatgcc aaaacctaac ccactccgcg aaattttggg cgacctgccc ttcactgccg
     2701 aaatcgattg gatgcttcgc tcgaagaatc gtccgcgcaa ggatcactac aacctgacgc
     2761 gcttgctcac atccttgcca gatgccgtga aggtggtaaa gccttatgcc gagagcgcac
     2821 cgcgtggaaa aaatgttttg ttctttgcca cgcttcacta ctggatcgaa caatcggcat
     2881 atttgggttt ggtgttggca ggcatggggc atgatgtcac tttgttgact ttgccctatt
     2941 ctgaatggca taaagaaaaa gacaaattta cccaacgtca gcgcatcctt catacgcatg
     3001 acgcattgaa aatgttatct ccgctcgtga agcatatttc tatgttagac atgcaatctt
     3061 catcgcgtgc ttctcatggg acgttgcatg ctcatgctgc aagtgaattg cctgaaaaaa
     3121 ttcaaaccga tatcaaagag atttcccttt gggatgtgca atacactttg atgcgcgaag
     3181 aagtgga
//
LOCUS       DAOREF010000298         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19825_length_3187_cov_4.072158, whole genome shotgun sequence.
ACCESSION   DAOREF010000298 DAOREF010000000
VERSION     DAOREF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19825_length_3187_cov_4.072158"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="PKI33_07310"
     CDS             complement(<1..1567)
                     /locus_tag="PKI33_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNM36851.1"
                     /translation="MPLLKPSAPQSNSKKPAGKTLPPKKGGADLPSKAPAKGRGLPPQ
                     APPSPPPSYTPQPLREQTWWEKLSPERKLDVVGVILAFFGLVILLGLLSANRSAIVGG
                     AIFFLAQLFGWGVYVLPIGLLVFGLWLVFRKIERIPPLSTERAVGSVILFFWLMTLLH
                     SFIATAETAEAVALTGQGGGYFGGLAQRILWGAFGAGGAFIAMIAWLIIGVAVLLDKP
                     VIELFFWLKPLMLKLRDLIIKPAPQTQPVTAQPQSSDGLTPIDSTAMPAMDMTNAVPV
                     QPVQTRSGTTIIEWKLPDLTDILDEGSEATVNEDFIKQRAHLIEETLASFGAPAQVVA
                     ISRGPSITQFGVEPLFLESRGGVRTRVRVSKIASLADDLALALAAPRIRIQAPVPGHS
                     YVGIEVPNDEMARVALRDILEGEIFKGYKKPLAFALGRDVAGLPAISSIENMPHLLIA
                     GTTGSGKSVCVNSILTCMLLYNTPDDLRLVLVDPKRVELTGYNGVPHLLGPVVVEMDR
                     VIGALQWITREMDK"
     gene            1843..2763
                     /locus_tag="PKI33_07315"
     CDS             1843..2763
                     /locus_tag="PKI33_07315"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNM36852.1"
                     /translation="MFEILFLGTSASAPSAHRGLSSHIVSHNEYRFLVDCGEGTQRQI
                     LQSGKGFKRLTRALITHGHLDHILGLGGLLSTFLRWEAIEEFEVYGSKGTLERVKTLL
                     FDVVLRGKKTPMPLRLIEIKPGVIFDAEDFTVTAFSVSHGKADSLGYIFEEKARRPFL
                     AEKADALGVPFGPERKALVNGEAITLADGTHISPEEVLGDWEKGSKLVVVGDAGRTDN
                     LIEACQDADAVVIESTYLDEEADMASQFSHLTAKMGAELAQKAGVKKLILTHISRRYR
                     GRDVLAEAQTVFPNTVVARDFDTFVIKKDN"
     gene            2765..>3187
                     /locus_tag="PKI33_07320"
     CDS             2765..>3187
                     /locus_tag="PKI33_07320"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNM36853.1"
                     /translation="MADKLTIGVLTSGGDAPGMNAAIRAVVRTALANNMNVIGVMHGY
                     EGLVNGEFKPMGARDVGGILQRGGTVLLTSRSKRFQEPTGQREAIRKMNEAGMDALIV
                     IGGEGSMNGANALSQQGVKVIGIPGSIDNDIWGTNIAIG"
BASE COUNT          805 a          714 c          875 g          793 t
ORIGIN      
        1 gcttgtccat ttcgcgggta atccattgca aggccccgat cacacgatcc atttcaacga
       61 ctacgggccc aagcagatgc ggaacgccgt tatacccggt caactccacg cgcttcgggt
      121 ctacaagcac aaggcgcaag tcgtcgggag tattgtataa aagcatacaa gtcagaatcg
      181 agttcacaca caccgactta ccagaacccg tcgtacccgc gattaacaag tgcggcatat
      241 tctcaatgct agagatggca ggcaacccag ccacgtcacg cccaagcgca aacgccaaag
      301 gttttttata ccctttgaaa atttcacctt ctaaaatatc gcgcaacgct acacgcgcca
      361 tttcgtcatt cggcacttcg atgccaacat aactatgccc cggtaccggt gcttgaatac
      421 ggatgcgcgg cgcggctagt gcaagggcga ggtcatcagc taaggatgcg atcttactta
      481 cgcggactct ggtccgtacg ccgcctcggg actcaagaaa tagcggctcc accccaaatt
      541 gcgtgatcga aggtccacgg ctgattgcca ctacttgcgc tggcgcgcca aaggaagcga
      601 gcgtctcttc aatgagatgt gcgcgttgct taatgaaatc ttcgttgacg gttgcttcac
      661 tgccttcatc aagaatatct gtcaggtctg gcaatttcca ttcgatgatg gttgtgccac
      721 tgcgagtttg aacaggttgt acggggactg cattcgtcat gtccatggcg ggcatggcag
      781 tagaatctat cggcgtgagt ccatcacttg attggggctg agcagtgacc ggttgagtct
      841 gaggcgcggg tttgataatc aggtcgcgca gtttcagcat caacggtttg agccagaaga
      901 aaagttcgat gacgggtttg tctagcaaaa cggcgacgcc aattatgagc caggcgatca
      961 tggcgatgaa ggcaccgcct gcgccaaagg ctccccaaag aatgcgttgc gccaaaccgc
     1021 cgaaataccc gccaccttga ccagtgagtg caaccgcttc agcagtttca gctgtggcga
     1081 tgaaggagtg caacagggtc atcagccaga agaaaaggat gacactccca actgcgcgtt
     1141 ctgtagaaag cggagggata cgttcgatct tacggaagac cagccacaat ccaaagacga
     1201 gcaagccaat gggtaagacg tatacgcccc agccaaatag ttgtgcaagg aaaaagatcg
     1261 cgccaccaac gatagcggag cggttggcag aaagaagccc taataagatg acaagaccaa
     1321 aaaatgcgag gatgacacct acaacatcta acttgcgttc gggtgaaagt ttttcccacc
     1381 aggtttgttc gcgcaggggt tggggtgtat atgatggagg tggggatggt ggagcttgag
     1441 gaggaagccc tctgcctttt gccggagctt tggaaggtaa atccgcgccg ccttttttgg
     1501 ggggaagagt tttgcccgcg ggctttttac tattcgattg aggcgcggag ggttttagga
     1561 gaggcattcg attttcaaga ggaaataggt tcgttcatca tttacgataa aaaatcgttc
     1621 ttgagtcatt atagcacaaa tggtctgttt aacttcttgt gctgattcta ttgagtttca
     1681 tttcaaacgt attcttcatt aaatgggttt ttgcccaata gttgatttga gttaaggtaa
     1741 aattgcaggg cttgttgcca gtttggttgt gtgcgataag agtatttttc agcgattgat
     1801 tcgcggggct acatttcctc cgcagtttga taggacacaa ttttgttcga aattctattt
     1861 cttggtacat ccgcatctgc gccatcggca caccgcgggc tttcttctca tatcgtctct
     1921 cataacgaat atcgtttttt ggtcgattgt ggcgaaggca cccagcgcca gatattacag
     1981 tctggcaagg gctttaagcg gttaacacgt gcgctcatca cccacggaca tctggatcat
     2041 attctggggt tgggtgggtt gctttccact tttttgcgtt gggaagcgat tgaagagttt
     2101 gaagtgtatg gcagtaaggg aacgcttgaa cgtgtaaaga ctttgttgtt cgatgtggtg
     2161 ttgcgcggca agaaaacgcc catgcctttg cgtttgatcg agataaaacc cggtgtgatc
     2221 ttcgatgcag aagatttcac tgtaactgct ttttctgttt ctcatggcaa ggcagatagc
     2281 cttggctata tctttgaaga aaaagcacgc cgtcctttcc ttgcagaaaa agcagatgct
     2341 ctaggtgtgc cgttcggacc tgagcgaaaa gctttggtga atggcgaggc gattacactt
     2401 gctgatggaa cacatatctc tccagaagaa gtgcttgggg attgggaaaa aggcagtaaa
     2461 ctggttgttg tgggtgatgc aggtagaact gataacttga tcgaagcttg tcaggatgcc
     2521 gatgcagttg tgattgagtc tacctatctg gatgaagaag cggatatggc aagtcagttt
     2581 tcgcatttga ccgcgaaaat gggtgcggaa ctcgcacaaa aagcaggggt taaaaaacta
     2641 atacttacgc atatttcacg acgctatcgt ggaagggatg tgcttgcaga agcacagact
     2701 gtttttccaa atacagtagt ggctcgtgat tttgatactt ttgtgataaa gaaagataat
     2761 tgagatggca gataaactta caattggtgt tttgacttcc ggaggcgatg cccccggcat
     2821 gaatgcggcg atccgcgcag ttgtgcgtac ggcgttagca aacaatatga acgtaatcgg
     2881 tgtgatgcac gggtatgaag gactagtcaa cggcgagttc aaaccgatgg gtgcgcgcga
     2941 tgtgggcggt atccttcagc gcggtgggac agtgctcctc accagccgca gcaaacgctt
     3001 ccaggaaccc actggtcagc gtgaagcgat ccgcaagatg aatgaagcag gcatggatgc
     3061 gttgatcgtc atcggcggcg aaggttcaat gaacggcgcg aacgcgcttt cacaacaagg
     3121 tgtgaaggtt attggcattc ccgggagtat cgacaacgat atatggggca cgaacattgc
     3181 gattggt
//
LOCUS       DAOREF010000299         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19837_length_3186_cov_4.739700, whole genome shotgun sequence.
ACCESSION   DAOREF010000299 DAOREF010000000
VERSION     DAOREF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19837_length_3186_cov_4.739700"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /gene="nusA"
                     /locus_tag="PKI33_07325"
     CDS             complement(<1..1645)
                     /gene="nusA"
                     /locus_tag="PKI33_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNM36854.1"
                     /translation="MAKNDFALAFNEVLEEKQLAKDVVITAIESAMVSAYRRAVGAST
                     AQYVESKLDPETGIVTVYAEKEVVEDSIVDDRTEVLLEVARKVNPECQPGDMVVVEST
                     PADFGRVAAQTARQVIQQRIREAERSNQMEYYQRQEGEIVSGLVQAINAQGITIGLDM
                     KAEGVMPNNQRIPGEKLKLHDRVRAVVMEVKDGTRGPQIILSRAHRNFLRRLMENEVP
                     EIYHGIVEIRAIAREPGARAKVAVSATQPGIDPVGACVGQKGVRIQAIVKELHDEKID
                     IILWDADPVAYISKAISPAKVTGVYLTEEERTATVVVGEDQLSLAIGRDGQNARLAAK
                     LTSWRIDIKSLSEAAGDSLAKLQGDAELQNMLKAAVEVMPQVEEILAKKAENRLITPE
                     EFSTLEQFVDRVERRMILAKEEASREEDERIAAARAEIPAAAFNMPLDQATNLKEHVF
                     NILTEAGYETVGQLIFDMKTDQNKVLGLAGIGGKAITNIEETLAALTFPEPVAEPKVE
                     ETPAVVAEPVVTETAPVVETPVVEKPAEKKKEPKRVIEEE"
     gene            1839..2573
                     /locus_tag="PKI33_07330"
     CDS             1839..2573
                     /locus_tag="PKI33_07330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36855.1"
                     /translation="MTERDMNKAALRGEPSYVWRDGQKRRLNMIAKFAGERMEGKVLE
                     NGCGVGMYVEKMTELGATVTGLEYDFERACDAHVNSPRIVNAAGEFLPLPSSTFDLIL
                     SHEVIEHVQDDRSAIAEMVRVMKVGGRAVIFCPNRGYPVETHGIFWNGKYYFGNKLFV
                     NYLPRVWRDKLAPHVRVYSRRDMQKLFEGLPVKFIERTIIFGAYDNIIARLGVFGKFL
                     RAVLQFMEATPLKILGLSHLWVVEKI"
     gene            complement(2608..3033)
                     /locus_tag="PKI33_07335"
     CDS             complement(2608..3033)
                     /locus_tag="PKI33_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36856.1"
                     /translation="MPQPLFAGLIVDEDGNPVQAASIGSEPAYVIDDAGFKRHIPAEQ
                     VDRAVLNQMADMMKGSEDVLSEQTAKMLGQEDPFSKAMIANQLKNIDKQFDALMKTGF
                     PEELRAYLGMMGFKIVINYHGEVVRVEQPGTSGGDEGDE"
BASE COUNT          711 a          854 c          807 g          814 t
ORIGIN      
        1 cctcttcttc gatgacgcgt ttcggttctt tcttcttttc ggcgggcttc tccacaacag
       61 gcgtttccac cactggagct gtttctgtca caacgggttc tgcaacaaca gccggggttt
      121 cctccacctt gggttcagca accggttcgg ggaaggtgag agccgcgagc gtttcttcga
      181 tgttcgtaat ggctttacca ccaataccag ccagccccaa cactttgttc tggtcggtct
      241 tcatatcgaa gatcaactga ccaacggttt catagccagc ttcagtaaga atgttgaaga
      301 cgtgttcctt gaggttggtt gcctgatcga gcggcatgtt gaacgcagcg gcagggatct
      361 cggcgcgagc ggcggcaatg cgttcgtctt cttcgcgaga agcctcttcc ttggcaagga
      421 tcatgcggcg ttccacacgg tccacgaatt gttcgagtgt gctgaattct tccggcgtaa
      481 tgaggcgatt ttcagccttc ttagcgagaa tttcttccac ctgcggcatc acttccaccg
      541 cagccttgag catattctgc aattcggcat cgccctgaag tttggcaagc gagtcgcctg
      601 cagcttcaga caaagacttg atgtcgatgc gccagctggt cagtttggca gcgaggcggg
      661 cattttgtcc atcacgaccg atggcaagac tcaactggtc ttcgccaact acaacggtag
      721 ctgtgcgttc ttcttcggtg agatatacgc cagtcacttt ggcggggcta atggctttgg
      781 agatgtaggc aaccgggtcg gcgtcccaca aaatgatgtc gatcttctca tcgtgcaatt
      841 ctttgacgat ggcttgaata cgaacaccct tttgacccac acatgcgccg acggggtcga
      901 taccgggttg cgtggcagaa accgccactt tggcgcgggc acctggttcg cgggcaatcg
      961 cgcggatctc aacaatgcca tgatagatct caggcacttc gttttccatc aagcggcgca
     1021 agaagttacg atgcgcgcgt gaaaggatga tctgcggacc acgggtcccg tctttgactt
     1081 ccatcaccac cgcacggacg cggtcgtgta gcttcaattt ttcaccagga atgcgctgat
     1141 tattcggcat cacgccttcg gctttcatat ccaagccgat agttatgccc tgtgcattga
     1201 tcgcctgtac caaaccagag acgatttcgc cttcctggcg ctggtaatac tccatctgat
     1261 tggagcgttc tgcttcacga atacgctgct ggatgacctg acgagccgtc tgtgccgcca
     1321 cacgaccgaa atcagccggg gtgctttcca ccacgaccat atcgcccggc tggcactcag
     1381 ggttcacctt gcgggctacc tcgagaagaa cctcagtgcg atcatcgacg atgctatctt
     1441 ctacaacttc cttctcagca tagacggtta caatccccgt ttcggggtcc agcttggact
     1501 ccacatattg agcggtggat gcccccacag cccgccggta tgcggaaacc atcgccgact
     1561 cgatcgcggt gatgaccacg tccttggcga gttgcttttc ttccagcact tcgttaaatg
     1621 ctagcgcaaa atcattcttt gccatcgtac tgctccaaaa ctcctttaga tatcataccc
     1681 atagggtata aagcgaagaa gtgggggctg cccacttctc ggtgacgttc actattcggc
     1741 ttgtccctta cgaaagggat tctatagcag aaaggtttca gcgtcaaggc tgagtctgtt
     1801 aaaggagcat ttgaacttgg cagtgtataa tcgccgccat gaccgaacga gatatgaaca
     1861 aagccgccct gcgcggagaa ccttcgtatg tatggcgcga tggacaaaag cgccgtttga
     1921 acatgattgc aaagtttgcc ggggaacgca tggaaggcaa agtgttggaa aatggctgcg
     1981 gcgtgggcat gtacgtagaa aagatgaccg aacttggggc aacagtcacc gggttggaat
     2041 atgactttga acgcgcctgt gatgcccatg tgaattcgcc gcgtatcgtc aacgccgccg
     2101 gggaattcct cccgcttcct tcttcgacgt ttgacctgat tctcagccac gaagtgatcg
     2161 aacacgtaca ggatgaccgc tccgcgattg ccgagatggt acgcgtgatg aaagtgggtg
     2221 ggcgggctgt catcttctgc cccaaccgcg gataccccgt agaaacccac ggcattttct
     2281 ggaatggcaa atattatttt ggtaataagc tgtttgtaaa ttacctaccc agagtgtggc
     2341 gcgacaaact cgccccgcac gtgcgcgtgt attcccgccg tgatatgcaa aaactatttg
     2401 agggattacc cgtcaaattc attgaacgca ccattatctt tggcgcctac gataacatca
     2461 tcgcccgctt gggcgtcttc ggaaaatttc tccgtgctgt gttgcaattt atggaagcga
     2521 caccgctaaa gatattgggt ttatcccatc tttgggtggt agagaagata taaaaaactc
     2581 cgaggttcaa cacctcggag tttttcttta ttcatcaccc tcatcgccac cggaagtccc
     2641 cggctgttcc actcgtacca cttcgccgtg gtaattgata actatcttga aacccatcat
     2701 ccctaaatag gcacgcagtt cttcaggaaa gcctgttttc atcaaggcat cgaattgctt
     2761 atctatattc tttagctgat tcgcgatcat cgcctttgag aagggatctt cctgcccaag
     2821 cattttggca gtctgctccg aaaggacatc ttcactgcct ttcatcatgt ctgccatttg
     2881 gttgagcacg gcgcgatcca cttgctcggc agggatgtga cgtttgaatc cggcatcatc
     2941 tattacgtaa gcaggttcac tgccaatgga tgcggcttgt acggggttgc cgtcttcatc
     3001 tacgatgaga ccggcgaaga gaggttgggg cattaagaac tcctctttct tctttcctct
     3061 tttgttttgc aagaaagaag aaagaggaaa gatatcaaga aattattgtt ggcgaccttc
     3121 aactgcagca agcaaaatgc cagcggcaat gccaagacgg cggtcaacct gacccgccgt
     3181 atccat
//
LOCUS       DAOREF010000300         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19887_length_3181_cov_4.223289, whole genome shotgun sequence.
ACCESSION   DAOREF010000300 DAOREF010000000
VERSION     DAOREF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19887_length_3181_cov_4.223289"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKI33_07340"
     CDS             complement(<1..751)
                     /locus_tag="PKI33_07340"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36857.1"
                     /translation="MNRELAMTTKPILSVRNLSVTYPDNNGGLHALDNISFEVRPREF
                     VCFLGPSGSGKTTFLRVLAGILEPTAGNVNFMYHHQQKIGMVFQQANLMPWRTVIQNI
                     MLPLELEGIGDIKSQNKAREMIKLVGLKGFEDAYPRDLSGGMAQRVAIARALIHDPDL
                     LLLDEPFASLDALTRERMWTELSNIWQSQQKTVIMVTHSINESLFLADRVMVLTKRPG
                     KIKMDVEVDLPRPRTDDIRYTSHFGKLAKKLR"
     gene            complement(789..1766)
                     /locus_tag="PKI33_07345"
     CDS             complement(789..1766)
                     /locus_tag="PKI33_07345"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM36858.1"
                     /translation="MKKVVLLMLTLTVALMGCTSSQSTNAAGELRHIKLPMGYIPNIQ
                     YAPFYVAIDKGYFAEEGIEIEFDYSFETDGVALTGAGELPFAVASGEQVLLARSQGLP
                     VVYAFAWYQQFPISIISAPELNINEPADLRGKKIGLPGLFGANYIGLEALLFEGGVDP
                     SEVSMDAIGFNQVEAYASGNSDIVVGYAANEPIVLASQGFDLKEMRVADYVDLIANGI
                     VTNEDTIANDPELVRGMAKALARGIEATINNPEEAYDISLKFVENLKDQDKAVQMQVL
                     NTSIEFWKADRIGYSNPQAWENMNNVLVKMALIPEPIDLSKAFTNEFIP"
     gene            complement(1803..2579)
                     /locus_tag="PKI33_07350"
     CDS             complement(1803..2579)
                     /locus_tag="PKI33_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36859.1"
                     /translation="MKPKTLHTLSRWLGIASVLLAIPLWALIVRISGLPKFILPSPAD
                     VWMRFIKALSDGSLLYHTWVTLAEILLGLIVGVCFATIVGYLLAKSRMLETVLSPYLV
                     ASQAIPVVAIAPLLVIWLGDGILSKVIICALIVFFPVLVNTIVGVRAVSASLYDLMNS
                     LRATRWQILWKLELPASLPILFGGLRIGATLSVIGAIVGELVDAQEGLGFLLQLGDFQ
                     YDTSMVFVAVFMLVFLALCLYGVVTLLERRFLKWQQRSNV"
     regulatory      complement(2751..2911)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2956..>3181)
                     /locus_tag="PKI33_07355"
     CDS             complement(2956..>3181)
                     /locus_tag="PKI33_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNM36860.1"
                     /translation="LQEKIHSGVTPMTMKITEDCIACGACLPDCPTESITEGDGITYV
                     IDQATCVECEGHFDSPKCASVCPVECIVKA"
BASE COUNT          860 a          750 c          765 g          806 t
ORIGIN      
        1 atcttaattt ctttgccaat ttcccgaagt gacttgtata tcgtatatcg tctgtgcgtg
       61 gacgtggaag gtcgacttcc acgtccattt ttattttgcc ggggcgtttc gttaacacca
      121 tcacacggtc tgcaaggaaa agagactcgt ttatggaatg agtcaccatg atgaccgtct
      181 tctgttgaga ttgccaaatg ttggagagtt ctgtccacat gcgttcgcgg gtcagagcat
      241 ccagcgaggc gaaaggttca tcgagcagaa gcaggtctgg gtcatgaatg agcgcgcgcg
      301 caattgccac gcgttgtgcc attccaccag aaaggtcacg cggataggca tcttcgaatc
      361 cttttaatcc caccaacttg atcatctctc gtgctttatt ctgtgacttg atatcgccta
      421 ttccttctaa ctcaagaggc agcatgatgt tttggatcac agtgcgccaa ggcataagat
      481 tggcttgctg aaaaaccatg cctatctttt gctggtggtg atacataaaa tttacgttgc
      541 cagcggttgg ctcgagaatg cctgctaaca cgcgtaagaa ggtcgttttt cccgagccgg
      601 aaggacccag aaaacaaaca aattcacgcg gacgaacttc gaacgagatg ttatcaagtg
      661 cgtgaagccc gccattgtta tcggggtaag tgacagaaag gttgcgaaca ctgaggatgg
      721 gttttgtggt catggctaat tcgcggttca cacatcacta catgtgtgaa ccgcgactcg
      781 taaaatcctt atgggatgaa ttcgtttgta aatgctttgc tgagatcaat aggctctgga
      841 atcaacgcca tctttacaag gacgttgttc atgttttccc acgcctgtgg gttggaatac
      901 cctatccgat ctgctttcca aaattcaatc gatgtgttta agacttgcat ttgcacagcc
      961 ttatcttggt cttttaggtt ttctacgaac ttcaagctga tgtcataggc ttcttcaggg
     1021 ttattgatcg tagcttcaat accgcgtgcc agtgcttttg ccatgccgcg cacaagttca
     1081 gggtcgttcg cgatggtatc ttcattggtc acaatgccat ttgcgatcaa gtcaacataa
     1141 tctgccacac gcatttcttt taagtcaaaa ccttgagatg ctaatacgat cggttcgttc
     1201 gctgcgtagc caacgacaat gtcactattg cccgaagcat aagcttccac ttggttgaaa
     1261 ccaatggcat ccatacttac ttcagatggg tctactccgc cttcgaataa caatgcttcc
     1321 aaaccaatgt agtttgcgcc gaacaaacct ggtaagccaa ttttctttcc gcgcaggtct
     1381 gcgggttcat tgatgttcaa ctctggtgca gagatgattg agatgggaaa ttgctggtac
     1441 caagcaaaag catacacaac cggtaaccct tgtgaacgtg ccaataaaac ttgttcgccc
     1501 gatgccactg cgaatggaag ttcgcctgca cccgtcaatg caacgccatc ggtctcgaaa
     1561 gaataatcga actctatttc aattccttct tcagcaaaat accctttatc aattgctaca
     1621 tagaatggtg catattggat gttgggaata tagcccatcg gcaatttaat atgacgaagt
     1681 tcccccgccg cattcgtgga ctgtgagctg gtacagccca ttagagcgac tgtcagcgtt
     1741 agcatgagta aaactacttt cttcattaca atgtctccta agatgttttc aagttccaac
     1801 gtttacacgt tagaacgttg ttgccatttc aaaaatcttc tttccaagag cgttacaacg
     1861 ccatataaac atagcgcgag aaagaccaac ataaagactg ctacaaagac catggacgta
     1921 tcatattgaa aatcgccgag ctgtaacagg aagcccaaac cttcctgtgc gtcgactaac
     1981 tcaccgacaa ttgcaccaat caccgaaagt gttgcgccga tgcgaagtcc gccaaagagg
     2041 atgggaagcg aggctggcaa ttcgagcttc cataagattt gccaacgggt agcacggagg
     2101 gagttcatca agtcatatag ggaagcggat acggctcgta ccccgacaat cgtattgacc
     2161 aacactggaa agaatacgat tagggcacag atgataactt ttgagagaat gccatctccg
     2221 agccagatga ctaataaggg agcaatggct acaacgggaa ttgcctgact tgccaccaaa
     2281 tacggcgaaa ggacagtttc aagcatccgc gatttagcga ggagataccc aacgatcgtg
     2341 gcaaaacaaa cgccaacgat caagcctagc aaaatttctg ccagtgtaac ccatgtgtga
     2401 taaagcaaac tgccgtcact cagtgcttta atgaaacgca tccatacatc agcgggggag
     2461 ggcaggatga atttgggaag accgctgatg cgaacgataa gcgcccagag cggaatcgca
     2521 agcagaacgg aggcaatccc cagccaacga gataaggtgt gcagagtttt tggtttcatg
     2581 atcatcagtc acaggtggca aataaaaacg cctcagagta cataaaactc cggggcgtga
     2641 aaacaggcaa caatccaggc gtgaggcgcg tgaattccat aaataaaaaa cccgaaaaca
     2701 aagagcgttt tcggggtgag aatccacaca gatcccacaa ggggttgtgc ttataccttc
     2761 ttatatccag actatactgt cggctccgga gttgcgccgg atcatgcccg ttttcccaat
     2821 gaggaaagcg atgtacctaa acaggctcgt gggctgtacc accgatcggg aattcacgcg
     2881 ggttgcgtgt caccctgccc cgaaggttga atattcagtt gaggtgatta taccctcaac
     2941 aagattagga ttcaattagg ctttgacgat gcattccacc gggcaaacgg aggcgcactt
     3001 gggtgagtcg aagtgacctt cgcattcgac acaagtggct tggtcgatga cgtaggtgat
     3061 gccatcgcct tcggtgatgg attcggtggg gcaatcgggc aggcatgcgc cgcaggcgat
     3121 gcaatcttct gtgatcttca tggtcatggg agttactcct gaatggattt tttcttgcag
     3181 t
//
LOCUS       DAOREF010000301         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_19951_length_3175_cov_4.662179, whole genome shotgun sequence.
ACCESSION   DAOREF010000301 DAOREF010000000
VERSION     DAOREF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19951_length_3175_cov_4.662179"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKI33_07360"
     CDS             <1..215
                     /locus_tag="PKI33_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36861.1"
                     /translation="GIGLYGVHQFQGPVKRGADVFKQFLFLTIVVLTGFLSIYISSWL
                     LGILVLVVSILFVIISTGIVLGRHHI"
     gene            269..2245
                     /locus_tag="PKI33_07365"
     CDS             269..2245
                     /locus_tag="PKI33_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNM36862.1"
                     /translation="MNILVTLCLGGLGVLFLVLLTGIRFIPNNRIGIVEKRFGGKSLK
                     SGLIAINGETGFQPQVLRGGLHYRMPIQYAIHIAPLVTITQGKIGYVFARDGAPLEPA
                     QVLASNVTAHDFQDVTTFLKNGGQKGPQRLILREGTYAFNLMQFVVITEERVYSLPLS
                     RDDTAVIVRMAEVIKERDGFRPVIIKDGDDKIGIVTVHDGPSLPQGEIIAPVVGDNPE
                     KADEYHNKFQDADRFLKANGMRGRQLQVLVEGTYYINRLFATVEMIHKTIIDVGNVGV
                     VVSYTGEQGVDLSGSEYRHGELVTRGQRGVWSEALLPGKYPFNTYAGKVIPVPTTNFI
                     LKWIKNEVGSHNFDENLTEVSLITKDAFEPSLPLSVVVHIDYKKAPLVIQRFGDIKRL
                     VEQTLDPMVAAYFKNVAQTRTLIQLLQERSAIQQQSSLEMKEKFEHYNLELEEVLIGT
                     PSSPAGDKQIETILTQLRQRQIAEEQVETYSQQEKAAVKERELREAESRAAMQTKMTE
                     AELSISIQSNQGKAEYERSIQQAQQIRALAEAEAEKVARIGIAQALATEEQVRAYGGP
                     QFQVTQQVLNRFAEAIQQSGVDVVPRVVVGGSNGENGGNQSANIMEGLLTMLLSERFG
                     VSGEASTTQRSPDVEAMREKILKDMQKKDDTKKA"
     gene            complement(2269..2790)
                     /locus_tag="PKI33_07370"
     CDS             complement(2269..2790)
                     /locus_tag="PKI33_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36863.1"
                     /translation="MTIILETPRLILRHQVLSDLDDLWALYQDPEITKYIPDAPRSRE
                     EAQKELEWHMHGHPRRPELGLWATIHKETGKFIGRCGLLPWEIDGQNEVEVAYTIAEA
                     YQGQGLGSEAALAILNYGFSKLKLSRLICLIDPENIPSQKVAEKMGMRFEKESRDEYG
                     MFWIYSIKKEAGN"
     gene            complement(2792..>3175)
                     /locus_tag="PKI33_07375"
     CDS             complement(2792..>3175)
                     /locus_tag="PKI33_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM36864.1"
                     /translation="EDLGGYIARYEQGHFRAWGIEHRADKKLIGITNFGFLSKEDRRA
                     EMGYTIARKYWGQGFATEAAQALIRYGFEKLDLARIEAVVLPENKASSKVMLKVGMQY
                     EGLLRSYQVWKGKPSDLEIYAIVKA"
BASE COUNT          790 a          811 c          793 g          781 t
ORIGIN      
        1 tgggaatcgg gctttatggg gtgcatcagt tccaaggtcc agtaaaacga ggcgcggatg
       61 tgttcaaaca gttcctattc ctgaccattg tcgtattaac cgggttcctt tcaatttata
      121 tcagttcgtg gctgttgggc atccttgtgc tggttgtctc catcctgttt gtgattattt
      181 ccacaggtat cgtactcgga cgccaccata tctaaaattg gaaacctgaa tacgcctcac
      241 agcgtattta gatggtgaag gagaaaaaat gaatattctt gtaacgttat gtttaggtgg
      301 gcttggcgtt ctttttttgg tcttactgac cggtatccgc tttattccaa acaaccgtat
      361 cggtatcgtg gaaaaacgct tcggtggaaa gtcgttgaaa tctggcttga tcgccattaa
      421 tggcgagacg ggtttccagc cacaggtgtt gcgtggtggg cttcattacc gcatgcccat
      481 tcagtatgcg atccacattg cgcccttggt gaccattacc caaggcaaaa tcgggtacgt
      541 tttcgcgcgc gacggtgctc cgcttgaacc ggcacaggtc ttggcttcca atgtaaccgc
      601 gcacgatttt caggatgtga ccacattctt gaaaaatggc gggcaaaaag gtccgcaacg
      661 cttgatcttg cgtgaaggta catatgcatt caacttgatg cagttcgtgg tcattactga
      721 agagcgcgtg tactctctgc cgttgagccg agacgatacg gctgtcatcg tgcgcatggc
      781 agaagtgatc aaagaacgtg atgggttccg cccggtcatt atcaaggacg gtgacgacaa
      841 gatcggtatt gtgaccgtgc atgacggtcc gtctttaccg cagggtgaga tcattgcccc
      901 agtggtaggt gataacccag agaaagccga tgaataccac aacaagttcc aagatgctga
      961 ccgcttcttg aaagcaaatg gtatgcgtgg tcgccaactc caagtgttgg tggaaggtac
     1021 gtattacatc aaccgtttgt tcgccactgt tgagatgatc cacaagacca tcattgacgt
     1081 agggaatgta ggtgtggtgg tttcttacac cggtgaacaa ggcgttgacc tttccggttc
     1141 agaataccga cacggtgaac tcgtcacccg tggacaacgt ggcgtgtgga gtgaagcgct
     1201 tctaccgggt aagtatccgt tcaataccta tgcaggcaag gtcatccctg tgccaaccac
     1261 taacttcatt ttgaagtgga tcaagaacga agtcggttcg cacaacttcg atgagaacct
     1321 gaccgaagtc tcgctcatca ccaaggacgc gttcgagcct tccctgccgc tttcagtcgt
     1381 ggttcacatc gactataaga aagctccgct ggtcattcaa cgcttcggcg atatcaaacg
     1441 cctcgttgaa caaaccctcg accccatggt ggccgcatac ttcaagaacg ttgcccagac
     1501 gcgtaccctc attcaactct tgcaggaacg tagtgccatt cagcaacaat ccagcctgga
     1561 gatgaaggaa aaatttgagc actacaacct tgagttggaa gaagtgctaa tcggtacacc
     1621 ttcctcaccg gcaggtgaca aacagattga aaccatcctc actcagttac gtcagcgcca
     1681 gatcgcggaa gagcaggtag aaacctactc gcaacaagaa aaagccgcgg tgaaggaacg
     1741 cgaactacgt gaagccgaat cccgtgctgc gatgcagacc aagatgacgg aagccgagtt
     1801 gagcattagc atccagtcga accagggtaa agccgagtac gaacgctcca tccagcaggc
     1861 gcagcagatc cgtgccttgg ctgaagccga agccgaaaag gtcgcccgta tcggtattgc
     1921 gcaagcgctt gccaccgaag aacaggtgcg cgcgtatggt ggtccgcagt tccaggtgac
     1981 gcaacaagtg ctcaatcgct ttgctgaagc cattcaacaa tctggtgtgg atgtggttcc
     2041 gcgtgtggtg gtcggtggca gcaatggcga gaatggcgga aaccaatctg ccaacatcat
     2101 ggaaggcttg ctgaccatgt tgctctccga acgctttggc gtctctggtg aagccagcac
     2161 aacccaacgc tcaccggatg tggaagccat gcgcgagaag attttgaaag acatgcaaaa
     2221 gaaagacgac acgaaaaaag cctaagtcat aaaaaaagaa gctggcaatt agttgccagc
     2281 ttcttttttt attgaatata tccaaaacat tccgtattca tcccggcttt ctttttcgaa
     2341 ccgcatcccc attttttcag caactttttg cgaagggata ttctccgggt cgatcaagca
     2401 aataaggcgg gataatttca atttactgaa cccgtagttc aggatggcta acgccgcctc
     2461 actccccaag ccttgacctt gatacgcctc agcgatcgta tatgccactt cgacctcgtt
     2521 ctgcccgtcg atctcccaag gtaaaagtcc gcaacgaccg ataaacttgc ccgtttcttt
     2581 gtgaatggtc gcccacaaac ccaactcggg gcggcgcgga tgtccgtgca tgtgccattc
     2641 gagttccttc tgcgcttctt cgcgcgaacg cggcgcatca ggaatgtatt ttgtgatttc
     2701 agggtcttga tacaacgccc aaaggtcatc caagtctgaa agaacttgat gccgtaaaat
     2761 gagccgagga gtttcaagaa tgatagtcat cttatgcctt cacaatcgca tagatttcca
     2821 aatcgctggg tttgcctttc catacttgat aactgcgcag tagaccttca tactgcatcc
     2881 caactttgag catgactttg gaagacgctt tattttctgg aaggactacc gcctcgatcc
     2941 gcgctaagtc cagtttctcg aacccatacc ttatcaacgc ttgagccgct tcagtagcga
     3001 agccctgccc ccaatatttg cgcgcaatgg tgtaccccat ctcggcgcgg cggtcttctt
     3061 ttgagagaaa accgaagttg gtgatgccga tcaacttttt atcggcgcgg tgttcgatgc
     3121 cccaggcgcg gaaatgtccc tgttcgtagc gggcaatata accacccaag tcttc
//
LOCUS       DAOREF010000302         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20037_length_3168_cov_4.010601, whole genome shotgun sequence.
ACCESSION   DAOREF010000302 DAOREF010000000
VERSION     DAOREF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_3168_cov_4.010601"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKI33_07380"
     CDS             <1..555
                     /locus_tag="PKI33_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM36865.1"
                     /translation="VVAWYVGIRYPERLEKLAILNVPHPDVMTHFLLHDSEQRKKSWY
                     VYFFQLPFITEWDLSRQNYRNMARMLVGSGLKSTFSRRDIEEYKKAWSQPGALSAMIN
                     WYRSIVRESLKDLFRKRQPPRRIHVQTLMMWGMKDVALSHKMVQPSIDLCDDGKLIFF
                     EHATHWVQHDEANEVNKNLIEFLR"
     gene            560..1417
                     /locus_tag="PKI33_07385"
     CDS             560..1417
                     /locus_tag="PKI33_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNM36866.1"
                     /translation="MPILALVLLFLSASMHALWNFLLKSAEEKYVAMGWQTILSGSLA
                     LLVMVFYSGLPPKEMWLFAVISMLLEAIYFILLCIAYSDHDFSLVYPIARGAAPALLV
                     LWTAIFLHEQLTTGGYIGLAMITLGMMVIGGTTLLQSKGEKVHLKGILTALSVALVIS
                     LYTFIDGKAVKSGPALPYGLSMFVMVPFVVTPYIANQYGWDSFGRVWQKNRNYLLIGG
                     VLGLIAYMLALFAYTFAPLSYSGAIREVSAVIGAFLGWRFLKEQMGGVRVLGSAIVFI
                     GVMVIAIFG"
     gene            1858..3093
                     /locus_tag="PKI33_07390"
     CDS             1858..3093
                     /locus_tag="PKI33_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNM36867.1"
                     /translation="MQTPWEYRFAHRTQRMGSSVIRELLKFTEQPDIISFAGGLPAPE
                     VFPVNEFQEACNRVLTEHGAQALQYSTTEGYRPLREMISRHTDRYKVSVTPENILITS
                     GSQQALDFLGRLFINRGDYIVVESPTYLGALQAWNAYGAQYISVPADENGMIVEELEK
                     ALRVGPKFIYVLPNFQNPSGSTLSLERRKRLVALADQYGVPIIEDDPYGQLRYDGEHI
                     PSVVYLDAQYRNDGDHEYTGNVIYLSTFSKLLAPGLRLAWVVAPPKVIQKLVMTKQAA
                     DLHTSSFNQQVAYEVGKGGFLDEHVKVIRATYKERRDVMLEMMDEMFPAEMRWTKPQG
                     GMFLWGMMPDGVDAADVLRIAIERKVAFVPGAAFHPNGGGDNTMRINFSYSSPEMIRE
                     GITRLGSTLKEVLKKNGHH"
BASE COUNT          810 a          702 c          806 g          850 t
ORIGIN      
        1 gtggtggctt ggtatgttgg cattcgctat ccagaacgct tggaaaagct cgccatcctc
       61 aatgtgccac accctgatgt aatgacacat ttcttgttgc atgactctga gcagaggaaa
      121 aagtcttggt atgtgtattt ctttcaactg cctttcatca ccgagtggga tttaagccgt
      181 cagaattatc gcaacatggc gcgcatgttg gttgggtcag gtctcaagag cacatttagc
      241 cgccgcgata tcgaagaata caaaaaagca tggtcacagc ccggtgcctt atctgccatg
      301 atcaactggt atcgttcgat tgtgagagaa agcctgaaag acttattccg caaaaggcag
      361 cctccacgcc gcatccacgt gcagactttg atgatgtggg gcatgaaaga cgtagctctc
      421 agccataaaa tggtgcaacc gtccattgac ctgtgtgatg acggcaagtt gatttttttc
      481 gaacatgcca cacattgggt tcaacacgat gaagccaatg aagtgaataa aaatctgatc
      541 gagttcctga ggtaacggaa tgcccattct tgcgcttgtt cttctgtttc tgtctgcatc
      601 catgcatgcg ctgtggaatt ttctactcaa aagcgcagaa gaaaaatatg ttgccatggg
      661 gtggcaaacc atcttaagcg ggtcactggc tttgcttgtg atggtcttct attcaggctt
      721 gccgcccaaa gagatgtggt tatttgccgt tatcagcatg cttttggaag ccatttactt
      781 cattttgctt tgcatcgcat acagtgacca tgatttttcg ctggtctacc ccattgcccg
      841 cggagccgca cctgccctac tagtattgtg gacagctatc ttcttacatg aacaattgac
      901 caccggcggc tatattggtc tggcaatgat tacgcttggc atgatggtga tcggcgggac
      961 gactctgctc caaagcaaag gtgagaaggt gcacctcaaa ggcattctta ccgcgttgag
     1021 cgtggcgttg gtcatttcgc tctatacttt cattgatggc aaagccgtca aaagcggacc
     1081 cgcactacca tatggtttgt cgatgtttgt gatggtgccg ttcgtagtca caccgtatat
     1141 tgccaaccaa tatggctggg attcatttgg cagggtgtgg caaaaaaatc gcaattatct
     1201 gctgattggc ggcgtgttgg gattaatcgc ctatatgttg gctttatttg cctacacctt
     1261 tgcaccgctc agttattcag gtgcgattcg cgaggtgagt gcagtgattg gggcgttttt
     1321 gggctggcgc ttcttgaaag agcaaatggg cggggtgcga gtgcttgggt ctgcgattgt
     1381 gtttataggg gtgatggtga ttgcaatctt tggctaaaca gagagaaatg aaaaaagccg
     1441 cctacattga cggctttttg tttggaaatt aaagtgcaac gtccagggtg aggggggcac
     1501 cctggacgtt gcaagaccta ttttaccaaa atggcaaaaa cgcgcaaggg ttgaaaacta
     1561 ctaaatttct gagggtttga taagagaagt tgtgcacagt gtagatagat tccaatggac
     1621 agaaacaggc aaaaacaggt gaagaggcgg agcatcctgc aatttgtcac gtcttttcaa
     1681 gctatcgaca gttacaaaaa acagttgcgt cctctgccaa aaataggtaa gataaaggtg
     1741 ccatgaatgg atttctggct atattaagtt tcggcattcg attgccatga gtcagcaagc
     1801 ctgatgggta cccgcgtacc tgtcaggctt tttttatctc tacactaagg agtaagaatg
     1861 cagacaccct gggagtatcg cttcgcccac cgtacacaga gaatgggaag ctcggttatt
     1921 cgtgaattgt tgaagttcac agagcaaccc gatatcattt cgtttgccgg cggtttgccc
     1981 gcccctgagg ttttcccggt gaatgaattt caagaagcct gcaatcgtgt gttgaccgaa
     2041 cacggcgcac aagcattgca atacagcacc acagaggggt atcgcccatt gcgcgagatg
     2101 atctctcgcc atacggaccg ttataaagtt tcagtcacac ctgaaaatat tttgatcact
     2161 tctggttcac aacaagcgtt ggattttctt gggaggttgt tcatcaatcg gggggattac
     2221 attgtggtgg agtcgcctac gtatctcggc gcgttacaag cttggaatgc ctatggcgca
     2281 caatacattt cggtgcctgc ggatgagaac ggcatgatcg tagaggaatt ggaaaaagcg
     2341 cttcgggtcg gaccgaaatt catttacgtg cttccaaact ttcagaaccc ttctggctct
     2401 actctcagcc tcgaacgcag aaaacgtttg gttgcccttg ccgatcaata tggtgtgccc
     2461 atcattgaag atgatcccta tggtcaacta cgctacgatg gcgagcatat tccttcggtt
     2521 gtttatctgg atgcgcaata tcgtaatgac ggcgaccatg aatacacagg caacgtcatt
     2581 tacttgagta cgttctcaaa gttacttgcc cccggtttga ggcttgcctg ggtagtggca
     2641 ccacccaagg tcatccaaaa attggtaatg accaaacaag ccgcagacct acatacgtcc
     2701 agttttaatc aacaagtggc atatgaagta ggcaaaggcg gattcttgga tgaacatgta
     2761 aaggtgattc gcgccacgta caaagagcgc cgcgatgtga tgcttgaaat gatggatgaa
     2821 atgttcccag ccgaaatgcg ctggacaaag cctcaaggcg gcatgttctt atggggcatg
     2881 atgcccgatg gtgtggacgc tgcagacgta ttgcgaattg ccattgaacg caaagtggca
     2941 tttgtgccgg gcgctgcctt tcaccctaac ggcggtggag acaacaccat gcgaatcaac
     3001 ttctcttatt cgtcaccaga gatgatccgc gaagggatta caaggctggg ctctacgttg
     3061 aaagaagtcc ttaagaaaaa cggacatcac taagttaaat aaaaaaatcc cgattgttca
     3121 tcacatcggg atttttttat ttagtactca atcacacgtt tgagtgat
//
LOCUS       DAOREF010000303         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20161_length_3154_cov_4.598903, whole genome shotgun sequence.
ACCESSION   DAOREF010000303 DAOREF010000000
VERSION     DAOREF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20161_length_3154_cov_4.598903"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="PKI33_07395"
     CDS             <1..2055
                     /locus_tag="PKI33_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36868.1"
                     /translation="VFLQLNSDSIRIINVSDMLRNDLSAELTQNLQANIQELNSLLMR
                     GSVIVSGTSVELSKPVKDILRAFGLLNAVAIPVKGQEALKAIVLVGSQGEIPTGSSVQ
                     PYENLADLMSVALQRASAFVQTERHLQEVETLASMNELIASTSDIQDFFHALHGKIQQ
                     IIGDYSLIVAIYDEPTNSISIPFSYEAGKIQAIPTFPLGEGLTSILIRTRQPLLLVKD
                     TERRAAELGAKIVGKPAKSWMGAPLIVQNTVTGALIIQDVEHEGSFNEDDLKFFTTIA
                     SQVAGVIKNVQLLDESKQKALQVETAAEIARDISGSLNLDELLSKAVNLIRDRFNFYH
                     AGIFLLDIPGEFAIIREATGEAGAQMKRQGYKIGVGSKSIVGYVTGKGETLVVSDTTK
                     DITYYPNPLLPETRAEAAFPLRVGERILGALDVQSIQPYGFHEDNLRSLQILADQLAI
                     AVANTELFAETQEHLSQHRLLHHITSSAASGTTLEEALESAVSGLQVTLGGDRVIILL
                     VDREKRILEVKASIGYSEDISSMTVEVGKGVTGWVAAHRRPLRIRDVREDSRYIETSP
                     NTRSEMAIPLIYRNEILGVINVESELVDAYSENDEEMLGTLGGSLAAVIANARLVEQI
                     RAQSERERLINEITARIRRSTDIQSILHTTASEISRVTGARYTKIQVEPKSADTPSDE
                     RQ"
     gene            2055..>3154
                     /locus_tag="PKI33_07400"
     CDS             2055..>3154
                     /locus_tag="PKI33_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM36869.1"
                     /translation="MQSQTTSETSQQKATERVYRTWREGFIISLLVGVLIFGAVALLP
                     ALNASESIVIDGIFISTYIIVALATIFKFPYNIRVTAFLLSVYVLGIGELISLGILGD
                     GLFYFIALVIFATMLVSPRAGSVSIAISIITFIVIGWLMISGRFDPLFPVVAPAKLAD
                     WFSASAALIMFGAVIIIGFGQLEKEFAGAQAKITSTLDELREERNNLDNRVQERTNQL
                     RRINEVGRAVAAILDINEILPLAAKYIQNEFDFYYTAVYLLDITGQWADLKEAIGETG
                     KVMKERKHRVNTTGKSLVAQVIRSKTGQIMQDTNQIRQENPMFPYTRSLIVVPLVVGD
                     TILGALEMHSSKDNEFLPQDLDAYQNMANGIS"
BASE COUNT          873 a          712 c          735 g          834 t
ORIGIN      
        1 gtcttccttc aactaaatag tgacagtatc aggattatca acgtttcaga catgctccga
       61 aacgaccttt ctgctgagtt gacccaaaat ttacaagcca acattcagga actaaattca
      121 ttgttgatgc gtggttctgt catcgtatct ggcacaagcg tggaattgtc caagccggtt
      181 aaagacattc ttcgcgcctt tgggcttctc aatgcggttg ccatcccagt aaagggacag
      241 gaagccttaa aagcaatcgt actggttggc tcacagggcg aaattcccac cggttcttcg
      301 gtgcagccat acgaaaacct cgcagactta atgtctgttg cccttcaacg tgctagtgcc
      361 tttgtacaaa cagaaaggca cttacaagag gttgaaacac tagcgtcaat gaatgaattg
      421 attgcctcta catctgatat tcaggatttc ttccacgctt tgcatggaaa aattcagcag
      481 attattggcg attacagttt gatcgttgcg atctacgatg aaccgacaaa ctctatcagc
      541 attccattta gttatgaagc tggcaaaatc caggcaattc caacttttcc acttggtgaa
      601 gggttaacat caatattaat tcgtacgcgc caacccctat tactggttaa ggatacagaa
      661 cgaagagccg cagaacttgg agcaaagatc gttgggaagc cagccaaatc ttggatgggc
      721 gcgccattga tcgtgcagaa cacggtcacg ggggcattga tcatccaaga tgttgaacat
      781 gaaggctcgt ttaatgaaga tgacctgaag ttcttcacta ccattgcaag tcaggtagct
      841 ggagtcatca agaacgttca gcttctcgat gagagcaagc aaaaagcgtt gcaagtagaa
      901 actgcagctg aaatcgctcg cgacattagt ggctcattga accttgacga acttttgagc
      961 aaggctgtca acttaatccg cgatcggttt aatttctatc atgccggaat tttcttgcta
     1021 gatattccgg gtgaatttgc catcattcgc gaggcaactg gcgaagcggg tgctcaaatg
     1081 aagcgccaag gatataagat cggcgtcggc tcaaaatcga ttgtggggta cgtcactgga
     1141 aaaggcgaaa cgctggtagt cagtgatact acaaaagata tcacctacta ccccaaccca
     1201 cttcttccag aaacccgcgc cgaagccgct ttccccttgc gagttggcga acgtattttg
     1261 ggcgcattag atgttcaaag cattcagccc tatggttttc atgaagataa tctgcgaagt
     1321 ttacagattc ttgcagatca acttgcaatc gcggttgcaa acacagaatt gtttgcagaa
     1381 acccaagaac atctctcaca gcatcgtcta ttgcatcaca tcacatcctc tgccgcttca
     1441 ggtactacac tagaagaagc actggaaagc gctgtcagtg gacttcaagt aacactgggc
     1501 ggtgatcggg tgattatttt attggtcgat cgcgaaaaac gtatattgga agtgaaagct
     1561 tcgattgggt attcggaaga tatttccagc atgacggttg aggttggcaa aggtgtcacc
     1621 ggctgggtgg cggctcacag acgccccttg cggattcgcg atgtacgtga agactcgcgc
     1681 tatattgaga ccagtcccaa tactcgctct gaaatggcaa tccctttaat ttaccgcaat
     1741 gaaattctgg gtgtgatcaa cgtggaaagt gaattagttg atgcatattc tgagaacgat
     1801 gaggaaatgc ttggaacgct gggcggcagt ttggcagcag tgatcgccaa tgcccggttg
     1861 gtggagcaaa ttcgggcgca gtctgagcgc gaacgtctta tcaatgagat caccgcccgc
     1921 atccgcagat ccacagatat tcaatccatt ctacacacaa ctgccagtga gatatcaagg
     1981 gttacgggtg cgcgttatac aaaaattcag gtggagccga aaagcgccga cacacctagt
     2041 gatgagaggc aataatgcaa tctcaaacca cttcagaaac ctctcaacaa aaagcaactg
     2101 aaagagttta tagaacctgg cgcgaaggtt ttattatttc tcttttggtc ggtgtactga
     2161 tatttggcgc tgtagcactc ttacctgcgc tcaatgcttc tgaaagcatt gttatcgatg
     2221 gcatctttat atcaacttat atcatcgtgg ctcttgctac gattttcaag tttccttaca
     2281 acatccgcgt aaccgcattc ttactgagcg tgtatgtgtt aggcattggg gaactcatca
     2341 gcctcgggat cttaggagac ggtcttttct atttcattgc gctggtcatt tttgccacaa
     2401 tgctggtatc gcctagagcc ggcagtgtct cgattgcaat tagtattatt acttttatcg
     2461 tgatcggttg gctcatgatc agcgggcgtt ttgacccgct cttccctgtt gtggctcctg
     2521 ccaaacttgc tgactggttc agcgccagcg ctgcgctgat tatgttcgga gcagttatca
     2581 tcattggttt cgggcagttg gaaaaagagt ttgcaggtgc acaagcaaaa atcacttcta
     2641 cactcgatga attacgcgaa gaacgaaata atctcgataa cagagtgcaa gaacgtacaa
     2701 atcagcttcg ccgcattaat gaagtagggc gtgcggttgc agctattctt gatatcaatg
     2761 aaatcttgcc gcttgccgca aaatatatcc aaaacgaatt tgatttctat tacacagctg
     2821 tttatctctt ggatataacc gggcaatggg ctgacctgaa agaagctatt ggcgaaaccg
     2881 gcaaggtgat gaaggaacgc aagcatcgcg tcaacactac cgggaaaagt ttggttgccc
     2941 aagtgatccg ttcgaaaacg ggacaaatca tgcaagacac caatcaaatt cgccaagaaa
     3001 acccgatgtt cccatataca cgatctctga tcgtagtgcc tttagtagtt ggcgatacga
     3061 tactaggcgc tcttgagatg cattcatcaa aagacaatga atttttgcca caagatcttg
     3121 atgcttatca aaatatggca aatggaattt caat
//
LOCUS       DAOREF010000304         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20172_length_3153_cov_3.926404, whole genome shotgun sequence.
ACCESSION   DAOREF010000304 DAOREF010000000
VERSION     DAOREF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20172_length_3153_cov_3.926404"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PKI33_07405"
     CDS             <1..1577
                     /locus_tag="PKI33_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36870.1"
                     /translation="ENVSLELKQGELLLIAGSSGCGKTTLARCINGLIPRSYRGDRSG
                     KVLLHGKEVADLQIPEMAQVVGTLLQDPERQIVASNVFNEVAFGPENLGLPRAEIVSR
                     VDQALKRLNLEYLRERETFNLSGGEKQKVALAGVLTMNPSILLLDEPLASLDPASAHE
                     ALAVFRSLADEGKTVILVEHRVEDAIGIKPDRLLYMEEGKIKYLGPITDLPKEIDHTQ
                     VKLPAPWVVQRVKALDIEVKTPTQPKVEERGEPLVVFEDVDFQYDEELPLILKGVNLK
                     IYPGDLIAVLGPNGAGKSTLVKHAIGLLKPTKGRVLVEGQDTRKMSVAQIAHALGYVF
                     QSPTHMLYAPTVREELEFGPKNLAYDPLLIPKFVDESLSTVNMNEFIEYPPLALSFGQ
                     QKRTTIAAVLAMRSKILVMDEPTAGQDFANYTHFMNTLCDSSEDAQSILSANFASTLF
                     ITHDVDLAVTYANRVLLFGDKHIVADGKPQDVLKDVNQLLKYRVRPTSLLQLNLDLFS
                     KTGRFLPAEGLAAYAP"
     gene            1665..2213
                     /locus_tag="PKI33_07410"
     CDS             1665..2213
                     /locus_tag="PKI33_07410"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNM36871.1"
                     /translation="MNDKSTWEFGTRQVVYGAIGAALYGVLSWITNIFPLPAAGNITF
                     RPAVAVLVFFGAAYGPWVGLLAGFIGNTLGDALSGWGFYWNWSLGNGLIGLVAGLVAS
                     QIKNFRAQGDIIKAVIYGVIGVAVGMLFASLTEMFVGGIDINTALVGYFVPAFIGNAV
                     VTIVLLPILMVAFAAVASRRGR"
     gene            2219..3106
                     /locus_tag="PKI33_07415"
     CDS             2219..3106
                     /locus_tag="PKI33_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNM36872.1"
                     /translation="MLVTWKYRPRNTFIQSLDPRTRLIFMLCIIFGLTIPEIWDFRIL
                     LPLFAVSLTLYLMAQIEWKDVKRAWIYIGILVFFIVGLNGLLSGRGGPSSVTNLPSPT
                     VFEVPIKIPFTDIGWDVPVTVSKMWFALNQMTRMLTMAVLALPIPYTMDPNIYGTAFR
                     QMGASDKVSFTMDLAFRFLPTFARDFAITMDAQRARGYEMEKLQGGIAGRLRRLAPLI
                     IPVVMQSTVTGEEVIDAMDLRAFGTKPRTWLKDLKYAPRDYVLLGLGVGIFLTCCILK
                     WGFGIGEFFVPDFFLNLFV"
BASE COUNT          700 a          716 c          831 g          906 t
ORIGIN      
        1 tggagaatgt ttctcttgag ctgaagcagg gcgagttgtt attaattgca ggctctagcg
       61 gctgtggcaa gaccacgctg gcgcgctgta tcaacggttt gattccgcgt agttatcgtg
      121 gcgaccgtag tggcaaggtt ttgcttcatg gcaaagaagt tgccgacttg cagattcctg
      181 aaatggcgca ggtggtgggg actctgttgc aggaccctga acgtcagatc gtagccagta
      241 acgtattcaa tgaggttgcc ttcggacccg aaaatttagg tctgccccgt gctgagattg
      301 tgagccgtgt agaccaagct ttgaagcgcc tcaatcttga atatttgcgt gagcgcgaga
      361 cctttaactt atcgggtggt gagaaacaga aggtggcact tgccggcgtg ttgacgatga
      421 atccatccat tcttttgctg gatgaacctc tcgcctcact cgacccggca tctgctcatg
      481 aagctttagc agttttccgc agtttggcag atgaaggcaa aactgtgatc ttggtggaac
      541 atcgtgtaga agatgcaatt ggtattaaac cagaccgctt gctttatatg gaagaaggca
      601 aaatcaagta cctgggtccg atcacagact tgccaaaaga aatagaccat acacaggtca
      661 aactgccggc gccgtgggtg gtgcaacgag tcaaggcgtt ggatatcgaa gtcaagacgc
      721 cgactcagcc taaggtcgaa gagcgaggcg agcctttggt cgtctttgaa gatgtggatt
      781 tccaatatga tgaggaactt ccacttatct tgaaaggtgt caacttaaag atttatcccg
      841 gcgatctgat cgcggtgctt ggaccgaatg gggcgggcaa atctactttg gtgaagcatg
      901 ccattgggtt gttgaagccc acaaaggggc gtgtactggt tgaagggcaa gacactcgca
      961 agatgagtgt ggcacagatt gcgcatgcgc ttgggtatgt tttccaaagc ccaactcata
     1021 tgttgtacgc gccgacggtg cgcgaagagt tggaatttgg acccaagaac cttgcatatg
     1081 acccattgct tattccgaaa tttgttgatg aaagtttatc caccgtcaac atgaatgaat
     1141 ttattgaata tccgccattg gcattgtcat ttggtcagca aaagcgcacg accattgcgg
     1201 cagtgttggc gatgcgctcg aaaatcctcg ttatggatga gcctacagcc gggcaggatt
     1261 ttgcaaatta cactcacttc atgaatacgt tgtgcgactc ttctgaagat gcgcaatcca
     1321 tcctctctgc aaattttgcc tctactttgt tcatcacaca tgatgttgac ttggcagtaa
     1381 catacgccaa ccgagtgctg ttgtttggtg ataaacacat tgtggcagat ggtaaaccgc
     1441 aagatgtatt gaaggatgtg aatcagttgt tgaagtatcg cgtgagacct acttcgcttc
     1501 tgcaacttaa ccttgatttg ttttcaaaga ccggaaggtt tttacccgct gaaggtttgg
     1561 cggcatatgc accataaatt cccctgcaac gtgaacccgt atgcagggtt tatttaaaca
     1621 aggtcagcgt tcgaatcatt cactttttta aaaggagaga agtcatgaac gataaaagca
     1681 cttgggaatt tggaactcgt caggtcgtgt acggtgcgat cggcgctgca ttgtacggtg
     1741 tcctttcgtg gatcaccaat atcttccctc ttcctgcagc cggtaacatt accttccgcc
     1801 ctgcagtggc tgtgttggtc ttcttcggcg cagcgtatgg tccttgggtt ggtttgttgg
     1861 ctggtttcat cggcaacacc ctcggcgacg cgttgagcgg ctggggcttc tactggaact
     1921 ggagccttgg taacggcttg atcggtttgg tcgctggttt ggtggcttcc cagatcaaga
     1981 acttccgcgc acaaggcgat atcatcaagg ctgttatcta cggcgtgatc ggtgttgcag
     2041 tgggtatgtt gtttgcctct ctcacagaaa tgttcgtggg cggcattgac atcaacacgg
     2101 ctttggttgg ttactttgtg cctgccttca tcggcaatgc ggttgtgacc atcgtgcttc
     2161 tgcccatctt gatggttgcg ttcgctgcag ttgcttcaag gcgaggtcgc taaaactcat
     2221 gttggtcacc tggaagtacc gtccgcgtaa tacgttcatt caaagcctgg atccaagaac
     2281 cagattgatc ttcatgctct gcatcatctt tgggctgaca attcctgaga tctgggattt
     2341 tcgcatcctt cttccgctct ttgccgtttc gctgaccctg tatcttatgg cacagattga
     2401 atggaaggat gtaaaacgcg cctggattta tattggcatc ttggtcttct tcattgtggg
     2461 cttgaacgga ctcctttcag gacgcggtgg tccttcctcg gtgaccaacc ttccttctcc
     2521 tactgttttt gaagttccta tcaaaatccc ttttactgat atcggctggg atgtgccggt
     2581 aacagtttct aaaatgtggt tcgctttgaa ccagatgacc cgtatgttga ccatggctgt
     2641 ccttgctctt ccgattccct atacgatgga ccccaatatt tatgggacag cttttcgcca
     2701 gatgggcgcc tcggataagg tctcgtttac catggacctt gccttccgct tcctcccgac
     2761 ctttgcgcgt gattttgcca tcaccatgga tgctcaacgg gcgcgcggat atgaaatgga
     2821 gaaattgcaa gggggcattg ctggacgtct gcggcgactt gcacctttga ttatcccggt
     2881 cgtgatgcaa tctacagtga cgggcgaaga agtgatcgat gccatggatt tgcgagcatt
     2941 tggcaccaag ccacgcacat ggcttaaaga cctgaaatat gcgccgcgtg attatgtatt
     3001 gcttggtttg ggggtgggga tttttctcac ctgttgtatt ttgaagtggg gttttggcat
     3061 aggtgaattt ttcgtgccag actttttctt gaacttgttc gtctaacctg aaaaatcaaa
     3121 accgccgctt aaagcggcgg tttttttatt tat
//
LOCUS       DAOREF010000305         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20205_length_3150_cov_5.715670, whole genome shotgun sequence.
ACCESSION   DAOREF010000305 DAOREF010000000
VERSION     DAOREF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20205_length_3150_cov_5.715670"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1554)
                     /locus_tag="PKI33_07420"
     CDS             complement(232..1554)
                     /locus_tag="PKI33_07420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM36873.1"
                     /translation="MDPQNTDLPIGSLRIGLFTDTYAPQVNGVSVSLQMISEGLKKRG
                     HQVTIFAPKFPGYKDNEPNVMRLPSLKYLNNPPIYVAVLGTPRSTWKLTRKHFDVLHA
                     HSPASVGLLAYLTASTKRLPLIYTYHTSITDYTHYIKFIGNTGIIKYAASWFSKASTD
                     LGDQIIVPSPKFYRLLLNQKVKQPISIIPNGIDLSMFKVAKNPGALRKRLGLGADVPI
                     MLTVGRMDPEKRLDFIVEAFDLIADRVPNAHLVFAGDGGARKHVEEKANSTRAKDRIH
                     FLGMVNRADLPDILHDANVFLSASTTEVHPISVIEAIASGLPFVAVQDEAFEGMLEEG
                     QNGYTVPLDVKKYADVLADLLPDRERLQRFGAHSRELSEKYSIEAQVKALEKLYMEAI
                     LQNWRGTLFKRMIPRQLNEIPQRISKEINEFPQKITKEINKILPGKKD"
     gene            complement(1578..1805)
                     /locus_tag="PKI33_07425"
     CDS             complement(1578..1805)
                     /locus_tag="PKI33_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNM36874.1"
                     /translation="MLPDLQMNDHLRLRKPHPCGSYEWTVVRLGADIGLECKGCQHRV
                     MLTRRELTKRMKTNLSQQEREKHRDSEEHNT"
     gene            2011..2964
                     /locus_tag="PKI33_07430"
     CDS             2011..2964
                     /locus_tag="PKI33_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM36875.1"
                     /translation="MNKDFTVKFWGVRGSYPTPGASTVQYGGNTACVEVNAGGRTIIL
                     DAGTGIIPLGRELAKRQNLELLILFSHLHHDHTQGFPFFVPAYMPKAKLHIYGPGGTA
                     ETVKHVLEHNQSSDTFPISLRDMASNKDIQSLRETQVIVWDEDGVRVHESSPSPSPEA
                     VVIRIHKSYAHPGGVYHYRIEYQGKSIVYATDTEGYVGTDRKLVNFTRNADVLIHDAQ
                     YSDDHYYGCLAGFPATQGYGHSTATMAGQVAASAETGQLILFHHDPAYADDWVAAQER
                     AAKNIFPDSLAAFEGMTLALTPQPLSQRARGVKEVKYVTHG"
     gene            2948..>3150
                     /locus_tag="PKI33_07435"
     CDS             2948..>3150
                     /locus_tag="PKI33_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNM36876.1"
                     /translation="MLRMVELKKDDTRNLLSDIRLFEGLAPAQLDWVAHRAHRRVFPT
                     GKNVMLIEQPGEAVYVILHGTVKV"
BASE COUNT          779 a          767 c          799 g          805 t
ORIGIN      
        1 cttgtgtgaa ggtggtcttg cctgcaccaa ggttgccttg taaacaaata acatctcccg
       61 cctcaagcaa agtgccaagg cgcataccga ttcttcttgt ttgttcgggg cttctgctga
      121 aaaactccag catgtgggca tcaaggatgg acatcgggtt ggaattatac cccccgcttt
      181 gctagttaca aattacgggt tacgcattgt ggatacgtaa cccgtaatca ttcagtcttt
      241 tttccctggc aggatcttgt taatttcctt cgtgatcttt tgcggaaatt cattgatctc
      301 tttcgaaatt ctttgaggaa tttcgtttaa ctgtctggga atcatgcgtt tgaagagcgt
      361 tccgcgccaa ttttgaagga tggcttccat atacagtttt tccaaggctt tgacctgcgc
      421 ttcgatggaa tacttttcgc tcagttcgcg agagtgtgcg ccaaaacgct gtaaccgttc
      481 acggtctggg agcaagtcgg caagtacatc agcgtatttc ttcacgtcga gcggaaccgt
      541 atagccgttt tgcccttctt caagcatacc ttcaaaggct tcatcctgca cggcaacgaa
      601 tggcaaaccg gaagcgattg cttcaataac agaaatagga tgaacttctg tcgtagacgc
      661 ggagaggaac acattggcat catgcaaaat atcgggtaag tctgcgcggt tgaccatacc
      721 aaggaagtgg atacggtctt tggcgcgagt ggaatttgct ttttcctcca catgcttgcg
      781 ggcgccacca tccccggcaa agacgaggtg agcgtttggt acacggtctg caatcaagtc
      841 aaaagcttcg acgatgaaat caaggcgttt ctctgggtcc atgcgaccaa cggtgagcat
      901 aatcggcaca tcggcaccta accccagtct ctttctcaat gcgccggggt ttttcgcgac
      961 cttaaacatg ctcaggtcaa ttccatttgg aataatactg ataggttgtt tcactttttg
     1021 attcaaaagt aaacgataga acttcggaga agggacgatg atctgatccc ccaagtctgt
     1081 agatgcctta ctgaaccatg atgccgcata tttgataatg cccgtattcc cgatgaactt
     1141 gatgtaatga gtgtaatcag taatagacgt gtgatacgtg tagatcaatg gcagtcgctt
     1201 tgtggaagct gtgagatacg ccagtagccc cacacttgcc gggctgtgag catgcagcac
     1261 gtcgaaatgc ttgcgcgtta acttccacgt agagcgcgga gttcccaata ccgccacgta
     1321 aataggcgga ttgttcaaat acttcaacga cggcaggcgc atcacattcg gttcattgtc
     1381 tttatagccg gggaattttg gcgcaaagat ggtgacttga tgtccgcgct tcttcaagcc
     1441 ttctgagatc atctgcaacg agaccgaaac accatttact tgtggagcgt aggtatcggt
     1501 aaataagcca atgcgaagtg aaccaatggg gagatctgta ttctgtgggt ccatgtatgc
     1561 tccttgtttg tatttatcta cgtattatgt tcttctgagt ctctatgttt ttcccgttcc
     1621 tgttgagaca agttcgtttt catgcgtttt gtcagttcgc ggcgggtgag catgacacga
     1681 tgctgacagc ctttgcattc caaaccgata tctgcgccga gccgcaccac ggtccattcg
     1741 taagagccgc agggatgtgg tttgcgcaaa cgtaggtgat cgttcatttg caggtctgga
     1801 agcatacaaa ggattatacc tttacgacca tagacgatag accatgaaaa accgtccatc
     1861 gtccgcactt gggaaatagt tctcacctgc ccagagaaca ctttccttga aggatacaaa
     1921 gtttggaaca taatacaagt gtcttggagg tgacaaagct tcagaaagaa gaccgtcatc
     1981 tccaagaacc tcaacaaatt ggaggcagac atgaacaaag atttcacagt caaattctgg
     2041 ggcgtgcgcg gaagttaccc gacgccgggt gcaagcaccg tccaatatgg tggcaacacg
     2101 gcttgcgtgg aggtcaacgc aggcgggcgg actatcatcc tcgatgcggg cacgggcatt
     2161 attccgcttg ggcgtgagct tgccaaaagg cagaatcttg aattgctgat tctgttcagt
     2221 catctgcatc acgaccacac ccagggattt ccgttctttg tgcctgcgta catgccgaaa
     2281 gcaaaattgc acatttatgg tcccggcggc acggctgaga cggtgaaaca tgtgctggag
     2341 cacaatcagt cttcagacac gttcccgatc tcattgcgtg acatggcgtc gaacaaagat
     2401 attcaatcgc tgcgcgagac gcaggtcatt gtttgggatg aggatggagt cagggtccat
     2461 gaatcaagcc caagtccgag tcctgaagcg gtggtcatcc gcattcataa atcgtatgcg
     2521 catcccggcg gcgtctatca ctatcgcatc gaatatcagg gcaagtccat tgtgtacgcc
     2581 actgacaccg aaggatatgt aggcacagac cgcaagttgg tgaacttcac gcgcaatgcc
     2641 gatgtgctca ttcatgatgc gcaatattca gacgatcatt attacggatg ccttgctgga
     2701 tttcctgcga cgcaaggata tgggcactcg accgcaacca tggcaggaca agttgccgcc
     2761 tctgccgaaa cgggacagtt gatcctgttc catcacgacc cagcctatgc tgacgattgg
     2821 gtggcggcgc aagaacgtgc tgcgaaaaat atttttcctg attcactagc ggcgtttgag
     2881 ggaatgacac ttgccctcac cccccagccc ctctcccaaa gggcgcgggg agtaaaggaa
     2941 gtaaaatatg ttacgcatgg ttgaactcaa gaaagacgac actcgcaatc tgctttcaga
     3001 cattcgtttg tttgaagggc ttgcccccgc ccaattagac tgggtagctc accgcgcgca
     3061 tcgccgcgta tttccgaccg gcaagaatgt gatgctgatc gaacagccag gcgaagcggt
     3121 gtatgtcatc ctgcatggga ccgtgaaggt
//
LOCUS       DAOREF010000306         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20206_length_3150_cov_3.738611, whole genome shotgun sequence.
ACCESSION   DAOREF010000306 DAOREF010000000
VERSION     DAOREF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20206_length_3150_cov_3.738611"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKI33_07440"
     CDS             <1..662
                     /locus_tag="PKI33_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM36877.1"
                     /translation="VTINGRTRNLMGYLEDFLFTRDRVRAPITALSGGERNRLLLARL
                     FAMPANLLILDEPTNDLDIETLEILEDLLLDYEGTLLLVSHDRAFLNNIVTSTLILDG
                     SGDVKEFAGGYEDWKKQLDEVPASKPNIKPAQTTQTESKSDSKPAVRKLSYNEKRELE
                     ELPQRIQKLEEEQHQLTVKMEAPEFYQQDGSIITQAVERLEQIQKELSQAYERWGELE
                     G"
     gene            complement(702..1949)
                     /locus_tag="PKI33_07445"
     CDS             complement(702..1949)
                     /locus_tag="PKI33_07445"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNM36878.1"
                     /translation="MLTELGKRARLASRKLARLNATQKNAALHTLAEALLADQIMILA
                     ANLSDVESAKAAGLAPAMIDRLMLNEARLADIAHSLRLVADLADPVGEIFEGSTQPNG
                     LHIRKQRVPLGVLGVIYEARPNVTVDVAGIAIKSGNAAILRGGSETLNTNRAMIATLQ
                     KALASSDVPADAIQFIDSPDRALVLDLLHLRDYVDMIIPRGGAGLHEFCRENSHIPVI
                     TGGIGICHLFVDDTADQDAALKIIHNAKTQRPSVCNALDTVLVHKDVAKEFLPRVVEH
                     LAPAGVTFRAEAKAMVLLKHESVHPAGEKDFDTEWLSLVLGLKVVEGLDEAIEHIATH
                     STAHSDGILTRTDEHAQRFIAEVDSAAVYVNASTRFTDGTELGLGAEVAVSTQRLHAR
                     GPMALRELTTYKWVIVGDGHVRK"
     gene            complement(2039..3136)
                     /gene="proB"
                     /locus_tag="PKI33_07450"
     CDS             complement(2039..3136)
                     /gene="proB"
                     /locus_tag="PKI33_07450"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNM36879.1"
                     /translation="MLIIIKLGTSTLTAGTKKISPPFLVDLARQIHHLQNNGHQVIIV
                     SSGAIAAGREKLGQPQLPKGLPAKQMLAAIGQLRLMAIYEQVFGLYGSTIAQVLLTRA
                     DLADRRRYLNARSTLEALLAHGAIPIINENDTVATEEIRVGDNDNLSALVANVVEADL
                     LILLTDQQGLFTADPRNNPDAKLVEVIDTPEIPQEVWDSAGGSGTKLGTGGMFTKLQA
                     ADLARRSGTTVVIAQGNQANILTRLADGEKLGTRFMPVISALESRKRYILSGRLSPGQ
                     ITVNEGAASALKKGGSLLPIGMTDIAGDFDRGDTVRVLDSNGRELARGITTYSSADLI
                     RLRGHHSGEIESLLGYDYGDEVIHRNDLVIL"
BASE COUNT          682 a          843 c          908 g          717 t
ORIGIN      
        1 ccgtcaccat caatgggcgc acgcgcaacc tgatgggcta tctcgaagat tttctcttca
       61 cgcgtgaccg tgtccgcgcg cccatcaccg cgctttcggg cggcgagcga aaccgacttc
      121 tgttggcgcg tctcttcgcc atgcccgcca atctgctcat tctcgacgag cccaccaacg
      181 acctcgacat cgaaacgctt gaaatccttg aagacctgct gctcgactac gaaggcacgc
      241 tcctgctcgt cagtcatgac cgcgcctttt tgaataacat cgtcaccagc acgttaattt
      301 tggatggctc tggcgacgtg aaagaattcg ccggcggata cgaagactgg aaaaaacaac
      361 tcgacgaagt gcccgcctca aaacctaaca tcaagcctgc tcaaacaacc caaacagaat
      421 ccaagtctga tagtaagccc gccgtccgca agttaagcta caacgaaaaa cgcgagctcg
      481 aagaactgcc tcagcgtatt caaaagctcg aggaagaaca gcaccaactc acagtcaaga
      541 tggaagcgcc tgagttttat cagcaggatg gttccatcat cacccaagcc gttgagcggt
      601 tggagcaaat ccaaaaagaa ctctcccaag cctacgagcg ctggggagag ttggaagggt
      661 aatcttgagg tgacagtcaa tttcaaagtg actgtcacct tttacttcct cacatgccca
      721 tccccaacga tgacccactt atacgtggtc aactccctta acgccatcgg accgcgcgcg
      781 tgcaatctct gcgtagacac ggctacttcc gcgcccaagc cgagttcagt tccatcggtg
      841 aagcgtgtac tggcgttgac gtaaaccgcc gccgaatcca cttcagcgat gaagcgttgg
      901 gcgtgttcgt cggtgcgagt gaggattccg tcggagtggg cggtgctatg tgtggcgatg
      961 tgctcaatgg cttcatccaa cccttcaacg actttcagcc caagcacaag cgaaagccat
     1021 tcggtatcaa aatctttttc tccagcaggg tggacggatt catgtttcag caaaaccatt
     1081 gctttggctt cggcgcgaaa ggtgactcct gcgggggcga ggtgttccac cacacgcggt
     1141 aaaaattctt ttgcaacatc tttatgaacc aaaacggtat cgagcgcatt gcacacgctg
     1201 gggcgttgag tcttggcgtt gtggataatt ttcagcgctg cgtcttggtc ggcggtatcg
     1261 tcaacaaaaa ggtgacaaat gccaatgccg ccggtaatga cggggatgtg actgttctcg
     1321 cggcagaact catgcaagcc tgcgccgccg cgcggaatga tcatatcgac ataatcgcgc
     1381 aggtggagca gatcaagcac tagcgcacgg tcggggctgt caatgaattg gatcgcgtcg
     1441 gcgggaacgt cactggaggc aagcgccttt tgcagggttg cgatcatggc gcgattggtg
     1501 ttcaaggttt cactgccgcc gcgcaggatg gcggcatttc cgcttttgat ggcaatgccc
     1561 gccacatcca cggtgacgtt gggacgggct tcatagatca cgcccaacac gcccaacggc
     1621 acacgctgtt tgcggatgtg taacccgttt ggttgcgtag acccttcgaa gatttcaccc
     1681 acggggtcgg caaggtctgc cacgaggcgc aggctgtgag cgatgtctgc gaggcgggct
     1741 tcgttcaaca tcaggcggtc gatcatggcg ggggcaagcc ctgccgcttt ggcagactcc
     1801 acgtcgctta aatttgccgc gaggatcatg atctggtcgg cgagtaaggc ctcggctagg
     1861 gtgtgaagcg cggcgttctt ttgagtcgca tttaggcggg caagtttccg tgaagcaagg
     1921 cgggcgcgtt ttccgagttc tgttagcata ataattcctt tagaggtcag accatggacg
     1981 atttaccata gaccgtagaa aaccatccat cgtctgtggt ctgtcgtcta tcgtcggttt
     2041 acaaaatcac caggtcattt ctgtgaatca cttcatcgcc gtaatcatag ccaagcaggg
     2101 attcgatctc gcccgagtga tgaccgcgca agcggattag gtcagcgctg gagtaggtgg
     2161 tgatgccgcg tgccagttcg cgaccgtttg agtcgagcac gcgcaccgtg tcaccgcgat
     2221 caaaatctcc agcgatgtcc gtcatgccga tgggcaggag gctgccgcct tttttcagcg
     2281 cagatgctgc gccttcgttg accgtgatct gacccggcga aagcctgccg gaaaggatgt
     2341 aacgtttgcg gctttcgagc gcggagatga cgggcatgaa acgtgtgccg agtttctcac
     2401 cgtctgcgag gcgggtgagg atgtttgctt ggttaccttg cgcaatgacc actgttgtac
     2461 cggagcggcg tgcaagatct gcggcttgca gtttggtgaa cataccaccc gtgccgagtt
     2521 tggttccgct gccgcctgca gagtcccaaa cctcttgcgg aatctccggc gtgtcgatga
     2581 cctccaccag tttggcgtcg gggttgttgc gtgggtcggc ggtgaagagt ccctgctggt
     2641 cagtgagcag aattagcaga tcggcttcga ccacgttggc aaccagcgcg gagagattgt
     2701 cattatcacc cacgcgaatc tcttcagtgg caaccgtgtc attctcgttg atgatgggga
     2761 ttgcaccgtg cgcgagcagt gcttcgagcg tgctgcgggc gttgagatac cggcgacggt
     2821 cggcgaggtc tgcacgggtg agcagcactt gggcaatagt gctgccatac aagccaaaga
     2881 cctgttcata aattgccatc agccgcagtt gaccgattgc cgccagcatt tgtttggcag
     2941 gcaaaccttt gggcagttgc ggctgaccta atttttcccg tcccgctgcg atggcgccgg
     3001 aggagacgat gatgacttgg tgtccattgt tttgcaggtg atggatctgc cgggctaggt
     3061 caacgagaaa aggcggggat attttttttg tgccagccgt aagggtggag gttccgagtt
     3121 tgatgatgat gagcatggaa aaattgttct
//
LOCUS       DAOREF010000307         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20252_length_3146_cov_4.265933, whole genome shotgun sequence.
ACCESSION   DAOREF010000307 DAOREF010000000
VERSION     DAOREF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20252_length_3146_cov_4.265933"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKI33_07455"
     CDS             <1..299
                     /locus_tag="PKI33_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HNM36880.1"
                     /translation="LNQHLTNNRDYLVDYVTKFMPGVRTTIPAATYLGWLDFTQSDIE
                     GSPFEFFLKNARVAVSEGKIFGKEGAGHVRINFGTSHKLLKEGLDKMKNAMEMI"
     gene            complement(621..1142)
                     /locus_tag="PKI33_07460"
     CDS             complement(621..1142)
                     /locus_tag="PKI33_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36881.1"
                     /translation="MNRLEWQEKYFQKVAESSEEGAEAVRYVREKKIRVGMRRARKSV
                     GAFWTWEHAFYLNKVHYTMESALENPRAWTLFVHEVRHLQQGIPTAMSVYGELDAWQY
                     EFRLFKKITGKTLKPELEEILSLPLNFDRANLRYAQKLMTKFGGFWYAAWILPLYPIH
                     KEILYWVTRKQFA"
     gene            complement(1150..1587)
                     /locus_tag="PKI33_07465"
     CDS             complement(1150..1587)
                     /locus_tag="PKI33_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36882.1"
                     /translation="MQKYGFILILLAVLVIGCKGEEKSYASPTLSEEWTIKMTQSGGI
                     MGLRRSIQVASDGSYLIIDERAQTSVTGTLTEQQLSELTDLIANLKFISPDNRAICAD
                     CFVYDLEIESGGKKMILQVEDINLPNSGIEALVTFLRGILESA"
     gene            1718..2866
                     /locus_tag="PKI33_07470"
     CDS             1718..2866
                     /locus_tag="PKI33_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNM36883.1"
                     /translation="MRKVAILGIGQTKVDEHWDKSLREIGGEAAFLAMQDAGMDKVDA
                     LYVGNMLSPMVSGQNQLGAFFSDWIGLWKQEAVKIEAACGSGSAAFRSALMAVASGDV
                     ESALVVGVEKMTDKAGKDVTAALATAADADYEVEQGISFVGINALVMRRYMHEFGWKH
                     EDFAPFSINAHANAMHNPYARLHEKVSVEKFEKSSMVATPINLLDASPVGDGAAAVII
                     VPADKVMRQPKVVVAASGAATDTIAVHSRKDPMFLNASYLSSQQAYKMAGITPEDIDV
                     FELHDAFSIMAALSLEATGFAERGQGVRLGLENQIGIKGKIPVCTRGGLKARGHPVGA
                     TGMYQIVEVAQQLRGECGPTQVDGATVGMAQNIGGSGATILTHILRRE"
     gene            2963..>3146
                     /locus_tag="PKI33_07475"
     CDS             2963..>3146
                     /locus_tag="PKI33_07475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNM36884.1"
                     /translation="MANEKRKLPRRNFSYYMRVLKKDTGELIGQIIDISTGGFKLESS
                     KPLPSNVQMELCIDQVN"
BASE COUNT          839 a          686 c          769 g          852 t
ORIGIN      
        1 atttgaatca acacctcact aataaccgag attatttagt ggattacgtc accaaattca
       61 tgccaggcgt tcgcacaacc attccggctg ctacttacct cggctggtta gactttaccc
      121 aaagcgacat cgaagggtca ccctttgaat tcttcctgaa gaatgccaga gtggcggtga
      181 gtgaaggaaa gattttcggc aaagaaggtg ctgggcatgt acgcatcaac tttggaacat
      241 cgcacaaact tttgaaagaa ggtttggata aaatgaaaaa cgcaatggaa atgatctaat
      301 caaatccctg cgaaatataa aacgcctatc ctgatggata ggcgttttat atttaaacaa
      361 aaagacggat gggagtttac ccatccgtcg agacgaacta tgtcggggaa gaggcgaaga
      421 atgcagcctt cctttcgtta ggggaacgtc tggtacgaag caaccaacac acttgaccag
      481 cttatggcgt atcgcccttc ccttcggttt gcatttcagg tggtgtaacg atcgcaacct
      541 cctttcttat tcctattcac atagtacaac gaaaataacg ctttacatta aaaagacttt
      601 gtaattttga aattggttgt tcaggcaaat tgttttcgtg taacccaata tagaatctcc
      661 ttgtgaatcg ggtacaaggg caatatccat gcggcatacc aaaaaccacc aaatttggtc
      721 atcaattttt gggcatatct taaattcgcc cggtcaaagt taagtggcaa agaaagtatc
      781 tcttctaatt ctggtttgag tgttttccct gtaattttct tgaataaacg aaattcatat
      841 tgccaggcat ccaactcacc atatacagac atggctgtag gaattccttg ttgcaagtgt
      901 cgcacttcat gcacaaagag agtccaagcc cgtggatttt caagcgcgga ctccatcgtg
      961 tagtgaacct tgttcaaata aaaggcatgt tcccacgtcc agaatgcgcc tacactcttc
     1021 cgcgcccgcc tcattccaac ccggatcttt ttctcgcgta catagcgtac cgcctctgca
     1081 ccctcttcag aagactctgc tactttctga aaatattttt cctgccattc caaacgattc
     1141 atagccgtat caagcagact ctaaaattcc gcgcaagaag gtgaccaatg cttcgattcc
     1201 tgaattgggc aggttgatat cttctacttg gaggatcatc ttctttccgc cgctttcgat
     1261 ctcaaggtca tagacaaagc aatcggcaca gatcgcgcga ttatcgggcg aaataaattt
     1321 tagatttgca attaagtctg ttaattcact taattgttgt tcagtcagag tacctgtcac
     1381 cgaagtttgc gcacgttcat ctatgatcaa gtaacttcca tctgaggcga cctgaatgga
     1441 acggcgtaat cccatgattc cgcctgattg agtcatcttg attgtccatt cttcagaaag
     1501 ggttggggag gcgtatgatt tttcttctcc tttacagcct atgaccagca cagcaagtag
     1561 gatcaatata aagccatatt tctgcatgag gtttctcctt aggtattatc taggatagca
     1621 taaacgggat atggggctat cctaccaaat aaccagacgc tcaaaaattc agatagttcc
     1681 tgagaggcga atacgataga atcagggctg aggtgttatg agaaaagtag ccattcttgg
     1741 gatcggtcag acaaaagtag atgaacattg ggataagtcc ctgcgcgaga tcggcggaga
     1801 agccgcattt cttgcgatgc aagatgccgg catggacaaa gtcgatgcct tgtatgtggg
     1861 caatatgctt tctcccatgg taagtggaca gaatcagctc ggtgcctttt ttagcgattg
     1921 gatcgggttg tggaaacagg aagcggttaa gattgaagcg gcttgtggct ccggttcggc
     1981 ggcattccgt tcggcgctga tggcggtcgc ttcgggtgat gttgagtccg ctttggtggt
     2041 tggtgtggaa aaaatgaccg acaaagccgg taaagatgtt accgcggcgt tggcaaccgc
     2101 agccgatgcg gattatgaag tggaacaagg gatttcattt gttggtatca atgcccttgt
     2161 gatgcgacgt tacatgcacg agttcggctg gaagcacgaa gattttgcac cgttctccat
     2221 caatgcacat gccaatgcca tgcataatcc ctatgcgcgc ttacatgaaa aggtaagtgt
     2281 tgagaaattt gagaaatcat ctatggtggc aacgcccatc aatttgttgg atgcttcccc
     2341 ggtaggggat ggggcggcgg cggtgattat cgtccctgct gataaggtga tgcgacagcc
     2401 caaagtggtt gtagccgctt ccggtgctgc cacggataca atagccgtcc attcgcgcaa
     2461 agacccaatg tttttgaacg cttcttatct ttcttcacaa caggcgtaca aaatggctgg
     2521 cattacgcct gaagatattg acgtctttga gcttcatgac gctttttcca ttatggcggc
     2581 attgtctctc gaagcgacgg gtttcgcgga gcgcggtcag ggagttcggc tcgggctgga
     2641 gaatcagatt ggaatcaaag gaaagatccc agtctgcacg cgaggcgggt tgaaggcgcg
     2701 tggtcatcct gtaggggcaa cgggtatgta tcagatcgtg gaagttgctc aacaattgcg
     2761 cggcgaatgt ggaccaaccc aggtggacgg ggcgactgtg ggtatggctc aaaatatcgg
     2821 cggttctggt gcgacgattc tgactcatat tttacggcgc gagtgattat tacaatttca
     2881 taatttttca tctggttttc aggcacctcc ttggcatttg cactaaaatt aagacgcctt
     2941 tcaaatttgc aaggaggacg agatggcgaa cgaaaaacga aaactaccgc gtagaaattt
     3001 ttcctattac atgcgcgtgc tgaaaaaaga tactggtgaa ctgattggtc agattattga
     3061 tattagcacg ggcggtttta agctggaaag cagcaaaccg ctaccgagta atgtgcaaat
     3121 ggaactttgt attgatcagg tgaatc
//
LOCUS       DAOREF010000308         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20287_length_3142_cov_4.002268, whole genome shotgun sequence.
ACCESSION   DAOREF010000308 DAOREF010000000
VERSION     DAOREF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20287_length_3142_cov_4.002268"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1851
                     /locus_tag="PKI33_07480"
     CDS             217..1851
                     /locus_tag="PKI33_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNM36885.1"
                     /translation="MLTATPYGKDFSEVESQLRLLPAPKQAQSTALGFTVETQMWQAK
                     QLSDLPELAPCTVLTTPDVVRHFSKQDANGERFVVFSEKDQRYFPRRISLRTIRYQNP
                     FDDFLADLFESRLLYKKIERQENPIQAPLPLDVPFTEGERLALQEALFLHQFCSSPAE
                     VQNVCTQLEKGSYNYSFARQQELTAFIQERRSLIRNAIKPRNDLKLAHLLKIIEEAGN
                     EKIVVFCEYHETARSLRDGLKRLKSVKVETTVDTPDLDDLLRRFAPIANEVLPEERND
                     EEELQVLIATRSMSEGFNMQDASILVNYDLPWTVLQLAQRMGRVLRPWGAPRDVIIYN
                     FVPSTMDHKRIRHARNWEHRLQERSRQHRSLAQIPVMVHRESQQQGMTQELEMEKLGR
                     EMFLTSDDGADLDLEQVMEFLHTVEDLTTSTFYNDLAIISNQNEILKLPAGIRSAMNK
                     PGRKRLFLLLKRGKNRIDTIIADVHGKPMEESYRRDDVMRIIRCLPETAKATFESFPQ
                     EDEFDLWIERTRLNWTKQNGLDSGKIQVICALALVP"
     gene            1951..2637
                     /locus_tag="PKI33_07485"
     CDS             1951..2637
                     /locus_tag="PKI33_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM36886.1"
                     /translation="MNDRIIREKMNTMKSKGTSTENKLGLFIESYYSQLLVGIRVLIW
                     KMGMASTPKEADDLSQEILSETILTAIEIENRYDKDKSMRAWLLSIAANKIRELRTKE
                     TRHGKRMGSVTETYINSSKRTTSLQKQYKDIEGITEDEMIDHLNSYNSLQSHARKRLH
                     LSFDELISFVDTNDQEILRLAFENNLKGKDLAATLRISSGAANVRLSRAISRLKQAYL
                     ASENSKRIKK"
     gene            2634..>3142
                     /locus_tag="PKI33_07490"
     CDS             2634..>3142
                     /locus_tag="PKI33_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36887.1"
                     /translation="MNTNNLSDKREKTVQRYVSALDSGNLDDIILVLAEATEDPVLDQ
                     MIFDVNEAIEQEDNLTPIANDANLVLNLLQEHFANPETGNEKTQITIGDVAARMQADR
                     KIPSTDQEIHKHLLGINIPLPEILNIQAIHELANNLQLIASDKFWRVFRDTAIMLGIG
                     RGQAHMAAAR"
BASE COUNT         1021 a          643 c          686 g          792 t
ORIGIN      
        1 ccgagcatag aattttccta tcacaccctg acacgtaaag atggcgactc gaatttaccc
       61 atccttgaac atgaactaaa gcaagtcaat caacaaactc tgatcatttt ggatgaatct
      121 caccgactac gaaatgaaga agccaatggt ggagatttac gattaagcaa tgaccgcatc
      181 caaaaagccg cgcgagaaaa aagcgcaaaa gtattaatgc ttactgcaac gccgtacggg
      241 aaggatttct ccgaggtgga aagccagttg cggttattgc cggcgcccaa acaagcacaa
      301 agcactgctc ttggttttac agttgagact cagatgtggc aggcaaaaca actttctgat
      361 ttacctgaac ttgctccatg cacagtgcta accacccctg atgtcgtccg ccattttagt
      421 aagcaggacg ccaatggaga gaggtttgtt gttttctcgg aaaaagacca aagatatttt
      481 ccgagacgga ttagcctccg aacgatacgc tatcaaaacc cttttgatga ctttctggca
      541 gatcttttcg agagcaggct gctttacaag aaaattgagc gccaggaaaa tccaattcag
      601 gcaccattac cgttagatgt tccatttacg gaaggggaac gtttagctct ccaggaagcc
      661 ctattccttc atcagttctg ttcttctcct gcagaggttc agaatgtttg tactcaattg
      721 gagaagggta gttacaacta ttcatttgca agacagcagg aacttaccgc atttattcaa
      781 gagcgccgat ctcttatacg caatgccata aaaccccgca acgatctcaa actcgctcac
      841 cttttgaaaa tcatcgaaga agcagggaat gaaaaaattg ttgtgttttg tgaataccac
      901 gaaacagccc gatctttgag agatggatta aaacggctca aatctgtaaa agttgagact
      961 actgtagata cacctgacct tgatgatcta ttacgcaggt ttgcgcccat cgccaatgaa
     1021 gtcctgcctg aagaacggaa cgatgaagaa gaattgcagg tgttaatcgc tactcgttcc
     1081 atgtccgaag gtttcaacat gcaggatgca tcgattctcg taaattacga tctgccttgg
     1141 acggtgctgc aattggcaca acgcatggga agagtgttac gcccgtgggg tgctccgcgg
     1201 gatgtcatca tctacaattt tgtgccttcc acaatggatc acaaacggat tcgccatgcc
     1261 cgcaactggg agcatcggtt acaggaacga agccggcagc atcgttctct ggctcagatc
     1321 ccggtcatgg ttcataggga gagtcaacaa caaggcatga cccaggaatt ggaaatggaa
     1381 aagttggggc gtgaaatgtt ccttacatcg gatgatggcg cagatttgga ccttgaacag
     1441 gtaatggaat ttctgcatac cgttgaagat ctgactacaa gtacttttta taatgacttg
     1501 gcaataattt ctaatcaaaa cgagatatta aagttgccag caggtatccg tagtgccatg
     1561 aacaagcctg gtcgcaaacg attattccta ttgttgaagc gtgggaaaaa taggattgat
     1621 actatcattg ccgatgttca tggcaagccg atggaggaaa gctaccgccg ggatgatgtg
     1681 atgcggataa tccgttgtct gcctgaaacc gccaaagcga cgtttgaaag ttttcctcaa
     1741 gaagatgaat tcgatttatg gattgagcgc acaaggctaa actggacaaa acaaaatggg
     1801 ctagattcag ggaaaatcca ggtcatttgc gcactagccc ttgtaccata gggaaaataa
     1861 ttgacttcaa attgttcaat aaatgcctgt aataaatcgc cctacttacg ccatatgtct
     1921 attgtaacca taaatggctg caggaggctt atgaatgatc gaataatcag ggaaaaaatg
     1981 aatacgatga agtccaaggg tacctcaaca gagaacaaat tgggtctctt tattgaatca
     2041 tattacagcc agcttttggt tggtattcgt gtgctcattt ggaaaatggg catggcaagt
     2101 actccaaagg aagctgacga tttgtcacaa gagattctga gtgaaacaat tctgacagcg
     2161 attgaaattg aaaaccgtta tgataaagat aaatctatga gggcatggtt gttaagtatt
     2221 gctgccaaca aaataagaga actaagaaca aaagaaacca gacatggtaa acgaatgggg
     2281 tctgtcacgg aaacatacat taattcatct aaaagaacaa cctccttaca aaaacaatat
     2341 aaagacattg agggcataac agaagatgag atgatcgacc acctaaattc gtacaatagc
     2401 ctccaatctc atgcaaggaa acgacttcat ttaagtttcg atgaattaat ttcatttgtt
     2461 gacacaaatg atcaagaaat actaagactg gcatttgaaa acaacttaaa aggtaaggat
     2521 ttggcagcaa ccttgagaat ttccagcgga gcagcaaatg tgcgcctaag ccgagctatt
     2581 agtcgattaa agcaggcata tcttgccagt gaaaatagca agaggattaa gaaatgaata
     2641 caaataattt atcagataag cgtgaaaaaa ctgtacaacg ctatgtgagt gctctagata
     2701 gcggaaactt agatgacatt attcttgttc ttgctgaagc tacagaagac cctgttcttg
     2761 atcagatgat atttgacgtt aatgaagcaa tagaacagga agataattta acccctattg
     2821 caaacgacgc taatttagtt ctaaaccttt tacaagagca tttcgctaat cctgaaacag
     2881 gcaatgaaaa gacacaaatc actattgggg atgtcgcagc aaggatgcaa gcagatcgaa
     2941 aaataccttc tactgatcag gaaatccata aacacctttt agggataaac atacctctac
     3001 ctgagatatt aaatattcaa gcaattcacg aattagcaaa taatctgcaa cttattgcta
     3061 gtgacaaatt ctggagagta ttccgtgata ccgcaatcat gctaggtatt ggtcgcgggc
     3121 aagcccatat ggcagccgcc cg
//
LOCUS       DAOREF010000309         3133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20380_length_3133_cov_4.372320, whole genome shotgun sequence.
ACCESSION   DAOREF010000309 DAOREF010000000
VERSION     DAOREF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20380_length_3133_cov_4.372320"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKI33_07495"
     CDS             <1..235
                     /locus_tag="PKI33_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36888.1"
                     /translation="KDRNMKKNENVAMVIQDPKSPYRYLGMQGKVVSYTTEGADEHIN
                     LLSLRYDEEPWKYREGQKRIIFKIKPTSFDEHN"
     gene            219..845
                     /locus_tag="PKI33_07500"
     CDS             219..845
                     /locus_tag="PKI33_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNM36889.1"
                     /translation="MTNIIDLHTFLRTRRSIRRFKPDPIPDSVIREILHTATLAPSAH
                     NRQPWRFVVMTDKTSIAEFAKMMAIEFRHVLEQDQLPPEEIQKRLNRSQERISGAPVV
                     IIACVEMTEMEKYPDERRKKAEYLIATQSVANAGMQLLLAAHAEGLGAVWICSPLFAQ
                     SAVQEALRLQPTWEPQAMFLVGKPVEVPAVRERKPIEEISIFIDKKGN"
     gene            847..1761
                     /gene="cofD"
                     /locus_tag="PKI33_07505"
     CDS             847..1761
                     /gene="cofD"
                     /locus_tag="PKI33_07505"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNM36890.1"
                     /translation="MKIVAFAGGVGGAKLAQGLAQTLPPKDLTIIVNTGDDFEHLGLY
                     ICPDLDTVCYTLGGLANPETGWGRAGETWNTIANIEKLGGPAWFRLGDSDIATHLERT
                     RRLKEGKSLSEITKEFCKAWGIEATVLPMTDSPVRTMVDSDEGELAFQEYFVHRQCQP
                     RVKGFRFDGVEVAEPAQGVREALASADAIIFCPSNPWVSIDPILSVVKEVNKPVFAVS
                     PIIGGKTVKGPAAKMYSELGIEPSAYAVAKHYRTILKGFVLDTTDAAMEKEIQDLGFA
                     TLVTNTLMNQLTDRARLANDVLNFIRSL"
     gene            1764..2372
                     /gene="cofC"
                     /locus_tag="PKI33_07510"
     CDS             1764..2372
                     /gene="cofC"
                     /locus_tag="PKI33_07510"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNM36891.1"
                     /translation="MSLWAIVPVKPLRRGKSRLAGALTEEERAALNQELLERTLKTLT
                     SLKELDKVLVVSRDSHALTIARNHGAKTVQEDGQPHLNTALTRASVIAKVHATQGVLV
                     LPVDLPLLTTDDVLALIDRATKPPVVVIAPDRHHKGTNALLMVPAGQIEYDFGEGSFQ
                     RHSERALQSGARLEVVELPSLGLDLDDPEDLELIRKLEAQKV"
     gene            2487..>3133
                     /locus_tag="PKI33_07515"
     CDS             2487..>3133
                     /locus_tag="PKI33_07515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNM36892.1"
                     /translation="MTKQRVALYLQDSHDLRDGLDYAKYAEEKGFEAVWQAESRLVRD
                     AIVPMAGYAAVTNKIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRVICGLGAWWDPL
                     AANVGIDRKKPLTAMKETVQVMKKLLNMERVTFDGEFIHVKGIELDVVYGRREPRNIP
                     IMIGATGDKMMELTGEIADGAVLNYCVAPEYNDNAMELLNNGLKKSGRKMEDFDRP"
BASE COUNT          875 a          724 c          784 g          750 t
ORIGIN      
        1 caaagatcgc aatatgaaaa agaacgaaaa cgtggcaatg gtcattcaag acccaaaaag
       61 cccgtatcgt tatctcggca tgcaaggcaa agtggtttct tacacaactg aaggcgcaga
      121 tgagcatatc aacttgcttt ctttgcgtta cgatgaagaa ccatggaaat accgcgaggg
      181 gcaaaagcgg attattttta agatcaagcc gacttccttt gacgaacata attgatctgc
      241 acacctttct gcggacaagg cgctcgattc gccgcttcaa accggacccg attcctgatt
      301 cagtcatccg ggaaattctc cacactgcca ctctcgctcc atccgcgcat aaccgtcagc
      361 cttggcgttt tgtggtgatg acagataaaa cgtctatcgc tgagtttgca aagatgatgg
      421 caatagagtt caggcatgtg cttgagcaag atcaacttcc gcccgaagag attcaaaaac
      481 gcctaaatcg ctcccaagaa agaatcagcg gcgcaccagt cgtcattatc gcgtgtgttg
      541 aaatgaccga aatggaaaaa tacccagatg aacgccgtaa gaaagccgaa tatctgattg
      601 caacgcaatc agttgccaat gccggcatgc agcttttact ggcagcgcat gcagaaggct
      661 tgggcgccgt atggatatgc agcccattat ttgctcaatc tgctgtgcaa gaagcattaa
      721 gacttcaacc cacatgggaa cctcaagcta tgttcctggt cggtaaacca gtagaggttc
      781 cggcggtgcg agaacgcaaa ccgatcgaag aaatttcgat cttcattgat aaaaagggaa
      841 attaaattga aaatcgtagc atttgcaggc ggggttggcg gagcaaaact ggcacaagga
      901 ctcgctcaaa cccttccacc aaaagactta accattatcg tcaacactgg tgatgacttt
      961 gaacatttgg ggctttatat ctgcccagac cttgataccg tttgttacac cttgggcggg
     1021 cttgccaacc ccgagaccgg ttggggtcgg gctggcgaga cgtggaacac catagcaaac
     1081 atcgaaaagc ttggcggacc ggcatggttt cgccttggcg actcggatat tgcaacccat
     1141 cttgagcgca cgcgacgact aaaagaaggg aagtctcttt cagagatcac aaaagaattt
     1201 tgcaaagctt ggggcattga agcgacagtg ttgcccatga cggattcgcc tgtgcgaacc
     1261 atggtcgact ctgatgaagg cgaattggca tttcaagaat atttcgtcca cagacaatgc
     1321 caaccgagag tgaaaggctt caggttcgac ggagtcgaag tcgctgaacc agctcagggc
     1381 gtgcgtgagg cgcttgcgtc tgcagatgcg atcattttct gcccatccaa tccgtgggtc
     1441 agtattgatc ctattctgag cgttgtcaaa gaagtgaata aaccggtttt tgcggtttcc
     1501 cccatcattg gtggaaagac ggtgaaagga ccggcagcaa agatgtatag tgaacttggt
     1561 attgaaccgt cagcttatgc ggttgccaaa cattatcgca ccatcttaaa aggatttgtg
     1621 ttagacacta cagatgctgc catggaaaaa gaaattcaag accttggctt tgcaactctt
     1681 gtcacaaaca cacttatgaa tcagcttaca gatcgcgcac gactggcgaa cgatgtgcta
     1741 aactttatcc ggagtttata aaaatgtcac tttgggcaat tgtgcctgta aaaccattgc
     1801 gccgcggtaa gtcccgcctt gccggggcat taactgaaga agaacgcgcg gcattgaatc
     1861 aagaattact ggaacgcact ctcaaaaccc ttaccagttt aaaagaatta gacaaggtac
     1921 tggtcgttag ccgcgactca catgcattga ccattgcacg taatcatggc gctaaaaccg
     1981 ttcaggaaga tggtcaaccg catttgaaca cagcgttaac gcgtgcatct gtgatcgcaa
     2041 aggttcatgc aactcaaggc gtgttggttt tgcccgtgga tcttcccctg cttacaactg
     2101 atgatgtttt agctttgatc gaccgagcta ccaagccacc cgttgtggtc attgcaccgg
     2161 atagacatca taaagggaca aacgctttgc tcatggttcc ggctggacag atcgaatatg
     2221 actttggtga agggtctttt caaaggcata gcgagcgagc gcttcaatct ggcgcaaggt
     2281 tagaagtggt tgaacttcca tcgcttgggt tagacttgga tgaccctgaa gacttggaac
     2341 tcattcgcaa gttggaagca caaaaagtat agacatcagt tttgccacat gctcgcaaac
     2401 cctatgaaat ttgcgaaaca tgtggcaaaa ttttttatat tgtcagacaa tcaagtcgta
     2461 tcattccaca ctcgaaggag aatgtcatga ccaagcaacg tgttgctctg tacttgcaag
     2521 actcgcacga cctacgtgat gggctcgact atgcaaagta tgccgaagaa aaagggtttg
     2581 aagccgtatg gcaagcggag agtcgcttag tgcgcgatgc gatcgtgccg atggcagggt
     2641 atgctgctgt aaccaacaag atcaaagtcg gctcgggtgt gatcaataac tggactcgca
     2701 acattggtct attggctgcc accttcttaa ctttggatga tcttgctccc aaccgtgtca
     2761 tttgcgggct cggcgcatgg tgggacccac tagctgcgaa tgtaggcatt gaccgcaaga
     2821 agcccttgac tgccatgaaa gaaaccgtgc aggtcatgaa gaaattgctc aacatggaac
     2881 gtgtcacctt tgatggggaa ttcattcatg taaaaggtat tgaattggac gtggtatatg
     2941 gacgacggga accgcgcaac attcccatca tgatcggtgc aacgggcgat aagatgatgg
     3001 aattgaccgg cgaaattgca gacggtgctg tgctcaatta ctgcgttgcc cctgaataca
     3061 atgacaacgc catggaattg ctgaataatg gcttgaagaa atctggtcgt aagatggaag
     3121 actttgaccg tcc
//
LOCUS       DAOREF010000310         3125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20458_length_3125_cov_4.682736, whole genome shotgun sequence.
ACCESSION   DAOREF010000310 DAOREF010000000
VERSION     DAOREF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20458_length_3125_cov_4.682736"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..623
                     /locus_tag="PKI33_07520"
     CDS             168..623
                     /locus_tag="PKI33_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNM36893.1"
                     /translation="MIIDRIENIALYVSLHPRFKQAFDYLSQIDIHAISVGRHDVDGD
                     AIYALVQEYTTKQTGEGKWEAHRRYIDLQYVVQGAEGMGYANIHHLQQGGYVPEKDFL
                     PLFGEGDQVPLRSGNFVLLFPEDAHMPSMALGKPAPARKIVLKIEVEAR"
     gene            complement(650..2254)
                     /locus_tag="PKI33_07525"
     CDS             complement(650..2254)
                     /locus_tag="PKI33_07525"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36894.1"
                     /translation="MKEFFTQRIKRGLVRRLTLLKVASVAREVARKEPQPNGAPIVFF
                     KASTGIDDLSWNSGFHLLTTWALRLQGIPVAYFACNSGLSKCVLGTNRDNPHKEMPCK
                     SCVMQSKALYTGVEGHKSKVGWFDFQRDPELVSRISSLSVSELSTFHFQDLPLGELCL
                     PALRWILRIHHLNDDENTRYLLREYILSAWNVAQKFSKFLDETSPRLVVVFNGQFYPE
                     ATARYLAKKRGLRVITHEVGLEPATAYFTEGEATAYPIHIPDEFELSEAQNARLDAYL
                     AKRFKGDFTMAGVKFWADMKGLDEAFLQKAAQFKQIVPIFTNVIFDTSQPHSNTVFED
                     MFAWLDTTLEVIKEHPETLFIIRAHPDELRLRKASRETVGGWVESTRVTSLPNVVFVP
                     PNETLSSYELIAKSKFVMIYNSTIGLEASIMGAAVLCAGKARFTQYPTVFFPQTVEDV
                     RRKMKEFLTAETIKVPVEFKRNARRFLYYQLYRTSLPLGQFLEPSVRVTQTKLKTFDL
                     AELQKSKSMQAILDGLLNNGDFLLKE"
     gene            complement(2325..>3125)
                     /locus_tag="PKI33_07530"
     CDS             complement(2325..>3125)
                     /locus_tag="PKI33_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNM36895.1"
                     /translation="QAIWHWKRFSFNDAPPIFANSKPKSGSHLLLQVMNGFTQIMPYK
                     YVDADPVRTITYKGRRKTNEEILADLKHTPKGVIGWGYVDATPENASFLTSAGRVNYF
                     IYRDPRDMLVSQVFFATDMHEEHGMHDYYNSLPDFGARLNVAITGIDRDRLKMVSVKQ
                     RYEGVFQWLEQKNTMCLRFEDLINNRDATLLKMLDEVEKTGYKIPTPREKALPILVEA
                     IQPKKSHTFRSGKTGGWAQHFTAEHKKLFKDVAGDLLVRLGYEKNNDW"
BASE COUNT          842 a          715 c          758 g          810 t
ORIGIN      
        1 cggcaagggt ggatggcatt caaaagattg cacatgtggg tcagatattt cagccaggtg
       61 atgatattga aacagatatg aaagcgattc aggaatcttt cattggcatg acgggcatca
      121 atccacgcct aaagacaaag aaaaaatata ttacgttggc aaattaaatg atcatagacc
      181 gaattgaaaa cattgcccta tatgtttcgc ttcatccgcg tttcaagcag gcatttgatt
      241 acttgagcca gatcgatatt catgcgatat ccgttgggcg gcatgatgta gatggagacg
      301 ccatctacgc tttggttcag gaatacacga ccaaacaaac aggggaagga aaatgggaag
      361 ctcatcgccg ctatatcgac ttgcaatatg tggtgcaagg cgcagaaggc atgggctacg
      421 ctaatatcca tcatctacaa caagggggat atgtacctga gaaggacttt ctgcctttat
      481 ttggcgaagg tgatcaagtg ccgttaagaa gtggtaactt tgtattgcta ttccccgaag
      541 atgctcatat gcccagtatg gcattgggta agcctgcacc tgctagaaag attgttttga
      601 aaattgaagt agaggcgagg taataccact tcgtggcaca aaccccgccc tactctttta
      661 gtaagaaatc cccgttattc aacaagccat ctaagattgc ctgcatagac ttagatttct
      721 gtagctcagc caaatcaaac gtctttaact tcgtttgagt cacacgcacc gacggctcaa
      781 gaaattgacc taagggtaaa gaggttcgat ataactgata atacaagaag cgacgtgcgt
      841 tgcgtttgaa ttcgacaggg actttgatcg tctcagccgt caaaaactct ttcatctttc
      901 ttcgtacatc ctctactgtt tgcgggaaga atacggtggg atattgagta aaacgcgcct
      961 taccagcaca caagaccgca gcccccataa tagaagcttc caagccaatg gtcgagttat
     1021 agatcatgac aaatttagat ttcgcgatta attcatagga acttagcgtt tcgtttggcg
     1081 gaacaaaaac aacattcggc aaactggtga ctcgggtaga ttccacccat ccgcccactg
     1141 tttcacggct ggctttgcga agacgtagct catcggggtg agcgcggata atgaacaagg
     1201 tctctgggtg ttctttgatg acctccagag tcgtatctaa ccaagcaaac atatcttcaa
     1261 agaccgtgtt ggaatgtggt tggctggtat caaagataac attagtaaag atgggcacga
     1321 tttgtttgaa ctgagccgct ttttgaagga aggcttcgtc cagtcctttc atatcagccc
     1381 agaatttgac gcccgccatc gtaaagtcac ctttgaagcg tttggcaaga taagcatcga
     1441 gccgtgcatt ttgcgcctcg cttagctcaa attcatcggg gatatgaatc gggtaggctg
     1501 tcgcttcgcc ttcggtgaaa taggcagtag caggctccaa gcccacctca tgggtgatga
     1561 cgcgcagtcc gcgttttttg gcgagatacc gtgccgtcgc ttcggggtag aactgtccat
     1621 tgaagaccac caccaaacgt ggacttgtct catcgagaaa tttcgagaac ttttgcgcca
     1681 cattccatgc ggacaaaatg tattcacgca aaagataacg cgtgttttcg tcgtcattca
     1741 agtgatgaat acgaaggatc cagcgcaggg cgggcaagca caattcaccg agaggaaggt
     1801 cttgaaaatg gaaagtggaa agttcagaga cagaaagtga tgagatacga gaaacgagtt
     1861 caggatcacg ctgaaaatca aaccaaccga cttttgactt gtgaccttcg actcctgtat
     1921 aaagcgcctt gctctgcatc acacaagatt tacaaggcat ttctttatgg ggattatcgc
     1981 ggttcgtacc caatacgcat ttgctcagcc ctgagttaca agcaaaatat gccacaggga
     2041 tgccctgcaa gcgcaaagcc caagtggtca acaaatggaa cccactgttc catgagaggt
     2101 catcaatgcc agtggaggct ttgaagaata caattggtgc gccatttggt tgcggctctt
     2161 tacgagccac ctctcgcgca acggatgcaa ctttgaggag ggtgagtctg cggacgagtc
     2221 cgcgtttgat gcgttgagtg aagaattctt tcatagtcgt gcaaattgta tcagagagca
     2281 gtacataaaa aatgggactg aatttctcca gtcccatgca tatactacca gtcgttgttc
     2341 ttttcgtacc ctaacctgac cagcaaatct cctgctacat ccttgaaaag cttcttatgt
     2401 tctgcggtga agtgctgagc ccaaccgccg gttttgcccg agcggaaggt gtgacttttc
     2461 tttggctgaa tggcttccac caaaataggt aatgcttttt cacgcggagt ggggatcttg
     2521 tagccggtct tttccacttc atcgagcatt ttcaacaagg tcgcatcgcg gttgttgatg
     2581 aggtcttcaa aacgaagaca catcgtattt ttttgctcca accactggaa gacgccttca
     2641 tacctctgct tcacgctaac cattttcagc ctgtcacggt caatgccggt gatggcaaca
     2701 ttcaaacgtg cgccaaaatc aggcagggaa ttgtagtaat cgtgcatgcc atgttcttcg
     2761 tgcatatcgg tcgcgaagaa gacttgtgag acgagcatat ctctcgggtc gcgataaata
     2821 aaatagttca cgcgcccggc tgaggtcaag aaagaggcgt tttctggggt ggcatccaca
     2881 tagccccaac cgatcactcc ctttggggtg tgcttcagat ccgcaagaat ttcttcgttt
     2941 gtttttcttc tgcctttgta ggtgatcgtt cgcacggggt cggcatccac atacttgtag
     3001 ggcatgatct gcgtgaagcc attcatcacc tgcaaaagca aatgcgaacc gctcttgggc
     3061 tttgagttag caaagatggg cggtgcatcg ttaaacgaaa accgcttcca atgccagatg
     3121 gcttg
//
LOCUS       DAOREF010000311         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20476_length_3123_cov_5.306389, whole genome shotgun sequence.
ACCESSION   DAOREF010000311 DAOREF010000000
VERSION     DAOREF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20476_length_3123_cov_5.306389"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="prmA"
                     /locus_tag="PKI33_07535"
     CDS             complement(<1..756)
                     /gene="prmA"
                     /locus_tag="PKI33_07535"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNM36896.1"
                     /translation="MNWLEVSLTVNGELAESVADVLARFAPNGVMTEQGVRFVNDEDE
                     GTATGPITVRAYLEVNDQLEETRQKIEESLYYLGMITPVPTPTYKQIADQNWMEAWKQ
                     HYKPILIGEGLLILPAWLDNPDPKRIPIKIDPGMAFGTGTHPTTQLCLELMERSVNSN
                     WRLVIDVGCGSGILSIAALKRGAEKVLGVDIDIESVKNSRENADLNGIGEELTLGQGS
                     VTEVLAGQFAFKSAPLVVANILAPVLIRLFDAGL"
     gene            complement(760..1239)
                     /locus_tag="PKI33_07540"
     CDS             complement(760..1239)
                     /locus_tag="PKI33_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNM36897.1"
                     /translation="MESRKYPVHIVAAGGFVINSENQVLMLKSPRYDDWEFPGGQIEE
                     GEIIPKGLEREVLEETGIVVRVKNLIGIYSNIGNPPIVNMDFLCEYISGEPKTSNESI
                     DVKWVDRNEVLNLVERKVIRTRLQNMLEFSGEINYLAYSVNPNIKDLSYQEFENRKI"
     gene            complement(1242..1625)
                     /gene="crcB"
                     /locus_tag="PKI33_07545"
     CDS             complement(1242..1625)
                     /gene="crcB"
                     /locus_tag="PKI33_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNM36898.1"
                     /translation="MTNILLVGAGGFIGSVLRYLVSGYIQQSANRMDFPFGTLAVNII
                     GCFIIGLLAQFGDKYGTFSNESRAFLVIGVLGGFTTFSSFGNDTVNLIRQDFVMNAVA
                     NVGANVIIGLLAVWLGRTVGQLIWR"
     gene            complement(1622..2167)
                     /locus_tag="PKI33_07550"
     CDS             complement(1622..2167)
                     /locus_tag="PKI33_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNM36899.1"
                     /translation="MDESLLVFVLTADGELAVSGLSSAVVYNDSPMLKPLFRLWRLLP
                     MWVHVIVTRITQPKFNAGVSSLVFDEHGRVLLFKHTYRKYEWGIPGGALEYREQPIEA
                     VVREFYEETSMQIEVIKLLRVASAREFPHLGITYLCKITGGEFKPSHEISEMKYFDVN
                     DLPIMLFAEKDLIRWAVEESK"
     gene            complement(2200..>3123)
                     /locus_tag="PKI33_07555"
     CDS             complement(2200..>3123)
                     /locus_tag="PKI33_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNM36900.1"
                     /translation="FGKAGVSGAGGQGYQDFSGFGDIFEDLFNGFGFSTGGGRSRRSP
                     RRGRDLQMQVSLTFEEAVFGVEKEIEFSRDETCSRCSGKGAEPGTTPLRCSTCNGQGE
                     VRQVRQTFLGQMVQTAPCPTCNGRGETIASPCTTCRGNGLERKKVKKKVSIPAGVDMG
                     TQIRLNGEGAPGENGGPQGSLFLVLDVKPHKFFKRRDQDVILNLDINIAQAVLGAEVT
                     IPTLDGEDRLKIPSGTQPGKVFHVKGKGVPHVRNKNQRGDEIVIVNVAVPTKLSKEQK
                     ELFEKLAESLGTTVKPQEKGFLDWLNDAFGG"
BASE COUNT          777 a          905 c          665 g          776 t
ORIGIN      
        1 cagccctgca tcaaagagac ggatgagcac cggcgcgagg atgttcgcca ccaccagcgg
       61 cgcggacttg aacgcaaact gccccgccag cacctcggtc acggaaccct gcccgagggt
      121 caactcctcc cctattccat tcaagtctgc attctcacgg gagtttttta cggactcgat
      181 gtcaatgtca acccccaata ccttttctgc gccacgtttc aacgccgcaa ttgaaagaat
      241 acctgagcca caccccacat caattaccaa tctccaatta ctatttaccg atctctccat
      301 caactccaaa cataattgcg tcgtcggatg agtacctgtg ccaaacgcca tgccggggtc
      361 gatcttgatc gggattcgtt ttgggtcggg gttgtccaac caggcaggga ggatgaggag
      421 cccttcaccg atgagaatcg gcttgtagtg ttgcttccag gcttccatcc agttttgatc
      481 cgcgatctgc ttgtaggtgg gagtgggtac cggtgtgatc atccccaaat aatagagcga
      541 ttcctctatt ttctgtctcg tctcttcgag ttgatcgttg acctccaaat atgctcggac
      601 ggtgatggga cccgtggcag tgccctcgtc ttcatcgttc acaaagcgga caccctgctc
      661 ggtcatcacg ccgttgggcg caaaacgtgc cagcacatcg gcaacagatt cggcgagttc
      721 gccgttgacg gtcaaagaaa cttcaagcca gttcatgttc tatattttcc tattctcgaa
      781 ttcttgataa ctcaggtctt tgatatttgg gttcacacta taggcgagat aattaatttc
      841 gccagaaaat tcaagcatat tttgcaatcg tgtgcgaatg acttttcgct ctaccaagtt
      901 taacacttca tttcgatcaa cccattttac gtcaatgctt tcgttgctgg ttttgggttc
      961 acccgaaata tattcgcaca gaaaatccat gttgacgatt ggcgggttac caatgttcga
     1021 atagatccct atgaggtttt taactctgac tacaattcct gtttcttcca acacttcccg
     1081 ttctaaacct ttaggaatga tctcgccttc ttcaatctgc ccgccaggaa attcccaatc
     1141 gtcgtagcgt ggacttttca gcatcaaaac ctgattctcg ctgttgatga caaatccgcc
     1201 tgccgccacg atatgaactg gatattttct actttccaca cttatctcca gattaattgt
     1261 ccaacggttc gacccagcca aaccgcgagt agtccgatta tcacattcgc gccaacattt
     1321 gcgacggcat tcatgacaaa gtcttgcctt atcaagttga ccgtgtcatt gccaaaggac
     1381 gaaaaggtgg tgaagccgcc taagacgcca atgaccaaaa aggcacgaga ctcatttgag
     1441 aacgtgccat atttatcgcc aaactgcgcc agcagaccaa tgataaagca gccgatgata
     1501 ttgaccgcca atgtaccgaa cggaaagtcc attcgattcg cagattgctg aatatagccg
     1561 ctgaccaaat aacgcaagac cgagccgata aatccgcctg cgccgacaag aagaatgttt
     1621 gtcacttgct ttcctccact gcccagcgga tcaaatcctt ctccgcgaac agcatgatgg
     1681 gcaagtcgtt cacatcgaag tatttcatct ccgaaatctc atgcgacggt ttgaactcac
     1741 cgcccgttat tttacacagg taggtgattc ccaaatgcgg aaattccctc gcacttgcca
     1801 cccgtaaaag tttaatcacc tcgatctgca tgctcgtctc ttcgtaaaac tcacggacta
     1861 ccgcttcaat cggctgctcc ctatattcca acgccccgcc gggaatcccc cactcatact
     1921 tgcggtaggt gtgtttgaaa agtaacactc tgccatgctc gtcaaagacc aacgaagaga
     1981 cacccgcatt gaattttggt tgagtaattc tggtaacaat aacatgaacc cacatgggta
     2041 gcagacgcca aagacgaaat aggggtttaa gcataggcga gtcattataa acgaccgcag
     2101 atgatagacc gctgaccgcc aattcaccat ctgccgtcaa aacaaaaacg agcagacttt
     2161 catccactcg tttttaatta ccaatctcca attaccgccc tatcccccaa acgcatcatt
     2221 caaccaatcc aggaacccct tctcctgcgg cttgacggtc gtgcccaaac tctctgccaa
     2281 tttctcaaag agttctttct gttccttcga caacttggtt gggacagcca cattgacgat
     2341 cacaatttca tcgccgcgct gattcttatt gcggacatgc ggcacgccct tgcccttcac
     2401 atgaaagact tttccgggct gtgtgccaga gggaatcttc aatctatctt cgccatcaag
     2461 agtcgggata gttacttccg cacctaagac cgcttgtgcg atattgatat cgaggttcag
     2521 aattacatcc tgatcgcggc gcttgaagaa cttatgcggc ttcacgtcca acacgaggaa
     2581 caaactgccc tgcggaccgc cgttctcgcc gggtgcccct tcgccattca agcggatctg
     2641 cgtgcccatg tccacacctg cggggatgga tactttcttc ttgaccttct tgcgttccaa
     2701 gccgttgccg cgacaagtgg tacacggcga agcaatcgtc tcaccgcgac cattacaggt
     2761 tgggcatgga gccgtctgca ccatctgacc taaaaaggtc tgacgcacct gacgtacttc
     2821 gccctgccca ttacaagtgg aacatcttaa cggagttgtc cccggctcag cacctttgcc
     2881 actacaacgt gaacaagtct catctcgtga gaactcgatc tctttctcaa cgccgaagac
     2941 cgcttcctca aaggtaagcg aaacctgcat ttgtagatcc cgcccacggc gcggagagcg
     3001 gcgcgaacgt cctccaccgg ttgagaatcc aaatccgttg aacagatcct caaagatgtc
     3061 gccgaagcca gagaaatcct gatagccctg cccgccagca ccgctgacac ccgccttgcc
     3121 aaa
//
LOCUS       DAOREF010000312         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20691_length_3106_cov_3.012783, whole genome shotgun sequence.
ACCESSION   DAOREF010000312 DAOREF010000000
VERSION     DAOREF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20691_length_3106_cov_3.012783"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1391
                     /locus_tag="PKI33_07560"
     CDS             174..1391
                     /locus_tag="PKI33_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNM36901.1"
                     /translation="MESIETIIIGSGQAGLATSYHLKQLGREHIIFEAADIPAHVWRD
                     DRWDSFTFVTPNWAIQLPGAGYEGRDPDGFLKKDEILAYFEGYVEKYQFPIQLNTTVT
                     EVAPVEGGYQVIANGKTYHAKNIVMATGSFQKPKIPAYSANIPAGVLQLHSGHYRNPS
                     QLPAGAILVVGAAQSGMQIAEELYQSGRTVYLATGFAPRAPRRYRGRETFSWLNETGF
                     VSTPVEKLPSPKARFISNPQLSGKDGGHSLNLHQFARDGVKLLGHIAGAQDGKVMFKA
                     DLKENLAKTDKTEKDILAMIDKYIEANGINAPQETIPELQDGYASEEITELDLKAENI
                     NTVIWAIGYTADYSLVKLPITDEDGFPITQRGITQYPGLYFVGMHWLSSRKSTILLGV
                     NEDVEHVIADMTK"
     gene            1605..2738
                     /locus_tag="PKI33_07565"
     CDS             1605..2738
                     /locus_tag="PKI33_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM36902.1"
                     /translation="MTKKRVVVLGAGFGGLELSTILSDALGDELDLTLIDRNDSFYFG
                     FSKFDVMFGNKTPDAVRIAYRNIDKRGVIFRQETVKAIDPVTRRVTTDKATYEADVLV
                     IALGADYDIAHTPGLAEAGNEFYSFAGAERLRSVLPAFNKGRVIVGVTSTPFKCPPAP
                     SEAALLTHDYLTERGIRADCEISLVMPFGIPIPPSPETSAALMAAFKERGIRFVPNRL
                     VRALDGARRVAILDNGDEMPYDLFLGVPKHRVPDVVAESGMTEDGWIPVDTKTLKTRF
                     ENVYAIGDVNSVGTPKAGVFAEGAARTVAAEIIADMKGGHADAYRGIGACYIEFGAKR
                     VGKVQVEFLAGPPTGSYTEASSALVDEKQEFGASRRAKWFGLG"
BASE COUNT          848 a          750 c          789 g          719 t
ORIGIN      
        1 gtccgattac gaaaccgcga tgatggaaaa attcggcaag gatttgaccc cgcacagagt
       61 caaatacaga acgttgaaac ggtaaaccaa agtgactgtc acccacaagg tgacagtcac
      121 ttttttatct gcctcgtgtt gtaaccatca tattatcggt aaaggagcac ggcatggaat
      181 caatcgaaac catcatcatc ggcagcgggc aggcgggact agccaccagt taccatttga
      241 aacaacttgg acgtgaacac atcatcttcg aagcggcgga tattcccgcc catgtctggc
      301 gcgatgaccg ctgggactca ttcacctttg tcacccccaa ctgggcgatc caactccctg
      361 gtgctggtta tgaagggcgt gaccctgatg gctttctaaa aaaagatgag atccttgcct
      421 attttgaagg ctatgtcgaa aaatatcaat tccctattca attaaacaca accgtgaccg
      481 aagtcgcacc cgtcgaaggc gggtatcaag tcattgccaa tggaaagacc taccacgcta
      541 aaaatatagt gatggcaaca ggttcctttc agaagccgaa gattcccgcg tacagcgcaa
      601 acatcccagc aggtgtattg caactccatt caggtcatta ccgcaatccg agtcaactgc
      661 ctgcgggcgc tatccttgtc gtcggcgcgg cacagtcggg catgcagatc gccgaagagc
      721 tatatcaaag cggacgcacg gtctatctcg cgacaggttt tgcgccgcgc gcgccgcgcc
      781 gttatcgcgg cagggaaacg ttttcatggc ttaatgaaac ggggttcgtt tccacacctg
      841 tggaaaaact cccgtcccct aaagcccgtt ttatcagcaa tccgcagctc agtgggaaag
      901 acggtggtca ttccctcaac ctgcatcagt ttgcgcgtga cggcgtgaag ctcctcggtc
      961 acattgcagg cgcgcaggat ggcaaggtca tgttcaaagc cgacttgaaa gaaaaccttg
     1021 cgaaaaccga caaaaccgaa aaagacattc ttgccatgat cgataaatat attgaagcga
     1081 acggcatcaa cgccccgcaa gaaaccatcc ctgaattgca ggatggctat gcctctgaag
     1141 agatcaccga actcgacctg aaagcagaga acatcaacac cgtcatctgg gcgatcggct
     1201 acaccgccga ttacagcctc gtgaaattgc ccatcaccga tgaagacggc ttccccatca
     1261 cccagcgcgg catcacgcaa taccctggct tgtactttgt cggtatgcac tggctcagtt
     1321 cacgtaaatc cacgatcttg ctgggcgtaa atgaggatgt ggagcatgtg attgcagata
     1381 tgacaaaatg aagcggagaa attcggcaag gatctaactc cgcatcgggt caaatataga
     1441 acgatgatga gataattcgc cccgcccctg cattcggtta cgcaaccatg atgagggaaa
     1501 agttcggctc tatgttggaa gcaaaaaaca ttggattttc tgcaacggca taagggtaca
     1561 atatcgctta ttgaagacca tttattaata acaaggagag atttatgaca aagaaacgag
     1621 tggtggtgtt aggcgctggg tttggcggat tggaattgag tacgatcttg tccgatgctc
     1681 tgggtgacga gctggacctg acattgatcg acaggaatga ttctttttat tttggctttt
     1741 caaaatttga cgtaatgttc ggcaacaaaa cacccgatgc ggtacgcatt gcatatcgca
     1801 atatcgacaa gcgtggcgtg atctttcggc aggaaacggt caaagccatt gaccctgtaa
     1861 cgcggcgtgt gacgaccgat aaagcaacat acgaagccga tgtgctggtc atcgctttgg
     1921 gcgccgatta cgacattgcc cacacacctg gccttgcaga agcgggcaat gagttctatt
     1981 cgtttgctgg agcagagcgg ttgcgcagtg tactgcctgc gttcaacaaa ggacgcgtca
     2041 ttgtgggcgt tacctcaacc ccattcaaat gtccacctgc gccgagtgaa gctgccttat
     2101 tgacacatga ttatttgaca gaacgcggta tacgcgcaga ttgcgagatc agtctggtca
     2161 tgccgtttgg cattccgatc ccgccctcgc ccgaaacgtc agcggcgcta atggctgcat
     2221 tcaaagagag aggcatccgc ttcgttccga atcggctcgt ccgcgcattg gatggtgccc
     2281 gacgggttgc cattctcgat aacggggatg aaatgccata cgatctcttc ctgggcgttc
     2341 ccaaacatcg tgtgccagat gtggtcgccg aaagtggaat gacggaagat ggttggattc
     2401 ccgtggatac aaagacattg aagacgcgtt tcgaaaacgt gtatgccatt ggcgatgtga
     2461 acagtgttgg gacacccaaa gcaggcgtat tcgccgaagg cgcagcgcgg acggttgctg
     2521 ctgagatcat cgctgatatg aaaggcggtc atgccgatgc ctatcgcggc ataggggcat
     2581 gttacattga gtttggagca aaacgtgtgg gtaaagtgca ggtggaattc cttgctggtc
     2641 cacccacagg aagttacacc gaagcatcgt ccgccctggt ggatgagaag caggaattcg
     2701 gcgcaagccg cagggcgaag tggtttgggc tgggataaga ttcatctggt ccgattacga
     2761 gaccatgatg gaagaaaaat tcggcaagga tctaaccccg tatcgggtga agtacagaac
     2821 gttgaagaaa tagtcagaaa atacatagag caaaaaaaga gacagtcatt caaagatggc
     2881 tgtctctttt tttgcaggtc aaaaaactac cccagcgttg tttccacaat ttctctcgta
     2941 ctacttcata ctttctccac atttaataag taaattaggg cgaaaaatac aaacagaggt
     3001 aaaaatgaaa tctttttcaa cacaaaatac aaataaattt ctaaagcaat tggcatggtg
     3061 ggtcttgttt ctgatcagtt cattttatgg attgtatgca ttctat
//
LOCUS       DAOREF010000313         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20695_length_3105_cov_5.199016, whole genome shotgun sequence.
ACCESSION   DAOREF010000313 DAOREF010000000
VERSION     DAOREF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20695_length_3105_cov_5.199016"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PKI33_07570"
     CDS             complement(<1..1050)
                     /locus_tag="PKI33_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNM36903.1"
                     /translation="MAYKIKFGTDGWRGVIAEDYTFDNLRRCAQGFASYMLSQGKKGQ
                     WVVVGHDKRFHSENFAAAVAEVLAGNGLKVYLTDGATPTPVIAYAVVDKKAAGAVNIT
                     ASHNPPTDNGFKVRNETGGAIDPEGLKQIEAGIPDDLKDVKLKNYKEAEAAGEIVKFD
                     AATPYIEHLTRDGLIDLQPIKDAGLTVVVDAMWGNGAGWFTRLLGGGKTKIIEIHNER
                     NPSFPEMKRPEPIRPNIDVGLKATVTNKADVLLITDGDADRCGLGDENGEFINQLRAY
                     ALLAYYLLEVRGERGDIVKTLSTTGMLNKLGEIYGVPVHETGVGFKYVAPKMTETNAL
                     IGGEESGGYAFRGNVP"
     gene            complement(1149..2177)
                     /locus_tag="PKI33_07575"
     CDS             complement(1149..2177)
                     /locus_tag="PKI33_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNM36904.1"
                     /translation="MSRFLDFLNTADLNTLTQVSGVNRQLAGNLIAARPFDAEEDALN
                     VKGMGKATLARLQSFAEAQGNGSESSAMVPVQEEAMPAVMEKNPPAQEDAPKKESFFV
                     RLGRAIRSFFVALLKLIMLAILFVSIGAMFYYGLPYIQRTFIAPVEQNTAQIKTMEAE
                     IETLQTQLDEMNSRVSALEGSVEAHTASIEKLEEMQTALETQLEENQDAVLLELKQEI
                     MFTRALDVLARARLYLAQSNFGLAKTDVQTARDLLAELNAEKEDEVLTKAIERIDLAL
                     DNLPDFPVVASGDLEIAWQILVSGNAPASTATATPTLVPTEPATATPTAVDTAATPTL
                     IPSATATP"
     gene            complement(2279..>3105)
                     /locus_tag="PKI33_07580"
     CDS             complement(2279..>3105)
                     /locus_tag="PKI33_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36905.1"
                     /translation="PRLALAGLFFVFSIGSRTTTLPGIALISITLLLWAYQTQRPRMF
                     PILLAFALPVAIGGIAYATYNYARFGSFTEFGYRYQLTSYNLYQKMDETFAIEYIPPN
                     LYKTLFNPLETREAFPHIFPTRWSGPEWLTNYHPKMYLTLTENITGVFVSSPFVLLAL
                     LAFFKPRRDVRWVIASIALCFLGIFITLQAFFFVAMRYMLDVIPTLSLLVIIGFWHGF
                     ELFKNKKVYIIITTLLLIHTIACSLLISFSGNLETFKILNPELVREMTWTFNNLLK"
BASE COUNT          790 a          755 c          784 g          776 t
ORIGIN      
        1 gggcacgttc ccacggaagg catagccgcc gctttcttca ccgccgatga gtgcattggt
       61 ctcggtcatc ttcggggcaa catacttgaa gcctacgccc gtctcatgga caggtacacc
      121 gtatatctcg cccagtttgt tcaacatacc agttgttgaa agcgtcttga cgatgtcgcc
      181 gcgttcaccg cgcacttcga gcaaataata cgccagcaac gcataggcgc gtaattggtt
      241 gatgaattca ccgttctcgt cccccaagcc acagcggtct gcgtcaccat cggtgatgag
      301 caacacatcg gctttgttgg tcacagtcgc tttcaaccct acatcaatgt tcgggcggat
      361 gggttccggg cgcttcattt cagggaagga agggttgcgc tcattgtgaa tttcaatgat
      421 cttggtcttg ccgccaccaa gcagacgggt gaaccaacct gcaccattgc cccacatggc
      481 atccaccacc acggtcagac cggcatcttt gatgggctgt aaatcgatca atccatcgcg
      541 agtgaggtgt tcaatatacg gagtcgccgc atcgaacttg acgatctcgc cagcggcttc
      601 ggcttcttta taatttttga gctttacatc cttcaaatca tcaggaatac ctgcttcgat
      661 ttgtttcaaa ccttcagggt caatagcgcc accggtctca ttgcggacct tgaagccgtt
      721 atcagtgggt gggttatgac tcgccgtgat gttcaccgca ccggcagcct tcttatccac
      781 cacggcataa gcaatgaccg gggttggagt cgcaccgtca gttagataga ccttcaagcc
      841 gttgccagcc aacacttctg ccacagctgc agcaaaattt tcggagtgga aacgcttgtc
      901 atgcccaact acgacccact gtcccttttt accttgtgaa agcatatacg acgcaaaccc
      961 ctgcgcacag cgacgcaggt tgtcgaacgt gtaatcttca gcaatgactc cgcgccaacc
     1021 gtctgtgccg aatttgattt tgtatgccat gagaattctc ctcttgggaa tgataacttt
     1081 cgaaattata cccgccacga gcctaaatat cataaaaaaa ctccgaggca ttgcctcgga
     1141 gttttgcgtt acggggttgc cgttgcagat ggaattaatg taggcgtagc cgccgtatct
     1201 actgctgtgg gagtggcagt tgctggctct gtggggacta gcgtgggtgt cgccgttgca
     1261 gtggatgcgg gtgcattccc agagaccaaa atctgccagg caatttccaa atctcctgag
     1321 gcaacgacag ggaagtctgg caggttgtca agcgcaaggt cgatccgctc gatggctttg
     1381 gttagaacct catcttcttt ttcggcattg agttcagcca gcaaatctcg tgcggtttgc
     1441 acatcggtct ttgccaagcc aaaattgctt tgtgccaagt acagccgtgc acgtgctaga
     1501 acatccaacg cacgggtgaa catgatctct tgtttcagtt ccaacagaac cgcatcctga
     1561 ttttcttcga gttgagtttc cagcgcggtt tgcatctctt ccagtttttc aatcgaagcc
     1621 gtatgagctt ctaccgagcc ctcaagtgca gaaacgcgtg agttcatttc atcgagctga
     1681 gtttggagcg tttcgatctc tgcttccatg gttttaattt gtgccgtgtt ttgttctaca
     1741 ggcgcaatga aggtccgctg aatgtatggc aagccataat agaacattgc gccaatcgat
     1801 acgaacaaga tcgccaacat gatcagcttt aacaatgcta caaagaagga tcgaattgcc
     1861 cgccctaaac gcacgaagaa tgattctttc ttcggggcat cttcctgtgc aggcggattc
     1921 ttctccatta cggcgggcat cgcctcttcc tggaccggaa ccatggcact gctttctgaa
     1981 ccatttccct gcgcctcagc aaaagactgc aagcgcgcaa gggttgcttt gcccatgcct
     2041 ttaacgttga gtgcatcctc ttctgcatcg aaaggacgcg cggcaatgag attccctgcc
     2101 agttgacggt taacgccaga tacttgagtg agggtattca ggtctgcagt gttgagaaaa
     2161 tcgagaaagc ggctcatgga atctcctata cggtcattgt acaacaaaag gtttcagatg
     2221 cgaatcagct tgaggtaata gatgcaagcc cacgtcacgc acgaggcgtg gatatctgtt
     2281 atttgagaag gttgttgaaa gtccacgtca tctcgcggac gagttctggg ttgagaatct
     2341 tgaaggtttc caaattgcct gagaacgaaa tcaacaaact acatgcgatg gtatgaatca
     2401 ataaaagcgt agtgatgatg atatacactt tcttgttctt gaaaagttca aagccatgcc
     2461 aaaagccaat gataacgaga agggatagtg tcggaatgac atccaacatg tagcgcatgg
     2521 cgacgaagaa gaaggcttgt aaggtgataa agatgccaag aaaacataaa gcgatggatg
     2581 cgatgaccca acgtacgtct cgccgtggtt tgaaaaatgc tagcagagcc agcaaaacga
     2641 acggactgct cacgaataca ccggtgatgt tttcggtgag ggttaaatac atcttggggt
     2701 gataatttgt cagccattca ggacccgacc aacgagtagg gaagatgtgt ggaaatgcct
     2761 ctcttgtttc gagcgggttg aaaagggttt tatataaatt gggcggaata tattcaatgg
     2821 cgaaggtctc atccatcttt tgatacaggt tatagctggt gagttggtaa cgatagccaa
     2881 attcggtgaa agaaccgaaa cgagcatagt tatacgtggc atatgctatg ccgcctatgg
     2941 caacgggaag ggcaaatgcc agcaaaatcg gaaacatgcg tggacgttga gtttgatatg
     3001 cccacaatag aagtgtaata gagatgagag caataccggg taatgttgtt gtgcgcgacc
     3061 caatcgaaaa gacgaagaac aagccagcca gcgccaaccg cggaa
//
LOCUS       DAOREF010000314         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20696_length_3105_cov_4.430820, whole genome shotgun sequence.
ACCESSION   DAOREF010000314 DAOREF010000000
VERSION     DAOREF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20696_length_3105_cov_4.430820"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKI33_07585"
     CDS             complement(<1..994)
                     /locus_tag="PKI33_07585"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNM36906.1"
                     /translation="MKRYLLFTVFISGMTTLAAELAAGRLIGNVFGTSNLVWASIIGL
                     ILIYLTLGYFLGGKWADKDPSPLAMYRILAWGAFTVGLVPYVAVPVLRSAASAFEALS
                     VGIMAGSFIVVLILFIVPITLLGAISPYAIRLSVDDASKAGQISGQIYAVSTLGSFIG
                     TFLPTLVFIPTVGTRLTFAIFGMTLLVVALVGLARFASTRDMLKLIWMPILLAVIAFI
                     FSSSALKTNKGQVYETESEYNYIQVQESNGYTLLRLNDGQGIHSIYHPENLFYNGPWE
                     LFMVGPYFYENRSPDDIKEMAIIGLAAGTAARQATIVYGEDLHIDGYELDPKIAE"
     gene            complement(1085..2887)
                     /locus_tag="PKI33_07590"
     CDS             complement(1085..2887)
                     /locus_tag="PKI33_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNM36907.1"
                     /translation="MKTKSTNPLSLFFNLTVLAAYFHALMEWLFFVTQSSSLSTLSLL
                     DKIEVLFSTGGVIVLLLLALLVVFTVPALKWKFFGYVPAALMVSVTALVMIDNFTYTV
                     FKFGIVSTSGAWRLLYTVAFVLLFYWVFRFVERQKLWKGVSFLSLGLLGVSTVVILSI
                     YNSRSPYMSDFTPEATEASAEHPNILILGSDGLSASYLSAYGFEGNTTPFIAELTQTS
                     LVAENAFPNVSSTTGSTTSALTGKMPAEVQVFRYPDILSKEDSFEHLPGILKHMGYKT
                     VELGTTYYVDARKLNLADGFELVNNVSVDNPILDAIQPLLGNSPSAFFLQTVTERASD
                     RLFHIFFIKEMENPFLQVNNPNARMTDEERTQQIINLLETSDRPVFVFSHYMNTHGPL
                     FSTDDKHVEENEDSAESEKEWDVELYKKAIRTFDDHVREIYTYLEESGKLDNTIIVIY
                     TDHGYRYVVNQRIPIIFHFPNNDHAGTIQNNTQIIDIPATLLDYINVDQPEWMTGSSL
                     LLGEPPAMREIISTTAGSPKKIKPPFYQIKSLQFIVCQKWYSWNVQDNEFKTGEVKSH
                     TAKCESNLLPSDDEIRQRMIEYFKKYNYDTSSLE"
BASE COUNT          867 a          672 c          785 g          781 t
ORIGIN      
        1 cttcagcgat ctttgggtcg agttcgtagc cgtcaatgtg caggtcttcg ccatagacaa
       61 tcgtagcttg acgagccgcc gtccctgctg caagcccaat gatcgccatt tctttgatgt
      121 catctggcga acgattttca tagaagtatg gtcccaccat gaagagttcc catggaccgt
      181 tgtagaaaag attctcagga tgatagatgg agtgaatgcc ctgaccgtcg ttgaggcgca
      241 gtaaagtgta tccgttcgac tcttgaactt gaatatagtt gtattcagat tcggtttcgt
      301 acacctgccc tttattggtt ttcaatgcag aacttgaaaa gatgaatgca atcactgcca
      361 gcaaaatagg catccaaatt aatttgagca tgtcgcgagt gctggcaaaa cgagccagac
      421 ccaccagtgc aaccactaaa agcgtcatac cgaaaatggc aaaggtgagg cgtgtgccaa
      481 cggtgggaat aaaaacaaga gtgggcagaa aggttccaat gaacgaaccc aacgtagaca
      541 cagcatagat ctgcccggaa atttgaccag ctttgctggc atcatctacc gagagacgaa
      601 tggcgtatgg tgaaatagct ccgagcaaag tgatgggcac aataaagagg atgaggacaa
      661 cgatgaatga ccctgccata atgccaacgc ttaacgcctc aaacgcgctc gccgcggacc
      721 tgagaacggg tacggcgaca taaggcacaa gccccactgt aaacgctccc cacgccaaaa
      781 tgcgatacat tgctagtggg cttgggtctt tatctgccca tttgccgcca aggaagtatc
      841 ccaacgtcag gtagatgagg atcaagccga tgatgcttgc ccaaaccaga ttgctggtac
      901 caaacacatt accaatgagg cgccccgccg caagttcggc tgcaagcgtg gtcatgccag
      961 agatgaaaac cgtaaagagt aggtatcgtt tcatgaaaat ccctataaga agatgcgcgg
     1021 attatagcgt acttcatgca gtgagaaata agaactccct tagcacctaa gggagttctg
     1081 gatgttactc aagcgaactg gtatcgtagt tatatttctt gaaatattcg atcattcttt
     1141 gacgaatttc gtcatcagat ggcaagagat tcgactcgca ttttgcggtg tgacttttta
     1201 cctcgccagt tttgaattca ttgtcttgca cattccatga ataccatttc tgacagacaa
     1261 tgaattgcag acttttgatc tgatagaacg gtggtttgat ctttttgggt gaacccgctg
     1321 tggtactgat aatctctcgc atggcgggcg gttctcctag caagagcgat gagccggtca
     1381 tccactcggg ctggtcaacg ttgatatagt ccagcaaggt ggcgggtatg tcaatgatct
     1441 gcgtgttgtt ttggatcgtg ccggcatggt cgttgtttgg gaagtggaaa atgattggga
     1501 tgcgctggtt gaccacatag cgataaccgt ggtctgtgta gatgacgatg atggtattgt
     1561 ctaacttgcc agactcttcc aaatacgtat atatctcgcg gacgtggtca tcaaaggtgc
     1621 gaatggcttt tttgtaaagt tctacatccc attctttttc tgactcagcg ctatcttcat
     1681 tttcttctac gtgtttgtca tccgtcgaaa ataacggacc atgtgtattc atgtaatgag
     1741 agaagacgaa gaccgggcgg tctgaggttt ccagcaagtt gatgatctgt tgtgtgcgtt
     1801 cttcatcggt catacgggca ttggggttgt tgacttgcag gaagggattt tccatttcct
     1861 tgatgaagaa aatatgaaac aaacggtcgc tggcgcgttc tgtcacagtt tgcagaaaga
     1921 aggcagaggg cgagtttcct aataacggtt gaatggcatc caagatgggg ttatcaacag
     1981 agacgttgtt tactaattca aagccatcag ccaagtttag ctttctcgca tcgacatagt
     2041 aagtggtgcc gagttccacg gttttgtagc ccatatgctt tagaatgccg ggcaaatgct
     2101 cgaaggaatc ttctttggag aggatatcgg gatagcgaaa gacctgaact tctgcaggca
     2161 ttttcccggt caatgcagag gtggtggagc cggtggtgct ggaaacgtta gggaaggcat
     2221 tttcagctac gagggaggtt tgggtcagtt cggcgatgaa gggtgtggta ttgccctcaa
     2281 acccgtatgc ggatagatag ctggcactca aaccatcact gcccaggatt aggatattgg
     2341 ggtgttctgc cgaggcttct gtcgcttcgg gagtaaagtc gctcatgtag ggagagcgcg
     2401 aattgtatat ggagagaatg accactgttg aaacgccgag caaccccaaa gaaaggaaag
     2461 aaacaccttt ccaaagcttt tgtctctcca caaagcggaa gacccaataa aagagcaaca
     2521 caaaggcaac tgtatacaac aagcgccatg ctccagatgt ggagacaatc ccaaatttaa
     2581 aaacggtata ggtgaagtta tcgatcatca ccaaagcagt gacagatacc atcaaggcgg
     2641 ctggaacata cccgaaaaac ttccacttaa gagcaggcac agtaaaaaca accagcaacg
     2701 ccaataaaag cagaacgatc acaccgccgg tcgaaaacaa gacctcgatc ttgtcgagaa
     2761 gggaaagcgt ggatagagac gaagactgcg taacaaaaaa cagccattcc ataagggcgt
     2821 gaaagtatgc ggcgaggacg gtcagattga aaaatagaga taagggattt gtggattttg
     2881 ttttcataaa gtcggcatat tttacacccc tgtattaaaa aaaggcttta ggaactatga
     2941 agaatggatc tagccagaat tagtaccaac gtgctcgatt tcatttctat catttgtatt
     3001 tcaactttga acccagcgct cacgaacgga ctttttagcc tttctttcag ggcgtcgtct
     3061 aaggcttctg ggctttcgcc agtgacctta tacaaccatt tcagg
//
LOCUS       DAOREF010000315         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20774_length_3098_cov_3.628327, whole genome shotgun sequence.
ACCESSION   DAOREF010000315 DAOREF010000000
VERSION     DAOREF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20774_length_3098_cov_3.628327"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKI33_07595"
     CDS             complement(<1..833)
                     /locus_tag="PKI33_07595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNM36908.1"
                     /translation="MNWFNNSRLFELARQGKRLTHIAVVIPLSFIFVVLAQLGIIPLA
                     FIMQAVYGFEDMFQVSLKGFPNTVIGFWMGMQLVFAFGLTYFILWAWLKWFEKRPFWT
                     LGYETKDALSKYGRGFLFGALMFAGAVGILALFGSVTFEQGDPAYQGLPALGGVFLVL
                     IGWIVQGGSEEIVMRGWVLPVIGARYKPWLGLVMSSLIFAILHGLNPNLSVIAMINLA
                     LFGVFAGLYVMREGSLWGISALHTVWNWVQGNFFGLEVSGNGAGGGTLINLVESGADW
                     LT"
     gene            975..>3098
                     /locus_tag="PKI33_07600"
     CDS             975..>3098
                     /locus_tag="PKI33_07600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNM36909.1"
                     /translation="MIKLTIMQPSLEKLRKFFRLEHENKYENTAIIGGLAKMLDYWEG
                     EARADGVAEEVIQAVVSRLRAYEKLNPTGRADSLKGLWKRISETYPEAGQNPKAPPPP
                     QRPNPQPQRPQRSDEDAKPKADAAEQPAAQNQSQPKQHPQQHQQKQQRPNNSPQRFES
                     ATSAKHSQTPAALGAKLTVLNGVGPKNAESLAKLGMQTLGDMLYSFPRRYEDYSLLKP
                     IQDLMYNDVVTVLGTIQSVHNRPIRGGKQSIIEVIISDGTGSLRVSFFNQPWLANRFK
                     TGDAISVSGKIDQYLGRIVMNSPDWENVEMESLSTNRIVPIYSLTERITQKWLRNQMK
                     QVVTFWAPAVVDALPESIKHDAQLVSLGEALTQVHFPDTQDRLKKARERLAFDEIFYL
                     QMGVLRQKRDWQVVEGKRFTASDEWLGARLAALPFTLTSAQQEALNDIRGDLDSGKPM
                     NRLIQGDVGSGKTVIAAMGASIVVSTGAQAAIMAPTSILAEQHYRNFSNLLTGENGFL
                     QKEEVRLLVGNTSEAEKEAIRQGLADGSIKIVIGTHAVIEPDVTFKELQFIVIDEQHR
                     FGVEQRAELRSKGTNPHLLVMTATPIPRSLALTVFGDLDISVMNVMPVGRQPIATYVL
                     RPQERERAYTMIRSQVKNGNQAFIVYPLIEESEKINVRAAVDDFETLSTQVFPDLKLG
                     LLHGRMKPSEKDEVMLKFRDKEFDIL"
BASE COUNT          853 a          742 c          771 g          732 t
ORIGIN      
        1 gtgagccaat ctgcaccgct ttctacgagg ttgatcagag tgccgccgcc tgcgccgttt
       61 ccgctcactt ccaagccgaa gaagttccct tgtacccaat tccaaaccgt gtgcaaggcg
      121 ctaattcccc aaagtgagcc ttctcgcata acatacaaac ctgcgaatac cccgaacaat
      181 gccaggttga tcatggcaat cacgcttaag ttggggttaa gtccgtgcag aatggcgaag
      241 attagcgagg acatgaccag ccctaaccaa ggtttgtagc gagcaccaat tactggcaac
      301 acccatccac gcataactat ttcttcagaa ccgccttgaa cgatccaacc gatcaacaca
      361 agaaagacgc cacctaaggc gggcagccct tggtaagctg ggtcgccttg ttcaaaggtt
      421 acactcccaa acaaggcaag gattcctacc gcaccagcaa acatcaatgc gccgaataaa
      481 aatccgcgcc cgtatttgga cagggcatct ttggtttcat acccaagggt ccagaatggg
      541 cgcttttcaa accacttgag ccatgcccag aggatgaaat aggtcagacc gaaggcaaag
      601 acgagctgca tccccatcca aaaaccaatc actgtatttg gaaagccctt taacgatact
      661 tggaacatat cttcaaagcc ataaaccgcc tgcataataa atgcgagcgg aataatacct
      721 agctgtgcca gcaccacgaa aatgaaggaa agcggaatga ccactgcaat gtgagtgagg
      781 cgctttcctt ggcgcgccag ttcaaaaagc ctgctgttgt tgaaccaatt catgattttc
      841 tcctaattta gtattaataa tttgataata gtgtcaattt gttattaagt caagcccttt
      901 cagtaggttt tttgggggat aagtatcaaa ttcaaagcta ttcgagggat atcgagaatc
      961 ggttcatgcc tgccatgata aaattgacta ttatgcaacc gtctttggaa aaactacgca
     1021 aattttttcg actcgaacac gaaaacaaat acgaaaatac cgccatcatc ggcggacttg
     1081 ccaaaatgtt ggattattgg gaaggggaag cccgcgccga tggcgtggcg gaagaagtta
     1141 ttcaagctgt tgtttcccgt ttacgcgctt atgaaaagtt gaatcccacc gggcgggcgg
     1201 attcccttaa gggcttgtgg aagcgcatta gcgaaactta cccagaagca gggcaaaacc
     1261 caaaagcgcc accaccgccg cagagaccga atccacagcc tcaacgtccg cagcgaagcg
     1321 atgaggatgc gaagccaaaa gcggatgccg ctgaacaacc tgcggcgcag aatcagagtc
     1381 agccgaagca gcatcctcaa caacaccaac aaaaacagca gagaccaaat aattctccgc
     1441 aacgttttga atctgcgacc agtgcgaaac atagccaaac acccgctgcg ttgggtgcca
     1501 agcttacggt cttgaatggg gtgggaccga agaatgctga gtcgttggca aagcttggaa
     1561 tgcaaacgct gggggatatg ttgtattcct tcccgcgtcg gtatgaagat tattcactgc
     1621 tgaaaccgat tcaagatttg atgtacaacg atgtggtgac tgtgttaggc accattcaaa
     1681 gtgtacataa ccgtccgatt cgcggtggaa agcaaagcat tatcgaagtc atcatttctg
     1741 atggcacagg ctcattgcga gtctcgttct tcaaccaacc ttggttggcg aatcgtttca
     1801 agacgggcga tgcgatttct gtttctggaa agatcgatca atatcttggt cgcattgtaa
     1861 tgaacagccc cgattgggaa aatgttgaga tggaaagtct cagtaccaat cgtattgtgc
     1921 cgatctattc gctcactgaa cgcatcactc aaaaatggtt gcgtaatcag atgaaacaag
     1981 ttgttacgtt ttgggcgcct gctgtggtgg atgcgttacc agaaagcatc aaacatgatg
     2041 cgcaattggt ttcactgggt gaagcactta cacaagttca ttttcctgat acgcaagacc
     2101 ggcttaagaa agcgcgggaa cgccttgcct ttgacgaaat tttttatttg caaatgggcg
     2161 tgttgcgaca aaagcgtgac tggcaagtgg tggaaggcaa gcgcttcacc gcctcagacg
     2221 agtggctggg tgcgcgttta gcggctttac ctttcactct cacctctgcc cagcaagaag
     2281 cactcaatga tattcgcggt gatttagact cgggtaaacc gatgaatcga ctcattcaag
     2341 gggatgtggg ttccgggaag accgtcattg cagcaatggg tgcgagcatc gtggtttcta
     2401 caggagccca agctgccatt atggcgccga cttctatttt ggcagaacag cattatcgta
     2461 acttctcgaa cttactaaca ggcgaaaatg gcttcttaca aaaagaagag gttcgcctct
     2521 tggtggggaa tacttctgaa gcggagaaag aagcgatccg ccaggggctt gccgatggct
     2581 cgatcaagat tgtgattggc acccatgcgg tcattgaacc ggatgtgacc ttcaaagaat
     2641 tgcagtttat tgtgatcgat gaacaacatc gttttggtgt agaacagcgc gctgagttgc
     2701 gcagcaaagg taccaatccg caccttttgg tgatgacggc tactccaatt ccgcgctcac
     2761 tggcgttgac ggtctttggt gaccttgata tttcagtgat gaatgtgatg ccggtgggta
     2821 ggcagcctat tgcgacctat gttctccgcc cgcaggaacg cgaacgtgca tatacgatga
     2881 ttcgctcgca ggtgaaaaat ggcaatcagg cgttcattgt gtacccactc attgaagaga
     2941 gcgagaagat caacgtccgc gcggcggtgg atgatttcga aacattgtct acacaggtgt
     3001 ttcctgacct caagcttggt ctcttgcacg ggcggatgaa gcccagcgaa aaagatgaag
     3061 tgatgctcaa gttccgcgat aaggaatttg atattctc
//
LOCUS       DAOREF010000316         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20896_length_3088_cov_4.021101, whole genome shotgun sequence.
ACCESSION   DAOREF010000316 DAOREF010000000
VERSION     DAOREF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20896_length_3088_cov_4.021101"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PKI33_07605"
     CDS             <1..827
                     /locus_tag="PKI33_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNM36910.1"
                     /translation="LMKDTLKQIIPQPLWQFARDTYDGFRRIPQLPEAYFHPWRRESI
                     KRLAALKDIHKGKRAFIIGNGPSLKQTDLSKLKNEITFGMNRFYLAFPDLGFTTSYIC
                     VTNDLVIEQFVNDFLSLQVPQFIAWRSHRHYPAGLPTSNLPTFIYTTYTGPRFTTDVR
                     ARVWEGATVTNLALQMAYHMGIEKAILIGVDHNFTSKGDANKTVVSEGDDPNHFMPNY
                     FGKGVKWQLPDLDTSEVGYIMAREAYAKAGRQVVDATIGGKLTVFPKVDYNSLFIK"
     gene            892..>1361
                     /locus_tag="PKI33_07610"
     CDS             892..>1361
                     /locus_tag="PKI33_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNM36911.1"
                     /translation="MFSSNKKIGFAFAVAIFIGILLSSCSNASKKVTVLPVEVRPNII
                     LILADDLDQKLGTTDYMPNLKTYLIDRGTTIENFLITTPMCCPSRINLLRSQYTHNHT
                     VFNNTAPNGGFPKFFETGFEKSTLPVWLQAAGYRTALIGKYMNAYPLADNRTYVP"
     assembly_gap    1362..1461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1462..2055
                     /locus_tag="PKI33_07615"
     CDS             <1462..2055
                     /locus_tag="PKI33_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36912.1"
                     /translation="TSDQGFTLGEHRIIEGKSSFFEEDIVVPFVVRGPNIPEGEKITG
                     ELATIIDIAPTISSWAGVIPPNFVDGKSFAGLLDKNSVKNSHIRNNFLLEMYAFKGEE
                     QGSKFPNIFATLVFPPLSEKENIPKMSGLRTLEYTYLERPDGFIELYNLTQDPYQLQN
                     LASTASIELLEKFSTLLHKMENCKASECMLLDQEVVP"
     gene            2127..>3088
                     /locus_tag="PKI33_07620"
     CDS             2127..>3088
                     /locus_tag="PKI33_07620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM36913.1"
                     /translation="MKSSPLSRTNLWSGLFILLLALGGLLRLLDLTDPPLDFHTSRQL
                     RNSLVAREIYYNTLPSATEEQRQLATSFANAVGKYEPPVIETIVGYSYFLTGGETIAV
                     ARIWETFFWLVAGIALFDLMRRVVNPWAALIAMAYYFVLPFSVEVSRSFQPDPLMTSA
                     FVIGIYFLYRWSELWGTEGEGKETWKWALLAAVFLGFATFVKIVIAFFVGTAAVSLVL
                     FTLGKDFWKSKQVWIMAAIMVIPALLYYVVFNQGRSTEYFFSWTVALMKLITSTDFYT
                     KWLAFLGTLFGLTILFLSIAGAIIAPSRMRWLLVSLWVGYLLYG"
BASE COUNT          783 a          640 c          604 g          961 t
ORIGIN      
        1 acctcatgaa ggatactctc aagcagatca ttcctcagcc tctctggcaa tttgcgcgcg
       61 acacctatga tggctttcgt cgcatccccc aactccctga agcgtatttt catccatggc
      121 ggcgtgagag tattaagcgt cttgcagcac tcaaagatat tcataaaggc aaacgtgcct
      181 tcattatcgg aaatggaccc tctctaaagc aaactgacct tagcaagctc aagaacgaaa
      241 ttacctttgg tatgaatcgt ttttatcttg ctttcccaga ccttggtttt acaacttcat
      301 acatttgcgt tactaacgac cttgtcattg aacaattcgt caatgacttt ctttctttgc
      361 aagtgccgca gttcattgct tggcgttctc atcgtcatta ccctgcgggt ctgccaacct
      421 ctaatttgcc aacctttatc tacaccacct ataccggtcc gcgctttaca accgatgtcc
      481 gtgcgcgggt ttgggaaggc gccacagtta ccaacctagc tttgcaaatg gcatatcata
      541 tgggcattga aaaagcgatc ttgatcggtg tcgatcacaa cttcacatca aaaggtgatg
      601 caaacaaaac agtcgtctct gaaggcgatg acccaaatca cttcatgccc aattattttg
      661 ggaaaggcgt caaatggcag ttgcctgacc ttgatacatc cgaagtcggt tacattatgg
      721 cgcgcgaagc ttatgccaaa gcaggtcgcc aggttgttga tgcaaccatc ggtggtaaat
      781 taaccgtctt cccaaaagtg gactacaatt cactcttcat taagtagtag tttcagtttg
      841 tttggctaga aaatctaaag ggagtttgaa aattatgaaa ggcgttttgt aatgttttct
      901 tctaataaaa aaattggttt tgcttttgct gtagctattt ttatagggat tcttttgtct
      961 agttgtagta atgcaagcaa aaaggtaact gttttaccag ttgaagtccg cccaaatatc
     1021 attcttattc ttgcagatga cttggatcaa aaattaggca caacagacta tatgcctaat
     1081 ctaaagacat atctaattga ccgcggtact acaattgaaa atttcttaat tactacacca
     1141 atgtgttgtc catctcgtat taatttattg cgcagtcaat atacacataa ccatacagta
     1201 ttcaataata cagcgcctaa tggggggttt cctaaatttt ttgaaactgg gtttgaaaaa
     1261 tctactctgc cggtgtggtt acaggctgca gggtatcgta cagcattgat tggcaaatat
     1321 atgaatgcat atccattagc agataatcgt acatatgttc cnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nacttcagat caaggattta ctttaggtga gcatcgtatc
     1501 attgaaggaa aaagttcgtt ctttgaggaa gatattgtgg ttccttttgt tgtgcgtgga
     1561 ccaaatattc ctgagggaga gaagattacc ggcgaacttg ctactattat tgacatcgcc
     1621 cctactattt ctagttgggc tggggttatc cctcccaatt ttgtagatgg taagtcgttt
     1681 gcaggtttat tggataaaaa ttcagttaag aattctcata taagaaataa ttttttgctc
     1741 gaaatgtatg cttttaaggg cgaagaacaa ggctccaaat ttccaaatat atttgcaact
     1801 ttggtttttc cgcctctatc tgaaaaagaa aatattccaa agatgtctgg tttaagaaca
     1861 ttggaatata catatttgga acgtcctgat ggttttatcg aactttacaa tcttactcaa
     1921 gacccatacc aattacaaaa tttagcttct acagcttcga tagaattgct ggaaaaattt
     1981 tctacattac ttcataaaat ggaaaactgt aaagcgtctg aatgtatgtt gcttgatcaa
     2041 gaagttgtgc cttagtaaac taagagtaca gaaaacatat ttctattagt tagataaaat
     2101 atacaagtga atttggatat tttttcatga aaagttcgcc tctttcccgt accaatctct
     2161 ggtctggtct cttcatcctt ctcctcgctc taggcggact cttacgcctt ctcgacctta
     2221 ctgaccctcc actagacttt catacctcac gtcagctgcg caattcacta gttgcgcgtg
     2281 agatttatta caacaccttg ccatctgcta ccgaagaaca acgtcaactt gcaacatcat
     2341 ttgcaaatgc agttggtaaa tatgagcctc cggttatcga aacgattgtt ggctattctt
     2401 actttttgac tggcggcgag accattgccg tagctcgtat ttgggaaact ttcttttggc
     2461 ttgtagctgg cattgccctc tttgacctga tgcgacgtgt ggtcaacccg tgggcagcat
     2521 tgatcgctat ggcatattac tttgttttgc ccttttctgt agaagtcagc cgttctttcc
     2581 aaccagaccc attaatgacc tctgcttttg tgatcggcat ttacttcctt taccggtgga
     2641 gtgaactttg gggaacagaa ggcgagggaa aagagacttg gaaatgggca cttcttgccg
     2701 ctgttttcct cggctttgcc accttcgtaa agattgtcat tgcctttttt gtgggtacgg
     2761 ctgccgtctc tttggtgctt ttcacccttg gcaaggattt ctggaagtcg aagcaagttt
     2821 ggataatggc tgccatcatg gtgatcccgg cattgctgta ttacgtggtc tttaatcagg
     2881 gacgttccac tgaatatttc ttttcatgga cggtagcatt gatgaagctc attaccagta
     2941 cagattttta caccaaatgg ctagcattct taggtacgct cttcggcttg actattcttt
     3001 tcctcagcat tgctggcgca attattgccc catcacggat gcgctggttg ctcgtcagct
     3061 tatgggttgg ttatttgctt tacggctt
//
LOCUS       DAOREF010000317         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20912_length_3087_cov_3.062335, whole genome shotgun sequence.
ACCESSION   DAOREF010000317 DAOREF010000000
VERSION     DAOREF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20912_length_3087_cov_3.062335"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PKI33_07625"
     CDS             complement(<1..914)
                     /locus_tag="PKI33_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNM36914.1"
                     /translation="MSTDLDPAELKQKFYSLRTFEDLASLLDVPKNKLYYYTYKSKKD
                     HYKSFSIPKKTWGVRKIYAPSTPLKIIQHKLNQVLQAVYLPKASVHGFVQKKSILTNA
                     KRHLENGKKRYILNIDLEDFFPSITEKRVARLLMAVPYNLPFQVASAIAQLCCLNNAL
                     PQGAPTSPILSNMICAKMDTQIRILAQQLKCTYTRYADDISISTTLPKFPPALATIDN
                     EINTFVVGEHLQSIILNNGFKINNKKTRLLTKNDRQEVTGLTVNKFPNVRRNYVRQIR
                     AMLYAWEEFGLERAQKEYQEKYFPQWKGA"
     gene            complement(1221..2870)
                     /locus_tag="PKI33_07630"
     CDS             complement(1221..2870)
                     /locus_tag="PKI33_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNM36915.1"
                     /translation="MASYDAIIIGSGAGGLTAAVALAQAGKKVLVLEQHDRPGGWTHS
                     FTLEGYRFSPGVHYIGDLQEGGGLRRIYDGVGVSQDLSFVELNPNGYDHIVVGDKQIN
                     FPKGKENLIAELKRNFPQEAQGIDGYFNDLMSMINGLKNVGNLSSPLDAARGAGNVLK
                     WMRATGADLINAHINDPVLRGVLAGQSGDHGLPPSQVSAFMHAGITHHYLEGGYYPLG
                     GAFTIPRAFVRALKRAGGEIRLKSRVKRIMLGGRKVIGVEMESGEEILADVVISNADP
                     EVTFGKLIGRDKLSPRLQKKIDSVRYSTSCLSLFFATDMDLRGAGLDSGNMWYYDHPD
                     VDKIYTDGLTDAVLKDEGMPGMFLTVTTLKDPSKMHSGHHTCESFAFVGYDAFEKWAQ
                     TKYGSRPADYEAMKEDLAWRMVRGLEKRIPGLSKHIVYYSLGTPLTNEHYLNATRGNL
                     YGIDKLPKQIGPLGFTPRTEFDGLYLCGASTMSHGVAGVTASGIDAAKAILNVRSREI
                     LTQKGNGPLFLQAEDVSSWPEHLKRKMEKGEAAREEEEREI"
BASE COUNT          791 a          770 c          633 g          893 t
ORIGIN      
        1 gcacccttcc attgaggaaa atacttctcc tgatattctt tttgagctct ttctaaacca
       61 aattcttccc aagcatacaa catagccctt atttgtctaa cgtagtttct tcgaacattt
      121 ggaaatttat taacagttaa ccctgtaact tcttgcctgt cattttttgt caaaagtcta
      181 gtctttttgt tattaatctt aaatccatta ttaagaataa tggactgcaa atgttcccca
      241 acaacaaaag tattaatttc attatcaatt gtagcaagtg caggtggaaa ttttggtagc
      301 gtggttgaaa tacttatgtc atctgcatat ctagtgtaag tacattttaa ttgttgagca
      361 agtatcctta tttgcgtgtc catttttgca caaatcatgt ttgacaaaat tggagatgtg
      421 ggtgccccct gtggtaatgc attatttaga cagcaaagtt gcgcaatagc tgatgctact
      481 tgaaatggca aattgtatgg aacagccatt aataatctag ctactctttt ctccgttatt
      541 gaagggaaaa aatcctctaa atctatatta agaatatatc ttttcttgcc attctcaaga
      601 tgcctttttg cgttagttaa tatgcttttt ttctggacaa aaccatgtac agaggctttt
      661 ggtaaataaa cagcctgtaa aacttgatta agcttgtgtt gaattatctt tagcggggtt
      721 gatggcgcat aaatttttct aactccccaa gtttttttag gaatactgaa acttttgtaa
      781 tgatcttttt ttgatttata agtatagtaa taaagcttgt ttttaggtac atccaaaaga
      841 ctagccaaat cttcaaatgt tcgaagagaa taaaatttct gtttaagctc ggcagggtct
      901 aagtctgtac tcatatctta ttagataaga agaggtactt gaaggaaaat tcaaactgct
      961 gcggctgaca gcgcgcatac gatcttgcgt agctaagggt aacaacaaaa atattcggcg
     1021 caggatcaac accgaaaatc aattattcct tcaagtaccc tttgaacaaa attatagcat
     1081 atggttttaa tatcctttaa ttcactttac caatcgcatg acgatttaat gctttaaaat
     1141 accaaggtga cagtcacttt tgaagtgact gtcacctgat tccttttatg ccacaaactc
     1201 aaaatggcta tgcgctattt ctaaatctcg cgttcttcct cttcgcgtgc cgcctcgccc
     1261 ttctccatct tgcgcttcaa atgctcaggc catgaagata catcctccgc ctgcaagaac
     1321 agcggaccat tgcccttctg ggtcaaaatc tcacgcgagc ggacattcaa gattgccttc
     1381 gctgcgtcga tgccagaagc ggtcacacct gccacgccat gactcatcgt gcttgcacca
     1441 cacagataca agccatcaaa ctcggtgcgt ggcgtgaagc ccaacggacc tatttgtttc
     1501 ggaagtttat caataccata taaatttccg cgcgtggcat tgagataatg ttcattcgtg
     1561 agcggcgtac cgaggctgta atacacaata tgcttgctca aaccgggaat acgtttctcc
     1621 agtccgcgca ccatgcgcca ggcaaggtct tctttcatcg cttcgtaatc cgcggggcgt
     1681 gagccgtatt tcgtctgcgc ccatttctcg aaggcgtcat agcccacaaa agcaaaggac
     1741 tcgcaggtgt gatgtccgct gtgcattttc gacgggtctt tcaacgtggt gaccgtcaga
     1801 aacatgccgg gcataccctc gtctttcaat acagcatcgg tcaagccgtc ggtataaatt
     1861 ttgtccacat cggggtggtc gtaataccac atattgcccg agtcgagtcc agcgccacgt
     1921 aagtccatat cggttgcaaa gaaaaggctc aagcaagaag tggaatatcg gacggaatca
     1981 attttcttct ggagtcgagg cgagagtttg tccctgccga tcaacttgcc gaaggtcacc
     2041 tcggggtctg cgttggagat gacgacatcc gcaagaatct cttcgcccga ctccatttcc
     2101 acgccgatca cttttctacc tccaagcatg atacgcttca cacgtgactt gagcctaatc
     2161 tcacccccag ctcttttcag cgcccgcaca aacgcccgcg gaatggtgaa cgccccaccc
     2221 agcggataat atccgccttc aagataatga tgcgtgatgc ccgcatgcat gaacgcgctg
     2281 acctgcgaag gcggcaagcc atggtcgcca gattgacccg ctaacacacc gcgcaagacc
     2341 gggtcgttga tgtgcgcatt gatgaggtcc gcaccagtag ctcgcatcca cttgaggacg
     2401 ttacccgctc ctctcgctgc atctaatggg ctcgataaat taccaacatt tttcaagccg
     2461 ttgatcatac tcatcaggtc gttgaaatac ccgtcaatgc cttgcgcctc ctgcgggaaa
     2521 ttccgcttga gttcggcgat caaattctct ttgcccttcg ggaagttgat ctgcttatcg
     2581 ccaacgacga tatggtcata tccgttcggg ttgagttcga caaagctcaa atcttgcgac
     2641 acgcccacgc catcataaat tcggcgtaac ccgccgccct cttgtaagtc gccgatgtaa
     2701 tgcacgcccg ggctaaagcg atagccttcg agcgtaaaag aatgcgtcca cccgccgggt
     2761 ctgtcatgct gttcgagaac gagtaccttt ttgcccgctt gcgccaatgc aacggctgct
     2821 gtgagtccac ctgcgcctga accgatgatg attgcgtcgt atgatgccat gaattcctcc
     2881 gtggattcgt agggcgaaag ttaatgccct gtcttagttt tcgctctttg tcactaccag
     2941 ccctactcga tactcttctg ccttgatctc ccctatttta tcttgcggaa aaggcaaaat
     3001 gctctttacg atcacttcat atccattgtg ctcaatacga gttgtcattc cctcaaattc
     3061 catctcaggc accttgccat cgttatc
//
LOCUS       DAOREF010000318         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20945_length_3084_cov_4.484979, whole genome shotgun sequence.
ACCESSION   DAOREF010000318 DAOREF010000000
VERSION     DAOREF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20945_length_3084_cov_4.484979"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /gene="mce"
                     /locus_tag="PKI33_07635"
     CDS             complement(<1..364)
                     /gene="mce"
                     /locus_tag="PKI33_07635"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNM36916.1"
                     /translation="MPQIKAINHVAVVVEDMEKSLAFWRDALGIKLHELRDVPAEKSQ
                     VAFLPLAGAEVELVMPTTDDSGIAKYLAKRGQGMHHLCLEVDDIEGMMAQLKAKNIRL
                     INEEPRTAADGKKYAFIHP"
     gene            complement(396..995)
                     /locus_tag="PKI33_07640"
     CDS             complement(396..995)
                     /locus_tag="PKI33_07640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNM36917.1"
                     /translation="MPLRYLALGDSYTIGESVEEAERFPNQLASLLANEGVTVDVKII
                     ARTGWTTDELWRGIQAEVVEPPYDMVSLLIGVNNQYRGRGLEEYREGFIFLLNKAIEY
                     AGGDVKRVVVFSIPDWGVTPFAYGRDTTQIANEIDAFNKVNLEESTKAGAHYVDVTPS
                     SRLALNDKTLIASDGLHPSGKMYAEWAQSALPIALNILK"
     gene            complement(1114..1524)
                     /locus_tag="PKI33_07645"
     CDS             complement(1114..1524)
                     /locus_tag="PKI33_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HNM36918.1"
                     /translation="MKAKLTVFFEDPYWVGVFEREDEKEYQVARAIFGCEPTDAELYE
                     FLQANYRNLEFSRSFQGEIQLERKVNFKRKQREVRKQANENGIGTKAQQAIKQVMEES
                     KKGRKRDVRQQRREEEKLKFVLKQEKKKEKQKGH"
     gene            complement(1713..2660)
                     /locus_tag="PKI33_07650"
     CDS             complement(1713..2660)
                     /locus_tag="PKI33_07650"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNM36919.1"
                     /translation="MQAHANWEHQLLSLAGHIPHPSTRPFFSYWAGDATLRAAYKQAE
                     VITSAHSKSFYFASNLLPEEKRSAVRALYAFCRTVDDIVDEVEIKKDRDFELGYWRNV
                     VETASTSTDDLVASAWADTLVRYHIPRHYALQLIDGVARDLSQSRYQTFDDLATYCYG
                     VASTVGLMSMYIVGFKSNEAVPYAIKLGVALQMTNILRDVGEDYRNGRLYLPREELVT
                     YGIREEDIAEGRVTDAWRQFMKFQIERTRHLYEASWQGVKLLEREGQLAIGAASIFYQ
                     GILDDIEKHDYDVFSRRASLSAMGKVAKLPALWMKIRSL"
     gene            2806..>3084
                     /locus_tag="PKI33_07655"
     CDS             2806..>3084
                     /locus_tag="PKI33_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM36920.1"
                     /translation="MKPTALVIGAGIGGIATAARLAKNGYDVTVLEKESTPGGRCNQI
                     VRDGHRFDIGPTLFLMPEIWEETFASLGEKMSDHLELKRIDPTYKVHFD"
BASE COUNT          787 a          756 c          728 g          813 t
ORIGIN      
        1 cagggtggat gaaagcatac ttcttcccat ccgctgcggt gcgcggttct tcattgatga
       61 gccgaatgtt tttcgctttc aattgtgcca tcatgccctc aatatcatcc acttcaagac
      121 ataaatgatg catcccttgc ccacgtttgg caagatactt ggcaatgcca gagtcgtcgg
      181 tggtgggcat caccaattcc acctcagcgc cagccaaagg cagaaacgcc acttgagatt
      241 tctcggcggg cacgtctctt aattcatgta gtttaattcc cagggcatcg cgccaaaacg
      301 caagcgactt ttccatatcc tcgacaacaa cggcaacatg attgatagct ttgatctgag
      361 gcattgattc tccgtttttg atttatgaaa agattctact tcaagatatt caacgcaatc
      421 ggtaacgcag attgtgccca ctcagcatac attttgccag agggatgcaa tccatcggac
      481 gcaatcagag ttttatcgtt gagcgctaag cgggaactag gggttacatc tacataatga
      541 gcacctgctt tggtggactc ttcgaggtta actttattaa aagcgtcgat ttcatttgcg
      601 atctgggtgg tgtctctgcc atatgcgaac ggagtgacac cccaatctgg aatagagaag
      661 acaacgaccc gttttacatc tccgccagca tattcgatgg ctttatttag caaaaatatg
      721 aacccttcgc gatattcttc aagccctctg ccacgatact gattatttac gccaatcagt
      781 aaagagacca tatcataggg cggctccacc acttcggctt gaatgccgcg ccaaagctca
      841 tctgtcgtcc agccggtgcg agcgatgatt ttcacatcaa ccgtcacacc ttcatttgca
      901 agcaatgatg caagttgatt tgggaagcgc tctgcttctt ccacactttc accgatggta
      961 taagagtcac ctaaagccaa gtatcgcagg ggcatgacag tctccggtgt aggggttgat
     1021 ttggtcgagc aactaataag aagtagacaa aacaagatcg cgaatttctt cacgaaggca
     1081 agtatatcaa aagacgctgg gcattagatg tacctaatgc cccttttgtt tttctttctt
     1141 cttttcttgc ttaagtacga actttagctt ttcctcttca cggcgttgtt ggcgcacatc
     1201 ccgctttcgt cctttttttg actcttccat cacttgcttg atcgcctgtt gagctttggt
     1261 gccgatccca ttttcgttcg cttgcttgcg gacctcacgc tgtttgcgtt taaagtttac
     1321 tttcctttca agttgaattt ctccttgaaa ggagcgacta aattcaagat tgcgatagtt
     1381 agcttgaaga aactcgtata actctgcatc ggttggctcg cagccaaaaa tagcccgcgc
     1441 tacttgatat tctttttcat cttctctctc aaagactccc acccaataag gatcctcaaa
     1501 aaagacggta agttttgctt tcatacttcg ctccaaatcc atgatcaata gaaacaaaaa
     1561 ggactggcgg aatcttcacg ccagatgtat ggtcaattca attgtgggaa atggagtttg
     1621 ttctagataa gcaaagtgta tcattaaata ggcaagggca tgactgcccg gtcacgccct
     1681 tgcacacaca agaggagaat gatgctagcg aattataggg agcggatttt catccacaaa
     1741 gcggggagtt tggcaacttt acccattgcg ctgagagaag cacggcggga gaagacatcg
     1801 taatcatgct tttcgatatc gtccaaaata ccctgataaa aaatcgaagc ggcgccaatc
     1861 gcaagttgac cttcgcgctc aagcaatttc acaccttgcc aagaggcttc gtaaagatgg
     1921 cgagtgcgtt cgatctggaa tttcatgaac tgacgccacg catcggtgac gcgaccttcg
     1981 gcaatatctt cttcacgaat gccataggtg acaagttctt cgcgtggcag gtaaagacga
     2041 ccattgcggt aatcctcccc cacatcgcgc aggatgttgg tcatttggag agcgaccccc
     2101 agcttgatgg cgtatggcac ggcttcgttg ctcttgaacc caacgatata catgctcatc
     2161 aacccaacag tggaggcaac gccatagcaa taggttgcga ggtcgtcgaa ggtttggtaa
     2221 cggctctggg aaaggtctcg ggcaacgcca tcgatcaact gcaaggcgta gtgacgcggg
     2281 atgtggtatc ggaccaaggt atctgcccaa gcggaggcga ccaagtcatc ggtcgaggtt
     2341 gaggcggttt ctacaacatt gcgccagtag cccagttcaa agtcacgatc tttcttgatt
     2401 tccacttcgt ccacaatgtc atccacggtg cggcaaaaag catataaggc gcggacggcg
     2461 gaacgttttt cttcgggcaa caaatttgag gcaaagtaaa aagatttgct atgcgcagaa
     2521 gtgatgacct ctgcttgttt gtaggctgcg cgcaaggttg catcgcctgc ccaataggag
     2581 aaaaacgggc gggtagatgg gtgcgggatg tgacccgcga gggacaggag ttgatgctcc
     2641 caatttgcat gtgcttgcat aagtctctct ccttgttggt taggttgttc gtattttact
     2701 gattatgtca tatttgtcaa ggttttgcaa atgtatttgc aaaaccttga caaatcatag
     2761 acgacgcgta caatacactc actgacctcg aaaaggagaa agtctatgaa acctactgct
     2821 cttgtaatcg gggctgggat tggcgggatt gccaccgccg cccgccttgc taaaaatgga
     2881 tatgacgtga ccgtgctgga aaaggaatcc acccccggcg gacgctgcaa tcagatcgtc
     2941 cgtgacgggc accgcttcga tattggtccg accctgtttc tcatgcccga gatttgggag
     3001 gaaacctttg cctctctcgg cgaaaagatg agcgaccacc tcgaattgaa acgcatcgac
     3061 ccgacatata aagtccactt cgac
//
LOCUS       DAOREF010000319         3082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_20979_length_3082_cov_3.041956, whole genome shotgun sequence.
ACCESSION   DAOREF010000319 DAOREF010000000
VERSION     DAOREF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20979_length_3082_cov_3.041956"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..976
                     /locus_tag="PKI33_07660"
     CDS             359..976
                     /locus_tag="PKI33_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNM36921.1"
                     /translation="MSVRNAILGLLAQKSRHGYELHSAFSTVMGGATWDVKPAQIYTT
                     LERLQESGLVQTKSDLGEGREPDRRIYAITRDGHEALKSWFNESVPTEHQRDEFYVKL
                     MIGLISGEADPARIIQTQRSRLFQEMHDATTQRDKYDPRLEMAKILLSDKAIMHLEAD
                     LRWLDMIEMRLETIKDQPMPEPEIRKRGRPKKIDAGSTYPPPDEK"
     gene            1035..1778
                     /locus_tag="PKI33_07665"
     CDS             1035..1778
                     /locus_tag="PKI33_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM36922.1"
                     /translation="MSNIQIEKLTKTYGSGITQVTALDHVNLTIREGEFVAIMGPSGC
                     GKSTLLHLLGGLDKPSEGSVLIGGHNLTDLNDDKLTELRRRKIGFVFQFFNLIPVLNA
                     TENAALPVTLDGLKLADAQKKAVEWLTRFGLADRLNNRPDQLSGGQQQRVAIARALVA
                     DPELILADEPTGNLDTRSSDEIASLLRDVSKKYNRTVVMVTHDPRIAAYADRIIFLKD
                     GKVVDETLLERKNGKSADYVAGKVKELGD"
     gene            1780..>3082
                     /locus_tag="PKI33_07670"
     CDS             1780..>3082
                     /locus_tag="PKI33_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM36923.1"
                     /translation="MKFQITLAARYLSGRKLRTFLTTLAVVFGVLVIFGMNIIMPTML
                     SALQVNVQSAEGKVDYTVTHISGEPFSETVAENLAAVDGVRVYAASLNRTINIPADFF
                     DKDTSKLDTITAISLVGINPEAERSLRAFSLVDGRYLQADDSNSALVSRTFADSLHLE
                     VGDTFLLPSADGTTELTVVGILPAQLTSGNETIWVNLPVAQTIMNALGKVNTISINTE
                     TTAMEERRAEVQTNIETTLGDQYHVGSLLSGTEMFASLELAQTMFNVFGVLALFMGGF
                     IIFNTFRTIIAERRRDIGLLRALGANRRTVIGIIIAEGLLQGILGTGLGLLLGYLFGW
                     GAIRVAEPFMSQFVNLSLGKPVISPILLFGSIFLGVGVTVIAGLIPAFAASNVTPLEA
                     LRPSAAEMHHGRQTGFGFVGGAFIILLTVLAILTGNPTLIIP"
BASE COUNT          741 a          779 c          793 g          769 t
ORIGIN      
        1 gcgcatggca tggcagcagt cgctttttgg cgggcggggt tgatggtgaa gatggtgggg
       61 ggaaggctta tgatgtggaa taagggaaat atagtgagta atgagtaaag aggaataagt
      121 aacgagttga tagccaaatc cgtgaggtga aagccttgcg gattttttga ttcgcgtaga
      181 tttgcgcaga ttagcggaca agctttcaga ggagaatcgc ttccaggaag ccgtatcaag
      241 ataaggtcaa gaaagaggcg ggagtgattt tcctcctctt gacaaaaaat agtaactcgt
      301 ataatatttt cccgttttgt aattagtaac tcgtatacta tttttaagtg aggcactctt
      361 gtccgttcga aatgccattt taggtctgct tgcgcaaaaa tcccggcatg ggtatgaact
      421 ccattctgcc ttttccacgg taatgggtgg ggctacttgg gatgtgaagc ctgcccagat
      481 ctacaccact cttgaacgtt tgcaagaatc tggcttggta caaaccaagt cggatctggg
      541 agaagggcgg gagccagatc ggcgcattta tgccatcact cgcgatggac atgaagccct
      601 gaaatcgtgg ttcaacgaaa gtgtgccgac cgaacaccag cgtgacgagt tttacgtcaa
      661 actgatgatc gggttgatct ctggtgaagc tgacccagcg cgcatcatcc aaacccagcg
      721 ttcacggctt tttcaggaaa tgcacgacgc cacgacgcag cgcgataaat atgatccgcg
      781 cttggagatg gcaaagatcc ttttgagtga taaagccatt atgcacctcg aagcggactt
      841 gcgctggctc gatatgatcg aaatgcgttt ggaaaccatc aaagatcagc ccatgcctga
      901 gcctgaaatt cgcaagcgcg gcagacctaa aaagattgat gcaggttcta cgtatccgcc
      961 gcccgatgag aaataagtca tagtagttgc aagttttatt cgttcgcatt gcttttataa
     1021 agagaaagaa aaaaatgtcc aatattcaga tcgaaaaact taccaaaact tatggctcag
     1081 gcatcacgca agtgaccgcg cttgaccatg tcaacctcac cattcgcgaa ggcgaatttg
     1141 tcgccattat gggacccagc ggctgcggca aatcgactct gttgcatttg ctcggcggct
     1201 tggataagcc cagtgaaggc agtgtgttga tcggcgggca caacctgacc gacctcaacg
     1261 atgacaaact caccgaactg cgtcgccgca agatcggctt tgtcttccaa tttttcaacc
     1321 taattccagt tttgaacgct acagagaacg ctgcgttgcc tgtcactttg gatgggctca
     1381 agcttgctga tgcgcaaaag aaagctgtgg aatggctgac tcgtttcggc ttggcagatc
     1441 gtttgaacaa ccgtcccgac caactctctg gcggacaaca acaacgcgtc gcgattgccc
     1501 gtgccctggt cgctgacccg gaactgatcc tcgccgatga gcccaccggc aacctcgaca
     1561 ctcgctccag cgatgagatc gccagcctct tgcgcgacgt ctcgaaaaaa tataaccgca
     1621 ccgtggtcat ggtgactcat gatccgcgta tcgctgctta tgccgaccgc atcatcttcc
     1681 tcaaggatgg caaggtggtg gatgaaacct tgctcgaacg aaaaaacgga aagtctgctg
     1741 attatgtggc aggcaaggtg aaagaattgg gtgattgaaa tgaagttcca gatcactctc
     1801 gccgcccgct acttgagcgg acgtaaatta cgaaccttcc tcaccaccct tgctgtggtc
     1861 tttggggtgc tggtcatctt cggcatgaac atcatcatgc cgaccatgct cagtgcctta
     1921 caggtcaatg tgcaaagtgc cgaagggaag gtggattaca ccgtcaccca catctcgggt
     1981 gaaccctttt ctgaaactgt ggcagagaac cttgcagctg tggatggtgt gcgtgtttat
     2041 gctgcttcat tgaaccgtac catcaacatc cctgccgatt ttttcgacaa agatacgagc
     2101 aagctcgata ccataacagc gatcagcctt gtgggcatca accccgaagc cgaacgctcc
     2161 ctgcgagctt tttctttggt ggacggtcgc tacttgcaag ctgatgacag caattctgct
     2221 ttggtctcgc gcacatttgc tgactctctt cacttggaag taggggatac cttcctgctc
     2281 ccgagcgccg atggcacgac agagttgact gtggttggca ttctgcctgc acagctaacc
     2341 tctggcaatg agacgatttg ggtcaaccta cctgtagcgc aaacgatcat gaatgctttg
     2401 ggcaaggtca acaccatcag catcaatacc gaaacaactg ccatggaaga acgccgtgct
     2461 gaagtgcaaa caaatattga aacaacattg ggagatcagt accacgttgg gtctttactc
     2521 tctggtacag agatgttcgc ttcacttgaa ctcgctcaga ctatgtttaa tgtctttggc
     2581 gtgttggctc tctttatggg cggcttcatc atcttcaaca ccttccgcac catcatcgcc
     2641 gaacgccgcc gcgacattgg tttgttgcgt gcattgggtg ccaatcgccg tacagtgatc
     2701 ggcatcatca ttgccgaagg acttttgcaa ggcatccttg gcacagggct gggattgctc
     2761 ttgggctatc tctttggctg gggcgccatt cgtgttgcag aacccttcat gagtcagttc
     2821 gtcaatctga gtctaggcaa gccggtcatt tcgccgatcc ttctttttgg ttcgatcttc
     2881 ctcggtgtcg gtgtgaccgt cattgccggg ttgatccctg cctttgctgc cagcaatgtc
     2941 actccgctcg aagcgttaag accgtcagcg gcagagatgc atcatgggcg gcaaacaggc
     3001 ttcggctttg tcggaggcgc gttcatcatc ttgctcacgg tgcttgccat tctcacaggc
     3061 aaccccacac tcatcatccc gg
//
LOCUS       DAOREF010000320         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21076_length_3074_cov_4.100033, whole genome shotgun sequence.
ACCESSION   DAOREF010000320 DAOREF010000000
VERSION     DAOREF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21076_length_3074_cov_4.100033"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PKI33_07675"
     CDS             <1..1095
                     /locus_tag="PKI33_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNM36924.1"
                     /translation="PEEADAFIIAVPTPFKHNETGNYNGMTYKLADMEAVTSAAESIV
                     PYLRKGNVVVLESTSPPRTTFDLVAPILEKSKLKAGVDFHLCYSPERVLPGQILRELI
                     ENSRVIGGVTPESAKAGADLYSTFVKGQIIQTDATTAEMVKLMENTYRDVNIAIANEF
                     SRLADKFGVDVWEAISIANLHPRVKILSPGPGVGGHCISVDPWFFVEAAPNLTPLIYN
                     SRQVNDAQPAFVVDVVKRALGSLKGKKIAALGLAYKPDVDDLRESPAVEAVHFLQKEG
                     AEVLACEPFKPDAKLPGVTIAPTFEDAVRDADAIILLVKHTEFSKLKPEQVAQQTKAR
                     LLIDTVQGWDAEQWKHAGFRHVKLGSGKPA"
     gene            complement(1096..1251)
                     /locus_tag="PKI33_07680"
     CDS             complement(1096..1251)
                     /locus_tag="PKI33_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36925.1"
                     /translation="MFKLIKFTKRSIILALYFSLILGLASCAKATCGMQAVAGGSQTC
                     IEVLNAE"
     gene            1300..2412
                     /gene="wecB"
                     /locus_tag="PKI33_07685"
     CDS             1300..2412
                     /gene="wecB"
                     /locus_tag="PKI33_07685"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNM36926.1"
                     /translation="MRILTVFGTRPEAVKMAPIVKLLASTPGVEARVCVTAQHRQMLD
                     QVLNLFQIEPHYDLNLMRDGQSLAQLSADIFTHLDPVLEDFKPDWVLGQGDTTTVAIT
                     SMLCFYRQIRFGHVEAGLRTHDKWQPFPEEVNRRIVSVIADLHFAPTNWSKNNLLREG
                     VDEKTVRVTGNTVIDALLFVSKQAEPAEITELVQSKLSHKKLILVTAHRRENFGAPME
                     NICKALLQIASRGDVEIVYPVHLNPNVQEPVSRLLRGVEHVTLLPPLDYLPLVHLMKH
                     SNIILTDSGGIQEEAPAFGIPTLVLRDVTERPEGVDAGTLKLVGTETSHIVEETRRLL
                     EDESAYKKMATSANPYGDGHAAEKIIQALLGSRENM"
     gene            2482..>3074
                     /locus_tag="PKI33_07690"
     CDS             2482..>3074
                     /locus_tag="PKI33_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36927.1"
                     /translation="MRKTFTPIVLTLLLLAAWIPVHAQSVETQYFSETGHNVSGDFLA
                     FYNSNPNAAYLYGYPITEQITGRDGKSVQYFQRARFEYRADLPEGQRVQLTQLGRETY
                     TSTGLLDVGNSFACRNYPETGFSVCFAFLEFFDANGGVSQFGYPISGFEYHENKIVQY
                     FERARLEWQPWRADGYRVVISDLGRAYFDKLGEDPALL"
BASE COUNT          810 a          770 c          730 g          764 t
ORIGIN      
        1 ccagaagaag ccgatgcttt tattattgcg gttccaacgc cattcaaaca caatgaaact
       61 ggcaactaca acggcatgac ctacaaacta gcagatatgg aggctgtaac ttctgcagca
      121 gaatccatcg taccgtattt gaggaaggga aatgtcgtcg tcctcgaatc gacctctccg
      181 cctcgcacga cctttgactt ggttgccccc attttagaaa aatccaagct gaaagcgggc
      241 gtggactttc acctttgcta ttcaccagaa cgcgtcttgc cggggcaaat tctgcgcgag
      301 ttgattgaaa actcacgcgt tatcggcggc gtgacccctg aatctgctaa agccggagca
      361 gacttgtact ctaccttcgt caaagggcag atcattcaaa ccgatgcgac cacagccgaa
      421 atggtcaaac tgatggagaa tacttatcgc gatgtcaaca ttgccattgc taatgaattc
      481 tctcgccttg ccgataaatt cggcgtggat gtctgggaag ccatctccat tgccaacctg
      541 cacccacgtg tcaaaattct cagcccggga ccaggggttg gtggtcactg tatcagcgta
      601 gacccttggt tctttgtaga ggcggcgcca aatctcactc cgctgattta caactctcgc
      661 caagtcaacg atgcgcaacc agcctttgtt gtagatgtgg tcaaacgcgc acttggttcc
      721 ctcaaaggga aaaagatcgc cgctctcggc ttggcataca agcctgatgt ggatgattta
      781 cgtgaaagcc cggctgttga agcggtgcac tttctccaaa aagaaggcgc agaagttctc
      841 gcttgcgagc ctttcaaacc agatgccaaa ttgcccggcg taacaattgc gccaaccttt
      901 gaagatgcag ttcgtgatgc cgacgcgatt atcttgctcg taaaacatac agaatttagc
      961 aagctcaagc ctgaacaagt tgctcaacaa acaaaagcac gcctcttgat tgataccgtc
     1021 caaggttggg atgcagagca atggaagcat gcagggttcc gccatgtcaa gcttgggtct
     1081 ggaaagcccg catagttact cagcgttcag aacttctata catgtttgcg agccacctgc
     1141 aaccgcttgc ataccgcagg tggcttttgc acaagatgca agccctagaa taaggctgaa
     1201 ataaagcgcc aaaattatag aacgttttgt aaacttaatt aatttgaaca tatttgtttc
     1261 tccacacacc tagtagaaaa acaggcccaa aaaggactta tgagaatcct tacagttttt
     1321 ggaactcgac ccgaagccgt caaaatggca ccaattgtga agctgcttgc ttctacgcca
     1381 ggtgttgagg ctcgggtttg tgttacggcg caacaccgcc agatgcttga ccaagtgttg
     1441 aacctgttcc aaattgaacc acattatgat ttgaacctta tgcgagatgg acaatctctt
     1501 gcccaattaa gtgcggatat tttcacgcat ctcgaccctg ttctagaaga tttcaaacca
     1561 gactgggtgc ttggacaagg cgataccacc accgtcgcca tcacatccat gttgtgcttt
     1621 tatcgccaaa ttcgcttcgg gcatgtggaa gccggtttgc ggacccatga caagtggcag
     1681 cctttcccag aggaagtaaa ccgccgcatt gtcagcgtca tcgcggattt gcattttgcg
     1741 cccacgaatt ggtcaaagaa caatttattg cgcgaaggtg tggacgagaa aaccgttaga
     1801 gtaaccggga acactgtcat tgatgctttg ctgttcgtgt caaagcaagc cgaaccagca
     1861 gaaatcaccg aactggtgca atctaaactg agccataaaa agttaattct cgtcacggcg
     1921 catcgccgtg agaactttgg cgcgccgatg gagaatattt gtaaagcatt gctccaaatc
     1981 gcaagccgag gggatgtaga gatcgtgtat cccgttcatc tcaacccaaa tgtccaagaa
     2041 cctgttagcc gtctattgag aggcgtggag catgttactc tgctaccgcc actggattat
     2101 cttccgctcg ttcatttgat gaagcattca aacatcatat tgaccgattc aggcgggatt
     2161 caggaagaag ccccagcctt tggaatccca acactggtgc tacgcgatgt gaccgaacgc
     2221 cctgagggag tcgatgcggg gacattaaag ctcgttggaa ccgaaaccag ccacatcgtc
     2281 gaagaaacga ggcggctctt agaggatgaa tctgcataca aaaagatggc aacatccgcc
     2341 aatccatacg gagatggaca cgctgcagag aaaatcatcc aagctttact aggcagtagg
     2401 gaaaacatgt aatctttctc aagataactg tttgtaatgt cttgaggggt gtttctgagt
     2461 ataatgacag tggtggtagt aatgaggaaa actttcacgc ccattgttct gacgcttctg
     2521 ctcctggctg cttggattcc cgttcatgca cagtctgtag agactcaata tttcagtgag
     2581 acgggacata atgtctctgg agactttctt gctttttaca atagcaaccc taacgccgcc
     2641 tacctttacg gataccccat aaccgaacaa attaccggtc gcgatggaaa aagtgtgcaa
     2701 tacttccagc gtgcccgctt tgaatatcgt gccgatctgc cggaagggca acgcgttcaa
     2761 ttaacgcaac ttgggcgcga aacctatacc tccactggat tgctggatgt tggcaattcc
     2821 tttgcctgcc gcaactatcc tgagacaggc ttctcggttt gttttgcttt tcttgaattc
     2881 ttcgacgcca atggcggtgt atctcaattc gggtacccga tctctgggtt tgaatatcac
     2941 gaaaataaaa tcgttcaata ctttgaacgt gcccgccttg aatggcagcc ttggcgagcc
     3001 gacgggtatc gtgtggtgat ttcagatcta ggtcgagcct acttcgacaa gctcggcgaa
     3061 gaccctgccc tgct
//
LOCUS       DAOREF010000321         3068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21154_length_3068_cov_3.461998, whole genome shotgun sequence.
ACCESSION   DAOREF010000321 DAOREF010000000
VERSION     DAOREF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21154_length_3068_cov_3.461998"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1307)
                     /locus_tag="PKI33_07695"
     CDS             complement(51..1307)
                     /locus_tag="PKI33_07695"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM36928.1"
                     /translation="MSKKYSPFILLLIVLLTVQLACNLPSNSATPDPFATLNGLYTAS
                     AQTQAVAGSPTPGLPQPTVTLAGSPTATNPPVTLQPAQTSRCDAAQFLADVTYPDGSL
                     VPRNTTFVKIWRIRNVGTCTWSTSYSVVFTGGDGMGASTSIGMPGSVAPGQYIEIPVT
                     MVSPSKDGNYRGYWKIRNSSGALFGIGGQADTAFWVDIRVTGPSFVAYNFAENYCQAN
                     WSNGSNALPCPSSNGDANGYIIRLNAPVMENGVTEDEPGLLTVPQDKNNGIISGQFPS
                     FTVQSGDRFRTIVNCQRDATKCNVVFRLDYLNNGVTSTYASWAEAYEGKYYPVDLDLS
                     SLAGQTLKFILVVSANGGNNQDFAIWLNPHIVRQGVAPTSTNTPTPTITFTPTLTFTP
                     TITSTATTTSTPTVTATPTETPTPTP"
     gene            complement(1408..2367)
                     /locus_tag="PKI33_07700"
     CDS             complement(1408..2367)
                     /locus_tag="PKI33_07700"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM36929.1"
                     /translation="MLKKKIYTLSALFFTAALLLTACGGNVDAETAIQTSVAETVAAQ
                     TPIVITETPAATLFPTQTPFTTPTLGTPIALVSPTTGAVTGGKAECAKASLQDETVVD
                     GKIFKPGEEFVKTWYIKNESTCVWDTTYKIIFWSGDILGGGYYYQLPQVTGPGQTVPV
                     SLVLTAPTTPGEYRSEWKLQTPDSIPYGVGVYQAAFYTEIEVANLTTTTAITYGIVDA
                     NLVITRDPPSGCQPANMVYTANVSITTNGPLTFKYTIRQQDGNSGNSTKVVMKEAGEY
                     TNSDHVWKLGRAASQNSNRWMQLVITDPVYKEYPIVPFDFYCP"
     gene            complement(2456..>3068)
                     /locus_tag="PKI33_07705"
     CDS             complement(2456..>3068)
                     /locus_tag="PKI33_07705"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM36930.1"
                     /translation="MTPGQSFTKKWRIRNTGECAWNGYNLVFDTGDSMGGPASKAVGV
                     VNPGQEIDIEVNLTAPASAGNYRGYWRIVTNNNVLVPMLNGYQGRAFYVDIKVQAPAT
                     STNTSAPAFAVTSVVFEVTGTCPNFHVKVSVTTSAAGTVKYKFVSQDGSSTSLADLVF
                     AAAGTKVVEYDRFFGVSPGSLWTDMYVDQPNNQQFGRATFVCP"
BASE COUNT          765 a          661 c          793 g          849 t
ORIGIN      
        1 agaggcaggt tgtacagtgc ttctgtgacg ttttatgttt ttagattaat ttacggtgta
       61 ggcgtaggtg tttcggttgg tgttgcagtg acagtaggtg tagacgtagt tgtagcggtt
      121 gatgtaatgg tgggtgtaaa ggttagtgta ggcgtaaatg taatagtagg cgttggcgta
      181 ttcgtagatg ttggtgctac accctgccga acaatatgcg gattcaacca aatggcaaaa
      241 tcttggttgt taccaccatt ggcgctgacg accaagataa actttagtgt ttgcccggca
      301 agactgctga gatccaagtc tacgggatag tattttcctt catacgcctc tgcccaactg
      361 gcataggtgc ttgtaacgcc attgtttaag tagtcaagcc gaaatactac attacatttc
      421 gtcgcatcgc gttgacagtt aacaatggtg cggaatcggt ctcccgattg aaccgtaaac
      481 gaaggaaatt gcccactgat aatgccattg tttttatctt gtggcaccgt caacagaccg
      541 ggttcatctt cagtaacgcc attttccatt acgggggcat ttaaccggat gatatacccg
      601 tttgcatctc catttgagct tgggcagggc aaagcattgg agccgttcga ccaatttgcc
      661 tgacaataat tttctgcaaa attataagca acgaaagatg gtcccgttac acggatatct
      721 acccagaaag cggtatctgc ctgcccgccg attccaaata atgctccgct tgaatttctt
      781 attttccaat acccacgata gttcccatcc ttgctgggcg agaccatcgt aacaggaatt
      841 tcaatgtatt gtccgggtgc tacacttccg ggcatgccaa ttgaagtaga tgcccccata
      901 ccgtccccgc cagtgaaaac aacggaatag gatgtagacc atgtacacgt tcccacattg
      961 cggatgcgcc atattttgac gaaggttgta ttacgtggca cgagactgcc atctggataa
     1021 gtcacatcgg ctagaaattg cgccgcatca cacctcgacg tttgggctgg ttgcagcgtc
     1081 actggcggat tcgtagctgt gggcgagcca gctagagtca ctgtcggttg gggcaagcct
     1141 ggggttggcg agcccgctac cgcctgagtc tgagccgagg cggtgtagag tccgtttagg
     1201 gtggcgaagg ggtctggggt ggcggaattc gagggcagat tacaggcaag ttgaacagtc
     1261 aataaaacaa tgagtaaaag gatgaatggt gagtactttt tggacatgct tcctccattt
     1321 ctttcaacca ttaaagacga ataacctatg gatctggttc ccaataaaaa gaagccggta
     1381 tgcaccggct tctttgaaaa atttatgtta cgggcagtag aaatcgaacg gtacgattgg
     1441 atattcctta tagacaggat ccgtgattac aagctgcatc cagcgattac tattttgtga
     1501 tgcggcacgt cccaatttcc atacatggtc agagttggta tattcgccag cttctttcat
     1561 gacaactttt gtgctattac cgctattccc atcttgttgg cgaatggtgt atttgaatgt
     1621 taagggacca tttgtcgtga tgctaacgtt ggctgtataa accatgttcg caggctgaca
     1681 cccagatggc gggtcgcgag tgatgaccag gtttgcatcc acaatgccat aagtaatagc
     1741 tgttgtggtt gtaagattgg cgacttcgat ttcagtgtaa aacgcagctt gataaacccc
     1801 tacaccgtaa gggatactat ctggggtttg caatttccat tcggagcgat attcaccagg
     1861 ggttgttggc gcagtaagga ctaaagaaac cggtaccgtt tgacccggac ctgtcacttg
     1921 gggtaactga taataatatc cgccgccgag aatatcacca gaccagaaaa tgattttgta
     1981 ggtggtatcc caaacgcagg tgctttcgtt cttgatatac caggttttga caaattcttc
     2041 accgggttta aatattttgc catccacaac ggtttcatct tggaggctgg cttttgcaca
     2101 ttcagctttc ccgccggtga cggcaccggt ggtgggggat acgagcgcaa tgggtgtacc
     2161 cagtgtgggt gtagtaaaag gtgtttgagt tgggaacaag gtggcggctg gagtttcagt
     2221 aattacgatc ggagtttgag ccgcaactgt ttctgccact gatgtttgaa tggcagtttc
     2281 agcatctaca ttgccgccac aggctgtgag gagcaatgca gcggtgaaaa atagcgcaga
     2341 gagtgtgtag atttttttct tgagcatttt tttcctttca aatacagaaa atgattatac
     2401 tgttaaatga caacgcccgt attcacgggc gttgtgggat taatgattta ttattttatg
     2461 gacaaacgaa tgttgctcgt ccaaattgct ggttgttggg ctgatctaca tacatatctg
     2521 tccaaagaga accagggctg accccaaaga agcgatcata ttccactact tttgtgcctg
     2581 ctgcagcgaa tactagatct gctaaggatg tggatgagcc atcttgagag acgaacttgt
     2641 actttacggt ccctgctgca ctggtggtaa cgctgacttt aacgtggaag ttcgggcatg
     2701 ttcctgttac ttcgaaaacc acacttgtga cagcgaatgc aggggcgctg gtgttagtag
     2761 acgtagctgg ggcttggacc ttaatgtcaa cataaaaggc tctgccctga tatccattaa
     2821 gcatagggac gagaacattg ttgttggtga cgatacgcca gtatccgcga taatttccag
     2881 cagaagcagg ggcggtgaga ttaacttcta tgtcaatttc ctgaccgggg ttcacgacac
     2941 ccacggcttt agaggcgggt ccgcccatgg aatcaccggt atcaaagaca aggttgtagc
     3001 cgttccaagc acattcaccc gtattacgaa tacgccactt cttcgtgaag gattgtcccg
     3061 gggtcatc
//
LOCUS       DAOREF010000322         3061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21234_length_3061_cov_4.505655, whole genome shotgun sequence.
ACCESSION   DAOREF010000322 DAOREF010000000
VERSION     DAOREF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21234_length_3061_cov_4.505655"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PKI33_07710"
     CDS             <1..1413
                     /locus_tag="PKI33_07710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM36931.1"
                     /translation="MFLSMVKDLQTSHYVLPKFTTYNYLNIPFAYPPFGFYFARAVSD
                     ILSIPELALFRWVPVLVNSFSIFVFYLLASEIAKSKLAGAISSVLYALTPGAFGWFIM
                     GGGLTRSFGSLFLLLTVYSTLKLFREDEKKYIGLSILFGGLTVTSHPEAGIHAAIGCV
                     LVWVFFGRTNKSLLQAIAIGLGVFLFISPWLASVLHYHSVTPILSALHTGSSGNNFIG
                     SIYDLLFDEGIIPILFLFRITGLIWGVSKKHYFLLVWSFLPYLVEPRSAPSVSFYPMT
                     ILTALAFIEALPWLLGGIKKQEFQNLHQNVIFNIFLLTVISLLFIDSCLYGFRLVGNS
                     LKPNDLETMYWVKENLPENSVFMVLTGIPSPEIDPYVEWFPALAERQNISTLQGYEWL
                     LADKFYNFYTELSKLQQCRSVDCIEKWNTTNSQRYDYVVIFRTEAVEIDELLLKNSSY
                     EKVYSNSNALIVKYDESSTK"
     gene            complement(1426..1845)
                     /locus_tag="PKI33_07715"
     CDS             complement(1426..1845)
                     /locus_tag="PKI33_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNM36932.1"
                     /translation="MNCPKCSSELTKKYYKGMFEVDSCPNCRGMWLDFNELDKLEDVI
                     FDEDEHKGSLVHFQTRTEFPCPHCGMLLDEFKYRLYDLKLETCAENNHGFWLDAGEDE
                     RVMEIMQKRAGDMQRKINAETEWKQILKGMHEFLGRK"
     gene            complement(1826..2635)
                     /locus_tag="PKI33_07720"
     CDS             complement(1826..2635)
                     /locus_tag="PKI33_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNM36933.1"
                     /translation="MPKVRLDVLLVEKGLADSRAKAQALIMAGQVRVAGQVALKPATT
                     VQPDATLTVDMGPKFVSRGGEKIEGALDAFKIDVTGFTCADVGSSTGGFTDCLLQRGA
                     VKVYAIDVGKGILHWKLRNDPRVVVMEETNARFVESLPEKIDFVSVDASFISLKVLLP
                     VIKKWNVEQGILALIKPQFEAGKKEVSRGDGVIRDPEIHKQVLLDVLHYAKAEGFGLL
                     GLVKSSLLGPKGNTEFLVWLNMKPNDVLAEDLVNSIFPASEMDKNDELSKV"
     gene            2685..>3061
                     /locus_tag="PKI33_07725"
     CDS             2685..>3061
                     /locus_tag="PKI33_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36934.1"
                     /translation="MKHLFLLSFTLVIILGCISAPLTAAPEEPTVQVSVPTIEPTRET
                     LARPFPQHTSYAPDSILPNHRTQEQLDDDVRTYYDYWKSTYIAEAGTNADGVVMYRVV
                     LGKDEPTRSTTVSEGQGYGMMIVP"
BASE COUNT          834 a          686 c          596 g          945 t
ORIGIN      
        1 atgtttctaa gcatggttaa agatctacag accagtcact atgttcttcc aaaattcact
       61 acatataatt atctaaacat accttttgca tacccacctt ttgggtttta ctttgcgagg
      121 gcagtttcag atatcctttc cattccagaa ttagccttat ttcgttgggt gccagtactg
      181 gttaattctt tttcgatttt tgttttttac ttattggcat ccgagatagc caaatcaaaa
      241 ctagcagggg caatctcgtc tgtgctctat gcattgacgc ccggcgcatt tggttggttt
      301 attatgggcg gagggctaac acgcagtttt ggctctttat ttttgctttt aactgtctac
      361 tccacactaa agttatttcg agaggatgag aaaaaatata tcggtttatc gatattattt
      421 ggcggtttaa ccgttacgag tcacccagaa gcggggatcc atgccgctat aggctgtgtt
      481 cttgtgtggg ttttctttgg tcgaacaaac aaatcccttt tgcaagctat tgcaattggg
      541 ctaggcgttt ttcttttcat atctccatgg ctagcaagtg tattgcacta tcatagtgtt
      601 actcctatcc tttcggctct acatacggga tccagtggga ataattttat cggctcaatc
      661 tatgacctat tatttgacga aggtattatt ccgatcttgt ttctttttcg tattacaggc
      721 ttaatatggg gagtttcaaa aaaacattac ttccttttgg tatggtcttt tttaccttat
      781 cttgttgagc cacgcagcgc gccaagcgta tcgttctatc caatgacaat actaacagcg
      841 ctggcgttta ttgaagcttt gccgtggcta cttggaggaa taaaaaaaca agagtttcaa
      901 aatttgcatc aaaatgttat cttcaacatt tttctactta ccgtcatctc tttgttattt
      961 attgattcat gcttatatgg atttcgtctt gttgggaata gcctcaaacc aaacgacttg
     1021 gaaacaatgt attgggtaaa agaaaattta cctgaaaatt cagtgtttat ggtcttaacg
     1081 gggattccca gcccagaaat cgacccatat gtagagtggt tcccggcatt agctgaacgt
     1141 cagaatatct ccacattaca aggttatgaa tggctactgg cagataaatt ttataatttt
     1201 tataccgagc tttcaaaact acagcaatgc agatcggttg attgtataga aaagtggaac
     1261 acgaccaatt cacaacgata cgactatgtt gttattttta gaacagaggc agtcgaaatt
     1321 gatgagctat tgttgaagaa ctcaagttat gaaaaagtct actcaaacag caacgcatta
     1381 atcgtcaagt atgacgaatc atctacaaaa tagctaagat cttttctatt ttcttcctaa
     1441 aaattcgtgc atacccttga ggatttgctt ccattccgtc tcggcgttga tttttcgttg
     1501 catatccccc gctcgctttt gcatgatctc catcacacgt tcgtcttcac ccgcatctag
     1561 ccaaaaaccg tgattatttt ctgcacaggt ttctagcttt aagtcatata aacgatactt
     1621 aaattcgtcc aatagcatac cacagtgcgg gcagggaaac tccgtccgtg tttggaagtg
     1681 aactagtgac cctttgtgtt cgtcttcatc gaagatcaca tcttcgagtt tatcaagttc
     1741 gttgaagtcc agccacatgc cgcggcagtt tgggcaggaa tccacttcaa acattccttt
     1801 gtagtacttc ttcgttaatt cagaactaca ctttggacag ttcatcgttc ttatccattt
     1861 cgcttgcagg gaagatgcta ttaaccaaat cctctgcaag gacatcattc ggtttcatat
     1921 tgagccagac caaaaattcg gtatttcctt ttggtcccaa caacgaagat tttaccaacc
     1981 caagcaagcc aaagccttct gcttttgcat agtgtaagac atccaataaa acttgtttat
     2041 gaatttccgg gtcgcggata acgccatcac cacgcgaaac ttcttttttg ccagcttcaa
     2101 attgcggctt gataagcgcc agaattcctt gttccacatt ccattttttg atgacaggca
     2161 ataaaacctt caacgatata aaagaagcat ctactgatac aaaatctatc ttctcaggta
     2221 aagactctac aaaacgcgca ttagtttctt ccatcactac aacacgtgga tcattacgca
     2281 gtttccaatg cagaatgccc tttcccacat caatggcata gacttttacc gctccacgct
     2341 gtaagagaca atcagtaaag ccgcctgtcg atgatcctac atcagcacag gtaaaacctg
     2401 tgacatcgat cttaaaggca tccaatgcgc cttcgatctt ctcgcccccg cgcgatacga
     2461 atttgggtcc catatccact gtcagcgtgg catcaggttg aacagtagtg gcaggcttga
     2521 gcgcaacttg acccgcgacg cgcacttgtc cagccatgat gagggcttgg gctttggcac
     2581 gcgagtcagc caatcctttt tctacaagca gaacatccaa acgaacttta ggcatagctc
     2641 tattttactt ccagagatga tataaaagag gaaatagagg atatatgaag catctttttc
     2701 ttttaagttt tacgcttgtg attattttag gctgtatctc tgccccgcta acagcggcac
     2761 ccgaggagcc taccgtacag gtttcagtac cgactataga accgactcgt gaaactcttg
     2821 cgcgaccgtt cccacaacat acatcgtatg cccctgatag tattttgcca aaccaccgca
     2881 cacaagagca gttggatgat gatgtgcgca cttattatga ttactggaaa tcgacataca
     2941 ttgccgaagc agggacaaac gcagatggtg tcgtaatgta tcgtgtggta ttaggcaaag
     3001 atgagccaac tcgttctaca acggtttcag aaggacaagg atatggcatg atgatcgtac
     3061 c
//
LOCUS       DAOREF010000323         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21292_length_3056_cov_4.139287, whole genome shotgun sequence.
ACCESSION   DAOREF010000323 DAOREF010000000
VERSION     DAOREF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21292_length_3056_cov_4.139287"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKI33_07730"
     CDS             <1..773
                     /locus_tag="PKI33_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNM36935.1"
                     /translation="DDYALHLYIGTSSWMAAHVPFKKTDVASSLASIPCAVPGRYLLT
                     ALQATAGGNLTFLRDNIIYHKDELLQEADVPDIFKVLDQIANRVPAGANGVMYTPWIW
                     GERAPVEDKTLRAGLYNLSLNSTREDIIRAFLEGIAYNTRWLLSPVEKFLGRKVNSIN
                     IVGGGAQSDLWCQIFADVMNVEIKQVADPIYANARGAAWIAAVGLGEIKFADIPELMQ
                     FRRAYQPQVQNRAVYESGFENFQLIYKQMKGVYRRLNQ"
     gene            complement(777..2285)
                     /locus_tag="PKI33_07735"
     CDS             complement(777..2285)
                     /locus_tag="PKI33_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM36936.1"
                     /translation="MSIRLRITFLYTFILALTLTVFGLALYTIQAQSTMDSLKHDLSV
                     SANRLADATLKTDFPNFNGNPPPNGQEPPNGQPPNGQEPPPGGQQPGDGNPPPPPIPF
                     DQFSNQQEFQSFSEREIARVLDVQGNLVSSPFGREGDALPLSAAGLQALQNGSDWWET
                     NIVSDESMLTYNRPVIVDNKVIYIVQVARSLTERNRTLQSLSTTLLLAGLLTTLIAFG
                     IGWLFSGLTLAPINRITKTAKEIGEERDFSRRVDYNGPQDEVGQLASTFNAMLTRLQD
                     AFKKVEHSLQMQRNFVADVSHELRTPLTTLRGNLGLLKRNPSPEIQNDILSDMVDESD
                     RLIRLVNDLLLLARADAGRSLSKEPVDVSIVLQEVCRQGNQLDANREINLDVPADTSI
                     IGDRDAFKQVALILLDNALKHSTGKINIRAEKYGKHIDVRVQDFGEGIPPETLAHVFD
                     RFYRGEDAATIPGFGLGLPIAKTLVEGFGGEIKIESEVGKGSTVIMRFPSAK"
     gene            complement(2285..2965)
                     /locus_tag="PKI33_07740"
     CDS             complement(2285..2965)
                     /locus_tag="PKI33_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM36937.1"
                     /translation="MPSVLVIDDDPKLLKMLQRTLTYENLEVFIANNGLEALPLVQAQ
                     KPDLIIVDWMMPKMDGISFIQRLRDEENQTMILMLTAKDAIDDRVDGLESGADDYLIK
                     PFAPAELVARVHAMLRRVETKPENQKVSYADVSLDPTSREAKRGTIELNLTVTEFNLL
                     HMLLRHPRQVLERRQILNEVWGYDFGGDDNVLEIYIGYLRKKLEVDGNSRLIQTVRGV
                     GYVLREES"
BASE COUNT          740 a          696 c          796 g          824 t
ORIGIN      
        1 tggacgatta cgcgctgcat ctttacattg gcacatcgtc atggatggcg gcgcatgtgc
       61 cgttcaagaa aacggatgtg gcttcaagcc tggcgtcgat tccctgtgcg gtgccggggc
      121 gctatctgct cacggcgctg caagccacgg cgggcggcaa cctcactttt ctgcgcgaca
      181 acatcatcta tcacaaggat gaattgctgc aagaagccga tgtgcccgat attttcaagg
      241 tgctcgacca gatcgcaaac cgtgtccctg ccggtgcgaa cggagtgatg tacacgccat
      301 ggatttgggg agagcgtgcg ccagtggaag acaaaacgct tcgtgccgga ctttataact
      361 tgtcactcaa ctccacacgt gaggatatca tccgcgcctt tttggaaggc attgcttata
      421 acacacgctg gttgctatcc cctgtggaaa aattcctggg tcgcaaagtg aatagcatta
      481 acatcgttgg cgggggagcg cagtccgacc tgtggtgtca gatttttgcg gatgtgatga
      541 acgttgagat caagcaggtt gcagacccca tctatgccaa tgcgcgcggc gcggcatgga
      601 ttgcggcggt aggcttgggc gaaattaagt ttgccgatat tccagagttg atgcagttta
      661 gaagggcgta tcaaccccaa gtccagaacc gagccgtata cgaatccgga tttgaaaact
      721 tccaattgat ttataaacag atgaagggcg tgtatcgtcg tttgaatcag taaagtctac
      781 ttggcagatg gaaagcgcat gatgactgtg ctgcctttgc cgacttcgct ttctattttg
      841 atctcgccgc caaagccttc aacaagagtt tttgcgattg gcagacccag cccaaacccc
      901 ggaatggttg cggcatcttc cccgcgatag aaacggtcga agacatgcgc aagggtctcg
      961 ggtggaatcc cttcgccgaa gtcttgaacg cgaacgtcaa tgtgctttcc atatttttca
     1021 gcgcgaatgt tgatcttgcc tgtggagtgc ttaagtgcat tatctagcag gatcagtgcg
     1081 acctgtttga aggcatcacg atctccaata atcgaagtgt cagcggggac atctaggtta
     1141 atctctctat ttgcgtcaag ctgatttccc tgcctgcata cttcttgaag aacaatagaa
     1201 acatccactg gttctttcga gagactacgc ccggcatcgg cgcgtgcaag caaaagcagg
     1261 tcgttgacga ggcgaataag acgatcgctt tcatcgacca tatcgctaag aatatcattt
     1321 tggatctctg gcgaaggatt gcgtttgagc agcccaagat ttccgcgcaa tgtggtaagc
     1381 ggtgtgcgca attcgtgtga gacgtcggca acaaagtttc gctgcatttg cagagagtgc
     1441 tctacttttt taaatgcatc ttgcaagcgg gtaagcatgg cattgaaggt gctcgcgagt
     1501 tgaccaactt catcttgcgg accgttgtaa tcgacccggc gagaaaaatc tcgctcttca
     1561 ccgatctctt tggcagtttt ggtgatgcgg ttgatggggg ctaatgtaag acctgaaaaa
     1621 agccagccaa ttccaaaagc aatcagtgtt gtaagtaaac ctgcaagcaa taatgttgta
     1681 gataaagatt gcagtgtgcg atttcgttcg gtgagtgaac gagctacctg aacgatataa
     1741 ataactttat tatctacaat gacggggcgg ttgtaggtaa gcatgctttc atctgaaaca
     1801 atgtttgttt cccaccagtc gctgccgttt tgtaaggctt gcaacccagc ggcgcttaaa
     1861 ggcaatgcgt ccccttcgcg tccaaagggg ctgctgacca gattaccttg aacatctagg
     1921 actctggcga tttcacgttc tgagaacgat tgaaattctt gttgattgga aaattgatcg
     1981 aatggtatgg ggggaggagg tgggtttcca tctccgggtt gttgcccacc tggtggaggt
     2041 tcttgtccgt tgggtggttg tccatttgga ggctcttgcc cgttcggagg agggttgcca
     2101 ttgaaattgg ggaaatccgt tttcagtgtt gcatctgcta aacggttggc gctcacgctt
     2161 aggtcatgtt tgagcgaatc cattgtcgat tgtgcttgaa ttgtgtatag cgccagacca
     2221 aagacggtta acgtcaacgc gaggatgaat gtataaagaa aagtgatgcg aagccgaatg
     2281 gacattatga ttcttcacgc agaacgtagc caaccccgcg gacggtttgt attaggcgcg
     2341 aattcccgtc tacttcaagt tttttacgca gatatccgat atagatctca agaacgttat
     2401 catctccgcc aaagtcgtag ccccagacct cattgagaat ctgacgccgt tcaagcacct
     2461 ggcgtggatg ccgcaatagc atatgcaaaa gattgaattc cgtcaccgtg agattgagtt
     2521 cgatggtacc acgtttcgcc tcgcgggatg tggggtcgag gctgacatct gcataagaga
     2581 ctttctgatt ttctggtttt gtttctactc tgcgcagcat ggcatgaacc ctcgcaacca
     2641 gttctgccgg ggcgaaaggt ttaattagat agtcatcagc gccgctttcc aacccgtcta
     2701 cacggtcgtc aatggcatct ttcgctgtga gcatcagaat catggtttga ttttcttcat
     2761 ctcgtaaccg ttggataaaa gagatgccgt ccattttggg catcatccaa tctacaatga
     2821 tcaggtctgg tttttgcgct tgaacaaggg gaagggcttc cagtccatta ttggctatga
     2881 aaacttcgag gttttcatag gtcaatgttc gctgaagcat ctttaatagt ttggggtcat
     2941 catcaataac aaggacagag ggcataggat ctcgcttttt tagcttactg caccaactat
     3001 aaatgaaatt cttaagctga gaatgtactc acagcatatt cacagtttct tctttg
//
LOCUS       DAOREF010000324         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21348_length_3052_cov_3.811812, whole genome shotgun sequence.
ACCESSION   DAOREF010000324 DAOREF010000000
VERSION     DAOREF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21348_length_3052_cov_3.811812"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..614)
                     /locus_tag="PKI33_07745"
     CDS             complement(258..614)
                     /locus_tag="PKI33_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36938.1"
                     /translation="MNKSLIPFFLPFIIVACGSKGDEVPDVLLTPPAIVVNASRDNCP
                     SIEAEVGMTIAWENVDTVPLPIKIEFLDDNGKVTDTGLSVIDPETVFSTQFQEAGVYH
                     VYCTENADTYSTITVK"
     gene            complement(674..2194)
                     /locus_tag="PKI33_07750"
     CDS             complement(674..2194)
                     /locus_tag="PKI33_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HNM36939.1"
                     /translation="MNDQNPSPEFEEKVREAMHMPEADPKFAKRLRNELVGRPVKMKT
                     KIFRPAWAIAFVLALVVLAINVPRLAAALGRVFGYIPEVGLVETTNGLRMLAEPISST
                     RDGVTITITNVFAYPDRIELQYEVSGVAPEDDGWQSSDASSNPTAFCGGVNPGSTYNK
                     AGDARLRLPDGTVLERDYTGKYPKNVFAMQPVYDGALPAGVTSLTFMLDCIPQARLGV
                     VPEHWELALDLVEVPAGTVVGEPVIEVTQPVVTEEAPASDSTETVSAPKVTITLERIV
                     PTDTNTVVYFHLNMENADPSLVSIMPRSVYVIDSLGQQFRMVGSFVWQPFEHKVGSAF
                     EFVSEGKPATGPLTVVVDEMTAYYMPMYVDPPQATPEEMTFTFDVGENPQYGQKWDLN
                     KTFTIAGYDFEIVSAQASNYDDVAKIHDYIDGSQGYEYGYQFAVAADPSLELLVEMDI
                     RSDEYQCWLSDVNKVETQPLLYTQLCKDGYPKGQVSVTIREMSIALDDDVQAEWIP"
     gene            complement(2197..2754)
                     /locus_tag="PKI33_07755"
     CDS             complement(2197..2754)
                     /locus_tag="PKI33_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM36940.1"
                     /translation="MLGFEAVKTKLMSQDEISELISAAKSDPTAFGRLYDRYLQPVYR
                     YLYSRLGDAHSAEDVTSQTFITAYEALPKYREQGQFTAWLFRIARSKMYDYLRKNKRE
                     VELEAAGELLEREDALGTLIRAEELSKVRFLIKQLNEEEQDLIRLRYVADLTFVEIAD
                     LLGKREDAVKKTLYRLLARLKGQME"
BASE COUNT          817 a          752 c          684 g          799 t
ORIGIN      
        1 tttggtcggc acgatcatca tctatgcctt tggcgtcact tggttgagct ccgtcgtcgg
       61 cggttttggt aaagctatcg aattaggatt gctaccattt gtggttggtg atgctatcaa
      121 gttagtcgct gctgctgtag cattgcctgc agcttggaaa ttcgtccgat aactaaaaac
      181 atcacacgcc tagaataaga caattattta ctccccatac acctgcatat attcttgcag
      241 gcgtatgggg agttttttta tttcaccgta atcgttgaat aggtgtcagc attttcagta
      301 cagtagacat gataaacccc ggcttcttga aattgtgttg aaaacacggt ttcagggtcg
      361 atcacagaca atcccgtgtc agtaaccttt ccattgtcat ccaagaattc gatctttatc
      421 ggcagaggca ccgtatcgac attttcccaa gctatggtca ttcctacctc tgcttctatg
      481 gaaggacagt tgtcccgcga agcgttcaca acaatggcag gcggagtgag gagcacatct
      541 ggcacttcat caccttttga accgcaggcg acaataatga aaggcagaaa aaaggggatt
      601 aaacttttat tcatcatatt ctcctgcaaa gcttttaaca tcgaaaggaa ttttcctgaa
      661 tggtataaag ttcctatggt atccattcgg cttgaacatc atcatccaac gcaatggaca
      721 tttcgcggat ggtgacagaa acctgtcctt tcgggtaccc atccttacaa agttgggtat
      781 agagcaaagg ttgagtttcc actttattga catctgacag ccagcattgg tattcgtcac
      841 ttcgaatgtc catttcaacc aaaagttcca aagatgggtc ggcagcaacg gcaaattggt
      901 agccatactc atatccttgc gaaccgtcta tataatcatg gattttcgcg acatcatcat
      961 agttcgaagc ttgggcagag acgatctcaa agtcgtaccc ggcaatggtg aaagtcttgt
     1021 tcaaatccca tttctgaccg tattgcggat tctcgcccac atcaaaagta aatgtcattt
     1081 cttcaggggt tgcctgtgga gggtccacat acatgggcat gtaataggcg gtcatttcat
     1141 cgaccaccac ggtcaaggga ccggtagcag gtttgccttc gctgacaaat tcaaaggcac
     1201 tgcccacttt atgctcaaaa ggctgccaaa cgaatgaacc aaccatacgg aattgttgac
     1261 cgagcgagtc gatgacgtaa acactgcgcg gcatgatgga gaccaaagaa ggatcggcat
     1321 tctccatgtt taggtggaag taaacgactg tgttggtatc agtagggacg atccgttcga
     1381 gggttatggt cactttggga gcagagacag tttcggttga gtcagaggcg ggcgcttctt
     1441 ctgtcacaac aggttgagtc acttcgatca ctggctcgcc cacgactgtt ccagccggga
     1501 cttcgacaag atcaagcgcg agttcccaat gttcaggcac aacgccgaga cgggcttgcg
     1561 gaatacagtc gagcatgaag gtgagagaag tgacacccgc cggaagcgca ccatcataaa
     1621 caggctgcat cgcaaaaaca ttcttcggat atttgccggt gtaatcacgt tcaagcaccg
     1681 tgccatcggg cagacgcaaa cgcgcatcgc ccgctttgtt atatgtactg ccaggattca
     1741 cgccgccgca aaatgcagtc gggttcgacg atgcatcaga tgactgccag ccgtcatctt
     1801 caggagcaac gccactcact tcatattgca attcaatacg atcaggataa gcaaatacat
     1861 ttgtgatcgt aatcgtgacg ccatcacgag ttgatgaaat cggttctgct aacatacgta
     1921 aaccattcgt tgtttccacc aaccccactt cgggaatata tccaaacaca cgacctaatg
     1981 cagcagccaa tctcggaaca tttattgcaa gaactactag agcaagcacg aatgctatcg
     2041 cccaagcggg tctaaatatt tttgttttca tttttactgg tctccctacc agttcattgc
     2101 gtaatcgctt tgcaaattta gggtccgctt caggcatatg cattgcttcg cgaaccttct
     2161 cttcaaattc aggagaggga ttttgatcgt tcatattcac tccatctgac ccttcaaacg
     2221 agccaacaaa cggtaaagag tctttttgac ggcgtcttcg cgcttgccaa gcaaatctgc
     2281 aatctcaacg aaggttaggt cagccacata gcgcaagcga atcaaatcct gttcttcctc
     2341 gttcaattgc ttgatgagaa aacggacttt gcttaattct tccgcgcgga tcaacgttcc
     2401 cagtgcgtct tcacgctcaa gcaactcgcc cgccgcctcc aactcgacct cgcgcttgtt
     2461 cttacgcaag tagtcataca tcttgctgcg agcgattcga aataaccacg ccgtgaattg
     2521 accttgctct cgatacttgg gcaacgcttc atatgcagtg atgaatgtct gcgatgtaac
     2581 atcttctgca gaatgagcat caccaaggcg actatagaga tagcgataga caggttgcag
     2641 ataacggtca tacaacctgc caaacgcggt ggggtcgctt ttggctgcgg atatcagttc
     2701 ggagatttcg tcttgactca ttaatttcgt ctttactgct tcaaatccta acatgcagtt
     2761 cctttctctc gcgaggatgc gtatgtaaat gcattcataa agtaaacgca ccgaaaatca
     2821 aaacaggaca aaactgtcat gccaaaatca ggctaaacgt tacttcacca gagaggtaat
     2881 ttcttttaga ttagatatta agcatcgtaa atgtcatcgt gggtaaaaac ggacaggctc
     2941 aaatcccacc tcgagtttgc cggacattgc cccacaagcg ggacaggttc ctcaccgtga
     3001 catgcacagg agtttcctat gccgatcaaa accatcatca tgggcgccgc ag
//
LOCUS       DAOREF010000325         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21470_length_3043_cov_4.562249, whole genome shotgun sequence.
ACCESSION   DAOREF010000325 DAOREF010000000
VERSION     DAOREF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21470_length_3043_cov_4.562249"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKI33_07760"
     CDS             complement(<1..189)
                     /locus_tag="PKI33_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNM36941.1"
                     /translation="MVTPSLKQEISQLEANFCAALSDPIRLLILYTLNDGSRNVTELA
                     TELGINQPTTSRHLKILRE"
     gene            348..953
                     /locus_tag="PKI33_07765"
     CDS             348..953
                     /locus_tag="PKI33_07765"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNM36942.1"
                     /translation="MEPYQREAISVDVSVRLRELREARGISMRTLASRSGLSANALSM
                     IERGKTSPSVSTLYKLANALGVSITAFFDTETEKRQVVFLKSDGRTRLSFTRGVFEAL
                     GGENFVGRVEPFMLTMESGASCGPHDIVHSGHEFVFCLRGQLDYTVEKETFRLEPGDS
                     LLFASKLRHRWKNPANVVANALIIISGFAEGEEPHSMHWNK"
     gene            complement(1016..1363)
                     /locus_tag="PKI33_07770"
     CDS             complement(1016..1363)
                     /locus_tag="PKI33_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNM36943.1"
                     /translation="MSDSQINEIQWTIHTTNPDLVKQARASLSEVVDPEIGMNIIQLG
                     LVRDIEVENGMARLKMILTTPFCPYGPAMIEMTKAKATEGLNMPVTVEMGMDVWDFSM
                     MEDPSALDWGMYA"
     gene            complement(1376..1999)
                     /locus_tag="PKI33_07775"
     CDS             complement(1376..1999)
                     /locus_tag="PKI33_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNM36944.1"
                     /translation="MKSTRDKILQTLLKKPKSTINDLADAVGINPISVRHHLTNLQME
                     GLVESQEERHGVGRPRLVYVLTDEGMERFPTRYLRLTTRLIAQMKESIPGPMVSKLFG
                     QIAEDLANEYANDIKGLSMEERLDFVKDMLAQEGFTVEWEKKDGQYQIHEISCPYYQI
                     GVAHPEVCTVDQTLISKMLALPANKVRCILDGGAHCTYVVQPVGKTK"
     gene            complement(2071..>3043)
                     /locus_tag="PKI33_07780"
     CDS             complement(2071..>3043)
                     /locus_tag="PKI33_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36945.1"
                     /translation="NAQGIPLGGNGIGNANFTTGETYLIDRISPQVTSIIRAGGNPAI
                     NPTADFIVTFSEPVNGVETSDFILTSSNVIQSSVVGMQNADPFYWVKVSTGIGSGSIR
                     LDLLDNGNITDKAGNPLLNRNFTGESFNIAKTPVDFSAPTMNSVQGKLTNNPLVSISW
                     SAVRNAQAYEVFVARDSNFSQIVFIQTTTSTSITPPTPLADGGYYVRVRAYNPDLNPG
                     KFSKAINFAVDATPPAAPNLLSPASSATTAKRVWLKWSVVNSVVKYQIQVDNNSDFSS
                     PEFDATTNKATIQTKSLPARTYYWRVRGIDSAGNWSAWSQTFLFTTK"
BASE COUNT          726 a          646 c          774 g          897 t
ORIGIN      
        1 ctcacgcaat atcttgaggt gacgagaggt ggtgggttgg ttgatgccga gttctgttgc
       61 cagctcggta acattacgtg aaccatcatt gagggtgtat agaataagga ggcggatagg
      121 gtctgaaaga gcggcgcaga aatttgcttc aagttgtgag atttcttgtt ttaagctggg
      181 agtaaccata aacaccttct tatatgcata taaacgaata tttatacttg catcataata
      241 ttgtgacagt ttgcacaata gtggagggtg tcatattaat tttcgtttaa tgtcacatat
      301 aattggtgta taatttttgc agtttaaaga agcttttgga gatagaaatg gaaccttatc
      361 aacgggaagc aatttctgta gatgtatctg ttcgattgcg tgagttacgc gaggcgcgtg
      421 gcatctcaat gcgtaccctg gcttctcgaa gcgggctttc tgcaaatgca ttgtccatga
      481 tcgagcgggg gaagacctca ccatctgtga gcactttgta taagttagcg aatgcattgg
      541 gtgtttcaat tactgcgttt ttcgataccg agaccgaaaa gagacaagtt gtttttctga
      601 agagcgatgg acgcacccgt ttatctttca ctcggggcgt attcgaagcc cttggcggcg
      661 aaaattttgt tgggcgggtg gagcctttta tgctgacgat ggaaagtggc gcttcctgtg
      721 gaccgcatga cattgtccat tcggggcatg aattcgtttt ttgtctgcgt ggtcaattag
      781 attacacggt ggaaaaagag accttccgcc tcgaaccggg ggatagcttg ctcttcgctt
      841 ccaagcttcg ccatcgctgg aagaatcctg ctaatgtcgt tgcgaatgca ttgatcatta
      901 tctctggctt tgctgagggc gaagagcctc actctatgca ttggaataaa taaattaaga
      961 tgttaaataa aaagagccac ctgtacaggt ggctcttttt gctcttttac taaaactacg
     1021 cgtacatacc ccagtcaagt gcagaggggt cttccatcat ggagaagtcc cacacatcca
     1081 tgcccatttc tacagtgacg ggcatgttca agccttcagt ggccttggct ttggtcattt
     1141 cgatcatagc aggaccatac gggcagaagg gcgtggtcag gatcattttg aggcgcgcca
     1201 taccgttttc cacttcgata tcacgtacca agccaagttg gatgatgttc ataccaattt
     1261 cagggtccac tacttcagac aaactagcgc gcgcctgctt gaccaggtct gggttggtgg
     1321 tgtggatggt ccactggatt tcgttaattt gagaatcgct cataaagtcc tctttttatt
     1381 ttgttttacc gacgggttga accacatacg tacaatgagc accgccatct aaaatgcagc
     1441 gaactttgtt tgcaggcagt gccagcattt tggagatcag agtttgatct accgtgcaga
     1501 cttctgggtg tgccacgccg atctgatagt atgggcagga gatttcgtgg atctggtatt
     1561 gtccatcttt cttttcccat tccacagtaa aaccttcctg cgccaacata tccttgacga
     1621 agtcaaggcg ttcttccata cttagacctt tgatatcgtt ggcatattca ttggctaggt
     1681 cttcagcgat ctgaccgaac aacttgctca ccatgggtcc ggggatgctt tctttcatct
     1741 gagcaatgag gcgggttgtc agacgcaggt agcgggtagg gaagcgttcc atgccttcat
     1801 cggtcagtac atagaccaaa cgtgggcgtc ctaccccgtg gcgttcctct tgcgattcaa
     1861 ccagcccttc catttgcagg ttggtgaggt gatgccgcac agagatcggg ttgatgccga
     1921 ctgcatctgc cagatcattg atggtagatt tgggtttctt aagtaaagtt tgtaaaatct
     1981 tgtctcgcgt agatttcatg cagggcagta taacttccct gtaggctctt gtcaatattc
     2041 tagataaata tcataaaaat tacgagaggg ctattttgtg gtgaatagga aggtttgaga
     2101 ccaggcgctc caattcccag cactgtctat gccccgaact ctccaatagt aagttctagc
     2161 tggcaggctt tttgtttgta ttgttgcctt gttggtcgtt gcgtcgaatt cagggcttga
     2221 aaaatcggag ttgttgtcca cttgaatttg atatttcaca acgctgttta ctacagacca
     2281 tttcaaccat acccgtttgg ctgtggttgc gctagaggct gggctaagaa ggttgggggc
     2341 agcgggcgga gttgcatcta ctgcaaagtt gattgctttg ctgaatttcc cggggtttag
     2401 gtctgggttg taagcgcgga ctcgtacata atagccgcca tctgcaagtg gagtaggcgg
     2461 agttatggat gtagaggttg ttgtttgaat aaaaacaatt tgtgaaaaat tactatcgcg
     2521 tgcaacgaat acttcatatg cctgtgcatt acgcaccgca gaccatgata tagagactaa
     2581 tgggttgttg gtcaatttgc cttgaacgga attcatggtg ggtgccgaaa aatccacagg
     2641 ggttttggca atattgaaac tctcgcctgt gaaattccta ttcaggagtg ggtttccggc
     2701 tttgtctgta atgttgccgt tgtctaataa gtcgagccga atgcttcccg aaccgatccc
     2761 tgtactgacc ttgacccaat aaaatgggtc cgcattttgc atgcccacca cactcgattg
     2821 aatgacattg gaactggtca aaataaagtc acttgtttcc acgccgttca cgggctccga
     2881 aaatgtgacg atgaaatctg ccgttgggtt gatagcggga ttaccacctg cgcgaatgat
     2941 tgatgtcact tgcggcgata tacggtcaat gagataagtt tcacccgttg tgaaatttgc
     3001 attccctatc ccgttcccgc cgagtggaat gccttgggca tta
//
LOCUS       DAOREF010000326         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21538_length_3036_cov_4.385106, whole genome shotgun sequence.
ACCESSION   DAOREF010000326 DAOREF010000000
VERSION     DAOREF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21538_length_3036_cov_4.385106"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="aroB"
                     /locus_tag="PKI33_07785"
     CDS             complement(<1..1334)
                     /gene="aroB"
                     /locus_tag="PKI33_07785"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNM36946.1"
                     /translation="MKHIFLYGPSGSGKSTIGKQLANDLRLPFVDSDQIIEINAGMSI
                     PEIVEADGMPKVRDLETEALKQIIKNGKDSIVALGGGALLRDENRTLVEQNGKVLLLK
                     AEISTLMNRLDKDPNKRPLLAGDLEAKLKAYLDGRAWHYASFPLQVSVDNESVEAISK
                     QAQVLAGRHHLSAMGEYDVFVGSVSNVPNLQNPIIVTDENVAKYHLEKIQNLFNAKAV
                     VVPAGEEHKNLETVSHLWKAFLENGLDRKSTVIALGGGVIGDMTGFAAATYMRGIDWI
                     GIPTTLLSMVDASLGGKTGFDLPEGKNLIGSFHPPKLVIADPSLLLTLTERELRSGMA
                     EVVKHGIISAPDLFAMCQRGMDWVKANLEEVVKHAMAVKIKVIEEDPYEKGFRAALNL
                     GHTVGHAVELVSKFELRHGEAIAIGAAAEARYAARVGLASQSTVEAIESTLSA"
     gene            complement(1375..2547)
                     /gene="aroC"
                     /locus_tag="PKI33_07790"
     CDS             complement(1375..2547)
                     /gene="aroC"
                     /locus_tag="PKI33_07790"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNM36947.1"
                     /translation="MLRFLTAGESHGPSLTTILDGIPAGLPLSTDIINKELARRQQGY
                     GSGGRMKIEKDTVQILGGVMGGETTGAPIALLVQNDDHIKWKGKAIEPMTAPRPGHAD
                     LTGAVKYGYKDLRPALERASARETTMRVAAGAVCKHFLAQFGIIIGGYVASIGEVTAD
                     FGDMPYEERFIRAEESDVRCPIDSSADKMRAEIEKTIHGKNTLGGVLEIIALNLPVGL
                     GSFVQWDKRLEAKLAHAIMSVQAIKGVEVGDAFENAKRIGTEAHDPIQLPITNHQLLR
                     SSNRAGGTEGGVSNGQPIVIRAAMKPIATTLVPQQTVDLAKGENAPTKYERSDFCPVP
                     RAVPILESMAAFVLADALLEKLGGDSINEMKLRFESLRKATLEDLPMDNIPHVFWE"
     gene            complement(2602..>3036)
                     /locus_tag="PKI33_07795"
     CDS             complement(2602..>3036)
                     /locus_tag="PKI33_07795"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36948.1"
                     /translation="SARAVVYALANDGWNVTLAARRVEQAQQLASSIDNYQLSIVNYS
                     NLQPSTFDLLVNTTPLGMTPNTEASPLPEGTELSKNTFVYDLVYNPRETKLVRDARAQ
                     GLNASTGLGMLIEQAALAFEKWTGHNPPREILFEAVRATRPD"
BASE COUNT          700 a          748 c          808 g          780 t
ORIGIN      
        1 gctgacaatg tagattcaat cgcctcgacc gtgctttggc ttgccagccc aactcgagcc
       61 gcatacctcg cctcagccgc agcgccgatg gcaatcgcct cgccatgccg cagttcaaat
      121 ttgctgacga gttccactgc atgcccaacg gtgtgcccca aattcaacgc tgcacggaat
      181 cctttttcat acgggtcttc ttcgatgact ttgatcttca ctgccatcgc atgtttgacg
      241 acctcttcga gatttgcctt cacccagtcc atgccgcgct ggcacatggc gaacagatcg
      301 ggtgctgaga tgattccatg cttcaccact tctgccatgc cggagcgcag ttcacgctct
      361 gtcagcgtaa gcaaaaggct tgggtctgca atgaccagct tcggcggatg aaatgagccg
      421 atgaggtttt ttccttcggg caagtcaaat ccagtcttgc cacccagcga agcatccacc
      481 atggaaagca gagtagtggg aatgccgatc caatcaatac cgcgcatgta ggttgcagcg
      541 gcgaagcctg tcatgtcacc gatcacgccg ccaccgaggg caatgacggt gcttttgcgg
      601 tcgagcccgt tttcgagaaa ggctttccac aagtgcgaaa cagtttcgag gtttttatgt
      661 tcttcgcccg caggcacaac tactgctttg gcgttgaaaa gattttgaat tttttcgaga
      721 tgatatttcg ccacgttctc atctgtgacg ataatggggt tttgcaggtt gggaacattg
      781 gatacagaac ccacaaagac atcatattca cccatcgcag agagatgatg ccgacctgcc
      841 aacacctgtg cctgctttga aatcgcttca acgctttcgt tgtcaaccga aacttgcaat
      901 gggaaggaag catagtgcca agcacgacca tcaaggtagg ctttgagttt tgcttcaaga
      961 tcaccagcca atagcggacg tttattcggg tctttatcaa gcctgttcat gagagtggag
     1021 atttctgctt ttagaagaag tactttgcca ttctgctcga caagtgtgcg gttttcatct
     1081 cgcaacaaag cccccccacc aagcgcaact atgctatctt tgccattctt gatgatctgc
     1141 ttcaaggctt cggtctcaag gtcacgcact ttaggcatgc cgtccgcctc gacgatctca
     1201 ggaatggaca tacctgcatt aatctcgata atttgatcag aatcaacaaa gggaagtctc
     1261 aaatcgttag caagttgctt accgatcgtt gatttacctg aaccggaagg tccgtatagg
     1321 aagatatgtt tcataatgaa gaatacagaa caatgattga tgagagatac atgtttactc
     1381 ccaaaacacg tgcgggatgt tatccatcgg taaatcttcg agagtggctt tacgcaatga
     1441 ctcaaagcgc agtttcattt cgttgatgga gtcaccgcca agtttttcga gcagggcatc
     1501 ggcaaggacg aaagcggcca ttgattctaa aattggtaca gcgcgtggaa cgggacaaaa
     1561 atcggagcgt tcgtatttgg tgggagcatt ctcgcctttg gcgaggtcca cagtctgttg
     1621 cgggacaagg gttgtggcta tcggtttcat cgcggcgcgg atgacgatgg gctgaccgtt
     1681 gctcacaccg ccttcggtgc cgcctgcgcg gttggaagag cgaagtaatt gatgattggt
     1741 gattggtaat tggattgggt cgtgggcttc ggttccgatt cgttttgcat tttcaaaggc
     1801 atcgcccact tccacgcctt tgatggcctg gacagacatg atggcgtggg caagtttggc
     1861 ttcgaggcgt ttatcccatt gcacgaaact tcccaaacca acgggcaaat tgagtgcgat
     1921 gatttcgagc acgccgccga gcgtgttctt gccgtggatg gtcttttcga tttcggcgcg
     1981 cattttgtca gcggaggaat cgatcgggca acgcacatcc gattcttcgg cgcggataaa
     2041 gcgttcttcg taaggcatgt cgccaaagtc tgcggtcact tcaccgatgg aggcgacata
     2101 tccgccgata atgattccaa attgagcgag gaaatgtttg cagaccgcac ccgccgcaac
     2161 acgcatggta gtctcacgag cggaagcacg ttccaatgca ggtcgcagat ctttgtagcc
     2221 gtacttgacc gcgcctgtta aatccgcgtg acccgggcgg ggagcggtca tcggctcgat
     2281 ggctttgcct ttccatttga tatggtcatc gttttggacg agcagggcaa tcggcgcacc
     2341 tgtggtttcg ccgcccatca cgccgccaag aatttgcacg gtatcttttt ctattttcat
     2401 gcgtccgcct gagccatagc cttgctggcg gcgggcgagt tctttgttga taatgtcagt
     2461 agataaaggc aaaccagcag ggataccatc caaaattgta gtcaacgatg gtccatgtga
     2521 ttcaccagca gttaaaaatc taagcatacg ttctccattc ataaagcagg ggcgaccctt
     2581 tgtggtcgtc cggagttttg atcaatcagg gcgggtcgcc cttacagctt caaataaaat
     2641 ttcacgcggc gggttgtgcc ctgtccattt ctcaaaggcg agggcggctt gttcgatgag
     2701 catgcccaga ccagtggagg cgttcaatcc ctgtgcgcgg gcgtcgcgga caagtttggt
     2761 ctcgcgtgga ttgtagacca gatcataaac aaaagtattt tttgataatt cagtaccttc
     2821 gggcaagggg gaggcttcgg tattgggcgt catgccgagc ggggtggtgt tgacgagcag
     2881 gtcgaaggtt gatggttgaa ggtttgaata attgacaatt gataattgat aattatcaat
     2941 tgaacttgcg agttgttggg cttgttcgac gcgacgggcg gcaagggtga cattccagcc
     3001 atcattggct aaagcataaa ctacggctcg cgcaga
//
LOCUS       DAOREF010000327         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21562_length_3034_cov_4.924807, whole genome shotgun sequence.
ACCESSION   DAOREF010000327 DAOREF010000000
VERSION     DAOREF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21562_length_3034_cov_4.924807"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKI33_07800"
     CDS             <1..745
                     /locus_tag="PKI33_07800"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36949.1"
                     /translation="TYALSRFIQSPGLTLSLSLPGFYYSFPLTLSFFLSMLAAALTAA
                     GMDWLTRDHPSAVSRATREHLLLPTLTAFVLGTPLALLSNSSAWWWAFLVGAVLLAAV
                     CIAEYISVDPTAPLYGFARAGLTAVAYALFLILVTSLRFSGARMVLVIPSVLIVAGLI
                     SLRILHLDGTDRWDFPWAVGIGLVCAQIGAGLHYWPLSPVQFGLAVTGPLYALTMLSN
                     SLTENIPLRRAVVGPIIVAGVAWVTAIFL"
     gene            748..1164
                     /locus_tag="PKI33_07805"
     CDS             748..1164
                     /locus_tag="PKI33_07805"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNM36950.1"
                     /translation="MRTLSLTNEQLQQMIAHVDSQAPLEACGLLAGRGSSVESVLTVT
                     NQAQSPVRYVMDPIEQLHAFEWIESQGLELLGIFHSHPAGPEVVSPTDIAEAAYDVVY
                     VIVSRINSPWKARGFWIQDGKFDEVTLQIVSSSNQP"
     gene            1234..1920
                     /locus_tag="PKI33_07810"
     CDS             1234..1920
                     /locus_tag="PKI33_07810"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNM36951.1"
                     /translation="MQIVLAILAVVLAYVIGSVPVGLLIVKIATGKDIREVESGRTGG
                     TNAMRAAGFWAGFATAMLDIVKGAAGVWVARWLTPDLPVVHMIAPLAAILGHNHSIFL
                     PERDENGRIIRLRGGAGGAPSVGGAMGLYLPSILIVLPLGMLTFFSIGIASVTTMAVA
                     LYSTLAFAYYASQGIIPWAYVLYGVGAEILLVLALRPNIKRLFEGNERVVKYSLNGWL
                     RKRKEEKAAK"
     gene            2020..2598
                     /locus_tag="PKI33_07815"
     CDS             2020..2598
                     /locus_tag="PKI33_07815"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNM36952.1"
                     /translation="MPTREKILDTALTLFNNEGTAAVSTNHIAEAASISPGNLYYHFH
                     NKEEIIRGLFERLFVANDAAFRLSEDKLPTLNDLQTMVKTNYKILWQYRFAHRELAAL
                     LRADEELRTRFLSVRKRGFEGFGQLFQAFTQAGVFKTQSTPEEIQNLAEVCWLITEFW
                     LNSLEVSGKTVNETQMSHGVELMMQVLKPFIK"
     gene            2608..>3034
                     /locus_tag="PKI33_07820"
     CDS             2608..>3034
                     /locus_tag="PKI33_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNM36953.1"
                     /translation="MSQNNFRKLAGLFFIVGAVLVNIPYTLLIMNFDYPDILRLPTAE
                     ILTRFQAGGNALIYTWLAFAWVGLPMLFGAIMLKRILADEQAPFLETATTLGVIGFIV
                     QVVGLLRWVFVVPALTRLYTDPSTDSVTQAAVAAVFTAVH"
BASE COUNT          657 a          784 c          749 g          844 t
ORIGIN      
        1 gacatacgcc ctctctcgtt tcatccaatc tccgggactc acgctcagcc tgagcttgcc
       61 cggtttctac tattcttttc cactcacact cagtttcttc ctctcaatgc ttgccgcggc
      121 tcttaccgcc gccggaatgg attggctcac ccgcgaccat ccctccgctg tgagtcgcgc
      181 tactcgcgag catttgctgt tgcccaccct caccgcattt gtattaggaa cccctctcgc
      241 ccttctctcc aatagttctg cttggtggtg ggcattcttg gttggtgcag tgttgctggc
      301 agccgtttgt attgccgaat atatttccgt cgacccaacc gctcctttgt atgggtttgc
      361 gcgtgccggt cttactgctg ttgcctatgc cttgttctta atccttgtca cttcgttaag
      421 attttccggt gcgcgcatgg tgctggtgat tccatcagtg ctcatcgttg ccgggttgat
      481 cagtttacgc attctgcacc tcgatggcac agaccgttgg gattttccat gggcggtagg
      541 aattggtttg gtctgcgcgc aaattggcgc cgggctgcat tactggccgc tttcgccggt
      601 tcaattcggg ctggctgtga ccggtccgct atatgcgttg accatgctct cgaacagcct
      661 tacagaaaat atcccattgc gccgcgcggt agtgggaccc atcatcgtgg caggcgttgc
      721 ttgggtcact gcgatctttt tgtaagcatg cgcactcttt cccttaccaa cgaacaactt
      781 caacagatga tcgctcatgt cgattcgcaa gctccactgg aagcttgtgg tctattagcg
      841 ggtcgcggct ccagcgtgga gtctgtgttg acggtgacga atcaggcgca aagtccggtg
      901 cgatacgtga tggatcccat tgagcaattg catgccttcg agtggatcga gtctcaaggg
      961 ctagaactgc tcggcatctt ccattcacac ccagccggcc ccgaagtggt ttctcctact
     1021 gatattgctg aggctgctta tgatgtggtg tatgtaatcg tttcccgcat taacagccca
     1081 tggaaagcgc gcggattttg gattcaagac ggcaagtttg atgaagtgac cttgcagatt
     1141 gtctcatcta gcaaccaacc ctaaggtttc gtgtttttac gtctgctaat ttgtgatgtt
     1201 aaattttaaa cagcaactcg tggaggagtc ttaatgcaaa tcgtcttagc cattctggca
     1261 gttgtactgg cttatgtgat cgggtcggtt ccggtggggt tactgatcgt caagatcgct
     1321 accggaaaag atattcgcga agtggaaagc gggcgcaccg gcggtacgaa cgccatgcgt
     1381 gcagcagggt tttgggctgg gttcgctact gccatgctcg acattgtgaa aggcgcagca
     1441 ggggtttggg tggcacgttg gctcaccccc gatctgcccg ttgttcacat gatcgctccc
     1501 ttggcggcaa ttctcggaca taaccattcg atctttctgc ccgaacgcga tgagaatgga
     1561 cggatcattc gtttacgtgg cggtgcaggc ggcgctccat ctgtaggtgg cgcaatgggc
     1621 ttgtatcttc catctatttt gatcgtcctg ccgcttggca tgttgacctt ctttagtatt
     1681 gggattgctt ctgttacaac catggcggta gcgttgtatt ccactttggc atttgcttat
     1741 tatgcttcac aaggcatcat cccttgggca tatgtccttt atggggtagg agcagagatt
     1801 ctgttggtgt tggcattacg cccgaatatc aaacgtctct ttgagggcaa tgaacgggtt
     1861 gtaaaataca gcctcaacgg ctggctgaga aaacgtaaag aagaaaaagc agcgaagtaa
     1921 gctaaaacgc cgaggtcttg aagacctcgg cgttttttta cccttgacag tctagagtat
     1981 ttactctaaa atattagagt aaatactcta ggagatatta tgcccactcg tgaaaagatt
     2041 ttagataccg ccctcaccct cttcaataac gaagggactg ctgccgtcag caccaaccac
     2101 atcgccgagg cggcaagcat cagtccgggg aatctctact atcacttcca caataaggaa
     2161 gagattattc gaggtctatt cgagcgtcta ttcgtagcga atgatgcggc atttcgtttg
     2221 tcggaagata aattgcccac gcttaatgac ctgcaaacga tggtcaagac caattacaag
     2281 attctctggc aatatcgttt tgcacaccgc gaacttgccg cactcttgcg agcagatgaa
     2341 gaactacgca cgcgctttct ctctgttcgt aaacgtgggt ttgaagggtt tggtcagctc
     2401 tttcaagcct ttacccaagc tggcgttttc aaaacacagt caaccccaga agaaattcaa
     2461 aaccttgccg aggtctgctg gctcatcact gaattttggc tgaacagcct tgaagtaagc
     2521 ggcaagaccg tcaacgaaac gcaaatgtct catggcgttg agctaatgat gcaagtatta
     2581 aaaccattta tcaaatagga aaaaatcatg tcacaaaata attttagaaa actagccggt
     2641 ctctttttta tcgtgggtgc agtgttggtc aatatcccct acacgctgct cattatgaat
     2701 ttcgattacc ccgatattct gcgcctaccc acagcagaaa ttctcacacg gtttcaagca
     2761 ggcgggaatg ccctcatcta cacttggctt gctttcgcat gggtgggttt accaatgctc
     2821 tttggcgcga tcatgctcaa acgaatctta gctgatgaac aagctccatt tctggaaaca
     2881 gcgacaacgc tcggcgtgat cggcttcatc gtccaagtgg tgggactctt acgctgggtc
     2941 ttcgttgtgc ctgctctcac tcgcctctac acggacccgt caacggattc agtcacccaa
     3001 gccgcagtcg ccgctgtatt cactgccgtc cacc
//
LOCUS       DAOREF010000328         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21681_length_3026_cov_3.771794, whole genome shotgun sequence.
ACCESSION   DAOREF010000328 DAOREF010000000
VERSION     DAOREF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21681_length_3026_cov_3.771794"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..860)
                     /locus_tag="PKI33_07825"
     CDS             complement(126..860)
                     /locus_tag="PKI33_07825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM36954.1"
                     /translation="MDLREYELMYQVEDSHWWYEGMSTITCSLLDYWVSAKNLRILDA
                     GCGTGAAMTRYLSDYGSVTGIDLYAEALHFCLKRNAKRLARASVLKLPFASNSFDLIT
                     SFDVLYERAVLDETTALKEFSRILAPNGHVLLRLPAYDWLRGKHDERVHTNRRYTTRQ
                     VAGLLEKSGLHVQHLSYANTILFPLAVAKRLSERFFPPSEDYSELSLNAGMFNNFLKF
                     ILASEALFITKYGLPFGLSVIAVGRK"
     gene            complement(873..2837)
                     /locus_tag="PKI33_07830"
     CDS             complement(873..2837)
                     /locus_tag="PKI33_07830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM36955.1"
                     /translation="MTERSSKTSGDVNNIRRAIAFVGIMCILISQFLIFSQPVDHTIV
                     FPPYTFLAILGVIIFSFSWFIKPSPILERAASWLLFQDRAFWLFVAFALSGVVTFAIV
                     NLTLFARINYIPVVTLWLFSASAYVYVFWDRSINLSTLVDWFKKNQNELLSVLAVTLL
                     AVVVRFYKLGGIPRILDGDEGLIGLFAQSTTSGTLANPFALWENFGAMYLQLINTSIG
                     LFGVNAFALRLLPAIGGTLAIPSIYLLARWIGGRRIALIAALILAFSHTHIHFSRIVS
                     VAYIHGTWLAPLELYLLLSGLEKRQSWRTALSGIILAIHFSVYVTAQVIAALAFIFML
                     LSLIFYRQWFKERIRQALVFWGAFLTLILPSAYYIFKNPNEFFSRMSANGTFQSGWLQ
                     LTMETTGQSAVEVLFGRFVHAFLSLIYYPAFDFYGSTSPVMSMITSAFFLAGLVIALL
                     RLRKSEYLLLNGYFWGATFSVGIFAIPPSADSYRMLMALPAAFIMAALGLDQILEAFG
                     FGWKNNRSAYIFSASAVLTSLLIFNMWTYYGDFAGKCRFGNDLVGRFASYLGSYAGSV
                     ENEYPIYLLSDTNYFYGSHGSTDFLSQRRAIINVSDPVDTLNPVSGETIIANPDRIPE
                     LEAWARTHPGGQLNYRYDCDKTILLAYQVP"
BASE COUNT         1017 a          688 c          601 g          720 t
ORIGIN      
        1 gacgacaatg atctcaaaat cattcgccac ctgcttgcaa gcgatactag ctgcagccac
       61 catgcttgcg attgttccgc catcgttata tgctgggaaa acagtactta tactggaaat
      121 tttgctcatt ttcgccccac tgcaatgacg ctcaagccaa atggcaagcc gtatttcgta
      181 atgaacagcg cctcgctagc aagaataaat ttcaaaaaat tattgaacat cccagcattc
      241 aaagaaagct cagaataatc ttcactggga ggaaaaaacc tctcgctgag tctttttgct
      301 acagcgagag gaaataggat tgtatttgca tatgaaagat gttgcacatg cagtccgctc
      361 ttttcgagca agccagccac ttgtctggtt gtataacgac ggttggtatg aacccgttca
      421 tcatgctttc ccctaagcca atcataggca ggcaagcgca agaggacgtg tccattcggg
      481 gcaaggatac gagaaaattc ttttaaggct gtcgtttcat ccaagacagc ccgttcgtat
      541 aagacgtcaa agctggtaat taggtcaaag gaattagagg caaacggcag cttaagcaca
      601 gaagcgcgcg caagccgttt tgcattccgt ttcagacaaa agtgtagagc ttctgcgtac
      661 aggtcaattc cagttactga cccataatca gaaagataac gggtcatggc agcccctgtg
      721 ccacaaccag cgtctaatat tcttaagttt ttagccgata cccaataatc aagtaaagag
      781 caagtaatgg tactcattcc ttcataccac caatggctgt cttccacttg atacatcagc
      841 tcatattcgc gtaaatccaa atctcctctt tcctaaggga cttgatatgc caataaaatg
      901 gttttgtcac aatcatagcg atagttcaat tgtccgccag gatgagtacg agcccaagcc
      961 tccagttcag gaatacgatc tggattggca ataatggtct caccagaaac gggattcagg
     1021 gtatcgaccg ggtcagatac attgataatc gctcggcgct gacttaggaa atcggtagaa
     1081 ccgtggcttc cataaaaata gtttgtatca ctcaaaagat aaattgggta ttcattttcc
     1141 acacttcctg cataactacc taagtaagaa gcaaaacgcc caacaaggtc attaccaaag
     1201 cgacattttc cagcaaagtc tccgtaatac gtccacatgt taaagataag caagctggta
     1261 agaacagcag atgctgaaaa gatataagct gagcgattat tcttccaacc aaaaccaaat
     1321 gcttcaagga tctggtctag ccctaatgca gccataataa aagcggcagg aagcgccatt
     1381 aacattcggt atgagtctgc gctgggggga atcgcaaaaa tgcctaccga gaatgtagcc
     1441 ccccagaaat atccgttcaa aagcaggtat tccgatttcc gaagtcgtaa gagggcaatc
     1501 accaagccag ccagaaaaaa agcactggtt atcatgctca ttaccggtga ggtagaacca
     1561 taaaaatcga aagctggata atatattaaa gacaagaagg catggacaaa ccttccgaat
     1621 aatacttcaa cagcactttg tcctgtcgtt tccatggtaa gttgaagcca gccagattga
     1681 aatgtcccat ttgcagacat gcggctaaag aactcattcg ggtttttgaa aatatagtac
     1741 gccgatggca gaataagagt caagaaagca ccccaaaata ccaatgcttg tctgatgcgt
     1801 tccttaaacc attgccgata aaatatcaac gacaacagca taaaaataaa tgcaagtgca
     1861 gcaatcactt gtgcagtaac ataaaccgag aagtgtattg caaggatgat cccgctcaat
     1921 gcagtacgcc atgattggcg tttttctaag ccactcagaa gtaaatacaa ttccagtgga
     1981 gcaagccatg taccatgaat gtaagccaca gaaactattc tgctgaaatg aatatgtgta
     2041 tgagaaaagg cgaggatcaa cgcagcaatc aaagcaatcc ttcgcccgcc aatccagcga
     2101 gccaaaagat agatagacgg gattgccaga gtaccgccaa tagctggtaa aaggcgtaga
     2161 gcaaaagcat ttactccaaa caaaccaatg gaagtattaa ttaactgcaa atacattgcc
     2221 ccaaagtttt cccaaagtgc aaaggggttc gccaatgttc cactggttgt agattgagcg
     2281 aaaagaccaa tcaacccctc gtctccgtct aaaatacgtg gaataccacc taacttataa
     2341 aacctgacaa caactgccag caaagtaacc gcaagaaccg agaggagttc attctgattt
     2401 ttcttaaacc aatcaacaag tgttgataag tttatgctac ggtcccagaa aacatataca
     2461 tatgcactag cactaaatag ccaaagggta actaccggaa tgtaattaat acgtgcaaac
     2521 aaagttaaat tgacaatcgc aaaagtcacg acgccagaca aggcaaaggc aacgaacagc
     2581 caaaatgccc ggtcctgaaa cagaagccag gatgcagccc tctccaaaat gggactgggt
     2641 ttaatgaacc aactaaaaga aaaaataatg acgccaagaa tggctaaaaa tgtgtacggc
     2701 ggaaatacaa ttgtatgatc taccggttga gaaaaaatca aaaactgact gattaagata
     2761 cacatgatcc ccacaaaggc aattgccctt cgaatattgt ttacatcccc gctcgtctta
     2821 ctgcttcgtt cagtcatcac atcctcctaa gatgaattat cttactgacc tacacataat
     2881 cgacatcaat gcgaaaggac tttcattata cccaaataaa gaagatgtct agctcagtcc
     2941 ttatcatgaa tttatcaggt taacaattct taaaattcat gtataattaa gaaaacttaa
     3001 gacaccttcc gctttgcggt cttttg
//
LOCUS       DAOREF010000329         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21779_length_3017_cov_3.756921, whole genome shotgun sequence.
ACCESSION   DAOREF010000329 DAOREF010000000
VERSION     DAOREF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21779_length_3017_cov_3.756921"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..856)
                     /locus_tag="PKI33_07835"
     CDS             complement(47..856)
                     /locus_tag="PKI33_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNM36956.1"
                     /translation="MNAYNEYVNTARKKREDSFASNPQSWLNLVGLFWLEEGKNSFGR
                     SPEAKISLPQFPQDVCGYFLFKNGLVTVHPQTTMTMNNGAIESRPLWTDKDPQADTIE
                     LGTLIMKIIVRGDATLVRVWDKEAELKKGFKNFNYYTPDPKFKVTAKYIRYNPPKPTI
                     RIEGIGTEVKTFFMGQALFTLNGKEYTLQAEESGDELLFNFKDETNADTTYGGGRRFY
                     LPLPDSDEIILDFNLTDNWPCAYTPFATCPIPPKENVLKVRIEAGEKIFKK"
     gene            complement(859..1737)
                     /locus_tag="PKI33_07840"
     CDS             complement(859..1737)
                     /locus_tag="PKI33_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNM36957.1"
                     /translation="MKFKPWQIILAVLALILLGLRITSKPAPNHPYYSDNLDYPLVIA
                     HQGGDGVWPGETMFAYQNSADLGVDVLEMDIHITKDGELILMHDETVDRTTDGSGEIE
                     SMTLDELKKLDAAYDWTPDDGQTFPYRGKGIQIATLAEVFQAFPDKHMTIEIKKTNTS
                     MAKPFCDLIRKNNMQDKVLVASFHDDRLKEFRAECPEVATSSAKNETTVFVLLTKVYL
                     GGLFSPKFYSLQVPEVSGGITVMTPAFVRAAHARNLAVEPWTINDAETMRKLIDWGVD
                     GIITDRPDIMMEVLGR"
     gene            1841..2278
                     /locus_tag="PKI33_07845"
     CDS             1841..2278
                     /locus_tag="PKI33_07845"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNM36958.1"
                     /translation="MLALRLLAEGGEGLNTELLWLLWSILGLFVLAIIAGWLSSLRMP
                     KQVTASHEADSSSANEKVHDDLSKIEGIGPKVVKVLARAGITTFEELATANVDDVQNT
                     LNKAGLQMLNPEGWIDQAKLAVQGDWDGFEKLQKKLKGGRKKK"
     gene            2398..2793
                     /locus_tag="PKI33_07850"
     CDS             2398..2793
                     /locus_tag="PKI33_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNM36959.1"
                     /translation="MKKVFFSLLVLSAIVLAACGNSANATAIPTVPAEYAGATNPLGP
                     DAATAGAEVFKTNCESCHGPQGHGDGPAGAALDPQPKNLAEFAPQVGDDYLYWRVNTG
                     KEGTAMVAWKGVLTDEQIWQAVAFVRTLK"
BASE COUNT          755 a          614 c          745 g          903 t
ORIGIN      
        1 tagataaata acaaaaaagc gagtggcttg ccactcgctt ttttgtttac tttttgaaaa
       61 tcttttcacc agcctcgata cgaaccttca gtacattttc ttttggtgga atcgggcaag
      121 ttgcaaatgg agtgtaggca cagggccagt tgtcggttag gttgaaatct aaaataattt
      181 catcactatc tggcagtgga aggtaaaacc tacgtccgcc gccataagtg gtatctgcat
      241 tggtttcatc cttgaaattg aagagcagct cgtctccgct ttcttcggct tgaagtgtat
      301 attctttccc attcaaagtg aaaagggctt gacccatgaa gaaagttttt acttcggtac
      361 caatgccttc tattcgaatt gtgggtttgg gcgggttgta acgaatatat ttggcagtga
      421 ctttgaattt tgggtctggt gtgtagtagt taaagttttt gaagcctttc ttgagttctg
      481 cttccttatc ccatactcgc acaagggtcg cgtctccgcg cacgataatt ttcatgataa
      541 gcgtgcctag ttcaatggtg tcggcttgcg ggtctttgtc tgtccataat ggacgagact
      601 ctatcgcacc attgttcata gtcattgttg tttgtggatg tactgtgaca agcccatttt
      661 tgaagaggaa gtatccacaa acatcttgag ggaattgtgg aagggaaatc ttcgcctcag
      721 gactacgccc gaaactgttt ttgccttctt ccagccaaaa caagccgaca aggtttaacc
      781 aactctgtgg attcgacgca aaactatctt cccgcttttt gcgagcggtg ttgacatatt
      841 cgttatatgc attcatgttt atctgcctaa gacttccatc ataatatctg gacggtctgt
      901 gatgataccg tccacacccc aatcaatgag cttacgcatt gtctctgcgt cattgatcgt
      961 ccacggttct acggcgagat tgcgcgcatg tgccgcacgg acaaaagctg gagtcattac
     1021 tgtgattcca cccgatactt cagggacttg cagagagtag aactttggtg aaaaaagccc
     1081 gcctaaatat acttttgtga gcagaacaaa tacggtggtt tcattttttg cacttgaggt
     1141 ggcaacttct gggcattcgg cacgaaattc tttgaggcga tcgtcatgaa aagatgccac
     1201 aagaactttg tcttgcatat tgtttttgcg aatcaagtca caaaagggtt ttgccataga
     1261 ggtgttggtt ttcttgatct caattgtcat gtgcttgtct ggaaaggctt gaaaaacctc
     1321 tgcaagagta gcaatctgga tgcctttccc acgatagggg aaggtttgtc catcatcggg
     1381 agtccaatca taggcggcgt cgagcttttt caattcatcg agggtcatag actcaatttc
     1441 accgctcccg tcggtggtac ggtcaacggt ttcatcgtgc atgaggatga gttcaccatc
     1501 tttagtgatg tgaatgtcca tttcaagcac gtccacgccg aggtctgcgg agttttgata
     1561 ggcaaacatt gtttcgccgg gccagactcc atctccgcct tggtgggcga tgacaagtgg
     1621 ataatccaaa ttgtctgaat aataagggtg gtttggggct ggtttggacg ttattcgtaa
     1681 accgagcaaa atgagggcta ggactgcaag aatgatctgc caaggcttga atttcatgag
     1741 ttactccaga aaagaattgg gcaattttac ccttaagaat tgacatgctc cattttttca
     1801 cgtataagtg ccaaatcaat tacatcctat ggaggaagat atgcttgcat tgcgtttgtt
     1861 agctgaaggt ggggagggat taaacacgga acttctctgg ttgttatgga gtattttagg
     1921 gctctttgtg ttggcgatca ttgcaggttg gctatccagc ttgagaatgc cgaaacaggt
     1981 tacggcttcg catgaagcgg attcttcatc cgcaaatgaa aaagttcatg atgatttgtc
     2041 taaaatcgag gggattggtc caaaggttgt gaaagttttg gcacgtgctg gtattacgac
     2101 ctttgaagaa cttgcgactg ccaatgttga tgacgttcaa aatacgctca ataaagctgg
     2161 tttgcagatg ttgaacccag aaggctggat tgaccaggcg aaactggctg tgcaagggga
     2221 ttgggatggt tttgaaaagt tacagaaaaa attaaagggt ggacgaaaga aaaagtaatg
     2281 atttgtcttg ccgtttgcta atagatgtca tgataacaat gtgacatatg tcacaatgtt
     2341 tttttcttca taatatcact ataataacag ccgataacaa tcaactggag gtatcctatg
     2401 aagaaagtct tttttagtct attggttttg agcgcgattg tgctcgcggc ttgtggtaat
     2461 agtgcgaatg cgactgctat tccgaccgtt cctgctgaat atgccggggc tacgaacccg
     2521 ctcggacctg acgctgctac tgctggcgca gaagttttca agaccaactg tgaatcctgc
     2581 catggtcctc agggtcatgg tgatggtcct gccggtgcgg ctcttgaccc tcaaccgaag
     2641 aaccttgctg aatttgcccc gcaggttggt gacgactatc tttactggcg cgttaatact
     2701 ggtaaggaag gcaccgctat ggtcgcttgg aagggtgttt tgaccgacga gcagatttgg
     2761 caggcggttg ctttcgtccg cactctcaaa taattgtttc ggtaaaaaag tccgtctcaa
     2821 ttgagacgga cttttttatt tctagctttc aagtgcttct aatatcttat cttttaattc
     2881 accggggtga aaaggcttgg tgaggtaatc tgtagcccca gcaccaaagg cgactgctcg
     2941 gctatcccca tcatctaatg cagtgacgat gataataggg gtgttaatga ataggggttc
     3001 ttctcgtaac atgcggc
//
LOCUS       DAOREF010000330         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21846_length_3012_cov_4.637809, whole genome shotgun sequence.
ACCESSION   DAOREF010000330 DAOREF010000000
VERSION     DAOREF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21846_length_3012_cov_4.637809"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PKI33_07855"
     CDS             complement(<1..563)
                     /locus_tag="PKI33_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36960.1"
                     /translation="MDGLICGLIIAGIVVISFFFYLLNTARKKGNTDKNQFGKTEYSP
                     VYVSIADKPDSIIRGMNKFVAEARKTETAGDKWRWIPMLIFFAGIGLALIDVFILLLG
                     YSISFVFIGGGVLLWIAAFVMARALRRSDLLDFSPRYNGTKEILHTLRDDLKPGSTFL
                     GHLDLTGSMLQNKVARETQDAQSRTTQL"
     gene            631..1794
                     /locus_tag="PKI33_07860"
     CDS             631..1794
                     /locus_tag="PKI33_07860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM36961.1"
                     /translation="MKNVLYTAFDIVPSPKGASTHILHNLRGLVNGNFNVHLITPNDG
                     VLPPEDTIEGARLTRISQDLSQNFLARAVHFGKSVLSHLALNPGYDVVHYRNIWDGFH
                     ITQNKKKFGYKTIFEVNGLSSIELKYHYPGMDASLLAKIKEQELATLHLSDAIICPSN
                     VTRDYIASLGLNRKLVTVIPNGVSPSDFPVTPLPIRDGREPVLLYIGTLADWQGLDIV
                     IKALPKILEQQPVKLRILGRGRSRQRKMLAKQIRKLGLDEYVLVQPAVPHHEIPAVIA
                     SADLCVAPLGLNDRNVTQGACPIKVLEYMAAGRPLLASNMPIVRELVREDVDGLLFSP
                     NDPDDLARQANQLLKDMELSKRLAESAAGYVREKFTWHESQKKLLKVYEKLIK"
     gene            1862..2866
                     /locus_tag="PKI33_07865"
     CDS             1862..2866
                     /locus_tag="PKI33_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36962.1"
                     /translation="MLDFIKEAGFPAIALLLGGVMVVIAIIRNIALEKVKIQLTSSQS
                     IQLAVVGVLFIIGGFVLQYFPPSSANTPENVDVYSTLYALQTQMVQPVNPQGQTNQTS
                     TPAGFALVSPTVTLFPNPVATLTPTLIPFTLTPDPSQPLAEVFCTNFYSIMVREGPND
                     NYPIKSVIENPNNLSNPDCMLFDFRMPDNSWIRIAANQDNPNYTQHELGWVTSDQFRP
                     IDFDKLRVYIPENVQDGLYCVTSRYGLKVRSCPNEGCREVGNLSLQDCLFMDGRSSDG
                     LWVRVSADKNDEKYASYKGNWVSTYFLAPFEFSAGYQPYFRYYFELLPTVEQIPTPNG
                     "
BASE COUNT          791 a          721 c          659 g          841 t
ORIGIN      
        1 agttgagttg tgcggctttg agcatcttgt gtttcgcggg ctactttatt ttgcaacatg
       61 gaacccgtca agtcgagatg accaaggaat gtagagccag gtttgaggtc gtctcgaagc
      121 gtgtgcagaa tttcttttgt cccgttatag cgcggagaga aatccaacaa gtcactgcgg
      181 cgtaaggcac gtgccatcac aaaggctgcg atccaaagta gaacaccgcc gccaatgaag
      241 acaaaggaga tgctataccc aagtagtaag ataaaaacat caatgagtgc cagcccaatg
      301 cccgcaaaga agatcagcat cggaatccaa cgccacttat ccccggctgt ttcggtcttt
      361 cgcgcttcag ccacaaactt gttcattccg cgaatgatcg agtctggctt gtctgcaatc
      421 gaaacataga caggtgaata ctctgttttg ccgaattgat ttttgtcggt gtttcctttt
      481 ttacgtgcgg tgttcagcag gtaaaagaaa aaagagatga caacgatccc cgcgatgata
      541 agcccacaaa tcagaccatc catccatgcc tccgagttat ttttatgccc acagtcgcaa
      601 tttgtgaccg cgctgggtat aatttcttga atgaaaaacg ttctctacac cgcattcgat
      661 atcgtgccaa gcccgaaggg tgccagcacc catattctcc acaatttgcg agggttggtc
      721 aatgggaact ttaatgtcca tcttatcacg cccaacgatg gcgtgctccc ccccgaagat
      781 actattgaag gcgccagact cacgcgcatt tcgcaagacc tttcacagaa tttccttgcc
      841 cgtgccgtgc attttggtaa gtcagtttta tcgcacctag ccctcaaccc cggctatgat
      901 gttgtccact atcgcaatat ttgggatggc tttcacatca cacagaataa aaagaagttt
      961 gggtataaaa ctatttttga ggtgaatggg ctttcttcca ttgaactcaa atatcattac
     1021 cccggtatgg atgccagcct gctcgccaag atcaaagaac aggaattggc gactctgcac
     1081 ctgagcgacg ccatcatctg cccatcaaac gtgacgcgcg attacatcgc ctcgctcggt
     1141 cttaaccgca agctggtgac tgttatcccg aacggagtca gcccttcaga ctttcccgtt
     1201 acccctttgc ccattcgaga tggacgcgag ccggttttgc tttacatcgg tacattagcc
     1261 gattggcaag gcttggatat cgtcatcaaa gccctgccga aaatccttga gcaacagcct
     1321 gtcaaactcc gcatcctcgg gcgcggacgt tcccgtcagc gtaagatgtt ggcaaagcaa
     1381 attcgcaaac tgggtttgga tgaatatgtt ttggtgcaac ctgctgtacc gcatcatgag
     1441 attccagcag tgatcgccag cgcagatctt tgtgttgcac cactcgggtt gaatgaccgc
     1501 aatgtgactc aaggtgcttg cccgatcaaa gtgcttgagt atatggcggc tgggcgacct
     1561 ttactcgcct caaatatgcc cattgtccgc gaactggtgc gcgaagatgt agacggattg
     1621 ctcttttcac ccaacgaccc agacgaccta gcgcgacagg caaatcaatt actgaaagat
     1681 atggaactat ccaaacgctt ggctgagtct gcggcgggat acgtccgcga gaaatttaca
     1741 tggcacgagt cacagaagaa attgttgaaa gtgtacgaaa aactaataaa gtaacagacc
     1801 ttcttttttg ttgaaaatct accccggttt cagtatataa ttttcgaata ggaggagcaa
     1861 catgttagat ttcattaaag aagccggttt tcccgccatt gctttgttgc tcggaggggt
     1921 aatggttgta attgctatca tcagaaacat tgcccttgaa aaagtaaaaa tacaacttac
     1981 ttcaagccag tctattcagt tggcagtggt gggtgtattg ttcattatag gtgggttcgt
     2041 tttacagtat tttcctcctt cttctgcaaa tacccctgaa aatgttgatg tttattccac
     2101 gctttatgcc ttacagaccc aaatggtgca gcctgtaaac ccacaaggac aaactaatca
     2161 gacttctaca cctgctggtt tcgctttggt ttcgccaacc gttaccttgt ttccaaaccc
     2221 agtggcgacc ctaacaccaa ccttaatccc cttcacacta actccagatc cttcacaacc
     2281 attagcagaa gtgttttgca cgaattttta tagcattatg gtacgtgaag gacctaatga
     2341 taattacccg atcaagtctg ttattgaaaa ccccaataac ttgagtaacc cagattgtat
     2401 gcttttcgat tttcgtatgc cagataactc atggatacgt attgctgcca atcaggataa
     2461 ccccaattac actcaacatg aacttggttg ggtgacttca gatcaattcc gcccgattga
     2521 ttttgataaa ttgcgagtat atatccctga aaatgtgcaa gatggtttgt attgtgtcac
     2581 cagccgatat ggtttgaaag tgcggagttg ccccaatgag ggttgtcgtg aagttggcaa
     2641 cctgtctttg caagactgtc ttttcatgga tgggcgttct tcagatggtt tatgggtgag
     2701 agtttcagcc gataaaaatg atgagaaata tgcttcttat aaaggaaatt gggtgagcac
     2761 ttatttctta gccccctttg aattttcggc tgggtatcaa ccatatttcc ggtattattt
     2821 tgaattatta cctactgttg aacaaatccc aaccccgaat ggataacgaa gaaccaggtc
     2881 acgcttcaaa cgtgacctgg ttcttttacg gcttgaatat attttctact tcggcgggaa
     2941 ttgcttttgg gcgtcctgtt ttcatatcca taaaaaccca gtcactttcg ccttttacta
     3001 aagtcttccc at
//
LOCUS       DAOREF010000331         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21888_length_3008_cov_4.250254, whole genome shotgun sequence.
ACCESSION   DAOREF010000331 DAOREF010000000
VERSION     DAOREF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21888_length_3008_cov_4.250254"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1211
                     /locus_tag="PKI33_07870"
     CDS             147..1211
                     /locus_tag="PKI33_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNM36963.1"
                     /translation="MKRGILLLIGILLLFSALIAGYILTQTYNFNGYVQPEPIKAPET
                     MLRSANGPVRLSDFQGKIVLLYFGYTFCPDVCPTSLANIKLALADLTPEEAAQVQVIF
                     VSVDPARDTPEALGQYVNMFQDNFIGATGTRDEIDWMVNAFGAKYTIESPDVNGNYNV
                     SHSAFTMVIDRNGFYVLNWAHGTPSTDMSADLHHIINKGIPVSAQILAGPTYTPVVCA
                     VTLIPSHVQNGQWLYEHHCAQCHGMDLAGNPQWQVELADGSHLAPPLNGSGSAWKYSR
                     TDLLAIIKEGRNLEKPIHMPAFKSNLADWEIDFILTYIQSTWDINQLNYQHGFMTLTP
                     NPTSTPAAPLAPLVTVTPSP"
     gene            1292..1564
                     /locus_tag="PKI33_07875"
     CDS             1292..1564
                     /locus_tag="PKI33_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36964.1"
                     /translation="MKNGTCPKCSSNEILSELPLHGGEGHPSYVDIVQPEPPNRPFIW
                     VAQSEQSKFVAYICGACGYSEFYAVRHQALNEGRKKGFKPRFDYTA"
     gene            1631..1981
                     /locus_tag="PKI33_07880"
     CDS             1631..1981
                     /locus_tag="PKI33_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36965.1"
                     /translation="MKVISIFLALINFLTGVLLILSCISANETFAWVAWKTGLGVLGV
                     TFGILTFKDSAQPVSQRKMILYGLLLVVVGVSIVAYGIHWSVISGDPKHTVMVVGSSF
                     FLHGFTSVLGMASD"
     gene            2070..>3008
                     /locus_tag="PKI33_07885"
     CDS             2070..>3008
                     /locus_tag="PKI33_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM36966.1"
                     /translation="MFTRLEKLYHQFPPRYWVIVLTFFIDSIGGTLLFPFFSLYITQK
                     FNVGMTQAGMVLAMFSIFGIVGNMIGGALTDRLGRRKLILFGLVFSALSTLSLGLVNS
                     LAVLFPLAAFIGLLSDIAGPANQAMMADILDEKQRQEGFGLMRVIRNLAWIIGPSIGG
                     FLANRSFMLLFIIDAVVSCIVAVIFFTYMPETKPQHHEEEHKEHESILKTIAGYGIVL
                     KDLAFMAFIIAAILMGAVYQQMYNSLSVYLRDNHGINPQGYGFLMTTSAITVILFQFW
                     VSRNMKHRPPFLIMAVGTLFYMLGFSMFGFVSVYWLF"
BASE COUNT          695 a          856 c          669 g          788 t
ORIGIN      
        1 ccacacaatg cttgcgccag cgcaaagaca gaacgccacg ctaaaggtaa aaattgaaac
       61 gtaggggcga ggtcccctcg ccctcgttac taagatttga ttgggcaggg aaaccccacc
      121 cctacgaaat cttgtgcgga gctcttatga aacgtggcat ccttctctta atcggcatct
      181 tgttattatt ctcagccctt atcgctggct atatcctcac ccaaacctac aacttcaacg
      241 gctatgttca gcccgaaccg atcaaagcac ctgagaccat gttacgctcc gcaaatggac
      301 cggtccgctt gagcgatttt cagggcaaga ttgtgttact gtatttcggc tacaccttct
      361 gcccagatgt ttgccccacc tcgttggcga acatcaagct ggcgctcgca gatttgaccc
      421 cggaagaagc ggctcaggtg caggtcatat tcgtttctgt agacccagct cgcgacacgc
      481 ccgaagcgct cggtcagtat gtcaacatgt ttcaggataa cttcatcggc gccaccggca
      541 cgcgtgacga gatcgattgg atggtgaatg ccttcggcgc caagtacacc atcgagtcgc
      601 ccgatgtcaa tgggaactac aacgtcagtc actcggcgtt cacaatggtg atcgaccgca
      661 acggctttta cgtgttgaat tgggcgcatg gcacaccgtc tacagatatg agcgcagacc
      721 tgcatcacat catcaacaaa ggcattccgg tcagtgcgca aattttggcg ggtccaacct
      781 atacgccagt cgtttgtgcg gtcacgttga ttccgtcgca cgtccaaaac ggtcaatggc
      841 tgtatgaaca tcattgcgcc caatgtcacg gcatggactt ggctggcaac ccacagtggc
      901 aggtagagtt ggcggatggc tcgcatctgg ctcccccgct caatggcagt ggaagcgcgt
      961 ggaagtattc tcgaaccgac ctgcttgcca tcatcaaaga aggacgcaac ctcgagaaac
     1021 ctattcacat gcctgccttc aaatcaaacc tcgccgattg ggagatcgac ttcatcctca
     1081 cctatattca aagcacttgg gatattaacc aactgaacta tcaacacgga ttcatgaccc
     1141 tcactcccaa cccaaccagc acccctgccg cgcctcttgc cccgctcgta acggtgacgc
     1201 cctctccata attttctcca ccctttggcg gatactcctt tacaaaccca caggctatga
     1261 tgagtgcatc tcatccttta ggagcttcat catgaaaaac ggaacttgcc ccaaatgtag
     1321 ttcgaacgaa atcttaagtg aacttccctt acatggcggc gaagggcatc catcatacgt
     1381 ggatatcgtc caaccagagc cgccaaaccg tcccttcatt tgggtggcgc aaagcgaaca
     1441 aagtaaattt gtggcatata tttgtggtgc ttgcggctat tctgaatttt atgccgttcg
     1501 ccatcaagct ctaaacgaag gtcgaaaaaa aggcttcaaa ccgcgcttcg attacactgc
     1561 ctgacagact ctcaatagtc tttgacagcc ctaccctgct atcctgacct caacacaagg
     1621 aggcacgggt atgaaagtca tctctatttt tctggcgcta atcaacttcc ttacaggggt
     1681 tttgttaatt ctctcctgca tctctgccaa tgaaaccttc gcctgggtcg cttggaaaac
     1741 tggcttgggc gttctgggcg tcacctttgg cattctcact ttcaaagata gcgcacagcc
     1801 tgtcagtcaa cgcaagatga ttttgtatgg cttgctcttg gtcgtggtcg gcgtttccat
     1861 cgtggcgtat ggcattcact ggtctgtcat cagcggtgac ccaaagcata ccgtcatggt
     1921 ggttggcagt agtttctttc tacacgggtt cacctcggtc ttaggcatgg caagcgatta
     1981 atctcaccgc ataggtcttc ccgctctgct ctctgtagca aaagaaaaat ctctttccta
     2041 attcagaaca aacgtgctat tattgcgaga tgttcacacg tctcgaaaaa ctttatcacc
     2101 aatttcctcc ccgctactgg gtcatcgtcc tcaccttctt tattgatagc atcggcggga
     2161 cgctgctctt ccccttcttc tctttgtata ttacccaaaa attcaacgtc ggcatgacgc
     2221 aagctggcat ggtacttgcc atgttttcca tcttcggcat cgtcggcaat atgattggcg
     2281 gcgccctcac cgaccgactt ggtcgccgta aactaattct cttcggcttg gtctttagcg
     2341 cactgagcac actgtcgctt gggttggtca actcgctggc agtcttattt ccgctcgccg
     2401 cgtttatcgg tctgctttca gatattgccg gtccagccaa ccaagccatg atggcagaca
     2461 tcctcgacga aaagcaacgc caagaaggct tcgggctgat gcgcgttatc cgcaacctag
     2521 catggatcat cggtccgagc attggcgggt ttcttgccaa tcgctccttc atgctgctct
     2581 ttatcatcga tgccgttgta agctgcatcg tcgccgttat cttcttcact tacatgccag
     2641 aaacaaaacc acagcatcac gaagaagaac ataaagaaca tgaatccatc ctaaagacca
     2701 tcgccgggta cggtatcgtc ctcaaagact tagccttcat ggcgttcatc atcgccgcca
     2761 ttctaatggg cgcggtctat caacagatgt acaactccct ctcagtctat ctgcgcgaca
     2821 atcacggcat caatccgcaa ggctatggtt ttctcatgac caccagcgcc atcacggtca
     2881 tcttgtttca attctgggtt tcgcgcaata tgaaacatcg tccgcctttc cttattatgg
     2941 cggtcggcac cctgttttat atgcttggct ttagcatgtt tgggttcgtc tctgtttatt
     3001 ggcttttt
//
LOCUS       DAOREF010000332         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21896_length_3008_cov_2.579072, whole genome shotgun sequence.
ACCESSION   DAOREF010000332 DAOREF010000000
VERSION     DAOREF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21896_length_3008_cov_2.579072"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKI33_07890"
     CDS             <1..470
                     /locus_tag="PKI33_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36967.1"
                     /translation="HKPKILFLDEPTLGLDVTMQKRIRSFIAEYNKRYGATVLLTSHY
                     MADVEALCKRVIVIHHGQLLFDGDLAALVQKFSAFKTVAFDIDNPNHDFSQYGDVVSI
                     DGLHVTLRVPKEKTSAVTTRLLSDLAVDDLTVEATPIEDVIESVFSVKKEALE"
     gene            467..1270
                     /locus_tag="PKI33_07895"
     CDS             467..1270
                     /locus_tag="PKI33_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNM36968.1"
                     /translation="MKRYFDFYFTSMKMAVIEQWQYPIANYFYMIGMIAEPVIYMVVW
                     SAVATQQGGTVGGYTPGAFAAYYIVWTLVRSMNVVFTPYGWEWRIRQGQLSMSLMRPI
                     HPLHNDVAFFAGWKVVVIILWIPLALALTLFFKPDLHPTLLNGIVFFFAIWFAYLIRT
                     MTLSLLGMVTFWTTRVSALFELYFAAELVLSGRLVPLSLMPEWVQKLAWFFPFRWAFG
                     YPIEALIGNMPVNDLFIGLGMQLVWILFGVMAVSLVWKVAIRRFSAVGG"
     gene            1272..2057
                     /locus_tag="PKI33_07900"
     CDS             1272..2057
                     /locus_tag="PKI33_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNM36969.1"
                     /translation="MTGIKLAFNYLRIGIMNEFQYRANLYIQILQTFIALGTGLIGLN
                     LVFAQTSQLGGWGKADLLAVMGVFTMTGGFIRAAIQPNMERLMDEIREGTLDYALTKP
                     ADSQLLVSVREFRLWQLVDVITGTIVLTVALIELKELINVWTVLGFFSALVMGAIMVY
                     SFWLIITSTAFWFVRVNEIANLFEGLYAAGRWPVDIYPNWLRYGLTFLVPVAFAVTVP
                     ASAITGRLTLQTWLLALAVTSILFILARTIWTIGVRNYSGASA"
     gene            2126..2842
                     /locus_tag="PKI33_07905"
     CDS             2126..2842
                     /locus_tag="PKI33_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36970.1"
                     /translation="MKHRIFQNKFIILFMVLMLASCSMADLSPATSTPKPTSTIYITL
                     TPIDTPTNTFTPTLYPSATIVKIATWDPNQPTFTPLPQLANLFPTATSSKPGPGFASV
                     SYSPKKIYWGGCNPNSVSITARVDDVEEVTSVVIFVRVKDLTEEDYTPWTNGDVMLNR
                     GQGEFTTVLYGSKINGHDHYLRSWVYFQLVATNIQGEEIGRTRIYEKAFDMYPCPCLT
                     PLTGCPITTPKPSATPKSKP"
BASE COUNT          692 a          764 c          668 g          884 t
ORIGIN      
        1 tacacaagcc aaaaatcctc ttccttgacg agccgacgct aggtctcgat gtgaccatgc
       61 aaaagcgcat ccgctcgttt attgcggaat acaacaaacg ctacggtgcg accgtgctgc
      121 tcaccagtca ctacatggca gatgtggaag ccctgtgcaa gcgcgtcatc gtcatccacc
      181 acggacagtt gcttttcgat ggcgacctgg ctgctttggt gcaaaaattc tccgccttta
      241 aaacggtcgc ctttgatatt gacaatccca accatgactt cagtcaatat ggtgatgtgg
      301 tctccattga tggcttacat gtgaccttgc gcgttcctaa agagaaaacc tccgccgtca
      361 cgacccgtct gcttagcgac ctcgctgtag atgatctcac tgtcgaagcc acgcctattg
      421 aagatgtgat cgaatccgtc ttttcggtca agaaagaggc gctggaatga agcgatattt
      481 tgacttctat ttcacatcca tgaagatggc tgtcatcgaa cagtggcagt atcccattgc
      541 caactatttc tatatgatcg gtatgattgc tgagccggtt atttatatgg tggtctggtc
      601 tgcggtggca acccagcaag gcggcacagt gggcggatat acccctggcg cgttcgctgc
      661 ctactacatt gtgtggactc tcgtccgtag catgaatgtg gttttcacgc cctacgggtg
      721 ggaatggcgt attcggcaag gtcaactgtc gatgagtctg atgcgcccta ttcatccgct
      781 tcacaacgat gtggcattct tcgctggttg gaaagtcgtc gtcattatct tgtggattcc
      841 gctggcattg gcgctcacct tgttcttcaa gcctgacttg catccgacct tgctaaatgg
      901 catcgtattc ttttttgcga tttggtttgc gtacctgatt cgcaccatga ccctctctct
      961 gcttggcatg gtcacttttt ggacgacacg tgtcagcgcc ttgtttgagt tgtattttgc
     1021 cgctgagttg gtcctttcag gtcgccttgt tccgctttct ttaatgccag agtgggtgca
     1081 aaaattagca tggttcttcc cattccgttg ggcatttggt tatcccattg aggcgctgat
     1141 cgggaatatg cctgtcaatg acctgttcat tggcttgggt atgcagcttg tatggatcct
     1201 atttggtgtg atggctgtaa gcctggtgtg gaaagttgcc atccgccgct tttcggcagt
     1261 aggaggttaa tatgaccgga ataaaacttg ccttcaacta cctgcgaatt ggcatcatga
     1321 atgagtttca ataccgcgcc aatctttata ttcagattct tcagaccttt atcgccttgg
     1381 gcacggggtt gattggtttg aatttggtct tcgcgcaaac gtctcaactc ggcggttggg
     1441 gcaaagcgga cctgttggct gttatgggcg ttttcactat gacaggcgga tttatccgcg
     1501 ctgccattca gcctaatatg gagcgcctca tggatgagat ccgcgaaggg acactcgact
     1561 atgccctcac caaacctgcc gattcgcagt tactcgtcag tgtgcgcgag tttcgccttt
     1621 ggcaattggt cgatgtgatt acaggcacca tcgtgttgac tgtggcactc atagaactca
     1681 aagagctgat taatgtatgg accgtgctag gctttttctc cgcgcttgtt atgggcgcga
     1741 tcatggttta cagtttttgg cttatcatca catccactgc tttttggttt gtgcgtgtaa
     1801 atgaaattgc taaccttttc gaaggtctat atgccgccgg acgctggccc gtggatatct
     1861 accctaactg gctacgctat ggcttgacct ttctcgtccc tgtcgccttt gctgtgactg
     1921 tgcccgcttc cgctattacg ggacgattga ccctgcaaac ctggctcctt gctttggctg
     1981 taaccagcat cctcttcatc ctcgccagaa cgatatggac catcggcgta cggaactact
     2041 ccggcgcttc tgcctaacaa aaaggacggt tactttcaaa gtaaccgtcc tttttacaca
     2101 ttagatttca tagtattata gcgggatgaa acatcgtatt tttcaaaaca aatttattat
     2161 cttgtttatg gtattaatgc tggctagttg ttctatggca gatctgtcac ctgccactag
     2221 cacaccaaaa cctacatcta ctatttacat tacacttact cctattgata cccctacaaa
     2281 tacatttacc cctactcttt atccttcagc aacgattgta aagattgcaa cctgggatcc
     2341 taatcagcct acttttaccc ccctaccgca acttgctaat cttttcccaa ccgctacatc
     2401 ttcaaaacca ggtccgggtt ttgcttcagt ttcatattcg cccaaaaaga tttattgggg
     2461 cgggtgtaac ccaaactcag taagtatcac agcgagagtg gatgatgtgg aagaagttac
     2521 tagtgtggtt atattcgttc gtgtaaaaga tttaacagaa gaggattaca ctccatggac
     2581 caatggagac gtaatgttaa atcgtgggca aggcgaattc acaacagtac tttatggcag
     2641 taaaattaac gggcatgacc attatttgcg ctcttgggta tacttccaat tagttgctac
     2701 taatattcaa ggtgaagaaa taggacgcac tcgaatttat gaaaaagcct tcgatatgta
     2761 tccatgtcca tgccttaccc ctcttacagg ctgcccaata acaactccaa aaccatctgc
     2821 cacacccaag agtaaaccat gagaaaaaac attgcgcttt ccctgctttt aattctttcc
     2881 cttgcttgtt cagtagagac atttatcccg gctgctgcca ctccgacccc tgcaccaaca
     2941 cagacagata cagctacgcc tcttcctacg cctacacctg tcaccccaac cctcaccttt
     3001 acattaac
//
LOCUS       DAOREF010000333         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_21972_length_3002_cov_4.199186, whole genome shotgun sequence.
ACCESSION   DAOREF010000333 DAOREF010000000
VERSION     DAOREF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21972_length_3002_cov_4.199186"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="miaA"
                     /locus_tag="PKI33_07910"
     CDS             <1..854
                     /gene="miaA"
                     /locus_tag="PKI33_07910"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNM36971.1"
                     /translation="LAEQMNGEIVSVDSRLFYRGMDIGTAKPSKEEMARVPHHLIDIV
                     TPDETLSLAVFQEMAQTIIAGIHARGRLPFLVGGTGQYIRAVTQGWSPPEVTPNEQMR
                     AVLETLQEEKGKEWLHAKLQILDPEAAQAIDLRNVRRTIRALEVIFSTGRRFSEQRGI
                     GDSPYHLITIGLARPREELYQRVDERIEKMFADGLLNEVKSLLDQGYSPTLPSMSAIG
                     YRECVSVVQGSMTEEQAKVEMRRLTRVFVRRQGNWFKESDPSIHWFHPARENLLNEIE
                     AMIRNSI"
     gene            956..2656
                     /locus_tag="PKI33_07915"
     CDS             956..2656
                     /locus_tag="PKI33_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNM36972.1"
                     /translation="MSNKPWLAHYDKGVPQTIEYPKAPLFHFLEEAARKYPDRACTIF
                     KGAVISYREMNERSNAVAAALVEMGVKKGDRVGIFMPNLPQFVSVYFGILKAGGVVIA
                     VNPTYPVEEILTPVNDAGIEIMFTLSRFYGKVKEVREKSGLKKIIVSNLKEALPPFTR
                     LLFTLLKEKKDGDKLDSLDSRDVWLTDLLKKYAGASKPNVDIQPDDTALFQYSGGTTG
                     VPKGAVAMHRNVVANTLQIKSWMTGLDEGKEVVLMGIPLFHVYGMVAGMNFAMATGSS
                     MVMIPNARDLKDVLENIAKYKATIFPGVPLLYNGINNHPDVKAGKYDLSSIKACISGS
                     AALMRETKEEFERLTGGKVFEGFGMSEAPTAVTCNPLNGVNKTGSIGMPLPDVDMKII
                     SLDDGETEMQLGEIGEIVVNGPQVMKGYHNMPTETANSLRQLKDGKTWLFTGDIARMD
                     EDGYFYIVDRKKELIKPGGFQVWPREVEEAIMDNPKVLEVGVGGIPDPKSGETVKAWV
                     VLKPGESMTEDELKEFCKERLAPYKVPRYVEFRTELPKTTVGKILRRELVRQHKEGGK
                     "
     gene            complement(2719..>3002)
                     /locus_tag="PKI33_07920"
     CDS             complement(2719..>3002)
                     /locus_tag="PKI33_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNM36973.1"
                     /translation="LVYGQQEELIFLGREISNQRDYSEAVAEQIDREVRKLVEDAYKQ
                     ARATVKKYRKQLDAVAKKLLEVESITREEFEQIFPPPLGKKSGTPQLTA"
BASE COUNT          784 a          711 c          799 g          708 t
ORIGIN      
        1 gcctcgccga gcaaatgaac ggcgagatcg tctcggttga ttcgcgcctg ttctaccgcg
       61 gcatggatat tggcactgcc aaaccatcca aagaagagat ggcacgtgtg cctcatcact
      121 taattgacat cgttactccc gatgagacct tgagcttggc ggtctttcaa gagatggcgc
      181 aaaccattat tgcaggcatc cacgcccgcg gacgattgcc attcctcgtt ggtggcacag
      241 ggcaatacat ccgtgcggtg actcaaggtt ggagtccgcc agaggtgacg ccgaatgaac
      301 agatgcgcgc cgttcttgaa accctgcaag aggaaaaagg gaaagaatgg ctgcacgcta
      361 aacttcaaat cctcgaccct gaagcagctc aagccattga cttgcgcaac gtccgccgca
      421 cgatacgggc tttggaggtc atcttcagca cagggcgaag attctctgaa caacgtggca
      481 ttggagactc gccgtatcat ttaattacga ttgggctggc acgtccgcgc gaggagttgt
      541 atcaacgggt agacgaacgc attgagaaaa tgttcgcaga tggcttactg aatgaagtaa
      601 aaagcctgct tgatcaggga tactccccca cattgccaag catgtctgcg atcgggtacc
      661 gagaatgtgt cagcgtggta caaggctcca tgacggagga acaagccaaa gtcgaaatga
      721 ggcgtttgac tcgcgtcttt gtgcgccggc agggaaactg gttcaaagag tcagacccat
      781 cgatccattg gtttcatcct gcccgtgaaa atttactcaa tgaaattgaa gcaatgattc
      841 gtaactctat ataaatacag gggttgtaat tgacttttaa tatcttcacg tatatacttc
      901 aaacctaggt agagagtcat cttcagttat ccaacaaacc aatggaggag gaagtatgag
      961 caacaaaccg tggttggcgc actatgataa aggtgtgcca caaacaatcg aatatcccaa
     1021 agcgccgttg tttcactttt tggaagaagc cgcgcgcaaa tacccagacc gtgcctgtac
     1081 gatatttaaa ggcgcggtca tttcgtatcg tgagatgaac gaaagatcca atgctgtggc
     1141 ggctgccttg gttgagatgg gtgtcaagaa aggggatcgg gttggtattt ttatgcccaa
     1201 cctgccgcag ttcgtaagtg tgtattttgg tattctcaaa gctggtggcg tggtgatcgc
     1261 tgtgaatccc acatacccgg tagaagagat tctcaccccg gtaaatgatg ccgggatcga
     1321 gatcatgttc acgctttcac gtttttatgg caaggttaaa gaagtacgcg aaaagtcggg
     1381 gcttaagaaa atcattgttt ccaatttaaa agaagctttg ccgcctttca cccgtctgtt
     1441 gttcactctt ctaaaagaaa agaaggatgg cgacaaactg gactcgctcg atagtcgcga
     1501 tgtatggctg actgatttgc tgaagaaata tgccggcgcc tcaaaaccga acgtcgatat
     1561 tcagccggac gatacggcac tcttccaata ttctggcgga acgaccggcg tgcccaaggg
     1621 cgctgttgcc atgcaccgta atgtagtggc aaatacgctg caaatcaaaa gctggatgac
     1681 cgggcttgat gaaggcaaag aagtggtctt gatgggcatt ccgctcttcc atgtatatgg
     1741 tatggtggcg ggcatgaact ttgccatggc gaccggttcc agtatggtca tgatcccgaa
     1801 tgcgcgtgac ttaaaagatg tattagagaa tatcgccaaa tacaaggcga ccatcttccc
     1861 cggtgtgcca cttttgtata acggcatcaa caatcacccg gatgtgaaag ctggcaagta
     1921 tgacttgtcc tccatcaagg cttgtatctc tggttctgcg gcattgatgc gtgagaccaa
     1981 agaagaattt gaacgcttga ccggtggtaa ggtattcgaa gggttcggca tgtctgaagc
     2041 ccccaccgct gtgacttgta acccgctcaa cggcgtgaac aagaccggct ctattggtat
     2101 gcctttgccc gatgtagata tgaagatcat cagtttggac gatggcgaaa ctgaaatgca
     2161 attgggcgag atcggcgaaa tcgttgtaaa tggtccgcag gtgatgaaag gctatcacaa
     2221 catgccgacc gagaccgcca actcgctgcg tcaactgaag gatggaaaga cctggctctt
     2281 caccggcgat atcgctcgca tggatgaaga tggctacttc tatatagttg atcgcaagaa
     2341 ggaactcatt aaaccgggcg gcttccaagt atggccacgt gaagtggaag aagccattat
     2401 ggataacccc aaggtcctcg aagtgggcgt gggaggcatc ccagacccga agagcggtga
     2461 aacggtcaaa gcctgggtgg tgctcaaacc cggtgaaagc atgaccgaag atgaattgaa
     2521 ggaattctgc aaagagcgcc tggcacccta caaagtgccg cgctatgtag aattccgcac
     2581 cgagttgccc aagacaaccg ttggcaagat cttacgtcgc gagttggtcc gccagcataa
     2641 agaaggcggc aagtagttcg taattaataa aataaaagag cctctcaaat tgagaggctc
     2701 tttttgtatg acagatattt atgcggtcaa ctgcggggtg ccgctcttct tgccaagcgg
     2761 aggcgggaag atctgctcaa actcttcgcg ggtgatggat tccacttcga gcagtttctt
     2821 ggctactgca tccaactgct tgcggtattt cttcaccgtc gcgcgagctt gcttgtacgc
     2881 atcttccacc aacttgcgga cttcgcggtc gatctgttca gccacagctt cggagtaatc
     2941 acgctggttg gagatttcgc gcccgaggaa gatcaattct tcctgctgcc catacacgag
     3001 cg
//
LOCUS       DAOREF010000334         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22042_length_2996_cov_3.586535, whole genome shotgun sequence.
ACCESSION   DAOREF010000334 DAOREF010000000
VERSION     DAOREF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22042_length_2996_cov_3.586535"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="PKI33_07925"
     CDS             complement(<1..1666)
                     /locus_tag="PKI33_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNM36974.1"
                     /translation="MSGLHSGTVTFLFTDIEGSTRLWEEHPEAMKTALAKHDSVLRES
                     IESSHGQIIKTTGDGVHAVFETAIDSIRASIAAQSGFQTPEFLNKSGVLLRVRMGIHT
                     GEAELRDGDYYGSVLNRAARLMSMAHGGQILISNATSELIRDGLPANVTLRDLGEHRL
                     RDLTRPEHIFQLVHPDLPGEFPPLKTLDAFPNNLPVQLTSFIGREKEIAEIKSALGSA
                     RLVTLTGSGGTGKTRLSQEVGMQLLTNFQHGVWLVELAPLSDESQIIPALAQVFGLQE
                     LPFNPLAKLVEDYLRDKKLLLILDNCEHLIAGCARLADHLLHQCAGLKILASSREALG
                     IAGEVAYRTPSLSSPALGGFPAAEDGGLSKKILEYESTSLFVDRARAANSKFALTDSN
                     ASSIAQICHRLDGIPLAIELAAARTKLLSAEQIATRLDDMFRLLVGGSRTALPRQQTL
                     RALIDWSYDLLSDEEKRLLQFASVFVGGWTLEALEFVAEEPSTLELLEQLVNKSLVVT
                     EEHKGEMRYFLLETIRQYGREKLFEAKQASAARDRHFIYYKNLTNGL"
     gene            1774..>2996
                     /locus_tag="PKI33_07930"
     CDS             1774..>2996
                     /locus_tag="PKI33_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36975.1"
                     /translation="MKRILEIFKINPGEERMSTLLVGLMLLTSAGSALGGNAVSALFF
                     NRFGVESLPVMYMLLGTLTFMTSMGITIIMGRFSKKHLFTYLPVILGMVLLAGWITLG
                     LGIKWIFAVMWLGMNVIGSLQGVWVWGLAGAACDTRQAKRLFPLFSAGGILGAVLGGF
                     LTPPLAGRLHSETLVLLWAGVFLLCFYLGRTLVGGAVPETSSRAGNANILTEMQRGYQ
                     FVRRSSIMQWVAYSAFLFSLCYFFLDLQFSRGASARFPSADQLAGFLGLFQSINTIIA
                     LIVSLFLANRLFARFGIMPMLLLFPVIYLLGFGVLSFFAPFPALVSVRFLQMVWMQGI
                     AGTAWQTLFNVVPANQRDQVRTFIGGAPEQLGVFAAGLLLFLGDRALNSQQLNIVALF
                     SAAALVYVIWRASKA"
BASE COUNT          618 a          816 c          793 g          769 t
ORIGIN      
        1 ataagccatt cgttaaattt ttatagtaga tgaaatgccg atcacgcgcc gccgaagcct
       61 gcttcgcttc gaacagcttt tccctcccgt attgccgaat cgtctccaac aggaagtaac
      121 gcatctcgcc tttgtgttct tccgtcacca ccaacgactt gttgacgagt tgctccaata
      181 actccagcgt agacggttct tccgcgacga attccaacgc ctccaacgtc cacccgccaa
      241 cgaagaccga ggcgaattgc aagagccgtt tttcttcgtc tgatagtaag tcgtaactcc
      301 aatcgatcaa cgcgcgcaga gtctgttgac gcggtagggc ggtgcgactt ccgccgacaa
      361 gcagtcggaa catgtcatcg agccgtgtgg cgatctgttc cgcggaaagg agtttcgtcc
      421 gtgcggcggc aagctctatc gccaaaggaa tgccgtcgag gcggtgacag atctgggcga
      481 tggaagaagc gtttgaatcg gtgagagcaa acttcgagtt ggcggcgcgg gcgcggtcca
      541 cgaaaaggct ggtcgattcg tattccaata tttttttgct caagccgccg tcctcggcgg
      601 cggggaagcc accgagagca ggggaagata acgaaggtgt gcgatacgcc acttcgcccg
      661 cgatgcccaa cgcctcgcga ctgctcgcta aaatcttgag tccagcacat tgatggagca
      721 gatggtccgc aagccgcgcg caacccgcga taaggtgctc gcagttatcg agaatgagca
      781 gaagtttttt gtcgcgcagg taatcttcca ccagtttcgc cagcggattg aacggcaatt
      841 cctgcaatcc aaacacctgc gccagtgcag ggatgatctg cgactcatcg ctcaacggcg
      901 cgagttccac cagccacacg ccatgctgga agtttgtcaa cagttgcata ccgacttcct
      961 gtgagagtct ggtcttgccc gtgccgcctg agccagtgag cgtaacgagc cttgcagaac
     1021 caagcgcaga ttttatttcg gcaatctctt tttcgcgccc gatgaatgaa gtcagttgta
     1081 cgggcaggtt atttggaaaa gcatccaatg ttttaagagg cgggaattca ccgggaagat
     1141 cagggtgaac cagttgaaaa atgtgttccg gtcgggtcaa gtccctgagg cgatgttcgc
     1201 ccagatcccg cagggttaca ttcgcaggca atccatcgcg gataagttcc gacgtggcgt
     1261 tcgagatcaa aatttgtccg ccatgcgcca tggacatcaa ccgggctgca cggttcagta
     1321 cgctgccata ataatcgccg tcgcgcaatt cggcttcgcc ggtgtgaatg cccatgcgga
     1381 cgcgcaacaa gaccccggat ttatttaaga actccggggt ctgaaacccg ctttgtgcgg
     1441 caatggatgc gcggatggag tcgatggcag tttcgaagac cgcatggacg ccatctcctg
     1501 ttgttttgat gatttgcccg tgacttgatt caatggattc ccttagaacc gaatcatgtt
     1561 tcgccagcgc ggttttcatc gcttcgggat gctcttccca aagccttgta gacccctcta
     1621 tatcggtgaa taggaatgtg accgttcctg agtgcaatcc gctcatgacc aatctccaaa
     1681 acgaaccggt ctgacttatt tggagtattc taatccacat ttgaaagtaa aatacctctg
     1741 atacgcccgt gcctcgaacg gaacaggttt tgtatgaaac gaattttaga gatcttcaaa
     1801 atcaacccag gcgaagaacg catgtccaca ttgctggtcg ggttgatgct gctcacctcc
     1861 gccggcagtg cgcttggagg gaatgccgtc tcggctttgt tcttcaaccg tttcggtgtg
     1921 gagtctctgc cggtcatgta tatgcttttg gggacgttga ccttcatgac ttcgatggga
     1981 atcaccatca tcatgggacg gttttcaaaa aaacatctct tcacctacct gcctgtcatc
     2041 cttggcatgg tattgcttgc gggatggata acattgggtc tggggattaa atggatcttc
     2101 gccgtcatgt ggctgggtat gaacgtcatt ggaagtttac agggagtatg ggtctggggg
     2161 ctggctggcg cggcttgtga tacgcgccag gcaaaacgcc tgttcccctt gtttagcgcg
     2221 ggcggtattt taggcgctgt gctcggcggt tttttaaccc cacccctggc gggcaggctt
     2281 cattctgaaa ctctcgtact gctatgggct ggcgtattcc tcctctgctt ttaccttggc
     2341 cgcacgctgg ttggcggtgc tgtgcccgaa acatcctccc gcgccggtaa tgcaaacatc
     2401 ctcacggaaa tgcagcgcgg atatcaattc gtgcgccgtt catccatcat gcaatgggta
     2461 gcgtacagcg cctttttatt ttcactgtgc tacttcttcc tggacctgca attctcacgc
     2521 ggcgccagcg cccggttccc cagcgcagac caacttgcag ggttcctcgg tctattccaa
     2581 agcatcaaca cgatcatcgc cttgatcgtt tcgttattcc ttgccaatcg tctgtttgcc
     2641 cgctttggca tcatgcccat gttgctgctc ttccccgtca tttatttgct ggggtttggc
     2701 gtattgtcct tttttgcgcc cttccccgcc ttggtgtctg tgcgcttttt gcaaatggtc
     2761 tggatgcaag gcattgcagg aaccgcatgg caaactcttt tcaatgtggt gcccgccaat
     2821 caacgcgacc aggtgcgcac cttcattggc ggcgcgcccg aacagttggg tgttttcgct
     2881 gcgggcttgc ttttattcct tggcgaccgc gccttgaatt cgcagcaact aaatatcgtc
     2941 gccctttttt ctgccgcagc cttggtttat gtaatctggc gcgccagtaa agccta
//
LOCUS       DAOREF010000335         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22097_length_2992_cov_4.294178, whole genome shotgun sequence.
ACCESSION   DAOREF010000335 DAOREF010000000
VERSION     DAOREF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22097_length_2992_cov_4.294178"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..241)
                     /locus_tag="PKI33_07935"
     CDS             complement(119..241)
                     /locus_tag="PKI33_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36976.1"
                     /translation="MALILPALDRSLIPQIITRTKAVTEESRLTEAARDVQGKW"
     gene            240..884
                     /locus_tag="PKI33_07940"
     CDS             240..884
                     /locus_tag="PKI33_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HNM36977.1"
                     /translation="MTASPLFEFALLSFISMFTMVNPIGVVPVFTAMTAKLTPQESHR
                     VARKATFTGAVILFAFALTGQWIFRFFSISVDSLRVVGGVIFFFMGYEMLQARLTRTQ
                     FDDETNHEYINDISITPLGIPIICGPGAITNAILLMNEADTMVEQGIVLGVIAIILLF
                     TYLLLLGAKQVTRIIGENGNKVMLRLMGLIVMVIAVEFFFAGLTPIVRKMLSGG"
     gene            complement(968..2455)
                     /locus_tag="PKI33_07945"
     CDS             complement(968..2455)
                     /locus_tag="PKI33_07945"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNM36978.1"
                     /translation="MKNKYILLSAIILMALSCKALTPSTPTPIPSTEAVPTEDLPATE
                     PAVDEPIDPLAGSPGLGDSLYPDFGNGGYDATSYLLDITVHDVKTSDLTAITTIEAVA
                     TQDLSQFNLDFAGFEITSITVNDEAAEFERDGQELTVIPAQALANGEAFTTVLEYQGV
                     PASMTSKALPFPTGWIAYENGIFVLSEPDGSASFYPVNDHPLDKAKYTIMVTVPKPYE
                     VGANGVLEETIDNGETTTYTFTVRDEMASYLTTININEFNTETMTSDGGISIRNYYAT
                     SLPEGVNKPFARQGEMIDYFSELFGPYPFEVYGALVMDTEFGAALENQTMSIFGIDMV
                     DLEDIEGTEAVVAHELVHQWFGDSVSVGDWGSIWLNEGFASYGEGLWEEHLNGRDALD
                     EWVNGVYEEVKAYPEFYPPPGNPPANDLFNGGVYLRGGLTLHALRLEVGDDVFFEILR
                     TYYDRYKYGNAVTDDFIAVAEEVSGKDLGDFFNAWLYEEQLPELP"
     gene            complement(2445..>2992)
                     /locus_tag="PKI33_07950"
     CDS             complement(2445..>2992)
                     /locus_tag="PKI33_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNM36979.1"
                     /translation="ADPFIVQLAALLHDLDDWKFNEAGDETPHRAKAWLASCSVSPYI
                     ADTVCKIIMHISYKGAGVENKMDSLEGFIVQDADRLDAIGAIGIGRAFAYGGYKNRMM
                     YDPDSPPAMHASFEEYKNSKSATINHFYEKLLLLKDRMNTSTAKRIAEERHDVMVKFL
                     DQFMLEWEGADIMNSSGTYEK"
BASE COUNT          726 a          720 c          741 g          805 t
ORIGIN      
        1 ttgtgccgac taacatcggc ttccctcata acgctgggag tgcggttcag actactagaa
       61 aatcattcac ctgaacggct cgagagggaa cttcaactgg gttcggtcga gtgcaatttc
      121 accacttgcc ttgcacatct ctggcggctt ccgtcagcct actttcctct gtcactgcct
      181 ttgtgcgggt aattatctgc ggaatcaggc tcctgtcaag tgcgggtaaa atcaacgcca
      241 tgaccgcatc tcctcttttt gaatttgccc tgctcagctt tatttcgatg ttcaccatgg
      301 tcaacccgat tggggttgta cctgtcttca ctgccatgac cgccaagctg actccgcaag
      361 agtcgcatcg ggtggcacgc aaagcgacct ttaccggtgc cgtgattctc ttcgccttcg
      421 cgttgaccgg tcagtggatc tttcgcttct tctcgatctc tgtggacagt ttacgggtag
      481 tgggtggtgt gatctttttc tttatgggtt atgaaatgtt gcaagcccgc ctcacgcgga
      541 ctcaatttga tgatgaaacc aatcatgaat acatcaacga tatttccatt acgccgttgg
      601 gcattcccat catctgcgga ccgggcgcca ttaccaatgc tatccttttg atgaacgaag
      661 ccgatacgat ggtagaacaa ggcattgtgt tgggagtgat cgctattatt ttgcttttta
      721 catatttatt attacttggc gcaaaacaag tcacacgcat tattggggaa aatgggaata
      781 aggtcatgtt gcgtttgatg gggttaatcg taatggtgat cgccgtggaa tttttcttcg
      841 ccgggttgac accaatcgta cgaaaaatgc tttcgggtgg ctaaaggctc ggagcgctaa
      901 agcaggtttg agttaagttg gaagcgtaaa agagacagtc ccattacatg ggactgtctc
      961 ttttatccta tggaagttca ggaagttgtt cttcgtacaa ccaggcattg aagaaatcgc
     1021 cgaggtcttt gccgctgact tcttcagcaa cagcgatgaa atcatcggtg acggcgttgc
     1081 catatttata acggtcataa tatgtgcgca gaatctcaaa gaacacatca tcgccgacct
     1141 caagccgtaa ggcgtggagc gtgagcccgc cgcgcaagta aacgccgcca ttgaaaagat
     1201 cgttggctgg ggggttgcca ggcggagggt aaaactctgg gtatgccttc acttcttcat
     1261 acacaccatt aacccattca tcaagtgcgt cgcgcccgtt gaggtgttct tcccaaagac
     1321 cttcgccata tgaagcaaaa ccttcgttga gccaaatact gccccaatcg ccaacactga
     1381 cgctatcacc aaaccattgg tggacaagtt catgagcgac cactgcttct gtgccttcaa
     1441 tatcttccag atccaccatg tcgattccaa aaatggacat ggtttggttt tcaagcgcgg
     1501 caccaaactc tgtgtccatc accaaagcac catacacttc aaatggatac ggaccgaaaa
     1561 gctcgctaaa gtagtcgatc atctcgccct gacgcgcaaa aggtttattg acaccctcgg
     1621 gcaaactggt ggcgtaataa ttgcgtatcg aaataccacc atcagaggtc attgtttctg
     1681 tattgaattc attgatgttg atggtagtga gatagcttgc catttcatca cggacagtga
     1741 aggtataggt ggtcgtctcg ccattatcga tggtttcttc aagaacgccg ttcgccccaa
     1801 cttcataggg tttgggcaca gtgaccataa tggtgtactt cgccttatcg agcggatggt
     1861 cattgaccgg ataaaaactg gcggaaccat cgggttcgct caagacaaag atgccgttct
     1921 catatgcgat ccaaccggtg gggaatggca acgctttaga cgtcatcgat gcgggaacac
     1981 cttgatactc cagcacggta gtgaaagctt cgccgttggc tagcgcttgc gctgggatga
     2041 ccgttaattc ctgcccgtca cgttcaaact ctgccgcctc atcgttgacc gtgatgctgg
     2101 taatttcgaa cccggcaaaa tccaggttga actggctcaa atcttgagtt gcaacagctt
     2161 cgatggtagt aattgccgtt agatcgctgg tcttcacatc gtggacggta atatccaaaa
     2221 gataggatgt agcgtcataa ccgccgtttc caaaatcggg gtaaagagaa tctcctaaac
     2281 ccggagaacc tgccaagggg tctatgggtt catcaacagc gggttctgtt gcgggtaaat
     2341 cttctgtagg cacagcctct gtggaaggaa tcggcgttgg cgtagatgga gtcaacgctt
     2401 tgcaagatag tgccatcaaa atgatcgcag aaagcagaat atatttattt ttcataggtt
     2461 cctgatgaat tcataatatc tgcaccttcc cattcgagca tgaactggtc gagaaatttc
     2521 accatcacat cgtgccgttc ttcggcaatg cgcttggcag tggacgtgtt cattcggtct
     2581 ttgagcagga gcagtttttc ataaaagtga ttgatcgtag cgcttttgct gttcttgtat
     2641 tcttcaaagc tagcatgcat ggcgggtgga gaatctgggt cgtacatcat gcgattctta
     2701 tagccgccat aagcgaaggc acgtccgatg ccgatggctc caattgcatc gagacggtct
     2761 gcatcttgga cgataaagcc ttcgagcgag tccattttgt tttctacacc ggcaccttta
     2821 taggaaatgt gcataatgat cttgcacacg gtgtcagcga tgtatgggct cacagagcaa
     2881 gaggcaagcc aggctttggc gcgatgcggc gtttcgtcgc ccgcttcgtt gaacttccaa
     2941 tcatcgaggt cgtgtaagag agcagcaagc tgaacaatga atggatcagc tt
//
LOCUS       DAOREF010000336         2989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22146_length_2989_cov_3.152011, whole genome shotgun sequence.
ACCESSION   DAOREF010000336 DAOREF010000000
VERSION     DAOREF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22146_length_2989_cov_3.152011"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="PKI33_07955"
     CDS             complement(<1..1472)
                     /locus_tag="PKI33_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM36980.1"
                     /translation="MNRLLILDDNERFAEVLQRSISGFEGYESETVQYVTTSEVAIEL
                     VKEATELQSPFNVLLIDQNLEAEMDGIQVMKEMLSIHSDADTIIFTGYDTPEDGLRAY
                     EAGASRYLPKPFEPEELEFVLKELMRSRKVRLAEARQRQQFRVATKIAEAVGASLNLE
                     DTMDAVLGTLYSVFDKTRLCVLLYDERQNALRFAPSTMKYYEIKNTQYSYQDTFPLNA
                     GSLACQAAQLTIRHKSMELVNVGDVTQDTEYLNLNPDTKSECCVGLLNMHGELLGVLA
                     LEREWLNGFDERDLDLIHITARHISIAIERAHQSEELKSKSAIAAQTSWAAALAHELN
                     NEVGKIANWAQVIQQVIGDDLPEIRNYAKNIEESAYYLSRSNPWSARSIKSFEIDQHI
                     KDVLEKLAPKKSLSIEFRLNAQGAKVKVSPAQFQFILKQLAGNAARAMGNNEDKKVLV
                     ETQLANLETVEILFQDFGPGISEENRMAIFNWQFTTKETGG"
     gene            complement(1481..>2989)
                     /locus_tag="PKI33_07960"
     CDS             complement(1481..>2989)
                     /locus_tag="PKI33_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM36981.1"
                     /translation="QQVDCVPSAEEAIEFLARIQPEFVLIDVGLPGMSGTELAHHIFE
                     NYPNVQVANATDWARASEVRTELEAVMDRGGNLFYKPLVPEDLARFLTGNVQRVEIAS
                     QDSAVPSKFASKWDAKHDIHKLLVTFRKHLGVEQVFLFSFDPAHRKVQIAERVGDGLI
                     NKNALSQLLHSPVRDVAEDGDAIFVDELTEREKKRFQYLIEFSPEAVSCIGVSVPTQS
                     SLKYALFAMDKQAKSFGEELRMYVEGMSLAVGATLDQIDLRERSALMQRSALIGNLAS
                     GMIHEINNLLAPLQYESNNLRKALAKIENDPTQSKYESIRNEVANIEQDIRQIVGTVG
                     TFGKIAKKSQVEILRVDEIIKDTLILLNQISKRSRVKMDFHAPEKMVVVRNQAVVLGQ
                     IFLNVCLNAIQQISEQGADHIRAIRIDMETRQETSQSEVCRILIRDTGPGIHASLWEK
                     IFEMGFSTRVDGSGIGLFVSRNLMEEIEGKIYVLDSHILHGTVFALEFPVHV"
BASE COUNT          738 a          705 c          641 g          905 t
ORIGIN      
        1 ccgccagtct cctttgttgt aaactgccag ttgaagattg ccatacggtt ctcttcgcta
       61 atgccgggac caaaatcttg aaacaggatt tccactgttt ctaggtttgc gagttgagtt
      121 tcaaccagaa cctttttatc ttcattgttg cccatagctc gagctgcgtt ccctgctagt
      181 tgtttgagaa tgaactgaaa ctgagcaggg gagaccttta cttttgcacc ttgagcattc
      241 aaccgaaatt cgatagaaag acttttcttt ggcgccagct tttcaaggac atctttaata
      301 tgttggtcaa tttcgaagct cttaatcgag cgagcagacc aaggattcga ccgtgagaga
      361 tagtaagcgc tttcttcgat gtttttggcg taatttctaa tttcaggaag gtcgtcgccg
      421 atcacttgtt gaataacctg tgcccagttt gcgatcttgc ctacttcatt gtttagctcg
      481 tgggcaaggg cagctgccca cgaggtttgt gctgcgatcg cggactttga tttcagttcc
      541 tcactttgat gagcacgttc aatggcgatg ctgatgtgac gtgcggtgat atggatgagg
      601 tcgaggtctc tttcatcgaa cccattgagc cattcacgtt ctaatgccag aacgcctaaa
      661 agctctccgt gcatattgag taaaccgaca caacattctg attttgtgtc aggatttagg
      721 ttgagatatt ctgtatcttg agtcacatcc ccaacattga ccagttccat gctcttgtgc
      781 cgaatggtca attgagcggc ttggcaggca aggctgccgg cgtttagcgg aaatgtatct
      841 tgataggagt attgagtatt tttgatctca taatacttca tggtgctagg cgcgaaccgt
      901 aaggcatttt ggcgctcatc atagagtagg acacacaggc gtgtcttatc gaatactgaa
      961 tacaatgtgc cgagtacagc atccatggtg tcttcgaggt tcaagctggc gcctacagct
     1021 tctgcaattt tggtggcgac tctaaattgt tggcgttgac gcgcctctgc tagacgcact
     1081 tttcttgagc gcattagttc tttgaggacg aattccagct cctctggttc aaaaggtttc
     1141 ggaaggtacc gtgaggctcc tgcttcatag gctcgtaagc cgtcttctgg ggtgtcgtac
     1201 cctgtgaaaa taattgtgtc tgcatctgaa tggatggata acatttcttt cataacctga
     1261 atgccatcca tctctgcttc caggttttgg tcgatcaaaa ggacattaaa agggctttga
     1321 agttcggttg cttcttttac aagctcaatt gccacttctg acgtagtgac atattgaaca
     1381 gtctcagact cgtagccctc aaacccgctg atactgcgct gtaaaacctc tgcaaagcgt
     1441 tcattgtcat caagaataag taaacgattc atagtaagcc tcatacatgg acagggaatt
     1501 caagggcaaa aaccgtgcca tgtaggatat gactatccaa tacgtagatt tttccttcaa
     1561 tttcttccat taagtttcgt gatacaaaca acccaattcc actgccatct acacgagtgg
     1621 aaaagcccat ctcaaagatt ttctcccaaa gtgatgcatg gattcccggt cctgtatccc
     1681 gaattagaat acggcagact tcgctctggc tggtttcctg tctggtttcc atatcaatcc
     1741 gaatagcacg gatatggtct gcaccttgct ctgaaatctg ttgaattgcg ttaagacaga
     1801 catttagaaa aatttgccct aatactacag cttggtttcg taccaccacc attttttcag
     1861 gcgcatgaaa gtccattttt acccgagagc gtttgctgat ttgatttagc aaaataagtg
     1921 tgtctttgat aatctcatcc actcgtaata tttcaacttg tgattttttt gcgatcttgc
     1981 caaaggttcc caccgtccct acaatctggc ggatatcttg ttcaatattt gccacctcgt
     2041 tgcgaatact ttcatacttc gattgagttg ggtcgttttc tatttttgca agtgctttac
     2101 gcaaattgtt agactcatac tgaagcggcg caagtaaatt gttgatttca tggatcatgc
     2161 cgctcgccaa attgccaatt aacgctgagc gttgcatgag tgctgatcgt tcacgcaagt
     2221 cgatctgatc cagcgttgcg cccaccgcca gagacattcc ttctacgtac atgcgcagct
     2281 cttcaccgaa agacttagct tgcttatcca tcgcgaataa tgcatatttc aacgaggatt
     2341 gcgttgggac agatacccca atgcatgata cagcttcagg gctgaattca atcagatatt
     2401 gaaaacgctt cttttctctt tcagtaagct catccacaaa tattgcgtcg ccgtcttcgg
     2461 caacatcacg tacagggcta tgtaaaagct ggctgagggc attcttatta atgagaccat
     2521 cacctacccg ttccgcaatc tggactttac gatgtgccgg gtcaaatgaa aaaaggaaaa
     2581 cctgttctac tcctaagtgc tttctaaacg tgaccagcaa cttgtgaata tcatgctttg
     2641 cgtcccattt tgatgcaaat tttgacggca cggcactatc ctgacttgct atttctactc
     2701 gttgaacatt accagttaga aatcttgcca agtcttcagg cacaagtggt ttataaaata
     2761 aatttccacc gcgatccatc acagcttcca actctgtgcg gacctcgctg gcgcgtgccc
     2821 aatcggttgc attcgctacc tgaacatttg ggtagttctc gaaaatgtga tgcgctaact
     2881 ccgttccact cattccgggt aaacccacat caattaatac aaactcaggc tgtattcgtg
     2941 ccaagaactc gatggcttct tctgcgcttg gcacacaatc tacttgctg
//
LOCUS       DAOREF010000337         2987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22167_length_2987_cov_4.485675, whole genome shotgun sequence.
ACCESSION   DAOREF010000337 DAOREF010000000
VERSION     DAOREF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22167_length_2987_cov_4.485675"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKI33_07965"
     CDS             complement(<1..306)
                     /locus_tag="PKI33_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNM36982.1"
                     /translation="MSQSRLDKLTASLRTSDLDAVILNPGPTLKYLTGISFHLMERPV
                     VLFVAPGKDPVLVLPELELPKLDLFPYKVQGVAYGENPAEWDDAFRKAAQALGVDGKR
                     "
     gene            348..2330
                     /locus_tag="PKI33_07970"
     CDS             348..2330
                     /locus_tag="PKI33_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM36983.1"
                     /translation="MSKKITTVTAFTLWSIVVLSSFYISQRPLFFQVMRSSFLFFGNI
                     TLTFLFLFNAAGLGYFIFKQFRLQTDLHERLLLGTGLGLGILGITGYGLGVLGLAQPT
                     ILFSLLIVSALFVLLKKFHTQIGSDLKNLFFLFSETKAALPKWTVWVVIITCCIGFIY
                     TFLPPAEGFDGLLYHLRLPELLLADNGLKPYNNFPLWFPSLIEGNYIWALSLNSERTA
                     QMLHWSYAMLSLIIVWKWSYQVFGAKAAWWSLVIIVSMPSLPLLSSWAYTDFGLIFFS
                     LSCTYCLWCWLESQKTSFIALSGVFAGFAMGVKYTSFILPLFCILYILLNSDNIYNKN
                     KSLFIFSSISILAALPWYIRNWIVMNNPFYPFVFGGRYWDPFLTQWYNNPGSGIGFDL
                     RELLFLPFTLTMGFRDVVYFDGRIGPLFLLLFPLVIWIIFKKYTSDIKGFKLLILVFF
                     FFTLSFAAWVFGIIQTVGLWQSRLLWPGFFHLVIPIGAAIANIQDLDIYRIRLSFITT
                     TLIGIVTFTSLLDSGLFLIARRPLINALGLETRAQYFDRIQPEYSEALKLVNNLPKES
                     MVYFLFEPRSYNMKPFVQPDPINSNLMHDYYIYKTPERIISSWKSSGYTHILVNNASA
                     LFIEKEEHIEESKIKEIINLLHLKKKGQSYSLYIIP"
     gene            complement(2320..>2987)
                     /locus_tag="PKI33_07975"
     CDS             complement(2320..>2987)
                     /locus_tag="PKI33_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM36984.1"
                     /translation="IQDAGGEAIAVPVDIVNPDEVDNMVQTAIDLYDRIDILFNNAGM
                     GRVGWYEEHEPDRDIDLLIKVNLIGTMRVTRMVLPHMVARREGHIINMISVAGLISPP
                     LITSYSASKFGLKAFNHSLRREVAPFGIKVSAIYPGPASTEFGSHVGKNAAYRSVRAS
                     LGGRLTSAYVAKRVLDVAKRPRKGLVIPWWFHFVIALETYFPFLVDWGSIIFSNLKHN
                     IKE"
BASE COUNT          844 a          587 c          599 g          957 t
ORIGIN      
        1 ccgcttccca tccacgccca aggcttgagc agcctttctg aacgcatcat cccactctgc
       61 aggattctca ccataggcaa caccctgtac tttgtatggg aacaagtcca gtttggggag
      121 ttcaagttcg ggcaagacaa gcactgggtc tttgccgggt gccacaaaca agaccacagg
      181 gcgttccatc aaatggaaac tgatgccggt aagatatttc agtgtaggac ccggattcag
      241 aataacggca tccaggtctg aggtccgcaa agaagcggtg agtttatcga gtcgagattg
      301 agacatagct ttattccttt ataattttat atggagaatt ttgaagaatg tctaaaaaaa
      361 ttactacagt tacagccttc accttgtgga gtattgttgt actctcatct ttttacatta
      421 gtcagagacc acttttcttc caagtaatga gaagttcatt tttgtttttt ggaaatataa
      481 cattaacatt cctttttctt ttcaatgctg cagggttagg ttattttata ttcaagcagt
      541 ttcgtttgca aaccgactta catgaaaggt tattattagg cacaggacta ggactaggca
      601 ttttagggat tacaggatat gggttaggag tattaggttt agctcaaccg actatcttgt
      661 tttcattgct tatagtttca gcattgtttg ttttattaaa aaaatttcat acacaaattg
      721 ggagcgatct aaaaaattta ttttttttat tctctgaaac aaaagcagcc ctcccaaaat
      781 ggaccgtatg ggttgtaata atcacatgtt gtataggatt tatctatact ttccttccac
      841 ctgcagaggg tttcgacggg cttctttatc acttgcgttt accagaactt cttctcgctg
      901 ataacgggct aaaaccttat aacaattttc ctctttggtt cccaagttta attgagggaa
      961 attacatttg ggcgctgagc ttgaatagtg aacgaacagc tcaaatgcta cattggtcat
     1021 atgcgatgtt gtcacttatc atagtatgga agtggagtta ccaagttttt ggagcaaaag
     1081 ccgcatggtg gtcattagta attatagtat ctatgccatc tctgccattg ctttcttcat
     1141 gggcatatac agatttcgga cttatttttt ttagcctatc atgtacatat tgcctttggt
     1201 gttggctaga atcgcaaaaa actagtttca tagctttgtc tggggtattt gcaggttttg
     1261 caatgggggt caaatatacc agctttattc tgccgttatt ttgtattctt tatattttac
     1321 ttaattcaga caatatttat aataaaaaca aaagtttatt tatcttttca agcatcagca
     1381 tcttagctgc tttaccatgg tacataagaa actggatagt aatgaacaat cctttttatc
     1441 catttgtatt tggcggcaga tattgggatc ccttccttac acaatggtat aacaatcctg
     1501 gtagtggcat tggctttgat ttgcgtgaac tattattcct gccctttacc ttaacaatgg
     1561 gatttcgtga tgtagtctat tttgacggtc gtattggacc gctttttctt ttattattcc
     1621 ctttggtgat ttggataatc tttaagaaat acacctcaga catcaagggg tttaagttac
     1681 ttatcttagt attttttttc ttcaccctat cttttgctgc ttgggtcttt ggcataatcc
     1741 aaacggtagg actttggcaa tctcgtcttc tttggcctgg ttttttccat ttagtaattc
     1801 caataggagc tgcaattgcg aatatacaag atttagacat atatcgtatt cggttaagtt
     1861 tcattaccac taccctaatt ggcatagtta cattcacttc tctgttggat tctgggttat
     1921 ttttaatagc tcgtagacca ttaataaatg cactcggctt agaaacacgc gcacagtatt
     1981 ttgatcgtat acaaccagaa tatagtgaag ctctcaagtt ggtaaataat ttaccaaaag
     2041 agtctatggt gtattttctt tttgaacctc gcagctacaa tatgaaacct tttgtccaac
     2101 ctgatccaat taattcaaac cttatgcatg actactatat atataaaaca ccagaaagaa
     2161 taatttcatc ctggaaatct agtggatata cacatatcct agttaacaat gctagcgcgt
     2221 tatttattga aaaagaggaa catattgaag aaagtaagat taaagaaatt ataaatttgc
     2281 ttcatttgaa gaaaaaaggg caaagctata gtctatatat cattccttga tattgtgctt
     2341 taaattagaa aatataattg aaccccaatc tacgagaaag gggaaatagg tttcaagtgc
     2401 aatgacaaaa tggaaccacc acggaatgac cagccctttg cgtggacgtt tagcaacatc
     2461 caaaacgcgt ttggcaacat aggcggaggt gagtcgaccg ccaaggctgg cgcgaacaga
     2521 tcgataggct gcgttcttgc ccacatgcga accaaattcg gtggaagcgg gtccggggta
     2581 aatagcgctg actttaatgc cgaatggcgc cacttcgcga cgcaacgaat gattgaatgc
     2641 cttgagccca aacttgctgg cagagtaact ggtaatgagc ggcggtgaga taagcccagc
     2701 cactgagatc atattaatga tatgaccctc acgacgcgcc accatatgcg gaagcaccat
     2761 gcgagtgaca cgcatcgtgc cgataaggtt cactttgatc aacaggtcaa tatcgcgatc
     2821 gggttcatgt tcttcatacc accctacacg acccataccc gcattgttga aaaggatgtc
     2881 gatgcggtcg tagaggtcaa tggcggtttg caccatatta tccacttcat cggggttgac
     2941 aatatcgaca ggaacggcaa tggcttctcc gcctgcatct tgaattt
//
LOCUS       DAOREF010000338         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22302_length_2978_cov_3.109477, whole genome shotgun sequence.
ACCESSION   DAOREF010000338 DAOREF010000000
VERSION     DAOREF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22302_length_2978_cov_3.109477"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1393
                     /locus_tag="PKI33_07980"
     CDS             14..1393
                     /locus_tag="PKI33_07980"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM36985.1"
                     /translation="MPAIDLTRLRKQANRLADFFFLPDEFMKHLREILEFYVNYTLRT
                     VENVAPGSNLKTYRTPPAVLTQIQNELRLKAEENPHFALELADILWDEGALETRLLAA
                     YLLGRIPPQEERLLPRITAWTQQIRDPEVRVALLTTSLTRMRKETPNQFLALVREYLH
                     PERSRSWSNGIQALIPMITDSDFENLPAIFDLVEPIIEEAPSTLQYDLTDLIVTLYRA
                     SASETIFMLKHILSTTENQMTAVTLRRISPDFPPPLQKELRDLLRPQPLTLIKPAEPD
                     DFIDESAPPEEKAKPARKPRKKKMDNSRIIYLHGSDSNSQSGKARQFAEKFPGMVTPD
                     FTGDFDERMAQLKKIIGRKKGWTIIGSSLGGLMGTVFTCQKPTHVRKLILLAPALLKD
                     PFGSYLNLESVSVPTTVIHGTADDVVPLDEAREYAEKFFTDLKYIVVDDGHRLQEAFK
                     ELDWEEILN"
     gene            complement(1450..2196)
                     /locus_tag="PKI33_07985"
     CDS             complement(1450..2196)
                     /locus_tag="PKI33_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36986.1"
                     /translation="MEWTGFLGFFGFCIAGLAGPLSIIVLYFVIRNIWKAHAYRRDLT
                     LHGVCAPAVVLSAWNNYSSGGGKHSAANTAHVTFEVEVMPPGQAPFRAKFKEQLSIRN
                     GEWFVIGPRKEEVGKKIWVAYDPKDPKRMVIDHFDSDHEFMLKRRAFEKQDHELQNTR
                     KTGEDANAEILEIEDMKLSNIIENGFQSVMRLKLRVEPRSGTPYETETVGMFSNTGLH
                     KYTVGKKMTVKIDRADKYKVALVGPIQQEA"
     gene            2326..>2978
                     /locus_tag="PKI33_07990"
     CDS             2326..>2978
                     /locus_tag="PKI33_07990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNM36987.1"
                     /translation="MKQHIYWHTTVQMPDPSTGSGQALPPIPSKADVAIIGGGYTGLS
                     AARTLAKQGVSVVVLEAETIGWGASSRNGGMTLTGLKPAMQTVIKNYGRELAKELFQC
                     SLDSVNIVEQIVKEEIIDCGFSRYGHLLTANKPKHYESLKGEVDFMAKEFNHNVRLVS
                     PKDLRSEIGTDVYHGALVDEVSGGLNPAQYVTGLAGAAARAGATLCARARVNKLERR"
BASE COUNT          795 a          814 c          663 g          706 t
ORIGIN      
        1 tccgtaaata tcgatgcctg caatagacct aacccgcctc cgcaaacaag cgaatcgtct
       61 cgcagatttt ttctttctgc cagatgagtt catgaagcat ctgcgtgaga tattggagtt
      121 ctacgttaat tacacgctcc gtacagtgga gaatgtcgcg ccgggctcga acctcaaaac
      181 ctaccgcaca cctcccgctg tattaactca aattcaaaac gaactgcgcc tcaaagcgga
      241 agagaatccg catttcgcgc tcgaacttgc ggatattctt tgggacgaag gcgcgctcga
      301 aactcgtctg ctcgccgcct acttgctagg acgtatcccg ccgcaggagg aacgcctgct
      361 cccccgcatc acagcatgga cacaacagat ccgcgacccg gaagtgcgcg tggcgttgct
      421 caccaccagt ctcacacgca tgcgcaagga aacaccgaat caattccttg ccctcgtgcg
      481 tgagtactta caccccgaac gctcccgttc ctggtcaaat ggcattcaag cgctcatccc
      541 catgatcaca gacagcgact ttgaaaacct gccagccatc ttcgacctcg tcgaaccgat
      601 cattgaagaa gcgccatcca ccctgcaata cgatctcacc gaccttatcg tcacgctcta
      661 tcgtgcctct gccagtgaaa ccatcttcat gctcaagcac attctcagca ccaccgagaa
      721 tcaaatgacc gctgtcacct tgcggcgcat ctcgccagat ttcccgccgc cattgcaaaa
      781 agaactgcgc gacctgctcc gcccgcagcc acttactctc atcaaacctg ccgaacccga
      841 tgacttcatc gacgagagcg caccacccga agaaaaagcc aaacctgcgc gcaagccgag
      901 aaaaaagaaa atggacaatt cccgcatcat ctacctacac ggctcagaca gcaacagcca
      961 aagcggcaaa gcccgtcagt tcgcagagaa attccccggc atggtcaccc ccgacttcac
     1021 cggcgatttc gatgagcgca tggcacaact caaaaagatc atcggtcgca aaaaaggctg
     1081 gacgatcatc ggctcatcac tgggcggatt aatgggaacg gtcttcacct gccaaaaacc
     1141 gactcacgtc cgcaagttga ttctgcttgc tccggctttg ctcaaagacc ccttcggctc
     1201 ttaccttaat ctcgagtctg tttctgttcc cacaaccgtt atccacggga cggcagatga
     1261 cgtcgttccg ctcgatgagg cgcgcgaata cgccgagaaa ttcttcaccg acttgaaata
     1321 catcgtcgtg gacgatggtc accgcttgca agaggcgttt aaagaattag attgggaaga
     1381 gatattgaat taaatcaaac accgccattt attttggcgg tgtttgattt aataggcgca
     1441 atatagccct tatgcttcct gctgtattgg acccaccaga gcaaccttgt atttatctgc
     1501 tcggtcaatt ttcacagtca tctttttacc gacagtgtat ttatgtaacc ccgtattact
     1561 aaacatccct actgtttctg tttcgtaagg cgtcccgctt ctgggttcaa cgcgcaactt
     1621 taagcgcatt acactttgga aaccattctc aataatattg gataacttca tatcttcaat
     1681 ctcaaggatc tcagcatttg catcttcacc agtcttgcga gtgttctgca attcatggtc
     1741 ttgtttttca aatgctcgac gcttcaacat aaactcgtgg tcagagtcaa aatggtcaat
     1801 caccatccgc tttggatctt tagggtcata tgccacccag attttcttgc ccacttcttc
     1861 tttgcgaggt cctataacga accattcacc atttcgaata gagagctgct ccttaaattt
     1921 agctctaaac ggagcttgcc caggaggcat cacttcaact tcaaaagtaa catgggctgt
     1981 attagccgca ctatgttttc cgccgccaga tgaataattg ttccatgctg aaagaactac
     2041 tgctggggca cagactccat gcaaggtcaa gtcacgacga tatgcatgtg ctttccaaat
     2101 attcctaatt acaaagtaga gcacaataat tgataaagga cctgccaatc cagcaatgca
     2161 aaaaccaaaa aagccaagaa aacccgtcca ctccatgtta acctctattt atttgaatga
     2221 aaatttatag aacgactaat aatattagga gtgataatat ctgattaagg tgacttaaaa
     2281 tatggcacaa ttgtcctatt ccttaacaca caacggagcc gactcatgaa acaacacatc
     2341 tactggcata ccaccgtcca aatgcctgac ccttcgacag gctcagggca ggctcttccc
     2401 cctatcccct ccaaagcgga cgtggcgatc atcggcggcg gatacacggg tttgagcgcg
     2461 gcacgaacct tggcaaaaca aggtgtgagt gtggtcgtac ttgaagcaga gacgatcggc
     2521 tggggtgcca gctctcgcaa cggcggcatg accctcacag gactaaagcc tgccatgcag
     2581 acggttatca aaaattatgg gcgggaattg gcaaaggaat tatttcagtg ttcactggat
     2641 tcggtcaata ttgttgaaca gattgtcaaa gaagagatta ttgactgtgg gttttcgaga
     2701 tatggacact tacttacggc aaacaagccc aagcattacg agtccctgaa aggcgaagtt
     2761 gattttatgg cgaaggagtt caatcacaac gtgcggttgg tttcgccaaa ggatttacgc
     2821 tctgagatcg gtacggatgt gtatcacggc gccttggtgg atgaagtcag tggcgggttg
     2881 aacccggctc aatatgtgac ggggttagcg ggtgcggcag ccagggcggg ggcaacgctt
     2941 tgcgcgcgag cgcgggtcaa caagcttgag cggagaga
//
LOCUS       DAOREF010000339         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22377_length_2972_cov_4.913267, whole genome shotgun sequence.
ACCESSION   DAOREF010000339 DAOREF010000000
VERSION     DAOREF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22377_length_2972_cov_4.913267"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..721)
                     /gene="def"
                     /locus_tag="PKI33_07995"
     CDS             complement(188..721)
                     /gene="def"
                     /locus_tag="PKI33_07995"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNM36988.1"
                     /translation="MAIRQIVHLPEPILRKKAKPVTKFDKDLQTLIDDMIETMREAPG
                     VGLAAPQINLPMQLAVVEYAEGEDEEREEGAPEPKKKLYVLINPEIIKVSEEKVLGIE
                     GCLSIPGLVGEVERHEAIQVKALNRHGDPVKIKVDGWMARIFQHEIDHLNGELFTDKA
                     VRVWKPSEEQDEETPLD"
     gene            complement(835..2703)
                     /locus_tag="PKI33_08000"
     CDS             complement(835..2703)
                     /locus_tag="PKI33_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNM36989.1"
                     /translation="MIEFKQIVIFLDKMHLFGGLDQNQLSGIANKLVEKEVPAGKEIF
                     KRGEKPDGFYMIFKGKVRVTVPDAEKGERQVAVLYGGDYFGEEALFENRNRSATVRAV
                     EDTTILFLSRQGFDGLLKQYETLKPNFQVSIKSRKLARSTRFKWLGKNEVIYFIARRH
                     KIRLFQALVTPFFSLLGALALIALGFFVSSVIPAAVGVVLVIGVLGWGVWNAIDWSND
                     YYIVTNQRVVWLEKIIFLHDSREEAPLGTILSVGVETDALGRILKYGNVIVRTFVGKL
                     EFEYVDHPQQAADMIREYWERTKAITTRTQMDVMKDTIKQKLGLPLDKRPQVELSPVV
                     PMDEEQVLKTPLWMLALQNLFQLRLEEGGKIIYHKHWIILLQQGWRPFGLFFLGIILF
                     IWRMVVLYNSPDKAFITFNGGFSFKPDTISVVIPLLLLPVAGWVWYEYTDWKNDTFMV
                     TQDEIFDIDRKPFGTEERRSAQIENILSTSYKRKGFIAYMFNYGTVNITVGGTQMAFE
                     DVMDPAAVQADINRRRAQRIAKKNEDAGSADRERFATWIAAYHQHIGEFNKPITPPAN
                     ANVSNTNPFAAVSKEKDLDDLPIKDDDDFDMDDFGDDDFDGDDDFGGDGDGGGGDN"
     gene            complement(2717..>2972)
                     /locus_tag="PKI33_08005"
     CDS             complement(2717..>2972)
                     /locus_tag="PKI33_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36990.1"
                     /translation="RLPNVLHDADCSVANLFDAQTTPHVFVIDRDGILRYRGSVDDVT
                     FRQRKPTRFFLDEAVESLLSGQFPSLTESPAYGCAIVREV"
BASE COUNT          732 a          757 c          719 g          764 t
ORIGIN      
        1 ttggaaggag gtgaatgccg ctagaaagta aatcgcttcg atgacagagg ttttgccctg
       61 cgcattttgc cctaccaaca caacagcccg ccgtggaagg tcagcatcca ggcgggtgag
      121 actgcgaaag ttggtgaggg agagatgttt gagatacata cagctttcag ctgctggcga
      181 atcgagatta atccagtggt gtttcttcgt cttgttcttc gctaggtttc caaacgcgga
      241 cggctttatc ggtaaaaagc tcgccattga gatggtcaat ctcgtgctgg aagatgcgtg
      301 ccatccaacc atctactttg attttgacag ggtcaccgtg gcggttgagc gccttaacct
      361 gaatcgcttc gtggcgctcc acttcgccga ccaagccggg aatggagaga caaccttcaa
      421 tgcccaagac cttttcttca gagactttga tgatctcagg gttgatgagc acatacagct
      481 ttttcttcgg ttcgggcgcg ccttcttcgc gttcttcatc ttcgccttcg gcgtattcca
      541 ccacagctaa ttgcatgggc aggttgatct gcggcgcagc gagacctacc cccggcgcct
      601 cgcgcatggt ttcgatcatg tcgtcgatga gagtttgaag gtctttatcg aattttgtaa
      661 cgggttttgc cttcttccgt aaaatgggtt cgggcaggtg aacgatttgt cgtattgcca
      721 tacttggtct tttccttttt gtttcagcgt acaggtgtaa gggtgaccct tacgattttg
      781 tttttcaata gggcggcaat gatacttgga ttgtcttttc cccgctagcc tacgttagtt
      841 atctccaccg ccgccgtccc cgtcaccgcc aaaatcatca tcaccatcaa aatcatcgtc
      901 gccgaagtcg tccatatcga aatcgtcatc atctttgatc gggaggtcat ccagatcttt
      961 ttcttttgag acggcagcaa acggattcgt gttgctaaca ttggcattag caggcggggt
     1021 gatcggcttg ttgaactcac caatatgttg atgataggct gcgatccaag tggcgaaacg
     1081 ttcacggtcg gcactgccgg catcttcatt tttcttggca atgcgttgag cccgccgacg
     1141 gttaatgtcc gcttgcacgg cagcagggtc catcacatct tcaaacgcca tttgagtacc
     1201 gcctacggtg atgttaactg tcccgtaatt gaacatgtag gcaataaacc ccttgcgctt
     1261 gtaagatgtg cttaagatgt tctcgatctg agcagagcgg cgttcttctg tgccgaaagg
     1321 tttgcggtca atatcgaata tctcgtcctg ggtgaccatg aaggtatcgt tcttccagtc
     1381 ggtgtattca taccaaaccc aacctgctac aggtaataac aaaagtggaa tgaccacaga
     1441 gatcgtatcg ggcttgaagg aaaagccgcc attgaaagta ataaaggctt tgtcgggcga
     1501 gttatacaac accaccatac gccaaataaa aagaatgatg ccaaggaaaa agagcccgaa
     1561 tggacgccag ccttgctgca acaaaatgat ccaatgcttg tgatagatga ttttgccacc
     1621 ctcttcaaga cgcaattgaa agaggttctg aagtgccaac atccacaatg gcgttttgag
     1681 cacctgttct tcatccatgg gcactacagg agaaagctcc acttgcggac gtttatccaa
     1741 cggcaagcct agcttctgct tgatggtgtc tttcatgaca tccatttgag tgcgggtggt
     1801 gatggctttt gtacgttccc aatactcacg gatcatatcc gcggcttgct ggggatgatc
     1861 gacgtattca aattccagct tgcccacaaa ggttcgcacg atcacattgc catatttcag
     1921 aatgcgtccc agtgcatctg tctcaacacc gacggataag atagttccta acggcgcttc
     1981 ttcgcggcta tcatgcaaga agatgatttt ctccaaccac acaacacgtt gattggtgac
     2041 gatgtagtaa tcgttgctcc agtcgatggc attccacact ccccagccta aaacgccaat
     2101 caccagcacc actcccaccg ccgccgggat gaccgaactt acgaagaagc ctaaagcgat
     2161 taatgccagc gcgccaagca aagaaaagaa tggcgtgacc aatgcctgaa agaggcgaat
     2221 tttatgtctg cgagcaatga agtaaatgac ttcgttcttc cccaaccact taaagcgtgt
     2281 tgaacgcgca agtttgcggc tcttaatgga aacttgaaaa tttggtttaa gggtttcgta
     2341 ctgtttaagc agcccgtcaa aaccctgacg tgaaagaaat agaatagttg tatcttccac
     2401 cgcccgaacc gtggcagacc gattgcggtt ttcaaacagc gcttcttcac cgaagtagtc
     2461 gccgccgtat agcactgcta cttggcgttc acctttttca gcatctggca cggtcacacg
     2521 cactttcccc ttaaagatca tataaaagcc atctggcttt tcgccccgct taaaaatttc
     2581 cttcccggcg ggaacttctt tttcaacaag cttgttggca ataccagaaa gctgattctg
     2641 gtccaacccg ccgaagaggt gcattttatc gaggaaaatg acgatttgtt tgaattcgat
     2701 cacgagcgtg attctatcaa acttcacgca caatggcgca gccgtaagca ggagattccg
     2761 tcagcgacgg gaactgccca gagagcaaag actcaaccgc ctcgtccaga aagaagcgcg
     2821 tgggctttcg ctggcggaag gtcacgtcat ccaccgagcc acggtagcgc aggattccat
     2881 ccctatcgat gacgaagaca tgcggagtgg tctgcgcgtc aaacaaattt gcgaccgaac
     2941 aatccgcatc atgcaagacg tttggcaggc gg
//
LOCUS       DAOREF010000340         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22420_length_2968_cov_4.715070, whole genome shotgun sequence.
ACCESSION   DAOREF010000340 DAOREF010000000
VERSION     DAOREF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22420_length_2968_cov_4.715070"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..907)
                     /locus_tag="PKI33_08010"
     CDS             complement(98..907)
                     /locus_tag="PKI33_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36991.1"
                     /translation="MSRQKAFLSLCLVLVLALACNLPSATPTETAAANPEDLELTITA
                     LASGQGNQPSDSPAQDTATPEFTPITGPTSTPTVPQVTVSQNTNCRVGPGVVYDLIDA
                     VLIGQTAEVVGKNSGVPNYWVVKRLNGSGTCWLWGQYATVSGNTANLPEYPVPPTPTP
                     SPTPTFTPTPTLAPPAPVNNVSVVKNCIPLVFPTTYTYTGILNWEDKSNNENGFNIYF
                     NGALFASIPANSNSYPLPALPFAAGTPVKWGVEAFNSAGKAAIKDVIFACP"
     gene            complement(1021..1809)
                     /locus_tag="PKI33_08015"
     CDS             complement(1021..1809)
                     /locus_tag="PKI33_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36992.1"
                     /translation="MFRRKITLFVCIFLVGALSACNLPSSAPTETETSELDLALTITA
                     LASSPNAPQETSTPEFTATAGPTSTPSVPQVSVSQNTNCRVGPGIVYDLIDSLLIGQT
                     AEIVGKNSGVPNYWVIKRINGSGTCWLWGEYATVTGNTANLPEYPVPPTPTPSPTPTF
                     TPTATPSAPAAVENVTMTMSTCGGVAPYQHGGTLAWEDKSNNEDGFNIYVNNVLIASI
                     PANSTSYNVAPAGSFAAGIASKFEVEAFNGVGKAAKKSVAKGCP"
     gene            complement(1926..2666)
                     /locus_tag="PKI33_08020"
     CDS             complement(1926..2666)
                     /locus_tag="PKI33_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNM36993.1"
                     /translation="MTRIAVISDIHGNILALEKVVQDIKRRGVDQVVNLGDHVSGPLW
                     AKETIQFLMEQSWIQIRGNHDRQLVSQAPETHGASDKSAFQQLNENELDWLRKLPPQA
                     ELLGEILFVHGTPKSDNTYLLETIAHGSNRIATRNEIKERIGDSQHKLILCGHTHIPR
                     VVAISEQTTIINPGSVGLQAYDDTSPEYHIMQTGSPHARYVILEKVDINWVVNLLHVE
                     YDHNSAAAHAHKNGRIEWEYALRTGLMP"
     gene            complement(2663..>2968)
                     /locus_tag="PKI33_08025"
     CDS             complement(2663..>2968)
                     /locus_tag="PKI33_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNM36994.1"
                     /translation="IPAGAFHPVPNVDSAILRIDIYDQPLISAEMLSTFFKLIKAGFS
                     QKRKTLRNSLSSGLHIKPQDAETLLNKSNIDPMRRAETLSIAEWKGVCETMAQGNSK"
BASE COUNT          779 a          606 c          704 g          879 t
ORIGIN      
        1 tttgcggaac atgaaatgtt gacatcgttt ctgattatac aaaataaaaa gccttacaga
       61 aaaacttctg caaggctttt taatacttat caactttcta tgggcaggcg aatattacat
      121 ctttaattgc agccttgcct gcagagttga aggcttctac accccatttt acaggcgtcc
      181 cagcagcaaa aggtaacgcc ggcaaaggat aactgttgct atttgctgga atactggcga
      241 acaaagcacc attgaaatag atattgaacc cgttctcgtt attcgattta tcttcccaat
      301 taagtattcc agtataggtg tacgttgttg gaaataccaa tggaatacaa ttctttacaa
      361 cggaaacatt atttactggc gctggtggtg caagtgttgg tgtaggtgta aatgtcggag
      421 ttggagaagg tgtcggcgtg ggcggaaccg gatattccgg caaattcgct gtattaccgc
      481 taacggtggc atattgtccc cagagccaac aagttccaga cccgttcagg cgtttgacca
      541 cccaataatt cgggacgccg gagtttttac caactacttc tgccgtttgc ccaataagca
      601 cagcatcgat taggtcatat acaacccccg gaccaacacg acaatttgta ttttggctga
      661 ccgtgacttg tggcacagtt ggcgtagacg ttggacctgt tataggggta aattcgggtg
      721 tggcggtatc ttgagcaggg gagtcgctgg gttgatttcc ctgtccgctt gctaaggctg
      781 taatggttaa ttctaagtct tcagggttgg ctgccgcagt ttcggttggg gtagcacttg
      841 gcaagttaca tgcaagtgca aggactaaga ctaaacacaa agacaaaaaa gctttttgac
      901 gtgacataaa acctctcttt catataccta ttattgcaca cctaagaaaa tttattgtaa
      961 aactagcacg ttaaagtata aatctggcag ggatttccct gccagattta tgagaaaact
     1021 ttatgggcaa ccctttgcaa cactcttttt agctgccttt ccaacaccat tgaaggcttc
     1081 cacttcgaat tttgaagcaa ttcctgcggc aaacgagccg gctggagcaa cattgtacga
     1141 agtactattc gctgggatgc tggcaattaa gacattgttt acataaatat tgaaaccgtc
     1201 ttcattgttg gatttatctt cccatgctag agtaccacca tgttgatagg gagctactcc
     1261 accacaagtt gacatggtca ttgttacgtt ttcaactgcg gcaggcgctg aaggtgttgc
     1321 tgtaggtgta aatgtcggag ttggagaagg tgtcggtgtg ggaggaaccg gatattctgg
     1381 cagattcgca gtgtttcccg ttacggtggc gtactcgccc cagagccaac aagttccaga
     1441 tccattgatg cgtttgatga cccaataatt gggaacgcca gaatttttac caacgatttc
     1501 tgctgtttgc ccaatgagca ggctatcaat taaatcatag acaatgccag gacctacacg
     1561 gcaatttgta ttttgactaa ccgatacctg tggaacagag ggtgtagatg tgggacccgc
     1621 tgtggcagta aactctggcg tggaggtttc ctgcggtgca ttcggactgc tggcaagagc
     1681 cgtaatcgtg agtgcaagat cgagttctga agtttcagtc tcagtgggag cactgcttgg
     1741 aaggttacaa gcagaaagag caccaactaa gaatatacaa acgaataaag ttatttttct
     1801 gcgaaacata attctctcct ttataagaaa ctattatggc acaactttga tatatatcaa
     1861 taaaagggct tgtgagatta gtactagtgt gcaaagggac gttggtacta gtactagctt
     1921 ttgttctagg gcattaaacc ggttcttaac gcatattccc attcaatccg cccatttttg
     1981 tgagcgtgtg ctgcagcaga gttatggtca tactcaacat gaagtagatt cacgacccaa
     2041 ttaatgtcaa ccttttcaag aattacatac cttgcatgtg gcgaacctgt ttgcatgatg
     2101 tgatattctg ggctggtatc atcgtatgct tgaagcccca cactaccggg gtttatgatc
     2161 gtagtctgtt ctgagattgc aactactctg ggaatatgag tgtgtccgca gagaataagt
     2221 ttgtgttgag aatcaccaat tcgttctttt atctcattcc gtgtagcaat tctattgctg
     2281 ccatgagcga tggtttcaag taggtaggtg ttatcacttt ttggtgttcc atggacgaat
     2341 agaatttcgc ctaacaactc tgcttgagga ggtagtttgc gaagccaatc taattcattt
     2401 tcattcaatt gttgaaaggc agacttatca gaggctccat gtgtttctgg agcttgtgag
     2461 acgagttgtc tgtcgtggtt tccgcggatt tggatccagc tctgttccat caagaattgg
     2521 atggtttcct tcgcccaaag cggacctgat acatggtcac ccaagttgac gacttgatct
     2581 acacctcgtc ttttaatatc ttgaacaact ttttcaaggg caaggatgtt tccatgaata
     2641 tctgaaatga cagcgattct tgtcatttgc tattcccttg agccatggtt tcgcataccc
     2701 ctttccattc tgcgatggat aaagtctcag cccgacgcat ggggtcgata tttgatttat
     2761 taagtaaagt ctctgcgtct tgtggtttga tatgtagccc agaagagagt gaatttcgta
     2821 acgttttgcg cttttgacta aagccggctt tgatgagttt gaagaatgtg cttagcattt
     2881 ctgcagagat aagcggttgg tcgtagatat caatgcgcag aatagcagag tctacattgg
     2941 ggacgggatg aaatgcccca gcggggat
//
LOCUS       DAOREF010000341         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22421_length_2968_cov_4.063852, whole genome shotgun sequence.
ACCESSION   DAOREF010000341 DAOREF010000000
VERSION     DAOREF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22421_length_2968_cov_4.063852"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..2546)
                     /locus_tag="PKI33_08030"
     CDS             complement(204..2546)
                     /locus_tag="PKI33_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNM36995.1"
                     /translation="MDKNRALQRLLNGKATNKEIEELKQAVASGEISIGGDMNRSVVI
                     IGSGNKVELTAEALGILKPENTTQEPVTGESPYMGLRYFDTQDADLFYGREALTGELL
                     SRVKNEPFLAIVGASGSGKSSVARAGLIPAWKRENERGAIHIITPTAHPLESLAASLT
                     RDSESVTATSTLMDDMSKEPRSLRLYVKKALGEKKLLLLIDQFEETFTLCKEPEERKA
                     FIENLLSLAEADGAARVVITLRADFYHHCFEYEGLRLTLEKHQANLGAMTQDELREAI
                     LKPAETNGWDFQPGLADLILQDIGSEPGALPLLSHALLETWKRRQGCTLTLQGYHEAG
                     GVKKAITQTAESVYAHLTSAEQTIARRIFLDLTELGENAQDTRRRVKLENLVQNKEQD
                     AVTKVLKTLTDARLVTTEEDSAEVAHEALIREWVTLSKWLDENRESLNLYRHMQKSAR
                     EWDRRGKEASELYRGARLKQLQGWVKDHGDSLSPLECEFVNTSQKVKKRERLRGAIFV
                     CISIVVLFCSVVLAVNGKFNQYFFIPVDMKDYWVTIPAGEFLMGSSDKQVIESLKTCS
                     ECNFPNEYPQHRVYVNTYEIGKYEVTNRQYAECVRAGVCAEGIFSYEYYKDLDSSPVI
                     NVNWYGAKTYCEWVGGRLPTEAEWEKAARGGLEYKLYPWGDEVPVCTKGKSNGANFSG
                     GIGCPNDVTAVGSFSANRYGVFDMAGNVSEWTSSLYMAYPNDSNDGREDMNSSADRVI
                     RGGSWVDSFDSVRSASRSWNNPIISNLFIGFRCARTPEGQ"
     gene            complement(2658..>2968)
                     /locus_tag="PKI33_08035"
     CDS             complement(2658..>2968)
                     /locus_tag="PKI33_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNM36996.1"
                     /translation="WIFQFWAKSGSPQESMDKLWNMSPVKYLGETKTPTLIIHNEHDH
                     RCPIEQGEQAFVALKRAGVETEFVRFPEEPHGLSRVGRTDRRIARLNHILRWMDKYLK
                     "
BASE COUNT          703 a          782 c          641 g          842 t
ORIGIN      
        1 atcctgcccg atgtagtttg aaatttttga aacgctgacg tgttcggtca tgtagggggt
       61 tcctctgaat aaatgatttt ggtaattata ttacaggttg atggtcattc cgacaaagac
      121 cttaccgatt ctataaaggg tcagatgata gtctctttta tacaacatcc acaataaagc
      181 agttattcat ccttctgcat ttatcattgc ccttctggtg tgcgagcaca acgaaagccg
      241 atgaagaggt tagagattat tgggttgttc cagctgcgac tggcagagcg tacggagtca
      301 aagctgtcca cccacgaacc gccgcgtatc acccgatcag cggatgaatt catatcttct
      361 cgaccatcgt tggagtcatt tgggtaagcc atatacaaac tgcttgtcca ttccgagaca
      421 ttgcctgcca tatcaaatac cccataccta tttgctgaaa aacttcctac agcagtgaca
      481 tcatttggac aaccaatacc gccagaaaaa ttagctccat ttgacttacc ttttgtacat
      541 accggtacct catctcccca tggatagagc ttatattcta gtcccccacg tgctgccttt
      601 tcccattctg cctcggtggg cagacgtccg cctacccatt cacaataagt cttagctcca
      661 taccaattaa cattaattac tggtgatgaa tctaaatctt tataatactc ataggaaaat
      721 atgccttcag cacagacacc agctcgcaca cactcagcat actgacgatt agtgacttcg
      781 tatttcccga tttcataggt attaacataa actcggtgtt gaggatattc attgggaaaa
      841 ttgcactctg agcatgtttt caaagattct attacttgtt tatctgaact gcccattaga
      901 aattcgccag caggaatagt aacccaatag tccttcatgt caaccggaat aaaaaaatac
      961 tgattgaatt ttccatttac cgccaagaca accgaacaaa acaacacaac aatgctaatg
     1021 cacacaaaaa tagctccacg taatcgttca cgctttttca ccttttggct tgtatttaca
     1081 aactcacatt ccagcgggct gagcgagtcg ccatggtcct tcacccagcc ctgcaattgc
     1141 ttcaaccttg cgccgcggta taactcgctg gcttctttac cccttcggtc ccattctctt
     1201 gcactttttt gcatgtgacg atacaagttc aggctctcgc gattttcatc cagccatttg
     1261 ctcaaggtta cccattcacg aatcaaggct tcgtgcgcca cttctgcgct atcttcctcg
     1321 gtggtgacta aacgtgcatc ggttaaagtc tttagcacct ttgttacagc atcttgttct
     1381 ttgttttgca ccagattttc caacttgact cggcggcgag tatcctgcgc gttttcgccc
     1441 agttcggtta gatccaaaaa tatcctacgt gcaatggttt gttcggcaga ggttaggtgg
     1501 gcatatacgc tttcagcagt ttgggtgatg gctttcttca caccgcccgc ttcgtgatag
     1561 ccctgcaagg tcagtgtgca tccctgcctg cgtttccatg tttccagcag ggcatgagaa
     1621 agcaatggca acgcgccggg ttcagaaccg atgtcttgca ggatcaaatc cgccaagccg
     1681 ggttgaaagt cccagccgtt ggtttcggca ggcttgagaa tggcttcgcg taactcatct
     1741 tgagtcatcg cgccgaggtt ggcttggtgt ttttccaacg tcaagcgcaa gccttcgtat
     1801 tcgaagcaat gatgataaaa gtccgcgcgt aaggtgatga ccacccgtgc agcgccgtct
     1861 gcttctgcga gggataacaa gttctcgatg aatgccttgc gttcttctgg ctctttgcag
     1921 agggtgaagg tctcttcgaa ttgatcgatc agcaataata atttcttttc ccccaaagcc
     1981 ttttttacat acaacctcaa actgcggggt tctttggaca tatcgtccat caatgtggat
     2041 gtcgccgtga ccgattccga atcgcgcgtc agactcgccg cgaggctttc caacggatga
     2101 gccgtgggtg tgatgatgtg gatcgctccg cgctcgttct cccgcttcca tgcagggatc
     2161 aatcccgccc gtgccacaga cgattttccg ctgcccgatg cgcccacaat ggcaaggaag
     2221 ggttcgtttt taacacgcga taacagttca ccggtcaatg cctcgcgccc atagaacaag
     2281 tccgcatcct gggtatcgaa gtagcgcaag cccatatagg gcgactcgcc cgtgacgggt
     2341 tcttgagtag tgttttcagg ttttaaaatg ccaagcgctt ctgctgtcag ttcaacttta
     2401 ttcccacttc cgataattac aaccgagcgg ttcatatccc cgccgattga aatttcaccg
     2461 ctggcaacag cctgctttaa ctcttcaatt tctttatttg tggctttgcc atttaatagt
     2521 ctttgcaggg ccctgttttt atccatgaat caccttgtta gtcagattat accgccactc
     2581 taattcaaaa aggtgacagt catcttttgc gacatcgccc cgtagcgggc aagatgactg
     2641 tcacctctct ttcaaatcta cttcaaatac ttatccatcc acctcaagat gtgattcaac
     2701 cgcgcaatgc gccgatctgt tcttccgacg cgtgaaagcc catgcggttc ttcagggaag
     2761 cgcacgaact ccgtctccac gccagcgcgt ttgagtgcta cgaaggcttg ctcgccttgc
     2821 tcgatgggac agcgatggtc atgctcgttg tggatgatga gcgtgggcgt cttggtttcg
     2881 cccaaatatt tcacgggcga catgttccag agtttatcca tgctctcttg cggcgagcca
     2941 gattttgccc agaactggaa gatccagt
//
LOCUS       DAOREF010000342         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22450_length_2965_cov_5.032990, whole genome shotgun sequence.
ACCESSION   DAOREF010000342 DAOREF010000000
VERSION     DAOREF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22450_length_2965_cov_5.032990"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKI33_08040"
     CDS             <1..475
                     /locus_tag="PKI33_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HNM36997.1"
                     /translation="LGMTHLWEKIRVQGGAYGAFAQFDDATGVFTYLSYRDPNVDNTI
                     ASYDSSAAFLKNLDASRLNDNELKKSIISAIGNLDSYQLPDAKGYTSMMRFITGRSDE
                     IRQKYRDQVLSTNGEDFIAFGEVLEKVAQSDAVVVLGSQSALEGSKAGLKVMKVA"
     gene            515..1012
                     /locus_tag="PKI33_08045"
     CDS             515..1012
                     /locus_tag="PKI33_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM36998.1"
                     /translation="MAGLEEQLVSELWARDVRFILGTAPNHPPMLSPVELIKGLAVSV
                     ETRLQLSLIPVFLRHPEFSIYAESVAKNLPLEKQIILKCFYCAAVWLECKHLSQNNLP
                     DLFSKDLGLLPTSDPDNNLLLLANRQKELSSSRINWAATYQHAADIWLKDLELRKMKN
                     GKRNS"
     gene            993..1505
                     /locus_tag="PKI33_08050"
     CDS             993..1505
                     /locus_tag="PKI33_08050"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HNM36999.1"
                     /translation="MEKETLESILKEFGKRVPPGSQLHLLGGGALTLLGSPRPSLDID
                     FVGDDVHPNELHRTILSIAKEMDLFVDAVPIDRFVPLPEGHENRAIHIGQFENLEIFV
                     IDPYSIALSKVDRGSFTDYDDILFLIQKRYIDMSKLEEIIKEAVAKAGKYDLHPEILS
                     HFEELKSRLS"
     gene            1640..>2965
                     /locus_tag="PKI33_08055"
     CDS             1640..>2965
                     /locus_tag="PKI33_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNM37000.1"
                     /translation="MNITLKINGIEHTIEAPASTTLLAALRGLGFHGVKFGDEQGLTG
                     ADTVLLDGKPINAGSMLAAQAEGHAVATIEALGEHPDLGWRKTDGLHPLQKAFVESGA
                     IQCGYCTPAQILAAKSLLEKNPNPSEAEVREAISGVLCRCTGYLKPVQAVLKAAAVLR
                     GDEPFDVTSIHWDFGTPKTDSPSSDSSASPSTSAVLTRPKVVVTPETEKWQTVGKPEK
                     KVDAIKLVQGKPAFAADMDMRGMLHAKVLHSPHAHAQIKNINASKALALKGVVCVLTW
                     KDLPRVIYSTAGQSDPIPGPLDAFSLDKKVRFVGDRVAFVAAETAEIAEQALALIDVE
                     YEILPAIIDSRESMKQGAVKIHDEPEYVNFADSNADKNLAAHIRIDIGDVEKGFAEAD
                     EIFEAEYEVPKVQHGHIEPHVCVTYWDEDDRLVIRTSTQVPFHVRRMMAP"
BASE COUNT          790 a          726 c          730 g          719 t
ORIGIN      
        1 cctcggcatg actcacttgt gggaaaagat ccgcgtgcaa ggcggtgcgt atggcgcctt
       61 tgcccaattt gatgacgcga ccggtgtgtt cacctacctt tcctaccgcg acccgaacgt
      121 ggataacacc atcgccagtt acgacagttc cgccgcgttt ttgaaaaacc tcgacgcctc
      181 acgcctgaac gacaacgaat tgaagaaatc catcatctcc gccatcggta atttagactc
      241 gtatcaactg cccgacgcaa agggctacac ttcaatgatg cgcttcatca ccggtcgcag
      301 tgatgaaatc cgccagaagt atcgtgatca ggtactctcc actaacggcg aagatttcat
      361 cgcattcggc gaagttttgg agaaagtagc gcaatcggat gctgtcgtgg tgctggggtc
      421 acagtccgcg ctggaggggt caaaggcagg gctgaaggtg atgaaggtgg cgtagaagag
      481 cagagattag agattggata attagagatt agttatggca ggtttggaag agcaattagt
      541 gtcagaactt tgggcaaggg atgtgcggtt cattcttgga actgcgccaa atcacccgcc
      601 catgctttcg cctgtggaat tgataaaagg gcttgcagta agcgttgaaa cacgtttgca
      661 gttgagtctg attccagtgt tccttcgcca tcccgaattc tctatttacg ccgaatctgt
      721 tgcaaaaaat ctacctttgg aaaagcagat aatactaaag tgtttttatt gcgcggcagt
      781 atggctcgag tgcaaacatc tttcacaaaa caatctgcct gacttatttt caaaagattt
      841 aggattatta cctacatctg acccagataa caatctgctt ttgcttgcaa accgacaaaa
      901 agagttaagt agttctcgaa tcaattgggc tgccacctat caacatgcag cggatatttg
      961 gttaaaagac cttgagttgc gaaagatgaa aaatggaaaa agaaactctt gaaagcattc
     1021 taaaagagtt cgggaagcgc gttccacctg gttcgcaatt gcaccttctt ggcggcggag
     1081 cgttaaccct actaggaagt ccgcgacctt ctcttgacat tgattttgta ggcgatgatg
     1141 tccatccgaa tgagttacat cgtacaattc tgagcattgc caaagaaatg gatttgtttg
     1201 ttgatgcagt tcccattgac cgattcgtcc cattaccaga aggtcacgaa aaccgcgcca
     1261 ttcatatagg tcaatttgaa aatttggaaa tatttgttat tgatccatac agcattgcgt
     1321 tgagcaaagt agaccgaggc tcatttaccg attatgacga tatcctcttt ttgattcaaa
     1381 aaagatatat tgatatgagc aaattggaag aaatcatcaa agaagcggtt gccaaagctg
     1441 gcaaatatga ccttcaccct gaaatcctta gccattttga agaactaaaa tcaagactct
     1501 cttaatctct aaagtggcat agacaaactt gaaaaacact tccatatttt tagtacaata
     1561 agagggcaag gatattcctt gccctcttat ttcaatctcc gtaatacata agccattgca
     1621 taactctcaa ggagttttca tgaacatcac actcaagatc aatggaattg aacacaccat
     1681 cgaagcgcct gcttcgacta ccctgcttgc cgccctgcgc gggctgggtt ttcacggcgt
     1741 gaagtttggc gacgagcaag gtctcaccgg cgcggatact gtgctgctgg atggcaaacc
     1801 catcaacgca ggttccatgt tggcggcgca ggcggagggc catgctgtgg cgacgatcga
     1861 agccctgggc gagcacccag acctcggctg gagaaagacc gatggtcttc atccgctgca
     1921 aaaggctttt gttgaatccg gcgcgatcca atgcgggtac tgcacaccgg cgcagatcct
     1981 tgctgcaaaa agtttgcttg agaaaaaccc gaaccccagt gaagccgaag tgcgtgaagc
     2041 gatctctggt gtattgtgcc gctgcactgg ttatctcaaa cccgtgcaag ccgtgttgaa
     2101 agccgcagca gttttgcggg gtgacgaacc ctttgacgtg acctccatcc attgggattt
     2161 tggcacaccg aaaacggatt ctccttccag tgactcttcc gcttctccct cgacctcagc
     2221 tgtgctcacc cgacctaaag tggtggttac gccggagacg gagaaatggc aaaccgtggg
     2281 caagcccgaa aagaaagtgg acgccatcaa gttggtccag ggcaaacctg ccttcgccgc
     2341 cgacatggac atgcgcggca tgttacatgc caaagtgctg cactcgcccc atgcccatgc
     2401 acaaatcaaa aacattaacg caagcaaagc gctggcattg aaaggtgtcg tttgtgtact
     2461 aacctggaaa gacctgccac gggtgatcta ctccactgcc gggcaatccg acccaatccc
     2521 cggtccgctg gatgcattct cactggacaa aaaggtccgt ttcgtcggtg accgcgtggc
     2581 attcgttgcc gcagagaccg cagaaattgc agagcaggct ttggcgctga tcgatgtgga
     2641 atatgaaatt ctgcctgcca ttatcgactc gcgtgagtcg atgaaacagg gcgcagtcaa
     2701 aattcacgac gagccggagt atgtcaactt tgccgactcg aatgccgata aaaatctcgc
     2761 ggcgcacatc cgcattgata ttggcgatgt ggaaaaaggg ttcgccgaag ccgatgaaat
     2821 ctttgaagcc gaatatgaag ttcccaaggt gcagcatggg catattgaac cgcatgtatg
     2881 cgtcacctat tgggatgaag atgatcgtct ggtcattcgc acttccacac aggttccgtt
     2941 ccatgtgcgc cgtatgatgg caccc
//
LOCUS       DAOREF010000343         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22500_length_2962_cov_2.556588, whole genome shotgun sequence.
ACCESSION   DAOREF010000343 DAOREF010000000
VERSION     DAOREF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22500_length_2962_cov_2.556588"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKI33_08060"
     CDS             complement(<1..1006)
                     /locus_tag="PKI33_08060"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNM37001.1"
                     /translation="MSSRPHIRNRFVLIGDLALTIVSVLGSFALRLDVSELPFYFPAA
                     LVMCAVTLLVKIPTYYFFGLYRRLWVYASTNELRLITFAVTTASVLASGVMLLLIGFD
                     LIKPGMPRSALGIDWLISLILIGGSRFVLRILAEQSSAQANANTRHALILGAGDAGAL
                     VVRELQKTSQLNLIPVGFLDDDPAKQNHNIYGVTVIGKIDQLANTLDSKQVNEVIIAI
                     PSAPGHIIRLVNDECRKRGITSRIIPGFYELIGGKVSVNRLREVDITDLLRRDPAKID
                     DRLIGATLGGKRILVTGAGGSIGSELCRQLARWNPTELVLLGHGENSIFEALQELKND
                     F"
     gene            complement(1054..1980)
                     /locus_tag="PKI33_08065"
     CDS             complement(1054..1980)
                     /locus_tag="PKI33_08065"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM37002.1"
                     /translation="MKYLVTGGAGFIGSHISRTLLERGHSVRIFDNFSSGKRDNLKGL
                     DVEVIEGDLRDADAVAKAVQGMEIIFHEAAFVSVPESMEKPQECFDVNVTGTSILFEA
                     ARKAGVKRAVFATSAAVYGDSEAYPLSEDTPLRQLSPYATSKRVDELYGQLYTSSFNF
                     EVVALRYFNVYGPRQRPDSMYAAAVPIFIRRILDNKPITIFGDGGQSRDLVNVRDVVQ
                     ANLLAAEHPAAPGQIFNVCTGVETRLLDLLDILYEIFPNAPKHVHAEPRAGDIYRSIG
                     TPKKIMDMLGYKPQVTLAEGLKEAVEVMSNEY"
     gene            complement(1984..>2962)
                     /locus_tag="PKI33_08070"
     CDS             complement(1984..>2962)
                     /locus_tag="PKI33_08070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNM37003.1"
                     /translation="MRRVLIVGAGKAGRTLVEAYRKLNPPPFLLIGYIDDDPAKRNKS
                     YNGFAVLGDSEHLFDLIEDYRISDVVVAINGEIKGETFQTILDVQERGVEVTRMPIMY
                     EELTQRVPIEHLETDWVIRSFVDQVRVHGLYELLKRTMDILGGIVGTLIFLMFLPFIA
                     VAIILETGFPVFYSQPRLGKGAQPFTILKFRTMKQDAEMDGQPKVAEENDPRVTRIGT
                     FLRKTRLDELPQFWTVLRGEMSLVGPRAERPELVAEYQKQIPYYRARLLVKPGLTGWA
                     QINYGYVATVKETYIKLEYDLYYIKHRTLNMDFSIVLRTIGTVLRRTGR"
BASE COUNT          783 a          715 c          777 g          687 t
ORIGIN      
        1 gaaaatcgtt cttgagttct tgcaaggctt caaagatgct gttctcgcca tgcccgagca
       61 aaacgagttc ggtcggattc caacgcgcaa gctgacgaca gagttcactg ccgatcgagc
      121 cgcctgcgcc ggtgacgagg atgcgtttgc cgcccagagt agcgccaatg agacggtcat
      181 caattttggc ggggtcgcgg cgtaggaggt cggtgatgtc cacttcgcgc agacggttga
      241 cgctgacctt gccaccgatc agttcgtaaa agccggggat gatgcgtgag gtgattccgc
      301 gtttgcggca ttcatcgttg acgaggcgga tgatatgccc cggtgcggag ggaatggcga
      361 tgatgacttc gttcacctgc ttggaatcga gcgtatttgc aagttgatcg atcttaccga
      421 taacggtcac accgtagatg ttgtgattct gcttggcggg gtcatcgtcg agaaagccaa
      481 caggaatgag attaagttgg gaggtcttct gtaactcacg cacgacgagc gcacccgcgt
      541 cgcctgcgcc gaggatgagg gcgtggcgag tattggcatt ggcttgcgcc gaagattgtt
      601 cagcaaggat gcgcagaaca aaacgcgagc caccaataag gataagcgag ataagccagt
      661 caatgccgag agcggagcgc ggcatgcccg gcttgatcaa gtcaaagccg atgagcagga
      721 gcatcacgcc tgaagccagg acagaagcgg tggtaacagc aaaggtgatg aggcgtaatt
      781 cattcgtgct ggcatacacc cataaacgac gatagagacc gaagaaataa taggttggaa
      841 ttttgacaag caacgtcacc gcacacatta ccagtgcggc agggaaataa aacggtaatt
      901 cgctcacatc cagccgtaac gcaaaactgc ccaacaccga aacgatggtg agggcaaggt
      961 cgccaatcaa gacgaagcgg ttgcggatgt ggggacgaga agacatgggg aaaagaagtt
     1021 attggtaacg aggaacaaga aatgaggaaa ttcctagtac tcgttactca ttacttctac
     1081 cgcttctttc aacccttcag ccaaggtcac ttgcggtttg tagccgagca tgtccatgat
     1141 tttcttgggc gtaccgatgg agcgatagat gtcaccggcg cgtggttcgg cgtggacatg
     1201 tttgggagca ttggggaaga tttcgtagag aatgtccagc aggtcgagta gacgagtttc
     1261 aactccggtg cagacattga aaatttgccc cggtgcggcg ggatgttcgg cggcgagtag
     1321 attggcttgc actacatcgc gcacgttgac caggtcacgg ctctgaccac cgtcgccgaa
     1381 gatcgtgatc ggcttgttat ccaaaatgcg gcgaatgaag atcggtacag cggcagcgta
     1441 catcgagtcc ggtcgctggc gcggtccgta gacgttgaag tagcgtaagg cgacaacttc
     1501 aaagttgaac gatgaagtgt agagttgacc gtaaagttca tctacacgct tggaagtggc
     1561 gtagggtgat aactgtcgca agggagtatc ttccgaaagc ggataggctt ccgagtcgcc
     1621 gtacacagcg gcactggtag cgaagacagc gcgcttcacg cctgccttac gggcggcttc
     1681 aaacaaaatg gaggttcccg tcacattgac atcgaaacat tcctgcggct tctccatcga
     1741 ctcggggacg gaaacaaacg ccgcttcatg gaagataatt tccatgcctt gtacggcttt
     1801 ggcaaccgca tcggcatcgc gcaagtcgcc ttcgataacc tctacatcca aacctttgag
     1861 gttatcacgc ttaccagagg agaaattatc aaagatacgg acggagtgtc ctctttccaa
     1921 caatgtacgt gaaatgtgcg agccaataaa gcccgcaccg ccagtgacta aatatttcat
     1981 aggttatctc ccagttcttc ttaatactgt tccgattgtc cttaacacaa tactaaaatc
     2041 catgtttagg gttcggtgtt taatgtagta tagatcgtac tcaagcttga tataggtctc
     2101 tttgaccgtt gcaacatatc cataattaat ctgcgcccat cctgtaagtc caggctttac
     2161 caataatctt gcacgataat acggtatctg tttctgatac tctgcaacta attcagggcg
     2221 ctcagcacgc ggaccaacta gactcatttc gccacgcagc acagtccaaa attgcggaag
     2281 ttcatcaagg cgagtctttc gaaggaaagt gccaattcgg gtaacacgtg ggtcattctc
     2341 ttcagccact ttaggttgcc cgtccatttc tgcatcttgt ttcatggtgc ggaatttaag
     2401 aatagtaaaa ggctgtgcac cttttcctaa acgcggctga gaataaaaaa cagggaaacc
     2461 tgtttcaaga ataatggcaa cagcaataaa ggggagaaac atcaaaaaga tgagcgttcc
     2521 aacaatgcca cccaaaatat ccattgtgcg ttttagcaat tcatacagtc catgcacgcg
     2581 cacttggtcg acaaatgagc gaatcaccca atcggtttca agatgttcga tcggcactcg
     2641 ttgagtcaat tcttcataca taatgggcat gcgagttact tcgacacctc gctcttgcac
     2701 atcaagaatg gtttgaaagg tttcgccttt gatctcaccg ttaatcgcca caacaacatc
     2761 tgaaatgcga taatcttcga tcaggtcgaa aagatgttcg ctatcaccaa gcacagcaaa
     2821 cccgttatat gatttattcc gttttgccgg gtcgtcatca atataaccaa tcaaaaggaa
     2881 tggcggtgga ttcagtttgc ggtacgcctc tacgagagtc cgcccggctt tgcctgcccc
     2941 tacaataagc acacggcgca tc
//
LOCUS       DAOREF010000344         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22706_length_2947_cov_3.881743, whole genome shotgun sequence.
ACCESSION   DAOREF010000344 DAOREF010000000
VERSION     DAOREF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22706_length_2947_cov_3.881743"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKI33_08075"
     CDS             <1..242
                     /locus_tag="PKI33_08075"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNM37004.1"
                     /translation="GQHATLRDDLYILLVDWQPVSSEGATFKIYVNPLVNWLWIGSLT
                     FLFGLIFAAWPDRDPADEPLRRKVSAGNRQTSAAD"
     gene            292..783
                     /locus_tag="PKI33_08080"
     CDS             292..783
                     /locus_tag="PKI33_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNM37005.1"
                     /translation="MKKFLFILTLMLVASGLFTAVAFAQDKTPTDDEVNAIAKQLYCP
                     VCENTPLDVCPTEACHQWRELIRQMLAEGKSEQQIKDYFVANYGARVLSEPPRAGFNW
                     LVYIIPPVLILVGAVFLFNAFRAWTKPKSAEAGSGQAEEAGTSSSDDEYIKRLEEELK
                     NRK"
     gene            816..1385
                     /locus_tag="PKI33_08085"
     CDS             816..1385
                     /locus_tag="PKI33_08085"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNM37006.1"
                     /translation="MTETTAPARKSVPLWVQVTIWVFLAGLLALVGFGLNRASHPMAE
                     VGDTVPDFKLVYYEGYEHNNTPEMKLSDLRGKVVLINIWASWCKPCEQEAPELEQAWQ
                     LYKDQGDVVFIGVDYVDTPEGAFGYLKKFGITFPNAPDLQSNISSILNRQMGVPETYL
                     IDRDGVLRQIKIGPFASVSEIQAFIASAE"
     gene            1395..1904
                     /locus_tag="PKI33_08090"
     CDS             1395..1904
                     /locus_tag="PKI33_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNM37007.1"
                     /translation="MELGSIFLILAVLVAVGMYLYAPFMYRSRKLSTDEMHEISALKA
                     ERDRVINSLQELDFDFKLGKIPAEDYPEQRAELLKKGSDILRKLDELEPILSSARDAA
                     SRIEKAAAAKRADSSSTASDMSDEDIEAMISARRKQHKSKSAGFCPSCGKPVLTADNF
                     CPSCGKSLK"
     gene            1924..>2947
                     /locus_tag="PKI33_08095"
     CDS             1924..>2947
                     /locus_tag="PKI33_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNM37008.1"
                     /translation="MKLRHLFLLAIGTLLLSACNMTLAADVTPPPGYVSPTPPPTLGP
                     LYPDKAPNVENGKAIFTEKCAACHGEKGLGDGEQSKDLPVSVIPIGLPEFANEATLSS
                     WYAVVTQGRIERFMPPFNSLTDQERWDVVAYALTLHTTNEQLELGKKLFEENCADCGD
                     AFTNLQMMSALSEDDIVKFVREGNGNFPAFGSNFSDEEAYAVAAYIHTLTFAVPSAPV
                     AAAATETPAAAESTPSTDSTPADGTPQAEGTPAATEAAVASMVKGLIINNSSADLSAD
                     QQITLRGFDHGSDPSAGPTEVLNVQGTVNPDGSYSFDVELLEGQIYLAELDVDGITYQ
                     SEFAVVP"
BASE COUNT          738 a          778 c          715 g          716 t
ORIGIN      
        1 ccggtcagca tgccaccttg cgtgatgatt tgtatatcct actcgtggac tggcagccag
       61 tttcgagcga gggtgccacc ttcaaaattt acgtcaaccc gttggtgaac tggttgtgga
      121 ttggcagcct gaccttctta ttcggtctca tctttgccgc ctggcccgac cgtgacccgg
      181 ctgatgagcc tctacgccgt aaggtgagtg cgggcaatcg ccaaacgagt gcagccgact
      241 aaaagagtca tcggtcgaag gtcaactttc gatcgatctc ggagaacttc aatgaagaaa
      301 ttcctcttta ttcttaccct tatgctggtt gcctctggtc tcttcacggc ggtggcattt
      361 gcccaagaca aaacccctac tgatgatgaa gtgaatgcaa ttgccaagca attgtattgc
      421 ccggtctgtg aaaacacgcc gttggatgta tgcccaaccg aagcttgtca tcaatggcgc
      481 gagttgatcc gccagatgtt ggcagaaggc aagagtgagc aacagatcaa agattatttt
      541 gtagcaaact acggcgctcg tgtgctctct gagccgccac gggctgggtt caactggttg
      601 gtgtatatca ttccaccagt attgatcctt gtgggagccg tcttcctctt caatgcgttt
      661 cgagcgtgga ccaagcccaa gtccgctgag gcaggttcag gtcaagcaga agaggcgggc
      721 acgtcatcca gtgacgatga atacattaag cgtcttgaag aagaattgaa gaacagaaaa
      781 taacagggac caatcggtct tttacggaga atgcaatgac agaaacaaca gcgccagcca
      841 gaaagagcgt ccccttgtgg gtgcaagtca ccatttgggt tttccttgcg ggtttgctcg
      901 cattggtggg tttcggtttg aaccgcgcca gccaccccat ggcagaagtg ggcgataccg
      961 tgcctgattt caagttggtt tattacgaag gctacgaaca caataatacg cctgagatga
     1021 agctttctga tttgcgcggc aaggttgtgc tcatcaacat ttgggcttca tggtgcaagc
     1081 cctgcgaaca ggaagctcct gaacttgaac aagcttggca gttatacaaa gaccaaggtg
     1141 atgtcgtttt catcggcgtg gattatgtgg atacgcccga aggtgcgttt ggctatttga
     1201 aaaagttcgg cattacattc cccaatgcac cagaccttca atcaaacatc tccagcattt
     1261 tgaatcgtca gatgggcgta cctgaaacct atttgatcga ccgcgacggt gtcttgcgcc
     1321 agatcaagat cggtcccttc gcatcggttt ctgaaattca agcttttatt gcaagtgcag
     1381 agtagggggt tctcatggaa ctaggttcaa tctttctcat cctcgcggtt ctcgtcgcag
     1441 taggaatgta tctatatgca cctttcatgt atcgttcgcg caaactttca acggacgaaa
     1501 tgcatgagat ctcagccttg aaagccgaac gcgaccgcgt catcaactcg ttgcaagaat
     1561 tggattttga tttcaagctc ggcaaaattc ctgctgaaga ttaccccgaa caacgtgctg
     1621 agttattaaa gaaaggctct gatatcttac gcaaactcga tgaactcgag ccgatccttt
     1681 cttctgctcg cgatgccgcc tcccgcattg aaaaagcggc tgccgccaaa cgcgcagatt
     1741 cttcctccac cgcttctgat atgagcgatg aagatataga agcaatgatc tctgcccgcc
     1801 gcaaacaaca caaaagcaaa tctgccgggt tctgcccttc gtgcggaaaa cccgtgctta
     1861 ctgccgataa cttctgccca tcttgcggaa agtctttgaa ataatccgca aaccgaggaa
     1921 ttcatgaaat tacgtcacct ctttctttta gcaataggta ccctcttgct ctctgcctgt
     1981 aatatgactc tcgcggcaga tgttactccg ccgcctgggt atgtatcccc cacgcctccg
     2041 cccacattgg gtccgctata tccagataaa gctcccaatg tcgaaaatgg caaagccatc
     2101 tttactgaga aatgcgcagc gtgccacgga gaaaaaggct tgggcgatgg cgaacagagc
     2161 aaagacctgc ctgtctccgt cattccaatc ggtttgcctg aatttgcaaa tgaagccaca
     2221 ctttcaagtt ggtatgccgt agtaacccag ggacgcattg aacgcttcat gccgcccttc
     2281 aacagcctca ccgaccagga acgttgggat gtggtggcgt atgctctcac acttcacacc
     2341 acaaacgaac agctcgaact cggcaagaaa ctctttgaag aaaactgtgc cgattgtggt
     2401 gatgcattca ccaatttaca aatgatgtcg gcattatcag aagatgacat cgtcaagttc
     2461 gtgcgtgaag gcaatggaaa cttcccagcc tttggaagca atttcagcga cgaagaagcg
     2521 tacgccgttg cagcctacat ccatacgttg acgtttgcgg ttccttccgc gccggtagct
     2581 gctgcagcca ccgaaactcc cgccgccgct gaaagcactc catccaccga tagcacacct
     2641 gctgatggca caccccaagc tgaaggtaca ccagcagcga cagaagctgc agttgccagc
     2701 atggttaagg gtctcatcat caataattca agcgcagacc tttctgcaga ccagcagatc
     2761 accttgcgcg gcttcgacca cggcagtgac ccaagcgcag gtccgaccga agtactcaac
     2821 gtgcaaggca ccgtcaaccc cgatggttca tattccttcg acgtagaact gcttgaaggg
     2881 caaatctatc tcgcagagtt ggatgtagac ggcattacct atcaatctga attcgctgtt
     2941 gtgcccg
//
LOCUS       DAOREF010000345         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22836_length_2938_cov_3.489074, whole genome shotgun sequence.
ACCESSION   DAOREF010000345 DAOREF010000000
VERSION     DAOREF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22836_length_2938_cov_3.489074"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PKI33_08100"
     CDS             complement(<1..1388)
                     /locus_tag="PKI33_08100"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37009.1"
                     /translation="MTSTKITVIGAGSASFGENTLSAIMRSKKLSGSTLALVDRNTDS
                     LDIVRRLAERLNREWDSGFKIESYSHHRDALPDSRFVVCAIEVGARENLWQQDFEIPL
                     KYGVRQPYAENGGPGGFAHAARNIRPILDIAHDMEQACPDAWFINFTNPMVRICDAVN
                     RYSKIKVVGLCHQIYIGYCYVGLVLGDDLGFDIPKGIRNMNAEVDQHDLREQVIGQAV
                     KRLDIRAAGTNHFSWILSMVDRETGEDLYPLFRERYARFNPDFEPLTRRVNDAFGLMP
                     VPGDSHLCEYLPWLSNPETKPWEKYNIQLYNWDERAALRELELERLNKMANNELPIDS
                     LLESDSEGALEMIENITCAGTHYHLAANLPNVGQIPNLPLNTIVETPVIVNGAGIQPV
                     HTGELPLAVAELCRRELFVSQLGVDAAVEGSREKALQCLLLDPVITDIEQAKLILDDY
                     LTTYKEYLPQFW"
     gene            complement(1411..2532)
                     /locus_tag="PKI33_08105"
     CDS             complement(1411..2532)
                     /locus_tag="PKI33_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNM37010.1"
                     /translation="MTNTNFPVQYPAHTPEPKKKISSRKIRDFFQSLFSHALINFIGL
                     FFLVPFLWMLITAFKSNQDVFHTPPRWLPYDNVLVDINGEQYPLYDVQTPDGVKQLAA
                     LKIVEGVGTFVDPASPTETFEYEMQQGNTKIAEPVMHVSFRWQNFPDAMNRASRPSID
                     ASFWVYFKNSLIIAFFSIVGTLLSNTPVAYAFARLKFPGRDFLFVIVLATMMLPYQVT
                     MIPLYLLFNNFLGWGDSFLPLIIPTFFANAYDVFLLRQFFRTIPEEMCDAARVDGASE
                     WQIFTQLVIPLSTPVLATVTVFTFLWAWNDFTGPLLFLTSPRNFTMALGLQDFQSQRT
                     LIWNQLMAASAIFTIPIIVAFFFAQKTFIQGIKLTGSKE"
     gene            complement(2537..>2938)
                     /locus_tag="PKI33_08110"
     CDS             complement(2537..>2938)
                     /locus_tag="PKI33_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM37011.1"
                     /translation="MWGMGNTIIIYLSGLQDVPLSLLEAAELDGASWWQRLWNITVPM
                     VSPITLFNLITGTIFMFQYFAQAYVFRGFQGLGYPLNSTLFYSVLLYQNAFQWLKLGY
                     ASAMAWVLFIIILVCTLLMMKISDKFTYYAG"
BASE COUNT          889 a          730 c          635 g          684 t
ORIGIN      
        1 ttccaaaatt gaggaaggta ttctttatag gtagtcaagt aatcatcgag gatcaatttt
       61 gcttgttcga tatcggtaat cactgggtca agcaaaagac attgcaaagc tttttcacgc
      121 gaaccttcta cagccgcatc cacaccgagt tgcgaaacga atagttcacg ccggcataac
      181 tccgccacag caagcggaag ttcgcccgta tgcacaggtt gaatccctgc accatttact
      241 atcacaggag tttcaacgat agtgttcaat ggcaaattcg ggatttgccc cacattcgga
      301 aggttcgccg ccaaatgata atgagtccca gcacaagtga tgttttcgat catctccagc
      361 gcaccttcac tgtcactttc aagtaacgaa tcaatcggca attcattgtt tgccatcttg
      421 ttcaggcgtt ccaattccaa ttcacgcaaa gctgcgcgtt catcccagtt ataaagttgg
      481 atattgtatt tttcccacgg cttggtttcg gggttactca accaaggcag gtattcacac
      541 aaatgcgaat caccaggaac aggcatcaac ccgaaagcat cgttcactcg acgagtcaac
      601 ggctcaaagt ctgggttgaa gcgggcatag cgttcacgaa aaagcggata caagtcctcc
      661 cccgtttcgc ggtcaaccat cgacagaatc cacgaaaaat gattagttcc tgccgcgcgg
      721 atgtccaacc gcttcacagc ctgaccaata acctgttccc gcaaatcatg ctgatccacc
      781 tctgcattca tgttgcgaat ccctttcgga atatcaaagc caaggtcatc acccaacacc
      841 aaccccacat aacaatagcc aatatagatc tgatgacaca agcccaccac ttttatcttg
      901 ctatatcggt tgacggcatc acagatccgc accatgggat tggtgaaatt gataaaccat
      961 gcatctgggc aagcttgttc catatcatga gcaatatcca aaataggacg gatattccgc
     1021 gcagcatgag caaaaccacc gggtccacca ttctcagcat atggttggcg cacaccatac
     1081 ttgagcggaa tttcaaaatc ttgttgccat aaattttctc gcgcacccac ctcaatggca
     1141 caaaccacaa agcgactgtc aggcaacgca tcacgatgat gtgaatagga ttcgatcttg
     1201 aagccagaat cccattctct attaaggcgt tccgccaacc tgcggacaat atctaggcta
     1261 tctgtatttc ggtccacaag agccaatgta gacccactta attttttgga acgcataata
     1321 gcagagagag tattctctcc gaatgaagcc gagcccgctc caattactgt aatctttgta
     1381 gatgtcataa tcaattccgt ggttaaaaag ttattccttc gaaccggtta acttgatgcc
     1441 ttgaataaag gtcttttgtg caaagaaaaa cgcaacgatg atcggaatcg taaagatggc
     1501 tgaagctgcc atcagctgat tccaaatcaa ggtgcgttga ctttgaaagt cctgcaaacc
     1561 gagcgccatc gtaaagttgc gcgggctggt caagaagagc agcggacctg tgaaatcgtt
     1621 ccaagcccac aaaaaggtga agacggtcac tgttgcaagc acaggcgtag aaagcggaat
     1681 aaccagttga gtgaaaatct gccattctga agctccatct acacgagcgg catcgcacat
     1741 ctcttcagga atggtgcgga agaattgtcg caacaggaag acgtcataag cattcgcaaa
     1801 aaacgtaggg atgataagcg gtaaaaaact atcgccccaa cctagaaaat tattgaacaa
     1861 gagatataaa ggaatcatgg tgacttgata cggaagcatc atggtcgcca agacgatcac
     1921 gaacaaaaaa tcacgccctg ggaatttgag acgcgcaaag gcatatgcaa ccggagtatt
     1981 cgatagcaaa gtccccacga tagaaaagaa agcaatgatc aaactatttt tgaaatacac
     2041 ccagaaactg gcgtcaatgc tggggcgaga agcgcgattc atcgcatctg gaaagttctg
     2101 ccaccgaaag ctgacatgca taaccggctc ggcgatcttg gtattccctt gctgcatctc
     2161 atactcaaac gtttcggttg gagaagccgg gtccacaaaa gtacccaccc cttccacgat
     2221 cttgagtgct gccaactgct tcacaccatc cggcgtctgc acatcgtaaa gcggatattg
     2281 ctcgccgttt atatcgacca gaacattatc gtagggtagc cagcgaggcg gagtatgaaa
     2341 aacatcctga ttcgacttaa acgccgtaat caacatccat aaaaatggaa cgagaaaaaa
     2401 caaaccgatg aagttgatca acgcatggct aaacagtgac tggaaaaagt cgcgaatctt
     2461 tcggctgctt attttctttt tcggctcggg cgtatgggca gggtattgaa cgggaaaatt
     2521 tgtatttgtc attggcttac cctgcgtagt aagtgaactt atcgctgatc ttcatcatca
     2581 gcaacgtaca aacaagaata ataatgaaca aaacccacgc catggcagag gcatacccga
     2641 gcttaagcca ttgaaaggca ttctgataca aaagcacact atagaagagc gttgagttca
     2701 acgggtaccc aagcccttga aaccctcgaa acacataggc ttgtgcaaaa tattggaaca
     2761 tgaagatcgt gcccgtgatt aggttgaaaa gcgtaattgg ggaaaccatt ggaacggtaa
     2821 tattccaaag tctctgccac caactggctc cgtccaactc ggcagcttcc aacaggctta
     2881 gcggcacgtc ttgcaagcct gaaagataaa tgatgatcgt attccccatc ccccacat
//
LOCUS       DAOREF010000346         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22941_length_2930_cov_3.421217, whole genome shotgun sequence.
ACCESSION   DAOREF010000346 DAOREF010000000
VERSION     DAOREF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22941_length_2930_cov_3.421217"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..2870)
                     /locus_tag="PKI33_08115"
     CDS             complement(165..2870)
                     /locus_tag="PKI33_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNM37012.1"
                     /translation="MIQAKDELTFGGWLRKRRRELDLTQQVLADRAGCTRITLRRIEA
                     GTLKPSGHLAETLLKLLGIPSQERKAWVQFARGIADHPTFAQFLLRTNLPSLLTSFVG
                     RKKEISEVKDALFHHRLVTLTGSGGIGKSRLSLQVAADLLTDFLDGVWFIELASLSDP
                     NLLPQTIQNILGLIEQKNTSPLQTLQEYLKEKKILLVLDNCEHLVEACATVAHTLLSH
                     TTSLKILASSREALGVQGEFAWRVPSLSVPDTDKGVEADQLSRYEAVKLFIERARLID
                     PQFVLDETNASFVARICSRLDGIPLAIELAAARLKTLTVEQIHARLDDRFSLLTNGAR
                     TLPSRHQTLRAAIEWSYTSLSENEKTLFRRLAVFTGGWSLEAAETICSGNGIKQEDVL
                     DLITSLLQKSLIHTSEFRPGVRYEQLETIRQYGQEKFLASDEVEILRNRHLQYFVELA
                     KRAEPELRSSAQVQWFTLLEQEISNLRSAMQWAKETDKTAFLQLTSSLWLFFRSLEHK
                     DEGIDCLLKAVSVNKDTPTPLLSTAMARLASLSIYLSIVQDQTEDYALTALDLSRQVR
                     DKPAEALALISLAGLELERMTGKFGPEYTELALGIARDLKDSWLISTALIQKGRFTQI
                     KNPIESVAIFEEALKEAQASGDKRLLYSNLFWMFINILATGQLARAKEIAHQCVLVAT
                     EIGDKDGIMYSHNALAGIGLFEEDYESSKEHAETIIRLSKAYQHNSGLLNGLGTAGLV
                     NLATKNISQVLELSTEMDRLILSKSGRLKSDTGYSVFLRIWAYILERDLKNARSNARE
                     LIPLYTQNDNILLSIEYFRVFAALAYMDGCHEINITLTSFAKKLHERVALAYFDYPFM
                     SRLRAEQLAQSREALGADAFNQAWEKGQQLDLEEAKKYTLEIANR"
BASE COUNT          744 a          687 c          695 g          804 t
ORIGIN      
        1 ttcctatatc acattagtca cttgttctgg gtacaacccg ctcaatgaaa gttatttctt
       61 tagaagaata gttcgagccg tgctggtcag cacggttagt gagtaacaaa acgagacctc
      121 cgaagtctta aagacttcgg aggtcttttt tatgagcaag ggcattatcg gttggcgatt
      181 tccaatgtgt atttttttgc ctcttccaag tccaactgct gtcctttttc ccaagcttga
      241 ttaaaagcat cggctcccaa ggcttcgcgt gattgggcta gttgttctgc ccgcagcctg
      301 ctcataaatg gataatcgaa gtaggctaaa gcaactcgtt cgtgcaactt cttggcaaag
      361 ctggtcaggg ttatatttat ttcatggcag ccatccatat atgccagcgc cgcaaaaacc
      421 cggaagtatt caatggataa aaggatgttg tcattctggg tatacagggg gatgagttct
      481 ctggcattac ttctggcgtt tttcagatca cgttcaagga tataagccca gatgcgaaga
      541 aacacagagt aaccggtatc agacttaagg cgaccactct ttgaaaggat caacctatcc
      601 atttcagtag atagttcgag tacttgtgaa atattttttg tcgccaaatt taccagcccg
      661 gcggttccaa gcccatttag tagaccagaa ttgtgctgat aagcttttga taatcgaatg
      721 atggtctcgg cgtgttcttt ggaggattca taatcctctt caaataaacc aatacccgcc
      781 aaggcattat gagaatacat aatgccgtcc ttatcgccga tctctgttgc aaccagtaca
      841 cattgatggg caatctcttt tgccctggca agctgccccg ttgccagaat attgataaac
      901 atccaaaata aattggagta cagcaggcgc ttatcgccag atgcctgagc ttctttgagt
      961 gcctcttcaa aaatagctac gctttcaatc ggattcttta tttgggtaaa tctccccttc
     1021 tgaatcaaag cagtggagat caaccaagag tctttgaggt cacgtgcaat tcccaatgcc
     1081 agctcggtat actccggacc gaactttccg gtcatgcgtt ccaattccaa gcctgccaat
     1141 gaaattaacg ccaaggcttc agcgggttta tctcttacct gcctgctcag gtcaagtgct
     1201 gtaagggcat agtcttccgt ttgatcctgc acaatagaaa ggtagatgga aagagaagct
     1261 agcctcgcca tggcagtcga aagtaaagga gttggtgtat ctttgtttac cgacacagct
     1321 ttcaacaaac agtcaatgcc ttcatcttta tgttccaatg acctaaaaaa gagccaaagg
     1381 gaactcgtca actgaaggaa ggcggtttta tccgtttctt ttgcccattg catggcagag
     1441 cgcaggttgg atatttcctg ttcgagaagg gtaaaccatt gcacctgcgc ggaactacgc
     1501 aattctggtt ctgcgcgttt tgctaactct acaaaatact gcaagtgccg attacgaagg
     1561 atctccactt catcactggc taagaatttt tcctgcccat attggcggat ggtctctaat
     1621 tgttcatagc gaactcctgg gcgaaattca ctggtatgaa tgagagactt ttgtaatagg
     1681 ctggtgatca ggtcgagcac atcttcctgt ttaattccat tccctgagca aatggtctcg
     1741 gcggcttcca aactccagcc accggtaaac actgccaacc ggcgaaataa ggttttttca
     1801 ttctccgata gggatgtgta gctccattca atagctgccc gcagggtttg gtgacgggaa
     1861 gggagagtcc gcgcgccgtt agtgagcaaa ctaaagcgat catcaagccg agcatggatc
     1921 tgttccacgg ttagggtctt caatctggca gcagccaact caatcgcaag aggaatgccg
     1981 tcgaggcggg aacagattcg ggcgacgaaa gaggcgttgg tctcatccaa aacgaattgt
     2041 ggatcgatca agcgcgcccg ttcgatgaat aacttcaccg cttcatatcg tgacagttgg
     2101 tctgcctcta ctcccttgtc tgtatctggc actgacagtg acggaacgcg ccaggcaaat
     2161 tcaccttgta cccccaacgc ctcgcggctg gatgccagaa tcttaaggga ggtggtatgg
     2221 ctcaacaagg tatgggctac tgttgcacag gcttcaacca ggtgttcgca gttatccaac
     2281 actaataaaa ttttcttttc cttcaagtat tcttgaagtg tttgtagggg agatgtgttt
     2341 ttttgttcga tcaaccccaa tatgttctga atggtttgcg ggagcaggtt ggggtctgag
     2401 agagatgcca attcgataaa ccaaacacca tcaaggaagt ctgtgagcag gtctgctgcg
     2461 acctgcagag ataggcgcga ttttccaatt ccaccagagc ctgtgagggt gacaaggcga
     2521 tgatgaaaca gtgcatcttt gacctcagat atttcttttt tgcgcccgac gaaactggtc
     2581 aacagggagg ggagattcgt tcttaacaga aattgtgcaa aagttgggtg atctgctata
     2641 ccccgtgcaa attgaaccca tgccttgcgc tcctggcttg gaatgccaag cagcttaagc
     2701 aaggtttcag ccagatgccc cgatggtttc aatgttccgg cttcgatcct gcgtaatgtg
     2761 atgcgggtgc acccggctcg gtctgcaagt acttgttggg tcagatccag ttcacggcgg
     2821 cgttttcgca gccacccccc aaaggtcaat tcatctttgg cttgtatcac aaatggtatc
     2881 ccttttggca ctcattttag aacgttcgta gggcaaatta tacaagactc
//
LOCUS       DAOREF010000347         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22947_length_2929_cov_4.219903, whole genome shotgun sequence.
ACCESSION   DAOREF010000347 DAOREF010000000
VERSION     DAOREF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22947_length_2929_cov_4.219903"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKI33_08120"
     CDS             complement(<1..444)
                     /locus_tag="PKI33_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37013.1"
                     /translation="MLSVTEARERILSHFKINSTETVSLIDAANRILGIDILADGDYP
                     PFDNSAVDGFALRAEDTSASPVTLNVVADIPAGSTPKVTLQAGQAARIMTGAQMPLGA
                     NCVIMVEDTDFNAKSAGTTAPKTVSFSKQMKSGENLRLRGADIRTG"
     gene            complement(515..2728)
                     /locus_tag="PKI33_08125"
     CDS             complement(515..2728)
                     /locus_tag="PKI33_08125"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNM37014.1"
                     /translation="MKSAVVEIPIEKLMEQLPESYTVADRELVMRAYSFAEEAHREQK
                     RNSGEPYINHCIAVASILTELKVPADVIAAGLLHDTVEDTPVTLADIRREFGDVVRLL
                     VDGVTKLSHLPRVSRGGEKAENAESEEEPPVQPALLGRKEDMVPETLRKTFLAMGEDV
                     RVVLIKLADRLHNMRTLGHMPEHKRRRIAAETLDIFAPLANRLGIWQLKWELEDLSFR
                     YVQPEKYKEIVESLAERRPDRERQLEEIKGNLIKLLEKNNIKAEVSGRPKHIYSIFKK
                     MQKKDKSFGMLYDVRAVRLIVPDVPSCYAALGVIHTTWRPVPGEFDDYIAAPKDNHYQ
                     SLHTAVMYDDKRPLEIQIRTQEMHMNAEYGIAAHWRYKEGGKHDKQFEERIKDLRKMM
                     EWGGEVNDATEFVESMRSDVFRDRVYIFTPRGDAIDLPAGSTPIDFAYTVHTDIGHRC
                     RGARVNGKLVPLDTELKTGDQVEILTAKRGGPSRDWLNNSLGLVKTARAKSKIRLWFK
                     KLEDEQNLATGRTTLERELQRLGIPEVNFEKLARDLGFKIPDEMFIGIGTGDLSMSKV
                     IKQFAEDEEIKDILEANTPAAPQKPTDAVEVLGLKGMLASMAKCCNPMPGDQIVGYIT
                     RGRGATIHRLDCPNILQLQDRERLLKVGWGHVEQTFPVPVKIKAYDRQGLMSDISNLL
                     SDENININNVSVNVNRSIADLRLIIEVKDLTQLSRILTRIENLPNVMEAVRNNPG"
BASE COUNT          664 a          728 c          730 g          807 t
ORIGIN      
        1 tccagtgcgg atatctgcgc ctcgcaggcg aaggttctct cctgatttca tctgtttgga
       61 aaaggagact gtcttggggg cggttgtccc tgcgctttta gcgttgaaat ctgtgtcttc
      121 taccataatg acacagtttg caccgagtgg catttgggct ccggtcatga tccgcgcggc
      181 ttgacccgct tgcagagtca ctttcggtgt ggatccagct ggaatgtcgg caaccacatt
      241 caaggtcacg ggagaggcgg aggtgtcttc cgcgcgcaga gcaaatccat ccactgccga
      301 attgtcgaac ggcggatagt ctccatctgc aagaatgtct attcccaaga tgcgatttgc
      361 cgcatctata agagaaacgg tttctgttga gttgatcttg aagtgggaaa ggatgcgctc
      421 gcgcgcttca gtaacggaaa gcatatcgaa gcgccgcgat tataccccca tctctttttc
      481 acaccttatt tcctgatgag aaaagcgaga atggttagcc tgggttattg cgtacggctt
      541 ccatgacatt cggtaggttt tcaatgcgcg tcagaatacg actcagttgt gtgaggtctt
      601 tgacttcgat gatgagacgt aaatcggcaa tgctgcggtt gacattgacg gagacgttgt
      661 tgatgttgat gttttcatcg gaaagcaaat tggaaatatc gctcatcaga ccctgacggt
      721 catatgcctt gatcttgact ggtacaggga aggtttgctc aacatgcccc cagccgactt
      781 tgagcaagcg ttcgcggtct tgtaattgaa gaatattcgg gcaatcaagg cggtgaatgg
      841 ttgcgccgcg tccgcgagtg atgtagccca cgatctggtc accgggcatg gggttacaac
      901 atttcgccat agacgccagc atgcctttca agccgagaac ttcaacggca tcagttggtt
      961 tttgtggggc ggcaggcgtg ttggcttcaa gaatatcttt gatttcttca tcttcagcaa
     1021 actgtttgat gaccttgctc atcgaaaggt cacctgtgcc aatcccaata aacatttcat
     1081 ctggaatttt gaagccaagg tcgcgagcta gcttttcaaa attgacttct ggaatcccaa
     1141 gcctttgtaa ctcgcgttca agcgtggtac gacccgtggc aagattttgc tcatcttcca
     1201 gcttcttaaa ccacaagcgg atcttggatt tagcgcgggc ggtttttacc aaacccaaac
     1261 tgttgttcaa ccaatcgcgg ctgggacctc cgcgtttggc ggtgagaatt tctacctggt
     1321 cgccggtttt taactctgta tccaatggca caagtttgcc attcacgcga gcgccacggc
     1381 agcgatgacc aatatcggta tggactgtgt aggcaaaatc gatcggggta gaaccagccg
     1441 ggaggtcaat tgcatcacca cgcggagtaa agatgtacac gcgatcacgg aacacatcac
     1501 tgcgcatcga ttcgacgaat tctgtcgcat cgtttacttc tccgccccat tccatcatct
     1561 tacgcaagtc tttgatgcgt tcttcgaatt gcttatcgtg cttgccgcct tctttatatc
     1621 gccagtgagc cgcgataccg tactcggcgt tcatgtgcat ttcttgggtt cggatctgaa
     1681 tctcaagcgg gcgcttgtcg tcatacatca cagccgtgtg tagagattga tagtgattgt
     1741 cttttggtgc ggcaatgtaa tcatcaaact caccgggcac ggggcgccaa gtggtgtgga
     1801 taacccctaa agcagcataa caagaaggga catctggcac gataaggcgc acagcccgta
     1861 catcgtataa catgccgaaa gatttatctt tcttctgcat cttcttgaaa atggaataga
     1921 tgtgcttggg gcgcccgctc acttcggctt tgatgttgtt cttttccaat agcttgatta
     1981 ggttgccctt gatctcttcc aattggcgtt cgcggtctgg gcggcgctca gcgagtgact
     2041 cgacgatctc tttatatttt tcaggctgaa cataacgaaa acttaagtct tccaattccc
     2101 actttaattg ccaaataccc agtcgattcg cgagcggggc aaaaatgtcc aaggtctctg
     2161 cggcaatgcg gcgacgttta tgttctggca tgtgacccag cgtacgcatg ttatgcaaac
     2221 ggtctgccag tttaatcagc accacgcgca catcttcgcc catggcaagg aaggtcttgc
     2281 gcagggtttc gggcaccata tcttctttgc gccctaacaa ggcgggttga acgggtggtt
     2341 cttcttcact ctcggcgttt tctgcttttt caccaccgcg cgaaacacgc ggcaaatgag
     2401 ataacttggt cacgccatca acaagtaaac gcaccacatc cccaaactcg cggcgaatat
     2461 ctgcgagagt tacaggtgta tcttctactg tgtcatgtag caaaccagca gcaataacat
     2521 cggcaggcac tttcaactct gtcagaatac tggctacagc aatacagtga ttgatatagg
     2581 gttcccccga attgcgtttc tgttcgcggt gcgcttcttc cgcaaaggaa tatgcacgca
     2641 ttacgagttc ccggtctgca acggtataag actcggggag ttgttccatt aatttttcaa
     2701 tagggatttc tacgactgcc gatttcatgc gcttaatttt actacgcccg cctctctccc
     2761 ggactttgat atgcctttgt ttcctgatgg cttgctctcg ctgaggcttg atataaaaac
     2821 cgctggcttg aaaaagccag cggttcactt tattagatgg tcttcaacaa ggcttggtgg
     2881 ctatttcgca aacggtctgc gatcacttcg gcaatgcgtt tcatgactg
//
LOCUS       DAOREF010000348         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22949_length_2929_cov_3.900487, whole genome shotgun sequence.
ACCESSION   DAOREF010000348 DAOREF010000000
VERSION     DAOREF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22949_length_2929_cov_3.900487"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..2342)
                     /gene="recJ"
                     /locus_tag="PKI33_08130"
     CDS             complement(57..2342)
                     /gene="recJ"
                     /locus_tag="PKI33_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNM37015.1"
                     /translation="MTRWLDPQPVDIPASFQNLGLPPLIAQTLLRRGISSPVEAEAFL
                     YPEKNPNSQFPNIEKAVELLEMAIRNREKICVWGDFDVDGQTSTALLVQTLRDLNADV
                     MYYVPVRGKESHGIHIESLKPIIDNGAKLLLTCDTGITAHDAIDYANSRGLTVIVTDH
                     HDLGETLPNAQAIINPKLLPEDHPLRNLAGVGVAYKLAEALLDTRTSNLDSQISNIEL
                     LDLVALGLIADVALLQNETRSLAKQGIEQLRNTSRLGLRVMAELAGAAFETLTEETIG
                     FTFAPRLNALGRLGDANPAIDLLITDDSARARVLATQIEGLNAQRRMLTKQVNDAAEA
                     QLKENPELLNHPIIILSHPNWPGGVVGIVANKLVERYHKPAILFNESEDGILRGSARS
                     IEGLHITEAITTQKDLLIGFGGHPMAAGMALHKENLSAFRKGLGKAIEKQLGDIVFEE
                     PTLQIDAWLGLTDLSLDLADSLELLAPFGAGNPQLTLATRNVMLKSAISLGKTKEHLR
                     LNVEDENGEVASFLWWGGAGEELPPKDSKFDIAYTLRATSFRGQRQVTLQFKEFRVTE
                     ERPIEVKEAKLDIRDMRLQHTLVNAQPSALIWAEGPDRAIGVNRLELTQAKELTIYTT
                     PPSSIELRKALDIVQPEVVYFFGISPAEQSPEEFLTYLAGLCKFVLNQRHGKTTISEL
                     AAASAARESAIQLGLEWLAAGGQLTVTLEENEVQLSKETQEKNPYLQMELFIALRGVL
                     SETSAYRKYFATVPDLKTVFR"
     gene            complement(2384..2584)
                     /locus_tag="PKI33_08135"
     CDS             complement(2384..2584)
                     /locus_tag="PKI33_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNM37016.1"
                     /translation="MNNNLKVLRAEKNWSQADLAEKLNVSRQTVNALETGKYDPSLPL
                     AFKIADLFGKKIEEIFFPEKEN"
     gene            complement(2581..>2929)
                     /locus_tag="PKI33_08140"
     CDS             complement(2581..>2929)
                     /locus_tag="PKI33_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37017.1"
                     /translation="FIAIGAYVVILIGSISILKYLELPKSAQFIVAILPIIPILFVII
                     AIMRALRDSDELQQKIQSYAIMFSAVATGLITFSYGLLENVGFPPFPTIWVLPMMFMF
                     WGFSLSYFWKKYQ"
BASE COUNT          730 a          626 c          779 g          794 t
ORIGIN      
        1 tgcgatagta gttgccggta caaataaata aaaccctatt tgtcattttg ctttttttat
       61 ctaaaaacag tttttaggtc tggaactgtc gcgaaatatt ttcggtatgc agaggtttca
      121 cttaagacgc cacgtagcgc aatgaaaagt tccatctgta gatacggatt tttttcttgt
      181 gtctcttttg aaagctggac ttcattttct tcaagagtca cggtcaattg accgccggct
      241 gccagccatt ccaaaccgag ttgaatggca gactctcgtg cggcggatgc ggcggcgagt
      301 tcagaaatgg tggttttccc atggcgttga ttaaggacga atttgcataa ccctgctaga
      361 tacgtgagga attcttcagg gctttgctcg gcaggggaaa taccaaagaa gtaaacaacc
      421 tctggttgga caatatccaa ggcttttcgt agttcaatag aagaaggcgg cgtcgtatag
      481 atggtcaatt ctttagcttg agtcaattca agacggttga ctccgattgc gcgatcgggt
      541 ccttctgccc aaatgagggc ggatggttgt gcgttaacca aagtatgttg caaacgcata
      601 tctcgaatat cgagctttgc ttctttgact tcgatagggc gttcttcagt aactcgaaat
      661 tctttaaatt gcagcgtaac ttgacgttgc ccacggaagg atgtggcacg taaagtgtag
      721 gcaatatcga atttactgtc tttgggtgga agttcttctc cggcgccgcc ccaccataag
      781 aaactggcaa cctcaccgtt ttcatcttct acgtttaggc gtagatgttc tttggtcttc
      841 ccaagcgaaa tggcagactt gagcatgaca ttgcgcgtgg cgagggtcag ctgtgggttg
      901 ccggcgccga aaggagcgag tagctccaag ctatcggcga ggtcaaggct caagtcggtg
      961 agacccaacc aggcgtcgat ctgaagcgtg ggttcttcaa agacaatatc cccaagttgt
     1021 ttttcaatcg ctttgccaag tcccttgcgg aaggcagaca gattttcttt gtgtaatgcc
     1081 atgcctgctg ccatgggatg cccaccgaag ccgataagca agtctttctg tgtggtgatg
     1141 gcttcggtaa tgtgcaaccc ttcaatagaa cgtgccgagc cgcgcaagat gccatcttca
     1201 gactcgttga aaagaatggc tggtttgtga taccgctcaa cgagtttatt ggcaacgatg
     1261 cccaccacac caccgggcca gttaggatgc gaaagaatga tgatgggatg attaagcagt
     1321 tcggggtttt cttttagttg tgcctccgcc gcatcgttga cttgcttggt gagcattcgg
     1381 cgttgagcgt ttagcccttc aatttgagta gcaagcactc gcgcacgtgc agagtcatct
     1441 gtaattaata agtcaatggc ggggttggca tcacccaaac gaccgagtgc gttgagacgt
     1501 ggagcaaagg taaagccaat cgtttcttcg gtgagtgttt caaatgcggc gccagccagt
     1561 tctgccatca cacgcaaacc aaggcgactt gtatttctta gttgctctat gccttgtttg
     1621 gcaagtgagc gggtttcgtt ttgaagcaag gcaacatcgg ctatcaagcc aagagctact
     1681 aaatcgagta attcgatatt cgatatttga gagtcgaggt tcgatgttcg agtatcgagc
     1741 aacgcttcag ctagtttgta cgcaacccca acacccgcca aatttcgcag tggatgatct
     1801 tctggcagaa gcttggggtt gatgatggct tgtgcatttg gcagggtttc acccaaatca
     1861 tgatggtctg taacgatgac tgttagtccg cgagagttgg cgtaatcaat ggcatcatga
     1921 gcagtgatgc cggtatcgca agtgagcagt agctttgcgc cgttatcaat aattggttta
     1981 agcgactcga tgtgaatgcc gtggctttct tttccgcgca cgggcacgta atacatcacg
     2041 tctgcattca gatctcgcaa cgtttgcaca agcagggctg tggaggtttg cccgtctacg
     2101 tcaaaatcac cccagacgca gattttctct ctattgcgga tagccatttc aaggagttcg
     2161 actgcttttt cgatattggg aaattgagag ttgggattct tttcgggata aagaaacgcc
     2221 tcggcttcga cgggggagct gattccgcga cggaggagag tctgcgcgat gaggggaggc
     2281 aagcccaagt tttggaaaga ggcgggaatg tctacaggtt gagggtcgag ccagcgagtc
     2341 ataaaaatag aaccatccaa aaaagatggt tctattgtag cagttagttt tctttttccg
     2401 gaaaaaatat ttcttcgatc tttttcccga atagatcggc gatcttgaat gcaagcggaa
     2461 ggctggggtc gtacttgccg gtttcaaggg cgttgacggt ttgccttgag acattcaatt
     2521 tctctgcaag gtcggcttga gaccagttct tttctgcgcg caagaccttg agattgttgt
     2581 tcattggtat ttcttccaga agtagctaag gctaaacccc cagaacatga acatcatggg
     2641 caatacccaa atggtaggga agggtggaaa gcctacgttt tcgagcaagc catagctgaa
     2701 tgtgatcaag ccggttgcca cagcagagaa cataatggca taggactgaa ttttttgctg
     2761 aagttcgtca ctatcgcgta gtgcgcgcat gatggcgatg atgacgaaga gaatagggat
     2821 gatgggcaaa atggcaacga tgaattgggc agatttgggc aattcgaggt attttagaat
     2881 gctgatactg ccgatcaaaa taaccacata ggcaccaatg gcgataaaa
//
LOCUS       DAOREF010000349         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22962_length_2928_cov_4.374173, whole genome shotgun sequence.
ACCESSION   DAOREF010000349 DAOREF010000000
VERSION     DAOREF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22962_length_2928_cov_4.374173"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1199)
                     /gene="add"
                     /locus_tag="PKI33_08145"
     CDS             complement(159..1199)
                     /gene="add"
                     /locus_tag="PKI33_08145"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNM37018.1"
                     /translation="MADDTTTPFNKYVALPKVELHRHLEGSLRLSTMLDIARKHGVTV
                     PVSMLNLSGLVQVQDQDPMTFSNFLEKFKTLRLFYRSPDVIHRVTREAVEDAARDNVR
                     YMELRFTPVALSRAEGFPLHDVMDWVITSAQEAAREYKIKVGLIASVNRHESPELAEQ
                     VSWLAVEHMKNGLFGLDLAGNEAEFKSHPFRDIFKEAKQSGLRITIHAGEWGPADNVR
                     DAIEHLGAERIGHGVRVLEDEAVAALAMERGAVFEVCVTSNFQSGVINEVEKHPLPRM
                     LEKGLKVTVNTDDPSVSRITLSNEFQSIHEKLNISMDVLKQSVITSAEASFLPDAEKA
                     DLVKKLKEELKR"
     gene            complement(1217..>2928)
                     /locus_tag="PKI33_08150"
     CDS             complement(1217..>2928)
                     /locus_tag="PKI33_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37019.1"
                     /translation="QGDPGSSRFYLSLEDDLMRIQGGAQVSGLMERLKVDDSLPLEAR
                     IVASVIEQSQHRIEGANFDVRKHLLEYDDVLNTQRQQIYLQRDKIFVKEDLSGDVAEM
                     LDNEVTKRVEVGLADEEGPWRLLAWLDQVQPAFDSKNGLFPSYGFKLLLDQINPSDVR
                     NSTLDLVSKSIEAEYAHALRAIETMIEKTGETLETQIAEREDSLDAFFQTLGDREDAP
                     KAQKLVEEINSLTRLAIRPSNDLLKKLADDPEEAKEDIQEMIASQLTIISATRVIGAI
                     QNRIGEQVQWQNPLPSDWDELSDILLTTSRDAFEKKRERLNAQVVRDIDVLLQREDVS
                     TESGRLRLLITLSQGTRTAFDQKTHKQVKQVFTRFAYAFYAAQLLEGREAEEIADDVM
                     NHLEAAEESLRETWGQSEFVRLSQNATKLADFGPAARIAFGEEKLNNAPSSLSADEAA
                     QLSSALGKYVLNEVHRQLLLGAFSELWVEYLTKVEALRVSIGLEAYAQRDPLVQYKRS
                     ASEMFQQLLEDVRGLVISRAFAARPRRVEITPIETTETAATPNETSVQIGGDSKKKKR
                     RRS"
BASE COUNT          702 a          805 c          685 g          736 t
ORIGIN      
        1 acactcacac cacaatcagg aagaacacaa aggagcgcct ttgatatcct tttttaatgt
       61 ggtggggtta acgagagaaa ttcagagcta aattatacta attcaagggc gtgcgtggaa
      121 gtgtcttttc caaatcacgc cctatttacc ggactgtctt aacgcttcaa ttcttccttg
      181 agttttttga ccaaatccgc tttttcagca tcgggcaaaa aggacgcttc tgccgacgtg
      241 atcacagact gtttcaaaac gtccatcgaa atgttcaatt tctcgtgaat ggactggaat
      301 tcgttcgaaa gagtgatgcg cgaaacactt gggtcatcgg tattgaccgt gaccttgagt
      361 cccttttcga gcatgcgcgg caacggatgt ttctccactt cattgatcac gcccgattgg
      421 aagttactag tcacgcagac ctcaaagacc gcgcctcgtt ccatcgccaa agctgccaca
      481 gcttcatctt ccaacacgcg cacgccgtga ccgatacgct ccgcgcctaa atgctcaatc
      541 gcatcgcgga cgttatccgc gggtccccac tccccggcat ggatggtgat acgcaaaccc
      601 gattgtttgg cttccttgaa aatatcacgg aagggatgag acttaaactc cgcttcattg
      661 ccagccaaat ccaagccaaa caaaccgttc ttcatatgtt ccaccgccaa ccacgaaacc
      721 tgttccgcaa gttccgggct ttcatggcgg ttcaccgaag cgatcaaccc aaccttgatc
      781 ttgtattctc tggcagcttc ctgcgcactg gtaatgaccc aatccattac atcatgcagc
      841 ggaaagccct cggcgcggct caaagccacg ggcgtaaaac gcaactccat gtagcggacg
      901 ttgtcacgag ccgcatcttc caccgcttca cgagtgacgc gatgaatcac atcgggtgag
      961 cgatagaaca aacgcaaggt cttaaatttt tcaaggaagt tcgagaaggt catcgggtct
     1021 tggtcttgca cttgcaccag cccgcttaag ttcaacatcg aaaccggaac cgtcaccccg
     1081 tgtttgcgcg caatatccaa catggtggag agacgcaaag acccttccaa atggcgatga
     1141 agttccacct ttggaagggc aacatattta ttaaaagggg ttgtagtatc gtctgccaaa
     1201 cgcttcctct tacgagttac gaacggcggc gcttcttctt tttgctatca ccgccgattt
     1261 gcaccgacgt ctcgttcggt gtcgcagctg tttctgtggt ttcaatcggt gtgatctcca
     1321 cccgtcgcgg tcgtgccgca aacgcacggc tgatcacaag tccgcgtaca tcttcgagca
     1381 attgctggaa catctctgat gcactgcgtt tgtattgtac cagcgggtca cgttgggcgt
     1441 aagcctcaag acctatcgaa actcttaagg cttcgacctt ggtgaggtac tccacccaca
     1501 gttcactgaa cgcacccaat aacaactggc gatggacttc attcaataca tacttgccca
     1561 atgcagagga tagctgcgcc gcctcgtccg cgctcaggct ggatggagcg ttattcagct
     1621 tttcctcgcc gaaagcgatt cgcgccgccg gaccaaagtc cgcgagcttg gtggcgttct
     1681 gactcaaacg cacgaactcg ctctgtcccc acgtctcgcg cagggattct tctgccgctt
     1741 cgaggtgatt catcacatca tcggcaatct cttcggcttc gcgaccttcc agcaattgag
     1801 cagcatagaa ggcatatgca aaacgggtaa agacctgctt aacctgttta tgggttttct
     1861 ggtcaaaggc agtgcgggtg ccttgtgaga gggtgatcaa caaacgcaat cgccccgact
     1921 cggtcgagac atcttcacgc tgtaacaaca cgtcaatatc acgcaccact tgagcgttca
     1981 agcgttctcg cttcttttca aaagcgtcgc gtgaagtagt caacaaaata tccgaaagtt
     2041 catcccaatc cgaagggagc gggttctgcc actgaacttg ttcaccgatg cggttttgaa
     2101 tagcaccaat cacgcgagtc gcactgataa tggtcaactg gctggcaatc atttcctgaa
     2161 tatcttcttt tgcctcttca gggtcatctg ccaatttctt gagcaagtca ttgctcgggc
     2221 gaattgccaa gcgggtcagg ctgttgatct cttccaccaa cttctgggct ttgggcgcat
     2281 cttcacggtc acctaaggtt tggaagaagg catcgaggct gtcttctcgt tctgcaattt
     2341 gcgtctcgag agtctcgcct gtcttttcga tcatcgtctc aatggcacgc aaggcatgag
     2401 cgtattcagc ttcgatggat ttagagacca agtccaaggt cgaattgcgg acgtcggaag
     2461 gattgatttg gtcaagcaat agcttaaatc cataagaggg gaacaagccg ttctttgaat
     2521 cgaaagcagg ttgaacttgg tctaaccacg ccaataaacg ccacggacct tcttcgtctg
     2581 ccaagccaac ttctacgcgt ttggtcactt cgttatcgag catttctgcc acatcgccgc
     2641 tcaaatcttc tttgacgaag atcttgtcgc gctgtaagta gatttgctgg cgttgagtat
     2701 tcaagacatc atcgtattcc aagagatgtt tgcggacatc aaagttcgca ccctcaatac
     2761 gatgttggga ctgttcgatc acactcgcta caatacgggc ttccagcggg agagaatcgt
     2821 ccactttgag gcgttccatc aagccgctaa cctgtgcccc accttgaatg cgcatcaggt
     2881 catcttccag cgaaagataa aaccgcgaag atccggggtc accctgac
//
LOCUS       DAOREF010000350         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_22963_length_2928_cov_3.860425, whole genome shotgun sequence.
ACCESSION   DAOREF010000350 DAOREF010000000
VERSION     DAOREF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22963_length_2928_cov_3.860425"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKI33_08155"
     CDS             <1..372
                     /locus_tag="PKI33_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNM37020.1"
                     /translation="ARPVLVTVDGQRFEVMPESGTQAQTKNEAVSNIEVKPTMPSPLG
                     VANSSVSGSSSLHAPLPGTVVEVFVKAGDSVEAGQVILVIEAMKMKNSIRTTRSGTIA
                     NVLVNQGQSVAHKQTLVEFTS"
     gene            420..1553
                     /locus_tag="PKI33_08160"
     CDS             420..1553
                     /locus_tag="PKI33_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNM37021.1"
                     /translation="MDLNSLLPELLKGFSHFTLGNGVMIFAALVLIYLAVFKEIEPVL
                     LLPIGFGCLLANIPLAGMTAAEGMTKVIYDAGIKTELFPLLIFIGVGAMIDFSPLLAQ
                     PRMVLLGAAGQFGIFGTLMLAVALGFPLNQAASIGVIGAIDGPTSIFVATKLAPELLA
                     PIAVAAYSYMSLIPIIQPPLMKLFTTKKERLIQMEYTPTPISKTTLIFFPVVLTLVVG
                     LLVPEATPLISMLMLGNLLKVTGVVDRLSNAAQNEIINIATLFLGLAIGSTMNAAAFL
                     NLATGKIMLLGLFAFALDTVAGLLFGKFMAFITGGKINPLIGAAGISAFPMAGRLAAK
                     TANDEDNNNFILMHAMGANTAGQLGSVIAGGILLSVVSKILGI"
     gene            1580..1909
                     /locus_tag="PKI33_08165"
     CDS             1580..1909
                     /locus_tag="PKI33_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37022.1"
                     /translation="MKLKKEAQMKLEGSFIFASLAFLFAIGSIIYLKRMQNVTENDIK
                     HRIRTEYPPESRKQILEFYRRLKIKEMEGLFSKILDDAKGDVNKVGKLTNVAESIGWK
                     AFLENEW"
     gene            complement(1949..2452)
                     /locus_tag="PKI33_08170"
     CDS             complement(1949..2452)
                     /locus_tag="PKI33_08170"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HNM37023.1"
                     /translation="MTENEQLNPNEPIDDKYVLDKYNNKISYYWGASRSNKNSYKRYR
                     AWTVILGALVTLVSSISAAEFVQKPEWLRVAFAVATPVIAATLTVINGLGQNFHWGAT
                     WRDMVLNGTRLEKERDRFLATKPEDRDLVKELDRLNTIVLDETRNFFQRVLDSEVKPG
                     KDQNGNG"
     gene            2603..2890
                     /locus_tag="PKI33_08175"
     CDS             2603..2890
                     /locus_tag="PKI33_08175"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNM37024.1"
                     /translation="MTAFVILDIEVTDPVGYEDYKKLAAPSLEPYGGKYAARGGKVEN
                     LEGDWSPTRIVVIQFESVERAKAWINSPEYSEARALRHKYAVSKAIVVEGL"
BASE COUNT          783 a          652 c          671 g          822 t
ORIGIN      
        1 gcccgtccgg tcttggtgac ggtagatggg cagcggtttg aggtcatgcc tgagagtggc
       61 actcaagctc aaacaaagaa tgaagcggtt tctaatattg aagtcaaacc tacgatgccg
      121 agtccacttg gggtggcaaa ctcatcagtt agcgggagtt catctctcca tgctccgcta
      181 cccggcaccg tggtggaagt gttcgtgaaa gcaggcgact ctgtggaagc cgggcaagtg
      241 atattggtca ttgaagcaat gaagatgaaa aacagcatcc gcacaacacg cagcggcaca
      301 atagccaatg tgcttgtaaa ccaaggtcaa agtgtggctc acaaacaaac cctggttgaa
      361 ttcacatctt aattttacga atgacttaat aggcattgct gcattcgtaa aggactctta
      421 tggacttaaa ttctttgctt cctgaacttc taaaaggctt ctcgcatttc actcttggta
      481 acggtgtgat gatctttgcg gcattggttt taatttacct ggctgtcttc aaagaaattg
      541 aacctgtttt gttattgccg atcggttttg ggtgtttgct ggcaaatatt ccactcgctg
      601 gtatgaccgc tgcagaaggc atgaccaagg tgatttatga tgctggcatc aagactgaac
      661 tttttccact gctgattttt attggcgtcg gtgcaatgat cgacttctcg ccactgttgg
      721 cacaaccacg catggttttg ctcggtgcag caggacagtt cggcatattc ggcaccttga
      781 tgctggctgt agcattgggc tttccgctca atcaagctgc gtctataggt gtgatcggcg
      841 ccattgacgg accgacatct atttttgtag caaccaaact tgcgcctgaa ttgcttgctc
      901 ctatcgcggt ggcagcatat tcctatatga gtctgattcc catcattcaa ccacctttga
      961 tgaagctctt tactacaaaa aaagaacgtt tgattcagat ggaatacaca cctacaccca
     1021 tctcgaaaac aacgctgatc ttcttccctg tggtattgac actggttgtg ggtctgcttg
     1081 tgcctgaagc gacgccgctt atttcaatgc tgatgctcgg gaatttgcta aaagtcacag
     1141 gtgtcgttga tcgcttgagc aatgccgcac agaatgagat tatcaacatc gcgacattgt
     1201 ttcttggttt agccatcggt tcaacgatga atgcggctgc ttttctgaac ttggcgaccg
     1261 gcaaaatcat gttactcggt ctattcgcct tcgcgcttga tacggttgca gggttgttat
     1321 ttggaaaatt catggcattc atcacaggcg ggaagatcaa tccgctcatt ggggcggctg
     1381 gcatctctgc ctttccgatg gcagggcgcc ttgctgcaaa aaccgctaat gacgaggaca
     1441 acaacaactt tattctcatg catgccatgg gcgcgaatac agccggtcaa ctaggaagcg
     1501 tgatcgcggg tggaatattg ctttctgtag ttagcaaaat actaggcatt taaggaactc
     1561 aactggcttt tttgctacaa tgaaactgaa gaaagaggcg caaatgaagc tggaagggtc
     1621 attcatcttt gcaagtttag cttttctgtt tgcaattggt tccatcattt atctgaaacg
     1681 catgcaaaat gtgacagaaa atgacataaa acacagaata cgaacagagt atccgccaga
     1741 gagccgaaaa caaattttgg aattctatcg gcgtctaaaa attaaagaaa tggaagggct
     1801 tttttccaaa attcttgacg acgccaaagg ggatgtaaac aaggttggga aactaacaaa
     1861 tgtcgctgaa agcatagggt ggaaagcatt tcttgaaaac gagtggtgat taaaagaaac
     1921 agggcgaatt tcttcgccct gtttcttttt agccattccc gttctggtct tttcccggtt
     1981 ttacttcgct atctagcact cgttggaaaa aatttctggt ttcatcaagc acaattgtgt
     2041 ttaaccgatc caattctttt accaagtctc tatcttccgg cttggttgca aggaaacggt
     2101 ctcgttcttt ctcaagacga gtgccattta agaccatatc ccgccaggta gcaccccaat
     2161 ggaagttctg tcccaatcca ttgatcaccg tcaacgtggc agcaatgacc ggagtcgcca
     2221 cggcgaaagc gactctcagc cactccggct tttgcacaaa ttctgcggct gagattgacg
     2281 aaaccaaggt tacaagcgca ccaaggatca ccgtccaagc acggtagcgt ttatacgagt
     2341 ttttattact acggctggcg ccccaataat aggaaatctt gttgttgtac ttatctaaaa
     2401 catatttatc atcaataggt tcatttgggt tgagttgctc attttcagtc attgtcctac
     2461 ctccacaatc gggatgcgtt ttttcttacc actattatga cactttttta caaaatttca
     2521 caggaatttt tttaccctca caagagtcaa tgttagaatc ttgtgtcaca caacctatcg
     2581 ctaaatcact aaggagtcag atatgaccgc atttgtaatt ctagacattg aagtaacaga
     2641 ccctgttgga tacgaagatt ataaaaaact ggcagcgcct tctctcgaac cctacggcgg
     2701 gaaatatgca gcccgtggtg gaaaagttga aaaccttgaa ggtgattggt cacccacacg
     2761 tattgttgtg atccaatttg aaagcgtgga acgcgccaaa gcatggatca actcgccaga
     2821 atacagcgaa gcgcgtgctt tacggcataa atacgcagtt tcgaaagcca ttgtggtgga
     2881 agggctataa attgaagtct gtcgaaaatt ttattgccga attcaaag
//
LOCUS       DAOREF010000351         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23115_length_2915_cov_4.587063, whole genome shotgun sequence.
ACCESSION   DAOREF010000351 DAOREF010000000
VERSION     DAOREF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23115_length_2915_cov_4.587063"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..>2915
                     /locus_tag="PKI33_08180"
     CDS             190..>2915
                     /locus_tag="PKI33_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM37025.1"
                     /translation="MNFNSSNGRFSEKSIGRVLTGIAFLYQAIAVVVVVWALVVAAQW
                     IREPFLGSFYEHTLVFTDPAADSTGVWAFNKIVNSGDQLIAINGTDVKNSRDVREVLS
                     SFVPGENVTATVKFKNGETRDLSVELQRFPEDAVSIYLILPSIISFVFLVLSIWTFGL
                     RRNEPSGRAFALFASSFAIVTGTIFNLWTSHEFTTIWTAAVAIAGGANIALALSFPAS
                     PRLIINRPYLRWMGFVLSFVLTAITIPYLFNSQNPTEYIAKWQNLYYFAAVGILSLLG
                     SNLYYSLYSQSPVVKLQARTTLIGAVFAFLPLTVYLVFGNIFSIKFSPWLFLPVVIFP
                     AIMGYNIMRFRFLRTDDLARRGVMYVLLTAIVTMGYALVAAGAAFLFGNSLPQNYLAG
                     FFILVLAIVLEPLRIRLQNLTDVLFFRGSRVVAEQLEDFSHKLTTALDLNAISSLLRD
                     QIASTLAPDQIHIFTYDSLNDFFVASPGDGRRTTTDIRFTSASPLVSYFRSERLPLYL
                     DNGTDLPDVLKPEQSRIALLGARLFIAFPGKDKPNGWLALGPRLTNQAYSPSDLRFLE
                     NISDQASVAIERVQTVAHLERRIQEMNALTRVSQGVNVTLTFDDVLELIYAQTAQIVP
                     TSHFHITLYDKDSDYYYYGFCLENNERMHERENQPFSSNVGLSPDVIRKSRPIITQDY
                     IRECQARNHTPTIENIFAWIGVPLNAGAESIGALSVGSRDASTIYTRAQVDLLQAIAD
                     QTAGAIVKARLLKETQERAHQLTTLNEITRQLTSTLELNPLLQNILENAVGILNCEAG
                     SLLLVDDQTDELVFRVTVGPVAGNLIGKRLARGTGIVGRAVETRKPVIENEEQTSNRF
                     NGVDQQTGFVSRSLLAVPLQAKDRVIGVVEVINRCDGLPFVQNDQTLLTAFAG"
BASE COUNT          649 a          689 c          723 g          854 t
ORIGIN      
        1 gaaccgtaag atattaaacg gcgcgatgag cgccgttttg cttaggctac agagtagagg
       61 attttaggat gggagaaaca ggcaaaagtc cagaaagagg cgggttttcc tatcccacct
      121 tacgcctttg tcatgaaagt accatatgat ttgggcggga ttgtagagta aaatttcagt
      181 cattaagcta tgaatttcaa ctcatccaac ggtcgattct ctgaaaagtc catagggcgc
      241 gtcctgacgg ggattgcgtt cttgtatcag gcgatagctg tggttgtcgt tgtgtgggcg
      301 ctggtggtgg ctgcacagtg gatccgtgag ccttttttgg gctctttcta cgagcataca
      361 ctggttttta cagacccagc agccgactcg accggggttt gggcgttcaa taagattgta
      421 aatagcggcg atcagttgat tgccatcaat gggacggacg ttaagaacag ccgtgatgtg
      481 cgtgaagtac tttcaagttt tgtgcccggc gaaaatgtaa ctgcaactgt aaaatttaag
      541 aatggcgaga cgcgcgacct gagtgtggag ttgcagcgct tccctgaaga tgcggttagt
      601 atttatctca ttctcccgtc gattatcagt tttgtttttc ttgtccttag tatttggact
      661 tttggtttac gtcgcaatga accttctggt cgtgcgtttg ctttatttgc atcctctttt
      721 gcaattgtca ccggaacgat cttcaacttg tggaccagcc acgaatttac aaccatatgg
      781 acggcggctg ttgctattgc gggtggggca aatattgcgt tggctttatc gtttccggct
      841 tcaccaaggc ttattatcaa tcgtccttat ctccgttgga tgggttttgt tcttagtttt
      901 gttttgacgg cgattacgat cccatatctt ttcaattcac aaaaccccac cgagtacatt
      961 gcaaagtggc aaaatcttta ctactttgcg gctgttggta ttttgtcgct ccttggatcc
     1021 aatttatatt attcactgta ttcacaatcg cctgttgtta agctacaagc acgcactaca
     1081 ttgatcggcg ccgtctttgc ctttttgccg ttgactgtct atttggtctt cggcaatatt
     1141 ttctccatca agttttctcc gtggctgttt ctgccggttg tgattttccc cgcgatcatg
     1201 ggctacaaca tcatgcgctt ccgcttcttg cgtactgatg atcttgctcg gcggggtgtg
     1261 atgtatgttt tgttaactgc aatcgtcact atgggctatg ccctagtagc tgctggtgct
     1321 gcgttcttat ttggaaactc cctgcctcag aattatctgg cgggcttttt cattttggtg
     1381 ctggcgatcg ttttggaacc gcttcgcatc cgcctccaaa acctgacgga tgttctattc
     1441 ttccgtggtt cgcgggtggt ggcagaacag ttggaagact tctctcataa acttaccacc
     1501 gctctagatt tgaatgccat cagttcgctc ctgcgggatc agatcgcctc aaccctcgcc
     1561 ccagaccaga ttcacatctt tacatacgac tccctcaatg atttcttcgt cgcctctccc
     1621 ggcgacggac gccgtactac gactgatatt cgttttacat ctgcaagccc attggtgagt
     1681 tacttcagga gcgaacgctt gccgctgtat ttggacaatg gtaccgacct gcctgatgtg
     1741 cttaagccgg aacagtcgcg tattgcgttg ttgggtgccc gtttgttcat tgcattcccc
     1801 ggtaaggata agccgaatgg ctggctggct ttgggtccac gcctgacgaa tcaggcatat
     1861 tctccaagcg atttgcgctt ccttgaaaat atcagcgacc aagcatccgt tgctattgaa
     1921 cgcgtgcaga cggtggcaca cttggaacgc cgaattcaag aaatgaacgc gttgacccgc
     1981 gtttcacaag gcgtaaacgt tacgctgacc tttgatgatg tcttggaatt gatctatgct
     2041 caaaccgcac agatcgttcc aacttctcac ttccatatca cgctatatga taaagacagt
     2101 gattattact attatggttt ctgtcttgaa aataatgagc gtatgcacga acgcgaaaac
     2161 caacccttct cgtccaatgt tgggctttct ccagatgtta tccgcaagag ccgcccgatc
     2221 attactcaag attacattcg tgaatgtcag gcgcggaatc acactcccac catcgaaaat
     2281 atctttgcat ggatcggtgt gccgcttaac gctggagctg aatcgattgg tgcccttagt
     2341 gttggtagcc gtgatgcctc cacgatctat actcgcgctc aagtagacct attgcaggcg
     2401 attgcagacc aaaccgctgg cgcaatcgtt aaagcgcgtt tgctgaaaga gactcaggaa
     2461 cgtgcgcatc aattgacaac cttgaatgag attacgcgtc agttgacttc gacgcttgaa
     2521 ttgaatccgc ttttgcagaa cattttggaa aacgctgtag gcattttgaa ctgcgaggca
     2581 gggagtttgt tgctggtcga tgatcaaaca gatgaactag ttttccgcgt aacggtagga
     2641 cctgtagctg gcaaccttat cggcaagcgt cttgctcgcg gaacgggcat tgtggggcgg
     2701 gcggttgaaa cgcgcaagcc tgtgattgaa aatgaagagc agacgtcgaa ccgcttcaat
     2761 ggagtagatc aacaaacagg gtttgtgagc cgctcgttac tcgccgttcc actgcaagcg
     2821 aaagaccgtg tgatcggcgt ggtggaagtc atcaaccgct gtgatggtct gccctttgta
     2881 cagaatgacc aaaccttatt aaccgcgttt gcggg
//
LOCUS       DAOREF010000352         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23176_length_2911_cov_4.911765, whole genome shotgun sequence.
ACCESSION   DAOREF010000352 DAOREF010000000
VERSION     DAOREF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23176_length_2911_cov_4.911765"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKI33_08185"
     CDS             <1..335
                     /locus_tag="PKI33_08185"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNM37026.1"
                     /translation="GDGSPTREFLYVEDAADGIITASENYNGDMPVNLGSGYEISIKD
                     LTEMVAKLTGFTGKITWQTDKPNGQPRRGLDVSRAKELFNWSAQVSFEEGMKRTIEWF
                     KANRDKIK"
     gene            387..1232
                     /locus_tag="PKI33_08190"
     CDS             387..1232
                     /locus_tag="PKI33_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37027.1"
                     /translation="MTEITKHEPHTVYIQPSKGLAALNLRDLFMYRELVYFMVWRDVK
                     VKYKQTLLGMAWAVVQPVMTMLVFTFLFDKVAKLPTDGIPYPVFSFAALLPWGLFVNA
                     LNNGSRSLVAHQNMVTKIYFPRLILPTASVFSGMVDFVIAFVILVGLMFYYNVTPAWN
                     LLWTLPLFLLLAIVTALGVALWLSAINVQYRDVNQALPFLSQFWLFATPVAYSASVIS
                     EKWQVVYSLNPMAGVVNGFRWALLGVGNGPDISLWVSVGISILIFVTGLFYFKSMEKT
                     FADTI"
     gene            1232..2524
                     /locus_tag="PKI33_08195"
     CDS             1232..2524
                     /locus_tag="PKI33_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM37028.1"
                     /translation="MTTAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVAAPIRLAK
                     AMIGKSDRRMNQNYVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTEPTTGN
                     VTVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMKRAEIDSKFDEIVDFSEVTQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRT
                     VLFVSHNMSAILRLTQEAIVLNKGQLIMRGPTQEAVDYYLSSGQSQAGERTWDADEVP
                     AASAPFMPISLKVKERGGKVVDTIRSTETVTVEFEYKLSSPVTGLRVGLYVSTMRGEY
                     VFTSFDTDTPALYEKFDSRNAGHYISRAELPADIFNEGRYMVGVNASSFGVRRYFMDE
                     NALAFNVDISGAPGTQWGEPRVGPVRPRLNWKIEKIRE"
     gene            2591..>2911
                     /locus_tag="PKI33_08200"
     CDS             2591..>2911
                     /locus_tag="PKI33_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37029.1"
                     /translation="MPKSNPLREILGDLPFTAEIDWMLRSKNRPRKDHYNLGRLLESL
                     PAAVKVVRPFAEKAPRGKKVLFFATLHYWVEQSAYLGLVLAGLGHDVTLMTLPYSEWH
                     KEKDK"
BASE COUNT          665 a          715 c          780 g          751 t
ORIGIN      
        1 ggggtgatgg ttcccccacc cgtgaatttc tctacgtgga agatgccgcc gatggcatca
       61 ttaccgcctc tgaaaattac aacggcgaca tgcctgtcaa tctcggctcc ggctatgaaa
      121 tttccatcaa agacctcact gagatggtcg ccaaactcac cggatttacg ggcaagatca
      181 cctggcagac agacaaaccc aacggtcagc ctcgccgcgg actggatgtc tcccgtgcga
      241 aagaactctt caattggagc gcacaagttt cctttgaaga aggcatgaag cgcaccattg
      301 agtggttcaa ggcgaatcga gataagatca agtagagaaa gaggagggaa gacagggctt
      361 tttgatcctt cttctttatt ccccctatga ccgaaattac aaaacacgaa cctcatacag
      421 tttatatcca accttccaaa ggacttgccg cgcttaattt gcgggacctg ttcatgtacc
      481 gcgaattggt ctatttcatg gtctggcgcg atgtcaaagt gaaatacaag cagaccttgc
      541 ttggcatggc atgggcggtc gtccaaccgg tgatgaccat gctggtcttc accttccttt
      601 tcgacaaagt ggcgaaactt cccactgacg gtattcccta ccctgtcttt tcgtttgcag
      661 cgttgttgcc gtggggtttg ttcgtcaatg cgctcaacaa tggaagccgc tctcttgtgg
      721 cgcatcagaa catggtcacc aagatttact tcccaaggtt gatcctgccc accgcttctg
      781 ttttctccgg aatggtcgat tttgtcattg cctttgtgat cctggtgggg ctgatgttct
      841 actataacgt tacccccgcc tggaatctgc tctggacgct accgcttttc ctcctgcttg
      901 ctattgtgac cgctctcgga gtagcccttt ggctttcggc gatcaacgtc cagtatcggg
      961 atgtgaacca ggcgcttccg tttctttccc agttctggct gtttgccaca cctgtggctt
     1021 attctgcctc tgtgatctct gagaagtggc aggttgtgta ctctcttaat ccaatggccg
     1081 gtgtggtcaa tggctttcgt tgggcattgc ttggcgttgg caatggacct gatatctccc
     1141 tttgggtttc agtcgggatt tcaattttga tctttgtcac aggcctcttc tacttcaaaa
     1201 gcatggaaaa gacctttgcg gatacgatct aatgacaact gcaatcagtg taaaaaacat
     1261 cggcaagcaa tataagatcg gcgcagcgga gacaaaattc cgctataaca tgctgcgcga
     1321 tgtgatcgtg gacacggttg ccgcgcccat ccgtttggcg aaggcaatga tcggaaaatc
     1381 tgaccgacgc atgaaccaga attatgtttg ggcgttgaag gacgtttctt tcgatctgga
     1441 agaaggtaaa gtgctcggta tcgtcgggcg gaacggtgct ggtaaatcaa ccctcctgaa
     1501 aatcctctcc cgcgtcaccg agccgacgac ggggaatgtc accgtgcgcg gacgggttgg
     1561 ctcgttgctc gaagtaggaa ctggctttca ccccgaattg accggacgtg agaatatcta
     1621 catgaacggc gcgatcctgg ggatgaaacg tgccgagatc gactcgaaat tcgatgagat
     1681 cgtggatttc tctgaagtga cgcagttcat cgacacccct gtgaagcgct attcatcggg
     1741 tatgtatctg cgccttgcat ttgctgttgc tgcacatctc gaaccggaaa tcctcgttgt
     1801 ggatgaagtt cttgctgtgg gtgatgccga gttccaaaag aaatgccttg gtaagatggg
     1861 tgatgtcgca cagcagggtc gtaccgtgct gttcgtcagc cataacatgt ctgcgatttt
     1921 gagattgacc caggaagcga tcgtgctgaa caagggtcaa ctcatcatgc gtggaccgac
     1981 tcaagaggcg gtggattact acctctcttc gggacaatct caagcaggcg aacgcacttg
     2041 ggatgcagat gaggttccag cggcgagtgc gccttttatg ccgatcagcc tcaaggtcaa
     2101 ggaaagaggc gggaaggtcg tcgataccat ccgctctacg gaaacagtca cggttgagtt
     2161 cgagtacaag ttatcctcgc cggttactgg gttacgtgtt ggtttgtatg tcagcaccat
     2221 gcgtggcgaa tatgtcttca cgtcgtttga tacagacaca cccgcgcttt acgaaaaatt
     2281 tgactcgcgc aatgcgggac attacatcag ccgcgcggag ctgcccgccg atattttcaa
     2341 cgaaggtcgt tatatggtgg gtgtgaatgc cagttcgttt ggcgtgcgcc gctattttat
     2401 ggacgaaaat gctctggcgt tcaacgtgga tatttccggt gcgcccggca cgcaatgggg
     2461 cgagccgcgc gttggaccgg tgcgaccgag attgaattgg aagatcgaga agatcagaga
     2521 gtagtgaata gagattggag attagtaatt ggtaattact aatctccaat taccaattac
     2581 taattgccac atgccaaaat caaatccact tcgcgaaatt ttgggcgatc tgcccttcac
     2641 agccgagatc gattggatgc tgcggtcgaa gaatcgtccg cgcaaggatc actataacct
     2701 tgggcgtctg cttgagtcgt tgccagcggc ggtgaaagtg gtgaggccgt ttgcggaaaa
     2761 ggcgccgcgt ggaaaaaaag tcttgttctt cgccacgctt cattattggg tcgagcaatc
     2821 tgcctatctt ggcctggtgc tcgccgggct tgggcatgac gttacattga tgaccctgcc
     2881 ttactcggaa tggcacaaag agaaggataa g
//
LOCUS       DAOREF010000353         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23454_length_2891_cov_3.124118, whole genome shotgun sequence.
ACCESSION   DAOREF010000353 DAOREF010000000
VERSION     DAOREF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23454_length_2891_cov_3.124118"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKI33_08205"
     CDS             <1..882
                     /locus_tag="PKI33_08205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM37030.1"
                     /translation="AAKKFGAELIYTWLPQSAALGAWMGYPVVLEMHADNSGKLGVWW
                     MRQFWKQKTPKVMTVTTSALRKALERSTNFQFPNEALLLAPNGVELEKYAGLPNPVEA
                     RRQLNLKEGLTVGFTGHIYPGRGADLLFELAKQMSAVNFLWVGGTPELVDFWRGKLTE
                     AGMTNVTMTGFVKHESIPLYQAAADVLLMPYSRSIEASSGQDIAEVINPMKMFEYMAA
                     GRGIVCADLGVIREVLNEGNAVFCEPGDVGKWKVEIEKLLADEPRRLALGAQARLDVE
                     QLTWVKREERVIEKLRF"
     gene            complement(871..2700)
                     /locus_tag="PKI33_08210"
     CDS             complement(871..2700)
                     /locus_tag="PKI33_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM37031.1"
                     /translation="MRIERITLTDYPPIKNIDIQISANLVIVAGANGSGKTRLKEAII
                     YPFATAHESQISLLLRSTTPDEESILGQEYLEINQGKISTTLRNYLQTRTGKNAFSGS
                     IIQIESNRTVRPVQFQSITSSTPDPDEQGLDHSLYLDNLSNRWNRLVNNLYAKSAIRN
                     DQLARFIKENPDKTGSDAAKAIPDPLEPFQKTFSNLFPDKKLEPIDIRFLREFEYRDK
                     NSQPLSFGTLSSGERELVTMVFDLIYKEISHSIIIIDEPELHLHPALTFRFIETLKGL
                     CNGTNQIILFTHSADLISTYYTNGNVFVLDPSSTNENQVHILNALNESHSTIANALSE
                     NLGIFAVGKNIVFVEGENSSIDRLIYGKVAQSGFSYFQIVPIGSVENIITLRSVINEL
                     RSTIFGVRLFLIRDRDGLSDKTISTLEENSCFRCLPRRHIENYLLDAEILRDVAISIG
                     MDAEKTSATNIERILQNIASQSLMSGVLWNVREYIRLQGSLSSPKINKGEINQIPPEK
                     LAETITLQLTSSFEELSDKFNSEAILKLTLEEYNKLQESLSSNEWKKYLPGKTIFNRF
                     CSDYFHEKPERIQEIYTNLALQKKPQVFQDISKIFQSFSELES"
BASE COUNT          766 a          514 c          688 g          923 t
ORIGIN      
        1 gctgcgaaga agtttggggc tgagttaatt tacacctggc tgccccaatc cgcggcgttg
       61 ggcgcgtgga tgggctaccc cgttgtcctc gaaatgcacg cggataattc tggcaaattg
      121 ggtgtgtggt ggatgaggca attctggaag cagaagactc ctaaagtaat gacagtcacc
      181 acgtcagcgt tgagaaaggc tttggagcgc tcgaccaact tccagttccc gaatgaagcg
      241 ctgctgctcg ctccgaatgg cgtcgagttg gagaagtatg cgggattgcc aaatccagtc
      301 gaagcgcgtc gccaattaaa tttgaaagag gggctgacgg ttggattcac aggtcatatc
      361 tatcccggca gaggcgcgga tttgttgttt gaacttgcca aacaaatgtc tgcggtgaat
      421 tttttgtggg tgggcggcac gcctgagttg gtggattttt ggcgtggtaa gctgacagaa
      481 gcaggcatga ccaacgtgac gatgactggc ttcgtgaaac atgagtcgat tccgttatat
      541 caagctgcgg cggatgtgtt gttgatgccg tactcgcgtt ccatcgaagc atcgagcgga
      601 caggacattg ctgaggtcat caacccgatg aagatgttcg agtacatggc ggcggggcgg
      661 ggaatcgtgt gcgcggattt gggtgtgatc cgcgaggtgt tgaatgaggg caatgcggtc
      721 ttttgtgagc cgggagatgt tggaaagtgg aaagtggaga ttgagaagtt gcttgctgat
      781 gagccgcgcc gattggcgtt gggtgcgcaa gcccgtttgg atgtagagca gttgacatgg
      841 gtcaagcgcg aagagcgggt gatagagaaa ttaagattct aattctgaga atgactgaaa
      901 gattttagaa atatcttgaa acacttgtgg ttttttctgg agtgctaagt tggtgtaaat
      961 ttcttgaatt ctttcaggct tttcgtggaa atagtcacta caaaacctgt taaaaatagt
     1021 tttcccaggt aaatattttt tccattcgtt tgatgacaat gattcttgca gtttattata
     1081 ttcttcaagc gttaatttta atatagcttc agagttgaat ttatctgaca attcttcaaa
     1141 agagctagtt agttgtagtg taattgtctc tgctaatttt tctggtggaa tttgattgat
     1201 ttctccttta tttatcttcg gcgatgataa ggagccttgt aagcgaatgt attcacgaac
     1261 attccatagg actcctgaca ttaagctttg agaggcaatg ttttgtaata tgcgttcaat
     1321 atttgttgca cttgtctttt cagcatccat gccaatactt attgcaacat cccttaatat
     1381 ctcagcatct agtaagtagt tttcaatatg ccgtctggga aggcatcgaa aacaagaatt
     1441 ttcttctagt gtcgaaattg ttttatctga taaaccatcg cggtctctta tgagaaataa
     1501 ccttacacca aaaatcgtac ttcttagttc attaatgaca gatcttaaag tgataatatt
     1561 ttcaacagaa ccaataggaa caatttgaaa gtacgaaaac ccgctttgtg caactttgcc
     1621 gtaaataagg cggtcaatgc ttgagttctc cccttctacg aaaactatat tttttccaac
     1681 agcaaatatt ccgaggtttt cacttaaggc attagcaatt gttgaatggc tttcatttaa
     1741 cgcatttaaa atgtgaactt gattctcatt tgtagaactt gggtcaagta caaacacatt
     1801 tccattagta tagtaagtag atattaagtc tgccgagtga gtgaatagaa ttatttgatt
     1861 cgttccattg catagtcctt tgagagtttc aataaatcga aaggtcaatg ctggatgtag
     1921 gtgcaattct ggttcgtcaa taataattat agagtgggaa atttctttat atatcaagtc
     1981 aaaaaccatt gtaactaatt cccgctcccc tgaactaaga gtaccaaaag aaagtggttg
     2041 agaatttttg tctctatatt caaattctct gaggaatctt atatctatcg gttcaagctt
     2101 tttgtcaggg aataggttgc tgaatgtttt ttgaaaaggt tctaaagggt ctggaatggc
     2161 tttagcggca tcggaaccgg ttttgtcagg gttttctttt ataaatcttg cgagttgatc
     2221 gtttcgtatt gctgattttg cgtaaagatt atttactaac ctattccaac gattggaaag
     2281 gttatctaaa tataaagaat gatctaaacc ttgctcgtca ggatctggcg tgcttgaagt
     2341 aatgctttga aattggactg gacgtacggt tctgttagat tcgatctgaa taatagaacc
     2401 agaaaaggcg ttttttccag tgcgagtttg taaataattt cgaagtgtgg tcgagatttt
     2461 cccttgattg atttctagat actcttgacc taaaatgctt tcttcatcag gagtagtgct
     2521 tcgtaataat aacgaaatct gagattcgtg ggcagttgca aatggataaa taatagcttc
     2581 ttttaggcga gtttttccag aaccatttgc accagcaaca attacgaggt ttgctgaaat
     2641 ttgaatgtca atattcttga ttggagggta atcagtaaga gtaattcttt caatgcgcat
     2701 tttgctactc cctcgttgtg ggtgaaaggg ctttcttttt actttctaaa ttatagcctt
     2761 attccttcgt caccgccgtc aaattcattg tgagcaatac tccaggtttg ggaagttcgt
     2821 tcacaccgat gaccgtccgc gcggggcgat ggtctcccat tttgctgacg tgagcgcggt
     2881 tcatttcttc a
//
LOCUS       DAOREF010000354         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23473_length_2890_cov_3.162257, whole genome shotgun sequence.
ACCESSION   DAOREF010000354 DAOREF010000000
VERSION     DAOREF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23473_length_2890_cov_3.162257"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKI33_08215"
     CDS             complement(<1..362)
                     /locus_tag="PKI33_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37032.1"
                     /translation="MLPTSQKQTQFHTYRLEYLDHFNSPELDRSKWIPYYLPQWSSRA
                     KTQPRYRMEESCLILEIQPDQQPWSPEFNGSIRVSSLQTGLYSGPLHSTEGQHRFSKD
                     LRVREAQPTERTYTPQYG"
     gene            complement(363..689)
                     /locus_tag="PKI33_08220"
     CDS             complement(363..689)
                     /locus_tag="PKI33_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37033.1"
                     /translation="MKINGIEGMTVSHVQSEVARGGKFVMYQYCISVLIMTFRRNSGI
                     YFIKAGESAVTPGLGFTLLSLVLGWWGIPWGPIYTIGSIYTNFNGGKDVTQQIMASIA
                     RPTPQA"
     gene            complement(686..1045)
                     /locus_tag="PKI33_08225"
     CDS             complement(686..1045)
                     /locus_tag="PKI33_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37034.1"
                     /translation="MIRIISLKTLGIIIAFTQLFDLVLHAITNQLEFLRVTSNIIILL
                     WLVLFFFGNMKISYFASFGTIGLYLFFNILFLAQAGLTNPAQGGAPRTLLFILVFLTI
                     VLSTLFTLHHQRSGQIS"
     gene            complement(1050..1502)
                     /locus_tag="PKI33_08230"
     CDS             complement(1050..1502)
                     /locus_tag="PKI33_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37035.1"
                     /translation="MKPIQFEIHGVTSKSSEEICAALLDTERWSDFGGYSILPGIKHA
                     HFEIRTPEVIGSRIIVQNTDGSAHIEEIVEWDPAKKVVLKFQAFTPPLKNIASHFIET
                     WEFERINTETHTKRSMSMYPNHLFGWLILLPISRLMKKAFEQNAKQFS"
     gene            complement(1503..1928)
                     /locus_tag="PKI33_08235"
     CDS             complement(1503..1928)
                     /locus_tag="PKI33_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNM37036.1"
                     /translation="MHIPQSLIDLVESGAHAHLVTINKDGTPHVTVVWIGVENDEIVC
                     AHLPRNQKVRNIERNGNVILSLEADTKSSYGLKEYAVVHGTARITEGGAPELLQRLAY
                     VYMGPGVKFPPMDNPPPGYITRINVTRIEGVGPWVSKGE"
     gene            complement(1957..>2890)
                     /locus_tag="PKI33_08240"
     CDS             complement(1957..>2890)
                     /locus_tag="PKI33_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNM37037.1"
                     /translation="KDIPSDSKIDVQIGPEWPAEVVAIKYLSSADNTLQPAIFYRPNT
                     TDRVPLLVALHTWTGDYLQILNTPLAQWCIENNWAFIHPNFRGPNNKFEAIGSDLVIE
                     DILSAVKFAETYSNIDTHNIYLVGASGGGHAALMVAATHPNVWAGVSVWVPITDLSVW
                     YEETKTLDGQYRIFLEQALGGAPGTSSAIDLEYKKRSPLTYLSPSIHLNLDINAGIHD
                     GHNGPVPISHSLLAYSALVTAEDQLSAKDIKYFVEQEKVPQHLIDPSLSDSSYGEKRP
                     IFRAQSGTTRLTIFEGGHELVIDAVINWLTQSLK"
BASE COUNT          771 a          591 c          687 g          841 t
ORIGIN      
        1 tagccatatt gcggagtata cgtccgctcg gtgggttggg cttcgcgcac gcgtaaatct
       61 tttgagaagc ggtgctgacc ttcagttgaa tgcaatggac ctgaatagag tcccgtttgc
      121 aatgaagaga cgcgaatact tccattgaat tcaggcgacc agggctgttg gtcaggttga
      181 atttcaagga tgaggcaact ctcttccatg cgatagcgcg gctgggtctt tgcgcgcgaa
      241 ctccactgcg gcaggtagta gggaatccac ttggagcggt ctaactctgg cgaattgaaa
      301 tgatcgagat attccaaacg ataggtgtgg aattgagttt gtttttgcga agttggcaac
      361 atttatgctt gtggtgtggg gcgagcaatg gatgccatta tctgttgggt cacatctttg
      421 ccgccgttaa aatttgtgta tatagagccg atggtgtaga tcggtcccca agggatgccc
      481 caccagccaa gcaccaacga cagtagggta aatcccagcc ctggggtaac cgcactttcg
      541 ccagctttga tgaaataaat tccagaattt ctgcgaaagg tcatgatcag taccgagata
      601 cagtattgat acatgacgaa ttttccgcct cgcgctactt cagactgaac atgggaaact
      661 gtcatacctt cgatgccgtt gattttcatg agatttgtcc gctcctttgg tggtgtaggg
      721 tgaatagggt tgataacaca atggttagaa ataccaaaat gaatagtagg gtgcgcggtg
      781 cacctccctg tgcggggttg gtcaacccgg cttgagcgag gaaaaggata ttgaaaaaca
      841 gatacaagcc aatagtcccg aaagaggcga agtaacttat cttcatgttt ccaaagaaaa
      901 atagcaccag ccagagcaag atgataatgt tcgacgtgac tcgtaaaaat tctaattgat
      961 tggttatggc atgaagaaca aggtcgaaga gttgcgtaaa ggctatgata attcccagtg
     1021 ttttgagtga aattattcgt atcatgtgtt taggaaaatt gcttggcatt ttgctcaaag
     1081 gcttttttca ttagccttga aataggcaat aaaatcagcc aaccgaatag atgatttggg
     1141 tacatgctca tgcttcgttt cgtatgggtt tctgtattga ttctctcgaa ttcccaagtc
     1201 tcaataaaat gtgaagcgat atttttcaat gggggagtga aggcttgaaa tttaagaacg
     1261 acttttttcg caggatccca ttccacgatt tcttcaatgt gcgcggaacc atctgtattt
     1321 tgaacgatga ttcttgaacc gatcacttcg ggggtacgaa tttcgaaatg ggcatgtttg
     1381 atgcccggca agatcgaata tcctccaaag tcagaccatc gttcggtgtc taaaagcgca
     1441 gcgcaaatct cttctgatga ctttgaagta acaccatgaa tctcaaattg aataggcttc
     1501 atctattctc ctttgctcac ccacggtccc acaccttcaa tgcgagtgac gttgatcctt
     1561 gtgatatagc cgggaggggg attgtccatt ggggggaatt tcacgccggg acccatatac
     1621 acataggcaa gtctttgcaa caactccggt gcgcctcctt ctgtaattcg agcggttccg
     1681 tggaccacgg catattcctt caacccatag ctacttttgg tatccgcttc caatgagagg
     1741 atcacattcc cattccgctc aatgttacgc actttctgat tgcgcggcaa atgagcacaa
     1801 acgatctcat cattttcgac gccgatccat accacggtga catgtggtgt gccgtctttg
     1861 ttgattgtta ccagatgtgc atgggcacca gattcgacca agtctatgag agattgaggg
     1921 atatgcatgg ggtggctcct gagtatagaa aaatacttat ttaagacttt gtgttaacca
     1981 gtttatcact gcatctatta cgagttcatg cccaccttca aatatagtga gacgagttgt
     2041 tccagattgt gctctaaaga tcgggcgctt ttctccatat gaggagtctg acaatgaagg
     2101 gtctattaga tgttgcggca ctttttcttg ctcaacaaaa tattttatgt ctttagcaga
     2161 taactgatcc tctgctgtaa ctagagcgct gtatgctagt agtgagtggc taatgggcac
     2221 tggtccatta tgtccatcgt gaattcctgc attaatgtct aggtttaagt ggattgatgg
     2281 agataaatat gtgagaggag agcgtttctt gtactctaaa tctattgcgg atgacgtgcc
     2341 gggggcgcct ccgagagcct gctctaaaaa tattctgtat tgtccatcta atgttttggt
     2401 ttcttcatac catacggata ggtctgtaat cggaacccat actgaaacac cagcccaaac
     2461 attggggtgg gtggcagcca ccataagagc tgcatgcccg cctcctgatg ctcctactaa
     2521 ataaatatta tgagtgtcaa tatttgaata cgtttctgca aacttaactg cacttaaaat
     2581 gtcttcaata accaaatctg aaccaatggc ttcaaattta ttgtttggac ctcggaaatt
     2641 tggatgaatg aaagcccaat tattctcaat acaccattgt gcaagaggag tattgagaat
     2701 ttgaagataa tcaccagtcc atgtgtgtag tgctactagt aaaggaacac gatcggttgt
     2761 atttggtcta taaaaaatag caggttgaag tgtgttatct gcagaactta gatatttgat
     2821 tgcaactact tctgcgggcc attcaggtcc aatttgtaca tcgatttttg agtcagatgg
     2881 gatatcttta
//
LOCUS       DAOREF010000355         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23484_length_2889_cov_3.232534, whole genome shotgun sequence.
ACCESSION   DAOREF010000355 DAOREF010000000
VERSION     DAOREF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23484_length_2889_cov_3.232534"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PKI33_08245"
     CDS             complement(<1..813)
                     /locus_tag="PKI33_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNM37038.1"
                     /translation="MQKATHQQTKQHNRDLVLRTIFANESISRAEVARVTNLTRTTVS
                     DVVNSLLDEGLVEEVGRGESIGGKTPILLSVVADSRYLIGLNLAQDKFIGAIVNLRGE
                     IKEMVEVPVHGEDGKNALDLVYQIIDQLMKKRIKPIVGIGVGAPGLVNTREGVVVNAV
                     NLEWQDLPLGQLLEKKYKIPVLILNDSQATAIGEFVYGGAHALDENLIVVNVKHGIGS
                     GILVNGRLFQGDGGGAGEIGHVVVQENGELCRCGKHGCLETISSARAVLRQLN"
     gene            1010..1699
                     /locus_tag="PKI33_08250"
     CDS             1010..1699
                     /locus_tag="PKI33_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37039.1"
                     /translation="MELIPFNLIGILGIVGATTYAVGDVLLLASKISLDDYPKLKPFA
                     KLLSDSEKMVALSPIRMMWGALLGVFATPLVLFGFWHAYQGLSGVNAWVALIAFFLFA
                     LASIIGTFVHGTFYYMGEYVHALNKVSTDSQAVIVEMIERHKKVLIITYAPLLVFIIF
                     ASILFSILVASGNTLFPVWMTAINPLTLTILWMLGKRILPQFVRDWTEGAGFNIAYMA
                     FFACTTITLWK"
     gene            1703..2365
                     /locus_tag="PKI33_08255"
     CDS             1703..2365
                     /locus_tag="PKI33_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNM37040.1"
                     /translation="MKTSTSIQDYLKRIYELTESGQSASTNDLARELKVKPASVTGMI
                     QKLAAETPALVEYQKHQGVTLTVAGKRAALEVIRHHRLLETWLVQTLGYSWDEVYEEA
                     ERLEHVISEDFERRIAAALGHPLRDPHGELIPTEDLKMPLDESTPLSALRKDQSGTIL
                     CIKAADADLLRHLTELGLTPNTFIQVTEYSPYDHNLTIRVDKKTHVLGLSITSKIFIE
                     EG"
     gene            2426..>2889
                     /locus_tag="PKI33_08260"
     CDS             2426..>2889
                     /locus_tag="PKI33_08260"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNM37041.1"
                     /translation="MNETIFRILAAVILFTGVGISIYFRSKADRTSGEKLSRNVDGTP
                     LMLVIRIFGLVLWLSPLVYLVNPVWMNWSKISLPAWSRWFGVVLGIFCVFGIYWLFTS
                     IGSGITPVSATRKEHKLATSGIYRWIRHPLYTVGSSFIVSFGLMADNWFIIA"
BASE COUNT          772 a          710 c          612 g          795 t
ORIGIN      
        1 attcaattgg cgaagtactg cacgagcgct ggaaatggtt tcaagacagc catgttttcc
       61 acaacgacat aattcgccat tctcttgcac caccacatga ccaatttcgc ccgccccgcc
      121 cccatcgccc tggaacaaac gaccgttgac taaaatacca gagccaatgc catgtttcac
      181 attgactaca atcaagtttt catccaaagc atgcgcgcca ccatagacaa actcaccaat
      241 agcggttgcc tggctgtcat ttaagatcaa aacagggatc ttgtatttct tttccagcaa
      301 ttgaccaagt ggcaaatctt gccattccaa attgacggca ttaaccacca caccttcgcg
      361 ggtgttgacc aagcccggag cgcccactcc aattccaacg atgggcttga tgcgtttctt
      421 catcaattga tcaataattt gataaacgag gtcaagggca ttcttgccat cttccccgtg
      481 aacgggaact tcgaccattt ctttgatttc cccacgtaaa tttacgatcg cgccaatgaa
      541 tttatcctgt gcgagattca aaccaatcag ataacgcgaa tctgctacaa cactaagcag
      601 gatcggtgtt ttgccaccaa tcgattcgcc tctgccaact tcttcgacca agccttcatc
      661 caacaaactg ttgactacat ctgaaaccgt ggtgcgtgtg aggttggtaa cacgcgccac
      721 ttctgcacgg ctgatagatt cgtttgcgaa gatggttcgc agaacgagat cacgattgtg
      781 ttgcttggtt tgctgatgag tcgctttttg catgattttt gccaaaattt ctacgcgcag
      841 aagtatcttg gatagcaaac gatcatttaa ttactttgta aattcactgt actaacaaag
      901 ttagtataca gatttccgca gcaagagtca aggctgaatc tattgacatt ttttagggga
      961 tattataaaa taatttaggt aagactaaat tattttataa acgaaaatta tggaacttat
     1021 tccctttaac ttaattggta ttctgggcat cgttggtgcg accacctatg cagttggtga
     1081 tgtgctttta cttgcctcaa aaatctccct cgacgactac ccaaaactca agccatttgc
     1141 caaactactt tctgactcag aaaaaatggt ggcactctcc cccatccgca tgatgtgggg
     1201 cgcattactt ggagtgtttg caactcccct cgtactattt gggttttggc atgcatatca
     1261 aggcttaagc ggagtcaacg cctgggtagc attaatcgct tttttccttt ttgcattggc
     1321 ttccatcatc ggaacctttg tacatggaac gttttactac atgggcgagt atgtgcatgc
     1381 cttgaacaaa gtctccactg actcgcaagc agtaatcgta gaaatgattg aaagacacaa
     1441 aaaagttctt atcatcacgt atgccccgtt gttggtgttc attattttcg cttctatcct
     1501 attttcgatt ttggtcgctt ctgggaatac tttattccca gtatggatga cagcgatcaa
     1561 cccacttacc ctcacgattc tttggatgct cggaaagcgc attctgccgc aattcgtccg
     1621 cgattggacg gagggtgccg gtttcaacat cgcatatatg gcattctttg cctgcacaac
     1681 tatcactctt tggaaataat caatgaaaac atccacatcc atacaagatt atctcaaacg
     1741 catctacgaa ctgaccgaaa gcgggcaatc agccagcacc aatgatctgg cacgtgagct
     1801 aaaagtcaag ccagcatctg tcaccggtat gatccaaaaa cttgcagcag aaacgcctgc
     1861 cttggtcgaa taccaaaagc atcagggcgt cactctcacc gtggctggca aacgtgccgc
     1921 tctggaagtg atccgccatc atcgtttgct ggaaacctgg ctcgtacaaa cccttgggta
     1981 ttcatgggat gaagtctatg aagaggcaga acgattggag catgtcattt cagaggattt
     2041 cgagcgacgc atcgctgccg ctttggggca tccgcttcgt gacccgcacg gagagttgat
     2101 ccccaccgaa gatcttaaga tgccgctgga tgagtccact ccgctttctg ctttgcgaaa
     2161 agaccaatct ggcacgatct tatgcatcaa agccgcagac gcagacctgc ttcgccatct
     2221 gaccgagctg gggcttacac caaacacgtt cattcaggtg acagaatatt ccccatacga
     2281 ccacaacctt accattcggg ttgataaaaa gacacatgtg ttggggttga gtatcactag
     2341 caagatattc attgaggaag gttaatcgac aaagttatcg aataaatgaa aagtaagaaa
     2401 cagaaagaag aaaggcttta acactatgaa cgaaaccatt tttagaatct tggcagccgt
     2461 tatccttttt acaggtgtag gcatctccat atatttccgt agcaaggcag accgtacttc
     2521 aggagaaaaa ctatctcgca atgtagacgg cactccgctt atgctggtca ttcgcatctt
     2581 tgggttggtg ctctggctca gtccgctcgt ctacttagtc aaccctgtat ggatgaactg
     2641 gtcgaagatc agtttacccg catggagccg ttggttcgga gtcgtgctcg gtatattttg
     2701 cgtcttcgga atttactggt tgttcaccag tattggcagc ggaattacac cggtcagtgc
     2761 aacacgcaaa gagcataaac ttgcaaccag tggcatctac cgctggataa ggcatccact
     2821 ttataccgtg ggctcatcat ttatcgtctc gtttggtctc atggcagata actggttcat
     2881 tattgcatt
//
LOCUS       DAOREF010000356         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23538_length_2885_cov_4.234982, whole genome shotgun sequence.
ACCESSION   DAOREF010000356 DAOREF010000000
VERSION     DAOREF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_2885_cov_4.234982"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKI33_08265"
     CDS             complement(<1..295)
                     /locus_tag="PKI33_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNM37042.1"
                     /translation="MTANFKPNVLKKRYALRLFVAGNATNSRIARENLEQLLARHPEH
                     EFEVEIVDLNVQPEFALDQGVFISPALQILEPSSGGIVYGNLSQKEVLEKVLNL"
     gene            complement(285..1712)
                     /gene="kaiC"
                     /locus_tag="PKI33_08270"
     CDS             complement(285..1712)
                     /gene="kaiC"
                     /locus_tag="PKI33_08270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNM37043.1"
                     /translation="MATQTLKTPTKIKGLDEVLNGGVPTGGLTLLNGGPGTGKTLFGL
                     EFLVRGAQEGIPGILITFEEHEEALHHYATTFGWNTAMLEKQGLLSIIGARFDPEGLL
                     SGDFDLGGILAILRHKTEKLSAKQVVIDAPDVFLRLLDNRGKERAELYKLINWLRDEK
                     LTALMTVKADGGDVARSDYEFLDYLADCVIQLDQRVIEQVTTRRVRVVKYRGSAHGRN
                     EYPFAMTDQGIWIIPITETQLNHAALGAPFPTGVAGLDAYMSGGYLRASCNLVTGTSG
                     TGKTTFAASFARTSAANGKRVFYINFEESPDSMVSCMLSPGIDLRPAMEDGSLRFLSV
                     MPEAQGIEEHLIQAFRGIEELKPDIVIVDAISACRRMGSRNSAFDYLLRLIDHCKTIG
                     ITSLLTNLTDSVKETQEITGIDLSSMIDTVVILRNVEYNGQFQRELSVLKARGRQHSN
                     KVHKFQITDHGVSIVNSNIEDAHDR"
     gene            complement(2028..2735)
                     /locus_tag="PKI33_08275"
     CDS             complement(2028..2735)
                     /locus_tag="PKI33_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37044.1"
                     /translation="MLERLDLTQALEREEYNKQLKALQAEVHELAFQVYAQKRPVVIV
                     YEGMDASGKGGTIKRLTERIDPRGYVVWPIAAPSGEDKERHYLYRFWRRLPEQGQIAI
                     FDRSWYGRVLVERLEGYCTEADWRRAYGEINQFERQLTDYGTIVFKFWMHISKQEQLR
                     RFRERTRTNYKAWKITDEDWRNRGKWEDYIIAAEEMLLKTSTPDSPWTLVEANDKYFA
                     RIKSLRTVAEKLRRALK"
BASE COUNT          718 a          714 c          681 g          772 t
ORIGIN      
        1 aaaggttcaa gactttttcc aaaacttctt tttgacttaa gttgccgtac acaatcccac
       61 cagaacttgg ttcaaggatt tgtaaggcgg gggatataaa cacaccctga tccaaggcaa
      121 attcgggttg tacgttgagg tcaacaattt caacttcaaa ttcatgctca gggtgtcttg
      181 ccaacaactg ctctaagttt tcacgcgcga tccgtgaatt ggtggcattc ccggcaacaa
      241 acaagcgcag ggcataacgt tttttaagaa cgttaggttt gaaattagcg gtcatgggca
      301 tcctctatat ttgaattcac tatagaaacc ccatgatcag taatctgaaa cttatggact
      361 ttatttgaat gttgacgtcc tcgggcttta agcacagaca gttctcgttg gaattgcccg
      421 ttgtattcta cattgcgcaa aataactaca gtgtcaatca tggaagacag gtcaatgcca
      481 gtgatttcct gagtttcttt tacactatcg gttaggttgg tcaacaggct ggtaatacca
      541 atagtcttac agtgatcgat cagacgcaac aaataatcga aagcagaatt acgtgacccc
      601 atgcgtcgac aagccgagat ggcatctacg atcacgatat cgggtttcaa ttcctcaatc
      661 ccgcgaaatg cttgaataag atgctcttcg attccctgcg cctcaggcat cacagataag
      721 aacctcagag aaccatcttc catagctggg cgcagatcta taccggggct aagcatgcaa
      781 gataccattg agtctggtga ttcttcaaag ttgatgtaaa agacgcgttt gccattggca
      841 gcggatgtac gcgcaaacga agcggcaaag gttgtcttac ccgtaccaga tgtacctgtg
      901 accaaattgc aggaagcacg cagataccca ccgctcatgt atgcatccaa accagccaca
      961 ccggtgggga acggcgcacc caacgccgca tggtttagtt gtgtttccgt gatcggaatg
     1021 atccaaatgc cttgatcggt catggcaaac gggtactcat tccgtccatg tgcagagccg
     1081 cgatacttga ccacacgtac ccgacgggtg gtgacttgtt cgatcacacg ttgatcaagt
     1141 tggatcacac aatctgcaag ataatcaaga aactcataat cagaacgggc aacatccccc
     1201 ccatctgctt tgaccgtcat cagagcagtc aacttttcgt cacgcaacca gttgattagt
     1261 ttatataact ccgcacgctc cttgccacga ttatcaagca aacggagaaa tacatcggga
     1321 gcatcaataa caacctgctt agctgaaagc ttttctgttt tgtggcgcaa gatcgccaga
     1381 atgccgccca agtcaaaatc gcctgagagt aacccctccg ggtcgaacct tgccccaatg
     1441 atcgaaagaa gtccttgttt ttcaagcata gcagtattcc agccaaatgt ggtggcgtaa
     1501 tgatgcaagg cttcttcatg ttcttcaaac gtaattagga tgccaggaat cccctcttgt
     1561 gcgcctcgca ccaggaactc tagcccaaac aaagttttgc cggtgccggg tcctccgttc
     1621 aacaaggtca atccgccagt gggtacacct ccattaagga cttcatcgag accttttatt
     1681 ttggtaggag ttttaagggt ttgggttgcc ataaaatcag cattcctttc aagagaatag
     1741 gatctggtta tgtctcaaac aaggctagtc tgcttgccta acgtatggaa ctatttcatt
     1801 gcaaatatga cattacactt taatatgatc atctctcgat accgatttca tcgtctcccc
     1861 gcaatcctaa ccgcacaaac gttaaaaggc taaatttatt ttatacacat ttttgcattt
     1921 ttacacatgc ttttcattat gatttgaggg tgttttcctc ccatttctcg gaaaatagtg
     1981 cgtaaaacag gcatccgata gggtacggct catgagggca tagcaattca cttcaacgcg
     2041 cgccgtaatt tctcggcgac cgtgcgcagg gacttaatgc gggcaaagta tttgtcgttg
     2101 gcttccacca atgtccaggg ggagtcgggt gtgctggtct tcaagagcat ctcttctgcc
     2161 gcgatgatgt aatcttccca tttgccgcgg ttgcgccaat cttcatctgt gatcttccag
     2221 gctttgtaat tcgtgcgcgt gcgttcgcgg aagcggcgca attgttcctg cttgctgatg
     2281 tgcatccaga atttgaagac gatggtgccg taatcggtaa gttgacgctc gaattggttg
     2341 atctcgccat aggcgcggcg ccaatcggct tctgtgcaat agccttcaag gcgttccacc
     2401 agtacacgcc cataccagga acggtcgaag atggcgatct ggccttgttc gggcaatcgc
     2461 cgccagaaac gatacaggta atgccgttct ttgtcttcgc cagagggggc ggcgatgggc
     2521 cagaccacgt atccgcgtgg atcgatgcgc tcggtcaggc gtttgatcgt gccgcccttg
     2581 cctgaagcat ccatgccttc atagacgatc accacagggc gtttctgggc atagacctga
     2641 aacgccagct catgtacttc tgcctgcaat gccttgagtt gtttgttgta ttcctcgcgt
     2701 tcaagggctt gggtaagatc gagtcgctca agcattgacg gctcctttca tttcctttgt
     2761 tttgcgcgga cgtttggact ttgcccctgt tgaggagggc agtcccagac gggcttcgag
     2821 cgaggcgatg atggtctcga agactttgat gcgcatgtaa tatcggtcgg tggcttcgac
     2881 aattg
//
LOCUS       DAOREF010000357         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23712_length_2874_cov_5.003547, whole genome shotgun sequence.
ACCESSION   DAOREF010000357 DAOREF010000000
VERSION     DAOREF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23712_length_2874_cov_5.003547"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKI33_08280"
     CDS             <1..978
                     /locus_tag="PKI33_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37045.1"
                     /translation="IFLFACLALTPPTEEPAPPTEETTAPENTTIVPEQPIGNGPSIE
                     NCPMFPENNVWNTPIDSLPLHPLSDAWIESIGSAEPIHMDFGSGTWDGGLIGIPFNIV
                     AGSAVPTFEPGFYYPEESDAGPYPIPDNPSIEYGSDHHILVVDTETCTLYEIFDASFD
                     GSNWNGGGGAIWDLNSNALRPDTWTSADAAGLPILPGLVRYDEIVAGEIKHALRFTAE
                     QTAGYLWPGRHLTADPDDSIPPLGARFRLKADYDISGFPPEMQVILQALKTYGMFLAD
                     NGSDWYVSGAPDERWDNDMLHLLDVLVGSDFEVVDASGMIVDVDSGEAK"
     gene            1013..1351
                     /locus_tag="PKI33_08285"
     CDS             1013..1351
                     /locus_tag="PKI33_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37046.1"
                     /translation="MSNSVHETIPQNRKGRWGRFGLAISILSQVVLLCSIISFFSSFQ
                     FMEGMFVPILFVSLPLAVIAIFLIVIGLVLCIVGIFTQDGSRKTALIGLGVLSFTFVF
                     LLIAYLRLNP"
     gene            1393..2727
                     /locus_tag="PKI33_08290"
     CDS             1393..2727
                     /locus_tag="PKI33_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37047.1"
                     /translation="MTIPQPSTNDTKNKKKYFTTLAFIFNAMGILLALLIIPAVYLSY
                     IPFAILITAPAMLIIGVLAIILWAIGLYQGIKAIRSEETRKIGAWVATLLNGFLIIGA
                     TCSFIVATSLIFSPTTSIFSTYYSGAGFSPDGNVVAVGYKNKILLLDSKTGEKIKTLG
                     GNKNATDLIIFSPDGNLLADSSYGRLTIRDMTTYKTLKTFTKENDRVFDFYFSPDGKL
                     ITIIWFNKGAEVISTSTFEVLKTVSFGNNFDTVYNKYISETSHHFGKTDNSEENLSVW
                     EMNDFIENSLTKPIISIPLPQSSENTFVYFDRYDVTPDGSLLVVPIPTSIDGDYNKSE
                     FILWNITTAELIATLEFNPVIGIGGYDSHPDPEYYFAFSPDSQTLAYINEKNNITLID
                     LNTFQTRDIDVEDEYVARYLAYNHDGSQILITNAKSIQMISVDSGNIVWTYP"
BASE COUNT          779 a          647 c          621 g          827 t
ORIGIN      
        1 atttttcttt ttgcatgtct tgcgctcaca ccgcccactg aagaacctgc tccgcctaca
       61 gaagaaacca ctgccccaga aaatacaact atcgtccctg aacaacccat aggaaatggt
      121 ccaagcattg agaattgccc aatgttccct gagaataatg tatggaacac gccgatcgat
      181 tcgcttccgc ttcacccgtt gtctgatgcg tggatcgaga gcattggctc agccgaaccc
      241 attcacatgg acttcggctc agggacgtgg gatggaggtt tgattggaat cccattcaat
      301 atcgtggcag ggtcagccgt cccgacgttt gagccggggt tttattatcc tgaagaatca
      361 gatgcaggtc cgtaccccat ccctgataat ccaagcatcg agtatggctc cgaccatcat
      421 attctggttg tagatacaga gacctgcacc ttgtatgaga tctttgacgc cagcttcgac
      481 ggctcaaatt ggaatggcgg cggtggcgcg atctgggact tgaattcaaa tgccctgcgc
      541 cccgatacat ggacctctgc cgacgcggcg gggttgccaa ttttgccggg cttagtgcgc
      601 tatgacgaaa ttgtagcagg cgagatcaaa cacgccctgc gtttcactgc cgaacagacc
      661 gctggctatc tctggcccgg tcgtcacttg acggctgacc cagatgatag catccctccg
      721 ctgggtgcgc gtttccgttt gaaagccgat tacgatatta gcggcttccc tcctgaaatg
      781 caggtcatct tacaggcgtt gaaaacctac ggcatgttcc tggcagataa cggttcagac
      841 tggtatgtga gcggcgcccc cgatgagcgc tgggacaatg acatgctgca tctgttagat
      901 gttttggtcg ggagtgattt tgaggtggtg gatgcttcag gcatgatcgt ggatgtggat
      961 tcaggagagg caaaatagaa aaaggctttg aaagcctgga ggaatattta acatgagcaa
     1021 ctcagtacat gaaacaatac ctcagaaccg aaaaggacgg tggggaagat ttgggcttgc
     1081 aattagtatt ttgagtcagg tggtattgct atgttcaatt atttctttct tttccagctt
     1141 ccaatttatg gaaggcatgt tcgtgccaat tctattcgtt agtttacctc tggcagtgat
     1201 tgcaattttc ctgatcgtta tcgggctggt cctatgtatc gtcggaattt ttacacaaga
     1261 tgggtccaga aagaccgccc tcattgggtt gggcgtatta tcgtttacct tcgtgtttct
     1321 gttgatcgca tatctacgtc ttaatccata atagttttat taattacatt tatgtgaaag
     1381 gtgactttaa taatgactat tcctcaacct tcaacgaacg ataccaaaaa caaaaagaag
     1441 tatttcacta ccctggcttt tattttcaat gcaatgggaa ttctgcttgc ccttcttatc
     1501 atccctgcgg tatatctcag ctacataccg tttgccattc tcataacagc tccagccatg
     1561 cttatcattg gagttttggc tatcattctt tgggcaattg gcttatacca gggcataaaa
     1621 gcaattcgtt ctgaagaaac tcgtaaaatc ggcgcatggg ttgcgacttt actaaatggc
     1681 ttcctcataa ttggcgcaac atgtagtttc atagtagcca catcactgat tttctcgccg
     1741 accacttcaa ttttctcaac atattatagt ggtgcaggct tttcacctga tgggaatgta
     1801 gttgcggttg gatataaaaa taaaatccta ttattagact ctaagaccgg cgaaaaaata
     1861 aaaacattgg gtggcaacaa aaacgctact gacttgatca tcttttcacc agatgggaat
     1921 ttactagctg attcctcgta tggtaggttg accattcgtg atatgacgac ctataagacc
     1981 cttaagacct ttacaaaaga aaatgatagg gtattcgatt tttatttttc gccagatggg
     2041 aaactcataa ccattatttg gtttaataaa ggtgctgaag taattagcac atctactttt
     2101 gaggtcttaa aaactgtttc cttcgggaat aattttgata ctgtttataa caaatatatt
     2161 tcagagacaa gccatcactt tggcaagaca gataatagtg aagaaaacct atccgtttgg
     2221 gaaatgaacg atttcatcga aaactcacta acaaaaccta tcatctctat tcctctgcct
     2281 caaagttctg aaaacacttt tgtttacttt gaccgctatg acgttactcc tgatgggtca
     2341 ctcttggtgg tgccaattcc aacatccatt gacggcgact acaataaatc tgagtttata
     2401 ttgtggaata tcactaccgc tgaattgatc gcaacgctag aattcaatcc agtaataggg
     2461 ataggcgggt atgattctca cccagaccct gaatactatt tcgctttttc accagatagc
     2521 caaactcttg cgtatattaa cgagaaaaat aatataacct taatcgatct aaatacattt
     2581 caaaccagag atatcgatgt tgaagacgaa tacgtggcgc gatatttggc atacaaccat
     2641 gatggaagtc aaatccttat tacaaatgcc aaatctattc aaatgataag cgttgactct
     2701 ggtaatatcg tatggacgta tccttaatta ctaaatgaat tccttcaccc cgcgcccctc
     2761 tcaacaaaag atcctccgct ataacggcgg gaggcttggt attgctgccg tgcccggtgc
     2821 aggtaagacg cacattctct cggcacttgc cgccaagctc attcaagaag ggcg
//
LOCUS       DAOREF010000358         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23760_length_2871_cov_3.931108, whole genome shotgun sequence.
ACCESSION   DAOREF010000358 DAOREF010000000
VERSION     DAOREF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23760_length_2871_cov_3.931108"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..954)
                     /locus_tag="PKI33_08295"
     CDS             complement(67..954)
                     /locus_tag="PKI33_08295"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNM37048.1"
                     /translation="MEDRIITYGGQAVIEGVMMRGQKAFAVAMRAPDGKIVTHTEKLA
                     EVYRSGLTKVPFLRGVILLWDALGLGMRALTLSANTQTGEDEKLEGPALYLTLGLSLT
                     MGIGLFFLLPAGIGGWVEHAMAQTTSALWIGNLIEGVLRLLILIGYIWSIGFMPDIRR
                     VFQYHGAEHKTINAFEAGAELTPEVVAKYPIEHPRCGTAFLLTLVLLSILVFTLLGPM
                     PIVWRLVTRVLFIPILAGIAVEYIRWTANHLNNPIVQWLIKPNLALQLLTTRPPDLEM
                     LEVAIASFQAMRKAEAETA"
     gene            complement(1048..2418)
                     /locus_tag="PKI33_08300"
     CDS             complement(1048..2418)
                     /locus_tag="PKI33_08300"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNM37049.1"
                     /translation="MTDSQKAVEYVRSNSERFLNELKDLVAIPSISTLDENKSDVQRA
                     AEWVAGQLRSIGMDNVKIMPTALHPVVYGEWMGAGKSAPTVMIYGHYDVQPVDPLELW
                     TSDPFKAEERGDYLFARGASDMKGQVVASIKAVEAIMKTEGKMPVNIKWLVEGEEEIG
                     SEHLGDFIKNNKELLAADFCLNPDAGMIGPDKPTITTGLRGLAYFELRVFGPDKDLHS
                     GLFGGTVHNPAQALIELIAGMHDKNGKITLPGFYDKVRKLSKKEREDFKRLPTTNKDL
                     IKMTGVPALWGEPQFIPAERVGARPTLEVNGLLSGFTGQGSKTVLPAWAMAKISCRLV
                     PDQTPEQTEKQLRAYLKKHAPKTIKWELKNLHNAGSALTEVDSAGVKALAKALETVWG
                     KRPYFKREGGSIGSVVLLQKYVGADSLLTGFGLPDDNVHSPNERLHLPTWYKGIEAFT
                     HFFYNF"
     gene            complement(2481..>2871)
                     /locus_tag="PKI33_08305"
     CDS             complement(2481..>2871)
                     /locus_tag="PKI33_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37050.1"
                     /translation="AALADLNTTRTPDPDPYLFWHQAEATGGQNYSQWDDRTASEFLE
                     TARTTADFNERIRLYNNFQVVFAKELPSLPLYYPVYSYGVDSQVQGVQVAPMYDVSDR
                     LSLINEWYLVTRRTLEEQSTAEPAATP"
BASE COUNT          719 a          726 c          753 g          673 t
ORIGIN      
        1 ccaagatgaa gaaaaaataa aaagagacgg gcgaaaaccc gtctctttct ctacgataga
       61 tacactttac gccgtttcgg cttctgcctt gcgcatggct tggaaggaag caatcgccac
      121 ttcgagcatt tccaaatctg gcggacgggt ggtgagcaat tgcagtgcca aattcggctt
      181 gattagccat tgcacaatgg ggttattcaa gtgattcgcc gtccagcgga tgtattcgac
      241 ggcaatgccc gccaaaatag ggatgaacaa aacgcgggtg accaaacgcc aaacgatggg
      301 cataggacct aataaggtga acacgagaat ggagagcaag accaaagtca acaaaaaggc
      361 ggtgccacag cgcggatgct caataggata tttcgccacc acttccggag tcaactcagc
      421 accggcttca aaggcgttga tggttttgtg ttcagcgcca tgatattgaa agacacggcg
      481 aatatcgggc atgaaaccaa tgctccagat atacccgatc aagatcaaca aacgtaacac
      541 gccttcgatc aagttcccga tccaaagcgc ggaggtggtt tgcgccatgg catgctccac
      601 ccagccgccg attccggcgg gaagcaaaaa gaagagtcca atgcccatgg tgagagacaa
      661 gcccaaggtc agataaagag ccggtccttc cagcttttca tcctcgccgg tttgggtgtt
      721 ggcagaaaga gtcaaagcac gcataccgag tccgagtgca tcccacaaca aaatcacacc
      781 gcgcaaaaaa gggactttgg taagaccaga gcgataaact tctgccagct tttcagtgtg
      841 tgtgacgatc ttgccatcgg gcgcgcgcat ggcaacggcg aaagcttttt gcccgcgcat
      901 catcacccct tcgatcacgg cttgaccgcc gtatgtaata attcgatctt ccataattct
      961 cgtttctttc tttcgatata caagagcgac ccgcccatgt ggatcaaagt gattgatctc
     1021 gccagacggg tcgcccttgc ttgttattta gaagttgtaa aagaaatggg taaaggcttc
     1081 aatgccctta taccaagtgg gtaaatgcaa acgctcattc ggcgagtgga cgttgtcatc
     1141 gggcaagccg aaaccggtga ggagcgaatc ggcgccaacg tatttttgca gtaagacaac
     1201 cgagccaata gaaccgcctt cgcgcttgaa gtaaggtcgt ttgccccaca cggtttcgag
     1261 cgccttggca agcgccttca caccggcaga gtcaacttcg gttaatgcgg agccagcatt
     1321 gtgcagattc ttcagttccc atttgatggt cttgggcgca tgtttcttga gataggcgcg
     1381 caattgtttc tcggtttgtt caggcgtttg gtcgggcacg agacggcagg aaattttcgc
     1441 cattgcccag gcaggcaaaa cggtcttaga accctgaccg gtaaaaccag agagcaagcc
     1501 attcacttca agagtagggc gagcgccaac gcgttcggca gggatgaatt gaggttctcc
     1561 ccacagggca ggcacgccgg tcattttgat gaggtctttg ttggtggtgg gcaggcgttt
     1621 gaaatcttcg cgttctttct tgctcaactt gcggaccttg tcgtaaaagc ccggcaacgt
     1681 gattttgcca ttcttatcgt gcataccggc aatgagttcg atcaacgctt gagccgggtt
     1741 gtggacggtg ccgccgaaca agccggagtg caaatcttta tcgggaccaa agacgcgtaa
     1801 ttcaaaataa gcgagaccgc gcaaaccggt tgtgatggtg ggcttatcgg gtccgatcat
     1861 gccggcatcg gggttcaaac aaaaatcagc ggcaagcagt tctttattgt tcttgatgaa
     1921 atcgccaaga tgttcggagc caatttcttc ttcgccttcc accaaccatt tgatgttcac
     1981 cggcattttg ccttcggttt tcatgatggc ttccaccgct ttgatggatg ccacaacttg
     2041 acccttcata tccgaagcgc cacgtgcgaa taaataatcg cctcgctctt ccgccttgaa
     2101 cgggtcagac gtccaaagct ctagcgggtc gacgggttgg acgtcataat gtccatagat
     2161 catcacagtg ggagccgatt tgcccgcccc catccattcg ccatagacga cgggatgcaa
     2221 agcggtcggc atgatcttca cgttatccat gccgatggaa cgcaattgac ccgccaccca
     2281 ctcggcggcg cgctgtacat ctgatttgtt ctcatccaaa gtagagatcg aaggaatggc
     2341 gaccaaatcc ttcaactcgt tcaaaaatct ttcactattc gagcggacgt attcaacagc
     2401 tttctgagaa tcagtcatga tgaaactcct tatagtatga atgaccctaa aggctttgaa
     2461 tgcctttagg gtctgtttgg ttacggtgtt gctgcaggtt cagccgtcga ttgctcttcg
     2521 agcgtacgac gagtgaccag ataccattca ttgatcagag acagcctgtc gctgacatca
     2581 tacatcggcg caacttgtac gccctgcact tgagaatcca cgccgtagga atagacgggg
     2641 taatacaagg gcaaagacgg caactccttg gcgaagacca cctggaagtt attatacaag
     2701 cggatgcgct cattgaagtc agcggtggtg cgggcagttt cgaggaattc gcttgcggtg
     2761 cggtcatccc attgcgaata gttctgcccg cctgtagctt cggcttgatg ccagaaaaga
     2821 taagggtctg ggtcgggggt acgagtggtg ttcaagtctg ctagagcggc t
//
LOCUS       DAOREF010000359         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23782_length_2870_cov_3.571581, whole genome shotgun sequence.
ACCESSION   DAOREF010000359 DAOREF010000000
VERSION     DAOREF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23782_length_2870_cov_3.571581"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKI33_08310"
     CDS             complement(<1..197)
                     /locus_tag="PKI33_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM37051.1"
                     /translation="MKSIVVTGVSTGIGRSISELLIQNGFHVFGSVRKQADAERLKAD
                     LGAGFTPLTFDITNEVAVQAAA"
     gene            complement(278..646)
                     /locus_tag="PKI33_08315"
     CDS             complement(278..646)
                     /locus_tag="PKI33_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37052.1"
                     /translation="MKFYYWHYGIIFCSLATAILHIMCYPYFGWLDSIVLNGFGSIAL
                     LLAYFLPIPFFRRNRQTTYWIIVSYTLLTIVLWIFLGDKAFNFENTSSIGYFAKIAEM
                     FLLIFMGFDFPRKPAPARAS"
     gene            742..897
                     /locus_tag="PKI33_08320"
     CDS             742..897
                     /locus_tag="PKI33_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37053.1"
                     /translation="MAKNISLNDIHIRTDLQPGDTGGIMSMHGRLYYKEYGYTTIFES
                     YVAKGLG"
     gene            931..1251
                     /locus_tag="PKI33_08325"
     CDS             931..1251
                     /locus_tag="PKI33_08325"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM37054.1"
                     /translation="MWVCEHAGNMVGTLVLMDRGEVAQLRYFLLEPQYRGFGLGTRLM
                     DLFMEFFQDCGYASSYLWTTEQQLTAAKLYKRYGYQLTEEKPSTAFGVSLTEQRYDLV
                     FARR"
     gene            complement(1255..1680)
                     /locus_tag="PKI33_08330"
     CDS             complement(1255..1680)
                     /locus_tag="PKI33_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM37055.1"
                     /translation="MKQQLHLITLGVKDFKRSYEFYTKTLGWKPSGASTEDVAFFQTS
                     GVVFAIYSREKLAEDALTSPDGSGFSGFTLAYNASSETEVDEIIADLKAKGVKIVKEP
                     QKVFWGGYSSYFADPDDYRWEVAYNPFFQFDEKGNLNLE"
     gene            complement(1720..2211)
                     /locus_tag="PKI33_08335"
     CDS             complement(1720..2211)
                     /locus_tag="PKI33_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNM37056.1"
                     /translation="MANHTFVKFCPRCGAPVDYKEKFGAVRPVCPQCHFIHFQDPKVA
                     AAVLIQEGSRVLLVRRVNEPHRGLWTLPAGFVNGGEDPAEAAARECLEETGLTVQVTR
                     VHDVIAGKEHERGADFIIIYAAKVISGEMNAADDADAVEWFDQDHLPPLAFAATKKVL
                     LSI"
     gene            complement(2214..>2870)
                     /locus_tag="PKI33_08340"
     CDS             complement(2214..>2870)
                     /locus_tag="PKI33_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37057.1"
                     /translation="DASNAAQIEVRLKTDAELASALRDLRAARGILKKLPARKAPRNF
                     TLTRQMVGLKPPLPRTYPLLRFATAFAAILFLFSFTATTLVPMINFGSAAAPAYGLGS
                     GGGCDACGGGGPESAPMAAAATEAPATELFAAQEASPASTLTTDAADTAPIAETETVR
                     IMETPSAKEPAPETANQAEVQQEPPLSPVNWTWIFLGIAILGGLVLWIMQFTARNKWR
                     "
BASE COUNT          757 a          661 c          690 g          762 t
ORIGIN      
        1 gctgcagctt gaacggcgac ttcattcgtg atatcgaagg tcaatggggt gaacccagct
       61 ccgaggtccg ctttgaggcg ctctgcatcc gcctgcttgc gtacactgcc gaagacatga
      121 aatccgttct ggattaataa ctcactgatc gaccgtccga ttcctgtcga tacccctgta
      181 acaacaatac ttttcatcaa gagatccttt tgaacgtgga tatactgata ttttacctat
      241 aaaataaaag agacagcttt gtgctgtctc ttttatttta tgatgctctg gcgggtgctg
      301 gctttcttgg aaagtcgaag cccatgaaga ttagcaagaa catttcggct attttggcaa
      361 aatagccgat ggaggaagtg ttctcaaagt tgaatgcttt atcgccgagg aagatccaaa
      421 gcacgatggt aagcagggta tagctgacga taatccaata ggtggtttga cggttacgcc
      481 tgaaaaatgg gatgggtaga aaatatgcca gcaataaagc gattgagcca aaaccattta
      541 gcacgatcga gtctagccag ccgaagtagg ggtaacacat aatgtgcagg atggcagtag
      601 caagactgca gaagatgata ccgtaatgcc agtaataaaa tttcattgat aatgctccac
      661 gtataaagtt ttttagtaga cgtattttat gggagattta ttacaaaggc aagcaaatat
      721 tcaatgatat gattgcgcct catggcgaaa aatatttcac tcaatgacat ccacatccga
      781 accgatttgc agcctggcga cacaggtgga attatgtcga tgcatggtcg gttgtactac
      841 aaagaatatg gatacaccac tatttttgaa agctatgtag caaaagggct gggctagttc
      901 ttagaaaaat ataaccctaa acgaaaccgc atttgggttt gtgaacatgc tggaaatatg
      961 gttggaactt tagtcttaat ggatcgtggc gaagtagcac agttgcgcta tttcttgctg
     1021 gaaccacagt atcgcggatt tggtttaggt acgcgcttaa tggatctatt tatggagttt
     1081 tttcaagatt gtgggtatgc ctcgtcctat ctgtggacga cagaacagca gttgacggct
     1141 gccaaacttt ataaacgcta tggctatcaa ctgacagagg aaaagccttc aacggctttc
     1201 ggtgtttcac ttactgaaca acggtatgac cttgtctttg ctcgtagata aaacttattc
     1261 caaattcaaa ttgccttttt cgtcaaattg aaaaaatgga ttgtacgcta cctcccagcg
     1321 atagtcatct gggtctgcaa agtaagaact atacccaccc caaaatactt tttgcggttc
     1381 ttttacgatc tttacccctt tggctttcag gtctgcaata atctcgtcca cttcagtctc
     1441 actactggcg ttgtatgcca aagtgaaacc tgagaagccg cttccatcgg gagaggtcaa
     1501 cgcatcttct gccaatttct cgcgcgagta aattgcaaac accacaccac tggtttgaaa
     1561 gaaagctaca tcttctgtgc ttgcaccaga aggtttccac ccaagtgtct ttgtataaaa
     1621 ctcatatgat cttttaaagt cttttacgcc caacgtgatc aaatgtaatt gttgtttcat
     1681 gtgtcgctcc ttttgaacaa actacatctc caccaaacgt tagatagaaa gcaatacttt
     1741 tttcgttgca gcaaatgcca gaggcggtaa atggtcttgg tcaaaccatt ccacggcatc
     1801 ggcatcatct gccgcattca tttctccact tatcactttt gctgcataga tgatgataaa
     1861 gtctgcgccg cgctcgtgtt ccttcccggc aataacatca tgtacgcgcg tcacttgcac
     1921 ggtgagcccg gtctcttcca aacattctct ggcagccgcc tctgccgggt cttcaccgcc
     1981 gttaacgaaa ccagccggta aggtccacag tccgcgatgt ggctcgttca cacggcgcac
     2041 aagcaaaaca cggctgccct cttgtatgag gacagccgct gctactttcg ggtcttgaaa
     2101 atggatgaag tgacattgcg ggcaaaccgg tcgtacggcg ccaaactttt ctttatagtc
     2161 tacaggcgct ccgcaacgtg ggcaaaactt tacaaacgta tggtttgcca tccctaacgc
     2221 cacttgttac gtgctgtaaa ttgcatgatc cacaaaacca atccgccaag gattgcgatg
     2281 ccaaggaaaa tccaagtcca gttgacggga gagagaggcg gctcttgctg gacctctgcc
     2341 tgattcgcag tttcaggagc tggttccttc gctgaaggcg tttccataat gcgtacggtt
     2401 tcggtttcag cgataggtgc cgtatcagct gcatcggtgg tgagagttga ggcaggggaa
     2461 gcttcttggg ctgcaaataa ctcagttgct ggggcttctg tggctgccgc agccattggc
     2521 gcagattcgg gcccgccgcc accacacgca tcacaaccac cgccagagcc taatccatat
     2581 gcgggtgctg ctgcgctgcc gaagttaatc atcggaacaa gcgtggtggc tgtaaacgag
     2641 aaaaggaaga ggatggctgc aaacgcagtt gcaaatctga gcaatgggta ggtacgcggc
     2701 aagggcggtt tgagccccac catttgacgg gtaagagtaa agttgcgtgg tgctttacga
     2761 gctggcagtt ttttgagaat gccacgagca gcgcgaaggt ctcttaaagc agaagcaagc
     2821 tcggcgtcgg ttttgaggcg gacttctatt tgagccgcat tcgaagcatc
//
LOCUS       DAOREF010000360         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23836_length_2866_cov_5.110993, whole genome shotgun sequence.
ACCESSION   DAOREF010000360 DAOREF010000000
VERSION     DAOREF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23836_length_2866_cov_5.110993"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /gene="mraW"
                     /locus_tag="PKI33_08345"
     CDS             <1..410
                     /gene="mraW"
                     /locus_tag="PKI33_08345"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNM37058.1"
                     /translation="RPLHTTGELVATIAKASPRRGDKTHPATQTFQALRIVVNDELAA
                     VEKTLPQAVASLNTGGRCAIISFHSLEDRIVKEYFREQSKDLINPPYERIYEVERKAV
                     VKLVNKKPILPTEEETKDNPRARSAKLRVVEKL"
     gene            451..930
                     /locus_tag="PKI33_08350"
     CDS             451..930
                     /locus_tag="PKI33_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37059.1"
                     /translation="MNIPNVNLPNVTHVIHAYRVAPWRVQRQWIGNALLVVVSLAMVA
                     ALYLNVTSRTAIAGREIQDLTDSIAASQQVSSDLQTQLANLTSAEVMQQRAKEMGFRP
                     VEPVELEYLVVPGYVAKDAVNLSSSAQLQLNVLAIPPEYNQSLLDWMDERLVARRYQ"
     gene            927..2675
                     /locus_tag="PKI33_08355"
     CDS             927..2675
                     /locus_tag="PKI33_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNM37060.1"
                     /translation="MRQQYGRRSQVLAAALAFFAFAIVFQMTRIQNSPEADTFRQLSK
                     DYSYEWRTFYPNRGEIYDRNGRLLAGEKIVYEIGVDLNIMTDAHAIAFAVADALGMNY
                     DNLMSVIQNPPDGLSYIVLADYVESSQANYLQDLKKALNEQAPEGSMGGLTGLQFKSH
                     PQRSYPENSLASNILGFVNREGKGYFGVEEKYDSLLAGNPVTVLVPTDPNKAFEIPNA
                     PDGTTLVLTINRDLQSAAEEILDNALGTYGAQDGIIAIMDPQSGEMLAVATSRRMDLN
                     EFWNYGTIYNNATEFNPIISKTYEPGSVMKILTMAAALDSNTVTPETTYLDTGEILIG
                     GVTIQNWDRNPWGVQNMVGCLQHSLNICMVTISSQMGANTFYKYMDAFGIGHLTGVDL
                     AWEAPGRLKVPGDADWYPVDLGTNSFGQGVAVTPIQMMSAVSAVANNGRMVIPHVLYA
                     MVRDGRQYEVPDQFGPTPISEQTSRTLSEMLAVSLESESSAALIPGYRVAGKTGTAQI
                     PVDGFYDSTQTNTSFIGWGPVDDPKFMIYVWLERPTASPWGSETAAPVFAEMAQRTVV
                     LLDIPPDSIRLQVAQK"
     gene            2677..>2866
                     /locus_tag="PKI33_08360"
     CDS             2677..>2866
                     /locus_tag="PKI33_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNM37061.1"
                     /translation="MLTLADVLEALTTIRPANASTIITEGAIDSRQVIPGSLFVAIPG
                     EKTDGHDYLTDAFKRGASF"
BASE COUNT          724 a          707 c          685 g          750 t
ORIGIN      
        1 cccgaccgct tcatacgacg ggtgagttgg tcgccaccat tgcaaaagca tctccccgcc
       61 gaggcgacaa gacccacccc gcgacgcaga cctttcaagc tttgcggatc gtcgtcaacg
      121 acgaattagc cgcagtggag aaaacgcttc cgcaagccgt ggcaagcctg aacaccggag
      181 gtcgatgcgc gatcatctcc ttccactcgt tggaagaccg catcgtcaaa gagtattttc
      241 gagagcagag caaagatctc atcaatccgc cttacgaacg aatttatgaa gtggagcgta
      301 aggctgttgt gaagttagtg aataagaagc cgatcctgcc gaccgaggaa gagacgaaag
      361 acaaccctcg tgcgcgcagt gccaaactta gagttgttga aaagctgtag gggcgaggtc
      421 ttcctcgcca gcaattgaga gagaactaaa gtgaacatac cgaacgtcaa cctccccaat
      481 gttactcacg tgatccatgc ctaccgggtc gctccttggc gcgtgcaacg ccaatggatc
      541 ggcaatgcgt tattggttgt ggtgagcctt gccatggttg ccgctcttta tttaaatgta
      601 acgtctcgta ctgctattgc tggtcgtgaa attcaagacc tgaccgattc tattgctgcc
      661 agccaacagg tgagtagcga tcttcagacc caactggcaa acctgacttc tgctgaagtc
      721 atgcaacaac gtgcaaaaga aatgggtttc cgacctgtgg aacctgttga actggaatat
      781 cttgtggtgc cgggctatgt tgctaaagac gcagtcaatc tttcctcctc tgctcaactt
      841 caattgaatg tgcttgccat tccgcctgaa tacaatcagt cacttttgga ttggatggat
      901 gaacgcttgg tggcacggag atatcaatga gacagcaata cgggcgccgc agtcaagtct
      961 tagctgccgc attggctttc tttgcgtttg ccatcgtgtt tcagatgacg cgcattcaaa
     1021 acagccccga agccgatacc tttcgccagc tttctaaaga ttattcatac gaatggcgca
     1081 cgttttaccc caaccgcggc gagatctatg accgtaacgg tcgcttgttg gctggggaga
     1141 agatcgttta tgaaattggc gtagacttaa acattatgac ggatgctcac gccattgcat
     1201 ttgcagtagc agatgctctt ggtatgaatt acgacaattt gatgtcagtc attcaaaacc
     1261 caccagatgg gctctcatac attgtccttg cagattatgt tgagtcttca caagcgaatt
     1321 atttgcaaga cctcaaaaaa gcattgaacg aacaagcccc tgaaggttca atgggcgggt
     1381 tgacgggctt gcagtttaaa tctcaccctc aacgtagtta tcccgaaaac tcactggctt
     1441 caaatatcct tgggtttgtt aatcgtgaag gcaagggata cttcggtgta gaagagaaat
     1501 acgatagttt attagcgggc aatcctgtca ctgtattggt gccgactgat cccaacaaag
     1561 cctttgaaat tcccaatgcg cctgatggca ctacattggt tcttaccatc aaccgtgact
     1621 tgcagtctgc cgcagaagaa attcttgata atgctttagg aacttacggt gcacaagatg
     1681 gcattatcgc cattatggat ccacaaagcg gcgagatgct tgccgttgct acatcgcgcc
     1741 gtatggattt gaatgaattt tggaattacg gcactattta taacaatgca accgagttta
     1801 acccgatcat ttccaaaacc tatgaacccg gttccgtgat gaagatcctt acgatggctg
     1861 ccgctttaga cagcaatact gtaacccctg aaacgactta tctcgacact ggtgagattc
     1921 tgatcggcgg tgtgaccatt caaaactggg atcgcaatcc gtggggtgtg cagaacatgg
     1981 tgggatgttt gcagcattca ctcaatattt gtatggtcac catttcttca caaatgggtg
     2041 cgaatacttt ctataaatac atggacgctt tcggaattgg tcacctcacc ggcgtggatc
     2101 tggcttggga agcaccaggc aggctcaagg tcccgggaga tgcggattgg tatcccgtgg
     2161 atcttggtac aaactcattt ggtcaaggtg tagcagtcac tcctatccaa atgatgtctg
     2221 ccgtttctgc agttgccaat aacggacgca tggtcatccc acatgttttg tatgccatgg
     2281 tgcgcgatgg tcgccaatac gaagtgccag atcagttcgg tcctactccc atctcagaac
     2341 agacctctcg tacattatct gaaatgctag cggtttctct cgaatcagaa tcatctgctg
     2401 cattgatccc tgggtatcgc gtggctggta agaccggtac agcgcaaatt ccagtcgatg
     2461 gtttttacga cagcacgcaa acaaatacct ctttcatcgg ttgggggcca gtagatgacc
     2521 cgaaattcat gatctatgtt tggctcgaac gccctacagc ttcaccttgg ggttctgaaa
     2581 ctgctgcccc tgtttttgct gaaatggcgc agcgaaccgt ggtcttgtta gatatccctc
     2641 ctgattcaat tcgcctacaa gttgctcaaa aataatatgc ttactctagc agacgttctt
     2701 gaagctctta ccactattcg ccctgcaaat gcctctacaa tcattacaga aggtgccatc
     2761 gattcgcgcc aagtgattcc gggttctctt tttgttgcca ttcccggcga aaaaacagat
     2821 ggacatgact atctgacaga tgccttcaag aggggggctt cttttg
//
LOCUS       DAOREF010000361         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23838_length_2866_cov_3.989328, whole genome shotgun sequence.
ACCESSION   DAOREF010000361 DAOREF010000000
VERSION     DAOREF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23838_length_2866_cov_3.989328"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKI33_08365"
     CDS             complement(<1..255)
                     /locus_tag="PKI33_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37062.1"
                     /translation="MKYSTQYSTSISRFYAIIRQSMQTFIKFSRTIDALTNKLGHASA
                     FCVMLTIAVGFYNVIARYLGRFLEVKLTSNLFIELQWYLFS"
     gene            277..804
                     /locus_tag="PKI33_08370"
     CDS             277..804
                     /locus_tag="PKI33_08370"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNM37063.1"
                     /translation="MAKNIVLTLTGKDRIGLVDQVTSLVVKRSGNVESSRMARLGGEF
                     AMLMLVTVSDAEFQNLDKDFQEMRGEGYQVTLMQTEDVSKKYAGWLPYEIEVTGADHE
                     GIIQQISHHLASQGINIESMDTSATPAPMSGTPLFMMKGIVLVPPKLNFNAWSDALEE
                     VGDTLNVSVKIEVVK"
     gene            complement(855..2054)
                     /locus_tag="PKI33_08375"
     CDS             complement(855..2054)
                     /locus_tag="PKI33_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37064.1"
                     /translation="MKKIFAIAWKDAVVRFASASELLFFIILPLVFTFLLAGGSPSGN
                     PTNNRVRLVVVDQANTAISQEIVAELEKSTAAYPELMSLADAEEEFSARRVSAVLVIP
                     AGLEISAIQNGTAELDFRQQPNDMDASIAQRAIQTAIRRVSSAVNAANVAVEEAKARG
                     TFESAEAEQAYFEEALKVAKDLQANSPERITVIEGSTPDKVDYDPRANTSAGQLVTWV
                     FIPLFGISALFAYERQQGTLRRLLTTPTSKAVFLLGTIFGQVVMALIQMSLLILFGMF
                     ALQLKWGQEPLALFILLTCAALAAGAIGTTMGTFIKSEGQASGLSIMAGMVMALLGGC
                     WYPLELFPAAVQNIVKILPTTWAMQGLLDLVLRGGGLTDILLESGVLLGFAVVFFSIG
                     VARFRYE"
     gene            complement(2051..>2866)
                     /locus_tag="PKI33_08380"
     CDS             complement(2051..>2866)
                     /locus_tag="PKI33_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37065.1"
                     /translation="KSIVDEFMSRVDESTVSGQTAIQQLLTSGRVPPEQAQVAGTELN
                     QRLTNSQQDEPLAIKLNATTADGEEIKFDVLAYLAPGMALMFLMFTVSYGGRSILAEK
                     SQGTLPRLLVSPTNATQILGGKVFGIFLTGVAQMLILIGGTSLMFQLKWGDTLGVVVL
                     VFAAVFGATGWGMLITALARTPAQVGSVGSATMLIFGILGGSFIQLDNMPATVQTLSR
                     ITPNSWALDGFTTLALGGTLANLSTPITALLTMGGVLFVISVLLFGKKNLVQK"
BASE COUNT          768 a          692 c          755 g          651 t
ORIGIN      
        1 agagaagaga taccattgca gctcaataaa gaggttgctg gtcagtttga cttcaagaaa
       61 tctgccgagg taacgcgcta tgacgttata gaatccgacg gcgatggtga gcatcacgca
      121 aaaagcagag gcgtgaccga gcttgttagt cagtgcatcg atggtgcggg aaaatttgat
      181 aaaggtctgc atggattggc ggatgatagc ataaaaacga gagatgcttg tgctatactg
      241 ggtactatat ttcacaagtc gaaagggagt tctgccatgg caaaaaatat tgtattaact
      301 ctcacaggca aagatcggat cgggttggtg gatcaggtga cgagtctggt tgtgaagcgg
      361 agtgggaatg tagaatccag ccgcatggcg cgtttgggtg gcgagtttgc catgttgatg
      421 ttggtcacag tatcagacgc agaatttcag aatctcgata aagattttca agaaatgcgc
      481 ggcgaaggct atcaagtcac cttgatgcag accgaagatg tttctaagaa gtatgcaggt
      541 tggctgccct atgagatcga agtgaccggt gccgaccatg aaggcatcat tcaacaaatt
      601 tcacatcatc tggcttcgca gggtatcaac attgaaagca tggataccag tgcaacgcct
      661 gcgccgatga gcggaactcc gctctttatg atgaaaggga ttgtgcttgt cccgccgaag
      721 ttgaacttca atgcctggag cgatgcattg gaagaagtag gggacacctt gaatgtcagt
      781 gtgaagattg aagttgtgaa ataaaagaaa agcaaacggc ggatgaacat catccgccgt
      841 ttttatttta agtgttactc gtatcgaaat cttgcgaccc caatagagaa gaacacaacc
      901 gcaaacccga gcaaaacacc tgattcaagt aggatatcgg tgagtccgcc gccgcgcaga
      961 acgaggtcaa gcaaaccttg cattgcccaa gtagtaggga gaatcttgac gatgttttgc
     1021 accgccgcag ggaataactc caacgggtac caacaaccac ccagcaatgc catcaccatg
     1081 cctgccataa tactcaaccc gctggcttga ccttcgcttt tgatgaacgt tcccatggtg
     1141 gtgccaatcg ctccggcggc gagcgcggca caggtgagca agataaagag cgcaagcggc
     1201 tcttgccccc atttgagctg gagagcgaac atcccaaaca gaatcaacag cgacatttgg
     1261 atgagtgcca tcactacttg accaaagatg gtgccaagca gaaaaacggc tttactggtt
     1321 ggggtagtga gcaaacgacg taatgtccct tgttggcgtt cgtaagcaaa gagcgccgag
     1381 ataccgaaca aaggaatgaa tacccaggtc actaattgcc ctgccgatgt attggcacgc
     1441 ggatcatagt ccactttatc tggtgtgctt ccttcaatga cggtgatacg ttcaggggaa
     1501 ttggcttgaa ggtctttggc aactttcaat gcctcttcaa aataggcttg ctctgcctcc
     1561 gcggactcaa acgtgccgcg cgctttcgcc tcttccacag ctacgtttgc ggcgttgaca
     1621 gcactgctca cacgtcgaat cgccgtttga atagcacgtt gcgcaatgga agcatccatg
     1681 tcgttgggtt gctggcgaaa gtcaagttcg gcagttccgt tttgaatggc agaaatttca
     1741 agtccagcag ggatcaccaa caccgccgaa acacggcgcg cactgaattc ttcttcggca
     1801 tctgccagag acatcaactc ggggtatgcc gcagtggact tttctaactc tgccacgatc
     1861 tcttgcgaga tggcggtatt ggcttgatct acaaccacca agcgcacacg attattggtt
     1921 gggttacctg aagggcttcc accggctagt aggaaggtga acaccaacgg caaaataatg
     1981 aagaacaaca attccgatgc gctggcaaaa cggacgactg catccttcca tgcaatggcg
     2041 aatatttttt tcatttttgc accaagttct ttttgccaaa cagcaaaacg gaaattacaa
     2101 ataagactcc gcccatcgtc agcagagcag tgatcggcgt ggaaagattc gcgagcgtcc
     2161 caccgagggc gagcgtggta aatccatcaa gcgcccagga attcggagtg atgcggctca
     2221 gggtttgaac ggttgcgggc atgttatcca attggatgaa acttccgcca aggatgccga
     2281 agatcaacat cgtggcagag ccaacactgc ccacttgcgc aggcgtacga gccaatgcgg
     2341 taatgagcat gccccagccg gtagcgccga aaacagccgc aaataccaat acaactacgc
     2401 ctagagtgtc accccatttc aattggaaca tgagcgacgt accgccgatc agaatcaaca
     2461 tttgtgctac gccagtgagg aagatgccaa agactttgcc gccaagaatt tgcgttgcgt
     2521 tggtggggga gaccaaaagc cgaggcaacg tgccttgtga tttttctgca aggatggaac
     2581 gtccgccgta actgaccgta aacatcagaa acatcaacgc catgccagga gcaagatagg
     2641 caagcacatc gaacttaatt tcttcaccat ctgcagtggt cgcattgagt ttgatcgcga
     2701 gcggctcgtc ttgctggctg ttcgtgaggc gttgattcaa ctctgtgcca gccacttgtg
     2761 cttgctctgg tggaacgcgc ccgctggtga gcaattgctg aatcgcagtc tgtccgctaa
     2821 cggtgctttc atccacgcgg ctcatgaact cgtccacgat cgattt
//
LOCUS       DAOREF010000362         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23851_length_2865_cov_4.950178, whole genome shotgun sequence.
ACCESSION   DAOREF010000362 DAOREF010000000
VERSION     DAOREF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23851_length_2865_cov_4.950178"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKI33_08385"
     CDS             complement(<1..532)
                     /locus_tag="PKI33_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM37066.1"
                     /translation="MKIKKAFNALSLLVILGMLLAACAPAATDAPVEEPVATDEAMAT
                     DEPAPAATEAAPATTRHGGWLDEIDFSVVSSDSAVSQLQAGAIDFYSFNLSSNTLQDI
                     KDAGLNYTQSYGGNYGISLNPAVFDDTTVLNPFSNRKIREAVNWLIDRNYINQEIYGG
                     GSLPKLLPITTQLVEYT"
     gene            914..>2865
                     /locus_tag="PKI33_08390"
     CDS             914..>2865
                     /locus_tag="PKI33_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNM37067.1"
                     /translation="MRKSWLVWILPFLFVLESCGTIQPATTPDARPNASLTDPLPARF
                     SMGPTTIPYGKNIRFEQISLEEGLSQSVVNVILQDQKGFLWIGTDDGLNRYDGYNFKI
                     FKPDTTNPFSLSDRSITDIVEDKDGNLWIATRMGGLNRYDPISGKFTQYMHSKNDATS
                     IISNQVTSLFVDKTGLWVGTNNGLDFLDFSSNQFIHYPLATGKLVPESKSISVVYKDS
                     ANRLWIGTSDAGLGLYDLKDQSFKSFKNNEYNNQSLSHNRILAIKQDLAGNIWVGTSN
                     GLNLYNEEEGDFTRFKRSKDDPNSIAGNIIYSLFTDQYGSLWVGTNVGLDRYSPQEGN
                     FIHHQYQPNVPNSLSNNEVLYIYEDDSGVLWVGTYGGGLNKYNRQQDRYAYFRNNPDD
                     PASLSSNIIFAIQPAEFGKVWVGTLDSGLNLFEPYTETFTRFVHDPNTPNSLINNSIF
                     SLYRDKAGLIWVGTGNGLDRYNRGSKSFTHYSPNESDFTRTDERFNVLAILEDTQKNF
                     WVGSSMGLYLFDRKAGTLTRHNSELGTTRVNALLEDKDGNLWVGTPEDGLRRLNTQTG
                     EISYFRYDSTEKTSLSNNSVLCIYQDTQGTIWIGTHGGGLSRFNPETETFTHFTEQEG
                     LSNNVVYGILEDESGKLWLSTNFGLAR"
BASE COUNT          829 a          661 c          629 g          746 t
ORIGIN      
        1 tagtgtattc aaccaactgg gttgtgatgg gcagaagttt tggtaaagaa ccgccgccgt
       61 agatctcttg attgatatag ttgcgatcga tcaaccagtt caccgcttca cggatcttgc
      121 ggttcgagaa aggattcaag actgttgtat cgtcgaacac ggcaggattc aaactgatac
      181 cgtaattacc accataagat tgggtgtagt tcaagccggc atctttaatg tcctgcaagg
      241 tattcgaaga gagattaaac gaatagaagt caattgcacc agcttgcaat tgagaaactg
      301 ccgaatcaga tgagacgact gaaaaatcaa tttcgtccaa ccagccgcca tggcgggtcg
      361 tagcaggtgc tgcttctgta gcagcaggcg caggctcatc tgttgccatc gcttcatctg
      421 tagcaaccgg ctcttctact ggagcatcgg tagcagctgg tgcacaagca gccagcaaca
      481 taccgaggat gaccaatagg ctgagcgcat taaatgcttt ttttattttc attctctttt
      541 ctccttaatg aaattgaaaa actaaccaga cagtgtacga gatagaccag gtagcacctc
      601 ctaatgaatt cgtctaagca gaaggcggaa gatcagtctg ctcactgata ctccgccttc
      661 tgagggaatt tgattcttct tggaggagaa caattccaca tgcgccttat catacgaatt
      721 ccaagggcaa ctgtcatgca tccagacaat tggttttcct cccacaattg aatggttcta
      781 aagccatcca ctgaaaaatg tttcacaacc catctcacag cgaaaaacca ttctccggta
      841 gggtgaaaca gacctccccc agagggagcc gcctttcaat cccagctcat aaattgctta
      901 taatagtttc actatgcgta aatcgtggct ggtatggata ctccccttcc tttttgtttt
      961 ggaatcttgt gggacgattc agcctgccac cacgccggat gcaagaccaa acgcctctct
     1021 aacagacccg ttacctgctc gcttctctat gggtcctacc accatcccat acggaaagaa
     1081 catccgcttt gaacagatta gtcttgaaga gggtctttcg caaagtgttg taaatgtaat
     1141 cttgcaagac caaaaaggtt ttttatggat cggcaccgat gacgggttga accgttatga
     1201 tggctacaac ttcaagatat tcaaacctga cactaccaat ccattcagtt taagtgaccg
     1261 ttcgatcact gatattgttg aagacaaaga tggaaatttg tggatcgcca ctcgtatggg
     1321 gggattaaat cgttacgacc ccatctcggg taagttcact caatacatgc atagcaaaaa
     1381 cgatgcgacc agtattatca gcaatcaagt cacatcactt tttgtagata aaaccgggct
     1441 ttgggttggc acaaacaatg ggctcgattt tcttgatttc tcatccaacc aattcattca
     1501 ctatccgctc gctacgggca agctcgtccc tgaaagcaaa tccatttctg ttgtatataa
     1561 agactctgcc aacaggcttt ggattggcac atcagacgct ggcttaggac tttatgattt
     1621 gaaggaccag tctttcaagt cgtttaaaaa taatgagtac aacaatcaga gcttgagtca
     1681 caatcgcatt cttgcgatca aacaagacct cgctggcaat atctgggttg gcactagcaa
     1741 tggattgaat ctctacaacg aagaagaagg tgatttcact cgttttaaga gatcgaaaga
     1801 tgacccaaat agtattgcag gcaatatcat ttactcctta tttactgacc aatacggaag
     1861 tttatgggtt gggacaaatg tagggttaga ccgttattct ccgcaagaag gtaactttat
     1921 tcaccaccaa tatcagccaa acgtgccaaa cagtttgagc aataacgaag tgctatatat
     1981 ctacgaagat gatagtggag tgttatgggt tggcacctac ggcggcggat taaacaaata
     2041 caaccgccaa caagatcggt atgcttactt ccgcaataat cccgatgacc ctgccagctt
     2101 aagcagcaac atcatttttg caattcaacc cgcagagttt gggaaggttt gggtgggcac
     2161 tctcgatagc gggttaaatc tatttgaacc gtacacagag acctttacac gttttgtcca
     2221 cgacccgaac acacccaaca gccttatcaa caacagcatc ttctctctct atagagacaa
     2281 agccggcttg atctgggttg gcacagggaa tgggctagac cgctacaatc gcggctcgaa
     2341 aagctttacg cactactctc ccaacgagag tgatttcaca agaaccgatg aacggtttaa
     2401 tgttttagcc attctggaag acacgcagaa aaacttttgg gtggggtcca gcatggggct
     2461 ctatcttttc gataggaaag ctggaactct taccaggcac aatagcgagc ttggcaccac
     2521 acgagtcaat gcgcttttag aagataaaga tggcaatcta tgggtaggca caccagaaga
     2581 tgggttacgc cgtcttaata cccaaaccgg tgaaatatct tatttccgat atgactcaac
     2641 agaaaagact tcgttgagca acaatagcgt gctatgtatc taccaagata cacaaggcac
     2701 tatttggatt gggacacatg gcggcggttt gagccgtttt aacccagaga cagagacctt
     2761 cacccatttt actgaacagg aaggtctatc gaacaacgtt gtatatggaa ttttggaaga
     2821 tgaatccgga aagctttggt tgagcacaaa ttttgggctt gcccg
//
LOCUS       DAOREF010000363         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23889_length_2862_cov_5.580691, whole genome shotgun sequence.
ACCESSION   DAOREF010000363 DAOREF010000000
VERSION     DAOREF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23889_length_2862_cov_5.580691"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="PKI33_08395"
     CDS             <1..1668
                     /locus_tag="PKI33_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37068.1"
                     /translation="SIWNGSMQTLNKRLLLAWGLGILPAYIAIGFLDSYYKTGVEILF
                     VSLVLQSLIAIFIYLVLGKLMLKFQQNRADAIFSAVIALAVLSFFIGLLNMASRFPNE
                     FDILSYVLPSTTLLFFCVGILAALPASIGLSALARIHNVEQTRLYKFVDANLGGIVIA
                     LLFFAIYFMLATIFNQPSFDFDDIYFDTDSALYRYRFGTEFYKDYYDRTVHPYVLIIV
                     RPFVWLVSLLLKGDMLYGAFIVTALTGALCVFLVWYFVREYTSNSLYALLIAGLFGAS
                     TSQLAFGSLIENYIFLGAAALIFMVLLLKDQPMYLLIIAGLTSFGITISNIAQTVIAH
                     FFVKRNIPQLIKYGLIVGAFIIPLNLLNNFIYPDAHPYLWEVSTLQFEEKNVFQPTIL
                     RANYLSRVMVMHSFVAPDPLFIKEDIPFIKLWLFRAAQKQDLLEIARYETALSKWLVT
                     VWLLFVAVGGVLFLKNLFKQDNRFPFAFIFIVLFSFVLHMRYGRDVFLYSTNWTYAIT
                     LFLALAWREIANKRWFQIPLFAFIAFQMINNSRLIFFMLISAYLHLR"
     gene            1683..>2862
                     /locus_tag="PKI33_08400"
     CDS             1683..>2862
                     /locus_tag="PKI33_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37069.1"
                     /translation="MQSFDKRLPLAWLLGIPLSYFIVSYFENFYKTGFQIALFTIIVH
                     SLVNLFLYYLFGRAIKEFLVNRISAVFVTILFTGVAIFIPALYNMAKPYQNLFDVSAF
                     HLDASLRISFFNALIPAFFIAMVVLHVAKQRKWDETRFFKFIDENLEGLLIAVLFFSA
                     YLIFASIFNRPSFNEDDIFFDADSRLYRWRYATENYRDYYWRPAHPFILIIVRPLVGI
                     LAFLFKGDTLFAAFTLNSLTAALCVFLVWYFVKHSAGYRLYALLIATLFGASATQLVF
                     GSIIESYIYLSAVALIFLVLLLKDSPLYLQVIAGLVAFGITISNVGQTFIAHFFIKRN
                     IKQIIIYGVMIGLFVVPLTLLNNYIYPDSQPYFWDLSLLDGEGHNQFPATLQRTNYLA
                     R"
BASE COUNT          628 a          646 c          549 g         1039 t
ORIGIN      
        1 tctatctgga atggatcaat gcagactttg aataaacgac ttctactagc ctggggattg
       61 ggcattttgc ccgcatatat tgcaataggg tttctggatt cgtattacaa aaccggggtg
      121 gaaattttat ttgtttcgct tgttcttcaa tctttaatcg ctatatttat ctatttggtg
      181 cttggaaagt tgatgttgaa attccaacaa aaccgagccg atgcaatctt ttctgccgtc
      241 atagctcttg ctgtgctctc cttttttatt ggattgctca acatggcaag tcgcttcccc
      301 aatgaatttg atattctgtc ctatgttttg ccatcaacaa cattgttgtt cttttgtgtt
      361 gggattttag ctgctttacc tgcttcaatc ggtttatcag ctcttgcaag aatacataat
      421 gttgagcaaa ctcgccttta taaatttgta gacgcaaacc tgggtggaat tgtgattgcc
      481 ttgctgttct ttgcgattta tttcatgctt gcaactattt tcaaccaacc cagctttgac
      541 tttgacgata tttactttga tactgatagt gcactctatc ggtatcgttt tggtacagaa
      601 ttttataaag attattatga ccgcactgtt cacccctatg tgctaatcat cgttcgacca
      661 ttcgtctggt tggtatccct ccttctcaag ggcgatatgc tttatggcgc attcatcgtt
      721 actgcattga ctggcgctct gtgtgtcttt ctcgtttggt attttgtaag ggaatacacc
      781 tcaaactcac tgtacgctct gttgattgca ggtttgttcg gcgcttctac atctcaactc
      841 gcgtttggtt ctctaatcga gaactacatc ttcctcggag cggctgctct gatcttcatg
      901 gtcttgcttt tgaaagacca gccgatgtat ttgctcataa tcgccgggtt gacctctttt
      961 ggcattacta tctccaatat cgcacagacg gtgattgcgc atttctttgt caagcgtaat
     1021 attccgcaac tcataaagta tgggctgatt gtaggtgctt tcatcattcc attgaatctg
     1081 cttaataact ttatttaccc cgatgcacat ccctacttat gggaagtgtc aactctgcag
     1141 tttgaagaaa aaaatgtatt tcaaccaaca atcctccgcg ctaattactt gagccgtgtg
     1201 atggtgatgc acagctttgt cgcgccagac ccattattta taaaagaaga tattccattt
     1261 atcaaacttt ggctattccg tgctgcgcaa aagcaagacc ttctcgaaat tgcccgatac
     1321 gaaacagcac tgagtaaatg gcttgtcacg gtttggcttt tgtttgtagc cgtaggtggt
     1381 gtccttttcc tcaaaaatct tttcaagcag gataatcgtt tcccatttgc ttttattttt
     1441 attgtcttgt tctcttttgt gcttcatatg cgttacggaa gagacgtgtt cctgtactcc
     1501 acaaactgga cctatgccat caccctgttc cttgccttgg cgtggcgtga aattgccaac
     1561 aagcgctggt tccagattcc gcttttcgcc ttcattgctt ttcaaatgat caacaattca
     1621 cgtttgattt tctttatgct gatttcagca tatctccacc tccgctaatc aaggattctc
     1681 atatgcagtc attcgataaa cgcctccctt tggcttggtt gctagggatt ccactttcgt
     1741 atttcattgt ttcgtatttt gagaatttct ataaaactgg ctttcagatt gctctattca
     1801 ctatcatcgt tcacagtctg gtcaatttgt ttttgtatta tctttttggt agagcaatca
     1861 aagaatttct tgtaaaccgc atcagtgcgg tttttgtaac tattctattt actggtgtgg
     1921 cgatttttat tccagcttta tataacatgg caaagccgta ccagaatttg tttgatgtaa
     1981 gtgcttttca tctggatgca agccttcgta tctcgttttt caatgctttg attcctgctt
     2041 tttttatcgc gatggtcgtt ttgcatgttg ccaagcaaag aaaatgggac gagacgcgct
     2101 tcttcaaatt catagacgaa aatttagaag gcttgctgat tgccgttctt ttcttttctg
     2161 cttacctcat cttcgcatct atcttcaatc gtccatcctt caacgaggat gatattttct
     2221 ttgacgcaga tagccgtttg tatcgttggc gttatgccac agaaaactat cgcgactatt
     2281 attggcgtcc tgctcatcca ttcatcctta ttatcgttcg cccgttggtt gggattcttg
     2341 ctttcctttt caaaggggac accctcttcg cagcttttac cctcaattcg ttgaccgccg
     2401 ccttgtgcgt tttcttggtc tggtattttg ttaaacactc tgctggatat cgtctctacg
     2461 cacttttgat cgcaaccctt tttggcgcct ctgcgactca actcgtgttt ggttctatta
     2521 tcgagagcta tatctacctt tcagctgttg cgttgatctt ccttgtcctg ttgctaaagg
     2581 atagcccttt atatctacaa gtcattgctg ggttggtagc gttcggtatc actatctcta
     2641 acgtcgggca gacgttcatt gcgcacttct tcatcaaacg caatataaaa cagatcatta
     2701 tttatggagt gatgattggc ttgtttgtcg ttcctctcac cttgttgaat aactatatct
     2761 atcctgactc tcaaccgtac ttttgggatt tatccctgct cgacggtgaa ggacataatc
     2821 aatttcccgc cacccttcag cggacaaact atcttgcacg tg
//
LOCUS       DAOREF010000364         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23902_length_2862_cov_2.873174, whole genome shotgun sequence.
ACCESSION   DAOREF010000364 DAOREF010000000
VERSION     DAOREF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23902_length_2862_cov_2.873174"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="PKI33_08405"
     CDS             <1..1494
                     /locus_tag="PKI33_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNM37070.1"
                     /translation="ISDFIFAAIAEETGLLGTLGLIATIWLILARGLTIALRAPDNFR
                     RYLAAGIVAYLGVQSLLIIGGNLRLLPLTGVTLPFVSYGGSSLLTSFIALYLLLSISS
                     VEDDEPAALSNPAPYSILAGIFALGFAACALTSSWWGLVRNADLLDRTDNARRAIADR
                     YVPRGKILDNNNQPINVSTGESGSFIRTYLYPELAPITGYTHPVYGQAGLEANLDDYL
                     RGLQGNPTSLILWNQLIYGTPPAGLDVRLSLDLNLQSTADALLGKHRGAIILMNAESG
                     EILVMASHPTYDPNKLDEEGDSLSQNAAAPLLNRATQGMYPVGDALLPLLKANFGEAT
                     VTDNELLSFYDQLGLFRSPLITLPVAESQPTEKVQDLRASPLQVALATAALSNHGVIP
                     APRIATAVNTPEQGWVVLPVLDKPRELFSAEIADEAAHAFVQQGNAYWKYIGQGVISK
                     TTVTWLIAGTPPDWQGAPLAVVIAVEENNPQLVEQIGASLFNSVLSQ"
     gene            complement(1540..1953)
                     /locus_tag="PKI33_08410"
     CDS             complement(1540..1953)
                     /locus_tag="PKI33_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNM37071.1"
                     /translation="MTHIITSLCVRDRGCIEVCPVECIVPGNPADGFPMVYIDPDTCI
                     DCGACVPECPFSAIFPEDEVPSSYTAKGTEYINRVGLSGHYEASNHHGKPVVLETTKK
                     LDAGETVDLTPDTQLNYDFFKSGPGYSAKDTDSGS"
     gene            complement(2074..2676)
                     /locus_tag="PKI33_08415"
     CDS             complement(2074..2676)
                     /locus_tag="PKI33_08415"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNM37072.1"
                     /translation="MAFELPKLAYAYDALEPHIDARTMEIHHTKHHQTYITNLNAAVE
                     KTPELQGKSLEALLSDLNAVPEGVRAVIRNHGGGTYNHNLFWEIMGPNAGGAPKGDLA
                     KAIDASFNSFDAFKDEFTKAATTRFGSGWAWLVKKGSGLAVVSTANQDTPLSDGLTPI
                     LGIDVWEHAYYLNYQNRRPDYITAFYNVINWEAVSAKFAA"
BASE COUNT          689 a          697 c          747 g          729 t
ORIGIN      
        1 atttccgatt ttatttttgc cgccatcgca gaagaaacgg gtctactcgg gaccttgggg
       61 ctgatcgcca ccatctggtt gattctcgct cgcggcttga ccattgcctt gcgggcgcca
      121 gataactttc ggcggtattt agcggctggg attgtggcat acctcggtgt gcaaagcctg
      181 ctgatcattg gcggaaattt acgtctgctt ccgcttacgg gggtgactct gcccttcgtt
      241 tcctatggcg gctcttcttt attaacttct ttcattgcct tatatttatt actgtctatc
      301 agtagcgttg aagatgatga acccgcggcg ctttcgaacc cggctccata ttccatcctt
      361 gcaggcatct tcgcccttgg cttcgccgct tgtgctctca caagctcatg gtggggactt
      421 gtacgaaatg cagacttact tgaccgcacc gataacgccc gccgcgccat tgcagaccga
      481 tacgttccgc gcggcaaaat tttagataac aataatcaac ccattaacgt ttctacgggt
      541 gaaagcggct ctttcattcg cacctatttg taccctgagc ttgcgccaat cacgggatat
      601 acccaccccg tctatgggca agctggtttg gaagccaacc ttgatgatta tctacgcggg
      661 ctacaaggca acccaaccag cctcatcctc tggaatcaac tcatttatgg cacaccgcca
      721 gccggattgg atgtgcgttt aagcctggac ctcaatttgc aatctacggc agatgctttg
      781 ttaggaaaac atcgaggcgc catcattctc atgaatgcag agagcggcga gattctggtc
      841 atggcatcgc atcctacgta tgaccccaac aaacttgatg aagagggaga ctccctttca
      901 cagaacgctg ccgcgccgtt actgaatcgc gccacccaag gtatgtaccc agttggagat
      961 gcacttctgc cgctcttaaa ggcaaacttc ggcgaagcaa ccgttaccga taacgagttg
     1021 ctctcttttt acgatcaact cggactcttc cgttccccat taattacctt acccgtggca
     1081 gaaagccagc ctactgaaaa agtgcaagac ctgcgagcca gcccattaca agttgcatta
     1141 gcaacagcag cattgagcaa ccacggagtg atccctgcgc cacggattgc aacggcagta
     1201 aacacgcccg aacagggatg ggtggtgctg ccagtgttgg ataagccgcg agagttgttc
     1261 tcagccgaaa ttgcggatga ggcggcgcat gcttttgtcc agcaagggaa tgcgtattgg
     1321 aaatatatag gtcagggtgt gatcagcaag acgacggtca catggctgat tgctggaacg
     1381 ccgcccgatt ggcaaggtgc tccgttggca gtggtaatcg cggtggaaga aaataatccg
     1441 caactcgttg aacagatcgg cgcaagttta ttcaattcgg tgttaagtca gtagaatcaa
     1501 aaaagccagg catgtgcctg gctttttatt ttcaatggac tagcttccgc tatcggtgtc
     1561 tttggcgctg tagccaggac cactcttgaa gaagtcatag ttgagctgtg tatcaggagt
     1621 gagatcgacg gtttcaccag catcgagctt cttggtggtt tcgagaacaa ccggcttgcc
     1681 gtggtgattg gaagcttcgt agtgaccaga gagaccaaca cgattgatgt actctgtgcc
     1741 cttggcagtg taagatgaag gaacttcatc ttcagggaag atggcactga aaggacattc
     1801 gggcacgcaa gcgccgcagt cgatgcaggt gtcaggatcg atatagacca tggggaagcc
     1861 atcagcagga ttgccgggga cgatacattc gacggggcaa acttcgatgc atccgcggtc
     1921 acgaacgcac aaactggtaa taatatgagt catttctttt ctccttgtgg gatatagaca
     1981 aacgatttct ttctattata actttgttta cgtgagtgac aaggacaaaa aaacaccccg
     2041 tattgctacg gggtgttttt tacatcgtac gtactaagca gcaaattttg cagagacagc
     2101 ttcccaattg attacattgt aaaacgcggt gatgtaatcg gggcggcggt tttgatagtt
     2161 caagtagtaa gcgtgttccc acacgtcgat gccgagaatg ggagtgagac cgtcagaaag
     2221 cggagtgtct tgattggctg tggagacaac ggcgagtccg ctgcccttct tgacgagcca
     2281 agcccagccg gagccgaagc gagtggtggc ggctttggtg aattcatcct tgaatgcatc
     2341 aaaggagtta aaggaagcat caatcgcctt ggcgaggtca cctttggggg cgccgccggc
     2401 gttgggtccc ataatttccc agaaaaggtt gtgattgtag gtgccgccgc catgattgcg
     2461 gataacggcg cgaacacctt caggaactgc gttcaaatcg ctgagcaagg cttcgaggga
     2521 tttgccctga agttcggggg tcttttcaac agcggcattg aggttggtga tataggtctg
     2581 atggtgcttg gtgtgatgga tctccatcgt gcgcgcatca atgtggggtt caagcgcatc
     2641 gtacgcgtat gcaagtttgg gaagttcaaa agccataata gttctccttc gagttgatta
     2701 tttgagttgg tctttccatt gtatcccgcc cgtgggctgg gcgcaagata aaataaattt
     2761 tattacaaat atctaataaa tccaaaggca aaaaaagccg ccataatttc ttacgacggc
     2821 ttttctacaa ccttacttct tatcgccacc gagtaagccg cc
//
LOCUS       DAOREF010000365         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23910_length_2861_cov_3.820741, whole genome shotgun sequence.
ACCESSION   DAOREF010000365 DAOREF010000000
VERSION     DAOREF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23910_length_2861_cov_3.820741"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKI33_08420"
     CDS             complement(<1..301)
                     /locus_tag="PKI33_08420"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNM37073.1"
                     /translation="MKNTLYLLIASVLFLAACASPTPAPTADPASSTMPALTDPSKPI
                     TVSAGETFMIVVESNPSTGYHWEIVGDMTNIEFVSREFTGSEPVIPGSGGVEVWTF"
     gene            complement(398..763)
                     /locus_tag="PKI33_08425"
     CDS             complement(398..763)
                     /locus_tag="PKI33_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37074.1"
                     /translation="MEIKHLGDAVLNDILSSNVESLSMIETVPHEIALIHNGQGYNVE
                     LGLLSVGKVHDSVYSKFPPVPKNLKFVVIILSQHATRAMMGSALEILDRVFFRRKMTA
                     VVASMQEAEAQIMQMGFSG"
     gene            complement(852..2786)
                     /locus_tag="PKI33_08430"
     CDS             complement(852..2786)
                     /locus_tag="PKI33_08430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM37075.1"
                     /translation="MFKFTVIAATLLITASLNIISTTISWSRAKSRIGLYFAIGMTGV
                     TFWTLASGLDYAAVPIPLKIYFAKWEYVFYHITLVFFLMFIMSFAGYKTLLKNKLVIT
                     VLWVIPTINILLAWTNDWTGWLWVGFVESNFGNNTIIFEHGFAFLWVTATGYMLLASI
                     IAASWIAMRRSFGFARRQGQVLFYGSLLPLIGNLIYLFQPPAFDGVDWSSIFISGSSV
                     LFLWAFYGTNLINVIPIAREKLIDRSGDGMIVLDSRNYIIDINESAAKLLNLTVASLL
                     GKKLADYLPDAKFLSREALEEELSTELEIEAPTPRFFDVLVTPLFEGQKTIIGSLVVL
                     RDITKRRQNELRLLLLNQTVEQSPNSVVITDFDGKITYVNSAFTAITGYSAKEVLGKK
                     TSILKSGYMSKEVYENLWQTILSGKTWEGELNNMKKGGELFWENTRIAPLLNQDGEIS
                     NFVAIKVDITERKRAQDELERMAITDPLTGLFNRRYFFEVAQKEFLKSIRYNRPLSVV
                     LFDIDMFKSINDTYGHLAGDQILAQIGVLLTQKERETDLSARYGGEEFVLLLPETDCE
                     GARNTAERLRSLLETSPIYYENHKIQFTASFGVAGIGNRDYPETFDYLILQADKALYE
                     AKRISRNTVVCSCETQSEIK"
BASE COUNT          849 a          592 c          584 g          836 t
ORIGIN      
        1 tgaaggtcca gacttctacg ccgccagagc cggggatgac gggttctgag ccggtgaatt
       61 cacgcgaaac gaattcaata ttcgtcatgt cgcctacgat ttcccagtga taaccggtgg
      121 acgggttgct ttcgacgacg atcatgaagg tttcaccggc gctgactgtg attggcttag
      181 atggatcggt aagggcaggc atagtggaag atgccgggtc tgcggtggga gcaggggtag
      241 ggctggcgca ggctgctaaa aatagtacgg aggcgattaa taaatataag gtgtttttca
      301 tgtaaggctc ctttggttta tagggagata acgttcaata tgaaaatttt gttcccggtt
      361 aaagagaaaa ggatgcaaat gtaaggattg tcttcttcta gcctgaaaac cccatttgca
      421 taatctgagc ttctgcctct tgcatggatg cgaccacggc tgtcatcttg cgtcggaaga
      481 agaccctatc caatatttcc aaggcggagc ccatcatggc acgggtggca tgttgactca
      541 agatgatgac tacgaatttc aggttttttg gaacgggtgg aaatttgcta tacaccgagt
      601 cgtggacctt gccaacgctg agcagaccca gctctacgtt gtagccttga ccgttgtgaa
      661 tgagcgcgat ttcatgcggc acggtttcaa tcatcgagag cgattcgaca tttgacgaaa
      721 ggatgtcatt caagaccgca tcgccgaggt gtttgatttc caaaaggcat gaatttatat
      781 tttcagcgtg cggtacttca tacgatgcgt ggtgaggtct ttgtcgaatt tttcttcatc
      841 accattgaaa ttcatttgat ttccgattgt gtttcacaag aacaaacgac tgtatttcta
      901 ctgattcgtt tggcttcata taaagcttta tcagcttgta aaattaaata atcaaaagtt
      961 tctggataat ctcgattacc aataccagct accccaaaac ttgcggtgaa ttggatttta
     1021 tgattttcat aataaatagg ggaagtttcc aacaagctac gcaatctctc tgcagtattt
     1081 ctagcgcctt cacaatctgt ttcaggtaat aataaaacga attcctctcc cccatatcga
     1141 gcagaaagat ctgtttcacg ttccttttgg gtcagcagaa ccccaatttg agccagtatc
     1201 tgatcaccag caaggtgtcc atacgtgtca ttgatacttt tgaacatatc aatatcaaat
     1261 aaaaccacag atagaggtct attatagcga atagatttta gaaattcttt ctgagcgact
     1321 tcaaaaaaat aacgacgatt gaacaacccg gtaagagggt ctgtgatagc catacgctcc
     1381 aattcatcct gagctcgttt gcgttcagta atatccactt tgatggcgac aaagttactg
     1441 atttctccat cctgattcag taatggcgcg atgcgggtat tttcccaaaa aagctcgcca
     1501 ccttttttca tattattcag ttcaccttcc caagttttcc cagacagaat cgtttgccaa
     1561 agattttcat atacttcttt tgacatatat ccagatttca gaatggatgt tttttttcca
     1621 agcacttcct ttgctgaata accagtgatg gcagtaaatg ctgaattgac atatgtgatt
     1681 ttgccatcga agtcggtgat tacaactgaa tttggacttt gttctactgt ttgattaagc
     1741 aaaagcaggc gtagttcatt ttgtctgcgt ttggtaatat ctcttaaaac gaccagagat
     1801 cctattatcg ttttctgtcc ctcaaataaa ggggtgacaa ggacatcaaa gaaacgtggg
     1861 gtgggcgctt cgatctctaa ttctgtactt aattcttctt ccagtgcttc tcttgacaag
     1921 aatttggcat caggcaaata gtcagccagt tttttaccta ataggcttgc aactgttaaa
     1981 tttagtagtt ttgctgcgga ctcgttgata tcaataatat aatttcttga atccaagaca
     2041 atcattccat cccctgaccg atcaatcaat ttttcacgag cgattggaat gacatttatt
     2101 aaatttgtgc cataaaatgc ccaaagaaat aagacacttg acccagaaat aaaaatactt
     2161 gaccaatcta cgccatcgaa tgcggggggt tgaaataagt agataaggtt tccaataagt
     2221 ggtaggaggc tgccataaaa tagaacttgt ccctgtcgac gtgcaaaccc aaaacttcta
     2281 cgcattgcaa tccacgaagc ggcaataata cttgccaata acatatagcc tgtcgcggtt
     2341 acccacaaga acgcaaatcc atgttcgaaa ataattgtat tattcccgaa gttgctttca
     2401 acaaatccaa cccataacca accagtccag tcattcgtcc atgcaagtag gatgttaata
     2461 gtaggtatca cccacaacac tgtaatgacc aatttattct tcaataatgt cttatacccc
     2521 gcaaacgaca tgataaacat caagaaaaag accagtgtaa tgtgataaaa aacgtattcc
     2581 catttagcaa aataaatttt taaggggatt gggacggctg cataatcaag cccggatgcc
     2641 aatgtccaaa aagtaacccc ggtcatccct atggcaaagt aaagtccaat gcgagatttt
     2701 gctctactcc aactaatagt tgttgaaatg atatttagac ttgctgtaat taatagggtt
     2761 gcggcaataa cggtaaattt gaacataatc tcttgcaata gtttacatga tttaaaagaa
     2821 attacaaaac ttcatatccc cttagaaatc aaaaggaacg g
//
LOCUS       DAOREF010000366         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_23929_length_2860_cov_3.091266, whole genome shotgun sequence.
ACCESSION   DAOREF010000366 DAOREF010000000
VERSION     DAOREF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23929_length_2860_cov_3.091266"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKI33_08435"
     CDS             complement(<1..839)
                     /locus_tag="PKI33_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37076.1"
                     /translation="MLLQRAKPYFIGLLLVIAIIIPFLVAGYTSLNRAENAYSTEQYR
                     LAAEEYAHTARILFWRKDLYGRAGISAAQGEDFEGAIAYFKQAKNLTEMGWVLFCQSY
                     AQLNDYPNTISTCKAGLQKYQSAPLYKILAFVYRDQKDWVAELDALKHQTQLDPNDAY
                     AAYRLGVLMALYAPEDSIPELTRASSLNPEVDSAVQTLRAALAVSSQQSDPSMQKVIV
                     GQAFGLVQDWELAKTAFEQAVQQNENNAEAWAWLGEAKQQTGQDGSAELDQALLLDHN
                     SVNV"
     gene            891..>2860
                     /locus_tag="PKI33_08440"
     CDS             891..>2860
                     /locus_tag="PKI33_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNM37077.1"
                     /translation="MSDFIQLALNQLTVPPGDMIYYIVLAFTIASALQSAFNHWRASE
                     FPQARRAFIGLGILLSAQIFMFLFSGLGWQELIDSKAVLPPMDRAFLVFGIVWISWLY
                     AFPEPNRQADAVATLLSMFIMTALGLSLISWQQQMANPATATLSYNLTLDDFIWQIGS
                     VLLALLGMAVLFIRRPDGMWYGLILLFLGFLGHVGQLLIGLEGDFSGIVRLAYITAFP
                     ILLTLPQRFGTVTVVETSAPVKPTILKQDTAGRPERRRYSTDPKTFHALLAVAAENNS
                     TKMSQAITRAIAQTMLADLCFMIYLTDNNNQMVIAGGYDLIREDTLEGGSLSKNAIPM
                     LANSIQRGRPLRLPASSTSADIKGLGDILGLTNPGHLLYVPILTPEKETLGGILLLSP
                     YSDRTWSAEDQAFLSNIASSLVPIIQRNQKITKLEAQSDMARAQVADLEERIRNLNGQ
                     LDAARTEVERNKSTDVASLRVAQEESQNIIEQLQRENAELRSGKNLPSAQAEKELKAT
                     LQEMAILQNQLADANIKLLELEKGKIATKSTEQAEVLASISQELRQPLSSIVGYTDLL
                     LGESVGILGALQRKFVDRIKVSTERIRSLTDDMIQITTLATDLYSLNPEAVDLNSIID
                     NAMSYTSSQIREKNISIHLEVPKAVTSIHADR"
BASE COUNT          726 a          726 c          655 g          753 t
ORIGIN      
        1 acattgaccg aattatggtc taggagtaat gcctgatcaa gttccgcgct tccatcttga
       61 cctgtttgct gtttcgcctc tccgagccac gcccacgcct cagcgttgtt ttcattttgt
      121 tgaaccgctt gctcaaatgc tgtttttgcc aattcccaat cttgtaccaa gccgaatgcc
      181 tgccccacga tcaccttctg catagaagga tcgctttgct ggctggaaac tgctaaagcg
      241 gcgcgcaagg tttggacagc agaatctact tcggggttaa gagaggaggc gcgtgtcagc
      301 tccgggatgg aatcttccgg ggcgtacaaa gccatcagca ccccaagtcg atacgcggca
      361 taggcgtcgt ttgggtctaa ttgagtttgg tgcttaagtg catcaagttc tgccacccaa
      421 tctttttgat ctcgatacac aaacgccaaa attttatata acggtgcaga ttgatacttc
      481 tgaagccctg ccttgcaagt tgagatcgtg tttgggtaat cgttcagttg tgcatacgat
      541 tggcaaaaca agacccaccc catttcagtg aggttcttgg cttgtttaaa atatgcaatg
      601 gcgccttcaa aatcttcgcc ttgcgccgcc gaaatacccg cacgcccata cagatccttt
      661 cgccagaaca gtatgcgggc agtatgggca tattcttcag ccgcaagccg atattgctcg
      721 gtagaatacg cattttcagc ccggtttagg ctggtatagc ctgccacaag gaacggaatg
      781 atgatggcga tgacaagcaa aagacctata aagtagggtt tggctctttg tagcagcata
      841 atttcaattc tacgccataa tccctaactg gcagtataat cacgagcgga atgagcgatt
      901 ttatacagtt agcattaaac caactcactg ttccaccggg agatatgatc tactacatcg
      961 ttctggcgtt taccatcgcc agcgcgttgc aatctgcatt taatcattgg cgggcaagtg
     1021 aatttccaca agcacggcgt gctttcattg gtctgggcat tttgctcagc gcacaaatct
     1081 ttatgtttct cttcagcggg ctgggctggc aagaattaat cgactcgaaa gccgtcttac
     1141 cacccatgga ccgtgccttt ttggttttcg gcatcgtgtg gatctcttgg ttatacgctt
     1201 tccccgaacc caatcgccaa gctgatgcag ttgccaccct tctcagcatg ttcattatga
     1261 ctgccttggg gctgagttta atctcatggc aacagcaaat ggcaaaccct gccacagcta
     1321 ccttgagcta caacttaacc ttagatgatt tcatctggca gatcggttct gtattgctcg
     1381 ctttgttggg catggctgtg ctgttcatcc gcaggcccga tgggatgtgg tacgggctga
     1441 ttttgttatt ccttggcttt ttagggcacg tcggtcagtt attgatcggg ctggaaggcg
     1501 atttctctgg aatcgtccgc ttggcgtata taacagcttt ccccatttta ttgacacttc
     1561 cacagcgttt cggcacagtg accgtggtcg aaacgtcggc tccagtcaaa ccgaccattt
     1621 tgaaacaaga tacagcgggt cgccctgaac ggaggcgtta cagcacagac ccgaaaacat
     1681 tccatgcctt gttggcagtt gccgctgaga acaactccac caagatgagt caggcaatca
     1741 cacgtgccat tgcacaaacc atgcttgccg acttgtgctt tatgatctac ctgaccgata
     1801 acaacaacca aatggtgatc gccggcgggt atgacttgat ccgcgaagac acattggaag
     1861 gcggctcgct atcgaagaat gccattccaa tgctggcgaa ctccatccaa cgcggacgtc
     1921 ctttacgttt acctgccagc agcacctcag cagatatcaa aggcttgggc gatattcttg
     1981 ggctcaccaa ccctgggcat ttgttatatg tccccatcct caccccagaa aaagaaacct
     2041 tgggcggtat tcttctctta tctccatact cagaccggac ctggagtgct gaagatcagg
     2101 ctttcctttc taatattgct tcttctttgg taccgattat tcaacgtaac caaaagatta
     2161 ccaagctcga agctcaaagc gatatggctc gcgcccaagt tgcagatttg gaagaacgca
     2221 ttcgcaattt gaatggtcaa cttgatgcag ctcgcacaga agttgagcgt aataaatcaa
     2281 cagacgtcgc ttcattacgc gtagctcagg aagaatccca aaatatcatc gagcagttac
     2341 agcgcgaaaa tgcagaatta cgctctggaa agaacctgcc ttctgctcaa gcagaaaaag
     2401 aattaaaagc aaccttgcaa gaaatggcaa ttttacagaa ccagttggca gatgctaata
     2461 tcaagctttt agaattggaa aaagggaaaa tcgctaccaa gagcaccgag caagcagaag
     2521 tgctcgcttc catttctcag gaacttcgtc agccgctttc ttctattgtc gggtatacag
     2581 acctgttact gggcgagtca gtcggtattc tcggtgcgtt acagcgcaaa tttgtggatc
     2641 gtatcaaggt ttccacagaa cgtatccgca gcctgacaga cgatatgatc caaatcacaa
     2701 cgctggctac cgacctgtat tcgctcaatc ctgaagctgt agacttgaac tcaatcatcg
     2761 acaatgccat gtcgtacact agttcacaga ttcgtgagaa aaatatttcc attcatttgg
     2821 aagttccaaa ggcagtcaca tccatccacg cggacaggga
//
LOCUS       DAOREF010000367         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24191_length_2841_cov_4.853553, whole genome shotgun sequence.
ACCESSION   DAOREF010000367 DAOREF010000000
VERSION     DAOREF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24191_length_2841_cov_4.853553"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKI33_08445"
     CDS             complement(<1..267)
                     /locus_tag="PKI33_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37078.1"
                     /translation="MNRNKITDIFIYIGLMIAFLTPRLPGIGSFVTLDEPQWLSQGAN
                     FYYALGQREFQNTVYEYQPAVTTMEIISLAMFLYFPEYRGLGQGY"
     gene            complement(334..966)
                     /locus_tag="PKI33_08450"
     CDS             complement(334..966)
                     /locus_tag="PKI33_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37079.1"
                     /translation="MKILLCDDQAVIRDGLEMLLQLEKDFQVIGSAQDGFEAVELASK
                     KSPDLVLMDLKMPGMNGIEATREIRKKHPNMKILVLTTYDDDEWVFDAIRAGASGYLL
                     KDTPRQKIIEAIRGTMDGKSFVDPAVAGKLLNQVANNQTQPASILKDKLTERELDVLR
                     LLAKGMTNTDIAGTLHLSEGTVRNHVSAILEKLGVSDRTQAAVIAIQHGL"
     gene            complement(1027..2352)
                     /locus_tag="PKI33_08455"
     CDS             complement(1027..2352)
                     /locus_tag="PKI33_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNM37080.1"
                     /translation="MHSTDDSTRILRTAAWIWLGFLLAMALMDIALYMPQVQNLLGDS
                     TRLQPALPNQPLPPTPIGNLPNSPLRGPHYPVFIFYTANGLTALFFLALTHWSDIQAK
                     MEDIYYPFLLSVISVAPIVINAFIIPRFPQGPLSNAEGMALRQLPVMFVALVLVAWNY
                     RFLHVVLFSIVTTFIELGLVAINPSDNRNITVYIFIAIIRTISFVAVGFFISFIVSRL
                     RKQRESLREANANLTHYASTLEQLTVSRERNRLARELHDTLAHSLTAISVSLETAKAY
                     FDIDLNQSREMIDTSLEATRKGVDETRRALKALRSSDLVDMGLRLAVKKAAESAASRF
                     SLDLELDLQDPMPSLSPDVEQTILRVTQEAIENMTKHSQAKKFSVRLASDKHTTLVIQ
                     DDGIGFDMKPKDSTGHFGLVGMRERAELAGGTLKIESGKGKGTKVVLTI"
     gene            2502..>2841
                     /locus_tag="PKI33_08460"
     CDS             2502..>2841
                     /locus_tag="PKI33_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNM37081.1"
                     /translation="MKTIATVPTSLQKQEVTRFARFLTVGAVGTLLDFSILTLLKLAG
                     MPTLFANSLSFTAGLLNNFTWNRIWTFSDTAKADWRKQLAQFTLVSLAGLVLNNLIVL
                     SLEGVLGNILG"
BASE COUNT          771 a          660 c          673 g          737 t
ORIGIN      
        1 gtagccttga cccaacccgc ggtactctgg aaagtataaa aacatcgcca gagagataat
       61 ttccattgta gtaactgcag gctgatattc atagacggta ttttgaaatt ccctctgtcc
      121 taatgcgtaa taaaagtttg ctccctgact caaccattgt ggttcatcaa gagtgacaaa
      181 agacccaata cccggcaaac gcggagtcag aaaggcaatc atcaacccaa tgtagatgaa
      241 aatatccgtt attttgttac gattcatgtc caagattata ccgttacatc ccccatcgaa
      301 gagagacggt caccttaatg gtgaccgtct ctcttataac ccatgctgta ttgcaatgac
      361 tgctgcttgt gtcctatcgg acacgcctaa tttttccaga atagcgctga cgtggtttcg
      421 gactgtccct tcggagagat gcagggttcc ggcaatgtcg gtgttggtca tgcccttggc
      481 gagcaggcgg agtacatcca attcgcgctc ggtgagcttg tctttcagaa tggaggcagg
      541 ttgggtttga ttgttggcaa cttggttgag caatttccct gccacagccg ggtcgacaaa
      601 agacttccca tccatcgtgc cacgaatggc ttcaatgatc ttttggcgtg gggtgtcttt
      661 gagcaaatat cccgaagccc cagcgcggat ggcatcgaag acccactcgt cgtcatcgta
      721 agtggtaagg acgaggattt tcatattcgg gtgtttctta cgaatctcac gtgtagcttc
      781 aataccgttc atccccggca tcttcaagtc cattagaaca aggtctggag atttttttga
      841 agcgagttcc actgcctcaa atccgtcttg agcagaaccg atcacttgga aatctttctc
      901 caattgaaga agcatttcca acccgtcgcg gatcactgcc tgatcatcac aaagtagaat
      961 tttcataaat agatcgtaga cgatggacga tagaccatgg aagtaaccat aggcatcgtc
     1021 aaataattag atcgtcagca ccactttggt gccttttccc tttccgcttt caatttttag
     1081 agtgccaccc gccaactcag cacgttcgcg catgccaacc aaaccaaagt gacctgtaga
     1141 gtctttgggt ttcatatcaa acccaatgcc atcatcttga atgactaatg tcgtgtgttt
     1201 atcgctagcc aaccgcacac tgaatttttt tgcttgtgaa tgtttggtca tgttctcaat
     1261 cgcttcctgc gtcacacgta ggatggtttg ctctacgtct gggctgagag aaggcatggg
     1321 atcttgcaaa tccagttcga ggtccagaga gaagcgagag gcggcggact ctgccgcctt
     1381 tttcacggcg agcctcaagc ccatatccac taaatcggat gaacgtaagg ctttcaaggc
     1441 gcggcgggtt tcgtccacac cttttcgagt cgcttcaagc gatgtgtcga tcatttcacg
     1501 agattgattc aggtcaatat cgaaatatgc tttggcagtt tcaagggaaa cggaaatggc
     1561 agtaagggaa tgtgcgaggg tgtcatgcaa ttcacgagca agacggttac gctcgcgact
     1621 caccgtaagc tgttccaacg tggacgcata gtgcgtgaga ttggcatttg cttcacgaag
     1681 tgactcacgt tgttttcgca aacgtgacac aatgaaactg ataaagaaac ccaccgctac
     1741 aaaactaatg gtacgaataa tggcgatgaa aatgtatacg gtaatgttgc gattatctga
     1801 tgggttgatt gcgaccagcc caagttcgat aaaagtagta acgatactaa aaagaaccac
     1861 atgaagaaac cgataattcc atgccacaag caccagtgct acgaacatca ccggcaattg
     1921 acgtaacgcc atcccttctg cattggaaag cggaccttgt ggaaagcgcg gaatgataaa
     1981 agcgttgatc acaatcggcg ccaccgatat aaccgataac aaaaatggat agtatatatc
     2041 ttccattttt gcttgaatat cactccaatg cgtaagagcg aggaaaaata aagccgttag
     2101 cccgttggca gtgtaaaaaa taaaaacagg gtagtgtggt ccacgcaaag gagagtttgg
     2161 taaatttcca atgggggtag gcggcaaagg ttggtttggc agggcaggtt gtaagcgagt
     2221 tgaatctccc aacaagtttt gcacctgagg catatacagc gcaatatcca tcagcgccat
     2281 ggcaagtaga aagccaagcc aaatccaggc ggcagtgcga agaatgcgag tggagtcatc
     2341 cgttgaatgc atatctgcat cttatcatga agggattttt catgggtatg acccgtgtaa
     2401 cgaccttgta aacccaccca tgacagcagt catgggtggg tcttgagaat cgtgatttgg
     2461 ggcactctgc tctttttagg tggaaaaata aaatacatag catgaaaaca atagcaactg
     2521 ttcctacctc cctccaaaaa caagaagtga cccgcttcgc gcgcttctta accgttggtg
     2581 cagtcggcac cctgctagat ttcagcattc tcaccttgct caaactggca ggaatgccca
     2641 cgttgttcgc gaactccctt tcgttcacgg caggtttgct taataacttt acatggaatc
     2701 gcatctggac atttagtgac acagccaaag ccgattggcg caagcaattg gcacaattta
     2761 ccttggtcag tttggcaggg ctggtcttga ataacttgat cgtgctttca ctagaaggcg
     2821 ttttagggaa tattcttggt c
//
LOCUS       DAOREF010000368         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24246_length_2836_cov_4.374685, whole genome shotgun sequence.
ACCESSION   DAOREF010000368 DAOREF010000000
VERSION     DAOREF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24246_length_2836_cov_4.374685"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PKI33_08465"
     CDS             complement(<1..1195)
                     /locus_tag="PKI33_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM37082.1"
                     /translation="MTGELKGQFNHPNSEQNNDTIFQDAVNALRSGDKPRAKELLTLL
                     LKTDQNNPTYWIWLSASVDANKERIYCLQTALKLDPENSIAKRGLILLGALSPDETIQ
                     PFPMNRPRVWEEKLLLAHEKPKERGVRVVMRSPLVRLGGLAIIGIGLCAVIYFGVILP
                     RWGTIQLAPTNTPGPSPTFTATPTIFGATAIPTQVFSGPTPLSAFLNATYTPTALYVN
                     TPRSAVSVDQFRIAKQAYDNKDWDVFIENMLLIEPLEPDSVDIPYYIGEAYRFKGDTT
                     SAARYYRDAMNMNPAFGPAYLGLARVSLMSNPDFNPEKLFNQAIEYDPNLGEAYLERA
                     RWLIRRGDYADALPDLELADKIMPGSPEVYLAYANSYLGDGDKEKALEYAQKAYEADI
                     LNLPTY"
     gene            complement(1205..1858)
                     /gene="amrA"
                     /locus_tag="PKI33_08470"
     CDS             complement(1205..1858)
                     /gene="amrA"
                     /locus_tag="PKI33_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNM37083.1"
                     /translation="MEIIETEVKKTPSQNAWRSLYSCEDAMSALLTNKERQTLLHIAR
                     VSIESAVKGNPQPTLEKDTLTPALRENGASFVTLTINGELRGCIGALEAYQPLAEDVC
                     EHAVAAALNDPRFPALREAELSRIQIEVSYLTKPQELQYSTSQDLLAKLHPHLDGVIL
                     KYGRRRATFLPQVWEKLPKPEEFLSQLCYKMGERQTLWQETKLQVYVYQVEEFHELN"
     gene            complement(1834..2325)
                     /locus_tag="PKI33_08475"
     CDS             complement(1834..2325)
                     /locus_tag="PKI33_08475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNM37084.1"
                     /translation="MTLDPEALRSAMRAWSAGVTVVTAVHEGEMHGMTVNSFTSISLT
                     PPLLIISLQQNTRTHELVMKSRAFGLTILSSEQSKVSDLFAGRMPEVEDRLATLKTET
                     LVTGSPLIVGGLAWLDCRVLQTYDAGSATLFIAEAVSARGTGSGDPLIYHNREYWKLS
                     KLK"
     gene            2505..>2836
                     /locus_tag="PKI33_08480"
     CDS             2505..>2836
                     /locus_tag="PKI33_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37085.1"
                     /translation="MSPKVRNILLGLAGLVLLAVLVYQIPAVKSRLEWRLDVFQIYVK
                     NVIDPVGPVPTALPVTPLPATPTIAITNTAVVQALPTVTATPTFPPLPAQAGITSPPY
                     EKQTANNCG"
BASE COUNT          743 a          687 c          677 g          729 t
ORIGIN      
        1 tatacgtcgg gaggttgaga atatcggctt cgtacgcttt ttgagcatat tccaatgcct
       61 tttctttatc accatcgcca aggtaggaat ttgcatacgc aagatacact tctggcgagc
      121 ctggcatgat cttgtctgca agttccaaat ctggcagggc atcggcataa tcgccacgcc
      181 gaatgagcca ccgagcacgt tcaagatacg cctcgcccaa atttgggtca tattcaatgg
      241 cttgattgaa taatttttca gggttgaaat ctgggttcga catcagactt acgcgggcaa
      301 gccctaaata tgccgggcca aaggcaggat tcatattcat tgcatcgcgg tagtaacgcg
      361 ctgcgctggt ggtatctccc ttaaaacgat aggcttcgcc aatgtaataa ggaatatcta
      421 ctgagtctgg ctcaagaggt tcgatcagaa gcatattctc aataaagaca tcccagtctt
      481 tattatcata ggcttgcttt gcgatacgga actgatccac tgagacggct gaacgtggag
      541 tattaacata caaagcggtt ggcgtgtacg ttgcattcaa aaaggcagaa agcggagtcg
      601 gtccgctaaa gacctgggta ggaattgctg ttgccccaaa gattgttggg gttgcagtaa
      661 acgtaggaga aggacctggg gtgttggtgg gagcaagttg aatcgtgccc caacgcggaa
      721 ggataacccc aaaataaatt acagcacaca aaccaatgcc aataattgcc aacccgccca
      781 gccgtaccaa cgggcttcgc atgacgacgc gcacgccacg ctcttttggc ttttcatgag
      841 caagcagtaa cttctcttcc cacacccgcg gacggttcat agggaagggt tgaatggtct
      901 catctggcga aagagctccg agtagaatca agccgcgttt ggcaattgaa ttttcggggt
      961 ccagtttaag agccgtttgc aaacaataga tgcgttcttt attggcatcc actgaggcac
     1021 ttagccaaat ccaatacgtt gggttgtttt ggtccgtttt taggaggagg gttagcaact
     1081 ctttggcgcg gggtttgtct ccgctgcgca gagcattcac ggcatcctga aaaatggtgt
     1141 cgttgttttg ttcgctatta ggatggttaa attgcccttt gagttcgcca gtcatgcgtc
     1201 cattttaatt caattcatgg aattcttcga cctgatatac ataaacttgc aattttgtct
     1261 cctgccaaag agtttggcgc tcccccatct tgtagcaaag ttgacttaga aattcttccg
     1321 gcttggggag cttctcccat acttgtggca gaaaggtggc gcggcggcgt ccgtatttca
     1381 aaatgacccc atccagatga gggtgtagtt tggcaagtaa atcttggcta gtggagtact
     1441 gcaactcctg cggctttgtg agataagaaa cttcgatttg aatacgactc aattccgctt
     1501 cacgcagggc tggaaaacgc gggtcgttta acgccgccgc aacggcatgc tcacagacat
     1561 cttctgcaag gggttgatac gcctccaaag cgccaataca accgcgcagt tcgccattga
     1621 tcgtgagggt cacaaaagat gctccgtttt cacgcaaagc aggtgtaagc gtatcttttt
     1681 caagagttgg ttgagggttc ccctttacgg cactctcaat agacactctg gcaatgtgca
     1741 aaagagtttg tctttcctta tttgtcaaca aagcagacat ggcatcctca catgaataca
     1801 aagaacgcca agcgttttgg cttggcgttt tctttacttc agtttcgata atttccaata
     1861 ctcacggttg tgatagatca agggatcgcc gctgccagtt ccacgagccg aaactgcttc
     1921 ggcaataaat aatgtagccg aacccgcgtc ataagtttgg aggacgcggc aatccaacca
     1981 ggcgagtccg cctacgatca ggggagagcc ggttacaaga gtctcggtct tcaacgttgc
     2041 taggcgatct tccacttcgg gcatgcgccc ggcaaacaaa tccgagacct tcgattgttc
     2101 agaagaaaga atagttaaac caaaggcgcg cgatttcatc accagctcat gcgtacgagt
     2161 attctgttgc aaagaaataa tgagcaaggg cggggtcaac gaaatagatg taaatgaatt
     2221 aacggtcatg ccgtgcattt cgccttcgtg cacagcagta accacagtga cacctgccga
     2281 ccacgcacgc atggcagatc gcagggcttc agggtctagg gtcataacag gggaattctc
     2341 catagatatt tctcgttttc atcataattg ccgtaaaaga gcaagtcaag ccaaaaaccg
     2401 catagtttca gcaattttga atactctttg caagacattg attcaatggc aaaacaaaaa
     2461 cttaccttaa tcttccttta tgcttgcgtg gtacactcac gcgcatgtct ccaaaagtca
     2521 gaaatatcct actagggctg gcaggtttgg tactgttagc tgtgttggtt taccaaattc
     2581 ctgctgttaa atcccgcctt gaatggcgtt tggatgtgtt tcaaatttat gtaaaaaacg
     2641 tcatcgaccc agtgggacct gtccctaccg cattgccagt cacgccattg cccgctacac
     2701 cgacgattgc tataaccaat acagccgtgg tgcaagcctt accaacagtc acagcaacac
     2761 ctacattccc gcctttgcca gcacaagccg gtatcacttc accaccttat gaaaagcaaa
     2821 ctgccaacaa ctgcgg
//
LOCUS       DAOREF010000369         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24269_length_2835_cov_3.330935, whole genome shotgun sequence.
ACCESSION   DAOREF010000369 DAOREF010000000
VERSION     DAOREF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24269_length_2835_cov_3.330935"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="PKI33_08485"
     CDS             <1..1901
                     /locus_tag="PKI33_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM37086.1"
                     /translation="EAIFAYFLGVSGFSVSLPNILAGVFGIGLLYSMVKKYMGELAGI
                     VAALVMAITPVFVATNRNNTMDGMLVFTLLLAAWAFIHATETGKARWLMVGAFIVGLG
                     FNIKMLQAFLPLPAFYALYFFGSKEGWLRKIINLGIATVLLLVVSLSWAIAVDLTPAE
                     NRPYIGSSENNTVMGLIFGHNGISRLENNRGGGNAPQTGTQPEQPTNQQTQPQGNPGQ
                     GQFQGPPPAALEACSGQTQGSACSFTTQNGNTINGSCITPPNISELACAPQGMIPQNG
                     QAPAGGPTGAPNGGPNGQNGNTPFGNETGSPSALRFFTSPLSKQMSWLLPFALISVLI
                     ALFGSRIQLPIVSGVHKALLLWGGWLLTCVVFFSMVSGIFHAYYAIMLAPALGAMVGV
                     GFAQLYQWGKNSNWVLVGLIAAVVVTLGFQIFAATQYNEFAWWMLGTGILLGLGILLM
                     SVSRRAAYAVILSAALVIPAYWSVMTVTNGSNNNLPTAYEGQNRNGQDGFLASPQQQN
                     QGNRGNDRSVNTELLAYLEANTVGMKYLMAVPSSQQGSQYVIETGRPVLYMGGFSGGD
                     AVVTVDDLKELVANGELRYILYGGDRGNQEITSWLNSSCAIVNDFNDLSQAQQGPQNQ
                     AMTLYSCR"
     gene            complement(1933..2262)
                     /locus_tag="PKI33_08490"
     CDS             complement(1933..2262)
                     /locus_tag="PKI33_08490"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNM37087.1"
                     /translation="MSALSIWLLFVAIGAGTFTLRFLFIYLFGKVEMPTWLSRALRFV
                     PAAALAALVFPALTHPAGHLDISLTNFRLLAGLGGALVAWRTRNVLLTILVGMALLWS
                     LQAAFGM"
     gene            complement(2259..2576)
                     /locus_tag="PKI33_08495"
     CDS             complement(2259..2576)
                     /locus_tag="PKI33_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37088.1"
                     /translation="MGRRYFGAALIIWVAWQVSTVIGTLLGALVPASLGFDFAFPLSF
                     MALMFGALRDRPTVVAALTGGFIAVLAKSLPYNTGLVLATFLGIIAGYVAESLKLKEE
                     AAL"
     gene            complement(2521..>2835)
                     /locus_tag="PKI33_08500"
     CDS             complement(2521..>2835)
                     /locus_tag="PKI33_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37089.1"
                     /translation="LNWQMAFLRGFKPHFARQLDVEKWWSLQMEFLTGRDERGLWTLA
                     ESWNKLDQLLRVPIQVRRQKQDLPTLAEASLTSVIAEWDYTRHGPALLRRGVDHLGGV
                     AG"
BASE COUNT          699 a          782 c          691 g          663 t
ORIGIN      
        1 tcgaagccat cttcgcgtat tttctgggcg tgagcgggtt tagtgtttca ttgccgaata
       61 tccttgcggg tgtattcggt atcgggttgc tctacagcat ggtcaagaaa tacatgggcg
      121 agttagcagg gattgttgcg gcactcgtca tggcgatcac cccggtcttt gttgctacca
      181 accgcaacaa caccatggac ggtatgttgg tcttcacact cttgcttgct gcgtgggcgt
      241 tcatccacgc cactgaaacc ggtaaagcac gctggctcat ggtaggcgct ttcatcgtcg
      301 gcttgggttt caacatcaaa atgctgcaag cctttttacc tttgccggct ttctatgcct
      361 tgtacttctt tggctcaaaa gaaggctggc tcagaaaaat catcaacctc ggcatcgcta
      421 ccgtcctctt actagtggtc tcactttcgt gggcaatcgc tgttgacctc acccctgccg
      481 agaatcgccc ctacatcggc tccagtgaaa ataacacggt gatgggattg atcttcgggc
      541 ataacggcat ttcacgcctt gagaacaacc gtggaggcgg aaatgcgcct caaacgggca
      601 ctcagcctga gcaacccacc aatcaacaga cacagccgca agggaatccc ggtcaagggc
      661 agttccaagg tccacctccg gctgcgctgg aagcctgctc tggtcaaacg caagggtctg
      721 cctgttcatt caccacgcaa aatggcaata ccatcaatgg ttcttgtatt accccgccca
      781 atatttcaga actagcttgt gctcctcaag gaatgatccc tcaaaacggg caagctcctg
      841 caggtggacc cactggggcg ccaaatggcg gtccgaatgg gcagaacggc aacaccccat
      901 ttgggaatga gaccggctct ccaagtgctt tgcgcttctt cacctcacct ctttccaaac
      961 aaatgtcgtg gttgctacca ttcgcattga tcagcgttct gattgcctta ttcggctcac
     1021 gcattcaact ccccatcgta tctggggtac ataaagccct actcctctgg ggcggatggc
     1081 tactcacatg tgtagtcttc tttagcatgg tttctggcat cttccacgct tactatgcca
     1141 tcatgcttgc cccggcatta ggagccatgg ttggtgttgg ctttgcccag ctttatcaat
     1201 ggggcaagaa ctcgaattgg gtcttggttg gtctgatcgc agcggttgtt gtcacacttg
     1261 gtttccaaat ctttgccgcc acgcaataca acgaatttgc atggtggatg cttggaacgg
     1321 gcatcctgct tgggttggga atcctgctca tgtccgtttc gaggcgtgcg gcgtatgccg
     1381 tcatcctcag tgcggctttg gtcatccccg cttattggag tgttatgacc gtcaccaatg
     1441 ggtcgaataa caacctcccc accgcctatg aaggtcaaaa ccgcaacgga caagacggct
     1501 tccttgccag cccgcaacaa caaaatcaag gcaaccgcgg caacgatcgc agtgtaaata
     1561 cagaactttt ggcatacttg gaagcaaata cggtaggcat gaagtatctt atggcagttc
     1621 catcctcaca acagggttca caatatgtaa tcgaaaccgg gcgcccggtc ttatacatgg
     1681 gtggtttcag tggcggtgat gccgttgtaa cagtggatga tctgaaagaa ttagttgcca
     1741 atggagagtt gcgttacatt ctctatggcg gcgatagagg taaccaagaa attaccagtt
     1801 ggctaaatag ctcatgtgcg attgtgaatg atttcaatga tttatctcaa gcgcagcagg
     1861 gtccacaaaa tcaagccatg accttatact cttgcagata ataaaaagcc ccgagataat
     1921 ctcggggctt ttttacatcc caaatgctgc ttgaagagac cacaacaaag ccatgcccac
     1981 caaaatggta agcagaacat ttcttgtgcg ccacgccacc aatgccccac caagccctgc
     2041 caagagacgg aaattcgtga gcgaaatatc aaggtgcccc gcagggtgag tcaacgctgg
     2101 gaagaccaac gccgccaacg ctgccgcagg gacgaacctt aacgcccggc tcaaccaagt
     2161 gggcatctca actttgccaa agagataaat aaacaaaaac cgaagcgtaa atgtgcccgc
     2221 tccaatcgcc acaaagagaa gccaaatcga taatgcgctc atagggctgc ctcctctttt
     2281 agcttcagac tttcagcaac atacccggca ataattccaa gaaaggttgc caataccaaa
     2341 ccagtgttat acggcaagct tttcgccaac acggcgataa aaccgccggt caatgctgct
     2401 actactgtag ggcggtctcg caaagctccg aacatcaacg ccatgaaact gagcggaaag
     2461 gcaaagtcaa agccgaggct ggcaggtaca agagcgccta ataatgtccc gatcacagtg
     2521 ctaacctgcc acgccaccca aatgatcaac gccgcgccga agtagcgccg gcccatgccg
     2581 cgtgtaatcc cattctgcaa tgacgctggt cagcgatgcc tccgcgagag ttggcaggtc
     2641 ttgtttctga cgacgcacct ggattgggac gcgcagcaat tgatcgagtt tgttccagct
     2701 ttccgccagc gtccacaaac cgcgttcatc gcgtccggtc aggaattcca tttgcagcga
     2761 ccaccatttc tcgacgtcga gctgccgtgc aaagtgcggt ttgaatcctc ggagaaaagc
     2821 catctgccag ttcag
//
LOCUS       DAOREF010000370         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24329_length_2831_cov_5.016571, whole genome shotgun sequence.
ACCESSION   DAOREF010000370 DAOREF010000000
VERSION     DAOREF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24329_length_2831_cov_5.016571"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKI33_08505"
     CDS             <1..248
                     /locus_tag="PKI33_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37090.1"
                     /translation="ELKIGVVVMESPNGFPLHETNIYLVAPSGDVVWKAEKPSANVLF
                     TKMKLNEDGSISTFTNSGQFCDINGENGKVISSSNFS"
     gene            267..791
                     /locus_tag="PKI33_08510"
     CDS             267..791
                     /locus_tag="PKI33_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM37091.1"
                     /translation="MPLHFTKLANPIPEEVYSAFTKWENDPALVHLTRPNQTQADIDR
                     KSEVDSFELDARISYQMTYLIYLDSQLVGEMSYQVDPQHLYKKETGTAWISITIGEES
                     ARGKGIGKLAMQYLEEEIHNAGYKRIELGVFEFNEPAQKLYQKMGYTEIGRIPNFTFW
                     QNKMWTDIRMEKYI"
     gene            791..1075
                     /locus_tag="PKI33_08515"
     CDS             791..1075
                     /locus_tag="PKI33_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37092.1"
                     /translation="MIKGFLNLPWFVWAGLALVVALIYTFWVPQKIAPDISGIRLLIV
                     RWAHPLTWYLLALNFILRGISPEWGGIANMVAAAGGLMYMLFMLMTFVIK"
     gene            1077..1355
                     /locus_tag="PKI33_08520"
     CDS             1077..1355
                     /locus_tag="PKI33_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37093.1"
                     /translation="MEKPPFSFVLIFLLFSFLFISNSYKLWFKTDEYYKDLYASLTNE
                     RIPFPFKSFFLARLEKKQSWLFWQKAFSLLGIVAVVGMDVLVVMAYLG"
     gene            1440..2210
                     /locus_tag="PKI33_08525"
     CDS             1440..2210
                     /locus_tag="PKI33_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNM37094.1"
                     /translation="MKIIACIKQVPDSEAKVKAEGGQVSWGDAPLVINPFDEYAVEGA
                     LQQKEATGGTVTALCVGPESAKEALKHALAMGADEAILVSDAALNDLDTVGAAKVLAA
                     AIQKIGGADMVVFGRQTLDNGAGLTPPQTARLLGYPMLGLAGSIKVEGSAVTVERVLE
                     EGRQIMKANLPAVLSVVQSIGEPRYPSFMGIRKASKANIPVWSLADLGISAPAAVVTR
                     AELIPPPSDTRTVEMITGESPAEIAEKLADKIIAEKVL"
     gene            2207..>2831
                     /locus_tag="PKI33_08530"
     CDS             2207..>2831
                     /locus_tag="PKI33_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNM37095.1"
                     /translation="MKTFVYIDHFKGDVQPASWEALGLAKTFGTAVALVFGAGVDEVA
                     KAAVEFGADEVLVADDASLADYRAETYASTLSALASSQSPNLILLPTTSRTRELAAMA
                     AVDLNTGVLTDLIGLEANGDSFVATRPIYEGKLMEKTVCSGKPVMATIRGRAFAKPER
                     GSGNGTITKVAAAGEAKSSVEGYSASESAVNLGDAGIIVSGGRGVSNN"
BASE COUNT          722 a          707 c          737 g          665 t
ORIGIN      
        1 ttgagttgaa aataggtgtt gtcgtgatgg aaagcccaaa tggcttccca ttacacgaga
       61 ccaacatcta tttggttgct ccaagcgggg acgttgtttg gaaggcagaa aaaccttcag
      121 ccaatgtgct tttcaccaaa atgaagctaa atgaagatgg cagtatttcc actttcacga
      181 acagtggaca gttttgcgac attaacggcg agaacggcaa ggtaattagt agttccaatt
      241 tcagctaata gcaggaacaa gtgcggatgc ccctccattt cacaaaacta gcgaacccta
      301 tcccggaaga ggtctattct gcttttacca aatgggaaaa tgatcctgca cttgtccacc
      361 ttactcgacc caaccaaact caggcagata tcgacagaaa aagcgaagta gattcgtttg
      421 aattggacgc acgaatttct taccaaatga cctacctaat ttatttggat agtcaactgg
      481 taggtgaaat gagttaccaa gtagacccgc agcatctcta caaaaaagag accggcaccg
      541 catggatcag catcaccata ggtgaagaat ctgctcgagg taagggaatt ggcaaactgg
      601 caatgcaata tctggaagaa gaaattcata acgcaggtta caaacgtatt gaattaggcg
      661 tttttgaatt caatgaacct gctcaaaagc tatatcaaaa aatgggatat accgaaatcg
      721 gcagaattcc caacttcacg ttctggcaaa acaaaatgtg gacggacatt cgcatggaga
      781 aatacatctg atgatcaaag ggttccttaa cctgccgtgg tttgtttggg ctgggctcgc
      841 tctggttgtt gcgcttattt ataccttttg ggttcctcaa aaaatcgcgc cagatataag
      901 cgggattcgc ctgttaatcg tccgctgggc acaccctcta acatggtact tgctcgccct
      961 caatttcatc ctgcgcggta tttcgccaga gtggggtggt atcgccaata tggttgccgc
     1021 tgcaggtgga ttgatgtata tgttgtttat gcttatgacc tttgtgatca aataatatgg
     1081 aaaaaccacc ttttagcttc gtccttatct ttcttttatt tagttttttg tttatttcga
     1141 acagctataa actttggttc aaaacggatg agtattacaa agacctctac gccagcctca
     1201 ctaacgaacg aattcccttt ccatttaaga gtttctttct agcacggctg gagaaaaaac
     1261 aaagctggct gttctggcaa aaggcgttta gtttgttggg cattgtggca gttgtcggta
     1321 tggatgtttt ggtagtcatg gcgtatttgg gataagaaat taaccacaaa gtgtcacaaa
     1381 ggaaagacac tttgtgaccc gtcgtggtaa agaattgaat tcatatcaag gagcttaact
     1441 tgaaaatcat tgcatgtatc aagcaagtcc ccgactcgga ggcgaaggta aaagccgaag
     1501 gcgggcaggt ctcatggggc gacgcgccgc tggtcatcaa cccgttcgat gaatatgcgg
     1561 ttgaaggcgc gttacagcaa aaggaagcca caggcggaac cgtcacagcc ttgtgcgtcg
     1621 gacccgaatc cgcgaaggag gcgcttaagc acgcgcttgc catgggtgca gatgaagcca
     1681 tcctcgtttc ggatgcggca ttgaacgacc tcgacaccgt cggtgcggcg aaagttctcg
     1741 cggctgccat ccaaaagatc ggcggcgcgg atatggtcgt ctttggtcgg cagactctcg
     1801 acaatggcgc cgggctcact cccccgcaga cggcacgcct cctcggttat ccaatgcttg
     1861 ggttggctgg cagcataaag gtcgaaggaa gcgccgtgac cgttgaacgc gtcctcgaag
     1921 aaggtcggca gatcatgaaa gcgaatctgc ccgcggtgtt gagcgtcgtg caaagcattg
     1981 gcgagccgcg ctacccgtcg ttcatgggca tccgcaaagc gtcgaaggcg aacatccccg
     2041 tttggtcgtt ggcagacctc ggcattagcg cccccgccgc agtggtgacc cgcgccgagt
     2101 tgatcccccc gcccagcgac acccgcaccg tggaaatgat caccggtgaa agccccgccg
     2161 agatcgccga aaaactcgcc gacaaaatca tcgcggagaa ggtgctatga aaacgtttgt
     2221 ttatatcgat cacttcaaag gggatgttca gcccgcttca tgggaagcgc ttggtttggc
     2281 gaaaacgttt ggcacagccg tcgcgttggt ctttggcgca ggtgtagatg aagtagccaa
     2341 agctgcggtt gaatttggcg cggatgaagt cctcgttgcg gatgatgcct cgctcgcaga
     2401 ttaccgtgct gagacgtatg cttccacact ctccgcgctc gcctcttccc aaagcccgaa
     2461 cttgattctg ctcccaacca cttcgcgaac ccgtgagctt gccgccatgg cagccgtgga
     2521 cttgaacacc ggcgtgctga ccgatctcat cggtcttgaa gcgaacggcg attcattcgt
     2581 cgccacccgc ccgatctacg aaggcaagtt gatggaaaag accgtctgct ctggcaagcc
     2641 tgtcatggca accatccgcg gacgcgcctt cgccaagccg gaacgtggat caggcaatgg
     2701 aacgatcacc aaagtagcgg cggcgggtga agcgaaatcc agcgtcgaag gctactccgc
     2761 ctctgaaagt gccgtcaact tgggcgacgc aggcatcatc gtctctggcg gacgcggcgt
     2821 ttcgaacaac c
//
LOCUS       DAOREF010000371         2830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24346_length_2830_cov_3.520000, whole genome shotgun sequence.
ACCESSION   DAOREF010000371 DAOREF010000000
VERSION     DAOREF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24346_length_2830_cov_3.520000"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKI33_08535"
     CDS             complement(<1..1166)
                     /locus_tag="PKI33_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM37096.1"
                     /translation="MVKTKFVEVSRSFIVIAILIWLAIAALGTIFLPGETGEIREMFA
                     FSQIIPILVAAFYFGQTGGLLAAFAASVVSGSLVIANIDNIDSIFVQRVMFQIISFNS
                     VALLTSFLSDQAKAHRRRLTRQVERLNALRAIDKAIISGGDLSNTLYILLESLTRLLN
                     VEAASIFLFDTKTEQLRLSASLGFFGEYPHQALCNLGEGMVGQAAAEKQTIYHHNLEK
                     VDKQIIPAVKGEGVMDYYAVPLFANDRLRGVLEVFDRAPRLDSEWKSYLETLAGQAAI
                     AIAQSTLLGELKDANKELSEAYEETLKGWSRALNLRDKETDHHTQRVVELSVQLAGRM
                     GLRGEQLIQFERGAILHDIGKMGIPNEILLKPGKLTEEEWIIMRKHPLYAQMLIA"
     gene            complement(1160..1657)
                     /locus_tag="PKI33_08540"
     CDS             complement(1160..1657)
                     /locus_tag="PKI33_08540"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNM37097.1"
                     /translation="MQKDRSYIGLILFTLAYPAAHYLVGFIPNPFVPEANLAINMIFP
                     ILAGYFYGPISGAIAGFLGTGLSAILTADVYDALAILPHTIMGLVAGLAGNSRQQFLA
                     ACSVLLGHALNILFFSRFGYLLIERPPILILGLLTETTVDVVAVILFIVLLHRKLYRV
                     DDERW"
     gene            1779..2387
                     /locus_tag="PKI33_08545"
     CDS             1779..2387
                     /locus_tag="PKI33_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM37098.1"
                     /translation="MDETALIASAQQGDLNAFNTLILHYQDAVFNTAVRILGDDDMAQ
                     DAAQDAFISAFQSIKSFRGGSFKAWILRTVTNACLDELRRQKRRPTTPLEPETHDGEE
                     MDSPKWLADSSMTPAQKAEADELEHAIQHCLDALPTEFRTAVVLADVQGMDYTEVATA
                     IRAPLGTIKSRIARARLRLRECLRSFEELLPSSFRLEEENAQ"
     gene            2384..>2830
                     /locus_tag="PKI33_08550"
     CDS             2384..>2830
                     /locus_tag="PKI33_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37099.1"
                     /translation="MKNFYDVETLSAYLDGQLDASNAARLEARLKTDAELASALRDLR
                     AARGILKKLPARKAPRNFTLTRQMVGLKPPLPRTYPLLRFATAFAAILFLFSFTANTL
                     VPMFNFGAAAPAYGLGSGGGCDACGGGGPESAPMAAAATEAAATEAP"
BASE COUNT          816 a          738 c          595 g          681 t
ORIGIN      
        1 gcaattagca tttgagcata aagcggatgc tttcgcatga tgatccattc ttcttcagtc
       61 agtttcccgg gtttgagcaa aatctcattt ggaattccca tcttcccaat atcatgcaag
      121 atcgcccctc gctcaaactg gattaactgc tcgcctcgca atcccattcg tcctgccagc
      181 tgaaccgata actccaccac ccgttgggta tggtgatccg tttctttatc gcgcaaattc
      241 aaagcacgcg accaaccctt caaggtttct tcgtaggctt ccgataattc tttatttgca
      301 tcttttagct cccccagcaa tgtagactgt gcaatagcaa tcgctgcctg cccagccaag
      361 gtttcaagat agcttttcca ttccgaatcc aaacgaggag cacggtcaaa aacctccaac
      421 acccctctca aacggtcatt tgcaaacaag ggcacagcat aataatccat gaccccttcg
      481 ccttttacag cagggattat ctgcttatct actttttcca ggttatgatg ataaatcgtc
      541 tgtttttcag cggctgcttg ccccaccatg ccttcaccca gattacacaa cgcttggtgc
      601 ggatattcgc caaaaaaacc aaggctagcg cttaaacgta attgttctgt tttagtgtcg
      661 aacaaaaata tggaagcggc ttctacatta agcagccgtg taagactttc cagtaatatg
      721 taaagggtat tgcttaggtc tcccccagaa ataatggctt tatcaatagc tcgcaaggca
      781 ttgagacgct ccacctgccg cgtcaacctt ctgcgatgcg ccttggcttg atcagacaga
      841 aagctggtta gcaaggctac agaattgaac gatatgatct ggaacatcac acgttgaaca
      901 aagatcgaat caatattgtc aatgttcgcg attactaaag aaccgcttac tacgctggca
      961 gcaaaagccg ctaaaagccc gcctgtctga ccaaaataaa aagctgccac caaaatggga
     1021 atgatctgag aaaaggcaaa catctcccga atctcgcccg tctcaccagg aagaaagatc
     1081 gttcccaagg cagcaattgc cagccatatc aaaatagcaa tgacaataaa agatcttgaa
     1141 acttcaacga atttagtttt caccatcgtt catcgtctac acgatatagc tttcgatgca
     1201 aaagtacaat gaagagaatc acagcgacaa catccactgt tgtttcagta agcaagccca
     1261 gaatgagaat gggaggtctt tcaataagga gatagccaaa acgcgaaaag aaaaggatgt
     1321 ttagcgcatg tcccaacaac acagaacaag ccgccaagaa ttgttgacgc gaattccctg
     1381 ccaaaccagc aaccaaaccc attatggtat gtggcaaaat cgcgagcgca tcatagacat
     1441 cagcagtaag aatggcagaa agcccggtcc ctaaaaagcc tgcaattgca ccgcttatag
     1501 gaccataaaa ataccccgca agaatcggaa aaatcatatt aatagcaagg ttggcttcag
     1561 ggacaaacgg attggggata aaccccacaa ggtaatgagc cgctgggtat gccagcgtga
     1621 ataagatcaa tcctatatag gatctgtctt tttgcatctg caaattatag tagatagata
     1681 catctttttg tattgcaaaa tataaaatct gttatatgta tacaagaaca ctccaaaatt
     1741 gtatagtgaa atgaaaagac cgggtaaaat tacagtctat ggatgaaact gcattaattg
     1801 cctctgccca acaaggcgac ctgaacgcgt tcaatacgct catcctgcat tatcaggatg
     1861 ctgtcttcaa cacagctgtg cgcatccttg gggatgatga tatggcgcaa gatgctgcac
     1921 aagatgcttt catctcagca ttccaaagca tcaaatcatt tcgcggcggc tcgttcaaag
     1981 cttggatcct gcgcactgtg acgaatgcct gtctcgatga attgcgccgc cagaaacgcc
     2041 gccccacgac tccgcttgaa cctgaaaccc acgatggtga agaaatggac tctcccaaat
     2101 ggctagcaga ttcgagcatg acaccggcgc aaaaagcaga agccgatgaa cttgaacatg
     2161 ctattcaaca ttgcctcgat gcgttgccca ctgaattccg cacagcagtt gtgcttgccg
     2221 atgtgcaagg catggattac accgaagttg caaccgcgat ccgtgcgcca ttgggcacca
     2281 tcaaaagcag aattgcacgc gcacgattaa ggttacgtga gtgtttacgt tccttcgagg
     2341 aacttttgcc ctcctcattt cgtctggaag aggagaacgc tcaatgaaaa atttttacga
     2401 tgttgaaacc ctctctgcat atttagatgg acaactcgat gcttcgaatg cggcgcgact
     2461 agaagcccgc ctcaaaaccg acgccgagct tgcctctgct ttaagagacc ttcgcgctgc
     2521 tcgtggcatt ctcaaaaagc tgccagcacg caaagctcct cgcaacttta cccttacccg
     2581 ccaaatggtg gggctcaaac cacctctgcc gcgcacctat ccattgctta ggtttgcaac
     2641 tgcatttgca gccattctct tcctattttc gttcacagca aacacactcg tcccaatgtt
     2701 caactttggt gcagcagccc ccgcatatgg attaggttct ggcggcggct gtgatgcttg
     2761 tggcggcggt ggacccgaat ctgctccgat ggcagcggca gccacagaag cagccgctac
     2821 agaagcccca
//
LOCUS       DAOREF010000372         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24362_length_2829_cov_5.993872, whole genome shotgun sequence.
ACCESSION   DAOREF010000372 DAOREF010000000
VERSION     DAOREF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24362_length_2829_cov_5.993872"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..417
                     /gene="rpsF"
                     /locus_tag="PKI33_08555"
     CDS             133..417
                     /gene="rpsF"
                     /locus_tag="PKI33_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNM37100.1"
                     /translation="MRNYELMCIIQPDLDETAMNGVLDKVKGWITEAGGSIDKAEVWG
                     RRRMAYSINKHREGQYVLLNVSMSPAATSDLERNLRYQESIMRHMLSVVG"
     gene            454..861
                     /locus_tag="PKI33_08560"
     CDS             454..861
                     /locus_tag="PKI33_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNM37101.1"
                     /translation="MSRGLNKVQIIGHLGKDPEMRYTPSGKPVTTFTVAVSRTWNSAD
                     GERHNETEWFNVVAWGTLAEICKQYLVKGQQVYVEGRLQTRRWDDKEGIKHTSVEIVA
                     NEMMMLGDRREANNQPDASPAESNGAADEEFPF"
     gene            912..1205
                     /gene="rpsR"
                     /locus_tag="PKI33_08565"
     CDS             912..1205
                     /gene="rpsR"
                     /locus_tag="PKI33_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNM37102.1"
                     /translation="MSDERPERPERSGGEGGGERGDRKFFAKPKFCQFCADKTLTIDY
                     KKVDLLRKYVTEEGSLRPRRQTGACAKHQRLVAAAVKQARHVALLPFANRGVD"
     gene            1311..2744
                     /locus_tag="PKI33_08570"
     CDS             1311..2744
                     /locus_tag="PKI33_08570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNM37103.1"
                     /translation="MTNESGKKPAMHKKHVARLERELQQTRLILYTFFGILGVVAVLL
                     IYGWLDVNYLQLNRAVATVNDNKIALKDFEARTRLRRQQLIDNYNQYTQYQQYFGMDM
                     SSQLNSIETQLNTPELVGTSVLDAMIDEEIIRLEAEKRGITVSEEEIDKKMEEGFNFY
                     PNGTPTPSLTPTPADLPTDPAELLQFITATPDVTATPLFTATAEFTATVESQPTAEGD
                     ATATLAPTNTAEPTVTPTATLGPTSTPEPTATPFTREAYEVLLGKTKDNITKYGFDET
                     YIREFFRNQILREKLLEVIAADVKPFEEQVRARHILVADEQTAKDLIARLQAGEDFAD
                     LARQYSSDSSSEQGGDLGWFGKGQMVAEFETAAFALQNPGDFTTEPVASQFGYHIIQL
                     MAKQERPLSASEVQNAKETALQDWLTKARDTDYTVETFDFWKQRVPSTPNFITAATES
                     AAAQKTAQAESVATLDAKNSAAATPTP"
BASE COUNT          761 a          863 c          656 g          549 t
ORIGIN      
        1 cccgtaaaag tgattcctgt ggtaaacttt cgccgcgaaa ttaggtacgt tacatttcta
       61 tacgtgcctt tccgttcccg gcaaacccac ggtttgaaca ggaacaaacc catggaaagg
      121 aggttttccc acatgcgtaa ttatgaactg atgtgcatca ttcagccaga cctggatgag
      181 accgccatga atggcgtgct cgacaaggtg aaaggttgga tcactgaagc tggcggtagc
      241 atcgacaaag ctgaggtttg gggtcgccgt cgtatggcgt attccatcaa caagcaccgc
      301 gaaggtcaat acgttctctt gaacgtgtcc atgagccccg ccgccacatc ggaccttgaa
      361 cgcaacctgc gttatcaaga gtccattatg cgacacatgc tttctgtagt tggataatcc
      421 gtttttgccc ggcattctac ttaggagtga cttatgagcc gaggacttaa taaagtacaa
      481 atcattggac atttagggaa agaccccgaa atgcgctaca cccccagtgg caaacccgtc
      541 accaccttca cggtggcggt cagccgcacc tggaacagcg cagacggcga gcgccacaac
      601 gaaaccgaat ggttcaatgt cgtggcgtgg ggaacgcttg ctgagatctg caagcaatat
      661 ctcgtaaaag gtcagcaggt ctacgtagaa ggacgcctgc aaacccgccg ctgggatgac
      721 aaagaaggca ttaaacatac cagcgtagag atcgttgcca atgaaatgat gatgctcggc
      781 gaccggcgcg aagcgaacaa ccagccagat gcctcccctg ctgagtcaaa tggcgcagca
      841 gatgaggaat ttccgtttta gttcttgcca ttctgaacaa aagctcagag tgacatcaat
      901 tggagtaacc catgagtgac gaacgacccg aacgacctga acgctcaggc ggcgaaggcg
      961 gcggcgaacg cggtgatcgc aaattctttg cgaagcccaa gttctgccag ttctgcgccg
     1021 acaaaaccct gaccatcgac tacaaaaaag tcgaccttct tcgcaagtat gtaaccgaag
     1081 aaggcagcct gcgcccgcgc cgccaaaccg gcgcctgcgc caagcaccaa cgtctcgttg
     1141 cggcggcggt gaagcaagca cgccacgtag cgttgctgcc cttcgccaat cgcggcgtcg
     1201 actaaacatc gaactaacgc atcggggcat gggcaccagt ccatgccccg aatttatatc
     1261 cctgcaaaac acaaacaggg gcaatccctt tattacaaag cgagtatccc atgaccaacg
     1321 aatcgggcaa aaagcccgct atgcataaaa agcatgttgc ccgcctcgaa cgcgagctgc
     1381 agcaaacccg cctcatcctt tacaccttct tcggcatcct gggcgtggtc gctgtcttgc
     1441 tcatctacgg ctggctcgat gtgaactacc tgcagttgaa tcgtgcggtt gccaccgtaa
     1501 acgacaacaa gatcgcgctc aaagattttg aagcccgcac ccgcctccgc agacaacaac
     1561 tgatcgacaa ctacaaccaa tacacccaat accagcaata cttcggtatg gacatgagct
     1621 cacagctcaa cagcattgaa acccaattaa acacccctga attggttggc acctctgtct
     1681 tagacgccat gatcgacgaa gagatcatcc gcctcgaagc agaaaaacgc ggcatcaccg
     1741 tcagtgaaga agagatcgat aagaaaatgg aagaaggctt caacttctac cccaacggca
     1801 cccccacccc ttctctcacc cccactccag ccgacctgcc caccgacccc gcagaacttt
     1861 tgcaattcat caccgccacc cccgatgtga ccgctacgcc tctcttcacg gcgacagctg
     1921 agtttacagc cacggttgaa tcccagccga cggcggaggg cgatgcgacc gctaccttgg
     1981 cgcccaccaa cactgccgaa cccaccgtca cccccaccgc cacgcttggt cccacatcca
     2041 ccccagaacc tacggctacc cccttcaccc gcgaagctta tgaagtgttg cttggcaaaa
     2101 ccaaagacaa cattaccaag tatggtttcg atgaaaccta cattcgcgaa ttcttccgca
     2161 accaaatttt gcgcgaaaaa ttgttggaag tcatcgccgc agatgtgaag cccttcgaag
     2221 aacaagtccg cgcgcgccac attctcgttg ccgatgaaca aaccgccaaa gacctgatcg
     2281 cccgcctgca agccggtgaa gacttcgccg acctcgcccg ccaatactcc agcgatagct
     2341 cctctgaaca aggcggcgac ctcggctggt ttggcaaagg acagatggtt gctgaatttg
     2401 aaaccgccgc tttcgctttg cagaaccccg gcgatttcac cacagaacct gttgcctcgc
     2461 agttcggcta tcacattatc caattgatgg cgaagcaaga acgcccgctc agcgccagcg
     2521 aagtgcagaa cgccaaagaa accgctctgc aagattggtt gaccaaggct cgcgacacag
     2581 actacaccgt tgaaaccttc gacttctgga aacagcgcgt tcctagcacc ccgaacttca
     2641 tcaccgctgc caccgagtct gctgcggctc aaaagaccgc tcaagctgag tctgttgcca
     2701 cactcgatgc aaagaacagc gcggctgcaa cacccacccc gtaaagaatt acatcaaata
     2761 aaaaatgctc cagaaaatct ggagcatttt ttatttacag agcaagtcat tcacccaata
     2821 ttgcgccat
//
LOCUS       DAOREF010000373         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24449_length_2823_cov_4.630419, whole genome shotgun sequence.
ACCESSION   DAOREF010000373 DAOREF010000000
VERSION     DAOREF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24449_length_2823_cov_4.630419"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKI33_08575"
     CDS             <1..1039
                     /locus_tag="PKI33_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM37104.1"
                     /translation="FGRFRMESKSPKLSPEMLVPRLGEYLVQKGLISEIDLQRALNHQ
                     QDVTYKGGQSLLGQALIELKLIDKDTLDQVITEQIIQLRSALQSANRNLEQRVRERTS
                     DLNEALNRLSELSQMKANFVSNISHELRTPLTHIKGYIELMLAESLGPISDEQRHALT
                     VSQRSTGKLEDMIEDLIMFSLASRGEMSMKLEGVDIRRLVSLVTKTAMRKAEDRGVKL
                     EVLVKDDTPYVQADGEKISWVVGQLIDNGIKFTPSGGSVIVAIEEEGKNLVQVIVRDT
                     GVGIPQDRMKEIFEPFHQLDGSSTRRYGGTGLGLSLVRQIVEGHGSLLDVKSTEGKGT
                     TFKFPLLAMRD"
     gene            1043..2230
                     /locus_tag="PKI33_08580"
     CDS             1043..2230
                     /locus_tag="PKI33_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNM37105.1"
                     /translation="MDKETLSDLHRIFQDVQAKPDWRSALDALFISMRRSFVFDNVAV
                     YLSERQTRGLDVVYARAIGRGKTAEADAAWGESVANDVITNEHMVIHGPAKGSNHRDR
                     MSQTFVLGIPLYVGEKLRGALLFVRFGGPEYSDIHIHVASLQAIWAAALIERKELQEA
                     REELDSVQRQMRLQDDFVSTISHELRTPLGFIKGYSTSLLRQDTQWDDATQREFLSII
                     DEETDRLAKLIEDMLESARLQSKTIQFKFSPIRLDAVVRDVAARVKTHHPNLVIEMNM
                     DVLPPVLGDGVRLSQVFENLFSNAIKYAPGSKLIVGGSHQKKKVRISFADEGDGIPEE
                     FLPFLFERFYRVPGERTVTGTGLGLYICKQIVMAHHGNIWVESVLDRGTTFFIELPAD
                     PTL"
     gene            2266..>2823
                     /locus_tag="PKI33_08585"
     CDS             2266..>2823
                     /locus_tag="PKI33_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37106.1"
                     /translation="MAKRILIVDDEPRYLRLLEANLRTEGYEVVTAQDGLQALDVFSS
                     NPIDLVLMDVMMPRLDGFGATQRLREFTNVPIVILTARGEEQDRVRGLDLGADDYLIK
                     PFSATELLARVRAVLRRAHNPTDAGQVRFFTHDDLKIDFARAEVWRGEEAVSLSATEY
                     RLLLQFAHNVGKILTSEELLTSVWGV"
BASE COUNT          709 a          609 c          751 g          754 t
ORIGIN      
        1 attcggacgg tttcggatgg aatcaaagtc gccaaaatta tcaccggaga tgctcgttcc
       61 gcgtttgggg gagtatctcg tccaaaaagg gttgatcagc gaaatagacc ttcaacgtgc
      121 gttgaaccat cagcaggatg taacctataa aggcgggcaa tctcttttgg gtcaggcatt
      181 gatcgaactc aaactcatcg ataaagacac actggatcag gtcattacgg agcagatcat
      241 tcaattgcgc tctgccctac aatcggcaaa ccgcaacttg gagcagcgcg tccgtgagcg
      301 cacctcagac ctgaatgagg cattaaatcg cctgtctgag cttagccaga tgaaggcaaa
      361 ttttgtttca aacatctcgc atgaattaag aactccgctt acgcacatca aaggctatat
      421 tgaattgatg ttggcagagt cgctgggacc catctccgat gaacagcgcc atgcattgac
      481 ggtcagtcag cgttctacgg ggaaactgga agacatgatc gaagatctca tcatgttctc
      541 tttggcatct cgtggcgaaa tgagtatgaa acttgaaggc gtggatattc gccggctagt
      601 ttcattggtt acaaagaccg ccatgcgcaa agcagaagac cgtggtgtga agttggaagt
      661 attggttaag gatgatacgc catatgttca agcagatggc gagaagatca gctgggtagt
      721 agggcaatta atcgataacg gaatcaagtt caccccgtcg ggcggaagcg taatagtcgc
      781 tattgaagaa gaaggtaaaa atctagttca ggtcattgtt cgcgatacag gggtcggtat
      841 tccacaggac cgtatgaaag agatcttcga gccgttccat caattggatg gctcttcgac
      901 gcgacgctac ggcggcacag gattggggtt atcactggta cgtcaaattg tggaagggca
      961 tggttctttg ctggacgtga aatcaacgga gggcaagggg acgacgttta aattccctct
     1021 gcttgccatg cgagattaac ctatggataa agagacgctt tctgaccttc atcgaatctt
     1081 tcaagatgtt caagcaaaac cagactggcg ctcagcactg gacgcgttgt ttatctccat
     1141 gcgtcgttct tttgtgtttg ataatgttgc cgtctattta agtgaacgcc aaacaagagg
     1201 gttagatgtt gtgtatgcgc gtgccattgg gcgtggtaaa accgcagaag cagacgccgc
     1261 ttggggcgaa agcgttgcaa atgacgttat cacgaatgag catatggtca ttcatggacc
     1321 cgcaaaaggt tcgaaccatc gtgaccgcat gtctcaaacc tttgtgttgg ggattccttt
     1381 atatgtgggc gaaaaattaa gaggcgctct tttgtttgtg cgctttggtg gacctgaata
     1441 ttctgatatc catattcatg tcgcttcgct acaggcgatt tgggcggcgg ctttaattga
     1501 gcgaaaagaa ttgcaagagg cgcgcgaaga actggactct gttcagcgcc agatgcgttt
     1561 gcaagatgat tttgtctcta cgatttcgca cgagttacgt actccgcttg ggtttattaa
     1621 ggggtatagc actagcttgt tacggcaaga cacccaatgg gacgacgcca ctcaacgtga
     1681 gttcttgtcg atcattgatg aagagacgga ccgtctggca aaactgatcg aagatatgct
     1741 tgagtccgct cgcttgcaga gtaagacgat ccaatttaag ttttctccta ttcgcttgga
     1801 tgctgttgtc cgcgatgtgg cagcgcgagt caagacgcac cacccgaatt tagtgattga
     1861 aatgaacatg gatgtcctgc cgccagtcct tggcgatgga gtacgtcttt cgcaggtgtt
     1921 tgagaatctt tttagtaatg ccattaagta tgcgcccggc tcaaagctca ttgttggtgg
     1981 aagccatcaa aagaagaagg tgcggatctc atttgctgat gaaggcgatg ggattccaga
     2041 ggaattccta ccattcttgt ttgagcgatt ttatcgtgtg cccggtgaac gcacggtgac
     2101 cgggacgggt ttagggctgt atatttgcaa gcaaattgtt atggcacatc atggtaatat
     2161 ttgggtagag tcagtgttgg atcgaggaac tacatttttc attgagcttc ctgccgaccc
     2221 aacgctttga catttaagtg aaacacgatt cttcaaggag ttcttatggc aaagcgtatt
     2281 ctgatcgtag atgatgaacc ccgctatctt cgtctgttag aggcaaacct tcgtacagaa
     2341 ggatatgagg tagtaaccgc ccaagacggc ttacaagcgc tggatgtctt ttcatccaac
     2401 cccatcgacc ttgtgttgat ggatgtgatg atgcccaggc tggatggctt tggtgcaacc
     2461 cagcgtttgc gtgaattcac caatgtgcct attgttattc tgacggcacg cggcgaagaa
     2521 caagatcgtg ttcgtgggct agaccttgga gcagatgatt acctgatcaa gcctttctct
     2581 gcaactgagt tgcttgctcg tgttcgtgct gtgctacgcc gtgctcataa cccaaccgat
     2641 gcagggcaag tgcgcttctt tacacatgat gacctgaaga tagactttgc ccgtgccgaa
     2701 gtgtggcgtg gggaagaggc agtttctctc tctgctacag aatatagact actgcttcaa
     2761 tttgctcaca acgttggcaa gattctcact tctgaagaat tgctgaccag tgtttggggt
     2821 gtt
//
LOCUS       DAOREF010000374         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24636_length_2811_cov_4.772134, whole genome shotgun sequence.
ACCESSION   DAOREF010000374 DAOREF010000000
VERSION     DAOREF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24636_length_2811_cov_4.772134"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..524
                     /locus_tag="PKI33_08590"
     CDS             75..524
                     /locus_tag="PKI33_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37107.1"
                     /translation="MSDKLPDLAERLKSKPAHYSYMKHRNQVMRQIILPVALSALVLV
                     GIVVWVSYATFNQGGDVGRWAAISTIWVVIPMLLGGLVVLAILGGLIYGMARLLGALP
                     YYTGIAQDYIFIARGYIIRGANMVVKPINALNDWLQTAKAFFERMTP"
     gene            521..712
                     /locus_tag="PKI33_08595"
     CDS             521..712
                     /locus_tag="PKI33_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37108.1"
                     /translation="MKNTRLLLGGALIGAIIGLVAALLIGRRAEKNESETAITASEGV
                     KLGVLVFGLLRAISSLGDD"
     gene            794..2056
                     /locus_tag="PKI33_08600"
     CDS             794..2056
                     /locus_tag="PKI33_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNM37109.1"
                     /translation="MDTISKQTYRRFLLGSQGLWPGRRFSGLSGTESALRQMQALQLD
                     PLVMVARSQDIAMYGRVLDYKPAMLYKMAYEKRKAFDYGGWLMMYPMQEFPYWQLHMQ
                     RRKEQGLRWEHFTHPPKQVVKFVLDELREKGPLGNRDIEGDAVKAWSYRGRKETSITL
                     FYLWLVGEVMISNRKGFDRLYDLRERVLPAEYDYAAPTEEAEDFFAKKHIAFSGIARE
                     PSWKTGVGDFIHRKVTAKEGATWIERLIEKGDIARVGVEGFRENFLVLSSDQAHLSAL
                     EAGRIPKAWKHKGPSTLEEVTFLAPLEIVSARGRAKKVFDFDYVWEVYKPLHQRKWGY
                     YTLPILYGDDLVARMDPKHDRTTNTLQILGFWLEEDAPRDADFANALAKGLKRFAEMI
                     GATKIDLVGVKPVKLKSELKMLLKRIGF"
     gene            2091..2543
                     /locus_tag="PKI33_08605"
     CDS             2091..2543
                     /locus_tag="PKI33_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM37110.1"
                     /translation="MNYSLRSATIQDAPAVTACVNHAFGHYVERIGMKPAPMEMDYEH
                     EIREHQVFVVEDAGQVVGSLVLGITQEGFLLDVIAVEPKYWGKGVGRIMLEHAEAEAK
                     QQGFDSIYLFTHEKMVENQALYKKVGYVEYDRRLEMGRNRVYMRKQLV"
BASE COUNT          723 a          619 c          699 g          770 t
ORIGIN      
        1 cagataatca ctaaacaagc aaagacccta aaggttcaaa cctttagggt cttttatttt
       61 ggtaaaatat ctttatgagc gataaactcc ccgatctcgc tgaacgtcta aaaagcaaac
      121 ccgcccatta ctcttacatg aagcaccgta atcaggtaat gaggcaaatc atcttgccag
      181 tggcattgag tgccttggta ttggtcggaa ttgtggtgtg ggtgagttat gccaccttca
      241 accaaggtgg tgatgtcggg cgctgggctg ccatctccac aatttgggtc gtgatcccca
      301 tgttgctcgg cgggttggtg gtacttgcca tccttggcgg cttgatctat ggcatggctc
      361 gtttacttgg tgcgttgcca tattacaccg gcattgccca agattatatt tttatcgcac
      421 gcggctatat cattcgcgga gcgaatatgg tcgtcaaacc cattaacgct ctgaacgatt
      481 ggcttcaaac tgcaaaagcc ttctttgaaa ggatgactcc atgaaaaata caagactact
      541 tttaggcggc gcactcatcg gcgctattat cgggctggtt gccgctttgc tcatcggtcg
      601 ccgcgccgaa aagaacgaga gcgaaactgc catcactgca agtgaaggcg tcaaattggg
      661 cgtgctggta tttggcttgc ttcgcgcaat ctcttccctt ggcgacgatt aacagacgat
      721 aaacgattga ccacagaccg aggttcctgc ctcggtcttt tttatcttga cctttgactg
      781 tagactctta accatggata ctatctccaa gcaaacatac cgccgctttc ttctcggctc
      841 acagggactt tggcctgggc gccgtttctc gggcctttct ggcaccgaat ccgctttgcg
      901 ccagatgcag gcgttgcaac ttgacccact tgtgatggtt gctcgtagtc aagacattgc
      961 catgtatgga cgtgtcctcg attacaaacc cgctatgcta tacaaaatgg catatgaaaa
     1021 acgaaaagcc ttcgattacg gcggctggtt gatgatgtac cccatgcagg agtttccata
     1081 ctggcaactg cacatgcagc gacgtaaaga acagggtttg cgctgggagc atttcacgca
     1141 cccgcctaaa caagtcgtta agttcgtgtt ggacgagctg cgcgaaaagg gtccgcttgg
     1201 gaatcgtgat attgaaggcg atgccgtcaa agcatggagc taccgtgggc gtaaggaaac
     1261 atccatcact ttgttttatc tctggctggt gggtgaggtg atgatttcca accgcaaagg
     1321 ttttgaccgt ctctacgatc tgcgcgaacg cgtcttgcca gcggaatatg attatgccgc
     1381 tcctactgaa gaagccgaag atttctttgc aaaaaagcat attgctttct caggtattgc
     1441 gcgcgaacct tcatggaaga ccggcgtcgg tgatttcatc catcgtaaag tcaccgcaaa
     1501 agagggtgcc acatggatcg aacgtctcat tgaaaaaggc gacatagctc gagtaggagt
     1561 ggaaggcttc agagaaaact tccttgtgtt gagcagcgac caagcccact tgtctgctct
     1621 tgaggcgggg cgcatcccca aggcgtggaa gcataaaggt ccatccacat tggaagaggt
     1681 cacattcctg gctccgttgg aaattgtcag cgctcgcggg cgcgccaaga aagtttttga
     1741 ctttgattat gtgtgggaag tttataagcc tttgcatcaa cgcaagtggg gctattacac
     1801 attgccgatt ttgtatggag atgacttggt tgcacgtatg gacccaaagc atgaccgtac
     1861 cacaaatacc ttgcagatcc ttggcttttg gttggaagaa gatgccccaa gagatgctga
     1921 ttttgcgaac gcccttgcaa agggactgaa gcgttttgct gagatgatcg gcgctacaaa
     1981 gatcgatttg gttggtgtaa aacctgttaa gctcaaatca gaattaaaga tgcttctcaa
     2041 gcggataggt ttttgacctt cttagtaacc tatctttagg aaaatgatac atgaattact
     2101 ccttacgctc cgctacgatt caagatgctc ccgctgttac ggcttgtgtg aatcatgcct
     2161 ttggtcatta cgtggaacgg attggcatga agcctgctcc tatggagatg gattacgaac
     2221 atgagattcg tgagcatcag gtctttgttg ttgaagatgc tgggcaggtt gttggctcac
     2281 ttgttttggg aattacccaa gaaggctttt tgctagatgt gatcgcagta gagccaaaat
     2341 attggggtaa aggtgttggg cgcatcatgc tggagcatgc tgaagcagaa gccaagcaac
     2401 agggttttga tagtatctat ctctttactc atgagaagat ggttgagaat caagctctgt
     2461 ataaaaaggt cggatatgtt gaatatgacc gtcgccttga aatgggacgt aaccgagtat
     2521 atatgcgcaa acaacttgtt tagactaaac ggtttttgaa aatagacctc tcactcggat
     2581 tattcagcca atttggtcta attgcttgac ttcaacggca agacaattta tattaacaag
     2641 gcattgggac ttttcattca ttgaaattat ttttattctg agccatcctg tttaaatctc
     2701 ccaatgtgaa aatttaaatc atcgaattcg caagacctaa gcaaggtttt tacaaccttt
     2761 gtatgtagaa aaggcaaaaa gaattcgaga ggatgccaag atggtttcag g
//
LOCUS       DAOREF010000375         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24638_length_2811_cov_3.632801, whole genome shotgun sequence.
ACCESSION   DAOREF010000375 DAOREF010000000
VERSION     DAOREF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24638_length_2811_cov_3.632801"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKI33_08610"
     CDS             complement(<1..504)
                     /locus_tag="PKI33_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM37111.1"
                     /translation="MQFPQFDLSGSGAPIHFLHANGYPPECYQPLLELLNQKHRVFGM
                     KLRPLWDGAKPEDLDDWHPLSDDLLRFLSDREPDPVIGIGHSIGGIVTLRAAIREPEK
                     FRALILLDPVLFLPSFLIGWNVLRAIGLGEKTHPLIAGTKKRRRTFDDLETIFRGYRT
                     RPIFRYLS"
     gene            complement(533..1435)
                     /locus_tag="PKI33_08615"
     CDS             complement(533..1435)
                     /locus_tag="PKI33_08615"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNM37112.1"
                     /translation="MSKLIPLKKAIAEYVSDGDVVYAAGFTHLIPFAAGHEIIRQGKK
                     NLTLARATPDLIYDQLVAAGCAKKVIFSYMGNPGVGSLRIVRGAIERGELEWEEYSHF
                     GMITRLQAGASGLPFLPMNQTGAEDLEKVNPQIKRIPDPYGGKDVIVVPALNPDVAIV
                     HVQRADKNGNAHLWGIIGEQKEAAFAAKKVILTAEEIVDESVIRSDPNRTMIPGIVVS
                     AVCHVPFASHPSYSQGYYDRDNEFYLAWDKISESTESVKKYLDEWVYGVKDRNAYWKM
                     LGEKAQKRLKIKARYSEKINYGSY"
     gene            complement(1450..1731)
                     /locus_tag="PKI33_08620"
     CDS             complement(1450..1731)
                     /locus_tag="PKI33_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37113.1"
                     /translation="MDTEDPFEVRVGTFFVVMGMGAFLLFVISDLTDKVDFDFFFVAL
                     ILLVIGYYLRRTKAPPPPSGRFEWFKGFWGNTRWGRKGGGGGEGKDKKG"
     gene            complement(1823..2809)
                     /gene="fni"
                     /locus_tag="PKI33_08625"
     CDS             complement(1823..2809)
                     /gene="fni"
                     /locus_tag="PKI33_08625"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNM37114.1"
                     /translation="MKINLEQDVRSALTTGLEDYHFTHEALPELDLNRLNTSLSLFGK
                     TLTSPTLVSSMTGGTDEAETINLRLAEAAQECGVAMGVGSQRAAIEHPEQAKTFQVRR
                     VAPDILLFANLGAVQFNYGYGIDQCCRAVDMIQADALYLHINPLQEAVQDAGDTNWVG
                     ITKKIEEVCKKLEVPVIAKEVGWGFSEKTAKVLADCGVSAIDVAGAGGTSWSQVEMYR
                     APDEFTRRLAATFVGWGIPTAEAIQNVKRAAPDMTIFASGGLKDGLDIAKSIALGATL
                     GGMAGEFLKAAAISTERAIEMMKLTKRQIEVTMFTSGAGTLHELAEKLTKKA"
BASE COUNT          676 a          674 c          714 g          747 t
ORIGIN      
        1 acttaagtag cgaaaaatgg gacgcgtgcg atatccgcga aagatagtct caaggtcatc
       61 aaaggtgcgg cggcgttttt ttgtccctgc gatgagtgga tgggttttct ctccaagccc
      121 aattgcacgc aagacattcc atccgattaa aaatgatggg agaaaaagca cggggtctag
      181 caggatgagg gcgcgaaatt tctctggctc gcgaatggcc gcccgcaaag tgacaatgcc
      241 tccgatggaa tgaccaattc ctataacagg atcaggctca cggtcagaaa ggaagcgcag
      301 caagtcatca gagagtggat gccagtcatc caaatcttca ggcttggctc catcccaaag
      361 tgggcgcagt ttcatcccaa agacgcgatg cttttgattt aataactcca aaagcggctg
      421 ataacattca ggtggatatc cattggcgtg aaggaagtga atcggcgctc ccgaaccgct
      481 gagatcaaat tgtggaaatt gcatgttaga tttgttcgcg tggattagcg gatcaataac
      541 ttccgtaatt gatcttttcg ctatagcggg cttttatctt cagtcgtttc tgagcttttt
      601 cgccgagcat cttccagtac gcattgcggt cttttacacc gtagacccat tcatcgagat
      661 attttttaac gctttctgtt gactcgctga tcttatccca tgcgagatag aactcattat
      721 ctcgatcgta atatccctgt gaataggatg gatgactcgc gaatggaacg tgacatactg
      781 cactgacgac aatgccggga atcatcgtgc ggttcgggtc ggacctgatg actgattcgt
      841 cgacaatttc ttcagcggta aggatgactt ttttcgcggc gaaagcggct tctttctgtt
      901 cgccgatgat gccccaaaga tgggcattgc cattcttatc tgcgcgctgt acgtggacaa
      961 tggcaacatc tggattcaag gctggcacga caatcacatc tttgccgccg tatgggtcgg
     1021 ggatgcgctt gatctgcgga ttaacttttt ccaagtcttc tgcaccggtt tgattcatgg
     1081 gcaggaatgg caagccagat gcacctgcct gtaagcgagt gatcatgccg aagtgagaat
     1141 attcttccca ttccaactct ccacgctcaa tggcgccacg cacgatgcgt aacgaaccaa
     1201 cgccgggatt tcccatatag gagaaaatca ctttcttggc acaacccgcc gcaaccagtt
     1261 gatcgtagat caggtctgga gtggcacgag caagcgtgag attctttttg ccctgacgaa
     1321 tgatctcatg cccggcagcg aatgggatga ggtgtgtgaa cccggcagcg tagaccacgt
     1381 cgccatcact aacgtattct gcaattgctt ttttgagtgg aatcagtttt gacataaaga
     1441 aagtatgaat tatccttttt tatcttttcc ttcaccgcca ccgccgcctt ttctacccca
     1501 gcgtgtgttt ccccaaaagc ctttgaacca ttcaaaacgc ccagaagggg gaggtggggc
     1561 ttttgtgcgg cgaagataat aaccaatgac gagcaagatc agggcaacga agaagaaatc
     1621 aaaatcaact ttgtcggtca aatcggagat tacaaatagg aggaatgctc ccattcccat
     1681 gactacaaag aatgtgccca cgcgaacttc aaatggatct tcagtatcca tactcgtatt
     1741 ttaccgcaag ggaaaaagca gagaatcgtg atttatatcc cccattggaa ttacaaaaat
     1801 agcggcgaaa acgccgctat ttttatgctt tttttgtgag cttttctgca agttcgtgta
     1861 acgtccctgc gcctgaagtg aacatggtta cttcgatctg gcgttttgta agcttcatca
     1921 tttctatggc tcgttcggta gaaatagcag cggctttcag aaattcacct gccatgccac
     1981 caagggttgc accaagggcg atagatttgg cgatatctag tccgtctttg agcccgccac
     2041 tggcgaagat ggtcatatcg ggggcggctc gtttgacgtt ctgaatcgct tccgctgttg
     2101 gaattcccca gccgacaaaa gtagctgcca gtctgcgtgt gaattcatca ggggcacggt
     2161 acatttctac ttgagaccaa ctcgtgccgc ctgcaccagc aacgtcaatg gcggagacgc
     2221 cacaatcggc taagaccttg gctgtttttt cagagaagcc ccagccaact tctttggcaa
     2281 tgacggggac ttcaagtttt ttacatactt cctcgatttt ttttgtaatg ccgacccagt
     2341 tcgtatcccc tgcatcttgc acggcttctt gtaacgggtt gatgtggaga tacaacgcat
     2401 cagcttggat catgtccact gcgcggcagc attggtcgat gccgtagccg tagttgaatt
     2461 gcacggcacc aagattggcg aataataaaa tatctggcgc tactctgcgg acttgaaatg
     2521 ttttggcttg ttcggggtgc tcgatggcgg cgcgttgact ccccacgccc atggcaactc
     2581 cgcattcttg ggcggcttca gcgaggcgca aattgatggt ttcggcttcg tctgtgccgc
     2641 cggtcatgga acttaccagt gtaggggagg ttagggtttt gccgaacagg ctcaggcttg
     2701 tgttgaggcg gtttaagtcc aattcaggca gggcttcgtg ggtgaagtga tagtcttcca
     2761 gaccagttgt cagcgcagag cggacgtcct gttctaagtt gattttaatg t
//
LOCUS       DAOREF010000376         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24643_length_2811_cov_2.853774, whole genome shotgun sequence.
ACCESSION   DAOREF010000376 DAOREF010000000
VERSION     DAOREF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24643_length_2811_cov_2.853774"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKI33_08630"
     CDS             complement(<1..554)
                     /locus_tag="PKI33_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HNM37115.1"
                     /translation="MCGIVGYIGPREATPIILNGLKRLEYRGYDSAGVAVINNNQIDM
                     RRDAGKLQQLVDLVAKSPLVGAPGIGHTRWATHGAPSARNAHPHIGNTGKMVVVHNGI
                     VENFLELKDELTAEGVTFNSDTDTETIVHLAEHHKAAGISFEDAARRTFKQIEGANVV
                     LLLSTDEPDKIIAARIGNAGGVVVG"
     gene            complement(976..2544)
                     /locus_tag="PKI33_08635"
     CDS             complement(976..2544)
                     /locus_tag="PKI33_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNM37116.1"
                     /translation="MGLILSDRKNKKTKSKAQAMVEFALVIPILMLLVVGIIEYSRLL
                     FAWIIIENSTRFGIRYATTGQYDQNYCNGGGIYNVSTYDLDADGDLCSTDPEIDTAPR
                     VFSIYDETRKIVVGFNLKDVTRGSTATTSDDEYFRITVCSAEDGRVFTPPLMARPVYA
                     DCRLPNNTHSEHAGTPGNRVVVAADYNFRFIVLPIFGFAPTIHLASYREGIVEQFRAT
                     RAINTPLPLNIPTVPSPTLTFTLSPTITLTPTPTLTRTPTRTPTITPSPTITRTPTST
                     PTTTNTPAPSCSNIFINRTRFNGDRFEARVINNNFATAYLISSTLSWNPSTLTGGKYF
                     DFVAFNGSTYYNPAGTSGPGLTVSPLTTNANSGDLPLAGNGTQVTWDADTNNSTWAGA
                     WDVSLTFDFPGWGTCTVNSSINNYTATPTNSFTPTMTPTITRTPTITRTPTITLTPTI
                     TRTPTITFTPSKTPTITLTPTKTNTPTITPTPTRTNTPTQTPTRTPTVITPTPTRTPT
                     PSKTPTTTPCFEGC"
     gene            complement(2569..>2811)
                     /locus_tag="PKI33_08640"
     CDS             complement(2569..>2811)
                     /locus_tag="PKI33_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37117.1"
                     /translation="YLADPVNGQGVTVFTIGLGYQIRNQVKGDANAGEALLQYAATTA
                     GGTSANHGEYFYSPDSSGLATIFSRIADNIFTRISR"
BASE COUNT          729 a          598 c          691 g          793 t
ORIGIN      
        1 cccactacca caccaccagc attcccaata cgggcagcga tgatcttatc tggctcatcg
       61 gtggaaagca gcaagactac gtttgcgcct tcgatttgct taaatgtacg gcgagcagca
      121 tcttcgaaag aaataccagc cgctttatga tgttctgcca agtgaacgat tgtctcagtg
      181 tctgtatctg aattgaacgt taccccttcg gcggtcagtt catctttgag ttccaaaaag
      241 ttttcaacga tcccgttgtg taccaccacc atcttgccgg tgttgccaat gtgggggtga
      301 gcgttacgag ccgatggtgc cccatgcgtt gcccaacgtg tatgcccaat ccctggcgca
      361 cccaccaatg gcgatttggc aaccaaatcc accaactgtt gcagcttgcc cgcatctctg
      421 cgcatatcga tctgattgtt attgatgaca gccacgcccg cagagtcata tccgcgatat
      481 tcaaggcgct ttaagccgtt aagaattatc ggtgtcgctt cacgcggacc gatataaccg
      541 acgattccac acatagtagg aaacctccaa attttgattg aattgagaac tgaaaaccag
      601 gcgcctcaca acaaggcgac gcctgctctt acagcccgat atctttgcca cgcccaagcc
      661 ttttgatccg tgatcgctca cggggttgta acctaggttt catctctaga gggaaagatg
      721 ggcgggtcat ggcagacccg gaaggcttcc gctgatctct cgattttcca actcaaatta
      781 ttttgagccg ttagctccat tttgcaatgg agaaccttca tcctcgtcaa ctctggataa
      841 gaatacacca gagcccatgc gctgtctttc cctttttatt ttagtattgc acctcctttc
      901 atagtttttt agattatacc tgaaaacctt ttttctcaac aaagccccca tccgtaggat
      961 gggggctttg tacttctaac aaccttcaaa acatggggtt gtggttggcg tcttggacgg
     1021 tgttggtgtc cttgttggcg ttggcgtgat taccgttggt gttctggtag gtgtttgggt
     1081 tggcgtattt gtcctagttg gtgtaggtgt aatggttggg gtattcgtct ttgtcggggt
     1141 aagtgtaatg gtcggcgttt tagacggcgt gaatgtaatg gtgggtgtgc gcgtgatggt
     1201 tggggtaagc gtaatggttg gcgtgcgcgt aatagtgggt gtacgcgtaa tagtgggtgt
     1261 cattgttggg gtaaatgaat ttgttggagt tgcagtgtag ttattgatgc tagaatttac
     1321 tgtacaagtg ccccaaccag ggaaatcaaa tgtcaagctg acgtcccagg cgccagccca
     1381 tgtggaatta ttggtatctg catcccaggt tacttgagta ccattgccag cgagtggtaa
     1441 atcacctgaa ttcgcatttg tagttaacgg agaaaccgtt aatccaggac cagaagttcc
     1501 cgcgggattg tagtaagtgg atccgttaaa agctacgaaa tcaaaatatt ttccaccggt
     1561 caatgtggat gggttccaac tcaatgtgga actaatgagg tatgcagttg caaagttgtt
     1621 gttgataaca cgtgcctcaa atctatcacc attgaaacgc gtacgattaa taaaaatgtt
     1681 tgaacacgaa ggtgcaggtg tattcgttgt tgtaggggtg ctggtggggg tcctagtaat
     1741 tgttggcgat ggagtaattg taggggtgcg agtaggtgtg cgagtaagcg taggggtagg
     1801 cgttaacgtt atagtagggg aaagtgtaaa tgttaaggta ggggatggaa ctgtggggat
     1861 gtttaacggt aggggggtat taatagcacg agttgcacgg aattgttcta caataccttc
     1921 acgatatgag gcaagatgaa ttgtaggtgc aaacccaaaa attgggagaa ctataaacct
     1981 aaaattgtaa tcggcagcaa caactacacg attgccaggg gttccagcat gttcactgtg
     2041 ggtattattg ggaaggcggc aatcggcgta aacagggcgc gccattaatg gcggagtaaa
     2101 gacacgccca tcttctgcag aacagaccgt aattctgaaa tattcatcat cactagttgt
     2161 tgctgtagac ccacgagtaa cgtcttttaa gttaaaccca actacaattt ttcgcgtttc
     2221 atcatagata gaaaatacac gcggagcagt atctatttcc ggatccgttg aacacaagtc
     2281 tccatcagca tctagatcgt aagtagaaac gttgtaaata cctccaccat tgcaataatt
     2341 ttgatcgtat tgacctgttg tggcatagcg aatgccaaag cgagtggaat tttcaataat
     2401 aatccacgca aataacaagc gtgagtactc aataatccca acgacgagga gcatcaaaat
     2461 gggaatcacc aaagcaaatt caaccattgc ctgtgccttg gattttgttt ttttattttt
     2521 tcgatcagaa aggataagtc ccatagctag ttcctcgtta tttaataact atcttgaaat
     2581 tctggtaaag atattatctg ctattctact aaaaattgtt gccaaaccgc tcgagtctgg
     2641 tgaataaaaa tattctccat gattggcaga agtcccaccc gcagtagttg ctgcatattg
     2701 caatagagcc tctccggcat tcgcatcacc ttttacttga tttcggattt gatagcctaa
     2761 gccaatggtg aacacagtta ccccttgccc attcacaggg tcggcaaggt a
//
LOCUS       DAOREF010000377         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24715_length_2806_cov_5.513631, whole genome shotgun sequence.
ACCESSION   DAOREF010000377 DAOREF010000000
VERSION     DAOREF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24715_length_2806_cov_5.513631"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKI33_08645"
     CDS             <1..389
                     /locus_tag="PKI33_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM37118.1"
                     /translation="KLDVASQKVTRALRSAEETISLEGPVGDDESSALGDFIEDEDAL
                     SPMDAAAREMLREQITHALESLSDRERDVLELRFGLKDGKEHTLEEVSRFFDVTRERV
                     RQIEAKALRKLRHPSKSRELRDYLGD"
     gene            591..947
                     /gene="ndhC"
                     /locus_tag="PKI33_08650"
     CDS             591..947
                     /gene="ndhC"
                     /locus_tag="PKI33_08650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNM37119.1"
                     /translation="MLLEYIAIGVMILVATLIAFIAIGLGELFGPKKKSAGKDTPYES
                     GMNPYGEGTRRMPVRFYLIAVLFILFDIEVVFFLPWAIAFRKLGLFGLIEMVVFITVL
                     LIGYVYAWKKGALEWE"
     gene            938..1432
                     /locus_tag="PKI33_08655"
     CDS             938..1432
                     /locus_tag="PKI33_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNM37120.1"
                     /translation="MGVEQKLGNMGVVTTTLENVVNWSRTNAMWPMLFGLACCAIEMM
                     AAQAAHYDMSRFGMELMRASPRQSDLMIVAGRVSKKMGPVLRRLYDQMPEPKWVIAMG
                     DCASCGGVFNNYAIYQGVDEVVPVDVYVAGCPPRPEALIHGVMTLYEKVNAMKLAEKP
                     AESK"
     gene            1483..1995
                     /locus_tag="PKI33_08660"
     CDS             1483..1995
                     /locus_tag="PKI33_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNM37121.1"
                     /translation="MEKNLEPIVKDLQTKFGATYEEFRGEVQCMVKAENIVDVLKTLR
                     DEHSFELLSVLNAIDYYPQTSPRFHVVYQLNSLSKNLRVQVRVPVNDDQPKVPTVTGV
                     FKTGMWREREVFDMFGIEFEGHPDPRRILMPEGTEGHPLRRDFPLGYEEPQFTFNLEE
                     IDLRKPYAKE"
     gene            1999..>2806
                     /locus_tag="PKI33_08665"
     CDS             1999..>2806
                     /locus_tag="PKI33_08665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNM37122.1"
                     /translation="MTQLQEVSVEQFKHLVSERALTGETMLLNMGPQHPSTHGVLRLL
                     LELDGETVVNCIPDIGYLHTGIEKNMEAKTYQKAEVMTDRLDYMNPMGNNLSYVMAVE
                     KLVDLDVPPRAQALRVILTELQRISSHLVLIGTWGLDLAAMSMFLYCFREREQILDIF
                     ELVSGQRMMTTFFRPGGLWRDVPVEFEKAVRDFLKIFPKRIDEYEKLLTKNPLFVDRM
                     VDIGYLSKETALSYGVTGPNLRASGVNWDLRKARPYMGYEQYDFDVPVLTE"
BASE COUNT          701 a          651 c          709 g          745 t
ORIGIN      
        1 gcaagttaga tgtcgcttcg caaaaggtca ctcgtgcttt gcgctctgcc gaagaaacca
       61 tctcgcttga aggtccggtg ggggatgacg aatccagcgc tttgggcgat ttcattgaag
      121 atgaagacgc gctttcgccc atggacgctg ccgcccgcga aatgctgcgt gagcaaatta
      181 cgcacgcctt ggaatctctt tcagaccgtg agcgcgacgt cttggaattg cgttttggac
      241 ttaaagatgg aaaagaacat acacttgaag aagtcagccg cttctttgat gtgacccgtg
      301 aacgggtgcg ccaaattgaa gccaaagcac tccgcaaact tcggcaccca agcaagagcc
      361 gagaattaag agattattta ggtgattaat aaaccaaagt gacagtttcc ataacgagac
      421 tgtcactttt ttgttttctt ggggtgtttt ctatgaattc gattacattt tgcccaacat
      481 gacaaaaatc atcatttttg tgtaatgtag cacaagcgct gtgcttaaaa aaacctttgt
      541 gatatacttt cgcgaactgt gcaagatttc acacatccat agaggtgttt atgctgctgg
      601 aatatattgc cattggggtc atgatcttag tggccacgtt gatcgctttt attgcgatcg
      661 gtctaggtga gctttttggt ccaaaaaaga aatctgcggg gaaagatacg ccctatgaat
      721 cgggcatgaa tccatatggt gaaggaacgc gtcgtatgcc ggtgcgtttc tatttaatcg
      781 ccgtattatt cattctgttt gatattgaag ttgtattctt tttaccttgg gcgatcgcat
      841 tccgcaagct cggtctcttc ggcttgatcg agatggtggt tttcattacg gtccttctta
      901 ttggttacgt ctatgcttgg aaaaaaggag ctttggaatg ggagtagaac aaaaactcgg
      961 aaatatgggc gtggtaacca ccacgcttga aaatgttgtg aactggtcac gcaccaacgc
     1021 catgtggccc atgttgttcg gtttggcttg ctgtgccatt gagatgatgg ctgctcaagc
     1081 cgcccactat gatatgagcc gcttcggtat ggaactcatg cgtgccagcc ctcgccagtc
     1141 tgacttgatg atcgtggctg ggcgtgtttc caagaaaatg ggacccgtcc ttcgccgttt
     1201 gtatgaccag atgcccgaac ccaagtgggt gatcgccatg ggcgattgcg cttcttgcgg
     1261 tggcgtcttc aataactatg ccatctatca aggtgtggat gaagttgtgc ccgtggacgt
     1321 gtatgtggct ggctgccctc cgcgccctga agccttgatc cacggcgtga tgaccttgta
     1381 tgaaaaagtc aacgcgatga agctggcaga aaaacccgca gaatcgaaat aaaaaaaaca
     1441 gacctgacag atttcaagac ctgtcaggtc ttaggtgtat ttatggaaaa aaacctcgaa
     1501 cctattgtaa aagatctgca gacgaaattc ggcgcaacgt acgaagagtt ccgtggtgaa
     1561 gtgcaatgta tggtgaaagc cgagaatatc gttgacgtgt taaagacctt gcgcgacgaa
     1621 cattccttcg aattactctc agtgcttaat gccatcgatt actacccaca aacctcacct
     1681 cgttttcatg ttgtgtacca gctcaattca ctctcaaaaa atttacgggt gcaggtgcga
     1741 gtccctgtca acgatgacca gcccaaagtc ccgaccgtga ccggcgtgtt caagaccggc
     1801 atgtggcgcg aacgtgaagt cttcgatatg ttcggcatcg agttcgaagg tcatccagac
     1861 ccgcgccgca ttctgatgcc cgaaggcacc gagggacacc cgctgcgcag agacttcccg
     1921 cttggctacg aagaaccgca attcactttc aacttggaag agatcgacct tcgcaagcca
     1981 tatgcaaagg aataatttat gacacaacta caagaagtga gtgtagagca atttaaacat
     2041 ctggtttcag agcgcgcgct gaccggcgag accatgctct tgaacatggg tccgcaacat
     2101 ccatccacgc atggtgtgtt gcgtttgttg ttggaactcg acggcgaaac agtggtgaac
     2161 tgtattcctg atatcggcta tttgcatacc ggtattgaaa agaatatgga agccaagacc
     2221 taccaaaaag cggaagtgat gaccgaccgc cttgattata tgaaccccat gggaaataac
     2281 ctctcctatg tgatggctgt cgaaaagttg gtagacctgg atgtgccgcc gcgtgctcaa
     2341 gctttgcgcg ttatccttac agagcttcaa cgtatttcct ctcacctcgt gttgatcggt
     2401 acatggggct tggatctggc agccatgtcc atgttcttgt actgcttccg cgaacgtgaa
     2461 cagatcctcg atatttttga actcgtttct ggtcaacgca tgatgaccac attcttccgt
     2521 cccggcggtt tgtggcgcga tgtgccggtt gaatttgaaa aagccgtgcg cgatttcctc
     2581 aagatcttcc ccaagcgcat tgatgaatat gaaaaacttt tgacgaagaa tcccctgttt
     2641 gtggatcgta tggtggatat tggttatctg agcaaggaaa cggctctttc ctacggggta
     2701 acgggtccga acctgcgagc ctcaggtgtc aactgggacc ttcgtaaagc ccgtccctat
     2761 atgggttatg aacaatatga tttcgatgtg ccggttctaa ccgaag
//
LOCUS       DAOREF010000378         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24798_length_2801_cov_4.332484, whole genome shotgun sequence.
ACCESSION   DAOREF010000378 DAOREF010000000
VERSION     DAOREF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24798_length_2801_cov_4.332484"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..733
                     /locus_tag="PKI33_08670"
     CDS             38..733
                     /locus_tag="PKI33_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37123.1"
                     /translation="MKKALTITIALLLTACGVVQPATQEVLPTPIVQTVVVIATSEAT
                     QPLPPTAISLPTQGPTNTAEPVNTATTAPTETLVPTSAPVATEVVPTLSSSSTTELKP
                     VNVSNDLGKGVFKDITFSSDLLTLQCYPREMTITITANLPEIVSAIMYYRMVDNPDAL
                     YPSEWKNLGEMVSDKQGHFSFVLESLDLNPDLRVLEKAWIDFQFIGINKGGGVVDRTQ
                     KIEKMVTYYKACP"
     gene            complement(775..1032)
                     /locus_tag="PKI33_08675"
     CDS             complement(775..1032)
                     /locus_tag="PKI33_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37124.1"
                     /translation="MIRTIVFKTVASILLMLGALGLSMPMNAYAQSGTQHIQSAQYSG
                     KCEKIKDKKQKKACEKAQKAGKTKKNDDGPNHESNDGANHH"
     gene            complement(1156..2472)
                     /locus_tag="PKI33_08680"
     CDS             complement(1156..2472)
                     /locus_tag="PKI33_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNM37125.1"
                     /translation="MKTIQRFLAMFVLVALFSLTFASPALAFDGRSGDKIVIAADEVI
                     NDDLYVSAEEFTLDGTVKGDLVVFGAYIFINGTVEGDLIAAGNTIVIDGTVKDDARIA
                     GAALQLGEKASIGGDLISAGASLEAQNGSAVGQDVVFAGGQSLLAGSIGRNLWVGTGA
                     LELSGEIGGDVQAEVGEMQEGSPSPSMYIGDVKVSTPSVQPGLTVTESAKIGGDFTYT
                     QSKDIKFPSDAIEGKVTRKEPTVDTQSVPTPPTAGEVAATWVFDLLRKIATLAIFGLL
                     LVWLVPGFLKGLGDKLQSQPIPSFGWGIVAYAAFFFAILLIVIVMIVGGIIFGVISLG
                     GVTGTIIWVGLLSIFAMIVGFALFTGFGAQILVAWLGGKWILGRFNPALAEHKVWPLL
                     LGVVIVGLLVKLPLVGWLIGFLIMFFGLGALWLWGRERMKKPEAVA"
     gene            complement(2604..>2801)
                     /locus_tag="PKI33_08685"
     CDS             complement(2604..>2801)
                     /locus_tag="PKI33_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37126.1"
                     /translation="EISEIYEGRLNGKSVQFDPVEISEVRFVSVDVVKAGLSQSPELY
                     CYWFIQLMNWYFGLPAKLKEL"
BASE COUNT          805 a          707 c          569 g          720 t
ORIGIN      
        1 tgggcataga atggaataaa tttacaaagg agttttcatg aaaaaagcac tcactattac
       61 catagccctg ctcctgacgg catgcggcgt tgtacagcca gccactcagg aagtattgcc
      121 aacgccgatt gtgcaaactg ttgtagttat tgctacatcc gaagctacac aaccgcttcc
      181 gcctactgca atttcacttc ccactcaagg accgacaaac actgctgagc ctgttaatac
      241 tgcgactacc gcccctactg agacattggt acctacttct gccccagtag caactgaggt
      301 tgtaccgact ctttcgtctt catcgactac agaactgaag ccagttaatg taagtaatga
      361 tttgggcaag ggcgtcttca aagatattac cttttcaagc gatcttttga cacttcagtg
      421 ttatccgcgt gaaatgacca tcactattac cgcaaatctt ccagagattg taagtgcaat
      481 tatgtattat cgtatggtcg ataaccctga tgcgctttac ccctctgaat ggaagaacct
      541 tggcgagatg gttagcgaca agcaaggaca tttttcgttt gtcttggaat cgctagattt
      601 gaacccggat ttacgcgttc ttgaaaaagc ttggattgat ttccaattta ttggtatcaa
      661 taaaggtggt ggcgttgtag accgtaccca gaagatcgaa aagatggtga cttactacaa
      721 agcatgtccg taatagataa taaaaaagcg accccacttg gggtcgcttt tttattagtg
      781 gtgattggct ccatcatttg attcatgatt tggtccatcg tcgtttttct tagttttgcc
      841 tgctttttgg gctttctcac acgccttttt ctgtttcttg tcttttatct tttcacattt
      901 accgctgtat tgagcagatt gaatatgttg tgtgccagat tgtgcataag cgttcattgg
      961 catgcttaga cccaaagctc caagcatgag aaggatggat gcaacggtct taaaaacgat
     1021 agttctgatc atggtactct cctatttact ggtgatttaa taggatagtc ggtagtattt
     1081 gataaaagtt atgggtgtga attaaagcac aagcggcgag gaacttcctc gccgcttgta
     1141 tgtgcgattt atttgtcaag caaccgcttc aggctttttc attcgctcac gtccccagag
     1201 ccagagtgcg cccaaaccga agaacatgat taagaatccg atcaaccaac cgaccaaagg
     1261 taatttgacc aataagccaa caatgaccac gccgagcaaa agaggccaga ccttgtgttc
     1321 agccaatgcg gggttgaagc gaccaagaat ccatttgcca cctaaccatg caacaaggat
     1381 ctgagcaccg aaaccggtga acaaagcgaa cccaacaatc attgcgaaaa tgctcagcaa
     1441 accaacccag atgatggtac cggttacacc accaagagag attacaccga agatgatgcc
     1501 gcctacaatc attacgatta cgatcaatag gattgcgaag aagaaagcag cataggcaac
     1561 gatgccccag ccaaagctcg gaataggctg actctgaagc ttatctccaa gacctttcaa
     1621 gaagcctggc acaagccaga ccaacaaaag cccaaagatg gcaagcgttg caatcttgcg
     1681 cagcaagtca aatacccatg tagctgcaac ttcacctgct gttggcggag tgggaacact
     1741 ttgcgtatca acagtcggtt ctttgcgagt gacttttcct tcaatagcat cagaagggaa
     1801 cttaatgtcc ttcgattggg tgtatgtaaa atcaccacca atttttgcac tttcagtaac
     1861 ggtcaaaccg ggctgaacgc taggcgtaga aacctttaca tccccaatat acatggaagg
     1921 agacgggctg ccttcctgca tttccccaac ttcagcttgc acatccccgc cgatctcacc
     1981 actcaactcc aaggcgccag tacccaccca gagattacgt ccgatcgaac cagcgagcaa
     2041 actttgtccg ccagcaaaaa caacatcctg ccccacagca ctgccatttt gggcttcaag
     2101 gcttgcacca gctgaaatta aatctccgcc gatagacgcc ttctcaccca gctgtaaagc
     2161 cgcaccggca atacgggcat catcttttac agtgccatcg atcacaatcg tatttcctgc
     2221 ggcgatcaaa tcaccttcca cggtaccatt gatgaagata taagcaccaa acaccaccaa
     2281 atcccctttg acggttccgt ctaaagtgaa ctcttctgcg ctaacatata agtcatcatt
     2341 aatgacttca tctgcggcaa taacgatttt atcgcccgaa cgcccatcaa atgccaaggc
     2401 aggcgaggca aatgttagag agaacaaggc aactaggaca aacattgcca aaaatctttg
     2461 aattgttttc ataaatactc ctttgataga aacttcctga ggaggatata cgcccgaata
     2521 tcccaaaggt tgcagagatg cgccctcgcc tctttctcag ctcgtagttg acataaccta
     2581 ttgaccttta atgactttga agcctatagt tccttcaatt ttgcgggcag accaaaatac
     2641 caattcatta attggataaa ccaataacaa taaagctcag gagattggga aagcccagcc
     2701 ttgaccacat ccacactgac gaatcgaact tcactaatct ctaccgggtc aaactgcacg
     2761 gattttccat tgagcctgcc ctcgtaaatc tcactgatct c
//
LOCUS       DAOREF010000379         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24812_length_2800_cov_3.134791, whole genome shotgun sequence.
ACCESSION   DAOREF010000379 DAOREF010000000
VERSION     DAOREF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24812_length_2800_cov_3.134791"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1448)
                     /locus_tag="PKI33_08690"
     CDS             complement(81..1448)
                     /locus_tag="PKI33_08690"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37127.1"
                     /translation="MATEAIVNDRKEIFGWAMYDWANSAFSTTVGTVFLGPYVASLAR
                     AAAEAAGTSTVSFLGIPVAPDSFLPYCISFSVGMQVLFLPILGAIADYSHLRKTMMRI
                     FATIGAIATILMFMLTGGLWWLGGVLFILANLTFGAAIVFYNAYLPDIASEEERDRVS
                     SYGWAMGYLGGGILLALNLAFYIFSDKIGVPGDLAVRINLASAGIWWLGFSFFTWNRL
                     RSRHASNSLPAGETYASIGFKQLRKTFSEVRHFPETMKFLLAYFLYNDGIQTVIAVSA
                     TFAAAPLIQGGLEQDQTTLTAVILMIQFMAFFGALFWGKLAGWIGAKQSVIVSLVIWA
                     GVVIYAYGGLKGDSVTAQFFILGVFIALVMGGSQAISRSLFAQMIPNGKEAEFFAFYE
                     ISERGTSWIGPLVFGLANQMFNNLRVGILSLIFFFIAGLILLPFVNVSKAIADAKKYD
                     KNAAI"
     gene            1699..>2800
                     /locus_tag="PKI33_08695"
     CDS             1699..>2800
                     /locus_tag="PKI33_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNM37128.1"
                     /translation="MFETLTGRLNQIFDNLRKRGKLSEADVDAAMKEVRLALLEADVH
                     FSVVKTFIAKVRERAVGAEVSKALNPGQQVIKIVNEELVATLGEPAPLNLTGPKPRVV
                     MMVGLQGAGKTTVTGKLARLLKSKGERILMVAGDPYRPAAVKQLQQLGERVGVDVEAD
                     PSIKPQELAKRALDKAEKGGYSLMLVDTAGRSQMDAQLMDELKAIHANVKPVDTLLVI
                     DSMIGQEALNIAQGFKETIDLTGLILTKMDGDSRGGAAISIRSVTGVPIKFIGTGEKL
                     DALETYDPSRLSSRILGMGDLIGLIEKAEAAYDAETAEKQAKKLMKGDFSLEDWLEQM
                     NQMKKMGPLSQIMEMLPGQMGQAARGIDPSLVE"
BASE COUNT          792 a          712 c          671 g          625 t
ORIGIN      
        1 tccattgttt ataaagaaaa caaagcatca acattaaata aaaagagtgc cgagaaattt
       61 ctcggcactc tttttattat ttagatagcg gcattcttgt cgtacttttt tgcatccgca
      121 atggctttgc tcacattcac aaaggggagc aagatcaagc ctgcaatgaa gaagaaaatc
      181 aaagacaaga tgcccacgcg taggttatta aacatctgat tggcaagacc gaaaaccaac
      241 ggtccgatcc atgaagttcc gcgttcactg atctcataga aggcgaagaa ttctgcttct
      301 ttgccgttcg ggatcatttg agcgaacaaa ctgcgactga tggcttgtga accacccatg
      361 accaaggcaa tgaacacgcc caaaataaag aactgagccg taacgctatc gcccttcaac
      421 ccgccgtagg cgtaaatcac tacacctgcc caaatgacca agctcacaat caccgattgc
      481 tttgcaccga tccagccagc caacttgccc cagaacaatg cgccaaagaa tgccatgaat
      541 tggatcatca agatcacagc ggtgagagtc gtctggtctt gttccagccc gccctgaatc
      601 agaggcgcgg ctgcgaaggt tgcagaaacg gcgatcacgg tctgaatgcc gtcgttgtat
      661 aagaagtatg ccaaaagaaa tttcatcgtt tcagggaagt gacgtacttc gctgaaggtt
      721 tttctcaact gtttgaaacc aatgctggca taggtctcac cggcaggtaa ggagttcgac
      781 gcatggcgtg agcgcaaacg attccaagta aagaacgaga agccaagcca ccaaataccc
      841 gccgatgcaa ggttgatgcg cactgccaaa tcgccaggca caccgatctt gtcactgaaa
      901 atataaaaag ccaggttaag cgccagcaaa atgccgccac ccaaatagcc catcgcccaa
      961 ccatacgaag agacacggtc tcgttcttct tcgctggcaa tatcaggtaa ataggcgtta
     1021 tagaaaacaa tagcagcacc aaaggtcagg ttggcaagaa taaagagcac accgcctaac
     1081 caccaaagcc cgcctgtgag catgaacatc aaaatagtgg caatcgcgcc gatggtcgca
     1141 aagatgcgca tcatggtctt gcgcaagtgc gagtaatctg caattgcacc aaggattggc
     1201 aggaacaaaa cctgcatgcc tacagaaaat gaaatacagt acggtagaaa agaatctggt
     1261 gcaacaggaa ttcctaagaa cgaaacggtg ctggttccag ccgcttctgc tgcagcgcgc
     1321 gccaaacttg ccacatacgg tcctaaaaat acggttccaa cagtcgtgct aaaagccgag
     1381 tttgcccagt cgtacattgc ccacccaaaa atctctttcc ggtcattgac gatcgcttcc
     1441 gttgccatgc ctcttctcct cgaactggat tgatgttatg gattcacgtc tgcccgtatt
     1501 atatacggac aacccacaga tgttcggtta acattgtgag aataaaaacc ctcgtctctg
     1561 tgaatggttt tgccaatttt tatggattaa gaaccgcgcc tcctgcttga ccctcaacct
     1621 gctccgcgct aaaatgcctc ttcttacact gaaaaggaat cttgccattt tgtcttaatg
     1681 gcagtcattt atacccctat gtttgaaact cttaccggac gtctcaacca aatcttcgac
     1741 aatttgcgca aacgcggaaa gctctccgaa gccgatgtcg atgccgccat gaaagaagtg
     1801 cgcctcgcct tgctcgaagc ggatgtgcat tttagcgtgg tcaaaacttt cattgccaaa
     1861 gtccgcgaac gtgcggtggg cgcagaagtt tcgaaggcgt tgaacccggg tcagcaagtc
     1921 atcaagatcg tcaacgagga acttgtcgcc accctgggtg aacccgctcc gctaaacctc
     1981 acgggaccca aaccgcgtgt ggtcatgatg gtcggtttgc aaggtgctgg taaaacgaca
     2041 gtcacaggca agctggcacg tttgctcaaa tctaaaggtg aacgcatttt gatggtggct
     2101 ggcgatcctt atcgcccggc ggcggtcaag cagctacagc aactcggtga acgagttggc
     2161 gtggatgtag aagcggatcc ttctatcaag ccgcaggaac tcgcgaagcg cgcgttagat
     2221 aaagccgaaa agggcggcta tagcctgatg ttggtcgata cagccggtcg gtcccagatg
     2281 gatgcgcagt tgatggacga attgaaagcc atccatgcca atgtcaagcc ggtggatact
     2341 ctgctcgtca tcgattccat gatcggtcag gaagctttga acattgctca aggtttcaaa
     2401 gaaaccatcg acctgacggg acttatcctc accaaaatgg acggcgactc acgtggtggt
     2461 gctgccatct ctattcgttc ggtgaccggc gtgccgatca aatttattgg tacaggtgaa
     2521 aagctcgatg cattggaaac ctatgaccca tcacgtttgt cttcacgtat tttgggcatg
     2581 ggcgacctca tcggcttgat cgaaaaagcc gaagccgcct acgatgccga aaccgcagaa
     2641 aagcaagcca agaaattgat gaaaggcgat ttctcgcttg aagattggct tgaacaaatg
     2701 aaccagatga aaaagatggg tccgctttcg cagattatgg aaatgctacc cgggcaaatg
     2761 ggacaagctg cccgtggcat tgacccttca ctggtagaaa
//
LOCUS       DAOREF010000380         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24844_length_2798_cov_4.601531, whole genome shotgun sequence.
ACCESSION   DAOREF010000380 DAOREF010000000
VERSION     DAOREF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24844_length_2798_cov_4.601531"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKI33_08700"
     CDS             complement(<1..309)
                     /locus_tag="PKI33_08700"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37129.1"
                     /translation="MNYQLGLIGYPLGHSLSPKIHTAALVACGLQGTYSLFPIHPDNK
                     QGLHNLLAQVRAGEIRGLNVTIPHKQNVIEFMDELTPTAKAIGAVNTIYMRADKLIGD
                     N"
     gene            complement(320..1621)
                     /gene="aroA"
                     /locus_tag="PKI33_08705"
     CDS             complement(320..1621)
                     /gene="aroA"
                     /locus_tag="PKI33_08705"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNM37130.1"
                     /translation="MNIHHSSFRIHPLSHPLSATVRVPGSKSLTNRALLISSLANGTT
                     HLTNTLFSDDSHYFAKALQTLGFNVQLDEENFSMTVTGMGGKIPARKAELFIGNAGTA
                     ARFLSAFLTLGHGEYILDGEPRMRERPIRDLIDSLSQLGAKLEPTNDCPPVKISANGL
                     PGGKTTIAGDISSQFLSALLMVAPYAKSPIEVTLSTDLNSKPYVDMTISIMKDFGVEV
                     ERDGYEYFKVPVTSYQLPVTSYPIESDASAASYFFAAPAICGGTVTVENISHKSVQGD
                     VAFVDVLKQMGCAVEEHDNSILVTRASSLVGIDVDMRDIPDTAQTLAAIAPFASSPTR
                     IRGIASARVKETDRVHATCVGLDGLGVRVEEHEDGMTIYPVEKMRSASIQTYNDHRMA
                     MAFSLIGLRFDGVTIENPSCVSKTFPNYFEVLEELRTQGNK"
     gene            complement(1618..2484)
                     /locus_tag="PKI33_08710"
     CDS             complement(1618..2484)
                     /locus_tag="PKI33_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNM37131.1"
                     /translation="MSTPMSQPDFNLAESKIAIIGLGLMGGSLALGLRGKCAALYGVD
                     PHLPTLELALSQHIVDYTDSNPAKLLPEVDLVILSAPVPAILTLLEKLPSFTSNPCIV
                     MDLGSTKRMVVEGMANLPERFDPIGGHPICGKEKLSLANAERTLYYAAPFLLTPLERT
                     SQRAISAANQIIEALGAKGKILDASEHDRILAATSHLPFLISSALTLATPEEVAPFVG
                     PGFKSTSRLAGTSSSMMLGVLQSNRENVLEALHRMQNEFAQLEAALTAGDFVKVESLL
                     NKAQAKHQSFTQ"
     gene            complement(2459..>2798)
                     /locus_tag="PKI33_08715"
     CDS             complement(2459..>2798)
                     /locus_tag="PKI33_08715"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNM37132.1"
                     /translation="ILCERGIRTFETATRNTTDINAVPVLKKLTHLPIVMDPSHSTGD
                     SAYVQSIARASVAAGADGLIVEVHQDPPHAISDGKQSLTPEAFAKMVKQVKAVAEAVD
                     RKLVNSHVAA"
BASE COUNT          682 a          617 c          810 g          689 t
ORIGIN      
        1 attatcgcca atgagtttat ctgcacgcat ataaatcgta ttgaccgcgc cgatggcttt
       61 ggcggtgggc gtgagttcgt ccatgaattc gatgacgttc tgtttgtgcg gaatggtgac
      121 gttgagtccg cgaatttcgc cagcacgaac ttgggcgagc aggttgtgca agccttgttt
      181 gttgtcaggg tgaataggga atagggaata ggttccttgt aagccgcaag cgactaaagc
      241 cgctgtatga atctttggcg atagtgagtg tcccaaggga taaccgatta aaccaagttg
      301 ataattcatc atcacctgtt tacttgtttc cttgggtacg caattcttca agcacttcaa
      361 agtaattagg aaatgtcttt gaaacacaag atggattttc gatggtgaca ccatcaaagc
      421 gtaagccgat gagagagaat gccattgcca tgcgatgatc gttataggtt tggatggaag
      481 cgctgcgcat cttttctacg ggatagatgg tcattccatc ttcatgctct tcaacgcgga
      541 cgccgagtcc gtccaaccca acacaggtcg cgtggactcg atctgtctct ttgactctgg
      601 cagatgcgat gccacgaatt ctggttggtg atgaagcaaa tggggcgatc gcagcaagag
      661 tttgggcggt gtcgggaata tcgcgcatat ctacgtcaat gccaacaagc gacgaggcac
      721 gagtaacgag tatcgaatta tcgtgctctt caacagcaca acccatttgt tttagcacat
      781 ctacaaaagc aacatcgcct tgcacagact tgtgcgagat gttttctact gtgactgtcc
      841 cgccgcagat ggcaggggcg gcgaagaaat aggacgcagc ggaggcgtca ctttcaattg
      901 gataactggt aactggtaac tgataactgg tcactggcac tttgaaatat tcatatccat
      961 cccgttcaac ttccacaccg aagtctttca tgatggaaat tgtcatgtct acgtagggct
     1021 tagagttgag atcagtggag agagtgactt caatagggct tttagcataa ggagcgacca
     1081 tcagcaatgc ggataagaat tgagaagaaa tatcaccagc aatggttgtc tttccaccag
     1141 gcaagccatt tgctgaaatc tttacgggtg gacagtcatt tgtcggttcg agttttgcgc
     1201 caagttgtga caacgaatca atcaaatccc ttattgggcg ttcgcgcatg cgcggttcgc
     1261 catccaaaat gtattcgcca tgcccaagtg tgaggaacgc ggagagaaat cttgccgcag
     1321 tgcccgcatt gccgataaag agttcggctt tcctggcagg gattttccct cccatgcccg
     1381 tgaccgtcat ggaaaaattt tcttcatcca attgaacatt aaagccgagg gtttgcaggg
     1441 ctttagcgaa gtaatgcgag tcgtcggaga agagtgtatt ggtgaggtgg gtcgtgccgt
     1501 ttgcaaggga cgaaatcagc agggcgcggt tggtcaatga cttggagcca ggtacgcgga
     1561 cggtggcgga gagaggatga gacaaaggat gaatgcggaa tgatgaatga tgaatattca
     1621 ttgagtgaaa gattggtgtt tggcttgcgc tttgtttaga agggattcga cttttacaaa
     1681 atcgccagcg gttagtgcgg cttcgagttg agcaaattca ttttgcattc gatgcaacgc
     1741 ctccaacaca ttctcgcggt tggactgtaa aacgcccaac atcatggagg aagatgtgcc
     1801 agcaagtctg ctcgtggact tgaagccagg cccgacaaag ggagcgactt cttcaggggt
     1861 tgccaatgtc agcgcagatg agatcaagaa aggcagatga ctcgtcgcgg ctaggatgcg
     1921 gtcatgttct gaagcatcaa ggattttacc tttggctcct agcgcttcga tgatttgatt
     1981 ggcagcagag atggcacgtt gtgaagtacg ttccagcggg gtgagcagaa atggcgcggc
     2041 gtagtagagg gtccgttcgg cgtttgcgag cgagagtttt tctttgccgc aaatgggatg
     2101 cccgccgatg gggtcgaagc gttcgggcaa gtttgccatc ccttcaacca ccatccgttt
     2161 tgtggagccc aagtccatca caatgcaggg gttggatgtg aacgagggta acttctccag
     2221 cagagtcaaa atcgcaggga cgggtgcaga caggattacc aaatccactt cggggagcag
     2281 tttggcaggg ttgctgtcag tgtagtccac gatgtgttga gacagagcca actcaagcgt
     2341 ggggagatgc gggtcgactc catagagcgc ggcacatttt ccacgcaatc ccaacgccag
     2401 tgaaccgccc atcaagccca atccgatgat ggcgatcttt gattctgcga gattaaagtc
     2461 aggctgcgac atgggagttg acaagtttgc ggtccaccgc ttcagccacg gctttgacct
     2521 gcttcaccat tttggcaaag gcctcagggg tgagcgattg cttgccatcg gaaatggcgt
     2581 gcggcgggtc ttggtggact tcgacaataa gaccgtcggc acctgcggca acgcttgcgc
     2641 gggcaatgga ttggacatac gccgagtcgc cggtggagtg agatggatcc ataacgatgg
     2701 gcagatgtgt gagcttcttg aggacaggta cagcattgat gtcggtcgtg ttgcgggtgg
     2761 cggtttcaaa ggtgcgaatg ccgcgctcgc acaagatg
//
LOCUS       DAOREF010000381         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24863_length_2797_cov_3.611962, whole genome shotgun sequence.
ACCESSION   DAOREF010000381 DAOREF010000000
VERSION     DAOREF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24863_length_2797_cov_3.611962"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKI33_08720"
     CDS             complement(<1..542)
                     /locus_tag="PKI33_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNM37133.1"
                     /translation="MATPNNLVYERPEAFTEMPTHYCPGCTHGVAHRLVAEVLDEMNL
                     VEKTIGVAPVGCSVFAYNYFDTDFVEAPHGRAPAMATGVKRVLPDRVVFTYQGDGDLA
                     SIGMGEIVHAAARGENITVIFINNANYGMTGGQMAPTTLPGMKTSSSQSGRDVETQGY
                     PIKVSEMISTLDGVGYCVRRS"
     gene            complement(542..1648)
                     /gene="vorB"
                     /locus_tag="PKI33_08725"
     CDS             complement(542..1648)
                     /gene="vorB"
                     /locus_tag="PKI33_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNM37134.1"
                     /translation="MPKELWKGNEAIAESAVRAGLGAYFGYPITPQTEVLEYLSRRMP
                     ELGRAFVQAESELGAINMVYGAACTGVRVMSSSSSPGMSLMMEGLSYIAGTEIPAVLV
                     NVMRGGPGLGNIAPAQSDYNQAVRGGGHGDYPRIVLAPASVQEAIDLTALSFDLAEKY
                     RMITLIILDGTTGQMMEPAEMPAMQEMHRKNFEWATNGAMNRERRVLSSIYLNPLDEE
                     QANLRMLRNWKNVQANEVRYKEYFLDDAKYVVVGFGTAGRVALSAVREARAQGIKVGL
                     LRPITVSPFPYEALEQLIPQAEAFLVAEMNMGQMLDDVLMTVRGRVPVEFYGRPGGVV
                     PFHNEILDEIHRLASGPLAVTTDPRDSWLTRMTA"
     gene            complement(1702..1944)
                     /locus_tag="PKI33_08730"
     CDS             complement(1702..1944)
                     /locus_tag="PKI33_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNM37135.1"
                     /translation="MAAKGWIEVSDTYCKGCELCISACPQNVMELNMEHLTPKGYHPA
                     HTFKDGCTGCAICAVVCPDAAITVYREVTKAAPVAA"
     gene            2166..2414
                     /locus_tag="PKI33_08735"
     CDS             2166..2414
                     /locus_tag="PKI33_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37136.1"
                     /translation="MENLKEWLLGLDTMILVILFLGGFIAWELKSGEVPLRWFGSIRR
                     DARPFFYWIAMLFHLAILGIVVYAYTDGVRMPLAEFFQ"
     gene            complement(2446..>2797)
                     /locus_tag="PKI33_08740"
     CDS             complement(2446..>2797)
                     /locus_tag="PKI33_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37137.1"
                     /translation="YEFAQREKGVTINVCYPGQASTNMTRGVTPDMLPRAMRWMFPIF
                     KLAVREDGGKSAAKAARSSVYLTSSPEVEGVTGKYFDTNCKEAAWSPAVMDEANREML
                     WKIVERLTFATKEH"
BASE COUNT          651 a          724 c          704 g          718 t
ORIGIN      
        1 gagcggcgca cacaatagcc gaccccatcc agtgtggaaa tcatctcaga tactttgatg
       61 ggataacctt gtgtttccac atcacgcccg ctttgagaag aggaggtctt catgccgggt
      121 aaggtcgtcg gtgccatttg cccgccggtc atgccatagt tggcgttgtt aataaagata
      181 acggtgatgt tctctccgcg tgctgccgca tgcacaattt cgcccatgcc gatggatgcc
      241 aagtcaccat cgccttgata agtgaatacc acacggtctg gcagtacgcg cttcacaccg
      301 gttgccatgg cgggcgcacg tccgtgcggg gcttcaacga aatcggtgtc aaagtagttg
      361 tacgcaaaga cggaacagcc taccggtgca acaccgattg tcttctcaac cagattcatt
      421 tcatctaaca cttcggctac cagtctatgt gccacgccgt gcgtacagcc cgggcaatag
      481 tgggtgggca tttcggtgaa agcttcgggt ctttcgtaaa caagattatt tggagtagcc
      541 attatgcagt catccttgtc agccaggaat ctcttgggtc tgtggtaaca gccaaaggtc
      601 cagaagcgag gcgatgaatt tcatccaaga tttcattgtg gaagggaacc acgccgccgg
      661 gtcgcccgta aaactcaacc gggacacgcc cgcgaacggt catcaacaca tcatcgagca
      721 tttgccccat attcatttca gcgacgagga acgcctcagc ctgcgggatg agttgttcca
      781 gcgcttcata cggaaacggg ctgacagtga tcggtcttaa caaaccaacc ttgatgcctt
      841 gtgcgcgcgc ctcgcggacc gccgagagcg caacacgccc cgccgtgcca aagccgacga
      901 caacatattt ggcatcgtcg agaaaatatt ctttgtaacg aacttcattg gcttgcacgt
      961 tcttccagtt tctcaacatg cgcaggttgg cttgttcttc atccaatggg ttgaggtaga
     1021 tcgaagaaag cacacggcgt tcgcggttca tcgcgccgtt ggttgcccac tcaaaattct
     1081 tgcgatgcat ttcttgcatg gcgggcattt cagccggctc catcatctga ccggttgtgc
     1141 cgtcgagaat gatgagcgtg atcatgcgat atttttcagc gaggtcgaag gagagcgcgg
     1201 tcaagtcaat ggcttcttgc acggatgcgg gagccagcac aatgcgtgga taatctccgt
     1261 gcccgccgcc acgcacagct tggttgtaat cactttgagc cggggcaata ttgcccaagc
     1321 cgggtccgcc gcgcatcacg ttgaccaaca ctgccgggat ttctgttcct gcgatataag
     1381 acaacccttc catcatcagg ctcatgcccg gtgaagagga ggaagacatc acgcgcacgc
     1441 ctgtacatgc cgcgccatag accatgttga tcgcgccaag ttcagattcg gcttgaacaa
     1501 aggcacgtcc cagttcgggc atacggcgcg agagatattc gaggacttct gtttgtgggg
     1561 tgattggata cccaaaatat gcccccaacc cagcccgcac agcagattct gcgatggctt
     1621 cattgccttt ccataattct tttggcattc gtttctccat gtaaccggta agggcgactc
     1681 gcgagtcgcc cttacgaata attacgccgc aaccggcgca gccttggtca cttcacgata
     1741 gacggtaatc gccgcatctg ggcagacgac cgcacaaata gcacaccccg tgcaaccgtc
     1801 cttgaacgtg tgcgcggggt ggtatccctt cggagtgagg tgctccatat tgagttccat
     1861 cacattttgt ggacaggcac ttatgcaaag ttcacagcct ttgcaatagg tatcgctgac
     1921 ttcgatccaa ccttttgctg ccatcacagc tccttatatt gagaagttag acactattag
     1981 gcgactttca tcgctaagtc tattatgtcg ttttcggaaa tcgaggagta gtgtcaggcg
     2041 tcatgtacaa gcgagtacaa aggtcattcg tttttcggtc aactacaagg acttaagagc
     2101 gacttgaaca gacaatttca gagctctgag ataggtataa tttcaaaaaa tttaggagtg
     2161 tatctatgga aaacttaaaa gaatggttac tcgggttaga cacgatgatt ttagttattc
     2221 ttttcttggg tggctttatt gcgtgggaat taaaatctgg tgaagtccct ttgcgttggt
     2281 ttggttcaat tcggcgtgat gcccgtccat ttttttattg gatcgccatg ctgtttcatt
     2341 tagcaattct aggcattgtg gtatatgcat atacagatgg tgttcgaatg cctttagcag
     2401 aattttttca ataaaaaaga gccggaattg cggctctttt ttatctcaat gttcttttgt
     2461 tgcgaatgta agccgttcaa caatcttcca tagcatttcg cgattcgctt catccatcac
     2521 tgcaggtgac cacgccgctt ctttacaatt cgtatcgaaa tatttccccg tcactccttc
     2581 gacctcaggc gaagaggtga gatagaccga cgagcgcgcc gccttcgccg cagatttgcc
     2641 gccgtcttca cgaacagcta atttgaaaat ggggaacatc cagcgcatag cacgcggtag
     2701 catatcgggc gtcacaccgc gggtcatatt ggtactggct tgcccagggt agcagacgtt
     2761 gatcgtcacg cctttttcgc gctgggcaaa ctcgtac
//
LOCUS       DAOREF010000382         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24874_length_2796_cov_4.045239, whole genome shotgun sequence.
ACCESSION   DAOREF010000382 DAOREF010000000
VERSION     DAOREF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24874_length_2796_cov_4.045239"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKI33_08745"
     CDS             <1..346
                     /locus_tag="PKI33_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNM37138.1"
                     /translation="LSEIERQIEEIKGRINYLQDRSSFSTITVNIEPKLPDYVPPTPT
                     ATPSPTPTPKDPWKPGETAEKAIGTVTWAYQGIINMLIWFVLVLVPIFAPPILILWLI
                     IKWIKRKPNKPS"
     gene            complement(392..2188)
                     /locus_tag="PKI33_08750"
     CDS             complement(392..2188)
                     /locus_tag="PKI33_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNM37139.1"
                     /translation="MPNEFHYKWEWDLASSPEALWPLASDTNRFNRDTGLPPMQLLGI
                     QNRVKQVKFKVPIVNIVWDEEPFEWTYPYRFGILRRYRTGPLQEMRVDCRLERRDAGT
                     HLIYEVWVKARNVLGDIAIPLGIGTYSAKRFEDAFKLYDRIASTSNTLPGTTTKQNLS
                     SSGHSRYRLLRERLIAQNVDQTTLDRLYEYFQHADDLSIQRMRPYALADGWNLPRRAV
                     LEAFLFATREGILDMYWDLLCPECRGVAEDHKRLGDLHAHSHCNTCQIDFNVNFDQNV
                     EVIFRPNPSIRQVDTSIEFCVGSPQRQPHIVFSLIVPPREQLPFNTMLKAGRYRLTAS
                     AVDGSQILTAASDGPATMDFHADKLGWNSDFLKIGLSPNVRLFNDSDFTQTFQMERVV
                     WSDQAATAADVTTLQVFRDLFSNEVLRVDEEISVGSMTLMFTDLRNSTKMYREIGDAP
                     AFGRVREHFEILEQAVAKEEGAIVKTMGDAVMAAFRKPVSAVRAIWDVQRELTARGIP
                     PLSIKVGLHHGPCIVVNLNERLDYFGSAVNIAARLPHFSAGGELVLSGEIRHDPEVSE
                     FLAKNAAPNTVSRFQSELRGYDTPMEMWRVRI"
     gene            complement(2261..>2796)
                     /locus_tag="PKI33_08755"
     CDS             complement(2261..>2796)
                     /locus_tag="PKI33_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37140.1"
                     /translation="AGIRVVGEATRLSDVNEHETEVAVLASVSSVRSVDQKDFCILFL
                     TDDIESVRPLLSSGMSAWGVLSSEANADELAAGIAAVAEGLWVGAPGLVEGLMRLPKG
                     GEGMGEESLPEPLTAREKEVLQHMAEGLANKQIALALRISEHTVKFHLSALYAKLGAA
                     SRTEAVKRGIELGLISL"
BASE COUNT          760 a          766 c          609 g          661 t
ORIGIN      
        1 actctccgaa atcgaacgtc agatcgaaga gatcaaaggg cgtatcaatt acctgcaaga
       61 ccggtcttct ttctcaacaa tcacagttaa catcgaaccg aaactgccag attatgttcc
      121 gcccacacct acggcaacgc ccagccccac ccccacaccg aaagacccgt ggaagcccgg
      181 cgaaaccgct gaaaaagcca ttggaactgt cacctgggca taccaaggca tcatcaatat
      241 gctgatctgg ttcgtactgg tactggtacc gatctttgca ccgcctatat tgatcctttg
      301 gctcattatc aagtggatca aacgaaaacc aaataagcct tcctaaaaca aaaagcgccg
      361 ggtttgcact cggcgctttc tttataagtc tttatattct cacccgccac atttccatgg
      421 gtgtgtcata accgcgtaat tcagattgaa agcgagagac tgtattcggc gctgcatttt
      481 ttgcaaggaa ctcgctcact tccgggtcgt ggcggatttc tccagagagg accaactccc
      541 ctcctgccga aaagtgtggg agccgcgccg caatattcac agccgaaccg aaataatcca
      601 agcgttcatt taagttgacc acaatacacg gtccatgatg caagcctact ttgatgctca
      661 atggtgggat gccgcgtgcg gttagctcac gttgaacatc ccagatcgca cgcaccgccg
      721 aaaccggctt gcggaacgca gccatgactg catcacccat ggttttgacg atagcacctt
      781 cttcttttgc cacagcttgc tccaagatct caaaatgctc acggactcgc ccgaatgcag
      841 gagcatctcc gatttcacga tacatctttg tagaattgcg caaatcagtg aacatcaaag
      901 tcatcgaacc gactgaaatc tcttcatcca cacgcaagac ttcattagag aataggtcac
      961 ggaatacttg taaggtcgtc acatctgctg cggtggcagc ctggtctgac caaacaactc
     1021 gttccatttg gaaggtttgg gtaaagtctg aatcattgaa taggcgaaca ttcgggctca
     1081 gccctatctt taagaaatca ctgttccaac ctaacttatc ggcatggaaa tccatcgtag
     1141 cgggaccatc actggcagcg gttaatatct gtgatccatc tactgccgaa gcagttaagc
     1201 gataacgacc cgccttcaac atggtattaa aaggcaattg ttcacgcggc gggacgatca
     1261 acgagaaaac aatatgaggt tggcgttgtg ggctgcctac acaaaactca atcgatgtat
     1321 ctacttgccg aatagatggg ttcgggcgga agatcacttc tacattctgg tcaaagttca
     1381 cgttgaaatc gatctgacaa gtattgcaat gcgaatgtgc atgcaaatcg cccaaacgtt
     1441 tatggtcttc tgccacgcct cggcactctg ggcatagcaa atcccaatac atatctaaaa
     1501 tcccttcgcg agtggcaaaa agaaatgcct ctaacacagc acggcgaggc agattccaac
     1561 catctgccaa ggcatagggt ctcatacgct gaatggatag gtcatcagca tgttgaaaat
     1621 attcatacaa acggtcgagg gtggtttgat ctacattttg agcaattagc cgctcgcgca
     1681 atagacgata gcgggaatgt ccgcttgagc tgagattctg tttggtggta gtaccaggca
     1741 gcgtgttaga ggttgaagcg atgcggtcat acaatttgaa tgcatcttca aatcgtttcg
     1801 cactataggt cccgattccc agcggaatag cgatatcccc caagacattg cgggctttca
     1861 cccacacttc gtagatcaaa tgtgtgccag catctcggcg ctccaagcgg caatcgacac
     1921 gcatctcttg cagcggaccg gttcgatagc gacgaagaat tccaaagcgg tacgggtacg
     1981 tccattcgaa aggttcttca tcccatacaa tatttacaat gggcaccttg aacttaacct
     2041 gcttgacccg attttgaatc cccaacaact gcatgggcgg caagccggta tcacgattga
     2101 agcgatttgt atctgacgca agcggccaca atgcttccgg cgaagacgcc aagtcccatt
     2161 cccatttgta gtgaaattcg tttggcatga tatatagacc tataaagtct cattatttta
     2221 cctgccaaac ctgtcaacag cattacagac aaataagaaa ttacaacgaa atcaacccca
     2281 actcaatgcc gcgcttcaca gcttcggtgc ggcttgccgc ccccaatttg gcatacaacg
     2341 ccgaaagatg aaacttaacc gtatgttcgc taattcgcaa cgccagcgca atttgcttgt
     2401 tggcaagtcc ctccgccata tgctggagga cttccttctc gcgtgccgtg agaggctctg
     2461 gtaaagactc ttctcccatg ccttcgccgc cctttggcaa ccgcatcaag ccctcgacca
     2521 agccgggtgc acccacccaa aggccttctg ccactgcagc gattcccgct gctaattcat
     2581 ccgcattggc ttcagaagat aagacccccc acgcactcat ccccgatgat aataaaggcc
     2641 gtacggattc aatatcatct gtcagaaaaa gaatgcaaaa atctttttga tctacactac
     2701 gtacagaaga aaccgaggca agcacagcta cctcggtttc gtgttcattg acatctgaaa
     2761 gacgagtcgc ctcccccacc acacgaatgc ccgctt
//
LOCUS       DAOREF010000383         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_24990_length_2787_cov_5.094070, whole genome shotgun sequence.
ACCESSION   DAOREF010000383 DAOREF010000000
VERSION     DAOREF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24990_length_2787_cov_5.094070"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1166
                     /locus_tag="PKI33_08760"
     CDS             84..1166
                     /locus_tag="PKI33_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37141.1"
                     /translation="MQNRNDFILAGIFLGLGLHGYSPFRMVPIFVVVMFGIYFLHAKT
                     KDERRQAIWWLLIVVVISMFVFLPLLRYWLEYPQNFGYRAFTRLSDTETALPGPAWQI
                     FLSNLYKGLLMFNWDDGEIWVHSVVHRPALDLVSAVFFAVGAIFLIVRYFRAKDWRDL
                     TLLSSIPVLLMPSVLSLAFPGENPSLNRTGGAAIPVILIVAMALDGFLSSFRSEKRRL
                     FIGYGLTFLLLGYSMYLNYDLVFNQFASSYRASSWNTSELGKQLSDFRAKYGQLDTAW
                     IVPFPYWVDTRLPGVWAGVPDRDFAMWQDQLPSSLSYPAPKLFMFWHDDLETEKVLKE
                     LYPNGKLTRYTSAVEGKDFYIFLVEP"
     gene            1194..>2787
                     /locus_tag="PKI33_08765"
     CDS             1194..>2787
                     /locus_tag="PKI33_08765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM37142.1"
                     /translation="MDNKKHFWLYDILFILVFLVAGYLRIVGYDWGEGQHQHPDELFL
                     TGVLDNLRAHTCNDPAIPIDACPEENQRWMTVSEYFDSTTSTLNPYNRGFGFFVYGNL
                     PMTVTRVLMEAIGKDDIGSSKFFARQMSALADLLTIFLLYLIVSRLYGQKIGLLASVF
                     SSLAVMQIQQSHFFTSDLFVNLFMFLALLFAVGLVEWQGRTDDQPEELETVIKVEDST
                     ETAEPVKSSTSTQIQILKHPLFYLTVAFGFALGMAMASKINAAAMALLLPFALAIRWL
                     VYDRKQKLSNEYWMQVAILLMVGGFATLVSFRIFQPYAFDGIGLNAQWVKNISEQRAQ
                     ASGAADLPWNLQWIRRTHLYSFENLTIWGLGLPLGILAWIGFLWMGWRILKGEYKHLL
                     LWGWTAFYFAWQSMQFNPTMRYQLPIYPLLAMMAAWVLFEIAGANAPVVNRSNLRKVS
                     ATVLGLTVLTLTAVWAFAFQSIYLRAEPRIAASRWMFENAPAGINVLIQTKEDTFNQP
                     LPIPSGTLITPENPYSVTFSPQR"
BASE COUNT          644 a          584 c          656 g          903 t
ORIGIN      
        1 taatttcgcg gatagggttg cgcttccctc tatacccttt gtttgttgca ccaacgctat
       61 attatctaat ccgtggcttt agaatgcaga acagaaatga ttttattctt gcaggtatct
      121 ttcttgggct aggcttgcac ggatatagtc ctttccgtat ggttcccatc tttgtggtgg
      181 tcatgtttgg tatttatttt ctacatgcaa agacaaaaga cgaaagacgt caggcgattt
      241 ggtggctatt gattgtggtt gtcatttcaa tgtttgtctt cctgccatta ttgcgttatt
      301 ggcttgaata cccacagaat tttggttacc gagcatttac gcgcttaagc gataccgaaa
      361 cagcattacc cggaccagct tggcagatat tcctttcaaa cctgtataag ggcttattga
      421 tgttcaactg ggatgacggg gaaatatggg ttcactctgt ggtgcaccga ccggcattag
      481 acctggtttc cgcagtgttt ttcgcagtgg gcgcaatctt cttaattgtg cgttacttcc
      541 gcgctaaaga ttggcgtgac ttaaccttac tgagttcaat ccctgtcctg ctcatgccat
      601 ctgtactttc gctggcattc cccggtgaaa acccttcatt gaatcgaacc ggtggtgctg
      661 ctatcccggt gattcttatc gtagcgatgg cgcttgatgg ctttctctca agtttcaggt
      721 ccgaaaagag gcgtttattt ataggatacg ggctgacatt cctcctcttg ggctattcca
      781 tgtatctaaa ttatgatttg gtctttaatc aatttgcgag ttcctatcgc gcttcatcat
      841 ggaatacatc tgaacttggc aaacagttaa gtgactttcg tgctaaatat gggcaattgg
      901 atacagcatg gattgtccca tttccctatt gggtcgatac tcgcttgcca ggagtttggg
      961 caggcgtgcc agaccgtgat tttgccatgt ggcaggacca attgcctagt agcttgagtt
     1021 accctgctcc gaaattgttc atgttctggc atgatgacct tgaaactgag aaggttctta
     1081 aagaactcta tccaaatgga aaattaaccc gctatacttc agcggttgaa ggtaaggatt
     1141 tctatatctt tttggtggaa ccataataat ggttagccct tttacagtga tcaatggata
     1201 ataaaaaaca tttttggttg tatgacattc tgtttattct cgtcttttta gtggcgggat
     1261 atttgcgcat tgttgggtat gactggggag aagggcagca ccaacatcct gatgagcttt
     1321 tcttgacggg tgtgttggat aatctgcgtg cgcacacatg taacgacccg gctattccaa
     1381 tcgatgcttg cccggaagaa aatcaacgct ggatgactgt gagtgaatac ttcgatagca
     1441 ctacgtctac gttgaacccg tataatcgcg ggtttggttt ttttgtttat ggcaatttgc
     1501 caatgacagt gactcgcgtt ttgatggaag caatcgggaa agatgatatt ggttcctcga
     1561 agttctttgc gcggcagatg tctgctctgg cagacctgtt aacgattttc ctgctatatc
     1621 ttattgtgtc gcgtttgtat ggacagaaaa tagggttgct tgcttctgta ttttcttcac
     1681 tagcagtgat gcagatccag caatctcact tttttacttc agatcttttt gtaaacctat
     1741 ttatgttcct tgccctgctc ttcgcagtgg gacttgtcga gtggcaggga cgaacagatg
     1801 atcagccaga agagttagaa acggttatta aggttgaaga cagcactgaa acggctgaac
     1861 ctgttaaaag cagtacttct actcagattc aaattctgaa acatccgctt ttttacttga
     1921 cggttgcgtt tggttttgct ctgggaatgg caatggcgtc caaaatcaat gctgctgcca
     1981 tggcattgct cttacctttt gcgttagcta ttcgctggct tgtttatgac cgaaagcaga
     2041 agcttagcaa tgaatattgg atgcaggtag cgattctttt gatggttggt ggattcgcta
     2101 cccttgtatc ttttcgtatt tttcaaccct atgctttcga cggtataggg ctgaatgcac
     2161 aatgggtgaa gaatatctcc gagcaacgcg ctcaagcatc tggcgcagca gacctgccgt
     2221 ggaatttgca atggattcgc cgtactcatt tgtattcatt cgaaaacctt accatttggg
     2281 ggcttgggct ccctctggga atccttgctt ggataggttt tttatggatg ggctggcgta
     2341 ttctaaaagg cgaatacaaa caccttttgc tatgggggtg gactgctttt tactttgcat
     2401 ggcaatctat gcagttcaac cctactatgc ggtatcaatt gccgatctat ccgttattgg
     2461 caatgatggc agcatgggtt ctttttgaga tcgcaggggc gaacgcacca gtagtaaatc
     2521 gctcaaattt gcgcaaagtt tctgcaaccg ttcttggctt aacagttctt acgcttacgg
     2581 ctgtgtgggc atttgcgttc caaagtattt atttgcgagc tgaaccgcgt atcgcagcat
     2641 ctcgttggat gtttgaaaat gcgcctgctg gtatcaatgt tcttattcaa acgaaagaag
     2701 atacctttaa tcagccttta ccgattccat ctggcacgtt gattacccca gagaatccat
     2761 attcagtgac tttctctccg caaagag
//
LOCUS       DAOREF010000384         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25032_length_2784_cov_5.381092, whole genome shotgun sequence.
ACCESSION   DAOREF010000384 DAOREF010000000
VERSION     DAOREF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25032_length_2784_cov_5.381092"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..257
                     /gene="rrf"
                     /locus_tag="PKI33_08770"
     rRNA            141..257
                     /gene="rrf"
                     /locus_tag="PKI33_08770"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(340..1413)
                     /locus_tag="PKI33_08775"
     CDS             complement(340..1413)
                     /locus_tag="PKI33_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37143.1"
                     /translation="MTLTPSDYREETLKPFFNYIKGGESFFIIGAPSVGKTRLVDFVM
                     GDDSDARRMGIDIDRDRAKKQYLGSAASKVWLVRVDMNRIRLEHSWGFQFFELMLHSL
                     LLACDRNSTISDIEFLKENLAGLDSKVIQSKDDLMAHRLFEMAVNRICQIYNIQICFL
                     FDEFDETYRTMPIETFAQLRAIRDSNKYMLSYILFLRNLPEKLRNPKDNESFYELISR
                     NVLGLGPYAKKDALNIIEQLEKRRDHVLPSEYRDWVYTTSGGHPGLIQALFTLLKENP
                     STPSKIQDPEWFVKQEIIREEFRKIWNGLIEEEQTALQSIAQGDQSLITPSIGKQLIA
                     KGIIKPVGNHIPFFTPLLGYWLK"
     gene            complement(1435..>2784)
                     /locus_tag="PKI33_08780"
     CDS             complement(1435..>2784)
                     /locus_tag="PKI33_08780"
                     /inference="COORDINATES: protein motif:HMM:NF040095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNM37144.1"
                     /translation="LQSLLHPIILTGIEDPTTLRKLLQDYKDITYLGKQDRRDEFRQR
                     LDAEASKVAASKRQLDFVQTELLDEFLKSDLVKSTQEHANQIVDVLARLFPEAKVLRF
                     EKVSGWGETEVSSAVRPNSIVIKVYEDSLEPCLVKLARAKKVDHEVRSYFEYIERKLT
                     GSFNAKLLQKAIVWDIGGAAYTFISGNGTRTFSAYYKDQPIQSIKEVLTSFFRDMWKK
                     YYNQATPEKKSLFNLYLEVWGQDWYEKCANELTSAVLDEELSAISKNMGLPNPIEWLN
                     NKIQKNEMVFDTKIAVTHGDLHGDNLMVDDRKNVWVIDFERCGKGHALQDFIELEADV
                     INRLLGHPVNFPAYLKMCKTVFQTTEINLEEADLVSEDVEIEKALKTISIIRDLATQC
                     TGMNDARQYLYGLLYNMTFRAQLLHKIDKSKSGYPLLLAGLICHRLDHWDQTWSPTE"
BASE COUNT          719 a          557 c          642 g          866 t
ORIGIN      
        1 ggtgtgtaag cacagtaatg tgttcagcta acctgtacta atggtcgagg gcttcatcct
       61 gtgatgcgga gaaattggta cttttccgag aagactacat caaaattgtt attcaggaca
      121 attcatttgt gaatttgtcc cttggtgatt tgagcgatat gggtacaccc ggtcccatcc
      181 cgaacccggc agttaagcat atcagcgctg atggtacttg gagggcagcc tcccgggaga
      241 gtaggtcatt gccaaggggc ttttcttttt ctccgaatag gagaaaaaaa cgccagtcga
      301 aagactggcg ttttagtttt aatctctttt atttattact tatttcaacc aatacccaag
      361 taatggagtg aaaaatggga tgtgattccc aactggtttg atgataccct ttgcaatgag
      421 ctgttttcca atagacgggg tgattagact ttggtcacct tgagcaatac tttgtaaggc
      481 ggtttgttct tcttctatca gcccgttcca aatttttctg aactcttcac gaataatctc
      541 ttgctttaca aaccactcag ggtcttgtat cttggatggg gtggatggat tttctttcaa
      601 aagagtgaaa agagcctgga tcaagccagg gtgacctcca ctagtggtgt aaacccaatc
      661 tcggtattcc gatggaagta catgatcacg gcgtttttcc agctgctcaa taatattaag
      721 tgcatctttt ttggcatacg gacccagtcc aaggacatta cgggagatta gctcataaaa
      781 gctttcatta tctttggggt tgcgaagttt ttctggaagg tttcgaagga aaagaatgta
      841 agaaagcatg tatttatttg agtcccggat tgcccgaagc tgggcaaagg tttcaattgg
      901 catggtgcgg taggtttcgt cgaattcatc gaagagaaag caaatctgaa tattgtagat
      961 ctgacaaata cgattcactg ccatttcaaa tagacgatgt gccattaggt catccttgct
     1021 ttggataact tttgagtcta gccctgcaag gttctctttt agaaactcaa tatctgagat
     1081 ggtactattc cggtcgcatg ccaaaagcag actgtgtagc atcagctcga agaattggaa
     1141 tccccagcta tgctcaagcc tgatgcggtt catatccacg cgcaccagcc agacttttga
     1201 agcggctgat ccaaggtatt gtttcttcgc gcggtcacgg tcaatatcaa tacccatgcg
     1261 gcgcgcatct gagtcgtcgc ccattacgaa atcgactagt cgggtttttc cgacactggg
     1321 agcgccgatt atgaagaaag attcgcctcc ttttatgtaa ttgaaaaagg gttttagtgt
     1381 ttcttcgcga tagtctgaag gggtgagtgt catgatttct ccgtttggtt aagttcattc
     1441 agtgggtgac caggtttggt cccagtggtc gaggcggtga cagattaagc cggcaagcaa
     1501 aagtggatat ccacttttgc ttttgtctat tttatgcaaa agctgagcac ggaaggtcat
     1561 gttatagagc agcccgtata agtattgacg tgcatcattc atgcctgtgc attgggttgc
     1621 tagatcgcga atgatagaga tcgttttgag tgctttttca atttctacat cttccgagac
     1681 taaatctgct tcttctaaat tgatttcagt tgtctggaaa acggtcttac acatttttag
     1741 ataggcgggg aaattgaccg gatgccccaa aagacgattt atcacatcgg cttccagttc
     1801 gatgaaatct tgaagggcgt gccctttgcc gcaacgctca aaatcgatga cccagacatt
     1861 tttgcgatca tctaccataa ggttatcacc atgtaggtcg ccatgggtaa cagctatctt
     1921 ggtgtcgaaa accatttcat ttttttgaat cttattattt aaccattcta ttgggttagg
     1981 aagccccata ttttttgaaa tagcagatag ttcttcatca agaacagcag aagtcaattc
     2041 attggcacat ttttcgtacc aatcttgccc ccatacttcc aaataaaggt tgaacaagga
     2101 ctttttttct ggagttgctt ggttgtaata ttttttccac atatccctga aaaaagaagt
     2161 taaaacttct ttgatgctct gaatgggttg gtctttgtaa taggcagaga aagttcttgt
     2221 gccatttccg ctaataaaag tatatgcggc tcccccaata tcccaaacga tggctttctg
     2281 caggagtttt gcattgaaac tacctgtgag ctttctttca atatactcaa aatagcttct
     2341 tacttcatgg tcaacttttt tcgctcgtgc caatttgaca aggcatggtt caagtgagtc
     2401 ttcatagact ttgattacga tagagtttgg acggacggct gaagagactt cggtttctcc
     2461 ccaaccacta actttttcga agcggagtac ttttgcttcg ggaaagaggc gggcaaggac
     2521 atctacaatt tgattcgcat gttcttgtgt actcttgacg aggtcagatt tgaggaattc
     2581 atccaataac tcggtttgga caaaatcaag ttggcgttta gaggcggcaa cttttgaggc
     2641 ttctgcatcc agccgttgac ggaattcgtc gcgccggtct tgtttgccaa gataggtaat
     2701 atctttatag tcttgtaaaa gtttgcgcaa ggttgtgggg tcttcaatgc ctgtgaggat
     2761 aattgggtga agcaaagatt gtaa
//
LOCUS       DAOREF010000385         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25075_length_2782_cov_4.079575, whole genome shotgun sequence.
ACCESSION   DAOREF010000385 DAOREF010000000
VERSION     DAOREF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25075_length_2782_cov_4.079575"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKI33_08785"
     CDS             complement(<1..331)
                     /locus_tag="PKI33_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37145.1"
                     /translation="MTKRIFISADHGMAIIYFLQSDVVSTLLESGVEVVVLTDDDTKD
                     KISQRFAKPGLFFEGLRLKQANDYAKKVSPRLQSLLIYLRRVGGSRRINTEAMDSHVW
                     EVWGENGW"
     gene            complement(366..1310)
                     /locus_tag="PKI33_08790"
     CDS             complement(366..1310)
                     /locus_tag="PKI33_08790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNM37146.1"
                     /translation="MRKGQNPAKFVKDVARPERVTVALLNYIPFLSGFYAETLDVLKV
                     SLESMRKDAGLPFDLMVFDNGSCEEVREFLIGEKEAGRIQYLILSEKNMGKGGAWNVM
                     LAGAPGEIIAYTDSDVLFSPNWLSRSVELLETFPNVGMVTARPYRTSPELYSSTLEWA
                     KKNATLDEGQFIPWETFLEFNLSLGQTEEENVKVYAETSDWRIQYKGVTALAGASHWQ
                     FTAYKSTLQQFLPFDMDKPMGQVRQLDKRMNDAGLLRLMVSDPLAMNMSNTLGYLRGE
                     LKEERRKKVGVGKRVLEFPPIKKMLLAVYNKIFSWYYS"
     gene            complement(1367..2272)
                     /locus_tag="PKI33_08795"
     CDS             complement(1367..2272)
                     /locus_tag="PKI33_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37147.1"
                     /translation="MSLITLFSAPKPFTDPHIAMIQRSAIKSWTLLPDVDIILLGEET
                     GLAEATKELGVKYIPHVERSPNGVPLISSMFKLARENSNSELFCIINADMVLMPDFVE
                     NAKQAAQLKDKFVLLSQRWDYDITLPIDFADEWQSRLREDVRKQNQIHRPAGSDFFLF
                     PKSCYADIPNFIIGRAGWDNWMIYKARKENWAVIDCTPSVMIVHQNHDYAHLPGGKPH
                     YEHPETNENIRLAGGQANVRYTILDATHQLVDGTLARPKMSSLRFTRKFELFLRTLFF
                     FLPENMVENVARPKRWKKRLKKIIR"
     gene            complement(2323..>2782)
                     /locus_tag="PKI33_08800"
     CDS             complement(2323..>2782)
                     /locus_tag="PKI33_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37148.1"
                     /translation="RLPPPWTRTYPIGLDVEACTFKVLKKAWKEAKEPQHREHAMPYF
                     YEGVQFELKVKGRKSDLGRSTFDLEQGVSSRGFRIALLHHTTDFGDYRWTVDTPEDLE
                     FMREVYSRFDGRDEFTWKEVLDLVHDHPELAKINAGVQHKTLKDIDKRAL"
BASE COUNT          664 a          722 c          679 g          717 t
ORIGIN      
        1 tccagccgtt ctcgccccac acttcccaga cgtggctgtc catggcttcg gtgttaatgc
       61 ggcgcgagcc acccacgcgg cgcaaataga tgagcagaga ttgcaagcgc gggctgacct
      121 ttttggcata gtcgttggct tgcttcaagc gcaaaccttc gaaaaataaa ccgggcttcg
      181 caaaacgctg tgagattttg tctttggtgt cgtcatcggt gaggacgacg acttcaaccc
      241 ccgactcaag cagcgtcgaa acgacgtcgc tttgcaggaa gtagatgatt gccatgccgt
      301 gatcggcgga gataaagata cgtttggtca tatcgtttat tggttaagcc attattggca
      361 attgcctacg aataatacca actgaaaatc ttgttataca ccgccagcaa catcttctta
      421 atcggcggga attccaagac ccttttccct accccgacct tctttcttct ttcttctttt
      481 agttcgccgc gcaaatatcc cagcgtattg gacatattca tcgccagggg atcgctgacc
      541 atcaggcgca gtaaccccgc atcgttcatg cgcttatcga gttggcggac ttgtcccatc
      601 ggcttgtcca tgtcaaaggg caggaattgc tgcagggtgg atttatacgc cgtgaactgc
      661 cagtgacttg cgcctgccag cgctgtcacg cctttgtatt gaatgcgcca gtcactcgtt
      721 tcagcataga ctttcacatt ctcttcttcg gtctgcccaa gcgagagatt gaattccaaa
      781 aaggtttccc acggaatgaa ttgaccttca tcgagagtcg cattcttctt cgcccattcc
      841 aacgtggacg agtacaactc cggcgaggtg cggtacggtc gcgctgtcac catacccaca
      901 ttcgggaagg tttcgagcaa ctccaccgag cgcgagagcc agttgggtga gaacaaaacg
      961 tcgctatcgg tgtaagcaat gatctcacct ggcgcgcccg ctaacatcac attccacgcg
     1021 ccacccttgc ccatgttctt ttcagagagg atcaaatatt gaatccgccc tgcttctttt
     1081 tcgccaatca aaaactcgcg cacttcttcg cacgagccat tatcgaagac catcaggtcg
     1141 aacggcagcc ccgcgtcttt gcgcatggac tcaagcgaga ccttcagcac atcgagagtc
     1201 tcggcataga atccgctcag gaaggggatg taatttaaca acgcaacggt cactcgctca
     1261 gggcgggcaa catctttgac gaacttggca gggttttgac cttttcgcat tttcaatact
     1321 cgttattcgg gattggatat tcgatactcg attgtcgaag atcgaattat cgaattatct
     1381 ttttcaatcg tttcttccat cgcttcggac gggcaacgtt ctcgaccata ttttcaggga
     1441 ggaaaaagaa cagggttcgc aggaacaact caaatttacg tgtgaagcgc agcgacgaca
     1501 tcttcgggcg ggcaagtgta ccatccacta gttgatgggt tgcgtccaaa atggtgtagc
     1561 gtacgttcgc ctgcccgcct gcaaggcgga tattctcgtt cgtttcggga tgttcgtaat
     1621 gaggcttgcc gcccggcagg tgggcgtaat catggttttg gtgaacgatc atcaccgaag
     1681 gcgtgcaatc gatcaccgcc caattttctt tgcgggcttt gtagatcatc cagttatccc
     1741 aaccggcacg accgatgatg aagttcggga tgtcggcata gcaggatttt ggaaagagga
     1801 aaaaatcgct tccggcggga cggtgaatct gattttgttt tctgacatct tctctcagcc
     1861 gagactgcca ctcatcggca aagtcgatcg gcaatgtgat gtcataatcc caacgctggc
     1921 tcaacagcac aaatttatct ttcaactgtg cagcctgttt tgcattctcc acaaaatcag
     1981 gcattaacac catatctgca ttgatgatgc aaaacaattc gctgttcgag ttctcgcgcg
     2041 ccaacttgaa catggacgag atcaacggca caccattagg gctgcgttcc acatgaggaa
     2101 tgtatttcac gccaagttcc ttggtggctt cagcaagccc ggtctcttcg cccagcaaaa
     2161 taatatcaac atccggcaac agcgtccacg acttgatggc gctgcgctgg atcatcgcaa
     2221 tatgcggatc ggtgaatggt ttaggtgcag aaaagagagt gatgagggac ataattacca
     2281 attaccaatt actactgata aatagtaatt ggtcattaga gatcatagtg cgcgcttatc
     2341 gatatctttc aaagttttat gctgtacacc cgcgttaatc ttcgccagtt cagggtggtc
     2401 atgaaccaga tctagaactt ctttccaggt gaattcatcg cgcccatcaa aacgggaata
     2461 aacttctcgc atgaactcaa ggtcttcggg cgtatccacc gtccagcggt agtcgccgaa
     2521 atcggtggtg tggtgcagca gggcgatccg gaatccgcgt gaagaaacac cttgctcaag
     2581 gtcaaaggtc gaacgtccaa ggtctgactt tcgacctttg acctttaact cgaattgtac
     2641 tccttcgtag aaataaggca tggcatgttc acggtgctgg ggttctttgg cttctttcca
     2701 tgctttcttc aagaccttga aagtacaggc ttccacatcc aggccgatgg gataggttct
     2761 cgtccacggc ggtgggaggc gg
//
LOCUS       DAOREF010000386         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25132_length_2779_cov_4.041850, whole genome shotgun sequence.
ACCESSION   DAOREF010000386 DAOREF010000000
VERSION     DAOREF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25132_length_2779_cov_4.041850"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PKI33_08805"
     CDS             complement(<1..1265)
                     /locus_tag="PKI33_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNM37149.1"
                     /translation="MNKKIFMLFSLFVILSMLLAACGGGKQETSQVDTLRVWIQWGDN
                     PQQIQALFDKYTAETGIKVEVTAPLETDKILPALTGSEPPDVLVLSGGDLVKSYYKEG
                     LVDDLNGAITTGGIDLNDFFDAPLNQCRSGDKILCLPWGTDMYALFWNKDLFEAAGLD
                     PETPPATMEELAEFADKLTKVAPDGSLEQIGFLPDMGWDHSDLYINMYGGFWYNEDGT
                     QMTVNSQPVLDALTWQQQFYTKYGVDNVMAFSSGWADTYMSADYPFYTGKLAMYVDGE
                     WMTGPNFINAFKPEASYGVAPFPPPADHPDRANTAVVQGTVAVIPSGAANKEASAKLL
                     AWMMSPEVVAEEFCFNANLPTSKKAAEDPCFTSNPKFKVFTDLISSPNAKIIVTTPIS
                     LEFNDAWGLVIEQVLHAGMDPKPILDDLQA"
     gene            complement(1333..2352)
                     /locus_tag="PKI33_08810"
     CDS             complement(1333..2352)
                     /locus_tag="PKI33_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNM37150.1"
                     /translation="MKNNLSSITIKDVAKHAGVSIATVSRVLNGSVPVQADKAERVRT
                     AMDDLRFVPRTAARTLASRKTNTIGLILSEIGGAFFPLLLKGIESETRAMGYELLIHS
                     TQGAHASQRKTLGEHNTDGLLVFTSSLAAEELERLHKIKFPLVLMHQTPPDGLDMPVV
                     TIENKDGAEMLVSHLIEKHGRRRIVYMRGPEGHEDSLWRERGYREALEKHNLPFAPEL
                     VVMGGFEEEIAFNNIQKMIQEGIEFDAVFAGDDDTAIGVYRALKASKRLIPDDVAVVG
                     FDDVQFSRYISPALTTVRAPIEEVGREAVRQLKSLMDDGQAESLVLMRTELVIRESCG
                     CKNHP"
BASE COUNT          763 a          702 c          592 g          722 t
ORIGIN      
        1 gcttgcaagt catccaaaat cggtttggga tccatacctg cgtgcaaaac ttgttcgatt
       61 accaatcccc aagcatcgtt aaattcgagc gaaatggggg tagtgacaat gatcttggca
      121 ttcgggcttg agatcaaatc ggtgaagacc ttgaacttgg ggttgcttgt gaagcatggg
      181 tcttcagctg ccttcttgct agtgggcagg ttggcattga agcagaattc ttctgccaca
      241 acttcaggag acatcatcca tgccaacaat ttagcagagg cttctttatt cgcagcaccg
      301 ctggggatga ctgccacggt tccttgaaca acggcagtgt ttgcgcggtc ggggtgatcg
      361 gctggcggcg ggaagggtgc aacaccatag ctggcttcag gcttgaaagc attgatgaag
      421 ttcggacccg tcatccactc gccgtctaca tacatggcaa gtttgccggt atagaaggga
      481 taatcagcag acatatatgt atctgcccaa ccagaggaga acgccatcac attgtcaaca
      541 ccatatttgg tatagaactg ttgttgccaa gtgagtgcat ccaacactgg ttggctgttg
      601 acagtcatct gtgttccatc ttcgttgtac cagaagccac catacatgtt gatgtacaag
      661 tcgctatgat cccaacccat atcaggtaag aagccaattt gttcaagcga accatctggc
      721 gcaactttcg tcagcttatc tgcgaactcc gccagttctt ccatcgttgc aggcggagtc
      781 tcagggtcaa gaccggcagc ttcgaacaaa tccttattcc agaacaaagc atacatatcc
      841 gtcccccatg gcaaacaaag gatcttatcg ccgctgcggc attgattcaa aggtgcatcg
      901 aagaaatcat tcaaatcaat ccctccggtt gtaatggcgc cgttcagatc atccaccaga
      961 ccttccttgt aatacgattt cacaaggtcg ccgccgctca ataccaacac atcagggggc
     1021 tccgagccgg tgagcgcggg caaaatctta tcggtttcca aaggcgcggt cacttcaacc
     1081 ttaatacccg tttcagcagt gtatttatca aagagggctt gaatttgctg tgggttatca
     1141 ccccattgaa tccacacacg caacgtatct acctgagacg tctcctgctt gcctccgcca
     1201 catgccgcca acagcatgga aaggataacg aacaggctga atagcatgaa aatctttttg
     1261 ttcatgattc tcctccaggg aaaatgattt ggatacgaca acaaccaaat ggatactctt
     1321 cgccgcacct ccttatggat gatttttaca tccgcacgat tcacggataa ccaactctgt
     1381 acgcatcaaa accagacttt ccgcctgccc atcgtccatt aggcttttca attggcggac
     1441 agcttctcta ccaacttctt cgatcggcgc gcgaaccgtc gtcagcgcgg gagaaatgta
     1501 gcgggaaaat tgaacatcgt cgaaaccgac aacagccaca tcgtcaggaa tgaggcgctt
     1561 ggacgctttc aaggcacggt acacaccgat cgccgtatca tcatcgcctg cgaacacagc
     1621 atcaaactca atcccctctt gaatcatttt ttgaatattg ttaaatgcga tctcttcttc
     1681 aaagccaccc atcacgacaa gttctggtgc aaaaggcaag ttatgttttt ctaatgcttc
     1741 gcgataaccg cgctcacgcc aaagagaatc ttcatgaccc tctggtccac gcatataaac
     1801 gattcgacgg cgaccatgct tttcgatcag gtgactgacc agcatttcag cgccgtcttt
     1861 gttttcaatc gtaaccacag gcatatccaa cccatcaggc ggagtttgat gcatgagcac
     1921 taaaggaaat ttgatcttat gcaaccgttc aagttcttca gctgcaaggc tagaggtaaa
     1981 gaccaacaac ccatcggtat tatgttcccc caatgttttt cgttgacttg catgtgcgcc
     2041 ctgtgtggaa tgaatcaaca attcatatcc catcgcgcgc gtttcagatt caatgccctt
     2101 caacagcaaa ggaaaaaatg caccaccaat ttcagaaaga atcaaaccaa tcgtattggt
     2161 cttgcggctg gcaagagtcc gcgccgccgt gcgtgggaca aaacgcaaat catccattgc
     2221 cgtgcgcacc cgctcggctt tatctgcctg aacaggcacc gaaccattca acacacggct
     2281 aaccgtggca atggaaacgc ccgcatgctt ggcaacatcc ttgatagtaa tagaagacag
     2341 attattcttc atactgttga aagcgttttc tgaaaacgtt ttcaacagta taggcttttg
     2401 aggtcaaaga gtcaagggca actcacccac cagttacaat caggcatgat tactgagatt
     2461 gttactttta ctcaaaacat aaaagtcatg cttgtaaaaa catatcttca tacatacaat
     2521 caatttatat taattgttga agaagtttat cttcgtttga agataatttt tcttcacaca
     2581 aaagggtaat ttgtacatcg atttccatgc ggaaaagggt acccgtatgg atcataaatt
     2641 ttgcctcttg acttagccct tcaattttgt tattatgtta tcgataacat gttatcgata
     2701 acatatatca gtatgtcata aggttattac ccttgaccct acccgatcag aagaggcgtg
     2761 tcacaataaa ggatgtagc
//
LOCUS       DAOREF010000387         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25212_length_2774_cov_3.835601, whole genome shotgun sequence.
ACCESSION   DAOREF010000387 DAOREF010000000
VERSION     DAOREF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25212_length_2774_cov_3.835601"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PKI33_08815"
     CDS             complement(<1..811)
                     /locus_tag="PKI33_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM37151.1"
                     /translation="MSSVISISKLAKHYQVPEREAGLKAAAKGLFKRTYKTVKAVDGI
                     SFDIQPGEVVGFLGPNGAGKTTTLKMLSGLLHPTSGSLSVLGYEPAKRNHDYLRQITL
                     VMGNRNQLSWDLPALDSFDLQRAIYGIPTDEFKRTRDEFIELLDLKELVQKPVRNLSL
                     GERMKMEIVGALLHKPKILFLDEPTLGLDVTMQKRIRSFIAEYNKRYGATVLLTSHYM
                     ADVEALCKRVIVIHHGQLLFDGDLAALVQKFSAFKTVAFDIDNPNHDFSQYG"
     gene            1071..1574
                     /locus_tag="PKI33_08820"
     CDS             1071..1574
                     /locus_tag="PKI33_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM37152.1"
                     /translation="MDKKEIEFQLEDTQRRFIELVNSIPEENYSLPTDNPAWSVGDIL
                     FHITLGPRALALEIWMTAHASGFFGFMMRHFPSRLFNAINAWFGRGRSQRVSRQSLLK
                     AYGKAHAVIMSELRRTREEDLVKSVVYPPEFVSDLAGEVTPERLFRYVTGHFEAHEGQ
                     LRRDSLV"
     gene            1661..1933
                     /gene="rpsO"
                     /locus_tag="PKI33_08825"
     CDS             1661..1933
                     /gene="rpsO"
                     /locus_tag="PKI33_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNM37153.1"
                     /translation="MPLAKEVKTKAIEDFHRHEKDTGSPEVQVAILTNRITQLTEHLR
                     NNKQDQSCRRGLLKLVGQRRRLLTYLRQSNYQSYLSVTERLNLRRK"
     gene            2114..>2774
                     /locus_tag="PKI33_08830"
     CDS             2114..>2774
                     /locus_tag="PKI33_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNM37154.1"
                     /translation="MKPEGKRYSTVVGNKTVTIETGRLAGQAGGALTLGIDEAIVFAS
                     ATMGGVRDGIDFFPLSVEYEERLYAGGKIPGSFFRREGRASTESILTARLTDRPLRPL
                     FQDGMRNEVQVIMYSFSSDGVNPLDILAINAASAAIMISDIPWGGPVGAVRVGRVNGE
                     FVINPTFAEIEASDLDLRIAGTKDAILMVECGANEIPEDVMVQALELGHASIQPIIDL
                     QL"
BASE COUNT          711 a          703 c          697 g          663 t
ORIGIN      
        1 caccatattg actgaaatca tggttggggt tgtcaatatc aaaggcaacc gtcttgaaag
       61 cggagaactt ttgcaccaat gctgcaagat caccatcgaa gagcaactgt ccatggtgaa
      121 tgacaatgac acgcttgcat agtgcttcca catccgccat gtagtgactg gtgagcagca
      181 ccgtcgcgcc gtagcgcttg ttatattcag caatgaaaga acggatgcgc ttctgcatgg
      241 tcacatcaag acctagcgtc ggctcatcga ggaaaaggat tttcggctta tgcagcaatg
      301 caccgacaat ctccatcttc atgcgctcac ctaaagagag attgcgaaca ggcttctgta
      361 ccaattcttt caagtctaac aactcgatga attcatcacg agtgcgtttg aactcatcag
      421 ttggaatgcc ataaatggca cgctgcaaat caaatgaatc aagggcaggc aaatcccaag
      481 agagttgatt gcggtttccc atcactaacg tgatttggcg taaataatcg tggttgcgtt
      541 tagcaggctc ataccccaac accgataaac ttccagatgt ggggtgcaaa agtccgctga
      601 gcattttgag tgtggtggtt tttcctgccc cattcggacc gagaaaaccg accacttctc
      661 cgggttgaat atcaaaagag atgccatcta cagctttgac ggtcttgtag gtgcgtttga
      721 agagcccttt cgcggcggct tttaagcccg cttcacgttc ggggacttgg taatgttttg
      781 ccagtttcga gatcgaaatg acagacgaca tagatttctc ctgaatatgg tagcttaatg
      841 ttcggtatca atagtccgcc ctatggcaat aaaaaaaccg cagggcatca gcgcgcctgc
      901 ggcaaggttt caaaatggga atgaattacc caacccaccc gcggggaggc acgcatagat
      961 ttgttgtttc atgattaatg atagtcattc aagaagtacc tcctttcaga ttagaattag
     1021 ataagcaaag tgtatcacag ccaaaaatca aatgcaagat ggaaagcaaa atggataaaa
     1081 aagagattga atttcaatta gaagatacgc aacgtcgatt tattgaactg gttaattcga
     1141 tacctgaaga aaactattct ctccctacag acaacccagc ctggagtgtg ggagatattc
     1201 tctttcacat taccttgggt ccgcgggcat tggcgctgga aatttggatg acagcacacg
     1261 cctcaggatt ctttggcttt atgatgcggc atttcccttc acggctcttt aacgcgatca
     1321 atgcctggtt cgggcgcgga cggagccaac gcgtcagccg tcagagtttg ctcaaggctt
     1381 atgggaaggc acatgcagtc ataatgtctg aattgaggcg aacgcgggag gaggatttgg
     1441 tcaaatcggt ggtgtatccg cctgagtttg tgtcagacct ggctggagag gtcacgccag
     1501 agcgtctttt tcggtatgtg acagggcatt tcgaggcgca tgaggggcaa ttacggcggg
     1561 attctctggt ataatccatc cccgtgactg gtcctaccga cggtttaaaa gctttcggca
     1621 cggagacctg caatattatt agaaaggaga cgcatacgtc atgcccctcg caaaagaagt
     1681 caagacaaag gcgattgaag attttcaccg ccacgagaaa gacaccggct cacctgaagt
     1741 tcaggtcgca attttgacca accgcattac tcagttgacc gaacacttgc gcaacaacaa
     1801 gcaggatcag tcctgccgcc gtggtttgct caagttggtg ggtcagcgcc gccgcttact
     1861 cacctacctg cgccagagca actatcagtc gtacctcagt gtcactgaac gtttgaactt
     1921 gcgccgcaag taaatagaaa gaaaccccgt aggggcacag tgaacctgtg cccctacatt
     1981 gtgtaaaccc gccgtcagga attgaatagc gcgaataact ctgttgttcc cactcttcag
     2041 tccctgaccc cgaggcggga gcttttcagg ggcgaccgtg actcgcccaa aagattttag
     2101 gagacaatta aatatgaaac ccgaaggtaa acgttactcg accgtcgttg gcaacaaaac
     2161 ggtaaccatc gaaacgggtc gcttagccgg gcaggctggc ggcgctctta cgcttggaat
     2221 cgacgaagcc attgtgttcg cctcagccac catgggcggc gtgcgcgacg gaatcgactt
     2281 cttccccttg agcgtggaat acgaagaaag attgtatgct ggaggaaaaa ttcccggctc
     2341 tttcttccgc cgagaagggc gcgcctctac cgagtcgatc ctcaccgctc gcctcactga
     2401 ccgacctcta cgcccgctct ttcaggatgg catgcgcaat gaagtgcagg tcatcatgta
     2461 ttccttctcg tctgacggcg tgaaccctct cgacatcctt gccatcaatg ccgcttcggc
     2521 tgcgatcatg atctcagaca ttccctgggg cggtcctgtc ggcgccgtcc gtgtgggacg
     2581 tgtgaacggc gaattcgtca tcaacccgac tttcgccgaa atcgaagcct cagacctcga
     2641 cctgcgcatt gccggtacaa aagacgccat cctcatggtc gaatgtggcg cgaacgaaat
     2701 ccccgaagat gtgatggtgc aggctctcga attgggtcat gcttccattc aacctatcat
     2761 tgacttgcag ttgc
//
LOCUS       DAOREF010000388         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25230_length_2773_cov_4.384106, whole genome shotgun sequence.
ACCESSION   DAOREF010000388 DAOREF010000000
VERSION     DAOREF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25230_length_2773_cov_4.384106"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..>2773
                     /locus_tag="PKI33_08835"
     CDS             378..>2773
                     /locus_tag="PKI33_08835"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37155.1"
                     /translation="MSGYEKAIVKDGFLQASPNPIPIGSNLWFEWLSNAKKFSYKYPN
                     GAFVAQHEIRRNTSYWYAYKRRNGKLYKAYLGKTTELTLERLQQIGNFLRGDGVGDPP
                     PLSALTTDSHFFESRIDSSFLPITKITVPVLPRQLVSRGRLNQKISTPLTLIHAPSGF
                     GKSTLLNDWKQTCGYPIAWLTLDEHDNIPARFLYSVVTALQTIHSDFGTDLLTYISAA
                     TSINPAEAVNHLTKGILELQDFHPRLGIVLDDFHRINHSIIYDTLQVWLEHFPSNLQL
                     VISGHTRPPLSLGHLRAQGMATELDVNDLRFTNTEGVKYLQQYVQEPSLAYDDLEKLV
                     KHTEGWAAGLTLTALALSKQSDQRQFIDTFSGAHIYMREYFMETVLQRSNSEIQSFLL
                     RTAVLKNLTGSLCNAVTERTNSDEILEHLWQENLFIVRLEEQGWYRYHDLFAEMLCSQ
                     LQARYPNEVMNLHQRAAQWYREQYAPADAIFHLLSIEAWEEAATLIEEMALRELEQFG
                     EDSRLLRWLQDLPENVVQKHKNLLFVYMRLANNALSQKNIEIFINHIERIISNKPEIL
                     RTPDEKDVLAEIQEIKYTWAQGNTFVPPARSENDYDARWDLLNGLFLLKPAYSQCQEY
                     PEEPIINLLNAAQAQNNLFVTLMTVGSLTKRFVFAGQLRRAEKMCKQALEKALAQRGR
                     LPETASIALNVLGHVSFERNEMDLAQKYLSQSLDTDPNPTSSNMPIQNAILRSKIQVK
                     LGQFDEAIATIQTARNLHVRRPAGVWSSEDLLSYEIYIHLRNNKLDKAEELLHEGPTG
                     T"
BASE COUNT          858 a          624 c          548 g          743 t
ORIGIN      
        1 aaagaaacaa cgtacaaaat tttcttcatg gtgagttttt ctcctcaaat tagggattga
       61 aataaccgat cgaaactcta tcattcatga attaattaca ggcggcttcc acctccttta
      121 cagtcgttat attatttaat tgttatatca gcaatatata tttgcgtgct ctgaaaaagc
      181 actttaaaac atacttcact aacgttataa gaatagttca ggtgcataaa caaattaatc
      241 gggttttcgg atgattttcc acgagagaaa atctataaat tcgtggtcaa aactacgtta
      301 acaaaagatt attaagcaaa aagttaccca acaggtataa ttagaagcgt tgggtaactt
      361 tttacactgg agatagtatg tctggctacg aaaaggcaat tgtgaaagat ggtttcctac
      421 aagcaagccc aaaccccatt ccaattgggt caaatttgtg gtttgagtgg ctttccaacg
      481 cgaaaaaatt ctcctataag tacccaaatg gagcatttgt agcacagcat gaaatccgcc
      541 gaaatacgtc ctactggtac gcctacaaac ggcgaaatgg caaactgtat aaggcatatc
      601 taggaaaaac gacagaactt acccttgaaa gattgcagca aattggaaac ttcttaaggg
      661 gtgatggggt tggtgatccc cccccactga gtgccctcac tactgattcc cacttttttg
      721 agtcgcgcat tgattcgtca tttcttccga tcacaaaaat caccgttcct gttctgccta
      781 ggcagttggt ctcacgcgga cgccttaacc agaaaatcag tacgccacta accttaatcc
      841 atgcgcccag cgggtttgga aaaagcacac ttctcaatga ctggaagcaa acctgtggct
      901 accccattgc atggctaaca ctagatgaac atgataatat ccctgcccgt tttttatact
      961 cagtggtcac agctttgcaa accattcatt cagatttcgg tacagaccta cttacatata
     1021 ttagtgcggc aacttccatc aaccctgctg aagctgtgaa ccacctaaca aaagggattc
     1081 tcgagcttca agactttcac ccacgccttg gcattgtttt ggatgacttt catcgcatca
     1141 atcactctat tatttatgac acgcttcaag tctggcttga acactttcca tcgaatctgc
     1201 aactagtgat ttcagggcat acccgtccgc cattatcgtt aggtcattta cgtgcccaag
     1261 gcatggcaac tgaattggat gtcaacgacc tacgttttac aaacaccgaa ggggttaaat
     1321 acttgcaaca atatgtgcaa gaaccttctc tagcctatga tgatcttgaa aaactagtta
     1381 aacatacaga aggctgggct gctggtttaa ccctaacagc attggcatta agcaaacaaa
     1441 gtgaccaacg tcaatttatt gatacgttta gcggggcgca catttatatg cgtgaatatt
     1501 tcatggagac cgttctccag cggtcaaact cagaaatcca atcctttttg ttaagaacag
     1561 ccgtcctcaa aaatctgaca ggtagtttat gcaatgcagt gactgaacgg acgaactccg
     1621 atgaaattct tgaacattta tggcaggaga atctatttat cgtccgtctg gaagaacaag
     1681 gttggtatcg ataccatgat ctttttgcag aaatgctatg cagccaatta caagcccggt
     1741 acccgaacga ggttatgaat ctccatcaga gagcggcgca atggtatcgt gaacaatatg
     1801 ccccagcgga tgctatcttc cacctacttt ccatagaggc atgggaagaa gccgccacat
     1861 tgatcgaaga aatggcatta cgtgagttag aacaatttgg ggaagactcg cgtctattac
     1921 gttggttaca agacttgcca gaaaatgtgg ttcagaaaca caaaaactta ttatttgttt
     1981 atatgaggtt agccaataat gccttatcgc aaaagaatat tgagatattt atcaaccaca
     2041 tagaacgtat tatttccaat aagcctgaaa ttcttcgcac ccctgatgaa aaagatgtgt
     2101 tggcagagat acaagaaatt aagtacacat gggcacaggg aaataccttt gtgccccccg
     2161 ccaggtctga aaatgactac gatgccagat gggacctact caatggtttg ttccttctca
     2221 aaccagcata tagccagtgt caggaatatc ctgaagaacc gatcataaat ttattaaatg
     2281 ccgcacaagc gcaaaataat ctttttgtca ctttaatgac ggttggttca ctcaccaaac
     2341 gatttgtgtt tgccgggcaa ttgagacgtg ccgaaaaaat gtgcaaacag gctttggaaa
     2401 aggcgctcgc ccaacgaggc agattgccag aaaccgccag tattgcatta aacgtccttg
     2461 gacatgtcag ttttgaacgc aatgaaatgg acttggcaca gaaatatcta tcgcagtctt
     2521 tagataccga ccccaacccc actagcagta atatgcctat tcaaaacgcc attctgcgtt
     2581 ctaagataca ggtcaaatta gggcaatttg acgaggcaat tgccaccata caaactgccc
     2641 gcaacttaca tgttcgccgc ccagctggtg tgtggagcag tgaagattta ctctcttacg
     2701 aaatttacat tcatctccgc aataacaaat tggataaagc cgaagaactt cttcacgaag
     2761 gacccactgg aac
//
LOCUS       DAOREF010000389         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25387_length_2764_cov_5.424880, whole genome shotgun sequence.
ACCESSION   DAOREF010000389 DAOREF010000000
VERSION     DAOREF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25387_length_2764_cov_5.424880"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2764
                     /locus_tag="PKI33_08840"
     CDS             <1..>2764
                     /locus_tag="PKI33_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM37156.1"
                     /translation="ANYRNLVESSESAIAVVDKNGKILFVNLTGNDIWKDRNLVGKSI
                     YDLFPPEYAERYSQAIHSVINAQIGTLDEMQSVIAGKLMWFRLSMTPLKNPDGSISTL
                     LINAWDITDRKVAEQSLRESEEKYRTLIDATSDGVFVAQDERFVFCNKSLPAMLGYTY
                     QEFENIPFERVVLKEHLDVWIDRFRQRIHGESVVSNYQVQFQNKTGVEHIWVELRANK
                     IEYGGRPAVLGIVRDITEQKNILTEIENLAKFPAENPGPVLRIDMYGKLLYANDASFD
                     LLNEWNLEIGKQAPAIIQKVAAEVVETEIDKTIDTTHNGRVVTLNFILLRQKGYINIY
                     GRDITEQRYAEKEVHALQEFSQAIIDALSSHLCVLDERGVIISVNQAWRKFAEENPPI
                     PLNYGIGENYLTICENAKGDYENDALVVARELRAVLEGQKSEAYIEYPCHSPEEDFWF
                     YLSIRRFESKGSTRLLLLHENITHRKQMETEILNVNQRFTELAENVSDIFWVSDPVTR
                     RNVYVNPAFSKVTGMSMDAVENLPNDFLDIVLPEDRNTIIKNRELEESGFKTDSKYRI
                     RRPDGMIRWIHDRATPVIDSSGRVIRIVGISTDITERVDAERRLSESEIRLRQIAEAM
                     PEAFWMVDDKTKKFVYMSPAYKTIWKRSSEELLDGGNQAFMDTVLPEDRAGLSASFIR
                     QEHGEPTEMEYRIVRPDGSLRWIYDRSFPIFDEETGRLLNTAGVAADITEQKLAGEAL
                     KDSEERLRLSLAAAHQGLYDLNIQTGDAVVNREYVEMLGYDFETFKETFQNWGDRLHP
                     EDREIALHAFSEYVNGNSPEYQVEFRQKTKDGSWKWILSMGKIVEYDAMGKPLRMLGT
                     HTNIDANKQAEFELKRRAAQLALINDVGRKIAGVLDIHGVLDLSARLVQEYFDYHHVG
                     LFTLDET"
BASE COUNT          802 a          480 c          701 g          781 t
ORIGIN      
        1 gcaaattacc gtaatttggt tgagagctct gaaagtgcca ttgcagttgt agataaaaat
       61 ggcaagatct tattcgttaa tttaacaggg aatgatattt ggaaagacag gaatttggtt
      121 ggtaaatcta tttacgacct gtttccgccg gaatatgctg aacgatattc acaggctatc
      181 cattcagtaa tcaatgctca gatcgggaca ttggatgaaa tgcagagtgt tattgctgga
      241 aagctgatgt ggtttcggtt gagcatgaca cccctcaaaa acccagatgg ctcgatctca
      301 acattactta tcaatgcatg ggatatcacc gaccgcaaag tagcggaaca atctttgcgg
      361 gaaagtgaag aaaagtatcg aactctgatt gatgctacat ctgatggcgt tttcgtcgct
      421 caggatgaaa ggtttgtctt ttgtaataag tctctaccgg ctatgctggg ctatacctac
      481 caagagtttg aaaatattcc gttcgaacga gttgttctta aagaacattt agatgtgtgg
      541 atcgatcgat ttcgtcagcg tattcatggg gaatctgtgg tttcgaatta tcaggttcag
      601 ttccaaaata aaacaggcgt agaacacatt tgggtagagt tacgggcgaa taagattgag
      661 tatggtggta gaccagcggt tttaggcatt gttcgtgata ttacggagca gaagaatatt
      721 cttacggaaa ttgagaattt agctaaattt ccggctgaga accctggacc tgtacttcgt
      781 attgatatgt acggtaaatt gttgtatgcc aatgatgcta gttttgactt attgaatgaa
      841 tggaatcttg agatcggtaa acaggcgcca gctatcattc aaaaagtagc ggcagaagtt
      901 gtggaaactg agatcgataa aacaattgac actactcata atggacgtgt agtgacattg
      961 aactttatcc ttttgagaca aaaaggttac atcaatatct atggacggga tatcacggaa
     1021 cagaggtatg cagaaaaaga agtgcatgca ttacaagaat tcagccaagc aatcattgat
     1081 gcgctgtcta gccatttgtg tgtgcttgat gaacgtgggg tcattatttc ggtcaaccag
     1141 gcttggcgta agtttgcaga agagaatcct ccaattccgc tgaactatgg gatcggtgaa
     1201 aactatctga caatttgtga aaatgctaaa ggggattatg agaatgatgc gcttgtagta
     1261 gctcgtgaat tgcgcgcagt tttagaaggg cagaagagcg aagcctatat tgaatatcca
     1321 tgccattcac cagaggaaga tttttggttc tatttgagca tacgccgatt tgaaagcaaa
     1381 ggttcaactc ggctgcttct tttgcatgag aatattactc accgtaaaca gatggaaact
     1441 gaaattctga atgtcaatca gcgttttact gaattggcag aaaatgtatc tgatatcttt
     1501 tgggtttcgg atcctgtgac aagacgtaat gtttatgtca acccggcatt ttccaaggtg
     1561 actgggatga gtatggacgc ggttgaaaac cttccaaatg actttttgga tattgttttg
     1621 ccagaagata gaaacacgat tattaagaat cgtgagttgg aagagtcagg gttcaaaaca
     1681 gactcgaagt atagaatccg ccgtcccgat ggaatgatcc gttggatcca tgatagggca
     1741 actccggtga ttgattcgtc tggtagggtc atccgtattg tcggcatttc aacggatatc
     1801 actgaaagag tagatgcaga gcgacgtttg agtgagtcgg aaatacggtt gcgccaaatt
     1861 gcagaagcca tgccagaagc gttttggatg gttgacgata agacaaagaa gttcgtttac
     1921 atgagccctg cttataaaac catttggaaa cgttcttcag aggaattact ggatgggggg
     1981 aatcaggctt ttatggatac ggtcttgcct gaagatcggg ctggcttaag cgcatccttc
     2041 ataagacagg aacatggtga gcccactgaa atggaatatc gaatcgtcag acctgatggt
     2101 tcgttgcggt ggatctatga ccggtctttc ccgatctttg atgaggaaac aggtagatta
     2161 ttgaataccg ctggtgtggc agctgatatt acagaacaga aattagcagg tgaagctctg
     2221 aaagatagtg aagaaaggtt gcgcctttca cttgcggctg ctcaccaagg tctttatgac
     2281 cttaatattc agaccgggga tgcggttgtt aaccgcgagt atgttgaaat gcttgggtat
     2341 gattttgaaa cctttaaaga gactttccaa aattggggag accgtttgca tcctgaggat
     2401 agagagattg cccttcatgc gttctctgaa tatgtcaatg gaaactcccc ggaataccaa
     2461 gttgaatttc gtcagaagac gaaagatgga agctggaagt ggatcctttc catgggcaag
     2521 atcgttgaat atgatgccat gggaaaaccg cttcgtatgc ttggaacgca tacgaatatt
     2581 gatgcgaata aacaggcaga atttgagcta aaacgccgag cggctcaatt ggcgttgatc
     2641 aatgatgtgg gtagaaaaat tgctggggtg ctcgatattc acggcgtatt agacctttct
     2701 gctcgtcttg tgcaggaata ttttgactat caccatgttg gcttgtttac gttggatgaa
     2761 acga
//
LOCUS       DAOREF010000390         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25457_length_2760_cov_4.677634, whole genome shotgun sequence.
ACCESSION   DAOREF010000390 DAOREF010000000
VERSION     DAOREF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25457_length_2760_cov_4.677634"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2760)
                     /locus_tag="PKI33_08845"
     CDS             complement(<1..2760)
                     /locus_tag="PKI33_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM37157.1"
                     /translation="MPERFRDTHAKQKKAFLKAPQKRAMGSGLDIYGLHQSGKEIPVE
                     VGLNPVTIDGEKYVLVSVSDISTRKEMEVALAASRLKESEEKFRTLVEQNPAIVYISG
                     LHQHIGVTYISPQIQTLGFTQAEWIADPNLWVEQIHPEDQKRVLKEVEEFALKNQPFK
                     SEYRLLAKDGHVRWFLDEAVDIIDSNGSAIIRQGFMLDISARKDAEETLSSREHYLAL
                     LNAITTSIIASQDMDGSIESLVEGLKELLSADDCYMTRWDEETEKPAPWGSTAEMEQP
                     YMTMTFPDGLRSMSSSVIEEGHALVAEDVFNSPYVSPEIAKMFPAHSMLGIPLLYRNK
                     KLGAIIVAYNTLHKFDTGEIERAEQAGRQIAIAMWSVQQDIALKKRLKEQETLAQITT
                     TLSQVEHIGLSNVLDLIVHAARELIPEAQQAVIHLMDRGNAYLTPQAVSGYEQTGESG
                     GKMRVGEGAAGLAMSEGRSVYIPSIHTDERFIHLSSQVKYNSLLVTPIISSSQKLGTI
                     SIQSEKAFAFSKSAINLLNELGQQAAIAIENTRLYDAAQQELKERFLAEAALRSSEER
                     YRSVSEDIPAMICRFDRGGTLTYVNQAYADFFGGTPSELINKSFFDMLDEEQKDLVRS
                     NFYSLTPDKPFIVYEIHEKNCKGEKRWVQWTDRLITNENDIKAEYQSIGMDITDRKLA
                     EIEREHLLEAEHDQRLRAETSAEATLALVSHVELDKVLIEILNQVQRLLPGCGANIAL
                     LENNILRTAAWRGYENRGEEVFKNLIKDTGMYPLDQTIVKNPKTSIIADTALDEDWII
                     IPGLEWIRSHLCIPLLWNKQLLGLLYIDENTPNKFSEETIHRLTPLVNATTVALESAL
                     LIETTRQALKETSALYHINKGMVALDPDELLNDAVNLLKNNFDYYHVQVYVPNPATGN
                     FYTKAA"
BASE COUNT          693 a          621 c          597 g          849 t
ORIGIN      
        1 tgcagctttt gtataaaagt ttcccgttgc agggttgggc acatacacct gaacgtggta
       61 ataatcaaag ttatttttta agaggtttac ggcatcattt agcaactcat caggatcaag
      121 tgctaccata cctttattga tgtgatacaa agcacttgtc tcttttagtg cctgacgggt
      181 cgtttcgata agtaaagcgc tttcaagcgc aaccgtggtt gcattgacca aaggcgtaag
      241 acggtgaatc gtctcctctg aaaacttatt cggcgtattt tcatcgatgt ataacaaacc
      301 taataattgt ttattccaca acagcggaat acaaagatga gaccgaatcc attccagccc
      361 aggaatgatt atccaatctt catccaaagc tgtatcggca atgatcgatg tctttggatt
      421 cttgacaatg gtttgatcca aaggatacat gcctgtatct ttgattaagt ttttgaatac
      481 ttcttcgcct cggttttcgt agccgcgcca tgctgcagtt ctcagtatgt tattttctag
      541 tagggcaata tttgccccac agcccggcag aagcctttgt acctggttca aaatctcaat
      601 tagaacttta tctaactcta catgcgatac gagagctaat gttgcttcgg cgctggtttc
      661 ggcgcgtaag cgttggtcat gctctgcttc taaaaggtgt tcgcgctcga tttctgccag
      721 ctttcggtca gtaatatcca taccgatcga ctgatattcg gctttgatgt cattttcgtt
      781 tgtaattaat cggtctgtcc actgcaccca gcgtttttca cctttgcaat ttttctcgtg
      841 gatctcatac actataaaag gtttatcagg tgtgagagaa taaaaattgg atcggacaag
      901 atctttttgt tcttcgtcta acatatcgaa gaaactcttg ttgatgagtt cacttggtgt
      961 gccaccgaag aaatctgcat aggcttggtt gacataggtc agcgtaccac ctcggtcaaa
     1021 gcgacagatc atcgcaggga tatcttcaga aacactgcgg tagcgttctt cactgctgcg
     1081 taaggctgct tctgcaagga aacgttcttt tagttcctgt tgggcagcat cgtaaagcct
     1141 agtattttcg atcgcgatgg cggcttgttg cccaagttcg ttgagcaagt tgatggcgct
     1201 tttagagaaa gcaaatgcct tctcgctctg tatgctaata gtgccaagct tttggctact
     1261 gctaataatt ggcgtaacta aaagagagtt gtatttcact tgcgagctta agtgaataaa
     1321 gcgctcatca gtatggatcg aaggaatgta aacgcttctg ccctcagaca tggcaagccc
     1381 ggcagcacct tcacccacgc gcatcttgcc tccgctttcg ccggtttgtt catacccaga
     1441 aacagcttgt ggggtaaggt aggcattgcc tctatccatg aggtgaatca cagcttgttg
     1501 cgcttcagga attaattccc ttgcggcatg aacgatcaag tcaagcacgt ttgataaacc
     1561 aatatgctcc acctggctaa gtgtggtggt gatctgggca agggtttctt gctctttcaa
     1621 gcgtttctta agtgcaatat cttgttgaac actccacata gcaatagcaa tttgcctgcc
     1681 agcttgttct gctctttcaa tttcgcctgt gtcaaatttg tgcaaggtat tgtaggcaac
     1741 aatgatcgcg ccaagttttt tatttctgta tagaagtgga attccgagca tggaatgtgc
     1801 cgggaacatt tttgcaattt ctgggctgac gtaaggagaa ttaaagacat cttctgcgac
     1861 aagtgcgtga ccttcttcaa tgactgagct gctcattgag cgtaatccgt cagggaaagt
     1921 cattgtcata tacggctgtt ccatctctgc cgtagagccc cagggcgcag gtttttcggt
     1981 ctcttcatcc caacgagtca tataacaatc gtcagcactg agaagttctt ttaacccttc
     2041 aactagactc tcaatggagc catccatatc ttgggatgca atgatcgaag tagtaatggc
     2101 attcaataaa gcaagataat gttcccgaga agataaggtc tcttcggcat cttttctggc
     2161 gctaatatcc agcataaatc cttggcggat aatggccgat ccgttactat caatgatatc
     2221 tactgcttca tctaagaacc aacgtacatg cccgtctttt gctagtagtc tgtattctga
     2281 cttgaatggc tgatttttta aggcaaactc ttcgacttct ttcaacactc ttttctgatc
     2341 ttcaggatga atttgttcta cccacaaatt tgggtcggca atccattctg cttgagtgaa
     2401 acccaacgtt tgtatctggg gactgatata agtgaccccg atatgctggt gaagacccga
     2461 aatataaaca atagccgggt tttgttctac gagtgtacgg aatttttctt cagactcttt
     2521 taatcggctg gctgccaggg caacttccat ctcttttctt gtagaaatat cagagacaga
     2581 aaccagaaca tacttttcgc cgtcaatagt gaccggatta agaccaacct caactggaat
     2641 ttccttgccg ctttgatgca gaccatagat atccaaccct gaccccattg ccctcttttg
     2701 aggagctttg agaaaagctt tcttctgttt cgcgtgcgta tcccgaaacc tttcggggat
//
LOCUS       DAOREF010000391         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25477_length_2759_cov_3.937870, whole genome shotgun sequence.
ACCESSION   DAOREF010000391 DAOREF010000000
VERSION     DAOREF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25477_length_2759_cov_3.937870"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKI33_08850"
     CDS             <1..463
                     /locus_tag="PKI33_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37158.1"
                     /translation="AFQKDIDSQTRISRRHVTFLILYTSAILFLWFVAVKMFNAAWAY
                     SFFIGMLVLMEVTIHMRHFRNWYFLKVFRSKGGITGGITYAQWFSYRVSAFEFYCFAF
                     LYLFFFLLTYSFFFLGGVVICLRIGISHSNLAKKVERQRAVSVTKNENSSS"
     gene            444..1094
                     /locus_tag="PKI33_08855"
     CDS             444..1094
                     /locus_tag="PKI33_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNM37159.1"
                     /translation="MKILHRDSSIVVVDKPAELSVLAEGWNKEMPYLAKMLEEQFGKV
                     WVVHRLDKGTSGVMVFALTAEAHRSLNIQFEKHEVEKAYHALVNGIPKWDEKVTKFPL
                     RVNVGHKHRTVVDDRNGVPAETRFRLLERYQAAALVEASPMTGRTHQIRVHAYALGYP
                     LVGDILYSAPETNLIARPALHARSLTLTHPENGKRLTFQADYPNDLLNAINLLRGK"
     gene            1137..1814
                     /locus_tag="PKI33_08860"
     CDS             1137..1814
                     /locus_tag="PKI33_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37160.1"
                     /translation="MNERILIIEDDQAILKLLQRGLAYEGYTVDTATDGRMGLIAARD
                     HTPDLVVLDWMLPGMDGLEVCHRLRQGGSIPILMLTAKDTVQDRIQGLDAGADDYMVK
                     PFNLDELLARVRALLRRTQPERIPVLKFADLSLDTGSRQATRGNRTVALTAKEYELLE
                     LFLRHPKQVLTREVIFDRVWGYDFGGESNVLEVYIRYLRQKLEAENEQRLIHTVRGVG
                     YVLRENP"
     gene            1931..>2759
                     /locus_tag="PKI33_08865"
     CDS             1931..>2759
                     /locus_tag="PKI33_08865"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNM37161.1"
                     /translation="MSLRLRLTLLYSTFMGGILLVFGAAVYILVNVILLNQVDNLLAN
                     VAREIVRATTVDSRGDLNVISLPTLDMTSNAYVQVWGPDGKLITTSPSIGSLTKALDP
                     VGLESNITMYEDSYLDEAHLRVLTVPLKLRSRIIGTLQVAASLTVVDATRTSLLSIMA
                     IIALVAVGLVMWGAWGVLGRALSPLQDIADTVDQINRADDLSRRIPTESAMRDDVGDL
                     VISINQTLERLESLFTSQQRFLADVSHELRTPLTVIKGNVDLIRKFKEADEDLLNSID
                     "
BASE COUNT          646 a          582 c          744 g          787 t
ORIGIN      
        1 ggcatttcaa aaagatattg atagccaaac cagaatttca aggcggcacg ttacgttctt
       61 gattttgtat acatccgcca ttctttttct gtggtttgtt gctgtaaaaa tgttcaatgc
      121 ggcttgggca tattcctttt ttatcggtat gcttgttctc atggaggtga ctatccatat
      181 gcgtcacttc cgcaattggt actttttgaa ggtgtttcgc agcaaaggcg gaatcacagg
      241 cggcattacc tatgctcagt ggttctcgta tcgggtgtct gcttttgagt tttattgctt
      301 tgccttctta tatttgttct tcttcctgct tacctacagc tttttctttt taggcggggt
      361 cgtgatttgt ttacgaattg gaatatctca ttccaacctt gcaaagaaag ttgaacgcca
      421 acgcgctgtt tctgtaacta aaaatgaaaa ttcttcatcg tgattcgtct attgtagtgg
      481 ttgataagcc tgccgaactc tctgtccttg cagaggggtg gaataaggaa atgccttatc
      541 ttgcgaagat gctggaagaa caattcggca aagtatgggt ggtgcatcgt ttggataaag
      601 gcacaagcgg agtgatggtc tttgcactga cggcggaggc tcatcggtcg ctgaatattc
      661 agtttgagaa gcatgaagtg gaaaaagcct atcatgcctt ggtgaacggt attccaaaat
      721 gggatgagaa ggtgacgaaa tttccattgc gtgtgaatgt ggggcataaa catcgcactg
      781 tggtggatga tcgcaatggc gtcccggctg agacgaggtt taggctgctg gaacgctatc
      841 aagctgcggc gttggtagag gcgtcgccaa tgacggggag aacgcatcag attcgtgtcc
      901 atgcctatgc gttgggttat ccactggtgg gggatatcct ttatagcgca ccggaaacta
      961 acttgatcgc gcgcccggca ttgcatgcac gatcgttgac attaacacat cctgagaatg
     1021 gtaagaggtt gacctttcaa gcggactatc ctaatgattt attgaatgct ataaatttgc
     1081 tgagaggcaa ataagcctct tgaatttatg gtatattttt agtaaggagt atagccatga
     1141 acgagcgaat cctcatcatc gaagacgatc aagctatctt aaagttgtta cagcgcggtc
     1201 tggcgtatga aggttatacg gtggataccg ccacagatgg acgcatggga ttgatcgcgg
     1261 ctcgcgacca tacccccgat ctggtcgtcc tggattggat gttacccggc atggatggtt
     1321 tggaagtgtg ccatcgtttg cgacagggcg gttcaattcc gattttgatg ctgactgcca
     1381 aagataccgt gcaagaccgc attcagggat tggatgccgg tgcggatgat tacatggtca
     1441 aaccgtttaa cctggatgag ttattggcac gtgtgcgtgc gcttttgcgt cgaacccagc
     1501 cagaacgtat tcctgttttg aagtttgcag acttgtcact ggatacaggg tcacgtcagg
     1561 caactcgcgg gaatcgcacc gtcgctttga ccgcgaagga gtacgaattg ctggaattat
     1621 tcctgcgtca ccccaagcag gtgcttactc gcgaagtgat ctttgaccgt gtgtggggct
     1681 acgactttgg cggcgagagc aatgtgttgg aagtgtacat tcgctatctg cgtcaaaagt
     1741 tggaagccga aaatgaacaa cgccttattc ataccgtgcg cggtgtggga tatgtcttaa
     1801 gggaaaatcc gtaatgaaac ggcgggcaac cgccgttttt ctattatggt gagaaagatc
     1861 agggtatgaa atgatatttc tcaccatcta ttcttagctt gccctaaact cggacaatta
     1921 aactgtgacc atgtcattgc gtttgcgcct cacgttgcta tactccactt tcatgggagg
     1981 gattttgctt gtttttggcg cggcggttta tattctcgtc aatgtcattt tgctcaacca
     2041 agtggacaat ttgttggcga atgtggcgcg tgagatcgtc cgtgcaacga cggtcgattc
     2101 acgaggcgac ttaaatgtca ttagcctgcc cacattggat atgacctcga acgcctatgt
     2161 acaggtttgg ggacccgatg gaaagctgat taccacctct ccgagcatcg gttcgttgac
     2221 caaagcgctt gacccggttg ggttagagtc gaatataacg atgtatgaag actcttatct
     2281 cgatgaagcg catctgcgtg tgttgacggt tccgctaaaa ctgaggagcc gcatcattgg
     2341 gactttacaa gtggcagcca gcctgacggt ggtggatgcc actcgaacca gtctgctttc
     2401 aattatggcg attattgctt tggtggcagt ggggctggta atgtgggggg catggggggt
     2461 gcttggacgt gctctttcgc ctttgcaaga tattgccgat accgtagatc agatcaaccg
     2521 tgcggacgat ttatcgcgcc gtattccaac agagagtgcc atgcgtgatg atgtgggcga
     2581 tttggttatc tctattaatc agactctgga acgcctggag tcgttgttta cgtcgcaaca
     2641 gcgttttctt gcagacgtga gccatgaatt acgcaccccg ttgaccgtga tcaaaggcaa
     2701 cgtggatttg atccgcaagt tcaaagaagc cgatgaagat ttgctcaata gcattgacc
//
LOCUS       DAOREF010000392         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25607_length_2752_cov_3.882462, whole genome shotgun sequence.
ACCESSION   DAOREF010000392 DAOREF010000000
VERSION     DAOREF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25607_length_2752_cov_3.882462"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKI33_08870"
     CDS             complement(<1..247)
                     /locus_tag="PKI33_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HNM37162.1"
                     /translation="MRYFVIVNPTSGRGLGEKSIPQIESFLKEHGFDFTLVRTTGVWH
                     AADLAESAARDGYDVIVCASGDGTVNEAINGMMRAKKA"
     gene            complement(282..854)
                     /locus_tag="PKI33_08875"
     CDS             complement(282..854)
                     /locus_tag="PKI33_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNM37163.1"
                     /translation="MKHISAIILAAGKSTRMGRQKMLMPWGSATVLEKVIQTVQAAEI
                     EDIILVASPEIASKFQDKYLQVVLNAEGEMLKSIQLGLQAQKESAEATLICLGDQPQM
                     EEGSVRSVCEAFQKNAANLVVPSYQMRRGHPWLVERSLWSEILSMQNGTMREFLDKHA
                     NQIQYVNLSTPTILQDLDTPEDYLKYKPSM"
     gene            complement(851..2176)
                     /gene="yqeC"
                     /locus_tag="PKI33_08880"
     CDS             complement(851..2176)
                     /gene="yqeC"
                     /locus_tag="PKI33_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNM37164.1"
                     /translation="MDLIHALRLDQAKENPVVCFVGAGGKTTALFQLARQRIGKKNSL
                     VLVTATSHLGVWQIPLADHHVIVNKIEDLQLPSNGVVLVTGEIEKDRTLPVTGNALNW
                     LRAKCNSENVPLLIEADGSRQKPVKAPATHEPPIPDFADIVVYVCGISGIGKALNDEN
                     IHRPEIFSELTNLKMNEQVSAQSLISFLTHPQGGLKNIPAHANRIALLNQADTPELQS
                     MGGAIAQKLLPTFDAVVIGSLQNNVIHLVERTAGIILAAGSSSRYGQLKQLLDWKGKA
                     FVRHVAETALQAGLDPVVVVTGFRHADIAPHLQDLPVNIVYNPAYEQGQSTSIKAGLA
                     ALPKNTGAALFLLADQPQIPADVMRALIESHASERQSILAPLVLEEKRANPVLFDRVT
                     FPDLMKLTGDIGGRGVFDKHKVSFLPWHDDILIFDVDKPEDYERLKNIE"
     gene            2281..>2752
                     /locus_tag="PKI33_08885"
     CDS             2281..>2752
                     /locus_tag="PKI33_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37165.1"
                     /translation="MDKPTALIIEDDRDIVALFRHVLDVAGYQTEIVLDGKDAMDRLD
                     VMRPNIVLLDLQLPRVSGIDILKRMRDEERLMRVPVVVITAYAPYADSLPVEPDLLLL
                     KPVDINQLSNLVQRLQSTQGALDEPTHDFVTGLYTLSFFNVRMTFSLERIKQSSF"
BASE COUNT          681 a          636 c          621 g          814 t
ORIGIN      
        1 cagctttttt agcacgcatc ataccgttga tggcttcatt caccgtgcca tctccgctgg
       61 cacaaacgat cacatcatag ccatctcgcg cggcactttc agccaagtct gctgcatgcc
      121 aaaccccagt tgtgcgcacc agggtgaaat caaagccgtg ctcttttaga aatgattcaa
      181 tctgtggtat ggatttttcc ccaagcccac gccccgaagt cggatttacg atcacaaagt
      241 atctcatgcc tgctcccagt gtgaaaagtg gagggagttt atcacatgga tggtttatat
      301 ttcaagtaat cttcgggtgt atcgaggtcc tgcaaaatcg tgggtgtgct taaattcaca
      361 tactggatct gatttgcatg cttatctaaa aactctcgca tcgttccatt ttgcatgctc
      421 agaatttcac tccataaact tctctctacc aaccacggat gccctctccg catttgatag
      481 cttggcacta caaggtttgc agcatttttt tggaatgctt cgcatacgct tcgcacgctt
      541 ccttcttcca tttggggctg gtcgccaaga caaattaatg ttgcctccgc tgattccttt
      601 tgtgcttgca atccaagctg aatcgatttc agcatttctc cttctgcatt aagcactacc
      661 tgcaaatatt tatcttggaa ctttgatgcg atttcggggc tagccaccaa aataatatct
      721 tcgatttctg ctgcctgaac ggtttggatt actttttcca gtacagtagc gcttccccaa
      781 ggcatgagca ttttctgtcg ccccatacga gtagactttc ctgctgcaag gataatcgcc
      841 gagatatgct tcattctata tttttcaacc gctcataatc ttcgggctta tccacatcga
      901 agatcaaaat atcatcatgc caaggtaaga aggaaacttt gtgcttatca aatacgccgc
      961 gcccgccaat gtcacctgtc aatttcatta agtctgggaa ggtaacgcga tcaaagagaa
     1021 ccgggtttgc gcgtttttcc tccaacacaa gcggagccag aatggactgc ctttcactag
     1081 catgagactc aatcaacgcc cgcatcacat ccgctggaat ttgcggctgg tcggctaaga
     1141 gaaataaagc tgcgcccgtg tttttcggta acgctgcaag acccgctttt atggaagtgc
     1201 tttgtccctg ttcgtatgcg gggttgtaga caatatttac tggcaggtct tgtaggtgag
     1261 gggcaatgtc ggcgtggcga aagcctgtaa cgaccactac agggtccagc ccggcttgaa
     1321 gcgcggtttc tgctacatgt cgaacaaacg ccttgccttt ccaatccaaa agctgtttaa
     1381 gctgaccata tcggctggat gacccggctg caaggataat tcctgctgtt cgttctacga
     1441 gatgtatgac gttattttga agcgagccaa taacaacagc atcaaatgtt ggtaacagtt
     1501 tctgcgctat cgctccaccc atagactgca actctggagt atcggcttga ttaagtagtg
     1561 cgattcgatt tgcatgtgca ggaatatttt tcaacccacc ttgaggatgg gtaaggaatg
     1621 aaataagaga ttgcgctgag acttgctcgt tcattttgag gtttgttagc tctgaaaaga
     1681 tttcagggcg gtgaatgttt tcatcattta gcgctttacc gatgccgctt ataccgcaaa
     1741 cataaaccac gatgtcagcg aaatcaggaa taggcggttc atgcgtggca ggtgctttga
     1801 ccggtttttg acgtgaccca tctgcttcga tgagaagagg gacattctca gaattgcatt
     1861 ttgcgcgtag ccaatttagg gcattgcctg ttactggtaa ggttctgtct ttctctattt
     1921 cacctgtgac tagaacgact ccatttgaag gtaattgtaa atcttctatt ttattcacta
     1981 ttacatgatg gtctgcaagc ggaatttgcc acacacccaa atggctggtg gctgttacaa
     2041 gcacaagcga attctttttt cctattcgtt gacgtgccag ctgaaagagg gcagtggttt
     2101 tgccaccggc acccacaaaa caaacaactg gattttcttt ggcttggtca agacggaggg
     2161 cgtgtattaa gtccatgtgg attagctttc gttgtaattg tatgataaaa aacctgaaat
     2221 gacaccctga acggtgcttg aaggtcgtat aatatctata ttgcgaagaa gggcgaactt
     2281 atggataaac ccactgcttt aattattgaa gatgaccgcg atattgttgc gctatttcgg
     2341 catgtattag atgtcgcagg ctaccagaca gagattgtgc tggatggtaa agacgcaatg
     2401 gaccgcttgg atgttatgcg tccaaatatc gttttattgg atttgcagtt accaagagtt
     2461 tcaggaattg atattctaaa gcgtatgcgt gatgaagaac gtctcatgcg cgtgcctgtg
     2521 gtggtaatta cagcctatgc tccttatgca gatagcctgc ccgtcgagcc agacctgttg
     2581 cttcttaaac cagtcgatat caatcaattg agcaacctgg tacagcgttt gcaatctaca
     2641 caaggcgcgc ttgatgaacc tacgcatgat tttgttactg gcctttatac gttgtctttt
     2701 ttcaatgtgc gtatgacctt ttcactggag cgcatcaagc aaagttcttt tc
//
LOCUS       DAOREF010000393         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25653_length_2749_cov_4.030809, whole genome shotgun sequence.
ACCESSION   DAOREF010000393 DAOREF010000000
VERSION     DAOREF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25653_length_2749_cov_4.030809"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1751
                     /locus_tag="PKI33_08890"
     CDS             135..1751
                     /locus_tag="PKI33_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNM37166.1"
                     /translation="MKKNNYKWGRLFFLIVALLTIFSLLLSACAPAQGLEGGAGGNGG
                     GNGGGNGGGGGNAGGSGNGGGGGGNAGGSGNGGGGNGGGNAGGGSASDPGTGDPSVQN
                     PDGVVVRGSKQDSSDPCLDAGQLPCGKGDHGKGNPVNGSLHANCRAAQGEVDPVCAPK
                     PPKDIIPDGGGNNGNGGGGGGGGTPPVVVDPPILPTPPVVEVTAAPKVKVDDNGVASC
                     PNWIMFHSFRSGNLDIFRLDGAEGVDGVQAINLSQDNSQDSRPSRSPNDEFVVFQSDR
                     DGNVELYLTDSKGNSQSRLTNTKSNNINAMFGPDSKSVIYQSDRNGNWDIFLLNKDTG
                     EELQLTSDAGDDVNPFYSPDNKWITFQSNRTGSWNVFVLDVSTGTEYQVTSFSTDVLF
                     PSWSPNGKQLAFLINMTGTWDLYVSDIQGKGFKQITTGGDAQNATWSPEGDRLAYQVT
                     YNGNTDIFTFDLTTNEEYQLTNFGGVDSAPTWNCGGTDVSFTSNREGDPNIYSTWWQG
                     GGDIYGITVDPSTDKWSQWSPSKEPGSRGY"
     gene            1881..>2749
                     /locus_tag="PKI33_08895"
     CDS             1881..>2749
                     /locus_tag="PKI33_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNM37167.1"
                     /translation="MLVIALLIAFTFPIAFLILLDRFDLYNTGRFHFNLLTLAGGLVA
                     YFFAAQINPWVVNMGWASWGTVVRFVAPVVEEILKSLILIYLVQRADFNYVVDGALYG
                     FGAGIGFAVIENYEYVTGNAQIALVLALVRVFSTNLMHATGSGLIGTALAYSRGDKNK
                     RQGLLIILGGYAVAMLFHGLFNTMVNAGAFLVLAIAYGVIGLWLIWTIIRRGMTVQKD
                     WVAEKLGMADRVTLEETKVVKNIETVNEVLRPVEQRFGAEKASLVRNLIYKQAEIGIK
                     RKLLESVASEARK"
BASE COUNT          679 a          575 c          737 g          758 t
ORIGIN      
        1 cttccggtgg cagtatgtat gtcggtttgg gattgctagc cttgctgttc attgtctatt
       61 tggtttctcg caagaaataa tagtcaaaat aaaaacgcca aaccaatgta tttatttgta
      121 tcaaggagag atcaatgaaa aaaaataatt acaaatgggg cagattgttt tttcttatcg
      181 tcgctttgtt gacgatcttt tcactgcttt tatctgcttg tgcccctgct cagggattag
      241 aaggcggcgc tggtggcaat ggcggcggaa atggtggcgg aaatggtggc gggggcggta
      301 acgctggtgg tagtggaaat ggtggtgggg gcggtggcaa tgccggtggt agtggaaacg
      361 gcggcggtgg caatggtgga ggtaatgctg gcggtggttc agctagtgac cctggaacag
      421 gggatccgtc tgtccaaaac cctgacggcg tcgtggttcg tggatcgaag caagatagct
      481 ctgacccctg tttagatgct ggacaattac cctgcggtaa aggcgatcat ggaaaaggca
      541 accctgtaaa tggaagtctc catgccaatt gccgtgccgc ccaaggcgaa gtggacccgg
      601 tttgtgcacc gaaaccaccc aaagatatta tccctgatgg tggtggaaac aatgggaatg
      661 ggggcggtgg tggcggcggt ggcactccac cagtcgtagt ggaccctcct atattaccaa
      721 ctccacccgt ggttgaagtg acagctgcac caaaagtaaa agttgatgat aatggagttg
      781 catcctgccc gaattggatt atgttccatt cattccgctc tggtaactta gatatcttcc
      841 gtttggatgg cgccgaaggt gttgatggtg ttcaagcgat aaatctaagt caggataata
      901 gccaggactc acgcccgagt cgctctccca atgatgaatt cgttgtgttt caaagtgacc
      961 gcgatgggaa tgtggaactg tacctcactg atagcaaggg caactcacag tctcggctta
     1021 ccaataccaa atccaataac attaatgcca tgttcggacc cgatagcaaa agtgtgatct
     1081 atcagagtga ccgcaatggt aactgggata tcttccttct taataaagat acgggtgaag
     1141 aactgcagtt gacttcagat gctggcgatg atgttaaccc tttctactca ccagataaca
     1201 aatggatcac tttccagtct aaccgaaccg gttcttggaa cgtgtttgtt ctggatgttt
     1261 caacaggtac agaatatcaa gtaacatcat tttctacaga tgtgctcttc ccgagttggt
     1321 cgccaaatgg caagcaattg gcattcttga tcaatatgac cgggacatgg gacctatatg
     1381 tttcagatat tcagggcaaa ggcttcaagc agattacgac tggtggcgat gctcagaacg
     1441 caacctggtc tcctgaaggt gatcgcctgg catatcaggt aacctataac ggcaataccg
     1501 atatctttac ctttgatcta actaccaatg aggaatacca gttaacaaac tttggcggag
     1561 tggactccgc tccaacctgg aattgtggcg gcacggacgt gtcctttact tcgaatcgtg
     1621 aaggtgaccc gaatatctac tccacatggt ggcagggtgg tggcgatatc tacggcatta
     1681 cagtagaccc ctccacagat aagtggtctc agtggtcgcc atccaaagaa ccgggttcgc
     1741 gtggatatta gactgaacat tttgagaaat tccccgccct tgaacttctc aagggcggag
     1801 tttgtttaac aactaaaacc gatacggctt gttaatgatt tttccttgcg ggtcgtttct
     1861 agataaggta tgattttgcc atgcttgtga tcgccttact gatcgctttt accttcccaa
     1921 ttgcgttttt aatccttctt gaccggtttg acctatacaa taccggcagg ttccatttca
     1981 atttacttac cttggctggt ggcttggtgg cgtatttctt tgcggcacag atcaaccctt
     2041 gggttgtgaa tatgggatgg gcatcttggg ggacagtggt ccgatttgtg gctcccgtag
     2101 tggaagaaat attaaaatct ttaattctga tctacctcgt ccaacgagcg gactttaact
     2161 atgtggtgga tggggcactc tatggttttg gggcaggcat cggctttgct gtgatcgaga
     2221 attatgaata tgtcactgga aacgctcaga ttgctctagt gcttgctttg gtgcgtgtct
     2281 tttccacgaa cctcatgcat gctacgggta gcggtctgat tggaactgcg ttggcgtatt
     2341 cgcgtggtga taaaaataaa cgccaaggtc tattaattat cttgggcggc tatgcggttg
     2401 cgatgctctt ccatggactg ttcaatacca tggtcaatgc cggagcgttt ttggttttgg
     2461 cgattgcgta tggagtcatt ggtctatggc tgatctggac gatcattcgc cgcggtatga
     2521 ccgttcaaaa agattgggtg gcagaaaaac tgggtatggc agatcgcgtc actcttgaag
     2581 agacgaaagt ggtcaaaaat attgagacgg taaatgaagt gctccgcccg gtggaacagc
     2641 gttttggagc agagaaagct agtttggtac gtaatcttat ttataaacag gcagagatcg
     2701 gcatcaaacg taaattgctc gagtctgttg cgagtgaagc gcgtaagaa
//
LOCUS       DAOREF010000394         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25730_length_2744_cov_4.383042, whole genome shotgun sequence.
ACCESSION   DAOREF010000394 DAOREF010000000
VERSION     DAOREF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25730_length_2744_cov_4.383042"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1618
                     /gene="tilS"
                     /locus_tag="PKI33_08900"
     CDS             155..1618
                     /gene="tilS"
                     /locus_tag="PKI33_08900"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNM37168.1"
                     /translation="MSSNVETIMVEECGLVKDRPVIVGVSGGPDSLCLMETLRRAGYH
                     LIIGYFDHKLRPESALDARMVEKAAVRLGLLCILGGMDVRSYAEEKKLSIEEAARTLR
                     YRFLFELARKYKAQAVAVGHTADDQVETVLMHFLRGSGISGLKGMSYRSVIKSFDPDI
                     PVVRPLLDMWREETVLFCAVNGLRPSYDSSNESLNFQRNRIRHLLIPSLETYNPKFRE
                     TVLRMSHSLKGDYAFVMETLEHAWGNVVVSANDEIITFDVLRLSSMPIGLQRNLIRHA
                     MQTLLPGVDIGYQTLKRASDFINASSATVPRLDLKAGLRMFREAGLIHICAPYAELPF
                     DRWPQMVDDKSVPLRLGGQASLAGGWKVICESWRLPTLAREQAEENEDQFQVWLDADE
                     LPEEFLLRARRQGDHFSPLGMGGHSQKLSDFFVNEKLPHRARDRWPLLCVGDEIIWVP
                     GFRPAHRYRLKETTHNVIYFSVQHPPEKIAPDENPHP"
     gene            complement(1638..2537)
                     /locus_tag="PKI33_08905"
     CDS             complement(1638..2537)
                     /locus_tag="PKI33_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNM37169.1"
                     /translation="MTKNSAPAHISAKTLLPAAINGWEMFLLDQGRSPNTVKAFLSDV
                     RLLTQFLPPDQAIGTVTTDDLNRFFEWMEKGRGVACSPKTLSRRITSVKSFFRWLHQY
                     GTLSIDPAEKVLQRSAISPLPQVLTDEEYEAVLLAADRQRRAKKPDARPYTLLSLLLQ
                     TAIKKSECLGIHVNHIELAGKNGPFLFVRYASPANRYKERKIELTEDWVEAYNEYLTQ
                     YSPVDQAFPWSPRRLEYLLEDISEEAGLEKHLSFDMCRWTSALRDYQNGIEPDKIRQK
                     LGVSKIQWRELFIKLKQLNGEVK"
BASE COUNT          659 a          613 c          736 g          736 t
ORIGIN      
        1 agtggatgat attgagggaa tgatggcgca attgaaagca aaaaacattc ggctcatcaa
       61 tgaagaaccg cgcaccgcag ctgacgggaa gaagtatgct ttcatccacc ctgagtcaac
      121 gggtggcgtt ttagtggagt tgtatcaaat ctaaatgtcg tcaaacgttg aaacaatcat
      181 ggtagaggaa tgtggtctgg taaaagaccg tcctgtcatc gtcggtgttt ccggcggacc
      241 agacagtctc tgcctaatgg aaacgcttcg ccgcgcaggg taccatctca tcatcgggta
      301 ctttgaccac aaactccgcc ctgagtctgc actagatgcg cgcatggtag aaaaagctgc
      361 cgtgcggttg ggcttgctat gtattctggg gggcatggat gtgcgttcct atgcagaaga
      421 aaagaaactc tctattgaag aagccgcgcg gacgttaaga tatcgcttcc tctttgaact
      481 tgctagaaaa tataaggcac aagccgttgc cgttgggcac acggcggatg accaggtaga
      541 aacggttctt atgcactttt tgcgcggaag cggtatttcg ggcttgaagg gtatgtcgta
      601 ccggtcggtt atcaaatcct ttgatccaga tattccagtg gttcgcccgt tactggatat
      661 gtggcgtgaa gagaccgttc tgttctgtgc ggttaatggc ttacgcccaa gttacgactc
      721 gagcaatgag tctttgaact ttcaacgcaa ccgcattcgg catttgctca ttccctcact
      781 tgaaacatac aatccaaaat ttcgcgagac ggttttgcgc atgtcacatt ccctaaaagg
      841 ggattatgcc ttcgttatgg aaacgctgga acatgcctgg ggtaatgtag ttgtttctgc
      901 gaatgatgag attatcacct tcgatgtttt gcgcctatct tccatgccga tcggactcca
      961 acgtaacctg attcgccatg ccatgcagac tcttctccca ggcgtggaca ttgggtacca
     1021 aaccttgaaa cgcgcttctg attttattaa tgcttccagt gctacggttc cgcgtttgga
     1081 cttaaaagca ggtctacgta tgttccgcga agccgggctg attcatatct gcgcgcctta
     1141 tgctgagctt ccttttgacc gttggcccca aatggtggat gacaaatcgg ttccattaag
     1201 actgggcggg caggcttcac tagctggtgg gtggaaggtg atctgtgaaa gttggcgtct
     1261 gccaacattg gcgagagaac aagccgaaga aaacgaggac cagtttcagg tctggttgga
     1321 tgcggatgaa cttcctgaag aatttctatt gcgggctcgt cgtcagggcg atcatttttc
     1381 tccgctgggg atgggtggtc attctcaaaa actttcagac ttttttgtca acgagaagct
     1441 gccgcatcgt gcccgtgacc gttggcctct cttgtgtgtg ggtgatgaaa tcatttgggt
     1501 tccaggcttt cgcccggcac atcgctaccg tttgaaagag acgacccata atgtgattta
     1561 tttttcggtt caacatccgc ccgagaagat tgccccagat gaaaatcccc atccgtagtg
     1621 gatggggatt ttttatttta tttgacttcg ccgtttaatt gcttgagttt gatgaataac
     1681 tcgcgccact ggatttttga tacgcctagt ttttggcgga tcttatctgg ttcaatgcca
     1741 ttctgataat ctcgtaaggc agatgtccag cgacacatat caaaggagag atgtttttcc
     1801 aaaccggctt cttcgctaat atcttcaagc agatattcca aacggcgcgg tgaccagggg
     1861 aaagcttggt ctacagggct gtattgtgtg aggtattcgt tgtaggcttc aacccaatcc
     1921 tcggtcagtt caattttgcg ttctttgtag cggttggcag ggctggcata gcgcacaaaa
     1981 aggaagggac cgttcttacc agccaactca atgtgattga catgaatgcc aaggcattca
     2041 cttttcttga tggcagtttg aagcaagaga gacaacaagg tatatgggcg ggcatctggc
     2101 ttcttggcgc ggcgctgacg gtctgctgcc aaaagcaccg cttcgtattc ttcgtcggta
     2161 agaacctgtg gcaacgggct aatggcagat cgttgcagaa ctttttcggc agggtcaatg
     2221 cttaacgtgc cgtattgatg aagccagcgg aagaaagact taaccgatgt gatacgacgc
     2281 gacaaggttt tggggctgca tgccacgcca cgaccttttt ccatccactc gaagaagcgg
     2341 ttgagatcat cagtggtgac ggtgccaatg gcttgatctg gcggcaggaa ctgggtaagc
     2401 aaccgtacat ctgagagaaa ggctttgacg gtattggggg agcgtccctg atcgagcagg
     2461 aacatttccc aaccgttgat ggcggcaggt aacagcgttt tggcgctgat gtgggcagga
     2521 gcggagtttt ttgtcatacg aatcaacctt gcgtgtagta tttactcttg cgtatagttt
     2581 agcgaattta tagggtgttg tcaactggca aaaaaagaaa ccctcgaagt agaaaacttc
     2641 gagggttgag cgcgtcaaat actaaggcgg tggtaaataa tcccacgggt tgaccttgac
     2701 acctgccatg atctcaaagt gtaagtgtgc acccgaagaa cgac
//
LOCUS       DAOREF010000395         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25818_length_2739_cov_3.937407, whole genome shotgun sequence.
ACCESSION   DAOREF010000395 DAOREF010000000
VERSION     DAOREF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25818_length_2739_cov_3.937407"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..439
                     /locus_tag="PKI33_08910"
     CDS             77..439
                     /locus_tag="PKI33_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37170.1"
                     /translation="MKKYPIVLLSIVVLILSACGSKGDEVPDVLLTPPAIVVNASRDN
                     CPSIIAEVGMTIAWENIDTVPLPIKIEFLDENGKVTDTGLSVIDPETVFSTQFPNPGV
                     YRVYCTDNTDTYSTITVK"
     gene            complement(505..1077)
                     /locus_tag="PKI33_08915"
     CDS             complement(505..1077)
                     /locus_tag="PKI33_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNM37171.1"
                     /translation="MTTLAPTIYNRTFPRVSEVVRDITLILLGSWLIALFAQIEIPLP
                     FSPVPITGQTFAVLLVGALLGSKRGAAAMVAYIAQGAAGLPFFAGGASGFGILTGATA
                     GYLVGFIVAAYVVGLLAERGMERSFKTSIVPFLVGTIIIYAFGVTWLSTVVGGFGKAI
                     ELGLLPFVVGDILKLIAAAIALPAAWKFVR"
     gene            complement(1190..2437)
                     /locus_tag="PKI33_08920"
     CDS             complement(1190..2437)
                     /locus_tag="PKI33_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNM37172.1"
                     /translation="MILDETHPLEEVMVWGEPGIEALLGQLLPQSRSLFRSYYEVPKA
                     RGEFACMSSLIESEGIKVTRAKDAVADLLKTREIPELPGSIQDLKRILHQKADSTFEH
                     YRLAKQSDLIRDGITNISPLDVYNAVKRDIDVVLEEDIAQYGEQGALRLNYLLSLSKP
                     WPMANIFYGRDQSQALTDRIILSSLRWNIRKPEVRIFKDALHHMGYGAVLADIREGTI
                     EGGDVAMFKGYCFIGVGARTNINAVKEVYRQIGGSLKEHRIELIAVVNKQHEEDSNFF
                     ISPTSEHMQVMHLDMFWIPLTQDLVMAYTTELDHREVVRVWQEKGRILTERLGTFREF
                     MSIIGVEIFEVDEAEQKNFATNLLNFGNKKVIAALSSNPRVNAELERRGFTVKYAELT
                     KLVDGYGAVHCLTAPLKRRIPNS"
     gene            complement(2503..>2739)
                     /locus_tag="PKI33_08925"
     CDS             complement(2503..>2739)
                     /locus_tag="PKI33_08925"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNM37173.1"
                     /translation="MSQFPQKVQQAAEEYRPLVMAMYAYDLANAFHSFYHAVPVLQSE
                     DEKVKNARLRLVAAAKQAIANALHLLDIQSPDVM"
BASE COUNT          732 a          638 c          645 g          724 t
ORIGIN      
        1 gggaacaaaa tatttatggg ggtcgttgta caaaagatgc atcttttgta caacgacact
       61 cgttcaagga gaaattatga aaaaatatcc cattgttctc ttgtctattg ttgtcttaat
      121 cttatccgcc tgcggttcga aaggtgatga agtgccggat gtgctcctca cgccgcccgc
      181 cattgttgtg aacgcttcac gagataattg cccttccata attgcagagg tagggatgac
      241 cattgcttgg gaaaatatcg atacggttcc tctgccgata aagatcgaat tcttggatga
      301 gaatggaaaa gttaccgata cggggctctc tgtgattgac cctgaaactg ttttctcaac
      361 ccagttccct aaccctgggg tctatcgtgt gtattgtact gataacaccg atacgtattc
      421 aacaatcaca gtgaaataaa aacttgccat acgcatgcgt atggcaagta aagaacttaa
      481 ttttaggcgt gaagcacttt caatctatcg aacaaatttc caagctgcag gcaatgcaat
      541 ggctgcagcg attaacttga gaatatcgcc aacgacaaat ggcaacaatc ccaattctat
      601 ggctttgcca aaaccgccaa caacggtgct taaccaagtg acgccaaaag catagatgat
      661 gatggtgccg accaaaaatg gaacgatgga ggtcttgaag ctgcgttcca tgccgcgttc
      721 tgcaagcaga cctacaacat acgctgccac aataaagcca acgaggtaac cagcggttgc
      781 gccggtgaga atgccaaatc cgctggcgcc gcctgcgaag aaaggcaacc ctgccgcgcc
      841 ttgagcgata taagcgacca tggcagctgc accacgcttg gagccaagca aggcaccgac
      901 cagcaatacg gcaaacgtct gaccagtaat tgggacagga gagaagggga gtggaatttc
      961 gatttgagca aaaagcgcga tcaaccacga accgagtaag atgagggtga tatcgcgcac
     1021 gacctcagaa acacgaggaa aggtgcggtt gtagatggtt ggtgcaagtg tggtcatata
     1081 aaatctatct cctaagtaga atgtcaatag ttataacacg caaacccttg tgaagatttg
     1141 gatagggatt aatgtcattc tgtccccact gtaggatgac atgccatttt tatgaatttg
     1201 gtatacggcg ttttagcggt gcggtgagac agtggactgc gccgtatcca tcgaccagtt
     1261 tggtcaactc ggcgtatttg accgtgaagc cacgtctttc caactctgca ttcacgcgcg
     1321 ggttggaaga taatgccgcg atcacttttt tgttgccaaa gttgagtaaa ttcgtggcga
     1381 agttcttttg ttcggcttcg tccacttcga agatttctac accgattatg gacataaatt
     1441 cacggaaagt tcctaatcgt tccgttaaga tacgcccttt ttcctgccat acacgcacaa
     1501 cttcgcgatg atccagctca gtcgtgtatg ccatgaccaa atcctgtgta agtggaatcc
     1561 agaacatgtc caaatgcatg acttgcatgt gttcgctggt cgggctgatg aagaaattgc
     1621 tatcttcttc atgctgtttg ttgacgacag cgattagttc aatgcgatgc tctttcaacg
     1681 agccgccgat ctggcgatac acctctttga ccgcgtttat attcgttcgt gcgccgacac
     1741 cgataaagca atagcctttg aacatggcga cgtcgccgcc ttcaattgtc ccttcgcgaa
     1801 tgtctgctag aacagctcca tagcccatat gatgtagcgc atctttgaaa atgcgcacct
     1861 caggttttcg gatattccag cgtagagaag aaagaatgat acggtctgtg agggcttgcg
     1921 attggtcacg tccgtaaaag atatttgcca taggccaggg tttggagagg ctcagtaaat
     1981 aatttaggcg tagagcacct tgttccccat attgcgcaat gtcttcttcg agtacaacat
     2041 caatatcccg ttttactgcg ttgtatacat ctagagggga gatgttggtg attccgtctc
     2101 ggatcaagtc gctttgtttg gcgaggcgat agtgctcaaa ggtagaatcc gctttttgat
     2161 ggagaatccg tttcaggtct tgaatagagc cgggtaattc gggaatttca cgcgtcttca
     2221 ataaatctgc tactgcatct tttgctcgcg taaccttaat cccttcgctt tcgattaagg
     2281 aagacataca agcaaattca ccgcgtgctt tgggtacttc ataataactg cggaataggc
     2341 tgcgcgattg cggtaacagt tgaccaagca aagcctcaat gccgggctcg ccccaaacca
     2401 tgacttcttc taaaggatgt gtttcatcaa gaatcattta taccttcact tataaaaaca
     2461 gactaaatcc ttaaggattt agtctgttta ttgtactgat ttttacatca catctggcga
     2521 ctgaatgtct aatagatgaa gcgcattggc aatggcttgt ttcgccgctg ccaccaatcg
     2581 cagacgggca ttcttaacct tctcatcttc tgattgcaag accggcaccg catgatagaa
     2641 cgaatggaag gcgttcgcca gatcgtaggc atacatcgcc atcacaagcg gacgatactc
     2701 ctctgccgct tgctgcacct tctgcgggaa ctgcgacat
//
LOCUS       DAOREF010000396         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25889_length_2734_cov_3.870847, whole genome shotgun sequence.
ACCESSION   DAOREF010000396 DAOREF010000000
VERSION     DAOREF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25889_length_2734_cov_3.870847"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKI33_08930"
     CDS             complement(<1..1029)
                     /locus_tag="PKI33_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37174.1"
                     /translation="MTIESDSKPVRKTLGLTGITINAMALTAPGAFLWLLYQVQASAK
                     FDGVADIWPGVLLALLSAALTALSFGELARKYPEAGFRSAYHFADRFFARHKNSKHKS
                     VARLGKFAIGWAAHLYYWIYPGVLIAFMGILADYLLRYFGYQPTVFGDALLAASFAAF
                     AGFLALRGITGTTTTSVILNTIQLLTLLTFGILAITFRLINPAAFAPTEWAYPSALAV
                     LIPHSFDGLIFQMILAMILMVGFESSTALSVAATNAQRDIPRATILALIIQGLFSYLF
                     GYFIAGFALNAHIDATASQAPMGDIALQIGDLLLDGYGATLMVFFGFTVAIALLGGTL
                     TAINNGVRI"
     gene            complement(1019..1261)
                     /locus_tag="PKI33_08935"
     CDS             complement(1019..1261)
                     /locus_tag="PKI33_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37175.1"
                     /translation="MHETTSNISPPELIYLGMGLMIARILLDGTNTRGFTWLQWLQFL
                     VNGARIVMLWPLVLFIEKFEAWLKSGSSDDEVKHDH"
     gene            complement(1367..>2734)
                     /locus_tag="PKI33_08940"
     CDS             complement(1367..>2734)
                     /locus_tag="PKI33_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNM37176.1"
                     /translation="VFFMQAGFALVETGFTRNKNVVHTMMMNMMVFCIGAVGYWLTGF
                     AFQFGGVNAAYPEVSTVGAIAGEWAHSPITLGDWSGLLATPLLRFGQFGLLGGSGFAL
                     AGVEANAGILAFFLFQMVFMDTAATIPTGSMAERLKFSGFVVMGLWVSMFIYPLVAGW
                     IWGGGWLQNLGRIAGLGNGAVDFAGSGPVHMIGGCVGLAGAIVLGPRIGKFNKDGSAN
                     SIPGHNITIGVLGTIILFFGWFGFNPGSSLGFLGGFRNLAVIAAINTLLAGAAGGVSA
                     MTYMWLFGPTKKPDAGMSVNGILAGLVAITAPCAFVAPWAAVVIGTIAGVLVIWASTA
                     LEKLKIDDPVGAVPVHMVNGAWGVLAVGIFANGNPDTAAWNGMESAVTGLIYGGTTQI
                     VAQLAEVVSIFVVVVGLSWVFFKAMYALKLMRVSREDELAGLDLPEMGSLAYPVDWEA
                     PADAV"
BASE COUNT          807 a          676 c          707 g          544 t
ORIGIN      
        1 gattcgtacg ccgttgttga tggcggttaa tgtcccaccg agcagggcga tggcgacggt
       61 aaagccgaaa aataccatta aggtggcacc ataaccgtct aataacaagt cgccgatttg
      121 tagggcaata tcgcccatgg gtgcttgtga tgcagtggca tcaatatggg cgtttagtgc
      181 gaaccctgca atgaaataac caaaaagata tgagaataag ccttgaatga ttaacgccag
      241 aattgtcgcg cgcggaatgt cgcgttgggc attggtagcg gcaacgctca atgcggtaga
      301 cgattcaaac ccaaccatta ggatcatcgc tagaatcatt tggaagatca gcccgtcaaa
      361 gctgtggggt atgagaacgg ctaaggcgga gggataggcc cattctgtgg gggcaaatgc
      421 ggcggggttg atgaggcgga atgtaatcgc aagaatgccg aatgtgagta gagtcaataa
      481 ctgaatggtg ttcaagatca cgctggtggt ggtggtgcct gtaattccgc gtaatgcgag
      541 gaaaccagca aaggcagcga acgaagcggc taagagtgca tctccgaata cagtaggttg
      601 gtacccaaag taacgtagta aataatcggc aagaatgccc atgaaggcaa tcagaacgcc
      661 ggggtagatc caataataaa gatgtgcagc ccaaccaatt gcgaattttc ccaaacgtgc
      721 tacagactta tgtttggaat ttttatgacg cgcaaaaaaa cggtctgcaa aatggtaagc
      781 gcttcgaaac cccgcttcag gatattttct agcaagctct ccaaatgaaa gggctgtgag
      841 tgctgctgaa agtaatgcca ataaaacccc gggccaaata tctgcaacac catcaaactt
      901 ggcagaggct tgtacttggt agagcagcca taagaaagcg ccgggcgcag tcaacgccat
      961 ggcattgatc gtaatccctg ttaacccaag tgttttacga actggcttgg aatcggactc
     1021 aatggtcatg ctttacctcg tcgtctgatg aacctgattt caaccaggct tcgaattttt
     1081 caatgaacaa taccaggggc cagagcatga cgatacgcgc tccgttgacc agaaactgaa
     1141 gccattgcag ccaggtgaat ccgcgtgtgt tggtgccgtc taacaagatg cgcgcgatca
     1201 taagccccat gccaaggtat attaattcag gtggagagat attggatgtt gtctcatgca
     1261 tgacaatgct gtactctatc tggaaattgg tatagagact ccggaaggcg gcgatgtaat
     1321 tacccttccg aagcctcgat gttgaaatta cgggtgagta tagagactat actgcgtcag
     1381 caggagcttc ccagtctaca gggtaggcga gagagcccat ttcaggaagg tcgagacctg
     1441 ccaattcgtc ttcgcgagaa acacgcatca acttgagggc atacatggct ttgaagaata
     1501 cccatgagag acctacaacg accacaaaga tactgaccac ttcagccaac tgggctacga
     1561 tctgagttgt accaccatag attagaccgg ttacggcgct ttccatgccg ttccatgcag
     1621 cggtatcggg gttgccattg gcgaaaatgc cgacggcgag gacgccccaa gcgccattga
     1681 ccatgtggac aggaactgca ccgacgggat catcaatctt taatttctca agagcggtgg
     1741 atgcccaaat taccaacaca cctgcaatag taccgatcac aacagctgcc cacggagcaa
     1801 caaatgcaca aggagctgtg atggcaacca agccagccaa aataccattg acggacatgc
     1861 cggcatctgg tttcttggtg ggaccaaaca gccacatata agtcattgca gaaacgccac
     1921 cagcggcacc agccaacagc gtgttgatgg cggcgataac ggctaagttg cggaagccgc
     1981 ccaaaaatcc aagggaagag ccggggttga aaccaaacca accgaagaac aagataatgg
     2041 ttccaagcac gccaatggtg atgttatgtc ccgggatcga attggcggag ccgtctttgt
     2101 tgaacttacc aatacgagga cctagcacaa ttgcgccagc taaaccgaca caaccaccga
     2161 tcatgtggac aggacccgaa ccggcaaagt ccacggcgcc attgcccaaa ccagcgatac
     2221 gacctaagtt ctggagccaa ccaccacccc agatccaacc agcaaccaaa ggataaatga
     2281 acatacttac ccagagaccc attaccacaa aaccagagaa cttgaggcgt tcagccattg
     2341 agccggtggg gatggtagcg gctgtatcca tgaagaccat ctggaataag aagaaggcga
     2401 gaataccagc gttcgcttct acaccagcca atgcaaaacc acttccaccg agaagaccaa
     2461 actgaccaaa gcgcaacaag ggggttgcta aaagcccgct ccaatcgcca agggtgatcg
     2521 gggagtgtgc ccattcacca gcgatagcgc caacagtgga tacttctggg taggcagcat
     2581 tcacaccgcc gaactggaag gcaaagccgg ttaaccagta gccaacagca ccaatacaga
     2641 agaccatcat gttcatcatc atggtgtgga cgacgttctt gttacgggtg aagccggttt
     2701 ccaccagagc gaacccagcc tgcatgaaga agac
//
LOCUS       DAOREF010000397         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_25930_length_2732_cov_4.287635, whole genome shotgun sequence.
ACCESSION   DAOREF010000397 DAOREF010000000
VERSION     DAOREF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25930_length_2732_cov_4.287635"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PKI33_08945"
     CDS             complement(<1..305)
                     /locus_tag="PKI33_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNM37177.1"
                     /translation="MKSAVVEIPIEKLMEQLPESYTVADRELVMRAYTFAEEAHREQK
                     RNSGEPYINHCIAVASILTELKVPADVIAAGLLHDTVEDTPVTLADIRREFGDVVRLL
                     "
     gene            complement(429..881)
                     /locus_tag="PKI33_08950"
     CDS             complement(429..881)
                     /locus_tag="PKI33_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNM37178.1"
                     /translation="MISTEILSQFSLFDGLPESLLKEIAGISNEVSVKKDDFVFREGE
                     KADKLYFLLKGSIVLRVKLTSRPESITVSFVSSPYRSFGWSGVVAPHHYTSSAEADED
                     SSLLIIPAEPFMTLLEKNPEHGFTVMKRIAEVIADRLRNSHQALLKTI"
     gene            995..1471
                     /locus_tag="PKI33_08955"
     CDS             995..1471
                     /locus_tag="PKI33_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNM37179.1"
                     /translation="MSVTNNVTRFLDSRKIKYIAYELPAEKLGAVEAAQLLNVPQEQV
                     FKSIVTKREKGKPVLTVIPGPRVVDLKLLASFLGEKKMHLPTEREAEQLTGLQAGGIS
                     PLALINKGFQVVIDASAQNFETIYISGGQRGLNIQLGAKDLAGLVNAKFGDVSREG"
     gene            complement(1521..1982)
                     /locus_tag="PKI33_08960"
     CDS             complement(1521..1982)
                     /locus_tag="PKI33_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37180.1"
                     /translation="MRRLVITLFLFLFLVSACGAPSPDDPVSNDTVTVHTPAAQETSN
                     YIPSPADGSLTRGEVSLEAKELASLESYPVQFMLQLKGELPTPCHQLRIATNPPDKDH
                     KVLVDVYSVVNPAEICIQVLVPFEIAFPLGSYPEGKYSLWVNGEMVAEFQA"
     gene            complement(2029..2484)
                     /locus_tag="PKI33_08965"
     CDS             complement(2029..2484)
                     /locus_tag="PKI33_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37181.1"
                     /translation="MRYLNFFLIAVLLSACSTVPTAEPTSTAVPPYRITPDENPYEPK
                     LEDLGRQIDEVTITSVNLAELYDYSPPRTAIGLIGYMPSVCDELRVEISPPDENFNVF
                     IQVYSLVNPDVQCDNVFQQFEARILLGTYSPGRYYVWVNDAPVGDFVSY"
BASE COUNT          722 a          612 c          676 g          722 t
ORIGIN      
        1 agcaagcgca ccacatcccc aaactcgcgg cgaatatctg cgagggttac aggcgtatct
       61 tctacggtat catgcaacaa acccgccgca ataacatcag cgggtacttt taactcggtc
      121 aaaatacttg ccacggcaat gcagtgattg atatatggct cccctgaatt gcgcttctgt
      181 tcacgatgcg cttcttctgc aaacgtgtag gctcgcatca cgagttcccg gtctgcaaca
      241 gtataagact cggggagttg ttccattaat ttttcgatag ggatttctac gactgccgat
      301 ttcatggatg aattttacta cgcccgcctc tctctcggac tttgatatgc ctctgtttcc
      361 tgatggcttt ccctccgctg tggcttgata taaaaaccac tggttttttc aaaccagcgg
      421 ttcgtttctt agatggtctt caacaatgcc tgatggctat ttcgcaaacg gtctgcgatc
      481 acttcggcaa tacgcttcat aactgtaaag ccatgctcag ggttcttttc cagcaaggtc
      541 atgaagggtt ctgcagggat aataagcagg cttgagtctt cgtctgcttc ggcgctggag
      601 gtgtaatgat gcggcgcaac cacaccagac cacccgaaac ttcggtacgg tgaagagaca
      661 aaagacacgg tgatgctttc tgggcgagag gtcaatttga cgcgcaagac gatgctgccc
      721 ttgagtagga aataaagctt atcggctttt tctccttcac gaaatacaaa atcatctttc
      781 tttactgaaa cttcattgct gatgcccgcg atctctttga gcagagattc tgggagcccg
      841 tcgaacaggc tgaactggga aagaatttcc gtgctgatca tgcaatagcc tccaatcttc
      901 agggtgaaat agggaaaatg gggtcgatca gtttcgagtt tagaacgaag cgcagcacct
      961 ttatgtttat aatattctac aggaaattgt caatatgtca gtcacaaata atgtcacaag
     1021 atttttggat tccaggaaaa ttaaatatat tgcttatgag cttccggctg aaaaattagg
     1081 cgcagtggaa gcggctcaac ttttgaatgt gccgcaggaa caagttttca agtccatcgt
     1141 tacaaaacgc gagaaaggta agccggtatt gacggtgatc cccggtccgc gggtggtgga
     1201 tttgaaattg ttggcgagtt ttctcggcga aaagaaaatg catttgccta ccgaacgtga
     1261 agccgagcag ttgaccgggc tgcaagcggg cggaatctcg ccgctggctt tgatcaacaa
     1321 agggtttcag gtggtgatcg atgcctctgc tcaaaatttt gagaccatct atatttcagg
     1381 cggtcagcgt ggactcaata ttcagttggg cgcaaaggat ttggcagggc tggtcaacgc
     1441 aaaatttggc gatgtaagcc gagagggata aataaaaatc aaaagaggaa agaagctgaa
     1501 gcgcttcttt cctctttttg ttacgcttga aattcagcta ccatttctcc attgacccat
     1561 aaagaatatt tcccttcagg gtaactccct aatggaaaag cgatctcaaa tgggacgaga
     1621 acttgaatgc agatctccgc cggatttaca acagaataca catctaccaa aactttgtgg
     1681 tctttatcgg gtgggttggt tgcaatgcgt aattgatggc agggtgtggg cagttcacct
     1741 tttaattgca acatgaattg aacggggtaa ctttccaacg aggcaagctc ttttgcttcg
     1801 agagagactt ctccacgtgt caaagagccg tctgccgggc ttgggatgta gttgctggtt
     1861 tcctgtgccg ccggcgtatg gacggtgact gtatcgttgc taacggggtc atctggcgat
     1921 ggggctccac atgcagatac aagaaatagg aatagaaaca atgtaataac caaacgtctc
     1981 atttttaatt ctcctgcctg attaacgaaa aaatgtatcg aatggttcct aataggaaac
     2041 aaaatcaccc acaggggcat catttaccca aacgtagtac ctgccggggg aatatgtgcc
     2101 aagcagaatc cgcgcctcaa attgttgaaa cacattgtcg cactgcacat ctgggttgac
     2161 caagctatat acctggataa agacattgaa gttttcatcc ggcgggctga tctccactcg
     2221 cagttcatcg caaacgctgg gcatgtaacc gatcagccca atggcggtcc gcggcgggct
     2281 atagtcatag agctctgcaa gatttacaga agtaatggtg acttcatcaa tctgcctgcc
     2341 aaggtcttct aatttgggct cataaggatt ttcatcgggt gtgatgcgat aagggggaac
     2401 tgccgttgag gtcggctcag cagtagggac ggtgctacaa gcactgagca agaccgcaat
     2461 caggaagaaa ttaagataac gcataccgct gtgattctcc gacgcagata atatcacggt
     2521 cttgcgatac aaaaaactca ctcaggtagg gtctgagtga gttttttgta gggttatttt
     2581 tttagcgctt ccaaggcttg caaaagaatt ccgcccgtat ttacgatagg acccgcattc
     2641 gggtctttcc acatgaaatc tccggtgcca agtccgtggt aaattccgct aggagtggca
     2701 atacagatca agacatggtc cgcttgtttg gg
//
LOCUS       DAOREF010000398         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26009_length_2728_cov_3.857838, whole genome shotgun sequence.
ACCESSION   DAOREF010000398 DAOREF010000000
VERSION     DAOREF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26009_length_2728_cov_3.857838"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PKI33_08970"
     CDS             complement(<1..1052)
                     /locus_tag="PKI33_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNM37182.1"
                     /translation="MQTTLDPERQKQAKEYARIRRRLWLVDTLLNAAYAIAWLVFGWS
                     ISLREWLATITTNEWLLVALYVAIFGGIFSVFTLPLGYYSGFILPHRFDQSNQSLKDW
                     VIDRIKGLAIGAPLGLLMLELLYLALRLTGSLWWLYAAGGLLLFNVILSNLAPVLIMP
                     LFNKYVPLGDEHKDLEERLLNLAKRANTKVKGVFKFDMSKRTKAANAALTGMGNTRRI
                     ILGDTLINEFTPDEIETVLAHELGHHVHKDIPFLIISGTLLTTMGLFIASQALNYSVT
                     FFSFNSVADVAAFPALSLILGAYGLLTQPLENYLSRWRENMADDYALVSTGKHEAFAS
                     AFTRLANQNLGEVDPE"
     gene            1111..1719
                     /locus_tag="PKI33_08975"
     CDS             1111..1719
                     /locus_tag="PKI33_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HNM37183.1"
                     /translation="MNFISTLGFAGTIIDLLRAAPQLVRLLRERKSFGVSVDTSGTSC
                     VVSLGWTTYGIITNQIFVALASGAIASFFFIITVAALRLGRSAREFRVTPLWFIVLFA
                     AVTFFGKTGLGLMLSISALISNVPQIRVAYKEENLSGLSLGTWLLTLSGGLIWLIYGI
                     LQKDLTIMVSTIFQSVTSAIIIGLKLFKQRTENTTATRFISD"
     gene            1750..2568
                     /locus_tag="PKI33_08980"
     CDS             1750..2568
                     /locus_tag="PKI33_08980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM37184.1"
                     /translation="MTAFPPNAYELIRPLLLNMDIHLIGRSILAKQTPAQIFVDNHEH
                     PKALFAQAGHRYILAGDSERDSFNLGIQKLFTNVIFPREIAAGHEGFALYYDSSTWEK
                     RIDTLLSGKKTIHADREYYVCKKLKHKWQDFLPEGFEIKTVDAALLADSNLKHLETLK
                     EEMTSERPSVEDFLAKSFGVCAIYQGELAGWCLSEYNANGRCEIGIETISDYRKRGLA
                     TALTLAFLEHAFSHGITEIGWHCFKRNEASAKTALKAGFEKVCDYQSFIVLLKE"
BASE COUNT          780 a          665 c          612 g          671 t
ORIGIN      
        1 ttctcagggt cgacctcgcc gaggttttgg tttgccaaac gagtgaaagc agaagcaaag
       61 gcttcatgtt tgcccgtgga cactaaggca taatcatctg ccatgttttc acgccagcga
      121 gaaagataat tttcgagagg ttgagtcaac aagccatatg cgccaagaat tagagagagt
      181 gcagggaatg cagccacatc tgcgacagaa ttaaaggaga aaaaggtcac agaatagttc
      241 aaggcttgag aggcaatgaa cagacccatg gtcgtaagaa gcgtaccgga gataatcaag
      301 aagggaatat ccttatggac gtgatgtccc aactcgtgtg caagaaccgt ctcgatctca
      361 tccggtgtaa attcgttgat gagggtatcg ccaagaatga tacgacgcgt attgcccata
      421 ccggtcaatg cggcattggc agctttggta cgtttggaca tatcgaactt gaagacacct
      481 tttactttgg tattggcgcg ttttgcaaga ttgagcagac gttcttcaag gtctttatgt
      541 tcatcaccaa gtggaacata tttattgaac aacggcataa tgagcaccgg cgcaaggttg
      601 gaaagaatga cattaaagag tagcaatcca ccagcagcat ataaccacca gagactccca
      661 gtgaggcgca atgcaagata gagcaattca agcatcaaca aaccaagcgg cgcaccaatg
      721 gcaagtccct tgatgcggtc gataacccaa tctttgagcg attgattgga ttggtcaaaa
      781 cgatgtggga gaataaagcc actgtaataa ccaagcggca gagtaaagac agagaatatg
      841 ccgccaaaga tggcaacata taatgcgact aacaaccatt cattggttgt gatcgtggcg
      901 agccactcgc gcagagaaat gctccaaccg aatacaagcc aggcaatggc ataggcggca
      961 ttcaatagtg tatcaaccag ccaaaggcgg cggcgaatac gtgcatactc ttttgcttgt
     1021 ttttgtcgtt cggggtcaag ggtcgtttgc ataggcggaa ttataatatg cgctcactat
     1081 caccagtaaa acttccctat taggacaaaa atgaatttca tctcaactct tggatttgca
     1141 ggcaccatca tcgaccttct tcgcgccgcg ccacaactgg tacggctttt gcgtgaacgg
     1201 aaatctttcg gcgtctcagt ggacacttct ggcacgagct gtgtcgtcag cctcgggtgg
     1261 actacttacg gcatcatcac aaatcaaatt ttcgtcgcac ttgcatctgg cgccatagcc
     1321 agcttctttt ttattatcac agtggctgcc ttgcgccttg ggcgaagcgc gcgtgaattt
     1381 cgcgtgaccc cgctttggtt tattgtcctc ttcgcggcag taactttttt tggaaaaacc
     1441 ggtctggggc taatgctttc gatcagcgca ttgatttcga acgtcccaca aatacgagta
     1501 gcgtacaaag aagaaaacct ttctgggctt tcgctgggca catggttgct gactctctca
     1561 ggtgggttaa tctggctgat ctatggaatt ctgcaaaaag acctcacgat tatggtttcc
     1621 actatctttc agtcggttac cagcgcgatc attattggtt tgaagctctt caaacaacga
     1681 acagaaaata ccaccgccac acggttcatt tcagattaac taaaaagata aaagattgga
     1741 aactcaactt tgaccgcatt ccctcccaac gcctacgaac tcatccgccc gctgctgctc
     1801 aacatggata ttcacctcat cggtcgttca attctcgcca aacaaacacc agcccagatc
     1861 tttgtggaca atcacgaaca ccccaaagcc ttgtttgccc aagcaggtca cagatatatt
     1921 ctcgccggtg actccgaaag agatagcttc aatctcggaa ttcaaaaatt atttacaaac
     1981 gtcatttttc cacgcgagat cgcagcaggg catgaaggct ttgcgcttta ctatgattca
     2041 tctacatggg agaaaagaat tgatactctg ctttcaggaa aaaagacaat ccatgcagat
     2101 cgcgaatact atgtctgcaa aaagttaaag cacaaatggc aagattttct accagaagga
     2161 tttgaaataa aaactgtaga cgcagcactt cttgcagatt ccaacctaaa acacctcgag
     2221 acgctcaaag aagagatgac ctcagaacgc cccagcgtgg aagatttcct cgctaaaagt
     2281 tttggggtat gtgccatcta ccaaggcgaa ttggcaggtt ggtgtctctc tgaatacaat
     2341 gccaatggtc gctgcgagat cggcatcgaa accatcagtg attatcgcaa gcgcgggctc
     2401 gctacggcac tcactctcgc ttttctcgaa catgccttct ctcatggcat aaccgaaatc
     2461 ggctggcatt gtttcaagcg caacgaagcc tctgcgaaaa cagcgctcaa agccggcttc
     2521 gaaaaagtct gtgattacca gtcgtttatc gtcttactca aagaatagga taaaagaagc
     2581 tgtcacaaaa aacgtgacag cttcttcgct tctttcttag ccgataattg actctactta
     2641 cctagcattg gctttctctg cagccaatct cgcgatcacc tgctccgaca tcatcacctg
     2701 cccatacgac ttgaacgccg ccaaaaac
//
LOCUS       DAOREF010000399         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26026_length_2726_cov_4.009734, whole genome shotgun sequence.
ACCESSION   DAOREF010000399 DAOREF010000000
VERSION     DAOREF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26026_length_2726_cov_4.009734"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKI33_08985"
     CDS             <1..835
                     /locus_tag="PKI33_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37185.1"
                     /translation="KGWHQTVKFKRFFPFIVLLLLSCVLLVILPAVNFFNSEFIQDPA
                     ERLQFSLTQGLKFSALILLFAWIGVGLSSLLTLIKDGRSRKLISWVVYLGFLFFCIIT
                     PILGVVFVYGVSYAGSSWQKFPTPPETPKAIAAAGYSTVVIETEEGHYFYCDTSQLEL
                     CWQPENKPSSLIIPVSYGSVEQIEGKPIVPAPGRVVSLVGVDHQDGPNHTEIYYAILK
                     NGSIWYLQLDQSSAGLLAGFSSLIAIPIVFVSSILFIGMVLAAFLHWLANRIWREKEA
                     A"
     gene            complement(873..1286)
                     /locus_tag="PKI33_08990"
     CDS             complement(873..1286)
                     /locus_tag="PKI33_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37186.1"
                     /translation="MHRHKRYRYGPPLFPRPFRGWIWMLLIAFMMFGGRGNWWPGILI
                     LVGVMFLFSSLFRDQRPPEPPPPPMQHPPFDMPPPPPARRADTPPNEPVRRADLLPAT
                     CPNCGGPVRSNDLKWTSSHSVACSYCGSNIETRKE"
     gene            complement(1337..1741)
                     /locus_tag="PKI33_08995"
     CDS             complement(1337..1741)
                     /locus_tag="PKI33_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNM37187.1"
                     /translation="MASKTVYLNWDSESKFVLNDRDNYQLRVNGAGNIGSADLLPMSL
                     IGCSAYDVVEILEKQRQELHQLKVTAEAQQDDTPPWKFRKIHIRYQVIGKGIDLEKAK
                     KAIQLSEEKYCSVYATLRDVIEITHEVEVLEA"
     gene            1795..2463
                     /locus_tag="PKI33_09000"
     CDS             1795..2463
                     /locus_tag="PKI33_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNM37188.1"
                     /translation="MIIFLVIAFVFAGLESLALYRNWVKVEWVAKPAVMVCLLVWLFT
                     SVGLEGALLWFGLGLLFSLVGDVLLLISLDKLFLAGLVAFLFAHVFYVVGFNIPLPAI
                     SAWSVILAVLIGWGGAKVIRRILVSVLEKEQGRMRLPIIIYSVVISMMLLSAMVKLND
                     LSWHAGASILVALGAFLFYLSDIVLAWNKFVSPFNYGRIYNIALYHLGQIMLIAGVIV
                     QYHR"
     gene            2509..>2726
                     /locus_tag="PKI33_09005"
     CDS             2509..>2726
                     /locus_tag="PKI33_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HNM37189.1"
                     /translation="MSRPIPADEVRIFSGTSNLPLAKSIAAELDIPLENTIVKKFSND
                     NLYLQLGASVRYRRVYIVQSLTPPVNDN"
BASE COUNT          590 a          579 c          718 g          839 t
ORIGIN      
        1 aaaaggctgg catcaaaccg tgaaattcaa acgcttcttt ccctttattg ttttgctcct
       61 tctttcgtgt gtattgcttg ttattttgcc tgctgtaaat ttctttaatt ccgaatttat
      121 ccaagatcct gctgagcggc ttcaattttc gttgacccag gggcttaaat tttctgcgct
      181 cattcttttg tttgcatgga tcggcgttgg gcttagtagt ttattgacat tgatcaaaga
      241 tgggcgatct cgtaagctca tttcgtgggt ggtgtatctt ggttttcttt tcttctgtat
      301 catcacgccg attcttggcg ttgtatttgt gtacggtgtt tcatatgctg gttctagttg
      361 gcaaaaattc cccaccccgc ctgaaacacc aaaggcaatc gctgcagccg ggtatagtac
      421 ggtagtgatt gaaacagaag agggacatta tttctattgc gatacgtctc aacttgagtt
      481 gtgttggcag ccagagaata aaccgagtag cctaatcatc cctgtgtctt acggctcagt
      541 agaacaaatt gaaggcaaac caattgtccc tgcccctggg agagttgtga gtttggtagg
      601 cgtagatcat caagatggtc caaaccatac tgaaatctat tatgcgattc taaagaatgg
      661 cagtatttgg tatttgcaat tggatcagag ttcagcaggt ttactggcag gtttttcttc
      721 tcttattgca attcctattg tctttgtgag tagcatactc tttataggga tggtgcttgc
      781 tgcattcttg cattggttgg caaaccgcat ttggcgtgag aaagaagccg cataaataaa
      841 aaaagctctg ggtccagaac ccagagcttt ttttattctt ttctcgtttc aatgttcgac
      901 ccgcaatacg agcaggcgac ggagtgagaa ctggtccact tcaggtcgtt tgagcggacg
      961 ggaccaccac agttggggca ggtggcgggc aacaggtctg cgcggcgcac tggctcattt
     1021 gggggcgtgt ccgctcgccg ggcgggggga ggcggaggca tgtcaaatgg cgggtgttgc
     1081 atgggcggtg gtggaggctc gggcgggcgt tggtctcgaa agagagagct aaagagaaac
     1141 atgacgccca ccaagatgag aatgccgggc caccaattgc ccctgccgcc gaacatcatg
     1201 aaagcgatta agagcatcca gatccagccg cgaaagggtc tagggaagag gggcggtcca
     1261 taacggtaac gtttatgtct gtgcatgaaa tatcctttgg gtacgcatac tatacgcgcc
     1321 actctcattg aagttgctat gcttccaaca cttctacctc gtgcgtgatc tcgatcacat
     1381 cgcgcagggt ggcgtacacc gagcaatatt tttcttcgct gagttgaatg gctttctttg
     1441 ccttttcaag gtcgatgcct ttgccaatga cctgataccg aatgtggatc tttcgaaact
     1501 tccagggtgg tgtgtcatct tgctgcgcct ctgccgtgac cttgagctgg tgcagttcct
     1561 gacgctgttt ctccagaatt tcgaccacgt catatgccga acagccaatc agcgacatgg
     1621 gtaacaaatc ggcggaacca atatttcccg cgccattgac gcgtaattga taattgtctc
     1681 ggtcgttgag tacaaatttg gactctgagt cccagttgag atatacagtt ttcgatgcca
     1741 tatccgcagt atagcatggg atgccttcgt ggtaggatag agcaggaggg cactgtgatt
     1801 atctttttag tgattgcgtt tgtatttgca gggttggaat ctctggcatt gtatagaaac
     1861 tgggtgaaag tggaatgggt tgccaaaccg gcggtaatgg tttgcttact cgtctggtta
     1921 ttcacctctg ttggattaga aggggcgttg ttatggtttg gtttggggtt gctcttttct
     1981 ttggtgggtg atgtgttatt gctgatctcg ctcgataaat tgtttttagc gggactggtg
     2041 gcattcttat ttgcgcatgt gttttatgtg gtcgggttta atatcccttt gcctgccatc
     2101 tcagcgtgga gtgtgattct tgcagtgttg atcggttggg gcggggcaaa ggtcattcgc
     2161 cgcattctgg tttctgtcct cgaaaaggag cagggacgta tgcgcttgcc gatcatcatc
     2221 tacagtgtcg tgatctcgat gatgctgctt tctgccatgg tgaaactcaa cgatttatct
     2281 tggcatgcgg gggcatctat tcttgtggcg cttggtgcgt ttttgtttta tctttccgat
     2341 atcgttctgg catggaataa attcgtctcg ccatttaatt atgggcgtat ctataacatc
     2401 gccttgtatc atcttggtca gatcatgttg atcgccgggg tgatcgttca gtatcaccgc
     2461 tgatttgtgt cataatcgga atacaattca aggtatatgg agaatcatat gtcgcgtccc
     2521 atccccgctg atgaagttcg cattttcagt ggcacgtcta atttgccgct tgccaaaagc
     2581 attgccgcag agttggatat tccattagaa aatacaatcg tgaagaagtt cagtaacgat
     2641 aatttgtatt tgcagcttgg cgccagtgtc cgctatcgcc gggtatacat tgttcagtcg
     2701 ctcacgccgc cggtcaacga taactt
//
LOCUS       DAOREF010000400         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26110_length_2721_cov_4.739685, whole genome shotgun sequence.
ACCESSION   DAOREF010000400 DAOREF010000000
VERSION     DAOREF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26110_length_2721_cov_4.739685"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKI33_09010"
     CDS             <1..861
                     /locus_tag="PKI33_09010"
                     /inference="COORDINATES: protein motif:HMM:NF020671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HNM37190.1"
                     /translation="PGETLGGGAVVDHQPKGRHKRFDADVLKSLESLAQGSPADVLFE
                     AALALQIASIKDMVSKSRLEVKVAESALNELLQNGQLISLENSPKPEALAVAFPYWSK
                     LSTTILDTVNGYHSQYPLRRGIPREELKSKLKLTPKVFNAAIAFLASKSDLVDERGAV
                     LKPGHEIKFDGQNQVKVQALRRKFESNPYATPSVKECQAEADEEIVNALIETGELVLV
                     SQDVIFRKVDYDEMVAKIKNTIQTQGQITLAEVRDMLNTTRKYVQALLEHLDATGVTM
                     RDGDARKLRK"
     gene            869..>2721
                     /locus_tag="PKI33_09015"
     CDS             869..>2721
                     /locus_tag="PKI33_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM37191.1"
                     /translation="MKLIRDFLKPPVFDDEQKTHQAYLLNIIIWGLIAIPIPYVIYAQ
                     IFLSGTSTRALTQSIIGEAINIFLLILLKRGHVRTVSLLQVIAFWIFFTVSAATGIGV
                     QGESYMLGYPLVIMISGILLGNRFTLGLTTLSLVSGLLMVVAESNGLITKDVMRPPFL
                     TWILSFAIFPMSAVLQYLSSRTVNNALKRAKESEEKYRLISSVSSDYAFESRINDNEL
                     GEAIWIAGAFEKMTGYTPDEYINAGGWYAHIHPDDLDQDMEDMKKLLRNEAVLGSEIR
                     TFSKNGAIRWERVFAHPIWDEKENKLVGILGAVQDITERKVADQKLRDSLRQQEAILN
                     NIPDMAWMKDLDSRYIAVNEQFLRISGLRAEDVVGKTDKDIWEASFAEMYRHDDLEVM
                     QSGVRKTVEELQKDNTGREYWVETTKTPIRNESGQVIGTTGIARDVSERKKNEEAEHR
                     RREMLEKVIELGKQVTEAGDLDTILDKIWHGVHDHLGFDRLGIFLYNPELASMDSTIG
                     TDRDGNKVATYGNSFSLTKWSTFQLLLEKPDGLYSTKNYDVENDIPPENEMYGVKHYA
                     AVAVWAGNKPVAAITVDQLITHRPITPEQLEALRLFAGYAGLGIENARLNMA"
BASE COUNT          809 a          559 c          670 g          683 t
ORIGIN      
        1 cccggtgaaa cacttggcgg tggcgccgta gttgaccatc aacccaaagg tagacacaaa
       61 cgatttgatg cagatgtttt gaaatcactc gaatcattgg cacaaggttc tcccgctgat
      121 gtcctttttg aggcggcatt ggctttgcaa attgcgtcca ttaaagacat ggttagcaaa
      181 tctaggctag aagttaaagt tgcagagtcc gctttgaatg aactattaca gaatggtcag
      241 ttgatttcac tggaaaattc tccgaaaccc gaagcgcttg ccgtggcatt cccctattgg
      301 agcaagctaa gcacaacgat actggataca gtcaacggat atcacagcca atacccacta
      361 cggcgtggga ttccgcgtga ggaattaaag agcaagctaa agttaacacc caaggtcttt
      421 aacgcagcaa ttgcattcct cgcttcaaaa agcgatttgg tagatgaacg cggagcggta
      481 ttgaagccgg ggcatgaaat aaaattcgat ggacagaatc aggtcaaggt ccaggcattg
      541 aggcggaagt ttgaatccaa cccttatgct accccaagcg tcaaagaatg tcaggcagaa
      601 gccgatgaag aaatcgtcaa tgcgttgatt gaaacaggtg agttggtgct cgtttctcaa
      661 gatgtcatct tccgtaaagt agactatgat gaaatggtgg cgaaaatcaa aaatacgatt
      721 caaactcaag gacagatcac gttagctgag gttcgcgata tgttgaacac gactagaaag
      781 tatgtccaag ccttgttaga gcatttggat gccactggtg tgaccatgcg agatggagat
      841 gcaagaaagc taagaaagtg aaggcgggat gaaactcatt cgagactttc tcaaaccacc
      901 tgtctttgac gatgagcaaa aaacacatca ggcgtatttg ctcaatatta ttatttgggg
      961 gctgatcgca atcccgatcc cttatgtgat ctatgctcag atatttttat ctggcacttc
     1021 aacaagggct ttaacgcaaa gcatcatcgg cgaagccatt aacatatttc ttcttattct
     1081 cttgaagcga ggacatgttc gtaccgtttc gcttttacaa gtcatagcat tttggatatt
     1141 ctttactgtt tcagcagcaa ctggtattgg ggttcaaggc gagtcgtata tgctggggta
     1201 tccgcttgtc atcatgatat cggggatttt gctcggtaat cggtttacgt tgggattaac
     1261 cacattgtct ttggtttcag ggcttttgat ggtggttgca gaaagcaatg gactgatcac
     1321 caaagatgtc atgcgcccgc cgttcctaac atggatcctc agctttgcca tcttcccgat
     1381 gagcgcagtt ttgcaatacc tatcatcgcg gacggtaaac aatgctttga aacgcgcaaa
     1441 ggaaagcgaa gaaaaatatc gtttaatctc aagtgtcagt tcagactatg cgtttgaaag
     1501 ccgcatcaac gacaatgagt tgggagaagc gatttggatt gccggggcct ttgaaaaaat
     1561 gaccggctat actcctgatg aatatatcaa tgcggggggt tggtatgcgc acatccaccc
     1621 agatgatctc gaccaagaca tggaggacat gaaaaagctt ctacgaaatg aagcggtgct
     1681 tgggtctgag attcgtactt tttcaaaaaa tggagcgatc cgttgggaac gggtctttgc
     1741 gcacccgatc tgggatgaaa aagaaaataa gttagtgggg attcttggag cggtgcaaga
     1801 tattacagaa cgtaaagtag cagaccaaaa gctgcgcgat tctctacgtc aacaagaagc
     1861 catactaaac aacatccctg atatggcatg gatgaaagat ctggatagcc gttacattgc
     1921 tgtcaacgaa caatttctac gaatcagtgg gttaagagct gaagatgtcg ttggtaaaac
     1981 ggataaagac atctgggaag cttcttttgc cgaaatgtac cgccacgatg accttgaggt
     2041 gatgcaaagc ggcgttcgaa aaacagtaga agaactgcaa aaagataata caggcagaga
     2101 atattgggta gagaccacta aaaccccgat ccgcaatgaa agcggacaag taatcggcac
     2161 caccggaata gcccgtgacg tcagtgaacg gaagaagaac gaagaagcgg agcatcgtcg
     2221 ccgcgaaatg cttgaaaaag tgatcgagct tggaaaacaa gtcactgaag ccggcgactt
     2281 agataccatc ctcgataaga tctggcatgg tgttcatgac catctcgggt ttgaccgttt
     2341 gggaatcttt ttatacaacc ccgaacttgc ctccatggat agtaccattg gtacagatcg
     2401 tgatggaaat aaagtagcaa cctatggaaa ctcgttctcg ctgacaaaat ggtctacatt
     2461 ccaattactt ttagaaaaac cagatggctt gtattccact aaaaattacg atgtagaaaa
     2521 tgacattccg cctgaaaacg aaatgtatgg tgttaagcat tatgccgccg tagcggtttg
     2581 ggcaggtaat aaaccagtgg ctgctattac ggtggatcag ctcattacac atcgacctat
     2641 tacgccagaa cagttggaag cgttacgtct gtttgctggg tatgccggct tgggtattga
     2701 aaatgcccgt ttgaatatgg c
//
LOCUS       DAOREF010000401         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26115_length_2721_cov_3.501500, whole genome shotgun sequence.
ACCESSION   DAOREF010000401 DAOREF010000000
VERSION     DAOREF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26115_length_2721_cov_3.501500"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..657
                     /locus_tag="PKI33_09020"
     CDS             127..657
                     /locus_tag="PKI33_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37192.1"
                     /translation="MTTKLPQLKQDFVESLSQISRFWGFPKGMGAIFAVLYLSPTPLA
                     LDEITVQTGLTKGAVSTEVRTLARMGLVHRSTKLGDRKDYYEAETDFYTSIRSILKER
                     QNSEFDRALRGVRSSLEQLESGAVTGSKEDIAFLRERIKALQDFFDAIDSLTNAVIKL
                     EKLGLSNVSKILNVLK"
     gene            670..1023
                     /locus_tag="PKI33_09025"
     CDS             670..1023
                     /locus_tag="PKI33_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37193.1"
                     /translation="MNTIISFLIQMLLTITLTTIIVSYIRPYLRKVLIDLCGTEERAQ
                     FWTVFSNVLLIGLPSILALNYKPQANTLEELFFEVASRLSGTLAGFLFGLVCVGIVVS
                     VFALFAPRPVKAETK"
     gene            1020..1928
                     /locus_tag="PKI33_09030"
     CDS             1020..1928
                     /locus_tag="PKI33_09030"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNM37194.1"
                     /translation="MKIAVTGAFSYSGKYIAKRLLEKGEEVITLTNHPNRPNPFNGKI
                     QAFPLNFANETELAQSLQGVDVLVNTYWVRFDKGANTQPQAVENTKALVRAAVKAGVK
                     RIVHISIANPSADSHLPYYWGKAANEKAVTESGMSYAILRPTVLVGGGEDILINNIAF
                     LLKRFPIFFVPGDGQYGIQPVHVEDLAELAVEGVYSKENYVIDAVGPDSYTFKGLIEL
                     VRDTVGAKSLLISMPPRLALLAAQFLSLFVNDVILTPEEVDGLMGNLLVSKEPAHGKT
                     LFKNWLKENREIVGATYASELKKHYV"
     gene            complement(1945..2526)
                     /locus_tag="PKI33_09035"
     CDS             complement(1945..2526)
                     /locus_tag="PKI33_09035"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNM37195.1"
                     /translation="MTEDKSSRLPYRLREPFLSNTEAALFRLLTDLMGDRYMICPKVA
                     LTDLFMIVRPNENVHFFNKIFRKHVDFLLCDPKTFTPTFGVEIVKSIAKDGMREADKF
                     LAELFTDAGIPLVHIPTNDKYEPADLIALFQLAVLKVGKNIPVEVAMDSVPSCPVCGK
                     MMVLRMHRNGNSAGKKYYGCIDNPTCAGKVAID"
BASE COUNT          741 a          695 c          582 g          703 t
ORIGIN      
        1 gttttacgcc caaccctaat caaaaccgaa accgccttcg aaggcggttt ttttgtcctt
       61 gacaagctca tcaaacggtc atatactgcg tttagtaatt actaaacgtt ctaaaacgag
      121 gaatatatga ccacgaaact cccccaactc aaacaagatt ttgtcgaaag cctgagccag
      181 atcagtcgct tttggggctt tcccaaaggg atgggcgcaa tcttcgccgt cttatatctc
      241 tcccctactc cgctggcact ggatgaaatt acggttcaga ccggactcac caagggtgcc
      301 gtcagcaccg aggtacgaac cctagcgcgc atgggtttgg tccaccgctc tacgaaactt
      361 ggtgatcgca aagattacta cgaggctgaa actgatttct atacctcaat ccgctccatt
      421 ctgaaagaac gtcagaacag cgaatttgac cgggcactac gcggagtacg ctcttccctt
      481 gaacagcttg aatctggcgc tgtcactgga agcaaagaag acattgcttt tctccgtgaa
      541 cgcatcaaag ccttacaaga tttttttgat gccatagaca gcctaaccaa cgccgtcatc
      601 aagctggaaa aactaggtct ttctaatgtc tctaaaattc ttaatgtctt gaaataaaag
      661 gaaataacca tgaatacgat tataagtttt ctcattcaaa tgctactcac tattaccctc
      721 accactatta tcgtgagcta catccgtcca tatttacgca aagtactcat tgacctatgc
      781 ggcaccgaag aacgcgcgca attctggacc gtcttctcca atgttctgct gatcggtctg
      841 ccgagcatcc ttgctttgaa ctacaaaccc caagccaaca ccttggaaga actgttcttt
      901 gaagttgcca gccgcctcag cggcacctta gccgggttct tgttcgggct cgtttgtgta
      961 gggattgtcg tttccgtgtt cgccttattc gctcctcgcc cagtgaaagc agagacaaaa
     1021 tgaaaattgc agtgacaggc gcttttagct actcaggcaa atacattgcc aaacgtttac
     1081 ttgaaaaggg tgaagaggtc attactctca ccaatcaccc caaccgtccc aatcctttca
     1141 atgggaagat acaagccttc ccgctgaact ttgccaacga aactgaatta gcccaaagct
     1201 tgcaaggcgt agatgtcttg gtcaacacat actgggtgcg ctttgataaa ggggcgaata
     1261 cacagcctca agcggttgaa aacacaaagg cattggttag agccgcggtc aaagctggcg
     1321 tcaaacgcat cgttcatatc agcatcgcaa acccatctgc agattcgcat ctgccttatt
     1381 attggggaaa agctgccaac gaaaaagctg tgaccgagtc tggaatgtcc tatgccatcc
     1441 tgcgcccaac ggtgctagtc ggcggaggcg aagatatcct catcaacaat attgcgtttc
     1501 tcttgaagcg cttccccatt ttctttgtcc ccggcgatgg gcagtatggt attcaaccag
     1561 tgcatgtcga agacctcgcg gagttggctg ttgaaggggt ttatagcaaa gaaaattatg
     1621 tgattgacgc tgtgggtcca gattcgtaca cattcaaagg actgatcgaa ttagtcagag
     1681 atacggttgg cgcaaagagt ttattaatct ccatgccgcc gcgcttggct ctgctcgccg
     1741 ctcaattctt gagtcttttc gtcaatgacg tgattctcac ccctgaagaa gtggatggat
     1801 taatgggaaa tctgctggtt tccaaagagc cagcgcatgg caaaaccttg ttcaaaaatt
     1861 ggctaaagga aaatcgtgag atagttggtg caacctatgc atctgaatta aaaaagcatt
     1921 acgtctaata cccccaaaag caacctaatc gatggctact ttgcctgcac aggttgggtt
     1981 atcgatgcat ccgtaatatt tttttccggc gctgttgcca ttacgatgca tacgcaaaac
     2041 catcatcttg ccgcaaactg ggcaacttgg caccgaatcc atcgcaacct ctactggaat
     2101 attcttcccc actttcaaca ctgccaactg gaacaatgca atcaaatctg ctggttcata
     2161 tttatcgttc gtaggaatgt gaaccagcgg aattccagca tcggtaaaaa gttctgctaa
     2221 aaatttatcg gcttcgcgca tgccatcttt tgcaatcgac ttgacgatct ccaccccaaa
     2281 ggtgggagtg aaggtcttcg ggtcgcacaa tagaaaatca acatgcttgc ggaaaatctt
     2341 attaaaaaag tgaacgtttt cgttcgggcg tacgatcatg aacaagtctg tcagagcaac
     2401 cttagggcaa atcatataac gatcacccat cagatccgtc aacaaacgaa aaagagccgc
     2461 ttccgtattg cttaggaagg gctctcgaag acggtaagga agtcggctgc ttttatcctc
     2521 ggtcataatt cgattatacg ttaggtttga atctacgtac agaaatttga cctactttcc
     2581 gcgcatcaat ttcataacat ctggcttgag tgcggcgtta caagccaacc cttcaccgtg
     2641 aatgtgtccc tccgttccat cccacgcccc gaagaatcct cccgcctcct tcatgatcac
     2701 cgggtatgga ccgcaatcat a
//
LOCUS       DAOREF010000402         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26190_length_2716_cov_5.235250, whole genome shotgun sequence.
ACCESSION   DAOREF010000402 DAOREF010000000
VERSION     DAOREF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26190_length_2716_cov_5.235250"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKI33_09040"
     CDS             complement(<1..492)
                     /locus_tag="PKI33_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37196.1"
                     /translation="MESQPYIPGFKPVDVGPLSRFLPALEDGTISGWLSGQTLAGNWL
                     LDPFGFSPKLVLEAARSGYRVLVTANNPITRFLLEVFANPPPQADFVAALADLGAVKK
                     GDERLEQHLQSLYLTQCEKCEKQIYAQAFLWRKGEDVPYAKLYECPHCKDSGERIATD
                     SDKE"
     gene            complement(494..1462)
                     /locus_tag="PKI33_09045"
     CDS             complement(494..1462)
                     /locus_tag="PKI33_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM37197.1"
                     /translation="MNFSKFNLAVVIPAYRTEKEILVVLQGIPAFIRHIIVVDDASPD
                     SSADLVTAAAKKNRRILLVRHAKNQGVGGAMVTGFKKALELGAEIVIKLDGDGQMDPQ
                     YIPALITPLITGEADYAKGNRFRDFDALRQMPIVRRIGNLGLSFLTKAATGYWNIFDP
                     TNGYFAIRAEMLAQLSLDRIDKGYYFETSMLSRLYLRDAFVQDVTIPARYRNEVSSLS
                     IRRVLFEFPYKLTRTLIKRIILKYFIFDFSMMSIYLLTGIPLLLFGLIFGITKWIQYA
                     ELGIAAPTGTVILPTLSVILAIQILLSAIEIDLNAAPRKAISKALV"
     gene            1515..2663
                     /locus_tag="PKI33_09050"
     CDS             1515..2663
                     /locus_tag="PKI33_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37198.1"
                     /translation="MATGERILIVESDPDIADLIGRQALKPLGYQVTVVGDAGSAIKF
                     AAQTPPDLILANINLPGLSGKDLLAALNSQNIKSPVIVIAEKGQEPDAIQAFRLGAMD
                     VMFWPLRDAEAVSIVERAMRQTQEARERQKLDRQLKATNDELQRRLRDMTTILTIAKA
                     VTSVNDQRYLFDRILESALQLGEAEMCWLMLREDKGNLFLLRAQRGLPEAWAKKMNQP
                     MDDGISSLVALSGESLFMNGSALQKFKIASLGKSAGVVPIKIKNEVIGLMIVVRKGEK
                     EFVKDSQTMLEAMADYASISLVNARLFRALEQTAENARTGEKARYASLETLRKSIRTE
                     VESAVYPLNLVLTEMPGALNPEQKKALETVKTALQKLSHASENTVTSK"
BASE COUNT          733 a          632 c          698 g          653 t
ORIGIN      
        1 ttctttatct gagtcagtgg caatccgctc gccagagtct ttgcaatgtg ggcattcata
       61 cagttttgca tagggcacat cctcgccttt gcgccagagg aaagcctgtg cataaatctg
      121 tttctcgcat ttttcacatt gcgtaaggta aagggattgt aaatgttgtt caagtcgttc
      181 gtcccctttt ttcacggcgc caagatcggc aagcgcagcg acaaagtccg cttgcggtgg
      241 cgggttggca aacacttcca gcaaaaagcg tgtgatggga ttatttgccg tgacgagcac
      301 acggtatcca ctgcgggcgg cttcgagcac aagctttggg gagaatccga atgggtcgag
      361 caaccagttt ccggctagag tctgacccga gagccagcct gaaatggtgc catcttccag
      421 cgcaggtaaa aagcgcgaga gtggaccaac gtctacgggt ttgaatccgg gaatgtaagg
      481 ttgagattcc atatcacacc agcgctttgc tgatggcttt gcgcggagcg gcgttgaggt
      541 caatttcgat ggcagaaagc aaaatttgaa tggcgagaat caccgagagc gttggcaaaa
      601 tgaccgtgcc tgtgggcgca gcgattccta actcggcata ttgaatccat ttggtgatgc
      661 caaagatcaa cccgaaaagc aacaacggaa tgccggttag taaatagatc gacatcatcg
      721 aaaaatcaaa gatgaagtat ttgaggatga tgcgtttgat gagagtgcgg gtgagcttat
      781 atggaaattc aaacagcaca cgcctgatcg aaaggcttga aacttcattt cggtagcgtg
      841 caggaatggt cacatcttgc acaaaggcat cgcgcaggta taagcgcgag agcatagacg
      901 tttcaaagta atagccttta tcaatgcggt cgagcgagag ttgcgccagc atttcagcac
      961 gaatggcgaa gtacccattg gtggggtcaa aaatattcca ataccccgtg gcagctttgg
     1021 tgagaaagct caagccaagg ttgccgatgc gccgtacgat tggcatttgc cttaatgcat
     1081 caaaatcgcg aaagcgattg cctttggcat aatctgcttc accggtaatg agtggcgtaa
     1141 tcagcgcggg gatgtattgt gggtccattt gcccatcgcc atcgagtttt attacaattt
     1201 cggcaccaag ttctaaggct ttcttaaaac ctgttaccat cgcgccacct acaccttgat
     1261 tttttgcgtg acggacgagg aggatgcgtc gattcttttt ggcggcagca gtgactaggt
     1321 ccgcgctaga atcgggcgaa gcatcatcca ctacaataat gtggcgaatg aaagcaggaa
     1381 ttccctgtag aaccacgagg atttcttttt cggttcgata ggcaggtatc acaacagcaa
     1441 ggttgaattt cgaaaaattc atcttcaaga ttgtaatcga aaaggtgata gaatcttttg
     1501 aaggagattt acacgtggct accggggaac gcatcctcat tgttgaatca gacccagata
     1561 ttgccgattt gatcggaaga caggcgctta aaccactcgg ataccaagta accgtggtgg
     1621 gcgatgccgg ctccgcgatc aaattcgcgg ctcagactcc gccagacctg atcctcgcca
     1681 atatcaattt acccggcttg agcggaaaag acctactcgc cgcgttgaac tctcagaaca
     1741 taaaatcccc cgtcattgtt atcgctgaaa aaggacaaga accagacgcc attcaagctt
     1801 ttcgccttgg cgccatggac gtgatgttct ggcccctgcg cgatgcagaa gccgtctcta
     1861 ttgtagagcg tgccatgcgt cagacgcagg aggcacgtga acgccaaaaa cttgaccgcc
     1921 aattaaaagc gactaatgac gaattacaac gtcgcctgcg cgatatgacg accattctaa
     1981 ccattgctaa ggctgtcact tcggtcaacg accaacgcta tctgtttgac cgcatccttg
     2041 agagtgcctt gcaacttggc gaagccgaga tgtgttggct gatgctgcgt gaagataaag
     2101 gcaatctctt cttgctgcgc gcccaacgag ggctccctga agcttgggca aagaagatga
     2161 atcaaccgat ggatgatggc attagttcat tggtggcgct ttccggcgaa agtctgttca
     2221 tgaacggctc cgcgcttcag aaatttaaga ttgcatcgtt aggcaagtct gctggcgttg
     2281 tgcctatcaa gatcaagaat gaagttattg ggttgatgat cgttgtacgc aagggtgaaa
     2341 aggaatttgt gaaagactca caaaccatgc ttgaagccat ggcagactat gcatcgattt
     2401 cattggtgaa tgcccgcttg ttccgtgctc ttgaacaaac cgccgaaaat gctcgcactg
     2461 gtgaaaaagc ccgttatgct tctttggaga cactgcgcaa atccattcgc acagaagtgg
     2521 aatctgcggt ctatccgctc aacctggtgt tgacagaaat gcccggcgca ctaaacccag
     2581 aacaaaagaa agcattagaa accgtgaaga ccgctctgca aaaactttct catgcttctg
     2641 agaatacagt cacatccaag taaggatata caaagaaagt aatgtcaaaa caagacctga
     2701 ttttgctggc attgga
//
LOCUS       DAOREF010000403         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26193_length_2716_cov_3.720406, whole genome shotgun sequence.
ACCESSION   DAOREF010000403 DAOREF010000000
VERSION     DAOREF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26193_length_2716_cov_3.720406"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="PKI33_09055"
     CDS             complement(<1..1900)
                     /locus_tag="PKI33_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM37199.1"
                     /translation="MLDLLMSVCGTALLAFDETGKIIRANESVAEMFGCSVEELLKKK
                     INSLLPKATARRHSKLFQQFFEEAASHRKMGKYRSVIAQRKNGEEFSVEISIGKAEMD
                     GKWIAIASLRDLAMEKHNEEFFRSTAIASGENPNPVFRISPDGKIIFSNTSAEEMLKS
                     IGHHEQNIPAAWVNSLQQVIDSEIQVVSIIQHGERAYSCVFAPAPTKEYVDMYALNVT
                     DREEEKARLALSDEILNSIGNLVMVANSNAEIVYISPSVINLLGYRPDEILGEGWWEI
                     ERISGGDVQVEKEYIRKAAAGKIDVDGKPYEHHVRHKDGSWRWLMLSDTKGPRDLVIG
                     IGYDITPIKAAEEELERQRDFARTLTNQMGQGLTVTDENGCFEFVNPSYAQMLDYEPS
                     ELIGKTPYDFTLPEDHWNLLNAQVKRSLGSVTTYETRLLGRNNKIVYALITGVPRSVN
                     GNYKGAITVVTDLTERLTMEKQLRQYADEIQQANIQLADARDRALEASYLKSAFLATM
                     SHEIRTPMNAILGMSELLLDTELNEEQREFANVIEASTQNLLAILNDILDLSKIEAGK
                     LAIHPAPFNPASVIMETMKLFSPKAQQKNINVSVNVPPDAQTLVLGDAGRIRQVLSNL
                     LSNAIKFTE"
     gene            complement(1893..>2716)
                     /locus_tag="PKI33_09060"
     CDS             complement(1893..>2716)
                     /locus_tag="PKI33_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNM37200.1"
                     /translation="KVLAISYQVEMWSRLLVSILLVALGLSVNGAVIGISLSFVFTWL
                     VARSAVVGLDSQENISKEMRAELLAFAAPVLVSQLGQILINNSDVILVKRFFETSEAG
                     KYAALALIGRIVFFATWSVVTTMFPIVAQKQKMGEPHAHLLWVSLGIVAGVSLPIVLL
                     TWLVPDWVVLVLFGKKYLNISGLLWMYALATSLYAMANVVINYRLSLGMGRETGFAIA
                     AGLAQVFGIIYFHNSLAQVVYVQVVVMGVLFLALMWHNFLHEKARTLLQAVTHHA"
BASE COUNT          788 a          681 c          525 g          722 t
ORIGIN      
        1 gttctgtgaa tttgatagcg ttgctcaaga ggttgctcaa tacctggcgg attcgccctg
       61 catctcccaa gactagagtt tgagcatcag gcggaacatt aacggaaaca ttgatattct
      121 tttgctgtgc tttagggcta aagagcttca tggtttccat gattactgag gcaggattaa
      181 acggtgcagg atggatcgcc aactttccag cttcgatctt ggaaagatct aaaatgtcat
      241 ttaagatcgc caataaattc tgggtagaag cttcaatgac attcgcaaat tctctttgct
      301 cttcatttag ttcagtatct aataacagtt cactcatccc caaaatagca ttcatcggtg
      361 ttcgaatttc gtgactcata gttgcaagaa aagcgctttt taaataagaa gcttcaagtg
      421 cccggtcgcg tgcatctgcc aattgaatat ttgcctgctg aatttcatca gcatattggc
      481 gtaattgttt ttccatggtt aaccgttccg ttaaatcggt tactacggtg attgcacctt
      541 tataatttcc attcacgctg cgtggcacac cggtgatcag agcataaaca atcttattgt
      601 tacgtccgag caaacgggtc tcatatgttg ttacactgcc caaagaacgt tttacttgag
      661 catttaacaa attccagtga tcctctggaa gggtaaagtc atagggtgtt ttaccgatca
      721 attctgatgg ctcataatcc aacatctgtg cataagaagg attaacaaat tcgaaacagc
      781 cattttcgtc tgtaacagtt aatccctgtc ccatttgatt ggtaagggtt cgagcaaaat
      841 cacgttgacg ttcgagttcc tcttccgctg ctttgatggg ggtaatgtca tacccaattc
      901 cgatcacgag gtctcgggga cctttcgtgt cagacaacat caaccaacgc catgaaccgt
      961 ctttatggcg tacatgatgc tcatacggtt tgccgtctac atcaatctta ccggctgctg
     1021 ccttacggat atattccttc tctacttgca catctccccc agagatgcgt tcaatctccc
     1081 accagccctc tcctaaaatc tcatcaggac gatacccaag aaggtttatc acagaagggc
     1141 tgatatagac gatctcagcg ttgctatttg caaccataac caaattgccg attgaattca
     1201 atatctcgtc agataaagcg aggcgtgctt tctcttcttc ccgatctgta acatttagag
     1261 catacatatc cacatattct tttgtgggcg ctggagcaaa aacgcaagaa tatgcccttt
     1321 ccccatgctg aataatagaa accacttgaa tttcactatc gatcacctgt tgcagggaat
     1381 tgacccaagc agcagggata ttctgctcat gatgcccaat gctctttaac atctcttcag
     1441 cactggtatt cgaaaagata atcttcccat cagggctgat acggaaaaca gggtttgggt
     1501 tctcacccga agcaattgcc gtggagcgga aaaattcttc attatgtttt tccatggcaa
     1561 ggtctcgcaa tgaagcaata gcgatccatt taccatccat ctcagctttg ccgatagaaa
     1621 tctccactga gaattcttca ccatttttgc gctgagcgat tacagaacga tatttcccca
     1681 tctttcgatg agaggcagcc tcttcgaaaa attgttgaaa gagtttggaa tgcctacgtg
     1741 cagtggcttt gggcaggagc gaatttatct ttttctttag caattcctct accgaacagc
     1801 caaacatctc agcaacagac tcattggcac ggatgatctt gccagtttcg tcaaatgcca
     1861 acagggcagt cccgcaaaca gacatcaaga gatcaagcat gatgtgtcac cgcctgcaat
     1921 agggtacgtg ctttttcatg caaaaagtta tgccacatga gcgcaagaaa cagcacaccc
     1981 attaccacta cttgcacata cacaacttgt gccaaggaat tatgaaaata aataataccg
     2041 aatacttggg caagccctgc cgctatggca aagccagtct cacgccccat tcccaatgac
     2101 aatcgataat taatcaccac atttgccatg gcgtacaatg aagttgccaa tgcatacatc
     2161 cacaacaagc cagagatatt caaatacttt ttaccgaaca aaaccaaaac cacccaatct
     2221 ggcacaagcc atgtcaacaa cacaattggc aaagaaacac ctgccacaat tcccagtgac
     2281 acccataaca agtgagcatg tggctccccc atcttttgtt tttgcgccac aatagggaac
     2341 atggtcgtta caactgacca agtagcaaaa aacacaatac gcccaatcag cgccagcgct
     2401 gcatatttcc cagcttcact cgtctcaaaa aagcgcttca ccaagatcac atcgctattg
     2461 ttgataagaa tttgccctaa ctgagaaacc agaacgggtg cggcaaacgc cagcaattcg
     2521 gcacgcattt ctttggaaat attttcttgt gaatccagcc cgactactgc agagcgcgcc
     2581 accagccatg tgaatacgaa agacaaacta atgccgatca cagcaccatt gaccgaaagc
     2641 cccaaggcaa ccagaaggat actgaccaac aaacggctcc acatctcgac ttgataactg
     2701 attgccaaaa ccttga
//
LOCUS       DAOREF010000404         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26264_length_2711_cov_4.068524, whole genome shotgun sequence.
ACCESSION   DAOREF010000404 DAOREF010000000
VERSION     DAOREF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26264_length_2711_cov_4.068524"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKI33_09065"
     CDS             <1..248
                     /locus_tag="PKI33_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37201.1"
                     /translation="WRYLVGRGFHIIFFENRNTTIPNIIRKDLKEIPSWLKIAMLQND
                     QNIFFIKLTPAEYASTHLFECKQTIPPSWDVFEISSP"
     gene            complement(299..1915)
                     /locus_tag="PKI33_09070"
     CDS             complement(299..1915)
                     /locus_tag="PKI33_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNM37202.1"
                     /translation="MKKFLMIVSVLTVLTLALGACGPTATSTEAPQTEETQPSGQSAT
                     ADGSEQPTEAPTDVAIKTVDLVGPPMEVGSKYMFVDGTVLVAVPGGEFTMGYNFADNP
                     ERKITVGDFWIYSTKVTNRQYAMCVAAGKCTPPDPEKAPNFGTVEFTNFPVTGVTHTQ
                     AAEYCGFVKGHLPTEAQWEKAARGPEGNIFPWGNGAPTCSLTNYALCKGQSTSVNDYP
                     DGKSFYEAWDMSGNVREWVADWYSPVYNVDSPVADPLGPELGEKRSVRGSSYADSANM
                     TIAAHRFSLDPNESSEDVGFRCVIGEPDPSTLFAPWCELVGYAGTGPDGTPGSCTPEV
                     KCNGVNISVSQFCEPGKESYSVVTLEVVDTPPDSWEANPPAGCSVIDETAEKKKYLCT
                     VDSSASATGSCVDTVQCAATCPMNYNKVGDKCVWDGSGTSGTDCLPGATYDPLTQCCT
                     ATPGMGVDFGLCPAGFYNVNGVCVPNPKGTVKSVTSVVDVIENCAVPTDDPNDPGDEP
                     DNPGGGGCTPPSKPCQTWETYDPATCTCIP"
     gene            complement(2150..>2711)
                     /locus_tag="PKI33_09075"
     CDS             complement(2150..>2711)
                     /locus_tag="PKI33_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37203.1"
                     /translation="YTLDPSTGACAYTPILSQPTAGGCPLGYVTVQRGDQQVCAVGPA
                     ADNSCPIGLYFDDLAGMCVPPNGETNAPYGIDNTTLASQTYAGCTAGYSYNDSFQCCQ
                     PSAGAAYPSCEPGFTFDSNSAACVPVLEEALNGTGCVAARVNTVKCVQLEDKVCAPIV
                     SESRCVANLNCTWNEKDATCDPRVKP"
BASE COUNT          729 a          689 c          606 g          687 t
ORIGIN      
        1 ggtggagata tcttgttggc agagggtttc acatcatttt ttttgaaaat cgcaacacaa
       61 caattccaaa tatcattaga aaagatttaa aagaaatccc aagctggtta aaaatagcga
      121 tgttacaaaa cgaccagaat atatttttta ttaagctcac tcctgcagaa tatgcttcta
      181 ctcatttatt tgaatgcaaa cagacgattc caccttcatg ggatgttttc gaaatatcta
      241 gtccatagaa aaaaagctct caacaaatgc tgagagcttt tttctttatt caattcaatt
      301 atggaataca agtacaggtt gcagggtcat acgtttccca agtttgacaa ggtttagatg
      361 gtggtgtaca tccaccaccg cctggattgt ccggttcatc accgggatcg ttggggtcat
      421 ccgtaggcac ggcacaattc tctatcacat cgacaacact ggttacactc ttaacagtac
      481 ctttcgggtt cggcacacat acaccattca cgttatagaa accagctggg cacaagccaa
      541 agtccacgcc cataccgggg gtcgctgtac aacactgagt caacgggtcg taggtcgcgc
      601 cggggagaca atccgtacca ctggttccac tgccatccca aacgcacttg tcacccactt
      661 tgttgtaatt catggggcag gtcgcggcgc actgaacggt gtccacacaa gagcctgtag
      721 cagaggcaga gctatcaacg gtacagaggt atttcttctt ctctgcggtt tcatcgatca
      781 cactacagcc cgcaggagga ttcgcttccc agctatctgg tggggtatcc acaacttcca
      841 gtgtcacaac cgagtaggat tctttgcccg gttcacaaaa ttgtgagacg gagatgttca
      901 caccattaca cttaacttcc ggcgtacaac taccgggagt gccatcgggt cccgtcccgg
      961 catacccaac caactcacac cacggagcga aaagagtaga gggatctggc tcgccaatca
     1021 cacagcggaa acccacatct tcagaagact cattcgggtc gagagagaag cggtgagccg
     1081 caatagtcat attggcactg tcagcatagc tacttccacg tacagagcgt ttttcgccta
     1141 gttcgggtcc gagtgggtca gcaacgggac tatccacgtt ataaaccggg ctgtaccaat
     1201 ctgctaccca ttcgcgcaca ttcccagaca tatcccaagc ttcgtagaag cttttgccat
     1261 ctgggtaatc attaaccgaa gtggattgcc ctttacaaag agcataattg gtcaggctgc
     1321 aggtcggtgc cccattgccc catgggaaga tattgccttc tggaccgcga gcagcttttt
     1381 cccattgcgc ttcggtagga agatgtccct ttacgaagcc gcagtattcg gcagcttgag
     1441 tgtgggtcac gcctgtaacg ggaaagttgg taaattctac agtaccgaaa ttaggcgcct
     1501 tttcagggtc tggaggtgta catttcccag cggcaacaca catggcatac tggcgattgg
     1561 tcaccttggt gctgtatatc caaaaatcac ccacggtgat cttgcgttct gggttatcag
     1621 caaagttata gcccatggta aattccccgc ccggcacagc caccagcacg gttccatcca
     1681 cgaacatgta tttagagccc acttccatgg gcggtcctac caagtctacg gttttgatgg
     1741 caacatccgt aggtgcttca gtcggctgtt cgctaccatc tgctgtagca ctctgcccag
     1801 aaggctgagt ttcttcagtc tgtggcgctt cggtgctcgt ggcggtaggt ccacatgccc
     1861 caagtgccag ggtcaacaca gttaggaccg aaacaatcat taaaaatttc ttcatgtttc
     1921 actctctcct tttctaaaaa ttcatgattt caaaatatga aaacatatct tcagtataat
     1981 gatcatggtc gacgaaagga acttaagagc atcacccatt tgtcttattc aacgctccac
     2041 catttagtac tgggtatagt actgggtaac aatatatatt ttgggctatg aatcaataaa
     2101 cccatgaagt attcatgggt ttattgattc atagttctag agttctaaat taaggcttga
     2161 ctcttgggtc acaagtagcg tccttttcat tccaagtaca attcaaattc gctacacaac
     2221 gagattcaga gacaattgga gcacaaacct tatcttcaag ctgtacacac ttcaccgtgt
     2281 tgacacgggc agccacacaa ccagttccat tcaatgcttc ttccaaaaca ggtacacaag
     2341 ccgcagagtt ggaatcaaag gtaaaacctg gttcgcaact tgggtaagca gcccctgccg
     2401 aaggctgaca acactggaag ctatcgttgt agctataccc agcagtacaa cctgcatagg
     2461 tctgtgaagc caaagtggtg ttatcaatac catatggtgc attcgtctct ccatttggcg
     2521 gtacacacat tccagccaaa tcgtcaaaat acaaaccaat cgggcaggag ttgtctgcgg
     2581 caggacccac cgcacagact tgctggtcgc cgcgttggac agttacatac ccaagcggac
     2641 atccgcctgc cgtaggttgc gaaaggatcg gggtataagc acatgcacct gtagaaggat
     2701 caagcgtata c
//
LOCUS       DAOREF010000405         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26318_length_2708_cov_3.307199, whole genome shotgun sequence.
ACCESSION   DAOREF010000405 DAOREF010000000
VERSION     DAOREF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26318_length_2708_cov_3.307199"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKI33_09080"
     CDS             <1..451
                     /locus_tag="PKI33_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37204.1"
                     /translation="FVTLAPTSTFEGTPAIVNIALNMGAGGAGFSANLYAQEVYTGRT
                     VGIFMSAGMHTAPLLPTSPPLDMMVPAPGTYVFYARLSNDPMDYHYGYTQCQVPTDCV
                     GSPLIAMKVEPGQRYNILIADRKALLPEINERGLPVTVPYVLVESNP"
     gene            448..1875
                     /locus_tag="PKI33_09085"
     CDS             448..1875
                     /locus_tag="PKI33_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNM37205.1"
                     /translation="MKTLLIPLDTNATKALYLQVADYITEAARSGQLKPGTQLPPSRT
                     LAEQNNVHRSTIINAYQELKARGVLASKQGSGTYIEKGLSENTVSIQKFKPESLPTDE
                     LIAEIRRMHWSEGMISLGYGPPSDELMPVEDFDRSRQRVLRRDGKKTANYEHPQGYYP
                     LRQAICADLVKQGIQADPDEILITNGVLEGVALVSRAMAAPGDAVLTELPQYFGNWTN
                     FSYLGLNVRGFDLLESGPDFASLEAQLQDENDRPRFIFVTADHQNPLGTCWTMPARHE
                     FLKMMNKYDVPVVEDATYRDLTYDGMPHTPLRALDPDVIYVGTFSHSLMPGLRIGFVL
                     CRGRLREHIERLKAISSGPVETLNQRSLADFLATGEYERHLDRIIPVYRARRDALLNA
                     MQTYFPPELRWTKPTGGFFVWIWLPDPISAIDLFYRSVKKGISIAPANIFYPGPCPQN
                     AFRLAFSHYPEDVLTRAVMEIGKLL"
     gene            complement(1933..>2708)
                     /locus_tag="PKI33_09090"
     CDS             complement(1933..>2708)
                     /locus_tag="PKI33_09090"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNM37206.1"
                     /translation="AIVLGGSRARGTHTPSSDIDLGIYYDPSQPMDLSALGNIATRLD
                     DEHRTDVITGFGGWGPWINGGGWLKVQSLPVDFLYKDLGRIKSYIDACLNGKVEIFYQ
                     GGHPHGFVTSIYLGEVALCKPLYDPEGIIAELKDKVTPYPEALQKAILASFAWEIDFS
                     AGIAKKSIERADVNYAAGCCFRAVMCMLQVLFALNKEYWMNEKGAINITDRFAIKPAD
                     FRKRVAEVYALLEASPEALEKAIYLLKGLSEDVYGLLPK"
BASE COUNT          692 a          765 c          613 g          638 t
ORIGIN      
        1 gtttgtaacc ctcgccccaa cctccacctt cgaagggaca cctgctattg tcaacattgc
       61 cctgaacatg ggcgcaggcg gcgcgggttt ctctgctaat ttatatgcac aggaagtgta
      121 tacgggtaga actgtcggca tctttatgtc ggcagggatg cacacagcgc cactattacc
      181 cacctcgcct ccattggata tgatggtacc cgcgccggga acatacgtct tctacgcccg
      241 cctcagcaat gacccaatgg actatcatta cggctatacc caatgccaag tcccaaccga
      301 ttgtgtgggc agcccgttga tcgccatgaa agtggagccg ggtcagcgct acaacattct
      361 gatcgcagac cgcaaagctc tgctacctga aatcaacgaa cgcggattgc cagtgaccgt
      421 tccatacgta ctggtagaat ccaacccgtg aaaacattgc tcatcccttt agatacaaac
      481 gccaccaaag cgctttacct gcaagttgca gattacatca ccgaagccgc ccgcagcgga
      541 cagttaaagc ccggcacaca gctaccgcct tcgcgcacct tggctgaaca gaataacgtc
      601 caccgctcaa ctattatcaa tgcctatcag gaactcaaag cgcgcggtgt gctggcgtcc
      661 aaacaaggaa gcgggactta catcgaaaag ggattgagcg agaacaccgt gtcgattcaa
      721 aagttcaagc cagaaagcct gcccaccgac gaactgatcg cagaaattcg ccgtatgcat
      781 tggtctgaag gcatgatctc acttgggtat ggtccgccgt cagatgagct gatgcccgtg
      841 gaggacttcg accgctcccg tcagcgggtg ttgaggcgcg atggaaaaaa gacagccaac
      901 tacgaacatc cgcaagggta ttatccactg cgccaagcca tctgcgcaga cctcgtcaag
      961 cagggcattc aagcagaccc agatgaaatc ctcatcacaa atggagtctt ggaaggtgtt
     1021 gcacttgtga gccgtgccat ggcagcaccg ggtgatgccg tgttaactga actgccacaa
     1081 tactttggca attggactaa tttttcgtac ctcggtttga atgtgcgcgg ctttgacctg
     1141 ctcgaaagcg gacccgattt tgcgtcactt gaggcgcaac ttcaagatga aaatgacaga
     1201 ccacgattca tcttcgtgac agccgatcat caaaatccgc ttggcacttg ttggacgatg
     1261 cccgcccgcc acgaatttct caagatgatg aacaaatacg atgtgcctgt cgtagaggat
     1321 gcgacctatc gcgacttgac ttacgatggc atgccccaca caccgcttcg ggctctggac
     1381 ccagacgtga tctatgtcgg gacgttttct cattcgctga tgcccggtct ccgtatcgga
     1441 ttcgtgcttt gccgtgggcg tctgcgcgaa cacattgaac gcctcaaagc catctccagc
     1501 ggacccgtcg aaacgcttaa ccaacgctcg ttggcagatt ttcttgctac gggtgaatat
     1561 gaacgtcatc ttgatcgcat tatccccgtc taccgcgcac gacgcgatgc gcttctgaac
     1621 gcgatgcaaa cctacttccc gccagaactc cgctggacga aacctactgg cggcttcttc
     1681 gtgtggatct ggttacctga ccctatttct gcaattgact tgttctatcg ttccgtgaaa
     1741 aaaggaatca gcatcgcccc cgcgaacatc ttctaccccg gtccctgccc gcaaaatgca
     1801 tttcgtttgg cattctctca ttaccctgaa gatgtgttga caagggcagt aatggagatt
     1861 gggaagttgt tgtaattaaa acaaaagacc tcggaggttt ttaaaacctc cgaggtcttt
     1921 ttaaattcaa ctttacttcg gcaacagccc gtagacatcc tcactcaaac ccttcaacaa
     1981 atagattgct ttctctagcg cttcgggaga ggcttctaat aacgcataaa cttccgcaac
     2041 acgcttacgg aaatctgcgg gtttgatggc aaaacggtct gtgatgttga tagcgccttt
     2101 ttcgttcatc cagtattctt tgttcaacgc aaacaaaacc tgcaacatgc acatcactgc
     2161 acggaagcaa caacccgccg cataatttac gtccgctcgt tcgatgcttt tcttagcaat
     2221 tcccgcagaa aaatcgatct cccaagcaaa actggcaaga atcgcctttt gcaacgcctc
     2281 cgggtacggg gtgactttat ccttcaattc tgcgatgatg ccttcggggt catacaaggg
     2341 tttacaaagc gcaacttcac ccaaataaat tgaggtcaca aatccatgcg gatgcccgcc
     2401 ttggtaaaaa atttccactt tgccattcaa acaagcatca atataggact taattctccc
     2461 caagtcttta tacaggaagt ctacaggcaa cgattgaact ttcaaccacc caccaccatt
     2521 aatccacgga ccccaaccgc cgaagccggt aatcacatcc gttcggtgtt catcatcaag
     2581 ccgtgtcgct atatttccta aagcgctcaa atccataggc tgacttgggt cgtagtaaat
     2641 tcctaagtca atatcagaag aaggggtatg cgtccctctg gcgcgcgagc cgcccaatac
     2701 aatggctt
//
LOCUS       DAOREF010000406         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26389_length_2703_cov_4.715257, whole genome shotgun sequence.
ACCESSION   DAOREF010000406 DAOREF010000000
VERSION     DAOREF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26389_length_2703_cov_4.715257"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PKI33_09095"
     CDS             <1..1577
                     /locus_tag="PKI33_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37207.1"
                     /translation="ARLDAAWFGDPAQRRVYADAQECVTLFRQYCLERNLLDFSLQME
                     VFKNHLWQNEQARSYLIQTYRHLVYDNVEEDVSSSHDILDQWVPEFESALLLYDVGAG
                     YKKFLGGDPASGYALSDRCDEVFELNESFVSSPNVIQLSKALVSAINGQGEIEADGTQ
                     PAMEFIMTHFHPEMLDEVVTKIRLLLEAGTPPSEVVILAPYLSDSLRFSISNRLDSAG
                     IPWRSNRPSRSLRDEPASKALLTLSALAHPQWNVHPSKFDVAHALMFALGTDLIRSQL
                     LTDIVYGQRELRLSSFDQINPETQERITYALGARYATLREWIETYRNEAPLPLDFFLR
                     RLFGEVLSQPDFGFHTNLDAVRIAASLVESIKKFRNAMEPLQTLNDFDLGMEYITMLQ
                     DGVIAAQYLESWRSEDKEAVLVAPAYTFLMMNRPVAFQFWLDPGSDGWSQRVSQPLTH
                     PYILSRYWDSSSGRVWLDSDEVEMEIETLSRLVGGLLARCREKVFLALSDLGAAGFEQ
                     RGALLRAFQKVLQRQQ"
     gene            1605..>2703
                     /locus_tag="PKI33_09100"
     CDS             1605..>2703
                     /locus_tag="PKI33_09100"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNM37208.1"
                     /translation="MRTSKEFKQRLTFALLFSFGLLLRVLFIPAKTLDMGSYILWYDY
                     IVKNGLGAIGSNYFGYNPPFIYLLALTTLTHSFLHPVIAIKLIPFIFDILSAILVYHI
                     VRMQEHEEKKPLLAALIFWIAPTIMINSSFWGQTDAIYTFFLLFTTFFLLKERPIFAL
                     LAFGIAFSIKAQAIFFAPFLGVLFFKKKIPFKAFLTFPVVYAISFIPAIIAGRPIATL
                     FSTYGGQTETFASASKNAANPYFFVNEQDYLTILYIGLVIAVTLLVTWVLIYSFKKYE
                     ITKPNLIITALNSLAIVPYLLPKMHDRYFYPADVFSIICAFFLPRFWPVAIAYQAISF
                     LSYLPYLFNTPAEATVSIAFVINTITIIFLIQ"
BASE COUNT          653 a          664 c          531 g          855 t
ORIGIN      
        1 gcgcgcgctt agatgccgcc tggttcggtg accctgccca gcgccgcgtg tatgctgatg
       61 cgcaagaatg tgtcaccttg ttccgacaat attgccttga acgtaatttg ctagatttct
      121 cacttcaaat ggaagtcttc aagaatcacc tctggcaaaa cgaacaagct cgttcgtatc
      181 ttattcaaac ctatcgtcat cttgtttatg acaatgtcga agaagatgtc tcgtcttctc
      241 atgatattct cgaccaatgg gtacctgaat ttgagtccgc gcttttgctt tacgacgtag
      301 gcgcaggcta taaaaaattt ctgggtggag acccagccag cggctacgcc ctgagcgaca
      361 gatgcgatga agtatttgag ttgaacgagt cctttgtctc gtcgccaaat gtgattcaac
      421 tctcaaaggc acttgtctca gctatcaatg gtcaaggtga aattgaagcg gatggcaccc
      481 aacctgcgat ggaattcatc atgacacact tccatcctga gatgttggat gaagttgtca
      541 caaaaattcg cctgttgctt gaagccggca ccccgccttc tgaagtagta attcttgcgc
      601 catatctatc agactcgcta cgattctcga tttcgaatcg tttagattct gctgggattc
      661 cctggcgcag caatcgtcca tctcgttctt tgcgcgatga acccgccagc aaagccttgc
      721 tcactttatc ggctcttgct cacccacagt ggaatgttca tccatcaaag tttgacgttg
      781 cccatgcttt gatgtttgcc cttggcacag acctcatccg ctcgcagttg ctcaccgata
      841 ttgtgtatgg tcaacgtgag ttaaggcttt caagttttga ccagatcaac cctgaaaccc
      901 aagaacgtat tacctacgct ttgggggctc gttacgcaac attgcgcgag tggattgaaa
      961 cttatcgcaa cgaagcgcca cttccgctcg attttttcct tcgccgctta tttggcgaag
     1021 tgctctcgca acctgatttt ggttttcata ccaacctcga tgcggttcgt atcgccgcca
     1081 gccttgtcga atcgatcaag aagttccgca acgccatgga gccgcttcaa actctaaacg
     1141 actttgacct tggcatggaa tacatcacca tgctgcaaga tggggtcatt gcagcccaat
     1201 accttgaatc atggcgcagt gaagataaag aagccgtttt ggtcgcccca gcatatacct
     1261 tcctaatgat gaaccgcccc gtcgccttcc aattctggct ggaccctggt tctgatggct
     1321 ggtcacagcg cgtgtcacag ccgctcaccc atccctatat cctttcgcgc tattgggatt
     1381 cgtcttctgg gcgcgtttgg cttgactcgg atgaagtcga aatggagatt gaaaccctat
     1441 cccgcctcgt tggcggtttg ctcgcccgtt gtcgtgaaaa ggtatttttg gcattgtctg
     1501 atcttggtgc cgcagggttt gaacaacgtg gcgcgttatt acgggcattt cagaaggtgt
     1561 tacaacgaca acaataaact ttatttcctt ttatgaggct tttcatgcga acatctaaag
     1621 aattcaaaca gcgcttaacc tttgcgttac ttttttcgtt cggactcttg ctaagggtat
     1681 tgttcattcc tgccaaaaca ctcgacatgg gctcatatat cctttggtat gactacatag
     1741 tcaaaaatgg gcttggggct attggtagta attattttgg ctacaatccg ccatttattt
     1801 atttgttggc acttacaaca cttactcatt catttctaca tcccgttatt gccattaagc
     1861 ttatcccatt tatattcgat attctcagtg caatacttgt ttatcatatt gtccgcatgc
     1921 aagaacacga agagaaaaaa cctttgcttg cagccctcat tttctggatt gcaccaacta
     1981 ttatgattaa cagtagcttt tgggggcaaa ccgatgcgat ttacactttc ttccttttat
     2041 tcacaacatt ttttcttctc aaagaaagac ctattttcgc tctccttgca ttcgggatag
     2101 ccttctcaat taaggcgcaa gccatttttt ttgctccctt ccttggtgtt ctcttcttta
     2161 aaaagaaaat tccttttaag gcatttctta ctttccctgt tgtatatgct atttctttta
     2221 tacctgctat cattgcagga agacctattg caacattatt ttcaacatat ggcggacaaa
     2281 cagagacttt cgcctctgcg tcgaaaaatg cggcgaaccc atattttttt gttaacgagc
     2341 aagactactt aactattctt tatattggac ttgttatcgc tgtaacactt ttagtgacat
     2401 gggtattaat ttatagcttc aaaaaatacg agatcacaaa accaaatttg atcattaccg
     2461 cactgaactc acttgcaata gtcccatatc tcttacccaa aatgcatgat cgttattttt
     2521 atccagcaga tgtattctca atcatttgtg cattcttctt gccaagattt tggcctgtag
     2581 ctattgctta tcaggcaata tccttcctct cttatctgcc atatttgttc aacaccccag
     2641 cagaggctac agtttcgatt gcatttgtta ttaacactat tactattatt ttcctaattc
     2701 aaa
//
LOCUS       DAOREF010000407         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26434_length_2700_cov_4.592439, whole genome shotgun sequence.
ACCESSION   DAOREF010000407 DAOREF010000000
VERSION     DAOREF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26434_length_2700_cov_4.592439"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..2385
                     /locus_tag="PKI33_09105"
     CDS             148..2385
                     /locus_tag="PKI33_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNM37209.1"
                     /translation="MTLDKGTLIHNRYRIVDILGQGGMGSVYRAIDENLGVAVALKEN
                     LFTTDEYARQFRLEAVILANLRHPNLPRVSDHFVLGDLGQYLVMDFIEGEDLRYRMER
                     LGMLEEEDAVHIGAAMCDALAYLHTRKPPILHRDIKPGNVKISPDGHIYLVDFGLAKV
                     YQSASQATTTGARAMTPGYSPPEQYGTARTDPRTDIYSLGATLYAALTGVIPEDGLAR
                     AMDNAQLTPLRKRNSKVSRKLAASIEKAMAVDPSDRFQTAEEFKAGLLSSKSMTQQAP
                     GSYKVIPPPPDAVAASSHTHEVTVVDEALPVDGKKDRKALVGPVPAAGSNAHEDQPFV
                     SPLKRQKERERKRRMALWRFALFAGILLLVLGSVVFFVPGVLPADVRAMVPFIGATAT
                     PTFTLTPVPTQTLPATFTATLEPTSTPTLRPTNTPTATFTPTALPGETITITPVASSG
                     GTEVFMGGGAGQIAFASDRTGSPQIFLVDLRGESAFQVTNMQGGACQPAWSPDGLRIA
                     FISPCKGNEDIYTNTSLYIINADGTGLATLDASPGGNFDPAWSPDGKIIAYTSARTGQ
                     MEIFMLPVDDPASITQITSGAAKVESRMPTWSPDGSKLVYVVKRVGVYQIWMMNADGT
                     DAAQIIRSGQAYNDYSPVWSPRNDLIIFNQRCAKTYCNPYLLSTSPTDRSKEQGLKLP
                     FNVIFIEDIDFSPDGFYLAYEGVGDSNNFDIFFMTLSGGDKVRLTTDPANDFQPTWRP
                     VIQNP"
     gene            2520..>2700
                     /locus_tag="PKI33_09110"
     CDS             2520..>2700
                     /locus_tag="PKI33_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HNM37210.1"
                     /translation="MPNLTERQKTLLMLIIRDYIDTAQPIGSKRLAEHYHINLSSATI
                     RNEMAALTEMGYLRQP"
BASE COUNT          695 a          647 c          605 g          753 t
ORIGIN      
        1 gggttttcaa acatttcaat ttaactactc catcttcccg cccaacctcc atctgatata
       61 atgcgcgtgt ccaaatcaaa gtagtaacat ttttttaaat ctacagtctt tgctggaaat
      121 ttatatattg caggtgaatg gatttcaatg acgctggata aaggcactct catacataat
      181 cggtatcgaa tcgtagacat ccttgggcaa ggcgggatgg gctctgtata tcgcgccata
      241 gatgaaaatc ttggtgtggc tgtagcactc aaagaaaatc tgtttactac agatgagtat
      301 gctcgtcagt tccgtcttga agccgtaatc ttggcaaatt tgcgtcatcc caacttgcca
      361 cgcgtttctg accattttgt tttaggtgat ttggggcaat acttggtcat ggacttcatc
      421 gagggcgaag atcttcgcta tcgtatggaa cggttaggta tgttagagga agaagatgct
      481 gttcatattg gcgcagccat gtgtgacgcc ttggcgtatc tgcacactcg taagccgccc
      541 attcttcatc gtgatatcaa accgggcaat gtcaaaattt caccagatgg tcatatttat
      601 ctagtggatt ttgggttggc aaaggtatat caaagcgcga gccaagctac cacaacgggt
      661 gctcgtgcca tgacccctgg ctattcccct ccagaacagt atggtactgc ccgtactgac
      721 ccgcgtactg atatttactc tttgggtgca actttatacg ctgcattaac tggcgtgatt
      781 ccagaagatg gtttggcgcg tgcaatggat aatgcacaat taactccatt gcgcaagaga
      841 aattcaaagg tttcacgaaa attagctgct tctattgaaa aagcgatggc agttgaccct
      901 agcgaccgtt tccaaacggc tgaagaattc aaagctgggt tgctctcatc taagtcaatg
      961 acgcaacagg cacctggctc ttacaaggta attccacctc cgccagatgc cgttgctgct
     1021 tcatctcaca cccatgaagt aactgtggtt gacgaagctt tacctgtaga tggtaaaaaa
     1081 gaccgaaagg ctttagtagg acccgtccct gctgctgggt caaatgcaca tgaagaccag
     1141 ccttttgtct ctcccttaaa gagacaaaag gaacgagaac gtaaacgtcg tatggcttta
     1201 tggcgttttg cactttttgc aggtattttg ttgttagttc taggtagtgt tgtctttttt
     1261 gttccaggtg ttttgccggc agatgttcgt gctatggtac cttttattgg ggcaacagcg
     1321 acgccaactt ttacacttac acctgtccca acacagacgt tgccagcaac ttttacggct
     1381 acattggaac ccacctctac ccctacactg agaccgacta acacccctac tgctactttt
     1441 accccaacag cattaccagg cgaaacaatt acaattacgc cagttgcttc ctcaggaggg
     1501 acagaagtat ttatgggagg gggagcaggg caaattgctt ttgcttctga ccgaacgggt
     1561 tctcctcaga tatttttagt ggacttacgt ggtgaaagtg ccttccaagt cactaacatg
     1621 cagggtggtg cttgccaacc ggcatggtca cctgatggtc tgcgaattgc ctttatttct
     1681 ccatgtaagg gaaatgaaga catatatact aatacaagct tatatattat caatgcggat
     1741 gggaccggtt tagctacctt agatgcttct cccggcggta attttgaccc tgcttggtcg
     1801 cctgatggaa aaataattgc atatacgtct gcccgtactg gacaaatgga aattttcatg
     1861 cttccagtcg atgatccagc atctattact cagattacaa gtggtgccgc taaagttgag
     1921 tctcgcatgc cgacttggtc accagatgga tcaaagctgg tatatgtagt caaacgtgtg
     1981 ggtgtctatc aaatttggat gatgaatgct gatggaactg atgcagccca gatcatccgc
     2041 agtggacagg catacaacga ctattctccc gtctggtcgc cgcgaaatga ccttatcatc
     2101 tttaatcaac gctgtgccaa gacatactgt aatccatatt tattgagcac atcccctaca
     2161 gaccgtagca aagaacaggg gttgaaactc ccgttcaatg ttatctttat tgaggacatc
     2221 gatttctcgc cggatggttt ctaccttgcg tatgaagggg taggagactc gaataacttc
     2281 gatatcttct ttatgaccct ttctggcggg gacaaagtcc gccttacgac agaccctgcc
     2341 aacgacttcc agccgacgtg gcgaccggtt atccagaacc cataaataaa aactcccgcg
     2401 caagcgggag ttttcttatt aattcttaca ctcctcttat tgacgcctcc ataccccgct
     2461 gatattatta ttccgttata aactggcact ctgaaatttg gagtgctaaa tttttgacca
     2521 tgccaaacct caccgaacga cagaagaccc tcctgatgtt gatcattcgt gattacatcg
     2581 acaccgctca gcctatcggc tcgaagcggc tggcggagca ttaccacatc aacctgtctt
     2641 cggcgaccat tcgaaatgag atggcggcac tgactgaaat gggctacctg cgccagccgc
//
LOCUS       DAOREF010000408         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26509_length_2696_cov_3.928436, whole genome shotgun sequence.
ACCESSION   DAOREF010000408 DAOREF010000000
VERSION     DAOREF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26509_length_2696_cov_3.928436"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1598
                     /locus_tag="PKI33_09115"
     CDS             147..1598
                     /locus_tag="PKI33_09115"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNM37211.1"
                     /translation="MSHKKIKTISDPKLKLDILSKEDVQKIHEATLHIIENVGIRFPS
                     KRALRIWEEAGATVDHEKKIVKVKPYLIEDALKQAPPAYTLGARDPQQNLSLDGNHVH
                     LGTDGCGVEIIDIHTGMKRTTELQDVRDIARIADATEEVAFHWVPVSAQDAPVEARGL
                     HELKAVWENSTKHVQTESIYNVEEAKASMEMAILLAGSKEDLRKRPVLSLMQCTAPPL
                     GHDGGSLDAALIAAEHGVPTGFMTMTACMTTGPATMAGTLAVGNAEVIAATALLQLAY
                     PGAPVFYAAAQTASDPRTGVYTGGGPEDFLFGAATNVLADFYNIPLSMGSFATGAKEP
                     NWQAGLEGSLSTFMASVAMSDMLLGCGFLHGSRIWSYAEMMMDCEIFSIIEKMMHGIE
                     VNAETLALDAVAAVGPGGHYLAQKHTRNHMREIFMPQFLDRRPYSKWEETGEDARDWA
                     VNKARKVLKEHQPDPLDEKISKEFERIIKSVEK"
     gene            1708..>2696
                     /locus_tag="PKI33_09120"
     CDS             1708..>2696
                     /locus_tag="PKI33_09120"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNM37212.1"
                     /translation="MRPKLTLLSDEIIARILAEAYELLLKPGIKVQNEEARELLRQEG
                     VVIDPETNVAQIPASLVQRALESVPREFYLYDYHGNPTIHYGGDSVHFDPGSSGITML
                     NPDTLEHDTTETEHLIRLLKVAEQIPQYDAQSTAVICHDVPKDIQDSYRLYLALLYSK
                     KPIVTGAFTNKTVQEMIDMLAMFAGSAEEARQKPRAIFDVCPAPPLIWSNFGAGNLIQ
                     LARAGIPAEIVSVPLAGAAAPVTMLGTVTQHTAECLAGITIHQLAKPGSPIVWGGAAA
                     IFDMKKGATPMGAIETAMLDCSYAQVAKTLGMPTHTYLGATDSKLVDAQAGLE"
BASE COUNT          724 a          732 c          656 g          584 t
ORIGIN      
        1 gattacccgc gactgggcaa ccaaaatcaa agctgatggc acttccgaag atgcagtcgg
       61 cgcggtggca ttggcgaaac aattagttgg aaaagcataa ccaaacaatc agagagagaa
      121 gagagacata gcctcgggag ggctctatgt ctcacaaaaa aatcaagacc attagtgatc
      181 caaaactcaa attggacata ctaagcaaag aagatgtgca aaaaattcac gaagcgacgt
      241 tgcacatcat tgaaaatgtc ggcatacgtt ttccttcaaa acgagcattg cgcatctggg
      301 aagaagccgg tgcaaccgtt gaccacgaga agaaaatcgt caaggtcaaa ccgtatctga
      361 tcgaagatgc gctcaaacag gctccacctg cttatacact tggagcgcgt gacccacaac
      421 aaaatctctc actcgatggc aaccatgttc atcttggcac agacggttgt ggtgtagaga
      481 tcatcgacat tcacaccggc atgaagcgca ctaccgagtt acaagatgta cgcgacattg
      541 cccgtattgc agatgccaca gaagaagtgg catttcactg ggtgcctgtt tcggcacaag
      601 atgctcccgt tgaagcacgc ggattgcacg aactcaaagc ggtgtgggaa aactccacca
      661 agcacgtgca aaccgagtcc atttacaacg tagaagaagc caaagcctcg atggaaatgg
      721 cgattctgct agcaggcagc aaagaggatt tgcgcaagcg tcccgtcctc tcgttgatgc
      781 agtgtactgc gcctcctctc gggcacgacg gcggttcact cgacgcagcc ttgatcgccg
      841 ccgaacacgg agtgcccacc ggcttcatga ccatgaccgc ctgcatgacg accggtcccg
      901 ccacaatggc tggcacactc gccgtcggca atgcggaagt cattgccgcc accgcccttt
      961 tacaactggc atacccgggc gcgcccgtct tttacgccgc cgctcaaact gcttcagacc
     1021 cgcgaacggg cgtctacacc ggcggcggac cggaagactt tctcttcggt gcggctacca
     1081 atgttttggc agatttctac aatattccgc tttcaatggg ctccttcgca accggcgcaa
     1141 aagagccgaa ttggcaagct gggcttgagg gaagcctttc aacatttatg gcaagcgtgg
     1201 caatgtctga catgcttctt ggatgtggtt tcttacacgg cagtcgcata tggtcttatg
     1261 ccgaaatgat gatggactgt gagattttct ccatcatcga aaagatgatg cacggcattg
     1321 aagtcaatgc tgaaaccctc gcattggatg cggttgctgc ggtgggtccg ggcggtcatt
     1381 acctcgccca aaaacacact cgcaaccata tgcgtgagat cttcatgcca caattcttag
     1441 accgtcgccc ttattccaaa tgggaagaaa ccggtgaaga tgcaagagat tgggcagtta
     1501 acaaggctcg aaaagtattg aaagaacacc aacccgaccc gttggatgaa aaaataagca
     1561 aagagttcga aagaattatc aagtcggtcg aaaagtaatg cacaagaaat gagaaacgag
     1621 taacgaggtg tgagaagact catttcttgc cctcgttact cgttttatat ctctttacga
     1681 aagagaaatt ccaattagag gtgtctcatg agaccaaagc taaccctgct cagcgacgag
     1741 atcattgccc gcattttggc agaagcctac gagcttttgc tcaaacccgg catcaaagtt
     1801 caaaatgaag aagcacgcga actacttagg caagaagggg ttgtcattga ccctgaaacc
     1861 aacgtagcgc aaattccggc ttcgctcgtc caacgtgcct tggagagcgt gccgcgcgag
     1921 ttctatctct acgactatca tggcaacccc actattcact acggcggtga ctcggtccac
     1981 tttgacccgg gctcttctgg catcaccatg ctcaaccccg atacgcttga acacgacacc
     2041 accgagaccg aacacctcat ccgcttattg aaagtagcgg aacaaattcc acaatacgac
     2101 gcgcaatcta ccgccgtgat ctgtcacgac gttcccaaag atattcaaga ttcctatcgc
     2161 ctttatttgg cgttgctcta ttccaagaaa cctatcgtca ctggcgcatt cactaacaag
     2221 accgtgcaag agatgatcga tatgcttgct atgttcgcag gcagcgcaga ggaagcacgc
     2281 cagaaaccgc gcgccatttt cgatgtttgc ccagcaccgc ccttgatctg gtctaacttt
     2341 ggggcaggta acttaattca actggcacgt gcgggcatcc cagcggagat cgtctcggtt
     2401 ccgttggctg gtgcggctgc gcccgtcacc atgctcggaa cagtcaccca gcacactgca
     2461 gaatgtttgg cgggcatcac catccatcaa ttggcaaaac ctggctcccc tatcgtttgg
     2521 ggcggagcag ccgccatctt cgacatgaag aaaggcgcaa cacccatggg tgccattgaa
     2581 accgctatgc ttgattgttc ctatgcacaa gttgcaaaga ctcttggcat gcccactcat
     2641 acctatcttg gtgccacaga ctccaagctt gtcgatgcgc aagccggtct tgaaag
//
LOCUS       DAOREF010000409         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26530_length_2695_cov_3.378030, whole genome shotgun sequence.
ACCESSION   DAOREF010000409 DAOREF010000000
VERSION     DAOREF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26530_length_2695_cov_3.378030"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PKI33_09125"
     CDS             complement(<1..929)
                     /locus_tag="PKI33_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNM37213.1"
                     /translation="MTKKHTGNQIRQDFIDFFVEHGHTAVPSMSLVPGGDGTLLFTNS
                     GMVQFKDVFIGTDKKPYTRAVDSQKCMRVAGKHNDLEDVGRDDTHHTFFEMLGNWSFG
                     DYYKEEAIAWSWQLLTEVWGLPKDKLYATCFRDDKGNVPQDDEAANIWKKQPGFDPEH
                     VLFFGRKENFWQMAETGPCGPCSEIHIDLGEERDNLRGTDHVCGVNGECTRYLELWNN
                     VFIQYNLFEDGRLEPLPAKHVDTGMGFERIVSVLQGVDSNYKTDLFTGSLDVLRSLTN
                     HTEKEMLANFTPYRVICDHVRSAAFLIADGVVPG"
     gene            1368..>2695
                     /locus_tag="PKI33_09130"
     CDS             1368..>2695
                     /locus_tag="PKI33_09130"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HNM37214.1"
                     /translation="MLYKFFRWFACLAFVFAAVLSPASTVFALSFNVNSTVDEVDANP
                     GDGVCATSTGGCSLRAAIQEANATTDDDTITLPSGIYTLSIAGNNEDAAATGDLDITS
                     NMVINGTDSTNTIIDGNTLDRVFHVVNTDNFATISNVTIQNGNSPDNGGGILNAGILA
                     VNNSVVRNNFAECTGSAVYNSGGFSSMNSTIHENIGGGVFCLGMAIMNNSEASMLLNQ
                     TVISNNQPDGGLYNYGVIFLFSSTIRDNTGGGFADTPGGIYNFSGFMYINNSTISGNY
                     TSSGGGIFTGGGGEIIIANTTITNNSADSGGGIYNFPNFPSTVYMRNTILAGNTSTSN
                     EPECVGELISTGYNLIGNDTGCTFLSTTGDQVGAGSNPIDPMLGLLQNNGGFTETHAL
                     IVGSPAIDGGNPQGCQDPDGNIIETDQRGIPSPQGAACDIGAFELEEVSDV"
BASE COUNT          689 a          624 c          728 g          654 t
ORIGIN      
        1 ccagggacga cgccatcagc gatgaggaag gctgccgagc ggacatggtc gcagatgacg
       61 cgataaggcg tgaagttggc gagcatttct ttttcggtgt gattggtaag gcttcgcaac
      121 acatcgagcg aaccagtgaa aaggtcggtt ttgtaatttg agtcaacgcc ttgcaaaacg
      181 gagacgatgc gctcgaaacc cataccagta tccacgtgtt tggcgggaag tggttcgagg
      241 cgaccatctt caaaaaggtt gtattgaatg aagacgttgt tccagagttc aaggtagcgc
      301 gtgcattcgc cgttcacgcc gcagacgtgg tctgtgccgc gcaggttgtc gcgctcttcg
      361 ccgaggtcaa tgtgaatttc ggagcacggt ccacaaggac cggtctctgc catttgccag
      421 aagttttctt tgcgcccgaa gaagagcacg tgctcagggt caaagccggg ctgtttcttc
      481 cagatgttcg cggcttcgtc atcttgtggc acgttgcctt tgtcgtcacg gaagcaggtg
      541 gcgtagagtt tgtccttggg caagccccac acttcggtga gcaactgcca agaccaagca
      601 atggcttctt ctttgtaata gtcaccaaat gaccagttac cgagcatttc aaagaaggtg
      661 tgatgcgtgt cgtcacgacc cacatcttcg aggtcattgt gcttgcctgc cacgcgcata
      721 cacttctgcg agtcgacggc gcgggtgtag ggctttttgt cagtgccgat gaagacgtct
      781 ttgaactgca ccatgccgga gttggtgaag agcagcgtgc catccccacc ggggacaagc
      841 gacatcgaag gcacggcggt gtgcccgtgt tcgacgaaaa agtcaataaa gtcctggcgg
      901 atctggttgc ctgtatgttt tttggtcata ggtactccga aatttataga atagcgtgca
      961 aattatacca acgtaaggtt ccaagagcga cccgcttggc ggaatgaggt tgactcgcgt
     1021 aacaatggcg ggtcgcccct acactgaccg acaaaaaaag tcccgagaac ctgttggttt
     1081 cgggactggg tttcctttcg gttccgtctc aagccgtggc aggttcacag gtcgcgcatg
     1141 tagcggcggc ggctcgagcg gttgcggaag aaatgaggtt tggttgcatc gaatgaaatt
     1201 ataccactca gggggagggt taaccatttt aggaagagat cggggttaac tcttgtgttt
     1261 gtagaagatt ggcagttttt ggcagttaat tttccgactt tggttgtatg gtgatgttgt
     1321 tatgaagtca tgttttaggc gtatgaggca aagagaagga gatcaaaatg ttatacaaat
     1381 tctttcgttg gttcgcctgt ttggcctttg tgttcgcggc tgttctctcg cctgcaagca
     1441 ccgtttttgc gctgagtttc aacgtaaata gtactgtgga tgaggttgac gccaatccgg
     1501 gagatggagt atgtgccaca agcaccggag ggtgcagttt gcgcgcagca atacaggaag
     1561 ccaatgctac aaccgatgat gacacgatca ctcttccatc tgggatctat acattgagta
     1621 ttgctggtaa caatgaagac gccgccgcca caggggacct tgatatcaca agcaacatgg
     1681 tcatcaatgg tacagacagc acaaatacca tcattgatgg gaatacattg gatagagtgt
     1741 ttcatgttgt aaacacggac aatttcgcaa ccatctccaa tgtaaccata caaaacggaa
     1801 attctccaga caacggtggt ggaatattga atgctggaat attggcagtg aacaacagcg
     1861 tagttcgcaa taacttcgcg gaatgcacag gcagcgccgt ttacaacagc ggtgggtttt
     1921 catcaatgaa cagcaccatt catgaaaaca tcggcggcgg cgtcttctgt ctgggaatgg
     1981 caataatgaa taactcagaa gcgtccatgc tgctaaacca aacagtgata agcaacaacc
     2041 aaccggacgg cggcctgtac aattatggtg tgatattcct gtttagcagc acgatccgcg
     2101 ataatacagg cggaggattc gcagacacac ccggcggtat atataacttt tcgggtttca
     2161 tgtatatcaa caatagcaca attagcggta actacacttc ctctggcggc ggcatattta
     2221 cgggcggagg cggggagatc atcatagcaa acacgacaat taccaataac tcagctgatt
     2281 ccggcggagg catttacaat tttccaaact tcccaagcac tgtatacatg cgaaatacca
     2341 tcctggcagg caatacgagt accagcaacg aaccggaatg tgtcggcgaa ctaatctcca
     2401 cagggtacaa cttgatcgga aatgataccg gctgtacctt cttaagcact acaggtgatc
     2461 aggtgggggc gggctcaaat ccaattgacc ctatgcttgg tcttctgcaa aacaacggcg
     2521 gctttactga aactcatgcc ctcatcgttg gaagccccgc cattgatggc ggtaatccac
     2581 aaggttgcca agacccggat ggaaatataa tagaaacaga ccagcgtggc attccaagtc
     2641 ctcaaggcgc agcctgtgat attggtgcgt tcgaacttga agaagtttcc gatgt
//
LOCUS       DAOREF010000410         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26628_length_2689_cov_5.100607, whole genome shotgun sequence.
ACCESSION   DAOREF010000410 DAOREF010000000
VERSION     DAOREF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26628_length_2689_cov_5.100607"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1809
                     /locus_tag="PKI33_09135"
     CDS             145..1809
                     /locus_tag="PKI33_09135"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM37215.1"
                     /translation="MTPNIFMSYSRRETGFVDDLTHRLEKAGFNVWLDYRRLIPGTPW
                     AGQIQKGLQEAEVVLLVVSKDSIASQYVELEWRHVLNEKNKRIILVIFEAVTLPDELK
                     KYEWVDFRGSYEAGIKELISQLNQPIQETQAPPESGFKAPFIVWVSILLSAIAGVYSL
                     LSFWSVLLPLILVPLAWQVYKRNYNFSRVQTALWMSPLLNLLGLLLFSELGYLNAEGE
                     NFAVYYFRINPLLGVLVNILQWLPYSAFVLIFLFRSASMQRWGKPEANMPKFANPYRP
                     KNPNPRPVKFFIDHAEQDSVIAKDISNKLIQYGHLQEADIKSAESVLVLISRFNTDTS
                     ADPEHQIVFPVLVQTAQPSEKLSHVQWIDYRKGLKNLDALAQLLPDPTLLLTALGTRP
                     TNGTQIVMPNIFSRVVNFLTFILLLNVSTFFIYILELWANGLGYIQQYEPAFIFSNLA
                     QTALAVLVACGLGFFIIKSVTERRGLFASIPPFFAGLFGLFLILMYQYTLADYMTTLF
                     EDYGETEIYGFITIPYLFLIFGGIVLLIMIFFNWKELRRWFPKKGK"
     gene            1828..>2689
                     /locus_tag="PKI33_09140"
     CDS             1828..>2689
                     /locus_tag="PKI33_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM37216.1"
                     /translation="MMPNTFISYSRQCIGFVDDLAHKLQKQGFSIWTDYLSLVPGRPW
                     AEQIEKGLQEAELLLLVVSPESVSSRSVEIEWRHFLEHKKRIILLIFQAVPLPPELAG
                     LEWVDFRGAFAPAMKELLKRIEAPSPQRGLAPQSGFKAPFVVWVAAGLSVINAVYSLF
                     AFWTLLIPWVLVPLPARIFKREFNLSQVQTALWTQPIALFFTLGLFLELKLINIDVDF
                     DYSNYFSLYNMVLMSLFIQIYLIPFFSILLLIVLRLPGMQRWGRPEASAPKFANLYRP
                     NNQTLILIVTI"
BASE COUNT          675 a          631 c          585 g          798 t
ORIGIN      
        1 aaagtagaag tcatacaggt agtatattgg gattttgaaa attggtaaat cgggaattgt
       61 cagtttggtg ttttataatg acaaggttta ctggacaatt aagcatgttc gcctgcaacg
      121 catctcaatt tattggagac tttcatgaca ccaaatatct ttatgagtta ttcaaggcgc
      181 gaaacagggt ttgtagatga cctaacccat aggctggaga aagccggttt taacgtctgg
      241 ctcgattacc gaaggttaat tccaggcacg ccgtgggctg ggcaaattca aaaaggcttg
      301 caagaagcag aagtggtttt gttggttgtt tccaaagatt caattgcttc acagtatgta
      361 gaattagagt ggcgacatgt attaaacgaa aagaacaagc gcattattct tgtcatcttt
      421 gaagccgtga cgttaccgga tgaattgaaa aaatatgaat gggtggattt tcgaggtagt
      481 tatgaagctg gcatcaaaga attaatttca caactcaatc aacctattca agaaacacaa
      541 gccccgcctg aaagcggatt caaagcgccc ttcatcgtgt gggtaagcat ccttttgagc
      601 gccatcgctg gcgtttattc gctgctttcc ttttggagcg tattgcttcc attgattctg
      661 gttccgctcg cctggcaagt ctataaacgc aactataact tctcacgcgt ccaaactgcc
      721 ttgtggatgt cacctttatt aaacctgctt ggcttactac tctttagtga acttggttac
      781 cttaatgcgg aaggcgaaaa ttttgcagta tattattttc gcatcaaccc attattgggg
      841 gtattggtaa acatcttaca gtggctgccc tattcggctt tcgttttgat ctttcttttt
      901 cgttcggcaa gtatgcaacg ttggggcaag cccgaggcga acatgccgaa atttgccaat
      961 ccatatcgtc cgaaaaatcc caatccccgc cctgttaaat tctttatcga tcatgcagaa
     1021 caggattctg ttatcgcgaa ggatatatca aacaagttga tccagtatgg gcatcttcaa
     1081 gaagcggaca tcaaatctgc tgagtcagtg ttggtattaa tctcgcgctt caacaccgac
     1141 acgagcgctg accccgaaca tcaaatcgtt ttccccgttt tggttcagac cgcacaacct
     1201 tcagaaaaac tttcgcatgt gcaatggatt gattatcgca aagggttgaa aaatttagac
     1261 gcattggcac aattattacc agacccaacc ctattattaa ctgcattggg cacgcgtccc
     1321 accaatggca cacaaattgt gatgccaaac atcttcagta gagttgtcaa cttcttaact
     1381 tttattcttt tgctaaatgt cagtacgttt tttatctata ttctggagct atgggcaaat
     1441 gggttgggat acatccaaca gtatgaaccg gctttcatct ttagcaacct ggcgcaaacg
     1501 gcattggctg tgcttgtggc gtgcggactt ggatttttca taatcaaatc ggtaacagaa
     1561 aggcgcggac tctttgcatc catcccgccg ttttttgcag ggctatttgg tctgtttctc
     1621 attctcatgt atcaatacac cctggcagat tacatgacca cactcttcga agactatggc
     1681 gaaacagaaa tttatggttt tattaccatt ccatatttgt ttcttatttt tggcggaatt
     1741 gttttgctga ttatgatctt cttcaattgg aaagagttac gccgttggtt ccctaaaaaa
     1801 gggaaataaa tttttaacct ctaccctatg atgcccaaca cctttatcag ttattcacgg
     1861 caatgcattg gttttgtaga tgaccttgcc cacaagttgc aaaaacaagg cttcagcatc
     1921 tggacagatt acctcagcct ggtgccgggg cgaccatggg cagaacagat cgaaaaggga
     1981 cttcaagaag ccgagctgct gctcttggtc gtctcaccag agtctgtttc ctctcgcagc
     2041 gtggagatcg aatggcggca ctttctcgag cataaaaagc gcatcatctt attgatcttt
     2101 caagccgtgc ctttgccgcc agagctagcc ggcttggaat gggtggactt tcgcggtgcc
     2161 tttgccccag ccatgaaaga actcctgaaa cggatcgagg cgcccagccc acagcgtgga
     2221 ctcgctccgc agtctgggtt caaagccccg ttcgtcgttt gggtggcggc gggtctcagc
     2281 gtcatcaatg ccgtttattc cctcttcgct ttttggactc tgttgattcc ctgggtgtta
     2341 gttcctctgc ccgcgcgcat cttcaaacgc gagttcaatt tgtcgcaagt ccaaaccgcg
     2401 ttgtggacgc aacccattgc tttgtttttc acactgggct tgtttctcga attgaagctc
     2461 atcaacatcg atgtagattt tgactacagc aattacttct cgctctataa catggtgttg
     2521 atgagtctct tcatacagat ctatctcatc cccttctttt ccatcttgct attgatcgtc
     2581 ttacgcttac ctggcatgca acgttggggc agacccgaag ccagtgcacc caagttcgcc
     2641 aatctttatc gcccgaacaa ccagaccctc atcctcatcg tgaccatcg
//
LOCUS       DAOREF010000411         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26789_length_2679_cov_2.918445, whole genome shotgun sequence.
ACCESSION   DAOREF010000411 DAOREF010000000
VERSION     DAOREF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26789_length_2679_cov_2.918445"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKI33_09145"
     CDS             <1..508
                     /locus_tag="PKI33_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37217.1"
                     /translation="ALKFEQGIKTQVSHETHRGRILFNPWVTQEVLGEFPELNLCCDF
                     SHWVCVSERLLLDGEEEYIKAAAEHCLHIHARVGYEEGPQVPDPRAPEYQTHLDAHEH
                     WWQIVWEAQSRRGFTATTLTAEYGPPLYLHTMPHTNQPAANLWDVCEWQAQHTAEHYA
                     NWKAKLVK"
     gene            537..1289
                     /locus_tag="PKI33_09150"
     CDS             537..1289
                     /locus_tag="PKI33_09150"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF004079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNM37218.1"
                     /translation="MKIGIIGALDEELIPIRALSSHLEKIERGNRTYWHGDMHGHQVY
                     LTRCDPGKVNAVIATQQLLDFFEPDVLFNMGSSGALAPNLEVGDLVIATDAIQHDFDL
                     TGWGMKPGEILFDVVTKPETGQLGFTSRHAFATNPKLTELAFQSAQSVSLDEIGGHAP
                     KVYKGRVVSGDQFISSVEKAGQLLTIHNALAVDMEAAAIAHTCEINKVPFLCVRAMSD
                     KADHSANVSFTDFLVAATDNYGKVFDKLIKQL"
     gene            1406..1852
                     /locus_tag="PKI33_09155"
     CDS             1406..1852
                     /locus_tag="PKI33_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37219.1"
                     /translation="MSKRLPGILVRGHQVASRPSAAYPYSSLEKQKPYFKALGLDLSS
                     YFNGTLNISIAPFVFEMTAPEFTFPLVEWTDLHPPETFSFSRCKVIFKNADYAGWVYY
                     PHPETKKTHFQNPSLVEVISYQIPEIQYGDTLEVELNTDEVTVRAG"
     gene            1965..>2679
                     /locus_tag="PKI33_09160"
     CDS             1965..>2679
                     /locus_tag="PKI33_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNM37220.1"
                     /translation="MRIGFISDIHGNFTALEAVLADIKKQNIDQLICLGDTVSLGPQP
                     REVLDALREWNAVTIMGNHDQATLEPERCTEFEITEHLIPDLYWGREQLSKEDLQYLT
                     SFQMTKNVQLERGIELLAYHGSPKGTTHLVLATTPAETLNEYYGGETASIFIGGHSHI
                     QMERRHGSKLFLNAGSVGNAFQFAYTPGNPPNLLPWAEYAIVEQEGNFLHADMRRIYF
                     DIDALLARVKESKLPGANWW"
BASE COUNT          736 a          700 c          605 g          638 t
ORIGIN      
        1 agcgctgaaa ttcgagcaag gaataaaaac tcaggtatca catgaaaccc accgcggacg
       61 aatcttattc aacccgtggg tcactcaaga agtcttgggc gaattcccag agctaaacct
      121 gtgctgtgac ttcagccatt gggtttgtgt gagcgaaagg ttattgctcg acggtgaaga
      181 agaatacatc aaagccgcgg cggaacattg tctgcatatc catgcacggg ttgggtatga
      241 agaaggaccg caagttcccg atccgcgtgc gccagagtat caaactcact tggatgcgca
      301 cgaacattgg tggcaaatag tctgggaggc gcaatctcgt cgcgggttca ccgccacaac
      361 cctcaccgcc gaatacggac cacctcttta tttgcacacc atgccgcata caaatcaacc
      421 tgcagcgaat ctatgggacg tttgcgaatg gcaagcccaa cataccgccg aacattatgc
      481 aaactggaaa gcaaagctag taaaataatc aaaaccaact caaaaggata cgtcccatga
      541 aaatcggcat catcggcgca ttagacgaag aactcatccc aatccgcgca ctttctagcc
      601 atcttgaaaa gatcgaacgc ggcaaccgca cctactggca cggcgatatg cacggacatc
      661 aggtctatct cacccgctgt gacccaggca aggtcaacgc agtgatcgct actcagcaac
      721 tgctcgattt ctttgaaccg gatgtactct tcaacatggg cagttccggc gcgctcgccc
      781 caaaccttga agtcggcgac cttgtcattg ctaccgacgc cattcaacat gacttcgacc
      841 tgaccggctg gggtatgaaa cccggcgaaa tcctttttga tgtcgtcaca aaaccagaga
      901 cggggcaact cgggttcacg tctcgccacg cttttgcgac caatcccaaa cttaccgagt
      961 tggcatttca atccgcccaa tccgtttcac tggatgaaat tggtggacac gccccaaaag
     1021 tctacaaagg tcgcgttgtc agcggcgacc agttcatcag cagtgttgaa aaagccggtc
     1081 aactcctcac catccacaac gccctcgcgg tagatatgga agccgccgcc atcgcgcaca
     1141 cctgcgagat caacaaagta cctttcctgt gcgtccgtgc catgtccgac aaagccgatc
     1201 actccgccaa tgtcagcttc accgacttcc tcgtcgccgc caccgataac tacggcaaag
     1261 tctttgacaa attgataaaa caactttaat accaagaggt gaagaggatg attgcttcgc
     1321 ctcttttctt tgtccatacc tgattgccca caatataaag tttctacact gttgcttttt
     1381 cttccacgcc tgatatactg gcttcatgag caaacgcctg cccggcatcc tcgtccgcgg
     1441 acatcaagtt gcatcgcgcc cgtcggcagc gtacccatat agttcgcttg aaaaacaaaa
     1501 accttatttc aaagcgctag ggctggattt atcctcgtac ttcaacggca cattgaacat
     1561 ttccatagcg ccgtttgttt tcgaaatgac cgcgcccgaa tttacctttc cgctcgtcga
     1621 gtggacagac cttcacccgc cagaaacttt ttccttctcg cgttgcaaag tcatctttaa
     1681 aaatgcggat tacgcaggtt gggtgtatta tccgcaccca gaaacaaaaa agacccactt
     1741 tcaaaatcca tctctggtgg aagtaatttc ttatcaaatt ccagaaattc aatatggcga
     1801 tacactggaa gtggaattaa acacagacga ggtcacggtt cgggctggct gaatgtttga
     1861 gagggcaaac aggcagaagt ctggggtgaa ccggggtttt atatcaccca aaagttctat
     1921 tttgggaata aatccatata ccgtcgtgta caattaggta gattatgcgt attggattta
     1981 tttcagatat tcacggcaat tttacagcgc tcgaagccgt ccttgcagac atcaaaaaac
     2041 agaacatcga ccaattaatt tgcctgggcg atactgttag tttgggcccg caacccagag
     2101 aagtgctgga tgcattacgt gagtggaatg ccgtcactat catgggcaat catgatcagg
     2161 cgacattgga accagagcgc tgtacagagt tcgagatcac agaacatttg ataccagatc
     2221 tgtattgggg acgtgagcaa ctttccaaag aagatttgca atatctaact tcatttcaaa
     2281 tgacaaaaaa cgttcaactc gaacgcggta ttgagttgct cgcttatcat ggttctccca
     2341 aagggacgac ccatttggtt ctggcaacaa cccctgcaga aaccttgaat gagtattacg
     2401 gcggggaaac cgcttcgatc tttattggtg gacattcgca cattcaaatg gaacgccgcc
     2461 atggcagtaa attatttttg aatgcaggca gtgtgggaaa tgcatttcaa ttcgcataca
     2521 ccccgggcaa cccgcccaat cttttacctt gggcagaata tgctattgtg gagcaagaag
     2581 gaaatttctt gcacgccgat atgcgccgta tctattttga catcgatgcc ttattggcgc
     2641 gcgtaaaaga aagcaaatta ccgggcgcga attggtggt
//
LOCUS       DAOREF010000412         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26838_length_2676_cov_5.636780, whole genome shotgun sequence.
ACCESSION   DAOREF010000412 DAOREF010000000
VERSION     DAOREF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26838_length_2676_cov_5.636780"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKI33_09165"
     CDS             complement(<1..226)
                     /locus_tag="PKI33_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37221.1"
                     /translation="MTEIKVKIMGMNQFPIKFRRIAVFAGIFILVLFVIEFNSRLEEL
                     NRLNEQRDEMRAQATQAVQTQIALQTQVAYA"
     gene            318..851
                     /gene="rplI"
                     /locus_tag="PKI33_09170"
     CDS             318..851
                     /gene="rplI"
                     /locus_tag="PKI33_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNM37222.1"
                     /translation="MKVLLIKDVYKLGRAGDIKKVADGYGRNFLLPQGLAVLASEGAM
                     KQVQKIKAQAEIRRSTQNNELKGVADLIKDVTLIFPAKAGDTGKLYGSITTGDIATAL
                     SEKIRFEVKRQQVDTQPIRTLGEFTAHVRLTMDLVPDIKIIVHREGESPESAAGQHEE
                     PAKAEKKEEAAAETKAE"
     gene            909..2597
                     /locus_tag="PKI33_09175"
     CDS             909..2597
                     /locus_tag="PKI33_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNM37223.1"
                     /translation="MQETIGQRLKREREARYLSLEKAAEETRIRRVFLQALEADDYSV
                     MPSAAQGRGFLRNYAEYLNINIDELIAEIQRNPIPLAEVSGPLPQVNLAETELPPLTE
                     AEDEKSAPPFWTRFLNRKPKEESTPEAPQEPSPEIEQILEEQPPAVVEEEKPKTRGRK
                     KKIVVEEAIVEPVAELKVEEPESQPSVESQPDGQEQVEIQEEPKQAWWGRVVSLFKIK
                     VQKQDSAFEVVLSEDKEDIPQNGVSSIPDKPADVIMAEIGKMLRERRELISLTVDEVE
                     RHTHLRAVFVKALEEGAFDKLPSTVQTRGMLSNYASFLDLDVDTVLLRYADALQARRR
                     EKYAETPREKIQMQVNPSMPVLRTFIAGDLLFGVIMIAVIVALAIWGVGRLVSSDQQT
                     QVQPTSPSIVDVLGNLSDATASPAVTFVAVDDPAIANAGTAAEGTVTPAGSEVASTAN
                     VVVNVYALERVFVRISVDGEVAFEGRLAPRESRFFEANDQVVILTGNGSGLRITYNGE
                     DLGLMGGPGEVVNRVYLITGVVTPTATIPPTPTNTPLTTPTLTPTVTPTGTATP"
BASE COUNT          726 a          649 c          654 g          647 t
ORIGIN      
        1 cggcgtaagc aacctgagtt tgtaacgcaa tctgggtttg gacagcttgc gtagcttggg
       61 ctcgcatctc atcacgctgt tcgtttaaac gattgagttc ttcgaggcgg gagttaaatt
      121 caatcacaaa aaggacaaga ataaaaatgc ctgcaaagac tgcaattcgc ctaaacttga
      181 tagggaactg gttcatgccc attatcttaa ccttaatctc tgtcattggc aaaccattca
      241 attgtggtaa aatcttgccc cgcgggtcgt tgtacccttc gacctgactt caaattttgc
      301 cagaaagggc ttaaatcatg aaagtattac ttatcaaaga cgtttacaaa ctcggtcgcg
      361 ccggcgacat caagaaagtt gccgatggtt acggacgcaa cttccttctc cctcagggtc
      421 tcgctgttct cgcttctgaa ggagcgatga agcaggttca gaaaatcaag gcgcaggctg
      481 agattcgccg ctctacccag aacaacgaac ttaagggcgt tgctgacctc atcaaagatg
      541 taactcttat cttccccgcc aaggctggtg ataccggaaa gctttacggt tccatcacca
      601 ccggtgatat cgccactgca cttagcgaaa agatccgctt tgaagtcaag cgccaacagg
      661 tagataccca acccatccgc acgctcggcg aattcactgc ccacgttcgc ctcaccatgg
      721 acttggttcc tgatatcaag atcatcgtcc accgcgaagg tgaaagcccc gaatcagccg
      781 ctggtcaaca cgaagaacct gccaaggctg agaaaaaaga agaagccgca gcagaaacca
      841 aagcggaata acaaaaatgt caaaacatca ccggcgcaag tcggtgatgt ttataataca
      901 atacttccat gcaagaaacc atcggtcagc gattgaagcg cgaacgcgaa gcacgttatc
      961 tctcgcttga aaaagcggca gaagaaacac gtattcggcg cgtcttcctc caagctctcg
     1021 aagcagatga ttattccgtc atgccgtctg ccgcgcaggg gcgtggtttt ttgcgcaact
     1081 atgccgagta tctcaatatc aatattgatg aactcatcgc tgaaattcaa cgcaacccca
     1141 ttccactcgc agaagttagt ggaccacttc ctcaagtcaa tttagcagag acggaacttc
     1201 ctccattaac agaagcagag gatgaaaaat ccgcgcctcc tttctggacc cgcttcctga
     1261 atcgcaaacc caaagaagag tctacgccag aagcgcctca agaaccctct cccgaaattg
     1321 aacagattct ggaagagcaa cctccagcag tagttgaaga agaaaaaccg aaaacacgcg
     1381 gcaggaagaa aaaaattgtc gttgaagaag caatagttga accagttgct gaattaaaag
     1441 tggaagaacc cgaatcccag ccatctgtgg agagtcagcc agatggacag gaacaggtcg
     1501 aaatccagga agagccgaag caagcttggt gggggagagt tgtttcgtta ttcaagataa
     1561 aagttcaaaa gcaagactcg gcgttcgagg ttgtgctttc agaagataag gaagatatac
     1621 cgcagaacgg tgtttcgtcc attcctgata agccggctga tgtcattatg gcagagatcg
     1681 gtaagatgct gcgcgaacga cgtgaactca tcagtttgac tgtcgatgaa gtggagcgtc
     1741 atacgcattt gcgtgcggtg tttgtgaaag ccttggaaga aggtgctttt gataaactgc
     1801 cttccaccgt gcagactcgt ggcatgcttt caaactatgc cagttttctt gatctggatg
     1861 tagacactgt ccttttgcgt tatgccgatg ccttgcaagc aaggcgccgt gaaaaatatg
     1921 cagagacccc gcgcgaaaaa atccaaatgc aggtcaaccc ctctatgccg gtgttgagaa
     1981 cttttattgc gggcgatttg ctttttggtg taataatgat cgcggtcatt gttgctttgg
     2041 caatttgggg cgttggaagg ctggttagct cagaccagca aacacaagtt cagccaacgt
     2101 ctccatctat tgtggatgtg ttgggcaatt tgtctgacgc gacggcttca cctgcagtga
     2161 cctttgttgc ggtagatgac cctgccattg ccaatgctgg cacagccgca gaaggcacag
     2221 ttactccggc gggttctgag gttgcttcga ctgcaaatgt agttgtgaac gtatacgcat
     2281 tggagcgcgt gttcgttcgt atttctgtag atggtgaggt tgcctttgag ggacgtttgg
     2341 caccgcgcga aagccgtttc tttgaggcaa acgaccaggt tgtcattctg actgggaatg
     2401 gttctgggct gagaatcaca tataatggtg aagatcttgg tctgatgggc ggacccggcg
     2461 aagtggtgaa ccgggtttat ctcattacag gtgttgtcac gcctacggcg accatcccgc
     2521 cgacacccac gaatacgccg cttacaaccc caaccttaac gccgactgtc actccaacag
     2581 gaacggcaac tccgtaattc ttagtttcat caatttattg tcaggatcaa acgtgtctaa
     2641 aaatactttt catttagttt ctctcggttg ttcaaa
//
LOCUS       DAOREF010000413         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26881_length_2674_cov_6.545246, whole genome shotgun sequence.
ACCESSION   DAOREF010000413 DAOREF010000000
VERSION     DAOREF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26881_length_2674_cov_6.545246"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKI33_09180"
     CDS             <1..581
                     /locus_tag="PKI33_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37224.1"
                     /translation="IVSRTGYTGEKMAFELFVHPERAVDFWNGVMKAGEPFGVKPIGL
                     GARDSLRTEAGLPLYGHEMGLGSGKFDGRDLGVAEGGFGSYVKTYKPWFIGRDAFVAR
                     EKERKSVVVRFTFDEQRTRMAHNGDPVVNANGERIGFVTSCAIDNARFITGQAFVDLA
                     YAKEGTVIGVNQGGNTDRPAGLAKVVSRFAKL"
     gene            686..1321
                     /locus_tag="PKI33_09185"
     CDS             686..1321
                     /locus_tag="PKI33_09185"
                     /inference="COORDINATES: protein motif:HMM:NF022440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="HNM37225.1"
                     /translation="MSDNNLPLLDIGKLSEPATKLIQVVADAVGVIYEPTRIKRKAKA
                     DAEAEIIKTKAKLQSDTLKLRAKKRKQTNELKQQKNIESIIEKSIPQLPESVSSQPVD
                     DDWVVQFFNFAQDVGNDEMQVIWARLLAGEVANPGSYSLRALHAIRMLNPTDAIYFAK
                     FCNYLWDRKEYFFTEETQKHLDKHGLSDYVIFHLKTLGLITTELDFPLKGA"
     gene            complement(1855..2202)
                     /locus_tag="PKI33_09190"
     CDS             complement(1855..2202)
                     /locus_tag="PKI33_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37226.1"
                     /translation="MQSEKSNFTNIERLIAVHGNRFILHRILHALPLDKFDMIDLDMS
                     PIYKQAAQETLSELEGLHDVIRLHFPNEYLNTLFKSRSKCEQLVSFLPKSLPIPKTKY
                     FSKSEDLQPPLFN"
     gene            complement(2305..2604)
                     /locus_tag="PKI33_09195"
     CDS             complement(2305..2604)
                     /locus_tag="PKI33_09195"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HNM37227.1"
                     /translation="MVRFISLENCPPDFAVEVTTATNTQNKIELRDFASLDPTQERLR
                     QDLVLDLQKIYAYKSGEVISSPQDGCTIDEATIALACSLPEVQFAADAKRYISQL"
BASE COUNT          788 a          499 c          614 g          773 t
ORIGIN      
        1 tgatcgtttc gcgcacgggt tataccggcg agaagatggc gttcgagttg ttcgttcacc
       61 ccgagcgcgc cgtggacttt tggaacggcg tcatgaaagc gggtgaaccc ttcggcgtga
      121 agccgatcgg tttgggtgcg cgtgactcac tgcgcaccga agcgggcttg ccgttatatg
      181 gtcatgagat gggcttgggt tccggtaaat ttgacggacg cgatctgggc gttgccgaag
      241 gcggatttgg ttcgtatgtg aagacctaca agccgtggtt catcggacgc gatgcctttg
      301 tggcgcgtga gaaggaacgc aagagtgtgg tggtgcgttt caccttcgac gaacagcgca
      361 cgcgcatggc gcataacggt gacccggtgg tcaatgccaa cggcgaacgc atcggctttg
      421 tgacctcctg cgccattgac aacgcgcgct tcattaccgg ccaggccttc gtggacctgg
      481 cttatgccaa ggaaggcacg gtcatcggag tcaatcaggg cggcaatacg gaccgccccg
      541 ctgggttggc gaaggtggtg agtcgcttcg cgaagttgta ggagaagaaa gatgggacgg
      601 cgtcagggcg ggacgccgtc ccattgtgat gaagaagaga ttatctaatt gttaggatag
      661 caatctcata gaaagggtgt ttattatgag tgacaacaat ttgccattac ttgatattgg
      721 aaaacttagt gagcctgcaa ctaaactaat acaggttgtt gctgatgctg ttggagtaat
      781 ttacgagcct actaggataa agaggaaagc taaagctgac gcagaagcag agataattaa
      841 gacaaaggca aaattacaat ctgacacctt gaaattgcga gctaaaaaaa gaaaacaaac
      901 caatgaattg aagcagcaaa aaaatatcga gtcaattatc gagaaatcta ttccacaatt
      961 gccagagagt gtttctagcc aaccagttga tgatgattgg gtcgttcagt ttttcaactt
     1021 tgctcaagat gtcggcaatg acgaaatgca agtaatttgg gctcggctat tggcaggtga
     1081 agtagctaat ccaggatcat attcacttcg cgcactacat gcaattcgga tgttgaatcc
     1141 aactgatgca atttatttcg ccaagttctg taattattta tgggatagaa aagaatattt
     1201 ctttacagaa gagacacaga aacatcttga caaacatggc ctcagtgact atgtcatctt
     1261 tcacttaaaa acgttggggc ttataactac tgaattagat ttccctctca aaggagctta
     1321 aactatcgca ctaaattatt tcgacagaaa atatgcaata agcaataaaa gtttcagtga
     1381 tcataactta agagttttta tattaactga attaggaaaa gaaattgcaa ctttatgtga
     1441 tgtaaaacct gattatgaat attgtgaaat agtggtgtcg aaatgggaca ttgaaaatat
     1501 atatgtagag agaatttagg ataattgcta gtttcttgaa tagtactggt ccttacccta
     1561 atttacatca taatcaagtt tctctgattg tttcccttta agagagagtg ttttttgttg
     1621 ctgctctttg aaacaatcac caaaagtata gttaaagagc aattatcatg tataaaaata
     1681 aaagaaaatc aaaccgcctt tgaggactaa attttctgcg tattcggttc tggataggcg
     1741 atacagaacc ttccccatgc cataatgttg cagcaagtca ttgaacgact tacggctttt
     1801 tcgggctttg ttggcaatct gcgcatgaat tcccatcttt tttgatcttc taaactaatt
     1861 aaagagggga ggctgtaaat cttcgctctt tgaaaaatat tttgtcttag gaatgggaag
     1921 gcttttgggc aggaaactaa caagttgctc acattttgaa cggctcttaa ataatgtatt
     1981 taaatattca tttgggaaat gcaatctaat tacatcatgc aacccttcta gttctgatag
     2041 agtttcttgt gcagcttgtt tatatattgg tgacatatct aaatctatca tgtcaaattt
     2101 atcgagaggt aaagcatgaa gaattctatg taaaataaat ctgttgccat gcactgcaat
     2161 taatcgctca atattagtga aattactctt ttcggattgc aaagtgcttt caacaattct
     2221 agaaatctca acagagcgcc ataaacgata tgcagagagg ttttcgttga aaattaacgt
     2281 ataagggggt tcgtgtatat ctccctaaag ttgactaata tatctctttg catcagcagc
     2341 aaactgtact tcaggcaatg aacaagcaag cgcaatcgtt gcttcatcaa tagtacatcc
     2401 gtcttgtggc gatgaaatta cttcaccgct tttgtatgcg tatatttttt gaaggtctaa
     2461 cacaagatct tgtcttaatc tttcctgggt tggatcaaga gatgcaaaat ctcgaagttc
     2521 aattttattt tgagtatttg ttgctgttgt aacttcaacc gcaaaatcgg gcgggcaatt
     2581 ttccaaagaa atgaagcgca ccaaaacacg cccatttcct gacctgcttt agttattgtg
     2641 tccaactgga atgagagtca gagcaggttg gatt
//
LOCUS       DAOREF010000414         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26886_length_2674_cov_4.093929, whole genome shotgun sequence.
ACCESSION   DAOREF010000414 DAOREF010000000
VERSION     DAOREF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26886_length_2674_cov_4.093929"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1575)
                     /locus_tag="PKI33_09200"
     CDS             complement(226..1575)
                     /locus_tag="PKI33_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNM37228.1"
                     /translation="MAWEPKIMRKVRIRNSQKKGVLGKLLTAVAEAGGNVGSINLLTE
                     TSRHVVRDITITAEDEKSLAAVLAAMETNEGTKILEVRDEVLELHQKGKIAIRSRYPI
                     ESLTTLRRVYTPGVAEVCNRIAKDPSQARLYTSISHMVAIVTDGTAVLGLGDIGPLAG
                     MPVMEGKAMLMETLVGLSGIPILLNTKDTDQIVNTVAAIAPTFAAIQLEDISAPRCFE
                     IEERLQAMLDIPVLHDDQHGTAVVSTAALMTATREAGMDMKKAVIGQIGLGAAGQAIG
                     QMMMRLTGNPVHGADLSPEALNRFEKLGGIKSNLKDIMANCDIVVATTGAPNLIKPEM
                     VRKGQIILALSNPNAEIDPEVAMERGAAFAADGKSVNNVLGFPGIFRGAVDSNAPRIT
                     QEMLLAAAETIASMTPSGELVPNPLDKKVHHAVARAVAETAMKQGIARADYVPYVEE"
     gene            complement(1669..2385)
                     /locus_tag="PKI33_09205"
     CDS             complement(1669..2385)
                     /locus_tag="PKI33_09205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNM37229.1"
                     /translation="MPNKNIKAVLFDLDGTLRLNQPASRDVFAECVQGMGLVPSEEDQ
                     TRAERWEHLYFASSLEIQEDSKIFKDDRKAFWVNFTKRRLVALGVHKPQAEELASHVS
                     DCMGMNYKPQPYLPEGIHELLKHLQRTGYVLGVVSNREEPFHSELEELGLKEYFHFSL
                     TGGEVQSFKPHPRIFERALELSGTVASETMYVGDNYFADVVGALRSGLLPVLFDPTGL
                     FPDAECDVIRSYAEFYKLLM"
BASE COUNT          663 a          705 c          589 g          717 t
ORIGIN      
        1 tttagagccg aagaagagca tgggaatcaa cgccgcaatg gctccgccca agtgaatgat
       61 aaagacgctt tccaacggtc ctagcttttg tgtgatcatg ctcgaaagtg gaccttgcag
      121 tcctatcgcc atcccgcccg cgagaccgat caaaataatg atgatgagag tatccattta
      181 atccttatac atcgcaaaca gcagtctgcg gtctattgtc caagtttatt cttccacata
      241 cggcacataa tccgcacgag caatgccctg cttcattgcc gtctctgcca cggcacgcgc
      301 cacagcatga tgaacctttt tatccaacgg gttaggcacc aattccccag acggcgtcat
      361 actggcaatg gtctctgccg ctgcaagcaa catctcttgt gtaatacgcg gcgcattcga
      421 gtccactgcc ccgcggaaga tgccgggaaa gcccaaaaca ttgttcacag attttccatc
      481 cgctgcaaat gccgcacccc gctccatcgc cacttcaggg tcgatctcag cattcgggtt
      541 cgaaagcgcg aggataatct gtcccttacg caccatctcc ggcttgatca ggtttggcgc
      601 acctgtagtt gccacaacaa tatcgcagtt cgccataatg tctttcaaat tggatttaat
      661 gccgcccaac ttttcaaaac gattcaacgc ctctggactc aagtccgcgc catgaacggg
      721 attgcccgtc aaacgcatca tcatctgacc gatcgcctgc ccagccgccc caagcccgat
      781 ctgcccgatg acggctttct tcatatccat gccggcttcg cgagttgccg tcatcaatgc
      841 cgcggtagaa accaccgccg tcccatgttg gtcatcatgc aatacaggga tatccaacat
      901 ggcttgcaag cgttcttcaa tttcaaaaca acgcggagcc gaaatatcct ccaattgaat
      961 cgccgcaaag gttggggcaa ttgccgctac ggtattcaca atctggtctg tatcttttgt
     1021 attcaacaaa atcggaatgc ccgaaagccc aaccaacgtt tccatgagca tggctttgcc
     1081 ttccattacc ggcatccccg ccagtggacc gatatccccc agccctagca cggctgtccc
     1141 atcggtaaca atcgccacca tgtggctaat gctcgtatac agccgagctt gcgatgggtc
     1201 tttggcaatt cgattacaaa cctctgcaac gccaggggta tacacgcggc gcagagtggt
     1261 caatgattca atcgggtatc gcgagcggat ggcgatcttg cctttttggt gcaactccaa
     1321 cacctcatcg cgcacttcga gaatctttgt cccttcgttg gtttccatcg ccgccaagac
     1381 cgcagctaga gatttttcgt cctctgcggt gatggtaatg tcccgaacaa catgccggga
     1441 ggtttccgtc aacaggttga tacttccgac atttccaccc gcctcagcga cagccgttaa
     1501 taatttaccc aacacccctt ttttctgtga atttcgaatg cgcaccttgc gcatgatctt
     1561 cggttcccac gccatggtta tctccttttt ggttatgctc attttaacgc gaataccgcg
     1621 aagtcgcttc gcggtattgg aacagacaac tttataaaga aatcgagttt acatcaataa
     1681 tttgtaaaac tcagcatatg agcggatcac gtcacattca gcatcaggga agagtccggt
     1741 tgggtcgaag agcacaggca aaagtccaga acgaagcgca ccgaccacat cggcaaaata
     1801 gttatcgcct acatacatcg tttcgctggc aactgtccct gagagttcca atgcgcgttc
     1861 aaagatacgg ggatgcggct tgaaagattg gacttctcca cctgtcagtg aaaaatggaa
     1921 atattctttc aatcctaatt cttcaagttc agagtgaaat ggttcttcgc ggttcgatac
     1981 cacaccgagc acataacctg ttctttgaag atgcttcaac aactcgtgga taccctcagg
     2041 tagataaggt tgcggtttgt aattcatgcc catgcaatca gaaacatgcg aagcaagttc
     2101 ttcggcttgt ggtttatgaa cgccaagcgc aaccaaacgc cgcttggtaa agttcaccca
     2161 aaatgcttta cggtcatcct taaagatttt agagtcttct tgaatctcaa gcgagcttgc
     2221 aaaatacaaa tgttcccaac gttcggctcg ggtttgatct tcttcagatg ggactagtcc
     2281 catcccttgg acacattccg caaacacatc tcgtgatgct ggttgattga ggcgcagggt
     2341 tccatcgagg tcgaaaagaa cagctttgat gtttttattt ggcattatta gcagagtctc
     2401 atatagtttt tgaaaatcag agggaaaatc taaaaggatt atatcaattt tattaaccat
     2461 tcttactcga caattgacgg aggatgccag ccatgctatg atgacatggt tattattctt
     2521 ttttttattc aggagttatg atgagttttg agtttattct tcgtattatt ggcatgatcg
     2581 tcttagctgt ttcgggtggt tttttgggat atgacctttc gcggttcaca cctgaagatg
     2641 cagttcgcac tgtgcttgtt ttcagcttgg ttgg
//
LOCUS       DAOREF010000415         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26909_length_2673_cov_3.325057, whole genome shotgun sequence.
ACCESSION   DAOREF010000415 DAOREF010000000
VERSION     DAOREF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26909_length_2673_cov_3.325057"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKI33_09210"
     CDS             <1..702
                     /locus_tag="PKI33_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM37230.1"
                     /translation="YFNGPLAYVVMLAILLPLGIYFSLILFYSSQQGLYGGAAAPDIT
                     PVNGLFAFLMIFLSPALTMRLLSDEARMGTLELLLTAPVRDSELVVGKWLGAMLFILT
                     LLGTTLIYPLVLNNFVDPGIDWMVVLSSYIGLILLCGTMLALGVGISAIFTNQFAAFF
                     VTLGLIFFLYFMIGLPAGLLPQSGEVFNYLSIQNHFDQMNSGTLTLGGIVYNLSLTVF
                     GLFAGFTAIETRRWR"
     gene            702..2324
                     /locus_tag="PKI33_09215"
     CDS             702..2324
                     /locus_tag="PKI33_09215"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNM37231.1"
                     /translation="MAGKKKNPNARYAVIALAVSLVACISTGLIGSANLLSNIGMFPL
                     SDEIQNGVNIALQVSIGLLIIALAVAAILNPDPVRRFFTGRQARYGSNSLILTIAVIG
                     ILIAGNYIVFKNTDLLGSPYDFTEDKSNTLAPETLQILAELKEPVVATGFFSTNSSTA
                     SAEELLAKFKNNSDGKFTYNFVNPDVDPVAAREAGITGDGKIMLQMGETKEIATFATE
                     DELARTLLRLMSPGARTVYFLQGHGEISLESGNELAYSVVQSTLEAKNYTVSTLNLLT
                     TREIPADALAIIVAGPQKPVSAEEVDLLKAYVDKGGSLVVLEDPIFFTKFGDAADPLA
                     EYLVNDWGIVLNNDVVIDGVNQQNPLAAISSLYNPDHPITQNLTENLFVIMPQARSIS
                     VSGEKENITQTWLISTTENSWGETSPLVENENPQFDPATDVQGPLYLALAAENSATTG
                     RVVVFGNSLFAIDGNFDVYGNGNFFINSVDWAAEQEDLLNLTTRPRTERIFTPPVETW
                     RFLLLVLVIVIIIPGMVVFFGVSAWLTRRKRG"
     gene            2328..>2673
                     /locus_tag="PKI33_09220"
     CDS             2328..>2673
                     /locus_tag="PKI33_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37232.1"
                     /translation="MATKSTKTTKSTKTTKTTKPRAPRAQATQKASKPAVRTGTWVAL
                     IAFIAVIATLIYMNRKAETEATATETPAAEETFIFSSDSVVSSIEVKPTEGNSVKVER
                     NEEKTWVLSQPEK"
BASE COUNT          636 a          733 c          607 g          697 t
ORIGIN      
        1 tactttaatg gaccgctcgc ttacgttgtg atgctggcga tccttttgcc gcttggcatt
       61 tacttcagtt tgatcctttt ctacagcagc caacaagggc tatatggtgg cgcagccgcc
      121 cccgatatca cccccgtcaa cgggttgttt gctttcctta tgatcttcct cagcccggcg
      181 ttgaccatgc gcttgctctc tgatgaagcc cgtatgggaa ccctggaatt gctgttgact
      241 gctcccgtcc gcgacagtga actcgtggtc ggcaagtggt tgggcgccat gctcttcatt
      301 ctcacattgc ttggcactac cttaatctat ccgctcgtgc tgaacaactt tgttgacccc
      361 ggcatcgact ggatggttgt gctttcatcg tatattggct tgatcctgct ttgcggcacc
      421 atgttggctt tgggtgttgg catctctgcc attttcacga accagtttgc tgccttcttt
      481 gtgaccctcg gcttgatctt cttcttgtac ttcatgatcg gtctgcctgc agggttgctc
      541 ccccaaagcg gtgaagtctt caactacttg agcattcaaa atcactttga ccaaatgaat
      601 tccggcacac taacactcgg cggcatcgtc tataacttga gcctgacggt gttcggtctc
      661 ttcgccggtt ttaccgcaat tgaaactcgg agatggcgat aatggctggg aaaaagaaaa
      721 atccaaacgc aagatacgca gtcatcgcgc tggcagtttc tctggtcgca tgtatttcga
      781 ccggtttgat cggctctgcg aatctgcttt ctaacattgg catgttcccg ctttctgacg
      841 aaattcagaa cggcgtgaac attgctttac aagtcagcat tggtttattg atcattgctc
      901 tggcagtcgc cgcgattttg aacccggatc ccgtccgccg cttctttacc ggacgccaag
      961 ctcgctatgg ttcaaattcc ttgatcctca cgattgccgt gatcggtatc ctcattgctg
     1021 ggaactacat cgtcttcaag aacaccgact tgctcggttc tccttacgat tttaccgaag
     1081 acaagagcaa tacccttgct ccagaaaccc tgcaaatttt ggctgaatta aaagaacctg
     1141 tcgtagctac aggctttttc tcgacaaatt caagcacagc cagcgcagag gaattgctcg
     1201 ctaaattcaa gaataacagc gacggcaagt ttacctacaa cttcgtgaac ccagatgttg
     1261 accccgttgc cgctcgcgaa gctggtatta caggcgacgg caagatcatg ttgcaaatgg
     1321 gcgaaacaaa agaaatcgct acctttgcta cagaagatga acttgcgcgc acccttttgc
     1381 gccttatgag ccccggcgca cgcacggtct atttcttgca gggacatggc gagatcagcc
     1441 ttgaatctgg caacgaatta gcctacagcg ttgtacaaag tacccttgag gcaaagaatt
     1501 acaccgtcag cacgcttaat ctgttgacga ctcgcgaaat ccctgcagac gctttggcaa
     1561 ttatcgttgc gggtccgcaa aaacctgtca gcgcagaaga agtggacttg ctcaaagcct
     1621 atgtggataa gggcggttcc ttggtcgtcc tcgaagaccc gatcttcttc accaagtttg
     1681 gtgatgccgc agaccccttg gctgaatacc tcgtcaatga ttggggcatt gttctcaata
     1741 atgacgttgt gatcgatggt gtcaaccagc aaaatccact ggcggcgatc tcttctttgt
     1801 acaaccccga tcaccccatc acccaaaacc tgaccgaaaa tcttttcgtc atcatgccac
     1861 aagctcgcag catcagtgtt tctggtgaaa aagaaaatat cacccaaact tggctcatca
     1921 gcaccacaga aaactcctgg ggcgaaacct ctcccttggt agaaaacgag aatccacaat
     1981 tcgaccccgc aactgacgtt caaggtcctt tgtacctcgc ccttgctgcg gagaattctg
     2041 cgacgaccgg tcgtgtggtt gtgttcggta actcactttt tgctattgac ggaaacttcg
     2101 atgtgtatgg caacggcaac ttcttcatca attctgtaga ttgggctgcc gaacaggaag
     2161 accttctgaa cctcaccacc cgtccacgca ccgaacgcat cttcacacct ccggttgaaa
     2221 cctggcgctt cctgctcttg gttctcgtga tcgtgattat catccccggt atggttgttt
     2281 tctttggcgt gtctgcgtgg cttacccgtc gtaaacgagg ttaattcatg gctacgaaat
     2341 ccaccaaaac aacgaaatct acaaaaacaa ccaagaccac caaacctcgc gcacctcgtg
     2401 cccaagccac acaaaaggct tccaaacctg ctgtgcgtac cggcacatgg gtggctctga
     2461 tcgctttcat cgctgtcatt gccactttga tctacatgaa ccgcaaagca gaaacagaag
     2521 ccacggcaac cgaaaccccg gcagcagaag agactttcat tttctcaagc gacagcgttg
     2581 tgagcagtat tgaggtaaaa cccaccgaag gcaattcggt taaggttgaa cgaaacgaag
     2641 agaaaacctg ggtgttatct cagcctgaaa aag
//
LOCUS       DAOREF010000416         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26943_length_2671_cov_4.466743, whole genome shotgun sequence.
ACCESSION   DAOREF010000416 DAOREF010000000
VERSION     DAOREF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26943_length_2671_cov_4.466743"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKI33_09225"
     CDS             complement(<1..628)
                     /locus_tag="PKI33_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNM37233.1"
                     /translation="MSDLIKQITGDLQKQIEGFKPEFNVSEAGVVLEAGDGIARVHGL
                     ANIKAQELVQFANGVIGVAFNLEKDNVGVIVMGAYEGITEGMSVKGLGRIASVPVGDA
                     MIGRVVNALGEPIDGKGPIATTGFRPIERIAPGVVERQDVDTPVQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTAVAIDAIINQKGKGVTCIYVAVGQKKAAIARTL"
     gene            872..2008
                     /locus_tag="PKI33_09230"
     CDS             872..2008
                     /locus_tag="PKI33_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37234.1"
                     /translation="MNTRRPSKRQWKIAVLANIKDDNQPKPDNVPPDAFADFDHIETI
                     DSLRAALETDGHTTVFVHADRELPYSLRKVKPDICFNIAEGLGGDAREAQVPALLEML
                     AIPYTGSRVLANGISLDKTLTKRIWRDRRLPVAPFQEFQFGDEPLRPELKFPLFVKPA
                     REGTGMGVDMKAIVNNETELRERAEYIINTYQQPALVETFLPGREFTVGILGRADAKL
                     YSRHPEWYEKDGFHRFPVLELDTGNTEGPWVYTNEAKSKDVGPEGNEGFFCPANIDPE
                     LEKKLKHLAFRAHNILNVLDVSRTDIRLDEEGNPRLIEINTLPGLTPNYSDLCLQAAA
                     EGISHTDLVLDILYLAAGRWGLLEPREISTESGKTGPLSYRLGR"
     gene            complement(2069..>2671)
                     /locus_tag="PKI33_09235"
     CDS             complement(2069..>2671)
                     /locus_tag="PKI33_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37235.1"
                     /translation="CHDGVDRFDDFNHNAYAFKLEGEHANVACTKCHLDARSIADLQS
                     APQDCYSCHRQDDVHNGENGTDCETCHNPSNWEDADFDHSRTNFPLTGGHSNVECNQC
                     HTNGTYKGLSSTCVTCHQDPAFHAGAFGTDCESCHSINAWSPAQFNLSHPEPRVDEGG
                     SGIHHGGASCRECHPSSVFESSCDSCHSGGFEGGEGGDDD"
BASE COUNT          692 a          716 c          628 g          635 t
ORIGIN      
        1 caagggtacg agcaatggca gcttttttct gacccactgc cacatagata caagtaacac
       61 ccttgccctt ctggttgatg atcgcatcga tagcgacagc ggtcttacca gtctggcggt
      121 cgccgatgat caattcgcgt tgaccgcgac cgatcgggat catcgagtcg atggatttga
      181 taccggtctg aaccggggta tccacatcct gacgttccac cacgccggga gcaatgcgct
      241 caattgggcg gaagcccgtg gttgcgatag gacccttacc gtcaatgggc tcgcctaagg
      301 cgttgaccac acgtccgatc atggcatccc ctaccggtac agaagcgatg cgtcccaacc
      361 ccttaaccga cataccttcg gtaatacctt cgtacgcacc catcacgatc acaccgacgt
      421 tgtccttttc caagttgaac gcaacgccga tcacaccgtt ggcaaattgt acgagttcct
      481 gtgccttgat attcgcaaga ccatgaacac gagcaatgcc atcacccgct tcaagaacaa
      541 caccagcttc gctgacgttg aattcgggct tgaatccctc aatttgtttc tgcagatcac
      601 cagtgatctg cttgatcaag tctgacattc ggtagcctcc accaaaaaaa ttcgttttac
      661 gtacaacaac ccggataagg ggcttatccg ggcaaaatat tcaactcttg gcacttcgtg
      721 agtaagcgca caagtgggct aatgatacct gaaaggtgga cgattgtcta gttactcccc
      781 aaacgggtga agaaatgcac tttccccttg tcatccatac atcttatact atactacaag
      841 ggtgaaattt acaggagcga cccacacata gatgaatact cgccgtccgt cgaaacgcca
      901 atggaagatt gcagtacttg ccaatattaa ggatgacaac cagcccaaac cggataatgt
      961 tcccccggac gctttcgctg actttgacca tatcgaaacg attgactcgc ttcgcgccgc
     1021 gctcgaaacg gatggtcaca ctactgtctt tgtccacgcg gatagagaac tcccctacag
     1081 cctccgcaag gtcaagccag atatctgctt caatattgcc gaaggtcttg gaggtgacgc
     1141 ccgtgaagca caagtgccag ctttactgga aatgctcgcc attccataca ccggttcccg
     1201 tgtgcttgcc aatggcatct cacttgataa aacactgacc aaacgtatct ggcgtgaccg
     1261 tcgtctgcct gtggctccct ttcaagaatt ccaatttggg gacgaaccct tacgccccga
     1321 gctgaaattc ccgctcttcg tcaaacctgc ccgcgaaggg acaggcatgg gcgtagacat
     1381 gaaagccatc gtcaataacg aaacagaatt gcgcgaacgt gcagaataca tcatcaacac
     1441 ctatcagcaa cctgctctcg ttgaaacctt tttgcccggg cgcgaattca cagtgggcat
     1501 cttgggccgt gccgatgcca agctatattc tcgtcacccc gaatggtatg aaaaagatgg
     1561 cttccaccgc ttccctgttt tggaattaga cacaggcaat acggaaggtc catgggttta
     1621 tacaaatgaa gctaaatcaa aagatgttgg tcctgaaggc aacgaaggtt ttttctgccc
     1681 agccaacatt gacccagaac ttgagaaaaa attaaagcac cttgcctttc gtgctcataa
     1741 cattctcaat gtccttgatg tctctcgcac agacattcgg ttggacgaag aaggcaatcc
     1801 acgcctgatt gagatcaaca ccttgccagg gcttacccct aactatagtg acctctgctt
     1861 acaagctgcg gcagagggca tcagccatac agacttagtg ctggatattc tctatctcgc
     1921 tgctgggcgt tgggggcttc ttgaacctcg tgagatttca actgaaagcg gaaaaacagg
     1981 tcccctatcg tatcgtttag ggcgataagt ccgcataaaa aaagagcgct agggtagcct
     2041 agcgctcttt ttttaatggc aaagactatt aatcgtcgtc tccgccttcg ccaccttcaa
     2101 acccaccgct atgacaagag tcacagctag actcgaagac agatgagggg tgacattcgc
     2161 ggcaggaggc tccgccgtgg tggataccgc taccaccttc gtcaacgcgc ggctcagggt
     2221 gagacaggtt gaattgggca ggagaccaag cattgatcga gtggcaagac tcgcaatctg
     2281 taccaaaggc accagcatga aacgcagggt cttgatggca ggtgacacag gtagatgaaa
     2341 gccctttata cgtcccattg gtatggcatt gattacactc cacgttagag tgtccgccag
     2401 ttaatgggaa gttggtgcgg ctgtggtcaa aatcagcatc ttcccagttc gatgggttat
     2461 gacaggtttc acagtctgta ccattctcac cattgtgaac atcgtcttgt ctgtggcagg
     2521 agtaacaatc ttgcggtgca gattgcaggt ctgctataga gcgtgcatcg agatgacatt
     2581 ttgtacaagc gacgttagca tgctcacctt ctagcttgaa agcataagca ttgtgattga
     2641 aatcatcgaa gcgatctacg ccatcatgac a
//
LOCUS       DAOREF010000417         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26980_length_2669_cov_3.852716, whole genome shotgun sequence.
ACCESSION   DAOREF010000417 DAOREF010000000
VERSION     DAOREF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26980_length_2669_cov_3.852716"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKI33_09240"
     CDS             complement(<1..330)
                     /locus_tag="PKI33_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37236.1"
                     /translation="MNDPLALIIEDDEDLAEIFGQALTAAGYATEIVRDGLAAQTRIK
                     DVTPAIITLDMHIPQISGDRILQQIRSDQRLLKTRVVVTTADAIMAENARGIADLVLI
                     KPITFSQL"
     gene            complement(323..2062)
                     /locus_tag="PKI33_09245"
     CDS             complement(323..2062)
                     /locus_tag="PKI33_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM37237.1"
                     /translation="MIANLQNNWERINKFDGFDQDIARRGRLLNVLLLGMSILCLLGI
                     IAIAVVVTMNGAWNKPGNVTTIVAAAVFLIGAIGLGYVNQYSVRLASFLFLALLMVVF
                     IFSDDPAQLANGRSSFVFFIPVAVASLLLTPASSFIFAIAGVAIMIWLANEGNVLPNV
                     STISGEFMFALVSWLSARSLEQALVDLRATNQNLDQLVEQKTRELANTLSRELIVAGR
                     NQAILNSIADGVIVFDERNMAILANPAISRLTNIPVSELVDRSMVDFILRDALSESRD
                     SLQQIFEDPEEETITGVRVNWGSKTLSASVARVNDADDKVIGTVAVFRDITREVELEK
                     MKDTFMGIVSHELRTPLNAILGYAEMLKEGIYGAVNEKQANIAERVMNSTQRLLSIVS
                     DLLDQAQIQSGKLKIRMMSCNPIEMLESLHGVMDRIALEKGIEFRTDIDPAMPTVIMG
                     DPQRLQQVMINLANNAIKFTEKGYVSVKILRMDADHWQIRTTDTGSGIPTDAQEYIFE
                     TFRQVESSAIRQHGGVGLGLSIVKQLVELMKGKITLESEIGKGSTFTVTFPIVINDDS
                     QPTVTDPDRRNYE"
     gene            complement(2119..>2669)
                     /locus_tag="PKI33_09250"
     CDS             complement(2119..>2669)
                     /locus_tag="PKI33_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37238.1"
                     /translation="NAILSFEEEEYFRCYQLEVGISPSVNIHALMTLRDFDYPLEHPS
                     VRKVFQFLETQKSGDFWVDKWNSSSFYTSSHYVIACAKFKNAIAQPTVDWILKNQRAD
                     GSWGTFGSTTEETAYCLQALSIWSRHVRSVPQDVIRRGRTWLLEHYDLPHSPIWIGKG
                     LYTPQWVSCSAILSAMLLTVSY"
BASE COUNT          761 a          658 c          590 g          660 t
ORIGIN      
        1 caattgagaa aaagtgatcg gtttgatcaa gacaagatct gcaatgccac gggcattttc
       61 tgccatgatc gcatctgcag tcgtgacgac cacccttgtc tttagcaaac gttgatcgct
      121 ccgaatttgt tgaaggatgc ggtcaccgga tatttgcggg atatgcatat ccagagtgat
      181 aatagcgggc gttacatctt tgatgcgggt ttgagcagca agcccatcac ggacaatctc
      241 tgtggcatat ccggcagcag taagtgcctg cccaaagatc tcggcgaggt cttcatcatc
      301 ttcaataatc aacgcgagag ggtcattcat agtttctcct atctggatct gtaacggtgg
      361 gttgcgaatc gtcattaatt acgatcggga aagtgaccgt aaacgtactg cctttcccaa
      421 tttcactctc caaggtgatc tttcccttca tcaattcgac aagctgtttg acgatggaaa
      481 gcccaagccc aaccccgcca tgttgacgaa tagcactgct ttctacctgg cggaaggtct
      541 caaaaatata ttcctgtgca tcagtaggga tgccactgcc tgtatcggtg gtacggatct
      601 gccaatgatc tgcatccatg cgcaggatct tgacactgac atatcctttt tctgtaaatt
      661 tgatggcatt gtttgcaagg ttaatcatca cctgctgcaa ccgttgtggg tctcccataa
      721 tgaccgttgg catggcgggg tcaatatccg tgcggaattc aatccccttt tccaaagcga
      781 tacggtccat cacaccatgc agggactcta acatctcaat agggttgcag gacatcatgc
      841 ggatcttcaa cttgcccgat tgaatctgtg cttgatctaa caaatcacta acgatgctca
      901 gcaaacgttg ggtgctgttc atcacacgct cagcgatatt agcttgtttc tcgttgaccg
      961 caccgtagat accctctttc aacatttcag cgtatccaag aatagcgttc aacggggtgc
     1021 gcaattcatg agatacgatg cccatgaagg tatctttcat tttttcaagc tctacctcac
     1081 gagtaatatc acggaaaact gccacagtac cgatcacttt atcatcggca tcgttcacac
     1141 gcgccacact ggcagagagg gtcttactgc cccagttgac acgaacaccg gtgatggtct
     1201 cttcttcggg gtcttcaaat atctgttgca agctgtcacg cgattcagaa agggcatcgc
     1261 gcaaaataaa atccaccata ctgcggtcga caagttctga aacgggtata tttgtgagac
     1321 ggctgatagc agggttggca aggatcgcca tattccgttc atcgaaaacg atcacgccat
     1381 ctgcaatcga gttgagaatc gcctggttac gtccagccac gattaattca cgagaaagag
     1441 tattggcaag ttcacgagtc ttttgctcta ctaactgatc aaggttttga ttggtggcac
     1501 gcaggtcaac cagggcttgt tccagtgagc gcgccgaaag ccatgaaacc agcgcaaaca
     1561 taaactcgcc agaaatcgtg gacacatttg gcaaaacatt gccttcattc gccaaccaaa
     1621 tcataatagc cacacctgcg attgcgaata taaaactgct ggcaggggtg aggagcaaac
     1681 tggcaaccgc tacagggata aagaacacga atgatgaacg tccatttgca agctgagcag
     1741 ggtcatcact aaagataaac acaaccatca acaatgccaa aaacaagaaa gacgcaaggc
     1801 gcactgaata ttgattgaca tatccaagcc caatggcacc gatcaaaaag accgccgccg
     1861 caacgatggt ggttacattc ccgggtttgt tccaagcacc attcatagtc actactacgg
     1921 caatagcaat aatccccagc aaacaaagta tggacatgcc caacaggagc acattaagca
     1981 ggcgaccacg ccgggcaata tcctggtcaa acccatcaaa cttattaatt cgctcccaat
     2041 tgttttgtag attcgcgatc ataggcacca ccatgtttac gtagagaata cagacaaaat
     2101 gattaaaaca taaaacagtt aatatgaaac cgttaacaac attgcactta agatagctga
     2161 acaagagacc cattgcgggg tgtacagtcc cttgccgatc caaatcgggg aatgcggcaa
     2221 gtcgtaatgc tcaagcaacc aggttcttcc tcgtcgaatc acatcttgcg gtacgctacg
     2281 gacgtgcctc gaccatatgg aaagcgcctg aaggcagtag gctgtttctt cggttgtaga
     2341 gccaaacgta ccccaagagc cgtctgcgcg ttgattcttt agtatccaat ccactgtcgg
     2401 ctgagcaatg gcattcttaa acttggcaca ggcaatcaca taatgagacg aggtatagaa
     2461 tgaagaagaa ttccatttat cgacccaaaa gtctccagat ttttgggttt ctaaaaactg
     2521 aaacactttt ctgacgctgg gatgttcgag cggatagtca aaatccctta atgtcattaa
     2581 cgcatggata tttacactgg gagagatacc cacctccaat tgataacagc ggaagtactc
     2641 ttcttcttca aaagacaata tggcattaa
//
LOCUS       DAOREF010000418         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_26999_length_2668_cov_4.577497, whole genome shotgun sequence.
ACCESSION   DAOREF010000418 DAOREF010000000
VERSION     DAOREF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26999_length_2668_cov_4.577497"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKI33_09255"
     CDS             <1..270
                     /locus_tag="PKI33_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37239.1"
                     /translation="QQRVAIARALANDPDILVADEPTGSLDSVTADHIFDVFSRLANE
                     RGKTIVMVTHDMGLKSRFSRCLTLVDGELQNEIKADAKPKRSRKQ"
     gene            267..1460
                     /locus_tag="PKI33_09260"
     CDS             267..1460
                     /locus_tag="PKI33_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM37240.1"
                     /translation="MTQPKKKRTPSHAGSPRTQPKAAPQKVHDASPEAIIEMRGVVKK
                     FSNAAGEFTVLKGVDLMINRGEFVSIVGKSGSGKSTLLNMITGIDHPTSGQVIVNHND
                     IYTGVSESARSKWRGKNLGIVFQFFQLLPMLTLLENVMLPMDYVDMYDFDERPIRALE
                     LLELVGLEKFANKLPVLVSTGQQQLAAIARAMACDPPLLIADEPTGNLDTRSSDTIIK
                     LFQHFVDQGKTIVMVTHDPSLTSRTDRNIIISDGEIIDETISRSLPQLRHRHMLEFSK
                     LAQRRMYQPRETIISRDEPVKNFFMIRSGQVEVVLQKQRSKETVLSRLGPNEFFGDIE
                     LLRGGKSIANVRAGSEPVEVVIIPREDFTRVMDESPITVEAISKIVQRRLEQHRMVDP
                     RKSRS"
     gene            1471..>2668
                     /locus_tag="PKI33_09265"
     CDS             1471..>2668
                     /locus_tag="PKI33_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37241.1"
                     /translation="MNADPRRLPQVLYIADSDESRSLVRRLLAYTFVVLEAANPLDGL
                     GLAEETRPNLILLDTNVAQMTSSEAATRLKKMLSGTPIVVVSGDTSNKARARALAAGA
                     VGFIPKPIGDDFEQLVIEYLNGRTDELENAEQYLREYQLELAEKIEDQTRQLTNTVER
                     NKYLLSQNQKMFAMLERRHKLLETAARVGQMVTSILDMTELLRHTVNIICSEFHFYYS
                     GIFLISDDRKWAVLRAGFALAGRKMLDADYKLPIDDKSMIGRSILTQQAQIALDVAGE
                     ESRFKNPYLPNTRSEMALPLIVNSISVGAVTIQSSELNAFNEEDITSLQAMADQVAIA
                     INNANLMKDLASANAELLRTKTYEAIATATGEAIHWVGNKAAPIPGSVHRVREDVQYL
                     LALVAQL"
BASE COUNT          708 a          619 c          655 g          686 t
ORIGIN      
        1 cagcagcgtg ttgccattgc acgtgctctg gcaaatgacc cggatattct cgtcgcagat
       61 gaacccaccg gcagcctcga ctctgtcacc gcagaccaca tctttgatgt cttttcgcgt
      121 ctggcaaatg aacgtggcaa gaccattgtg atggtaacgc atgacatggg cttaaagagc
      181 cgcttttctc gttgcttgac tctcgtcgat ggtgaattac aaaatgaaat caaagcggat
      241 gccaaaccga agaggagccg caaacaatga cgcaaccgaa gaagaaacgc actccctcgc
      301 atgccgggtc gcctcggact cagccgaaag ctgctccaca aaaagtgcat gatgcctcac
      361 cggaagcgat catcgaaatg cgcggcgtgg ttaagaaatt ttccaatgcc gctggtgagt
      421 ttaccgtcct caaaggcgta gacttgatga ttaaccgcgg tgagtttgtg tctattgtgg
      481 gtaaatctgg cagcggtaaa tcgacgctat taaatatgat cacaggcatt gaccatccca
      541 ccagcggaca agtcattgtc aaccacaatg atatttatac cggtgtgagt gagagcgctc
      601 gctcaaaatg gcgcggaaag aacttgggca ttgttttcca attcttccaa ttactgccca
      661 tgttgacctt actcgaaaac gtcatgcttc cgatggatta tgtggacatg tacgattttg
      721 atgaacgtcc tatccgcgct ttggagcttt tggaattagt aggtttggag aagttcgcta
      781 acaaactacc ggttttggtc tcaacgggtc aacaacagtt agcagcgatt gcacgtgcca
      841 tggcatgtga ccctccgcta ctgattgcag atgaacccac cggcaacctc gatactcgtt
      901 cttctgatac catcatcaaa ttgtttcagc attttgtcga ccaaggcaag accattgtga
      961 tggtgaccca tgacccatcc ttaacctcgc gtacagaccg taacatcatt atctctgacg
     1021 gcgaaattat tgacgaaacg atttcccgct ctttgccgca acttcggcat cgccacatgc
     1081 tggagttttc caaacttgcg cagcgccgaa tgtatcagcc gagagaaacc attatttctc
     1141 gcgatgagcc cgtgaaaaac tttttcatga tccgtagcgg acaagtagaa gtcgttttac
     1201 aaaagcaacg cagcaaagaa accgtccttt cgcgcttggg tcctaatgaa ttttttggtg
     1261 atattgaact cttacgcggc ggcaaatcga ttgcgaatgt gcgcgcagga agcgagccgg
     1321 ttgaggtcgt gatcattcca cgtgaagatt tcacgcgtgt tatggatgaa tctcctatca
     1381 ccgtagaagc catcagcaag atcgttcaga gacgtttgga acaacatcgc atggtagacc
     1441 caagaaagag ccgctcgtag atttgctcct atgaatgctg accctcgcag actcccgcaa
     1501 gttttatata tcgccgatag tgacgaatcc cgttcgcttg tgcggcgttt gcttgcatat
     1561 acctttgttg tgttagaggc tgcgaatccg ttagatgggt taggactggc tgaagaaacc
     1621 cgtccaaatt tgattttgct agataccaat gttgctcaga tgacaagcag tgaggcagca
     1681 actcgcctta agaaaatgtt gtctggtact cccattgtgg ttgtttctgg agatacatcg
     1741 aataaggcac gtgcgcgggc tttggcggct ggagcggtgg gcttcattcc caagccaatt
     1801 ggcgatgact ttgaacaatt ggttattgaa tacctgaacg gcagaaccga tgaattagaa
     1861 aatgctgaac agtatttgcg tgaatatcaa cttgaattag ccgaaaagat cgaagaccag
     1921 acccgtcaac taacgaatac agttgaacgc aacaaatatc tgctttcaca gaaccagaag
     1981 atgtttgcca tgcttgagcg cagacataag ttattggaaa cggcggcacg agtagggcag
     2041 atggtcacat ctattctgga tatgacagaa ctacttcggc acaccgtcaa tattatttgt
     2101 agtgagttcc atttctatta ttctggaatt ttcctgatct cagacgaccg taagtgggca
     2161 gttctgcgag cagggtttgc attggctggg cgcaagatgc tggatgccga ttacaagttg
     2221 ccgattgatg ataaatccat gatcggacgc tcgattttga cacaacaagc tcagattgca
     2281 ttggatgtgg cgggtgagga gtcacgcttc aagaacccgt atttgccgaa tactcgctca
     2341 gagatggcat tgccacttat tgtcaattcg atctctgtcg gtgcggtgac cattcaaagt
     2401 agtgagctta atgctttcaa cgaagaggat attacgtcct tacaagccat ggcagatcaa
     2461 gtggcaattg ctatcaacaa tgccaacctc atgaaagacc ttgcttctgc taatgcggag
     2521 ttgttgcgca caaagacata tgaagcaatt gctaccgcaa caggcgaagc aattcactgg
     2581 gtagggaata aggcagcgcc gatcccagga agtgtgcatc gtgtgcgtga agatgttcaa
     2641 tacttattgg ctttggttgc gcagttaa
//
LOCUS       DAOREF010000419         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27071_length_2664_cov_4.982369, whole genome shotgun sequence.
ACCESSION   DAOREF010000419 DAOREF010000000
VERSION     DAOREF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27071_length_2664_cov_4.982369"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKI33_09270"
     CDS             complement(<1..359)
                     /locus_tag="PKI33_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNM37242.1"
                     /translation="MTLNINEIRKDFPILERETANGKRVIYLDSTATSQKPLQVIEAM
                     NDYYRRSNANIHRGVHTLAEEATSLYEGARERIAKFIHAATSREIIYTRNTTESINLV
                     AYSWARANLKAGDLVILT"
     gene            complement(437..1042)
                     /locus_tag="PKI33_09275"
     CDS             complement(437..1042)
                     /locus_tag="PKI33_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37243.1"
                     /translation="MAVKSKTKSNSKTKVVVKAKTATKKGTVKVSAARKAVSKSKTKV
                     VAKSVKPSKSVVSSIKKKPASAEKPVEVTRTINLSSQIRQPAKPVLRRSSVGLGATNS
                     NPFTFMKAPRPEYAFEVGDTVEVFCDHEKGRERVRGWIKGIVVQVDNKMVAVQFRSNV
                     FLTDGWMVPDRILWYPLNSEHIRPGTTGKKPALKKDIIPDY"
     gene            complement(1177..2514)
                     /gene="sufD"
                     /locus_tag="PKI33_09280"
     CDS             complement(1177..2514)
                     /gene="sufD"
                     /locus_tag="PKI33_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNM37244.1"
                     /translation="MQEKPKVTVSRGRRADTAAKEFTFTQADVPSANGLASFRAGAWD
                     LFSKQNLPDTSLEAWRRTDLRLLPAPDFKLPKAGAFEDLPAVPEELLKPLTGEQHGGQ
                     ITLLPGGSKVDLDEALAKKGVVFTDFRTAEKNHSDLLKKLIGQIVKPDDGKFAALASA
                     LSENGVLLYVPKGVQVEQPLHSVLWGPGTDLAHFSHLLVYVEDGASVTYVHEAASPDE
                     TGHAMHAGIVEIYVGKDANLRFVELQSWGRHVWNFSHERARVDRGGNLDWIFGAVGSR
                     LTKNFSDLDLIGEGAQGRMSGFYFTDGTQHLDHDTQQNHMAPHCTSDLLFKGALKGKS
                     RSVWQGMIYVAPGAQKTDGYQANRNLVLDDQARADSIPGLEILADDVRCTHGATVGKL
                     EQEPLFYLKSRGIPQKEAEQLVVEGFFDPIMQRIPFEGVRERFQQAIQIKLTA"
     gene            complement(2516..>2664)
                     /locus_tag="PKI33_09285"
     CDS             complement(2516..>2664)
                     /locus_tag="PKI33_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNM37245.1"
                     /translation="LMSRGLSEEEATTMVVSGFIEPLVKELPMEYAVEMNRLIQLQME
                     GSIG"
BASE COUNT          651 a          670 c          660 g          683 t
ORIGIN      
        1 gtcagaataa caaggtcgcc cgcctttaaa tttgcacgcg cccaagaata agcgacaaga
       61 ttaatcgact cggttgtatt acgcgtgtaa ataatctcac gtgaagttgc cgcatggata
      121 aatttggcaa tgcgttcacg cgcaccttca tacaaagaag tggcttcttc agccaaagta
      181 tgcacaccac ggtgaatatt tgcatttgaa cggcgataat aatcgttcat cgcttcgatc
      241 acttgcaaag gcttctgaga agttgcggtg gaatcaagat aaatcacgcg cttgccattg
      301 gcagtttcgc gctctagaat agggaaatct ttacgaattt cgtttatgtt tagcgtcatg
      361 ggaaataaaa aatagacagg tacacaagta cacatgtcta ttttttgctt gtgtacctgt
      421 ctacctgcgt attggcttaa taatccggaa taatatcttt cttcaaggcg ggtttcttgc
      481 cggtagttcc agggcgaatg tgctcagaat tcagcgggta ccaaagaatg cgatctggca
      541 ccatccaacc gtctgtgagg aaaacgttcg agcggaactg cactgccacc attttgttat
      601 ccacttgaac tacaatgcct ttgatccaac cacgcacacg ttcgcgtccc ttttcgtgat
      661 cacagaacac ttctaccgta tcacccactt caaaagcata ctcagggcgt ggcgccttca
      721 taaatgtgaa cggattggag tttgtcgcac ccaacccaac agaagagcgg cgcaagacag
      781 gtttcgcagg ctgacggatc tggctcgata agttaatggt gcgcgtcact tcaaccggtt
      841 tttcagcaga agctggcttc ttctttatag aagaaacaac cgacttacta ggcttgacac
      901 tttttgcaac cactttagtc ttcgattttg aaactgcttt acgagccgca ctaaccttaa
      961 ccgtcccctt tttggttgcc gttttcgctt ttaccaccac ttttgtcttc gaattggatt
     1021 ttgttttaga cttcactgcc atagctcact tcctacgaac gaaaattgag acgccctccg
     1081 cctcattctt aaaggatgcc tgatgcaacg cttcaggcat tttcttacgg tgataatgct
     1141 ctcgctgggg tgtaagcccc agcgagagaa aacaaattac gctgtcaact tgatctgaat
     1201 cgcctgctgg aagcgctcac gcacaccttc aaacgggatg cgttgcatga tggggtcaaa
     1261 gaaaccttcc accaccaact gctccgcctc tttttgcgga ataccacgag atttcaggta
     1321 aaacaggggt tcctgttcaa gtttaccaac ggttgcgcca tgtgtacagc gcacatcgtc
     1381 ggcgagaatt tcgagacccg gaatagaatc cgcgcgtgcc tggtcatcga ggacgaggtt
     1441 acggttggct tggtagccat ccgtcttctg ggcaccgggg gcaacataga tcataccctg
     1501 ccacaccgag cggctctttc ctttaagcgc acctttgaac aaaaggtcgc tggtgcagtg
     1561 cggagccata tgattctgct gggtgtcgtg gtcgaggtgc tgggttccat cggtaaagta
     1621 gaagccagac atgcgtccct gcgccccttc cccgatcagg tctaggtctg agaagttctt
     1681 ggtgaggcga gagcctacag caccgaagat ccagtcgagg ttgccgccac gatctacacg
     1741 ggcgcgttcg tggctgaaat tccacacatg cctaccccac gactgtaatt ctacaaaacg
     1801 caggttggca tctttcccta catagatttc gacaattcct gcatgcatgg catgaccggt
     1861 ctcatcgggt gaagcggctt cgtgaacata cgtcactgaa gcgccatctt ccacgtaaac
     1921 gagtagatgc gagaaatgcg cgaggtcagt accgggtccc cacaacacag agtgaagggg
     1981 ttgttccact tgtacgccct tgggcacata aagaagaacg ccattttcag aaagcgcaga
     2041 agccaaggcg gcaaacttgc catcatctgg cttgacgatc tgaccgatca atttcttgag
     2101 taagtcagag tgattctttt ctgctgtgcg gaagtcagtg aagacgacgc cctttttagc
     2161 gagggcttca tctaagtcca ctttggagcc gccgggcaat aaagtgattt gtccgccgtg
     2221 ctgttcaccg gtcaacggct tgagtaattc ttctggaacg gctggcaaat cttcgaaggc
     2281 acccgctttg ggaagtttaa aatcaggtgc gggcaataaa cgcaaatcgg tgcggcgcca
     2341 ggcttcgaga gaagtgtcgg ggagattctg cttggagaat aaatcccagg caccggcgcg
     2401 gaaagaagcg agtccgttcg cggaagggac atctgcctgc gtaaaggtaa attcctttgc
     2461 ggcagtgtcg gcgcgacgtc ctcgagatac ggttactttt ggtttctctt gcatgttagc
     2521 caatcgatcc ttccatttgt aactggatga gtcgattcat ttcgactgcg tattccatgg
     2581 ggagttcttt gacgagcggt tcgatgaagc cagagaccac catggtggtg gcttcttctt
     2641 cactgagccc acgagacatg agat
//
LOCUS       DAOREF010000420         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27072_length_2664_cov_4.943273, whole genome shotgun sequence.
ACCESSION   DAOREF010000420 DAOREF010000000
VERSION     DAOREF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27072_length_2664_cov_4.943273"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..754)
                     /locus_tag="PKI33_09290"
     CDS             complement(143..754)
                     /locus_tag="PKI33_09290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNM37246.1"
                     /translation="MKEEIHITFLRHGRSRADDEEVHEGRYDSPLTDVGRSQVQARAH
                     EFLSRGFQFDKIFSSTLQRAQETALIIGQTLNVSIEKDSDWMEMDNGPLAGLSREVAE
                     KEYPRPIFRNPYEPFCGSGESDWEIYCRAAKAVEKIIRQGVGSYLVVAHGGILNSALR
                     TIVGTQPFVNQQGIVFGFGDTGYARLTYRPSEHLWYFREFNAG"
     gene            complement(957..2459)
                     /locus_tag="PKI33_09295"
     CDS             complement(957..2459)
                     /locus_tag="PKI33_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNM37247.1"
                     /translation="MRRLFILSAFFLFVLTSCGVPSTITNLPTKTPTELPSPTVSPTP
                     KPLLTVKVMSYNILWGAGVDREFDENLKAKGQDSQYGRDRLPELLSVIKELDPDILGI
                     QEGAGWDRGTPSVIQKVAEELNMNYFLAKGAASELNVMILSKFKIIETENLSPEVGNV
                     GALRVKLTTPDGQPLNVFVVHLDPFSANIRLCEINTLIQYMQPYFQQRTIVMGDFNTT
                     PNLWDHKNLKETGMTLVAIEQSWQIDQIWASPSMNWSKTDWFKSFLPPKNISDHHPIG
                     AEIAIFSNPISLPTVTPIPPTPVIKPIPLIYDFITDPQVLRFDNIEDACTKPKWNSDW
                     TTEKFSDGVIAIVGEDYWKAGVSRNKDFSEGQGVVLRFKFEKGTEANIFFDSGDWSTN
                     PYRRFGMNIRQDSVQTDLWQGKTGMGGKNLNGNFKPKPETWYNLMIVISKNGEFLEAV
                     WEPNDPSQVVTYHEQLGEKWTGLTWRFSVGANTGNVLIDDFSEIIFDKIK"
BASE COUNT          691 a          613 c          543 g          817 t
ORIGIN      
        1 gacatatctg ccgcctaacg gcatgcggct tgacctgcgg gtacctgccc tgtctcgcgg
       61 accgggcgaa gccgtccagc cagcctgtcc tgatgttctt cagggttgct gcatatcatc
      121 ccagttctat cgggacgctg tgttagcccg cgttgaattc acgaaaatac caaagatgtt
      181 cagaagggcg gtaagttaac cgagcatatc cagtgtcacc aaaaccaaat actatacctt
      241 gctgatttac aaatggctgt gttccaacaa ttgttcttaa agcggaattc aaaataccgc
      301 catgagcaac aactaaataa cttccaacgc cctgccgaat aattttttct accgcctttg
      361 ctgctcgaca atatatttcc cagtcgcttt cccctgaccc gcaaaaaggt tcgtaggggt
      421 tacggaaaat aggtctagga tattcttttt cggcaacttc tctcgacaaa cctgctagcg
      481 gaccattgtc catttccatc caatcagaat ctttttcaat tgagacattt agtgtttgac
      541 caataattag tgctgtttcc tgagcgcgtt gcagtgtgct tgaaaagatt ttatcaaatt
      601 gaaaaccacg agagagaaat tcatgagctc ttgcttgaac ctgagaacgc cccacatcag
      661 ttagtggaga gtcgtatcta ccttcatgga cctcttcatc atcagcgcga gaccgtccat
      721 gccttaggaa ggttatatga atttcttctt tcatcattta ctccgatgcg cgaagcggac
      781 taacggcata cgacttgccc tgtggagacc cgccctgtct cgtggaccgg gcgaagccgc
      841 ccagccagcc tgacctgaga cggtctgggt ttccctttaa aggtgctttt gctcttgact
      901 ttcaagacag ttgctgcacg ctttgtgcca aaaaccaatc cttttgaagc ctgtcattac
      961 tttatcttgt caaaaatgat ttcactaaaa tcatcaatca atacattgcc tgtatttgct
     1021 cctactgaaa atcgccaagt tagtccagtc cacttttcac ccaattgctc gtggtatgtg
     1081 actacctgag aagggtcatt tggttcccag actgcttcca gaaattctcc gtttttagat
     1141 ataacaatca tgaggttgta ccaagtttca ggcttgggtt tgaaattccc attaagattt
     1201 ttgccaccca ttcctgtttt gccttgccat aaatccgtct gaaccgaatc ttgtcgaatg
     1261 ttcattccaa atcttcgata cggatttgta ctccaatccc cgctgtcaaa gaatatattg
     1321 gcttctgtcc ccttctcaaa tttaaagcgt aatacaactc cttgaccttc tgaaaaatcc
     1381 ttgtttcttg agactcctgc tttccagtaa tcttcaccta cgatagcaat caccccatca
     1441 gaaaatttct cggttgtcca atctgaattc catttgggtt ttgtacaagc gtcttcaatg
     1501 ttgtcgaagc gtaacacttg cggatcagtt ataaaatcgt aaatcaatgg tattggcttg
     1561 ataactggcg tcggtggaat tggagtaact gttggtaacg atattgggtt agaaaatata
     1621 gcaatttcag cgccaatagg atgatgatca gatatatttt ttggagggag aaaggattta
     1681 aaccaatctg tttttgacca attcatagag ggggatgccc atatttgatc tatctgccaa
     1741 gattgttcaa tcgctactag tgtcatgcca gtttccttca ggtttttatg atcccataaa
     1801 tttggtgtag tattaaagtc ccccattact attgttcgtt gttgaaaata tggttgcata
     1861 tattgaataa gtgtatttat ttcacataat cggatgtttg ctgaaaatgg atcaagatgc
     1921 actacgaata catttaaagg ctgcccatca ggagttgtca atttaactcg tagtgcgcct
     1981 acattgccta cttctggtga taaattctct gtttcaataa ttttgaattt gctgagaatc
     2041 atcacattga gttcgcttgc cgcacctttt gctaaaaagt agttcatatt gagttcttcg
     2101 gcaacttttt ggataacaga tggtgtgcct ctatcccatc cagcgccttc ctgaatgcct
     2161 agaatatcag ggtctaactc tttgattact gacaatagtt ctggtaatcg gtcccttcca
     2221 tattgtgaat cttgtccttt ggctttcaaa ttctcgtcaa actcacggtc aacgcctgca
     2281 ccccaaagaa tgttatatga catcactttg accgttagta gtggctttgg cgttggagaa
     2341 accgtaggtg acggcaattc agttggtgtc tttgtaggta gatttgtaat tgtagatgga
     2401 acaccacaag atgttagaac aaagagaaag aatgccgaaa gaatgaaaag gcgtctcatt
     2461 ttagatttct ccaccgaata tgaaaatatt aacaaatact tgtttgtgcg accagccacc
     2521 caacggattg cgccatcgtc cccgtagggg gtacctgccc tgtctcgcgg accgggcgaa
     2581 gccgtccagc caacatgacc taaggcggtc tgggtttccc tttaaaggtg gttttgctct
     2641 tgactttcaa gacagttgct gcac
//
LOCUS       DAOREF010000421         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27119_length_2662_cov_3.946298, whole genome shotgun sequence.
ACCESSION   DAOREF010000421 DAOREF010000000
VERSION     DAOREF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27119_length_2662_cov_3.946298"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKI33_09300"
     CDS             <1..208
                     /locus_tag="PKI33_09300"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HNM37248.1"
                     /translation="DATDRAAIIMRYWYDYSEKEIAESLNLTVSAVKSRLHRARKELA
                     GLWAEQEDDLLAEMERRHHESPAF"
     gene            189..719
                     /locus_tag="PKI33_09305"
     CDS             189..719
                     /locus_tag="PKI33_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNM37249.1"
                     /translation="MNHQPFETWLLDDKVLTPTEKRELDSHLRTCKNCTALAETGLAL
                     RSSRVATPAPGFAMRFQQKLAAQKLAEQRRRLWGMIVLIVSGVGLLGWFLAPVLMSIT
                     TSPVGFLLSAAGLFAFLFTSIQAFGEVLTVMLRMLPAFLPPYMWMVLFSGAAGIGLLW
                     AVSIWRLVRRPQGVFA"
     gene            716..1753
                     /locus_tag="PKI33_09310"
     CDS             716..1753
                     /locus_tag="PKI33_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37250.1"
                     /translation="MKKSTLTRILLLLALLIVPTASIHAQTPNNDDVVLFGQNYTLEE
                     GETLNGSLAVFGGNIDIKEGAEVNGSIAIFGGNITFAKDTTINGDVAVFGGNVSVEGE
                     IAGDMVLFGGQALLESKSKVRNIATFGGQVTQQEGSIVTGEITNNAPPEVDTPPSLPE
                     EPTVDIQVNPLWNVLGKFGQALLIAVIGMLLTLFLRPQLDRTADTITRQPLMAGGYGL
                     IVFFALPIALVIMVITLILIPVAAIVVLLIPLAWLFGIVALGQEVGERFSKAINQVWA
                     PVLSTGLGTFLLMLVIGFLELIPCFGWIPSAAVTLISVGAAVMTWFGTRNPPGYTPAA
                     AAVVEEVPPAS"
     gene            complement(1810..>2662)
                     /locus_tag="PKI33_09315"
     CDS             complement(1810..>2662)
                     /locus_tag="PKI33_09315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37251.1"
                     /translation="GCLILAALGSSAFHPVAAVQATLRGREFLKGRETTATSLFFTAG
                     QMGHFVGPIITGLILAYLKIPGMYILPLVSIPIGFALMYQLKDNHPHPQPVKTVISRR
                     NRAGITFILVLMAVATMQSWAQANMVNLIPKYIKDLGQGATVYGSMAGLFMGGSALGN
                     MIGGHFADRYPKRFVASLALLFSAIPIFVISRIGWSGWLYALVPLAGAGTGAVHSIMV
                     VLAQRIIPGGMALASGLILGFIFSSGALGLLYTGHLAEVYGFPYVLVLTTGMVLVAAP
                     LALLLKE"
BASE COUNT          654 a          719 c          663 g          626 t
ORIGIN      
        1 ggatgccaca gaccgtgcgg cgatcattat gagatattgg tacgattatt cagaaaagga
       61 aattgccgaa tccttgaatc tgacggtaag cgcggtgaaa agccgcctgc accgtgcacg
      121 caaggaactg gctggcttgt gggctgaaca agaagatgac ctgctcgccg aaatggaaag
      181 gagacaccat gaatcaccag ccttttgaaa cctggctgtt ggacgataaa gtcctaaccc
      241 ctacagagaa acgtgaactc gactctcatc tgcgcacttg caagaattgc accgcactgg
      301 cagagaccgg gcttgcctta cgctcttctc gggtagcaac ccctgcacct gggtttgcca
      361 tgcgcttcca gcaaaaactg gcggcgcaaa agctggcaga acaacgccgc cgtctatggg
      421 gcatgatcgt tctcatcgtc agcggagttg ggttactcgg atggtttctt gctcccgttt
      481 tgatgtcgat cacaacctcg ccggtgggct ttttgctctc tgctgccggg ttatttgcat
      541 ttctattcac atccatacaa gcattcggtg aagtgctcac tgtaatgctg cgcatgcttc
      601 ctgcttttct ccctccctat atgtggatgg ttctcttctc tggagcagcg ggtattgggt
      661 tgttatgggc agtatccatc tggcgcctcg ttcgccgtcc tcaaggagtt tttgcatgaa
      721 aaaatcaacg ctaacccgaa tcctgcttct gctggcactt ttgatcgtgc ctacagcaag
      781 catccacgcg cagaccccga ataatgatga tgtcgttcta ttcggtcaaa actacacgct
      841 ggaagagggt gaaacgctga atggcagcct tgctgttttt ggcggcaata ttgatattaa
      901 agaaggggca gaggtcaacg ggtcgatcgc catctttggt ggaaacatta ccttcgccaa
      961 agataccacc atcaacggtg atgtcgccgt ctttggtgga aatgtcagcg ttgaaggtga
     1021 gatcgcaggc gatatggtgc tctttggcgg gcaagctttg cttgagtcaa agtcaaaagt
     1081 tcgcaacatc gccacctttg gcggacaagt cactcaacaa gagggctcta tcgtcactgg
     1141 tgagatcacc aacaatgcac cgcctgaagt ggatactccg cctagcctgc cagaagaacc
     1201 gacagtagat atacaggtca acccgttgtg gaatgtgctt ggaaaattcg gtcaggcttt
     1261 attgatcgcc gtcatcggca tgttgctcac gctcttcttg cgcccgcaat tagatcgcac
     1321 cgcagacacc atcacgcgtc aacccttaat ggctggcggc tacggcttga tcgtattctt
     1381 tgccctgccg attgcattgg tcattatggt cattacgtta atcttgatcc ccgttgcggc
     1441 aattgtcgtc ttgcttatcc ccttggcatg gctattcggc atcgtggcgt tgggtcagga
     1501 agttggtgaa cgctttagca aagccatcaa ccaagtttgg gcgccggttc tttccaccgg
     1561 tctcggaacc ttcttgctga tgttggtgat cggtttccta gaactcattc catgcttcgg
     1621 ttggattccc tcggcagctg tcaccctaat cagtgtgggt gccgccgtga tgacgtggtt
     1681 cggaacgcgt aacccaccgg gatacacccc cgccgcggcg gcggttgtgg aagaagttcc
     1741 ccccgcctct taattggact gtagcctgct acaaaccatc cgccttggtc aacacccaag
     1801 gcggattttt tattccttca ataataacgc caacggtgcc gccacaagga ccatacctgt
     1861 cgtcaacacc agcacatacg ggaagccata aacctccgcg agatgaccgg tatacagcaa
     1921 ccctaacgcc cccgaagaaa agataaagcc gagaatcaac cctgaagcca gagccatgcc
     1981 gccaggaatg atgcgttgtg ccaagaccac catgatgcta tgcacggcgc ccgtccctgc
     2041 ccctgcgagt ggaaccaaag catataacca cccagaccaa ccgattcggc taatcacaaa
     2101 gatcggaatg gcagagaaaa gtaaagccaa cgatgccaca aaccgcttgg gatatcggtc
     2161 tgcaaagtgc ccgccaatca tatttcctaa agccgagcca cccatgaaga gtcccgccat
     2221 gctgccatag accgtggcac cctgccccaa atctttgatg tattttggga tgaggttcac
     2281 catattcgcc tgtgcccaag attgcatggt agcaactgcc attaagacca gaataaatgt
     2341 gatgcctgcg cgattgcgtc tggaaatgac cgtcttgacc ggttgcgggt gtggatgatt
     2401 atctttcaac tggtacatca acgcaaagcc aatgggaatg gataccaaag gcaagatata
     2461 catgccagga attttcaaat atgccaaaat caacccagta ataataggac ctacaaaatg
     2521 tcccatttgc ccagcggtga aaaataatga agttgcggtt gtctctcgcc ctttcaagaa
     2581 ttcacgtccg cgcaaagtgg cttggacggc cgccacaggg tgaaacgcag aagaccccaa
     2641 cgccgcaagg ataagacaac cc
//
LOCUS       DAOREF010000422         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27143_length_2661_cov_3.459325, whole genome shotgun sequence.
ACCESSION   DAOREF010000422 DAOREF010000000
VERSION     DAOREF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27143_length_2661_cov_3.459325"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PKI33_09320"
     CDS             complement(<1..1285)
                     /locus_tag="PKI33_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM37252.1"
                     /translation="MNIYNKRSLFRRRDESSVYRMFFVVIFILGGIWLIRSVQQGDVK
                     PLFLPTPTPTRFATSYTMEGDANFTAGQLNAAIEAYQEAATIDPSNADVWAQLSRVQT
                     YSTALIVQREDLLKRLDEAIVSAEKAIEVNPDSSNAHAVYAFALDWKASYTSDSRERQ
                     TLLQDAEQHATRAILLDNTNVLAQAFYAEILVDQQKWTQAQQFIDQALAADPTLMDVH
                     RVHAYVLESLGEYALAIEAYDKAIAIAPNMTFLYLRAGAGYRRLAFESANDEVQRQLF
                     EKSLEYFAQTARINSQLGVKDPAPYISIAKTYSQMGEFFIAIRNIQKAIEFEPANAVL
                     YGELGVLYHKNRNYETGILALGCAINGCTGPESCDGRGGCGPNDVEAEVKPLALDGST
                     VYYYDILASELAALSTPKNNKCPDALDVAAIIENSE"
     gene            complement(1282..2538)
                     /locus_tag="PKI33_09325"
     CDS             complement(1282..2538)
                     /locus_tag="PKI33_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM37253.1"
                     /translation="MNKRRTRPNWFRLALLSLLVLGAAYVNRFVVTNVDPLGVPSPTP
                     TISAETLSDQGDELFKQGKLVPAIDAYNQAIVSQPDNPALHITLARTLAFAGKYKEAQ
                     TAAEDALLLNPNNSMAHAVRAWALNFQGQYAEAESSITRALELDPNNALAHAYYVEIL
                     VDSYYSGLGAFDGIEKAIEESKFAQALAPDLLETHRARGYILEATGNYEEAIREYDQA
                     ISINPNISDLYLATGRNYRILGIYDKAVDAFTRADALNPEDPNPDLLLSRTYATIGEY
                     GKAMQFAESAVANNPADTNLRGNYGVMLYRNAYWEDAIRELSYVVKGGLSEDGHEMEV
                     INLVPDAPRIAEYYFTYGLALARTDNCGEALQISQLIISRVPADELAVNNANEIVNNC
                     TENLNAPPATATEEPVATEVDATPTP"
BASE COUNT          708 a          650 c          599 g          704 t
ORIGIN      
        1 attcagagtt ttcgataatc gccgcaacat ctagtgcatc tgggcattta ttatttttcg
       61 gggtactcaa cgccgccaac tcagaggcaa gaatatcgta gtagtaaacc gtactgccat
      121 ccaacgccaa tggtttcact tcagcttcta catcgttagg accacagcca ccgcgtccat
      181 cacaagactc gggtcccgta caaccattaa tcgcgcaccc taaggcgaga ataccggtct
      241 catagttacg gttcttgtga tacagaacgc ctaactcgcc atacaaaacg gcattggcag
      301 gctcgaactc aattgctttt tgaatattac gaatggcgat gaagaactca cccatttgtg
      361 aataggtctt ggcaattgaa atataaggcg ccgggtcttt cacgcccaac tgagaattga
      421 tacgagccgt ctgtgcaaaa tattcaagcg acttctcaaa caactgtctt tgcacttcgt
      481 cattagcgct ttcaaaagca agacggcgat accctgcccc tgcccgcagg taaagaaatg
      541 tcatatttgg tgcaatcgca attgctttat catacgcttc aatcgcgagc gcatattcgc
      601 ccaaagattc caacacatag gcatgcacac ggtgcacatc catcaatgtg gggtcagctg
      661 ccaatgcctg atcgatgaac tgttgtgcct gtgtccattt ttgctgatcg acaaggattt
      721 ctgcatagaa agcctgtgcc aacacattcg tgttatcaag gaggatggca cgtgtagcgt
      781 gctgttctgc atcttgaagc aacgtctgtc tctcacggct atctgatgta taagaagctt
      841 tccaatctag tgcaaatgca tataccgcat gcgcattgct actatctggg ttcacctcaa
      901 tcgctttttc tgctgaaaca atcgcttcgt ccaaccgttt taacaagtct tcgcgttgaa
      961 caatcaatgc agtagagtat gtttgcacac gcgaaagctg tgcccaaaca tcagcattac
     1021 ttgggtcgat tgtggcagct tcttgataag cttcaatggc agcatttaac tgccctgccg
     1081 taaagtttgc atcgccttcc atggtgtagg aagtagcgaa acgggtggga gttggcgtgg
     1141 gcaaaaagag cggctttaca tcgccctgct gcaccgagcg gattagccaa attccgccga
     1201 gaatgaagat gacgacaaag aacatgcgat aaacactaga ctcatcgcgt cgcctaaata
     1261 aactgcgttt gttataaata ttcatggggt tggggtagca tctacttcag tagctacagg
     1321 ctcttctgtc gctgtagcag gaggagcatt caagttttcg gtgcaattat tgacgatttc
     1381 gttggcattg ttcacagcaa gttcatctgc tggaacacgc gaaatgatta actgcgaaat
     1441 ttgtaatgcc tcgccacagt tatctgtacg agcaagcgcc aaaccataag tgaaataata
     1501 ctcggcgata cgcggcgcat ctggcacaag gttgatcact tccatttcgt gaccatcttc
     1561 cgaaagtcca cccttgacca catatgaaag ttcacgaata gcatcttccc aataggcatt
     1621 acgatacagc atcacgccat aatttccgcg caagttggta tcggcagggt tattcgccac
     1681 tgctgattca gcgaattgca tggctttacc gtattcaccg atcgtggcat aggtgcggga
     1741 gagtaacagg tccggattgg ggtcttcagg gttcaatgca tcggcacgcg taaaagcatc
     1801 cactgccttg tcgtaaattc cgagaatacg ataatttctt cctgtagcaa ggtagaggtc
     1861 cgaaatattt gggttgatag aaatcgcctg atcatattca cggatagctt cttcgtaatt
     1921 tcccgtcgcc tccagaatat atccacgagc acgatgggtt tcaagcaaat caggcgcaag
     1981 tgcctgcgca aacttggatt cttcaatcgc tttctctatg ccatcaaagg cgcccaaacc
     2041 actgtaatat gaatcgacca aaatctctac ataataggca tgcgccaagg cattattagg
     2101 gtcaagctct aaagcacggg taatactaga ttctgcttca gcgtattgtc cttgaaaatt
     2161 cagtgcccac gcacgcacgg catgtgccat tgaattatta gggttgagta ataacgcatc
     2221 ttcggctgcg gtttgagctt ctttatattt gccagcaaat gccaacgtcc gtgccaaggt
     2281 gatgtgaagc gcagggttat ctggctgaga tacgatcgcc tggttatatg catcaatcgc
     2341 agggacaagt ttgccctgct tgaaaagttc atccccttga tcgctcaatg tttcggctga
     2401 aatagtcggc gttggagaag gtacgcctaa gggatcaaca ttcgtcacca caaaacggtt
     2461 gacataggct gcaccaagca ccaacaggct aagcaatgca aggcggaacc agtttgggcg
     2521 ggtccgcctt ttgttcatac tccacttact tcctcgtaaa tacataaaat catattacca
     2581 tccggcggaa agcaacgtca agcaggggtt ttcgcttcta atttgcttct tagcaaaaat
     2641 cacatttctg ccatgttttt g
//
LOCUS       DAOREF010000423         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27171_length_2659_cov_4.354455, whole genome shotgun sequence.
ACCESSION   DAOREF010000423 DAOREF010000000
VERSION     DAOREF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27171_length_2659_cov_4.354455"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKI33_09330"
     CDS             complement(<1..248)
                     /locus_tag="PKI33_09330"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNM37254.1"
                     /translation="MDPKILSQHFQTHFNAKPEIIVRAPGRVNLIGEHTDYNDGFVLP
                     MAIDREVMIALRPREDKTVRLFSLDLETESAFELSSLTR"
     gene            275..1831
                     /locus_tag="PKI33_09335"
     CDS             275..1831
                     /locus_tag="PKI33_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37255.1"
                     /translation="MDDLDLLKKYEPVLRFAKSERFFPMAVEPYLEKCALFPSGPSGA
                     AELFGHLNEPLIGKIGNLESHEFFLRFVNKPLNDFDAWIWWGVGSVLALVAGWFTLGM
                     AGVESVIGLSLSTALVLFMLASPIRLRIIPAMLAVLVFLVLGIAPIWLFFKPIPGVSI
                     AIEYLVLLPIYLLFLFYLLVRTLKFMIEKILPEGPGMVMDMLSQSTEKIAKEAARQYA
                     EIIREHRQPTYYGRVVRETDADGHSCVFLQYHFFYAFNDWRLAANGFNHHEGDWEMVA
                     VYLKDDEPHSVLLSQHGAGNIEAWETTLKALDKDGNETTHPVIYTALGSHANYSKPDV
                     IRSPAMYNPGSVQRFLFWFDGWIHYLFLLFNPNQKARQIALEEMRAKAHNLFEEHVFD
                     DLRDEEDHYVIRLPMEIATGDGLRVGFQGKNSREPMLKSSSYLKRVMSDRHVSLPPIR
                     EWRCILLNSEQGWVQYKGLWGVKSILSEESGPPGPRWDKPKRKKTGVSERVRWGKPLK
                     WLALLEKNKH"
     gene            1853..>2659
                     /locus_tag="PKI33_09340"
     CDS             1853..>2659
                     /locus_tag="PKI33_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37256.1"
                     /translation="MSISQALEVAIGLIFVYYVLGSIVSLVTQWINESFETRGKSVER
                     HLMKIVGDKHVDEFVKLPQLQALRPIRYKNMFSFLTSSTEPKKLEKIPAATLVDSYFD
                     FVGLTSQEFKADELKDFINAFPDSEGKQAMLKWVSQGVTNIEDLRKRTTTYFSGVMDQ
                     AASTFKSNARSFVIMLSIFLTLLLGTDSIQLAQTLWANASVRAIAVAQAQAVVQQEGS
                     NANVDDLLQQLLDLQIVKIGWWQTDLPQTTDIGTWGGFIALKFLGLGLTAV"
BASE COUNT          678 a          524 c          690 g          767 t
ORIGIN      
        1 cgggtgagag aagataactc gaaggctgat tcagtttcaa ggtccagaga aaagaggcga
       61 acggttttat cctcacgcgg gcggagtgcg atcatcactt cacggtcaat tgccatgggt
      121 aggacgaagc cgtcgttgta gtcggtatgc tcgccaatca agttgacccg cccaggtgcg
      181 cggacgatga tctccggttt ggcgttaaaa tgggtttgga aatgttgaga gagaattttc
      241 ggatccatat ctgcgtttat accatagagg cttcatggat gaccttgatt tactaaaaaa
      301 gtatgagcct gttttgcgct tcgcgaagag tgagcgtttc tttcccatgg ctgtagagcc
      361 ttatttggag aagtgtgcgc tcttcccaag cggaccaagc ggggcggcag agttattcgg
      421 tcatttgaac gaacccttga ttgggaaaat tggtaatttg gaaagccatg aattttttct
      481 gcgcttcgta aacaaaccgt tgaacgactt tgatgcatgg atctggtggg gagttggatc
      541 tgtgcttgcg ctggtggcgg gttggtttac attgggcatg gcaggtgtgg agagcgtgat
      601 tggcttatcg ctttcaacgg cattggtgtt gttcatgttg gcatcgccga tacgtttgcg
      661 catcatccct gccatgttgg cggttttggt ttttcttgtg ttgggcattg cacccatttg
      721 gttgtttttc aaacctattc caggtgtgag tattgctatt gagtatttag ttttgttgcc
      781 tatttatctt ttgttcttgt tctacttgtt agtacggact ttgaagttta tgatcgaaaa
      841 gattctgcct gaaggtccgg gcatggtgat ggatatgctt tcacaatcta cagagaagat
      901 tgcgaaagaa gccgccaggc aatatgcaga gataataaga gaacatcgtc agccaacgta
      961 ttatgggcgt gttgtgcgag aaacagatgc agatgggcat tcttgtgtat tcttgcaata
     1021 tcattttttt tatgctttca atgattggcg tttggcagcc aatggcttta atcatcacga
     1081 aggggattgg gagatggttg ctgtctatct taaagacgat gaaccccatt ctgttttatt
     1141 gtcgcaacat ggggctggaa atattgaagc atgggaaacc actcttaaag cattagacaa
     1201 agatggaaat gagacgactc accctgttat ttacaccgca ttgggatcac acgctaatta
     1261 cagcaaacca gatgtgatcc gttcgccggc aatgtataac ccggggagcg tgcaacgctt
     1321 cctcttctgg ttcgacggct ggatccacta tcttttcctt ttatttaacc cgaaccaaaa
     1381 agcacgtcag attgcattgg aggagatgcg tgctaaagct cataacttgt ttgaagagca
     1441 tgtcttcgac gatctgcggg atgaagaaga ccattatgtc atccgtttgc cgatggagat
     1501 tgccacaggc gatgggttac gagttggctt tcagggcaag aactcaagag agccgatgct
     1561 caagtcttcc agctatttga agcgagtgat gtctgaccgg catgtgtcgc tcccgccgat
     1621 tcgagaatgg cgatgtatcc tcttgaattc cgaacaagga tgggtacaat ataaaggatt
     1681 gtggggtgta aaaagcatac taagcgaaga gtcgggtccg cccggaccac gatgggataa
     1741 acccaaacgt aagaagacag gtgtaagtga gcgtgttcgt tggggcaagc cgctaaaatg
     1801 gcttgcgcta ctggaaaaga ataaacatta atgcaactca aaggagaaag atatgtcaat
     1861 ttcacaagcg cttgaagtag ccattggctt gatctttgtt tattacgtgt taggttcgat
     1921 cgtttctctc gtcacacaat ggatcaatga atcgttcgaa acacgtggta aatcagtgga
     1981 acgacatcta atgaagattg tcggcgataa acatgtggat gaatttgtaa aacttccgca
     2041 gttgcaggca ttacgcccta ttcgttataa gaatatgttt agcttcctta catcttctac
     2101 agaacctaaa aaactagaga agatccctgc tgcgactttg gttgattctt attttgattt
     2161 tgttgggctc acttctcaag aattcaaagc cgatgagtta aaagacttta ttaatgcttt
     2221 ccctgactca gaaggcaaac aggcgatgtt gaagtgggta agccagggtg tgaccaatat
     2281 tgaagacctg cgaaagcgta cgactaccta cttctctggt gtgatggatc aggcggcttc
     2341 aacctttaag tcaaacgctc gtagttttgt cattatgttg tctattttct tgaccttgtt
     2401 gcttggcaca gatagcattc agcttgcaca aaccttgtgg gcaaatgcga gtgtccgtgc
     2461 tattgcggtg gcacaggcac aagctgtggt gcaacaagaa ggcagcaatg ccaatgtgga
     2521 tgaccttctc caacaattgt tagatctaca aatcgtaaag atcggctggt ggcaaacaga
     2581 ccttcctcaa accacggata ttggcacatg gggcggattt attgctttga aatttctcgg
     2641 gcttggtttg accgctgtg
//
LOCUS       DAOREF010000424         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27173_length_2659_cov_3.923963, whole genome shotgun sequence.
ACCESSION   DAOREF010000424 DAOREF010000000
VERSION     DAOREF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27173_length_2659_cov_3.923963"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PKI33_09345"
     CDS             <1..1045
                     /locus_tag="PKI33_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37257.1"
                     /translation="AVIVMRGLKRSISIITIFGVVFLILGGSYFLWRWNYFGYPLPNP
                     FYKKGEGGWSWQTFDASMLNMLRLSLPVVLAFILGFRSNETLKTTLAYMIPIFGFAAA
                     FGLISDEMNYGARFQYATVPLMLMSWIPLVRGFKFDGLNQLQVRERAVYFVALFAVAA
                     GVVYYSWFQNCSLALYQQSCDRPYERDGRLEMARMLADYRGKGYVMAVTEAGLLPYYS
                     GWDAIDTWGLNDQFVAHNGSITVEYLDKYKPEIIMFHDYYSPLVPPKLTDANLAQRWF
                     SMTILLKTYAEENGYILAAVFGDSPYDTHYYYVRPGFEDSKRLVEQISKFRDYFYPTT
                     GKRSINYAMYAQP"
     gene            1063..1533
                     /locus_tag="PKI33_09350"
     CDS             1063..1533
                     /locus_tag="PKI33_09350"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM37258.1"
                     /translation="MSEIAELTEKLKTEGERMVAFFSELTDDQWVNEVYTEGTTWSIR
                     NVLSHFVTSERGLVKLFERIRATGEGASDDFSIDRYNASQQEKTKDLEPAELLEQYKQ
                     VRADSVQWVSGLKDEDLEIKGRHPFLGETVIREMIKMLYLHNQLHYRDVKRSLK"
     gene            1567..>2659
                     /locus_tag="PKI33_09355"
     CDS             1567..>2659
                     /locus_tag="PKI33_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM37259.1"
                     /translation="MELTPFKLERYFAKYEFNTEYLLCSSDCESMSVADLLALEAGAM
                     ERFHKHWLGYTESQGSPSLRKEIAGMYATTQAEDILVFTGANEAIYLFMMASLKENDH
                     VIVHSPHYQALSEIAKGIGCMVSPWRAREENGWALDLDELRHLMRSSTKAIIVNVPHN
                     PTGYLMPRADFDTLNQFAKENKLLLFSDEVYRESEYDSADRLPAAVDYGSHAVSLGVT
                     SKTYGLAGLRIGWVATKNRDLYAKIASLKDYTTICNSAPSEFLAEVAIRNRATLANRN
                     LEIIKNNLAIVDNLFSRHSSLFTWHRPRAGSMGFPKFLKGDVEAFCDDLVKKAGVLLL
                     PGSVYDDAHNHFRLGLGRKNLPQAAERLED"
BASE COUNT          647 a          534 c          670 g          808 t
ORIGIN      
        1 ggctgtcatc gtcatgcgtg gacttaagcg ttccatatcc attatcacaa tctttggggt
       61 tgttttcttg atattgggtg gttcatactt cctttggcgt tggaattatt ttggctatcc
      121 tcttccaaac ccattttata aaaagggtga aggtggttgg tcttggcaga cgtttgatgc
      181 gtctatgcta aatatgctga gattatcttt gcctgttgtg ttggcattta ttctcggctt
      241 tcgctcaaac gaaaccctta aaactacatt ggcttatatg attcccatct ttgggtttgc
      301 agcggcgttt ggcttgatct cagacgaaat gaattatggt gctcgttttc agtatgcaac
      361 cgtgccgctc atgctcatgt cgtggattcc gcttgtgcgc ggattcaagt ttgatgggtt
      421 gaaccagctt caggtccgcg aaagagccgt gtacttcgtc gcactgtttg ctgtagctgc
      481 tggagtggtc tactattctt ggttccaaaa ttgttctctg gctttatatc aacaatcgtg
      541 tgatcgccct tacgaacgtg atgggcgctt ggaaatggca agaatgttag cggactatcg
      601 tggaaagggt tatgtaatgg cggtgacaga ggcaggtttg ttgccgtatt attctggctg
      661 ggatgcaatt gatacatggg ggctgaacga tcaatttgtt gcacacaatg gttcgattac
      721 cgtggagtat ttggataaat acaagccgga gatcatcatg ttccatgatt actactcgcc
      781 gctggtgccg ccgaagttga cggatgctaa ccttgcgcaa cgctggttta gcatgacgat
      841 tttgttgaaa acctatgctg aagagaatgg ttatattctt gcggcagtgt ttggcgatag
      901 cccgtatgat acccattatt attatgtgcg ccccggcttt gaagatagca agcgtttagt
      961 ggagcagata tcaaagtttc gtgattattt ttacccgaca actgggaagc gctcgatcaa
     1021 ttatgcgatg tacgcccagc catagattta taggagatgc ccgtgtctga gattgctgaa
     1081 cttactgaaa agctcaaaac cgagggcgaa cgaatggttg ccttcttttc cgaattgaca
     1141 gatgaccagt gggtgaacga ggtttacacg gaagggacaa catggagcat tcgaaatgtg
     1201 ctttcgcatt tcgtcacttc tgaacgcgga ttggtgaagc tttttgaacg cattcgtgcc
     1261 acaggagaag gcgcttcgga tgatttctca atagaccgtt ataacgcttc tcaacaagaa
     1321 aagacaaaag atttagagcc tgctgagttg ctggagcaat acaagcaggt tcgcgctgac
     1381 tcggtgcaat gggtaagtgg gttgaaagat gaagatttgg aaattaaagg gcgacatcct
     1441 tttttagggg aaacggttat tcgtgaaatg ataaagatgc tttacttgca taatcaactt
     1501 cattatcgtg atgttaagcg ttcattgaaa tagctcaatg ctttttgtga atatctggag
     1561 actttcatgg aactgacccc gtttaagctt gaacgttatt ttgccaaata tgaatttaat
     1621 actgaatatt tactctgttc ttcagattgc gagtcgatgt ctgtagcaga tttgctcgct
     1681 ttagaagcag gtgctatgga gcgatttcat aagcattggt tggggtatac agagtctcag
     1741 gggagcccat cgttgcggaa agagattgct ggcatgtatg ccaccacgca ggcagaagac
     1801 atattagtct ttaccggggc aaatgaggcg atttacttat tcatgatggc ttcgttgaaa
     1861 gagaacgacc atgtaattgt ccattcgcca cattatcaag cgttgagtga aatcgccaaa
     1921 ggcatcggct gtatggttag cccgtggcgc gctcgcgaag agaatggctg ggcgttggat
     1981 ttggacgaat tacgccatct catgcgctca tcgaccaaag cgattatcgt aaatgttccc
     2041 cataatccaa ctggctatct tatgcctcgc gctgattttg ataccttaaa tcaatttgcc
     2101 aaggaaaata agcttctcct tttttcggat gaggtttatc gtgagtctga atacgattct
     2161 gcggatcgcc tccctgccgc cgtggattat ggatctcatg ctgtttctct tggagtgact
     2221 tctaaaactt acggcttggc aggtctgcgg attggttggg tcgccaccaa aaaccgtgat
     2281 ttgtacgcaa agattgcttc gttgaaggac tacaccacta tatgcaactc agctcccagc
     2341 gaatttctcg ccgaggttgc gatccggaat cgtgctactt tggcgaatcg aaatttagaa
     2401 atcatcaaga ataacctagc tattgttgat aatctatttt ctcgtcactc ttcgcttttt
     2461 acttggcatc gccccagggc tggttcgatg ggctttccca aatttctcaa aggggatgta
     2521 gaagcttttt gtgatgacct tgtcaaaaag gctggcgttc ttttgttgcc cggctcggtt
     2581 tatgatgatg cacataatca tttccgctta gggttgggaa gaaaaaatct tccgcaggcg
     2641 gcggaacggt tggaagatt
//
LOCUS       DAOREF010000425         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27214_length_2657_cov_3.278248, whole genome shotgun sequence.
ACCESSION   DAOREF010000425 DAOREF010000000
VERSION     DAOREF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27214_length_2657_cov_3.278248"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKI33_09360"
     CDS             <1..252
                     /locus_tag="PKI33_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNM37260.1"
                     /translation="YRLLDTLGTLIEIYEEEHYPIPDATGAEVLRFLMEEHGLTQSDL
                     SDVGSQGVVSEILNGKRELNVRQIRFLAEKFKVSSAVFL"
     gene            complement(681..2342)
                     /locus_tag="PKI33_09365"
     CDS             complement(681..2342)
                     /locus_tag="PKI33_09365"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM37261.1"
                     /translation="MKPNIFMSYSRRETGFVDDLTYRLEKAGFNVWLDFRRLIPGTPW
                     AGQIDKGLRESDVVLLVVSKDSIASQFVELEWRHVLEYEHKRIILAIFEAVKLPPELK
                     KYEWVDFRGSYEAGLKELIGQLNTPMQEMQPAPETGFKVPLIVWVTVIFAFFLALSSL
                     YSFWTILIPLILLPLPLQVLKRNYNFSRIQSILVFQPVSLFLVVLLMFETGVFQNEAI
                     LGLSDFYYSLSPFFRFLYDAMQWMFFLSIFLPFFLLRTAGMQRWGKPEANMPKFANPY
                     KPNNPKPKPITFFIDHVEQDSVMAKDISKGLTRYGHIPAADLKSAEAVFALVSRFKTD
                     TEADPEKQTVFPVMVQTAQPSEKLSHVQWVDFRKGLRNIDAVAQLLPDPAKMLTALGV
                     RPTSGTQTVMPGIISAFVNFLLGMVFLDIGSLAAYVIELWAFDGGAIMLPGILYFGMY
                     IIAMFLIFGVIYLVNTSLTARRGWLASINGLIFSQALLFVLFYWQAAQVDNIDALYAQ
                     SGVTDPGGYFIMVPFLVYIAGGFLLSAAALFRFSDIRRWFPAKTT"
     gene            complement(2424..>2657)
                     /locus_tag="PKI33_09370"
     CDS             complement(2424..>2657)
                     /locus_tag="PKI33_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37262.1"
                     /translation="PVKEISEFELSIGTLRVLSPTDCVKDRLCAFYFWNDLQGLEQAT
                     LVTKSQKVDLKEIKRWSKVEGKVKEFKAFTENL"
BASE COUNT          723 a          615 c          655 g          664 t
ORIGIN      
        1 tacaggttgt tggatacact cggaacgctt attgaaatct atgaagaaga gcattacccg
       61 atccctgatg cgactggtgc agaagtatta cgattcctaa tggaagaaca tggcttaact
      121 caatctgatt tatcagatgt gggatcgcaa ggtgtggttt ctgagatatt gaacggcaag
      181 cgcgaattga acgtccgcca gattcgattc cttgcagaga aattcaaggt ttcgagcgcg
      241 gtgtttttgt aatgtcaact ccaaacgggg gagggaaggg catgggcgtg tcttttatct
      301 tgtccgctgg cgcggacttt agctaagcaa gtcgcatttg cttaggcagt tttagcttct
      361 tcccttcagg ggagacggga gaggggtttg gtgtgccccc ctctgtcctt cggacatctc
      421 ccccaaattc catagtgcgg aatttagggg agactccgca ataggcattt ccttgcaact
      481 gggagaatat tcatccttca gcattcataa ttcatcattg ctcttttggc gggagaaggt
      541 attgagcgag ttgaggctga gggtggtcag atttcggaat gatgaggtga tgagggagtt
      601 gtccgcggtg gttgggagaa tcagaacgca agttgaattt gctttatagc acttccccag
      661 cataacaata tgctgccatc tcacgttgtc ttggctggga accagcgtcg aatgtcactg
      721 aagcggaaca gggctgctgc acttagaagg aaaccgcctg cgatatacac gaggaagggg
      781 accataatga agtacccgcc cggatccgtc acaccgcttt gggcataaag tgcgtctata
      841 ttatcaactt gtgctgcctg ccagtagaac aatacaaata acaacgcttg tgaaaaaatc
      901 aaaccgttta tcgaagccag ccagccgcgg cgcgcggtca acgaggtatt caccaggtag
      961 atcaccccaa agatgagaaa catggcgatg atatacatgc caaaatatag aatacccggc
     1021 aacataatgg cgccgccatc aaatgcccag agctcgatca cataggcggc taatgagcca
     1081 atatcgagaa aaaccatgcc cagtaaaaaa tttacgaagg cgctaatgat gcccggcatg
     1141 acggtctgtg taccgctggt ggggcgcacg cctaatgcag tcagcatctt tgcggggtcg
     1201 ggcagcaatt gagcaactgc atctatattg cgcaacccct tacgaaaatc gacccactgc
     1261 acgtgagaga gtttctctga aggttgagcg gtctgaacca taacggggaa aacggtctgt
     1321 ttttctggat cagcttccgt gtccgttttg aagcgtgaga ccaacgcgaa gactgcttca
     1381 gcggatttaa gatccgcagc ggggatgtgc ccatatctgg tcaatccctt tgagatatcc
     1441 ttcgccataa cagaatcctg ctcaacatga tcgataaaga aagtaattgg cttgggcttg
     1501 ggattgtttg gtttatacgg gttggcaaac tttggcatat tggcttcggg tttgccccag
     1561 cgttgcatcc ctgcagtgcg caacaggaag aacggcagga atatggacag gaaaaacatc
     1621 cattgcatcg catcgtacaa gaacctgaaa aatggggata aggaatagta aaaatcgctc
     1681 aaccccaata ttgcctcatt ttggaatacg cccgtttcga acattagcag gacgaccaaa
     1741 aatagtgaaa caggctgaaa caccaggatg ctctggatcc gcgagaaatt gtagttgcgt
     1801 ttgagtacct gcaagggaag cgggagcaag atcagcggga tcaagatcgt ccaaaacgaa
     1861 tatagcgaag aaagggcgag aaaaaaagca aagatcaccg tcacccaaac gatcaagggc
     1921 actttgaagc cggtttcagg tgcaggttgc atttcctgca ttggggtatt taattgaccg
     1981 atgagttcct taagccccgc ctcgtaactg ccgcgaaaat ccacccactc gtattttttt
     2041 aattcaggcg gaagttttac cgcttcgaaa atagcgagaa tgatgcgctt gtgctcatac
     2101 tccaatacat ggcgccattc caattccacg aactgcgacg cgatggaatc tttggaaacc
     2161 accaataaga ccacatctga ttcccttaat cccttgtcga tctgccctgc ccaaggtgtg
     2221 cctggaatta atcggcggaa atctaaccaa acattaaacc ctgccttttc caacctatag
     2281 gtcaggtcat ctacaaaacc agtttcgcgg cgtgaataac tcataaagat atttggtttc
     2341 atttaatgat cctttggcag gctgaatgta ctgcgttcaa atattaaacc acactgcaat
     2401 ccaatcgggt ggtgggggga agctcaaaga ttttctgtaa atgctttaaa ttcttttact
     2461 tttccctcta ccttcgacca acgtttgatt tctttcaagt ccactttttg gctttttgtc
     2521 accaaggttg cctgctccaa tccctgcaag tcattccaga aatagaacgc acagagcctg
     2581 tccttgacac agtctgtggg ggagagcact cgcaatgtcc ctatggagag ttcgaattca
     2641 ctgatctctt tcacagg
//
LOCUS       DAOREF010000426         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27419_length_2646_cov_4.508684, whole genome shotgun sequence.
ACCESSION   DAOREF010000426 DAOREF010000000
VERSION     DAOREF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27419_length_2646_cov_4.508684"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PKI33_09375"
     CDS             complement(<1..1563)
                     /locus_tag="PKI33_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM37263.1"
                     /translation="MFPDPFEDIQKQSQTLLTVYIWMFMSIMTAGCILLIVMDPSQLR
                     RPLLIIIILNSLYSLFYYIMKTGKVQLASFLTIASTWLMLTVVVTSGGGVHSPAFSAY
                     LMLTFLAGITLGVKPMMWTAIASLIVAGGMEYLELTGLLPEPRYQTSHFSFVLLQGIM
                     LFMFVVLQFYASSLIKNSFQKAQDELRERKIIEQALSASQRRFIAIFENSPIPTAITT
                     LNDLKLIDANSMFIQLYGYSLDELHGEQTRILGSLENQAQMLLLLNKNGFVRDFETSL
                     RRKDTETRYVSVTIGKLNIDASDCLIFQVLDVTEKHNLAQSLVESNQRFAQLTRHIHE
                     VFWVSEEVGKLEYVSPAFEYIFGFPAEDLYQGKDYIDLVHPEDRQIVLDSFEYDIRGE
                     NSLFEYRIIRPDGEIRWVSDRSYPILGEHGELIRTVGITTDITEQKHSEAERRELTSI
                     LEDAEQQVGLASWSQDILTGKSKWSPYMFTLFGFDPAEGIPSMPDYLERIHPEDRELL
                     GSALFKMMQGEET"
     gene            1798..2604
                     /locus_tag="PKI33_09380"
     CDS             1798..2604
                     /locus_tag="PKI33_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNM37264.1"
                     /translation="MIQSPLRYPGGKTRAVSLIKDFMPAHFDEYREPFIGGGSFFVFL
                     RQAYPEIKCWINDLNQELYFFWKQAQTDSENLAREIRKVRKQNKTGRSLFNEFTSIHV
                     DTLTAFERAVRFFVLNRITFSGLVEAGGYSQQAFDGRFTESAIERVAVLGKILQGVKI
                     TNWDYQKVLAEGSKSVFTFLDPPYLKATKSRLYGKNGILHTDFNHDTFARTMKACPHS
                     WLITYDDSPEIRKNFHFAHIHEWELQYGMNNYKQGKAEKGKELFIYNYKK"
BASE COUNT          719 a          540 c          630 g          757 t
ORIGIN      
        1 cgtttcttcg ccttgcatca tcttgaataa agcggagcca agcagttctc gatcttcggg
       61 gtggatgcgt tccaaataat ctggcatgct tggaatgccc tctgccgggt caaacccgaa
      121 cagtgtgaac atataggggc tccacttgct ttttcctgtc agaatatctt gagaccaact
      181 tgccagccca acttgttgtt cagcatcttc cagaatggat gttagttccc ggcgctcggc
      241 ttctgagtgt ttttgttctg tgatatcggt cgtgattcct actgtgcgaa tgagttcgcc
      301 atgttcccca aggatcgggt aggaacggtc tgagacccag cgaatttcac cgtctgggcg
      361 tatgatgcgg tattcgaata gcgagttctc gccgcgtatg tcatattcaa atgagtcgag
      421 cacgatctgg cggtcttccg ggtgtaccaa gtcgatgtaa tcttttcctt ggtacaagtc
      481 ttcggctggg aacccaaaaa tgtattcgaa cgccgggctg acgtattcca gtttgcctac
      541 ttcttcagag acccaaaaca cttcgtggat gtgccttgta agttgggcaa aacgttgatt
      601 gctttcgacc agagattgcg ctagattgtg tttttcggtg acatccaata cctgaaagat
      661 caggcagtct gatgcatcaa tattcagctt tccgatggtt accgacacat agcgggtctc
      721 agtatctttt cggcggagcg acgtttcaaa gtctcgtaca aagccattct tgttcaagag
      781 caaaagcatc tgcgcttgat tttcaaggct acccaggatt cttgtttgtt cgccatgcaa
      841 ttcatcgaga gaatagccat atagctgaat aaacatggag ttagcatcga tcagtttgag
      901 atcattcaat gttgtgatgg cagtgggaat gggactgttc tcgaatatgg caatgaagcg
      961 tctctggctg gcggaaagcg cctgttctat tattttgcgt tctcttaatt catcttgagc
     1021 tttttgaaaa gaattcttga taagtgaact ggcatagaat tgcaagacca caaacataaa
     1081 taacataatg ccttgcagca acacaaaact aaaatgggag gtttgatacc gcggctctgg
     1141 taaaagaccg gtcaattcga gatattccat tccgcctgca acgatgagac ttgcaatagc
     1201 agtccacatc atgggcttga ctcccagggt aatgcctgcg agaaaggtta gcatgaggta
     1261 agcactaaat gccggcgaat ggacgccacc tccgcttgtt acaaccacgg tgagcattag
     1321 ccaagtagat gcaatggtga ggaaactggc tagctgcacc ttgccggtct tcatgatgta
     1381 gtagaacagc gaatataagg aattgagaat gataatgatg agaagcggac gtcttagctg
     1441 agacgggtcc ataacgatca acaggatgca tcctgccgtc atgatgctca tgaacatcca
     1501 aatatagacg gtgagcaagg tctgggattg cttttgaatg tcttcgaagg ggtcggggaa
     1561 cacgaatagg tttcttaagg acttaagaaa atgagggggg gagttttggg gttctaccat
     1621 atgccaggga cctgcccgct tcgctgattt ctcaaacaaa agagatggac ttggttgtat
     1681 tttttcattt attccctgtg ccgtaaatac gtgaaatccc tacttgtctc aggcggatgg
     1741 ggatgttatg agggtgtaag gtggaataag gctgggttat actttcaaga ttcattcatg
     1801 attcaaagcc cccttcgtta ccccggcggt aaaacgcgag ctgtttccct tataaaagat
     1861 ttcatgcctg cccattttga tgagtaccgt gaacccttta taggcggcgg ttcatttttt
     1921 gttttcttac gacaagccta tccagaaatc aagtgctgga ttaatgattt aaaccaggaa
     1981 ctttattttt tttggaaaca agctcaaaca gattcggaaa atcttgctcg tgaaatccga
     2041 aaggttcgca agcaaaacaa aaccggacgc agccttttta atgaattcac atcaattcat
     2101 gttgatacat tgacagcgtt tgagagagcc gttcgttttt ttgttttgaa tcgaattacg
     2161 ttttctggtt tggtagaagc gggcggatat tctcaacaag cattcgatgg acgttttaca
     2221 gagtctgcta ttgagcgggt cgctgtatta gggaaaattt tgcaaggagt aaaaattaca
     2281 aattgggatt atcaaaaagt gttggcagaa ggaagtaaat cggtctttac ttttttagac
     2341 ccaccttatt tgaaagcgac aaagtcacgg ctttatggaa agaatggtat tttacatacc
     2401 gatttcaatc acgatacatt tgcaagaaca atgaaagctt gtccacattc atggttgatc
     2461 acatatgatg actcgcccga gatacgcaag aattttcact ttgcgcatat ccacgaatgg
     2521 gaattgcaat atggaatgaa taactacaaa caggggaagg cagaaaaagg aaaagaactc
     2581 tttatttaca attataaaaa gtgaccgccg ttcggcggtc acttttttgt tgttacttcc
     2641 taatac
//
LOCUS       DAOREF010000427         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27530_length_2640_cov_4.482012, whole genome shotgun sequence.
ACCESSION   DAOREF010000427 DAOREF010000000
VERSION     DAOREF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27530_length_2640_cov_4.482012"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKI33_09385"
     CDS             complement(<1..539)
                     /locus_tag="PKI33_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNM37265.1"
                     /translation="MSPNPREEEKLDPRVKRTRNLILTSFATLIAEKGFDSISVQDVA
                     NKAEINRVTFYAHFSDKYALLDYAIQKTFWTEIEKRSLNMCAYSADNLRNLIQVVCEF
                     LVKANSGCAKPSQQFESLAETTTKKQIFDLLSHWLNKPQLPTEIPATVATWAIYGLAT
                     HYSHMKKRPALEKFVDEALP"
     gene            676..1047
                     /locus_tag="PKI33_09390"
     CDS             676..1047
                     /locus_tag="PKI33_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNM37266.1"
                     /translation="MNIALWIVQGLLAVLYGMAGSMKTFKPDFYRNNPQTESWAKERS
                     DWGLRQIGIPEFLGALGLILPMVTGILPWLTPLAAVGLTVIQLLALFAVHLPKKEPIL
                     PNIILGALSVFIVVGRWGLFS"
     gene            1111..2142
                     /locus_tag="PKI33_09395"
     CDS             1111..2142
                     /locus_tag="PKI33_09395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNM37267.1"
                     /translation="MQNIEFGLDTFGDVPMDDSGKPVSYARAIRQVVDEAVLADSVGV
                     DVIALGEHHRVEYSISTPETVLAGIATKTNRIRLASGVTVLSSDDPVRVFQRFATLDA
                     LSNGRAEVILGRGSFTESFPLFGYDLRDYEVLFEEKIELFAELLKEKPVTWSGTKRAS
                     LENVDVFPKTESGHLSTWVGVGGSPESVIRTARYGLPLILAIIGGNPQRFAPYIDLYK
                     RAAAEFGTTPQAVGIHSPGFIADTDEEAWDLYYPHARVVRDRIGRERGWPPSNLQEYQ
                     RERETGSLYVGSPETVARKIARVVKALGVNRFDLIYTAGTQSISSRLRAVELFGTKVI
                     PMVREILAG"
     gene            2191..>2640
                     /locus_tag="PKI33_09400"
     CDS             2191..>2640
                     /locus_tag="PKI33_09400"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNM37268.1"
                     /translation="MKDNIYTMNIHTIGILGAGKVGTVLARLALSAGYRVLIGGSGDP
                     AKIALTIEVLTPGAVAVTAQEAASQADVVILALPLGKYRNIPVEALQGKLVIDAMNYW
                     WEVDGIRDDLTDPRSSSSEIVQAFLRESRVVKAFNHMGYHDLEEGALP"
BASE COUNT          550 a          615 c          778 g          697 t
ORIGIN      
        1 ggaagcgctt cgtctacgaa tttttcgagg gcggggcgtt ttttcatgtg gctgtagtgc
       61 gtggcaagcc cgtaaattgc ccaagtggcg acggtggcag ggatttcggt aggaagttgt
      121 ggtttgttga gccagtggga taacaagtca aagatctgtt tcttggtggt ggtttcagcc
      181 agcgactcga attgttggct cggtttggcg cagccgctgt ttgccttgac gagaaattcg
      241 catacgacct ggatcaggtt gcgcaaattg tcagcggagt aggcacacat attcaggctg
      301 cgcttttcaa tttcagtcca gaatgttttt tggatggcgt aatccagcag ggcgtattta
      361 tcggagaagt gggcgtagaa ggtgacgcgg ttgatctcgg ctttgttggc aacgtcctgc
      421 acggagatgg agtcgaatcc tttttcagcg atgagagtgg caaaggatgt cagaatcagg
      481 ttacgggtcc gtttgacgcg agggtccagt ttttcttctt ctctggggtt aggcgacatt
      541 ttcgttcctt tgtagtttaa gtaaacagtt tcggtttttt gtctgttgac tctgtgggcg
      601 aggttggtaa actaagctac atatgtagtt tatcttacat tcgtagttta tcaaaactca
      661 caaggagagt caagaatgaa tatcgcatta tggatcgtac aggggttgct agctgtgtta
      721 tatgggatgg cgggtagcat gaaaactttc aagcctgatt tctaccgcaa taatccgcag
      781 acggaatctt gggcaaagga acggagcgat tgggggcttc gccagatcgg tatccctgaa
      841 tttcttggcg cgctcgggct gattttgcca atggtcactg gcattctgcc gtggctgacg
      901 ccgcttgccg ctgttgggct tacggtcatt caattgcttg ccctgttcgc tgtgcatttg
      961 ccgaagaagg aaccgatttt gccgaatatt atattgggtg cgctgtcggt gtttattgtt
     1021 gttgggcgtt ggggactttt ctcctaagga aacaagggtt aatgtagtct acaataccag
     1081 caactattac ctcacatgga gaaccgcgtt atgcaaaata ttgaatttgg attggatacc
     1141 ttcggcgatg tgccgatgga cgactctggc aaaccggtct cgtatgcgag agcgatccgc
     1201 caggtggttg atgaggctgt gctggcggac tccgttggcg tggatgtgat cgcgctgggc
     1261 gaacatcatc gcgtggagta ttcgatctcg acgccagaga cagtgctggc gggcattgcc
     1321 acaaagacca atcggattcg gctcgcttcg ggcgtgaccg tgctgagttc cgatgaccct
     1381 gtgcgcgtgt tccagcgctt tgccacgctg gacgcgctct cgaacgggcg ggctgaagtc
     1441 atccttgggc gcggttcctt cactgagtct tttcccctct tcggttatga cctgcgcgac
     1501 tacgaagtgt tatttgaaga aaagatcgaa ctgtttgccg aactgctcaa agaaaaaccc
     1561 gttacgtgga gcggcaccaa acgtgcatcg cttgaaaacg tggatgtttt ccccaagacc
     1621 gagtcggggc atttgtccac ttgggtgggt gtcggtggtt caccagagtc ggtcattcgt
     1681 acagcgcgtt atgggcttcc gcttatcctt gccatcatcg gtggcaatcc gcaacgcttt
     1741 gccccttata ttgatctcta taaacgcgcc gcggcagagt tcggcacaac gccgcaagcc
     1801 gttgggattc actcacctgg tttcattgcc gacacggatg aagaggcgtg ggatttgtat
     1861 tacccccatg cgcgggtcgt tcgtgaccgc atcggcaggg agcgcggttg gcccccgtcc
     1921 aatttgcagg aatatcagcg tgaacgagaa acgggttcgc tctacgtcgg ttcgcccgaa
     1981 acggttgcgc gaaagatagc ccgtgttgtc aaagcgcttg gtgtgaaccg cttcgacctg
     2041 atctataccg caggtacaca gtccatcagt tcacgtttgc gtgcggtgga gttgtttggc
     2101 acgaaggtga ttccgatggt gcgcgagatt cttgcaggat aagcctgacc gctgaccgcg
     2161 gtcgaccgtt aacggtctgc cgtccaagat atgaaagaca atatctacac tatgaatatt
     2221 cacaccatcg gaattttagg cgctgggaaa gtaggcaccg tgcttgcgcg acttgctttg
     2281 tcggcgggat atcgcgtact gatcggcggt tcaggcgacc ctgcaaagat tgcgctgacg
     2341 atcgaagtcc tcacccctgg cgcggtggca gtcactgccc aggaagccgc gtcacaagcg
     2401 gatgtggtga tattggcact tccacttggt aaataccgca acatccctgt cgaagccttg
     2461 caaggcaaac ttgtcattga tgcaatgaat tactggtggg aagtggatgg tattcgcgac
     2521 gacctgaccg atccgcgtag ttcatccagc gagatcgtgc aggcgttcct gcgtgagtcg
     2581 cgtgtcgtca aggcgttcaa tcacatggga tatcatgatc ttgaagaggg cgcattaccc
//
LOCUS       DAOREF010000428         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27556_length_2639_cov_3.325464, whole genome shotgun sequence.
ACCESSION   DAOREF010000428 DAOREF010000000
VERSION     DAOREF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27556_length_2639_cov_3.325464"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKI33_09405"
     CDS             <1..346
                     /locus_tag="PKI33_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNM37269.1"
                     /translation="VSDEDAFPFGRDESEVRLGRAGRYAFGFYEKHPCYAPWSHTLID
                     FNGNVYVCCMTREKIPPLGNIIHQSFREIWEGAAYRQVRLKMHPPALKPCARCDDFID
                     QNKSIWETIGPY"
     gene            complement(343..951)
                     /locus_tag="PKI33_09410"
     CDS             complement(343..951)
                     /locus_tag="PKI33_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37270.1"
                     /translation="MNVLLLHGQGRTTNAMRLLGFRLGRLGHPVQYFRYYTRSETFEG
                     IVNRFVQWVNTVNDGQPYAIVGHSMGGLIARAGLPLLKNLPVHLFLLASPNRPPKLAP
                     YVQNNFLYRYLTTDCGQLVLNSNFYQKLPLPNIPATVIAGSAGPRVGWLPYGFEPNDG
                     ALSVQDASMGDDYELIQVPSIHTFIMNSKLTLHYIEQILNQS"
     gene            complement(957..1238)
                     /locus_tag="PKI33_09415"
     CDS             complement(957..1238)
                     /locus_tag="PKI33_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37271.1"
                     /translation="MAKCEMVVGYLVPHRCDNPALGTCVKCGRGFCDEHTNATKEGRV
                     CLACQQGLEMPVALPIAASTFTPADMVLFGSTSSFDDNDDNSDMFSDLS"
     gene            1250..2407
                     /locus_tag="PKI33_09420"
     CDS             1250..2407
                     /locus_tag="PKI33_09420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM37272.1"
                     /translation="MKIALLSEKYTPDLGGLAISTGRLGEMLAAAGHDVRLFAPTSNL
                     SPTIKQTQRSGGVYVTRFGAHKRVDDTLVDWFELIVEEHKREPFDLIHAYFLPMAGFV
                     GTYAGKYLSIPSVVSIRGNDIERAAFDPSKFSHVMYALQNASAVTTNASILAKKAKAF
                     FDREVHIIPNGIDTERFKPMEKNSVLAEALGLVDEKKKEEGKFVMGFVGELREKKGLA
                     TLLSGYAQATKTRPVSLLIVGEVREGEDKKYFEEFKSNNPQLSITVTGHVPHKDLPAY
                     YSLMDVFVHPSLRDGMPNAVLEAMACGVPVIATPVGGALDVLEDGVNGFLVDVNDAPQ
                     LAEKIAKTLKPLKELESVRRSARETVLHHCTFEEELQANLKIYANLGVTTK"
     gene            2404..>2639
                     /locus_tag="PKI33_09425"
     CDS             2404..>2639
                     /locus_tag="PKI33_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNM37273.1"
                     /translation="MKHRLEHDLLGEREIPNEVYYGVHTLRALENFPISKIPISNYPS
                     LINALACVKEACAKANLGLQLLEPEKANAVIKAC"
BASE COUNT          765 a          613 c          629 g          632 t
ORIGIN      
        1 ggtttcagat gaagatgctt ttccctttgg gcgagacgag tccgaggtcc ggttgggacg
       61 cgctggacgg tatgcatttg gcttctatga aaagcatccc tgctatgcac catggtcaca
      121 cacactgatc gatttcaatg gcaatgtcta tgtgtgttgt atgacacgcg aaaaaatccc
      181 tccattggga aacatcatcc atcaatcatt tagagaaatt tgggaaggcg ccgcctatcg
      241 ccaggtgcgc ttgaaaatgc atccgcctgc gctgaaacct tgcgctcgtt gcgatgattt
      301 tattgaccag aataaaagta tctgggaaac gatcggtcct tactaactct ggttgagaat
      361 ctgctcaata tagtgaagtg tcaactttga gttcataata aaggtatgaa tgctcggcac
      421 ttggatcaac tcataatcat cacccattga agcatcttgc acgctcaatg ccccatcatt
      481 gggctcgaag ccataaggta gccaaccgac acgtggtccg gcagagccgg cgatgaccgt
      541 tgcagggata ttcggtaatg gcaatttttg atagaagttc gagtttagaa ctaattgtcc
      601 gcaatctgta gtgagatacc tgtagagaaa attattttga acataaggtg ccaacttagg
      661 tggacggttc ggcgaagcca acaaaaacaa atgaactggc agattcttga gcaagggcaa
      721 cccagctcgg gcgatcaacc cgcccatcga atgccccacg atggcatagg gttgcccatc
      781 attcacagtg ttgacccact gcacaaaacg gtttacgatg ccttcaaatg tttcgctacg
      841 ggtgtaatat cgaaagtatt gcacaggatg cccaagcctg ccaagccgaa agccaagcag
      901 ccgcatggca ttggtcgtcc gcccttgccc gtgaagtaaa agaacgttca caaacgttag
      961 ctcaagtctg agaacatgtc gctgttatca tcattatcgt cgaaggaact ggtggagcca
     1021 aacaatacca tatccgccgg agtaaaggtc gaagcggcaa tcggtaaagc aaccggcatc
     1081 tcaagacctt gctggcaagc aagacaaaca cgcccttctt ttgttgcgtt cgtatgctcg
     1141 tcacaaaacc cgcgtccaca cttaacgcac gtccccaatg ctgggttatc acaacgatgc
     1201 ggaaccaaat aaccaacaac catttcacac tttgccatcg agacacctca tgaaaattgc
     1261 tttgcttagc gaaaagtata caccagacct tggcgggctc gcaatctcaa caggacgatt
     1321 gggggagatg cttgctgcgg ctggacatga cgtacgcctc ttcgccccga cttcaaacct
     1381 gtcacccacc atcaagcaga ctcaacgctc aggcggagtc tatgtcactc gcttcggcgc
     1441 acacaaacgc gtggacgata cattagtgga ttggttcgag ttgatcgtgg aagaacacaa
     1501 gcgcgagcca tttgatctga tccatgccta cttcctcccc atggcgggct ttgtcggtac
     1561 ctatgctgga aaatacctga gcattcccag tgtcgtctcc attcgtggaa atgacattga
     1621 acgcgccgcc ttcgacccaa gcaaattttc acatgtgatg tatgccttac aaaatgcaag
     1681 tgctgtaaca accaacgcca gcattttggc aaagaaagca aaagcctttt ttgaccgtga
     1741 ggtacatatc attccgaatg gaattgatac agaacggttt aaaccgatgg aaaaaaattc
     1801 agtcttagcc gaagcactgg gtttggtgga tgaaaagaag aaagaagaag gaaaatttgt
     1861 catggggttt gttggggaat taagagaaaa gaagggactt gcaaccctcc tttctggcta
     1921 cgcgcaagct acaaaaacca gaccggtttc tctgcttatt gttggcgaag tacgagaagg
     1981 cgaagataaa aagtattttg aagaattcaa aagcaacaat ccgcaactaa gcattactgt
     2041 tactggacat gtcccccaca aagacttacc tgcatattat tccttgatgg atgtctttgt
     2101 acatccgtcg ctgcgggatg ggatgccgaa tgccgttctc gaagccatgg catgcggagt
     2161 tccagtcatt gcaacaccag ttggtggagc gttagatgtg ttggaagatg gcgtgaatgg
     2221 atttctcgta gatgtaaatg atgcaccaca actagcagag aaaatagcta aaacgctaaa
     2281 accgcttaaa gaattagaat cagttcgtag atccgcacga gaaacggttc tacatcactg
     2341 tacgtttgag gaagagttgc aagcaaatct aaaaatttat gcaaatcttg gagtaacaac
     2401 aaaatgaagc atcgacttga gcacgacctt ttgggcgaac gtgagatccc gaatgaggtg
     2461 tattacgggg tgcatacgtt gcgtgcgctg gaaaatttcc cgatcagtaa aattccgatc
     2521 agcaattatc caagtctcat aaatgcattg gcatgtgtca aggaggcatg tgcaaaagca
     2581 aatttgggat tgcaattgct agagcccgaa aaagccaatg ccgtcattaa agcttgtga
//
LOCUS       DAOREF010000429         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27607_length_2636_cov_5.235955, whole genome shotgun sequence.
ACCESSION   DAOREF010000429 DAOREF010000000
VERSION     DAOREF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27607_length_2636_cov_5.235955"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKI33_09430"
     CDS             <1..381
                     /locus_tag="PKI33_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37274.1"
                     /translation="IIRDHEELGTYLASIIPAGSLVYWDGGNAYTPMIYVPEARIFPP
                     QINDGYTFHIGGDPDVLYYFSHWNAELDARWRAEADVFIIEAKRYANWKDFLTPQEFQ
                     EFAKPSVSPSCVEGAELRIFQRLP"
     gene            378..1334
                     /locus_tag="PKI33_09435"
     CDS             378..1334
                     /locus_tag="PKI33_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM37275.1"
                     /translation="MNLSLIIPVYNEQDNLPMLFEALYATMNSLGKTWEVILVDDGSR
                     DKSLSILEEYAAKDADHVRVISFRRNFGQTAAIAAGLDYSQGDIIILLDADMQNDPSD
                     IPMLLAKLDEGYDLVSGWRKQRKDNAITRNLPSMMANKLISMVTGVELHDYGCTLKAY
                     RRPVLEGFRLYGEMHRFIPVFASSVGARITEVVVNHHPRRFGKTKYGLERTVKVVLDL
                     FTVKFLVSFSSKPIYLFGGTGLGLMVISALTMLYLFIRRIFFLVGVTGSPLFQTSTML
                     FILGFQSMLMGLIAELLVRTYHESQRKTTYTVRTKININDND"
     gene            1327..2244
                     /locus_tag="PKI33_09440"
     CDS             1327..2244
                     /locus_tag="PKI33_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNM37276.1"
                     /translation="MIKWLRENRQMVARGLGSLLALALLLVLIQEEGDGELFSAIRRV
                     SLVYFLLALLALLISRTFAATRWFVLLRSAGVKIPLSRAIMLMFTGNFSSNFLPTTIG
                     GDVVRLGGAMQMGYDRAICLASLVVDRLIGMAGMALALPLGLIPLFSLNSSLVQSFTL
                     AGLFQKGVDFGKRTFEAFSIWLKKPLGLSGALLSTLGNQIFIYLMIYLLLQGIDHHVS
                     FWLVAGMYTLTYFVTLVPISINGLGVQELSMTFLLVQLGGLTSSESATIALLTRLLFI
                     LTSLPGAFFLPSILAAMNEKKVEIPESNG"
     gene            2251..>2636
                     /locus_tag="PKI33_09445"
     CDS             2251..>2636
                     /locus_tag="PKI33_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37277.1"
                     /translation="MESVSTRKFLQVWLWALAVVSLCAILQTIQRTTELEIVLLRSKW
                     IGLVGIFAFTAILSVWISFSSFLNHIVSWFDDLENQSLSPLIITFYIALIIFGFLVSI
                     FFRLYIFSNTLPQVMPILWIFLWASLA"
BASE COUNT          606 a          593 c          593 g          844 t
ORIGIN      
        1 atcatccgtg accatgagga gttgggaact taccttgctt cgatcattcc ggctgggagt
       61 ctggtatatt gggacggcgg caacgcctat accccgatga tctatgtgcc agaagcacgt
      121 attttcccgc cgcagatcaa tgatgggtat actttccaca ttggcggcga cccagatgtg
      181 ctgtactatt tcagccactg gaatgccgaa ttggatgccc gttggcgtgc cgaagcggat
      241 gttttcatca ttgaagccaa acgctacgca aattggaaag atttcttaac tcctcaggag
      301 ttccaagagt ttgcaaaacc ctctgtatct ccttcttgtg ttgaaggtgc agaactaagg
      361 atatttcaac gattaccatg aacctttctc tcattatccc tgtttataac gaacaggata
      421 acctcccaat gctttttgaa gctctttatg caacaatgaa ctcccttggc aagacgtggg
      481 aagttattct tgtagatgat ggcagccgtg ataaaagtct ttctattctt gaggaatacg
      541 ctgcaaaaga cgcagatcac gtccgtgtga tttcattccg tcgcaatttt gggcagacag
      601 ctgccattgc cgctggtttg gattattctc aaggtgacat catcattctg ttggatgcag
      661 atatgcagaa tgacccttct gatatcccca tgcttttagc aaaacttgat gaagggtatg
      721 acctcgtcag cggctggcgt aaacaacgta aagacaatgc cataacgcgt aacctcccct
      781 ctatgatggc gaataagctc atttctatgg ttacgggtgt ggagttgcat gattatggtt
      841 gcacgctaaa agcttatcgt cgcccggtct tggaaggttt tcgtttatac ggcgaaatgc
      901 atcgcttcat tccagtcttt gccagctcgg ttggagcacg aatcacagaa gtcgttgtca
      961 accaccaccc acgccgattt ggaaagacca agtatgggct ggaaagaacc gtgaaggtcg
     1021 ttctcgattt gtttacggtg aaattcttgg tcagtttttc atcaaaaccc atctacttgt
     1081 ttggtggaac tggcttgggg ttgatggtta ttagtgccct gactatgctg tacttgttca
     1141 ttcgccgtat tttcttcctt gttggtgtga ctggctcgcc gcttttccaa acaagcacca
     1201 tgttattcat tttaggtttc caatccatgt tgatgggttt gatcgcggag cttctggttc
     1261 gcacctatca cgagtcgcag cgtaagacga cgtatacagt tcgtacgaag atcaatatca
     1321 acgataatga ttaagtggtt gcgtgaaaat cgccagatgg ttgcccgtgg attaggcagc
     1381 cttctggcat tggctttact tttagtacta atccaagaag agggggatgg agaattattt
     1441 tcggcgattc gccgggtttc actagtatat tttttattgg cacttctcgc tttattaatc
     1501 tctcgcacat ttgcagcaac ccgttggttt gtcttgcttc gttcggctgg ggtaaagata
     1561 ccgttatcac gtgccattat gctcatgttc accggtaact tttcatccaa ttttttacca
     1621 acgacgattg gtggagatgt tgtgcgcttg ggcggcgcca tgcaaatggg ctatgaccgt
     1681 gcaatctgtc tcgcctcgct cgtagtggac cgtttgattg ggatggcagg aatggcattg
     1741 gcactccctt taggtttgat tcccctattt tctttgaata gtagtttggt tcagtctttt
     1801 acattggcag gtttatttca aaaaggggtg gattttggca aacgcacctt tgaagccttc
     1861 tccatctggt tgaagaaacc cttaggatta agcggggcat tattatccac acttggcaat
     1921 caaatattta tttatttgat gatctacctg ctcttgcaag gcattgacca tcacgtatct
     1981 ttctggcttg tagctggaat gtacaccctg acctatttcg ttaccctcgt accgatctct
     2041 attaatgggc ttggcgtgca ggaactttca atgacttttc tgcttgtcca acttggcgga
     2101 ttaacgtctt ctgaaagcgc gaccattgct ttgcttaccc gtctcttgtt catcctcact
     2161 agtctgcctg gggctttctt cttgccatcc attttggcgg cgatgaacga gaaaaaagtt
     2221 gaaataccag aatcgaacgg atgaagaaat atggaatccg tttctacgcg aaaatttctt
     2281 caagtttggt tatgggcgct ggcggttgtt agcctgtgtg caattttaca aaccattcaa
     2341 cgcacaactg aattagaaat tgttctgctc cgctccaagt ggattggctt ggtaggcatc
     2401 tttgcattta cagccatttt gtctgtatgg atatctttct cttccttttt gaaccatatt
     2461 gtttcttggt tcgatgacct cgaaaaccaa tccttatctc ccttaataat caccttttat
     2521 attgcactca tcattttcgg ctttcttgta agtatattct tccggctata tatttttagc
     2581 aataccctgc cccaagttat gcccatccta tggatattcc tatgggcaag ccttgc
//
LOCUS       DAOREF010000430         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27740_length_2629_cov_4.151515, whole genome shotgun sequence.
ACCESSION   DAOREF010000430 DAOREF010000000
VERSION     DAOREF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27740_length_2629_cov_4.151515"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKI33_09450"
     CDS             complement(<1..766)
                     /locus_tag="PKI33_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNM37278.1"
                     /translation="MSIPEDPNLPSFSPQDETPAPPPPVRRRRATRRSHIPTDAEGQA
                     ELIASLARRAYPSIELFVFSLVCGAILGLGYLLDSQALLLLGVLVAPLMTPWVGFLLS
                     ILSGSLRFMFETLMALLISIVFVFLGGLLTGLAALPFMPRTFDNVYLHSRLWIPELIV
                     FALGAIILVISFARSEDKPFLPSVLIAYSFYLPISAAGFGIGSGLPNVWLQGLLVFLV
                     HFAMVSIIGLITLFVLRLRPSAGGVVLSVVTLVIFGG"
     gene            877..1767
                     /locus_tag="PKI33_09455"
     CDS             877..1767
                     /locus_tag="PKI33_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37279.1"
                     /translation="MKEPRVYKTSPILLVFIGLLFLFFIGILLFAIGPNSVGFMIPVG
                     VFSLFLFGAAFISMASKTIISDDEIAVQGLLGTKSINWANVGRVSGWGYAMKLHSRDD
                     DVTLSISPRLPGYQEVIEFVGSKRPDLFSVQEYGSMKRGITQYLAVLLGLLMMGGVAV
                     AYIVAIMSNEQTITSSSFMPLLIIAVFALIMIVTTLSGPHSINLEGNSVNLKYLFSEK
                     SFQSNEIANVQLSFTQSRNGRHYFIALNLLSRKTIRISGLGMSLPIAYLVLKNWHLGR
                     STGSGAQTNIAPNWSDNTWR"
     gene            2107..>2629
                     /locus_tag="PKI33_09460"
     CDS             2107..>2629
                     /locus_tag="PKI33_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM37280.1"
                     /translation="MTQHRITDDLEALLDVLPANIRHAVEKANNSDKLLEIVIDLGRI
                     PTARFVDGEIALLNTEITRAEIDHITERIGTFDADNRAGMERTLHRISAIKNRRGAVV
                     GLTCRVGRAVYGTVDIIQDLIESGKSVLILGRPGVGKTTLLREAARILAENKRVVIVD
                     TSNEIGGDGDVPHP"
BASE COUNT          694 a          595 c          681 g          659 t
ORIGIN      
        1 ctccgccgaa gatgaccagc gttaccacgc tcaaaacaac tccacctgca gatggacgca
       61 gcctcaaaac aaagagcgta ataagaccaa tgatgctgac catggcaaag tggacaagaa
      121 acacaagcaa gccttgcaac cacacatttg gcagacctga gccaatgcca aaccctgcgg
      181 cgctgattgg taagtaaaac gaataagcaa ttagcacact tggtaagaaa ggtttatctt
      241 cagagcgtgc gaatgatatg acaaggatga ttgcgccgag ggcgaagacg attaattcag
      301 gaatccacag gcgcgagtgt aaatagacat tatcaaaagt acggggcatg aagggtagtg
      361 ctgccaaccc ggtaagcaag cccccaagaa aaacaaaaac gatgctgatg agcaatgcca
      421 tgagcgtttc gaacatgaaa cgcaaagacc cagaaagaat ggacagaaga aagcccaccc
      481 aaggcgtcat gagtggagca acgagaacgc ctagtaagag cagagcttga gagtcaagta
      541 agtagccaag cccaaggatt gcgccacata ccaaagagaa cacgaagagt tcaatggagg
      601 ggtaggcgcg gcgtgcaagc gacgcaatga gttctgcctg accttccgca tcggtaggga
      661 tgtgagaacg gcgcgtggca cgtctgcggc ggacgggtgg gggcggagcc ggggtttcat
      721 cctgcggcga gaaggacggc aggttggggt cttctgggat ggacatatgg caattataca
      781 ggaataacgg gtaggaaaga gaggtgatgg agtattacaa tatcttcatc gtatagttgg
      841 gaccttgtca ttcagaactg aaataggagc tttattatga aagaaccacg tgtgtataag
      901 acctcaccaa tccttttggt gttcatcggt cttttgtttt tattctttat tggaatacta
      961 cttttcgcaa tcggaccaaa ttccgttggg tttatgattc cagtaggtgt tttctctttg
     1021 tttttatttg gtgcggcatt tatctcaatg gcgtccaaga cgattatctc ggatgatgag
     1081 atcgccgtgc agggattgtt aggcacaaag agtattaatt gggcgaatgt tggcagggtt
     1141 tcgggctggg ggtatgccat gaaacttcat agtagagacg atgacgtgac cttatccatt
     1201 agcccgcgtt tgccggggta tcaagaggtg atcgagttcg tcggttcgaa gcgaccagac
     1261 ctttttagtg tgcaagaata tggcagtatg aaacgcggta ttacccaata tctggcggtc
     1321 ttattggggc ttcttatgat gggtggggtt gcggtcgcct acatagttgc gatcatgagt
     1381 aatgagcaga ctataacctc ctcttcattt atgccgcttt tgattattgc tgtgtttgca
     1441 ttgattatga ttgtcaccac cctctctgga ccgcattcta tcaatctaga aggaaactct
     1501 gtgaatttga agtacctgtt tagcgaaaaa agttttcagt ctaatgagat tgcaaatgtg
     1561 cagctttctt tcacacaaag ccgaaatggc aggcattatt tcattgcatt gaatttattg
     1621 agccggaaga ccattcgcat ttctggtctt ggtatgagtt tgccaattgc gtatctggtg
     1681 ctgaagaatt ggcatcttgg gcgttctacg ggttcaggtg ctcaaacgaa tattgccccg
     1741 aactggtctg acaatacgtg gcggtagggt agaactgttc agcatcgcag actagacaat
     1801 ttgcgatgct gaacggattt aattcgtcag acaaattgat gaaattaata tagattgcta
     1861 acaaaccatt aatcgcgtac ctgctataat agcctttgcc actacataac ggcacaggat
     1921 gcctgacacg cgaaactctg cgcgtgacgc cccaggaagg caggtacacc atcatgatcc
     1981 ttttcaactt ccccctcttt cacccttaag acaagccgcg tcttttgttt tgcgcgagcc
     2041 gatatattct tttccctttg acttttcacg ccacttgacc cttggcgttt ctggaaactt
     2101 aacattatga ctcaacatcg aatcacagac gacctcgaag ctctgttgga tgttctgcct
     2161 gcgaatatcc gccatgcggt tgagaaggca aataacagcg acaaactgct tgagatcgtc
     2221 attgaccttg ggcgcattcc aactgcccgc tttgtggacg gtgaaattgc gttgcttaat
     2281 actgagatca cccgcgccga gatcgaccac atcacggagc gtattggcac tttcgatgcc
     2341 gacaatcgcg ctggcatgga acgtaccctt catcgcatct ccgccatcaa aaatcgccgc
     2401 ggagccgtgg ttggtttaac ctgccgcgtc ggtcgcgccg tgtatggcac tgtggatatc
     2461 attcaagacc tgatcgaatc gggtaaatcc gttttgatct tgggtcgccc gggcgtgggc
     2521 aagaccacgc ttctgcgtga agccgcccgc atcctcgctg agaataagcg cgtagttatc
     2581 gtcgatacat cgaatgaaat cggcggcgat ggtgatgtgc cgcacccgg
//
LOCUS       DAOREF010000431         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27847_length_2622_cov_4.018699, whole genome shotgun sequence.
ACCESSION   DAOREF010000431 DAOREF010000000
VERSION     DAOREF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27847_length_2622_cov_4.018699"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKI33_09465"
     CDS             complement(<1..694)
                     /locus_tag="PKI33_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNM37281.1"
                     /translation="MRNAKIVATIGPASDSEQALESLIKAGMNVARLNFSHGTHEQHQ
                     VRVKRIREVSERLNMPVGILQDLQGPKIRVGKLDIPLQLAVNEEVCLYATNDTPPQNG
                     CQLIPVDFRELFESVQKGDKLLLDDGRLALQVTSAEGRQVHATVKVGGALSSHKGINL
                     PGVKLRIAGFTSKDKADLAFGISQGVDAVAISFVRSADDVREVRAAIKEFSAGKHEPL
                     LIAKLEKPEAMVE"
     gene            complement(904..2214)
                     /locus_tag="PKI33_09470"
     CDS             complement(904..2214)
                     /locus_tag="PKI33_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNM37282.1"
                     /translation="MNPSSIYQSRVTTAEEAIKQVKSGNRVFLTGNVSVPKVLLSALV
                     GHAPNINDVEVCQALTIGAADYVTEAMEGHLRVNSMFISANVRKAVQDGRADFTPVLL
                     SEFPLLFKRGILPVDVALIHVSRPDEHGFCSLGVEVGLTKSAAESAKIIIAEVNDQMP
                     RTLGDSFIHVSRLNYIVPVNYPIPEHKMAAEGDSDIVQGIAQHVASLIPDGATMQLGI
                     GSIPDAVLKFLMDKKDLGIHTELFSDGVIELVNAGVLTNARKSLHPGKIVAGFILGTQ
                     KLYDWVNDNPMVEMHPTEYVNDPFIIMQNERMVAVNSAIEVDLTGQVCADSIGPKLYS
                     GVGGQLDFIYGASRSKGGVPIIALPSTATMRDGTKVSKITAMLKPGAGVVTSRNHVHY
                     VVTEYGIADLYGKTIRQRAQALIKVANPIFHDELEKQAKELHYL"
     gene            complement(2211..>2622)
                     /locus_tag="PKI33_09475"
     CDS             complement(2211..>2622)
                     /locus_tag="PKI33_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37283.1"
                     /translation="ATAYADVVVGAVLVAGQRAPMVITREMVRAMKPRSVIIDVSIDQ
                     GGCVETSRPTMHDNPTFIEEGVVHYCVPNIASTVARTASHVFLNSAIPYITAIANTGI
                     DAAMKNDPSIEAAVNTHQGKLVNLVRLGGVEVKE"
BASE COUNT          679 a          684 c          614 g          645 t
ORIGIN      
        1 actcaaccat cgcttcaggc ttttctaact tggcgatcaa caacggttca tgtttaccgg
       61 ctgaaaattc cttaatcgcc gcgcggactt cacggacatc atcggcgctg cggacgaaag
      121 aaatcgcaac cgcatccacg ccttgggaga taccaaaagc aaggtccgct ttatcttttg
      181 aagtaaagcc tgcaatgcga agtttgacac cgggcaagtt gatgcctttg tgcgaagata
      241 atgccccacc aactttcaca gtagcatgaa cttgcctgcc ttctgccgaa gtgacttgca
      301 atgcgaggcg cccgtcatcc aaaagtagct tatcgccctt ctgtacagac tcgaacagct
      361 cacggaagtc cacagggatc aattggcagc cgttctgtgg cggagtatcg ttagtggcat
      421 acaagcaaac ctcttcattt accgcaagct gaagaggaat atccaattta cccacacgga
      481 tcttgggacc ctgcaaatct tgcaaaatgc caactggcat attaagccgt tctgatactt
      541 ctcgaattcg cttcacacga acttggtgct gttcatgggt gccgtgggag aagtttaacc
      601 gggcgacatt catgcccgcc ttgatcaagg attccaacgc ctgctctgaa tctgaggcgg
      661 gaccgatcgt tgctacaatt tttgcgttac gcatagtgct gcttcctgtc aataatgatt
      721 tgttctgcgt tgaaaataaa atccatcgca aacccgttaa aaaatacggg gtgtttgtgt
      781 ctcaaaacgc cccgtatgat aggtctaaaa cagaggaatg tcaagccggg gcaaattatt
      841 atgggtttaa caataaaaag ggcatggagt tacgcaaact ccatgccctt tttgggtact
      901 cttttaaagg taatgcagtt ctttcgcctg cttttccagc tcatcatgga aaatcgggtt
      961 cgccaccttg atcagtgcct gagcccgctg gcggatagtc ttgccgtata agtccgcgat
     1021 gccatactct gtcaccacat aatggacatg attacggctc gtgaccacgc cggcacctgg
     1081 ttttagcatg gcagtgatct tactcacctt ggtgccatct cgcatggttg cggtgctggg
     1141 caaagcaatg atgggcacac cacctttgga acgagacgcc ccgtaaatga aatctaactg
     1201 cccacccaca ccgctgtata gcttcggacc aatgctatcg gcacagactt gcccagtaag
     1261 gtccacttct atggcagagt tgaccgctac catgcgttca ttctgcataa tgataaacgg
     1321 atcattcaca tactccgtcg ggtgcatttc gaccattggg ttatcgttga cccagtcata
     1381 aagtttttgg gtacccaaga taaaaccagc cacaatcttg cccgggtgca gagatttgcg
     1441 agcattggtc aacactcctg cattgaccaa ttcgatcacg ccgtctgaga ataactcggt
     1501 atgaatcccg agatctttct tatccatcag aaatttcaac acagcatcag ggatggaacc
     1561 aatacccagt tgcatggtcg cgccatcggg aatcagcgaa gcaacatgct gggcaatgcc
     1621 ttggacgata tccgagtcac cttcggctgc catcttatgt tcagggatgg ggtaattgac
     1681 cggcacaatg tagttcaaac ggctcacatg aatgaaggaa tcacctaatg tacgcggcat
     1741 ctgatcattg acttcagcaa taatgatctt ggcagactca gccgcagact tggtaagccc
     1801 cacttccacg ccaaggctgc agaacccatg ttcatctggg cgagagacat gaatcagtgc
     1861 cacatccaca ggcagaatgc cacgtttgaa tagaagtggg aattctgaaa gtaatacagg
     1921 cgtaaagtct gcgcgaccat cttggaccgc tttacgaaca ttggcgctaa taaacataga
     1981 attgacgcgt aaatgtcctt ccatggcctc agttacgtaa tcggcagcgc caatcgtcaa
     2041 tgcctggcaa acttccacgt cattgatatt gggagcatgt cccaccagtg cagacaaaag
     2101 taccttgggt accgagacgt tcccggtcag aaagacgcgg ttgcccgatt tgacctgctt
     2161 gatggcttcc tctgcagtcg tcacacggga ttgataaatg gaagacggat tcattccttc
     2221 acctccacgc cacccaaacg tacaaggttg actaacttcc cttgatgtgt attgaccgca
     2281 gcttcaattg aagggtcatt cttcatggcg gcgtcaatgc ctgtgttcgc tattgctgtg
     2341 atgtagggaa tagctgagtt caaaaacaca tggctggctg tgcgcgccac tgtgctggca
     2401 atattgggaa cacagtaatg cacaacacct tcctcaatga aagtgggatt atcgtgcatg
     2461 gtcggacggg aggtttctac acaaccacct tgatcaatac tgacatcaat aatgaccgag
     2521 cgcggtttca tagcacggac catttcgcgt gtgatcacca tcggtgcacg ttgacctgcc
     2581 accaaaaccg cccccacaac tacgtccgca taggctgtgg ca
//
LOCUS       DAOREF010000432         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27956_length_2616_cov_4.934401, whole genome shotgun sequence.
ACCESSION   DAOREF010000432 DAOREF010000000
VERSION     DAOREF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27956_length_2616_cov_4.934401"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="secD"
                     /locus_tag="PKI33_09480"
     CDS             <1..931
                     /gene="secD"
                     /locus_tag="PKI33_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNM37284.1"
                     /translation="FAAGDTTAAATPAPDGTTPVIYHTVMTGTSLDTASVVRSQTGEY
                     EISFTLKADAASVFGEYTTTHTNQYLAIVLDKVVISTPVIRQPITSGQGVISGNFTSE
                     SAQTLAIQLRSGALPIPIKVVESRTVGPTLGEESVRKSVLAGLIGLTVVVLFMGFYYR
                     LPGVIADLALICYAIFSLMLFKLIPVVLTLPGIAGFILSVGMAVDANVLIFERLKEEL
                     RAGRNLRHAIDLAWSRAWSSIRDSNTSTLITCVILFIFGSTFGASMVKGFAVTLAIGV
                     LVSLFTAITVTRTFLHAILDNVKNTEHPSWFGL"
     gene            941..1885
                     /gene="secF"
                     /locus_tag="PKI33_09485"
     CDS             941..1885
                     /gene="secF"
                     /locus_tag="PKI33_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNM37285.1"
                     /translation="MNIIKNRYLYFLISLLVIIPGLVFMGLNWANTKQGPLVLGIDFR
                     GGSLLEIQFEGTRPSSSDIAALYAEFSTEEENFKDPVIQPLGEDAYSIRSKTMTDETK
                     GKLVAEMESRFGSKITVLNFTSVSASVGAEVTRAAGVAIFMAAAAILIYIWWAFRSVE
                     HAYRYGTAAIIAMLHDVFVVLGAEAILGYFFGWEADALFLTALLTVIGFSVHDTIVVF
                     DRVRENSNLGRKIGYESMINHSIVQTLDRSLTTQLTVMFTLFALSLFGGDSIRHFTII
                     LLVGILSGTYSSIFNAAPILVVWENREWTTWFGRKASA"
     gene            2002..2601
                     /locus_tag="PKI33_09490"
     CDS             2002..2601
                     /locus_tag="PKI33_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNM37286.1"
                     /translation="MMLFLRAFIIGISIAAPVGPIGVLCIRRTLANGRLAGFVSGMGA
                     ASADMVYGAIAAFGLTAITGLLVDNAFWFRIVGGLFLLYLGIKTLLEKPSDKPAQAAQ
                     GNLLSMYTSTFLLTITNPMTILSFAAIFAGTMLGKTTGSPLVMVAGVFAGSAAWWLTL
                     SFIVGILRDRLTQVHMAWINRISGAIIIAFGVYALFSGS"
BASE COUNT          564 a          649 c          616 g          787 t
ORIGIN      
        1 cttcgcggca ggcgatacaa cggctgctgc tacccccgcc cccgatggca cgacacctgt
       61 gatctatcac accgtgatga ccggtaccag cttggataca gcaagtgttg ttcgcagcca
      121 gaccggtgaa tatgaaattt cattcacgtt gaaggctgat gccgctagtg tctttggtga
      181 atacaccaca acccatacaa atcaatatct tgcaatcgtg ctggataagg ttgtgatttc
      241 tacccctgta attcgccaac ccatcacaag cggacagggc gttatctctg ggaacttcac
      301 ttccgaatct gcccagaccc tcgccattca gcttcgttct ggtgccttgc ccattccgat
      361 caaggttgtt gaaagccgca cagttggtcc gaccctcggt gaggaaagtg tgcgtaagag
      421 cgtgttggca ggtctcattg gtttgaccgt tgtggttctc ttcatgggct tttactatcg
      481 cctccccggc gtcattgcag accttgcgtt gatttgttac gccattttct ctttgatgct
      541 tttcaagttg atccccgtgg tgttgacctt gcccggtatc gctggcttta ttctttctgt
      601 gggtatggca gtagatgcca acgtgttgat ctttgaacgc ttgaaggaag aattacgcgc
      661 tggacgcaat ctgcgtcacg ccatcgatct tgcttggagc cgtgcttggt cttccattcg
      721 agactccaac acatcgacac tcatcacatg tgtcatcttg ttcattttcg gcagcacctt
      781 tggcgcaagc atggtaaaag gttttgcagt taccttggcg attggcgtgc tcgtcagcct
      841 cttcactgcc atcaccgtaa cacgcacctt cctgcatgcc attctagaca atgttaagaa
      901 taccgaacac cccagctggt ttgggcttta ggacaatacc atgaacataa tcaagaatcg
      961 ctatctatac tttctgatct ccctgcttgt gattatccca gggttggtct tcatgggact
     1021 aaattgggca aacacgaaac agggtccgtt agttcttgga attgatttcc gaggcggctc
     1081 cttattagag atccagtttg aaggcactcg cccctcatct tcagacattg ctgctcttta
     1141 cgccgaattc tctacagaag aagaaaactt caaagacccc gttattcaac cgctcggtga
     1201 agatgcctat tccattcgct caaagaccat gaccgatgaa accaaaggca agcttgttgc
     1261 tgaaatggaa tctcgttttg ggtccaaaat cactgtgttg aacttcacct ctgttagcgc
     1321 ctctgttggt gctgaggtga ctcgtgctgc aggtgttgct attttcatgg cagcggctgc
     1381 gatcctcatc tatatttggt gggcgttccg aagtgtggaa cacgcctatc gctacggcac
     1441 agcggcaatc atcgccatgt tgcatgatgt attcgttgtg cttggtgccg aagccatttt
     1501 gggctatttc ttcggctggg aagcagatgc gctcttcctc actgcgctcc tcaccgtaat
     1561 cggtttttca gttcacgaca caattgtggt gttcgacaga gttcgcgaaa attccaatct
     1621 tggtcgcaaa attggttatg aatccatgat caatcactcc attgtccaaa ccttggaccg
     1681 ctctctgacc acccagttga ccgttatgtt cacgcttttc gccctctcgc tttttggtgg
     1741 cgatagcatt cggcatttca ccatcatctt gttggtaggt atcttaagcg gtacctactc
     1801 atccattttc aatgcggcgc ccattcttgt cgtttgggaa aatcgcgaat ggacaacctg
     1861 gttcggtcga aaagcaagcg cgtaaaatac gcttcaaaac agccatcagg tttgatggct
     1921 gtttttttta tttcaaagga tgatttctta ctttattaaa attagtaatt agacgcatat
     1981 cgcgtcgctt gcaggtgatt catgatgctt tttcttcgtg cttttattat cggaatttca
     2041 attgctgccc cggtcggacc cattggcgtg ttatgcatcc gccgcacatt agcgaacggt
     2101 aggttggcag gttttgtttc tggcatgggt gctgccagtg cagatatggt ttacggtgcc
     2161 attgccgctt ttggattaac tgcaattacg ggattactgg tagataacgc cttctggttt
     2221 cgtatcgttg gtgggttgtt tttgctttat ctaggcataa agacattgct cgagaagcct
     2281 tctgacaagc ctgctcaagc agcgcaaggc aacctgctca gtatgtacac atctacattt
     2341 ctgctgacga tcacaaaccc tatgactatt ctctcgtttg cagcgatttt tgctggaacc
     2401 atgctcggaa agacgaccgg gtctcctttg gtcatggttg caggcgtatt tgctgggtcc
     2461 gctgcttggt ggctgaccct tagttttatt gttggcatac tacgagatcg gctgacccaa
     2521 gttcatatgg cgtggattaa ccgaatttca ggtgcaatca tcattgcttt tggagtgtac
     2581 gccttattta gcggaagcta aataaaaagt gccagc
//
LOCUS       DAOREF010000433         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_27960_length_2616_cov_3.186255, whole genome shotgun sequence.
ACCESSION   DAOREF010000433 DAOREF010000000
VERSION     DAOREF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27960_length_2616_cov_3.186255"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKI33_09495"
     CDS             complement(<1..458)
                     /locus_tag="PKI33_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNM37287.1"
                     /translation="MNIARLNFAHLPTPIEELPNLSKALGGPRIFVKRDDQTGLAFGG
                     NKTRKLEFLIAEAREQGADLLISGGAMQSNHCRQTAAAAARYGFECKLVLTGVKPQQA
                     TANYLLDQLFGAEIITVEDRAFRDEILKKTFDDAVAAGKKPYLVPYGGSSP"
     gene            complement(455..1015)
                     /locus_tag="PKI33_09500"
     CDS             complement(455..1015)
                     /locus_tag="PKI33_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37288.1"
                     /translation="MESSSPAPVLDPSNTTNIKFIILGIVIAFIYGFSTFLLVFIFLP
                     VFFIFLSYFIGDQQDLVIVLTPLISTLSGGLAAFLSGRAAFRSANKSGSIWNVVIGTI
                     VSVVAGIFGVLFFSVLIYFIGGLIYGNYYEWSAGAGIALIATAFTLGCALAYGTAVIA
                     GALTHSMLARRSIVLRNNAKIEEGLS"
     gene            complement(1075..2220)
                     /locus_tag="PKI33_09505"
     CDS             complement(1075..2220)
                     /locus_tag="PKI33_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNM37289.1"
                     /translation="MNSNFWNQIISNLPDPHFLQSYEWGQVKAKYGWAPYYAVWSADG
                     KFTVSQTTDNWSLDTSNFVAASLILKRTALRRFSIFYAPKGPLMDWTNESLRKRVLDD
                     LQFFAKRQGAIFLKFDPDVVLGRGVPASVGEVTENSGQAVMSDLKRRGWVESSDQIQF
                     KNTVMVDLSASEEDILMRMKQKTRYNVRLAEKKGVVIRVGNVEDLPALYKMYAETSVR
                     DGFVIRNEEYYMTVWKLFMQGAVSGQPSAVPLIAEVDGEAVAAIFLFMFAGRGYYVYG
                     MSRDKHREKMPTYLLQWVAMKHAKAYGCLTYDLWGAPDVFDESDSMWGVYRFKEGLGG
                     EVVRTLGAYDFAPSKFWYSMYSDVMPRVLNFMRSKGRESTRRQLSGE"
     gene            complement(2223..>2616)
                     /locus_tag="PKI33_09510"
     CDS             complement(2223..>2616)
                     /locus_tag="PKI33_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNM37290.1"
                     /translation="HPKGTCELLVAEYEGKPLASLMVFANAKRALYVYGASNNEERNR
                     MPTYLLQWEAIRWAKARGCEEYDLWGVPDENEETLEAQFESRHDGLWGVYRFKRGFGG
                     QLKRAVQALDRVYNPLLYWAYLKFIGNR"
BASE COUNT          727 a          711 c          571 g          607 t
ORIGIN      
        1 gggctggaac cgccatacgg aacgagatag ggtttcttcc ctgcagcaac tgcatcgtca
       61 aatgtcttct ttagtatttc gtcacggaag gcacggtctt cgacggtgat gatctccgcg
      121 ccgaagagtt ggtcaagcaa ataattagca gtggcttgct gcggcttgac cccagtcaac
      181 accaacttgc attcaaagcc atagcgcgcc gcagcggcag ctgtctggcg gcaatgattg
      241 gactgcatcg ccccaccgga gatgagcaag tccgcgcctt gttcgcgtgc ttcagcaatg
      301 agaaattcca gcttgcgggt cttgttaccg ccaaacgcca acccggtctg atcatcgcgc
      361 ttgacgaaga tgcgcggtcc gccaagggct ttggataaat tgggcagttc ttcaattggt
      421 gtgggaagat gggcaaagtt taagcgggca atattcatga aagaccttct tcaatcttgg
      481 cgttattcct taatacaatt gaacgtcgtg cgagcatgga atgagtcaat gccccggcaa
      541 taacagccgt accatatgca agagcacaac ccaaagtgaa ggctgttgct atcaacgcga
      601 tccctgctcc ggcgctccac tcgtaataat taccgtatat taagccgcca ataaagtaaa
      661 tcaaaacaga aaaaaacaac acaccgaaga tgcccgcaac aacacttaca attgttccga
      721 tcactacatt ccaaatactg ccgcttttat ttgcgcttcg aaaagcagct ctaccgctca
      781 agaatgcagc caaaccacct gacagggtgg aaatcaacgg cgttaaaaca atgactaaat
      841 cctgctgatc gccgataaaa tagcttaaaa atataaaaaa tacaggcaaa aaaataaaga
      901 caagaaggaa cgtcgaaaat ccatatataa acgcaatcac aatccccaaa ataatgaatt
      961 ttatattagt cgtgttagac ggatctaaaa caggagcagg ggaagaactt tccataggga
     1021 tacctccaac atataaattg aaaacaaagt tgattttact tgtaaatcaa tggtttactc
     1081 cccacttaat tggcggcgcg tactctctct tcctttcgat ctcataaaat tcaacactcg
     1141 cggcatcacg tccgagtaca tcgagtacca gaacttgctg ggcgcaaagt catacgcacc
     1201 gagcgtccgc accacctcgc cacccaagcc ttctttgaag cgatacacac cccacatcga
     1261 gtcgctttca tcgaagacat ccggcgcacc ccacaggtca taagtcaagc aaccgtacgc
     1321 cttggcatgt ttcatcgcca cccactgcaa aagataggtc ggcatttttt cacgatgctt
     1381 atcccgcgac atgccgtaga cgtaatatcc gcgcccggcg aacataaaca aaaatatcgc
     1441 cgccactgct tccccatcta cttcggcaat caaagggacc gcggacggct gaccgctgac
     1501 cgcgccttgc ataaataact tccaaaccgt catgtaatat tcttcattgc ggatcacaaa
     1561 gccatcacgc accgatgttt cggcatacat cttgtacaag gcaggcaaat cttctacatt
     1621 gcctacgcga atgacgacac ccttcttctc agcaaggcgc acgttgtaac gcgtcttctg
     1681 tttcatgcgc atgaggatat cttcttcgct ggcagataga tcgaccatga ccgtattttt
     1741 gaactggatc tggtcagacg actcgaccca gcccctgcgc ttcaaatcgg acatcacagc
     1801 ctgtccgctg ttttctgtga cctcacccac gctggcaggg actccgcgcc ccagcactac
     1861 atccgggtca aatttcagga agatcgcacc ttgtctcttt gcgaaaaatt gcagatcgtc
     1921 caacacacgc tttcgaaggg actcattcgt ccaatccatg agtggacctt tgggggcgta
     1981 gaaaatggag aagcggcgga gagcggtgcg tttcaggatg agggatgcgg caacaaagtt
     2041 actagtatcc agtgaccagt tatcagtggt ttgagaaacg gtaaattttc cgtcagcaga
     2101 ccagacggcg taatagggag cccagccata cttcgccttc acttgtcccc attcgtaaga
     2161 ttgcaaaaag tgtgggtcag gtaaattgga gattatttga ttccagaaat tactattcac
     2221 tattacctat tcccaataaa cttcaaataa gcccaataca acagcggatt gtaaactcta
     2281 tccaacgcct gtaccgcgcg tttgagttga ccaccaaatc cgcgcttgaa gcgatagaca
     2341 ccccacaagc catcgtggcg agattcgaat tgggcctcga gcgtctcttc gttttcgtca
     2401 ggaacacccc acaggtcata ttcttcacat ccacgcgcct ttgcccagcg gatggcttcc
     2461 cattgcagaa gataggtcgg catgcggttg cgctcttcat tgttcgacgc accataaaca
     2521 tacaaagcac gtttggcatt ggcaaaaacc atcaacgaag ccagcggttt gccttcgtat
     2581 tcagccacga gcaattcaca cgtccctttc gggtgg
//
LOCUS       DAOREF010000434         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28017_length_2613_cov_4.623925, whole genome shotgun sequence.
ACCESSION   DAOREF010000434 DAOREF010000000
VERSION     DAOREF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28017_length_2613_cov_4.623925"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKI33_09515"
     CDS             <1..404
                     /locus_tag="PKI33_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM37291.1"
                     /translation="DIFMMWSDGAGPTQPWSRIRHLISSEFVGDAVNWNGNWGQYSNP
                     AADELINAIPNESDPAKLNEMYTELVKIYLTDVPSFTLMYRPQAFHTVNESVWTNFPH
                     GEDGTNPPVPPLDLTDGWSIAGLYNLTLVNP"
     gene            674..1681
                     /locus_tag="PKI33_09520"
     CDS             674..1681
                     /locus_tag="PKI33_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37292.1"
                     /translation="MLKGYLKYFLNKLGWFAITFLFAFILNFLLPRLMPGDPVAAIVS
                     RMAQGMSNTSGVQAIYEQYTELFGTNKPMIEQFLIYVKNVARGDFGFSFSQYPRTVSD
                     VIGASIWWTLALQFPAIIVGWIIGNTLGALAAYLKGGFDKILMPISIFVSNFPAFGMA
                     VIFMVIFGVNLKWFPTSGGYGFDMIPNFSWAFIKSVIIHYQLPFWSIVVIAIGGQAIG
                     MRSMSIYELNADYVKYARFMGIRDSKIVGYVFRNAMLPQVTGLALSVGTMVGGALVAE
                     IVFSYPGLGTTLLRAVLGQDYPLISASTLIITVMALFANFIIEILYGLIDPRIKAAQS
                     D"
     gene            1696..>2613
                     /locus_tag="PKI33_09525"
     CDS             1696..>2613
                     /locus_tag="PKI33_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37293.1"
                     /translation="MKHIIQQVFRSGKFVTGFSIFSAIILIVIFYPLIITDQPLAIIG
                     QGTFFPPGIYVSVYDSMSANAYTLNLDEAAEMRIASKLNDEDRLAVKEWLVAAGIPES
                     DIDITDTAKLLDQWFANYDSKKNVPGMTNAKRNYYIRLNNALKGLLSAEAATVMQTSP
                     QTNELEQIDTIQQTDYVNVGEVANVRVLPLGTDNFGRDVLTELVSATRVSLQIGLVAG
                     IIATIIGLTLGLLAGYIGGLVDDLIMFVTNLFIVIPPFVLLILISFSIGQEQRGAITV
                     AVVIGLTSWVWTARAVRSQVISLRNRDHVN"
BASE COUNT          645 a          562 c          616 g          790 t
ORIGIN      
        1 acgacatctt catgatgtgg tctgatggtg ctggtcctac acagccttgg tctcgtatcc
       61 gccacctcat cagctctgag ttcgttggtg atgcggtcaa ctggaacggt aactggggtc
      121 agtactccaa cccggcagct gacgaattga tcaacgcaat tccgaatgag agcgatcctg
      181 ccaagttgaa cgaaatgtac accgaattgg tcaagatcta ccttaccgat gtaccttcct
      241 tcacactcat gtaccgcccg caggcgttcc acactgtgaa cgaaagcgta tggaccaact
      301 tcccgcatgg cgaagatggc accaacccgc ctgtaccccc actcgatcta acagacggct
      361 ggagcatcgc aggtctctat aacctgacgc tcgtcaaccc gtaatcaagt ttgtgttcaa
      421 aaacagccat gttccatcct gtatggggca tggctgttct tgtaaccatc atgatgataa
      481 aagcgtctac cttcaaaaat catcccttgc tctgcttaag aaattcttct aagaacattt
      541 cctctctttt ttagaagaga gtaggggagg ttttttgacg agagaccaag aaaactgaag
      601 atagcaattt aagtgaaatc tcttaaaata agcgatagta gataaaagca acccgctgaa
      661 tacatggagg tgtgtgttga aaggatattt gaagtatttt ctaaataaat tggggtggtt
      721 tgccattacg tttctctttg cgtttattct aaatttcctt cttccgcgcc ttatgcccgg
      781 tgaccctgtt gctgcgattg tttcgcgtat ggcgcaggga atgagtaaca cctctggtgt
      841 gcaggcgatt tatgaacagt acacagaatt gttcggcacg aataaaccga tgatcgaaca
      901 atttttgatt tatgtaaaga atgtcgctcg tggcgacttc ggtttttctt tcagccaata
      961 tcctagaaca gtatctgatg tcattggtgc ttccatttgg tggacacttg ctttgcagtt
     1021 ccctgccatt attgtgggat ggattatcgg aaatactttg ggcgctttag cagcttatct
     1081 taagggcggt tttgataaga tccttatgcc gattagcatt ttcgtcagta attttccggc
     1141 attcggtatg gcggttattt tcatggttat ttttggggtc aacctcaaat ggttccctac
     1201 ctcaggtgga tatggtttcg atatgattcc aaatttcagc tgggcattta ttaaatcggt
     1261 cattattcac tatcagttgc cattctggtc aattgtagtg attgccattg gtggacaggc
     1321 aattggtatg cggtctatgt ctatttatga attgaatgca gactatgtga agtatgcccg
     1381 ctttatgggt attcgtgata gcaagatcgt tggctatgtg ttccgaaatg ctatgcttcc
     1441 gcaggtgacc ggtttggcat tgtctgttgg aacaatggtg ggtggtgcac ttgttgctga
     1501 gattgtgttc agttaccccg ggcttggcac aaccctgttg cgagctgtgc taggacagga
     1561 ctatccattg atctccgcat caaccttgat catcaccgtt atggcattat ttgccaattt
     1621 cattattgag atcttgtatg gcttgatcga tccgcgcatc aaagctgcac aatctgacta
     1681 agtggagaaa ccagcatgaa acatataatc caacaggttt ttcgctcggg taagttcgtt
     1741 acaggcttct ctattttttc agccatcatt ctgattgtga tcttttatcc attgatcatt
     1801 accgatcagc ctttggcaat tattggtcaa ggtacgttct ttcctccagg aatttacgta
     1861 agtgtatacg acagtatgag cgcgaacgca tacacactca acttagatga agcagctgaa
     1921 atgcgtattg ctagtaagct aaatgatgaa gatcgtctgg ctgtaaaaga atggttggtt
     1981 gctgctggca ttccagaaag tgacattgat attacagata cggcaaaact tctggatcag
     2041 tggttcgcaa attacgactc gaaaaagaat gtgcctggca tgacgaatgc taaacgtaat
     2101 tactacattc gcctcaacaa cgcccttaaa gggttgcttt ctgctgaagc ggcgacggtg
     2161 atgcagacca gcccgcagac caatgaacta gagcagattg atactattca acagacagat
     2221 tatgtgaatg tcggcgaagt tgccaatgtg agagtgctac cgcttggtac agataacttt
     2281 ggcagagatg tgctaactga gctggtctct gcaacccgtg tctctttgca gattggtctt
     2341 gtggcaggaa taattgccac cattatcggt ctgaccttgg gcttgctcgc gggctatatt
     2401 ggcgggttgg tagatgacct catcatgttt gtcacgaacc tttttattgt catcccaccg
     2461 ttcgtgttat tgatcctcat ctcatttagt attgggcaag agcaacgtgg tgcaattaca
     2521 gttgcagtag tgattgggct tacctcatgg gtgtggacgg cacgtgctgt ccgttcgcag
     2581 gtcatttctt tgcgaaaccg agatcacgtc aat
//
LOCUS       DAOREF010000435         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28348_length_2596_cov_3.702479, whole genome shotgun sequence.
ACCESSION   DAOREF010000435 DAOREF010000000
VERSION     DAOREF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28348_length_2596_cov_3.702479"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2523
                     /gene="polA"
                     /locus_tag="PKI33_09530"
     CDS             <1..2523
                     /gene="polA"
                     /locus_tag="PKI33_09530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNM37294.1"
                     /translation="EMVDAFNIPRLEMDGYEADDVLGSVAKLAAEKGLGVKIITGDRD
                     LLQLVNERTAVYLAGDDQTYITDADVIKKLGVRPNQVVDYKAIVGDTSDNIPGVKGVG
                     EKTAVALLEKFNTLDAIYANLDQVENRWRGKFETNKETAYMSRDLAQIETNLKIKFDF
                     EHAKVAPFDGSKLEAFFKEMEFRTLVSKVPAISGGVASASAEIKAPTVKTGKTKAGQM
                     TMFAEEAQPVAVAIKPASIEVVIVDSAEKLKSLANELEKAKVISFDTETTGTEEMTAE
                     LVGISLAVKEGQGYYIPVGHVAGDNLPIQTVIDAITPAMTNAKIGKVAHNAKYDYIVL
                     ARYGLTVSPLTFDTMLAEFIVDPASRHLGLKNLSEQRLGEEMTHIEELIGKGKKQISM
                     AEVAIEAVANYAAADAETTLRLMAIEEKELKRVEGQKILDEIDMPLTAVLAEMEMTGA
                     LIDTKFFGEMSKELAARLNEIEKEIYGHVGKTFNINSPQQLSDVLFNHLRLEPPDKGR
                     KTSTGFYSTSADVLDSMRGQHPVLDFILEHRELSKLKSTYVDALPAAVDANTGRVHTS
                     YSQIGAVTGRLSSNNPNLQNIPIRTETGRKVRNGFIADKGNLLLSVDYSQIELRIVAH
                     MAQDEAMLAAFRAGEDIHATTAAAVYDIPLEQVNKDMRRHAKAINFGLIYGMSAFGLM
                     HSTGLTLAESEDFVKAYFKKFPGIKKYLDGIRRDAAEIGYVETLLGRRRYFPALQTKT
                     NVQLKNREEREAINAPIQGTAADIMKIAMLNIPPALQKAGLKAKMLLQVHDELVLEVP
                     KKEIEQTAKLVQETMANAYPMSIPLETEARAGVSWGEMTVLE"
BASE COUNT          680 a          612 c          770 g          534 t
ORIGIN      
        1 gagatggtgg atgccttcaa tattccgcgc ctcgaaatgg acggctacga agccgatgat
       61 gtgctgggca gtgtggcaaa gctcgcggcg gaaaaaggtc tcggcgtgaa aatcattacc
      121 ggagacagag acctgctaca gcttgtcaat gagcggacgg cggtgtatct ggcaggtgat
      181 gaccaaacgt acatcaccga tgccgatgtc atcaaaaagc tgggcgtccg cccgaatcaa
      241 gtagtggact ataaagccat cgtcggcgat acgtcggaca atattcccgg cgtaaagggc
      301 gtgggcgaaa agacagcggt tgccttgctg gaaaagttca acacgctgga tgcgatctat
      361 gccaacttag accaagtgga aaatcgctgg cgcggtaaat tcgagacgaa caaagaaacg
      421 gcgtatatga gccgtgacct ggcgcagatt gaaacgaacc tcaagatcaa attcgatttt
      481 gaacatgcca aggtggcacc cttcgacggc tcgaagttgg aagctttctt caaagaaatg
      541 gaattcagaa ctctggtaag caaagtgcct gccattagcg gcggtgtggc ttctgcctca
      601 gcagagatca aagcccccac tgtgaaaacc ggcaaaacca aagccgggca gatgaccatg
      661 ttcgcagaag aggcgcaacc cgtggcggtg gcgatcaaac cggcgagcat tgaagttgtg
      721 attgtggata gcgccgagaa actgaagagc ctcgccaacg agttggagaa agccaaggtc
      781 atttcgttcg atacggaaac gacaggcacc gaagaaatga cggctgaatt ggttggcatt
      841 tcactggctg tgaaagaagg gcaaggctat tacattccag ttggacatgt ggctggcgat
      901 aacctgccga ttcaaaccgt gatcgatgcg atcaccccgg cgatgacaaa tgcgaagatc
      961 gggaaggtgg cgcataacgc caagtacgat tacattgtgc tggctcgcta tgggctgacc
     1021 gtctcgccgt tgacctttga tacgatgttg gcagagttca ttgtggaccc tgcttctcgt
     1081 catttgggat tgaaaaatct ctctgagcag cggcttggcg aagagatgac ccatattgaa
     1141 gagctgatcg gcaagggcaa aaagcaaatt agcatggcag aggtggcgat tgaagcggtt
     1201 gccaattatg cggctgccga tgcagagacg accctgcgtt tgatggcaat tgaagagaaa
     1261 gaactcaaac gcgtggaagg gcaaaagatt ttggatgaga tcgacatgcc gctgacggct
     1321 gtgctggctg aaatggaaat gaccggcgca ttgatcgata ccaagttctt cggcgagatg
     1381 tctaaagaat tagcggcgcg cttgaacgag atcgaaaagg aaatttacgg tcacgttggc
     1441 aagaccttca acatcaactc gccacaacag ctttcggatg tgctcttcaa tcacttgcgc
     1501 ctcgaaccac ccgataaggg acgcaaaaca tccacgggct tttactccac ctcagccgat
     1561 gtgctcgact cgatgcgcgg acaacatcca gtgttggact ttatccttga gcatcgcgaa
     1621 ctctcgaagt tgaaatccac gtatgtggat gcgttgcccg ccgctgtgga tgcgaacacg
     1681 ggcagggtgc atacctcgta cagccagatc ggcgcggtga cggggcggct ctcttccaac
     1741 aacccgaact tgcagaacat ccccatccgc actgagacgg ggcgaaaagt gcgcaatggc
     1801 ttcattgccg ataaaggcaa tttgctgctc tcggtggact actcgcagat cgaactgcgc
     1861 attgtggcgc atatggcgca agatgaagcg atgctggcgg cgttccgcgc aggcgaagat
     1921 attcatgcca cgacggcagc ggcggtgtac gatattccgc tggaacaggt caacaaggat
     1981 atgcgccggc atgccaaggc gatcaacttc gggttgatct atggcatgtc tgcttttggg
     2041 ttgatgcatt cgacggggct gacgctggct gagtctgaag attttgtgaa agcgtatttc
     2101 aagaaattcc cgggcattaa gaaatatttg gatggcattc ggcgcgacgc ggcggagatc
     2161 gggtatgtgg agactctgct tgggcggagg cgctacttcc cagccttgca gaccaagacc
     2221 aacgtccagt taaagaaccg cgaagaacga gaagccatca acgcccccat tcagggcacg
     2281 gcggcagaca tcatgaagat cgcgatgttg aacatcccac cggctttgca gaaggcgggc
     2341 ttgaaagcca agatgctgtt gcaagtgcat gatgagttgg tgttggaagt gccgaagaaa
     2401 gagatcgagc agaccgccaa gcttgtgcaa gagacgatgg cgaacgccta cccaatgagc
     2461 attccgttgg agaccgaagc gcgtgccggt gtgagctggg gcgagatgac ggtgttggaa
     2521 taataggaag gggcaagccg gttttcttgc ccctttttgg cgggacttgt ataatgacgt
     2581 tatgcaaaat aaaggg
//
LOCUS       DAOREF010000436         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28498_length_2588_cov_3.021319, whole genome shotgun sequence.
ACCESSION   DAOREF010000436 DAOREF010000000
VERSION     DAOREF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28498_length_2588_cov_3.021319"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKI33_09535"
     CDS             complement(<1..285)
                     /locus_tag="PKI33_09535"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNM37295.1"
                     /translation="MPNRIATASIDIAAPIHVVWAAMLDFDAYPEWNPFIYKVSDLPE
                     TLTLGSRFLLHVRWANGSSYRSWETLTQLEPPAPSRDGVHTALLTYRYASW"
     gene            complement(318..1331)
                     /locus_tag="PKI33_09540"
     CDS             complement(318..1331)
                     /locus_tag="PKI33_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNM37296.1"
                     /translation="MPEYKSLDGNTPEGKRTAQNLIRLRFELDNAIPARTVDSTLLLA
                     TWNIREFGGSKSDGREAEPLYYIAEIISHFDMVAVQEVRDNLDALDELMKILGPWWQY
                     LVSDVTQGAQGNDERHAFIYDTRKIAFGGLSGELVPEMKKNKDGTLASDFAFARTPYV
                     AGFKAGWFKFTLCTQHFYYGDEKPDDPQRLKESQNVVKLLKKRMQSKDSWANNSILLG
                     DFNIFSVKDETFQAIEKESFQIPAKLKGTYSNANRDKPFDQIAFLAKDVEKQLGLAKA
                     GTFPFFDFVYRDDDLNTYQPGSTLAKYKQWRTFKMSDHLPLWVELYVDFGEAYLQKKM
                     GGG"
     gene            complement(1455..1970)
                     /locus_tag="PKI33_09545"
     CDS             complement(1455..1970)
                     /locus_tag="PKI33_09545"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNM37297.1"
                     /translation="MDNAEIKAILQNLGERVPPSSQLILLGGSALALLGSPRLTVDID
                     FIGDDVQPNPLHRIIMQIARDLQIHIEPVPLERFIPLPEGNEKRRIRIGQYGNLEVFI
                     ADPYSIALSKVDRGFETDIEDILFLIQKNFITLAELEHITRKAISQAQGFDLSPEILE
                     HLQDLKNQLES"
     gene            complement(1972..2466)
                     /locus_tag="PKI33_09550"
     CDS             complement(1972..2466)
                     /locus_tag="PKI33_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37298.1"
                     /translation="MSQTAVITGDQISDALRALDVHFILGGNRQSAFIHKSPTRLIAA
                     LAMSNEARLRLSLIPLFLEHPEFAIHVKSVAANLLPSAQLTLQCYYTAALWLAQKYQL
                     QSSLPDHFSLELGLVPSTDVDENLRELAKRHKELSGRRVNWLGTYQHAAQIWQKGLEY
                     KKGS"
BASE COUNT          591 a          631 c          710 g          656 t
ORIGIN      
        1 ccaagaggcg taacgataag tcagcagcgc ggtgtggacg ccatccctgc ttggggcagg
       61 gggctcgagt tgggtgagag tctcccatga gcggtagcta gagccgtttg cccagcgcac
      121 atgcaaaagg aagcgtgagc cgagcgtcag tgtttcaggc aggtcgctga ccttgtagat
      181 aaagggattc cattcagggt aggcatcaaa atccaacatc gccgcccaca ccacatggat
      241 gggggcagcg atgtctatag aagcagtcgc aattcgattc ggcatgcccc cattatacag
      301 actcagcctt gcactcttta cccgccgccc attttctttt gcaaatacgc ctcaccgaag
      361 tcgacatata gctcgaccca taagggcaga tggtcagaca ttttaaaggt gcgccattgc
      421 ttgtacttcg ccagcgtgct gccgggttga taggtattca ggtcgtcgtc gcggtagaca
      481 aaatcgaaga aggggaaggt gcccgccttg gcgagaccga gttgtttctc cacgtctttg
      541 gcaaggaagg cgatctgatc gaagggctta tcgcggttgg cgttgctgta ggtgcctttg
      601 agcttggcgg ggatctgaaa agactctttc tcgatggctt ggaaggtttc atctttgacc
      661 gagaagatgt tgaaatcgcc caacagaatg ctgttgtttg cccaactgtc ttttgattgc
      721 atacgcttct tgagcagctt caccacgttc tgcgactctt tcaggcgctg tgggtcatcg
      781 ggcttttcgt cgccgtagta gaagtgttgc gtgcagagcg tgaacttgaa ccagccggct
      841 ttgaagccag ccacataggg tgtgcgcgca aaggcaaagt ctgaagcgag cgtgccgtct
      901 ttgttcttct tcatctcggg caccagttcg cccgaaagcc caccgaaggc gatcttgcgc
      961 gtatcgtaga tgaaggcatg gcgttcgtcg ttgccctgcg ccccctgggt gacgtcggac
     1021 accagatact gccaccacgg accgaggatc ttcatcagct cgtccagcgc atccaaatta
     1081 tcgcggacct cctgcacggc gaccatgtcg aaatgcgaaa tgatctcggc gatgtaatac
     1141 agcggctcgg cttctctgcc atcggacttg ctaccgccaa actcgcggat gttccaggtg
     1201 gcaagcagca gggtcgagtc gaccgtgcgg gcagggatgg cgttatccaa ctcgaagcgc
     1261 aagcggatca ggttctgggc ggtgcgtttc ccttcggggg tgtttccatc gaggctttta
     1321 tattcaggca tgtcggcttt cctctctttc aacaaagaat atacggctta tttcagtata
     1381 gccatttcac agccccattt caaatggtat ttttcaagtg acagtcacct cgcaggtgac
     1441 tgtcacttat gtactcatga ttcgagttgg tttttcaaat cctgaagatg ttcgagaatt
     1501 tcagggctta gatcaaaacc ttgcgcctgc gaaatggctt tgcgggtgat atgttccaac
     1561 tctgcaagtg ttatgaagtt tttctggatc aaaaagagaa tgtcttcaat atcggtttca
     1621 aagccacgat cgactttact gagggctatg ctgtaagggt cggcaataaa aacttcgagg
     1681 ttcccatatt gaccgatacg gatacggcgt ttttcattcc cttcgggcag gggaatgaag
     1741 cgctccagtg gaacgggctc gatatggatt tgcagatcac gagctatttg catgatgatg
     1801 cggtgaagtg gattgggctg aacatcatct ccgataaagt cgatgtctac tgtcaggcgt
     1861 ggacttccga gcaaggcaag ggcgctacca ccaagaagaa ttaattgaga ggaaggcgga
     1921 acccgttccc ccaagttttg taaaatggct ttgatttccg cattgtccat gttatgagcc
     1981 ttttttatac tccagccctt tttgccagat ttgcgcagca tgttggtagg ttcccagcca
     2041 gttgacccgc ctgccgctta gttctttgtg acgtttcgct agttcccgca ggttttcatc
     2101 tacatcggtg ctgggaacca gaccaagctc aagagaaaaa tggtcaggca ggctactctg
     2161 cagttgatat ttttgtgcca gccaaagcgc ggcagtgtaa tagcattgca aggtaagctg
     2221 tgccgaaggg agcaggtttg cagcgacgct ttttacatgg atggcaaatt ctggatgctc
     2281 aagaaagaga gggatgagtg ataggcgcaa acgggcttcg ttgctcatgg cgagggcggc
     2341 gatgagacga gtgggtgact tgtggatgaa ggcactttga cggttcccac ctagaataaa
     2401 atgcacatcc aacgcgcgta aggcatcgct gatttgatcg cctgtaataa cagcagtttg
     2461 gctcataggg aaattatagt atgaaatatg gaagtgcctc ttacaactcc ataccttgca
     2521 acacctctcg caacgcggac agttcctcag cggacaaggt cacgcctttg cccatcttgc
     2581 tatggtcc
//
LOCUS       DAOREF010000437         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28538_length_2586_cov_2.753062, whole genome shotgun sequence.
ACCESSION   DAOREF010000437 DAOREF010000000
VERSION     DAOREF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28538_length_2586_cov_2.753062"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /locus_tag="PKI33_09555"
     CDS             <1..2153
                     /locus_tag="PKI33_09555"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNM37299.1"
                     /translation="LPQALAGDTNLVIIGQPGTGKTVALAHLASLAANRSEKLGALQS
                     FVPFHFHVADLNLPIQDPKNILNVISDAAAENAPMFDVGKIPTFVQSVFRSGEVLLLV
                     DGYDEITPDEQTLVAEFFKLLLKAHPKTRIVTTGAPEYLDGLIPLGFVPLSLCTWTEK
                     GNQEFVDQWGELWSRTVAMEAWAQTGPEQVDPLLINSWLGSASLNLSPLELTLKIWGA
                     YAGDSLGPNILDTIATHIRRIAPANTPLAALETLAMQVVVSATPVFDPRKAREWVKSF
                     EVAEETPEGETAQSPEGEAQPSEDAKSKKGKKVEKVATPNTGLLGKMTESGLLVSYPN
                     NRMRFIHSVLAGYLAGHALTNYNAEETLLNQPDWSGKYLTMRFIAAHGDASRLVQAML
                     EFSRLPMHRPLFAAARWLRDAPKNAPWRGKMFGTLAAILQTDGIPLSLRGQAMAAFVY
                     SNDVSAATLFRQFATSSSFELIHLAILGLGAVRDTKSLKILEGALNAPSLAVKRAACM
                     ALAAINTNESLEIAAHALLNGEEDVRRAAGEALANDPKEGHAMLRDGITINDILLRRA
                     VIYGLGRVREEWATDLLKKVQIEDEQWVVRNAATEVLDMQAQSYAYAPMKPKAPHETP
                     WLIEFAAKKGVGVSPGVPATDILLVALKDENPETRLAAIQFLKYTPQEIVLKQLYEAM
                     YKDDPELREATFNILWELGAAGVKLPHPSEYGLN"
     gene            2155..>2586
                     /locus_tag="PKI33_09560"
     CDS             2155..>2586
                     /locus_tag="PKI33_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNM37300.1"
                     /translation="MLITNANIITWESPNRVLSNYAILIEGGKIKEIGTTKSLTAKHP
                     KVKATDVNGQYVMPGGICAHTHFYGAFARGMAIPGPAPKDFPEILKKLWWPLDRSLDA
                     EGVRYSALVSIVDAIKHGTTTLIDHHASPNTIDGSLDVIADV"
BASE COUNT          670 a          660 c          621 g          635 t
ORIGIN      
        1 ctctgccaca agccttggct ggggatacaa acctcgtcat tatcggtcag cccggcactg
       61 gcaaaactgt agctctcgct catttggcaa gccttgccgc aaaccgcagt gaaaaattag
      121 gtgcactgca aagcttcgtt ccgtttcact ttcatgtggc agacttaaat ctgccaattc
      181 aagaccccaa aaatatactg aatgtcatta gcgacgctgc cgccgaaaat gcacctatgt
      241 tcgatgtcgg caaaattccg acatttgttc aatccgtctt tcgaagcgga gaagtgcttt
      301 tactcgttga tggctacgac gagattacac ccgatgaaca aaccttggtg gcagagttct
      361 tcaaactatt gctcaaagct catccaaaga cgcgcatcgt cacaactggt gcgccagaat
      421 atttggatgg tttaattccg cttggctttg tgccgctaag tctgtgtaca tggacagaaa
      481 aaggcaatca ggaatttgtg gatcaatggg gcgagttatg gtctcgcacc gttgcaatgg
      541 aagcttgggc acaaacggga cctgagcaag tggatccgct tcttatcaac tcctggcttg
      601 gttctgcgtc tctaaattta tctccccttg agttgactct caaaatttgg ggcgcatatg
      661 caggcgatag tttaggtcct aacattctcg ataccatcgc cacccatatt cgacgcatcg
      721 ccccagccaa cacaccgctt gccgcgcttg agacgcttgc catgcaagtt gtcgtcagtg
      781 ccacgcccgt atttgacccg cgtaaggcgc gtgaatgggt gaagtcgttt gaagtggcag
      841 aagaaactcc tgaaggcgaa acagctcaaa gtccagaagg tgaggcgcag ccatcagaag
      901 atgccaagtc taaaaaaggc aagaaggtcg aaaaagttgc tacgcccaat acaggtctgc
      961 tcgggaagat gaccgaaagc ggattgttag tgtcatatcc caataaccgt atgcggttta
     1021 ttcattcggt attggctgga tatcttgcgg gtcatgcatt gaccaattac aacgccgaag
     1081 aaacattgtt gaatcaaccc gattggtctg gaaaatatct caccatgcgg tttattgccg
     1141 cacatggtga tgccagccga ttggtgcaag ccatgctcga attttcacgc ctacccatgc
     1201 accgaccgct atttgcggcg gctcgctggc tacgtgatgc gcctaaaaat gctccatggc
     1261 gcggaaaaat gtttggaacc cttgccgcga tcttgcaaac agatggcatt cccctcagcc
     1321 tgcgcggaca agccatggca gcctttgtgt atagcaatga cgtcagcgct gcaactttat
     1381 tccgccagtt tgccacatca agttcattcg aactgattca tcttgccatt cttgggcttg
     1441 gcgccgtacg cgatacgaaa tccttgaaga ttcttgaagg cgcattaaat gctccgagcc
     1501 ttgctgtaaa acgggcagcg tgtatggcat tggcagccat caacaccaat gagtcgcttg
     1561 aaattgccgc acatgctttg ttgaatggcg aagaagatgt gcgtcgtgcc gcaggtgaag
     1621 cccttgccaa tgatcccaaa gaaggtcacg ctatgttgcg cgatggtatt accatcaacg
     1681 atattctcct gcgccgtgca gtgatctatg gtcttgggcg tgtccgcgag gaatgggcga
     1741 ctgacctgct taagaaagtt caaattgaag acgaacaatg ggttgttcgt aatgccgcta
     1801 cagaagtgtt ggatatgcag gcacaaagct atgcctatgc acccatgaag cctaaagctc
     1861 cgcatgaaac cccatggctt attgagtttg ccgccaagaa aggcgtgggc gtttcacccg
     1921 gtgtccctgc cacagatatt ttgttggttg ccctgaaaga tgaaaaccct gaaactcgct
     1981 tggctgccat tcaattctta aagtacacac cacaagagat tgtgctcaaa caattgtacg
     2041 aagccatgta taaagatgac cccgaattac gggaagcaac cttcaacatc ctttgggaac
     2101 ttggcgcagc gggcgtcaaa ttaccgcacc ctagcgaata tggattgaac taatatgctg
     2161 atcacaaacg caaacatcat cacatgggaa tctcccaatc gcgtcctctc aaattacgcc
     2221 attcttattg aaggcggaaa gatcaaggaa atcggcacaa ccaaatcgct tacagccaaa
     2281 catcccaaag taaaagccac tgacgtaaac ggtcagtatg tgatgccggg cggcatttgc
     2341 gcccacactc acttttatgg tgcttttgcg cgcggcatgg caattccggg tccggcgcca
     2401 aaggattttc ctgaaatcct caaaaaatta tggtggccac ttgaccgttc tcttgatgca
     2461 gagggcgtgc gatactctgc cttggtttct atcgtggatg caatcaagca tggcaccact
     2521 actctcattg atcaccatgc atctcccaac actattgacg gctcgctaga cgtcattgct
     2581 gatgtg
//
LOCUS       DAOREF010000438         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28573_length_2584_cov_3.933175, whole genome shotgun sequence.
ACCESSION   DAOREF010000438 DAOREF010000000
VERSION     DAOREF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28573_length_2584_cov_3.933175"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /locus_tag="PKI33_09565"
     CDS             <1..2111
                     /locus_tag="PKI33_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNM37301.1"
                     /translation="VNLVQKPKHTILGILSYTSIVEACDDVPRLLAMKPSAVELIPQL
                     ILRNARSIPAYARQMGWVIGDPAAVLVVEFSGDQPSALKEAVRKLGDVLTIAESPEDQ
                     ARIWNIRKVGLGILDSRPTSARPIAFIEDCAIPVERLGEFVREVEQILNAHGTFGGIY
                     AHASAGCLHIRPVLDLQLGEAVRNMRNIIEQVFALTMSLGGAMSSEHGDGIARGEFIE
                     RTYGREVTEAMRLLKKAADPDNLLNPKKLFDAPPMDTNLRYGETYQAQAWKPVLTFEH
                     ERGLEGAVESCNGQGVCRKTNGVMCPSFQATRDEAFSTRGRANLLRALMRSDVDFSVP
                     RNSELELATYQSLDLCLACKGCTSECPSGVDMPKLKYEFMNQYYKSHPRHLRDYLFGY
                     FHVATKLLMPFAPMANWFMQIGWSKKIVARVLGISEHRPFPKFVRGARQAHEMQFDKQ
                     VIFVSDVFSHYIEPEVEEAVVEILNSLGYEVKILPTIGAGASLLSKGFLNAAQRHAEK
                     VLAEIKRLDGGAGLSVVGCEPPELYCLKHEYESLLPNRKEEIESIKKRTWLVDEFIMR
                     VVNEAKMDISHVDSSEQNNRLTLHPHCHHRAEGPAADGLPSGVSATVDMLKQFGYEVD
                     VIDAGCCGMAGTFGYDAEHYELSMKVGELGVLPKVRDKKLENRELVASGAACRMQIKQ
                     GADVDARHPLLLVKERLISK"
     gene            2118..>2584
                     /locus_tag="PKI33_09570"
     CDS             2118..>2584
                     /locus_tag="PKI33_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37302.1"
                     /translation="MLNRLSNFFYAVSTGWLTLTGLVVFILFMIFVLPQQAQKAEAYS
                     GGTSPDTSYIYSASDLYQMAESYGAEGRAAYIYARFTFDLVFPLAYLFFLTTSISWLL
                     ARGLSENSRWRMLNLFPLAGAIFDYLENISTSLVLARYPSQTPVVDVLAPVFTA"
BASE COUNT          643 a          511 c          689 g          741 t
ORIGIN      
        1 aagtcaacct tgttcaaaag cctaagcaca caattcttgg aatcttgtcg tataccagta
       61 tcgtagaagc gtgtgacgat gttcctcgct tgttggcaat gaagcctagc gcagtggagt
      121 tgattccgca gttgattctg cggaatgcac gtagtattcc ggcgtatgcg cggcagatgg
      181 gttgggtgat tggagacccg gcagccgtgt tggtagtgga atttagtggc gaccaaccgt
      241 cggctttgaa agaagcggtg cgtaagcttg gcgatgtgtt gactattgca gagtcgcctg
      301 aagatcaggc tcgcatttgg aacatccgca aagtgggctt agggatttta gactcacgtc
      361 caacgtctgc cagaccaatt gcatttattg aagattgcgc tatccccgtt gaaaggttgg
      421 gggagtttgt tcgtgaagtt gaacagattc ttaatgctca tgggactttt ggcggaattt
      481 atgcacatgc ttcggcaggc tgtttgcata ttcgccctgt gttagatttg caactgggtg
      541 aagctgtgcg gaacatgcga aacattattg agcaagtttt tgcgttgacc atgtctttgg
      601 gcggtgccat gagcagcgaa catggcgatg gaattgcgcg tggggaattc attgaacgaa
      661 cttacgggcg tgaagtgacc gaagccatgc gcttgctgaa gaaagccgca gaccccgata
      721 atcttttgaa ccctaaaaaa ttgttcgatg cgccacccat ggatacgaac ttacggtatg
      781 gcgaaacgta tcaggcgcag gcttggaagc ctgtattgac ctttgaacac gagcgaggtt
      841 tagaaggggc agttgagtca tgcaatgggc aaggcgtatg tcgtaaaacg aacggtgtga
      901 tgtgcccatc gtttcaggca acgcgcgatg aagcctttag tacgagaggg cgagctaatt
      961 tactgcgtgc attgatgcgc tcggacgttg atttttcagt gcctcgaaat tctgaacttg
     1021 aattagcaac ctatcagtca ctcgatcttt gccttgcttg taaaggctgc acatccgaat
     1081 gcccaagtgg tgtggatatg ccgaaactca aatatgagtt tatgaatcaa tattacaaat
     1141 cgcatccacg ccacttgcga gattatctgt tcggctattt tcatgtcgct acaaaattgc
     1201 tgatgccatt tgcccctatg gcaaattggt ttatgcaaat aggctggtcg aagaagatcg
     1261 tggcgcgtgt gttgggtatt tcagaacaca gaccttttcc aaaatttgtt cggggtgcac
     1321 gccaagcgca tgaaatgcaa tttgataaac aagtaatttt tgtttcggat gttttctcgc
     1381 attatattga acctgaggtt gaagaggctg ttgttgagat tttgaactcg ttggggtatg
     1441 aagtgaaaat tttgccaact attggagcag gcgcttcttt gctttcgaag ggatttctca
     1501 atgctgcaca gcgccatgca gaaaaagtgt tggcagaaat taaacgcttg gatggtggag
     1561 caggtttgag cgtagttgga tgcgagccgc ccgagttgta ttgtctcaaa catgaatatg
     1621 aatcgttatt gccaaatcgc aaagaggaaa ttgaatccat aaagaagaga acatggttag
     1681 tggatgaatt tatcatgcgc gtagtcaatg aggctaaaat ggatatttca catgtggact
     1741 cttctgaaca aaataaccgc ctcactctcc acccgcactg tcaccaccgc gcagaaggtc
     1801 ccgccgctga tggtttacct tctggcgttt cagctacagt agatatgctc aagcaatttg
     1861 gctatgaagt ggacgtaatt gatgcaggtt gttgtggcat ggcaggcacc tttggctatg
     1921 atgctgaaca ttacgagcta tcaatgaaag tgggggagtt gggagtgtta ccgaaagtga
     1981 gagataagaa attagaaaac agagaattgg tggcgagtgg tgcggcatgt cgtatgcaga
     2041 tcaagcaagg tgcagatgtg gatgcacgtc atccattgtt attggtaaaa gaacgactta
     2101 tatccaagtg aggtgctatg ttgaaccgac tttcaaattt cttttatgct gtttctacag
     2161 gctggctgac cctgacgggg ttggttgtct ttatcctttt tatgattttc gtattgcctc
     2221 aacaggctca aaaagcggag gcgtatagcg gtggaacttc accagacact tcatatattt
     2281 actctgcaag tgacctgtat cagatggcag agagttatgg cgcagaaggg cgggctgctt
     2341 atatttatgc acgtttcaca ttcgatttgg tttttccgtt ggcttactta ttttttctga
     2401 caacatctat aagctggttg ctggcgcgtg gactttctga aaactcccgc tggcggatgt
     2461 taaatctctt tccgcttgcg ggagctattt ttgattactt ggaaaatatt tccacttcac
     2521 tagtgttagc acgttacccg tctcagacgc ctgtggttga tgttcttgca ccggtgttta
     2581 cggc
//
LOCUS       DAOREF010000439         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28705_length_2578_cov_4.742370, whole genome shotgun sequence.
ACCESSION   DAOREF010000439 DAOREF010000000
VERSION     DAOREF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28705_length_2578_cov_4.742370"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKI33_09575"
     CDS             complement(<1..596)
                     /locus_tag="PKI33_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNM37303.1"
                     /translation="MVKVTNLDKYFGRLHVLKDISLEVPERQVLCLIGRSGSGKSTLL
                     RCLNFLEDPSRGIIEVDGIKVDAEVKNKERRQLIHDVRLKTGMVFQEFNLFPHMTVLE
                     NVIEGPVTVKGMDKKVATELAEQNLDSVGMLFKKDEYPLRLSGGQKQRVAIARALTMQ
                     PRVMLFDEPTSALDPELIGEVLNVMKKVAYEGMTMIVVT"
     gene            complement(613..1461)
                     /locus_tag="PKI33_09580"
     CDS             complement(613..1461)
                     /locus_tag="PKI33_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNM37304.1"
                     /translation="MASTTNQEQAPLSQAELLYQAQLRKERLFRTHVGVSWAVLFFIL
                     IFLFSGQTIQLGSFKFDTIELDSSFILKNLSFIAQGLGQTLLISVLSILFAMILALLA
                     ALGRLSTFPPIYAISTFYVSLIRGTPLYLQIFFFFLALPQLGIILSGIVAGVMALGLN
                     YGAYMSETFRAGLSSVGKGQREAAMALGMTPGQTMRRVILPQALRFAIPPMGNDFISM
                     TKDSALVSATGFVHELMWRATKVGRAQFNNLEALIMAAMFYWILTLILTYFQGKLEAR
                     LAKGDR"
     gene            complement(1539..2450)
                     /locus_tag="PKI33_09585"
     CDS             complement(1539..2450)
                     /locus_tag="PKI33_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNM37305.1"
                     /translation="MKKLLTLASLLAVASLVLAACGGGGGSSASDLLGAIQERGYILI
                     STDPNYEPQSFLNTEGTRPADTKCPSDALTTAEMQGFDVDVATAIGDSLGVETCFATP
                     SWDAITAGNWADKWDMSVGSMTVTTARQQILDFSVPYYYTPAVVAVAADAGIDSLEGL
                     SGQSVCAGAATTYEAWLNNDTEALGLPASSIYAQVPADVTVVPLETDQECAQAIASGR
                     KDFVAYVTSETVVDANLAAGMPVVKLDGAVYSEDLAAAFDKSSSLDTASLRAKVDELF
                     KAMHEDGRLSELSNKWFEVDLTAAPNQ"
BASE COUNT          682 a          598 c          697 g          601 t
ORIGIN      
        1 gtgacaacga tcatggtcat tccttcgtaa gccactttct tcatcacatt caatacttcg
       61 ccaatgagtt cagggtctaa tgcagaggtt ggttcatcga agagcatcac acgcggttgc
      121 atggtgaggg cgcgcgcaat ggcaacgcgc tgtttttgcc cgcccgatag gcgcaacgga
      181 tactcgtctt tcttgaaaag catgcctaca ctatccaggt tttgttctgc gagttcagtg
      241 gcgacttttt tatccatgcc ctttacagtt acaggacctt cgatcacgtt ctctaatacg
      301 gtcatgtgtg ggaagaggtt gaattcttga aacaccatgc cggtcttcaa acgcacatcg
      361 tgaataagtt ggcgacgttc tttgttcttg acttcagcat ctaccttgat gccatctact
      421 tcaatgattc cacgagaggg atcttcgaga aagttaaggc aacgcagaag cgtgcttttc
      481 cctgaaccgc tacgcccgat aagacacaga acctgacgtt caggcacttc aaggctaata
      541 tctttcaaaa catgcaaacg cccgaaatat ttatcgaggt ttgtaacttt tacaattgga
      601 agggtggaca tgttatcggt ctccctttgc aaggcgggct tccaatttgc cttggaagta
      661 ggtgaggatg agggtaagga tccaatagaa catggctgcc ataatgagcg cttcaaggtt
      721 gttgaattga gcacgcccta ctttggtggc gcgccacatc aattcgtgga cgaagccggt
      781 tgccgaaacg agagccgagt ctttcgtcat agagataaag tcattgccca tgggcggaat
      841 ggcaaaacgc aaagcctgcg gtaggatcac gcgcctcatg gtctgaccgg gagtcatgcc
      901 cagcgccatg gcagcttctc tttgcccttt gcctaccgag cttaagccgg cacggaatgt
      961 ttcagacatg taggcaccat aattcagacc caatgccatc acccctgcga cgataccgga
     1021 aagaataatg ccaagttgtg gcaaggcaag gaagaagaaa aatatttgca gataaagcgg
     1081 cgttccacgg atcagcgata cgtagaacgt actaatagca taaataggag ggaaggtgga
     1141 aagcctgcca agggctgcca aaagagctaa aatcattgca aacaaaatag agaggacgga
     1201 gatcaaaaga gtttgcccca acccttgtgc aatgaaagat agatttttta gaataaaact
     1261 gctgtctagt tcgatagtgt cgaacttaaa agaaccaagt tgaattgttt gcccactgaa
     1321 aagaaagatc aagataaaga acagaacagc ccaggaaacg ccgacatgcg tgcgaaacaa
     1381 acgttccttg cgaagttgtg cctgataaag caactcggct tgtgaaagcg gagcttgttc
     1441 ctggttggtg gtggaagcca tcttttacct ccgagaggtg aagtggaata ataaaaagag
     1501 aagcggagat gatccgcttc cctttttatt tcgttttctt actggttggg tgcagcggtc
     1561 aagtcaactt cgaaccactt gttggagagt tcggagaggc gaccatcttc gtgcatggct
     1621 ttgaagagtt cgtccacttt ggcgcgcaag gaagcggtgt caagggaaga ggacttgtcg
     1681 aaagcggcag cgaggtcttc agagtagaca gcgccgtcta acttgacgac aggcatgcct
     1741 gcggcgagat tcgcatccac aacggtttca gaggtcacgt aggcaacgaa gtccttacga
     1801 ccagaggcaa tcgcctgggc gcattcctga tcggtttcga gaggaaccac ggtgacatca
     1861 gcaggaacct gagcgtagat ggagctggcg ggcaagccaa gcgcttctgt atcgttgttg
     1921 agccaggctt cgtaggtggt ggcggcgcca gcacaaacgg actgaccgga aagaccttcg
     1981 agagagtcga tgccagcatc agcagcaaca gccacaaccg cgggggtgta gtagtagggc
     2041 acgctgaaat cgaggatctg ttggcgagcg gtggtgacgg tcatggagcc cacgctcata
     2101 tcccatttgt ctgcccagtt accggcagtg atcgcatccc aggagggagt agcgaaacag
     2161 gtttccacac cgagtgagtc accaattgcg gtggcaacat ctacatcgaa gccctgcatt
     2221 tcggctgtgg tgagggcgtc gctggggcac ttggtgtcag cagggcgggt cccttcggtg
     2281 ttcaagaagg actgaggctc atagttcggg tcagtagaaa tgaggatgta tccgcgttcc
     2341 tgaatggctc caagtaggtc tgaagcggaa gaaccgcctc cgcctccgca tgcagcaaga
     2401 acgagcgatg ctacagcgag aaggcttgca agggtcaaaa gtttcttcat ttttctcctc
     2461 cgagagaaat aaaaattatt ggcagcgatt tcgctgccac ggaatgctct ccactccgcg
     2521 aggttaagca tagactattt ttttcaattg tgcaaggatt ttagttatta aaaaacat
//
LOCUS       DAOREF010000440         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28748_length_2576_cov_4.715192, whole genome shotgun sequence.
ACCESSION   DAOREF010000440 DAOREF010000000
VERSION     DAOREF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28748_length_2576_cov_4.715192"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="PKI33_09590"
     CDS             <1..1461
                     /locus_tag="PKI33_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37306.1"
                     /translation="HVARNFPVIRFDAAMTLAKKHIQRLWFPEPGAGGAIPSRSQFGM
                     PKAEFDEKIPIEFWREVVDRVAAEVPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMH
                     MLRDEDNAKYRMAIKNTLEFDPQILKRYVNFMNNPDEKTAVEQFGKGDKYFGVCTVLA
                     TLPGLPMFGHGQVEGYSEKYGMEFRRPKWEETPDDGLIQAHDWRIFPLLHRRALFADV
                     ENFLLFDLQKPYSGLDENVFAYSNRLDDSQSGAIERGLVIYHNKFGDTKGWIKTSTAA
                     LDKASGKMKRRSLAEGLALPRRGYVIFKDYASQLEYVRSCEELWEKGMYVELGAYQCH
                     AFMDFRFVHDTEWAIICNNLKGAGVPSLQEEWRKTFAKVEEVKEEKPAKKKRTARKPA
                     VKKTAAKSTKKAVTKKPAAKKPKATVKKSATAKKPAKKMVTKKATASKPKAKKPTPKT
                     TKTVKAKKTVTKKAPVKKTTAKPVAKKKTVSKKKSK"
     gene            1517..1918
                     /locus_tag="PKI33_09595"
     CDS             1517..1918
                     /locus_tag="PKI33_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HNM37307.1"
                     /translation="MKLKLYLITFLAFLGIDSLWLGLVAPSFYKSQIGYIMAENPNFF
                     AAGLFYLLFIFGMVVFIVEPGVREQTLMQAVARGALFGLVTYATYDLTNLATLEGWPI
                     LVTVVDLIWGTVLSAAVTLVSVWAGKRFIKA"
     gene            2190..2432
                     /locus_tag="PKI33_09600"
     CDS             2190..2432
                     /locus_tag="PKI33_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37308.1"
                     /translation="MDYGMIGKLEKAKRYAEDRHRFRFNKFDLTFHGDNNEHHVGYDN
                     GVFSCDCEFFLTHKRCGHSMALEILLKDMIVETVEA"
BASE COUNT          730 a          562 c          633 g          651 t
ORIGIN      
        1 catgtcgcgc gcaacttccc ggtcatccgt tttgatgcgg cgatgacact tgccaagaag
       61 cacattcaac gcttgtggtt ccccgaaccc ggcgctggcg gagccattcc atcgcgttca
      121 cagtttggta tgcccaaagc agaatttgac gaaaaaattc caatcgaatt ctggcgtgaa
      181 gttgtggacc gcgtggctgc tgaagttcct gatacgcttt tgctcgccga agccttctgg
      241 ttgatggaag gatatttcgt ccgcacactt ggtatgcatc gtgtgtataa ctcggcgttc
      301 atgcacatgt tgcgtgatga agataatgcc aagtaccgca tggcgatcaa aaatacactg
      361 gagtttgacc cgcaaattct taagcgttat gtcaacttca tgaacaatcc cgatgaaaag
      421 accgcggttg agcaatttgg aaaaggggat aaatacttcg gcgtttgtac agtgctggca
      481 accttgccgg gtctgcccat gttcgggcac ggacaggttg aaggctattc tgaaaaatac
      541 ggcatggaat tccgccgccc gaaatgggaa gaaaccccag atgacggttt gattcaagct
      601 catgattggc ggatcttccc gctccttcac cgccgagcgc tgtttgccga cgtggagaat
      661 ttcttactct ttgaccttca gaaaccctac agcggactcg atgagaacgt ctttgcatat
      721 tccaaccgtt tggatgattc tcaatccggc gctatcgaac gtggattggt tatctatcat
      781 aataagtttg gtgataccaa aggttggatc aaaacctcta ccgccgcatt agacaaagct
      841 tctggcaaaa tgaagcgccg ctcgcttgcc gaagggcttg ccttgccacg ccgcggatat
      901 gtcatcttca aagattacgc ttcacagttg gaatatgtcc gctcgtgtga ggaactttgg
      961 gaaaaaggca tgtatgtgga attgggcgcg taccaatgcc atgccttcat ggatttccgc
     1021 tttgttcatg acacagaatg ggcgatcatt tgcaataact tgaagggtgc cggtgtgcct
     1081 tctcttcaag aagaatggcg caaaaccttt gccaaggttg aagaagtcaa agaagagaaa
     1141 cccgctaaga aaaaacgcac agcacgtaaa cctgccgtga agaagacggc tgccaagtcc
     1201 actaagaagg cagtgacaaa gaaaccagca gccaagaagc caaaagctac cgtaaagaag
     1261 tccgcgacgg caaagaaacc tgccaagaag atggttacaa agaaagccac ggcgtcgaag
     1321 ccaaaggcaa agaagccgac gcccaaaaca acaaagacag taaaggcgaa gaaaacggta
     1381 acgaaaaagg cgcccgtgaa aaaaacaact gccaaacctg tggcaaagaa aaagacggtt
     1441 agtaagaaga aaagcaagta gctcatctac tcggttagag aggaattctc taaccgagta
     1501 ttttttagga gacaccatga aactaaaact ctatctcatc acattccttg cctttctcgg
     1561 catagactcg ctttggcttg ggttggttgc gccgtcgttt tataagtctc agatcgggta
     1621 catcatggca gagaatccaa acttttttgc ggcgggattg ttttatttgc tgttcatttt
     1681 tggcatggtg gtctttatcg tggagccggg cgtccgtgag cagacgttga tgcaggctgt
     1741 agcacgcggt gcattgtttg ggttggtgac ctatgccaca tacgatctga ccaaccttgc
     1801 gacattagaa ggctggccca ttttagtaac agtagtggac ttgatctggg gaacggtctt
     1861 aagcgcggcg gtaacgctgg tcagtgtttg ggcggggaag aggtttatta aggcgtgaca
     1921 agcccaagtc tggtgtgatg cgcgtagtca gattagggtg tgatgaatat ttgtatatgt
     1981 ccaccctaca aacgccatac gaaatcatta cattacgaaa atcatattga cttgagtttt
     2041 gaaaagtact atactgacaa acaataagag gtaacgctga catgcgatta tttcacggaa
     2101 atacccggtt ttgtagataa agacacgcgt atatacacgc gtgtcttttt tatatctaat
     2161 aacaatcaaa caaaaaggag acttttgcca tggactacgg aatgatcggc aagctcgaga
     2221 aagcaaaacg ctacgcggag gatcgccatc gttttcgctt caataaattt gatttgacct
     2281 ttcacgggga taataatgaa caccatgttg ggtacgataa tggcgtcttt tcttgcgatt
     2341 gcgaattttt cttaacccac aaacgctgtg ggcattcaat ggcgcttgag atccttttga
     2401 aagacatgat cgttgaaacg gtcgaagctt aaccttacaa ttgaatagtt ttgctgccct
     2461 ccacatgcag tggggggcag tttttgttta agaaacattc tcattaaatc ttaaacaatg
     2521 tagagtaaaa ttttccttat gaagcgaatt aaccgtagag attttctaaa actagg
//
LOCUS       DAOREF010000441         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28888_length_2569_cov_4.171042, whole genome shotgun sequence.
ACCESSION   DAOREF010000441 DAOREF010000000
VERSION     DAOREF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28888_length_2569_cov_4.171042"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PKI33_09605"
     CDS             complement(<1..987)
                     /locus_tag="PKI33_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM37309.1"
                     /translation="MAKVVVIGAGFAGHTAALYLGRRLGKKHEITVISNRDVFGYVPS
                     WVWVGVGHMKPEQTIFKLKPVYDKMNVKCVYAAAKEIHPDEGDQYVLGEEVGSGKAVR
                     LNYDYLVIAPGPLLNFAGTPGLGPETGFTHSICSLPHAMKARDAYLALCERMKKGEKV
                     KIVIGTGHPGATCQGAAFEYIVNVHKDLVKKGLRDNADILWLSNEPAMGDFGVNGVQI
                     KRNGHVMKSEEFLTSVFNDHGIRYQVQTGVTKVEDGKIHWQNYEGEEGTTEFDFSMLI
                     PQFKGQPFKYIDKNGEDIPPKLTNPAGFVLVDGKYGLPYPELAQTPDAWPSQY"
     gene            complement(1016..1144)
                     /locus_tag="PKI33_09610"
     CDS             complement(1016..1144)
                     /locus_tag="PKI33_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37310.1"
                     /translation="MNGVIHLGSFSGISGTFGTVLYDVSATVFVYKTDGEVLRYLS"
     gene            complement(1277..1708)
                     /locus_tag="PKI33_09615"
     CDS             complement(1277..1708)
                     /locus_tag="PKI33_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNM37311.1"
                     /translation="MKRTSVLLLLLVLALALTACGRKPPAPLGADAPADNVPEIIGEY
                     GLNGEDATHEQYGGSLFITAGSQPGEYNLQWLVSGDIQEGIGTLAGNQLTFTWKSVEG
                     TDQSLSGYGTYTITVNGELYGERFVDGVAEPGIESVYPNPK"
     gene            complement(1761..>2569)
                     /locus_tag="PKI33_09620"
     CDS             complement(1761..>2569)
                     /locus_tag="PKI33_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNM37312.1"
                     /translation="SQVKAEANTFTFMPNASHIRFVIPLLGVMAVVTLAQIWLSNRPV
                     TFFTDDFATLAIQMTHHSGYAIHDITPPVDIEPDLTQPIRITLEVDHQPFFDETYTAT
                     DNHINQGARIFEQIQLPVGRHHITIKMYDRADASYEQILFDDTVNLEPRQSLTINFRD
                     VHLPDPKMGESIYYETASGVNAGCRICHSLTKGEKIIGPSFYGIADRAGERVPGLTAE
                     EYLHQSIVEPNAFVVPGYPSGQMIQNFGQILTEEQIQDLIAFLMTQKEDK"
BASE COUNT          602 a          612 c          695 g          660 t
ORIGIN      
        1 atactgggag ggccaggcat ctggggtttg agcaagctcg gggtaaggaa gaccatattt
       61 tccatccacc aacacaaaac cggcggggtt ggttagtttg ggggggatat cttcgccgtt
      121 cttatcgatg tatttgaagg gctgcccttt gaattgcgga atgagcatcg agaagtcaaa
      181 ttcagtggtg ccttcttcgc cttcgtagtt ttgccagtgg atcttgccat cttcgacctt
      241 ggtgacgccg gtttgaactt ggtagcgaat gccgtgatcg ttgaagaccg aggtgaggaa
      301 ttcttcgctc ttcatcacat gaccattgcg tttgatctga accccgttga cgccgaagtc
      361 gcccatggca ggttcgttcg agagccagag gatatcggca ttgtcgcgca gtcccttttt
      421 gacaaggtct ttgtgaacat tgacgatgta ctcaaacgcc gcaccctgac acgtcgcgcc
      481 ggggtgaccg gtaccgatga caatcttaac cttctcgcct ttcttcatgc gttcgcacaa
      541 agctaggtaa gcatcgcgcg ctttcatggc gtgaggcaga gaacaaatag agtgggtgaa
      601 tccggtctcg ggtccgagac cgggcgtgcc ggcaaagttg agtaaaggtc cgggagcgat
      661 gacgaggtaa tcatagttca ggcgcaccgc tttgcccgag ccgacttctt ctcccagcac
      721 gtattggtcg ccttcatcgg ggtggatttc tttggcggcg gcatacacgc acttgacatt
      781 cattttgtcg tacacgggtt tgagtttgaa gatggtctgc tcgggtttca tatgcccaac
      841 gccgacccaa acccatgaag ggacgtagcc aaacacatcc cgattactaa tcacggtgat
      901 ctcgtgtttt ttaccgagac gacgccccag atacagtgcc gcggtatgac cagcgaaccc
      961 tgctccgatc actacaactt ttgccatatt ctttctctcc tttgggaatt aagaatcaag
     1021 acagatagcg aaggacttcg ccatctgtct tgtagacgaa aactgttgca gacacatcat
     1081 agagtactgt gccaaatgtg cctgaaatgc ctgaaaacga gccaaggtga attactccat
     1141 tcatctgaat acatgatgtg gacttcacga cggtcatcga acaataaagc gatgcgcgtc
     1201 tctcgatatt gggctgacag ttcttattaa agttgtttta tagaagactt gctacgagca
     1261 gagtgatgaa aactgattat tttggatttg ggtataccga ctcgatgccg ggttcagcga
     1321 caccgtctac aaaacgctca ccgtagagtt ctccgttcac ggtgatggtg taagtaccgt
     1381 aaccgctcag agattggtct gtgccttcca cgctcttcca ggtgaaggtt agttgattgc
     1441 ctgcaagcgt gccaatccct tcttgaatat cgccagagac tagccattga aggttgtatt
     1501 cacccggctg gctgcccgct gtgataaata acgagccgcc atattgttca tgggtggcat
     1561 cttcaccatt caacccgtat tcaccaatga tttcgggcac attatctgcg ggggcatctg
     1621 cgccgagagg ggcagggggc ttgcgtccgc aggcggtgag ggcgagagca aggacaagta
     1681 ataaaagtag cacgctggtt cgtttcatag ttcctcaact tagatgacga tcaccctaaa
     1741 aagtgacggt caattagatt ttatttatcc tctttttggg tcatcaagaa agcgatcagg
     1801 tcttgaatct gttcttcggt gaggatctgc ccgaagtttt gaatcatctg tccactgggg
     1861 tagccaggca ccacaaaagc attcggctcc acaatagatt gatgcagata ctcttcggca
     1921 gttaagccgg gcacacgttc accggcacgg tctgcaatac cgtagaaaga cggaccgatg
     1981 atcttttcgc ctttggtcag tgagtgacaa atgcggcaac cagcattgac cccagaagcg
     2041 gtttcgtaat aaatcgattc gcccatcttc gggtctggca agtgaacgtc tctgaagttg
     2101 atggtcagcg attggcgcgg ctctaggttg acggtatcgt caaagaggat ttgctcatag
     2161 ctggcatcgg cacggtcata catcttgatg gtgatgtgat gtctgccaac gggcagttga
     2221 atctgctcga agatgcgtgc gccttggttg atgtgattat cggtagcggt ataggtttcg
     2281 tcgaagaagg gttggtgatc cacttccagc gtaatgcgaa tcggttgggt cagatcaggc
     2341 tcaatgtcca ccggaggcgt aatgtcatga atggcgtagc cgctgtgatg agtcatctgg
     2401 atcgccagtg tggcaaagtc atctgtaaag aacgtcactg ggcgattgct gagccatatc
     2461 tgtgccaagg tgacgaccgc catcacaccg agcaatggga tgacgaaacg aatgtggctg
     2521 gcattcggca tgaaggtgaa ggtgttcgct tcagccttaa cctgagact
//
LOCUS       DAOREF010000442         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_28995_length_2564_cov_3.630530, whole genome shotgun sequence.
ACCESSION   DAOREF010000442 DAOREF010000000
VERSION     DAOREF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28995_length_2564_cov_3.630530"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKI33_09625"
     CDS             complement(<1..994)
                     /locus_tag="PKI33_09625"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNM37313.1"
                     /translation="MAKNIEDYYAPWVKGIPMYISEHIELAWRKPELHRMMSNENPLP
                     PSEKVLEAMFKYAKMTNRYPDQGLVVRQKIAEINGVAGPQNVMIGNGSSEVYDNIFRM
                     FLEPGDEVIQHTPCFGIYGLRGTLLGAKMVSVPMIYKDHKLIFDHEGLMNAVTDKTKI
                     IVIPNPNNPSGNFMDPQHFEPFAKLGIPLVVDEAYIEYAGLGMSQVELTKKYKNVFIT
                     RTLSKAYGLAGMRFGYTIAHEDTLKQVAGSLLPWNVGTIPMWAALAAFEDTEGLAERV
                     KFNNDAVDYISEALSVIPGFYVMQSKANYILFDCGETGKTGKQVLEYAETKGIIL"
     gene            complement(1076..1951)
                     /locus_tag="PKI33_09630"
     CDS             complement(1076..1951)
                     /locus_tag="PKI33_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HNM37314.1"
                     /translation="MSIVNAKEIMIPAAKEGWAVGAFNVTDLLQFEAVIDAAIEKKAP
                     VIVQTSVKPSQFLGTNMMVAIYRTLAESAPVPVCLHLDHSTDINYCKKCADAGYTNIM
                     IDASKQSYEENIRQTKEVVDYAHSVGNISVEGELGTVGGVEDQIKVAEDEAQLANPEQ
                     SIEFVERTGVDIFAPAIGTAHGVYKTKNPKIDFERMATIHKMLNSNGIKTPLVVHGGT
                     GLPDDYVAKLLAAGGAKFNVSTELKHTLINAKWEYLSAHRDEYDPGKLDVFVRDATRK
                     AVMHWMDKLGCNGKA"
     gene            complement(2066..>2564)
                     /locus_tag="PKI33_09635"
     CDS             complement(2066..>2564)
                     /locus_tag="PKI33_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNM37315.1"
                     /translation="AKGAHFPEDWAFIQKKMREAEKALLKDPYKPTPCHNDLLNLNWL
                     DEEVAGDIGEIRLLDWEYAGMGDIFFDLGNFSHHHRLNEDQIRIFLTAYFGKFTPKHY
                     ARLRIMWAMSELHEAMWGTTQSVISTLEEDFQGYANLWFGRYRMHVTDYRWEQWLKDA
                     SKKGK"
BASE COUNT          572 a          649 c          653 g          690 t
ORIGIN      
        1 gcaggatgat gcccttggtt tcagcgtatt cgagcacctg cttgccggtc ttgccggttt
       61 cgccgcagtc gaacagaatg tagttggctt tggactgcat gacatagaag ccggggatga
      121 cgctcaaggc ttcgctgatg taatccaccg catcattgtt gaacttgacg cgttccgcca
      181 gaccttcggt atcttcgaac gctgcgagtg cagcccacat ggggatggta cctacgttcc
      241 aaggaaggag agagccagca acctgtttga gggtgtcttc atgggcaatg gtgtaaccaa
      301 aacgcatacc agccagacca tacgccttgg agagtgtgcg ggtgatgaac acgttcttgt
      361 acttcttggt cagctcaacc tgtgacatgc ccaaaccggc atattcaatg taagcttcat
      421 ccacgaccaa tgggataccc aacttggcaa acggttcgaa gtgttgcggg tccatgaagt
      481 tgccgctggg gttgttgggg ttcgggatga cgatgatctt ggtcttatcg gttactgcgt
      541 tcatcagacc ttcatggtcg aagatgagct tgtgatcttt atagatcatc ggcacagaga
      601 ccattttggc gccgagcaag gttccgcgca gaccgtaaat gccgaagcag ggtgtgtgct
      661 gaatgacttc atcgcccggt tcgaggaaca tgcggaagat gttgtcatac acttcgctgg
      721 agccgttgcc aatcatcacg ttctgcggac cggcaacccc gttgatctca gcaattttct
      781 gacgtaccac caaaccctga tcgggatagc gattggtcat cttggcgtac ttgaacatcg
      841 cctctagcac cttttcagaa gggggcagcg gattttcatt ggacatcatg cgatgcagtt
      901 cgggtttgcg ccaagccaac tcaatgtgct ccgaaatgta catcggaatg cccttcaccc
      961 acggggcgta ataatcttcg atatttttag ccatgttact tgttctccta aattattttt
     1021 acgaattatc acgatggacg atcttatctg ccggacatcg ttgattgtct tatgattaag
     1081 ccttgccgtt acagccgagc ttgtccatcc agtgcattac ggctttgcgg gtcgcatcgc
     1141 ggacgaagac atcgagcttg cctgggtcgt attcatcacg gtgggcgctg agatattccc
     1201 atttggcgtt gatgagggtg tgcttcaact cggtggagac gttgaacttc gcaccgccag
     1261 ccgccaacaa cttggcaaca taatcatcgg gcaagccggt tccgccgtgg acgacgagcg
     1321 gggtcttgat gccattgctg ttcagcatct tgtgaatggt cgccatgcgt tcgaaatcga
     1381 tcttgggatt cttggtcttg tacacgccgt gtgcggtgcc gatggcggga gcgaaaatgt
     1441 ccacacccgt gcgttcgacg aattcaatgg attgttcggg gttggcgagt tgtgcttcat
     1501 cttccgcgac cttgatctgg tcttccacac caccaacggt tccgagttcg ccttccacag
     1561 aaatgttgcc aacgctgtga gcataatcca ccacttcttt ggtctggcgg atgttctctt
     1621 cataggactg ttttgatgcg tcgatcatga tgttggtata cccagcatcc gcgcacttct
     1681 tgcagtagtt gatatcggtg ctgtgatcga ggtgcaaaca caccggcaca ggcgcagatt
     1741 cagccaatgt gcggtagatc gctaccatca tattggtgcc gaggaactgt gaaggcttca
     1801 cagaagtctg cacaatgaca ggggctttct tttcaattgc cgcgtcgatc accgcttcaa
     1861 attgcagcaa gtcggtgacg ttgaacgcgc ccacagccca accttctttt gccgcaggga
     1921 tcataatctc ttttgcattt acgatagaca tttggtactc cttggttttt tcttttcagg
     1981 tttcgagttt gtatcgaaag aggaaaaatg aaaataaact atcttctttc ctctttcgcc
     2041 aattaccaac tacttatgaa ctaacttact tgcccttctt cgacgcatct ttcaaccact
     2101 gttcccagcg atagtcagtg acatgcatgc gatagcgccc gaaccacaaa ttggcatagc
     2161 cttggaaatc ttcttccaac gtggagatga ccgattgggt tgtgccccac atggcttcgt
     2221 gcaactcaga catcgcccac ataattctca gccgcgcata atgcttgggg gtgaacttcc
     2281 caaaataagc ggtaaggaag atgcggattt gatcctcgtt caaacgatgg tggtgcgaga
     2341 aattcccgag gtcaaagaaa atatcgccca tgcctgcata ttcccaatcg agcaagcgga
     2401 tctcgccaat atcgcccgca acttcttcat ccaaccagtt gaggttgagc aaatcgttgt
     2461 ggcagggagt gggtttgtag gggtctttca acaatgcctt ttctgcttcg cgcattttct
     2521 tttgaatgaa cgcccaatct tcaggaaagt gcgcgccctt ggct
//
LOCUS       DAOREF010000443         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29036_length_2562_cov_3.825289, whole genome shotgun sequence.
ACCESSION   DAOREF010000443 DAOREF010000000
VERSION     DAOREF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29036_length_2562_cov_3.825289"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>545)
                     /locus_tag="PKI33_09640"
     CDS             complement(<1..>545)
                     /locus_tag="PKI33_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNM37316.1"
                     /translation="TMGGIIVDSGKFNWSKSGRFKNFTEPDPAYHHVIYADDFGPLAF
                     ISKARAGILRDLGTVQQPIASWFLIQGLETLSLRMTRHVQNAKRAAEFLENHPNVKWV
                     SYAGLPSHPDHERAQKYLPQGTGAVLGFGVKGGREAGAKFIDNLQLFSHVANIGDTRS
                     LAIHPASTTHSQLSDEEQTAA"
     assembly_gap    546..559
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<560..1200)
                     /locus_tag="PKI33_09645"
     CDS             complement(<560..1200)
                     /locus_tag="PKI33_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM37317.1"
                     /translation="MTSKHNFGFTTRQIHEGYTPDPATGSRAVPIYQTTAYDLQSTDR
                     AARLFALQENGNIYTRISNPTSNVFEKRVASLEGGIGALEVGSGHAAQATAILTICEA
                     GDHIVSTSLLYGGTVTQFKYTFPKLGITVTLVDPSDPENFRRAIQPNTKIIYGETISN
                     PLGNIFPFEEVSKIAHEHGIPLMIDNTFATPYLCRPFEWGADIVTHSTTKFIGG"
     gene            complement(1200..2126)
                     /gene="metA"
                     /locus_tag="PKI33_09650"
     CDS             complement(1200..2126)
                     /gene="metA"
                     /locus_tag="PKI33_09650"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNM37318.1"
                     /translation="MPVKIPSTLPAREILEYENIFVIDEERALHQDIRALRVAILNLM
                     PTKVATETQILRLLSNSPLQVEITLVHTATHESKNTDAEHLLQHYVTFEDIKHEKFDG
                     LIITGAPVEQLPFEEVDYWEELKQVMDWAKTNTESTFYICWGAQAGLYHYYGVPKYDL
                     PQKMFGVFEHRLLNRTESLMRGFDDIFLAPHSRHTEIRREDIEKVPELQILAESDDAG
                     VYIVGSRDGRELFITGHSEYDPLTLKAEYDRDVNKGLPIHVPKNYYPNDDPKQMPNVR
                     WRGHANLLYANWLNYYVYQATPFDPNEIPKRN"
     gene            complement(2200..>2562)
                     /locus_tag="PKI33_09655"
     CDS             complement(2200..>2562)
                     /locus_tag="PKI33_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNM37319.1"
                     /translation="EFLEKHPKVSWVKYPGLKSHPDYDRAKKYLPKGAGAILGFGIKG
                     GAAAGKKFIESLQLFSHLANVGDARSLAIHPATTTHSQLTEEQQVTAGVSPDFVRLSI
                     GLEDFEDIKWDLDQALNA"
BASE COUNT          566 a          543 c          730 g          709 t
ORIGIN      
        1 ctgcggcggt ttgctcttcg tcggagagtt ggctgtgggt ggtgctggcg ggatgaattg
       61 ccagcgagcg cgtgtcacca atgttggcga cgtggctgaa gagttgcagg ttatcgatga
      121 acttcgctcc tgcttcacgc ccgcctttga ctccaaaccc gagtacagcg cctgtgcctt
      181 ggggtaaata tttctgggcg cgttcatggt ctgggtgact gggtaaacct gcgtatgaaa
      241 cccatttcac attgggatga ttctcaagaa actcggcggc tcgtttcgca ttttgcacgt
      301 gacgtgtcat tcgcaacgat aaggtttcta acccttgaat aagaaaccag gatgcaattg
      361 gctgttggac ggtgcctaag tctcgtaaga taccagcgcg tgctttggag atgaacgcca
      421 atggaccaaa gtcatcggcg tagataacgt gatggtatgc aggatcaggc tcggtgaagt
      481 ttttgaagcg cccgctctta ctccaattga atttgccact atctacgata attccgccca
      541 tggtgnnnnn nnnnnnnnnc cgccgatgaa tttggtggtg gagtgggtga cgatatctgc
      601 accccattcg aagggacgac acaagtaggg cgtggcgaag gtattatcga tcatgagcgg
      661 gatgccatgt tcgtgtgcga tcttcgagac ttcttcgaat ggaaagatat tgccaagcgg
      721 gttgctgatg gtttcgccgt agattatttt tgtatttggc tgaatggcgc gacgaaagtt
      781 ttccgggtcg cttgggtcta cgagagtgac ggtgattcca agttttggga atgtgtattt
      841 gaattgggtg actgttccgc catacagcag ggaggtggag acgatatggt caccggcttc
      901 gcaaatagtg aggatggcgg tagcttgcgc ggcgtgacca gagccaactt caagcgcgcc
      961 gattccgccc tcgaggctgg cgacgcgctt ctcgaagacg ttgctggttg ggtttgagat
     1021 gcgagtgtag atattaccgt tttcttgaag ggcaaagagt cttgcggcac ggtctgtgct
     1081 ttgcaggtcg taggctgtgg tttggtagat cggaacagcc cgactaccgg tagcggggtc
     1141 tggagtgtag ccttcgtgga tttgcctagt ggtgaagccg aagttatgtt ttgaagtcat
     1201 tagttccgct ttgggatttc atttgggtca aagggagttg cttggtatac gtaatagttc
     1261 aaccagttgg cgtacagtaa attcgcatgc ccgcgccagc gcacgtttgg catttgcttg
     1321 gggtcatcat ttggataata atttttcgga acgtgaatag gtaacccctt gttcacatca
     1381 cggtcgtatt ctgctttaag tgtgagaggg tcgtattcag agtgacccgt aataaagagc
     1441 tcacgtccat ctcttgaacc gacgatgtaa acacctgcat catctgattc ggcaaggatt
     1501 tgtaattcag gcaccttttc aatatcttcg cggcgaatct cagtatgacg ggagtgtgga
     1561 gcgagaaaaa tgtcgtcaaa gccgcgcatc aggctttcgg tccggtttaa gaggcggtgt
     1621 tcaaaaacgc cgaacatttt ttgcggcagg tcatatttgg ggacgccgta gtaatgatag
     1681 agtccggctt gggctcccca acaaatataa aatgtggact cggtattggt ttttgcccaa
     1741 tccatcacct gtttgagttc ttcccagtaa tctacttctt caaaggggag ttgttccact
     1801 ggcgcacccg tgataatgag cccatcgaat ttttcatgct tgatatcttc aaaggtgaca
     1861 taatgttgca gcagatgttc tgcatctgtg ttcttggatt cgtgggtagc ggtgtgaacc
     1921 agtgtgatct ccacttgcaa cggagagttt gaaagtaagc gcagaatctg tgtttctgta
     1981 gcgaccttgg ttggcattag attcagaatt gccacacgca aggcacggat gtcttgatgt
     2041 aaggcgcgct cttcgtcgat gacgaagata ttctcgtatt cgagaatttc acgagcagga
     2101 agagtagaag ggatttttac aggcatgagt ttgaaatgat gatttatgca tgtggaatga
     2161 agaatttttt tttcttcatt ccacatgcaa tttctatgtt taggcgttaa gcgcctgatc
     2221 gagatcccac ttgatgtctt cgaaatcttc gagaccaatg ctcaagcgga cgaaatcagg
     2281 actgacgcca gcggtgacct gttgttcttc ggtcaactga ctgtgagtgg tggtagcggg
     2341 atgaatggcg agagagcggg catcaccaac gttggcaagg tggctgaaga gctgcaggct
     2401 ttcaatgaac ttcttgcccg cggctgcacc gcccttgata ccgaacccga gaatcgcacc
     2461 tgcgcctttg ggcagatact tcttggcgcg gtcgtagtct gggtggctct tgagcccagg
     2521 atatttgacc cagctgactt taggatgttt ctcaaggaat tc
//
LOCUS       DAOREF010000444         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29072_length_2560_cov_4.068663, whole genome shotgun sequence.
ACCESSION   DAOREF010000444 DAOREF010000000
VERSION     DAOREF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29072_length_2560_cov_4.068663"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /gene="amrB"
                     /locus_tag="PKI33_09660"
     CDS             <1..539
                     /gene="amrB"
                     /locus_tag="PKI33_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNM37320.1"
                     /translation="TPLGNIEVDQHIINELQSYLKIPITSIANDKEHSLEIELPFLQQ
                     VLKNDFKLLPIMIRAQEKSVAKELGEALAKTIKKRNAILIASTDLSHFYDQQTAQKLD
                     QEMLNRFTTLNPTTIFEAEQTGKGFACGHAAVATAQWAAKELGANNVQILKYATSGDI
                     TGDFQSVVGYGAAAILRT"
     gene            complement(582..1019)
                     /locus_tag="PKI33_09665"
     CDS             complement(582..1019)
                     /locus_tag="PKI33_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37321.1"
                     /translation="MSIVILVAGALMILIALGLLAMVFMKSNQVNLTEKTDGKPEWMH
                     AMPPKETVEATMKDGEGVTLYNYDEGEKVATPFAEQIEDVLRAKVESDPYLKSFTIDF
                     GTAADGGLEIWVNGEKYDGVANLPDEQLKQSLLEAVKDWNSRK"
     gene            1055..>2560
                     /gene="priA"
                     /locus_tag="PKI33_09670"
     CDS             1055..>2560
                     /gene="priA"
                     /locus_tag="PKI33_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNM37322.1"
                     /translation="MFARLAVNISAISGVFDYSIPAELVPQVRAGCLVTASFGNQTVQ
                     GIIVELTDSSSVPNPKQILDLLDPAPLLTPPQLALAIHLADSTLNPLSAIIGMMIPTG
                     LSQQADTLYAVRTPEYSDPSAKVVTRILNLLHERGPLRGRQIDSHFAKIDWRKSANML
                     VKKGVLTSKNVLPPPRVRPKNIRVAQLAVTPEAAEEMMASLGSTKATQTRRESALRFL
                     IQQPEPIHLSWVYAQTGCNISDLQELEERGLIRLFENEIFRDPLERIEKQETKSELSL
                     TSEQNSALQAILSSFRSQPSGFLLYGVTGSGKTEIYLRAAQEVIKSGRQAIILVPEIA
                     LTPQAVRRFVSRFPGQVGLVHSKLSEGERYDTWRRARDGKLNVIIGARSALFAPLPNI
                     GLIVVDECHDSSYYQSEPPYYNAVTAAQEYAKLCGAVCVLGSATPTIEQRFLAEQNQI
                     TRLDLPNRVTESTLPPVQVVDMREELKNGNRGIFSKSLAESLAEVLERKEQA"
BASE COUNT          672 a          693 c          534 g          661 t
ORIGIN      
        1 ccactccgct cggcaatatt gaagtagatc aacatataat taacgaactt caatcttact
       61 taaaaattcc cattacatct atcgccaacg ataaagaaca ctcgcttgag attgagctcc
      121 cttttttgca acaagtcctt aaaaacgatt tcaaactttt acccatcatg attcgcgcac
      181 aagaaaaatc tgtcgccaaa gaactggggg aagcactggc aaagaccatc aaaaaacgca
      241 atgccattct aattgcctcc acagaccttt ctcattttta cgaccagcaa acggctcaaa
      301 aacttgacca ggaaatgcta aatcgcttta ctacgcttaa tcccactaca attttcgaag
      361 cagaacaaac agggaaaggc tttgcttgcg gtcatgccgc tgttgcaact gcgcaatggg
      421 cagccaaaga acttggtgcg aacaatgttc aaatcttgaa gtatgcgaca tccggcgata
      481 tcactggaga ttttcagtct gttgttggct atggagccgc agcaatcttg cgtacctaac
      541 aaaaagcgcc agagaaatct ctggcgcttt tttggacttt tctattttct actgttccaa
      601 tcttttactg cctcaagcaa agattgctta agctgttcat cgggcaagtt ggcaacccca
      661 tcatattttt caccatttac ccaaatctcc aagccgccat cagcagccgt gccaaaatca
      721 atcgtgaacg attttaagta cgggtcgctt tccacttttg cacgcagtac atcttcgatc
      781 tgttcggcaa atggagtagc aaccttttca ccttcatcat agttatatag cgttacacct
      841 tcgccatctt tcatcgttgc ttccaccgtc tctttgggcg gcatggcatg catccattca
      901 ggtttgccgt cggtcttttc cgtcaaattt acctgattcg atttcataaa caccatcgcc
      961 aacagcccca atgcgatcaa aatcatcaaa gcgccagcaa caagaattac aatgctcata
     1021 ggtcagcctc cattgaaaca attataatcg tctcatgttt gcccgtcttg ccgtcaatat
     1081 ctcagccatc tctggcgtgt tcgattacag catccctgca gagcttgttc cacaggttcg
     1141 ggcggggtgt cttgttacag catcatttgg aaatcagact gtgcagggca ttatcgtcga
     1201 gttgaccgat tcttcttcgg taccgaatcc taaacagatc cttgacctgc tagaccctgc
     1261 cccgcttctc accccgcctc agcttgctct tgccattcat ctagcagact ctacactgaa
     1321 tccgctctct gccatcattg gcatgatgat ccccaccggg ctcagccagc aagcagatac
     1381 tctttacgca gtacgaactc ctgaatattc agacccatct gcaaaagttg tcacccgcat
     1441 ccttaaccta ctccacgaac gcggaccatt acgcggacgc caaatcgatt ctcatttcgc
     1501 aaaaatagac tggcgcaagt ctgccaacat gctcgtcaaa aaaggtgtgc taacctcaaa
     1561 gaatgtcttg cctccgccca gagtgagacc caagaatatt cgcgttgccc aactcgcagt
     1621 cacgccagag gcggcagaag aaatgatggc atcgcttggt tccacgaaag cgactcagac
     1681 tcgacgcgaa tccgctctac gcttcctcat ccaacagccc gaaccgattc atctttcatg
     1741 ggtgtatgcg caaacaggct gcaatatttc agacttgcaa gagcttgaag aacgcggtct
     1801 gattcgcctg tttgaaaatg aaatctttcg cgacccgttg gagagaattg agaaacaaga
     1861 aacaaagagt gagttatcgc ttacatcaga acaaaattct gctttgcaag ccattctttc
     1921 atccttccgc tctcaacctt ctgggtttct tttatacggc gtgaccggtt ctggcaaaac
     1981 ggaaatttac ttacgcgccg cacaagaagt gatcaaaagc ggaagacaag ccatcattct
     2041 tgtgcctgaa attgcgctca ccccgcaagc tgtgcggcgc tttgtctcac gttttccggg
     2101 tcaagtcggt ttggttcact cgaaactttc tgaaggggaa cgatacgata catggcgtcg
     2161 tgcccgcgat gggaagttga acgtcattat tggggctcgt tcggcgttgt ttgcgccttt
     2221 gccaaatatt ggtctcatcg tcgtggatga atgccatgac agttcatact atcaaagcga
     2281 accgccttat tacaacgccg tgaccgccgc acaggaatat gcaaaacttt gtggcgcggt
     2341 ttgtgtgctg ggctctgcca cgcccacgat tgagcagcgt ttccttgcgg agcaaaacca
     2401 gatcacacgg ctcgacttgc ccaaccgtgt taccgagtct acgttgccgc cagtgcaagt
     2461 agtggacatg cgtgaggagt tgaagaacgg taaccgtggc attttctcga aatcattagc
     2521 cgagtcacta gccgaagtct tggagcgcaa agaacaagcc
//
LOCUS       DAOREF010000445         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29094_length_2559_cov_2.970447, whole genome shotgun sequence.
ACCESSION   DAOREF010000445 DAOREF010000000
VERSION     DAOREF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29094_length_2559_cov_2.970447"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKI33_09675"
     CDS             complement(<1..684)
                     /locus_tag="PKI33_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37323.1"
                     /translation="MQNEFKEDAPTPSGKPTSQRAGGTSNGPSLFTRLINSLVNMGLG
                     EPLIKLGVNLFSVVAIVLVVFMSRSFYRQAQDMPVAPNQDSGPDIEAAPAESLAVLDI
                     PVTDGINRAAQLHTTIPSRPRNEVSTYVVQDGDTVFGIAEKFGLKPQTILWGNYNTLL
                     DDPHSLKPGQELTILPVDGVYWEWLGGIPFGEWAKFYGVNAADIIEYPGNNIDPTTVG
                     DYENANIKAG"
     gene            complement(749..2020)
                     /locus_tag="PKI33_09680"
     CDS             complement(749..2020)
                     /locus_tag="PKI33_09680"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37324.1"
                     /translation="MVSLKNWLITRFPALASRDYVLFVVGQFISVIGTWMQATALPYL
                     AYRISGRPLDLGLIGFSNTLPTLLFALPAGVLVEHLDKRKAVILLQAVMSVQAFGLTY
                     LAFTNKLEIWHILVLAFIYGSAVSVEVTARQAMLIELAGKEALPSAIALQTTAFNLGR
                     VIGPLLAAWLIAISGNEGTVFLANGVSYVFVIIGLFFARTRYRVEPEAKTSQGMGSEF
                     KEGVRYIRSNTIVFSAILMSALLGFFGIPLVQQLPAIARDILQPLLTSEAAIATRTSN
                     LYTAQGIGAVTAAFMMAYFATANKNSTLLWGQILFIFPIIVLGLTTNASLAFFLLIFI
                     GWGTVVQLISMNTMIQMRVPNDLRGRVFSIYFWGLQGVAPFGSILIGWIAQTWDVQFA
                     IVAGGIACLVGISVVRLLFFRKNEPTEQISY"
     gene            complement(2024..>2559)
                     /locus_tag="PKI33_09685"
     CDS             complement(2024..>2559)
                     /locus_tag="PKI33_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNM37325.1"
                     /translation="KNKADFFLWGLIPSWSKDPSIGNKLINARGETLAEKPSFRGSFK
                     YKRCLIPVDGFYEWKSHPGSTSKTPYFIHMKDRKPFAFAGLWDEWQSPDGDSVRTCTI
                     ITTDPNELMSTLHSRMPVILHPDDYALWLDPAPQTPEKLMHLIKPFPTENMSAYPVST
                     LVNKPGNDRPECVVPAS"
BASE COUNT          703 a          565 c          664 g          627 t
ORIGIN      
        1 cccagctttg atgttcgcat tttcataatc accaaccgtg gttgggtcta tattgttgcc
       61 gggatattcg atgatatctg cggcatttac accataaaac tttgcccatt cgccaaaggg
      121 aattccgccc agccattccc aatacacacc gtccaccgga agaatggtta actcttgacc
      181 cggtttgagt gagtggggat catctagcaa cgtgttgtaa ttgccccata aaattgtttg
      241 cggtttcaaa ccaaattttt ctgcaatacc aaaaacagta tcaccatctt gcacaacata
      301 agtggaaact tcattgcgcg ggcggcttgg aatggtagta tgcaattgag ctgcgcgatt
      361 gatcccatcg gtgacgggga tatcaagcac tgcaagagac tctgcgggcg ccgcctcaat
      421 atctggacct gagtcctgat tcggcgctac gggcatatct tgcgcctgac ggtaaaacga
      481 ccgagacata aagaccacca acacaatggc aacaactgaa aagagattta ccccaagttt
      541 aattaatggc tcgcctaacc ccatattcac aaggctgttt ataagtcttg taaatagtga
      601 gggaccattt gaggttcccc cagcgcgttg agaggttggt tttcccgagg gggtaggtgc
      661 atcttcttta aattcgtttt gcatagggtt catgataaca tggcaaaaat ttcacagtca
      721 agttggggtg taaaaccctc atacagcctt aataagaaat ttgctctgta ggctcatttt
      781 ttcgaaaaaa tagcaatcgc acgactgaaa tgccaactag gcaggcaata ccgcccgcca
      841 caattgcaaa ttgtacatcc catgtttgag cgatccaacc gataagtatg ctcccgaacg
      901 gagcgacgcc ctgtagtccc caaaagtaaa tgctaaagac acgtccgcgc agatcgttgg
      961 gaacgcgcat ttgaatcatg gtattcatgc tgataagctg gacgaccgtg ccccaaccaa
     1021 tgaagatcaa caaaaagaat gccagagagg cgtttgtggt taatcctaat acgataatgg
     1081 ggaaaataaa aaggatttgc ccccacagta atgtggagtt tttatttgca gtcgcaaaat
     1141 acgccatcat aaatgcggca gtcacggctc cgatgccttg agcggtgtag agattgctgg
     1201 tgcgggttgc gatggcggct tcggaagtga gaagaggctg caaaatatca cgtgcaatag
     1261 cagggagttg ttgtacgagg ggaataccga aaaagccaag caatgccgac atcaaaatgg
     1321 cggagaatac gatggtgttt gatcgaatat aacgtacacc ttctttgaat tcactgccca
     1381 tgccttgaga tgtttttgct tctggttcaa ccctgtatcg tgttcgcgca aagaacagac
     1441 cgatgataac gaacacataa ctgacaccat ttgcgagaaa gaccgtccct tcgttgccag
     1501 agatggcaat caaccaagcg gctagtaacg gtccaataac acgcccaagg ttgaaggcgg
     1561 tggtttgcag ggcgatggca cttggcaggg cttcttttcc tgcgagttcg atcaacatgg
     1621 cttggcgtgc agttacttca acggagactg ctgaaccgta gataaacgcc aacactaaaa
     1681 tatgccaaat ttcgagttta ttggtaaagg caagataggt cagaccgaat gcttgcacag
     1741 acatcactgc ttgcaggagg attactgctt tacgtttgtc taaatgttca accagtacgc
     1801 ccgcgggcaa ggcaaaaagc agagtaggta atgtgttgga gaaaccgatc aaccccaagt
     1861 ctaagggacg acccgagata cgataggcaa gatatggcaa ggcggtagct tgcatccatg
     1921 tgccgataac ggagataaat tgacctacga cgaatagaac gtaatcacgt gaagcgagag
     1981 cggggaagcg ggtaatgagc cagtttttga gggataccat tttctacgat gcgggaacga
     2041 cacactcggg gcggtcattc cctggtttgt tgaccaaggt tgagacggga taggcggaca
     2101 tattttcggt agggaaaggc ttgatgaggt gcatcagctt ttcgggagtc tgaggcgcgg
     2161 gatctaacca gagggcgtaa tcatcggggt gaagaatcac aggcattcgg ctgtgcagag
     2221 tcgacatcaa ctcgttgggg tcggtggtga tgatggtgca agtacgtaca gagtcgccat
     2281 ctggtgattg ccattcgtcc cataacccgg cgaaggcaaa tggctttcga tctttcatgt
     2341 ggatgaagta cggggtcttt gaggtgctgc cagggtgaga tttccattca tagaatccat
     2401 ctacagggat aaggcagcgt ttgtatttga agctgccgcg aaacgatggc ttttctgcca
     2461 gagtctcgcc gcgggcattg atgagtttgt tgcctatgct tgggtctttt gaccaagatg
     2521 gaatcaatcc ccataaaaag aaatcggctt tgttttttg
//
LOCUS       DAOREF010000446         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29129_length_2557_cov_4.399281, whole genome shotgun sequence.
ACCESSION   DAOREF010000446 DAOREF010000000
VERSION     DAOREF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29129_length_2557_cov_4.399281"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="PKI33_09690"
     CDS             complement(<1..1490)
                     /locus_tag="PKI33_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNM37326.1"
                     /translation="MQKQSLTSLWQFRQLGSIEWLPAQVPGGVHTDLMALARIPDPFV
                     GDNEKRVAWVAESDWEYHTTFNVQADVFQRKNIWLVCDGLDTLASLTLNGKALGETNN
                     MFRQYRFDIKSLLKAEGNELTITFKSPVKFVTEKNTARPMNGVSQAIAGGPHLRKAPC
                     HFGWDWGPQLPPIGIWKDIYLEAYDSARISEVHLRQKHDNGKVTISARVEVENGNAPL
                     SATLLVTAPDGSEQAESVSFTSASGVVSVQVKNPQLWWPNGLGDQPLYQVRVLLSEGN
                     QTLDEKKYQMGLRKIELKQEPDEWGKSFTFVVNGHPFFAKGADWIPADSFPTRLTEEA
                     LEGLIRSAVATHQNMLRVWGGGFYEDERFYDLCDRYGILLWQDFIFSCSIYPLDEPEF
                     LENVRVEVVENIRRIRHRASLALWCGNNEMEQGWAEWGWQNPELDTLKAVYDKFFHHT
                     LKDWCNAEDPDNVYWPSSPSSDVPFEDPNGQIQGDAHYWEVWHRGKP"
     gene            complement(1540..2352)
                     /locus_tag="PKI33_09695"
     CDS             complement(1540..2352)
                     /locus_tag="PKI33_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM37327.1"
                     /translation="MPSEKKLYLSAKGITKTFGFGRQKTVAVDNVDLDLYEGEVVSIV
                     GESGSGKTTLSKVLLGLMNQTSGDVYFQGVKRDISTQSRKQEYWKNIQAIFQDPFSSY
                     NIFNKIDSVLLDCINMRGGKYLPREKKLEMMTEACSFVNLKFEELTNKYPFELSGGQM
                     QRLMIARIFLLQPKILLADEPTSMIDACSRATILDELMHLRKEIGMTIIFITHDIGLA
                     YYVSDSVYIMEKGKFVEGGSADEVILTPRHPYTKRLINDVPKIYEEWDLSTV"
     gene            complement(2342..>2557)
                     /locus_tag="PKI33_09700"
     CDS             complement(2342..>2557)
                     /locus_tag="PKI33_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37328.1"
                     /translation="LTAIPGVPPNLKKPPSGCRFAERCRFVRPECRVASVPLHEASNG
                     RMYRCIIPEAELRAIYAREQEEQKNAK"
BASE COUNT          621 a          627 c          624 g          685 t
ORIGIN      
        1 ggtttgcccc tgtgccatac ttcccaataa tgtgcatcac cttgaatttg tccgttcggg
       61 tcttcaaagg gaacatcaga ggaaggcgaa ctgggccagt acacattgtc ggggtcttcg
      121 gcattgcacc aatctttcaa ggtgtggtgg aagaacttgt cgtacacggc tttcaaggtg
      181 tcaagctctg ggttttgcca accccattca gcccagcctt gttccatttc gttgttgccg
      241 caccacaatg ccaaagaagc acggtgacga atgcggcgaa tattctccac aacttccact
      301 cgcacgttct ctaaaaattc aggctcgtcg agcgggtaaa tgctgcagga gaaaataaag
      361 tcctgccaaa gcaaaatgcc gtaacggtcg cacaaatcgt agaaacgctc atcttcataa
      421 aagccaccac cccacacccg cagcatattt tgatgcgtcg ccacggcaga acggatcaac
      481 ccttcaagcg cttcttccgt caaacgagtt gggaaggaat cagcaggaat ccaatctgcg
      541 cctttggcga agaagggatg tccattgact acaaaggtga aggacttgcc ccattcatcc
      601 ggttcttgct taagctcaat cttgcgcaaa cccatttggt atttcttttc gtccaaagtt
      661 tggttgccct cacttaagag cacacgtact tggtaaaggg gttggtctcc caaaccattg
      721 ggccaccaca actgcggatt cttcacttgc acagaaacaa ctccgctggc agaagtgaag
      781 gacacgcttt cggcttgctc tgaaccgtct ggagcggtaa caagtaaggt cgcagaaaga
      841 ggcgcgtttc cgttttccac ttccacccgc gcagaaattg ttaccttgcc attgtcatgt
      901 ttttggcgaa gatgaacttc agaaatacga gccgagtcat aggcttcgag ataaatatct
      961 ttccagatgc cgataggcgg aagctgcgga ccccaatccc agccgaaatg acagggagct
     1021 ttgcgcaggt gtggtccacc ggcgatggct tgcgaaacgc cgttcatggg gcgtgctgta
     1081 tttttctcgg ttacaaactt gacgggtgat ttgaacgtaa tggtcagttc gttgccttca
     1141 gctttgagca aggactttat atcaaaacga tattggcgga acatgttatt ggtttcgcca
     1201 agcgcttttc cattgagtgt gagtgacgca agcgtatcca acccatcaca aacaagccag
     1261 atatttttgc gctgaaagac atctgcctgc acattgaagg tggtgtgata ttcccagtcg
     1321 ctttcggcaa cccaggcaac tcgtttttca ttatcgccta caaatgggtc tgggatgcgt
     1381 gcaagcgcca tgagatcagt gtgaactccg cccggaacct gggctgggag ccattctata
     1441 gaacccaact ggcgaaactg ccataggctt gttagagatt gtttttgcat tggcattctt
     1501 cctgaatgtt ttcgattttt tattgctggt acttttcttt tatacggtgg agagatccca
     1561 ttcctcgtag atcttcggca cgtcgttaat gaggcgcttg gtataaggat gccgcggcgt
     1621 gagaatcact tcatccgcag agccgccttc tacaaatttg cctttttcca taatatagac
     1681 actatctgaa acatagtacg ccagaccaat atcatgcgtg atgaagatga tggtcatgcc
     1741 gatctctttg cggaggtgca tcaactcatc caaaatggtt gcacgagagc aggcatcgat
     1801 catggaggtt ggttcatcgg caaggagaat tttgggctgt aacaaaaaga tgcgggcgat
     1861 catgaggcgt tgcatttgtc cgccagaaag ttcgaaggga tatttattgg tcaactcttc
     1921 gaacttgagg ttcacgaagc tgcatgcctc agtcatcatt tctaatttct tttcacgtgg
     1981 caaatatttt ccgccgcgca tgttgatgca gtccagcaag accgagtcga tcttattgaa
     2041 gatgttatag gaagagaacg ggtcttggaa gatggcttga atgttcttcc aatattcctg
     2101 ttttctggac tgggtgctaa tgtcgcgctt gacgccttgg aagtacacat cgcccgatgt
     2161 ttggttcatc aatcccaata gcactttaga gagggtggtc ttgccgctgc ccgactcacc
     2221 cacaatggaa acaacttcac cttcatacaa gtcgaggtct acattatcca ccgccactgt
     2281 tttttggcgt ccaaacccga atgttttggt gatgcctttt gcgctcaggt agagtttctt
     2341 ttcacttggc atttttctgt tcctcctgct cacgagcata gattgcgcgc agttcagctt
     2401 ctgggatgat acagcgatac atacgtccat tgctcgcttc atgtaaaggc acacttgcga
     2461 cacggcattc cggtcgaaca aaacggcatc gttcggcaaa acgacaacct gaaggcggct
     2521 ttttgaggtt gggtggcacg ccaggaatgg cggtcag
//
LOCUS       DAOREF010000447         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29131_length_2557_cov_3.735012, whole genome shotgun sequence.
ACCESSION   DAOREF010000447 DAOREF010000000
VERSION     DAOREF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29131_length_2557_cov_3.735012"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKI33_09705"
     CDS             complement(<1..545)
                     /locus_tag="PKI33_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNM37329.1"
                     /translation="MSDLFVRKATGMVRSWSVFDAFIYAFFSINLVTLGFYSFSQMYY
                     FGGGMIPALIISALFIIFEVVVYASLIAVMPRSGGDYVWQTRVFGGGIGFILSITGWW
                     FTLWLWTPIYGDMLRQIVLTPLLGAFGFQQAAVWIAGSGTPLFVCSLLTLLFVALVIF
                     LGMKSYARVQKYSFYAGMLGL"
     gene            complement(591..1535)
                     /locus_tag="PKI33_09710"
     CDS             complement(591..1535)
                     /locus_tag="PKI33_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37330.1"
                     /translation="MTTTIFFATDIHGSDICWNKFLNAGKFYGADQLILGGDMTGKAV
                     VPFIHQGGPNYRVTLLEQVFEITNEDELTEMKKKVRSRGYYPYLTNPDEIKELEKDPE
                     KVSAIFSQEVLKVVQQWMEIAEKKLAGTGMKVYCCPGNDDMDEVDDVIRESRTVVLAE
                     GEVVDLPSGHEMIASGWSNRTPWNTHREEDEDQLAARYEAMISRLKNPQASIFNIHVP
                     PFKSGLDEAPELDKDLRPVLAGNSMIPVGSSALRKAIEKTQPLLSLHGHIHEGRGSTR
                     IGKTLVINPGSMYEQGTLLGAIIKLGKNKVENYVLTTG"
     gene            1799..>2557
                     /locus_tag="PKI33_09715"
     CDS             1799..>2557
                     /locus_tag="PKI33_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNM37331.1"
                     /translation="MKVFISSTYKDLIDYRAAAIRAVEGTNYQASKMEVFGARPDEPL
                     DACLKEVEESDFFLGIYAHRYGYVPSNLDISITEMEYVHAKKLGKPIYVFVIDEEKQP
                     WLPKFIEGEPGASKLRDFKQRVQTDHVCAFFTTADDLGMKVATALSHYVANHLPTSDS
                     QSPIYQSPKPTGNTLPHQEFFFGREKELESIKASLSPETRAWGALIDGPGGIGKTALA
                     IKAAHDAPAELFERKIFITAKVRELTAEGEAKLTD"
BASE COUNT          713 a          661 c          574 g          609 t
ORIGIN      
        1 aacagaccga gcatgcccgc atagaaggag tacttctgca cgcgcgcata agacttcatc
       61 cccaggaaga tgaccagggc tacgaaaagc agggtaagca atgagcacac aaacagcgga
      121 gtgcccgaac cagcaatcca cacggcggct tgttggaaac caaaggcacc cagcaatggc
      181 gtgagcacga tctggcgcag catgtcaccg tagatcggag tccacagcca gagcgtgaac
      241 caccagcccg tgatggaaag gatgaagcca atgccaccac cgaagacgcg ggtctgccat
      301 acgtaatcac caccagagcg cggcatgaca gcgatcaacg atgcatacac aacgacttca
      361 aaaatgatga atagcgcact gatgatcaac gcagggatca tcccgccccc aaagtaatac
      421 atctggctga agctgtaaaa accgagcgtc accagattga tggagaagaa cgcgtaaata
      481 aacgcgtcga agaccgacca ggaacgtacc atgccggtcg ccttacgaac aaacaggtca
      541 ctcataacaa ctctcctctt ttgatgttgt cttacacgcg aatgcgtggt ttaaccagtt
      601 gtcaaaacgt aattctcaac cttattcttc cccaatttga taattgcacc cagcaaagtt
      661 ccctgctcat acatggagcc ggggttgatc acgagggttt tcccaatgcg cgtggacccg
      721 cgcccttcgt gaatgtgccc gtgcaaacta agcaagggtt gggtcttttc aatggctttg
      781 cgcaatgccg aggacccaac cggaatcatc gagttgcccg ccaagactgg gcgcagatct
      841 ttatcgagct cgggagcttc atccaagcca gatttgaatg gcggtacgtg gatattaaaa
      901 atggatgctt gcggattctt caaacgagag atcatcgctt cataccgcgc cgcgagttga
      961 tcctcgtcct cttcacggtg cgtgttccaa ggcgttcggt tgctccaacc cgatgcgatc
     1021 atctcatgac cactcggcag atcgaccact tccccttcag caaggacaac cgtgcggctc
     1081 tcgcggatga catcatccac ttcatccata tcgtcattgc ccgggcagca atagaccttc
     1141 atgccggtgc cggctagttt tttctcggca atttccatcc actgttgaac gaccttcaac
     1201 acttcttgcg aaaaaatagc gctgaccttt tcagggtctt tctctaattc tttaatttca
     1261 tcgggattgg tgaggtaggg ataatagcca cgactgcgga ctttcttttt catttccgtc
     1321 agttcatctt cattggtgat ttcaaagact tgttcaagta aggtgacgcg ataattggga
     1381 ccgccctgat ggataaaagg cacaaccgct ttgcccgtca tatcaccgcc gaggatcaat
     1441 tgatcggcgc cgtaaaactt ccccgcattg agaaacttgt tccagcaaat atccgaaccg
     1501 tgaatatctg tcgcaaaaaa tatggttgta gtcacatgca aatccttcaa aatgaaatga
     1561 gcttgcagag gtggtgcggt ttaatgacgg ctattatact catcattaat gggatgtgaa
     1621 gataatatta acagccccaa tcaggtgaaa aagttacggt ttgtattttt tctcacaaca
     1681 cctgcgccga ttcggaaaat tccatgctat agttttacac aatggcttga cctgttctct
     1741 gcagacaatt atcatttcgt tctacacgtt tttactgggc taccacagga gatgaaacat
     1801 gaaagttttc atcagttcca cctataaaga tttgatcgac tatcgcgccg ctgccattcg
     1861 cgctgtcgaa gggacgaatt accaagcctc caaaatggaa gtctttggtg cgcgtcccga
     1921 tgaaccgctg gatgcttgct tgaaagaagt agaagaaagc gatttctttc ttggcatcta
     1981 tgctcatcgg tatggatatg ttccttcaaa tttggacatc tctatcaccg agatggaata
     2041 tgttcacgca aaaaaacttg gcaagccaat ttatgtattt gtcatagacg aggaaaaaca
     2101 accgtggctc ccaaaattta ttgaaggtga accaggtgca tcaaaattaa gagactttaa
     2161 acaaagagtg caaactgacc atgtttgtgc tttctttaca acagcagatg atttaggaat
     2221 gaaagttgct accgccctta gccattatgt agcaaatcac ttacctacgt ccgattccca
     2281 atccccaatt taccaatctc cgaaacctac tggcaacaca cttccgcacc aagaattctt
     2341 ttttggtcga gagaaagagc ttgaaagtat caaagcttct ctttcgcccg aaacaagagc
     2401 atggggagca ctcattgacg gacccggcgg catcggcaag accgcgctcg cgatcaaagc
     2461 cgcacacgat gcacccgcgg aactcttcga acgcaaaatc tttatcactg ccaaagtgcg
     2521 cgaactgacc gccgaaggtg aagccaaact gaccgac
//
LOCUS       DAOREF010000448         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29177_length_2555_cov_3.568400, whole genome shotgun sequence.
ACCESSION   DAOREF010000448 DAOREF010000000
VERSION     DAOREF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29177_length_2555_cov_3.568400"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKI33_09720"
     CDS             complement(<1..445)
                     /locus_tag="PKI33_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNM37332.1"
                     /translation="MATSIFSPYRICPIGAHIDHQGGSVLGRTIRIGTTLTYEPICLQ
                     EIHLTSERYGETTFRIGDLDLSHWARYAQAAARALKPTFGMQAHVHGSLIGAGLSSSA
                     SVGLAYLKALADVNKIELTPEQLIQLDFELEHDQLGLQNGLLDPMS"
     gene            complement(501..2183)
                     /locus_tag="PKI33_09725"
     CDS             complement(501..2183)
                     /locus_tag="PKI33_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNM37333.1"
                     /translation="MTTLPNLLQRSYQEIPDKASIILQHVGQADKPLTYRDLIRGANR
                     FALTYAREGIKPGEVVILILQHGEDLVYAFWGAILHGAIPSIMPFLTEKLAPEKYRAD
                     LASLISITKPTTIITYPEFEAEVRGALTDGDSVKHVILANTIEAESEPNFETLQGFKS
                     SPEDIVVLQHSSGTTGLQKGVALSHRAVLNQLNAYSQTLLIDPAQDVIVSWLPLYHDM
                     GFIACFLMPVLLRVPLVNMSPFDWVRAPYKLHQAVSQYKGTLTWLPNFAYNFCAHKIR
                     ERNLEGVDLSTWRAIINCSEPVHVESHDLFHEKFVAYGLKKSALQTCYAMAENVFAVT
                     QSPLDGIPAVDVIDRETFMTQRLATPPIAGQPSMKMTSSGNPLPNTKVRVVDADFNDL
                     PDRSVGEIAVQSDCMLTEYFNRPDATEAAIKNGWYLTGDYGYIADGELYVSGRKKDLI
                     IVGGKNVYPQDLESLASEVDGVHKGRVVAFGMYDEEEGTEEVVIIAEADSTVPSEQEA
                     IADAIRKYVTKSSAIALRHVKVVDEKWILKTSSGKTARSANKEKFLKGLGKI"
     gene            complement(2232..2477)
                     /locus_tag="PKI33_09730"
     CDS             complement(2232..2477)
                     /locus_tag="PKI33_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNM37334.1"
                     /translation="MTTEIITPIANFIAEKILKQPSKVISSAESLISSGLIDSFSLMD
                     LALFIEDTFGIRIEDTELNAETFDTLDQLAALISSRK"
BASE COUNT          592 a          611 c          699 g          653 t
ORIGIN      
        1 tgctcatcgg gtcgaggagt ccgttttgca gcccgagttg gtcatgctcc aattcgaaat
       61 caagttgaat gagttgttca ggtgtgagtt cgatcttgtt cacatccgca agcgctttga
      121 gataagccaa ccccaccgaa gcggacgagc tcaagcctgc tccgatcagt gagccatgga
      181 catgagcctg cattccaaat gttggtttca gtgcccgtgc cgccgcctgc gcataacgcg
      241 cccaatgcga aaggtcaagg tcgccgatgc gaaaggtggt ctctccgtag cgctcgctgg
      301 tgagatgaat ttcttgcaaa caaattggct catacgtgag cgtggtgcca atccgaatcg
      361 tacgccccaa caccgagccg ccttgatggt caatgtgtgc gccgatgggg cagatacgat
      421 acggtgagaa tatgctggtt gccatactgc ggcgagtcta acatgaaaaa aagagcatcc
      481 ttgtgagatg ctctttgcat ttatatcttt ccgagtcctt tcaaaaattt ttccttgttc
      541 gccgaacgcg cggtcttacc actggaggtt ttgaggatcc atttttcatc gaccactttg
      601 acgtgacgca gggcaatggc agaactcttc gttacatact tgcggatggc atccgctatc
      661 gcttcctgct ccgacgggac ggttgaatct gcttcggcga taataactac ctcttcggtg
      721 ccttcttctt catcatacat gccgaaggca acgactctac ctttgtggac gccgtctact
      781 tcgctggcaa gagattctaa atcttgcgga tacacgttct tgccgcccac aatgatgagg
      841 tctttcttgc gccctgaaac atacagttcg ccatcggcga tgtaaccgta gtcaccggtt
      901 aggtaccagc cgttcttgat cgcggcctct gttgcgtcgg ggcggttgaa gtattcagtg
      961 agcatgcagt cgctttgcac ggcgatctct ccaacggagc gatctggcag gtcgttaaag
     1021 tccgcatcca ccacacggac tttggtattt ggcagaggat taccagacga ggtcatcttc
     1081 atagatggct gaccagctat cggcggagtc gcgaggcgct gagtcatgaa agtctcgcgg
     1141 tcgatcacat ccacagcagg gattccatcc agcggacttt gagtcacggc gaagacattc
     1201 tctgccatcg catagcaagt ttgcagcgca gacttcttca agccatacgc cacaaatttt
     1261 tcgtgaaaga ggtcatggct ttcaacatgc accggttcag agcagttgat gatggctcgc
     1321 catgtggaaa ggtctacgcc ttcaagattg cgctcacgga tcttgtgtgc gcaaaagttg
     1381 taagcgaagt tcggcagcca cgtcagcgtg cctttgtatt gcgaaacggc ttgatgcagt
     1441 ttatacggcg cacgcaccca atcgaagggc gacatgttga cgagcggaac gcgcagcaac
     1501 acgggcatga ggaagcaagc gatgaagccc atatcgtgat aaagcggtag ccaagaaacg
     1561 ataacatctt gcgcggggtc gatgagcaag gtttggctgt aggcattgag ttggttgagc
     1621 acggcgcgat gcgaaagcgc cacgcctttt tgcaagccag ttgtgccgga ggaatgttgc
     1681 agcacaacaa tatcttcggg tgatgatttg aagccttgca gcgtttcaaa atttggttcg
     1741 ctctcggctt cgatggtatt tgcaagaatc acgtgcttta ctgaatcgcc atcggtcagt
     1801 gcgccgcgga cttcggcttc aaactcaggg taggtgatga tggtggtcgg cttggtgatg
     1861 gaaatcagcg aagcgagatc ggcgcggtat ttttcgggtg ctagtttctc ggtgagaaac
     1921 ggcataatgg agggaatcgc accgtgcagg atcgctcccc aaaacgcata gaccaaatcc
     1981 tcgccgtgtt gcaggatgag gatgacgact tcgccgggtt taattccttc gcgggcatag
     2041 gttaatgcaa aacgattcgc accacggatc aggtcacggt aagtgagagg cttatccgct
     2101 tgaccgacgt gttgcaggat gatgctggcc ttgtcgggga tttcttggta ggagcgttgg
     2161 aggaggttag gtaatgtggt catttaaatt acagtagggg cgtagcattg ctacgcccct
     2221 ttatgaatta attattttct tgaagagata agcgcggcca attgatcgag ggtatcaaac
     2281 gtctccgcat tcaattcagt atcttcaatg cggataccga aggtatcttc aatgaagaga
     2341 gcaagatcca tcaaactgaa agagtcgatc agtccgctgg agatgagaga ttcagcggag
     2401 gagatgacct tgctcggctg cttgagaatt ttctcggcaa tgaaatttgc aatgggggtg
     2461 atgatttcgg ttgtcatgaa taattccttt tcctgtgttt atttattgcg cattactttt
     2521 actgctcttg caacccccgt cttacagagt ccaat
//
LOCUS       DAOREF010000449         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29302_length_2549_cov_3.107859, whole genome shotgun sequence.
ACCESSION   DAOREF010000449 DAOREF010000000
VERSION     DAOREF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29302_length_2549_cov_3.107859"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PKI33_09735"
     CDS             complement(<1..768)
                     /locus_tag="PKI33_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNM37335.1"
                     /translation="MSETLKIQQLNDLKAQSRLGGGQARIDAQHKKGRLTARERLDLL
                     LDRGSFREVDPFVVHRTTDFGLDEQKIMSDSVVTGWGTIDGRLVYVYSQDFTVFGGSL
                     GEVHAEKICKIMDMAMKNGAPVIGLNDSGGARIQEGVVALGGYADIFLRNTMASGVIP
                     QISAIMGPCAGGAVYSPALTDFIFMTRNTSYMFVTGPDVVKAVTHEEVTQEELGGASV
                     HSEKSGVCHVAADSEADTLYLIRKLMSYLPQNNMEDSP"
     gene            complement(734..1147)
                     /locus_tag="PKI33_09740"
     CDS             complement(734..1147)
                     /locus_tag="PKI33_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37336.1"
                     /translation="MKRPFPVTLSVWLVLILASWNALRAWTALAWQDVLTEFSTQMPP
                     TLIVFASSFWLMIGIATLFSIWQEKPWSKNLLLGAAAGYAVWYWSERLIWQNPRPNIP
                     FTIVINFACLIVIYFASKSLSREAYERNTENPAIE"
     gene            complement(1151..>2549)
                     /locus_tag="PKI33_09745"
     CDS             complement(1151..>2549)
                     /locus_tag="PKI33_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37337.1"
                     /translation="FISMTSSVLLRTGFEAYNTTYAASAIEATIIAIVIKVVALYAFG
                     AYQNPRSTSIWKLLQKILFANLIGFVLSAGIYILLAQIGLGRNFPRTAFLLDFCISLI
                     LVSASRLAAYWFTKPGSIQAETPLEQFKSNWKVWLREGVVFYGIVGGLLLAYMLFNHF
                     AIGTSSPVSGQIKRWWGSIGNSAYERPAGTWQSYFGIGVDSYQAFQPFVETAWTTSDF
                     LQAYVLIKSNQEDERFLLTVFLYCSLWVVIIFLNKQRASQALTNLGVLPLLAISIIHI
                     LSYTATAYGGAKEWYWVSEMILITLMLSIFLDLVLRPLRRVQLLNLVLITVAVAYGVT
                     SANRFLQIIQYSMPRGYFPPDRPLMEVIGYLEENTFPGEVIGMTGGGNVGYFIKDRTV
                     INMDGLINSYEYFQVLKAGGAPTFLRDQGMTVIFANPSLLVLPPYYGQFAPYLEKYST
                     YGGKDLLYLLEEPKY"
BASE COUNT          742 a          559 c          584 g          664 t
ORIGIN      
        1 aggtgagtct tccatgttgt tttgcggcaa atagctcatc aacttgcgga tgagatacaa
       61 ggtatctgct tcgctatcgg ccgcaacatg acagacgccg gatttttctg agtgaacact
      121 tgcaccaccc aattcttcct gagtcacttc ttcatgagtt acagctttca ccacatcagg
      181 tccggtcacg aacatatagg atgtgttgcg tgtcatgaaa ataaagtctg tgagggcagg
      241 ggagtacacg gcaccgccag cgcagggtcc cataatggca gaaatctgtg gaattacgcc
      301 agatgccatg gtattacgca agaaaatatc ggcgtagcca cctaaagcca caacgccttc
      361 ttgaatacgt gcgccgccag agtcgttcaa accaataacg ggggcgccgt ttttcattgc
      421 catgtccatg atcttgcaga ttttttcggc atgcacttca ccaaggctgc caccaaagac
      481 ggtgaaatct tgtgaatata catacacgag ccgcccgtca atggttcccc agccagtgac
      541 gacagagtca ctcatgattt tttgttcgtc caacccgaag tctgttgtgc ggtggacgac
      601 gaacgggtcc acttcgcgga aggagccgcg atccaataaa aggtcaaggc gttcgcgtgc
      661 ggttaaacgt ccttttttat gttgagcgtc aatgcgggct tgtccaccgc caagacgaga
      721 ttgcgctttc aggtcattca attgctggat tttcagtgtt tcgctcatat gcctctctcg
      781 acaaagattt cgatgcaaaa tagataacga tcaaacatgc aaaatttata acaatcgtga
      841 acggaatgtt cgggcgtgga ttttgccaga tgagccgctc gctccagtac cagacggcat
      901 atcctgccgc tgccccaagg agcagatttt tactccaagg tttttcctgc caaatgctaa
      961 acaaggttgc tattccaatc ataagccaaa atgaacttgc aaagacaatc agagttggcg
     1021 gcatttgtgt agaaaattct gtcagaacat cctgccaagc gagggctgtc catgctcgta
     1081 gggcattcca agacgctaga attaacacca gccagacgct aagggttact ggaaaaggac
     1141 gcttcataga ttaatatttt ggctcttcaa gcagataaag caagtcttta ccgccgtagg
     1201 tgctgtattt ttcaagatat ggtgcaaatt gaccatagta gggcgggagc actaataagc
     1261 ttgggttggc gaaaatcact gtcattcctt gatctcttag gaatgttggt gcgcctcctg
     1321 ctttcaagac ttggaaatac tcgtagctat tgataagacc atccatattg atgacagtac
     1381 ggtctttgat gaaatacccg acgttaccgc cgcctgtcat gccaatcact tcaccgggga
     1441 aggtattttc ctctaagtat cctataactt ccatgagtgg acggtctggt ggaaaatatc
     1501 cgcgcggcat actgtattga ataatttgca agaatcggtt tgcactggtg accccatatg
     1561 cgactgcaac tgtaatcaat accaaattta aaagctgaac tcttcgtaag ggtcgtaaga
     1621 ctagatctaa gaagatgcta agcatcaagg taatcaagat catttcgctg acccaatacc
     1681 attcttttgc gccaccgtag gctgtagctg tataagaaag gatatgaata atgctaattg
     1741 ctaataatgg aagaacacct aagtttgtca gagcttgaga tgctctctgt ttgtttagga
     1801 aaataattac gacccataag gagcaataca agaagacagt taagagaaat ctttcatctt
     1861 cttgatttga ttttattaga acatatgctt gaagaaaatc agatgtggtc caagcggttt
     1921 cgacaaaagg ttgaaaggct tgataggagt caacgccaat gccaaaataa gattgccaag
     1981 ttcctgcagg gcgctcatag gcactatttc ctatgctacc ccaccaacgt ttgatttgtc
     2041 cgctgaccgg gctggaggtg ccaatggcaa aatgattgaa aagcatatat gctaataaaa
     2101 gtccacctac gatgccgtaa aaaacaactc cttcacgtaa ccagactttc cagtttgact
     2161 tgaattgttc aagcggagtt tcagcttgga tactgcctgg tttggtgaac caataggcgg
     2221 ctaggcgtga agcgctgaca agaatcaggc ttatgcagaa atctaaaaga aaagcagtgc
     2281 gggggaagtt tcgtcctaat ccgatttgag caagaaggat atagatgcct gcactaagaa
     2341 cgaaaccgat caagttggca aaaagaattt tttgtaatag cttccaaata gaagttgaac
     2401 gtggattttg atacgcgccg aaagcataga gcgcaacaac cttgataaca atggcaataa
     2461 tagtagcttc tattgcagat gcagcataag ttgtattata ggcttcaaaa cctgttcgca
     2521 aaagcacaga gcttgtcatt gaaataaaa
//
LOCUS       DAOREF010000450         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29380_length_2546_cov_2.563228, whole genome shotgun sequence.
ACCESSION   DAOREF010000450 DAOREF010000000
VERSION     DAOREF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29380_length_2546_cov_2.563228"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..513)
                     /locus_tag="PKI33_09750"
     CDS             complement(121..513)
                     /locus_tag="PKI33_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNM37338.1"
                     /translation="MPIVETSTPANTPTPIGPYNHIAKVGTFITIGGTAGVNPATGQL
                     AGDDIFSQTAQILDSFKVMLESVNSDLEHVVHINIFLKHMSDFEEMNRAYVSKMGDHR
                     PTRTVIGVNELPKPGVLLTMNLTAVTKE"
     gene            complement(515..1024)
                     /locus_tag="PKI33_09755"
     CDS             complement(515..1024)
                     /locus_tag="PKI33_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM37339.1"
                     /translation="MDLFETQRLLIRHLEVTDVDAMFGIFSLPEVTKWMGDGEPLSQE
                     LCAKWIEVSQKNYATKGFGASAVIEKASGEFIGCSGIVYDSERQEPEIIYAFHPSAWR
                     KGYASELVPAMLNYGLTECGLTYILATIAEANKASAHVVEKAGMKFVTEEPNPDGHIT
                     LVYRIEKGK"
     gene            1149..1808
                     /locus_tag="PKI33_09760"
     CDS             1149..1808
                     /locus_tag="PKI33_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37340.1"
                     /translation="MLNKIFSDPKRIVILILSAIIALFICVVVLIGGGWSILHRVDLT
                     PTTEPVSFGYCGAEFTSLCVVSFGRDALGNTVINLYVPIRRYPIFYLKVVRQSGEQRF
                     ECESNRDVKESVYCTGSPLNLGEGFNIQVVSEKDNIILAEGEFSLIAFRVTTPMVGGV
                     DSINTPGVLNTESTPSIETKKTPTPSQPISESGTVTPTTSDSIVETKTLTPQSSYPSY
                     P"
     gene            1809..>2546
                     /locus_tag="PKI33_09765"
     CDS             1809..>2546
                     /locus_tag="PKI33_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37341.1"
                     /translation="MSVWFDGLSALSDHIWTVLIILVLCFWGVFVIRFFLQRELEKSL
                     TEIELFTLSAVGWIFPLLLLSILTFFVSLFTNLVVGGVFFVLVFFSSTFLIVREKINV
                     LLALKFLLFLIMFIVLRFAFLKNLFLPSYFDSAEHYRLISLINAAYRFGVISSELIDS
                     FYHLGFHFISAFFSVFLQVHVIDFMFVFGQVVLAFLTPALFFVIKRETGSSSSAVLTC
                     VLSTFGFHMPVYLMNWGKYPALSGLIAM"
BASE COUNT          617 a          499 c          571 g          859 t
ORIGIN      
        1 aaaagatatt gcgccacttg cgggtcactg aaatgttgaa acgtgaagtc gagatcatct
       61 gcttgaaacg ggcgcagaga cagtcgttcg gtttggatgg gtggaaaatc ttgcactgac
      121 ttattccttt gttaccgctg tcaaattcat cgtaagcagc acgccgggtt tggggagttc
      181 gttcacgccg atgaccgtcc gcgtggggcg atggtcgccc attttgctga cataagcgcg
      241 gttcatctct tcaaagtcgc tcatgtgttt gaggaagatg ttgatatgaa cgacatgctc
      301 caaatccgaa ttgactgact ccagcatgac cttgaaagaa tcgagaattt gcgcggtttg
      361 tgaaaagatg tcatctccgg cgagttgacc ggtggcggga ttcacgcctg ccgtcccgcc
      421 gatggtaatg aaggtgccca ctttggcgat gtggttgtac ggtccgatcg gcgtcggggt
      481 gttggctggg gtggaggttt caacaatggg catattattt tcctttttca atacgataga
      541 caagggtgat gtgtccatca gggttaggct cttcggtgac aaacttcatg cctgcttttt
      601 ctacaacatg agcagatgct ttattcgctt ctgcgattgt ggcgaggatg taggtcaacc
      661 cacattcggt cagcccatag ttcaacatgg cagggaccag ttcactggcg tagcctttgc
      721 gccatgcgct gggatgaaag gcataaatga tctccggctc ctgccgttct gagtcgtata
      781 caatgccgct gcaaccaatg aactcacctg aagctttttc gatcacagcc gaagcaccga
      841 agcctttggt ggcatagttt ttctgtgaga cttcgatcca cttcgcgcat agttcttggc
      901 ttagtggctc tccatctccc atccattttg ttacttcggg caggctgaag ataccaaaca
      961 tagcatccac atctgtcact tcaaggtgac gaatcaataa acgttgcgtt tcaaataagt
     1021 ccatttttgc tcctaagctt tttaagttga aaacattctc tagaacggtt ctgaaattaa
     1081 cgttgcttcc gtggtctgca ttatatacat attaatacac agaattttgc aatggtagaa
     1141 tgggaataat gctgaataaa attttttcag acccaaaacg tattgtgata ttaatattaa
     1201 gcgcaatcat agctctgttt atttgtgttg tcgtgttaat tggtggaggg tggtcaatat
     1261 tgcatcgagt ggaccttact ccgactaccg agcctgtatc ttttggctat tgcggtgcag
     1321 aattcacaag tttgtgcgtg gtgtcatttg gacgtgatgc attaggcaat acggttatta
     1381 atttatatgt accaataagg agatatccaa tcttttattt gaaagtggtt aggcaatctg
     1441 gagaacaaag gtttgagtgc gaatcgaacc gtgatgtaaa agaaagtgtg tattgcacgg
     1501 gctctccatt gaatttaggc gaaggcttca atattcaagt tgtttctgaa aaagataata
     1561 ttatccttgc cgaaggagaa ttttctttga tcgcattccg cgttacaact ccaatggtag
     1621 gaggagtaga ttcgataaat acccctggcg tactcaatac agaatcaaca cctagtatag
     1681 agacgaaaaa aactccgaca ccatctcagc ctatttctga atctggcact gttactccga
     1741 caacatctga cagtattgtt gaaacgaaaa cattgacacc gcaatcttct tatccaagtt
     1801 atccgtaaat gtctgtttgg tttgatggac tttcagcact gtctgaccat atttggacag
     1861 tgcttattat tctcgtttta tgtttttggg gggtgttcgt cattagattc tttttgcagc
     1921 gagaattaga aaagtctctg acagaaatag aactctttac cttaagtgct gtaggttgga
     1981 ttttccctct tctattactt tctattctta ctttttttgt ctcacttttt actaacctag
     2041 ttgttggggg agtattcttt gttttggtat ttttttcttc tacctttctc atcgttcgag
     2101 aaaaaatcaa tgtgctactt gcacttaaat ttcttttatt cttgattatg tttattgttt
     2161 tgcgttttgc atttcttaag aatttatttt taccatcgta ttttgactct gcagaacatt
     2221 atcgtctcat aagcctgatt aatgctgcat atagatttgg tgttatttct agcgagttaa
     2281 tagatagttt ttatcatttg ggctttcatt ttatttcagc ctttttttct gtttttctac
     2341 aggttcatgt tattgatttt atgttcgtgt ttggacaggt tgtgctagct tttttgacac
     2401 cagccctttt ttttgtgatt aagagggaga ccggatcaag ttcctccgct gttttaactt
     2461 gtgtgttgag taccttcggt tttcatatgc cagtatactt gatgaattgg ggaaagtacc
     2521 ccgctttgtc ggggttaata gcaatg
//
LOCUS       DAOREF010000451         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29577_length_2535_cov_4.388306, whole genome shotgun sequence.
ACCESSION   DAOREF010000451 DAOREF010000000
VERSION     DAOREF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29577_length_2535_cov_4.388306"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PKI33_09770"
     CDS             <1..290
                     /locus_tag="PKI33_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37342.1"
                     /translation="LSTQQRYRRLQNLGERIVAELRERGLNAEAEEVASVLRNNAQTS
                     AGKEAPAPQKRAVLPTHCPSCGAAVRPDEVEWLDDVTAECDYCGSPVRAES"
     gene            292..891
                     /locus_tag="PKI33_09775"
     CDS             292..891
                     /locus_tag="PKI33_09775"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNM37343.1"
                     /translation="MTEITEEYIWNKLNTTDRSRVETGGYAEIPIHPDVQLKCAAVLV
                     PLVFFQNEWHVLYTRRTDRVESHKGQVSFPGGACDEGETTPEETALREADEEIGLNPK
                     DVKVIGKLSRMVTISKFKVTPVVGIIPFPYAFKTSGAEVARVFTMPLLWLANRNNYWE
                     FSMPGSGRTLLAYHPYDGELLWGATARMTVNFLSLLDLL"
     gene            1113..1928
                     /locus_tag="PKI33_09780"
     CDS             1113..1928
                     /locus_tag="PKI33_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM37344.1"
                     /translation="MKVQNKVMVVTGGGNGMGRELVLNLLAKGARVAAIDINETALKE
                     TAQLAKEYKENIATFVLNITDKDAVDALPAKVIAKFGKVDGIINNAGIIQPFVRLVDL
                     DYSAIQRVLDVNLYGTIYMVKAFLPHLKERPEAHITNVSSMGGFLPVPGQTVYGASKA
                     AVKLLTEGLYAELLNTNVKVTLVYPGAIGTNIAANSGITVPNMPEADSKEASNFKQLA
                     PDKAAEIIVNAIENNRYSVLVGSDAKFMDFIYRLNPQNAAKFIYNQMKALLPS"
     gene            complement(1994..>2535)
                     /locus_tag="PKI33_09785"
     CDS             complement(1994..>2535)
                     /locus_tag="PKI33_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNM37345.1"
                     /translation="LEPISIVLDAHTAGLALSKVSASSLVTIDVEGKEYPALVRERQR
                     DFIKGVLTHVDFLAVDMNEKIRTSVGLAFVGVSGAVKDYNGILVHNLERLEVECLPGD
                     LPERINVDISLLKQIGDIFRVKDLAISEKVRILADEEEPIAVVTITKDEASAQSAAAG
                     TGEATSPELSVERGKKDEE"
BASE COUNT          689 a          610 c          631 g          605 t
ORIGIN      
        1 tactaagcac acaacaacgt tatcgccgtt tgcagaacct tggcgaacgc attgtagcgg
       61 aattacgtga acgcggcttg aatgccgaag ctgaagaagt agcatccgtt ctcaggaaca
      121 atgcccaaac gtcggcgggg aaagaagcgc cagcaccgca aaagcgagcg gttttgccaa
      181 cccattgtcc atcgtgtggt gcggcagtcc gcccggatga agtcgaatgg ctggatgatg
      241 tgaccgcaga atgtgattac tgcggcagcc cggtgcgcgc tgaatcttag tatgaccgag
      301 attacagaag aatacatttg gaataaactt aatacgaccg accgttcgcg cgtggaaacg
      361 ggcgggtatg ccgagattcc gattcatcca gatgtgcagt tgaaatgcgc ggcggttctg
      421 gttccgttgg tattcttcca aaatgaatgg catgtgctat acactcgccg cacagaccgg
      481 gttgaatcgc acaaagggca agtctctttt cccggcggtg cttgcgatga aggcgaaacc
      541 acgcccgaag agaccgctct gcgcgaagcc gatgaagaga tcgggttgaa tcctaaagat
      601 gtaaaagtga tcggcaaact cagccgcatg gtgacgatca gtaaattcaa agtgacccct
      661 gtggttggga ttattccttt tccttatgcg tttaagacct ctggtgcaga agtggcacgg
      721 gtctttacca tgcctttgct ttggctcgcc aaccgcaata actattggga attttccatg
      781 cccggctctg ggcggacttt gctcgcctat cacccatatg acggtgaact tctctggggc
      841 gccacagcac gcatgacggt caacttcttg tctctcttag atttgctgta gtttctccag
      901 atatataact tgaagttaga tttaggacaa tttccaccct ctatattgtg aaaaatatca
      961 caataatcct ctttatttgt gattaatttc actaataaaa tctctccctc agacctacaa
     1021 tacagacgat cagaacgatc aaacaacaat ctcggtgccc ctccccttat ggaggtgaaa
     1081 gcaacccaag gattgataaa ggagagatca acatgaaagt acaaaataaa gttatggtag
     1141 taacgggcgg cggaaacggt atgggacgtg agttggtgtt gaacctttta gcaaaaggcg
     1201 cacgtgtggc tgccatcgat atcaacgaaa ctgctttgaa agaaaccgct caattggcga
     1261 aagagtacaa agaaaatata gcgacattcg tcttgaacat tacagataaa gacgctgtcg
     1321 atgcgttgcc tgccaaagtg attgccaaat tcggaaaagt agatggcatc atcaacaacg
     1381 cgggcattat tcagcctttc gtgcgactcg ttgacttaga ctattccgcc attcaacgtg
     1441 tattggatgt aaacctgtac ggcaccatct acatggtcaa agcttttctg ccgcatttga
     1501 aggaacgccc tgaagctcac atcaccaacg tttccagcat gggcgggttc ttgccggtcc
     1561 ctgggcaaac ggtttatggc gcgagcaagg ctgcagttaa gttactaacc gaaggtttat
     1621 acgcggaatt actgaacacc aacgtaaaag tgaccttggt gtacccgggc gccattggta
     1681 caaacatcgc tgcgaattcg ggcatcacag taccgaacat gccagaagca gacagtaaag
     1741 aagccagtaa ttttaagcag ttagcgccag acaaagcggc agagatcatc gtgaatgcca
     1801 tcgagaataa tcgctacagc gttctcgtag gctcagatgc aaagttcatg gatttcatct
     1861 acagactgaa tccgcaaaat gcagccaagt ttatctataa ccaaatgaaa gccttgctac
     1921 cttcgtaata cattggtaaa aaaagtccgc catcttgcga tggcggactt ttttattgct
     1981 agtacagaag aaattattct tcgtctttct tgccacgttc cacgctgagc tcgggagacg
     2041 tcgcttcgcc ggtgccggca gcagcagact gggcagaagc ctcatccttg gtgatggtca
     2101 ccacggcaat gggttcttct tcatccgcca agatgcgaac tttttcggag atcgcgaggt
     2161 ctttcacgcg gaagatatcg ccgatctgct tgagcagaga gatatctaca ttgatgcgtt
     2221 caggaagatc acctggtaaa cattccactt cgaggcgttc caagttgtgg acgaggatac
     2281 cgttgtaatc cttcacggcg ccagaaacac ccacgaaagc gagtcccacg ctggtgcgga
     2341 tcttttcgtt catgtccacg gcgaggaagt ctacatgggt gagaacacct ttgataaagt
     2401 cacgctggcg ttcgcggacg agagcagggt attccttgcc ttccacatca atcgtcacga
     2461 ggctggaagc agaaaccttc gacaaagcca agccggcggt gtgtgcatcc aaaacaatag
     2521 aaatcggttc gagat
//
LOCUS       DAOREF010000452         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29610_length_2533_cov_5.212672, whole genome shotgun sequence.
ACCESSION   DAOREF010000452 DAOREF010000000
VERSION     DAOREF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29610_length_2533_cov_5.212672"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKI33_09790"
     CDS             <1..221
                     /locus_tag="PKI33_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNM37346.1"
                     /translation="EGKPALSAEEAEEFKRPLLEKYEREGSPYHSSARLWDDGVIDPA
                     DTRQVLGLALSVVLNAPKEDGGFGVFRM"
     gene            224..703
                     /locus_tag="PKI33_09795"
     CDS             224..703
                     /locus_tag="PKI33_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HNM37347.1"
                     /translation="MTHKESSPHHFLAKLAGHWQGTSKLWFEPDKLANEAPIVGTIQI
                     ILDGRYALFLYQSSIEGEAQHGMFTFGYNTQLDRYETSWVDSFHTNTSIMFCTGNEIE
                     NGFSVLGSYPDPTGGPDWGWRTDVQMNGDELIVTAYNILPEGLEAKATEIILKKVNA"
     gene            700..1686
                     /locus_tag="PKI33_09800"
     CDS             700..1686
                     /locus_tag="PKI33_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM37348.1"
                     /translation="MKILIIGGTRFIGRHLVTSARARGHEVTLFNRGKSNPGLFRRIQ
                     NIRGDREKDLDQLSGQWDAVIDTCGYLPRVVRLSAEALKEKVSRYVFISSVSVYANFS
                     KVGIKESDAVGTLADESVEEITGETYGPLKALCEKAVQDVYGARSLIIRPGLIVGPHD
                     PTDRFTYWPLRIAKGGDVLTPDRPDAMAQFIDARDLADFIIRLIDQNVSGTFNAVGYP
                     VTLNTIFETCKRISKSNANFKWAPIDFLEKNNVAPWSDMPIWVPEVGEDAGVSQVDVS
                     KAVAAGLTFSPLTDTVKAIYDWEFERPAEYEMKAGLKPEREAELLKLLNHAH"
     gene            1676..>2533
                     /locus_tag="PKI33_09805"
     CDS             1676..>2533
                     /locus_tag="PKI33_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNM37349.1"
                     /translation="MLTKILIANRGEIAIRIMRACRELGIKTVAVYSEADKNAQHVQF
                     ADEAILLGGAAPKESYLNVDALLRAARDSQADAIHPGYGFLSENASFAAAVESANLTF
                     IGPSADSIRAMGDKAESKILMKKAGVPTVPGYEGLESYDDFKNAAKDIGYPVLVKAAA
                     GGGGKGMRVVNAESELQEAIELARREALNAFGDERLLIEKYLAEAHHIEIQVFGDKHG
                     NLVHLFERECSVQRRHQKIIEESPSPLLTPELRAKMGQAAVNAAKAVNYYNAGTVEFI
                     FDPKAQQYYF"
BASE COUNT          654 a          664 c          622 g          593 t
ORIGIN      
        1 gagaaggcaa accagccctg agcgcggaag aagcggaaga gttcaaacgt ccgctgttgg
       61 aaaagtacga acgcgaaggc agtccctatc attcgtccgc ccgtctctgg gatgatggtg
      121 taatcgaccc tgccgataca agacaggttt taggcttagc attatccgtt gtgctgaatg
      181 cgccaaaaga agatggcggc ttcggcgtat ttaggatgta accatgacac ataaagaatc
      241 ctctcctcat cattttcttg caaaacttgc cggtcactgg cagggtacca gtaaactctg
      301 gtttgaacct gataaactgg ctaacgaagc tcccatcgtt ggaaccattc aaatcatcct
      361 cgatggacgt tacgcgctct tcctatatca atcttccatt gaaggtgaag cccagcatgg
      421 catgttcacc ttcggctaca acacccaact cgaccgctac gagaccagtt gggtcgattc
      481 cttccatacc aatacatcca tcatgttctg taccggtaat gaaatcgaga atggattctc
      541 tgtgcttggc agttatcccg accccactgg cggacccgat tgggggtggc gcaccgatgt
      601 acagatgaat ggtgacgaat tgatcgtcac tgcctataat attttgccag aagggcttga
      661 agccaaagcc actgaaatca ttcttaaaaa ggtgaatgca tgaagatcct cattatcggc
      721 gggactcgtt ttatcggaag gcatctcgtc acgtctgccc gtgcccgcgg acatgaagtc
      781 acgctcttca atcgcggaaa aagcaacccg ggtctttttc gaagaatcca aaacattcgg
      841 ggagatcgag aaaaagattt ggatcagtta tcggggcagt gggatgcggt cattgatacc
      901 tgcggctacc tgccgcgcgt cgtccgcctc tctgctgaag cgctgaaaga aaaggtcagt
      961 cgctatgtat ttatttccag cgtttcggtg tatgcgaact ttagcaaggt tggcatcaaa
     1021 gaaagcgatg ccgttggcac ccttgccgat gaaagcgtgg aagagatcac tggcgaaacc
     1081 tatggtcctc taaaagcttt gtgcgaaaaa gcagtgcaag atgtatatgg tgcccgctca
     1141 ctgattattc gccctgggct cattgtgggt ccgcatgacc ccactgaccg tttcacctat
     1201 tggcctttac gcatcgccaa aggtggagat gtgctcactc cagaccgccc cgatgcaatg
     1261 gctcaattta ttgatgcgcg cgacctcgct gatttcatca tccggctcat tgaccaaaat
     1321 gtttcgggca cattcaatgc tgttgggtat cccgtcacgc tcaatactat ttttgaaacc
     1381 tgtaaacgga ttagtaagag caacgccaat ttcaaatggg cgcctataga ttttcttgaa
     1441 aagaacaacg ttgccccatg gagcgatatg cccatctggg ttccagaggt cggcgaagat
     1501 gccggggttt cgcaggtgga tgtttcgaaa gcggtagcag ccgggcttac cttctcgccg
     1561 ctcacagata ccgtcaaagc catttacgac tgggagtttg aacgccccgc agagtatgaa
     1621 atgaaagcag gcttaaagcc tgagcgtgaa gccgagttac ttaaattgct caaccatgct
     1681 cactaaaatc cttatcgcca atcgcggcga gatcgccatt cgtatcatgc gtgcctgtcg
     1741 tgagcttggc atcaaaacag ttgccgtcta ttccgaagcg gataagaacg cacaacacgt
     1801 gcaattcgcc gatgaagcca tcttgcttgg gggtgcagca ccgaaagaat cttatttgaa
     1861 tgtggatgcc ttgctacgag ccgcacgcga ctctcaagcc gatgccattc acccgggcta
     1921 tggcttcctc tcagagaacg cctctttcgc cgccgcagtg gaatcagcga acctaacctt
     1981 tatcggtccc tcagccgact cgatccgtgc catgggcgat aaagctgaat caaagatatt
     2041 gatgaagaaa gccggtgtgc ccactgttcc aggttacgaa ggcttagaaa gttacgacga
     2101 cttcaaaaac gccgccaaag atattggcta ccctgtcctc gtcaaagccg cagctggcgg
     2161 aggcgggaaa ggcatgcgcg tggtcaatgc tgaaagcgag ttgcaagaag ccattgaact
     2221 cgcacgccgc gaagccctca atgctttcgg tgacgaacgc ctgctcattg aaaaatatct
     2281 cgctgaagca catcatattg aaattcaagt ctttggcgac aaacacggca acctcgtgca
     2341 tctcttcgaa cgtgaatgct cggtgcagag acgtcaccaa aagatcatcg aagaatcccc
     2401 ttccccattg ctcacccccg aactgcgcgc caaaatgggg caagctgcag tcaatgctgc
     2461 taaagccgtc aactactata acgcaggcac tgtcgaattc atcttcgacc cgaaagctca
     2521 gcaatattat ttc
//
LOCUS       DAOREF010000453         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29848_length_2523_cov_5.152755, whole genome shotgun sequence.
ACCESSION   DAOREF010000453 DAOREF010000000
VERSION     DAOREF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29848_length_2523_cov_5.152755"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PKI33_09810"
     CDS             complement(<1..584)
                     /locus_tag="PKI33_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNM37350.1"
                     /translation="MTIEFEAPKPIVQMQAMLKTVAENMMRSTSREMDENEHAIPWDY
                     VEFMHTAMRSMGGGGGLTITEPKPKEGEEKRPPIGYQKLATQIEMLAWGDVGFYLITP
                     GGGLGAAAVAAAGSPEQKAKFLERFNSDKPTYAAMCMTEPGAGSDTSAIRARAVLDEK
                     TNEWVINGEKIFVTGGDKSFNEYEKLGKGFIVVWA"
     gene            complement(674..1762)
                     /locus_tag="PKI33_09815"
     CDS             complement(674..1762)
                     /locus_tag="PKI33_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNM37351.1"
                     /translation="MYSFEPNEEQQMLIDAVGKFASNDLRPAAHDAEENHAMPQKLVN
                     KGWELGLLQASTPEAYGGFGDRSTVTNVLAVEEMAFGDLAGALAVMTPSLFATPILLG
                     GSEEQKQEYLPKIIEGAWAPYTGALIEYSFNFDPNELKTTANAEGGAYVLNGEKAFVP
                     FAKDAEALIVYANLNGVTQGFIVKKEAAGLSISDEREKLMGLNALPMYRVKLENVTAE
                     RLGGASGHDFGLILDAMRVASAAAALGVAKSSLDYAKNYAKEREAFGVKIAQKQAVAF
                     MLAEMAVELESMRLMTWEAAWKIDTNKEDTCVSAYLAFTGACDMAMMVTDRGVQIYGG
                     HGYIRENPVELLMRNGRGFPMLLGLAIV"
     gene            complement(1804..2364)
                     /locus_tag="PKI33_09820"
     CDS             complement(1804..2364)
                     /locus_tag="PKI33_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNM37352.1"
                     /translation="MTREDVLDAAAQVFRQKGFHGASMSDIAAAVNVQKASLYHHVSS
                     KQEILLALLDRALEMLTEHISTISAQNISPDQKLRIMIRGYLTGLAENADLTSVLLFE
                     HRSLDKKSHARHVPLRDKFEKLWHDVLEEGVEKNIFSLSDTGLATRAIMGTLNWTLTW
                     YRPEGGKSIEQIADEYSEFILKGMLK"
BASE COUNT          595 a          685 c          609 g          634 t
ORIGIN      
        1 gcccacacca caatgaaccc cttgccaagt ttttcgtatt cattgaaaga cttatcgccg
       61 ccagtgacaa agatcttctc gccattgata acccactcgt tggtcttctc gtccaaaaca
      121 gcacgcgcac gaatcgcgga tgtatccgaa ccagcaccgg gttcggtcat acacatcgcg
      181 gcataagtcg gcttatcgct gttaaagcgc tcaaggaatt ttgctttctg ttcgggggag
      241 cctgccgcag ctaccgccgc cgcgccaagt ccaccaccag gggtgatgag ataaaaaccc
      301 acatcgcccc aagccagcat ttcaatttgg gtggcaagtt tctgataccc aatgggcgga
      361 cgtttctctt caccttcttt gggtttcggt tctgtaatgg tcaagccacc gccgccgccc
      421 atggagcgca tggcagtgtg cataaattca acgtaatccc acggaatggc atgttcgttc
      481 tcatccattt cacgcgaggt gctgcgcatc atattctctg cgacagtctt caacatcgcc
      541 tgcatttgga cgatgggctt cggggcttca aattcgatgg tcatgattct ctcctttagg
      601 tcaaaggttg cagggtgcag gttgaacgtt ttaatacaac ctgtaacttg cgacttgtaa
      661 cctgtaactg gttctacaca attgcaagtc ctaaaagcat tgggaaaccg cgcccgttac
      721 gcatgagcaa ttccacgggg ttctcgcgga tatagccgtg tccaccatag atttgcacgc
      781 cacggtcggt caccatcatt gccatgtcac aggcaccggt gaaggcaagg tatgcagata
      841 cacaagtatc ttccttgttg gtatcaattt tccaggcggc ttcccatgtc atcaagcgca
      901 tggattcgag ttcgactgcc atttccgcca gcatgaatgc aacggcttgc ttctgcgcga
      961 tcttcacacc aaaggcttca cgctccttgg cataattctt tgcataatcc agcgaggact
     1021 tggctacgcc caaagcggca gcagcggagg caactctcat cgcgtcgagg atcaagccaa
     1081 agtcgtgtcc tgaggcgccg ccgagtcgtt cggcggtcac attctcgagt ttgacgcggt
     1141 acatgggcag cgcattaagt cccatcagtt tttcgcgttc atcagagatg gataaacccg
     1201 ccgcctcttt cttgacgatg aagccttgag tgaccccatt gagatttgcg tacacgatga
     1261 gcgcttcggc gtctttggca aacggcacga aagctttctc gccgttcaaa acataagcac
     1321 caccttcggc gttggcagtg gtcttgagtt cgttcgggtc gaagttgaaa ctgtattcga
     1381 tgagtgcgcc ggtgtaagga gcccacgcgc cttcgatgat cttcggaaga tattcctgct
     1441 tttgttcttc actgccgccc agcaaaatgg gagtagcaaa cagcgatgga gtcatcacgg
     1501 caagagcgcc agccagatcg ccaaacgcca tctcttccac agcgaggacg ttggtcaccg
     1561 tgctgcgatc accaaagcca ccatacgcct caggcgtgga cgcctgcaat agtcccaact
     1621 cccagccctt gttgacgagt ttctgcggca tcgcatgatt ttcttcggcg tcatgcgcgg
     1681 caggacgcaa gtcgttggaa gcgaacttcc ccaccgcatc gatcaacatt tgttgttctt
     1741 cattaggttc aaacgagtac atatgtcttc tccttttaca tttggcgcaa agcaactatt
     1801 caactatttc aacatgccct tcaaaataaa ctctgaatac tcatcggcga tctgctcgat
     1861 ggattttcca ccttcggggc gataccaggt taaagtccag ttcaaagtgc ccatgatggc
     1921 gcgtgtggca agtccggtgt cgctcaatga aaatatgttt ttctccacac cttcttctag
     1981 aacatcgtgc catagttttt caaacttatc tcgcaaggga acatggcggg catgactttt
     2041 cttgtccagc gaacgatgct caaacaacaa gaccgaagtc aggtcggcgt tttcggcgag
     2101 ccccgtcagg tagccgcgga tcattattct caatttttga tcgggggaaa tgttttgggc
     2161 ggagatggtg gagatatgct cggtaagcat ttcaagagcg cggtctaaca gcgccaggag
     2221 aatctcctgt ttggatgaga catggtggta caggctggcc ttttgcacat tcaccgccgc
     2281 cgcaatatca gacatggacg cgccatggaa ccccttctgg cgaaagacct gtgctgcagc
     2341 atcaagaaca tcttctcgag tcatcgagtc tccttcccta ccgaacggta ggtaggcgta
     2401 atatactacc gtcataaggg agagtcaaga gatttcatcc tcctctacac aactttactg
     2461 ggatataaaa tagacctctt gcataagtat gccataatag gcagatgaac tatgaaacca
     2521 tcg
//
LOCUS       DAOREF010000454         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29849_length_2523_cov_4.919368, whole genome shotgun sequence.
ACCESSION   DAOREF010000454 DAOREF010000000
VERSION     DAOREF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29849_length_2523_cov_4.919368"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="surE"
                     /locus_tag="PKI33_09825"
     CDS             complement(<1..531)
                     /gene="surE"
                     /locus_tag="PKI33_09825"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNM37353.1"
                     /translation="MTKRILVTNDDGVLAPGLLALAKEMRNLGDVTILAPDRNWSGGG
                     HVKTLDRALRVREFRLEDGSQAFASDGAPSDCVSLALLGYFKEKFDLVVSGINAGANL
                     GHDVTYSGTVTAAMEAVITGVPGVAVSLEIPEGTLAPIDYSASTYAARVAVENVFAHG
                     LPEDTLLNVNIPFLKKE"
     gene            complement(589..1296)
                     /locus_tag="PKI33_09830"
     CDS             complement(589..1296)
                     /locus_tag="PKI33_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNM37354.1"
                     /translation="MGLADLHLHTIYSYDGTATVPAVLRRARELGLNVIAITDHDEIA
                     GALEAVKLASHYGVEVIPGSEITTAEGDLLALFIHEKIQPGLSLIETLLRVKEQGGVA
                     IAAHPMAGGMGMKSLTPASILKALKHPVAKKVLIGIETYNGTAIDRVSNHYTRIFAQG
                     LKIAHTGNSDAHIIDTIGFGATEFPGTTAADLLNALRERTTRVRKMNEWSAFRVMSSW
                     GFRYIESAFVRLKLVRA"
     gene            1767..2267
                     /gene="sixA"
                     /locus_tag="PKI33_09835"
     CDS             1767..2267
                     /gene="sixA"
                     /locus_tag="PKI33_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNM37355.1"
                     /translation="MNLYLIRHAIAEEESPAVEDRLRALTEKGSKKMRNIAKGLKTLG
                     LEFDYIISSPYVRAQQTAEILGDVFKLRKRIVISESIAPMGDPSQLLAEINEKYTVNS
                     LAIVGHEPYLSNLISLLTAGGAPLEMTFKKGGVCYLTTDDLHHTRKATLQWLLTPGVL
                     VEIGEG"
     gene            2318..>2523
                     /locus_tag="PKI33_09840"
     CDS             2318..>2523
                     /locus_tag="PKI33_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37356.1"
                     /translation="MTEIDLSHPSLYINREASLLEFQRRVLEEARDEENPLLERVKFL
                     AILGSNMDEFFMVRVSGIRKQVEA"
BASE COUNT          618 a          575 c          702 g          628 t
ORIGIN      
        1 ttcttttttc aagaaaggaa tattgacgtt gagcagcgtg tcttcgggca agccgtgcgc
       61 gaagacgttt tcaactgcca cgcgagcggc ataggtggag gcagagtaat cgatgggggc
      121 aagggtgcct tctggaattt ctagcgaaac cgctacaccc ggcacaccgg tgatgacggc
      181 ttccatcgcg gcggtgacgg tgccagagta ggtcacatcg tgcccgaggt tggctccggc
      241 attgatgccc gatacgacga ggtcgaactt ttctttgaaa taaccgagca atgccaggga
      301 gacgcaatcg ctgggggcgc cgtcgctggc gaaggcttgc gagccatctt cgaggcggaa
      361 ctcgcgcaca cgcagggcac ggtctagggt tttgacatga ccgccgcccg accagtttcg
      421 gtctggggcg aggatggtca catcaccgag gtttcgcatt tctttggcga gggcgagcaa
      481 gccgggagca agtacgccgt cgtcgttggt gacaaggatt cgtttggtca tggttgttgt
      541 ctctttatat agtttcccct attgtaatga tgaaagataa gagaagattt atgcacgcac
      601 caactttagg cggacaaaag cgctttcaat gtagcgaaag ccccaactac tcatcacgcg
      661 aaaggcgctc cattcgttca ttttgcggac gcgggttgtg cgttcgcgca gggcgttgag
      721 caagtctgcg gcggtggtgc cggggaattc tgtcgcgccg aagcctatgg tgtcgatgat
      781 gtgtgcgtca ctgttgccgg tgtgggctat tttcaaccct tgggcaaaga tgcgagtgta
      841 gtgattgctt acccggtcaa tggcggtgcc gttatacgtc tcaataccaa tcaacacttt
      901 tttagctaca ggatgcttaa gtgccttcaa aatggaggcg ggagtcaggc ttttcatgcc
      961 catgccgcct gccatggggt gagcggcaat tgccactccg ccttgttctt taacacgcaa
     1021 caaggtttca atgagggata gccctggttg aattttttcg tggatgaaga gcgcgagcaa
     1081 atcgccttca gcggtggtga tctcacttcc ggggatcact tccacgccgt agtgtgaggc
     1141 gagtttgacg gcttccaacg cgccagcaat ttcgtcatga tccgtaatgg cgatgacatt
     1201 taatccgagt tctcgtgcgc ggcgtaacac agcagggacg gtggcggtgc catcgtagct
     1261 gtagattgta tgcaagtgta ggtctgcaag tcccattgta ttctccgttt ctatatctct
     1321 ttagaccaaa atgcgttcgg aaagtttcaa gaatatagtt gtagtgtagt gtggagttgt
     1381 taatgggagt tgaacttgtt gtgtggtttt tgttaacgat tccacccgcc tgtcgctgtc
     1441 aggcgggtgg atgaaggaag gaggagaaga aaaatgaaaa agaactacgc gcggctttgt
     1501 ctttgcaaaa actcgcgacg tttgcgagaa gcgtctatgg tgaagacggt caacaaagag
     1561 agaaataaga aaagagcgaa gtacgtttcc atgcctgtat cttacaacag tcatttgatt
     1621 ttggacttaa gaaccagtta acaacgggta aacgattatg caaggcggag gcgaaaacca
     1681 cctgtacaga tttatttaca tattcttaat gaggaaaatt ttgcagcctg tataattcga
     1741 gtactttatt ttcaggagag cgccctatga atttatatct gatccgccat gccattgccg
     1801 aagaagagtc gcctgcggta gaagaccgtc tgcgcgcgtt gaccgagaaa ggctccaaaa
     1861 agatgcgcaa catcgccaag gggcttaaga cccttgggct ggagtttgac tacatcatta
     1921 gcagcccata cgtccgcgct caacaaacgg cagagatttt gggcgatgtc tttaagctta
     1981 ggaagcgcat tgtgattagc gaaagcattg ctcctatggg agacccgagc cagttattgg
     2041 cggagatcaa tgaaaaatat acggtcaata gccttgcgat cgttgggcat gagccgtatc
     2101 tgtcgaatct tattagccta ctaacagctg gcggcgctcc tcttgaaatg acctttaaga
     2161 aaggcggggt ttgctacctt acgacagatg acttacatca cacccgcaaa gcaaccctgc
     2221 aatggctatt aacacccggt gtgctggttg aaattgggga aggataatag aatacaggga
     2281 ttcttttgca cgcccatcct tccttgagga aaacctaatg accgagatcg acctgagcca
     2341 tccttcttta tatatcaacc gtgaagcaag cctattagag ttccaacgcc gcgtcctaga
     2401 agaagcgcgc gatgaagaaa atccgctgct tgaacgcgtg aagtttctgg caatcctcgg
     2461 ctcgaatatg gacgagttct ttatggtgcg tgtctctggc atccgcaaac aagtggaagc
     2521 caa
//
LOCUS       DAOREF010000455         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29879_length_2522_cov_4.126064, whole genome shotgun sequence.
ACCESSION   DAOREF010000455 DAOREF010000000
VERSION     DAOREF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29879_length_2522_cov_4.126064"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKI33_09845"
     CDS             complement(<1..255)
                     /locus_tag="PKI33_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37357.1"
                     /translation="MKWVIAMLVAVTAGYMLFDGVHALRTGDYITPSEGEYAGQLGPW
                     AGLVASVGIDPHSTLMKTIFVAYGFSTLVGVAGFLTDQVWG"
     gene            complement(252..743)
                     /locus_tag="PKI33_09850"
     CDS             complement(252..743)
                     /locus_tag="PKI33_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37358.1"
                     /translation="MSTNHAHYLIEEYAFHNWLVHHILEGIDHEESLLQLPFESNSTN
                     WILGHIVTNRSHVLEVLGVTHLWQEEVRGLYHTGTHNIGPGDKAMRLETLLKYMDDST
                     RMIRRALEPVSEGWLAESFTNYRGEKTREAHLSGFHWHEGFHIGQLEMLKDFVLSTRK
                     VLA"
     gene            complement(762..1220)
                     /locus_tag="PKI33_09855"
     CDS             complement(762..1220)
                     /locus_tag="PKI33_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM37359.1"
                     /translation="MSEDFRKNLLNFMTETFESPMGIYLDKNTALFQTLETVSAAEAS
                     IPVGGKCATLAAQVAHVTYYLDVLERYVVHNDTSPADWGEVWRTVSGVTTQEWETSKA
                     KLRSAYQRMVELFKTNPVWNETTMGGAMAIVVHSAYHLGEIRQALCTLKK"
     gene            complement(1220..1789)
                     /locus_tag="PKI33_09860"
     CDS             complement(1220..1789)
                     /locus_tag="PKI33_09860"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNM37360.1"
                     /translation="MSSLDKAVQTQLENIQKKTGKSLAELAAIAQKSGLTKHGELRDM
                     FKEKLGLGHGDANALVHAVFKSDGTRAAEGKSSDAVLDEIYVGAKASQRPIHEALMKQ
                     INKFGEFEVVPKKGYVSLRRKKQFAMIGPKTNTRFEVGINAKEMKKNPRLVEQPKGSM
                     CNYIVALTDASEVDAELIGWIKLAYEGAD"
     gene            2024..>2522
                     /locus_tag="PKI33_09865"
     CDS             2024..>2522
                     /locus_tag="PKI33_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37361.1"
                     /translation="MNRLVLLAGLIITLVGCTTKSTPPLPTPIPATATQSVPTPTPVP
                     PTPTLVEIPVQVNAEVANCRFGPGTVYQTMNEVRGGRLLNAVGRDAASTWWQVEDPIN
                     PGGYCWVAAEVTTLQGTAEQLPIVDAPFVSVTKVDLRVEPNRIVVNCNDFPQTVFFEA
                     NITANG"
BASE COUNT          621 a          710 c          544 g          647 t
ORIGIN      
        1 cccccaaact tggtctgtga gaaaaccagc aaccccaacc agcgtagaaa aaccataagc
       61 aacaaaaatg gttttcatca atgtggaatg tgggtcaatg cctacagaag cgacgagccc
      121 agcccaaggt ccgagttgac ccgcgtattc gccttcagat ggcgtgatgt aatcgcccgt
      181 ccgcaaggca tggacgccgt cgaagagcat gtatcctgcc gtgacggcaa ccagcatggc
      241 aatcacccac ttcatgccaa cacctttcgg gtagaaagga caaaatcttt cagcatttcc
      301 aactgaccaa tatggaaacc ttcgtgccag tggaatccgc taaggtgcgc ttcacgggtc
      361 ttttcgccgc gatagttggt aaagctttct gccagccacc cctcgctgac aggctccagc
      421 gccctgcgaa tcatgcgcgt actatcatcc atgtacttca aaagagtttc caagcgcatg
      481 gctttatcgc cgggaccaat attatgagtg ccggtgtgat acagaccacg cacttcttcc
      541 tgccagaggt gtgtgactcc caatacttcc agcacatgac tacgattggt aacgatatga
      601 ccgagaatcc aatttgttga gttggattca aagggcagtt gtaacaaact ttcttcgtga
      661 tcgatccctt caaggatgtg atgaaccaac cagttatgaa aggcgtattc ttcaattagg
      721 taatgtgcat gattggtcga catgtatatg tttcctcttt tttatttttt caacgtgcaa
      781 agcgcctgac gtatctctcc taggtgatag gcactgtgaa caacaattgc catggctccg
      841 cccatcgtgg tttcgttcca aactgggttt gtcttgaata actccaccat gcgttgatag
      901 gcacttcgca atttggcttt actcgtttcc cattcttgcg tcgtgacgcc gctcaccgtg
      961 cgccaaacct cgccccaatc ggcaggcgat gtatcgttgt gaactacata acgctccagc
     1021 acatcaagat aataggtcac atgcgcaacc tgtgccgcga gcgtggcgca cttcccgcca
     1081 acgggaatcg aagcctccgc tgcagagacc gtttcgagcg tctgaaacag agccgtgttc
     1141 ttatcaaggt aaattcccat ggggctttca aaggtctcgg tcataaagtt caaaagattt
     1201 tttctaaaat cttccgacat taatcagctc cttcatatgc aagtttgatc cagccaatca
     1261 gttcagcatc cacttcgctg gcatccgtca gggcaacgat gtaattgcac atactgcctt
     1321 tgggttgttc taccaaacgc ggatttttct tcatctcttt ggcattaata cccacttcaa
     1381 aacgagtatt ggttttagga ccgatcatcg caaattgctt cttgcgccgc aaacttacat
     1441 atcccttctt cggcacaacc tcaaactctc cgaacttgtt gatctgtttc atcaacgcct
     1501 catggatggg gcgctgcgag gctttggcac cgacgtaaat ttcatctaaa acagcatcag
     1561 agcttttccc ttcggcagcg cgtgtaccat cagacttgaa aaccgcatgc accagtgcat
     1621 ttgcgtcgcc atgcccaagc cccagtttct ccttgaacat atcgcgcagt tcgccatgtt
     1681 tggtcagccc gctcttttga gcaatggctg ccaactctgc cagcgattta ccggtcttct
     1741 tttggatgtt ttccagttgt gtttgaacgg ctttatcaag actactcata ctctcatctc
     1801 cttttgaata aaacaaccga gttacacaat ggtacaatta tagaacaaat tttctaattt
     1861 tgcaattgct tatccccaca aagtaccaat tccattctat gagcgcatct gaacttcagc
     1921 ttccctgtta cacttctata gaacatgcag gtgattcgac ctcgaattgc cttgcctctc
     1981 caacgccgat ggagagttga aacgaatgac aaaaggagga aaaatgaacc gcctcgtcct
     2041 gcttgcagga ctcattatca ccttggtggg atgcacaacg aaaagtacgc cgcctctccc
     2101 cacgccgatt ccggctactg ccactcaatc tgttcctacc cccacacctg ttccacccac
     2161 ccccaccctt gtagagattc ctgtacaggt gaacgcagaa gttgccaact gccgttttgg
     2221 tcccggcacg gtctaccaaa ccatgaatga agtccgcggt gggagactct taaacgctgt
     2281 cgggcgcgac gcggcatcta cctggtggca ggtcgaagat cccatcaacc caggcggata
     2341 ttgctgggtt gcagccgaag tcaccacctt gcaaggaacc gcggagcaac tacctattgt
     2401 agatgccccc ttcgtcagcg tcaccaaagt ggatttgcgc gtggagccga atcgtatcgt
     2461 cgtcaactgc aacgactttc cgcagaccgt cttcttcgaa gccaacatca ccgccaacgg
     2521 ac
//
LOCUS       DAOREF010000456         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_29992_length_2517_cov_5.203899, whole genome shotgun sequence.
ACCESSION   DAOREF010000456 DAOREF010000000
VERSION     DAOREF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29992_length_2517_cov_5.203899"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1066
                     /locus_tag="PKI33_09870"
     CDS             218..1066
                     /locus_tag="PKI33_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37362.1"
                     /translation="MINWQELTIDMFVEQLRTSYRRTYGDIQSEYGNIVSWVGRLALE
                     NISNSDALYHDIDHTIMVSLAGLAIIEGKHLREGGVSARDWMRYMIAVLCHDIGYVKG
                     VCKNDTDELFATGEGTERIKISKDGTDVALTPYHVNRSKLFVMERFGSGLLKDMEEHL
                     NAELITTYIEMTRFPSPKGEFYQDTQGLGGLVRAADFVGQLGDPDYFRKTPALFYEFQ
                     ELGANEKLGYQSPNDMRKNYAKFYWEQVNPYLKDALVYLRLTEDGKQWIANMHSHVFD
                     SEHTYK"
     gene            complement(1098..1334)
                     /locus_tag="PKI33_09875"
     CDS             complement(1098..1334)
                     /locus_tag="PKI33_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37363.1"
                     /translation="MNELSIAQKIIRFFVSEEAFDAMKAESEAWIVKCLNCGHERSIW
                     SMGGIRYKATGNSRLYRICPNCSQRSWQTISKRK"
     gene            complement(1331..2278)
                     /locus_tag="PKI33_09880"
     CDS             complement(1331..2278)
                     /locus_tag="PKI33_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNM37364.1"
                     /translation="MNEVSNETRFLHAVEMGLGAWQWGDRVVWGYQPGESDKGAREGF
                     DVSLNMGVRFVDTAEVYGSGRSERLLGQFLKETDQPVLVATKFFPWPWRFSKSFIPRA
                     LKASLERMGLDSVDLYQIHWPSPLLSPESLMEGLAICVKEGLARTVGVSNFWESRMLR
                     AYSALAQQGIPLASNQLPFSLLKRDAEKNGTLARCKELGIRFIAYSPLSQGLLTGKYS
                     IDNPPPGVRGSNYAEALKKLPPILKVLQEVAQNHGKTVSQVALNWTICKGALPIPGAK
                     NARQAEDNAGGAGWRLTDDEVALLDEVTDNFRDYGGTVK"
BASE COUNT          652 a          608 c          608 g          649 t
ORIGIN      
        1 gcgcagccac ccagcccaaa gagggctggc aagccacagt cagacgaaag agccgaggca
       61 aggatattga gtagaagaaa gattgagagt ccgatcagga accagttgag aaagatccgg
      121 cgtttcgtca taacaattcg atttctgtct gttttctttt tgtatgtttc tggtaaactt
      181 ttggcactat tcaattttac ccttatagga tgtctttatg atcaactggc aggaattgac
      241 catcgatatg ttcgtggaac aattgcgtac ttcgtaccgc cgaacctatg gtgatattca
      301 atcggagtat ggcaatattg tttcttgggt ggggcggctt gcgttggaaa acatttcgaa
      361 ctcagatgcg ctgtatcacg atattgacca taccattatg gtttctcttg ccgggcttgc
      421 cattattgaa gggaagcatt tgcgcgaagg cggagtgagt gcgcgcgatt ggatgcgtta
      481 tatgatcgcg gtgctttgcc atgatattgg ctatgtaaag ggtgtgtgca agaacgacac
      541 cgatgaattg tttgcaacgg gggaggggac ggaaagaatt aagatttcaa aagatggcac
      601 cgatgtggcg ttgacccctt accacgtgaa ccgcagtaag ttgttcgtga tggaacgctt
      661 tgggtctggg ctacttaaag atatggaaga acatctcaac gctgaattga tcacaacgta
      721 tattgagatg acacgcttcc cttcaccgaa gggtgagttt tatcaagaca cccaaggttt
      781 gggcgggttg gttcgtgcgg cagattttgt cggccagctc ggagacccgg attatttccg
      841 caagactccg gcgttgttct atgaatttca agaattaggc gcgaatgaaa agcttggcta
      901 tcaatctccg aacgacatgc gcaagaatta tgccaagttc tactgggagc aggtcaaccc
      961 gtatttgaaa gatgccttgg tgtacctgcg cctcacggaa gatggcaagc agtggatcgc
     1021 caacatgcac tcccacgtgt tcgactcgga acatacatat aaataaaaaa gcgccgcagg
     1081 ttctctgcgg cgctttttta ttttcgcttt gaaatcgtct gccaactgcg ttgactgcaa
     1141 ttcgggcaaa tgcgatataa acgcgagttg cctgttgctt tgtaccgaat gccgcccatg
     1201 ctccagatac tgcgttcgtg tccgcaattt aagcatttca cgatccaagc ctcagactct
     1261 gctttcatgg catcgaacgc ctcttcagag acaaagaagc ggatgatttt ttgtgctatc
     1321 gatagttcgt tcacttaaca gttccgccat agtctctaaa attatcggtg acttcatcta
     1381 acaaagccac ttcatcgtct gttaagcgcc aacctgcacc accggcatta tcttccgctt
     1441 gccgcgcatt ttttgcgccg ggaatcggta aagcgccttt gcaaatggtc caattcaatg
     1501 ccacctgaga gacggtcttg ccatggtttt gggcaacttc ttgcaaaacc ttgagaatag
     1561 gcggtaattt tttgagtgcc tctgcgtaat tcgaaccgcg tacaccggga ggcggattat
     1621 caatagagta cttccccgtt agcaaacctt gagaaagcgg agaataagca atgaagcgga
     1681 ttcccaattc tttacagcgt gccaacgtgc cattcttttc agcatcgcgt ttgagaaggc
     1741 tgaagggtaa ttgattcgag gcgagtggaa ttccctgttg cgccaaggca gaataggcac
     1801 gtaacatgcg cgattcccaa aagttggaaa cgccaactgt acgcgccaag ccttccttaa
     1861 cacagatcgc caacccttcc atcaaggact ctgggctgag aagcggagac ggccagtgga
     1921 tctgatacaa atcaacagag tctagtccca tgcgttccaa gctggctttg agcgcccgcg
     1981 gaatgaaact ttttgagaag cgccagggcc aagggaagaa tttcgtagct accaatacag
     2041 gttggtctgt ttcctttaaa aactgaccca ataaacgttc agaccgtcca ctcccataca
     2101 cttcggctgt gtccacgaag cgcacaccca tgttcaacga aacatcaaac ccttcacgag
     2161 cccctttgtc tgattccccc ggttggtatc cccaaacgac acgatctccc cattgccacg
     2221 cacccaaacc catctccacc gcatgcaaaa atctggtttc attgctgact tcgttcataa
     2281 gggtctcctt gaatggcgca gattttatct taaaaaaccg ctttgccaaa agattcaggt
     2341 tacaattcgg ctcgctaaaa tctatataag aactcaagga acgacatctc ctcacttagg
     2401 aggtaagtgg atatcgctcc cggagttctg ggaggttccc gtgacacaac aatcaaataa
     2461 gcgggatgtg gaatccgctt ccagcctgaa cgagaggctg ggtctgtcct tttccaa
//
LOCUS       DAOREF010000457         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30022_length_2516_cov_5.832995, whole genome shotgun sequence.
ACCESSION   DAOREF010000457 DAOREF010000000
VERSION     DAOREF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30022_length_2516_cov_5.832995"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..473)
                     /locus_tag="PKI33_09885"
     CDS             complement(168..473)
                     /locus_tag="PKI33_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37365.1"
                     /translation="MSQAPMMEVSSDDKLWAALAYAFSPLIPIILMFMEDKKNRPFIK
                     AHNMQALILGIITVVTSGFCIGILVWIYALYCAYQAYQGLEVKIPVLSDFVKNQGWA"
     gene            635..1552
                     /gene="fmt"
                     /locus_tag="PKI33_09890"
     CDS             635..1552
                     /gene="fmt"
                     /locus_tag="PKI33_09890"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNM37366.1"
                     /translation="MSIRVVFMGSPDFALPSLRALVDRYNVVGVVTQPDRASGRGREL
                     KAPPVKLLAQDLNIPVMQPQKLREPEAMQQLQAWNPDLIVVAAFGQILRKDVLVLPKY
                     GCVNVHASLLPRWRGAAPINASVLAGDEETGVTIMKMDVGLDTGPMLSMEKIRIKPDD
                     TAGALFEALSTLGAKLLIETLPGYINGTVLPQPQPEEGATYAPMLKKEDGRLDFNQPA
                     VELERRIRAMNPWPGAWFEWNGNILKVSRGAVSEAKGKEVGSRLIVEGRPAVQCADGV
                     LILEEVQPPSKKVMPGKAFLSGTRNWESS"
     gene            1604..2353
                     /locus_tag="PKI33_09895"
     CDS             1604..2353
                     /locus_tag="PKI33_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNM37367.1"
                     /translation="MFNWKVFLAEFIGTFALVFIGAGAGVVQAGLVGVALAHGLTVMV
                     FAYSFGHISGTHVNPAVTFGLALQGVVKWGEAVVYWVAQCLGAISAAGLLHLVVSQLG
                     GTINAGATVGALTDKFPVYAMLIEAVLTFFLLNTILHTAVAGKGGQFAGLAIGLTLTA
                     AILVGGPMTGASLNPARTLGTAVYTSPSLANVNTYVTYLFGPLIGATAAVLIFNVFKT
                     ETPVAEEEISVPVEEPAKPATKRKTTAKAKK"
BASE COUNT          637 a          534 c          668 g          677 t
ORIGIN      
        1 tcttctgcag ggctgagcgg ggcaagtggt tgttgaggta tttgttcgtt catcatattc
       61 tcctaatagt atacaaactc tttgaattaa tacgtgtata ggagaggcaa agtagcaaaa
      121 taaaagaccc gcgtgttaca cgcgggtctt ttgtagagcg agactgatta tgcccagccc
      181 tgatttttga cgaaatcgct aagaacaggg atcttaactt ccaagccttg gtacgcctgg
      241 tatgcgcaat acagagcgta gatccagacc aaaataccaa tgcagaaacc ggaagtgaca
      301 acggtgataa tgccaaggat caaagcctgc atattgtgag ctttgatgaa tggacgattc
      361 ttcttatctt ccatgaacat taaaatgatc gggataaggg gggagaaggc gtaagcaaga
      421 gctgcccaca gcttatcatc actcgatact tccatcatag gtgcttgaga catggtttat
      481 tcctccgaat aagttgtttg aattttaagt attttacaac caatctacac aacgtgcaac
      541 caaactaatt cacccaatgg tactgatacg ttacgaagag cccgttttgc tgaaaattct
      601 ccttaattat tgagaccagc ggtaaaatct gcatatgtct attcgtgttg ttttcatggg
      661 ttcgcccgat tttgctcttc cgagcctccg cgctcttgtt gatcgctata atgttgttgg
      721 ggtggttacc cagccagatc gtgcctctgg gcgcggacgt gaactgaagg cgccgcctgt
      781 taagctttta gcgcaagacc tcaatatccc cgtcatgcag cctcaaaaat tacgtgagcc
      841 tgaagcgatg caacagttgc aagcttggaa tcctgatttg attgtggttg cagcttttgg
      901 tcaaatctta agaaaagatg tcttagtctt gcccaaatat ggttgtgtca atgtgcatgc
      961 ttcactgttg ccacgttggc gtggagcggc accgatcaat gcatctgtgc tggctgggga
     1021 tgaagagacg ggcgttacca ttatgaaaat ggatgtgggg ttggatacag gtcccatgct
     1081 ttcgatggaa aaaattcgca tcaagccaga cgatacagcg ggcgcactct tcgaggctct
     1141 atctacgctg ggagcgaaac ttcttattga gaccctgcct gggtacataa atggcactgt
     1201 cttgccacaa cctcaacccg aagaaggtgc aacctatgcc ccgatgctca aaaaagaaga
     1261 tggacgatta gactttaatc aaccagctgt ggaattggag cgaagaattc gtgcgatgaa
     1321 cccatggccc ggagcatggt ttgagtggaa cgggaacata ctcaaagtaa gtagaggtgc
     1381 tgtcagcgaa gcaaaaggca aagaagtggg gagcaggctt atcgtcgaag gaagacccgc
     1441 agtacagtgt gcggatggag tcttaatttt ggaggaagtc caaccgccta gcaagaaggt
     1501 tatgccgggg aaggcgtttt tatccggcac acggaattgg gaatcgtcgt agcaaattga
     1561 caggtatggg gttataactt ccaaattaat ctttggagga tgtatgttca attggaaagt
     1621 atttcttgct gaatttatcg gcacgtttgc gttggtgttt atcggtgcag gggcgggcgt
     1681 ggtgcaggct gggctggtgg gcgtggcgct tgcacacggc ttgacagtta tggtcttcgc
     1741 ttattcattt ggtcatattt cgggtactca tgtgaacccg gcggtaacct ttgggttggc
     1801 attgcagggt gttgtgaaat ggggcgaggc ggtagtgtat tgggtggcgc aatgccttgg
     1861 ggcgatttcg gcggctggat tgcttcattt ggttgtctct caactcggcg ggacgatcaa
     1921 tgctggagct acggtaggcg cattgaccga taaattcccg gtgtatgcca tgttgatcga
     1981 agccgtgttg acattcttcc tgctcaacac aattttgcat acggctgtag caggcaaggg
     2041 cgggcagttt gctgggttgg caatcggctt gacattaacc gctgccattc ttgtaggtgg
     2101 accgatgacc ggtgcatctc ttaacccggc acgcacttta ggaaccgctg tttatacaag
     2161 cccaagcttg gcgaacgtga atacctatgt cacatacttg ttcggtccat tgattggtgc
     2221 cacggcggct gttttgatat tcaacgtatt taagactgag acacctgtag ccgaagaaga
     2281 gatttctgtt ccggttgaag aaccagcaaa accggcgaca aagcgcaaga caactgcaaa
     2341 agcaaagaaa taaattttgt gcttctttca agccttcgcc ttcaaagcga aggctttttg
     2401 ttttacaatt aatccatcgg aggcactctc atgactcaat acgttggcgc gattgatcaa
     2461 ggcacgacca gtactcgttt tattattttc gaccacaacg gcaatgtggt tgcagt
//
LOCUS       DAOREF010000458         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30024_length_2516_cov_4.953677, whole genome shotgun sequence.
ACCESSION   DAOREF010000458 DAOREF010000000
VERSION     DAOREF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30024_length_2516_cov_4.953677"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..914)
                     /locus_tag="PKI33_09900"
     CDS             complement(36..914)
                     /locus_tag="PKI33_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNM37368.1"
                     /translation="MEITLALGGGGAKGNAHIGVLRRLEKEGYKIRSVAGTSFGGMVA
                     ALYAIGYSPREIQAIFESVDQESLYGRASQDGPSLLGMAGVRQLLERVIGEKTFKDTR
                     IPCAVTAVDINTGGEVVLNEGSIKNAVLATIALPGIFPVQKINDWELVDGGVLNPVPV
                     SVARLLSPDLPVVAVVLNDPIDKPVRGYNLPLPSIVPRTIAERLARISLAQSLDIFLR
                     SVDVSSRYVSHLRLQVDKPEIIVRPDVHHIELLDQVVVEDVAALGEQALEQVLPDLKR
                     KTTWASRLGRRVFGVN"
     gene            complement(1017..2033)
                     /locus_tag="PKI33_09905"
     CDS             complement(1017..2033)
                     /locus_tag="PKI33_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNM37369.1"
                     /translation="MRKRISFLFALIILLVLSITPKQTVEAKPFANITSPSQLIDAVN
                     SLRVANGLSPLPVHQALMQSAQSQADYMAATGNVTHSRPSGTYTQQLLALGFPLAGDL
                     SAGGFRSENILSTYGPLVWNGVPSAWQDAQHMNTMLSGNFTHIGAGVSQSGDMYYYTV
                     DTAAATSSGQQQDSAATVLTSVPGGSSDAGVSQFMVPVTLNTPRPDGNVYHKVQYGQS
                     LWSIAIAYGTTIKNIQALNNLGEASTVYQGQELLVVTGATQAAAPTLDATATTQATAT
                     LAPTSTNVPTSTPELALLTPTEEAPVEANTKPASSRVLIVVLIVAAFVGAGMAVWLIR
                     DPGN"
     gene            complement(2042..>2516)
                     /locus_tag="PKI33_09910"
     CDS             complement(2042..>2516)
                     /locus_tag="PKI33_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNM37370.1"
                     /translation="RPEVQHLLKPLVVSWGYEAEAPGDSQFIDYNEWWGTRDVSAFLT
                     VPKAIQFQQDQNWAEVRAACHRLAVETWQRIHELTGETPLHSDPEPWFAQMAVATLPA
                     DSDLVALKSRLYDEHRIEIPLIAWNEKKLIRLSVQGYNSRRDMDKLLSALNTLLR"
BASE COUNT          658 a          644 c          601 g          613 t
ORIGIN      
        1 gtcacgcata tacttgcgca aatcacgagt catttttaat tcactccaaa tacacgtcga
       61 cccaaacggg aagcccatgt ggttttgcgc ttcaggtctg gcaatacttg ctctaatgcc
      121 tgctccccta aagcagccac atcttccaca acaacctgat ctaacaattc aatatggtgc
      181 acatccgggc ggacaatgat ctcgggctta tccacttgca agcgcaaatg cgaaacatag
      241 cggctggaaa catctacaga gcgcaagaag atatccaagg actgggctaa cgaaatcctt
      301 gcgagccgtt cagcaatcgt acgtggaaca atgctgggca gcggcaagtt gtatccgcgc
      361 acgggtttat caatggggtc gttcaacaca acggcaacca ccggcaggtc tggcgataag
      421 agccgagcca cagacaccgg caccggattc aagacgccgc catccactag ttcccaatcg
      481 ttgattttct gcaccgggaa gatacccggc aaagcgattg tggcgagtac agcatttttg
      541 atactgcctt cattaagtac cacctcaccg cccgtgttga tatccacggc agtgacggca
      601 caaggaatgc gagtgtcttt gaaggttttc tcaccgatca cacgttctaa cagttgacga
      661 accccagcca ttcccaatag agacggacca tcttgcgagg cacgcccata aaggctttcc
      721 tggtctactg attcaaagat tgcttgaatt tctcgcggag agtagccaat agcatataaa
      781 gcagcgacca ttccaccaaa actagtccct gcgacagagc gaattttata tccttctttt
      841 tcaagacgac gtaacacgcc aatgtgagca ttgcccttgg cgcccccacc gcctaatgcc
      901 aacgtaattt ccattttctt cgatgccttt caatattcaa ttaacggaca aatcaaacgc
      961 gagggattat acccttacaa aaaaacaaca tagtttcaac acattttctg agaaacttaa
     1021 ttccccgggt cgcgaatcag ccaaactgcc attcccgccc ctacaaatgc cgccacaatc
     1081 aaaacgacaa tcaacactct tgaagaggct ggctttgtat ttgcttcaac gggtgcttct
     1141 tctgtagggg taagcagtgc cagttctggg gttgaggtag gaacgttggt tgaagttgga
     1201 gccaaggttg ccgtggcttg tgtcgtagct gtggcatcca aagttggagc cgctgcttga
     1261 gtggcgcccg taaccacgag caactcttgc ccttgatata cggttgaggc ttcgccaagg
     1321 ttgtttagcg cttgaatgtt cttgatggtt gtgccatagg caatggcaat actccacaac
     1381 gattgcccat actgcacctt atgatacaca tttccgtcgg gacgaggcgt attcaatgtg
     1441 accggcacca tgaactgact gacgcccgca tcacttgacc cgcccggcac gctggtgaga
     1501 accgttgcag cactatcttg ttgttgaccc gagctggttg cagcggcggt atccacagtg
     1561 taataataca tatcgcctga ttgtgaaacg ccagcgccaa tgtgtgtaaa gttgcctgaa
     1621 agcatagtgt tcatatgttg agcatcttgc caagcgctcg gaacaccatt ccatactaaa
     1681 ggtccgtagg tacttaagat attttcggag cgaaagccgc ccgcacttag atcgccggct
     1741 aacggaaagc caagcgcaag caattgctgg gtgtaggtgc cactggggcg agagtgtgtt
     1801 acattgcctg tagctgccat gtaatctgct tgcgattgag cagattgcat caatgcctga
     1861 tggacaggca agggtgaaag cccatttgcg acccgcaaac tattgaccgc atcgatcaat
     1921 tgggatgggg atgtaatatt tgcaaatggt tttgcttcaa cagtttgttt tggggtaatg
     1981 ctcaatacga gcagaataat caaggcgaat aaaaacgaaa tccgttttct catatatgct
     2041 ttcatcttaa caacgtattc aatgcagata ataatttgtc catatctcga cgagaattat
     2101 atccctgcac agacaaacgg attaatttct tctcattcca cgcgatgagc ggaatttcaa
     2161 tacgatgctc atcatacaag cgcgatttga gggcaacgag gtcagagtcc gctggcaggg
     2221 tggcaactgc catttgggca aaccaaggtt cggggtcaga atgaagcggg gtttctcctg
     2281 tcagttcatg gatgcgctgc catgtctcca ctgccaggcg atgacatgcg gctcttacct
     2341 ccgcccagtt ttggtcctgt tgaaattgaa tcgccttcgg gacagtaagg aaggcagaaa
     2401 catcacgagt gccccaccat tcgttgtaat caataaattg cgaatcgccg ggcgcctcgg
     2461 cttcatagcc ccacgaaacc accaagggtt ttagcaggtg ttgaacttca gggcgg
//
LOCUS       DAOREF010000459         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30047_length_2515_cov_4.652033, whole genome shotgun sequence.
ACCESSION   DAOREF010000459 DAOREF010000000
VERSION     DAOREF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30047_length_2515_cov_4.652033"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..965)
                     /locus_tag="PKI33_09915"
     CDS             complement(243..965)
                     /locus_tag="PKI33_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNM37371.1"
                     /translation="MKARRDISFAIVVLILSILACNMPSAGSETPSTGEDVSQQPSPG
                     VTYVVVVEDTATIQATETLTPEPPTPQTPPKLTLTKNSNCRLGPSTLYNVVDQIASGA
                     ELSVVGRSEDSEWWQVLNANGRECWVSGENATPNQDFSTLVVGSAPPLPGVPLNFFVA
                     NQICQSAQKKFTVTLSWSSGGGETSFRIYRDGKAIQELKSTRFNFKDVNAPYDKNIVY
                     EIVGLNENGESPRATQIVPACK"
     gene            1047..2183
                     /locus_tag="PKI33_09920"
     CDS             1047..2183
                     /locus_tag="PKI33_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNM37372.1"
                     /translation="MDWFTNPKQNEVKKLITQLADASRRNAAAQELIRLGADSAAPLI
                     DALQTQDLNLLAIYQQILARIPAASPELTKALKSAHPIIRGRVAEVFGLNKDKTAIPA
                     LIEALHGEFFTVRARAALALANIGDRLVISQLLPLLKDQEDEVRMAVCMALGIFKDPS
                     TFDELANVTLDDPIIEVRQAAVKALGDSKNPAAIPFLMEALRDSFWWFEREQSVRVLM
                     NAIEGMGKSVVEPLIEALADREGTVRKLAAKMLGNLRDVRAIEELGMTLYDLHHEVSQ
                     TAAQALAQFGAPAIDVLAEALIHPEASVREHAILGLGQIHDERVIPYLIEMLHDPARE
                     VKRQAIQSLAGIKDRRAIPALQELAGDRTDRELAAFAKEVIAKL"
     gene            complement(2178..>2515)
                     /locus_tag="PKI33_09925"
     CDS             complement(2178..>2515)
                     /locus_tag="PKI33_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37373.1"
                     /translation="KLAELASQYDFSFFEYWASDYAGHKQDMPWAIKQLEVFDGVLKG
                     LLTNWNMENDLVLLTSDHGNMEDLGTRKHTAAHVPLLLFGAKTNRDAFTDVSDLTGVT
                     PAMSSLLGL"
BASE COUNT          663 a          587 c          595 g          670 t
ORIGIN      
        1 ttgttctttc ttcattattg ggaactccga taaggataat acgcaaaaag cgtaattttg
       61 agttgaaaaa tacttaagga aagaacacca aatttcctct ggcgttctgt ttccggtaaa
      121 tcaagtcggg gattatatca gagaaagaac ctccaaattc aaggttgata ggatttgaaa
      181 gtgttaaaaa acacaactct ttgccttagc aaagagttgt gttttttatg gaaatttgtg
      241 ggttatttac aagctggcac aatctgtgtc gcgcgcggag attctccgtt ttcgtttaat
      301 cctacaatct cgtacacaat gttcttatcg tacggggcgt tcacatcttt gaaattgaaa
      361 cgagttgact ttaattcctg aattgctttt ccatcccgat aaatgcggaa gctcgtctcg
      421 ccgccgccgc tgctccaact caacgtcacc gtgaatttct tctgtgccga ctggcaaatc
      481 tgattggcta caaaaaagtt cagcggcaca ccgggcaatg gcggggcgct cccaaccaca
      541 agcgtgctga aatcctgatt gggcgtcgca ttttcgccag atacccaaca ttccctgcca
      601 ttggcattca aaacttgcca ccactcgcta tcttcactgc gcccaaccac cgaaagttct
      661 gcgccagaag caatctgatc cacgacatta tataaagtac tcggacccaa acggcagttt
      721 gaatttttcg taagcgtcaa tttaggcggc gtttgtgggg taggaggttc aggagtaagt
      781 gtttctgtcg cttgaatagt agccgtatct tcaaccacca caacatacgt cacaccgggg
      841 ctgggctgct gggatacatc ttcccccgtg ctgggggttt cactgcctgc acttggcata
      901 ttacatgcga gaattgagag aatcaaaaca acaattgcaa aagaaatatc tcttcttgct
      961 ttcatatgga tctcctctag ttggctaaag tattcttgta attattatac acgccgccca
     1021 gattcaaatt catgtaaaat aaacttatgg attggttcac gaaccccaaa caaaatgaag
     1081 tgaaaaagtt aataactcaa ttggcagatg caagccgacg aaatgccgcc gcacaagaat
     1141 tgatcagact tggcgctgat tctgccgcac cgttgattga cgcgcttcaa acgcaagact
     1201 tgaacctgct cgccatctat cagcagattc tcgcccgaat cccagccgcc tctcccgaat
     1261 tgaccaaagc gctcaaatct gcccatccca tcatccgcgg acgagtagca gaggtatttg
     1321 ggttgaacaa agataaaact gcgataccag ctttaattga agccttgcat ggcgagtttt
     1381 tcactgtccg cgcgagggcg gctttggcac ttgccaatat tggagatcga cttgttattt
     1441 ctcaattact gcctttattg aaagaccaag aggatgaggt tcgtatggct gtgtgtatgg
     1501 cacttggtat tttcaaagac ccttccacgt ttgatgaact cgccaatgta acccttgatg
     1561 accccataat cgaagtgcgg caagcggcag tgaaagcgct aggtgactca aagaatccag
     1621 cagcaattcc ttttcttatg gaagcattac gcgactcgtt ctggtggttt gagcgagaac
     1681 agtctgtgcg tgtgttgatg aatgctatcg aagggatggg caagtcggtc gttgagccgc
     1741 tgattgaagc cttggcagac cgtgaaggga cggttcgtaa attagcagca aaaatgcttg
     1801 gcaatttacg agatgtgcgt gcgattgaag aattgggaat gaccttgtat gacctgcatc
     1861 atgaggtgag tcaaaccgca gcgcaggcat tggcgcaatt tggcgcaccc gctatcgacg
     1921 tgttggcaga ggcgttgatt cacccggaag cttctgttcg agaacatgcc attcttgggc
     1981 tgggacaaat ccatgatgag agggtcatcc catatcttat cgaaatgctt cacgacccag
     2041 ctcgtgaagt gaagcgacaa gccattcaat ctttggctgg cattaaagac cgccgtgcca
     2101 tccctgcctt gcaagagctt gcaggagacc gtacggaccg agagttggct gcttttgcga
     2161 aagaagtgat tgctaaacta taaacctaac aatgaactca ttgccggtgt aaccccggtt
     2221 aagtctgaga cgtctgtgaa ggcgtctcga tttgtttttg ccccaaataa gagaagagga
     2281 acgtgtgcgg cggtgtgttt gcgcgtaccg aggtcttcca tgttgccatg gtcagatgtc
     2341 agcaagacga gatcgttttc catattccaa ttcgtgagca gacctttgag tacgccatcg
     2401 aatacttcaa gctgtttgat cgcccatggc atatcttgct tgtgccccgc gtagtcactt
     2461 gcccaatatt caaagaaaga aaaatcatat tggctggcaa gttcagcaag ttttg
//
LOCUS       DAOREF010000460         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30095_length_2513_cov_3.185110, whole genome shotgun sequence.
ACCESSION   DAOREF010000460 DAOREF010000000
VERSION     DAOREF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30095_length_2513_cov_3.185110"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..663
                     /locus_tag="PKI33_09930"
     CDS             181..663
                     /locus_tag="PKI33_09930"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNM37374.1"
                     /translation="MDTNQAKPMGCFGRGCLFFSLHLIWIILFGVGLYYSFNSFRLVT
                     TGAEVDAIIVDMDADNSDGTTTYSPIYEYIYAGETYRYNSVNSSNIVTYDIGDHTTLL
                     VDPSNPQNARQKSFWELWLMPAIMLPVSLGMGFIMILVQVLAARQGRVANMGNVQINM
                     "
     gene            677..2269
                     /locus_tag="PKI33_09935"
     CDS             677..2269
                     /locus_tag="PKI33_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37375.1"
                     /translation="MSTSKSSSPLLRGLLGLLLLLPACGLCTVDQFWLSLSTLLTSFK
                     NIRISMEGDFVGFKNYIAMFTDRTFPTALGYTTGVIFVHILAAAILPPLLALAVHRFS
                     QRGNRRARLWFTLPLAYFGPALAMFGPAFLRDVWKLTSLHITYLLFDGIVTLAVMSAI
                     GLVVYLAVLRPQQEASSLPTGTLFTTWLVTQLALVAYGLQSLNLLAFLVPGRDGKFML
                     GHMLAQTVRNGYLGLTASVSVFMLVVAAFFGILASVILIARKIQISHRAEEPASFSSN
                     PSIPSWVALALGAVAAFFVTAVPLALSLVKAFGNFASFEFGMFLRISLNTVLPSLLAA
                     LFIQLPVAYLGALGISVVRPFGKSSAWLLLLFSPWLFVTSTPLAFAAFQNLRSADLLD
                     SPLALTPPLLLNVPMLFLLTLFFKGQFDSWQASGKDSSPSLKEALSHWVRPSLPLALF
                     LMVVSGLATAQEVFTPYLTSYSREQQTIALAVAQLAGNFNSGSIAAILALFGIPFFII
                     TFSVLAILQNTYLNKLEITSGT"
     gene            complement(2320..>2513)
                     /locus_tag="PKI33_09940"
     CDS             complement(2320..>2513)
                     /locus_tag="PKI33_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37376.1"
                     /translation="VVEPAKAESLFEGRVLPVITNNERIVSGEEIMPYIKELTRLMYD
                     WQLFQSDACYIRDDGNGVC"
BASE COUNT          513 a          727 c          542 g          731 t
ORIGIN      
        1 cctcagacgg cttcggcaaa ttcttcgctc ccgcccgcgg tttcgccgtg tacaccctca
       61 agtaaaaaca ttcccccagt tgtaggaaca ctgggggaat gtttttttct atcaaaaccc
      121 atacactgat aatgttcctt cgtgtttacc catcttattg aataacgtta gaggtgcaat
      181 atggatacaa atcaagcaaa acccatgggc tgttttgggc gcggctgtct tttcttctct
      241 ttacatctga tctggatcat tttatttggt gtcggtcttt actatagttt caacagcttt
      301 cgcctcgtca ccaccggcgc agaagtggat gccatcatcg tcgatatgga tgccgataat
      361 tcggatggca ccaccaccta ctccccgatc tatgaatata tctatgcagg tgaaacctac
      421 cgctataata gcgttaactc atcgaacatc gtcacctacg atattggcga ccataccacc
      481 ctgctcgtgg acccgtccaa cccgcagaat gcccgtcaga aatccttctg ggagttatgg
      541 ctgatgcccg ccattatgtt gcccgtctct ttgggcatgg ggttcatcat gatcctcgtc
      601 caagtgctgg cagcccgtca gggtcgggtc gccaatatgg gaaatgttca gatcaatatg
      661 taaaacggaa aggtatatgt ccacatccaa atcctcctct ccccttttac gcgggttact
      721 cggtttgctt ttgctcctgc ccgcctgcgg actctgcact gtcgatcagt tctggttgag
      781 tctcagcacc ctcctcacga gttttaagaa cattcgcatc agcatggaag gcgattttgt
      841 cggcttcaag aattacatcg ccatgttcac cgaccgtacc ttccctaccg cattgggcta
      901 caccaccggg gtgatcttcg ttcacatttt ggcggctgcc atcctgcccc ctttgctcgc
      961 acttgccgtt catcgcttca gtcagcgcgg aaaccgccgt gcgcgcttat ggtttacctt
     1021 accattggca tatttcggtc cggcgctagc tatgttcggt cccgccttcc tacgtgacgt
     1081 gtggaagctt acctctctcc atatcaccta tcttttattc gatggcatcg tcacccttgc
     1141 tgtgatgagt gccattggct tggttgtgta tcttgctgtt ttacgccctc aacaagaagc
     1201 cagttctctg cctactggca ccttgttcac gacttggctg gtcacccaat tggcgctggt
     1261 agcatatggt cttcaatcat tgaaccttct ggcattcctc gtccccggca gagatggcaa
     1321 attcatgctc ggtcacatgc ttgcgcaaac cgtccgcaat ggatatttag gtctcaccgc
     1381 ttcggtttct gtctttatgc tggtggtcgc tgccttcttt ggaattctcg ccagcgtgat
     1441 cctgattgca agaaaaattc aaattagcca tcgtgctgaa gagcctgctt ctttttcaag
     1501 caatccatct attccaagtt gggttgctct tgcgcttggt gctgtagcag cgttcttcgt
     1561 cactgcggtt ccacttgctc tgagtttggt gaaagcattt gggaacttcg ctagttttga
     1621 attcggcatg ttcctgcgga tctcactcaa taccgtcctg ccttcattgt tggcggcgct
     1681 cttcattcaa ctccctgtag cctatcttgg tgccttgggc atcagcgttg tgagaccctt
     1741 cggtaaaagc agcgcctggc tcttgctttt gttcagccca tggctctttg tcacttccac
     1801 ccctttggca tttgccgcct ttcaaaacct gcgctcggca gacctgctcg attctccgtt
     1861 ggcactcaca ccacctttgc tgttgaatgt ccccatgctt ttcctgctca cccttttctt
     1921 taaggggcag tttgatagct ggcaggcatc tggaaaagac agttctccca gcctgaaaga
     1981 agccctctca cattgggtgc gtccctctct accactggct ttgttcctga tggtggtctc
     2041 cgggcttgcc actgcacagg aagtattcac gccctatctc accagctact cacgtgaaca
     2101 acaaacgatc gcacttgccg tggctcaact ggcagggaat ttcaactcag gcagcattgc
     2161 cgccatcctt gccttgtttg gcattccctt ctttatcatc acttttagtg tgctcgccat
     2221 tctccaaaac acctacttga acaagctcga aatcacgagc ggaacttaag cataaaaaag
     2281 caccccgttg atataaggtc aacggggtgc tttgcttcat caacaaacgc cattcccatc
     2341 atcgcggata taacacgcat cgctttggaa caattgccag tcatacatca gccgggtcaa
     2401 ctctttgatg tagggcataa tttcttctcc actcacgatc cgctcgttgt ttgtgatcac
     2461 cggcaaaaca cgcccctcaa aaagagactc tgccttggca ggctccacca caa
//
LOCUS       DAOREF010000461         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30110_length_2512_cov_5.943834, whole genome shotgun sequence.
ACCESSION   DAOREF010000461 DAOREF010000000
VERSION     DAOREF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30110_length_2512_cov_5.943834"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKI33_09945"
     CDS             <1..474
                     /locus_tag="PKI33_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNM37377.1"
                     /translation="GEGAAAARAAGADFVADDDESLKKIEGGMLDFDVAIATPDAMGK
                     VGRLGRVLGPRGLMPNPKAGTVVGASDMDRAIKEAKAGRVEYRLDKSNNIHVSIGKAS
                     FDATKLYENYVALMDAINKSRPTGAKGNFVKRITVASTMGPGVKVDPNEHIDGEG"
     gene            complement(480..608)
                     /locus_tag="PKI33_09950"
     CDS             complement(480..608)
                     /locus_tag="PKI33_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37378.1"
                     /translation="MLKYATGSPPEQDIKFRLGHVLRTTICRTPAVIGEVGLGIIG"
     gene            660..1223
                     /gene="rplJ"
                     /locus_tag="PKI33_09955"
     CDS             660..1223
                     /gene="rplJ"
                     /locus_tag="PKI33_09955"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNM37379.1"
                     /translation="MAKTFYLKGGEFPLAISKQRKEEVLAQYNDWVKRSQAVILVEYS
                     GANMKALDGLRAKIRESGGEFHVVKNTLARRVFADNGMEVPGDMLVKSTAVSFAFSDP
                     ASTAKALTDATKGSEFVKVKGGFMAGKPLNAAQVKALSDLPPLPVMRATLLGVLQAPA
                     GKLVRTLAEPARGLAAVIKARSEQAAA"
     gene            1298..1681
                     /gene="rplL"
                     /locus_tag="PKI33_09960"
     CDS             1298..1681
                     /gene="rplL"
                     /locus_tag="PKI33_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNM37380.1"
                     /translation="MSDKLAKLVDELSTLSVLEAADLVKMLEEKWGVSAAAPVAVAAV
                     GGGAAAAAEPVEEKTEFDVIIKDAGAKKIDVIKVVRQLTSLGLGEAKALAEAAGSKVL
                     TGVGKDAAMDAKKKLEEAGAQVVVE"
     gene            complement(1748..2053)
                     /locus_tag="PKI33_09965"
     CDS             complement(1748..2053)
                     /locus_tag="PKI33_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37381.1"
                     /translation="MYGTIARFRVRKDVNKEEFKQKMDEFGSAIIPGWIADYIFQTDA
                     NSNEYFLVALFKDKATYQANADSAEQHERYLIFRSFLEDDPEWNDGTIVSATGPGSK"
     gene            complement(2162..>2512)
                     /locus_tag="PKI33_09970"
     CDS             complement(2162..>2512)
                     /locus_tag="PKI33_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNM37382.1"
                     /translation="AEDTVDIATSLMPSKEKGKDLFALHVQGDSMIDAMINDGDIVVL
                     KPAQEARNGEMVAVWLSDKNETTLKYFYKEKDGYRLQPANPTMKPIMVKKNEPLEIKG
                     KVVMVIRKMERLAA"
BASE COUNT          566 a          617 c          665 g          664 t
ORIGIN      
        1 ggtgaaggcg ctgctgctgc tcgtgccgct ggtgcggatt ttgttgcgga tgatgatgaa
       61 tctttgaaga agatcgaagg cggcatgctc gacttcgatg tggcgattgc tacccccgat
      121 gcgatgggta aggttggtcg cttgggtcgt gtgcttggtc cgcgcggctt gatgccgaac
      181 cccaaggctg gtaccgttgt tggcgcttct gatatggatc gcgccatcaa ggaagccaag
      241 gctggtcgtg tagaataccg ccttgataag agcaataaca tccacgtttc tatcggtaaa
      301 gcttctttcg atgcaaccaa actgtatgaa aactacgtcg ctctgatgga cgcgatcaac
      361 aagtctcgcc cgaccggtgc gaaaggcaat tttgtgaagc gcattaccgt tgcttccacc
      421 atgggacccg gcgtcaaggt cgatcccaac gaacatattg atggagaagg ataagcactt
      481 tatcctataa ttcctaaccc cacttcgcca atgacggcgg gtgttctgca aatggtcgtg
      541 cggagtacgt gacccaagcg gaacttaata tcctgctcag gtggagaccc agtagcatat
      601 ttcagcacct ccgtgctgtt ctcccgtgag ggagtgcgaa aaagtctttg cctgcgagag
      661 tggcaaagac tttttatttg aaaggaggtg aattcccttt ggcaatttca aagcaacgca
      721 aggaagaagt tttggctcag tataatgact gggtcaagcg cagccaggcg gtgattctcg
      781 tggagtactc tggcgctaac atgaaagcgc tcgatggtct ccgtgcgaag atccgtgaat
      841 ctggtggtga attccatgtt gtcaagaata cgttggctcg ccgcgttttc gctgacaatg
      901 gcatggaagt ccccggtgac atgctcgtca agtctaccgc tgtctcgttc gcgttctcag
      961 atcctgcttc caccgcaaaa gccctgaccg atgctacaaa aggtagcgag tttgtgaagg
     1021 taaagggtgg tttcatggct ggtaagccgt tgaacgctgc tcaggtgaag gctttgtctg
     1081 acctgccgcc gctgcccgtt atgcgcgcaa cattgctcgg cgttttgcaa gccccggctg
     1141 gcaaactcgt ccgcacgctc gcagaacccg ctcgcggatt ggctgccgtc atcaaggcac
     1201 gttccgaaca ggctgctgcc tagttttatc caagcgagtt ccaaaactcg caccgttaat
     1261 caaaattatc taattactta gcaaggagtg ctaaacaatg tctgacaagc tcgctaaact
     1321 cgtggatgaa ctttccaccc tttccgttct cgaggctgct gacctcgtaa agatgctcga
     1381 agaaaaatgg ggcgtttctg ccgctgctcc ggtcgctgtt gctgccgttg gtggtggtgc
     1441 tgccgccgct gctgaacccg ttgaagaaaa gaccgaattc gatgtgatca tcaaggacgc
     1501 tggtgccaag aagatcgacg tcatcaaggt cgtccgccag ttgacctccc tcggtctcgg
     1561 cgaagccaag gctcttgccg aagctgctgg ttccaaggtt ctcaccggtg tgggcaaaga
     1621 tgccgccatg gacgccaaga agaagctcga agaagctggc gctcaggtcg tcgtcgagta
     1681 atctactact cggaacaaaa aaggactgtc cgatttcggg cagtcctttt ttgattcatg
     1741 cgcttcttta cttggaccct ggaccggttg ctgaaacaat cgtcccatca ttccattcgg
     1801 ggtcgtcttc cagaaacgaa cggaagatca aataccgttc atgctgttct gcagaatcag
     1861 catttgcttg ataggttgct ttgtctttga agagtgctac aaggaagtat tcatttgagt
     1921 tagcatcggt ttggaagata tagtctgcga tccagccggg gatgatggct gagccgaact
     1981 catccatttt ttgcttaaat tcctccttgt ttacatcttt tctgacacga aaacgagcga
     2041 tggttccgta cataagtcac ctcttttctt gtatcgataa gacgctttca ataattaata
     2101 cacctcaaga atcaaaaaga agcggcatat atgccgcttc tttttgatag tttataaatg
     2161 attaggctgc caagcgttcc attttgcgga tgaccatgac aactttgcct ttgatctcca
     2221 agggttcatt ctttttgacc ataataggct tcatggtggg gttggcgggc tgcaggcgat
     2281 aaccatcctt ttccttgtag aagtacttca gagtggtttc gttcttgtct gagagccaga
     2341 cggcgaccat ttcaccgtta cgggcctcct gtgccggttt gaggaccacg atatcgccgt
     2401 cgttgatcat ggcgtcgatc atggagtctc cttgcacgtg aagggcgaag aggtctttgc
     2461 ctttttcctt tgaaggcatc agagaggtgg caatatccac ggtgtcttct gc
//
LOCUS       DAOREF010000462         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30112_length_2512_cov_4.911274, whole genome shotgun sequence.
ACCESSION   DAOREF010000462 DAOREF010000000
VERSION     DAOREF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30112_length_2512_cov_4.911274"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="mraY"
                     /locus_tag="PKI33_09975"
     CDS             <1..884
                     /gene="mraY"
                     /locus_tag="PKI33_09975"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNM37383.1"
                     /translation="IEQPEGHRVKMGTPTMGGVMFIVPVLLLSGLLNAVALIGVTASG
                     VGRSVLLPMVVMVGFAILGAVDDWEGIHGKRKGDGMRARTKFAFQVILALATAYVLKY
                     FIKAPDLYLPGYFFEIQIGWTYVPIAAFIIVAESNAVNFTDGLDGLAGLIAATAIAAF
                     GGIALMQEQIYLARFCFILVGALFGFLWFNVHPAQLIMGDTGSMALGSVLAVIALMTG
                     QWPMLLVIAIIPLAEMMSVVIQVTYFRVTGGKRFFKMTPIHHHFELLGWSETQIVQRF
                     WLIGLMAALVGIGLSQV"
     gene            886..2259
                     /gene="murD"
                     /locus_tag="PKI33_09980"
     CDS             886..2259
                     /gene="murD"
                     /locus_tag="PKI33_09980"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNM37384.1"
                     /translation="MTNWTGKHVLILGAARQGLALARWLARHGANVTLNDSRREEDLI
                     TAKKSLADVNVTWAVGGHPLELLDTTDVLCISGGVPLTLPIVEEAIKRDIPLSNDSQI
                     FMEVVSCKTVGITGSAGKTTTTTLVGNMAKLAKGDRAFVGGNIGDPLINYVDDMQADD
                     LAILELSSFQLEQMTISPNIAAILNITPNHLDRHGTMEAYTNAKAHILDFQSAKDNAI
                     LGRDDKGAWGLKNRVKGNLLSFSLQDLDEGLNGTYLHDGLLSLRDGFAYVPLLMREKV
                     QLRGDHNIANVLAAFAIGHAAGFKLDDMLEAVEEFRGVPHRLELVRELNGARWYNDSI
                     ATAPERAAAAVHAFSEPIVLMLGGRDKNLPWGDLAKLIHERVDHVVLFGEAGEMIQNA
                     ITAYSGVGTVDVHRAMTLKEAVTLAADVTSAGDVVLLSPGGTSFDEFKDFAERGEAFR
                     KWVSELS"
     gene            2238..>2512
                     /locus_tag="PKI33_09985"
     CDS             2238..>2512
                     /locus_tag="PKI33_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNM37385.1"
                     /translation="MGIGTFVRETSPQDRPRAGGFDMPLLVIVVALIVFGLVMLYSAS
                     WDFSRAAYNGDAMYMFNRQLMWLGLGTVAALILAFIDYHRWRQFIVPA"
BASE COUNT          548 a          538 c          700 g          726 t
ORIGIN      
        1 gtattgaaca gccggaaggt caccgtgtga aaatgggtac cccaactatg ggcggggtga
       61 tgtttattgt gccggtcttg cttcttagcg gtttgctgaa tgcggttgcc ttgattggtg
      121 taaccgccag tggggtgggt cgctctgtgt tgttgcccat ggttgtaatg gttggttttg
      181 ccatcctagg tgcagtggac gattgggaag gtattcacgg caaacgcaaa ggcgatggga
      241 tgcgcgcccg cacgaaattt gcctttcaag tgattcttgc cttagcaaca gcctacgtgt
      301 tgaaatattt tattaaagca cccgacctgt atttaccggg ttatttcttc gaaattcaaa
      361 tcggttggac ctatgtgccc attgctgcat tcattattgt tgctgagtca aatgcagtca
      421 atttcacaga tgggcttgat ggtttagctg ggttgattgc ggcgactgcc attgccgctt
      481 tcggtggaat cgctcttatg caagagcaga tctatctggc acgtttttgt tttatcctcg
      541 ttggagcctt gttcggcttt ttatggttta acgttcaccc tgcacaattg attatgggcg
      601 atacaggctc aatggcattg ggttctgtac tggctgttat cgcgttgatg accgggcagt
      661 ggcctatgtt gttggtgatc gcaattattc ctttggctga aatgatgagc gttgtcattc
      721 aggtgacata cttccgcgtg acaggcggaa agcgcttttt caaaatgact cccatccatc
      781 atcattttga gttgctgggt tggagtgaaa cgcaaattgt ccaacgcttt tggctcatcg
      841 gcttgatggc agccttggtt ggaatcggat tatcgcaagt gtagtatgac gaattggact
      901 ggtaagcacg ttctcattct tggagccgcc cggcagggac tcgcccttgc tcgttggctt
      961 gcacgtcacg gcgcaaacgt gaccttgaat gattcgcgtc gcgaagaaga ccttatcact
     1021 gcgaaaaaat ctctcgctga tgtgaatgtg acttgggcag tgggtgggca tccgcttgag
     1081 ttattggata caactgatgt attgtgtatc tctggtggcg taccgttgac cttgcctatt
     1141 gtggaagaag ctattaagcg tgatattccg ctttcgaatg actcacaaat cttcatggaa
     1201 gtggtttcat gcaaaacggt tggcatcact ggttcggcag gtaagacgac cacaacaact
     1261 ctcgttggca acatggcaaa acttgcaaaa ggcgaccgtg cttttgtggg tggcaatatt
     1321 ggcgatccac tcatcaacta tgtggatgac atgcaagctg atgatttagc catattggaa
     1381 ctttcttcct tccaacttga gcagatgact atttcaccga acattgcggc gattttgaac
     1441 atcacaccga atcacttgga tcgccacggt acgatggaag cctataccaa tgccaaggcg
     1501 catatcttgg attttcagtc tgcgaaggat aacgcgatct tagggcgcga cgataaaggc
     1561 gcttggggtt tgaagaaccg tgtgaaaggg aatttacttt cgttcagctt acaggaccta
     1621 gacgaaggct tgaacggtac atatcttcac gatggtttgc ttagcttacg agatgggttt
     1681 gcttacgtcc cattgttgat gcgtgaaaaa gttcagctac gtggcgacca taacattgca
     1741 aatgtgttgg ctgcttttgc cattgggcac gcggctggtt tcaagttgga tgatatgctc
     1801 gaagctgtgg aagaattccg tggtgtgccg catcgcttgg agttggtgcg cgaattgaat
     1861 ggtgcacgct ggtataacga ctcgattgct acagctcccg agcgcgcagc agcagccgtc
     1921 catgcattta gtgagccgat cgtgctcatg cttggtggac gtgacaaaaa tcttccttgg
     1981 ggcgatcttg ccaaacttat ccacgaacgt gtggaccatg tggtgctctt tggtgaggcg
     2041 ggggagatga ttcagaacgc aattacagcc tacagcggag tcggaaccgt ggacgttcat
     2101 cgggcaatga ctctcaaaga ggcagttaca ctggctgcgg atgttacatc cgcaggggat
     2161 gtcgtccttt tatcaccggg cggcacgagc tttgatgagt ttaaggattt tgcggagaga
     2221 ggagaagcgt ttagaaaatg ggtatcggaa ctttcgtaag agaaacttca cctcaagacc
     2281 gtccgcgtgc gggcggcttc gacatgccac tcttggtgat cgtggtggca ttgatcgtat
     2341 ttggtttggt gatgttgtac tcagcttctt gggacttttc acgcgctgcc tataacggcg
     2401 atgcgatgta tatgttcaat cgtcagttga tgtggcttgg gcttggaacc gtagccgcgc
     2461 tgattttagc gtttatcgat tatcaccgtt ggcgccaatt cattgtgcct gc
//
LOCUS       DAOREF010000463         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30192_length_2509_cov_4.966993, whole genome shotgun sequence.
ACCESSION   DAOREF010000463 DAOREF010000000
VERSION     DAOREF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30192_length_2509_cov_4.966993"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKI33_09990"
     CDS             <1..239
                     /locus_tag="PKI33_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNM37386.1"
                     /translation="IGIRTDGRKMMEVANKIAKFDEVVYIVATSGRYDLMIEVFCADH
                     EDLFNFMAEKLAKVDGVRETESFIYLKIAKEIYF"
     gene            564..1763
                     /locus_tag="PKI33_09995"
     CDS             564..1763
                     /locus_tag="PKI33_09995"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNM37387.1"
                     /translation="MKVLLVGVGGVGEAIAAIAKPREWMELMVLADYNVKRAEEVQKK
                     LGDPKRFPVEFIDAGNQKNIEDLAKKHQVDLIMNSVDPIFNQQIFDAAYNAGVKYMDM
                     AMTLSEPHPTDPFNKPGVKLGDYQFEKAKEWEKKGLLALVGFGVEPGMADVFARYAAD
                     HLFDEIEEVGIRDGANITIEGYDFAPNFSIWTTIEECLNPPVIWQNGDWHTTAPFSEP
                     EVFDFPEIGPVEVVNVEHEEVLLVPRWVKAKRVTFKYGLGNEFIEVLKTLHKLGLDNK
                     NKINVKGVQVAPRDVVAACLPDPAHLGDKMKGKTCAGTWVKGTKDGKKKEVYLYQVAD
                     NEECMKTWGCQAVVAQTAFTTVIAMDLLEHGAWKGTGVLGPEAFEPTVFMEKMADYGF
                     PYGIKNM"
     gene            1982..>2509
                     /locus_tag="PKI33_10000"
     CDS             1982..>2509
                     /locus_tag="PKI33_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNM37388.1"
                     /translation="MPNGYNGKILHVDLTKGSLTVEEPNEAFYRKYLGGSAMGMHYIL
                     RDMPKGADPLGPENVLTLFTGVTTGAAISGQSRLNANAKSPISGGIGDSQSGGFFPAE
                     LKFAGFDGIVVKGKSPKPVYLCIIDGKFELRDASHLLGKLSGEVDDIIHKEVDPKAEI
                     LQHGIGAENGVLYSSL"
BASE COUNT          663 a          516 c          700 g          630 t
ORIGIN      
        1 tgatcggtat tcgtaccgat ggacgcaaaa tgatggaagt ggcgaacaag atcgcgaaat
       61 ttgacgaagt cgtgtacatc gttgccacca gcggtcgcta tgacctgatg atcgaagtct
      121 tttgtgccga ccatgaggat ttgttcaatt tcatggctga aaaactggca aaagtggatg
      181 gcgttcgcga aaccgaatcc ttcatttact tgaagatcgc caaagagatt tatttctaat
      241 gggaattccg cttcagtatg gaccttatgc ctgctgcagc gttccaaaac tttgtctgca
      301 ctggaaacga atccagaacg cagagccaac tgaaaggttc taatgaaagc cctagctcgt
      361 gtactaaaga ctgtggggga taccttcttg aaggtttata tgacttcact catgatttcc
      421 atttttccag gaaatcaagc cagcgatatg aatcgcaatt ggtagcttca gtctgtcccg
      481 ccgcctttac tgcggcggga ttcttttttt cagtcaatct aactcgctgg tgcgggttat
      541 aaaactaaac ggagaatcat ctcatgaaag tattgctagt tggagtgggt ggagttgggg
      601 aagccatagc cgccattgcc aagccgcgcg agtggatgga gttgatggtg ctggcagatt
      661 acaacgtcaa acgagcagaa gaagtgcaaa agaagctggg agaccctaaa cgcttcccgg
      721 tcgaattcat tgatgctggc aaccagaaaa atatcgaaga cttggcaaag aaacaccaag
      781 tggatctcat catgaattca gtagacccga tcttcaacca acagatcttc gatgccgcct
      841 acaatgctgg cgtgaaatat atggacatgg cgatgaccct ctccgagccg cacccaaccg
      901 acccgttcaa taaaccgggt gttaagttgg gcgattatca atttgagaaa gccaaagagt
      961 gggagaagaa aggtttgttg gcgcttgttg ggtttggcgt ggaacctggc atggcagatg
     1021 tctttgctcg ctatgcagct gaccatctct ttgacgagat cgaagaagtg ggcattcgcg
     1081 atggcgctaa tatcaccatt gaaggctatg attttgcgcc gaatttttca atttggacaa
     1141 ctattgaaga atgtttgaat ccgcccgtca tctggcaaaa cggagattgg cataccacgg
     1201 ctccgttctc tgagcctgaa gtgttcgact tccctgaaat tggacccgtt gaagtcgtca
     1261 acgtggagca tgaggaagta ttgctcgtgc cgcgctgggt gaaggcaaag cgtgtgacct
     1321 tcaagtatgg gttggggaat gaatttattg aagtgttgaa aacccttcat aaacttggct
     1381 tggataataa gaacaagatc aatgtgaaag gcgtgcaagt tgcgccgcga gatgtggtgg
     1441 cggcttgttt accagaccca gctcacttgg gtgacaagat gaaaggcaag acatgtgccg
     1501 gaacctgggt aaaaggcaca aaagatggga agaagaaaga agtatatctg tatcaggttg
     1561 cggataacga agaatgcatg aagacttggg ggtgccaggc agtggtggct caaacggcat
     1621 ttaccactgt gattgctatg gatttgctgg aacatggcgc ttggaagggc acaggtgtgc
     1681 tcggacctga ggcgtttgag ccgactgtct tcatggaaaa aatggcagat tacggcttcc
     1741 catacggcat taagaatatg tagtttataa aagggcgact ctttacggga cgcccttttg
     1801 cttcgggtgt gaaaggttcg agaaagcggt atccgctgtc agttaggaag gaagcgcggc
     1861 ggggagtgta ggaaaaaact taatcgaagc ggttaagctg tcgctttcgg aaactgcctg
     1921 ttgtgagaag tcagacccgc ttgttataat taccatcatc aattcttacg gaggagtaac
     1981 catgccaaac ggttataacg gaaagatttt gcacgttgat ctgacgaagg gctctctgac
     2041 ggtggaggag ccgaatgagg ctttttaccg caagtattta ggcgggagcg caatgggaat
     2101 gcactacatc ttgcgcgata tgccgaaggg cgctgacccg cttggacctg aaaatgtttt
     2161 gaccttgttt accggtgtta caactggggc ggcaatctca ggtcagagcc gtttgaatgc
     2221 aaatgccaag tcgccgatca gtggcggtat tggtgattcg cagagcggtg ggttcttccc
     2281 ggctgagttg aagtttgccg gttttgatgg cattgttgtg aaggggaagt cacccaagcc
     2341 tgtatatctc tgcattattg atggaaaatt tgaattacgc gacgcctcgc atttgctggg
     2401 caagctttcg ggcgaagtgg acgatatcat ccacaaagaa gtggacccga aggctgagat
     2461 ccttcagcat gggattggcg ctgaaaatgg ggtactgtat tcatccctc
//
LOCUS       DAOREF010000464         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30310_length_2504_cov_3.632911, whole genome shotgun sequence.
ACCESSION   DAOREF010000464 DAOREF010000000
VERSION     DAOREF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30310_length_2504_cov_3.632911"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..174
                     /locus_tag="PKI33_10005"
     CDS             7..174
                     /locus_tag="PKI33_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37389.1"
                     /translation="MVLQGETPNPINIPSGCRFHPRCPAAFDACKLSDPPMTTLSKSH
                     QAACLLVTNTK"
     gene            175..948
                     /locus_tag="PKI33_10010"
     CDS             175..948
                     /locus_tag="PKI33_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNM37390.1"
                     /translation="MDITSLQNPRVKHIVKLREDKKQRQKDRLILVEGYDELTLALAC
                     GFEPQTLLSAPELVSQTLTVSNAENLTVSRAVFEKISYRDNPDGWLGLFPMPTSSLDE
                     LKLSENPLVIVAESVEKPGNLGAILRTADAAHVDAVLVCDPRVDTWNPNTIRASRGAV
                     FAVPVVEVKSAEALSWLRSRKMRVLAATPSADAMYTDVDMQGPLAIAVGTEDEGLTDF
                     WMQNSDLRVKIPMTGKVNSLNVSIATALMTYEAVRQRAK"
     gene            945..>2504
                     /locus_tag="PKI33_10015"
     CDS             945..>2504
                     /locus_tag="PKI33_10015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM37391.1"
                     /translation="MSSKLVRQNSRSETAVFANLGRDLASTTEARSAAEIILNAADSL
                     IGWDAAYLILYDPAIGGVPRPLLTIDTINGNHVEQKNAMPQKPSENMLKAIAHGGFMS
                     HYDEPFDIGVTASFGDRSKRTLSQLFVPVVSGSRTIGVLSIQSYQKYLYKDEQLELLK
                     DLASHCAGALERIWAQEAVNDLVERLKVLHAAVNAINASLEMERVCQVVYETVKQVMP
                     CDDFVIDGYDKDTNEIVPIYAIEYPGKRVFTNRYLADHGMAGEIVLSQKSLLFNSVEE
                     MDNSGIKFELYGTHEDDPSQSILAVPMTLHGDMYGMVSAQSYNTNAYNRDHLYLLEVL
                     ASHAAIAIENARLFDSIQNLANTDSLTKILNRRRFFELAEQEFVRAEQFNIPFSVIMI
                     DVDDFKQFNDKHGHKIGDAVLILAAETLKSSLRTDDILGRIGGEEFVAALPNTPIPFA
                     MEVASRLRQAIQDTNFSARIRHEMPDLLITVSIGVAEYASNCKTLDTLLDRADQAMYN
                     SKNSGRNQVRAW"
BASE COUNT          672 a          576 c          587 g          669 t
ORIGIN      
        1 gaacgtattg tccttcaagg tgaaacgccc aaccccatca atattccttc gggctgtcgt
       61 tttcatccgc ggtgcccggc agcctttgat gcctgcaaac tcagcgaccc gcccatgaca
      121 accctttcaa aatcacatca ggcagcttgt ctgttagtaa cgaatacaaa ataaatggat
      181 attacaagtt tacaaaaccc gcgcgtcaag catattgtca aattgcgtga ggataaaaag
      241 cagcgccaaa aggatcgctt gattttggtg gaaggctacg atgaattgac tctcgcgctt
      301 gcttgtggat tcgaaccgca aacgcttctt tctgcgcccg aacttgttag ccaaacccta
      361 actgtttcca acgctgaaaa tttgaccgtt agccgtgccg tctttgaaaa gatttcctac
      421 cgtgataacc ccgatggttg gcttgggctt ttccctatgc caacatccag cttggatgaa
      481 ttgaagcttt cagaaaatcc gctagtgatt gtcgctgagt ctgtagagaa gcctggtaat
      541 cttggcgcca tccttcgcac tgccgatgcc gcgcatgtag atgcagttct cgtctgcgac
      601 ccgcgtgtgg atacgtggaa tccaaacacc attcgcgcca gccgcggagc agtctttgcc
      661 gtacctgttg tcgaggtgaa atcggctgaa gcgctgagtt ggttgaggtc ccgtaagatg
      721 cgtgtattgg ctgcaactcc atccgcagat gcaatgtaca cagatgttga tatgcaaggt
      781 ccgcttgcca ttgcggttgg gacagaagat gaaggtctca ccgatttctg gatgcagaat
      841 tcagacctgc gtgtaaaaat tcccatgacg ggaaaagtaa actccttgaa tgtttcgatt
      901 gccactgcac taatgactta tgaggcagta cgacaaagag caaaatgagc agcaagctgg
      961 tacgtcagaa ttctcgaagt gaaacagccg tttttgcaaa cttggggcgt gatctagcat
     1021 cgacgactga agcaaggtct gcagcagaga tcatattaaa tgccgcagat agccttattg
     1081 gttgggatgc cgcttatctt atcctttatg accctgccat cggtggtgtt ccacgcccat
     1141 tgcttacaat cgacactatt aatggaaacc atgtagaaca aaagaatgcc atgccgcaaa
     1201 agcccagtga aaatatgctg aaggcaattg cgcacggcgg ctttatgtct cactatgacg
     1261 agccttttga tattggagta acggcttcat tcggagatcg aagtaaaagg actttatctc
     1321 aattgtttgt gccggtcgtc agcggttcgc ggacaattgg tgtgctttcc attcagagct
     1381 atcaaaagta tctttataaa gatgagcaac ttgaactatt gaaagatcta gcaagccatt
     1441 gtgctggagc cttggaacgt atttgggcgc aagaggctgt aaatgattta gtcgaacgct
     1501 tgaaagttct tcatgccgcc gttaacgcca tcaacgcaag ccttgaaatg gaacgggttt
     1561 gccaggttgt atacgagacc gttaaacaag tcatgccgtg cgatgacttt gtgattgatg
     1621 gctatgataa agatacgaac gagattgtgc cgatctatgc tattgaatac cccggtaagc
     1681 gagtctttac gaatcgatat cttgctgacc atggtatggc tggagagatt gttctttccc
     1741 aaaaatcact gctttttaac agcgttgaag aaatggacaa tagcggcatc aagtttgaac
     1801 tttatggcac gcatgaagat gacccgagtc agtctatttt ggctgtgcca atgaccttgc
     1861 atggtgatat gtacggtatg gtctcggcac aatcgtacaa tacaaatgct tataaccgag
     1921 accatttata tttattggaa gttcttgcgt ctcatgctgc aattgccatc gaaaatgccc
     1981 gtttatttga ttccatccaa aacctggcaa atacagactc gctcacgaaa atactcaatc
     2041 gccgtcgctt ttttgaatta gctgaacagg aatttgtccg tgctgaacaa ttcaatattc
     2101 ctttttcagt catcatgatc gatgtggatg atttcaaaca gttcaacgac aaacacgggc
     2161 acaaaatagg ggatgcggtt ttgatcctcg ctgctgaaac gctaaagtct tctctacgta
     2221 ccgatgacat tcttgggcgt attggcggag aagaattcgt tgccgctctt cccaatacac
     2281 caatcccctt tgcgatggag gttgcgtctc gtttgcggca agcgattcaa gacacaaatt
     2341 tttcagcgcg tattcggcat gaaatgccag acttattaat cacggttagt ataggcgttg
     2401 cagaatatgc aagcaactgt aagactctcg acaccctcct agaccgagct gaccaagcaa
     2461 tgtacaactc gaaaaattca ggtcgaaacc aagtccgcgc ttgg
//
LOCUS       DAOREF010000465         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30360_length_2502_cov_2.591336, whole genome shotgun sequence.
ACCESSION   DAOREF010000465 DAOREF010000000
VERSION     DAOREF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30360_length_2502_cov_2.591336"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2502
                     /locus_tag="PKI33_10020"
     CDS             <1..>2502
                     /locus_tag="PKI33_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM37392.1"
                     /translation="HLYLLKNALPQVLQPALIISTQTVLFQFPANTWVDVLAFEQNPT
                     SLQDMEKLTLLYTADLAQGVEIDDVLFSRREELRNRFVHLLKMLAQTYFNNTNYETSI
                     HWVRKLLTQDPWDEEALRLYMTSDAILGNRTAALSTYKSFEKKLRLEMNIQPMPETMA
                     LYRDILHNHLLRSTTPSKTEGELFISRQSEMDVLNTAMHEFRQHGSFVIINGPAGVGK
                     TSLVREAVRRFLNVSDIKLLWGDCQPTGEEQPYSIWQQIFNAAVPLLVRQHDVAQEWL
                     NHLLPLIPDLSLLRPDLFLPSRPDGIELRAAMRQTLIALAGNRPLIVVIEDAHWMDIP
                     SLEFLKELTETSPNIPILFILTHRIDDVALPLLDLKRTLRRNRNVVDIYLQPFTETET
                     RQYLETTLGTKHLSAELIDDLMHYAGGLPLLLHEAAQTLKQQGTSDKGLLSLREAFIL
                     RLGSLSPAVREMVEMAAIIGFSLTDVELRRSLGWATEAYAAAMDILQAERFLVEPYST
                     STDEYAFSHRLVHEIILGQIPTERSSALHIRAAQALEQIHSEELGFAAEIALHYQQAG
                     QFVQAANYWADHASESTDLAAFDAGLAAINQAEAILKDASLSAQEVRAKLALQRGVIV
                     FYQGKSEEAFSLLEKAVERSKIFPPLYAHALSMQAYAFHTHDRNELAFQAASQALDLS
                     MATKDVSNAVRALNIRAVSALMLGKTRNALDDLQQALALLEQHNLSASSQTVQSMYHL
                     GTALVFSQDYAQAHAMLARTVELSKKGGLRRLEAAALTMLGQIALNCGQYKQAVELYD
                     RSIEVAGASYLPGMWGKFAGRGWANLRMGNLLAAHA"
BASE COUNT          650 a          610 c          576 g          666 t
ORIGIN      
        1 gcatctctat ctcctaaaaa atgctcttcc acaagtactg caaccagcgc tgataatctc
       61 tacacaaacc gttttatttc aattccctgc caatacctgg gtggatgtgc tcgcatttga
      121 acagaatcca acttctttgc aggatatgga aaaactcacc ttgctgtata cagcagacct
      181 tgcccaaggg gtagaaattg acgatgtttt attttctcgg cgtgaagaac ttcgcaatcg
      241 tttcgttcat ctgttgaaaa tgctggcaca gacttacttt aacaatacca actacgagac
      301 atccattcat tgggtaagaa agctattgac acaagaccca tgggatgaag aagccctgcg
      361 tttgtatatg acctcggatg ccatccttgg caatcgcacc gctgcactct caacctataa
      421 atcttttgaa aaaaagctgc ggctcgaaat gaacatccaa cccatgccag agacgatggc
      481 gctatatcgc gatattttgc ataaccatct tctccgatcc acaacccctt caaaaacaga
      541 aggggagcta ttcataagcc gccaaagtga aatggatgta ttgaacactg cgatgcatga
      601 atttaggcag catggcagct ttgtaatcat taatggaccc gctggtgttg ggaagacctc
      661 gttagttcgt gaagcggttc ggcgcttttt gaacgtctcg gacattaagt tgctttgggg
      721 tgattgtcag cctacagggg aggaacaacc ctattcgatc tggcagcaaa tttttaatgc
      781 agcagtcccc ttgcttgtgc gccaacacga tgtagcgcaa gagtggctaa atcatttatt
      841 gccgctcatt ccagatctga gcctgctacg acccgatctg ttcttgccct cccgtccaga
      901 tggtatcgaa ttgcgcgctg caatgcggca aactttaata gcgctggcag gtaaccgtcc
      961 gctcattgtg gtgattgaag atgcacattg gatggatatt ccgtctttgg aattcttgaa
     1021 agaactcact gaaacttccc caaacattcc tattcttttt attcttacgc atcgcataga
     1081 cgatgttgct ttacctttac ttgatcttaa gcggacatta cgccgcaacc ggaatgtagt
     1141 ggatatctat ttgcagccgt tcacagaaac agagacacga caatatcttg aaacgacttt
     1201 gggaacaaag catctttctg ccgagcttat agacgacctt atgcattacg ctggcggatt
     1261 gcctttgtta ttgcacgaag cagctcaaac tctgaaacaa caaggaacct cagacaaagg
     1321 tctcctctct ttacgtgagg cttttatcct gcgattgggc agtctctcgc ctgctgttcg
     1381 tgaaatggta gagatggctg ctatcatcgg cttttcattg accgatgtcg aattgcgtcg
     1441 gtctttaggt tgggcaactg aagcgtacgc agcggcaatg gacattttac aagccgaacg
     1501 cttccttgta gaaccttatt caaccagcac agatgaatat gcattttctc accgcctggt
     1561 gcatgagatt attttagggc agatccccac agagcggagc tcagctttac atattcgcgc
     1621 tgcacaggcg ctggaacaga tccactcaga agagcttggt tttgcggcag agatcgccct
     1681 gcactatcag caagccggtc agttcgttca ggctgccaac tattgggcag atcacgccag
     1741 cgaaagcaca gacctggcag cgtttgatgc tggtttggct gctattaacc aagcggaagc
     1801 gatactcaaa gacgcaagtt taagtgctca agaggtgcgc gctaaactgg ctcttcagcg
     1861 cggggtgatc gtgttttatc agggaaaaag cgaagaggcg ttttctttgc ttgagaaagc
     1921 agtggaacga tccaagatct tcccgccttt gtatgcacat gctctttcta tgcaagcata
     1981 tgcctttcat acgcatgatc gcaatgagct tgcatttcaa gccgcttctc aagcactcga
     2041 cctttccatg gcaaccaaag atgtttcgaa tgcagtgcgt gctttgaata ttcgggcagt
     2101 tagcgcatta atgttgggca agacccgaaa cgcattggat gatctacagc aagcgctcgc
     2161 tttacttgaa caacataacc tatctgcatc atctcagacc gtacaaagta tgtatcatct
     2221 aggcaccgcg ctcgtctttt cacaagacta tgcacaagca cacgccatgc tagctcgtac
     2281 tgtggagctt tcaaaaaaag gcggcttacg ccgtttggaa gctgccgcat tgaccatgtt
     2341 aggtcagatc gccttgaatt gtggacaata caagcaagct gttgaacttt atgatcgatc
     2401 cattgaagtt gcaggagcct catatctacc cggcatgtgg ggaaaatttg ccgggcgagg
     2461 ttgggcaaat ctgcgcatgg ggaatctgct tgccgcacat gc
//
LOCUS       DAOREF010000466         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30375_length_2501_cov_3.759608, whole genome shotgun sequence.
ACCESSION   DAOREF010000466 DAOREF010000000
VERSION     DAOREF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30375_length_2501_cov_3.759608"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..984)
                     /locus_tag="PKI33_10025"
     CDS             complement(199..984)
                     /locus_tag="PKI33_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37393.1"
                     /translation="MATSDYKARQVRAKTSEVLEYENNDEKPGKPLHILIPGGLIALG
                     TLALIVFIAYLTWFDDSLADSTGTFLCLILSPFYVGGVFLFSYGYELYNIPRAIRLTA
                     IIVFVTLASVVIIAVLFFLLGNMKGGGSSSGSNNNSRSKSSSRSSKPSGSGLGGAVLG
                     GMMGSSSSNSSSSSNSSSGGHYHSSGPVFIGGLGGTRVETREVTKEVIKEVPKEPMPV
                     TCPFCTRSYIPVEHNYVCPSCGAATPKDLIEESQMPDEDTNKK"
     gene            complement(1000..2379)
                     /locus_tag="PKI33_10030"
     CDS             complement(1000..2379)
                     /locus_tag="PKI33_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNM37394.1"
                     /translation="MKKLFPLFIIVSILLSACGAVEQPATSTEEATTGQGTQEAAPSE
                     EPSVEATTKLDVNIDALRGQEVRVWTPWYGVEQSLFESFVNEFNSSNPYGIKVSAESQ
                     SNFTNVYEITTAALPTENKPDLVIALPEHAQGWYQDQVSIDLTDYAEDPVYGLDESDI
                     PYAIWNQDIAGTARVAVPAQRTARVMLWNETWANELKIKSAPVSVDDFRKQACNAHTS
                     MKKDAFAENDAMGGWIVDTEPMTVYSWLLAFHGGVLEGDNYRFLAPNNIDAFKYLREL
                     AEAGCSWQGSSDPITSFAKREALFITVSLSDLPTVARAFVAAENRDTWKVLPFPNGED
                     GVIAVYGSSYVIFQSTEEKQMAAWLFVRWLLEKEQDARWVEATHNFPLHTSTVDLLGD
                     YEKNHAQWRQAVELLPLGELQPQLGSWRTIKLVLGDGFDHIYRVNVSSGQVAAILAQM
                     ESLAKDLNK"
BASE COUNT          619 a          657 c          617 g          608 t
ORIGIN      
        1 catcacacag gattcttggc aggcgttcaa tcccaagagc aaatgtgcgg ctggcagttc
       61 gtagcaaatc ttttgggttc atgggttctt tccttgacaa taaatttatg ttgttaacac
      121 tataccgcat ctattcgata gcattaatag aaaaaaggac gatggaagcc aaaacttcca
      181 tcgtcctttt gggactgatt attttttgtt tgtatcttcg tctggcatct gcgactcttc
      241 gatcaggtct ttcggagtcg ccgcaccaca actcgggcag acgtaattat gctctacagg
      301 aatgtacgag cgagtacaga agggacaagt aaccggcatt ggttctttcg gtacctcttt
      361 gattacttct ttggtgacct cacgagtctc aacacgtgtg ccgcccagcc cgccaatgaa
      421 gacgggtccg cttgaatggt agtgcccgcc cgaagaggaa ttggaagagg aagacgaatt
      481 gctggaggag cttcccatca tgccgccgag caccgcaccg cccaacccac tgcccgatgg
      541 tttggaagac cgagaggacg atttgctacg actgttgttg ttactgccgg aactggaacc
      601 gccgcctttc atgttcccaa gcaaaaagaa aagcacagca ataatgacca ccgaagccaa
      661 cgttacgaat acgatgattg ctgttaaccg aatggcacgc ggaatgttgt aaagctcgta
      721 gccataactg aaaaggaaaa cgccgcctac atagaatggt gagagaatca ggcagaggaa
      781 tgtgccggtt gaatcggcaa ggctgtcgtc gaaccaggtt aagtaggcga taaagacgat
      841 caacgccagc gttcccagtg cgatcaaccc gccgggaatc aagatatgta aaggcttgcc
      901 gggtttctca tcgttatttt catattccag cacctcgctg gtttttgccc gcacctgcct
      961 tgctttatag tccgaagtcg ccatgcctgt atcctttcat cacttattca ggtctttcgc
     1021 taaagattcc atttgcgcaa ggatcgccgc tacctgtccg ctcgaaacgt tgacacgata
     1081 tatgtggtca aacccatcgc ctaacactaa tttaattgta cgccacgagc ccagctgagg
     1141 ttgcaactct ccgagcggca gcagctccac ggcttgacgc cactgggcat gattcttctc
     1201 atagtcgccg agcaggtcca cggtcgaggt gtgaagcgga aagttgtggg tcgcctctac
     1261 ccaacgagca tcctgttctt tttccaagag ccaacgcacg aacagccaag ccgccatctg
     1321 cttttcttcg gttgattgaa agatgacata cgaagaacca tacacggcaa tgactccatc
     1381 ttctccattg gggaagggca gcactttcca cgtatcgcga ttttcagcgg cgacgaaagc
     1441 ccgcgcaacg gtaggcaaat cgctcaggct gacggtgatg aacaacgcct cacgcttcgc
     1501 gaacgaagta atcgggtcgc tggacccttg ccaggaacaa cccgcttctg ccagctctcg
     1561 caaatatttg aaggcatcaa tattgttcgg agcgagaaaa cgataattgt ctccctccaa
     1621 cacgccgcca tggaacgcca acaaccacga atagacggtc atcggttcgg tatcgacgat
     1681 ccaaccaccc atggcatcat tttcggcgaa cgcatccttc ttcatcgaag tatgcgcatt
     1741 acaagcctgc ttacgaaaat catccacaga gactggtgca gatttgatct tcaattcatt
     1801 tgcccaagtc tcgttccata acatcacgcg tgctgtgcgt tgtgccggaa ccgccacccg
     1861 cgccgtgcct gcgatatctt gattccaaat tgcatatgga atatcgctct catccaatcc
     1921 atagaccggg tcttcggcgt aatcggttag gtctatgctc acctgatctt gataccaacc
     1981 ctgggcatgt tcaggcaatg caatcacaag gtctggtttg ttttcggtgg gcaaagcggc
     2041 tgtggtgatt tcgtatacgt tcgtaaagtt gctctgactc tccgccgaga ccttgatgcc
     2101 atacgggttg ctggaattaa actcgttgac aaagctctcg aacagacttt gctccacgcc
     2161 ataccacggc gtccacacgc ggacttcctg cccgcgcaac gcatcgatat tgacatcaag
     2221 cttggtcgtt gcttcgacgc tgggttcttc gctgggagcg gcttcttgcg ttccctgccc
     2281 ggtggtggct tcttctgtag aagtggcagg ttgctctacc gcaccacaag cggacaatag
     2341 aatggatacg atgatgaaga ggggaaatag ttttttcatg gaatcaagga gccaataaca
     2401 taaaggggac gggagtgaac actaaaatga agaccacgat cattagatag gctagggctt
     2461 tacgggcggg gtctaactgg gtgatctggt ccataggttg c
//
LOCUS       DAOREF010000467         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30453_length_2498_cov_4.011461, whole genome shotgun sequence.
ACCESSION   DAOREF010000467 DAOREF010000000
VERSION     DAOREF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30453_length_2498_cov_4.011461"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2263
                     /locus_tag="PKI33_10035"
     CDS             <1..2263
                     /locus_tag="PKI33_10035"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37395.1"
                     /translation="LLTDILRECPAVKLLVTSRELINLPAEWLVEVQGLPYPPTFEKN
                     ADMQYPSVELFIHQARRVNPNFSLEEQEYAVNRICQLVAGFPLGIELAANWVRLLSCE
                     EIVERLERGGEILTTDLPELNPSLRSVLDSTWAMLTDEEQTVFRRLSVFRKGFTLSAA
                     QQVADANLPVLNSLMNKSMIRRDENGRYVLHELLVQFGSYHLKLAPEQERETRQKHAR
                     FYSSFLQSRRAAVQDAYDKAVLNGIDAEIENIRAALEWHFELRDVEAIAAYLETLLQF
                     NRRKGWFRESVIILEKACAMENVPAMQMGYWYRWLGEAHYNLGNIAASTQNLETALSY
                     FGYPMPKHPLRLALASYWQAIVQIWRRIWKPGLHVVEYEELEKRLATVAVCERLSQIY
                     WFQSKSAAIFYLTFFPLNLMERVENAPALTQVNAAIGLSLSPIPNAAKYYLQNGLQAA
                     QKQNDPLAVGQALEHAGLYYSGLGEWEQAEASLQKSWKILEAVEFHRQWEEAAGLLSE
                     MKSLRGDFAGAIQIQQKIIASAHQRGDTQLRFHDLTGLALNNLRLGDIETANKHLHNA
                     AELLGRNYVFVNNIRFYGVSVQVALQQGDFHTAKEFAQLAMSLISKVSPTSFYILEGY
                     AGVSEVYLTAFENGKNSEDLKFAQRAVKALLEFGQVLKVGQPRAWLHQGLLYWVEGQQ
                     KQAFRVWEKSLALAEQLSLPYEAARAHYEIGRHLSLNDAARQIHLGKAIEIFSGLGAK
                     RELMQAQNASHNK"
     gene            complement(2302..>2498)
                     /locus_tag="PKI33_10040"
     CDS             complement(2302..>2498)
                     /locus_tag="PKI33_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNM37396.1"
                     /translation="NAPYTVAIVKLDEGPMVTAQLTDVDNSEVQIGMPVEMVTRKMRN
                     DGDERGLIVYGYKFRPAVTA"
BASE COUNT          605 a          600 c          704 g          589 t
ORIGIN      
        1 tctgttgacc gatattttga gggaatgtcc cgcggtgaag ttgctggtca catcccgcga
       61 actgatcaac ctgccagcgg aatggctggt ggaagtgcaa gggctgccgt acccgccgac
      121 attcgagaag aacgccgata tgcaatatcc atcggtcgag ttgttcatcc atcaagcccg
      181 ccgtgtgaac ccgaactttt cactggaaga gcaggagtat gccgttaatc gtatttgtca
      241 gttagtggcg ggctttccgc ttggcatcga acttgctgcc aattgggtca ggctgctttc
      301 ctgcgaagag atcgtcgaac ggttggaacg cggcggcgaa attctcacga ccgacctgcc
      361 cgagctaaat ccgtccctgc gctcagtgct ggattcgaca tgggcgatgc tcactgatga
      421 agaacagact gtctttcggc ggctgtctgt ttttaggaaa ggctttacgt tgtctgccgc
      481 acaacaggtg gcagatgcca acctgcccgt attgaacagc ctgatgaata agagcatgat
      541 acggcgcgat gagaacgggc gctacgtact gcatgagttg ctggtgcagt tcggttcgta
      601 tcatctgaaa ctggctcccg aacaggaacg agaaacacgc caaaagcatg ctcggttcta
      661 cagcagtttt ttacaatcgc gccgcgccgc tgtgcaggat gcctatgaca aggctgtgtt
      721 gaacggcatc gatgccgaaa tcgaaaacat ccgcgctgcg ctggagtggc attttgagct
      781 gagggatgta gaggcaatcg cagcttattt agaaaccctg ctgcagttta acaggcgcaa
      841 aggctggttc cgcgagtcgg tgattatcct ggaaaaagcc tgcgcgatgg aaaatgtccc
      901 tgcaatgcaa atggggtatt ggtatcgctg gctgggtgaa gctcattaca accttgggaa
      961 tatcgcagcc agcacacaga atcttgaaac tgctctgtct tattttgggt acccaatgcc
     1021 taaacacccg ctgaggttgg cgcttgccag ttattggcag gcaatcgtgc aaatctggcg
     1081 gcggatctgg aagcctggct tgcacgtggt cgaatatgaa gagctggaaa aacgactggc
     1141 tactgttgct gtatgcgaac gattgagcca gatctactgg tttcaaagca aaagcgctgc
     1201 tattttttac ctgacgtttt tcccgctcaa tctaatggaa cgcgtagaga atgcgcctgc
     1261 actgacacag gtcaatgccg ccattgggtt aagcctcagt cccatcccca atgcggcaaa
     1321 gtattacttg caaaacggat tgcaagcggc acagaaacaa aacgatccac tcgcggtggg
     1381 gcaggctttg gagcatgcgg ggttgtatta ctcagggctc ggggaatggg aacaggcaga
     1441 ggcgtcgtta caaaaatcct ggaaaattct cgaagcggtg gaattccaca ggcagtggga
     1501 agaagctgca gggttgcttt ctgagatgaa atctctgcgc ggcgattttg caggcgcgat
     1561 ccagatccag cagaagatca tcgcttcggc tcaccagcgc ggcgatacgc aactccgctt
     1621 ccacgatttg acagggttgg cgcttaacaa cttgcggctg ggggatatcg aaacagcaaa
     1681 taaacatttg cacaacgctg cagaattatt aggcaggaac tacgtgttcg tcaacaatat
     1741 tcgtttttac ggagtatccg tgcaggtggc tttacagcag ggcgactttc acaccgcaaa
     1801 agaatttgcc cagctggcta tgtcgctgat ttcaaaggtc agcccgacat ctttctatat
     1861 attggaaggc tatgcaggcg tttcggaagt gtatctgacc gcgtttgaaa atgggaaaaa
     1921 ttccgaagac ctgaagttcg cccagcgagc ggtgaaggca ttgcttgagt tcggtcaggt
     1981 gctgaaagtg ggtcagccgc gggcatggct gcatcaaggt ctgctctatt gggtggaggg
     2041 gcagcagaaa caagcatttc gggtttggga aaaaagcctg gcgctggcag agcagttgtc
     2101 cctgccgtat gaggcggcgc gggcgcacta tgagatcggg cggcacttgt ccctcaacga
     2161 tgctgcgcgt caaatccatt tgggaaaagc gattgagatc ttctcaggct tgggggcaaa
     2221 gcgtgaatta atgcaggcgc agaacgcatc tcacaataaa taaaaaagcg gcggagaaaa
     2281 tctccgccgc ttttgtgtca tttatgctgt tactgctggt ctaaacttat atccataaac
     2341 gatcagccca cgctcatcac catcatttcg catcttgcgg gtgaccattt ccacgggcat
     2401 gccgatctgg acttcggaat tgtccacgtc ggtcaattga gccgtcacca tgggaccttc
     2461 atctagtttt acgattgcca cggtgtaagg ggcatttg
//
LOCUS       DAOREF010000468         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30475_length_2497_cov_3.364455, whole genome shotgun sequence.
ACCESSION   DAOREF010000468 DAOREF010000000
VERSION     DAOREF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30475_length_2497_cov_3.364455"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1539)
                     /locus_tag="PKI33_10045"
     CDS             complement(121..1539)
                     /locus_tag="PKI33_10045"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNM37397.1"
                     /translation="MSKTLVMKFGGTSVGSVDALKNAVQIIRDAKKDWERVVVVTSAM
                     SGVTNLLIDSAASASHGNIDSLSQSASMLREKHFVAADALVPGKARCDQIKAEIDDLI
                     FLLVDLCKAIAVLGEASPRAMDTVASLGERMSVRLLAAVVESAGIPAQAVESTEFIVT
                     NAHFQNAHPDFNITTEKTRGKLNPIMDKGIVPITTGFIGATPEGAITTLGRGGSDYSA
                     AIIGAVLPADDVWIWTDVDGVMTTDPRIVPQAVTLPEISYNEIAELAYYGAKVLHPKT
                     IRPVLDAGIGLRICNTFNPSHPGTRLIASVKSNGKVEGKVIKAVTAIRKQRLITIEGR
                     GMLGVPGVAARAFGAVASTGTSVPLITQASSEQSICFAVPSELAPTVLSSLEKSFARE
                     IEDEDIDRVWATEDVSIITVVGAGMRHTPGVSGRVFSKLGNDGVNVLAIAQGSSEVSI
                     SLVVDAVDTENAVKALHELIVQ"
     gene            complement(1619..1915)
                     /locus_tag="PKI33_10050"
     CDS             complement(1619..1915)
                     /locus_tag="PKI33_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37398.1"
                     /translation="MFLFHSANVMKAQTSENGVPEAGLRLDSKQETERLYILGRILHD
                     MQNASIFDADPISPLTQFKPSHRIPPNMNSAHFSMTMTTTTSGTHREAYVLGMK"
     gene            1991..>2497
                     /locus_tag="PKI33_10055"
     CDS             1991..>2497
                     /locus_tag="PKI33_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HNM37399.1"
                     /translation="MSTIRIGFLFTRLRAEEKYLLEELEKRPNVDIVRINDGDHFFDI
                     SSAPEKVDVLFERSVSYSRGLYISKIYEANGVTVVNTPQVAERCGDKYVTSQLLVQNG
                     IPTPRVLMAFDEESALAAVEAVGYPCVLKPVVGSWGRLLAKVDNRHMAESLIEHKATL
                     GVHHQGFYI"
BASE COUNT          551 a          650 c          688 g          608 t
ORIGIN      
        1 actctagcag aaacctcatg gaccttcaag tcagggtagc aacttgagtt aagatattgt
       61 tttgtcaaaa gtcgaaggtc aaaggtcgca ggactttcga cttttgactt tcgacagatt
      121 ctattggacg atcaactcat gcagcgcctt aaccgcattt tccgtatcca ctgcatccac
      181 taccagtgag atggacactt ccgaagagcc ctgcgcaatc gccaggacat tcacgccgtc
      241 gttgccgagt ttgctgaata cgcgacctga aacgcccggc gtgtgacgca ttcctgcacc
      301 gacgacggtg atgatggaca catcttcggt cgcccagacg cgatcaatgt cttcgtcttc
      361 gatctcgcgg gcaaaagatt tttcaagcga agacaaaacg gtgggggcga gttcggaggg
      421 cacggcaaaa cagatcgact gttccgatga ggcctgcgtg atgagaggta cgcttgtacc
      481 cgtagatgcc actgcgccga atgcgcgcgc cgccacaccc ggtacgccta gcattcctcg
      541 cccttcgatg gtgataagcc tctgcttgcg gatagccgta accgccttga tgacttttcc
      601 ctccaccttg ccattggatt tgacgctggc aatcagccgc gtgccgggat ggctggggtt
      661 aaatgtattg caaatacgca agccaatgcc tgcatccaag acggggcgga tggtcttggg
      721 gtgcagaact tttgcgccgt agtaggccag ttcggcaatt tcgttgtaac tgatttctgg
      781 cagcgtcacc gcttgcggca caatgcgcgg atccgtggtc atcacgccgt ccacgtctgt
      841 ccagatccag acatcatctg caggaagcac cgcaccgata atggcggcgg aataatccga
      901 cccgccgcgg ccgagggtgg tgatggcgcc ttcgggggtt gcgccgataa aaccagttgt
      961 aatagggaca atgcctttat ccatgatcgg attaagtttc ccccgcgtct tttcggttgt
     1021 gatattgaaa tcgggatgag cattttgaaa atgcgcattg gtgacgatga attcggtcga
     1081 ctcaacggct tgtgcgggga tgcctgcgct ttccaccaca gcggcgagca gacgcacgct
     1141 catccgctca ccgagcgagg cgaccgtatc cattgcgcgt gggctggctt cgccgagaac
     1201 tgctatggct ttgcacaggt ccacaagcag gaagatcaga tcgtcgatct cggctttgat
     1261 ttggtcacac cgggccttgc ctggaacgag cgcgtcggcc gcgacgaagt gtttttccct
     1321 cagcatggac gcggactggg ataacgaatc gatgttcccg tgcgaagccg aagcggcaga
     1381 gtctattagc aggttcgtca cgccagacat ggcggatgtt acgacgacga ctcgctccca
     1441 atcctttttc gcatcgcgga tgatttgaac ggcattcttc aacgcatcga cagacccgac
     1501 ggatgttcct ccgaatttca tgaccagcgt ttttgacatt gtattctcct tcggtaaggt
     1561 aacaaaaacg ccccgcattt ctgcgaggcg ctgtgtgttg tgattaacct tcgttcggtt
     1621 acttcatccc aagcacatac gcctcgcggt gggtaccgga ggtggtagtg gtcatggtca
     1681 tggagaagtg agccgagttc atgttgggcg gtattctatg cgagggcttg aattgagtca
     1741 agggggaaat tggatccgcg tcaaatattg acgcattttg catatcgtgt aaaattcgcc
     1801 ccaatatgta cagcctttct gtttcctgct tcgagtccaa gcgcaagccc gcctccggaa
     1861 ccccgttctc ggatgtttgg gctttcatga cattagcaga gtgaaataga aacatatctc
     1921 acgtccttgt caccccaaca cgctctccga aaacggaggg cgtgttttta ttttgcctaa
     1981 ggagaaaata atgagcacca ttcgaattgg ttttttgttt acccgcctgc gagcggagga
     2041 aaagtatttg ttggaggaat tggaaaagcg tcccaatgtg gatatcgtcc gcatcaatga
     2101 cggcgaccac ttctttgata tttccagcgc ccccgaaaaa gtggatgtgc tcttcgagcg
     2161 ttcggtctcg tactcgcgcg gactctacat ctcaaagatt tacgaagcca acggcgtgac
     2221 cgtggttaat acgccgcaag tggcggaacg ctgcggtgat aagtacgtca ccagccagtt
     2281 gcttgtgcag aacggaattc caactccccg agtgctgatg gcttttgacg aagaatccgc
     2341 tctggcggcg gtggaggcgg ttgggtatcc ctgcgtgttg aaacccgttg tcggttcgtg
     2401 gggacgtctg ctcgccaaag tggataaccg tcatatggcg gagtcgctaa ttgagcataa
     2461 agccacattg ggagtccatc atcagggttt ctatatt
//
LOCUS       DAOREF010000469         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30519_length_2495_cov_4.217213, whole genome shotgun sequence.
ACCESSION   DAOREF010000469 DAOREF010000000
VERSION     DAOREF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30519_length_2495_cov_4.217213"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PKI33_10060"
     CDS             complement(<1..1410)
                     /locus_tag="PKI33_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNM37400.1"
                     /translation="MQNHKKTFLSFLAIIFFLSACSSNQATEVPTNTEQVFLTLPVTE
                     TPAPPTETLLPTNTPLPTLERAKYTLNTTIDYDAHTVTVDETILYPNLTGNQLNALVL
                     AVIPNLWDGSFSLTGISIDGQPITTYTLNGQRLDVSLASFLPAGGTAEIKLQYTLTLP
                     FAEQEDPSVSRPRIYGYTSRQVNLVNWYPFVVPYINGEWTLHDPWFYGEHLVYDSADY
                     EVNLSFVPSGLTPIVAASGVPEAQPDGSTRYTISAARTFALAASRDFQTASTQVGDVT
                     LTSYYFPFSEAGGQAALQASAQALQVYGARYGAYTHKSLAIVMGDFNDGMEYSAFYYL
                     SKDFYNLYDGTPANYLTFVAVHETAHQWWFEQVGNDQALQPWLDESLATYSERVYYEN
                     TYPDLVSWWWSYRIDFYKPEGFVDIPIYEGQGFRPYTNATYFQGAHFLEKVRERIGDE
                     AFFAFVQDYLNQGRGKIVTA"
     gene            complement(1509..1946)
                     /locus_tag="PKI33_10065"
     CDS             complement(1509..1946)
                     /locus_tag="PKI33_10065"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNM37401.1"
                     /translation="MALIATSIKINKPASIIFAHLTNVENMKNEYVEKIEIDGALRLG
                     AKYKMFTKAGGRLIETVNEVVGFEQDKLVSTKTFAAPPASDMVSTYILEDEGGATKVT
                     FQSEAQLTVPGMPSMPGMEDMMKKQMIAGFDATLAALKKKLEG"
     gene            complement(2119..>2495)
                     /locus_tag="PKI33_10070"
     CDS             complement(2119..>2495)
                     /locus_tag="PKI33_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNM37402.1"
                     /translation="TVAILYKPFLVISFDPVLAATLRLPAELLRNLMLVLLALTVVVS
                     LQTVGVSLAAAMLVTPAATAYLLTRRLLPMMFVSAAIGALSSVIGLYLSYYFNIVSGS
                     AIVLTATLFFLLGFIWKRLRKA"
BASE COUNT          619 a          503 c          757 g          616 t
ORIGIN      
        1 ggctgtgacg atcttgccac gaccttggtt gagataatct tgcacaaagg cgaagaaggc
       61 ttcatcacca atccgctcgc ggaccttctc gaggaagtgt gcgccttgga agtaagtggc
      121 attggtatat gggcggaagc cctgaccttc ataaataggg atatccacaa aaccttcggg
      181 cttgtaaaag tcgatacggt aagaccacca ccatgaaacg aggtcaggat aggtgttttc
      241 gtaatagacc cgttctgaat acgtggcgag tgattcgtcc aaccagggtt gcagggcttg
      301 gtcattgccc acttgttcaa accaccattg atgcgcagtt tcgtgaacag caacgaaggt
      361 caaatagttt gctggagtgc catcgtataa attgtagaaa tctttgctga ggtagtagaa
      421 agcggaatat tccatgccgt cattgaaatc gcccatgacg atagcaagac ttttgtgagt
      481 ataagcgcca taacgtgcac catacacttg caacgcctgt gcagaagctt gcaaggcagc
      541 ttgaccacca gcttcactga aagggaagta ataactagtg agagttacgt cgcccacttg
      601 ggtgctggcg gtttgaaagt ctcggctggc ggcgagggcg aaggtgcgtg cggcgctgat
      661 ggtgtaacgg gtgctgccat cgggctgcgc ttcgggcact ccgctcgcgg cgacgatggg
      721 tgtaagccca gaaggtacaa agctgaggtt gacttcgtaa tcggcggagt cgtaaacgag
      781 atgttcgccg tagaaccatg ggtcgtgtaa ggtccattcg ccgttgatgt agggcaccac
      841 aaagggatac cagttgacga ggttgacttg tctgctggtg tagccgtaaa tgcgcggacg
      901 ggacacgctt gggtcttctt gttcggcgaa gggaagtgtc agcgtgtact gaagtttgat
      961 ttcggcggtt ccgcctgcag ggaggaaaga ggcgagcgac acgtcgaggc gctgtccgtt
     1021 gagggtatag gtggtgatgg gttgcccgtc gatggagatg ccagtgaggg agaagcttcc
     1081 atcccaaaga tttggaatga cagccagaac gagcgcgttg agttggttgc cggtcaggtt
     1141 cgggtacaga atggtttcat ccacggtgac ggtatgcgcg tcatagtcga tggtggtgtt
     1201 gagggtgtac ttggcgcgtt caagggtggg taggggcgtg ttcgtcggca gcagagtctc
     1261 ggttggtgga gcaggtgttt cggttacagg caaagtgagg aaaacctgtt ctgtgttggt
     1321 gggaacttct gttgcctgat ttgaagaaca tgcggaaagg aaaaagatga tggcaaggaa
     1381 cgagaggaaa gtctttttgt gattttgcat gggtgcaatt ctaccgcttg tcaattaatt
     1441 ttaaataatc tggaaataaa aacatcctgt tgaagccaac aggatgtttg gtcgttcatc
     1501 gagcgagatt aaccttctaa ttttttcttc aatgcagcga gggtggcgtc gaaaccggcg
     1561 atcatttgtt tcttcatcat atcttccata ccgggcatgg agggcatacc ggggacggta
     1621 agttgggctt cgctctggaa ggtgaccttg gttgcgccgc cttcatcttc gaggatgtag
     1681 gtgctgacca tatctgaagc gggaggggcg gcaaaggttt tggtgctaac caatttatct
     1741 tgctcaaagc cgaccacttc gttgacggtt tcgatcaagc gtccgccagc tttggtgaac
     1801 atcttgtact tggcgccaag acgaagagcg ccgtcaattt cgatcttttc cacgtattcg
     1861 ttcttcatgt tttcgacatt ggtcaggtga gcgaagataa tgctcgcggg tttgttgatc
     1921 ttgatggaag ttgcaataag tgccatgatt tttctccttg ggaaatattt gtaaacgatt
     1981 gaatgtgatt tttattatat ggatggcgac agtttctaca atgaacttaa gggggatttg
     2041 gaggatgacc attagtacta taaaaaaagc cgcctgtgga tcaaatccat ggacggcttt
     2101 gcattgttta ttgctgtttt atgcttttcg caatcgcttc cagataaaac ctagcaggaa
     2161 gaacagggtg gcggtcagca caatggcgga accagagacg atattgaagt aatacgaaag
     2221 gtacaagccg atgacagaag ataaagctcc aattgcggca gagacgaaca tcatgggcaa
     2281 tagacggcgg gtgagcaggt aggcggtcgc agctggggtg acgagcatgg cggcagcgag
     2341 actaacaccc acggtttgca aggagacgac aacggtgaga gccaacaaga caagcatcag
     2401 gttgcgaagg agttcggctg gcaggcgcaa ggtcgctgcg aggacggggt caaaggaaat
     2461 gacaagaaag ggtttataga gaattgccac tgtta
//
LOCUS       DAOREF010000470         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30580_length_2493_cov_2.802707, whole genome shotgun sequence.
ACCESSION   DAOREF010000470 DAOREF010000000
VERSION     DAOREF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30580_length_2493_cov_2.802707"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKI33_10075"
     CDS             <1..424
                     /locus_tag="PKI33_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37403.1"
                     /translation="RLFGGGSARLALTDPENLVETGIEIEARLPGRREQGLALLSGGE
                     RSLTAIALVFALLKVSPTPVCVMDEVDAMLDEANVGRFRDLLVDLSKDTQFIVITHNR
                     NTVQAADVIYGVTMGRDSASQVISLRLDQVTDDMLKRG"
     gene            580..1005
                     /locus_tag="PKI33_10080"
     CDS             580..1005
                     /locus_tag="PKI33_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37404.1"
                     /translation="MVNKKRKPVIQSLLVEYQAAQDSAHHHDNLVWTTTGIIWGAELV
                     LIGFVVQSADDPGLKLPVASVCLLAVFKLIYLWIMTLQFNSVKNQKYVRCKEIESLLS
                     LEQHSRLHYPKKWGRFFYSLAMILFIVTWVAILVTSVLS"
     gene            1080..1742
                     /locus_tag="PKI33_10085"
     CDS             1080..1742
                     /locus_tag="PKI33_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37405.1"
                     /translation="MSKSQKDQSRPKSEWKRWIIVFFCLALIVVRYFYPAVQIDAPTV
                     WLVVIAAIAFLLPELKSFTPYIKRVKIGDTEVELKDEIAKLGNEVEKAKQSPPPGGKK
                     TARKKVTKKPEHTFDATIEKALNDPRATLLILSAKIEEELKKRLVEAGVDIDRVYSLK
                     NLSRLAVDNKLLSIESASALNDFSVVRNKVAHGEAFDVEDHVIYSLISIGVDILKLIM
                     AR"
     gene            1791..>2493
                     /locus_tag="PKI33_10090"
     CDS             1791..>2493
                     /locus_tag="PKI33_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37406.1"
                     /translation="MITTHRAFLNDQDKQLMIDLAGQFQSDHLHVVDLPYRLSSWGFD
                     DKENVRLWFDGENLVAWAVLQSPFWAIDYACHADYPALFKEILAWADGRAQAVKGTPY
                     ERSAWFVNVFSGQEARKKDLEAMGFACQSDVGEDSWSKVFMKLSPEPKLYEPPVGFTV
                     RPLGGDVEKYVALHQSVFESKNMTIEWRGRTLGSPLYKPELDLVVESPTGELAAFCIC
                     WFNEKLSAGQVEPLGV"
BASE COUNT          654 a          455 c          675 g          709 t
ORIGIN      
        1 gcgtctgttc ggcggcggct cggcgcgact ggcgttgacc gaccctgaga atttggttga
       61 gaccggtatc gagatcgaag cccgtctgcc ggggcgacgt gagcaaggct tggcgttgct
      121 ctccggcggt gaacgaagtc tgaccgcgat tgccttggtg tttgcgctgc tcaaagtgtc
      181 gccaactccc gtctgcgtga tggacgaagt ggacgcgatg ctcgacgaag ccaacgtggg
      241 acgcttccgt gacctgctgg tggacttgag caaggacacg cagttcatcg tcattacgca
      301 caaccgcaac acggtgcaag ccgcagatgt gatctacggt gtgacgatgg gacgcgactc
      361 ggcgagtcag gtgatcagtt tgagactcga tcaggtgacg gatgatatgt tgaaacgcgg
      421 gtaggggtgt ggaaagtaga aagtggtaag ccctgtccca gtttctaggg cggggctttt
      481 ttgttgaggg gagaggatat aatgaagaac atgataaata tagtattttt ataaagaaat
      541 gtagttatta ggtgttagta tttaggatgg tggtgattta tggtcaataa aaaacgtaaa
      601 ccagtcatac aatccttact tgttgaatat caagcagctc aagattctgc acaccatcac
      661 gataacctag tgtggacaac gactgggata atttggggcg ctgaattagt tttaatagga
      721 tttgttgttc aaagtgcaga tgacccagga ttaaaactac cagttgcttc agtttgttta
      781 ttagctgttt ttaagttgat atatctttgg ataatgactc ttcaatttaa ttcagtaaaa
      841 aatcaaaagt acgtacggtg caaagaaata gaaagtctac taagccttga acaacactca
      901 agattacact acccaaaaaa gtggggtaga tttttttaca gtttagccat gatacttttt
      961 attgttacat gggttgcgat acttgtaaca tcagtattgt cttgaaaatg atattttaaa
     1021 gagcacattc ttgcagattt tcgatagtgc ttttagttgt tggcgttaag aggtgcaaaa
     1081 tgagcaaatc acaaaaagat caatcaagac ctaagtcgga atggaagcgg tggattattg
     1141 tatttttctg cttggctctc attgtggttc gatattttta tccagctgtt caaattgatg
     1201 ctccgacggt ttggctagtc gttattgctg ctattgcatt cttacttcct gagttgaaat
     1261 cttttacacc atatataaag cgagtgaaaa ttggagacac tgaagttgaa ttgaaggatg
     1321 agatagcaaa gcttggtaat gaggttgaaa aagccaaaca atctcctcct ccaggcggga
     1381 aaaagaccgc aaggaaaaag gtcactaaaa aacctgaaca tacttttgat gcaacgattg
     1441 aaaaggcgct taatgatcct cgcgctactc ttctaatttt gtctgcaaaa attgaggagg
     1501 aactcaaaaa gagacttgtt gaggcaggtg ttgatattga tagggtatat tctctaaaaa
     1561 acttatctcg tcttgcagta gataataaat tgctttcaat tgagtcagct tcagctttga
     1621 atgatttttc tgtagtgcga aataaagttg cgcatggcga agcttttgat gttgaggatc
     1681 atgtaattta ctctctcatt tcaattggtg tagatatttt gaagcttatt atggcgcgtt
     1741 gattttttat gccgcaggga tagggtattt caataaaaag gaaatcttta atgataacaa
     1801 ctcaccgtgc ttttctaaat gaccaagata agcaattgat gatcgacctg gctggtcaat
     1861 tccagtcaga ccacctacac gtagtagacc tgccgtatcg gctgagttcg tggggattcg
     1921 acgataagga aaacgtccgc ttgtggtttg atggggagaa tctggtcgct tgggcggtgc
     1981 tgcaaagtcc gttttgggcg attgattatg cttgccatgc ggactatcca gccttgttca
     2041 aggaaatcct tgcatgggcg gatggacgcg ctcaagctgt gaagggtacg ccgtatgagc
     2101 gttcggcttg gtttgtcaac gtgttctcgg ggcaagaagc tcgcaaaaaa gatttggaag
     2161 caatgggctt tgcctgtcaa tcggatgtgg gcgaggactc gtggtcaaag gtgttcatga
     2221 agctttcgcc tgagccgaag ctatacgagc cgcctgtcgg gtttactgtc cgcccgctgg
     2281 gtggggatgt ggagaagtat gtggcgttgc atcagtctgt gtttgagagc aagaatatga
     2341 cgattgagtg gcgcgggcgt acgctgggaa gtccgctgta taagccggag ctagatctcg
     2401 tggttgagtc accgacgggg gagttggcgg cgttttgcat ttgctggttc aatgagaaac
     2461 tgtccgcggg gcaggtcgag ccgttgggtg tgc
//
LOCUS       DAOREF010000471         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30629_length_2490_cov_4.829158, whole genome shotgun sequence.
ACCESSION   DAOREF010000471 DAOREF010000000
VERSION     DAOREF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30629_length_2490_cov_4.829158"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKI33_10095"
     CDS             complement(<1..661)
                     /locus_tag="PKI33_10095"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM37407.1"
                     /translation="MPFVQGIHYSFQQSKETNPSRPPLIFIHGAGGSFLSFHPYLRRM
                     QGETIYALDLPGHGKSVGKGRQSIEEYAEDIARFMDDLQIASAVMVGLSMGSGIALSF
                     ALKYPHKVSALVVMGGGAKLRVAQSTLENVGKPETFEATVETINRACFSTYASNELVE
                     LSKKSMLSTGPDILFSDYLACNHFDVTTQLNQIRIPTLLLCGMEDAMTPPKYSQYLRD
                     RL"
     gene            complement(665..2422)
                     /locus_tag="PKI33_10100"
     CDS             complement(665..2422)
                     /locus_tag="PKI33_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNM37408.1"
                     /translation="MKRFETPYMADWFAISLRWIMLVGLIVSLGLGQRLDASASWPLG
                     LMIVWNLGMTALAGLNIRTQYHRRLNIAMDLILTAAFFWAQGGVRGPAFWAGLLPILT
                     GSIYFELIGAVVAAILFSGFVIYTGTRVDGNITLAFLVAAILSLLGVLFGFLGRRLTD
                     HMRKNRALWVDAEEKKRAIQNERMRAIYELTSTLSTTLSYKRVLDSALDMSASALNPN
                     LDPEQTTSDPLVGAVMLFNAGKLRIGSARRFTNADMRITFDGAEGVLKKALDEGEPVI
                     FKDVGYDQELGRVVALRNCTGGYVFPLRSGFNVYGVLLFAHPEPDFFNTERTNLLDII
                     GRQAVIAIQNARLYQDLVEEKERLADVYEEARKKLARDLHDGPTQSVAAMAMRLNIAR
                     RMLTKDTKMANEELVKLEELAHRTTKEIRHMLFTLRPLILESQGLGAAIQSMADKMME
                     TFSQRVVVEIDDRAVQQLEMGKQGVIFYIIEEAVNNARKHAAAEAITVRLRQVDTGIV
                     LLEIQDNGAGFDVKAITQNYDKRSSSSLGMINLQERAELVNGLLNIDSAPGKGTNVQV
                     YIPLTEEAADRLHHARRSA"
BASE COUNT          683 a          614 c          567 g          626 t
ORIGIN      
        1 gaagacggtc tcttaagtat tgtgaatatt ttggaggggt catggcatct tccataccgc
       61 aaagaagcaa tgttgggatt ctgatttggt tcaactgggt agtgacatca aaatgattac
      121 aagcgagata atcgctaaat aaaatatctg gaccagtgct cagcatactc ttttttgaaa
      181 gttcaacaag ttcattagaa gcatatgtac taaaacacgc acggttaatg gtttcaacag
      241 tggcttcaaa ggtttcaggc ttacccacat tctcaagcgt ggattgtgca actcgtaatt
      301 ttgctccgcc acccatcaca accaatgcac ttactttatg cggatatttc agtgcaaaag
      361 ataaagcaat gccactcccc attgaaagcc caaccatgac tgctgatgcg atctgaaggt
      421 catccataaa acgcgcaata tcttcagcat actcttcaat agactgtcta cctttgccca
      481 cagacttgcc atgccctggc aggtctaatg catagatggt ctctccttgc atacgccgta
      541 gatatgggtg aaaggataaa aaactgccgc ccgcaccgtg tataaaaatc aacggtgggc
      601 ggctgggatt tgtttctttg gactgttgga acgagtagtg aatgccttgc acgaacggca
      661 tggcttatgc agacctgcga gcatggtgta agcgatcggc agcttcttcg gtaagcggaa
      721 tatagacctg cacgttggtg cccttgcccg gcgcggagtc tatgtttagc aagccgttca
      781 ccaattcagc acgctcttgc aggttgatca tacccaagct gctgctggaa cgcttgtcat
      841 aattttgcgt aatggctttc acgtcaaagc ctgcgccgtt gtcttgaatt tctaatagca
      901 caatccctgt atccacttgg cgcaagcgga cggtaatcgc ttcggcagca gcatgtttgc
      961 gagcattgtt aacagcctct tcgatgatat agaaaatgac gccttgtttg cccatctcca
     1021 attgctgaac agcacggtca tcgatctcaa ccaccacacg ttgtgagaaa gtctccatca
     1081 ttttatctgc catggattga atggcagccc ccaaaccttg cgactcaaga atcaacgggc
     1141 gcagggtaaa gagcatatgg cgaatttctt tggtggttcg atgtgcaagt tcttccaatt
     1201 tgacgagttc ttcgttcgcc attttggtgt ctttcgttaa catacggcga gcgatgttca
     1261 aacgcattgc catggcagct acagactggg taggtccgtc gtgcaggtct cttgctagct
     1321 ttttacgagc ctcttcgtac acatccgcga ggcgctcttt ttcttccacc aggtcctgat
     1381 aaaggcgggc attctgaatg gcgatcactg cctgacgccc aatgatatcc aaaagattag
     1441 tccgttcggt attaaaaaaa tcaggttcag ggtgagcaaa caaaagcaca ccatatacgt
     1501 tgaaaccgct tcgtaaaggg aaaacatatc cgcccgtaca attgcgaagt gcaacgacac
     1561 gtccaagttc ttgatcgtat cctacatctt taaaaataac aggttcccct tcatcaagtg
     1621 cttttttcaa aacaccttct gccccatcaa aggtaatacg catatcagcg ttggtgaaac
     1681 gacgagcgga gccaatgcgt aatttacccg cattaaaaag cataacagcg cccactaatg
     1741 ggtcgctggt cgtttgttct gggtcaagat tgggattcaa tgcagaagcg ctcatatcga
     1801 gcgccgagtc aagcacacgt ttatagctaa gggtcgtgga aagcgtggaa gttaattcgt
     1861 agatcgcacg catacgttca ttttgaatgg cacgtttctt ttcttcggca tcaacccaca
     1921 aagctcgatt cttgcgcata tggtcagtta gccggcgacc tagaaaacca aagagtactc
     1981 ctaaaaggct cagaatcgca gctacgagaa atgcaagggt aatattccca tccacccggg
     2041 tgccggtgta gataacaaag cctgagaaca aaatggcagc caccactgcg ccaattagct
     2101 caaagtagat ggaaccggtc aaaataggga gcaatcctgc ccaaaaagca ggtccccgaa
     2161 cgccaccttg cgcccagaag aatgccgctg taaggatcaa atccatcgca atgttgaggc
     2221 ggcgatgata ttgtgtacgg atgtttaagc cagctagagc cgtcatcccc aagttccaaa
     2281 caatcatgag ccccagaggc cagcttgcac tcgcatctaa tctctgtcca agcccaagag
     2341 aaacgataag acctactagc atgatccagc gcagtgagat ggcaaaccag tctgccatgt
     2401 agggggtttc aaatcgtttc atcgctgtcc tatgataacg aaaaagggat gtttcgacaa
     2461 catccccgac gttacagtca gattacacct
//
LOCUS       DAOREF010000472         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30674_length_2488_cov_3.961365, whole genome shotgun sequence.
ACCESSION   DAOREF010000472 DAOREF010000000
VERSION     DAOREF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30674_length_2488_cov_3.961365"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKI33_10105"
     CDS             <1..275
                     /locus_tag="PKI33_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37409.1"
                     /translation="LALSFLLLIGFTLVVEGLHQHIPKGYVYFAMGFSVMVEMINLRV
                     RGKSEPVALHTPYVAEPVAVSASAAISSPKKPAAKKAKSASKKSKK"
     gene            complement(305..1198)
                     /gene="thrB"
                     /locus_tag="PKI33_10110"
     CDS             complement(305..1198)
                     /gene="thrB"
                     /locus_tag="PKI33_10110"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNM37410.1"
                     /translation="MSRMIKIRVPATSANLGPGFDCLGLALDVWNEIAFEPAEQLKYR
                     ASGEGAEKLNKGNRNLLTKAYARLHEFCGKKASGVRIAARNDIPMSSGLGSSAAAIVA
                     GLFGANEMLGKPLHKDELVKLATEMEGHPDNVAPALLGGLVASVMKHEEIITRRYEVP
                     ALTVVVVKPDVEWLTKTARAVLPKSISRADAIFNIGRATLVVDALRTGDLDLLQKVMD
                     DRIHQPYRLRHISGGMAAYKCAKAFGAAALSGAGPSIILFTQPNQAERAKREIQAVFE
                     ERGINTKGIITSPGKKGVHCI"
     gene            1288..2322
                     /gene="thrC"
                     /locus_tag="PKI33_10115"
     CDS             1288..2322
                     /gene="thrC"
                     /locus_tag="PKI33_10115"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNM37411.1"
                     /translation="MTYQGLIHRYRQYLDLPAHTQIVSLNEGNTPLIPMPRLGREFGC
                     ELFIKFEGLNPTGSFKDRGMTAAISEAVGRGAKTVICASTGNTAASAAAYAARAGLRS
                     IVLIPEGKVAVGKLAGAIAYGAEVIQIQGSFDDALSLVVEITQKTPIALVNSINPYRI
                     EGQKTSPFEICDELGSAPDWLCLPVGNAGNITSYWAGFKQYGKGLPRVLGVQAEGAAP
                     LVLGHPVDKPETIATAIRIGRPARGEQALEAAHESNGKIMAVSDAQILDAQRILASEG
                     VWVEPASAAGVAGLIAETATGKLDAKGKRVVVVCTGHGMKDPSIITESFSAPQVIPAK
                     YEALVELVGS"
BASE COUNT          629 a          598 c          612 g          649 t
ORIGIN      
        1 ttttagcctt gagttttctg ttgttgatcg gttttaccct tgttgtagaa gggcttcatc
       61 agcacatacc aaagggttat gtatatttcg caatgggctt ctcagtgatg gtcgaaatga
      121 tcaacctccg tgttcgtggc aagtctgagc cagtggcgtt gcatacaccc tatgtagcag
      181 agcctgtcgc ggtttcagcc tctgcagcga tttcttctcc aaagaaaccg gctgccaaga
      241 aggcaaaatc tgccagcaag aagagtaaaa aataaaaaaa agcggacttg gaaaagtccg
      301 ctttttatat gcagtgaaca ccttttttgc cagggctggt aatgatccct tttgtgttta
      361 ttccacgttc ttcgaataca gcttggatct ctctcttggc gcgttctgcc tgattcggtt
      421 gcgtaaaaag aataatagaa ggacctgctc cagataaagc agccgcgccg aatgccttgg
      481 cgcatttgta tgctgccatt ccgccagaaa tatgacgcag acgataaggc tgatggatgc
      541 ggtcatccat cactttttgg aggaggtcaa ggtcaccagt acgaagcgca tccacaacca
      601 gtgttgctcg accgatatta aaaatagcat ctgcacgcga aatagacttt ggcaaaacag
      661 cccgagccgt tttagtcaac cattctacgt ctggtttgac gacaaccacg gtcaacgccg
      721 gaacttcgta tctgcgagtg atgatttctt catgcttcat caccgaagcg accaacccac
      781 caagcaaagc aggcgctaca ttatctggat gtccctccat ttctgtcgca agctttacca
      841 attcatcctt atgaagcggt ttgccgagca tttcatttgc gccaaagagt cctgccacaa
      901 tcgcggcggc gcttgagcca agcccactgc tcatcggaat gtcatttcga gcggcaatac
      961 gaactccgct tgcttttttg ccacaaaatt catgcaaacg tgcatacgcc tttgtgagca
     1021 ggttgcgatt ccctttgttt aacttttcag ccccttcacc gcttgcgcga tatttcaatt
     1081 gttcagcggg ctcaaatgca atttcattcc aaacatccaa tgccaaaccg aggcaatcaa
     1141 aaccaggacc aaggttcgca gatgtagctg ggacgcggat ctttatcatg cgggacatta
     1201 tataatacct gcgttgcaaa tgtggaaaaa tgtgcacttt gtaaccggga tgagtataat
     1261 cgcctcaatc tataactcgg agttgatatg acctaccagg gcttaatcca tcgctacaga
     1321 caatacctcg acctacccgc ccatacccaa attgtttcgc taaacgaagg gaatacccct
     1381 cttattccga tgccgcgcct agggcgtgaa tttggttgcg agttgttcat taaatttgaa
     1441 ggtttgaacc caaccggctc tttcaaggat cgcggtatga cagccgccat cagtgaagcg
     1501 gttgggcgcg gcgcaaagac cgtcatttgt gcttccactg gcaatacggc tgcgtctgcc
     1561 gctgcttacg ctgcccgggc tggtttgcga tccattgtgc taattcccga aggtaaggtg
     1621 gcagtaggga agttggcagg cgctattgcc tacggtgcgg aagtgattca aattcagggc
     1681 tcttttgatg acgccctatc tttggttgtg gagatcaccc aaaaaacacc gattgctcta
     1741 gtaaactcga tcaatccata tcgcattgaa ggacaaaaga cctcaccttt tgaaatttgt
     1801 gatgaattgg gttccgctcc agattggtta tgtttgcccg tcggcaatgc ggggaatatc
     1861 acatcttact gggcgggttt caagcaatat ggaaaaggtt tgcctcgcgt gcttggcgtt
     1921 caagctgagg gagccgctcc gctcgtgtta ggtcaccccg tggataagcc cgaaacaatt
     1981 gctactgcta ttcgtatagg tagaccggcg cgcggagaac aagcgctaga agccgcacac
     2041 gaatccaatg gaaagattat ggcagtatct gatgcgcaga ttttggatgc gcaacgaatt
     2101 ctggcttcgg aaggggtgtg ggtggaacca gcgtcagcgg cgggagtggc aggcttgatt
     2161 gcagaaacag ccaccggcaa gttggatgcg aagggcaagc gcgttgtggt ggtatgcacg
     2221 ggtcatggta tgaaagaccc atccattatc acggaatcgt tcagtgcgcc tcaagtcatc
     2281 cctgcgaagt acgaagcttt ggttgaactg gttggcagtt aaaaaatctt tatgaaagct
     2341 aggtttccta gtaaaatata gaagtaatgt ctaatcaacc tctcgtcatc gaaaacctca
     2401 cgttcaaata taaaacccgt cctgaattag ccttggagaa tgtttctctt gagctgaagc
     2461 agggcgagtt gttattaatt gcaggctc
//
LOCUS       DAOREF010000473         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30725_length_2485_cov_3.730864, whole genome shotgun sequence.
ACCESSION   DAOREF010000473 DAOREF010000000
VERSION     DAOREF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30725_length_2485_cov_3.730864"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PKI33_10120"
     CDS             complement(<1..827)
                     /locus_tag="PKI33_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNM37412.1"
                     /translation="MASFNERRPFCLNSKLCYKNGAMRLIRILLLGIFIFSLVPGIVS
                     AQTPTPPTAVRVILNSMTPEERVGQLFLVTFKGTNTGTDSQIYELITKRHVGGVVLTA
                     GNDNFSETDTLARAHQLIEDLQQVEWTNSANTAVDPVTGSLERYVYVPLFVGISQEGD
                     GYPTDQILNGLTPLPSEMAIGATWNTEFAQNVGEVRGQEIAALGFNIFLGPSLDVLET
                     PSSSGGDLGTRVFGGDPFWVGEMGRAYVNGLHTGSNGNMLVIAKHFPGAGGADRLPQ"
     gene            complement(880..1632)
                     /locus_tag="PKI33_10125"
     CDS             complement(880..1632)
                     /locus_tag="PKI33_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNM37413.1"
                     /translation="MLETFPRPILLAHRGDLAKAPENTLPAFSQAIQKGADGVELDAK
                     LTTDGHVIVIHDETVDRTTDGKGKVATFSLEAIRKLDAGSWFDSKFADTKVPLLEEVF
                     ETVGKDKLINIELTNYYTPRDGLVVKVCELIKRHNNAKQIIFSSFLPSNLKIAQQTLP
                     EIPRGLLALPGFMGLWARSFGFMFGDYQASHPHISDANRESIQRAHRVNRRVHVWTAN
                     SPQEIQRLRDWGVDGLFTDDPGTAVQALGRTR"
     gene            complement(1669..>2485)
                     /locus_tag="PKI33_10130"
     CDS             complement(1669..>2485)
                     /locus_tag="PKI33_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM37414.1"
                     /translation="MYGPPGNGKTSIARAFGNLVLSQNMYIPYALYLDGQVIKVYDAV
                     SHSLAPDNDVASNPTGSLRSNTRRDPRWVKIRRPFIVVGGELTLEGLDLVFDDTTKFY
                     EAPFQVKANGGILLIDDFGRQQVRPRDLLNRWIVPLENRMDFLRLHTGRKVEVPFDVL
                     IVFSTNLPPKDLVDEAFLRRLRHKIEIGDPSFEEYREIFKRVAQDKRIEYNDQGLAYL
                     LQEWYIKRNRKLRASHPRDLCDQILDISSYMAVPPSMSREMLDRAAKAYFVDI"
BASE COUNT          676 a          619 c          581 g          609 t
ORIGIN      
        1 tcttgcggta aacgatctgc tccgcctgcc cccggaaagt gcttggcgat caccagcata
       61 tttccatttg acccagtatg caaaccattg acataggcgc gacccatctc ccctacccaa
      121 aacgggtcac caccaaacac acgcgttccc aaatcaccgc ccgatgaaga tggtgtctct
      181 aacacatcca gcgatggacc aaggaaaata ttgaaaccta acgcagcaat ctcctgaccg
      241 cgaacttctc caacattttg cgcaaattcc gtgttccatg ttgccccaat tgccatttca
      301 ctaggcaaag gcgttaaccc attcagaatt tgatctgttg ggtagccatc cccttcctgt
      361 gaaataccaa caaacaaagg cacatacaca tatcgttcaa gagagccggt aactgggtct
      421 acagcggtgt tcgctgagtt ggtccactcc acctgctgaa ggtcttcgat caactggtgc
      481 gcacgtgcca gagtatcggt ctcgctaaag ttatcatttc cggctgtaag caccacgccg
      541 cccacatgcc gcttggtgat cagctcataa atctgtgaat ctgtcccggt attcgtacct
      601 ttaaacgtaa ccaaaaataa ctgcccaacc cgctcttcag gcgtcatcga atttaaaata
      661 actcgcaccg cagtaggcgg tgtaggcgtt tgtgcggaaa ctatccccgg cacaagcgag
      721 aagataaaaa tacccaagag aagaatacga attaagcgca ttgccccatt cttataacac
      781 aacttgctat taagacaaaa tggacgccgt tcattgaacg acgccattgg aacttcgctt
      841 caaaccgcgc tcgggcttgc tcccggcatt cggaaagtct cacctcgtcc gtccaagcgc
      901 ttgaactgct gttccggggt catctgtaaa gagcccatcc acgccccaat cgcgcaatcg
      961 ttgaatttct tgcggagagt ttgccgtcca cacatgcaca cggcggttga cacgatgtgc
     1021 ccgttgaata ctctcgcggt tcgcatcaga aatatgtgga tgagaggctt gataatcgcc
     1081 aaacataaag ccgaatgaac gcgcccataa tcccataaaa ccgggcaatg ccaaaagtcc
     1141 acgcggaatt tcagggagag tctgttgtgc aatctttaga ttcgaaggga gaaaagacga
     1201 aaaaataatc tgtttggcgt tgttatgacg cttaattaat tcacagacct ttaccactaa
     1261 cccatctcgc ggggtgtaat aattcgtcaa ttcgatattg atgagtttat cttttccgac
     1321 ggtttcaaag acttcttcca acaaaggtac ttttgtatct gcaaactttg agtcgaacca
     1381 tgaacctgca tctaatttac gaatggcttc aagagagaac gtagctactt tcccttttcc
     1441 atctgtagtg cggtcaaccg tctcgtcgtg aatgacaatt acatgaccat ctgtggttaa
     1501 tttagcgtcc agttctacgc cgtcggcgcc tttttgaata gcttgagaga acgccggcag
     1561 ggtgttctct ggcgctttag caaggtcacc gcgatgggca agaaggatag ggcgagggaa
     1621 agtttcaagc atggtaggca ggctcagggt ctgaaagagg cgttgagttt aaatgtccac
     1681 aaaataagct ttggcagctc ggtctagcat ttcacgagac atggaaggtg gaaccgccat
     1741 gtaggatgaa atatccagaa tctgatcgca cagatcacgc ggatgagagg cgcgaagctt
     1801 gcgattgcgt ttgatatacc attcttgtaa aaggtatgcc aaaccttggt cgttgtattc
     1861 aatacgctta tcttgggcta ctcgtttgaa aatttcacga tactcttcaa aagaagggtc
     1921 gccaatttcg atcttgtggc gcaagcgacg aaggaacgct tcatccacca agtcttttgg
     1981 cggaaggtta gttgagaaga cgatcaaaac gtcgaagggc acttccactt tgcgtccggt
     2041 atggaggcgg agaaaatcca tgcggttttc aagtggaacg atccaacggt ttagcaaatc
     2101 acgcgggcgc acttgttgac gtccaaagtc gtcgatcagc aaaatgccac cgttggcttt
     2161 tacttggaat ggcgcttcat aaaatttggt ggtgtcatca aaaacaaggt cgagaccttc
     2221 gagagtcaac tcgccaccta ctacaatgaa agggcggcga atttttaccc aacgtgggtc
     2281 acgacgagtg ttagaacgca aagaacctgt tgggttggat gccacgtcat tatcaggagc
     2341 aagagaatgg ctgactgcat cgtaaacttt aatcacctgc ccatccagat agagtgcgta
     2401 tggaatgtac atattctggc taaggacaag gttgccaaaa gcacgcgcaa tactggtttt
     2461 accattcccg ggaggtccgt acatg
//
LOCUS       DAOREF010000474         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30726_length_2485_cov_3.599588, whole genome shotgun sequence.
ACCESSION   DAOREF010000474 DAOREF010000000
VERSION     DAOREF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30726_length_2485_cov_3.599588"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PKI33_10135"
     CDS             complement(<1..578)
                     /locus_tag="PKI33_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNM37415.1"
                     /translation="MAKKNTAGILAAFSSALFLGMAPVFGKLAITEGFSPFAVVALRT
                     GFAAGIMLLVMMLFYRQYLYIFPVGLIGCILAGAINGLGSLFYYLALERLDASLGQLL
                     YSLYPFFVALWLILDHQPPSRLTIFRLALATIAVLLLTNVPDHPMDLTGVAMMIAAAV
                     LYAMHLPINQRVLYEVPAPTVALYTLISMSAIV"
     gene            complement(602..2257)
                     /locus_tag="PKI33_10140"
     CDS             complement(602..2257)
                     /locus_tag="PKI33_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNM37416.1"
                     /translation="MSFIIKCLDCGHNAPYFPTSTTCPKCGSQWREAEYDYEMLGKTL
                     PEKLKSRQQDLWRYRELLPVRNPNISLSLGEGYTPLIRAVNLGMMLGCPNIFIKDERQ
                     GPTSSFKDRQASVTIAALKEAGITEMVAASTGNVAISYSAYASRAGMKLWAFVTSLVP
                     AVKMREIALYGSQVIKITGSYDQCKQVAAEFARQRKLYLDMGARTITSIEAMKTIAFE
                     IAEQLTNVLGPDENSQLRTPDWYVQAISGGMGPIGVHKGFREMQQMGWVNKIPAFAPI
                     QAEGCAPMVDSWKKGLAKAEPVTNPKTRIETLATGDPGRAYEFLKGHVDSSNGVFESV
                     TDEEAFRAMHVLAKMEGISAEPAAGAAFAGLFKLIRAGVIKPSDTVVVNCTGHTMPAE
                     QGILGENWARDVKFPSLAQAPQEEGLLSALTQVAPERFPKIAIVEDTLEARRLIRRIL
                     QSQGNFTILEAEDGRAGLDLVQKELPDLVILDLMMPEMDGFSVIEALRSNPETATIPV
                     IVATAKELTPDEKNRLSGHIQALLQKGDFLNDEFLDEVRSLLK"
     gene            complement(2280..>2485)
                     /locus_tag="PKI33_10145"
     CDS             complement(2280..>2485)
                     /locus_tag="PKI33_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37417.1"
                     /translation="GIQILEQLQADKRLRDIPTIVISGMDVTPEQKNKLKEFGQRLLT
                     KGAFNESELLTSIQRSLDRLSKK"
BASE COUNT          656 a          595 c          590 g          644 t
ORIGIN      
        1 acaattgcgc tcatagatat caatgtgtaa agagcaactg tcggcgcagg gacttcgtac
       61 aacacacgtt gattaatggg aagatgcatg gcatataaca cagcggctgc gatcatcatc
      121 gctacgcctg ttaggtccat ggggtggtct ggaacatttg taaggagcag cacagcaatg
      181 gttgcaagtg cgaggcggaa aattgtcagg cggctgggcg gttgatggtc aagaatcaac
      241 caaagtgcaa caaagaacgg atagagagaa taaagcagtt gccccaagct ggcgtctaaa
      301 cgttccaagg caaggtagta aaacagcgac cctagtccat taatggcacc agcaaggata
      361 cagccgatta gacctacagg aaagatgtag agatactggc gatagaacaa catcatgacc
      421 agtaacatga tccccgctgc aaatccagtt cgtaaagcaa caaccgcaaa tggtgaaaaa
      481 ccttctgtga tggcaagttt accgaacaca ggtgccatgc caaggaaaag ggcggagcta
      541 aacgccgcaa ggatcccggc tgtatttttt tttgccaatc ttgccagcct ttattgaata
      601 gttactttaa gagagacctg acctcgtcca gaaattcgtc gttgagaaaa tcaccttttt
      661 gcagcaatgc ctggatgtgt ccgctgagcc gattcttttc atcgggtgtc aactcttttg
      721 ccgtcgcaac aatcacaggg atggttgcgg tttcaggatt cgaacgtaat gcttcgataa
      781 cagagaaccc gtccatctct ggcatcatca agtcgagaat aacaaggtcg ggcaattctt
      841 tttgaacaag gtctaacccg gcacgcccat cttctgcttc aagaattgtg aaattccctt
      901 gcgattgcaa aatgcggcga atcaagcggc gtgcttcaag cgtgtcttct acgatggcga
      961 tcttggggaa gcgttcaggt gctacttgcg ttaacgcaga tagtaagcct tcttcttgtg
     1021 gtgcctgagc taaagacggg aacttcacat ctcgtgccca gttctcacca agaatacctt
     1081 gttcagctgg catggtatgc ccagtgcagt taacaaccac cgtgtcactt ggcttgatca
     1141 cacctgcacg aattaattta aacaagccag caaacgcagc tccagcggca ggctctgcgg
     1201 aaataccttc catcttcgcg agcacgtgca tagcacggaa agcttcttca tcggtcacac
     1261 tttcgaaaac accgttactt gaatcaacat gccctttcaa aaattcgtag gcacgtccgg
     1321 ggtcgcctgt ggcaagggtt tcaatgcgag tttttgggtt ggtaaccggt tctgcctttg
     1381 caagaccctt tttccaacta tctaccatgg gcgcacagcc ctctgcttga atgggagcaa
     1441 aagcagggat tttattcacc cagcccattt gttgcatttc acgaaagcct ttgtggacgc
     1501 caatcggtcc catcccgccg ctaatggctt gcacatacca atccggggtg cgcagctgag
     1561 agttttcatc gggaccgaga acgttggtaa gctgttctgc gatttcaaat gcaatggtct
     1621 tcatcgcttc aatggatgtg atagtacgtg cacccatgtc cagatacagt ttgcgttgac
     1681 gtgcaaattc agcagccact tgtttgcatt ggtcatatga cccagtgatt ttgatgactt
     1741 ggctgccata caaagcgatt tcacgcatct taactgcagg aacaaggctg gtgacaaatg
     1801 cccaaagctt catccctgct cgagaagcgt aggcagaata tgaaatggca acattcccag
     1861 tcgatgcagc taccatttca gtgataccgg cttctttgag tgccgcaata gtcactgatg
     1921 cctgacggtc tttgaaagaa gaagtgggtc cttgtcgttc atctttgata aagatatttg
     1981 ggcagcccaa catcatcccc aagttgacgg cacgaatcaa tggggtgtag ccttcaccta
     2041 gcgataaact aatgtttggg ttacgaacag gaagtagctc tctataccgc caaaggtctt
     2101 gctggcgcga ctttaacttt tctggcaagg ttttgccaag catttcatag tcgtattccg
     2161 cttcgcgcca ttgactccca cacttgggac aggttgtgga agttggaaag tatggcgcgt
     2221 tatgcccaca atctaggcac ttaataataa atgacactta attctcctgt acacttcgat
     2281 tatttctttg acaagcgatc cagcgagcgt tgaatcgaag ttagtaattc actttcgtta
     2341 aaagctccct tggttaataa gcgttgacca aactctttga gcttattctt ttgttcaggt
     2401 gtgacatcca taccgctgat tacaatggtg ggaatatcgc gtaaacgctt atctgcctgt
     2461 aactgctcaa ggatttgaat cccgt
//
LOCUS       DAOREF010000475         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30855_length_2479_cov_5.714521, whole genome shotgun sequence.
ACCESSION   DAOREF010000475 DAOREF010000000
VERSION     DAOREF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30855_length_2479_cov_5.714521"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKI33_10150"
     CDS             complement(<1..910)
                     /locus_tag="PKI33_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNM37418.1"
                     /translation="MPNKKKGTIALPLEKDEIIKDYRLAYQSRQASLIGRREVLSGKA
                     KFGIFGDGKEIAQLAIARAFQKGDWRSGYYRDQTWMFMLGIMSIQEFFAQLYAHADAQ
                     YDPASGGRQMNAHFATRHLHPNGAWRAQTQLYNTSSDVSCTAAQMPRAVGLAYASVLY
                     RNIKELNNLKDFSINGNEVTFATIGNASTAEGHFWEAVNAIGVLKAPAIITVYDDGYG
                     ISVPNQFQMVKENIGTLLKGFERDPRTPHNKVQNGYDHYTVRAWDYSALIETYQTAAE
                     VAREYHIPALVHVVDVTQPQGHSTSGS"
     gene            complement(1044..1412)
                     /locus_tag="PKI33_10155"
     CDS             complement(1044..1412)
                     /locus_tag="PKI33_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37419.1"
                     /translation="MSEAKKVVIERKKGYLSVEYAKPYNIDDLLSFSKEALEISKAEG
                     YKKLFLDISKMPGRIKPMERYEVGVKAATLFRYKLKVAVLYKTEEINRFAETVALNRG
                     MNVKIFNTKEEAISWLEVEE"
     gene            complement(1445..>2479)
                     /locus_tag="PKI33_10160"
     CDS             complement(1445..>2479)
                     /locus_tag="PKI33_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNM37420.1"
                     /translation="NKRYHFNSGGTTDVAMTDLISEEAVWACTSCGACVDICPVGNEP
                     MRDILDIRRNLSMMESAFPKQLETAFKGMERNVNPWNVSQGDRMKWAEGMSVPTIEQN
                     AEPEILWWVGCAPATDSRAQKTAQAFAKILNTAGVNYAVLGKNEACTGDSARRAGRED
                     IFFGLASQNVEILNEVAPKRIVTTCPHCLHTIKNEYPAFGGNYQVIHHTQLINELVGA
                     GKISMNLEGDNMKITFHDPCYLGRHNKVTDAPRDALKSAGVETVEMPRNGAKSFCCGA
                     GGAQMWKEEEQGTARVNATRFAEAKSTGADTVAVGCPFCLTMMTDASKGDGGTIQVKD
                     VAEIVAERLK"
BASE COUNT          578 a          583 c          592 g          726 t
ORIGIN      
        1 ggctgccaga ggtggagtga ccctgcggct gtgtgacatc cacaacgtgc actagtgcag
       61 ggatgtgata ttcgcgcgca acctctgcag cggtttgata cgtttcaatg agtgcagaat
      121 aatcccaagc gcgaacggtg taatggtcat agccgttttg cactttgttg tgtggtgtgc
      181 gcgggtcacg ttcaaagcct ttcagcaatg tcccaatatt ttctttgacc atctgaaatt
      241 gatttggcac cgagatgcca tatccgtcat catagacggt gataatggct ggcgctttga
      301 gaactccaat cgcgttgacc gcctcccaaa aatgaccttc tgctgtagag gcgttgccga
      361 tggtggcaaa ggtcacttca ttgccgttga tcgaaaaatc tttgagattg tttaattctt
      421 tgatgttgcg atataagacc gatgcatacg ccaacccaac ggctctgggc atttgagcgg
      481 ctgtacaaga gacatcggat gaagtgttat agagttgggt ttgtgcacgc caagcaccat
      541 tggggtgaag gtgacgggtg gcaaaatgag cattcatttg cctgccgcca ctggcggggt
      601 cgtattgagc atcggcatgt gcgtataact gcgcaaagaa ttcttgaatg ctcatgatac
      661 caagcatgaa catccaggtc tggtcgcgat aatagcctga acgccaatcg cctttttgaa
      721 aagcacgagc aatggcgagt tgagcgatct ctttgccatc gccaaaaata ccaaattttg
      781 ctttgccact tagaacttca cgtctaccaa taagggatgc ctgcctgctt tgataggcaa
      841 gacggtaatc ttttatgatc tcatcttttt caaggggaag tgcaatcgta cccttcttct
      901 tgttgggcat gaatctctcc tgttgagttg attgttcaag gattataaca aaggaagagg
      961 ggaaaggttt ctcgctggat tttgattgtg aaaaaaaaga aaaagcggat agttttatcc
     1021 gctttttctt ttttttgttt tttctattct tcaacttcaa gccatgaaat ggcttcttct
     1081 tttgtattga agatttttac attcattccg cggttaaggg caacggtctc tgcaaagcgg
     1141 ttgatctctt cggtcttata taaaaccgcc actttgagct tatatcgaaa aagcgtagct
     1201 gcttttactc caacctcata gcgttccata ggcttgatcc tgccaggcat tttggaaata
     1261 tccaaaaata actttttata gccttcagcc ttggaaattt ccaatgcttc ttttgaaaaa
     1321 cttagaaggt catctatatt gtagggcttt gcatattcta cagacagata gccttttttt
     1381 ctttcaatga ctactttttt cgcttctgac atacagttga gtcggcgttt tcaaatttat
     1441 tcttctattt caatcgttcc gctacgatct ctgctacatc tttcacttgg atcgtgcccc
     1501 catctccttt ggaggcatcg gtcatcatcg taaggcagaa gggacagccc actgccacgg
     1561 tatcggctcc ggtggatttg gcttcggcaa agcgagtggc attgaccctt gccgtacctt
     1621 gttcttcttc tttccacatt tgtgcgccgc ctgcaccaca gcagaaagat ttagcaccat
     1681 tgcgtggcat ttccaccgtt tcaacaccag cagatttcag cgcatcacgc ggcgcatcag
     1741 tgaccttgtt gtggcgccct aaatagcatg ggtcatggaa ggtgatcttc atgttatcgc
     1801 cttcaaggtt catggagatt ttgcctgcgc ccacgagttc gttgatcaat tgcgtatggt
     1861 gaatcacctg atagtttcca ccgaaggcgg ggtattcgtt cttgatcgtg tgcagacaat
     1921 gcggacaagt cgtcacaatg cgttttggcg caacttcgtt caagatttct acattctgtg
     1981 aagccaaacc aaagaatata tcttcgcgtc cagcacggcg agccgagtca ccggtgcagg
     2041 cttcgttctt gcctagtacg gcgtagttaa cgccggcagt attcaaaatt tttgcaaaag
     2101 cttgcgccgt tttttgtgca cgcgagtctg tagctggggc acaaccgacc caccataaaa
     2161 tttcaggttc ggcgttttgt tcaatggtgg gcacgctcat gccttctgcc catttcatgc
     2221 ggtcaccctg cgaaacattc cacgggttga cgttgcgctc catacctttg aaggcggttt
     2281 ccaattgctt ggggaaggca gattccatca tggagaggtt gcggcgaata tcgaggatgt
     2341 cacgcatcgg ttcattgcca acgggacaga tgtccacaca ggcgccgcag cttgtacacg
     2401 cccacaccgc ctcttcgctg atgagatcag tcatggcgac atcggtcgta ccaccagagt
     2461 tgaagtgata acgtttgtt
//
LOCUS       DAOREF010000476         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_30954_length_2474_cov_3.768913, whole genome shotgun sequence.
ACCESSION   DAOREF010000476 DAOREF010000000
VERSION     DAOREF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30954_length_2474_cov_3.768913"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..516
                     /locus_tag="PKI33_10165"
     CDS             46..516
                     /locus_tag="PKI33_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNM37421.1"
                     /translation="MDVTLNWQGKMAFEGAGPSGFVQRLDSDASAGGDNSGARPMEMI
                     AIGLAGCTAMDVISILSKKQQKVADFQIKLHAERASDHPKVFTDSVIEYHVYGMDIDE
                     KSLVRAIELSAEKYCPAQAMLSKAFPIRLIYKIFDAETKSLLKEGEYQPKQKVA"
     gene            complement(530..1939)
                     /locus_tag="PKI33_10170"
     CDS             complement(530..1939)
                     /locus_tag="PKI33_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37422.1"
                     /translation="MPNNQTAASTSQPNNAVEQSAVATAAFPTRRPYAPGELVDYVAQ
                     TGDTLPTLALRFNTSVDEILEANSFIPRDATTMPQGMPMKIPIYYRALWGSAFQIIPD
                     HAFINGPTNIGFNTAAFLSTQTGWLSTYRAYVGGEWKTGAALVDYAAINYSISPKLLL
                     AILEYQGGALTQPAPPVNKNLLGLKRPFWDSPYLQLVLAANTLNNGYYGWRTGTLLEF
                     EDDDAILIRPDPWQNPGSVALQYYYSRMFTGNDYNRAIGPDGLYKTYATFFGNPWENQ
                     FDLIPGSLSQPELSLPFPGDQTWSFTGGPHTGWGTGFPFAALDFAPPAEQSGCVPAKP
                     ENFATAVADGLVVRSAQDGLALDLDRDGDERTGWVIFYLHLATDQRATLGADLKTGDM
                     IGYPSCEGGRSTGTHVHIARKYNGEWIPADSALPFVISGWVPHNGIEAYQGTLTKGGL
                     TVTACVCGDAYTAISANYP"
     gene            complement(2004..>2474)
                     /locus_tag="PKI33_10175"
     CDS             complement(2004..>2474)
                     /locus_tag="PKI33_10175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNM37423.1"
                     /translation="SAIDFAPAAAKSGCVPNDAWVVAAAPGLVIRSENGVVMVDLDGD
                     GYEQTGWDLLYLHLATKDRVPAGTYLELNDRIGHASCEGGVATGTHLHFARKFNGEWV
                     TADGPIPFVMSGWRVVAGSAPYKGQLVKGDQVVTADTLSQAWSNIIREKDEEDN"
BASE COUNT          653 a          541 c          647 g          633 t
ORIGIN      
        1 atctagaaaa atatgttcct gatgtgattg aggagtaaaa ataaaatgga tgtgacatta
       61 aattggcagg gcaaaatggc atttgaagga gcgggacctt ctggctttgt acaacgctta
      121 gattcagatg cttctgctgg cggcgataat agtggcgcac gccccatgga aatgattgct
      181 ataggtctgg ctgggtgtac agccatggat gtgatctcaa ttctttctaa gaagcaacaa
      241 aaggtggcag attttcaaat caaacttcat gcagagcgag ccagtgacca tcctaaagtt
      301 tttacagact cggtgattga gtaccatgtt tatggtatgg atattgatga aaagtccctt
      361 gtccgtgcta ttgaactttc ggcagaaaaa tattgccctg cacaagccat gctttccaaa
      421 gcttttccga ttcgattgat atataaaata tttgatgcag agacaaaatc tctgttgaag
      481 gaaggcgaat atcagccaaa acaaaaggtc gcgtaagcga ccttttgttt tatggataat
      541 ttgcactgat ggcggtataa gcatcaccgc atacacaggc agtgacagtt aatccgcctt
      601 tggtgagggt gccctgatag gcttctatgc cattgtgcgg cacccagcca ctaattacaa
      661 agggtaatgc gctatctgca ggaatccatt cgccgttgta ttttctggca atatgaacgt
      721 gtgtcccagt agaacgcccg ccttcacaag acgggtaacc aatcatgtcg cctgttttta
      781 agtctgcgcc gagcgtggcg cgttgatcgg tggcaagatg taaataaaaa attacccaac
      841 ctgtacgttc atcaccgtca cggtcgaggt cgagagccaa gccatcttga gcagaccgca
      901 cgaccaaccc gtcggctaca gcggtggcaa agttttcagg ttttgcaggt acacagcccg
      961 attgttcggc aggaggcgcg aagtccaacg ctgcgaatgg aaaacctgtg ccccagccag
     1021 tgtggggtcc acctgtgaag ctccaggttt gatctcctgg gaatggcaga gaaagttctg
     1081 gctgcgataa actgccggga atcagatcga attgattttc ccaagggttg ccaaagaaag
     1141 tggcatatgt tttataaagc ccatctggac caatcgctcg attgtaatca ttgcctgtga
     1201 acatacgtga gtaatagtat tgaagcgcaa ccgaacctgg gttttgccat gggtctgggc
     1261 ggatgagaat ggcatcgtca tcttcaaatt caagcagagt ccctgtgcgc cagccataat
     1321 atccgttgtt gagtgtgtta gcggcaagca caagttgtaa ataaggtgaa tcccagaagg
     1381 gacgcttcag cccaagcagg tttttgttaa cgggcggggc aggttgtgta agcgcaccgc
     1441 cttgatattc aagtattgcc aataataact tggggctaat gctatagtta attgccgcat
     1501 aatctacaag agcggcgcct gttttccatt ctccacctac ataggcgcgg taggtggaaa
     1561 gccagccggt ttgagttgaa agaaacgctg cggtgttgaa accaatattg gttggtccat
     1621 tgataaaggc atggtctggg atgatctgaa atgcactacc ccaaagcgcg cgatagtaga
     1681 tggggatctt cattggcatg ccttgtggca tggtggttgc atcacgggga ataaatgagt
     1741 tcgcttcaag aatttcatct acgctggtat tgaagcgtaa agcaagggtc ggtagggtat
     1801 ctccggtctg agctacataa tctactaatt cacccggggc gtatggacga cgagttggga
     1861 aagcagcagt tgccacagca gattgctcca ctgcattatt cggctgtgaa gtggatgcag
     1921 ccgtttgatt gtttggcaag gatgtacaag aggaaagaaa caagacagct ataagagtaa
     1981 ccgtctggat ggagtttttt attttagtta tcttcttcat ctttttcgcg gattatattt
     2041 gaccaagcct gactcaatgt atctgcagtg acgacttgat cgcctttgac aagttgacct
     2101 ttataagggg cgcttcctgc aacgacccgc caaccgctca ttacaaatgg aataggacca
     2161 tccgctgtga cccattcgcc attaaattta cgggcaaagt gaaggtgtgt tccggtggca
     2221 acaccgcctt cgcaagaagc atgaccgatc cggtcgttga gttcaaggta ggtgcctgca
     2281 gggacgcggt cttttgtagc caagtggaga tataaaaggt cccagccggt ttgttcatag
     2341 ccgtcgccat caaggtctac catcactaca ccattttctg agcgaatgac taaccccgga
     2401 gcggctgcga cgacccaggc atcatttgga acacagccag attttgctgc ggcgggagca
     2461 aagtcgatgg cgga
//
LOCUS       DAOREF010000477         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31033_length_2471_cov_3.529801, whole genome shotgun sequence.
ACCESSION   DAOREF010000477 DAOREF010000000
VERSION     DAOREF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31033_length_2471_cov_3.529801"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..2028)
                     /locus_tag="PKI33_10180"
     CDS             complement(124..2028)
                     /locus_tag="PKI33_10180"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNM37424.1"
                     /translation="MNLISPIVNRWIQDGKEDPDAFWEKAAKELYWFRVWDRVFEWNY
                     PNFRWFVGAQTNLSYNALDVHVKQGWGGHTALIYMNERDERRLFTYAKLLHDVEKIAA
                     ALRGMGISKGDRITIYMPTCPEAIMLMLATVRIGAIHSVVFAGFGEKALGDRIQASGS
                     RLVFTTDITYRKGKDVNLKSIVDGALAQVDNSVEHLVVLKRGSQDVPMNTPLDITWEE
                     FLEHSKGQPSDCVPMEANEPAFILATSGTTAKPKLAIHTHGGYQVHIASMARWVFGLK
                     PEDVWWATSDIGWIVGHSYIVYAPLIIGCTTIAFEGALDHPSSETNWKIAIEEFSVSG
                     VFTSPTAVRLLMRYGDEPLGRVNHDRVERIFCAGEVLNAPAWDWLQNKMLKNRIPVID
                     HMWQTETGGPVFGNPYGLGMLPIKPGSATIPLPGIQVEVMNMDGQPCAPNEKGIMVIK
                     RPFPGLTPALWGEPERYGNDYWNKIPNVYYTGDSAHMDADGYVWFAGRADEIIKIAGH
                     RIGTIEVETAFLKHPAVAESGVIGRPDEIRGEVISAFVLLKNGFQASEALKKELLETV
                     RHELGPVAVIGELNFVNMLPKTRSGKIMRRVLKAVTLDKDPGDITTIEDEGSVTEARE
                     AWQEMKNALS"
     gene            complement(2094..>2471)
                     /locus_tag="PKI33_10185"
     CDS             complement(2094..>2471)
                     /locus_tag="PKI33_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37425.1"
                     /translation="DAVNTPNPAYSPFMLGVLIFIQVLYVTMVYGPIAAFLVELFPTK
                     IRYTSMSLPYHIGNGVFGGLVPIIGLELITKTGNNFAGLWYPTAIAAICAVICYFKIP
                     ETYKVDIESDASVAAIGAKVSGD"
BASE COUNT          621 a          612 c          584 g          654 t
ORIGIN      
        1 atggataata gacttcgcct tccatttcct tcttcttcgc catgccagcc tccaaaagtg
       61 atatgtggat gttagcgggt attttaatcc gcttttctaa aattgcgtaa gagtgttatt
      121 catttaagat agggcgtttt tcatttcttg ccaggcttca cgagcttcag tgacggagcc
      181 ttcatcttca atcgtggtga tgtcaccggg gtctttatcg agggtcactg cttttagtac
      241 acgcctcatg atcttgccgg agcgggtttt gggcaacata ttgacgaagt ttaactcgcc
      301 gataactgcc acaggtccaa gctcgtggcg gacggtttca agcaattctt ttttcagcgc
      361 ctcagaagct tggaacccat tcttgagcaa tacaaaggct gagatgacct ctccacgaat
      421 ctcatcgggt ctgccgatga cgcccgactc tgctacggct gggtgcttga gaaatgccgt
      481 ttctacctcg atggtgccaa tgcgatgccc agcaatcttg atgatttcat ctgcccgccc
      541 tgcaaaccac acgtaaccat ctgcatccat atgagccgag tcgccagtgt agtagacgtt
      601 gggaatcttg ttccaataat cgtttccata tcgttctggt tcgccccata aagcaggagt
      661 taacccaggg aagggacgtt tgatgaccat gatgcctttt tcgtttggag cacatggctg
      721 accgtccata ttcattacct caacctgaat gccgggtagg ggaatggtcg ccgagccggg
      781 tttgatgggc aacatcccta agccgtaggg gttcccaaaa acagggccgc ccgtttcggt
      841 ttgccacata tggtcaataa ctgggatgcg attcttcaac atcttgttct gcaaccaatc
      901 ccaggcaggt gcgttgagga cttcgcctgc gcaaaagatg cgttctacgc gatcgtgatt
      961 gacacgacct agcggttcgt ctccatatcg catcaataaa cggacagcag ttggagatgt
     1021 gaaaaccccg ctaactgaaa attcttcaat ggcgatcttc caattcgttt cagaacttgg
     1081 atgatcgagc gccccttcaa aggcgatggt cgtacagccg atgatgagcg gcgcatagac
     1141 aatataactg tgtcccacga tccaaccgat atcagaggtt gcccaccata catcttcggg
     1201 ctttaatcca aagacccacc gtgccatgct ggcaatgtgg acttgatagc ctccatgagt
     1261 atgaatggct aattttggtt tggcagtggt accagaagta gcaaggatga atgccggttc
     1321 attggcttcc atgggaacac aatcgctagg ttgacccttg gagtgttcaa ggaattcttc
     1381 ccaagtaata tcgagcggag tattcatagg tacatcttga gaaccacgtt tcaagaccac
     1441 aagatgttcc acgctgttat ctacttgtgc caaggcgccg tcgacgatac tttttagatt
     1501 aacatctttc cccttgcggt atgtgatatc tgtcgtgaaa accaatctcg agccgctggc
     1561 ttggatgcgg tcacctaacg ctttttcgcc aaaacctgca aacactaccg agtgaattgc
     1621 accaatgcgg acggttgcta gcatcagcat aatggcttca gggcaggttg gcatataaat
     1681 agtaatgcgg tcgcccttgg aaatgcccat tccgcgtaag gcggcagcga ttttttcaac
     1741 gtcgtgaagc agctttgcat acgtaaagag gcgacgttca tcacgctcat tcatataaat
     1801 aagcgcggta tgacctcccc agccttgttt gacatgcaca tccaacgcgt tataagaaag
     1861 atttgtttgc gcccctacga accaacgaaa atttggataa ttccattcaa aaacacgatc
     1921 ccatacccga aaccaatata actcttttgc tgctttttcc caaaaggcat ctgggtcttc
     1981 tttgccatcc tgtatccaac gattaacgat tgggctgatc agattcatgt ctgtatctcc
     2041 attgaggcgg agcgacaaca tcgctccgcc tcaagatttg cttttatacg tgtttaatct
     2101 ccagagactt ttgcgccaat ggctgctaca gaagcatctg attcaatgtc aactttgtag
     2161 gtttcaggaa ttttgaagta gcagatcaca gcacagatcg ccgcaatggc tgtcgggtac
     2221 caaagcccgg cgaagttatt tccggtcttt gtgatcaatt ccaagccgat gatcggaacc
     2281 aagccgccaa agacgccatt gccgatgtga tacggaagcg acatggaggt gtagcgaatt
     2341 ttggtaggga agagttccac taagaaagcc gcaatcggtc catataccat ggtcacatag
     2401 agcacttgga tgaagatcaa cacaccaagc atgaagggac tatatgccgg gttgggcgta
     2461 ttgacagcat c
//
LOCUS       DAOREF010000478         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31066_length_2469_cov_4.852113, whole genome shotgun sequence.
ACCESSION   DAOREF010000478 DAOREF010000000
VERSION     DAOREF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31066_length_2469_cov_4.852113"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1295)
                     /gene="ftsZ"
                     /locus_tag="PKI33_10190"
     CDS             complement(120..1295)
                     /gene="ftsZ"
                     /locus_tag="PKI33_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNM37426.1"
                     /translation="MNPTNKNVQTEAFARIKVIGVGGAGQNAVNRMMEEGIQGVEFIA
                     ANSDAQALTLSRAPIRVRLGDKITRGLGAGGDPEVGRKAAEESSDELYNVLKGADMVF
                     VTAGMGGGTGTGAAPVVSQIAKECGALTIGVVTRPFTFEGGKRSQSAESGVAKMKEHA
                     HTLISIPNDRLLQLADKKSSLQDAFRMADEVLHQGIQGISELITIPGLINLDFADVRS
                     IMSEGGAALMAVGRGSGDDRAKVAAEQAISSQLLDITIDGARGVLFNVTGGPNMTLFE
                     VNQAAAIIRETAHPDVNMIFGAVIDPNMGDEIRCTVIATGFERSGATRRVLERLPRTN
                     LSEKPVSVANTPFQTRPTESVNAPVPETRSSADVKSQPLPSINNDDLDVPTFLRNRR"
     gene            complement(1427..>2469)
                     /gene="ftsA"
                     /locus_tag="PKI33_10195"
     CDS             complement(1427..>2469)
                     /gene="ftsA"
                     /locus_tag="PKI33_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNM37427.1"
                     /translation="SLEITTAFVSLARAHVSSINSRGSAGIPSGVIDIHDVARALEQA
                     QAIAIPHDREIIHVIQRGITVDGQEGVKTPVGMHGYKLEVETHIITAASATIDNIRQC
                     VNAAGVEVQQFVLNPLASAEVVLTEQERQMGVAVCDIGGGTTDLAIYVDGDVWHTMVL
                     AVGGNHITQDIAHGLRLSPSQAEEVKKQQGHALLSVIGPDEYFLMRPFGEDKDVRISR
                     QDLAHIIEARVEETFNLILQEIKRSGYDGLLPAGMVLTGGTSALLGINRVASEVLDMP
                     VRVAQPENLKGLVDKLNSPAYSTSFGLLQWAISMHDHQVTVSGGAKRRKPRGEGNVKF
                     DAIKDWMKRLLP"
BASE COUNT          602 a          635 c          628 g          604 t
ORIGIN      
        1 ggcgggtttg ctttttagac gttcagcgag atcggggagt ttatcgctca taaatctatt
       61 ttaccaaaat aaaagaccct aaaggtttgc aacctttagg gtctttgctt gtttagtgat
      121 tatcggcgat ttctcaagaa agtcgggacg tcaagatcat cgttgttgat gcttggcaaa
      181 ggctgagact tcacgtctgc tgaggaacgg gtttcaggaa ctggagcatt taccgattca
      241 gtggggcgag tctggaaagg agtgttcgcc acagagacgg gtttctcgct caggtttgtg
      301 cggggcaagc gctcgagaac gcgacgagta gcgccgctgc gttcgaagcc ggtggcaatc
      361 actgtacagc ggatttcgtc gcccatgttc gggtcgatca ctgcaccgaa gatcatgttt
      421 acatcggggt gagctgtctc gcgaatgatc gcagccgctt ggttgacttc gaagagcgtc
      481 atgttcggtc cgccggtgac gttgaagagc acgccacgag caccgtcgat ggtgatatcg
      541 agcaattggc tggagatggc ttgttcagca gccaccttgg cgcggtcatc gccagagccg
      601 cgacctactg ccataagagc cgcgccgcct tcagacatga tcgagcgcac atcagcaaag
      661 tcaaggttga tcaagccagg gatcgtgatc aactcggaga taccttgaat accctgatgc
      721 aacacttcgt ccgccatgcg gaaggcatct tgcagggatg atttcttatc agccagttgg
      781 agcaaacgat cattcggaat ggaaatgagc gtgtgcgcat gttctttcat cttcgcaaca
      841 ccagactcag cggactgtga acgtttgcca ccttcaaatg tgaagggacg ggtcacaaca
      901 ccgatggtga gcgcgccaca ttctttggcg atctgcgaaa cgacaggagc agcaccagtt
      961 ccggtaccgc cgcccatacc tgcggtgacg aagaccatgt ctgcgccttt gagcacattg
     1021 tagagttcgt ctgaggattc ttctgcagct ttgcgaccca cctcagggtc accgcctgcg
     1081 ccgagaccac gcgtgatctt atcgccgagg cgcacacgaa tgggagcgcg tgacagagtc
     1141 aaggcttggg catcagagtt ggcagcaata aattcaacgc cctgaatacc ttcctccatc
     1201 atgcgattaa cggcattttg accggcacca ccaacaccaa tcactttgat gcgggcgaaa
     1261 gcttcggtct gtacattttt attagtcgga ttcatttgtt cctcctggca aggaatatgc
     1321 ctaatcttaa acgagaatac gtttttttaa cagggttaat ttcgaccaaa attcacactt
     1381 tgtgaatctt ttctcatgat gtcaagggtt aaacagaatc tcgtcatcat ggtaacaacc
     1441 tcttcatcca atctttaatc gcgtcaaatt tgacgtttcc ttcaccgcgc ggcttgcgac
     1501 gtttagcgcc gccagacaca gtcacttgat ggtcgtgcat gctgatcgcc cattgcaaca
     1561 aaccaaagct tgttgaataa gctggtgaat tcaatttatc gaccaaccct tttaagttct
     1621 caggttgagc cacacgaacg ggcatatcca atacttcact tgcaactcga ttaatgccca
     1681 ataaagcaga agtaccgcca gtcaacacca tgccagcagg gagcagacca tcatacccag
     1741 agcgcttgat ctcttgcaag atgaggttaa aggtttcttc cacacgcgct tcgatgatat
     1801 gagccagatc ttggcggcta atgcgaacat ctttatcttc accgaaggga cgcatcagga
     1861 agtattcatc gggtccgatc acagatagca gggcgtgccc ttgctgtttt ttcacttcct
     1921 ctgcctgaga aggtgacaag cgtaatccgt gcgcaatatc ttgagtgatg tgattgccac
     1981 ccactgcaag aaccatggta tgccacacat cgccatcgac ataaatcgca aggtctgtgg
     2041 tgccaccgcc aatatcacaa acggctacac ccatctggcg ttcttgctcg gtcagcacaa
     2101 cttctgcaga agcgagcgga ttcaaaacaa attgttgaac ttccacaccg gcagcattca
     2161 cacactgacg gatattatca atggtggcgc tggcagcggt gataatgtgt gtttccactt
     2221 caagtttata accatgcatg ccaaccgggg tcttcacacc ttcttgtcca tctacagtga
     2281 tgccgcgttg gatgacgtga atgatctcgc gatcatgtgg gatggcaatg gcttgggctt
     2341 gttcaagggc acgggcaaca tcgtgaatat cgatcacacc gcttgggatg cccgccgagc
     2401 cacggctatt gatcgacgag acatgcgctc tggcaaggct gacaaaggca gtggtaatct
     2461 caaggctcg
//
LOCUS       DAOREF010000479         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31135_length_2466_cov_3.278308, whole genome shotgun sequence.
ACCESSION   DAOREF010000479 DAOREF010000000
VERSION     DAOREF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31135_length_2466_cov_3.278308"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1825)
                     /gene="pepF"
                     /locus_tag="PKI33_10200"
     CDS             complement(17..1825)
                     /gene="pepF"
                     /locus_tag="PKI33_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNM37428.1"
                     /translation="MTTPLPRSKVKKNQTWNAESVFKTPEAFDTELQNILADLPKIKK
                     YQGHLGENIEMFLEAFQAIDKLEKRTAKVRVYATMASAVDANDEQGAAMSSKAMSMLA
                     QVGAATSFVEPELLSIGEAKLRQWMKEDSRMKLYEHFLNDLFRKQAHVRNAEVEELLG
                     MVRDPFGAVRNTAGMLANADFKFKPAKDSKGRKLELTQSTRSALIESADRKVRQTSWE
                     NYNDKYLEFKNTLAGNLAASIKQNVFNMKARNFNSSLEATLFNGDVPVEMFHNLLDIF
                     KKKLPMWHKYWRIRRKALGVKVLHPYDVWAPLTTKKHKVPFETAVDWICEGLAPMGDE
                     YVNTMRKGCLEDRWVDWSPNAGKREGAFSTRVPSDTHPFIMMSYTDDVGSMSTLSHEL
                     GHSIHAWYASRAQPMMYYLYPSIIAETASNFNQAMTRAHLLKTKKDKYFQIALLEEAM
                     DNFHRYFFIMPTLARFELETHQRAEKGQSLTADYMNNLMADLFSEGFGGEMHLDRERV
                     GITWGTFTTHLYIDYYSFQYAIGISAANAIAKRVLDGVPGAAQDHINFLKAGSSMSPM
                     EVYKTAGIDMTSTQPIEDAFTVLEEYIDRIGELIEK"
     gene            complement(1938..>2466)
                     /locus_tag="PKI33_10205"
     CDS             complement(1938..>2466)
                     /locus_tag="PKI33_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37429.1"
                     /translation="ALMSPTAFKCGLENSTIAPGATLTCSNAYKTTQNDLAVANLQFS
                     TTASGTNVPTTTSVATTLTKGTAQLSLTVTPSATTYNTAGQTITFTYTIKNTGSGDLG
                     PTQFFINDPLMNPATLNCGAANTTLAPNATVQCTNNYTITDADLGAVNIQFSSSAGGT
                     GAPTSQSVPTTLTKQ"
BASE COUNT          553 a          535 c          645 g          733 t
ORIGIN      
        1 ccggctgtat gaaatgttat ttttcaatca attcgcctat acgatcaata tattcttcta
       61 atactgtaaa tgcatcttcg atgggttggg tggaagtcat gtcaatgccg gcggttttgt
      121 aaacctccat aggggacatg gaggaacccg ccttgagaaa gttgatatgg tcttgcgccg
      181 cgccggggac tccgtctagc acgcgtttgg caatggcgtt tgcagcggag attccgatag
      241 cgtattggaa ggagtaataa tcaatataaa gatgcgtggt gaatgttccc caagtgatac
      301 cgacccgttc ccggtcgagg tgcatctctc cgccaaagcc ttcgctgaat aggtctgcca
      361 tgaggttgtt catataatcg gctgtgagag actgtccctt ttcggcgcgt tgatgcgttt
      421 ccaattcgaa acgagcaaga gtgggcatga tgaaaaagta gcggtggaag ttatccatgg
      481 cttcttcgag tagagcgatc tggaagtatt tatctttttt tgtcttgagc aggtgggcgc
      541 gggtcattgc ctgattaaag ttcgaggcgg tctctgcaat gatggaggga tagagataat
      601 acatcatcgg ctgcgcacgg ctggcatacc aagcatgaat ggagtgaccc aattcatgcg
      661 agagcgtgct catcgagccg acatcatctg tatagctcat catgatgaaa ggatgtgtat
      721 cacttgggac gcgagtagag aatgcgcctt cacgtttgcc cgcattggga gaccaatcta
      781 cccaacggtc ttcaagacaa cctttgcgca tggtgttgac atattcatcg cccatgggcg
      841 cgagaccttc acagatccag tccacagcgg tttcgaatgg aactttgtgt ttctttgtag
      901 tcaacggcgc ccagacatca taagggtgaa ggacttttac acctaatgcc ttgcggcgaa
      961 tgcgccaata cttgtgccac atgggtagtt ttttcttaaa aatatccaat aggttatgga
     1021 acatttcgac cggaacatca ccattgaaaa gtgttgcctc cagtgaagag ttgaaattcc
     1081 gtgctttcat gttgaatacg ttttgtttga tcgaagcagc cagattccct gccagagtat
     1141 tcttgaattc aagatattta tcgttgtaat tttcccaaga agtttggcgc actttacggt
     1201 ctgctgactc aatgagagca cttcttgtgc tttgagtcaa ttcgagcttt ctgcctttgc
     1261 tgtctttggc gggcttgaat ttgaaatcgg catttgccaa cattccagcc gtattgcgga
     1321 ctgccccgaa cgggtcgcgt accatgccaa gcaattcttc cacttcggca ttacgcacat
     1381 gcgcttgctt gcggaataag tcattgagga agtgctcgta cagtttcatg cgcgagtctt
     1441 ctttcatcca ttgacgtaat ttcgcctcac ctatggacag caattccggc tctacaaatg
     1501 atgtcgctgc cccaacttgt gccagcatgg acatggcttt gctgctcatc gcagcgcctt
     1561 gctcgtcgtt tgcgtccacg gcgctcgcca tggtggcata cacgcgcacc tttgcagtgc
     1621 gcttttcgag cttatctatc gcttgaaacg cttcaagaaa catttcgatg ttttcaccaa
     1681 gatgcccttg gtattttttg atttttggaa ggtcagctag aatattttgc aattcagtat
     1741 cgaaggcttc aggcgtttta aagacacttt ctgcattcca agtttggttt ttctttactt
     1801 ttgagcgggg tagtggtgta gtcatgatac ggttcctttc aatgtgcgct gaaagataaa
     1861 taaaaatgta ctccgtattt ttagctatgt tgccaaaata tcggagtaca ttttgtcatc
     1921 ttcctgaaac gacagactta ctgcttggtt agcgtagttg gaacactttg cgatgtgggg
     1981 gcacctgtcc caccggcgct gctgctgaat tggatgttca cagcgcccaa atctgcgtcg
     2041 gtgatcgtat agttatttgt acactgcacg gttgcattgg gtgctagtgt ggtattggct
     2101 gcaccgcaat tcaatgtggc tggattcatc aatgggtcat taatgaagaa ttgcgtagga
     2161 cctagatctc cgctgccggt gttcttgatc gtgtaggtga aggtgatggt ctgcccagcg
     2221 gtgttgtatg tggtggcact cggtgttact gtaaggctga gttgagcagt tccttttgtg
     2281 agggtggtgg caactgaagt tgtagttggt acgttcgttc cagatgctgt tgtactgaat
     2341 tgaaggtttg ccactgccag gtcgttttgg gtggttttat aggcgttgga acaggtaagg
     2401 gtcgcgccgg gggcgatggt cgaattttcc aatccgcatt tgaaagcggt gggactcatc
     2461 aaggca
//
LOCUS       DAOREF010000480         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31157_length_2465_cov_3.734855, whole genome shotgun sequence.
ACCESSION   DAOREF010000480 DAOREF010000000
VERSION     DAOREF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31157_length_2465_cov_3.734855"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2465
                     /locus_tag="PKI33_10210"
     CDS             <1..>2465
                     /locus_tag="PKI33_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM37430.1"
                     /translation="GVLREEAVRISQADCASAVLFELPSSQTEPVIQLAVGCDRVPTV
                     TPPEWTVIKSGEPLVISDYAREAILPPHENAVSAVILPVMFQTRVLGLVTVHSNRAGF
                     FTAEAVELLQMLTSQAGIAVNNANRYQIEKQHSDLMRRRAETLVRLTDVSYNLGHDQP
                     LDQALQMIARAIRDATPFRVVLVSIFEAETSQLRRVTSVGLSQEAWNELSSRKQPLTS
                     VQQLMRSEFKISHSYFIPSDQTPIIPPDVHRVIVDASKTTTNNANTWQAEDFLLLPLE
                     NSEGQIVGLISVDDPSDGLRPDKATIEAVEVFAAQAALLISNTLRQTDLRSRIDSLSA
                     AFDRQQKLINMAKDDLPILLRKDLEQTISLHNLDRRAQRVRAGLAITESVSRQLDASS
                     ALSALGRETLTQLGMSVALVAENTPDGPRLLHVMGSLPRATNVEALFGQRNPMRSCLQ
                     NGMPILIPNLDEDDEWRDASLLTSLRAKGVICLPIIVEGKPVAAMLAASPEPMPSFTD
                     EDRHVYTQISQQTSVILQNISLLNQTRRRLDEVNLLLEFSRGLSGMDADSVVESLLAS
                     ARRVIPHAHAGVVLVWNSKTEHLVPRAVSGYADNDSMMKINYRLGEALPGTAFLNRKS
                     RRVDEINFPRDYNLNTENLAHYRQATGGRLPVSCLIVPIVSAEQNLGLLVLDNFNTTA
                     AFRPDDETLLVSLSQQVALSLDNMRLVQTSQERAGQLQALNDASASLTTSLSTDQLIQ
                     SLLDQLGPILPFDTATLWLRERDRLTVISTRGFSDVEERQGLSISVSDSALFKELAQT
                     GQPIFVKDVREDPRFPPVDAPRLS"
BASE COUNT          556 a          650 c          620 g          639 t
ORIGIN      
        1 ggtgttttaa gagaagaagc tgttcgtatc tcccaagccg attgcgcttc ggcagttctc
       61 tttgaattgc ccagttctca aaccgagcct gtcatccaat tggcagtggg ttgtgaccgc
      121 gttccgacag tgactcctcc tgaatggact gtaatcaaga gcggtgagcc gcttgtgatt
      181 tcagactatg cccgagaagc cattttgccg ccgcatgaaa atgcggtgtc tgcggttatt
      241 ttgccagtga tgttccagac tcgcgtgctt gggttggtca ccgttcactc aaaccgtgca
      301 ggctttttca cggctgaagc cgtggaactc ttgcaaatgc tcacttcgca ggcgggcatt
      361 gctgtcaaca atgcgaatcg atatcagatc gaaaaacaac acagtgacct aatgcgccgc
      421 cgtgcagaaa ccctcgtgcg tctgacggat gtcagctaca acttggggca tgatcaaccg
      481 ttagatcagg cattgcaaat gattgcacgc gccatccgcg atgctactcc gttccgtgtg
      541 gtattggtca gcatctttga agcagaaacc agtcaattgc gccgtgtcac atctgtggga
      601 ctttcgcaag aagcatggaa cgaactttcc tctcgcaaac agcctcttac cagcgtgcag
      661 caattaatgc gctctgagtt caagattagc cactcttact ttattccgtc tgaccaaact
      721 ccgatcatcc cgccggatgt tcatcgtgtg attgtggacg cttcgaaaac gactacaaac
      781 aacgccaaca cttggcaggc tgaagatttc cttttgttgc cacttgaaaa ttcagagggg
      841 cagatcgttg ggcttatcag cgtggatgat ccttctgatg ggttgcgccc agataaggcg
      901 acgattgaag cggtggaagt cttcgctgca caagccgcat tgcttattag caatacattg
      961 cgccagacgg atctacgttc ccgcatcgat tcgctttctg cggcatttga tcgccagcaa
     1021 aagctgatca atatggcgaa agatgatctg cccattctgc tccgcaaaga ccttgagcag
     1081 accatctcgc ttcataattt ggatcgacgt gctcaacgcg tccgcgcggg gttggcgatc
     1141 actgaatcag ttagccgcca attggatgca agctcggctc tttctgcgct cgggcgtgaa
     1201 actcttaccc aacttgggat gtctgttgcg ctggtcgctg aaaacacacc agacggtccg
     1261 cgtttgttgc atgtaatggg gagcctgcca cgtgctacca atgtggaagc attgttcggt
     1321 caacgcaacc cgatgcgctc ttgtttgcaa aatggtatgc ccattctgat tccgaacctt
     1381 gatgaggatg atgaatggcg cgatgcttca ttgcttacgt ctttgcgtgc caaaggcgtg
     1441 atctgtttgc ccatcatcgt ggaaggcaag ccagtggctg ccatgttggc ggcaagccca
     1501 gagccaatgc catcgttcac tgatgaagac cgccacgtat acacccagat ctctcagcag
     1561 acttcggtca ttctgcaaaa catttctctc ttgaaccaaa cccgccgcag gttagacgaa
     1621 gtgaacttgt tgcttgaatt cagccgtggc ttatctggca tggatgcaga ctcggtggtt
     1681 gagtctctgt tagcgagcgc tcgtcgcgtg atcccgcatg cacatgccgg tgttgttttg
     1741 gtttggaata gcaagacaga gcaccttgta cctcgtgcgg tttctggtta tgccgataac
     1801 gatagcatga tgaagatcaa ttatcgtctc ggtgaagcct tgcccggtac agcattcttg
     1861 aatcgaaaat cacgccgtgt ggatgagatc aacttcccgc gtgattacaa cctcaatact
     1921 gagaaccttg ctcattaccg tcaggcaacg ggcgggcgtt taccagtatc ttgtttgatc
     1981 gtgccgattg tttcagcaga acaaaatctg ggtctgctcg tactcgataa cttcaacacc
     2041 actgcagcct tccgccctga tgatgaaacc ttgttggtct cactttcaca acaagtggcg
     2101 ctctcgcttg acaatatgcg cttggtacaa acctctcaag agcgcgcagg tcaattacag
     2161 gcgttgaacg atgcgtcggc ttcgttgaca accagcttga gcacggatca gttgattcaa
     2221 tctctgctcg atcagcttgg accgatctta cctttcgata cggcgacgct ctggcttcga
     2281 gaacgcgacc gcctaaccgt gatttctact cgcggtttct cggatgtaga agaacgccaa
     2341 ggcttgtcca tctctgtgag tgacagtgcc ttgttcaaag agttagcgca gactggtcag
     2401 ccaatcttcg tcaaagatgt gcgtgaagac ccgcgcttcc cacctgtgga tgcaccacgc
     2461 ctttc
//
LOCUS       DAOREF010000481         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31162_length_2465_cov_2.965975, whole genome shotgun sequence.
ACCESSION   DAOREF010000481 DAOREF010000000
VERSION     DAOREF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31162_length_2465_cov_2.965975"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKI33_10215"
     CDS             <1..625
                     /locus_tag="PKI33_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HNM37431.1"
                     /translation="GVNTVEREGISFAHPARFILVGTMNPEEGDLRPQLLDRFALSVE
                     IVGIREARERMTIMERNISYERDPEAFRKQWQPKEDELSEQIAKARELVDQVKYTSRD
                     LLSIAALTASLNVDGHRADMVILKAARAEAAFEGRTKINDHDIALAAELTLPHRIKRT
                     PFQQAEMTTEQLQERIEQLAGQSMPNEEEDDSSESQEKGSDKEEKKT"
     gene            679..1590
                     /locus_tag="PKI33_10220"
     CDS             679..1590
                     /locus_tag="PKI33_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNM37432.1"
                     /translation="MENREVFASSNANWWEGGQKVKAGDTFQPRKLNTPLDKLTRKQA
                     GRRSLTKTERKHGRYIMARPAGDNLTDIAFDATIRAAAPFQKQRTEERKKLAFSIKRS
                     DLQRKIRVKRVSNLVLFLVDASWSMAVAERMNATKGAILSLLTDAYQRRDRVGLIVFQ
                     KDRATLVLSPTNSVQLAQRALMDIPVGGKTPLSAGLFMAHDVISHEKHIHPDVEPLLI
                     VLTDGAGNVSLGTLPPQEEGFKFAEMIAMEKIRSVVINMEHAAFDQGLAQQLANHLEA
                     PCFSLNELKAETLYNAVVDEMSKPKKK"
     gene            complement(1625..>2465)
                     /locus_tag="PKI33_10225"
     CDS             complement(1625..>2465)
                     /locus_tag="PKI33_10225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNM37433.1"
                     /translation="SNAMLNPARLAWGLKKVCLQLGVRFFEGTPVTGLEEKRESLILK
                     TPQGQVEARKVAMATNAFPPLLKYLRLYVVPVYDYVLMTEPLSKAQHDAIGWYGREGL
                     SDNANQFHYSQMTSDGRILWGGYDAIYHRNNAVAPQLENRPTSFGHLAEHFFQVFPQL
                     EGLRFTHAWGGVIDTSSRYTAFWGQAYHNKLAYAMGYTGLGVGASRFGARVMLDLLNG
                     ERTERTELQMVKSKPFPFPPEPFRSPIINFTRWSLDQADRNQGKRNLWLKTLDALGLG
                     FDS"
BASE COUNT          656 a          703 c          597 g          509 t
ORIGIN      
        1 gggcgtgaat acggtagagc gcgaaggtat ttcctttgcc caccccgctc gcttcattct
       61 cgttgggacg atgaatcctg aagaaggaga tcttcgccct cagttgctgg atcgcttcgc
      121 cctctcggtg gagatcgtcg gcatccgcga agcccgtgaa cgcatgacca tcatggaacg
      181 caacatttcg tacgaacgtg atcccgaagc cttccgcaag caatggcaac ccaaggaaga
      241 tgaactctcc gaacagatcg ccaaagcccg cgaactcgta gaccaggtca aatacacgag
      301 ccgcgatctg ctctccatcg ccgccctcac cgcctctctc aacgtggacg gtcaccgtgc
      361 agatatggtc atcctcaaag ccgcccgcgc cgaagccgca tttgaaggtc gcaccaagat
      421 caacgatcac gatattgccc tcgccgcaga attgaccctg ccgcaccgca tcaaacgcac
      481 gcccttccag caagccgaaa tgaccaccga acaactgcaa gaacgcattg agcagttggc
      541 gggtcagtcc atgccgaatg aagaggaaga tgactcttct gaaagccagg aaaaaggcag
      601 cgacaaagag gaaaaaaaaa cttaaaactg gaagatgagc aatccgaaca gggtccgcaa
      661 gaaggcaacc ccgaaccgat ggaaaatcgg gaagtgttcg caagcagtaa tgcgaactgg
      721 tgggaaggcg gacaaaaagt caaagctggc gataccttcc agccgcgcaa gctcaatact
      781 cctttagata aacttactcg caagcaggcg ggtcgtcgtt cgctcaccaa gaccgaacgc
      841 aaacatggtc gctacatcat ggcccgccct gctggcgata acttaacgga tatcgcgttc
      901 gatgcgacca tccgcgctgc agcccccttt caaaaacaac gaactgaaga acgcaaaaag
      961 ctcgctttct cgatcaagcg cagcgacttg caacgcaaga tccgcgtgaa gcgggtttca
     1021 aatcttgtct tattcctcgt agatgcttcc tggtctatgg cggtggctga acgtatgaac
     1081 gcgaccaaag gcgccatcct ttcgcttctc accgatgcct atcagcgccg tgatcgcgtg
     1141 ggtctcatcg tcttccagaa agaccgcgcc acactggttc tctcccccac caactccgtt
     1201 caactcgctc aacgcgccct gatggatatc cccgtcggcg gcaagactcc gctttcagca
     1261 ggcttgttca tggcacatga tgttatcagc catgagaagc atatccaccc ggatgtggag
     1321 ccgttgctga ttgtgttgac cgatggggct ggcaatgtct ctctcggcac cttaccgcca
     1381 caagaagaag gcttcaaatt cgctgaaatg atcgccatgg aaaaaatccg ctcggtggtg
     1441 atcaatatgg aacacgccgc cttcgatcag ggacttgccc aacaattggc aaaccatctc
     1501 gaagcgccct gtttctcgct caatgagcta aaagccgaaa cgctctacaa cgcggtggtc
     1561 gacgaaatgt ccaaaccgaa aaagaagtaa aaaagaggca gtcactagtg actgcctctt
     1621 tttattacga gtcaaacccc aaccccaatg catccaaggt tttcaaccaa agatttctct
     1681 tgccttggtt tcgatctgcc tgatccaatg accaacgcgt aaagttgata atcggagagc
     1741 ggaacggttc tggcgggaat gggaagggtt tgctctttac catttgcaat tcggttcgtt
     1801 cagtgcgttc gccattcaat agatctagca tcacgcgcgc accaaaacga gaagcaccca
     1861 ccccaagccc ggtgtagccc atagcatagg cgagtttatt gtgatacgcc tgcccccaaa
     1921 atgctgtata ccgagaactg gtatcgatca cgccacccca agcatgcgta aagcggagtc
     1981 cttccagctg aggaaagact tgaaagaagt gctccgctaa atgtccgaaa gaggtggggc
     2041 gattttcaag ctgtggtgcc actgcgttat tgcgatgata aatagcatcg tatccgcccc
     2101 acagaatccg cccatcggat gtcatttgag aataatgaaa ttgattggcg ttatcgctca
     2161 aaccttcgcg cccataccag ccgatggcat catgctgcgc ttttgaaagc ggttcggtca
     2221 tcaagacata atcatagacc ggcaccacat acaacctaag atatttaagc aaaggcggga
     2281 aagcattggt tgccatagct actttacgcg cttcaacttg accctgtgga gttttgagga
     2341 tgagcgactc acgcttctct tccaagccgg tcactggggt accctcaaaa aagcggacac
     2401 caagctggag gcacaccttc ttcaagcccc acgccaagcg tgctgggtta agcatagcat
     2461 tagac
//
LOCUS       DAOREF010000482         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31283_length_2459_cov_5.277038, whole genome shotgun sequence.
ACCESSION   DAOREF010000482 DAOREF010000000
VERSION     DAOREF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31283_length_2459_cov_5.277038"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..594
                     /locus_tag="PKI33_10230"
     CDS             169..594
                     /locus_tag="PKI33_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37434.1"
                     /translation="MTFLRKSVPFVLVAMSLATAVYQFAVSFSAGEWRNSSEEMVSKW
                     EDRIQSLCEALPVDVTQVGYIDDAAISEDPALFDANEFQLMQYSVAPVAIDSEKRHEW
                     MIGNFNDDKNLEVWLTEQLGAYELQGFGFGLYLIHDVEN"
     gene            595..1950
                     /locus_tag="PKI33_10235"
     CDS             595..1950
                     /locus_tag="PKI33_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37435.1"
                     /translation="MTSLLSFIVLLPRIFLGFFIVHSIWNVKDGKSLLVKIFLSAGVG
                     FGVSSLFSFLWIWVGLPLTVYAWVESILAILLMIWLLFQNREILRIPQVTVKQEFLWL
                     SFLAAGALFFALNLILFGLQYPHGRPDAWINWNVVARFIYLGGADWQATFLRQWDHPD
                     YPFLVPMTNAITWVLLGNASTWGPVALHFVLSLFAAGLLFAFVNIFRGFKQSVLSVLI
                     FTSLPFVVNQGMRQYSDFLVAYLILAAGGLTLMYLQTKEKRIAVLVGLVIGLAGWAKN
                     EGLMAILSVSFVWVLLALKKEGRTGLLRYVIGLVFPVVVIVLFKIFLAPSNDLMSAQE
                     SLADKIFDIERYKTIFSIAIPMLWNLGNTPISIIGLIILTALLVRRSTNRITGLWALA
                     VIVLIQLTAYFGIFVLTPRDLAWHLNTSLDRLYLHILPLAFLLFFLWLKSPQELLSKE
                     S"
     gene            1954..>2459
                     /locus_tag="PKI33_10240"
     CDS             1954..>2459
                     /locus_tag="PKI33_10240"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNM37436.1"
                     /translation="MLLTIDIGNTNLTLGLYEGDTLGAHWRLSTDHNRMPDEYGLQFL
                     GVLQNAGKTIKDIQGISVASVVPPLTGRVIQACREYLKQEPLVVDAGVKTGIKIRYED
                     PKAVGADRVCDAVAVMKLYGGPACVVDFGTATTFNAVTRDGEYLGGAITAGINLAAEA
                     LYTHAAKLP"
BASE COUNT          540 a          548 c          582 g          789 t
ORIGIN      
        1 ttcattgctt tggcattttc taaccttgca gaaaaatctt ggcttcagtg ggggcttgcc
       61 ataattttga tagctttaat ggtcaataat ttccacttca ttttctcgat ctttcagtct
      121 ctagctccct tttacgcctc tgttccatag accggttaaa atacctgcat gaccttcctg
      181 agaaaatctg tcccttttgt tttggtagcc atgtcgcttg caacggcggt ctatcaattt
      241 gctgtgagtt tttccgcggg cgagtggaga aattcttcag aggagatggt ctccaaatgg
      301 gaggatcgga ttcagtccct gtgtgaggcg ttgcctgtgg atgtgactca agttgggtac
      361 attgacgatg ccgcgatcag cgaagatccc gcgttgtttg atgccaatga attccaattg
      421 atgcaatatt cggtggcacc ggttgcaatt gattcagaga agcgtcacga gtggatgatc
      481 ggtaatttca acgatgataa aaaccttgaa gtatggctga cggagcaatt aggggcatat
      541 gaacttcaag gctttgggtt tggcttgtat cttattcacg atgtggaaaa ttagatgacg
      601 tcgcttctgt cattcattgt tttgctgcca agaatctttt taggcttttt catcgtccat
      661 tccatttgga atgtgaaaga tggcaaaagc ctgctggtta aaatctttct gtctgccggg
      721 gtggggtttg gcgtttcatc tctctttagc tttttatgga tatgggtcgg acttccatta
      781 acggtctatg cttgggtcga aagcattctt gcaatccttc tgatgatttg gctcctcttc
      841 caaaaccgcg aaattttgcg tataccacag gtcactgtga aacaagaatt tctttggctt
      901 tcatttcttg ctgctggtgc tcttttcttt gcgttgaatc tgattctttt cggattgcaa
      961 tacccacatg ggcgccccga tgcgtggatc aactggaacg tggtcgcgcg tttcatctac
     1021 ctcggcggtg cggattggca agcgacattt cttcgccaat gggatcaccc cgattatccg
     1081 tttttggtgc caatgacgaa cgccattact tgggtgcttt taggcaacgc atccacttgg
     1141 ggaccagtcg ctttacactt cgtattgtct ctctttgcag caggattgtt gttcgcattt
     1201 gtaaacattt ttcgcggctt taaacaatcc gttttatcgg ttcttatatt cacttcgctt
     1261 ccatttgttg ttaaccaggg aatgcgtcag tattcagact ttctggtcgc ttatctcatc
     1321 ttagcggcag gcggcttgac cttaatgtac ctgcaaacaa aagagaaacg gattgccgtc
     1381 cttgttggtc ttgtgattgg gttggcaggc tgggcaaaga acgaaggact aatggcgatc
     1441 ctgtctgtgt cttttgtatg ggttttattg gctttgaaaa aagagggacg aactggatta
     1501 ttaagatatg ttatcggttt ggttttccct gttgtagtga ttgtgctctt taagattttc
     1561 cttgcccctt ccaatgactt gatgtctgct caagaaagct tggcggataa gattttcgat
     1621 atcgagcgtt acaaaaccat ctttagtatt gcaatcccca tgctgtggaa tctggggaat
     1681 acgcctatca gcataattgg acttataatt ttgaccgcac ttcttgttcg tagatcaaca
     1741 aatcgtatta caggtctatg ggcgcttgct gttatcgttc ttattcagtt gacagcctat
     1801 ttcggaattt ttgtccttac tcctcgtgat ctggcttggc acctgaatac ttctttagat
     1861 cgtttgtatt tacacatcct tccacttgca tttcttttgt tcttcctctg gctgaaatct
     1921 ccgcaagaac ttttatcaaa agagagttga cccatgcttc ttacgatcga cattggcaat
     1981 accaacctta cgcttggttt atacgaaggt gatacgcttg gcgcacactg gcggctctct
     2041 acagatcaca accgtatgcc cgatgaatac ggcttacagt ttttgggcgt gttgcaaaat
     2101 gccggaaaga ccatcaaaga tattcaaggc atttcagtcg catccgtcgt cccgccgttg
     2161 accgggcgtg tgattcaagc ctgccgcgaa tatcttaaac aagaaccact cgtcgtggat
     2221 gcgggtgtga agaccggcat caagatccgt tatgaagacc ccaaagctgt cggtgcggac
     2281 cgcgtttgtg acgcagtggc tgtgatgaaa ctttacggcg gtcccgcctg tgtggtggac
     2341 ttcggcactg cgaccacctt caatgcagtt accagagatg gcgaatatct tggtggcgcc
     2401 atcacagcag ggatcaatct cgctgccgaa gcgctttata cgcatgctgc caaacttcc
//
LOCUS       DAOREF010000483         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31354_length_2456_cov_4.239067, whole genome shotgun sequence.
ACCESSION   DAOREF010000483 DAOREF010000000
VERSION     DAOREF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31354_length_2456_cov_4.239067"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1048
                     /locus_tag="PKI33_10245"
     CDS             89..1048
                     /locus_tag="PKI33_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNM37437.1"
                     /translation="MLRSFFIYLSKAAWAQKLITGWSFSWRIASRFVAGVTLTDAIKI
                     VRELNAKGINATLDHLGEHTNTPEEAQQAADSIFATLDAIGADPSLRSNISIKLTQIG
                     MGLDESLCAETLERILARAKTAKTFVRVDMEDTPYTDKTINLHYAMREKGYENHGMVI
                     QAYLFRTEADVRRMLQDGVRIRNVKGAYNEPPDKAFPQKADVDANYDLITKILLDASL
                     AQKSKLSDDGRVPPIPAIATHDERRIAFAKSYAEKIGLPKNGMEFQMLYGIRRDLQES
                     LVKEGYLVRVYVPFGTHWYPYFMRRLAERPANVWFFIVNFFKG"
     gene            1205..1405
                     /locus_tag="PKI33_10250"
     CDS             1205..1405
                     /locus_tag="PKI33_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37438.1"
                     /translation="MFFSHIALSPVFYLDPGSGSILFQMIVAALLGAGILIRSQWSRI
                     KRWFGVKPEVDENEDNEDNGGN"
     gene            1512..>2456
                     /locus_tag="PKI33_10255"
     CDS             1512..>2456
                     /locus_tag="PKI33_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37439.1"
                     /translation="MSSGLYESLIKSRLLVAHEEVNDIQPAEPKDAFKIIRPQRVNFI
                     SYPYEWSFSQLKDAALATLSIQKRAMKYGMSLKDASAYNIQFHEGKATLIDTLSFEKY
                     TEGTPWVAYKQFCQHFLAPLALMAKTDIRLSQLLRIHIDGIPLDLASGLLPFSTRLNF
                     GLLTHIHLHARAQQQYADKQVETSTAQKGGMSKQALQGLVESLESTVKALTWKPIGTE
                     WGDYYNFTNYSDSSFEQKKEIIGKWVEQVKPRSVWDMGANTGFFTRIASERGIPSIAF
                     DIDPAAVEKNYRQVKTSKEKNILPLIQDLTNPSPSIGWN"
BASE COUNT          706 a          504 c          548 g          698 t
ORIGIN      
        1 aatcacggaa aaagtataac ataccgaaac atagggcttg tgctagaata agttgatatt
       61 aatctaaatc acagttcaaa ggatggatat gctccgttca ttttttatct acctttcgaa
      121 agccgcctgg gcacaaaagc tgatcacagg ttggagcttt tcctggcgga tcgcatctcg
      181 ttttgttgcg ggggttactc ttacggacgc aattaaaatt gtccgtgagt taaatgcaaa
      241 ggggatcaat gctactttgg atcacttagg ggagcatacg aatacccctg aagaggcgca
      301 gcaggcagcg gatagtatct tcgcaacatt ggatgctatt ggagcggacc ctagccttcg
      361 cagtaatatt tccattaagc tgacacaaat cggcatgggg ctggacgaga gcctgtgtgc
      421 tgaaaccttg gagcgtatcc tcgctcgtgc taaaaccgct aaaacctttg tccgcgttga
      481 tatggaagat acgccgtata cggataaaac gattaacttg cattatgcca tgcgtgaaaa
      541 gggatatgaa aatcatggca tggttattca agcatatctc tttcgcaccg aagcggacgt
      601 tcgccgtatg ttacaagacg gggtccgcat tcgcaatgtg aaaggtgctt acaacgagcc
      661 acctgataaa gccttccctc aaaaagcaga tgttgatgca aactacgacc taatcacgaa
      721 gattttatta gatgcttcgc tggcacaaaa atcgaagctc agcgacgatg ggcgtgtgcc
      781 gcctatcccg gcgattgcga cgcatgatga aaggcgtatt gcttttgcca aaagctatgc
      841 tgaaaaaata gggctgccta aaaatggaat ggaattccaa atgttgtacg gtattcgcag
      901 agatttgcaa gagtctcttg taaaagaagg atatctggtt cgtgtttatg ttccgtttgg
      961 gacgcactgg tacccctact ttatgagacg cctggcagaa cgcccagcga acgtgtggtt
     1021 ctttatcgtc aacttcttta agggctaaaa gactcatcaa agcggtgaaa atctacagat
     1081 tttcaccgct ttttttgttt aaaagctggt tacgctttta cagaacatta accttattga
     1141 cgccatgatg tccctgcaat aaaataaaca gtcttgacat tatttcacca taaggaaagt
     1201 aaacatgttt ttctctcata ttgctctatc tcctgtattt tatcttgacc ctggatctgg
     1261 cagcatcttg ttccaaatga tcgtagctgc tcttttgggg gcaggtattt tgatacgctc
     1321 tcagtggtct cgcattaagc gttggtttgg cgtcaaacct gaagttgatg aaaacgaaga
     1381 caacgaggac aatggtggaa attaatcgta ttgacggctc tttccgcgac ccaagcggat
     1441 ttctctttaa gcacgatcaa gttttgtatc gtcaggtcaa taagagatat gctgatgaat
     1501 atactctttt gatgagttct ggtttatatg agagcttaat caagagtaga ttgcttgttg
     1561 ctcatgaaga agtaaatgat attcaaccag cagagccaaa agatgctttc aagatcattc
     1621 gtccacagag agtgaatttt atttcttatc cctatgaatg gtctttttcg caactaaaag
     1681 atgctgcact tgcgaccttg tctattcaaa aacgtgccat gaaatatggc atgtctctta
     1741 aggatgcgag tgcttataac attcaattcc atgaaggaaa agcaacctta attgatacac
     1801 tttcctttga aaagtacact gaaggtacac cttgggttgc ttataaacaa ttttgccagc
     1861 attttctcgc tccactggct ttgatggcaa aaacggatat tcgattatct caattattgc
     1921 gcattcatat tgacggcatt cctcttgacc ttgcaagtgg actcctgcct ttttcaacac
     1981 gcctgaattt tggattgttg acacatattc atttgcacgc tagagcgcag caacaatatg
     2041 cggacaagca ggtggaaact tccactgctc aaaagggcgg catgagcaaa caagctttac
     2101 aagggttagt tgaaagtttg gaaagtactg ttaaagcatt gacttggaag ccaatcggta
     2161 cagagtgggg agattactat aatttcacga attattctga ttcttctttt gaacaaaaga
     2221 aagaaattat tggaaagtgg gtggaacagg tcaaaccacg ttcggtctgg gatatgggag
     2281 ccaatacagg ctttttcacc cgcatcgctt cagaacgagg gattccttcc attgcctttg
     2341 acattgatcc cgcggctgtg gaaaagaatt atcggcaagt aaagacttct aaagaaaaaa
     2401 atattcttcc gctgatacaa gatctaacga atccgagccc atccattggc tggaac
//
LOCUS       DAOREF010000484         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31359_length_2456_cov_3.700125, whole genome shotgun sequence.
ACCESSION   DAOREF010000484 DAOREF010000000
VERSION     DAOREF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31359_length_2456_cov_3.700125"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKI33_10260"
     CDS             complement(<1..317)
                     /locus_tag="PKI33_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNM37440.1"
                     /translation="MEFKLRAPFQPMGDQPEAIRGLVDGVNRGLKHQVLLGATGTGKT
                     FTIASVIQQLQKPALIMAHNKTLAAQLYAEFKEFFPENAVSYFVSYYDYYQPEAYVPR
                     HDL"
     gene            452..895
                     /locus_tag="PKI33_10265"
     CDS             452..895
                     /locus_tag="PKI33_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37441.1"
                     /translation="MKTSPLTPEQQNWLKANAILPVVFLVIILAVFGGIFACLFSGLN
                     GKLFFTAFFAIAGFMIFAVLIAAGIHVYNNFMDLRDGVTQVHEAELMRKHHTYRSPKT
                     FYAEFEGVGSVIVMGDVYEKLEEGKTYRVIYSPRTRRGWDVDLRS"
     gene            complement(967..1905)
                     /locus_tag="PKI33_10270"
     CDS             complement(967..1905)
                     /locus_tag="PKI33_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNM37442.1"
                     /translation="MRRKTLLGLSIILLFMFACVPGLAAPTQDVDLIVQSTLQALTLQ
                     ALQSTPETAQSTPAASDQTGSISGKLSYPSSGIPPLLVVAFSLNNVDYYWVQTAQNQS
                     TYQLDGLPPGTYHVVAYALPDGKLAGRYDQFYVCGLAQGCNDTNWVDVQVQAGVVTPN
                     VDPGNWYGGAELYPPMPSYEAGQPSGKGIYSAPMPALGSISGKLSYPSSFIPSMTVVA
                     LNVKTSDYYYVITNENSGTYQIDNLPPGSYYVVAYPGNDSTYPGGYSQAVPCGLDASC
                     SDHSLIPVDVSGQLVTGIDPGDFYAPPGTFPASPVQ"
     gene            complement(1945..>2456)
                     /locus_tag="PKI33_10275"
     CDS             complement(1945..>2456)
                     /locus_tag="PKI33_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37443.1"
                     /translation="LRRIASEVYKVETDKFDTAFSTFNLPLSIPVNVDLPLAVGIPAS
                     YIPDQDLRLRLYRRIADLRDETELDALGSEFRDRFGQLPEMVQNLFYQIRVKLRAEKI
                     GLTSVSWESGQILLRYPAPVEGAEPKRLPDLPNGVRGGKGQYWVTLAKEEVWTAKLLD
                     VLARLGDVS"
BASE COUNT          683 a          570 c          602 g          601 t
ORIGIN      
        1 aacaaatcat gcctcggaac atatgcctct ggctgatagt aatcgtaata cgatacaaaa
       61 taagaaactg cattttcagg gaagaactct ttaaactctg cgtacaactg tgcagctaag
      121 gttttattat gcgccatgat aagggcaggt ttctggagtt gctgaataac agacgcgatt
      181 gtgaacgttt taccggtccc tgttgcgccg agcaagacct gatgcttaag accacggttc
      241 acgccatcca caagtccgcg aatggcttcg ggctggtcgc ccatcggttg gaagggagct
      301 ctgagtttaa attccataag tttatctttt cggtattatg tgaacgagtg ttctatttta
      361 cctcaacaca gccaaggtgt gtggaaactg acagccattg cacaagcatg ggacaagaaa
      421 tcaccacggg tataatttaa agcatataaa tatgaaaaca agcccactca cccctgagca
      481 acaaaactgg ttgaaggcaa atgcaattct gccagttgtc tttcttgtga tcatactagc
      541 tgtctttggt gggatatttg cttgtctgtt ctctggacta aatgggaaac tatttttcac
      601 cgccttcttt gccattgccg gctttatgat atttgcagtt cttattgcag caggaattca
      661 tgtgtataac aattttatgg atcttcgcga tggtgtgaca caagttcatg aggcggaact
      721 gatgcgcaaa catcatacct atcgctctcc aaaaacgttt tacgcggagt tcgaaggggt
      781 tggttctgta atcgtcatgg gcgatgttta cgaaaagctt gaagaaggca agacctatcg
      841 cgtaatctac agtccacgta cgaggcgtgg atgggatgta gatttgcggt cttaagaaaa
      901 atcgccccgt gagtttttgc tcacggggcg aaaatttttc agaaaaccga atcaagtagc
      961 ggctagctat tgaaccggag aagcgggaaa ggttccaggt ggggcgtaga agtcgccggg
     1021 gtcgatgcct gtcacaagtt gaccggatac atccactgga attaaagaat ggtcagagca
     1081 ggatgcatct aaaccacaag gcacagcctg cgaataaccg ccggggtaag tggaatcatt
     1141 tccgggatac gccacgacat aataagaacc gggcggaagg ttatcgattt gataggtgcc
     1201 cgaattttca ttggtgatga cgtagtaata atcagacgtt tttacattga gagccaccac
     1261 ggtcatcgaa gggataaagc tcgaagggta gcttagcttg ccagaaatac tacccaaggc
     1321 tggcatgggc gcagagtaaa tgcctttgcc gctgggttga cccgcttcat aacttggcat
     1381 gggtgggtag agttctgccc cgccatacca attaccagga tctacattgg gagtaacaac
     1441 gcccgcctga acttgcacat ccacccagtt ggtatcgtta cagccttgcg ctaaaccgca
     1501 aacataaaat tgatcgtagc gccctgcaag tttgccatcg ggcaacgcat aagcaactac
     1561 atgatacgtc ccgggtggca aaccatccag ttggtatgtg gattggtttt gagcggtttg
     1621 aacccagtaa taatcgacat tgttgaggct gaatgccacc accagtaagg gaggaatacc
     1681 cgaagatgga tacgataatt ttccagagat cgagccggtt tgatctgaag ccgcaggtgt
     1741 agactgtgca gtctctggcg tggattgcaa cgcctgtaag gtcaacgctt gcagggtaga
     1801 ttgcacgatc aagtcaacat cctgtgtcgg cgctgccaga cccggcacac aagcaaacat
     1861 aaagagcaaa ataattgaca agcctaataa agtttttctt cgcatctcat atctcctatt
     1921 tatgaattaa cccacacaaa caaatcagga gacatccccc aagcgggcta gaacatccaa
     1981 aagtttagca gtccagactt cttcctttgc gagcgtcacc cagtattgac ctttgccacc
     2041 tctgacacca ttcggcaaat caggcagacg cttcggctct gcaccttcaa cgggtgccgg
     2101 ataccgaagc agaatctgcc ccgattccca gctcacggag gtgagaccta tcttttcagc
     2161 ccgcaatttg actcgaattt ggtaaaagag attctgtacc atctctggca gttgaccgaa
     2221 acgatcgcgg aattcagagc caagcgcatc aagctcggtt tcatcgcgca gatccgcaat
     2281 acgacgatac aagcgcagac gcaggtcttg gtcgggaatg tagctggcag ggatgccaac
     2341 cgccaaaggc aaatctacat tcaccggaat ggaaagcggc agattgaatg tggagaaggc
     2401 agtgtcaaat ttatcggttt caactttata cacttctgaa gcaatacgcc gcaaac
//
LOCUS       DAOREF010000485         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31372_length_2455_cov_4.242500, whole genome shotgun sequence.
ACCESSION   DAOREF010000485 DAOREF010000000
VERSION     DAOREF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31372_length_2455_cov_4.242500"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /gene="mreC"
                     /locus_tag="PKI33_10280"
     CDS             <1..612
                     /gene="mreC"
                     /locus_tag="PKI33_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNM37444.1"
                     /translation="QLQAQLIELQQRVNETEILAALVDFSRSNPESTYKAASVIGRDP
                     SPFLHYIIINRGSNDDIRRGMPVVTNQGLVGRVDAVIADAARVQLITDPSSSVNVYLQ
                     NADTSAVLYGSVTGDVSLDLISQNASVEAGDLILTSGLGGGYPADLLIGQVVTVRSLE
                     FELFQQATVQPAVDFSRLEIVLVITNFRPVDIAPLEPVVTPSP"
     gene            619..1113
                     /locus_tag="PKI33_10285"
     CDS             619..1113
                     /locus_tag="PKI33_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37445.1"
                     /translation="MRNLIAFPLIGLAVILQSAVVSQVSLLSGYADLPLVLLAAWALQ
                     DRVDSSWHWGVLTCLFAGFISGLPISVVIAGYLGIILFARILQRRVWQAPLLAMFSVV
                     FLGTLFFHILSFVVLRFSGTPFAFGDVIGLITLPSLLLNMLISIPIYAFMRDVSYWVY
                     PVKE"
     gene            1116..>2455
                     /locus_tag="PKI33_10290"
     CDS             1116..>2455
                     /locus_tag="PKI33_10290"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNM37446.1"
                     /translation="MSSSAESRPVRFEGWRLGIIYVVVSLTLIGFVIRLVNLQIFNVD
                     QYEARAVENYAKPISVPAPRGIIYDRNGYILARNIAAYNVVITPASLPAEDSDIQRIY
                     RELSQLIDVPVGNFVANDPESNEIFLSVPGGLLAETYLEEAKLFASCVPGPSIGQLVA
                     LQDTLAPYSAVKIKCNVSEEIARIVEEKKMDWPGVSVEVEPIRDYPTGSLTSHLIGFL
                     GPIPASQEEELKALGFDPNRDKVGYAGVETSLQEILAGRNGLRVVQVDVAGQEIRNLE
                     PPIPAVPGYNVYLTIDTRLQAAAQATLEEEIRYWNTYFGQIRISSGVIIAINPKTGEI
                     LAMVSYPNYENNRFSRLIPAYYYDQLSQDPRRPLLNNAISAEYPPGSTFKLSTATGAF
                     NEGIVDLDTIVNAPGRLLLCERFNPNDPCTDNNTRPFVDHIFDQRPEGFGPITFT"
BASE COUNT          585 a          531 c          573 g          766 t
ORIGIN      
        1 caattgcaag cacaactcat tgaattgcaa caacgtgtaa atgagaccga aattcttgcg
       61 gctcttgttg atttttcccg ttcaaaccct gaaagtacat ataaagctgc atcggtcatt
      121 ggccgtgacc caagcccatt tttgcattac atcatcatca accgcggttc gaatgatgat
      181 attcggcgtg gtatgcccgt tgtgaccaat caaggtctcg tggggcgtgt tgatgcggtc
      241 attgcagatg ctgcccgcgt tcagttaatc acagatccat cttcttcggt caatgtatat
      301 ttgcaaaatg cagatacaag cgctgttttg tatggttcgg tcactggcga tgtttctctt
      361 gacctgatct ctcaaaatgc ctctgtagaa gcaggcgatt tgattctcac ctcagggttg
      421 ggtggaggat acccagcaga cctcctgatc ggacaagtcg tcacggttcg ctctttggaa
      481 tttgagctgt ttcaacaagc gacagttcaa cctgccgttg acttttctcg cttggaaatc
      541 gtccttgtca tcaccaactt ccgcccagtg gatattgctc cgcttgagcc cgtggttaca
      601 ccttccccgt aatttttcat gcgtaattta atcgcttttc cgttaatagg attggcagtc
      661 attctgcaat ctgcggttgt cagccaagta agtctactct cagggtatgc cgacttgccg
      721 ctcgtattgt tggcggcttg ggcactacaa gaccgtgtag attcttcttg gcattggggc
      781 gtcttaacct gtctctttgc cggattcatc tcaggtttac ctatttctgt tgtaattgct
      841 gggtacctcg gaattatatt gtttgcccgc attttacaaa gacgtgtatg gcaggcgcct
      901 ttgctggcaa tgttcagtgt ggttttccta gggacattgt tttttcatat tttgtctttt
      961 gttgtgcttc ggttttcagg tacacctttt gcttttggtg atgtgatagg attaatcacc
     1021 ttgcctagtt tattgttgaa catgttgatc tcgatcccaa tatatgcatt catgcgtgat
     1081 gtgtcttatt gggtgtatcc tgtaaaggag taagaatgag ttctagtgca gagtcccgtc
     1141 ctgttcggtt tgagggatgg cgattgggaa taatctatgt agttgtttct cttacattga
     1201 ttggatttgt gatccgttta gtgaatctac aaatattcaa tgttgaccag tacgaggcga
     1261 gagcggtgga aaattatgcc aaacctatta gtgttcctgc tccacgcgga attatttatg
     1321 accgtaatgg atacatcttg gctcgtaaca ttgctgcgta caatgtagta atcacgcctg
     1381 ccagtctacc tgctgaagac tcagatattc aacgcattta tcgtgaacta tctcaattga
     1441 ttgatgtacc tgtaggcaat tttgttgcaa atgaccctga aagtaacgaa atatttttat
     1501 ctgtccctgg cggtttgtta gcagagacat accttgaaga agcaaagttg tttgcttctt
     1561 gtgtgccggg accaagtatt ggtcagttag tggcattgca agatacgttg gctccatata
     1621 gtgcagtaaa gatcaaatgt aatgtttctg aagaaatagc tcgcatcgtg gaagagaaaa
     1681 agatggactg gcccggcgtg agcgtggaag tagagccgat tcgtgattat ccaactggct
     1741 cgctaacttc acatctgatt ggctttttag gtccaatccc cgcatcacaa gaagaagagt
     1801 tgaaggcatt gggcttcgac ccgaaccgcg ataaagttgg gtatgcaggc gtagagactt
     1861 cgttacaaga aatcttggcg ggcaggaatg gtttacgtgt tgtacaagta gatgtggcag
     1921 gtcaggaaat ccggaatttg gagccgccca ttccggctgt tccgggctat aacgtgtact
     1981 taaccattga tactcgttta caagcggctg ctcaagctac cttggaagaa gaaattcgct
     2041 attggaatac atacttcggt caaattcgta tttctagtgg tgtgatcatt gctataaacc
     2101 ctaaaacagg cgagattctt gccatggttt cgtatccgaa ttacgagaac aatcgtttta
     2161 gccgcttaat cccagcatac tattacgatc aattgagtca ggatccgcga agaccgttgc
     2221 tgaataatgc aatttcggct gaataccctc ctggttcgac cttcaagctt tcaacggcaa
     2281 ctggtgcttt taatgaaggt attgtcgatt tggataccat cgtcaatgct ccgggacgtc
     2341 tattactctg cgaacgattt aatcctaacg acccctgtac agataacaac acccgcccgt
     2401 ttgtagacca tatttttgac caacgtcctg agggctttgg tcctattacc tttac
//
LOCUS       DAOREF010000486         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31446_length_2452_cov_4.736754, whole genome shotgun sequence.
ACCESSION   DAOREF010000486 DAOREF010000000
VERSION     DAOREF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31446_length_2452_cov_4.736754"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..644)
                     /locus_tag="PKI33_10295"
     CDS             complement(267..644)
                     /locus_tag="PKI33_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37447.1"
                     /translation="MITKLFSDGVYGDADKSLRAAQVYYRQAQIKFPPIERPPFRETP
                     LRNNTSGYNGICETYCRTRKGEKISCWNVSWYNPPNKLNSKKFYFHDPQERKQALKEA
                     IQFRKEREAEILKRSRSRRKVIS"
     gene            complement(965..1306)
                     /locus_tag="PKI33_10300"
     CDS             complement(965..1306)
                     /locus_tag="PKI33_10300"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HNM37448.1"
                     /translation="MKTMGLKIIGIAVRTTNKDGQSARDIGELWGQFYSQNLLETIPN
                     KLSNDIYSIYTDYKSDFTDEYTTIIGLQVSSLDTIPSGLIGRQFPTETFEVFTAKGEM
                     PQAVMNIWLDI"
     gene            complement(1348..1803)
                     /locus_tag="PKI33_10305"
     CDS             complement(1348..1803)
                     /locus_tag="PKI33_10305"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNM37449.1"
                     /translation="MKTTTASPKGKSTTPTTSVKEFIASLDDEQSKADTHTLIEMMQR
                     ISGHAPKMWNVGTIGFDTYHYKYESGREGDSQTIGFYPRKGKLTVYLMDGTVRYSELL
                     SRLGKHTTSRVCIYIKRLSDIDLSILEQIVRQSYEYVKSQDGHMHRAFE"
     gene            complement(1856..2338)
                     /locus_tag="PKI33_10310"
     CDS             complement(1856..2338)
                     /locus_tag="PKI33_10310"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNM37450.1"
                     /translation="MIKFRAKIEIIGVNPFVFLPEHALTEIFKQANTTKGKIPIKMKI
                     DGHEFTQTLIKWQGAWRLYLNMPMRKAAQKDVGDTADFEIIYDPVKRGFPMHPKLKKA
                     LQENPSAKEVYESLRPSLQLEINRYLSFLKTEESVDRNVIRAIQFLLGKGRFIGRDKP
                     "
BASE COUNT          599 a          512 c          514 g          827 t
ORIGIN      
        1 cttggtcgta gactgaggct tagcgtccag ttgtttgtca ttcaaaacag agcacgcctg
       61 aataatctga aggtttgccc aattgttgtg ttctattaac ttcgtaagaa gactctcgtt
      121 ctgcaaggaa ttcattttct aagatttgct cctcacttat caattatttg taaataggtt
      181 atcttgtatt ttatttataa caatagccgt caaaacggca cgaaactttt ttgtgcatca
      241 ttttgacggc tacaaactca caacccttat gatatgactt ttcgtctact acgagagcgc
      301 tttaatatct cggcttctcg ttctttgcga aattggatcg cttctttcaa ggcttgcttg
      361 cgttcttgag gatcgtgaaa atagaacttt ttactgttca acttgtttgg cgggttatac
      421 caactgacat tccagcatga gattttctcg cctttgcggg tgcggcagta cgtctcacaa
      481 atgccattgt aaccagaggt attattccga agtggcgttt cccgaaaagg aggtctttca
      541 atgggtggaa attttatttg tgcctgccgg taatacactt gcgccgccct tagtgattta
      601 tcggcatctc cataaacgcc atcgctaaag agttttgtga tcactttagc attccgatac
      661 aacctgacta accagccagc cccctttgag tgtatcctgc tgatacccct caaagagtaa
      721 gtactcattc gattatcaaa ttcctttcgt atgtaagatg tgaaaggaat tatatctgac
      781 aaattttgta gaatatagag agcggttagc ggggttttga tcggttactt ggacagtatt
      841 ttgattattg ggtcctattg gttgctataa agatctgaac ttctgaattt tctccatttt
      901 gagatttctc tccatagaat tcaaagtcat aggtgtattt tctttgcaat tcattatctc
      961 gttgtcaaat atcgagccag atattcataa ccgcttgggg catttccccc ttggcagtaa
     1021 acacttcaaa tgtttcggta ggaaattgtc taccgatcag cccgctggga atggtatcca
     1081 aactgctgac ttgaagacct atgattgttg tatattcgtc tgtgaaatca cttttgtagt
     1141 cggtgtaaat agagtaaata tcgttactca acttgtttgg aatggtttca agtagatttt
     1201 gtgaatagaa ttgcccccat aactccccaa tatctcgagc ggactgacca tctttattgg
     1261 tcgttcgaac tgcaatacca attattttga gacccattgt tttcatcagg ttttctccgc
     1321 taccgatatg cgaaaagcat aggtttttta ctcgaacgcc cggtgcatgt gtccgtcttg
     1381 agactttacg tactcgtatg attgccgcac gatctgttca aggattgaca ggtctatgtc
     1441 gctaagccgt ttaatatata tacagacccg gctggttgta tgtttgccca aacgagacag
     1501 caactcagaa tagcggaccg tgccgtccat gaggtaaacg gttagtttgc cttttcttgg
     1561 ataaaagcca atggtctgac tgtcaccttc acgtccgctc tcatacttgt agtgataagt
     1621 atcaaaccca atcgtgccta cattccacat tttgggcgca tgcccgctga tgcgttgcat
     1681 catttcgata agagtatgag tatccgcttt tgattgttca tcgtccaacg aagcaatgaa
     1741 ctctttgaca gaagtggttg gggtagtgga cttgcctttt ggtgaagcgg tagttgtttt
     1801 cataagtcct aagattttag aacttatgtt ctatttcgtc aagattgaaa tttacttatg
     1861 gcttgtctct gccaataaat ctaccttttc ctaataggaa ctggatcgcc ctaataacgt
     1921 ttcggtccac ggactcttct gttttcagga agctcaaata gcggttgatt tccaattgca
     1981 atgagggacg taatgattcg tagacttctt ttgcggatgg gttttcttgc agcgcttttt
     2041 tgagtttagg atgcattggg aagccgcgct ttacagggtc ataaataatt tcaaagtcag
     2101 cagtatcgcc cacatccttt tgagccgctt ttcgcatcgg catgtttaga tacaaacgcc
     2161 aggctccctg ccattttata agggtctgcg tgaactcatg cccgtcaatt ttcattttga
     2221 tgggaatctt gcctttggtt gtgtttgcct gtttgaaaat ttctgtcaac gcgtgttcag
     2281 gaaggaacac aaaagggttt accccgatga tttcgatttt ggctctaaac ttgatcatgg
     2341 ttatttgact ttggctagta aggtttcaat agccgttata tgttccagct catgcaggga
     2401 cattgaacgt gcctgccaat agacggattc tttccgcttt tttcctttct ca
//
LOCUS       DAOREF010000487         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31519_length_2449_cov_2.691312, whole genome shotgun sequence.
ACCESSION   DAOREF010000487 DAOREF010000000
VERSION     DAOREF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31519_length_2449_cov_2.691312"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PKI33_10315"
     CDS             <1..1257
                     /locus_tag="PKI33_10315"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNM37451.1"
                     /translation="ACAKANLELQLLEPEKANAVIKACEDVRGGALHDQFVVDVIQGG
                     AGTSTNMNANEVIANRGLEILGHGRGEYKFLHPLEDVNMSQSTNDVYPTAVKVALRFL
                     INDLIDGMEVLRLAFAAKAEEFKDVLKMGRTQLQDAVPMTLGQEFSTYAVMLEEDQQR
                     LREGALLIQEMNLGATAIGTGINAHPEYAALARKYLSEITGIEYITAGNLVEATQDVG
                     SFVQLSGVLKRVAVKLSKICNDLRLLSSGPRTGLGEINLPAVQAGSSIMPGKVNPVIP
                     EVVNQIAFLVIGNDVTVSFAADGGQLQLNAFEPIIAHSLFDSLIYLRNGCITLAERCV
                     KGITANHNRLEEHMNRSVGIVTALNPFIGYENASSVAKEAYTSNKSVTEIVLERGLLT
                     KAELDEILKPEVLTKPRWIKKESGQK"
     gene            1260..1769
                     /locus_tag="PKI33_10320"
     CDS             1260..1769
                     /locus_tag="PKI33_10320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNM37452.1"
                     /translation="MDVLVRSETASDIEAISQVTYAAFLGKFSDHPTEHLIVNGLRNA
                     GMLTLSLVAEVDGNLVGHVAFSPVTIAEKFLDWYGLGPISVVPGLQKQGIGSKLILDG
                     LARLRDLGAKGCVLEGSPEYYSRFGFKPKVGLTYHAAPAPELFMALPFYDEVPTGQVE
                     YHKAFYISA"
     gene            1806..>2449
                     /locus_tag="PKI33_10325"
     CDS             1806..>2449
                     /locus_tag="PKI33_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNM37453.1"
                     /translation="MSTVFDSTKTVKNELGKQNYIASDEIATIVYLAQKLGKPLLAEG
                     PAGVGKTELAKAIASATGRELIRLQCYEGLDESKALYEWEYSKQLLYTQLLRDKLNDT
                     LGKAESLSEAADKLAKEEDVFFSTRFLLQRPLLKAILSETPTVLLIDEIDRADAEFEA
                     FLLEILSDFQVSVPELGTLAAKHKPVVVLTSNNTRELSEALKRRCLYLFVDYPS"
BASE COUNT          653 a          559 c          626 g          611 t
ORIGIN      
        1 gcgtgtgcaa aagcgaattt ggaattgcaa ttgctagagc ctgaaaaagc caatgccgtc
       61 attaaagctt gtgaagacgt gcgtggcgga gcactacatg accaattcgt ggttgatgtt
      121 atccaaggtg gcgccggtac ttctacgaac atgaatgcaa atgaagtgat tgctaatcgc
      181 gggttagaaa ttcttgggca tggacgtggg gaatataaat tcttgcatcc gcttgaagat
      241 gtgaatatga gtcaaagtac aaatgacgtt tacccaactg ctgtcaaagt agcgttacgg
      301 tttttgatca atgacttaat tgacggcatg gaagtattac gtctcgcatt tgcggcaaaa
      361 gccgaagaat tcaaagatgt tctaaaaatg ggacgcaccc agttacaaga cgcggttccc
      421 atgacgctcg ggcaggaatt cagtacctat gctgtgatgc ttgaagaaga ccaacagcgt
      481 cttcgtgaag gggcattgct cattcaagaa atgaacctcg gcgcgactgc cattggcacg
      541 ggcattaatg cacaccctga atatgctgca ctcgctcgca aatatctaag tgaaatcaca
      601 ggaattgaat acatcactgc tggaaatctc gtggaagcta cccaagatgt tggctcgttc
      661 gtacaactct caggtgtgct taagcgtgtg gcagtaaaac tctccaagat ctgcaatgac
      721 ttgcgattgc tctcctccgg tccgcggacg gggctgggcg aaatcaacct ccctgcagtg
      781 caagccggtt cgagcatcat gccgggcaag gtcaatccgg tgattccgga agtggtgaac
      841 cagattgctt ttctcgtcat cggcaatgac gtcactgtct ctttcgcagc cgatggcgga
      901 caattacaac tcaatgcctt cgagccgatt atcgcacaca gtctatttga tagtctcatt
      961 tacctgcgca atggctgtat tactttagct gagcgctgtg taaaagggat caccgccaac
     1021 cataaccgtc ttgaagaaca tatgaaccgc tcggttggaa tcgttacagc gttgaatccg
     1081 ttcatcgggt acgaaaatgc aagctcggtg gcgaaagagg cgtacacatc caacaagagc
     1141 gttaccgaaa tcgtactgga gcgcggtctg ctcacaaagg cagagcttga tgaaatcctc
     1201 aaaccggaag tgctcaccaa gccgcgttgg attaagaaag aatctggaca aaagtaagta
     1261 tggacgttct cgttcggtct gaaacagcaa gtgatattga agccatttca caagtcacgt
     1321 acgctgcgtt tcttggcaag ttcagcgacc atcccacgga acatctcata gtgaatggtt
     1381 tgcgtaatgc aggcatgctc accctttcac tcgtggctga agtggacgga aaccttgttg
     1441 ggcatgtagc gttctcacct gtgaccatcg ctgaaaagtt tttggattgg tatgggttgg
     1501 gtccgatctc agttgtgcct ggtttgcaaa aacaaggcat cggctcaaaa ttgattttgg
     1561 atggattggc aagactgcga gatctcggag cgaaaggatg cgtgctcgaa ggcagcccgg
     1621 aatattactc gcgcttcggg ttcaaaccga aagttggtct tacttatcat gccgcacctg
     1681 ccccggagct ttttatggca cttccttttt acgatgaagt tccaactggg caagtggagt
     1741 atcacaaagc gttttatata agtgcatgac gatttactac gtttcgctgt cgaataagga
     1801 gagagatgag tactgttttt gattcaacga aaaccgtaaa gaacgaactt ggcaaacaga
     1861 attacattgc ctctgatgaa attgcaacca tcgtatatct tgctcagaag ttaggcaagc
     1921 cattgctagc ggagggtccg gccggcgtag gcaagacgga gttggcaaaa gcgattgctt
     1981 cggcaacggg acgtgaactc atccgcttgc aatgctatga aggcttggac gagtccaaag
     2041 cgttgtatga gtgggaatat tccaagcaat tgttgtacac gcagttatta cgcgataaat
     2101 tgaacgatac attgggcaaa gccgaatcac tgagcgaagc cgccgataaa ctggcaaagg
     2161 aagaagatgt ctttttctcg acgcgctttt tgctgcaacg tccgctgttg aaagcgatct
     2221 tgagcgagac acccacagtt ctgttgatcg acgagatcga ccgcgctgac gcagagttcg
     2281 aagcctttct gctcgaaatc ttgagtgact tccaagtctc tgtgccagag ttaggcacac
     2341 tcgccgcaaa acacaaacct gttgtagtgc taacttccaa caacacccgc gaactctccg
     2401 aggcgttgaa gcgccgttgt ttgtatctgt tcgtggatta ccccagttt
//
LOCUS       DAOREF010000488         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31639_length_2444_cov_3.736291, whole genome shotgun sequence.
ACCESSION   DAOREF010000488 DAOREF010000000
VERSION     DAOREF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31639_length_2444_cov_3.736291"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PKI33_10330"
     CDS             <1..886
                     /locus_tag="PKI33_10330"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM37454.1"
                     /translation="KELGIPPGEENIEWWQTRLKVQFDQIWMIHDSYNIAGMQEVFEV
                     VRPVVERLGETDMLAEYYFLLPTLYFNSNGYQADEKIIEYSTLALELSLKGTDLELQS
                     RTQFGYGFVQLLIGNIDLAIKHLEEGLRLSTQIGYLDQQTYCITYLAVAHRRAGNLEE
                     CRQYSEQMLVISEREGQGSFTASAFANLGWLAWKQNDFAKAKTLSKKAAALWHPRYAF
                     HWHGLWTLIDISLRSHKVDEAVENVRRLRAPSQHVLSKELDDLMVDIIGQADKGNISN
                     SESLLKQAVAWVKEHHYL"
     gene            960..1361
                     /locus_tag="PKI33_10335"
     CDS             960..1361
                     /locus_tag="PKI33_10335"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNM37455.1"
                     /translation="MSVETTKEVLARYLNSGHSDQSMLAEDVSFTIMATGEEHRGREG
                     VSAMLNYFYHIAFEADAETRTLVFGETSAIWEGVIVGKHIGEYASVPATGKNVRVPIC
                     VVYDVEDKRVKRARVYFEVPAFYQQVGVKMG"
     gene            1584..1946
                     /locus_tag="PKI33_10340"
     CDS             1584..1946
                     /locus_tag="PKI33_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37456.1"
                     /translation="MKALRNTLIALAVYMTVVGALFLFAPSVAEKAFSTSLPDRALTP
                     LYGELMIVIALMAYLVSTDVSAYKKLVIAFIVSEIGHVLIFGYQLMTGIATFAQVGPP
                     MVIAIIFTVLLFVFKGKA"
     gene            1958..2176
                     /locus_tag="PKI33_10345"
     CDS             1958..2176
                     /locus_tag="PKI33_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37457.1"
                     /translation="MESDEAGARTKSLLFTLRLWSADENTHAPQWRVRLQNVHTGEVH
                     YCGDGKSLNTLLDDILSEVLKEKRKEER"
     gene            2185..>2444
                     /locus_tag="PKI33_10350"
     CDS             2185..>2444
                     /locus_tag="PKI33_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37458.1"
                     /translation="MLLLLLLILSLILSACGSSPAPTHAPTSGGFDIKPFLGVYEGTW
                     TNKTTGASGPAVIIVEAKEADKTVTLTLDFDGNYLGLNDPPA"
BASE COUNT          620 a          580 c          618 g          626 t
ORIGIN      
        1 aaaagaactt ggcattcctc ccggtgagga aaatattgaa tggtggcaga cccggctcaa
       61 ggtccagttt gaccagatat ggatgatcca tgattcatat aacatcgcag ggatgcagga
      121 agtctttgag gtcgttcgtc ctgtcgtgga acgtcttggc gagacggaca tgcttgcaga
      181 atattatttc ctgttgccta ctttgtattt caatagcaat ggctatcagg cagatgaaaa
      241 gatcattgaa tattccacgc ttgctttaga actaagcctc aaaggaaccg acctcgaatt
      301 gcagtcacgt actcaatttg gttatgggtt tgtgcaactg ctgataggaa atattgacct
      361 tgcaattaaa cacctggagg aaggtctgcg cctctcgacg cagatcggat acttggatca
      421 gcagacctat tgcattacgt atttggcggt tgcgcatcgg cgagctggaa atttggaaga
      481 atgccgccag tactctgagc aaatgctggt aatcagcgaa cgggaagggc agggcagttt
      541 taccgcttcc gcgtttgcca atttaggctg gctggcatgg aaacaaaatg actttgccaa
      601 agcaaaaaca ctttccaaaa aagcggctgc actctggcat ccgcgttatg ctttccactg
      661 gcatggcttg tggacgttga tcgatatcag cctgcgttct cacaaagtgg atgaggcggt
      721 tgagaatgtg cgtcggctga gggcaccgag tcagcatgtg ttatcgaaag agttggatga
      781 tctaatggtt gatattatcg gtcaggcgga taagggtaat atttccaaca gtgaatcttt
      841 gctaaagcaa gccgtggctt gggtaaagga acatcattac ctgtaacgtt acaatgaaac
      901 ggttttgaaa cggcttctct gtacactggg gacaactcaa caacttaagg agaagcacca
      961 tgtctgtcga aactacaaaa gaagtactgg cacgctacct caactctggt cacagtgacc
     1021 agagcatgct ggcagaagat gtcagcttta ccatcatggc aaccggtgag gaacaccgtg
     1081 gtcgagaagg tgtttcggcg atgctgaatt atttctatca catcgctttt gaagcagatg
     1141 ccgagacgcg caccctggtc ttcggcgaaa cgagcgccat ttgggaaggt gtcattgtgg
     1201 ggaagcacat tggcgaatat gcaagcgtac ctgccacagg aaagaatgtc cgagtgccga
     1261 tctgtgtggt gtatgacgtg gaggataaga gagtcaagcg ggctcgggtc tattttgaag
     1321 tccctgcttt ttatcagcag gtcggcgtca aaatgggata acctcccctc ttaatgcaaa
     1381 gtcgctaagc cgccaggttt caaattcctt tctttgcgac cctgcgcctt tgcgttaaaa
     1441 atatacaagg tgaaaaaagc tatgatcccc gccttctagg cggggatttt ctttcccgag
     1501 aaacgctcca tgaaacgctt ttgaaacgcc ccatccataa aatgagcaca gttcgatatt
     1561 tcaactcatt tacggaggct cttatgaaag cgttacgaaa tacccttatt gcattggctg
     1621 tctacatgac ggtcgtcgga gctttgttcc tctttgctcc cagcgtggcg gaaaaggcat
     1681 tcagtacatc cctgccagac cgcgccctca ccccgctcta cggcgaattg atgatcgtca
     1741 tcgccctgat ggcatatctc gtctccaccg atgtctccgc ctacaagaaa ttggtcattg
     1801 cattcatcgt ttcggaaatt gggcatgtgc tcatcttcgg ctaccagcta atgaccggca
     1861 ttgccacctt cgcccaggtc ggtccgccca tggtcattgc catcatcttc accgtcctgc
     1921 tctttgtctt caagggcaaa gcctaaaagg ttgtgatatg gaaagcgatg aggctggcgc
     1981 tcgcacaaaa tcgctcctgt ttaccttgcg tctttggagt gccgatgaga acacgcatgc
     2041 gccgcaatgg cgcgtgcgtc tccaaaatgt acacacgggc gaggttcatt attgtgggga
     2101 tgggaagtcg ctgaacacat tgctcgatga tattttgagc gaagttctca aggaaaaaag
     2161 aaaagaggaa cgatgattag aaaaatgtta ttacttcttt tgcttatttt gtctttgata
     2221 ttgtcagcct gcggttcttc ccccgctcca actcacgctc caacttcagg cggattcgac
     2281 atcaagccgt ttctcggcgt ctacgaaggc acttggacca acaagaccac tggcgcgagc
     2341 ggaccggcag tcatcatagt tgaagcgaaa gaagccgata agacagtgac cctcacgctt
     2401 gattttgacg gcaactatct tgggttgaac gacccgccag cctt
//
LOCUS       DAOREF010000489         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_31987_length_2429_cov_3.721567, whole genome shotgun sequence.
ACCESSION   DAOREF010000489 DAOREF010000000
VERSION     DAOREF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31987_length_2429_cov_3.721567"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKI33_10355"
     CDS             complement(<1..808)
                     /locus_tag="PKI33_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM37459.1"
                     /translation="MADTSLPFPTSDDMNPRLLEALESLNQISTAIKQIGISEDQSSS
                     LQLIAESAIRVVPGFSASIYIYDSATETLNAETRVVAYAEGYESSKHINQSEDIPRAN
                     GFGMRSIQRRRRAFSYEEADMQIHPYQQKLGVKAVAAFPLTVADDVLGVLYIYLYEDR
                     HLSQFEELMLHNFVNQAAMAIHHARNIAGIRQNLIQKDNELSRLRRAGLLISSRQKLE
                     ETLESILNLALELTNAQYGIFRLLDKSGEFLVTRAVSSLQLERPLVEKLPI"
     gene            complement(882..1511)
                     /locus_tag="PKI33_10360"
     CDS             complement(882..1511)
                     /locus_tag="PKI33_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNM37460.1"
                     /translation="MNPLLISSFILGIAFCAPPGIITTETIRRGAARGFFPALSVQFG
                     SLVGDTTWATIALTGLAFIIQNNIAKTILSVIGITLMIKLAWDAIKDAREGKHPADGT
                     AAESTRGDFASGAFLSLGNPMNIVFWTGLGTTVFASIAGTPQPIHFATFFTGFLAGAV
                     LWCFFIAGLVAWGRQWMTASFFRFLNAFCGIVLFYFALQLGLQTVQNFI"
     gene            complement(1639..>2429)
                     /locus_tag="PKI33_10365"
     CDS             complement(1639..>2429)
                     /locus_tag="PKI33_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNM37461.1"
                     /translation="HHHEVATAGQSEMSMTFTTLTRMADDLLLYKYIIKNMARKYGKT
                     VTFMPKPIFGDNGSGMHVHSSMWKDGKNVFYDEKGYAGLSQTAKYYIGGLLKHAPALL
                     ALTSPTTNSYRRLVPGYEAPVNIAYSQRNRSAICRIPANKSSKAKRVEFRAPDATANP
                     YLAFSAILMAGIDGIMNKIDPGEPKDVDLYELPAAEKALIKQVPSSLDAVLNALEADH
                     DFLLQGGVFTPDLLEEYIKYKRTFEVDAIRLRPTPHEYVLYFDA"
BASE COUNT          611 a          529 c          727 g          562 t
ORIGIN      
        1 tgatcggcaa cttctccaca agcggacgtt ccaattgcaa agaactgacc gcacgggtga
       61 cgaggaattc accagattta tccagtaggc ggaaaatgcc gtactgggca ttggtcaatt
      121 caagggctaa gttgaggata gactcgagcg tctcttccag cttctgacga gaagagatta
      181 acaaccctgc ccggcgcaaa cgagagagtt cgttatcttt ttggattagg ttttgtcgaa
      241 tccctgcaat attgcgagca tggtgaattg ccatggcggc ttgatttaca aagttatgta
      301 gcatcagttc ttcgaattgc gagaggtgac gatcttcgta taaatagata tacagcacac
      361 ctaagacatc atctgctacg gtgagcggga aggcggcaac ggctttgacc ccaagttttt
      421 gttgataggg gtgaatctgc atatccgctt cttcatatga gaaggcgcgg cgtctgcgct
      481 gaatggagcg catgccgaaa ccatttgcac gcggaatatc ttcactttgg ttgatatgtt
      541 tggaagattc gtaaccctct gcatacgcca ccacgcgcgt ttcggcgttg agcgtttcgg
      601 tggcagagtc gtagatataa atggatgcag aaaaacccgg caccacacga atggcgcttt
      661 ctgcaatcaa ttgcaagctg gaggattgat cttcgctgat accgatctgc ttaatggctg
      721 tgctgatctg gttcaagctt tcaagcgcct ctagcaggcg tggattcata tcatcggaag
      781 tggggaaggg taaagaagta tctgccatga gcaattgtta tacgataagg cgattgtact
      841 tcatggcgag ggcaggcaaa ccttgccctt acgtaatggg actaaatgaa attctgaacg
      901 gtttgtaatc ccagttgaag tgcaaagtaa aagaggacga ttccacaaaa ggcgttaagg
      961 aagcggaaaa acgaagccgt catccattga cgtccccaag caacgagacc tgcgataaag
     1021 aagcaccaca gaacggcgcc tgctaagaag ccagtgaaaa aggtagcaaa atgaatgggt
     1081 tggggtgtgc cagcaatgga tgcgaagacg gtcgtgccta gccctgtcca gaagacgatg
     1141 ttcattgggt tgccaagcga aagaaacgcg ccgctggcaa aatctccgcg tgtggattca
     1201 gcggcagtgc cgtctgcggg gtgtttccct tcgcgggcgt ctttgatggc atcccaagcg
     1261 agtttgatca ttaatgtgat tccgatcacg ctcagaatcg ttttggcaat attgttttga
     1321 atgatgaacg ccaatccagt taaagcgatc gttgcccagg tggtatcacc aaccaaggaa
     1381 ccaaactgca ctgaaagcgc ggggaaaaat ccgcgagcgg caccccgacg aatggtttct
     1441 gttgtgatga ttccaggagg agcgcagaag gcaattccga gaataaagga tgaaatgagg
     1501 agtgggttca tgtttataaa agatttgtag gggcgggtgc tgtgaatccc gcccctacct
     1561 aggaggaaca accgacaggt ggtcacctgc tcagggtttg caaccaactg tcggcggaga
     1621 gtgaagcgag gttagtgact aggcgtcgaa gtacaaaacg tattcgtgcg gggtcgggcg
     1681 gaggcgaata gcatccactt cgaaagtgcg cttgtacttg atgtattctt cgagcaagtc
     1741 aggggtgaac acaccaccct gaaggaggaa gtcgtgatcg gcttcaaggg cgttgagcac
     1801 ggcgtcgagg ctggaaggaa cctgcttgat gagcgccttt tcagcggcgg gcaattcgta
     1861 gaggtccaca tccttgggtt caccggggtc gatcttgttc atgatgccgt cgatacctgc
     1921 catgaggatg gcagagaaag cgaggtaggg gttggcggtg gcatcaggag cgcggaactc
     1981 aacacgcttg gctttggagc tcttgtttgc cggaatacgg cagatggcag agcggttgcg
     2041 ctgtgagtaa gcgatgttca cgggtgcttc ataaccgggc acgaggcggc ggtaggagtt
     2101 ggtggtggga gaagtgagtg cgaggagcgc aggagcgtgc ttgagcaaac caccgatgta
     2161 gtacttggcg gtctgggaaa gacccgcata acccttctca tcatagaaga cgttcttgcc
     2221 atccttccac atggaggagt gaacgtgcat accggagccg ttgtcaccga agatgggctt
     2281 gggcatgaag gttacggtct tgccgtattt acgagccatg ttcttgatga tgtacttgta
     2341 aagaaggagg tcatctgcca tgcgggtgag ggtagtgaag gtcatgctca tttcgctctg
     2401 accagcggtg gcaacttcgt ggtggtgca
//
LOCUS       DAOREF010000490         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_32090_length_2425_cov_3.786498, whole genome shotgun sequence.
ACCESSION   DAOREF010000490 DAOREF010000000
VERSION     DAOREF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32090_length_2425_cov_3.786498"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /gene="eno"
                     /locus_tag="PKI33_10370"
     CDS             complement(<1..551)
                     /gene="eno"
                     /locus_tag="PKI33_10370"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNM37462.1"
                     /translation="MDTTIESISALEVLDSRGNPTVEVEVLLMDGSWGRAAVPSGAST
                     GEHEALEMRDGDKKRYLGKGVANAVSNVNGVIAESLFGWDASDQRGIDAEMIAMDGTA
                     NKSKLGANAILGVSLAVAKAAANSFGMPLYRYLGGVYAHVLPVPMMNIMNGGAHTSWQ
                     STDMQEFMVMPFGAPTFSEGLRWG"
     gene            656..1351
                     /locus_tag="PKI33_10375"
     CDS             656..1351
                     /locus_tag="PKI33_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNM37463.1"
                     /translation="MRDEILTEVTFFDPRDKLHFAFRATLFRKVIIFSLGSLFKLIMD
                     LKVEGAENLPSDGAVVLVANHVTNFDVFPMQLSIPRPIFFMAKSGLFKFPILDVALRD
                     LGAFPVYRGEKDEWALRHAARVLEQGQTLGMFPEGTRSKGKGLGVAKTGSARLALDAN
                     CPIVPMAVIGTDKFFKRFPFRTKVIIKLLPPMLPKPGETPLALMDRVMFSLAKELPTE
                     MRGVYSEVPKGFE"
     gene            1364..1876
                     /locus_tag="PKI33_10380"
     CDS             1364..1876
                     /locus_tag="PKI33_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM37464.1"
                     /translation="MPENSQHGANIMPQEEHKHKEPIKDKLIPFLAYSKLNKTPVREF
                     AARLKAEGWIDPWFDEEDILPGQIWQNSVVTGVRQSHAVIIFLSKVAIASAGFYQKEI
                     KLALDTASEKPEGTIFIIPIRLNDCEVPEMLSKYQYIDYFGGLEQKEKVYSSLILSLK
                     LRAENLGIKV"
     gene            1946..>2425
                     /locus_tag="PKI33_10385"
     CDS             1946..>2425
                     /locus_tag="PKI33_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37465.1"
                     /translation="MKLTYKIFLLFLMSTLFLVACGGNDAAATPELSGTGAVETLVAS
                     FFATQTAMYTPPPTNTNTSTPTNTALPTPTIPSNTPPPLATWTPVIVYASSTANLALT
                     PSVTGTPPTATQNTGALGFGCNNLAIVRDVNYPSGTTVKAGDVITKTWKVQNTGSCDW
                     "
BASE COUNT          620 a          613 c          532 g          660 t
ORIGIN      
        1 ccccaacgca aaccttcaga gaaggtcggc gcaccaaacg gcatcaccat aaattcctgc
       61 atatcggtgc tttgccagct ggtgtgcgcg ccgccgttca tgatgttcat cataggcacc
      121 ggcaagacat gcgcatacac gccgccgaga tagcgataca gtggcattcc gaaggagttt
      181 gccgctgcct tggcaacagc caagctcaca ccgaggattg cattcgcgcc aagtttggac
      241 ttgttcgcag ttccatccat ggcgatcatt tcagcgtcga tgcccctctg gtcagatgca
      301 tcccacccga aaagggattc tgcgatcacg ccattgacgt ttgaaacagc attggctacg
      361 cccttgccga gatagcgttt cttatcgcca tcgcgcattt cgagtgcttc gtgctcgcca
      421 gttgaggcgc cagagggaac cgcagcacgt ccccatgagc cgtccatcaa taaaacttcc
      481 acttccacag tgggatttcc gcgggaatca agaacttcca acgcggagat gctttcgatt
      541 gttgtatcca tttgatttgc tcctaaatga aggcgacgat ttttgcttga tggtgttaat
      601 atcccaagac aggtcgcccc tgatttttat ttttcgccct agtataatcc caaatttgag
      661 agatgaaatc ttgacagaag tcaccttttt tgaccctcgc gataaactcc attttgcctt
      721 tcgagcgact ctatttcgaa aagtcatcat tttttcactt ggcagccttt ttaagctcat
      781 tatggacctc aaggtggagg gcgccgaaaa tctgccctca gatggtgccg ttgttttggt
      841 tgcgaatcac gtcacaaatt ttgatgtgtt ccccatgcag ttgagcattc cgcgccccat
      901 ctttttcatg gcgaaatctg gtttgttcaa attccccatc cttgatgttg ccctgcgtga
      961 cttaggcgct ttccccgttt atcgcggcga gaaagatgaa tgggcactgc ggcatgctgc
     1021 ccgtgtttta gaacaagggc agactttagg tatgttcccc gaagggacac gttccaaagg
     1081 aaagggactt ggcgtcgcca aaaccggctc tgcccgcctc gcgcttgacg caaactgtcc
     1141 catcgttccc atggctgtga tcggcaccga caagtttttc aaacgattcc cgttccgtac
     1201 taaagtaata atcaaactct tgccgcccat gttgccaaaa cctggtgaaa ccccacttgc
     1261 attgatggat cgagttatgt tttcgcttgc taaagaattg cccactgaaa tgcgcggagt
     1321 ctattccgag gtgccaaagg gatttgaata acgctgcgat ataatgcctg aaaactcaca
     1381 acacggagcc aacatcatgc cgcaagaaga acataaacac aaagaaccaa tcaaagacaa
     1441 actcattcca tttcttgcgt attccaagct caataaaacc cctgttcgtg aatttgcagc
     1501 ccgtctcaaa gccgagggct ggattgaccc ttggtttgac gaagaagata ttttgccggg
     1561 gcaaatttgg caaaattccg ttgtcaccgg ggtgagacag tcccatgctg ttatcatttt
     1621 cctttcaaag gttgcaattg cttcagctgg tttttaccaa aaagagatca agttagccct
     1681 tgataccgct tcagaaaagc cagagggtac gattttcatt attcctattc gcctaaatga
     1741 ctgcgaagtc cctgaaatgt tgagtaaata ccaatatatt gattatttcg gtggtttgga
     1801 acaaaaagaa aaagtgtatt ccagtttgat cctttcttta aaattgagag ctgaaaattt
     1861 ggggatcaag gtttaaatcc attctgtggt tctaaatccg aattgaagtt tcattttata
     1921 atcattacat agaaaggtta tctcaatgaa actcacatat aagatatttt tactgttttt
     1981 aatgtccaca ctttttttag ttgcctgtgg tgggaatgat gcggcggcta ccccagaact
     2041 ctctggaact ggagcagtgg aaacattagt ggctagcttc tttgctacac aaactgccat
     2101 gtacaccccg ccaccaacca atacgaatac ctcaactcca acaaacactg ccttaccaac
     2161 acccacgata ccgtcgaata ctccgcctcc gcttgccaca tggacgccgg ttattgtgta
     2221 tgccagttct actgccaacc ttgccttgac tccatccgtg acggggacgc ctcccaccgc
     2281 aactcaaaat acaggggctt tgggttttgg ctgtaataat ttagcgattg ttcgcgatgt
     2341 gaactatcca agcggtacaa cggttaaggc tggtgatgtt attaccaaaa cctggaaagt
     2401 tcagaatact ggctcatgtg attgg
//
LOCUS       DAOREF010000491         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_32302_length_2416_cov_4.692503, whole genome shotgun sequence.
ACCESSION   DAOREF010000491 DAOREF010000000
VERSION     DAOREF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32302_length_2416_cov_4.692503"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..342
                     /locus_tag="PKI33_10390"
     CDS             115..342
                     /locus_tag="PKI33_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37466.1"
                     /translation="MSVQTLENTMEKCETTTSDAAPAETPARSLFTRQTGQALITLGT
                     LFKHVPPFNESIDKSTGDTLISIGKKLKGKK"
     gene            415..1527
                     /locus_tag="PKI33_10395"
     CDS             415..1527
                     /locus_tag="PKI33_10395"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNM37467.1"
                     /translation="MAKKNNSSPKLVWDFGTAYELFISLHVLLEPEHFGIRPSYAASV
                     RARIPATERKLLEELYPLLGVPLKWLNTLPGPKDAISALWAIKQIPPAKRMIELYNVH
                     EVYDADKPEDVEKHKTFREILLRIAAEGKVLSTDVDFFQKLFSQKHGNMKREAVESAL
                     DWWSRPAELGEAFLTAFQSYYQAFFEEEEKRIEPILRAGLERAQALSSTVSFEELFTE
                     LSQGLQVGDEFNAPNYIIVPAFWITPLIFFERFDRNTMFLTFGARPADMSVIPGETVP
                     DALVRSLKALADPTRLKIMHYLTHESLTPSEIARKLQLRPPTVTHHLKELRLAGLVEL
                     SLMHEENRYTARKQALDAVYEHLNAFLQGENLQELA"
     gene            1524..>2416
                     /locus_tag="PKI33_10400"
     CDS             1524..>2416
                     /locus_tag="PKI33_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37468.1"
                     /translation="MNTTTSFKPFRILGEYSSHIRAFKPNARLYLLNVIITGAVMGVF
                     RLIFNFYALSLGFDESVLGNLITTSSFVALIAALPMGYLADIIGRKGSLILSSILLAF
                     SVIAMALWKTETSLYAMNVVSGLAQSLAGVTMSPFLLENSDEKERTYLFSFGQGLQMT
                     MASIGNWVGGYLPTWISQTQHVSETSSIAYGNSILIAGIGALIAVLPLMFIKSPNITR
                     SQRAVFAPFQYAAKNPGLLTKLVLPMLLTSLGAGLIMPFMNVFFRVVHHQPDPVIGTL
                     FAWGSLAMGIGLLIAPPLADRT"
BASE COUNT          615 a          706 c          508 g          587 t
ORIGIN      
        1 ctggaaattc gccctaccta cttgacaaac cgtctaattt agactattat ctaattagat
       61 atacgtctaa cacagtcatt tgcctaaccg tccccatcag agagagagga caccatgtcg
      121 gtacaaacct tagagaacac aatggaaaaa tgcgaaacca ccacatcaga cgccgctccg
      181 gctgaaaccc cagcccgaag cctcttcacc cgccaaaccg ggcaggcgct catcacgctt
      241 ggcaccctgt tcaaacatgt tcccccattc aatgaaagca tcgacaaaag caccggggat
      301 actctcatct ccatcggtaa aaaactaaaa ggaaaaaaat aaagcccaca cttcaggtga
      361 cagtccaatc gatgggctgt cacctgaact ttgaatacgg gcacactact atatatggct
      421 aaaaagaaca attcctctcc aaaactcgtt tgggattttg gcaccgccta cgagttattc
      481 atcagcttac atgttctgct tgaacccgaa catttcggca ttcgcccatc gtatgccgcc
      541 agtgttcgag cccgcattcc ggcaacagaa cgaaaactgt tagaggagtt gtatccgctt
      601 ttgggtgttc cgcttaaatg gctaaatacc ctgcccggac ccaaagatgc catcagcgca
      661 ctttgggcga ttaaacaaat tccgcccgcc aaacggatga tagaactgta taacgttcac
      721 gaagtgtacg atgccgataa accagaagat gttgaaaaac acaaaacctt tcgagagatt
      781 ctgttgcgca tcgcagctga aggcaaagtc ctttccaccg atgtagattt tttccaaaag
      841 ctcttctcgc aaaaacacgg caacatgaaa cgcgaagccg ttgaatccgc tttagattgg
      901 tggagcagac ccgccgaact cggtgaagcg ttcctcactg cttttcaatc ctactatcaa
      961 gcattttttg aggaggaaga aaagcgcatc gaacccatcc tgcgtgctgg gttagaaaga
     1021 gcccaagctt tatcttccac cgttagcttt gaagaattat ttactgaact ctcgcagggt
     1081 cttcaagtgg gtgatgaatt caatgccccc aactacatca tcgtccctgc attttggatc
     1141 accccgctaa tcttcttcga acgctttgac cgcaacacca tgttcctcac cttcggcgcc
     1201 cgccccgccg atatgtctgt catccccggc gaaaccgtac ccgatgcgtt ggtccgttcg
     1261 ctcaaagccc ttgcagaccc cacgcgcttg aagatcatgc attacctcac acacgagagc
     1321 ctgacccctt ctgaaatcgc ccgcaagttg cagctccgcc cacccacagt gacgcatcac
     1381 ctcaaagagc ttcgtctcgc cggtttggtg gaactctcgc ttatgcacga agagaatcgt
     1441 tacaccgccc gcaagcaagc gctcgacgca gtgtatgaac atctaaatgc cttcctgcaa
     1501 ggcgaaaatc tacaagaact cgcatgaaca caacaacctc tttcaaaccc ttccgtatct
     1561 tgggagaata tagctcccac atccgcgcat tcaaaccgaa tgcgcggctt tatcttctaa
     1621 acgtcatcat caccggtgca gtgatgggcg ttttccgcct catcttcaac ttctacgccc
     1681 tgagcctcgg cttcgatgaa tcggtgttgg gcaatctcat caccaccagc agtttcgtcg
     1741 ctctgatcgc cgctctgccc atgggctatc tggcagacat catcgggcgc aaaggttcgt
     1801 tgattctttc cagtatcttg ctggcatttt ctgtcattgc catggcatta tggaaaaccg
     1861 aaacatcgct ctatgccatg aacgtcgttt ctgggttagc acaaagttta gcaggcgtga
     1921 ccatgtctcc cttcctcctg gaaaatagcg acgagaaaga gcgcacttac ctcttcagct
     1981 ttggtcaggg cttgcaaatg accatggcat ctattggcaa ttgggttggc ggctacctcc
     2041 ccacttggat cagccaaacc caacacgtct ctgaaaccag cagcatcgcg tatggaaatt
     2101 ccattctcat cgccggaatc ggcgcattga ttgctgtgtt gccattgatg ttcattaagt
     2161 caccgaacat cacccgaagt caacgcgctg tttttgcacc ttttcaatac gcggcgaaga
     2221 accccgggct gcttacaaaa cttgtcctgc caatgttgct gacctcgtta ggtgcgggtc
     2281 tcatcatgcc attcatgaat gtcttcttcc gcgtggttca tcaccaaccc gacccagtca
     2341 ttggcacgct tttcgcttgg ggttcactgg caatgggcat tggtctgctt atcgcgccac
     2401 ctctcgcaga ccgaac
//
LOCUS       DAOREF010000492         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_32623_length_2404_cov_4.368668, whole genome shotgun sequence.
ACCESSION   DAOREF010000492 DAOREF010000000
VERSION     DAOREF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32623_length_2404_cov_4.368668"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1105
                     /locus_tag="PKI33_10405"
     CDS             71..1105
                     /locus_tag="PKI33_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37469.1"
                     /translation="MPRLKFLFALAALILSSLACVTLFGQKSASAGIAPGMAIPATAT
                     PEALSCPLITNKIVDQNATIDLSNQLPLDVLNSSVDDGDEAIYLVTYTVDGNEIRDPY
                     FEQDVPADLVDEQNDTALHQQLWEYFSTVIPLEARPTLAEFSIMTDGSDNVLAAVAQT
                     YDDPNRWSLEVDVADTSDYYYLTFTLVHEYAHLLTLGPDQVPPSLAVFNNPDDNDLYL
                     QEVAACATFFPGEGCSNPDSYMNAFYDQFWTNIYDEWNQINLEEDEDLYYEKLDDFYS
                     KYEDQFLTDYSATNPAEDIAESFGFFVFADKPDGDRIAEQKILFFYQYPELVELRTKI
                     LNNVCVNFPQ"
     gene            1109..1672
                     /locus_tag="PKI33_10410"
     CDS             1109..1672
                     /locus_tag="PKI33_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNM37470.1"
                     /translation="MKHLPIIESYWVEEGHFLAGEYPGSYNPEDARRRMDAFLESGIR
                     TFIDLTQPHELVPYEPILKEEARIYEHMAVYHRFAIRDHGIPSVQTMTLILDTIDAAI
                     ESGSPVYVHCWGGVGRTGTVVGCYMVRHGLTPSQALERVNALYKTRPQNYFLVRSPET
                     EEQADFVRKWRETPGTGHKAKQRFCEG"
     gene            1872..>2404
                     /gene="lepB"
                     /locus_tag="PKI33_10415"
     CDS             1872..>2404
                     /gene="lepB"
                     /locus_tag="PKI33_10415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNM37471.1"
                     /translation="MDTLEQEPLVEESKPAEETTNWKRFVLDVIETLVLAVILFVGIN
                     SVSARVRVDGFSMQPTLQDGEFVLVSKLSYKFGDFQRGDIIVFDFPMNPDEELVKRII
                     GVPGDHVMVRDSHVYVNEQMLNENYIAQAPLYSGDWIVPDGTLFVLGDNRNNSNDSKD
                     WGLLPQGNVVGKAVLIY"
BASE COUNT          601 a          523 c          626 g          654 t
ORIGIN      
        1 cgtaatgaga ctgttaattc gataaaaatt ccttatggta tccttggaaa aaaattccaa
       61 ggatattttt atgccccgtc tgaaatttct ctttgcgctc gctgcgctga ttctttcttc
      121 tttagcttgt gttacattat ttggacaaaa atccgcctcg gctgggatcg cgcctgggat
      181 ggcgataccg gctacggcta cgcctgaagc gctttcctgt ccattgatca ccaacaagat
      241 cgtggaccaa aatgcaacca tagacctttc caatcagctt cctttggatg ttttaaattc
      301 atctgttgat gatggggatg aggcgatata tttggtcaca tacacagtgg acggaaatga
      361 aatccgcgac ccgtattttg agcaggatgt gcctgcggat cttgtggatg agcaaaatga
      421 cacagcattg caccaacaat tgtgggaata tttttctaca gtgattccgc tggaggctcg
      481 ccctaccttg gcagaatttt ccatcatgac cgatggctcg gataatgtct tggcagccgt
      541 cgcgcagacc tacgatgacc cgaaccgttg gtctttagaa gtggatgttg ctgacaccag
      601 cgattattac tacctaacct ttactcttgt gcatgaatat gcccatttgc tcaccttagg
      661 tccagaccag gttccgccca gccttgctgt gttcaataac ccagatgata acgacctgta
      721 tttgcaggag gtggcggctt gtgccacatt cttccctgga gaaggttgct cgaaccccga
      781 ttcgtacatg aacgcttttt atgaccaatt ctggaccaat atttacgatg agtggaatca
      841 gataaacctc gaagaagacg aagatcttta ttacgaaaaa cttgatgatt tctacagcaa
      901 atacgaagac cagttcctga cagattactc tgcgaccaac cccgctgagg atattgcgga
      961 gtcctttggt ttctttgttt tcgcagacaa gccagatgga gacagaattg cagaacaaaa
     1021 gattttgttc ttctaccaat accctgaact agtggaactg cgcacaaaaa ttctcaataa
     1081 cgtgtgtgtg aatttcccac aatagcgtat gaaacatctt cccatcatcg aatcctattg
     1141 ggtggaagaa ggtcattttc tagcgggcga atacccgggc agttacaacc ctgaagatgc
     1201 ccgccgccga atggatgctt tccttgaaag cggtattcgc acatttattg atctgaccca
     1261 accgcatgag ttggttccat acgaaccgat ccttaaagag gaagcgcgta tctatgaaca
     1321 tatggcggtt tatcaccgct tcgccattcg cgaccatggc atcccttcgg tgcagacgat
     1381 gaccttgatt ctcgatacca tcgatgctgc gattgaaagc ggaagcccgg tgtatgtcca
     1441 ctgttggggt ggggtagggc ggaccggcac cgtggtggga tgttatatgg tgcggcatgg
     1501 gttgactccc tcgcaagcct tagagcgggt aaatgctttg tataagaccc gcccgcaaaa
     1561 ttactttttg gtgcgctcgc ccgaaacaga ggaacaggca gattttgtcc gcaagtggcg
     1621 agagacgccg ggaactgggc ataaagcaaa gcagcggttt tgtgagggtt aagactccat
     1681 tgcaggtgca agttttgctt taaggtggtg ctacgattgg cggatcaaag ctagagtgtg
     1741 gtaaaagccg aatggcagct gcccacttta catctcgcat gaagcggggt tacatcccca
     1801 ataagtaaga gttaagcatg gggaggctct ctggaaatgg cagtataatt tccgggcagt
     1861 ttggaggcat tttggatact ttggaacaag aaccgttggt agaggaatcg aaaccagcgg
     1921 aagaaacaac gaattggaag cgcttcgttc tggatgtgat tgagaccttg gtgctggcag
     1981 tgatcttatt tgtgggcatc aattctgttt cggcacgtgt gcgggtggat ggctttagta
     2041 tgcaacccac gttgcaagat ggtgagttcg tgttggtgag caagttgagc tataagttcg
     2101 gtgattttca acgcggcgat atcattgtgt tcgacttccc aatgaacccg gatgaagaat
     2161 tggtgaagcg tattattggg gtgcccggtg atcatgtgat ggtgcgcgat agccatgtat
     2221 atgtgaacga gcagatgttg aacgagaact atattgctca agcgccgctc tattctggcg
     2281 attggatcgt gccggatgga accttgttcg tgttgggtga taatcgtaat aactcgaatg
     2341 actcaaaaga ctggggcttg ctgcctcaag gcaatgtggt gggtaaggct gttttgatct
     2401 attg
//
LOCUS       DAOREF010000493         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33007_length_2387_cov_6.287307, whole genome shotgun sequence.
ACCESSION   DAOREF010000493 DAOREF010000000
VERSION     DAOREF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33007_length_2387_cov_6.287307"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKI33_10420"
     CDS             complement(<1..514)
                     /locus_tag="PKI33_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM37472.1"
                     /translation="MSKKKTSSELEKELLDAKQRIAELEQNEARFATILENSPVAIGI
                     SRLSDLKLIYVNSAFADLFGYSQDEILKNSTVDLGIWKDLEDRQKFFEIVRNKNKVTG
                     FEMSVRPQQGSEKNILVKGEMVEIDKQPCFVSQVIDITELKQSEAALQASEANYRNLV
                     ESSESAIAVVD"
     gene            747..2261
                     /locus_tag="PKI33_10425"
     CDS             747..2261
                     /locus_tag="PKI33_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37473.1"
                     /translation="MKILLATKLTAKFLLPIFASAILILTQAGKGILNLTDAPAPEIK
                     DSSSSFDTLRSATQPAQPTQASVIADTISYQIEAPVLPAYPFANPAGCSAAQPNLTPE
                     QMLTNVQNAIGNEYNLAERRQLYVWNGEQGSMLNVTNDPFVYEFTFNEFQHPLTYRGD
                     KVATIFMTQGFVVWFREYSDSFRLTAIPMTNGVMQSMWAPYVTSYWQQNGQPNDEKIY
                     PVMKKLPCHWVIDSGYVTNETLTTMFNLDWHTPDYLSAGRQYLASTCKEAYQISQEKV
                     GYWDATSMCGPLAWTIMRDVNAFPYRIGSWSQNAATFTRVNPRWDGQPWGSFDPETFT
                     LFHTEEAIAGYDFQSKGNLYTGDVVYTFATLYVTAGYFDHIFVVAGVDENNTRLAISN
                     MVQNSPYADCSIEEVKLYTPGDRETGVLNHEWNGNGYGKTGTTGFDVFRWNWITYHNN
                     GQAMSYTVRWGETIETIAFDWKISPESLQAANQFSADTQLTPGQIIILPAPTQS"
BASE COUNT          663 a          651 c          453 g          620 t
ORIGIN      
        1 tatctacaac tgcaatggca ctttcagagc tctcaaccaa attacggtaa tttgcctcac
       61 tggcttgcaa cgcagcttcc gactgcttca actcagtaat atcaatgacc tgtgatacaa
      121 aacaaggttg tttatcgatc tcgaccatct cccctttaac aaggatattt ttttcactac
      181 cctgctgtgg gcgaacgctc atctcgaaac ccgtcacttt attcttgttg cgtactattt
      241 caaaaaactt ctgccgatct tcaagatcct tccatattcc caaatctacc gtagagtttt
      301 tcagtatttc atcttgtgaa tatccaaaca gatctgcaaa cgccgaattc acatagataa
      361 gtttcagatc actcagccgt gaaattccaa ttgcaactgg gctattctct aaaatagtag
      421 caaaacgcgc ttcattttgt tccagctctg caatgcgctg tttagcatct aataattctt
      481 tttccagttc agaactggtc ttctttttgc tcataattta cttcctataa accctgatag
      541 aaacactctt aatatatttt tgcatagtat cgaaaacgag agaatggggg aattccctac
      601 ctgtaagttg cgtaaatcaa ccatctcatt ggcaggaatt tacccacgca aaattaggca
      661 aaaattacta aagaaatact agagaacacg tctgaggaaa cccagcctta aatgaaataa
      721 tgccgccaac acaaaaggtt gatggcatga aaattcttct cgctacaaaa cttaccgcaa
      781 aattccttct ccccatattt gccagcgcaa tccttattct cacccaagct ggcaagggga
      841 ttctcaactt aaccgacgcc cccgcccctg aaattaaaga ctcttcctca agtttcgaca
      901 cgcttcgttc cgcgacccag cctgctcaac ctacccaagc ttccgtcatc gccgatacga
      961 tttcctacca aatcgaagcc ccggtgcttc ctgcttatcc tttcgccaac cctgccggtt
     1021 gctccgctgc ccagccaaac ctcactcctg aacaaatgct taccaacgta caaaatgcca
     1081 tcggcaatga gtacaacctc gccgaacgcc gtcagctgta tgtatggaac ggggaacaag
     1141 gcagcatgct aaacgtcacg aatgacccgt ttgtttatga attcaccttc aatgaatttc
     1201 agcacccgct cacctatcgc ggagacaaag tcgccaccat cttcatgacc caaggcttcg
     1261 tcgtttggtt ccgcgaatat agcgatagtt ttcgcctcac cgcaatccca atgaccaatg
     1321 gcgtcatgca atctatgtgg gcgccttatg tcacttcgta ttggcagcaa aacgggcaac
     1381 ccaacgacga aaaaatctac ccggtcatga aaaaacttcc atgccattgg gtgatcgact
     1441 ctggttatgt gaccaatgaa actctcacca ccatgttcaa cctagattgg cacactcccg
     1501 attatctttc ggcgggtcgt caatatttag caagcacttg caaagaagcg taccaaatct
     1561 ctcaggaaaa agttggctac tgggacgcca cctcgatgtg cggaccttta gcatggacaa
     1621 tcatgcgtga tgtaaacgcc ttcccctatc gcatcggaag ttggtcacag aatgccgcga
     1681 cattcacccg tgtgaacccc cgttgggacg gacaaccctg gggatcgttc gacccggaga
     1741 cctttactct cttccatacc gaagaagcca ttgccggtta cgattttcaa tccaaaggaa
     1801 atctctacac aggcgatgtg gtttatacct ttgccacact atatgtcacc gccggatact
     1861 ttgaccatat ttttgtcgtc gctggtgtgg acgagaacaa tacccgcttg gcaatctcga
     1921 acatggtgca gaactcaccg tatgcagatt gctcgattga agaagtaaaa ctctacaccc
     1981 ccggcgaccg cgagaccggc gttctcaacc atgaatggaa tggaaacgga tacggcaaaa
     2041 ctggaaccac aggctttgat gtttttcgct ggaactggat cacctaccac aacaatggac
     2101 aagcaatgag ctataccgtt cgctggggcg aaacgattga aaccattgcg tttgattgga
     2161 aaatctcgcc agaaagcttg caagctgcca atcagttctc ggcagataca caacttactc
     2221 ctggtcagat tattattttg cccgcgccca ctcaatcata aaaattaatt ttgggcccct
     2281 cctgggcaag catgcaagaa gagaacagcc accatatggt ggctgttctc ttcttaatga
     2341 agcaaagctt ctaattcacg ttcatatttt tcccgcatcc gaatgga
//
LOCUS       DAOREF010000494         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33020_length_2387_cov_2.758576, whole genome shotgun sequence.
ACCESSION   DAOREF010000494 DAOREF010000000
VERSION     DAOREF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33020_length_2387_cov_2.758576"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKI33_10430"
     CDS             complement(<1..277)
                     /locus_tag="PKI33_10430"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNM37474.1"
                     /translation="MPRKKTTLPPQPPQEEDSVWTYRGYKLKSSEFVTAMVHLFRAEV
                     QRANVWRQRLDTTTNWAVVATGATLSISFSQPNVHHGVIILNTLLVTW"
     gene            complement(283..2082)
                     /locus_tag="PKI33_10435"
     CDS             complement(283..2082)
                     /locus_tag="PKI33_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNM37475.1"
                     /translation="MSQTSLTLLLLGVAAILLLRNILRADIVALMLMLALGLSGILTP
                     QEAFSGFSRQAVITMLSAFILAEGLRRSGMSERIGLFIIKLFGAGERRLIFGVMTASA
                     VLSLFMNNIAAASLLLPSISGIAHKMKISPSKLLMPLAFGTILGGMATLLTTVNLVVS
                     GLLRDENLPAFSLTDFLPMGLTLTFVGILFMVLWGRKLLPNQSPAQRHEEENASVELI
                     KTYQLAERLVRAEVCKGGRMDGVTLEKSGLREKYHLNVIAIQRRRNTLPIEAKTIIKG
                     DDILLIVARPEDSVPQSLGEMLNILPSGQWEQDYLSTPNMKLIEAAIAPRSHLASQTL
                     SEIRFEQKFNSKVLAIWRHGRSIRTRLADLPLEFGDGLLMQGTEKALSLLQTEPGLIL
                     LAESAPSTRMTWRNWLTVLIMFATMVLAVVFSNNIAEIMLTGSVMMVLIGTMNMDQAY
                     RSIDWRSLFLVAGMLPLGVALNKTGASTLFANAILAALGNANHLVLLASFVLLTVVLT
                     QIINGAAAVTVIAPIAIATSLQVGMEPRSVAMAVALASSMAFMSPLGHSVNVMVMGAG
                     GYTFKDYARVGIPLTILLVIILLFVLPILMPIG"
BASE COUNT          701 a          598 c          513 g          575 t
ORIGIN      
        1 accatgtaac taatagtgta tttaaaataa taacgccgtg atgtacattg ggctggctaa
       61 aagaaatgga aagcgttgcg ccagtggcaa ccaccgccca attggtagtg gtatccaagc
      121 gctgacgcca tacatttgca cgttgcactt ctgcgcgaaa aagatgcacc atggcagtga
      181 caaattcact gcttttgagt ttgtatccgc ggtacgtcca gacagaatct tcttcttgtg
      241 gaggttgagg aggtagggtt gtcttttttc ttggcataaa gtttatccaa tcggcataag
      301 aatggggaga acaaacaaaa gaataatgac aagcaaaatg gtaagaggga tgcctaccct
      361 tgcgtaatct ttgaaggtat atccaccagc gcccattacc ataacattga cggaatgccc
      421 aagtggtgac atgaatgcca tggaagaagc caatgctacc gccatggcta cactgcgcgg
      481 ctccatcccg acctgcagac tggtggcaat tgcgatggga gcgatcacag tgacggcagc
      541 agcgccatta ataatctggg tcaacacaac cgtaagcaaa acaaaacttg ccagcaatac
      601 caggtgattt gcattcccaa gtgcagctag aatagcattt gcaaacagcg tggatgctcc
      661 tgttttattc aatgcgaccc ccaacggtaa catcccagcc accagaaaca agctacgcca
      721 atcaatggac cgataggctt ggtccatatt catggtacca atgagcacca tcatcactga
      781 acccgtcagc atgatctctg caatgttatt ggagaataca actgctagta ccattgtagc
      841 gaacataatc aagaccgtca gccaattgcg ccatgtcatg cgagtagatg gggcagactc
      901 tgccaacaag attaacccag gttctgtctg caacaaactc aaagcttttt ctgtgccctg
      961 catcaaaagc ccgtcaccaa attcaagcgg tagatccgcc agccgtgtac ggatcgaacg
     1021 tccgtgccgc caaatggcta acacctttga attgaacttc tgctcgaaac gaatttcaga
     1081 caacgtttgt gaagcaagat gagaacgcgg cgcgatggca gcttcaatta atttcatatt
     1141 gggtgtggaa aggtaatcct gctcccattg ccccgatggc aatatattca acatctcccc
     1201 cagtgactgt ggcacactat cttcagggcg agccacgatg agtaaaatat catcgccttt
     1261 tattatggtc tttgcctcaa tgggtaatgt gttgcgtctg cgctggatgg cgatgacatt
     1321 gaggtgatat ttttcccgca aaccgctttt ttccagtgtc acaccatcca tacgcccacc
     1381 tttgcatact tctgcacgta ccaaccgttc agcaagttga taggtcttga tcaactcaac
     1441 tgaagcattc tcctcttcat gccgctgagc gggcgattgg ttcggtagta attttcgccc
     1501 ccataaaacc atgaaaagga ttcctacaaa tgtcagcgtc agccccatag gcagaaaatc
     1561 tgtcaaagag aacgcaggta aattttcatc gcgcaacagt ccactcacca caaggttgac
     1621 cgttgtcaac aaggttgcca tacctcccaa gatcgttcca aatgccagtg gcatcaacaa
     1681 tttcgatgga gagattttca tcttgtgcgc tataccagat atcgaaggca gcaataaaga
     1741 tgctgcggca atattattca taaacagcga caacacagca gatgcggtca tcacaccaaa
     1801 gattaatcga cgctcgcccg cgccgaacaa cttaataatg aaaagtccaa tacgctcgga
     1861 cataccagag cggcgcaacc cctccgcaag gatgaacgcg gaaagcatgg taattacagc
     1921 ctgtcggctg aatcctgaga aggcttcctg cggagtcaaa attccgctca aacccaaggc
     1981 gagcatcaac atcaacgcaa caatgtcagc ccgtaaaata ttccttagca acagaattgc
     2041 tgcaacacca agtaacaata aggttaaact agtttgtgac atatgatttt ggacggtttg
     2101 atgcttaatt catgcaataa atgagtttac acccactaaa gaaaatactt cgtgggtctt
     2161 tcattacgat tgctgtcaaa atcatgagac acatttactt cccgatctag tcgctaacct
     2221 tagaataaaa cagcccagtg aaatcactgg gctgttttat tctaatctcg agaaatgggc
     2281 aagttatcgt acatccacgc gccagttcca ccgaccacat tgtaaatagt cttgaatcct
     2341 ttttgaccta gcaaagccgc cgcagattga ctacgactgc cgcttgc
//
LOCUS       DAOREF010000495         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33135_length_2382_cov_5.335625, whole genome shotgun sequence.
ACCESSION   DAOREF010000495 DAOREF010000000
VERSION     DAOREF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33135_length_2382_cov_5.335625"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..784)
                     /locus_tag="PKI33_10440"
     CDS             complement(62..784)
                     /locus_tag="PKI33_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37476.1"
                     /translation="MGASLITKSVPTKRAPDAGDSAHIPSSFTRLIIFLAGRLRRPRP
                     SAGNASRWALAQITERTCFLMKKYQFWCPKCRKGFHENEVEKREVDTMFGEGRWWFCQ
                     TCDSSVVLYANQDLDLHSIFLALGDRAMNSVWQVSGVDCFGEKAEELCLIDDNKQSVS
                     GNDLLPITAGITQTIDGYFRAFDKVSTSHWFYMRAWDGRGFYVETNDHKIKERLDIRF
                     QNVEDVAEAQPPYEGLFIPRGE"
     gene            complement(788..1381)
                     /locus_tag="PKI33_10445"
     CDS             complement(788..1381)
                     /locus_tag="PKI33_10445"
                     /inference="COORDINATES: protein motif:HMM:NF037740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37477.1"
                     /translation="MHQYQTDDNAKTNFLIFAVLLAVGLAYLFGKAISALQIQIPWYV
                     ESPSILGFFGAIYWLYDKYLWKTKWVQMIDWIKTPNISGKWDVEIRTSHDSFTEKTSG
                     KAIIRQSAFRISIALETKSSISSSIHAALMRTEKVSEYELTYNYINHPKADSIETMSI
                     HMGTTRVSLSDDGKQMDGEYYTGRDRQNFGRITLLRT"
     gene            complement(1386..2258)
                     /locus_tag="PKI33_10450"
     CDS             complement(1386..2258)
                     /locus_tag="PKI33_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNM37478.1"
                     /translation="MTISETQLETWSHQGGVDAAQNTHTAIRNALNDYSWKSGVSYDD
                     YLQGSYRNTTNIYGNSDVDLAVELTSTFYHNELTESQKSALGFSSATYSINNFREDII
                     SILRNRFGTAYVDTSGGKSIKVLPNNGRLKGDVVVSAKYRHYEGTRLVAEGITFWNTK
                     TGEQIVNYPKIHLENGVTKHANTSQWYKPTVRIFKNARERICKNKTWLLGKFPSYFIE
                     CLLYNVPNAKYGQSYQDTFCNVVNWINDALTADPSQFVCQNKMFYLFGSSSTQWNVND
                     AKTFISELTSLWNS"
BASE COUNT          648 a          481 c          473 g          780 t
ORIGIN      
        1 tgggatgtgc gcagaatccc cagcgtcagg tgcacgcttt gttgggcgct ttttgacttt
       61 tttattcgcc acgcggaata aatagccctt cataaggagg ctgtgcttct gcaacatctt
      121 caacattttg aaatcggatg tctaaacgtt ccttgatttt atggtcattt gtctcgacgt
      181 agaatcctct accatcccaa gcacgcatat aaaaccagtg agaagttgag actttgtcaa
      241 aggctctaaa ataaccatca attgtttgag tgatacctgc tgtaatggga agcaaatcgt
      301 tcccgctgac agactgtttg ttgtcatcta tcaaacataa ttcttctgcc ttttctccaa
      361 agcaatctac accgcttacc tgccacacag agttcattgc tcgatcacca agtgcaagga
      421 atatgctgtg taaatctagg tcttggtttg cgtataaaac aactgaagaa tcgcatgttt
      481 ggcaaaacca ccaacgtcct tcaccaaaca tggtgtcgac ttctcttttc tcaacttcat
      541 tttcatggaa tccttttctg catttcggac accagaattg atatttcttc ataagaaaac
      601 aagttctttc cgtaatctgc gccagcgccc aacggcttgc gttacctgcg ctggggcggg
      661 gacggcgaag ccgtccagcc agaaaaatga taaggcgtgt aaaactgctt gggatgtgcg
      721 cagaatcccc agcgtcaggt gcacgctttg ttggcacgct cttggttatt aaagatgcgc
      781 ccatttttca agttcgtaat aaggtaatgc gtccaaagtt ttgacggtct cttcctgtgt
      841 aatattctcc atccatttgc ttgccatcat ctgaaagtga aacgcgagtt gttcccatgt
      901 gtatactcat tgtttcaatt gagtctgctt ttggatggtt aatgtaatta taggtcaatt
      961 cgtattcgct aactttttct gttcgcatca aggctgcatg aatactactt gatatagatg
     1021 atttagtttc aagtgcaata gaaatcctga atgctgattg acgaatgatg gcttttcctg
     1081 aggtcttttc tgtaaatgaa tcatgagatg ttcgaatctc aacatcccat tttcctgaaa
     1141 tgttgggagt cttaatccaa tctatcattt gaacccattt tgttttccaa agatatttgt
     1201 cgtacaacca gtaaattgct ccaaaaaatc ctagaattga tggggattca acgtaccagg
     1261 gaatttgaat ttgtaaagca cttattgctt ttccaaacaa gtaggcaagc ccaactgcca
     1321 ataaaacagc aaatataagg aagtttgttt tagcgttgtc gtcagtttga tattggtgca
     1381 ttttgttatg aattccagag agaagtcaat tcagaaataa aagttttagc atcattgaca
     1441 ttccattgtg tggaactgga gccaaaaaga tagaacattt tgttttgaca tacaaattgc
     1501 gatgggtctg ctgtaagagc gtcgttaatc cagttaacta cattgcaaaa agtgtcttgg
     1561 taagattgac catacttcgc gtttggtaca ttgtaaagca aacattcgat aaaataagat
     1621 ggaaacttac ccaaaagcca agttttgttt ttacatatac gttctcgcgc gtttttgaaa
     1681 attcgtacag ttggcttgta ccattgtgac gtatttgcgt gtttagttac accattttct
     1741 aaatgaattt ttggatagtt gacaatttgt tctcccgttt ttgtattcca aaatgttatg
     1801 ccttcggcaa ctaaccttgt accttcgtag tgtctatatt ttgccgaaac tactacatca
     1861 cctttgaggc gtccattatt tggtaatacc tttatcgatt taccacctga tgtgtcaaca
     1921 tatgcagtcc caaaacggtt tcttagaatg gaaattatat cttcgcgaaa attattgatg
     1981 ctataagttg cggacgaaaa gcccagggcg ctcttttgag attcggtcaa ctcattgtgg
     2041 taaaaagtgc ttgttaattc aactgctaaa tcaacgtcgc tattgccata aatatttgta
     2101 gtattgcgat aagagccttg taaatagtca tcgtaagaga caccactttt ccaagaataa
     2161 tcattcaaag cattgcgaat tgcagtatga gtattttggg cggcatctac accaccttga
     2221 tgtgaccatg tttcaagttg ggtttcagag attgtcatgc ttattttccc ttcctttatt
     2281 ttattcactc aattcaaggt gcagatatat attttgcaac gtggattttt taccttgtta
     2341 tgcacggggc gtgccaacgg catgcgttac ctgcgctggg gc
//
LOCUS       DAOREF010000496         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33136_length_2382_cov_4.702192, whole genome shotgun sequence.
ACCESSION   DAOREF010000496 DAOREF010000000
VERSION     DAOREF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33136_length_2382_cov_4.702192"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PKI33_10455"
     CDS             complement(<1..975)
                     /locus_tag="PKI33_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37479.1"
                     /translation="MSFRKTVSVFLLAILIVSCAPATEEPATFPTYNPFVSTNGATLP
                     PNSNVAVTTPLSSETVIPSATSTRPPTPTRVPLNISSLIGVQSQIISGSPTPDTARVL
                     PTPRQDADQYVVQAGDTLGAIAQRYNITLQTLMDANGITDPNALEVGMTLNIPAAQVT
                     VSSGQSLKIIPDSELVNGPATAKFSLRNFIASQNGYLNYYTQDVNGTLLNGADIIEAV
                     SYNYSVNPRILLALLEYQSGWVTNPTPTNIDYPMGYYDEARAGLYRQVVWAANTLNRG
                     YYLWKANGLSTWVLTDGQVLPIDPTINAGTAAVQYFFSQLYGYDAWQYA"
     gene            complement(1053..1499)
                     /locus_tag="PKI33_10460"
     CDS             complement(1053..1499)
                     /locus_tag="PKI33_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNM37480.1"
                     /translation="MTTVRKLLEAKEFATNYSVETNQTVLDALKVMAEAHIGAVLVTD
                     GGKIVGIYTERDYLYKGEALGRVAKDTKIKDVMTPKMINVTMETTVEQCMGLMKQYKI
                     RHLPVVEGEHFVGLVSMRDVMLAAIDIKESEIRGLENYIMGSGFQS"
     gene            complement(1637..2143)
                     /locus_tag="PKI33_10465"
     CDS             complement(1637..2143)
                     /locus_tag="PKI33_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNM37481.1"
                     /translation="MKKILLLGYGNPDREDDGVAWHILRALAVRMGLPAPDSYEDDFP
                     ENSQVDFAFHLQLTPEMAEDIAQYEYVCFVDAHTGNIPEEVRIIDVESDFQKSPFTHH
                     LTAQSLMSICETVYHRKPDAALLSVRGYQFLFSRLLSTPTAALVPEAVDLAWAWINAR
                     EPLKQAGD"
     gene            complement(2201..>2382)
                     /locus_tag="PKI33_10470"
     CDS             complement(2201..>2382)
                     /locus_tag="PKI33_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNM37482.1"
                     /translation="LTECADLMYQHHVRHLPIMEKEKVVGIVSIRRLAEALLAEDKGT
                     INKLEHYILGTGYGE"
BASE COUNT          614 a          545 c          574 g          649 t
ORIGIN      
        1 agcgtattgc caggcatcgt aaccgtaaag ctgtgagaag aaatattgta cagcggctgt
       61 gccagcgttg attgtagggt caatgggaag cacctgacca tctgttaaca cccaagtaga
      121 aagcccattg gctttccaga gataataacc gcggtttagt gtgtttgccg cccaaaccac
      181 ctgccgatat aacccggcgc gggcttcgtc gtaatacccc atggggtaat cgatattggt
      241 tggtgttggg ttggtaaccc agccgctttg atattcaagc aaggcaagta aaatgcgcgg
      301 gttgacggaa tagttataag acacggcttc aataatatct gcgccgttta ataaagttcc
      361 attcacgtct tgggtgtagt aattcaggta gccattctgg ctggcaatga aattgcgtaa
      421 actgaatttt gccgtagccg gtccgttgac cagttcggag tctggaataa ttttgaggga
      481 ttgtccgctt gatacagtaa cttgagcggc tggaatgttt aatgtcatgc caacttctaa
      541 tgcatttggg tcggtgatgc cattggcatc cataagagtt tgaagggtga tgttataacg
      601 ttgagcgata gcacctaagg tatcaccagc ttggacgaca tattgatccg catcttggcg
      661 cggagtgggc aacacccgag ccgtatctgg agtaggtgag ccggagataa tctgactttg
      721 tacaccaatg agagaagaaa tgttcaaagg cacacgagta ggcgtgggcg ggcgcgtgga
      781 ggttgctgac ggaataaccg tttcagaaga cagaggcgtg gtcaccgcca cgttgctatt
      841 tggcggaagg gttgctccat ttgtagaaac gaaagggtta taggtaggga aagttgcagg
      901 ttcttctgtg gcaggtgcgc aagaaacgat gagaatagca agtaaaaaca cagatacagt
      961 tttgcgaaaa gacataaaag tgattttacc aaatcgtatc ggtaaaaaaa gaggcgcaaa
     1021 cattgcgcct cttttcaagt ttgttgagag aactaggact gaaaaccaga ccccatgatg
     1081 taattttcaa gcccgcgaat ttcgctttct ttgatgtcaa tcgcagccag catcacatca
     1141 cgcatagaaa ccaaacctac aaaatgttcg ccttccacta caggtaagtg gcggatctta
     1201 tactgtttca tcagccccat gcattgctca acagtagttt ccatggttac gttgatcatt
     1261 ttcggtgtca tcacatcttt gatctttgta tctttggcaa cccgccccaa cgcttcccct
     1321 ttgtagagat aatcacgttc agtataaatt ccaacgatct ttccgccatc tgttaccaac
     1381 acagctccaa tatgggcttc agccatcacc ttgagggcat ccaataccgt ctgattggtt
     1441 tcaacagaat agtttgtcgc aaattcctta gcttccagaa gttttcggac agtggtcatg
     1501 attttcgtct ccttatataa gttctttttt atcataggca aaataaaacg tcacacggaa
     1561 aacctcctca tctcgcgtga cgtttgtcat ctgatttatt tttgacccgc ctgcactctt
     1621 tgctttcggc tcatccttaa tctccagcct gtttcagcgg ttctcgggca tttatccacg
     1681 cccaagctaa gtccacagct tcgggcacaa gagcagctgt cggcgtggaa aggaggcgcg
     1741 agaacagaaa ttgatatccg cgcaccgaga gcaatgctgc atctggtttg cgatgataga
     1801 ctgtttcgca aatggacatc aacgactggg cggtgaggtg atgcgtgaat ggtgatttct
     1861 gaaagtcgct ttctacatca atgatgcgta cttcctctgg aatattgccc gtgtgcgcgt
     1921 ccacaaagca gacgtattca tattgagcaa tatcttccgc catttcgggc gtgagttgca
     1981 gatgaaaagc gaaatctacc tgtgagtttt cagggaaatc atcctcgtat gaatcgggtg
     2041 caggcagccc cattcggacc gccaacgccc gcaggatgtg ccaggcaaca ccatcatctt
     2101 ctcggtcggg gtttccgtag cctagtaata aaattttttt cattcaacct ctaaaacctt
     2161 gataaacctc cgaagtctct aagacctcgg aggtttagga ttactcgcca tatcccgttc
     2221 caaggatgta atgctcaagt ttattgattg tccctttgtc ttcagccagt aaagcttctg
     2281 ccagcctgcg aatggaaacg atgcctacga ctttttcttt ttccataatg ggcaggtgtc
     2341 gcacatggtg ctggtacatc aggtcggcgc attcggttaa ca
//
LOCUS       DAOREF010000497         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33163_length_2381_cov_3.296217, whole genome shotgun sequence.
ACCESSION   DAOREF010000497 DAOREF010000000
VERSION     DAOREF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33163_length_2381_cov_3.296217"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..559)
                     /locus_tag="PKI33_10475"
     CDS             complement(212..559)
                     /locus_tag="PKI33_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNM37483.1"
                     /translation="MFNYTSFDESKVEFVEIAPASELPNGERLFVDLGDKPVVIFNIA
                     GQFFAIGDVCTHDDGPLGDGMLENFNVVCPRHGAEFDIRTGQAVQMPAVVDIPAYPVQ
                     VRDGTIFIGLPKE"
     gene            complement(575..1015)
                     /locus_tag="PKI33_10480"
     CDS             complement(575..1015)
                     /locus_tag="PKI33_10480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM37484.1"
                     /translation="MASYQIREMVIDDYDAIIVMFHETPGITVRDADSRESTAAYLMR
                     NPGLSFVATINGKIIGCVMSGHDGRRGYLQHLIVTPEFRRQGIGKALYLACLAALQKI
                     GIYKTHLFVQKSNSSGEEFWREQGWVFRDEVLMFSFNASPNENI"
     gene            complement(1021..1416)
                     /locus_tag="PKI33_10485"
     CDS             complement(1021..1416)
                     /locus_tag="PKI33_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNM37485.1"
                     /translation="MDDLYREVIIEHYKNPSYRGKLDPHDISFADNNPLCGDHIQIDL
                     RVDANGVVSEARFDGHGCAISQASADLLMETIIGKPLEEVKKLNRQDILDMLGIDLGP
                     VRLKCALLSLKVLKAGVYGLGEAGDDLVE"
     gene            complement(1465..>2381)
                     /locus_tag="PKI33_10490"
     CDS             complement(1465..>2381)
                     /locus_tag="PKI33_10490"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNM37486.1"
                     /translation="SWARANLKAGDLVILTEMEHHSNLVPWHMLQAERGIELEFIPVT
                     EDGLLDLEAYKTLLNRTPKLVSFTHMSNVLGTINPAAEIIKLAHAAGAVTLVDGAQSV
                     PHLAVDVQALDADFYAFSAHKMCGPTGMGVLYGKSALLEAMPPFLGGGDMIKEVKLRS
                     FRPNTLPHKFEAGTPAIAEAIGLGAAVDYLTQIGMSDIAAHEHEITEYALERLEEIPG
                     VKLFGPSADKKGGVAAFTLEGVHPHDVAQILDQDGIAVRAGHHCAQPLHEKFGIPATS
                     RASFYLYSTKDEVDMLVKGLYKVKEMFG"
BASE COUNT          639 a          622 c          523 g          597 t
ORIGIN      
        1 tttgaccaag acggtcagat tgtccgcgta tggggcgact atagcaatgc aaacaccggc
       61 tttgggttgg catcaggtgt tgctgttgac cctgatgggc acgtatgggt tagtgactcg
      121 gcgttcaacc gcgtcatgcg atttacactc ccgtaaactt tttataaaaa agcccctcaa
      181 tttatttgag gggctttttt attaggtaaa cttattcctt cggcaagcca ataaatattg
      241 ttccatcgcg gacttgaacc gggtaggcag ggatatccac caccgcaggc atttgaacgg
      301 cttgcccggt gcgaatgtca aactcggcgc cgtgtcgcgg acagaccaca ttaaagttct
      361 caagcatccc atctcctaac ggaccatcat catgggtgca gacgtcacca attgcaaaga
      421 attgacccgc gatattgaat atcacaacag gcttatcgcc taaatcaaca aagaggcgtt
      481 ccccattcgg gagttcggaa gcaggggcaa tttctacaaa ttcaactttt gattcatcaa
      541 aggaggtgta attaaacatg gttgttctca ggtttcatat attttcgttt ggagatgcat
      601 tgaaagaaaa catcagcact tcatctcgaa agacccagcc ctgttctctc caaaactctt
      661 ctccagaaga attcgacttt tgtacaaaaa gatgggtctt atagataccg atcttctgaa
      721 gggcagcaag gcaagctaaa tacaaagcct tgccaatgcc ttgtcttcga aattcaggcg
      781 ttacgatcag gtgttgcaaa tagccgcgcc gcccgtcatg cccagacatt acacagccga
      841 taatctttcc atttatggtg gctacgaagc tcagcccggg gtttctcatc aaataagcgg
      901 ctgttgattc tcgcgagtct gcgtctcgta ccgtgatgcc cggcgtctca tgaaacatca
      961 caatgatggc atcgtaatca tcaatgacca tctcgcggat ctgatatgaa gccacgcttc
     1021 ttactccacc aaatcgtctc cggcttcccc taagccatat actccagcct tcaacacttt
     1081 aagcgaaagc aatgcacact tcaaacgcac cggtccgagg tcaataccga gcatatcaag
     1141 aatatcctgc cggttgagct tttttacttc ttccagcggc ttcccgataa tagtctccat
     1201 taacaggtct gctgaggctt gagagatcgc acaaccatgc ccatcaaacc gcgcctcgct
     1261 aacaacacca ttcgcatcca ctcgcaaatc gatttggata tggtcgccgc ataatgggtt
     1321 gttatctgca aaggaaatat cgtgcgggtc taactttccg cgataggatg gatttttata
     1381 gtgctcaatg attacttcac gatagagatc gtccattata tattccgttt cagggaaaca
     1441 gggcaaggaa acctcgccct ataattaccc gaacatttct ttcactttat ataaaccctt
     1501 taccaacata tcgacttcat cttttgtaga gtacaaatag aaagaagctc ggctggtagc
     1561 agggatgcca aacttttcat gcaaaggctg agcacagtga tgccccgcgc ggacggcgat
     1621 gccatcctga tctaaaattt gcgccacatc atgggggtgc acaccttcaa gtgtaaaagc
     1681 agctacgcca ccttttttat ccgcagaggg accaaacagc tttacaccag ggatttcttc
     1741 caagcgttca agcgcatatt cagtgatctc atgctcatgg gcggcaatgt cgctcatacc
     1801 gatctgggtc aaataatcaa cagcagcacc taaaccgatg gcttctgcga tggcaggtgt
     1861 acccgcttca aatttatgcg gcaaagtgtt cgggcggaag gaacgcagtt ttacttcctt
     1921 gatcatgtca ccaccaccaa ggaacggagg catggcttct aacaaagctg acttgccata
     1981 caacacaccc atcccggtgg gaccacacat cttatgcgca gagaacgcat aaaaatcagc
     2041 atccaaggct tgaacatcca cggctaagtg agggactgat tgagcgccat ccactagagt
     2101 gacagcaccc gccgcatgag ctaatttgat gatctctgcc gccgggttga tcgtgcccaa
     2161 cacgttggac atgtgtgtaa acgagaccag tttcggggtc cggttgagaa gcgttttata
     2221 agcttccaga tccagcaaac cgtcttccgt cacagggatg aattccagct caatcccacg
     2281 ctctgcttgc agcatatgcc acggaaccag attgctatgg tgttccatct cagtcagaat
     2341 aacaaggtcg cccgccttta aatttgcacg cgcccaagaa t
//
LOCUS       DAOREF010000498         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33272_length_2376_cov_5.523481, whole genome shotgun sequence.
ACCESSION   DAOREF010000498 DAOREF010000000
VERSION     DAOREF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33272_length_2376_cov_5.523481"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PKI33_10495"
     CDS             <1..1005
                     /locus_tag="PKI33_10495"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNM37487.1"
                     /translation="VIQVGITLDGNWLTGENALDAFEKGDTSDLDRVIPPTDPASRQS
                     LHVLRDTKMGEKFETLAAIDVFFPVLHGPFGEDGTIQGLFEMADVAYVGAGVAGSSVG
                     MDKGIFKDVMRANGIPQVPSMLVLRGDIESKMDDVITRAEELGNYPLFTKPANLGSSV
                     GISKCNNRSDLAEGLMDAARYDRRIVIESGVKEVREVEVSVLGNEEPQASVCGEILPS
                     REFYSYESKYIDGTSGLLIPSPLPEEISNKIREYAIRAYKAIDCAGMARADFFVEKET
                     NKIYLNELNTLPGFTSISMYPKLWQASGLTYAQLVDRLIELALDRKAQRDRTEHRRTA
                     "
     gene            1002..2069
                     /locus_tag="PKI33_10500"
     CDS             1002..2069
                     /locus_tag="PKI33_10500"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNM37488.1"
                     /translation="MSEKRPLTRAEQVRQRRTERAAKELQQAAKQATKPIVKVSSRTP
                     TIPIMAAPKKQQDRRYNIALGMPEFHLKKPKFSWPQFSAPRMPRMPQFSANWRLGALI
                     LSVVFVVALVLAFQLPYFYVPQAFVSGNARIPSEEINGIMGVSNQNIFTVQPDELERR
                     LRLNYPEITSAQVDVYLPNYVYVTVTERQPVVMWRQNGGFTWIDATGVAFRPRGEAAA
                     LAVVDAVDAPPAGVQTSADPYSPPPFIQKEYVDAALALAPLVPAGSTLTYSAADGFSW
                     KDPRGWTVAFGTTANDVPLKIRVYQSLVDSLTQKGKIPEFISVVYPDGPYYRMKKAVT
                     VEEDQTAQTDQSTEETVVENP"
     gene            2071..>2376
                     /locus_tag="PKI33_10505"
     CDS             2071..>2376
                     /locus_tag="PKI33_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNM37489.1"
                     /translation="MEEIVVGIDVGTTKICTLVGRVEDDKSIRILGVGIEPSEGIRKG
                     IVVDLAAASRAIKRSVEKAESTSSLEITTAFVSLAGAHVSSINSRGSAGIPSGVIDIH
                     "
BASE COUNT          572 a          600 c          611 g          593 t
ORIGIN      
        1 gtgattcaag taggcattac tctggatggc aattggttga ccggtgaaaa tgcattagat
       61 gcttttgaaa agggcgatac cagcgattta gatcgagtca tccctcctac ggacccggct
      121 tcacgccaga gtttacatgt cttgcgcgat acaaaaatgg gcgagaagtt tgaaactctt
      181 gccgcgattg atgtgttctt ccccgttctg cacggaccct tcggcgagga tggcaccatt
      241 caagggctgt tcgaaatggc agatgttgct tatgtgggcg caggggttgc gggctcttct
      301 gtaggcatgg ataaaggcat cttcaaagat gtgatgcgtg ccaacggtat tccgcaggtg
      361 ccatctatgt tggtgttgcg cggtgatatc gaaagcaaga tggatgatgt catcacaaga
      421 gccgaagaat tgggcaatta tcccttgttt accaagcctg ccaatctcgg ttcctctgta
      481 ggtatcagca aatgcaataa ccgttcagac ctcgctgaag gtttgatgga cgcagctcgt
      541 tacgatcgcc gtattgtgat tgaaagcggt gtgaaagaag tgcgcgaggt tgaagtgagt
      601 gtgttgggca atgaagaacc ccaagcttct gtttgtggtg agatccttcc gagccgcgag
      661 ttttattcgt atgaatcgaa atacattgac ggaacctctg gcttgttaat cccttcgccg
      721 ttacctgaag aaatttccaa caagattcgt gagtatgcca ttcgtgccta caaagctatt
      781 gattgtgcag gcatggcacg cgccgatttc tttgttgaaa aagaaaccaa taaaatttac
      841 ctaaacgaat tgaacacgct tcctggtttt acttccatta gtatgtaccc caagctttgg
      901 caggcgagcg gcttgaccta cgctcaactg gtcgaccgcc tgatcgagct tgcgcttgac
      961 cgcaaagcac agcgtgatcg taccgaacac cggaggacag catgagcgaa aaacgacccc
     1021 tcactcgtgc ggaacaagtc cgccagcgcc gcaccgaacg cgctgcaaaa gaactgcaac
     1081 aagcagccaa acaggcaacc aaaccgatcg tcaaagtatc ttcgcgcaca cccactattc
     1141 cgattatggc agcgccgaag aaacaacaag accgccgtta caacattgcc ttgggcatgc
     1201 cagagttcca tctcaagaaa cccaagtttt cctggccgca gttttctgct ccgcgcatgc
     1261 cacgtatgcc gcagttctct gcaaattggc gcctcggtgc gttgattctt tcagtggtgt
     1321 tcgtggttgc tttggtgctc gcttttcaac tcccatattt ctatgtccct caggcgtttg
     1381 tctctggaaa tgctcgcatt ccaagcgaag agattaatgg cattatgggc gtttcgaatc
     1441 aaaacatttt cacggttcag cccgatgaac tcgaacgtcg cttgcgctta aattaccccg
     1501 agatcacttc tgcacaagtg gatgtttacc ttcccaacta tgtctatgtc acagtaacag
     1561 aacgtcagcc tgtggtcatg tggcgtcaga atggcggctt tacctggatc gatgcaacag
     1621 gcgtcgcctt tagaccacga ggcgaagcgg cggctctggc agttgtggac gctgttgatg
     1681 caccgcctgc tggcgttcaa acatcggcgg atccctatag cccgccgcct ttcatccaaa
     1741 aagaatatgt ggatgccgct ctcgctcttg ctccgctcgt gcctgctggc tccaccctaa
     1801 cctattccgc cgccgatggc tttagctgga aagacccacg tggctggacc gttgcctttg
     1861 gcaccacggc gaatgatgtg ccgttgaaga ttcgtgttta tcaatctctg gtcgactcgt
     1921 tgactcaaaa aggcaaaatc cctgaattta ttagtgttgt gtatcccgat ggtccttact
     1981 atcgtatgaa gaaagccgta actgttgaag aagaccagac ggctcaaact gaccaatcca
     2041 ctgaagaaac cgtagtagag aacccgtaac atggaagaga tcgtagtagg cattgatgta
     2101 ggcacgacca aaatctgcac cctcgttgga cgagtggagg acgataagtc cattcgtatc
     2161 ttgggggttg gaattgagcc gtctgaagga atccgcaaag ggattgttgt agaccttgcg
     2221 gcggcttcac gcgccatcaa gcgttcggtt gaaaaagcgg agagcacatc gagccttgag
     2281 attaccactg cctttgtcag ccttgccggg gcgcatgtct cttcgatcaa tagccgtggc
     2341 tcggcgggca tcccaagcgg tgtgatcgat attcac
//
LOCUS       DAOREF010000499         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33446_length_2370_cov_2.406048, whole genome shotgun sequence.
ACCESSION   DAOREF010000499 DAOREF010000000
VERSION     DAOREF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33446_length_2370_cov_2.406048"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..876)
                     /locus_tag="PKI33_10510"
     CDS             complement(31..876)
                     /locus_tag="PKI33_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNM37490.1"
                     /translation="MNKTAPLLALFTFLSLVASCSTSQPVSNENESQSASIENSNAST
                     IPTVDINEPVINIPSTNETAPAGILEQINWAPSGGGDTTVTPTPNPGCVQECSVNIKD
                     SHIFLTNFNPNQKVRIVFYTRSDQTMTCMMFLIEYVTNSIVQVDESGNFSAKLSDTNK
                     PLSVVVLDYNTNEKIWGFEYQYTPCDISYYNACSGAPKQRLKIDEKAYVCTQKDSVKL
                     RSDAGKEYSEIKALIPGAEVTVIDGPLCADNWSWWQVRTESGFIGWMSEGGDEVDEYF
                     LCPLP"
     gene            complement(873..>2370)
                     /locus_tag="PKI33_10515"
     CDS             complement(873..>2370)
                     /locus_tag="PKI33_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37491.1"
                     /translation="NRVRNITTTIIKNARPRDYILYTSVFLLLLSAIGYFFWYNNLRY
                     DRAIASGKLNVLVVPFVEQHAWGYTESDLGWSLAQIFAEGLKTSYTTSGLETNVKILG
                     PADKVPTIFGFNEDHLVHSAETVSEKINGQVVIYGVLSQDEYGDTVAQVKVYISPTNF
                     GEAQELISDSMMGDLSLGSFVLTGETAKGADLEAQNEDLRNRLAIFASLINFLGAYLG
                     DDFSNAQKYIEAAGDSILWNDPNGLEVIQLLQGNLELRRARVSIVQKDLEGTQASIDL
                     ARTYFGNARETALKNNKGQYARAYLGLAGAENLAAVAEANINGDPSAINTDALKETLA
                     LLEQAEQANYAPDSADVSVKANYGRAQVNLAYFAKTGNSDYLTEAKKYYQFVVEEYKR
                     TDNKRIVDYTALSHSGLGLIASQENQVTVAIENFTSAYQLTGNPSLKIQCLVNMGDIY
                     FANKMYAEALKVYQYALSRRNDLENAISAERIDLIEERINFIKNGGTL"
BASE COUNT          595 a          522 c          540 g          713 t
ORIGIN      
        1 tagtggtgat ttcatgcagt ttcgcgattc ctaaggaagc ggacacaaga aatactcgtc
       61 tacttcgtct ccaccctctg acatccagcc aataaagcct gactcagtcc tgacttgcca
      121 ccacgaccaa ttgtctgcac ataaaggacc atcgatcaca gtcacttcgg ctccggggat
      181 cagggctttt atttcagaat attccttacc ggcatcggat ctgagcttta cagaatcttt
      241 ttgagtgcat acataagcct tttcgtcgat tttcaaacgc tgtttcggcg cgcctgaaca
      301 agcattgtaa taagagatgt cacatggtgt gtattggtat tcaaaacccc atattttttc
      361 gttcgtattg taatcaagca ctacaacaga taaaggttta ttggtgtcac taagtttggc
      421 agaaaaattg ccactctcat ctacctgaac aatcgagttt gttacgtatt caatgagaaa
      481 catcatgcat gtcatagtct gatctgagcg agtatagaaa acaattctca ctttttggtt
      541 cgggttgaaa ttggtgagaa atatatgtga gtcttttata ttgacgctac attcttgaac
      601 acatcccgga ttaggagttg gcgtcactgt tgtatctccg ccgccgcttg gcgcccaatt
      661 gatttgttcg agaataccag caggggcggt ctcgtttgtt gatgggatat ttatgacagg
      721 ttcattgatg tcaacagtgg gaatggtgga agcattgcta ttttcaattg aagcactttg
      781 gctttcattt tcattactta ccggctgtga tgttgaacag gatgctacaa gagacaagaa
      841 cgtaaataga gcgagaagtg gagctgtttt attcatagcg ttcctccatt cttgataaag
      901 ttaattctct cttcaatgag atcgattctc tccgccgaga tcgcattttc aagatcgttc
      961 cttcgactta gcgcgtattg atataccttt aatgcttctg catacatttt gttagcaaaa
     1021 taaatatcac ccatgttgac aagacattgg attttgaggc tgggattgcc ggtcagttga
     1081 taggcagatg tgaaattctc gatcgcaaca gtcacttggt tttcttgaga ggcgataaga
     1141 cccaaccctg aatgggacag ggcggtgtag tctacgatcc gtttattgtc tgtcctttta
     1201 tactcttcca ctacgaattg ataatatttt tttgcttctg tcaggtaatc actgttgcct
     1261 gtttttgcaa aatatgccag attgacctgt gccctgccat agttggcttt gacacttaca
     1321 tctgcagagt cgggggcata atttgcctgt tctgcctgtt cgaggagagc gagtgtttct
     1381 ttgagcgcat cggtattgat cgcggaagga tccccattga tatttgcctc tgctactgca
     1441 gctaggtttt ccgccccagc cagcccaaga tatgcccgtg cgtattgccc tttgttgttt
     1501 ttcaaggcag tctcgcgtgc gtttccaaag taagtacgcg caagatctat ggatgcctgc
     1561 gtgccttcca ggtctttttg aacgatcgac actctggcgc ggcgaagttc caggttgcct
     1621 tgcagcagtt ggatgacttc caagccattg gggtcattcc aaagaattga atcacctgcg
     1681 gcttcaatgt acttctgggc attggagaaa tcatccccaa ggtaggctcc cagaaaatta
     1741 atgagggatg cgaaaatagc aaggcggtta cgcaaatctt cgttttgcgc ctctagatcg
     1801 gctcctttcg ccgtttctcc tgtcagcaca aaagaaccga gcgatagatc tcccatcatg
     1861 gagtcggaga tcagttcctg cgcttcgcca aaattcgttg gggagatgta caccttgacc
     1921 tgtgcaacgg tatccccata ttcatcttgt gaaaggactc catagatcac gacttgacca
     1981 ttgatctttt cggagaccgt ttcagcggaa tgaaccaaat gatcttcatt gaacccaaaa
     2041 atggttggaa ccttatcggc aggaccaagg attttgacat tggtttcgag tcctgatgtt
     2101 gtataggatg ttttcaaacc ttctgcaaag atttgagcaa gactccagcc aaggtcactt
     2161 tcggtatacc cccaggcatg ttgttcgaca aatggtacga ccagtacatt taacttgccg
     2221 ctggcaattg cccggtcgta acgcaggttg ttgtaccaga agaaatagcc gatcgcagat
     2281 aataataaaa gaaaaacaga tgtgtatagg atgtaatcgc gcggacgggc gttcttgata
     2341 atggtggtcg ttatatttct tacacgattg
//
LOCUS       DAOREF010000500         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33516_length_2366_cov_3.858503, whole genome shotgun sequence.
ACCESSION   DAOREF010000500 DAOREF010000000
VERSION     DAOREF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33516_length_2366_cov_3.858503"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PKI33_10520"
     CDS             <1..568
                     /locus_tag="PKI33_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNM37492.1"
                     /translation="YQTVFSREPGSAAAPTAGLHFTPELLAKIQEMGVKTAYVTLHVG
                     LDTFAPVNEDDPEEHKIHSEWCHLSQETANAINETKRDGGRVIAVGTTSVRTLESAGK
                     DLILDVRSPNTEYRISPFTGPTTLYILPGYRFKVVDAMITNFHLPKSTLLMLVSAFAG
                     REKILETYNLAIREGYRFYSFGDAMFIE"
     gene            592..1257
                     /locus_tag="PKI33_10525"
     CDS             592..1257
                     /locus_tag="PKI33_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNM37493.1"
                     /translation="MNLEQHNKQIIACRKCPRLVEWREEVAQVKRKAYINEEYWGKPV
                     PGFGDPKARVLVVGLAPGAHGSNRTGRQFTGDASGNFLYPALHKAGFANQPTSESRSD
                     GLALKDMYITASGRCAPPDNKPSPEELNNCQPFLERELEIINPKVIVCLGRIAFERIL
                     KIYSEKSSVYKFAHGASYQLANGKWLLCSYHPSQQNTSTKKLTPTMFDAIWVKAKQLA
                     DRN"
     gene            1263..2009
                     /locus_tag="PKI33_10530"
     CDS             1263..2009
                     /locus_tag="PKI33_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM37494.1"
                     /translation="MKKINWKIVAAVITAVLLVLVAGFVFWASDASAATDVALQALNS
                     DSKVNVSAENGWVIFTPAENSRPETGFIFYPGGKVDYRAYAPILKMISAKGYFVAVVP
                     VPLNLAFFDINAASRVEAAYPEVKNWFVGGHSLGGVAASSYAASHEEIRGVVFWASYP
                     ADDSLKTKSIPAVSIYGTNDGLATDDKLDASKALLLEGTKFVPIDGGNHAQFGSYGPQ
                     AGDNEATISPEDQWLQATDATVAFFAEVLK"
     gene            2028..>2366
                     /locus_tag="PKI33_10535"
     CDS             2028..>2366
                     /locus_tag="PKI33_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM37495.1"
                     /translation="MKRWKKILLFAFAFACFTITLGPFAVPVPKLDGLVSEQEFVEPD
                     SKFIEINGVKIHYKEKGSGERTFILLHPFGGSTYSWRNVMDEFAKYGKVIAYDRPAFG
                     LTERPMPGDWE"
BASE COUNT          616 a          521 c          589 g          640 t
ORIGIN      
        1 ctatcaaact gtcttttcga gagagccggg gtctgctgcc gctcccacag cagggctaca
       61 ctttacgcct gaactgctcg caaaaattca agagatgggc gtaaagaccg cctacgttac
      121 tttgcacgtt ggcttagata cctttgcgcc agtcaatgaa gatgaccccg aagaacacaa
      181 aattcattct gaatggtgtc acctttcaca agagacggca aacgccatca acgaaaccaa
      241 acgcgatgga ggaagagtca tcgcggttgg tacaacatcg gtgcggacgt tggaaagcgc
      301 cggaaaagac ttaatacttg atgttcgctc accaaatacc gaatatcgaa tatcgccatt
      361 tacaggacct actactttgt acatcctccc tggctatcga tttaaagtgg ttgatgccat
      421 gatcacgaat tttcacctgc ctaaatccac gctcttaatg ctggtcagtg cctttgctgg
      481 gcgcgagaaa attcttgaaa cgtataatct cgcgattcga gaaggctatc gcttctattc
      541 tttcggtgat gcaatgttca tcgaataacc ttatattttt tacttattcc tatgaaccta
      601 gaacaacata acaaacaaat cattgcttgt agaaaatgtc cgcgtttggt ggagtggcgc
      661 gaggaagtgg cgcaagtcaa acgcaaggca tatatcaacg aggagtattg gggaaaacca
      721 gtgccaggtt ttggcgaccc gaaggcacgt gtgctcgtgg ttgggcttgc tcctggggcg
      781 catggttcga accgcacagg tcgtcagttc acgggtgatg cttctgggaa ttttttgtat
      841 ccggcgttgc acaaggctgg gtttgcgaac cagcccacgt ctgagtctcg aagcgatgga
      901 cttgctctaa aggatatgta tatcaccgcg tcgggtcgtt gcgctccgcc agataataag
      961 ccgagtccag aagagctaaa taattgtcag ccttttcttg aacgtgaatt agaaattatt
     1021 aatccgaaag tcattgtttg cctgggtcgg atagcttttg agaggattct aaaaatatat
     1081 tccgaaaaaa gttcggtata taaattcgct cacggcgcgt cttatcaact ggcaaatggg
     1141 aagtggctgc tttgttcata ccaccccagc cagcaaaata cctctacaaa aaaactgacc
     1201 ccaacgatgt tcgatgcgat ttgggtgaag gcaaaacaac tcgcagatag gaattgatgt
     1261 gcatgaaaaa aataaattgg aagatcgtag cggcggtgat caccgccgtt ttgttggtac
     1321 ttgttgccgg ttttgtgttt tgggcaagtg atgcttcagc cgccactgat gtggctttgc
     1381 aagcattgaa ctcagattcg aaagtgaatg tttcggctga gaatggctgg gtgattttta
     1441 ccccggcaga aaattcacga cctgaaacgg gctttatttt ttaccctggt ggtaaagtgg
     1501 attatcgcgc atatgcgccg atcttgaaaa tgatttctgc caaggggtat ttcgtagcgg
     1561 tagttccggt tccattgaat ttggctttct ttgatatcaa tgcagcttct cgggtagaag
     1621 cggcgtatcc tgaagtcaag aattggtttg taggcgggca ctctctgggc ggcgtggcgg
     1681 catcttctta tgcggcaagt cacgaagaaa ttcgaggcgt ggtgttttgg gcttcgtatc
     1741 cggctgatga ttctttgaag accaagagca ttcctgcggt ttcgatctat ggcacaaatg
     1801 acggacttgc aacagacgat aagttagatg catccaaagc tttgctgttg gaaggtacaa
     1861 aatttgttcc gattgatggt ggaaaccacg ctcaattcgg ctcttacgga cctcaagccg
     1921 gtgataacga agcgactatt tctcccgaag accagtggct tcaagccacc gatgcaaccg
     1981 ttgctttctt tgctgaggtt ctgaaataaa tcgtctatcg tctaaccatg aaacgctgga
     2041 aaaaaattct gttattcgct tttgcttttg cctgctttac catcacgctg ggaccttttg
     2101 ccgtccctgt tccaaaattg gatggtttgg taagcgaaca agaatttgtt gagcctgata
     2161 gtaaattcat cgagatcaac ggcgtgaaaa ttcattacaa ggaaaaaggt tctggtgaaa
     2221 ggaccttcat cctgttgcat ccttttgggg gcagcacgta ttcctggcga aacgtaatgg
     2281 atgagttcgc aaaatatggc aaagtcatcg catacgaccg cccagccttt gggctaacag
     2341 aacgtcccat gcccggtgac tgggaa
//
LOCUS       DAOREF010000501         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33517_length_2366_cov_3.847685, whole genome shotgun sequence.
ACCESSION   DAOREF010000501 DAOREF010000000
VERSION     DAOREF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33517_length_2366_cov_3.847685"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PKI33_10540"
     CDS             <1..774
                     /locus_tag="PKI33_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM37496.1"
                     /translation="TFILLHPFGGSTYSWRNVMDEFAKYGKVIAYDRPAFGLTERPMP
                     GDWETNPYGMKANVEILREMLDAFGIEKAVLMGNSAGGGVAVAFALEYPERIDELILV
                     APGVGGGYGPQFPAWALPIMWTPQMRHLGPLMMRDYQESLPRTVEREWFDQSKLTDEI
                     RAKHLQILTIKNWDKAFYELTFAPAYPELRPLLPNLKVPALIVAGQEDRLIRSWYFEA
                     ISTEIPEAQLKTIPQCGHVPQEECPTEFMEVVLKYLHYQ"
     gene            795..1259
                     /locus_tag="PKI33_10545"
                     /pseudo
     CDS             795..1259
                     /locus_tag="PKI33_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
     gene            1256..2290
                     /locus_tag="PKI33_10550"
     CDS             1256..2290
                     /locus_tag="PKI33_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37497.1"
                     /translation="MTLGRIAFLGSGETSLAGGRIFETLARLIPDPLRIAILETPAGF
                     ELNASLVAGRVADFLKTRLGNYKPVIDLIPARKKGTEFSPDNPEILKPLLQANMIFMG
                     PGSPSYVARQLKDTLAWDIIRARHRQGATLIFASAATISVGRWVLPVYEIYKVGEDVH
                     VKDGLDFFSDFGMQVSFVPHWNNAEGGVDLDTSRCFIGMERFNQWRNLTPSDNIIVGL
                     DEHSGIIMDCEKGVCDVHGVSSVSVLKSDTSEMYASGSSFSLMELGNCTPPNPIEKGI
                     RPEVWEMIASAQSAETDGPSEEVLALLEQRQQARANKDFAASDRLRDQIAALGWTVQD
                     SKAGQTLVKK"
BASE COUNT          590 a          591 c          571 g          614 t
ORIGIN      
        1 accttcatcc tgttgcatcc ttttgggggc agcacgtatt cttggcgaaa tgtaatggat
       61 gagttcgcaa aatatggcaa agtcatcgcg tacgaccgcc cagcctttgg gctaacagaa
      121 cgtcccatgc ccggtgactg ggaaacaaac ccatatggca tgaaagccaa tgtcgaaatt
      181 ttgcgtgaaa tgctcgatgc ctttggcatt gaaaaggctg tccttatggg caactcggcg
      241 ggcggtggcg tggcagtagc ctttgcgttg gaatacccag aacgcattga tgagcttatc
      301 cttgtcgcgc ccggtgtggg tggtgggtat ggtccgcagt tcccggcgtg ggcattgccg
      361 attatgtgga ctccgcaaat gcggcatctc ggtccgctca tgatgcgcga ttatcaagaa
      421 agcctgccgc gcacagtgga acgtgagtgg ttcgaccaat ccaaactcac cgatgagatt
      481 cgtgcgaaac atttgcaaat tctcacgatc aaaaattggg acaaagcctt ttacgaactc
      541 acctttgcgc cagcgtaccc tgaattacgc ccattgctgc cgaacttaaa agtccctgcc
      601 ctcattgtag ctgggcagga agatcgtctc attcgctcgt ggtattttga agccatctcc
      661 actgaaattc ccgaagcgca actcaagacc attcctcaat gcgggcatgt gccacaagag
      721 gaatgtccaa ccgaatttat ggaagttgta ctaaaatatt tacactacca atgagtactc
      781 tattgctgca aaacggtcaa cttgcaccag atttttcact agaagatata aacggaaacc
      841 tcatccgcct ttctgattat cgaggaaaga aaccggtcat cctttccttt ttgcgtggtt
      901 ttatctgacc acattgccgc gcgcagttgg cgcgcttccg cgattcgtat gggggttttt
      961 ccgaacgaaa tgccgaattg attgccgtag gtcctgatgg gcttgaacaa ttccagcgtt
     1021 attggcagcg tgaagacctt accttcattg gcgtgccaga cccagaaaat agtgttgcca
     1081 aattgtacaa gcaagaagta aacctattta aattcggtcg tatgcccatg aattgtattg
     1141 tagacctcaa tggctatatc cgttatattc attacagcgc cgatcgcaaa gattacccag
     1201 atattaaaat tttctttgat gtaatagatg agttgaataa agcatccagt aaagtatgac
     1261 acttggacgc attgcctttc tcggttccgg cgagacttct cttgccggag gacgcatctt
     1321 cgaaaccctc gcccgcctca tcccggaccc gttgcggatc gccattctcg aaacgccagc
     1381 tggctttgag cttaacgcct ccctcgtcgc gggtcgcgtc gcagactttc tcaagacacg
     1441 cctaggtaac tacaaaccgg ttatcgacct gatccccgcc cgcaaaaaag ggacagagtt
     1501 cagccctgat aaccccgaaa tcctcaagcc tttattgcaa gccaacatga tcttcatggg
     1561 acccggcagc cccagctatg tggcacgtca actgaaagat acgctggcat gggatattat
     1621 ccgtgccaga catcgtcaag gcgcaacctt gatttttgcc tccgctgcta cgatttctgt
     1681 gggacgttgg gttttgcccg tctatgaaat ttataaagta ggggaagatg ttcatgtgaa
     1741 agatgggctt gatttcttct ccgattttgg catgcaagtc tccttcgtgc cgcactggaa
     1801 caatgccgaa ggcggcgtgg atcttgatac tagccgttgc tttatcggca tggagcgctt
     1861 caaccaatgg cgcaatttga cgccatccga taacatcatc gttggtttag acgaacattc
     1921 aggcatcatc atggactgtg aaaaaggcgt ttgtgatgtg catggcgtca gctcggtttc
     1981 tgttttgaaa agtgacacct ctgaaatgta cgcctctggc tccagttttt ctttgatgga
     2041 acttggcaat tgcacaccgc cgaatcctat tgagaaaggc attcgcccag aagtatggga
     2101 gatgatcgct tctgctcagt ctgctgaaac agacggtccc tctgaagaag tgctggcatt
     2161 gctggaacaa cgtcaacaag cccgtgcaaa taaagatttt gccgcgtcag accgcttgcg
     2221 tgatcaaatc gctgctcttg ggtggaccgt acaagactca aaagcagggc aaacattggt
     2281 caagaaataa aaaagaaccg cctgcagagg cggttctttt tatgaaaaat actaatgctt
     2341 ggggctattt cctctgcctt tgccat
//
LOCUS       DAOREF010000502         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33570_length_2364_cov_7.194456, whole genome shotgun sequence.
ACCESSION   DAOREF010000502 DAOREF010000000
VERSION     DAOREF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33570_length_2364_cov_7.194456"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..759)
                     /locus_tag="PKI33_10555"
     CDS             complement(349..759)
                     /locus_tag="PKI33_10555"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HNM37498.1"
                     /translation="MKAALSGFQDFLVLPTSSLLTSGKSDDIPRENAHRLFEYVDENE
                     ELFRVLFQGRPIIYQSMLDQARQSAINLLHEVMDEHDSPIPFRIMANYLTTSTIELMK
                     LWLEDGKPYSVDIMGRYLVQMVFKPMRNILRENL"
     gene            1061..2266
                     /locus_tag="PKI33_10560"
     CDS             1061..2266
                     /locus_tag="PKI33_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNM37499.1"
                     /translation="MEDFNYQKTNQLSIINYILWNLVGIGLGSLIGAIAGSFMYAQHY
                     LIKFLWETVPDHLGGLNPLHTFITCVVGGLIVGVVRKHLGDEPQSIHAVLRSIKTGEN
                     VPGIKTVPAAFLLSLISLGFGAALGPEAALIGIAVGFSSWTGETIARYAKKLNLADLQ
                     KPWSKIPGYLAMVSGFTMFALVAMPMFSLTYSYFPYQYSLAGDEIFISIVLGFMGLLV
                     GKTFSLISNHLDKLLIPIKNKPILTSLLGGVLLGGLGMLSPLILFSGQIGFSFLFENG
                     LEMSGLFLIMIGVLKMISTKTNSATGWKGGEFFPVMFASAVIGLGISNLIPGINPMVA
                     IAALMAATVTIVMDNIYIALAVVLLFLPVNLIIPMLVASLVAMVTTKNFIFSPKFAIQ
                     KVSVKPEKE"
BASE COUNT          671 a          477 c          475 g          741 t
ORIGIN      
        1 ttaaattctt ttagaaggtt ttccacccaa tcagcagaaa atcccgccga atatcgaaac
       61 cacctatgaa ttggcagttt catattatca ataaaagtgc ctgaacgagt cgctttttga
      121 gtgtcagtag gtaagtcgaa taaggttttt tgcataaagc aattataagc gttgtgcggg
      181 gatatttaga aataccacaa ttttgcaaga agcaacccaa cggcttgcgt tacccgcaag
      241 tgggcgggac tggattctgt ttgaaaacag tataaaccca aagcgagaaa aaagcctgta
      301 aaacgcgcag actcccactt gtcgggtgca cgctttgtta gattgccatc aaagattctc
      361 tctcaatata tttcgcatcg gcttgaaaac catttgaact aaatatctcc ccatgatatc
      421 aactgaataa ggtttcccat cctccaacca taatttcatc aattcaatgg ttgaagttgt
      481 caaatagtta gccatgatac gaaaggggat tggagagtca tgttcatcca ttacctcgtg
      541 aagtaaattg attgcacttt ggcgcgcttg atccagcatt gactggtaaa tgatcggtcg
      601 tccttgaaat aaaactctga ataattcttc attttcatca acgtactcaa acagcctgtg
      661 agcattttcc cgtggtatgt catccgattt gcctgatgta agaagtgaag aagtgggcaa
      721 taccaaaaaa tcttgaaatc cagagagagc cgccttcatt acatcaatga gcaattcatc
      781 tttgccagca tagtgtcgat aaaacgtgcg gtgcccaata tctgcccttt cagtgatttc
      841 ctgaatcgtg atttcgtcat actctttttt gaaattaact caaccaaggc ttcatataac
      901 agcttacggg ttcgcctgat tcgtcgatct tcttgcgaca tagcctgttc cttctaatat
      961 ttttctaaca gatttgatat tgacgtattt atacacttat ggcatattta cgccataagt
     1021 catgcgtatg tattatcatt ataaaggcat atctaacaat atggaagact tcaattatca
     1081 aaaaacgaat caactatcaa taataaacta cattctttgg aatttagttg gtattggatt
     1141 gggcagttta attggggcaa ttgcgggatc attcatgtat gcccaacatt atctgattaa
     1201 attcctatgg gaaactgtcc ccgatcactt gggcggtctg aatcccctac atacttttat
     1261 cacctgtgtt gttggcgggc tgatagttgg ggttgtgcga aaacatctgg gagatgaacc
     1321 ccagtccatt catgctgttc ttcggagcat aaaaacaggc gaaaatgtgc caggtattaa
     1381 aactgttcct gctgcatttt tactttccct aatctccttg ggatttggag ccgcattagg
     1441 cccggaagca gctcttatcg gtatcgcggt gggttttagt tcctggacag gtgaaaccat
     1501 agcccgatat gcaaaaaagt taaacttggc tgacctgcag aaaccctggt caaaaatacc
     1561 cggatatctt gctatggtaa gcggttttac tatgtttgca ctggtcgcta tgccaatgtt
     1621 tagtctcaca tattcctact ttccatatca atattctcta gccggtgatg aaatctttat
     1681 ttccatcgtg ttaggtttta tggggctttt agtaggaaaa acgttctcac ttattagcaa
     1741 ccatctcgat aaactattga ttcccataaa aaacaaacct atcctaacca gtttactggg
     1801 tggagttttg ttaggtggcc tgggaatgct atcaccactg atccttttct ctggtcaaat
     1861 aggatttagt tttctttttg agaatggttt ggaaatgagc ggactatttc taatcatgat
     1921 tggtgtactc aaaatgatct ccaccaaaac aaactctgcc accggttgga aaggcgggga
     1981 gtttttcccg gtgatgtttg ctagtgcggt aattgggttg gggatttcta acttaattcc
     2041 cgggattaac ccgatggttg caatagcggc gcttatggca gccactgtta caattgtgat
     2101 ggacaatatc tatattgcat tggcagttgt acttctattt ctgccagtaa atttgattat
     2161 ccccatgttg gtggcttcac tcgttgctat ggttactaca aagaacttta tatttagtcc
     2221 gaaatttgca atccaaaaag tttcagtaaa acctgaaaaa gaatgaagca ataggtatta
     2281 ccagtagggg ttggtgattt cccctacgtc aaagatgtta tttatcatca ccaacttaca
     2341 tgaaattgaa attggcaatc taac
//
LOCUS       DAOREF010000503         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33575_length_2364_cov_3.496319, whole genome shotgun sequence.
ACCESSION   DAOREF010000503 DAOREF010000000
VERSION     DAOREF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33575_length_2364_cov_3.496319"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKI33_10565"
     CDS             <1..382
                     /locus_tag="PKI33_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM37500.1"
                     /translation="LSAVPRALREGAYAMGATKLETSLQVVLPAALSGIGAAFIIGIS
                     RAVGETMIVAIAAGSGPNFSFNPLEAAETMTGHIARISGGDLSYNSIDYNSLFSIALM
                     LFLVTLVLNLISQWVVNRFRERYE"
     gene            379..1614
                     /gene="pstA"
                     /locus_tag="PKI33_10570"
     CDS             379..1614
                     /gene="pstA"
                     /locus_tag="PKI33_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNM37501.1"
                     /translation="MSKQPSYPQGEAFYSVLRTRYQKAAVWQVIFLGSLFIAIVALTA
                     LLYNVVDGAFGYIAYEYKNDPSEFTSTPVAELDKEELLVILKENLSSGAYKKLDTEEK
                     MEERTQADLLSLFLERLVRIDTKQTWDLAESLTRGAEIRAEVADKYPDAKLEFRSWLT
                     PQFLVSPMSSRAEFAGVRTAILGSLWLVGIAIAFALPIGTGAAIYLEEYATKNWFTRI
                     IQTNINNLAGVPSIVYGMLGLALFVRVMEPFTSGSIFGVTDSNGRTILAAGLTMGVLV
                     LPVIIINAQEAIKAVPDSLRQAAYGVGATRWQTVWHHVLPNALPGILTGSILALSRAL
                     GETAPLIVVGASTFITLDPDSPFSKFTALPIQIYQWTTRAQSEFHSIAAAAIIVLLVL
                     LLTLNATAILLRNRFQKRW"
     gene            1627..>2364
                     /gene="pstB"
                     /locus_tag="PKI33_10575"
     CDS             1627..>2364
                     /gene="pstB"
                     /locus_tag="PKI33_10575"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNM37502.1"
                     /translation="MENNYALETNDLSIHYGTFTAVKGVNIKVERQKITAIIGPSGCG
                     KSTLLRSFNRMNDFVPGCNVTGELLLQGQNLYGKNIDPVEVRRKIGMVFQKPNPFPKS
                     IYENIAWGARINGFKGNMDELVESSLRSAALWDEVKDKLQQSAYALSGGQQQRLCIAR
                     TIAIQPEIILMDEPASALDPISTLRIEELMQELKKNYTILIVTHNMQQAARASDYTAM
                     MMIDDQRAGGVIEFDDTKNIFTRPKDKR"
BASE COUNT          555 a          690 c          558 g          561 t
ORIGIN      
        1 cttgagcgct gttccgcgtg ccttgcgtga aggtgcctac gccatgggcg cgaccaaact
       61 tgaaaccagt ttacaggtgg tacttcctgc ggctctctct gggattggag cagccttcat
      121 catcggcatc tcccgcgctg tcggtgagac aatgatcgtc gccatcgccg ccgggtctgg
      181 accgaacttc tcgttcaatc cgctcgaagc cgccgaaacc atgaccggtc acattgcgcg
      241 catcagcggc ggtgacttgt cctataactc gattgattac aacagcttgt tctccattgc
      301 attgatgtta ttcctcgtca cattagtgtt gaatctcatc agccaatggg tggtcaatcg
      361 cttcagggag cgttacgaat gagcaaacaa ccttcatatc cacaaggcga agcgttctat
      421 tctgtactgc gcactcgcta tcaaaaagcc gccgtatggc aggttatctt cctcggttcg
      481 ttattcatcg ccatcgttgc cctgaccgcc ctgctctaca atgtggttga cggtgcattc
      541 ggctatatcg cttacgaata taagaacgac cccagcgagt tcacgtccac gccggttgcc
      601 gagctggaca aagaagaatt gctcgtcatt ttgaaagaga acctctcctc aggggcttat
      661 aaaaaactgg ataccgagga aaaaatggaa gaacgcactc aagcagattt gcttagcctc
      721 ttccttgaac gcctcgtccg tatcgacacc aaacagactt gggacctcgc cgaatccctc
      781 acccgtggag cagagatccg cgctgaagtt gccgataaat atcccgacgc caaactggaa
      841 ttccgctcgt ggctcacacc acaattcctc gtctcgccca tgtcttcgcg ggcagagttt
      901 gccggggtgc gtactgccat tcttggttcg ctttggttgg tcggtattgc catcgccttc
      961 gccttaccca tcggcacggg tgccgctatt taccttgaag aatacgccac gaagaattgg
     1021 ttcactcgca ttattcaaac caatattaat aaccttgccg gggttcccag cattgtatat
     1081 ggaatgctgg ggctcgcctt attcgtgcgt gtaatggagc ctttcaccag cggctcgatc
     1141 ttcggcgtaa ccgactcgaa cgggcgcacc atcctcgccg ccgggttgac gatgggcgtg
     1201 ctggtgttgc cggtcatcat catcaacgca caagaagcta tcaaggccgt cccagattca
     1261 ctgcggcaag ccgcctacgg tgtcggtgcg acgcgctggc aaaccgtttg gcatcatgtt
     1321 ttgccgaatg ccttgccggg catcctcacg ggcagcatcc tggctttgtc tcgtgcgttg
     1381 ggtgaaactg ccccgttgat cgtggtgggc gcctccacct tcatcacgct cgacccggat
     1441 agtcccttct caaaatttac ggcgctgccg atccaaatct atcaatggac aactcgcgcg
     1501 caatctgaat ttcattcgat tgccgcggct gccatcatcg tcctgctggt gttgctcttg
     1561 accctcaacg caaccgccat cctgcttcgc aatcgcttcc agaaacgctg gtaacggaac
     1621 tcaatcatgg aaaataatta cgcactcgaa actaacgatc tatccattca ttatggaaca
     1681 ttcactgccg tcaaaggcgt gaacatcaaa gtggaacgtc aaaagatcac tgccatcatc
     1741 ggtccgtctg gctgtggcaa atccacgcta ttacgttctt tcaaccgcat gaatgacttc
     1801 gtgccgggtt gcaacgtcac aggtgaattg cttttacaag gacaaaatct ctacggcaaa
     1861 aatatagacc ccgtggaagt gcgccgcaag atcggcatgg tctttcaaaa accgaacccc
     1921 tttcccaaat ccatttacga gaacatcgct tggggcgctc gcatcaatgg attcaagggc
     1981 aatatggacg aactcgttga aagctctcta cgttccgccg ccctttggga tgaagtaaaa
     2041 gacaaacttc aacaaagcgc ctatgccctc tcgggtggtc agcaacaacg tctctgcatt
     2101 gcacgcacca tcgccattca acccgaaatc atcttgatgg acgaacctgc ctctgcgctg
     2161 gacccgatct ccacgctgcg catcgaagaa ttgatgcaag agttgaaaaa gaactacacc
     2221 attctcatcg tcactcacaa catgcaacaa gccgcgcgcg ccagtgacta tactgccatg
     2281 atgatgatcg acgaccaacg tgccggcggt gtgatcgaat ttgacgatac caagaacatc
     2341 ttcacccgcc ccaaagacaa gcgg
//
LOCUS       DAOREF010000504         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33795_length_2355_cov_4.564348, whole genome shotgun sequence.
ACCESSION   DAOREF010000504 DAOREF010000000
VERSION     DAOREF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33795_length_2355_cov_4.564348"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKI33_10580"
     CDS             <1..286
                     /locus_tag="PKI33_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37503.1"
                     /translation="LENLRTQTQRRHELLVKAVDAVGQAVEHSSYSRNPSEIKTAINN
                     LLDAIKENSPAVEQPEAPKVGGSAPRTPPASNQKIEDLTNTLLDLLKPRK"
     gene            283..1338
                     /locus_tag="PKI33_10585"
     CDS             283..1338
                     /locus_tag="PKI33_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37504.1"
                     /translation="MTKSFIETTETHEGWQSVFSFQAFHYPAEEWLKMKWGFNAGALL
                     DEAMDRNRLFLESQGINETLYFDENLPTRTLVIRGIKRPGNGLQMSVLGKVNAASQEQ
                     AKQSAEKYAREIRSTFPHDFHLLPTETKLAHDKMAGNDLLVKRPSIVSIQRENTFIPP
                     MRGFHYLNGFWQTSIRSNEQIWRALSNMDQATMFNINLQPTVLLEDEKELLLEIKKKV
                     LDVEEKPAIFLPYYPWVENCIKRRLAPWKKFFLLQVHIVTEQEVDENLSRSIGSALTR
                     DTDTSPLPGFHAMRPETESEAEEWIEDLRLLSLTPPQRRMDDLVDLDEAFSVFRLPLR
                     PEAGLPGANFIEPSSIN"
     gene            1343..>2355
                     /locus_tag="PKI33_10590"
     CDS             1343..>2355
                     /locus_tag="PKI33_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNM37505.1"
                     /translation="MNNQTPREIVHVRVTRHMPQGLSILLDNGEQGIIRAREISWKEK
                     EDSNWKDNYPIGWDGYVVALPNKKGEVPEFSIRLLEYDPWEDFFEGLGKNRVLEGHVT
                     GVYEYGAFIEVDEGITGLLHKSRIPAKVQKSIHDLFWFGDKVRVVVHDVDHERRHIEL
                     SLAPVEDITGENPAGMQNQASAASEAASHHLGQKLPKKHILVVEDETPQSDAVCGWLQ
                     EMGQQVDCVQSAEDAIEFLTRVQPDFALVDVGLPGMSGTELAHHIFENYPQIQVANAT
                     DWARASEVRAELEAVMDRGGNLFYKPLVPEDLAKFLTGDVQRVEIASQEDVVPSRSAS
                     KWDA"
BASE COUNT          683 a          543 c          576 g          553 t
ORIGIN      
        1 gctcgaaaat ctgcgcacac aaacccaacg ccgccatgaa ttactggtca aagccgtaga
       61 tgcggtgggt caagccgtgg aacattcaag ttattctcgc aacccaagcg agatcaaaac
      121 tgccatcaat aatctattag atgccattaa agagaattct ccggcggtcg aacagccaga
      181 agcgcccaaa gttggcgggt ctgctccgcg cacaccacca gctagtaatc aaaagatcga
      241 ggatcttaca aacaccttat tagatcttct taaaccaaga aaatgaccaa gtctttcatt
      301 gaaacaaccg aaacgcacga gggttggcaa tctgtatttt cttttcaagc atttcattac
      361 ccagccgaag aatggctgaa aatgaaatgg ggcttcaatg cgggtgcatt acttgatgaa
      421 gccatggatc gaaaccgcct attccttgag tcacagggta tcaatgaaac gttgtacttt
      481 gatgaaaacc taccgacacg gacattggtc attcgcggga ttaagcgccc tggtaatgga
      541 ctacaaatgt ctgtgcttgg aaaggtcaat gctgcaagcc aggaacaggc aaagcaaagt
      601 gcagaaaaat atgcgcgcga aattcgctct acctttccgc atgattttca cctactgccc
      661 acagagacaa aattagctca tgacaaaatg gctggcaacg acctactagt gaaacgtcca
      721 agcatcgtat ccattcaaag agaaaatacg ttcatcccac ccatgcgcgg ttttcactat
      781 ctaaatggct tttggcaaac aagtattcgt tccaacgaac agatctggcg cgcgctctca
      841 aacatggacc aagcgaccat gtttaatatt aatcttcaac ccacagtact gttggaagat
      901 gaaaaagaac tattattaga aataaaaaag aaagtgcttg atgtagaaga aaaaccagcc
      961 atcttccttc cctattatcc atgggttgaa aactgcatca agcgtcgctt agcaccttgg
     1021 aaaaaattct ttctactaca ggttcacatt gttacagaac aagaagtaga tgaaaatctt
     1081 tcgcggtcca ttggctcggc gctcacacga gatacagata cctcaccgct gcccggcttt
     1141 cacgccatgc gcccagaaac agaaagcgaa gccgaagagt ggattgaaga ccttcgtctc
     1201 ttgagcctga cgccgcctca aagaagaatg gatgacctgg ttgaccttga tgaagccttt
     1261 tcagtcttcc gcctccccct ccgacctgaa gcgggtctgc cgggtgcaaa ctttatcgaa
     1321 ccttcgagca taaactaaga acatgaacaa tcagacacct agagagatcg tacacgtccg
     1381 cgtaacccgg catatgccac aggggctttc tattctgctg gataacggcg aacagggcat
     1441 catccgcgcc cgggagatct cttggaaaga aaaagaagac tcgaattgga aagacaacta
     1501 tcccatcggt tgggatggct atgtagtcgc attgcccaac aaaaaaggag aagttccaga
     1561 gttcagcatc cggttgttgg aatatgaccc ttgggaagat ttttttgaag ggcttggcaa
     1621 gaatcgagtt cttgaagggc atgttacagg ggtatatgaa tatggcgcct tcatcgaagt
     1681 ggatgaaggg attaccgggc tgcttcataa atcgcgtatt cccgccaaag tgcaaaagtc
     1741 tattcatgac cttttctggt ttggcgataa ggttcgggtt gtggtccatg atgtagacca
     1801 tgaacgcagg cacatcgagc tgagtctggc tccggttgaa gatattacag gagagaatcc
     1861 ggctgggatg cagaatcaag catcggctgc gtctgaggcg gctagtcatc atcttgggca
     1921 gaaactcccc aagaagcaca tccttgttgt tgaggatgaa acaccacagt cggatgcggt
     1981 ttgtggttgg ttgcaagaaa tgggtcagca ggttgattgt gttcaaagtg cggaagatgc
     2041 catcgagttc ttgacgcgcg tacagcctga ctttgcgttg gttgatgtgg ggttgcccgg
     2101 aatgagtgga acggaattgg cgcatcacat tttcgagaac tacccacaaa ttcaagtagc
     2161 caacgcaacg gactgggcgc gtgccagtga ggttcgtgca gagcttgaag cggtgatgga
     2221 tcgcggcggg aatttatttt acaagccact tgtacctgaa gacttggcaa aatttctaac
     2281 gggtgatgtt caacgagttg aaatagcaag ccaggaagat gttgtgccat cacgatctgc
     2341 atcaaaatgg gatgc
//
LOCUS       DAOREF010000505         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33836_length_2353_cov_4.511314, whole genome shotgun sequence.
ACCESSION   DAOREF010000505 DAOREF010000000
VERSION     DAOREF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33836_length_2353_cov_4.511314"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..958)
                     /gene="map"
                     /locus_tag="PKI33_10595"
     CDS             complement(212..958)
                     /gene="map"
                     /locus_tag="PKI33_10595"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNM37506.1"
                     /translation="MIATDEKDINGLRAAGKVCAQAMQLMMESAKPGMTTLELDQIGE
                     DFLKKNGAKSAPRVMYNFPGGTCISVAPVIAHGIPGEHVIQDGELINIDVSAELNGYF
                     GDTGASMVVGTPNPEYQKMLDATKATLDKVLTLVRAGKPLNIIGKTVQQEANKNGYNP
                     IFDLTGHGIGRQLHDKPSAVYNFHKPDDRRTLDNGLVLAIEPFFTTGQGHVVEKSDGW
                     SLKTVDNTIAAQFEHTVIITKGEPIILTLP"
     gene            complement(959..1456)
                     /locus_tag="PKI33_10600"
     CDS             complement(959..1456)
                     /locus_tag="PKI33_10600"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNM37507.1"
                     /translation="MTEQWMPENKEELISAIEREWKLLISLAETLTDEQMTTLDEGGW
                     SPKDNLAHLTEWMNILMGYHMDKRPYYDVVGVSQEELPQWSVEAINPILFKRNQLRPR
                     AEVMDHLKRVYGLLVKKLDETPFEELLAPRHPDRPDPLILWVLGDTTEHFQEHRETIE
                     RGIKT"
     gene            complement(1466..2152)
                     /locus_tag="PKI33_10605"
     CDS             complement(1466..2152)
                     /locus_tag="PKI33_10605"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNM37508.1"
                     /translation="MPSNLHLFSSPGEGDIDDIVEACRPYVEGKEEPTIAYLPLASLY
                     AERWLEMTEDAFKGLAELKTINTELMTQKAIEGMIRDADVVYVPGGNTFLLNHRLHAS
                     GVMPFLQKRIQSGLPYVGFSAGAILCGPNILTSKDLNAVSTSFFKGLNAFQFNVSPHY
                     PLDGYGQSVQDDWLADYHFFHDNPVIMLCDGSYIKSEKGKTSLVRGEAYILRKDSEKE
                     RLEEGQIITL"
BASE COUNT          575 a          579 c          552 g          647 t
ORIGIN      
        1 gaaggcttta ttttttcaga acatcggcag gcaatttcac tggagaccga ccccaccaaa
       61 ctgatagagt ccatgcgaaa ttacgaacac ccgcatgatg cagtgaaacg atggatgaag
      121 gctgaataag gatcttggat taaacaattt acgcatcagg taatacgaga tttgtattgc
      181 ctgatgcgta atgatttaac aagcgtgttt tctatggcaa cgtcaaaata atcggctcgc
      241 ctttggtgat gatgacggta tgctcaaatt gagccgcaat cgtattatcc acggttttca
      301 aagaccagcc atcagacttt tcgacgacgt gtccctgccc ggtggtgaag aaaggctcaa
      361 tggcgaggac caagccatta tccagtgtgc ggcggtcatc tggcttatga aagttataga
      421 ccgcggacgg cttatcgtgc agttgacgtc caatgccatg cccggtcaaa tcaaagatgg
      481 ggttatagcc gttcttgttg gcttcttgtt gaaccgtctt gccaatgatg ttgagcggtt
      541 tgccagcgcg gaccaaggta aggactttat ccaaggtggc tttggtggca tccaacattt
      601 tttgatattc agggttgggc gtgcccacga ccatagaagc accagtatct ccaaagtagc
      661 cattcagctc agcagagaca tcaatgttga tcagttcgcc atcttgaatc acatgctcac
      721 ccggaatacc atgtgcaatg accggcgcaa cgctgatgca agtgccgccg gggaagttgt
      781 acatcacacg cggcgcagac ttggcgccat ttttcttgag aaaatcctcg ccaatctggt
      841 ctagttcgag cgtggtcatg cccggtttgg cgctttccat catcaattgc atggcttggg
      901 cacagacttt gcctgcggct cttaagccgt tgatatcttt ttcgtcagtt gcgatcattt
      961 aggttttgat tcctctttca atagtctcac gatgttcttg aaaatgttcc gtcgtatcgc
     1021 ccaagaccca taagatcaaa gggtcgggtc ggtctgggtg gcgcggagca agcaattctt
     1081 caaagggagt ttcgtctaat tttttcacca gaagtccata gacgcgtttt aggtgatcca
     1141 tgacttctgc tcgcgggcgg agttgattgc gcttaaacaa gatgggatta atcgcttcta
     1201 cgctccactg aggcaactct tcttgcgaga ctccaacgac atcgtaatat gggcgtttgt
     1261 ccatatgata gcccattaag atattcatcc attcggtgag gtgagcaagg ttgtctttgg
     1321 gtgaccagcc gccttcgtcg agtgtggtca tttgttcatc ggttagcgtt tcggcaaggc
     1381 tgataagcaa cttccactca cgttcaatgg cagaaatcag ttcttcttta ttttcaggca
     1441 tccattgttc agtcatcgct tcctcctata acgtgattat ttgaccttct tcaagtcttt
     1501 ctttttctga gtcttttcgc aaaatgtacg cttcccctcg gacaaggctg gtcttgccct
     1561 tttcgctttt aatatatgat ccatcgcaca gcatgataac agggttgtcg tggaagaagt
     1621 gataatctgc cagccaatca tcctgcacgg actgaccata tccatccaac ggataatgcg
     1681 gcgagacatt gaactgaaat gcattcaagc ctttgaaaaa ggatgtgctg actgcgttca
     1741 aatctttcga ggttaggatg tttggtccac acaggatagc gcccgcgctg aacccaacat
     1801 aaggcaaacc agattgaatg cgcttttgca ggaaaggcat caccccacta gcatgtagac
     1861 gatggtttag caaaaaggta ttcccgccag gcacatacac aacgtctgca tcgcggatca
     1921 tgccttctat tgctttttgg gtcatcaact cggtgttgat cgttttcagt tctgccaacc
     1981 ccttgaaggc atcttcggtc atttccaacc aacgttcggc atataaagaa gccaacggca
     2041 aataggcaat ggtaggttct tcttttcctt ctacatatgg gcggcaagct tctacgatat
     2101 cgtcaatgtc cccttcaccg ggagacgaga agagatgtaa attcgagggc ataaggggat
     2161 tgtatcaaac aaaatcgtag gggcgactcg cgagtcgccc ctacgcgatc atgtacctaa
     2221 tttttccaaa atcacttgcc gcaactccgc ctgcacagat tcccatgcct gctcagagct
     2281 gaccaccacc cacctcttct gctcggcttt gaccatctcc aaatacccat cgcggactcg
     2341 tttgtggaaa tcc
//
LOCUS       DAOREF010000506         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33838_length_2353_cov_3.856832, whole genome shotgun sequence.
ACCESSION   DAOREF010000506 DAOREF010000000
VERSION     DAOREF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33838_length_2353_cov_3.856832"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKI33_10610"
     CDS             complement(<1..660)
                     /locus_tag="PKI33_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNM37509.1"
                     /translation="MSDLTPILVTGSHRSGTTWVGRMLAADASTAYISEPLNVHHRPG
                     VYRTSVKYWYTYITDENGADYLPAFNELLDFKYHTVRETLALRSFKDFLRMGRDFHTF
                     FNGSMQGRRILIKDPFAVFSTPWFEKQLHCKVVITVRHPGGFVSSLKRLGWNFDFNNF
                     LNQPLFMRDHLEGDRAAMEAMPGDDLVGQAALLWKIIYRFVHGTRSQFSQFHIVRHED
                     LS"
     gene            complement(653..2143)
                     /locus_tag="PKI33_10615"
     CDS             complement(653..2143)
                     /locus_tag="PKI33_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37510.1"
                     /translation="MLEKKQNYLNYALWVVNIALWMTVGLYAFVGIYSRYLADDYCEA
                     MRVSDASSPLEATIQRYMTPGWPRETMRYSNILFVGISELFGRYSMQVSIPLLILLWS
                     VGVTWLLHEIFLFLKLKISLKEELFLSSLFVFISFLMSSNLFQVIYWRSSAMTHFAPL
                     VFGSFFCAFLLNVFRQDEKKSISIWVYISSYFISFLLAGFSEPPVTVAITVCTFLMIY
                     FFFWDSSKNKNRRLILLTVTLAGVISGLAAMFLSPAVSDYQRGEPVDIVAVLLNSFLY
                     SFQFIVDLLKSFPLPLAVCLLLPALFFFVYSPTLQENTSPLNSRKLWSGILILFIVVW
                     ILIAASFSPSVYGQGYPAGRARFLGSSMFVAFIIFLGASLGMIFFNSLKLLSLPLQKI
                     AMFLFVLIGVVYPLRRDVGIYSSDLPESQKQAMYWDLRYAQIQRKISQGETDLQIPAF
                     SGIHGIKELDLRTNFVYNVCAAKLYGANSISTYSVKEKDLMEALSE"
     gene            complement(2162..>2353)
                     /locus_tag="PKI33_10620"
     CDS             complement(2162..>2353)
                     /locus_tag="PKI33_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37511.1"
                     /translation="YGKERGAAEKDFQDQWNELMPKKDLFLVTDFKDLELQPFLKAKL
                     STYPIFAEGNGYVIYQLKP"
BASE COUNT          780 a          513 c          489 g          571 t
ORIGIN      
        1 ggaaaggtct tcgtggcgca caatgtgaaa ttgggagaac tggctgcggg tcccatgtac
       61 aaagcggtaa atgatcttcc acagcaatgc agcttgccca acaaggtcat cgcctggcat
      121 ggcttccatg gcggcgcggt cgccttccaa atgatcccgc ataaatagtg gctggttgag
      181 aaaattgttg aaatcgaagt tccagccaag gcgtttgaga ctgctaacaa aaccgccagg
      241 atgccgaacc gtgatgacca ctttgcaatg taactgcttc tcaaaccacg gtgtagaaaa
      301 tacagcaaaa ggatctttga tcagaattcg ccgtccttgc atactgccat tgaagaatgt
      361 atggaagtca cgtcccatac gcagaaagtc tttgaatgaa cgcagtgcaa gggtttcacg
      421 cactgtatga tacttaaaat caagaagctc gttgaaagca ggtagatagt ctgcgccgtt
      481 ctcatcggtg atgtaggtat accaatactt gaccgacgtg cgatatacac cgggacggtg
      541 atgtacatta agcggttcgc tgatatatgc agtacttgca tcggcagcaa gcattctacc
      601 aacccaagtg gttccgctgc gatgcgagcc agtgacgaga ataggggtta ggtcactcac
      661 ttagtgcctc cataaggtct ttttctttca cgctatatgt actaatcgaa ttcgcgccat
      721 ataatttggc agcacaaaca ttgtaaacaa agtttgtgcg taaatctaat tcttttatgc
      781 catgtatacc tgaaaaagct gggatttgta aatctgtttc tccttgagag atcttacgct
      841 gaatttgagc gtatcgtaag tcccaataca tggcttgttt ttggctctct ggcaagtctg
      901 aagaatatat tccaacatct cttcgaagcg ggtaaaccac gccaatcaat acaaataaaa
      961 acatggctat tttctgtagg ggcaaagaaa gcaatttaag agaattgaaa aatatcatgc
     1021 ctaacgaggc gcccaaaaat ataataaatg ccacaaacat actgctgccc aaaaatcttg
     1081 cccgcccagc ggggtatccc tgcccataga cgctcggaga aaaacttgct gcgattaata
     1141 tccaaacgac aataaacaaa atcaatatgc ccgaccacaa cttacgggaa tttaatggag
     1201 acgtattttc ttggagggtt ggagaataaa cgaagaaaaa taatgcaggc aaaagtaaac
     1261 atacagctaa aggcaaagga aagctcttca acagatccac aataaactgg aagctgtata
     1321 aaaacgagtt aagtagaact gcaacaatat ccaccggttc gccacgctga taatcactca
     1381 cggcagggga taagaacatg gcagccaacc ctgaaatcac cccagcaagc gtgacagtta
     1441 acaagatcaa acgtctattt ttattttttg aagaatccca aaagaaaaag taaatcatca
     1501 aaaatgtgca tacggtaatg gcaaccgtta ccggcggctc tgaaaaccca gcaagaagaa
     1561 aagaaataaa atacgagcta atatataccc aaatagagat ggatttcttt tcatcctgcc
     1621 ggaaaacatt caacaaaaat gcacaaaaga aagagccaaa taccagaggc gcaaagtgtg
     1681 tcatggcaga tgaacgccaa taaataacct gaaacaggtt cgaagacatt agaaagctaa
     1741 taaatacaaa caacgagctt aagaataatt cttctttgag gctaatcttt agtttcaaaa
     1801 aaagaaaaat ttcatgaagt aaccaagtta ctcccacact ccacaacaaa atcagcaagg
     1861 ggatgctaac ctgcatactg tacctaccga acagttcact tatccccacg aataagatat
     1921 tggaatacct cattgtttcg cgcggccagc ccggggtcat ataacgttga atggtggctt
     1981 caagaggaga agaagcatct gaaacccgca ttgcctcgca atagtcatct gcaaggtagc
     2041 gggaataaat tccgacaaaa gcataaagcc caacggtcat ccataaagca atatttacca
     2101 cccaaagtgc gtaattcaag taattctgtt ttttttcaag catcatcaaa caatatcctg
     2161 tttagggctt caattgatag atcacatacc cattcccttc agcgaagatg ggataggtac
     2221 ttagcttcgc tttgaggaaa ggttgaagtt cgaggtcttt gaaatcggtc acaagaaata
     2281 aatctttctt gggcatcaat tcattccatt ggtcttggaa atctttttcg gcggcaccgc
     2341 gttctttgcc gta
//
LOCUS       DAOREF010000507         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33929_length_2350_cov_3.129847, whole genome shotgun sequence.
ACCESSION   DAOREF010000507 DAOREF010000000
VERSION     DAOREF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33929_length_2350_cov_3.129847"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKI33_10625"
     CDS             complement(<1..307)
                     /locus_tag="PKI33_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37512.1"
                     /translation="MKKYSLILIALLGVLACKALTPNPQEVETPVATDEPPSVIVQIT
                     DAPSEETAVTSSTRFTIVRLKPEDGDLAAMLASESKKAQALGQMPVVEFDATWCPPCQ
                     "
     gene            complement(330..848)
                     /locus_tag="PKI33_10630"
     CDS             complement(330..848)
                     /locus_tag="PKI33_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37513.1"
                     /translation="MKKIFAFAILSVLLCSACSQNKAYPLKLATFDKNSVSVLIHLEK
                     DADENYFLSATFTPEQGFHLYSKDLPITGANGLGRPTLIEAGVESQYQVTGKLLESVP
                     AEIPEFEPKDLLVYPVGAVTLRLSIELPPGNGWTDDSVKITYMACSDRGCKAPVIGQE
                     IAIRIPGKETFH"
     gene            complement(945..2288)
                     /gene="glnA"
                     /locus_tag="PKI33_10635"
     CDS             complement(945..2288)
                     /gene="glnA"
                     /locus_tag="PKI33_10635"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNM37514.1"
                     /translation="MDSKELLKRVKEDNVKFLSLQFTDVLGAVKSVDMPIRHLEDVLK
                     DGAWFDGSSVEGFARIQESDMRLKVDPETYVLLPWSSPESRRARVFCDIYTPSGEPFE
                     GDPRGALKRILKKIDERGWKFNIGPEPEFFLFKGNDGSGNVHPVPHDTGGYFDFSSFD
                     EAVVVRTALMDALDQMGLDVEVGHHEVALGQHEIDFRFADALKAADNVLTLKYTVKAI
                     AAQHGLVASFMPKPVYGINGSGMHCHQSLFDTKTGNNLFFDAKDEAHLSQLAYSFIAG
                     QLEHARALAGVVAPTVNSYKRLVPGYEAPVYIGWAQQNRSALIRIPRYTEGRDKAVRA
                     ELRFPDPSSNPYLAFTVMLAAALDGIDKNMKAPKPLNNINLYHLNKEERAKLGVTELP
                     GSLAESLKELEKNEVLKAALGDYLFEAYMRAKWEEWDEFRLRVTDFEIQRYLETA"
BASE COUNT          617 a          565 c          547 g          621 t
ORIGIN      
        1 cctgacaagg tgggcaccag gtcgcatcaa actcaacgac aggcatctga cccagcgctt
       61 gtgctttttt actttcgctt gccagcatcg ccgctaagtc tccatcctct ggcttgagcc
      121 gcacaatggt aaaccgagta gacgatgtta cagcagtttc ttccgatggt gcgtctgtga
      181 tttgaacaat cacagacggc ggttcatctg tagcgactgg agtctctact tcttgtggat
      241 ttggcgttag tgctttacat gccaatactc ctagaagagc aatcaatatc aaactatatt
      301 ttttcataag tctcccatct tttatagaat tagtgaaacg tctctttgcc ggggatacga
      361 attgcaatct cttgaccaat aaccggcgct ttacagccac ggtctgaaca agccatgtag
      421 gtgattttca ctgaatcgtc ggtccaacca tttccgggtg gaagctcaat ggacagacgc
      481 aaggtgactg ccccaacagg ataaaccaaa agatccttcg gctcaaattc tggaatttcg
      541 gctggtacac tttcaagtaa tttccccgtg acttgatact gactttcaac gccagcttct
      601 attaatgttg gacgtccaag tccatttgcc cctgtgatgg gtaggtcttt actatataga
      661 tgaaatcctt gctctggggt aaacgtagca gaaaggaaat aattttcgtc cgcgtctttt
      721 tccaagtgga ttaagacgct taccgaattt ttgtcgaaag ttgccaattt aagcggatat
      781 gctttatttt gagaacatgc actgcataat agaacactta agatagcaaa agcaaaaatc
      841 tttttcattg aaaataatta taagacaaag tgaatttcaa ggaataaaac aaaacggcac
      901 tcagaatttt ctgagtgccg ttttgttttg gctagtcaga acgtttatgc tgtttctaaa
      961 tagcgctgaa tttcaaaatc tgtcacgcgt aggcggaatt catcccattc ctcccacttg
     1021 gcacgcatat aggcttcaaa cagatagtct ccaagagctg ccttcaagac ttcgttcttc
     1081 tctaattcct tcaaagattc agccaacgag ccagggagtt cggtcacacc gagcttggcg
     1141 cgttcttctt tgttcaggtg atagagattg atgttgttta gtggtttagg cgccttcatg
     1201 ttcttgtcga taccatcgag cgcagcggca agcatgacgg tgaacgccaa atacgggttg
     1261 gacgaaggat cagggaagcg caattccgcg cggacggctt tatcgcgacc ttctgtgtag
     1321 cgcggaatac ggatgagtgc cgagcggttc tgctgagccc aaccgatgta cacaggcgct
     1381 tcataacccg gaactaaacg cttgtatgag ttcacggtcg gagcgacgac gccagcaagg
     1441 gcgcgggcat gttcgagttg accggcgatg aacgaatacg ctaactgcga gagatgagct
     1501 tcatccttgg catcgaagaa caaattattg ccggtcttag tgtcgaacaa tgattgatgg
     1561 cagtgcatac cagaaccgtt gatgccataa acaggcttgg gcatgaacga agcgacgagt
     1621 ccgtgttggg cggcgatcgc cttcaccgtg tacttaagcg tcaggacgtt atctgcagct
     1681 ttgagcgcgt ctgcgaagcg gaagtcaatt tcatgctgac ccaacgccac ttcgtgatgc
     1741 ccaacttcca catcgagtcc catttgatcg agagcatcca tcaaagcggt gcgcacaaca
     1801 acggcttcat caaatgaaga gaaatcaaaa taaccaccag tatcgtgggg gacggggtga
     1861 acattaccgc tgccatcgtt gcccttgaac aggaagaact ctggctcagg accaatattg
     1921 aacttccaac cgcgttcatc aatctttttg agaatacgct tcaatgcgcc gcgtgggtcg
     1981 ccttcaaacg gttcgcctga tggagtatag atatcgcaga acacacgcgc gcggcgagac
     2041 tcaggggaag accatggcag caacacatag gtttcagggt ctactttgag tcgcatgtcg
     2101 ctttcttgaa tacgagcaaa gccttctaca gaagaaccgt cgaaccaagc accatctttc
     2161 aacacatctt cgaggtggcg aatgggcata tccacacttt tgaccgcccc gagcacatct
     2221 gtaaattgga gcgaaagaaa tttgacattg tcttctttca cccgcttgag caactctttt
     2281 gaatccatgt tcatctcctt tttgattttg cttacacagt ttttgatact gaccaacagc
     2341 ttcaagtgta
//
LOCUS       DAOREF010000508         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_33943_length_2350_cov_2.688453, whole genome shotgun sequence.
ACCESSION   DAOREF010000508 DAOREF010000000
VERSION     DAOREF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33943_length_2350_cov_2.688453"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="mmuM"
                     /locus_tag="PKI33_10640"
     CDS             <1..622
                     /gene="mmuM"
                     /locus_tag="PKI33_10640"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HNM37515.1"
                     /translation="KEENRVGRVRPLIAGSVGPYGAYLADGSEYRGDYNLSEDELIAF
                     HRPRVEALIASGADLLACETIPCGIEARALCRLLAEFPNTVAWFTFTAKDGELISNGE
                     RIADIAAFLDSQPQAAAVGINCTSPIHIPSLVREIKKNTSKPVIVYPNSGEYYSAVTN
                     TWHGETSGDKFGLQSKEWFENGATLIGGCCRTTPGHIHEVSNWARK"
     gene            730..1794
                     /locus_tag="PKI33_10645"
     CDS             730..1794
                     /locus_tag="PKI33_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM37516.1"
                     /translation="MKKTLVVFLAFLVAITACTEATPMPTHTPIPVTDTPSQTSTLEP
                     TATFTPAPTATPVPHPMSIIALRNQEYPGSEITVVKELDRGINYRRYYVYYLSEGLKI
                     YALLTIPNGEKPEEGFPAIVFNHGYIPPDVYKTTERYIAYVDQLARAGYVVFRIDYRG
                     HDQSEGEATGAYGDPGYQVDVMNALASLKQHPDVNPEKIGMWGHSMGGYLTLRAMVIS
                     NDVKAGVIWAGVVASYSDLLYNWRRTGAFTPSPSSTGRGWRSTWIEQYGTPEQNPVFW
                     DSISATSYLADLSGPLELHHGTADEDVPVEFSIRLAELGRQYGQIVNLYTYEGDNHNI
                     SNYFTQAMNQTITFFDIYLK"
     gene            complement(1853..>2350)
                     /locus_tag="PKI33_10650"
     CDS             complement(1853..>2350)
                     /locus_tag="PKI33_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNM37517.1"
                     /translation="SIIVMGWRGKRTFRESVLGSVLDEVIWGSDTPVVVGKLSLPLNS
                     MQRVIFIVPAKAVPPIALRRMLEANFAIAKALNVPLLVRADKSYVQTLEALFVAIHPE
                     QEYKLEVLKDQLKPEHLEHEAVSAFIILPGFGSRKRVQDTLGNIPEQVAKHFDGNLAI
                     LHFDK"
BASE COUNT          613 a          602 c          565 g          570 t
ORIGIN      
        1 aaaggaagag aatcgcgtgg gtcgtgtccg tccattgatt gccggttctg tgggtcccta
       61 tggcgcgtat ctcgctgacg ggtctgaata ccgtggtgat tacaatttga gcgaggatga
      121 gctgatcgct tttcatcgtc cgcgagtgga agcgctcatc gcctcgggcg cggacctgct
      181 tgcctgcgaa accatcccat gcggaatcga agcccgagcc ctgtgccgtc tccttgctga
      241 gttcccaaac acagttgcct ggttcacctt caccgctaaa gacggtgaac tcatcagcaa
      301 cggcgagcgc attgctgaca ttgctgcttt tcttgatagt caacctcaag ccgctgcggt
      361 tggcatcaac tgtacctcgc ctatccatat cccttctttg gtgcgcgaga tcaaaaagaa
      421 cacaagcaag ccagtcattg tatatccaaa ttctggtgag tactatagtg ccgtcaccaa
      481 cacatggcac ggtgaaactt ccggtgacaa gtttggtttg cagtcgaaag aatggttcga
      541 gaacggtgct actctcattg gcggatgctg ccgcaccaca ccgggtcaca ttcacgaagt
      601 gagcaattgg gcaagaaaat aaaattcttt cttaactttt actgatacaa accatgtata
      661 aagagacggt aatggaccgt ctcttttttg ttctacatca cgttgagtga aaaatttaaa
      721 ggagaaagaa tgaagaaaac tttagtggta ttcctcgcgt ttttggtggc gatcacagcc
      781 tgcaccgaag cgactcccat gcctacccat acgccaatcc cagtaacgga tacgccctcg
      841 caaacctcca cactcgagcc cacagctaca ttcacaccag ctccaacagc cacgcccgtt
      901 ccacatccca tgagcatcat cgcgttgcga aatcaggaat atcccggcag cgaaatcacc
      961 gtcgtcaaag agctagaccg cggcattaat tatcgccgat attatgttta ctatctttca
     1021 gaagggttga agatttacgc cttgctcacc attccgaatg gtgaaaaacc tgaagaaggg
     1081 ttccctgcca tcgtcttcaa tcatggttac attccgccag atgtatacaa aacaacagag
     1141 cgctatattg catatgtcga tcaactcgct cgggcagggt atgtggtctt ccgcatcgat
     1201 tatcgcgggc acgatcagtc cgaaggggaa gccactggtg cctatggcga ccccggctac
     1261 caagtagatg tgatgaatgc cctcgcctct ctcaaacagc accccgatgt aaaccctgag
     1321 aagatcggta tgtgggggca ttccatgggc ggatatttaa ccctgcgcgc catggtcatc
     1381 tcgaatgatg taaaagccgg tgtgatctgg gcgggggtcg tcgcttcata ctcagacctg
     1441 ttgtataact ggcggagaac aggtgctttc accccgtcac ctagttctac cgggcgtggg
     1501 tggcgttcaa catggatcga acagtatgga acgcctgaac aaaaccccgt cttttgggac
     1561 tcgatctctg ccacatccta ccttgcggat ctttctggtc cgctggaatt gcaccacggc
     1621 accgcagatg aagatgtgcc agtggagttt tcgattcgct tggcagagtt gggtagacaa
     1681 tacgggcaga tcgttaattt atatacatat gaaggcgata atcacaacat ttcaaattat
     1741 ttcactcaag ctatgaatca gaccattacg ttctttgata tctatttgaa gtaaaaaata
     1801 acgataggtc gccgaccgta gactatggtc ggcgacctat cgtcaaaagc aactacttat
     1861 cgaaatgcaa gatcgctaaa tttccgtcaa aatgtttggc aacttgttcg gggatattcc
     1921 caagtgtgtc ttgaacgcgc ttgcgggagc caaaaccagg caaaatgata aaggcactga
     1981 cagcttcgtg ttccaagtgt tcaggtttaa gttggtcttt caaaacctca agtttatatt
     2041 cttgttcagg gtggatagca acaaacaaag cctctaaggt ctgcacatag ctcttgtccg
     2101 cacgaacgag caaggggaca ttgagcgcct tggcaatggc aaaattggct tccaacatgc
     2161 ggcgtaatgc aatcggcggg acagctttgg caggcacaat aaagatcacg cgttgcatgc
     2221 tattcaacgg cagggataat tttccaacca ctacgggcgt atccgaaccc cagatgactt
     2281 catccagaac ggaaccaaga acgctctcac ggaaggtgcg cttgccgcgc caacccataa
     2341 cgatgatgga
//
LOCUS       DAOREF010000509         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34013_length_2347_cov_3.122164, whole genome shotgun sequence.
ACCESSION   DAOREF010000509 DAOREF010000000
VERSION     DAOREF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34013_length_2347_cov_3.122164"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..889)
                     /locus_tag="PKI33_10655"
     CDS             complement(197..889)
                     /locus_tag="PKI33_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM37518.1"
                     /translation="MPFTFHRIPVGICNSFLLRGDRTILIDGGAMGGFPAFQRQIKAL
                     NVDPHEIELILLTHGHWDHITCLADIQKLTGAKIAIHGRDQFMVETGEPPFPDGATPY
                     GNAMSWLSKRVLHPHLPPLKVDTVILDAGMSLKEFGIPGEVIYTPGHSMGHVSILLET
                     GDALVGDMAMNDWYLRWTPGLPVLADNIEMVVDSWKKILPMGIKRVYPAHGKDFDIEV
                     MKKEIATFKPTK"
     gene            complement(893..1870)
                     /gene="galE"
                     /locus_tag="PKI33_10660"
     CDS             complement(893..1870)
                     /gene="galE"
                     /locus_tag="PKI33_10660"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNM37519.1"
                     /translation="MNIFVTGGAGYIGSSTAEALIKAGHSVTVYDSLITGHREAVPAG
                     ANFIHASLDDRTALTEALNAQKYDAVMHFAAFIEAGESMKDPGRFFLNNFSNSVQLMD
                     IAVQAGVRKMVFSSTAAVYQSSEEPLTEDSPIGPTNVYGHTKLMTEQALEWYRSIFGM
                     HYAILRYFNACGALPGRGEAHQPESHLIPRVLQIALGQAESATIFGTDYPTPDGTCIR
                     DYIHIADLVSAHILALGALEKRDKMIFNVGSGNGFSVREVIEAARKVTGHAIPAIEQP
                     RRAGDSARLVASPERIKKELGWEPKNTNLQDILSSAWEWHISHPNGYGK"
     gene            complement(1921..>2347)
                     /locus_tag="PKI33_10665"
     CDS             complement(1921..>2347)
                     /locus_tag="PKI33_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNM37520.1"
                     /translation="IGSLIPYIDATYRTIPDRDHRAIGGLSRGGGWAAELGLQNPGMF
                     ISIGLHSPAIFKGDGPAMQKLVQSIPDESRPRMWIDMGDTDREWESILEFEDVLTYFG
                     YPHEYQRYLGDHSEVYWGAHVHDYLKWYAEVWNETPEGE"
BASE COUNT          603 a          594 c          555 g          595 t
ORIGIN      
        1 gaatatgttg atgaccccgg catgatcgat tcacagatca tttccctgct caaacaaggt
       61 aacaaaatcg aagcgattaa agtctatcgt gacttaacca acgccgacct gttagatgcc
      121 aaacgagcgg tcgaaaaaat agaagcgagc attttataaa tacgaacgcc agagattttt
      181 ctctggcgtt tttgattcat tttgttggct tgaacgtggc gatctctttt ttcatcactt
      241 caatatcaaa gtccttgcca tgtgcggggt agacccgttt gatgcccatt ggcagaatct
      301 tcttccaact atccacgacc atttcaatat tatccgcaag aacgggcagt ccgggcgtcc
      361 accgcagata ccaatcgttc atggccatgt cccctaccaa agcatcaccc gtttccagca
      421 agatacttac atgtcccatt gaatgccctg gggtgtatat cacttcgccg ggaatgccga
      481 actctttcag tgacatgccg gcatccaaaa tcaccgtatc tactttgagc ggggggagat
      541 gcggatgtag tacccgcttc gaaagccacg acatggcgtt tccatagggt gtcgcaccat
      601 ctggaaatgg cggctcgccc gtctcgacca tgaattgatc ccgtccatga atcgcgatct
      661 tcgctccggt cagtttctga atatccgcaa ggcaagtgat gtgatcccaa tgcccgtgcg
      721 taagcagaat taattcgatt tcgtgtgggt caacattcaa cgccttgatt tgacgttgaa
      781 atgcagggaa acctcccatc gctccgccat cgatcagaat ggtacggtcg cctcgcaaaa
      841 ggaaagaatt gcatattcca accggaatgc gatgaaatgt aaatggcatt ttttattttc
      901 cgtatccatt cgggtgcgat atatgccatt cccaggcgct ggaaaggata tcttgcaagt
      961 tggtgttctt cggttcccaa cctaattctt ttttgattct ctcgggcgaa gcgaccaatc
     1021 tggcagagtc gcctgcacgg cgcggctgtt caatggcggg gatcgcatga cccgtcactt
     1081 tgcgagccgc ttcaatgacc tcacgaacag agaaaccatt gccgctgcct acattgaaga
     1141 tcatcttgtc acgtttttcc aatgcaccta aggcaagaat gtgcgcagaa accaagtctg
     1201 caatatggat gtaatcgcga atacaggttc cgtcgggtgt ggggtaatcg gtgccaaaga
     1261 tcgtagcaga ttcggcttgt cctaatgcaa tttgcaacac gcgtggaatc aaatgtgact
     1321 ctggttgatg tgcttcacca cgcccgggca gagcgccaca ggcattgaag taacgcagaa
     1381 tggcataatg cattccgaaa atggagcgat accattcaag cgcttgctcg gtcattaact
     1441 tggtatgacc atagacgttg gtcggtccga tgggtgagtc ttcggtcaac ggctcttcgc
     1501 tggattgata cactgcggct gtggacgaaa agaccatctt acgcacaccg gcttgtacag
     1561 caatatccat caactgcact gaattgctaa agttattaag gaagaaccgt cccgggtctt
     1621 tcatgctttc gccagcttca ataaaagcag caaagtgcat cacggcatca tatttttggg
     1681 cgttcaaggc ttcggtcagt gctgtgcgat catccaacga tgcgtggata aagttcgccc
     1741 cagcagggac agcttcgcga tgcccggtga tcaacgagtc ataaacggtg acggaatgtc
     1801 ccgctttaat aagtgcttcg gcagtagagg aaccaatata gcccgcgccg cctgtaacaa
     1861 agatattcat ttatgctcct atatcaaata gacgtttcaa aattatacct gtgacgaatc
     1921 ttattcgcct tctggggttt cgttccacac ttcggcgtac catttcaaat aatcatggac
     1981 atgtgccccc cagtacacct cagaatggtc accaagatag cgttgatatt catgaggata
     2041 gccaaaatac gtcagcacat cttcgaattc aagaatactc tcccattccc gatctgtatc
     2101 acccatatcg atccacatac gtgggcggga ttcatcagga atgctttgaa ctagtttctg
     2161 catggcaggt ccatcgcctt tgaaaattgc cggggaatgt aacccaatcg aaatgaacat
     2221 gccgggattc tgtagcccaa gctcggctgc ccaaccgcca ccacgcgaaa gtccgccaat
     2281 ggcgcgatgg tcacggtctg gaatggtgcg gtaggtggca tcaatatatg ggatgagtga
     2341 accgatc
//
LOCUS       DAOREF010000510         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34035_length_2346_cov_3.591008, whole genome shotgun sequence.
ACCESSION   DAOREF010000510 DAOREF010000000
VERSION     DAOREF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34035_length_2346_cov_3.591008"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKI33_10670"
     CDS             complement(<1..376)
                     /locus_tag="PKI33_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37521.1"
                     /translation="MKNQWLSRLSLLIIAILIFAAAGRVFTTDAMAALTINSKTLTPN
                     KAIANWPSGVPAGEKRPVIVFLPGWGGVGGVNATISAQNTNLVNQGYVTLAIGFDSSE
                     TWVSDIEQKTLDGLNKLCADTTI"
     gene            complement(539..1342)
                     /locus_tag="PKI33_10675"
     CDS             complement(539..1342)
                     /locus_tag="PKI33_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37522.1"
                     /translation="MNITGHILIIDDEASIRKTLARILQQAGFEVTTAEGAEQGLDYL
                     RTTQFDLILTDLRMPGMHGMEALKVIHTEHPTLPVILFTAQPDVNSAVEALRHGATDY
                     LLKPIKPEAIIERARNILEKQQQEKRRREIMLQIASLQNELKELDGGEPGKTQPIAPV
                     SFEKERFLKRGKLVLDLHARRVLLNDEPLNTPPTSFDYLLVLARHAPDVVDYQTLVAE
                     AQGYQTDLREAQELTKWHIHQLRQIIEADARNPVHLINVRGTGYRLVAD"
     gene            complement(1339..>2346)
                     /locus_tag="PKI33_10680"
     CDS             complement(1339..>2346)
                     /locus_tag="PKI33_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM37523.1"
                     /translation="SGIVGYAAETGTPFFTNNVDGVMFYLANPLLPDTKSEMAIPVLN
                     GDKLYGILDIQQTSARQFTQRDQQLVLTVADQLAVALHKTELYENLQTALQQEKAVRN
                     QLVQNERLAVMGRLLASVSHELNNPLQAIQNALFLLKEEKGLSQQGLNDLEIVLAESE
                     RMASLIERLRETYRPPQVEDLQPAHVNNIIEDVHALLATHLRKNSVAFEFHPDPDIPA
                     IMALPGQIRQVTLNLFMNAVEAMTDGGKLTVSTMGLNETQEIMIAVSDTGPGIAPSIL
                     PNIFDPFVTNKKRGTGIGLTISHDIVIKHRGRITAENNPETGATFKVWLPIEPASAEL
                     E"
BASE COUNT          541 a          525 c          605 g          675 t
ORIGIN      
        1 gaatagttgt atctgcacaa agtttattta acccgtccag agtcttttgc tcaatgtcac
       61 tgacccaggt ttctgatgag tcaaacccaa tggcgagtgt tacatagccc tgattaacca
      121 aattcgtgtt ctgcgccgag atcgtagcat tgacgccgcc caccccgccc cagccgggca
      181 aaaatactat caccggacgt ttttcgccag ctggaacgcc agaaggccaa ttggcaattg
      241 ctttattggg tgtcagggtt ttggagttaa ttgtcaacgc tgccatagca tcggtggtaa
      301 agacccgccc tgctgctgca aatatcaata tagcaataat taacaacgat aatcgggaaa
      361 gccattggtt tttcatgatc tctctctacc tttatttttg ttggattaag tatagcaatg
      421 atgactggtt tttctacccc gcccaaaact cctattgcag gataacagaa cccgcctcag
      481 gcgtggaccg gaggcgggtt tctatccatt gagtgagggc taccccgaac tatatcccct
      541 aatctgcaac tagtcggtac cccgtcccgc gaacattgat caaatgtacc gggttgcgcg
      601 catcggcttc aatgatctgg cgcaattgat gaatgtgcca tttggtgagt tcttgcgctt
      661 cgcgtaggtc ggtttgatag ccctgtgcct cagcaacaag ggtctggtaa tcgaccacat
      721 ctggcgcatg gcgagcgagg acaagtaaat aatcgaaaga ggtgggtggg gtgttgagcg
      781 gctcatcgtt aaggagcaca cggcgggcgt gtaggtctaa caccagtttg ccgcgtttga
      841 ggaaacgttc cttctcaaaa gaaaccggcg ctatgggttg ggttttgccc ggttcgccgc
      901 catctaattc ttttaactca ttttgcaagg aagcaatttg gagcataatt tctctgcgcc
      961 gcttttcttg ttgttgcttt tctaagatat tgcgtgctcg ctcaatgatg gcttcaggtt
     1021 tgatgggttt tagcaggtaa tctgtagcac catgacgcaa agcttcgacc gccgagttca
     1081 catcgggctg agccgtgaag agaataaccg gcaacgttgg gtgttctgtg tggatgacct
     1141 tgagagcttc catgccgtgc attcctggca tgcgtaggtc ggttaggatc aagtcaaatt
     1201 gtgtggtgcg cagatagtca agcccttgtt cggcgccttc agcagtggtc acttcaaacc
     1261 cggcttgttg caagatgcga gccaaggttt tgcggatcga tgcttcatca tcaatgatga
     1321 gaatatgacc ggtgatgttc attctaactc cgctgaggca ggttcaattg gtaaccaaac
     1381 tttgaaggtc gcgccagttt cggggttgtt ctcggcggtg atacgtcctc tgtgcttgat
     1441 aacaatatcg tgtgagatcg taagcccaat gcctgtgccg cgttttttgt ttgtaacgaa
     1501 ggggtcaaaa atgtttggca ggatgcttgg cgcgatacca ggaccagtat cagatacagc
     1561 gatcatgatc tcttgtgtct catttaaacc catcgtgctg actgtaagtt tgccaccatc
     1621 tgtcattgcc tctacagcat tcatgaaaag atttaatgta acttgccgta tctgtcctgg
     1681 cagtgccatg atagctggaa tatctgggtc tgggtgaaac tcaaatgcca cactgttctt
     1741 gcgtaggtgt gtagcaagca aggcatgtac atcttcaata atattgttga catgcgctgg
     1801 ctgtaagtct tccacttgag gcggacgata ggtttcccta aggcgttcga tcaggcttgc
     1861 catgcgttct gattcagcca atacgatttc aaggtcattt aaaccttgtt gtgaaagtcc
     1921 cttttcctct tttaggagga atagggcatt ttgtatagct tgcagtgggt tattcaattc
     1981 atgagaaacg gaggcaagca accgtcccat cacggcgagg cgttcatttt gtacaagctg
     2041 attgcgaacg gctttttctt gctgcaatgc tgtttgcagg ttttcgtata gttccgtttt
     2101 atgtaatgcg actgcaagtt ggtctgctac ggtcaataca agttgttggt cgcgttgtgt
     2161 gaattgacgt gcagatgttt gttggatatc caaaatgcca tataacttgt cgccattgag
     2221 tacagggatt gccatttcag atttggtgtc tggcaagagg gggttggcaa ggtagaacat
     2281 gacaccatct acgttgtttg tgaagaaggg cgtacccgtt tcggcggcat agcccacaat
     2341 gccaga
//
LOCUS       DAOREF010000511         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34040_length_2346_cov_2.752946, whole genome shotgun sequence.
ACCESSION   DAOREF010000511 DAOREF010000000
VERSION     DAOREF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34040_length_2346_cov_2.752946"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKI33_10685"
     CDS             <1..797
                     /locus_tag="PKI33_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37524.1"
                     /translation="SYIFLAASLLLFYVLLLKEKPLPPLIAASLITIGITYTNFAQNV
                     IALFAARPNIKQMFRFVTSVLVFLVLFTLLNNVLYPDSNPLFFIPSTLQAEQANFFPL
                     NSLRIVGLIRGFAFHNIIAPTPIFYDKDIPFIQFRFFKPEIEEISRYDLPIQNITVLF
                     WLALLIIGAILFLLTIKKSQHLRLSLALIGGIFLNVMLHLRYGKELFLYSQNWTYALV
                     LLLALSWRILGDKRWFQVVLLIFLGLLIWNNGILLHTILQVLASQV"
     gene            881..>2346
                     /locus_tag="PKI33_10690"
     CDS             881..>2346
                     /locus_tag="PKI33_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37525.1"
                     /translation="MNKRLLLAWGLGILPTYSAAGFLDSYYKTGVEILFVSLALQSLL
                     TIFIYLLLGKLILKLQQNRVDAIFSVAIALAVLAFFIGLFNMASRFPNEFDILSFVLT
                     PTALLPFGAGVLVAFPASVGFLSVARKFNFEQNRFYKFVDANLSGIVIALLFFAIYFM
                     LVTIFNQPSFDFDDIYFDTDSALYRYRFGTEFYKDYYDRTVHPYVLIIVRPLVWIVSF
                     FLKGDMLYGAFIVTALTGALCVFLVWYFVREYTSNSLYALLIAGLFGASTSQLAFGSL
                     IENYIFLGAAALIFMVLLLKDQPMYLLVIAGLTSFGITISNIAQTVIAHFFVKRNIPQ
                     LIKYGIIVGALIIPLNLLNNFIYPDAHPYLWEVSTLQFEEKNVFQPTILRANYLSRVM
                     VMHSFVAPDPLFIKEDIPFVKLWLFRAVQKQNLLEIARYETTLSKWLVSIWLLFVAIG
                     GVLFLKNLFKQDNRFSFAFIFIVLFSFVLHMRYGRDVF"
BASE COUNT          570 a          479 c          437 g          860 t
ORIGIN      
        1 aatcttacat tttcctcgct gcgagtttgt tgctgttcta cgtcttgctc ctgaaagaga
       61 agcctttacc gccgctaatt gccgccagtt tgataacgat cggcattaca tatacaaact
      121 ttgcacagaa tgtgattgca ttatttgccg cacgcccgaa tattaagcag atgtttcgtt
      181 ttgtgacttc agtcttggta tttcttgttt tgtttacact gcttaataat gtgctctatc
      241 cagattcgaa tccgcttttc tttattcctt ctacattaca ggctgaacag gcaaatttct
      301 tccctctgaa ctcgctgcgt atcgtagggc tgatacgagg ttttgctttc cataacatta
      361 ttgcacctac tccaattttt tatgataaag atatcccgtt tattcagttt cgtttcttca
      421 aaccagaaat tgaagaaatc agccggtatg accttccaat tcaaaacatc acagttttgt
      481 tttggttggc gcttttgata attggtgcta ttctcttttt gcttacaata aaaaaaagtc
      541 aacatttgcg attatctttg gcattaatag gcgggatttt tctaaatgtc atgttgcact
      601 tgcgttatgg taaggagtta ttcttatatt cccaaaattg gacatatgct ctcgtattgc
      661 ttttagcctt gtcatggaga atattagggg ataagagatg gttccaagtt gttctgctaa
      721 tatttttggg attattgata tggaataatg gaatactact tcatactatt ctacaagttc
      781 ttgcatctca agtatgagtt tctcctatac tatttgaaat ctatgcctgt ggtaaactcc
      841 aaaacgctct atctggaatg gaacgagatt aatgcagact ttgaataaac gacttctact
      901 agcctgggga ttgggtattc tacccactta tagtgcagca gggtttctgg attcatatta
      961 caaaactggg gtggaaattt tattcgtttc tttagctctt caatctttac tcactatatt
     1021 catttattta ttgcttggaa agttgatatt gaaattgcaa caaaaccgag tcgatgcaat
     1081 cttttctgtc gcaattgctc ttgctgtgct cgcttttttt attggactgt tcaacatggc
     1141 aagccggttt cctaatgaat ttgatattct gtcctttgtt ttgacaccaa cagcattact
     1201 gccttttggg gctggagttt tagttgcatt tcctgcctct gttggtttct tgtctgttgc
     1261 acgaaaattt aattttgagc aaaatcgttt ttataaattt gtagatgcga atttaagtgg
     1321 aattgtaatt gcactactct tctttgcaat ttatttcatg ctcgtaacca ttttcaacca
     1381 acccagcttt gactttgatg atatttactt cgacaccgac agcgcgctgt atcgctatcg
     1441 ctttggcaca gaattttaca aagattacta tgatcgcaca gttcaccctt atgtgttgat
     1501 catagtccgc ccattggtgt ggatagtctc tttctttctc aaaggtgata tgctctatgg
     1561 cgcattcatc gttacagcat tgaccggcgc tctgtgtgtc tttctcgttt ggtatttcgt
     1621 acgagaatac acttcgaact cactttatgc tttgttaatt gcgggattat tcggcgcttc
     1681 tacatctcaa ctcgcatttg gctctctgat cgagaactat atcttcctcg gcgcagcggc
     1741 tttgattttc atggtcttgc tgttaaaaga ccagccgatg tatcttctcg taattgccgg
     1801 gttgacctcg ttcggtatta ccatctccaa tattgcacag acagtgattg cgcatttctt
     1861 tgttaagcgt aatatcccac aactgataaa gtacggcata atcgtaggcg ctttaatcat
     1921 tccattgaat ttgctcaata actttattta ccctgacgca catccctacc tttgggaagt
     1981 atccactctg caatttgaag aaaaaaatgt attccaaccg actatcctgc gcgccaatta
     2041 tctgagccgt gtgatggtga tgcacagttt cgttgcgcca gatccattat ttatcaaaga
     2101 agatatccca tttgtgaaac tttggctttt ccgtgcggtc caaaaacaaa acctattaga
     2161 gattgctcga tatgaaacta cactgagtaa atggcttgta tccatttggc ttttgtttgt
     2221 agccataggc ggagtacttt ttctcaagaa tcttttcaag caagataatc gtttttcctt
     2281 cgcttttatt tttattgtct tgttctcttt tgtgctccac atgcgttatg ggcgtgatgt
     2341 gttctt
//
LOCUS       DAOREF010000512         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34134_length_2343_cov_3.182692, whole genome shotgun sequence.
ACCESSION   DAOREF010000512 DAOREF010000000
VERSION     DAOREF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34134_length_2343_cov_3.182692"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKI33_10695"
     CDS             <1..307
                     /locus_tag="PKI33_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNM37526.1"
                     /translation="KEWGHVVVLKGAFTVIASPSGYRTIIPVATAALARAGTGDVLAG
                     LIVGLRAQGVPAYEAAVAGAYIHAQAGLRAAEELGTTISVLASDILASISKIIAELE"
     gene            complement(344..>2343)
                     /locus_tag="PKI33_10700"
     CDS             complement(344..>2343)
                     /locus_tag="PKI33_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37527.1"
                     /translation="KTQNAHSTSIPEPSSPSISQKKEKQINFEDVQKPSIVLTVTKDG
                     FKMGLIGGVLGVIFAYIFRFLFDSPPLAETTIFGACISANITSGIIFFRRGIGANLSV
                     SFGGFVSGFVSGAITSLGYGIVSLVAPILLYLAFCGLFTPLLFAVLGSIIIWIPRLFI
                     NPTPENYKENFTPNKQVSRETGKSTYTDTLIMNAPPVAKTIIEDQLIATPVVQENAVN
                     PTDSPKFWIYPTRRNVFVFSLTESDIHIASGPIESLQKVKNSILQSKSTEQFLKDKEN
                     IPYKNITKVRQILSTIRIYYTNGSQETSRALDCKDTKMANELIDFLRNHIRSQFDETN
                     KPVGIGTSLFTSGFIMLVVLAGTAFFYYGAYELEAGIISPRGSARTRAILNLLDLLGT
                     NGVLVIGTILFILSLGLMLYLIKNPPTVTEFIKKSEQKIEEATPAPPLQPELPKNTVL
                     SKPEQVIPVTPQAKTGAILIDGLLSGLILGVIGMILPGISIFTGEKQLAQWGMYLDGF
                     LMLFSGVLTGILFQGRDIGKSQSTIFGGAIAGALTGLLSGVILGLSAQNETITNMATQ
                     DGLKIYIMVCMVSPSIGGAFVGAFSAWMFGLTSKESAMPESNFPLTQKTAGTQANADP
                     TQDESITRRFGKILKRRLILAAILGVIVFVCALCQSLIGSL"
BASE COUNT          667 a          467 c          512 g          697 t
ORIGIN      
        1 aaaggaatgg gggcatgttg ttgtattaaa aggtgcattt actgtgattg cttctccgag
       61 cgggtatagg accataatcc ctgtagctac agcagccctt gcccgagcag ggacgggaga
      121 tgtgttggct ggcttaattg tcggtcttcg tgcccagggt gtgcctgcct acgaagcagc
      181 ggttgcagga gcgtatatcc atgcccaggc tggtctacgt gccgctgagg aattaggtac
      241 gactatttct gttcttgcga gtgatatatt ggcttctatt tccaaaatta ttgcagagtt
      301 ggaataacgt ttacgtgatt ttccggaagt tactttccaa ggatcataac gagcctataa
      361 gcgattgaca aagagcgcag acaaaaacaa tcacacctaa gatggcagcg agaatcaatc
      421 ttcttttgag tatcttgccg aaacgtctcg taatactttc atcttgggta ggatcagcgt
      481 ttgcttgagt gcctgctgtc ttttgtgtga gaggaaaatt actctctggc atagcacttt
      541 cttttgatgt aagaccaaac atccaggctg aaaacgctcc tacgaatgct cctccaatag
      601 atggggagac catgcaaacc attatataaa ttttgagtcc gtcttgtgtt gccatattag
      661 taattgtctc attctgtgca cttaatccca atatcacgcc tgatagcaac cccgtgagag
      721 cgcccgcgat tgcgccgcca aaaatggttg actgactctt tccaatatca cgtccctgaa
      781 acaatatccc agttaagaca ccggaaaaca gcataaggaa accatccaaa tacatacccc
      841 attgcgcaag ttgcttctcg cctgtgaaaa ttgaaattcc tggcaagatc attcctatca
      901 ctcccagaat taacccagat aacaacccat ctataagaat agctccagtt ttagcttgag
      961 gcgtaaccgg aataacctgt tctggcttgg aaagaacggt attttttgga agttccggtt
     1021 gcaacggggg ggcaggcgtc gcctcttcta ttttttgttc gctctttttg atgaattcag
     1081 tgaccgttgg tgggtttttg atcaagtaaa gcattaatcc caaagaaaga ataaagagta
     1141 ttgtacctat aaccaaaacg ccattggtac caagcaaatc taataaatta agaattgctc
     1201 tggtgcgtgc actgcctcgg ggagagatga tacctgcttc gagttcgtat gcgccataat
     1261 agaaaaaggc tgtacctgcc aaaacaacaa gcataatgaa gccagaagtg aatagcgatg
     1321 tgcctattcc gaccggcttg ttggtttcat cgaattgtga acgaatatga tttcttaaga
     1381 aatctattag ctcattcgcc atttttgtgt ctttgcagtc taacgcccgc gaagtttctt
     1441 ggcttccatt tgtataataa atacgaatag tagaaaggat ttgccgtacc tttgtaatgt
     1501 ttttataagg aatattctct ttatccttta gaaattgttc agtagattta ctttgtaaaa
     1561 tgctgttttt gaccttttga agcgattcaa ttggacccga agcaatatgt atatcacttt
     1621 cagtaagaga aaagacgaat acatttctac gagtagggta aatccaaaac tttggactat
     1681 ctgttggatt tacggcattt tcctgaacaa caggtgtggc gataagttgg tcttcaataa
     1741 tcgttttagc aacaggaggg gcgttcatta tgagagtgtc ggtgtaggta gattttccag
     1801 tttctcgaga gacctgcttg ttgggtgtga agttttcctt atagttttct ggagtagggt
     1861 tgataaataa tcttggtatc caaatgataa tactgccaag tacggcaaat aggagtggtg
     1921 tgaataatcc acaaaaagcg agatacaata aaatcggtgc tactaacgaa actattccat
     1981 accctaaaga tgtgatagct ccagatacaa atccacttac gaaacctccg aaactcacgc
     2041 taaggtttgc tccaattcct ctacggaaaa agataattcc acttgtaata tttgcggaga
     2101 tacatgcacc gaagatagtt gtttctgcaa gcggtggaga atcaaataag aatctgaata
     2161 tatacgcaaa aataactcca agtaccccgc ctatgagtcc cattttgaag ccatctttgg
     2221 ttacagtaag tacaatagaa ggtttctgca cgtcttcgaa attgatttgt ttttccttct
     2281 tttgagaaat tgaaggggaa gacggttctg gtattgaggt cgaatgcgca ttttgtgttt
     2341 ttt
//
LOCUS       DAOREF010000513         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34158_length_2342_cov_3.930039, whole genome shotgun sequence.
ACCESSION   DAOREF010000513 DAOREF010000000
VERSION     DAOREF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34158_length_2342_cov_3.930039"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKI33_10705"
     CDS             complement(<1..398)
                     /locus_tag="PKI33_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37528.1"
                     /translation="MSSNFSSSLERAILETLAYSDVFDYPLTLDELHRYLTYPATKEE
                     IMNCLNSSLSVSSNSNFYFLPKRDPLVDIRCERKTLSLPAFRRAILYGRILGMFPFVR
                     MVSMTGSLAVLNLSKDADMDYMLVIQPSRL"
     gene            complement(411..1907)
                     /locus_tag="PKI33_10710"
     CDS             complement(411..1907)
                     /locus_tag="PKI33_10710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM37529.1"
                     /translation="MFQYFLAPVVMIYTMTMLTLAIYIGNLLYLMSISLKNRNKIELV
                     TSQKLERFPVVTVQLPIYNEWYVAERLLDACAALDYPKDLFEIQVLDDSTDETAILIA
                     QKVKSIQQTGVNIVHIHRTNREGFKAGALANGLATAKGEFIAIFDADFLPQKDFLLQL
                     LPRFDHEKVAFVQARWGHLNRDYSLLTMLQSFSLDAHFAIDQLGRSCADYVFNFNGTA
                     GVWRKSAILDVGGWHADTLTEDMDLSYRTFLKGWSARYAGDVEAPAELPVSFTAYRRQ
                     QYRWARGSLECAIKYIPVIWRGDFPFARKLQATFHLTGYLLHMLTVLLMLCYPLLLVF
                     ARQFPTLLTPMGIGLLMNFLAIVPALYFIVAQHLLKRHWLRSLPTILLLSVFASGMVL
                     NTMRALYQILQKHAVPFERTPKFGITERSQSWRGNRYIIRFDALVLLEVLLGCVNLGS
                     AWMALSSGYYLMMIYAFLFALGLFFFSGFTLFQSISARFYPDPKPASA"
     gene            complement(1909..>2342)
                     /locus_tag="PKI33_10715"
     CDS             complement(1909..>2342)
                     /locus_tag="PKI33_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37530.1"
                     /translation="SGNVVQDQPMNERAYLLDAGNTLFVEHSVLGVGLGASPLAMKMR
                     FENFRINYQPPHFTPLLSALETGVVGGGFYLLLFLAPGVTFLLRWRHYIQYPLVLGAF
                     SLLIAISVVNLFDYYTWMYAPGRLWQWLAWGLYSMTLEQAG"
BASE COUNT          769 a          497 c          554 g          522 t
ORIGIN      
        1 cacaagcgac ttggttgtat gacgagcatg tagtccatgt ctgcatcttt ggagaggtta
       61 agaacggcga gagagccagt catagatacc attcgaacaa atggaaacat accaagaata
      121 cgtccgtata gaatggcgcg gcggaaagca ggcagagaca aagttttgcg ttcacaccga
      181 atatctacaa gagggtcacg tttggggaga aaataaaaat tggaatttga tgagacagat
      241 aaagaagagt tcaaacaatt cataatttct tcttttgtgg cgggataggt caagtagcga
      301 tgcaattcat ccaaggtcaa cgggtaatca aagacatccg agtacgctag cgtttcgagg
      361 atggcgcgtt caagagagga agaaaagttc gaagacaagg tcaatgcctt ttaagcagaa
      421 gcaggtttcg ggtctggata gaagcgagca gaaatggatt ggaatagagt aaagcctgag
      481 aagaaaaata atcccaacgc gaataagaag gcatagatca tcattaaata ataaccagaa
      541 gacaaagcca tccaagcgga cccaaggttg acacaaccaa gtaaaacttc aagaaggact
      601 aaagcatcga aacggatgat gtagcgattg ccacgccaag actgtgagcg ttcggtgata
      661 ccaaattttg gggtgcgttc aaatgggaca gcatgttttt gcaaaatttg gtataaggcg
      721 cgcatcgtat ttagcaccat gccagaagca aacacagaga gcaaaagaat tgttggcaaa
      781 gaccgtagcc agtgacgttt gagcaaatgt tgagccacaa taaaatacaa agccggaaca
      841 atagcaagaa agttcattag taaaccaata cccatgggcg ttaacaaggt aggaaactgc
      901 ctcgcaaaca ctagcaacag tggatagcat aacataagca agacggtaag catatgcaac
      961 aaatacccgg tgaggtggaa tgtagcttgc agtttgcgcg caaagggaaa atcgccacgc
     1021 cagatcacag ggatgtattt gatggcgcac tctaaacttc cacgcgccca gcgatattgt
     1081 tgacggcgat atgctgtgaa actgaccggc agctcagctg gtgcttccac atcccctgcg
     1141 tatcgagccg accagccttt taggaatgtc cgataggaca ggtccatatc ttctgtcaaa
     1201 gtatcagcat gccaaccgcc aacatcaaga atagcagact tacgccacac acctgctgtg
     1261 ccattgaaat taaaaacata atcggcacag gaacgtccaa gttggtctat ggcaaaatgc
     1321 gcatccagtg aaaaggattg gagcatggtt agtagggaat aatctcggtt gagatgcccc
     1381 caacgagctt ggacaaaggc aaccttttca tggtcgaacc gtggtaacag ttgcagaaga
     1441 aaatcttttt gggggagaaa atctgcatca aaaatggcaa taaattcgcc tttggcggtt
     1501 gctagtccat tggcgagagc gcctgctttg aaaccttctc ggtttgtgcg gtgtatatgg
     1561 acaatgttca cacccgtttg ctgaatgctt tttacttttt gagcgatgag aatagcggtt
     1621 tcgtctgttg agtcatccaa aacttgaatc tcgaataagt cttttggata atccagtgca
     1681 gcgcaagcat ctaataaacg ctctgccaca taccactcat tgtagattgg caattgcacc
     1741 gtaacgactg gaaacctctc cagtttttgg gaagtgacca actcaatttt gttacggttc
     1801 ttcaagctaa ttgacatcaa gtacaagagg ttgcctatgt aaatcgccaa ggtcaacatt
     1861 gtcatagtgt agatcataac cacaggagca agaaagtatt gaaacatatt agcctgcttg
     1921 ctctagtgtc atagagtata gcccccatgc cagccactgc caaagacgcc ctggggcata
     1981 catccaagtg tagtagtcaa ataaatttac aacggaaata gcaatcaata aagaaaaagc
     2041 tcccaataca agtggatatt gaatgtaatg tcgccagcgc aacaggaaag tcacaccggg
     2101 agcaagaaac aagagaagat aaaatccccc gccaactacg ccagtttcca aagccgaaag
     2161 caagggcgta aaatgcggag gttgataatt aattcgaaaa ttttcaaagc gcattttcat
     2221 cgcaagcggc gaagccccga gacctactcc aagcacagaa tgctcaacaa aaagagtgtt
     2281 gcctgcatca agcaaatagg cacgttcatt catgggctgg tcttgcacca cattccctga
     2341 at
//
LOCUS       DAOREF010000514         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34211_length_2340_cov_4.161488, whole genome shotgun sequence.
ACCESSION   DAOREF010000514 DAOREF010000000
VERSION     DAOREF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34211_length_2340_cov_4.161488"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..844)
                     /gene="mutM"
                     /locus_tag="PKI33_10720"
     CDS             complement(23..844)
                     /gene="mutM"
                     /locus_tag="PKI33_10720"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNM37531.1"
                     /translation="MPELPEVETVARSIEPDIIGRTIISADVRWARTVATPSPKKFRE
                     QVAGQRILGVSRRAKYIVIQLENYNLLIHLRMSGDLTVRKGKIHAEKHDRLILALSGK
                     RGDESYLAFNDTRKFGRVWLTDQPETVLGKLGPEPLSKEFTPQWLYENLQKKNRQLKP
                     LLLDQTFLAGVGNIYADESLHMAKLHPLRLSKSVTLKQATALHEAIRFVLKEGIRRNG
                     ASIDWVYRGGEYQNHFRVYDREGTPCLVCGTEIQKLTVGQRGTHVCPTCQPLERK"
     gene            complement(910..1899)
                     /locus_tag="PKI33_10725"
     CDS             complement(910..1899)
                     /locus_tag="PKI33_10725"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNM37532.1"
                     /translation="MHHVHHDLKVYGGIKLFGGTGSPELSQKIAEYLDLPLSGREVIE
                     FPNENLFVKLNGSVRGQDVYVIQTTSSPVHRNLMELLIMIQTLKLDSAARITAVVPYL
                     CYGRSDKKDQPRVPITARLVADMLEAAGADRYMTLDPHAGQIQGFFSVPGDVLTASHL
                     LIHHINQTLRADMKDPVVVSVDLGFAKKGRNYAADMDMPIAFIEKRRHGNDAKAEALT
                     LIGEVKDRDVIIVDDEVDTGGSIAQAVNVVMQNGARDVYLAFIHPILSANGAARLASL
                     PIKQIITTNSVPLSPEKLKILEGKITVLSIAPLLGEVIRRAHEGRSVGEMFNE"
     gene            complement(1905..>2340)
                     /locus_tag="PKI33_10730"
     CDS             complement(1905..>2340)
                     /locus_tag="PKI33_10730"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNM37533.1"
                     /translation="MSMQDEMNWFEGLSKRDSHEKPFAIEIRKGKAWKLIGNCGVFGI
                     EFTNRNAELGIMIGDKSEWNKGYGAEAMSLLQRHCFETLNLNRVYLRVYEENVPAVRA
                     YEKAGFVLEGRQRQAVYKKGKYEDVLFMSVLRSEWDTRMKEK"
BASE COUNT          584 a          614 c          533 g          609 t
ORIGIN      
        1 gtggcgaaat taagctccat ggttatttcc tttcaagcgg ctggcaggtt gggcatacat
       61 gggtgccacg ttgcccaacg gttagctttt gaatttcagt accgcaaacg aggcagggag
      121 tcccttcacg gtcgtatacc cgaaaatgat tttgatactc accgccgcga tagacccaat
      181 cgatgctggc accgttgcga cgaatgcctt ctttcaacac aaaccgaatc gcctcatgca
      241 aggcggttgc ctgtttcagc gtcacagact ttgacaacct caaagggtgc agctttgcca
      301 tatgcaaaga ctcatctgca tagatgttgc caaccccggc aagaaatgtc tgatcgagca
      361 ataacggctt gagttgacga ttcttcttct gcagattttc gtacaaccat tgtggagtga
      421 actccttact caggggttcg ggtccgagtt tgccgagtac ggtttcaggc tgatctgtta
      481 gccacacacg cccgaacttg cgcgtatcgt tgaaggcgag gtaactttcg tctccgcgtt
      541 taccgctcag ggcgaggatg aggcggtcat gcttttcggc atggatttta ccttttctta
      601 cggtcaggtc accgctcatc cgcaaatgta ttaggagatt gtaattttcg agctggataa
      661 cgatatattt tgcccgccgt gataccccta aaatgcgttg acctgccacc tgttccctga
      721 attttttcgg cgagggtgta gcaaccgttc gtgcccaacg cacatcggca gagatgatgg
      781 tacgcccgat gatatcgggt tcgatgcttc tggctacagt ttcaacttca gggagttcag
      841 gcataaagtc agctttcagg tatcagcttt cagaaattag ctacgaactg actactgaaa
      901 gctgttcttc tattcgttaa acatctctcc aaccgaacgc ccttcgtgtg cgcggcggat
      961 gacttctccc aacaggggag caatcgagag gacagtaatc ttgccttcga gaatttttaa
     1021 tttctcaggg gaaagcggaa ccgagttggt agtaataatc tgcttaatcg gcagggatgc
     1081 caagcgcgct gcgccatttg ccgaaagaat agggtgaatg aacgccaaat acacatcgcg
     1141 tgcgccattt tgcataacca cattcacagc ctgtgcaatc gaaccgccag tgtccacttc
     1201 atcatccaca ataatgacat cgcggtcttt cacttcaccg atcaaggtca gtgcttcggc
     1261 tttggcatca ttgccatggc ggcgcttttc aataaaagcg atgggcatgt ccatatctgc
     1321 ggcgtagtta cgccccttct tggcaaaacc caaatcgaca gagaccacga ccgggtcttt
     1381 catatccgca cgcaaagtct gattgatatg gtggattaac aagtgtgaag cggtcagcac
     1441 atcacctggc acagagaaga aaccctgaat ttgcccggca tgtgggtcga gcgtcatgta
     1501 gcgatctgca ccagcggctt cgagcatatc tgccaccaaa cgggcagtga tcggcacgcg
     1561 cggctgatct tttttatcag aacgcccata acaaagataa ggcacaaccg ctgtaatgcg
     1621 cgctgctgaa tccagcttca gagtctggat catgatgagc aattccatca agttgcgatg
     1681 aacaggagaa gacgtcgtct gaatgacgta cacatcttgc ccacgcacac tgccattcaa
     1741 tttgacgaac aagttctcat tggggaactc aattacctcg cgtccactta atggaaggtc
     1801 gaggtactct gcgatctttt gcgataattc gggcgaaccc gtcccaccga ataacttaat
     1861 ccccccgtac actttcaaat cgtgatgaac atgatgcatg tggattactt ctccttcatt
     1921 cgtgtgtccc attccgagcg caacacgctc atgaagagaa catcctcgta ttttcctttt
     1981 ttataaacag cttggcgctg tcttccttct aaaacaaaac cggctttttc ataagcacga
     2041 acggcaggca cattttcttc gtatacccgt aaatagacac ggtttaagtt caaggtctca
     2101 aaacaatgac gttgtaacaa actcatggct tctgcgccgt agcctttatt ccactccgat
     2161 ttatcgccga tcatgattcc aagctcggca ttgcgatttg taaactcaat cccaaacacg
     2221 ccgcagttcc cgatcaactt ccaagccttg cctttgcgaa tttcaatggc aaaaggtttc
     2281 tcatgcgagt cacgtttcga caagccctcg aaccaattca tttcatcctg catcgacata
//
LOCUS       DAOREF010000515         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34215_length_2340_cov_3.294092, whole genome shotgun sequence.
ACCESSION   DAOREF010000515 DAOREF010000000
VERSION     DAOREF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34215_length_2340_cov_3.294092"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1182)
                     /locus_tag="PKI33_10735"
     CDS             complement(25..1182)
                     /locus_tag="PKI33_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNM37534.1"
                     /translation="MPLSSRERVQAAIHHEIPDRVPIIIGASNATGIKMVTYRRLKKL
                     LNFEAPERYLYDFPELGTALIDEPILERLHVDVRGVLDREPQHILERNASREPDSMYV
                     NSWGGGVTKAGPDNWFPSYHPLAETHAIEDLEKYPWPDMNDPTRVAHVRAHAQQLHQE
                     NKYALMGTPWLAFPMERAYEMQRMDKFFLNMGRHPDFVVELLKKTGEMCKTLMGHFLD
                     ECGDVIDIIKIGDDLGMQSSLMLSPKMYRNYVKPIHADYISFIKSKTKAKVFFHTDGD
                     VFPLLPDFVEMGIDILNPIQTSAGKMSNLPELKRQFGKDLIFCGAIDTHRVLPFGTPE
                     EVRAEVKRIIDILGEGGGYMLASVHTILDDVPAENILAMVDAVEEFGWYKK"
     gene            complement(1270..2016)
                     /gene="gpmA"
                     /locus_tag="PKI33_10740"
     CDS             complement(1270..2016)
                     /gene="gpmA"
                     /locus_tag="PKI33_10740"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNM37535.1"
                     /translation="MYKLVLVRHGQSTWNLENRFTGWTDVDLTDLGKAEAAEAGKLLK
                     EAGYDFDIAYTSVLKRAIKTLGVIQDVMNRDWLPVIRAWQLNERHYGSLQGLNKSEMA
                     EKFGEEQVKIWRRSYDVPPPALELTDERHPKFDRRYASLTPEQLPATESLKITLERVL
                     PYWTSTLAPEIKSGKRLLVVAHGNSIRALVKYLDNISESEITELNIPTGLPLVYELDK
                     DLKPIKNYYLGDAEEAAKKAAAVANQGKAK"
     gene            2166..>2340
                     /locus_tag="PKI33_10745"
     CDS             2166..>2340
                     /locus_tag="PKI33_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37536.1"
                     /translation="MSNEDVTPVRRQYLEAKKQYPDTIVFFRLGDFYETFDEDAEITA
                     RELDIVLTSRPIGG"
BASE COUNT          639 a          538 c          525 g          638 t
ORIGIN      
        1 ggagtcttta tatttcaaat agtattattt cttataccag ccaaattcct ctacagcatc
       61 gaccatggca agaatattct cagcagggac atcatccaaa atagtgtgga cggatgccag
      121 catatagcca ccgccttcgc caaggatgtc aatgatgcgt ttcacttcgg cacggacttc
      181 ttctggtgtt ccgaatggaa ggacgcggtg ggtgtcaatc gcaccgcaga agataaggtc
      241 tttgccaaac tgccgtttaa gctctggtaa gttggacatt tttccggctg acgtttgaat
      301 tggattcaaa atatcaatgc ccatttcgac aaaatctggc aaaagtggga aaacatctcc
      361 atcagtatga aagaatactt tggcttttgt tttcgacttg ataaaagaaa tgtaatctgc
      421 atgaatgggc tttacataat tgcgatacat cttaggcgaa agcataaggc tggattgcat
      481 ccccaagtca tcgccaattt tgataatatc gatcacatcc ccacattcat caagaaaatg
      541 tcccatgagg gttttacaca tttcgccggt tttcttgaga agttctacaa caaaatcagg
      601 gtgtcgcccc atattgagga aaaacttatc catgcgctgc atctcataag cacgttccat
      661 tggaaatgca agccaagggg tgcccattag agcgtatttg ttctcttgat gaagttgctg
      721 ggcatgagct cgtacatgcg caacccgggt agggtcgttc atgtcgggcc aggggtattt
      781 ttcgaggtct tcaattgcat gtgtttcagc cagtggatga taagagggaa accaattatc
      841 gggtccggct ttggtaacgc ccccacccca cgagttgaca tacattgagt cgggctcgcg
      901 gctggcgttt cgctcaagga tatgttgcgg ctcacggtct aacacaccgc gcacatcaac
      961 gtgtaagcgt tcaagaatag gttcgtcgat cagggcagtc ccaagttctg gaaaatcata
     1021 caaataacgt tcgggggctt caaaattaag cagtttcttc aaacggcgat atgtcaccat
     1081 cttaatccca gtcgcattgc ttgcaccaat aataatggga acacgatctg gaatttcatg
     1141 atgaatggct gcttgaacac gttcacgaga tgaaagtggc ataacattct ccttcggcaa
     1201 attaaaagag gacaatgctg gttattcacc atcattgtcc tctgagttga attgtttact
     1261 tactacgtat tacttcgctt tgccctgatt ggcgaccgca gcagctttct tagcggcttc
     1321 ttcagcatcg ccaagataat agtttttgat gggtttcaaa tctttatcaa gttcatatac
     1381 caagggcaaa ccggttggga tgtttaattc cgtgatttcg ctttcggaaa tgttatcaag
     1441 gtatttcacc aacgcacgaa tggaatttcc atgggcaacg acaagcaaac gcttgccaga
     1501 tttaatctca ggcgccaagg tgcttgtcca gtaagggaga acacgctcca gcgtaatctt
     1561 aagtgactcg gtggctggca actgttcggg agtcagtgag gcgtagcgtc gatcaaattt
     1621 tggatgacgt tcgtcggtca attccaaggc tggaggcgga acatcgtatg aacgtcgcca
     1681 aatcttcact tgttcttcac caaatttttc agccatttca gatttgttca aaccttgtaa
     1741 tgaaccgtaa tgacgttcgt tcaactgcca agcgcggata accggcaacc aatcgcgatt
     1801 cataacatct tgaataacgc ccaaggtttt gatggcacgc ttgagaacag acgtataggc
     1861 gatatcaaaa tcatagcctg cttctttcaa aagcttgcct gcttcggcag cttcagcctt
     1921 gccaagatcg gtcaagtcaa catccgtcca gccggtaaaa cggttttcaa gattccaggt
     1981 actttgtcca tgacggacta aaacgagttt atacattgtg gctcctaaaa aattgcccga
     2041 aggcaaagat attttatgtt cgcgggtaga ttatagtcca actccaaggc tcaaagtgcc
     2101 aaaatgtgct ttctcgtcac aatacatgta atttgtcata acaatgggta tagtaaaatc
     2161 agggaatgtc caacgaagat gtcacgcctg tccgcaggca ataccttgaa gccaaaaaac
     2221 aataccccga taccatcgtc ttttttcggc taggcgattt ttacgaaacc ttcgacgaag
     2281 atgccgaaat cactgcccgc gagttggata tcgtcctcac ctcacgcccg attggcggcg
//
LOCUS       DAOREF010000516         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34238_length_2339_cov_4.321804, whole genome shotgun sequence.
ACCESSION   DAOREF010000516 DAOREF010000000
VERSION     DAOREF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34238_length_2339_cov_4.321804"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /gene="ffs"
                     /locus_tag="PKI33_10750"
     ncRNA           complement(<1..87)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI33_10750"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(129..1511)
                     /locus_tag="PKI33_10755"
     CDS             complement(129..1511)
                     /locus_tag="PKI33_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM37537.1"
                     /translation="MSNDIIHILLVEDEDPHAELIQRAFEDQGSEFQIHRVRSLTEAR
                     EHRRLNEPKLIIADWRLPDGESLELLPNHRDPLATPIILMTSYGNERIAVEALKSGAL
                     DYVVKSPESMLDMPHLARKALDQWKSRADRIHMQEALTESEAHFRLLAENASDMITRI
                     NTNGQMFYISPACKTILGYQPEEMLGVKVFDLINPEDQPQIRELFSQKPEDSPYTITY
                     RAKHKSGHYVWLETSARAIIDHSTDEVIEIQAASRDITERKRAEEALQLAHNQLRDAY
                     ERTIEGWVRALDLRDRETEGHTQRVTDITIRVAQQLGFSEEELSHIKRGALLHDMGKI
                     AIPDEILQKPGPLNETEWERMRQHPMYAYEMLSPIAYLNPALDIPFYHHERWNGSGYP
                     HGLKGEKIPLSARLFAIVDVWDALRSDRPYRKAIPREQVIEYLSENANILFDPKLVEV
                     FLKFVKENIP"
     gene            complement(1518..1961)
                     /locus_tag="PKI33_10760"
     CDS             complement(1518..1961)
                     /locus_tag="PKI33_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37538.1"
                     /translation="MLGEPILVMLVEDNADHAELVIRTMESHHIPTRIQHVTDGESAL
                     DYLLRRNNFSDPETSPRPHMILLDLRLPRVDGLEVLRIIKEEEDLKSIPVVILTTSEA
                     EKDVMHAYEHYVNSYLVKPVGFEDFSSLMKDLGIYWLGWNKQLRA"
     gene            2133..>2339
                     /locus_tag="PKI33_10765"
     CDS             2133..>2339
                     /locus_tag="PKI33_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNM37539.1"
                     /translation="MTESALLNDRYQLEELLGSGGMANVYRARDVVLDRPVAIKVLRK
                     EYSTNENFQKQFWLEARSAANLSHP"
BASE COUNT          576 a          531 c          582 g          650 t
ORIGIN      
        1 accggcggca cccgaagact cttccttacc gttgctatct ttcgatcctg gcggggttcg
       61 aaaggcttcg ccgcgccggg cctgcccgga caggcgcaag gatacccgat gatgaggggg
      121 gtgtcaaatc acggaatgtt ttcttttacg aatttcaaga aaacttctac tagtttgggg
      181 tcgaacagaa tattggcatt ctcactgaga tattcaatga cttgttcgcg cgggatcgct
      241 ttgcgatacg ggcggtcact ccggagcgca tcccatacat ctacaatggc gaagagacgg
      301 gcggatagcg gaattttttc accttttaat ccatgaggat aaccgcttcc gttccagcgt
      361 tcgtgatgat agaagggaat atctaaggcg gggtttaagt atgcaatggg ggagagcatc
      421 tcatacgcat acatggggtg ctgccgcatg cgttcccatt ccgtctcatt gagcggacca
      481 ggcttttgaa ggatttcatc ggggatggcg atctttccca tatcgtggag taatgcgcct
      541 cgtttaatat gggagagttc ttcttcgctg aacccaagct gttgtgcaac ccgaatcgtg
      601 atatcggtaa cacgctgtgt gtgaccttct gtttcgcggt cacgcaggtc taatgcgcgg
      661 acccaacctt caatggtgcg ttcgtaggca tctctgagtt ggttgtgtgc gagctgaagt
      721 gcttcttctg ctcgcttacg ttctgtaatg tcacgtgatg cagcttgaat ttcaatgact
      781 tcatctgtgc tgtgatcgat gatggcacgt gcggaggttt ccaaccaaac gtaatgtccg
      841 cttttgtgtt tggcacgata ggtgatggtg tatggggaat cttcaggttt ttgagaaaaa
      901 agctctctga tctgaggctg atcttcgggg ttgataaggt cgaatacttt gacccctaac
      961 atttcttcag gctgatatcc aagaatggtt ttacaagccg gtgaaatata aaacatttgc
     1021 ccgtttgtat tgatgcgagt gatcatgtca ctggcgttct cagccagcaa gcgaaagtga
     1081 gcttcactct ctgtaagggc ttcctgcatg tgaatgcggt ctgcccgaga cttccattgg
     1141 tccagtgctt ttcgtgccaa gtgaggcata tccagcattg attcagggga cttcaccaca
     1201 taatctaacg caccagactt taacgcctct accgcgatgc gttcattgcc ataacttgtc
     1261 attagaatga tgggggtagc cagcgggtcg cggtgattgg ggagcaattc caagctttcc
     1321 ccatctggga gccgccaatc tgcgatgata agtttgggtt cgttcaagcg tctatgttcg
     1381 cgggcttctg taagagaacg cacacggtga atctgaaact cagacccctg gtcttcaaaa
     1441 gcgcgctgaa tcagctccgc atgcggatct tcgtcttcaa ctaaaagaat atgaatgatg
     1501 tcattcgaca tgagggatta agcgcgtagt tgtttattcc aacccaacca gtaaatgcca
     1561 aggtctttca ttaagctgct gaaatcttca aacccaaccg gctttactag atagctgttg
     1621 acataatgtt catacgcgtg cataacgtct ttttcagctt cagacgttgt tagaataaca
     1681 acaggaatgc tcttaaggtc ttcttcttct ttgatgattc gcagtacttc caacccatca
     1741 acacggggta agcgtaagtc caaaaggatc atatgtgggc gagggctggt ttcagggtca
     1801 ctaaagttgt tgcgacgcag gaggtaatcc aatgctgatt cgccatcggt tacatgttga
     1861 atccgtgtag gaatgtgatg actctccatg gttcggatga caagttctgc atggtcagca
     1921 ttatcttcaa cgagcatgac aaggattggt tcgcctagca tggtggatct cctaaacaac
     1981 catattgtaa acgatttacg aataacgggt gaaaatcgtt atgacaactt taattaacag
     2041 acctgtaaag acaaataaac aataaaatgc ccaaaatatc gtttttttgt gtatgtgtct
     2101 ctatgtagaa ctcacggtat aatctcacca taatgaccga aagtgcatta ctaaacgacc
     2161 gatatcaact tgaagaactc ctagggtctg gcggcatggc gaacgtttat cgcgctcgcg
     2221 atgtcgtgct agaccgtccg gttgcgatta aggtcttacg caaagaatat tccaccaatg
     2281 agaactttca gaaacaattc tggctggaag cgcgttccgc tgcgaatctt tcgcacccc
//
LOCUS       DAOREF010000517         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34261_length_2338_cov_4.575558, whole genome shotgun sequence.
ACCESSION   DAOREF010000517 DAOREF010000000
VERSION     DAOREF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34261_length_2338_cov_4.575558"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1573)
                     /locus_tag="PKI33_10770"
     CDS             complement(140..1573)
                     /locus_tag="PKI33_10770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM37540.1"
                     /translation="MKFSFPVKIYLLALVLRLIPVFLTMNMGIGLDDMFQYDMLARSL
                     ASGHGFRWYALEDLKRLEPYVSLDLSTASDYDPRYGVYTSFRAPLYPTFLAFIYSLSG
                     LGFSRFFAARCAQAILLGAPLAPLTYGIAKRFFVDIDSNEKISRLAASIVACYPMLLV
                     YPLGLGTENLFFILLLFSFLFLLRTLAAPSKLNFLFAGVFLGLAALTRSVVLPFVGVS
                     LFYLFYLHRTKAWITFVAFCLIISPWMIRNSLLHHKLTGIETSMGYNLYLGYYPEGNG
                     SFIFGPSLDLLPIMDDSERDTIGTQKAIEFIRAEPGRFIPLALNRLGFFFGLENRVLM
                     YFYSNNLLGYISTPILLLISAFFLLPFVFVSILAVFGLVAQKPSQPRALLHLLLFAYI
                     MPYVLILSEDRFHLALIPFLAMLASYTFVLLKERRYVHYVSIRTLALAGILIILLVLN
                     WGLELHRDADKIAAILQPNGNQLYLPY"
     gene            complement(1570..2088)
                     /locus_tag="PKI33_10775"
     CDS             complement(1570..2088)
                     /locus_tag="PKI33_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNM37541.1"
                     /translation="MFTQPKSTASYLNPKCEARPKRVTGCAVFAIAPISKGELISLWG
                     GNIIHKSQLDQTMPRFTERVIQIDEDLYLLTSAEKEPNDCFNHSCEPNLGFTGQIGLI
                     AMRDIQTGEELTFDYAMSDGGPYDEFECDCGNLTCRHKVTGNDWALPELWQKYEGYFS
                     SYLARRIENLRK"
BASE COUNT          769 a          474 c          568 g          527 t
ORIGIN      
        1 ttttatggta atagtccacc atcaacaata aggtgctgac aatggtcact gtaacgacct
       61 tccagtcaaa acttagcttt tcgcctgtca aatatttcat gcgttacctc tgagtataaa
      121 atcgccccag cctgtgagat taatatggca aatacaattg attgccattt ggctgaagga
      181 tggcggcaat tttatccgca tcgcggtgta attcaagccc ccaatttagc accaagagga
      241 ttatcaatat gcctgcaagt gccagtgttc taatagatac atagtgaaca tatcttcttt
      301 ctttcaaaag cacaaaagta tacgatgcca gcattgccaa gaacggaatc aacgccaagt
      361 ggaatcggtc ttcggataag atcaaaacat aaggcatgat ataagcgaac aaaagtaaat
      421 ggagcaaagc tcttggttgt gaaggttttt gtgcaacaag cccaaaaact gcaagaatac
      481 ttacgaatac aaaaggcaaa aggaaaaatg ccgaaatcaa aagcaatatc ggggtggaaa
      541 tataccctaa gagattgttt gagtaaaaat acatcaatac gcggttttcc aagccaaaaa
      601 agaacccaag gcgattcagt gccagtggaa taaaacgacc aggctcagca cggataaatt
      661 caattgcctt ctgtgtgcca attgtgtccc gttcagaatc gtccataata ggaagtagat
      721 caagggatgg tccaaaaata aaggagccat ttccttcagg ataatagcca agataaaggt
      781 tatatcccat ggaggtttcg atcccagtca atttatgatg aagcaaagag ttacgaatca
      841 tccaagggga gatgattaag caaaaagcga caaatgtaat ccacgcttta gtgcgatgga
      901 gataaaacaa ataaaaaaga ctcactccaa caaaaggcaa aaccacagaa cgtgtaaggg
      961 cagctagacc tagaaaaacg cctgcaaaca aaaagttgag tttagatggg gctgcaagag
     1021 ttctgagaag gaacaagaaa gaaaaaagaa gcaggatgaa aaaaagattc tcggtgccta
     1081 acccaagtgg gtaaacaagc agcatggggt aacaggcaac aatcgatgct gcgaggcgag
     1141 aaatcttttc gtttgaatca atatcaacaa agaaacgctt cgcaatacca tatgtgagtg
     1201 gcgcaagagg cgcacctaaa agaatggctt gggcgcaacg ggcggcaaag aagcgcgaaa
     1261 agccaagtcc gctaagggag tagatgaacg ctaaaaaggt cggatatagt ggcgctcgaa
     1321 aagacgtgta taccccatac cgtgggtcgt aatcagatgc agtggaaagg tcgagactca
     1381 catagggttc gaggcgtttg aggtcttcca aggcgtacca gcggaatcca tgcccggagg
     1441 cgaggctacg agccagcata tcgtactgga acatatcatc cagtccgata cccatgttca
     1501 tggtcaggaa gaccggtata agtcgcaaaa cgagcgcgag cagataaatt tttacaggga
     1561 aagaaaactt catttcctca aattttcgat ccggcgggca agataggatg agaaatagcc
     1621 ctcatatttt tgccaaagtt ctggcaatgc ccaatcgttc ccggtcactt tgtgacggca
     1681 agttaggtta ccacaatcgc attcaaattc atcatatggg ccaccatcag acatggcgta
     1741 atcgaaggtc aattcttcgc cagtttgaat atcacgcatg gcaatcaaac cgatctgccc
     1801 cgtgaagcca aggtttggct cacacgaatg attgaagcaa tcgttcggtt ctttttctgc
     1861 ggaagtaagc agataaaggt cttcgtcgat ctgaatgact cgttctgtaa aacgcggcat
     1921 ggtttgatcg agttgacttt tgtgaataat attgcctccc caaagggaga tcaattcgcc
     1981 tttggaaata ggagcaatgg caaaaacggc acaaccggta acgcgcttgg ggcgagcttc
     2041 gcatttcggg tttaagtaag aggcagtcga tttgggttga gtaaacatgg tcaataggaa
     2101 gaatcaggtt cgatacggga aagaagcgga agccagaaag ccgtattgct ttctggcttt
     2161 atccaattat tttgagtcgc cttctgtatc cgcggaggaa gcagagatac gttgggcgaa
     2221 aaagatcggg agaaaggtaa ttagtaccac aatggttgcg acaacatttg tcacggggcg
     2281 gtcacgtggg cggacgagtt ggctgaaaat ccaaataggc aaagtagcct gttgccca
//
LOCUS       DAOREF010000518         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34329_length_2335_cov_4.390789, whole genome shotgun sequence.
ACCESSION   DAOREF010000518 DAOREF010000000
VERSION     DAOREF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34329_length_2335_cov_4.390789"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PKI33_10780"
     CDS             complement(<1..780)
                     /locus_tag="PKI33_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37542.1"
                     /translation="MLISLACGTSGSTASRESTVEAISAQVRATGTAAAAQSSQGTQP
                     VVETNSDSLTATAVAFAPILESLPKYGVDPSEGKIGWIHPPVSLDASGYHQFEYINYY
                     IGTLAKDFVISSDITWNTVGSISGCGFVMRSDGNTNALDQYVAVITRVASGHFVFSTM
                     SDGEVVTGRDIYARYKDKSFRWENDTTNRLTIVGRGNHFWIYTNDTLIGEVDPSAPPP
                     QPNLPPEPDKPANPSDPQAMALYEQQKIEREAIIVQMQADYA"
     gene            complement(826..>2335)
                     /locus_tag="PKI33_10785"
     CDS             complement(826..>2335)
                     /locus_tag="PKI33_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37543.1"
                     /translation="FLARGGYLAWFGPPDEALAYFDQYRSERARRAGKIEFDEIYAIL
                     DDPSKGKAEDWANRYRSSNAHTEYVLKPLASKLSPQSVATIPQPERLPKKATNTRQVS
                     SLRQLMILSARNIKILTRDRFALGLMLATAPVVSLLDVILSFVMGREIFDFYKGDMSQ
                     VMITLFLLTIYGIMVGGISQMREIVKEQDIYRRERLVNLKIFPYVLSKVWVAALLALY
                     QAVAYTVVHYISFRMPGGVMEFIQIYITMTLATFAGMMLGLFASALAPNSSSAPLLVI
                     ILMLPQIVLAGALIPVPSFVSAPFSTRWAFESLIAISGSGSDVAADVCWALPPDVRNA
                     MSLEEKLANNCKCMGVNLLKKESCNHPGIGKFYIPLVDQPLPIPPAPLPPEPVRPIEP
                     QDQSDNIAMADFFAKLKIYEAEVELYKAKVDKYQEEQVAYQTVISQLKVQVATAEAVV
                     RTFVNGSGFAYVDKDDPLAYHTKVILTWSVQSFIIVLLFVAILYLQKRKDVI"
BASE COUNT          645 a          542 c          524 g          624 t
ORIGIN      
        1 agcataatca gcttgcattt gtacgatgat tgcctcgcgc tcaattttct gctgttcgta
       61 caatgccatc gcttgcggat cggatggatt ggcaggctta tccggttcag gtggaagatt
      121 gggctggggt ggtggggcgc ttgggtcaac ttcaccgatc aaagtgtcgt tggtgtaaat
      181 ccaaaaatga tttccacgcc ctacgattgt cagacggttg gttgtgtcat tttcccatct
      241 gaaacttttg tctttatatc ttgcataaat atccctacct gttacaactt cgccatctga
      301 catggtggaa aaaacaaaat gtccacttgc tacacgggta atgactgcca catattgatc
      361 caatgcattt gtattgccgt cagacctcat gacaaacccg cagcctgaaa tactgccgac
      421 agtattccaa gtaatgtcag aagatattac aaagtctttc gccagtgtac caatataata
      481 atttatgtat tcaaattggt gataaccaga tgcgtccagc gatactggcg gatggatcca
      541 gccgatctta ccctctgaag gatcaacgcc atatttgggc aggctctcca gaattggagc
      601 aaatgccact gctgttgctg tcaatgaatc tgaattcgtc tccacaactg gctgggtgcc
      661 ctgtgaactt tgtgctgctg ccgctgtacc agttgcacgg acttgggcac taattgcctc
      721 aacagtactt tctcttgagg ctgtgctgcc gctggttcca caagccaacg agattagcat
      781 aaagatcaac gggaatatta tgagtctatt ttttcgcatc gggctctaaa tcacatcttt
      841 acgtttctgc agatataaaa tagcaacaaa caataagaca ataatgaagc tttgcacgga
      901 ccaggtcaat ataaccttgg tgtgatatgc caacgggtca tctttatcaa catatgcgaa
      961 acctgatcca ttgacaaagg tccggacaac ggcttcggca gttgcaactt gcacctttag
     1021 ctggctgatt actgtctgat aggcgacctg ttcttcttga tacttgtcaa cctttgcttt
     1081 ataaagctca acttcagctt cgtagatttt gagtttggca aagaaatctg ccatggcaat
     1141 attgtctgat tgatcttgtg gctcaatagg gcgaacaggt tccggcggta atggcgcggg
     1201 cgggattggc aatggttggt ccacaagcgg gatgtagaac ttgccgatgc caggatgatt
     1261 acaagattcc tttttaagta gattgacacc catgcatttg caattattgg caagcttttc
     1321 ttccaaagac atcgcattgc gaacatcagg tggtagtgcc caacaaacat ctgccgcaac
     1381 atcagaccca gaccctgaga tggcgatcag agattcaaat gcccaacgtg ttgagaaggg
     1441 ggcgctcaca aaacttggaa ccggaattaa tgcaccagcc aatacgatct gaggcagcat
     1501 taaaattatg acaagtaggg gggcagaact ggagttgggc gccaatgctg aagcaaacaa
     1561 cccaagcatc attccagcaa aggtggcaag agtcatggtg atatatatct ggatgaactc
     1621 catcactccg cccggcattc taaatgaaat ataatggacc accgtatatg ccactgcctg
     1681 atagagtgcc aataaagcag cgacccatac ctttgacaat acatacggaa aaattttgag
     1741 attgaccaac cgctctcgcc gataaatatc ctgttcctta acgatctcgc gcatttgaga
     1801 aattccgccg accataatgc cgtatatcgt taagaggaaa agagtgatca tgacttgaga
     1861 catgtcacct ttatagaaat caaaaatctc ccgccccatt acaaaagaaa ggattacatc
     1921 taataaactt accactggcg cagtggcaag cattaatccc agcgcaaaac ggtcacgggt
     1981 caatattttg atattacgag cagataggat cattaactgt cttaatgaag agacctgtct
     2041 cgtattagtt gcttttttgg gtagtctttc gggctgcggt atcgtcgcca cactttgtgg
     2101 cgacaacttg ctagccaacg gttttaagac atattccgtg tgggcattac tggagcggta
     2161 tcgatttgcc caatcctctg ctttgccttt gctgggatca tccaaaatgg catagatctc
     2221 atcgaactcg atcttgccgg ctcggcgagc ccgctcagaa cgatactggt caaaataagc
     2281 tagcgcttca tcaggaggac caaaccatgc taaataacct cctcttgcga gaaag
//
LOCUS       DAOREF010000519         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34355_length_2334_cov_4.088197, whole genome shotgun sequence.
ACCESSION   DAOREF010000519 DAOREF010000000
VERSION     DAOREF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34355_length_2334_cov_4.088197"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="PKI33_10790"
     CDS             <1..1888
                     /locus_tag="PKI33_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37544.1"
                     /translation="YMVLMFTPWLYARARRSIFNNENLQALMAVLYVNTVFTMWATLG
                     YSGPSWLPTFHRTMAFISLKANSLQSGIGDLIVKLFATIVQVLRFPHRFELVTFMMAC
                     IIISISLVWLHDVFMQTTYPKLVWILTGKHLPAEKKSRRQNQVAPKKEAGRFIPLLMA
                     VLFMFPLFSNQSYRIVFSSGDFNQFLTPYPVGPLKEVKNALLKLPPGKVVVLPPTETA
                     KVVIDINGVEHKFIDKFHIYYLDLPSYYYGLTGDSDNKHEFFLLLRALYYQQPWWINI
                     ARDLDLKYIVVNKELVASTVGGQEYLKEVEKILIPELDSRDAYVKKVMENESYVLYEF
                     TDLPTAERVPLYLDVDWNSFIRILSSNLELTRYYDLRHTMVVGDLEDFDSLTMVTDDE
                     HESALDLYEKKNKKQFFRPSSVILPFDPEQISSSYYLSPMFRLFQFFSDSKYNRLDMI
                     TPGLWGTLEGGFIGVPREAPFRIDVTLPEEGEYRLLMRGAISAVDIEMESKLFPEPQR
                     ISLASDPSNLAFFDKRLVFSSSRTPLDVSKYTYQELGMLIPTDVVVVNYQYQFFDLGV
                     VQASKGKYPIYFNKLNDAPMLVEGILVIPEEVYQTLTLPSNVTVVQPNDLCCGSVIIQ
                     GEEP"
     gene            1996..>2334
                     /locus_tag="PKI33_10795"
     CDS             1996..>2334
                     /locus_tag="PKI33_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37545.1"
                     /translation="MELIIMLKKYFIFLSTHVNAILNRLRTKIPGLPSNRVLLSTAGV
                     VVAAGGIFVALQVLVAPDQPVTTYTLPYTQTFDDVNLKRWFSQSGVWTIRTGTLMQTV
                     GGEEAGQMHIP"
BASE COUNT          578 a          441 c          597 g          718 t
ORIGIN      
        1 ttatatggtg ttgatgttca ccccttggtt gtatgcacgg gcaagaagat ctatatttaa
       61 taatgaaaat ttacaagcct tgatggcggt tctctatgtg aacaccgtgt tcacaatgtg
      121 ggcaacgttg gggtattcgg gaccttcctg gttgccgact tttcatcgta cgatggcgtt
      181 tattagtttg aaggcaaata gcctgcaatc tggcatcggg gatttgatcg tcaagttgtt
      241 tgcaacaatt gtccaggtgt tgcgttttcc gcaccgcttt gagttggtca cgtttatgat
      301 ggcgtgcatc attatctcga tcagcctggt atggctgcat gatgtcttta tgcaaacgac
      361 ttacccaaag ctggtgtgga tattaaccgg aaaacatctt ccggctgaga aaaaatcgag
      421 aaggcagaat caggttgcgc cgaagaaaga ggcggggcgt tttatcccgc tgttgatggc
      481 ggtactgttt atgttcccgc tgttctccaa tcagtcgtat cgtattgtgt ttagctctgg
      541 agatttcaac caatttctga cgccataccc ggttggaccg ttgaaggaag taaagaacgc
      601 tctgttgaag ttgccgccag ggaaagtagt ggtgctaccg ccaacagaaa cggcgaaggt
      661 agtaattgat atcaacgggg tggagcataa gttcattgat aagtttcata tttattacct
      721 tgacttgccc agttattact acggtttgac gggcgactcg gataacaagc atgagttctt
      781 tttactttta cgggctttgt attatcaaca accttggtgg atcaatattg cccgtgattt
      841 ggatttgaaa tacattgtgg ttaataaaga gttggtggca agcactgtgg gcggacagga
      901 atatttgaaa gaagtggaaa agattttgat tcctgaattg gattcacgtg atgcctatgt
      961 taagaaggtg atggaaaacg aaagttatgt gctgtatgaa tttactgacc tgcccacagc
     1021 ggaacgcgtg cctttgtatt tggatgtgga ttggaattca ttcattcgga ttctgtcttc
     1081 taatttggaa ctgactcgtt attatgacct gcgtcatacc atggtggtgg gggatttgga
     1141 agattttgac tcgctgacga tggtgacgga tgatgaacac gaatcagcgc tggacttgta
     1201 tgagaagaaa aataagaagc aatttttccg tccgagtagt gtgattttgc cattcgaccc
     1261 agagcagatt tcaagttcat attatctctc gcccatgttt cgtttgtttc agttcttctc
     1321 ggatagtaaa tacaatcgct tggatatgat cacgccgggc ttgtggggca cgttggaggg
     1381 cggtttcatt ggagtgccgc gtgaagctcc atttcgcatc gatgtgacat tgcctgaaga
     1441 gggtgaatat cgcctgctga tgcgtggtgc gatctcggca gtggatatag aaatggagtc
     1501 gaagcttttc ccggaaccgc agcggatttc gcttgcttca gacccatcaa atttggcatt
     1561 tttcgacaag cgtttagtct tttcttcaag ccgcactccg ttggatgtga gcaaatatac
     1621 ctaccaagaa ttgggaatgc ttatcccaac cgatgtggtg gttgtaaatt accaatatca
     1681 gttctttgac ttgggtgttg tacaggcatc caaagggaaa tacccgatct atttcaataa
     1741 gctcaatgat gcacctatgt tagtggaagg cattttagtg attcctgaag aggtttacca
     1801 gacccttaca ttgccttcca atgttacggt ggtgcaaccc aatgacctgt gctgtggttc
     1861 ggtcattatt caaggggaag agccttagag ttaacgcaaa ataacaatgg gggaaaacct
     1921 gtatttattg atttgcaaaa tctttagttt tttgaaaacg agtctgaaat gtagtatttt
     1981 tctattttca ctagaatgga attaattatt atgctgaaga agtattttat tttcctttct
     2041 actcatgtga atgctatttt gaacagactg cggacgaaga ttcccggttt gccttcgaat
     2101 cgcgtattgc tatccactgc tggagtggtg gtggctgctg gcgggatttt tgtggcattg
     2161 caggtactgg ttgcgcctga ccaacctgtg acgacttaca ccttgccata cacacagacg
     2221 tttgatgatg tgaatctcaa acgctggttt tctcaatctg gtgtgtggac gatccgtacg
     2281 gggacgttga tgcaaacagt tggcggagaa gaagctgggc agatgcatat tccg
//
LOCUS       DAOREF010000520         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34441_length_2331_cov_3.763620, whole genome shotgun sequence.
ACCESSION   DAOREF010000520 DAOREF010000000
VERSION     DAOREF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34441_length_2331_cov_3.763620"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKI33_10800"
     CDS             <1..819
                     /locus_tag="PKI33_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37546.1"
                     /translation="TFEKFERPFNASTMDVYYSEIDIVNTLVYQDATWIYGIITLNQL
                     QGNGLENVKYAIELDTDIDGKGDWLIIASKPSSTEWVVEGVQVFRDANKDVGGEQPML
                     TDNNPVAGDGFETIIFDQGNGSDPDIAWVRISPNDPNTIELAFKQTTLESPKRYLINM
                     WAGTKLIDPALFDISDNFTHEQAGAADTGLNIFYPIKEVAEIDNSCRMAIGFKPTGKE
                     DGLCESFVPGSPEQVLQPGGPGCSATQSDIDACNATAAMSWHAASCSCVSDPPK"
     gene            1104..1496
                     /locus_tag="PKI33_10805"
     CDS             1104..1496
                     /locus_tag="PKI33_10805"
                     /inference="COORDINATES: protein motif:HMM:NF036989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37547.1"
                     /translation="MTETEKITINLGAVDLGKIDLLVEQGHYSNRTDFIRSAIRSQLD
                     KHVLEVQQAVTRYSFGVGAFFHSRADFEKYKAKGDKIKIDVIGIYHIPDDVSIALALE
                     VVESIKVYGIFQARSELKDTLQKAGKIR"
     gene            1493..>2331
                     /locus_tag="PKI33_10810"
     CDS             1493..>2331
                     /locus_tag="PKI33_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37548.1"
                     /translation="MTSEHQLPENWATRFFTIFAGQAFSLFGSALVQFALIWYLTQKT
                     GSATVLATASLFGMLPQVLLGPIAGTIVDRGNRRLIMILSDSLIAISTLLLAYLFWSG
                     AAEIWHIYVALTIRSAGGAFHYPAMASSTSLMVPDKHLSRVAGANQTLQGLISIVAPP
                     VGALLVAAMSTQNILMIDVITAIMGVTPLLFFTIPQPPRKAEQTATQTSFLKDLGAGF
                     KYMASWPGLLMLGLLATVLNFLLAPLTALMPLLVTKYFKLGALELGSLDSFFGIGMIA
                     GGIT"
BASE COUNT          667 a          553 c          483 g          628 t
ORIGIN      
        1 acatttgaga aatttgagcg tccttttaat gctagcacca tggatgtcta ttattctgag
       61 atcgatattg tgaacacctt ggtctatcag gatgccacct ggatttatgg catcatcacc
      121 cttaatcagc ttcaagggaa tgggctggaa aatgttaagt atgcgattga attggataca
      181 gatattgatg gcaaagggga ttggttaatt attgcaagca aaccctcttc cactgaatgg
      241 gttgtagaag gtgtacaggt ctttagagat gccaacaaag atgtcggcgg cgagcaaccc
      301 atgctgactg ataacaatcc agtagcaggc gatggattcg aaaccataat ttttgaccag
      361 ggtaatggaa gcgatccaga catcgcctgg gtacgcatct ctcctaatga tcccaatacg
      421 atcgagctcg ctttcaaaca gactacatta gaatccccga aaaggtatct catcaacatg
      481 tgggctggca caaaactgat cgatcccgct ctcttcgaca tcagcgataa tttcacccat
      541 gaacaggcag gcgctgcaga taccgggctg aacatcttct accctatcaa agaagtggct
      601 gaaatagaca actcttgccg tatggcaatt ggcttcaagc caactgggaa agaagatggt
      661 ctatgtgaat cctttgttcc tggtagccca gaacaagtgc ttcaacctgg tggacctgga
      721 tgttcagcca ctcaatctga tattgatgca tgcaatgcaa ctgcagccat gtcatggcat
      781 gcagcaagct gttcctgtgt atctgatcct cccaaataat tcacgtaaaa ttcaggttaa
      841 agtcccccgt tactatagac aggggacttt ttttatggaa attgatccaa aaattatttc
      901 aatatggata aggtctttcg caaataaaaa actcttacga aattatttat tcatcgtcac
      961 gtgatgcatg ttagtaaaga atgttgccag aaaacgaaat aaacatccat ataacatcca
     1021 tttggatgta aaaaacttga caattccaaa aaaacacaat aaaatacgaa aaataccatc
     1081 cacacagatg gtaaaggaga gaaatgacgg aaacagagaa aattacaata aacttaggtg
     1141 ccgtagattt aggcaagatt gatctgctgg tcgaacaagg acactactcg aaccgcacag
     1201 acttcatccg ctcggcgatt cggtcacaac tagataagca cgtacttgag gtacagcaag
     1261 cagtcacccg ctattcattt ggtgtcggtg cattctttca tagccgtgca gactttgaaa
     1321 aatataaagc caagggcgat aagatcaaaa tcgatgtcat tgggatatac catattccag
     1381 atgacgtttc catagccctt gcgctggaag tcgttgagtc cattaaagtg tatggcatct
     1441 tccaagcgcg tagtgaatta aaagacaccc ttcagaaagc agggaaaatc agatgacaag
     1501 cgaacatcaa cttccagaaa actgggctac ccgtttcttc accatctttg caggtcaggc
     1561 tttttcatta ttcggctctg cattagttca atttgcatta atctggtacc ttacccaaaa
     1621 gacaggttct gcaaccgtac ttgctaccgc atctttattc gggatgcttc cacaggtact
     1681 tttaggaccc attgcaggaa ccattgtgga tcgaggcaac cgtcgcttga tcatgatcct
     1741 atcagactca ctgattgcga tctctacgct tcttctcgcc tacttattct ggtctggcgc
     1801 tgcagaaatt tggcatatct atgtagcctt aaccatccga tctgcaggcg gagctttcca
     1861 ctaccccgct atggcatcat ctacctcgtt aatggttccc gataaacacc tctcgcgtgt
     1921 agccggtgcc aaccaaacct tgcaagggct tattagcatc gttgctccac cagtaggcgc
     1981 tctgcttgtc gcagccatgt ctacacaaaa tattttgatg attgacgtaa ttactgccat
     2041 tatgggtgtc acaccgttgc ttttcttcac cattccacaa ccgccccgta aagctgagca
     2101 aaccgctacg caaaccagct ttcttaaaga cctcggtgca ggtttcaaat atatggcatc
     2161 ctggcctggt ctactcatgt taggtttact ggcaactgta ttgaacttcc tcctcgctcc
     2221 cctcacagcg ctcatgccat tgttagttac gaaatacttc aaattaggtg ctctcgaatt
     2281 aggcagcctc gactcattct tcggcatcgg catgattgcc ggcggaatta c
//
LOCUS       DAOREF010000521         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34601_length_2325_cov_3.259912, whole genome shotgun sequence.
ACCESSION   DAOREF010000521 DAOREF010000000
VERSION     DAOREF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34601_length_2325_cov_3.259912"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKI33_10815"
     CDS             complement(<1..728)
                     /locus_tag="PKI33_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37549.1"
                     /translation="MNNFFSRFFRIGAQLFFALTIILMPFRLRLVLWTRPFFPVYSDY
                     TDFLLFASDLGVLFILGLWASSLIVSPRKVKVGNPFIWYLLLGITLSAWMSILGSVDP
                     IITRYHAVRFVFLLLFYLYIVNEIVSIAWVIIPVAIQAVIQAPIAIAQFVKQSSLGLQ
                     VLGEDTLDPLVLGTSIIPVNGERILRAYGLTDHPNIAGGCLTFVLVLLFSVFFYGKKW
                     QFFVAGTVIVPTFLALLLTFSRSA"
     gene            822..1913
                     /locus_tag="PKI33_10820"
     CDS             822..1913
                     /locus_tag="PKI33_10820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM37550.1"
                     /translation="MRVAIVHEWLSTFGGSERLLVQLLQLFPHADIFALTHFPKNFQH
                     TELKDIRVHTTFLQNLPNIERQYRKLLPIMPLAIESLDLSAYDLVLSLSHAVAHGVKT
                     HKNQAHISYISTPMRYAWHMRDDYLKLHGLTNPLVRLAANGTLSLLRRWDAFASARSD
                     SLIANSQWTASHIHEAWGRDSQVIYPAVDISRFTPSTQRENYYLLVSRLVPYKLGIEI
                     VKAFNSLKLPLIIVGDGPELQHIQEIAKENITVMGYQPDAVVTELMNKAKAFVYMATE
                     DFGIAMVEAQAAGCPVIAYYKGGASEIIRDGETGLLFKEQTAVSLAEAVTTFEAMNLN
                     SKAASDNAARFSSERFRREFLDHVHKFLE"
     gene            complement(1946..>2325)
                     /locus_tag="PKI33_10825"
     CDS             complement(1946..>2325)
                     /locus_tag="PKI33_10825"
                     /inference="COORDINATES: protein motif:HMM:NF021495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HNM37551.1"
                     /translation="FIALRKSISSVEVRRIQQVDLWYGISAGVLLVAGLLRVFIFEKG
                     ASYYGANPLFWVKMALFALVGILSIYPTIRYIRWGNVTEDKNLELPDDEYKRIRLLLN
                     LEVIGIALILFAAPAMARAIGMN"
BASE COUNT          740 a          582 c          463 g          540 t
ORIGIN      
        1 caagcagagc gagaaaaggt taataacaaa gccaaaaagg ttggcacaat cactgtacca
       61 gcaacaaaaa actgccattt tttgccataa aagaaaacag aaaacaagag aacaaggaca
      121 aatgtcaaac atccgcctgc aatatttggg tgatctgtaa gcccataagc acgcaagatc
      181 cgctcgccat tgacggggat aatactcgta cctaacacaa gcgggtcaag ggtatcttca
      241 ccgagcactt gcagcccaag cgaagactgt ttcacaaatt gagcaattgc aataggcgct
      301 tgtatcactg cctgaattgc gacaggaata atcacccaag cgatagaaac aatctcattc
      361 acaatataca aataaaacaa aagcaaaaat acaaagcgaa cggcatgata ccgagtaata
      421 attgggtcaa cgctgcctaa aattgacatc catgccgaaa gggtaattcc caataacaga
      481 taccagataa aaggatttcc aaccttcact ttgcgaggtg aaactatcaa cgacgatgcc
      541 caaaggccca atataaataa aaccccaagg tcactggcaa ataaaagaaa atcggtgtaa
      601 tcagaataca ctggaaaaaa tggacgcgtc cacaacacca gccgcaaccg aaacggcatg
      661 aggatgatcg tcaacgcaaa gaagagctgc gcaccaattc taaaaaagcg ggagaagaaa
      721 ttattcacaa caacaaataa gacctaagca atccaatttt acaattataa tctgtcgccg
      781 gttatgtttc tgataattcc cacacaaata ttgagaatca aatgagagtt gctatcgtcc
      841 atgaatggct gagcaccttc ggtggcagcg agcgactcct tgttcaattg ttgcagcttt
      901 ttccgcacgc cgatattttc gcgctgactc attttcctaa aaactttcaa catacagaac
      961 taaaagacat tcgggtccac acaaccttct tacagaatct gccaaacatc gagcgccagt
     1021 accgcaaact cttgcccatc atgccgcttg ccattgagtc tttggattta agtgcatacg
     1081 accttgtcct ttcgctctct catgccgtag cccacggagt aaaaacgcac aaaaaccaag
     1141 ctcacatctc ttacatctcg actccgatgc gctatgcttg gcacatgcgg gatgactatt
     1201 tgaaactgca cggcttgacc aatcccctcg tccgcctcgc cgcaaacggg accttaagcc
     1261 tgctccgccg ttgggacgcc tttgcctctg ctcgatctga ttccctgatc gccaactctc
     1321 aatggacagc gtcacacatc catgaagcat ggggacgaga ttcgcaagtg atttacccag
     1381 cagtcgatat cagtcgtttt acaccctcca cacaacgcga aaattattac ctactcgtat
     1441 cgcgccttgt gccatataaa ttgggtatag aaattgtcaa agcttttaac tcgttgaagt
     1501 taccgctcat catcgtcggt gatggacctg agcttcagca tattcaagaa atcgcaaaag
     1561 aaaatatcac cgtgatgggg tatcagcccg acgcggttgt cacagaactc atgaacaaag
     1621 ccaaggcatt tgtgtacatg gcaactgaag atttcggcat tgccatggtc gaagcgcagg
     1681 cagcgggttg ccccgttatt gcttattaca aaggtggcgc atcagagatt atccgtgacg
     1741 gagagacggg tcttttattc aaagaacaga ctgccgtatc attagccgag gctgtaacta
     1801 cattcgaggc aatgaactta aatagcaaag ccgcatcaga caatgcggct cgcttttcat
     1861 ctgaacgctt tagacgggaa tttttagatc acgtccacaa gtttttagaa taagcgcttc
     1921 agtctactgt aaagctggaa gggtgtcagt tcatgccgat ggcacgtgcc attgctggcg
     1981 ctgcaaatag aattagtgca ataccgatca cttccaaatt caatagcaag cggattcgtt
     2041 tgtactcatc atcgggcaac tctaaatttt tatcttccgt tacattcccc cagcgaatat
     2101 aacggatggt tgggtaaata gaaagtattc caaccagcgc aaataaagcc atcttcaccc
     2161 agaacaacgg gtttgcgcca tagtaacttg cccctttttc aaaaatgaac actcgcaata
     2221 acccagccac caataacaca cctgctgaga tgccatacca taaatccact tgctgaatac
     2281 ggcgcacttc tacagaactt attgatttac gcaaagcaat aaact
//
LOCUS       DAOREF010000522         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34654_length_2323_cov_4.601852, whole genome shotgun sequence.
ACCESSION   DAOREF010000522 DAOREF010000000
VERSION     DAOREF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34654_length_2323_cov_4.601852"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKI33_10830"
     CDS             complement(<1..683)
                     /locus_tag="PKI33_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNM37552.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLNVSAQALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLTRDRVYDAIQDLRGGQRVTDPQAE
                     TRYRTLEKYSRDLTQLAREGKLDPVIGRDNEIMRLIQILSRRTKNNPVLIGEAGVGKT
                     AIAEGLAQK"
     gene            933..2003
                     /locus_tag="PKI33_10835"
     CDS             933..2003
                     /locus_tag="PKI33_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37553.1"
                     /translation="MKRLFPFILTTIFILQACGGNAPTEESAAAPQTANTEVAATTAD
                     VAPATAEPIVHVDIPTEGTVSRASAHDNEESANSGNKDVIFGDDFEKNRFERPFTAND
                     MTYLPDLDIVDFSITSDDSFFYIRIDLVGLDLASQSLTGFYGVEFDKNGDGRAEIFLV
                     TQPPYKTEFTADNVFLFIDANGDVGSEQAAKPDASNGDGFDSLLFDLNDSIHPEDPDL
                     AWVRFIDDTKPAIEIAYKKWLFKDGNENFMWSVTASDELINPAEYNFHDAIAETEAGS
                     PTKSNPNYPLKGLAAIDNTCRVPFGFEAKGNEPLGCSVNNIPPEPEEEIDFCTQFPSA
                     CERLPEPPPLQQFFDKEDLKGF"
     gene            complement(2045..>2323)
                     /locus_tag="PKI33_10840"
     CDS             complement(2045..>2323)
                     /locus_tag="PKI33_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNM37554.1"
                     /translation="MPEFEAWIVDQKRKTNLNVVGTDSTGDVSLMNHTLARPVAVVLG
                     NEAKGMSVALKNLCDYVVSIPVEGAVNSLNVACAGSIILWDVYKNSHR"
BASE COUNT          601 a          636 c          511 g          575 t
ORIGIN      
        1 atcttctgcg ccaaaccttc tgcaatggct gtcttcccca cacccgcttc accgatcaaa
       61 accggattgt tcttcgtacg gcgagacaga atctgaatca ggcgcatgat ctcattatcg
      121 cgcccaatga ccgggtcaag cttaccttca cgggcaagct gcgtcaggtc acgcgaatat
      181 ttttcaaggg tgcggtaacg cgtctccgct tgcggatcgg tcacccgttg accaccgcgc
      241 agatcctgaa tggcatcata cacacggtca cgggttaatc ctgcagattc caaaatgcga
      301 gccgcagggg tattacgttc agtaagaatc gccaaaaaga tgtgttcggt tgaaatatat
      361 tcatctttga gacggttggc ttcttcgttg gcaaggtcga taatccgctt gacgcgcggg
      421 gtgatgaaaa tctgtcccgc tccgccgccg aagatgttcg ccttggggct ggcgcgcagg
      481 gtggcatcca gccgttcggt gagcgcttga gcgctgacat tcaatttttc aagaatttgg
      541 ggaatcactc caccaggctg ttcaatcagc gccagcagaa tgtgttcggt atcaatttga
      601 ttgtgcccat agcgttggat aatctccgca gcacgctggg cggcttcttg tgctctctct
      661 gtaaatcggt caaatcgcat cataggggta tcctccggac agaccacaga ccatggatcg
      721 cagaccatgg ttttatggtc tatcgcccat tgtctatcgt ccacccaatg ctggcattat
      781 aacaaacgcc gtatcggcga attgtcagta tggcataaga agagtgttag atttcggcga
      841 ccatcactaa agtaataccc tattccttac ttttttgggt gtagcgccag aaaagaataa
      901 actattatct ccaatatatc gttaggagac ccatgaaacg ccttttcccc tttatcctca
      961 ctacaatttt cattttgcaa gcctgtggcg gaaatgcccc cacagaagaa tcagccgccg
     1021 ctccccaaac tgccaataca gaggtcgctg ctaccacagc agacgttgca ccagctactg
     1081 cagaaccgat tgtgcatgtt gacatcccaa ccgaaggtac ggtgagccga gcctcagctc
     1141 atgacaacga agaaagcgcc aactctggaa ataaagatgt gatctttggt gatgacttcg
     1201 agaaaaatcg ttttgaacgc ccctttacag ccaacgatat gacctatctc cccgatctcg
     1261 atatcgtgga tttctccatc accagtgacg actccttctt ttacatccgc atcgatctgg
     1321 tcggacttga tcttgccagc cagtctctga ccgggttcta cggtgtggag tttgacaaga
     1381 acggcgatgg tcgcgccgaa atttttcttg taacccaacc gccttataaa acagaattca
     1441 ctgctgataa tgtctttctc tttattgatg ccaatggaga cgtaggcagc gaacaagccg
     1501 ccaaacccga tgcttccaac ggcgacggtt tcgactccct actctttgac ctgaacgata
     1561 gcatccaccc cgaagacccc gatctggcat gggtccgctt cattgatgac accaaacccg
     1621 ccattgaaat cgcctacaag aaatggctct tcaaagacgg caacgagaat ttcatgtgga
     1681 gcgttaccgc cagcgacgaa ttaatcaatc ctgctgaata caacttccac gatgccatcg
     1741 cagaaacaga agccggctca ccaacaaagt ccaaccccaa ttatcctctc aaaggtctcg
     1801 ccgctataga caatacatgc cgtgtcccat ttggtttcga agcgaaagga aacgaaccct
     1861 tgggatgttc agtaaacaac ataccgccgg agcccgaaga agaaatagac ttctgtacac
     1921 agtttccatc ggcatgcgaa cgtttgccag aaccgccacc ccttcaacag ttttttgata
     1981 aagaagattt gaaaggattt taattagata gataaaaact gcgcggataa agtccgcgca
     2041 gtttttatct atggctattc ttatatacat cccacaaaat gatcgacccc gcacacgcca
     2101 catttagcga attgactgct ccctctactg ggatgctcac aacatagtca catagatttt
     2161 tgagcgccac actcatgccc ttggcttcat tacccaaaac taccgcaaca ggtcttgcaa
     2221 gggtgtggtt cattagagag acatctcccg tcgaatcggt tcccacaaca ttgagattgg
     2281 tcttgcgctt ctggtccact atccacgctt cgaactccgg cat
//
LOCUS       DAOREF010000523         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34757_length_2320_cov_2.282561, whole genome shotgun sequence.
ACCESSION   DAOREF010000523 DAOREF010000000
VERSION     DAOREF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34757_length_2320_cov_2.282561"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PKI33_10845"
     CDS             <1..836
                     /locus_tag="PKI33_10845"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNM37555.1"
                     /translation="TIKQHKPKVIAHLAGQVAMTTSLQNPRMDFEINGMGTLNVLESV
                     RLHSLDTIVLYSSTNKVYGSLEELDYRETDLRYELTDHPDGLDESLQLDGHSPYGCSK
                     LTGDQYVRDYSRMFGLRTVVFRHSSMYGGRQFATYDQGWIGWFCQKALEMANPNSPIF
                     TISGDGKQVRDVLHARDLVRVYLQAAKHIDRSSGQIYNIGGGMKNSLSLLELFKLLEE
                     NTGFPMRFEKLEWRHGDQKVFVANVGKAGRDFNWTPTVSKRDGIREMMDWSREIFHAN
                     Q"
     gene            823..1542
                     /locus_tag="PKI33_10850"
     CDS             823..1542
                     /locus_tag="PKI33_10850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM37556.1"
                     /translation="MQTNEKKTLTIMIPAYGEAENLRVLLPSIKEITKDLAVPSEIIV
                     LDAESPVDDTQAVCVENQVRWVSRTGGSRYGDAIRTGIRSCTGDFVITMDADGSHNPQ
                     FIYKMWEKRLDADVVIASRYIPGGVTENPWLLVAMSRILNITFKFFVGMPVYDISNSF
                     RLYHGDLLRSIEPKYSDFDILEEILAKIIWGNFTPPAVIIEIPFRFEQRKYGKPKRNL
                     LVFTAQFLIALFKLRWLRFQK"
     gene            1633..>2320
                     /locus_tag="PKI33_10855"
     CDS             1633..>2320
                     /locus_tag="PKI33_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37557.1"
                     /translation="MRIPELDKNVIVRRNKLGMGSIVNFARDGIAFLTGFVTSADLSI
                     FHDFEPPPTGGGHQFLRAFWGQVEARGLRVENNLISRTTRACLFNSFNFRESLLKFTK
                     RDSVLYVHRVDGPIDVYRGRDDGVDRGIQAVNRRFAAKTIFQSNYSLEKHLELGMQFH
                     NPVVIMNAADPDIFHSSGRGAFSRDRKTRLIASSWSDNINKGAPVYQWLDENLDWERY
                     EMTFVGRSPVQ"
BASE COUNT          615 a          464 c          573 g          668 t
ORIGIN      
        1 gaacgataaa gcaacacaag cccaaggtga ttgctcacct ggcagggcaa gtggcgatga
       61 caaccagttt acaaaatcct cgtatggatt ttgagatcaa tggtatgggg acattgaacg
      121 ttttggagtc cgtcaggctg cactcattgg ataccatcgt gctctattct tcaacgaata
      181 aagtatatgg ttctcttgaa gaacttgatt atcgtgaaac ggatctgcgc tatgagctta
      241 ccgatcatcc agatggactg gatgagtcgc tccaattgga tggacactcc ccgtatggct
      301 gctctaagtt aaccggagat caatatgtgc gcgattacag cagaatgttc gggctgcgga
      361 ccgttgtttt tcggcattcc tcgatgtatg gcggcagaca atttgcaaca tatgatcaag
      421 gctggatcgg ttggttctgt caaaaagccc tcgaaatggc aaatcctaac agcccgattt
      481 tcacgatttc tggcgatgga aaacaagtcc gcgatgtgct tcatgcccga gatctggtaa
      541 gggtatattt gcaggctgcc aaacatatcg acaggtcttc cgggcaaatt tataatatcg
      601 ggggcgggat gaagaacagc ttgtcactat tggagctatt caaactattg gaagaaaata
      661 ccggtttccc gatgcgattt gaaaaattag aatggcggca tggcgatcaa aaagtatttg
      721 tagcaaatgt agggaaagcc gggcgggatt ttaattggac gccaacagtt tcaaaacggg
      781 atggcattcg agaaatgatg gattggagta gagagatttt tcatgcaaac caatgaaaag
      841 aaaacgttaa cgatcatgat ccccgcttat ggcgaggcgg aaaaccttag agttttgttg
      901 ccttccatca aggaaataac gaaagacctc gctgtgccat ctgaaatcat tgttttagat
      961 gccgagtctc ctgttgacga tacgcaggca gtgtgtgttg aaaatcaagt tcgttgggtc
     1021 tctcgtacag gggggagccg ttatggggat gctattcgta cgggtattcg ttcctgtaca
     1081 ggtgattttg tcattaccat ggatgctgat ggctcgcaca atccacagtt tatttacaaa
     1141 atgtgggaaa aacgcctaga tgctgatgta gtgattgcgt cccgctatat tcctggcgga
     1201 gttacggaaa acccctggct tctggttgcc atgagccgca ttttaaacat tacttttaaa
     1261 ttctttgttg gtatgcctgt ttacgatatt tccaatagtt tcaggctgta tcatggagat
     1321 ttattgcgca gtatcgaacc aaagtactcc gactttgata ttcttgaaga aattcttgcc
     1381 aagattattt ggggaaattt tacgccgcct gccgttatta ttgaaatccc ttttcgattt
     1441 gaacaaagaa agtacggtaa accgaagcgg aaccttcttg tttttactgc ccaatttttg
     1501 atcgctttgt ttaagttgcg ctggcttcgg tttcagaaat aaaacgtaag gaaatatttg
     1561 atcttgatca ttgagttgga ctataaccct gatgtatatt gactatgcca gataacttga
     1621 agtgtacctc tcttgagaat tcctgaattg gataagaatg tgatcgttcg cagaaataaa
     1681 ttgggaatgg gaagcattgt gaattttgcc agagacggaa ttgcctttct tacagggttt
     1741 gtcacctccg ctgacctttc catcttccat gattttgaac cacccccaac aggaggcggg
     1801 catcaattcc tgcgtgcgtt ctgggggcag gtggaagcgc ggggacttcg ggtcgaaaat
     1861 aacctgattt cacgcacgac tcgcgcttgt ctgtttaatt cttttaattt tagggagtct
     1921 cttcttaagt ttacaaagcg ggattctgtt ctttatgttc atcgagttga tggaccgatt
     1981 gatgtttacc gcgggcggga cgatggggtg gatcgtggga ttcaagcagt gaaccgacgg
     2041 tttgctgcca aaaccatttt tcaatctaac tatagtcttg aaaagcattt agaacttggg
     2101 atgcaatttc ataatcctgt tgtgatcatg aatgctgccg accccgatat attccattcc
     2161 agtggaagag gggctttctc gcgtgataga aagacccgcc tgatcgcatc cagctggtct
     2221 gataatatca acaagggcgc acctgtctat caatggttgg atgaaaacct tgattgggaa
     2281 cgatacgaaa tgacattcgt aggacgcagc ccggtgcaat
//
LOCUS       DAOREF010000524         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34886_length_2315_cov_2.986283, whole genome shotgun sequence.
ACCESSION   DAOREF010000524 DAOREF010000000
VERSION     DAOREF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34886_length_2315_cov_2.986283"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..>2315)
                     /gene="mutS"
                     /locus_tag="PKI33_10860"
     CDS             complement(87..>2315)
                     /gene="mutS"
                     /locus_tag="PKI33_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNM37558.1"
                     /translation="TEPGLLPGDANNYLASVVMEASASSSGTVASVAYTDVTTGEFAV
                     TELPLEALRAELTRLHPAEILYPDNQKLPDGIIGHTTHVPAWKFEPGKCNEVLLTQFK
                     ASTLDGFGLKANSLSVRAAGAIIQYLKETQPDALHLLNSLRSYSLNEFMTLDASTRRN
                     LELDETLRGERKGSLLGTLDATITPMGKRMIHQWVSQPLLNVERINKRQNGVEYFVQN
                     GMVRAELREALRPIADLERLINRVIAGHAQPRDLVAMRSTFANLPNILNALKEKGNKK
                     EAQLPTIDLLGEQLSILQNAIDEDPPATLQNTGVIRPGYSTELDGVIDASKHAREWIA
                     NLESVEREKTGIKTLKVGYNKVFGYYIEISRGAADKAPEHYIRKQTLVNAERFITPEM
                     KEYETLVLNAEERIKEIETRLFKEVCGDLSKTANKILATARSIAELDVLSALGEVAAL
                     NGYTKPQVHEGSELEITEGRHPVVEQLLKSDRYIPNDIIFEKGEIVRVITGPNMSGKS
                     TYLRQTALIVLMAQMGSFVPAGHAKIGLVDRIFTRIGAQDEIHAGQSTFMVEMVETAN
                     ILHHATNRSLLILDEIGRGTSTYDGLSIAWGIIEYVHNHPQLRAKTLFATHYHELTQL
                     AELLPGVRNYNVAVSEADGKVVFLHKIIAGGADKSYGIHVAQLAGLPAPVIGRANEIM
                     TELERSAARGVNVNPNAAQQVALFPETNPLLDELKGIDVNSLSPIEALNKLFEWQKRF
                     GK"
BASE COUNT          562 a          532 c          576 g          645 t
ORIGIN      
        1 aatacggaac caagttccaa ataggatggc agcaattaag attaagatgg ttttatcttg
       61 agtgctgaga gagctttttt tcatacctac ttcccaaatc tcttttgcca ctcgaataac
      121 ttatttagcg cctcaatagg tgacagggag ttgacgtcaa ttcccttcaa ctcatccagc
      181 agtggattgg tctctgggaa gagtgcaacc tgttgagctg cattagggtt gacattcacg
      241 ccacgtgcag cggaccgttc gagttctgtc ataatttcat tggcacgacc gatcacaggt
      301 gcgggaagac ctgcaagttg agcaacgtga ataccatacg atttatctgc gccaccggca
      361 atgattttgt gtaggaaaac aactttccca tcagcttcac taaccgcaac gttgtagtta
      421 cgcacacccg gtaataactc tgccagttgt gttaattcat gatagtgggt tgcaaacaaa
      481 gtcttggctc gcagttgcgg atgattatga acgtattcga tgattcccca tgcaatagac
      541 agaccatcat acgtcgaagt gccgcgcccg atttcatcta aaatcagcaa actgcgattt
      601 gtggcatggt gcaaaatatt tgcggtttca accatttcaa ccataaaggt tgattgccca
      661 gcgtgaattt catcttgtgc gccaatgcgg gtgaagatac ggtctactaa accaatcttt
      721 gcgtgccctg ccggaacaaa cgaacccatt tgcgccatca acacaatcag agcggtctga
      781 cgtaaatatg tagacttacc actcatattc ggtcccgtga tcacccgcac aatctcgcct
      841 ttttcaaaga tgatgtcatt agggatgtaa cggtcagact ttaataattg ctcaacaact
      901 gggtgtctgc cctctgtgat ttccaactcg cttccttcat ggacttgggg cttggtatac
      961 ccattcaagg ctgcgacttc gcctagagca gacagtacat ccagctcggc tatagagcgg
     1021 gcggtggcaa gaattttatt tgcagttttt gagagatcgc cgcaaacttc tttgaataaa
     1081 cgagtttcaa tttctttgat gcgttcttcg gcattcaaga ccagcgtttc atattctttc
     1141 atctcaggtg tgatgaaacg ctcggcattg accaatgtct gcttgcggat gtaatgttct
     1201 ggggctttat cggcggcgcc acgcgaaatt tcgatgtaat agccaaagac tttgttgtag
     1261 ccaactttga gggtcttgat acctgttttt tcgcgttcga cggactctag gttggcaatc
     1321 cattcacggg cgtgtttgga ggcgtcaata acaccgtcaa gttcagttga gtagccggga
     1381 cgaatcacac cggtgttttg tagtgtggct ggcgggtctt catcaatggc attttgtaaa
     1441 atggataatt gttcgcccag caaatcaatt gtgggtaatt gcgcttcttt cttgtttccc
     1501 ttttctttta gggcattgag gatatttggt aaattcgcaa acgttgacct cattgccaca
     1561 aggtcgcgcg gctgtgcgtg cccagcaatc acacgattga tgagacgctc taaatcagcg
     1621 atggggcgta atgcctcgcg caattcagca cggaccatac cattctgcac aaaatattcc
     1681 acgccatttt ggcgtttgtt aattcgttct acatttagta aaggctgact gacccactga
     1741 tggatcatgc gcttgcccat gggggtgatg gttgcatcga gcgttccgag cagggaacct
     1801 ttgcgttcgc ctctcagagt ttcatcgagt tcaaggtttc ggcgtgtgga tgcatccaag
     1861 gtcatgaact cgtttaggct ataagagcgg agcgagttca gcagatggag agcgtctggc
     1921 tgcgtttctt tcaggtactg aataatcgcc ccagctgcgc ggaccgagag gctattggct
     1981 ttcagtccaa acccatctaa ggtagaggct ttgaattgag tcagaagtac ttcattgcat
     2041 ttacccggtt cgaacttcca agcagggacg tgcgtggtat gaccgatgat tccatcggga
     2101 agtttttgat tgtcaggata aagaatttca gcggggtgca ggcgtgtcaa ctctgcgcgt
     2161 aaggcttcga gcggaagttc ggtgacggcg aattctccag tggtgacgtc ggtataagca
     2221 acagaggcaa ctgtcccgga ggatgaggcg gaagcttcca tcaccacaga ggctaagtaa
     2281 ttattcgcgt cgccaggcaa gagtccaggt tcagt
//
LOCUS       DAOREF010000525         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34911_length_2314_cov_3.227092, whole genome shotgun sequence.
ACCESSION   DAOREF010000525 DAOREF010000000
VERSION     DAOREF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34911_length_2314_cov_3.227092"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..410
                     /locus_tag="PKI33_10865"
     CDS             177..410
                     /locus_tag="PKI33_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37559.1"
                     /translation="MNERRKAASFLIRVWQEPSQWQPPGEWRVSAHPLEGGGEVLFNS
                     ATELWRYLTHADSQIQSTPQTKKIISEGEENEQ"
     gene            400..>2314
                     /locus_tag="PKI33_10870"
     CDS             400..>2314
                     /locus_tag="PKI33_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37560.1"
                     /translation="MNNKRSLGCVLTGALIILVICGAIAGFATWRLNTRGPEILVEIK
                     RPQTTMLANVNEALPLIVSAEASDIITRLEVYADGALIAAANGEGQNAAMLAQTWTPS
                     TVGRHVLVARAFLDAEHFSDSEVVFVDALDLSALPLQVSVDDLPRGEGVTEISVGDLA
                     TASGTTPEEIVRLNPSLSSTGSIPSGTLVSIPRLAPEPGIGSPVPPPAPGAPGSPAPS
                     TSDPHFEGETHSCNQIAMRWTGSDFETGYTLYRLAPGDSSMTLVATLPVTTISYADNS
                     ISRAGTYRYFLSPIWRGGGSGITSMLAVEIPADCAPVSSTSANLRLSLMSLTAQESFT
                     GVYCYVSVNGSRYERLPAGDGLLRPTSGNLYYDLPLQLPNRGQYTLGPTADGLVRLEG
                     ECWGRRGVESVRIGRFAGNHASSEWDGRDLTSDVAFEPIEVASLDTIPLMTGGSNFIR
                     YRIQSAASHFDLTGLYNGTIQVPLAITDPFRIENPTVPPPTNVRLVNRASGICEDLPM
                     DDPNLGTILCQEQIVRHIAWDWSASEQVPQSAVTGFMLITSIEDAISTTPAGEGYTHN
                     VNSGAARSAPVPNYSQRWRCGATVRFTVRAVTNFGMSEPSEAFEVRLPACPSARNLLI
                     RIESLTISPSASTG"
BASE COUNT          526 a          593 c          598 g          597 t
ORIGIN      
        1 tcggtgaagc agccgtcctt aaagattttg gtgatggaca tatcacactc gaagcagtca
       61 tcaacggttg gtatgtggta ttggatgcac gcggcttcga cgaaaaaaat ctggcactgc
      121 tggcggaatt attgcttgac aatctggctc cctattcgcc ttaacggagc ttatccatga
      181 atgaaagacg aaaagcagca tcttttctga ttcgggtttg gcaggaaccg agtcagtggc
      241 agccccccgg cgagtggcga gtaagcgctc acccgttgga aggaggcggt gaggtcttgt
      301 tcaactcggc aactgagcta tggcgctacc tcacccatgc agactcgcaa atccagagta
      361 cccctcaaac aaagaagatt atttcagaag gagaagagaa tgaacaataa acgcagtctt
      421 ggttgtgttc taacgggagc gttgataatt cttgtaattt gcggcgccat agcaggcttt
      481 gctacgtggc gattgaatac acgcggacct gaaatcttgg ttgagatcaa aagaccacaa
      541 acgaccatgc ttgctaatgt caatgaagcg ttgccgttga tcgtctctgc tgaagcaagc
      601 gatattatca cccgtcttga agtgtatgca gatggtgctt tgattgctgc cgctaatggt
      661 gaggggcaaa atgctgccat gttggcacag acttggacgc cctctacagt cgggagacat
      721 gtgcttgtag cgcgagcatt tttagatgcc gaacattttt cggattcaga agttgttttt
      781 gtggatgcgc ttgacttgag tgcattgcca cttcaagtga gtgtagatga cctgccgcgc
      841 ggtgaaggtg tgactgaaat ttcagtgggt gacttagcca ccgcctcggg aactacgccg
      901 gaagaaattg ttcggttgaa cccaagtctt tcctctactg gaagtattcc ttcaggcacg
      961 cttgtttcta ttccgcgcct tgctcccgaa cctggtattg gtagccctgt gccgccgcct
     1021 gccccaggtg ctccgggatc ccctgcacct tcaaccagcg acccgcactt tgaaggtgag
     1081 acgcattcct gtaatcagat cgcgatgcgc tggactggca gtgactttga aactggctat
     1141 accctctata gacttgcccc aggcgatagc tccatgacgc ttgttgcgac tttgccagta
     1201 acaaccattt cttatgcaga taactcgatt tcccgagccg gaacatatcg ctatttcctt
     1261 tctcccattt ggcgtggcgg tggcagtggt atcacatcca tgctggcagt ggaaattcct
     1321 gccgattgtg caccagtttc cagcacatca gcaaacttgc gtctttcact tatgagtctc
     1381 actgcgcagg aaagttttac cggcgtgtat tgttatgtct cggtcaatgg gtcacgttat
     1441 gaacgccttc cagcagggga tggcttgttg cgccccacct ctggtaattt atattacgac
     1501 cttcctctgc aattgcccaa ccgcggacaa tatacccttg gtcccactgc cgacggcttg
     1561 gttcgtttag agggagaatg ttggggcagg cgcggagtag aaagtgtgcg cattgggaga
     1621 tttgcaggca atcacgccag tagtgaatgg gatggacgcg acttaactag tgatgtggca
     1681 tttgaaccca ttgaagttgc ttcgctagac accatacctc ttatgacggg tggttccaac
     1741 tttatccgct atcgcattca atctgcagcc agccattttg acttaacggg tttatacaat
     1801 ggcacaattc aggttccgct tgcgattaca gatccatttc gaatagaaaa ccctaccgtt
     1861 ccgccgccca cgaacgtacg tttggtcaac cgtgcatctg gcatttgtga agacctgcca
     1921 atggacgacc ccaatctcgg cacgatcttg tgtcaggagc agatcgtccg tcacattgca
     1981 tgggattgga gcgctagcga acaagtgccg caatcagcag tgacgggttt catgctcatc
     2041 acttctatcg aggatgcaat ctcaactacc ccggctggtg agggctacac ccataatgtc
     2101 aactcggggg ctgcgcgttc tgcacccgtt ccaaactatt cacaacgttg gcgttgcggc
     2161 gcgaccgtgc gattcaccgt ccgtgctgtt acaaatttcg gcatgtcaga accgagcgaa
     2221 gcctttgagg tgcggctgcc ggcttgccca tcagctcgca atcttttgat ccggatcgag
     2281 tcactcacca tcagccctag tgcatctaca gggc
//
LOCUS       DAOREF010000526         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_34981_length_2311_cov_5.115691, whole genome shotgun sequence.
ACCESSION   DAOREF010000526 DAOREF010000000
VERSION     DAOREF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34981_length_2311_cov_5.115691"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /gene="rpsK"
                     /locus_tag="PKI33_10875"
     CDS             <1..304
                     /gene="rpsK"
                     /locus_tag="PKI33_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNM37561.1"
                     /translation="TIVTVTDTEGNTISWGSAGSAGFKGSRKSTPFAARLAAEQAIKS
                     AQQQGLQEVELYVKGPGPGRENAIRAVQAMGLKVLLIADITPVPHNGCRPPKRRRV"
     gene            321..977
                     /gene="rpsD"
                     /locus_tag="PKI33_10880"
     CDS             321..977
                     /gene="rpsD"
                     /locus_tag="PKI33_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNM37562.1"
                     /translation="MAKSLSPVCKLCRREGEKLYLKGERCFTPKCAFDRRSFAPGQHG
                     KTSGRGGNPGSTGRASDFARQLRAKQKARRVYGVLERQFRRYYDTAITRRGLTGLNLL
                     QILESRLDNVVFRLGFASSRAQARLLVTHGHFTVNGRRTDVPSMLLSNGDVIAVREGS
                     GKLSYFKDLNAYAEKRNTPAWLSRDLKAMSGSVARMPERAEIDGSLDEQLIVEYYSRR
                     "
     gene            1049..2017
                     /locus_tag="PKI33_10885"
     CDS             1049..2017
                     /locus_tag="PKI33_10885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNM37563.1"
                     /translation="MTGLITNMVMPKIEREAEARNYGKFVISPLEGGYGVTLGNALRR
                     VLLSSLEGTAITSVRFADVLHEFSDIPGVREDVIQVMLQIKQIRIKMDGVDSARMHLE
                     VRGEGTVTAADIITPAEIEIVNPDTYLFTVDNPKTKLDVEFVVERGRGYSPSNERSGH
                     MPIGELPVDAIFSPVKRVNWEVASARVGQSTNYDKLILEIWTDGTVSPERALSSSARI
                     LIDHLRYIAGVNEEMLTVAVERESSGSRLSSELAETPVEALDLSVRVFNSLKRTGITT
                     VGDVLELLEKGESAVMSIRNFGEKSLDELRDKMREKGFLKDQSSEN"
     gene            2021..>2311
                     /gene="rplQ"
                     /locus_tag="PKI33_10890"
     CDS             2021..>2311
                     /gene="rplQ"
                     /locus_tag="PKI33_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNM37564.1"
                     /translation="MRHSVAGYKLGRTKSSRIALRRNLIKQLFTHERIQTTQAKAAAI
                     RGDAERLITLAKNSADATADQKVHARRVAVSKLGDNQIIKRLFDEIAPRFASR"
BASE COUNT          518 a          660 c          614 g          519 t
ORIGIN      
        1 cacgatcgtt accgttaccg acactgaagg caacaccatt tcctggggat cggctggttc
       61 cgctggcttc aaaggctccc gcaaaagcac tccttttgcc gcacgcctcg ccgctgaaca
      121 ggcgatcaaa tctgctcaac agcagggttt gcaggaagtg gaattgtatg ttaaaggtcc
      181 cggacccgga cgcgaaaatg caattcgcgc cgtccaggcg atgggcttga aagtgttgtt
      241 gatcgcggat attaccccgg tgccacacaa tggttgccgt ccgccgaaac gtcgacgcgt
      301 gtaagtgagg ttgtttattt atggctaaat cattaagtcc ggtttgtaaa ctttgccgcc
      361 gtgagggaga aaaactctac ctcaagggcg agcgctgttt tactccaaaa tgcgctttcg
      421 atcgacgcag ctttgcgcct ggtcagcatg gcaagacctc tggtcgcggc ggcaaccccg
      481 gttccaccgg tcgcgcctcc gactttgccc gccagttgcg cgctaagcaa aaggctcgcc
      541 gcgtgtatgg cgtgcttgaa cgccagttcc gccgctatta cgacacagcg attactcgcc
      601 gtggtttgac cggtttgaac ttgcttcaga ttctcgaatc ccgcctcgat aatgtggtct
      661 tccgccttgg cttcgcttcc agccgcgccc aggctcgctt gctcgtgacc catggtcact
      721 tcactgtgaa cggtcgccgc accgatgtgc cctcgatgct tctctctaac ggagatgtga
      781 tcgcagtgcg cgaaggttct ggaaagctgt cctacttcaa ggacctgaat gcctatgctg
      841 agaagcgcaa taccccagct tggctcagcc gtgatctgaa agcgatgagc ggttctgtgg
      901 ctcgcatgcc cgaacgcgct gaaattgacg gaagtctcga cgaacagttg atcgtcgaat
      961 actactcccg acgctaatcc ttacgcttcg ggtctggacc ttgaactggt tcacgacccg
     1021 aggcttgaac aaaagggaaa ggtgaaccgt gacgggtctc attacgaaca tggtcatgcc
     1081 caagatcgag cgcgaagcag aagctcgaaa ctacggcaaa tttgttatca gtccgctgga
     1141 aggcggctac ggtgtaacgc ttggaaacgc cctgcgtcgc gttttgcttt cctctttgga
     1201 aggcaccgcg atcacttcag tgcgtttcgc ggatgtgttg catgaattca gcgacatccc
     1261 cggcgtgcgc gaagatgtca ttcaggtaat gttgcagatc aaacagatcc gcatcaagat
     1321 ggatggcgta gacagcgctc gcatgcacct cgaagtacgt ggcgaaggaa cggtcactgc
     1381 ggcagacatt attactcctg ccgagatcga gatcgtcaac cccgacacat atctcttcac
     1441 cgtcgataac cccaagacca aactcgacgt tgaattcgtc gtcgaacgcg gacgtggcta
     1501 ttcgccctcc aacgaacgct ccgggcatat gcccatcggt gaacttccgg tggatgccat
     1561 tttcagcccg gtcaagcgcg tcaattggga agtggcttcc gcacgcgttg ggcagagcac
     1621 gaattacgac aaacttattt tagaaatctg gacagacggc acggtttcac ccgaacgcgc
     1681 cctcagctcc tccgctcgca ttttgatcga tcacctgcgt tacatcgccg gcgtgaacga
     1741 agaaatgttg accgtggctg ttgaacgcga atcttccggc agccgcctga gcagtgaatt
     1801 ggcggaaacg ccggtcgaag ccctcgacct ttcggttcgc gtcttcaact ctttgaagcg
     1861 taccggtatc accaccgtgg gtgacgtgct cgaactcctc gaaaagggtg agtccgctgt
     1921 catgtccatc cgcaacttcg gtgagaagag cctggacgaa cttcgcgata agatgcgcga
     1981 aaaaggcttc ttgaaagatc aatcttcgga gaattaagaa atgcgacatt cagtagcagg
     2041 ttacaaattg ggacgcacca agagctcgcg tattgctctg cgtcgcaacc tgatcaaaca
     2101 gctctttacg cacgagcgca ttcagaccac ccaggcgaag gctgctgcca ttcgcggtga
     2161 tgctgaacgc ctcattacac tcgccaagaa cagtgccgat gccaccgcag accagaaggt
     2221 acacgctcgc cgtgttgccg tttccaaact cggtgacaat cagatcatca agcgcttgtt
     2281 tgacgagatt gcccctcgct ttgcttcacg c
//
LOCUS       DAOREF010000527         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35231_length_2303_cov_2.789146, whole genome shotgun sequence.
ACCESSION   DAOREF010000527 DAOREF010000000
VERSION     DAOREF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35231_length_2303_cov_2.789146"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1374
                     /locus_tag="PKI33_10895"
     CDS             163..1374
                     /locus_tag="PKI33_10895"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNM37565.1"
                     /translation="MLETLPVLHQIPTKTWMPRLSFAPKNTAPRGDTLVVVFLRGAAD
                     VLNMVVPHGEEAYYQLRPTLGIARPDDSKKKKEERVVDLDGFFGLHPNMSPLLDAWQT
                     QQLAIIHACGAPDESRSHFKAMELMERGVDDERGPASGWIGRHLATLNTANSSPLRAV
                     GMGTRPQRSLSGSVPVSALRSIADFHLGGDQRALQQMRAALNYVYQEDALGQDTLSIM
                     DTLQKLDPLKYNSPIAYPDSEFGLALKQTAMLIKSEVGLEVSAIDLGGWDTHFTQGSM
                     TGLMPNLMKDLAEGLAAFHADMADHMNKLTTVTMSEFGRRAYENGSLGTDHGHGSMMM
                     VLGGNVDGGKVHGQWPGLEEGQLIGPGDLAVTTDYRDVLSEVLSKRLNNPATSDVFPE
                     YQPVMRNILLT"
     gene            1437..1976
                     /locus_tag="PKI33_10900"
     CDS             1437..1976
                     /locus_tag="PKI33_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNM37566.1"
                     /translation="MKRFAYSLSLLLGFTLACAALTPSATGTIPSTSIAQPQSAQVQS
                     QNAALVDVYDPARDPSADLQQAIDLAQRENKRIMLEVGGAWCVWCKYIDDFFATHADI
                     LQYRDEHYIFMKVNFSEENMNEEFLSQFPEPAGYPHIYVLDSNGKLIHSQNTAELEDG
                     GSSYVPEVFMAFLQKWAEN"
     gene            2014..>2303
                     /locus_tag="PKI33_10905"
     CDS             2014..>2303
                     /locus_tag="PKI33_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37567.1"
                     /translation="MKKYILLFALLLSACNLPGSTASTAPVTEETISEVPQTEDTTQA
                     DSNPPIDIQPAAPLPAGPVHMVALGDSLTQGDGDDSGHGYPGRLLEMVNAVRP"
BASE COUNT          583 a          654 c          539 g          527 t
ORIGIN      
        1 agtctaatcg cgtcgcctgc atttcagtgg cgttagtcca tcgtcattgc gaggagggtg
       61 ctctccccga cgaagcaatc tcgcgcatca tgttatgaga ttgcttaggg caaagagcaa
      121 gagcgccctc gcaatgacgg agaaagttta ttggagataa caatgttaga aaccttaccc
      181 gtacttcatc aaatcccaac caagacctgg atgccgcgcc tctccttcgc gcccaagaac
      241 actgccccac gcggagatac tctcgtagtc gtcttcctac gcggcgccgc ggacgtgctc
      301 aacatggtcg tgccgcatgg cgaagaagcc tattatcaat tgcgcccgac cttgggcatt
      361 gccagacccg atgactcaaa aaagaaaaaa gaagaacgag ttgtagattt ggatggcttc
      421 tttggtttgc atcccaatat gagcccactg ctcgatgcct ggcagactca acaactcgcc
      481 atcatccacg cctgtggcgc gcccgacgaa tcgcgcagtc acttcaaagc gatggaactc
      541 atggagcgcg gcgtggacga tgaacgcggt cccgcctcgg gttggatcgg gcgccacctc
      601 gccacattga acacggccaa ctcatcgcca ttgcgagccg tgggcatggg aacccgtcca
      661 caacgtagcc tgagcggaag tgtccccgtc tccgccttgc gctccatcgc agactttcac
      721 ctcggcggcg atcaacgcgc gttgcagcaa atgcgtgctg ccttgaatta tgtgtatcaa
      781 gaagatgctt tgggtcaaga taccttgtcc attatggata cgctgcaaaa acttgacccg
      841 ctcaaataca actcgccgat cgcttatcct gattctgaat ttggtcttgc cctcaaacaa
      901 accgccatgc tcattaaatc cgaagtcggg ctcgaagtct ctgccattga cctcggcggt
      961 tgggacacgc atttcacgca aggctcaatg actgggctca tgccaaatct gatgaaagac
     1021 ctcgccgaag gtctcgccgc cttccatgcc gatatggctg accacatgaa caaactcacc
     1081 accgtcacca tgtctgagtt tggacggcgc gcttacgaga atggcagcct tggcaccgat
     1141 cacggtcacg gcagtatgat gatggtcttg ggtggcaacg tggacggtgg caaagtccat
     1201 gggcaatggc ccggcttgga agaaggtcaa cttatcggtc ccggcgatct cgccgtcact
     1261 accgattatc gtgatgtgct ttctgaagtg ttgagcaagc gcttgaataa tcctgccacc
     1321 agcgacgtct tccctgagta tcaacccgtg atgaggaata ttctcctgac ctaattgcct
     1381 gacctgacaa aatgcgataa catccaaagg caatccgttc gaaacaggag aatacaatga
     1441 aacgttttgc ctactcactt tcgctacttc ttggctttac cttggcatgt gctgctctca
     1501 ctccttcagc cacaggaaca atcccatcaa catctattgc ccaaccacaa tccgcacaag
     1561 tgcagtcgca aaacgctgca cttgtagatg tttatgaccc agctcgtgac ccttctgcag
     1621 atctacaaca agctattgac ctggctcaac gcgaaaacaa acggatcatg cttgaagtgg
     1681 gcggggcttg gtgcgtttgg tgcaagtaca ttgacgattt cttcgcgacc catgcagata
     1741 ttttgcaata tcgcgatgag cactatatct tcatgaaagt caatttcagc gaagagaata
     1801 tgaacgaaga gttcctctcc caatttccag aaccggctgg ctatcctcat atctatgttt
     1861 tagatagcaa tgggaaattg atccattcac aaaacacagc cgaactcgaa gatggcggct
     1921 catcgtatgt ccctgaagtg tttatggcgt tcctacagaa atgggccgaa aattaaacac
     1981 ctacagtcaa accatacaaa ctgagaaaac accatgaaaa aatacatctt gcttttcgca
     2041 ttactcttat ccgcctgcaa tcttccgggg tctactgctt ccacagcacc agtcactgaa
     2101 gaaaccataa gcgaagttcc tcaaacagag gatacgactc aagcagacag taatccaccc
     2161 attgacattc aacctgcagc cccgctccct gccggacctg ttcacatggt tgccctcggc
     2221 gatagcctta cacaaggtga cggcgacgac tccgggcacg gatatcccgg tcgcctgtta
     2281 gagatggtca atgctgttcg ccc
//
LOCUS       DAOREF010000528         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35292_length_2301_cov_2.995102, whole genome shotgun sequence.
ACCESSION   DAOREF010000528 DAOREF010000000
VERSION     DAOREF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35292_length_2301_cov_2.995102"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKI33_10910"
     CDS             <1..494
                     /locus_tag="PKI33_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM37568.1"
                     /translation="AKEELSNLKAAAMSTFQKVRGFIFELRPMMLDDLGLVPTLRKYV
                     DAFKEQSGMDININVSGTERRLEPYLEVMIFRAVQELLGNAARHSQATLAKIQLDLGA
                     DFIRVAVDDNGRGFDPDILNDSSNLGLKLIRERSEMLGGKFEIDSSIGSGARISFTVQ
                     ARV"
     gene            643..1755
                     /locus_tag="PKI33_10915"
     CDS             643..1755
                     /locus_tag="PKI33_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37569.1"
                     /translation="MRRGRTLILVLLIIIIAIALGAFALRSLVLQPQETQANQPVYVE
                     VYYAAQNIPQGGRITEDVLATLSIPQENVVTVMYTRDELPFLVDKLAKLPLDQGVVIT
                     ESMVTDSSSAVAISGPQWASLIPPGMTAMSIPIDRLSVSGYAINNGAHVNVNACLLFV
                     DVDPTYQSILPNLTAVLTGTGLGGSLTGSGDQGFTTAGLPILSLGVVSSGSAQGRLEL
                     DPSVQQPYYVVPSEAQRPRMVCQTLLQDVVVMRLGDFSLVETDPSATPTQAPDAAPAE
                     QPAPTTPDIVTLIVSPQDSITLTYLVYTNAKISLTLRNPSDQARLATEASTLQFLLSQ
                     YNIPVPAKLPYAMQPAITTLSSPVLSNDAAAQQPQQ"
     gene            1777..>2301
                     /locus_tag="PKI33_10920"
     CDS             1777..>2301
                     /locus_tag="PKI33_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37570.1"
                     /translation="MADKIRVLIVDDIAETRENVRKLLQFEADIEVVGTARTGKEGIQ
                     IAQELNPDVVLMDINMPDIDGITATEQIRQYSTHIQVVILSVQGDQNYMRRAMLAGAR
                     DFLTKPPMGDELISAVKRAGEMAHSEREKGAKHQLSLSASNGVLSIGGFPTATQGKII
                     LVYSQNQKYFLEKKN"
BASE COUNT          635 a          468 c          498 g          700 t
ORIGIN      
        1 aagcgaaaga ggaattgtct aacttgaaag ctgcagctat gagtacattt caaaaagttc
       61 gcggctttat ctttgaactt cgtcctatga tgcttgatga tttagggctt gttcctacgc
      121 ttcgaaaata tgtggatgcg ttcaaagagc aatccggtat ggatatcaac ataaatgtct
      181 ctggtacaga acgtaggttg gaaccctatc tcgaagtaat gattttccgt gctgttcaag
      241 agttattagg aaatgctgct cgacacagcc aggcgactct cgcaaagatt cagcttgacc
      301 ttggcgctga ttttattcgt gttgctgtag atgataatgg acgcggcttt gaccctgaca
      361 ttcttaatga ctctagcaac ctaggtttaa aattaattcg tgaacgatcc gaaatgctcg
      421 gcggaaagtt cgaaattgat agcagtattg gctctggtgc caggatttca tttacagtcc
      481 aagctcgcgt ttaagaataa gtaaatttac aatgttgtta ggaatgtgtg tagatatctt
      541 atctatacat attcctaaca atatgttttt gccatttttc ttgtataatg aatatgttat
      601 aagggcactg gctggaaggt gccattcgag aggagatata ttatgcgtcg cggtcgtact
      661 ctgattttgg ttctattaat cataatcata gcaatagcgt taggtgcttt tgccctgcgc
      721 tcacttgtgt tgcaaccgca ggaaacacaa gcaaatcagc cagtgtatgt cgaggtttat
      781 tatgcagcac aaaatattcc acaagggggc aggataacag aagatgtact tgctactctc
      841 agtatccccc aggaaaatgt tgtaaccgtg atgtacacaa gggatgaact tccgtttctt
      901 gttgataaat tagctaagct tccattggat caaggtgttg taattaccga atctatggtg
      961 acagattcgt catccgctgt ggctatttct ggcccgcaat gggcatcttt gattccgcct
     1021 ggaatgacag ccatgtctat tccgatagac cgcctttctg tttctggcta tgccattaat
     1081 aatggggcac atgtcaatgt aaatgcttgt ttactttttg tagatgtaga tcctacatat
     1141 caatccatac ttccaaactt aacggcagtc ttaacgggta caggcttggg aggctcactt
     1201 actggttctg gtgatcaagg ctttacaacc gctggtttgc caattctttc tcttggtgta
     1261 gtctcttctg ggtcagcaca agggcgcctt gaattagacc cgtcagttca gcaaccatat
     1321 tatgtggttc catctgaggc gcaacgtcct cgtatggttt gccaaacttt attgcaggat
     1381 gtggtggtaa tgaggttggg cgacttttca cttgttgaaa cggatccatc tgcaactcct
     1441 acacaagcgc cagatgctgc tccagcagag caaccagctc ctaccactcc agatattgtt
     1501 acgttaatag tgtcaccaca agattctata acattgactt atttggttta tacaaatgcc
     1561 aagatctccc taacattacg aaacccatcg gatcaagcgc gtttagctac tgaggcttca
     1621 acattgcagt ttttgcttag ccaatataac attccggttc cagcaaaatt accttatgca
     1681 atgcagcctg ctattacaac attgtcctca cctgttttat caaatgatgc tgcggctcaa
     1741 caaccgcaac agtaaccctt attggatttc ttgaatatgg ctgataaaat acgtgtcctt
     1801 atagttgacg atattgccga aacccgtgaa aatgttcgga agctacttca atttgaagct
     1861 gatattgaag tagttggtac agctaggact ggcaaagaag gtatccagat tgctcaagaa
     1921 ttgaatcctg atgtagttct gatggatatt aacatgccag atattgacgg gattactgca
     1981 actgaacaga tccgtcaata ttcaactcat attcaagttg ttattttatc tgtgcaaggc
     2041 gatcagaact atatgcgccg tgccatgttg gctggggcga gagatttctt aacaaagcct
     2101 cctatgggag atgaacttat ttctgccgtc aagcgagcag gggagatggc acattcagaa
     2161 cgtgaaaaag gtgctaagca tcagttgtca ctgtctgcat ccaatggcgt cctatctatt
     2221 ggaggttttc ctactgctac acaaggaaaa ataattttag tttatagcca aaatcaaaaa
     2281 tattttttgg aaaaaaaaaa t
//
LOCUS       DAOREF010000529         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35376_length_2298_cov_3.704860, whole genome shotgun sequence.
ACCESSION   DAOREF010000529 DAOREF010000000
VERSION     DAOREF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35376_length_2298_cov_3.704860"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..>2298
                     /locus_tag="PKI33_10925"
     CDS             146..>2298
                     /locus_tag="PKI33_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM37571.1"
                     /translation="MKIKKAFNALSLLVILGMLLAACAPAATDAPVEAPVATDEAMAT
                     DEPAPAATEAAPATTRHGGWLDEIDFSVVSSDSAVSQLQAGAIDFYSFNLSSNTLQDI
                     KDAGLNYTQSYGGNYGISLNPAVFDDTTVLNPFSNRKIREAVNWLIDRNYINQEIYGG
                     GSLPKLLPITTQLVEYTNLIDTARALETKYAYNLERAREQVAAEMEAMGATMSSDGKW
                     QFNGEPVTLIFLIRSDGDGTRKPMGDYVANQLEEVGFTVDRQYKTSSEAFPIWLGSVA
                     SEGQWHLYTAGYLPSGLTRDERANIQQYYLPTSIQASDPFISNVADPELQKLGDDLAQ
                     GNFTSKEERDAMLARALDLALQDSLFVWVVDQLVYAPYNSNVSVTYDLAAGYEAAFMG
                     NYNLRFVDQEGGTMKVGTNDLFTQPWNTMAGSNWVWDSNIMRATSMGVASSGSASSGL
                     MGDPYTGLAWPQRIESASLEYQTGLPITDNELGWVDVQAVDEIPVPEDAWVDWDATTQ
                     TFITVGQKYPDGTTAKVKSTVVYPADLFETVKWHDGSPISVADFVMSFVIFLDRGKPE
                     SAVYDEAAALSVDAFLPSFKGWRITSTAPLTIEYYSDAYSADAELNVAPLWPGSPSGL
                     SGENSWQILAISNLAEAAGELAYSSDKADANSIEQMSWVGGPSLEILKKYLDQAQTES
                     YVPYEATLGQFLTPEEIALRYENFSKWYADHGHFWV"
BASE COUNT          551 a          566 c          567 g          614 t
ORIGIN      
        1 gaatcaaatt ccctcagaag gcggagtatc agtgagcaga ctggttttcc gccttctgct
       61 tagacgaatt cattaggagg tgctagctga cctatcttgt acgctgttca atttgttttc
      121 caatttcatt aaggagaaaa agagaatgaa aataaaaaaa gcgtttaatg cactcagctt
      181 attggtcatc cttggcatgt tgctggctgc ttgtgcgcct gccgctaccg atgccccagt
      241 ggaagcaccc gttgcaactg atgaagcaat ggcaacggat gaacctgcac ctgctgctac
      301 agaagcggca cctgctacga cccgtcacgg cggctggttg gacgaaattg atttttcagt
      361 cgtttcatct gattcggcag tttctcaatt gcaagccggt gcaattgact tctattcgtt
      421 taacctgtct tcaaatacct tacaggatat taaagatgcc ggcttgaact acacccaatc
      481 gtatggtggt aactatggca tcagtttgaa ccctgctgtg ttcgatgaca caacggtctt
      541 gaatcccttc tcaaaccgta agatccgtga agcggtgaac tggttgatcg accgtaacta
      601 tatcaatcag gaaatctatg gcggcggttc cttaccaaaa cttctgccta tcacaaccca
      661 gttggttgaa tacaccaatt tgatcgatac cgctcgcgct cttgaaacca agtatgccta
      721 caaccttgag agagctcgcg aacaggttgc cgctgaaatg gaagccatgg gtgccacaat
      781 gagctctgat ggcaaatggc agtttaatgg ggaacctgtc accttgatct tccttatccg
      841 ctccgatggc gatggcaccc gcaaacccat gggcgattat gttgccaacc agttggaaga
      901 agtgggcttt actgtagacc gtcaatataa gacatcttct gaagccttcc cgatctggct
      961 tggttctgta gcttctgaag gacagtggca tctttatact gctggttatt tgccctctgg
     1021 tcttacccgc gatgagcgcg cgaatattca gcaatactat cttcccacca gtattcaggc
     1081 gagcgacccg ttcatctcca atgtagcgga ccccgaactt cagaaacttg gtgatgatct
     1141 tgctcaaggt aacttcacta gtaaagaaga acgtgatgcc atgttggcgc gcgccttaga
     1201 ccttgccctt caagactcac tctttgtatg ggttgtagat caactggtct atgctcctta
     1261 caacagcaat gtctcggtaa cctatgacct ggcagctggt tatgaagcgg cattcatggg
     1321 gaattacaac cttcgttttg tggaccaaga aggcggcaca atgaaggtcg gtacgaatga
     1381 cctcttcact cagccttgga acacaatggc gggcagtaac tgggtgtggg actcgaacat
     1441 catgcgtgct accagcatgg gtgtggcaag ctctggcagc gcctcttctg gcttgatggg
     1501 tgacccatac accgggcttg cctggccgca acgtattgaa agcgcctctc ttgaatatca
     1561 aactggtttg cccattactg ataacgaact tggctgggta gatgttcaag ctgtggatga
     1621 aattccggtt ccggaagatg cttgggtcga ttgggacgca acgactcaga ccttcattac
     1681 agtgggtcaa aaatacccag atggcaccac tgccaaggtt aagagcacgg tggtgtaccc
     1741 tgccgatttg tttgaaaccg tcaaatggca tgatggcagc ccaatctctg tcgcagattt
     1801 tgtcatgtcg ttcgtcattt tccttgatcg cggtaagcct gaaagtgcag tctatgatga
     1861 agcggcggct ctttccgtgg atgctttcct accttccttc aaaggttggc gtatcacctc
     1921 taccgccccg ctcaccattg aatactactc tgatgcgtac tctgctgatg ctgagttgaa
     1981 tgttgctccg ttgtggcccg gttccccgtc agggctttct ggtgagaata gctggcaaat
     2041 tttggcgatc tccaatctgg cagaagctgc tggcgaattg gcttactctt ccgataaggc
     2101 agatgctaat agcattgagc aaatgagctg ggtaggcgga ccaagccttg agatcctcaa
     2161 gaagtatttg gaccaggctc agaccgaaag ctatgttccg tatgaagcga ccttgggtca
     2221 gttcctgact ccggaagaaa tcgctcttcg ctatgaaaac ttctccaaat ggtatgccga
     2281 ccatggacat ttctgggt
//
LOCUS       DAOREF010000530         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35479_length_2294_cov_4.414024, whole genome shotgun sequence.
ACCESSION   DAOREF010000530 DAOREF010000000
VERSION     DAOREF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35479_length_2294_cov_4.414024"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKI33_10930"
     CDS             complement(<1..424)
                     /locus_tag="PKI33_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37572.1"
                     /translation="MAKQIADASFALPSQSPYYGPGGIPFAYPPLGLYVLAICIKLTG
                     KYYIFLRFFPPLYSLFAVGLTFLLAQKFFKQPFLSMMVAILTATSVDLYVAHTWSSGV
                     VRALAFIFTILVLYFYSPNPDERTRKDIFLAGLFFGLAG"
     gene            complement(559..2112)
                     /locus_tag="PKI33_10935"
     CDS             complement(559..2112)
                     /locus_tag="PKI33_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37573.1"
                     /translation="MKNILGVRFKLFLLFFAVGTGFTFAIGWLTQFHVTLSDYWNVLY
                     YGRHMTLSQPESLYNGFYPFGYAFLIGQMPLTYVLPLSYLLNALLSGLLVASVSTLVA
                     YTDSIPATVIAFYSSIAAPFVFQNANTLGPDIGAAAFIAFAVFLLCHKWLSREDKPLS
                     DTQLILAGVSLGLSFLWRTHAAVAIIAILAGYILIAGIRPFRPLILLAASLAGVAGIQ
                     IIVNLVSGHGPLETAQAFNLYKYFYGVNWTNPVGPEVVKDFSLIRTVTNDPTAAFNLY
                     KPFFLYFISHTWAALLAFILLPKGRFSRFSLFSFVFIVLYAIPISLSDSPRAPAVLMS
                     VYLPSLAVLLAVLIEAIKKYFPSIRLGEWIGIGVFIAVAFPTFYSWAVYDVGIAQAAI
                     AERKVLSVIEQTLRTKGLTSSDEIFSDRYDFYTPNTMPYHARGIEKWSVDWFWGMSET
                     YPLLPNDTWESFATACKEEGIRFLVLGPNSYLRGDFFGQIYTDDVNLDALGLQFIGQR
                     GNKRLYEFK"
     gene            complement(2112..>2294)
                     /locus_tag="PKI33_10940"
     CDS             complement(2112..>2294)
                     /locus_tag="PKI33_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37574.1"
                     /translation="QGFNDLLVGLASALGSLESGFIFASLGYNMMAYVSATIALIPLL
                     VVVVWMMRTPTKLKPA"
BASE COUNT          808 a          474 c          520 g          492 t
ORIGIN      
        1 gcccagctag cccaaaaaac agccccgcta aaaagatatc tttccgcgtc cgttcatcag
       61 gattaggaga gtagaaatac aaaaccaaga ttgtaaaaat aaaagcaaga gcgcgtacaa
      121 cacccgaaga ccaagtatgt gctacatata agtccactga ggttgcagtc aagatcgcga
      181 ccatcatcga aaggaaaggc tgtttgaaaa atttttgagc gaggaggaag gtcaacccaa
      241 ccgcgaagag actataaagt ggtgggaaaa agcgaaggaa gatgtaatac ttacccgtca
      301 acttgataca tatagcaagt acatacaagc ccaatggtgg ataggcaaat ggaatgccac
      361 ctggaccata atatggagat tgactgggca aagcaaagct cgcgtccgca atttgttttg
      421 ccatttgtgt aaacaaccca gcatacccca ttgggaagtc atgtaaaaat gcgttataaa
      481 aaagtaaagg aagagaaccc aacattgcaa tcaaaaaagc aattttggaa tcagtccatt
      541 ttaattttgt aatccagatc atttgaactc gtacaatctt ttattgcccc tttgcccaat
      601 aaattgcaag cctaaagcat caaggttaac atcatcggta tagatttgac caaagaaatc
      661 tccacgaaga tagctattcg ggccaagaac gagaaaacga attccctctt ctttacaagc
      721 agtggcaaat gattcccaag tgtcatttgg caataatgga tatgtttcac tcattcccca
      781 gaaccaatcg acggaccatt tttcgatccc tctagcatga taaggcatgg tattcggggt
      841 ataaaaatcg taccgatcag agaaaatttc atcagaagaa gtcagacctt ttgtgcgaag
      901 agtctgttca ataacagaaa gaactttccg ttcggcaatg gcagcctgtg ctataccgac
      961 atcatataca gcccagctat aaaatgttgg aaaggcgaca gcaataaata ctccaattcc
     1021 aatccattca cccaaacgaa tagagggaaa atatttctta atagcttcga tcaaaacggc
     1081 taacaaaacg gctaacgatg ggagatacac gctcatcaaa acagctggtg cacgagggct
     1141 atcgctaagc gagatcggaa ttgcatacaa aacaataaaa acgaaggaaa ataaagaaaa
     1201 gcgggaaaaa cgccctttcg gcaaaaggat aaatgcaagc aaagcagccc aagtatggga
     1261 aatgaaatac aagaaaaatg gtttatataa attaaaagct gctgtagggt cattcgtaac
     1321 tgttcggatt aacgaaaaat ctttgaccac ttctggacct actgggttcg tccagtttac
     1381 gccatagaag tatttgtaaa gattaaaggc ttgggcggtc tcaagcggac catgcccaga
     1441 aacgaggttg acgataattt gaatgcccgc tacacctgct aaagaggctg ctagcagaat
     1501 cagcgggcga aatgggcgaa ttccagcgat gagaatgtac ccggcaagaa tagcaatgat
     1561 ggcaacggca gcatgcgtcc gccagaggaa ggagagaccc agagagacac cagccagaat
     1621 aagctgtgta tctgatagtg gtttatcttc tctacttaac catttatgac atagcaaaaa
     1681 gacagcaaat gctataaatg ccgcggctcc aatatcgggt ccgagggtgt tggcattttg
     1741 gaagacaaat ggagcagcaa tagaagagta aaacgcgata accgtggcag gaatggaatc
     1801 ggtgtaggca acaagtgtcg aaacagaggc aaccaaaagt ccagaaagaa gcgcgtttaa
     1861 taaataagaa agcggcagga cataggtcaa cggcatctgc ccaatgagga aggcgtatcc
     1921 aaagggatag aaaccgttgt acagtgactc tggctgcgag agagtcatgt gacgcccgta
     1981 atacagaacg ttccagtaat cgctaagagt tacgtggaat tgagttagcc agccaatcgc
     2041 aaaggtgaac cctgtgccaa cagcaaagaa taacaaaaat aatttaaagc gtacaccgag
     2101 aatatttttc atcaagcagg tttgagtttt gtgggggttc tcatcatcca aacaacgacc
     2161 accaaaagcg gaatcaaggc tatggtagcg ctgacatatg ccatcatgtt atagccgagt
     2221 gaagcaaaga taaaaccgct ttccaaactt ccaagagcag atgcgagacc aacgagaagg
     2281 tcattgaagc cttg
//
LOCUS       DAOREF010000531         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35515_length_2293_cov_3.933870, whole genome shotgun sequence.
ACCESSION   DAOREF010000531 DAOREF010000000
VERSION     DAOREF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35515_length_2293_cov_3.933870"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..450)
                     /locus_tag="PKI33_10945"
     CDS             complement(76..450)
                     /locus_tag="PKI33_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNM37575.1"
                     /translation="MLGELSARQTTGDLTPSQFAVLEALYHIGSMTQGEVSSKVLKST
                     SNLTTVIDNLERDGYVRRERDAEDRRVIHVHLTEAGTSKIEAVLPNHVGALVEIFSVL
                     SPAEQESLGELCKKLGKSKESA"
     gene            complement(679..1239)
                     /locus_tag="PKI33_10950"
     CDS             complement(679..1239)
                     /locus_tag="PKI33_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNM37576.1"
                     /translation="MRIAKHFRKDQETLQRFLDVFGAGSAMLGGGNKNVQPGFFIHGS
                     TFIHEYVDGNFFRKEELLMKTLEDAGFPVNSGVIGNMKDEHAKCRQAADLLSKAASDW
                     QGGDAGARIEVGWAASEYTSTLRQNLDRLKNLVFPLLDQNIPPEDESAIIDGLNQIAL
                     ENSKEIEAGKYTKLLETLEEELSDWK"
     gene            complement(1492..2046)
                     /locus_tag="PKI33_10955"
     CDS             complement(1492..2046)
                     /locus_tag="PKI33_10955"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNM37577.1"
                     /translation="MNNHSQEQFKNLFLEKMETFTRNEWELLRHIMDRRHVTRNVNKE
                     FDDQLTFGQRLADNVAKFGGSWTFIIIFGLILLTWIFLNSVVLARLNDAFDPYPYILL
                     NLFLSMLASIQAPIIMMSQNRQATKDRMDAAHDYEVNLKAEMEIETLHQKLDELRDKQ
                     WAELVEMQQEQIRLLTKLLEEKNK"
     gene            complement(2039..>2293)
                     /locus_tag="PKI33_10960"
     CDS             complement(2039..>2293)
                     /locus_tag="PKI33_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37578.1"
                     /translation="VLPFVLGMSKLNTKKFVLLDFIGAFLWAITFGLAGHFIGHVMTL
                     IFDDVKEHELPVMIGIALIGIGIWLYRRNNNKDKDGKYHE"
BASE COUNT          584 a          593 c          492 g          624 t
ORIGIN      
        1 agcgatgcga ggcaacggtc acaccatccc aactcgtcac cgcctgtgta atgcgcgcct
       61 gcgctccctt cacgctcatg ccgactcctt acttttgcct aacttcttgc ataactcacc
      121 aagtgattcc tgctccgccg gacttaacac actgaagatc tccaccaatg ccccaacatg
      181 attgggaagc accgcctcga tcttgctcgt gcctgcttct gtcaaatgaa catggatcac
      241 tctgcggtct tctgcatccc tctcgcggcg gacgtatcca tcacgttcga ggttgtcgat
      301 gacggtggtg aggttgctcg tgctcttcag caccttgctg ctgacctcac cctgggtcat
      361 cgagccgatg tgataaagcg cctccagcac agcaaactgc gaaggggtca ggtcgccggt
      421 ggtttggcgg gcagatagtt ccccaagcac cgagttggta cagcgggtga gcttgatgaa
      481 cgtttccagg gtgcgcctct cggcgcggga gccttcaaaa tgagtgggca tatcaaatct
      541 ctttcgacgt caaatagttt gatatcgaaa tattatcgga aaaccgcccc gccgtcaaga
      601 aaatcttccc tagccgtttg ggtaggaatt aaaaacccgc cttggcgaag gcgggtccat
      661 gggttcgagg ttggatgatt acttccagtc gcttagttct tcctcgagag tttcgagcag
      721 tttggtgtat ttccctgcct cgatctcctt gctattctca agtgcaattt gattcaaccc
      781 atcgatgatc gcagactcat cttccggcgg gatgttttga tcaagcagtg ggaaaacgag
      841 atttttgagg cggtccaaat tctggcgcag ggtggatgtg tattcgctcg ccgcccaccc
      901 cacttcaatt cgggcgccag catccccgcc ctgccagtca ctggcggctt tggaaagcag
      961 gtcggcggct tgcctgcatt tcgcatgttc atctttcata tttccgatca cgcccgagtt
     1021 gacgggaaac ccggcatctt cgagagtttt cataagcagt tcttccttgc gaaagaaatt
     1081 tccgtctaca tactcatgga tgaaggtgct gccatggatg aaaaaacccg gttgaacatt
     1141 tttattgcct cccccaagca tggcagatcc tgcgccaaac acatctaaaa accgctggag
     1201 agtttcttga tctttccgaa aatgtttcgc aatacgcata tgggtttcct gtcagtagaa
     1261 cgggactttt tctttgcttt aaaattttac cgccctttaa tagaaacaaa cagcaatgaa
     1321 cccgtgcaat tgtatgctaa tttagccaac tgatctcatt ccaaaccaag acgcaaacaa
     1381 ccccaaaagg acagagcgcc agtccgctgg cagccagtaa ggggctatca cgagttttcc
     1441 cgatggaaac aaaacaggtc agagttgctc tgacctgttt tactcaaacg tttatttgtt
     1501 tttttcttcc aacagtttcg tcaacaatcg aatctgttcc tgttgcattt ccaccagttc
     1561 tgcccattgt ttatcgcgca gttcatctaa tttctggtgc aatgtttcga tctccatctc
     1621 tgcttttaag ttcacttcgt aatcatgtgc tgcatccatc cggtctttcg ttgcctgccg
     1681 gttttgagac atcataataa taggagcctg aatcgacgcc aacatggaaa gaaagagatt
     1741 tagcaaaata taagggtatg ggtcaaaagc atcattcaac ctagcaagca caaccgagtt
     1801 gagaaaaatc caggtcaata agatcaagcc aaagataatg atgaacgtcc atgatccgcc
     1861 aaatttggca acattatccg cgaggcgctg accaaaggtt agttggtcat caaactcctt
     1921 gttgacgtta cgggtcacgt gtctgcgatc catgatgtgg cgaagcagct cccattcatt
     1981 tctggtaaat gtttccattt tttctaaaaa caggtttttg aattgttcct gagaatgatt
     2041 attcatggta tttaccatcc ttatctttat tattgttacg gcgatacaac caaatgccaa
     2101 ttcctatcaa agcgatcccg atcatcacgg gcagttcatg ctctttcaca tcatcaaaga
     2161 tcaatgtcat cacatgccca ataaaatgcc cagccaaccc aaaagtaatt gcccacaaaa
     2221 aagcacctat aaaatctaat aaaacaaatt tctttgtatt cagtttggac ataccaagca
     2281 caaaaggtaa tac
//
LOCUS       DAOREF010000532         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35585_length_2291_cov_4.564848, whole genome shotgun sequence.
ACCESSION   DAOREF010000532 DAOREF010000000
VERSION     DAOREF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35585_length_2291_cov_4.564848"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1202
                     /locus_tag="PKI33_10965"
     CDS             264..1202
                     /locus_tag="PKI33_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37579.1"
                     /translation="MKDAPYFQPVDIDLVSLGDETVLIEGHAVSVIRHQYDEYVQMVE
                     ARFLLKNPFDSSTLQERSKIEDALKSKYIPVEHRQSGMFEEYTILLVDEVKQAPDKWI
                     DKNALDISRFIRSQRDTFDKNEIAEILMSRTRYSASELTLVDWEGAVIVAPNGDYDSD
                     VVLLKIGNYQLLRYRMLDHSIENLLDKINESFFENKRRPRATRGLIRQIAEHKLEVML
                     DFERTEQTLLLIGDWYTAKLYEAIQSEFYLKDWKESLRAKLDNLESIMQTIKDNLSVS
                     WENLMDRIQLLLWMVMLIGYLYLYLLDAKWITIPSN"
     gene            1224..2213
                     /locus_tag="PKI33_10970"
     CDS             1224..2213
                     /locus_tag="PKI33_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37580.1"
                     /translation="MSKNISFMGVLLSLVLFLFIGLYFGQKSILPLGPDAYASCAVSM
                     DHVLDSGKGDVYNFSGVTNFEEPAFYYLASYDVEESTITDPILKTVPKDLQDEQHDTV
                     LQQEAWSIFTTLIPGQNRQMVSQFNVFTDGYSNTLAAVDQTENDLSQWMLEVDVADLE
                     DKDALIYTMVHEYAHLLTLNSSQVTPDADTYKDPLNPELQKEKAAACSTYFVGTGCTH
                     PDSYMNAFYNRFWADISDEWQQVDALQYGAEDLVPYYNGLYAFYLKHKDRFVDDYAVT
                     HPAEDIAESFTYFVFNPKPVGNSIRDQKLAFFYEYPELVQLREDILSGACSMK"
BASE COUNT          625 a          479 c          520 g          667 t
ORIGIN      
        1 ccgatcacat tttcccaatt gcgagatcta agcgctcggt tgcgccctcg taatacatag
       61 agcttctcct tatagtgtaa gtcccggcac tcggttagag agaagttatt ttgtacgggt
      121 gtcgggtctg tattggatga agataacaac ttgcgttacc tataaccctc ctgtgctaca
      181 cttatggcat ggagatttgt tacttaatag cttttcctga tacagaacat aaagttgcac
      241 cagtaaaaga gtcaatcaag ggcttgaaag atgcacccta ctttcagcct gtagatatag
      301 accttgttag cctgggggat gagaccgttt tgattgaagg tcacgccgtt tctgtgattc
      361 gccatcagta tgatgaatat gtgcagatgg tggaagcacg tttcttactg aagaacccat
      421 tcgattcatc aactctgcaa gagcgcagca agatcgaaga cgcacttaag agcaaataca
      481 ttcccgtaga acaccgccag agcggtatgt tcgaagaata tacgattctt ctggtggatg
      541 aggtgaagca ggcacccgat aaatggatcg ataagaatgc cctcgatatc tcccgtttta
      601 tccgctctca gcgagatact tttgataaga atgagattgc tgagattctc atgtcgcgga
      661 cgcgttattc tgccagcgag ttgaccttgg tggattggga aggggctgtc attgttgcgc
      721 ccaacgggga ttatgattcg gatgttgtct tattaaagat tggtaattat caattgttgc
      781 ggtatcgcat gctggatcat tccatcgaga acttgttgga taagatcaat gaatctttct
      841 ttgagaacaa acgtcgcccc cgtgccaccc gcgggcttat ccgccagatt gcggaacaca
      901 agcttgaagt catgcttgat tttgaacgca ccgagcagac cttgctcctg atcggagatt
      961 ggtatactgc caaattgtat gaagccattc aatcagagtt ttatctcaaa gattggaagg
     1021 aaagtctgcg tgccaagctc gataatttgg aaagcatcat gcagaccatc aaagataatt
     1081 tatcggtttc gtgggaaaat ctgatggata ggattcagtt attgctttgg atggtgatgc
     1141 tgatcggtta cctgtatctc tatcttttag acgcgaaatg gatcacaatc ccttcaaatt
     1201 aatggtatct ccgcggaggc ggcatgtcga agaacataag ctttatgggt gttttacttt
     1261 cgctggtttt atttcttttt atcgggctgt attttgggca aaagagtatt ttgcctcttg
     1321 gacctgatgc atatgcatct tgtgctgttt ccatggatca cgtgcttgat agtggcaaag
     1381 gcgatgtgta caacttcagc ggagtgacta attttgaaga accggcattt tattatcttg
     1441 cctcttatga tgtagaggaa agcaccatca ccgatcctat cctaaagaca gttccaaaag
     1501 atttacagga tgagcaacac gataccgtgt tgcaacagga agcatggagt atttttacaa
     1561 ccctaattcc tggtcagaat cgtcaaatgg tatctcagtt taatgtcttc accgatggat
     1621 attcaaacac cctggcagcc gttgaccaaa cagaaaatga cctctcacaa tggatgctag
     1681 aagtggacgt tgctgacctc gaagataaag acgcccttat ctatacgatg gttcacgaat
     1741 atgctcactt gttgaccctg aattcatctc aggtcacgcc agatgcagat acctataaag
     1801 acccactcaa ccctgaattg caaaaagaaa aagccgcagc ttgttctact tattttgtag
     1861 gcacaggttg cacccatcct gattcataca tgaatgcttt ttacaatcgt ttttgggcag
     1921 atatttcaga cgagtggcaa caagtggacg cgctccaata tggggcagaa gacctagtgc
     1981 catattacaa cgggttgtat gcgttttacc ttaagcacaa agatcgcttt gtagatgatt
     2041 atgctgtaac gcaccccgcc gaagatattg ccgagtcttt tacctatttt gttttcaacc
     2101 ccaaaccagt tggcaattcc attcgtgacc aaaagctcgc cttcttttat gaatacccag
     2161 agctggtgca gttgcgtgaa gatattttga gcggcgcttg ctccatgaaa taatgaggat
     2221 tttgtgtaga aaatatcgta atgagactgt taattcgata aaaattcctt atggtatcct
     2281 tggaaaaaaa t
//
LOCUS       DAOREF010000533         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35692_length_2287_cov_5.586022, whole genome shotgun sequence.
ACCESSION   DAOREF010000533 DAOREF010000000
VERSION     DAOREF010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35692_length_2287_cov_5.586022"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PKI33_10975"
     CDS             <1..139
                     /locus_tag="PKI33_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HNM37581.1"
                     /translation="RSFSQRATNRTFKPNLQKVSVMEKGRLVQKTLCTKCIKTLGKSA
                     A"
     gene            complement(225..890)
                     /gene="rpe"
                     /locus_tag="PKI33_10980"
     CDS             complement(225..890)
                     /gene="rpe"
                     /locus_tag="PKI33_10980"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNM37582.1"
                     /translation="MTHYLLAPSIIASDFAHIADEVAAIESAGADWIHVDVMDGHFVP
                     DITIGPLFVKGLRRVAKLPLDAHLMISEPEKYIPAFAEAGVNNITVHVETCPNLPKVI
                     EQIKSHGCTAGVTLNPATLASAIEAVLPLVDLVLVMSVVPGFSGQKFMPEMINKVAEI
                     RAKLNVLGSHAHLEVDGGINAETLPLMKNAGANVFVTGNAAFKHPQGSGAGVKALKEI
                     LNK"
     gene            complement(938..2077)
                     /locus_tag="PKI33_10985"
     CDS             complement(938..2077)
                     /locus_tag="PKI33_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNM37583.1"
                     /translation="MTESALLNDRYQLDELLGSGGMANVYRARDVVLDRPVAIKVLRK
                     EYSTNENFQKQFWLEARSAANLSHPNIVTVHDFGAADDLLYIVMELVPGKDLKQLVRE
                     RGRFPYQQGIPLVIQACAGVGYAHRAGLVHCDIKPHNMLVSKDMRLKVTDFGIARALA
                     TIKPGERNDVVWGSPLYFAPEQAAGEAPTPASDVYSLGVVLYEILTGTTPFTASTADE
                     LARLHISARPLPISEYVPDVPPALEEIVMKVLSKEPAARYRTADQLGRVLQKFGTEPA
                     APPKPKQEPVSSPPVITIKQDIAERLSPPSQPRPQPEPAPQYYQPQYPPQSIQPEPAP
                     TYREEEVYTEPIDWLAVGLGLMAVLAWGGLIPFGLMIYLSYFPPS"
BASE COUNT          564 a          544 c          613 g          566 t
ORIGIN      
        1 ccgctccttt tcccagcgtg ccaccaaccg cacgttcaaa cccaacctcc agaaggtttc
       61 ggtgatggaa aagggtcgcc tcgtgcagaa gacgctgtgc accaagtgca tcaaaacgct
      121 tggcaaatca gcggcataag gtttcgttcc ttttgactca agaatcgcgg cgcgtaagcg
      181 ccgtgatttt tgttttaaca ctgaaaaggc aggttgaccc tgccctactt gtttaatatt
      241 tctttcaatg cctttacccc tgccccacta ccttgtgggt gtttgaatgc agcattgcct
      301 gttacaaaaa cattggctcc ggcgtttttc atgagcggaa gagtctctgc attaataccg
      361 ccatccactt cgaggtgagc gtgtgaacca agtacgttca acttggctcg aatctctgcc
      421 actttgttga tcatctcagg cataaacttc tgccctgaaa aacccgggac gacactcatt
      481 actaatacca agtccacaag aggaagaacc gcctcaatgg cagaagcgag ggttgctggg
      541 ttcaaggtca caccggcggt gcagccgtga gacttgatct gttcgatcac tttgggcaaa
      601 ttggggcaag tctctacatg gacagtgatg ttattgacgc ctgcttcagc aaatgcaggg
      661 atgtatttct ccggttcaga gatcatgagg tgggcatcta gcggaagctt ggcaactcgc
      721 ctcaaccctt tgacgaataa cggtccgatg gtgatatcgg ggacaaagtg tccgtccatg
      781 acgtcgacat gaatccagtc ggcgccggca gattcaatgg cggcaacctc gtctgcgatg
      841 tgcgcaaaat cagaagcaat aatagaaggg gctaaaagat agtgagtcat tatcaaaaag
      901 acctgactag tttaggtgaa ctgtcaggtc tgtccattta acttggtggg aagtacgaga
      961 ggtagatcat cagcccgaag gggatgagtc cgccccatgc gaggacagcc atgagcccaa
     1021 gcccaaccgc cagccagtca ataggttcgg tgtaaacctc ttcttcacga taggtaggag
     1081 caggctccgg ctgaatcgat tgaggcgggt attgcggttg ataatattgc ggagccggtt
     1141 caggctgcgg tcgaggttga ctcggaggtg aaagtcgttc ggcaatatcc tgtttgatgg
     1201 taattacagg cggcgaggaa acaggttcct gcttcggctt aggcggagcc gcgggttcgg
     1261 ttccaaattt ttggaggacg cgccccagtt ggtctgctgt gcgatatcgt gccgccggtt
     1321 cctttgaaag taccttcatg acaatttctt caagggcggg cggaacatct ggcacgtatt
     1381 cgctaatagg cagcggacgt gcggagatgt gcaaacgtgc cagttcatcg gcagtggagg
     1441 cggtgaaagg ggtggttcct gtcagaattt catagagcac aacgcccaac gagtacacgt
     1501 ctgaagctgg ggttggcgcc tcgcctgcgg cttgctcggg cgcaaagtag agcggagagc
     1561 cccatacgac atcgtttcgt tcaccaggtt tgatggttgc caacgcacgt gcgataccaa
     1621 aatctgtgac tttgaggcgc atatctttgg aaacaagcat attatgcggc ttgatatcgc
     1681 agtgaaccaa gccagcgcgg tgcgcatacc ctaccccagc acaagcttgg ataacaagcg
     1741 gaatcccctg ttgataaggg aaacgtccgc gctctcgtac gagttgcttg aggtctttgc
     1801 caggaacaag ttccatgacg atgtagagca aatcatcggc agcgccaaaa tcatgaactg
     1861 ttacaatatt ggggtgcgaa agattcgccg cggaacgcgc ttccagccag aattgttttt
     1921 gaaaattctc gttggtggaa tattctttgc gtaagacctt aatcgcaaca ggacggtcta
     1981 gcaccacatc tcgcgcgcga tacacgttcg ccatgccgcc agagccgaga agttcgtcaa
     2041 gttgatatcg gtcgtttagt aatgcacttt cggtcattgt ggtgagatta taccgtgagt
     2101 tctacataga ggcacggaga tagaaaatcg atattttggg gagtttatat gtttagttgt
     2161 cttaacaggt ctgttaaata aagttgtcat aacgattttc acccgttatt cgtaaatcgt
     2221 ttacaatatg actgtttagg agatccacca tgctaggcga accaatcctt gtcatgctcg
     2281 ttgaaga
//
LOCUS       DAOREF010000534         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35754_length_2285_cov_4.720179, whole genome shotgun sequence.
ACCESSION   DAOREF010000534 DAOREF010000000
VERSION     DAOREF010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35754_length_2285_cov_4.720179"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKI33_10990"
     CDS             <1..477
                     /locus_tag="PKI33_10990"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNM37584.1"
                     /translation="SVIWTNLALLGFVGWTIHLIGWQAYLLVELPILLMACGAGVWLF
                     YVQHNFDPTYWERHKDWSFFNAGMDGSSFYKLPKVLQWFTGNIGFHHIHHLSPKIPNY
                     KLEECHNENPIFQIEPLTFRDSLKSLYFRLWDEKEKVLVGWNAIKKYKMQTQRNQM"
     gene            complement(531..1991)
                     /locus_tag="PKI33_10995"
     CDS             complement(531..1991)
                     /locus_tag="PKI33_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNM37585.1"
                     /translation="MATKNSKTKPKAPTKSAAAKQVEVQKEAANPKVFGYLPDDNPPI
                     AAMLSLGFQQFLTMFPATVLVAILTKFDVGVTLLASGLGTIIALLVSKRKIPMYYGSS
                     FSYIAVIITVMSQFAPDCFNDATQVYCADGVKIVQVGIILTAVFEILVGLLIMRVGKS
                     ALDRVLPPIVTGSMAMVIGIALANAALGNAGNWAAPELATATWTVAFLTLLATVLFSV
                     YLQGKGLIGMLPILLGAIFGYLVSIPFGLVTFDKVANAAWLEMPKFTFPAFTNPAAWG
                     MALSVALIFIATVPESTAHLYQMSLYIDELAAELGRKPFNIKELIGLNLVADGADDLV
                     VGFLGGAPGTNYGENNSLMAITRNYSTAVLMTAGAMAIVLGFFGKLVALVNTIPAAVV
                     GGLSIYLFGVIGMQGVALIQSEKVNLFDPKQLAVGAIILICGIGGNLSLPNGVFPFPI
                     PYLFPSGIPAIVFAAIAGILVNTIFVLFKAPEVRGG"
BASE COUNT          593 a          589 c          600 g          503 t
ORIGIN      
        1 tctgtgattt ggacgaacct tgccttactc ggctttgttg gctggacgat tcacctgatc
       61 ggctggcagg cgtatttgct ggtggaactt cccattttgc tcatggcgtg cggcgcgggt
      121 gtgtggctgt tctacgtgca gcacaacttc gaccccacct actgggaacg ccacaaagat
      181 tggagcttct tcaatgccgg tatggatggc agttccttct ataagctccc caaggttttg
      241 cagtggttca cgggcaatat cggcttccat catattcacc acctcagccc gaagatcccc
      301 aactacaagt tggaggaatg ccacaacgag aaccccatct tccaaatcga gccgctcacc
      361 ttccgcgaca gtttgaagtc gttgtacttc cgcctgtggg atgagaaaga gaaagtgctg
      421 gtcggctgga atgcgatcaa gaaatacaag atgcaaacgc aaagaaacca aatgtagtta
      481 aacaaaaagt gactatcaaa acgacagtca ctttttttgt ttcaaacgga ttatccgccg
      541 cgtacttcag gggctttgaa caagacaaag atggtgttca caagaatacc ggcaatcgca
      601 gcaaagacaa tggcagggat gccactgggg aaaagatacg gaattgggaa ggggaacacc
      661 ccattgggca gcgagagatt cccaccgatg ccgcagatca agatgatcgc accaaccgca
      721 agctgtttcg ggtcgaagag attgaccttc tccgattgaa tgagtgccac gccttgcata
      781 ccgatcacac cgaagagata gatgctcaag ccgccgacga ctgccgccgg aatggtgttg
      841 accaacgcca ccagtttccc gaagaagcca agcacaattg ccattgctcc tgcagtcatc
      901 aacactgcgg tggagtagtt gcgagtgatc gccatgagcg aattattttc gccgtagttt
      961 gtgcccggag caccgccaag gaagccgacc acgagatcat ctgcgccatc ggcgaccaga
     1021 ttcaacccga tcaactcttt gatgttgaaa ggtttgcgcc ccaattcagc ggcgagttcg
     1081 tcgatgtaca aactcatctg atacaggtgc gccgtcgatt cagggacggt agcaatgaag
     1141 atcagtgcca cgctcaatgc catgccccaa gcagcgggat tcgtaaatgc cgggaaggtg
     1201 aacttgggca tttcgagcca ggcggcattt gccactttat caaaggtcac caacccaaac
     1261 ggaatggaaa ccaaatatcc gaagatcgcg cccagcaaaa tgggcaacat gccgatcagc
     1321 cctttgcctt gcaggtagac cgagaacaac accgtggcga gcaaggttag gaaggcaact
     1381 gtccatgtgg cggtggcgag ctcaggggct gcccaattgc cggcattgcc gagcgccgca
     1441 tttgccaggg cgataccgat aaccattgcc atcgaaccgg tcacaatggg cgggaggacg
     1501 cggtcaagtg cgcttttacc tacacgcatt attagcaaac cgaccagaat ttcgaacact
     1561 gccgtcaaaa tgatgcccac ttgcacgatc ttcacgccat cggcgcaata gacttgggtc
     1621 gcatcgttga aacaatcagg cgcgaactgg ctcatcaccg tgatgatgac ggcgatgtac
     1681 gagaacgacg agccgtaata catcggtatt ttgcgcttcg aaaccagcag cgcaataatc
     1741 gtccccaagc cggatgccaa caacgtcacc cccacatcga acttggtgag gatcgccacc
     1801 aacacggttg ccgggaacat cgtcaggaat tgctggaagc ccaggctgag cattgccgcg
     1861 atcggcggat tgtcatcggg cagatagccg aagactttgg gattcgccgc ctctttctgg
     1921 acttcgacct gtttcgcagc cgcagacttg gttggtgcct ttggctttgt cttgctattt
     1981 tttgttgcca tgtgcattct cctcgatctg agataaaaaa agagagccgc tacaagcgac
     2041 tctccgattc acataataga gacagccatt ccaaagaagt gttgatgcgg gaagggatgt
     2101 cgaaattttt ctgttaacat tttggcgaat tttacgccta tttaagagag tgtcaaggaa
     2161 tttgagcgct ttgggcgtgg aaaatttttc agaaaaccga agcaactgcg ggcggggaaa
     2221 ggaagcgctg attaagagca tctccactta tgatggttaa gtcgtgggtg tataatccag
     2281 ccgat
//
LOCUS       DAOREF010000535         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35762_length_2285_cov_3.311659, whole genome shotgun sequence.
ACCESSION   DAOREF010000535 DAOREF010000000
VERSION     DAOREF010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35762_length_2285_cov_3.311659"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKI33_11000"
     CDS             complement(<1..775)
                     /locus_tag="PKI33_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNM37586.1"
                     /translation="MKRIEQLTFTRFIIIILVLFAHDILGPYLNPFLFFPLEALLRSG
                     STGVSYLFILSGFVMTIVYYKPGEKFDHFSFWRNRLIRLYPLYIFAFLLTCIYYYDSL
                     FRIKPEKILANIFILQSWIPKYAQSFNYVAWSMTVEVFFYLFFPFFLVWTYRQSTRKL
                     INFSLVIWIISTTIFQILWIGYIDQYRDFILYFPLFYFNSFVLGVVGGIWYIREGRNE
                     TISTEKRFAVLTASFLVIATYTVLSMDFFPSWPHLIQPIT"
     gene            complement(839..1825)
                     /locus_tag="PKI33_11005"
     CDS             complement(839..1825)
                     /locus_tag="PKI33_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNM37587.1"
                     /translation="MKSTYPWIFATLLFLTLALKPSEKAGAQPEAFSPNQTAQEMIEK
                     VNTLRASNGLEPYVSDAILMKIAQSHADYIASTGVLTHFDKNGKRPYQRALDAGYAVG
                     GNLAAGGLLTEAIFSGSGVPDNAVIAAWQANEADSVALLSSSYEDVGVGISAANGTTY
                     YVLVAGSQNDSGISVTPTFVSSVAPLGTGTNIPNTPKPDGQIYHVVRKNEALWSVAIL
                     YNTTIEELKLLNGLATDEIFEGQQLLVRAANTQTPTPSPVPVTATLGIPTSTATLPVT
                     PTKTSTPTPVPTPPTSLKSGGMVVGGITLVALLAAGLGAFLGKAKKNPNEGE"
     gene            1912..>2285
                     /locus_tag="PKI33_11010"
     CDS             1912..>2285
                     /locus_tag="PKI33_11010"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNM37588.1"
                     /translation="MPGFTPGRRYQPPFLPFKRQPFKKRAFAAIERAVKGPLFGCRMC
                     GNCLLQETALICPMECPKGLRNGPCGGSTPERCYVDESRPCIWYKIYDRSFKLGREAL
                     LLEVLPPMDWNKAGTDAWSDVF"
BASE COUNT          708 a          499 c          518 g          560 t
ORIGIN      
        1 ctgtgatagg ttgaatcaag tgaggccaag aaggaaaaaa atccatactc aatacggtat
       61 aggttgcaat tacgaggaaa ctggcagtta atacggcaaa gcgtttttcg gtactgatcg
      121 tctcattacg tccttctcgt atataccaaa taccacctac tacgcctaaa acaaaagaat
      181 tgaaataaaa caatgggaag taaagaataa aatctcgata ctggtctata tacccaatcc
      241 acaaaatctg aaagattgtg gtgctaataa tccagataac gagcgagaaa tttatcaact
      301 ttcgagtgga ttgacgatac gtccaaacca aaaagaaagg aaaaaataaa tagaaaaata
      361 cttctacagt catggaccat gctacatagt tgaaagactg agcatatttt ggtatccaag
      421 attgaagaat aaaaatattc gctaatatct tttcaggttt aatcctgaaa agagagtcgt
      481 agtaatagat acaagttagg agaaacgcaa aaatatagag aggatagaga cgaataagtc
      541 gattacgcca aaaactaaaa tgatcaaact tttcacctgg tttgtaataa acaattgtca
      601 tcacaaaacc tgacaagatg aaaaggtaag agacacccgt tgagccagaa cgaagcaatg
      661 cctccaaagg gaaaaacaaa aaagggttta agtaaggtcc aagaatatca tgcgcaaata
      721 gcaccaaaat aataatgata aagcgcgtaa aagttaattg ttcaattcgt ttcatcaagc
      781 ctcagattca aagtccacca attctactag aaaatttgga taccccaatg acggcgtcct
      841 attcgccttc attggggttc tttttcgctt ttcctaaaaa cgcacccaaa cccgcagcta
      901 acaaagcgac cagagtaatt ccacccacca ccatcccgcc gcttttcaaa ctcgttggtg
      961 gggttgggac tggtgtgggc gtagaagttt ttgtcggcgt gacaggtaag gttgctgtag
     1021 aggtaggaat tcccaaggtt gccgtaactg gaacaggtga aggcgttggc gtttgagtat
     1081 ttgctgctcg cactaaaagt tgctgccctt caaaaatctc atcggtagcc aagccgttta
     1141 ataactttag ctcttcaatg gtggtgttgt ataaaatggc aacactccac aaggcttcat
     1201 ttttacgcac cacgtgataa atctgcccat caggcttggg tgtgtttgga atattcgtcc
     1261 cagtccctag cggggcgaca gaactgacaa aggtcggcgt gacagaaata ccagagtcat
     1321 tttgggagcc tgccactaaa acatagtatg tggttccatt ggcagcggaa atgccaaccc
     1381 cgacatcttc ataagaggat gaaagcaaag cgacagagtc cgcctcgttt gcttgccatg
     1441 cggcaatcac agcattatct ggaacaccag aaccggagaa aatagcttcg gttaacaagc
     1501 cgcctgcagc aaggttccca cccacagcat acccggcatc caaagcacgt tgatatgggc
     1561 gttttccatt tttatcaaaa tgggtcaata cacctgtgct tgcgatgtaa tctgcatgag
     1621 attgggctat cttcatcaag attgcatccg atacataagg ttccagccca tttgaagcac
     1681 gcaaggtatt caccttctcg atcatctctt gggcagtttg gttgggagaa aaagcttcgg
     1741 gctgggcacc cgccttttcg gacggtttca gggcaagggt caagaaaaga agcgttgcaa
     1801 agatccaggg gtaggtcgat ttcattggga aaaattatag caggtgcctt cccaaaagtt
     1861 gagagtcaga ggtaaaatta tatataatgt ataatcttta ggaggcagca tatgccaggt
     1921 tttacgccgg gtcgtcggta tcaaccccca tttttgccgt tcaagcgcca gcctttcaaa
     1981 aaacgagcct ttgccgccat cgagcgcgca gtcaaaggtc ctttatttgg atgccgcatg
     2041 tgcggaaatt gcctcctgca agaaactgca ttaatctgcc ctatggaatg ccccaaaggg
     2101 ttgcggaacg gaccatgcgg cggctccacc cccgaacgct gctatgtgga tgaaagccgc
     2161 ccttgcattt ggtacaaaat ctacgaccgg tcgttcaaac ttgggcgcga agccttgttg
     2221 ctcgaagttt tgccccctat ggattggaat aaagccggca cagatgcatg gagcgacgtg
     2281 tttga
//
LOCUS       DAOREF010000536         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35901_length_2280_cov_4.103371, whole genome shotgun sequence.
ACCESSION   DAOREF010000536 DAOREF010000000
VERSION     DAOREF010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35901_length_2280_cov_4.103371"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..620
                     /locus_tag="PKI33_11015"
     CDS             9..620
                     /locus_tag="PKI33_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNM37589.1"
                     /translation="MDLFLHLDEYLQTIITTYGAWTYGILFVVIFIETGLVVMPFLPG
                     DSLLFAAGTFAALGSFNVWGLVGLLAVAAVLGDAVNYSIGHYLGDRAYNIKWIKKEYL
                     EQTHAFFEKHGGKAIFLARFVPIVRTFAPFVAGIGKMSYSYFATYNIVGGISWVTIFT
                     LLGFFFGNIPFVKKNFELVIIAIILISVVPMIYEWWKARRAAK"
     gene            674..1333
                     /locus_tag="PKI33_11020"
     CDS             674..1333
                     /locus_tag="PKI33_11020"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNM37590.1"
                     /translation="MNHIELSSKEESLLNQETLAEKMELVVEKLPADEVTLVEIMDIV
                     GADSLMLVTIFMSLIFLVPVSIPGVSTVFGTGILLIGITHLFARKLWLPKPIANRKLS
                     APKLKEAFKRAIVWLHRLEKVSRPHRLSKLTAEGGMTIFNNLSFILAALLLMAPFGFI
                     PFSNTLPALALIFFALGMMERDGGSVIFGHIANIATIIYFGFLIAGGGWSIGEFIKVL
                     G"
     gene            1378..1740
                     /locus_tag="PKI33_11025"
     CDS             1378..1740
                     /locus_tag="PKI33_11025"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNM37591.1"
                     /translation="MKYLAILFTLFILAIIILADKNALPPFIHAIYDFPNGDKAGHFI
                     LFGLLNFILTLTFLRALPNRTRSRVALSVGLILALAITLEEFSQRYFSSRTFDLIDLT
                     ASYIGVLVGGWIAFRIKK"
     gene            complement(1773..>2280)
                     /locus_tag="PKI33_11030"
     CDS             complement(1773..>2280)
                     /locus_tag="PKI33_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNM37592.1"
                     /translation="PDNFFWDDKLTTDVTETRDDIFIASLAETVQQLEKEYGKDPAKW
                     PRWGELHTATFRNGTLGESGVFLIEDLFNRGPFETNGGQSLVNATGWDLGTSFEVDWL
                     PSMRMIVDFNDLNASLTVHTTGQSGHAYHPHYDDMALFWADVSYYPMWWDQESVIDDA
                     EGHLVLKP"
BASE COUNT          524 a          533 c          501 g          722 t
ORIGIN      
        1 aaagtgcgat cgatctcttt ttacatctcg atgaatattt gcaaaccatt atcaccacct
       61 atggtgcctg gacctatggc attctttttg tagtgatctt tatcgaaacg ggcttggttg
      121 tgatgccctt cctgccagga gactccctgc tctttgctgc agggaccttt gctgcactcg
      181 gctctttcaa tgtgtggggc ttggtcgggt tgttggcagt tgctgcggtg ttgggcgatg
      241 cggtcaatta ctccatcggt cattatcttg gtgaccgtgc ctacaacatc aaatggatta
      301 agaaagagta tttggaacaa acccatgcct ttttcgagaa gcatggcggc aaggctattt
      361 tccttgcgcg ctttgttcct atcgtccgta catttgctcc tttcgtagct ggtattggca
      421 aaatgtctta ctcttatttt gccacctata acattgtggg cggcattagt tgggtgacga
      481 tctttacttt actcggcttt ttctttggca atatcccctt tgtgaaaaag aattttgagt
      541 tggtcattat tgccatcatt ttgatctcgg ttgtgccgat gatttacgaa tggtggaagg
      601 cacgtcgcgc cgcgaaataa actgtagtgt tctgaaatca ctttttactt tagaaaatac
      661 tgaggatatt aaattgaacc acattgaatt gtcttccaag gaagagtctt tgctaaatca
      721 agagactctt gccgaaaaaa tggagttggt tgttgaaaaa ctccccgcag atgaagtgac
      781 tttggtcgag atcatggata ttgtcggtgc tgatagcctc atgttagtaa ctatcttcat
      841 gtccttgatc tttttggtgc cggtttccat tcctggtgtt agtacggttt tcggaacggg
      901 tattctcctg atcggtatta ctcatctttt tgcaagaaag ctttggctgc ccaaacccat
      961 tgcgaatcgt aaactctcag cacccaaatt gaaagaggca ttcaaacgtg ctattgtttg
     1021 gttgcatcgt cttgaaaagg tcagccgtcc acatcgcttg agcaaactca cggcagaagg
     1081 tggcatgacc attttcaata atttgagctt cattcttgcc gctcttctgc tcatggcgcc
     1141 ttttgggttc attccgttta gcaacaccct tcctgccttg gcattgatct tttttgcact
     1201 tggcatgatg gaacgagatg gcggctccgt gatttttggg catatcgcca atattgccac
     1261 gatcatctac ttcggcttcc tcattgcagg cggcggatgg tctattggcg aattcattaa
     1321 agtgcttggt taactcttta taaggcaggc tgggttccaa gcccagcctg tttttttatg
     1381 aaatatctag ctattctttt tacactgttc attcttgcga tcatcattct tgcagataaa
     1441 aatgctttac caccatttat tcatgcaatt tatgattttc caaatggcga taaagccggg
     1501 cacttcatcc tttttggact cctcaacttt attctcactc ttacctttct ccgcgcgctt
     1561 cccaaccgga cccgtagccg ggttgctctt tcagtcggtt tgatactcgc cctcgccatc
     1621 accctcgaag aattctcgca acgttacttt tcctctcgta ccttcgattt gattgatctt
     1681 acagcaagtt atataggtgt tttagtaggc gggtggatcg ccttccgaat aaaaaagtag
     1741 gtcacgcctc aagcgtgacc tactttttta ttttagggct tcaacaccaa atgcccttcc
     1801 gcatcatcaa tgacggattc ttgatcccac cacatggggt agtaagagac atctgcccag
     1861 aatagcgcca tatcatcgta atgcggatga tatgcatgcc cggactgccc tgttgtatga
     1921 accgtaagag acgcgttgag atcattaaaa tccacgatca tacgcatgga gggcagccaa
     1981 tctacttcaa aggaagtgcc caagtcccag ccagttgcgt tgactaggct ttgaccgccg
     2041 ttggtctcga aaggtccgcg attgaagagg tcttcaatca agaagacacc agattcgcca
     2101 agtgtcccat ttctaaacgt ggcagtatgt aactcacccc agcgcggcca tttggcgggg
     2161 tctttcccgt attctttttc caattgctga acagtctcag ccaatgatgc aatgaaaata
     2221 tcatcgcgag tttcagtcac atcggttgta agtttatcgt cccagaagaa gttatcaggt
//
LOCUS       DAOREF010000537         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_35957_length_2278_cov_4.169141, whole genome shotgun sequence.
ACCESSION   DAOREF010000537 DAOREF010000000
VERSION     DAOREF010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35957_length_2278_cov_4.169141"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..988)
                     /locus_tag="PKI33_11035"
                     /pseudo
     CDS             complement(86..988)
                     /locus_tag="PKI33_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
     gene            complement(1061..1303)
                     /locus_tag="PKI33_11040"
                     /pseudo
     CDS             complement(1061..1303)
                     /locus_tag="PKI33_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            complement(1484..2206)
                     /locus_tag="PKI33_11045"
     CDS             complement(1484..2206)
                     /locus_tag="PKI33_11045"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNM37593.1"
                     /translation="MATKSTKTTKSTKTTKTTKPRASRAQSTPKVSKPAVRTGTWVAL
                     IAFIAVIATLLYMNRKAETEATATETPAAEETFIFSSESVVSSIEVKPAEGNAVKVER
                     NEEKTWVLSQPEKAEANQAASEAAATQIGALSIVTTIEGNKDPSIFGLDAPVYTITVT
                     FDDGKKGILEVGDTTPTSTGYYVRLDKDKVYVVAMDGIDALTSLTVAPPYLNTPTPVP
                     TATSTPLPTDTPATTTEATPTP"
BASE COUNT          502 a          561 c          622 g          593 t
ORIGIN      
        1 atggtcttca atcaaccacc ttaattgtag aatgaaaaaa acagcaacac acccaagggt
       61 gcattgctgt ttgcctttaa tcgaactact ctcccaagta atcgcgcaat ttcctgcgga
      121 tggaagggtg ccgaaggcgg cttaatgcct gagcttcgat ctgtcttacc cgctcgcggg
      181 tgacgcccat cttacggccc acttcttcga gggtgtaggc ctgaccgtcg agcaagccat
      241 agcgcagttg caaaatgcgc acttcacgcg ggggcaggct gttcagcact tcgccgaggt
      301 gttcgcgcag aagattgtac gtcgctgtat cgtcgggtgg tggagcctca tcgtcttcga
      361 tgaagtcacc cagcaccgaa tcttcttcgt catctgtcgg ggtttccagc gaaagcggac
      421 ggcgtgcgac ctggatcatg ttctccactt ttttgggagg cacgtccaag gcttcagcga
      481 gttcttccac gctcggctca cgcccgagac gttgagtcaa ctggtgctga acgcgcaaga
      541 gcttgttgat ttggtcgccc atgtggaccg gcacgcggat agtgcgtccc tgatcggcga
      601 tggcgcgggt cacggcttga cggatccacc acgtggcata agtcgagaat ttgtgaccac
      661 ggcgataatc gaatttcttg gtggcgcgga tgagaccgat gttgccctct tgaataaggt
      721 caaggaaggg tacaccacgt cccatatatt tcttggcaac ggagatcacg aggcgggagt
      781 tcgcggtaat gaggtgttca cgagctgccc agccgtcttc aataaaacgg cgaagttcga
      841 ggcggcgctt gggagaaaca gaaccatgcg caagttcttc gcgggcagag cgtccgcgtt
      901 cgatgcgctg ggcaagctga acttcttctg tagcggtcag caacggaacg cggctgacct
      961 ctttgaggta cagaccgatg gtgtcgtctg tatcaatatt ggctaaataa tcatccagcg
     1021 cgagatcggg ctcgggttcg gcttcaccgc tctcttctac agccaccaat tcgtcttcgg
     1081 tgggttcggg ggtcatgctt tcttccacga acgggatacc agcgcttaaa agcgcagaga
     1141 acgcttcttc aagctgttct acgtcttgtt ccgcttcggg aaagaagtgc aatatgtcgt
     1201 cgagagttac aaaggatttt tgccgcccca gttcgatcaa acgcgctata gcagaatact
     1261 cttcttcatc atcttcgcta attagtatct tatctacatc cattcacatc atccaacttt
     1321 caacaaatat ttgtcttaca tttcagaata cacgtttttg aaccgaaaag caatagtgtt
     1381 cggcttatca gatgccttgc aatgattttg tattactttt tggaggaatc gggtcgggga
     1441 atctgcttca attttcaaac agattccccg acgccgtaaa aacttagggg gtaggagtag
     1501 cttcggttgt tgtagcaggc gtatcggtcg gaagaggcgt ggatgtcgcc gttggaaccg
     1561 gtgtgggggt attcaaatat ggcggggcga cggtcaggct ggtgagcgcg tcaattccgt
     1621 ccatagcgac aacatacacc ttatctttat ccaaacgcac gtagtaacca gtggaggttg
     1681 gggtggtatc gcccacttcg aggatgcctt ttttgccatc atcaaaggtg actgtgatgg
     1741 tgtaaaccgg ggcatccaaa ccgaagatgg aggggtcttt attgccttca atggtggtga
     1801 caatgctcag cgcaccaatt tgagtggcag ccgcctcaga cgcagcctga tttgcttctg
     1861 ctttttcagg ctgagataac acccaggttt tctcttcgtt tcgttcaacc ttaactgcgt
     1921 taccttcagc aggcttgact tcaatactgc tgacaacgct ctcgcttgag aagatgaaag
     1981 tctcttcagc cgccggggtt tctgtagcgg tcgcttctgt ttctgccttg cggttcatgt
     2041 acagtaaagt ggcaatcaca gcgatgaaag cgatcagcgc aacccatgtt ccggtgcgga
     2101 cggcgggttt ggagaccttt ggggtgcttt gcgcacgaga cgcgcgtggt ttggtggtct
     2161 tggttgtttt tgtagatttt gttgttttgg tcgatttcgt agccatgaat taacctcgtt
     2221 tacgacgggc aagccatgca gacacgccaa agaaaacaac cataccgggg atgataat
//
LOCUS       DAOREF010000538         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36108_length_2272_cov_5.077582, whole genome shotgun sequence.
ACCESSION   DAOREF010000538 DAOREF010000000
VERSION     DAOREF010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36108_length_2272_cov_5.077582"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1070)
                     /locus_tag="PKI33_11050"
     CDS             complement(144..1070)
                     /locus_tag="PKI33_11050"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNM37594.1"
                     /translation="MQHYRSLEEVSLKNSYVTVGVFDGVHRGHREIIQKLVQDAHANH
                     APAVVLTFSPHPATVLTGREIHCLTTPDERADLLGSLGVDAVITQRFTRDLSTVPAHE
                     YMSSLKTHLGLSHLLIGYDFALGKGREGNAPRLAEIGTELGYTVEVVPALGDESGVIS
                     STEIRKLVSIGNMPEASKLLGYPYRMSGEVIHGAGRGKQINFPTANVDYAPQKASPPN
                     GIYACWAKLGDERFMAATNIGFNPTFTPERKTQSLEAYLLDFDRDIYGDVLTLEFVAR
                     LREEIRYTNVEALIEQIHDDVLQTRKLLSDLK"
     gene            complement(1117..2046)
                     /gene="truB"
                     /locus_tag="PKI33_11055"
     CDS             complement(1117..2046)
                     /gene="truB"
                     /locus_tag="PKI33_11055"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNM37595.1"
                     /translation="MNSQDVKNAISGALVIDKPVGMTSHDVVQAIRNGTGLRRAGHTG
                     TLDPRASGVLVILVGPAVRLSEYVSASDKRYQAIIRLGGTTDTYDAEGKFTPTKDPSH
                     ITEAEFEEALKTFVGEIEQTPPPYSAVKVQGRKAYEMARKGEEVELEPRTITVHHLEV
                     LEWTPPEVVIDVHCSSGTYVRSLANDLGKKLGCGAYLVGLRRTKSGKFTLRDAVPLRK
                     LQEAFNAGNWYQYLIPAAEALGDWPAIELNPDEVEAVRHGHRVVAKASDIVHEKVRGV
                     STQGELVALMVQADNVETGNKEWQPKKVFFTSD"
BASE COUNT          546 a          548 c          550 g          628 t
ORIGIN      
        1 ccgccgccgg gaaaagcaaa ataatgccgc ccttttttgt agcgttcaat cagtgccagc
       61 tttccttcat gaatcaaaat aattcctgaa cgtttaggca tatcactcct ttgaaacgca
      121 aaaaatatcg ggcaattgcc cgattatttc aaatcactca atagcttacg ggtctgcagc
      181 acatcatcat gaatctgttc gatcagcgct tctacgtttg tgtaacgtat ttcttcccgt
      241 agccgggcaa caaattccag agtcaatacg tcaccataaa tatcacggtc aaaatcgagt
      301 aaataggctt ccaagctctg agtttttcgc tcaggggtaa atgtagggtt gaagccaata
      361 tttgttgctg ccataaaacg ttcatcgccc agttttgccc aacaagcata aataccattg
      421 ggcggagacg ctttctgggg agcataatcg acattcgcag tagggaagtt gatttgcttt
      481 ccgcgcccag ccccgtgaat gacttctccg ctcatgcggt agggataccc aagcagtttg
      541 gatgcttcgg gcatattgcc gatggaaacc agcttgcgaa tctcggtgga tgagatcacg
      601 ccgctttcgt cgcccaaagc tggtacaact tcgacggtgt atcctaactc cgtgccaatc
      661 tcggcgagac gtggtgcatt gccttcacgt cctttcccga gggcaaaatc ataaccaatt
      721 agcagatggc taagcccaag gtgagttttc agactggaca tatattcatg ggcagggaca
      781 gttgaaaggt cccgtgtgaa gcgttgggtg ataaccgcat ccacacccaa ggaaccgagc
      841 aaatccgctc gttcatcagg tgttgttagg caatggattt ctctgcctgt gagtactgtt
      901 gccgggtgcg gcgaaaaggt taacaccaca gctggggcat ggttggcatg agcatcttgt
      961 accagttttt gaatgatctc gcgatgaccc cggtggacac cgtcgaaaac acccacggtt
     1021 acatacgaat ttttgagaga aacttcttca agagagcgat aatgttgcat atgaaaaaag
     1081 ccgcctttga cggcggcttt attttatcat tccgtgttaa tcagacgtga agaacacttt
     1141 tttaggttgc cattctttgt tgccggtttc gacattatct gcctgaacca tgagtgcaac
     1201 caattcgcct tgggtgctga cgccgcgcac tttttcgtgt acgatgtctg aggcttttgc
     1261 aaccacgcga tgaccatgac gtacggcttc cacttcgtcg gggttgagct caatagcggg
     1321 ccaatcgccg agtgcttcag ccgcagggat gaggtattga taccaattgc ctgcattgaa
     1381 ggcttcttgc agtttgcgca gcggaacggc atcgcgcagg gtgaactttc cgctcttggt
     1441 gcggcgtaag ccaacgagat aagcgccaca gcctaatttt ttaccgaggt catttgccag
     1501 cgaacggaca tacgttccgg acgagcaatg aacatcgatc accacttcgg ggggagtcca
     1561 ttcgagcact tcgaggtggt gaacggtaat ggtgcgaggt tcgagttcta cttcttcgcc
     1621 tttgcgcgcc atttcgtaag ctttgcgtcc ctgtacctta acggcagaat agggaggtgg
     1681 ggtttgctcg atttcaccca caaatgtttt caatgcttct tcaaattccg cttcagtgat
     1741 atgagaaggg tctttggtgg gggtgaactt tccttccgca tcgtaggtat cggtcgtgcc
     1801 gcccaagcgg atgatggctt gatagcgttt atcggatgca gaaacatatt cacttaaacg
     1861 aacggcggga cccactaata taacgagtac gccagaagct ctggggtcta atgtaccagt
     1921 atgccctgcg cggcgaagtc ctgtgccatt gcgaatggct tgtactacat cgtgagaggt
     1981 catacccacg ggtttatcga taacgagcgc gcctgatatg gcgttcttta catcttgact
     2041 attcatctta caatcctcct acttcattgt cctttttatt atgacatatt cgttattttt
     2101 ttcaatgcat taatagtgct gtagtactaa actatactta caaattcaat aatttttttg
     2161 taacgtttaa ggcgtctttt ttaatgtcag ctaaacttcc ctcaatatct gctcctgcag
     2221 cggctgcatg accgccaccg ttgaagtgtt tggcgatcgg cgaaacgtca at
//
LOCUS       DAOREF010000539         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36167_length_2270_cov_4.567043, whole genome shotgun sequence.
ACCESSION   DAOREF010000539 DAOREF010000000
VERSION     DAOREF010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36167_length_2270_cov_4.567043"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1254)
                     /locus_tag="PKI33_11060"
     CDS             complement(187..1254)
                     /locus_tag="PKI33_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNM37596.1"
                     /translation="MEFKINNHTIGLNHPTYFIADIAANHDGDLDRAKLLIRLAKEAG
                     ADAAKFQHFQAPKIVSEYGFTHMNSKVSHQANWKRSVTEVYAAASVSQSWTPILKEEC
                     DKVGIDFFTSPYDYDSIEHVNPYIPAFKAGSGEIDWIEALVHMASKGKPMIIACGAAD
                     IADVQRAVQAILKVNKQLCLMQCNTNYTANPNNYDHLHLNTLKTFAAMYPDVILGLSD
                     HTHSVAPVLGAITLGARMIERHFTDSNDREGPDHKFAMNPANWAKMVEETRLLERSLG
                     SSDKFIAANEQETKVVQRRCLRAARDIKAGETFTRDMIDVLRPATPGAVKPHEIENVI
                     GTKALADMPKGKELRWTDLGA"
     gene            complement(1287..1742)
                     /locus_tag="PKI33_11065"
     CDS             complement(1287..1742)
                     /locus_tag="PKI33_11065"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNM37597.1"
                     /translation="MRFPNGFVIDKYHLLDFEREAVVALMENADGEVIFALIPRYPSN
                     TVEWELPAGGMEVGEEILEAAKRELLEETGYSSTDHKLIYTYYPMNGNANQVFHIVFC
                     KAGERVQDFDLNEVADVRWFKREEIKQLLKDQKIKDGFSLTALLLWLQN"
     gene            complement(2073..>2270)
                     /locus_tag="PKI33_11070"
     CDS             complement(2073..>2270)
                     /locus_tag="PKI33_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37598.1"
                     /translation="ESDITPKSVNFSGLIAKRSNNLWLSTRKLSADLGEDLPNFSTGL
                     DEFYTQYEQGYPQKIRGYQQP"
BASE COUNT          582 a          511 c          556 g          621 t
ORIGIN      
        1 ttctcgggta ccgagcgact ttctacttgc cgccggttgc cttcaagctg cacaaagtga
       61 acatcatgcc cgccatcaga aatggcttta agaaaacgat aatcgtgtgt ggaatagcca
      121 agggagaaat aaagaatctt catatgcaaa cgtaggggcg gggtaatccc gcccctacaa
      181 atttaattac gcacctaaat ctgtccaacg caattctttg cctttgggca tatctgccaa
      241 cgctttggtg ccgatgacat tctcgatctc atgcggtttg accgcgcccg gtgtggcggg
      301 gcgcagaaca tcgatcatat cgcgggtgaa ggtctcaccg gctttgatat cgcgggctgc
      361 tcgcagacag cgacgttgaa cgactttagt ctcttgctca ttggcagcaa tgaatttatc
      421 ggagctaccc agcgaacgtt cgagcaaacg agtctcttcg accatcttcg cccaattggc
      481 aggattcatc gcaaacttgt gatcaggtcc ttcgcggtcg ttgctgtcag tgaagtgacg
      541 ttcaatcatg cgtgcgccga gagtgatcgc gccgagaacc ggcgcaacag aatgcgtatg
      601 gtcagacaaa ccaagaatca catcggggta catggcggcg aaggttttga gcgtgttcaa
      661 atgcaggtga tcgtagttat tggggttggc ggtgtagttt gtgttgcatt gcatgagaca
      721 caactgtttg ttgactttga gaatggcttg gacggcacgc tgtacgtcag cgatatcagc
      781 ggcgccgcaa gcaatgatca ttggcttacc tttggacgcc atgtggacaa gcgcttcgat
      841 ccagtcaatt tcgccagagc ccgctttaaa agctggaatg tatggattga catgctcaat
      901 cgagtcataa tcgtacggtg atgtgaagaa gtcgatacca actttatcgc attcttcttt
      961 gagaatgggc gtccaacttt gagaaacaga agccgccgca tagacttcgg taacggaacg
     1021 cttccaattt gcctggtggc tgaccttgga attcatatga gtgaaaccgt actctgaaac
     1081 gatcttcggc gcttgaaaat gctggaactt ggcagcatcg gctccggctt ctttggcgag
     1141 gcggatgagt agcttggcac gatccaaatc accgtcgtga tttgctgcga tatcagcgat
     1201 gaagtaggtc gggtgattca atccgatagt atggttattg attttgaatt ccatgtcgtc
     1261 tctcctttat ttcgatgaaa gattatttag ttttgcagcc ataaaagcaa tgcagttagt
     1321 gaaaagccat ccttgatctt ttggtctttc aacaactgtt tgatctcttc ccgcttaaac
     1381 cagcgaacat cagccacttc attcaagtcg aaatcttgca cgcgttcacc ggctttgcaa
     1441 aatacaatat gaaagacctg attggcattc ccgttcatgg ggtaataggt gtagatcaat
     1501 ttgtgatcgg ttgagctata ccctgtttct tccaataact cgcgtttggc ggcttcgaga
     1561 atctcttcac caacttccat gccgcctgcg gggagttccc attcaaccgt attacttggg
     1621 tagcgaggga tcagggcaaa aattacttcc ccgtccgcat tttccataag ggcaaccaca
     1681 gcttcacgtt cgaaatccaa taaatggtat ttatcgatca caaaaccatt cggaaatctc
     1741 acagtgtcct tgtataaatt aacccatcta ctttggtaca cgacctctct tgccaatctt
     1801 tctggcttca tggtgaaaga aaatctcctc gtaactgttt tatcaacaag ggctgtgggt
     1861 aaaacagtgg ataaacacgt cacagagctg ggttattgtg tgtaaacgta ctccagcctg
     1921 tggataagtg tgtggattct gtggataact cggttcaaaa cctcgggatt ttggcaagat
     1981 ctgcctgatt cctgtggaaa aaccccaatt taagtgggga aatctgtgga taagtggcag
     2041 aaaaagatgc caaaaatcgg ctcaaactgt ggctaaggtt gttgataacc acgaattttc
     2101 tgtggataac cttgttcgta ttgtgtgtaa aactcgtcca aacctgtgga aaagttgggg
     2161 agatcctcac ccaaatctgc ggataacttg cgggtagaaa gccataaatt gttggaacgt
     2221 ttggcaatta atccggaaaa gttgacagat tttggggtaa tgtcactttc
//
LOCUS       DAOREF010000540         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36176_length_2270_cov_3.233409, whole genome shotgun sequence.
ACCESSION   DAOREF010000540 DAOREF010000000
VERSION     DAOREF010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36176_length_2270_cov_3.233409"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PKI33_11075"
     CDS             complement(<1..1017)
                     /locus_tag="PKI33_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNM37599.1"
                     /translation="MSLIQEKTDQAVQILKEQDIDLWLTFVRETSGVRDPVLSFIIGE
                     ADLTWPSALIMTKHGDKIAIVGNLETEALQRLDVFTQIIGYDTGVSGVLRETISKLNP
                     NQIAVNMSRNNVHADGLTHAMYEFLVEYLSGTPYPERIISAEPIINALRGRKTKAELA
                     RIQRAVEITDEIYKKTFAFIKVGMTEIQIADYMQALMKEYGVGYAWPRENNPAVNSGP
                     DKVVGHNGPTDIKVEPGHIIHFDFGVKHEGYCSDIQRIAYVLREGETEAPVEVQRGFI
                     TVRTAIEKSREAMKAGVIGNDIDTISREIIVDSGYPEYMYALGHQLGRVAHDGGALLG
                     PRWEK"
     gene            complement(1033..>2270)
                     /locus_tag="PKI33_11080"
     CDS             complement(1033..>2270)
                     /locus_tag="PKI33_11080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37600.1"
                     /translation="LKHNVTAGLWDGSLFGVALGFASFGTILPLFVATMTNSATLIGL
                     VPAIHSAGWLFPQLFTASHVSRLRQLKRTVLLNTIHERVPFLGFAAVALLLPVVGVNA
                     GLLLTFLLLIWQGLGGGFTANAWTSMVSKIIPPESRGTFFGMQAGLANLFISGSAIAA
                     GYLLNALASPWDFAVCFFIASIFFALSWFALAATREPEDTEKFIPEEKSHFWDDAKKV
                     LSRDTNFNWFLIARILSQFATMGFSFYIIYALREFNMTEVVAGYLTATLTISQTIANI
                     GMGWIGDRVGHRVMLIIGAFSAFLSALLAWNAASIAWFYPIFILTGLANVSMWTISMT
                     MTVDFGNETDRPIYIGLSQTLTAPATIIAPILGGWIADSAGFVPTFTISTILSLVMVG
                     ILIFLVKDPRKSKLVNLQS"
BASE COUNT          582 a          535 c          612 g          541 t
ORIGIN      
        1 tttttcccaa cgcggaccca acaatgcgcc accatcatgt gctacacgcc caagttgatg
       61 acccagcgca tacatatatt cggggtatcc cgagtccacg atgatttcgc gtgagatagt
      121 atcgatgtca ttcccaatca caccagcctt catcgcctca cgcgatttct ctatcgccgt
      181 gcggacggtg ataaacccgc gctgaacttc tactggcgct tcagtttcgc cttcgcgcaa
      241 cacataagca atgcgttgaa tgtcggaaca atagccttcg tgcttcacgc caaagtcaaa
      301 gtggatgata tgacctggct cgaccttgat gtcagtcggt ccgttatgcc caaccacttt
      361 atcgggtcct gaattgaccg ccgggttatt ctcgcggggc caggcatagc caacaccata
      421 ttctttcatc aaggcttgca tgtaatctgc gatctgaatt tcggtcatgc cgactttgat
      481 aaaagcaaac gtctttttgt aaatttcgtc tgtgatttca acggctcttt gaatgcgggc
      541 aagttcggct ttggtcttgc gtccacgaag ggcgttgatg atcggctccg cgctgataat
      601 gcgttcgggg tatggtgtgc ctgagagata ttccaccaga aattcataca tggcatgtgt
      661 caacccatcg gcatggacgt tgtttctgga catattgacc gcgatttgat tcgggttgag
      721 ttttgaaatg gtctcacgca gcacaccgct gacgcccgta tcgtacccaa tgatctgagt
      781 gaacacgtcc aagcgctgca atgcttcagt ttcgaggttg ccgacaatcg caattttgtc
      841 tccgtgtttg gtcataatga gcgcggaggg ccaagtcaga tcagcttcac cgatgatgaa
      901 actcaacacc gggtctcgga ctccgcttgt ctcgcggacg aaggtgagcc agaggtcaat
      961 atcttgctct ttgagaattt gaacggcttg gtcggtcttt tcttgtatga gggacataga
     1021 tgctccttgg atttaagatt gtaaatttac gagtttcgac ttacgcgggt ctttgacgag
     1081 gaagatcaaa ataccaacca tcaccaatga aaggatggtt gagatcgtaa atgtaggtac
     1141 aaacccagcg ctgtctgcga tccagccgcc gaggatgggg gcaataatgg ttgctggggc
     1201 ggtgagtgtt tgtgataagc caatgtaaat tgggcggtcc gtctcattgc cgaagtctac
     1261 ggtcatggtc atgctgatgg tccacatgga gacatttgcc aagccagtga gaatgaagat
     1321 ggggtaaaac catgcaattg aagcggcatt ccacgcaagt aaagcgctta ggaatgcaga
     1381 gaatgcgccg atgataagca tgacgcgatg cccaacccgg tcaccgatcc agcccatgcc
     1441 aatgttggca atcgtttgtg aaatggtaag ggttgcggtg agatatcccg ccactacttc
     1501 ggtcatattg aattcgcgta gggcgtagat aatgtagaac gagaatccca tggtcgcaaa
     1561 ttgggagagg atgcgtgcga tcaagaacca gttgaagttg gtatcgcggc tcaatacctt
     1621 tttggcatcg tcccagaaat gagatttttc ttcggggata aacttttcgg tatcttcagg
     1681 ttcgcgggta gcggctaggg cgaaccagga taaggcgaag aaaatgctgg caatgaagaa
     1741 acagaccgca aagtcccagg gagaggcgag ggcgttaagc aggtatcccg ccgcgatggc
     1801 agatccgcta atgaagagat tcgccagccc ggcttgcatc ccaaagaaag tgccgcgtga
     1861 ttcgggcgga atgatcttgg agaccattga ggtccatgcg ttagcagtaa aaccaccacc
     1921 aagcccttgc catatcaaaa gcaaaaacgt aagcagtaaa ccggcattca cgccaacgac
     1981 ggggagtaag agtgctaccg ccgcaaaccc taaaaaggga acacgttcat gaatggtatt
     2041 tagtaacacg gtgcgtttta gttggcgtaa gcgggagacg tgactggcag taaagagttg
     2101 tggaaaaagc cagcccgccg aatggatggc aggcaccaag ccaattaagg ttgcagaatt
     2161 ggtcatggtg gcgacgaaga gcggcaggat ggttccgaag gaggcaaagc ctaatgccac
     2221 accgaataga cttccatccc atagcccggc agtaacattg tgtttaaggt
//
LOCUS       DAOREF010000541         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36301_length_2266_cov_3.140660, whole genome shotgun sequence.
ACCESSION   DAOREF010000541 DAOREF010000000
VERSION     DAOREF010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36301_length_2266_cov_3.140660"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PKI33_11085"
     CDS             complement(<1..1292)
                     /locus_tag="PKI33_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37601.1"
                     /translation="MKTIVRSLALLSIASLLLASCTLFGSSTEPAANALSEVQATQSL
                     TDLVTIELTTQYDTAAVYNTVGQTVRFKYLVKMVKNDLPDGTPANVTITGITATCPAL
                     NTINNLNDRFDPGEVLECTGDYLITQADLDKGSIANVVTANAYSVNSNTVTTTVPTVS
                     PKALTLTKTASTTTYGAANQQITFTYTVKNSGTVQLGPAQFTIADTGISNNQPFNCGN
                     ADATIAPGATLNCNATYTVTAADMNATTISHSATVSGGGVTSQPAALSLTKGSAPAAA
                     SGTTVQHTVREGEWLWQIARCYSADPNQTVSANPQVDPKNLKIGTTVTVPNVGTVGNR
                     AVHAPPELCVQLHTVQSGDTWTSIADKYGADPGLTQLSNSNNMIVGKQVKVPLYTKGL
                     NIPLSNAVATVSPTTTGMSLTVTPNTTIYTQVDQIITF"
     gene            complement(1426..1911)
                     /locus_tag="PKI33_11090"
     CDS             complement(1426..1911)
                     /locus_tag="PKI33_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37602.1"
                     /translation="MANGQVKKIATPKAAGYFIVYKVQPTDKSLKQIAREQLADEKLW
                     EKIVLDRETANPKGFFWDEIPANSFDPRWTLLLPPSEFAAFTISVNTPIHRAPNGEFL
                     AGANITEKYFYARKTVVVSGQKVWADVTKTNPARFGNKPYWILVKDGTSKTNPPISGP
                     S"
     gene            complement(1977..>2266)
                     /locus_tag="PKI33_11095"
     CDS             complement(1977..>2266)
                     /locus_tag="PKI33_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37603.1"
                     /translation="IATPIWDKAEELKAEQYQHTDFYEPGKKFQKYAIANGRKGLPPE
                     AVSKIVFKALTLSHPRVRYAVDPNPFSNWVMSILPKRTIDKILAKGLGFNK"
BASE COUNT          539 a          469 c          594 g          664 t
ORIGIN      
        1 ttaaaggtga tgatctggtc tacctgtgtg taaatagtcg tattcggggt gaccgttagc
       61 gacattcctg tggttgttgg agaaaccgtg gcaaccgcat tggaaagtgg aatatttaaa
      121 cccttggtat aaagtggaac tttaacctgc ttgccgacga tcatattgtt gctgttagag
      181 agttgtgtga gcccggggtc tgcaccatat ttatcggcga tggacgtcca cgtatcgcca
      241 gattgaacag tatggagctg aacacaaagc tctggcggtg catggacggc gcgattcccg
      301 acagtgccaa cattgggaac agtgactgta gtgccgatct tcaagttttt cgggtccact
      361 tgcgggttcg cgctaacggt ctgattggga tctgccgaat aacagcgggc gatttgccat
      421 agccattctc cctcgcggac ggtgtgttgc acggttgttc ccgaagcagc agcgggcgcg
      481 gaacctttgg ttagtgaaag agccgcgggt tgtgaggtca caccgccacc tgaaacggtt
      541 gcagagtgag aaatagtcgt cgcgttcata tctgcagctg taacggtgta ggtggcgttg
      601 cagttcaagg tcgcaccggg ggcaatggtt gcatctgcat tgccgcagtt gaacggctga
      661 ttattgctaa tacccgtatc ggcaatggtg aattgtgccg gtcctaattg aacagttcca
      721 ctattcttaa cggtataggt aaatgtaatt tgctgatttg ctgcaccata agttgtggtg
      781 cttgccgttt tcgtaagcgt gagtgctttg ggcgaaacgg ttggcactgt ggttgtcact
      841 gtgtttgaat taactgaata cgcattggca gtgactacat ttgcgattga acctttatcg
      901 aggtctgctt gggtaataag ataatctcca gtgcattcaa gcacttcgcc agggtcgaag
      961 cggtcattga gattattgat agtgttgagc gccgggcaag tggctgtaat gcctgtgatc
     1021 gtaacatttg ccggagtgcc gtcaggcagg tcatttttga ccattttgac gagatactta
     1081 aagcgaacag tttgcccaac ggtattgtag actgctgctg tatcgtattg agttgtaagc
     1141 tcaatcgtga ctaaatctgt aaggctttga gttgcctgaa cttcagacaa tgcatttgcg
     1201 gcgggttctg ttgagctgcc aaagagcgtg caagatgcca gcagtaaact ggcaatgctc
     1261 aacagggcaa gcgagcggac gatggttttc atgaccactt cctttcgttt tgtaaaattg
     1321 tacgaccact tttattatat cctctataca tctggattaa gaatcatccc gcagggttct
     1381 taagtctact ctgcgggatg ataatgcgag gattaaagca acaaattaac tgggaccgga
     1441 tataggtgga tttgttttgg atgtaccgtc cttaacaagg atccagtaag gcttgttccc
     1501 gaatcgtgcg ggatttgtct tggttacatc tgcccaaact ttttgcccac tgacaacgac
     1561 tgtcttgcgc gcataaaaat atttctcagt gatattcgcg ccagccagaa attctccatt
     1621 aggcgctcta tgaataggcg tgttgacgga gatggtaaac gctgcaaatt cagaaggcgg
     1681 cagcaacaaa gtccaacgtg ggtcgaaaga gttggcaggg atctcatccc agaaaaagcc
     1741 tttgggattc gcggtttcgc ggtctagaac gatcttctcc cagagctttt catcggcaag
     1801 ctgttcacga gcaatttgtt tcaagctttt gtctgtaggc tgaactttat atacaataaa
     1861 atacccggct gcttttggag ttgctatttt cttaacttgt ccgtttgcca tttttctctc
     1921 ctttgttttt gaaccacaac tatagaatac ccgaatagaa gtggatgtca atagctttat
     1981 ttgttgaagc caagtccctt tgcgaggatt ttatcaattg tacgtttggg taagattgac
     2041 atgacccaat ttgaaaatgg attggggtct actgcatagc gcacgcgagg atgggaaaga
     2101 gtcaacgctt tgaatacgat cttgctcaca gcttcaggtg gaagaccttt gcgtccattg
     2161 gcgatggcgt atttttgaaa cttcttgccc ggctcataaa aatcagtatg ttgatattgc
     2221 tcggctttta gttcttcggc tttatcccag attggtgtag caattg
//
LOCUS       DAOREF010000542         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36353_length_2264_cov_4.322318, whole genome shotgun sequence.
ACCESSION   DAOREF010000542 DAOREF010000000
VERSION     DAOREF010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36353_length_2264_cov_4.322318"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1838)
                     /gene="ade"
                     /locus_tag="PKI33_11100"
     CDS             complement(48..1838)
                     /gene="ade"
                     /locus_tag="PKI33_11100"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNM37604.1"
                     /translation="MSSSTKLATALVDVAMGRANADLVVKNGKWVSVQSGEIVPNIDI
                     AVVQGHIAFVGRDASHTIGKKTKVIDAKGHYLVPGLLDAHMHVESGMVTVTEFVRAVA
                     VRGTTGMFIDPHEIANVFGLKGVKLMVDEAQKQPIHVWVQMPSCVPSAPGLETPGSSI
                     GPKEVAEAMTWKGIIGLGEMMNFPGVFMSDKKMLDEMSATHAAGKTIGGHYASPDLGL
                     PFHGYVAGGAEDDHEGTRMDDAIQRVRQGMKAMLRYGSAWHDVAAQVKAITEKKLDSR
                     RFLLCTDDSHAETLTTEGHMDRVLRHAISEGLNPMTAIQMMTINTAEHFGLTKEMGMI
                     APGRWADILLVEDLMNFSADLVIAKGQVIAEKGEWQVKLPVVKYPKWVTNSVHLKRKL
                     TATDFVLRTRAAHGSEVEAHVIGVIENQAPTRHLKMKVKAENGEVKADIKQDLAKIAL
                     VERHRATGKVTVGLVSGFEFTRNCAIGSTVAHDSHHMIVVGNDDESMAVAANELAKCG
                     GGQIVVMDGKVIGQVEFPIAGLMSNERADVVAKKAATVLEGFKMCGSELNNPNMQLSL
                     MALVVIPELRISDKGLVDVTQFDFIPVLED"
     gene            complement(1866..>2264)
                     /locus_tag="PKI33_11105"
     CDS             complement(1866..>2264)
                     /locus_tag="PKI33_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNM37605.1"
                     /translation="HLLAEVRNAMLVSRVKEGLTGYSLSNDPNRKLMTAREALHLGTR
                     GGAAVLGRKDIGSLEPGKCADFFAVNLNKLGYAGMHDPVSAIVFGQSFNVDYTVVGGK
                     FVVKEGQLVTVDQNKLIEKHNKAAKRLLAG"
BASE COUNT          506 a          649 c          544 g          565 t
ORIGIN      
        1 gtttgtaatc gttgaaaagg aaaattagcc ataggttgtt ctctctctta gtcttccaac
       61 acaggaatga aatcgaattg ggtcacatcc accagaccct tatcagaaat tctcaattca
      121 gggataacca ccaatgccat caaactcaat tgcatattgg ggttattcaa ttcactgccg
      181 cacatcttga aaccttcaag caccgttgcg gctttctttg ccaccacatc ggcacgttca
      241 ttcgacatca aacctgcaat tgggaactca acctgcccaa tcaccttgcc atccatcacc
      301 acgatctgac cgccgccaca cttcgccagt tcattcgcgg caacagccat gctttcatca
      361 tcgttgccca ccacgatcat atgatggctg tcatgcgcca cggttgagcc aatggcgcaa
      421 ttccgcgtaa attcaaatcc gctcaccaag ccaaccgtca ctttgcctgt ggcacgatga
      481 cgttccacca gtgcgatctt cgctaaatct tgtttgatat cggctttgac ttcgccattc
      541 tcagctttga ccttcatctt tagatgacgt gtcggtgctt gattttcgat gacgccgatc
      601 acatgcgcct ctacttcgga cccgtgagcg gctcttgtcc gaaggacaaa atctgtcgct
      661 gtaagcttgc gctttagatg aacggagttg gtgacccact tgggatattt caccaccggc
      721 aacttgacct gccactcgcc cttctcggca atgacttgcc ctttggcaat caccagatcg
      781 gcgctgaaat tcattaggtc ttccaccagc aaaatatctg cccatctgcc cggagcgatc
      841 atgcccatct ctttggtgag accgaaatgt tcagcggtgt tgatggtcat catctggatc
      901 gccgtcatcg ggttcaaacc ttcgctgatg gcgtgccgca aaacgcggtc catgtgacct
      961 tcggtggtta acgtctcggc atgtgagtca tcggtgcaga gcaagaaacg ccgtgaatcc
     1021 agtttctttt cggtaatggc tttgacctgc gccgccacat catgccacgc ggacccatac
     1081 cgcagcatgg ccttcatccc ctggcggacg cgctgaatcg catcgtccat gcgggtgcct
     1141 tcgtggtcat cttcggcgcc gcctgctaca taaccgtgga agggcaaacc gagatcaggc
     1201 gaagcgtaat gcccgccgat ggttttgcct gccgcatggg ttgcggacat ttcatccagc
     1261 atcttcttat cgctcatgaa gacgccgggg aagttcatca tttcgcccaa gccgatgatg
     1321 cctttccagg tcatcgcttc ggcaacttct ttcggtccaa tggaagagcc gggggtttct
     1381 aatcccggag cggacggaac acaagagggc atctgcaccc atacatgaat gggctgcttc
     1441 tgggcttcat ccaccatcaa cttcacgcct ttgagaccaa agacattcgc gatctcatgc
     1501 gggtcgatga acatgccggt ggtgccgcgc actgctacgg cacgcacgaa ctctgtcact
     1561 gtgaccatgc cggattcaac gtgcatgtgt gcatcaagca gaccgggcac gagataatgt
     1621 cccttggcat caatgacctt ggtctttttg ccgatggtat ggctggcatc gcgccccaca
     1681 aaggcaatat gcccttgcac caccgcaata tcgatattcg gaacgatctc acccgattgc
     1741 acgctcaccc acttgccgtt cttcacgaca aggtcggcgt tggcgcgacc catggcaaca
     1801 tcaacaagag ccgtggcgag ttttgtggaa gatgacataa gatctccgtt ctgggttgaa
     1861 aaaggttaac ctgccaacag tcgtttggca gccttattat gtttttcgat gagcttgttt
     1921 tggtctaccg tcacgagttg accttctttg acgacaaact taccgcccac cacggtgtaa
     1981 tcgacgttga aggattgtcc gaagacaatg gcggagacgg ggtcatgcat gccagcatag
     2041 ccaagtttgt tcaagttcac ggcgaagaag tcggcgcatt tgccgggttc caaactgccg
     2101 atatctttac gtcctaaaac cgctgcaccg ccacgagtgc ccaaatgcaa ggcttcgcgg
     2161 gctgtcatca attttcggtt gggatcgttc gagagggaat agcccgtcag tccttctttg
     2221 actcgcgaga cgagcatggc atttcgcact tctgcaagga gatg
//
LOCUS       DAOREF010000543         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36447_length_2261_cov_4.708069, whole genome shotgun sequence.
ACCESSION   DAOREF010000543 DAOREF010000000
VERSION     DAOREF010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36447_length_2261_cov_4.708069"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PKI33_11110"
     CDS             <1..1087
                     /locus_tag="PKI33_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNM37606.1"
                     /translation="IELSTVMETEINLPYITADASGPKHLQLKLSRSKFEQMTEDLLQ
                     RCRKPFEAALKDAGMDASKLDEVVLVGGSSRMPMVQDLVRKLTNGKDPNKGVNPDEVV
                     AIGAAIQGGVLGGDVKDILLLDVTPLSLGVETMGNVMTVMIERNTTIPVRKTEVYSTA
                     ADNQTAVDIHVLQGERPMAGDNMSLGRFRLDGLPPAPRGVPQVEVTFDIDANGILHVT
                     AKDKATGKEQKVTITASTNLNKSDIERMVQESKQNEAADKKRREAIQVKNDADNMTYQ
                     IEKALRDLGDKVPSAEKASIETKVNELKQAAQGDDIEHIKKLTEELQNTFHALSQQMY
                     AQGQGGQPQPEGGPAPDGNVVDGEVRE"
     gene            1150..>2261
                     /locus_tag="PKI33_11115"
     CDS             1150..>2261
                     /locus_tag="PKI33_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNM37607.1"
                     /translation="MKKSFNRLWFIPIFIVLSIGVYYLPPVHSRLAWRIELVRTQIKY
                     WLNPPDEAVFQPSGETSPLTVETAVATTRAEFLLTLTPTSTPTPELVATIEPTITSTP
                     LPTTVMLDGVKYEHQHGRTNYCGPANFSMALTYWGWDGNRDVIGKVVMPVNEKDKNVM
                     PYELQDFITNNVPGMTSIIRNGGDFETLKRLISAGYPVITEKGIYETDLNGKFSWMGH
                     YAFVTGYDDANQVIIYQDSYQPDPETNPGPNRRMGYEAFVEGWRAFNYVFVVVFPVEK
                     QENVLALLGPLADETQANRHALEVAKAESQTLYGIDQYFAWFNIGTSHVDLLEYADAA
                     SAYDYAFSLYADLNVDDTARPYRMMWYQTGPYKAYF"
BASE COUNT          613 a          598 c          542 g          508 t
ORIGIN      
        1 gatcgaactc tcgaccgtaa tggaaaccga gatcaacctg ccgtacatca cagcagatgc
       61 ttcgggtcca aaacacttac agttgaagct cagccgctct aaatttgagc aaatgaccga
      121 agacctgctg cagcgatgcc gtaaaccgtt tgaagcggca ctcaaagatg ccggcatgga
      181 tgctagcaaa ctcgacgaag ttgtcttagt tggtggttct tctcgtatgc caatggttca
      241 ggatctggtg cgcaagctca ccaacggcaa agacccgaac aaaggcgtga accccgatga
      301 agtggttgcc attggtgcag ctattcaagg tggcgtgctt ggtggcgatg tcaaagacat
      361 tctcttgctc gatgtgaccc cgctctctct tggtgtggaa accatgggca acgtgatgac
      421 cgtcatgatc gaacgcaata ccaccattcc tgttcgcaag accgaagttt actccaccgc
      481 tgcagataat cagaccgccg tagacattca tgtgttgcaa ggcgaacgcc caatggctgg
      541 cgataacatg agcctgggac gcttccgcct cgatggactt ccaccagcac cgcgtggcgt
      601 tccgcaggtc gaagtgacct tcgacatcga cgcaaacggc atcttgcatg tcaccgccaa
      661 agacaaagcc acaggcaagg aacagaaagt caccatcacc gcttcgacca atctcaacaa
      721 gagcgacatc gagcgcatgg tgcaagagtc caagcagaac gaagctgcag ataagaaacg
      781 ccgtgaagcg atccaagtca agaacgatgc ggacaatatg acgtatcaga ttgagaaggc
      841 attgcgtgat cttggcgata aagttccatc ggcggaaaag gcttccattg aaaccaaggt
      901 caatgagttg aagcaagccg cacaaggcga tgacatcgag cacatcaaga agctgaccga
      961 agaattgcag aacaccttcc acgcactcag ccagcagatg tacgctcaag gtcagggagg
     1021 gcaaccccag cccgagggcg gtcccgcgcc ggatggcaac gtcgtcgacg gtgaagtacg
     1081 agagtaaaaa taatcagaac tccgaagcct cagaaacttc ggagttcttt ttgtatgtat
     1141 aatctttaca tgaaaaaaag ttttaaccgt ctttggttta ttcccatttt cattgtcctc
     1201 agtattggcg tttattatct tccgcctgtt cacagccgcc ttgcatggcg tattgaactc
     1261 gtgcgcacgc aaatcaaata ttggctcaac ccgcccgatg aagctgtctt tcaacccagc
     1321 ggagaaacca gcccattgac cgtcgagaca gcggttgcca caacgcgtgc tgaattttta
     1381 ttgacgttga cgcccacctc caccccgacg ccagaactgg ttgccaccat tgagccgacc
     1441 attacatcca cacctttacc cactactgtc atgctcgatg gcgtgaagta tgaacaccaa
     1501 catgggcgga caaactactg cggacctgca aacttctcta tggcgcttac ctattggggt
     1561 tgggatggca accgcgatgt gatcggcaaa gttgtaatgc ctgtcaatga aaaagataag
     1621 aacgtgatgc cctacgagtt gcaggatttc atcacgaaca acgttcccgg tatgacgtct
     1681 atcatccgca acggcggtga ctttgaaacg cttaaacgcc ttatctcggc aggctacccg
     1741 gtcattaccg aaaaaggcat ctatgaaacc gacctcaacg gcaaattcag ttggatggga
     1801 cattatgcct ttgtcaccgg ttacgatgat gctaatcaag tcatcatcta tcaagatagt
     1861 tatcaacccg acccagaaac aaaccccggt ccaaaccgca gaatgggata cgaagccttt
     1921 gtagaaggtt ggcgtgcctt caattatgtt tttgtggtgg tctttcctgt tgaaaaacaa
     1981 gagaatgtgc tggcattgct cggtccgtta gcagatgaaa cccaagccaa tcgccatgcg
     2041 cttgaggtcg ccaaagctga atcccaaacc ctttatggca ttgaccaata ttttgcctgg
     2101 ttcaacatcg gcaccagcca tgtagacttg ctcgaatacg ccgatgccgc cagcgcctat
     2161 gattatgcat ttagcttata tgcagatttg aatgttgacg ataccgcccg tccctatcgc
     2221 atgatgtggt atcagaccgg tccgtataaa gcctatttct a
//
LOCUS       DAOREF010000544         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36608_length_2255_cov_4.719545, whole genome shotgun sequence.
ACCESSION   DAOREF010000544 DAOREF010000000
VERSION     DAOREF010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36608_length_2255_cov_4.719545"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PKI33_11120"
     CDS             <1..694
                     /locus_tag="PKI33_11120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM37608.1"
                     /translation="EGADLVVTGRRIILGKAVEAECRQKGVRCVFVEADHSKVEDCQR
                     VVETALKEFNRIDILFNNAGIVTKGTAETTSDETWQNTLNINVTAVWKMCQLVLPHMK
                     KQGKGVIVNNGSDWSVVAGKNAFPYIMSKGAVGMMTKAMALDYARDGIRVNAVCPGDT
                     FVDRWMEKGYFEGSDPVKIEDAIKEASEYLPMGRFGKPEEIANAVVFLASDEASFVTG
                     HLLLVDGGNTAQ"
     gene            747..1406
                     /locus_tag="PKI33_11125"
     CDS             747..1406
                     /locus_tag="PKI33_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNM37609.1"
                     /translation="MIVVSDMMGTLTTGSPFRGLVDWVKHNQSKARANWYLASIAPSY
                     FLAKKGVLDWQDWGHRLMINSLSYIKDATPENVAHAAEWVVEKDLWVKRREDVIARLT
                     KHREEGASIYIASSYFEPFLEPFAKRIGAKGIGTPVELVNGRLRMTSKQMVADERKLE
                     QVLSRLGVERVDVAYGDTVLDVPLLEHANHPVAVYPDVKLKKIALERGWEIIGDTPKY
                     E"
     gene            1445..2188
                     /gene="budA"
                     /locus_tag="PKI33_11130"
     CDS             1445..2188
                     /gene="budA"
                     /locus_tag="PKI33_11130"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNM37610.1"
                     /translation="MERTTIRRSRKRRADSKIYLCAPVNSLVEGIYEQNIPFHEIRKH
                     GDMGLGTFNDLDGEMMMVDGEIYRVGSDGKVNLITDDDTCTPFACVTFFQPASDEVLE
                     KEMDYEQFLRWMMELMPSPNIFYAFRITGEFAYVKTRSVPKQENYRPLVEVAKEQPTF
                     EFHNVSGKMVGFYTPSFVSALSVPGVHLHFLTEDKTSGGHLLACHPRKVKVELQYLYT
                     VELSLPANLDYLTWDFQRDIGKDLDKAEK"
BASE COUNT          604 a          422 c          640 g          589 t
ORIGIN      
        1 agaaggcgcg gatttggtgg ttacgggtcg acgcatcatt ttgggaaaag ctgtcgaagc
       61 agaatgcagg caaaagggag tccgatgcgt gttcgtagaa gcagatcact ccaaggtaga
      121 agactgtcag agagtcgttg aaactgcact taaggaattt aatcgcattg atattttgtt
      181 taacaatgct ggaattgtga cgaagggaac ggcggagaca accagtgatg aaacttggca
      241 aaatactttg aatatcaacg tgactgcggt ttggaagatg tgtcagttgg tgttgccgca
      301 catgaagaag caaggcaaag gtgtgattgt caataacggg tcagattggt ctgtggtggc
      361 agggaagaat gcctttccgt atatcatgag caaaggcgcg gtggggatga tgaccaaggc
      421 aatggcactc gactacgcac gtgatgggat tcgtgtgaac gcggtttgcc caggggatac
      481 ctttgtagat cgctggatgg agaaaggtta ctttgaaggg tcagaccctg tgaaaattga
      541 agatgctatt aaagaagcca gtgagtattt gcctatgggg cgttttggaa aaccagaaga
      601 gatcgctaat gcggttgtgt tccttgcttc tgatgaagcg tcttttgtga cgggacattt
      661 gctcttggtc gatggtggca atacagcgca gtgatatact gcaaaagttt ttaagtatat
      721 agttgaaagt tttagaagga gtttaaatga ttgttgtttc cgatatgatg ggcaccttaa
      781 ccacgggttc cccttttcgt gggttggtgg attgggtaaa gcataaccaa agtaaagctc
      841 gtgccaactg gtatctggcg tcgattgcac cttcgtattt tctggcaaag aaaggagtac
      901 ttgattggca ggattggggg catcgtttga tgatcaacag cttgtcatat atcaaggatg
      961 ccacccccga aaacgtagct catgccgccg aatgggtggt ggagaaagac ttgtgggtca
     1021 agcgtcgtga agatgtgatc gcccgtttga cgaagcatcg cgaggaaggt gcatccattt
     1081 atattgccag cagttacttt gaaccctttc ttgagccctt cgccaagcgc attggcgcaa
     1141 aagggatcgg tacacctgtg gaacttgtaa atggaagatt acgcatgacc agcaagcaga
     1201 tggtggcaga tgaacgtaaa ctggaacagg tgctttcccg cctcggcgtg gagcgggtgg
     1261 atgtggcgta tggcgatacc gttttagatg tcccattgtt ggaacatgcc aatcaccctg
     1321 tggcggtgta ccccgatgta aaattgaaga agatcgcact tgaacgcggt tgggaaatta
     1381 tcggcgatac gccaaaatac gaatgatctt gtacggaaca gcttatagag gagaatacat
     1441 tcaaatggaa cgaacaacca tacgcaggtc caggaagagg cgcgcagata gcaaaatcta
     1501 tttatgtgcg ccggtcaatt cactcgtcga aggcatttat gaacagaaca tccctttcca
     1561 tgaaattcgc aaacatggcg acatgggctt gggaacgttt aatgacctcg atggcgagat
     1621 gatgatggtg gacggtgaaa tttatcgtgt ggggtcagat ggcaaggtca atctcatcac
     1681 agacgatgat acctgtacgc catttgcctg tgtgaccttc tttcaacccg ccagtgacga
     1741 agtgctcgaa aaagaaatgg attacgagca gttcctgcgt tggatgatgg agttgatgcc
     1801 atcgccgaat attttttatg cctttcgcat cacgggtgaa tttgcgtatg tcaaaacgcg
     1861 ctcggtgccg aagcaggaga actaccgtcc actggtggaa gtggcgaagg aacaaccgac
     1921 ttttgaattt cataatgtga gcggcaaaat ggttgggttt tatacgccgt ctttcgtctc
     1981 tgcgctgagt gtgccgggcg tgcatttgca cttcctgacc gaagataaaa ccagcggtgg
     2041 acatttgctg gcatgtcatc cacgtaaagt gaaggtggag ttgcaatatc tttatacggt
     2101 agaactttca ttgcctgcaa atcttgatta tctcacttgg gatttccagc gtgatattgg
     2161 taaagacttg gacaaagcag aaaaatagag gttgagatga aaagaccaaa ctggatggtt
     2221 gttggaattt tgtttggcgt aagtttgttt ttagg
//
LOCUS       DAOREF010000545         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36636_length_2254_cov_5.921328, whole genome shotgun sequence.
ACCESSION   DAOREF010000545 DAOREF010000000
VERSION     DAOREF010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36636_length_2254_cov_5.921328"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PKI33_11135"
     CDS             complement(<1..1268)
                     /locus_tag="PKI33_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37611.1"
                     /translation="MTKLKNHILDGVISAIAGFVISFALAQRFFDLTSLPLIDQLFLV
                     FIPAVAVFICLLFLLPLLKKALEGIYTPACVFLVLWAALSSLLTLFVVHESLLFFLFL
                     FFVFLLFVIPSARSIQSLIEASGRRRVFGAWLFAFIASSLLIGFLDNFYFTTVEVILL
                     TVVLQAVFGVGGYFLMGRVRRVANERWFDALIHGVLFVLLIGFIVWLFQMSRMVSLFP
                     ISYFVLNDETSGLFLFVSLLALPWQAWFHLKLKFSGFYNRAKQTKIYSYVSANLAGFS
                     LALVFFILYLIFASVINDPRFDVDDIFFDADGLNYRIRLTTDTWHDFYWRSVHPFMIL
                     LFKPPVDLISFFLKGNKLWGAYVFVALGGAACVYLAWMFIKSATGNSVYASLIAAMLG
                     LTASHLIFGSLLESYIFLAASLLLFYVLLL"
     gene            complement(1319..1612)
                     /locus_tag="PKI33_11140"
     CDS             complement(1319..1612)
                     /locus_tag="PKI33_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37612.1"
                     /translation="MSNSQDPNTETIAETENFMAWRADEPDGETTYYLQLGRATVNFF
                     MEEWDELLASVPELLAAKPNEDGMYAVSFDNVDIWMDNEDWSEFKHLLNALGK"
     gene            complement(1655..>2254)
                     /locus_tag="PKI33_11145"
     CDS             complement(1655..>2254)
                     /locus_tag="PKI33_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNM37613.1"
                     /translation="HDFSRLSRELGRIWNAFLRGQIIIFFLAVIVYSIVLTTLGVSYA
                     IGLAFLAGLARFVPYVGPAINWTLLVIVAYFQQYSLFHLPDLTYTVIVLVIALVIDQV
                     FDNIINPRIMSSALKVHPAAVLVAAIIAANLFGILGVVVAAPILATAALLWNYIMRKL
                     LDLDPFPEKEVTQPPPPPGSRLMVTIRRFFRDRKLIAKK"
BASE COUNT          776 a          483 c          525 g          470 t
ORIGIN      
        1 ttcaggagca agacgtagaa cagcaacaaa ctcgcagcga ggaaaatgta agattcgagc
       61 aacgagccaa aaatcaagtg cgaggcggtt aatcccaaca tggcggcgat gagtgacgca
      121 tagaccgaat tccccgtcgc agacttgatg aacatccatg caagatacac acatgctgcg
      181 ccgcccaatg ctacgaagac atatgcaccc cacagtttgt tacccttgag gaaaaatgaa
      241 atcaaatcta cgggcggttt gaagagcaaa atcatgaatg ggtgaaccga acgccaatag
      301 aaatcgtgcc atgtatcggt ggtgaggcgg atgcggtagt tcaatccatc ggcgtcgaag
      361 aaaatatcat ccacatcaaa acgtggatca ttgatcaccg aagcgaaaat caaatacaag
      421 ataaaaaaca caagagctaa tgaaaaccca gccaagttag cacttacgta agaatatatt
      481 tttgtctgct tggcgcgatt gtaaaagcca ctgaacttca gtttgaggtg gaaccaagct
      541 tgccaaggca aagctagcag actgacaaac aagaaaagcc cactggtttc atcgttcagc
      601 acaaagtagg agatagggaa aagcgaaacc attcggctca tttgaaaaag ccagacaata
      661 aagccaataa gcaaaacaaa taaaacgcca tgtattaacg catcaaacca acgctcattg
      721 gctacgcgtc gaactctgcc catcaggaaa tatccaccca caccgaaaac tgcctgtaaa
      781 accacagtca acaaaataac ttcaacagtg gtgaaataga aattatctaa aaagccaatg
      841 agcagtgagc tggcaataaa agcgaataac catgctccaa atacacggcg acgcccgctt
      901 gcttcgatta ggctttgaat agatcgtgca gagggaatta caaagaggag aaaaacaaaa
      961 aagagaaaga ggaaaaagag caaagactcg tgaaccacaa acaaagtaag gagcgatgaa
     1021 agggctgccc aaagaacaag aaatacacac gcaggtgtat atattccttc aagtgctttt
     1081 ttcaggagtg gcaacaaaaa taaaagacaa ataaataccg cgacagccgg aataaacact
     1141 aaaaataatt ggtctatcaa tggcagggaa gtcaaatcaa aaaatctctg cgcgagcgca
     1201 aaggaaataa cgaacccagc tatggcgctt atcacgccgt ctaggatatg gttttttagt
     1261 tttgtcatga caaaaattaa aaggtggcag tcattctatg tgactgccac ctaattaatt
     1321 acttccctaa tgcgttcagc aaatgcttga actcagacca atcttcatta tccatccaaa
     1381 tgtccacgtt atcgaacgag acggcataca tgccatcttc gtttggcttg gctgctaata
     1441 attcagggac agacgcaagg agttcatccc attcttccat gaagaagttg actgtggcac
     1501 gacccagttg aagataatag gtggtttcac catcgggttc gtctgcgcgc catgccatga
     1561 agttttcggt ttcggcgatc gtttcggtat tggggtcttg agagttgctc atagggtctc
     1621 ctagggttta gaaaaagttt gggtgagaaa aaaattattt tttggcaatc agcttccggt
     1681 cacgaaagaa gcggcggatc gtcaccatga gccgtgaacc gggaggcgga ggtggttggg
     1741 tgacttcctt ttcagggaag ggatctaaat ccaacaattt acgcatgatg taattccaca
     1801 ataatgcggc agtagcaagg atgggcgctg caacgacaac accaagtatg ccaaacaaat
     1861 ttgctgcaat gatggctgcc actaacaccg ccgcaggatg taccttcaat gcgctagaca
     1921 taatgcgtgg attgatgatg ttatcgaaaa cttgatcgat cacaagagcg ataacaagta
     1981 cgataactgt ataggtaaga tctggcaagt ggaaaaggga atattgttgg aagtacgcca
     2041 caatcacaag caaggtccag ttgatagcgg gaccgacata aggaacaaag cgggcaagcc
     2101 ctgcaagaaa cgccaaacca atagcataac ttacaccaag tgtggtgaga acgattgaat
     2161 aaacgataac cgctaaaaag aagataatga tctgtccgcg caggaaggcg ttccagatgc
     2221 gccctagttc gcggctgagg cgggaaaaat cgtg
//
LOCUS       DAOREF010000546         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36656_length_2254_cov_2.596635, whole genome shotgun sequence.
ACCESSION   DAOREF010000546 DAOREF010000000
VERSION     DAOREF010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36656_length_2254_cov_2.596635"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..735
                     /locus_tag="PKI33_11150"
     CDS             76..735
                     /locus_tag="PKI33_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNM37614.1"
                     /translation="MADDELTMKLDGKDVSFTQGETVFEIAQRNEVFIPTLCFDPRLK
                     PFGSCRMCAVEVKGMRNPVASCTTAATPGMEVSTSTEVLEKHRKTLLEMVVSENRELE
                     VSPLRGVASQELSILVEKYGAKTGRFKGAQSGHSKLDDKNPFILRDYDLCISCYRCVR
                     VCAEQEGDYAISVMNRGFHTQITTEFNLELRSSACTFCGQCVQTCPTGALADKKALRH
                     A"
     gene            796..>2254
                     /locus_tag="PKI33_11155"
     CDS             796..>2254
                     /locus_tag="PKI33_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1246..1248,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNM37615.1"
                     /translation="MALLQELRELPLINSGVKKTRTVCGYCGVGCSVDILSKDDRIIG
                     LQPAMDGPANLGALCVKGQFAYDYVHHPDRLTKPLVRREDGNLHETTWDEALQVAAEG
                     FRKVEQKYGRHAIYGVSSGRTPTEADYLMQKFIRAGFGTHNIDNCSRAUHAPTVAGLA
                     ATIGRGAMSNPLSDLEKADVYFCIGTNMTECHPVAATRMKKALARGSKLIVADPRYIK
                     LAEMSDLYLPLRVGTDVALLLGMAHVIVRENLLDHKFIEERTSGIDEYIAHVKQFTPE
                     WASEITGVPAKDIEKAAILYASADKASIYYTLGITEHICGVDNVQSLSNLALMTGHLG
                     REGCGINPLRGQNNIQGAGDAGTSPGDYPGYQSVLKSEMREKFSKAYGREMDAEKGIT
                     KVTALEMCGEGIHAMLIDGENTVVSDPDRAHSERALGKLDFLCVIDIFLTETAEMADV
                     VFPATAWGETDGTFASTERRIQRVRAAVTPPGEAKP"
BASE COUNT          549 a          591 c          608 g          506 t
ORIGIN      
        1 gcaccgttgg tgatcgaaag cttgatcaaa caattccctg cccgcgtgca agaatttgta
       61 gagaagaggt gagccatggc tgatgatgaa ctcaccatga agttagacgg taaggatgtt
      121 tcgttcaccc aaggcgagac agtttttgag atcgctcaac gcaacgaagt attcatccca
      181 accttgtgct ttgacccgcg actcaagcct tttggttcgt gccgcatgtg cgccgtggaa
      241 gtgaaaggga tgagaaaccc agtcgcttcg tgtacaacgg ctgccacccc tggcatggaa
      301 gtaagcacca gcacggaagt gttggagaag caccgcaaga ctctgctgga aatggttgtt
      361 tcagaaaacc gtgaactgga agtttctcca ttgcgcggcg ttgcttcgca agaactttcc
      421 atccttgttg aaaagtacgg tgcgaaaacc ggtcgcttca agggcgcgca atcaggtcac
      481 agcaagcttg atgacaagaa tccattcatc ttgcgcgatt atgatctgtg catttcctgc
      541 tatcgctgtg tgcgcgtctg tgccgagcag gaaggcgatt acgccatcag cgtaatgaac
      601 cgcggcttcc atactcagat cactaccgag ttcaatttgg aattgcgttc ttccgcttgt
      661 acgttctgcg gtcaatgcgt gcagacttgt cctacgggtg cattggcaga taaaaaagcg
      721 ttacgacatg cttagtttat acgctctgtc tgacagagca gtatttattg tttcaaagta
      781 agaaggagta aagctatggc actattacaa gaattacgtg aactgcccct catcaactcg
      841 ggcgtcaaaa agactcgcac cgtttgcggg tattgcggcg tgggttgctc ggtggatatc
      901 ctcagcaaag atgaccgcat tatcggcttg cagcccgcga tggacggtcc tgccaatttg
      961 ggcgcgctgt gcgtgaaggg acagtttgcc tatgactatg tccatcaccc agatcggttg
     1021 accaaaccgc tcgtgcgtcg tgaagatgga aacttgcatg aaactacttg ggacgaagct
     1081 cttcaagtcg cagcagaggg tttccgcaaa gtggagcaga agtatgggcg tcatgccatt
     1141 tacggtgtct ccagcggacg cactcccacc gaagccgatt acctgatgca gaaattcatc
     1201 cgtgcgggct tcggcaccca taatatcgat aactgcagcc gtgcctgaca cgcgcccact
     1261 gtcgccggtc tggcggcaac cattggtcgc ggtgccatgt caaatccatt atctgacctt
     1321 gaaaaggcag atgtctattt ctgcattggc acaaacatga cggaatgcca cccagtggcg
     1381 gcaacccgta tgaagaaagc attggctcgc ggatctaaat tgatcgtcgc ggatccgcgt
     1441 tacatcaaac tggcagagat gtctgacctc tacctgcccc tgcgcgtcgg tacggatgtg
     1501 gcgttattgc tcggcatggc gcatgtcatc gtccgcgaga atttgcttga ccataaattc
     1561 atcgaagaac gcaccagcgg cattgatgaa tacattgcgc acgtcaaaca gttcacaccc
     1621 gaatgggctt ctgagatcac aggcgtacct gccaaggata ttgaaaaagc cgccattctg
     1681 tatgcgagtg ccgacaaagc atccatctat tacacactcg gcatcaccga acacatctgc
     1741 ggcgtggata acgtccaaag cctgagcaat ctcgctctga tgacaggtca tttgggacgc
     1801 gaaggctgcg gcatcaatcc gctgcgtggg cagaacaaca ttcagggcgc gggtgatgcg
     1861 ggcacatccc ccggagatta tcccggctat caatcggtgc ttaagtctga gatgcgtgaa
     1921 aagttctcga aggcgtatgg tcgcgaaatg gatgccgaga agggcatcac caaagtgacc
     1981 gcgctcgaaa tgtgcggcga aggcatccac gccatgttga tcgacggcga gaacaccgtg
     2041 gtctctgacc cagaccgcgc tcacagcgaa cgcgctcttg gcaagttgga tttcctttgc
     2101 gtgatcgata tcttcctgac cgaaaccgca gaaatggcgg atgtggtttt tcctgccacc
     2161 gcatggggcg aaacagacgg aacctttgcc agcaccgaac gccgcattca acgcgtgcgt
     2221 gcagcggtca ctccgccggg agaagccaaa cccg
//
LOCUS       DAOREF010000547         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36737_length_2250_cov_3.843280, whole genome shotgun sequence.
ACCESSION   DAOREF010000547 DAOREF010000000
VERSION     DAOREF010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36737_length_2250_cov_3.843280"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKI33_11160"
     CDS             complement(<1..941)
                     /locus_tag="PKI33_11160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM37616.1"
                     /translation="MTRICITPFVQGTGGMASFRLKFEQGLHARNIDVTYDLDDKSDA
                     VLVIAGTRFLLDLNRARRRGIRVVQRLDGINWVQRVKWSGVKYTVRAEYGNFMLSLIR
                     NRFADRVIYQSQFIRKWWEDWYGVAKAPASVIINGVDLNAYTHEGEHERPTDTYRMLL
                     LEGSLARGLNSGLFHAVSVAEKMSAKYPMEVVVAGVVDEATQQKLQSKVPVKFLGTVP
                     RADIPKLARSSHFMYCAEVNPPCPNSVIEALACGLPVIGFDSGSLKELVTEDAGCIVP
                     YGANPWKLETPDIKTLASSAGEVLEKQNQFRAAARRRA"
     gene            1030..1662
                     /locus_tag="PKI33_11165"
     CDS             1030..1662
                     /locus_tag="PKI33_11165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNM37617.1"
                     /translation="MYIAIEGVIGVGKTTLARLLQPAFEAEVLLEVFEENPFLSDFYG
                     DRARYAFQTQIFFLLSRYHQQNNNVPNILAAGKNLISDYTFAKDALFAGINIKGDELQ
                     MYHRVHEALGEKIPKPDLLVYLQATTDTLMSRIAFRDRPYERQMERAYIDELNVAYDE
                     FFSKPFDHTPILKIDTNDLNIIHNAEHIKLIENRIREALGLSPYQPALLK"
     gene            1672..>2250
                     /locus_tag="PKI33_11170"
     CDS             1672..>2250
                     /locus_tag="PKI33_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37618.1"
                     /translation="MRVTKDSLLRIAKETVQERAYNDPDIVAAYLTGSLRGEPMLGGT
                     ADIDIVFVHKTTPMKVREFVKLTPDFHIDISRRAKDEFKSPRELRGDPWLGYEMYDPV
                     LLYEREKFFDFVQASLRAGFEFEQPPLMLQRCRTLLNHGRGNWMDISEHDGEVGPKEI
                     RKYMKALFHAVNAIAELSGPPIWERRLLLDFPA"
BASE COUNT          535 a          564 c          567 g          584 t
ORIGIN      
        1 gcccgcctcc gtgccgctgc tcggaattga ttctgtttct caagcacctc tcccgctgac
       61 gatgccagtg tcttaatatc tggcgtctcc aacttccatg ggttcgcgcc gtacggaacg
      121 atacagcctg catcctccgt aaccaactct ttgagcgagc cgctgtcaaa accgatgacg
      181 ggtaaaccgc atgcgagggc ttcgatgacc gaattggggc agggcgggtt gacctcggcg
      241 cagtacatga aatgagaaga gcgggcgagt ttgggaatgt ccgctcgcgg gacggtgccg
      301 agaaatttaa caggcacttt gctttgcagt ttttgttgtg tagcttcatc cactacaccc
      361 gcgacaacaa cttccatcgg atacttcgca gacatttttt ccgcgacgga aacagcgtgg
      421 aacaagcctg agtttaatcc acgagcgagg ctgccttcga gtagaagcat tcggtatgta
      481 tctgttggac gttcatgctc gccttcatgc gtgtaagcat ttaggtctac gccattgata
      541 atgaccgaag ctggcgcttt ggcgacgcca taccagtctt cccaccattt gcggatgaat
      601 tgactttgat aaatgacccg gtccgcaaag cgattgcgga tgagggaaag catgaaattc
      661 ccatactccg cgcggacagt gtatttgacc cccgaccatt tcacacgctg cacccagttg
      721 atgccgtcta acctttggac gacgcgaatg ccccgtcgcc gtgcccggtt caggtccagt
      781 aagaagcggg tgcctgcaat gacgagcacc gcatcagatt tgtcatctag atcataggtc
      841 acgtcaatgt tgcgggcgtg taatccctgt tcgaatttca aacggaagga tgccatgccg
      901 cctgtccctt gaacgaatgg cgtgatgcaa atgcgagtca tatacaaaat tataaccgta
      961 ggggcgaggt cacctcgccc ttacttttat ggtatcctat gttcaatcat tttttgggag
     1021 aatttgttca tgtatattgc aattgagggc gtgatcggtg tagggaagac tacacttgcg
     1081 cgcttgctgc aacctgcttt tgaagcagaa gtgttattgg aagtttttga agagaatccg
     1141 tttctctcag atttttatgg cgaccgcgca cgttatgcat ttcaaaccca gatcttcttt
     1201 ttgctcagtc gctaccatca acagaacaac aatgtgccca atattcttgc ggctggaaaa
     1261 aatttgatct ctgattacac ctttgccaaa gatgcgcttt ttgcaggcat caacataaaa
     1321 ggtgacgagt tgcagatgta tcacagagtg catgaggcgc ttggcgagaa aattcctaaa
     1381 cccgaccttc tggtttatct gcaagcgacg accgatacgt tgatgagccg tattgcattt
     1441 cgcgaccgcc cgtatgaacg ccagatggaa cgtgcctata tcgatgaatt gaatgttgcg
     1501 tatgatgaat tcttctcgaa gccgttcgac catacgccga ttttgaaaat tgatacgaac
     1561 gatctaaata tcatccataa tgcggagcat atcaagttga tcgaaaatcg catccgtgaa
     1621 gctttggggc tgtcacctta tcagcccgcc ttgttgaaat aggcaggacc catgcgcgtt
     1681 acaaaagact cccttttacg tattgccaaa gaaaccgttc aagaacgggc gtataacgat
     1741 cccgatattg ttgcggcata tctcaccggc tcgttgcgcg gcgaacccat gcttggcggc
     1801 actgccgaca ttgacattgt cttcgtccac aaaaccacac cgatgaaggt tcgtgaattc
     1861 gtaaaactca cgcccgattt tcatattgat atatcccgcc gtgcaaagga tgaattcaaa
     1921 tctccgcgtg agttgcgcgg tgatccgtgg ctcgggtatg aaatgtatga cccggttctg
     1981 ttgtatgaac gtgaaaagtt tttcgacttt gtgcaagcca gtctgcgtgc tggcttcgag
     2041 tttgagcagc cgccgttgat gctgcagcgt tgccgcacac ttctcaacca cgggcgcggc
     2101 aactggatgg atatttcgga acacgatggg gaagtgggtc cgaaagaaat tcgcaaatac
     2161 atgaaggcgc tttttcatgc ggtcaatgcc attgctgagt tgagcggtcc gcccatttgg
     2221 gagcgccgcc tgctgctcga tttccctgct
//
LOCUS       DAOREF010000548         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_36880_length_2245_cov_5.411872, whole genome shotgun sequence.
ACCESSION   DAOREF010000548 DAOREF010000000
VERSION     DAOREF010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36880_length_2245_cov_5.411872"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1837
                     /locus_tag="PKI33_11175"
     CDS             242..1837
                     /locus_tag="PKI33_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNM37619.1"
                     /translation="MKNNPNKLWIFVLALGWLFDFLFWKQAPGVNFAIFWTACLIVGF
                     YLLLSNGHSPHRTTLLLIPLFGFFAAVTFIRAEPMTTFLAYTFTMFTLTIFTVTYLGG
                     RWVQYALADYVGKLLSLVGSMLIRPITFTADVRKTQAEAGTQPSKYNFMPIVRGLVIA
                     IPIVMIFASLLASADVVFNQRLDDFVQIFKLENLPEYIFRMIYILVIGYALAGIFLHA
                     STESKDEKLVGEDKPVIPAFLGFIESSIVLGSVIALFAAFVVIQFQYFFGGTTNINVE
                     GYTYAEYARKGFGELVAVAFFALMMLLTLTGVTKRETETQRKTYSGLGIALVALLLVM
                     LVSAYQRLNLYEAAYGFSRLRTYTHVFLIWIGLLLITTIVLEILRKERMFTFAMLIAS
                     FGFAASLPILNVDAFIVNQNIQRELSGNDVEDLDSQYFIELSDDAVPPLVKALQTPSL
                     PDSVHEKVTAALACIRYQRDLQRNDREYSWQSFQFSRLNADNALKSVQAKIDVFELDE
                     SEYPTKAIAPSGEEYPCSQYYYD"
     gene            1965..>2245
                     /locus_tag="PKI33_11180"
     CDS             1965..>2245
                     /locus_tag="PKI33_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNM37620.1"
                     /translation="MVERLKKEYNVEVEWRPYYLRPDTPPEGMELPDYIKRARDNGSE
                     ERLRMMAEMHGMPFRSTDRIYNTRLAHEATEYAREHGKANEFHHIMFRKV"
BASE COUNT          544 a          619 c          459 g          623 t
ORIGIN      
        1 gaattcaatt tgtgtgcccc cgcctggtga gcaaaagtat gtcataaatc aaattggatg
       61 tcaatagggc gattttcgat tctcacatat ttgtaatttt tgcctttgcc gctccctgta
      121 atttgccaca attttcaaat ttctgatacc ttttgagagt ctccgcaagc cttgcagggc
      181 tatcctcggg gaaacttttt accgcttccc tatatcgtgg taaaaataat ttggagccct
      241 tatgaaaaat aaccctaata aactctggat ctttgtcctc gcccttggct ggctattcga
      301 cttcctcttc tggaagcaag ctcctggcgt caacttcgcc atcttttgga cggcgtgcct
      361 catcgtaggg ttctacctct tactcagcaa tggacatagc ccgcatcgca cgaccttatt
      421 gctgattcca ctctttggct tcttcgccgc tgtcaccttc atccgcgccg aaccaatgac
      481 caccttcctt gcttacacct tcaccatgtt caccctgacc attttcacgg tcacctatct
      541 cggcgggcgt tgggttcaat atgcattggc agactatgtc ggaaagttac tttcacttgt
      601 cggcagtatg cttattcgcc ccatcacctt tactgccgat gtacgtaaaa cccaagccga
      661 agcaggcaca caaccttcca aatacaattt catgcccatc gtacgcggac ttgtgattgc
      721 cattccgatc gtgatgatct tcgcctcgct tctggcatct gccgatgtgg tcttcaacca
      781 gcgtctcgat gattttgtgc aaatctttaa actagagaat ctgcccgaat acatcttccg
      841 catgatctac attctggtca ttggctatgc cttggcaggt attttcctcc atgcctccac
      901 cgaatcaaaa gatgaaaagc tagtcggcga agataaaccc gtcatccccg ctttccttgg
      961 tttcatcgag tcgtcaatcg tacttggcag tgtcatcgca ctcttcgcag cgtttgtggt
     1021 cattcaattc cagtatttct ttggcggcac gaccaacatc aatgtggaag gctataccta
     1081 tgccgaatac gcccgcaaag gttttggcga acttgtagct gtagcatttt ttgctttgat
     1141 gatgctgctc accctcactg gcgtcacaaa acgcgaaaca gaaacccagc gcaaaactta
     1201 ttctgggctt ggcattgcac tcgttgcact tttgctggtg atgttggtct ctgcatatca
     1261 acgcctcaac ctctacgaag ccgcttatgg cttctcacgc ttgcgcactt acacccacgt
     1321 cttcctaatc tggatcggct tactgctcat caccaccatc gtgcttgaaa ttttgcgcaa
     1381 agagcgtatg ttcacttttg caatgctgat tgcatcgttt ggctttgcag cctctctgcc
     1441 catcttgaat gtcgatgcct ttatcgtcaa ccaaaatatc caacgcgaac taagtggaaa
     1501 tgacgtagaa gatttggact ctcaatactt cattgaactc tccgatgatg ccgttccacc
     1561 tctcgtcaaa gcgcttcaga ctccgtccct gcctgactcc gtccatgaga aagtgaccgc
     1621 cgccctagcc tgcatccgct accagcgcga tttgcagcgt aatgaccgcg agtattcatg
     1681 gcagtccttc caattttcgc gcctcaatgc agataatgct ctgaaatctg tccaagccaa
     1741 aattgacgta ttcgaactcg acgaatctga ataccccaca aaggcaatcg caccaagcgg
     1801 cgaagaatat ccctgctcac agtattacta cgattaatgc taggtaagaa tgctgtttca
     1861 ttgaatgtct gtcaatcatc aatcacacaa aaggacatcc aatggacttc aaactaacca
     1921 tcttctccga ttacatatga ccctggtgct atgtcggcca gggtgtggtc gagaggctta
     1981 agaaagaata taacgtggag gtggaatggc gtccgtacta tttacgcccc gatactccgc
     2041 ccgaaggcat ggaattgcct gattacatca aacgagcacg tgacaatggc tcggaagaac
     2101 gcctgcgaat gatggctgaa atgcacggta tgccatttag gtctacagac cgaatttaca
     2161 acacacgtct cgcccacgaa gcgacagaat atgcccgcga acatggcaaa gccaatgagt
     2221 tccatcacat catgttccgc aaggt
//
LOCUS       DAOREF010000549         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37107_length_2237_cov_5.090742, whole genome shotgun sequence.
ACCESSION   DAOREF010000549 DAOREF010000000
VERSION     DAOREF010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37107_length_2237_cov_5.090742"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /locus_tag="PKI33_11185"
     CDS             <1..1954
                     /locus_tag="PKI33_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37621.1"
                     /translation="GKTDEALALLKEGLTKVPIAGSASLYPRTADLLVQTGKTDEALA
                     LFKEGLTKLPVESLDTIYLRTADLLVQTGKTDEALALLKEGLTKVPVESLSSLYQSAS
                     ELLTLVGKPDEAIAWLKDGISKISTNHPSSAGLVEFVFAILVSQEDLEAFDKFLIETE
                     TLNRLAQSVAYGRILRLILMHNWELAAHVAHQERTNFPQYRPIMLYDIFLWLTFNNLN
                     AAQEALKRYPSPTEYDPLNWLGTWIALKRGDQVTARYYLLKYLNRHVEERELNEQFLL
                     DLWDTAPIFGSRRDLPIFFPILPALLTNLPNDVVRKQYGPSIIKSQIGNRTEVQGEQD
                     ITQTEVLSQQKNDQPTWPMWMDDLLNSSETKERADIGIVIALEEEFRELAPQINFKAY
                     YNQDIKQYYYLFERGIVNQGQTPYHCVATFMGAMGPTDAGIIGDRLISQFNPKTIISI
                     GIAGSTDKDVLVGNVVVADQTDEYLSSSKAIETIDKQDWDFQLSGNPFKSDPTYVAHA
                     MNLKYAHKVKMQEWERNCKQNLLEKIGLVAIENLIREQLIGDFPDIQTGHIASGPTVG
                     AAKQFVQWLKNKHDRKLLALEMESAGVLNAAHKRAVSSLIIRGISDYSDERKDKLDGI
                     GQGVLRRYAMNNALSLLWLLMDLGLT"
BASE COUNT          694 a          500 c          458 g          585 t
ORIGIN      
        1 aggcaaaacc gacgaagccc tcgccctcct caaggaaggt cttaccaaag tacctatcgc
       61 gggttcggca tctctttacc cacgaaccgc cgatctgctg gtacaaacag gcaaaaccga
      121 cgaagccctc gcccttttca aggaaggtct caccaaactc cctgtcgaga gtttggatac
      181 tatctaccta cgaaccgccg atctgctggt acaaacaggc aaaaccgacg aagccctcgc
      241 cctcctcaag gaaggtctta ccaaagtacc tgtcgagagt ttatcttccc tgtatcaaag
      301 tgcaagtgaa ttactaacac tggtgggcaa gccagatgaa gctatcgctt ggctcaaaga
      361 tggtatttcc aaaatatcaa ccaatcatcc ctcatccgca gggcttgttg aatttgtatt
      421 tgcaatattg gtttctcaag aagaccttga agcatttgac aagtttctaa tcgagaccga
      481 aacattaaat agacttgctc aatctgtagc ttatggcaga attctgcgat tgattctaat
      541 gcacaactgg gaattagccg ctcatgttgc tcatcaggaa agaacaaatt tcccccagta
      601 tcgtccaatt atgttatatg atatcttttt atggctcact ttcaataact tgaacgcggc
      661 tcaagaagct ctgaaaagat acccctctcc aacagaatac gatccgctga attggctcgg
      721 aacttggatt gctctaaaaa gaggagatca agtcactgcc agatattact tgctcaaata
      781 cttaaatcgg catgttgaag aacgtgaatt aaatgagcaa ttcctgttag atttgtggga
      841 tactgctcca atctttggtt ctcgaaggga cctaccaatt ttctttccca tactacctgc
      901 tctacttact aacttgccta atgatgtagt tcgtaaacaa tatggcccca gtataatcaa
      961 atcccagatt gggaatagaa cagaagtaca gggcgaacaa gatatcaccc aaactgaggt
     1021 cctctcccaa caaaaaaatg atcaaccaac ttggcccatg tggatggatg atttgctaaa
     1081 ttcttccgaa actaaagaac gtgcagatat cggtattgta attgcattgg aggaggagtt
     1141 ccgtgaacta gctccgcaaa taaatttcaa agcttactac aatcaagata tcaagcagta
     1201 ttactacttg tttgagcgcg gtattgtaaa tcaaggacag acaccatacc attgcgttgc
     1261 gacgttcatg ggggcaatgg gaccaacaga cgcaggcata ataggagatc gcctcatttc
     1321 tcagtttaat ccaaaaacca ttataagtat tgggattgct ggttctacgg ataaagatgt
     1381 tttggttggc aatgttgtcg tagccgacca aactgatgaa tatttgtctt ctagcaaagc
     1441 tattgaaaca attgataaac aggattggga ttttcaactc agcgggaatc cctttaaaag
     1501 tgaccccact tatgttgcac atgccatgaa tttaaaatac gcacataaag ttaaaatgca
     1561 ggagtgggaa agaaattgta agcaaaattt actagagaag attgggttag ttgctattga
     1621 aaaccttata cgtgaacaat taataggtga tttccctgat atccagacag gtcatattgc
     1681 ttcgggtcca acagtcggcg cagctaaaca atttgtacaa tggctaaaga ataagcatga
     1741 ccgcaaacta ttagcattag aaatggaatc cgcaggtgtt cttaatgcag cgcataaaag
     1801 agctgtaagt tctctaataa ttcgcggtat ctctgattat agtgatgaaa gaaaagacaa
     1861 gttagatgga attggtcaag gagtgttacg gcgttatgcg atgaataacg ctctaagtct
     1921 actctggtta cttatggatt tagggctaac atagtctttc atataggcaa ataacagaat
     1981 atatttcatt cgaagcagaa cccgcttcca gttaagaacg atgcgggttt tttttcattc
     2041 aacaaacaat catcgggtaa aataccgcca ccatgtcaaa cgaaaccaca caagtcatct
     2101 actccatgaa caaagtgggc aagatcgtcc cgcccaacaa acagatcctg cgcgacatct
     2161 cgctcggctt ctacctcggc gccaagatcg gcgtgctcgg tctcaacggc gcgggcaaat
     2221 ccaccctgct gcgcatc
//
LOCUS       DAOREF010000550         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37212_length_2234_cov_3.513079, whole genome shotgun sequence.
ACCESSION   DAOREF010000550 DAOREF010000000
VERSION     DAOREF010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37212_length_2234_cov_3.513079"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..788)
                     /locus_tag="PKI33_11190"
     CDS             complement(174..788)
                     /locus_tag="PKI33_11190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNM37622.1"
                     /translation="MKSKLFLAIPPYIAVWVGMFVLKNAWAALLGFHIAILLVVFLLK
                     PQYSFRTFFQSTSKKLFFFTILLCALSGPSLYLLRDFLGISPNLRVQLTALGLQGNVW
                     EWFILYFSIINPLMEEFFWRIVLGSDIKSFYLGDLVYAGYHTMIVWNKTSPLSIVLVL
                     LALTFIGWLWRQLYRKDGSLLTPVVSHSIADLSILLAIFFLVNP"
     gene            complement(796..1719)
                     /gene="mvk"
                     /locus_tag="PKI33_11195"
     CDS             complement(796..1719)
                     /gene="mvk"
                     /locus_tag="PKI33_11195"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNM37623.1"
                     /translation="MKSSAPGKIILFGEHAVVYNRPALAVPVNQVQVDVEVLDSDKPG
                     VWIDAPMVNLHGELSTLPSDHPIGSVILKVFQHLGIFPQPDLKLTIYSTIPVAAGLGS
                     GAAVSVALIRALSSFLNHSLSNEQVNELVFEIEKLHHGTPSGIDNTVITYNMPVYYMK
                     GKPIETFKCGKPFTIVIGDTGVPAPTKESVGDVRRLWQRDTNHFENIFNEVAQISTMA
                     RHFIESGRPEHLGELMDHNHEFLQQMTVSSPELDRLVDAARKAGALGAKLSGGGRGGN
                     MIALVNQDSAELVAAALMSAGAKRTVITEVK"
     gene            complement(1716..>2234)
                     /locus_tag="PKI33_11200"
     CDS             complement(1716..>2234)
                     /locus_tag="PKI33_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNM37624.1"
                     /translation="LNLAYSNYLKHIPILDVLIISIGFVLRVGAGVTLIAVERFSPWL
                     YIVMFLLSLFLGFGKRRAELALLAHGAGSHRKVLDGYTLPLLDQYIMIVSGTTIVAYS
                     LYTFSAPNVPENHSMMLTIPFLVYAIFRYLYLIEVEHAGGTPEEVLLSDRPFQISMLL
                     WAVTVIIIFYFS"
BASE COUNT          684 a          494 c          556 g          500 t
ORIGIN      
        1 actttctgca aaggtcacgc catggcttaa cgccacaaaa aataccaccg gacctgatgt
       61 aagtggcatc cctactatcc aaccgctaac ggcaggtccc caccggcgac ctgccaaact
      121 ggcagaccca ataatgagag gcgcaagaat tagttttaga gcaagaacat tcattaggga
      181 ttcaccagaa agaatatagc taacaagatc gaaagatctg caatagaatg gctcacaaca
      241 ggtgtgagta aacttccgtc tttgcgatac agctgacgcc aaagccagcc aataaaggtc
      301 aatgcaagaa gtacaagcac aattgatagc ggagacgttt tattccacac aatcatagtg
      361 tgataacctg catagaccag atcgcccaag taaaacgatt ttatgtcgct ccctaaaacg
      421 atacgccaaa aaaattcttc cataaggggg ttgataatcg aaaagtataa aatgaaccat
      481 tcccaaacat tgccctgtaa accaagagcc gtcagttgca cgcgaaggtt cggagaaatc
      541 cctaaaaaat cacgcagtag atataggcta ggtccactta gagcacagag taatatagta
      601 aagaaaaata attttttact cgtgctctga aaaaaagtac gaaaagaata ttgaggcttt
      661 agtaaaaata cgacaagcag gattgcgata tgaaaaccaa gtaaagccgc ccaggcattt
      721 tttaaaacaa acatccccac ccaaacagca atatagggtg ggatagctaa aaacaatttt
      781 gatttcatag gaggattact taacttcggt gatgactgtc cgttttgcgc ctgcggacat
      841 gagtgctgcc gctaccaact ccgctgagtc ttgattcacc aacgcaatca tattgccccc
      901 gcgtccgcca ccgctgagct tggcgccaag tgcccctgct tttcgggctg catccacaag
      961 cctatccagt tcaggcgaag agacggtcat ttgttgaaga aattcgtgat tgtggtccat
     1021 caactcaccg agatgctcgg gacgtccgct ttcgatgaag tgacgtgcca tggtggagat
     1081 ctgcgctact tcgttgaaga tgttctcgaa gtggtttgta tccctttgcc atagacggcg
     1141 cacatcccct acggattctt tggtgggcgc aggaacccct gtatcgccaa tcacaatggt
     1201 gaagggtttg ccgcatttga aggtttcaat tggcttgcct ttcatgtaat acacaggcat
     1261 attataggta atgaccgtat tatctatgcc tgagggcgtg ccatggtgaa gtttttcaat
     1321 ttcaaaaaca agttcattga cttgctcatt ggaaagcgaa tggtttagga aagaagataa
     1381 tgccctaatt aaagcaacgg aaactgcggc accagagccg agacctgcag caacagggat
     1441 ggtagagtag atggttagtt tgaggtctgg ctgtgggaag atgcctaagt gttgaaacac
     1501 tttcagaatc accgaaccaa ttggatggtc ggaaggaagc gtggaaagct cgccatgcaa
     1561 gttcaccatg ggggcgtcga tccaaacgcc aggcttgtct gagtctaata cttccacatc
     1621 cacttgcact tggttgacag ggacggcaag cgcaggacgg ttgtatacaa cggcatgttc
     1681 gccgaagaga atgatcttgc cgggagcgga agatttcatg aaaagtaaaa gatgataatg
     1741 acggtcactg cccaaagcag catggagatt tggaagggac gatcagaaag caaaacttcc
     1801 tctggcgtgc caccggcatg ttctacttcg atgagataaa gatagcggaa gatggcatac
     1861 accagaaatg ggatagtgag catcatggag tgattttcgg gcacattggg tgctgagaag
     1921 gtatagagcg aatatgccac aatggttgtg ccggatacga tcatgatgta ttggtcgagt
     1981 aagggcaaag tatagccatc gagcactttg cggtgagaac ctgccccatg agccaacagg
     2041 gcaagctcgg cgcgacgttt gccaaagccg aggaaaagcg aaagcaggaa cattacgata
     2101 taaagccagg gagagaaacg ctccactgca atgagcgtca caccggcgcc cacgcgcaga
     2161 acgaagccaa tagagatgat gagcacatca agaatgggga tgtgctttag gtaatttgaa
     2221 tacgcaaggt ttag
//
LOCUS       DAOREF010000551         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37217_length_2234_cov_3.096374, whole genome shotgun sequence.
ACCESSION   DAOREF010000551 DAOREF010000000
VERSION     DAOREF010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37217_length_2234_cov_3.096374"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKI33_11205"
     CDS             <1..272
                     /locus_tag="PKI33_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37625.1"
                     /translation="ELGYFFLLFVQSLVILVYGLVIRSRSLTLFPIGFVALGVITVVY
                     STLKDLGTIFVIGCTGIVLLMLGVGAVLLRERIAKLGEKLSDWKA"
     gene            291..695
                     /locus_tag="PKI33_11210"
     CDS             291..695
                     /locus_tag="PKI33_11210"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM37626.1"
                     /translation="MNTQNILELRVAITTKDYERLVKFYCLGLGIEPAAVWNNDGGKA
                     LMLNMGNATMEIFDERQAEVVDDLEVGERVSGQIRFALQVPDLNAAMERMLANGAILV
                     HEPVLTPWGDYNVRLQDPDGMQITLFQAKENG"
     gene            complement(692..1429)
                     /locus_tag="PKI33_11215"
     CDS             complement(692..1429)
                     /locus_tag="PKI33_11215"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNM37627.1"
                     /translation="MNFPADFVRFLTSATRVVVLTGAGVSQESGLRTFRDAQTGLWAR
                     YKPEDLASPEAFARDPKLVWDWYAWRREAIKGVRPNPGHYALVEMEKRIPQFTLITQN
                     VDGLHRFAGNQNVLELHGNIQKVRCSECRTFTEEWGDEMEGVPTCSVCGGLLRPDVVW
                     FGETLPRAELEAAVTASRAAQVYFSIGTSGVVQPAASLAHAARNNGSVVIEINAEPTP
                     LTPKVDFAFHGKSGEILPALIKAVWGN"
     gene            complement(1430..2113)
                     /locus_tag="PKI33_11220"
     CDS             complement(1430..2113)
                     /locus_tag="PKI33_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37628.1"
                     /translation="MRKIFLLTLLLIFSLACSLGTFAPQPTATPAPTQTDTATPLPTP
                     TPVTPTLTFTSTPTLISNTAIPTIETTSTELSTPTATQALATPYTVMPPVNIKGFISI
                     TISLTEVFKAKGCEPSVVRVTAQVANPNDVSFVLLFSRFKSLKAERTGKWTTVQMIPL
                     GAGTYIFDLSSALMKDDAYFENAWIEYQVVSTNQAGTELGRTDIFKERLKMVECIFTA
                     TPTSATVKP"
BASE COUNT          589 a          462 c          580 g          603 t
ORIGIN      
        1 atgaacttgg ctatttcttc ctgctatttg ttcagtcgct tgtaattctt gtttatggtc
       61 ttgtcattcg ttcacgcagt ctaaccttat ttccaattgg gtttgtagct ttaggtgtga
      121 ttacagttgt atatagcaca ctaaaagacc ttggaacgat ttttgtaatt ggttgtacag
      181 gcattgtcct gcttatgctc ggggttggcg ccgttctgtt acgcgaacga attgcgaaac
      241 taggcgaaaa gttgagcgac tggaaagctt gatttcaaga ggactattaa atgaatacac
      301 aaaatatttt agaacttcga gttgctatta ctacaaaaga ttatgaaaga ctcgtgaagt
      361 tctattgcct cggactggga attgaacctg ccgctgtatg gaacaacgac ggcggcaaag
      421 ctctcatgct taatatggga aacgctacca tggaaatttt tgatgaacgt caagccgaag
      481 tggttgatga cctcgaagtt ggtgaacgag tgagcggaca aattcggttt gcactgcaag
      541 taccagactt aaacgccgcc atggaacgga tgctcgctaa tggtgcgatc ctcgtccatg
      601 aacctgtgtt gacaccatgg ggtgactata atgtccggtt gcaagacccg gatggtatgc
      661 aaatcacgct atttcaggca aaagagaacg gctaattgcc ccaaaccgcc ttaataagcg
      721 caggcaaaat ttcgccagac ttaccatgaa aagcaaaatc aactttgggt gtaagcgggg
      781 ttggctcggc gttgatctct atgactacag aaccattatt gcgggctgca tgtgctaaag
      841 acgcagcagg ctgaacgaca cctgatgtgc cgatcgaaaa ataaacttgt gcagcgcgtg
      901 aagcagtgac tgccgcttca agttctgctc ttggcagggt ctctccaaac caaacaacat
      961 ctggtcgaag aagcccgcca cagactgagc aggttggaac gccttccatc tcatcccccc
     1021 actcttctgt aaacgttcgg cattcggaac aacgcacctt ttgaatatta ccgtgtaatt
     1081 ccaatacatt ttggttccca gcaaagcgat gcaacccatc cacattttgg gtgatgaggg
     1141 tgaattgggg aatccgcttt tccatttcta ctagcgcata atgtccgggg tttgggcgga
     1201 cgcccttaat tgcctcacga cgccaagcgt accaatccca aaccagtttc gggtcgcggg
     1261 caaaagcttc aggggaggct aaatcttctg gtttgtatcg agcccaaagt ccggtttgag
     1321 cgtcgcgaaa ggtgcggagt ccgctttctt gggagactcc ggctccagtg agtacgacga
     1381 ctcgtgtggc agaagtcaaa aagcggacga aatctgcagg gaaattcatc tacggtttta
     1441 cagtggcaga tgtgggggtg gcagtaaaga tacattccac catcttgagg cgttctttga
     1501 aaatatcagt gcgccctaat tctgtgccgg cttgatttgt agagacgact tgatattcga
     1561 tccatgcgtt ttcgaaataa gcatcatctt tcataagcgc agaagaaaga tcgaatatat
     1621 atgtacctgc acccagtggg atcatttgaa cagttgtcca tttaccggtg cgctctgctt
     1681 tcagactctt aaaacgggag aacaagagaa caaatgagac atcgtttggg tttgctactt
     1741 gagcagtaac gcgaacaaca gaaggctcac atccttttgc cttaaaaacc tcggtcagtg
     1801 aaatcgtaat agatataaag cctttaatat ttactggagg catcactgta taaggtgtcg
     1861 ccaatgcctg tgtggcagtt ggagttgaaa gctcggttga agtggtttca attgttggga
     1921 tagctgtatt actgatgagt gttggagttg aggtaaacgt cagagttggt gttacaggcg
     1981 ttggcgtagg aagaggcgtt gcggtgtccg tctgcgttgg cgctggggtc gcagttggtt
     2041 gcggagcgaa cgtccctaat gaacaagcaa gggaaaaaat aagaagaagc gtaagtaaaa
     2101 atatttttct catggtttat tttttaggag ttggagatgg tggttttggt gtggcgattg
     2161 ggcagccagt aagtggcgta aggcacgggc atggatacat atcaaaagct ttgtcgtaga
     2221 ttttagtgcg tcca
//
LOCUS       DAOREF010000552         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37469_length_2226_cov_5.171810, whole genome shotgun sequence.
ACCESSION   DAOREF010000552 DAOREF010000000
VERSION     DAOREF010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37469_length_2226_cov_5.171810"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKI33_11225"
     CDS             <1..233
                     /locus_tag="PKI33_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37629.1"
                     /translation="LIDESTLRKHAADAFEFHFVKRPQIESRVRIDEGQVVSSLSPLD
                     LLDQYFDAAKVSNANELKNLAQEIISEDSQDS"
     gene            286..1569
                     /locus_tag="PKI33_11230"
     CDS             286..1569
                     /locus_tag="PKI33_11230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37630.1"
                     /translation="MSKEHSRYRWFVVAVFFFFMLLHQTDKLMIGSLQVQISNDFNIN
                     NKQWGLVNSGALIVATILYPIWGYLYDRYSRTKLLSLASFIWGSTTWLNAIMKSFGGF
                     LVTRASTGIDDSSYPGLYTLIADYFGPTQRGKIFGILQLAQPIGYLLGMILALMVAPA
                     IGGWRSVFYITGSLGLVIALLIFFGVREMPRGKAEPEFENMTEMQTFKFSWAEAREIF
                     KKKTMWFVFLQGFAGVFPWNVITFFFFAYLEKERGYDANSILFTMAPVILILASGYFV
                     GGALGDYAFKRSNKGRIIVSSVGVLLGALFMYLAITTPLEAKNQFFVFMCLTAIFMPL
                     SSTNVTATVYDVTVPEVRSTAQAVEYFIENAGAAFAPIITGVIADMYDLKTAILLICT
                     VAWGLCFLFYLGAIFTIDKDANDLRSQMAERAKSM"
     gene            complement(1608..>2226)
                     /locus_tag="PKI33_11235"
     CDS             complement(1608..>2226)
                     /locus_tag="PKI33_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37631.1"
                     /translation="HGVTFAESSVLGVLSGGISLVLYALTYAWVATRFPWYVAFVGSF
                     IVFSLSTLFLQNITVSLWFIVPVVVAAILIGLTCMPKGEVEPGESKLSQWDIPVRILI
                     GTSFILLLTGIAPFIGSRLTGLLTTIPLYVTILSIFAHRDYGPAAAAHVLRGLLYGMF
                     AFTGFFFILSLLIEETSLGVSFGLAVLTALAIQGTSLLILRRSHQ"
BASE COUNT          577 a          491 c          539 g          619 t
ORIGIN      
        1 cgttgatcga cgaatcgact ttgcgaaaac atgccgccga tgcgtttgag tttcactttg
       61 tgaagcgacc tcaaattgag tcgcgagtgc gcattgacga agggcaggtg gtgagcagcc
      121 ttagcccact ggatttgctt gaccaatatt ttgatgcggc aaaagtttcg aatgcaaatg
      181 aattgaaaaa cctggcgcag gaaatcattt cagaagactc acaagactct tgacacttct
      241 acacggacac ctgatacttg ggcatcccaa cgtcatgagg tttttatgtc caaggaacat
      301 tcacggtatc gctggtttgt ggtcgctgtg ttcttctttt tcatgctgtt gcaccagacc
      361 gataagttaa tgatcggttc actacaggtg cagatcagca atgacttcaa tatcaacaac
      421 aaacaatggg ggttggttaa ttctggagca ttgatcgttg ccacgatctt gtacccgatc
      481 tggggctatt tgtatgaccg atattcacgc acaaaactgc tttcgttagc atcttttatt
      541 tggggttcta ctacatggtt gaacgccatc atgaagagct ttggcggatt ccttgttacg
      601 cgtgcctcca ccggcattga tgactcatca tacccgggct tgtataccct aatcgctgat
      661 tatttcggac ccactcaacg cggaaagatc ttcggtattt tgcagttggc tcaacccatt
      721 ggctatttac tgggtatgat ccttgccttg atggtggctc ctgcgattgg tgggtggcga
      781 tctgttttct atatcacagg ctcgttggga ttggtgattg ccttattgat cttctttggc
      841 gtgcgtgaaa tgccgcgggg taaagccgaa cctgaatttg agaatatgac ggaaatgcaa
      901 accttcaagt tctcatgggc agaagcgcga gaaatcttca agaagaagac catgtggttt
      961 gttttcttgc agggctttgc tggtgtgttc ccgtggaatg tgattacatt cttcttcttt
     1021 gcatatcttg aaaaagaacg tggttacgat gctaatagca ttttatttac catggcgcct
     1081 gtcatcttga ttcttgccag cggatacttt gtgggcggtg ctttgggtga ttatgctttc
     1141 aagcgttcaa ataagggacg tattattgtc tcctctgttg gtgtgttgct tggagcgttg
     1201 ttcatgtacc ttgcaatcac cacaccatta gaagccaaga atcaattttt tgtctttatg
     1261 tgccttaccg ccatctttat gccgttgtct tctaccaatg tgacggcaac agtgtatgat
     1321 gtgacggttc ctgaagtgcg gtcaacggcg caggcggttg aatactttat tgaaaatgcc
     1381 ggggctgcgt ttgctcccat cattaccggc gtgattgccg atatgtatga ccttaagacc
     1441 gctattctct tgatctgtac tgtggcttgg ggcttgtgct tcttgttcta tctcggcgcg
     1501 atcttcacca ttgacaaaga cgcgaatgac ctgcgtagcc aaatggcaga acgcgcaaaa
     1561 tctatgtaaa acgaaaacgg gcttggtgaa agaaccaagc ccgtttttta ttgatgcgac
     1621 cttcgtaata tcagcagcga ggttccttgg attgctagtg cagttagaac agctaaacca
     1681 aatgaaaccc ctaggcttgt ctcttcaata agcaagctga gaataaagaa gaaaccagtg
     1741 aatgcaaaca tgccataaag caacccgcgc aagacgtgag ccgcagcggc gggtccgtaa
     1801 tcacgatggg caaaaatgct taggatggtg acataaagtg ggatggtggt gaggagcccg
     1861 gtcaggcggg aaccgataaa tggggcaata ccggtgagta gcaaaataaa gcttgtgcca
     1921 atcagaattc gcacgggaat atcccactgg ctcaatttgc tttctcccgg ctctacttct
     1981 cctttaggca tgcaagttaa tccaattaag atggctgcta caaccacagg cacaatgaac
     2041 caaagagaaa cagttatgtt ttgtaaaaat aaagtactca acgaaaaaac aatgaagctt
     2101 cctacaaacg cgacatacca agggaaacgt gtggcaaccc atgcatatgt tagggcatac
     2161 aacaccagcg aaattccgcc actgagaaca cctaaaaccg aactttctgc aaaggtcacg
     2221 ccatgg
//
LOCUS       DAOREF010000553         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37621_length_2221_cov_3.728994, whole genome shotgun sequence.
ACCESSION   DAOREF010000553 DAOREF010000000
VERSION     DAOREF010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37621_length_2221_cov_3.728994"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..753)
                     /locus_tag="PKI33_11240"
     CDS             complement(40..753)
                     /locus_tag="PKI33_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM37632.1"
                     /translation="MRSIESFWIGLHNMEIIKIKLSVSNAYLVKDRKSILVDTGSSHE
                     ADKILAEVKRAGVNEKDISLILHTHGHVDHAGSTAELKRRLGVPVAVHAEDAFMLKTG
                     TNGTVNPINFSAAIYKQLLVKPFEPSQPDLEIDKEMTLKEFGVNARVVFTPGHTKGSL
                     SILLGNKEAIIGDVMMGGFMGGNLFPARPMVHYFFENFSEVRSSIKKVIEYKPSKLYV
                     GHGGPLSLDAVISRFSNQI"
     gene            complement(716..1567)
                     /locus_tag="PKI33_11245"
     CDS             complement(716..1567)
                     /locus_tag="PKI33_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM37633.1"
                     /translation="MSLAKKWMFNHPVTHRVKDGYFENDGIRQHYVTLGEGKLVVLIH
                     GIPEFWYSWRHQIDALSQQYQVVAIDQRGFNESGKPASTDGYRVERLVEDVAALIRYL
                     GRKQAVIVGHDAGAWLAWHFAALHPELTERLVILSVPHPNAMREELAEHGEQHRASEY
                     ARTMQKEGAKPMFRTWQISLLLDRLAFPLYLSADRGTDADAITSFYQFNYPQEPYTLD
                     ESLPIIEAPTLVIHGKRDRFLIASGHEHNARWLKHEPKTVMLDADHFVHQEKPDEVNR
                     IILDWLA"
     gene            complement(1564..2004)
                     /locus_tag="PKI33_11250"
     CDS             complement(1564..2004)
                     /locus_tag="PKI33_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37634.1"
                     /translation="MYTVSITRLRVRSIFFMPLFTYHAMRTSMQVQKSEGIVGADTRF
                     EKNNVVWTKTVWTEESAMKKYRGSGAHQIAMRILSEMCSEASVTRWQQESTELPTWEE
                     AHRRMLTEGKQSKVKHPSALQASGKTAPDTMQSGFKMPTPPVTQ"
BASE COUNT          574 a          567 c          485 g          595 t
ORIGIN      
        1 cagaaatgtg catatcgatc tgaattttgt tcttatcctt tagatttgat ttgaaaaacg
       61 actgataacg gcatccagac ttaaaggtcc gccatgccca acatataact tcgatggttt
      121 gtattcaatg actttcttga tactcgatct aacttcacta aaattctcaa agaaatagtg
      181 aaccattggg cgggcaggga aaagattgcc acccataaaa cctcccatca tcacatcgcc
      241 aataatggct tccttatttc ctaagaggat cgagagcgaa cctttggtgt gacccggcgt
      301 gaaaactact cttgcattca caccgaattc cttcaacgtc atttctttat caatttcgag
      361 gtctggctga ctaggttcga atggtttaac caataattgt ttatagatgg ctgcgctaaa
      421 attaattgga ttgactgttc cgtttgtacc agtcttcaac atgaacgcat cctctgcatg
      481 gactgcaacc ggcacgccca gcctgcgctt gagttccgcc gtcgaaccgg catgatctac
      541 atgcccatgg gtatgcaaaa taagggaaat atctttttcg ttcacacctg cacgcttaac
      601 ttcagcaagt atcttatctg cttcatggct ggaaccagta tcgacaagga tcgattttct
      661 atccttaacc agataagcat ttgacacact aagctttatc ttgatgattt ccatgttatg
      721 caagccaatc caaaatgatt ctattgacct catcaggctt ttcctgatgc acaaaatgat
      781 ctgcatccaa catcacggtc tttggttcgt gcttgagcca gcgtgcatta tgttcgtgcc
      841 ctgaagcgat caagaaacgg tcacgtttcc cgtgaatgac caaggtcggt gcttcgataa
      901 tcggcaggct ttcatccagc gtatacggct cttgcggata attgaattga tagaacgaag
      961 taatcgcgtc ggcatccgtt ccacgatcag cagacaaata cagggggaac gcgagccggt
     1021 ctaacagcaa gctgatctgc caggtgcgaa acatcggctt tgcaccttcc ttttgcattg
     1081 tgcgggcata ttcactggca cggtgctgct caccatgctc cgccaattct tcacgcatcg
     1141 cattcggatg gggaacgctc aagatgacaa gccgctccgt cagttcggga tgaagcgctg
     1201 cgaaatgcca tgccagccac gcccccgcat catgaccaac gatcaccgcc tgcttccgtc
     1261 cgagataccg aatcaacgct gcaacatctt ccaccaacct ctccacccga tagccgtctg
     1321 tggaagcagg tttacccgac tcgttgaatc cgcgttggtc gattgcgaca acctgatact
     1381 gctgcgacaa cgcatctatc tgatgccgcc atgaatacca aaactcaggg atgccgtgaa
     1441 tgagcaccac caacttgcct tcacctaatg tgacataatg ctgacgaata ccatcgttct
     1501 caaagtatcc gtccttcacg cgatgagtga cagggtgatt gaacatccat ttttttgcaa
     1561 gactcattgg gtcaccggcg gagtaggcat tttgaagccc gattgcatgg tgtctggagc
     1621 ggtcttcccg gaagcctgca acgctgacgg atgtttcacc ttagactgtt taccctcggt
     1681 caacatgcgg cgatgcgctt cttcccatgt gggcagttcc gtgctttctt gctgccagcg
     1741 ggtcactgaa gcttcagaac acatttcaga cagaatacgc atggcgatct gatgcgcgcc
     1801 tgaaccacga tattttttca tcgctgattc ttccgtccag acggtctttg tccaaacaac
     1861 gttatttttt tcaaagcgag tatctgcgcc gacaatccct tcagattttt gtacctgcat
     1921 gctggtacgc atggcatgat atgtaaatag cggcataaaa aagatcgagc ggactcgcaa
     1981 gcgggtaatg gaaactgtat acatgatgac tcctatattt atttggttgt gtaggtaagg
     2041 cgatattttt gaatattacg gtttgataaa ttgcttaatg atggatttca gttcgtacat
     2101 gtaaagcgag tctccatcca caccaaaggg cggcatgttg ttcgcgatct ccaaagaaac
     2161 caagccgtga acacgactcc acaccaacat agagacagcc gacgaaaaga tgtcgtaatc
     2221 c
//
LOCUS       DAOREF010000554         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37663_length_2220_cov_3.515935, whole genome shotgun sequence.
ACCESSION   DAOREF010000554 DAOREF010000000
VERSION     DAOREF010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37663_length_2220_cov_3.515935"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKI33_11255"
     CDS             complement(<1..195)
                     /locus_tag="PKI33_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37635.1"
                     /translation="MQQLLSQLRTLPSYQQVLSELTARKPVSGLGLPRAARLPLLAAL
                     HADINQPILLITDRADHALSM"
     gene            complement(210..788)
                     /gene="pth"
                     /locus_tag="PKI33_11260"
     CDS             complement(210..788)
                     /gene="pth"
                     /locus_tag="PKI33_11260"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNM37636.1"
                     /translation="MTETFLIIGLGNPGREYKDTRHNIGFMMIDHLAETIGARGMKVQ
                     SKAIVISGLHQERKLILAKPQTYMNLSGQSVQGLLHFYKIPLENLIIAHDDMDIPFGT
                     IRIRPTGGPGGQRGMANTIELLGTKDFPRLRLGIGRPPGRMDPKDYVLQDFSKDELKL
                     MPELLTRASEAALEFVTKGLNAAMNKYNGSVE"
     gene            complement(803..1432)
                     /locus_tag="PKI33_11265"
     CDS             complement(803..1432)
                     /locus_tag="PKI33_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37637.1"
                     /translation="MLNLPIAMLKLFLILFLIFQGNTPSITSPQTGETLRGQVQIQGL
                     MDIPNFASAELAFTFDASASDPAASWFPIQTFPQPIQNPTLATWDTTAVTDGEYALRL
                     RIFLLDGTSQDVIVNTLKIRNNEPLPTPTITPTEAVFEFQPLDQTPGVSNEQNTPTPV
                     VLYSTATPLPANPASLTTSSIVTIFWQSAVLVVILFVFFSLLLRLRKNT"
     gene            1582..1956
                     /locus_tag="PKI33_11270"
     CDS             1582..1956
                     /locus_tag="PKI33_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNM37638.1"
                     /translation="MAPSRAEQLVERLRNMQAAAPDIEASAIVSVDGLIMASALQQGV
                     EEDRVSAMSAAMLSLGERISGELGRGGLEQVYIKGDKGSIVLTAIGDEAVLTAMARQE
                     AKLGMVFLEMRRAAEDLVKLVG"
     gene            2102..>2220
                     /locus_tag="PKI33_11275"
     CDS             2102..>2220
                     /locus_tag="PKI33_11275"
                     /inference="COORDINATES: protein motif:HMM:NF018158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37639.1"
                     /translation="MATKYLFFTGGVVSSVGKGVTAAATGLLLKERGFKVAVQ"
BASE COUNT          590 a          456 c          630 g          544 t
ORIGIN      
        1 catcgacaag gcatggtcgg cacgatcggt gatcagcaag atgggctgat taatatccgc
       61 gtgaagagca gctaataatg gcaaacgtgc cgcacgtggc aagcccaatc cagagacagg
      121 tttgcgtgcg gttagctcgc tcagcacttg ctgatacgaa gggagagtgc gaagttgaga
      181 aagaagctgt tgcatggact gtaaaaaatt tattctaccg agccgttgta tttattcatc
      241 gccgcgttca aacccttggt tacaaattca agggccgcct ctgacgcacg agtgagaagc
      301 tcgggcatga gtttcagctc gtctttagag aaatcttgca aaacgtaatc cttcgggtcc
      361 atacgcccgg gcgggcgacc aatgccaagc cgcaagcgtg gaaagtcctt agtgccaagt
      421 aattcaatgg tatttgccat gccacgctga ccaccgggtc cgccggtggg acgaatgcga
      481 atggtgccga atggaatgtc catatcgtcg tgcgcaatga tcaggttctc aagcggaatt
      541 ttatagaaat gcaaaagtcc ctgcaccgat tgtcctgaaa gattcatgta ggtctgaggc
      601 ttggcgagga tcaatttgcg ttcctgatgc aaaccagaaa tgacgatggc tttggattga
      661 accttcattc cgcgcgcgcc aattgtctcg gcaagatggt cgatcatcat aaagccgata
      721 ttgtggcggg tgtctttata ttcacgcccc gggtttccga gaccaataat gagaaaagtt
      781 tctgtcataa gaaaaatcca agctaggtgt tttttcgaag tcgaagaagg agggagaaaa
      841 aaacaaacag gatcacaacc aatacggcgc tttgccaaaa aatagtaacg atggatgaag
      901 ttgtcaacga ggctgggtta gcgggcaaag gagttgcagt ggaatataaa acgactggtg
      961 ttggggtatt ttgttcattg gatactccag gagtttggtc gagcggttga aattcaaaga
     1021 cggcttctgt tggggtaatg gtcggtgtgg gaagcggttc attattgcga attttcaggg
     1081 tgttgacaat gacatcttga gaggtaccgt ctaggaggaa gatgcgtaat cgcaaagcat
     1141 attccccatc ggtgacggcg gttgtatccc aagtggcgag ggtgggattc tgaatcggct
     1201 ggggaaaggt ttggatggga aaccagcttg cggcggggtc tgaggcggaa gcgtcaaacg
     1261 taaaagccaa ctctgccgag gcaaagttgg gaatatccat cagcccttga atttggactt
     1321 gtccacgtag ggtttcgccc gtttgtgggg atgtgattga aggtgtattt ccctgaaaaa
     1381 tgaggaaaag aatcaaaaac agcttgagca tggcaatcgg taagtttaac atgaagagaa
     1441 ggcaaggtca aattttgcct atttttctgt aattgtgaga ttgaaaatga attgaaatgt
     1501 gggggcaatt ctcgaatgaa gatttttctg gtaaactcta gccgttcata gaacgtaaac
     1561 gtttattagg agatctaagt tatggcacca tcccgcgctg aacaactcgt ggaacgactt
     1621 cgcaacatgc aagccgccgc cccagacatt gaagcttctg ccattgtttc ggtggatggc
     1681 ttaatcatgg catctgccct gcaacagggt gtggaagaag atcgtgtatc cgcgatgtcg
     1741 gcggcaatgt tgagccttgg cgaacgcatc tctggtgagt tgggtcgcgg tggtttggaa
     1801 caggtctata tcaaaggtga taaaggttcc atcgtattaa ccgctatcgg cgacgaagcc
     1861 gtgctcactg ctatggcacg tcaagaagcg aagctcggta tggtcttcct cgaaatgagg
     1921 cgagccgctg aagatctggt gaagttagtc ggatgagacg taaaagtcac tccacttcaa
     1981 aataaggtaa ccccgaatgg ggagggcata acgccctccc cattctcttt gtatataccc
     2041 cctcattcgc ctttcctgtc tgtgcaacag ggtgggctgt aaaaccaagg agaagaaaag
     2101 gatggcaaca aagtatttat tttttacggg cggtgtggtt tcgtcggtgg gcaaaggcgt
     2161 gactgcggca gcaaccggtt tgctgcttaa ggaacgcggc ttcaaagtgg cggttcaaaa
//
LOCUS       DAOREF010000555         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37790_length_2216_cov_4.891717, whole genome shotgun sequence.
ACCESSION   DAOREF010000555 DAOREF010000000
VERSION     DAOREF010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37790_length_2216_cov_4.891717"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="chvE"
                     /locus_tag="PKI33_11280"
     CDS             complement(<1..1061)
                     /gene="chvE"
                     /locus_tag="PKI33_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNM37640.1"
                     /translation="MEEHITYWRMVCLFQTGQATMLNDPGPVPHLFFTPIKTHPIKEK
                     KMSKKLLYNLLAVLVLLSLVLAGCGGGSSAGGDQKLVGISMPTKTSTRWISDGESMVK
                     SFEELGYATDLQFADNDIPNQIAQIENMVTKGADVLVIAAIDGSTLSDVLAKAKEAGV
                     LVIAYDRLISKTENVDYYATFDNFGVGVIQGTQLLNGLGLTPGEAAPAGVGPFNVELF
                     GGSPDDTNAFYFYDGAMSVIQPFIDSGTVVIPSGQMGMDVVGTLRWDGAVAQSRMENL
                     LSANYTDKRVDGVLSPYDGLSRGIIAALRGVGYGTADQPWPVITGQDAEVASVKAMIA
                     GEQTYTVFKDTRELAAQTA"
     gene            complement(1162..>2216)
                     /locus_tag="PKI33_11285"
     CDS             complement(1162..>2216)
                     /locus_tag="PKI33_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNM37641.1"
                     /translation="LPLTLPDQKRRVTIKDVAYAAGVSTQTVSRVINNVSYVSEGTRK
                     RVENVVEKMGYRPSTLARSLIQKRSYTLGVVTFGLKYIGPSRTLNGIADQADKLGYML
                     LMKELDNFDKHSLNEVMDSLLARQVDGIIWVAPEIGDNHAFVDENADGGMPVPILFLA
                     MRPRAEIDSVATDNYQGGVMATKHLLEIGRKRIGHISGPMTWWEAEERKRGWRDTLEN
                     AGLQVSEKFCAEGNWSSASGEQAFHQLLENYPDMDAVFVGNDQMALSAMREAHRLGIR
                     IPEQLAFIGFDNLPEAAYFYPSLTTVSQDLQLLGEESVQRIVEMIQARQNNQVLSAEA
                     RFIQPTLIVRESTQMV"
BASE COUNT          545 a          548 c          563 g          560 t
ORIGIN      
        1 ttggcggtct gggcagcgag ttcgcgggtg tctttgaaca cggtgtaggt ctgttcgccg
       61 gcgatcatag ccttgacgga agcaacttca gcatcctgac cggtgataac aggccaaggc
      121 tggtcagcgg tgccgtaacc aacgccgcgg agagcagcga tgataccgcg ggaaagacca
      181 tcgtacgggg agagcacacc atcaacgcgc ttgtcggtgt agttggcgct caggaggttt
      241 tccatgcggg attgagcaac agcgccgtcc cagcgaaggg taccgacgac atccatgccc
      301 atttgtccac tggggatcac aacggtgccg gagtcgatga agggctgaat gacagacata
      361 gcgccatcat agaagtagaa ggcattggtg tcgtcagggg agccaccgaa gagttcaacg
      421 ttgaaaggac caacgccagc aggagcagct tcaccggggg tgagaccaag tccgttgagg
      481 agctgggttc cctggatcac gccgacgccg aagttatcga aggtcgcgta gtaatcaaca
      541 ttttcggtct tggagatcaa gcggtcataa gcgatcacga gcacgccagc ttccttcgcc
      601 ttggcaagca cgtcagagag ggttgaaccg tcaatggcgg cgatcacgag cacatcagcg
      661 cccttggtga ccatgttttc gatctgggcg atctggttcg ggatgtcatt gtcagcgaac
      721 tgcagatcgg tggcgtagcc gagttcttcg aaggacttga ccatgctttc gccgtcagag
      781 atccagcggg tagaggtctt ggtgggcatg gagatgccga ccaacttctg gtcgccacct
      841 gcgctgctgc cgccgccgca gccagcgagc acaaggctga gcagaacgag aactgctaac
      901 aggttgtaaa gcaatttctt agacattttt ttctccttga ttggatgtgt ttttattggc
      961 gtaaaaaata aatgggggac aggtcctggg tcgttcagca tggtggcttg tcctgtctgg
     1021 aagaggcaca ccatacgcca atacgtgatg tgttcttcca tatgctctaa gtatgtaact
     1081 ttcttgcaat tgcacctcct tcttttttcc gtgctcttcg cgttcaacaa agaatttcag
     1141 aatagtgaag gacggaaacg ctcagaccat ctgcgtactt tcacggacga tcagagttgg
     1201 ttgaataaac cttgcttcag ccgataacac ctgattgttc tgccttgcct ggatcatttc
     1261 aacaattctt tgcactgatt cttcaccaag caattgcaaa tcttgtgaaa ccgttgttag
     1321 agaagggtaa aaatatgccg cttctggtaa gttgtcgaaa ccgataaaag ccaattgttc
     1381 agggatgcga attcctaacc gatgagcttc gcgcatggca cttaatgcca tttgatcatt
     1441 tccaacaaag accgcatcca tgtcgggata gttctctaga agttgatgaa aggcttgttc
     1501 gccacttgca gaagaccaat ttccttctgc gcaaaacttt tcagaaactt gcaaaccggc
     1561 attttcaagg gtgtctctcc agccgcgctt tcgttcttct gcttcccacc aagtcatggg
     1621 accagaaata tgaccgatgc gttttctgcc gatttccagc aggtgcttgg tcgccatcac
     1681 gccgccttga taattatcag ttgcaacgct gtcgatttct gcacgaggtc tcatggctaa
     1741 gaaaaggatg ggaactggca tgccaccatc agcattctca tctacaaaag catggttatc
     1801 gccaatttca ggtgcaaccc aaataattcc atcgacttga cgcgcgagca acgagtccat
     1861 cacttcattg agactatgct tgtcgaaatt atcaagttct ttcatcaaca gcatataccc
     1921 taatttgtct gcctggtctg caattccatt aagagtgcga gatggaccaa tgtatttcaa
     1981 tccaaatgta actactccca aggtatagct tcgtttttgg ataaggctac gagcaagcgt
     2041 actggggcgg tagcccatct tttcaactac attttccact cgttttcgag tcccctctga
     2101 aacatacgaa acattattga taacacgcga aacggtctgt gtagatacgc cagcagcata
     2161 tgctacatcc tttattgtga cacgcctctt ctgatcgggt agggtcaagg gtaata
//
LOCUS       DAOREF010000556         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37858_length_2214_cov_4.800834, whole genome shotgun sequence.
ACCESSION   DAOREF010000556 DAOREF010000000
VERSION     DAOREF010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37858_length_2214_cov_4.800834"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKI33_11290"
     CDS             <1..964
                     /locus_tag="PKI33_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37642.1"
                     /translation="MGLGLLATEMGKAVSAIHPDYNGDFTLGGGVTGGATTTFIWNGI
                     HWTSEFVFARFSLIVIALVLTFLGALFFDRFDPSRTKPRKMKGGASISEPTPASTSQA
                     LPTPHLTPLTSSPLGNKSANRFSFFTVLTAELKLLLKGQRWWWYAVAGGLIIASVVNS
                     PAITREIVLPIAWVWPILIWSAIGNREIHHNVQQLTFSSASPVWRQVPAQWLAGFIVT
                     LLMASGAVLRFGIDGDTIGLIALLSGALFIPSLALAAGVWSGTSKLFEILYMVIWYLG
                     PLNKLTALDYIGSHSDGSPQFFIPFSLVLIVLAFWGRSRQLRTV"
     gene            complement(988..>2214)
                     /locus_tag="PKI33_11295"
     CDS             complement(988..>2214)
                     /locus_tag="PKI33_11295"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNM37643.1"
                     /translation="AGSRLCNALFRLWQNRGEILREGLDWCKKFLYLSHDKQISPRAW
                     VLIHATRLASVQSPTWTAPECQAWLEESLNLARKLGDEVCIVTALEELGFIEYIGTRN
                     FDKARDLLGQSLTLSRQINYDLGASYALDWLGRTAIEQEDFPTGLILLKESLSILIRF
                     GDLRRWGLVLNQIGAIHEIQGNWNEAQSFYEQALSVAQEAQSHLHISLGLSSLGSLAV
                     GIGDFNRAITLLDQARELVQGTPNDKFLPMALSNIGEVARFQGNFDQAAAFYNESLSL
                     PQEDVQKDLVYCIQAFNESLRGEILKAKQLFATGLRTIPTGYEYVVPSVIPFIAYFMI
                     NQKVGNKAVSLLGWVDGCIKAKKYYQQPAYQAEFDRYLEQVRNELSEVEFNTAWAKGQ
                     SMSQEQVLALAMEVLQ"
BASE COUNT          537 a          572 c          495 g          610 t
ORIGIN      
        1 catggggctg ggtttattgg cgaccgaaat gggcaaggca gtcagcgcga tccatcccga
       61 ttacaacggc gactttaccc tcggcggcgg cgttacagga ggggcaacca ccacattcat
      121 ttggaacggc attcactgga cttccgaatt tgttttcgcg cgcttttcgc tgattgtcat
      181 tgccctcgtg ctgacatttc taggtgcgct tttctttgac cgattcgacc cctcgcgcac
      241 aaagccaaga aagatgaagg gcggcgcttc catttctgaa ccgacgcctg cttcaacatc
      301 tcaggcattg cccacgcccc atctgactcc acttacatcg tccccattgg ggaacaagtc
      361 tgccaatcga tttagtttct ttaccgtcct cactgctgaa ttgaaactac tgctcaaagg
      421 tcaacgctgg tggtggtacg ccgttgcagg cggtctcatc attgcctctg ttgtgaattc
      481 gcctgcgatt acgcgtgaga tcgtgcttcc cattgcatgg gtctggccca tcctgatctg
      541 gtctgccatc ggcaaccgcg aaattcacca caacgttcag caattgactt tctcctctgc
      601 ttcgcctgtg tggcgtcaag tccctgcaca atggctggct ggatttatcg tcaccttgct
      661 catggcaagc ggcgcggttt tacgttttgg catagacggc gacacgatag gactaatcgc
      721 tttactctca ggcgcgctct tcatcccatc ccttgcgctt gccgcagggg tgtggagcgg
      781 aaccagcaaa ctctttgaga tcctttatat ggtcatctgg tatcttggtc cgctcaacaa
      841 acttacagcg ctcgattaca tcggctcgca cagcgacggg tctccccagt tcttcatccc
      901 attttcactg gtattgatcg ttttggcttt ttggggaagg tcgagacagt tgagaaccgt
      961 ataaatatca cctcacagta cgcaccgcta ttgcaatacc tccatcgcca gcgcaaggac
     1021 ctgctcctga ctcatagact gccctttcgc ccaagcggtg ttgaactcaa cttcggagag
     1081 ttcattgcga acctgttcca ggtaacgatc gaattctgct tgataggcag gttgttggta
     1141 atactttttt gctttgatac atccatcaac ccatccaagc aaggatactg ctttgttgcc
     1201 aactttctga ttaatcataa aataagcaat aaatggaatt actgaaggga caacatattc
     1261 atatccagtt gggatagttc gcaagccagt tgcaaagagt tgcttcgctt ttaatatctc
     1321 accacgcagg ctttcattaa atgcctgaat acaatacact aggtcttttt ggacatcctc
     1381 ttgtgggagc gataacgact cattataaaa ggcagccgct tgatcaaaat ttccttgaaa
     1441 gcgagcaacc tcaccgatgt tagaaagcgc cataggaagg aacttgtcat taggcgtgcc
     1501 ttgtacaagt tcacgggctt gatccagcaa tgtaatagct cgattgaaat ctcctatccc
     1561 taccgccaga gatccaaggg aggatagccc cagagagata tgtagatgtg attgtgcttc
     1621 ctgtgcaact gacagggctt gttcataaaa actctgggct tcattccagt tgccttgaat
     1681 ttcatgaatg gccccaattt ggttaagtac caatccccat cttctaagat caccaaagcg
     1741 gatgagaatc gacaggcttt cttttaaaag tataagtccg gtgggaaagt cttcttgttc
     1801 tatagctgtt cttccaagcc agtcaagagc gtagctagca ccaaggtcat agtttatctg
     1861 gcgggataat gtcagactct gcccaagtaa atcgcgggct ttatcaaaat ttcttgtccc
     1921 aatgtattca atgaaaccaa gttcttccaa ggcagtcacg atgcaaacct cgtctcccaa
     1981 tttacgagcc aaatttaaac tttcctcaag ccatgcctga cattctgggg cagtccaagt
     2041 tggggattgg acactcgcta atcttgttgc atggatcaac acccatgcgc ggggtgaaat
     2101 ttgcttatca tgggaaagat aaagaaattt tttacaccag tctaaacctt ctcttaaaat
     2161 ttctccgcga ttttgccaca atcgaaacag tgcattgcag aggcgcgagc ctgc
//
LOCUS       DAOREF010000557         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_37886_length_2213_cov_5.367933, whole genome shotgun sequence.
ACCESSION   DAOREF010000557 DAOREF010000000
VERSION     DAOREF010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37886_length_2213_cov_5.367933"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..334
                     /locus_tag="PKI33_11300"
     CDS             140..334
                     /locus_tag="PKI33_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNM37644.1"
                     /translation="MNVQRQALRRSRHDRIIAGVCGGLGEFFGISPFWFRLAMFIAFI
                     PGGVPGILIYFIAMLIIPSE"
     gene            complement(389..952)
                     /locus_tag="PKI33_11305"
     CDS             complement(389..952)
                     /locus_tag="PKI33_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37645.1"
                     /translation="MQYKKLLQIFVLLAVVVSAFGFTGGASAWYCTSYVTVQWGDTLS
                     GIASLCGTTVDAIRAANPGLGWWVYAGQVLYIPTGSTPSYPAPSYGGTYVVQWGDTMA
                     KIAGYYGVTVNDLLAANPQVWSPSLIYAGQVLNIPAAPAYHTVSYGETLRMIAGYYGT
                     TVYSLQLLNPTIYDPNYIYPGQSIRVR"
     gene            complement(1116..1424)
                     /locus_tag="PKI33_11310"
     CDS             complement(1116..1424)
                     /locus_tag="PKI33_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37646.1"
                     /translation="MTNQWYSLGLGDGMLAAAPSEEIQVYFAKVFSAMGMPPEMAVFT
                     RHESEGRLHCEVVAYFSPKASEVAQAFDAEPCPKPVRAGLGLLAGSETAWSNLFDEAQ
                     "
     gene            1598..2059
                     /locus_tag="PKI33_11315"
     CDS             1598..2059
                     /locus_tag="PKI33_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37647.1"
                     /translation="MPFAYRLDKNIKIFFLVTYGHCTFSDIFSPSLDPETKPENRPRV
                     KFILDNMLGDLEIDMEGMKFFIKAMNELKQTGFQLEPTAFITNKRGLEIFIKSLDLLV
                     DNDVTTHMAFSSLDEALIWLDEVENAAAIKQINDELLESLRLQYGPHPQLV"
BASE COUNT          635 a          538 c          467 g          573 t
ORIGIN      
        1 ttcctgcgtt gaattttgaa gcggggcatt atacactgga taattttttc aatttcaagc
       61 cagtctggac aactactgta attttatctg aaaccatagg gaaaattgtg cgtatatctc
      121 tacataaaaa ggagaaaaaa tgaacgtaca aagacaggct ttaagacgct caagacatga
      181 ccgcatcatc gccggggtat gtggcgggct tggcgaattc tttggcatta gccccttctg
      241 gtttcgcctc gccatgttta tcgctttcat tccaggtggc gtgcccggga tattgatcta
      301 ctttattgcg atgcttatca tcccatctga ataatgtagt ttaaacaaaa agaagtccgc
      361 accatagcgg acttcttttt gtttgttttt atcgtactcg aatggactga ccggggtaaa
      421 tataattcgg gtcgtagatc gtggggttca gtaattgcag gctgtaaacc gttgtgccgt
      481 aatatccggc aatcatacgc aatgtctcac cataggagac agtatggtaa gcaggtgccg
      541 ccgggatgtt aagcacttgc ccagcataga tgaggcttgg gctccaaact tgcgggttgg
      601 cagccagcaa gtcgttgacg gtcacgccat aataaccagc aatctttgcc atagtgtcac
      661 cccattgcac tacataggtt ccaccatacg aaggcgcagg gtacgatggt gtagacccgg
      721 ttgggatgta gaggacctgc ccggcgtaca cccaccagcc caaaccaggg ttggcagcgc
      781 gaattgcatc caccgtggtg ccgcagagag aggcaattcc gctcagggtg tcaccccact
      841 gcacagtcac atacgaagtg caataccaag cagaggcacc gccggtaaag ccgaaagcag
      901 aaacaacaac tgccagcaaa acaaatattt gtaaaagttt cttatattgc atatcatcct
      961 ccattgatgg ttgaaaacaa aaacgactct tcttaaatta tatagattcc acgcgcgaat
     1021 tcaatcacac gaatgtactt atgaatctaa taacgctaaa gagtcctctt ttgttcctct
     1081 agagattccc tctgcaatga aattagaatg gagttttatt gcgcttcgtc aaacaaattg
     1141 gaccaagccg tctcactgcc tgccaagagt cccagcccgg ctcttactgg ttttgggcag
     1201 ggttcggcat cgaatgcctg agccacctct gaggcttttg gcgaaaaata agccaccact
     1261 tcacaatgca accgtccttc agattcgtgg cgagtaaaaa ccgccatttc aggcggcatt
     1321 cccatcgccg aaaaaacttt cgcaaaataa acttgaattt cttctgatgg agcggctgcc
     1381 aacatgccgt cgcctaaccc aagggaatac cattgatttg tcatattgga gttttccatt
     1441 ttgaatactg gcacaaataa ttcagactaa aaatgtcgca ttgatatgta agtctaacac
     1501 tctacgagag aatattacaa aataaattat catcagcgcc tgttatactt gaaccaagtt
     1561 atcaatcaac acctcaacct cgaagtggac atttcttatg ccctttgcat atcgccttga
     1621 taaaaatatc aaaattttct tccttgtcac ctatggtcac tgtacattca gcgacatctt
     1681 cagcccatca ctagatccag aaaccaaacc cgaaaatcgt cccagagtca aattcattct
     1741 cgataatatg ctaggcgacc ttgaaataga catggaaggc atgaaatttt tcatcaaggc
     1801 catgaacgag ttaaagcaga cgggctttca actggaaccc accgccttca taactaataa
     1861 aagagggctc gaaatattta ttaaatccct tgatttactt gtagataatg atgtaacaac
     1921 ccatatggca ttctcttcac tcgatgaagc gctgatctgg ctggacgaag ttgaaaacgc
     1981 cgcagctatt aagcaaatta acgacgaact attggaatcc ttacgtttgc aatatggtcc
     2041 tcatccgcaa ttggtgtaaa agctaaactg cccataccct cgggtatctg cctttacagc
     2101 gacctgcgcc accacgctcg caaacgcaac cgtaggggcg actcgccatg gactgactcg
     2161 cgccttgtct ggcaagactt ccttgcttga cgtaacaacc gagggcattg aag
//
LOCUS       DAOREF010000558         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38123_length_2206_cov_4.731288, whole genome shotgun sequence.
ACCESSION   DAOREF010000558 DAOREF010000000
VERSION     DAOREF010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38123_length_2206_cov_4.731288"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKI33_11320"
     CDS             <1..955
                     /locus_tag="PKI33_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNM37648.1"
                     /translation="TAIFHEMLTYHMGWTGEGAGSQATGKRIRPLITLLSVASINNKE
                     EKKDDGTYWLHAKSMAAAVELVHNFSLVHDDIQDNSELRRGRKTVWTKWGPPMAINVG
                     DALFVIANQAVVDLKEHYPAEMVVRAAEILNECCLDLTRGQYLDMSYEERNDLSMDDY
                     WPMIGGKTSALLSACTQVGALLGYANEEKIELYRQFGYNLGLAFQVQDDILGIWGDEA
                     VTGKSAASDLVEGKNSLPVLYALEKNGEFAKRWKQGPIKQDEVSQVAALLEKEGGKAY
                     AEKMSEELTQKALEYLRQADPQGEAGEAILGLANMLLKRKQ"
     gene            1033..1103
                     /locus_tag="PKI33_11325"
     tRNA            1033..1103
                     /locus_tag="PKI33_11325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1065..1067,aa:Gly,seq:ccc)
     gene            1244..1636
                     /locus_tag="PKI33_11330"
     CDS             1244..1636
                     /locus_tag="PKI33_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37649.1"
                     /translation="MPENGRGTILYVEDNPDNRNLIRRVLNAEGYAVVEAAQATQAFA
                     KLESEHIDLILMDINMPDMDGYTLTAKIKKMDKYKNIPIVAVTANVMRGDREKSLEAG
                     CDGYIQKPIDIDTLAQQIERFMTRRTNV"
     gene            1629..2087
                     /locus_tag="PKI33_11335"
     CDS             1629..2087
                     /locus_tag="PKI33_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37650.1"
                     /translation="MSDQDQTPAVSDTQPIRKPTIPKDAAVLVVEDNVSNFVLIARML
                     GYLGIHCEWKTSGYEVVEYADTLPRLDLILMDIRLPYEDGYGALKKIRASERFKSVPI
                     IAVTAEASQEQMKKAKESGFDGFLGKPLDPDRFPDQIRRILNGEPVWEFS"
BASE COUNT          617 a          462 c          566 g          561 t
ORIGIN      
        1 cactgccatc ttccatgaga tgttgaccta tcacatgggt tggacaggtg agggcgctgg
       61 ttcacaagca acagggaagc gcatccgtcc gttgattact cttctttcag tagcttctat
      121 taataataaa gaggaaaaga aagatgatgg cacttattgg ctacacgcaa aatcaatggc
      181 agctgcagta gaacttgtcc ataatttctc tttggtgcat gatgatattc aagacaactc
      241 agaactccgt cgcggacgga aaacggtgtg gacgaaatgg ggtccgccca tggcaattaa
      301 tgttggagac gcattgttcg taattgccaa ccaagctgta gtcgatctta aagaacatta
      361 tccagctgag atggtagttc gtgctgcgga gattttgaat gaatgttgtt tagatctaac
      421 acgtggtcag tacttggata tgtcttatga agaacgcaac gatctttcga tggatgatta
      481 ctggcccatg attggaggaa agacctctgc cctactctct gcctgcaccc aggttggagc
      541 tcttcttggc tacgcgaatg aagaaaaaat agagctatac cgtcaatttg gctataactt
      601 aggactcgca ttccaggttc aagatgatat tctcggcatt tggggcgacg aagctgtgac
      661 cgggaaatct gccgctagcg acttagtgga aggaaaaaat tcgctacctg ttttgtatgc
      721 gcttgaaaag aatggtgaat ttgccaaacg ctggaaacaa ggtccgatta agcaggatga
      781 agtgagccaa gtagctgctt tactagaaaa agaaggcggc aaagcgtatg cagagaaaat
      841 gtctgaagag ttgacgcaga aagcacttga gtatttgcgt caggcagacc cacaaggtga
      901 agcaggcgaa gctattcttg ggttggcaaa catgctcctg aaacgcaaac aatagtgagc
      961 gtggaatgaa gcggcgcgtc gtcaggcttg acataggttt tgagcgtgac gtataatcgc
     1021 gccgttgttc atgcgggtgt agttcagtgg tagaacatgt gcttcccaag cacaatacgc
     1081 gggttcgatt cccgccaccc gctcaaagaa gccgaggaga ccttccctcg gcttttcgtt
     1141 atgctatact agccgtacct ttattatttg taagttgtaa ggtagctgtc atgaggggtg
     1201 ccttctatat taaagtaaaa ttctgcctgg agaaatacca gctatgccag agaatggacg
     1261 cggaacgatc ttgtatgttg aagataatcc tgataaccgc aatctgattc gcagagtgtt
     1321 aaatgctgaa ggatatgcgg ttgtggaagc cgctcaagcg acacaggcgt ttgccaagct
     1381 tgaaagcgaa catattgatt tgatcttgat ggatatcaac atgcccgata tggatgggta
     1441 tacattgacc gcaaagatca agaagatgga taagtataaa aatattccga tcgtagctgt
     1501 cactgctaat gtgatgcgcg gcgataggga aaaatctctt gaggcaggat gcgacggtta
     1561 tattcaaaaa ccgatcgaca ttgatacgct tgcacagcaa atcgaacgct tcatgacaag
     1621 gagaaccaat gtctgaccaa gaccaaaccc ctgctgtttc tgatactcag cccattcgca
     1681 aaccgacgat tccgaaggat gcggcagtat tggtagtgga agataatgtt tcaaatttcg
     1741 ttttgatcgc ccgtatgtta ggctatttgg gcattcactg tgaatggaag acttcggggt
     1801 atgaagttgt tgagtatgcc gacaccctcc cccgtttgga cctgatcctt atggatattc
     1861 gccttccata tgaagacggg tatggcgcac tgaaaaagat ccgcgcttcg gaacgtttca
     1921 agtctgttcc gatcattgca gtgacggctg aagccagcca ggaacaaatg aagaaggcga
     1981 aagaatctgg tttcgatggc tttttgggga agccgcttga ccccgaccgt ttccctgacc
     2041 aaatccgccg gatcttgaac ggagaacctg tctgggaatt cagttagtgt tgttgtaaaa
     2101 tttactaaat gacaatccct gcagcgccga ctacgaatgg aaaacaacaa ggtagggtca
     2161 agggcagcct gaccagcact ctcgttagaa cgctcttgat ctttac
//
LOCUS       DAOREF010000559         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38124_length_2206_cov_3.947466, whole genome shotgun sequence.
ACCESSION   DAOREF010000559 DAOREF010000000
VERSION     DAOREF010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38124_length_2206_cov_3.947466"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKI33_11340"
     CDS             complement(<1..455)
                     /locus_tag="PKI33_11340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM37651.1"
                     /translation="MIYNSKIQRLNYNPIFGQRRIPPMLSTPSFDEAGMQYQQNRITH
                     WNKIAQKRDSWKGLGGWYHRRLEEVYKFLVAPNQRILEIGCGQGELLASLNPLRGVGV
                     DFSEEMIVRAKQRYPNLEFHQMDAHDLSHLEGKFDIIILSDTTNDLWDV"
     gene            454..930
                     /locus_tag="PKI33_11345"
     CDS             454..930
                     /locus_tag="PKI33_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNM37652.1"
                     /translation="MTVESTNEEMRELTLLEQIESDPDVNQATLAQQLGVAVGTVNWH
                     LKRLIAKGAVKVARAERKKLRYIITPEGLALRARLAVNYVENSFSVYRRTRQKVKDNL
                     AKVQKAGYDQIRIIGSGDVADICKLTCIEQGITVVTDKSAPALVLDGYRIRLEGLE"
     gene            927..1937
                     /locus_tag="PKI33_11350"
     CDS             927..1937
                     /locus_tag="PKI33_11350"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM37653.1"
                     /translation="MTDRHILVTGGAGYIGSLLTSELLRQNYRVTLLDSLLFGGESIL
                     PFMSHPNFHFLKTDVTEPRAIRDAVKRDWGKPDAVVHLAGIVGFPACQAVGKQVAWKY
                     NVEATKMVYGQSADLGVERFVFASTYSNYGYSEDGKPVTEETPLHPQSLYAETKIAAE
                     EYLLSQKDSACAPLLFRFATLYGISPRTRFDLIVNQFVLEAFTKRQLIIYQRGYSRSF
                     VHIRDVVRGIIMGLEAEQNKIRGQVFNLGSDTGNYSKNDIVGFVLKRMPETIVEYKNL
                     TFGGDMRDVTVSFEKIRNVLGFETTLNVDDGVREVLYALKSGLIKDPTDDRYRNAQFI
                     VQ"
     gene            1954..>2206
                     /locus_tag="PKI33_11355"
     CDS             1954..>2206
                     /locus_tag="PKI33_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM37654.1"
                     /translation="MAENFWSNKRVVVTGGGGFLGSFVVEKLKERGATDIFIPRKKDY
                     DLTQREAIERLYVDILKSVDPKNVVIIHLAANVGGIGANR"
BASE COUNT          589 a          491 c          539 g          587 t
ORIGIN      
        1 tgaacatccc ataaatcatt tgtagtatct gaaagaataa tgatgtcgaa cttgccttca
       61 aggtgagaaa ggtcgtgtgc atccatctga tgaaattcaa gatttgggta acgttgctta
      121 gcacgtacaa tcatttcttc agaaaagtca acgcctacac ctcttaacgg gtttaaggat
      181 gcaagtaatt caccttgtcc acagcctatt tctaggatgc gctggtttgg agcaactaga
      241 aatttatata cttcttcaag gcggcggtga taccagccac ctaaaccttt ccaagagtcg
      301 cgtttttggg cgatcttgtt ccagtgagta attcggtttt gctggtattg cattccagct
      361 tcatcaaaag atggggttga tagcattggt gggattcgtc tctgtccaaa aattgggtta
      421 taattcagtc gctgaatttt ggaattataa atcatgactg ttgaatctac aaatgaagaa
      481 atgcgagaac tcacccttct cgaacaaatc gaaagtgacc ccgatgtcaa ccaagctacc
      541 cttgcgcaac aattgggagt agccgttggc acggttaatt ggcacttaaa gcgcctcatt
      601 gcaaaagggg cagttaaagt agctcgcgcc gagcgtaaaa aactccgtta tattatcacg
      661 cctgaagggc ttgcgttgcg cgcccgcctt gcagtgaatt atgtcgagaa ttctttttcg
      721 gtatatcgcc gcacccgcca aaaggtgaag gataatctcg ctaaagttca aaaagcgggc
      781 tacgaccaaa tccggatcat cggctcgggc gacgtggcag atatctgtaa attaacctgc
      841 attgagcaag gtattacggt tgtcactgat aaatcggcac cggctcttgt gttggatggc
      901 taccggatca gactggaagg cttggaatga cggatcgtca tatcctggtt acgggaggcg
      961 cggggtatat cggctctctc ttgacctcgg aactgctccg ccaaaattat cgggtcacac
     1021 tccttgactc tttgctcttt ggcggcgaaa gtattttgcc gttcatgagc cacccgaatt
     1081 ttcattttct taagaccgat gtgaccgagc ctcgtgccat tcgagacgct gtaaaacgtg
     1141 actggggcaa gccagatgct gttgttcacc tagcagggat tgttggcttc ccggcatgtc
     1201 aggcggtggg caagcaggtg gcgtggaagt ataacgttga ggcgacgaaa atggtctatg
     1261 ggcagtcagc agacctgggc gtggaaagat tcgtgttcgc atccacctat agtaactatg
     1321 gttactcaga agatggcaag cccgttaccg aagagactcc gcttcatcct caatcacttt
     1381 acgccgaaac gaaaatcgct gctgaagagt atttgctttc ccaaaaagat tctgcttgtg
     1441 cacccttgct ctttcgcttc gcaacattgt atggaatttc accccgcacc cgctttgatt
     1501 taatcgttaa tcaatttgta cttgaggcgt tcaccaaacg ccaactcatc atctaccaac
     1561 gcggatactc ccgctcattt gtgcatatcc gcgatgtggt gcgtggcatc attatgggac
     1621 tcgaagcaga gcaaaacaag attcgcggac aggtcttcaa ccttggctca gatactggaa
     1681 attattccaa aaatgacatt gtaggctttg tgctaaaacg gatgcctgaa acgattgttg
     1741 aatataaaaa cctgaccttc ggtggcgata tgcgtgatgt gaccgtttcg tttgagaaga
     1801 ttcgcaatgt gcttggcttt gaaaccacct tgaatgtaga tgatggtgtg cgcgaagtgt
     1861 tatacgcttt gaaatctggg ttgatcaaag acccaaccga tgaccgatac agaaatgcac
     1921 aattcattgt gcaataatgg agagatataa aacatggcag aaaatttctg gagtaataaa
     1981 cgtgtagttg tcacaggcgg aggcggtttc ctcggctcgt tcgtagtgga gaaattgaag
     2041 gagcgcggcg cgacggacat ttttattccg cgcaaaaaag actacgatct aacccaacgc
     2101 gaagccatcg aacgcttata tgtggatatc ctcaaaagcg tagacccaaa aaatgtagtc
     2161 attattcacc ttgctgcgaa cgtaggcggt atcggtgcga atcgtg
//
LOCUS       DAOREF010000560         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38130_length_2206_cov_3.204091, whole genome shotgun sequence.
ACCESSION   DAOREF010000560 DAOREF010000000
VERSION     DAOREF010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38130_length_2206_cov_3.204091"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKI33_11360"
     CDS             complement(<1..432)
                     /locus_tag="PKI33_11360"
                     /inference="COORDINATES: protein motif:HMM:NF002696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HNM37655.1"
                     /translation="MEDILNTLLQQEQELQFAHFNESTAWQIGTQMVERAMRENLPVT
                     IDITRGQHQLFHCSMPGTAADNDEWVKRKVRLVNRFGHSSFYMGQKLKQKGKTIEESY
                     LISESEFAAHGGCFPIIIKGTGMVGTITVSGLPQEEDHKFVV"
     gene            complement(593..1747)
                     /gene="gguB"
                     /locus_tag="PKI33_11365"
     CDS             complement(593..1747)
                     /gene="gguB"
                     /locus_tag="PKI33_11365"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM37656.1"
                     /translation="MSNLFKNNIREYGMLIALIVIMIFFYFMTDGILMKPINITNLVL
                     QNSYVIIMALGMLLIIVSGWIDLSVGSVAAFIGSIAGVMIVKWEWNWILVMGICLTIG
                     GVIGAWQGYWVAYLKIPAFIVTLAGMLIFRGLTLVMVRGEAIGPFPEGFRNISGGYIP
                     DFFHYEGFHLTTIVVGVLVSILLIFSDIRTRRNQQKYGFEVTPTYFFIVKQILITAAV
                     MGFCYLMASYKGIPNLLVIMFPLVTIYSFLTSQTVIGRRIYAMGGNEKAARLSGVNTQ
                     RLLFLTFVNMGVLAALAGMVVASRLNSSTPNAGEGFELDVIAACFIGGASASGGIGTV
                     IGAVVGAFLVGVLNNGMSILGVGIDWQKVVKGMVLLLAVLFDVYNKNKSS"
     gene            complement(1753..>2206)
                     /locus_tag="PKI33_11370"
     CDS             complement(1753..>2206)
                     /locus_tag="PKI33_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM37657.1"
                     /translation="NITLSNLNAISDAGVINEPKELTVTREYRDKLNIKCSSILQLAL
                     NLSGGNQQKVVLSKWLFANPEILILDEPTRGIDVGAKYEIYTIINQLASEGKAIILIS
                     SELPEILGVCDRIYVMREGKIVGEMPAKEASQELVMKHIMFEQEAIAQ"
BASE COUNT          600 a          519 c          507 g          580 t
ORIGIN      
        1 gactacaaac ttgtggtctt cttcctgtgg caagccggag acggtaattg tcccgaccat
       61 gcctgtccct ttgatgatga tggggaagca gccgccatgc gcggcgaatt cactttcaga
      121 gatgagatag ctttcttcga tggtcttgcc tttttgcttt aatttctgcc ccatgtaaaa
      181 cgaactatga ccaaagcgat tcaccagtcg caccttgcgc ttgacccact cgtcattgtc
      241 cgcagcggtg cctggcatac tgcaatggaa gagttggtgt tgtccgcgtg tgatgtcgat
      301 ggtgacgggg aggttttcac gcatggcacg ttccaccatt tgagtgccga tctgccatgc
      361 ggtggattca ttgaagtgcg cgaactgaag ttcctgttct tgttgcaata aagtgtttag
      421 tatgtcttcc atctgcctgt cctttgggaa cgcaatacct acgaaaacca aattcgcttt
      481 aagtcaatct tttaaatata gtttttaata tttacagagg caatactgat gagatctttg
      541 ttgttgagaa cgagagcagt cgttgaaact gctctcgttg gtggaccaat tattacgaac
      601 tcttgttttt gttgtacaca tcaaacaaaa cggctaaaag aagtaccatg ccttttacga
      661 ccttttgcca gtcaatacct acgccaagaa tggacatgcc attgttcaac acaccgacaa
      721 gaaaggctcc aactactgca ccaataaccg taccaatacc gccactcgca gaagcaccac
      781 cgatgaagca cgcggcgatt acgtcaagct caaagccttc acctgcattt ggtgtagaac
      841 tgttcaagcg ggaggcgaca accatccccg ccaatgctgc aagaacgccc atgtttacaa
      901 aggttaggaa caaaaggcgc tgtgtgttga caccggaaag acgggctgcc ttctcatttc
      961 cacccatggc atagatacga cgcccaatca cagtttgact ggtgaggaag gagtaaatcg
     1021 tcaccagcgg gaacatgatt acaagcaagt tggggatgcc tttataagaa gccatcaagt
     1081 agcaaaagcc catcactgca gctgtaatca agatctgttt cactatgaag aagtatgtgg
     1141 gagtgacttc aaatccgtat ttttgctgat tgcgtcgcgt tcgtatgtca gaaaagatca
     1201 agaggatgga tactagaacg cctactacaa tggttgtaag gtgaaaccct tcatagtgga
     1261 aaaaatctgg aatatagccg ccactaatgt tgcggaagcc ttccgggaac ggtccaatcg
     1321 cttcgccgcg aaccatgaca agggtaaggc cgcggaagat caacatgcca gccagcgtca
     1381 cgatgaatgc aggaattttc agatacgcca cccaatatcc ctgccaggcg ccaattacac
     1441 cgccgatcgt aagacaaatc cccattacca atatccaatt ccattcccat tttacgatca
     1501 ttacaccggc tatggagccg atgaaggcag ctactgaacc gaccgagaga tcgatccatc
     1561 ctgaaacaat gatcaacagc atgcccagtg ccatgatgat cacgtagctg ttttggagga
     1621 cgagattggt gatgttgatc ggtttcatca gaatgccatc tgtcatgaaa tagaagaaaa
     1681 tcatgatcac gatcaacgca atcaacatgc cgtactcgcg gatattgttc ttgaaaaggt
     1741 tggacaaagt tttcattgtg cgatagcctc ttgttcaaac atgatgtgct tcattacaag
     1801 ttcttgcgac gcttccttgg cgggcatttc accgacgatc ttgccttcgc gcataacata
     1861 aattcggtca caaaccccta atatttcggg taactcggag gagattaaaa taatcgcctt
     1921 tccttcactg gcaagttgat tgatgatcgt gtaaatttcg tatttggcgc ccacgtcaat
     1981 gccgcgggtg ggctcatcca aaataagaat ttcagggttg gcaaaaagcc acttgcttag
     2041 tactactttt tgttgattgc cgccagagag gttcagcgcc aattgaagga tactggaaca
     2101 tttaatgttt aatttatctc gatattcgcg ggttacggtc aattcctttg gctcgttaat
     2161 gacaccagcg tctgagatcg cgttgaggtt ggataatgta atgtta
//
LOCUS       DAOREF010000561         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38217_length_2203_cov_3.540503, whole genome shotgun sequence.
ACCESSION   DAOREF010000561 DAOREF010000000
VERSION     DAOREF010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38217_length_2203_cov_3.540503"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PKI33_11375"
     CDS             <1..986
                     /locus_tag="PKI33_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNM37658.1"
                     /translation="GETLIFSDVRNDSRAPESLGEIQAASGRWALVMIPLMVRNQRIG
                     LVILSHPGVHEWADEELSPYRAVAAQLAIAIDHRSQQTLLQQRGQQLAVLQERQRLAR
                     ELHDSVTQLIFSTTLIAQSISPAWKRNPEEGEKRVERLLELSQTALREMRSLLFELKS
                     SEEIQDTELPVTLTGLERIRHYGLLGAIRLLAQDFSADGILVSVDVDEASEKLFRKQT
                     GSRSGKEPMVDESIYRITQEALNNAVKHAKARQIFISISCQGRQLWVSIADDGKGFKS
                     TSIEKQKPEDGSGLGMKTMKERAEALGGKLSVTSAPKQGTTIELTIPLKES"
     gene            989..1630
                     /locus_tag="PKI33_11380"
     CDS             989..1630
                     /locus_tag="PKI33_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37659.1"
                     /translation="MKAIRVLIVDDHEIVREGLQTLLSEEKEIEVVGTAGDSDEAVSM
                     VKRHKPDVVLMDVILPGVDGIETTRRVVATNPSTRVLMLTSYSDDQRVREAIQAGAIG
                     YLLKDVLKADLLNAIRSAAAGKPALHAEAQQFLMRQLTGSDSAPHTRLTAREFNILQL
                     IAAGKSNKEIALALHLTEGTVKGYVSTIFDKLEVADRTQAALYAVEHGLLKPK"
     gene            1792..>2203
                     /locus_tag="PKI33_11385"
     CDS             1792..>2203
                     /locus_tag="PKI33_11385"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNM37660.1"
                     /translation="MPSKHSNFIFRVAIVLMLVFISSCGGAPSDSETAPESSSGVIAK
                     NASYSSPIAISDDDALVVVANTLNSTVSLINVAGDANTKLTEVQVGEEPRSVVITPNK
                     AFAYVTNQVSGTVSVIDLNSQTKVTDITVGAEPYG"
BASE COUNT          577 a          461 c          613 g          552 t
ORIGIN      
        1 cgggagagac tcttatcttc tcagatgtca gaaatgactc gcgagcgcca gagtcacttg
       61 gtgaaattca ggcagcatct ggcagatggg cattggtgat gattccgcta atggtgcgaa
      121 atcaacggat tggattggta attttgagtc accctggggt gcatgaatgg gctgacgagg
      181 aattaagccc gtatcgggca gtggcggcac aattggcaat tgcaattgac catcgctctc
      241 aacagacctt gttacaacaa cgcggacagc aactggctgt gctacaggaa cgtcagcggc
      301 tggctcgtga actgcacgat tctgtgactc agttgatctt tagcacgact cttatcgctc
      361 agtcaatttc gccggcgtgg aagcggaacc cagaggaagg cgaaaagcga gtagaacgct
      421 tgttggagtt gagccagact gcgttgcgtg agatgcgttc gctactgttt gaattgaaat
      481 caagtgaaga gattcaagat actgagctgc ccgtcacatt gaccgggctg gagcgtattc
      541 gccattatgg gctattaggt gccattcgat tgttggcaca agatttttca gcagatggta
      601 ttcttgtaag tgtggatgtg gatgaggcga gtgaaaagct ttttaggaag cagacaggtt
      661 caaggtcggg gaaagagccg atggtggatg aaagcattta ccgcatcact caagaggcgc
      721 taaacaatgc cgtcaagcat gccaaggctc gtcagatttt tatatcaatc tcttgccaag
      781 gcaggcaatt gtgggtttct attgcagatg atggaaaagg cttcaaaagt acatccattg
      841 agaaacaaaa gcccgaggat ggtagcgggt tgggaatgaa gacgatgaaa gagcgggcag
      901 aagcgctggg tggcaaactc agtgtgacat ctgccccgaa acaaggtacg accattgaat
      961 tgaccatacc tcttaaggag agttaaagat gaaagctatt cgtgtcttga ttgtggatga
     1021 ccatgaaatt gtccgcgagg gattgcagac tttgctaagc gaagaaaaag aaatcgaagt
     1081 ggtgggcact gcgggggata gtgatgaagc cgtctctatg gtgaagcggc ataaaccgga
     1141 tgtggttctt atggacgtga tcttgccagg tgtggatggt attgagacaa cccggcgagt
     1201 cgttgccact aacccatcta ctcgggtgtt gatgctaaca tcgtattctg acgatcagcg
     1261 agtgcgagaa gccattcaag caggcgcgat tggttattta ttgaaagatg tgctcaaggc
     1321 agatttatta aatgcgattc gatctgccgc tgccggaaaa cctgccttac acgcagaagc
     1381 gcagcaattt ctgatgcgcc aactcaccgg ttcagattct gcgccgcata caaggttgac
     1441 cgccagagag ttcaatatct tgcaattgat cgccgccggg aaaagcaaca aagagatcgc
     1501 gctcgctttg cacctcacag aagggactgt gaaaggctat gtcagtacga ttttcgataa
     1561 attggaagta gcagaccgaa cacaagccgc gctttatgcg gtggaacatg ggctattgaa
     1621 gccgaaataa actgccttcc tccgtctgat tctgtaccta actttggtta gccgtaatgg
     1681 ctaacctttt tcttttgatt caatggctct tgatggtttg ttttgaaagg gctttcttct
     1741 atgattatga gtatcccgtt atcggtatcc attatataga aggagagaag tatgccatcg
     1801 aaacattcaa attttatttt tcgagtcgca attgtgctta tgcttgtatt cataagttcg
     1861 tgcggcgggg cgccgtctga ttctgagact gcgccagagt caagcagtgg ggtgattgca
     1921 aagaatgcga gctattccag cccgatcgcc attagtgatg atgatgcgtt ggtcgtggtt
     1981 gcgaatacgc ttaacagcac ggtgtcgcta atcaatgtag caggcgatgc caacaccaag
     2041 ttgaccgagg ttcaagtagg cgaagagccg cgctctgtgg tgatcacgcc gaacaaagcc
     2101 tttgcttatg tgaccaatca ggtcagcggt acggtctcgg tcatcgactt gaattcacag
     2161 accaaggtca ctgatatcac cgttggagct gagccgtacg gga
//
LOCUS       DAOREF010000562         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38238_length_2202_cov_4.074988, whole genome shotgun sequence.
ACCESSION   DAOREF010000562 DAOREF010000000
VERSION     DAOREF010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38238_length_2202_cov_4.074988"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKI33_11390"
     CDS             complement(<1..734)
                     /locus_tag="PKI33_11390"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNM37661.1"
                     /translation="MSKNNNPLNLRLILVLGTAALLLIVQVLGLFSTITTPIERIEYS
                     MQDTFVRLRGAEKPSGEIVIVAIDDQSFNWTGLQWPWPRSYFAEIVDQINKGGGKVVG
                     MDLILFEPDEKQPENDLVLAKALGESPAAVAVMQINKTGGEGYATETLSQPLSAYRDV
                     LDGIGTTDVKRDEDAIVRNVSAYSIYQGQVYPHWAFELAKLYLNAEQPIFGNSSVKFN
                     GQNIPLRSNELPIYFAGPAGTYPTYSA"
     gene            complement(762..1967)
                     /locus_tag="PKI33_11395"
     CDS             complement(762..1967)
                     /locus_tag="PKI33_11395"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNM37662.1"
                     /translation="MKRYLVVLSILVIIGVILTGCQTKEETSPLSAGLSELTGLVEMK
                     QAGQDAFTQAAADAVLEVNGQIQTGDDGRVRLDLSSGTIIRVAPSSFFTLTSNDEVEG
                     GLLTKIKLEAGKIFIVLNGGQTDVETPSGVASVRGSYLKVEVDPVTKDIHITCLEGTC
                     SATNPNGEQIIFTNGQSVILFHQESDGSWKSPLLGDMTMEDFEEWLQNSNDSETKKYY
                     DEGVAKLLEATASPTEPPTEVPTDVPPTEVSSVNGQGETSNACSQLQEPIDGRALNNA
                     GKVNFAWSEQPNAQTYIITFIKEDGSTARIETSSNSAEFYIEVLPAGGNYQWFVTAYG
                     ADGKEICKSNSANFSKPQAEPTEKPKATKDPNAEEPASGTAGCPCDPYDYLNYSDCVG
                     SHMDVCSIP"
     gene            complement(1992..>2202)
                     /locus_tag="PKI33_11400"
     CDS             complement(1992..>2202)
                     /locus_tag="PKI33_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37663.1"
                     /translation="KEVVIQVSCDAASITVELVQEGATNNDQVECGKPKIIKVTSWQE
                     YLIKVTSKPVSESLVEYKLNIKIIK"
BASE COUNT          557 a          484 c          472 g          689 t
ORIGIN      
        1 gcagaatagg ttggataggt tccggcaggt cctgcaaaat agataggaag ttcattggac
       61 cgtaacggaa tgttttgacc gttaaattta acgcttgaat ttccaaatat tggttgttca
      121 gcattcaaat ataactttgc aagttcaaat gcccaatgcg gataaacctg tccctgatag
      181 atactgtatg ccgatacatt tcgcacgatg gcgtcttcat cgcgttttac atctgttgtg
      241 cctatgccat ccagaacatc acggtacgca gataatggct gagacagggt ttcggttgca
      301 tagccttctc ctcccgtttt attgatctgc atcacagcaa ctgcggcggg gctctctccg
      361 agtgcttttg cgagaactag gtcattttcg ggttgcttct catcgggctc gaaaaggatt
      421 aaatccatac caacgacctt gccgccgcct ttattgattt ggtcaacgat ctctgcaaaa
      481 taggagcgag gccaaggcca ttgcaagccg gtccagttaa aagattggtc gtcaattgct
      541 acaatgacaa tctcaccaga tggtttttcc gctccacgca ggcgcacaaa tgtatcctgc
      601 atggagtatt caatccgctc aatgggtgtg gtgatggtgc taaataatcc cagaacttga
      661 acaattaaga gtagtgcggc tgtcccaagt accagaataa ggcgtaagtt taagggatta
      721 ttattttttg acatgtctta tcctttgatg gcttgagatg attaaggaat actacaaaca
      781 tccatgtgag agccaacaca atcggaatag ttcaagtaat cataggggtc acaaggacag
      841 ccagcggtac cactagcagg ctcttctgca tttggatctt tggttgcttt tggcttttca
      901 gttggttctg cttgcggttt tgagaaattg gcactattgg atttacaaat ttcttttccg
      961 tctgcgccgt aggctgtgac aaaccattga tagtttccac cagcaggcaa aacctcaatg
     1021 taaaattctg cgctatttga actggtttca attcgagcgg tgctgccatc ttcttttata
     1081 aatgtaataa tgtaggtttg cgcgttcggt tgttcactcc atgcaaaatt gacttttcca
     1141 gcattattga gcgcacggcc atctattggc tcttgtaact gcgaacacgc attgcttgtt
     1201 tcaccttgtc cattgacaga actgacttcg gtaggcggta catcggttgg aacttcagtt
     1261 ggcggttccg taggagatgc agtggcttct aaaagctttg ctactccttc atcatagtat
     1321 ttctttgttt cgctatcatt actgttctgg agccattctt caaagtcttc cattgtcata
     1381 tccccaagta atggagactt ccatgaacca tcggattctt gatggaaaag aattacagat
     1441 tgcccattgg taaaaatgat ctgttctcca tttgggttag tagcagaaca agttccttcg
     1501 agacaagtaa tatgaatatc tttagtgaca gggtccactt ctacttttaa atatgagccg
     1561 cgcactgaag ctacaccaga gggtgtttcc acgtcagttt ggccaccgtt gaggacgatg
     1621 aaaattttcc ctgcttcgag ctttattttg gttaacagac cgccttctac ttcgtcattt
     1681 gaggttaggg tgaaaaatga agatggcgcc acacgaataa tcgttccaga agaaagatca
     1741 agtcgtacgc gcccatcgtc tccagtttga atctgcccat tcacttcaag tacggcatcc
     1801 gctgcggctt gagtaaaggc atcttgccca gcttgcttca tttccacaag tcccgttaat
     1861 tcactaagcc cagcagaaag aggggatgtt tcctctttgg tctggcatcc cgtaagaatc
     1921 acccctatga tgacgagtat tgataaaacc acaagatatc ttttcatttc tctctccgtt
     1981 cgtttggtgc tttatttgat aatttttatg ttcaatttgt attcgactag gctttcactg
     2041 acaggcttcg atgtaacttt aatgagatat tcctgccatg aagtgacttt aatgatttta
     2101 ggctttccac attctacttg gtcattatta gttgcacctt cttgaactaa ttctactgta
     2161 atgctagcgg catcgcatga aacttgaatt acaacttctt tt
//
LOCUS       DAOREF010000563         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38380_length_2197_cov_4.236695, whole genome shotgun sequence.
ACCESSION   DAOREF010000563 DAOREF010000000
VERSION     DAOREF010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38380_length_2197_cov_4.236695"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PKI33_11405"
     CDS             complement(<1..908)
                     /locus_tag="PKI33_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNM37664.1"
                     /translation="MSNEYDLKSVKLPYLAGGVLKLFASLVEGPLQSALIPSLFESAG
                     LTWLRKQQIEEAPTYLPIHYVGELQTKDAAIPEAQWPVTPSQTRGFQFASIFDFAKAY
                     RAGTTTPEDVAKKVLDAIEASDKSDAPLRAFIAVNRDEVMRQARESTERFKAGKPLSI
                     FDGVPVAIKDEMDVTPYPTTVGTSFLGKSPAKEDSEVAARLRKAGAMLVGKTNMHEIG
                     INVFGLNPHHGTTRNPYNTGHFTGGSSSGSATAVAAGLVPVAIGADGGGSIRIPAAFC
                     GLVGLKATFGRVSEYGAFPLDWSVAH"
     gene            complement(1046..1588)
                     /locus_tag="PKI33_11410"
     CDS             complement(1046..1588)
                     /locus_tag="PKI33_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37665.1"
                     /translation="MSSTIALLMFIALIGFIAFLFWRQNRIFNDIQAFYKDNKMVYQE
                     TAPVEHPFIYTSIKPICSKGNLKPDMPFTFMLGSRSEGAGQSRVLHTYIGVFIPDHPR
                     LTDAWLNTWKHKVAERGDNWAQHSDVEAHEKSWGLMGAPEDLPIVAKRINNGVLIAWS
                     GLHIRKVFEARLDELKQTLS"
     gene            complement(1612..1944)
                     /locus_tag="PKI33_11415"
     CDS             complement(1612..1944)
                     /locus_tag="PKI33_11415"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNM37666.1"
                     /translation="MAYDLKLAERIRAEVKGLPIIEKKMFGGVGYMLHGNMACGILED
                     NLIVRVALNDYEKTLKRKHVKIFTMKNGPRPMKGWVMVEPEGYKTSKQLKDWVKTGID
                     VAINLPPK"
     gene            complement(1964..>2197)
                     /gene="upp"
                     /locus_tag="PKI33_11420"
     CDS             complement(1964..>2197)
                     /gene="upp"
                     /locus_tag="PKI33_11420"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNM37667.1"
                     /translation="MLATGGSATATAEVLKRWGVTKIKFVGLIAAPEGIKAMQAAHPD
                     IDIYVAAIDDHLNERAYIVPGLGDAGDRQFGTG"
BASE COUNT          514 a          532 c          533 g          618 t
ORIGIN      
        1 atatgcgcca cgctccaatc caacggaaac gcaccatatt cgctcacacg cccaaatgtg
       61 gctttcaagc cgaccaaacc gcaaaacgcc gccggaatac gaatcgaccc gcctccatcg
      121 gcaccgatgg cgacgggcac cagtcctgca gcaacagcgg tggcagagcc gcttgaactt
      181 cctcctgtaa agtgaccggt gttgtatggg ttgcgggttg tgccgtgatg agggttcaac
      241 ccaaagacat tgatgccgat ttcatgcatg tttgtcttgc cgaccaacat ggcacccgct
      301 ttgcgtaaac gtgccgccac ttccgaatct tctttggctg gagatttccc taagaaggat
      361 gtgccgactg tcgttggata cggtgttaca tccatttcat ctttgatcgc aaccggcacg
      421 ccatcgaaga tgctgagcgg tttgccagcc ttgaaacgtt ccgtcgactc gcgggcttga
      481 cgcataactt catcgcggtt cacggcgatg aatgctcgta gcggcgcatc agacttgtca
      541 ctggcttcaa tggcatctaa gacctttttt gccacgtctt cgggtgttgt cgttccggca
      601 cgataggctt tggcaaaatc gaaaatgctc gcaaattgaa agccacgtgt ttgagatggt
      661 gttacgggcc actgtgcttc gggaattgcc gcgtctttgg tttgtaattc accaacatag
      721 tgaatcggca gataagtagg tgcttcttca atctgttgtt tacgtagcca agtcaacccg
      781 gcgctttcaa acaagctggg aatcaatgca ctttgcaatg gtccttccac taatgaggca
      841 aacaacttca ataccccgcc cgccagatag ggcagcttca cagatttgag atcgtattca
      901 ttgctcattg tttctcctca aggaaaatgc tgttatgtgg tgcaaatcac tgcgggtatc
      961 acttgtcagt gtgagtatca gtttgggatg ttgaacccgc ttcaaatgag tcggaagcgg
     1021 ggttgtatcc catatgattt ggttgttagg agagcgtttg ctttaattcg tctagccgtg
     1081 cttcaaatac ttttcgaata tgcagccctg accaggcaat tagaacgcca ttgttgatgc
     1141 gtttggcaac aatgggtaaa tcttcagggg ctcccatcag tccccagctt ttttcatggg
     1201 cttccacatc ggaatgttgt gcccagttat caccgcgttc tgctactttg tgtttccagg
     1261 tattgagcca agcatcggtc agccgtgggt ggtctgggat gaagacccca atgtaggtat
     1321 ggagaactcg agattgcccg gcaccttcag acctggaacc aagcatgaat gtgaaaggca
     1381 tatcaggttt taggttgccc tttgaacaga tcggttttat gctggtgtaa ataaaaggat
     1441 gctctacagg ggcagtttct tggtagacca tcttattgtc tttgtaaaaa gcttgaatgt
     1501 cgttgaagat tctattttga cgccaaaaca agaaggcgat aaaaccgatg agcgcaataa
     1561 acatcaataa agcgattgtg gaagacattc tcaatctcct tttatgtata tttacttggg
     1621 aggtaggtta atagcaacat ctatgcctgt ttttacccaa tccttaagtt gttttgatgt
     1681 tttgtatcct tctggctcca ccatgaccca gcccttcatg ggtctgggtc cgtttttcat
     1741 ggtgaatatt ttgacgtgtt tgcgtttaag agtcttttcg taatcattca gcgcaacacg
     1801 tacgattaag ttatcttcaa gaatgccgca tgccatgttg ccatgcaaca tataaccaac
     1861 gccgccaaac atctttttct cgatgatggg caagcctttc acttcggcac gaatccgttc
     1921 tgcaagtttc aggtcgtatg ccatatgtga tttggtatat cgtttatcct gttccgaact
     1981 ggcggtctcc agcatcgccc aagccgggca cgatgtacgc acgttcgttg agatgatcgt
     2041 caatggcagc aacatagata tcaatatcgg ggtgggcggc ttgcatggct ttgatgcctt
     2101 ctggtgctgc gattagcccc acaaatttga tcttggtcac accccaacgc ttcaaaactt
     2161 ccgctgtggc tgtggcagaa ccaccggtgg caagcat
//
LOCUS       DAOREF010000564         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38421_length_2196_cov_3.402616, whole genome shotgun sequence.
ACCESSION   DAOREF010000564 DAOREF010000000
VERSION     DAOREF010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38421_length_2196_cov_3.402616"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKI33_11425"
     CDS             complement(<1..286)
                     /locus_tag="PKI33_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37668.1"
                     /translation="MPIAELFSRENIHSLPFPFTEDGEYARRYLIPLMSEDPQKYIRN
                     VHNTQLMAVKVGETIIPITVTDFHPQNTYTVSPYSHYVSYGAFEEVKHLNN"
     gene            complement(277..759)
                     /locus_tag="PKI33_11430"
     CDS             complement(277..759)
                     /locus_tag="PKI33_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNM37669.1"
                     /translation="MNVKTKVTVIGVISFFALACNLGLQPATSLPEATQTSIPLDASK
                     VYIEGNLSWEEPLVNAPIDLVVIESSDPYASTVTDAEGNFSFSGLEPFSPGFGIGIDI
                     PVVEWKCQEPDLLDETWFWTASAYQYEENKVRIWLQSYEELTVPSGILIQMSIELKCP
                     "
     gene            complement(756..1595)
                     /locus_tag="PKI33_11435"
     CDS             complement(756..1595)
                     /locus_tag="PKI33_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM37670.1"
                     /translation="MKIDILHAGYCTSPEHLAIQGGRWRTIHFPAMFTLLRHPKFGAM
                     LFDTGYSYRFFEETKKFPMRFYRWMTPVHLNEEDLVVNQLATFNLRPTDISFVFISHF
                     HADHVGSLCDLPKSRYVYLPHAYSHLRSLSPSKDMKHAFLRGQIPSDFDARSQEVDMS
                     RSILLSEEYAPFKTGYDLLGDGSIIGVELPGHAHGQMGIFARNDEDKIFFFVADAAWL
                     KRSIVENRPPHKIADMLFPDPAAYRETLGKLQTYTLTHPDTVVVPSHCDETISQLSAS
                     GHK"
     gene            complement(1666..>2196)
                     /locus_tag="PKI33_11440"
     CDS             complement(1666..>2196)
                     /locus_tag="PKI33_11440"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM37671.1"
                     /translation="FQNGLAVISIRPRALFGEEDTVIFPRLLARLKSGRLPILGDGEN
                     VVDLTYIQNVVDALLLCAEAPEASLGKKYNISNGEPVKIWSLINQICDELGFSRPSRH
                     ISRQTAHMAGGTLEFLYSLIPYSPEPPLTRMSVSMLSNSTTLDISAAKAELGYQPKVS
                     VEEGVQRFLKWWKEKN"
BASE COUNT          593 a          500 c          542 g          561 t
ORIGIN      
        1 tattattgag atgctttact tcttcgaacg cgccatacga aacataatgg ctgtacggcg
       61 aaaccgtata cgtattctgc gggtgaaaat cggtgaccgt aatggggatg atcgtctcac
      121 cgactttgac cgccatcaac tgcgtattat gcacattgcg aatgtatttt tgcgggtctt
      181 cactcatcaa tgggatgaga tagcggcgcg catattcgcc atcttctgtg aatgggaacg
      241 gcagggagtg aatgttttcg cggctgaaga gctcggctat gggcatttca attcaatact
      301 catctggatc aatatgccgc ttggcactgt gagttcttca taactttgta accagatccg
      361 aactttattt tcctcatact ggtaggcgct tgctgtccaa aaccaggttt catctaacag
      421 atcaggttcc tggcacttcc actccactac gggaatatca atccctatac caaagcccgg
      481 actgaaaggc tcaagacctg aaaacgaaaa atttccctct gcatcggtca cagtggaagc
      541 atatgggtct gaactctcta tgacaaccaa gtcaatagga gcattcacca gaggctcttc
      601 ccaagaaaga ttcccctcaa tgtacacttt ggatgcatca agcggaatag acgtttgagt
      661 ggcttcgggt aaagacgttg caggttgaag acctagatta caagcaaggg cgaaaaagct
      721 tatcacgcct ataacagtta ctttagtttt gacgttcatt tatgacctga tgcactgagt
      781 tgtgatatcg tctcatcaca atgcgacggg acaaccaccg tatcgggatg ggttagtgta
      841 taggtttgca attttcccaa cgtttcacgg taagcagccg ggtcggggaa gagcatatcc
      901 gctatcttgt gtggcggacg gttctccaca atggaacgct ttaaccaagc agcatcagcc
      961 acgaaaaaga agatcttatc ttcgtcattc cgagcaaata ttcccatctg tccatgtgcg
     1021 tgaccgggca actccacgcc gatgatggag ccgtctccga gcaggtcata gccagtctta
     1081 aagggcgcat attcttctga aagcaaaatg gatctgctca tatctacttc ttgcgagcgg
     1141 gcgtcaaagt ctgaggggat ctgtccgcgc aggaaggcgt gtttcatatc tttagatggc
     1201 gataagctgc gcaaatgtga ataggcatgc ggcaaataca cataacgaga ctttggcaaa
     1261 tcacaaagcg agccaacgtg atcggcatgg aagtgagaaa taaagacgaa agatatgtcc
     1321 gtaggtcgaa ggttgaaggt tgcaagttga ttgactacga ggtcttcttc attgagatga
     1381 acaggggtca tccagcggta aaaacgcata gggaatttct tcgtctcttc gaagaagcga
     1441 taagaatagc cagtgtcgaa aagcattgcg ccgaatttgg gatgacgcaa aagagtgaac
     1501 atggcaggga aatggatcgt gcgccagcgt ccgccctgaa tggcgagatg ctcgggagag
     1561 gtacagtagc ctgcgtgaag aatgtcgatt ttcataagag ccttttaacc acaaagtaca
     1621 caaaggtcac aaagtaaact tttgtgtact ttgtgacctt tgtggttaat tcttttcttt
     1681 ccaccatttc aaaaaccgtt gcacgccttc ttccaccgaa acctttggct gatagccgag
     1741 ttctgccttc gcagcagaaa tatcgagtgt ggtgctattc gataacatac ttacagacat
     1801 gcgagtcaac ggtggctcag ggctgtaagg tattagggaa tacaagaact caagtgtgcc
     1861 acctgccatg tgggcggtct ggcgggagat atggcggctg ggacgtgaaa agccaagctc
     1921 atcacatatt tggttgatca ggctccatat tttcaccggt tcgccatttg aaatattata
     1981 ttttttaccc agagaagcct ctggtgcttc ggcacaaagc agcaatgcat ccactacatt
     2041 ttggatatag gtcaggtcaa cgacattctc gccatctcca aggatcggca gcctgccgct
     2101 tttgagtctt gccaaaagcc gtgggaagat gaccgtatct tcttctccaa aaagcgcccg
     2161 cggacgaatg gagatcacag ccagtccgtt ttggaa
//
LOCUS       DAOREF010000565         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38517_length_2193_cov_4.181010, whole genome shotgun sequence.
ACCESSION   DAOREF010000565 DAOREF010000000
VERSION     DAOREF010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38517_length_2193_cov_4.181010"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKI33_11445"
     CDS             <1..437
                     /locus_tag="PKI33_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37672.1"
                     /translation="PTQLSMPLTPATAWKKAEDTASKPKVKKEATSSADKLPVKGEVL
                     TPVVVSKEEKDKAAKVIKQVERSDAYQLYRRNGRRAQIGEFDFRNMLFATMESSPETL
                     KRNLDLFKRYASIHFRTDLIAFLEFCEENFAALMISKVKGKK"
     gene            440..>2193
                     /locus_tag="PKI33_11450"
     CDS             440..>2193
                     /locus_tag="PKI33_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM37673.1"
                     /translation="MAGSSRKLQYADRVAIQQADQAIRKDVFRALVEIITNSNDSYLR
                     LEQAGWSVSGEIYIDVLRKHKNSIIRIWDNAEGMNDSRMDKVVGTYGEATSGIKEDKN
                     VRGMWGRGLKDSIFGLGHGYVRSFKGGNFYSCSLLIKNGIPTFSLDEPTRALVPIRQQ
                     YGILEGNGTVIEIIVSRDDVKVPQFDNFRNYLQRHFELRPIMSNPKRKIILREMDSDW
                     NVKQEHVLSYKAPKGVKVLSERVKIPNFPAWVKLELYRSSIQLSMRGEEGDYADGGLL
                     VISKGMVLSLTMLKYENDPLAAYFYGSMQCDYLHDLLKNDEPVLTATRDGINWSHPFA
                     KALRQAVEEKIEPLIEKERKHALREEQTKLDKQLRQKIDKALHELNHIALNELSNRKE
                     GESRIQLPASGIMFFPERAYVQTGRTLSLILRADITKKAKPNAVVEITSNSPEIIVLT
                     KQVKLKPHKADPSVGEAIVRIEGRQVGGEGLVIARVNKMHAQAMVQVHSKKEVLVEPP
                     ARSKNMLFNDIRFDDRMDPRQRVYFDRVNSSIVIATNAPSVKMYLDENKRLDTSMQGQ
                     VLLAELVTEAVCREIAREG"
BASE COUNT          613 a          500 c          580 g          500 t
ORIGIN      
        1 agcccacaca gttgagtatg ccacttactc cggcaacagc gtggaaaaaa gctgaagata
       61 cagctagcaa gcccaaggtc aagaaagaag cgacctctag cgcagacaag ttgcccgtca
      121 aaggtgaagt gctgactccg gtggttgtat ccaaagaaga gaaggataaa gccgcgaagg
      181 tcatcaagca ggttgaaagg tcagacgcat atcagttata ccgtcgtaat ggacgacgcg
      241 ctcagatcgg tgagtttgat ttcagaaata tgttattcgc cacgatggag tcttcgcccg
      301 agaccttgaa acggaatttg gatctattca aacgatatgc cagcattcac ttccgtacgg
      361 atttgatcgc gttcctcgaa ttttgtgaag aaaattttgc agctttaatg atctcgaaag
      421 ttaaaggtaa aaaatagtta tggcaggcag tagtagaaaa ttacagtacg cagaccgcgt
      481 tgcgatccag caggctgacc aggcgattcg caaagatgtg ttccgcgcgc tggtggaaat
      541 catcaccaat agcaacgaca gttatttgcg ccttgaacaa gccggttggt ctgtaagcgg
      601 cgaaatttat attgatgtgc tgcgtaagca taagaattcc atcattcgca tttgggacaa
      661 tgccgaaggc atgaacgact cgcgcatgga taaagtagtg ggtacctacg gcgaagcgac
      721 cagcggtatt aaggaagata aaaatgtgcg cggcatgtgg ggacgcggat taaaagattc
      781 catctttggt ttggggcacg gatatgtgcg ttcattcaaa ggcggaaatt tctattcctg
      841 ttctttgctc atcaagaatg ggatcccaac cttttcgttg gatgaaccaa ctcgcgcctt
      901 agtgccgatc cgccagcagt acggaatcct tgaaggtaat gggacggtaa tcgagattat
      961 cgtttcgcgt gacgatgtaa aagtgccgca gttcgataac ttccgcaatt acctgcaacg
     1021 tcactttgag ttacgtccca tcatgagcaa ccccaagcgt aagatcatct tgcgcgaaat
     1081 ggactctgac tggaacgtca agcaagagca tgtgcttagt tacaaagcgc cgaagggtgt
     1141 gaaggttctg agcgaacgcg taaagattcc taacttccct gcctgggtga agttggagtt
     1201 gtaccgttct tccattcagc tctccatgcg cggcgaagaa ggcgattatg ccgatggcgg
     1261 tctgctcgtg atcagcaaag ggatggtctt atcgctgacc atgctcaagt acgagaatga
     1321 tccgttggcg gcatatttct atggctcgat gcaatgtgat tatctgcatg acctgttgaa
     1381 aaatgatgag ccggtcctca cggcaacgcg tgatggaatc aactggtcac atccctttgc
     1441 caaggcgctg cgacaggcag tggaagagaa gatcgagccg ctgatcgaaa aagaacgcaa
     1501 gcatgccctg cgcgaagagc aaaccaagct cgacaaacaa ctgcgccaaa agatcgataa
     1561 agcgttacat gaattgaatc atatcgcgtt gaacgagttg agcaatcgca aagaaggtga
     1621 gtctcgcatt caattgccag cttcgggcat tatgttcttc cctgaacggg cttatgtgca
     1681 aacggggcgc acgctttcgc tcattctgcg agcagatatt acgaagaaag ccaagccgaa
     1741 tgcggttgtg gagatcacat ctaatagccc cgaaattatt gtgttgacca aacaagtgaa
     1801 attgaaacct cacaaagcgg accctagcgt gggcgaagcg attgtgcgta ttgaaggtcg
     1861 ccaagtgggc ggtgaagggt tggtcatcgc aagagtcaac aagatgcatg cacaagccat
     1921 ggtgcaagtc cattcgaaga aagaagtgct cgtggagccg cccgcacgca gcaagaatat
     1981 gctcttcaac gacatccgct tcgatgatcg tatggaccca cgtcagcgtg tgtattttga
     2041 ccgtgtcaac tcaagtattg tgatcgcaac caatgccccc tctgtaaaaa tgtatctgga
     2101 tgaaaacaag cggctcgaca cgtccatgca ggggcaagtg cttctagcag aactcgtcac
     2161 tgaagccgtg tgccgcgaaa tcgcccgcga agg
//
LOCUS       DAOREF010000566         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38624_length_2190_cov_5.775176, whole genome shotgun sequence.
ACCESSION   DAOREF010000566 DAOREF010000000
VERSION     DAOREF010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38624_length_2190_cov_5.775176"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1399)
                     /locus_tag="PKI33_11455"
     CDS             complement(86..1399)
                     /locus_tag="PKI33_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNM37674.1"
                     /translation="MLRSRTFLYFLIGISLLIALVFGGYYLYNTYRVRTVVIATNEEG
                     SQSYIMMDALAKAVQQRDSRIKVKIVQTAGSADIMKLLESHQADFGTAQLDAELPDQV
                     QTVALLYPQAFHMIVPAASDIWSPADLRGKKIATSSKSGGGYKSVFELLAYYGLSPED
                     VEVIPFEDGDLRDAAFINGDVDAMFRANTIGSSSLRELAAQKDIRFLQLEQFDAMRIQ
                     YPYLFQYVIPKGTYRADAPIIPVQDTKVVGVPTLLFVNANVSEDIVHLFASALFESQN
                     DIVQSTIQGIWIESPVNYRTLVPEIHSGALSYFNSDEPGFFSKYYNELSLLFTLGPVL
                     VSLYFTLIASFQAKQKSTASSYNLELAKLLADLTYSKNVRTVQYIERSLFESLNEVVE
                     AMEEGKISTEDLNIYSLVWEKAMDAVRRRYDLLKKKPKRKKPVRK"
     gene            complement(1417..>2190)
                     /locus_tag="PKI33_11460"
     CDS             complement(1417..>2190)
                     /locus_tag="PKI33_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM37675.1"
                     /translation="FLDSDLNEFPFTVPVIRSLPEIKFTSPVTFFVGENGSGKSTVLE
                     ALACAVESITVGTESVKTDKTLAPVRKLSKYFKLAWTKRTRKGFFMRAEDFFGYARSM
                     RETREELEEEYNNVERDYEGRSKYAADLARSAYAGQLAAMKNRYGRDLDNFSHGEAFL
                     TLFQARFVPGGLYLLDEPEAALSPSRQLSFLSALRQMVEEEAQFIIATHSPIILAYPG
                     AQIFSFQNGAIKQVQYEELEHVQLTKDFLNNPQSYLRHL"
BASE COUNT          628 a          546 c          463 g          553 t
ORIGIN      
        1 aaagcccgtc cattttcgag aatggcacag gcagtttcat cacaagaagt ttcgatggca
       61 agaatacgag cagatttcaa ttcattcatt tgcgtacagg tttctttcgt tttggcttct
      121 ttttcagcaa atcatatcgt cggcgcacag cgtccatggc tttttcccaa accaacgaat
      181 agatattcaa atcttcagtg gaaatctttc cttcttccat ggcttccaca acttcattaa
      241 gcgactcgaa gagactacgc tcaatatact gcactgtgcg aacatttttt gagtatgtca
      301 ggtcagccag taatttcgcc aactccaagt tgtaactaga tgccgtggat ttctgttttg
      361 cctgaaagct cgcaattaat gtgaaataca aagagaccag aactggtccg agcgtaaaca
      421 acaagctaag ttcgttgtaa tacttcgaga aaaaaccggg ctcatcacta ttgaaatatg
      481 acagcgcacc agaatggatt tccggcacga gcgtccgata attcacagga gactcgatcc
      541 aaatcccttg aatagtgctt tgaacaatat cattctggct ttcaaacaaa gcagaggcaa
      601 ataaatgcac aatgtcttca gaaacattgg cattcacaaa caagagggtt ggcacaccaa
      661 caacttttgt atcttgaaca gggatgatgg gagcgtctgc gcgataggtt ccttttggaa
      721 ttacatactg aaacaaatac ggatattgaa tgcgcattgc atcaaactgt tccagttgta
      781 aaaaacgaat gtccttctgc gcagccaatt cgcgcagact gctgctacca atcgtatttg
      841 cacggaacat ggcatcaaca tccccattta taaaagcagc atcccgcaaa tcgccatctt
      901 caaatggaat cacctcaaca tcttcaggag ataatccata ataagccagt aactcaaaga
      961 cagatttata accgccgcca cttttagaag atgttgcgat cttttttcca cgcaaatcag
     1021 ctgggctcca gatatctgaa gccgcaggca cgatcatatg aaacgcctgc gggtacaaca
     1081 acgccacggt ttggacctga tctggtagtt cagcatccag ttgcgcggta ccaaagtctg
     1141 cttgatgtga ctcgagcaac ttcataatgt cggcagaccc ggcggtttga acgatcttca
     1201 ccttgatacg agaatcacgc tgttgaaccg ccttcgccaa agcatccatc atgatgtaac
     1261 tctggctgcc ttcttcattt gtcgcaataa ccaccgtacg aacacggtac gtgttgtaca
     1321 aataatatcc accaaacacc aatgcgatca ataaggaaat gccgatcaag aaataaagaa
     1381 aagtgcgcga gcgaagcatt gagatcattc ctgtatctat aaatgccgca agtaagattg
     1441 cgggttattc aaaaaatcct tggtaagttg gacgtgttca agctcctcgt attgtacttg
     1501 ctttatagcc ccattttgaa agctgaatat ttgcgcacct ggatacgcca aaatgatcgg
     1561 cgaatgagta gcgatgataa actgcgcctc ttcttcgacc atttgcctga gtgccgaaag
     1621 gaagctaagc tgtctggacg gcgaaagcgc agcctcgggt tcatccaaca gatataagcc
     1681 accggggaca aaacgcgcct gaaacaaagt gaggaatgcc tcgccgtgcg agaagttgtc
     1741 caagtctcta ccgtaacggt tcttcatcgc cgcaagttga ccggcatacg ccgaacgcgc
     1801 caagtctgcg gcatatttcg agcgtccttc ataatcgcgc tctacattgt tgtattcttc
     1861 ttccagttct tcacgcgtct ctcgcatgga ccgggcatat ccgaaaaaat cttcggcgcg
     1921 cataaagaag cccttgcgcg tacgtttcgt ccatgcaagt ttgaaatact ttgatagctt
     1981 acgaacaggc gcaagggttt tatcggtctt gacgctttcc gtccccaccg taatggactc
     2041 taccgcacaa gccaatgcct ctaacaccgt cgatttgccc gagccgttct cccccacaaa
     2101 aaaggttacc ggtgaggtga acttgatctc tggcaaagaa cgaatcaccg gcaccgtgaa
     2161 cggaaattca ttcaggtcac tatcgagaaa
//
LOCUS       DAOREF010000567         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38747_length_2186_cov_3.174566, whole genome shotgun sequence.
ACCESSION   DAOREF010000567 DAOREF010000000
VERSION     DAOREF010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38747_length_2186_cov_3.174566"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PKI33_11465"
     CDS             <1..1086
                     /locus_tag="PKI33_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNM37676.1"
                     /translation="TAATGVLIEKLIKSVPELAPFVRVVHGDGRVGAALVASKPDYIF
                     MTGSTATGKKIMQTAAETLTPVALELGGKDAMIVLEDAELEAAARWGAWGAFFNTGQT
                     CMSVERVYVVESKYDEFVNLAVLQAQELKSGYSTELNSPFFMGPVTDQRQVKIIDGHL
                     QDALDKGAKILTGGKRDGMFYDPIVLVNVDHSMKIMRDETFGPVMPIVKVKNEEEAIH
                     LANDNAYGLGASIWSNDISHAKRVADRVEAGSIIINDSIAQFGVPMLPFGGTKDSGFG
                     RTHGKEGLLQFTRPYSFAIGGAPVKMDVATVLREMGHYELATMIMGVLFGTTLKQKVE
                     TFLQFFRRGKKQAPPAGETSSNTAAAD"
     gene            complement(1154..>2186)
                     /locus_tag="PKI33_11470"
     CDS             complement(1154..>2186)
                     /locus_tag="PKI33_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNM37677.1"
                     /translation="SVMNHPQSVTYREYGTQAMPQMTQNFAALPTHNFSRGTFEDVEK
                     ISGETMREFILNRGKPSDPSHACMAGCTIKCSNVFGGEDGKIIVSPLEYETVGLMGTN
                     LDINSLNSIGRMNWQVNDLGLDTIEVGGALGVAAEAGIMRWGSEEDGLKLIDEIRKGT
                     ELGRVLGDGAVAVGKKYGVERVPAVKGQSMSAYEPRSIKGTGVTYATTPQGADHTCGL
                     TIRAQVNHLDPTQQKDASLNAQLNMAGYDTIGACIFAGFGYAATPDGVVKRLLKARYG
                     WEDVPDNVLQALGKETIKLEREFNKRAGFTKEDDRLPKWMMEEALPENGSVFDVSEDV
                     LDHIFDGIE"
BASE COUNT          576 a          561 c          499 g          550 t
ORIGIN      
        1 actgctgcta caggcgtatt gatcgaaaag ctcattaaga gcgtaccaga gttagcgcca
       61 tttgtacgcg ttgttcatgg tgacggcaga gtgggcgcag ccttggttgc cagcaagcca
      121 gactacattt ttatgactgg ctccacagcc accggcaaga agatcatgca aaccgcagca
      181 gagacactta cccccgtagc cctagaactt ggcgggaaag atgccatgat cgtgcttgaa
      241 gatgcggagc tagaagccgc tgctcgttgg ggagcctggg gagccttctt caataccggt
      301 cagacgtgca tgtctgtaga acgtgtctat gtggtcgagt caaaatatga cgagtttgta
      361 aatctggcag tgctgcaggc acaagagcta aagagcggtt attccaccga actcaatagc
      421 ccattcttta tgggacccgt cactgaccag cgccaagtta aaatcattga tggacatttg
      481 caagatgcgt tggataaagg cgccaaaatt cttacaggcg gcaaacgtga tgggatgttc
      541 tatgacccga ttgtgcttgt caatgtagac cacagcatga agatcatgcg cgatgaaaca
      601 tttggtccgg ttatgcccat agttaaagtg aagaacgaag aagaagccat acacctcgca
      661 aatgacaatg cttatggttt aggcgcttcc atttggagca acgatatcag ccatgccaaa
      721 cgagttgcgg atcgggttga ggcgggatca atcatcatca atgatagtat cgctcaattt
      781 ggtgttccca tgctgccttt tggcggcaca aaggactcag gttttggtcg cacacacggc
      841 aaagaaggct tattacaatt tactcgccca tatagctttg ctattggcgg tgcgcccgtc
      901 aaaatggatg ttgcaaccgt tttgcgcgaa atgggacact atgagttagc aaccatgatt
      961 atgggagttc tatttggcac caccctcaaa caaaaagtag agaccttttt acaattcttc
     1021 cgtaggggca aaaaacaagc cccgccagcc ggcgagacca gttccaatac agctgcggct
     1081 gattaaaaaa ctgccctgta ggaattctac agggcagttt ttattctcaa tcttgcagac
     1141 caattatgtt cttctattca attccatcaa aaatatgatc aagcacatct tcgctcacat
     1201 cgaagacaga accattttct ggcaaagctt cttccatcat ccatttgggt aaacggtcat
     1261 cttctttggt aaaccctgcg cgtttgttga actcgcgctc tagtttaata gtctccttgc
     1321 caagagcttg taacacgtta tccggcacat cttcccaacc ataacgcgcc ttcaataggc
     1381 gtttcactac cccatcagga gtcgccgcgt aaccaaagcc tgcaaaaata catgcaccga
     1441 tggtatcgta gcccgccatg ttcaactgcg cgttgagaga cgcatccttt tgctgagtag
     1501 ggtcaaggtg attaacctga gcacggatcg ttaacccaca agtatggtct gcgccttgcg
     1561 gagtggtcgc ataagtcaca cccgtccctt taatggaacg cggttcatat gcggacatag
     1621 actgaccttt gaccgcagga acacgttcaa ctccatattt ctttccaacc gctactgccc
     1681 cgtctccaag gacacgtcca agttctgtgc cttttcgaat ttcatcaatc aatttcaagc
     1741 cgtcttcttc actgccccag cgcataattc ccgcttcggc agcgacccct aatgctccac
     1801 ctacttcgat ggtatccaaa ccaaggtcat taacttgcca attcatgcgt ccaatggagt
     1861 tgagcgaatt gatatcaagg ttggtgccca tcaaaccaac cgtctcatat tccaacggcg
     1921 aaacaatgat cttgccatct tcaccgccaa acacgtttga gcacttgatc gtacaccccg
     1981 ccatacaagc atgagagggg tcggagggtt tgccacgatt caaaataaac tcgcgcatgg
     2041 tttcgccgct aatcttttct acatcttcaa aagtgccacg cgagaaatta tgagttggca
     2101 acgctgcaaa attctgggtc atctgcggca tggcttgcgt accatactcg cggtatgtca
     2161 cagactgcgg atgattcatg accgat
//
LOCUS       DAOREF010000568         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38783_length_2185_cov_3.349765, whole genome shotgun sequence.
ACCESSION   DAOREF010000568 DAOREF010000000
VERSION     DAOREF010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38783_length_2185_cov_3.349765"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKI33_11475"
     CDS             <1..956
                     /locus_tag="PKI33_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNM37678.1"
                     /translation="EWIDGVPAVLEGWMMGQAGGAALADILFGRVNPSGKLAETFPLK
                     LTDTPSHINWPGGAGKVHYGEGIFIGYRYYDTKEIPVLFPFGHGLSYTTFEYSNAKVS
                     SKNFKDVDGLTVTVDVKNTGKLAGKETVQVYVHDQKSDLMRPVKELKGFAKVDLQPGE
                     TKTVSIHLDFRAFAYYHPEHKQWITEDGDFDILIGSSSADIRSHLTTTLQSTLDLPCV
                     LDIESTMREWMADRRGRQVLGSLYEQIESQVRRLFAGEDRYGNTKAEEQVAEQTVMGM
                     DIMDMMVDMPVVSVLMFQQSSLTMPAEEMVAGLLRQVHSMK"
     gene            1128..>2185
                     /locus_tag="PKI33_11480"
     CDS             1128..>2185
                     /locus_tag="PKI33_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37679.1"
                     /translation="MLNNHPLIQSLRELKGNPRITVWTEIMFGIPYNLFMPFFSVYML
                     ALGVTDQQIGSIASMGLVAQVFTALISGAIVDKFGRRLTLFIGDLLCWSVPCFIWAFA
                     QDVRFFVIAALMNSLWRISHTAWTCLMVEDAETRHLVHIWTWIMIFAFSSAFFAPLGG
                     WFVERYGLISAMRGLLIFGFVMLTAKGILLYIYSHETGRGIQRREETRHRSIISLLAE
                     YRSVFGQILHSRAILSALTLLVIMNIYSTITNNFWGVLFSSKLGFSDSQISTYVALRS
                     IVMTLGFFLIGPRLTNLRRFRLPLGAGFGVFLLSQVLLVLMPPKAVLFLVLSVVLEGA
                     GAALVNPMTESLLAVSMET"
BASE COUNT          503 a          493 c          528 g          661 t
ORIGIN      
        1 gtgaatggat tgatggtgtt ccggctgtgc tcgaaggttg gatgatgggg caggcaggtg
       61 gcgcggctct tgcagacatt ctctttgggc gtgtgaaccc cagcggcaaa ctcgcagaaa
      121 ccttcccgct caaacttacc gacactccat cccacatcaa ttggcccggc ggcgctggca
      181 aggttcatta tggcgaaggc attttcattg gttatcgtta ttacgacacc aaagaaatac
      241 ctgtactctt cccatttggg cacggtttga gctacaccac atttgaatat agcaacgcga
      301 aagtatcttc caaaaatttc aaagatgtgg atgggcttac ggtgactgtc gatgttaaga
      361 acacgggcaa actcgcaggt aaagaaacgg ttcaggtgta cgtccacgac caaaagtctg
      421 atctgatgcg ccccgtcaaa gaattgaagg gctttgccaa agtggacctc cagccgggcg
      481 agaccaagac cgtttccatc catttggatt tccgcgcctt cgcttactat caccccgaac
      541 acaaacagtg gattaccgaa gacggcgact ttgacatcct tattggctct tcttcagctg
      601 atattcgtag ccacctcacc accacgcttc aatcgacctt agatctgccc tgcgttctgg
      661 atattgaatc tacaatgcga gagtggatgg cagatcgacg tggtaggcag gtgcttggta
      721 gcctctacga acaaatcgaa agccaagtcc gcaggttgtt tgccggtgaa gaccgttatg
      781 ggaatacaaa ggcagaagaa caagttgctg aacaaactgt gatgggcatg gatatcatgg
      841 acatgatggt tgatatgcct gtagtgagtg tcttgatgtt ccagcaaagc tcactgacta
      901 tgcccgccga agagatggtc gctgggcttt tacgacaggt tcatagtatg aaatagctct
      961 tttgggatga aggttatcaa agccttcatc cctatttttt ttctaaaggc ttgatgtaac
     1021 ttgacgtggc gctgttcatt gcgaatagcg ccattattga cgctattttc aagcctgttc
     1081 aactggtttg acctatagaa agcttttggt aatattgcag gactacaatg ttaaataatc
     1141 atcctcttat tcaatccctt cgtgaactga agggaaaccc acgtattacg gtctggaccg
     1201 agatcatgtt cggtattcca tacaaccttt ttatgccatt cttttctgta tacatgctgg
     1261 ctttgggtgt caccgatcaa caaatcggta gcattgccag tatgggtttg gtagctcagg
     1321 ttttcaccgc gctcataagt ggcgccattg tcgataaatt tgggcgacgc ctgacgcttt
     1381 ttattggcga tctattatgc tggagcgtgc catgctttat ctgggcattt gcacaagatg
     1441 tgagattctt tgttattgcg gctttgatga atagcctttg gcgcatctca cacaccgcat
     1501 ggacatgctt aatggtcgaa gatgctgaaa cccgtcacct cgttcatatt tggacgtgga
     1561 ttatgatctt tgctttttct tcagcgtttt ttgcaccttt aggcggctgg tttgtcgaac
     1621 gatatggctt gatctctgcc atgcgcggat tgttgatctt cggttttgtt atgttgactg
     1681 ccaaaggtat tttgctttac atatattcgc atgaaacggg gcgtggcata caacgcagag
     1741 aagaaacccg tcaccgttct atcattagtt tgttggctga atatcgcagt gttttcggac
     1801 aaatattaca ttcacgcgcc atcctttctg cgctaacatt gctcgtcatt atgaatatct
     1861 actcaaccat cacaaataat ttttggggtg tgttgttttc cagcaaatta ggcttttctg
     1921 actcgcaaat ttccacctat gttgctttac gctccattgt aatgactttg ggcttctttt
     1981 tgatcggtcc acgcctcacc aatttgcgcc gatttcgttt gccgctgggc gcaggctttg
     2041 gtgtcttttt attgagtcag gtgttattgg ttcttatgcc gcctaaagct gtgttattcc
     2101 tggtgttgag cgtggttttg gaaggcgctg gggcagcgct tgttaaccct atgacggaat
     2161 ctttgctggc ggtttccatg gaaac
//
LOCUS       DAOREF010000569         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_38983_length_2179_cov_2.646422, whole genome shotgun sequence.
ACCESSION   DAOREF010000569 DAOREF010000000
VERSION     DAOREF010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38983_length_2179_cov_2.646422"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKI33_11485"
     CDS             complement(<1..415)
                     /locus_tag="PKI33_11485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNM37680.1"
                     /translation="MDSKRNWLDRPIHPTLPAITNEIILFVVIILLAIVTRFYNLGAR
                     VMSHDESLHTYFSWLLYQGRGYEHTPMMHGPLQFHLIALSYFLFGASDYTARIPAVLF
                     NLMTIWMVWYWRRYLGRTGALIAGFLLVISPFMLYY"
     gene            632..703
                     /locus_tag="PKI33_11490"
     tRNA            632..703
                     /locus_tag="PKI33_11490"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Met,seq:cat)
     gene            1051..>2179
                     /locus_tag="PKI33_11495"
     CDS             1051..>2179
                     /locus_tag="PKI33_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37681.1"
                     /translation="MFTWTYRKEVVTRIILITMVLVNAVVPTVARANSASEVENNGSA
                     KLIPPSSVPPVQPKIYYEPEETTLSNLQFSSDETEDTNIPQKDIVEFSIFASQGELSA
                     NGLITINVRLRNNGDTTVKDLSYFDKLKDIGSFQSSTDKHIEFNTITNTVIFTLESLA
                     VDEEILFTYDVKANNFGTNSISIHTADIEYTYEGEEKSASASMAIAENSPFVNSDSRI
                     LLPDQAGDGWSSSGQVSLYMGDGALTQDAVVVVEPEKLPGEGPELQFNLDVLQTASQT
                     KTSDGEIKEQDAKLLKETKVAFEEPVYLEINFDDVIDLSDIPADQEPYVATFDEGSGV
                     WVKVPIVTENAETNSVVVEAAHFSTWGAGLGNSLPKNGANVL"
BASE COUNT          660 a          361 c          522 g          636 t
ORIGIN      
        1 cgtagtaaag catgaaaggc gagatgacta aaaggaagcc tgcaatgaga gcgcccgtgc
       61 gacctaaata ccggcgccaa taccagacca tccaaatggt catgagattg aatagaacgg
      121 cagggatacg agcggtgtag tcgctggcac cgaagaggaa ataagaaagc gcgatcaggt
      181 gaaactgcag cggaccatgc atcatggggg tgtgttcata gccgcgtcct tggtacaaaa
      241 gccaggagaa ataggtgtgt aaactttcgt cgtggctcat gacacgtgcg ccaaggttgt
      301 aaaagcgcgt gacgatggca agtaagatga tcacaacaaa taggatgatc tcatttgtaa
      361 tcgcagggag tgtgggatga atgggacggt cgagccagtt tcgtttcgaa tccattgatg
      421 gcaccttgag tagagtgaaa tattttcgat aaggcttaaa taatagtctt ttaagtttaa
      481 tatgacgtga gaaggtatta taacaggagg ggttgaaaat cttctttgcc ttggttttgg
      541 gcttaacctg caaggttcat ggaaataggt tttgtttctt gcttgacttt tctatggtta
      601 cttggtaata tcggcgggct ttactaaccg atgcggggtg gagcagtggc agctcgtcgg
      661 gctcataacc cgaaggtcac aggttcaagt cctgtccccg ctactctaaa gaccgccaga
      721 ctgtttacag tctggcggtc tttaattttt aagtaaaaaa ttttgaattt aaaaataaat
      781 ttggatgaca tactccaata attttttaag gttgattaac ttgacattaa tgttagcaaa
      841 taggtaatat catattgtat ttataagatt gcccagggga ctggggagta tgagaattat
      901 ggctcaaccc aagtagattt acaaaataat aatgtcggct gtggtgagca gccccgcaaa
      961 gattaatttt ttctaggttt attacagcct agttaattag ttactatttg gttttggtgg
     1021 gtatatgcca aaatctcttt cggaggatca atgtttacat ggacatatcg aaaagaggtc
     1081 gtaacaagaa ttatcttaat aactatggtt cttgttaatg ctgtagtacc aactgttgct
     1141 agggctaact cggctagcga agttgaaaat aatggatcag caaaactgat cccaccctca
     1201 tctgtccctc ctgttcaacc taagatttac tacgagccag aagagacgac gctttcaaat
     1261 cttcaatttt catcagatga gactgaagat actaatattc cgcagaaaga tatagtagag
     1321 ttttcgatat ttgcttctca gggggagcta agtgcaaatg gtttaatcac tattaatgtg
     1381 agactgcgaa acaatgggga tacaaccgtt aaagatttaa gctattttga taaattaaag
     1441 gatataggta gctttcagtc ttctacagat aaacatattg agtttaatac tattacaaac
     1501 acagtaatat ttactctaga gtctcttgct gttgatgaag agattctgtt tacgtatgat
     1561 gtgaaggcca ataactttgg gacaaattcg atttctattc atacggcaga tattgaatac
     1621 acctatgagg gagaagaaaa gagcgcttct gcgtccatgg cgattgcgga aaatagtcca
     1681 tttgtgaatt cagattctcg aatccttcta cctgatcagg ctggagatgg ttggagttcg
     1741 agtggacaag tttcattata tatgggagat ggagctttaa ctcaagatgc agttgttgtt
     1801 gttgagcctg aaaagctacc aggagaagga cctgagcttc aatttaatct agatgtattg
     1861 caaacagcat cacaaaccaa gacttcagat ggtgagatta aggagcaaga cgcgaaatta
     1921 ttaaaagaga ctaaagttgc ttttgaagag cctgtttatc ttgagattaa ttttgatgat
     1981 gtaatagatc ttagtgatat tccagcagat caagagcctt atgttgctac gtttgatgaa
     2041 gggagtggcg tttgggtaaa agtacctatt gttactgaga atgcggaaac aaattctgta
     2101 gttgttgagg cagcacattt ttctacttgg ggagctgggt tgggaaattc tcttccaaag
     2161 aacggagcaa atgtattgc
//
LOCUS       DAOREF010000570         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39036_length_2177_cov_3.933553, whole genome shotgun sequence.
ACCESSION   DAOREF010000570 DAOREF010000000
VERSION     DAOREF010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39036_length_2177_cov_3.933553"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKI33_11500"
     CDS             complement(<1..401)
                     /locus_tag="PKI33_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37682.1"
                     /translation="MNESSPLIKEQSMAAVITRRFFKHKLAGVAITVLALLVLFSALA
                     SLTPYEPTEQNPTNIFQKASGVHWFGTDELGRDVFTRILYGGRVSLAVGLLSTLLSIS
                     LGVVMGALSGYFGGLLDSTLMRITDAFLTFPT"
     gene            complement(394..1368)
                     /locus_tag="PKI33_11505"
     CDS             complement(394..1368)
                     /locus_tag="PKI33_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37683.1"
                     /translation="MGTYALRRILQTIPILFIISILLFLMVRAAPGGPLTAARRNPNI
                     SKEQIEAIEEKLGLNDPLPVQYGRWLGDMLRGDMGDSIKFRRPVSEMIGERVPNTLLL
                     VGASFLVTLLFAIPIGILSARKPYSKFDYAMTTVTFIGQAIPVYWLGLGLIVIFYVTI
                     KNPFTGNPLFPVGGMNTRGMEGNLLDTLWHLVLPVTALSLGWVAWYSRFLRSSMLDVL
                     HEDYVRTAHAKGLTDSLVYYKHALQNAILPLVTLIALDLPSLFAGALFVETIFSWPGM
                     GRLFWDAAKGRDYPVLLGVVMLTAVLIILCNIIADLAYGWLNPQVKYE"
     gene            complement(1377..>2177)
                     /locus_tag="PKI33_11510"
     CDS             complement(1377..>2177)
                     /locus_tag="PKI33_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNM37684.1"
                     /translation="EAAEMAKVKGEFVRGGAAKFFMDGVWESYTALNVDPYADVPDAK
                     PDGIYSLEHFTRMATLCDKMGMQIFVHCCGDGAVRRTLDGYEAVQKANGKRDSRHRIE
                     HIEVYHPDDLPRFKQLGVLPSMQTSHAPFSIAEGDVWPTRVGKQRWHLSFAWRDILNA
                     GNQIAFGSDWPVAPYSPMINLHVGLNREPWAEGVPSHNLTLEELIIGYTRDAAYAEFM
                     ENEKGQIKEGYLADLVLFSHDLFALPKENIMETKPVLTMVNGKVVYEA"
BASE COUNT          589 a          555 c          540 g          493 t
ORIGIN      
        1 gtggggaagg ttaggaaggc atctgtgata cgcatcaggg tcgagtcgag caggccgcca
       61 aagtagccac ttaatgcacc catgacaacc cctaacgaaa tggataacaa agtggatagc
      121 aagccaactg ccaacgagac tcgcccgcca tataagatgc gagtgaacac atcccgacca
      181 agttcgtcgg tgccaaacca atgaacgccg gatgcttttt gaaaaatatt cgtcggattc
      241 tgttctgtgg gttcataggg tgttaggctt gccaacgctg agaacaacac caataatgcc
      301 aagactgtaa tggcaacacc cgccagtttg tgcttgaaga agcgacgcgt gatgacggct
      361 gccatgcttt gttctttgat aagcggactc gactcattca tatttcacct gtgggttgag
      421 ccagccatac gccagatctg caataatgtt gcagaggatg ataagaacag ctgtgagcat
      481 cacgacccca aggaggacgg ggtaatctcg cccctttgcc gcgtcccaaa agagacgccc
      541 cataccgggc caggagaaga tggtttctac aaataatgca cccgcaaaaa gactgggcaa
      601 gtcaagcgcg atcaaggtga ccagtggcaa aatcgcattt tgcaaagcat gtttgtaata
      661 gacaagacta tctgttaacc cttttgcatg tgccgtgcgg acgtaatctt cgtgtaacac
      721 gtctaacatg ctggagcgta gaaaccgtga ataccaagcg acccatccca aactgagggc
      781 ggtaacgggc aagaccaaat gccagagcgt gtctagcaga ttcccttcca tgccacgggt
      841 gttcattcca ccaaccggga aaagcggatt ccccgtgaac ggatttttga tggtgacata
      901 aaagatcacg atgagtccca gccccagcca ataaactgga atcgcctgcc caataaaagt
      961 aaccgtcgtc atggcgtaat cgaatttgga gtagggtttt cgagcggaga gaatgccaat
     1021 ggggatggca aataacaagg tcacaagaaa agaggcgccc accaataaca gagtattggg
     1081 cacgcgttca ccgatcatct ctgaaaccgg gcggcgaaac ttgatcgagt cgcccatatc
     1141 accgcgcagc atatcgccga gccaacgtcc atattgaaca ggtaaaggat catttaatcc
     1201 gagcttttct tcaatggctt caatctgttc cttcgaaata tttgggttcc tgcgagcggc
     1261 tgttaacggt ccgcccggcg cggcacgcac catcagaaac aataaaatac tgatgatgaa
     1321 aagaatggga atggtttgta aaatgcgacg cagggcgtaa gttcccatga gtacctctag
     1381 gcttcgtata cgaccttgcc atttaccatg gtcaagaccg gcttggtttc cataatgttt
     1441 tctttgggca aagcaaatag gtcatgcgag aacaagacca aatctgcaag atacccttct
     1501 ttgatttgac ctttttcatt ttccataaac tcggcatatg ccgcatcgcg ggtataccca
     1561 atgataagtt cttcaagggt caggttgtgg gatggcacac cttctgccca aggttcacga
     1621 ttcaaaccga catgtaaatt gatcatcggg ctgtagggcg ctacaggcca gtcactgccg
     1681 aaggcaatct gattgccagc attcaaaata tcgcgccagg caaacgaaag atgccaacgt
     1741 tgcttgccta cccgtgtggg ccatacatct ccctcggcaa tgctaaaggg agcatggctg
     1801 gtttgcatgg atggcaacac gcccaactgt ttgaagcgtg gcaggtcatc gggatgatag
     1861 acctcaatgt gctcaatgcg gtgacgacta tcacgcttcc cgttcgcttt ttgaacggct
     1921 tcgtacccat cgagcgttcg cctcactgca ccatcgccgc aacaatggac aaagatctgc
     1981 atgcccattt tgtcgcatag ggttgccatg cgtgtgaaat gttcgagcga ataaatgcca
     2041 tcaggtttgg catctggcac atcggcgtaa gggtcaacgt tgagcgctgt atatgattcc
     2101 catacaccgt ccataaagaa tttcgccgcg ccgccacgca cgaactcgcc tttgaccttt
     2161 gccatctcag cggcttc
//
LOCUS       DAOREF010000571         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39136_length_2174_cov_3.680510, whole genome shotgun sequence.
ACCESSION   DAOREF010000571 DAOREF010000000
VERSION     DAOREF010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39136_length_2174_cov_3.680510"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1329)
                     /locus_tag="PKI33_11515"
     CDS             complement(124..1329)
                     /locus_tag="PKI33_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNM37685.1"
                     /translation="MEKDSRLPVTVLSGFLGSGKTTVLNHILNNREGLHVAVIVNDMS
                     EVNIDAQLIRDGGAELSRTEEKLVEMTNGCICCTLREDLLLEVTRLAGEKRFDYLLIE
                     STGIGEPLAVAATFDFPMNEDDTNLSDIARLDTMVTVVDASNWLRDYLEAKSLKELGM
                     AVSDEDGRTMVDLLIDQVEFADVILVNKVDLVTEEELGKLIAILKKLNPDTAVVKTEF
                     GKAPLNFILNTGLFNMEKSAALPQWADELAGRHNPETEEYGISSLTYQANLPFHPERL
                     MEFLQNGGLDSIVRSKGYFWLASRMDHALGWSQAGTQTTINLAGLWWAAVPKKQWPRA
                     AELRKFIQSNWQEPYGDRRQELVFIGQKLQKDELKQALDQCLMTEMEMKNIKKLKITD
                     PFPSLQPNN"
     gene            complement(1342..1584)
                     /locus_tag="PKI33_11520"
     CDS             complement(1342..1584)
                     /locus_tag="PKI33_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HNM37686.1"
                     /translation="MARKLNHKTRKALAPKTRFGKNISFSQRHTPRKFKPNVQTITLW
                     INGKPIRVKLSARQIRSLGKQEQPKKLMTELRKLAK"
     gene            complement(1584..1748)
                     /gene="rpmG"
                     /locus_tag="PKI33_11525"
     CDS             complement(1584..1748)
                     /gene="rpmG"
                     /locus_tag="PKI33_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNM37687.1"
                     /translation="MAKKKDIRIVVKMQSTESQYMYYTQKSRRNNPQRMELRKFDPIV
                     KKHVLFKESK"
     gene            complement(1843..>2174)
                     /locus_tag="PKI33_11530"
     CDS             complement(1843..>2174)
                     /locus_tag="PKI33_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNM37688.1"
                     /translation="LVTNHEALGYFAERYGFEVVDTILPSFSSEASASAQEIAAAVDA
                     VKNSGAPAIFLGEVENADLANQIAAETGVKVVDTLYLESLTDGEPAATYIDMMKHNVT
                     QIIEGLK"
BASE COUNT          538 a          499 c          476 g          661 t
ORIGIN      
        1 ccgtagccga tgctgatgtt ttttacttca agacgatggg gtgtatgaga catttgtatc
       61 gaatgacgct ttcaatttta ttctatctta cttctagttg aacatagatg tatcagatta
      121 gtgttagtta tttggctgaa gagacggaaa tgggtctgtg atctttaatt tcttgatatt
      181 tttcatctcc atttcggtca ttaagcattg atctaatgcc tgctttaatt catccttttg
      241 cagtttttga ccaataaaga cgagttcttg tctgcgatca ccatacggct cctgccaatt
      301 ggattgaatg aattttctca attcagccgc tctgggccat tgctttttag gaacagctgc
      361 ccaccatagc cctgccagat tgatggttgt ttgagtgcct gcctgtgacc aacccaaggc
      421 atgatccata cgagatgcaa gccaaaagta gccctttgag cgcacaatac tatccaaccc
      481 gccgttctgt aaaaattcca taaggcgttc tgggtgaaaa ggcaggttgg cttgataggt
      541 aaggctacta attccgtatt cttccgtttc ggggttgtgg cgccctgcca gttcatctgc
      601 ccattgtggc agcgcagcag atttttccat gttgaatagc ccggtattaa gaatgaaatt
      661 caatggtgct ttaccaaatt ctgttttgac cacggctgta tctgggttca acttcttgag
      721 aatggcgatc aatttcccta gttcttcctc ggtaacgagg tcaactttat tgaccaagat
      781 cacatcagca aactcaacct gatcgatgag taaatccacc atggtacgac catcttcatc
      841 gctgactgcc atgcccaatt ccttcaagct tttggcttca aggtaatctc gcagccagtt
      901 ggatgcatcc acaacggtta ccatggtatc gaggcgggca atgtcggaga gattggtatc
      961 atcttcattc attgggaaat cgaatgtcgc agccactgcc agcggctcac cgatcccagt
     1021 cgactcaatg agcagataat caaaccgctt ttcgccagca agccgggtaa cttccaggag
     1081 caggtcttcg cgcagggtac aacagataca gccattggtc atttcgacca gtttctcttc
     1141 tgtgcggcta agttctgcgc caccatcgcg gatcagttgc gcatcgatgt tgacttcgct
     1201 catatcattg acaataaccg ccacatgtag accttcacgg ttgttgagaa tatggtttag
     1261 cacagtggtt tttcctgagc ctaaaaagcc ggaaagcaca gtaacaggta aacgagaatc
     1321 tttttccatg gtgcaatcct tttattttgc aagcttacga agttctgtca ttaatttttt
     1381 aggttgttct tgtttgccaa gcgaacggat ttgcctggca gataacttca cacggattgg
     1441 ctttccattg atccataacg taatggtttg tacgttcggc ttgaatttac gtggagtatg
     1501 gcgttgagaa aaacttatat tctttccgaa acgtgttttg ggagctaacg ccttgcgggt
     1561 tttgtgattc aatttgcgtg ccattatttg ctctccttga ataaaacgtg cttttttacg
     1621 atcgggtcaa acttacgaag ttccatcctt tgagggttgt tgcgacggct cttctgggtg
     1681 taatacatgt attggctttc tgtgctttgc atctttacaa cgatgcggat gtcttttttc
     1741 tttgccatgt gtgcctctaa atgaaaatga ttttcaataa gaaattcgat aaaaagacag
     1801 ggagcctatg aatctccctg tctcagagaa aattacagag tcttacttca aaccttcgat
     1861 gatctgcgtg acattatgct tcatcatgtc aatgtaggtc gcagcgggct caccatcagt
     1921 gagtgattca agataaagcg tatctaccac tttgacaccc gtttcagcgg cgatctgatt
     1981 ggcaaggtct gcattttcta cttcgccgag gaagatggca ggcgcaccag aattcttgac
     2041 cgcatccacc gccgcagcga tctcttgagc agaagcgctt gcttcagaac taaagctcgg
     2101 caaaatagta tccacaacct caaagccata gcgttctgca aaataaccca acgcctcatg
     2161 gtttgtgacc aaca
//
LOCUS       DAOREF010000572         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39171_length_2173_cov_4.788008, whole genome shotgun sequence.
ACCESSION   DAOREF010000572 DAOREF010000000
VERSION     DAOREF010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39171_length_2173_cov_4.788008"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /gene="ppk1"
                     /locus_tag="PKI33_11535"
     CDS             <1..1937
                     /gene="ppk1"
                     /locus_tag="PKI33_11535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNM37689.1"
                     /translation="DEFFMVRVSGIRKQVEANIMKLSEDGLTPREELAGIRKTAQDLM
                     QDAQQTFHRKLLPKLEKEGIHVLDYSKLSKSQKERADQYFKDVVYPVLTPLALDPGHP
                     FPHISNLSLNLAIVIRDKKGNEKFARLKVPDTLPRLIPIKKSSGSARKDGTIPFHHYF
                     VWLDQVIAANLDGLFPGLEVVSAHPFRIVRDADIEIQELEADDLLETMQQNIRKRKFG
                     SVVQVAVHPSMPEEMRDLLVDNLEITRNDIYSLPHPLGLASLWQLYNSVERHDLKYPP
                     YIQRTPKPLRNLDTSSEIFDAIRNENVLLHHPYESFIPVVDFLYAAARDPHVLAIKQT
                     VYRVGSNAPVVEALLEAAERGKQVAVLVELKARFDEESNIGWARALEQAGVHVVYGLV
                     GLKTHCKVAMVVRKEGDGIRRYLHLATGNYNAVTSRIYEDLGMFTCDEDMGADATDLF
                     NYLTGYSTKKSYKKLLVAPVNLREQMEKLIRREIEHAKAGNKARIIFKMNSLVDMDMI
                     QLLYQASQAGVKVDLLIRGMCCLRPGIKGVSENIRVLSVVGRYLEHSRIYYFQNNGND
                     EIYMGSADLMPRNLNHRVEVVFPVENKGQVKFLRDKILEAYLKDNTRARILKTNGTYE
                     RSKPDSNGKRFDVQSHLMGE"
BASE COUNT          591 a          501 c          512 g          569 t
ORIGIN      
        1 tggacgagtt ctttatggtg cgtgtctctg gcatccgcaa acaagtggaa gccaacatca
       61 tgaagctttc tgaagatggg ctaactccgc gtgaggaact ggcaggcatt cgcaaaacag
      121 ctcaagacct gatgcaagat gcccaacaga ccttccatcg caagttgttg cctaagcttg
      181 aaaaagaagg cattcacgtt ctggattatt ccaagctcag caaatctcag aaggaacgcg
      241 ccgatcagta cttcaaggat gttgtctatc cggttctgac tccgctcgcg cttgatcctg
      301 gacacccatt cccgcatatt tccaacctga gccttaatct cgccatcgtc atccgcgata
      361 aaaagggcaa tgaaaaattt gcacgcctca aagtgcctga cactttaccg cgcttgatcc
      421 ccatcaaaaa atcttctggc agcgcccgca aagatgggac gatccccttc caccattatt
      481 tcgtttggtt agaccaagtc attgctgcca atctcgatgg gctttttcct ggcttggaag
      541 tggtctctgc acatcccttc cgcattgtcc gcgatgcaga tattgaaatc caagaactcg
      601 aagccgatga cctgttagag accatgcaac agaatattcg taaacgcaaa ttcggctcgg
      661 tagtacaggt agcagttcac ccatccatgc cggaggaaat gcgtgacttg ctggtggata
      721 accttgaaat tacccgaaat gatatttatt cattgccgca tccgcttggg ttggcgagtc
      781 tttggcagtt gtataacagc gtcgaacggc atgacttgaa atatccgcct tatattcaac
      841 gcacgcccaa gccactccga aatctggata catcttcaga gatatttgat gcgattcgga
      901 atgaaaatgt tttgcttcat cacccttacg aatcgttcat cccagtcgtg gattttcttt
      961 atgccgccgc gcgcgatccg catgtgcttg ccatcaaaca gacggtgtat cgggtagggt
     1021 ccaatgcgcc tgtggttgaa gctctgctag aagcagcaga acgcggtaaa caagttgccg
     1081 tactcgtgga gttgaaagcc cgctttgatg aagaatcaaa tattggttgg gcgcgagcct
     1141 tggagcaggc aggagttcat gttgtctatg gcttggtagg gttgaagacc cactgtaaag
     1201 tagccatggt tgtccgcaaa gaaggggatg gcatccgcag atatttgcat cttgccaccg
     1261 gcaattacaa cgcggtcaca tcccgcattt atgaagattt gggcatgttc acttgcgatg
     1321 aagatatggg cgccgatgcc acagacttgt ttaactacct cactggttac tccaccaaga
     1381 aaagttataa gaaattgttg gttgccccgg tcaatttgcg tgagcagatg gagaaactga
     1441 ttcgacgtga gatcgaacac gcgaaagccg gaaacaaggc acgcattatt ttcaagatga
     1501 attcactggt tgatatggat atgatccagc ttctctatca ggcttctcag gctggcgtca
     1561 aagtggattt gctcattcgc ggcatgtgct gtttgcgccc cggtattaag ggcgtcagtg
     1621 aaaatatccg cgtgctaagt gttgtcgggc gttaccttga acatagccgt atttattact
     1681 tccaaaacaa tggcaatgat gaaatttata tgggtagtgc tgatcttatg cctcgcaacc
     1741 ttaaccaccg tgtcgaagta gttttcccag ttgaaaataa gggacaagtc aaattcttgc
     1801 gcgataagat tctggaagcc tatctcaaag ataatacccg ggcgcgcatt ctaaaaacga
     1861 acggtaccta tgagcgttcc aaaccagatt ctaatgggaa gcgtttcgat gtccaatctc
     1921 atttgatggg tgagtagaaa ccataacctt ttgttaacaa tcaataatgt taacgttcat
     1981 ataatgaacc atcttcaaaa caaaggtgac ttctatggaa ttaaacgtca gcgatttaga
     2041 taagggtatt acactaatca aattagacgg caggctagat attaccggtg taaatcagat
     2101 cgaaacaaaa tttgcggcat attgctccgg tgaaaatgca cgtgtgatcg tcgatatggc
     2161 aaaggtggac tat
//
LOCUS       DAOREF010000573         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39252_length_2171_cov_2.847826, whole genome shotgun sequence.
ACCESSION   DAOREF010000573 DAOREF010000000
VERSION     DAOREF010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39252_length_2171_cov_2.847826"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKI33_11540"
     CDS             <1..429
                     /locus_tag="PKI33_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM37690.1"
                     /translation="AIAERGVINEPKELTVTREYRDKLNIKCSSILQLALNLSGGNQQ
                     KVVLSKWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLANEGKAIILISSELPEVLG
                     VCDRIYVMREGKIVGEMPAKEASQEIVMKHIMFDQEAIAQ"
     gene            435..1589
                     /gene="gguB"
                     /locus_tag="PKI33_11545"
     CDS             435..1589
                     /gene="gguB"
                     /locus_tag="PKI33_11545"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM37691.1"
                     /translation="MSNLFKNNIREYGMLIALIVVMIFFYFVTDGILMKPINITNLVL
                     QNSYVIIMALGMLLIIVSGWIDLSVGSVAAFIGAIAGVMIVKWGWNWGLVIAICIFVG
                     GLIGAWQGYWVAYLKIPAFIVTLAGMLVFRGLTLVMVRGEAIGPFPEGFRNISGGYIP
                     DFFHYEGFHLTTIIVGIIVSIFLILSDMRTRKNQEKYGFEVTPLYFFIIKQVLLTAAV
                     MGFCYLMASYKGIPNLLVIMFPLVAIYSFITTQTVLGRRIYALGGNEKAARLSGVNTQ
                     RLVFLTFVNMGALAALAGTVVASRLNSSTPNAGEGFELDVIAACFIGGASASGGIGKV
                     IGAVVGAFLVGVLNNGMSILGVGIDWQKVVKGLVLLLAVLFDVYNKSKSS"
     gene            1748..>2171
                     /locus_tag="PKI33_11550"
     CDS             1748..>2171
                     /locus_tag="PKI33_11550"
                     /inference="COORDINATES: protein motif:HMM:NF002696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HNM37692.1"
                     /translation="MEDILKNLLEQEQELQFTKFNEVTAWQLGSQMVERAMRDNLPIT
                     IDITRGQQQLFHYSMPGTAADNDEWVKRKVRLVNRFGHSSFYMGQLLKHKGKTIEQSY
                     LISESEFAAHGGCFPIIVKGTGMVGTITVSGLPQEEDHK"
BASE COUNT          559 a          468 c          519 g          625 t
ORIGIN      
        1 gctattgcgg aaagaggcgt gatcaatgag ccgaaagagc tcacagtcac ccgcgaatac
       61 cgtgacaagc taaatatcaa atgttccagt attttgcagt tagcattgaa cctttcaggt
      121 ggaaaccaac aaaaagtagt tttgagtaaa tggttattct ccaacccaga tgttcttatt
      181 ctggatgaac ctactcgtgg cattgacgta ggtgccaaat acgaaatcta tacgatcatt
      241 aatcagctag ccaacgaggg caaggcaatc attttgatct cttcagaatt gcctgaagtg
      301 ttaggggttt gtgaccgtat ttatgtgatg cgagaaggaa agatcgttgg tgaaatgcct
      361 gctaaagagg catcgcaaga aattgtaatg aagcacatta tgtttgacca agaggcgata
      421 gcacaatgaa aactttgtct aaccttttta agaataacat ccgtgaatac ggtatgttga
      481 tcgcgttgat cgtggttatg atctttttct acttcgtaac agatggtatc ttaatgaaac
      541 cgatcaacat taccaatctg gtactccaga acagctatgt gatcattatg gcattgggta
      601 tgttgttgat catcgtttcc ggctggatcg acctttctgt cggttcggta gcagctttca
      661 ttggcgcgat cgctggtgtg atgatcgtca aatggggctg gaactggggc ttggtcatcg
      721 ccatttgtat ctttgttgga ggcttgatcg gagcatggca agggtattgg gttgcttatc
      781 tcaagatccc cgcctttatt gtgactctgg ctggcatgtt ggtcttccgc ggtttgaccc
      841 tggttatggt ccgcggtgaa gcgattggtc ctttccctga aggctttcgc aatatcagtg
      901 gtggttacat cccagatttc ttccactatg aaggcttcca tcttacaacc atcatcgttg
      961 gaattatcgt ttcgatcttc ttgatccttt ctgatatgcg aacccgaaaa aatcaggaaa
     1021 aatacggttt cgaagtcaca ccattgtatt tcttcatcat caagcaggtc ttgcttacag
     1081 ctgctgtgat gggcttttgt tatctgatgg cttcctacaa gggcattcct aatttgctcg
     1141 taatcatgtt cccgttggtt gccatttact cctttatcac cacccaaacc gtgctcggac
     1201 gtcgcatcta cgcacttggt ggaaatgaaa aagcagcccg cctctctggc gtcaacacgc
     1261 aacgccttgt attcctcact tttgtgaata tgggtgcgct tgctgccttg gctggcacag
     1321 tggttgcctc gcgcttaaac agctccactc cgaatgcggg tgaaggtttt gaattggatg
     1381 tgattgcagc atgtttcatc ggtggtgctt ctgcaagcgg tggtattggt aaggtcatcg
     1441 gtgctgtagt gggagccttc ctcgtaggtg tgctcaacaa cggcatgtcc atcctcggcg
     1501 taggtattga ctggcaaaaa gttgttaaag gtttagtgct cctgctcgcc gtactctttg
     1561 atgtgtataa caagagcaag agctcataat tcatttttca caaaggctca gtcgtaacga
     1621 ctgagccttg tataaatcgc ataaagactt tcgaagaaat ttaatattta ataaaaaagc
     1681 gaattcagtt gtcgttttgg ttttgtttct cattttcagg caaacgagcc acataaagga
     1741 taaatttatg gaagatatac tgaagaattt acttgaacaa gagcaggaac ttcagttcac
     1801 aaaattcaac gaagtcaccg catggcaatt gggaagccaa atggtggaac gcgccatgcg
     1861 agacaacctc cccatcacca tcgacatcac gcgtggacag cagcaactct tccattacag
     1921 catgcctggc accgccgcgg acaatgacga gtgggtcaag cgcaaggtaa ggctggtgaa
     1981 tcgtttcggt cacagttcgt tctatatggg gcaattgctt aaacacaagg gcaagacaat
     2041 tgaacagtct tacttaatat ccgaaagtga atttgccgcg catggcggct gcttccctat
     2101 tatcgtaaaa ggcacaggca tggtcgggac gatcaccgtc tctggcttgc cacaggaaga
     2161 agatcataaa t
//
LOCUS       DAOREF010000574         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39366_length_2167_cov_4.051610, whole genome shotgun sequence.
ACCESSION   DAOREF010000574 DAOREF010000000
VERSION     DAOREF010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39366_length_2167_cov_4.051610"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1311)
                     /locus_tag="PKI33_11555"
     CDS             complement(52..1311)
                     /locus_tag="PKI33_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HNM37693.1"
                     /translation="MSLNLDVIRQQFPSLNRNAIFFDNPGGTQVAKQSIDRITKYLIE
                     NNANHGGVFATSIASDEVLDEAHRAMADFYNAPSAEEIVFGNNMTTITLHISRSISRD
                     WKEGDEIVLTRLDHDANVTPWVLAAQDKGVKINWVDFDVEDGTLKLNDLQKALERKPK
                     QVAVGYASNSLGTVNPVQKIVKMAHDAGALVYVDAVQYAVHGAIDVQKLNADFLVSSS
                     YKYFGPHAGILYGKRELLEKLVAYKVRPATNELPGKFETGTQNHEGIAGILGAIEYFE
                     WVGKEFGGEYADELKQQGYSGRRLTLKQAMSAIHAYEFELSRVTLSTLEAIPGIHIFG
                     NTDPRRLDERIATFSFRIGDMNPQTIAEKLAAENIYVWNGNYYAINVSERLGVEEKGG
                     MVRVGPVHYNTVEEINKLGDVLKKIAG"
     gene            1387..1809
                     /locus_tag="PKI33_11560"
     CDS             1387..1809
                     /locus_tag="PKI33_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNM37694.1"
                     /translation="MFERILLAVDGSEHALHATRKAAEMARCMKPTEFRIVVAYDSIP
                     LYLGEPNMQLVITNRKGEAEEILNVAVKEVGDIPCEIHTEIIEGDPASAILEVAKIRN
                     SDVIIMGSRGLGRLAGLLLGSTSQKVVSHAPCPVLIVR"
     gene            complement(1839..>2167)
                     /locus_tag="PKI33_11565"
     CDS             complement(1839..>2167)
                     /locus_tag="PKI33_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37695.1"
                     /translation="VVACDMPFASKSFFESAHRLLVKEEADVLIAKTEAGYEPFHALY
                     RRETCLPAIEAAIEADQWKAVAWISQVRARVLSPEELKAYDPSGLCFWNVNTPEEFAK
                     AEQLAK"
BASE COUNT          522 a          535 c          534 g          576 t
ORIGIN      
        1 tgtaatatct ttaaataaaa actcccagtc attcgactgg gagttttttg tttagcctgc
       61 aattttcttt aacacatcgc ctagtttgtt gatttcttcc acagtattgt agtggacggg
      121 tcccacacgc accatgccgc ctttttcttc cacccccaac cgttcagaga cgttaatcgc
      181 gtagtagttg ccattccaca cataaatatt ttcggcggcg agtttctcag caatggtctg
      241 cggattcatg tcgccgatgc ggaaggagaa ggtcgcgata cgttcgtcca accggcgcgg
      301 atcggtattg ccgaaaatat gaattcccgg aatcgcttcg agtgtggaaa gcgtcactcg
      361 gctgagttca aattcataag catggatggc actcatggct tgcttgagag ttaggcgacg
      421 tccgctatag ccttgttgct tcaactcgtc agcgtattca ccaccgaatt ctttgccaac
      481 ccattcaaag tattcaatgg caccgagaat gcctgcaatg ccttcgtggt tttgggtgcc
      541 ggtctcaaat ttgccaggca attcattcgt ggctgggcgc actttgtagg caacgagttt
      601 ttcaagcaat tcgcgcttgc cgtacaaaat ccctgcatgt ggaccgaagt acttataaga
      661 agaagacacc agaaaatcag cgttgagctt ttgcacgtca atcgcgccgt ggacggcgta
      721 ttgcaccgca tctacataca ccaatgcacc cgcgtcgtgt gccattttaa cgatcttctg
      781 aaccgggttg accgttccca gcgagttaga ggcataccca acagcgacct gtttcggctt
      841 acgttcgagc gctttctgca ggtcattcaa tttgagcgtg ccatcttcca catcaaagtc
      901 tacccagttg attttcacgc ctttgtcttg cgcggctaac acccacggcg tgacgttggc
      961 atcatggtcg aggcgggtca gcacaatttc atcgccttct ttccaatcgc gcgaaatgga
     1021 gcggctgatg tgcagcgtga tggtggtcat gttgttgcca aagacgatct cttcagccga
     1081 aggggcgtta tagaaatctg ccatggcgcg atgtgcttca tcgagcactt catcagaagc
     1141 aatactggtg gcaaagactc cgccatgatt ggcgttgttc tcgatcagat acttggtgat
     1201 gcggtcgatg gattgtttgg caacctgagt cccgccgggg ttgtcgaaga aaatggcgtt
     1261 gcgatttaaa gatggaaact gctgacgaat gacatcgaga tttagtgaca tatagggatg
     1321 atcctccaat gaaattcaaa cacgttcgct gtatactgaa attgtacccc acggaaagga
     1381 gcttccatgt ttgaaagaat tcttctcgct gtggatggtt ccgagcacgc cctccacgcc
     1441 actcgcaaag ccgccgaaat ggcgcgctgc atgaaaccca ctgagtttcg tattgttgtt
     1501 gcatatgatt ctattccact ctatctgggg gagcctaata tgcaattggt catcaccaat
     1561 cgtaaaggcg aagccgaaga gatattgaat gtcgctgtaa aagaagtcgg tgacattcca
     1621 tgtgaaatcc acaccgaaat tattgaaggc gaccccgcca gcgccattct tgaagtggca
     1681 aagattcgta atagtgatgt catcatcatg ggctcacgcg ggttaggcag gctggcaggc
     1741 ttactgcttg gcagtaccag tcagaaagtt gtctcacatg ccccatgccc ggtgttgatt
     1801 gttcgataaa caaaaaagta ggtcacgcat tgcgtgacct actttgctaa ttgctcggct
     1861 tttgcaaatt cttcgggcgt gttcacgttc caaaaacata agcccgaagg gtcgtaagct
     1921 tttaactctt cgggggaaag aacgcgtgcg cggacttgtg aaatccacgc cacagctttc
     1981 cattgatcgg cttcaatggc ggcttcaatg gcaggtagac aggtctcacg gcgatataaa
     2041 gcatggaacg gttcatagcc tgcttctgtt tttgcgatga gaacatccgc ctcttcttta
     2101 acaaggagcc tgtgtgcgct ttcaaaaaag cttttactgg cgaaaggcat atcgcatgcc
     2161 accaccg
//
LOCUS       DAOREF010000575         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39368_length_2167_cov_3.845170, whole genome shotgun sequence.
ACCESSION   DAOREF010000575 DAOREF010000000
VERSION     DAOREF010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39368_length_2167_cov_3.845170"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PKI33_11570"
     CDS             complement(<1..633)
                     /locus_tag="PKI33_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNM37696.1"
                     /translation="MAEKSQAHDKQVLHKLGYAQELSRRMSGFSNFAISFSIICILAG
                     GISAFPAAFNALGSGGAFLIWLVGGVLAMSVAVGMGQIASSFPTAGGLYHWSSHLGGK
                     FWGWATAWFNLIGLICVVSSVDVLLYTVFFRDLLFGTALGVDVSTWGTTHQTIFLIIA
                     LGTQAILNHYYIDITTKLTDWSGYVILGLTVILIITLFAFSPVPLDFSRLW"
     gene            complement(879..1637)
                     /locus_tag="PKI33_11575"
     CDS             complement(879..1637)
                     /locus_tag="PKI33_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNM37697.1"
                     /translation="MSKIKAVFFDQDGVIIDTERDGHRVSFNMTFKEFGFTDEWDIEY
                     YHELLQVGGGKERMKKHWQNKGFSKPMTEEEIDALVKDMHKRKTALFVELIETGKLPL
                     RPGIHRFMKEAMDAGLKLAICTTSNEQAAKAITEGPLKDIKFDVVLAGDVVEKKKPDP
                     AIYNLALEKLGLKPDEVMVVEDSKNGLAAAKGAGAKVVVTTNHYTEKEDVSAGDVIVS
                     CLGDPDGEKAEMRKGDIKGFDGIVHVKQLMELFG"
     gene            complement(1700..>2167)
                     /locus_tag="PKI33_11580"
     CDS             complement(1700..>2167)
                     /locus_tag="PKI33_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM37698.1"
                     /translation="VLITRTLSKAYGLAGMRFGYTIGHEDVIKQIAGSLLPWNVGTIP
                     MWAALAAFEDKEGLAERVKFNNDAVDYISEALSVIPGFYVMQSKANYILFDCGETGKT
                     GKQVLEYAETKGIILRGESKKYGSDGWFRVTIGSKEENELFVNTVLEFFGMKK"
BASE COUNT          525 a          548 c          538 g          556 t
ORIGIN      
        1 ccataagcgg gagaagtcca aaggtacagg gctgaaggcg aagagggtga tgatcaagat
       61 cacggtcaga ccgaggatga cgtacccgct ccagtcggtg agtttggtcg tgatatcaat
      121 gtagtagtgg ttcaagatcg cctgagttcc caatgcgatg atgaggaaga tggtttggtg
      181 tgtcgtgccc caagtggaga catccacacc aagagctgtg ccgaacaaca ggtctctgaa
      241 gaagacggtg tagagcaaca catctacgga ggaaacgacg cagatcaaac caatgaggtt
      301 aaaccaggca gttgcccaac cccagaattt cccgccgagg tgtgagctcc agtgatagag
      361 accaccggca gtggggaagg aggaggcgat ctgtcccata ccaacggcta cgctcatggc
      421 gagcacgcca ccgaccagcc agatcaaaaa tgcaccgccg gatccaagcg cgttgaatgc
      481 agcagggaat gctgagatac caccagccaa aatacaaatg atagaaaatg aaattgcgaa
      541 gttcgagaac ccgctcatgc ggcgggacag ttcctgcgca taccccaatt tatgcaggac
      601 ttgcttgtcg tgcgcttgac ttttttcagc cattttcgta attctccttg actatgagtt
      661 ggttttgtag atcaagaaag caaacttacg agcagcacat tattattcac ctgattgaca
      721 cctcctcctc ccgcgggagt tctcctctga aattatatgt tgaaaacact ggctgttaat
      781 aaaaggtacg actaataatt tatgttaaca atcattctcg ccaggtttgt ccataaaagc
      841 acaagggtgc atggcgtatt accatgcacc cttgtgggtt aaccaaatag ctccatcagt
      901 tgtttgacgt ggacgatacc gtcaaagcct ttgatatcgc ctttgcgcat ctcagctttt
      961 tcaccgtctg ggtcgcctag gcaggatacg atcacatctc ctgcgctaac atcttccttt
     1021 tcggtgtagt gattggtggt cacaacaact ttcgcaccag cgccttttgc cgcggcaaga
     1081 ccattcttcg agtcttccac aaccatcact tcatcaggct tgagcccaag tttctcaagc
     1141 gcaaggttgt aaatcgctgg gtcgggtttc ttcttttcaa ccacatcacc agccaacaca
     1201 acatcgaact tgatatcttt gagcggacct tcggtgatcg ctttggctgc ttgttcattc
     1261 gatgtggtac agatggcgag tttgagacct gcatccatcg cctctttcat gaaccgatga
     1321 atgcccgggc gaaggggcag tttgcccgtc tcgatcagtt ccacgaacag agccgtcttg
     1381 cgtttgtgca tatcttttac caatgcatcg atctcttctt cggtcattgg cttggagaag
     1441 cccttgtttt gccaatgctt cttcatgcgt tccttgccgc cgccgacttg gaggagctcg
     1501 tggtagtact cgatatccca ttcatcggtg aagccgaatt ctttgaaggt catgttgaaa
     1561 gacacgcggt gaccgtcgcg ctcggtgtcg atgatgacac cgtcttggtc gaagaagact
     1621 gctttaattt tggacatgag ttcctctttt caataggcat gacaggtttt tgaaacctgt
     1681 catgccttgt tgtgaataac tatttcttca ttccaaagaa ttcaagcacg gtgttgacga
     1741 agagttcatt ctcttccttg gaaccaatgg tcacgcggaa ccaaccgtcg gagccgtact
     1801 tcttgctttc gccacgcagg atgatgccct tggtttcagc gtattcgagc acctgcttgc
     1861 cggtcttgcc agtttcgccg cagtcgaaca aaatgtaatt ggctttggat tgcatcacgt
     1921 agaagccagg gatgacactc aaagcttcgc tgatgtaatc taccgcatca ttgttgaact
     1981 tgacgcgttc tgccagacct tctttgtctt caaaagcggc aagcgcagcc cacatgggga
     2041 tagtaccgac attccacggc aggagggagc cggcgatctg cttgatcaca tcttcgtgac
     2101 cgatggtgta cccgaagcgc atacccgcca agccataggc tttggagagt gtgcgggtga
     2161 tgagcac
//
LOCUS       DAOREF010000576         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39439_length_2165_cov_3.528910, whole genome shotgun sequence.
ACCESSION   DAOREF010000576 DAOREF010000000
VERSION     DAOREF010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39439_length_2165_cov_3.528910"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKI33_11585"
     CDS             complement(<1..742)
                     /locus_tag="PKI33_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37699.1"
                     /translation="MKKISLLLLALVLVACAPQGVQVPQSPVLKFFEKKSGQIAYIGI
                     DGNIYLTDQGATSTTQLTKDVTPDTQGTIAYQLPTWSKTGDQVAFIRLEQTGANALTA
                     EILVASTEDETAHSVYTSQSEYPFYLYWAPDGKTLTALTTTATQGSMALQSIPLDGSE
                     PRIIDTGSPFYWSWAPDSKTMIVHKNGGNPNAFNQLSFLKVDNTVSEFVMDVLPASFQ
                     SPAWSPDGAHILLTTLSAADKPQLVLADS"
     gene            complement(739..2010)
                     /locus_tag="PKI33_11590"
     CDS             complement(739..2010)
                     /locus_tag="PKI33_11590"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNM37700.1"
                     /translation="MSRKKNAMAYGFTALLVIQLACSSLSPIADEPVEVTVPLGPQVT
                     ETEQQTAVLESMLSQIDANYIYQKNSSVDLKALHTKYQAKIDKGLTSVEFNELMKQLE
                     AEFPTGDIVYATREERIENDTAASLVGYGGIGAFVNFQAEDEPHVVILDIIPGSPAEK
                     AGLKAHDSIYAVNGDPVLLEEGADVVQRIRGEAGTKVVLTVKTPGKSERDVEVTRAQI
                     TGTRGVIAEEIPNTDIGYIRFPTVGSATNASEVLDAMETFAKNSNLKGIVLDLRISGG
                     NSNFPLEDMLTLFLNDLSIDVYSLDKTNSFPIEGQDISGSQTIPLAVLVGENTNGTVE
                     IFAAAIQEKGRGIVIGSNSAGGIEALSSFALPNGGQILIASASFRVGENERLGIDGVI
                     PEVRVEAQWDDIIASEDPVIQQAIQSFEVQE"
BASE COUNT          510 a          526 c          523 g          606 t
ORIGIN      
        1 tggagtctgc caagaccagc tgtggcttgt ccgctgcgga gagcgttgtc agtaagatgt
       61 gggctccgtc tggcgaccag gctggggatt ggaaagaagc gggcaacaca tccataacga
      121 attcactgac cgtattatcc actttcaaga aggagagttg gttgaaggcg ttggggttgc
      181 cgccattttt atgaacaatc atagttttac tatctggggc ccaagaccaa taaaacggtg
      241 agcctgtgtc aatgatgcgc ggttcacttc catctagcgg aatgctttgt agcgccatac
      301 ttccttgtgt agccgttgtg gttaacgctg taagcgtttt gccgtctggc gcccaatata
      361 aataaaacgg atattcactc tgactagtgt agacgctgtg tgcagtttca tcctctgtac
      421 tagcaaccag tatctctgca gttaatgcgt tggctcctgt ttgctcaagg cgaatgaacg
      481 cgacttggtc accggttttc gaccaagtgg gtagttgata agcaatggtg ccttgtgtat
      541 ctggcgttac atctttagtg agttgtgtgg tgctggttgc gccttggtcg gtgagataga
      601 tgttcccgtc aatgccaata taagcgatct gaccagattt cttctcgaaa aattttaaga
      661 ccgggctttg cggcacttga accccttgcg gagcacaggc aaccaagacc aatgcaagca
      721 ataacaagga tatctttttc attcctgcac ctcaaaggat tggatggctt gttggatgac
      781 agggtcttcg ctggcgataa tgtcatccca ctgcgcttca acccggacct cgggaatgac
      841 tccatcaata cccaagcgtt cattttcgcc gacgcggaag gacgcgctcg caatgaggat
      901 ttgaccgcca tttggcaggg caaatgaact taatgcttca atgccaccag cagaattcga
      961 cccaatgacg atgcctctcc ctttttcctg aatagcggcg gcaaaaattt ctacggttcc
     1021 gtttgtattt tcaccgacca ataccgcaag tggaattgtc tgtgaaccag agatatcctg
     1081 cccttcaatc gggaaggagt tggttttgtc gagggaatac acgtcgatac tcaaatcgtt
     1141 gagaaagaga gttagcatat cctctaaggg aaagttcgaa ttcccgcctg aaatacgtag
     1201 atcaagaaca atgcctttga ggttgctgtt cttggcgaag gtttccattg catccaatac
     1261 ttcgctggcg ttggtggcgc tcccaactgt ggggaagcgg atatagccaa tatctgtgtt
     1321 tggtatctct tctgcgatca ctccgcgtgt gcccgtgatc tgtgcgcgtg taacttccac
     1381 atcacgttca cttttccccg gcgttttgac ggtgagcaca acctttgtcc ctgcttcgcc
     1441 gcggatgcgt tgcacgacat ctgcaccttc ttccaaaagc acagggtcac catttacagc
     1501 gtagatactg tcgtgtgctt tcaaaccggc tttttcagca ggagagccgg ggatgatgtc
     1561 gagaatcaca acgtggggtt catcttccgc ttggaagttg acgaaagcgc caataccgcc
     1621 ataccctaca aggctggcgg ctgtatcgtt ctcaattctt tcttcccgag ttgcgtaaac
     1681 aatatcccca gttgggaatt cagcttcaag ctgtttcatc agttcgttga attcaacaga
     1741 cgttaaacct ttatcgatct tcgcctgata ttttgtgtgc agagctttta aatccacgct
     1801 actgtttttc tggtaaatgt aattcgcatc gatctgtgaa agcatgcttt caaggacggc
     1861 agtctgttgc tctgtctctg tcacttgcgg tcccaaaggc actgtcacct ctacaggttc
     1921 atctgcgatg ggagataaac tggagcaagc caattgaatc acgagcaatg ctgtaaaccc
     1981 atatgccatt gcgttctttt ttctactcat ctaaagctcc ttccggcttg attaagacgg
     2041 taatacagat gccccctctt acgactgcta tgaaaccttc ttgatattct tgggattaaa
     2101 gaccgagatt tcggcaactc ttaagatggc ttgcgcttca gagtcgagcc gcacccaata
     2161 aaaac
//
LOCUS       DAOREF010000577         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39468_length_2164_cov_5.405880, whole genome shotgun sequence.
ACCESSION   DAOREF010000577 DAOREF010000000
VERSION     DAOREF010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39468_length_2164_cov_5.405880"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKI33_11595"
     CDS             complement(<1..1042)
                     /locus_tag="PKI33_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNM37701.1"
                     /translation="MELGIVRKIDIDSEMQQAYLDYAMSVIVSRALPDARDGMKPVQR
                     RILYAMYDMGIRSDSPYKKSARIVGEVLGKYHPHGDSSVYEAMARLAQDFSMRTMLVD
                     GQGNFGSVDGDPPAAMRYTEARLTSAALDILNDLNKNTVDFTANFDDTLEEPSVLPAA
                     IPNLLVNGATGIAVGMATSIPPHNLSEVVDALVFMLERWEKLDDIDVEQLMKFVKGPD
                     FPTGGLIIEEKGDEGIESAYGSGRGRITVQAKAHIEEMERGKSRIIVSELPYMVNKAS
                     LIERIAELARDGHLEGLSDLRDESDRQGMRIVLELTKNADPNFVLRDLYKRTPMQTTF
                     SINLLALVDGEPR"
     gene            1196..>2164
                     /locus_tag="PKI33_11600"
     CDS             1196..>2164
                     /locus_tag="PKI33_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNM37702.1"
                     /translation="MTFITDSQTAPAPYKNDPRIEKDENGTWHVRGYTEARDLLKEDL
                     IQDGFNAEELRKAGFDGVLYMSGDPHRQMRAAIAKYFSPTTVQNTHMTVIEKTADDLL
                     SRLQARKRLNLKELTLEMATVIATAVVGLDPTPGLIQRLNSMLHAPKMSENKLIASLQ
                     HYEGHYRRFAFFFADVLPAIRRKRKNPSNDVISYMISKNKKPLEIWVECIVYGAAGMA
                     TTQEFILVVLLHCMKDAKLREIMTSGDATARYNALNEILRLEPVIGKIYRKPSVPVHV
                     SSEGKEYTIPAGERIAFHVYDVNADAKAVGETPEKLNPHRELEKGVY"
BASE COUNT          568 a          587 c          493 g          516 t
ORIGIN      
        1 tgcgcggttc accgtccacc aatgccagca agttgatgct gaaagtggtc tgcattggag
       61 tgcgcttgta gaggtcacgc aacacaaagt tcgggtctgc gttcttggtt agttcgagga
      121 caatgcgcat accttgacga tccgactcat cgcgcaagtc cgatagtcct tcgagatgac
      181 catcacgtgc caactcggcg atgcgttcga tcaaactcgc cttattgacc atgtatggca
      241 gttcagagac gatgatacgg ctcttgccgc gttccatctc ttcgatgtgt gccttggctt
      301 gcaccgtaat tcgtccacgt ccagaaccgt aagcagactc aatgccttca tcgccttttt
      361 cttcaatgat gagaccgccg gttgggaagt ctggtccctt tacgaacttc atcaactgct
      421 caacatcaat gtcgtcgagc ttctcccagc gttcgagcat gaatacgagg gcgtccacaa
      481 cttcacttaa gttatgaggc gggatggatg tcgccatacc gacggcgata cccgtcgctc
      541 cattcacgag cagattcgga atcgcagctg gaagcacact cggttcttca agtgtgtcat
      601 cgaagttggc agtgaaatcg actgtgtttt tgttgagatc attcagaata tcaagcgcgg
      661 cggatgtaag tctcgcttcg gtataacgca tcgcagcggg tgggtcacca tccaccgaac
      721 cgaagtttcc ctgaccatct accaacatgg tgcgcatcga gaaatcctgc gccaagcgag
      781 ccatcgcttc atacacagac gagtcaccat gtgggtgata tttacccaac acttcaccca
      841 caattctcgc agacttttta tacggcgagt cactgcgaat gcccatgtcg tacatggcat
      901 ataaaatacg acgctgcacc ggcttcatac catcgcgggc atcaggcaac gcgcgagaca
      961 caatgacaga catggcgtaa tcgagatacg cctgctgcat ttcgctatcg atatctattt
     1021 ttcgtacgat accaagttcc atgaaagttc ctttaaccac ataggctcaa aaattaaaaa
     1081 catcctgcct attatggtga attaatgttc tattgcgggg cttatcttac gggcaaacaa
     1141 acgatgcgtc aagtgtactg tataatgtgc atatcaactt tagagagagg catacatgac
     1201 ctttattact gattcacaga ccgcccctgc tccatacaaa aatgatccac gcattgaaaa
     1261 agacgaaaac ggaacctggc atgtgcgcgg atacaccgaa gcccgtgacc ttctcaaaga
     1321 ggatctgatt caagacggct tcaacgctga agagttacgc aaagccgggt ttgatggcgt
     1381 gctctacatg agcggcgacc cgcaccgcca aatgcgtgct gccattgcca aatatttcag
     1441 ccccaccact gttcaaaata cacacatgac cgtcatcgaa aaaacagccg atgacttgct
     1501 ctcacgttta caggcacgca aacgtctcaa cctcaaagag ttgactcttg aaatggcgac
     1561 cgtgatcgcc accgccgtag ttgggcttga tcccacaccc ggcttgattc aacgcctcaa
     1621 ctcaatgttg catgccccta agatgtctga aaacaaactc atcgccagct tgcagcatta
     1681 tgaaggtcat tatcgccgct tcgccttctt ctttgcagac gtcctgcctg ccattcgccg
     1741 caaacgcaag aaccccagca atgacgtcat ctcctatatg atctccaaga acaaaaagcc
     1801 gctagagatc tgggtggagt gtatcgtcta cggtgcagca ggcatggcaa ccacacaaga
     1861 atttattttg gtggtcttgt tgcactgcat gaaagatgcc aagctacgcg agatcatgac
     1921 ctctggcgac gccaccgcac gctacaatgc cttgaatgaa atcttgcgtc ttgaacccgt
     1981 catcggcaag atataccgca aaccttctgt gccagtccat gtgagcagcg aaggcaagga
     2041 atacaccatt cctgctggag agcgcattgc ttttcacgtc tatgatgtca atgccgatgc
     2101 caaagctgta ggtgaaaccc cagagaaatt aaatccacac cgtgaattgg aaaaaggggt
     2161 ctat
//
LOCUS       DAOREF010000578         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39470_length_2164_cov_3.979611, whole genome shotgun sequence.
ACCESSION   DAOREF010000578 DAOREF010000000
VERSION     DAOREF010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39470_length_2164_cov_3.979611"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKI33_11605"
     CDS             <1..372
                     /locus_tag="PKI33_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNM37703.1"
                     /translation="EILLKPGKLTEEEWVIMRKHPLYAQMLIAPINYLEPAMTIPLYH
                     HERWDGSGYPFGLHGTEIPLEARVFAIIDVWDALRSDRPYRKALLDEEALTYIQAQAD
                     KQFDPEVVRVFAEMVRDNSLK"
     gene            complement(388..768)
                     /locus_tag="PKI33_11610"
     CDS             complement(388..768)
                     /locus_tag="PKI33_11610"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 family protein"
                     /protein_id="HNM37704.1"
                     /translation="MNKKNILITGDVLAFAILNIIGFATHKETGVSFLPRMAVAFFPI
                     VFAWFVLAPWFGLFDETVINNSKNLLRIPLVFLFVAPLAVILRGALLNAPALPLFALV
                     FGSTNAFGMLVWRWLYLVLFAKKA"
     gene            complement(761..2125)
                     /gene="der"
                     /locus_tag="PKI33_11615"
     CDS             complement(761..2125)
                     /gene="der"
                     /locus_tag="PKI33_11615"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNM37705.1"
                     /translation="MKPIVALVGRPNVGKSTLFNRLVGERMAIVDDTPGTTRDRLFGD
                     AEWNARPFTVIDTGGIDPTHGGRTPLSVGSADFIGDIRRQAQAAIQEADAILFVVDGE
                     TGVTEPDREVADILRRMQKKQPDGSFLPPIILVVNKCESRERRDSVGEFYELGLGEPF
                     AISAIHGTNTGDLLDALVSSFPTQNEDEEDNNIKIALVGKPNAGKSSLLNKLVGEERV
                     IVSPIAGTTRDAIDMELEFGGLPITLIDTAGIRRRGKIEKGVEEYSVIRSFKAIERAD
                     VALLMIDATTGITSQDAHIAGFIKDEWKSCVVLVNKWDAIEKDGTTMDAYTEKILNDL
                     NFVSYVPILYISAKTGQRVDQVLPLALRVQEERLARLTTSKINEIIHAAQDAHPHPTH
                     AGRALKMYYGTQVRSDPPTFMIYVNEPSLMHFTYMRYLENQIRAEYGFLGTPIRIVLK
                     GRHE"
BASE COUNT          574 a          486 c          562 g          542 t
ORIGIN      
        1 gagattttgc tcaaacccgg aaaactgact gaagaagaat gggtcatcat gcgaaagcat
       61 ccgctttatg ctcaaatgct aattgccccc attaattatc ttgaacccgc catgacaatt
      121 ccgctatatc accatgagcg ctgggatggg agtggatatc cgtttgggtt gcatggaaca
      181 gaaattcccc ttgaagccag agtgttcgca atcattgatg tgtgggatgc cctccgttcg
      241 gatcgcccat atcgtaaagc acttttagat gaagaggcgt tgacttatat tcaagctcag
      301 gcggataaac aattcgaccc ggaggttgtc cgggtctttg ctgaaatggt tagagataat
      361 agtttgaaat gatgtgaatt ttagatgtta cgcttttttc gcaaacagaa cgagatataa
      421 ccaacgccac accaacatac caaaggcatt cgtactgcca aagactaacg caaaaagcgg
      481 caacgctggc gcgttgagca atgctccgcg caagatcact gccagagggg ctacaaacag
      541 aaacaccagt gggattctca aaagattttt cgaattattg atgacagttt catcgaacaa
      601 accaaaccat ggggcgagaa caaaccatgc aaaaacaatt ggaaaaaagg caactgccat
      661 acgcggcagg aaagaaacgc cagtctcttt gtgagttgca aagccaataa tatttagaat
      721 cgcaaaagcg aggacgtcgc ctgtaatgag gatgtttttc ttattcatgt ctgcccttga
      781 ggacaatacg aatgggggtt cctaagaaac catactcagc gcggatttga ttctctaaat
      841 aacgcatgta cgtgaagtgc atcaacgaag gttcattgac atagatcatg aaggtgggcg
      901 gatctgagcg gacttgtgtg ccgtaataca tcttaagagc acgcccggca tgcgtagggt
      961 gcggatgggc atcttgcgcg gcgtgaatga tttcattgat cttcgaagtc gtcaggcggg
     1021 cgaggcgttc ttcttggaca cgcaatgcca agggtaagac ttgatccacg cgctgaccgg
     1081 tcttggcgga gatataaagg atgggaacgt acgaaacaaa attcaggtcg ttcagaattt
     1141 tctctgtgta ggcatccatc gtggtgccat ctttttcaat ggcatcccat ttgttgacca
     1201 aaaccacaca agatttccac tcgtctttga taaaaccagc aatgtgggca tcttgtgagg
     1261 tgataccggt ggtggcatcg atcatcagta acgcaacgtc tgcgcgttca atggctttga
     1321 aagaacggat aacgctgtac tcttccacgc ctttttcaat cttgccacgg cgacggatac
     1381 ccgcagtatc aatgagagtg atgggcaacc cgccaaattc taattccatg tcgatggcat
     1441 cacgggtggt ccctgcaatt gggctgacga tcacacgttc ttcgccaacg agtttattga
     1501 gcaggcttga tttcccggca ttgggtttgc ccaccaaggc gatcttgata ttgttatctt
     1561 cttcatcttc attttgagtc gggaacgaag agacgagtgc atcgagtaag tcaccggtgt
     1621 tggtgccgtg aatggcagaa atggcaaagg gttcccctaa acctagctca taaaattcac
     1681 ctacagaatc acggcgttct cttgattcac atttgtttac gactaatatg atcggaggca
     1741 gaaaagaacc atcgggctgt ttcttttgca tgcggcgcaa aatatctgcc acttcacggt
     1801 ctggctcagt gacgccggtt tcgccatcga cgacaaagag gatggcgtcg gcttcttgaa
     1861 tggcagcttg ggcttgtcgg cggatgtctc cgataaaatc ggcagaacca actgagagcg
     1921 gagtccgacc gccgtgagtc gggtctatac cgcctgtatc aataacagtg aagggacggg
     1981 cgttccactc tgcatcaccg aaaaggcggt cacgggtggt gccgggggta tcgtcaacga
     2041 ttgccatgcg ttcgccaacg aggcgattga acagggtgga tttaccgaca ttgggtcggc
     2101 cgactagtgc aacaatgggt ttcatagggt ttattttact ttatggttta ggggttggtg
     2161 tggg
//
LOCUS       DAOREF010000579         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39695_length_2158_cov_4.932002, whole genome shotgun sequence.
ACCESSION   DAOREF010000579 DAOREF010000000
VERSION     DAOREF010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39695_length_2158_cov_4.932002"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1626)
                     /locus_tag="PKI33_11620"
     CDS             complement(121..1626)
                     /locus_tag="PKI33_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNM37706.1"
                     /translation="MAIIKEFIRKIHAQQEKHKTWMDGLFLVLCVLYILSGIPLVPFH
                     GDESAYIILSEDYDRVFKKHEVEKVLFDPDGPKQYLRLSTGAILSFSIGFARDITNNE
                     DPINKWLWGASWDENLAKGNLPAPSLLNVARFSSAIMGALGTVLFFVLLRYLYPSRLV
                     AWLATLAFVTQGSLLVSIRRAMQEGPKFLFLILTTLLAFLIIQNLQMQKLRQALYFAL
                     GIASGLTLAAKQDTAPVLVALYLTIAFIPVWNRTSFRAVWHTLLYLFAATLLAYATFL
                     AFMPVFWQWWESVFVLTGLSVALFQIPFIHETRSKVLASAGLALVVVMTFVSPALWGK
                     LHIPLSSMAELRQSIVGSQVEVSNGQDVLKADSAMGRLNFLLENTISSKVMYMEVDSF
                     NVPPMQASIKAYEASFLSGRTGSFVFDGLIFLLVLLGMWFLLRNFNSTSLLVYLLFFV
                     TGTFLLVAIPLAWQRYFIIMQLPYMLFAGAGVYQLEMWLNPLFLEKKTASA"
     gene            1646..2149
                     /locus_tag="PKI33_11625"
     CDS             1646..2149
                     /locus_tag="PKI33_11625"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNM37707.1"
                     /translation="MEINQPVPDFELPDLNGARHRLSNYRGRIVIVNFWSCECPHSER
                     TDKAILSMFVQWQDDVTMLTIASNRSETVEALKEAAESRRLPNVLHDADCLVANLFDA
                     QTTPHVFVIDRDGILRYRGAVDDVTFRQRKPTRFFLDEAVEALLSGQFPTLTESPAYG
                     CAIVREV"
BASE COUNT          647 a          514 c          478 g          519 t
ORIGIN      
        1 ttcttcaaga tgatggttga acaaggtatg ggcgagttgg acaatgccgc agtggtaggt
       61 gtgattgaga aattggctgg ggttgagata ccctaataaa agcttttaca aaaacagcct
      121 ctacgcagag gctgttttct tttccaggaa taagggattc aaccacatct ctagttgata
      181 tacgccagcg cccgcaaaaa gcatataagg aagttgcata atgatgaaat atctttgcca
      241 tgccaaagga attgcgacca gtaaaaaagt gcctgtaacg aaaaacaaga ggtaaaccag
      301 aagactggtc gaattgaaat tgcgcaaaag aaaccacatt cccaaaagca ccagcaagaa
      361 gataagtccg tcgaacacga aagagccggt gcgcccgctc aggaatgaag cttcatacgc
      421 ctttatgctt gcttgcatag gcggcacatt aaaactatcc acttccatgt acatcacctt
      481 ggatgaaatg gtattttcaa gaaggaaatt cagcctaccc attgcagaat ccgccttaag
      541 aacgtcctgc ccgtttgaaa cctccacttg agaccctaca atcgattggc gtaattctgc
      601 catactgctc aatgggatat gtaatttccc ccataatgcc ggtgatacaa atgtcatcac
      661 aaccaccaat gccagcccgg cacttgccag cactttcgaa cgcgtctcat ggataaaagg
      721 gatttgaaat aaagctacag aaagaccagt taatacaaaa acactttccc accattgcca
      781 aaaaacgggc ataaaagcaa gaaaggttgc atatgcaagc agagtggcag cgaaaagata
      841 taacaaggta tgccaaacag cccggaaaga tgttctgttc caaactggta tgaacgcaat
      901 cgtcaaatag agcgccacca aaaccggagc tgtatcttgc ttggcggcaa gggttaatcc
      961 gctggcgatg cctaaagcaa agtataaggc ttggcgcagt ttctgcattt gaagattctg
     1021 aatgatcaaa aaagcaagta aggttgtcag gatcaaaaac aaaaacttcg gaccttcttg
     1081 cattgcccgc cgaatgctaa ccaaaagact gccttgcgtt acaaaggcaa gggttgccag
     1141 ccaggcaacc aagcgagacg gatacaaata acgcaaaagc acaaaaaaca gcacagttcc
     1201 caaagcgccc ataattgcag aactaaacct ggcaacattc aacaagcttg gtgcagggag
     1261 attccccttg gcaaggtttt catcccaaga tgccccccac aaccacttgt tgatcgggtc
     1321 ttcattgttg gtaatatctc ttgcgaagcc aattgaaaat gacaatattg cccccgtaga
     1381 taatcgcagg tattgtttgg gaccatctgg gtcgaacaac accttttcta cttcatgctt
     1441 cttaaagact ctatcgtaat cttcgctcaa aataatataa gcagattcat ccccatgaaa
     1501 tggcaccaaa ggaatcccgc taaggatata gagtacacat aacaccaaaa acaaaccgtc
     1561 catccatgtt ttatgttttt cctgctgagc atggattttt cttataaatt cctttatgat
     1621 cgccatgttg aacattatat ctaaaatgga aattaaccaa cccgtccctg attttgaatt
     1681 gcccgacctt aatggcgccc gccatcgtct ttcgaattat cgcgggcgca ttgtaatcgt
     1741 caatttttgg tcgtgcgaat gtccgcattc cgagcggaca gataaagcga tactgtccat
     1801 gttcgtgcaa tggcaggatg atgtgacgat gttgactatc gcgtcgaatc gcagcgaaac
     1861 ggttgaggcg ttgaaagaag ctgctgaaag tcgccgcctg ccaaacgtct tgcatgatgc
     1921 ggattgcttg gtcgcaaatt tatttgacgc gcaaacgacg ccgcatgttt tcgtcatcga
     1981 ccgggatgga atcctgcgct atcgtggcgc ggtggatgat gtgaccttcc gccagcgcaa
     2041 gcccacccgc ttctttctgg acgaggcggt tgaggctttg ctctctgggc agttccccac
     2101 actgacggaa tctcctgctt acggctgcgc cattgttcgt gaagtttgat agaatcac
//
LOCUS       DAOREF010000580         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_39828_length_2155_cov_2.400000, whole genome shotgun sequence.
ACCESSION   DAOREF010000580 DAOREF010000000
VERSION     DAOREF010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39828_length_2155_cov_2.400000"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKI33_11630"
     CDS             <1..491
                     /locus_tag="PKI33_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37708.1"
                     /translation="SLFAGGMRWRVAYIGDESFFTHSPLVTIKDIYFFENFMVSPSAK
                     SVMLEARSVLIENYGDERNWTNSPVYFGPRIEFAYAVFGITSPVNMPIWWHPNNSYPP
                     EYEAEYVGAFIDYGFENAIFMKQPGGRDPDYGFLPEGIISELETNYNRTDYSTIVVFH
                     TK"
     gene            516..>2155
                     /locus_tag="PKI33_11635"
     CDS             516..>2155
                     /locus_tag="PKI33_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNM37709.1"
                     /translation="MRVSEYIANHLAELGVRHIFMLTGGGAMFLNDALGNQAGIQPIF
                     NHHEQASSIAAEGYARITNTPGVINVTTGPGGINALNGVFGAWVDSIPMLVLSGQVKR
                     ETLVSSYNIPGLRQIGDQEADIIGMVKGITKYAVLVTDPQTIRYHLERAWHLCKTGRP
                     GPCWLDIPIDVQSSQIDPASLKGYDPHEDDQQTHELNLSQVCAEIVKKIGMARRPVIL
                     AGKGIRLAGAIDSFERVRKKIGMPVAAAWTGIDLIAYDDPLYAGCSADVGTRAGNYAV
                     QNADLLLILGTRMSLRQLTYNWKAFARHAFKIHVDIDPAEFQKPMFTADLPLACDLKE
                     FLEELDRQIDAAKYKSIHDEWVQWCRERVRKYPTLQERHKEFKGEFINPYYFMDRVFD
                     LMKDDDIVVCGNGAANVVTFQAAKIKRGQRLFCNTGDASMGYDLPAALGASIAAPTHR
                     VLCMAGDGSLQLNIQELQTMKQLNLNVILFVLNNNGYLSIRLSQNNFFKRLVGESPAS
                     GVTFPDTLKLADAYGLPSMKIDAQNFESGLKQVLASSGP"
BASE COUNT          558 a          459 c          544 g          594 t
ORIGIN      
        1 tttcactttt tgctggcggg atgcgttggc gggtcgcata tattggggac gaatcttttt
       61 ttacacactc cccgcttgta accattaaag atatttattt ttttgagaat ttcatggtct
      121 caccatcagc aaaaagtgtg atgctagagg ctcggtcagt gttgatcgaa aattatggag
      181 atgagaggaa ctggacgaat tctcccgtct attttggtcc acgtatcgag tttgcgtatg
      241 cagtatttgg aataacttcg cctgttaata tgccgatctg gtggcatcca aacaacagct
      301 atccgccaga atacgaggcg gagtatgtcg gagcattcat cgactatggt tttgaaaatg
      361 cgatattcat gaagcaaccc ggcgggagag atcccgacta tggttttttg cctgaaggta
      421 ttatttctga attagaaacc aattacaatc gtaccgatta ttccactatt gtagtgttcc
      481 ataccaaata aactgagtga attgagtgag tacttatgag agtctctgaa tacattgcta
      541 accaccttgc agaacttggc gttcgccata tttttatgct tactggcgga ggggcaatgt
      601 tccttaatga cgcactgggt aatcaagctg ggatacagcc gattttcaat catcacgaac
      661 aggcaagttc cattgcggct gagggttacg cgcggattac gaacacacct ggagtcatta
      721 atgttacgac aggtcccggc ggcatcaatg ctttgaatgg cgtttttggc gcctgggtgg
      781 attcgatccc tatgttggtg ctttctggtc aagtcaaacg tgaaacactt gtttcttctt
      841 acaacatccc tggtttgcgg cagatcggtg atcaggaggc agatatcatc gggatggtca
      901 agggaataac gaaatacgcc gtgcttgtta ctgacccgca gacgattcga tatcacttgg
      961 agcgtgcatg gcatttgtgt aaaaccggca gaccaggtcc ttgctggttg gatataccca
     1021 ttgatgtcca atcaagtcag atcgacccgg cgtcattgaa gggatacgac ccacatgaag
     1081 atgatcaaca aacacatgaa ttaaaccttt cacaagtatg tgcagagatc gtgaagaaaa
     1141 tcggcatggc gcggcgacct gttattttgg caggcaaggg aatacggttg gctggtgcaa
     1201 ttgattcatt tgaacgagtg cgaaaaaaaa tagggatgcc tgttgctgca gcgtggacgg
     1261 ggatcgacct tatcgcttat gacgacccgc tttatgcggg gtgtagcgcc gatgtaggaa
     1321 cgcgggcggg aaactatgct gtacagaatg ccgacttgct tcttattctt ggaacacgta
     1381 tgagcctgcg tcagttgaca tataattgga aagcgtttgc ccgccacgct tttaagatcc
     1441 atgtggatat tgatccggct gaatttcaga aacccatgtt tacggcagac cttccactag
     1501 cctgtgattt gaaagagttc cttgaagaac ttgaccgaca gatcgatgca gctaaatata
     1561 aaagcatcca tgatgaatgg gttcagtggt gtagagaacg cgtccgtaag tatcctactt
     1621 tgcaggagcg gcacaaggaa ttcaaaggtg aattcattaa tccatattat tttatggacc
     1681 gtgtttttga cctgatgaaa gacgatgata ttgtagtgtg cgggaacggt gccgccaatg
     1741 ttgtgacctt ccaggcagcc aagatcaaac gtggacagcg gctcttttgc aatactgggg
     1801 atgcttcgat ggggtatgat cttcccgctg cccttggcgc ttccattgca gcgccaacac
     1861 atcgggtctt gtgcatggca ggcgacggca gtttgcagtt aaatattcaa gaattgcaaa
     1921 ccatgaagca attgaatttg aacgtcattc tttttgtgct gaacaacaat ggctaccttt
     1981 ccatccgcct ttcacaaaat aacttcttca agcgtctggt aggggagtct cctgcgagcg
     2041 gtgtgacctt cccagatacg ctcaaattag cggatgctta tgggcttcct tcaatgaaga
     2101 ttgacgctca aaattttgaa agtgggctca aacaagtgct cgcctcaagc ggacc
//
LOCUS       DAOREF010000581         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40012_length_2150_cov_2.830072, whole genome shotgun sequence.
ACCESSION   DAOREF010000581 DAOREF010000000
VERSION     DAOREF010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40012_length_2150_cov_2.830072"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PKI33_11640"
     CDS             complement(<1..1539)
                     /locus_tag="PKI33_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37710.1"
                     /translation="MEQNIKILFVDDDPEMRTLVSFILKRAGYEVELVESATEAFQYL
                     THSTPNLILSDVMMADSDGFTLLDTLRKQEATRHIPVILLTALAAPSDLVKGLSLGAD
                     DYIQKPFQSAELLARIQSKINRPPMPSELVPMDIRSGLVKPEQFEEEFKKEFFRSLRN
                     GSEGFIAFLSLSELAITRERVGMDIAPEIWKQAARSIAADLGPTDVIGLANGNLLGII
                     LPDMLEATAHKILVRLTRKIMNYTFVVGNERFRLTPSVGYANFLSAKNAADARNRALT
                     ALDNAIHHLDMEPRLYEPSMGSIEKKEKNSFFQSVAQVLEKLILPFQIGLTFLIGIIL
                     PYFIYRWMDFIGYDITPVAYMIVTISLVLTALLIWVEGFYALRKNPIPETPGAPYPPA
                     TAVIAAYLPNEGATIVDTIEAFLRLDYPAPLQIILAYNSPYEMPIERDLKEIAARDPR
                     FVPFRVSNSTSKAQNINTVIAKVTGEFIGIFDADHHPNADSFTRAWHWLSNGYSIVQG
                     HCAVR"
     gene            complement(1703..>2150)
                     /locus_tag="PKI33_11645"
     CDS             complement(1703..>2150)
                     /locus_tag="PKI33_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNM37711.1"
                     /translation="NFGAVPPHQTRHRIIIPISGVHQGSLAALRYARMLSDDVTAVHV
                     AIEPAEADKVKKKWELWGEGTRMVMLNSPYRLFLEPMLEYITEISERRQPGETLTIVV
                     PEFISNNMLGAALHTNTADLLRSQLKRQEGVVIINVPYQLREHVKN"
BASE COUNT          571 a          495 c          509 g          575 t
ORIGIN      
        1 gcggacagcg caatgcccct gcacaatgct gtatccgttc gaaagccagt gccatgcgcg
       61 ggtaaaacta tcagcattcg gatggtggtc tgcatcaaag atacctataa actcgccagt
      121 aaccttggca attacggtat tgatattttg agccttggat gtactattgc tgacccggaa
      181 cggtacaaaa cgcggatcgc gtgcggcaat ttctttcaag tccctttcga tgggcatctc
      241 gtatggactg ttataagcaa ggatgatctg caaaggcgca ggatagtcca acctcaaaaa
      301 cgcttctatt gtgtcgacaa tggttgcgcc ctcgttgggc aaataggcag caatgacagc
      361 ggtcgctggc gggtatggcg ccccaggcgt ttcagggatt ggattctttc tcagcgcgta
      421 aaatccttct acccagatca gaagcgctgt aagcaccaac gaaatggtca cgatcatata
      481 ggctacaggg gtaatgtcgt aaccgataaa gtccatccaa cgatagatga agtacgggag
      541 gataatgccg attagaaagg ttagcccaat ttgaaagggt aggatcagtt tctccaaaac
      601 ttgcgccaca gactgaaaga agctgttttt ctcttttttc tcaatagatc ccatagacgg
      661 ttcgtaaagg cgcggctcca tatccaagtg gtggatggca ttgtccaaag cagttaaggc
      721 gcggttccta gcgtcggctg cattttttgc agaaagaaaa tttgcatatc caaccgaagg
      781 cgttagacga aatcgctcat tcccaactac aaatgtatag ttcatgatct ttcgtgtgag
      841 cctgactaaa attttatgtg cagtggcttc cagcatatcc ggcaggatga tccccaagag
      901 attcccattt gccagaccga tcacatccgt aggacccagg tcagcggcga tggatcgagc
      961 tgcctgcttc catatttcag gtgcgatatc catgccgacg cgttcgcgag taatggcgag
     1021 ttcggataaa gataaaaacg cgataaaccc ttcagaacca ttgcgtaagg atcgaaaaaa
     1081 ctccttttta aattcttctt cgaattgttc tggtttcacc aacccactgc ggatgtccat
     1141 gggcaccaac tcgctaggca tgggcggacg attgatcttc gattgaattc ttgccaatag
     1201 ttcagccgat tggaatggtt tctgtatgta atcatctgcg cccaaggaca acccttttac
     1261 taggtcactg ggggcagcta gagcagtaag taatatcact ggaatatgcc tggttgcctc
     1321 ttgttttctg agtgtatcaa gcagagtaaa accatcggaa tctgccatca tgacatccga
     1381 caaaataagg ttaggggttg aatgggttag atactgaaat gcctcagtcg cactttcgac
     1441 caactccact tcgtagcctg ctcttttgag aatgaagctt acaagggttc gcatttccgg
     1501 atcatcgtct acaaatagga tttttatgtt ctgttccatt ttttagctcc ttgaaagata
     1561 atctttcaat ttcgcttaga aaatacccca aaactatgta ttaattccat tagggaaaac
     1621 ctgtatgaaa caagaaagac aaaaccctct atccattttg taaggaagag ggttttgtct
     1681 ttgcagtttt ttgtttaatt atttaattct tcacatgttc acggagctga tacggaacat
     1741 tgatgatgac gaccccttcc tgacgtttca actggctacg caacagatct gctgtgttgg
     1801 tgtgaagagc cgcacccaac atattgtttg aaatgaactc aggcaccaca atggtgaggg
     1861 tctcgccggg ttggcggcgt tcggagattt cggtgatgta ttccagcatc ggctcaagga
     1921 agaggcggta tggcgaattc agcattacca tgcgtgtccc ctctccccat agctcccatt
     1981 tctttttaac tttatccgct tcagcgggct caatggcaac atggacagca gtgacatcat
     2041 ccgaaagcat acgcgcgtat cgcagtgcag ccaatgagcc ttgatgaaca ccgctaatcg
     2101 gtatgatgat gcgatgacgg gtttgatgcg gcggaacagc accgaaatta
//
LOCUS       DAOREF010000582         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40080_length_2148_cov_4.399904, whole genome shotgun sequence.
ACCESSION   DAOREF010000582 DAOREF010000000
VERSION     DAOREF010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40080_length_2148_cov_4.399904"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="PKI33_11650"
     CDS             <1..1802
                     /locus_tag="PKI33_11650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNM37712.1"
                     /translation="WADGQFVSVSGTGYTPKGDFLVDGKVVDAKQYPAILSTLWLGVL
                     NNDAEIEITGETDSQKTYRIVGDPTEGSLLVAAAKAGAIHVEIDEAYPRENEVPFDSE
                     RKRMITIHDVSSPNPNDPSPFTNEKHKDWDVIAVKGAPDIVLTLCSKYQGMDDQPREL
                     TDEMRSKILGANDAMTKDALRVLGFAYRLEKNVPDNLESIKTEDLEKDLVFVGLMGMI
                     DPPRTEVKPALEKARHAGIRTVMITGDFPNTAKAIAESIGLLRPGKGVMTGAELDKID
                     DTQLKNIIEDTDVFARVSPEHKMRIVDALQANDEVVAMTGDGVNDAPAIKRADIGVAM
                     GITGTDVAKGTADMVLTDDNYASIVSAVEQGRIIYSNIRKFVFFLLSSNVAEIMIIFL
                     ATLAGLPAPLTAIQLLWLNLITDGAPALALAMEKGDPDIMDQKPRAKSEPIVNRSMGI
                     GIVVQTIMQTSAVLIAFVMGLAWHLESGAIIPSGANFLSHVLAHDWRGVDVQTAETMA
                     FATLSMAELFRAYTVRSERASVFKIGIFSNKYMQYAVGISMVLLLLVCAVPFLQPIFN
                     THFLSLREWGLVVGLSLMPATAEEVTKFFLRKG"
     gene            1897..>2148
                     /locus_tag="PKI33_11655"
     CDS             1897..>2148
                     /locus_tag="PKI33_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37713.1"
                     /translation="MKKEKRTLTLNEWIWLILGSAVILGSIVRLLPGVLAGFPLNDGG
                     MFLSMVKDLQTSHYVLPKFTTYNYLNIPFAYPPFGFYFAR"
BASE COUNT          565 a          513 c          498 g          572 t
ORIGIN      
        1 tctgggcaga cgggcagttt gtcagcgttt caggaaccgg atacacgccc aaaggtgatt
       61 ttctcgtaga cggcaaagtg gtggatgcaa agcaatatcc cgccattctt tccacactgt
      121 ggcttggggt cttgaacaat gatgctgaaa ttgagatcac aggcgagacc gactcacaga
      181 agacttaccg tattgtaggc gaccccaccg aaggctcttt gctggttgcg gcggcaaaag
      241 ctggtgctat tcacgttgaa attgacgaag cctatccgcg tgaaaatgaa gtcccatttg
      301 actcagaacg caaacgcatg atcaccattc atgatgtgag cagccctaat cccaatgatc
      361 catctccgtt cactaatgaa aagcataaag attgggatgt cattgccgta aaaggtgctc
      421 ctgatatcgt gttgacactc tgctcaaaat atcaaggcat ggatgatcaa ccacgtgaat
      481 taacggatga aatgcgtagc aaaattcttg gcgcaaatga tgccatgacc aaagacgctc
      541 tacgtgtgct tggctttgcc tatcgcttgg aaaagaacgt acccgataac ctagaaagta
      601 ttaagaccga agaccttgaa aaagatttgg tctttgttgg tttgatgggc atgatcgacc
      661 caccgcgcac cgaagtaaaa cctgcactcg aaaaggcacg ccatgcgggc atccgcacag
      721 tgatgatcac aggcgacttc ccaaacactg ccaaggcgat cgcagagtcg attgggttgt
      781 tgcgacccgg caaaggcgtc atgaccggag ccgagttgga caaaatcgac gatactcagc
      841 tcaagaacat catcgaagat acagacgtct tcgctcgcgt ctctcctgaa cacaaaatgc
      901 gtattgtgga tgcgttacag gcgaatgatg aagttgttgc catgactggc gatggcgtga
      961 atgacgcacc tgccatcaaa cgcgcagaca ttggtgtggc aatgggtatc actggcaccg
     1021 acgtcgccaa aggcactgct gatatggttc tcacagacga taactatgcc agcatcgtct
     1081 ctgccgtaga acagggacgt atcatctact caaacattcg caagttcgtg ttcttcctgc
     1141 tttcgtccaa tgtggctgag atcatgatca tcttccttgc gactctcgcg ggtctacctg
     1201 cgcctttgac tgctattcaa ctcctgtggc ttaaccttat tacagatggt gctcccgcat
     1261 tggcgcttgc catggaaaaa ggcgaccccg atattatgga tcaaaagccg cgtgctaaat
     1321 ctgagcccat cgtcaatcgt tccatgggta ttggtatcgt tgtccaaacc atcatgcaaa
     1381 catctgctgt attgatcgcc tttgttatgg ggttggcgtg gcaccttgaa tcaggagcga
     1441 taatcccatc tggagcaaat ttcctgtctc atgtccttgc ccatgactgg cgcggcgtgg
     1501 atgtgcaaac agcagaaaca atggcgtttg cgaccctttc aatggcagaa ttgttccgtg
     1561 cgtacacagt ccgttcagag cgtgcttctg ttttcaaaat cggcatcttc tcaaataaat
     1621 atatgcaata tgctgtaggc atctccatgg tgttgttact cttggtttgt gccgtaccgt
     1681 ttttgcaacc tattttcaat acacacttcc tgagcctgcg tgaatgggga ctggtggttg
     1741 gattgtcact tatgccagct accgcagaag aagttacaaa atttttctta agaaaaggct
     1801 agcactaaaa gagccgccct tcgtaaaacg aagggcggct cttttttatg acatagtgta
     1861 ttattcatta cattaataac aatctactta aacattatga aaaaagaaaa acgcacccta
     1921 accctcaatg aatggatctg gttaattctt ggttcagcag tgattcttgg aagcattgtt
     1981 cgtcttttgc ctggcgtctt agcaggcttt ccacttaatg atggtggcat gtttctaagc
     2041 atggttaaag atctacagac cagtcactat gttcttccca aattcactac atacaattat
     2101 ctaaacatac cttttgcata cccacctttt gggttttact ttgcgagg
//
LOCUS       DAOREF010000583         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40158_length_2146_cov_2.670493, whole genome shotgun sequence.
ACCESSION   DAOREF010000583 DAOREF010000000
VERSION     DAOREF010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40158_length_2146_cov_2.670493"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKI33_11660"
     CDS             <1..678
                     /locus_tag="PKI33_11660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM37714.1"
                     /translation="TFFTSDANVTKDKAVAYGMNPEKTIVFPWGVDLDHFTKKEEIDS
                     KEGFVLFCNRSWETRYGVDVLARAFVKVASQREDIRLLLLGGGSQGAYLRKIFQSGGV
                     DEYVTYGGQISQTDLPRWYHMADLYISPSHVDGSSVSLMEALACGLPCLVSDIPANKE
                     WVFENQNGWLFRDGDVDHLAEKILAAMNQREKLPEIGKASRSEAEKRADWKKNAAALM
                     EVYRSLK"
     gene            787..1557
                     /locus_tag="PKI33_11665"
     CDS             787..1557
                     /locus_tag="PKI33_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNM37715.1"
                     /translation="MLSKKELLDGFGQLNLKKGETIIVHTSYKSLGGVEGGADTVIDV
                     MSELVGKDGTVLFPAFNFQSWTESHYFDVKETPSKMGAITEQARLRPNALRTPHPIYS
                     FSALGARAEEFSKTEDVEAYGPNSAFALFHKINGTIVSIGLDFNSTFSMHHYIEYNVG
                     CNYRRIKEFSGIYVGYDRVPKLKMHSMFVRNDDTVKTYINPGMNELLEAGVIKEVQVG
                     AAKIHFATANDFYDNMSVIVREHPEKLHIIEEPKYRKF"
     gene            1656..>2146
                     /locus_tag="PKI33_11670"
     CDS             1656..>2146
                     /locus_tag="PKI33_11670"
                     /inference="COORDINATES: protein motif:HMM:NF021459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2172 domain-containing protein"
                     /protein_id="HNM37716.1"
                     /translation="MKPYNSLKSILAEFFPLHRTLVSDDHDKTLEIVGSYMPESSNYT
                     VETYAPLKQVWTWKVPERYVVHEAYLEIEGGERIVDFKDNPLHIVSYSLPVDKVLNFD
                     ELQPHLYFNEKLPHTVPWVFKYYERSWGFCLPKNTFDKLPRDKKYRAIINCEFIADPA
                     QGF"
BASE COUNT          556 a          464 c          576 g          550 t
ORIGIN      
        1 acattcttca ctagcgatgc taatgtgact aaagataaag ccgttgcgta tggcatgaat
       61 ccagaaaaga ccatcgtctt cccttggggt gtggacttgg atcatttcac aaagaaagaa
      121 gaaatagata gtaaagaagg tttcgttcta ttttgcaacc gctcctggga gacacgttac
      181 ggggtggatg tgctggcgcg cgcgttcgtt aaagtggcaa gccagaggga ggatatacgg
      241 ttattgcttt taggcggcgg gtcgcaggga gcctatcttc gcaagatttt tcaaagcggc
      301 ggtgtggatg agtacgtcac ctatggcggg cagatctcgc agacggatct tccgcgctgg
      361 tatcatatgg cagacctgta catttcacct tcgcatgtgg acggttcatc tgtgtcgttg
      421 atggaagctc tggcgtgtgg tttgccttgt ctcgtgtcgg atatccctgc caataaagaa
      481 tgggtattcg agaatcagaa cggctggctg ttccgcgatg gggatgtgga tcaccttgca
      541 gagaagattc tggcggcgat gaatcagcgt gagaaactgc ctgagatcgg caaagcaagc
      601 cgaagtgaag cagagaagcg cgcggattgg aaaaagaatg ccgcggcgct gatggaagtc
      661 tatcgaagtc taaaatgata cgtcattgcg agggcgatgt tctttcgccc gaagcaatct
      721 cataattgag tacaagattg cttcgtcaag ctgtcgccct cctcgcaatg acgggaaaag
      781 gaaaatatgt taagtaaaaa agaattattg gatggttttg gtcaactcaa cttgaaaaaa
      841 ggcgagacga tcattgtgca tacgtcgtat aaatcgttgg gcggggtgga aggcggcgcg
      901 gacacggtca ttgatgtgat gagtgaattg gtgggcaagg atggcacggt gctgttcccg
      961 gcgttcaact tccaatcgtg gacggagagt cattatttcg atgtgaaaga aacgccgtcg
     1021 aagatgggcg cgatcacgga gcaggcgcgc ttgcgcccga acgccttgcg gactccgcac
     1081 cccatctata gtttttctgc cttgggtgcc cgagcggaag agttttcaaa gaccgaagat
     1141 gtggaagcct acggtccgaa ttcggcgttt gcattgttcc ataagatcaa cggaaccatt
     1201 gtaagtattg gcttggattt caatagcact ttctcgatgc atcattacat cgaatataac
     1261 gttggctgta attatcgccg catcaaggag ttctctggca tttacgttgg ctatgaccgt
     1321 gtgccgaaac ttaagatgca ctcgatgttc gttcgtaacg atgacacagt gaagacttac
     1381 atcaaccctg gtatgaatga gttgttagag gcgggcgtga tcaaagaagt gcaggtcggc
     1441 gcagcgaaga tccattttgc gacggcaaat gatttctacg ataatatgtc ggtcattgtg
     1501 cgtgaacatc ctgagaaact gcacatcatc gaagagccaa aatatcgtaa gttttaattt
     1561 gtaggggcgg ggtttatgcc acggagtggt atttacccgc ccttttttta tgatataaaa
     1621 cagggcgagg agacctcgcc cctacacaat aaataatgaa accctacaac tccctcaaat
     1681 ccatcctcgc ggaattcttt cctctgcatc gaactctcgt ctctgatgac catgacaaga
     1741 cgttggagat tgtcggttcg tacatgcctg agtcttcgaa ctacacggtc gaaacctatg
     1801 cacctctcaa gcaagtttgg acgtggaaag tgccagaacg ctatgttgtc cacgaggcgt
     1861 atctggagat cgaaggcggc gagcggatcg tggacttcaa agataatccg ttgcacatcg
     1921 tttcgtattc actgcctgtt gataaagttt tgaatttcga cgaacttcaa ccgcatttgt
     1981 atttcaatga aaaacttccg cacaccgtgc cgtgggtctt caagtattac gaacgtagtt
     2041 ggggtttttg cttgccgaag aatactttcg ataaactccc gcgtgataaa aaatatcgcg
     2101 cgattatcaa ctgcgaattt atcgccgacc ccgcacaggg attcaa
//
LOCUS       DAOREF010000584         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40246_length_2143_cov_4.810824, whole genome shotgun sequence.
ACCESSION   DAOREF010000584 DAOREF010000000
VERSION     DAOREF010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40246_length_2143_cov_4.810824"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PKI33_11675"
     CDS             <1..1217
                     /locus_tag="PKI33_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNM37717.1"
                     /translation="SFSRRDIHSIAHEALRLLRNESHPSKQVLIQWKHENDKLTEERY
                     SSHLYSGTALKVEEIKPVYSDSSPTMFGFEVINAAFDAALTRDSKLIAFGEDVGKLGD
                     VNQGFKGMQEKYGEFRVTDTGIREATIMGQAIGMAMRGLRPIAEIQYLDYVLYALQTM
                     SDDLASLHWRTAGGQKAPVIVRTRGHRLEGVWHAGSPMSGILSLTRGMYVLVPRDMTR
                     AAGFYNTLLKSDEPSIVVEVLNGYRVKEKLPDNIGEITLPLGVPETIRQGKDITIVTY
                     GACCRIVLDAAEKLSKVGIDVEVIDVQSLMPFDIHGKIVESLKNTNRILFVDEDVPGG
                     ATAYMMQEVIEKQGGYFHLDSPARTLSAKAHRPSYGSDGDYWSKPNAETVFDVVYEMM
                     HEVNPTAFPLFS"
     gene            1242..>2143
                     /locus_tag="PKI33_11680"
     CDS             1242..>2143
                     /locus_tag="PKI33_11680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNM37718.1"
                     /translation="MATKVYVPRLGEGVDEVTVTKWLKQVGDSINELEPLLEVNTDKV
                     DTEIPAPATGTLLQIVAQEGVPAKVGELLAIIGKPGETVDSGQASIESAASSPTPVTQ
                     QTPAVIPQPQSINRDLGFISPVVAKIAAEHGVDLSQVNGTGLNGRITKNDVLNFVESG
                     KSKGTGQPSNISTFKPSNLTPDTGTLGTLIKHTTMRKSIAQHMVESKHTSPHVLTVME
                     ADMSRVAKHRAANKAAFERDGVNLTFTAYFMMAIVAGLKAYPQTNSSWTDEGLLIHKN
                     VNLGMAVSLGEDGLIVPVIKKAEDL"
BASE COUNT          564 a          505 c          537 g          537 t
ORIGIN      
        1 cgtcgttttc gaggcgcgat atccattcca ttgcgcatga ggcattgcgt cttctacgaa
       61 atgagtctca tccttcgaaa caagttctca ttcaatggaa gcacgaaaat gacaagctga
      121 ccgaagagcg ctatagttca catttgtact ctggcacagc tctcaaagtg gaagaaatca
      181 agccggttta ttcagacagc tcccccacca tgtttggttt tgaagtgatc aatgcagcct
      241 ttgatgcagc acttacccgt gactcaaaac taattgcttt cggtgaagat gttggcaagc
      301 ttggcgatgt caatcagggt tttaagggga tgcaagaaaa atacggcgaa ttccgtgtga
      361 ccgataccgg tattcgtgaa gctaccatta tgggacaagc cattggcatg gcaatgcgcg
      421 gattacgccc gattgctgaa attcaatacc ttgattatgt cttgtatgcg ttgcaaacca
      481 tgagtgatga ccttgcttca ttgcattggc gcaccgctgg cggacagaaa gcgcctgtta
      541 tcgtccgcac acgtgggcat cgtttggaag gtgtttggca tgcaggctcg cccatgtctg
      601 gcattcttag tttgactcgc ggcatgtatg ttttggttcc gcgcgatatg acccgcgccg
      661 ctggtttcta caacacctta ttgaaatcgg atgagccgtc tattgttgtt gaagttctaa
      721 atgggtatcg tgtcaaagag aaattgcccg ataacattgg tgaaattaca ttacccttgg
      781 gtgttcctga aaccattcgc caaggcaaag acattaccat cgtcacttat ggggcgtgct
      841 gtcgtattgt gttggatgct gctgagaaat tgagcaaggt aggaatcgat gtggaagtga
      901 tcgacgtcca atcgctgatg cccttcgata ttcatggcaa gatcgtggaa tcgttaaaga
      961 acaccaaccg tattctcttc gtagatgaag atgttcccgg tggggcaact gcctatatga
     1021 tgcaagaagt catcgaaaag caaggcggtt atttccattt agactcgccc gcacgtacat
     1081 tatctgccaa agcgcatcgt ccatcatatg gctcagacgg agattactgg agcaagccca
     1141 atgctgagac cgtgtttgat gtggtgtacg agatgatgca tgaagtgaat ccgactgcat
     1201 ttccactttt tagttgatag cacgaccaac tggagaaatt catggctaca aaagtttatg
     1261 taccccggct cggcgaaggc gtggacgaag ttactgttac aaaatggctc aaacaagttg
     1321 gcgattctat taatgaattg gaaccgctgc ttgaagtgaa cacagacaaa gttgacacag
     1381 aaatcccagc ccctgctacg ggaactctct tgcagatcgt tgcacaggaa ggtgtgcccg
     1441 caaaggtagg cgaactgtta gccatcattg gtaaaccagg ggaaactgtg gacagcggac
     1501 aagcgtccat agaaagtgct gcttcttcgc caacacctgt tactcaacaa actcctgctg
     1561 ttattccaca acctcaatca atcaaccgcg atcttggctt catctctcct gttgtggcaa
     1621 aaatcgccgc tgaacacggc gtggacttgt ctcaggtcaa cggcacgggg ctgaacggac
     1681 gcattacgaa aaacgatgtg ttgaatttcg tcgaaagtgg aaagtcaaaa gggacaggtc
     1741 aaccttccaa catttcaacc ttcaaacctt caaacctgac acctgacacc ggaacacttg
     1801 gcactcttat aaagcacacc accatgcgca aatcgattgc gcagcacatg gtcgagtcca
     1861 agcatacctc gccgcatgta ttgacggtga tggaagcgga tatgtctcgg gtggcaaagc
     1921 atcgtgccgc gaacaaagcc gctttcgagc gcgacggggt caacctcacc ttcacggcgt
     1981 atttcatgat ggcaattgtg gcagggctga aagcctatcc gcagaccaat tcttcgtgga
     2041 cggatgaagg cttgctcatt cataagaatg tcaacttggg tatggcggtc tcactgggtg
     2101 aagatggttt gatcgtgccg gtcatcaaga aagccgaaga cct
//
LOCUS       DAOREF010000585         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40373_length_2140_cov_2.706475, whole genome shotgun sequence.
ACCESSION   DAOREF010000585 DAOREF010000000
VERSION     DAOREF010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40373_length_2140_cov_2.706475"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..787)
                     /locus_tag="PKI33_11685"
     CDS             complement(83..787)
                     /locus_tag="PKI33_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HNM37719.1"
                     /translation="MSQNAAKRLYLMQVGIMPEYQIPIVCYLVQTGDGRNILIDTGLP
                     EILPEGETEFENGKDVIEQLKSLGLKPDDIDTVISTHYDGDHAGRHAAFTKAQYVVQR
                     AHHLDAATNPRFSEIRSQWDQPMDRIRLVDGDTELLPGLELIETSGHVPGHQSVLVRL
                     PKTGAILLTIDAVPFGEGFTRDVQDDGSNPDAEAIRTSTIKLLDLVNQEQIGLVIFGH
                     DTEQWKTLKKAPEFYE"
     gene            complement(830..1297)
                     /locus_tag="PKI33_11690"
     CDS             complement(830..1297)
                     /locus_tag="PKI33_11690"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNM37720.1"
                     /translation="MSTKTDTKKSASKSSNGFTADEKAAMKARAQELKAEAKASKNRE
                     EGEKTLLAAIAQMKDANDRSMGKRIHELVTAAAPELMPKTWYGMPAYADKDGKVICFF
                     QAASKFGVRYATFGFQPDAKLDDGNMWAASFALIKLTSAEEAKITALVKKAVR"
     gene            1402..1869
                     /locus_tag="PKI33_11695"
     CDS             1402..1869
                     /locus_tag="PKI33_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNM37721.1"
                     /translation="MPKSTKTDLKIRALMAVRDYGVNLTHFRNAMNEWAGLNATDMEC
                     LRLLSQKGTATPTELAKHTGLTSGATTAMLDRLEKAGLIERQPNPNDRRGTLIAPEKS
                     SSAKMAAWFESARNAQDALMSTYTESELEIIADVFERFAKLWDDERKKVQKKQ"
     gene            complement(1890..>2140)
                     /locus_tag="PKI33_11700"
     CDS             complement(1890..>2140)
                     /locus_tag="PKI33_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNM37722.1"
                     /translation="EKTGKTPKQFIAEAKKKKLTEFKDLMNWLKTDYGLGTGHARAVI
                     YVIQHGDTFTLTHTTGTHRDESGKLKLDGKKKTKKKSA"
BASE COUNT          521 a          571 c          448 g          600 t
ORIGIN      
        1 gattgtagaa cattttttct actcagtcaa ggctattctc gcaattttgc ttaccactgt
       61 gtgggtgcag attaaacact ttttactcgt aaaactctgg agcttttttg agcgttttcc
      121 actgctccgt gtcatgacca aaaatcacca acccgatctg ttcctgattg accagatcaa
      181 gcaatttaat cgtgctggta cggatcgctt cggcatccgg gttactcccg tcatcctgaa
      241 catcacgggt aaaaccttcc ccaaacggaa cggcatcgat cgtcaataaa atcgctcccg
      301 ttttgggcag ccgaaccagc acagattgat gtcctggcac atgcccactc gtttcgatca
      361 attccagccc tggcaacagt tctgtgtccc catctaccag tcgaatgcga tccattggtt
      421 gatcccattg gcttcgaatc tcagaaaaac gcggattggt tgctgcatcc aaatgatgag
      481 cacgttgaac aacatattgc gcctttgtga acgccgcgtg tcttccggca tggtcaccgt
      541 cataatgtgt cgaaatcacg gtatcaatat cgtccggctt caaacccagg ctcttcaact
      601 gctcaataac atccttcccg ttctcgaact ccgtttctcc ctcaggaagg atctctggaa
      661 ggcctgtgtc aatcaagata ttcctgccgt cacccgtttg caccagataa cacacaatcg
      721 gtatttgata ttccggcatt attccaacct gcatcaaata aagacgcttg gctgcattct
      781 gactcataac cttctcctca aatagtaggt gacacttact aaatctactt tatctcaccg
      841 cttttttcac gagtgcagtg atcttcgcct cttcagcgga tgtcaactta atgagcgcaa
      901 aactcgccgc ccacatattg ccatcgtcga gcttcgcatc aggctgaaag ccgaaggttg
      961 cataccgcac cccgaactta ctcgccgcct gaaagaagca aatgaccttg ccatccttat
     1021 ctgcataggc aggcatgcca taccaggttt tcggcatgag ttcgggcgca gcagcggtga
     1081 ccaattcatg gatccgcttc cccatcgaac gatcgttggc atccttcatt tgtgcgatcg
     1141 cagccaacag ggttttctcg ccttcttctc tattcttgct cgccttcgcc tctgctttca
     1201 attcctgggc gcgcgctttc atcgcggctt tttcgtcggc ggtaaatcca ttgctggatt
     1261 ttgaggcgct ttttttagta tcggtctttg tgctcataaa aatctcctta tctgacttgg
     1321 tcattttgac acttgcgtca aaatcaattt agttatataa taaagatact ttattataaa
     1381 gataatgtca agggatgttc catgccaaaa tcaacaaaga ccgatctgaa aatacgagca
     1441 ctaatggcag tgcgtgatta cggggtaaac ctcacccact tccgcaatgc catgaacgag
     1501 tgggcagggc tcaacgccac cgacatggaa tgcctgaggc tattatcaca aaagggcact
     1561 gccaccccca ctgagcttgc caaacatacg gggctaacct caggggcaac caccgccatg
     1621 ctcgaccgac tagaaaaagc tggcttgatc gaacgtcaac ccaatccgaa tgaccgccgc
     1681 ggcacactca ttgctccaga aaaatcgagc agtgcaaaga tggctgcttg gtttgagtcc
     1741 gcgcgaaatg cacaagatgc attgatgtcc acctacaccg agagcgagtt ggagatcatc
     1801 gccgatgtgt tcgagcgctt cgccaaacta tgggacgatg agcgtaaaaa ggttcagaaa
     1861 aagcaataga ccgagtccga tctatttttt caagcgctct tcttttttgt ttttttcttc
     1921 ccatctaatt tcagttttcc cgactcatcg cgatgtgtac ctgttgtgtg cgtgagtgta
     1981 aaggtatcac catgttgaat gacatagatg acggctctgg catgtcctgt accaagccca
     2041 taatcggtct ttagccagtt catcaggtct ttaaattcag tgagtttttt cttttttgcc
     2101 tcagcaataa attgtttcgg ggttttgcct gtcttttctt
//
LOCUS       DAOREF010000586         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40401_length_2139_cov_5.275432, whole genome shotgun sequence.
ACCESSION   DAOREF010000586 DAOREF010000000
VERSION     DAOREF010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40401_length_2139_cov_5.275432"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..859)
                     /locus_tag="PKI33_11705"
     CDS             complement(95..859)
                     /locus_tag="PKI33_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNM37723.1"
                     /translation="MNIITWNVNGIRAALGKNALDWAFEKKPDALCLQEVKARPEQLN
                     EEQTAQLKLPYIWNPAERPGYSGVATFYKTHPDETALGMDAPEFDVEGRIIQTRWGNV
                     RLFNIYFPNGQRGHDRVDYKLRFYARLLETCNTLHSKGELVVITGDFNTSHMPIDLKN
                     PKANEKTSGFMPEEREWVQKYLDNGFADAYRRLYPDRVQYTWWTYRLDARARGIGWRL
                     DYFLVSEALMPRVKDVIIHEQVMGSDHCPVELVLNV"
     gene            911..1297
                     /locus_tag="PKI33_11710"
     CDS             911..1297
                     /locus_tag="PKI33_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37724.1"
                     /translation="MDETPTKTILCVEDEPEMIDLIRLILSRKGYSVKGASGGVEGIR
                     LIKELHPDLVLLDLMMPDMDGWEVYQQMKAEASLREIPVIVVTAKAQNIDKVLGLHIA
                     KVDDYIAKPFSPQELVDSVDKVFSHK"
     gene            1350..1721
                     /locus_tag="PKI33_11715"
     CDS             1350..1721
                     /locus_tag="PKI33_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNM37725.1"
                     /translation="MPRPITIQNLSRTLEKPATVGYCDSFQCKLRGLMFRSHLDINDG
                     LLLVEKRNSRLDTSIHMFFVPFDLTVVWINSDLTVVDKVLAKSWKPAYFPKADAQYTL
                     EIHPDRWADYEIGDKVEFKNA"
     gene            1714..>2139
                     /locus_tag="PKI33_11720"
     CDS             1714..>2139
                     /locus_tag="PKI33_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37726.1"
                     /translation="MRKRFFIALLLLTLIVPFKPALAQDTGPIYIVQPGDSLYSIAAR
                     FSISLDELLAANGIADANSLAVGQQLIIPGLDGITGILDTKFINFGDSYRGLMRQTQV
                     QAPLFRKLNHVVSPSEFYVGSNMIVPVQDQTQYSKRISPT"
BASE COUNT          567 a          524 c          475 g          573 t
ORIGIN      
        1 accggaacct gcttgcaaaa ccaatcaatc ttttgtcacc ctagagagta atttcaactg
       61 aacttagcag aaattaatca gccatccaca aagtttacac atttaaaacc agctctaccg
      121 gacagtgatc ggaacccatc acctgctcat gaatgatgac atcttttacg cggggcatga
      181 gcgcctctga gacgaggaaa taatccaagc gccagccaat tccgcgggca cgagcatcga
      241 ggcgataggt ccaccaggtg tattggaccc ggtctgggta gaggcggcga taggcatctg
      301 caaagccgtt gtctaaatat ttttgaaccc actcgcgttc ctctggcata aagccagagg
      361 tcttctcgtt ggctttggga ttcttcaagt cgatgggcat gtgagaggtg ttgaagtcgc
      421 ctgtaatgac gaccagctct cccttagaat gaagcgtgtt acatgtttca agcaaacggg
      481 cataaaagcg taatttatag tccacgcggt catgtccgcg ttgtccattt gggaaataaa
      541 tattgaagag acgtacgttc ccccaacggg tttggatgat gcgcccttct acatcaaatt
      601 caggcgcgtc cattccaagt gctgtttcat ctggatgtgt cttataaaaa gtcgctaccc
      661 cactataccc tgggcgttcg gcaggattcc atatataagg cagcttcaat tgagcagtct
      721 gttcttcatt caattgttca gggcgagctt taacttcctg caaacacaac gcatctggct
      781 ttttttcgaa cgcccaatca agagcattct tgccaagtgc agcgcgaatt ccgttcacat
      841 tccaggtaat gatattcata cgaagttggt tggtctcttt atggtaaact tattttagaa
      901 ggcaaaaatt atggatgaaa cacctaccaa aaccatccta tgcgttgaag atgaacccga
      961 aatgatcgat ctgattcggt tgattctttc tcgaaagggg tatagcgtaa aaggcgcgtc
     1021 tggcggtgtg gaaggaattc gccttattaa agaacttcat ccagaccttg ttttgcttga
     1081 tcttatgatg cccgatatgg acggttggga agtataccaa caaatgaaag ccgaagcttc
     1141 tctgcgcgaa atccccgtta ttgttgtcac tgccaaagca caaaatatcg ataaagttct
     1201 tggtttacac atcgccaaag tggatgacta cattgccaag ccgtttagtc cacaagagtt
     1261 agtggatagc gtcgataaag tttttagcca taaataattt tcattccaaa acgggcggcg
     1321 aatacgccgc ccatttttca taaaacccca tgccacgccc catcaccata caaaatctta
     1381 gccgaacctt ggaaaagccc gccactgtgg gctattgtga ctcgtttcag tgcaaactgc
     1441 gcggattgat gttccgctcg catctggaca ttaacgatgg cttgctcctc gttgaaaaac
     1501 gaaactcgcg gctcgacact tccattcata tgttctttgt gccatttgac cttaccgtcg
     1561 tctggatcaa ctccgacctg acggtggtgg ataaggttct tgcaaaatca tggaagcccg
     1621 cctacttccc taaggcagat gcacaataca cgttggaaat acacccagac cgttgggcgg
     1681 attatgagat tggcgataaa gttgaattca aaaatgcgta aacgtttttt catagctctt
     1741 ctgctcctca ccctcattgt tcccttcaaa ccagcgcttg ctcaagatac cggacctatc
     1801 tacatcgtcc agccgggaga tagtctgtat tcgattgcgg cacgcttcag cattagcctc
     1861 gatgaactct tagctgcaaa tgggattgcc gatgccaact cgttggctgt tgggcaacaa
     1921 ctgatcatcc ccgggttaga tggcatcacc ggaattttag ataccaaatt cattaatttt
     1981 ggtgattcgt atcgcggctt aatgcgccag acgcaggtac aagctccgtt gttccgcaaa
     2041 ctcaaccatg tggtgagtcc cagtgaattt tatgtgggca gtaatatgat cgtgcctgtg
     2101 caagatcaaa cccaatacag caagcgcatc tcccctact
//
LOCUS       DAOREF010000587         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40571_length_2135_cov_3.541346, whole genome shotgun sequence.
ACCESSION   DAOREF010000587 DAOREF010000000
VERSION     DAOREF010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40571_length_2135_cov_3.541346"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKI33_11725"
     CDS             <1..659
                     /locus_tag="PKI33_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37727.1"
                     /translation="FIRMNLEHDGFQVGEAFNGKQAIQKIRDFTPDLILLDVMMPDID
                     GFEVLETIRDFSNVPVIMLTAKGEEDDRVRGLEHGADDYITKPFSPRELVSRIKAVLR
                     RTEGTGGSMHGLIEIDKRLKIDFERREIWLEGKLVKLRPTEYRLLYHLVQNAGWVVSH
                     DQLLQKVWGYEYRDEPHYVRLYINYLRQKLEKDPANPVYILTERGVGYRFVDFKRSKE
                     "
     gene            888..1331
                     /locus_tag="PKI33_11730"
     CDS             888..1331
                     /locus_tag="PKI33_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNM37728.1"
                     /translation="MSNLIRWEPAREMMTLREAMDRLFDDAFTRPLSLSGNSWSIPAV
                     DMYQTDNEVVVKAALPGIKADEVQLNVTGEVLTIKGETKQENETKEKAYHIREQRWGT
                     FERSLVLPTEVVVDKAKADFENGILTITLPKAEEVKPKTINIKTK"
     gene            1413..>2135
                     /locus_tag="PKI33_11735"
     CDS             1413..>2135
                     /locus_tag="PKI33_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNM37729.1"
                     /translation="MPQDFSSLRINPDRMLDAFTQLAAIGSTGDGGVNRTTFSEAHLH
                     ARKWFREHIQQSGLEFRTDGAGNHSAVLPCDDPNARTLILGSHLDSVPKGGRFDGALG
                     VMTALEVLKTVRENGIKLKVQLEAMDFTDEEGTHLSLFGSSAISGHLSREALKNPYSG
                     HENFTAGLARAGLTEESLLTAKRPKESLAGYLELHIEQGKRLEKRGLNIGIVSAIVGY
                     GSYRMAYIGRADHAGSAAMEDRL"
BASE COUNT          540 a          494 c          584 g          517 t
ORIGIN      
        1 gctttattcg tatgaacctg gagcatgatg gttttcaggt aggggaggcg tttaacggca
       61 agcaagcaat ccaaaagatt cgtgatttca ctcccgacct tattttgctt gatgtgatga
      121 tgccggatat tgacggcttc gaagtgcttg agaccatccg cgattttagt aatgtgccgg
      181 tcattatgct caccgccaaa ggtgaagagg atgaccgtgt gcgtggactc gaacatggtg
      241 cggacgatta cattaccaag ccgttcagcc cgcgtgaatt ggtcagccgt attaaagccg
      301 tactgcgccg caccgaaggc acaggcggct ccatgcatgg actgatcgag atcgataaac
      361 gcttgaagat cgattttgaa cgccgtgaga tttggctcga aggcaagctc gtcaagcttc
      421 gccccacaga atatcgattg ctgtatcatc ttgtgcagaa tgcaggctgg gtggtttctc
      481 acgaccaatt gctccagaaa gtttggggct atgaataccg cgatgaaccg cactacgtcc
      541 gtttgtatat taactatttg cgccaaaagt tggagaagga tccggctaat ccggtttaca
      601 ttctcactga gcgtggagtt ggctatcgct ttgtcgattt caaacgcagt aaggagtaac
      661 ccatgacgga gcaaaaacct gagaacaagt gggtgacggc ggcaaagctg gttggcgttt
      721 gtgtgacctg cattggtgtg atttacggat tggttgtgct tttcaccacc ttcaacccct
      781 gaactgcttc tcttataaaa cgaaaacaaa acgcttgcgt ttttctaacg caggcgtttt
      841 aagttaatgg acgtaagaca aaattcaaat cctgcaagga ggtagctatg tcaaacttaa
      901 ttcgttggga acccgctcgc gagatgatga ccctgcgtga agccatggat cgcctcttcg
      961 acgatgcgtt tacccgcccg ctcagccttt ccggaaattc gtggtctata ccggctgtgg
     1021 atatgtacca aacagataac gaagttgtgg tgaaggctgc tttgcccggc atcaaagccg
     1081 atgaagttca gctcaatgtg accggtgagg tgcttactat taaaggtgag accaagcagg
     1141 agaacgaaac caaagagaag gcatatcaca ttcgcgaaca gcgctggggt acgttcgaac
     1201 gctcgctcgt tctgcccacc gaagttgtag tggataaagc caaagcggac tttgagaacg
     1261 gcattctgac catcacgctc cccaaagcgg aagaggtcaa gcccaaaacg atcaatatca
     1321 agaccaagta atatttcgcg tacgaaacag gagccgggtt ctaacggctc ctgtttttgt
     1381 tatgataggc agaatatagg agattttccc aaatgcctca agatttttct tcccttcgca
     1441 tcaacccaga ccggatgctg gatgctttca ctcaattagc cgccatcggc tcgaccggtg
     1501 atggtggcgt aaatcgcact acgttcagcg aggcgcatct tcacgcccgc aaatggtttc
     1561 gcgaacacat ccagcaaagc ggattggaat ttcgcaccga tggggcaggg aatcactccg
     1621 cagttttgcc ttgcgatgat ccaaatgcgc ggacgctcat tctcggctcg cacttggatt
     1681 ctgttcccaa gggcgggcgc ttcgacggag ccttgggcgt gatgacggca cttgaggtgt
     1741 tgaaaacggt tcgagaaaac ggaatcaagc tcaaggtcca gttagaagcg atggacttta
     1801 cagacgaaga aggcacacac ttgagtctgt ttgggagcag tgccatttcc ggtcatttga
     1861 gtcgagaggc gttgaaaaat ccatacagcg gacatgagaa tttcacagca gggttagcac
     1921 gcgccgggct gaccgaggaa agtttgttga ctgccaagcg accgaaagaa tcgttggcag
     1981 gatatttgga gttgcatatt gagcaaggga aacgcttgga gaagcgcgga ctgaatattg
     2041 gcattgtttc agcgattgtg gggtatggct cgtatcgcat ggcgtacatt gggcgtgccg
     2101 atcatgccgg gtctgccgcg atggaagacc gtttg
//
LOCUS       DAOREF010000588         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40724_length_2131_cov_2.919075, whole genome shotgun sequence.
ACCESSION   DAOREF010000588 DAOREF010000000
VERSION     DAOREF010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40724_length_2131_cov_2.919075"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="rplL"
                     /locus_tag="PKI33_11740"
     CDS             <1..371
                     /gene="rplL"
                     /locus_tag="PKI33_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNM37730.1"
                     /translation="AKLVDELSTLSVLEAADLVKMLEEKWGVSAAAPVAVAAVAGGAA
                     ASAEPVEEKTEFDVVIKDAGAKKIDVIKVIRQLTSLGLGEAKTLAETAGGKVLSAVGK
                     DAAMDAKKKLEEAGAVITVE"
     gene            complement(431..1108)
                     /gene="lexA"
                     /locus_tag="PKI33_11745"
     CDS             complement(431..1108)
                     /gene="lexA"
                     /locus_tag="PKI33_11745"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNM37731.1"
                     /translation="MMMVRKSKGLGERHQKILDFIASYQRDHKHPPSIREIGEHCDIS
                     STSVVNYYLDQLEKSGNIERDRKISRGVRLTGSTPLGDMFRVPVLGVIQAGEPIPVPS
                     NADFNAFTPEDSVEIAMSLMPTKEKGKELFALKVQGESMIDAMVNDGDIVILKPTQDA
                     RNGDMVAVWLSDRNETTLKFFFKEKDKYRLQPANPTMSPIYVNKNEALEIKGKVVMVI
                     RKVERYM"
     gene            complement(1221..>2131)
                     /gene="corA"
                     /locus_tag="PKI33_11750"
     CDS             complement(1221..>2131)
                     /gene="corA"
                     /locus_tag="PKI33_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNM37732.1"
                     /translation="RDRRGLLWVDFEAEPEESALPILNGFGFHQLAIDDALQQTHAPK
                     IDDWGDYLYIVLNYMHLTKATEPWDTEIDELDIFLGRNYVITHHDNPITSINETWNTS
                     QRDPRYSQDGADHLLYKIIDAIVMNYMPIIEKIDEEIDWVEDQVFDRPSSQTLARLFT
                     LKRVLLAMRRILLPQREVLNKMARDDFQVIDRKDRIFFRDIYDHLVRLHDVNESLRDL
                     VGGALDTYLSVINNRMNEVMKTLTIITTLFMPLTFVTGFFGMNFFAANPPYESWTLPA
                     VFYGTLSLMLLSPIIMFIWMRRHTWV"
BASE COUNT          508 a          488 c          578 g          557 t
ORIGIN      
        1 tcgcaaaact cgtggatgaa ctttccacac tttccgtcct tgaggcggct gatctcgtaa
       61 aaatgctcga agagaagtgg ggcgtttccg ccgccgctcc tgtggctgta gctgctgtcg
      121 ctggcggcgc tgctgcttcc gctgaacctg ttgaagagaa gactgagttc gatgtggtca
      181 tcaaggacgc tggcgccaag aagatcgacg tcatcaaggt cattcgtcag ttgaccagcc
      241 tcggcctcgg cgaagccaag accctcgctg aaaccgctgg cggcaaggta ctctccgctg
      301 ttggcaaaga tgccgcgatg gacgccaaga agaagctcga agaagctggc gccgttatca
      361 cggttgagta agaagcaagt cttaaataaa aaaggacggc tcgcaagagc cgtccttttt
      421 tatttgttta ttacatgtac cgttctactt tgcgaatgac catgaccacc ttgcctttga
      481 tctccagagc ctcgttcttg ttgacataga tcgggctcat ggtggggttg gcgggttgca
      541 ggcggtactt gtctttttcc ttgaagaaga atttcagagt ggtttcgttg cggtctgaaa
      601 gccataccgc aaccatgtcg ccgttgcggg cgtcctgggt gggtttgagg atgacgatat
      661 caccatcgtt taccatggcg tcgatcatcg attctccctg gacttttagc gcgaacaatt
      721 ccttgccttt ttctttcgtt ggcatcaaag acatggcaat ttcaaccgag tcttcggggg
      781 taaaggcatt gaaatcagca ttggaaggga cagggatggg ttcaccagct tgaatgacac
      841 caagcactgg aacgcggaac atatcaccaa gcggtgtgga gcctgtcaga cgaacaccgc
      901 gggatatttt gcgatcacgt tcgatgttgc ccgatttttc cagctgatcc agataataat
      961 taactacaga agtagaggaa atgtcacaat gctccccaat ctcccgaatt gaaggggggt
     1021 gcttgtgatc gcgttgatag ctcgcaatga aatcaaggat tttctggtgg cgttcgccca
     1081 gacctttgct ttttctaacc atcatcatat tagcctccac gagcgcattt ttcgctcatt
     1141 tgtgctagga atgataaccg aacgcctgtt ctgtgtcaag gattttttta gcagatttat
     1201 gcggcaaacc atggggccgc ctatacccag gtatggcgac gcatccagat gaacataatg
     1261 attggggata acagcatcag gctcagggtc ccgtaaaaga ccgctggcag ggtccagctt
     1321 tcgtagggag gattcgcggc gaagaaattc atcccaaaaa aacctgttac aaaagtcaac
     1381 ggcatgaaaa gggtggtgat gatggtaagg gttttcatga cctcattcat gcggttgttg
     1441 atgacggata ggtaggtatc taatgcgccg ccgacgagat cgcgcagact ttcatttacg
     1501 tcatgcaaac ggacgaggtg gtcgtaaata tcgcggaaaa agatacggtc tttacggtca
     1561 atgacctgaa agtcgtcacg tgccattttg ttgagtactt cacgctgggg cagcagaatg
     1621 cgccgcatgg caagcaggac acgtttgagt gtgaataatc tggcaagggt ttgtgaactg
     1681 gggcggtcaa agacctggtc ttcgacccaa tctatctctt catctatttt ttcaattatg
     1741 ggcatgtaat tcatcacaat ggcgtcaatg atcttgtaga gcaggtggtc ggcgccatct
     1801 tgcgaatagc gcgggtcacg ctgactggta ttccaggttt cattaatgga agtgatgggg
     1861 ttatcgtggt gggtgatgac atagtttcgc cccaggaaga tatccaactc atcgatctcc
     1921 gtatcccagg gttcggtggc ttttgtcaga tgcatatagt ttagaacgat atagaggtag
     1981 tctccccaat catcgatctt gggtgcgtgg gtttgttgca gggcatcgtc aatggctagt
     2041 tggtggaatc caaaaccatt aaggatgggt aatgcacttt cttcgggttc ggcctcaaaa
     2101 tccacccaaa gcagtccgcg gcggtctcgt a
//
LOCUS       DAOREF010000589         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40748_length_2130_cov_4.451566, whole genome shotgun sequence.
ACCESSION   DAOREF010000589 DAOREF010000000
VERSION     DAOREF010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40748_length_2130_cov_4.451566"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..646)
                     /locus_tag="PKI33_11755"
     CDS             complement(2..646)
                     /locus_tag="PKI33_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNM37733.1"
                     /translation="MKLISINAGKKHTLVNKGKTEITGIYKNPLAGAVHITKLGIADD
                     YIGSPKHHGGPDQALYVYGEADYQWWEKEIGTKLGPGMFGENLTISELACSGFNIGDY
                     LYIGNVTLQVTAPRIPCGTFASKMEDPQWVKKFSAAERPGFYVRVLQEGELSASEEVR
                     VEKYTGATLSLVQVYRDHYDKEGRNEAVLRRHIDSPLSIRMREKYERELEALLH"
     gene            complement(730..1584)
                     /locus_tag="PKI33_11760"
     CDS             complement(730..1584)
                     /locus_tag="PKI33_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37734.1"
                     /translation="MQTSTSWRSFRMAAWLGWVIESNWTDPFLFAVYSIVKPISGAAI
                     LVIMYGIISQGNFDSALFPYIYLGNAFYIYVGAVMTGVSWAVVDDREHYKTLKYMYIA
                     PIHIPLYLLGRGVARFVTGSIAVFITILAGVLFLNVKINIMQVDWLLFFTTLIVGVVM
                     LALLGLLLAGLTLTLARHEGFIGEATAGALYIFSGAIFPLDVLPTWLRPIGYFMPTTY
                     WLELLRRSLVGNVAQAFPTLANFSNLQILGVMVGLSIFFGVISVYVFRWCDGIARERG
                     LIDRTTNY"
     gene            complement(1633..>2130)
                     /locus_tag="PKI33_11765"
     CDS             complement(1633..>2130)
                     /locus_tag="PKI33_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37735.1"
                     /translation="MALGGMLATAMRAVAVTLLGIWLFDVAFSVSSYVQLFLVFMLAM
                     IALYGMGMMMASAFLMFGREAWHMANLAQEPIFLASGFYFPIKSLPFWVAAGASVIPL
                     TLGMDAMRQLIFPTGFKFGFLTVGTEIAILAVLSVVFVVTAKVLLAYVEKLAVREGRI
                     TESRR"
BASE COUNT          633 a          586 c          440 g          471 t
ORIGIN      
        1 cttaatgaag caaagcttct aattcacgtt catatttttc ccgcatccga atggacagag
       61 gtgaatcaat atgccttcgc aggacagcct cgtttcgccc ttctttatca taatggtcgc
      121 ggtatacctg caccaaagaa agggttgccc ctgtatattt ctccacacgg acttcttcac
      181 tcgctgaaag ctccccttct tgcaatacgc ggacgtaaaa cccggggcgc tcggctgcgc
      241 tgaatttctt cacccattgc gggtcttcca ttttcgaagc gaacgtccca cagggaatgc
      301 gaggagccgt cacttgtaac gtcacgttgc caatataaag ataatctcca atattaaaac
      361 cgctacatgc caattcacta atagtgagat tctctccaaa catgccggga cccaactttg
      421 ttccaatctc tttttcccac cattgataat ccgcttcgcc ataaacataa agcgcttgat
      481 cgggtccgcc gtgatgtttt gggctgccaa tataatcatc ggcaattcca agcttggtaa
      541 tgtgcacagc tccagcgagc ggatttttgt agatgcctgt aatctccgtc ttacctttat
      601 tgactagagt gtgtttcttt cctgcattga tgctgattag tttcattcta tatcctttaa
      661 atacaaacgt gctgacatga gcagatttta tcccatccat gccagcacga accctacttt
      721 ggaattattt tagtaatttg ttgtgcggtc gatcagccct ctttcgcggg caatcccgtc
      781 acaccagcgg aagacataca cactgatcac cccaaagaaa atgctcaacc cgaccatgac
      841 gccaagaatt tgcaagttac tgaagtttgc caaggttggg aaggcttgcg ccacattccc
      901 taccagcgaa cgacgcaaca attcaagcca ataggtagtt ggcataaaat acccgatcgg
      961 acgtaaccaa gtaggcaata catccaacgg gaagatggcg ccgctgaaga tatacaacgc
     1021 gccagcggtg gcttcgccga taaagccttc gtggcgagcc agtgtgaggg ttagacccgc
     1081 caaaagtaag cccagaagcg ccagcatcac cacaccaacg atcaaagtcg taaagaacaa
     1141 caaccagtcc acttgcataa tgttgatctt tacgttcaag aacaaaaccc cagccaaaat
     1201 ggtgatgaac acagcaatgg agccggtcac aaaacgcgcc acaccgcgac caagcaaata
     1261 cagtggaata tgaatcggtg cgatgtacat gtacttgaga gtcttgtagt gttcacggtc
     1321 atcgaccaca gcccaagaga caccggtcat taccgcaccg acataaatat agaaggcatt
     1381 acccaagtaa atgtatggga acaaggcact atcaaaatta ccttgtgaaa tgatgccata
     1441 cataatgacc aaaatcgccg cacctgaaat cggcttgacg atggaataca ccgcgaacag
     1501 aaagggatct gtccaattcg actcgatcac ccagccaagc cacgccgcca tacgaaacga
     1561 acgccatgaa gttgaagttt gcataaattc tccgtaacag gaatcaagaa atcttttcag
     1621 aatcccttgt ccctatcgtc tgctttcagt aattcttccc tcgcggacag ccagtttttc
     1681 aacatatgcc agcaaaacct ttgccgtcac aacaaagacc acagaaagaa ccgccaaaat
     1741 cgcgatctct gtgcctacag ttaaaaagcc aaacttgaac ccagtcggga agattaactg
     1801 acgcatcgcg tccatgccca aggtgagcgg aatgaccgaa gcccccgccg ccacccaaaa
     1861 cggcagcgac ttgatcggga agtaaaagcc cgaagcgaga aagatcggtt cttgtgccaa
     1921 attcgccata tgccaggctt cacgcccaaa catcagaaat gccgaagcca tcatcatgcc
     1981 catgccatac aaagcgatca tcgccagcat gaatacaaga aatagctgca cataactcga
     2041 tacagaaaat gccacatcaa agagccagat gccgagcaag gtcacagcca cggcacgcat
     2101 ggcagttgcc aacataccgc ccagcgccat
//
LOCUS       DAOREF010000590         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40825_length_2128_cov_3.843222, whole genome shotgun sequence.
ACCESSION   DAOREF010000590 DAOREF010000000
VERSION     DAOREF010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40825_length_2128_cov_3.843222"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PKI33_11770"
     CDS             complement(<1..1439)
                     /locus_tag="PKI33_11770"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNM37736.1"
                     /translation="MTEIFISYCRRNKGFTEQFIQGLAEQNYPADKIWIDWNNIPPSS
                     KWEDEIRKGIEACNAVVFILSPDWILSNECRKELEIAIEYNKRLFPIIHQNIDPQSSP
                     AALASLNWIFFREADDFKGAMQKLMAAINTDLDWVKQHTVLLKRANEWNSKKQDGSYL
                     LRGSELQGAELWLSKSSDNKQPHPTPLQSEYIFASRQDASRRQRNTLIGVSAALVVSI
                     LLAIAAIVGGVEAVRKSQQALASQLAAQSITLVDTQPDLSLLLSLESNYIGDTLGDAD
                     PASLGSLVSSINSVPQLDTYLRAHAGEIREVTFSSNGRWVAATGNAINDQGVVTVWDL
                     ASKENPIADQQFIGGTQRILGLAFSPDSKRIAAAGDEKKIFIWDPQTCCETVKEFEID
                     NKARSLKFVTLNGKEYIAIASGQQITFWDASTGKKNDAMTLQISTTDETVRILSLAVA
                     PTNDMVAAGSEDGNITVWELKTKEVKFQA"
     gene            1642..>2128
                     /locus_tag="PKI33_11775"
     CDS             1642..>2128
                     /locus_tag="PKI33_11775"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNM37737.1"
                     /translation="MKLNLNNTAFVFPGQGSQTVGMGKSLAEEQTIAKEVFAEADSIL
                     GFALSDLMWNGPDVELNDTVNTQPALFIHSIASFRTFTRLYPDLVPAALAGHSLGELS
                     ALTASGALSFEDGLRLVRKRGELMKRAGELAPGSMAAILGVDIPTLDKVCAEASVTDE
                     LV"
BASE COUNT          524 a          501 c          487 g          616 t
ORIGIN      
        1 caagcttgga atttaacctc ttttgtcttt agttcccaaa ctgtgatatt cccatcttca
       61 ctgcctgctg cgaccatgtc gtttgttggc gcaacggcaa ggctaagaat acgcacagtt
      121 tcgtcggtgg tggagatttg taacgtcatg gcatcgttct ttttgcctgt cgatgcgtcc
      181 cagaatgtga tttgttgacc ggaggcaatg gcgatatatt cttttccatt caaggtaaca
      241 aatttcaaag aacgtgcttt gttgtcgatc tcaaattctt ttacggtttc acagcaagtt
      301 tgcggatccc agataaagat ttttttctca tctccagccg ctgcaatgcg tttgctgtct
      361 gggctgaaag caagacctaa aatacgttgt gtgccgccaa taaattgttg atctgcgatc
      421 gggttttctt ttgaagccag atcccaaact gtgacaacgc cttggtcgtt gatcgcattc
      481 ccggttgccg caacccatct tccattcgaa ctgaatgtca cttcccgaat ctcacctgca
      541 tgtgcccgca aataagtatc caactgtggg acgctgttga tgctggaaac caaacttcct
      601 aaacttgccg ggtctgcatc gccaagagtg tcgccaatgt aatttgactc aaggctcaga
      661 agtaaagaaa ggtcaggttg tgtgtctacc aaagtgatgg attgagcggc aagctgactc
      721 gccagtgctt gctgactttt acgcaccgcc tccactccgc cgacgatggc ggcaattgcc
      781 aataaaatag aaacgaccaa tgctgcgctg acgccaatca aggtattgcg ttggcggcgg
      841 cttgcatctt ggcggctggc aaagatgtat tcactttgaa gtggcgttgg atgtggttgt
      901 ttattgtcag aacttttaga aagccataac tctgcccctt gtaattcact accgcgtaac
      961 agataactgc catcttgttt ttttgaattc cattcgttgg ctcgcttcaa aagaacagta
     1021 tgttgtttga cccaatccag atcagtattg atcgctgcca ttaatttttg catggcaccc
     1081 ttgaagtcat cggcttcgcg gaagaaaatc caatttaagg atgccaatgc ggcaggagaa
     1141 ctttgcgggt caatattttg gtgaatgatg gggaagagcc gcttgttgta ttcaatcgca
     1201 atctctaact ctttgcggca ctcgttggaa agaatccaat cagggctaag aatgaagacc
     1261 acagcattac atgcttcaat gccttttcga atctcatcct cccatttaga ggaaggtgga
     1321 atattgttcc aatcgatcca gattttgtct gcggggtaat tctgttctgc tagcccctgt
     1381 ataaattgct cggtaaagcc tttgttgcgg cgacaatatg agatgaaaat ttctgtcatg
     1441 atctggctcc ttgattcgca tgatctgaaa agcacttttt gcccatcaaa cctgcttttg
     1501 gcggcataga aaacttgcaa tgattttgca attttactcc tgcccatgcg tgaccatatt
     1561 tgcaattttg tgtttaatag ccatgcctaa caatgctgta aatagctgaa atatattgat
     1621 ttttagccac aaggagacgg aatgaaatta aatttgaaca acacagcctt tgtcttcccc
     1681 ggacaaggct ctcaaacggt agggatggga aaatcccttg ccgaagaaca aacaatagcc
     1741 aaagaagtct ttgccgaagc ggattcgatc ctcggctttg cgctctcaga cctgatgtgg
     1801 aatggaccag acgttgaatt aaatgatacg gtcaacacgc aacctgcatt atttatccat
     1861 tccattgctt cctttcggac attcacccgc ctctacccgg acctcgtgcc tgctgcactt
     1921 gccgggcatt cacttggcga gttatcggct ctgactgcat ctggagcctt atcttttgaa
     1981 gacgggttac gcctcgtgcg caaacgcggt gagttgatga agcgtgcagg tgaactggct
     2041 cccggtagca tggctgccat cctcggcgta gacattccca cacttgataa agtttgtgcc
     2101 gaagccagtg tcacagatga gttggtac
//
LOCUS       DAOREF010000591         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_40956_length_2125_cov_4.685990, whole genome shotgun sequence.
ACCESSION   DAOREF010000591 DAOREF010000000
VERSION     DAOREF010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40956_length_2125_cov_4.685990"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKI33_11780"
     CDS             <1..417
                     /locus_tag="PKI33_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37738.1"
                     /translation="DICFSGDVGGVRIPGFQYLRAPMPPPELHFGRWRESLARLRGMK
                     FAHIAPTHFGMFDDVQWHLSTLDETLAAMEKWLETVMAQDPSIEKLREEFTLWMEEEG
                     KAKNLSEDVLRAYSLSNPVAMSADGLMRYWKKVRSA"
     gene            495..1421
                     /gene="argF"
                     /locus_tag="PKI33_11785"
     CDS             495..1421
                     /gene="argF"
                     /locus_tag="PKI33_11785"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNM37739.1"
                     /translation="MKDFIAISDYSPEEIEDLLDLAVKLKKQYFKKGNKPIFQGKVLG
                     MIFQKPSLRTRVSFDMAMRHCGGDALYLSPNEIGLGKRESIADIARVLSGYVQALMAR
                     TFDHAHVVELAKWADIPVINGLSDYNHPCQGMADALTIIEKFGKDSKGLNVSYVGDGN
                     NVTVSLMHVAAQLGWNMTVGTPEGYDVNPQAVEIAQNIARKTGSKLTFVRDVHEAVKN
                     AHVIYTDTWTSMGQEEETAKREQIFPPYQVNAKLVSEADKDVIVMHCLPAHRNHELTD
                     EVADGPHSVIFQQAHNRLHAQKAILARLFDVA"
     gene            1455..>2125
                     /locus_tag="PKI33_11790"
     CDS             1455..>2125
                     /locus_tag="PKI33_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM37740.1"
                     /translation="MNTQDFIKIEEQYGAHNYHPLDVVIEKAEGVWVYDVEGRKYLDC
                     LSAYSAVNQGHVHPEILNALLEQAKKVTLTSRAFRNDQLPLLYKELSEMTGYDMSLPM
                     NSGAEAVETAVKLARKWAYQVKKVPRHQAEIIVAGNNFHGRTVTIVSFSTEPLYRDDF
                     GPFTPGFVVVEYGNLDALEKAITPNTAAVLLEPIQGEAGVIMPPAGYLKKVAELCKKN
                     NVLYIS"
BASE COUNT          566 a          449 c          569 g          541 t
ORIGIN      
        1 gatatatgtt ttagtgggga tgtgggcgga gtgcgtattc cgggctttca atatttgcgt
       61 gcgcctatgc caccacccga gttgcatttt gggcgttggc gtgaaagttt ggcgcgtttg
      121 cgcggaatga aatttgcgca tatcgccccg acccattttg gcatgtttga tgatgtgcaa
      181 tggcatttga gcaccctcga tgagacgctg gctgcaatgg agaaatggct tgaaacagtg
      241 atggcgcaag acccgtccat tgagaagttg cgcgaagaat ttacgttgtg gatggaagaa
      301 gaaggtaagg caaagaactt gagcgaagac gtgttgcggg cttattctct ctctaacccg
      361 gtggcgatga gtgccgatgg gctaatgcgg tattggaaga aagttcgcag tgcgtaattt
      421 gtcatattct ttgcgtgatg cttgaaacat ggcaaaatag actaaaaaag atatactttc
      481 cctgaggagc acacatgaag gactttattg cgatttctga ctattcacca gaagaaattg
      541 aagacctgct cgaccttgct gtaaaactca aaaaacaata tttcaaaaaa ggcaacaaac
      601 cgatctttca aggcaaagtg cttggaatga tcttccaaaa gccgagcctg cgcacgcgcg
      661 tttcttttga tatggcgatg cgacattgtg gcggcgatgc cttgtatctt tctccaaacg
      721 aaattggttt gggcaagcgc gaatcaattg cagatattgc gcgagtgtta tctggttatg
      781 ttcaagcatt gatggcacgt acctttgacc atgctcacgt ggtggaactc gccaaatggg
      841 cagatattcc ggtgatcaac ggcttgagcg attacaacca tccctgtcag ggcatggcag
      901 atgcattgac catcattgaa aaatttggca aagactcaaa aggtttgaat gttagttatg
      961 tcggtgatgg caataacgtc acggtttcgt tgatgcatgt agcagctcaa ctgggttgga
     1021 acatgacggt tggcacgccc gaaggctacg acgtcaaccc gcaggcggtg gaaattgctc
     1081 agaacatcgc cagaaaaacg ggcagcaagt tgacttttgt ccgcgatgtg catgaagcag
     1141 tgaagaatgc tcatgtgatt tataccgata cgtggaccag catggggcag gaagaagaaa
     1201 ccgccaagcg cgaacagatc ttcccgccgt atcaggtcaa tgccaagttg gtgagcgaag
     1261 cagataaaga tgtgatcgta atgcattgtt tgcctgcaca ccgcaaccac gagttgactg
     1321 acgaagtagc ggatggtcca cactcggtga tcttccagca ggcgcataat cgcttgcacg
     1381 cgcagaaggc aattttggcg cgtttattcg atgtcgcata aagatattca tagtttttag
     1441 aggataccca ttttatgaat acccaagact ttatcaagat cgaagaacaa tatggcgcgc
     1501 ataattacca tccattggat gtggtcatcg aaaaagcaga aggggtgtgg gtttacgatg
     1561 tggaaggcag aaaatacctt gattgtcttt ctgcatattc tgctgtaaac caagggcatg
     1621 ttcatcctga aattttgaat gcattgttgg aacaggcaaa gaaggtcaca ctcacttcgc
     1681 gtgctttccg caatgaccag ttgccgcttc tttataaaga actttctgaa atgaccggct
     1741 acgatatgtc tctgccgatg aactctggtg cagaagcagt tgagacggcg gtgaagcttg
     1801 ctcgcaagtg ggcatatcag gtcaagaagg tgccgcgcca tcaggcggaa attatcgtag
     1861 cgggcaacaa cttccacggg cgcaccgtca ccattgtttc attctccaca gaaccgctct
     1921 atcgggatga tttcggtccg ttcacgccgg gttttgtggt ggttgaatat ggaaaccttg
     1981 atgcgcttga aaaggcaatt acgcccaaca cggctgcggt tttgctcgaa ccgattcagg
     2041 gagaggcagg ggtgatcatg ccgccggctg gatacttgaa gaaagttgcc gagctttgca
     2101 agaagaataa tgtactttat atctc
//
LOCUS       DAOREF010000592         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41141_length_2120_cov_3.446973, whole genome shotgun sequence.
ACCESSION   DAOREF010000592 DAOREF010000000
VERSION     DAOREF010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41141_length_2120_cov_3.446973"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..718)
                     /locus_tag="PKI33_11795"
     CDS             complement(158..718)
                     /locus_tag="PKI33_11795"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HNM37741.1"
                     /translation="MSLFIILLSLALWGVVHSFLASHLAKDLFGMKRLYRLLYNIFSL
                     VSFAPILYLVATLEDAALYQIPSPWSLVMRGGQLLAALLLLVAVLQTDTLSFVGLRQL
                     FEEEKTGQLVTRGLYKVVRHPLYLFSLLFLWLSPSMSINSLVFNIGVTAYFIIGAYFE
                     ERKLLRDFGEAYADYKRRTSFLIPGL"
     gene            complement(822..>2120)
                     /locus_tag="PKI33_11800"
     CDS             complement(822..>2120)
                     /locus_tag="PKI33_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37742.1"
                     /translation="VFVMNNVHEKNPVLFQTRWAMNFLAGPMTRTQIRDLNALVGAGE
                     VAAPQPKPKPVSAPKVTATPPPVDHVQPISVPSAPAQPKKTPTRSTSSSVLESSNGGN
                     LTKPPVPAGIREYYLPQNYSMPEAFSAAGKPQAYGANIQGVVYKPVLLASAQIRVLDR
                     KYGVDVEVTKTALVDSLDRRGIVRWEDFTYAGPSLDKLESGPAPSSKYGSIEAPLNDS
                     KLMTALQKDFTDWVFRNSSVEARANVALKVFAGPDVSQADFMKACADTAREARDTEIA
                     KKTSAIEKKIKTLEDKLGREERELREDEAELQNRNIESGANLLELGASVLGLTRKKSV
                     TTQFTKHRLAQSAKAEVQESQETISKLTKDLNDLIREREQVIREISDKWGRIVSESNE
                     VTINPKKTDIYVNIFGIAWKPYYLIQAGGETTELAAFGVD"
BASE COUNT          565 a          490 c          533 g          532 t
ORIGIN      
        1 ataaaaattt atgcccgtcc acctaaacgc tttcaaaggc gggtcgggca cgcgggggca
       61 agattatcat gcctgaaatg gggtgtcaag attttatgaa aaatggtccg ctagacagta
      121 gttgacggat gtccaactaa tgtcaatggt ctgttcttta caagccaggg attaaaaatg
      181 aggtgcggcg tttatagtct gcataggctt cgccaaaatc acgcaggagt ttacgctctt
      241 caaaataagc accgataatg aaatatgccg tcacaccaat gttgaacacc aacgaattga
      301 tactcatcga cgggcttagc cataaaaaca gcaagctaaa taagtaaagc ggatgccgta
      361 caactttgta caaaccgcgc gtcacaagtt gaccggtttt ctcttcttca aacaattgac
      421 gcaaaccgac aaaggaaagt gtatctgttt gtaatacagc cactagaagt aacaacgccg
      481 caagtaactg tccgccacgc atgaccaaac tccacgggga tgggatttgg tagagagccg
      541 catcttccaa tgttgctacc agatagagaa tgggagcaaa actcaccaaa gaaaagatat
      601 tatagagcaa gcggtaaaga cgcttcatcc cgaacaggtc tttggcgaga tgtgaagcaa
      661 ggaaagaatg tactacgccc cataaagcaa gtgaaagcag gataataaat aggctcatgt
      721 attcttagat gcagcaagag ctggtttcgt tacaaagaat agatagaaaa agcggatgat
      781 attcaaatca tccgcttttt tatactctac aaaattaaga tttaatcgac tccaaaggct
      841 gccagttcgg tcgtctcacc gccagcttgg atgagatagt aaggcttcca agcaatcccg
      901 aagatgttga cgtagatatc ggtcttcttc gggttgatgg tcacttcatt tgactcactc
      961 acgatcctgc cccacttgtc gctaatttcg cgaattactt gctcgcgttc gcggatgagg
     1021 tcattcaagt ctttggtcaa cttggaaatg gtctcctgcg attcttgtac ttcagccttt
     1081 gcactttggg ctaaacggtg cttggtaaat tgagtggtaa cgctcttctt tctcgttagc
     1141 cccaagacac tggcgcccaa ctctaaaaga ttcgctccag actcgatatt gcgattctgc
     1201 aattcggctt cgtcttcgcg cagttcgcgt tcttctctgc ctaatttgtc ttccaacgtt
     1261 ttaatcttct tctcaatggc agaggtcttc ttggcgattt cagtatcgcg ggcttcacga
     1321 gcggtgtcgg cacaagcttt catgaaatct gcctgactca cgtcaggacc cgcaaatact
     1381 ttgagcgcga cattagcacg agcctcaacc gatgaatttc ggaagaccca atcggtaaaa
     1441 tctttttgca aagcagtcat taactttgag tcgttcaaag gcgcttcgat ggaaccgtat
     1501 ttggaggaag gagcgggtcc gctttcgagt ttgtcgagcg aaggaccggc ataggtgaaa
     1561 tcttcccaac ggacaatgcc gcgacggtcg aggctatcta cgagagcggt cttggtcact
     1621 tctacatcca caccgtattt gcggtctaag acgcgaatct gggcagaagc caacaagacc
     1681 ggtttataga ccacgccttg aatattggca ccataagctt ggggttttcc ggcggcgcta
     1741 aaggcttcgg gcatgctgta gttttgcggc aaataatatt cacggatacc agccggaacg
     1801 ggcggcttgg ttagattgcc gccattggaa gattccaaga cagaagacga cgtgctgcgt
     1861 gtgggagtct ttttaggctg tgcaggcgca gaagggactg aaattggttg gacgtggtca
     1921 acaggcggcg gggtggctgt cactttggga gcagagacag gcttcggctt gggttgaggc
     1981 gcagccactt ccccagcgcc gaccaaggcg ttcaagtcgc gaatctgagt gcgggtcatg
     2041 ggacctgcga ggaagttcat cgcccaacgc gtttggaaaa gcacgggatt cttttcgtgt
     2101 acattgttca tgacgaaaac
//
LOCUS       DAOREF010000593         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41180_length_2119_cov_4.002422, whole genome shotgun sequence.
ACCESSION   DAOREF010000593 DAOREF010000000
VERSION     DAOREF010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41180_length_2119_cov_4.002422"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PKI33_11805"
     CDS             <1..924
                     /locus_tag="PKI33_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNM37743.1"
                     /translation="AGLVPVAIGADGGGSIRIPAAFCGLVGLKATFGRVSEYGAFPLD
                     WSVAHIGPIAGTATDVALTYALLAGPDLKDEVSLHQPLPTLEGWDNINLKGLKIGIYR
                     PWFEHAEKEVVDACAAMLKKFEEMGCEIKDIVIPNLEANRVAHTVTIVSEMVQGMSAT
                     YDQHHKEHGLDVRINLALGRKFSADDYVKAQRIRTRMIHHFNNAFKEVDVILTPTTAI
                     AAPEIKKGALPDGDSDLSTTVEIMRFATAGNLTGLPAITFPVGYTQKGLPIGLQAMGR
                     AWDEVTLLRLALASEKVIERKAPQVHFKVLE"
     gene            1072..1467
                     /locus_tag="PKI33_11810"
     CDS             1072..1467
                     /locus_tag="PKI33_11810"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNM37744.1"
                     /translation="MAKKAELKTKKNEASVEDFIETVQDEQVRADCLEIAKMMEQATK
                     AKGKMWGSSIVGFGEQHLVYESGRELDWMKIGFSPRKQNITLYVHLEEGLLKKLGKFT
                     TGKGCLYIKKLADVDKKVLKELIVASATS"
     gene            1470..1760
                     /locus_tag="PKI33_11815"
     CDS             1470..1760
                     /locus_tag="PKI33_11815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM37745.1"
                     /translation="MQKDPEALEKKALHRLVHFQNKHVLEVGCGEGRLTFKYAGSAKQ
                     VTAFDPDHDALRIARADCPPALRPIIHFTGASASHIPYAKETFDIAILAWSL"
     gene            1779..>2119
                     /locus_tag="PKI33_11820"
     CDS             1779..>2119
                     /locus_tag="PKI33_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37746.1"
                     /translation="MVHALSEIHRVLKPNETLLDLRPLEDSWSVEVVSSAGWQAAGRL
                     IDMPQGVADDAAANKAMRDVEASGWFIQKISEEFPYFYSWDTPTEMKAFMENEWEGFE
                     KLDEEVYRTTAS"
BASE COUNT          583 a          476 c          551 g          509 t
ORIGIN      
        1 gcaggactgg tgcccgtcgc catcggtgcc gatggaggtg ggtcgattcg tattccggcg
       61 gcgttttgcg gtttggtcgg cttgaaagct acatttgggc gtgtgagcga atatggcgca
      121 tttccgttgg attggagcgt ggcgcatatt ggtcccattg ctggcacagc cacagatgtc
      181 gcattgacct atgcccttct ggcaggaccc gacctgaaag atgaagtttc tctccaccaa
      241 cctttgccca ccttagaggg ctgggacaat atcaacctca aggggctcaa aatcggtatt
      301 tatcgtccat ggtttgaaca tgcagaaaaa gaagttgtag atgcctgtgc tgccatgctc
      361 aaaaaatttg aagagatggg ctgtgagatc aaagacatcg tcatcccgaa ccttgaagca
      421 aaccgagttg ctcacacagt gaccatcgtc agtgagatgg tgcaagggat gagcgcaacc
      481 tatgaccaac accacaaaga acacgggttg gatgtacgca tcaatcttgc actcgggcga
      541 aagttcagcg cagacgatta tgtcaaagcg cagcgtattc ggacacggat gattcatcac
      601 tttaataatg cattcaaaga agtggatgtc attctgaccc ccaccactgc cattgccgct
      661 ccagagatca aaaaaggcgc tctgcctgat ggcgattctg acttgagcac cactgtggaa
      721 atcatgcgct tcgccaccgc cgggaattta accggcttac ctgctattac cttcccggtt
      781 ggttatacac aaaagggttt accgattggg ctacaagcta tgggacgagc atgggatgaa
      841 gttactctac tacggttggc tttagcttcg gaaaaagtta ttgaacgtaa agctccacag
      901 gttcatttca aggtattgga gtgagttaca gagacattct cgataagctt aaactctctg
      961 tagcaaacgg tcttttattt gccatttcac caaacttttc cattccaaat ttagaacaaa
     1021 taagctaaaa tatatcctat tgtccaaccg agcaaagaaa ggataaaacc catggcaaag
     1081 aaagcagaat taaagaccaa aaagaatgaa gccagcgttg aagactttat agagaccgtc
     1141 caagatgagc aagtgcgcgc cgattgttta gagatagcga agatgatgga gcaagccacc
     1201 aaagcaaaag gcaaaatgtg gggctccagc attgtaggct ttggcgaaca gcatttggtt
     1261 tatgaaagcg ggcgtgagtt ggattggatg aagatcggct tttcaccacg gaaacaaaat
     1321 atcaccttgt atgttcatct tgaagaaggc ttgctaaaga agctcggcaa gttcaccacg
     1381 ggcaagggat gtttgtacat caaaaaactg gctgatgttg ataagaaagt tttgaaagag
     1441 ttaattgttg cctcagcaac ctcatgagtt tgcaaaaaga ccctgaagcg cttgaaaaga
     1501 aagcccttca tcgcctggtt cactttcaga ataagcatgt tctggaggtc ggctgcggtg
     1561 aagggcgctt aacgtttaag tatgccggtt ctgctaaaca agtcacagcc ttcgaccccg
     1621 accatgacgc cttgcgcatc gcccgggcag actgtccccc cgccctgcgt cccatcatac
     1681 attttacggg cgcaagtgca agccatattc cttacgcaaa agagacattt gatatcgcca
     1741 tcctcgcttg gtcactctga tgactggcac atgagagcat ggttcatgct ctgagtgaaa
     1801 ttcaccgggt acttaagccg aacgagactc tgcttgattt gcgtccattg gaggattcgt
     1861 ggtcggtgga ggtggtttca tcggcgggct ggcaggcggc ggggcgtttg atcgatatgc
     1921 ctcagggggt agctgatgat gcggcagcga ataaagccat gagagacgta gaggctagcg
     1981 gatggttcat ccaaaaaata agtgaagagt tcccatactt ttattcttgg gacacgccca
     2041 cagagatgaa agcgtttatg gaaaacgaat gggaggggtt tgagaaattg gacgaagagg
     2101 tataccgcac gactgcctc
//
LOCUS       DAOREF010000594         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41222_length_2118_cov_4.679593, whole genome shotgun sequence.
ACCESSION   DAOREF010000594 DAOREF010000000
VERSION     DAOREF010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41222_length_2118_cov_4.679593"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKI33_11825"
     CDS             <1..211
                     /locus_tag="PKI33_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37747.1"
                     /translation="GIVSLPAPWIGAQLWARFGPTAPFKITATVILLSIIPIWLKFKL
                     PKTTEETQPEADQPVEENKVAIAAG"
     gene            252..638
                     /locus_tag="PKI33_11830"
     CDS             252..638
                     /locus_tag="PKI33_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37748.1"
                     /translation="MANVEERMKILKLIEEGKISAEEGAKLLSALNDSRKNIPTPPRP
                     PSAPGGARMLRVRVTDTRTGRSKASVQIPLALVDAGLKIGAHFAPEVQGVDMSNVMEA
                     LRMGVTGKIIDVTDEEDGEHVEIFVE"
     gene            790..>2118
                     /locus_tag="PKI33_11835"
     CDS             790..>2118
                     /locus_tag="PKI33_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM37749.1"
                     /translation="MQETKTNRPSGMFAFTIVWLGQIISVLASAMSQFGLTIWMYEKT
                     QSATALGLMQVFFITPFLIISPIAGVMVDRHNRKLMMMVSDLTAGLATILILIFQSLG
                     VLQFWHLYVASIIYGLGNAFQWPAYSAAISTMVPKDQYGRANGMMSLIEAGPGVFAPL
                     LAGALLPLIGLTGILVIDVVTFSFAVGALLIVHIPQPERTVEGVQGKGSVWQEAVFGF
                     KYIFARPSLLGLQMVFFFGNLFSGILFTLLAPMILARTDNNSLMFGTVQTAGAIAGVV
                     GGLIMSAWGGFKRKVHGVLGGWAITGLGSAVIGLSFGLPAWVIGVAIASLISPLVNAS
                     NQAIWQSKVAPDLQGRVFSARRLIAWFTNPISPIVAGTLADYVLEPQMRAGGTLSQTF
                     SWLVGTGPGSGMGLIILACGVFCVLIGVAGYFIPAIYNAETILPDHDQLAK"
BASE COUNT          478 a          457 c          570 g          613 t
ORIGIN      
        1 gggtatcgtg tcgcttccgg ctccgtggat tggggctcag ttgtgggctc gctttggtcc
       61 aaccgccccg tttaagatca cagctaccgt cattttgctt tcgatcatcc cgatctggtt
      121 aaaattcaaa cttccgaaaa caactgaaga aacccagcca gaagcagatc agccagtaga
      181 agaaaataaa gtcgctattg cggcaggata aaataagcgt tcgtcctgaa cggcgaaagg
      241 agaaatggaa catggcaaac gtagaagaaa gaatgaaaat tctgaaatta attgaggaag
      301 gcaaaatcag tgcggaggaa ggcgcgaaac tgctctcagc tttgaacgac tcgcggaaga
      361 atattcccac gcctccgcgc ccgccttctg ctcccggtgg tgcacgcatg ttgagagtcc
      421 gcgtgacaga tacccgcact gggcgttcca aagcctctgt tcaaattcca ctggcattag
      481 tggatgccgg gttgaagatc ggtgctcatt tcgctcctga agttcagggt gtagacatga
      541 gtaatgttat ggaagcactc cgcatgggtg ttaccggcaa gatcattgac gtgaccgatg
      601 aagaagatgg tgaacacgtc gagatctttg tcgaataaag tctgtcggtt cgtgttggtt
      661 atcagaagga gaaaactatg ttcccatcac tataccttgt ccatcgcttg atgttatagc
      721 catcaacgcg caagagattt ctcttgcgcg ttgtatcatt tttatcacag atcatcaaag
      781 gatactttta tgcaagagac aaagacaaat cgccccagtg gtatgttcgc atttacgatt
      841 gtgtggctgg ggcagatcat ttccgtgctt gccagtgcga tgagtcaatt tgggcttacg
      901 atctggatgt acgaaaagac ccaaagcgct actgcgctgg gtttgatgca agtcttcttc
      961 attaccccat tcttgattat ttctcccata gctggtgtga tggtagaccg gcacaaccgt
     1021 aagttgatga tgatggttag cgacttaacc gctggacttg ctacgatttt gattttaatc
     1081 tttcagtcgt tgggtgtttt gcaattctgg catttgtatg tagcttcaat catttatggg
     1141 ttgggaaatg ctttccaatg gcccgcatat tcggctgcaa tttccacgat ggtgccgaag
     1201 gatcagtacg gtcgcgcaaa cggcatgatg tccctaatcg aggctggacc cggcgttttt
     1261 gccccgctat tagccggggc gttgctgcca ttgatcggtt tgactggcat ccttgttatt
     1321 gatgttgtga cattttcatt tgccgttggt gccttgttga tcgtccacat ccctcaacct
     1381 gaacgaacgg tggaaggtgt tcagggaaaa ggcagtgttt ggcaggaggc ggtttttggt
     1441 ttcaaatata tttttgcccg ccccagcctg cttggtttgc aaatggtgtt tttctttgga
     1501 aatttatttt cgggtattct gttcacttta ttagcgccca tgatccttgc aagaaccgat
     1561 aacaacagct taatgtttgg tacggttcaa acggcaggag caattgctgg tgtagtgggt
     1621 ggcttgatta tgagtgcctg gggcgggttt aaacgcaagg tacatggcgt gttaggtgga
     1681 tgggcaatta cagggcttgg gtctgctgtg attggcttga gttttggtct gcctgcatgg
     1741 gtgattggcg ttgctattgc ttcgttgatt agtccgcttg tcaatgcatc taatcaggcg
     1801 atttggcaat caaaggtggc accagatctt caaggacgtg ttttttccgc acggcgtttg
     1861 attgcttggt tcacaaatcc catttctccc attgttgctg gaacactggc ggattatgtc
     1921 cttgagccgc aaatgcgagc cggtgggacg ctttcgcaga cctttagctg gttggtgggc
     1981 accggtcccg gttcagggat ggggctgatc attttggcat gcggtgtgtt ttgcgtcttg
     2041 atcggcgtag cggggtattt catccctgca atttacaatg cagagacgat cttgcctgac
     2101 catgatcaac tggcaaaa
//
LOCUS       DAOREF010000595         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41232_length_2118_cov_3.144935, whole genome shotgun sequence.
ACCESSION   DAOREF010000595 DAOREF010000000
VERSION     DAOREF010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41232_length_2118_cov_3.144935"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKI33_11840"
     CDS             <1..356
                     /locus_tag="PKI33_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM37750.1"
                     /translation="SGGEAQRILLARALCQSTPILLLDEPTAHLDLQYQVSLLELIRE
                     LAHKENLAVLIALHDLNLAARYTDRVALLVGGRLNAIGTPREVLTPEKISNAYCLPVN
                     VVEHPYMDAPLVLPK"
     gene            complement(395..1156)
                     /locus_tag="PKI33_11845"
     CDS             complement(395..1156)
                     /locus_tag="PKI33_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNM37751.1"
                     /translation="MLTPLDYLFAALAALAAGAINALAGGGTLITFPVLTFLGIPAVS
                     ANITNTVALCPGYFGGTMAQAKDLKDQTKRLWMLIPASILGGILGGFLLLQTGEKLFK
                     DLVPYLILLACLLLAIQDPVRAWLTRRMAEGQGATLEQWTWLPVGLASVYGGYFGAGL
                     SVIVLSALGLTLEDSLTRLNALKQAVGFAVNVAAAVFFLFSGQVIWSVALVMAVGALI
                     GGNLGGRLAGKIKPSTLRWTVVTIGVIVAIIYFVR"
     gene            1268..>2118
                     /locus_tag="PKI33_11850"
     CDS             1268..>2118
                     /locus_tag="PKI33_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37752.1"
                     /translation="MFTNKPSRFSQVILFLGVMFSIAWSLYFEKSILATPYEIGYREG
                     APQVITQMMIDRKNFSTFEYQPLGYNAYGLGYHLVVLPFALIFGNTLHVHRLISFIFI
                     LLSAAISFWIVFQQKRNFLIAAICSAFIMVALMGWGGLGSAPTTMGAFLFLAATFIPI
                     LFSFEDKSLIASAVLAVFAFYTKAYFVLSFGIVAAYLFLFVSKKKAINYGLSFLVPFV
                     LLFVAIRISYPLYFINVILGNASNTFRTFEHLYTQLFWLTVYFFPTLVLAGIMLWRSI
                     NKKNNTP"
BASE COUNT          576 a          516 c          437 g          589 t
ORIGIN      
        1 tctctggcgg tgaagcgcaa cgcatcttac tcgcccgcgc cttatgccaa tctacaccca
       61 tcctcttgct cgatgaaccc accgctcatt tagaccttca atatcaagtc agtttattgg
      121 aattgatccg cgaattggcg cataaagaaa acctcgctgt gttgattgct ttacatgatc
      181 taaatcttgc cgcccgttat acagaccggg ttgctttatt ggttggcgga agactcaatg
      241 ccattggcac tccacgcgaa gtgctcaccc ccgaaaaaat ttcaaatgct tattgcctgc
      301 ctgtaaatgt tgtggagcac ccatatatgg atgctccact ggtattgcca aagtagttta
      361 atgtcattgc aagagcgcgg ctcgcaatga cgaatcaacg cacaaaataa atgatcgcca
      421 cgatcacgcc aatcgtcacc accgtccatc gtaatgtgga cggtttgatt ttgccagcca
      481 aacgtccgcc caagtttcca ccgataagcg cgcccaccgc catcaccaac gccaccgacc
      541 agatcacctg acccgagaat aggaagaata ctgccgccgc cacgttaact gcgaatccaa
      601 ccgcttgttt gagcgcgttc aaacgggtca gcgaatcttc taaggtcaag ccgagagccg
      661 agagaacgat cacactcaag cccgcaccaa aatatccgcc gtagaccgag gcgagcccaa
      721 ccggtaacca cgtccactgc tctaatgtag ctccctgccc ttctgccata cgcctcgtca
      781 accatgcacg gacggggtct tgaattgcca gcaaaagaca agcaagcaaa atgagatacg
      841 gcacaaggtc tttgaataat ttctcaccgg tttgcaaaag caaaaatcca cccagaatgc
      901 cacccaaaat actagcgggg ataagcatcc acaagcgttt ggtttggtct ttcaagtctt
      961 ttgcttgagc catggtcccg ccgaagtaac cggggcaaag tgccaccgta ttcgtgatat
     1021 ttgcagagac agcggggata cccagaaagg tcaagacagg gaaggtaata agcgtcccac
     1081 caccagctag ggcgttaatc gctcctgcag caagcgcagc aagagcagcg aagagataat
     1141 cgagaggtgt gagcatgagg ggtgttttcc ttttgagaga ttataaactg cacgagcggt
     1201 atggtagagt tatgagtgcc aatttcctat aaaaaatatt ttgaatgaat ccagtaaaat
     1261 agttcatatg tttacgaaca aaccttctcg tttctcgcaa gttatattgt ttttaggggt
     1321 aatgttttca atagcatggt cactttattt tgaaaagtca attcttgcca ctccttacga
     1381 aataggatat cgggaaggcg cacctcaggt catcacgcaa atgatgattg acaggaaaaa
     1441 tttttctacg tttgaatatc agccactggg atacaatgct tacggattag gatatcacct
     1501 agttgttctg ccattcgcat tgatatttgg caacacctta catgtccaca gattaatttc
     1561 ttttatcttc attttgctat ccgctgcaat cagcttctgg atcgtatttc agcaaaaacg
     1621 caacttcctt atcgctgcaa tttgcagtgc attcatcatg gtcgcattga tgggctgggg
     1681 agggttaggt tctgctccga ctaccatggg agcattctta tttttggcag cgacattcat
     1741 ccctatttta ttttcttttg aggacaaaag tctgattgca agtgccgttc ttgccgtgtt
     1801 tgcattttac acaaaggcat attttgtact ttcctttggg atcgtagcag catacctttt
     1861 tctttttgtt tccaagaaaa aagcaataaa ctatgggtta tcttttctcg tcccattcgt
     1921 tttattgttt gtagctattc gcatctctta ccccctctat tttattaatg ttatattagg
     1981 aaatgcatca aacacattca gaacttttga gcatctttac actcagcttt tctggttaac
     2041 agtctacttt tttccaaccc ttgtattagc aggcattatg ctttggagaa gtattaacaa
     2101 aaaaaacaat acaccaga
//
LOCUS       DAOREF010000596         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41271_length_2117_cov_4.464113, whole genome shotgun sequence.
ACCESSION   DAOREF010000596 DAOREF010000000
VERSION     DAOREF010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41271_length_2117_cov_4.464113"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..902
                     /gene="rlmB"
                     /locus_tag="PKI33_11855"
     CDS             177..902
                     /gene="rlmB"
                     /locus_tag="PKI33_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNM37753.1"
                     /translation="MKEFIYSRNAVHEALLAKRRDIFSIEIAEGAQEKGTLAEIIKIA
                     QQRKIKLFRSPRPKLDKVNQHHQGVVAEVSGYPYADVVDILDKPKGEPPFILILDSLQ
                     DPQNFGTLIRTAEAVGVHGIIIPLARSVEVTPSAVNASSGASEHMLIAKSNLSQAMDA
                     LRDADIWLVGLDQAGAEVEANSRHLKGALGLVVGSEGEGIHELTRKKCDIVLKLPMKG
                     KIESLNAAVAGSVALYLAYLNRK"
     gene            952..>2117
                     /locus_tag="PKI33_11860"
     CDS             952..>2117
                     /locus_tag="PKI33_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNM37754.1"
                     /translation="MPQGTYHFPKGFLWGTATASHQVEGNNTNNNWWKWEQEGHTNGS
                     AAIAADWWGGRWREDFDRAAETGQNAHRLSIEWSRVQPTPDRWDEDAIERYRTMLRGL
                     RDRNMTALVTLHHFTDPLWVTERGAWETEEIVPLFEKYVRKMVDSLKEYTNLWVTINE
                     PNVYALSGYVTGDFPPGKKDLKQAMPVIANMVRGHAAAYRAIHELQPEARVGYALHFR
                     PMVPRLSWSPLDRLMRNIRYEGVNMGFPTAISTGVMKTPIGNFNIPEAKGTQDYLGLN
                     YYSVDTVWFDLAKADQMFSNSGYPKDADMSDTNFLANIPHGIYDSIKWIVRTYPNLPI
                     IVTENGVEDFDDHMRPRYLVQHLHEVWRTVNFNWPVKGYFHWSLVDNFEWERGWT"
BASE COUNT          546 a          488 c          585 g          498 t
ORIGIN      
        1 ggcgagtgaa acaagcaaaa gcaataccag taaaagagac tttttgtttt tcattagaac
       61 tccttgagat aattgaattc tatcgcttaa cttaattagt gtttggttcg gaatgtttag
      121 tgtcctcctg cggtttgata taaaacaagc gtacatcaag taaaatacac tttgatatga
      181 aagaattcat ctattcacgc aatgccgtcc atgaagcctt gcttgccaaa cgcagggaca
      241 ttttttcaat agaaattgcc gaaggggcac aagaaaaagg cacactggca gagatcatca
      301 aaattgcgca acagagaaag atcaagctat tccggtcgcc gcgccccaag ttagacaagg
      361 tcaaccagca ccatcaaggt gtggtggcag aggtgagcgg ctatccctat gcagatgtgg
      421 tcgatattct ggataagccc aaaggcgaac cgcctttcat cctcattctt gactcgttgc
      481 aagacccgca gaacttcggc accctcatcc gcactgcaga agcggtggga gtgcatggca
      541 ttatcattcc gctggcgcgc tcagtggagg tgacaccctc tgcggtgaat gcttcttcag
      601 gcgcgagtga gcatatgctc atcgccaagt cgaatctttc gcaggcaatg gatgcgcttc
      661 gagatgcaga catttggctg gtagggttgg atcaagccgg ggcggaggtg gaggcgaatt
      721 cccgtcatct caaaggagca ttgggattgg tcgtcggctc ggagggtgaa ggcatccacg
      781 aactgacgcg caagaagtgt gacattgtgt tgaagttgcc gatgaagggc aaaattgaat
      841 ctttgaatgc tgctgtggca gggtcggtgg cattgtattt ggcgtatttg aatagaaaat
      901 aatgtgtagg ggcgacttgc aagtcgcccc tacgatacta tggagatcat catgcctcaa
      961 ggcacttatc attttccaaa agggttttta tgggggacgg cgactgcttc gcatcaggta
     1021 gaaggcaata acacaaataa caattggtgg aagtgggagc aggaaggtca caccaatggg
     1081 tcggcggcaa tcgctgccga ttggtggggc ggacgttggc gcgaagattt cgaccgtgca
     1141 gccgagacgg gtcagaatgc acatcgtctg tccattgagt ggagccgtgt gcagcccaca
     1201 cctgatagat gggatgaaga cgccatcgaa agatatcgca ccatgttgcg cgggctgcgt
     1261 gatcgcaata tgaccgcctt ggtgacgtta catcacttca ccgacccgct gtgggtgacg
     1321 gaacgtggcg catgggaaac cgaagagatc gtgccgttgt tcgagaaata tgtgcgcaag
     1381 atggtggatt ctttgaagga atacaccaac ctatgggtga cgatcaacga accgaatgtc
     1441 tatgcgctaa gcggatatgt aacgggagat ttcccgcctg ggaaaaaaga cttaaagcaa
     1501 gccatgccag tcatcgccaa tatggtgcgc ggacatgccg ccgcctaccg tgccattcat
     1561 gagttgcagc cagaagcacg ggtggggtat gccttgcatt tccgcccgat ggtgccgcgt
     1621 ttgagttggt cacctttaga ccggctaatg cgcaatatcc gctatgaagg cgtgaatatg
     1681 ggcttcccca ctgcaatctc taccggtgtg atgaagactc caatcggcaa tttcaacatc
     1741 cctgaagcca aagggacgca agattatctg ggcttgaact attactccgt ggatacggtt
     1801 tggttcgatc ttgcaaaggc agaccagatg ttttcaaaca gcggctaccc caaagatgcc
     1861 gatatgagcg acacgaattt tctcgcgaat attccgcatg gcatttacga ctccatcaaa
     1921 tggatcgtgc ggacctatcc aaacctgccc atcatcgtca ctgaaaacgg cgtggaagat
     1981 ttcgacgatc acatgcgtcc gcgctatttg gtgcagcatt tgcatgaagt ttggcgcacg
     2041 gtcaatttca actggcccgt gaagggttac ttccactggt cactggtgga taactttgaa
     2101 tgggagcgcg gttggac
//
LOCUS       DAOREF010000597         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41369_length_2114_cov_4.533754, whole genome shotgun sequence.
ACCESSION   DAOREF010000597 DAOREF010000000
VERSION     DAOREF010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41369_length_2114_cov_4.533754"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKI33_11865"
     CDS             <1..224
                     /locus_tag="PKI33_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNM37755.1"
                     /translation="AAGVEKVKVVSDVLEAEFGEKYGILIKERRYLRRSVFIVGRDGK
                     LTYVNYLPALGDEPNYEEVIAAAKDALKA"
     gene            complement(392..1951)
                     /locus_tag="PKI33_11870"
     CDS             complement(392..1951)
                     /locus_tag="PKI33_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNM37756.1"
                     /translation="MANNLIGKTISNRYVIEEMLGQGGMSAVYKGNDPNLKRVVAIKV
                     IHSHLSGNPDFVQRFEEEAAAVAQLRHPGIIQVYDFNRDDDLYYMILEFVPGETIQDH
                     LKRLNDSGRKLSPTKAAEYMAGICDAVDYAHQRGMIHRDIKPANLMLTITGQVILMDF
                     GIAKIVGGTRHTATGAVVGTAMYMSPEQIKGEQPDRRTDIYSLGVTLFEMVSGRPPFE
                     AESAMTLMMMHINDPVPDPKKLNPDVPDALVAVIQKALAKDPNNRYQSAAQMAAALRN
                     LLSAGTSSPLATMMEDATIMNSSRGTMIEKPYAGTYVESKPIAPAPGTMNKNSAPSSM
                     YATPAPAASAPKRKNLLLLPIIAVVAIGLICLVGGVAFAAKQLFGGVVETEPPVVLAT
                     NTQPAIVAPIETEVSTETPIPATPTPSSPYVVITAIRVEGSLYAVDYDVHNFTDGLHV
                     HIFFNTVPPEQAGSPGSGPWKLTWGAYGNPPFTQYSIAARPANATQMCALVANSNHSV
                     QPNSGNCVDLP"
BASE COUNT          547 a          464 c          511 g          592 t
ORIGIN      
        1 gcgcagctgg tgtggagaag gtcaaagtag tgtcagatgt attggaggcg gagttcggcg
       61 aaaaatacgg catcctcatc aaagaacgcc gctacctgcg ccgttctgtc ttcatcgtcg
      121 ggcgagatgg aaaattgact tacgtcaatt acctgcctgc actgggtgat gaaccaaatt
      181 atgaagaggt catcgcagcg gcaaaggatg cattgaaggc ataaagaaca aacacccttt
      241 tgcaagggtg tttgttcttt tatatgcaat agtttgttta tgccgttaca gcgggtctaa
      301 atttatacac gtatatgtga tatataccgt aaagttactt gagagcaaag gcgaaaacct
      361 tgactgtaaa taactctacc aagagttact tttacggcaa atctacgcag tttccagagt
      421 ttggctgaac agagtgattc gaattggcaa ctaatgcaca catttgagtt gcgttcgccg
      481 gtcgagcggc aatgctgtat tgagtgaagg gtgggttgcc atatgccccc caggtcaact
      541 tccagggacc tgatccaggc gagcctgctt gttccggtgg aacagtgttg aaaaatatat
      601 gcacgtgcaa tccatcagtg aagttgtgta catcataatc cacagcgtaa agacttcctt
      661 cgactctgat tgcggtgatg actacgtagg ggctcgaagg ggttggtgtt gccgggattg
      721 gagtttcagt tgagacctct gtttcaatag gagctacgat tgcgggttga gtattggtcg
      781 cgagtacaac cggcggctct gtttctacaa cacctccaaa gagctgcttg gctgcaaaag
      841 caactcctcc aacaagacaa attaatccaa tagccactac ggcaatgatc ggcagaagta
      901 acaaattttt tcgctttgga gcagaagccg ccggcgccgg ggttgcgtac atcgaacttg
      961 gcgctgaatt tttgttcatt gttccaggtg caggagcgat aggtttactt tccacatatg
     1021 tgcctgcata tggtttctct atcattgttc cacgcgatga attcataatg gtggcatctt
     1081 ccatcatcgt ggcgagtggt gaagatgtgc cagcggaaag caaatttctt agagcggctg
     1141 ccatttgagc agcgctctga taacgattgt tcggatcctt cgcaagcgct ttttggatga
     1201 ctgcgaccaa agcatctggc acatcaggat tgagcttttt gggatccggc accggatcgt
     1261 ttatatgcat catcatcaat gtcatggctg attcggcttc aaaaggcgga cgtccgctga
     1321 ccatttcaaa caaggttacg ccgagagagt aaatatctgt gcgccggtcg ggttgttctc
     1381 ctttgatctg ttcaggcgac atatacattg cggttccgac aaccgccccg gttgctgtgt
     1441 gacgtgtacc gccaacgatc ttggctatgc cgaaatccat cagaataact tgacctgtta
     1501 tggtaagcat taagttagct ggtttaatat cgcggtggat cattccacgt tggtgcgcat
     1561 aatcgactgc atcgcaaatg cctgccatgt attcagcggc tttggtgggg gagagttttc
     1621 taccgctatc gttgagtctt ttcaagtgat cctgaattgt ttctccgggc acaaattcca
     1681 aaatcatata ataaaggtca tcatcgcgat tgaagtcata gacttgaata ataccgggat
     1741 ggcgtaattg tgctactgct gcagcttcct cttcaaaacg ctggacgaag tcagggttgc
     1801 ccgataaatg ggagtgaatg accttgatag ccacaacacg ctttaagttg gggtcattgc
     1861 ctttgtaaac tgcagacatc ccaccttgcc ccaacatttc ttcgataaca tatcgattgc
     1921 taatggtctt tccaattaag ttattagcca ttgctactcc tagaaaataa aaacattgac
     1981 actcttaaat aatattcgta atactgaaat aacgctcagc tttataccct actttaatcc
     2041 ttatggtgaa tctatgagaa aaagccatgt gttatcgaac ttacaattgg tacttcgccc
     2101 tgattcgctt aacg
//
LOCUS       DAOREF010000598         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41447_length_2112_cov_4.704424, whole genome shotgun sequence.
ACCESSION   DAOREF010000598 DAOREF010000000
VERSION     DAOREF010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41447_length_2112_cov_4.704424"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKI33_11875"
     CDS             complement(<1..1099)
                     /locus_tag="PKI33_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HNM37757.1"
                     /translation="MNPIIIILIDILALLVGAVAGYFFHRYQVDRAAKARQERADDIL
                     KAAQTQANMIVKGAQDNATKIIQAAESELKERRIELNRDTERLEKRRSEVDSRYDKIE
                     QREQNLNRRQSQIDKRHNEAEKLFEDQMKKLEEIAQMTQDEARKDLFAAVEKEARGDM
                     ARIIRQIEAEAREEGEKRARKLIADAIQRVASEHVAEVTRAVVTLPSEEMKGRIVGRN
                     GRNIKAFEQAAGVDVIVDDTPDSVTISCFDSVRREIARRALSKLILDGRIHPAHIEKV
                     IDDETRAVEKIINEAGEQAAYDANVSGLHPEILRMMGRLKFRTSYGQNQLAHAVEVSK
                     LAGILAAEIGANVELAKLGGFLHDIGKAMVHN"
     gene            complement(1102..1749)
                     /locus_tag="PKI33_11880"
     CDS             complement(1102..1749)
                     /locus_tag="PKI33_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNM37758.1"
                     /translation="MRRITALEVQRKNPNRVNIYLDGEFAFGLARITAAWLKVGDILT
                     DEKITALQNEDARERALQQALLFLSYRARSEKEIRQNLKKHEYPEDAIEYTMTRLREN
                     RLANDNEFAQAWVENRNTFRPRSRRALTMELRQKGLDEETVKNAVADIDENALAYETA
                     RKRAPRLKSLEWSEFRKKLSEYLARRGFPYSVVSSTVTRIWNEAHTEETKYEDEE"
BASE COUNT          452 a          513 c          538 g          609 t
ORIGIN      
        1 gattgtggac cattgctttg ccgatgtcat gcaagaaacc gcccagtttg gcgagttcca
       61 cattggcacc gatttcagca gcgaggatgc cagccaactt ggaaacttcc acagcatgag
      121 caagttgatt ttgaccataa gaagtacgga acttgagacg acccatcatg cgcagaattt
      181 cggggtgtaa accagagaca ttcgcatcat atgcggcttg ttcaccggct tcgttgatga
      241 tcttttccac ggcgcgggtt tcatcatcaa tcactttttc aatgtgagcc gggtgaatac
      301 gtccatcgag aatcagtttg gaaagagcgc ggcgagcaat ttcgcggcga acggagtcaa
      361 aacacgagat ggtgacagag tctggcgtat cgtccacaat cacgtccaca ccagcggctt
      421 gttcgaaggc tttgatgttg cgtccgttac gaccaacgat acgtcccttc atctcttcgc
      481 tgggcaaggt aactacagca cgagtaactt ccgccacatg ttcagatgcc acacgttgaa
      541 tggcatcggc gatgagcttg cgggcgcgct tttcgccttc ttcccgagct tcggcttcga
      601 tctgacggat gatgcgagcc atgtcgccac gggcttcttt ttcaacagca gcgaagaggt
      661 ctttgcgcgc ttcgtcttga gtcatctgcg cgatctcttc gagctttttc atttgatctt
      721 cgaacaattt ctcggcttcg ttatggcgct tgtcaatttg tgattgacgg cgattgagat
      781 tttgttcgcg ttgttcgatc ttgtcgtagc ggctgtccac ttcagagcgg cgtttttcga
      841 ggcgctcggt atcgcggttc agttcgatac ggcgttcctt gagctcactt tcagcagcct
      901 gaatgatctt ggtggcatta tcttgcgcac ctttaacgat catattcgcc tgggtttgtg
      961 cagctttcaa aatatcatca gcacgttcct ggcgtgcttt ggcagcacgg tctacttgat
     1021 agcgatggaa gaaatacccc gctacggcac caactaaaag tgccagaatg tctatcaaaa
     1081 ttatgatgat cggattcatg ttcattcctc atcttcgtat tttgtttcct ctgtgtgcgc
     1141 ctcgttccaa atacgggtga cggtagacga aaccacagag taggggaagc cgcgccttgc
     1201 aagatattcg gatagtttct tacgaaactc gctccattcc agacttttga gtctgggtgc
     1261 ccgttttcgg gctgtttcat acgccagggc attttcgtct atatcggcaa cggcgttctt
     1321 gaccgtttcc tcatcgagcc ctttttgacg tagttccatc gtcagtgcgc ggcggctgcg
     1381 cgggcggaat gtgttgcgat tttcgaccca cgcctgagca aattcattgt cattggctaa
     1441 cctgttctct cgcagacggg tcatcgtata ttcgatggca tcttcgggat attcatgctt
     1501 tttcaggttt tgccttattt ccttctcgga acgagcacga taactaagaa acaagagtgc
     1561 ttgttgcaaa gctcgttcgc gcgcatcttc attctgtaag gcggtgattt tctcgtcggt
     1621 gagaatatcg cccactttga gccatgcggc tgtaatgcgt gccaacccga aggcgaattc
     1681 cccgtcgagg taaatgttta cccgattggg gttcttccgt tgtacttcga gagcggttat
     1741 ccttcgcatt ttgttttttt gcgtgttttg cccctcgaaa ttgagagcga gtagtcttaa
     1801 acacgcacag ccgaggcttc cgacagaatc ggtctcggct gtgaaaaggt ctggggttgg
     1861 gtaccaggct ctggttccaa aaaagaggcg ttgccagaga taggtcgaaa tccgtggcgg
     1921 gattccgtca cgttcggcac ttgtagattt taaggtgaag ccagttggct caagttcgta
     1981 tttctattgt tggacttcac aagttaactc ccaaaacctg gggaggatcg tgcgcgaatt
     2041 ttccagagaa tttcaagcaa aagaccgcaa agcggaaggt gtcttaagtt ttcttaatta
     2101 tacatgaatt tt
//
LOCUS       DAOREF010000599         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41477_length_2112_cov_2.269324, whole genome shotgun sequence.
ACCESSION   DAOREF010000599 DAOREF010000000
VERSION     DAOREF010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41477_length_2112_cov_2.269324"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..830
                     /locus_tag="PKI33_11885"
     CDS             78..830
                     /locus_tag="PKI33_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNM37759.1"
                     /translation="MFFGFDLKEIFYFPFKDAEARKFLLIGTLVSLAGFIVPILPYFL
                     MTGYAVQIVRQIFRNETPRMVAWDNWNDLFKDGIKVFGVRLLAVLPILVLVLPIMVTS
                     ILLPIFTGNSANPEADPFFAVFMGIFGLSMCIIIPFSIFVAVVIPAAEMHVADSDDFK
                     AAFRFREWWGIFRANLSGFLAAFAIYYLAGMAIGILVQILMVTVVLACLLPIVLPAVA
                     FYLYIIMYVTGAQAYRDGKAKLAQTEAAVTAA"
     gene            complement(865..>2112)
                     /locus_tag="PKI33_11890"
     CDS             complement(865..>2112)
                     /locus_tag="PKI33_11890"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNM37760.1"
                     /translation="ILSFSNARWYDRRIYALLFSFASIGMLWVLLQAGELNVLLQIVV
                     YNVLLFLACMICHGELYRLRPDAGHLTSFYLMVSVGGAVGGLFVNLVAPFIFTGYWEF
                     YLAWLLTIVLLVVMLFPRFSARVDLQSRSMSLAFIFGIVLLALGLNPYQNALFAERNF
                     YGVIRVRAYDSNSLAMIHGATVHGIQILQERDRPTTYYVEESGVGLLLRNHPKRGNMN
                     VGVLGLGIGTVATYGMPGDNYRFYEINPVVVRLAEGEGDYFSFLSDSQAKVTVIEGDA
                     RISLEKELADEAILPFDVLVLDTFSSDSIPVHLVTREAFSLYIQHLTPDGVIAAHISN
                     RHLDLQPVFWQLAQEYGLKMVRITNEAGAEEEFPSEWLLLTSNEDILNIPQIQEHALG
                     YEGYTTSIQIWTDDYSNLFQILR"
BASE COUNT          582 a          516 c          451 g          563 t
ORIGIN      
        1 tcgttctact cgctgcggct gcacattttg tggctgtgat gggtgtggcg ctctactaaa
       61 ctagtaaagg aactttcatg ttttttggtt ttgacctcaa agaaatcttt tacttccctt
      121 tcaaagatgc ggaagcacgg aaatttctgc taatcggcac attggtctct ctggcaggat
      181 ttattgttcc cattctgccg tacttcttaa tgaccgggta tgcagttcaa atcgttcggc
      241 agattttcag gaatgaaact ccgcgtatgg ttgcatggga taactggaat gacctattca
      301 aggacggcat taaggtattc ggggtccgtc ttctggcagt tttgccaatt cttgtgcttg
      361 ttttaccgat catggtgacg tccattctgt tgccgatctt cacagggaat tcagccaacc
      421 ccgaagcaga cccgttcttt gcggtgttca tgggcatctt tgggttaagc atgtgcatca
      481 tcattccatt ctcgatcttt gtagctgtag ttatccctgc cgcagagatg catgtagcag
      541 atagcgacga ctttaaagcc gccttccgct tccgtgaatg gtggggaatc ttccgcgcca
      601 acttaagtgg attcctagct gcctttgcca tttactatct ggctggcatg gcaataggta
      661 tccttgtaca aatcctaatg gtcacggttg tgttggcatg tttgttacca atcgtattgc
      721 ccgcagttgc tttttacctc tacatcatta tgtacgtcac aggtgcgcaa gcgtaccgag
      781 acggcaaagc gaaactagct caaaccgaag ctgccgtcac tgcagcctaa caaaaaagcg
      841 gacacttcag tgtccgcttt tttgttatct caaaatctga aataagttac tgtaatcatc
      901 cgtccatatt tgaatggagg tggtatagcc ttcgtacccc aaagcatgtt cttgaatttg
      961 agggatgttc aaaatatctt cgtttgaggt taacaatagc cattcagaag ggaattcttc
     1021 ttcggcaccg gcttcattcg taatgcggac catcttcaaa ccgtattcct gtgccaattg
     1081 ccaaaaaacc ggctgaaggt ccaaatgacg atttgagatg tgagccgcaa tcacaccatc
     1141 gggtgtaagg tgttgaatgt atagcgaaaa ggcttctctt gtcaccaaat ggaccgggat
     1201 cgagtcgctg ctgaatgtat ccaaaaccag cacatcgaac ggcagaatgg cttcgtctgc
     1261 caattccttt tcaagcgaaa ttcgtgcatc accttcaata actgtcactt tcgcctgact
     1321 atcggataag aagctaaagt aatctccttc gccttcagcc aaacgaacca caaccggatt
     1381 aatttcatag aaccggtaat tatcgcctgg cattccatat gttgctaccg tgccaatccc
     1441 caagcccaat acaccaacat tcatattgcc acgtttggga tggtttctca gcaacagccc
     1501 gactccgctt tcttctacat aatatgtagt tgggcgatca cgctcttgca gaatttgaat
     1561 tccatgcaca gtggctccat ggatcattgc caaagaattc gagtcgtatg cacgcacgcg
     1621 gatgacccca tagaaattcc gctcggcaaa caaagcgttt tgataggggt ttaaacctag
     1681 cgccaaaagc acgatcccaa agataaacgc caaactcatg gagcgagatt gcaaatccac
     1741 ccgggctgaa aaccgtggga ataacattac aaccaataag acgatggtta gtaaccaagc
     1801 caaataaaat tcccaatatc ctgtaaaaat aaaaggcgct actaaattga caaatagtcc
     1861 acccactgcg ccgccaactg acaccattaa ataaaaactg gtgagatgcc cagcatctgg
     1921 gcggaggcga tatagctcgc catggcaaat catacaagcc aaaaagagca aaacgttgta
     1981 tacgacgatt tgcagcagca cattcaactc accggcttgc agcaaaaccc ataacatacc
     2041 tatactggca aaactaaaca atagcgcata tatacgacgg tcataccatc gcgcattaga
     2101 gaatgacagg at
//
LOCUS       DAOREF010000600         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41560_length_2109_cov_4.387050, whole genome shotgun sequence.
ACCESSION   DAOREF010000600 DAOREF010000000
VERSION     DAOREF010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41560_length_2109_cov_4.387050"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKI33_11895"
     CDS             <1..453
                     /locus_tag="PKI33_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37761.1"
                     /translation="YERVGFTKIRRLVGYKLENPQVESDVELEEIDIRELARLVAYHG
                     LKDLPWQLSGATVMQHTPPSRAFHVNDAYCLITNPEATDIAISSVLVKTRSRGAGLSA
                     VLLRALFAKFPNKVWHVSPIYPEEMGVIFEHVGMQSETLSQWQMSRVL"
     gene            463..1125
                     /locus_tag="PKI33_11900"
     CDS             463..1125
                     /locus_tag="PKI33_11900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNM37762.1"
                     /translation="MKLAALVPMRHHSQRVPGKNYRPLAGKPLFQHILETLQAVPEID
                     TVIVDTDSEPVMDGVRRLFPNVKLIQRPEHLRADNVPMNDILLYDTAQVQVDFYLQTH
                     STNPLLKTETISRGIKSFLADYPKYDSLFSVTRLQTRLYWQDGRAINHNPLELLQTQD
                     LPPVYEENSCVYLFTRENLERKKHRIGDKPFMFEIDADEAWDIDEELDFEIADFLMRK
                     RQ"
     gene            1129..>2109
                     /locus_tag="PKI33_11905"
     CDS             1129..>2109
                     /locus_tag="PKI33_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37763.1"
                     /translation="MQSLKQKLGTFGWGSLFLTLLGAVTYLPFAARMGYINDDWYLMF
                     DGYVGGASFFHEVFIVDRPLRGYVMQTAFSLFGMNPLYYHLSAFLFRVVSAVALFWIG
                     NHLWQRRTTYNWLAALLFLVYPGFLSQVNPIDYQSQIFSLACGMVSVALTLKALQAKT
                     SIAQWAYISVSIGLTWVYLGLVEYFLGFETLRFAVIGILYLRQTEQPVFIRLKSAVVT
                     SLPFLAGVGGFLVWRLFFFEAERKATDVSLQLGSAFTSPLTVLWWLNYLVQDVFKVIM
                     VAWTVPLNLFGFSLRLRDAFMGLGLAAIAVALLLFFLRQSNEKNGDDESDQ"
BASE COUNT          485 a          518 c          504 g          602 t
ORIGIN      
        1 tatgaaagag taggctttac taaaatccgc aggctggtgg gctataaact cgaaaacccg
       61 caagttgaat ccgatgtaga actcgaagaa attgacattc gtgaattggc gcgtttggtc
      121 gcttatcatg gactcaaaga ccttccctgg caactttcgg gtgctactgt catgcaacac
      181 acaccgcctt cacgcgcatt tcacgtcaac gatgcctatt gtcttatcac caaccccgaa
      241 gcgaccgaca tcgccatttc gtcggtgttg gtcaaaaccc gctcacgtgg agcaggcttg
      301 agcgcggttc tgttgcgcgc actttttgcg aagttcccaa ataaagtttg gcacgtctcg
      361 ccgatctatc ctgaagaaat gggcgttatc ttcgaacatg tgggcatgca gagtgagacg
      421 ctttcacaat ggcaaatgtc acgcgtgttg taaaataagg gcatgaaatt agccgccctt
      481 gttcccatgc gtcatcatag ccagcgtgtg cctggcaaaa attatcgccc gctcgcaggc
      541 aaacccctct ttcaacatat tttagaaacc ctgcaagccg tgccagagat tgacacggtt
      601 attgtggata ccgactcaga gccagtgatg gatggagtgc gccgcctctt tcctaacgta
      661 aaactgattc aacgccccga gcatctccgt gctgacaatg ttcccatgaa cgatattctc
      721 ctgtacgata cagcgcaagt acaggtagat ttttacttgc aaacccattc caccaatccg
      781 ctgctcaaaa ccgaaaccat ctcccgtgga atcaagtcct tccttgctga ttaccctaaa
      841 tatgactcgc tcttctcggt gacgcgctta caaacccgtt tatattggca ggatgggcgc
      901 gctatcaatc acaatccact ggagctattg caaacccaag accttccgcc cgtgtatgaa
      961 gagaattcct gcgtctattt gttcactcgt gagaatctgg aacgcaaaaa acatcgcatt
     1021 ggcgataaac cttttatgtt cgagatcgac gccgacgaag cctgggacat tgacgaagaa
     1081 ttggattttg agatcgcaga ctttttgatg aggaagagac agtaattcat gcaaagcctg
     1141 aaacaaaaac ttggcacatt cggttggggc agcctttttc tgactctcct cggtgcagtg
     1201 acttatcttc cctttgccgc acgcatgggg tatatcaacg atgactggta tctcatgttt
     1261 gacggttatg tgggtggagc ctcttttttc catgaagtat tcatcgtaga ccgtcccttg
     1321 cgcggatatg tgatgcagac ggcgttctcg ctctttggca tgaatccgct gtattaccac
     1381 ttgagtgcat tcctgttccg tgtggttagt gctgtggctt tgttttggat cgggaaccac
     1441 ctttggcaac gtcgcaccac ctacaactgg ttggcggcat tgctgttctt ggtctatccg
     1501 ggttttcttt cgcaggtaaa ccccatcgac tatcaatcgc agatcttcag ccttgcttgt
     1561 ggcatggttt ctgtggcgct gacgttgaaa gcattgcaag cgaagacatc tatagcacag
     1621 tgggcataca tcagcgtttc tattgggctg acatgggttt accttggctt ggtggaatac
     1681 ttccttggct ttgaaacatt gcgttttgct gtcatcggaa ttttgtatct tcgccaaact
     1741 gagcaacctg tttttatccg tttgaaatct gcagttgtaa cctctctacc cttcctagca
     1801 ggtgtgggcg gatttctagt ttggcgtttg ttcttctttg aagcagaacg caaagcgacc
     1861 gatgtcagct tgcaattggg ctctgccttt acatctccgc tgacagtctt atggtggttg
     1921 aattatcttg ttcaagatgt gttcaaggtc atcatggtag cgtggacagt gccgctaaat
     1981 ctcttcggct tctctctgcg gttgcgtgat gcctttatgg ggcttgggtt ggcagccata
     2041 gcagttgctt tgctgctatt ctttttgcgt cagagtaatg aaaagaacgg ggacgatgaa
     2101 tcagaccaa
//
LOCUS       DAOREF010000601         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41656_length_2107_cov_3.987817, whole genome shotgun sequence.
ACCESSION   DAOREF010000601 DAOREF010000000
VERSION     DAOREF010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41656_length_2107_cov_3.987817"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKI33_11910"
     CDS             <1..538
                     /locus_tag="PKI33_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM37764.1"
                     /translation="LAEAQAQYRQNLQSSWRVFSREEETIGYHQGLMGGKKISKPVET
                     EEIREAINRGNVLVLNSETDKREATIVVPIKLRGQIIGTMNIKAPSANRTWSRNEVNL
                     AEAISERLSLALENARLIQESQRQVIKEQTISEVTGKIGASINLKSVLQTAVEELGHA
                     IPGSEVLIEFEQKNENME"
     gene            546..>2107
                     /locus_tag="PKI33_11915"
     CDS             546..>2107
                     /locus_tag="PKI33_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNM37765.1"
                     /translation="MTAAYSETPIFQNREQEQRERIALTVARAFLVTSTIISFSILYI
                     AISLNSWQLFTLYGGNILYILLTYAGLQQLQKKNTLFGIFLVTGSVHLTIILLPILFE
                     GIATLAGLFSTLAAALILSQTLETRVARGAIVVSNITGIGIILLGSIPFPVRIQIPSV
                     LVVTIAILAIIETAIFMVLIWRQFRTFNIQTKLTLVFITILVTSLLVTGGYNAVQSYR
                     TQRAQTANNLENELGVKRSVIIDFLEASRQDVVFLGEAEVVQSYVKTIDDIAAPNIVV
                     RARTIMEREFRRFAESRGIYQEIRFLNNDGNEVIRIDTALDGNSSIVPQAELRQKGEL
                     DPARELSYFAQTITLPAGRLFQSPINLRKLDGTIVTPHEPIIQFGVPLVINTQTKGVI
                     MANIYAEKFLGILSSSGDDTFLVDTNGYYIAHPDESKRWGNDLNNNITIKNDFPDLVN
                     NLNNGNSGSMESNGYLVIYTPITIPGETEPRWYIGSFVSIDSITKPIFESTYNALSLL
                     LLTIIASIFIMST"
BASE COUNT          631 a          500 c          440 g          536 t
ORIGIN      
        1 tcttgctgag gctcaggcgc agtatcgtca aaacctgcaa agcagttgga gagtgtttag
       61 tcgggaagaa gagaccattg ggtaccacca gggcttaatg ggtggcaaaa aaatatcaaa
      121 acccgttgag accgaagaaa tccgcgaagc aatcaaccgc ggaaacgtcc tcgtgcttaa
      181 ttcagaaaca gacaagcgag aagcaacgat cgttgtcccg atcaaactac gtggacagat
      241 tattggcaca atgaacatca aagcaccttc agcaaaccgc acctggtcca gaaatgaagt
      301 caatcttgcc gaggcaattt cagagcgtct ttccctagcc cttgaaaatg cccgcctgat
      361 tcaggaatcg caaagacagg taatcaagga acaaaccatc agtgaagtta ctggaaagat
      421 cggcgcatcc atcaacttaa agagtgtact tcaaacggca gtagaagagt taggccacgc
      481 tattccggga tccgaagtac tcattgaatt cgaacaaaag aacgagaata tggagtaatc
      541 ccctaatgac tgctgcttat tcagaaaccc ccatattcca aaacagggaa caagagcaac
      601 gtgagcgtat tgcgctgaca gttgcaagag cattccttgt caccagcacg atcatttcat
      661 tctccattct ttacattgcc atcagcctta acagttggca gctttttact ttgtatggtg
      721 gcaatatttt atatatcctt ttgacatatg ctggcttgca acagcttcaa aagaaaaaca
      781 ccttgtttgg gatttttcta gtaactggtt cagtccactt aacaatcata ctattgccga
      841 ttctattcga aggcatagcc accctggctg gtctttttag cacgttggca gcagcactaa
      901 ttttgagtca gacccttgag accagggttg cccgaggggc tatcgtagtc agcaatatca
      961 ctgggatcgg catcatcctg cttggatcca tcccgtttcc cgttagaatt caaatcccaa
     1021 gcgttttggt ggttacaata gccatccttg cgataattga aactgccatt tttatggtat
     1081 tgatctggcg tcaatttaga acatttaaca tccaaacaaa actgacgctt gtctttatta
     1141 caattcttgt cacatcattg ctggtcacag gtggttacaa tgccgtccaa agctacagaa
     1201 ctcagcgggc acaaaccgca aacaacctgg aaaacgaact tggtgtaaaa cgatcggtca
     1261 ttatcgattt cctcgaagct tccagacagg atgtagtctt cctcggagaa gcagaagttg
     1321 tacaaagtta tgtaaagacg atcgatgaca ttgctgcccc caacattgtt gtcagagcga
     1381 gaaccattat ggagcgtgaa ttccgccgtt ttgcagaaag ccgcgggatt tatcaagaaa
     1441 tacgcttcct gaacaacgac gggaacgaag taattcgaat agataccgct ctggatggta
     1501 attcatccat tgtgccgcag gcggaactac ggcagaaagg tgaacttgat cctgcaaggg
     1561 aattaagcta tttcgcgcag acaatcaccc tgccagctgg tcgtttattc cagtctccaa
     1621 tcaacctcag aaaactggat ggaacgatcg tcacaccaca tgagccgatc attcaatttg
     1681 gtgtacctct cgtcattaac acgcaaacca aaggcgtgat catggcaaat atctatgccg
     1741 agaaattcct cgggattctc tcctcatcag gtgatgatac ttttttggtg gacacaaacg
     1801 gttactacat tgcccatcct gatgaatcca agaggtgggg caacgatcta aacaacaata
     1861 taaccatcaa aaatgacttt ccagacctcg taaacaatct taacaatgga aactctggaa
     1921 gtatggagtc aaatggatat cttgtaatct atacccccat cacgatccct ggcgaaacag
     1981 aaccacgctg gtatattggc tcatttgtct ccatagactc gatcacaaaa ccgatcttcg
     2041 aatcaaccta caatgcgtta tctttgttat tgctaacgat catcgcctct atttttatta
     2101 tgagcac
//
LOCUS       DAOREF010000602         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41896_length_2100_cov_3.750611, whole genome shotgun sequence.
ACCESSION   DAOREF010000602 DAOREF010000000
VERSION     DAOREF010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41896_length_2100_cov_3.750611"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1419)
                     /locus_tag="PKI33_11920"
     CDS             complement(106..1419)
                     /locus_tag="PKI33_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNM37766.1"
                     /translation="METNLVLEQPVYKTSTFEFLWNVFRNPLHALTDLAHEQGDIARV
                     KLRKRDLFLLSHPAFIEQVLVRQADNFVKGPSLQRARIILGEGLLTSEGDEHLTQRRT
                     LQPAFHRHKMEEYIPLMNENTMAHIANWQDGERADMSEGMMRLTLNIALWSFFGNAPS
                     GTVDKVSDAMKTLVRLFPLAQMPLPESARSLFPSFKEAKADLSEVTEALLKNPNSEYA
                     KRALVNILKENHKDQFTDEQVHAHALTFLLAGHETTALLLAWCWDLLAHHPMAQAILQ
                     AEVDSVLGDRLPTPEDIQKLPYTRAVVKETLRMRPPAWAMGREALHDCEIGGQFIPAG
                     STVIMSQWGTHHDSRFYYEPLHFRPERWFDLEGTSTPRYSFFPFGGGNRVCIGEHFAM
                     TEAITILAVVARRWNISPAQRELATPKPSVTLRPEKNLNVIVTKR"
     gene            complement(1563..2024)
                     /locus_tag="PKI33_11925"
     CDS             complement(1563..2024)
                     /locus_tag="PKI33_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37767.1"
                     /translation="MTGVDVLKQIRPEYPDLPILILTSFTDDAHVFAALRAGASGFLL
                     KEMNGDDLVAAIRGAAQGKPQLHPDIAKRLMARAPMPDDPFDSLTERERGILKLLAKG
                     KSNKEIAGELSLTEMTVKGYVSDMFVKLGVNDRTQAALMAVRYGLVLPEEL"
BASE COUNT          511 a          555 c          526 g          508 t
ORIGIN      
        1 ggcggtgtca gcgggtaacc cacaggcagt gataagagcc gtgagtatca aggtcaatac
       61 aagggttcga aggaaagatt tcatgttata tctccaattt tgtttctatc gtttcgtcac
      121 gatcacattc agattttttt cagggcgcag tgtcacactt ggtttcgggg tggcaagttc
      181 acgttgagcg ggtgaaatat tccaacgcct tgcaacgaca gccagaatgg taatggcttc
      241 ggtcatagcg aaatgctcac caatgcagac gcgattgccg ccgccgaagg ggaagaaact
      301 gtaacggggt gttgaagttc cttcgaggtc gaaccagcgt tcggggcgga agtgaagcgg
      361 ttcgtaataa aagcgtgagt cgtgatgggt cccccactgg ctcataatca cggtcgaacc
      421 cgccgggatg aactgcccgc caatctcgca atcatgcagg gcttcgcgtc ccatcgccca
      481 agccggtggg cgcattcgca aggtctcttt caccactgct cgtgtgtatg gaagtttttg
      541 aatatcttcg ggcgtgggca aacggtcacc taaaaccgag tcgacttccg cttgcaaaat
      601 agcttgcgcc attgggtgat gcgcaagcag gtcccaacac caggcgagta gcaaggctgt
      661 ggtttcatgc ccagccagca agaacgtgag cgcatgcgcg tggacctgct catcggtaaa
      721 ttggtcttta tgattctctt tgaggatatt gaccaaagcc cgcttggcat attcagagtt
      781 tggattcttg agcaacgcct ctgtgacttc actcaggtct gctttggctt ccttgaagct
      841 tgggaataag ctgcgagcgg actctggcaa aggcatttgc gccagcggaa agagacgaac
      901 caaggtcttc atcgcatcgc tgactttatc cacagttcct gatggcgcat tgccaaagaa
      961 tgaccacaac gcgatattaa gcgttaatcg catcatacct tcagacatat ctgcccgctc
     1021 gccatcttgc cagttcgcaa tatgtgccat cgtattctcg ttcatcaagg gaatgtattc
     1081 ttccatcttg tgacgatgaa acgcgggttg caaggtgcga cgttgagtaa ggtgttcatc
     1141 cccttcgctg gtgagtaacc cttcgcctaa aatgatgcgt gccctctgca agctgggacc
     1201 tttcacaaag ttatctgcct gccgcaccaa gacctgttca ataaatgccg ggtggctcag
     1261 tagaaagagg tcacgcttgc gcaatttgac tcgtgcaata tcaccctgtt catgcgcgag
     1321 atcggtcaac gcatgcaacg ggttgcgaaa gacattccat aaaaattcaa aggtggatgt
     1381 tttgtaaacg ggttgttcaa gaactaaatt tgtttccatc ggctatctcc ttcaagtgat
     1441 aaccaaagga tacttttttg aaccgattaa gtccgcccgc gaaggtgggg aacctctccc
     1501 cctatttggt gggtatataa aaaaagtgac agtcacctta aaggtgactg tcactttgag
     1561 ttctacaatt cttccggcaa aaccaaccca tagcggaccg ccatcagcgc cgcctgagtt
     1621 ctatcgttca cccccaactt cacgaacatg tcactgacat agcccttcac cgtcatctcg
     1681 gtcagcgata actcgccagc aatttcttta ttgctcttac cctttgccag caatttcaaa
     1741 atgccgcgct cacgttcggt caacgaatca aacgggtcat cgggcatggg agcgcgcgcc
     1801 atcaggcgct tcgcaatatc agggtgcaat tgcggtttgc cttgcgccgc tccacggatc
     1861 gctgccacca aatcatcgcc attcatttcc ttcagcaaaa atccgctcgc acccgcacgc
     1921 aacgccgcaa agacatgcgc atcatcagtg aacgaagtca gaatcagaat cggcaaatca
     1981 gggtattccg ggcggatctg cttcaacaca tccacaccgg tcatcttggg catttgcatg
     2041 agggacagaa tgccttcctg catacgggcg ccgccggagg ccgcgaacag gatgaacgga
//
LOCUS       DAOREF010000603         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_41899_length_2100_cov_3.497800, whole genome shotgun sequence.
ACCESSION   DAOREF010000603 DAOREF010000000
VERSION     DAOREF010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41899_length_2100_cov_3.497800"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKI33_11930"
     CDS             complement(<1..543)
                     /locus_tag="PKI33_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37768.1"
                     /translation="MQPQLRKKRVLIADTTQETRRNTRVMLSTVKGVEVVAIALDGNQ
                     AVEMAKLHKPDIVLLDINMSKLNGLNAYKQILKVNPTAACILVSAENHPETVEAAKEM
                     GIQNFLVKPFTEHELEHAMQDVIQRLDGKLNNQRTIEVRKLADEYVRTKRTDEEAVQV
                     FEEAIQLPDSELVWMQTLAMI"
     gene            complement(637..1659)
                     /locus_tag="PKI33_11935"
     CDS             complement(637..1659)
                     /locus_tag="PKI33_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNM37769.1"
                     /translation="MQMKKWQFWLGVLISILFLWLSLRGLHLGDFWGTMLKANYIWLV
                     PGILVYFIGVWVRAWRWHYLLGPIKKIPTKTMFPITTIGYMGNNIYPARAGEVLRAVI
                     LKRKEDVPVSASLATIIVERIFDGVVMLAFVFVNLSELAKLTGASGFVGNIQQVAVIG
                     TGVFLGALLVFLLAAMFPQFTSRVGSWTIERLTPQRLHEKLTGIMNKFLDGLASLRSP
                     FNVLMVFFTSVVIWLLETAKYWFVMHAFNFTVSFFALMLMNGIVNLATTIPSAPGYIG
                     TFDAPGIAVLTAYGVDQATAAGYTLVLHVALWVPITALGAYYLAREGIKWSDTLREEA
                     ETPNTP"
     gene            1747..>2100
                     /locus_tag="PKI33_11940"
     CDS             1747..>2100
                     /locus_tag="PKI33_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNM37770.1"
                     /translation="MKKYFPTIRDYLLIVASSLIQAVSLRLFFIPANLASGGVSGISQ
                     LINHFTGWPIGLMVLIGNIPLFLLGWRFLGGFKFAMRTAVAIVTYSIFTDLVMKIPAF
                     VSYSQILINDLQGDIF"
BASE COUNT          514 a          528 c          515 g          543 t
ORIGIN      
        1 gatcatagca agtgtttgca tccaaaccaa ttcactgtca ggaagttgaa tggcttcttc
       61 aaaaacttgc acagcttctt cgtccgttcg tttggtgcgg acgtattcat ctgccaactt
      121 tctgacttcg atagtgcgtt gattgttaag cttgccatcc agccgttgga taacatcttg
      181 catggcatgt tccagttcat gttccgtaaa cggtttgacg aggaaattct gaatgcccat
      241 ttccttggcg gcttctacag tttcaggatg attttcagcc gagacgagga tgcacgccgc
      301 agttgggttg acctttagaa tctgtttgta agcattcaac ccattgagtt tagacatgtt
      361 aatgtcgagc aatacgatat ctggtttgtg tagctttgcc atttcaacgg cttgatttcc
      421 gtcaagtgca atggcaacca cctcaacacc tttgacagta gaaagcatta cacgcgtgtt
      481 acggcgggtt tcttgtgtag tatctgctat aaggactcgt ttttttctaa gttggggctg
      541 catatcgtcc tgccttctta atagggcaat tggttcaacc tattaagtat aaccgaggcg
      601 ggcatccgaa gatgcccgcc tgtcagagct gtaaatttat ggggtgttgg gtgtttctgc
      661 ttcctcgcga agcgtgtcgc tccacttgat gccctcgcga gccaggtaat aggcacccaa
      721 tgcggtgatg ggaacccaca acgccacatg caacacgagg gtatagcctg ccgctgtggc
      781 ttggtccacg ccgtaggcag tgaggacggc gatgccgggc gcatcgaaag tgccgatata
      841 gcccggtgcc gaaggaatgg tggtggcgag gttgacgatc ccgttcatca gcatcagtgc
      901 aaaaaatgag actgtgaagt taaaggcgtg catcacgaac caatacttgg cggtttccaa
      961 aagccaaatg accaccgaag tgaagaagac catcaacaca ttgaagggcg aacgaagcga
     1021 agcaagccca tccagaaatt tattcatgat accggtcagc ttttcgtgca aacgctgcgg
     1081 ggtcaggcgc tcaattgtcc acgaaccgac tcggctggtg aactgtggga acatggctgc
     1141 cagcaaaaag accagtaatg cgccgaggaa gaccccggtt ccaatcaccg ccacttgttg
     1201 gatgttcccc acaaagccag atgcccctgt gagttttgcc aactcagaca agttcacgaa
     1261 cacgaacgcc aacatcacta caccgtcgaa gatgcgctcc acgatgatgg tcgccagcga
     1321 agcagagacg gggacatctt ctttgcgctt caagatgaca gcgcgcagca cctcgcccgc
     1381 gcgcgcgggg tagatgttat tgcccatata cccaatggtc gtgatgggaa acatggtctt
     1441 ggtgggaatc tttttgatgg gacccagtaa ataatgccag cgccatgccc gcacccatac
     1501 cccaatgaaa tacaccagaa tacccggcac cagccagata tagttcgcct tcagcatcgt
     1561 gccccaaaaa tcgccaagat gcagcccgcg cagggaaagc cataaaaata ggatgctgat
     1621 cagcaccccg agccaaaatt gccatttctt catttgcata attgttgatt gtaccaaaat
     1681 gaaaggtgct aaacgagacc gccctctttg ccctcgcgta taattccagc cttgaaagga
     1741 acgtgcatga aaaaatattt tcccactata agagactatc ttcttattgt tgcatccagt
     1801 ttgattcagg cggtaagttt gcgcctgttc tttatccctg ccaatctagc ttcaggtggg
     1861 gtgagcggta tttcgcagtt gattaaccat ttcactggct ggcccatcgg tttgatggtg
     1921 ctgattggca atattccgct ctttctttta ggctggcgct tccttggcgg attcaagttc
     1981 gccatgcgca ccgccgttgc cattgtgacg tattcgatct ttacagacct ggtgatgaag
     2041 attcctgctt ttgtctctta ttctcaaatt ctgatcaacg acctgcaagg cgatattttc
//
LOCUS       DAOREF010000604         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42180_length_2091_cov_4.237721, whole genome shotgun sequence.
ACCESSION   DAOREF010000604 DAOREF010000000
VERSION     DAOREF010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42180_length_2091_cov_4.237721"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PKI33_11945"
     CDS             <1..1199
                     /locus_tag="PKI33_11945"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HNM37771.1"
                     /translation="WLGILSILATLFAIIVSLFPSDNPSLIVVVFKFLLIMTPILSSG
                     LAVYGSKFLSSGDWLVTRAGAEEILKEIYTYRTVLKNSRTRDAWLENRLAEIQRSVFR
                     GLNGELTIPPFEGYLPPHYNQSDPYSDPGFSDLIGDEYFQYRLESQLGWHVRKVNKFQ
                     RERVRLQIFIILSGVFGSILAVILPVWTALAASFTAVFLGWQELRNLDSVVRNYSKVV
                     MELTLLYDHWKNLKPGQKTPSEFYKMVRSTEDILWSQNVEYIKAMQEALKESDLEEEA
                     SLVNRVIKEARESDRRLKQSMEDAVVEMTTEKLHEAEEKLSETFEEALNTLAEEASSE
                     IVQAELAAMREAIQEFAENAWQGLSTSLKEIADEYKDIDVGTDTPRDVLNSLLARYPK
                     TSEVKG"
     gene            1228..>2091
                     /locus_tag="PKI33_11950"
     CDS             1228..>2091
                     /locus_tag="PKI33_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37772.1"
                     /translation="MTGSTETAQKADTTNTPATPAQAQPAAPGTENKGVPIIGPAGEP
                     AKNVDDSTVAYKEVDKSSLVVEVRPHAFIVMPFGKKKGGDGSLYDFNAIYKQLIKPAL
                     EEAGFEPFRADEETASGDILTDMFQELLLADLVLCDLSIDNANAFYELGIRHAFRKRG
                     VMHIQAGRAYMPFDIFNVRTLPYHVTPDGVPDAAFLKTDIQAIARMAKDTWASDRDAI
                     HSPIFNLLQGLKEPERKSLRTPLATGFWREYNEWKQRVTVAPRQKRIGDILLLTEEIS
                     NPLIKEEAIGEA"
BASE COUNT          568 a          538 c          473 g          512 t
ORIGIN      
        1 gttggcttgg cattctcagt attctggcga ctttgttcgc gatcattgtg tcgctattcc
       61 catctgataa tccatcgctg attgttgtcg tcttcaaatt cctgttgatt atgaccccca
      121 tcctttcttc tgggttagcg gtctatggaa gcaaattcct ctctagtgga gattggttgg
      181 tcacacgtgc tggtgctgaa gaaattctca aagaaatcta tacctatcgc accgttctaa
      241 aaaattcacg cactcgcgat gcctggttgg aaaatcgctt ggctgaaatt caacgttcgg
      301 tctttcgcgg tttgaacggc gaattgacca tcccgccctt tgaaggctat ttaccgcctc
      361 actacaacca atcagacccc tatagcgacc ccggttttag cgacctaata ggcgatgaat
      421 attttcaata tcgccttgaa tctcagcttg gttggcatgt cagaaaagtc aacaagttcc
      481 agagagaacg tgtgcgctta caaatcttta tcatcctctc cggtgttttc ggctccattt
      541 tggctgtcat tctcccagtt tggacggcac tcgccgcttc gttcacggct gttttcctcg
      601 gctggcagga acttcgtaat ctagactcag ttgtccgcaa ttacagcaag gttgtcatgg
      661 agttaactct cctctacgat cattggaaga acctcaaacc cggtcaaaag actccttcag
      721 aattctacaa gatggttcgt tctacagaag atatcctctg gagccagaac gtggaataca
      781 ttaaagccat gcaagaggcg ctcaaagagt ctgacctcga agaagaagcc agccttgtca
      841 accgcgttat caaagaagca agagaatccg atcgccgctt gaaacaatcc atggaagatg
      901 cagtggtaga aatgaccact gaaaagctac atgaagctga agaaaagtta agcgaaacct
      961 tcgaagaagc tttgaataca ttggcagaag aagcctcttc tgagatcgtt caagcagagc
     1021 ttgctgcaat gcgcgaagct attcaagaat ttgctgaaaa cgcatggcag ggactttcta
     1081 cctccttgaa agaaattgca gacgagtaca aggatatcga tgtcggtacc gatactccgc
     1141 gtgatgttct caacagcttg cttgcccgtt acccgaagac cagcgaagtc aagggataga
     1201 gtcatatccg cttcatagga gattacaatg accggctcaa cagaaactgc acagaaagct
     1261 gatacgacca atacaccagc aaccccggca caagcacaac ctgccgcgcc tggtactgaa
     1321 aataaaggtg tccccattat tggtcccgct ggagaacctg cgaaaaacgt agacgactct
     1381 accgtggcat ataaagaagt ggacaaatcc agcctcgttg ttgaagtgcg tccgcatgca
     1441 ttcattgtta tgccttttgg aaagaaaaaa ggcggcgacg gctctcttta tgacttcaac
     1501 gccatttaca agcaactgat taagcctgcg ttggaagaag ccggcttcga accttttaga
     1561 gcggatgaag aaaccgcctc gggcgacatc ctcaccgata tgttccaaga gttgctgctt
     1621 gccgatctag tgctatgtga cttaagtatt gataatgcca atgcttttta tgaactaggc
     1681 atccgccatg cgttccgcaa acgcggcgtg atgcacattc aagctggtcg cgcctacatg
     1741 ccgttcgata tcttcaatgt gcgcaccctt ccctatcatg tcacccccga tggcgtgccc
     1801 gacgccgcat tcctcaagac agacattcaa gccattgccc gtatggcaaa agacacctgg
     1861 gcttctgacc gagatgcaat tcacagccca attttcaact tgctccaagg gctgaaagaa
     1921 ccggaacgaa agtcgcttcg cactccgctg gcaactggat tctggcgcga atacaacgaa
     1981 tggaaacaac gtgtaaccgt cgcaccgcgc caaaaacgca tcggcgacat ccttttactc
     2041 acagaagaga tcagcaaccc actaatcaag gaagaggcga ttggtgaagc t
//
LOCUS       DAOREF010000605         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42244_length_2089_cov_3.648968, whole genome shotgun sequence.
ACCESSION   DAOREF010000605 DAOREF010000000
VERSION     DAOREF010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42244_length_2089_cov_3.648968"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1461)
                     /locus_tag="PKI33_11955"
     CDS             complement(145..1461)
                     /locus_tag="PKI33_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37773.1"
                     /translation="MNILHKKTPLNFYTLIIVIITSSCSQPKETLSPISTSQPNSKTT
                     ITIIPSPTITSEPINPGGTIIYSDYKTVYLVDLQSLKTKSVFSTDGFLRTFISKDYIY
                     INNRTSLESSDAKLETFRMSVEGAYLEQFEIADETKFPFSERINISPNERYIAYYKNG
                     KIVIVDTSTKTTQSIYAPPPHGFLSFAWASDSNRIFIVDMIFHNAIGKHMRILQYSLD
                     SQKTTELLSDFPDPEFKWFIEDSLWYWSPDSKYLLIKNHAGVHLFDTEKKSLKKISIN
                     HESTANFTWSTNGKHLLVHERKGSEQIAFYIFDIENSEAKDISDFAPIQYGFTCLWSP
                     NGSMILCESVDKHLYLIDLIKKVSRDIAWVDGLKYSDRSGTFLLWFFHPAWSSDSKYF
                     SYLTMSNPSSTAYEQDFILNIQSVETGAYLQIQIPREKEIEQIYWR"
     gene            complement(1643..>2089)
                     /locus_tag="PKI33_11960"
     CDS             complement(1643..>2089)
                     /locus_tag="PKI33_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNM37774.1"
                     /translation="THITIKYRFPIENVDEVEKVVQEFSLAQRKTKWLLQGFSHFKND
                     DDFVIFIDVIAPEETRKAHADFLDRLRKIGWVKWGKFDNANLHYHVTLVAQSLTPENF
                     EAVWPFVNEQEKPNFESFFDNLTLVQINEDSRSVYKTYWLQNRKAG"
BASE COUNT          622 a          375 c          408 g          684 t
ORIGIN      
        1 gacggcgaag ccgtccagcc agaaaaaggc taaggcgtag gaaactgctt gggattgccg
       61 cagagtcccc agcgtcaggt gcacgctttg ttagcccgca ctctttctgg aaagcgttta
      121 aattttacac tagttacaaa aacattatct ccaatagatc tgttctatct ctttttctct
      181 tggaatttga atttgcaagt acgcgccagt ttcaacactt tggatgttga gaataaaatc
      241 ttgctcataa gcggttgagg aaggatttga cattgttaag taagaaaaat atttactatc
      301 ggaagaccat gcaggatgaa aaaaccataa aagaaatgtt ccactgcggt ctgaatactt
      361 aagtccatca acccatgcaa tatctcttga tactttttta attaaatcaa ttagatataa
      421 atgtttgtca acagattcgc ataaaatcat gcttccattt ggagaccata aacaagtaaa
      481 cccatattgg attggcgcaa aatctgagat gtctttcgcc tcgctgtttt caatgtcaaa
      541 aatataaaat gctatttgct ctgaaccttt tctttcatgg accaaaagat gtttcccatt
      601 tgtagaccat gtgaaatttg ctgtagattc atgatttata ctaatttttt tcaaactttt
      661 tttctcggta tcgaacaaat gtacacccgc atgatttttt atcagtaaat atttgctgtc
      721 tggtgaccaa taccaaaggc tatcttcaat aaaccacttg aattccggat cagggaagtc
      781 agatagtaat tctgttgttt tctggctatc taaagagtat tgtagtattc gcatatgttt
      841 gccaattgca ttatgaaata tcatgtcaac tataaaaatc ctgttactgt ctgatgccca
      901 agcgaaagag agaaatccgt gaggaggagg cgcgtaaatg ctttgggtag ttttggttga
      961 ggtatcaaca ataacgattt ttccgttttt gtaatatgca atgtatcgct catttgggga
     1021 aatatttatt ctctcgctga acggaaattt agtctcgtct gcaatttcga attgctccaa
     1081 atatgcacct tctacactca tcctgaacgt ttcaagcttg gcgtcactgc tttcaagtga
     1141 tgttcgattg ttgatgtaga tataatcttt tgaaatgaag gttcgtaaaa atccatcagt
     1201 tgaaaatact gatttggttt ttaggctttg aaggtcaact aaatagactg ttttgtaatc
     1261 cgaataaatt atagttccac ctggattaat cggttctgat gtaattgtag gactgggaat
     1321 tatggtaatt gtcgtctttg agtttggttg tgaagttgat attggagaaa gcgtttcttt
     1381 gggttgactg caactggaag taatgataac aataatcaat gtatagaaat tgaggggcgt
     1441 ttttttatgc aatatattca tgagtttcca cctatacaga ataagacagc gtttaagacc
     1501 tctgattatg aagagacggg ctaacggttt gcgttacctg cgctggggcg gggacggcga
     1561 agccgtccaa ccagaaaaat gataaggcgt aggaaactgc ttgagatttg cgcagaatcc
     1621 ccagcgtcag gtgcacgctt tgttagcctg cttttctgtt ttgaagccaa tacgttttat
     1681 aaacagaacg tgaatcttca ttgatttgta ctaacgtgag attatcaaaa aaagattcaa
     1741 aattcggttt ttcttgctca ttcacaaatg gccaaactgc ttcaaagttt tcaggagtta
     1801 gactttgagc aactaaagtc acatggtaat gcaaattggc attgtcgaac tttccccatt
     1861 ttacccaacc tattttccgc agtctatcca gaaaatcagc atgagcttta cgggtttctt
     1921 cgggtgcaat aacatcaata aaaatcacaa aatcatcatc gtttttgaaa tggctaaaac
     1981 cttgtaaaag ccattttgtt tttctttgag ccagactaaa ctcttgaacc actttttcaa
     2041 cttcatcaac attttcgatt ggaaaacgat attttatggt gatatgagt
//
LOCUS       DAOREF010000606         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42536_length_2082_cov_3.734583, whole genome shotgun sequence.
ACCESSION   DAOREF010000606 DAOREF010000000
VERSION     DAOREF010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42536_length_2082_cov_3.734583"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKI33_11965"
     CDS             <1..246
                     /locus_tag="PKI33_11965"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNM37775.1"
                     /translation="IVVESNPSTGYHWEIVGDLTNIEFVSREFTGSEPPIPGSGGVEV
                     WAFKATTAGDTSFVLGNYPPGEGTTYEQQVQFSVTVQ"
     gene            complement(293..706)
                     /locus_tag="PKI33_11970"
     CDS             complement(293..706)
                     /locus_tag="PKI33_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37776.1"
                     /translation="MKITRDVINDLLPVYQSGEASEDTQRLVEEFLQTDPEFAELVAV
                     QDQPLEKLNINISKETEMKTLQDTRALLQKRSIYLAFTIFFFLLPASFTFDSNGIYWM
                     WAKAPINVLIFVAFGIFNAVQYWRTSQKLKGSGLE"
     gene            complement(703..1197)
                     /locus_tag="PKI33_11975"
     CDS             complement(703..1197)
                     /locus_tag="PKI33_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNM37777.1"
                     /translation="MVTNFHELYQTYAEDVYRFAYWLCGDAQDAEDITSETFVRALTA
                     SGEIRAETVKGYLLTIARNLAYKRSQREKRFVPLSADLVERQAEPSQLAEQAMNLRDA
                     VQFIQTLPETDRTALVLRLQNELTYEEIASILNISLAAAKVKVHRARMKLTEYMRHQE
                     ELSQ"
     gene            1508..>2082
                     /locus_tag="PKI33_11980"
     CDS             1508..>2082
                     /locus_tag="PKI33_11980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM37778.1"
                     /translation="MFHVLLWRIRFLFTSKYASHLAKYSSDLGSAKLLEVGCGSARTL
                     HYLAEHYQKSQCYALDLSPQAVNVAKKLSPSFHAGVASAFDLPIPENNVDISFSIGLI
                     EHFSREQAAQMVREKIRVTRARGIVGIVVPWQSSVYNLIVRKAFGKYWPFGNEYPFHR
                     QELADFMQELGLSDIKIHVVYGSAILGIGRK"
BASE COUNT          568 a          474 c          438 g          602 t
ORIGIN      
        1 atcgtcgtcg aaagcaaccc gtccaccggt tatcactggg aaatcgtagg cgacttgacg
       61 aatattgaat tcgtttcgcg tgaattcacc gggtctgaac ctcccatccc cggctctggt
      121 ggcgtagaag tctgggcatt caaagctact actgctggag acacctcatt tgtgcttgga
      181 aattaccctc ccggggaagg cacaacatac gaacagcaag tacagttttc cgtcacagta
      241 caataaataa aaaaacaccc cctgtttcta ggaggtgttt ttttactact gcttattcca
      301 atccgcttcc tttgagcttc tgcgaagttc gccagtattg aaccgcatta aagattccga
      361 aagcgacaaa gatcaaaacg ttgatcggag cctttgccca catccagtaa atgccgttcg
      421 agtcaaatgt aaatgaagct ggcagcaaga agaaaaagat agtgaacgcc aggtagatcg
      481 agcgtttttg taatagtgca cgagtatctt gcagtgtttt catttcagtc tcctttgata
      541 tattgatatt aagtttctca agcggctggt cttgcaccgc aacgagttct gcaaattcag
      601 ggtcggtttg aaggaattct tccacaagcc tctgtgtatc ttcgctggct tctcctgact
      661 gataaacagg taacaggtca ttgatgacat cgcgtgtgat tttcattgtg ataactcctc
      721 ttggtggcgc atatattcgg tcaacttcat ccgcgcccga tggactttga ccttcgcagc
      781 cgccagtgaa atattcaata tgctggcgat ctcttcatac gttaattcat tttgcaaccg
      841 taataccagt gctgtgcggt ctgtttctgg taaggtttgg ataaattgca ccgcatctcg
      901 caaattcata gcttgctcag ccaattgcga cggttccgcc tgccgttcga ccaagtccgc
      961 gctcaaggga acaaatcgct tctcacgctg tgaccttttg tacgccaaat ttcgagcaat
     1021 cgtcaacaga tagcctttca cagtctctgc cctaatctct ccgctggcag tgagcgcgcg
     1081 aacaaaggtc tcagaggtaa tgtcttctgc gtcctgagca tccccgcaaa gccagtaagc
     1141 gaagcgatac acgtcttctg cataggtttg gtagagttcg tggaaattag tgaccatacg
     1201 tctacagata ttatcccttc aaccctaaaa ggttacagtc aacgactatt aaattagaga
     1261 ccgacaatca tggttttata ttgattttga cccgctttta taacctaagt agagcctgtt
     1321 caattaatat agaacaaata ttcaaacaca ctatcacaaa tttgaaagga ttcaatctac
     1381 gatagagaat gacctctctg ataaaatatt ggttattaat taaattccac acgattggat
     1441 aagaaaattg tcacaaccta acggactttg ggactcattc tgggcaactc atgcaaccaa
     1501 ggataatttg tttcatgttt tgctttggcg aattcgtttt ttatttacca gtaagtacgc
     1561 ttcgcatctc gcaaaataca gttcagacct tggttctgca aaactacttg aagtaggctg
     1621 cggctctgca agaaccttac actacttggc tgagcattat caaaagagcc aatgctatgc
     1681 tctagatcta tcgcctcaag cagtgaatgt agccaaaaaa cttagccctt cattccatgc
     1741 aggcgttgca agtgcatttg acctacccat ccctgagaac aatgtagata taagttttag
     1801 cattgggtta attgagcatt tttcaagaga acaagctgct caaatggttc gggaaaaaat
     1861 tcgtgtcact cgcgctaggg gcattgtagg gattgttgtt ccctggcaaa gttctgtcta
     1921 taatctaatt gtccgtaaag cttttggcaa atactggccc tttggtaatg agtatccttt
     1981 tcaccgtcag gaacttgcgg attttatgca agaacttggt ttatctgata taaaaataca
     2041 tgtagtctat gggagtgcca tcctcgggat tgggcgcaaa ca
//
LOCUS       DAOREF010000607         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42619_length_2080_cov_3.829630, whole genome shotgun sequence.
ACCESSION   DAOREF010000607 DAOREF010000000
VERSION     DAOREF010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42619_length_2080_cov_3.829630"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="panC"
                     /locus_tag="PKI33_11985"
     CDS             complement(<1..500)
                     /gene="panC"
                     /locus_tag="PKI33_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNM37779.1"
                     /translation="MKIVSSLSDLRAARLSLDGTVGLVPTMGYLHEGHLSLVSRAKAE
                     NQHAIVTIFVNPTQFGPNEDLSKYPRDLERDLELLRPLGVDVVWTPTAEVMYPPGYQT
                     WVEVEALTSPLEGAMRPGHFRGVTTVVAKLFNATQPTKAYFGQKDAQQAAVIRRMVID
                     LNFPLE"
     gene            602..823
                     /locus_tag="PKI33_11990"
     CDS             602..823
                     /locus_tag="PKI33_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37780.1"
                     /translation="MAFGYTNSKGTTYYLHARGKMFFFAKEVKEGALDAVPAGYNVVE
                     MKTGMPVLKKAVAEGSATTTETTNTESAG"
     gene            955..1353
                     /locus_tag="PKI33_11995"
     CDS             955..1353
                     /locus_tag="PKI33_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNM37781.1"
                     /translation="MPALNRVQLIGRLGKDPESKYTPTGKKVCHFSLAVSNRWKDKNG
                     ETKETTEWVNIETWGRLGEVCQEYLKKGSLIFVEGRLKTDKYEDRESGETRYYTKIIA
                     QSLQFLDRKASEEPVMAVEEESGEYELAIE"
     gene            1453..>2080
                     /locus_tag="PKI33_12000"
     CDS             1453..>2080
                     /locus_tag="PKI33_12000"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM37782.1"
                     /translation="MPLPEKIGRYEVKSELGRGGMATVYRAVDPNSGREVAVKVLPRE
                     MMHDPQFRERFEREIKMIASLEHPAIVPVYDVGDIDGQPYFVMRFMTGGSLSDLIEKG
                     SIPIDETAKIIEKIAQGLAYAHRKGVIHRDLKPDNILFNDDGEPFISDFGVAKLSESS
                     SNLTGSGVIGTPAYMSPEQAQGNEIDQRSDVYGLGVIVYQMLSGHQPYS"
BASE COUNT          582 a          512 c          518 g          468 t
ORIGIN      
        1 atttccagcg ggaagttcaa atcgataacc atgcgtcgaa ttactgctgc ctgttgcgca
       61 tccttttgac cgaagtatgc cttcgtcggc tgggtggcat tgaacagctt cgccaccacc
      121 gtcgtcaccc cacggaagtg ccctgggcgc atcgccccct caagcggact cgtcaacgct
      181 tccacctcta cccaggtctg gtagccaggc ggatacatta cctccgccgt gggcgtccaa
      241 accacgtcca caccgagggg gcgtagaagt tctaagtcac gttcgagatc acgcggatat
      301 ttcgagaggt cttcgttcgg tccgaactgg gtagggttca caaaaatagt gacgatggca
      361 tgctggttct cggctttggc acgagagaca agcgataaat gcccctcgtg aagatacccc
      421 atcgtgggaa caagaccaac cgtcccgtcg agagagaggc gagcagctct taggtcagag
      481 agagaggaga caattttcaa gtcaggctcc aatttgtcta tacatttaca aaagtactac
      541 ttgacaggct agaaattttg ttctacactt ttatcagaat ttagacaaaa ggagaaatac
      601 aatggctttc ggatacacca actcaaaagg aactacctac taccttcacg ctcgcggcaa
      661 gatgttcttc ttcgctaaag aggttaagga aggcgctctt gatgccgtac ccgctggtta
      721 caatgtggtc gaaatgaaga ccggcatgcc cgtcctcaag aaggcagttg cagaaggcag
      781 cgccaccacc acagaaacca ccaacacgga aagtgcaggg taattcaagt ataaccctgt
      841 tcgacaacct ggcttttgcc gggaaaatat caacctgttc ttttagataa aaaggagact
      901 aattatgaca cgtacacagc atctaaaagc cactcaaacc aaaggggtgc agccatgcca
      961 gcattaaacc gcgttcagtt gatcggtcgt ttggggaaag acccagaaag taaatacacc
     1021 cccactggca agaaggtctg tcacttcagc ctcgccgtca gcaaccgttg gaaagacaag
     1081 aacggcgaga ccaaagaaac caccgaatgg gtcaacatcg aaacatgggg acggctgggc
     1141 gaagtttgcc aagaatattt gaaaaaaggc agcttgatct tcgtagaagg tcgcttgaaa
     1201 accgataagt acgaagatcg ggaaagcggc gaaacaaggt actacaccaa gatcattgcc
     1261 caaagcctgc aattcctcga tcgaaaagca agtgaagagc ctgttatggc ggtcgaagaa
     1321 gaatctggcg agtatgaact tgccatagag taataaatca ataaaaagac aactgccaaa
     1381 tggcagttgt ctttttatta caaatgggtg aatcttcttg cgcccctgtt ttggctggtt
     1441 tatacttgaa ctatgccact tccagaaaaa atcggtcgat acgaagttaa atcagaactc
     1501 ggacgcggcg gtatggctac ggtctaccgc gccgttgacc ccaactcggg gcgcgaggtt
     1561 gccgttaagg tcttgccgcg tgaaatgatg cacgacccgc aattccgcga acgttttgaa
     1621 cgcgagatca aaatgatcgc ctcattggaa catccagcca ttgtgcctgt atatgatgta
     1681 ggcgatattg acggtcagcc ttattttgtc atgcgcttca tgacgggcgg ctccctttcc
     1741 gatctcatcg aaaaaggcag tattccgatt gatgagacag caaagatcat cgaaaagatc
     1801 gctcagggct tggcatatgc ccatcgcaaa ggtgttatcc atcgcgatct gaagccagac
     1861 aatattctct ttaatgacga tggcgagccg ttcatctccg actttggtgt tgccaaatta
     1921 tctgagtcaa gcagcaatct aaccggtagc ggtgtgatcg gtactcctgc ctatatgagt
     1981 cctgaacagg ctcaggggaa tgaaatcgat caacgcagtg atgtgtatgg cttgggcgtg
     2041 atcgtctacc agatgttgag cggacatcag ccttacagcg
//
LOCUS       DAOREF010000608         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42653_length_2079_cov_4.396245, whole genome shotgun sequence.
ACCESSION   DAOREF010000608 DAOREF010000000
VERSION     DAOREF010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42653_length_2079_cov_4.396245"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="PKI33_12005"
     CDS             complement(<1..1757)
                     /locus_tag="PKI33_12005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNM37783.1"
                     /translation="MTVSASRWDLTNVYPSLDSKKFKAATKKYKSLLDELDAFLKKAE
                     KANSKTEPKKLGKLLGEAVDRFNELFELSGTMSSYLYSFITTDSRNKDAMRIFSEFQQ
                     MSVQGSILQTKFTAWAGTLGKPAIKKAVKTNASAKAHEFILLESAEQSKYLMSEAEET
                     LAAELTLSGGSAFGKLQGTLTSQMTVDFELDGEVKKMPMPALINLRSHPDESVRRRGY
                     EAENKAWADVQETLAACLNGVKGETLVLDRKRGREDNVHASIDAARIDRATLEAMLGA
                     MRDSFPMFRRYFNHKAKLIGKEKLAWWDLFAPLGKTDKVYSWDEARDFIVTNFNKFSP
                     ELGAFAQRAFDNNWIDGEQREGKRGGAFCMGVPAVKESRILANFDGSFDQVSTLAHEL
                     GHAFHNECAYQANKSELQQSTPMTLAETASIMCETIATEAALAETTDRDEVLAILEAQ
                     LNNASQVVVDIYSRYLFEKEVFERRAQSELSADEINDIMERAQKASFGDGLDEKYLQK
                     YMWTWKPHYYSAGFSFYNYPYTFGLLFATGLYAVYQKRGADFVPAYKELLATTGEANA
                     ADLADTFGINIRTKKFWAD"
     gene            complement(1839..>2079)
                     /locus_tag="PKI33_12010"
     CDS             complement(1839..>2079)
                     /locus_tag="PKI33_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNM37784.1"
                     /translation="PQGVLQDYARRYMELHEVEAKYVVSEQKTPRHLPDIAAEYNADL
                     ILMGSHSGNKLQQVLVGSMVDITLRESSIPAFICR"
BASE COUNT          461 a          500 c          578 g          540 t
ORIGIN      
        1 ctatctgccc agaacttctt ggtgcgaatg ttgatgccga aggtgtcggc aaggtcggcg
       61 gcgttggctt cgccggtggt agcaagcagt tctttgtagg cgggcacaaa gtctgcgccg
      121 cgtttttgat agacggcata caagccagtg gcgaataaca agccgaaagt gtacggatag
      181 ttatagaacg agaaacctgc agaatagtaa tgcggcttcc aggtccacat gtacttttgg
      241 agatacttct catccaatcc atcaccgaaa gaggctttct gggcacgttc cataatgtca
      301 ttaatctcgt cggctgaaag ctcagactga gcgcggcgtt caaagacttc tttttcaaac
      361 aaatagcgcg agtagatatc caccacaacc tgcgaagcat tgttcaactg tgcttcgaga
      421 atggcgagga cttcatctct atctgtggtt tcggcaaggg cggcttcggt ggcgatggtt
      481 tcgcacatga tggaagcagt ttcggcgaga gtcatcggcg tggattgctg tagctcgctc
      541 ttatttgcct gatacgcaca ttcgttgtgg aaggcgtgac ccaattcatg cgccaaggtg
      601 ctgacctggt cgaaggagcc gtcgaagttg gcaagaatgc ggctttcttt cacggcgggc
      661 acgcccatgc agaaagcacc gccgcgtttg ccttctcgtt gttcgccatc gatccaatta
      721 ttatcgaagg cacgttgagc aaaggcgccg agttcgggtg agaacttatt gaagttggtc
      781 acaatgaagt cgcgggcttc atcccacgaa tagactttgt cggtcttgcc aagcggagcg
      841 aatagatccc accaggcgag tttttctttt ccgatcaact tcgccttgtg attgaaataa
      901 cggcggaaca tggggaagga atcgcgcatg gcacccaaca tggcttcgag ggtggcacgg
      961 tcaatgcggg cagcgtcgat ggaagcgtgg acgttatctt cacgtccgcg tttgcgatcg
     1021 agaacgaggg tttcaccttt gacgccattc aaacaagcgg cgagagtttc ctgcacgtct
     1081 gcccaagcct tgttctcggc ttcgtagccg cggcggcgaa ccgattcatc ggggtgtgag
     1141 cgcaagttga tgagagccgg catgggcatc ttcttcactt cgccgtccag ctcaaagtcc
     1201 acggtcattt gggaggtaag cgtcccttgc agtttgccga aggcgctgcc accgctcaag
     1261 gtcaattcgg cggcgagggt ctcctcggct tcgctcatca agtatttgga ttgctccgcc
     1321 gattcaagca ggatgaattc atgcgctttg gcagaagcat tggtctttac cgcttttttg
     1381 atggcgggct tgccaagtgt ccctgcccag gcagtgaact tggtttgtaa aatgctgcct
     1441 tgcacgctca tctgctggaa ctcagagaaa atgcgcatgg cgtctttgtt gcgtgagtcc
     1501 gttgtgatga aagagtaaag ataggaactc atggtgccag aaagctcaaa tagctcattg
     1561 aagcggtcta cagcttcgcc gagcaatttg ccgagctttt tcggctcggt cttggagttg
     1621 gctttctcgg ctttcttgag aaaggcatcc agctcatcga gcagggattt gtatttctta
     1681 gtggcggctt taaatttttt ggaatcaaga gatgggtaga cgttggtcag gtcccaacga
     1741 gaggcagaaa cagtcattcg aaagctcctt gttgaaagat gggcttagta taccgtgatt
     1801 gatttttacc ttccccctgc tatggtaggg attaaaccct agcggcaaat gaaagcagga
     1861 atgctcgact cgcgcaaagt gatatctacc atgctgccga ctaggacctg ttgtagtttg
     1921 tttccgctgt ggctgcccat taggatcaag tctgcgttgt attcagcggc aatatctggc
     1981 aggtggcgcg gagttttttg ctcactcact acatacttgg cttccacttc atgcaattcc
     2041 atataccggc gggcataatc ttgcaaaaca ccttgcggg
//
LOCUS       DAOREF010000609         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42704_length_2078_cov_3.695502, whole genome shotgun sequence.
ACCESSION   DAOREF010000609 DAOREF010000000
VERSION     DAOREF010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42704_length_2078_cov_3.695502"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1227
                     /locus_tag="PKI33_12015"
     CDS             127..1227
                     /locus_tag="PKI33_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNM37785.1"
                     /translation="MSDFSHAYAVIMAGGGGTRLWPLSRKDKPKQLLPLIGQETLFQS
                     TVLRLKELFPPERILVVTIEEQAQEMKQQVPELPEENYLIEPAPRGTASVVGLAAMAL
                     QKRDPEASMAILPSDHFIRNVDLFHYLLKAAFDVAADNYLVTLGITPTMPSTGYGYIQ
                     QGTPIGGGYKYPVYTVQSFKEKPDEATAQQLLRMGGHSWNSGMFIWRADAILNEIDKQ
                     MPNLGKALREIGDAWNSSHKIETLKKIWPDLKNETVDYGIMEKAEKVAVLPAGGLGWS
                     DVGSWSSLFEVLLPDMSGNIATNDALHLALETNNTMVYGRGSDRLVVTIGVDDTVVVD
                     TGDVLLICKTDQSQKVKDVVEHLKKHRQEKYL"
     gene            1380..>2078
                     /locus_tag="PKI33_12020"
     CDS             1380..>2078
                     /locus_tag="PKI33_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNM37786.1"
                     /translation="MEAYCMKCKTKREMQDPVAGFNAKASAVTTGVCPVCGTKLFRMG
                     KTDAHAGLIPPPKPEKVEVREGKLVIVESPAKAKTVGRFLGKGYTVRASVGHVRDLLK
                     SQLSVDVDNNFEPKYRVPNEKKDVVKEIKQLAKKAEEIFLATDPDREGESISWHLMEA
                     AGIEPERTKRVVFHEITAPAVADAFSHPRNIDMNLVNAQQARRVLDRLVGYSISPILW
                     EKVRGRLSAGRVQSV"
BASE COUNT          558 a          429 c          542 g          549 t
ORIGIN      
        1 caattgagaa aatttcagcc gcattagcta tgatggaatt gaaaggtatg gttcggcaag
       61 tcggtggtat gaattacgtg gctgtccgtg atggacagtc tgattacatg gtttaggaga
      121 aaagctatgt cagatttttc acatgcctat gcggtgatta tggcaggcgg tggtgggacg
      181 cgtttgtggc ctctttcccg caaagataaa cccaagcagc ttttaccttt gattgggcag
      241 gaaactcttt ttcaaagtac ggttttgcgt ttgaaagaat tgttcccacc cgaacgcatt
      301 ttggttgtga caattgagga gcaggcacag gaaatgaagc agcaagttcc tgaattgcca
      361 gaagaaaatt atttaatcga acctgctccg cgtggaacgg cttcagttgt agggttagca
      421 gccatggcat tacaaaaacg agaccccgaa gcctccatgg caattttgcc atctgaccat
      481 ttcattcgca atgtagattt gttccactat ctacttaaag ctgcttttga tgttgcagcg
      541 gataattacc tggtcacact tggcattacg ccgaccatgc cttctactgg gtatggatat
      601 attcagcaag ggacgcctat tggcggtggt tacaaatacc ctgtttatac agtgcaaagt
      661 ttcaaggaaa aaccagatga agccactgcg caacaattgc ttcgtatggg tgggcattct
      721 tggaatagcg gtatgtttat ttggcgtgcc gatgcgatct tgaatgagat tgacaaacaa
      781 atgcctaatt taggtaaagc cctgcgtgaa attggtgatg cgtggaattc ttctcataag
      841 attgagacct tgaaaaaaat ctggcccgac ctgaagaatg aaactgtgga ctatggcatt
      901 atggaaaaag ccgaaaaagt tgccgtcttg cctgctggcg gtttggggtg gagcgatgtt
      961 ggttcatggt cttcgctatt cgaagtcctt ctgcctgata tgagtggcaa tattgccacc
     1021 aatgatgctt tgcatttagc gcttgaaacc aacaacacaa tggtctacgg acgtggatct
     1081 gatcgcctcg ttgtgaccat tggagtggac gatacagtgg ttgtagatac tggcgatgtg
     1141 ctgttgatat gcaagacaga ccagtcacaa aaggttaaag atgtagtgga acatctcaaa
     1201 aaacatcgcc aagaaaagta cctttaaaat cgcccgaaaa attggcgttt gcaaaaaaac
     1261 ggaaacttgt tgtagaatgt ccatcccgta aggtttaacc ttgcgggatg ttttttatac
     1321 gaaccgaaca actctcgagg caaattctgc tttgtgagag atatgttgga ggaatacaaa
     1381 tggaagctta ttgtatgaag tgcaagacca aacgggaaat gcaagatcct gtggcagggt
     1441 tcaatgccaa agcatctgca gtgacaacag gtgtctgccc cgtttgcggc acaaaattat
     1501 ttcgcatggg aaagaccgat gcccatgctg gtttgattcc gccacccaag cctgagaagg
     1561 tggaagtgcg cgagggcaag cttgtaattg ttgagtcgcc tgccaaggca aaaacggtgg
     1621 ggcgttttct tggaaagggc tataccgtcc gtgcttcggt ggggcatgtg cgcgaccttt
     1681 tgaagtctca gctttctgtg gatgtggata ataatttcga gccgaaatat cgcgttccca
     1741 atgaaaagaa agatgttgtc aaagaaatta aacagcttgc gaaaaaagcg gaagaaatct
     1801 ttctcgcaac cgatcccgac cgcgaaggtg agtcgatttc atggcatctg atggaagcag
     1861 ctggcattga gcccgagcgt accaaacgcg tggtcttcca tgaaattaca gcgcctgctg
     1921 ttgcagatgc tttctcgcat ccgcgtaata ttgacatgaa cttggtcaat gcccaacagg
     1981 cgcgccgcgt gctggatcgc cttgtgggct atagcatcag ccctattttg tgggaaaaag
     2041 tccgcggacg cctctctgca ggtcgtgtgc agtctgtc
//
LOCUS       DAOREF010000610         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42826_length_2075_cov_3.618812, whole genome shotgun sequence.
ACCESSION   DAOREF010000610 DAOREF010000000
VERSION     DAOREF010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42826_length_2075_cov_3.618812"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="PKI33_12025"
     CDS             complement(<1..1812)
                     /locus_tag="PKI33_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNM37787.1"
                     /translation="MQKAKNVSGIIRPMSTWSGKTLGNVLIGDLIARGGMAEVYYGVH
                     STLNRKVAIKIMRDHVEEDPDSRARFAREARVVATLDHPNIIQIYDYDLVDGRPCIVM
                     ELVEGTSLAGYLKELHKRGEKLPHPIVAKLMKSIASAMDHAHERNIVHRDIKPANILL
                     RSKQGVIDLQAPLPEDVEPVLTDFGLVRLLDSNTKTSTGTVSGTPSYMSPEQARGDSV
                     DHRTDIYSLGVVLYEMLAGEVPFDAESSFGVLMKHLNEPPPPINGISAGMQAIINRAL
                     AKDPKDRFASAIEMVNEFADVLEGKSVSKETLRINSLPKETTANAKQATSSFKTPLLV
                     GGGIIALALIVSLGVFIFRQPRETNVPSQNNNQLPNPSQPIGSVVFDDYNELADNVTV
                     TISGIPLPPAGQHFEVWFLGQGGEIKLNAGPVIFTGQNGQLIFSDSNGANLLSQFDEM
                     MVTVEPDNDPNPDQPSQEVAASSVFPPQAFVHIRHILVSIGMTPNGTALIQGVWDTAD
                     TIDTSVLELRDAFNEGDEATLRIKNEEIINQLVGEENLQLYKDWNGDGEVNDPNDGFG
                     LLANGIEQGYIPLTISHTEFAIDAPDATTGIIQSGQNL"
     gene            complement(1872..>2075)
                     /locus_tag="PKI33_12030"
     CDS             complement(1872..>2075)
                     /locus_tag="PKI33_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37788.1"
                     /translation="VHAFGQKYIFEIRDSRVTKPFATSYAFEHLEDESYLTLITCQVY
                     LPKSDTYLYRRVVRAVLVSVTSE"
BASE COUNT          510 a          454 c          529 g          582 t
ORIGIN      
        1 taagttttgt ccgctttgga tgatgcctgt ggtcgcatcg ggagcatcta tagcaaattc
       61 tgtatggctg atcgtcaagg ggatgtagcc ttgttctatg ccatttgcaa gtagcccaaa
      121 cccatcattg gggtcgttga cctcaccatc tccattccag tctttataca actgaaggtt
      181 ttcttctcct acaagttgat taataatttc ttcattcttt attcgcagag ttgcttcatc
      241 gccttcattg aatgcatctc taagctctaa aacagatgta tcgattgtat cggctgtgtc
      301 ccatacgcct tggatgaggg ctgttccatt gggcgtcatg cctatggaga cgagaatatg
      361 ccttatatgg acgaacgcct gcggagggaa caccgaagag gcggcaactt cctggctggg
      421 ttggtcgggg ttgggatcat tatccggctc taccgtgacc atcatctcat caaactgact
      481 gagaagattc gcaccgtttg agtctgagaa aattaattgt ccattttgtc cggtaaatat
      541 caccggaccc gcatttagtt ttatttctcc gccttgaccc aggaaccaga cttcaaaatg
      601 ttgcccagct ggcggtaggg ggattccgct gatggtaact gtgacattat cggccaactc
      661 attgtaatca tcaaagacaa cggaaccaat gggttgactt ggattgggta attgattgtt
      721 attttgggat ggtacgtttg tttcacgcgg ctggcggaag ataaacacgc cgagtgaaac
      781 gattagggca agggcaatga tccctccgcc gaccaatagg ggagtcttga acgatgaagt
      841 ggcttgtttt gcatttgccg ttgtttcttt tggcaaacta ttgattctta atgtctcttt
      901 tgatacagat ttaccttcaa ggacatcggc aaattcattg accatttcaa tcgctgaagc
      961 aaaacgatct ttgggatctt ttgcaagtgc gcgattgata atggcttgca tacccgctga
     1021 aataccatta atcggtgggg gcggctcatt taggtgcttc attagaacac caaatgaaga
     1081 ctccgcgtcg aaggggactt ccccggcaag catttcgtaa agaacaacac ctagagagta
     1141 gatatctgtt cggtgatcaa cactatctcc acgggcttgt tcagggctca tgtaagaagg
     1201 ggttccggag acggtaccag ttgaggtctt cgtattcgag tcgaggaggc ggaccaagcc
     1261 aaagtcggtt agaaccggtt ctacatcctc tggaagggga gcttgtaaat cgatgacacc
     1321 ttgtttggac cgaagcagaa tattggcagg tttgatatcg cggtgcacaa tgttgcgttc
     1381 atgtgcatgg tccatggctg aggcgatgga tttcatgagt ttggcaacta tggggtgtgg
     1441 aagcttttcg ccgcgcttgt gcaattcttt tagatagcca gctaaagagg ttccttcgac
     1501 aagttccatg acaatgcatg gacgcccatc gaccaaatca taatcgtaga tttggataat
     1561 atttgggtga tcgagggttg ctacaacacg tgcttcacgg gcaaaacgtg cgcgcgaatc
     1621 gggatcttct tccacatgat cacgcatgat cttaattgca actttgcgat tgagtgtgga
     1681 atggacgcca taatagactt ctgccatgcc tccgcgtgcg atgaggtcac cgattaaaac
     1741 attgccgagg gtttttccgc tccaagttga catggggcgg attatacccg atacattttt
     1801 ggctttctgc ataataaaag acatccgaag ctttcgcctc ggatgtcttt tgtaactact
     1861 tcagggagga cttactcgct tgtcacactg accaatacgg cacgaacaac acggcgatag
     1921 aggtaggtgt cactcttagg taggtatacc tggcaggtga tcagggtgag ataagactca
     1981 tcttcaagat gctcgaaagc atagcttgtg gcgaagggct tagtcacacg agagtctcgg
     2041 atctcgaaga tgtatttctg cccaaaggcg tggac
//
LOCUS       DAOREF010000611         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42828_length_2075_cov_3.242079, whole genome shotgun sequence.
ACCESSION   DAOREF010000611 DAOREF010000000
VERSION     DAOREF010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42828_length_2075_cov_3.242079"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..862
                     /locus_tag="PKI33_12035"
     CDS             149..862
                     /locus_tag="PKI33_12035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM37789.1"
                     /translation="MDANITKQLIELNRKFYDQFGESFSATRQRLQPGVKKILETIHA
                     DESVLDLGCGNGFFLRELYDRDHHAPLLGADFSLPLLREAESTPGVEFRELDLMQLSA
                     FSNQLSVAGGWDVITMFATMHHIPSREVRLDILHTVKKLLKPNGKFILSNWQFLNSEK
                     LKARVQPWGKAGLSDSDVDEGDYLLDWRSGGEGLRYAHQFSEQELLGLAEQAQMKVEA
                     SFLSDGENGKLGLYQIWRA"
     gene            complement(859..>2075)
                     /locus_tag="PKI33_12040"
     CDS             complement(859..>2075)
                     /locus_tag="PKI33_12040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM37790.1"
                     /translation="YNTPYASASVVSRFPAKSLLILPLIALEKKLGAVLVSFETPHRF
                     IEEEVQICEQAASLIALALEKFQAMDEAQRRANTSESLRRVGIAVAEQTELKQAVNYV
                     LEQLKQMVPYDSASVQLRNGDELYIIGGQGWGDEQEIIDLRFPIPGDNPNTVVIERGS
                     PYYLPDAGEVYKQFRKPPHNHIRSWLGIPLLVQEKIIGLLAIDSAEPNDFSEQEIMIA
                     TEFANQVAVTLENARILKETQTQAVTDPLTGISNRRGLIEAGKVEFSKTHTTNRPFSA
                     IMLDLDYFKSINDTYGHAVGDLVLREFAKRCKSCIREIDYIGRYGGEEFIILLPDAHM
                     KASMNIAERLRSAITKKPIRIDDKEISISASLGVACRDENTTSLETLIARADQALYIA
                     KHNGRNCVAVSH"
BASE COUNT          520 a          452 c          510 g          593 t
ORIGIN      
        1 ctcatggcga tatttggcgc gtattcttta atagcccaac ggatgatctc ttgcggggtt
       61 ttggtttcaa attctaaaga gagtttttga atatcttgtg cagaaagcat gaaaaaatta
      121 taccgttcta aaccgttata atttccctat ggatgccaac attacaaaac agttaattga
      181 actgaaccgc aaattttatg accagtttgg cgagtcgttt tctgcaacac gtcagcggtt
      241 gcagcctggg gtgaagaaga ttcttgaaac gatccatgcg gatgaatctg tgttggactt
      301 gggttgtggt aacggatttt ttctacgtga gttgtatgat cgcgatcacc atgccccgct
      361 tcttggcgca gactttagcc tgcctctgct acgagaagcc gaatccacgc cgggagttga
      421 gtttcgagag ctggatctca tgcagctttc agccttcagc aatcagcttt cagtggcagg
      481 cggctgggat gtgatcacga tgtttgcaac tatgcatcac atcccttcgc gagaagtccg
      541 cttggatatt ttgcacacgg tgaaaaaatt actcaagcca aatggaaaat tcatcctttc
      601 gaattggcaa ttcttgaaca gcgaaaagtt aaaagccaga gtccaaccgt gggggaaggc
      661 agggctttca gattcagatg tggatgaagg ggattatctg ctcgattggc gcagtggtgg
      721 tgaagggttg cggtacgcgc atcaattctc agagcaggaa ctacttgggt tggcagagca
      781 agcccaaatg aaggtggagg cgtcttttct atccgatggg gaaaatggca agttggggtt
      841 gtatcaaatt tggcgggctt aatgactgac cgctacgcaa tttcgcccat tatgcttagc
      901 aatatataaa gcttggtccg cacgggcaat caacgtttcc agcgatgttg tattttcatc
      961 tcggcatgcc acgccaaggc tggcagagat actgatttct ttgtcgtcaa ttcgaatcgg
     1021 ctttttcgtg atggcacttc gcaaacgttc ggcgatgttc atgcttgctt tcatgtgagc
     1081 atctggtagc agaatgatga attcttcgcc gccatatcgc ccaatgtaat ctatttcgcg
     1141 gatgcaactt ttacaacgct tggcgaattc gcgcaaaaca agatcgccca ccgcgtgccc
     1201 atacgtgtca ttgatagact tgaaataatc aaggtctagc atgatcgctg aaaacggacg
     1261 atttgtagta tgtgtttttg aaaattcaac cttgcccgct tcaatcaacc cgcggcgatt
     1321 gctaatacct gtaagcgggt cggttactgc ctgcgtttgg gtttctttca atatcctagc
     1381 gttctcaagt gtgacggcaa cttgattggc aaattccgtg gcgatcatga tctcttgttc
     1441 gctaaaatca ttgggctcag cactgtcgat ggcgagcaat ccaatgatct tttcctgtac
     1501 aagtaaggga atgccaagcc acgaacggat gtggttatgc ggaggttttc tgaattgttt
     1561 gtatacctca cctgcgtctg gtaagtaata tggagaccct cgttctataa caactgtgtt
     1621 ggggttatct cctgggatgg ggaagcgtag gtcaataatt tcttgctcat cgccccaacc
     1681 ctgacctcca ataatataga gttcatctcc attacgaagt tgtacagaag cactatcgta
     1741 tggcaccatt tgtttaagct gttcaagaac atagttgacc gcctgtttaa gctcggtctg
     1801 ttcggcaaca gcgatgccca ctcttcgcag actctctgat gtgtttgcgc gccgctgggc
     1861 ttcatccatg gcttgaaatt tttcaagagc cagagcgatg agggaagctg cttgttcgca
     1921 gatttgtact tcttcctcaa taaagcgatg aggagtttca aatgaaacta gaacggcccc
     1981 caattttttt tctaatgcaa ttaatggcaa aatcaaaagc gattttgctg gaaaacggct
     2041 aaccaccgaa gccgaggcat agggtgtgtt gtaaa
//
LOCUS       DAOREF010000612         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42866_length_2074_cov_3.219416, whole genome shotgun sequence.
ACCESSION   DAOREF010000612 DAOREF010000000
VERSION     DAOREF010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42866_length_2074_cov_3.219416"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2074
                     /locus_tag="PKI33_12045"
     CDS             <1..>2074
                     /locus_tag="PKI33_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM37791.1"
                     /translation="ALLMLRLVARTHSDPQESLASPVLTKSNSRDAVIVIQPGGRVEY
                     LSAAARPYFNLREDEPFDLERLARHVRPSDDFIDLCVTPGIRRVSINGKPIELSSYEV
                     PGAYPRMLISVRGKDASAASISNNSSESDEILRVATEFGQTIAASLDLDTTVRSILDH
                     VGRLVPSDVLELKLWNEERQILTPFRFQQAASSKVITASLSRFGGLTDQLSARREPII
                     LNDFESIHEVLSGNELNPIHSYMGIPLMAGGELVGTLEAGQLSSGAFGQHDLNLLHLI
                     SGQAAIAIRNARLYEDEQRRRAELTGLANLNQALGAVRDGQDVIAQLVESIAPLFDAE
                     IVGFLLYDENKRTLEGRNPFRGLPAHFVEIYRATILADSPAEAVITRQEPVITTNAMA
                     DESWRALGLADIATAASLRDIALIPLLSSGRMLGYLQVGHHARGVVSFNAEELRLMSI
                     VANQSASIIANILLVQQARTRAQRADALRRIASLAASSATLDEILKYSVLELARLFNA
                     DRGAVFLLDEARGMLILNSPSMYGVPPEIGDNFIQIFLDDPDFRHTVSGGKRPFLSGH
                     LSSERRILPAYRPLATALAMESAMVVPLVVRDRSVGELMLGSKKQDHFNSSDLQAVST
                     AAGQLAAAIESAGLLSQTDNTLRHRVDQLSFITRVNRELISSLDIRHLLGVLREEAVR
                     ISQADCASA"
BASE COUNT          443 a          500 c          538 g          593 t
ORIGIN      
        1 gctcttttga tgctacgcct tgttgcccgt acccattcag acccccagga aagtttagct
       61 tcccccgttc ttacaaaatc aaattcgcgc gatgcggtga tcgttattca acccggtgga
      121 cgggtggaat atcttagtgc ggctgcacgc ccttacttca atttgcgtga agacgagcct
      181 tttgaccttg aacgcctagc acggcatgtg cgtccttcgg acgatttcat tgatctgtgt
      241 gtaacgccag ggatacgacg cgtttctatc aatggaaagc caatcgagct ttcttcttat
      301 gaggtgccgg gggcatatcc gcgcatgttg atttctgtgc gtgggaaaga tgcgagcgct
      361 gcaagcatct caaataattc atctgaatcg gatgaaatct tgcgtgtggc aacagagttt
      421 ggtcagacca ttgctgctag cttagacttg gatacaacgg tccgctcgat tctcgaccat
      481 gttggacgtt tggttccttc agatgttttg gaattgaagc tttggaatga agaacgccag
      541 atcctgactc catttcgctt ccagcaagcg gcttcgagca aagtgattac ggcgtcgctt
      601 tcgcgctttg gtggtcttac cgaccagtta tcggcgcgga gagaaccgat cattcttaat
      661 gattttgaat ccattcacga agttttatca ggtaatgaat taaatcccat tcattcgtat
      721 atgggcattc cgctcatggc tggtggggag ctggtgggta cattggaagc tggtcagcta
      781 agtagcggtg ctttcggtca gcatgacttg aatcttctgc atttgatctc ggggcaagca
      841 gcgattgcca ttcgtaatgc aaggctgtat gaagatgaac aaagacgccg cgccgaattg
      901 acagggctcg cgaatttaaa tcaagcgctt ggggctgtcc gcgatgggca ggatgtaatt
      961 gcgcaattgg tagaaagcat cgctccgctg tttgatgcgg agattgttgg ttttcttcta
     1021 tatgatgaaa ataaacgtac cttggaagga cgaaatccct tccgtggttt gcctgcccac
     1081 tttgtagaaa tttatcgtgc cacgattttg gctgatagcc ctgccgaagc tgtcatcacg
     1141 cgtcaggagc cggtgatcac aaccaatgcc atggcagatg aaagttggcg tgcgttaggc
     1201 ttagcggata ttgcaactgc ggcaagtttg cgcgatattg ctctcattcc gcttctttct
     1261 tcgggacgca tgctcggcta tcttcaggtt ggtcaccacg ctcgaggggt ggtttctttc
     1321 aatgctgaag aattgcgctt gatgagcatc gttgccaatc agtctgcttc gatcatcgcg
     1381 aatattctgc tcgtgcagca agcgcggact cgtgctcagc gtgccgatgc attgcgtcgc
     1441 attgcaagtt tggcggcatc ttctgccacc cttgatgaaa ttctaaaata ttctgttctg
     1501 gaattggcgc gccttttcaa tgcagaccgc ggtgcggttt tcttgttgga tgaagcccgt
     1561 ggtatgttga ttcttaattc tccatccatg tacggagttc cacctgagat cggggataac
     1621 ttcatccaaa tattcttgga tgacccagat tttcgccaca ccgtttctgg tggtaagcgt
     1681 cccttccttt cggggcatct cagctcagag cgccgtattc tgcccgcata tcgtccactc
     1741 gccaccgcac tcgccatgga atctgccatg gtcgttccgt tagtggttcg cgaccgcagt
     1801 gtgggtgagt tgatgctggg tagcaagaaa caggatcact tcaattcatc ggacttgcaa
     1861 gcggtttcca cagcagcagg tcagttggcg gcggcgatcg agtctgcggg tttgttatct
     1921 caaacagata acactctccg tcaccgtgta gaccaacttt ccttcattac acgtgtaaat
     1981 cgtgaactaa tttcttccct cgatattcgt catctgttgg gtgttttaag agaagaagct
     2041 gttcgtatct cccaagccga ttgcgcttcg gcag
//
LOCUS       DAOREF010000613         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_42923_length_2072_cov_4.660387, whole genome shotgun sequence.
ACCESSION   DAOREF010000613 DAOREF010000000
VERSION     DAOREF010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42923_length_2072_cov_4.660387"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PKI33_12050"
     CDS             complement(<1..1337)
                     /locus_tag="PKI33_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37792.1"
                     /translation="MKPTILIVDDEPSGQQVVESILQDQGYTMEFASTGKEAIKKAGE
                     LKPDLILLDIMLPEIDGIQVCQTLRKNTELAKIPVVMLTALNDRDIRIASLDAGADDF
                     ISKPFDRAELRARVRSITRLNTYRLLYERNMMFAWISDQSNDGYLLVRNGDEVIYANP
                     RARFYLGMDLDVNIPSGSSFMGIASRQYRPQPIEAWKTWPKPASSNEPRVRYLVRPET
                     TSSHEFWLEASVFETPKGEVGSRIIRLRDVTADILNRRNTRGFGEAITHKIRTPMTHM
                     ISSLDLLVKHLPKLSQEEAMHLLETAFMGAKRLSDTMDRILKYSNLYTYTNNSECFSL
                     SYFNEVVERIAGEAGIENIQIQMDESISQTKIVLPLQSVEVLLWEILDNSKKFHPSQM
                     PAVKIDVLMNASKNIQIQISDDGMALSPKQLTTAWLPYYQGEKDFTGEMPGMG"
     gene            complement(1465..>2072)
                     /locus_tag="PKI33_12055"
     CDS             complement(1465..>2072)
                     /locus_tag="PKI33_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37793.1"
                     /translation="GIRALLEKVGDMVILGEASNGQEAIDMTESLNPDVLIMDIMMPR
                     LNGIQAAEQLRSRKHRVNILLLSMYADEGLVYQALQSGANGYVLKSSASDELVEAVRE
                     IANGKKFLSGPISEIVMENAFKPRPANEVDPLASLSPREKEIMQLIAEEHTSVEIAQF
                     LSISEKTVEKHRANLMEKLHVRNLAGLVRLAVKYRLVDRDS"
BASE COUNT          598 a          499 c          378 g          597 t
ORIGIN      
        1 aaccccatgc ctggcatttc gcctgtgaaa tctttttcgc cttgataata tggcaaccat
       61 gcagtcgtta attgtttcgg cgacagagcc attccatcgt cactgatctg aatttggata
      121 ttttttgagg cattcataag tacatcaatt ttcacagcag gcatttgaga tgggtgaaac
      181 ttttttgaat tatccaaaat ctcccataac aagacttcaa cagactgcaa tggcagaaca
      241 atctttgtct gacttataga ttcgtccatt tgaatctgta tattctctat ccctgcttcg
      301 cctgcaattc tttccaccac ttcattgaaa taagataaag aaaagcactc tgaattattg
      361 gtataggtat aaagattcga atattttagt atcctatcca tcgtgtcaga taaacgctta
      421 gcacccataa atgcagtctc taacaaatgc attgcctctt cttgagataa ttttggcaaa
      481 tgctttacaa gcaaatcaag gctcgaaatc atatgcgtca ttggggttcg tatcttatga
      541 gtgatcgctt ctccaaaccc tcgagtgtta cgtcggttta ggatatcagc cgtcacatca
      601 cgaagacgaa taatgcgaga cccaacttca ccttttggcg tctcaaaaac cgatgcttcc
      661 aaccagaatt catgagaact cgttgtttca ggacgaacca gataccgaac acgcggctca
      721 ttactagacg caggcttggg ccaagttttc caagcttcta ttggctgtgg gcgatactgc
      781 cgagaagcaa ttcccataaa ggaactacca gatggaatgt tcacatccaa atccatacca
      841 agataaaaac gggctctcgg gttggcataa atgacctcat ccccatttcg aactagcaaa
      901 tatccatcat tcgattggtc gctaatccac gcaaacatca tatttcgttc atataaaagg
      961 cgataggtat ttaggcgcgt aatcgaacgt acacgagcac gtaactctgc ccgatcaaaa
     1021 ggcttggaaa taaaatcatc agctccagca tccaaactgg caatacgaat gtcacggtca
     1081 ttcaaagcag tcaacatcac aactggaatc ttggcaagtt cagtgttttt acgcaacgtc
     1141 tgacaaactt gaatgccgtc tatttcaggc agcataatat ctaaaagaat cagatccggc
     1201 ttcaattccc ctgctttttt gatggcttcc tttcctgtgg aagcaaattc catcgtatac
     1261 ccctgatctt gcaatataga ttcgactact tgttgcccag agggctcatc atcaactata
     1321 agaatggtag gtttcattca tatctgtcct caaacataaa tatggataca tatactacta
     1381 acctaaccaa ggatatttca acttatccta cttgaggata caggaaaacc cctattgtga
     1441 agaggcttcc agtattgtaa acaactagga atccctgtcg acgaggcgat atttcactgc
     1501 aaggcgcacc aaccccgcca ggtttcgtac atgtaatttt tccatcaagt ttgcacgatg
     1561 tttttccact gttttttcac taatcgagag gaattgtgca atctccacac ttgtatgttc
     1621 ttctgcaatt agttgcataa tctccttttc acgaggagat aaactagcaa gcggatccac
     1681 ttcatttgca gggcgcggct tgaaggcgtt ttccatcacg atttcagaaa tgggaccact
     1741 cagaaacttt ttgccatttg caatttcacg cacggcttct accaattcat cgctggcaga
     1801 agatttcaac acatatccat ttgcgccact ttgcaaagct tgatatacca agccctcatc
     1861 tgcatacatg gatagcagca ggatattgac ccggtgcttt cgactccgca gttgttctgc
     1921 tgcttgaata ccatttagac gaggcatcat gatatccata atcagaacat ctggatttag
     1981 agactcagtc atatctatgg cttcctgccc atttgaggct tcaccaagaa taaccatatc
     2041 gccaactttc tctaacagtg cacgaatccc cg
//
LOCUS       DAOREF010000614         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43139_length_2066_cov_4.905520, whole genome shotgun sequence.
ACCESSION   DAOREF010000614 DAOREF010000000
VERSION     DAOREF010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43139_length_2066_cov_4.905520"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKI33_12060"
     CDS             complement(<1..426)
                     /locus_tag="PKI33_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HNM37794.1"
                     /translation="MTSIVLILFLLALMFFWQSNRQQKAAGLPGGRVIYTDTRGWGKV
                     EKPLFYPALGLTGKPDYLIEKNGQIIPVEVKSGRAPEAPYDSHIFQLASYCLLVEKTY
                     GRRPPYGIIHYENRDFAIDYTRELETALIELIADMREDDV"
     gene            complement(423..677)
                     /locus_tag="PKI33_12065"
     CDS             complement(423..677)
                     /locus_tag="PKI33_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37795.1"
                     /translation="MCYTTQMRPIRSSEIGSYLYCRRAWWYRKIGTPSENKAELAAGT
                     ELHRKHGRQVLASALTRTLGWLLLLAAILTLVAYLAFQVL"
     gene            758..1309
                     /locus_tag="PKI33_12070"
     CDS             758..1309
                     /locus_tag="PKI33_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNM37796.1"
                     /translation="MTKQDTYSIDIAGVKRELRLFEIKPGLRIAILNILGDTELVQAC
                     ARGLADKVKNVEYDIIVTAEAKSIPIAHALSVETKKPYVVLRKTYKPYMGDALKAETL
                     SITTGQPQVLILDEKDRELIQGKKVLVVDDVISTGSTLQGMRMILDKANATVAGEAAI
                     LTEGDRSQWMNIHSLGHLPLFTD"
     gene            complement(1338..1580)
                     /locus_tag="PKI33_12075"
     CDS             complement(1338..1580)
                     /locus_tag="PKI33_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNM37797.1"
                     /translation="MSCFCYILECADGTFYTGWTTDPERRVSQHNKGVGARYTRTRRP
                     VRLVYLEEQPDKITALKRERAIKALSRKKKMELFMM"
     gene            complement(1577..>2066)
                     /locus_tag="PKI33_12080"
     CDS             complement(1577..>2066)
                     /locus_tag="PKI33_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37798.1"
                     /translation="VKLLGSTSILTDVVESDGLMASSYILPRLASVALLNGTMSQPGW
                     NEVKKSASRGYFAATSALLDNSLEGLAMLAEHNRANTIHSLNVMINSLLDLRDDLENE
                     DHASFVKRLKSAKEGRESWLVEREKADWSRIPGEEIEKPSFMESLFGSKLGKLGKDKG
                     KG"
BASE COUNT          499 a          497 c          559 g          511 t
ORIGIN      
        1 gacgtcgtct tcgcgcatat cggcaatgag ttcaatcagc gctgtttcca gttcacgagt
       61 gtagtcaatg gcaaagtcgc ggttctcgta gtggatgatg ccatagggtg gacgcctgcc
      121 ataggtcttt tcaacgagca gacaatagga agctaactga aaaatatgcg agtcatacgg
      181 cgcttcaggc gcacgtcctg acttgacctc cacggggata atctgcccgt tcttttcgat
      241 caggtaatcg ggtttgcctg tcagcccgag agcggggtag aacagaggct tttccacttt
      301 gccccaaccg cgggtgtcag tgtagatgac acgtccgccg ggcaagccgg cagctttctg
      361 ctggcggttc gattgccaga agaacataag cgcaagcaaa aaaaggataa ggacgatgga
      421 tgtcatagca cttgaaaagc aagatacgct accaaagtaa gaatcgctgc tagaagcagg
      481 agccagccca aggtccgcgt gagagccgag gcaaggactt ggcgcccgtg tttgcggtgg
      541 agttcagtgc cggcggcaag ctccgctttg ttctcggagg gtgtcccgat cttgcgatac
      601 caccaggcgc ggcgacagta gaggtaagaa ccaatttcag aggagcggat cggtcgcatt
      661 tgggtagtat agcacaaatt ttctaattga attgaggcgg gaacataatc gaatcgtgat
      721 aaactttcgg tgcaaaattt ccccgtggag acgtctgatg acgaaacaag acacttactc
      781 aatcgatatt gctggcgtaa aacgcgagtt acgcttgttt gaaattaaac cagggttgcg
      841 cattgcgatc ctgaatattt tgggcgacac cgaattggtg caagcctgtg cccgcggact
      901 tgccgataag gtgaagaatg tcgagtacga cattatcgta accgctgaag cgaagtccat
      961 tcctattgcg catgcgcttt cggtggagac caagaagccc tatgtggtgt tgcgcaagac
     1021 ctataaaccg tatatgggtg atgccctcaa agcagaaacg ctttcgatca ccacgggtca
     1081 accgcaagtc ttgatcttgg atgagaagga ccgtgagttg attcaaggca agaaggtttt
     1141 ggtagtggat gatgtcatca gcaccggttc gaccctgcag ggcatgcgca tgatcctcga
     1201 taaagccaat gcgactgtgg ctggtgaagc ggcaatcctc accgagggcg accgttccca
     1261 gtggatgaat atccattcgc tggggcattt acctttgttt actgattaaa tgcgtaaggg
     1321 cgactcgcga gtcgccccta catcatgaac aattccattt tcttttttct tgagagagcc
     1381 ttgatggctc tctctcgttt taacgctgtg attttatcgg gctgttcttc gaggtagacc
     1441 agtctcacgg ggcggcgggt tcgggtgtag cgtgcgccaa cgcctttgtt gtgctgagac
     1501 acgcgtcgtt ctgggtcggt ggtccagccg gtgtaaaagg ttccgtcggc acattcaagg
     1561 atgtaacaaa agcagctcat cccttgcctt tatcttttcc caacttgcca agttttgagc
     1621 caaacagact ttccatgaaa gatggctttt cgatctcttc cccaggaatt ctcgaccagt
     1681 ctgctttttc tcgttcgacg agccaacttt ccctgccttc cttggcagac tttaatcgct
     1741 ttacgaaaga agcatggtct tcattttcaa ggtcatcgcg caggtcgagc agactgttga
     1801 tcatcacatt caacgaatgg atggtatttg cgcggttgtg ttccgcgagc atggcaagcc
     1861 cttccaacga attatctaat aaggcagagg tggcggcgaa atatccgcgg ctggcagatt
     1921 ttttcacttc gttccaccct ggctgactca tcgttccatt caataaagca acagacgcca
     1981 aacgtggcag gatgtaagaa gaagccatca acccatcgga ttcaacgaca tcggtcagaa
     2041 tggaggttga gccgaggagt ttgacg
//
LOCUS       DAOREF010000615         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43242_length_2063_cov_4.682769, whole genome shotgun sequence.
ACCESSION   DAOREF010000615 DAOREF010000000
VERSION     DAOREF010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43242_length_2063_cov_4.682769"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKI33_12085"
     CDS             <1..386
                     /locus_tag="PKI33_12085"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNM37799.1"
                     /translation="WRKGLAVTAEAFKGKEIRGRAVLVHTGWAEHWATDAYFENHPFL
                     TEDAAIYLRDNGAKLVGVDSHNIDDTRGNSRPVHSVLLKNEIIVAEHLCNLEKVPDER
                     FTFTAAPPKVKGAGTFPVRAFAKVN"
     gene            complement(453..>2063)
                     /locus_tag="PKI33_12090"
     CDS             complement(453..>2063)
                     /locus_tag="PKI33_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM37800.1"
                     /translation="PRLTEHFDIVFFDQRGINQSGGLYCVNAVTKYYSTNTDATTPEG
                     EAQLLDVTKTFVDECIAEVGQTDLLPYLDTTQAVEDLEVFRGLMGDDKFWLYGESYGT
                     QFSQMYATAHPDHLAGLILDGTVDITLTDTEFLKGQAAAFNDVMVATLEACNADEACS
                     ADMGRDAVAVYDELAAQLKQESIKFDFPLSTGEVQPREFSFSDLEATSGFLYSETARM
                     MFLRALAAYARDDTLVPLARVVYNSLYINPDTLDAVLDPSWSDAIYYGVTCRDYAEPG
                     STFDEQAELFLREGDSVDATLPRFSSVFYGDLPCVYWPYPKDDSQPAMLPSPFTGSGV
                     PTLVLNATTDPATPYEGAKNVFSHLDNGYMVTEIGGPHVIFGWGNECVDGLVTDFLVD
                     DKMPERETTCEGYVADDFVPLALADASEYANPLEALVAVDNEINYLPEYYYSISEEIN
                     AAACPYGGNVTFESGDTSDTYTLTNCAFSAGFTMTGTGSYNYDDGSFTLEVSVTGLKD
                     GTLTYIRDAEYAIHVTGEYDGDVIDLSE"
BASE COUNT          501 a          486 c          584 g          492 t
ORIGIN      
        1 actggcgcaa gggattggct gtcaccgctg aagccttcaa aggcaaagag attcgcgggc
       61 gcgctgtttt ggtccacaca ggttgggcgg aacattgggc aaccgatgcg tattttgaaa
      121 accatccctt tttgaccgaa gatgcagcga tttatctacg cgataatggc gcaaaacttg
      181 tcggtgtcga ctcgcataat attgatgaca cgcgtggcaa ttcgagacca gttcattcgg
      241 tgctgcttaa gaacgaaatc atcgttgccg agcatttatg caatcttgaa aaggtccctg
      301 acgagagatt caccttcaca gctgccccgc cgaaggtcaa aggtgccggg acttttcccg
      361 tccgcgcatt tgcgaaggta aattaagagc ttaagataaa agacttccga tggcatgcca
      421 tcggaagtct tttatcttgt aaggaaatta gtctattccg aaaggtcaat cacatccccg
      481 tcatattcac cagtgacgtg gattgcatac tctgcatcgc ggatgtaggt gagggtgcca
      541 tctttcagcc cggtgacgga gacttcgagg gtgaagctgc cgtcgtcgta gttgtaggag
      601 ccggtgcctg tcatggtgaa tccagcggag aaggcacaat ttgttagggt gtaggtgtcg
      661 ctggtatcgc ctgattcgaa ggtgacgttt ccgccgtagg ggcaagctgc ggcgttaatc
      721 tcttctgaga tggagtaata atattcgggc aaataattga tttcgttatc aaccgcgacc
      781 aacgcctcaa gtggatttgc atactcggaa gcatctgcca gagcgagcgg aacgaaatcg
      841 tctgccacgt aaccttcgca ggtggtttcg cgctcgggca ttttatcgtc tacgagaaag
      901 tcggtgacaa gcccatccac gcattcattg ccccagccaa agatgacatg tggaccgcca
      961 atttctgtga ccatgtatcc gttgtccaaa tgcgaaaaga cgtttttggc accttcgtag
     1021 ggagttgcgg gatcggtggt ggcgtttagc accaaggtag gaacaccgct gcccgtgaag
     1081 ggactaggga gcatggcggg ttgagaatca tctttcgggt agggccagta gacgcagggt
     1141 agatccccgt aaaagacaga agagaagcgc ggaagggttg catccacact atcgccttcg
     1201 cgcaggaaaa gctccgcttg ttcatcgaag gtggagccgg gctcggcata gtctcggcag
     1261 gtgacgccgt aatagatggc atcgctccag gagggatcta ataccgcgtc aagcgtatct
     1321 ggattgatat aaagcgagtt atagaccacg cgtgccaatg ggacgagggt gtcgtcacgg
     1381 gcgtaggctg ccaacgcccg caagaacatc atgcgcgcgg tttcagaata gagaaagcca
     1441 gaggtagctt caaggtctga gaagctgaac tcacggggtt gaacttcacc agtggaaagc
     1501 ggaaaatcaa acttaataga ttcttgtttt aattgcgcgg caagttcatc gtacacagca
     1561 acggcatctc tgcccatgtc agcggagcac gcttcatctg cattacaagc ttctagggtg
     1621 gcgaccatca catcgttaaa cgcggcggct tgacctttga gaaattcggt atcggtcaag
     1681 gtgatatcca ccgtgccatc gaggatgaga cccgccaaat ggtcggggtg agccgtggca
     1741 tacatctgtg agaattgcgt gccgtagctt tcgccgtaca gccagaattt atcatcgccc
     1801 attaacccgc ggaacacttc caagtcttct acggcttgtg tggtgtcgag gtaaggtaat
     1861 aggtcggtct gaccgacttc ggcgatgcat tcatctacaa atgttttggt cacgtcgagt
     1921 agttgagctt ctccttcggg cgtggtggca tcggtattcg tgctgtaata ttttgtgacc
     1981 gcattgacac aatacaaacc gcccgattgg ttgatgccgc gttggtcgaa gaagacaata
     2041 tcaaaatgtt cggtgaggcg cgg
//
LOCUS       DAOREF010000616         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43292_length_2062_cov_2.777280, whole genome shotgun sequence.
ACCESSION   DAOREF010000616 DAOREF010000000
VERSION     DAOREF010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43292_length_2062_cov_2.777280"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PKI33_12095"
     CDS             complement(<1..926)
                     /locus_tag="PKI33_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNM37801.1"
                     /translation="MKNEALRAVGYRRVSMREQVDGFSLDAQENNIRQYVEAQGWQLT
                     HIYTDAGISAKKDSHRPSLEQMMLDAEARRFDVIVVDKVDRFYRHLRGLLSALDQLNS
                     WNVAFASVQERLDFTTVWGKLTLTVLGTLAEIYIDNLRQETKKGKLQRARDGYWNGNP
                     TYGYCRGLCSACKEPNGKGYCPDYGLANKGDGKGLVLHPIESKVVRLVYDWYLTGEES
                     DGHIAERMRTYELDEHGLLARSRGIPGQKGPGPIQKDSIRNILTNILYAGFIPYYGTS
                     KMGRTTTRRVKEIFPGKHPAIVSMEEYERVQE"
     gene            1011..1433
                     /locus_tag="PKI33_12100"
     CDS             1011..1433
                     /locus_tag="PKI33_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNM37802.1"
                     /translation="MADASLAKKRKLSVKKKYDPKPFIDRIEQLMEARNLSMRETALS
                     SGLDHQAIRRIKAGQRRPDMTYCILLANFFDLNPNELLQLGGWPTLKVFDIKQVSAKR
                     LPPEAVDVALDIARIPDPGTRKQVAEAVRVLLKKYFGD"
BASE COUNT          524 a          515 c          419 g          604 t
ORIGIN      
        1 atctcttgta cacgttcgta ttcttccatg ctaactattg cgggatgctt tccgggaaat
       61 atctctttca ctcttcgtgt tgtcgttcgt cccatttttg acgtaccgta gtacgggata
      121 aaacccgcgt agagaatatt cgtgagaatg ttacgaatcg agtccttctg gattggtcct
      181 ggtcccttct gaccaggaat gccgcgagac cgggcaagca aaccatgttc atccaattca
      241 taagttctca ttcgttctgc gatatgcccg tccgactcct cccctgttag ataccaatca
      301 tatacaagcc gaacgacctt gctctcgatc gggtgcaaca ccagtccctt tccatctccc
      361 ttattagcca atccataatc tggacaatat ccttttccat tgggctcttt acaggcagaa
      421 cacaacccac ggcaataacc atacgtggga ttcccattcc aatacccatc gcgggcacgt
      481 tgcaacttcc ccttcttcgt ttcctgacgc agattatcta tatagatctc tgccaaggtg
      541 cctaacaccg tcaatgtcag ttttccccag acagtagtga aatccaaacg ctcctggacg
      601 gatgcaaaag cgacgttcca actattaagc tgatcaagcg cactcagtaa tccacgcaga
      661 tgacggtaga aacggtccac cttatcaaca acaatgacat cgaaacgtct tgcctcagca
      721 tctaacatca tttgttccag tgatgggcgg tggctgtctt tttttgcgga gatgcctgcg
      781 tcagtatata tatgtgttaa ctgccagcct tgcgcttcca catattgccg gatgttgttt
      841 tcctgcgcat ccagactgaa gccatccacc tgttcccgca tggacacacg ccgatatccc
      901 acagctcgaa gtgcttcgtt cttcatctta ccacctccta ttgggtataa tttcctatcg
      961 agaccgctgg gtctgcattc attttatctt taacacctgg agcacttcgt atggctgatg
     1021 cgtcacttgc gaaaaaaaga aagctttctg tcaaaaaaaa gtacgatccg aaacccttca
     1081 tcgatcggat cgagcaatta atggaagcaa gaaacctcag tatgcgcgag actgcattaa
     1141 gcagcgggtt ggatcatcag gcgatccgca ggatcaaggc ggggcagaga cgtccggata
     1201 tgacgtattg catcctactg gcaaattttt ttgatctcaa tccaaatgag ttattacaac
     1261 ttgggggctg gccaaccttg aaggtttttg atatcaaaca agtgagcgct aaacgtctcc
     1321 cccccgaggc agttgatgtt gcattggata ttgccaggat tcccgaccca ggcacccgca
     1381 aacaagtcgc agaagcggtg cgggtattac taaaaaaata ttttggtgat taacgaaaaa
     1441 gaacagactg tgcaagcctg ttcttttttt ctatttatct actatcaagc cacccgatgg
     1501 caagttaagt aaacgatcaa taataggtag ggattgcttt agtttgtcct tgcgtcctag
     1561 tcaggaacct gccagtgttg ggatcacacc tgcactgcac cgagtgcaac gagatgcaag
     1621 tgtaatactg gtactctgtt ggggctttta ctaaatctca gccgcacttt ttcacgcagt
     1681 aagcgattta cacattccca aatacagtcg tatcaccaca gacgaaaaca atggtataca
     1741 ccttttcaag gtggtatttc cttcagcaac gatctccatg cgcaaccgcc ttgtagtatg
     1801 taatcatatg ccagtcatgc tcacactgat aatctttttt gcttcggctg gcagatatgg
     1861 tcaaattttc gagcattgtg tatcattcag ttcagttgtg tacgtatgtt gtgatttggt
     1921 catccaataa ttttgcccaa ccatccctat ttttcaaggt ttttaggctc tctcttaata
     1981 tttacgtatg aatttcgaac agttaattac tttaggtttt tcattttttc tattcagttg
     2041 ttcttccttg gaaaggtctg ta
//
LOCUS       DAOREF010000617         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43359_length_2060_cov_2.876808, whole genome shotgun sequence.
ACCESSION   DAOREF010000617 DAOREF010000000
VERSION     DAOREF010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43359_length_2060_cov_2.876808"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PKI33_12105"
     CDS             <1..1245
                     /locus_tag="PKI33_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNM37803.1"
                     /translation="VKMKAGRDHWWHELVDVQNDVCNTEQTSAEDPLMIIYTSGTTGK
                     PKGALHTHCGFPVKAAQDMAFGTDVHQNDVIYWMTDMGWMMGPWLVFGSLILGATIFL
                     YDGAPDFPTPSRLWELVERHKINQMGVSPTLIRSLIPHGDEYFKKYDLSSLKCFASTG
                     EPWNPDPWMWLFANVGEKRIPIINYSGGTEISGGIVMGNMLLPLKPCAFSAPCPGMDA
                     DVVDENGKSVRNAVGELVIKAPWIGMTRGFWKDQQRYLDTYWSRWENVWVHGDFAAID
                     NDGLWYILGRSDDTIKIAGKRLGPAEVESILVRHEAIVEAAAIGVPHEVKGSELVLFA
                     VTKPGVERSAVLRQELQEMVVAEMGKPLAPKAILFVNDLPKTRNAKVMRRMIRAAYLG
                     LELGDTSSLVNPQAVEEIRKSL"
     gene            1399..>2060
                     /locus_tag="PKI33_12110"
     CDS             1399..>2060
                     /locus_tag="PKI33_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37804.1"
                     /translation="MRNNFRGLLFVFLSLVLMMMACNLGASAPTEVVSEPPAEALSAA
                     TDAPVTAEVQHVMAPGELPEKQSGLAGDQDSSTTADEKRAPAGDRFTYGRYERPFNAN
                     EMDVYFSNIDILVSEVYQDDVWIYGTLTLKDTGSGCSLDGKYGFEIDTNIDGGGDLLV
                     LVTKPTSTDWSTDGVEVWFDENDDVGGTKKVFSDESSSVGNGYESQLFGAGAGNDPDL
                     AWA"
BASE COUNT          522 a          391 c          588 g          559 t
ORIGIN      
        1 gtgaagatga aagcaggaag agatcattgg tggcatgagt tggtcgatgt gcaaaacgat
       61 gtttgcaata ctgaacaaac ctctgctgaa gacccgttga tgattattta tacctctggc
      121 actactggca aaccgaaagg tgccttgcat acccattgtg gattcccagt taaggcagca
      181 caggatatgg cgtttggcac ggatgttcat caaaatgatg tcatttactg gatgacagac
      241 atgggttgga tgatggggcc atggctggta tttgggagtt tgattcttgg cgcgacgata
      301 tttctatatg atggtgcgcc agatttccca acaccgagcc gactttggga gttggtagaa
      361 agacacaaaa tcaatcaaat gggcgtttcg ccaacgttga tccgctcgtt gattccgcat
      421 ggggacgagt attttaagaa atatgatctg tcttcgctca aatgcttcgc ctcaactgga
      481 gagccgtgga atccggaccc gtggatgtgg ttgtttgcaa atgtgggcga gaagagaatt
      541 ccaatcatta actattctgg cgggacggaa atctctggcg gcattgtaat gggcaatatg
      601 ttgttgccac tcaaaccttg tgcattttca gctccctgcc cgggcatgga tgcagatgtt
      661 gtggatgaga acggtaagtc ggttcggaat gcggtgggcg agttggtaat aaaagccccg
      721 tggattggga tgacgcgcgg cttttggaaa gatcaacaac gctatttgga tacgtactgg
      781 agccgatggg agaatgtttg ggtacatggc gactttgcgg caattgataa tgacggcttg
      841 tggtacatcc ttgggaggtc agacgacacg atcaaaattg ccggcaagcg gctcggacct
      901 gctgaggtgg agtcgattct tgtgcggcac gaggcgattg tagaggcggc ggcaattggg
      961 gtgccacacg aggtcaaggg gagtgaactg gtccttttcg cagtgaccaa gcccggggta
     1021 gagcgaagcg cggttcttcg tcaggaattg caagaaatgg tagttgcaga gatgggtaaa
     1081 ccgcttgccc cgaaagctat cctgtttgtg aatgatttac ccaaaacgcg caatgctaag
     1141 gtaatgcgcc gaatgattcg cgcggcatat ttggggttgg agttggggga tacttcttcg
     1201 ttagttaatc cgcaggcggt ggaagagatc cgaaaatctt tatagaaaac tcaatgcatt
     1261 tcaaaaggcg aggataacat cctcgccttt tatattttaa tgacataagt actagctttg
     1321 cttttaggtg agactatttt cctattgttt tacaccatta tcaaactgta tagtcttggt
     1381 aagaaaggaa aggtttttat gagaaataac tttagaggtt tattgttcgt gtttttgtct
     1441 ttggtgctta tgatgatggc atgtaattta ggtgcctctg cacccactga agttgtaagt
     1501 gagccgcctg cagaagcttt atctgcggca acagatgcgc ctgttacggc agaagttcag
     1561 catgtgatgg cgcccggtga attgccagag aaacaaagtg gtttggcagg tgatcaagac
     1621 tcatccacaa ctgcagatga aaagcgcgcc ccggctggag atcgcttcac ctatgggcgt
     1681 tatgagcgtc cattcaatgc caatgaaatg gatgtgtatt tttctaacat tgacattctc
     1741 gtgagcgagg tctatcagga tgatgtgtgg atatacggaa ctctgactct caaagatacc
     1801 ggttcaggct gttccttaga cggcaagtat gggtttgaaa ttgataccaa tatcgatggc
     1861 ggcggtgatt tgcttgtgtt ggttaccaaa cctacatcta ctgattggag cacggatggt
     1921 gtagaggtct ggtttgatga gaatgatgat gttggcggca ccaaaaaagt attttctgat
     1981 gaaagctcat cagtaggcaa tggatatgaa agtcagttat tcggtgccgg agcggggaat
     2041 gacccagacc ttgcttgggc
//
LOCUS       DAOREF010000618         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43390_length_2059_cov_4.630739, whole genome shotgun sequence.
ACCESSION   DAOREF010000618 DAOREF010000000
VERSION     DAOREF010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43390_length_2059_cov_4.630739"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /gene="dnaB"
                     /locus_tag="PKI33_12115"
     CDS             complement(<1..1225)
                     /gene="dnaB"
                     /locus_tag="PKI33_12115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNM37805.1"
                     /translation="MTDFYPEETTAPSSPGVPHSREAEEAVVGAVLINPEVYYDLAQF
                     LSADDFYIHRNRWIWDAFIRLHEARTPVDLLTLSEDLEKAGQLAEIGGSAYLTSLINQ
                     VPTSLNAEAYGHIVEENSLRRRMIQAANQIASLAYKGDGIDNVMGEAEKAVFGVSERR
                     TRHDVEPIRKVLSDYYDQVAELAKLGADFHGVPTGFIDLDKLLRGLHPSDLLIIAGRP
                     GQGKTGFLLSIAKNAGLTYKKHVAIFSLEMSNEQVVQRLIAQETGISSQNLATGKLAD
                     DEWKLFNHAIEVFGSTKIFLDDTPALTPLQLRTKCRRLHAEHKLDLVIIDYLQLMTGD
                     TRNDNRVQEVSNISRNLKVLARELNVPVLTAAQLSRAVEQRTDKRPILSDLRESGSLE
                     QDADIVMFIYRPDDYE"
     gene            complement(1241..1813)
                     /locus_tag="PKI33_12120"
     CDS             complement(1241..1813)
                     /locus_tag="PKI33_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNM37806.1"
                     /translation="MSSEMVEVVIDSIRVHLMAPQRVVVLKQVNAERYLTIWVGPYEA
                     EAITVALQEVEMVRPLTHDLLKNVFKAFNARIVRVEIVKLEADIYFGNIVAEADGREI
                     HIDSRSSDAIAIAARAHAPIMVHPSVMESAGVVPEQDMPEAPITPARKETPPPPLPDD
                     ASDRLSVFKDFIEKLDIDNPDKDKPESPST"
BASE COUNT          486 a          516 c          553 g          504 t
ORIGIN      
        1 tttcataatc atcggggcga tagatgaaca tgacgatgtc ggcatcctgt tccaacgagc
       61 cggattcgcg caagtctgag aggatgggac gtttgtcggt acgttgttca acggcacggc
      121 tcaactgcgc tgcagtcaac accggcacgt tcaactcacg cgccaacact ttgagattac
      181 gcgagatatt cgaaacttct tgcacacgat tgtcgttgcg agtatcacct gtcataagct
      241 gtagataatc aatgatgact aaatccaatt tatgctcggc atgcagacgt cgacacttcg
      301 tacgcaactg caacggagtc aatgcgggcg tatcgtccaa aaagattttg gtgctgccga
      361 agacttcaat ggcgtgattg aacaacttcc attcgtcgtc tgcaagttta ccggtggcta
      421 aattttgtga agagatcccc gtctcctgtg cgatgagacg ttgtaccact tgttcattgg
      481 acatttccaa agagaagatg gcaacgtgct ttttataggt caacccggca ttcttggcaa
      541 tggaaagcaa gaaacccgtc ttaccttgac ccggacgacc cgcgataatc aacaggtctg
      601 aaggatgcaa cccgcgcagc agtttatcga ggtcaatgaa accagtcggc acgccatgaa
      661 agtctgcgcc gagtttggca agctctgcca cctggtcgta ataatccgaa aggactttac
      721 gaatcggctc cacgtcatga cgggtgcggc gttcgctcac gccaaagaca gctttctctg
      781 cctcccccat tacattgtcg atgccatcgc ctttatacgc caacgaagcg atctgattcg
      841 cggcttggat catcctgcgc cgcagggagt tctcttcaac gatgtgacca tacgcctcag
      901 cgttgagcga ggttgggact tggttaatga gcgaggtcag gtaggctgag ccgccaatct
      961 ctgccagttg accggctttc tccaaatctt cagagagggt caacaagtct acgggggtgc
     1021 gggcttcatg caaacggatg aaggcatccc agatccagcg gttgcgatga atgtaaaaat
     1081 catccgccga gaggaactgg gcgaggtcgt aatacacttc ggggttaatc aacaccgccc
     1141 ctacgacggc ttcttcggct tcccggctat gcggtacacc tgggctggag ggggcggtag
     1201 tttcttcggg gtagaaatct gtcattgggt gagatcagct ttaggttgag ggtgattcgg
     1261 gcttgtcctt gtcggggtta tcgatatcca gtttttcgat aaagtctttg aagacggata
     1321 agcgatcact cgcgtcatcg ggcaagggtg gaggcggggt ttctttgcga gccggggtaa
     1381 tgggcgcttc gggcatgtcc tgttctggga cgacccccgc gctttccatc acactggggt
     1441 gaaccatgat gggcgcatgt gcacgcgcgg caatggcaat cgcatccgac gagcgcgagt
     1501 cgatatggat ctcgcgtcca tccgcttcgg cgacgatgtt gccaaaataa atatccgctt
     1561 caagtttgac gatctctacg cggacgatgc gcgcattgaa tgctttgaat acgttcttga
     1621 gcaggtcatg agtaagcgga cgcaccattt ccacttcttg caacgccaca gtgatggctt
     1681 ccgcttcata cggtcccacc caaatggtga gataccgttc agcattaact tgtttcagca
     1741 ccaccacgcg ctgaggcgcc attaaatgca cgcggatgct gtcgataact acttctacca
     1801 tttcagacga cataggggac tccaatgcgc ttattttagc ataaaggttg ggggcatgcc
     1861 tcagattaat ataagaggaa gatatgtttt gttttgcaag gtagaccaag gatttagacg
     1921 atagaagaac cgtttccatc gttagccata attcaagcct aaaattgggt ttgcatttat
     1981 cggcaaagac gactataatg cccctcgctc aattcggggc gtggcgcagc ccggctagcg
     2041 cacttgcatg gggtgcaag
//
LOCUS       DAOREF010000619         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43427_length_2058_cov_4.255617, whole genome shotgun sequence.
ACCESSION   DAOREF010000619 DAOREF010000000
VERSION     DAOREF010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43427_length_2058_cov_4.255617"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..546)
                     /locus_tag="PKI33_12125"
     CDS             complement(142..546)
                     /locus_tag="PKI33_12125"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNM37807.1"
                     /translation="MEELTLTTPALLFPTVSLLMLAYTNRFLTLATIIRNLNDRYHDS
                     QDKNLLAQIDNLRYRVYLIRNMQIFGVLSLLMCVVSMFALFFNWTDGGVWSFGIALVL
                     MIISLGISLRELQISVGALDLLLTNLEEHESK"
     gene            complement(579..1094)
                     /locus_tag="PKI33_12130"
     CDS             complement(579..1094)
                     /locus_tag="PKI33_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37808.1"
                     /translation="MRTLLPDEKLSTVFIYTPTMLVRGDLILRENIRASIWLRTQGVP
                     NYIHLYAAQVIQLAGSPPKNYTSQETFIPTAEVIGFHLAPPAQDPLDYDATEANRKME
                     SVHVLAGSFEMKAKLRISTSADFASGLDVMNTSWLSLYEAEITNPYLPQLKLTVPMLL
                     ARPSKLTISLL"
     gene            1170..>2058
                     /locus_tag="PKI33_12135"
     CDS             1170..>2058
                     /locus_tag="PKI33_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNM37809.1"
                     /translation="MTQDKLWNISPRISPEADSALAAFPPLLRQVLFNRGFADDPSAR
                     AYLRGEPNSNTDPFQITDMQVAIERIRYALANNEPIAIYGDYDVDGVTATALLVETLK
                     KLGADIRGYIPNRFDEGYGLNNNALDELKADGVKLVITVDCGIRSPAEAAHAQTIGLD
                     LIISDHHHPDDDNLPPALAVINPKRHGDIYPDKDLAGVGIAYKIAEALLTKTDQADVV
                     NGQILNSLLDLVALGTVADLAPLVGENRVLVKKGLKQMRQTTRQGLFSLAAVADISLA
                     KVNAGNIGFGLGPRLNAAGR"
BASE COUNT          562 a          555 c          463 g          478 t
ORIGIN      
        1 tcagaagaag gggtatgcgt ccctctggcg cgcgagccgc ccaatacaat ggcttttacg
       61 cctgaaacca ttttgatttc atcaaccatg gtttgaataa gtttatctgt atccataacc
      121 acacactttc agatgcgatt tttatttcga ctcgtgctct tccaaattcg tcagcaacaa
      181 atccaaagcg cctactgaaa tttgcagctc gcgcagggag attcccaaag agataatcat
      241 caacaccaaa gcgatgccaa aactccacac accaccatcc gtccaattaa agaacaaagc
      301 gaacatgctg acaacacaca tcaacaggct caaaacaccg aagatctgca tatttcgaat
      361 gagatacact cgataacgca ggttgtcgat ctgcgctaga aggttcttat cctggctatc
      421 gtgatagcgg tcattcaaat tacggatgat cgtggcaagg gtcaaaaaac gattcgtgta
      481 cgccaacatc agtagcgaca ccgtagggaa caaaagtgct ggggtggtaa gggtcagttc
      541 ttccatagta aatactcaag cggcatatgc cgcacattct ataacaaact aatcgtcagt
      601 ttactggggc gtgccaacaa cataggcact gtcaacttca attgcggcaa atacggattt
      661 gtgatctctg cttcatacag agaaagccag gatgtattca tcacatccaa accggaggca
      721 aaatcggcgg aggtagagat gcgcaacttg gctttcattt caaaagaacc agccaacacg
      781 tgcacagact ccattttacg attcgcttcc gtcgcgtcat aatccaaagg gtcttgcgca
      841 ggaggcgcaa gatgaaaacc gatcacctcc gcagtaggaa taaaagtttc ctggctggtg
      901 taattcttgg gcggggaacc tgccaattga atcacctgcg ccgcatacaa gtgaatgtaa
      961 tttggcacac cctgagtacg caaccaaata cttgcgcgga tattttctcg caagatcaaa
     1021 tctccacgca ccaacatggt aggcgtataa ataaaaacag ttgataattt ttcatcgggc
     1081 aataaagttc tcatgcgtcg ctcctcaaca gattatactc gcgcccttcc tcttgacttt
     1141 ggtctcgttt atactccgca cactttaaca tgacacaaga caaactttgg aacatctcgc
     1201 ctcgcatctc ccccgaagct gattcagctt tggcagcctt tcctccgctg ttacgtcagg
     1261 tgcttttcaa tcgcggcttt gccgatgacc ccagcgcacg tgcctatttg cgcggcgaac
     1321 ccaacagcaa taccgatcct tttcaaatca ccgatatgca agtcgcaata gagcgcatcc
     1381 gctatgcgct tgcaaataac gaacccattg ccatttatgg tgactacgat gttgacggag
     1441 tcactgctac cgcattattg gtggaaacgc tcaaaaaact tggcgcggat atacgtggct
     1501 acatacccaa ccgctttgat gaaggctatg ggctcaacaa caatgcattg gatgagttaa
     1561 aagcggatgg agtcaagctg gtcatcaccg tagactgcgg catccgttcg cccgccgaag
     1621 cggctcatgc gcagaccatt gggctggatc tcattatcag cgaccaccac caccccgacg
     1681 acgataacct gccgcccgcg cttgccgtca tcaacccgaa acgtcatggc gatatatatc
     1741 ccgataaaga cctcgccggc gtaggcattg catacaagat cgcagaagcc ttgctcacca
     1801 agacagacca agcggatgtg gtcaatgggc aaattctcaa ctccttgctc gatctcgttg
     1861 ctctcggcac cgtcgcagac ctcgctccgc tggttggtga aaatcgcgta ctcgtcaaaa
     1921 aagggctcaa gcaaatgcgt cagaccactc gccaggggtt gttctcgctg gcggctgtgg
     1981 cagatatctc gttagccaaa gtgaatgccg ggaatatcgg ctttgggcta ggtccacgcc
     2041 tgaatgcggc gggtcgct
//
LOCUS       DAOREF010000620         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43529_length_2055_cov_4.274500, whole genome shotgun sequence.
ACCESSION   DAOREF010000620 DAOREF010000000
VERSION     DAOREF010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43529_length_2055_cov_4.274500"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..287
                     /locus_tag="PKI33_12140"
     CDS             75..287
                     /locus_tag="PKI33_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNM37810.1"
                     /translation="MSERIVGTVKWFNATKGYGFIGRDGGEDVFVHFSAIQTDGYRKL
                     EAEQKVEFSIEEGPKGLQAANVVPLQ"
     gene            523..789
                     /locus_tag="PKI33_12145"
     CDS             523..789
                     /locus_tag="PKI33_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNM37811.1"
                     /translation="MSILNVFKTNASRHTTASSGYVVPDPYRCVQCGICSYNCPINID
                     VRAHAWRGEPVKHSECLTCGECVARCPRGVLRFERTDLFTEGAS"
     gene            786..>2055
                     /locus_tag="PKI33_12150"
     CDS             786..>2055
                     /locus_tag="PKI33_12150"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM37812.1"
                     /translation="MKYVVIGTGVAGISAVEAIRSVDRAGEIVMLGDDPHGFYSRPGL
                     AYILTGELQDESLFPKKREDYLPLKFKYKKACVMRIDRANKTLHLDDKTPLTYDKLLI
                     ATGASAIPLTVPGANLQGVMKLDHLEDARGILKHAKRGRTAIVVGGGITALELTEGLL
                     ARGVHVHYLLRGNRYWSSVLDEHESRIVEGRLQAEGVTLHFRSEMQEIIGKSGKVRGV
                     RLLDGRIMKCDMLAYAVGIKPRVELAKSADLALDRGILVNEYLQTNDPSIYAAGDVAQ
                     VYDPLTGRSVLDSLWSPAREQGDAAGLNMAGKSQAYVKMVPFNVTRLAGLTTTIIGAV
                     GHGRDEDIVGIARGDSETWRQLPDAIVAQGGFDINRVRLLVGEDYLIGAVVMGDQKLS
                     VPLQRMISEKINISSIRDTLLKKDTKVADVI"
BASE COUNT          505 a          446 c          564 g          540 t
ORIGIN      
        1 ccgcgtgccc gacccgcctt tgaaagcgtt taggtggacg ggcataaatt tttatacagc
       61 aggagtaaag aaagatgtca gaacgtattg ttggaaccgt taagtggttc aatgcgacca
      121 aaggctatgg cttcatcggt cgcgacggcg gcgaggatgt gttcgttcac ttcagcgcca
      181 ttcagacaga cggctatcgc aagttggaag ccgaacagaa agtggagttc tccatcgaag
      241 aaggtcccaa aggcttgcaa gctgccaatg tagtgcccct tcagtaagat aataaaatat
      301 ctttgcctaa cagacagggc tgtcgtgaga cagccctgtt ttttttaaat tttacgaaat
      361 aagactttta agagaagttt catttctttt caaactcatt tcatttgtac atctttatga
      421 cattcgacac tatttggatg ccctcaggca gatttagtat agatatagaa atgccccgta
      481 actttttcaa attctaagcg accctgtaaa tggaggatat tcttgtccat tcttaatgta
      541 tttaaaacca acgccagccg gcatacaact gcctcatctg gctatgtcgt cccagacccg
      601 tatcgctgtg tgcaatgcgg tatttgtagc tataactgtc ccatcaatat tgatgtccgc
      661 gcccatgcgt ggaggggaga gcctgtaaag cacagtgaat gcctcacttg tggcgagtgt
      721 gtggcgaggt gtccgcgcgg ggtgttacgc tttgaacgaa cagacttgtt taccgaaggt
      781 gcctcatgaa atatgtcgtt attggtaccg gcgtagcagg catttcggct gttgaagcca
      841 ttcgcagcgt ggatagagcc ggtgaaatcg tcatgcttgg tgatgacccg catgggtttt
      901 attctcgccc ggggctggca tacatcctca ccggcgaatt acaagatgag tcccttttcc
      961 ccaaaaagcg tgaagattat ctcccgttga aatttaagta taaaaaggca tgtgtcatgc
     1021 gaattgaccg tgcaaacaaa accctgcatt tggatgataa aacgcctctt acgtacgata
     1081 agctgctcat tgcaaccgga gcttctgcca tcccgttgac tgtgcccggt gcaaacttgc
     1141 aaggcgtgat gaagctcgac catcttgaag atgcgcgtgg aattttgaag catgccaaac
     1201 gtgggcggac cgccattgtg gtgggaggcg gtatcacagc gcttgagtta accgaaggtt
     1261 tactggcgcg cggagtgcat gtccattatc tgttgcgcgg caatcgttac tggagcagtg
     1321 tgctcgatga gcacgagtcg cgcattgttg agggacgttt acaagccgaa ggagtgactc
     1381 tgcattttcg ctctgaaatg caagagatca ttggcaaatc tgggaaagtg cgtggagtcc
     1441 gtttattgga tggacgcatc atgaaatgtg acatgcttgc ctatgccgta ggtattaagc
     1501 ctcgggtgga gctagccaag tctgcagacc ttgctttgga tcgcggaatt ttagtcaacg
     1561 aatatttgca aaccaatgac ccgagtattt atgcggcggg cgatgtagct caagtctacg
     1621 acccgttgac tgggcgttct gtgctggata gtttgtggag ccctgctcgt gagcaggggg
     1681 atgctgctgg gttgaatatg gcaggcaaga gccaggcgta tgtgaagatg gtgccgttca
     1741 atgtcacgcg ccttgccggg ctgacgacga ccattattgg ggcagtgggg catgggcgcg
     1801 acgaagatat tgtgggcatt gcccgtggcg atagcgagac gtggcgtcaa ctgccagatg
     1861 cgattgtggc acaaggcggt tttgacatca accgtgtccg cttgttggtt ggcgaagatt
     1921 acctaattgg cgcagtagtg atgggtgacc agaaactctc tgtgcctttg caaagaatga
     1981 tctcagaaaa aataaatatt tcttccattc gtgatacgtt gttgaaaaaa gatacaaagg
     2041 ttgccgatgt gatcg
//
LOCUS       DAOREF010000621         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43608_length_2053_cov_3.761261, whole genome shotgun sequence.
ACCESSION   DAOREF010000621 DAOREF010000000
VERSION     DAOREF010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43608_length_2053_cov_3.761261"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKI33_12155"
     CDS             <1..193
                     /locus_tag="PKI33_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37813.1"
                     /translation="GRTRLTLCGYSKTPMLAMDGTEADGIQEAARNAYHEANDEWASA
                     EYRMDVAAVLAGRALAALT"
     gene            197..463
                     /locus_tag="PKI33_12160"
     CDS             197..463
                     /locus_tag="PKI33_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37814.1"
                     /translation="MQTCPICNKEVQSSQRYPNYVCAECADLAVSKDGRKLIFYNQSI
                     SGGYEAKYADTSEEYNSHDCYINGIKCHANEHHFGGIVIIKVES"
     gene            463..738
                     /locus_tag="PKI33_12165"
     CDS             463..738
                     /locus_tag="PKI33_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37815.1"
                     /translation="MTKEKIKAFEEIASIFEISNESAKYFLNHVQKSFKVERPPHKLI
                     LEFVEKQNYEFKPTPYDVALAMKESGVWDYDLGSPPPALEDEEDLYQ"
     gene            856..1341
                     /locus_tag="PKI33_12170"
     CDS             856..1341
                     /locus_tag="PKI33_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNM37816.1"
                     /translation="MEFQSGDWVVHCAHGLGQITAIETRSFGEQTIPYYQVQIGDLTI
                     WVPTDEHLGKRLRKPASQSDFSNLLNMLTNPPETLPSDRRQRNQYLIELLKDGTAETL
                     CKVIRDLSAIRKHRTWSEYDRDILRRVQKSLIGEWSFIFSINPHDAENELQKLLAKHI
                     A"
     gene            1352..1576
                     /locus_tag="PKI33_12175"
     CDS             1352..1576
                     /locus_tag="PKI33_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37817.1"
                     /translation="MQSEEKTILQRVVENFARTGNATDEQVTVVSLPKGKSSTIENDR
                     SVMLKEYKVDDKLIWAGYSSRSNTVYLSLG"
     gene            complement(1573..>2053)
                     /locus_tag="PKI33_12180"
     CDS             complement(1573..>2053)
                     /locus_tag="PKI33_12180"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37818.1"
                     /translation="LDIETLEILEDLLLDYEGTLLLVSHDRAFLNNIVTSTLILDGSG
                     DVKEFIGGYEDWKKQLDEVSASKPTPKPAQTTQAESKSESKSTVRKLSYNEKRELEEL
                     PKRIEKLESEQHELNQKMEDPAFYQQEGSIITQAVERLEQVHKELAQAYERWGELDG"
BASE COUNT          592 a          489 c          461 g          511 t
ORIGIN      
        1 agggcgaacc cgccttaccc tgtgcggata cagcaagact cccatgctcg ccatggatgg
       61 cactgaagcg gatggcattc aagaagccgc acgcaacgcc tatcacgaag ccaacgacga
      121 atgggcttct gccgaatacc gcatggatgt tgcggcggtg ctggcagggc gtgcattggc
      181 agcacttacc tagtctatgc aaacctgtcc aatctgcaac aaagaagtgc aatcctcgca
      241 acgttacccc aattacgtct gcgccgagtg tgctgacctc gcagtatcaa aagatggcag
      301 aaaactaatc ttctacaacc agtcaatttc tggtggatat gaagccaaat acgctgatac
      361 cagcgaagaa tataacagcc acgattgtta tattaatggg attaagtgtc acgccaacga
      421 acatcacttt ggcggcattg tgatcataaa agtagaaagc taatgaccaa agaaaaaatt
      481 aaagcctttg aagaaatcgc gagtattttt gagatatcca acgaaagcgc aaagtatttt
      541 ctcaaccatg ttcagaaaag ctttaaagtt gaacgcccgc cccataaatt gatccttgaa
      601 tttgttgaaa aacaaaatta tgaattcaag ccaactccat acgatgttgc cttagccatg
      661 aaagaaagcg gcgtatggga ttacgacctc ggctctccac cgcctgcatt ggaagacgaa
      721 gaagatctct atcaataaaa gcagctagca tcggtgatat aatcatccct atataacaga
      781 ttgacagtgt ctaagcgcca gtccttttgt cggactggcg cttttctttt cccatcaagt
      841 acaaaggaga cttatatgga atttcaatca ggcgattggg tcgtccattg tgcgcatggc
      901 ttggggcaaa tcacagcgat tgaaacgcgt tcttttgggg agcaaaccat tccctattat
      961 caggttcaaa ttggcgatct cactatttgg gttcccacag atgagcattt aggcaaacgc
     1021 ttgcgcaaac ccgcaagcca gtcagatttt agtaatctgc taaacatgct aacaaatcca
     1081 ccagagacac tgcctagtga ccgccgtcaa cgcaaccagt atctcattga actgctcaaa
     1141 gacggtaccg ccgaaacctt atgcaaggtt attcgtgacc tatccgccat tcgtaagcat
     1201 cgcacgtgga gcgaatacga cagagatatt ctgcggcgcg tacaaaaatc gttgatcggt
     1261 gagtggagct ttattttttc aatcaacccg cacgacgcag aaaacgaact gcaaaaatta
     1321 ttagccaaac atatagcata aggaaaatac catgcaatca gaagagaaga ccatcctgca
     1381 gcgcgttgta gaaaacttcg cccgtactgg aaacgccacc gatgaacaag tgaccgtcgt
     1441 cagtttacct aaaggaaaat ccagcaccat tgaaaatgac cgttccgtga tgctaaaaga
     1501 atacaaggtc gacgataaat tgatatgggc aggatatagc agtcgcagca ataccgttta
     1561 tctaagtttg ggctaaccgt ccaactcgcc ccagcgctcg taggcttgtg caagttcttt
     1621 atggacctgc tccagccgct cgacggcttg ggtgatgatc gaaccttcct gttgataaaa
     1681 agcgggatct tccatttttt gattcagctc atgctgttca gattcaagtt tttcaattcg
     1741 cttgggcaat tcttcaagct cacgtttctc gttgtaactt aacttgcgga cagtggactt
     1801 gctctcagac ttggattctg cttgggttgt ttgagcaggc ttcggtgtcg gttttgaagc
     1861 ggacacttcg tcgagttgct tcttccaatc ctcatatccg ccaatgaatt ctttcacatc
     1921 gccagagcca tccagaatca atgtgctggt gacgatgtta ttgagaaagg cacgatcgtg
     1981 gctgacgagc aaaagcgtgc cttcataatc gagcaagagg tcttcgagaa tttcaagcgt
     2041 ttcgatatcg agg
//
LOCUS       DAOREF010000622         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43640_length_2052_cov_4.849775, whole genome shotgun sequence.
ACCESSION   DAOREF010000622 DAOREF010000000
VERSION     DAOREF010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43640_length_2052_cov_4.849775"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKI33_12185"
     CDS             complement(<1..309)
                     /locus_tag="PKI33_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37819.1"
                     /translation="MNAARKSLILLVVLILSTLACGLENIQVVEAPNLEATITAQAAL
                     LQQGGQPAQDPAQQVIVVVTATPEGGVAPTEQVQAAQVLPPATGDVTVTVTTATNCRT
                     G"
     gene            complement(424..1239)
                     /locus_tag="PKI33_12190"
     CDS             complement(424..1239)
                     /locus_tag="PKI33_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37820.1"
                     /translation="MNTIRKSFSVIFSFLCIGLLIACIPYTPPPPPPPPGVALTLTVQ
                     AIQLGFFTQTAQALQSQPIVIVVTATPDPNNLAPTTDPNAAPAATTSSAVTITVSQNT
                     NCRLGPSQDFDEIGSLGPGQTAEVVGKDTYDNYWVIKLPNNPAITCWLWGQYATVTGD
                     ISTVADSATPTAISNGAPNAPSLLDAQVKCTKLSGKDYQYKITLKWADNSSDENKFVI
                     NTSTGKTFSTPPNKTSFSFEEGLPSETNLSATITAANEKGESQPVGTGAFKCP"
     gene            complement(1501..1863)
                     /locus_tag="PKI33_12195"
     CDS             complement(1501..1863)
                     /locus_tag="PKI33_12195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNM37821.1"
                     /translation="MTLDLAPFEKEEYCYLTTKGRKTGTPHEIEIWFTIHENAVYLLS
                     GGMDKSDWVKNLLQDPNVTLRIAGQTFSAFASILEDKELEPMIRMAMAIKYDEWEGQD
                     PSEWARTALVVKFIPQSL"
BASE COUNT          501 a          415 c          561 g          575 t
ORIGIN      
        1 accggtgcgg cagttggttg ccgtggtgac agtgacggtc acatcgccgg tggcgggggg
       61 cagcacctga gcggcttgaa cctgttcagt gggagcaact ccaccttcgg gtgtggcggt
      121 cacaaccacg atgacttgtt gagcaggatc ttgagcaggt tgcccgcctt gttgcaacaa
      181 ggcggcttgt gcggtgatgg tcgcctccag gtttggcgct tcaacgactt gaatattttc
      241 cagtccgcaa gccagcgtgg ataggatgag gacaactagc aagataagag attttcgggc
      301 ggcgttcatt cttaattctc ctcgttcaga gataattgct gaatagattt tcaccacaga
      361 gaaaataaaa ccttctctgt ggtgaatttt cttttactta ggctagatag tagggatgcc
      421 cttttatggg catttgaagg caccagttcc taccggctga gattcgccct tttcattggc
      481 ggcagttatt gtggcagaaa ggtttgtctc ggaaggaaga ccttcttcaa aactgaaaga
      541 ggttttattg ggtggtgttg agaaggtttt cccggtggac gtattgatta cgaatttatt
      601 ttcgtcggaa gagttatcgg cccatttgag ggtgatctta tattgataat ctttaccgct
      661 cagtttggtg catttcactt gggcgtctaa aagagatggg gcattgggag caccattgga
      721 aatagcagta ggcgtggcac tatctgcaac ggttgagata tcacccgtca ctgtggcata
      781 ttgtccccat aaccagcatg tgatcgcagg gttgttggga agtttgatga cccagtagtt
      841 gtcgtatgta tccttaccga ctacttctgc ggtctgacca ggtcccaagg agccaatctc
      901 gtcaaagtct tggctcggac cgagacgaca gttggtgttc tggctgacgg tgatcgtcac
      961 cgcgctggat gtagtagccg ctggggctgc attagggtct gttgttggag caagattgtt
     1021 cgggtcaggc gtagccgtga caacaataac aatgggttga gattgtaatg cctgggcagt
     1081 ttgagtgaag aagcccagtt gaatggcttg cacggtgagc gtcaacgcaa ccccaggcgg
     1141 aggcggggga gggggagggg tatatggaat acaagcaata agtaaaccaa tacataaaaa
     1201 ggagaaaatt acagaaaatg acttacgaat agtgttcatg gagcagctcc tgtacgaaat
     1261 gggaattccc taaaatggag gtagttgtat atttgccaaa tagtcatccc tttcactccg
     1321 gcgttatgaa aggctttacc tgtgcggagc ttggcacatg gcaaagagca tgaaataaaa
     1381 ttggcaaaac taaatgtccc caattagata atttccccca ttttactgga cttttcattt
     1441 acgtaaggtg ttcttaagta ctgtaattta ctcgtaatat gttatttatc acaaatgact
     1501 ttataaactt tggggaatga acttcactac caatgctgtc cgtgcccatt cactagggtc
     1561 ttgcccttcc cactcatcat acttgatcgc cattgccatg cggatcatgg gttctagttc
     1621 tttgtcttcc aatatactgg cgaaggcaga gaaggtttga cccgcaatgc gaagagtgac
     1681 gttggggtct tgcagcaagt ttttgaccca atccgatttg tccatgccgc cagagagaag
     1741 gtagaccgcg ttctcatgga tggtgaacca gatttcgatc tcatgaggcg ttccggtttt
     1801 ccgtcccttt gtggtgaggt aacagtattc ttctttttca aatggagcaa ggtctagggt
     1861 catatatgtc tccaaaatca ttcagtaggt gggctaagtc taggtatcgt attgtgtaat
     1921 atcaaaaact tcgttggaga gaaaataggt ttgtgccggt aatagaacgt gcttgcgttg
     1981 ccctgtggcg tagacggtgg tgcagaagtt gcgtggtaag cgtttgatga gtgccgagtc
     2041 gtacaacaga at
//
LOCUS       DAOREF010000623         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43727_length_2050_cov_5.739850, whole genome shotgun sequence.
ACCESSION   DAOREF010000623 DAOREF010000000
VERSION     DAOREF010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43727_length_2050_cov_5.739850"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PKI33_12200"
     CDS             complement(<1..855)
                     /locus_tag="PKI33_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNM37822.1"
                     /translation="MKTEFVSKRAKIYADVPDEKWNDWRWQLSNRLNSVEDIEKVLPL
                     TDSERKALQTQGLFRVDVTPYFISLIDPNDPNDPTRLQIIPRAEEMQAFTAMMEDSLA
                     EDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRSRIVGDPGQTFKREEFEMQIEYL
                     KRTPQVRDVLLSGGDPLVLAPKVLEELLSRLREIPHIEIIRIGSRVPVFMPMRVTQEL
                     CDMLAKYHPLWMNIHVNHSNEISKELAEATDRLTRAGIPVGNQSVLLAGVNDNVHIQR
                     QLVQDLVRI"
     gene            1061..1957
                     /gene="speB"
                     /locus_tag="PKI33_12205"
     CDS             1061..1957
                     /gene="speB"
                     /locus_tag="PKI33_12205"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNM37823.1"
                     /translation="MTEEGTHGYWGDLHRPDLTPEQTDFSIIGIPFDGLASARKGAAL
                     APERLRYWSRHLTPFSEDRTRLKDMTICDLGDIEIKNPETDFVTVRQRVAALPNMPII
                     LGGDHSVTIPVLQAQRERYKDQRLGILWIDAHPDLCDFFDGSRLSHANTMRRALDFGI
                     EAHDVCMVGLRSWEEQEIDLIENGGIHVYTAADVAERGIRHIADSIYNILNDCDAVHV
                     SLDIDCLDPSVAPGTGIPEFGGLTSRDVLTLIKSTQDLSVVGLDVVEIAPSLDPSEAT
                     VFAGLKIIMEYIAVMARKKQKG"
BASE COUNT          531 a          488 c          526 g          505 t
ORIGIN      
        1 aatacggacg aggtcttgca ctagctgacg ttgaatatgg acgttatcat tcacgcctgc
       61 caataacaca gactgatttc caaccgggat acctgcacga gtaaggcgat cggttgcttc
      121 agcaagttct ttgctgatct cattcgagtg gttgacgtga atattcatcc acaaaggatg
      181 gtatttggcg agcatgtcgc acaattcttg tgtgacgcgc atgggcataa agaccggcac
      241 gcgtgaacca atgcggataa tctcaatatg cgggatctcg cgcaaacggg aaagtaattc
      301 ttcaagaact ttcggcgcga gcacgagcgg atcgccgcca gagagcagca cgtcacgaac
      361 ctgtggcgtg cgtttgagat actcaatctg catctcaaat tcttcgcgtt tgaaggtttg
      421 cccggggtca cccacaatgc gggagcgagt gcagtaacgg cagtacgagg cgcactgagt
      481 cgttacgagc atgagaactc tgtcaggata ccggtgaaca agccctggca cgggagagtg
      541 gcggtcttcc gcaagggaat cctccatcat ggcggtaaag gcttgcatct cctctgcacg
      601 tggaataatc tgtaaacgag tcgggtcatt cgggtcgttg gggtcgatca acgaaatgaa
      661 atagggagtc acatccacac ggaatagtcc ctgtgtttga agagcctttc gttcgctatc
      721 ggtaagcgga aggacttttt cgatatcctc aacagaattg aggcggttgg aaagctgcca
      781 gcgccagtcg ttccactttt cgtcgggaac atcggcgtaa atcttagcgc gtttggaaac
      841 aaactcggtt ttcatggttc ctccattagt tgtaaataga tcagaatatc aacattaaaa
      901 aaagccactc acattggagg gtcgtggtcg gggaggtaaa aacctgctat gcccataaag
      961 gtgacgttct tcggcataat tgcattttac gggcaaacga taggggggtc aatgcgggta
     1021 gttttcaaag gtaaaatagc caaatccaag gagcataacg atgaccgaag aaggtaccca
     1081 cggctactgg ggcgacctac accgcccaga cctcaccccc gaacagaccg atttcagcat
     1141 tattggaatt cccttcgatg gacttgccag cgctaggaaa ggagcggctc tcgctcccga
     1201 acgcctgcgt tactggtcca ggcatttgac tccctttagc gaagatcgca ctcgcctgaa
     1261 agatatgacg atctgcgatt tgggtgatat cgaaatcaaa aaccccgaaa ccgattttgt
     1321 caccgtgcgt caacgtgttg cggcattgcc aaacatgccc atcattctcg gcggtgacca
     1381 ttctgtgaca atccctgttt tacaagccca acgtgaacga tacaaagacc aacgcctcgg
     1441 cattttatgg atcgatgctc acccagacct atgcgatttc ttcgatgggt cacgtctttc
     1501 tcatgccaac accatgcgcc gtgccttaga ctttggcatt gaagcccatg atgtatgtat
     1561 ggtggggttg cgctcttggg aagaacaaga aatagactta atcgaaaatg gcgggattca
     1621 tgtgtataca gctgctgatg ttgccgagcg tggcattcgc catattgcgg atagtattta
     1681 caacatctta aatgactgtg atgccgtcca tgtttcactg gatattgact gcctcgaccc
     1741 atccgttgcg ccgggcacgg gcattcctga attcggcggg cttacttcac gcgatgttct
     1801 aacgttgatt aagtcgacgc aagatttatc ggttgtaggc ttggatgtag tggagatcgc
     1861 tccgtcactt gacccttctg aagcaactgt atttgccggg ttgaaaatta tcatggaata
     1921 catcgctgtc atggcacgta aaaaacagaa aggctaatac atggactctg aaaaattgga
     1981 agtcattcat aacccaacag aaagccgctt tgagattagg cttgatggtc aactcgccaa
     2041 attggattat
//
LOCUS       DAOREF010000624         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_43937_length_2045_cov_3.425628, whole genome shotgun sequence.
ACCESSION   DAOREF010000624 DAOREF010000000
VERSION     DAOREF010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43937_length_2045_cov_3.425628"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..279
                     /locus_tag="PKI33_12210"
     CDS             88..279
                     /locus_tag="PKI33_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37824.1"
                     /translation="MKQEVIIKGNANEDLTRLIEVAIKNQLEILEKDIRASKVRLSAF
                     EKKLGYLQKNLKPCWQAGS"
     gene            382..609
                     /locus_tag="PKI33_12215"
     CDS             382..609
                     /locus_tag="PKI33_12215"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNM37825.1"
                     /translation="MESIARYLMLGGIILFCIGGGVYLASRFGIPLGRLPGDIRIEGE
                     HGSFYFPVTSSILVSVLLTIVLNLITRFLNK"
     gene            631..1521
                     /locus_tag="PKI33_12220"
     CDS             631..1521
                     /locus_tag="PKI33_12220"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HNM37826.1"
                     /translation="MKSKWTPLIIGILFMFFCCIPLVIGAFFFQTRPPGALSDSGYYV
                     RLTKVYYYPGFGLSQAFVIQGADPFSFETIDSTYAKDKAHVYYDGVQVPDADPATFEL
                     LDSTYARDSRHVYLNGQIFSNDPANFENVSENIYRDSQYIYWSGESISEDPQNLVVLG
                     NFGFYTYLKDSQTVFVNGNSIYGANPNTFQVISEGYSRDSEHVFYFDELISTADAATF
                     EALEQQYARDAVSAYWMGKPIPNSDPASFYILNADFECTADANHAYYQDQLIEGFDPA
                     TIPTNAQVNSCDLTTVYFTP"
     gene            1518..>2045
                     /locus_tag="PKI33_12225"
     CDS             1518..>2045
                     /locus_tag="PKI33_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNM37827.1"
                     /translation="MKTNDFDYHLPESSIAQTPAEPRDSSRLLVLHRTTGDVEHRLFR
                     DVCLFLRPGDLLVLNQTRVIPARIFAHKETGGKVELLLLRRRDALTWEVLVGGKGLRI
                     GKIVQVENGPSAEIIELLEGSERLIKFSEPIEPYFSQVGNVPLPPYIHEKLNDPERYQ
                     TVFSREPGSAAAPTAG"
BASE COUNT          551 a          486 c          448 g          560 t
ORIGIN      
        1 gcgtgtggca accaaatctg catatgagca tttggggtat caatatacag aagaaggtca
       61 acccagattg ctataaggat catcgccatg aaacaagaag tcattatcaa aggcaatgca
      121 aatgaagatt tgacccgctt aatagaagtg gcaatcaaaa atcaattaga gatccttgaa
      181 aaagatatcc gcgcctccaa ggtccgcttg agcgcattcg aaaaaaagct ggggtatctt
      241 cagaaaaatt tgaaaccatg ctggcaagcg gggagttgaa tgaatgtttg gatacattgg
      301 attggaaaat ggaattggca tctttccgct tattgaatca aaaatataag agcctgatcg
      361 aagcagaggt atcctaatca agtggaaagc attgcccgtt acctcatgct cggcggaatc
      421 attctcttct gcattggcgg aggggtctat ctagcgtccc ggtttggaat tccgcttggg
      481 cgcttgcccg gcgatatccg cattgaaggc gagcatggct cgttttattt tccagtgaca
      541 agttcgatct tggtttcagt actacttacc attgttttaa atctcattac ccgttttctg
      601 aataaataac tttatccata aggtgtacct atgaagtcaa aatggactcc gttgattatc
      661 ggtattcttt ttatgttttt ttgttgtatc ccacttgtaa ttggggcttt cttttttcaa
      721 acccgcccgc cgggagcctt aagcgatagc ggatattacg tccgcctgac aaaggtctat
      781 tactaccccg gctttgggct ttcgcaggca tttgtcattc aaggtgcaga cccattctct
      841 tttgaaacaa ttgattcgac ctatgcaaaa gataaggctc atgtgtatta cgatggcgtt
      901 caagtcccag atgcagaccc ggcaacattt gagctgctgg attcaaccta cgcaagagat
      961 tcccgccatg tctatttgaa cggtcagata tttagcaacg accctgcaaa tttcgaaaat
     1021 gtatcagaaa atatttatcg tgatagccag tacatttact ggagcggcga gtctatttct
     1081 gaagacccac agaatttggt agtgctcggc aactttggct tttataccta cctgaaagat
     1141 tcgcaaactg tttttgtcaa tggcaactcc atctatggcg caaacccaaa tacatttcaa
     1201 gtgattagtg agggatattc acgtgactcg gaacatgtat tttatttcga cgaactaatt
     1261 tcaacagccg atgccgccac atttgaagcg ctggaacaac aatatgcacg cgatgccgtt
     1321 agcgcctatt ggatgggcaa accaattccc aattcagacc ctgcatcttt ttatattctc
     1381 aatgcagatt ttgaatgtac cgccgatgca aaccacgcct attatcaaga ccaattgatc
     1441 gaaggctttg accctgccac cattccaacc aatgcgcaag tcaacagttg cgacctgact
     1501 actgtatact tcacgccatg aagaccaacg actttgacta ccatcttcct gaatcgtcca
     1561 ttgctcaaac gcctgccgag ccgcgtgatt catctcgact ccttgttcta catcgcacca
     1621 caggggatgt agaacaccgc ctctttcgtg acgtatgcct gtttctacgc ccaggtgatt
     1681 tactcgtcct gaaccagacg cgcgtaattc ctgcccgcat ttttgcccat aaagagaccg
     1741 gcggcaaagt ggagttgctc cttctccgcc gccgagatgc gctgacttgg gaagttttag
     1801 tgggaggcaa gggtttgcgc ataggcaaaa ttgtgcaagt agagaatgga ccaagtgctg
     1861 agattatcga attgctcgaa ggttctgaac gcctcatcaa attttctgaa cccattgaac
     1921 cgtatttttc acaggtgggg aatgtgcccc tcccaccgta catccacgaa aaactaaatg
     1981 acccagaacg ctatcaaact gtcttttcga gagagccggg gtctgctgcc gctcccacag
     2041 caggg
//
LOCUS       DAOREF010000625         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44013_length_2043_cov_3.694668, whole genome shotgun sequence.
ACCESSION   DAOREF010000625 DAOREF010000000
VERSION     DAOREF010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44013_length_2043_cov_3.694668"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PKI33_12230"
     CDS             <1..725
                     /locus_tag="PKI33_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM37828.1"
                     /translation="EQAILREGADTVAAFIAEPVMGVGGVIVPPDDYFPLIRQICDKY
                     EVLFIADEVITGFGRTGEWFALKHWNVKPDILSFAKAVTSGYAPLGGIQISDAIRDTM
                     ESAGDTEAYMHGYTYSGHAMACAVGLKNIEIMEQEEFPKRARELGKRLLDGLKKLEEF
                     SFVGDVRGLGLVCGVEIVSDKTTKAADPATTMKIFKAAEALGLRSRPLGNTLAFSPPL
                     SITEDEVDEIVKRLGAAMDGVA"
     gene            915..1283
                     /locus_tag="PKI33_12235"
     CDS             915..1283
                     /locus_tag="PKI33_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37829.1"
                     /translation="MTEGLIIDDNRQTADALQQMLGLLDLPARVAYGSSAAMSVLASV
                     VPQFICLDINMPGVDGTEVLAYIRREPRLMKVPVIIITSDDQPETRQHVMRGGAQAMI
                     IKPVTIEILEGAFKKAGIIE"
     gene            complement(1328..>2043)
                     /locus_tag="PKI33_12240"
     CDS             complement(1328..>2043)
                     /locus_tag="PKI33_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37830.1"
                     /translation="TACGAAATEPPPPTETSTPIIIYITATSQPTLTPTITFTPGPTN
                     TVEPSITPTPEFSFPTVTVNKQAHCRYGPSAAFLHAADLYAGDTGTVRNRAMYSNWLY
                     IKFDKLNYFCWVAPSVVDVVGDVTTLKRYEPNLQSIGSNQYGPPQNVQAMRNGDEVTI
                     TWDQMEMSDDKDRGYLIEAFVCQGGAYIWWTFSYPDQFSTSYTVKDEAGCASPSYGKI
                     YTVEKHGFSYPADIPWPAP"
BASE COUNT          481 a          460 c          563 g          539 t
ORIGIN      
        1 tggaacaggc cattctgcgc gaaggagccg ataccgtcgc cgctttcatc gccgagcctg
       61 tgatgggcgt cggtggcgtg atcgttccac ccgatgatta tttcccgttg atccggcaga
      121 tttgcgataa atatgaagtc ctctttattg cggatgaagt tattacgggc tttggtcgca
      181 ctggggagtg gtttgcgctt aagcattgga atgtaaagcc agatattctc tcttttgcca
      241 aagcggtcac gagcggatat gccccattag gcgggattca aatctccgat gcgattcgcg
      301 atactatgga gtctgctggc gatacagaag cctatatgca tgggtacacc tattctggtc
      361 atgccatggc gtgtgctgtt gggctcaaga acatcgaaat tatggagcag gaagaattcc
      421 caaagcgtgc tcgtgaattg ggtaaacgct tattagatgg gttgaagaag ctggaagagt
      481 tttctttcgt tggcgatgtg cgcggacttg gtttggtctg tggggtggag attgtttcag
      541 ataaaactac caaagctgcc gaccccgcta ccactatgaa aatcttcaaa gccgctgaag
      601 cccttgggct gcgttcccgt ccgcttggga atacgctggc gttttcgcct ccgctttcca
      661 tcaccgaaga tgaagtggat gagatcgtta aacgccttgg ggccgccatg gacggggttg
      721 cctaagctca gttgttaact atatcatttg agtttaatga cagtttctaa tatcccattg
      781 cgggctgtaa tcaggaagtg tatactcgcc ttacatcctc tccttggttg accggtgacg
      841 gggattaaac gagaggtatt tccaaacaaa aagaacaaga cgtgtctctt agagcgcgcc
      901 atattggagg actaatgacc gaaggattaa tcatcgacga taaccgccag acggcggacg
      961 ctttacagca aatgctcggg ttgttggact taccggcaag agttgcttat ggttccagtg
     1021 ctgccatgtc tgttttggca agcgtcgtgc cgcagtttat ttgtttggat atcaatatgc
     1081 ccggtgtgga tggtaccgag gtgttggcgt atattcgccg tgagccacgt ctgatgaaag
     1141 tgccggtcat tatcatcact tctgatgacc agcccgaaac gcgtcaacat gtgatgcgcg
     1201 gcggggcgca ggcaatgatc atcaagccag tgacgatcga aattctggaa ggcgccttca
     1261 aaaaagcggg catcatcgaa tagcaaaaat aaaggctcct gtttcaaaca ggagcctttt
     1321 gatttaatta aggtgcgggc caggggatat ctgccgggta agagaaccca tgtttttcta
     1381 cagtatatat tttcccgtaa gacggtgaag cacaaccggc ttcgtccttg actgtgtacg
     1441 aagtgctgaa ctggtctggg tacgagaaag tccaccagat gtaagcgccg ccttggcaaa
     1501 caaaggcttc aatcaagtaa ccgcggtctt tgtcatcact catttccatc tgatcccagg
     1561 tgatggtcac ttcatcgccg ttgcgcatgg cttgcacatt ttgcggcgga ccatattgat
     1621 tgctgccaat ggattgaaga ttcggctcgt agcgctttag tgtagtgaca tctccgacca
     1681 catccacgac agatggtgcc acccaacaaa agtaattaag cttgtcgaac ttgatgtaaa
     1741 gccaattgct atacatcgcc cggtttctca ctgttccagt atccccagca tataggtctg
     1801 ctgcgtggag aaatgccgca gacggaccgt agcggcaatg cgcttgcttg ttgaccgtga
     1861 ctgtgggaaa gctgaactca ggagtaggag taatgcttgg ttcaacagta ttggtcggac
     1921 caggtgtgaa ggtaatcgtt ggtgtcaatg tcggttgtga cgttgcagta atatagatga
     1981 tgatcggcgt tgaggtttct gtcggcgggg gaggttctgt tgcagccgca ccgcaagccg
     2041 tta
//
LOCUS       DAOREF010000626         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44151_length_2040_cov_4.422670, whole genome shotgun sequence.
ACCESSION   DAOREF010000626 DAOREF010000000
VERSION     DAOREF010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44151_length_2040_cov_4.422670"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKI33_12245"
     CDS             <1..311
                     /locus_tag="PKI33_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37831.1"
                     /translation="VAYGVYQSVDGQWQAEGPVRSGTLDEMLSQFESPTLNAGELTSE
                     ERKKVSKVKHLQLASPVLCVRRPAVLAELAWARWQDDDADDAVSLAPIYLHVAGTPIQ
                     "
     gene            317..775
                     /gene="rimI"
                     /locus_tag="PKI33_12250"
     CDS             317..775
                     /gene="rimI"
                     /locus_tag="PKI33_12250"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNM37832.1"
                     /translation="MAEVLIRKMIHADLEQVIEIDQISFSLPWPKRSFQYELDENFTS
                     RCWVADVDGRIAAMMVGWMIIDELHVATIATHPDYRGQGIGKKVLVHALLAARKEGVI
                     RAFLEVRQTNEAALNMYRSLGFVEDGRRKEYYKDNNEDAILMSLNDMTHL"
     gene            785..1429
                     /locus_tag="PKI33_12255"
     CDS             785..1429
                     /locus_tag="PKI33_12255"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNM37833.1"
                     /translation="MSAAETLERIAHEVSVCTKCALHESRKKAVTGEGPADAEIMFIG
                     EGPGFHENEQGRPFVGASGKFLDQLLEQAGLTRSDVFIANVVKCRPPANRDPQPEELM
                     ACGEYLDGQIEAINPSIIVTLGRISMGRYFPGAKITSVHGQMQNIDGRFIIPMYHPAA
                     ALHQPALRPSILADFAKLPDQLNEARAALGRKVVEKKKNVVVQQPEKPQQLSLF"
     gene            1448..>2040
                     /locus_tag="PKI33_12260"
     CDS             1448..>2040
                     /locus_tag="PKI33_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNM37834.1"
                     /translation="MSIKEDLIKKLKEKNAKVAILGLGYVGLPLAVVFGEAGFKVTGV
                     DPDKRKVEALNKGESYIPDVKTEAVAKLVKNGMLTATTDFSVLKEMDAVSICVPTPLR
                     QTGDPDMSFIVSAMEELSKYMHKSMVVVLESTTYPGTTRELLLPKLGTEHNLTVGEDW
                     FLAFSPERVDPGREDFTTINTPKVMGGITHACGEVATA"
BASE COUNT          539 a          402 c          577 g          522 t
ORIGIN      
        1 gagtcgctta cggggtgtat caaagtgtgg atggtcagtg gcaggctgag ggtccagtaa
       61 gaagcggaac actggacgag atgttgagtc agttcgagag tccgactttg aacgccggtg
      121 agttaacatc tgaagagaga aagaaagtct ctaaggtaaa acatttgcaa ttggcatctc
      181 ccgttttatg cgtgcgcaga ccggctgtat tggcagagtt ggcttgggcg cgctggcagg
      241 atgatgatgc cgatgatgcc gtgtcgcttg cgccgattta cttgcatgta gcaggcacac
      301 cgatccagta aagattatgg ctgaagttct aattcgtaag atgatccatg cagatttgga
      361 acaggtgatc gagatcgatc agatttcttt ttcactcccg tggccgaagc gttcctttca
      421 atacgaatta gatgaaaact ttacgtcgcg ttgttgggtg gctgacgtgg atggtcgtat
      481 tgctgcaatg atggtgggct ggatgatcat tgatgaattg catgtcgcaa caattgctac
      541 acatcccgac tatcgtggac agggaattgg aaagaaggtg cttgttcatg cccttttggc
      601 ggcgagaaaa gaaggggtga tccgcgcctt tcttgaagtg cgccaaacaa atgaagcggc
      661 tttgaacatg tatcgaagtt taggctttgt ggaagatggt cgaagaaaag agtattacaa
      721 agataataat gaggatgcca ttctgatgtc gttgaacgat atgacgcatc tgtaaggaga
      781 ctgcatgtct gccgctgaaa cccttgaacg aattgcccat gaagtttctg tttgtacgaa
      841 gtgtgctttg catgagtcgc gcaagaaagc agtgacaggt gaaggtccag cagatgcgga
      901 gatcatgttc attggggagg gtccgggctt tcatgagaat gaacagggac gtccgttcgt
      961 gggggcatcg ggtaagtttt tagatcaatt gttggaacaa gccggtttga ctcgttcgga
     1021 tgtgttcatt gcgaatgtgg tcaagtgccg tccgcctgcc aaccgtgacc cgcaacctga
     1081 agaattaatg gcatgtggtg aatatttgga tggtcagatc gaagcgataa acccgagtat
     1141 cattgtgacg ttggggcgta tttccatggg gcgatatttt cccggcgcaa agatcacttc
     1201 cgtacatggg cagatgcaaa atattgacgg aaggtttatc attcccatgt atcaccccgc
     1261 ggcagcgctt catcagccag ctttgcgtcc atccattttg gcagattttg caaagcttcc
     1321 ggatcaactt aacgaagccc gtgctgcatt gggaaggaaa gttgttgaaa agaaaaaaaa
     1381 tgtggttgtt cagcaacctg aaaagccaca acaattaagt ctattttgaa attgaaggta
     1441 aaagaaaatg tctatcaagg aagatttaat caagaaattg aaagaaaaga atgccaaggt
     1501 tgcgatcctt ggcttggggt atgtggggtt accgttggcg gtggtgtttg gtgaggcggg
     1561 attcaaagtc acgggtgtag acccagataa acgtaaagtc gaggcgctca ataaaggcga
     1621 gtcttatatc ccagatgtta agaccgaagc agttgcgaag cttgtcaaga atggtatgtt
     1681 aactgctaca accgattttt ctgtactgaa agaaatggac gccgtcagca tttgtgtgcc
     1741 aacgcctttg cgccaaaccg gcgacccaga tatgtctttc atcgtttcgg cgatggaaga
     1801 gttgagcaag tatatgcaca aaagcatggt ggttgtgttg gaatccacga cataccccgg
     1861 cacgactcgc gaattgctcc tgccgaaact tggtactgaa cataacctga ccgttggcga
     1921 agactggttc ctggcattct cgccagagcg tgtggaccct ggacgtgaag atttcacgac
     1981 catcaacaca ccgaaagtga tgggtggtat tacgcatgca tgtggcgaag ttgctacggc
//
LOCUS       DAOREF010000627         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44386_length_2034_cov_3.059121, whole genome shotgun sequence.
ACCESSION   DAOREF010000627 DAOREF010000000
VERSION     DAOREF010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44386_length_2034_cov_3.059121"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..671)
                     /locus_tag="PKI33_12265"
     CDS             complement(327..671)
                     /locus_tag="PKI33_12265"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNM37835.1"
                     /translation="MSRLSSPVQVPSGRQVQLHKAVQRYLNALPADASAKSVESFKVL
                     MDVGLTREFKQLRHLQGDLWELKVDAKSVKFMRFLFVARNGEFLFTHAFRKQTNQTPR
                     REIDLALRRANE"
     gene            complement(1058..1351)
                     /locus_tag="PKI33_12270"
     CDS             complement(1058..1351)
                     /locus_tag="PKI33_12270"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNM37836.1"
                     /translation="MFTSFDDYVKDKLQKDQEFAKEYERLELPSMLARQVIKLRVEQG
                     ITQAQLAKLVGTQQSSISRLESMTALPSLSFLQKIAEVLNARIEIRLVPNPKA"
     gene            1469..1681
                     /locus_tag="PKI33_12275"
     CDS             1469..1681
                     /locus_tag="PKI33_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37837.1"
                     /translation="MEYSIVKLVFRSFQRLFFVLFWYLVAGFSEAVVYMYVSVLIDVV
                     EQPEYFSVASCSEFPGFVLYMADVWV"
BASE COUNT          498 a          407 c          498 g          631 t
ORIGIN      
        1 gcgcagggag gaacgccgac gattgttcga gacccgactg ccattccaat gcggattggt
       61 gttgccggcg agaaaaccgc tggtgtacac gccaatggaa gatgggtctt ggcgggaaga
      121 ctacagcgga agggtgatct cacatgaacg gttgcaacaa tatctcgtaa ggtatggatt
      181 tatataaaca atatcctgct ccaagagcaa ccggattaat gcaaaacaag cgagaaacct
      241 cgcttgtttt gttttaacca tataccttca ctatcttcac agagaattag aacggtggga
      301 tgttgacaca tcccaccgga aattgcctac tcgtttgccc gacgcagtgc gaggtcgatt
      361 tcgcgacgcg gggtttggtt tgtctgcttt ctgaaggcgt gggtgaacag gaattcccca
      421 ttccgcgcta cgaacaagaa gcgcatgaat ttgacgcttt tcgcgtcaac cttgagctcc
      481 cacaagtcac cttgtaagtg acggagctgt ttgaactcac gggttaatcc tacatccatg
      541 agtactttga aggactcgac ggactttgcc gacgcatcag cggggagtgc attgaggtag
      601 cgttggaccg ctttgtgcag ttggacttgg cgcccgctgg gcacctggac gggggatgag
      661 agacgactca ctgggacctc cttttatttg ggtggacatg acaacacaaa tcttttcgaa
      721 agcgcaggta cataacgctt gatctaatag gcttgcggtc agcctcccat taagaacttt
      781 gcttcaaggg caacctcctt tctttgatct agatctcaaa aattcagcga atacccaaag
      841 tgcaaaatcg ttataaatag aggggagcta cagtctctct gtgtcagata tttggatgtg
      901 aaggtacaaa gtttccgctt tattgtgcca aaaacaggca gacggaataa gtgctatttt
      961 tcctgtacta cctgtttttt gtcacaacca aaccaaaaag ttattttctt ccaataacag
     1021 atctgatttg gtgttctgaa tatttagttg tcaaaaacta tgcttttggg ttggggacta
     1081 gcctgatttc tatccgagca tttaatactt ctgctatttt ctgcaggaag cttagtgatg
     1141 gtagagcggt catactttcg aggcgggaga tgctggattg ctgcgtgccg acgagttttg
     1201 ccaattgagc ttgcgtgatg ccttgctcca cccgcaattt gatgacctgg cgcgcgagca
     1261 tactgggcaa ttcgagcctt tcgtattctt tcgcgaactc ctgatctttc tggagtttat
     1321 cctttacata gtcatcgaag ctggtgaaca tgatatttaa tctccaaaat gttttatatg
     1381 aattatgttt tattatgatc ttttatgtaa ttaagtaatt ctattttcaa gaggtgggag
     1441 ggcgggtttt ctacccgccc ccttgttctt ggaatattcg attgttaagt tagtgtttcg
     1501 ttcgtttcag cgcctgtttt ttgtgctttt ttggtatttg gtcgctggtt tttctgaagc
     1561 tgtagtttat atgtatgttt ctgttctcat cgatgtagta gaacagccgg aatatttttc
     1621 tgttgcatct tgctcggagt tcccaggttt tgtcctttat atggcggacg tgtgggtatg
     1681 aaagttgggt tccgaacatt tcgaggagtt caatttgtct ggttactgag gctcgttcct
     1741 ttgcggataa ctggtccagg aactcttggg tggaagcgga tattattgcc ttgaaacttg
     1801 acgatatggg ttccattgac cctccatgta gaagaataac ttgtccataa gacttttctc
     1861 ctttctgtag tataggattg ttggtaaggt actttttcca actggcgaga taatatcaca
     1921 attttgtgat atttgtcaag agggattttt atgaatacat aggcaaaaac gctcagggag
     1981 aatggctgaa attgcatcga atgagagaaa atattatggg cagaatacac ttaa
//
LOCUS       DAOREF010000628         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44410_length_2033_cov_5.374115, whole genome shotgun sequence.
ACCESSION   DAOREF010000628 DAOREF010000000
VERSION     DAOREF010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44410_length_2033_cov_5.374115"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKI33_12280"
     CDS             complement(<1..850)
                     /locus_tag="PKI33_12280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM37838.1"
                     /translation="MTQTYDAIVIGAGVMGASLAFHFAERGLKVAILERKVTASGATG
                     HSSGLVRMHYDLAAESHLTFASYKNYFSIWKERVGGECGFQQTGFLQIAKREHEDKLR
                     GNVANQQKIGINTSVISVDEVRKLFPDLVTEYFDYAAYEPDSGYADATLTTNSFLDSA
                     KRNGATLIQNCEVTAIHVSGGKVTGVSTTQGDFSAPIVVNAAGPWAKHVAALAGIEVP
                     LVTWTHDVAFLHRPPTLGKFPAVIDDVINCYFRPEGSALILAAGEDESLRGEAPDAED
                     QTPTPTF"
     gene            complement(928..1353)
                     /locus_tag="PKI33_12285"
     CDS             complement(928..1353)
                     /locus_tag="PKI33_12285"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNM37839.1"
                     /translation="MSAKHNPFIKLFWSIHLKLYLWSSGRIGNTIRGLPVLLLTTKGK
                     KTGQLRTKALMYLPYGDAFVVIASNLGKDNHPAWWLNLQADSTATVQVKDARFTVRAR
                     EAEGDEREKLFQALAQKTSDYEQYRSWTSRRIPVVILER"
     gene            complement(1355..>2033)
                     /locus_tag="PKI33_12290"
     CDS             complement(1355..>2033)
                     /locus_tag="PKI33_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNM37840.1"
                     /translation="TCPAKNTVRWNEYVPQNGYALGTDIFDLTRSVGLRTVLVVGKEK
                     LRQITESTDFFGWVDNTDEEKDKTTLDNMALEQIDLGFGLMMVHFPDGDVVGHEYGWL
                     SKQQLSAYKKNDQSFGRFISAMKKNGYYDGTLFIITSDHGGALTDPPHGTNIPEAMTI
                     PWIISGPQIVPGELKTPVYIFDTAPTVAFALGLPLQAEWDGTPVYEAFGLPVDPLHQG
                     GCPGLTP"
BASE COUNT          465 a          489 c          557 g          522 t
ORIGIN      
        1 gaaacgtggg tgtgggtgtt tggtcttcgg catcaggcgc ttcgccacgc agtgattcat
       61 cttcaccagc ggcaaggatg agcgcgctgc cttccgggcg gaagtagcag ttgatgacgt
      121 catcgatcac agcggggaat tttcccaatg tgggcgggcg atgaagaaag gcgacatcgt
      181 gagtccatgt caccaatggg acttcgattc ctgccaacgc cgcaacatgt ttcgcccacg
      241 gtcccgccgc attgacgacg attggggctg agaagtcgcc ttgggtcgtg cttacgccgg
      301 tcacctttcc gccactgaca tggatggcgg tcacttcgca gttttgaatc agcgtggcgc
      361 cgtttcgttt tgcagaatca agaaaagagt tggtggtgag cgtcgcatcg gcatagccgg
      421 aatccggctc ataagcagca tagtcaaagt attctgtcac aaggtctgga aaaagtttgc
      481 gaacttcatc caccgaaata accgaggtgt tgatgccgat cttttgttgg ttcgccacat
      541 tgccacgcaa tttgtcttca tgctcgcgtt tggcaatttg caagaaaccg gtctgttgaa
      601 aaccacattc gccgcctacg cgctctttcc aaatgctgaa atagttttta tagctggcaa
      661 aggttaggtg tgactcagcg gcgagatcgt aatgcatgcg gacaagcccg ctggagtgcc
      721 cggtggcacc agaggctgtc actttgcgtt caagaatggc gactttcagt ccgcgctcgg
      781 cgaagtggaa agcgaggctg gcgcccatca caccggcacc gatcacaatg gcatcgtagg
      841 tttgagtcat agaagagctc cttttgccgc gaattgtgcg gttgagtttt tttggttgcg
      901 aggaatttta gcagagtggg gcgattgtta tcgttccaat atcaccaccg gaatacggcg
      961 ggacgtccat gaacgatatt gttcgtaatc agaagttttt tgtgccaatg cctgaaaaag
     1021 tttttcacgc tcgtcccctt cggcttcgcg agcacgaacc gtgaagcgtg catctttgac
     1081 ctgtactgtt gcggttgagt ctgcttgcag gttaagccac caagcggggt gattatcttt
     1141 cccaaggttt gaggcgatga caacaaaggc atctccatat ggcaaataca tcaaggcttt
     1201 tgtacggagt tgccccgttt tcttgccttt ggtggttaga agaagcacgg ggagtccgcg
     1261 aatggtattg ccaatgcgac ctgaactcca cagataaagc ttgaggtgaa tggaccaaaa
     1321 tagcttgata aaagggttat gtttggctga catactatgg ggttaagcct gggcaaccac
     1381 cctgatggag cgggtctacg ggtaagccaa acgcctcgta aacgggcgta ccgtcccatt
     1441 cggcttgcaa gggcaagccc agggcaaatg ccacggttgg agctgtatcg aaaatataga
     1501 ccggcgtttt taactcaccg gggacaattt gcggtccgct aatgatccag ggaatggtca
     1561 tggcttcggg aatatttgtg ccatgtggag ggtcggttaa ggcaccacca tggtcagatg
     1621 taatgatgaa cagcgtgccg tcataatatc cgttcttttt catggcactg ataaaacgcc
     1681 caaacgattg gtcgtttttc ttgtaagcgc tcaattgctg tttggaaagc cagccatact
     1741 catgcccgac cacatcccca tcggggaagt gcaccatcat cagcccaaaa cctaggtcaa
     1801 tctgttcgag cgccatgtta tctaaggtgg ttttatcttt ttcttcatcg gtgttatcca
     1861 cccagccgaa gaagtctgtg ctttctgtga tttggcgtag tttttccttg ccaaccacga
     1921 gcacagtccg caaaccgaca gaacgtgtga ggtcgaagat atcggtgccc aatgcatatc
     1981 cattttgtgg gacatattca ttccagcgca ctgtgttttt ggcagggcag gtg
//
LOCUS       DAOREF010000629         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44498_length_2031_cov_3.972166, whole genome shotgun sequence.
ACCESSION   DAOREF010000629 DAOREF010000000
VERSION     DAOREF010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44498_length_2031_cov_3.972166"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /gene="rimO"
                     /locus_tag="PKI33_12295"
     CDS             <1..1364
                     /gene="rimO"
                     /locus_tag="PKI33_12295"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNM37841.1"
                     /translation="GSNVSKNTFHLVSLGCSKNTVDSDSMAQLLTRDGYSFVEDPSKA
                     SVLIVNTCGFIGPAKDESYRVLGELAQGKKKNQVLIAAGCLTQRYGVEVAKKVPGIDG
                     IIGTRRWMDIVQVVEDARKGKHPEPIYHLPEAATVGADENGAMRVSVTGPSAYLKIAD
                     GCRRPCAFCAIPLIKGTAVSRPVESIIHEARTLRDSGVRELILIAQDSTDYGHDLGMK
                     DGLATMLEQLTDAVPDMDWIRIMYSYPGYVTDRMIEVMATRKQILPYLDIPLQHAHPK
                     TLYRMKRPSNMDWVHKTLAKMRATIKDLAIRTTFIVGYPGETDEEFQALYDFVEQTRF
                     DRAGAFQFSFEPGTTSAPLGDPVPAEVKQERYERLMELQQNISLQVNQSYVGKTLDVL
                     VEGFDNGISVGRSYRDAPEVDGYVVVEGKAKVGDIVPVRITGAFAYDLTGVLVEQQNL
                     IKL"
     gene            complement(1415..1921)
                     /locus_tag="PKI33_12300"
     CDS             complement(1415..1921)
                     /locus_tag="PKI33_12300"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNM37842.1"
                     /translation="MDTPSTPVLAFIYWMHMLATVTWIGSLAAINLLFLPASTRTLKL
                     PDQLSLITALQKRLEPLAWFCMGILVVTGLFQMSTSPHYDGFLSISTQWSLSILIKHG
                     FAVIMVVVSAIQTWEVLPAIQRTLLKKDKADEAELSRLQKRETLLLRLNLLLSALILG
                     ATAFARVS"
BASE COUNT          504 a          425 c          558 g          544 t
ORIGIN      
        1 caggatcaaa cgtgtctaaa aatacttttc atttagtttc tctcggttgt tcaaaaaata
       61 ctgtcgactc tgattcgatg gctcaattgc tgacccgcga tgggtatagt tttgtcgaag
      121 acccatcgaa ggcttcggtc ttgatcgtta atacctgcgg gtttattggt ccggcaaagg
      181 atgagtcata ccgtgtgctg ggggagttgg cacaaggcaa gaaaaagaat caagtgctta
      241 ttgcggcagg atgtttgacg caacgctatg gggtggaggt tgcgaagaaa gtgccaggca
      301 ttgacggtat tatcggcact cgccgttgga tggacattgt gcaggttgta gaagatgctc
      361 gtaagggcaa acaccctgaa ccaatttatc atttgccaga agcagctact gtaggagccg
      421 atgaaaatgg cgcgatgcgc gtcagtgtga cgggacccag tgcttatctg aaaattgcgg
      481 atggatgtcg ccgtccgtgt gctttttgtg ctattccgct cattaagggc acggcagttt
      541 cgcgccctgt tgagtccatt attcatgaag cgcgcacctt aagagatagt ggtgtgcgtg
      601 aactcatttt gatcgcgcaa gactcaactg attatggtca tgacctcggc atgaaagatg
      661 gtcttgccac tatgctcgaa cagttaaccg atgcggtgcc ggatatggat tggattcgta
      721 tcatgtattc ttacccgggt tatgtcaccg atcgaatgat tgaagtgatg gcaactcgta
      781 aacagatttt gccgtatttg gatattccgt tgcaacatgc tcaccctaaa acgttgtatc
      841 gtatgaagcg cccgtcgaat atggattggg ttcacaaaac gcttgccaaa atgcgcgcaa
      901 ccatcaaaga ccttgccatt cgtacgacct tcatcgttgg atatcccggt gagacagacg
      961 aagaatttca agccttgtat gattttgtgg aacaaacccg cttcgaccgt gccggagctt
     1021 ttcaattctc cttcgagccg ggtactacct ccgcgccttt aggtgaccca gtccctgctg
     1081 aagtgaagca ggagcgttat gagcggttga tggaattgca gcagaatatt tcgttgcaag
     1141 tcaatcaatc ttatgtaggc aaaacgcttg atgtgttggt ggaaggtttt gataacggca
     1201 tctctgtggg acgctcttac cgcgatgctc ccgaagtaga tggatatgta gttgtagaag
     1261 gcaaagcaaa ggtgggggat attgtccctg tacgaatcac tggtgccttt gcctatgatc
     1321 tgaccggcgt gctggtagaa cagcagaatt tgatcaagct gtaattgtta ttaaaataaa
     1381 aatcggaagt cgttacgact tccgattttt tgattcaaga tactcttgcg aatgcggttg
     1441 ctccgagaat aagagcggag agcaacaaat tgagccgtaa caaaagcgtt tcgcgttttt
     1501 gtagacggga aagttcggct tcatctgctt tgtctttctt tagtagagtc cgctgaatgg
     1561 cggggagcac ttcccaggtt tggatggcac tgacgacaac catgatgacg gcaaatccat
     1621 gcttgatgag aatagatagt gaccattgag tgctgattga aagaaagcca tcataatgtg
     1681 ggctggtgct catttggaat agaccggtca ccacaaggat gcccatacag aaccatgcaa
     1741 gaggttccag ccgcttttgc agggcggtaa taaggctaag ttggtcgggc agtttcaagg
     1801 tccgcgtgga ggcgggtagg aagagcaaat taatggctgc cagacttccg atccacgtga
     1861 cggttgccag catgtgcatc cagtagatga acgcgagaac tggtgttgag ggtgtatcca
     1921 tgtttatggc gtagctgtag gttcaacaac gggagcttct gttgctgcag gttcggcggg
     1981 gtaacatgcc gcatacgcct gtgctgagtt tgctgaggct tctccatcta a
//
LOCUS       DAOREF010000630         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44585_length_2029_cov_4.411854, whole genome shotgun sequence.
ACCESSION   DAOREF010000630 DAOREF010000000
VERSION     DAOREF010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44585_length_2029_cov_4.411854"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PKI33_12305"
     CDS             <1..691
                     /locus_tag="PKI33_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37843.1"
                     /translation="MHKLESIADQIRKNFDARTAARDQALAQARQLTRACSLAIRAVH
                     RDDTESMQAQLLEAHRLADTLRNSLVSYPDLFHAGYTQDALKEFVEANVTCALIKSQP
                     LPTPEELGVEPATYLNGLAEVVGELRRRTMDILRHGYSAEAERLMGVMDEIYSVLVTM
                     DYPDAITNGLRRQTDLARGIIEKTRGDITFSLRGGHLEKAIGRLLKQMNGEQAQGPEG
                     SEAFPPMDEDD"
     gene            694..1311
                     /locus_tag="PKI33_12310"
     CDS             694..1311
                     /locus_tag="PKI33_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37844.1"
                     /translation="MAAKKGGRRYPLVIFTHMMSRWWTAVFTLGLAMLGLAWAIRSWG
                     FEQWRWMTFAVLGGFTALAGILMWLFRKSAYVQPFADHLKLVTPFLRMNISYKRFRRT
                     STATMGGIFPPKSLPRSLQEIVEPIAKMSAIVIEVSALPMSQNALRLFLSPLFFKDKS
                     PHIIILVSDWMRFSAEMDSMRHGGADVPNVAHRRDISILSKLPKK"
     gene            1311..1721
                     /locus_tag="PKI33_12315"
     CDS             1311..1721
                     /locus_tag="PKI33_12315"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNM37845.1"
                     /translation="MNIYFACSITGGRELESFYQQFVAALEADGHVIPTSHLAQSEAM
                     EGERMLTPVDVYERDVKWVRECDVLIAEVGVPSHGVGYEIGYALNAGKPVLCLAQNGR
                     RVSKMITGNSALEMKTYSTLEEAITLARNFLSSI"
BASE COUNT          495 a          477 c          508 g          549 t
ORIGIN      
        1 catgcacaaa cttgaatcga tcgccgatca aattcgcaag aattttgatg ctcgcaccgc
       61 tgcacgtgac caagcattgg cacaggcgcg tcagttaacc cgcgcttgtt cacttgcgat
      121 ccgtgcggta caccgtgacg ataccgaaag catgcaagcc caacttctcg aagcccatcg
      181 tctggcagac acccttcgca attctctcgt ctcataccca gacctcttcc atgctggata
      241 tacccaagac gcgctcaaag aatttgtgga agccaatgta acctgtgcat tgatcaaaag
      301 ccagccgctt ccgactccgg aagaattggg tgttgaaccc gcaacctatc tcaacgggtt
      361 ggcagaagtg gtcggtgaat tgcgccgccg cacgatggat attctccgtc acgggtattc
      421 ggcagaagcc gaacgcttga tgggcgtgat ggacgaaatc tactctgtgc ttgtcaccat
      481 ggattatccc gacgccatca cgaacggact tcgccgtcag accgatcttg ctcgtggcat
      541 tatcgaaaag acgcgcggag atatcacatt cagtttgcgt ggcgggcatt tagaaaaagc
      601 cattgggcgt ttgctcaagc aaatgaatgg cgagcaggct caaggtccag aaggaagcga
      661 agcatttcct cccatggatg aggatgatta accatggcag caaaaaaagg cggacgccgc
      721 tatcctcttg tcatttttac acacatgatg agccgttggt ggacggcggt ctttactctt
      781 ggcttggcga tgcttgggct ggcgtgggca attcgtagtt ggggttttga gcaatggcgt
      841 tggatgacct ttgcggtgtt gggtggtttt actgcattgg caggaattct gatgtggctc
      901 tttcgcaaga gtgcgtatgt gcaaccattt gccgatcatc taaaactggt gacgccattt
      961 ttacgaatga atatttcgta taaaagattt cgccgtacta gcactgccac catgggcgga
     1021 atttttcctc cgaaaagctt gccacgttca ttacaagaga ttgttgagcc aatcgcaaag
     1081 atgagcgcca ttgtgatcga agtgagtgct ttgccaatgt cgcaaaatgc attgaggctt
     1141 ttcctttcgc cgcttttctt caaagataaa tctccgcaca ttattatcct tgtttcagat
     1201 tggatgcgtt tcagtgccga gatggatagc atgcggcatg gtggtgcaga cgtacccaat
     1261 gtggcgcatc gccgtgatat ttctattctt tcaaaactcc ctaagaaata atgaatattt
     1321 attttgcatg ttctatcacg ggtgggcgtg agttggaatc tttttatcaa caatttgttg
     1381 ccgcccttga agccgatggg catgtgatcc ctacttcgca tctggctcaa tctgaggcaa
     1441 tggaaggcga acgtatgctt actccggtag atgtgtatga acgcgatgtg aagtgggttc
     1501 gagaatgtga tgttttgatc gcagaagtgg gagtcccttc ccatggcgtg ggctatgaga
     1561 tcgggtatgc cttgaatgct ggcaaacctg tgctttgctt ggcacaaaat ggaaggcgtg
     1621 tttctaaaat gatcactggg aattctgccc ttgaaatgaa gacatattcc acccttgaag
     1681 aggcaattac tctggcacgt aatttcttga gcagcatctg atcttatgtg cttcatcatg
     1741 tgcaatgggt gacatattac gatgcccaaa aaacggaacc tgcattaata tcactcacgg
     1801 agacggttgt agacacctct caacgcagaa ttccagtctc ctcctttggc gaaagctgac
     1861 gtcaggtgaa cagcctgacg tcagcatttt aattcttgtt agcgcgcggg cacgtccaac
     1921 aactatagat tttatgtcta cagtttcacc ccactctcgg caacagatgt tgctcggatc
     1981 atagtcaaca acatcttgaa tttctggata gctttcaagg catgtggtt
//
LOCUS       DAOREF010000631         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44706_length_2026_cov_4.606799, whole genome shotgun sequence.
ACCESSION   DAOREF010000631 DAOREF010000000
VERSION     DAOREF010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44706_length_2026_cov_4.606799"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKI33_12320"
     CDS             complement(<1..251)
                     /locus_tag="PKI33_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNM37846.1"
                     /translation="MNDRAVTYARVSGDDRVKDGRNLSGQLDICREHALKKGYQIVAE
                     LSEDDRGASGVSFELPALNQALEMAKNGEFDILVVREIDR"
     gene            complement(334..573)
                     /locus_tag="PKI33_12325"
     CDS             complement(334..573)
                     /locus_tag="PKI33_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37847.1"
                     /translation="MLQTPSTQRFQIVGSLARIRQEWQDAAGTSSLIEVEGNMGMLLA
                     DLINGLDLGTNEQVQVLGQELFQELKDFLKSPVQN"
     gene            complement(837..1052)
                     /locus_tag="PKI33_12330"
     CDS             complement(837..1052)
                     /locus_tag="PKI33_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37848.1"
                     /translation="MEDWLTTYEAVQLSGYELDYIRKLVRAQKIVGRKWGQSWQVSRK
                     SLLDFLKSREKQGEKRGRKPKREQSQN"
     gene            1426..1623
                     /locus_tag="PKI33_12335"
     CDS             1426..1623
                     /locus_tag="PKI33_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37849.1"
                     /translation="MSNSSTGQPKQVFTPEQRLALGRVYSFLIEIGRQRLTRLQTTDT
                     SKPTIEDETPNNTTKTLINSE"
BASE COUNT          566 a          502 c          421 g          537 t
ORIGIN      
        1 cggtcgatct cacgaaccac caagatatcg aattcaccat tctttgccat ctccaatgcc
       61 tgattcaagg ctggaagttc aaatgaaacg ccagacgccc cacggtcatc ctcggataat
      121 tctgcgacta tttgataacc ttttttgagt gcatgttcac ggcaaatatc aagctgaccg
      181 gacaggtttc gaccatcctt aacgcgatca tctcctgaga cgcgcgcata tgtaactgct
      241 ctatcattca ttgtaagaaa cctctttttt gatcgttgta aaacaatatg gaagatacaa
      301 gccagccgcg ccggtaaggc tggcttgtat gcatcagttc tgaacagggg acttgagaaa
      361 atccttcaat tcctggaaca attcctgacc cagtacctgt acctgttcgt ttgttccaag
      421 gtcgagtcca ttgatcaagt cagcaagcaa cattcccatg ttgccctcca cctcgatcaa
      481 actggatgtc cctgcagcat cctgccattc ctggcggata cgggcgagag atccaacgat
      541 ctgaaagcgt tgtgtagaag gggtttgaag catatacatc tccttttgat tttgattggg
      601 cggtctgctt ctcccggcag aaacgcaaat cgccctgatg gaatcccacc aaggcgaatc
      661 aaaggggctg tataatcaaa ccagcccttc cctccgctct agccggtggg ttgggtgagg
      721 gtcgcgttgg tgctggaaca ccagtgcggc ccgtttttct ggctcttaat ttcattgcca
      781 gcaatgaaat tatagcacaa gttaaacgac tgacaagtcc ccgttacctt acatgatcaa
      841 ttttgggatt gttcccgttt gggttttcgt ccgcgcttct cgccttgctt ttcacggctc
      901 ttcaaaaagt caagcaagga cttacggctg acctgccagg attgtcccca cttgcgtcca
      961 acaatctttt gagcgcgcac caactttcgg atgtaatcca attcatatcc actcagttgc
     1021 acagcctcgt atgtcgtcag ccagtcttcc ataatatata gtagcgcctc caaacgatat
     1081 tataacgtcg tgaacggaga aatcgacact ggtgttccgg ttgtaaaagt accagacaga
     1141 ggtatagcat ccgggactcc gttaactcta cggattggtg tccgacttac gtatgacttc
     1201 gccccacaca taaaaaagaa ggttttggag ggaataaact cgtagggata tccgtattca
     1261 aatccgtagt agaaccgtac aggcaggtgc tatttttagg catgcagttg aatacttcaa
     1321 tttcttcagg aaggcagcca tgcataaaat agtacctatt ctcaaatatc gaaaaaataa
     1381 tttcaaattc actggacaag tatcctgcca ggaaagggga agatcatgag caattcttca
     1441 acaggacaac ccaagcaagt atttacacca gagcaaaggc ttgccttagg gagagtgtat
     1501 agttttctga ttgaaattgg acggcaacga ttaacccgtc ttcaaacaac agacacatca
     1561 aaacccacca tagaagatga aacgccaaac aacacaacca aaaccctaat caattctgaa
     1621 tgaggtcctt ccattgaaag aacacagcag gttgtgtaaa caagatttgg tctacacaac
     1681 ctgctgtact attctatgag tagttctcaa gcagattgat tttcaattat ttccacatcg
     1741 aaattgaaat gagttaaatc ccatcaaaat agtgccaaat atcagttgtc gaaaactccc
     1801 ctggttctct aaaatacccc gcccttactt taatatttga ggttacaaca atgaatcaca
     1861 tcaaaaggct ttggggacat taccttgcgt taatgatcct cgttgccgcc ctcgcgtatc
     1921 ccatctaccg ggtctaccga ttttttcaaa cccggtatgg acaggaagca gcactaatcg
     1981 cactcaccct gaccgtcatg gtcgttatgg ttttagtggt acgggg
//
LOCUS       DAOREF010000632         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44749_length_2025_cov_3.601523, whole genome shotgun sequence.
ACCESSION   DAOREF010000632 DAOREF010000000
VERSION     DAOREF010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44749_length_2025_cov_3.601523"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1191)
                     /gene="moaA"
                     /locus_tag="PKI33_12340"
     CDS             complement(187..1191)
                     /gene="moaA"
                     /locus_tag="PKI33_12340"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNM37850.1"
                     /translation="MTILDTLNRPLRDLRISVTDRCNFRCVYCMPKEIFGSDYKFLHH
                     SQVLTFEEITRIARLFAGLGVKKIRLTGGEPLVRKDFPDLVALLAQIPDLDLTMTTNG
                     SLLPRFAEQLKKSGLNRVTVSLDSLDNKIFKSMNDVDFPVEKVLEGMDAAKAAGLEPI
                     KVNMVVKRGVNEDSILPMARFFREKGFILRFIEYMDVGSTNGWRMNDVVSAKEIISII
                     NAEMPLEPADPNYRGEVAERWRYKDGQGEIGVIASVTQAFCRDCNRARLSAEGQLYTC
                     LFAVKGHDFRDMLRNGASDEEILHKIETVWGKRTDRYSEIRSDNTIPLPKVEMSHIGG
                     "
     gene            complement(1333..1611)
                     /locus_tag="PKI33_12345"
     CDS             complement(1333..1611)
                     /locus_tag="PKI33_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNM37851.1"
                     /translation="MKVNFFATLRDIAGGKTVEFDLDHGITARELLDAICERFPAMKK
                     ELLKEDGEMHGHVHFFINGRDVQFTDLQFATPITQGDTVNVFPAVGGG"
     gene            complement(1608..1940)
                     /locus_tag="PKI33_12350"
     CDS             complement(1608..1940)
                     /locus_tag="PKI33_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNM37852.1"
                     /translation="MQLRFYANMRTTVGMPLLEINEAMVDSFRKLIPLLLEQYPETAF
                     HLLDEHGELRPDVPVYVDGRNPRLSSVGMDTPLKPDSVISFFSPISSGKLNVEVLREP
                     TIDTRSEP"
BASE COUNT          563 a          488 c          449 g          525 t
ORIGIN      
        1 tcaggtctgc gaactcgacg acatgctcgt ggaatactac gagaagcgcg gatgggtgaa
       61 cggcgttgta cccgaatcca aactcaaaga actggaaatc atctagttca caagttgaat
      121 aaaaaagacc tcccgtattt tacgggaggt cttttttatg ttaggaacaa tagagaacgg
      181 aaatgcctaa ccgccaatat gagacatttc tacttttgga agcggaatgg tgttatcaga
      241 acgaatttca gaatatcggt ccgtgcgttt accccaaaca gtttcgatct tatgcaagat
      301 ctcttcgtca ctggcaccat tccgaagcat atcgcggaag tcatgccctt tgactgcgaa
      361 gagacaggta taaagttgcc cttctgcaga aagacgcgca cgattgcaat cacggcaaaa
      421 ggcttgtgtc accgaagcga tcacaccaat ttcaccctgc ccatctttat aacgccagcg
      481 ttctgccact tctccgcggt aattcgggtc agcaggttct agcggcattt cagcattgat
      541 gatgctaatg atctcttttg ccgaaaccac atcgttcata cgccagccat tggtactccc
      601 cacatccatg tattcaatga aacgcagaat aaaacctttt tcacggaaga agcgcgccat
      661 gggcaaaatg ctatcttcat tcacaccgcg cttcaccacc atattaacct tgatcggctc
      721 taacccagcg gctttagcgg catccatgcc ttcaagaact ttttccactg ggaagtccac
      781 atcgttcatc gacttgaata tcttgttatc caaagaatca aggctgaccg ttacccgatt
      841 caacccagac tttttgagtt gctcggcaaa ccgaggcaga agcgacccgt tggttgtcat
      901 cgtgagatca aggtcgggga tctgtgccag taatgccaca aggtcaggaa aatctttacg
      961 tacaaggggt tcgcccccag taaggcgaat cttcttgacc cccaagccag caaacaaacg
     1021 tgcaatgcgc gtaatctctt cgaaagtcag cacctggctg tgatgcagaa acttataatc
     1081 tgagccaaag atttccttcg gcatgcagta tacacagcga aaattacagc gatcagtgac
     1141 cgagatgcgc aagtcacgaa gaggacggtt gagggtatca agaattgtca taataggtga
     1201 gtgtcaacaa gctctgacat aaaggattat acccgtaagg atatgttgtt tttgacctaa
     1261 aaaccgcatc tttcttaatc tcaggtctga tacggcgctt ctaagctttt tcaatttcga
     1321 aatttgcctg aattaaccgc cgcccactgc ggggaagaca ttcacagtat cgccttgtgt
     1381 gatcggcgtg gcaaattgca ggtcagtgaa ttgcacatcc cgcccattga taaagaagtg
     1441 gacatgccca tgcatctcgc catcttcctt gagtaattct tttttcattg ctgggaagcg
     1501 ttcacaaatg gcatccaaca attcacgggc agtaatgcca tggtcaaggt caaactcaac
     1561 agttttacca ccagcaatat cacgtaaggt cgcaaagaaa tttactttca tggttcactc
     1621 cttgtgtcaa tagtcggctc acgtaacact tctacattta gttttccgct tgagatgggg
     1681 gagaaaaagc tgatgacaga atccggtttg agaggggtat ccattccaac cgaggaaaga
     1741 cgcgggtttc ttccatccac atagacggga acatccgggc gtaattcgcc atgttcatcc
     1801 agtaaatgga atgcagtttc gggatactgt tcgagtagca acggtattag tttgcgaaat
     1861 gaatcgacca tcgcttcgtt gatttcgagc agtggcattc caactgtggt tcgcatattg
     1921 gcataaaacc gtagctgcat attctgattt tatcattttc gttcgtgtta aaacaaagga
     1981 ctcggaagac ccgagtcctt tgaaaaccct agatcaaatt acggg
//
LOCUS       DAOREF010000633         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44950_length_2020_cov_4.574555, whole genome shotgun sequence.
ACCESSION   DAOREF010000633 DAOREF010000000
VERSION     DAOREF010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44950_length_2020_cov_4.574555"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKI33_12355"
     CDS             <1..345
                     /locus_tag="PKI33_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37853.1"
                     /translation="TLAHFQRAQPPFKEEADLILASFSEEAEPNPDEPESATNRKPSS
                     PEIVWEAVQDALFSEKPKLRYLVGTHWEGNRVLNALMSKLLDENDNPQHNYSRDELIA
                     LLDQHLTERNAS"
     gene            397..1131
                     /locus_tag="PKI33_12360"
     CDS             397..1131
                     /locus_tag="PKI33_12360"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNM37854.1"
                     /translation="MAAKELSHKPLKEEIYDALHRQIIAGRYGPGDWLRQEDIASQMG
                     VSMTPVREALDLLVAKGIAERVPYRGVRVREMSPEDVVEAYGMRLFLEAMVARETALN
                     ITPEQVQKLENIMAEMDRHVELNEMPQERQLSREFHSAIAEASGNKLLVELYSIVSNA
                     FPDWLLYEALYRQPELVSSSVSQTHDEHAAILNAFKKHDPDLAVKVSLEHVMESGRWL
                     ENYRNIPAKLLREKEKQVSHLIKKSK"
     gene            1149..1808
                     /locus_tag="PKI33_12365"
     CDS             1149..1808
                     /locus_tag="PKI33_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNM37855.1"
                     /translation="MSEEIEKLFKDMAQSIIDGDSDLSVELAEKSIAMNIPPLDAITK
                     GFVIGVNYIGDQFGAGEAFLPELVMAGEAMKAAVATLEPELLKLGEARETMGKVILAT
                     VEGDIHEIGKTLVGTMLSASGFEVTDLGVDQPADKIIGKALEIDAQIIGLSALLTTTM
                     VRQREIIEELDKENLRPRIKVMVGGAPITRDWATKIKADGTSEDAVGAVALAKQLVGK
                     A"
BASE COUNT          589 a          462 c          503 g          466 t
ORIGIN      
        1 actctggctc attttcagcg tgcccagcct ccgtttaagg aagaggcgga tcttatcctt
       61 gcgagttttt cagaggaagc ggaaccaaat ccagacgagc cggaatccgc tacgaatcgc
      121 aaaccatctt ctcctgaaat tgtttgggag gctgtacaag acgccttatt tagcgaaaaa
      181 ccaaaactgc gctatttagt gggcacacat tgggaaggaa atcgcgtgct taacgctttg
      241 atgtcaaaac tgttggatga aaatgacaac ccacagcata actattcacg ggatgaattg
      301 attgcattgt tagatcaaca tctgacagag cgaaacgctt cttagttagg cacttccgaa
      361 aaccgtctag attatatata atgtacaatt atggtaatgg cagcaaaaga gctttcacat
      421 aaacccttaa aagaggaaat ttacgatgcc ttgcaccggc agatcattgc ggggcggtac
      481 ggtcctggcg actggctgag gcaggaagat atcgccagcc agatgggagt cagcatgacc
      541 cccgtccgcg aggcgcttga ccttttggtc gcaaaaggca tcgcggagcg tgtcccctat
      601 cgcggcgtgc gtgtgcggga aatgtcgcca gaagatgtgg tggaggcgta tggcatgcgc
      661 ctctttcttg aggcgatggt ggctcgggaa accgcgctta atattacgcc tgagcaggtg
      721 caaaagcttg aaaacatcat ggcagagatg gatcgccatg tagagttgaa cgaaatgcct
      781 caagaacgcc aactgagccg tgaatttcat agcgcaattg cagaggcatc aggaaataaa
      841 ttattagtag agttgtattc catcgtctca aacgcatttc cagattggtt actgtacgaa
      901 gctctttatc gtcaacctga attagtttca agcagtgtct cacagacgca cgatgaacat
      961 gcagccattt tgaatgcatt caaaaaacat gaccctgatt tagccgtaaa agtctctctg
     1021 gagcatgtca tggaatctgg cagatggttg gaaaattatc gtaacatccc ggcaaagtta
     1081 ttaagagaaa aagaaaaaca agtttcgcac ttgatcaaaa aatccaagta aattcatcag
     1141 gaggcagtat gtcagaagaa atcgagaaat tattcaagga tatggcacaa agcattattg
     1201 acggggactc tgatctttca gtggaactcg ccgaaaaatc cattgcaatg aatatccccc
     1261 cgcttgatgc catcacaaaa ggatttgtga tcggcgtcaa ttacatcggt gaccagttcg
     1321 gcgcaggcga agcctttttg ccagaattgg ttatggcagg tgaagcgatg aaagccgctg
     1381 tagcaaccct cgagcccgag ttgctcaaac tcggtgaagc ccgcgagacc atgggcaagg
     1441 tcattctcgc caccgtagaa ggtgacattc acgaaattgg taagaccctg gttggcacca
     1501 tgctcagcgc ctctggcttc gaagtgaccg acttgggcgt agaccagccc gcagataaga
     1561 tcattggcaa ggcgttggaa atcgatgcac aaattatcgg tttgagcgca ttgctcacca
     1621 ccaccatggt gcgtcagcgc gagatcatcg aagagctcga taaggaaaat ctgcgcccgc
     1681 gtatcaaggt catggtcggc ggagcaccga ttacccgcga ctgggcaacc aaaatcaaag
     1741 cagacggaac ttcagaagac gcagtcggtg ccgtggcatt ggcgaagcag ttggttggga
     1801 aagcataagc gcaaaacaaa cgtattgaaa cctcttttct gttaatcaca ggaaacccaa
     1861 ttgctttcag caaacaagca gtacgaaatg agtaacgagg cgtaagaaaa ttgatttgct
     1921 ccctcgttac tcgtttctgt tctctccacc aaagagaact taatcccact tagaggtgtc
     1981 tcatgagacc aaagctaaca ttacttagcg acgagatcat
//
LOCUS       DAOREF010000634         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_44997_length_2019_cov_4.231670, whole genome shotgun sequence.
ACCESSION   DAOREF010000634 DAOREF010000000
VERSION     DAOREF010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44997_length_2019_cov_4.231670"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKI33_12370"
     CDS             <1..319
                     /locus_tag="PKI33_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37856.1"
                     /translation="GLILDEIVRKEQIQIDDDALNAEYMNAINSLAMQGMDFNKIRGG
                     KKGQQELSQAIAMDAASRVMTRKALDMLKSIATGSYKPFEEREAEAKKAAEAETSEEK
                     AAE"
     gene            494..1105
                     /locus_tag="PKI33_12375"
     CDS             494..1105
                     /locus_tag="PKI33_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNM37857.1"
                     /translation="MIPDFKNVVPMVVENTGRGERAYDIYSLLLRNNIIFLGTPIDDQ
                     VANVIVAQLLFLNHEDPEKEIRMYINSPGGVIYAGLAIYDTMQIISNPISTVAVGVTA
                     SFGTVLLAAGTKGRRYALPHATVHMHQPLGGAQGQATDIEIQAKQILRLKSLLNGIMA
                     KNTGQPLEVIERDLDRDYYLDAQQAVDYGLVDQIIETPEKAFK"
     gene            1239..2018
                     /locus_tag="PKI33_12380"
     CDS             1239..2018
                     /locus_tag="PKI33_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNM37858.1"
                     /translation="MIPSNIKALILDMDGVIWKGDAPIGDLPATFKRIRERGLKFVFA
                     TNNGTKTPEDYQEKLAELGVDVDPSQVVTSALGVAFMLAQRHPRGTKVFMIGENGIRV
                     ALEEKGFEVVGVEDAPQAEAVVMGIDRGISFQKVAEATLLVRAGRPFYTTNTDRTFPT
                     PRGEIPGSGSWLSVVTSATGVEPMVAGKPFPFLMELSLERLGTSKEETLVVGDRLETD
                     IAAGQAVGCPTAFVLSGVSKLEEAKEWNPSIIADSLAKLVE"
BASE COUNT          536 a          497 c          535 g          451 t
ORIGIN      
        1 tggtttgatc cttgatgaaa tcgtgcgcaa ggaacagatt cagatcgacg atgatgcctt
       61 gaatgcagaa tatatgaatg ctatcaacag cctcgctatg caaggcatgg actttaacaa
      121 gatccgtggc ggaaagaaag gtcaacaaga gttgagccaa gccattgcca tggatgcggc
      181 aagccgtgtg atgacccgca aggcattgga tatgttgaag agcatcgcga ccggtagcta
      241 caagcccttt gaagaacgcg aagccgaagc caagaaagcc gctgaagctg aaactagcga
      301 agaaaaagcc gcagagtaag aaaaggcgtt tcgaaggctg gcaggcttag gtccaagaaa
      361 tgaagcgtgc gccgcgtccc atcgggtaga atcctctcat cgaaccgggc gcggcgcatt
      421 tatatcgcct atcaaactct aatataaatc caatagtccc tcggtaacat tggtcgaata
      481 aaggagaccg aacatgatcc cagatttcaa aaatgtagtt cccatggtgg ttgaaaatac
      541 aggtcgcggc gaacgcgctt atgacattta ctcgctgctc ctgcgtaaca acatcatctt
      601 cctcggcacg cccatcgacg accaggtcgc gaatgtgatc gttgcccagt tattgttcct
      661 taatcacgaa gaccctgaaa aagaaatccg catgtatatc aactcgcctg gtggcgtgat
      721 ttatgcaggg ctcgcgatct atgacacgat gcagattatc tcgaacccga tcagcaccgt
      781 tgctgtgggt gtgaccgcct ctttcgggac cgtgttactg gctgctggaa ccaagggtcg
      841 acgctacgcc ctgccccatg ccaccgtcca catgcaccag cctctcggcg gcgcacaagg
      901 tcaggcgaca gatatcgaaa ttcaggcgaa gcagattttg cgcctcaaga gcctgctcaa
      961 tggcattatg gcgaagaaca ccggtcagcc gctcgaagtg atcgaacgcg acctcgaccg
     1021 tgattactat ctcgatgctc aacaggctgt ggattacggc ttggtggatc agatcatcga
     1081 aacccctgaa aaagcattca aatagttatt aatacggtag ctatgagacc tcgaagagaa
     1141 atcttcgggg tctttctttt ataggtataa tgcccgctgc gttgttcgtg atacgtaaac
     1201 cgtacatcga aaatctaaaa tcgcaaactg taaattgtat gatcccttca aatatcaaag
     1261 cacttattct cgacatggac ggtgtgatct ggaaaggcga cgcccccatc ggtgacctac
     1321 ccgctacatt caaacgaatc cgtgagcgcg ggttgaagtt cgtattcgct acaaataatg
     1381 ggaccaagac gcctgaagat tatcaagaaa agctagccga actcggcgtg gacgttgacc
     1441 cttcacaagt cgtcacatcg gcattaggcg tggcattcat gttggcacag cggcatccgc
     1501 gtggaacgaa ggtttttatg atcggtgaaa atggcatccg tgtggcgttg gaagaaaagg
     1561 ggtttgaagt cgttggcgtg gaggatgccc cacaagcaga agccgtagtg atgggcattg
     1621 accgtggcat cagcttccaa aaagttgccg aagccacttt gctggtgcgg gcgggtcgtc
     1681 cgttttacac gaccaataca gaccgaacct tccccactcc gcgcggagaa atccccggtt
     1741 ctgggtcttg gctctctgtg gtcacatcgg ctacgggtgt ggagccaatg gtcgctggca
     1801 aacctttccc cttcttaatg gagctttcgc tcgaacggtt gggaacatca aaagaagaga
     1861 ctctggtcgt tggcgaccgc ctcgaaacgg atattgcggc aggtcaggct gtgggctgcc
     1921 caaccgcatt cgtattgagc ggggtttcca aactcgaaga agcgaaagag tggaatccga
     1981 gcatcattgc tgatagtttg gcaaagttag tggaataaa
//
LOCUS       DAOREF010000635         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45105_length_2016_cov_4.869964, whole genome shotgun sequence.
ACCESSION   DAOREF010000635 DAOREF010000000
VERSION     DAOREF010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45105_length_2016_cov_4.869964"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PKI33_12385"
     CDS             <1..1707
                     /locus_tag="PKI33_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HNM37859.1"
                     /translation="AHWFGTDDAGQDVLTNFIYGARVSLTVGFFAAFISIVIGGLFGL
                     VAGFFGGRWENFLMRFTDIMLVIPDLPLTVVIVALTKPSLWNIIFVIGILGWTTTARV
                     VRSQTLAVKSRKFVLRARAIGASKIHILVQHILPLVLPILVVQAVLAISLAILNESTL
                     SFIGLGDPSAPSWGQMLNFAFGRGAMSVGAWWALFAPGFGIVWVVLSLTLLGQGLEQV
                     LNPRLDTHHLMPGKPVVQNEAGAKPIQQDALLEVQNLSINYVTEGKAPARAVENVSFT
                     LKAGELIGLVGESGCGKTTLMLALLKLLPSAGQIVNGKVFYSGQDLTVMNEDEINTVR
                     WSGVSIVFQGAMNALNPVRTVGDQIGEAILKHEPSFPKDKINNRVSQLLELVGIASAH
                     RDHFPHQYSGGMRQRAMIAMALACNPKVIIADEPTTALDVMIQAQILELLDSLRKKLG
                     LAVIFVTHDLGVVAELCDKVLVMYGGVTAEYADVDVIYNSPQHPYTQELLKAFPDLTK
                     PKKKLSSIPGYPPRLDNLPQGCRFAPRCPAAFDRCHTEEPLLHTIANGHVVSCHLIEK
                     SK"
     gene            1704..>2016
                     /locus_tag="PKI33_12390"
     CDS             1704..>2016
                     /locus_tag="PKI33_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM37860.1"
                     /translation="MKNTTEKLLEIRGLKKYFEAGRPSLFSRETKHVHAVDGVNLTLR
                     RSEVIALVGESGCGKSTLGLLLMGLEDPTDGSVIFEDRDITHLNDHQRKDLRRKIQMV
                     FQ"
BASE COUNT          461 a          476 c          522 g          557 t
ORIGIN      
        1 gctcactggt ttggcacaga cgatgccggg caagatgtgc tcacgaactt tatctacggc
       61 gcgcgtgtct cattgaccgt aggtttcttt gccgctttca tttccattgt cattggcggc
      121 ttatttggct tggtcgctgg cttcttcggc gggcgttggg aaaacttcct gatgcgcttt
      181 acagacatca tgctcgtcat cccagacctg ccattgacgg ttgtgatcgt agcgttgacc
      241 aagccttctc tctggaacat tatttttgtc atcggcattc ttggctggac gacgaccgca
      301 cgtgtagtcc gttcgcagac cttggcggtg aaatcgcgta aatttgtttt acgtgctcgt
      361 gccattggcg caagtaaaat ccatattctg gttcaacata ttctgccgtt ggttctgccg
      421 attttggtgg ttcaggcagt tcttgcaatt tcgcttgcca tcttgaacga aagcacgctg
      481 tccttcatcg gtttgggcga cccttctgca ccttcttggg ggcaaatgct caacttcgcc
      541 ttcgggcgcg gagcgatgtc tgtcggtgca tggtgggcgt tgtttgctcc gggctttggc
      601 attgtatggg ttgtcctctc gctcactttg cttgggcagg gcttggaaca ggtactcaac
      661 ccacggctgg atacacatca cttaatgccg ggcaaacctg tcgtccagaa tgaggcgggg
      721 gcgaagccta tccagcaaga tgctttgctg gaagtccaaa atctttctat caactatgtg
      781 accgaaggaa aagcgcctgc gcgtgcagtg gagaatgtta gtttcacgtt gaaagcgggc
      841 gagttgatcg gtttggtggg ggagagcggt tgcggcaaga caacactgat gttggcgttg
      901 ttaaaactat taccgtctgc tgggcaaatt gtgaacggca aagtttttta tagcgggcaa
      961 gatctcaccg ttatgaacga agatgaaatc aatacagttc gttggagcgg tgtttcgatt
     1021 gtgtttcaag gtgctatgaa tgcgctaaac cctgtgcgca ctgttggtga ccaaattgga
     1081 gaagccattc tcaagcacga gccatctttt ccgaaagata aaatcaataa ccgtgtttct
     1141 cagcttctgg aattggttgg gatcgcttcg gcgcatcgcg accatttccc gcatcaatat
     1201 tctggtggga tgcgtcaacg cgccatgatt gccatggcat tggcatgtaa cccgaaagta
     1261 atcattgccg acgagccgac tacagcattg gatgtaatga tccaagcaca aattttggaa
     1321 ttgctggata gcctgcgcaa gaaacttggg ttggcagtta tctttgtgac ccacgatctt
     1381 ggtgttgttg ccgagttatg tgataaagtg cttgttatgt atggcggtgt gaccgctgaa
     1441 tatgctgatg tagacgtaat ctacaattct ccgcaacacc cctataccca agaacttttg
     1501 aaagcctttc cagatttaac gaagccaaag aaaaaactct cttctattcc gggctacccg
     1561 ccgcgtttag ataacctgcc gcaaggctgt cgctttgctc cgcgctgccc agcagccttt
     1621 gatcgttgcc atacagaaga gccccttctt cataccatcg caaacgggca tgtagtgagc
     1681 tgccacctga ttgagaaatc aaaatgaaga atactaccga aaaacttttg gaaattcgtg
     1741 gtctgaaaaa atattttgaa gcggggcgcc catccttgtt ttcacgcgaa acgaaacatg
     1801 tccacgctgt ggatggtgtg aacctgacct tacgtcgcag cgaagtaatt gccctcgttg
     1861 gcgaaagcgg atgcggcaaa tcgaccttgg gcttgttgct gatgggtttg gaagacccca
     1921 ccgatggctc ggttattttt gaagatcgcg atattacaca tctcaacgac catcaacgaa
     1981 aagacctgcg ccgaaaaatt caaatggttt ttcaag
//
LOCUS       DAOREF010000636         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45143_length_2015_cov_4.699490, whole genome shotgun sequence.
ACCESSION   DAOREF010000636 DAOREF010000000
VERSION     DAOREF010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45143_length_2015_cov_4.699490"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..772)
                     /locus_tag="PKI33_12395"
     CDS             complement(35..772)
                     /locus_tag="PKI33_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37861.1"
                     /translation="MSKYKYDYYDDAPEESKAPMRLDIWDMLSILGILATLCIGAYFA
                     AVFVSPNASYNLLNPVRNQPPTPTITQIQPPATWTPTPLGPTETATLTLVPTFTLEPS
                     PTVVSLITPSVTPTATRTPKAPFFATVTYIDSTIIHPEAACNWQGVAGTIIDANNADM
                     LGIAIRLTGFYNGKSKNELTVSGIAPAYGKSGFEFFLSAVPVASDNLLTIQILDQAGL
                     PLSDNIPINTYADCTKNMVLVKFKKNP"
     gene            complement(795..1652)
                     /locus_tag="PKI33_12400"
     CDS             complement(795..1652)
                     /locus_tag="PKI33_12400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNM37862.1"
                     /translation="MTLFLAVGISACGSLGTSSTPTPSEPTPTLAPPTPTPPPSAAAV
                     NGEYITITEFQAELERYKAAQTALGLSFTDEDANKVVLEDMIAQVLLSQAARAENFEV
                     TDADLQSRIDALVSQLGSADALIQWQSAHGYDDASFRLALKRSIEAAWMRDKIVAAVP
                     TSAEQIHLRQILTYNEADAQSALAELNAGTDFDELAAQYDPVTAGELGWVPQGYLLDP
                     KADEAVFALQTGQVSGIISTDAGFHIFKAIERGDHALSPDALLAMQEHALQNWVADQR
                     AKSEIVIAP"
     gene            complement(1749..>2015)
                     /locus_tag="PKI33_12405"
     CDS             complement(1749..>2015)
                     /locus_tag="PKI33_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNM37863.1"
                     /translation="DGSVIIDTVRRTAAEKKNPNIGYNVLTSEYTDMIKAGVIDPVKV
                     VRGALENAASIAAMILTTDVLITDMPAKENAPAMPPGGMGGMDY"
BASE COUNT          539 a          472 c          516 g          488 t
ORIGIN      
        1 aaaaaatccg ccgaatactc ggcggatttt ttatctacgg gttcttctta aatttcacca
       61 ataccatatt cttcgtacaa tctgcatagg tattgatcgg aatattatca gaaagcggca
      121 accctgcctg gtcaagaatt tgaatcgtga gtaagttatc agacgcaact ggaaccgcgc
      181 tcaagaaaaa ctcaaaaccc gattttccat aggctggagc aataccgctc acagtaagtt
      241 cattcttgct tttaccgtta tagaagcctg tcaaacgaat agcaatgcca agcatatctg
      301 cattattcgc atcaataata gtaccagcca caccttgcca gttacaagca gcttccgggt
      361 ggataatggt gctatcaatg tacgtcactg tagcaaagaa aggagccttg ggtgtacgtg
      421 ttgcggttgg agtaacagac ggggtaatca gggaaaccac tgtgggagaa ggttcaaggg
      481 taaatgtagg caccaatgtt aatgtggcag tttccgtcgg tccaagcgga gtgggcgtcc
      541 atgtggcagg cggttgaatt tgtgtaatcg taggagtagg aggttgatta cgaactgggt
      601 tcaacaaatt gtatgaggca tttggtgaga caaacacagc cgcaaaataa gcaccaatac
      661 aaagagtagc caaaataccc agaatgctca acatatccca aatatcgagc cgcatgggcg
      721 cttttgactc ctcaggggca tcatcataat agtcgtattt gtatttgctc ataaatcact
      781 ccaaaaaatc acacttaggg tgcgatgaca atttcacttt tcgctcgttg gtcggcgacc
      841 caattttgta aagcatgttc ttgcatggca agcaaagcat caggcgaaag ggcatggtcg
      901 ccgcgctcaa tcgctttgaa gatatggaaa cccgcatccg tagaaataat gccacttact
      961 tgacccgttt gtaaagcaaa aacggcttca tccgctttgg ggtctagcaa atatccttgt
     1021 ggcacccaac ccaattcgcc agcagtcact gggtcatatt gagccgccaa ctcatcaaaa
     1081 tctgtgccag cattaagttc agccaatgct gattgcgcgt ccgcctcatt ataggtcaaa
     1141 atctggcgca aatgaatttg ctcggcagat gtaggcacag ccgcaacgat tttgtcacgc
     1201 atccatgccg cttcaataga acgctttagt gccaagcgga aggatgcatc atcataccca
     1261 tgcgcagatt gccattgaat aagcgcatcg gcactgccaa gttgacttac caacgcatca
     1321 atacgtgact ggaggtctgc atcggtcact tcgaagtttt ctgcccgcgc agcctgactc
     1381 aacaatactt gcgcgatcat gtcctcaagg acaaccttgt tcgcatcctc atcagtgaaa
     1441 gaaagtccca gcgcagtttg ggcggcttta tagcgttcca gttccgcttg gaattcggtg
     1501 atggtgatgt attctccatt gacagccgct gcagaaggcg gaggagtagg agtaggcggt
     1561 gcgagagtgg gagtcggctc agaaggggtg ggagtgctcg aggtcccgag agaaccgcag
     1621 gcagatatcc ccacagcgag gaacaaagtc aaaaatatcc gtttgaattg cattcgtaaa
     1681 attataaatt aaaaacgtag gggcgacccg ttgggtcgcc cctacaaatt tgtggggaaa
     1741 ggattggttt agtaatccat gccgcccatg ccgccaggag gcatggcagg agcgttttcc
     1801 ttcgcaggca tgtcggtgat gagcacgtct gtggtgagga tcatcgcggc gatggaagca
     1861 gcgttttcga gcgcaccgcg cacgaccttg accgggtcaa tgacgccagc cttgatcatg
     1921 tcggtgtatt cgctggtgag gacgttgtaa ccaatgttcg ggttcttctt ctcagcagcg
     1981 gtgcggcgga cggtgtcgat gatgacggag ccatc
//
LOCUS       DAOREF010000637         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45200_length_2014_cov_4.115365, whole genome shotgun sequence.
ACCESSION   DAOREF010000637 DAOREF010000000
VERSION     DAOREF010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45200_length_2014_cov_4.115365"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="PKI33_12410"
     CDS             complement(<1..118)
                     /locus_tag="PKI33_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM37864.1"
                     /translation="MLIERVVGAVRAVDNISFTIARGETLGMVGESGCGKTTA"
     gene            complement(175..1170)
                     /locus_tag="PKI33_12415"
     CDS             complement(175..1170)
                     /locus_tag="PKI33_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM37865.1"
                     /translation="MKTDHKFLEVKDLKTQFNTPEGTVYAVNGVSFSLNEGETLAVVG
                     ESGCGKSVTMMSIMKLIQMPPGKIVGGEVIFRDSDLLKLSEEAMEQIRGREIAMIFQD
                     PMTSLNPVLTVGRQITETLRTHTPMTPEQCRDRAAQLLELVGIPDPVKRLDDYPHQFS
                     GGMRQRVMIAMALACTPSLLIADEPTTALDVTIQAQIVELVKDLKKSVGMAMIWITHD
                     LGVVAGMADRVLVMYAGYVVEEAPVDDLYENTLHPYTLALLQALPRADRRRDQSLKSI
                     KGSPPNLLMEPKGCPFAPRCEFAQEKCIHENPTLAEFEPNHKVACWVDVKTGALR"
     gene            complement(1177..>2014)
                     /locus_tag="PKI33_12420"
     CDS             complement(1177..>2014)
                     /locus_tag="PKI33_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37866.1"
                     /translation="IVGLGVLILLIIYSFGGAFFVTEKYSNFTNTKERLQPPSAEHPF
                     GTDTVGRDLMARTIYGGQISIIIGITAAFVEVMMGILIGAIAGYFGGKIDAFLMRFTE
                     AMLNLPQLLLLIVMAKFFSSDIKNINLFGREFSGSVVVIIFIIGLTSWPYLARIVRAE
                     FLSIKEREFILAARATGTPTWEIIFKHILPNSIAPIVVSATLGVAGAISLEAYVSFLG
                     LGVRAPTATWGNMLENAYRYIESAYWMWLFPGLFIVLIVLSINFLGDGLRDALDPRSR
                     TV"
BASE COUNT          564 a          527 c          423 g          500 t
ORIGIN      
        1 cggcggttgt cttaccgcag ccactttcgc cgaccatgcc caaggtttca ccgcgcgcaa
       61 tggtgaatga aatattgtcc acagctcgta cagcgccaac cacacgttcg atcaacattc
      121 cttttcgaat tgggaaatgt ttggtcaggt tttcaactcg taccaatggt tcgctcatcg
      181 caatgctcca gtctttacat ctacccagca ggcgacttta tggtttggtt caaattcagc
      241 taatgtaggg ttttcatgga tgcatttttc ttgagcgaat tcacagcgtg gcgcgaaggg
      301 acatccttta ggttccatca acagatttgg cggagagccc ttaatagatt tcaagctttg
      361 gtcgcgtcgg cgatctgctc gtggtaaagc ttgcagtaac gccaaggtat agggatgcaa
      421 tgtattttca tacaggtcat ccacaggagc ttcttccact acatacccgg catacatcac
      481 aagcacacga tctgccatac ctgccacaac acctaaatca tgcgtgatcc aaatcatggc
      541 catgcccacg cttttcttta ggtctttcac caattcaacg atctgcgcct gaattgtcac
      601 atccaaagct gtggtcggtt catctgcgat caacaaactg ggcgtgcaag ccaacgccat
      661 agcaatcatc acacgctggc gcatgccacc agaaaactga tgtggataat catccaaccg
      721 tttgactggg tctggaatcc caacaagttc taataactga gcagctcgat ctcgacactg
      781 ttcaggcgtc ataggagtat gagtgcgcag cgtttctgtg atttgacgac ccacggtcaa
      841 cacaggattt agactggtca ttgggtcttg gaagatcatc gcaatctcac gcccgcggat
      901 ttgttccatc gcctcttcac ttaatttgag caggtcgctg tctcgaaaga tcacttcccc
      961 accgacaatt ttcccgggtg gcatctgaat taatttcatg atggacatca tcgtcaccga
     1021 tttgccacaa ccactttcgc ctacaacagc tagtgtctca ccttcgttca agctgaacga
     1081 aacgccattc acagcataga cagtcccttc cggtgtattg aactgcgtct tgaggtcttt
     1141 tacttcaagg aatttatggt ctgttttcat aagtcattac accgttcggc tacgaggatc
     1201 aagagcatca cgcaagccat cacccaaaaa gttgatgcta agcacgatca aaacaataaa
     1261 taaaccgggg aacagccaca tccaatacgc agactcaatg tagcgatagg cgttttcgag
     1321 catattgccc caagtcgctg tcggcgcacg aacgcctaag cctaaaaagc tgacatacgc
     1381 ctcaagggag attgcgcccg caacgcccaa ggtagcggat acgacgattg gagcaatgct
     1441 attcggcaga atatgcttga aaataatctc ccaagtaggg gttccagttg cgcgtgccgc
     1501 aagaataaac tcgcgttctt tgatagacag aaattcagca cggacaatac gcgccaaata
     1561 tggccaactc gtaagtccaa tgatgaagat aattacgacc acactgccgc taaactccct
     1621 tccaaagaga ttaatatttt taatatcact ggaaaagaac tttgccatta cgatcaacaa
     1681 aagcaattgc ggcaaattca gcatggcttc ggtaaaacgc atcagaaagg catcgatctt
     1741 cccaccaaaa tagccagcaa tcgccccaat gagaattccc atcatcactt ctacaaaagc
     1801 ggctgtaatc ccaataatta tagagatttg cccgccataa attgtacgag ccataaggtc
     1861 tcgtccaacg gtgtctgttc cgaagggatg ttccgcgctc ggcggttgta gtcgttcttt
     1921 ggtattggta aagtttgaat acttctccgt aacgaaaaac gctccgccaa aagaataaat
     1981 aatcaaaaga ataagaaccc ccagcccaac aatc
//
LOCUS       DAOREF010000638         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45319_length_2011_cov_3.349693, whole genome shotgun sequence.
ACCESSION   DAOREF010000638 DAOREF010000000
VERSION     DAOREF010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45319_length_2011_cov_3.349693"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PKI33_12425"
     CDS             complement(<1..507)
                     /locus_tag="PKI33_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNM37867.1"
                     /translation="MTTSAISETKPNFIRSILAWGVHLFTATGAIWGLLGIFAIFENN
                     YKMMIVWMIVAMLVDGFDGMLARWADVKKYANGIDGALLDNILDYLNYVLVPAIFMIH
                     TDLLPEPVKWLTACLILLTSAYQFTQTDAKTDDHHFKGFPSYWNVAALYMLLMNLPPW
                     VNFGFLMLF"
     gene            648..1148
                     /locus_tag="PKI33_12430"
     CDS             648..1148
                     /locus_tag="PKI33_12430"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNM37868.1"
                     /translation="MKLFISILFFLILAVVFFWQSYRLLSRSLPGLRDAQASRSWHSV
                     QGTITIVSSRMEWVNIKRKNLSYFRPWVQSTYTVNGQALVCERFAFNDHLISASTKKA
                     AEDHVKSYHIGQSVQVYYDPNDPQKSVLEPGNLSPSMPSLLGGVVFFILALLQFVMAF
                     LAIKQA"
     gene            complement(1234..1671)
                     /locus_tag="PKI33_12435"
     CDS             complement(1234..1671)
                     /locus_tag="PKI33_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37869.1"
                     /translation="MPIIHKIDPEHGLLYILLHGRVAMADVVTYEKLLLDDPLRQPNL
                     KILADLLLADMEVDLDSLKKISARHRSLHESGWKMEQTAVLTNNRMLELLGDAYELMS
                     FGVPVHFKIFSVLRDAILWLELRQSQEQIKSIRQQLVDRVSSS"
     gene            complement(1751..>2011)
                     /locus_tag="PKI33_12440"
     CDS             complement(1751..>2011)
                     /locus_tag="PKI33_12440"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNM37870.1"
                     /translation="VAEGLAREFVRRVQDLRKTANLDVADRIELFVEASAGLKTAVEA
                     HADYIKAETLTVKLSFAAAPANASVVEDEFEGEKVKVGLLKN"
BASE COUNT          549 a          486 c          441 g          535 t
ORIGIN      
        1 gaagagcatc agaaaaccga agttgaccca gggcggcaaa ttcatgagca acatatacag
       61 tgcagcaaca ttccaatagg acgggaagcc tttgaagtga tggtcgtcgg tcttggcgtc
      121 tgtctgggtg aattgatacg cagacgtgag caggatgaga caagccgtaa gccatttgac
      181 tggctcaggc agcagatctg tgtggatcat gaaaatggca gggacgagga catagttgag
      241 atagtcgaga atgttatcga gcagagcacc atcgatacca ttggcatatt ttttcacgtc
      301 tgcccagcgc gcgagcatgc catcgaagcc gtccacgagc atggcaacga tcatccagac
      361 gatcatcatc ttgtagttat tctcaaagat ggcaaagatg ccaagcaagc cccagatagc
      421 acccgtagca gtaaagaggt gcactcccca ggcaaggatg gagcgtatga agttaggttt
      481 ggtttcagaa atcgcagatg tggtcatatc cagtccttta gatagtttca gtatttaaca
      541 ctagagatta taaaaggaaa ctgttttttt tcagaataaa gattagaagg tgtaaggtaa
      601 aatattttca ctctgtagaa ttgctaaatg gacgttattt attccacatg aaactattta
      661 tctccatcct ctttttccta atcctcgccg tggttttctt ttggcaaagt taccgtctgc
      721 ttagtcgcag cctaccgggg ctgcgtgatg cgcaagccag ccggtcgtgg cattcagtac
      781 aaggaacgat cactatcgtc tcctcccgca tggagtgggt caatatcaaa cgcaagaatc
      841 tatcgtactt tcgtccgtgg gtacaaagta catatacagt caatgggcaa gccctagtct
      901 gtgagcggtt tgccttcaac gaccatctga tctctgccag cacaaagaaa gctgcggaag
      961 accatgtcaa atcctatcat atcgggcaaa gtgttcaggt ctattacgac cctaacgacc
     1021 cgcaaaagtc tgttctggag ccggggaatt tatctccttc tatgccatca ctgttaggtg
     1081 gggtcgtttt tttcatcctt gccctgctcc agtttgtgat ggctttttta gcgataaaac
     1141 aagcttgatt tggaattttt aaacaaagac ctccgagatt ttataaaatc tcggaggtct
     1201 ttttacttac agccactagg tctaaaacct tttttacgaa gaagaaacac gatctaccaa
     1261 ttgctgccga atggatttga tctgttcctg tgattgcctt aactccagcc ataaaatagc
     1321 atcacgtagt acggaaaaaa tcttgaaatg cacaggcacc ccaaaagaca tcaactcata
     1381 ggcatcgccg agcagttcta gcatgcggtt gtttgtgagt acagccgtct gctccatttt
     1441 ccagccagat tcgtgcaagc tgcgatgccg tgcacttatc tttttcaaac tatccaggtc
     1501 tacttccata tccgctaaca aaaggtctgc cagaatcttc aaattcggct gccgtaatgg
     1561 gtcatctagc aataactttt cgtaggtcac cacgtctgcc atggcaactc taccgtgcaa
     1621 caagatataa agaagcccat gttcagggtc aatcttatga ataatcggca tcttagatca
     1681 tcctattttc aaaacaagta atcccctcgg taaaaaatca ctcacccgag gggattaact
     1741 ttttcaagca ctaatttttc aacaacccca ctttcacttt ctcgccttca aactcatctt
     1801 ccaccactga agcattcgca ggcgcggcag cgaaagataa cttaaccgtc agcgtttcgg
     1861 ctttgatgta atccgcatgt gcttccacag cagttttcaa gccagcgctg gcttccacga
     1921 acagttcgat gcggtcggca acgtcgagat ttgcggtctt gcgcaagtct tgcacgcggc
     1981 gcacaaattc acgtgccagc ccttcggcaa c
//
LOCUS       DAOREF010000639         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45352_length_2010_cov_5.033760, whole genome shotgun sequence.
ACCESSION   DAOREF010000639 DAOREF010000000
VERSION     DAOREF010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45352_length_2010_cov_5.033760"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKI33_12445"
     CDS             complement(<1..314)
                     /locus_tag="PKI33_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37871.1"
                     /translation="MRRLFLPLLLTVLACGGNGIPGPTPTPLSPTATVSAPQTESQPQ
                     ASPATLPPTEAVIESTQQFPDPTAYEWKTLVEGLDRPVDVQSAQDGSGRLFVIEKYGA
                     IR"
     gene            complement(315..686)
                     /locus_tag="PKI33_12450"
     CDS             complement(315..686)
                     /locus_tag="PKI33_12450"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNM37872.1"
                     /translation="MTEKKSSDIIVNQQGGVVRNLVNQLKLIFRLMGDKRVSVLAKLV
                     PLGAFAYLIMPADLAPNVVLPVIGMVDDAAILWLGTYIFTELCPPEVVEEHMKALAGN
                     MNPKDVNNEDVVDGETTEVKE"
     gene            906..1298
                     /locus_tag="PKI33_12455"
     CDS             906..1298
                     /locus_tag="PKI33_12455"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNM37873.1"
                     /translation="MDMIIDFPGGQKVDAHFRGHTIKTDQPNDNTAPMPFELFLASIG
                     TCAGIYVLGFCQQRNLPTAGIRIVQRNYSNPANGLVDKIDLEIQVPPNFPEQYHAALV
                     RTAELCKVKKTLEHPPVFDVMTKVVEAA"
     gene            1383..1850
                     /locus_tag="PKI33_12460"
     CDS             1383..1850
                     /locus_tag="PKI33_12460"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNM37874.1"
                     /translation="MPYIIDGHNLIPKLGLRLDEPDDEMELVRLLQDFARIRRQQIDV
                     YFDGAPAGQAGMRKFGSIKAHFVRQGQTADSAIRRRLEEMGKAAKNWIIVSSDHEVQS
                     AARVHMAQSIRSEEFVKQIRIAFSTSTQTGASDSKLSAQEVEEWINLFSKKKE"
BASE COUNT          562 a          476 c          473 g          499 t
ORIGIN      
        1 atacggatcg cgccgtattt ttcgattacg aataaacgcc ccgagccatc ctgcgcagac
       61 tgcacatcta cggggcgatc caaaccttcc acgagagtct tccactcgta agcagttggg
      121 tcggggaatt gctgggtgct ttcaatcact gcctcggttg gaggaagtgt ggcgggggac
      181 gcttgaggtt gagattcggt ttgtggtgct gaaacagttg ccgtcggaga aagaggcgtg
      241 ggagtgggtc cgggaatgcc attccctccg caggcaagga cggttaacaa taaagggagg
      301 aaaagacgtc gcatttattc ttttacttca gtggtttcac catccacaac atcttcattg
      361 tttacgtctt tggggttcat attcccggca agcgccttca tgtgctcttc cacaacttct
      421 ggcgggcaca attcggtgaa gatataggtg ccaagccaga gaatggcggc atcatcgacc
      481 atgccaatta caggcaagac gacgttcggc gcaagatcag caggcataat caaataagca
      541 aaagcgccca agggcactaa ctttgccaag acactgacgc gcttatcgcc catgagacga
      601 aagataagct tcagttggtt cacaaggttg cgcaccaccc cgccttgttg gttgacgatg
      661 atatcagagg attttttctc agtcattttg ttctcctttg agttgtatat tcattatatt
      721 cggaattgca gtacatccac tttcgattat taccgtttgc atgaacatag attgatatac
      781 gtgacaaaag aagatggggt gcaggtaaaa atggcagaag caggaaaaaa actcaaaaat
      841 tgggtgtaac ttattcttag caaatacatc caatatgtac aacaataaat ttggaggatt
      901 tccccatgga tatgattata gatttccccg gcgggcaaaa agtagatgcc cacttccgcg
      961 gtcacaccat taagacagac caacccaacg acaatactgc tccgatgccg tttgaattgt
     1021 tccttgcttc tattggtacc tgtgcaggca tttatgtgct aggcttttgc caacagcgca
     1081 acctgcccac tgcaggcatt cgcatcgttc agcgcaatta ttccaacccg gcaaacgggt
     1141 tggtcgataa gatcgatctt gaaattcagg taccacccaa cttccccgaa caataccatg
     1201 ccgcactcgt gcgcactgca gagttatgca aagtgaagaa gactcttgaa catccacctg
     1261 tgtttgatgt aatgaccaaa gtggtcgaag ctgcctaaga aaccgaagat aaaaagaccg
     1321 cagacattca cagtctgcgg tctttttatt ccaaaacaac tcaagcacag taaaatgaac
     1381 ccatgcccta cattattgat ggacataacc tgattcccaa acttggattg cgtttggatg
     1441 aacccgatga cgaaatggaa ctcgtgcgcc tcttacaaga ctttgcacgg attcggcgac
     1501 agcagattga tgtctacttt gacggcgctc ccgccgggca agctggcatg cgaaaatttg
     1561 gcagcatcaa agcgcacttc gtaaggcaag gtcaaaccgc agactcagcc atccgcaggc
     1621 gattagagga gatggggaaa gcagcaaaaa attggatcat tgtttcttca gaccatgagg
     1681 tgcaaagtgc agcacgagtg catatggcac aatcgattcg ctcggaagaa tttgtcaaac
     1741 aaattcgaat tgccttctct acaagtaccc aaacgggtgc atctgactca aagctttcgg
     1801 cgcaagaagt ggaagagtgg ataaatctat ttagtaaaaa gaaagaatag tacctttgtc
     1861 ttatacgttt ttaatgaaat gtggacaaaa aaggtgtata ttgtctgtac gacacctgcc
     1921 ctccctttta ctggtattct ggtgtcaacc gccgctctgg tcaacatgcc ccccccatgc
     1981 tgacaggcgg cggtaccttt ttaacaatgc
//
LOCUS       DAOREF010000640         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45660_length_2003_cov_2.553901, whole genome shotgun sequence.
ACCESSION   DAOREF010000640 DAOREF010000000
VERSION     DAOREF010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45660_length_2003_cov_2.553901"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKI33_12465"
     CDS             complement(<1..910)
                     /locus_tag="PKI33_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM37875.1"
                     /translation="MSVSNTTPVLAVDEKHLVPVSDHLKQMMDIPPSRMFLINKSLKV
                     YQEKNAGGKIFDASQGDGGASLPGTPKPILERALQLQLEHGTAYDMPFGTEAYRKAVI
                     EKYWKLDASSGWGPANVLGTAGGRDALNKAYQAMLALGYGRQGDLIMVSRVPWISYNW
                     GPYGVGANVLWTPGDPAQGWAYSEEAIRESVKFAESKGRKIAGIVITNPDNPTGMTIA
                     VEKQVSLAKAALEAGVAFVLFDWMYHYVTDESPMDLNSFLKAFTPEERKRIMFLDGIT
                     KSLGGSNIRNCHLIADEAVIKFITARA"
     gene            complement(1134..1418)
                     /locus_tag="PKI33_12470"
     CDS             complement(1134..1418)
                     /locus_tag="PKI33_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNM37876.1"
                     /translation="MLPYHLQNLARTGREVQIAVDGAPTIGSVLEALEKQYPMLRGTI
                     RDQATGQRRPFIRFFVCGADWSLEAWDVELPLAIQNGSEVFRVVGAMSGG"
     gene            complement(1427..>2003)
                     /locus_tag="PKI33_12475"
     CDS             complement(1427..>2003)
                     /locus_tag="PKI33_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37877.1"
                     /translation="AGAFRTENGGETWLPINQGLSSGELPDADAEVGHCVHNISMHPT
                     RPNVLFMQKHWDVMRSNDAGASWHEVSGNLPSDFGFPIAVHAHEPETIYVVPIKSDSE
                     HFPPDGKLRVYRSRTGGNDWEALTKGLPQENCYVNILRSAMTVDTLDSCGIYFGTTGG
                     QVYASSDSGDSWTPIVSNLPSVLSVEVQTLK"
BASE COUNT          507 a          523 c          508 g          465 t
ORIGIN      
        1 aagcacgagc ggtgatgaat ttgataacgg cttcatctgc aatgaggtga cagttgcgga
       61 tgtttgaacc gcccaaggat ttggtaattc cgtccaggaa catgatgcgt ttgcgctctt
      121 cgggggtgaa tgccttaagg aaagaattca aatccattgg cgattcatcg gtgacgtagt
      181 gatacatcca atcgaagagt acgaaggcaa cgcctgcttc aagggcagcc ttggcaagcg
      241 agacctgctt ttcaacagca atggtcatac cggtagggtt atcagggttg gtaatgacaa
      301 tgcccgcgat cttgcgtcct tttgattcgg caaatttgac actttcacga atcgcttctt
      361 cagagtatgc ccaaccttgc gccgggtcac cgggagtcca caagacattg gcgcccacac
      421 cgtatggacc ccagttatat gaaatccacg gcacacgtga gaccataatg aggtcaccct
      481 ggcgaccata gccgagggca agcatggctt gataagcttt attgagggca tcacgtccgc
      541 cggcggtgcc gagcacattg gcaggtcccc atccagaaga ggcatctagc ttccaatatt
      601 tttcaatgac cgccttacga taggcttcag tgccgaacgg catatcgtat gccgtaccat
      661 gctcgagctg gagttgaagc gcgcgttcca gaatcggctt tggggttccg ggcaacgaag
      721 cgccgccgtc gccttgagaa gcatcaaaga tcttccctcc ggcattcttt tcctggtaga
      781 cctttaacga tttattgatg agaaacatac gggaaggcgg aatatccatc atctgcttga
      841 gatgatcgct gacaggcacg agatgttttt cgtcaacagc aagaacaggg gtggtattgg
      901 aaacggacat ggtgaattct ccttaagaaa aatgccccga ggctcggggc atttggtgta
      961 cttagtaaga ttgaacttgt tgctgttcac ctgacaacca ccaatgcccc gggcaccaac
     1021 gattggtgcg ggtagtattg gtattggtgt caacaacgaa ttgcttgaac attgcgcgca
     1081 agcataccac ttgcatatac atgcgtcaag caggaattta ggtttgcagt aacttagccg
     1141 ccagacatgg cgcccaccac acggaacact tcgctcccat tttgaatcgc aagcgggagc
     1201 tcaacatccc aagcctccaa ggaccagtcg gcaccacaaa caaaaaagcg gatgaaaggt
     1261 ctacgctgac ccgtggcttg atcgcgaatg gtgccgcgca gcatgggata ttgtttttcc
     1321 aacgcctcca acacggaccc gatcgtaggc gccccatcga cagctatctg cacctctctg
     1381 cctgtgcgcg ccagattttg aagatgataa ggaagcacaa cccgtatcat ttcaaggttt
     1441 gcacttccac agacaacacg gatgggaggt tactgacaat gggcgtccaa ctatcgccag
     1501 agtctgaaga ggcgtagacc tgcccaccag ttgtgccaaa ataaattcca caggagtcga
     1561 gggtatccac tgtcatggca gagcgcaaaa tgttgacgta acagttttcc tgcggcaaac
     1621 ctttcgtcaa cgcttcccaa tcattaccgc cggtgcggct acgatataca cgcagtttcc
     1681 catccggtgg gaagtgttct gaatcacttt taattggtac tacataaatg gtctctggct
     1741 catgggcatg gactgcgatc gggaagccaa aatcgctggg caaatttccg ctcacttcat
     1801 gccatgaagc gccagcatca ttgctgcgca tcacatccca atgtttctgc atgaacaaga
     1861 cattcgggcg tgttgggtgc atggaaatat tatgtacaca gtgacccacc tccgcatcag
     1921 catcaggcaa ttcgccggat gacaatcctt gattgatcgg caaccacgtc tcgccgccat
     1981 tttcagtgcg gaaagcccct gcg
//
LOCUS       DAOREF010000641         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45760_length_2000_cov_3.576350, whole genome shotgun sequence.
ACCESSION   DAOREF010000641 DAOREF010000000
VERSION     DAOREF010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45760_length_2000_cov_3.576350"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="plsX"
                     /locus_tag="PKI33_12480"
     CDS             complement(<1..880)
                     /gene="plsX"
                     /locus_tag="PKI33_12480"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNM37878.1"
                     /translation="MARIVVDAMGSDEHPGPDVEGAIQAAREYGVEIIFVGDEAKIKP
                     VLEKQNTQGLSVRVVHAPEMLTMEDKGEALVLKARSKEPKNSMAIGMDLVKNGEADAF
                     VTAGNTGAGMVTALFRLGRIRGVDRPALAPIFPTATGTCVVLDIGANPDCKPENLIQF
                     GILGSIYAEKVRGVKNPKVGLVSNGEEEGKGNELVKGATPLFKASNLNYFGNVEGKEV
                     IGGAVDVAVTDGFTGNVMLKTSEAVAKLITDTIRSLIKNGNLQTKIGGLLVKPALGAI
                     KKLLDPGEQGAAPMLGV"
     gene            946..1027
                     /locus_tag="PKI33_12485"
     tRNA            946..1027
                     /locus_tag="PKI33_12485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Leu,seq:gag)
     gene            complement(1062..>2000)
                     /locus_tag="PKI33_12490"
     CDS             complement(1062..>2000)
                     /locus_tag="PKI33_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM37879.1"
                     /translation="YSRAEVVNRWASELNLWQNNDEHALHHKLIRENGFVHEFKTTLR
                     KRSGDLGTVLLSVTMVRINNEDCLLYTISDISGLEKALNDLQNKNSELERFTYTVSHD
                     LKAPLITIGGFMGLLEKDLASGNIDKINNSARRIKDAVTKMERLLNELLELSRIGRMI
                     NEPIKVSFEEILREAISLVQGRLSANKIEVQIEPELPFVKVDRARVVEVIQNLLDNSA
                     KFMGQQPNPKIAIGMRRTMDGERVFVVSDNGIGIEPVYHDRVFGLFNKLDANTEGTGI
                     GLALARRIIEYHGGRIWVESEGKGKGTSFCFTLPRP"
BASE COUNT          511 a          499 c          403 g          587 t
ORIGIN      
        1 ttacgcccaa catgggggca gcaccttgtt ccccggggtc aagtagtttt ttaatcgcac
       61 ctaatgctgg tttgacgagt aatccgccga tttttgtttg cagatttcca tttttgatga
      121 ggctgcgaat cgtatctgtg atgagttttg caacagcttc agaagtcttt agcatcacat
      181 ttccggtgaa gccgtcagta accgctacat ccactgcgcc gccaattact tctttaccct
      241 ctacattacc aaaataattc aagttcgatg ctttgaataa aggagttgct cccttaacca
      301 attcattgcc cttgccttct tcttcaccat tcgagaccaa accaacttta ggattcttca
      361 cgccgcgaac tttttctgca taaatgcttc ctaaaatccc gaactgaata agattttcag
      421 gtttgcaatc agggttggcg ccaatatcca aaaccacgca agtgccagtc gctgtcggga
      481 agatgggggc tagggctgga cgatccaccc cacggatacg tccgaggcgg aagagagcag
      541 tgaccatgcc tgcgccggta ttaccagccg tcacaaaagc atctgcttct ccgtttttga
      601 cgaggtccat gccgattgcc atggagttct ttggctcttt ggagcgcgcc ttcaaaacca
      661 atgcttcgcc tttatcttcc atcgtcaaca tttcaggcgc gtgtactact cgcacactta
      721 gcccttgtgt attttgtttt tcaagaacag gcttgatctt ggcttcatcg cctacaaaaa
      781 taatttctac cccatattcc cgagctgcct gaatggcacc ttctacgtca ggtccggggt
      841 gttcgtcgct tcccattgca tcaactacga tacgagccat gaaaaatctc ctatgatgat
      901 ttgcttgaca agaaaagtaa cacgattata atactgcctg cttacgcgct ggtgctggaa
      961 ctggcagaca ggcacgtttg aggggcgtgt ggcgcaagcc gtggaggttc aagtcctctc
     1021 cagcgcacaa gcaggaactc ccaaaatggg agttctttgt tttaagggcg tggcagtgta
     1081 aaacaaaatg aggttccttt accttttcct tcagactcaa cccaaatgcg cccaccatga
     1141 tactcgataa ttcggcgggc aagagctaac ccaattccag ttccttctgt attcgcatcc
     1201 agtttgttga ataacccaaa caccctatca tgataaacag gctcaatccc gattccatta
     1261 tcggatacaa caaaaactcg ctcaccgtcc attgtcctac gcatgccaat cgctattttg
     1321 gggtttggct gttgccccat gaattttgca gaattatcta gtagattttg gatgacttca
     1381 acaactcttg cgcgatctac tttgacaaaa ggaagttccg gctctatctg gacttcgatc
     1441 ttgtttgcac ttaatcgacc ttgcactaaa ctgatagctt ctcgcaaaat ctcttcgaaa
     1501 gatactttga tcggttcgtt gatcattctg ccaatgcgcg aaagctctag aagctcattt
     1561 aataagcgct ccatttttgt gacagcatct ttgatacgcc gagcgctatt gttgatctta
     1621 tctatatttc cagaagcaag gtccttttca agcaaaccca taaatccgcc aattgtaatt
     1681 agtggcgctt ttaagtcatg agaaactgta tatgtgaatc tctctaattc cgagttttta
     1741 ttttgtaaat cattaagcgc cttctctaga ccactaatat cagaaattgt atataacaga
     1801 caatcttcat tattgatgcg aaccatcgtt acagaaagga gtacagtgcc caagtctcca
     1861 cttctcttac gcaaagtagt cttgaattcg tggacaaaac cattttctcg aatgagtttg
     1921 tggtgcaatg catgttcgtc attgttttgc caaagattaa gctcagacgc ccagcgatta
     1981 acaacttctg cgcgtgagta
//
LOCUS       DAOREF010000642         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45848_length_1998_cov_2.538343, whole genome shotgun sequence.
ACCESSION   DAOREF010000642 DAOREF010000000
VERSION     DAOREF010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45848_length_1998_cov_2.538343"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="PKI33_12495"
     CDS             complement(<1..1998)
                     /locus_tag="PKI33_12495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNM37880.1"
                     /translation="MKDSVIVLNSEKRVLYVNHVTEKLFNLTEDRVIGQPFNHVAGHL
                     ANLLIPYLKQSGPHNEIQIQEGKEEKTYEVSISPITGTNQNTSDIVITLHDITLRKEK
                     EQELSRHGLIMSAISKAAEQFLKTDNWEQHIVSVLENLGQAADVSKVVVTIEHTHNTK
                     YEWKAQDWGNVSNPISQLTIPVIVNGEPWASISFDENRRERQWTNMELEAFQTAANIL
                     AAAETRTQTVHQLNRRQLALGLLQDIATVSLQAANLNEMAEVSAERLAKLIKADGCYL
                     TLWNDVTKHVVPVAAYGTGKETYARTSAKEDEKTFTQSSLELEKTLIVEDVYNTPYAS
                     PSVVSRFPAKSLLILPLIALDQKLGAVLVSFETPHQFTDEEVQICEQAASLIALALEK
                     FQAMDEARKRADTAENLRKVGLAVAEQTELKQAVNYVLEQLKQMVPYDSASVQLREGD
                     ELYIIGGQGWGDEQEVIDLRFHIPGDNPNTIVIERGSPYYLPDAGEVYQQFRKPPHNH
                     IRSWLGIPLLVQEKVIGLLAIDSAEPNDFSEQEIMIATEFANQVAVTLENARILKETQ
                     TQAVTDPLTGISNRRGLIEAGKTEFAKTKNTNRPFSAIMLDLDYFKSINDTYGHAVGD
                     LVLREFAKRCKSCIREIDYIGRYGGEEFIILLPDAHMKASMN"
BASE COUNT          472 a          426 c          468 g          632 t
ORIGIN      
        1 gttcatgctt gccttcatat gagcatcggg tagaaggatg atgaattctt cgccgccata
       61 ccgaccgatg taatctattt cgcggataca acttttgcaa cgtttggcga actcacgtaa
      121 aacaagatca ccgactgcgt gcccataggt atcattgatg gacttgaagt aatcaagatc
      181 taacatgatt gctgaaaatg gacgatttgt gtttttagtt tttgcaaatt cggtcttgcc
      241 tgcttcgatc agccctcggc ggttgctaat gcccgtaagc ggatcggtta ctgcttgagt
      301 ttgtgtttct tttaatattc ttgcattttc aagtgtgacg gcaacttgat tggcaaactc
      361 agtggcgatc atgatctctt gttcgctaaa gtcattgggc tctgcactat caatggcaag
      421 tagcccaatc accttttcct gtacaagcag tggaatgccg agccaggaac ggatgtgatt
      481 atgtggcggt tttctgaact gttggtagac ctcaccggca tctggtaaat aatatggaga
      541 tcctctttct ataacaatgg tattggggtt atctcctgga atatggaaac gcaggtcaat
      601 gacttcctgc tcatctcccc agccctgacc accgataata tagagttcat ctccttcgcg
      661 aagttggaca gacgcgctat cgtacggcac catttgctta agctgttcaa gaacataatt
      721 gacggcttgt tttagctcgg tctgctcggc aacagcaagc cctaccttgc ggagattttc
      781 agcggtgtct gcccgttttc gggcttcatc catggcttga aatttttcca acgctaatgc
      841 gatgagagaa gctgcttgct cacagatttg tacttcttcg tcagtaaatt gatgaggagt
      901 ctcaaatgaa acgagaacgg cacccagttt ttgatctaat gcaattaagg gcagaatcaa
      961 aagtgacttt gccgggaacc ggctaactac cgaaggcgag gcataaggcg tattgtaaac
     1021 atcctcaaca ataagcgttt tttctaattc aaggctcgat tgtgtaaacg ttttttcatc
     1081 ttctttggca gaggtgcgag cataggtttc tttgccagtt ccatacgcag cgaccggaac
     1141 aacatgtttc gttacatcat tccacaaggt gaggtaacac ccatctgctt taattaactt
     1201 tgctaggcgt tctgctgata cttcagccat ctcatttaag tttgctgcct ggagggatac
     1261 ggttgcaata tcttgtaaca accccaaagc aagttgacga cgattaagtt gatgaacagt
     1321 ttgtgtacgt gtctctgctg cggcgaggat attggctgct gtttggaagg cttccaactc
     1381 catatttgtc cattggcgct cacgacgatt ttcatcaaat gagatggatg cccagggttc
     1441 accatttacg atgactggga tggtgagttg agagattggg ttggatacat ttccccaatc
     1501 ttgagctttc cattcatatt ttgtgttatg ggtatgttcg attgtgacaa cgaccttgct
     1561 gacatctgct gcttgcccta ggttttccaa cacgctgaca atatgttgtt cccagttatc
     1621 tgttttgagg aactgttctg ctgccttact gattgcagac ataatcaaac cgtggcggct
     1681 aagttcttgt tccttttctt tgcggagcgt aatgtcatgc agggtaatga cgatgtctga
     1741 ggtgttttga tttgtacctg tgatcggtga aatggagact tcgtacgtct tttcttcttt
     1801 gccttcttga atttggattt cgttatgcgg tccgctttgc tttagatagg gaatcaacaa
     1861 gttggcaagg tgcccggcta catgattaaa aggttgacct atgactcgat cttcggtgag
     1921 gttgaatagt ttttcagtga cgtgatttac atagaggact cttttttcgc tgttcaaaac
     1981 gataacgctg tccttcat
//
LOCUS       DAOREF010000643         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45871_length_1997_cov_4.001030, whole genome shotgun sequence.
ACCESSION   DAOREF010000643 DAOREF010000000
VERSION     DAOREF010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45871_length_1997_cov_4.001030"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKI33_12500"
     CDS             complement(<1..367)
                     /locus_tag="PKI33_12500"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNM37881.1"
                     /translation="MSFEFATANRIIFGAGKLSGLGDQLKGSVKRLLLVRGRSADAIP
                     RVREILSAQGIQFNEFEVHGEPTVDVVREGVKFADGCDAVIGLGGGSVLDAGKAIAAL
                     ATNPNDVVDYLEVVGKGQPL"
     gene            complement(370..675)
                     /locus_tag="PKI33_12505"
     CDS             complement(370..675)
                     /locus_tag="PKI33_12505"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNM37882.1"
                     /translation="MNIVLVHVHVKPEFVDAFKQASLENASNSVKEEGIARFDVIQQA
                     DDPTRFILVEVYKTVEAQSAHKETAHYLTWRDTVAEMMAEPRQGVKYSNIFPADGGW"
     gene            complement(685..1827)
                     /locus_tag="PKI33_12510"
     CDS             complement(685..1827)
                     /locus_tag="PKI33_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNM37883.1"
                     /translation="MQTDLLIIGAGPFGLSLAAYAKSLGMDFLVAGKPMEFWKKNMPA
                     GMFLRSASDWSLDPTDRFSIMNYLETLGKTPKDIEPISLEFYLQYAQWFQSGAGIETI
                     SEYVTRLDQKEDGFLATFEDGKVIEAKNVAIAIGMGYFKNYPAALTDILPVGRYEHTC
                     DAVQLSEYKGRRVLILGGRQSAFEWAALLNDGGAADVHVVHRHESPKFAEADWSWVPP
                     LVDGMVADPAWFRGLPQEEKDAIVKRMWGEGRLKVEPWLEKRVMKPNTSIHQKASLLA
                     CVERSDGALDVKLDNGDAFVVDDIILATGYKVELERLSFLKNGNMFDKVEVKNGFPVL
                     DPHFQTTAKGLYFTSMPAGQDFGPFFGFTVSVRASAKIIGNALVSK"
BASE COUNT          533 a          589 c          404 g          471 t
ORIGIN      
        1 tgagcggctg ccccttcccc accacttcca aataatcaac gacatcgtta gggtttgtcg
       61 ccaatgccgc gattgccttg cctgcatcca gcacactgcc gccgcccaaa ccgatcacgg
      121 catcgcaccc atcagcaaac ttaacccctt cgcgcaccac atccaccgtc ggttcgccat
      181 gaacttcaaa ttcattaaat tggattccct gtgcagacag aatctccctc acacgcggaa
      241 tcgcatccgc tgacctgccc ctcaccaaca gcaaacgctt cacactccct ttgagctggt
      301 cgcctaaccc agaaagcttc cctgccccga agatgattcg atttgctgtt gcgaattcaa
      361 aggacatttt taccaaccgc catctgctgg aaagatattg ctgtatttca cgccctgtcg
      421 tggctctgcc atcatctccg cgacagtatc acgccacgtc aagtaatgag ccgtctcttt
      481 gtgggcagat tgagcttcaa cggtcttata cacttccaca aggataaaac gcgtcgggtc
      541 atccgcttgt tgaatgacgt caaatcgcgc aataccttct tccttcacgc tattcgacgc
      601 attttcaagg cttgcctgct tgaaggcatc cacaaattct ggtttaacat gcacatgcac
      661 aagaactatg ttcatttgaa gctcctattt agataccaag gcgtttccga tgatctttgc
      721 agaagcccgc accgaaacag tgaaaccaaa gaacggacca aaatcctgcc cggctggcat
      781 gctggtgaag tacaacccct tggctgtggt ttggaaatgc gggtcgagaa ccgggaaacc
      841 attcttcact tcgaccttat cgaacatatt gccgttcttc aagaaagaca atcgctccaa
      901 ctcgacctta tagcctgtgg caagaataat atcatccacc acgaatgcat caccgttatc
      961 cagtttcaca tccaatgcgc catcactgcg ctccacacaa gcgagcaaac tggctttctg
     1021 atggatgctc gtgttcggtt tcatcacgcg cttctctaac catggctcca ccttcaaacg
     1081 cccctcaccc cacatacgtt taacgatggc atctttttcc tcctgtggca gcccgcggaa
     1141 ccaggcggga tccgccacca taccatccac cagaggcggc acccaactcc aatccgcttc
     1201 ggcaaatttc ggtgactcgt ggcgatgcac cacatgcacg tccgccgcgc cgccatcatt
     1261 tagcaacgcc gcccattcaa aagcactttg cctgccgccc aagatcaaca cccgtctgcc
     1321 tttatattca gaaagttgca cggcatcaca ggtgtgttca tatctaccaa caggcaaaat
     1381 gtctgttaac gcggcgggat aattcttgaa atagcccatg ccaatggcaa tggcaacgtt
     1441 tttggcttca atgacctttc catcttcaaa ggtcgcaagg aaaccatctt ccttttggtc
     1501 taagcgtgtg acatattcag agatggtctc gattcccgct ccagattgga accactgagc
     1561 gtactgtaaa tagaactcaa gagagatcgg ctcaatatct ttcggagtct tacctaatgt
     1621 ttccagataa ttcataatgg aaaaacgatc ggttgggtcc agcgaccagt ctgacgcaga
     1681 gcgtaggaac atgcccgcgg gcatgttctt cttccaaaat tccataggtt tgcctgcaac
     1741 gagaaagtcc atgcctaaag atttggcata tgccgccaaa gacaaaccaa aagggcccgc
     1801 accaatgata agtaaatctg tttgcattcc actctcctta gttaacgaaa attccaagcc
     1861 aaaactgata tagcccaaag ccgaacaaga tgatgcagga aagcccaagt aaaacacgat
     1921 tcaacctggg tcccaaacga gatgcggctc cgaagacaat gatcattgcg atcaggcaaa
     1981 taataaaggt cgtataa
//
LOCUS       DAOREF010000644         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_45880_length_1997_cov_3.397013, whole genome shotgun sequence.
ACCESSION   DAOREF010000644 DAOREF010000000
VERSION     DAOREF010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45880_length_1997_cov_3.397013"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKI33_12515"
     CDS             complement(<1..442)
                     /locus_tag="PKI33_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HNM37884.1"
                     /translation="MLTELGKRARLASRKLARLATAQKNAALHTLADALVADQKTILA
                     ANVSDVESAKAAGLAPAMIDRLMLNEARLADIAHNVRLVADLPDPVGEIFEESTQPNG
                     LHIRKQRVPLGVLGVIYEARPNVTVDVAGIASKAETPPFYAAAVK"
     gene            complement(454..1551)
                     /gene="proB"
                     /locus_tag="PKI33_12520"
     CDS             complement(454..1551)
                     /gene="proB"
                     /locus_tag="PKI33_12520"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNM37885.1"
                     /translation="MLIVVKLGTSTLTSGTPKISPPLLVELARQIADLQQAGHKIILV
                     SSGAIAAGREKLGYPQLPKGLPAKQMLAAIGQLRLMAIYEQIFGLYGRTIAQVLLTRA
                     DLADRRRYLNARSTLEALLAHGTLPIINENDTVATEEIRVGDNDNLSALVANVVEADL
                     LVLLTDQQGLFTADPRNNPDAKLVQEINTPEIPQEMWEAAGGSGTKLGTGGMVTKLQA
                     ADLARRSGTTVVIAQGTEPNVLSRLANNERIGTRFTPVLSVPESRKRYILSGRLSPGQ
                     ISVNEGAASALKKGGSLLPVGVTQISGDFDRGDTVRVLDSSGRELARGISTYSSADLA
                     RLRGHHSDEIESLLGYDYGDEVIHRNDLVIL"
     gene            1718..>1997
                     /locus_tag="PKI33_12525"
     CDS             1718..>1997
                     /locus_tag="PKI33_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM37886.1"
                     /translation="MALISLQEVRIGFGGHPLLDNVSFQIERGERVGLLGRNGMGKST
                     LLKMLNGDIEPHDGIIARQQNVRTAYLPQEVPQDLHGRIADIIASGLID"
BASE COUNT          444 a          485 c          567 g          501 t
ORIGIN      
        1 gtttcactgc cgccgcgtaa aatggcggcg tttccgcttt tgatgcgatg cctgccacat
       61 ccacggtgac gttggggcgc gcttcgtaaa tgacacccag tacgcccaat ggcacacgtt
      121 gtttgcggat gtggagtccg tttggttggg tggattcttc gaatatctca ccgacagggt
      181 caggcaggtc tgcgacgagg cgtacattat gggcaatgtc tgctaggcgg gcttcgttca
      241 acatcaagcg gtcgatcatg gcgggggcaa gccctgcggc tttggcagac tcgacatcac
      301 tcacgtttgc ggcgaggatt gttttctggt cagcgaccaa ggcgtcggct agggtgtgaa
      361 gcgccgcgtt cttttgcgcg gtcgccaggc gggcaagttt tcgtgaggca aggcgggcgc
      421 gttttccgag ttcagtcagc atgaagagtc cttttacaaa atcactaaat cgttgcgatg
      481 aatcacttca tcgccatagt catatccaag cagggattcg atctcatcgg aatgatgtcc
      541 acgcaggcgg gcgaggtctg cgctggaata agtgctaatg ccgcgtgcga gttcacgtcc
      601 acttgagtcg agaacgcgga cggtatcgcc acggtcaaag tctccgctaa tttgtgtgac
      661 cccaaccggc aaaagactgc cgcctttttt gagtgccgaa gctgcacctt cgttgacgct
      721 aatctgcccc ggcgaaagcc taccggaaag gatatagcgt ttgcgactct caggcaccga
      781 gaggacgggc gtaaagcgtg tgccaatgcg ttcattattt gcaagacgtg aaagcacatt
      841 gggttctgtg ccctgtgcaa tgacaactgt ggtgccagaa cgacgggcga ggtctgccgc
      901 ttgcagtttg gtaaccatgc cgccagttcc gagcttagtg ccgctgccac ctgccgcttc
      961 ccacatctct tgcggaattt caggcgtatt tatttcttga acaagttttg catcggggtt
     1021 gttacgtgga tccgcagtga agagaccttg ttggtcggtg aggagaacga gcaagtccgc
     1081 ttcgacgacg ttggcaacca aagcagagag attgtcattg tcacccacac gaatctcttc
     1141 tgtggcgacg gtatcatttt cgttgatgat aggcagagtg ccgtgtgcaa gtaaggcttc
     1201 aagcgtactg cgtgcattga ggtatcggcg acgatctgct aagtccgctc gagtgagtag
     1261 cacttgggca atggtgcgcc catacaaccc aaatatctgt tcgtagatcg ccatcaatct
     1321 taattgacca atggctgcca acatttgctt ggcaggcaaa cctttaggga gttgtgggta
     1381 gccaagtttt tcacgccccg ctgcaatggc acccgaagag actagaatga tcttgtgccc
     1441 agcctgttgc aagtctgcga tctggcgggc aagttccacc agcagtggcg gcgaaatttt
     1501 tggtgtgcca gaggtgagag tagaggttcc gagtttgacg acgatgagca taagaattac
     1561 acagtatcgc ataatttagt aaacaaaaaa ttcgatttct attcgtaatt ttttgtgaaa
     1621 gtttacttat atagcgataa gtctgagatt atacccgcca tgctttgcat tatcggctac
     1681 aattcgcgct catgaaatta tctcagaggt ttatggcatg gcattgatca gcttgcagga
     1741 agtccgcatt ggttttggcg ggcatccttt attagataat gtaagttttc agatcgagcg
     1801 cggcgagcgg gtcggtttgt tggggcgtaa tggcatgggc aagtccacgc tgttgaaaat
     1861 gctcaatggt gacatcgagc cgcatgatgg catcatcgcc cgtcagcaga acgtccgcac
     1921 ggcatatctt ccgcaagagg tgccgcaaga cctgcacgga cgcatcgcgg acatcatcgc
     1981 ttcgggtctc atcgaca
//
LOCUS       DAOREF010000645         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_46201_length_1990_cov_3.295090, whole genome shotgun sequence.
ACCESSION   DAOREF010000645 DAOREF010000000
VERSION     DAOREF010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46201_length_1990_cov_3.295090"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKI33_12530"
     CDS             complement(<1..535)
                     /locus_tag="PKI33_12530"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNM37887.1"
                     /translation="MKYTSLSIQTQREFPNNARTQGFGWLVRAGYMTRENQLLPLGEK
                     AIEKLKTQSTEVTFLSSLELPLISNADETFFESAIGSQEIIYCQSCGYIARRELAQTR
                     KTPFAQEEPLPIEKIATPDTNTIESLANLLNIPKEKTAKALMYTRITDGRFVFIVVRG
                     DMQLSEPKLKALVGDVRL"
     gene            complement(578..1114)
                     /locus_tag="PKI33_12535"
     CDS             complement(578..1114)
                     /locus_tag="PKI33_12535"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNM37888.1"
                     /translation="MFKRNNTPAEPVQPVQAVERITSVLGSGVIWHGSINGSGGVRIE
                     GAFEGEIALRGMLVVGETGRVTCQNVRANTVIVAGAVRGNITTQKLEIRATGRVWGDV
                     VTTAFVTEEGAFLRGQIRMEESVDLNLEPAPEATPSETAQAEVIAQTPIQIPEPAPVP
                     ASTPVSTQRLPRKKKTEE"
     gene            complement(1179..1973)
                     /locus_tag="PKI33_12540"
     CDS             complement(1179..1973)
                     /locus_tag="PKI33_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNM37889.1"
                     /translation="MGLLEGKNALIFGLANDKSIAWGIIQAFLREGANIGISYAGEIM
                     EKRVKPLAEKINCQWVEECDVTKDDQIEAVVKKAEKHFGKIDILVHSIAYAGRDELSK
                     PYHQTSREGFRVAMDISVFSFVALTNAFLPILNPGASVLCLTYGSGAVKVAPHYNVMG
                     VAKAALESSTRYLAYDLGAQKIRVNAISAGAIRTLAAAGVGGFRDMYKHFADKSPMRE
                     NVTIEDVGDAAVFLASDLSKRITGEVLYVDSGFNIMGVQMVEEKGE"
BASE COUNT          463 a          509 c          504 g          514 t
ORIGIN      
        1 ccaagcggac gtctccaacc aaagccttca acttgggttc gctcaattgc atgtcaccgc
       61 gcacgacgat gaaaacaaat ctgccatctg taatgcgggt gtacatcaac gccttggctg
      121 ttttttcttt gggaatgttc agcagatttg caagcgactc gatggtattc gtatcgggtg
      181 tggcgatctt ttcaataggc agaggttctt cttgcgcaaa tggagttttg cgagtttggg
      241 caagttctct acgggcgata tatccgcatg attggcaata gatgatctct tgtgagccaa
      301 tcgctgactc aaaaaaagtt tcgtctgcat tggagatcaa gggtaattcg agcgaactca
      361 agaacgttac ttctgttgac tgtgttttca gtttttcaat ggctttttca cccaagggca
      421 aaagctgatt ttcacgggtc atatacccgg cgcggacgag ccagccaaat ccctgtgtgc
      481 gggcattatt gggaaattca cgttgagttt gaatggaaag ggaggtgtat ttcatataca
      541 aattcccctc tctttttagg agagggggag agtgagatta ttcttctgtt ttcttcttgc
      601 ggggcaatcg ttgtgtgcta acaggagtgg atgccgggac aggtgccggc tcagggattt
      661 ggattggtgt ctgcgcgatg acttcggctt gagcggtttc cgaaggcgtg gcttcaggag
      721 caggctcgag attaaggtcg accgattctt ccatccgaat ttgaccgcgc aggaacgcgc
      781 cctcttccgt cacaaaggcg gtggtgacca cgtcacccca cacacgcccg gttgcacgga
      841 tttcaagctt ttgggtggtg atgtttccac gcaccgcgcc cgccacaatg acggtgttgg
      901 cgcgcacgtt ctggcaggtc acacgcccgg tctcgccgac cacgagcata ccgcgtaggg
      961 cgatttcgcc ttcgaatgca ccttcaatgc gtacaccgcc ggacccattg atggacccat
     1021 gccagattac gccagagccc agcaccgaag tgatgcgctc tacggcttgg acgggctgta
     1081 cgggttctgc gggagtattg tttctcttaa acatgaaggc aattttaccg ttaaaacaaa
     1141 aagggcgagc atcatgctcg cccttgaaat tagctttctt attcgccttt ttcttccacc
     1201 atttgcacac ccatgatgtt gaagcccgag tctacgtata gaacttcgcc tgtgatgcgc
     1261 ttcgacagat ctgaagccag aaagaccgcg gcatcgccaa catcttcaat ggtgacattc
     1321 tcgcgcatgg gagatttatc tgcaaagtgt ttgtacatat cacggaaacc acctacacct
     1381 gccgcagcca gggtacggat ggcgcctgct gaaattgcat tgacacggat cttctgtgca
     1441 ccgaggtcat atgctaaata gcgcgtagag gattccaagg cagccttggc gacacccatc
     1501 acattgtagt gaggggctac cttcaccgca cccgagccat aggtcaggca caagaccgaa
     1561 gcgcccgggt tcaaaatagg taggaaggca ttggtcaaag ccacaaaaga gaagaccgaa
     1621 atatccatcg ccacacgaaa gccttcgcgg ctggtctggt gatagggttt gctcaattca
     1681 tcgcgcccgg cataggcaat cgaatggacg agaatgtcga tcttgccaaa atgcttttcg
     1741 gctttcttca ccactgcttc gatttggtca tccttggtca catcacattc ttcaacccac
     1801 tggcaattaa ttttttcagc cagcggcttc acgcgttttt ccatgatttc accagcatag
     1861 ctaatgccga tgttggcgcc ctcgcgcaaa aaggcttgaa tgatacccca ggcaatggat
     1921 ttatcgtttg ccaatccgaa gatcagcgca ttttttcctt caagtaatcc catagtctct
     1981 cctcgttaaa
//
LOCUS       DAOREF010000646         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_46352_length_1987_cov_4.436335, whole genome shotgun sequence.
ACCESSION   DAOREF010000646 DAOREF010000000
VERSION     DAOREF010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46352_length_1987_cov_4.436335"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKI33_12545"
     CDS             complement(<1..562)
                     /locus_tag="PKI33_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNM37890.1"
                     /translation="MRFTTIFFDLDDTLYPSNTGLWKAIKERMNIYMRERMGFPEDQI
                     SHIREKYFLQYGTTLRGLQAHHEIDVQDFLAFVHDLPLKDYLTPNPTLRLIIASLPTR
                     NLIFTNADSAHADRVLRALELRDLFEIIVDVNAIDPYCKPMPESFQIAMQAAGETDPS
                     KCVMIDDIHRTTLAAKEAGLFSILYNE"
     gene            583..>1987
                     /gene="mutL"
                     /locus_tag="PKI33_12550"
     CDS             583..>1987
                     /gene="mutL"
                     /locus_tag="PKI33_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNM37891.1"
                     /translation="MPIRLLSSEVSSQIAAGEVVERPASVVKELTENSLDAGATTISI
                     SVADAGRTLIEVADDGHGIPANELELAAARHATSKLVQSDDLFHIQTLGFRGEALASI
                     GSVSQMTITSRVESAKEGARLRVNGGISEKVEKVGAPVGTVVRVENLFYNVPARLKFL
                     KTDTTERRAIDSLVTRYALAYPNVRFKITDGKQVTLQTAGDGDRRSILASLYGVEVAK
                     QMLEVMSSEEGMTLTGFISPVSLTRSNRKEITFFINGRWVQEISLNSALLQAYHTLLM
                     VGRYPLTALFLDMAPEDVDVNVHPTKAEVRFRAQDKIFSFVQRSVRKALLAYTPVPAV
                     SPQLWGSRSVPSEPRTVGIDWSIGHDEVSTMDDEPSATEGTTQPVESERPSVAVNGQS
                     SFSAGMPLLRLIGQIGSTYIVAEGPDGLYLIDQHAAHERVLFEKLMAQRENKSIPSQA
                     LLAPEIVTLPPQSSKILL"
BASE COUNT          453 a          462 c          568 g          504 t
ORIGIN      
        1 cctcattgta tagaatactg aacaagcccg cttctttcgc ggcgagagtg gtgcgatgga
       61 tgtcatctat catgacgcat ttggaagggt cggtctcccc ggcggcttgc atggcgatct
      121 gaaacgattc gggcatgggc ttgcagtatg ggtcaatggc gttcacgtcc acgatgatct
      181 caaagaggtc gcgcagttca agcgccctta acactctgtc ggcgtgggca gagtctgcgt
      241 tggtgaagat caggttacgg gtcggaagtg aggcgatgat caagcgtaga gtcgggttgg
      301 gtgtgaggta atctttgagc ggcaggtcat gcacgaaggc gaggaaatcc tgcacatcga
      361 tttcgtgatg ggcttgaagt ccgcgcaggg tggtgccata ttgcaaaaaa tatttttctc
      421 ggatgtgcga tatctggtct tcaggaaagc ccatgcgttc acgcatgtaa atgttcatgc
      481 gctctttgat ggctttccac aagccggtat tggatggata gagcgtatcg tcaaggtcga
      541 agaagatggt ggtgaatctc attcggcaat tataattgcg ccatgcctat tcgattgctt
      601 tcttctgaag tctcatcgca aattgccgcg ggtgaagtag tagaacgccc cgcttctgta
      661 gttaaagaat tgactgaaaa ttctttggat gcaggtgcga cgacgatctc gatctcggtt
      721 gcagatgccg ggcggactct gatcgaagtg gcagacgatg gacatggcat tcccgcaaat
      781 gaattggaac tggcagcggc tcgacacgca acctcgaagc tggttcagtc cgatgacctg
      841 tttcacatcc agaccttggg cttccgaggg gaagccttgg catccatcgg ctccgtctcg
      901 cagatgacca tcacttcacg agtggagtct gccaaagaag gtgcgcgtct gcgtgtgaat
      961 ggcggcattt cagaaaaggt ggaaaaagtt ggcgcgccag tagggacagt agtacgcgtt
     1021 gaaaatctct tttataacgt gcctgctcgt ttgaaatttt tgaaaaccga tacaaccgaa
     1081 cgccgtgcga ttgattcgtt agtgacgcgt tatgcgttgg cgtacccgaa tgtacgtttc
     1141 aagatcactg acggcaagca agtcacattg caaaccgctg gcgatggaga tcgccgctct
     1201 atcttggctt ctctatatgg tgttgaagtt gccaagcaga tgttggaagt gatgtccagt
     1261 gaagaaggca tgacactcac tggcttcatc agcccggtct cgttgacacg ttcgaatcgc
     1321 aaagaaatta ctttctttat caatggtcgc tgggtgcagg agatttctct aaactccgcc
     1381 ttgctgcaag cctatcacac gctcttgatg gtgggacgct atccgctcac cgctttgttc
     1441 ctggatatgg cacctgaaga tgtggatgtg aacgtccacc cgacgaaggc ggaagtacgt
     1501 tttcgagcgc aggataaaat ttttagcttt gtgcaacgct cggtgagaaa agcattgctg
     1561 gcgtataccc ctgtcccagc agtttcgccg cagttgtggg gttcgcgctc tgtgccttca
     1621 gaaccgcgga cggtgggaat cgattggtcg atagggcatg atgaagtttc gacgatggac
     1681 gatgagccat cagcaacgga aggcaccact caacctgttg aaagtgaacg tccatcggtt
     1741 gcggtcaatg gtcaatcatc tttctctgct gggatgccct tgctgcgcct gatcggtcag
     1801 atcggctcca cgtatattgt ggcagaaggt ccagatgggt tgtatctcat tgaccagcat
     1861 gccgcgcatg agcgggtgtt attcgaaaag ttgatggcgc aacgcgagaa taagagcatt
     1921 ccctcccaag cgttgctcgc tccagaaatc gtcactttgc cgccgcaatc gtcgaagata
     1981 ttgttgg
//
LOCUS       DAOREF010000647         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_46533_length_1983_cov_4.101141, whole genome shotgun sequence.
ACCESSION   DAOREF010000647 DAOREF010000000
VERSION     DAOREF010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46533_length_1983_cov_4.101141"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKI33_12555"
     CDS             <1..734
                     /locus_tag="PKI33_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM37892.1"
                     /translation="GDAVLLEAQDIPVGILDTVEYTTQSLSLAHGDLFVAASDGFPES
                     RNPAGEMYGYERMKNILSASRQLSSKQIAEALLNDVNTFCETHPQDDDRTIIVLKFEK
                     QNEKQIMFNDSLTVRANYEDIRQPCERLRTNLSQSGVSEEIAGLCELALQELLTNLVD
                     HAYEGNLNGQITVRMASKNGQIFLQTEDTGLPARIKMEDVHMPNPEDLAEGGYGMAII
                     RSLMDDIRYESMQGKNIWTLIKNLK"
     gene            759..1103
                     /locus_tag="PKI33_12560"
     CDS             759..1103
                     /locus_tag="PKI33_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM37893.1"
                     /translation="MSLEINSTEQVIKIAAITLKGRLDAFEAPALRKTCDAYIADEFS
                     HFVFDLTEVAMLDSAGLAVLVSVLKRARLKNGDVRLVWPREEAASRILKLTKFDQVFV
                     SIEKGEIVPRGF"
     gene            1105..1479
                     /locus_tag="PKI33_12565"
     CDS             1105..1479
                     /locus_tag="PKI33_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM37894.1"
                     /translation="MSKAKILVVDDEPNNQRILTYTLNKAGYESAAIADGENTLVWLA
                     SNTPDLAILDVSMPGMDGITLLTHIRSMPQYSRLPVIILTGSGDDDERLRAEGLGIQA
                     FLTKPASSKTVLEAVANLLPHE"
     gene            1472..>1983
                     /locus_tag="PKI33_12570"
     CDS             1472..>1983
                     /locus_tag="PKI33_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37895.1"
                     /translation="MNKLKELLRSKLASASALLMLSMTVVNAGNYIYNLIMGRWLGPS
                     LFADLSLIVTLLLVVTFLTAPIQMTAARYSAIHTADGDDKTLASLRRFIWLVALGVGL
                     TLTAFFAIFSTTLKVFFHTQSALPFVIFGAALPFGMMQAVERGMLQGRTSFKVLAISY
                     QVEMWSRLLV"
BASE COUNT          508 a          474 c          458 g          543 t
ORIGIN      
        1 atggcgatgc tgttctgtta gaagcacaag atattcccgt cggtattttg gatacggtgg
       61 agtacaccac ccaaagccta agcctggcac acggtgacct ttttgttgcc gcatcagatg
      121 gctttcccga atctcgaaac ccggcagggg agatgtatgg ttacgagcgt atgaagaata
      181 tcttatccgc ctcgcgtcaa ttgtcctcta aacaaattgc cgaagccctg ttaaacgatg
      241 taaatacttt ttgcgaaact catccacaag atgatgaccg caccattatt gttcttaaat
      301 ttgaaaaaca aaacgagaaa cagatcatgt tcaatgacag tctaactgtt cgtgccaatt
      361 atgaagatat tcgccagcca tgtgagcgct tgcgtacaaa tttaagtcag agcggggttt
      421 cagaagagat tgccggcttg tgcgaactcg ccttgcaaga actacttacg aacctggttg
      481 accatgccta cgaagggaat ttgaacgggc agatcacagt gcgtatggca agcaagaatg
      541 ggcagatttt cctgcaaacg gaagataccg gcttgcctgc tcgtataaaa atggaagatg
      601 ttcatatgcc aaaccccgaa gaccttgccg aaggcggtta tggcatggcg atcatccgtt
      661 cgttgatgga tgatattcgc tatgagtcta tgcaaggcaa gaatatctgg acgcttatca
      721 agaatttgaa ataatggaat caagagaaag aggtaactat gtcactcgaa attaattcta
      781 cagaacaagt cataaaaatt gctgccatca cactaaaagg caggctcgat gcctttgaag
      841 cccccgcgct acgaaaaact tgtgacgcat acattgccga tgaattcagc cattttgttt
      901 ttgacctgac cgaggtagcc atgctggact cggctgggtt ggcagtcctt gtcagtgttc
      961 tcaaacgggc ccgcctcaag aatggcgatg tgcgtcttgt ctggccccgc gaagaagcgg
     1021 catcccgcat actcaaactc accaagttcg atcaggtttt tgtctctatc gaaaaaggcg
     1081 agatcgtacc aagaggtttt taacatgagc aaagctaaaa ttctcgtagt ggatgatgaa
     1141 cctaacaatc agcgcatcct cacctatacc cttaataaag ccggctatga aagtgcagcc
     1201 attgctgatg gcgaaaacac gttggtttgg cttgcatcta atactcccga tcttgccatt
     1261 ctggatgtat ccatgccagg catggatgga attactttgc taacgcacat ccgctctatg
     1321 ccgcagtatt ctcgtttgcc cgtcattatc ctcacaggta gtggcgatga tgatgaacgt
     1381 ctgcgtgccg aagggctggg cattcaagct tttcttacca aaccagccag ttctaaaacg
     1441 gtcttggaag cagtagcaaa tctactccct catgaataag ctcaaagaac tacttcgctc
     1501 caaactggca tctgccagcg cgctgcttat gcttagcatg accgtcgtca atgcgggcaa
     1561 ttacatctat aaccttatta tgggcaggtg gctgggtccg tcactgtttg cagacctttc
     1621 actcattgtt actttgctgc tagttgtgac ctttctcacc gctcctattc agatgaccgc
     1681 tgcgcgttat tctgccattc ataccgccga tggagatgac aaaaccctcg cctctcttcg
     1741 acgttttatc tggctcgtcg ccttgggggt gggtctcacg ctgacagctt tctttgccat
     1801 tttttcaact acattaaagg ttttctttca tacgcaatct gctcttcctt ttgttatttt
     1861 tggcgcagca cttccttttg gcatgatgca agctgttgaa cgtggaatgc ttcaggggcg
     1921 caccagtttc aaggttttgg caatcagtta tcaagtcgag atgtggagcc gtttgttggt
     1981 cag
//
LOCUS       DAOREF010000648         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_46572_length_1982_cov_5.066943, whole genome shotgun sequence.
ACCESSION   DAOREF010000648 DAOREF010000000
VERSION     DAOREF010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46572_length_1982_cov_5.066943"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKI33_12575"
     CDS             complement(<1..281)
                     /locus_tag="PKI33_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNM37896.1"
                     /translation="MAREINFGKRKLGDGHPAYIIAEIGLNHNGDLDIAKKIIDAAVH
                     AGADAVKFQKRTPEVCTPPEQQKQMRETPWGYITYLDYRYKVEFNEEQYR"
     gene            complement(284..1510)
                     /locus_tag="PKI33_12580"
     CDS             complement(284..1510)
                     /locus_tag="PKI33_12580"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNM37897.1"
                     /translation="MTNILAIIPARGGSKGIPRKNIRSFAGYPLIAWSIAAAKRSSLV
                     TRVIVSTDDEEIAEVAREWGAETPFLRPSELAQDKTTDLPVFEHALKWLEEIEGYHPE
                     IVVQLRPTSPIRPKGMVDDAVNILLNHADADCVRGVVPAGQNPFKMWRFNSEEKPLGQ
                     LLEVPGIAEPYNAPRQILPSVYWQTGHIDAIRTSTIVNKKSLTGNIIYPLVIDPKYTV
                     DIDTLPDWAKYEAVIYSGLEVVSPGKSRRTMPKTIKLIVTDFDGVLSDGRVWVDENGK
                     ETVAANRSDSMRIRQIRELGMDVIILSSEVNPVVSMRAKKMKVEAIHGMGLDEKGEVL
                     KKYLAEKHIDPAQVVYLGNDFNDLPCFEVAGWSVAVADAYPEVVRAADHVLNTNGGFG
                     ALRELCDLILKEYKEN"
     gene            complement(1581..>1982)
                     /locus_tag="PKI33_12585"
     CDS             complement(1581..>1982)
                     /locus_tag="PKI33_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM37898.1"
                     /translation="VKQMIEQGRKGSIVNICSTYGLNGPDQRIYIKDGKRVAFKPVYY
                     TTTKAGVMGFTKYLAAYYAETEIRVNALTPGGVYNNHEEYFVKNYSAKTILGRMAKKD
                     EMNGALLFLASDASSYMTGNNVIVDGGWTAW"
BASE COUNT          481 a          534 c          432 g          535 t
ORIGIN      
        1 cgatattgtt cttcgttgaa ttccactttg tagcggtaat ccaaataagt gatatagccc
       61 cagggcgtct cacgcatttg tttctgttgt tcaggtggcg tacaaacttc aggcgtgcgt
      121 ttctggaatt tcaccgcatc cgccccggca tgcacggcag catcgataat tttcttggca
      181 atatccagat caccgttatg gttgagcccg atctcagcaa taatatatgc ggggtgtcca
      241 tcgcctaatt tacgttttcc aaaatttatt tcacgagcca ttcttaattc tccttatact
      301 ctttcaaaat taagtcacat aattcgcgca gcgccccaaa gccgccgttg gtattcaaca
      361 catggtcagc ggcacgcacc acttcagggt atgcatctgc cacggcaacc gaccagcccg
      421 caacttcaaa acaaggcagg tcattgaaat catttcccaa atacaccact tgcgccgggt
      481 caatatgttt ctcggcaaga tattttttca acacctcacc tttttcatcc aaacccatgc
      541 cgtgaatcgc ctccaccttc attttcttgg cacgcatact cacaaccgga ttcacttcag
      601 aagacaggat gatgacatcc atgcccagct cacgtatctg acgtatccgc atcgaatcgg
      661 accggtttgc tgccactgtt tccttaccat tttcatccac ccaaactcgt ccatcgctca
      721 acacgccatc gaagtcagtg acgatgagtt taatagtctt tggcatcgtc cgacgtgatt
      781 tccccggcga gaccacttcc agcccgctat aaatcaccgc ttcatatttt gcccaatcgg
      841 gcagagtatc aatatccact gtgtatttgg ggtcgatcac cagcggatag ataatattac
      901 ccgttagaga ctttttattt acgatggtcg aagtacgaat cgcatcaatg tgacccgtct
      961 gccaatacac tgacggcagt atttggcgcg gagcattata aggttcagca atgccgggta
     1021 cttccagcag ttgaccgagc ggtttttctt cgctgttgaa gcgccacatc ttgaaagggt
     1081 tttgccctgc aggcacaacc ccgcgcacac aatcggcatc ggcatggttc aagagaatat
     1141 taaccgcatc atccaccatt cctttcgggc ggatgggtga agtaggtcgc agttgtacca
     1201 cgatttcagg gtgatatcct tcgatctctt ccaaccactt gagcgcatgt tcgaacacag
     1261 gcaagtctgt cgtcttatct tgtgccaact ctgacgggcg caaaaaggga gtttcagcgc
     1321 cccattcacg agccacttct gcaatctctt catcatctgt cgagacgata acacgcgtca
     1381 ccaagctgga tcgcttcgcc gcggcaatac tccacgcaat aagcggatat cccgcaaacg
     1441 agcggatgtt cttacgcgga atgcccttcg agccgccgcg agcagggata atggcaagaa
     1501 tattggtcat aagttctcga tattcgcatt tcgatattcg acattcgtca accgaatatc
     1561 gagttctcga ttatcgtttt ttaccacgct gtccaaccac cgtcgacaat aacgttgttg
     1621 ccggtcatgt aagaagaggc atctgaagcg aggaagagca aggcgccatt catttcgtct
     1681 ttctttgcca tgcgtccaag gatagttttg gcagaataat tcttgacgaa atattcttcg
     1741 tggttgttgt aaacaccgcc gggggttaat gcattgacgc ggatctccgt ctcagcatag
     1801 taagcggcaa ggtacttggt aaaacccatc acaccggctt tggtggtggt gtaatatacc
     1861 ggcttgaaag ccacacgttt gccatctttg atgtaaatgc gctgatccgg gccgttcaag
     1921 ccgtaggtgg agcagatatt gacaatgctg ccttttctgc cctgctcaat catttgctta
     1981 ac
//
LOCUS       DAOREF010000649         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_46806_length_1977_cov_6.317898, whole genome shotgun sequence.
ACCESSION   DAOREF010000649 DAOREF010000000
VERSION     DAOREF010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46806_length_1977_cov_6.317898"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1443)
                     /gene="ltrA"
                     /locus_tag="PKI33_12590"
     CDS             complement(133..1443)
                     /gene="ltrA"
                     /locus_tag="PKI33_12590"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNM37899.1"
                     /translation="MTQLSQDRRKSSGQSKPFAISKWDVMTAFEKVKANQGGAGIDGV
                     TIEDFEKDLKKNLYKIWNRMSSGSYFPPPVAAVSIPKKAGGERILGIPTVSDRVAQMV
                     VRDKLEIMVEPHFLEDSYGYRLGRSAHDAIGITRERCWKYDWVLEFDIKGLFDNIRHD
                     LLMKAVRKHVQHAEQRQGCNYEWVILYIERWLVAPLQKADGTRTEREIGTPQGGVVSP
                     VLANLFLHYVFDKWMEKHYPDNPWCRYADDGLVHARNRNKAEILKRELEARFIACGLE
                     MHPTKTKIVYCKDKSRKGSAPNTQFDFLGYTFRARSNKCKRTGEIYERFLPAVSTTAQ
                     KAMRRKVKELKVRQKTQYSLEQLSKWLNPMLRGWINYYGKFRRSELDSVFRHFNKTLV
                     RWARRKYKQLKRHKGKAVAFFDKLSVREPKMFAHWQFGSARSFA"
BASE COUNT          459 a          551 c          358 g          609 t
ORIGIN      
        1 gaaacgagtg ctccttgcag atccagcagt tgcaatagct cggggatcgc cgtgaggtga
       61 gtagaccgcc ggaacctccc cagcagcctc tcgcaaaacg gaacgtgaac ctctcgattc
      121 attccgcttc catcaagcaa aactccttgc agaaccaaac tgccaatggg caaacatttt
      181 aggctctcga acggatagct tgtcaaagaa tgccaccgcc ttacctttgt gccgtttgag
      241 ctgcttatat ttccttctcg cccaacgcac taacgtttta ttgaagtgtc tgaagaccga
      301 gtctaattcg cttcgtctga attttccgta gtagttgatc caaccccgta gcattggatt
      361 taaccacttt gacagttgtt ctaaactgta ttgggtcttc tgacgcactt tcaactcttt
      421 tacttttcta cgcatcgctt tctgggctgt tgtgcttacc gctggcaaaa atcgctcgta
      481 gatctctccc gttcgcttac atttattcga tctggctctg aatgtataac ccagaaagtc
      541 aaactgtgta ttaggtgctg aacctttgcg gctcttatcc ttgcaataca caatttttgt
      601 cttcgtggga tgcatttcca gaccacatgc aataaagcga gcttcgagtt ctctcttcag
      661 tatttctgcc ttattccggt ttcttgcatg aaccaaccca tcatctgcat agcgacacca
      721 tggattgtct ggatagtgtt tttccatcca tttgtcgaat acatagtgca gaaataagtt
      781 cgcaagcaca ggactaacta caccgccttg tggggtccct atttctctct ctgttctcgt
      841 tccatctgct ttctgtaatg gcgcgaccag ccacctttca atatacagaa tgacccattc
      901 ataattgcag ccttgtcttt gctcagcatg ctgaacgtgt tttcgcactg ctttcattaa
      961 taaatcatgt cggatattgt caaacagtcc cttgatatca aattccagta cccaatcgta
     1021 cttccagcat cgttctcttg ttattcctat cgcatcatgg gctgatcgac ccaatcgata
     1081 cccataagaa tcctccagaa aatgcggttc aaccatgatt tcaagtttgt ctcgaacgac
     1141 catctgtgcc actcgatcac taactgttgg gatacctaaa atcctttctc ctcccgcttt
     1201 cttcggtata ctcaccgcag ccaccggtgg tggaaaatag gaccctgatg acatcctgtt
     1261 ccatatttta taaagattct ttttcagatc tttttcaaaa tcctcaatcg tcacgccatc
     1321 gatcccggct ccaccttggt tggctttcac cttctcgaaa gcagtcatta catcccattt
     1381 tgatattgca aaaggtttgg actgtccaga tgacttcctc ctatcttgcg atagttgagt
     1441 catcctttca tcctgcttga tgcaatccct tcgctccaca ctcattacaa gtgcttcctc
     1501 actactacga attgctccgc cccagtatca cgcattggta ctctcacgct cataatttca
     1561 gttattcgca tttcttcctt cacatcgtga tgactggttc ccgtggttcc cacaaagagc
     1621 ctagtgcaag gtcatgccca ctttacgccg agcaccgcac acccagcatt cggactttac
     1681 cggtgtactt gtcctgaaat aacgaaacga tttcagtttt gatgctgccc taagttaacg
     1741 acgcgtctac atgggttcgg ggttactcat ctccttgcac cctacctcgc cagaataaac
     1801 ttctgatttt ctttcaacgc ttctgaccat tactcttaat aacagccact tgaaagtggt
     1861 ttagagccag cttccgacag cagactctgg agggtctacc tccatctctt catgagctta
     1921 tgctcattca cttacctact ttccttagcc agtaagtttc agtgcccacg gcacact
//
LOCUS       DAOREF010000650         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_46812_length_1977_cov_4.147242, whole genome shotgun sequence.
ACCESSION   DAOREF010000650 DAOREF010000000
VERSION     DAOREF010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46812_length_1977_cov_4.147242"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PKI33_12595"
     CDS             <1..1199
                     /locus_tag="PKI33_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37900.1"
                     /translation="LPSHALNDAPIEKPGANDINAAAIISEHGSALEKKIESAFGADE
                     GLVKIAGRWFPRALLVDINEGQLNLVEAVLDMAGGEPLTTADLMKDTELPTGVNPKLV
                     EFSLNYAMQYDDRFDEVGPAGQVLWCLRRLEPDFVRDVPPPLRYMDIPHDRSFLSPEM
                     LALEAQLDDELTETDAADRDDVSSVTITLIYPHLRAGTLPVSPRTRKLFPTAYETPRV
                     RFTLVDGKTKQRIPAWVVLEHGYVFGLREWYKAHQLIPGSLVQVRKGENPGEVIVEAK
                     SQRSAKDWVRTVMVGSDGGFVFATLKQPITAEFNDRMVVHVPDFKALDPIWEKKRSFE
                     DLLLMVMRELAKSNPQGHVHAQELYAGVNLVRRVPPAPIFAALASNPVFKHVGDLHFR
                     LDEEVN"
     gene            1199..1633
                     /locus_tag="PKI33_12600"
     CDS             1199..1633
                     /locus_tag="PKI33_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37901.1"
                     /translation="MSLLKPTVKTPFHIDFEWWKQNEGDWHIFLRSFLCAEHQEAFAN
                     VEEGEMIDWIDPQTAEVKPVDGVQHALISHCAQLPDFVNQRTAIVEAIFRLFLANGNI
                     PMSAEDLSKKLGKPADTILRTIAGPRVYRGLRPMLNQPTETM"
     gene            1658..1730
                     /locus_tag="PKI33_12605"
     tRNA            1658..1730
                     /locus_tag="PKI33_12605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1691..1693,aa:Arg,seq:tct)
     gene            1765..>1977
                     /locus_tag="PKI33_12610"
     CDS             1765..>1977
                     /locus_tag="PKI33_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37902.1"
                     /translation="MPTVIRKTRETLMEVRRGVLHAVSWQVKSSVWDPPTDLYETEEA
                     YIVRMEIAGMREENFEVSLLNDTLYIT"
BASE COUNT          480 a          464 c          515 g          518 t
ORIGIN      
        1 atttgccttc acatgctttg aacgatgctc cgatcgaaaa gccgggtgca aatgacatta
       61 atgcggctgc aattattagt gaacatggct ctgctcttga gaagaaaatt gagtctgcat
      121 ttggggctga tgaaggattg gtgaaaattg caggacgttg gttcccacgc gctttgttag
      181 tggatatcaa cgaaggtcaa ttaaatcttg tagaagctgt tttagatatg gcaggcggtg
      241 agcctctaac cactgctgat ttgatgaaag atactgaact gccaactggc gtaaacccta
      301 agctggttga gttttctttg aactatgcga tgcagtacga tgatcgcttt gacgaagtgg
      361 gtcccgccgg gcaggtactt tggtgcttac gccgcctcga accggacttt gtgcgggatg
      421 tccctccgcc gcttcgctac atggacatcc ctcatgaccg tagcttctta tctcctgaaa
      481 tgctcgcgtt ggaagctcaa ctcgacgatg agctaactga gacagatgct gcagatcgcg
      541 atgacgtttc ttccgttacc atcaccttaa tctacccaca ccttcgtgcc gggacgttac
      601 ccgtctcacc gcggacccgc aaactgttcc caaccgcgta tgagacgcca cgtgtgcgtt
      661 ttacccttgt agacggtaag accaaacaac gcattccggc ttgggttgtt ttggaacacg
      721 gctatgtctt tggtttacgt gaatggtaca aggcgcatca attaatccct ggttcgttgg
      781 ttcaggtgcg caagggtgag aaccccggtg aagtgatcgt ggaagcaaaa agccagcgtt
      841 ctgcaaagga ttgggtccgc accgtaatgg ttggttcaga tggcggcttt gtctttgcaa
      901 cccttaaaca gcccatcact gcggaattta atgaccgtat ggtggtgcat gtccctgatt
      961 tcaaggcgct cgacccgatc tgggaaaaga agcgttcttt tgaggattta ctcttaatgg
     1021 tcatgcgtga attagctaaa tcaaacccgc aaggacacgt ccatgcgcag gaattgtatg
     1081 ctggcgtgaa tcttgttcgc cgtgtgccgc ctgcaccgat ctttgctgct ttggcatcga
     1141 accccgtttt caagcatgtt ggcgatttgc acttccgcct cgatgaagag gtgaactgat
     1201 gagtctctta aagccgacag taaaaacacc gtttcatatt gattttgaat ggtggaagca
     1261 aaatgaaggg gactggcata ttttcttgcg atctttcctt tgtgccgaac atcaagaagc
     1321 atttgccaat gtagaagaag gcgaaatgat cgactggatc gacccgcaaa ctgccgaggt
     1381 taaaccggtg gatggggttc agcatgcttt gatcagtcac tgtgctcagt tgcccgattt
     1441 cgtcaatcag cggacggcga ttgttgaggc aattttccgc ttgttccttg ctaacggaaa
     1501 tattcccatg tctgctgaag atttaagcaa gaaattgggg aaacctgccg acaccatcct
     1561 gagaactatt gcgggtccgc gggtgtaccg tggtttacgt cccatgttga accagcccac
     1621 tgagaccatg tagtcgggta aaattaaagc aacacaagcc ctcgtagctc aatggacaga
     1681 gcagcagcct tctaagctgt tggttgggag ttcgattctc tccgggggcg ctcgaaagct
     1741 ggcaagatat taaggagtcg tcctatgcct actgtgattc gaaaaacacg cgaaacactt
     1801 atggaggtca ggcgcggcgt cctgcatgca gtgagttggc aggtcaaatc aagcgtctgg
     1861 gatcctccaa ctgacctcta tgagaccgaa gaagcctata ttgtgcgcat ggaaattgca
     1921 ggaatgcgcg aagagaactt tgaggtttca ctcttaaacg atacgcttta catcact
//
LOCUS       DAOREF010000651         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_46896_length_1976_cov_1.684539, whole genome shotgun sequence.
ACCESSION   DAOREF010000651 DAOREF010000000
VERSION     DAOREF010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46896_length_1976_cov_1.684539"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1976
                     /gene="mutS"
                     /locus_tag="PKI33_12615"
     CDS             <1..>1976
                     /gene="mutS"
                     /locus_tag="PKI33_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNM37903.1"
                     /translation="VASVAYTDVTTGEFAVTELPIEALRAELTRLHPAEILYPDNQKL
                     PDGIIGHTTHVPAWKFEPGKCNDVLLAQFKASTLDGFGLKANSLSVRAAGAIIQYLKE
                     TQPDALNLLNSLRSYSLNEFMTLDASTRRNLELDETLRGERKGSLLGTLDATITPMGK
                     RMIHQWVSQPLLNVERINKRQVGVEYFVQNGMVRAQLRNALKPIADLERLINRVIAGH
                     AQPRDLVAMRSTLSNLPNIIEALKEKGKKTDIQLPAIDLLGEQLSLLQNALDDDPPAT
                     LQNTGVIRPGYSAELDGVIDASKHAREWIANLEAVEREKTGIKTLKVGYNKVFGYYIE
                     ISRGAADKAPEHYIRKQTLVNAERFITPEMKEYETLVLNAEERIKEIETRLFKEICGD
                     LTKSANKILATARSIAELDVLSALGEVAALNGYTKPQVHEGSELEIAEGRHPVVEQYL
                     KSDRYIPNDVIFEKGEIVRVITGPNMSGKSTYLRQTALIVLMAQMGSFVPAGNAKIGL
                     VDRIFTRIGAQDEIHAGQSTFMVEMVETANILHHATNRSLLILDEIGRGTSTYDGLSI
                     AWGIIEYVHNHPQLRAKTLFATHYHELTQLAELLPGVRNYNVAVSEADGKVVFLHKII
                     AGGADKSYGIHVAQLAGLPSPVIGRANEIMTELE"
BASE COUNT          572 a          483 c          445 g          476 t
ORIGIN      
        1 cagttgcctc tgttgcttat accgacgtca ccactggaga attcgccgtt accgaactcc
       61 ccatcgaagc tctacgcgca gaactaaccc gccttcaccc cgccgaaatt ctttacccag
      121 ataatcaaaa acttcctgat ggaattattg ggcataccac tcacgtccct gcctggaagt
      181 ttgagcccgg caaatgcaat gacgttttgc tggctcagtt caaagcctct acattagatg
      241 gatttggctt gaaagcgaac agcctctctg tgcgtgcagc tggagctatt attcaatatc
      301 tcaaagaaac tcagccagat gcgctcaatc tgttgaactc gctccgctct tatagcctaa
      361 acgagttcat gaccttggat gcttccactc gccgtaatct cgaactagat gaaacgttac
      421 gaggcgaacg caaaggctct cttctcggca ctcttgacgc cacgatcacc cccatgggca
      481 aacgcatgat ccaccaatgg gtaagccaac ctttgctcaa tgtggagaga attaacaagc
      541 gccaagttgg tgtggaatat tttgttcaga acggcatggt ccgcgcgcaa ttgcgaaatg
      601 cactaaaacc catcgcagac cttgagcgcc taattaatcg tgtgattgcg gggcatgctc
      661 aaccacgaga tctagtagcc atgcggtcaa cactcagtaa tttaccaaac attattgaag
      721 cattaaaaga aaaaggaaag aaaacagata ttcagttgcc tgcaattgac ctattaggtg
      781 aacaattatc tctcttgcaa aatgcattgg atgatgaccc acctgccaca ctacagaaca
      841 ccggtgtgat tcgcccaggg tattccgctg aacttgatgg cgttatagac gcctccaaac
      901 acgcccgcga gtggattgcc aatctggaag ctgtcgaacg agaaaagacg ggcatcaaga
      961 ctctcaaagt aggctacaac aaagtctttg gctattacat agaaatctcg cgcggagcag
     1021 cagacaaagc accagaacat tacatccgca aacagacctt ggtcaacgcc gaacgcttca
     1081 tcacgcctga gatgaaagaa tatgaaacgc tagtcttaaa tgccgaagaa cgcatcaaag
     1141 aaattgaaac ccgtttattc aaagaaattt gtggcgacct gacaaagtcc gcaaacaaaa
     1201 ttttggcaac ggcacgctct atagccgagc tggatgtttt atccgctctc ggcgaagtcg
     1261 cagccttgaa tgggtatacc aagcctcaag tccatgaggg aagcgaactt gaaattgctg
     1321 aaggaaggca tcctgttgtt gagcagtatc taaaatctga ccggtacatc ccgaatgatg
     1381 tcatcttcga aaaaggtgag attgtgcggg tgataacggg accaaacatg agcggtaaat
     1441 ccacatatct gcggcagact gctcttattg tacttatggc acaaatgggc tcatttgtgc
     1501 cagcaggcaa tgcaaagatc gggctggttg atcgcatctt tactcgcatc ggcgcacaag
     1561 atgaaattca cgccgggcag tccactttca tggttgaaat ggttgagacg gcaaacatct
     1621 tgcatcatgc tacaaatcgt agtttattga ttttggatga gatcgggcgc ggcacatcca
     1681 cttatgatgg cttgtcaatt gcatggggca tcattgaata cgtacacaat catccgcaac
     1741 ttagggcaaa gacattattt gccacacatt accatgagct aacacaacta gcagaattac
     1801 ttcctggtgt gcgcaattac aatgttgccg tcagtgaagc agatggcaaa gtcgtttttc
     1861 tacacaagat tattgcaggc ggagctgata aatcatacgg cattcatgtc gctcaactgg
     1921 ctgggttacc ttcccctgtt attggtcgtg caaatgaaat catgacagaa ttagag
//
LOCUS       DAOREF010000652         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47021_length_1972_cov_4.467919, whole genome shotgun sequence.
ACCESSION   DAOREF010000652 DAOREF010000000
VERSION     DAOREF010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47021_length_1972_cov_4.467919"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..430)
                     /locus_tag="PKI33_12620"
     CDS             complement(182..430)
                     /locus_tag="PKI33_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37904.1"
                     /translation="MTESSPKIVFNPEGNGPGDACVPNISPAERKKRMDFGILQLVIT
                     FGILAAMLYFGADKLWRLPLFAMFSSGAVSIFQALDKT"
     gene            complement(570..1487)
                     /locus_tag="PKI33_12625"
     CDS             complement(570..1487)
                     /locus_tag="PKI33_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM37905.1"
                     /translation="MSTQTNSPAKQEYSVHPATRMGHVALTVANLENQILFYEKAIGL
                     KLHWREGNKAGLGAGGADLLQLTEEPNLKRYRGGTGLYHFAIVFPNRRELAIAMARLF
                     ALKVPNSPTDHVMTKTTYLSDPEGNGIELYCESPEDGSWTIKDGNYEARWADGRWSDG
                     REALDVDALFTHLKEDDKLDAPVPAETRMGHVHLHVRDIPEAVDFYHGVLGFDVMGVS
                     KGIGMAFVSAGGYHHHIGLNTWNGRGAPPPPPDAVGLRFMTVDYPNPSTLAEVTARVE
                     AAGIPYNKTDDGYLLHDPSQNGVLLRSVG"
     gene            complement(1568..>1972)
                     /locus_tag="PKI33_12630"
     CDS             complement(1568..>1972)
                     /locus_tag="PKI33_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNM37906.1"
                     /translation="KSGRYKAVRPRVLALDRKTQADEIRKLRTIPDYIVGSVHAITEA
                     GEVLTASGTGSQISSYAYGAGKVIWVVGTQKIVSDLNEGFRRIKEYSHPLEDARMQEA
                     YGVNSSLNKILVLSRDMPGRITIVLVKEELGF"
BASE COUNT          432 a          473 c          554 g          513 t
ORIGIN      
        1 gcagagcgtt ttattgggtt catggaatta gcatgatcaa caaggttatg cctagtccaa
       61 ccagtgtccc tttgaggacg actttcattg cctggcgttt gatcttggcg tcttcggctg
      121 agtctttgac ccgttcatat ttatctgagg tgcggcgtac actacgcgcg gcgagggcta
      181 ctcaggtttt gtcgagggct tggaagatgg aaacggcgcc agaggagaac atggcgaaaa
      241 gcggcaaacg ccagagcttg tccgcgccga agtagagcat ggcggcaagg atgccaaagg
      301 taatcacaag ctggaggatg ccgaagtcca tgcgtttctt gcgctcggcg gggctgatgt
      361 tcggaacaca ggcgtcgccg ggtccgttac cttcgggatt gaacacaatt ttaggtgatg
      421 attcggtcat agtttatttt ctctaaggcg tagagacgtt ttgtgtgttg ggattcatac
      481 gggcgttttg ccacgcttaa taaggaatgg attagaaaca aaaacgccgt agagatttct
      541 ctacggcgtt tttgttgatt tctatagaat tatccaaccg agcggagcaa gacgccattc
      601 tgtgatgggt catgcagcaa gtaaccatcg tcggtcttgt tgtagggaat gccagctgct
      661 tctacgcgag cggtcacttc ggcgagtgtg gaaggattcg ggtagtccac ggtcatgaag
      721 cgcagtccca ccgcatcggg cggaggagga ggcgcgccgc gtccgttcca ggtgttgaga
      781 ccgatatggt ggtggtatcc gcctgctgag acgaatgcca tgccaatccc tttggaaaca
      841 cccatcacat cgaagccgag cacaccgtga taaaaatcga cagcttcggg aatgtcgcgt
      901 acatgcaagt ggacatgtcc catgcgggtt tcagcaggga cgggggcatc aagcttgtca
      961 tcttctttga gatgagtgaa gagcgcatcg acgtcgagtg cttcgcgtcc atcgctccag
     1021 cgtccgtctg cccagcgggc ttcatagttg ccgtctttga tcgtccacga accatcttcg
     1081 ggcgattcgc aatacagttc gatcccgtta ccttcagggt cactgaggta ggtggtcttg
     1141 gtcatgacat ggtcggtggg tgagttggga actttcaacg caaacaaacg cgccatggca
     1201 atcgcaagct cgcggcgatt gggaaatacg atggcaaagt gatacaagcc cgtgccgcca
     1261 cgatagcgtt tgagattcgg ttcttcagta agttgaagta gatccgcgcc tccggctccg
     1321 agaccggctt tattgccttc gcgccagtgc agtttcaacc cgatggcttt ttcataaaag
     1381 agaatctgat tctccaaatt ggcaacggtg agagcgacgt gtcccattct ggtggcgggg
     1441 tgaaccgagt attcttgctt tgcgggggag tttgtttgag ttgacatata tacctttatc
     1501 tttcgtccat gattctaagc ctatgtacta gatgacttaa acacggaaat actttctttg
     1561 gttcgggcta aaacccaagt tcttctttca ccagaacaat ggtgatgcgc cctggcatat
     1621 cgcggcttag aaccaatatt ttgttgagcg aactgttgac gccataggct tcttgcatgc
     1681 gggcatcttc aagcggatga ctgtattctt tgatgcggcg gaagccttcg tttagatcgc
     1741 tgacaatctt ttgtgttccg accacccaaa tcactttccc tgcgccatag gcataggagg
     1801 aaatttggct gcctgtgcct gaagccgtaa gcacttcacc tgcttctgta atcgcgtgga
     1861 cgctgccaac aatgtaatct ggaatcgtgc gtagcttacg gatttcgtct gcttgtgtct
     1921 tacggtctag cgccagcaca cgcgggcgga ccgccttata gcgcccggat tt
//
LOCUS       DAOREF010000653         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47497_length_1962_cov_2.995281, whole genome shotgun sequence.
ACCESSION   DAOREF010000653 DAOREF010000000
VERSION     DAOREF010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47497_length_1962_cov_2.995281"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PKI33_12635"
     CDS             <1..1027
                     /locus_tag="PKI33_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNM37907.1"
                     /translation="QASKYFRVKPDPNYRQSLREESKQMLNEARHIEEQVVSSILNGA
                     RVVCATATGLDPERLRGNRFEWVIMDEASQSTEPAAWIALQYGERLVLAGDPCQLPPT
                     VLSNEAARGGFNISMMERMLRLQTEASGKQPDLSRLLSVQYRMHEDIMRFSSDVFYEG
                     QLQADEAVRTALLADLPHVQAGTLTTSAIHFIDTAGASYDEEPEPDGESRMNPLEAEL
                     VVKKVNELLAQGVAAEQVAVISPYSAQVKLLRQKLNQTLEIDSVDGFQGREKDVIVVS
                     LVRSNPEGEVGFLADTRRMNVALTRAKRKLVVIGDSATITAHPFYEKLVKYFEGIGAY
                     HSVWEEG"
     gene            1194..1706
                     /locus_tag="PKI33_12640"
     CDS             1194..1706
                     /locus_tag="PKI33_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37908.1"
                     /translation="MAKVKKNPVIQGISGKIGNLVFKQLPDGSTIVTAKQVLHNKKWS
                     EGQVAHQARMREAAAYGREAQHHPVYVALAENSTLTGYNWAFGDRMKPPVIHKIERAE
                     GLVRVRASDNVGVVRVEVQVLDEKGAVQARGEAVQTSKEVWEFATELAGKVVVRVWDL
                     ARNVVEGSLE"
     gene            complement(1783..>1962)
                     /locus_tag="PKI33_12645"
     CDS             complement(1783..>1962)
                     /locus_tag="PKI33_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNM37909.1"
                     /translation="QKRVTPHTFRHSFATHLLEAGYDIRTVQELLGHKDVKTTMIYTH
                     VLQRGANAVKSPLDV"
BASE COUNT          470 a          382 c          698 g          412 t
ORIGIN      
        1 gcaagcttcc aagtatttcc gcgtcaagcc agacccgaac tatcgccagt ctttacgtga
       61 agaatcgaag cagatgctga atgaagccag acacattgaa gagcaggtgg tttcttctat
      121 tttgaacggg gcgcgggtgg tatgtgccac cgccaccggc ttagaccctg aacgcttgcg
      181 cggcaatcgc tttgagtggg tgatcatgga tgaagccagc caaagcaccg aaccggcggc
      241 gtggatcgct ttgcaatacg gcgagcggtt ggtgttggcg ggcgacccct gccaactgcc
      301 ccccactgtg ctttcgaatg aagcagcgcg aggcgggttc aacattagca tgatggagcg
      361 catgctcagg cttcaaacag aagcgagcgg gaaacagccc gatctgagcc ggctcttgag
      421 cgtgcaatat cgcatgcatg aagatattat gcgcttctct tcggatgttt tctacgaggg
      481 gcaattacaa gccgacgaag cggttcgcac ggcgctctta gcagacctgc cgcacgtgca
      541 ggcaggtacg ctgacaacga gtgcgattca cttcattgat accgccggtg ccagctacga
      601 cgaagaaccc gagccagatg gcgagagccg catgaatccg ctggaggcag agttggtagt
      661 gaaaaaggta aacgagttgt tggcgcaggg cgttgctgcc gagcaggtgg ctgtgatctc
      721 gccgtattcg gcacaggtga agttgttgcg gcagaaactg aaccagacgc ttgagatcga
      781 cagtgtggat gggtttcagg ggcgtgagaa agatgtgatt gtggtgagcc tggtgcgctc
      841 gaaccccgaa ggtgaggtgg gctttctggc agatacgcgg cgcatgaatg tggcattgac
      901 gcgcgccaaa cgcaagctgg tggtgatcgg cgatagcgcc acgattacgg cgcatccgtt
      961 ctacgagaag ctggtgaaat actttgaagg gatcggggcg tatcactcgg tgtgggagga
     1021 gggttgagga aaggttggga gatgattggg ggttgattgg agattgattg gagctataat
     1081 ggattaagcc tacagggacc ttataggaag cttatagaaa ccttatagga accctataga
     1141 aagtacggac gagtatcgaa ggtagagcgg cgagatcgcc aaggaggaat gcaatggcta
     1201 aggttaagaa aaacccagta attcaaggaa ttagcggcaa aatcggcaat ttggtgttta
     1261 agcaattgcc ggatggcagt acgatcgtga ctgccaagca ggtgcttcat aataagaagt
     1321 ggagtgaggg tcaggtggcg caccaggcgc ggatgcgaga agcggcggcg tacgggagag
     1381 aggcgcagca tcatccggtg tatgtggcgt tggcagagaa ctcgaccctg acggggtata
     1441 actgggcatt cggtgaccgg atgaagccgc cggtgatcca taaaatcgaa agagcagagg
     1501 gcttggtgcg ggtgcgggca agcgataacg tgggggtggt gcgggtggag gtgcaggtct
     1561 tggatgagaa gggagcggtt caggcgcggg gggaggctgt tcagacgagt aaagaggttt
     1621 gggagttcgc gacggagttg gcgggcaagg tggtggtgcg ggtgtgggat ttggcgcgga
     1681 atgtggtgga ggggagtttg gaatgatgaa ttaggagtta ggaattagga attaggaatg
     1741 aggggaaaaa ggaaggaagg aattggtaat tgggggtggt ggttaaacgt cgaggggaga
     1801 tttgacagcg ttggctccgc gctggagaac atgagtgtag atcatggtgg ttttgacatc
     1861 cttgtgaccg agtagctctt gtacggttct tatgtcgtaa ccggcttcga gaaggtgggt
     1921 ggcgaagcta tggcggaagg tgtgtggggt gacacgtttt tg
//
LOCUS       DAOREF010000654         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47535_length_1961_cov_3.041973, whole genome shotgun sequence.
ACCESSION   DAOREF010000654 DAOREF010000000
VERSION     DAOREF010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47535_length_1961_cov_3.041973"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PKI33_12650"
     CDS             complement(<1..1378)
                     /locus_tag="PKI33_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37910.1"
                     /translation="MKNPYRSYDLLWLSIALLPLIGLSFMLSVPAQDYWWYLRLGKDI
                     LEQGAIPLVDTMGYSRAGEPIFYQQWLSGIIFYLVYQAGNAPLTFLMRAILIGITYGS
                     IWHMMRSHVGARTATILLIILGMATSNNWMIRPQLFAYPLFLLCLFAIYEWQKGNNRL
                     LFLLPLSTFLWANLHGSFVLPFLLAGASLVFGAPNIELRSKGFLSALRELGGSKKHQA
                     MFITILAMLVASLLNPHGFGVWQYLVFILNNPSDYLFSVEWRPPTNEGWQMNIFFAWM
                     LALPILASFSKQKLTWLEWILFLGFGWLALSGVRYVIWFMFLLAIFTAKLISEWTNVY
                     IDKPIEKINPRINTSIAVMLLLLSLVFMPSVRDSWWEDAPDLYELTTTPVAAVEFLKT
                     REDLPDPLWNDYAFGSYLEFNLPSRPTWMDTRMYSFSLEQWQEYVRATNGEDWQEMFD
                     REGINLLLL"
     gene            complement(1421..1840)
                     /locus_tag="PKI33_12655"
     CDS             complement(1421..1840)
                     /locus_tag="PKI33_12655"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNM37911.1"
                     /translation="MDQEVHEFLLKHLQGIMTNDIMSYHETTSEDLTLYEWWVTPHRL
                     DGLPFHEFMMNSNAERGAVFGAEETGKTRFDLSNLLIQRYGDTAIASYTLLISTALGT
                     GVQVAAHNESRVMVKINGNWKVVHVHKSPAYSAPHVG"
BASE COUNT          538 a          452 c          505 g          466 t
ORIGIN      
        1 agagcaatag caagttgatg ccttcgcggt cgaacatttc ctgccagtct tcgccgttgg
       61 tcgcacgcac gtactcttgc cattgttcca gcgaaaagct gtacatgcgc gtgtccatcc
      121 aagtggggcg ggaggggaga ttgaattcga gatatgagcc gaaggcataa tcattccata
      181 gcggatcggg taagtcttca cgtgttttaa gaaattcaac tgcagcgact ggggttgttg
      241 tcagttcgta caggtctggt gcatcttccc accacgagtc gcggacgcta ggcatgaaga
      301 ccaatgaaag aagcaacagc atgactgcga tggaagtgtt gattcgcgga tttattttct
      361 caatgggttt gtcgatgtaa acatttgtcc attcgctgat gagtttggcg gtaaagatgg
      421 cgagcaggaa catgaaccag atcacataac gaacgcctga taacgccagc cagccaaagc
      481 cgaggaagag gatccactcg agccaggtca gtttttgttt ggagaacgaa gcgagaatgg
      541 gcagggcgag catccacgca aagaagatgt tcatctgcca gccttcattc gtgggtggac
      601 gccactcgac gctgaacaaa taatcggatg ggttgttgag gatgaagacg agatattgcc
      661 atacaccaaa tccatgcggg ttgagcaggc tggcaacgag catcgcgagg atggtgatga
      721 acatggcttg atgttttttt gaaccgccaa gctcgcgaag tgcgctaaga aaaccttttg
      781 atctgagttc aatattcggc gcaccaaaga caagagaggc tcctgccaag aggaacggca
      841 ggacgaacga gccgtgtaag tttgcccaaa gaaaagtgga cagcggtaga agaaagagca
      901 ggcgattgtt ccccttttgc cattcgtaaa ttgcaaagag acataataag aataaaggat
      961 acgcaaacaa ctgcggacgg atcatccaat tatttgaagt tgccatgcct agaatgatca
     1021 aaagaatggt ggctgtccgc gcgccgacat gagaacgcat catgtgccag atcgagccgt
     1081 aggtgatgcc gattagtatg gcacgcataa gaaacgtaag tggtgcgttg ccagcttgat
     1141 aaacaagata aaagataatg cccgaaagcc attgctgata aaagatcggc tcgcctgcac
     1201 ggctgtagcc cattgtatcc acgagcggaa tcgcgccttg ctctaaaata tctttgccca
     1261 agcgcagata ccaccaataa tcctgggcgg gtacggagag cataaaagac aacccaatta
     1321 atggaagcag agcaatgctg agccagagga ggtcgtaaga gcggtaggga tttttcataa
     1381 gattatgtag gggcgaggtc acctcgtctc tacgtgttaa ttatccaaca tgcggcgcgg
     1441 aatatgccgg tgacttatgc acatgcacaa ctttccaatt gccatttatc ttcaccatca
     1501 cgcggctttc gttatgtgcc gccacttgca cgccggttcc gagtgcagtg ctaataagca
     1561 atgtataact tgcgatagca gtatcgccat agcgttggat caataaattc gaaagatcaa
     1621 atcttgtctt gcccgtttct tctgcaccaa agaccgcgcc gcgctcggca tttgagttca
     1681 tcatgaattc atgaaaaggc aatccgtcta atctgtgcgg cgtgacccac cattcgtata
     1741 acgtcaggtc ttctgatgtc gtctcatgat atgacatgat gtcgttggtc atgatgcctt
     1801 gcaaatgttt caaaagaaat tcgtggactt cttgatccat gattaatcct ccaataaaac
     1861 tttgagatac gactcgacca tttgatcgag tccaaaagcg gactctgccc gcctccgtgc
     1921 cgctgctcgg aattgattct gtttctcaag cacctctccc g
//
LOCUS       DAOREF010000655         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47541_length_1961_cov_2.686779, whole genome shotgun sequence.
ACCESSION   DAOREF010000655 DAOREF010000000
VERSION     DAOREF010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47541_length_1961_cov_2.686779"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PKI33_12660"
     CDS             complement(<1..1296)
                     /locus_tag="PKI33_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM37912.1"
                     /translation="MESNNFFKKNLPTILWISAFISIYFVFSSLIAPYSSGISLDREI
                     VIGRLKEVGGFLFGILGVIAIIEIYFYQIFFRQPVNQVATFITRVIQVIVAILALTLV
                     IYFFNVTDLIRIQDGFFSKIINAPLGVQTNLKDLLTKSKIVSKETASIAASVILLLGF
                     LLVYAVIHFISSPRNSQVGLAYLLILPAFIGILFLIIYPFLFEIKLAFSNASLGTQAT
                     KNAEYGWIYGWENLKTLFTGTVAQDAKFMEVLWRTILWTAINVIFHVLGGMGLAILLN
                     RKLMFKGVYRTLLVVPWAIPTTIAAMALRNEFDSRYGLFNILLGDLNVWLTNISQYTH
                     SIIGVGNLFAWLAATIGPVSWKQDPVWAFVSVLIANIWLGIPFMSVIILGGLQSISQE
                     YYEAAEIDGATRWQQFRNITLPLLRPVLTPAIVLGVVWTF"
     gene            complement(1415..>1961)
                     /locus_tag="PKI33_12665"
     CDS             complement(1415..>1961)
                     /locus_tag="PKI33_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNM37913.1"
                     /translation="DGVVPAECDYACADTLMKEGKAAMIINGDWSLGDYTTALGDNLG
                     VAPVPTINGNPYTEMTAGKYFMVSKSVLDDAAKQAAVTTFITYMTSADVQKKWLDEFK
                     RLPSNSEVAKDPSIESDPILAGSMADLSVGRGQPAAPEMRCAWDAWRPNLEGVMAGTL
                     TPEDAAAAAQESADACVATLK"
BASE COUNT          598 a          417 c          428 g          518 t
ORIGIN      
        1 aaacgtccag accactccta atacgatagc aggtgttaat actggtctta ataatggcaa
       61 ggtaatattt ctgaactgtt gccagcgggt agcaccgtca atttctgccg cttcataata
      121 ttcttgagaa atgctttgca agccgcctag gataatgacc gacataaacg gaatacccag
      181 ccatatattt gcaattaaca cagaaacaaa cgcccataca ggatcttgtt tccaacttac
      241 aggaccaata gtggcagcta accacgcaaa aaggtttccg actccaatga tgctgtgtgt
      301 atattggctg atgttggtca gccagacatt aagatcgccc agcagaatat taaacaaacc
      361 atagcgacta tcaaactcat tgcgtaaagc catggctgca atggtcgtag ggattgccca
      421 tggtacgact agcaaagttc gataaacacc tttgaacatc aacttgcgat ttaaaagaat
      481 ggcaagtccc attccgccaa gaacatggaa aattacattg atcgctgtcc acagaatagt
      541 acgccaaagt acttccataa actttgcgtc ttgtgccaca gttccggtaa aaagggtctt
      601 gagattttcc catccgtata tccatccata ttctgcattc tttgtcgctt gtgttccgag
      661 cgaggcattt gaaaaagcaa gtttgatttc aaataagaat ggataaataa tgaggaaaag
      721 tatgccaata aatgcgggca aaattagtag ataagctaag cctacttgac tgttgcgcgg
      781 actactaatg aagtgtatga ctgcgtacac caataagaaa ccaagtaata aaattacact
      841 tgcagctata ctggcagttt ctttggagac tatttttgat tttgtaagaa ggtcttttaa
      901 gtttgtctgt acccccagtg gtgcgttgat aatcttgcta aaaaaaccgt cttgaatacg
      961 gatcaagtct gttacattga agaagtaaat aacaagcgtg agtgccaaga tggcaactat
     1021 aacttgtatt acccttgtaa taaacgttgc gacttgattt acgggttggc ggaaaaatat
     1081 ttgatagaaa tatatttcaa tgattgcaat gacacccaaa ataccaaaaa ggaacccacc
     1141 aacttccttg agccttccaa taacaatctc tcggtctaat gagattccac ttgaataagg
     1201 tgcaattaag gatgaaaaaa caaaataaat tgaaataaaa gctgatatcc acaaaatcgt
     1261 tggaaggttt tttttgaaaa aattattact ttccatattg cacctctgag ctgtcgttca
     1321 attattttat taagataatt atcccctgta tcatctttca ggatgtaaag gtggggagtt
     1381 ttcactcccc acctttatgg tactaaaaaa ctaattactt aagtgtggcg acacaggcgt
     1441 cagcagattc ctgtgcggca gcggcggcat cttcaggggt gagagtacca gccattacgc
     1501 cttcgaggtt ggggcgccaa gcatcccatg cgcaacgcat ttcaggagca gcaggttgac
     1561 cgcgaccaac agaaaggtca gccatggaac cagcaagaat tggatcactt tcaatgctcg
     1621 gatcctttgc aacttcagag ttggagggta agcgcttgaa ttcatccaac cacttcttct
     1681 ggacatcagc agaggtcatg taagtaatga aagtggtgac agctgcttgc ttcgcggcat
     1741 catcaagaac agacttggag accatgaagt acttgccagc ggtcatttcg gtgtaggggt
     1801 tgccgttgat ggtggggacg ggggcaacgc cgagattgtc gccaagggca gtggtgtaat
     1861 cgccaagcga ccagtcaccg ttgatgatca tggcagcttt gccttccttc atcagagtgt
     1921 cggcgcaagc gtaatcacat tccgcaggaa cgacgccgtc a
//
LOCUS       DAOREF010000656         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47614_length_1959_cov_5.115021, whole genome shotgun sequence.
ACCESSION   DAOREF010000656 DAOREF010000000
VERSION     DAOREF010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47614_length_1959_cov_5.115021"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKI33_12670"
     CDS             <1..412
                     /locus_tag="PKI33_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37914.1"
                     /translation="SGRKELTRTIMEFYRLGRDNVIDYTKDVYQQLNQHFLANKSNLF
                     KIIDGVNALHQKGQMSSADEGWNVVLGLYKSLGIKPEGIFGGEVILVPFNLETISRIP
                     KTVYAFNPMLFVCNSLEEMDEILDSFLKPIAMRK"
     gene            530..>1959
                     /locus_tag="PKI33_12675"
     CDS             530..>1959
                     /locus_tag="PKI33_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37915.1"
                     /translation="MAATYNFQWQNPNLLKSIYPMRLQKLRDFLVFYKEADLWAEYKN
                     KDISTLAADVKEYEDGMDLARATELKRYLSLKNYFMTKDVKGFFVKYKPIEEEALALI
                     QKNHDLFISSWPKDIRGERSFIQMRMESFKTQLGFLRDRIRYLQRQLEGMLPEHPNRP
                     KVEAEFKLKNESALPMVLDEMEKLRQFDDTYNKLEGPKLEWYKLAKADPNYKVTEEEF
                     LVNYDPKVPVTVKDIVRLKVEQYTKTLENKDQYQLLTDIRARFEKEPKRFPTWLQYMV
                     IHFSGMRYKSAHGSWANPKDLLVRLRVEEVKKAQAALSDADVTAKCAEKIAQYEGTDA
                     NKPALAKATDKAWKDKIALHMQGVKANGPKTKRAGLAALVEEEAKYEFLTMTTDQALA
                     KLEAMKDQIPAWAWKMIVMLTSLRVNYATDAGWETFTPEEDKARFADMTLYPILSKWA
                     TDNTGMWRDEHGRTQEIIVTRAVCNET"
BASE COUNT          563 a          500 c          487 g          409 t
ORIGIN      
        1 ctcgggacgc aaagagttga cccgcaccat catggagttt taccgcctcg ggcgtgacaa
       61 tgtgattgac tacaccaaag atgtgtatca gcaactgaat caacacttcc tcgccaataa
      121 gagcaacttg ttcaagatca tcgatggcgt gaacgcgctt caccaaaagg gacagatgtc
      181 ctctgcggat gaaggctgga acgtcgtttt gggcttgtac aagagtctgg gcatcaaacc
      241 cgaaggcatc tttggcggcg aggtcatcct cgtgccgttc aacttggaga ccatctcgcg
      301 catcccgaag acggtctacg ccttcaaccc aatgctcttc gtgtgcaatt cgctggaaga
      361 gatggatgag atattggatt cgtttttgaa gccaattgcg atgcgaaagt aaatttgagc
      421 aaactcccga agccttggct tcgggagttt gtgtttaatc tgcggttgaa aaatcgatta
      481 aattagctaa aatggaggga gtatttacat ctggcaccag gagagagaca tggcagcaac
      541 gtataacttt cagtggcaaa accccaattt acttaagagc atatatccca tgcgcctgca
      601 aaaattgcgg gactttctgg ttttctacaa agaagccgat ctatgggcag aatataagaa
      661 taaggatatt tccaccctcg ccgccgatgt gaaagaatac gaagacggca tggatttggc
      721 acgcgccaca gaactcaagc gctacctgag cttgaaaaac tatttcatga ccaaagatgt
      781 taaaggcttc ttcgtcaaat acaaacccat cgaagaagaa gcactggcgt tgattcagaa
      841 gaatcatgac ctgttcatct cctcttggcc caaagatatt cgcggtgaac gtagtttcat
      901 ccaaatgcgc atggagtcgt tcaagaccca gcttggcttt ttgcgtgacc gcattcgtta
      961 tttacagcgc caactcgaag ggatgttgcc cgagcacccc aaccgtccca aagtggaagc
     1021 ggaattcaag ctcaaaaacg aatcggcttt gcctatggtt ctggatgaaa tggaaaaact
     1081 ccgtcagttt gacgacacct ataacaagct tgaaggtccc aaactggaat ggtataagct
     1141 cgccaaagcg gacccgaact acaaagtgac cgaggaagaa ttcctcgtca actacgaccc
     1201 gaaggtgccg gtaaccgtca aagatattgt gcgcctcaaa gtggagcaat acaccaaaac
     1261 gcttgagaac aaagatcaat atcaactgct gacggacatc cgcgctcgct tcgagaagga
     1321 acccaaacgc ttcccaactt ggttgcaata catggtgatt catttctcgg gcatgcgcta
     1381 taagtcggcg catgggtcgt gggcgaaccc aaaagatttg cttgtgcgct tgcgtgtgga
     1441 agaagtgaag aaagcccaag ccgccctcag cgatgcggat gtgaccgcca aatgcgccga
     1501 gaagattgcc caatacgaag ggacagatgc caacaagccc gccctcgcca aagcaaccga
     1561 taaggcgtgg aaagacaaga tcgccctgca catgcaaggc gtgaaagcca acggacccaa
     1621 gaccaagcgc gccggtcttg ccgctctggt ggaagaagaa gccaaatacg agttcctgac
     1681 catgacgacc gaccaagccc tggcgaagtt ggaggcgatg aaagatcaaa tcccagcttg
     1741 ggcgtggaag atgatcgtca tgctcacatc cttgcgcgtt aattacgcca ccgatgccgg
     1801 ttgggaaact tttacgcccg aagaagacaa agcccgtttc gccgatatga ccctctaccc
     1861 catcctcagc aaatgggcaa ccgataacac cggcatgtgg cgcgacgaac acggacgcac
     1921 ccaagagatc atcgtcacac gcgcagtctg caatgagac
//
LOCUS       DAOREF010000657         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47671_length_1958_cov_3.460851, whole genome shotgun sequence.
ACCESSION   DAOREF010000657 DAOREF010000000
VERSION     DAOREF010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47671_length_1958_cov_3.460851"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKI33_12680"
     CDS             <1..872
                     /locus_tag="PKI33_12680"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF025913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BglI family type II restriction endonuclease"
                     /protein_id="HNM37916.1"
                     /translation="WCPPNRKISMPPQTNRTQLEKFLTNHLVSIITANIIEIKQDYNE
                     ASYLYPFWENYPPEDRGRQPIKDQYPWIEVGEHAIGSKLPRLLDSAFRVRDTGLPTGS
                     DQRFVLTDDAIATATGGFTNSVWFFVDIKSVGPRDDQHHTVMSHNQVSGDGIWINPVD
                     GVKNTILQATGARTSHDFHASLPPVFVLSDGTIAPLVMIALKPVYRMLQTNVVGARND
                     GQPLERIDIACIPNGLLLTQQPNYLGTYNGLLFPGKDDKSKDPRKLRARVSFEILKKI
                     APWRVQTIQVPFP"
     gene            complement(869..1813)
                     /locus_tag="PKI33_12685"
     CDS             complement(869..1813)
                     /locus_tag="PKI33_12685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNM37917.1"
                     /translation="MNFLEVNKIHQGRSEILMKQIEPESVALSFWSPPYFVGKEYEKD
                     ETFDSWQAMLRQVIHHHSKALKPGGFLIINIGDIRCFKDESIPRFQAMNISMQKSKIT
                     REMVLEAKAQFPEFSRYQLAEHLGCSEQTIDRRLNGNNIRGGKYEIQTRVNLVGGPLQ
                     TYGQECGLYLYDHRIWVKDPAWANCKWTSNSLKSVDEYEDLYVFWKPGQQIIDRKKLE
                     PQEWKDWGSRAIWFVKSVRANDDHEAKFPLELASRVVRLYTDKDDVVLDPFMGSGTTG
                     IACVRHGRKFIGIEKEAKYVALAQENIKKELSQLKLNI"
BASE COUNT          549 a          433 c          388 g          588 t
ORIGIN      
        1 gttggtgtcc acccaaccgc aaaatttcaa tgcctccaca aaccaatcga actcagcttg
       61 agaagttttt gacaaaccac cttgtttcga taattactgc aaatattatt gaaatcaaac
      121 aagattacaa tgaagcatct tatctctacc ctttttggga gaactatcca cctgaagata
      181 gggggcgcca gccaattaaa gaccagtatc cttggattga ggttggggag cacgcaatcg
      241 gttcaaaatt gccaagatta ttagattcgg cattccgagt tagagacaca ggattaccaa
      301 cgggatcaga tcagcgattt gtactcactg atgatgcgat tgcaacagca acaggtggat
      361 ttacgaattc agtatggttt ttcgtggaca tcaaaagtgt aggcccaaga gatgatcaac
      421 atcacacagt catgagtcac aaccaagtct caggcgatgg tatttggata aatcccgtgg
      481 atggggttaa aaataccata ttacaagcaa ctggggcgcg gacgtcacat gattttcacg
      541 caagcctccc gccggttttc gttctttcag atggcacgat tgccccgctt gtaatgattg
      601 ctttaaaacc tgtgtatcga atgttgcaaa caaatgttgt aggcgctaga aatgatgggc
      661 agccattgga aagaattgac attgcctgca ttccgaatgg acttttgtta acgcagcagc
      721 cgaattatct tggcacgtat aacggtttat tatttccagg aaaagatgat aaatccaaag
      781 acccaagaaa actacgagct cgtgtcagct ttgaaatctt aaaaaaaatt gcgccatgga
      841 gagtgcagac cattcaagtg ccgtttcctt aaatatttag cttaagctgg ctcagctcct
      901 tttttatatt ttcttgtgcc agcgctacat atttagcctc tttttcaatc ccaatgaatt
      961 tccttccgtg acgaacacaa gctatgcctg ttgtgccaga tcccataaaa ggatccagca
     1021 caacatcatc tttatctgtg tatagcctta caactcttga cgcaagctcc agtggaaatt
     1081 tagcttcatg gtcatcgttt gcgcgcacac ttttaacaaa ccatatagct ctcgatcccc
     1141 aatccttcca ttcttgtggt tctaattttt ttctatctat gatttgctgc cccggtttcc
     1201 aaaaaacgta aaggtcttca tattcatcaa ccgatttaag cgagtttgaa gtccacttgc
     1261 aatttgccca tgctgggtct ttaacccaaa ttctgtggtc atacaaataa agaccacatt
     1321 cttgcccgta tgtttggagt ggcccaccca caagatttac tcttgtctgt atttcatatt
     1381 tcccaccccg aatattattt ccgtttagcc gcctatcaat tgtttgctca ctacaaccaa
     1441 gatgctctgc tagttgatac cggctaaatt caggaaattg tgcttttgct tcaagcacca
     1501 tttctctggt gatttttgat ttttgcatag agatgttcat tgcctggaag cgtggaatgc
     1561 tctcgtcttt gaaacaacga atatcaccaa tatttattat taggaacccg cctggtttga
     1621 gtgcctttga atgatggtgt atgacttggc gaagcatagc ctgccaagaa tcaaaggttt
     1681 catccttttc gtattcctta ccaacgaagt aaggcggaga ccaaaagcta agagctacag
     1741 attctggttc aatctgtttc attagtattt ctgatcggcc ttgatggatt ttattaactt
     1801 caagaaagtt cattttactt ccagtacagt tttgtatgtg actcatagta tacaaaacac
     1861 tgcgatttgg caaccgctat gaaggggcgg gcttatgcgt ataactctac gttatctgac
     1921 gtagaaacgt ggattccata gcgcggaaac aaccagag
//
LOCUS       DAOREF010000658         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47672_length_1958_cov_3.434577, whole genome shotgun sequence.
ACCESSION   DAOREF010000658 DAOREF010000000
VERSION     DAOREF010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47672_length_1958_cov_3.434577"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PKI33_12690"
     CDS             <1..1091
                     /locus_tag="PKI33_12690"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNM37918.1"
                     /translation="TLVPMYFLIGIWGGPRRVYAAVKFFLYTMAGSILMLLAIIYLGI
                     QAQNTFNVPDLLKKLPELYASGAIPAGVQMMLFIAFTAAFAIKVPMWPLHSWLPDAHV
                     EAPTAGSVILAGVLLKMGTYGFLRFNIQLFPEATIKAAPWIALLATIGIIYGAAVSYA
                     QQDVKKLVAYSSVSHLGFVMLGLFALNPEGVAGAILQMVNHGLSTGALFLLIGMIYEQ
                     THTREIKVYGGLWKITPIFGTIMLIASLSSMGLPGLNGFVGEFTILLGAFGSKAIGSP
                     WFAGLSALGVIMAAVYILYMFQKVFLGPAGEITHHHELKDLNWREILTVAPLVIFMFW
                     IGLYPKPFFDILAPAVEKLLLPITALLH"
     gene            1205..>1958
                     /locus_tag="PKI33_12695"
     CDS             1205..>1958
                     /locus_tag="PKI33_12695"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNM37919.1"
                     /translation="MTQPTYTDFYTLIPYLILTVWACVLLIVDLFIPKGRKGITAFLA
                     ALGIAISLGYSITQTGIESTGFSSMVSLDGFSTFANILLLGSGLFAIALAYGYTKRMG
                     IERGEYYTLLLFSLTGMMLMAQANDLIMVFIALELLSIPLYVLSAISRKLTSEESGVK
                     YFLLGAFASGFVVYGTALIFGATGSTNLNVILVQAASGVTSGLLLTIGAALLLVGFGF
                     KVAAVPFHMWTPDVYQGAPTSVTAFMAAGAKIA"
BASE COUNT          381 a          554 c          453 g          570 t
ORIGIN      
        1 tcacgctcgt gccgatgtac ttcctcatcg gcatctgggg cggaccacgc cgcgtatatg
       61 ctgctgtcaa gttcttcctt tacacgatgg ctggttccat tctcatgctt ttggcgatta
      121 tttacctcgg cattcaagcg cagaatactt tcaacgtacc agatttgttg aagaaacttc
      181 cagagttata tgcttctggc gctatccctg ctggcgtgca aatgatgctt tttattgcat
      241 tcaccgctgc cttcgccatc aaggtcccga tgtggcctct ccattcgtgg ttgcctgatg
      301 cccacgtgga agctcccacc gcgggttccg ttatccttgc aggtgtcttg ctaaagatgg
      361 gtacctacgg cttcttgcgc ttcaacatac aactcttccc tgaagctacc atcaaagccg
      421 ctccgtggat cgcacttctc gcgactattg gtattatcta cggcgctgcg gtttcttatg
      481 cccaacaaga tgtgaagaag ctggtcgctt attcctcagt gagccacctc ggttttgtga
      541 tgcttggttt gtttgctctc aatcctgaag gcgtagctgg tgcaattttg caaatggtca
      601 atcacggtct cagcaccggc gcgttgttcc ttctcatcgg tatgatctac gaacagactc
      661 acacccgcga gatcaaagtc tacggcggtt tgtggaagat caccccgatt ttcggcacca
      721 tcatgctcat cgcctcactt tcttcgatgg gcttgccggg tctcaacggc tttgtgggtg
      781 agttcaccat cttgctcggt gccttcggtt caaaagccat cggtagccct tggtttgcgg
      841 gtctttccgc tctcggcgtc atcatggcgg cagtctacat tctttacatg ttccaaaagg
      901 tctttttggg acctgccggt gagatcacgc atcaccatga gctcaaagac ttgaattggc
      961 gcgagatcct caccgtcgct ccgctcgtta ttttcatgtt ctggatcggt ttgtacccca
     1021 agcctttctt cgatatcctc gcccctgcgg ttgagaagtt gctgttaccg ataacggcct
     1081 tgttacatta ataatatttt tgtcactgcg cgggtgaaac ccgaaacaga ctcagacgta
     1141 accatgagat tttgtttctt cagaaagaat acctgccttg caatgacaaa gacatggagc
     1201 gttcatgacg caaccaacct acaccgactt ttacacactc atcccttatc tcatcctgac
     1261 cgtttgggca tgtgtgttgc tgattgttga cctattcatt ccaaaaggtc gcaaaggcat
     1321 cactgcattt cttgctgcat tgggcatcgc catctcgctt gggtattcaa ttacgcaaac
     1381 aggtattgaa agcaccggct tcagcagcat ggtttcgctt gacggcttct ccactttcgc
     1441 caatatcctt ttgctcggaa gtggactctt cgccatcgcc cttgcctacg gctataccaa
     1501 acgcatgggc atcgaacgcg gcgagtacta tactctgctg ttattcagcc tcaccggcat
     1561 gatgctcatg gcacaagcga acgacctcat catggtcttc attgcgctcg aattgctttc
     1621 catcccgctc tatgtcctct ctgcgatctc ccgcaagctg acctctgaag aatctggcgt
     1681 gaaatacttc ttgctcggcg ccttcgctag cggatttgtg gtctatggaa ccgctctgat
     1741 cttcggtgcg acgggctcta ccaacctcaa tgtgatcttg gttcaagccg cctctggcgt
     1801 gacctcgggc ttgctcctca ccattggggc tgcgcttttg ctggtaggct ttggcttcaa
     1861 agtagccgca gttcccttcc acatgtggac ccccgatgtc taccaaggtg caccaacttc
     1921 tgtcacagct ttcatggcag ctggtgcgaa gattgctg
//
LOCUS       DAOREF010000659         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47711_length_1957_cov_4.267087, whole genome shotgun sequence.
ACCESSION   DAOREF010000659 DAOREF010000000
VERSION     DAOREF010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47711_length_1957_cov_4.267087"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PKI33_12700"
     CDS             complement(<1..459)
                     /locus_tag="PKI33_12700"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNM37920.1"
                     /translation="MSSQPIPVIEARGTYREVGKQIGEQCKPQIARMMSQLHESLPPG
                     KTWEMLVNESRIYQTHSRAVYPQYMDELEGIAEGAGVPFDELFISMLEELWETPPYGK
                     GCTDMAARGRATLDGSTLVAHTNDLSAKTEEDLVILKIQAGDEPEFIGVSA"
     gene            complement(534..1412)
                     /locus_tag="PKI33_12705"
     CDS             complement(534..1412)
                     /locus_tag="PKI33_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM37921.1"
                     /translation="MNSKSFTRLSVLVVLAMLAALIPLQVHAGGVCGGAYTIEKGDTV
                     DKLASMCGTTASAIYATNPGLKEPLTVGQVITIPGANYGVTTTPDAAATPTPTAVVGN
                     ATAMPAAVVNYYTYNYYNYYYANPTVTTSTTYIVQAGDTFATIAARYGLSVSQLWAAN
                     PQLTDINVIYIGQLLYIPANTYYPTAVPSAVATQELKKHVDNSQVPSNAAYATIWLIN
                     KSEADVYISLRTSRADGTNAINEFPVGRQMDVEIPTGWVDYTAYVGGRKFTGGFMVRE
                     GSQHTITFNRSKVVVD"
     gene            1590..>1957
                     /locus_tag="PKI33_12710"
     CDS             1590..>1957
                     /locus_tag="PKI33_12710"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNM37922.1"
                     /translation="MNTTYQQILDEIYQETLPLLGQGKVASYIPALAEVNPKQYGIAL
                     QTLSGETYQVGDANTLFSIQSISKVFTLAMVFGKVGEALWTRVGREPSGNAFNSLIQL
                     ETENGIPRNPFINAGALVVT"
BASE COUNT          486 a          452 c          498 g          521 t
ORIGIN      
        1 cgctgaaacg ccgataaatt ccggctcatc gcccgcttga attttgagaa tgacgaggtc
       61 ttcttctgtc ttggcggaga ggtcattggt atgtgctacg agagttgagc cgtcgagcgt
      121 ggcgcgtccg cgggcagcca tgtcggtgca gcctttgccg tagggcggag tctcccataa
      181 ttcttccagc atcgaaatga aaagctcgtc gaatggcaca cctgcacctt cagcaatacc
      241 ttcaagctcg tccatgtatt gcgggtagac ggcacggctg tgagtttggt agatacggct
      301 ttcgttcacg agcatctccc aggttttgcc gggtggcaaa ctctcatgca gttgactcat
      361 cattctagcg atttgtggtt tgcattgttc cccaatttgt ttgccgactt cacgatatgt
      421 tccgcgtgct tcaataacgg gaataggctg gctggacata cattctcctt gaatcaaaaa
      481 tgacccgcta agtgtatcag aacacgaagc gggtcatttt atggtcttaa cgattaatca
      541 acgacgactt tactgcgatt gaaagtaatg gtgtgttgcg aaccttctcg aaccatgaaa
      601 ccacctgtga actttctgcc accaacatag gcagtatagt ccacccaacc ggtcgggatt
      661 tcaacatcca tttgccttcc aacagggaat tcattgatgg catttgttcc atctgcgcgg
      721 ctggtgcgca acgaaatata cacatccgct tcagacttgt tgatgagcca aatggtggca
      781 tacgcagcat tgcttggaac ttgggaatta tccacatgct ttttcaactc ttgcgtagct
      841 actgctgaag gaaccgcggt gggatagtag gtatttgccg gaatataaag taactgaccg
      901 atatagatta cgttgatatc ggtcaactgc gggttggcag cccaaagctg agaaacgctg
      961 agcccatacc gggcagcgat ggtcgcgaat gtatcaccag cttgaacaat ataagtagta
     1021 ctagttgtta ccgtggggtt ggcgtaataa taattgtagt agttataggt atagtaattg
     1081 actacagccg caggcatggc ggttgcattt ccaaccaccg ctgtgggggt gggcgttgca
     1141 gccgcatccg gtgtagtggt cacaccatag tttgctcccg gaatggtgat cacttgccca
     1201 actgtgagcg gctctttcaa ccccgggttg gtggcataga tcgcggatgc ggttgtcccg
     1261 cacatggacg caagcttatc gacggtatcg cccttttcaa tggtataggc tccaccgcat
     1321 acgcctcctg cgtggacctg aagcggaatc agcgctgcca gcattgccag caccaccaaa
     1381 acgcttaacc gagtaaatga ttttgagttc aaaactgcct ccttgcacgg tctactttaa
     1441 ttcgtataac cctatgataa gccaaaaaga gtcgaaaggc aactggtttt gtaagaaaaa
     1501 ttgcttacaa atacataaca tggctccatt tttgcgaaaa tagggctttt gagcgaggct
     1561 tgataaaatt atccgtacgg agaactgaat tgaatacaac ctatcaacaa attttagatg
     1621 agatctatca ggagaccctg cctttgcttg gacaaggcaa agttgccagt tatatccctg
     1681 ctttggcaga agtgaaccca aaacaatatg ggatcgcgct ccaaaccttg agcggcgaga
     1741 cgtatcaagt aggggatgcc aatacgcttt tctctattca gagtatttcg aaggttttta
     1801 cgttagcgat ggtgtttggg aaagtgggcg aggcgttgtg gactcgggtt ggacgcgaac
     1861 cttcgggtaa tgcctttaat tcgttgatcc agcttgagac agagaatggg attccgcgta
     1921 atccttttat caatgccggt gctttggtag tgaccga
//
LOCUS       DAOREF010000660         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47786_length_1956_cov_2.070489, whole genome shotgun sequence.
ACCESSION   DAOREF010000660 DAOREF010000000
VERSION     DAOREF010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47786_length_1956_cov_2.070489"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PKI33_12715"
     CDS             complement(<1..1619)
                     /locus_tag="PKI33_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNM37923.1"
                     /translation="MKSILGVSLGHDTSFAHIVNGKVVAVMEAERYFRQKRYKLHCLT
                     MEPGKHISGYQYVSLDDIELFLGMVAKEWGTSFDALAVQNQGRVEEFKNFQQILEKLG
                     FKFGSVHHVDHHLSHAALAFFSSSFKDALVLSYDGEGNDGQTIIFGAKGNTIEYLHND
                     PVRYGQSYNNMGYIVGIKPEVEGTTSGKVMGLTAYGEPVQDWLPYARDYIKKYVKATP
                     RQIEGLNNYGKAHRINDIGVRDIPDLKKFLVPEDEKNTGLKAAVKKATGIKNTVLRLN
                     GPEDKNAQNFAATVQAAWTESVIDLLKPYFGKYENLCVVGGCALNGVTNYEIQQLGVF
                     KATHFVPNPSDCGLSAGAALYVYWNSGGNGTFDGYGEYFSPYLGSEVFDKNDLPALRS
                     QYPNLDLDSELVATILAKLVHLDYMVGVIRGRYEVGPRALGNRSILCNPLNKDMKDIV
                     NKKVKHREWYRPFAPIAISERAADYFTNTSDIPYMSVICYTKPEYQEVLPSITHVDGS
                     SRLQTVGQDAHPFMHRTLKEFEKLTGMPIML"
     gene            complement(1661..>1956)
                     /locus_tag="PKI33_12720"
     CDS             complement(1661..>1956)
                     /locus_tag="PKI33_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37924.1"
                     /translation="NVPPDVVIRFGETIPPRDWKSFTVLVRDVTKDLITQTPQSPFEE
                     LHHQNQELFDALENYLGDRTLDRDARRIYAEAECGELDGGAGRRLVNMIKNRI"
BASE COUNT          531 a          460 c          419 g          546 t
ORIGIN      
        1 ttaagcatga tgggcatacc tgtaagtttt tcgaactcct tcaatgttct atgcatgaaa
       61 ggatgggcat cctgcccaac agtttgaagg cgggagcttc catccacatg ggtaattgaa
      121 ggcaatacct cctgatattc gggtttggta taacagatca cagacatgta ggggatgtct
      181 gaagtgttgg taaagtaatc tgcagcacgc tctgagatag caatcggtgc aaaggggcga
      241 taccattcgc ggtgtttaac ctttttgttg acgatgtcct tcatatcctt gttaagtggg
      301 ttacacagta tggaacgatt gcccaaggca cgcggtccaa cctcatatcg tccgcggata
      361 acccccacca tgtaatctaa atgaaccagc tttgcaagaa ttgttgcaac cagttcggaa
      421 tcaagatcta aatttggata ttgggaacgc agagcaggta aatcgttctt gtcaaagact
      481 tctgaaccca agtaagggga gaaatattcg ccatacccgt caaatgttcc gttcccacca
      541 gagttccaat aaacgtaaag agcagctcct gcagacaaac cgcagtcgga tgggtttggg
      601 acaaaatgag tcgccttgaa aacccctagt tgttggattt cgtaattggt gacgccattc
      661 aaggcacatc cacccactac acaaaggttt tcatactttc caaaataggg tttgagcaag
      721 tcaatcacag actcagtcca tgctgcttga acggtggcgg caaaattttg tgcattttta
      781 tcttccggtc catttagacg cagaaccgta ttcttgatac ctgtcgcttt tttgaccgcc
      841 gccttcaaac cggtattctt ttcatcttct ggaacaagaa attttttgag gtcgggaata
      901 tcacggacgc caatatcatt gatgcgatgc gccttgccgt agttattgag tccttcgatc
      961 tgtcgggggg ttgccttgac atacttcttg atatagtctc gggcataggg cagccaatcc
     1021 tgcactggtt caccataggc agtgagaccc atcaccttac cagatgtagt tccttccacc
     1081 tcaggtttaa ttccgacaat gtaccccata ttgttataac tctgcccata tcgaacagga
     1141 tcattgtgca agtattcgat cgtattacct tttgcgccaa aaataatggt ttgcccgtca
     1201 ttgccttcac catcatagct aaggacaaga gcatctttga atgaagatga gaaaaaagcc
     1261 agtgcagcat gactcaagtg atgatctaca tgatgaacag atccaaattt aaatcccagt
     1321 ttttcaagaa tttgctgaaa gttcttgaac tcttccacac gtccctgatt ttgaacggca
     1381 agggcatcaa acgatgtacc ccattcttta gccaccatac caaggaatag ttcaatatca
     1441 tccagagaaa catattgata tccactgata tgtttaccag gttccattgt caggcaatgt
     1501 aatttatagc gcttctgacg aaaataacgt tcagcctcca taacggcaac aactttgcca
     1561 ttcacaatgt gtgcaaagct cgtatcgtgt cctaaactta cacctaaaat acttttcatt
     1621 ttatcctcca ggatatgatt gagttttgat gataaccata ctagattctg tttttaatca
     1681 tattgactaa acgcctgcct gccccaccgt ccaactctcc acattcagcc tcagcataaa
     1741 ttcgacgtgc atcacggtca agagtacggt cgccaagata attctcaagc gcatcaaaga
     1801 gttcttgatt ctggtggtga agttcctcga agggactctg cggggtctgc gttatcaggt
     1861 cttttgtcac atcccggacg agcacggtga aacttttcca gtcgcgcggg gggattgttt
     1921 cgccaaagcg gatcacgaca tcgggcggga cgttgg
//
LOCUS       DAOREF010000661         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_47861_length_1954_cov_3.705635, whole genome shotgun sequence.
ACCESSION   DAOREF010000661 DAOREF010000000
VERSION     DAOREF010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47861_length_1954_cov_3.705635"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..957
                     /locus_tag="PKI33_12725"
     CDS             58..957
                     /locus_tag="PKI33_12725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM37925.1"
                     /translation="MNPYRRSPGVIAATVFVYFFLYAPIVMLILYSFNSSRANVKFEG
                     FVTSFSDRVKMDGSTVVSSPCGPFHWYCDLAKNDDVIEAAGNTLSIAFTATIISTIIG
                     TMAALALQRYDFKIKPFSQLALYIPIVIPEIVMGIGILTLFSQAFGWLNNALNLTGDA
                     RLALGIGTVTVSHIAFMVPFVTLVVQARLQGFDKSYEEAAMDLGANEWITFRRVTFPM
                     ILPGVLSGALLAFTLSLDDFVITFFTNGPGSTTLPIYVYGLLRRIITPQVNALSTVWI
                     LTVFIAVVVLQWIQSREQKPSHS"
     gene            1015..>1954
                     /locus_tag="PKI33_12730"
     CDS             1015..>1954
                     /locus_tag="PKI33_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNM37926.1"
                     /translation="MLRKSLFNILAVLAILSFALTACGGGGGDAEAPAAGDGPQVTSS
                     GFECPEPNPRLDVTSTELNIFVWTEYFPQDMLDCFELVYGITLNRDEYSSNEEMYAKV
                     AAGGTAYDLAQPTDYIVPLMIRQELVQELDHSKLPLLSNFDPNWMDQPFDPGNKYSLP
                     YLAGTDAIVVNTATVENVPTSWADLWNPEYAGRMVFLDDSRAVIGLTLVTLGYDPNTT
                     DPAQLEEAKNKLAELVPNIKLFDSDSPKTALIAGDVDLGMTWTGEAFIANQENPDIQY
                     VYPEEGPVLWQDNWVMLKDASHADAAYAWISYIMQGD"
BASE COUNT          443 a          490 c          475 g          546 t
ORIGIN      
        1 agatcattgc cgcatgatcc catacgctac aaaccttttt attaaggagg aaatgcgatg
       61 aatccgtatc gccgttcccc tggggtaatt gccgccacgg tctttgtgta tttctttctg
      121 tacgcaccga ttgtgatgct gatcttatat tcctttaact cttcacgtgc gaatgtgaag
      181 tttgaagggt ttgtcacatc cttcagtgac cgggtgaaga tggatggcag tacggtcgtg
      241 tctagcccat gtggtccctt tcactggtat tgtgaccttg caaaaaatga tgatgtgatt
      301 gaagccgcag gcaatacgtt aagcatcgcg tttactgcta cgattatttc taccatcatt
      361 ggtacgatgg cggctcttgc gctgcaacgt tacgatttca aaattaaacc tttctcgcaa
      421 ctggctttat atattcctat cgtgatccct gagatcgtta tggggattgg tattctgaca
      481 ctgtttagtc aggcttttgg atggctaaac aatgctctca atttgacagg tgacgctcgc
      541 ctggcattgg gcattggcac ggtgactgtt agtcatatcg cgttcatggt gccgtttgta
      601 acgctcgttg tgcaagctcg cttgcaaggc tttgacaagt cttacgaaga agccgccatg
      661 gatttgggcg ctaacgaatg gatcacattc cggcgtgtaa ccttcccaat gattttaccg
      721 ggtgtgctct cgggtgcttt gcttgccttc accctttctt tggatgactt tgtaatcacc
      781 ttcttcacga atggacctgg ctctacgacc ttgccgatct acgtatatgg tctgttacgg
      841 cgcattatta cgccgcaagt gaatgcgctt tcaacagttt ggattttgac cgtttttatt
      901 gcggttgtag tcttgcaatg gattcaaagc cgcgagcaga aaccctctca ttcctaatct
      961 gggtatatcg tgcgtgagac ccgcacgttg ctaactatat aaaggagaag aaacatgtta
     1021 cgaaaatcgc tattcaatat tcttgccgtt ctggcgatcc tcagctttgc gctgaccgcc
     1081 tgtggtggag gcggtggaga tgcggaagcg cccgccgccg gggatggtcc tcaagtgacc
     1141 tcttctgggt ttgaatgccc ggaaccaaat ccgcgtttgg acgttacctc caccgagttg
     1201 aatatcttcg tgtggacgga gtacttccca caagatatgt tggactgttt tgaactggta
     1261 tatggcatca cattgaaccg cgacgaatac tcctccaatg aggaaatgta cgcgaaggtt
     1321 gcagctggtg gtaccgccta cgatctggct cagccaaccg attacatcgt gccgctcatg
     1381 atccgtcagg aattggttca ggaactggac cactccaaac ttccacttct tagcaacttt
     1441 gacccgaact ggatggatca gcccttcgac cccggtaaca agtactcact gccatatctt
     1501 gctggcacag acgccattgt tgtgaacact gcaactgtcg aaaatgtacc gacctcctgg
     1561 gcagacctgt ggaatccaga gtatgccgga cgcatggtct tcctggatga ctctcgtgct
     1621 gtgatcggtc tcaccttggt aacccttggc tatgacccca acacaaccga ccccgctcaa
     1681 ttggaagaag ccaagaataa gttggcagaa cttgttccga atatcaagct cttcgatagc
     1741 gatagcccca aaaccgccct catcgctggc gatgttgacc tcggcatgac ctggaccggt
     1801 gaagccttta ttgccaacca ggaaaaccct gatattcaat acgtctaccc tgaagaaggt
     1861 cctgttcttt ggcaggataa ctgggtcatg ttgaaggacg cttctcatgc cgatgccgcc
     1921 tatgcatgga tcagctacat catgcaaggc gatg
//
LOCUS       DAOREF010000662         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48110_length_1949_cov_3.894403, whole genome shotgun sequence.
ACCESSION   DAOREF010000662 DAOREF010000000
VERSION     DAOREF010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48110_length_1949_cov_3.894403"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PKI33_12735"
     CDS             complement(<1..1324)
                     /locus_tag="PKI33_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM37927.1"
                     /translation="MKNLLNDLKSAIYPIADLRTEESRREHTARAVFIIIGFCLALST
                     LAVTVLDLIAEKPQYWPIFLTAGLLFLHFLGWLLVQNGRWKEARYLPPALFLIAGSVL
                     TANKELGTIAILQFTLSIMLTAILFETSAQWVSIFISAASYLGIAHFIGEQGSYTERQ
                     LQYINVVVFVAIGVLQQITSGVISLTIKRLTLETETRKKTEESALQKEHILSVIAQSA
                     QLLLDAQNWEDKIDEMLALLGSVSGASHAYLFKNHTDENGRLLTSLKYEWKNPEQDHP
                     SNPDSFQNIPLLEEEMVTWYNLLITGKPFYESTDFFSPEWTEQNKDREGIKTLLDVPV
                     FVDGEWWGVIGFDDYQRVTPWSQAEVDALQVAASLLGSAIKRQRSTDELTASEDKFQK
                     TFQQTMVPMVIGRISDRVIVDANDAFAKLTGYSRAEVVNRWASELNLWQ"
     gene            complement(1346..>1949)
                     /locus_tag="PKI33_12740"
     CDS             complement(1346..>1949)
                     /locus_tag="PKI33_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37928.1"
                     /translation="NACTLDEVQNSQVAYKKFRGTKPLGYRAPIGRIDKNGLGHLLDF
                     GFEYDASVYPSVRPGEFGYFNLHMPNMPFRVVREDGKSLVEFPFTSIETIRIVFALSY
                     AKLLGWFGYSSLLKLFGLPEITLLLSHPHDFYFGSIPNKTVGGLEGFAMSRNTGKEFD
                     IFEKMLQTLQQQGYEFVYLSEVHQQVKGSELQTFAWEEWK"
BASE COUNT          569 a          422 c          391 g          567 t
ORIGIN      
        1 tttgccaaag attaagctca gacgcccagc gattaacaac ttctgcgcgt gagtaccctg
       61 ttagctttgc aaaagcatca ttagcatcta cgatgacccg gtctgatatc ctgccaatga
      121 ccattggaac catggtctgt tgaaaggtct tttggaattt atcttcagaa gcggttaatt
      181 catctgtact gcgttgacgt ttaatcgccg atcccaataa gcttgctgca acttgtaatg
      241 catccacttc ggcttgcgac catggtgtga cacgttgata gtcatcaaac ccaataacac
      301 cccaccattc accgtctaca aaaaccggaa catccagtaa ggtttttatg ccttctctat
      361 ctttattttg ctcagtccat tctggcgaga aaaagtcagt agattcgtaa aaaggctttc
      421 cggtaataag gaggttatac caagtgacca tttcctcttc aagcaatgga atattctgaa
      481 atgagtcagg gttggatgga tgatcctgct cggggttttt ccactcatac tttaggctgg
      541 ttaatagccg cccgttttca tccgtgtgat ttttgaataa atacgcatga ctggcgccag
      601 aaactgagcc taataatgcc aacatttcat caattttatc ttcccagttt tgggcatcta
      661 gtaaaagttg ggcagattgt gcaatgacag aaagtatatg ttctttttga agtgcagatt
      721 cttccgtctt tttcctagtt tctgtttcca aggtcaaacg tttgatagtt aggctgataa
      781 cccctgatgt aatttgttgt aataccccaa ttgctacgaa aacaaccaca ttgatgtatt
      841 gcaattgtct ttctgtatat gaaccttgtt ccccgataaa atgagctatt cccaaataag
      901 acgctgcgct tataaaaata gatacccatt gtgctgatgt ttcaaataga atagcggtca
      961 gcataatact gagggtaaat tgtaggattg caatggtacc tagttcttta tttgcggtaa
     1021 gaactgaacc agcaatcagg aataaggcag gcggaagata tctcgcttct ttccaacgcc
     1081 cattttgtac cagaagccat ccaagaaagt ggaggaataa caaccccgca gttagaaata
     1141 tgggccagta ttgaggtttt tcggcaatga gatcaagaac tgtaactgca agagttgata
     1201 aagcaaggca gaatccaatg ataataaaaa ctgcccgagc agtgtgctcg cggcgtgatt
     1261 cttccgtccg tagatcggcg attggataaa ttgctgattt gaggtcgttc agtagatttt
     1321 tcatcttcga acgttcagga ttcgactact tccactcctc ccatgcaaaa gtttgtaact
     1381 cagagccttt tacctgttga tgcacttcgc tcaagtagac aaactcataa ccttgttgtt
     1441 gaagagtttg cagcattttt tcaaaaatat caaattcttt ccctgtatta cgcgacattg
     1501 caaaaccttc caaccctcca actgttttat tgggaatgct cccaaaataa aaatcatgcg
     1561 gatgagaaag taggagtgta atttcaggca acccaaaaag ttttagcaaa ctagaataac
     1621 caaaccagcc taaaagcttt gcgtagctaa gcgcaaatac aatgcggata gtttcaatcg
     1681 atgtaaatgg gaattccaca agcgatttgc catcttcacg taccactcga aacggcatat
     1741 ttggcatgtg caggttgaag tacccaaatt cacccggacg gacggacgga tacacactgg
     1801 catcatattc aaaaccaaag tcaagtaaat gacctaaccc atttttatca atacgcccaa
     1861 taggggctcg atatccaagc ggctttgtac cgcgaaattt tttgtaggca acttgtgagt
     1921 tttgcacttc atctaatgtg caggcatta
//
LOCUS       DAOREF010000663         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48114_length_1949_cov_3.421859, whole genome shotgun sequence.
ACCESSION   DAOREF010000663 DAOREF010000000
VERSION     DAOREF010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48114_length_1949_cov_3.421859"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..730)
                     /locus_tag="PKI33_12745"
     CDS             complement(131..730)
                     /locus_tag="PKI33_12745"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNM37929.1"
                     /translation="MTNWAQRVTLQGKYVRLEPMGEQHISGLAEIGAGQDFWSFMLYG
                     DMKTEADMRNWVLDILEREKKGGDLPFVVIHLASGKVAGATRYLNIIPKDRGLEVGGT
                     WYGKEFQRTAVNTECKYLLLTHAFEVLKTIRVQIKTDSINLRSQAAIERIGAVKEGVL
                     RNHMILPDGRIRHSVFYSILDSEWVGVKKNLEAMLKKYE"
     gene            complement(727..1188)
                     /locus_tag="PKI33_12750"
     CDS             complement(727..1188)
                     /locus_tag="PKI33_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNM37930.1"
                     /translation="MIHYLEREQVIPGTMKDIWEFFCNPYNLNTITPPDMNFKIIKGG
                     DRKMHPGQMIEYQVEFIRGIRSLWLTEITHVREGEFFVDEQRIGPYRLWHHEHIFESV
                     NGGVKMADRVTYMLPFELLGDIVHAVWVRRRLDSIFDFRTQKTTELFGNLK"
     gene            complement(1185..>1949)
                     /locus_tag="PKI33_12755"
     CDS             complement(1185..>1949)
                     /locus_tag="PKI33_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNM37931.1"
                     /translation="GSDIRGMNIEYSPSGIHFTAVAKDIRVAVSSNLVGAYNVSNCLA
                     ALTAAVYGLGIDSETASKGIASLEGIPGRMERIDMGQNFTAIVDFAHTPNALKVALEA
                     GRTMTDKRVIAVFGSAGLRDKEKRRMMAETSAELADLTVLTAEDPRIESLNGILEEMA
                     NGMKSKGGREGVNFWRVPDRGEAIKFALTLAQPGDIVLSCGKGHEQSMCFGKTEYLWD
                     DRTAMRAALADYLEVQGPAMPYLPSQDTPEEEWLKG"
BASE COUNT          525 a          521 c          382 g          521 t
ORIGIN      
        1 atgagcatat gcgtttgcac ggcatagaat tcatggaaga gcctcgccat gaagcctatg
       61 gaaccgtcgc cgtattcaaa gatttatatg gaaacaagtg ggatttgcta caaccaaaat
      121 aaaaatattg ctattcgtat tttttcaaca tggcttccag gtttttcttt acccctaccc
      181 attccgaatc caagatcgaa tagaatactg aatgccgaat gcgcccatcc ggcaaaatca
      241 tgtgattacg caagactcct tctttcaccg cgccaatgcg ttcaatggca gcctgagaac
      301 ggaggttgat cgagtctgtt ttgatctgta cacgaatggt tttaagaact tcaaaagcat
      361 gggttagcaa gaggtatttg cattctgtat tcacagccgt acgttggaat tcttttccat
      421 accaggtgcc acctacttca agtccgcgat ctttgggtat gatattcagg taacgagttg
      481 cacctgccac cttgccagat gcaagatgaa taacgacaaa agggagatca ccaccttttt
      541 tctcgcgctc cagaatatcc aatacccaat ttcgcatatc ggcttcggtc ttcatatctc
      601 catacaacat gaagctccaa aaatcctgcc cagcaccgat ctctgccaac cccgatatat
      661 gttgttcgcc catcggttcg agacgcacat atttcccttg aagcgtgaca cgctgagccc
      721 aatttgtcat ttcaggttcc caaataactc ggtagtcttt tgggtgcgaa agtcaaagat
      781 tgaatctaac cgtcttctca cccaaaccgc atggacaata tctcccaaca actcaaacgg
      841 caacatatac gtcacacggt cggccatctt aactccacca ttcacactct caaagatgtg
      901 ttcatgatgc cacaaccgat atggacctat tcgttgttca tccacaaaaa actcaccttc
      961 gcgaacatgc gttatctccg tcagccataa cgaacgaatg ccacggataa actccacctg
     1021 atattcgatc atctgcccgg ggtgcatttt tctatctccg cccttaataa tcttaaagtt
     1081 catatcaggc ggagtaatcg tattcagatt gtatgggtta caaaaaaatt cccagatatc
     1141 tttcatggtt ccgggaatta cttgctctcg ttctaaataa tgaatcatcc cttcaaccat
     1201 tcttcttctg gtgtatcttg cgaaggaaga taaggcatgg caggtccctg cacctcaaga
     1261 taatccgcaa gagcggcacg catggcagtg cgatcatccc ataaatactc ggtcttgcca
     1321 aagcacatcg actgttcgtg cccctttcca caggaaagga caatatcgcc tggttgggca
     1381 agtgtcagcg caaacttgat agcttcgcca cgatctggca cacgccagaa gttcacacct
     1441 tcgcgtccgc ccttggactt cataccgttt gccatttctt caaggattcc attcagcgac
     1501 tcaatgcgtg ggtcttcagc agttagcacc gttaagtcag ctaactctgc agaagtctct
     1561 gccatcatac ggcgcttttc cttatcacga agccctgccg aaccaaagac agcgatgaca
     1621 cgtttatctg tcatcgttct gccggcttct aatgcaactt tcaatgcatt aggtgtgtga
     1681 gcaaaatcca caatggcagt gaagttctgc cccatatcaa tgcgctccat acggcctgga
     1741 atcccttcta gagaagcaat acctttggag gctgtttcag agtctatacc aagaccgtaa
     1801 acagccgcag ttagcgcggc aaggcaattt gagacgttat atgccccaac caaattactt
     1861 gataccgcta cacgaatatc ttttgctacc gccgtaaagt ggattccaga tgggctatat
     1921 tcgatattca tcccgcgaat atcgctccc
//
LOCUS       DAOREF010000664         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48242_length_1946_cov_3.996298, whole genome shotgun sequence.
ACCESSION   DAOREF010000664 DAOREF010000000
VERSION     DAOREF010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48242_length_1946_cov_3.996298"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="PKI33_12760"
     CDS             <1..1646
                     /locus_tag="PKI33_12760"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNM37932.1"
                     /translation="IDQLPRDTECIITDFGLVRLTNSTTQTASGVVSGTPAYMSPEQA
                     QGSTVDSRSDTYSLGIVLYEMLAGRLPFDGDSTLGVILKHISEPPPPIEDISPEIQAV
                     VNKALEKLPENRYQTARDLLMDFYNAVGMHAEAETLYSLRLRTPPPTATLSNKPAPKP
                     NRFLWIGAGILACLCLGVIATGAVGFSIYSLFPKATTTPQILTDAATPDHMPHGNTAE
                     PGSTPQPISFGEFFGVLRFQGNMDQVTVTASLPDPAEGTQYEAWLIDDGGELLISLGI
                     LEKNNSGQYNLTFTDPESRNLLDGFNRMEITLETNPDASPNPSDTILYSSGIPAGALT
                     HVRHLLVSFPSNPNQTPMTGGLMETSSLINDAGTALEKAFEKGDTTAIRLQAETLINL
                     IVGKQSNEYKDWDKSGKISDPSDGFGLLLNGGQTGYIEGTITHAELSASAGDATANIK
                     LHSEHVVISAKNVEGWATQLRDVAKRILESDQVSESDIRLAVSLAEQIYQGVDIDGNE
                     SVDPVEGEGGALTAYQHAEYMADMQVLEGANLFPPPGNP"
     gene            1766..>1946
                     /locus_tag="PKI33_12765"
     CDS             1766..>1946
                     /locus_tag="PKI33_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37933.1"
                     /translation="MTIVTAETFVQAPLKFAYRAFTNATSLREWLCDVATVAPHPRGR
                     MYLWWAGDFYSSGHYL"
BASE COUNT          499 a          526 c          462 g          459 t
ORIGIN      
        1 ccatcgacca acttccgcgt gataccgaat gcatcatcac agactttggt ttggtaaggc
       61 tcacaaactc cacaactcaa accgcgtcag gggttgtcag tggcacgccg gcctatatgt
      121 caccagagca ggcacaaggc tccacggtag atagtcgctc cgacacctat tcccttggca
      181 tcgttttata tgaaatgttg gcgggacgtt tgcctttcga tggcgactct actttgggcg
      241 tgattctcaa acacatctct gaaccgccgc cacccatcga agacatctca cctgaaattc
      301 aagccgttgt caacaaagca cttgaaaaac ttcctgaaaa ccgatatcaa accgcgcgtg
      361 accttttgat ggatttttac aacgcagtgg gcatgcatgc agaagccgag actttatatt
      421 ccctgcgcct ccgcaccccg ccgccaactg ccactcttag caataagcct gctcctaaac
      481 caaaccgctt cctttggatc ggtgccggaa ttctggcatg tttatgttta ggggttatcg
      541 ccacaggtgc agtcggattt tccatttatt cattgttccc caaagcgacc actacaccac
      601 aaattctcac cgatgccgcc acgccagacc acatgcctca tggcaacaca gcagaacccg
      661 gctccacgcc tcagcccatc tcctttggcg aattttttgg tgtattacgc ttccaaggca
      721 acatggacca agtcacagtc accgcctccc tgccagaccc ggcagaaggc acgcaatatg
      781 aagcctggct gatcgacgat ggcggcgaat tactcatcag ccttggaatc ttggaaaaga
      841 ataattccgg tcaatacaat ctgacgttta ctgaccctga aagccgcaat ttattagacg
      901 gtttcaatcg catggaaatc acgctcgaaa ccaatccaga tgccagcccc aacccatccg
      961 acacgatctt gtattcctct ggcatccccg ccggtgcgtt gactcacgtt cggcatttgt
     1021 tagtcagttt ccccagcaac cccaaccaga cccccatgac aggcgggttg atggaaacca
     1081 gcagtctgat caatgatgct gggacagcgc tggagaaagc ctttgaaaaa ggcgatacga
     1141 ctgcaattcg cttacaagcc gagacgttga tcaaccttat tgtgggcaag caaagcaacg
     1201 aatacaaaga ctgggacaag agcggaaaga tcagtgaccc tagcgatggc tttgggcttt
     1261 tgttgaacgg tggtcagacc ggttatattg aagggacgat cacccatgcc gaactttctg
     1321 cttcggcggg cgatgccact gcaaatatca aacttcacag tgagcatgtc gtgatcagcg
     1381 cgaaaaatgt tgagggatgg gcaacccagc ttcgagatgt cgcgaagcgg attctcgagt
     1441 cagaccaggt ctctgagtcc gatatccgct tggcagtttc acttgcagaa cagatttatc
     1501 agggtgtgga tattgatggc aacgaaagtg tggacccggt tgaaggggaa ggcggggcat
     1561 tgacggcata tcaacatgca gaatatatgg cagatatgca ggtccttgaa ggcgcaaatc
     1621 ttttcccacc accggggaac ccgtagagta aacgtcgtgc ctttcgcacg acgtttttat
     1681 ttcaatagaa aacccatgcc agagtaggtt gaatcttggt ggcaatcgtt atataaagaa
     1741 taaatactta ctcgaggaga gtttcatgac tatagtaact gccgagacct ttgtacaagc
     1801 ccctttgaaa tttgcctacc gagcctttac gaacgcaacc tctctgcgcg aatggttgtg
     1861 tgatgttgcc accgtcgctc cacacccacg cggacggatg tatctgtggt gggctggcga
     1921 tttctattcc agcgggcatt atcttg
//
LOCUS       DAOREF010000665         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48255_length_1946_cov_2.781597, whole genome shotgun sequence.
ACCESSION   DAOREF010000665 DAOREF010000000
VERSION     DAOREF010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48255_length_1946_cov_2.781597"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKI33_12770"
     CDS             <1..1065
                     /locus_tag="PKI33_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNM37934.1"
                     /translation="VDCPSGIDCDTGECADETLHADLTVTMAAVKQGLLKLPAYEYIG
                     KLELVDIGLPKDLDSWDALQIDVADADMVSATLPKRTPASHKGTFGTAFVVAGSTSYT
                     GAALLAGSAAYRIGAGLVTMAIPAPLHTALVGHLPEATWVPLPHEHGFISEEASYYVL
                     SNLERATSILIGPGFGNKHTTKLFMEHLLDSLELPSVIDADGLRHLAGIHDWYKKIKV
                     QAILTPHPGEMSALTGLSISEIQSNRQEVALKYAKAWGHVVVLKGAFTVIASPSGRIT
                     IIPVATAALARAGTGDVLAGLIAGLRAQGVEAYEAAIAGAYIHAQAGLYAADRLGTTA
                     SVLASDVLEAIPRVISELEQ"
     gene            complement(1119..>1946)
                     /locus_tag="PKI33_12775"
     CDS             complement(1119..>1946)
                     /locus_tag="PKI33_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNM37935.1"
                     /translation="FIGKAFNDRTPFPAEVYFVSKKEFANKKWGTSQPILVETPGKGL
                     GYLFIQGFGTFSFQVKDPQQFVTQIVGATGTYRTSEIEERLKTMLVSKLTDVLGEFSQ
                     TTQSLAAKISGMNEELGAAVRAKGQADFEAIGLTLKTFYIGNLKPSTKSAQELRDAGL
                     LDPALYAQLQAADAMREAANNPNGGAGLTAGIGAGMGIGNLMGQALQGGMQTGGAPAA
                     GGGSKMPDIMTPAEAAQFMKVTEDDIMAAIQDGSLKAKKVGKAFRISKDNLESFMNS"
BASE COUNT          470 a          443 c          498 g          535 t
ORIGIN      
        1 gttgattgtc catctggcat tgattgtgat actggtgagt gtgccgatga aactttgcat
       61 gcagacctta cagttacgat ggcagcagta aaacaaggct tgctcaaact ccctgcatat
      121 gagtacatcg gtaaattaga acttgtggat attgggttac ccaaagattt ggattcttgg
      181 gatgctttgc agattgatgt cgcagatgct gatatggttt cagccacctt acccaagcga
      241 acgcccgctt ctcacaaagg gacatttggt actgccttcg tggtggctgg ctctacatcc
      301 tatacaggcg cggctttgct ggctggcagt gctgcctatc gtattggtgc aggattggtc
      361 acaatggcaa ttcccgcacc gcttcatact gcgcttgttg gtcatctccc cgaagcgact
      421 tgggttccat tgccacatga acatggattt atttcagagg aagccagcta ttatgttttg
      481 tcgaatttgg agcgtgcaac ctcaattttg atcgggccag gttttgggaa caagcacacg
      541 acaaaattgt ttatggaaca tttattggat tctctggaat tgccatctgt tattgacgca
      601 gacggactgc ggcaccttgc tggaattcat gattggtata aaaaaataaa agtgcaggct
      661 atcctaactc cacatcctgg cgagatgtct gcgctaactg ggttatcaat ttcagagata
      721 caatctaatc ggcaagaggt cgctttaaag tatgcaaagg catggggaca tgttgttgta
      781 ttgaaaggcg catttacggt tattgcttcc ccaagtggac gtataaccat aatcccagtt
      841 gctacagcag cccttgcccg agcagggacg ggggatgtgt tggcaggttt aatagccggt
      901 cttcgtgcac aaggtgtaga agcatacgaa gcggctattg cgggggcata tattcatgca
      961 caagctgggc tatatgctgc tgatagactt ggaacaacag cttcggttct tgccagtgat
     1021 gtgttagagg caattccaag agtcatctct gagttggaac agtagaataa aaaaaaagag
     1081 gctttcgtaa tgaaagcctc tttttttttc cttataaact agctattcat aaaagattca
     1141 aggttatcct tgctaatgcg gaacgcctta cctactttct tggctttcag gcttccgtct
     1201 tgaatggctg ccatgatgtc atcttcggtg accttcatga actgagctgc ttcagcagga
     1261 gtcatgatat caggcatttt tgatccacca ccagcggcgg gagcgccacc agtttgcata
     1321 ccaccctgaa gggcttgtcc catcaggtta ccaatgccca ttccggcgcc aataccagcg
     1381 gtgagaccag ctccaccatt cgggttgttt gcggcttcgc gcatggcatc tgcagcttgc
     1441 agttgagcgt aaagagcggg gtcgagcaaa ccagcatcgc gcaattcctg agcagacttg
     1501 gtgctaggct tgaggttgcc aatgtagaaa gtcttgagtg ttaagccaat ggcttcaaaa
     1561 tcagcctgcc ccttagcgcg gacagccgca ccgagttcct cgttcatgcc gctgatttta
     1621 gcagcaaggc tctgggtggt ctggctgaat tcaccgagca cgtcggtcaa cttggagacc
     1681 agcatagtct taagacgttc ttcgatttca gatgtgcgat atgttcctgt tgcaccaacg
     1741 atttgtgtca caaattgttg cgggtctttc acctggaagg agaaagtccc aaagccttga
     1801 atgaacaagt agcccaagcc tttgcccgga gtttccacga ggatgggttg tgaggtgccc
     1861 catttcttat ttgcgaattc cttctttgag acgaaataaa cttcggcggg gaagggggtg
     1921 cggtcattaa acgccttgcc aatgaa
//
LOCUS       DAOREF010000666         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48302_length_1945_cov_3.348677, whole genome shotgun sequence.
ACCESSION   DAOREF010000666 DAOREF010000000
VERSION     DAOREF010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48302_length_1945_cov_3.348677"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKI33_12780"
     CDS             complement(<1..592)
                     /locus_tag="PKI33_12780"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNM37936.1"
                     /translation="MSETVDYKSELDETQRALREITLMIEQSQGELSKLTQRNTAITS
                     HLQQVQKQGAAMEEIKMAYDSALDAQQRLFVMRGQLEKLQNDKTHLEKYKSVLDQFVN
                     DGNTTDVSGSAASSNNKGQMTGIEMIVNAQEAERQRLSRQMHDGPAQALSNFILQTEI
                     AMRLLDVDSSQAKEELSNLKAAAMSTFQKVRGFIFEL"
     gene            complement(602..1297)
                     /locus_tag="PKI33_12785"
     CDS             complement(602..1297)
                     /locus_tag="PKI33_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM37937.1"
                     /translation="MNQITLLVVDDHPLFRQGVVDALSLETDMRIIAQSASGDEALEF
                     IRTKKPNVAVLDVNLPGMNGQQITHQVSQDKMNTRIILMTGYDDIEQAIHAALAGAHG
                     YCSKDIEPQNLARIIREVAEGKFVFANNVFTRRELELWVEESMEGARRSYSEPGAPFH
                     PLSDREMEVLACVVRGMSNKEIASLLGISHQTVKNHVTAILRKFGVEDRTQAVVYALK
                     RGWVTLRDTDIRS"
     gene            complement(1364..>1945)
                     /locus_tag="PKI33_12790"
     CDS             complement(1364..>1945)
                     /locus_tag="PKI33_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37938.1"
                     /translation="AWSVGYMIIYLLRLPVYQHGRYIIPAMPIFFLIGLLGMFSYVTV
                     KREGRNFWMISTSWNLSLAMVTLLFIVLGARAYARDVAFIEQEMVAAAKWAAANIPPD
                     AILAVHDIGAMGYFDEHQLIDLAGLITPEVIPFIRDEDRLAAYLDDKGADYLVAFADL
                     YPQMEVGREVVFVTNGAIATEADWVRMTIYRWK"
BASE COUNT          531 a          474 c          408 g          532 t
ORIGIN      
        1 gaagttcaaa gataaaaccc cgtactttct ggaatgtact catagcagca gcttttagat
       61 tcgacaattc ctctttcgcc tgagaagagt cgacatccaa caaacgcatc gcaatttcag
      121 tctgaaggat aaaattggat aaggcttgag cgggtccatc atgcatttga cgagacaagc
      181 gctgacgttc agcctcttgt gcgttgacaa tcatttcaat gcctgtcatc tgtcctttat
      241 tgttagatga agctgcggag cctgaaacgt cagtagtgtt accgtcattt acaaattggt
      301 ctaaaacaga cttgtatttc tcaagatgtg ttttatcgtt ttgaagtttt tctaattgtc
      361 cacgcataac gaacaagcgt tgttgagcat ccaatgcaga atcgtaagcc atcttaatct
      421 cttccattgc agcgccctgt ttctggactt gctgaagatg agatgtgata gcagtattcc
      481 gctgtgttag tttgcttaac tcgccttggc tttgctcaat catcaaagtg atttcacgca
      541 aagcgcgctg cgtttcgtct aattcgcttt tatagtccac agtttcggac atctaattct
      601 cctaagagcg gatatcggta tcccgcaggg taacccaacc gcgttttaat gcgtacacaa
      661 cagcttgagt acggtcttcc accccaaact tacgtaaaat tgctgtgaca tggttcttca
      721 ctgtttggtg actgataccc aacagcgagg caatttcctt gttgctcata ccgcgtacca
      781 cacaagccaa gacttccatt tcacgatcag aaagcggatg gaatggcgca ccaggttcac
      841 tataggaacg gcgggcacct tccatgcttt cttccaccca taattcaagt tcacgacgag
      901 taaaaacatt attcgcaaat acaaatttac cttcagctac ttcgcgaatg atgcgagcca
      961 agttctgagg ctcgatatct ttagagcaat atccgtgggc accagccaat gcggcatgga
     1021 tcgcttgttc tatatcatcg tacccggtca tcaaaatgat gcgggtgttc atcttatctt
     1081 gcgagacctg atgtgtaatc tgctgaccat tcatgcctgg aaggttcaca tctaaaacgg
     1141 caacattggg ctttttggtg cggataaact ccagcgcctc atccccagac gcagactgag
     1201 cgatgatccg catatctgtt tctaatgaca gagcatccac cacgccctgg cggaataatg
     1261 ggtgatcatc aacgactagt aatgtaattt ggttcatcgc ttagcattct ctctttttag
     1321 aaggttttca atcctaacat tcgccatatt ttaacccatt ctttcacttc caacggtaaa
     1381 ttgtcatgcg aacccaatct gcttctgtag caattgcccc attggttaca aaaaccactt
     1441 ccctaccgac ctccatctgg gggtaaaggt ctgcgaaagc aacaagatag tctgcaccct
     1501 tatcatcaag gtaggcggcg aggcggtctt catcacggat aaagggaatg acttctgggg
     1561 tgatcaaacc agccaagtct atgagttggt gttcatcaaa ataacccatg gcaccgatat
     1621 catgaacggc aaggatcgca tcgggcggga tgttagcggc tgcccatttt gcagcggcga
     1681 ccatttcctg ctcgataaag gcaacgtctc ttgcgtatgc tcttgctcct aagacaataa
     1741 ataataacgt caccatagca agactcaaat tccaggatgt agaaatcatc cagaagtttc
     1801 gcccctctct cttaacggtg acgtaactaa acattcctaa aagtccgatc aagaagaaga
     1861 ttggcatagc agggatgata taacgcccgt gttgataaac aggaaggcgc agaagataaa
     1921 taatcatata ccctacagac caagc
//
LOCUS       DAOREF010000667         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48382_length_1943_cov_4.509004, whole genome shotgun sequence.
ACCESSION   DAOREF010000667 DAOREF010000000
VERSION     DAOREF010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48382_length_1943_cov_4.509004"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PKI33_12795"
     CDS             <1..823
                     /locus_tag="PKI33_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNM37939.1"
                     /translation="FFFNAHNNLLEEVAKFRAARRMWARIMRERFKAQKPASWQLRFH
                     TQTAGSTLTAQQPENNVVRVTLQALSAVLGGTQSLHTNSMDEALWLPTEKSVRVALRT
                     QQIIAYESGVADSIDPLAGSYLIEQMTDEIEQGALDYIARIDEMGGAMQAIERGYMQN
                     EIQNAAYAAQQAIEKKEDIVVGVNAFEVKEERNLERLVVDPAIELGQREKLKKLRENR
                     DPKAVEQLRARLVTAAQGTGNLMPLFIECVENDMTLGEICNILRGVWGEYVAEGF"
     gene            1039..>1943
                     /locus_tag="PKI33_12800"
     CDS             1039..>1943
                     /locus_tag="PKI33_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37940.1"
                     /translation="MGLVSEVFDLLISGVDFAGKVLSAIISRPDQPKNPEPQKIESKE
                     ITTTTTFEKEIHHHHEPQVIEREIRTEPKIIEKEIHHKPTIIENSEPKIIEKEIHHEP
                     ITIYEPVKIVEKRVLDKKFLTDVVDKTVEKLSILLNKQTVQFFYILEEHQIRGAIQEL
                     QARVSALKTLVNYTTLDPNIATQIMVSSLNPLQVSLNIVEIRLKDAGQNELWEYCYVV
                     GNSALLTGYAYLGQDLPALREELEDHLKIIQNKLINQVAREIIISGGEIPWNEVPSML
                     KPENAYKILDLNKSYQQSLQMPFDL"
BASE COUNT          585 a          397 c          504 g          457 t
ORIGIN      
        1 cttcttcttc aatgcgcaca acaacctgct ggaagaggtg gcgaagttcc gcgcggcaag
       61 gcggatgtgg gcgcggatca tgcgcgagcg cttcaaggcg cagaagcctg cttcgtggca
      121 gttgcgcttc catacccaga cggccggttc gaccctgacc gcgcagcagc cggaaaataa
      181 tgtggtgcgt gtgaccctgc aggcgctttc tgccgtgctc ggcgggaccc aatccctgca
      241 caccaacagc atggatgagg ctttgtggct gccgacggag aagtcggtgc gggtggcgct
      301 gcggacccag cagatcattg cgtatgaatc cggcgtggca gattcgattg acccgctggc
      361 aggttcgtat ttgatcgagc aaatgactga tgagatcgag cagggcgcgt tggattacat
      421 cgccaggatc gatgaaatgg gcggggccat gcaggccatt gagcgcgggt atatgcagaa
      481 cgagatccag aacgcggcgt atgcggctca gcaggcgatc gagaagaaag aagacattgt
      541 tgtgggcgtg aatgcctttg aggtgaagga ggaaaggaac ctggagaggc tggtggtgga
      601 ccccgccatc gagttgggtc agcgcgagaa gttgaagaaa ttaagagaga atagggaccc
      661 aaaagccgtg gagcagttgc gcgcacggtt ggtgacagcc gcccaaggga ccgggaattt
      721 gatgccgctc ttcatcgaat gtgtggagaa cgatatgacg ctgggcgaga tctgcaatat
      781 cttgcggggt gtttggggtg agtacgtggc agaagggttt taggcgttcg ggccacaaag
      841 gcatggaaat ttgccctttg tggccttttt cttgccctgt ccttggggcg gttgtataat
      901 caatttatgg agtctatagg gtgcttatac agcatgatgc tgtataacac cccaaattgg
      961 gggtatatac agcgcaacgc tgtatatacc atagttgggc agttccttgc aaaataatac
     1021 tcatataagg agaataaaat gggactcgta tctgaagtgt ttgatttatt gatttcagga
     1081 gttgattttg ctgggaaagt tttaagtgcc ataatttctc gtcctgacca gcccaaaaat
     1141 cctgaaccgc aaaaaataga gtcgaaagaa ataacaacga ccactacttt tgagaaagaa
     1201 atacaccatc accacgagcc acaggtgatt gaaagagaga ttcgcactga gccaaaaata
     1261 attgaaaaag aaattcacca taagcctacg attattgaaa acagcgagcc aaagataatc
     1321 gagaaagaaa tccatcacga accaataaca atttatgagc cagtaaaaat tgttgaaaag
     1381 cgagtgctgg acaagaaatt tttaacagat gtagtggata aaactgtaga aaaactatct
     1441 attctattaa ataagcaaac agttcaattt ttttacattt tagaagaaca tcaaattcgc
     1501 ggcgcaattc aagaattgca agccagagta agcgcattga aaacgttggt aaattacaca
     1561 acgttagacc cgaacattgc tactcaaatt atggtatctt cattaaaccc tctccaagtc
     1621 agtttgaaca ttgtagagat tcgattgaaa gacgctggac aaaatgaact ttgggaatat
     1681 tgctatgtag ttggcaatag cgctctgctt actggatacg cctatttagg acaggacctt
     1741 cctgccttaa gagaagaatt ggaagaccac ttaaaaataa tccaaaacaa attaatcaat
     1801 caagtagcac gagaaataat tatcagcggt ggagaaatcc cctggaatga agtaccgtct
     1861 atgttaaaac ctgaaaatgc ctataaaatc cttgacctta ataaatccta tcagcaaagt
     1921 ttgcaaatgc cttttgactt aaa
//
LOCUS       DAOREF010000668         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48486_length_1941_cov_4.269353, whole genome shotgun sequence.
ACCESSION   DAOREF010000668 DAOREF010000000
VERSION     DAOREF010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48486_length_1941_cov_4.269353"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKI33_12805"
     CDS             <1..218
                     /locus_tag="PKI33_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNM37941.1"
                     /translation="KHLTNMRASRADMEIRLTPPRQMSLDEAIEYLADDELLEVTPAT
                     FRIRKRILNTEERGKQTKKAKEQFGEE"
     gene            complement(265..966)
                     /locus_tag="PKI33_12810"
     CDS             complement(265..966)
                     /locus_tag="PKI33_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNM37942.1"
                     /translation="MKRSIYLFLITSLLLTSCSMSSSDAPSPEVATAAAMTVQAAIEG
                     NQTPLASPTSAGQSTPESTGNPMASFEDVTNCRVGPGVNYERVTQIQPGFAVEIVGYY
                     PPNYWIVQTDKGPCWVAGEFVTPSGSVSAVPTVTAPPTPTGGAPENVSLQKWDVACDY
                     ATNEAKVTIAWKDEDDEIGYRVVRNDVVIAELPANTTEFKETITLISGQTAGYSIISF
                     NATGSTQSQTISLGC"
     gene            complement(991..>1941)
                     /locus_tag="PKI33_12815"
     CDS             complement(991..>1941)
                     /locus_tag="PKI33_12815"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNM37943.1"
                     /translation="RHRAFKTAPTPLGADPVSGRIPLLGNNDVILGVSRAPKGKMNFF
                     YRNSQAYETWWVHDGSGTIYSQFGKLPFRKGDYIVIPFGTTWQMELDEETKFFTIENP
                     SQIEPPKRYRNHYGQLLEHAPYCERDIRIPMELETHTERGEFEVRVKVRDHISQHILD
                     YHPHDVIGWDGYLYPWIFNIEDFEPITGRIHQPPPVHQTFEGWNFVVCSFVPRLFDYH
                     PEGIPAPYNHSNIQSDEVIYYAEGNFMSRKGIDRSDISLHPFGLPHGPQPGMTEASIG
                     KTETKELAVMVDTFHPLHLTKQALEMEKPYMETWLDGDGE"
BASE COUNT          498 a          433 c          481 g          529 t
ORIGIN      
        1 agaagcattt gacgaatatg cgcgcctcac gtgcggatat ggaaattcgc ctcactccgc
       61 cccgacaaat gtctctcgac gaagcgattg aatacctcgc tgacgatgaa ttgctcgaag
      121 tgacgcctgc tacattccgc attcgcaaac gcattttgaa caccgaagag cgcggcaagc
      181 aaacgaagaa ggcaaaagaa cagttcggcg aagaataaaa ataaaagagc catgcaattt
      241 gcatggctct ttttgattca caaactaaca gcctagtgag attgtctgac tttgcgtaga
      301 ccctgttgca ttgaaggaga taatgctata cccagctgtt tgacctgaga ttagcgtgat
      361 ggtctcttta aactctgttg tatttgcggg taactcagcg atcacaacat cattgcgaac
      421 gacgcgatag ccaatttcat catcttcatc tttccacgca atcgttactt tggcttcatt
      481 tgtagcgtaa tcacaagcga catcccattt ttgcaagctg acattttctg gtgctccacc
      541 ggtgggtgtt ggtggtgccg taaccgttgg aaccgcgcta acactccctg atggggtgac
      601 gaattcgcca gctacccaac aaggtccttt gtctgtttga acgatccagt aattaggcgg
      661 gtaatagccc acgatttcta ccgcaaaccc tggctgaatc tgggtgacac gctcgtaatt
      721 tacaccgggt cccacacggc aattggtgac atcttcaaag cttgccatag ggtttcccgt
      781 gctttctggt gtggactgtc ctgcgctggt tgggctggca agtggggttt gattaccttc
      841 aatagcggct tgaactgtca tagcagcggc agttgctact tctggggaag gggcatcaga
      901 actagacatg ctacaagatg tgaggagcaa actggttatg aggaataaat aaattgatcg
      961 tttcattgta ttctccgatg taaattatct ttattcgccg tcaccatcta accaggtttc
     1021 catgtaaggc ttttccattt cgagagcttg tttggtcaaa tgaagcggat ggaaagtatc
     1081 gaccattacg gcgagctctt tcgtctctgt ctttccaatg ctggcttctg tcataccggg
     1141 ttgtggacca tgtggcaaac caaacggatg caaactgata tcagaccggt ctatgccttt
     1201 gcgagacatg aaattgcctt ctgcatagta gataacttcg tcagattgga tgttggaatg
     1261 attgtatggc gcaggaatgc cttcagggtg ataatcaaag agacgtggca caaaagaaca
     1321 tacaacgaaa ttccagcctt caaaggtttg gtgaactggc ggtggttgat gtatacgccc
     1381 ggtaataggc tcgaaatctt caatgttgaa aatccaaggg tataagtatc catcccagcc
     1441 aatgacatca tgaggatgat agtctaggat atgttgcgag atgtggtcgc gtactttgac
     1501 acgcacttca aactcaccac gttcggtgtg agtttctaac tccatgggga tgcggatatc
     1561 acgctcacag taaggggcat gttcgagcag ttgaccgtag tgattgcggt agcgcttggg
     1621 cggctcgatc tggctagggt tttcgatggt gaagaacttt gtttcttcgt ctaattccat
     1681 ttgccaagtt gtgccaaatg gaatcacgat gtaatcgcct tttcgaaagg gtaatttgcc
     1741 aaattgggaa tatatggtgc cgctaccatc atgaacccac caagtttcat aagcttgtga
     1801 gttgcggtag aaaaagttca tttttccttt aggagcccgt gagactccca agataacatc
     1861 gttatttccg agcagaggaa ttcttccaga aactggatct gccccgagcg gagtgggagc
     1921 cgttttgaac gcacgatgac g
//
LOCUS       DAOREF010000669         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48640_length_1938_cov_2.561869, whole genome shotgun sequence.
ACCESSION   DAOREF010000669 DAOREF010000000
VERSION     DAOREF010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48640_length_1938_cov_2.561869"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1387
                     /locus_tag="PKI33_12820"
     CDS             170..1387
                     /locus_tag="PKI33_12820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM37944.1"
                     /translation="MTESKITLYGAYWCPDCRRSKKFLGEQFVPFKWIDIEQDKEAEA
                     LVLQKNNGKRIIPTIVFEDGSFLVEPTNAELAKKLGLKTEAKKTYYDLIIIGGGPAGL
                     TAAIYASREGADVLLIERSGLGGQAGITVGLDNFPGFPEGISGQEFSDRVTQQAKRFG
                     VEILQAVDVERLEEENGYHEVYTSDGKHYHSRAVLLATGASYRRLEVPGEDDYIGAGI
                     HFCATCDGPFYKGAKEIVVIGGGNAAVEEGLHLTNFAEKVTLLARGDKLTASQIAIDK
                     VTEPGSKVDVRYNVSVEGFDGQDSKLKSIKTRNKVSGQVEELHPAAAFVFIGQQPNTE
                     FVKSYVQTDPSGFILTGHDLGHDQDALPFETSVRGIFAAGDARHGSVKQVASAVGEGA
                     AVSISIREFLRKG"
     gene            complement(1389..>1938)
                     /locus_tag="PKI33_12825"
     CDS             complement(1389..>1938)
                     /locus_tag="PKI33_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM37945.1"
                     /translation="CVEKTIEAFGRLDILFNNAGIIHVNRNVVTTLEDEWDATLNSNL
                     KGTYLMSKYAIPYMEKQGGAIVNNASIFGLVGGGGVAAYCAAKGGVINLTRAMAIDHA
                     AQNIRVNCVCPGSVDTPLLEKEMDDLGGADLQRPKFASRHPMNRIATPEEVANAVAYL
                     ASDAASFVTGTALPVDGGRAAW"
BASE COUNT          507 a          450 c          517 g          464 t
ORIGIN      
        1 tttgatgtgc attcatgggt tgaaatgcct tcctgttttg aattttaggt gtaggctttg
       61 tttgaagccg tttttttaga attttatcat ctagagtagg aagattgtcc tagtaaagaa
      121 atgcaagtac atcacaccag tacagcaaat caatttaccg gaggaagaaa tgaccgaatc
      181 caagatcacc ctgtatggtg cctattggtg cccagactgc cgccgctcga agaaattttt
      241 gggcgaacaa tttgtcccct ttaagtggat cgatattgag caggacaaag aagccgaagc
      301 cctcgtgctt caaaagaaca acgggaagcg catcatccca accattgtgt ttgaagatgg
      361 gtcatttcta gtcgaaccga ccaacgccga gcttgccaaa aagctgggat tgaaaaccga
      421 agcaaagaaa acctattatg acctgatcat catcggtggc ggacctgccg ggttgaccgc
      481 cgcgatctac gcctcacgtg aaggtgcaga tgtgttgcta atcgaacgct ctggtttggg
      541 cgggcaagcg ggcatcacgg ttgggttaga taactttccc ggctttcctg aaggcattag
      601 cggacaagaa ttctcagatc gtgtcacgca acaagctaaa cgcttcggcg tagaaattct
      661 gcaagccgta gatgtggaac ggctggaaga agaaaatggc tatcacgaag tatatacatc
      721 agacggcaag cactatcatt cacgtgctgt cttgttagct acgggcgcat catatcgcag
      781 actggaagtg cccggtgagg atgattacat tggggcgggc attcatttct gtgccacgtg
      841 tgatggtccg ttttataaag gggcaaaaga aatcgtggtt atcggtggtg gaaatgccgc
      901 cgtggaggaa ggtttgcatc tgactaactt cgccgagaaa gtgaccctct tggcacgcgg
      961 agataaactg actgccagcc agatcgcgat tgacaaagtg accgaacccg gctcaaaagt
     1021 ggatgtgcga tacaacgtca gcgtagaagg gtttgatgga caggattcaa aactaaagtc
     1081 cattaagaca cgtaataagg tgtccggtca ggtggaagag ttgcatccag cggcagcgtt
     1141 tgtgtttatc ggtcaacaac caaacactga gtttgtgaaa agctatgtgc agaccgaccc
     1201 aagcggattt attctgacgg gtcacgactt ggggcacgat caagatgctt tgccatttga
     1261 aaccagtgtt cgtggcatct ttgcggcagg cgatgcgcgg catggcagtg tcaaacaagt
     1321 ggcatcggca gtgggcgaag gcgcggcggt ttcgatctcg atccgcgagt ttttgcgcaa
     1381 aggttaattt accaggcagc cctgccgcca tccacaggca gggctgtgcc tgttacaaaa
     1441 gatgcagcgt ctgaggcgag atacgccaca gcgttggcaa cttcttcggg tgtagcaatc
     1501 cgattcatcg ggtggcgtga ggcgaacttg gggcgttgca ggtccgcgcc acccaaatca
     1561 tccatttctt tttcaagcaa aggcgtatcc acactgcccg ggcaaacgca attgacgcgg
     1621 atgttctgag cggcatggtc aattgccatg gcacgtgtta ggttaatcac accacctttc
     1681 gcggcacaat aagcagcgac tccgccgcca cccaccaagc caaagatcga agcattgttg
     1741 acaatcgcgc cgccttgttt ctccatatat ggaatggcat acttcgacat gagatatgtg
     1801 cctttcaaat tggaattcaa ggtggcatcc cattcatctt ccaaagtggt caccacattg
     1861 cggttgacat gaataatgcc tgcgttgttg aacagaatgt ccaaacggcc aaaggcttcg
     1921 atagttttct ctacacaa
//
LOCUS       DAOREF010000670         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48674_length_1937_cov_3.824123, whole genome shotgun sequence.
ACCESSION   DAOREF010000670 DAOREF010000000
VERSION     DAOREF010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48674_length_1937_cov_3.824123"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PKI33_12830"
     CDS             <1..751
                     /locus_tag="PKI33_12830"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNM37946.1"
                     /translation="DTQARALLEHEFENERLHEANELILEGRHEVIQFAGVFMQVNGL
                     TYVSGLPVTLPATIPLPANGDAVVVSGRTNSQGFVEVINLEFLPAGSVVPVGKPIEVE
                     TESDTEQATGQTPTPSTGTSSGSTNEATPTPVKEYEVKGTLEAVSTTSLVVTGMTVYL
                     ENSKIDGQMCVGVEVEVKGYYAQDGRFIVKEAKSKGSCTSINNSGSNSGSSNNESGSN
                     SSGESNSNGDDSGDDHGGNSGKDGGGGDDND"
     gene            901..>1937
                     /locus_tag="PKI33_12835"
     CDS             901..>1937
                     /locus_tag="PKI33_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNM37947.1"
                     /translation="MSDNTFYLGRLFDPKTAKLTSTDINYDSADLTTHAVVTGMTGSG
                     KTGLCISLLEEAALNGVPAIIIDPKGDLTNLLLHFPELLPKDFQPWIDPEVVRRANKT
                     PEQASQEAAMAWRNGLQEWGMTPERILDLKNSVDFAIYTPGSDSGIPVSVLSSLDAPG
                     LDWDSNREVLRERITSTVTALLGLVGMNDLDPIRSREHILLSNIFEEHWSQGNGIDLT
                     ELILQTQNPPFDKLGAFPVDTFFPPKDRMALAMTLNNILASPAFEAWREGQALDVQSL
                     LFKEDGTPRHSIFYLAHLSDGERMFFTTLLLSAVETWMRTQKGSAALRALLYMDEIYG
                     YLPPTANPPSKL"
BASE COUNT          499 a          477 c          461 g          500 t
ORIGIN      
        1 tgacacccaa gcccgcgctt tgttggaaca tgaatttgaa aatgaacgtt tgcatgaagc
       61 caatgaattg atccttgaag gacgtcacga agtgattcag tttgcgggtg ttttcatgca
      121 agtgaacggg ttgacatacg tctctggctt gcccgtgacc ttgcctgcta ctattcccct
      181 ccctgccaat ggagatgctg tggtcgtcag cggacgcacg aattcacaag gctttgtaga
      241 agttatcaac ttggagtttt tacccgccgg ctcggtcgtg ccggttggaa aaccaattga
      301 agtggaaacg gagtcagata cagaacaagc tacagggcaa accccaactc cttcaacagg
      361 taccagttct gggtccacga atgaggcgac acctactccg gtaaaggaat atgaagtcaa
      421 aggtacgctt gaagcggtat ctactacatc tctcgtcgtc actggtatga cggtgtatct
      481 tgaaaattcc aaaatcgatg ggcagatgtg tgtgggtgtt gaagtggaag tgaagggcta
      541 ttacgctcaa gacggtagat ttattgtgaa agaagcaaaa agtaaaggaa gttgtacgag
      601 tattaataac agtggttcaa attctggcag tagtaataat gagtctggtt cgaatagctc
      661 tggagaaagc aactcaaacg gcgatgactc aggggatgat catggtggga acagtggtaa
      721 agacggtggc ggcggcgacg ataacgatta agaccaaact gcgaggaaac ccctcgcagt
      781 ttttctttcg caatcatttg ccttttggca taaaatcggt taaattaaac atctagcacg
      841 ttcgttctaa tcaatacaac ttcaagactt caaaagtctt atatccatag gagagattat
      901 atgagcgaca acacctttta tcttgggcgt ctgtttgacc cgaaaactgc caagctcact
      961 tcaaccgata ttaactatga ctcagccgac ctgacaaccc atgcagttgt caccggtatg
     1021 accggttctg ggaaaacggg cttatgcatt tcgcttcttg aagaagcagc gctcaatggt
     1081 gtgcctgcca tcatcatcga ccccaaaggg gacctgacca atctgctcct gcattttcct
     1141 gaattattgc caaaagattt tcagccgtgg atcgatcccg aagttgtgcg ccgtgcaaac
     1201 aaaacacctg agcaagcctc gcaagaagct gccatggcgt ggcgcaatgg actgcaagaa
     1261 tgggggatga ctcccgaacg aattctcgac ctcaagaatt cagttgattt tgctatttat
     1321 acacccggct ctgactcggg tattcctgtc agtgtgcttt cgtcgctcga tgcccccggt
     1381 ttagattggg actccaaccg cgaagtgttg cgcgaacgca tcaccagcac agttacagcc
     1441 ttgctaggct tggtcggtat gaatgatctc gacccgatcc gctcgcgtga acatattttg
     1501 ctttcgaata tctttgaaga acattggtcg caaggcaatg gcatcgacct aaccgagctc
     1561 atcttgcaga cgcaaaaccc gcctttcgat aaattgggcg ccttcccagt cgatactttc
     1621 ttcccgccca aagaccgcat ggcattggcg atgaccctca acaacatcct tgcttcgcct
     1681 gcatttgaag catggcgtga aggtcaggca ttggatgtac agtccttgct cttcaaggaa
     1741 gatggcactc cacgtcacag cattttctac cttgctcatc tttccgatgg cgagcgtatg
     1801 ttcttcacaa ccttgttgct ctctgccgtt gaaacctgga tgcgcacaca aaaaggctcc
     1861 gctgctttgc gtgcccttct ttatatggat gagatctacg gctatcttcc tcccactgct
     1921 aatcctcctt ccaagct
//
LOCUS       DAOREF010000671         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_48770_length_1935_cov_3.149468, whole genome shotgun sequence.
ACCESSION   DAOREF010000671 DAOREF010000000
VERSION     DAOREF010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48770_length_1935_cov_3.149468"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..502)
                     /locus_tag="PKI33_12840"
     CDS             complement(20..502)
                     /locus_tag="PKI33_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37948.1"
                     /translation="MRKKWTIEQRVRGQNQRAKQAGARHDLMVEQWMETLEYFNYKCA
                     YCGERDYEFIEHYLPVRTAGTTVSNCVPACAMCNALKDAKNHKLTLYQTERVIEFLES
                     KGVKIAFHVHEYKATKEEYVTLVCEGCGDKLVFPGLSLDKAQDYIDEFLSNMGYAYRI
                     "
     gene            complement(480..1418)
                     /locus_tag="PKI33_12845"
     CDS             complement(480..1418)
                     /locus_tag="PKI33_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNM37949.1"
                     /translation="MELKTEIKRFLDTLDRSPRTISTYENALKQFMYVVGEDADLTPE
                     NYSAFLTVFKSASPSTKNTYGSAVRKFYKFCKAGHAEEIKEITNHLARNQGKRFVNFN
                     REAVEKVIEYCTQLSGDLEALRDRAFVLTLVDTGLRISEACALKRSDIDWMEGRAIII
                     GKGDKQAVVRFSKRSMEALKAYLHARSAVDPNSRIPLGSQPLFARHDIRASKKIRPIT
                     AGGMWKAIKGSPSDGIIGRIEEAGVDRSLVRIHDFRHYFVTMTYLAKGDLKLSQELAR
                     HESIATTNRYAHYGSEIDIAYDEIFNKRDNEKKMDN"
     gene            1678..>1935
                     /locus_tag="PKI33_12850"
     CDS             1678..>1935
                     /locus_tag="PKI33_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNM37950.1"
                     /translation="MPPIILAIIALTLSFGFLNGMHDSRNVVSTMISSRAYSPRVALG
                     VTILAELSGPFVFGVAVANSIGRGIATPAAINLQVILVALTA"
BASE COUNT          481 a          463 c          435 g          556 t
ORIGIN      
        1 atcagcgggg gaggggaggt tagatcctgt aagcatagcc catattactt aagaattcgt
       61 caatgtaatc ctgagccttg tcaagtgaca gtccagggaa aaccaattta tccccacatc
      121 cttcgcaaac caatgttaca tattcttcct ttgttgcttt gtattcatgg acgtgaaatg
      181 ctattttgac acctttactt tcaaggaatt ctataactcg ttccgtttga tacaaggtta
      241 atttgtggtt cttggcatcc ttaagtgcat tacacattgc acaagctgga acgcaattgc
      301 tgactgtagt tccagcggta cgaactggca aataatgttc aatgaattca tagtctcttt
      361 cgccacaata agcacatttg taattgaaat attccagtgt ttccatccat tgttctacca
      421 tgagatcatg ccgagcgccg gcttgtttag cacgttggtt ctgaccacgt acgcgttgtt
      481 caattgtcca tttttttctc attgtccctc ttattaaata tctcatcgta agcaatgtcg
      541 atttcgctac catagtgggc ataacgattt gtggtggcga tactttcatg gcgagcaagc
      601 tcttgcgata actttagatc gcctttggca aggtaggtca tggtgacaaa ataatgccgg
      661 aaatcatgaa tacggacaag gctgcggtca actccggctt cttcgatgcg accaataatt
      721 ccatcactag gggagccttt gatcgctttc cacatcccgc cagccgtgat ggggcgaatt
      781 ttcttgctgg cacgaatatc gtgtcgggca aataagggct gagaaccgag tgggatgcgg
      841 gaatttgggt cgacagcaga ccttgcgtgt agataagcct tcaaagcctc catggagcgc
      901 tttgagaagc ggactacagc ttgtttatcg cctttaccta taatgatggc acgaccttcc
      961 atccagtcaa tatcgctgcg tttgagcgca catgcttcag atatacgtaa gccagtgtct
     1021 accaaggtca aaacaaatgc acggtcgcgc aaggcttcca gatcaccact taactgtgtg
     1081 caatattcaa tgactttttc cacagcctcg cggttgaaat tgacgaagcg tttgccctga
     1141 ttccgcgcca gatggtttgt aatttccttg atctcttcgg cgtgaccggc tttgcaaaac
     1201 ttgtaaaatt ttcgcacagc cgagccgtat gtgttctttg tagaagggga tgctgacttg
     1261 aacacagtga gaaatgcaga ataattctct ggggtaaggt ctgcgtcttc accaaccaca
     1321 tacatgaatt gtttcagcgc attttcataa gttgaaattg tgcgtggaga gcggtctaaa
     1381 gtatctagga agcgttttat ctcggttttt agttccataa tcatcattcc atgtaataat
     1441 aattatatcg aatgatagat ataaagtcaa gagcggagcc ggaaatacaa catcccccac
     1501 caccctatac aaaatttttc cgcacatata catcacatac atcttagccg ggcagatggt
     1561 ctgcaaggca gtggaatatc aactcgtgca ttcatcctct cgcatttcct cgcccctccc
     1621 ctgccccaca aaaatggacc tgccagggtg taaaatagcg gacttggaca cacccacatg
     1681 ccgcctatta ttcttgccat tatcgctctg accctctcgt ttggctttct gaatggaatg
     1741 cacgactcgc gcaatgtggt ttcgaccatg atctcgtcgc gtgcctattc gccgcgagtt
     1801 gccctcggtg tgacgattct ggcagagtta tcgggtccct tcgtgtttgg ggtggcggta
     1861 gcaaactcga tcgggcgtgg aatcgctaca cccgcagcga tcaacttaca ggtcatattg
     1921 gtggcgttga ccgct
//
LOCUS       DAOREF010000672         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49022_length_1930_cov_3.993067, whole genome shotgun sequence.
ACCESSION   DAOREF010000672 DAOREF010000000
VERSION     DAOREF010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49022_length_1930_cov_3.993067"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PKI33_12855"
     CDS             complement(<1..1324)
                     /locus_tag="PKI33_12855"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNM37951.1"
                     /translation="MSKPTETRRYAQEDFSPRTDWLGRLARLNTQFGRFTRDLIGVSL
                     ISFSVMAFFAIWNWTDGFVLTPIALILRTWFGWGSLLILAGIAYLGYSFLQRQQTSLH
                     WGRILALELGALLTLGLLAIPFGNDLFRAESGMDGGRLGWGIVRLLWQMGTIPGTLVL
                     LLLWALSVATGFGLWAKLEAWLIKIAGDEAPVIVSMPEPEVPQEIAVEQPVSKEAAAP
                     KKKAPAIPPEFRTQLKSAEKKPEKSLKPPPRGEDLPPLTVLMGDSTVRTDERTINQTA
                     GLIMKTLSEFGIPATVIGYRVGPSVTQFAVQPGFIKKGNEEEDAQQMKVRVAQIASLE
                     KDLALALSAQRLRIEAPVPGKPYVGIEVPNVNSSIVRMRALLESDAFYKVGAPLTIAL
                     GRDVSGNPLMADLSRMPHLLIAGSTGSGKSVCVTAIAACLAMNNTPEEL"
     gene            complement(1328..>1930)
                     /locus_tag="PKI33_12860"
     CDS             complement(1328..>1930)
                     /locus_tag="PKI33_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNM37952.1"
                     /translation="IGRTLHLKVIECVPEDGRVVFSERAAQTEPGKRAELFHSLHAGQ
                     SVSGTVTNITDFGVFVDLGGVEGLIHISELSWGRVTHPSQFVKLGNAIDVQVLDLAPE
                     RCRVALSLKRMLPNPWTNAANEFPIGSVHNATITSVLSYGVFARLDKYGVEGLVHASE
                     IPHEEGTLLKDFLSDGQAIQVRVLHLDPAHHRLGFSMRLE"
BASE COUNT          531 a          508 c          457 g          434 t
ORIGIN      
        1 gcagttcttc gggcgtgttg ttcatggcaa gacaagccgc aattgcagta acacacaccg
       61 atttaccaga tcccgtagaa cctgcgatca ataagtgcgg catacgagat aaatctgcca
      121 tcaacgggtt gccagagaca tcacgtccca gggcaatggt caacggagcg ccaactttat
      181 agaaagcatc gctttccaac aaggctcgca tgcggacgat agaactgttg acgttcggga
      241 cttcgatacc aacataaggt ttacccggaa ctggcgcctc aatacgtaga cgttgagcgg
      301 atagcgccaa cgcaaggtct ttctccagtg aagcgatttg cgccacacgg accttcatct
      361 gttgggcatc ttcttcttca ttaccttttt tgatgaagcc cggctgaaca gcgaactgcg
      421 tcacagatgg accaactcga tacccgatta ccgttgcggg aataccgaac tctgaaagag
      481 ttttcatgat caaaccggca gtttggttga tcgtgcgttc atcggtgcgg acagttgagt
      541 cgcccatcaa gacagtgaga ggcggtaaat cttcaccgcg tggaggcggc ttgagtgatt
      601 tctctggctt cttttcagca gatttcaact gagtacgaaa ctcaggcgga atggcaggag
      661 ctttcttttt cggagcagca gcttcttttg aaactggctg ttctacagcg atctcttgtg
      721 gaacttctgg ctctggcata gagacgatca caggcgcttc gtcacctgca atttttatga
      781 gccatgcttc aagctttgcc cacagcccaa acccagtagc aacactcaac gcccacaaaa
      841 gcaaaagcac caacgtgccg ggaatggttc ccatttgcca cagcaggcgc acaatacccc
      901 accccaacct tccaccgtcc atgccagatt cggctcggaa gaggtcattg ccaaagggta
      961 tagcaagcaa gcccagggtt aagagagcgc cgagttcaag agcaagaatc cgcccccagt
     1021 gtagagacgt ctgttggcgt tgtaaaaagg aatatccaag ataagcaatc cctgccaaga
     1081 tcaagagact accccaccca aaccaagtgc gcaatatcaa ggcgattggt gtaagcacaa
     1141 agccatcggt ccagttccaa atggcaaaga atgccatgac tgaaaacgag attaggctta
     1201 caccaataag atcgcgagtg aaacgcccaa attgcgtgtt aagacgcgca agccttccga
     1261 gccaatcggt gcgcggagaa aaatcttctt gagcgtaacg ccttgtttca gtgggtttag
     1321 acatccctta ttcaagcctc atgctgaagc caaggcggtg atgtgcaggg tcgagatgca
     1381 atacacgcac ctgaatcgct tgcccgtcag agagaaagtc tttcagcaat gtgccttctt
     1441 catgcggaat ttcagaagca tgaaccaagc cttccacacc atatttatcc agccgtgcaa
     1501 acacgccgta agataaaacg ctggtgatgg ttgcattatg cacagaacca atcgggaatt
     1561 catttgccgc attcgtccaa gggttgggaa gcatacgctt caaactcaag gcaacacggc
     1621 agcgttctgg cgcaaggtct aatacttgca catcaatggc attgccaagt tttacaaatt
     1681 gcgaggggtg cgttacacgc ccccaagaaa gttcagagat atgaatcaaa ccttccacac
     1741 cgccaaggtc tacgaacaca ccaaagtctg taatgttcgt aactgttcca cttacgcttt
     1801 gacctgcatg caatgagtgg aataactcag cgcgttttcc gggttccgtc tgagcagcac
     1861 gttccgaaaa aaccacacgt ccatcttctg gaacacattc aatcactttc aagtggagag
     1921 tacgtccaat
//
LOCUS       DAOREF010000673         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49067_length_1929_cov_4.243330, whole genome shotgun sequence.
ACCESSION   DAOREF010000673 DAOREF010000000
VERSION     DAOREF010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49067_length_1929_cov_4.243330"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKI33_12865"
     CDS             <1..752
                     /locus_tag="PKI33_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNM37953.1"
                     /translation="QHNYFVLSSSASDPQAFQGLVDELVGHRRVDGLIVINPYADERY
                     EFLPKEFPVVFVGARSHDENVCSISLDDQKVAYEATQHLISLGHTRIALVTGPMEEDC
                     SQDRLEGFQRALKEAGISFDSSLMFEGDWSASSGSEALADFVKKGNLPTAVFAQNDRM
                     AMGVMRAARDANLKVPDQLSVIGVDDMPLSSYFDPPLTTMRQDMPLIGQEAIRKLIEI
                     IQNKTVEQTVLKLPAQLVVRQSTVKGGDSTS"
     gene            820..>1929
                     /locus_tag="PKI33_12870"
     CDS             820..>1929
                     /locus_tag="PKI33_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNM37954.1"
                     /translation="MKKNLLVKIMGLLVIASFVLAACGTPAATEAPAQTEAPAVTEAP
                     AATEAPATEASSADVALRWRTRPDNQEEIDVYSKISTELDAQLDGITLTYEPGGSEGA
                     NYQDQLRAEIAAGTAPDVFWIPGTDVADFATRGLILNAADLAANTDGFAVTDFYEGPM
                     YHLTFNPETSTTGADSGALWGLPRDVSTFALYLNMDLINEAGAPDPRELAANGEWNWE
                     SFLEVSQATRALGSDIYGYGASAWWGPYGVWLNASGGGFFNEDRTACALNTPESLAGL
                     DFQRSLYQDYDVAVPYGEDPEPGFRAGKVAMFQNGRWATPGVRTVDFNWDVVELPAGP
                     GGTPGNWLFWGAYVINANTEHPEEAWALVQALTTAE"
BASE COUNT          419 a          509 c          508 g          493 t
ORIGIN      
        1 gccaacataa ttattttgtg ttgtcttcgt cggcttcaga cccgcaggca tttcagggct
       61 tggtagatga gttggtgggg caccgccgag tggatgggtt gatcgtgatc aacccctatg
      121 cggatgagcg ttatgagttt ttaccgaaag aattcccggt ggtattcgta ggtgcgcgtt
      181 cacacgatga gaatgtgtgt tcaatctcgc tcgatgacca aaaagtggca tatgaggcta
      241 cacagcattt gatctcactg ggtcatacgc gcattgcctt ggtcaccgga ccgatggaag
      301 aagattgttc gcaagaccgt ttggaagggt tccagcgcgc attgaaagaa gctggaattt
      361 cgttcgactc gtctttgatg tttgaagggg attggtcggc ttcttctggt agcgaagctc
      421 tggcagattt tgtcaagaag ggcaatttgc cgacagcagt atttgctcag aacgaccgta
      481 tggcaatggg tgtgatgcgt gcggctcggg atgccaacct aaaagtgccg gatcagctct
      541 cggtcattgg cgtagacgat atgcctttat cttcctactt tgacccgcct cttacgacca
      601 tgcggcagga tatgcccctc attgggcaag aagccatccg aaagttgatc gaaattattc
      661 aaaacaagac tgttgagcaa actgtgctga aattacccgc tcaattggtt gtacgtcaat
      721 ctaccgttaa aggaggtgat tcaacctcgt aatacgttta gcgcaaaacc tacttgatcg
      781 tacgatgatt tgttcgtcca attccaagga gaagaataaa tgaaaaagaa tttgctcgtg
      841 aagatcatgg ggctgctcgt tatagcctct ttcgttttag cggcttgtgg cacccctgct
      901 gcaaccgaag cccctgctca aactgaagcg ccagcagtga cggaagcccc agcggcaact
      961 gaagctcccg ccaccgaagc atcgagcgct gatgttgctt tgcgctggcg cacacgccca
     1021 gacaatcagg aagagattga cgtttacagc aagatcagta cagaacttga tgctcagctt
     1081 gatggtatca cattaaccta tgaacccggt ggttctgaag gtgcaaatta tcaggatcaa
     1141 ctccgcgctg aaattgcagc cggcaccgct cctgatgtgt tctggatccc tggaaccgat
     1201 gtggctgatt tcgctacccg tggtttgatc ctcaatgctg ccgacctcgc tgccaacact
     1261 gacggcttcg ctgtcactga cttctacgaa ggtcctatgt accacctgac cttcaaccct
     1321 gaaaccagca ccaccggtgc tgactctggc gctctttggg gtcttcctcg cgatgtgtcg
     1381 accttcgctc tctacctcaa catggatctg atcaacgaag ctggcgctcc cgatcctcgt
     1441 gaattggctg ccaacggcga atggaactgg gaatccttcc tcgaagtttc tcaggctacc
     1501 cgcgctctcg gctctgacat ttacggctac ggcgcttccg cttggtgggg tccttacggc
     1561 gtgtggttga atgcttctgg cggcggtttc ttcaatgaag accgcactgc ttgtgctctc
     1621 aacacccctg agtctcttgc tggtctcgac ttccaacgct ccctctatca ggactacgat
     1681 gtggctgtgc cttacggtga agatcccgaa cccggcttcc gcgctggtaa ggttgccatg
     1741 ttccagaatg gtcgctgggc taccccgggc gtccgcactg tagacttcaa ctgggacgtc
     1801 gttgaattgc ctgctggtcc gggtggcacc ccaggtaact ggctgttctg gggcgcatat
     1861 gtgatcaacg ccaacaccga acatcctgaa gaagcttggg ctctcgttca ggctctcacc
     1921 actgctgaa
//
LOCUS       DAOREF010000674         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49113_length_1928_cov_6.119060, whole genome shotgun sequence.
ACCESSION   DAOREF010000674 DAOREF010000000
VERSION     DAOREF010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49113_length_1928_cov_6.119060"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKI33_12875"
     CDS             complement(<1..374)
                     /locus_tag="PKI33_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37955.1"
                     /translation="MDNLENKSSGGMTSIIVGIVVLLCCLCLLAAGIGGYAYYTFMQS
                     GSTGPIEVFGDPISPDNNPNQPTEPTELVRPSVDSISSETVDTLENAIVPENDPRVLA
                     CRLDGKCGVPEVMATSASPRSVG"
     gene            520..1155
                     /locus_tag="PKI33_12880"
     CDS             520..1155
                     /locus_tag="PKI33_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNM37956.1"
                     /translation="MKKTQPTSPRVQRNREVATSAILDTARAIMREEGVAALSMHELA
                     RRMGMRAPSLYNYFNSKMEIYDALFCLGFNLYNTQLQEALKESKTPHDEIRIAIECYM
                     SFALENPELYQLCFERPVPGFVPSKESLELSFGILNNTYTRIEKFKKQFDTKLTAQQI
                     VDMVIAVAHGLTALHLANEPQLPIGQGRFGSLIPTFVRFFETSMPTKGETK"
     gene            1152..>1928
                     /locus_tag="PKI33_12885"
     CDS             1152..>1928
                     /locus_tag="PKI33_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HNM37957.1"
                     /translation="MTAILSTSNIARAEAIPMTHADEAHRLMTEALERLLSLLESLHA
                     DDWSKPTACTAWNVHDMVAHQAGGYLSGISLREMVRQYSQVPKKGQLPEDAINELQVG
                     ERKNKSHSELIAELKQVGAIAIHNWAYGFNAIKWIAMPHPVGGFMSIRHLMWVIHSRD
                     TWMHRLDICRATNRPFEQTREHDGRIVELVVLDTAKKIKKRLNGHAINLLLTGVAGGT
                     WQIGNGSPVAELEMDALDFNIFVSGRYSFEEGMQRAKISGD"
BASE COUNT          540 a          488 c          425 g          475 t
ORIGIN      
        1 cccaccgaac gcggagatgc cgaagttgcc ataacttcag ggacgccaca tttcccatct
       61 aaacgacacg ccaagacacg cgggtcattc tcagggacaa tggcattttc caatgtatcg
      121 acagtttcag atgagatcga atccaccgac gggcgaacca gttcagtcgg ttccgttggc
      181 tgattcggat tgttatctgg ggaaataggg tcgccaaaaa cttcaattgg tccagtgctg
      241 ccagactgca taaatgtgta ataggcatat ccgccaatcc ctgccgccaa aagacagaga
      301 cagcataaca gcacaacgat tcctacgatg atgcttgtca tgccgccaga tgatttattt
      361 tcaagattat ccatgaatgt tcctctctcc taaaaggttg tccgatgata ccgccatccg
      421 ctaaaaaacg gattaggact ttaggggtaa tggtgagcca atctcttgac aaaggcaatt
      481 tttctaatat aattagaaaa aactaacaat attagaaaaa tgaagaaaac acagcccacc
      541 tccccccgtg ttcaacggaa ccgtgaagtc gccacctctg ctattttgga tacggcccgc
      601 gctatcatgc gtgaagaagg ggttgctgcc ctctccatgc atgagttggc gcgtcgtatg
      661 ggcatgcgcg ctccgtcact ctacaactac ttcaacagca aaatggaaat ctatgatgcc
      721 ctcttctgtt tggggtttaa cctgtataac actcaacttc aagaagcgct caaagaatca
      781 aagactccac atgacgaaat tcggattgcc atcgaatgtt acatgtcttt cgccttagaa
      841 aaccctgagc tgtaccaact atgttttgag cgcccggttc ccggttttgt gccctccaaa
      901 gaaagcctcg aactcagttt tggcattctt aacaacacct atacacgcat agaaaaattc
      961 aagaaacaat tcgatacaaa actcaccgct caacagatcg tggatatggt cattgcagtc
     1021 gctcacggtc tcacagcttt gcatcttgcc aacgaaccgc aacttcctat tgggcaagga
     1081 cgcttcggtt ctcttatccc tactttcgta cgtttctttg aaacgtctat gcccaccaaa
     1141 ggagaaacca aatgaccgcc atcctttcga catcaaacat cgcccgcgct gaagccatcc
     1201 ccatgaccca tgcggatgaa gcccatcggc tcatgaccga agccttggag cggcttttga
     1261 gcctgctcga gtctctccac gctgacgatt ggtctaaacc tacagcctgc acagcatgga
     1321 atgttcacga catggtagca caccaggcag gtggatactt aagtggcata agtttacgag
     1381 agatggttag gcaatattcc caagttccca agaaaggaca attgcccgaa gatgccatta
     1441 atgagttaca ggtgggagaa cgcaagaata aatctcattc tgaattaatc gctgaactaa
     1501 aacaagtcgg tgctattgcc attcataact gggcatatgg tttcaatgct atcaaatgga
     1561 tcgctatgcc gcacccagtt ggcggtttca tgtcgattcg tcacctaatg tgggtcattc
     1621 acagccgcga tacatggatg catcgtctgg atatctgccg cgctaccaac cgtccttttg
     1681 agcaaacgcg tgaacatgat gggcgtattg tggagcttgt cgttttagat acagcgaaaa
     1741 aaataaaaaa acgtctcaat ggtcatgcta ttaatttact gttgacaggc gtcgctggcg
     1801 gaacatggca aattggaaat ggttctcccg ttgcagagct cgaaatggac gcactggatt
     1861 tcaacatatt cgtctcaggt cggtactcgt ttgaagaagg catgcaacgc gcgaaaatct
     1921 caggcgac
//
LOCUS       DAOREF010000675         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49115_length_1928_cov_5.725040, whole genome shotgun sequence.
ACCESSION   DAOREF010000675 DAOREF010000000
VERSION     DAOREF010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49115_length_1928_cov_5.725040"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKI33_12890"
     CDS             <1..967
                     /locus_tag="PKI33_12890"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNM37958.1"
                     /translation="VTEKNAGRIGIIISSAIGGLYSLQEATLTNYKEGPRKVSPFMIP
                     MLMANGGAGMAAIEFGIKGPCFSVASACASGADGIGTALMMLRTGMIDAALAGASEAT
                     ITSTGVAAFDRVGAMSRRNDNYSMTPQPFDKNRDGLVMGEGAAVLVLERESDAKARGA
                     NILAELAGYGATADAFHVTAPHEHGAGGAAAIRMALDSAKANIDEVGYINAHGTGTSL
                     NDQAETRAVKAAFGDLAYKTPISSTKSMTGHMMGATGALEVIFCVNAIRDGILPPTIH
                     YETPDPECDLDYIPNKAREAKISLAISNAFGFGGHNAVLAIRKYS"
     gene            1053..1400
                     /gene="rplS"
                     /locus_tag="PKI33_12895"
     CDS             1053..1400
                     /gene="rplS"
                     /locus_tag="PKI33_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNM37959.1"
                     /translation="MADLIKAVEAKDNEKIPQLAAGDTVSVHVKIKEGDRERIQEFKG
                     LVIRLRNSGNNASVTVRRMASNGVGVERTFLLRSPRVDKIVVERHGKVRRAQLYYMRE
                     RTGKSARIKEKFS"
     gene            1511..>1928
                     /locus_tag="PKI33_12900"
     CDS             1511..>1928
                     /locus_tag="PKI33_12900"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNM37960.1"
                     /translation="MTKPLIGITTFNRNNDYGKPMSGVQHTYIRAVVQGGGIPILIPA
                     ILDEEDRNDLYARVQGVLFTGGGDVSIKYFNGVDHPKIAEVDESRDITEISLLKKTVE
                     DGKPFLAVCRGVQVMNVALGGTLYTHIPDQFPNALHH"
BASE COUNT          467 a          436 c          527 g          498 t
ORIGIN      
        1 ggtaaccgag aaaaatgccg ggcgtattgg cattattatt tcttctgcga tcggtggttt
       61 gtattcattg caggaagcca ctctcacaaa ttacaaagaa ggtccgcgta aagtaagccc
      121 gttcatgatt cccatgttga tggcgaatgg tggggcaggc atggcggcaa ttgaatttgg
      181 aattaaggga ccatgttttt ctgtggcttc ggcttgtgct tctggtgcag acggaattgg
      241 tactgcgcta atgatgctcc gcacgggcat gattgatgct gcgctggcag gagcttctga
      301 agcgacgatc acatctacgg gtgtggctgc atttgaccgc gtaggcgcta tgtctcgccg
      361 aaatgacaat tattcaatga ccccccaacc gtttgataag aatcgggacg gtttggtgat
      421 gggcgaaggg gcggctgttc tcgtgcttga acgtgagtcg gatgcgaaag cacgcggtgc
      481 aaatattctg gctgaattag ccgggtatgg tgccactgct gatgctttcc atgtgacggc
      541 tccgcatgaa catggggcgg gcggtgcggc agcaattcgt atggcattag actctgccaa
      601 ggctaacatt gatgaagttg gttacattaa cgctcatggc accggaactt cgttgaacga
      661 ccaagccgaa acccgcgctg ttaaggcggc atttggtgac ttggcgtata aaacgccaat
      721 ttcctcaacc aagtctatga cggggcatat gatgggtgct acgggtgctt tggaagtgat
      781 tttctgtgtg aatgccattc gagacgggat tttgccgcca accatccact acgaaacccc
      841 cgaccccgaa tgtgacttgg actatattcc gaacaaggct cgtgaggcga agatttcgct
      901 ggcaatcagc aatgctttcg gttttggcgg gcacaatgca gtgctggcaa ttcgaaaata
      961 ttcctgattt ttgcttgtga tttgagatta tccgtgctat aatccgccct cgcgtatatt
     1021 ggaaacctaa tcataaagta aggaagaacg aaatggcaga cttgattaaa gccgtagagg
     1081 caaaagataa cgagaagatc ccccagcttg ctgctggcga cactgtgagc gttcacgtga
     1141 aaattaagga aggtgatcgt gagcgtattc aggaattcaa aggcttggta attcgcctgc
     1201 gaaacagcgg caacaacgct tcggtgactg tgcgccgcat ggcgtcgaat ggcgttggcg
     1261 tcgaacgcac cttcctgctc cgctccccgc gcgtggacaa aatcgtggtg gaacgccacg
     1321 gtaaggtccg ccgtgcccag ctctactaca tgcgcgaacg caccggcaag agcgcccgca
     1381 tcaaggaaaa gttctcctag tttttcctta ttgaatcccg atggcaaccc catcgggatt
     1441 tttttgttta taaccgcttg tttggctgtg gtatgcttat attcgtaaat ttcttgtttt
     1501 ggaggaacct gtgaccaaac cattaatcgg catcacaact ttcaatcgca ataatgatta
     1561 cggcaagcct atgtctggcg tgcaacacac ctatattcgt gcggtggttc aaggtggggg
     1621 cataccgatc ttgatcccgg cgattttgga tgaagaagac cgaaacgatt tgtatgctcg
     1681 ggtgcaaggt gttttattta caggcggcgg agatgtgtct attaaatatt tcaacggcgt
     1741 agaccacccg aaaattgcag aggtcgatga gtcgcgcgat atcactgaga tttcattgct
     1801 gaaaaaaaca gtggaagatg gaaagccttt tctcgcagtt tgccgcgggg ttcaagtgat
     1861 gaatgtcgct ttaggcggca ctctgtatac gcatatccca gatcaattcc ctaatgcgtt
     1921 gcatcacg
//
LOCUS       DAOREF010000676         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49173_length_1927_cov_2.697650, whole genome shotgun sequence.
ACCESSION   DAOREF010000676 DAOREF010000000
VERSION     DAOREF010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49173_length_1927_cov_2.697650"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKI33_12905"
     CDS             <1..239
                     /locus_tag="PKI33_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37961.1"
                     /translation="EYQPPLSFAFTGKDETGIFKHQFRLSPEPNGTRMERRVSFELSF
                     RQWLMYCLLLFPVRLPAARKALRRLKEYVEAGQP"
     gene            370..1230
                     /locus_tag="PKI33_12910"
     CDS             370..1230
                     /locus_tag="PKI33_12910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNM37962.1"
                     /translation="MLRRLFAPFNKFFLSIGSRRVFVLASLLLLVLGWCDYATGFEIA
                     FSFFYLIPISIATWYIGIRSGYLMTVVGLLLWVVSNRLAGEMYSSEWIRFFNTAVRLV
                     VFMMIANLLHELKIALQAEHKIAHTDHLTGAYNSRELDEQLTFEIQRASRQHYPISLA
                     YIDLDNFKRVNDTYGHSAGDLQLKRIAQTLSDTIRKTDILARLGGDEFVLLLPNVDSE
                     DAKLVFQKVERAVLQELADLHSPITLSAGVITFKKIPASADEMLRQADALMYEAKNSG
                     KAKALYFVVE"
     gene            complement(1254..1754)
                     /locus_tag="PKI33_12915"
     CDS             complement(1254..1754)
                     /locus_tag="PKI33_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37963.1"
                     /translation="MFQLWLASFIHNDRSEIILKRIEAMNITPSEPPVTPSGKSRLGL
                     FSTLLGASLYLIPIASFLILVVVSSILGLIDGETPASWFYEKDKTLGGLVFVIGLFFS
                     VPVVGILILFVSTTGLVLGVLSLRRKSERKGLAIAGILLNVLPFLITACLALLSLLNS
                     LMSINL"
BASE COUNT          493 a          466 c          469 g          499 t
ORIGIN      
        1 acgaatatca gccgcccttg tctttcgcct ttaccggcaa ggacgagacc gggatcttta
       61 agcaccaatt tcggttaagc cctgagccga acggcacccg catggaacga cgcgtatcct
      121 tcgagctatc gttccggcag tggctgatgt attgcctgct attgttcccc gtgcgtttgc
      181 ccgccgcccg aaaggcattg cggcggttga aagagtatgt agaagccggt cagccctaag
      241 cgaccgcctt tcagggagag gtttgccccc ataagcggac aaacctctcc cttttcatac
      301 ctcgttgcgg cggctctttc gattgtgaat gttctatcta cgcggctttc atgtacaatg
      361 aaatcagata tgcttcgccg cctcttcgcc ccgttcaaca aattcttttt atccatcgga
      421 agccgccgcg tctttgtttt ggcaagtttg ttgctgttgg tgctaggctg gtgcgactat
      481 gcgaccggct ttgaaattgc cttttctttc ttttatctga tccccatctc gatcgccacc
      541 tggtatatcg gtattcgcag cggctatttg atgaccgtgg tgggactgtt gctttgggtg
      601 gtttctaacc ggctggcggg ggaaatgtac agcagcgaat ggatccggtt ctttaacaca
      661 gcggtgcgtt tggtggtctt tatgatgatc gccaatttgt tgcatgagtt aaaaatcgcg
      721 cttcaggcag aacataaaat tgcccatacc gaccatttga caggggcgta taacagccgt
      781 gaactggatg agcagctaac ttttgaaatc caacgggcga gccggcaaca ctatccgatt
      841 agtttggcgt acattgacct cgacaatttc aagcgggtca atgacaccta cgggcacagc
      901 gccggggatt tgcaattaaa gcgcatcgca cagaccttga gtgacaccat ccgcaaaacg
      961 gatattcttg cccggttggg cggagatgaa tttgtgttgt tactgccaaa cgtggacagt
     1021 gaagatgcga agttggtctt tcaaaaggtg gaacgggcgg ttctgcaaga attagcggac
     1081 ctgcattccc ccattacgct cagcgccggg gtgattacct tcaagaagat tcctgccagc
     1141 gccgatgaaa tgctccgtca ggcagatgcg ttgatgtatg aagccaagaa ttcaggcaaa
     1201 gccaaggcgc tgtattttgt tgtagagtag gggtaagcaa atcccaacgg atattatagg
     1261 ttgatactca ttagggaatt gagcagggat agtagcgcca gacaggcagt tatcagaaaa
     1321 gggagtacat tcagaaggat acccgcgatc gccaatcctt ttctttcgga tttacgcctg
     1381 agactcaaca ctccgagcac cagccccgtt gtgctcacaa acagaataag tatcccaacg
     1441 accggaacgg aaaaaaaaag tccaataaca aaaaccaagc ctcctaaggt cttatctttc
     1501 tcataaaacc aacttgccgg agtttctcca tcgatcaacc ccaaaatact ggataccaca
     1561 accaagataa ggaaactcgc tatgggaatc agataaaggc ttgccccgag gagggttgaa
     1621 aacaagccga gcctgctttt gccagatggg gtgacgggtg gctctgaggg agttatattc
     1681 atcgcttcaa tcctttttaa aataatctca cttctatcat tatgaataaa cgaagccagc
     1741 cagagctgaa acattcagga gaacaggcta tggcatgatt gattttatat cttaatttcg
     1801 ttttattgcg ttagatttta gggaatattc taagacccat cctttcaaaa cggactttgc
     1861 tctacaagtg aataagctgc ttccatcccc aaagtgacag tcaccttaaa ggtgactgtc
     1921 acttgat
//
LOCUS       DAOREF010000677         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49598_length_1918_cov_5.336554, whole genome shotgun sequence.
ACCESSION   DAOREF010000677 DAOREF010000000
VERSION     DAOREF010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49598_length_1918_cov_5.336554"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1562)
                     /gene="ltrA"
                     /locus_tag="PKI33_12920"
     CDS             complement(81..1562)
                     /gene="ltrA"
                     /locus_tag="PKI33_12920"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNM37964.1"
                     /translation="MTNDWRESDKLVVPKKLVNKVGPNSTAAEPMEGRSLTKGNSPGQ
                     NIHQTQGWESVQSALLAIRKTAKADKKVQFTALLHHVYNVDALRQAFASLKHNASAGV
                     DGRTWQDYEENLTDNLMELSARLKRGAYHAKPVRRVFIPKQDGSKRPLGVTALEDKIV
                     QKAVVEVLNAIYEADFQGFSYGFRPGRSQHSALDALYVGFMKRKVGWVLDADIRDFFS
                     AIDHEWLVKFIEHRIADKRIVRLIQKWLKAGVLENGERTPSEDGLPQGGCVSPVLANI
                     YLHYVFDLWAHQWRNRNAYSDVIIVRYCDDFIVGFHHKRDAERFLDELKARLARFKLE
                     LHPDKTRLIEFGRYAIESRKRRGLGRPETFNFLGFQHICGVRRDGIFTVLRHTVKKKM
                     NAKLREVKTELRWRMHKPISETGAWLKAVVRGHFNYYGVPTNGKQLGLFRNSIIRLWR
                     HALKRRSQRNRITWEKMAQIAHTWLPLPRITQPYPLERLIVRT"
BASE COUNT          381 a          567 c          429 g          541 t
ORIGIN      
        1 agtcgcggta gggatcagag ttacccccga ccccccgcac cgatccgcac gagcgctcgt
       61 tagcgcatgc ggctcctacc ttaggtcctg acgatgaggc gctcaagtgg atagggttgt
      121 gttatacgag gcaacgggag ccatgtatgc gctatttggg ccattttctc ccacgtaatt
      181 cgatttctct ggcttcgtct tttcaaggcg tgtcgccaga gtcttattat cgagttgcga
      241 aaaagaccta gttgcttgcc gttggtcggc actccataat aattgaaatg tcctctcaca
      301 acggctttga gccatgcccc tgtctcagaa atgggtttgt gcatgcgcca cctcagttcc
      361 gttttcactt ctctgagttt tgcattcatc ttctttttca cggtgtgcct aagcactgta
      421 aagataccgt ctctccgaac cccgcagatg tgttggaagc ccaagaaatt gaaggtctct
      481 ggtctaccta gaccgcgcct ctttcgagat tctatggcat atcgcccgaa ctctattagg
      541 cgcgttttgt ccgggtgaag ttctagtttg aacctagcca gtctggcttt cagttcatcc
      601 aagaatcgct cagcatcccg cttgtggtga aacccgacaa taaagtcatc gcaatatctc
      661 acgattatta cgtcactgta tgcgtttctg tttcgccact gatgtgccca caaatcaaac
      721 acgtaatgca gatagatatt ggctaggacg gggcttacgc acccgccttg cggcagtcca
      781 tcctcacttg gtgtcctttc cccattctca agcacaccgg ctttcaacca tttctggatg
      841 agtcgcacaa tgcgcttgtc cgcgatgcga tgctcaatga atttaaccag ccactcgtga
      901 tctatggcag aaaagaaatc ccgaatgtct gcatcaagca cccagcccac ctttctcttc
      961 atgaagccga cataaagggc atccaacgca ctgtgttggc ttcgccccgg tcggaatcca
     1021 tacgagaatc cttgaaagtc cgcttcgtat atggcattca acacttctac aactgccttt
     1081 tggactatct tgtcctccaa cgcagtcacc ccaagtggtc gtttgctacc atcttgtttc
     1141 ggtatgaaca cccgcctaac gggctttgca tggtacgctc cccgcttcaa tcttgccgag
     1201 agctccatca gattatctgt caagttctct tcgtaatctt gccatgtcct tccatcaacg
     1261 cctgcgcttg cattgtgctt taggctcgca aaagcctggc gcaaagcatc gacgttgtag
     1321 acatggtgca ggagtgctgt gaactgcacc ttcttatccg ctttcgcggt cttccgtatc
     1381 gccagcagcg cactttgcac actttcccag ccctgagtct ggtgtatgtt ttgccctggc
     1441 gaattccctt tggtcaagct tcttccctcc atcggctccg cagccgtcga atttggtccg
     1501 actttgttca ccaacttctt tggtactaca agcttgtccg actccctcca atcgttcgtc
     1561 atcggattgc tcctctggat tcccgatgcg gaccggttgc cttccggtca attgcagggt
     1621 ctcccgtttc ccgctcaaag agtttccatg cgtgttggtg gtctctgact ccgcggggtc
     1681 taactgcatc ttgcgatagc gttacagcta gtgttgcctt ccgtataata tgacaacgtc
     1741 ggcaccccgg actaaacttt cgaagctcta tacacccccc gcatgtgccc ctgtcaacgc
     1801 ttcgtgtgta cctctcgata ctggtcacgc atgactcggg gtcgacttgg ttcgctagac
     1861 cttagtcgta gggctctctc atcccctcct ccttgccggt tttaaacggc gcaccata
//
LOCUS       DAOREF010000678         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49603_length_1918_cov_3.947933, whole genome shotgun sequence.
ACCESSION   DAOREF010000678 DAOREF010000000
VERSION     DAOREF010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49603_length_1918_cov_3.947933"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKI33_12925"
     CDS             <1..463
                     /locus_tag="PKI33_12925"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNM37965.1"
                     /translation="IHRDLHKLANAGQIIKKHGGAALPNSGVKALEEPSSCAMCGKPA
                     SQRTIFIVKFDNGEELRACCAHCGLMMQSREKNVWQSLTVDFLYGHMISAGQAYYLVG
                     SELNICCVPSILAFGSKADAEKFQKGFGGQLLDMEDTIHHMHGMMHMHSDM"
     gene            496..1011
                     /locus_tag="PKI33_12930"
     CDS             496..1011
                     /locus_tag="PKI33_12930"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNM37966.1"
                     /translation="MKKIFAFLFASALLLSACGGGMAGGEEHTDGEGVEAHDYWVRAA
                     MAGGNSAAYMLLHNHGAEDDALIAVSSDLADAVEMHLSQMKADGTMEMIQQEKIDLPA
                     DGEVELKPGSYHIMFIGLKQDLVAGNEITLTLTFEKAGDITLTVPVKDSADMGGESMN
                     MGGMDMATPTP"
     gene            1261..1746
                     /gene="nrfH"
                     /locus_tag="PKI33_12935"
     CDS             1261..1746
                     /gene="nrfH"
                     /locus_tag="PKI33_12935"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNM37967.1"
                     /translation="MSKLPIIAIGIAVLALGYFALVSDAAAYGGNAPETCANCHAMDS
                     QYENWAHGGHAKVAKCTDCHLPHDNFVSYYMEKGRQGAKDTYAFVTGDIPVAIRAEEK
                     TKEIVQENCIHCHEDTVDTMLAGAQPFDRYCWECHRSVAHGERGATGAPYQDSVIYPT
                     K"
BASE COUNT          498 a          383 c          547 g          490 t
ORIGIN      
        1 catccaccgc gatttgcata agttagcaaa tgcgggtcag ataataaaga aacatggcgg
       61 tgctgcctta ccgaactcgg gcgtaaaagc attagaagaa ccatcttcat gtgccatgtg
      121 cgggaaaccc gcttctcaaa ggacgatctt catcgtcaaa ttcgataacg gggaagaact
      181 gcgtgcttgc tgtgctcatt gtggtttgat gatgcaaagc cgtgaaaaga acgtttggca
      241 atctctgaca gtcgattttt tatatgggca tatgatcagt gccgggcagg cttattacct
      301 ggttgggagt gagctaaata tttgctgtgt accaagtatt cttgcattcg gctcaaaggc
      361 agatgcagaa aagtttcaaa aggggtttgg tgggcaattg ctcgatatgg aagataccat
      421 tcaccatatg catggaatga tgcacatgca ttcagacatg tgattcagaa aactaaattt
      481 gtgaggaata agagaatgaa aaaaattttt gcttttcttt ttgccagtgc gcttctgtta
      541 agtgcatgcg ggggtggtat ggcaggtggg gaagaacaca ccgatgggga aggtgtggaa
      601 gctcatgatt attgggtccg cgcagcgatg gcaggtggca atagcgctgc ttatatgctc
      661 cttcataacc atggggcaga agatgatgcc ctcattgctg tcagttctga tttggcagat
      721 gcggttgaaa tgcacctgag ccaaatgaaa gccgacggca ccatggaaat gatccagcaa
      781 gaaaagattg atttgcctgc cgatggtgaa gtggaattga agccgggtag ctatcacatc
      841 atgttcattg gtctgaagca agaccttgtg gctggcaacg aaattacact gaccctgacc
      901 tttgaaaaag cgggtgatat caccttgacc gtccctgtga aggactctgc agatatgggt
      961 ggagaaagca tgaacatggg cggaatggat atggcaaccc cgaccccgta atttttttga
     1021 atacgcgtct tcgccagacg cattctcatt tgctctgctg aacaccttca gcagggcgtt
     1081 tttatttgtg ggaatgggaa aggtttgcag cgctgaatcc attgtccttt agcgaggagg
     1141 tgaatgtcat gaagtatgat gacaatcctc ctgcttgggg tggagtgtaa ctaaagtcac
     1201 aggaaattcc tttatcctta gctatagtag ggtcaggtaa atttaatgcg aggttaattt
     1261 atgtctaaac ttcctatcat tgctattggg atcgcggtgc tggcgctggg atattttgcg
     1321 ctggtgtcgg atgccgctgc gtacggcggg aacgcgccag agacgtgtgc gaactgccat
     1381 gcaatggact cgcagtatga aaactgggcg catggggggc atgccaaggt ggcaaagtgt
     1441 accgactgcc atttgcctca cgataatttc gtttcgtatt acatggaaaa aggacgccag
     1501 ggcgcgaagg atacctatgc ctttgtgacg ggggatattc cggttgccat tcgtgcggaa
     1561 gagaagacga aagagatcgt gcaggagaat tgcatccatt gccatgaaga tactgtagat
     1621 accatgctgg cgggcgcaca gccgttcgac cggtattgct gggagtgtca ccgcagtgtt
     1681 gcgcatgggg agcgtggggc gacaggtgcg ccgtatcaag attctgtaat ttatccgacg
     1741 aagtaaggag aatacaaaat gaaaaactat acaactttaa tacttgccgg gttggtgatc
     1801 ttgcttgccg cggtattgat tggcgtaatg gtctacatcc aaaatcagcc tgcggaagtg
     1861 cgcgccgtgc agccgttggt ggagattaag gcaatggagc cggactcttc caaatggg
//
LOCUS       DAOREF010000679         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49948_length_1911_cov_4.962823, whole genome shotgun sequence.
ACCESSION   DAOREF010000679 DAOREF010000000
VERSION     DAOREF010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49948_length_1911_cov_4.962823"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKI33_12940"
     CDS             <1..1093
                     /locus_tag="PKI33_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNM37968.1"
                     /translation="GVCAFALVSSGMLQSLLEPVVTPTIAATSTISPATEVTETSEAI
                     TATVEVPPTENVPTLPLMGGADKIAFIKNNDIWVMNVDGSELKQHTNDGLPKFNLQWM
                     PDGKNLLYMTGKTVKTVNIDTEVEEVLMNFVSAEYFEGFSVSPNGKQAAISINRELFI
                     VPFEIETLRAATRKSALLEMKGCFYNDLGIKELQWSDDSTKLAVEFIANSNGSFADAI
                     RVYDVQDCEKISNSKIDEFPAGRFQFSGDLSSFDWDGDLLFFLTSNTRNGGFGDLALY
                     NIFTHKAEKAAPYESNCCYRDAAFSPDGTQVIFAFQDIRLAGNSPINLYIIPADSIST
                     PRKLEPILPMDFFTKRDDAPQPVMRAAQP"
     gene            complement(1090..>1911)
                     /locus_tag="PKI33_12945"
     CDS             complement(1090..>1911)
                     /locus_tag="PKI33_12945"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNM37969.1"
                     /translation="YDKDGIAIRGLAALGFGHVEVGTVTPKPQPGNPSPRVFRLVEDD
                     AVINRMGFPSRGMEYLEQQLSGGANWNTVLGVNLGKNKETPNEEAVSDYLALLKKFAA
                     YANYLTINVSSPNTVGLRSLQGREALEGLLTQLHQQRLAEQEKCKKRLPILVKLAPDL
                     SDAELSDALEAILTTNMDGVIVTNTTLSREGLKSSYREETGGLSGSPLTVKSEAVLRQ
                     TLKFVNGKIPVVSAGGVMNSEDAKRRLDMGAALVQVYTGLVYRGPGLVKEIVRAL"
BASE COUNT          497 a          531 c          413 g          470 t
ORIGIN      
        1 cggcgtatgt gcttttgccc tcgttagcag cggcatgctt cagtctttgt tagagccggt
       61 tgttactccg accattgctg ccacatctac aatctcacct gctactgaag tgactgaaac
      121 atccgaagcc atcacagcaa ctgttgaagt gcccccaacc gaaaatgtgc cgaccctgcc
      181 tttgatgggc ggagccgata agattgcatt catcaagaac aatgatattt gggtgatgaa
      241 tgtagatggc agtgaattaa aacaacatac caacgatggc ttgccaaagt ttaatttgca
      301 atggatgcca gatggcaaga accttcttta tatgaccggt aaaacggtga agacggtgaa
      361 cattgataca gaagtagaag aagtattgat gaactttgtt tcagcagaat acttcgaagg
      421 tttcagtgtt tcgcccaatg gcaaacaagc agccattagc atcaaccgtg aactattcat
      481 tgtgccattc gagattgaaa cactgcgtgc cgcaactcgc aaaagcgctt tacttgaaat
      541 gaaaggttgt ttttacaacg acctcggcat caaagaactt caatggtctg atgacagcac
      601 caaactagca gttgagttca ttgcgaattc caacggcagc tttgccgatg ccattcgtgt
      661 gtatgatgtg caagattgcg aaaagattag caactccaag atcgatgaat tccccgctgg
      721 gcgtttccaa ttctcaggcg acctctccag tttcgattgg gatggtgacc tgctcttctt
      781 cctcaccagc aacacacgca atggcgggtt cggagatctg gcgttataca acatcttcac
      841 ccacaaagcc gaaaaagccg cgccgtatga aagcaattgt tgctatcgcg atgctgcctt
      901 cagccccgat ggcacccaag tgatctttgc ctttcaagat attcgtctgg cgggcaatag
      961 tccaatcaat ctctatatca ttcccgctga ctcaatttcc acgccgcgca aactcgagcc
     1021 catccttcca atggatttct tcaccaaacg agacgatgcc ccacaacctg tcatgcgcgc
     1081 tgcacaaccc taaagagcgc gaacgatctc tttcaccaaa ccgggtccgc gatagaccaa
     1141 ccccgtataa acctgcacca gcgccgcccc catatcgagg cggcgttttg catcctcaga
     1201 attcataact ccgcccgcac tcaccaccgg gattttccca ttcacaaact tcaaggtctg
     1261 acgaagaacc gcctcgctct taaccgtaag cggactgcca ctcaacccgc ctgtttcttc
     1321 ccgataactt gactttaatc cctcacgact caatgtggtg ttcgtgacga tcacaccatc
     1381 catgttcgtg gtgaggatag cttccaaggc atcgctcaat tcagcatcag aaaggtctgg
     1441 cgccaacttc acaagaatgg gcaaacgctt cttacacttt tcttgttccg ccaaacgctg
     1501 ttgatgtaat tgcgtgagca acccctccaa ggcttcacgt ccctgcaagc tgcgaagtcc
     1561 caccgtattt ggcgagctga cgttaatcgt cagataattg gcatatgcgg caaacttttt
     1621 caacaatgcc aaataatcag agaccgcttc ttcgttggga gtctctttgt tcttgccaag
     1681 gttaactcca agaaccgtat tccaatttgc gccaccagaa agctgttgtt ccaaatattc
     1741 catcccacgt gaagggaagc ccatgcggtt gataaccgca tcatcttcca ccaaacgaaa
     1801 cacccgcggg ctggggtttc ccggttgcgg cttgggggtg accgtgccca cctctacgtg
     1861 accaaaccct agagcagcaa gtccgcgaat ggcgatgcca tctttatcgt a
//
LOCUS       DAOREF010000680         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_49949_length_1911_cov_4.200431, whole genome shotgun sequence.
ACCESSION   DAOREF010000680 DAOREF010000000
VERSION     DAOREF010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49949_length_1911_cov_4.200431"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKI33_12950"
     CDS             <1..189
                     /locus_tag="PKI33_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNM37970.1"
                     /translation="MQTYENLMAGSRNGSVITPGNADDSELVRLIVRGKMPNRGQKLS
                     DEEIQLISDWINQGALNN"
     gene            282..1040
                     /locus_tag="PKI33_12955"
     CDS             282..1040
                     /locus_tag="PKI33_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HNM37971.1"
                     /translation="MTERPYQPKPAIQQKGTLFLTNFKLPDVSFWQDDPTTTQPINFN
                     QMATMTRGVIIRAGQGTYADRVFSTSWKNARNAGLIRGSYWFYDSRVNPKRQAEKWVE
                     ILGNDPGEMEMWCDYEDRYGGPFGGWRNWFDFMERIKTLLPEKKLGVYTGYYYFQEFA
                     AGVNYFAQYPLWIAWYNPTMPLIPPIWQDWTYWQFTDDGDGTLFGVESREIDLNYFNG
                     SEAEFLARYPAPQTTEATLIAKFGNTLVEYRRVS"
     gene            1037..1408
                     /locus_tag="PKI33_12960"
     CDS             1037..1408
                     /locus_tag="PKI33_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNM37972.1"
                     /translation="MTKYSMTPLNSGTRIRTDHSTFGSVITSVARGQLVVGDELWEAT
                     ADGTEVKKGDKWLHVTSVDGVNLAEAGWMAYIHKGVVICNNFTEIEEPNPNPTPVFPE
                     SFTLTDPSGAKAEYKFVRIIE"
     gene            1492..1842
                     /locus_tag="PKI33_12965"
     CDS             1492..1842
                     /locus_tag="PKI33_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNM37973.1"
                     /translation="MRPKQQVFAMKFSDVYPLYIQKAERKNRTKAEVDQIICWLTGYT
                     QAKLQQQIKQEHDFETFFAQAPAIHPNTSLIKGVVCGVRVEEIEDPLMQKIRFLDKLI
                     DELAKGKAMEKILR"
BASE COUNT          570 a          450 c          434 g          457 t
ORIGIN      
        1 atgcagacct atgaaaattt aatggctggg tctcgcaatg gttctgtcat cacgcccggc
       61 aatgctgatg atagcgaact cgttagattg atcgtgcgtg gcaaaatgcc gaatcgcgga
      121 caaaagctgt ctgatgaaga aatccagctc atcagtgatt ggatcaacca aggcgcttta
      181 aacaactaat aaaagccaga aagaccaatc accacagaga taactctgtg gtgatttctt
      241 ttaacattag ccgaatagtt gttatataat tgccaaaagc catgacagag cgcccctacc
      301 aacccaaacc cgctattcag caaaaaggga ctttgttcct gaccaatttc aagctacctg
      361 atgtcagctt ctggcaggat gaccctacca ctacccaacc catcaacttc aatcaaatgg
      421 caaccatgac ccgcggagtc atcatccgcg ccgggcaagg gacatatgca gaccgcgttt
      481 ttagcacttc atggaaaaat gctagaaatg ctggtctgat tcgtggttcc tattggtttt
      541 acgatagccg cgtaaaccca aaacgccaag ccgagaagtg ggtggaaata ttagggaatg
      601 accccggcga aatggaaatg tggtgcgact atgaagaccg gtatggtggt ccattcggag
      661 gctggcgtaa ctggttcgac ttcatggaac gcatcaaaac cttattacca gaaaagaagc
      721 taggggttta tacaggctac tattattttc aagaatttgc agctggtgta aattacttcg
      781 ctcaatatcc attatggatc gcatggtaca accccaccat gccgctcatc ccacccatct
      841 ggcaagattg gacctactgg cagtttacag atgacggcga tggcactctc tttggcgttg
      901 agagccggga gatcgatcta aattacttta acggcagtga agccgagttt cttgctcgat
      961 accctgcccc acaaaccacc gaagccaccc tgatcgccaa atttggcaat acccttgttg
     1021 aatataggag agtctcatga ccaaatatag catgacccca ctaaacagcg gaactcgcat
     1081 ccgtaccgac cacagcacat ttgggtctgt gattacctct gtcgcacgcg gacaattggt
     1141 agttggcgat gaactctggg aagccactgc cgacggaacc gaagtgaaaa aaggcgataa
     1201 atggctgcat gtcacatcag tagatggagt caacctcgct gaagcgggtt ggatggcata
     1261 catccataaa ggtgtggtca tttgtaataa ctttactgaa atagaagaac cgaatccaaa
     1321 tcccacacct gttttccccg agtcttttac acttacagac ccaagtggcg ccaaagcaga
     1381 gtataagttt gtaagaatta ttgagtaata gttagaggaa tgagcgttga cagcttgatg
     1441 ctgtcagcgc tcccgtattg ggggattgta gatcgaaaca ccggagaagt tatgagaccc
     1501 aaacaacaag tttttgccat gaagttttca gatgtatatc cgctttatat tcaaaaagca
     1561 gagcgtaaga accgcacaaa agccgaagta gatcagatta tttgctggtt aactggctat
     1621 acccaagcaa agctgcaaca acaaattaag caagaacatg attttgaaac ctttttcgct
     1681 caagcgccag ctattcatcc aaacacctct cttatcaaag gtgtcgtgtg cggcgtgcgt
     1741 gtggaagaaa tcgaagatcc attaatgcaa aaaatccgct ttttagataa attaatcgac
     1801 gaactcgcaa aggggaaagc gatggaaaag atcttacgat agcatcattt ttctgtttgg
     1861 acggcttcgc cgtccccgcc ccagcgcagg taacgcaaac cgttaggcgc t
//
LOCUS       DAOREF010000681         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_50495_length_1900_cov_3.411382, whole genome shotgun sequence.
ACCESSION   DAOREF010000681 DAOREF010000000
VERSION     DAOREF010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50495_length_1900_cov_3.411382"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PKI33_12970"
     CDS             <1..1556
                     /locus_tag="PKI33_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNM37974.1"
                     /translation="RVTVAQRQKRIGDILLLTEEISNPLIKEEAIGEAGNALANMGRN
                     ELALVQYRKGLEVNSGNLDFRRKEAFHLNRLGRVDEAIVKIESLLTDFPNDSEAISYL
                     GRIYKEMWADSWKQIPDKNKRLKAAFDAYHWLIKAIDTYLKGYRIDLNNHYPGVNALT
                     LSSIAIHLADKFDDKKDPDPDITRIRDEFVELKGALIFALEKQVSADNADYWTLISLA
                     ELRALTADNTATVVRSYRKALTASRRNMFFLQASLSQLEILKALDMRTTFVEAGISIL
                     DEEIARISGDKQVSAAKGNNKNGRAPEGQVFLFAGYMIDYPGKNKKTFPANRERDIRE
                     DIEKLLTKFNAGPGDIAILAGLSAGSEILFAEVCAEKGIHVNVHLPLPESRYIREFVS
                     IGGEQWVDRFYKICSHPDVDEFYQLEQVGSPKKNGDPYERNNRWALYSSLVRGISKTT
                     LIAVWNNVGGRAKDRDEYLTQHMVDLMRETGGKIEAINTTKYLFEGTAVPANATKPPA
                     PKKSKSSKK"
     gene            complement(1580..>1900)
                     /locus_tag="PKI33_12975"
     CDS             complement(1580..>1900)
                     /locus_tag="PKI33_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37975.1"
                     /translation="ATLTAKHGDMLLSELDSPNDVWPVNGEIFSETYMIIERGYCVKS
                     AVTMLVPMIDVTKGDEEQHVTIETMEGPQTVRAGDFYLARGVKGEIWSIPKEKVASIM
                     KPVE"
BASE COUNT          548 a          458 c          410 g          484 t
ORIGIN      
        1 aacgtgtaac cgttgcacag cgccaaaaac gtatcggcga catccttttg ctcacagaag
       61 agatcagcaa cccactaatc aaggaagagg cgattggtga agctggtaat gcccttgcaa
      121 atatgggtcg caacgagttg gcactcgttc agtatcgcaa aggcttggaa gtcaactctg
      181 gcaatctcga tttccgtcgt aaagaagctt tccacctcaa ccgccttggt cgtgtggatg
      241 aagccatcgt caagattgaa agtctgctta ccgattttcc aaatgatagc gaagccatct
      301 cataccttgg gcgcatctat aaagaaatgt gggcagattc ttggaaacaa attcccgata
      361 agaacaaacg ccttaaagcc gctttcgacg cctatcactg gcttatcaaa gcgatcgata
      421 cctatctcaa aggctatcgt atcgacttga ataatcacta ccctggcgtc aatgcattga
      481 ctctttcttc aatcgccatt cacctcgctg ataagttcga tgataagaaa gaccctgacc
      541 ccgatatcac ccgcatccgc gatgagtttg ttgagctaaa aggcgctctt attttcgcac
      601 tggaaaaaca agtgagtgct gacaatgcag attactggac tctgatctca ttagcagaat
      661 tacgcgccct tacagcagat aatacagcta ccgtagtgcg ttcataccgc aaagcgctca
      721 cggcatcgcg tcggaatatg ttcttcttgc aggcatcgct ttcccagcta gaaattctga
      781 aagccttgga tatgcgtaca accttcgtag aagctggcat tagcatttta gacgaagaaa
      841 ttgcccgcat ctctggcgat aagcaagtct ccgctgctaa ggggaacaac aaaaacgggc
      901 gcgcgcctga agggcaagtc ttcctttttg ctggctacat gattgattat cccggcaaaa
      961 acaagaagac cttccctgcg aatagagaaa gagatattcg tgaagatatt gaaaagttat
     1021 taaccaagtt caatgcaggt cctggcgata ttgctatttt ggctgggtta tcagcaggta
     1081 gtgaaattct atttgcagaa gtttgtgcag aaaaaggcat tcatgtcaat gttcatttgc
     1141 cgctcccaga gtcaagatac atccgcgaat ttgtttcaat cggtggcgaa caatgggtag
     1201 accgtttcta caagatctgc agtcatcctg atgtagatga gttttatcag cttgaacagg
     1261 ttggctcgcc caagaaaaat ggtgacccgt acgaacgcaa taatcgttgg gcgctttact
     1321 cttccctcgt gcgtggcatt tccaaaacga cacttattgc tgtttggaat aatgttggcg
     1381 gacgcgcaaa agaccgcgat gagtatctga cccaacatat ggtagatctc atgcgcgaaa
     1441 ctggtggcaa gatcgaagca atcaacacga ccaaatatct tttcgagggt acagcagttc
     1501 cagcgaatgc aacaaaacca cctgccccaa aaaaatcgaa atcttccaag aaataaaagc
     1561 aaaaatgaaa cccgtcgagc tactcgacgg gtttcattat ggatgctact ttttccttcg
     1621 ggatagacca aatttcaccc ttcaccccac gtgctaaata gaaatcgcct gctcgaaccg
     1681 tttgtggtcc ttccatggtt tcaatcgtta cgtgttgctc ttcatcgccc tttgtgacat
     1741 cgatcattgg aacaagcata gtcacagcac tcttcacaca ataaccacgt tcaatgatca
     1801 tatacgtttc agagaaaatc tcgccattta caggccaaac atcatttgga gaatccaact
     1861 cactaagaag catatcgccg tgtttagcag ttaaggtggc
//
LOCUS       DAOREF010000682         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_50530_length_1899_cov_4.000542, whole genome shotgun sequence.
ACCESSION   DAOREF010000682 DAOREF010000000
VERSION     DAOREF010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50530_length_1899_cov_4.000542"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1085
                     /locus_tag="PKI33_12980"
     CDS             273..1085
                     /locus_tag="PKI33_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37976.1"
                     /translation="MKKINADLLSLIVIAAFSFMFATAIHEHGGHALACVLQGGHISE
                     MGAFYVDCTRTSLSEFGNRVVAVAGPLVSFLTGLIALGYFYRNQRTDASLTFFLWHFA
                     TVNLMMASGYFLFSGVAGIGDLGTGESGAFQGVQPEWLVRMGLSVLGLVFYYAVIRIS
                     IKMMDHFIGGEGRERVGRAQMLSVIAYLVGGGIAILIGLFNPYGIVIVLVSSFASSMG
                     GTSGMAWMMQLLDRSKDTGMPAFAMPRNWSWIFVGVGFILLYAIIFGRTLYL"
     gene            1131..>1899
                     /locus_tag="PKI33_12985"
     CDS             1131..>1899
                     /locus_tag="PKI33_12985"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNM37977.1"
                     /translation="MKQNSMQHAWQQWAWVWHAIAYIMMALNLTIALGNSLRRGSFQT
                     TAWLTILLAVWYLPFMTLPARQSINNPWKLQLYFIIGWVIWMALIYFHNPAMMLAALF
                     YPQVFIRLPFRWAMVSAVLFTVSTFYLGFILPATPDALPTLYVVGVLLVSSQAILGGF
                     INSLISQSSQRYRLLNELEETRFNLLKAERETAILSERQRLSREIHDTLAQDFTSIIM
                     SLNAAKLTESSMAQVYLEQAEQTARDGLNDARRIVWAL"
BASE COUNT          448 a          391 c          448 g          612 t
ORIGIN      
        1 gaattaaagt atctatattt catcgtccca ccattcggta aaacactttc caatggttct
       61 tcaacgcccc agagggaacg aaatttgagg tagtccatgt gcgcctttac ttatatagaa
      121 gatttattaa gaagttggta aaactatata gttattttac ttgttctttt gtgctatcca
      181 tcgaaagatg gatagcaagg tcaatctcta gagagatgtg ttctaaaagc aatgagccta
      241 tactgcagcc attcttttta caggagcttt gtatgaaaaa aattaatgct gatttactgt
      301 ctctgatcgt tattgctgca ttctcattta tgtttgcaac agccatccat gaacacggtg
      361 ggcatgcact ggcatgcgta ttgcaaggcg gtcatatctc tgaaatgggt gctttttatg
      421 tggattgtac gcgaacatcc ttatctgaat ttgggaaccg tgtagtggct gtggctggac
      481 ctttggttag ttttctaact gggttgattg cattagggta tttctatcgt aatcagcgta
      541 cagatgcttc attgaccttt tttctatggc attttgccac agtcaactta atgatggcat
      601 ctggatattt tcttttctct ggtgtagccg gcatcggcga cttggggaca ggtgaaagtg
      661 gtgcatttca aggggtacag ccagaatggt tggtacgcat ggggctgtct gttttagggc
      721 tggtttttta ttatgctgtc atacgaatct caataaagat gatggatcat ttcattggtg
      781 gcgaaggtag agagcgagta gggcgcgctc aaatgctctc cgtaattgca tacctagtgg
      841 ggggtggtat tgctattttg atcggtcttt tcaacccata tggaatcgtc attgtattgg
      901 tctcatcctt tgcttccagt atggggggca cctctggtat ggcatggatg atgcagttgc
      961 ttgaccgttc aaaagacaca ggcatgcccg cgtttgcaat gccgcgtaat tggtcgtgga
     1021 tttttgtagg agtgggattt atccttttat atgccattat ttttgggcgt accttgtatt
     1081 tataaagcct gtgtacgaaa taacttttgc taatctctta taatgccagt atgaaacaaa
     1141 attccatgca gcatgcttgg caacaatggg cttgggtttg gcatgccatt gcctatatca
     1201 tgatggcatt aaatctcacc atcgcattag ggaattcctt gcgccgaggc tcattccaaa
     1261 ctacagcatg gcttactatt ttattggcgg tatggtacct gccattcatg acgcttcctg
     1321 cccggcaatc gatcaacaac ccttggaagc tgcagttgta tttcatcatt ggctgggtta
     1381 tttggatggc actaatctac ttccataacc cggctatgat gttggcggct ttgttttacc
     1441 cacaagtttt tatccgcctg ccatttcgct gggcaatggt cagtgctgtg ttgttcacgg
     1501 taagcacgtt ttatctcggg ttcattctgc ctgccacacc tgatgctttg ccaaccctgt
     1561 atgttgttgg ggttttattg gtttcttcgc aagctattct tggcggcttc attaactcct
     1621 tgatctcaca aagcagtcaa cggtatcgtc tgctcaatga gcttgaggag acgcgcttta
     1681 acctcctaaa ggctgaacgt gaaactgcaa tcttgagcga gcgccagaga ttatcacgtg
     1741 aaattcacga tacgcttgct caagacttca ccagtatcat tatgagtctc aacgctgcta
     1801 agttaactga aagcagcatg gcacaggtgt acttagagca agccgaacaa accgcacgcg
     1861 atggcttaaa cgatgcgcgt cgtattgtgt gggcattac
//
LOCUS       DAOREF010000683         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_50842_length_1893_cov_3.581066, whole genome shotgun sequence.
ACCESSION   DAOREF010000683 DAOREF010000000
VERSION     DAOREF010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50842_length_1893_cov_3.581066"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKI33_12990"
     CDS             complement(<1..763)
                     /locus_tag="PKI33_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37978.1"
                     /translation="MSKQTLKNNFFELEYLTHSLRISGITPHGKTNLLADLSHQPPVP
                     TPYGDFYFRGGHRLWHAPEAMPRTYVPDTGEIKITNIPNGVVLETETEPGANIRKRIE
                     IKLAVDTPSLTLTHTLFNDGLWTLELAPWAITQFRLGGIAILPMPTEKVDDAALLPNR
                     QISFWTYTRLNDKRLSLRDEFSLFHAESSLPPFKMGYFNPHGWLAYYIDGVLFKKTFN
                     ASTLATYPDNNCNAELFCNDEFVELESLGPLTKLEP"
     gene            complement(763..1737)
                     /locus_tag="PKI33_12995"
     CDS             complement(763..1737)
                     /locus_tag="PKI33_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNM37979.1"
                     /translation="MIYGEVEGIKKPIARVVQGTTMLGSDLNEEESFSLLDQVYELGG
                     NTLDTAHVYAGGNSERIIGKWMHSRGLREKMTIITKGGAHSQDRRRMTSFDVTSDLFD
                     SLARLKTDYIDLYLLHRDDTNLPVEPIIHTLNEHLQAGRIHAFGASNWSHQRMKDAAT
                     YAKANGLKTFTTSSPQYSLADSNAEPWPLCLSISGDTGKEAREWYANTAMPVLVWSPL
                     ASGFFSGRFRRDNLREFGEREWDEVVVRTYATEANFQRLDRAEALANEKGCTAAQIAL
                     AYVINQPMNVFSVVGSHSIEKFKANIEAAELKLTNQELAWLDLKVENL"
BASE COUNT          463 a          413 c          468 g          549 t
ORIGIN      
        1 cgggttccag ctttgtgagt gggcctaaac tttccaattc cacaaattca tcgttacaga
       61 aaagctcggc attgcaattg ttatctgggt aggttgcaag tgttgatgcg ttaaacgttt
      121 tcttgaagag aacgccgtct atataatatg ccagccagcc atgcgggttg aaatagccca
      181 ttttgaaagg cggcaaagaa gactcggcgt gaaaaaggct gaattcatcg cgcaggctta
      241 ggcgtttgtc gttgaggcgg gtgtaggtcc aaaaagatat ttggcggtta ggaagtaatg
      301 cggcatcatc cactttttca gtgggcatgg gcaaaattgc tattccacct aatcgaaatt
      361 gagtgatcgc ccatggtgcc agttcgagag tccacaagcc gtcattgaaa agggtgtggg
      421 tgagggtgag agagggtgta tctactgcaa gtttaatttc gatccgcttg cgaatgttgg
      481 caccgggctc tgtttcggtt tcaagcacaa caccattggg aatattagtg atctttattt
      541 caccagtatc ggggacataa gtgcgcggca tggcttcggg ggcatgccat aaacgatgcc
      601 cgcctcgaaa atagaaatcg ccgtaagggg ttgggacggg cggctgatga cttagatcgg
      661 ctagcaaatt ggttttgcca tgcggggtta tgccagagat acgtaaagaa tgtgtgagat
      721 attcaagctc aaagaaattg ttttttaggg tttgtttgct cattatagat tttctacttt
      781 caggtccagc caagccaatt cttggttggt gagtttcaat tcagcggctt caatgtttgc
      841 tttgaacttt tcaatactat gagaaccaac aactgaaaaa acattcatcg gttggtttat
      901 tacatacgct aatgcgattt gcgcggctgt gcatcctttt tcatttgcca aagcttcagc
      961 acggtctaaa cgttgaaagt ttgcttcggt ggcatatgtt cgcacaacta cttcatccca
     1021 ttcgcgttca ccaaactcgc gcaggttgtc tcttcgaaaa cgcccagaga aaaacccact
     1081 ggcaagtgga gaccacacca aaaccggcat tgcggtgttg gcataccatt ctctggcttc
     1141 tttgccagtg tcaccgctaa tgctcaaaca taagggccaa ggttcagcgt ttgagtctgc
     1201 taggctgtat tgtgggctgc tggttgtgaa tgttttcaag ccgttggctt ttgcataggt
     1261 tgcagcatct ttcatccgtt ggtgagacca gttcgatgcg ccaaacgcat gaatgcgacc
     1321 cgcttgtaaa tgttcattga gtgtgtgaat aatcggctca accggtagat tggtatcatc
     1381 acgatgcaac aggtaaaggt caatgtaatc tgtcttgagg cgtgcaagtg agtcgaatag
     1441 atcagaggta acatcaaaag acgtcattct gcggcggtct tgtgaatgtg cgccgccttt
     1501 tgtaatgatg gtcattttct cacgcaatcc gcgcgagtgc atccattttc caataatgcg
     1561 ttcgctgttt ccaccagcgt acacatgcgc tgtatcgagt gtgttaccgc caagttcata
     1621 tacttgatct aaaagagaaa aactttcttc ctcattcaaa tcactgccca acatagttgt
     1681 tccttgcaca acacgggcaa ttggcttttt tatgccttca acctcgccat agatcatcaa
     1741 atacctctgt cgattggatt tgttttacca ataattgtga acgagactgc ggatctgaga
     1801 gtcgtacacg gctttgattt tttccatcgc ctctgggctc agggacggct ggtctgccgc
     1861 cttgacatta tcttccgctt gagccggatt ctt
//
LOCUS       DAOREF010000684         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_50943_length_1891_cov_4.315904, whole genome shotgun sequence.
ACCESSION   DAOREF010000684 DAOREF010000000
VERSION     DAOREF010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50943_length_1891_cov_4.315904"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1673
                     /locus_tag="PKI33_13000"
     CDS             318..1673
                     /locus_tag="PKI33_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37980.1"
                     /translation="MADAFLQGHTYLETPVNPLLLELPDPYNPGARGNLEFLWDTSLY
                     EGKYYLYWGPMPSVIIMVIKLIYPGFIGDNLVVFAAVAIITIFQTLLLTWLWTQYFQR
                     LPIWTLFIGVLMAGLIIPLNIMNNRPEIYEGAIISGQAFLMGGIYFAVLAVRPTEIFP
                     RHLVAASMLWGCAVASRTIVVFEVIFASMLIGLLILNRPVGWAWRLKRLIAFGLPLAL
                     FAFGLGAYNFVRFGSAFEFGYKYQLTMFDFSKIGDELFSTRYMFPSMENYVLTAPHRI
                     KTFPYFRPKAGDEIEFSVKSPDLYYIETITGVIYTFPFVIFALVPILRTLTKGVKLGK
                     NTDTSEYILQWVVVLLSGMGVIAVTQLLSFFYTTMRYLADVTPILSVLALMGFWFGYQ
                     TVSSNKALRFAYAGIGVFLAGISIIVPNMLALVISERISTYSPNVLPALDALFKSVFL
                     K"
BASE COUNT          475 a          365 c          435 g          616 t
ORIGIN      
        1 cgaggggagt ggaaagtgga aagtggatag aaagtgcgac gcaggaagtg attacgttgt
       61 tgagggatga ttacccgacg ttgatgtccg tgttgggggc gcttagcaat gtataaatct
      121 actatggaat aaataaacct ataaactata tgatgttaag aaatgaaatt tcgccaacca
      181 tacgaataaa gcttgtgtat gcatctgcag ctcttgcatg tgcgtctgct cttgttgtac
      241 aaatctggtt tgccacagca ggtacatggt ttacattgcc aacgttccca tacccttacg
      301 atcattacta caatcaattg gcagatgcat ttcttcaagg gcacacctat cttgagactc
      361 ctgtcaatcc tttacttctt gaactcccag atccttataa cccgggagca cggggcaacc
      421 ttgaattttt atgggacacg agtttgtatg aagggaaata ttatttgtat tggggaccaa
      481 tgccctcggt cattattatg gtgatcaagt taatatatcc gggttttatt ggcgataatt
      541 tggtcgtgtt tgctgcggta gctattatta cgatatttca aactttattg cttacgtggc
      601 tttggaccca atatttccaa cgtttgccta tttggacact tttcataggc gttttgatgg
      661 ctgggttgat aattcccttg aatattatga ataaccgtcc ggaaatttat gagggtgcta
      721 tcatcagcgg acaggctttt ttgatgggag ggatctattt tgcagtgctt gccgttcgcc
      781 ctactgaaat ttttccacgc catctggttg cagcatctat gctttgggga tgtgcggtcg
      841 cctcacgtac catcgtcgtc tttgaagtaa tctttgcatc aatgttgatt ggacttctaa
      901 tactgaaccg ccctgttggg tgggcttgga gacttaagag actcattgcc ttcggtctgc
      961 cactggcatt gtttgcgttt ggtttgggag cctataactt tgtacgcttt gggtctgcct
     1021 ttgagtttgg gtataaatac caactaacca tgttcgattt ttcaaaaatt ggagatgaat
     1081 tattctccac ccgatatatg ttcccaagta tggaaaatta tgtcctaact gcaccgcacc
     1141 gcattaaaac gtttccctat tttagaccca aagcgggaga tgaaattgaa ttttctgtta
     1201 agtcgccaga tttgtattat atagaaacga tcactggtgt gatttacacc ttcccttttg
     1261 taatttttgc tcttgtgccg atcttaagaa cgcttacgaa aggagtcaag ctgggtaaaa
     1321 atacggatac atcagaatat attctgcaat gggttgttgt tttattaagt ggtatggggg
     1381 tgattgccgt cacccagctt ctgtctttct tttatacaac gatgcggtat cttgcagatg
     1441 tgacaccgat cttatctgta ctggcattga tgggtttttg gtttggatat caaacagttt
     1501 cttcaaataa ggcactaagg tttgcctacg ctggcatagg tgtttttttg gcaggaatta
     1561 gcataatcgt ccctaatatg cttgctttgg ttatttcgga aagaatcagt acgtatagtc
     1621 caaatgtgct acctgccttg gatgccttat ttaagtctgt ttttctaaaa tgaaataatt
     1681 caaatgaagc aatctcatga ctagcgtagg agattgcttc gtcgcgaaga gcaagagcgc
     1741 tcctcgcaat gacgaggaat gaagaggttt ttatgaacat acaacgaact caaattggct
     1801 acctcgtcgg tggcggactt aaagaaaatt tccgcgtgcg tcttaccgtt tctccgcagg
     1861 agattcagga gggggcgttt gtcgtcatcc a
//
LOCUS       DAOREF010000685         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51012_length_1890_cov_3.256131, whole genome shotgun sequence.
ACCESSION   DAOREF010000685 DAOREF010000000
VERSION     DAOREF010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51012_length_1890_cov_3.256131"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..586
                     /locus_tag="PKI33_13005"
     CDS             164..586
                     /locus_tag="PKI33_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNM37981.1"
                     /translation="MSISSEAWLEQLHENGYRITAARRAVVDTVFGNTHALTPIEVYD
                     AARKKYRALGLVTVYRTLEKLEELHLIQRVHQPMGCQAFIAAGVGHQHLLLCQQCGQV
                     EFFEGDDLEPLTKSIAKKTGYQIQEHWLQLFGLCTNCK"
     gene            671..1609
                     /locus_tag="PKI33_13010"
     CDS             671..1609
                     /locus_tag="PKI33_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNM37982.1"
                     /translation="MKKIFNTIIVTLTFSALLLAGCGSAPQSGSDTLKVLASTTFLAD
                     IAQNIAGDRVQVESLLPVGADPHAYQAAPADVSKIAESNVLILNGVEYEHFIEPLLEN
                     AGGERLVIEATTGLTPHQMEEHAAEAESGTSHEHEAGDPHMWLDPNLVVTYVENIRDG
                     LSKADPDGAETYAANAEAYIAQLKDLDAWISEQVNSIPAENRLLVTNHEALGYFAERY
                     GFEVVDTILPSFSSEASASAQEIAAAVDAVKNSGAPAIFLGEVENADLANQIASETGV
                     KVVNTLYLESLTDGAPAPTYIDMMKHNVTEIVNGLK"
     gene            1678..>1890
                     /locus_tag="PKI33_13015"
     CDS             1678..>1890
                     /locus_tag="PKI33_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM37983.1"
                     /translation="MSHTPHRLEVKNISIGYGDKTILQDLSFQVPHGSRVAVVGPNGA
                     GKSTLFKALVGLLPLQHGDIIIHGESL"
BASE COUNT          502 a          457 c          467 g          464 t
ORIGIN      
        1 tagatagcgg cgaagtgaac gatctgggaa tagacggttt cacccccaag tgggtttggt
       61 aataccaagt gacagtcacc ttaaaggtga ctgtcacttt tttcttgact cttcaaaaaa
      121 catcctttat actaattgaa aatgattttc agtaaggaaa cccatgtcca tctcttctga
      181 agcatggctc gaacaactgc atgaaaatgg ataccgcatc acagccgctc ggcgtgccgt
      241 ggtggatacg gtctttggca acacccatgc cctgactccc atcgaagtct atgatgcggc
      301 gcgcaagaag tatcgcgctt tgggactggt cactgtttat cgaacgctcg aaaaactcga
      361 agagttgcat cttatccaac gcgtgcatca gcccatgggt tgtcaggcat tcatcgcggc
      421 aggtgttggg catcaacacc tcttgctttg ccagcaatgc gggcaggttg agttcttcga
      481 aggcgatgat cttgaaccct taaccaagtc tattgccaaa aagaccggtt atcaaattca
      541 ggaacattgg ctgcaactat ttggcttgtg tacgaattgc aaataaattt tgctcatgat
      601 gtgaattagc cccatctgag tgataaaaaa actccctcac atgttgaggg ataaatttta
      661 ggagttctaa atgaaaaaga ttttcaatac cattatcgta acattaacct tctcggctct
      721 tctcctcgcc gggtgcgggt ctgccccaca aagcggaagc gacaccttga aggttctcgc
      781 ctctaccacc ttcctggcag acattgccca gaacatcgct ggcgaccggg tacaggttga
      841 gtccctgttg cctgttggcg cagatccgca tgcctatcag gctgccccag ccgatgtttc
      901 caagatcgcg gaaagcaatg tgttgattct caatggcgtg gagtatgagc actttatcga
      961 gccgttgctg gaaaatgcag gcggagaacg cttggtcatt gaagccacca ccgggctcac
     1021 cccgcatcag atggaagaac atgccgccga agcagaatca ggcacaagtc acgagcatga
     1081 ggcgggcgat ccgcatatgt ggctggaccc gaaccttgtc gtcacttatg tagagaatat
     1141 ccgtgacggc ttgagcaaag cagaccctga cggtgcagaa acctatgccg ccaatgccga
     1201 agcctatatt gcgcagttga aagacttgga tgcatggatt agcgaacagg taaacagcat
     1261 tccggcagag aatcgtttgt tggtcacaaa ccatgaggcg ttgggttatt ttgcagaacg
     1321 ctatggcttt gaagttgtgg ataccatctt gccgagcttt agttctgaag caagcgcttc
     1381 tgcccaagag atcgctgcag cggtagatgc tgttaagaat tctggcgcgc ctgccatttt
     1441 cctcggcgaa gtagaaaatg cagacctcgc gaatcagatc gcttctgaaa ccggggtgaa
     1501 ggtggtcaac actctttatt tagaatcgtt gaccgacggc gccccagccc caacctatat
     1561 cgacatgatg aagcacaatg tgactgaaat cgtgaatgga ttgaagtaag atagaagtga
     1621 aataagcgcg tctctcaagt gccaatccaa aatcgtaaat caaaaatcga aaatcaaatg
     1681 tctcacacac cccatcgtct tgaagtaaaa aacattagca tcggctacgg tgacaaaact
     1741 attttgcagg atttgagttt tcaagtgccg catgggtcac gtgttgccgt ggtcggtccg
     1801 aacggagcgg gaaagtccac gctgttcaaa gctctggttg gtctactgcc cttacaacat
     1861 ggcgatatca tcattcatgg cgagtcgctt
//
LOCUS       DAOREF010000686         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51107_length_1888_cov_4.078560, whole genome shotgun sequence.
ACCESSION   DAOREF010000686 DAOREF010000000
VERSION     DAOREF010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51107_length_1888_cov_4.078560"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKI33_13020"
     CDS             complement(<1..373)
                     /locus_tag="PKI33_13020"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNM37984.1"
                     /translation="MTETNSNANRQIARAAGTVMLAFVLSNIMGLVRQILVSKTFGTN
                     QSLDAFYAAATFPDLLFSLLAGGALASAFVPTFTGFLANEKKEDAWRLASAVTNLVIL
                     LLSAVSGLAALFAPGIVRHVLF"
     gene            398..1516
                     /locus_tag="PKI33_13025"
     CDS             398..1516
                     /locus_tag="PKI33_13025"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNM37985.1"
                     /translation="MKKTSTSLFLLVILSLACSSTTLTPDVAPQTPPTVDSTDPASAT
                     LTEIPLPVGYGAKNSWIEIYFTDPSNSLSSQKTGGIDQPLADAIDSAKLTVDVAIYSL
                     SLNSIRDALLRAQDRGVQVRMVMESENLDRSDPQRLLDAGIPILGDRREGLMHNKFTV
                     IDGSEVWVGGMNYTDSGTYEDNNVLMRIRSTKIAENYTKEFEEMFVDDKFGDNVLAET
                     PNPRVTMDGTPVDTYFSPDDGVQSILLDILNEAQSSIYFMAFSFTSDELGDAILQRAE
                     SGVTVAGVMDEEQVQSNQGTEYDAFRRAGLDVYIDGNPGQMHHKIMIVDESIVIVGSY
                     NFTNSAETRNDENLMVIYDKDIAAQFLNEFQRVYGQAK"
     gene            1640..>1888
                     /locus_tag="PKI33_13030"
     CDS             1640..>1888
                     /locus_tag="PKI33_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNM37986.1"
                     /translation="MKKQSYIFLVLVALIAVFVSACGGAAAATDTSAGSDKPRASLVE
                     FTGVIEAIDGNQWTVNGQVITVDPTVLRDGPFVVGDTVK"
BASE COUNT          493 a          443 c          483 g          469 t
ORIGIN      
        1 cgaatagaac atggcgaaca attcccggcg cgaagagagc cgcgagtccg cttactgcgc
       61 tgagcaacag aatcaccaaa ttcgtcaccg cagaggcgag ccgccaggca tcctcttttt
      121 tttcgttggc gagaaaacca gtaaaagttg gcacgaacgc cgaagccaaa gccccgccag
      181 ccagcaaact aaaaagcaaa tccgggaagg tggctgcggc ataaaacgca tcgagacttt
      241 gattagtgcc gaaagttttt gaaacaagga tctgacgcac cagccccatg atgttgctca
      301 gtacaaaagc gagcatcacc gttccggcgg cacgtgcgat ctgacgatta gcattcgaat
      361 tggtttctgt catggcggca agattataat gtttttcatg aagaaaacat cgacaagcct
      421 ctttctcctt gtgatccttt cactggcgtg cagttctact actctcacgc cagacgttgc
      481 gcctcaaacc ccgccgactg ttgactctac agacccggca tctgccacac tgacggagat
      541 tcccttacca gttgggtatg gtgccaaaaa ttcgtggatc gaaatttatt tcaccgaccc
      601 atcgaattca ttatcgtctc aaaagactgg cggcattgac caacccttgg cggatgccat
      661 cgactctgcc aagttgacgg tagatgttgc gatttatagt ttgagtctga atagcattcg
      721 tgatgcgttg ttgcgtgcgc aagatcgtgg cgtgcaagtg cgcatggtga tggaaagcga
      781 gaacctcgac cgttctgacc cgcaacgttt gcttgatgcg ggcataccta ttttgggaga
      841 tcgccgcgaa ggcttgatgc acaataagtt tacggtcatt gatggttctg aggtatgggt
      901 ggggggcatg aactataccg actcaggaac atacgaagat aacaatgtgc tgatgcgcat
      961 ccgctcgaca aagatcgctg aaaattacac caaagaattc gaagaaatgt ttgtggatga
     1021 taaatttggc gataatgtcc ttgccgaaac gcccaatccg cgtgtgacga tggatggtac
     1081 gccagtggat acgtatttct cgcccgatga tggtgtgcaa tctattttgc tcgatattct
     1141 caatgaagct caatccagca tttactttat ggcgttttct tttacatctg atgaattagg
     1201 cgatgccatc cttcagcgtg cagaatccgg cgtcactgta gcaggggtga tggacgagga
     1261 acaagtccag tcgaatcaag gaaccgagta cgatgccttc cgtcgagccg ggttggatgt
     1321 gtatatcgac ggcaaccccg ggcagatgca tcacaaaatc atgatcgtgg acgaaagcat
     1381 cgtgattgtc ggttcgtaca attttacgaa tagcgccgaa actaggaatg atgagaattt
     1441 gatggtgatc tacgataaag acattgcggc tcagttctta aatgaatttc agcgagtgta
     1501 tggtcaggca aaatagactt taacctgtat tttattcttt acggctcgtc cctaaaattg
     1561 cacaggggcg agccgtaact tttatgtacc ctctacgact atctgggtgt agccaaataa
     1621 aaaaagaaga ggtgtaaaca tgaaaaagca atcttatatc ttcctggttc tggtagcgtt
     1681 gatcgcagtt tttgtcagtg cctgtggtgg ggcagccgcc gccaccgata catcggcggg
     1741 ttcagacaag ccgcgtgcgt cactagttga atttactggc gtgatcgaag ccatcgatgg
     1801 taaccagtgg accgtcaatg gacaggtcat caccgtggac cccacagttc tgcgcgatgg
     1861 tcctttcgta gtgggcgata ccgtcaaa
//
LOCUS       DAOREF010000687         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51108_length_1888_cov_4.058920, whole genome shotgun sequence.
ACCESSION   DAOREF010000687 DAOREF010000000
VERSION     DAOREF010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51108_length_1888_cov_4.058920"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKI33_13035"
     CDS             <1..268
                     /locus_tag="PKI33_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNM37987.1"
                     /translation="NAEQFCPERFAHGRKTPPMSYIPFGGGPRACIGAAFGQAEARIV
                     LAHLLKNFTFEFTGHTIHAHMGATLEPRPGVKMKVKRRTVASVQ"
     gene            334..1128
                     /locus_tag="PKI33_13040"
     CDS             334..1128
                     /locus_tag="PKI33_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNM37988.1"
                     /translation="MTYFGFLLRFLVIPILVFLVITWWDNKNNRPTFGFNNGKAVWVA
                     ILIHIVLAVVYTTPWDNYLVATGVWYYNPNLVTGIVIGYVPIEEYTFFVLETILAGLW
                     WWFLARRIPEPTNTFKPNKTGRIISFSVLFIFWVIFTYLFFFGDVELTYLSIILFWAF
                     PAIFPQMLYGADILWHYRKLVFITIFVPGLYLSLMDIIALTDTTWSISKEQTTGILFF
                     NILPLEEVLFFFITNVLIGFGMTLLLSKLGTQRFEDWKSNNYKGLP"
     gene            1125..1499
                     /locus_tag="PKI33_13045"
     CDS             1125..1499
                     /locus_tag="PKI33_13045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNM37989.1"
                     /translation="MKNIEQFKKQQYINLETFRKNGGGVKTPVWFAQDGETLRIWTQA
                     DAGKTKRIRRDGKVRIAPSTASGEPIGEWIDANATVLNTPDEINYTKDLFRKKYGVMF
                     NVFGFLGKVRGSSFVTIKVNLN"
     gene            1582..>1888
                     /locus_tag="PKI33_13050"
     CDS             1582..>1888
                     /locus_tag="PKI33_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37990.1"
                     /translation="MINQMEQNILNLLQHLYDELGWFGVTIIMIVENSTGIAPSEVVL
                     ALAGWMLIDAHSLPFSTVFLGGLFAAFGSVMGASILYWAARLGGRPVVERVAKFFRID
                     "
BASE COUNT          491 a          450 c          403 g          544 t
ORIGIN      
        1 aaatgctgag caattttgcc ccgagcgttt cgcccatgga cggaaaactc cgcctatgag
       61 ttacattccg tttggcggcg gtccacgggc ttgcattggc gcggcattcg gtcaggcaga
      121 ggcgcggatc gtcctcgctc acttgctcaa gaacttcaca ttcgaattca ctggtcatac
      181 tattcacgcg cacatgggtg ccacactgga acccagaccg ggcgtaaaga tgaaagtgaa
      241 gagaaggact gtagccagtg tgcagtgaag tatgacttat acaaacgact atctgactaa
      301 caaactatta gactaagtga ctaacaaact acaatgacat actttggttt tctcttacgc
      361 tttctcgtca ttcccatcct tgtttttctc gtcatcacat ggtgggataa taaaaacaat
      421 cgccccacat ttggttttaa caatggtaaa gccgtatggg tcgcgattct gattcatatc
      481 gtccttgccg tggtgtatac cacgccatgg gataactatc tagttgccac cggcgtttgg
      541 tattacaacc ccaatcttgt gacaggcatt gtcattggtt acgtgcctat tgaagagtac
      601 accttctttg tgctagaaac catcctcgcc gggttatggt ggtggttcct tgcacgtcgc
      661 atccctgaac cgacgaacac attcaagcct aacaaaaccg ggcgcatcat ctcattcagt
      721 gttctgttta ttttctgggt catcttcacc tatctgttct tcttcggcga tgtggaattg
      781 acctatctct caattattct gttctgggcg tttcctgcca tcttcccaca aatgctgtat
      841 ggcgccgata tcctctggca ttaccgcaag ttggttttca tcacgatatt tgtcccaggc
      901 ttatatcttt ctctaatgga tattatcgct ctcacagaca ccacctggtc tatttccaag
      961 gaacagacca caggtattct cttctttaat attctgccat tagaagaagt tttattcttt
     1021 ttcatcacca acgtcctcat tggctttggc atgacattgc ttctttcaaa acttggcacc
     1081 cagcgctttg aggattggaa atcaaacaac tacaaaggtc taccatgaag aacattgaac
     1141 aattcaaaaa acaacaatac ataaaccttg agacgtttcg caaaaatggc ggaggagtca
     1201 aaactccagt ttggttcgca caggatggag aaacgcttcg tatctggacc caagcggacg
     1261 ccggcaaaac caagcgcatc cgccgggacg gcaaagtgcg catcgcacca tccacagctt
     1321 caggcgaacc tattggcgaa tggattgatg ccaatgctac agttcttaat acaccggatg
     1381 aaataaacta cacaaaagac ctttttcgca aaaagtacgg agtaatgttc aatgtatttg
     1441 gctttttagg aaaagtccgc ggctcaagct tcgttacgat caaagtaaat cttaactaat
     1501 ttttctctca ttaggattaa accatggctc atctttttta tgtaagatag aagccagttt
     1561 agatctagag acaagaatag aatgatcaat caaatggagc agaatatctt aaatttactt
     1621 caacatctat atgatgagct aggttggttt ggcgtcacca tcatcatgat tgttgaaaac
     1681 tcaactggca tcgcccctag tgaggtcgtg cttgcccttg ctggctggat gttgattgac
     1741 gcacatagtt tgcctttttc taccgtgttt cttggagggc ttttcgccgc attcggaagt
     1801 gtaatgggag catcgatctt atactgggca gcacgcctcg gcggacgccc cgtggtagag
     1861 cgggttgcca aattctttcg tattgatt
//
LOCUS       DAOREF010000688         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51162_length_1887_cov_3.323144, whole genome shotgun sequence.
ACCESSION   DAOREF010000688 DAOREF010000000
VERSION     DAOREF010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51162_length_1887_cov_3.323144"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKI33_13055"
     CDS             <1..270
                     /locus_tag="PKI33_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNM37991.1"
                     /translation="LTPCNECHAGLLHPRHVTYFTWLGHELITVPHFPAWVCDVCGRR
                     EYDERAIQWLNMILDPNAGKPTPSHRNTPPRPRPQTGFRSPTPES"
     gene            285..842
                     /locus_tag="PKI33_13060"
     CDS             285..842
                     /locus_tag="PKI33_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37992.1"
                     /translation="MSLDSTSPRTYRFVLADILTSGYRVAGKVAVTTHGAMGFLNDIN
                     HSTLEIHDARMARLHMPTKLVDHFELVRTMKSRIHAVCLPRREDLGPQGIVRGGYMNV
                     VEYQVRLTTSMFEVDGIMELPGRFDFAGLISDGTREFLPIFNATVTAILIPSLRVQSA
                     GVLINRKMVDLVALLNQRVKPETHP"
     gene            896..968
                     /locus_tag="PKI33_13065"
     tRNA            896..968
                     /locus_tag="PKI33_13065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:930..932,aa:Glu,seq:ttc)
     gene            1406..>1887
                     /locus_tag="PKI33_13070"
     CDS             1406..>1887
                     /locus_tag="PKI33_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNM37993.1"
                     /translation="MLDMNLLRENPEIVRKALLDRQDNPAPVDSILALDEKRRGLLAQ
                     VEQLKAERNVVSKEIGKMKDATERQSKIEAMRVVGDQITELDKLVAEVESELNYLMST
                     LPNIPDPRTPYGKDDSENIVIKTVGEPRKFDFTPLPHWDLGPALGIIDFERGTKLTGS
                     R"
BASE COUNT          455 a          490 c          508 g          434 t
ORIGIN      
        1 ttaactcctt gtaacgaatg ccatgcgggt ttgctgcatc cccgtcatgt gacttacttc
       61 acctggctcg ggcatgagtt gatcactgta ccgcatttcc cggcttgggt gtgtgatgtg
      121 tgcgggcggc gcgagtatga tgaacgcgcg atccaatggt tgaacatgat cttggacccg
      181 aatgccggca agcctacgcc ttcgcatcgc aacaccccgc ctcgccctcg acctcaaaca
      241 gggttccgct ccccgacccc tgaatcttaa ctcaaggttt ctccatgtca cttgatagca
      301 cttctcctcg tacatatcgc tttgtgctgg cagatatcct aacttctggg tatcgtgtgg
      361 caggcaaggt tgcagtgacc actcatgggg ccatggggtt cttaaacgat atcaatcatt
      421 caacgctgga aattcatgat gcgcgtatgg cacgtttaca catgcctacc aagttagtgg
      481 atcatttcga attggtacgc acgatgaaga gccgcattca tgctgtttgt ttacctcggc
      541 gtgaagacct gggtccgcaa gggattgtgc gtgggggata tatgaatgta gtggaatatc
      601 aggtgcgcct gacgacttcc atgtttgaag tggacggtat catggagttg cccggtcgct
      661 ttgattttgc cgggctgatc tcagatggca cgcgtgaatt cctgccgatt ttcaatgcta
      721 cagtgacggc gatcctcata ccaagcttgc gagtgcaaag cgcaggtgtg ctgattaacc
      781 gcaaaatggt agacctggtg gcgcttttga accagcgtgt gaagccagaa actcatccct
      841 aaaatttctt gacgcatccc gaagtggatg ataaaatctc gcccgcttaa gaaatgcccc
      901 catcgtctag tggactagga cgcagccctt tcaaggctac gaccggggtt cgaatccccg
      961 tgggggcaca acctaaaagc aaccgtctcg taagaggcgg tttttagttt aaaaagccag
     1021 actcattttt ggtataatcc cgtcagttcg aaatcaaaga caagcgcaaa gataagtagt
     1081 tcagcaacgg tacagagagc cgccggtgag tgcgaggcgg accgccatag gatgaaatcc
     1141 actttgccaa cgttgttctc ggagagacgc aagtcataag agaatcggga gcgcccgtta
     1201 gcgcgcatcc aaggttatgc ttcggcatag cgaaagcagg tggcaccacg agacgcccct
     1261 cgtcctgcaa atggaatggg gcgtatttta tttaatgtct tcgaatgtac gggcgaccct
     1321 tccatcggac gcaagtctac gaattcgatc ttggcgggtc gcccctacaa cggaaggcaa
     1381 acaataatgc actcggaggt gcaacatgct cgatatgaat ttgcttcgtg aaaacccaga
     1441 gatcgtccgc aaggcgctgc tcgaccgaca agataaccca gctccggtcg attctattct
     1501 ggcgttagac gaaaagcgcc gtggattgct ggctcaggtt gagcagctca aagcggagcg
     1561 caatgtcgtc tcgaaagaga ttggcaaaat gaaagacgca accgaacgcc agtccaagat
     1621 tgaggcgatg cgcgtggtgg gcgatcagat cacagagttg gataaactcg tggcagaggt
     1681 tgaatccgaa ttgaattacc tcatgtccac gctgccgaac atccctgatc cgcgcacgcc
     1741 ctatggcaag gatgacagcg agaacatcgt catcaagacg gtgggggagc cgcgcaagtt
     1801 tgacttcacc ccgcttccac actgggacct cggacctgcg ctcggcatca ttgactttga
     1861 acgcggcacc aagctgaccg gttcccg
//
LOCUS       DAOREF010000689         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51299_length_1884_cov_4.124112, whole genome shotgun sequence.
ACCESSION   DAOREF010000689 DAOREF010000000
VERSION     DAOREF010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51299_length_1884_cov_4.124112"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKI33_13075"
     CDS             <1..351
                     /locus_tag="PKI33_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNM37994.1"
                     /translation="VLGKQLKSPILTNDYNLNRVAELQGVTVLNINELANAVKSVVLP
                     GELLRINIMQEGKEHGQGVGYMDDGTMVVVENGRDFIGEYMEVNITKVLQTAAGRMIF
                     GRVDEENSKKSKNK"
     gene            500..>1884
                     /gene="cysS"
                     /locus_tag="PKI33_13080"
     CDS             500..>1884
                     /gene="cysS"
                     /locus_tag="PKI33_13080"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNM37995.1"
                     /translation="MLKIYNTLTRKTEEFQTLEPNKVKMYVCGVTVYNDAHVGHAMSA
                     IVFDIVRRYLEYRGYAVKHVMNYTDVDDKIINRANQLGEDPLKLSARYIADYAADLKS
                     LNVLPATSNPQVSTTMPLIIQFIEGLIQKGHAYAAENGDVYFRVTSDDDYGRLSGRKL
                     EDMQAGARIEVGEQKEHPMDFALWKAAKPGEIFWESPWGKGRPGWHIECSAMNLAELG
                     EQIDIHGGGNDLIFPHHENEIAQSESYTGKEFSRYWIHNGMLQLGGEKMSKSLGNIIS
                     IKEFLSKRSSDVMRMLVLNGTYRAPLMFNDETLDAAERNVERLKSALKPASPSASGLS
                     ADTLSVSVESSKTNFTEAMDNDFNTAGAVAALFELSKSINTARDNGATADQLKPAQET
                     FKQLAGVLGLKLEEKKGSSEDEAQVEALLAERTQARKDKNWKRSDEIRDQLKAMGVTI
                     EDSKDGTTWKW"
BASE COUNT          538 a          538 c          440 g          368 t
ORIGIN      
        1 gtactcggca agcaactcaa gagtcccatt cttaccaacg attacaacct caatcgcgtc
       61 gctgaattac aaggggtaac cgtcctcaat atcaatgagt tggcaaatgc ggtcaaatcg
      121 gtagtgttgc ctggcgaact cctgcgcatc aatattatgc aggaaggcaa agaacacgga
      181 caaggcgtag gctacatgga cgatggtacg atggtcgtcg tcgaaaatgg acgtgatttc
      241 attggcgaat acatggaagt gaacatcacc aaggtccttc agaccgcagc cgggcgtatg
      301 atcttcgggc gtgtagacga agagaatagc aaaaaatcta aaaacaagta ataagcaaca
      361 agggacgagt agtcagtgaa caggacgtgt gaagcgtcct gtttttttgt cccaatgcgg
      421 taaaatacaa ccgtcttaaa aattcctttg tgatctcaag ccctcgaata agggcatggc
      481 acttggtggt aaaacaaaca tgttgaaaat ttacaacact ctcacccgca aaaccgaaga
      541 attccaaacc ctcgaaccga ataaagtcaa aatgtatgtt tgtggcgtca cggtctataa
      601 cgacgctcat gttgggcatg ccatgtctgc catcgtgttc gatatcgtcc gccgctacct
      661 cgaatatcgc ggatacgccg tcaaacacgt gatgaactac accgacgtag acgacaaaat
      721 catcaaccgc gccaaccaac ttggtgaaga ccctctcaaa ctttcggcgc gttatattgc
      781 tgattatgcc gccgacctca agagtctcaa tgttctgcct gccacgtcca atccgcaggt
      841 cagcaccacc atgccgctca tcatccaatt catcgaaggg ctgatccaaa aaggtcacgc
      901 ctacgccgca gaaaatggcg atgtctattt ccgcgtcacc agcgacgatg attatggtcg
      961 cctctcagga cgcaagcttg aagacatgca ggccggcgca cgcatcgaag ttggcgaaca
     1021 aaaagaacac ccgatggatt tcgcgctctg gaaagccgcc aagcccggcg aaattttctg
     1081 ggaaagccca tggggcaagg gccgccccgg ttggcatatc gaatgctctg ctatgaacct
     1141 cgccgaactt ggcgaacaga tcgacatcca cggcggcgga aacgacctca tcttccccca
     1201 ccacgaaaac gaaattgccc aaagcgaaag ctacaccggc aaagaattct cgcgctactg
     1261 gatccacaac ggcatgctgc aactcggcgg cgagaaaatg tccaagtctt tgggcaatat
     1321 catcagcatc aaagaatttc tctccaagcg ttcttccgat gtcatgcgca tgttggtctt
     1381 gaacggaacc tatcgcgccc ctctgatgtt caacgacgaa acgctggatg ccgctgaacg
     1441 caacgtcgaa cgcctcaaat ccgcgctcaa gcctgcctcc ccctccgcca gcggactctc
     1501 cgctgatacc ttgtccgttt cggtcgaatc ctcaaaaacc aacttcaccg aagccatgga
     1561 caatgacttc aacaccgccg gagccgtcgc tgcgttattc gaactctcca agtccatcaa
     1621 caccgcccgc gataacggcg cgactgccga tcaactcaaa cccgcacaag agactttcaa
     1681 acaattggct ggcgtactcg gtttaaaact cgaagagaaa aaaggttcca gcgaagacga
     1741 agctcaagtg gaagccctcc tcgccgaacg cacccaagcc cgcaaagaca aaaactggaa
     1801 gcgctccgac gagatccgcg accagctcaa agcgatgggc gtcaccatcg aagacagcaa
     1861 agacggcacc acctggaagt ggag
//
LOCUS       DAOREF010000690         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51316_length_1884_cov_2.727720, whole genome shotgun sequence.
ACCESSION   DAOREF010000690 DAOREF010000000
VERSION     DAOREF010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51316_length_1884_cov_2.727720"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..593)
                     /locus_tag="PKI33_13085"
     CDS             complement(162..593)
                     /locus_tag="PKI33_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM37996.1"
                     /translation="MFYGFAVLFFVLSLATGFPALLRLSKMEKIRKNGESTFGAVRAA
                     ESDLGWLMTGAMGRVTHPLIEFQVNEKKHTIQVSDNRGFLAPRYDAGDTLEVIYDKDT
                     PWKAYIQQEWRIARADLFKSVAEIILAVVLWETGVSLGMPL"
     gene            709..1284
                     /locus_tag="PKI33_13090"
     CDS             709..1284
                     /locus_tag="PKI33_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNM37997.1"
                     /translation="MPNSTELPILPFESKKKFSEWLAKNHDKSAGLWLKIAKKAAGIS
                     TVTYAEALDVALCYGWIDGQKGSFDEQYFLQKFTPRRAKSIWSKINVEKVAGLIASGE
                     MKPSGLKAVEAAKQDGRWDAAYSSQKNIVVPADFQSALEKNKKAKAFFETLTGAKRYS
                     FLFRIETAKKAETREKRIGQFVQMLAKGETF"
     gene            complement(1294..>1884)
                     /locus_tag="PKI33_13095"
     CDS             complement(1294..>1884)
                     /locus_tag="PKI33_13095"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNM37998.1"
                     /translation="AGMIALWALRLGTFLFTRVRAAGEDRRFREIKPNFARFMLTWTL
                     QGLWVSFSMATALVVMTSTTPVGMDIFMVVGFVVWLFGFVLEVTADRQKRAFNANPAN
                     KGKFIRSGLWAWSRHPNYFGEIVLWIGVTIIALPVLRGWQWVTLISPLFITLLLTRLS
                     GVPLLEKRADNEWGGQAEYEAYKAKTPVLIPRAPKP"
BASE COUNT          481 a          459 c          488 g          456 t
ORIGIN      
        1 tagaccagcc agattccggc ttcggttttg taatgttgct tgagccactc gcgccagtct
       61 ttggggttgg tcacatagag tgtgtttgag atttccatag aggtgtcctc ttatacttgt
      121 ttgaaagtat aacggcaaaa gacatcctct ttcggcgtac tctataaagg catccccaaa
      181 gacacccctg tttcccaaag cacaacagcc aggatgatct ctgcgactga tttgaacaag
      241 tccgctcggg cgatgcgcca ttcttgttgg atatatgcct tccagggcgt gtctttgtcg
      301 tagatgactt cgagcgtatc gcctgcatca tagcgcggcg ctaagaatcc gcggttgtcg
      361 ctcacttgga tggtgtgttt cttctcattg acctgaaact cgatcagcgg atgcgtcact
      421 ctgcccatgg cgcccgtcat cagccagccg agatcacttt ctgcggcgcg gacagcccca
      481 aaggtgcttt caccattttt gcgaattttc tccattttgg agaggcgtaa gagggcgggg
      541 aacccggtcg ccaagctgag gacgaaaaat aaaacagcga agccatagaa catgatcaat
      601 ctcccatcta tttatttcca taacttttcg tattttacta tttttttatt ccttataaaa
      661 gtcaccacag cggatttagc agatgaactt gtaatgtaaa atatctccat gcccaactca
      721 actgaacttc ccatccttcc atttgaatcc aaaaagaaat tttcagagtg gcttgcgaag
      781 aatcatgaca agtctgctgg cttgtggttg aagatcgcca agaaagcggc tgggatttcc
      841 acggtcacgt atgccgaggc gttggatgtc gctttgtgct atggctggat cgatggacag
      901 aagggtagtt ttgacgagca atattttcta caaaaattca cgccgcgcag agcgaagagc
      961 atttggtcca agatcaatgt ggagaaggtg gctggtctga tcgcgagtgg ggagatgaag
     1021 ccctcagggt tgaaagcggt cgaagcggcg aaacaggatg ggcgttggga tgcggcgtat
     1081 tcatctcaga agaatattgt ggtgcccgca gattttcagt ccgcgttgga gaagaacaag
     1141 aaagccaaag cctttttcga gaccttgacg ggcgcgaaac gctattcgtt cttgttccga
     1201 attgaaacgg cgaagaaagc cgaaacacgc gagaagcgca tcggtcaatt cgtgcagatg
     1261 ctggcaaaag gggagacgtt ttaactgcaa gccttatggc ttgggcgctc gcggaatcaa
     1321 caccggagtt ttggctttat aggcttcgta ctctgcttgc ccgccccatt cgttgtcggc
     1381 gcgtttttca agcaagggca cgccgcttaa gcgggtgagt agaagggtga taaagagcgg
     1441 ggaaataagc gtgacccatt gccaaccgcg caacacgggc agcgcaatga tggtaacgcc
     1501 aatccacaat acgatctcac caaaataatt cgggtgacgt gaccatgccc ataacccgct
     1561 gcgaatgaac ttgcctttgt tggcagggtt ggcattgaaa gcgcgcttct gtcggtcggc
     1621 agtcacttca agcacaaagc caaataacca caccacaaaa ccgacgacca taaaaatgtc
     1681 catgcctact ggtgtggtgg atgtcattac caccaatgcc gttgccattg aaaaagaaac
     1741 ccataagcct tgcagggtcc aggtcaacat gaagcgtgca aagttcggct tgatctcacg
     1801 aaagcgacgg tcttctccgg cggcgcgaac ccttgtgaat aagaaggtcc ccaaccgcaa
     1861 tgcccagaga gcgatcattc cggc
//
LOCUS       DAOREF010000691         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51470_length_1881_cov_2.714677, whole genome shotgun sequence.
ACCESSION   DAOREF010000691 DAOREF010000000
VERSION     DAOREF010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51470_length_1881_cov_2.714677"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PKI33_13100"
     CDS             complement(<1..1342)
                     /locus_tag="PKI33_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNM37999.1"
                     /translation="MSLLRRIEQGQSSNNQQSSSASNQNSSKQAESETSSSGNSDSSR
                     LSSLQARRVSAPITSPQAGSYFDLKTRVQNKLLAEIDPSMDVTRTDEVRRTIQNLFEQ
                     ILTEENIVLSRPERARLFEQISAEILGFGPLQSLLEDDTITEIMVNGPKNVYIERKGK
                     VHRVPITFESNDHVMRIIDRIVAPLGRRIDESSPYVDARLPDGSRVNAVIPPISLVGP
                     VLTIRKFSKNPITVDQMIQFGSVSAEAVQFLKACVESRLNIIISGGTGSGKTTLLNVL
                     SSFIPSDERILTIENAAELQLRQEHVVTLESRPANIEGRGEITIRDLVINALRMRPER
                     IIVGECRGGETLDMLQAMNTGHDGSMTTAHANTPRDALSRIETMCLMAGMDLPIRAIR
                     EQVASAVDLICQQERMRDGTRKVTTITEVSGMEGDVITMTDIFVFEQTGTENGRI"
     gene            complement(1352..>1881)
                     /locus_tag="PKI33_13105"
     CDS             complement(1352..>1881)
                     /locus_tag="PKI33_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38000.1"
                     /translation="SGIRILAAPQRPEMAEKISSEQFTKVLQFMQKNYSYVIVDTSSI
                     LTDVILSAIDLSDIVVLVATQEIPSIKNSRLILDLLNSMGINKGRIVFAMNRFDKRIA
                     ITPERISENLKHEISAVVPLDEKIAISAVNRGVPFMLDNKSQPISRGVLALAESVRSR
                     IIALDSETEALPAKR"
BASE COUNT          586 a          408 c          333 g          554 t
ORIGIN      
        1 tgattcttcc attttctgtg cctgtttgtt caaacacaaa aatatcagtc attgtgatca
       61 catcaccttc catgccacta acttcggtta ttgtagttac tttacgagtg ccatcacgca
      121 tacgttcttg ttggcatatt aaatccacag cactagcaac ttgttctcga attgccctaa
      181 ttggcaaatc cataccagcc atcaaacaca ttgtttcaat acgagataat gcatcacgag
      241 gagtattagc gtgagcagta gtcattgagc catcatgacc tgtgttcatt gcctgtaaca
      301 tatctaaagt ctctccccca cggcattcgc caacaataat acgctcaggg cgcatacgaa
      361 gtgcattgat aacaagatct cgaatcgtaa tctcacctcg tccttcaata ttagcaggcc
      421 tagactcaag agtaactaca tgctcttgcc gtagctgaag ttccgccgca ttttcaattg
      481 taagaatacg ttcatccgaa ggaataaaac tagatagcac atttaacagc gttgtctttc
      541 cagaacctgt acctccagaa ataataatat tgagacgcga ctctacgcaa gctttcaaga
      601 attgaactgc ttcagcacta actgacccaa attgaatcat ttgatcaacc gtaattgggt
      661 ttttagaaaa tttacgaata gtcaaaacag gtccaaccaa agatattggt ggaataacag
      721 catttacacg ggatccatct ggaagacgag catcaacata agggcttgac tcgtcaatgc
      781 ggcgtcctaa aggagcaacg atacgatcta ttattctcat tacatggtca ttgctttcga
      841 atgtaatagg aacacgatgc accttaccct tacgctcaat atatacattt ttgggtccat
      901 taaccataat ttctgtaata gtgtcatctt caagcagtga ctgtaaagga ccaaacccta
      961 gtatttctgc agaaatttgt tcaaagagtc tagcacgttc tgggcgagat agaacaatgt
     1021 tttcctcagt taatatctgc tcaaaaagat tttgaatagt acgtcgaact tcatccgtcc
     1081 gagtaacatc catagaaggg tctatttcag ctagtaactt attctgaact ctagtcttaa
     1141 ggtcaaaata tgagccagct tgcggagaag ttattggagc actaactcta cgagcctgca
     1201 atgaagataa tcgggaagaa tctgaatttc cgctagacga agtttcgctt tccgcttgtt
     1261 ttgaggaatt ctgatttgat gcactagaag attgttgatt attggaactt tgaccttgct
     1321 caatacgcct tagtaaagac ataaaaactc cttatctttt agcagggagc gcttcagttt
     1381 cactatctaa agcaataata cgagaccgca cagactctgc aagcgccaac acccctctac
     1441 taattggctg cgatttgtta tctaacataa atggaacacc gcgattaaca gcagatatag
     1501 caattttttc atctaatgga acaaccgctg aaatttcatg ctttagattt tcgctaattc
     1561 tctcaggcgt aattgcaata cgtttatcaa agcgattcat agcaaacaca atgcgtcctt
     1621 tattaatccc catactgttt aataaatcaa gaattaatct agaattctta atagatggaa
     1681 tttcttgtgt agcaaccaac accacaatat cacttaaatc aatagcggaa agtataacat
     1741 ccgtaagaat ggaagatgta tctacgatca catatgaata atttttttgc ataaactgca
     1801 aaacttttgt gaactgttca gaagagattt tctcagccat ctccggacgt tggggtgccg
     1861 ctaaaattcg tattccagat g
//
LOCUS       DAOREF010000692         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51701_length_1876_cov_2.453597, whole genome shotgun sequence.
ACCESSION   DAOREF010000692 DAOREF010000000
VERSION     DAOREF010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51701_length_1876_cov_2.453597"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PKI33_13110"
     CDS             <1..1336
                     /locus_tag="PKI33_13110"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNM38001.1"
                     /translation="RALNLDATLIYLGSQLQSFRDGEVPLVIQETTPLLLDVTPQADV
                     ARAMLSQSFTMTLPNVGSGDPGPWTFDVPVLANMLVFNKVDAGGVPQLQVGLDTNALH
                     KSLSELKVVVDREPQDARFIFNESKGQLEAISASTIGRAVDVEASILAINGALQRGEH
                     SVNLVVTEKQPAVTDQATGADLGIVQLVATQTTYFYGSSEARIQNIVTAAAQYDGLLV
                     APGEVFSMGSVLGDVSLENGYAEALIIYGGRTIKGVGGGVCQVSTTLFRTVFFAGYPV
                     VERYSHAYRVPYYEMDQSGSVDGNLVGMDATVYFPLVDFKFKNDTPYWMLMETNVNVA
                     ARTITWKIYSTSDGRSVTWETTGAQNTVPAPETVFEENVDLKTNELKQVDYAAEGADV
                     TVTRTVWRNGAVYFTDQIQTHYEPWAAVCQYGPGTEDPRRQARKSEVCGFSS"
     gene            1391..1609
                     /locus_tag="PKI33_13115"
     CDS             1391..1609
                     /locus_tag="PKI33_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38002.1"
                     /translation="MVSTELLEILRCPSCVREKDGLLKLHKDAWLICQDCGRKYPIVD
                     DIPVMLIEEGDKWVNTAQDALPVPPPAK"
     gene            1616..>1876
                     /locus_tag="PKI33_13120"
     CDS             1616..>1876
                     /locus_tag="PKI33_13120"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNM38003.1"
                     /translation="MKDLFAELTSGDDERAEQAIPAIVDAGAAAIPALLELTHSENAD
                     SRWWAVRALAASPHTQTSDLIPALSDSNPEVRAAAALALSQHP"
BASE COUNT          483 a          453 c          481 g          459 t
ORIGIN      
        1 tcgtgcgtta aatcttgatg caactttgat ttaccttggc tcgcaattac aaagttttcg
       61 agatggggaa gtgccgctcg tgatccaaga aactacccca cttttgttgg atgttacgcc
      121 tcaagcagat gtggcgcgtg ccatgcttag ccagtcattt acgatgacat taccaaatgt
      181 cggttctggt gaccccggtc catggacttt tgacgtgcct gtgttggcaa acatgttggt
      241 cttcaacaaa gtggatgcag gcggtgtgcc gcaattacaa gttggtttgg atacgaacgc
      301 tttacacaaa tcgttgagtg aactgaaggt cgttgtagac cgtgagcctc aagatgcacg
      361 ctttatcttc aacgagtcaa agggacaact tgaagcgatc tctgcttcca cgattggacg
      421 tgctgtggac gtggaggcta gtattctggc aattaacggt gcgttacaac gcggagaaca
      481 cagcgttaat ttggttgtta cagaaaaaca gcctgcagtg acagaccaag ccactggcgc
      541 agaccttggc attgttcagc tcgttgccac ccaaaccact tatttttacg ggtccagcga
      601 agcgcgcatt caaaatattg tcaccgcagc tgcgcagtat gatggtctgc tcgttgctcc
      661 tggcgaagtg ttttccatgg gcagtgtgct tggcgatgtc agcttggaaa atggttatgc
      721 ggaagcactc attatctatg gcggtcgtac catcaaaggc gtgggaggcg gtgtgtgtca
      781 ggtgagcacc accttgttcc gcaccgtgtt ctttgcaggg tacccagtgg tggaacgtta
      841 ttctcacgcc tatcgtgtgc cttattatga aatggatcaa agcggctcag tggacggaaa
      901 cttggttggc atggacgcca cggtttattt ccccttggtc gactttaagt tcaaaaacga
      961 tacgccttac tggatgttga tggaaaccaa tgttaatgta gctgcgcgca ccatcacgtg
     1021 gaaaatctat tccacatcag atggacgttc ggtcacttgg gaaacaacag gagcacaaaa
     1081 tacggtccct gccccagaaa cggtctttga agaaaacgta gatttaaaga caaacgaact
     1141 taaacaagta gattacgctg ctgaaggtgc tgatgtaacc gtcacacgca cggtatggcg
     1201 taatggggca gtatatttca ccgaccaaat tcaaactcac tatgagccgt gggctgcggt
     1261 ttgtcaatat ggtcccggca cagaagatcc gcgaagacaa gcaaggaaga gcgaagtttg
     1321 tggctttagt tcataaataa aatcttgtca aaacctacat ggtacaatct tttcccaaca
     1381 aggagaaaca atggtcagta ctgaattact cgaaatcttg cgctgcccca gctgcgtgcg
     1441 cgaaaaagac ggtttgctga aactccacaa agatgcctgg ctcatctgcc aggattgcgg
     1501 acgaaaatat cccattgtgg atgacatccc cgtgatgttg attgaagaag gcgataaatg
     1561 ggtgaacacc gcgcaagatg cgttgcccgt cccgccgcct gcaaaataaa taaaaatgaa
     1621 agacctgttc gctgagctta caagcggaga cgatgaacgc gcagaacaag ccattcccgc
     1681 tattgtagat gcaggagcgg cggcaattcc ggccttactg gaattaaccc actctgaaaa
     1741 tgcagactca cgttggtggg ctgtgcgcgc cctcgccgct tcgcctcaca cccagacctc
     1801 agacttgatc cccgccctaa gcgactccaa tcctgaggtg cgagctgctg ctgcgttggc
     1861 attgtcccag caccct
//
LOCUS       DAOREF010000693         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_51961_length_1871_cov_4.411894, whole genome shotgun sequence.
ACCESSION   DAOREF010000693 DAOREF010000000
VERSION     DAOREF010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51961_length_1871_cov_4.411894"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKI33_13125"
     CDS             complement(<1..476)
                     /locus_tag="PKI33_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38004.1"
                     /translation="MKTRNFLIRIGLALLVAALLGAGAGLLAREKVDAAEGTYKILAW
                     NDLGMHCYNRDFADLAVLPPYNTLWVQVIKAGDPPTLVTSGVTVEYFYEDNTYSVGKT
                     NFWSYSQDLFGVNLPPNVGLKGKGLSGTMDFSGDHFVAEGIPVTEFSDSAPTTSQP"
     gene            715..1170
                     /locus_tag="PKI33_13130"
     CDS             715..1170
                     /locus_tag="PKI33_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38005.1"
                     /translation="MENIGGLAAEIVVAFVIALATGAFTFMGFWRQAKADLEQEYLKR
                     FNDKKWGVYTEFAKLLQAPSLNTFETDHRQEAETAIASQIVLIGSDEVVRAFRDWRES
                     VAVHGKAQRVTNEKLFALVANMRRDLGIKYSNLEVDDLLGVLNPSMGDG"
     gene            1176..>1871
                     /locus_tag="PKI33_13135"
     CDS             1176..>1871
                     /locus_tag="PKI33_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38006.1"
                     /translation="MPADTRISRQARLSILGTAVLVISLSGLSFFIEIKLVWQLVISW
                     GYVYILRAADAVYVSPEASLASSMLFANISVFVLIFFVVLFWVSPFILPVRGLVQTQK
                     AFTGLIRSLAGKKWHGPAIFVKEGKPNMEEHELDSHKAGVALVDLYSAIVLEQQTGSI
                     DSLRQEVHFEAEEEDVQGLQRPGMWKQITAKLGVAGKNVNVTFVKAFGPGVVFTESGE
                     KIVGFVDLRKQSRS"
BASE COUNT          439 a          379 c          511 g          542 t
ORIGIN      
        1 tagggttggc tggtggtagg ggcgctgtca ctgaattctg taacagggat accttctgct
       61 acaaaatggt cgccgctgaa atccatggtc ccagaaagac cttttccttt cagaccgaca
      121 ttaggcggta ggttaacccc gaacaggtct tgcgagtagg accagaaatt ggtcttcccg
      181 accgagtagg tgttgtcttc gtagaaatat tccacggtaa caccgctggt gaccagagtc
      241 ggcggatcgc cagctttgat cacctgaacc cataaggtgt tgtatggggg taatactgcg
      301 aggtctgcaa agtcacggtt atagcagtgc attcccaggt cattccaagc caggattttg
      361 tatgtcccct ctgcggcatc caccttttca cgtgctagta gacctgcccc tgctcccaat
      421 agggcagcaa ctaatagtgc cagaccgatg cgaatcaaga aatttcttgt tttcatgctt
      481 gtctccttta tgttggcgaa atacaggtat cttatatata gtcgctggtg tgcggaaaaa
      541 gttacggact gtccataatt atgacacact tcgcctattt ggagttgagt tttgtgagat
      601 ggtgaattgc atccaaaaat cccccctctt gacaatgaag acgttgcgtg tatatactga
      661 tttttgagca tcgtatctat ccacattttc aatcagttat cggggagtct tcatatggaa
      721 aacatcggtg ggttagcggc agagatcgtg gttgcgttcg taattgccct ggcaacaggt
      781 gcatttactt tcatggggtt ttggcgacag gcaaaagcag atctcgaaca ggaatatttg
      841 aaacgattta acgacaagaa gtggggtgtc tatacggagt ttgcaaaact gctgcaagcc
      901 ccttctctta atacctttga aacggatcac aggcaggagg cggaaacagc cattgcctca
      961 cagatcgttt tgatcggttc ggatgaagtg gtccgtgctt ttcgtgattg gcgtgaaagt
     1021 gtggctgtac atgggaaagc ccaacgcgta accaacgaga agctgtttgc tcttgttgcc
     1081 aatatgcgac gcgatctggg gatcaaatat agcaacctgg aggttgatga tttattggga
     1141 gtcttgaatc cctcaatggg ggacggatga atattatgcc cgcggatacc cggatctcac
     1201 ggcaggcgag gctttcgatt cttggaactg ccgttttggt catatctctg tctggtttgt
     1261 ctttcttcat cgaaataaaa cttgtctggc agttggtcat ttcatgggga tatgtttata
     1321 ttttgcgcgc agctgatgcg gtgtatgttt ctccagaggc tagcctcgcc agttcgatgc
     1381 tttttgccaa tatttccgtt tttgttctca tattttttgt tgtgctgttt tgggtatctc
     1441 cgtttatttt gcctgtacgc ggtttggtgc aaactcaaaa agcgtttacc gggttgatcc
     1501 gttctttggc tgggaaaaaa tggcatggac cggcgatctt tgtaaaagaa ggaaagccga
     1561 acatggagga acatgaatta gacagccata aggctggtgt ggctttggtg gatctgtata
     1621 gtgcgatcgt tttggaacaa cagactgggt ccattgattc gttgcggcag gaggtgcatt
     1681 ttgaagctga ggaggaggat gttcaagggc tgcaacggcc tgggatgtgg aagcagatta
     1741 cggctaagtt gggagtcgct gggaagaatg tcaatgtcac ttttgtgaag gcattcggtc
     1801 ccggtgtcgt ctttaccgaa tccggggaaa agatcgtggg gtttgtggat ctgagaaagc
     1861 agagtagatc t
//
LOCUS       DAOREF010000694         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_52059_length_1869_cov_4.638368, whole genome shotgun sequence.
ACCESSION   DAOREF010000694 DAOREF010000000
VERSION     DAOREF010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52059_length_1869_cov_4.638368"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PKI33_13140"
     CDS             complement(<1..1501)
                     /locus_tag="PKI33_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNM38007.1"
                     /translation="METLVTPGFDPAHDVLHYGRQPLDSIFAPKSVAVVGATETVGSV
                     GRTIVWNLMSSSFGGTIYPVNPKRPSVLGIKAYPDLKSIPEKLDLIVVVTPAPTVPDI
                     IKEAVDLGVPSAIIISAGFKETGPAGVELERQVMEHARRGNMRIIGPNCLGVMSPTTG
                     LNATFASTIARRGSVGFISQSGALCTAILDWSLTENVGFSSFVSIGSMLDVDWSDLIY
                     YLGDDPHTKSIVIYMETIGNARAFLSAAREVAMTKPIIVIKPGRTEGAAKAAASHTGS
                     LTGSDEVLEVAFRRSGVLRVNSIAELFYMAEVLGKQPRPKGPRLTIVTNAGGPGVLAT
                     DALISNGGELTTISPETMEEFNKLLPAAWSHNNPVDILGDASPERYAKALEIAAKDEN
                     SDGILVILTPQAMTDPTETADVLKPYAQGLGKPVLATWMGGKEVAPGEAILNKVNIPT
                     FPYPDTAARVFDYMAHYSENLHSLYETPMSMDDGGDAIDRERAAKIIEDV"
BASE COUNT          439 a          458 c          501 g          471 t
ORIGIN      
        1 gaacatcctc aatgattttt gcggcgcgtt cacggtcaat cgcatcgccg ccatcatcca
       61 tagacatggg cgtttcatac aagctatgca agttctcgga ataatgcgcc atgtaatcga
      121 agacgcgcgc cgccgtatcg ggataaggga aggtggggat gttcactttg ttgagaatgg
      181 cttcacccgg tgccacttct ttgccgccca tccaagtagc gaggacgggt tttcccaagc
      241 cttgtgcgta tggcttcaag acgtctgcag tttcggtagg gtcggtcatg gcttgcgggg
      301 tgaggatgac taaaatgccg tcgctgtttt cgtctttggc tgcgatctca agtgctttgg
      361 cgtaacgttc agggctggca tcgccaagga tatcgaccgg gttgttatgg ctccacgcgg
      421 cgggaagcag tttgttgaat tcttccatcg tctctggtga gatagtggta agttcaccgc
      481 cattcgaaat gagtgcatcg gtggcgagca cgccgggtcc gccagcattg gtaacaatcg
      541 tcaggcgcgg acctttcggg cgcggctgtt tgccgagcac ttctgccatg tagaacaatt
      601 cggcaatgct atttacgcgc aacacaccac tgcgacggaa agcaacttca agcacttcgt
      661 cactaccggt caatgaaccg gtgtgagaag ccgctgcttt ggctgcacct tcggtgcgcc
      721 ccggtttgat gacgatgatg ggtttggtca tggcaacttc acgcgccgca gaaaggaaag
      781 cacgtgcatt accaatcgtt tccatataaa tgacgatgct cttggtgtgt gggtcatcgc
      841 cgagatagta aataaggtcg ctccagtcca catcgagcat ggagccgatg gagacgaagg
      901 aactaaagcc cacgttttcc gtgaggctcc aatccaagat agctgtacaa agcgcgccac
      961 tctggctgat gaagcccacg cttccacgtc gtgcaatggt gctggcgaag gtcgcgttca
     1021 agccagtagt tgggctcatt acaccgaggc agttcggtcc gatgatgcgc atgtttccac
     1081 ggcgggcatg ttccatcacc tggcgctcaa gttccacccc tgccggaccc gtctctttga
     1141 atccagccga aatgatgatg gcgcttggca caccaagatc cacggcttct ttgatgatat
     1201 cgggcacagt cggcgccggt gtgacgacaa cgataaggtc taatttttca ggaatagatt
     1261 tcaaatctgg gtaggctttg atgccaagga cgcttgggcg cttcgggttg acggggtaaa
     1321 tggtgccgcc aaaggagctg ctcattaagt tccaaacaat ggtccgcccc acgctgccga
     1381 cggtttcagt agcaccgaca acggcaacgc tttttggggc aaagatagag tctaggggtt
     1441 ggcgcccata atgtagaaca tcatgagcgg ggtcgaatcc gggagtgaca agggtttcca
     1501 taatttactt ctttctttaa agggtacgtt gttacgatgt tttttcatcg taacaatcag
     1561 ttttgaagac aggatgatac aaagacatat ataccactag aagcgggtgc tcaatttcac
     1621 gtttcttata gattaacttc cttattcttg attttttccc aacaaaaaag cggatgtctt
     1681 cctaaaaaga catccgcttt ctaacgattc cgtcagtttc ttgcaagttc catcaaaaaa
     1741 acatcaggag ccaaacacca caaaatccaa cgggtcgagt tccagacttt tctttgcaga
     1801 gacatggtta tgcccatgca gttttttcaa gctggtgatg gagtattgat caatgatgcg
     1861 cggagggag
//
LOCUS       DAOREF010000695         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_52063_length_1869_cov_3.780595, whole genome shotgun sequence.
ACCESSION   DAOREF010000695 DAOREF010000000
VERSION     DAOREF010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52063_length_1869_cov_3.780595"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKI33_13145"
     CDS             <1..668
                     /locus_tag="PKI33_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38008.1"
                     /translation="QLYGYDAWQYATSQTGVVVTYVVLFENPFNYAIEPLIPATLTQP
                     QMSLPFAAGESWYFTGGPHGAWDSGSAWGALDFAPPDNSGGCNISSSWVTAIADGLVT
                     RTGTGSVYQDLDNDGYEQTGWVIIYMHVASAGRVTPGEYLFRNEKVGHPSCEGGISNA
                     THVHITRKYNGEWIAADGPYPWNLDGWISSGTGYEYDGFLTRNGVSIEALDAAQPANL
                     ISR"
     gene            710..>1869
                     /locus_tag="PKI33_13150"
     CDS             710..>1869
                     /locus_tag="PKI33_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM38009.1"
                     /translation="MQRTFAVFIALSMTISACGASPSIWGVVPTPTPNASQTAPLTDP
                     FAGQSDPIVFPTSTIPPFIETENAQTPTPQVTEETVTTQVEPSITTAPLNDAAPFLYY
                     AQSGDMLSAVAARFGVSESEIISDADLTKTTLIDPGTLLVIPNRINEPTTPNVQLLPD
                     AEFVFSATSIGFDTEKFVKDQNGYLSSFRDYLGSVGWVQGYDAIDRLSVENSVSPRLL
                     LALLEYEARWVRGQPIDLLHTEFPMGFNDYHYKGMSVQMTWAINNMSIAYYGWRAGTI
                     THIEFPDGTRLRLDPRLNAGTVAIQYLFSKLHSESQWSQIINPDSGFPALYNEMFGDP
                     WARADLVGPIFPPGLIQPPLVLPFEPGAKWSFTGGPHNGWGQISPSIYGQSH"
BASE COUNT          441 a          522 c          417 g          489 t
ORIGIN      
        1 cacagcttta tggttatgat gcctggcaat acgctacaag tcaaactggc gtggtggtca
       61 catacgttgt cctttttgaa aatccattca attatgccat tgaaccatta atccctgcca
      121 cactcacaca gccgcaaatg tctctgccgt ttgcagctgg agaaagttgg tactttacgg
      181 gtggtccaca cggtgcctgg gactccggtt ctgcttgggg cgcgctcgat tttgccccgc
      241 cagataacag tggtggttgt aacatcagct ccagttgggt caccgctatt gcagatggtc
      301 tcgtcactcg tacgggtacc ggctcggtat atcaagacct tgataatgat ggctacgaac
      361 aaaccggctg ggtcattatt tacatgcatg tcgcatccgc tgggcgtgtt acacccggcg
      421 aatatctttt ccgcaatgaa aaagtgggac acccttcttg tgaaggcggc atctcgaacg
      481 ctacgcatgt ccatatcaca cgcaaataca acggcgaatg gattgccgca gatggtccct
      541 acccttggaa ccttgatggt tggatatcca gcggcaccgg ctatgaatat gatggtttcc
      601 tcactcgtaa cggcgttagt attgaagcct tagatgcggc tcaaccagcc aacctcattt
      661 cacgctaact cacttgatat taacctaact ggttataatc actcaaacca tgcaacgtac
      721 ctttgccgtt ttcatcgcac tatcgatgac catttctgcc tgtggggctt cgccttccat
      781 ttggggagtt gttcccaccc caaccccaaa cgcctctcaa acagcgcctc ttacggaccc
      841 gttcgctggt cagagtgacc cgatcgtttt ccccacctca accattccgc ctttcattga
      901 gacggagaat gcacaaacgc caacaccaca agttactgaa gaaactgtta caacccaagt
      961 ggaaccatcc attaccacgg ctccgcttaa tgatgccgca ccttttctat actatgccca
     1021 aagcggagat atgctctctg ccgttgcagc tcgctttggt gtgagcgaat ctgagattat
     1081 ttcagatgca gatctcacca aaacaaccct aatcgacccc ggcaccctgc ttgtcattcc
     1141 aaaccgcatc aacgaaccca caacgcccaa tgttcagctt ttgccagatg ctgaatttgt
     1201 tttctctgcc acgtccatag gctttgatac agaaaaattt gttaaagacc aaaacgggta
     1261 tctcagttcg tttcgtgatt acttagggtc tgtcggttgg gtacagggtt acgacgccat
     1321 tgatcgtctt tccgtcgaga actctgtcag cccgcgcttg ttgctggctt tgcttgaata
     1381 cgaagcgcgc tgggtacgcg gtcagcccat agacttgtta cacactgaat tcccaatggg
     1441 ttttaacgac tatcattaca aaggcatgag tgtacagatg acatgggcga tcaacaacat
     1501 gtcgattgcc tattacggtt ggcgcgctgg caccataacg catattgaat tccccgacgg
     1561 tacaagactc cgccttgacc ctcgtctcaa tgcaggcacc gttgccattc aatatttatt
     1621 ttcaaagctt cacagtgaat cccagtggtc acaaattatc aatccggact ctggcttccc
     1681 agccttgtac aacgaaatgt tcggtgaccc ttgggcacgc gcagatttag tcggtcccat
     1741 tttccctccc ggcttaattc aaccaccgct tgtcctgccg tttgaaccgg gtgccaaatg
     1801 gagcttcacg ggcggtccgc acaacggctg ggggcagatc agcccaagta tttatgggca
     1861 gtctcatag
//
LOCUS       DAOREF010000696         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_52378_length_1863_cov_5.293695, whole genome shotgun sequence.
ACCESSION   DAOREF010000696 DAOREF010000000
VERSION     DAOREF010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52378_length_1863_cov_5.293695"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKI33_13155"
     CDS             complement(<1..619)
                     /locus_tag="PKI33_13155"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNM38010.1"
                     /translation="MNTSLPLLELMRGPVVEATHLGSIAVVTADGTLLHSYGDPHTVA
                     FLRSSAKPFQVLPFVEAGGVEHYNYTQRELALSCASHETSQMHLDAVTALQKKVGIEE
                     HDLQCGPHLPGDARKLREVIQQNILPTANFNNCSGKHTSMLAFAKMRGLPLTNYLAID
                     HPIQQDILKTLSEMCNIAVNKIQLGVDGCSAPNFALPLFHAALGMA"
     gene            complement(619..1476)
                     /locus_tag="PKI33_13160"
     CDS             complement(619..1476)
                     /locus_tag="PKI33_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM38011.1"
                     /translation="MQEITKNIFIEDQFLGVTLGVIVTPRGLIQIDAPPSPEDARSWR
                     ASLMNLGGGIDRLLINMDAHPDRTLGARAMDCTVVAHEKTAYIFRTRPSTFKAQGEET
                     GADWESIPGLGSVRWAPPEISFVDQMTLHWGSSPVMLEHRPGPSGGSIWVNLPEEKVL
                     FVGDMVMKNQPPFLAGSNLKAWLESLDLLLEPEYKGYTFISSRGGVVNSSAIKAQYDF
                     LKHVHDKLNKSTSKKPNPAAVEKMVTSLLTWFKAPAARQKQFAQRLRYGLLHYNARQS
                     YAPSNTIED"
     gene            complement(1536..>1863)
                     /locus_tag="PKI33_13165"
     CDS             complement(1536..>1863)
                     /locus_tag="PKI33_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNM38012.1"
                     /translation="IDIGAQTNEDGLFVRFWIRDNGPGIPAAERERIFEKFTRLRGKN
                     KAGGLGVGLAFCRLAVHAHDGDIWIESEEGKGTTFWLTLPVAQRKSAGQIKRQTGRLT
                     FKPDKP"
BASE COUNT          446 a          423 c          518 g          476 t
ORIGIN      
        1 gagccatacc gagggcagca tggaacaatg gcagtgcaaa gtttggcgca gagcagccat
       61 caacaccgag ttgaatttta ttgacggcaa tgttgcacat ttctgaaagt gtcttcaaaa
      121 tatcttgttg aatggggtgg tcgatagcaa gatagtttgt gaggggaagt ccgcgcattt
      181 tggcgaatgc taacatggaa gtgtgcttgc ccgaacaatt gttgaagttt gcagtgggga
      241 ggatgttctg ttgaatgact tcgcgtaatt ttcgagcatc acccggcagg tgtggaccgc
      301 actgtaaatc atgctcttca atgccaactt ttttctgtaa agcggtcaca gcatcgagat
      361 gcatttgtga ggtctcgtgt gaagcgcagg agagagccaa ctcacgttgg gtgtagttgt
      421 aatgctccac gccgccagct tcaacgaaag gcagcacttg gaatggtttg gcagaggagc
      481 gcaaaaaggc gaccgtgtga gggtcgccat aagagtgaag caacgttcca tctgctgtga
      541 ccactgcgat ggagccaagg tgggtggctt caaccacagg tccgcgcatg agttcaagaa
      601 gagggagaga tgtattcatt aatcttctat ggtattgctg ggtgcatagc tctgacgagc
      661 gttgtagtgc aataagccgt aacgcaaacg ttgggcaaat tgtttctgac gagccgctgg
      721 ggctttgaac caagtcaaca acgacgtaac cattttttct acagcggcag ggtttggctt
      781 cttgctggtg gacttattca atttgtcgtg gacatgcttg aggaagtcat attgtgcttt
      841 gatggcactg ctattgacca cgccaccgcg gctgctgatg aaggtatacc ctttatattc
      901 tggttctagc aagaggtcta agctttcgag ccaggctttg aggttagagc cagcaagaaa
      961 aggaggttga tttttcatga ccatatcgcc aacaaagaga actttttctt caggcaagtt
     1021 aacccagatc gagccgccag aaggaccggg gcgatgttct aacatcacag gggagctgcc
     1081 ccagtgcagg gtcatttggt caacaaaaga aatctcggga ggtgcccaac gcacactgcc
     1141 caagccggga atggattccc aatcggcgcc ggtttcttcg ccttgtgctt tgaaagtgct
     1201 gggacgagta cggaaaatgt acgcagtctt ttcatgggct acaacggtgc aatccatggc
     1261 gcgggcgccg agggtgcggt ctggatgggc atccatgttg atcagtaaac ggtcgatgcc
     1321 cccgcctaaa ttcatgagag aggcgcgcca agagcgggca tcttcaggag agggaggcgc
     1381 gtcgatctga attaatccgc gcggggtgac gatcacgccc aaggtgacgc ctaaaaattg
     1441 gtcttcgatg aaaatgttct ttgtgatttc ctgcatgatg gttctgaaaa aaattccttt
     1501 cggatctatt gtccttcgtt gttctttcta tctttttatg gcttatctgg cttgaaggtg
     1561 agtctaccgg tctggcgttt gatctgacca gcagatttgc gttgcgccac gggcagggtg
     1621 agccagaaag tggttccttt gccttcttca ctttcgatcc agatatcacc atcgtgagcg
     1681 tggactgcaa gccgacaaaa agcaagccct acccccaagc cgcctgcttt gttcttccca
     1741 cgcaggcgtg taaacttttc gaaaatgcgt tcacgttccg cagcgggaat gccgggaccg
     1801 ttatctcgta tccaaaatct gacgaacaaa ccgtcttcat tggtctgtgc accaatgtca
     1861 atg
//
LOCUS       DAOREF010000697         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_52433_length_1862_cov_5.130603, whole genome shotgun sequence.
ACCESSION   DAOREF010000697 DAOREF010000000
VERSION     DAOREF010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52433_length_1862_cov_5.130603"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKI33_13170"
     CDS             <1..561
                     /locus_tag="PKI33_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38013.1"
                     /translation="EIRERLRHERNSIAGFTARPSKPPKEWGGSQRGYAPEAELGLEL
                     AGLADIPLMAFGKLEFIAAQHGLNPAKLMKPAPFHALAGILAAWTGDELSALNAAYEW
                     HTSGKLNGKFSELPKSFELYVIEDTMGGVRATKAAGEILRAAGFTVTTHTLGLTSGIA
                     QKAEAFRKADVPFFENWESLIAGMGL"
     gene            635..1336
                     /locus_tag="PKI33_13175"
     CDS             635..1336
                     /locus_tag="PKI33_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNM38014.1"
                     /translation="MNNYIALAITFAIALGFLRLMDFFAHRGWIESKLSRKLIHIGTG
                     PIFVLCWFFFDYAPSARWLAALVPFAITVQFALIGLGVIKDKASVDAMSRTGDPKEIL
                     RGPLYYGIMFVVLTLVYWKDSPIGIIALMMMCGGDGIADIFGRRIPSPKLPWSKEKSV
                     AGMVSVFVGGFVFSAMMLFIYVNAGVFAGPFTNYLLPMAAIAFVGAAVESLHYKDIDN
                     ISMTLASALLGHWFF"
     gene            complement(1388..>1862)
                     /locus_tag="PKI33_13180"
     CDS             complement(1388..>1862)
                     /locus_tag="PKI33_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNM38015.1"
                     /translation="PDGELEPTIALRRELTRLIRMYKPDVVLAGNPEAWFYGNEYVNH
                     PDHRAAAQAACEAVFPSAGSRLIFADLLTEGFEPHEVKRLYVHGTEKPDTWVDVSETI
                     DIKVKALQQHASQVPVDEVEKWMKDWAKEDAKDKEFEYAESYRVMILKKEEEHEK"
BASE COUNT          370 a          455 c          532 g          505 t
ORIGIN      
        1 gagatccgcg agaggttaag gcacgagcgg aattccatcg caggttttac tgccagacct
       61 tccaagccgc ccaaggaatg gggcggttct cagcgaggtt acgcgcccga agcggagttg
      121 gggctggagt tggcagggct ggcggatatt ccgttgatgg catttgggaa gttggagttc
      181 atcgcggcgc agcacggctt gaaccccgcg aaattgatga agcccgctcc gtttcatgcg
      241 ttggcaggaa tacttgcggc atggactggt gatgagttgt cagcgttgaa tgcggcgtat
      301 gagtggcaca catcgggaaa gttgaatggg aagttcagcg agttgccgaa gtcgttcgag
      361 ttgtacgtca tcgaagatac gatgggcggc gtgcgtgcga caaaagcggc gggagagatt
      421 ttgcgtgcgg caggctttac ggtgactaca cacacgctcg gtctgacttc aggcattgcg
      481 caaaaagcgg aggcgttccg caaagcagat gtgccatttt ttgaaaattg ggagtctttg
      541 atagccggta tggggttata aataatgacg atagacgata gaccgtggac aattgatgag
      601 atcgtctatc gccaattcac tcaggagttt ccctttgaac aactacattg cccttgctat
      661 tacttttgcc attgcgcttg gctttttgcg cctgatggat ttcttcgccc atcgcggctg
      721 gatcgaaagc aagctcagcc gcaagttgat ccacatcggc acgggtccga tcttcgtgtt
      781 gtgctggttc tttttcgact atgctccttc cgcccgatgg ctggcagcgc tggttccgtt
      841 cgcgatcacg gtgcagttcg cgctgatcgg tttgggtgtg atcaaagaca aagcctctgt
      901 agatgccatg tctcgtaccg gcgacccgaa ggagattctg cgtggtccgc tgtattacgg
      961 aattatgttc gtggtgttga ccctggtgta ctggaaagat tcccccatcg gcattattgc
     1021 gttgatgatg atgtgcggcg gtgatggtat tgccgatatc tttgggcggc gcatcccttc
     1081 gccaaaattg ccgtggagca aggaaaagtc ggtggcgggg atggtgagcg tgttcgtagg
     1141 cggatttgtc ttttcagcca tgatgctttt tatttacgtg aatgccggtg tgttcgcagg
     1201 tccgttcaca aattatctcc tgcccatggc tgctattgcc tttgttggcg cagcggtgga
     1261 gtcgctgcat tataaggata ttgataatat cagcatgaca ctcgcgtctg cattgttggg
     1321 acattggttc ttttagaata aaagaggcag tcacttcaac cgtgactgcc tctttagttt
     1381 taggggatta tttctcgtgc tcttcttctt tcttcaagat catcaccctg tacgactcgg
     1441 catattcaaa ctctttatcc ttcgcgtctt ccttcgccca atctttcatc cacttttcaa
     1501 cctcatccac tggcacctgg ctcgcatgct gctgtaaggc cttcactttg atatcgatgg
     1561 tctcggagac atccacccag gtatcgggtt tctccgtacc gtggacgtag agccgcttca
     1621 cttcgtgcgg ttcgaacccc tctgtgagca ggtcggcaaa gatcagcctt gaccctgccg
     1681 acgggaagac cgcttcgcaa gcggcttggg cggcggcgcg atgatccgga tggttgacgt
     1741 attcattgcc gtagaaccat gcttcagggt tgcccgccaa aaccacatcc ggtttgtaca
     1801 tccggataag tcttgtaagt tccctgcgca gcgcaatggt cggctccagt tcgccatctg
     1861 gg
//
LOCUS       DAOREF010000698         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_52589_length_1859_cov_5.473947, whole genome shotgun sequence.
ACCESSION   DAOREF010000698 DAOREF010000000
VERSION     DAOREF010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52589_length_1859_cov_5.473947"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1859)
                     /locus_tag="PKI33_13185"
     CDS             complement(<1..>1859)
                     /locus_tag="PKI33_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNM38016.1"
                     /translation="IVGPNGVGKTSLLRILAGEDESSSGSVTRTRGVRTGYLSQEADF
                     KMEGTLWDACHSVFDDLIRQQKELHRLEELMATDPDVIEQYGKMQEEFERRGGYTYEI
                     RMKQVLSGLGFDASDYSLSLDHLSGGQRTRGFLARLLLSNPDVLLLDEPTNHLDIRAV
                     EWLEGYLNQWDGAAIIVSHDRYFLDKVSTEILEMLPGAYEIYKGNYKAYLKQREERIA
                     RRQEVFESEKEKLLKEVDYIKKHIAGQNTLQAKGKLKRLSRLVQAIEQIGFDAALNQK
                     WSETADEVSVTTSSFSAEEAERRVRALRSPVRTLPNLHLRLGSEKRSGDLVIRTKNLS
                     VGFPDKFLFKSPDIELRRQDCAALIGPNGAGKSTFLKTILGQFEPLGGEVELGSSLHI
                     GYFAQAHEGLDPEKTLLQEIDSVMPHWQPGQIRDYLGKYLFSGDDVYKKVGMLSGGER
                     GRLALSKLALQDTNLLLLDEPTNHLDIPSQEVLEAVLEDYTGTILLISHDRYLVDALA
                     TQIWEVNPDAGELIAFKGTYSELRAEREQKEYEAFMAAQKNNDTRTSGSDNRGSTNES
                     RKSKNANTKEERRKIAQLQELENKIAELESTLANLSSQLESPMVKPDEVIKIG"
BASE COUNT          406 a          493 c          454 g          506 t
ORIGIN      
        1 ttgccgatct tgatgacttc atctggcttg accatgggac tctccaactg cgagctcaaa
       61 ttcgccagcg tagactccaa ctcggcgatc ttgttttcca gttcttgaag ctgggcaatc
      121 tttcgtcttt cttccttcgt gttcgcattt ttggattttc gagattcgtt ggtcgatcct
      181 cgattgtcac ttcccgaagt tcgagtgtca ttatttttct gcgccgccat gaacgcttca
      241 tattcctttt gctcgcgttc ggctcttaac tcagaatagg ttcctttgaa tgcgattaac
      301 tcaccggcat cggggttgac ttcccatatt tgggttgcca atgcatcgac gaggtaacgg
      361 tcatgcgaga tgagcaaaat agtcccggtg taatcttcca gtacagcctc taagacttcc
      421 tgtgaaggaa tatcaaggtg gttggtcggt tcatccaata acaagagatt cgtatcttgc
      481 aacgcgagtt tcgacaacgc caaacgtccg cgctcgccgc cggagagcat gcccactttt
      541 ttatacacat cgtcgccgct aaagagatac ttgcccaaat aatcgcggat ctgtcccggc
      601 tgccaatgcg gcataaccga gtcgatctct tgcagcaaag tcttttctgg gtcgagccct
      661 tcatgcgctt gggcaaagta accaatgtgt aaacttgaac ccagttctac ttctccaccc
      721 aaaggctcga actgaccgag aatagtcttc aagaatgtgg atttacctgc gccattcgga
      781 ccgatcaatg cggcgcagtc ttgtcttctc agttcaatgt ctggcgactt gaagaggaat
      841 ttatcaggga agcccacact caagtttttg gtacgaatga ccaagtcacc agagcgcttc
      901 tcagagccga ggcgtagatg caaattaggt agcgttcgta cgggagagcg gagcgcacgc
      961 acacggcgtt ccgcttcttc tgcgctgaac gaagaagtgg tgacactgac ttcgtcggca
     1021 gtctcactcc atttttgatt caatgccgca tcaaaaccga tctgctcaat cgcttgaacc
     1081 aggcgtgaga gtcgtttcag cttgcctttg gcttgcaagg tgttctgccc ggcaatgtgt
     1141 tttttgatgt aatccacttc tttgagcagt ttttcttttt cgctctcgaa cacttcctgt
     1201 ctgcgggcaa tgcgttcttc acgttgtttc agatacgctt tgtaattgcc cttgtatatc
     1261 tcatacgcgc cgggcagcat ttccaaaatt tcggtgctga ccttatccaa aaaataacgg
     1321 tcatgcgaaa cgatgattgc cgcaccatcc cattgattca aatagccttc cagccattcc
     1381 accgcgcgga tgtcgaggtg gttggtgggt tcatcgagca ggagcacatc ggggttcgag
     1441 agcaagaggc gggcgagaaa gcctcgagtg cgctgtccgc ctgaaaggtg atccaaagac
     1501 aggctgtagt ctgaggcgtc gaatcctaaa ccagaaagca cctgcttcat gcgaatctcg
     1561 taggtgtagc cgccgcgtcg ttcaaactcc tcttgcatct tgccgtattg ttcgatgacg
     1621 tctgggtcgg ttgccatcaa ttcttcgagg cggtgcaatt ctttttgctg gcggatcaaa
     1681 tcatcgaaga cggaatgaca cgcgtcccaa agcgtgcctt ccattttgaa atcggcttct
     1741 tgcgacaaat acccggttcg gactccccgt gtgcgggtca ctgaacccga gctggattca
     1801 tcctccccgg caaggatgcg caacaggctg gtcttgccca cgccgttcgg accgacaat
//
LOCUS       DAOREF010000699         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_52833_length_1855_cov_2.436111, whole genome shotgun sequence.
ACCESSION   DAOREF010000699 DAOREF010000000
VERSION     DAOREF010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52833_length_1855_cov_2.436111"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1619
                     /locus_tag="PKI33_13190"
     CDS             195..1619
                     /locus_tag="PKI33_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38017.1"
                     /translation="MKLKAPILFAITTLAFAALACQALSGGNSENPSPVATESSTEST
                     TNTTTDTSQPALAPAVSAKGPGIVCAGLRAGGISCLNENGWQTYTDENSDLASSFIDA
                     GTTCPDGRIAISHYDGVSLFDGNTWENINSSNNYSSVNGIACDLNGNIWVAHYQGVSR
                     YSNNAWNTYPSAELATGEFANDLVYGVAATSTGMVWVATSRSVASFDGTTWTVYQQGS
                     GFTEDVFFNAITVDSLDRPWAGVSDGAYVFENNVWKKVKRDGYDTPQAMSIDARGQIW
                     LATLSNGVLAFNGNSWSGYNTDSKHLSSDHANGIAADTLGRVWVGTTYGLSVFDGSNW
                     QTLRMDNSDLGDNYVKFVVATKDGPTLPELADKAKSGLTGSLNDTSNTPMPNMRVEIC
                     VEPLTSSFDGETPCSDQPFFLRTQTDANGVFTFTDVPAGYYVIVAETGDGSWAQLTDE
                     FGIGSERTLLPAGQPYDIGPLTLQ"
     gene            1670..>1855
                     /locus_tag="PKI33_13195"
     CDS             1670..>1855
                     /locus_tag="PKI33_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38018.1"
                     /translation="MDKEPLRTLRFLLSRLERISADSSVAYRASGVRGSMLRMVEKLE
                     KGRPVPGPAVRRLIEIGY"
BASE COUNT          485 a          450 c          453 g          467 t
ORIGIN      
        1 acgctcaatc acggcaaaga agaattgctt gagatcgctc gatctctgcc acaagttttg
       61 gtcaactatt atgaagaact aaagcctgtg ttgactaagt aattcctcca tttaatgcat
      121 gtggagcgag cgtttttatt ttcatggtaa aaatatcgct cagatttaat gtctttactt
      181 gtggagagag aataatgaaa ctaaaagcac ctattttatt tgcgatcaca acactagctt
      241 ttgcagcttt agcatgtcag gcacttagcg gtgggaattc agagaatccc tcccctgttg
      301 caacggaaag ctcaaccgaa agcacaacaa acaccaccac agatacttca caacctgctc
      361 ttgcccctgc cgtctcagca aagggaccgg gcatcgtctg cgcggggtta cgggctggtg
      421 gcatcagttg ccttaacgaa aacggctggc aaacctatac cgatgagaac tcagaccttg
      481 ccagcagctt tatcgatgca ggaactactt gcccagatgg gcggattgca atttctcact
      541 acgatggcgt tagcttattt gacgggaaca cctgggaaaa tataaattca agcaataact
      601 atagttctgt aaatggcata gcctgtgacc taaatggcaa catttgggta gcgcattatc
      661 aaggcgttag tcgctacagc aacaatgcct ggaatacata cccgtccgct gaactagcga
      721 ctggcgagtt tgccaatgac ctggtatatg gggtagccgc cacctcaact ggcatggtat
      781 gggtggctac atcacgtagt gttgcctcat ttgatggcac tacttggacg gtgtaccaac
      841 aaggcagcgg ttttacagaa gacgtcttct tcaatgccat caccgtcgac tcattagacc
      901 gcccgtgggc gggtgtcagt gatggtgcct atgtatttga aaataatgtc tggaaaaagg
      961 tcaaacgaga tggatatgac acccctcaag ccatgagtat tgacgctcgc ggtcaaatct
     1021 ggttggcaac tttatcaaat ggggttttag ccttcaatgg caatagttgg tctggctata
     1081 ataccgactc taaacatcta tcgagtgacc atgccaacgg tattgctgcc gacactttgg
     1141 gacgtgtctg ggttggaacc acctatgggc tcagcgtttt tgacggaagc aattggcaaa
     1201 ccctgcgcat ggataattca gaccttggcg ataattatgt caaatttgta gttgccacca
     1261 aggatggtcc taccttgcct gagttggcag ataaagccaa atctggtctg accggtagcc
     1321 taaatgacac ttccaatacg cccatgccca acatgcgtgt ggaaatttgc gttgaaccgc
     1381 tcacatcctc ttttgacggt gaaacacctt gctccgatca acccttcttc ctgagaacac
     1441 agactgatgc aaacggtgtg tttacgttca cagacgttcc cgccggatat tatgtcattg
     1501 tcgccgaaac cggagatggc agttgggcac aactgaccga tgaatttgga atcggatccg
     1561 aacgtaccct cttgcccgcc gggcagccat acgatatagg tccactcaca ttgcaataag
     1621 cctcaaaaaa cagtcgccga aaagggggct gtttttttat aatcaagtta tggataaaga
     1681 gccgcttcgc actctccgtt tcttgctctc acgcctcgag cgtatctccg ccgattcatc
     1741 ggtggcgtac cgcgccagcg gcgtgagagg gtcgatgctg cggatggtgg aaaagcttga
     1801 aaagggcaga cccgttccag gtccagcagt gaggcggttg atcgagatcg ggtac
//
LOCUS       DAOREF010000700         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_52980_length_1852_cov_4.134669, whole genome shotgun sequence.
ACCESSION   DAOREF010000700 DAOREF010000000
VERSION     DAOREF010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52980_length_1852_cov_4.134669"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKI33_13200"
     CDS             complement(<1..569)
                     /locus_tag="PKI33_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38019.1"
                     /translation="MKISLLEIKEPKILKASEIKIELTTTPVKYYRHGWQSWSLTAWT
                     DLSPLPMQKPAIFHPLQTDAVYANEKNPHGSWLGAVELEDGKILLLGALATDTHVFLV
                     GNQLEGRSEAGEVEWLIAIGEEKTVFDEYVHQLGLRFGRVDKNHIPRVWCSWYSLYIA
                     IDEKILHESFDGLGNLPFDVLQVDDGWQN"
     gene            702..1208
                     /locus_tag="PKI33_13205"
     CDS             702..1208
                     /locus_tag="PKI33_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNM38020.1"
                     /translation="MYEFDKTDIKIVNLLLEDGRMSASEMSRRMGDISERAVRYRVDR
                     MIEEGVIQISAVVNPEALGFNIKADVWLEVEADAVLEVAKKMASFENVTYVACGIGQN
                     DISIQLVARDTSEIYYFVTEVVRKVPGVRKTTTSIVPIILKDVYQWRVPERIAKELSE
                     NETVTASK"
     gene            1254..>1852
                     /locus_tag="PKI33_13210"
     CDS             1254..>1852
                     /locus_tag="PKI33_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM38021.1"
                     /translation="MFGSKTQALQQRAQKVAPLGVNSNFRFWGDGITPYVEKAKGGYL
                     WDVDGKKYIDYRMAFGPIILGHAYAEVDQKVIEEIQKGILFAMTGELEIAVAEMIVDM
                     SPAIDMVRLACSGTEATMHAIRVARAYTGRDIILKFEGNYHGFHDHTLWSTYAPVEAY
                     GNRRSPIPVPASSGIPKMMRENIITLPFNDFEGFERVMRS"
BASE COUNT          477 a          416 c          465 g          494 t
ORIGIN      
        1 tcgttctgcc aaccatcatc cacttgcagc acatcaaaag gcaggtttcc gagcccatca
       61 aaagactcgt gcaagatttt ctcgtcgatg gctatgtaaa ggctatacca tgagcaccat
      121 acacgtggaa tgtgattctt atctactctg ccaaatcgca accctagttg atggacgtat
      181 tcatcgaaga cggttttctc ttcgcctata gcaataagcc attcgacttc gcccgcttca
      241 cttcgccctt cgagttgatt gcccaccaga aacacatgcg tatctgtagc aagtgcgcca
      301 agcaaaagga ttttgccatc ttcaagttca actgcgccta gccatgaacc atgcggattc
      361 ttttcatttg cataaaccgc gtcggtttgt aatgggtgaa atatggcggg tttttgcatg
      421 gggagtggag agagatccgt ccatgctgtg agagaccaag actgccagcc atgccggtaa
      481 tactttaccg gggtggttgt gagctcgatc ttgatttcgc tggctttgag aattttaggt
      541 tctttgattt ctaaaagaga gattttcatg tttgagtttc catcaaattt cgcgctgatt
      601 gtaaaggaaa atgcgtaact acgccaattt tgctcccttg acgtctaaaa taccgtaagg
      661 tatattctgc gtgtcacttt cggaaaagaa gcctaaatcc tatgtatgaa ttcgacaaaa
      721 ctgatataaa aatcgtcaat ttgcttctag aagatgggcg tatgtccgct tctgagatgt
      781 cgcgccggat gggggatatt tcggaacggg cggtacgtta ccgtgtggat cgaatgatcg
      841 aagaaggtgt cattcagatc agtgcggtgg tcaacccaga ggcgctcgga ttcaacatca
      901 aagccgatgt gtggctggaa gtagaagccg acgcggtttt ggaagttgcc aagaaaatgg
      961 cttccttcga aaatgtgacg tacgtagcct gcggcattgg gcagaatgac atcagtattc
     1021 agttggttgc gcgtgatact tcggagattt attattttgt caccgaagtg gtgcgcaagg
     1081 ttccaggggt gcgcaagacc acaacttcga tcgtaccgat cattctcaaa gatgtttacc
     1141 agtggagagt gccagagcgc atagccaaag aattgtcaga gaacgaaaca gtaaccgcat
     1201 ccaagtaatt ctgctacgcg gcgattcgcc gcaacccgat aaaaggagca atcatgttcg
     1261 gttcaaagac tcaggctctc caacagcgtg cgcaaaaagt ggcaccgctg ggggtgaact
     1321 ctaattttcg tttttgggga gatggcatta ctccctatgt tgaaaaagcc aagggcggct
     1381 atttatggga tgtagatgga aagaaataca ttgactatcg catggcattt ggtccgatta
     1441 ttttaggtca tgcctatgct gaagtagacc aaaaagttat cgaagaaatt caaaaaggca
     1501 ttctgtttgc catgaccggc gaacttgaaa ttgccgtggc tgagatgatc gttgacatgt
     1561 caccagccat cgacatggtg cgcttggctt gttcagggac tgaggcaacc atgcatgcta
     1621 ttcgtgtggc gcgtgcctac actgggcgcg atatcatcct caagttcgag gggaattatc
     1681 acggcttcca cgaccatacc ttgtggtcaa cctacgcacc ggtagaagcc tacggcaatc
     1741 gtcgtagccc aattcctgtg cccgcgtctt ctggtattcc caagatgatg cgtgaaaata
     1801 tcatcacgct gcctttcaat gattttgaag gcttcgagcg tgtgatgcgt tc
//
LOCUS       DAOREF010000701         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_53259_length_1847_cov_4.986049, whole genome shotgun sequence.
ACCESSION   DAOREF010000701 DAOREF010000000
VERSION     DAOREF010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53259_length_1847_cov_4.986049"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKI33_13215"
     CDS             <1..505
                     /locus_tag="PKI33_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38022.1"
                     /translation="DTALGFFGESVWSSDAVMLAFATNDGNVIHIFQNNKEILTLDFL
                     NDKSLNSNFREGISISELKWSLNDQRLLIGTYDDNILLLDINEQILYKLCFENKEINN
                     YAWDNFFYLKSGQHIVAPVNLRSQDYHYEQFDVLIDTTTMRAYRLPTSDLNGRIGWLA
                     LPESAEK"
     gene            507..788
                     /locus_tag="PKI33_13220"
     CDS             507..788
                     /locus_tag="PKI33_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38023.1"
                     /translation="MNFLKKLFAPREPYKKEYYTFNVKCKRCGETITGRVDLDNDLSI
                     EYEDGGDVFYARKVLMGENKCFQRIEVNLKFNADRQVIEKDIIGGDFIE"
     gene            785..1243
                     /locus_tag="PKI33_13225"
     CDS             785..1243
                     /locus_tag="PKI33_13225"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNM38024.1"
                     /translation="MTDSQIETYWEAFLAAHPEYRGKPYLAEPFGDNPGLADELGNLV
                     LSGLKSATCSAVWNYEAEGQPFPQVGTLWLVLSGRNEPMCVVETVEVTFRKYNEVDAD
                     FARAEGEDDLSLESWRAGHKRFFTRMLATIGKEFVEDMPLVCERFRVIYK"
     gene            1256..>1847
                     /locus_tag="PKI33_13230"
     CDS             1256..>1847
                     /locus_tag="PKI33_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38025.1"
                     /translation="MKHSPNFLLACVLSFSMIACGRGILPQQSSVIAEAPDTPKPIEK
                     TLSSFTPIIGTDYLMAGIVPIEVTRDSTLNPFEWISSSGYSSYTSYATYNYVFFNTQT
                     EEYHRLLPDNNSVILQTAGFPQQVYDPNDPQKPAPVIEFWMFNIIKADTNGDTYFDYR
                     DKLTIGISDVSGSGYTELIQNVDVIQSQYYKDPSNFF"
BASE COUNT          538 a          453 c          377 g          479 t
ORIGIN      
        1 cgatactgca cttggctttt ttggtgagag tgtttggtcg tctgatgctg ttatgctagc
       61 atttgcaact aatgacggaa atgtaattca catatttcaa aataacaaag agatattgac
      121 cctcgacttt ttgaacgaca aatcattaaa ttcaaatttc agagaaggga tatccattag
      181 tgaactaaaa tggtcattaa atgatcagag gctacttata ggcacctatg acgacaatat
      241 ccttttatta gatattaatg aacaaatatt gtataaactt tgttttgaaa acaaggaaat
      301 taataattat gcatgggaca acttctttta tttaaaaagt ggacagcata tcgttgctcc
      361 ggtgaatttg agaagtcaag actatcacta cgagcaattc gatgtgctca tagatacaac
      421 gacaatgcgg gcatacaggt tacctacatc tgacttaaat ggtcgcatcg gctggcttgc
      481 attacccgag tcagcggaga aataaaatga atttcctcaa aaaacttttc gctccccgtg
      541 aaccctataa aaaagaatat tacactttta acgtcaaatg caaacgctgc ggtgagacga
      601 ttactgggcg cgtggacctc gacaacgacc tcagcattga gtacgaagat ggcggcgatg
      661 ttttctatgc tcgcaaagta ttaatgggcg agaacaaatg ctttcagcgt atagaagtaa
      721 acctcaagtt caatgcagat cgccaagtga tcgaaaaaga catcatcggc ggagatttca
      781 tcgagtgacc gactcgcaaa ttgaaaccta ttgggaagcg ttccttgccg cccatcctga
      841 atatcgcggc aagccgtacc ttgccgaacc ctttggtgat aaccccggcc ttgccgatga
      901 actcggcaac cttgtcctca gtggacttaa atccgccacc tgttctgccg tgtggaacta
      961 cgaagccgaa ggtcagccgt ttccacaagt gggcacacta tggctcgtgc tcagcggacg
     1021 caatgaaccg atgtgcgtgg ttgaaactgt cgaagtgacc tttcgcaaat acaatgaagt
     1081 ggatgcagat tttgcccgtg ccgaaggcga agatgatctt tctctggaaa gctggcgtgc
     1141 agggcacaaa cgcttcttca cccgtatgct tgcaacaatc ggaaaagaat ttgtagaaga
     1201 tatgccgctc gtttgtgaac gcttccgcgt catttacaaa taaagtgaga tcctcatgaa
     1261 acactctcca aattttctgc ttgcatgtgt cctatcgttc tccatgatcg cctgtggacg
     1321 cggcatccta ccgcaacaat catctgtcat tgccgaagcg ccagataccc ccaaacccat
     1381 tgaaaagact ctttcctcat tcactcccat tatcggcacc gactatctca tggcaggcat
     1441 tgtgcccatt gaagtaaccc gcgactccac tctcaatccc tttgagtgga tcagcagtag
     1501 cggttactca agttacacat cctacgccac atataactat gttttcttca acacccaaac
     1561 cgaagaatat catcgcctcc tgccagataa taactctgtc atcttgcaaa ctgccgggtt
     1621 ccctcaacaa gtgtatgatc ccaatgaccc gcaaaagccc gcgcccgtga tcgaattttg
     1681 gatgttcaac atcatcaaag ccgataccaa tggcgacacc tactttgact atcgcgacaa
     1741 actcaccatt ggcatttccg atgttagcgg cagcggctat accgaactaa tccaaaatgt
     1801 ggatgtgatc caatcgcaat actacaaaga cccatccaac ttcttca
//
LOCUS       DAOREF010000702         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_53304_length_1846_cov_3.756561, whole genome shotgun sequence.
ACCESSION   DAOREF010000702 DAOREF010000000
VERSION     DAOREF010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53304_length_1846_cov_3.756561"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKI33_13235"
     CDS             complement(<1..353)
                     /locus_tag="PKI33_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38026.1"
                     /translation="MKKYVPILKSIVLYTLLTGATVLMSFPLFWMISSSFKTVEETNS
                     PGVVWIPREPTVEAYTAIFKNPDFLRSYFNSVFYVTLALVGTLLSIAAVAYAFSRVDW
                     PGRNVVFFLMLSTMM"
     gene            complement(363..1517)
                     /locus_tag="PKI33_13240"
     CDS             complement(363..1517)
                     /locus_tag="PKI33_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM38027.1"
                     /translation="MPENPYLNVLLMAALTVVFIIACSYGLGHLAKSIARLRGASPKK
                     QESTFAGYFFAAPWIVGFLIFVIVPLGFSLYWSFTDYRIASGEPTLWVGAKNYQTILF
                     DDESFRSSIINTLYLTVIGLPLQIGVALFLAVLLNQKMRGERIFRMAFYLPVILGFNT
                     AVLLCWRLMLNTGTGIINQIIRGITEAFPPFGYISRGIIYIQEVSAAFFLGLNTGKYN
                     LMMKVIEAGFPAENRVPLWVQSPLWTKMSVVLLMVWGCGAMMLIFLAGLNNIPKELHE
                     AAEVDGASPWQRFWKISFPLLTPYIFYNLVVGMISALQIFEPIFVLYRDNQPVVSSTI
                     SMVYYLWQKSFSHFEIGYGSAISWVILVIIMIVTVIQFRLQDRWVTYDIY"
     gene            complement(1581..>1846)
                     /locus_tag="PKI33_13245"
     CDS             complement(1581..>1846)
                     /locus_tag="PKI33_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38028.1"
                     /translation="GNNKWNETMGLIPARNDSQYGYVTESAQLQREAELASQYGVGFA
                     GIKEAAKLSTIMQDALGKLITEELTVDEVNAKIQEEYTAALGQ"
BASE COUNT          563 a          429 c          439 g          415 t
ORIGIN      
        1 atcatcatgg tggaaagcat tagaaaaaaa actacattac gcccgggcca gtccacgcgg
       61 ctgaaggcat aggcaaccgc cgcaatggaa agaagtgtac ccaccaaagc aagggtaacg
      121 taaaagaccg agttgaaata ggatcgcaga aaatccggat ttttgaagat cgcggtgtaa
      181 gcttccactg tcggctcgcg cggaatccag accacgccgg gcgagttggt ttcctctact
      241 gttttgaatg aggaggagat catccaaaac agtgggaaac tcatcaagac cgtcgccccg
      301 gtcaacaagg tgtaaagcac aatgcttttg agaatgggaa catatttttt cattgtgctc
      361 tcctaataaa tatcgtaggt tacccagcgg tcttgcaagc ggaactggat aactgtaacg
      421 atcataataa tgacaagaat gacccatgaa atagcagagc catacccgat ttcgaaatga
      481 ctaaagctct tttgccaaag gtaataaacc atggaaatgg ttgaagaaac caccggttgg
      541 ttatcacgat agaggacgaa gatcggttca aagatctgta aggcactgat catgcctaca
      601 acaaggttat aaaaaatata cggggtgaga agtgggaagg aaatcttcca gaaacgctgc
      661 caagggctag ctccgtctac ttcggcggct tcatgcaatt ccttgggaat attgttcaat
      721 cccgcgagaa agatcaacat catggcgccg cagccccaga ccatgagcaa gacaaccgac
      781 atttttgtcc acaacgggct ttgcacccaa agtggaacgc ggttctcagc cggaaagcct
      841 gcttcgataa ccttcatcat caggttgtat ttgccagtat tcaagccgag gaagaatgcg
      901 gcagaaactt cttgtatata gataatgcca cggctgatat agccaaatgg cgggaaagct
      961 tcggtaattc cgcgaataat ttgattaata atgcccgtgc ctgtgttcaa catcaagcgc
     1021 caacacaaaa ggacagccgt gttgaagccc aaaataacag ggagataaaa cgccatacgg
     1081 aagatacgct caccacgcat tttttggttg agcaacactg ccaaaaacaa tgctacgccg
     1141 atctgcaaag gcaaaccaat cacagtgaga tataaggtgt tgataatgga agagcgaaaa
     1201 ctttcgtcgt caaaaagaat agtttgataa ttttttgccc ccacccacaa ggtgggctcg
     1261 cctgaggcga tacggtaatc ggtaaaactc caatagagag aaaacccgag tggaacaatc
     1321 acaaaaataa ggaagccaac aatccatggt gcggcaaaaa aatagcccgc aaaggtactt
     1381 tcttgttttt tcggagatgc gccgcgtagc ctggcaatag acttggctag atgccccaac
     1441 ccataactac atgcaatgat gaacacgact gtcaatgctg ccataagcag cacattcaga
     1501 tatggatttt caggcatggc gagtctcctc taaaaatatt gctcgcatgg ggcaagcccc
     1561 atgcgagcaa taaagtttct ttactgaccg agagcggcag tatattcttc ctggatcttt
     1621 gcgttcactt cgtcaacagt aagttcttcg gtaatcaact tgccgagagc atcctgcatg
     1681 atggtggaga gcttggcagc ttccttgatg ccagcgaagc cgacaccgta ctgggaagcc
     1741 aattccgctt cacgctggag ctgggcagat tcggtcacat aaccatactg tgagtcgtta
     1801 cgggcaggga tcaagcccat ggtctcattc cacttgttgt tgcctt
//
LOCUS       DAOREF010000703         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_53417_length_1844_cov_2.846842, whole genome shotgun sequence.
ACCESSION   DAOREF010000703 DAOREF010000000
VERSION     DAOREF010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53417_length_1844_cov_2.846842"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PKI33_13250"
     CDS             <1..1005
                     /locus_tag="PKI33_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNM38029.1"
                     /translation="DILGWVHSEGAKTSFQFKHGGDAIRAVKVGDGVHAQPQITTDGV
                     VILFEDVNHPTDLWKIDLESGSTSQLTKSLDVDLNFQQPEEIWYTSKDGVQVPALLYR
                     GSGENAVLDIHGGPNWHVQFSWNPVLSLMVQHGWTVLVPNYRGSTGYGKKWQNASRYD
                     MGGVDNDDCAAGANYLLKNNLAKKIAVTGRSHGGYLTMTCLTGYPELFVGGSAVVPFL
                     NWIKSHHESREDLKHWNIENMGDPDDNRELWIARSPYFFLDKVTSPVQMICGENDPRC
                     PASDSMDARDKLIELGKEVEFLLYKGEGHSFYKIENIIDAEMKRMGFLERVLTTDSKQ
                     "
     gene            1037..>1844
                     /locus_tag="PKI33_13255"
     CDS             1037..>1844
                     /locus_tag="PKI33_13255"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNM38030.1"
                     /translation="MEPLKFLSDADVQAMHEATLQIMSEVGIIWTHKPSLDVLLQAGC
                     KMKDNRVLFPPDLVMDSVAKANKRPKIRGRNGTVNELGGGNLYFHNLGGARDVFDART
                     GTHHTATAEDCVNAVRLLDALPNCNNVTPFFTPPDVENEMMALHMFRHALSNTTKPVQ
                     GPGIQFGHEAKYAVEMAKVIGIEPHEITLSLSPVSPLTMHDIAAQAIMDMAQAGVIHA
                     NLPAPTGGATSPMTITGSLVQQNAETLAPLVLAQILNPGCGVVYCGRLRLL"
BASE COUNT          497 a          417 c          478 g          452 t
ORIGIN      
        1 gacattctcg gctgggtaca cagtgaaggt gcaaagacca gtttccagtt taagcacgga
       61 ggcgatgcca ttcgtgcagt gaaggtcggc gatggagtcc atgctcaacc gcagattaca
      121 accgatggtg tcgtcatcct atttgaagat gtaaatcacc ctaccgattt atggaaaatc
      181 gatcttgaaa gcggaagtac ctcacagttg accaaatcac tagatgtgga cttgaacttt
      241 caacagcccg aagagatttg gtacaccagt aaggatggcg tgcaagtgcc tgctttgttg
      301 taccgcgggt ctggtgaaaa tgctgtgctt gatattcacg gcggaccaaa ctggcatgtg
      361 cagttttcat ggaatcctgt gttgagtttg atggtgcaac atggttggac tgtgcttgtg
      421 ccaaattatc gtggctcgac agggtatggc aaaaaatggc agaacgccag ccgttacgat
      481 atgggcggcg tggataatga cgactgcgca gcgggggcaa actatttatt gaaaaataat
      541 ttagcaaaga agattgctgt aactggtcga agccatggtg ggtatctcac tatgacttgt
      601 ttgacgggat accccgaatt gtttgtgggc ggctcagcgg ttgtgccgtt cttaaattgg
      661 ataaaatcac atcacgagtc acgtgaagac cttaaacact ggaatatcga aaacatgggc
      721 gaccccgacg ataaccgtga gttgtggatt gcccgctctc catatttctt tttagacaaa
      781 gtcacatcgc ctgtgcaaat gatctgtggc gaaaacgacc cgcgctgtcc ggcatctgac
      841 tctatggatg ccagagacaa attgatagaa ctggggaaag aagtagaatt tctgttatac
      901 aaaggggaag gacattcgtt ttataagatc gagaatatta tcgatgcaga aatgaaacgt
      961 atgggatttt tagaaagagt attaacaaca gatagcaagc aataagtatg cctacatttt
     1021 taccgaggag ataaccatgg agcctttaaa gtttttgtct gacgcggatg tacaagccat
     1081 gcatgaagcc actttgcaga ttatgagcga agtaggcatt atttggactc acaaacccag
     1141 cctcgatgtt ttgttacaag caggctgcaa aatgaaagac aaccgcgttt tgtttcctcc
     1201 cgacctcgtg atggattcgg ttgcaaaagc gaataaacgc ccaaagatcc gtgggcgaaa
     1261 tggcacggtc aacgaattgg gcggcgggaa tttatatttc cataacctcg gcggcgcacg
     1321 tgatgtgttt gatgcacgca caggcacaca tcacaccgca accgcagaag attgcgtgaa
     1381 tgcggttcgt ttgttagacg cactcccaaa ctgcaataac gttacaccat tttttactcc
     1441 gccagatgtg gaaaatgaaa tgatggcatt gcatatgttc cgccatgcgc tctcgaacac
     1501 caccaaacca gtgcagggtc ctggcattca gtttgggcat gaagcaaaat acgccgtgga
     1561 aatggcaaag gtcatcggta ttgagccgca tgagatcacg ctttcgcttt cgccggtcag
     1621 tccgctgacc atgcacgata ttgccgccca agccatcatg gatatggcac aagcaggcgt
     1681 tatccatgcc aacctgcctg cccccactgg cggggcgacg tcgcctatga ccattacggg
     1741 cagtctcgtc caacaaaatg cagaaacgct ggctccgctc gtcctcgcac agattttgaa
     1801 cccaggctgt ggtgtggtct attgcgggcg tcttaggctg ctgg
//
LOCUS       DAOREF010000704         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_53682_length_1839_cov_3.945628, whole genome shotgun sequence.
ACCESSION   DAOREF010000704 DAOREF010000000
VERSION     DAOREF010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53682_length_1839_cov_3.945628"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKI33_13260"
     CDS             <1..869
                     /locus_tag="PKI33_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNM38031.1"
                     /translation="KFICAVGSGPNNIRAEARFETTTPQETMGHVIEFFKQQEENLGG
                     LSAIGFASFGPLDPNPASVTYGHILPTPKPGWTNANVVGMLRNEFDVPIAFDTDVNGA
                     ALGEWRWGKAQGLQTFIYLTIGTGIGGGAYVEGNLLHGLVHPEMGHILVKHDLEKDPF
                     EGVCPFHGDCFEGLASGVAIEKRWGMRGALLTPDHIAWDLEAEYIAQALANYVFTLSP
                     QRIIIGGGVGNLAHLLPKVQRRTRELINGYVQSPAILENIESYIVNPGLGNRSGMLGA
                     IALAEQSLLRAS"
     gene            866..>1839
                     /locus_tag="PKI33_13265"
     CDS             866..>1839
                     /locus_tag="PKI33_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNM38032.1"
                     /translation="MIELNQLELEASKSLVRILPRVEQNLKRFITKDPQAWVQFTTRL
                     QKNFPALLGFYADVYSYRYDFFYHLEDLVMSMARSWFERPEDLRELDDAREQNPHWFQ
                     SNQMLGGVCYVDLFANNLDGIRKKIPYFKELGLTYIHLMPLFKAPEGENDGGYAVSSY
                     REVHPPLGTMKQLSSLARDFREAGISLVVDLVFNHTSDEHIWAERAKQGDEEYMDFYR
                     IFPSRELPSLYEQNLREIFPDEHPGAFSPITINGKEHWVWTTFHYYQWDLNYSNPAVF
                     NRMAEEMLFLANQGVEVLRLDAVAFIWKQLGTNCENLPQAHTLIRAFNA"
BASE COUNT          438 a          484 c          464 g          453 t
ORIGIN      
        1 caaaatttat ttgcgcagtg ggcagcggac ccaacaacat ccgtgctgag gcgcggtttg
       61 aaacgaccac tccccaagaa accatggggc atgtgatcga gtttttcaaa cagcaggaag
      121 agaatctggg agggctttct gctattgggt ttgccagttt cggtccattg gacccgaacc
      181 cggcatctgt tacatacggg cacattctgc cgaccccaaa acctggttgg acgaatgcca
      241 atgtggtggg catgttacgc aatgagttcg atgtaccgat tgcatttgac acggatgtga
      301 acggtgccgc attgggtgaa tggcgctggg gcaaagcaca agggcttcag accttcatct
      361 atctcaccat tggcacgggc attggtggcg gcgcttatgt agaaggcaac ttgctgcatg
      421 gattggtaca ccctgaaatg ggtcatatct tggtgaagca tgatctggag aaagacccct
      481 tcgaaggcgt ttgtcccttc catggtgatt gtttcgaagg gttggcttcg ggcgttgcca
      541 ttgaaaagcg ttggggcatg cgcggcgcct tgctcacacc cgatcatatc gcctgggact
      601 tggaagccga atatattgca caagctctgg caaattatgt atttacactt tcgccgcagc
      661 gcatcattat cggcggtggc gtaggcaact tggcacacct cctgcccaag gtccaaaggc
      721 ggacgcgtga actgatcaat ggctatgttc aatcacctgc catccttgaa aacatcgaat
      781 cctatatcgt taatcccggt ttgggcaatc gctctggcat gttgggcgcc attgcacttg
      841 ctgaacaatc cttattaaga gcatcatgat cgaacttaat caactcgaac ttgaagccag
      901 taaatcgctc gtgcgcattt tgccgcgtgt agagcaaaac ctgaaacggt tcatcaccaa
      961 agacccgcag gcgtgggtgc aattcacaac ccgcttgcaa aagaatttcc cggcattgtt
     1021 gggtttttat gccgatgtct atagctaccg ttatgatttc ttttatcacc tcgaagacct
     1081 tgtgatgagc atggcgcgtt catggttcga acgtccggaa gatttgcgcg agttggatga
     1141 tgcccgtgag caaaacccgc attggttcca atcgaaccaa atgttgggcg gcgtttgcta
     1201 cgtggacctc tttgccaaca acctcgatgg cattcgcaaa aagattcctt acttcaaaga
     1261 acttggtctt acctacattc atctcatgcc gctcttcaaa gcgcccgaag gcgagaacga
     1321 tggcggctat gccgtcagca gttatcgcga ggtgcatccg ccgcttggta ccatgaagca
     1381 gctttcatcc cttgcgcgtg attttcgtga agccggcatc agcctcgtcg ttgacctggt
     1441 cttcaatcac acctccgatg aacacatctg ggcggaacgt gccaagcagg gcgacgaaga
     1501 atacatggac ttctaccgca tcttccctag ccgtgagctg cccagcctct acgagcagaa
     1561 tctcagggaa atctttcccg acgaacaccc cggcgctttt tctccgatca ccatcaatgg
     1621 caaagaacat tgggtgtgga caaccttcca ctactatcaa tgggatctga attattccaa
     1681 cccggcagtc ttcaaccgca tggcagaaga aatgctcttc ctcgccaacc agggtgtgga
     1741 agtattgcgc ctggatgccg ttgctttcat ttggaaacaa ctcggcacca attgcgaaaa
     1801 cctgccgcaa gcgcataccc tgattagagc tttcaatgc
//
LOCUS       DAOREF010000705         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_54060_length_1832_cov_4.746764, whole genome shotgun sequence.
ACCESSION   DAOREF010000705 DAOREF010000000
VERSION     DAOREF010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54060_length_1832_cov_4.746764"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PKI33_13270"
     CDS             <1..1554
                     /locus_tag="PKI33_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNM38033.1"
                     /translation="QPKARNFRPELALGTQTPQGNSIVFQVYVPLEAKKFPRLYWRGQ
                     VYDRYENGRWLTTSDAEVRQNPATGDITIPDSTDRQRLSFTFDVMTSGQTTVYLPPQP
                     IWINHSTILLSSDISNKTGEDAILDIMAVRASPPFQDGDLYRVSSLLADPTAEELRLS
                     GDAYPDWVKEKYLQLPENFSPRIQALAQQITAPYNTPYDKTVAVTNYLRREIEYTGVI
                     YLPNEDVDPLEYVLFDAKRAFCNYYATAQVLMLRSVGIPARLAVGYAQGQPNIQKSLY
                     AVREKDLHAWPEVYFPEYGWIEFEPTTNQQPIERPEAREDLAQIAPFANNPNQPLFKP
                     DVEFTPDQEPNVPDGGSASARSWLDWLASLLPWLGGVFFILLGIVLKRRFAPQVTVAS
                     VMKRAIDRSGWNAPRWLRRWLAFATQPAIQRHFHNINISLRLLKRPQPVHVTAAERAQ
                     TLKGLLPSASASIDSLLNELQAELFTPNGGNEAIARRAAWDILSKTVQSKLKIIILGY
                     NYEGIKETS"
     gene            1561..>1832
                     /locus_tag="PKI33_13275"
     CDS             1561..>1832
                     /locus_tag="PKI33_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38034.1"
                     /translation="MIENSTRPRTVHDQLSALAVLAGKIRAELGNSKLKVPKESLEQL
                     HYLQSTLETVGNKMEVFQREHGNMLALVETGQVINSSLELDEVLRIV"
BASE COUNT          484 a          474 c          421 g          453 t
ORIGIN      
        1 cagcctaaag cacggaactt ccgcccagag ttagcccttg gcacacaaac cccacaaggc
       61 aattcgattg tctttcaggt gtatgttccc ctcgaagcaa agaaatttcc gcgcctctac
      121 tggcgcggac aagtgtatga ccgttatgaa aacggacgtt ggctaacaac cagtgatgca
      181 gaagttcgtc agaaccctgc cactggtgat attactatcc ctgatagcac agaccgtcag
      241 cggctttctt tcacctttga cgtgatgacc tctgggcaga cgactgttta tcttccaccg
      301 cagcctatat ggattaatca cagcacaatt ctgctttctt cggatatttc gaataaaact
      361 ggagaagatg ccattcttga tattatggca gttcgcgcct ctcccccatt tcaagatggg
      421 gacttgtacc gcgtcagttc cttgcttgca gacccaacag cagaagaact gcgtttaagt
      481 ggagatgcat atccggattg ggtcaaagag aaatatcttc aactgcctga aaacttctcg
      541 ccacgcattc aggcactcgc tcagcaaatc acagctccct ataacacccc ctacgacaaa
      601 acagtcgctg ttacaaacta tctgcgcagg gaaattgaat acactggtgt gatttacctt
      661 cccaatgaag atgttgaccc gcttgagtat gtattgtttg atgctaagag agctttctgc
      721 aattactatg ccacggctca ggttctcatg ctacgcagtg ttgggatccc tgcccgtttg
      781 gcggtgggat atgcccaagg gcagccaaat attcaaaaaa gtttgtatgc ggttcgtgag
      841 aaagacctgc atgcctggcc cgaagtctat ttccctgagt atggttggat tgaatttgaa
      901 cccaccacaa atcaacaacc gatcgaaaga ccagaagctc gcgaagattt agctcaaatt
      961 gctcccttcg caaacaaccc aaaccagcca ctatttaaac cagatgtgga attcacgcca
     1021 gaccaagaac caaatgtgcc agacggaggg tcagcttcag cgaggtcttg gctagattgg
     1081 ttagcatcgc tcttgccgtg gttgggaggc gtgttcttta tcctccttgg aattgttctc
     1141 aaacgacgct ttgctccaca ggtgaccgtt gcttctgtga tgaaacgagc aatagaccgt
     1201 agcggctgga acgcgccacg ttggctgcgt cgttggctag cgttcgctac ccagcctgcg
     1261 atccaaaggc attttcataa tatcaatatc agccttcgat tattgaaacg cccacagccc
     1321 gtgcatgtga ctgcggcaga acgggcacag accctaaagg ggttgctgcc cagtgcgtct
     1381 gcatcaatcg acagcctttt gaacgagctt caagctgagt tattcacccc taacggtggg
     1441 aatgaggcaa ttgcccgccg tgcagcatgg gatatccttt ctaaaaccgt acaaagtaaa
     1501 ctaaaaatta tcatcttggg atacaattac gagggtatca aggaaacctc gtaacacccc
     1561 atgattgaga actccactcg tccccgcacc gttcacgatc aactatctgc ccttgccgtg
     1621 cttgccggta agattcgcgc ggaacttggc aactcaaaat taaaggtgcc gaaggaatcg
     1681 ttagaacaac tacattactt gcagtctacc ctggaaacag ttggcaacaa aatggaagtg
     1741 ttccaacgtg agcatggcaa tatgcttgcg ctcgttgaaa cagggcaggt cattaattca
     1801 tcccttgagt tggatgaagt gctgcgcatc gt
//
LOCUS       DAOREF010000706         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_54114_length_1831_cov_3.403716, whole genome shotgun sequence.
ACCESSION   DAOREF010000706 DAOREF010000000
VERSION     DAOREF010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54114_length_1831_cov_3.403716"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKI33_13280"
     CDS             complement(<1..915)
                     /locus_tag="PKI33_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNM38035.1"
                     /translation="MRLSTKLILSFMLISIFSTGIIVVSSRILASREFERFVGDRYTA
                     DLADDLARYYSRNQTWDGVEQEFQHGDHDHHSLYFSIADPQGNIVVAGNEYNVGDPCS
                     QEEFEEGYPIQSNNKTVGVLLLPSAPEQNPLDYEFLRRLNGSIFLSAIATLVFALLIG
                     ILLSRSILRPVRELTKATHHMADGNLGGQVPVRSRDEIGELAQSFNKMSADLARSFNL
                     RKQMTADIAHELRTPISLILGHAEGVKDGVLKPNAENFEIIREEAERLEHLVSDLRTL
                     SLADAGELSVDFQPVDINNFMSDIHAHYL"
     gene            complement(926..1609)
                     /locus_tag="PKI33_13285"
     CDS             complement(926..1609)
                     /locus_tag="PKI33_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM38036.1"
                     /translation="MPQKILIVDDERRMVSLLQSYLEQEGYHVLTAYNGREALEVARR
                     ENPDLVVLDIMMPEMNGYEFMNARRAESDTPIIMLTAKVEDEDKIIGLELGADDYVTK
                     PFKPRELMARVRNVLRRAGKNEAKGQTLKVLDITLNRETREVSIGDRHADLTPSEFDL
                     LAALMTAPGKVFSRLDLLDVIQGVRYEGYERTIDTHVKNLRVKVEDDPRKPSHIETVY
                     GVGYRLRKQ"
BASE COUNT          466 a          439 c          440 g          486 t
ORIGIN      
        1 caagtaatga gcgtggatgt cgctcataaa gttattgatg tctacaggtt gaaaatccac
       61 agaaagttca cccgcatcgg cgagcgacaa agtccgcaga tcgctgacaa gatgttcgag
      121 gcgttcggct tcttcgcgga tgatctcaaa attttcggca ttcggtttta gaacaccgtc
      181 ttttacacct tcagcgtgac caagaataag gctgatgggc gtgcgaagtt catgcgcaat
      241 atcggcggtc atttgtttac ggaggttgaa ggagcgtgcc aagtctgcac tcatcttatt
      301 gaatgattgc gccaattcgc caatctcatc tcgcgagcgg acaggaactt gtccgcctag
      361 atttccatct gccatgtggt gagtggcttt ggtcagctcg cgaactggtc gcaaaatact
      421 gcgcgacagc agaataccaa tcaataatgc aaagaccaaa gtagcaatgg cgcttaagaa
      481 aatagagccg tttaaccggc gcagaaattc ataatcaagc ggattctgtt ctggcgcgct
      541 aggcaacaag agcacaccga ctgttttgtt gttcgattga atggggtagc cctcttcaaa
      601 ctcttcctga ctgcacggat caccaacatt gtactcattg cctgcaacga ctatattgcc
      661 ttgcgggtct gcaattgaaa aataaagcga atgatgatcg tggtcaccat gttgaaattc
      721 ttgttccaca ccatcccagg tttgattacg gctgtaatac cgagccaggt cgtctgccag
      781 gtcagcggtg tagcgatcgc caacaaagcg ttcgaattcg cggctggcaa gaatgcgcga
      841 agaaacgaca atgatgccgg tactaaatat gctgataagc atgaacgaaa ggatgagttt
      901 ggttgaaaga cgcatgattt tgaaattact gttttctaag gcgataccct acaccataca
      961 cagtttctat atgagaaggt tttcgtgggt catcttctac tttaactctt aagttcttga
     1021 cgtgagtatc aatcgtgcgc tcatatcctt cgtagcgcac accttgaatt acatcgagca
     1081 ggtcaaggcg cgaaaaaact tttccggggg cggtcatgag cgctgcaagc aggtcaaatt
     1141 cagaaggggt taagtctgcg tggcggtcac caatgctgac ttcgcgtgtc tcacggttga
     1201 gtgtgatatc gaggactttc aacgtttgcc ctttggcttc gttcttgccg gcacggcgca
     1261 atacattgcg aacacgagcc atcaactcac gaggcttgaa aggtttggtc acataatcat
     1321 cggcaccaag ttcgagtcca atgattttat cttcatcttc cactttcgcc gtcagcataa
     1381 taataggcgt gtcgctttcg gcgcggcgcg cattcataaa ctcgtaaccg ttcatttccg
     1441 gcatcataat atcaagcaca acgaggtctg ggttttcacg gcgggctact tccaaggctt
     1501 cacgcccgtt atacgccgtt aatacgtgat acccttcttg ctcaagataa ctttgcaaga
     1561 gagaaaccat tcgtcgttca tcgtctacga taagaatttt ctgaggcatg aatcgattat
     1621 cccataaacc ctgtgaagaa attgtgtgaa atttaataga ataagaacac ccgcctcatt
     1681 ccaaggcggg tgctgctgcg gcgttagaac agtgggttac gcctcatcct ttttctcttc
     1741 gctttcaacc tgcacttcag cagcttccgg cgctacgtct ggtgctgggg cagcagcttg
     1801 tgagttgttg acattcacaa gtcgccatcc g
//
LOCUS       DAOREF010000707         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_54130_length_1831_cov_2.672297, whole genome shotgun sequence.
ACCESSION   DAOREF010000707 DAOREF010000000
VERSION     DAOREF010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54130_length_1831_cov_2.672297"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="selA"
                     /locus_tag="PKI33_13290"
     CDS             <1..849
                     /gene="selA"
                     /locus_tag="PKI33_13290"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNM38037.1"
                     /translation="VEIGGGFRVPDVMKQSGAKLVEIGTTNKVRVADYSIALEEPTAL
                     VMRAHRSNFKIVGFTEEPEFKDMVDAAHKVGVPVVDDLGSGALYDTAKYGFSHEPTVQ
                     ESMQAGADVILFSGDKLLGGPQAGIIIGKKDLLDKIKKHPLARAVRADKSCLAGISAT
                     LTHYLKDEAEREIPIVRMMSLTLKQVKGRAEAWRDELRQGNVVVGQSTVGGGSLPDES
                     IPTFLLELQVKSAEKFLRALRKNNPPIIARTENDKVLLDPRTVFPEQEGALLVGLKNL
                     LKKENK"
     gene            846..>1831
                     /locus_tag="PKI33_13295"
     CDS             846..>1831
                     /locus_tag="PKI33_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNM38038.1"
                     /translation="MKSDLDAIMQSRNLDAFVVFGNAENNPPMYYLTGGGHVSHATVI
                     KKRGEDAVYFYNDMERDEAAKSGLKLTPYSKYDYNELLQKANGDLLLTSALRYQQMFE
                     DVGVTKGRVGVYGHYDVGAVFGWLNHLQKLMPAIEFVGEARDDSIFLRAMETKDENEV
                     ARIRKMGKVTTAVVEKVKEYLTSCDVREDEVLLKEDGTPLSVGDVHSKIRLWVSEHGA
                     ELPSGFIFAIGRDAGVPHSAGNPADLMRLGQTIVFDIYPAEAGGGYFYDFTRTWSLGY
                     TTPEAQSLFDQVKEIYDTLNDNFDLNVAFKDYQRMTCEFFEGKGHQTPLNTKA"
BASE COUNT          475 a          379 c          502 g          475 t
ORIGIN      
        1 gtggaaatcg gcggcggctt tcgcgtgcca gatgtaatga agcaatcggg cgcaaagtta
       61 gtagagatcg gtaccaccaa caaagtccgc gtggcagatt actccatcgc actggaagaa
      121 ccaaccgctt tggtaatgcg tgcgcatcgc tcgaatttca agatcgtggg ctttactgaa
      181 gagcctgaat tcaaagatat ggttgatgct gcacataaag ttggtgttcc tgtcgtggat
      241 gatcttggct cgggtgcttt gtatgacacc gctaaatacg gtttctctca cgaaccgacg
      301 gtacaagagt ccatgcaagc cggcgcggac gtgattcttt tctccggcga taaactgctt
      361 ggtggtccgc aggcgggcat tatcatcggt aaaaaggact tactcgataa gattaaaaaa
      421 catccgctgg cgagagctgt ccgtgcggat aaatcttgtt tggcgggcat ctctgctacg
      481 ctcacacatt acctcaaaga cgaggcggag cgagaaattc ccattgtgag gatgatgtct
      541 ctaacgctga agcaggtcaa aggtcgggcg gaagcgtggc gggatgaatt gaggcaaggc
      601 aatgtggtag tgggtcaatc tacagtgggg ggcgggtcgc ttccggatga atccatcccg
      661 acctttttgc ttgaattgca agtgaaatct gccgaaaaat ttttacgcgc cttacgaaag
      721 aataatccgc ctatcattgc tcgaacagag aatgataaag tgctgctcga ccctcggacg
      781 gtttttcctg aacaagaagg tgcgttatta gttgggttga aaaatttgtt gaaaaaggaa
      841 aataaatgaa atcagacctc gatgccatta tgcaatctcg caacttggat gcattcgttg
      901 tatttggtaa tgctgagaat aacccgccca tgtattatct gaccggtggt gggcatgtta
      961 gccatgctac ggtgatcaag aaacgcggcg aagatgcggt ttatttttac aatgatatgg
     1021 agcgcgatga agccgccaag agcggattga agttaactcc ctatagcaaa tatgactata
     1081 acgaattgtt acagaaagcc aatggcgatc ttttgttgac aagtgctttg cggtatcaac
     1141 agatgtttga agatgttggg gtgacaaagg gacgtgttgg tgtatatggt cattatgatg
     1201 ttggtgcggt gtttgggtgg ctgaaccatc ttcagaagtt gatgcccgcg attgagtttg
     1261 tcggtgaagc acgtgatgac tctatctttt tgcgcgccat ggaaaccaag gatgagaatg
     1321 aagtcgcgcg tatccgcaaa atgggtaagg taacgactgc agtcgtagag aaggtaaagg
     1381 aataccttac ctcttgcgat gtacgcgagg atgaggtgtt actcaaggaa gatggcactc
     1441 cgcttagcgt aggagacgtc cactcgaaga tacggctttg ggtttctgaa catggcgcag
     1501 agttgccttc gggttttatc tttgccattg gtcgcgacgc cggtgtgccg cactctgcgg
     1561 gcaaccccgc tgacctgatg cgtttgggtc agacgattgt ttttgatatt tatccggcag
     1621 aagcaggtgg tggctatttc tatgatttca cccgtacgtg gagccttggc tatactacgc
     1681 ctgaagcgca atcattgttt gaccaagtca aagaaatcta cgatacgctc aacgataatt
     1741 ttgacttaaa tgtggcattc aaggattatc aacgcatgac ctgcgaattc tttgaaggca
     1801 aagggcatca aactccgctc aataccaaag c
//
LOCUS       DAOREF010000708         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_54389_length_1826_cov_3.613778, whole genome shotgun sequence.
ACCESSION   DAOREF010000708 DAOREF010000000
VERSION     DAOREF010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54389_length_1826_cov_3.613778"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKI33_13300"
     CDS             <1..354
                     /locus_tag="PKI33_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38039.1"
                     /translation="SSLQVDMVNNGMLSYVDDMEKASLALRTFIDKISTAARGYSGMF
                     DAVKINEKELEAIYQFDAAFFDLADQVKSGLDNVEASMGDEAALPAAIRNITSLSRLA
                     VETFERRSEIVTGSR"
     gene            397..1806
                     /locus_tag="PKI33_13305"
     CDS             397..1806
                     /locus_tag="PKI33_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNM38040.1"
                     /translation="MWHNYINATSIDEVVRILAEKRESARVVAGGTDLVLELERGVRK
                     GVDTLIDVTRIPQLDQITIDEDDVIHLGALVTHNDCAASKLIRTRACPLARASWEVGA
                     PQIRNRGTVAGNLITGSPANDTITPLMALGAKVTLVSDRKTRVVPLKKFYLGVRKTVM
                     EPDEMLVDISFPALTKTQRGTFIKLALRRAQAISLVNAAIILDLKADTVKSASITLGA
                     VTPIITHAPKAEKFLIGKKLNDKNIAAAAELAMKSAKPIDDVRGSANYRREMARVITQ
                     RGLTSIRDGKEQTGMPKKPILLVSEKTTQHGSNIAGSFPENKIETIINGKKYTFTSGH
                     DKTLLRLLREEGMLIGTKEGCAEGECGACTIYLDGQAVMSCLVPAPRAHGAEITTIEG
                     LANGEKLNPVQEKFIEHNAVQCGYCIPGFIMSGAKLLEEKPNPTRNEIEQAITGNLCR
                     CTGYYKIVKAIEEASNSSS"
BASE COUNT          509 a          475 c          427 g          415 t
ORIGIN      
        1 tcaagcctgc aagtggacat ggtcaacaat gggatgctct cttacgttga cgatatggaa
       61 aaagcatcac tggcgttgcg tacttttatc gataagatca gcaccgctgc gcgcggttac
      121 tctggcatgt tcgatgctgt gaagatcaac gaaaaagaac tcgaagctat ttatcaattt
      181 gatgctgcct tctttgatct ggcagaccag gttaagagcg ggcttgataa tgtagaagcc
      241 agcatgggcg atgaagccgc cctgcccgcc gccattcgta acatcaccag tctctcacgc
      301 ctcgcagtgg aaacctttga acgccgctct gaaatcgtga ctggcagtcg ttaagtttat
      361 gtaggggcgg gtcgccccgc ccctacggat attcatatgt ggcacaacta tataaacgca
      421 acatccattg acgaagtcgt tcgtatcctt gctgagaagc gtgaaagtgc ccgcgtcgtc
      481 gcaggtggaa cagaccttgt tcttgaactt gaacgtggcg ttcgcaaagg cgttgataca
      541 ctcatcgacg ttactcgcat tccccaacta gatcaaatca caattgatga agatgatgtt
      601 attcatcttg gtgcactggt cacgcataat gactgtgcgg catccaaact catccgcacc
      661 agagcctgtc cgctggcacg tgcgtcttgg gaggtcggcg ctccgcaaat tcgtaatcgc
      721 ggaaccgtag caggcaacct catcaccggc tctcccgcaa acgataccat tactcctctc
      781 atggcgcttg gtgccaaagt tacattggtc tcagatcgaa aaacccgagt tgttccactc
      841 aagaagtttt atctcggtgt acgcaaaacc gttatggaac ctgatgaaat gctggtggat
      901 atttccttcc cggcattgac caaaactcag cgtgggacgt tcatcaaatt ggcactgcgc
      961 cgcgctcaag ccatctccct cgtcaacgcc gcgatcattc tggacctcaa agcggatacc
     1021 gttaaatctg cctcgatcac actgggagcc gttacaccca tcattaccca tgcaccaaaa
     1081 gctgaaaaat tcctcattgg gaaaaaactc aacgacaaaa atatagctgc cgctgcagaa
     1141 ctagcgatga agtctgccaa gccgattgat gacgtgcgcg gctctgctaa ctatcgccgc
     1201 gaaatggcac gtgtcatcac ccaacgcggg ctgacatcca ttcgcgacgg caaagaacaa
     1261 acaggtatgc ccaaaaagcc aatcttactg gtgagcgaaa aaacaactca acatggctct
     1321 aacattgcag gttctttccc tgaaaataag atcgaaacga ttatcaatgg caagaaatac
     1381 acgttcacaa gcgggcacga caagaccctg ttacgtttgc tacgcgaaga aggaatgttg
     1441 atcggcacca aagaaggctg cgccgagggc gaatgcgggg catgtactat ctatttagac
     1501 gggcaagccg tcatgtcttg ccttgtccct gccccacggg cacatggcgc agaaattacc
     1561 accatcgaag gtttggcaaa tggagagaaa ctaaatccgg ttcaagaaaa gtttattgag
     1621 cacaatgccg ttcaatgcgg gtactgcatc cccggcttta tcatgtctgg tgcgaaactt
     1681 cttgaagaaa aaccaaaccc aactcgcaac gagatcgaac aagccattac cggcaatctc
     1741 tgccgttgta ccggatatta caaaatagta aaagccattg aagaagcaag caattcatcg
     1801 tcttaagagg tatgaatgag taaata
//
LOCUS       DAOREF010000709         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_54701_length_1821_cov_2.967157, whole genome shotgun sequence.
ACCESSION   DAOREF010000709 DAOREF010000000
VERSION     DAOREF010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54701_length_1821_cov_2.967157"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKI33_13310"
     CDS             complement(<1..549)
                     /locus_tag="PKI33_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38041.1"
                     /translation="MDAIQKTLENDKELKTAQTRLEKNSAENQQALKTLKNIELEAET
                     LRIKIEQSNSSLYGGTVKNPKELQDLQKEIESLKKHQETLEERQLEAMSQSENTEAEV
                     EKAKAELEVIRSRLGNEHKKLLDEQAELSKQMASFGQEREAALSPIDVSLLTSYEDLR
                     KQKRGVAVTEVEDGACASCGTTI"
     gene            complement(714..>1821)
                     /locus_tag="PKI33_13315"
     CDS             complement(714..>1821)
                     /locus_tag="PKI33_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNM38042.1"
                     /translation="SASISEDRLREFLQNEIASRYDIPPAPAQPVPGGTTFLPGAPGQ
                     TLDVNRAVVIITNALRSPADRSVVLTFNQTTASRPTIENLEILLKQIITTSDFDGLIG
                     YYMMDLQTGQEIHFLINNKQEITVEPDVAFTASSTIKLPILTSYLINHGTNLDTATTE
                     AITRVFRISDNSATDSILERIDPNNGPIIVSQNMEQLGLTSTYISGMFFLGAPNLIPG
                     KVTPGNSRADIFTDPDPYSQTTPSEMGTLLADIYQCVQNGGGALIAAFPDKVTPDTCK
                     LIIDFLALDKFGALIQGGVPDGTLVPHKHGFVLSRDGVMHDISDVGIVYTPGGNFILS
                     IYTYHPVQNIWDITNPLFVNLTQATYNYFNIAAQ"
BASE COUNT          423 a          375 c          484 g          539 t
ORIGIN      
        1 gatggtcgtg ccacaagatg cacaagcacc atcttctact tcagttactg caactccgcg
       61 tttttgtttg cgaaggtctt cgtaagaggt aagtaaagaa acatcaatag gagaaagggc
      121 tgcttcacgc tcctgaccaa aacttgccat ttgtttactc agttcggctt gttcatccag
      181 caattttttg tgttcattgc caagccgcga gcgaatcact tccaattcgg cttttgcttt
      241 ttctacttca gcctcggtat tctccgattg cgacatggct tctagttgac gttcttctaa
      301 agtctcttgg tgctttttaa gagactcaat ttctttttgc aggtcttgaa gttctttcgg
      361 gttttttact gtgccaccat aaaggcttga attcgattgc tcgatcttaa ttctaagggt
      421 ctcggcttca agttcgatat ttttcaaggt tttcagggct tgctggtttt cagcactgtt
      481 tttttctaaa cgggtttgcg ccgtttttag ttctttgtcg ttttctaagg tcttttgaat
      541 ggcatccaaa cgtgttttgg cgcggtcaat ctgacggtct acttgttgaa ggcggaaaag
      601 tcccagcgat gcactcataa atcaaattat acccggcacg gtcttaaaat gccctttttt
      661 gaaatggaaa gtatataaaa acaccccgtt catgaaagaa cggggtgttt gggttattgc
      721 gccgcgatgt tgaagtaatt gtacgtcgct tgtgtgagat tgacgaacaa cgggtttgta
      781 atatcccaaa tgttttgcac cgggtgatag gtataaatcg agagaatgaa attccctccc
      841 ggagtgtaca cgatgccaac gtcactgata tcatgcatga cgccatcacg cgagagcaca
      901 aatccgtgct tgtgggggac gagcgtgcca tctggaacgc cgccctgaat gagggcacca
      961 aatttatcca acgcgaggaa gtcgatgatg agcttgcaag tgtctggagt gactttgtct
     1021 gggaaggctg cgattaacgc cccgcctcca ttttggacgc actggtagat gtctgctagc
     1081 aaggttccca tttcggacgg agttgtttgc gaataggggt ctgggtctgt gaaaatgtct
     1141 gcgcgagaat tgccaggggt gacctttcct ggaatcaagt ttggtgcacc aaggaagaac
     1201 ataccgctga tgtaggtact ggtcagcccg agttgttcca tgttttggga gacgataatc
     1261 ggaccgttat tgggatcgat acgttcaagg atggaatctg tagcagagtt gtctgagata
     1321 cggaacacac gagtaatggc ttctgtggtc gctgtatcga gattcgtgcc atgattgatc
     1381 aggtatgaag tcagaatggg caacttgatc gtactggatg cagtgaaagc gacgtctggt
     1441 tctactgtga tctcttgttt attgttgatc aaaaagtgaa tttcctgccc agtctgcaag
     1501 tccatcatgt agtaaccaat caaaccgtca aagtctgatg tggtgatgat ttgtttgagc
     1561 aggatttcaa gattttcgat ggttgggcgt gatgcggtgg tttggttgaa cgtcaataca
     1621 acagagcggt ctgcaggaga gcgcaaagcg tttgtgatga tcacaacagc acggttgaca
     1681 tcaagagtct gcccgggagc gccggggagg aaggttgtcc cgccggggac gggttgtgcg
     1741 ggggcggggg ggatatcgta ccgcgatgca atttcattct gtagaaattc tctcagacgg
     1801 tcttcggata tgcttgcact t
//
LOCUS       DAOREF010000710         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_54930_length_1817_cov_4.387628, whole genome shotgun sequence.
ACCESSION   DAOREF010000710 DAOREF010000000
VERSION     DAOREF010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54930_length_1817_cov_4.387628"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1108)
                     /locus_tag="PKI33_13320"
     CDS             complement(95..1108)
                     /locus_tag="PKI33_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNM38043.1"
                     /translation="MSKNQLHDYFDELDDLSEGAQDSARKRTSIKRESKRKEGEAKLF
                     IRAQEAAKEFKFTYKAARFEEAWLLDSLTEIYEHQWISDVLRKVKGGKEASVYLCKSG
                     TEMSVPFVAAKIYRPRSLRNLKNDAQYRVGRVDLDADGSALWKEADINAIAKRTTYGE
                     EVRHRSWIAYEFKTMEKLFEAGVDVPKPYAMEKNAIVMDFIGDANTAAPILNSVNLSA
                     HEARSLFDRVIHNIDLMLANDCIHGDLSAYNILYWNGAITLIDFPQIVLPKSNPTAWN
                     IFQRDVIRICDYFSAQGIHCDGRKIAAELWTSRGYRIGKQADPKYLDPEDPADRKLWE
                     KQQ"
     gene            complement(1355..>1817)
                     /locus_tag="PKI33_13325"
     CDS             complement(1355..>1817)
                     /locus_tag="PKI33_13325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNM38044.1"
                     /translation="QPGGRELIPGIIALLIGALISAEGNIIFKIYSVNSNPVSINALS
                     LFSAAVFLGIASFFTQETWNLPSTSAGWTALAYLIIFGSVLMFYLFVYVLTRWTASSA
                     SYSILLFPLVATVFAALLANETITLPFILGGVIVLLGVWVGAFYTKPKTGA"
BASE COUNT          484 a          420 c          490 g          423 t
ORIGIN      
        1 cgcgggcgga cgttgttcat agatcatctc ctattcaaat tgtaatagag gaaggaagaa
       61 aaaagaagcc agtgcaattg cactggcttt gtacttactg ttgtttctcc cacagtttgc
      121 ggtctgcggg gtcttcgggg tcgaggtatt tggggtcggc ttgtttccca atgcgataac
      181 cgcgggaggt ccaaagttcg gctgcgattt tacgaccatc acaatgaatg ccttgagccg
      241 agaaataatc gcagatgcga ataacatcgc gttgaaaaat gttccatgcg gttgggttgg
      301 atttcggcaa aacaatctgc ggaaagtcga tcaacgtgat ggcgccgttc cagtacaaaa
      361 tgttgtatgc ggataagtcg ccatgaatac agtcgtttgc caacatcagg tcgatgttat
      421 ggatcactct gtcgaacaag ctcctggctt catgtgcaga aagattaacc gaattcaaga
      481 tcggcgcggc tgtgtttgca tcgccaataa aatccatcac gatggcattc ttttccatgg
      541 catagggttt tgggacatcg acaccggctt cgaaaagctt ttccatggtc ttgaattcgt
      601 aggcgatcca cgaacgatgg cggacctctt caccgtaggt agtgcgtttg gcgatagcat
      661 tgatgtcggc ttctttccac aaggcagagc cgtcggcatc caagtccacg cgcccgacgc
      721 ggtattgcgc atcattcttg aggttgcgca acgaacgcgg acggtagatc ttcgcggcga
      781 cgaaaggcac actcatttcg gtgccagact tgcacaagta gaccgaggct tccttgccgc
      841 ctttgacctt tcgcaatacg tcagaaatcc attggtgttc gtagatctct gtcagcgaat
      901 ccaagagcca ggcttcttcg aagcgcgcgg ctttgtaggt gaacttgaat tctttggcgg
      961 cttcctgggc gcggatgaag agcttcgcct cgccttcttt gcgtttggac tcgcgtttaa
     1021 tagaagtacg cttgcgagcc gagtcttgtg caccttcact cagatcgtct aattcatcaa
     1081 aatagtcatg taattgattc ttactcattg ttataaaacc ttatctctta acaaaaaaac
     1141 cacagggacc ttcctgcggc tggttgaatg aggttaaaca aaaaccgcag gctgcgagcc
     1201 tgcggttgaa tcgatagggc aagactatgc caattcgctc aacggaggcg cgccaaacaa
     1261 aacaactgtg gaaaccaaga tagcttgcat gttttcgagc ctcctttcgt ggaataagat
     1321 agtgaagaaa ttttatgcca agccccaagc aacgtcaagc cccagttttg ggtttggtgt
     1381 agaaagcacc cacccacacc ccaagaagga caatgactcc gccaaggata aagggcaagg
     1441 taatggtctc gtttgccagc aaggcggcga agacagtagc caccaaggga aaaagcaaaa
     1501 tggaataaga agcggaagaa gccgtccagc gagtgaggac gtagacgaac aggtagaaca
     1561 tgagcaccga gccgaagatg atgagatagg caagggctgt ccagccggcg gaggtggagg
     1621 gcaaattcca ggtttcttgg gtgaaaaaag aggcgatgcc aagaaataca gcggcagaga
     1681 acaaggacag ggcattgatc gagacggggt tgctgttgac agaatatatc ttgaaaatga
     1741 tattcccttc ggcgctgatg agagccccaa tgagcaaggc aataatgccg ggaattagct
     1801 cacgcccgcc gggttgg
//
LOCUS       DAOREF010000711         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_54947_length_1817_cov_2.818956, whole genome shotgun sequence.
ACCESSION   DAOREF010000711 DAOREF010000000
VERSION     DAOREF010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54947_length_1817_cov_2.818956"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PKI33_13330"
     CDS             <1..407
                     /locus_tag="PKI33_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38045.1"
                     /translation="VTYDPADLSHMFLDHYDEDHQAVLMKRELDKRREEFDEHIAEKA
                     GVKKTGEKAEGVITNVEDLNLPYPAVYPSKGMRLQVNVTPNEGSTFLADIDAIISEKS
                     IEKFSVGKKVFVRFDPRNLKRVVLDTERNKKL"
     gene            575..1036
                     /locus_tag="PKI33_13335"
     CDS             575..1036
                     /locus_tag="PKI33_13335"
                     /inference="COORDINATES: protein motif:HMM:NF020859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HNM38046.1"
                     /translation="MENFDWTTTICLCGFSLLALALMMPVFIFFNKIGKKQTEEVEKY
                     QKSIRDNGGVLAPAVVVGARVLSGGRRKSGPPSFVVRFEVEVTPNNGPSFRTKLRHSI
                     TPNGYKIENYEMVSEYGKKVMIMYDPHDVSRVYIDTYDLDNYRPARTIWQF"
     gene            1218..>1817
                     /locus_tag="PKI33_13340"
     CDS             1218..>1817
                     /locus_tag="PKI33_13340"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNM38047.1"
                     /translation="MHFEFDWVSDPASWIALITLIALELVLGVDNIIFISILSGKLPK
                     EDQPRARTTGIMLAVITRIALLLSLSWIISLEEPFFYIPWFGEHGNIGISGRDLILIV
                     GGLFLLWKSTHEIHEKLEGKEGVASAKVAANFVSVIIQIMLLDIVFSLDSVITAVGMV
                     DHITIMIIAVIIAAIIMIFVAAPIGDFVEHHPTIKILALS"
BASE COUNT          513 a          323 c          400 g          581 t
ORIGIN      
        1 gggttacata tgaccctgcc gatctttcgc atatgttttt agaccattat gatgaggatc
       61 accaagctgt tttgatgaaa cgagaattgg ataaacgtcg tgaagaattt gatgaacata
      121 ttgctgaaaa agcaggtgta aaaaagaccg gtgagaaagc tgagggtgtg atcaccaacg
      181 ttgaggatct aaatctgccg taccctgcag tatacccatc aaaagggatg cgtttgcagg
      241 tcaatgtaac tccaaatgag ggctcaacat ttttagcaga tattgatgcc attatttcag
      301 aaaagtctat tgagaaattt tcggttggta agaaggtgtt tgtccgcttt gaccctcgga
      361 accttaagcg ggttgtcttg gatacagaga gaaataagaa actctgacaa tataagcaat
      421 cgcattgttt agtaattgct caaattttca tgataacttg agtggcaatg aagtttaccc
      481 agatgttcaa gatcaagtaa gcataaaatc cattgtaaaa tacacccaac cttgtttgat
      541 tgggtgtatt tatttttgtt tgaagaggat tgttgtggaa aattttgatt ggacgaccac
      601 catttgtctc tgcggttttt cgttgctcgc actcgcttta atgatgccag tttttatatt
      661 ttttaataag attggaaaaa aacaaacaga agaagtagag aaatatcaaa aatccattcg
      721 cgataacggt ggagttttag cgcctgcggt cgttgttggg gcgcgagttt tgagcggagg
      781 tagaagaaaa tcaggtccgc cctcttttgt tgttcgattc gaagtagagg ttactccgaa
      841 caacggtcct tcattccgca caaaactacg ccattcgatc acccccaatg gatacaagat
      901 cgaaaattat gaaatggtca gtgagtacgg caagaaggtg atgattatgt atgatccaca
      961 tgatgtatcc cgagtttata ttgatactta cgatcttgac aactatcgac ctgcacgaac
     1021 gatatggcaa ttttaatgtt ttgtccgctt cgacgcgtag aatcccaaat ggtttgtatt
     1081 ggatactgag aaaatgaatt catcatttaa taaataaggc ttcataatac gattatctag
     1141 atgggtactc agcaactcct cggctttttt atcgaatttg gtaaaattgc ctcaatcttt
     1201 tcgtaggagt agaagtaatg cattttgaat ttgactgggt gtccgatcca gcatcatgga
     1261 ttgcgctgat tacgctaatc gcgcttgaat tagttttagg ggtagataat attattttta
     1321 tttctattct ttctggcaaa ttgccgaaag aagatcaacc ccgtgcccgt accacgggta
     1381 ttatgctggc ggttatcaca cgtattgcat tgttgctctc tctttcatgg attatcagcc
     1441 ttgaagaacc tttcttttat attccttggt ttggtgaaca cggaaacatt ggtatttctg
     1501 gtcgtgattt gatccttatt gttggcggac ttttcttatt atggaagagt acccatgaaa
     1561 tccatgaaaa attggaaggc aaagaagggg ttgcatctgc gaaggtggct gccaactttg
     1621 tgagtgttat catccaaatt atgttgttgg atattgtctt ctcacttgac tccgtgatca
     1681 ccgcagttgg aatggttgac cacattacga tcatgatcat cgctgtaatc attgcggcaa
     1741 ttattatgat ctttgtagca gcacccattg gcgattttgt ggaacatcat cctactatta
     1801 agattttagc cttgagt
//
LOCUS       DAOREF010000712         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_55247_length_1812_cov_2.771201, whole genome shotgun sequence.
ACCESSION   DAOREF010000712 DAOREF010000000
VERSION     DAOREF010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55247_length_1812_cov_2.771201"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKI33_13345"
     CDS             complement(<1..198)
                     /locus_tag="PKI33_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38048.1"
                     /translation="MNNKKQIDKDFEYWIAWAERTKALPNLVAELRTRADETGRKEAV
                     ALLRAIHTRRRSEIRGGVIAAV"
     gene            complement(191..367)
                     /locus_tag="PKI33_13350"
     CDS             complement(191..367)
                     /locus_tag="PKI33_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38049.1"
                     /translation="MKLFIGITELIMALVALIVMFTSVHPIAKAGILLVVLFGGKGIF
                     YVYKGLSRKGEEYE"
     gene            complement(417..758)
                     /locus_tag="PKI33_13355"
     CDS             complement(417..758)
                     /locus_tag="PKI33_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38050.1"
                     /translation="MHDDFDLLMTVHYPDDLAEAFGYNSDARYVGFYWDHAGDELTWL
                     DGRRSLCGAECQYFLKFASLHQINKHRIGDSETLPTNYLLVDRKSNNLYVGDKRLVEE
                     FLKLQWSPIQS"
     gene            complement(765..1079)
                     /locus_tag="PKI33_13360"
     CDS             complement(765..1079)
                     /locus_tag="PKI33_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38051.1"
                     /translation="MTGPNYVLLIILALIAVLGDIGAIEMYFSVNMYSLPLIAVWIVL
                     FTSRLRTTMNWTTENFFPLAIGCALIYTSSNVIHNPALLPSVILTFGGATFFAAQKMQ
                     EG"
     gene            1401..>1812
                     /locus_tag="PKI33_13365"
     CDS             1401..>1812
                     /locus_tag="PKI33_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38052.1"
                     /translation="MRKISKFVLQVTLLGFEMVLLFAFAAIAKPSLDTNIVVRTGLYD
                     SRSGARQHSYNLSWFVDTQQVSLPPASNLTNAAKGLFNSRLDIGGNGYTSYPIAAKGS
                     PLEVTFTVNFKNAVSIPVHFAMPNMEGIVVASKTI"
BASE COUNT          465 a          420 c          407 g          520 t
ORIGIN      
        1 cactgctgcg ataacgcctc ctcgtatttc tgatcgacga cgtgtatgga ttgcccgtaa
       61 taatgccaca gcttccttcc gtccggtttc atctgcgcgt gttctcaatt ctgcaacaag
      121 gttcgggagt gctttggtac gttctgccca ggcaatccaa tattcaaagt ctttatcaat
      181 ctgtttctta ttattcatat tcttctccct tcctggacag ccccttgtac acatagaata
      241 tacctttacc gccaaacagc acgacaagca aaatgcctgc ttttgcaatg gggtggacac
      301 tggtgaacat aacgatcagc gcaacgagtg ccatgatcaa ttctgtaatt ccgatgaata
      361 gtttcatagt tttcattggt tttccttaat aaagtatatt tcgcatagac attgtcttat
      421 gattgtattg gactccattg caatttcagg aattcttcaa ccaacctttt atcaccgaca
      481 taaagattat tagatttccg atcgacgagc aggtagttag tgggcaaggt ctcactgtca
      541 ccgatccgat gtttattgat ttgatgcagc gaggcgaact tgagaaagta ttgacactcg
      601 gctccgcaca aggagcgccg tccatcgagc caggtcaatt catctcctgc atggtcccag
      661 tagaaaccaa cgtagcgggc gtcactgttg tatccgaaag cttctgctag gtcatcaggg
      721 taatgaacag tcatcagcaa gtcaaagtca tcatgcatga ttcattaccc ttcctgcatc
      781 ttttgcgcgg caaagaatgt ggcgccgcca aaggttagga ttacacttgg aagcagcgcg
      841 gggttgtgta tgacattgct gctggtgtaa atcaaggcgc agccgatggc gagagggaag
      901 aaattttccg tggtccaatt catggttgtg cgtaaacggg atgtgaacaa aacgatccag
      961 acggcaatca atggcagcga atacatattc acagagaagt acatctcaat cgcgccgatg
     1021 tcgcccagaa ctgcaatgag cgcgagaatg atcagcaaaa cgtagttggg tccagtcatc
     1081 atctgcatcc tcgcataaaa ccgtaaagac ggtaggtgtt ggtaggggta ttcataaata
     1141 gtgggttcct tttcgtaacc gagttatacg aagtaaattt acatgcttat tgggtcattt
     1201 aaactcggtt tatggcttaa atatacggtt ttacttcgta caagtcaagt tgtacgaagt
     1261 aaatttgcac gatttttgag gctcccccgc agccttcttt tcgatttttt atcccaccaa
     1321 ctgaccgaac cgaactccat tgtgagtctc aggagcctgc tcctgtctct gaaatttcat
     1381 ttcaccttgg aggtatcgca atgcgtaaga tctcgaagtt cgttttgcaa gtcacgctgc
     1441 tcggatttga aatggtgttg ctgttcgctt ttgctgcgat tgcaaagcca tcgttggata
     1501 cgaatattgt tgttcgtaca ggactatatg actcgcgttc tggcgctcgt caacattcct
     1561 acaatctgag ttggtttgtg gacacgcaac aggtatcact cccaccagca tcgaacctga
     1621 caaacgctgc gaagggactt ttcaattcgc gactggacat cggcggtaat ggttacacct
     1681 catatcccat cgcagcaaaa ggttctccgc ttgaagtgac ttttacggtc aatttcaaga
     1741 acgcagtctc tataccggtt catttcgcga tgcccaatat ggaaggtatt gttgtggcaa
     1801 gcaaaaccat tt
//
LOCUS       DAOREF010000713         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_55418_length_1809_cov_3.545040, whole genome shotgun sequence.
ACCESSION   DAOREF010000713 DAOREF010000000
VERSION     DAOREF010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55418_length_1809_cov_3.545040"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKI33_13370"
     CDS             <1..374
                     /locus_tag="PKI33_13370"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM38053.1"
                     /translation="AAFTFTPTVEAGPCTNQLTFLDDLTVEDGTAFTPGAAIDKQWLV
                     QNDGTCDWDATYKLKWVGGYTLGVTEEQPIFPARAGVQVTLRIVFTAPAESGTYQTTW
                     QAIDPNGNLFGDTVYMEIIVQ"
     gene            371..1120
                     /locus_tag="PKI33_13375"
     CDS             371..1120
                     /locus_tag="PKI33_13375"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HNM38054.1"
                     /translation="MKLTRYLIACSFVLFLLSCGPVRCGDDENCLRVLFVGNSYTYVN
                     DLPTMFSELSRSGGHKVEVSIVAEGGFTLAGHANSAKFVEALSSSQWDYVVLQEQSQI
                     PSVEYSRVNMMYPAARNLVQQIRTVNAQPLFFSTWAHRDGYPEKGMTNYEAMQYQIDQ
                     GYLRIARELNVQVVHVGVAWFRALNKYPSLQLWQEDGSHPSEQGTYLAACVFYITLFK
                     ESPVGLNYHANLTEEVASQLQSVAESILTNP"
     gene            1144..1512
                     /locus_tag="PKI33_13380"
     CDS             1144..1512
                     /locus_tag="PKI33_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38055.1"
                     /translation="MPTASEFPIVFESLRDILKPYAKKLTLKKDTSGLYYLDAGYSEK
                     WKRDLFFASVQIKKSYVSFYLMPVYMYPDLLKNISPELKKHMQGKSCFNFKKIEKSLF
                     EELSQLTKQGHERMMKEEFS"
     gene            complement(1552..>1809)
                     /locus_tag="PKI33_13385"
     CDS             complement(1552..>1809)
                     /locus_tag="PKI33_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38056.1"
                     /translation="RVVMIEGLVISVISWAISIPCSVPLSIFLSNSLGLSLLARPLDY
                     IFSVPAMLIWLALTVVIAIIASILPAQSAARLTIRDALVYE"
BASE COUNT          498 a          412 c          442 g          457 t
ORIGIN      
        1 ccgcagcgtt cactttcact cccaccgtgg aagcaggtcc ctgcacaaat caactaacct
       61 ttctcgatga tcttactgtc gaagacggca cagctttcac tcccggtgcc gctatagata
      121 aacagtggct tgttcaaaat gatggtacct gtgactggga tgcaacctac aagttgaagt
      181 gggtgggcgg gtacaccttg ggtgttaccg aagaacaacc aatttttcca gcacgcgcag
      241 gtgtacaagt gacattgcgt attgtcttca cagcgccggc agaatcaggc acctaccaaa
      301 ccacttggca agctattgac cctaatggca atctttttgg tgataccgta tacatggaaa
      361 tcattgtgca gtgaaactta cacgatactt aattgcatgt agttttgtac tattcctcct
      421 cagctgcgga cctgtccgct gtggagatga tgaaaactgc ctgcgtgttt tatttgtagg
      481 gaatagttac acctatgtca acgacttgcc aacgatgttc tccgaactgt ctcgttcagg
      541 cgggcataaa gtggaagtaa gcattgtagc cgaaggcggt tttacattag caggccatgc
      601 caattctgca aagtttgttg aagccttaag ctccagtcag tgggactatg ttgtgctgca
      661 agagcaaagc cagattcctt ctgttgaata ttcacgtgtg aatatgatgt accccgctgc
      721 ccggaatcta gtgcagcaga ttcgaactgt aaatgcccaa ccgcttttct ttagtacatg
      781 ggcacaccgt gacggttatc cagaaaaggg catgaccaat tatgaagcca tgcaatatca
      841 aattgaccaa gggtatttac gaattgcacg tgagcttaac gtacaggtcg tgcatgtggg
      901 agtggcatgg tttcgggcgc tcaataaata tcccagccta cagctttggc aggaggacgg
      961 cagccatccc tctgaacagg gcacttatct cgctgcttgt gttttctata tcaccctttt
     1021 taaagaaagc ccggtgggtt tgaattacca tgcgaacctt acagaagaag tcgctagtca
     1081 attgcaatct gtggctgaaa gtattctgac caacccataa agtagaaata aaggagagaa
     1141 ctcatgccta cagcatcaga attcccgatt gtgttcgaaa gtttgcgtga cattcttaag
     1201 ccttatgcca aaaagctgac cctcaagaag gatacaagtg gtctgtacta tctggatgca
     1261 ggctatagcg aaaagtggaa gcgcgacctt ttcttcgcct cggtgcaaat caagaagagt
     1321 tacgtctcgt tctatcttat gccagtttat atgtaccccg atttgttgaa gaacatctcg
     1381 cctgagctca agaaacacat gcagggaaaa tcctgtttca atttcaagaa aatcgaaaaa
     1441 tccctgttcg aggagttaag tcaactcacc aaacagggac atgaacgcat gatgaaagaa
     1501 gaattttcgt aattgtttta gggacgatat gcttgttgct gtccctgaac tttattcata
     1561 caccagggcg tctcgaattg taaggcgggc ggcgctttgc gcaggcagga tgctggcaat
     1621 gatggcgatc accacggtta gggcgagcca gatcagcatg gcagggacgg agaagatgta
     1681 atccaatggg cgggcaagca gcgaaagccc gagggaattg ctcaaaaata tagagagggg
     1741 aactgagcag ggtatggaga tcgcccaact gatgacactg atcaccaagc cttcgatcat
     1801 cacgacccg
//
LOCUS       DAOREF010000714         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_55635_length_1805_cov_5.005143, whole genome shotgun sequence.
ACCESSION   DAOREF010000714 DAOREF010000000
VERSION     DAOREF010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55635_length_1805_cov_5.005143"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..347)
                     /locus_tag="PKI33_13390"
     CDS             complement(102..347)
                     /locus_tag="PKI33_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNM38057.1"
                     /translation="MSVSESAVRESQVRQLLENLSAYIEQFHGGTVEFISLAGNDLQI
                     RLGGACLNCPLLPATLHGWVEGTVHQFFPEIHVVASN"
     gene            complement(347..805)
                     /locus_tag="PKI33_13395"
     CDS             complement(347..805)
                     /locus_tag="PKI33_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNM38058.1"
                     /translation="MLDNLPGIPLFENLNPEQRFTLMSVFEKFACSPGTVIFEQGDIA
                     KYLYLILRGKAVISYKPYDGPRIVLSRLKDGDVFGWSAVVGGKKYSSSVASETTLETI
                     RIRRELLQNLLTQTPETGKIIIDRLALNVSPRWKNAHEQIKPLIHEERKQ"
     gene            959..1162
                     /locus_tag="PKI33_13400"
     CDS             959..1162
                     /locus_tag="PKI33_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38059.1"
                     /translation="MKKTKNLSSFEKLLLGLEKPEILEVDVPLRKGSACPNCNEGVLD
                     YNGMLQLECPSCGFVNGESGGCT"
     gene            1162..1788
                     /locus_tag="PKI33_13405"
     CDS             1162..1788
                     /locus_tag="PKI33_13405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNM38060.1"
                     /translation="MTEINSREETNELDIMEQEIPANTTPLGRIMALVMIGFGFLALG
                     IAFLLLKNQDEVSTGSSIDFSTVPAKVDFPAPDLSFIDLGTDPVSLSDYRGSVVLVNL
                     WATWCPPCREEMPTLQAFYEEYKSEGFVLIAIDQGETLPQVLPFVKELKLTFPIWLDY
                     TSDAGRAFETMNLPSSYVIDRTGQVRLMWIGGISKKNLEKYVPDVIEE"
BASE COUNT          489 a          383 c          396 g          537 t
ORIGIN      
        1 gatccttata ggcggttttc tcgtcgttcg caatttcacc cgacatgctt agctgctaaa
       61 agaatcaaaa agccctcgca atttgcgagg gctttatttt tttaattact tgctactaca
      121 tggatttctg gaaagaactg atgaactgtt ccttccaccc agccatgtaa ggttgctgga
      181 agcaatggac agttcaagca ggctcctcct aaacggatct ggaggtcatt ccctgcaagc
      241 gatatgaatt ccacagtccc gccgtggaat tgctcaatgt aggcgctcaa attttccaac
      301 aactggcgta cctgactttc acgaacggct gactcagaaa cagacattat tgcttccttt
      361 cttcgtgaat gagcggttta atttgttcgt gggcattttt ccaacgagga gagacattca
      421 atgccaatcg atctatgatg atctttcccg tttccggcgt ttgtgtgagc aggttttgta
      481 gcaattcgcg acggatacga attgtttcta gagttgtttc gctggctaca ctggaagaat
      541 attttttccc gccaactacg gcagaccacc caaatacatc cccatcttta aggcgcgaaa
      601 gcacaatccg cggaccatca taaggcttat agctaatgac tgctttaccc cttaatatca
      661 ggtataaata tttggctata tcgccttgtt caaagatgac cgtcccaggg gaacacgcaa
      721 atttctcaaa caccgacatt aaagtaaacc tttgttcggg gttgagattt tcaaataacg
      781 ggattccggg caggttatct agcatttgaa atcatgttaa ccattgtata aataccctac
      841 cagtatcatc catcctaaac cggatgacat tcatcctgtt ttctatttgg gtattttccc
      901 ttagtgtata ctttgtgaaa acggtaattt ttacttatat tagaccttca gaatcttgat
      961 gaaaaaaaca aaaaaccttt cgtcttttga aaaactatta cttgggttgg aaaagccaga
     1021 aattctcgaa gtggatgtgc cgcttcgcaa aggctcggct tgcccgaatt gtaatgaagg
     1081 ggtattggat tacaacggta tgcttcaatt ggaatgcccc tcctgtggct ttgttaacgg
     1141 ggagagtgga gggtgcacct gatgaccgaa ataaactccc gtgaggagac taacgaattg
     1201 gatattatgg aacaagaaat acctgcaaac acaacccctc ttgggcgtat tatggcattg
     1261 gtgatgatcg ggtttggatt cctcgcacta ggaatcgctt ttttgctgtt gaagaatcag
     1321 gatgaagtat ccaccggctc ttccatcgac ttttcaacgg tgcctgcaaa agtagatttc
     1381 cctgccccag acctgagttt tattgacttg ggcactgacc ccgtgtctct ttcagattat
     1441 cgtggaagtg ttgtattagt aaatctgtgg gcaacatggt gcccaccttg ccgtgaagag
     1501 atgccgactc tacaagcatt ttatgaagaa tataaatctg aaggttttgt tttgattgct
     1561 atcgatcaag gtgagacgtt gcctcaagtg cttccttttg tgaaagaact caagttaacg
     1621 ttcccaatat ggttagacta taccagtgat gccggacgtg cattcgaaac catgaatttg
     1681 cccagctcct atgttattga ccgtactggt caagtgcgct tgatgtggat tgggggcatt
     1741 tcaaaaaaga atctagaaaa atatgttcct gatgtgattg aggagtaaaa ataaaatgga
     1801 tgtga
//
LOCUS       DAOREF010000715         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_55640_length_1805_cov_4.193714, whole genome shotgun sequence.
ACCESSION   DAOREF010000715 DAOREF010000000
VERSION     DAOREF010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55640_length_1805_cov_4.193714"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKI33_13410"
     CDS             complement(<1..258)
                     /locus_tag="PKI33_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38061.1"
                     /translation="MTETNPSSEQNPQVTNLSQVDVETVNAQLVRMHQSSAGLVTSED
                     VDMTMSAVAQAKVTNLKLTDSGVAALHADTATVQNSVVGVAQ"
     gene            complement(310..951)
                     /locus_tag="PKI33_13415"
     CDS             complement(310..951)
                     /locus_tag="PKI33_13415"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNM38062.1"
                     /translation="MHIPKYYREEDQDKIVEFLKANSFPAIVSSDGEKLLATHLPVEV
                     IENADGSLTVLGHMSRANPQWKSFGEQEVLLIFQGAHTYISPRWYNHVNVPTWNYINV
                     HVYGKVRMLEGEELHSLLSKLVSKHETGTGYSMEGLPADFVEKEMRGVAGFALDVTRL
                     DAASKLSQNRDDESYANIISHLEARGDDISAEIAHEMRDRRPSLTNPPQTDKI"
     gene            1013..1147
                     /locus_tag="PKI33_13420"
     CDS             1013..1147
                     /locus_tag="PKI33_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38063.1"
                     /translation="MSKRYKKKRSTTPAWVDSLIVILEILGAIGTVIAGIYSLLTWVF
                     "
     gene            1205..1696
                     /locus_tag="PKI33_13425"
     CDS             1205..1696
                     /locus_tag="PKI33_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38064.1"
                     /translation="MEQKTENKSVNRIGLLFLILGGVFAFISCLILVAGITLDLFGTE
                     TRGKMTNISYDAKRSDNPFTAQVTFTTANGEEKSFISWEDRFYFELDERIQRSGSTAP
                     FEGVKVRYLEAFPALAKVVFVYHAEYVNQIVWLFWSGVALMIGIISRRNKPITIDLSQ
                     RNK"
BASE COUNT          462 a          417 c          431 g          495 t
ORIGIN      
        1 ttgagcgact cctactactg aattttgaac ggtagcagtg tcggcatgca gagccgctac
       61 gccagagtcg gtcaacttta agttggtgac cttcgcctgc gctaccgcac tcatggtcat
      121 atcgacatct tctgaagtga ccaaaccagc cgagctttgg tgcatgcgaa ccaactgcgc
      181 gttgaccgtt tccacgtcca cttgggagag gttggtcacc tgcgggtttt gctcagaaga
      241 aggatttgtc tcggtcatga ttttggcaga aaatacgcct cctcacggag gcgcaggttg
      301 aataacggct cagattttat ccgtttgagg ggggttcgtc aaggaagggc gcctatcccg
      361 catttcgtgc gcaatctcgg ctgaaatatc atctccgcgg gcttcgaggt ggctgataat
      421 gtttgcatag gactcatcgt cgcggttttg gctcagtttc gaggcggcgt cgaggcgggt
      481 tacatccaaa gcaaaacctg ccaccccacg catctctttt tcaacgaagt cggcgggcaa
      541 gccttccatg ctatagcctg tgccggtttc gtgcttagag accaacttgc taagcaggga
      601 atgtaattcc tccccttcca acatgcggac ctttccatac acatgcacat tgatgtaatt
      661 ccaggttggc acattgacat ggttatacca gcgcggagaa atataagtgt gcgcaccctg
      721 aaaaataagc agaacttcct gctcgccaaa agacttccac tgcgggttgg cgcgagacat
      781 atgccccaga acggtcagag acccatcggc attttctata acttcaaccg gtaaatgcgt
      841 ggcaagtaat ttttcgccat cgctcgaaac aatcgccgga aaactattgg cttttagaaa
      901 ttccacgatc ttatcttggt cttcttcacg ataatatttt gggatatgca tgttaaacct
      961 ctaaggaaag tataacaata tttcttaaga ggctcaggta gaattttgcg acatgtcgaa
     1021 acgatataaa aagaaacggt ctactactcc agcttgggta gacagcctca ttgtcatttt
     1081 agaaatcctt ggagctattg gcactgtgat cgcaggcatt tatagcctgc ttacgtgggt
     1141 gttttgataa accttggtta taatctgaac cttggtcata aaattttaga ggtaggagac
     1201 cctcgtggaa caaaagacag aaaataaaag cgttaaccga attggtttac tattcctaat
     1261 tctcggaggt gtttttgctt ttattagttg cctcatattg gttgctggaa ttactcttga
     1321 tcttttcgga accgaaacca gagggaaaat gaccaacata tcatacgatg ccaaaaggtc
     1381 tgataacccc tttacagcgc aagtcacatt cacgacagca aacggcgagg aaaaatcttt
     1441 tatctcttgg gaagaccgtt tctattttga gctggatgag cgcattcaga gaagcggtag
     1501 tacggcacca tttgagggcg taaaagttcg ttatcttgaa gccttccctg ctctagcaaa
     1561 ggtagtcttt gtgtaccatg ccgaatatgt gaaccagatc gtttggcttt tctggtctgg
     1621 tgttgcgttg atgatcggga tcatttcccg tcgtaataag ccaatcacaa ttgatctcag
     1681 tcaaaggaat aagtaatcat gctcaaaaaa ctacgtgaac ctgtcaacag cctcaccaac
     1741 tggggcggtg cggttcttgc cttgatcggc ttgatcgcat tgctcatcgt tgggtggagc
     1801 acgcc
//
LOCUS       DAOREF010000716         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_55968_length_1800_cov_3.839542, whole genome shotgun sequence.
ACCESSION   DAOREF010000716 DAOREF010000000
VERSION     DAOREF010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55968_length_1800_cov_3.839542"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKI33_13430"
     CDS             complement(<1..932)
                     /locus_tag="PKI33_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNM38065.1"
                     /translation="MQTLIDFGVSFIIALQRLGEWQIPIMAFFSRLGTEEFFFIVLPL
                     LFWNIDSALGIRVAFILAVSNFINLIGKVGFAGPRPYWVSSHIQPWWPETSFGIPSGH
                     AQHAMSVWGIIALYKKEKWITIVCVILIFMIGFSRLVLGAHFPHDVILGWLLGAFLLW
                     LYVRYWDATVTWLSNMNFSKQVGVAFLVSMIFVVIGMIVTNTRSDFQIPESWMTNALR
                     SGIAPDPIDPNGVFTAVGTFFGLAVGAAWTHLRGGYQVDGPVWKRVLRYVIGLAGVLI
                     LWQGLGAIFPRGDGLVVYSLRFVRYSLVGWWISGG"
     gene            1004..>1800
                     /locus_tag="PKI33_13435"
     CDS             1004..>1800
                     /locus_tag="PKI33_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38066.1"
                     /translation="MEKTSVRNWDWLSNLIMLFAVLFSAWRLQTADWTEGLGQVRNVA
                     VLGFALGLGVGYSQFKRKPIFFLVAGYTVAILIWQLLGTIPFNKDQTYLLDKFSILFG
                     RMFTSLGELFAGRAVEDQFFVVALLCIPYWFASLYSGFQLTRHSNFLGTILPNGVLTL
                     LIHLYHYTNQDYTWMLGVYLFLALLLLSRLKYLADRRKWSKERIQVQSDSGLDITNTT
                     VAVATVLILLAWGSPYILPATTEGKQIWREVYGQIFPKDRFKNVFASV"
BASE COUNT          542 a          409 c          396 g          453 t
ORIGIN      
        1 ccaccagaaa tccaccaccc taccaaggag taacggacaa agcgaagaga atacaccacc
       61 aaaccatcac cccgaggaaa aatagcacca agcccttgcc acaaaatgag aactccagcc
      121 aaaccgatga catagcgaag tacacgcttc caaaccggac cgtctacctg atagccacct
      181 cgaaggtgag tccacgctgc acctactgcc agcccgaaga aggtcccaac tgcagtgaag
      241 actccgttcg ggtcgatagg atcaggcgcg ataccggagc gaagcgcgtt cgtcatccat
      301 gattctggga tttggaaatc acttctcgta tttgtaacaa tcatcccaat aaccacaaaa
      361 atcatagaaa ctaaaaaagc aactcccact tgctttgaaa agttcatatt ggaaagccag
      421 gtaacggttg catcccaata gcggacatat agccacaaca aaaaggcgcc taacaaccaa
      481 ccaaggatga catcatgcgg aaaatgtgcg ccaagaacta aacgcgaaaa gccgatcatg
      541 aaaataagaa tgacacagac aatcgtgatc catttttctt ttttgtacaa agcaatgatg
      601 ccccaaacgc tcatggcatg ttgtgcatgc cctgaaggga tgccgaacga ggtttcgggc
      661 caccaaggtt gaatatgtga gctgacccaa tatgggcgcg gaccagcaaa gccaactttg
      721 ccaataagat taataaaatt gctcacagcc aaaataaaag ccactcgaat gccaagtgct
      781 gagtcaatgt tccaaaaaag gagcggcaaa acaataaaga aaaattcttc tgtgcctaat
      841 cggctgaaga aagccatgat gggaatttgc cattcgccca accgttgaag tgcaattatg
      901 aaagacacac caaagtcaat aagggtctgc atctagtcct ccagtagggt ttttcttaga
      961 aagtgaattg acgtaacaat gcgacccatt gtacactcaa agaatggaaa agacatcggt
     1021 gcgtaattgg gattggctct ccaatctgat tatgcttttt gcagtgttat tttctgcctg
     1081 gcgcttacaa accgcagact ggactgaagg cttggggcaa gttcgcaatg tggcggtact
     1141 tggttttgcg cttgggctcg gggtaggata cagccaattt aagcgaaagc ctatattttt
     1201 tctggttgca gggtatacag ttgctatttt gatatggcag ttattgggga caattccatt
     1261 caataaagac caaacttatt tattagataa attctcgatc ttgtttggaa gaatgtttac
     1321 atctcttggt gaactttttg ctggtagagc tgtggaagat cagttctttg ttgtcgcttt
     1381 attgtgcatc ccttactggt ttgccagcct ttacagtggg tttcagttaa ccaggcattc
     1441 caactttctg ggcaccatcc ttccaaatgg tgtgttgacg ttgctcatcc atctctatca
     1501 ctataccaac caagattaca cttggatgct tggggtttat cttttccttg cattattact
     1561 actcagccgg ttgaaatacc ttgcagaccg tagaaaatgg tcaaaggaaa gaattcaggt
     1621 tcaaagtgat agtgggcttg atatcacaaa tacaaccgtg gctgttgcca ccgtcttgat
     1681 tttattggca tgggggtcgc catatattct gccagcgaca acagaaggaa agcaaatttg
     1741 gcgcgaagtg tacgggcaga tctttccgaa agatcgcttc aaaaatgtat ttgcttctgt
//
LOCUS       DAOREF010000717         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56062_length_1799_cov_2.252867, whole genome shotgun sequence.
ACCESSION   DAOREF010000717 DAOREF010000000
VERSION     DAOREF010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56062_length_1799_cov_2.252867"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKI33_13440"
     CDS             <1..847
                     /locus_tag="PKI33_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNM38067.1"
                     /translation="LLSLREGLEAALIIGIVLGALQKIRRTDLAPALWMGTLAALGVS
                     ILTAVILTLFGLSLEDGAEQIYEGITMLLAAGILTWMIFWMSGQAKNIKTELEDGINK
                     AVATTGKRAVFGLAFLAVVREGVELALFITAAFFAGNSENVTTNIILTLTGVVLGLGT
                     AVLLGWSLLAATARLNLRRFFQITGYLLILFAAGLVAHGIHEFNEVGWIPSIIEHVWD
                     VNAVVIETSVVGELLKTLFGYNGNPSLTEVIGYFVYLAVAVFFFTRTTTTQPVAKKNP
                     VQSPA"
     gene            944..1609
                     /locus_tag="PKI33_13445"
     CDS             944..1609
                     /locus_tag="PKI33_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNM38068.1"
                     /translation="MNTKYEGFEAPGITVDLVLFTVNENRLKVMLVKRAEEPFRNDWS
                     LPGGFIKKGESLDDAAQRVLVEKTTVSDVYMEQLYTFGTPKRDPRSRVITVAYIALIP
                     WQSLPQPESDKITDLTWLPVDHLPKLAFDHNEIIHYAATRLRAKVSYSNIVYGLMPKQ
                     FRLSELQSMYECIINDKLDKRNFRKRMLATGLLKETGKKDIAGAHRPAMLYQFKTKEI
                     VFL"
     gene            1619..>1799
                     /locus_tag="PKI33_13450"
     CDS             1619..>1799
                     /locus_tag="PKI33_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38069.1"
                     /translation="MTQTPIPDFFDASRVGEIWKVDYAARADQARTFALQHNLKPAST
                     SSERISLMLIDVQNTF"
BASE COUNT          443 a          469 c          425 g          462 t
ORIGIN      
        1 tctactctcc ctgcgcgaag gcttagaagc cgctctaatc attggaatcg tgttgggtgc
       61 cctacaaaaa atccgccgta ctgatctggc tcccgctctt tggatgggaa ctttagccgc
      121 tttaggcgtt agcatcctca cagctgtgat tctcactctg tttggcttga gccttgaaga
      181 tggcgccgag cagatctatg aaggtatcac catgctgctc gcggctggca tccttacctg
      241 gatgatcttc tggatgagcg gacaggcaaa gaatatcaag accgagttgg aagatggcat
      301 caataaagcg gttgccacca cgggcaagcg tgccgtcttc gggttggcgt ttcttgcggt
      361 ggtacgtgaa ggcgtggaat tggcgctctt catcaccgcg gctttcttcg cgggcaatag
      421 cgaaaatgta acgaccaata tcatcttgac cctcacgggt gtggtgcttg gtttgggaac
      481 ggctgtgttg ctaggctggt cattactggc tgccactgcc cgccttaatc tgcgccgctt
      541 tttccagatc accggttatt tgctcatcct tttcgcggcg ggacttgtcg cacatggtat
      601 tcatgaattc aacgaagtcg gttggattcc ctccatcatt gaacatgtat gggatgtcaa
      661 cgcagtggtg attgaaactt ccgtggtggg tgagttgctc aagaccttgt tcggttataa
      721 cggcaacccc tctctcacag aagtgatcgg ttactttgtc tatcttgccg tggcggtttt
      781 ctttttcacc cgcacgacca caactcaacc ggttgcaaag aaaaacccgg ttcaatctcc
      841 cgcataacaa gaaaagaccc aactaataaa aggcttcgca tttcaaaatg cgaagccttt
      901 ttcgtcttga cagaacgaat aaaatcatta tactaagtgt ataatgaaca ctaagtatga
      961 gggttttgag gcacccggca tcaccgttga cctggtcttg ttcacggtca acgagaatag
     1021 acttaaagtg atgttggtca aacgcgcaga agaaccgttc cgtaatgatt ggtctttgcc
     1081 gggcggcttt atcaaaaaag gcgagtcgct ggatgatgcc gctcaacgcg ttctcgttga
     1141 aaaaacaacg gtcagtgatg tgtacatgga gcagctttac accttcggta cacccaaacg
     1201 tgatccgcgt tcacgcgtca ttacagtggc gtatatcgcg ctcattccct ggcaaagttt
     1261 gccgcaaccc gaatcagaca aaatcaccga cctcacctgg ctgcccgtcg accatcttcc
     1321 caaacttgca ttcgaccaca atgagatcat tcattatgct gccacacgtt tgcgcgcgaa
     1381 ggtcagttac agcaacatcg tctatggttt gatgcccaaa caattccgtc tctctgaatt
     1441 gcagagcatg tacgaatgca tcatcaacga caaactcgat aaacgcaatt tccgtaaacg
     1501 tatgttggcg accggtctgc tcaaagagac cggcaaaaaa gatatcgctg gggcgcatcg
     1561 ccctgccatg ttgtatcagt tcaaaacaaa agagatcgtt tttctatagg tgaatctcat
     1621 gacccaaact ccaatccctg acttttttga cgcatcccgc gtaggcgaaa tttggaaagt
     1681 agattacgcc gcccgcgcag accaagcccg gacattcgcg cttcaacaca acctcaagcc
     1741 tgcctcaact tccagtgagc gaatctccct gatgctgatc gacgtgcaaa atacgttct
//
LOCUS       DAOREF010000718         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56218_length_1796_cov_4.485353, whole genome shotgun sequence.
ACCESSION   DAOREF010000718 DAOREF010000000
VERSION     DAOREF010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56218_length_1796_cov_4.485353"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="PKI33_13455"
     CDS             <1..1581
                     /locus_tag="PKI33_13455"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNM38070.1"
                     /translation="QIIEQWAQTRPADLPDDLSLVSQAGPVINHAPPADFVVGLKGFQ
                     PDLRTATPNIQSLVSALDVVSAQTPRIGVKRAILFITPQMTDPNLAASVEPLIQRALE
                     NKIRVFIWYVDANTTFTTTSAAVFNNLAIQTGGTMFQFSGEERFPDPEEYFSPLRRIY
                     NFSYTSRVNTSGNHTVAVQVNLPSVGPLNSETLPFTLNIQPPNAFPVAPSIQITRQAP
                     EEDPFNTEVLLPETQEIEIIIEFPDGFDRPLARTTLIVDGIAVDENTAEPFTKFTWDL
                     KPYTISGEHQLTVEAVDSLGLSKTSMPLPVVVTVIKPPSGPAAFLAKYRMPLTFGAII
                     LAGLVLFAILLSGRLRIRSLRVMQEERRVQNDPLTQSLEAFTEPAPVAVKEKTKKPRT
                     KSTKAKAAKTLENEALASLVRLQADGQVAAVPPIQLNDAEIVFGTDPVQCSQILDDPS
                     ISSVHARIRATEDGGYLLMDAQSLAGTWVNYEPIPREGYRLLHGDMVNFGQLKYRFML
                     RTPPPPRTPRITPVTPED"
     gene            1581..>1796
                     /locus_tag="PKI33_13460"
     CDS             1581..>1796
                     /locus_tag="PKI33_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38071.1"
                     /translation="MIRTTLAHLHIAALSHAGISGKNNEDRYAVSSFQLSKDDPRPST
                     FAVVADGIGGHRAGEVAAELAVNYISMH"
BASE COUNT          429 a          540 c          416 g          411 t
ORIGIN      
        1 cagatcattg agcagtgggc acaaacccgc ccagcagacc tgcccgatga cctaagcctt
       61 gtctcgcaag cgggtccggt catcaaccat gccccgccag cagatttcgt agtcggctta
      121 aaaggcttcc agccagatct gcgcaccgct actcccaaca ttcaatctct ggtcagcgcg
      181 ctagatgtgg tcagcgccca aacaccgcgc atcggtgtga agcgtgcgat tctattcatc
      241 acaccgcaaa tgacggaccc caacctcgcc gcatcggttg agccgctgat ccaacgcgcc
      301 cttgaaaata aaattcgcgt cttcatctgg tatgtggacg caaacacaac cttcacaacc
      361 accagtgctg ccgtcttcaa caaccttgct atccaaaccg gcggcaccat gttccaattc
      421 tcaggcgagg aacgcttccc agaccctgaa gaatatttct ctccgttgcg tagaatttac
      481 aatttctctt acacctcgcg tgtcaacacc tctggcaacc atactgtagc ggttcaagta
      541 aatctgcctt ctgttggtcc gctgaattca gagacacttc ctttcaccct caacatccaa
      601 ccacccaatg ccttcccggt cgcgccgtcg atacagatta cgcgccaagc gcctgaagaa
      661 gaccccttca acaccgaagt ccttttgcca gaaacacaag agatcgaaat catcatcgaa
      721 tttccagatg ggtttgatcg cccgcttgct cgcaccactc tgatcgtgga tggcattgcc
      781 gtggatgaaa acacagccga gccatttaca aaatttacct gggacttgaa gccttacaca
      841 attagcggtg agcatcaatt gaccgttgaa gctgtggatt ctctgggtct cagtaagacc
      901 agcatgcctt tgccggtagt ggtaaccgtc atcaaaccgc ccagcggacc tgctgccttc
      961 cttgcaaaat atcgcatgcc attaaccttc ggcgcgatca ttctcgctgg gcttgtctta
     1021 ttcgccatct tgctcagtgg acggttacgt atccgttcgt tgcgggttat gcaagaagaa
     1081 cgccgcgttc aaaacgaccc attgacccag tctcttgaag cgtttacaga accggctccg
     1141 gtggcggtga aagaaaaaac taaaaagcca cgcaccaagt ccacgaaggc gaaagcagcc
     1201 aaaacgttgg agaatgaggc gttggcttct ctcgtccgcc tccaagccga tggacaagtg
     1261 gcggctgtcc cgcctatcca attaaacgat gcagagatcg tttttggcac cgaccctgtg
     1321 caatgctcgc aaattttgga tgacccctct atttcgtctg tccatgcgcg gattcgcgcc
     1381 acagaagatg gcggttatct actgatggat gcccagtcgc tcgccggcac ctgggtcaat
     1441 tacgagccta tcccccgtga gggctaccgg ttactgcatg gggatatggt aaactttggt
     1501 caattaaagt accgctttat gttgcggact cccccgccac cgagaactcc aagaatcacc
     1561 cctgtgactc ctgaagacta atgatccgta ccacacttgc tcatttgcac atagccgctc
     1621 ttagccatgc gggcatctct ggcaagaaca atgaggaccg gtatgccgtc tcgtcgttcc
     1681 agctcagcaa agatgaccca cgcccttcaa cgtttgccgt tgttgcggat ggtattggcg
     1741 gacaccgcgc gggtgaagtc gccgcggaat tggcagttaa ctacatcagc atgcat
//
LOCUS       DAOREF010000719         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56220_length_1796_cov_4.295807, whole genome shotgun sequence.
ACCESSION   DAOREF010000719 DAOREF010000000
VERSION     DAOREF010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56220_length_1796_cov_4.295807"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1051)
                     /locus_tag="PKI33_13465"
     CDS             complement(215..1051)
                     /locus_tag="PKI33_13465"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNM38072.1"
                     /translation="MKLGIVTDSTADLPSYLIEQHEIQVVPTVLILEGKEYKDGIGIS
                     REEFYNRLPTLRTPATTAAPSIGDFLTPYQSLLNSGCDHILSIHVSGKLSGVVNIARR
                     AAEEFPGKVTCVDSGSLSMGIGFQVLAAAESADLGVKVALEALASTERRTKVFAALDT
                     MEYLKRSGRVPAVVANIGGLLSIKPVVELRNGEVKPMGINRTTSQADAFILGKLLEVG
                     EMERLAILHTNAEDRAQRLLQGMMERSHMAVPRDVLYVNVTAVIGTHLGPHCIGFAAV
                     RK"
     gene            1195..1524
                     /locus_tag="PKI33_13470"
     CDS             1195..1524
                     /locus_tag="PKI33_13470"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNM38073.1"
                     /translation="MANTESLENVVLELRRGVIVLAALSQLGSPEYGYSLLKKLADLG
                     LEVDQGTLYPLLRRLETQGLLESVWKLEEARPRRYYVISAEGKKLLPKMKKEWAGIVS
                     VMDKMLS"
     gene            1540..>1796
                     /locus_tag="PKI33_13475"
     CDS             1540..>1796
                     /locus_tag="PKI33_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38074.1"
                     /translation="MNLIDRYVSEVGKHLPLLNGREDIEKELKSTLEDMLEDRAQSTG
                     RTRDEALEIEVLKEYGSPQKIASTYNPHPYLIGPKLFPFFL"
BASE COUNT          480 a          423 c          432 g          461 t
ORIGIN      
        1 gcgcaggcgg cggcactctg atcaacctcg tagaaagcgg tgcagattgg ctcacgggcg
       61 gtcagttcgg accagaaggc ggcttggctg ttacgattgt gttggcaatc ggaattttgg
      121 tcattttgtt ttggaagaat aaagcgactg ttcaagaaat atctgcacag taaacctaaa
      181 agctcgcttg agttaagact caagcgagct ttttttattt ccttactgct gcaaacccaa
      241 tacaatgcgg acctaaatgt gtgccaataa ctgccgtcac gtttacatat aatacatctc
      301 ttggcaccgc catgtgcgaa cgttccatca ttccctgtaa taaacgctgg gcacgatctt
      361 cagcgttggt atgcaaaatc gctaaacgct ccatctcccc cacttccaac agctttccca
      421 atataaatgc atctgcctga ctggttgtgc ggttaatccc cattggttta acttccccat
      481 tcctcaactc gaccacaggc ttaatggaga gtaaaccgcc aatattggca accaccgccg
      541 ggacacgtcc actgcgtttg agatattcca ttgtatctaa cgcggcgaaa accttcgttc
      601 tgcgttctgt cgatgcgagt gcttccaacg caactttcac accaaggtcc gcggattcgg
      661 cggcggcgag cacctgaaag ccaatgccca tcgaaagcga gccactgtcc acacaggtga
      721 ctttgccggg gaattcttcg gcggcgcggc gagcaatatt taccacaccc gataatttgc
      781 ctgaaacatg gatcgacagg atatggtcac agcccgagtt gagcagagac tgatacggcg
      841 taagaaaatc tccaatggac ggcgcggcag ttgtcgccgg ggtgcgaagc gtaggcaggc
      901 ggttataaaa ctcttcgcgc gagatgccga tcccatcctt atattctttt ccttccaaaa
      961 taagaactgt aggcaccacc tggatctcat gttgttcgat tagatacgat ggcaggtctg
     1021 cggttgagtc agtgacaatt cctagtttca tagggcaaag tgtagcatat ttggatatac
     1081 cgttgttcac catgaacttg cgtcacgagc ccctaaaaat agcgtgaagg tcacctgata
     1141 ctttaatctt gacatactgt atattacaca gtataatgtc gtaaaggatg agatatggca
     1201 aacacagaat ctctcgaaaa tgttgttttg gagttaaggc gcggtgtaat cgtattggcg
     1261 gcgcttagtc aattaggctc gccagagtac ggatattcac tgctgaagaa actcgcagac
     1321 ctcggcttgg aagtggatca gggtacgttg tatcccctcc tccgccgttt ggaaacacag
     1381 ggtctgcttg agtcagtttg gaagttggaa gaagcgcgtc cacgtcgtta ttatgtgatc
     1441 agtgcagaag gcaaaaaact tctgccaaag atgaaaaaag aatgggctgg cattgtctct
     1501 gtcatggaca agatgctctc ataaagaaag ggaagaatca tgaatcttat tgatcgttat
     1561 gtttctgaag ttggcaagca tttgccgtta ttgaatgggc gtgaagacat tgaaaaagag
     1621 ttaaagtcca cgctggaaga tatgttggaa gaccgcgctc aaagtaccgg gcgcactcgt
     1681 gatgaagcac tcgaaatcga agtcttgaaa gaatacggct cgccgcaaaa aattgcatcc
     1741 acctataacc cacatccata tttgatcggt ccaaaactgt ttccgttttt tctctt
//
LOCUS       DAOREF010000720         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56229_length_1796_cov_2.933372, whole genome shotgun sequence.
ACCESSION   DAOREF010000720 DAOREF010000000
VERSION     DAOREF010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56229_length_1796_cov_2.933372"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1796)
                     /locus_tag="PKI33_13480"
     CDS             complement(<1..>1796)
                     /locus_tag="PKI33_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNM38075.1"
                     /translation="VDDGSLIEVMANEVEEQAVKFRQESIKEGTLAESFPVPYVPWSE
                     LNDNLSEFVHLELGHSTEAEIEIPLASHFGHDERFGGRLKPFIDYLAPIVDDGEQVFI
                     VSRQAQRLRELWFEHYPDSEHTNLEFTEASLSEGFTLKDQSLLSIHLITDSEVFGWER
                     PQPRTRQRQTADTPEAMYADLEAGDYVVHVDHGIGRFAGLIQRQLDGHEREYLTVEYE
                     RGDILYVPVHQADRLTRYVGADGGKPSLDNLGGQAWAETKEKVKEAVQKVAEDLLDLY
                     ARRQVVEGYAFSPDTQWQKELEDSFPYVETEDQKRAIVDIKRDMERARPMDRLLCGDV
                     GYGKTEVALRAAFKAVMSGKQVAVLVPTTVLAQQHFETFSQRLAAFPVKVEMLSRFRT
                     PREQTEILHQLSIGEVDIIIGTHRLISSDVVFKDLGLVVIDEEQRFGVTHKEHLKKLR
                     TEVDVLTLTATPIPRTLYMALTGVRDISNLNTPPEERLPIITHVGPYSPRLVRQAILR
                     ELERGGQIFFVHNRVQTIDAMKAHLEQLVPEARVDIGHGQMAEGQLSAVMHRFGNAEI
                     DILLSTTIIESGLDIPNANTLIVDRADTFGLA"
BASE COUNT          450 a          486 c          436 g          424 t
ORIGIN      
        1 cgccaagccg aaggtgtcgg cacggtctac gatgagggta ttggcattgg gaatgtctaa
       61 gccagactca ataatggtag ttgaaagcaa aatatcgatc tcagcattgc caaaacgatg
      121 catgaccgcc gaaagttggc cctctgccat ttgcccatgc ccaatatcca cacgggcttc
      181 agggacaagc tgctccaggt gtgctttcat cgcatcaata gtctgcacgc ggttgtggac
      241 aaagaaaatc tgcccaccac gctccagctc gcgcaaaatg gcttgacgca ccaaacgtgg
      301 cgagtacggt ccgacatggg tgatgatcgg gaggcgttct tctggcggag tgttgaggtt
      361 ggaaatatcg cggacgccgg tcaatgccat atagagcgta cgcggaatcg gcgtagcggt
      421 gagcgtgagc acatccactt cagtgcggag ttttttgagg tgctctttgt gagtcacacc
      481 gaagcgttgt tcttcgtcaa tgaccaccaa acccaagtct ttgaaaacaa cgtcagatga
      541 aatcagacga tgtgtgccga taataatgtc cacttcgcca atcgaaagct gatgcaaaat
      601 ctccgtctgt tcgcgaggcg tgcggaagcg tgaaagcatt tccactttga ccggaaatgc
      661 ggcgagacgc tgagagaacg tttcaaagtg ctgttgcgcc aataccgtcg tcggcacgag
      721 cactgccact tgcttgccgc tcatcacggc tttgaaagca gcccgcaaag cgacttccgt
      781 tttgccatag cccacatccc cacacaacag acggtccatt gggcgggcgc gttccatatc
      841 gcgtttgata tcaacaatag cgcgtttttg gtcttcggtt tcaacataag gaaagctgtc
      901 ttcgagctct ttttgccact gagtatcggg agaaaaagca tagccctcta ctacttgtct
      961 acgcgcatat aaatccagta agtcttcggc tactttctga accgcttcct taaccttttc
     1021 cttggtctct gcccatgcct gcccgcctaa gttatctaac gaaggcttgc caccatcggc
     1081 gccaacatag cgcgtaagtc gatccgcttg atggacggga acatagagaa tatcgccgcg
     1141 ctcatattca acggtgaggt attctcgctc atgtccgtcc aactgtcgct ggataagacc
     1201 cgcaaaacga ccaatgccat gatccacatg aacaacgtaa tcccccgctt ccagatccgc
     1261 gtacatcgct tcgggtgtat ccgctgtttg acgttggcgg gtgcgcggct gtggacgttc
     1321 ccagccaaag acttccgagt cagtaatgag atgaatagag agcagagatt ggtctttgag
     1381 cgtgaaacct tcagaaagcg aggcttcggt aaattcgaga tttgtatgtt cagaatcagg
     1441 gtaatgctcg aaccacaatt cgcgcaaccg ttgtgcctga cgcgaaacga taaagacctg
     1501 ctcaccatca tccacaatcg gagcaaggta atcaataaat ggtttgagcc gcccaccaaa
     1561 acgctcgtca tgaccgaaat gcgaagcaag cggaatttca atttcagcct ctgtggaatg
     1621 ccccaactca agatgaacga attcactcaa gttatcgttc aactcagacc atggcacata
     1681 cggcacaggg aaactctcgg cgagcgttcc ctctttgatg ctttcctgcc taaattttac
     1741 cgcctgctct tccacttcat ttgccatcac ttcgatcagg ctgccatcat ccacca
//
LOCUS       DAOREF010000721         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56335_length_1794_cov_4.385854, whole genome shotgun sequence.
ACCESSION   DAOREF010000721 DAOREF010000000
VERSION     DAOREF010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56335_length_1794_cov_4.385854"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKI33_13485"
     CDS             <1..490
                     /locus_tag="PKI33_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNM38076.1"
                     /translation="VVLIEALKSIKITPRDLYFVFTTQEEVGTRGAGTAAYSIDPEIG
                     IAIDVTGTGDTPASLKMSMELGKGPCVKVRDPGMLSDMRVVEWMASTAEKAKIPYQRE
                     VLLVGSTDAKAIQVTRAGVMSGALSIPCRYIHSASEMVDFEDLKNSVKLLTAMLSKPI
                     SF"
     gene            578..1051
                     /locus_tag="PKI33_13490"
     CDS             578..1051
                     /locus_tag="PKI33_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38077.1"
                     /translation="MGDYNRSTKEIAFGEVPVNVIKSIQTYIEKHNLGDILANPIMCI
                     VSTSEKIKKGLFGGGPGPKLLIQTAILTDRWLILADNVDQNALYIKSMQLRDITVEDY
                     EQSSFYKMIPDTGMNLNGQFTDASELGSMFLPLGKDVAGEKFKSALIESVQKAKL"
     gene            complement(1180..1482)
                     /locus_tag="PKI33_13495"
     CDS             complement(1180..1482)
                     /locus_tag="PKI33_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38078.1"
                     /translation="MDTLQQVLSTFGFLIRAFGFLVLGFGITRFMLDAYYKAVWQVQI
                     AISVGFFLLLIGLTNYSSPASMGTFSIGAGVALLMHFMVKKEEAKDEEIVKETKKK"
     gene            complement(1521..>1794)
                     /locus_tag="PKI33_13500"
     CDS             complement(1521..>1794)
                     /locus_tag="PKI33_13500"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HNM38079.1"
                     /translation="HLREKQVPGSSVFWSFMAGIIVSLFFTALIGVLASLVGFYMAEL
                     SRLKNENKAFDSTKVLLIGWGRSVALQMGIGVVMILLWGAMVWTVT"
BASE COUNT          508 a          440 c          406 g          440 t
ORIGIN      
        1 ggtggtattg atcgaagcgt tgaagtccat caaaatcaca ccgcgtgatt tgtatttcgt
       61 gttcaccacg caggaagaag tcggcactcg cggagctgga accgccgctt acagcattga
      121 cccggagatt ggcatcgcca tcgatgtgac tggcacgggc gatacgccgg cttcgttgaa
      181 aatgtctatg gagttgggca agggtccctg tgtgaaggtc cgcgacccag gcatgctctc
      241 agatatgcgc gtggtcgaat ggatggcaag taccgcagaa aaggcgaaaa tcccctatca
      301 gcgtgaagtc ttgttggttg gcagcaccga tgcgaaagcc attcaagtta cccgtgccgg
      361 tgtgatgtct ggtgcgctgt ccattccgtg ccgctacatc cactctgcct cagagatggt
      421 ggatttcgaa gatttgaaga actcagtaaa attgctcact gctatgttga gcaaaccaat
      481 ttcattctaa ttaattgtag gggcgaccct tttggtaaga gcaagtcatc ttgcttaaac
      541 caagtgggtc gcccttacgg atttaaaggt aaatactatg ggtgattaca accgttccac
      601 aaaagaaatc gcatttgggg aagttcctgt caacgttata aagagcattc aaacctacat
      661 tgaaaagcac aacttgggcg atattcttgc caacccaatc atgtgcatcg tctctacctc
      721 tgaaaaaatc aaaaagggac tgttcggcgg cggaccgggt ccgaaactgt tgattcaaac
      781 cgccatcctc accgaccgct ggctcatcct cgccgataac gtcgaccaga atgcgctgta
      841 tatcaaatcc atgcaactgc gagatattac ggtggaagat tacgaacaaa gttcattcta
      901 caaaatgatc cccgatacgg gtatgaactt aaacggacaa ttcactgatg ccagcgaatt
      961 gggttcgatg tttttaccgc tagggaaaga tgtcgccgga gaaaaattca aatccgcgtt
     1021 gatcgagtcc gtgcagaagg cgaagttata atgatgtcat tctgagcgat agcgaagaat
     1081 ctctacgata atgtaagaga cccttcgctc cgctcagggt gacatacttg taaagaaaag
     1141 gcttgaacgg tatcgttcaa gccttttctt tcatctttcc tatttcttct tcgtctcttt
     1201 tacgatttct tcgtctttcg cttcttcctt cttgaccata aaatgcatta gcaatgctac
     1261 ccctgcgcca atggaaaatg tgcccataga agcgggactg gagtaattcg tcagcccgat
     1321 aagcaagagg aagaatccaa ctgaaatggc aatttgcacc tgccaaacgg ctttgtagta
     1381 cgcgtccagc atgaagcggg taatgccaaa gcctaatact agaaacccaa acgcacgtat
     1441 taaaaagcca aacgtggaaa ggacttgttg aagggtgtcc atttgtttct ccttttagtg
     1501 aatgataatc caaaactagg ttatgtcact gtccatacca tcgcgcccca caacaaaatc
     1561 atcacaacac caatgcccat ttgcaaagca accgaacgcc cccacccgat cagcaaaact
     1621 ttggtcgagt caaaagcttt gttctcattc ttcaggcggc tcaactctgc catgtaaaaa
     1681 ccaaccaaag atgccaagac accgatcaaa gccgtgaaga ataagctgac gataattcct
     1741 gccataaacg accagaatac cgaactgccc ggcacttgtt tctcgcgcaa atga
//
LOCUS       DAOREF010000722         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56639_length_1789_cov_6.012687, whole genome shotgun sequence.
ACCESSION   DAOREF010000722 DAOREF010000000
VERSION     DAOREF010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56639_length_1789_cov_6.012687"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="groEL"
                     /locus_tag="PKI33_13505"
     CDS             complement(<1..931)
                     /gene="groEL"
                     /locus_tag="PKI33_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNM38080.1"
                     /translation="MAAKQLVFSEDARRKLKNGMNVVANAVSATLGPKGRNVAIDRKF
                     GSPTITHDGVSVAKEIELEDPFENMGAQLLKEAAQKTNDIAGDGTTTSTVLAHAIVNE
                     GLKALEAGYNPMLLKRGIELATETIVAELKKNSTKIDTKEEIASVATNSAADEEVGQL
                     IADVMDKVGKDGVITVEESKTMQFETEFVEGMQFDRGYLSPYFITNADQMEAQIGDAY
                     VLIYDKKISAAQDIVPLLEKLVQLGKRELVIIAEDVDGEALATLILNKIRGMLNVLAI
                     KAPGFGDRRKAMLQDIAVLTGGEVISEEMGRKLE"
     gene            complement(967..1269)
                     /gene="groES"
                     /locus_tag="PKI33_13510"
     CDS             complement(967..1269)
                     /gene="groES"
                     /locus_tag="PKI33_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNM38081.1"
                     /translation="MAKNNVSFKPLGSRVLIETIEQEEITASGIILPETAKEKPQQGK
                     VLAAGPGDRNDKGERVAMDVKTGDVVLFAKYSGTEVKMDGKKLLILRESDILGIVG"
     gene            complement(1518..>1789)
                     /locus_tag="PKI33_13515"
     CDS             complement(1518..>1789)
                     /locus_tag="PKI33_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38082.1"
                     /translation="LALDGSTVYYYDILASELAALSTPKNDKCPQALEVAAIIENSEY
                     ISDPNIATDMAVVRTICSFSPETTNTGSEPTPTAESATPEVTPTP"
BASE COUNT          393 a          457 c          462 g          477 t
ORIGIN      
        1 attcgagctt gcgtcccatt tcttcagaga tcacttcgcc accagtgagg acagcaatat
       61 cctgaagcat agccttgcgg cgatcaccga agccaggggc tttgatggcg agcacgttga
      121 gcatgccacg gatcttgttg aggataaggg tggcgagcgc ttcaccatcg acgtcttcag
      181 cgatgatgac caattcgcgc ttgccgagct gaacgagttt ctcgagcaaa ggaacgatgt
      241 cctgagcagc ggagatcttc ttgtcgtaga tcaacacata ggcatcgcca atctgggctt
      301 ccatctggtc ggcgttggtg atgaagtagg gggagaggta accgcggtcg aactgcatac
      361 cttcgacgaa ttcggtttcg aactgcatcg tcttggattc ttcaacggtg atgacgccgt
      421 ctttaccgac cttgtccatc acgtcggcga tgagctgacc gacttcttcg tctgccgcag
      481 agttggtggc gacagaagca atttcttctt tggtatcgat cttggtggag ttcttcttga
      541 gttcggcaac gatggtttcg gtggcgagtt caatgccgcg cttgaggagc atggggttgt
      601 aaccagcttc aagagccttc aaaccttcgt tcacgatcgc gtgagcgaga acagtggagg
      661 tagtggtacc gtcaccggca atatcattgg tcttctgggc ggcttccttg agaagctgag
      721 cacccatgtt ttcaaacgga tcttcgagtt cgatttcctt ggccacagac acgccatcgt
      781 gggtgatggt gggggaacca aacttgcgat cgatggctac attgcgtccc ttaggaccaa
      841 gagtagcgga taccgcattt gcaacgacat tcatgccgtt ctttagcttg cggcgggcgt
      901 cttcggaaaa taccagttgt ttagctgcca taattcactt ccttatatct agaaatttgt
      961 ttgggattag ccgacgatgc cgagaatatc gctctcgcgc agaatgagca atttcttgcc
     1021 atccatcttc acttcggtac cagaatactt ggcgaacaaa actacatcac cagtctttac
     1081 atccatggcg acgcgctcgc ccttgtcgtt gcggtcacca ggaccagcag ccaagacctt
     1141 gccctgctgc ggtttttcct ttgcggtttc ggggaggatg atcccgcttg cggtgatttc
     1201 ctcctgttca atggtctcga tcagcacgcg gctgccgagg ggtttgaacg aaacattgtt
     1261 ctttgccatt tgatgcctcc tgtaaggttg atacttttga ttttttagga taatcacaga
     1321 attagcactc aatacaactg agtgctaaag tctatcttac acagttaaaa agtcaacgtc
     1381 aaggggatat ataaaaattc ttacatttct ctgatgacgc ttcgcccgcc tctttctgga
     1441 cccgctccct gcctcgccta cccgagcctt atcctcgctg taccctggct ctgcaggcgc
     1501 taggaccctc tcgtcaatca aggcgtgggc gtcacctcag gggtggctga ttcagccgtc
     1561 ggcgtgggtt cagagccggt gtttgttgtc tcaggagaga acgaacagat cgttcgaacc
     1621 acagccatat ccgtagcaat gttcgggtct gaaatatatt cagagttttc gataattgcc
     1681 gcaacctcta gcgcctgagg gcatttatca tttttcgggg tactcaacgc cgccaactca
     1741 gaggcaagga tatcgtagta ataaaccgta ctgccatcta atgccaatg
//
LOCUS       DAOREF010000723         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56685_length_1789_cov_2.141869, whole genome shotgun sequence.
ACCESSION   DAOREF010000723 DAOREF010000000
VERSION     DAOREF010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56685_length_1789_cov_2.141869"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1238
                     /locus_tag="PKI33_13520"
     CDS             270..1238
                     /locus_tag="PKI33_13520"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM38083.1"
                     /translation="MKYFVTGTTGFVGGVLAKKLREAGHEVNASVRSPEKAKDLQALG
                     VKLFKGDVTDKESMREAMTGVDGVYHVAGWYKVGTKDKSGGVQVNINGTRNVLELMQE
                     LKIAKGVYTSTLAINSDTKGVLADEKYHFTGEHLSEYDRSKAEAHKLADEFIAKGLPL
                     VIVQPGLIYGPGDTSSVRASLLDLLKGRLPMLPRQTAMCWAHVDDIVHGHILAMEKGK
                     VGESYIICGEKYELYEAFKLAAQTAGVRAPMAAAPGLMKAMSVLVKPFDALMPDSYTS
                     EGLRVISGVTYIGDNSKAKRELGYDPRPVSEGWVETVKYEKQLSGR"
     gene            1361..>1789
                     /locus_tag="PKI33_13525"
     CDS             1361..>1789
                     /locus_tag="PKI33_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38084.1"
                     /translation="MALPNPESTPQTPPPLTPEQLAELREQERKQKQIMAGIIAVVVL
                     LVALLGIAIYFLLQPTTPTDKIRDVFIIVVALETLVIGVALIVLVIQLASLINLLQNE
                     VRPILQATTETVNTLRGTAEFLGENFVEPVIKLNGYLAGLK"
BASE COUNT          480 a          432 c          473 g          404 t
ORIGIN      
        1 gatacaaact ggctttttgc acattcaccg ccgccgcaat atcagacatg gacgcgccat
       61 gaaagccctt ctggcgaaag acctgtgctg ctgcatccag aacatcgtct cgggtcatat
      121 atctccttcc ctaccgaacg gtaggtaggc agagtttact actgtcatta gtgagagtca
      181 agagatttag ccctcctcta cacaacttag gggatgtaaa aattacaaga gtagaattgt
      241 ctgggttcat aacctaaagg agaacgaaca tgaaatattt tgtgacaggc acaaccggct
      301 ttgttggcgg ggttttggca aaaaaactgc gcgaagccgg gcatgaggtc aacgcctcgg
      361 ttcgctcgcc ggagaaggcg aaagacctgc aagcgctcgg cgtgaaattg ttcaaaggtg
      421 atgtgaccga taaagagtcc atgcgtgagg cgatgaccgg tgtggacggg gtttaccatg
      481 tggcaggctg gtacaaagtt ggcacaaaag acaagagcgg cggggtacag gtcaacatca
      541 acggcacacg caatgttttg gaattgatgc aggaattgaa gatcgccaaa ggtgtataca
      601 ccagcacatt ggcaatcaac tcagacacca agggcgtact cgcagacgag aagtaccact
      661 tcacaggcga acacctcagt gaatacgacc gttccaaagc agaagcgcac aaactggcgg
      721 atgaattcat cgcgaaggga ctgcctttag tcatcgtcca accggggctg atctatggac
      781 ctggcgatac ttcatcggtc cgcgcctctt tgctggacct gttgaagggt cggctgccca
      841 tgttgcctcg tcaaaccgcc atgtgctggg cgcatgtaga tgatattgtc cacggtcata
      901 ttctggcgat ggaaaaagga aaagtcggcg aaagctatat tatttgcggt gaaaagtatg
      961 agctatatga agccttcaaa cttgccgcac aaacggcggg agtccgcgca ccgatggcgg
     1021 ctgcgcccgg gcttatgaaa gccatgtcgg tattggtcaa gccttttgat gcgctgatgc
     1081 ccgattcata tacatcggaa gggttgcgag tcatatcggg tgtgacatac atcggcgaca
     1141 atagcaaagc caagcgcgag ctgggttacg atccgcgccc ggtgagcgag ggctgggtgg
     1201 aaacggttaa gtacgaaaaa caattatcag gacgctagac agaaccaagt ttggcagaag
     1261 aaaaaagcca gatggataga cacaagcgcg gcacgggaaa gaggcgactt gtttcatcct
     1321 ctggcttttt ttcgtatatc taatctgcta taatacagac atggcactgc ccaatcctga
     1381 atccactcca caaactcccc ctccgttaac tcctgaacaa ttggctgaac tgcgcgaaca
     1441 ggaacgcaag caaaaacaga tcatggcggg cattattgcc gtggtcgttt tgttggttgc
     1501 cttgcttggt attgcaattt atttcctgtt gcagcccacc acccccaccg acaagatccg
     1561 cgacgtattc attattgtcg tggcgcttga gaccctggta atcggcgtgg cgttaatcgt
     1621 gcttgtcatt caattggcaa gtctcatcaa cctgcttcag aacgaagtac gaccgatctt
     1681 gcaagccacc accgaaaccg tcaacaccct gcgcggtact gcagaatttt tgggagaaaa
     1741 ttttgttgag ccagtgatca aacttaatgg ctatttggca ggcttaaag
//
LOCUS       DAOREF010000724         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_56936_length_1784_cov_3.627530, whole genome shotgun sequence.
ACCESSION   DAOREF010000724 DAOREF010000000
VERSION     DAOREF010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56936_length_1784_cov_3.627530"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..730
                     /locus_tag="PKI33_13530"
     CDS             140..730
                     /locus_tag="PKI33_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNM38085.1"
                     /translation="MSKQPSSYLSSKLEGRLKADKSGNSIFALEPIKKGELIAVFGGV
                     VYEWETFIHLPERERMLCLQVEDRHFLVPRPIGEGDYVNHSCNPNAGLSGQIGLVAMR
                     DIQIGEEVCFDYAMCDTMPYDEFDCLCGAPNCRGRVGGNDWQRPELQKRYAGFFSPHV
                     QRRIDAQKAERRAFERAMRNAKSGALAKPVAATMYK"
     gene            complement(799..1467)
                     /locus_tag="PKI33_13535"
     CDS             complement(799..1467)
                     /locus_tag="PKI33_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38086.1"
                     /translation="MHKRLAIILFVLFSLSIFVTACGGMDQDTEIAVIVALTQTAAAQ
                     QQPQMENTQTEASATVAATEAPVIASTDTPVSVLSGYQPYALCADLNASASANNGLAG
                     TLSSPVPFNDFITGQTGTACQILYSTTNAALPADADPFRATTSMLAAQGWAEDMNYGA
                     GGATGLTTAFRKDSTLCMVGYERRPVDRALCSSDEPIGSCFEKLAPEQIVYTLTLTCS
                     TYFP"
     gene            complement(1586..>1784)
                     /locus_tag="PKI33_13540"
     CDS             complement(1586..>1784)
                     /locus_tag="PKI33_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNM38087.1"
                     /translation="HDKVIIVDNSIVVTGSLNFSSSADEENEENVVILDNPEIAALYL
                     QEYQKLWDQAMSVPTGAFTCE"
BASE COUNT          480 a          404 c          407 g          493 t
ORIGIN      
        1 gcataatctg aacggttaaa aacctttaca tgcaggttat ttgatgtatc attcatttat
       61 cgatggcgtt tttcgcggct cgtccaccgc gggaggcgct gttaatcttt aatctcagaa
      121 acgaaaggtg attttatgaa tgtccaagca accaagttcc tatctgtctt ctaagttgga
      181 aggtcgcctg aaagccgata aaagcgggaa cagcattttc gctctcgaac ccattaagaa
      241 gggcgagtta attgctgttt tcggcggcgt tgtgtatgaa tgggagacct ttatccacct
      301 acccgaacgt gagcgcatgt tatgcctcca ggttgaagat cgtcatttcc tggtgccgcg
      361 ccccattggt gagggagatt atgtcaatca ctcgtgcaac ccgaacgcag gtctttcggg
      421 ccagattgga ctcgttgcta tgcgagacat ccaaatcggc gaggaagttt gtttcgatta
      481 tgccatgtgc gataccatgc cttacgatga gttcgactgt ctgtgcggtg cccccaattg
      541 ccgcgggcgc gtaggcggaa acgactggca acgaccggaa cttcagaaac gctatgcagg
      601 tttcttttca ccgcatgttc agcgccgcat cgatgcgcaa aaagcagaac gacgcgcttt
      661 tgaacgtgcc atgcgcaatg ccaagagcgg cgcgcttgcc aagcctgtag cagcaaccat
      721 gtataagtaa tgaataagat tagacctcgg aagtaacttt ccgaggtctt tttttattta
      781 aaaatcgata tatagactct atgggaaata agttgaacaa gtaagggtta gagtataaac
      841 aatctgttca ggggctaatt tttcaaaaca actacctata ggttcatctg aactgcataa
      901 agcacggtct acgggtcttc tttcataccc taccatacat aatgtagaat ccttgcggaa
      961 tgcagtcgtt aaaccggttg ctccacctgc gccataattc atatcttctg cccagccctg
     1021 agccgcaagc atggaagtgg ttgctcggaa cgggtctgca tcagcaggta aagcagcatt
     1081 ggttgttgaa tataagatct ggcaggctgt gcctgtttga cctgtaatga aatcattaaa
     1141 aggaaccggc gaagagagcg tgcctgccaa cccgttgttt gcagaagctg aagcgttcaa
     1201 gtctgcacac aaagcatagg gttggtatcc actcaataca ctgacagggg tatctgtgga
     1261 agcaataaca ggcgcttcag tcgccgccac agtagcagaa gcttccgttt gtgtattctc
     1321 catttgaggt tgctgttgag ctgctgccgt ttgggtcaac gcaacaataa cggcaatttc
     1381 tgtatcctga tccatacccc cacaagctgt tacaaaaata gataaagaaa ataaaacgaa
     1441 gagaattata gctagtcttt tgtgcatgcg tgaagtttcc ttttcctaca taaaatatga
     1501 gacgtttctc aacgtctcat tcatgaattt agttgcttct ttatctatat ttgggtttat
     1561 ctctaatggc agagccattt gcaaattatt cgcaagtaaa agcaccagtc gggacgctca
     1621 tcgcttgatc ccataatttt tgatattcct gcaaatacaa ggcggcgatt tctggattat
     1681 ctaaaatcac aacgttctct tcattctctt catctgcact cgatgaaaaa ttgagcgagc
     1741 ccgttactac aatgctgtta tcaacaataa taaccttgtc atgc
//
LOCUS       DAOREF010000725         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_57196_length_1780_cov_2.884638, whole genome shotgun sequence.
ACCESSION   DAOREF010000725 DAOREF010000000
VERSION     DAOREF010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57196_length_1780_cov_2.884638"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKI33_13545"
     CDS             complement(<1..295)
                     /locus_tag="PKI33_13545"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM38088.1"
                     /translation="MTTNRPLRVFLCHSSADKPAVRELYQKLRAEPWISPWLDEEDIF
                     PGMDWNLEIQKAIRETDAILVCLSKSSITKEGYVQREIKTALDYADEKPDGTVY"
     gene            complement(365..1279)
                     /locus_tag="PKI33_13550"
     CDS             complement(365..1279)
                     /locus_tag="PKI33_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38089.1"
                     /translation="MTQDLQSQNMLMTGARQTMTAWSKVLESREEIPAIYQELFGKHF
                     AEGAPFPHVIWMPALNRTSGWTTEKLICDTPEVLHIFEKNGKQVSALSYAYEDVYSIE
                     DGNILLDAWLMVSGKTQGGEKRVTTLHFNMTSKRHFEGILHKLRRFPQEVDLDAFAAQ
                     KDTFNSLSNRNFKFMNFGRESLMPGETVHGFVFQPGMKNPFFTFFGITLYKPASLAHM
                     AVTTDRELILIQETGSDKELSPARYGGIWQYIPLRCVDSVMVEEIANDRVKLVIRYQP
                     DAVIEKVFDRSNLVELQRFCADLQKLIS"
     gene            complement(1497..>1780)
                     /locus_tag="PKI33_13555"
     CDS             complement(1497..>1780)
                     /locus_tag="PKI33_13555"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNM38090.1"
                     /translation="VLIPALDAEGPNYMIISLFEEGYGNESCCGMPENNAGGHVPAVI
                     LSPMAKSSFKDDMPYSHYSLLKTISNAWGLPYLGHAADEETSIMTSPWK"
BASE COUNT          490 a          455 c          368 g          467 t
ORIGIN      
        1 tataaactgt cccatcgggt ttttcatcgg cgtagtccaa agcggtcttt atctcgcgtt
       61 gaacatatcc ttctttggta atcgaacttt tggaaagaca tacaagaatc gcatctgttt
      121 ctcgaatagc cttttgaatt tccaaattcc aatccatacc cgggaaaata tcttcttcgt
      181 ccagccaagg ggaaatccac ggctcggcgc ggagtttttg atacaactcg cggacagcgg
      241 gtttgtccgc ggaagagtgg cagaggaaaa cgcggagagg gcggtttgtg gtcatatcaa
      301 aatttccccc gctattataa ctttccaaat cgttctgagg gtacaactcc cccatctcac
      361 tctatcagga aatcaacttt tgcaagtctg cgcaaaagcg ttgtaactct accaaattag
      421 aacggtcaaa aaccttttcg atcacagcat cgggctggta acgaatcaca agcttcactc
      481 gatcattcgc tatctcttcc accatcaccg aatccacgca gcgcaggggg atatattgcc
      541 agatcccgcc gtaacgcgcc gggctgagtt ccttatcact ccccgtctcc tgaatgagaa
      601 tcaactcgcg gtctgtggtt accgccatat gggcgagaga ggcgggttta tacaaggtga
      661 tgccaaagaa cgtgaagaac ggattcttca tccccggctg aaagacaaat ccatgcacgg
      721 tttcaccggg catcaggctt tcacgtccaa aattcataaa cttgaaattg cgattcgaga
      781 gcgaattaaa agtatccttt tgagccgcga atgcatccaa atccacttct tgagggaaac
      841 ggcgcaactt gtgcagaatc ccttcgaagt gacgtttgct ggtcatgttg aagtgaagcg
      901 tcgtcacccg cttctcaccg ccctgagtct taccactcac catcagccac gcatccaaca
      961 ggatgtttcc atcttcgatg ctatacacat cttcatacgc atacgaaagc gcactgacct
     1021 gtttgccatt tttttcaaag atatgcaata cctctggcgt atcgcaaata agtttctcag
     1081 tcgtccaccc agaagttcta ttcaaagcag gcatccaaat cacatgcggg aacggcgctc
     1141 cctctgcaaa gtgtttgcca aacagctcct gatagatcgc tggaatctct tctcgcgatt
     1201 caagcacctt cgaccacgcc gtcatcgtct gacgcgcgcc ggtcataagc atattttggc
     1261 tttgcaaatc ttgggtcatg gaatgcctct atatcgttga tgcaaacatt gtacccccaa
     1321 gaaaaaagac aaaagagaca agcatattca aaataaacat ggacgggaaa atttcccgtc
     1381 catgaaaacc atcaattcgt gatgacttat ccgtctgcta ccagtggacg aaccgtctac
     1441 atacgtagct caaagataat tttctattga cagtcattaa gtcatctaaa ttttttttat
     1501 ttccaaggtg aagtcatgat agatgtctct tcatctgcag catgcccaag atagggtagt
     1561 ccccaagcat tggatatcgt tttcagaagg gaataatgtg aataaggcat atcgtccttg
     1621 aaactacttt tagccattgg agaaagaatc acggctggaa catgcccacc cgcattgttt
     1681 tcaggcattc cacaacatga ttcatttccg tacccttcct caaataagct aataatcata
     1741 tagttaggtc cctctgcgtc caaggcgggg atgagaacat
//
LOCUS       DAOREF010000726         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_57243_length_1779_cov_5.070766, whole genome shotgun sequence.
ACCESSION   DAOREF010000726 DAOREF010000000
VERSION     DAOREF010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57243_length_1779_cov_5.070766"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKI33_13560"
     CDS             <1..449
                     /locus_tag="PKI33_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38091.1"
                     /translation="QLGECKGYPWIEELPNETTALREFSVNSGWSKFDSQFILIDDEV
                     RLYDYRGTNLVNENNLLSFLLPKKIMISELPPLEISGQFRYGFYMFFVWGDNATQEEI
                     ETYTNMINNLPGKKDFFDWGVKIDDVIFAVNEDGAFDMVECKSLQP"
     gene            complement(540..1676)
                     /locus_tag="PKI33_13565"
     CDS             complement(540..1676)
                     /locus_tag="PKI33_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM38092.1"
                     /translation="MKIQRLLLDELQKSLLRGKVLVLYGPRQAGKTTLAKDLMANTSL
                     RSKFVNADELLYREALSSQSLKTLNEVLGEAELLVIDEAQRVSEIGLNLKILIDNNPN
                     AIILATGSASFDLANKINEPLTGRKLTFTLYPLSYSELSKTLGSLETRNQLEQWLVWG
                     GYPVIATTSDLSLRERLLGELVGSYLYRDVLELGEIRRSEKIVDLLRLLAYQIGQEVS
                     IAELATSLGLSRDTVERYLDLLEKVFVIFRVGGFSRNLRKEVTKNSRYYFFDNGVRNS
                     LIQNLNPISVRNDAGQLWENFLMVERRKSNQFAERSVNAYFWRTYDQKEIDYIEEHAG
                     QLNAYEFKWGDGEIKSTTRKLFEEAYPDAKMQVINRENFEGFVM"
BASE COUNT          500 a          410 c          350 g          519 t
ORIGIN      
        1 atcaactggg tgaatgcaaa ggttaccctt ggatagaaga gttgccaaat gaaacgacag
       61 cattgcgtga gttttcagtc aatagcggct ggagcaagtt tgattctcaa tttatcttga
      121 tagatgatga agttagatta tatgactata gaggaacaaa cttagtaaat gagaataatt
      181 tattgtcatt ccttttgcct aaaaaaatca tgatctcaga attgccacct ttagaaatta
      241 gcggtcaatt cagatatggt ttttatatgt tctttgtgtg gggagataat gctactcagg
      301 aagaaattga aacctatact aatatgataa ataaccttcc cggaaaaaaa gattttttcg
      361 attggggtgt aaaaattgat gatgtcatat ttgctgtaaa tgaagatggt gcttttgata
      421 tggtcgaatg caaaagtttg cagccataat attccgtaga tttataaaaa acacctgcaa
      481 cttttcccct ccttgcctgt attacactct agtcatgaat gcgcccatcg acagcgaccc
      541 tacatcacaa aaccttcaaa gttttcgcgg ttgataacct gcatcttcgc atcgggatat
      601 gcttcttcaa atagtttccg cgtagttgac ttgatctctc catcgcccca tttgaattca
      661 taggcattca actgtcccgc atgttcctcg atgtaatcaa tttccttctg gtcatacgtc
      721 cgccagaaat aagcattgac cgaacgctct gcaaactggt tcgatttacg gcgttccacc
      781 atcagaaaat tctcccacaa ttgccctgcg tcgtttcgta ccgaaatggg attcaagttt
      841 tggatcaggc tgttgcgtac accgttatcg aaaaaatagt aacgtgagtt cttggtcacc
      901 tctttgcgaa ggttgcgaga gaatccaccc acgcgaaaaa tgacaaatac tttttcgagc
      961 aggtctaaat aacgttccac agtgtcacga cttaaaccca gacttgtggc aagttctgca
     1021 attgaaacct cctgcccgat ctggtaggca agcaagcgca gcaaatccac gatcttttct
     1081 gagcgtcgaa tctcacccag ttccaacaca tcgcgatata aataggaacc aacaagttct
     1141 cccaacaaac gctcgcgcag agacaggtcg ctggtggtag cgataaccgg ataacctccc
     1201 caaaccaacc attgttccaa ctggtttcgg gtttccaatg agccaagggt cttgcttaac
     1261 tccgaataac tgagtggata aagtgtgaag gtcagcttac gtcctgttag cggttcattg
     1321 atcttattgg cgaggtcgaa agatgccgaa ccagtcgcaa ggatgatagc attgggattg
     1381 ttatcaatta gtatctttag gttcaacccg atttcagata cacgctgcgc ttcatcaatt
     1441 accaacaatt ctgcttcacc caatacttca tttagtgttt ttaaactttg gctggacagc
     1501 gcttctcgat aaagtaactc atcggcgttg acaaattttg acctcaggga tgtgtttgcc
     1561 attaaatcct tggcaagagt agttttccct gcctgtcgtg gaccatacaa aaccaaaact
     1621 ttgccccgaa gcaggctttt ctgtaattcg tcgagtagta accgctgaat tttcataaat
     1681 tgattatact acaccctctg aatttcggat ttccaaccgc tctccctcct gcaacttttc
     1741 cccctccccg cctgtattac actctagcca tgaatgcgc
//
LOCUS       DAOREF010000727         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_57247_length_1779_cov_4.169374, whole genome shotgun sequence.
ACCESSION   DAOREF010000727 DAOREF010000000
VERSION     DAOREF010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57247_length_1779_cov_4.169374"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKI33_13570"
     CDS             <1..668
                     /locus_tag="PKI33_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNM38093.1"
                     /translation="ERTALEGGTSLAVHESQSRMWENLVGRSMPFWEHFYPDLKKTFP
                     SQLDGVSVKNFYKAINKVEPSFIRVNADEATYNLHIMLRLEIEIGMVDGSIAVKDLPE
                     IWNEKMRDYLGITPPNDAQGVLQDIHWSYGSIGYFSTYALGNIISAQLWERINKDIKN
                     LEDQIRKGQFAELREWLREKIHVYGHKYDPQDIVQKVTGSKIDSAAYVRYLTKKYSEV
                     YGL"
     gene            723..1649
                     /locus_tag="PKI33_13575"
     CDS             723..1649
                     /locus_tag="PKI33_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38094.1"
                     /translation="MRLRLFSIASLLGLLSACSFQSTPAPTPLPPDYLPTVVALTGQA
                     AFATAAALTPSATLPPTETLQPPVEQAIPPTATPTLAAGFTEYAQIRFISPGPMSSLT
                     SPFILQANIAAGESDKIQVDLLGEDGRVLYREIKKLTRNPLGVFQRFEISYEIRAVSE
                     AGYIRISTEDDYGRMQAVNTMPVLLYSVGTAQINPPGNFIYERIAMDGFKEKSNFYAG
                     QVALNGRIWPFSDQAFVVEMISTEGKVLSSRILGINGIDTQPFETVLPYKVTEPTPVR
                     LTFRQDNPLIGISDPVLGKLAYLYTIEVILNP"
     gene            1700..1773
                     /locus_tag="PKI33_13580"
     tRNA            1700..1773
                     /locus_tag="PKI33_13580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Pro,seq:cgg)
BASE COUNT          438 a          460 c          401 g          480 t
ORIGIN      
        1 atgagcgcac tgctttggaa ggcggcacat cgcttgctgt gcatgaatca caatctcgca
       61 tgtgggagaa tcttgttggt cgttccatgc ctttctggga acatttttat cccgacttga
      121 agaagacttt cccgtctcag ttggatggcg ttagtgtaaa gaacttttat aaagccatta
      181 acaaggttga accttccttt atccgcgtca atgccgatga agccacctac aacctgcaca
      241 tcatgttgcg ccttgaaatt gagattggca tggtcgatgg cagcattgcc gtgaaagact
      301 tgcctgaaat ttggaatgaa aagatgcgcg attacctcgg catcactccg ccaaatgatg
      361 cccaaggcgt gttgcaagat attcactggt cgtatggctc gattggctac ttctcgacct
      421 atgcacttgg caatatcatc tctgctcagc tgtgggaacg catcaataaa gacatcaaga
      481 atctggaaga ccaaattcgc aaaggtcaat ttgctgaatt gcgcgagtgg ctacgtgaga
      541 agattcatgt ctatggtcac aaatatgacc cgcaagacat tgttcagaaa gtcactggct
      601 ccaagatcga ctcggcggca tacgtccgtt atctcacaaa gaaatatagt gaagtgtacg
      661 ggctgtaaat tgatttgact caagagacgc cccgcctgtg ggcgtctctt ctattatcca
      721 ctatgcgact tcgactattt tccatcgcct ctcttctcgg acttttgtct gcttgttcct
      781 ttcagtccac gcctgcgccc acgcctttac cgcccgatta tttacccacc gtggttgcac
      841 tcaccggaca ggctgccttt gctacagcgg ctgctcttac accttcggca acacttccgc
      901 ctactgaaac gcttcaacca cctgtagaac aggcaatccc tccaacagct acgccaaccc
      961 tcgcagcagg ctttactgaa tatgcccaaa tccgctttat ttcgccggga cccatgtcca
     1021 gcttaacttc gccttttatt ttgcaagcca atatcgcagc gggtgagagc gataagatcc
     1081 aagtggattt gcttggcgaa gatgggcgag tactctaccg cgagatcaag aagttgacgc
     1141 gcaatccttt gggcgttttc caacgctttg agatctccta cgagattcga gccgtgtctg
     1201 aagcgggcta catccgcatt agtaccgagg atgattacgg cagaatgcaa gccgtcaata
     1261 ctatgccagt tttgctctac tctgttggta cggcacaaat aaacccacca gggaatttta
     1321 tttatgaacg tatcgccatg gatgggttca aagaaaaatc gaacttctat gccggtcaag
     1381 tagcattgaa tggacgcatc tggcccttta gtgatcaagc ttttgttgtt gaaatgattt
     1441 ccacagaagg gaaggtgtta tcctcccgta ttcttggcat taacggtatt gatacccaac
     1501 catttgagac tgtcttacct tataaagtga ccgagcctac accagtccgc ttaacctttc
     1561 ggcaggacaa tccgcttatc ggtattagtg accccgtcct tggaaaactt gcctatcttt
     1621 atacaattga agtcatcctc aacccctaat ctgacttgct aaaataaatc agaatggtat
     1681 aatctgcccg ctgttctttc ggggtgtggc tcagaccggt agagcgcacg gttcgggtcc
     1741 gtgaggtccg gggttcaaat ccccgcaccc cgacagcat
//
LOCUS       DAOREF010000728         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_57836_length_1770_cov_4.765598, whole genome shotgun sequence.
ACCESSION   DAOREF010000728 DAOREF010000000
VERSION     DAOREF010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57836_length_1770_cov_4.765598"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..>1770)
                     /locus_tag="PKI33_13585"
     CDS             complement(166..>1770)
                     /locus_tag="PKI33_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNM38095.1"
                     /translation="VENLAVLDLAFAKAKYSEELRASEPVMHERGKKNEASASLPILR
                     LIQARHPLLDQDTVVPVDVDPREGTRAIVITGPNTGGKTVSLKTVGLLVLMAQSGLHI
                     PAQSGSELPFFKSVYADIGDEQSIEQSLSTFSGHITNIIRILKQIDNRSLVIFDELGS
                     GTDPQEGAAIARAILSHLLEVGVMTLVATHYPELKTFAHSTEGVVNASLEFDIKTLRP
                     TYKLTIGLPGRSNALAIAQKLGLPQPIIDSAKAEINPLDLRADKLLDDIRKERNRTSR
                     EREKLEKQRSKLEAQNADLAKRLEKIEDERRETLAKARAEGELEVALLKRNIDSLKSQ
                     LKKASQPLTAIRAIGERMEKLEEKVEAPVERKSEQSAVTSNQGLKLGEKVTVSSLNAE
                     GVVTALGESDAEVQIGTIRVRAKMSDLIRKSQEKSEASNEKKVVSKSVSSSRSSLPAT
                     SSPGMEVDLRGLMAEDALDKMERYLEQAYLSGLPWVRIIHGKGTGKLRQAVRETLKGH
                     TYVKSFEEGASNEGGEGVTVAKLNAA"
BASE COUNT          395 a          470 c          425 g          480 t
ORIGIN      
        1 gcactggcac aggcggcggt tacaaggtcg gacatttttt tgatggcatc gtcaatggac
       61 ataaacgtct cctccataaa aattataaac gcaatcataa cccggtcaga ttacgattgc
      121 gttaagcgta atacctaaaa gtactaagca caatacatta aacccctacg ctgcgttcag
      181 ctttgccacc gttacccctt cgccgccttc attagaagcc ccttcttcaa aagacttcac
      241 gtaggtatgt ccttttaggg tctcgcgtac agcttggcgc agtttcccgg tccctttccc
      301 gtgaatgatg cgcacccaag gcaaacccga aagatatgcc tgctctaaat agcgttccat
      361 cttgtcgagc gcatcttccg ccatcaaccc gcgcaagtcc acttccatgc cgggagaaga
      421 agtagcaggt aacgaggagc gagatgacga gacactcttg cttacaactt tcttctcatt
      481 acttgcttct gatttttcct gtgacttccg aatcaaatcc gacatcttcg cccgcacacg
      541 aatcgtcccg atttgaactt ccgcatccga ctcacccaaa gctgttacaa caccctcggc
      601 attcaagctg ctgacggtga ctttctcgcc cagcttcaac ccttggttac tggttactgc
      661 agactgttca ctttttctct caaccggtgc ttctaccttc tcttcaagct tctccattct
      721 ctctccaata gcgcggatgg cggttagggg ctggctggct ttcttcaatt gagacttcaa
      781 cgagtcgata ttgcgtttga gcaacgccac ttccaactcc ccttcggcgc gggctttggc
      841 gagagtttcg cggcgttcgt cttcgatctt ttcaaggcgt tttgccaggt ctgcattttg
      901 cgcttcgagc ttggagcgtt gcttttccag tttctcacgt tcgcgcgatg tacggttgcg
      961 ttctttgcga atgtcgtcca gcaatttatc ggctcgcaaa tccagcgggt tgatctctgc
     1021 cttggcagag tcaatgatgg gttgggggag ccctaacttc tgagcgatgg ctaaggcgtt
     1081 agacctgccc ggcagaccga tggtcaactt ataggtcggg cgaagcgttt tgatgtcaaa
     1141 ttccaaagaa gcattgacca cgccttcggt cgaatgagcg aaggttttta attcagggta
     1201 atgtgtcgcg accaaggtca tcacgcccac ttcgagtaaa tggctcaaga tcgcgcgcgc
     1261 gatggcagcc ccttcctgtg ggtcggtgcc ggaaccgagc tcatcaaaga taaccagtga
     1321 acgattatca atctgtttga gaatgcggat aatgttggtg atatgtccac tgaaggttga
     1381 aagcgattgc tcaatcgatt gttcatcgcc aatatcggca tagaccgatt tgaagaatgg
     1441 caactctgag ccgctttggg cagggatgtg aagtccgctt tgcgccatga gcacaagcaa
     1501 accaactgtc tttaatgaaa ctgtcttacc acctgtattc ggacccgtga tgacgatggc
     1561 gcgtgtccct tcgcgcgggt caacatctac agggacgacc gtatcttggt cgaggagtgg
     1621 gtggcgggct tgaatgaggc gtaagatggg tagtgaagca gaggcttcat tctttttacc
     1681 tctttcatgc attacaggtt cactcgctcg taactcttca gaatactttg cttttgcaaa
     1741 tgccaggtcc aacacggcga gattttccac
//
LOCUS       DAOREF010000729         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_57957_length_1768_cov_5.107998, whole genome shotgun sequence.
ACCESSION   DAOREF010000729 DAOREF010000000
VERSION     DAOREF010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57957_length_1768_cov_5.107998"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKI33_13590"
     CDS             <1..824
                     /locus_tag="PKI33_13590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM38096.1"
                     /translation="RWLEEWNPDALIMEANPRYLASPSAVSWMHKRGRRVLGWGLGAP
                     KQSGLWKRFIHQFDGMIAYSQRGAEEYAALGFPREKIFVAHNSVSAKPEKPDDRPLTI
                     DHVTILFVGRLQARKRIDSLLRACAEIESKPRLLIIGDGPERESLQTLAKEIYPSAEF
                     IGAKHGAELKPYFQQADLFVLPGTGGLAVQEAMSYGLPVIVAKGDGTQDDLVREKNGW
                     QIEPENYEALVSTMKNALSDIARLREMGKESFRIVSEEINIEKMADTFVNALNSL"
     gene            complement(862..1296)
                     /locus_tag="PKI33_13595"
     CDS             complement(862..1296)
                     /locus_tag="PKI33_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38097.1"
                     /translation="MHKYDEFVHKELAPHLSAGEGIELTGFLYTKSLRAVVLTGGLSL
                     VGDGYYFAALTRRRMFIIATTMGIVSLNMENKGMSEILYTDIKSIKPSGFLNQKMLAI
                     ELNDGKTLVFRLNTLATNFASGQKQFIETLIEFVGSGSPRSN"
     gene            complement(1296..>1768)
                     /locus_tag="PKI33_13600"
     CDS             complement(1296..>1768)
                     /locus_tag="PKI33_13600"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNM38098.1"
                     /translation="RPNWMVVGILFGVSLFLGYAIFTALTTALFPAVNNVSGKFLCDG
                     EYKIVTTQSSPRPGQTYWSHTILCDGKDITLPAVAITGLMASAVFFVLLLILTRNWWF
                     REKAALAPFANAGVPAKGKKSAMERMSELKEMRDKNLISQVEYERKKDDIMKEL"
BASE COUNT          462 a          418 c          449 g          439 t
ORIGIN      
        1 tccgctggct cgaagagtgg aatcccgacg cgctcatcat ggaagccaat ccgcggtatc
       61 tcgcctctcc ctccgccgtg agttggatgc acaaacgcgg acgacgagtc ctcggctggg
      121 gcttgggtgc tcccaagcaa agcggactct ggaaacgttt catccatcaa ttcgacggga
      181 tgatcgccta cagtcaacgc ggcgcggagg agtatgcggc gcttggtttc ccgcgtgaga
      241 agatctttgt ggcgcataat tcggtgtcgg caaagccaga aaaacctgac gatagaccat
      301 tgacgattga ccatgtgact attttgtttg ttgggcggtt gcaagcgcgc aagcgtattg
      361 attcattatt acgcgcttgt gcagaaatag aatccaagcc tcgcttgctt atcatcggtg
      421 atggacccga acgtgaatca ttgcaaacgt tggcaaaaga aatttatcca tccgctgaat
      481 tcattggtgc gaagcatggt gcagagttga agccgtattt tcagcaagcg gatctgttcg
      541 tcctacccgg cacaggcggg cttgccgtac aggaagccat gagttacggt ctgcccgtca
      601 ttgtcgccaa aggcgatggg acgcaagatg atctagtccg cgagaagaac ggctggcaga
      661 tcgagccgga gaattacgag gcattggtct ctactatgaa aaacgcgcta tcagatatag
      721 cgcgtctgcg agagatggga aaagagtcgt ttcggatcgt ttctgaagag atcaatatcg
      781 agaaaatggc ggatacgttc gtaaatgcat taaactcgtt gtaatcgtag gggcgcggaa
      841 accgcgccct tttatttcct attaattgga gcggggagac cccgacccta caaattcaat
      901 caacgtctca ataaactgtt tttgccctga ggcaaaattc gttgccagtg tattcaagcg
      961 gaagacaagg gtcttaccat cgttgagttc aatagcaagc attttctggt tgaggaatcc
     1021 gctcggcttg atggacttaa tatcagtgta gaggatttcg ctcatgcctt tattttccat
     1081 attgagcgaa acgatgccca ttgttgtagc gatgatgaac atgcgtctgc gagtgagggc
     1141 ggcgaagtag tatccatcac cgaccaagct caaaccgccg gtaaggacaa cggctctcaa
     1201 agatttggtg tagagaaagc cggtgagttc gatcccttct ccggcggaga gatgaggcgc
     1261 tagttcctta tgaacaaatt catcatattt atgcatcaca gttccttcat gatgtcatct
     1321 tttttccgtt catactccac ctgagagatc aagttcttat cacgcatttc cttgagttct
     1381 gacatccgtt ccatcgcaga cttcttccct tttgctggca cgccagcgtt tgcaaatgga
     1441 gctagagcag ccttttcacg aaaccaccaa ttgcgtgtga ggatgagcaa caatacaaaa
     1501 aagacagcag atgccatcaa acccgttatc gcgacagcgg gaagcgtgat gtcttttcca
     1561 tcacagagga tggtatgaga ccaataagtt tgcccggggc gcggagaaga ttgcgtggtg
     1621 acgatcttat attcgccatc acacaaaaat ttacctgaaa cattgtttac agccgggaag
     1681 agcgcagttg tcaacgctgt gaaaatggca tatcctaaaa acaaacttac gccaaacaaa
     1741 attccaacaa ccatccagtt tggtcttt
//
LOCUS       DAOREF010000730         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_58014_length_1767_cov_4.354556, whole genome shotgun sequence.
ACCESSION   DAOREF010000730 DAOREF010000000
VERSION     DAOREF010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58014_length_1767_cov_4.354556"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PKI33_13605"
     CDS             <1..879
                     /locus_tag="PKI33_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM38099.1"
                     /translation="KNVAPRFDWKKWLGVTPFFLFALLFLILPSLKLFVGAFTDDAGR
                     FTLANMLALFTQQSILDSYKTSIQISAVTAIGGGFFGFLLAYSITVGGLPRSLRSMLI
                     TFSGVASNFAGVPLAFAFVATLGRTGIITAFLDEFVFQKFFGVKLYQTGFNLYSFLGL
                     SLTYMYFQFPLMVLIITPALDGLKREWREAAENLGATQFQYWSKVAMPILLPPLLGAT
                     ILLFGNAFGAYATAYALTGGTLPIVTIQIGAQIRGDVLHNPGLGYAMAMGMVIIMAIS
                     ITGYTWLQKISERWLK"
     gene            876..1706
                     /locus_tag="PKI33_13610"
     CDS             876..1706
                     /locus_tag="PKI33_13610"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38100.1"
                     /translation="MKKRSSRNFPFWSWVWFILGALYFILPLYATLDFSLQMERDVIG
                     FKAYEKAFEDKDFIETFTYSNILAFATIAVSIILIVPTAYWIRLRVPEARRAVEFITL
                     LPFVVPGIILVFGLIRIYSSPLTIPFTDIVLMQPLTTYKFGTNVLIVAGYMVLAMPYM
                     YRSVDTGMRTVDVATLTEAAQSLGASWFTIITRIILPNIRSALLSGALLTFAIVVGEV
                     TLASFLGVDAFGPYLFLLGQHRAYEPAALSFVSFLLTWAAMGLIQLFTGGQGQTAGAH
                     "
BASE COUNT          370 a          408 c          426 g          563 t
ORIGIN      
        1 aagaacgttg ctccaaggtt cgattggaaa aaatggttgg gggttacacc gtttttcctt
       61 tttgcacttt tattcttaat tctgccctct cttaaattat ttgttggggc atttaccgat
      121 gatgctgggc gttttacgct ggcgaatatg ctagcattgt tcacccagca atccattctt
      181 gattcctata aaacgagcat tcagatcagc gcagtcaccg cgattggggg cggctttttc
      241 gggttcctgc tggcatactc catcactgtg ggtgggttgc cgcgatctct gcgttctatg
      301 ttgattacat tttcaggcgt tgcatccaac tttgcaggcg tgcctttggc attcgccttt
      361 gttgctacgc ttggacgcac gggtatcatc actgcttttc tggatgaatt tgtatttcaa
      421 aaattttttg gcgtaaaact ctaccaaacc ggtttcaatc tatatagctt cctcggtttg
      481 agtctcactt acatgtattt tcaatttcca ttgatggttt taatcattac cccggcattg
      541 gatggtttga aacgtgaatg gcgtgaagct gccgaaaatt tgggcgcgac ccaatttcaa
      601 tattggtcaa aggttgccat gccgatatta cttccgccat tgttgggtgc aactattttg
      661 ttgttcggaa atgcgtttgg tgcgtatgcc acagcctatg cattgacggg tggcaccctg
      721 ccgattgtga cgatccagat cggggcgcag atccgcggcg acgttcttca taatcccggt
      781 ttggggtacg ccatggcaat gggtatggtt atcatcatgg cgatttccat cacggggtat
      841 acctggctac agaaaatatc tgaaaggtgg ttgaaatgaa aaaacgatca tcacgaaact
      901 ttcctttttg gagctgggtc tggtttattt taggcgcact gtattttatt ttgcccctat
      961 atgccactct tgatttttcc cttcaaatgg agcgcgatgt cattggtttc aaagcgtatg
     1021 agaaagcctt tgaagataaa gacttcattg agactttcac atattccaac attttggcgt
     1081 ttgcaaccat tgctgtcagt attattctga tcgtcccaac cgcatactgg attcgcttgc
     1141 gtgttccaga agcgcgccgt gctgtggaat tcatcacctt gttgccgttt gtggtgcccg
     1201 gaattatctt ggtgtttggg ttgatccgaa tttacagttc acctttgacg attccattta
     1261 ccgatattgt tttgatgcaa cctctcacga cctataagtt cggcaccaac gtgttgatcg
     1321 tggctggcta tatggtgctt gccatgccat atatgtaccg ttcggtggat acgggcatgc
     1381 gcactgtgga tgtagcaact ttgacagaag cggctcaaag cctcggcgcg agctggttca
     1441 ccatcatcac acggatcatt ctcccgaaca ttcgctcggc gctccttagt ggtgccttgt
     1501 taaccttcgc cattgtagtg ggcgaagtga ccctcgcttc cttcctcggc gtggatgcgt
     1561 tcggtccgta tctcttcctg ttggggcagc atcgtgcata tgaacctgcc gccctttcat
     1621 ttgtaagctt cctgttgaca tgggcggcaa tggggttgat ccaattattc actggcggac
     1681 aaggtcagac cgccggggca cactaatctt ttgaggagaa tggcaatggc atttctttcc
     1741 atttcaaatg tttcaaagca atacggc
//
LOCUS       DAOREF010000731         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_58139_length_1765_cov_3.312865, whole genome shotgun sequence.
ACCESSION   DAOREF010000731 DAOREF010000000
VERSION     DAOREF010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58139_length_1765_cov_3.312865"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKI33_13615"
     CDS             <1..526
                     /locus_tag="PKI33_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNM38101.1"
                     /translation="EYWLSMELAGRFASANHYIIHKRVAGSVGLKEVAVVENHHNFAW
                     REKLPDGREVIVHRKGATPAGAGVLGVIPGSMGDAGYVVRGRGVSESLESASHGAGRK
                     MSRRVAINSISKRERDDYLKERGVTLLGGGIDESPQAYKQIEDVITAQHDLVDVIGKF
                     TPRIVRMADEPGDI"
     gene            586..1227
                     /locus_tag="PKI33_13620"
     CDS             586..1227
                     /locus_tag="PKI33_13620"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNM38102.1"
                     /translation="MHIQPKDPALQEILLNYSQESDLVKRKQIEDEIWQRYGSANTAF
                     VLDMSGFSLLTRKYGIVHYLSMIRRMQLTVEPIIMGHGGRIIKFEADNCFAVFPEPAA
                     AARTAITIQHALAASNLLTSEDMDVHVAIGIDYGKILILNDDDMYGDAVNRACKMGED
                     IGKADEILISKEAMDMIPEDAQIEGKLIEVSIGGLTMPAYQIVYRTNTEEEQQ"
     gene            complement(1258..>1765)
                     /locus_tag="PKI33_13625"
     CDS             complement(1258..>1765)
                     /locus_tag="PKI33_13625"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38103.1"
                     /translation="QRLDHFNATHIRLLTTEDLAARLKPYFTREGLAVKDDVLLKMIP
                     LIRERLTTLDDCISFGAFFFKDEVTPTPEDLIAKGLDAKQSVEIAQKAYELLSAQPDI
                     SHERSEPPFREYVEASGFNAGQVFGVLRVAVTGQKVSPPLFESMEIIGRDICLKRIQN
                     AISILEKM"
BASE COUNT          459 a          425 c          464 g          417 t
ORIGIN      
        1 ggaatactgg ctttcaatgg aactggctgg tcgcttcgct tcggcgaatc actacatcat
       61 ccacaagcgc gttgcagggt cggttgggct gaaagaagtg gcagtggttg aaaatcacca
      121 caactttgct tggcgtgaaa aactgccgga tggacgcgaa gtcatcgtcc accgcaaggg
      181 agccacgccc gcgggtgctg gcgttctcgg cgtgatcccc ggctcgatgg gagatgcagg
      241 ctacgtcgtc cgcggaagag gcgtgagcga gtcgcttgag tccgcttcac atggagccgg
      301 tcgcaagatg agccgcagag tcgccatcaa ctccatcagc aagcgcgaac gggacgatta
      361 cctcaaagag cgcggcgtca ccctgctcgg cggaggcatt gacgagtcgc cgcaggcgta
      421 caagcagatc gaagacgtca tcaccgccca gcacgatcta gtggatgtga tcggcaagtt
      481 caccccgcgc atcgtccgca tggcggatga gccgggcgat atttaggtaa aaagggtcat
      541 gggaatgcaa gccatttcca tgaccctttt cgatataata aacttatgca tatccaaccc
      601 aaagaccctg ccttgcaaga aatcttacta aactattcac aagaatcgga ccttgtgaaa
      661 cgtaagcaga tcgaagatga gatctggcag cgatacggca gcgcgaacac tgcttttgtt
      721 ttagatatgt ctggtttctc actgctcact cgcaaatatg gcatcgttca ttatttatcc
      781 atgattcgcc gtatgcaact aacagttgaa cccatcatta tggggcatgg tgggcgcatt
      841 ataaaatttg aagcggataa ctgtttcgca gtttttccag aacctgcagc ggcagcacga
      901 actgccataa ccattcaaca tgcgttagcg gcatctaacc tgcttaccag tgaagatatg
      961 gatgtgcatg ttgccatcgg cattgattat ggaaaaatcc ttattctcaa tgatgatgac
     1021 atgtacggcg atgctgtcaa tcgcgcttgc aaaatgggag aagacattgg caaagctgat
     1081 gaaatcctca tttcaaaaga agccatggat atgattccag aagatgctca aattgagggt
     1141 aaattgatcg aggtcagcat cggcgggctg accatgccgg cgtatcaaat tgtttatcga
     1201 accaatacag aagaagaaca gcaataaaaa aagacggagc aaatgctccg tcttttttta
     1261 catcttttcc aatattgaaa tcgcgttttg aatgcgtttc aggcagatat cgcgcccgat
     1321 gatctccata ctctcaaaca acggcggact gaccttctga ccagtcactg cgacgcgtaa
     1381 cacaccaaag acttgccctg cattgaatcc gctggcttca acatattcgc ggaagggcgg
     1441 ttcgcttctt tcgtgactga tatctggttg cgcagatagg agttcatatg ccttttgagc
     1501 aatctctacg gattgttttg catccagccc tttggcgatg aggtcttcag gagtcggtgt
     1561 gacttcgtct ttgaagaaaa atgcgccaaa ggaaatgcaa tcatccagag ttgtaaggcg
     1621 ctcgcggatg agaggaatca tcttcaacag aacgtcgtcc ttcacagcga gtccctcacg
     1681 ggtgaagtaa ggcttgagac gagctgccaa gtcttcggtc gtcaggaggc ggatgtgagt
     1741 cgcgttgaag tggtcgaggc gctgg
//
LOCUS       DAOREF010000732         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_59198_length_1749_cov_1.978749, whole genome shotgun sequence.
ACCESSION   DAOREF010000732 DAOREF010000000
VERSION     DAOREF010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59198_length_1749_cov_1.978749"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1463)
                     /locus_tag="PKI33_13630"
     CDS             complement(90..1463)
                     /locus_tag="PKI33_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNM38104.1"
                     /translation="MPQPKTSISKSGTFVSQRPPVEKRNFVSSAVEETIATVSQAIAD
                     PELAWLFENCFPNTLDTTVKHSTINGKPDTFVITGDIEAMWLRDSTAQVWQYVPLINT
                     DAKLKDLIAGVIHRQTQCILIDPYANAFNDGPTGSDWETDLTSMRKELHERKYELDSL
                     CYPVRLAHHYWKTCGDASIFDADWQVATHLIVKTMREQQRWDGPGPYRFQRVTSVSHD
                     TVPLGGFGNPTRPNGLIHSAFRPSDDACIYPFLIPSNFFAVRTLEQLAEIYSTQLKDS
                     AFADECTSLAKEVHSALGNFAIAQHPTHGAIYAYEVDGFGNALMMDDANIPSLLSLPY
                     LGAVSVDDPLYQSTRRFVLSLDNPYFFKGRAAEGIGGPHVGLGMIWHLSIIMRALTSK
                     DENEIIHCLSMLKHTHAGTGFMHESFDKEEPSQFTRKWFAWANSLFGELILKTYNERP
                     RILKILL"
BASE COUNT          425 a          381 c          464 g          479 t
ORIGIN      
        1 cttctttcgt cattgtttct agatcgttat ccatggtatc tcctgaaacg gcttctataa
       61 aaaaggaata tgtagtgtct tttgcgttgt tataagagta tcttcaaaat gcgcggacgt
      121 tcgttatatg tctttagaat caattcaccg aataatgagt tcgcccaagc aaaccacttg
      181 cgagtgaatt gacttggctc ttctttatcg aaagactcgt gcatgaaacc tgttccggca
      241 tgtgtatgtt tgagcatgga tagacaatga atgatctcat tttcatcctt gcttgttaga
      301 gcgcgcatga tgatactcag atgccagatc atgccaagac cgacatgtgg tccgccaatg
      361 ccttcggcgg cacgaccttt gaagaaatag ggattatcca agctcaaaac gaatcgacgc
      421 gtggattgat agagcgggtc atctactgaa acggctccga gataggggag tgataacagg
      481 cttgggatgt tggcatcatc catcatcaag gcgttaccga acccatctac ttcgtatgca
      541 tagatagcgc cgtgcgtggg gtgctgagca atcgcaaagt tgcccaatgc cgaatggact
      601 tcctttgcaa gggacgtaca ttcatccgca aatgctgagt ctttgagttg cgtggaataa
      661 atttcagcga gttgttccag cgtgcgcacg gcaaagaaat tcgacgggat taggaatgga
      721 tagatgcaag catcatcgga tgggcggaag gcggaatgga taagtccatt cggtcgggtt
      781 gggttgccga aaccgcctag tggcacagtg tcgtgtgaga cggatgtgac gcgttgaaag
      841 cgatacggac cgggaccatc ccagcgttgt tgctcacgca ttgtttttac aattagatgt
      901 gtagctactt gccagtctgc gtcgaagatc gaggcgtcgc cgcaggtctt ccaataatgg
      961 tgggcgagtc gaaccgggta gcacagcgag tcaagctcgt attttctttc atgcaattct
     1021 tttcgcatgc tagtgaggtc ggtctcccag tcactgcctg tgggtccgtc gttgaaggca
     1081 ttggcgtacg ggtcgatcaa aatgcattgg gtctggcgat gaatcacccc ggcgatcagg
     1141 tcttttagct ttgcgtctgt attgataagc ggaacatact gccagacctg agccgtcgag
     1201 tcgcgcagcc acatggcttc gatatcccct gtgatgacaa aagtatcggg ctttccattg
     1261 atagtcgaat gtttaaccgt ggtatccaaa gtatttggaa agcaattctc aaacagccag
     1321 gcaagttcgg ggtctgcgat ggcttgagag accgttgcaa tcgtctcttc aaccgccgag
     1381 ctgacaaagt tacgcttttc aacgggtggt ctttgtgaaa cgaatgtgcc ggacttggag
     1441 atggatgtct ttggttgagg cataaaatta tttactcacc ttgcacagta ggggctaccc
     1501 aactatgttt tatagaaatt tttcgcaaag gtcgaccctt tcttgtagat tggcatgtag
     1561 gtaaaccctg acgggtcgcc cctacgacca aatctcgtaa ggtgacttat ttagttgacc
     1621 gacgaaatgt gatttgacgc aagatgcttg ctcccatcgg aactaacttg gatgatgttt
     1681 tggagcctat agtgatgctg agggtctccc acagattttc tgcgtggaag ggttgttgtt
     1741 caaagcgca
//
LOCUS       DAOREF010000733         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_59585_length_1742_cov_3.484884, whole genome shotgun sequence.
ACCESSION   DAOREF010000733 DAOREF010000000
VERSION     DAOREF010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59585_length_1742_cov_3.484884"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKI33_13635"
     CDS             complement(<1..685)
                     /locus_tag="PKI33_13635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM38105.1"
                     /translation="MMVDSYKPYISGITNYVEVNKQYLEKAGHEVFVFTFGDLEYEDT
                     EPNIVRSPGLPLADTGFYLSMRYSRQAKKLLQTMDVVHVHHPFLSGRLALRYCRPAGI
                     PIVFTNHSRYDLYAQAYLPAMPEEVSVGMLQAYMPPFCKAVDLVITPSAGMARVLREL
                     KVESDVEIVPNGVDLTSFQNAHPLEREAFGYKPDDILIVYAGRIALEKNLPFLIESFN
                     GVANAIKNVH"
     gene            833..1027
                     /locus_tag="PKI33_13640"
     CDS             833..1027
                     /locus_tag="PKI33_13640"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNM38106.1"
                     /translation="MPVKLILRSKEFEVKAGMALLDALKKCNIVPESVIATRDGELIT
                     EDELLKDGEVIKLVAVISGG"
     gene            1031..>1742
                     /locus_tag="PKI33_13645"
     CDS             1031..>1742
                     /locus_tag="PKI33_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HNM38107.1"
                     /translation="MKCKKCGEKASVNMRQHRMALCKEHFLEWIPEQTERFIKKYNMF
                     TRDEKVLVAVSGGKDSLALWDILIRLGYKADGLYLGLGIDGGVQYSDESHRLTEEFAR
                     KNNLTLHVVDIEKEYGHSIPVLSEISFRGKGKPCSVCGLTKRHEMNRIARDLGYDVLA
                     TGHNLDDEAAVLFGNTLQWSPDYLLRQGPVLQSKQGLARKVKPLCRFYEREMTAYSIA
                     RGIEYIYEECPFAEGATQI"
BASE COUNT          460 a          346 c          469 g          467 t
ORIGIN      
        1 agtggacatt ttttatagcg ttggcgactc cattaaagga ctcgataaga aatggcagat
       61 tcttttccaa cgcaattcgc ccggcgtaga cgatcagaat atcatcgggt ttgtagccaa
      121 aagcctcgcg ctcaagggga tgtgcatttt gaaagctagt gaggtcaaca ccattgggga
      181 caatctcaac gtcactttct actttgagtt cgcgtagcac tcttgccatg cctgccgaag
      241 gtgtgatgac aaggtcaact gctttgcaaa atggcggcat ataggcttgt aacattccaa
      301 cgctaacttc ttcaggcata gcagggagat atgcttgcgc atagaggtcg tagcgcgaat
      361 gattcgtaaa gacgatcgga attccagctg ggcggcaata ccgcaaggca aggcgcccac
      421 ttaagaaggg gtgatggaca tgcacaacgt ccatggtttg caagagcttt tttgcctgac
      481 gtgaataccg catggagaga taaaagcctg tatctgccaa tggtaagcca gggctgcgga
      541 cgatattggg ttcggtatct tcgtactcaa ggtcgccaaa agtaaagacg aacacttcat
      601 gcccggcttt ttcgaggtat tgcttgttga cctcaacgta attcgtgatg ccgctgatat
      661 acggtttgta gctgtcgacc atcattccta ttcgcataat atgatgctac ggcatgcagg
      721 tgggaatgtc aaggtgggtt ttgtgcatgg tagaataacc gtccttcaag ggtcgcagag
      781 atagtgatga aggtgtatta tcaatataag caaagcaaca ggagcgaacc ccatgcccgt
      841 caagttgatt ttaagaagta aagagtttga agttaaagcc ggaatggctt tgttagatgc
      901 gttgaaaaaa tgcaatattg tgcctgaatc tgtgattgcc actcgtgatg gtgagttgat
      961 tacagaagat gaacttctaa aagatgggga agttattaag ttggttgcag tgatttcagg
     1021 tggttaagcg atgaagtgta aaaagtgcgg tgagaaagct tcagtcaata tgcgccagca
     1081 tcgcatggcg ttgtgtaagg agcattttct agaatggatt cctgaacaaa ctgagcgttt
     1141 tatcaaaaaa tacaatatgt ttacgcgaga tgaaaaggtt cttgttgccg tctcaggcgg
     1201 caaggactcg ttggcgcttt gggatattct tatccgcctt ggctacaagg cagatggttt
     1261 gtatcttggg ttaggcattg atggtggtgt tcaatactcc gatgagtctc atcgcctgac
     1321 ggaagagttc gcgcgcaaga ataacctcac attgcatgtt gtggatattg aaaaagaata
     1381 cgggcattcc atccctgtgc tttcggagat cagttttcgc ggcaaaggca aaccttgttc
     1441 ggtatgcggg ctaaccaaac gtcatgaaat gaaccgcatc gcacgagacc tgggctatga
     1501 tgtgcttgca accggtcata acctggatga tgaagcggcg gtcttatttg ggaatacctt
     1561 gcagtggtct cctgattatc ttcttcgtca gggacctgtg ttgcagtcaa agcagggatt
     1621 ggcgcgtaag gtcaagccgt tgtgccgttt ttacgaacgc gaaatgacag catattccat
     1681 tgcgcgtggc attgaataca tttatgagga atgtccgttc gcggaagggg caactcagat
     1741 ct
//
LOCUS       DAOREF010000734         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_60181_length_1733_cov_2.648987, whole genome shotgun sequence.
ACCESSION   DAOREF010000734 DAOREF010000000
VERSION     DAOREF010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60181_length_1733_cov_2.648987"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKI33_13650"
     CDS             complement(<1..487)
                     /locus_tag="PKI33_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38108.1"
                     /translation="MNTTFSSDDENLKSQVSKELEEAKRLAKSFEFEKVKNGEWFITL
                     LKQVTKAYDRNARSAYFQKKYPGLSPDEIADILTSVTVRYATIAGAVAGVAASASEIA
                     SLGSAGMTATLFIGAIGAEMLYLSNIQMRLMLDMSVIYDLHLDPEDPEDILMVFGYAL
                     GV"
     gene            complement(755..>1733)
                     /locus_tag="PKI33_13655"
     CDS             complement(755..>1733)
                     /locus_tag="PKI33_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38109.1"
                     /translation="FNSTGKKLAKSDLVLAELATRIEGLTSKKMGKLIDEWKPNYQFT
                     RPFLIQCLAAVHTHRMDIKKPEDVWGDSTPKQIEKSWERTAEALDEVIEFLTGFIQWE
                     SSSWLPSFNALIPLVYINAYNRSFNAQEKRLARKWLLLAGVHAHFSGSVQTELDRILK
                     KLNSNVSVDQLWKITSKKLPKLRADNDLQVSRRSGPLMSLFISMLRNENSRDWISGNP
                     LNGTVLGKNAKLHVHHFFPRALLYKHNYSSEWINAFANYTVLCSGTNWNISTEEPLTY
                     LSRLKIHKNHLEAQCIPQDPSLWQVARYEDFIVEREKLLTKRLNEYLDT"
BASE COUNT          454 a          357 c          358 g          564 t
ORIGIN      
        1 ccacacccag cgcatatccg aaaaccatga gtatgtcttc ggggtcctcc ggatccaaat
       61 gcaagtcata tatcaccgac atgtccaaca taagccgcat ttgaatattg gagaggtaaa
      121 gcatttctgc gccgatggct ccgataaaca aggtggctgt catacctgct gaaccgaggg
      181 atgctatttc actggctgac gctgcaactc cggcgaccgc cccggcaatg gtggcatatc
      241 tgactgttac agaggttagg atatctgcaa tttcgtcagg ggatagtcct gggtattttt
      301 tctgaaaata agcagatctg gcattgcggt catatgcttt ggtgacctgt ttgagcaggg
      361 tgatgaacca ttcaccattt tttacttttt caaactcaaa cgattttgcc agtcttttcg
      421 cttcttcaag ttctttcgag acctgggatt ttaggttttc atcatctgat gagaatgtgg
      481 tgttcaatgt aaacctcctt gtagtatcta aattcgtact atatctttct ttactacttg
      541 atcaaaagac tttacccaag cagtgtattg aaaaatttga tcaccgtcgt tgcaagttcc
      601 accatgggct tgccgatatc gttgaaattc cgggtaactt gcgcaaaccc ttccacagtg
      661 gacttgcccc gttcttccct gggcttggat ttcttcgctt caacttgcag ggattcttga
      721 tggtttagta tttgatctcg gatttgattt ttttctaagt atcgaggtat tcgtttaatc
      781 tttttgttag caacttttct cgctcaacaa taaaatcctc atagcgagct acttgccaaa
      841 gagatggatc ttgaggtatg cactgagctt ccaaatgatt tttatgtatt tttagacgtg
      901 ataagtaagt tagcggctct tcagtggata tattccagtt agtacctgag cacaaaaccg
      961 tgtaattagc aaaagcattg atccattctg atgaataatt gtgtttgtaa agcaaagctc
     1021 ttgggaagaa gtgatggaca tgaagttttg cgtttttacc caacacagtt ccatttaatg
     1081 ggttgccact aatccaatcc cttgaatttt cattgcggag catggatata aaaagtgaca
     1141 taagaggtcc actccgacgt gatacctgca aatcattatc agctcttaat tttggaagtt
     1201 ttttagatgt gattttccat aattgatcaa cagagacgtt actatttagc tttttaagaa
     1261 ttcgatctaa ttcggtttga actgacccgc tgaaatgcgc atgtactcct gctaataata
     1321 accatttacg cgccaagcgt ttttcttgtg cattaaacga cctgttgtat gcatttatgt
     1381 aaaccaatgg gattagagca ttgaaagagg gtagccaact tgaagattcc cattgaataa
     1441 aacccgttag aaattcaatg acttcatcaa gtgcctctgc tgttcgctcc catgattttt
     1501 caatttgttt cggcgtggaa tctccccaga cgtcctctgg cttttttata tccattcgat
     1561 gagtatggac ggctgccagg cattgtatta agaatggacg agtgaattga taattgggtt
     1621 tccattcatc aatgagtttg cccattttct tagaagtcaa accctcaatt cgagtagcaa
     1681 gttcggcaag aactaaatcg cttttggcaa gctttttacc tgtggagttg aac
//
LOCUS       DAOREF010000735         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_60225_length_1732_cov_4.002385, whole genome shotgun sequence.
ACCESSION   DAOREF010000735 DAOREF010000000
VERSION     DAOREF010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60225_length_1732_cov_4.002385"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKI33_13660"
     CDS             complement(<1..260)
                     /locus_tag="PKI33_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38110.1"
                     /translation="MPFYHKLGKIPHKRHTQFKKPDGKLYREELMGLEGFSSLQSILY
                     HHFLPPRVKKTADLGSAKPEYVDYGAIRHRAFKTAPTPLGADP"
     gene            complement(270..1097)
                     /locus_tag="PKI33_13665"
     CDS             complement(270..1097)
                     /locus_tag="PKI33_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38111.1"
                     /translation="MKLHMLVTGISLVVLVGCAPTATATESPSPTPLAVEPSATNPPT
                     ETETVSPSAFEAENISTFNELGIAIGFNYPEGFSQSIGTSFNTTYSPVAPYDLPYPQN
                     AQIIFTDYSGGDGFTINGLRVFLADEVNALETGAVESVNAVLEGEADHHVDFPRLAGA
                     GSIIDAQVLPLAFQNGNGYRYLVAKSFSADPISSTKLTYMYQGVTNDGKFFVSFIMLV
                     DAPFLSEYVGQTLTTPEEFKTYFQDVNNLVETSSGDQFTPSLNALDELISSIVILEK"
     gene            complement(1111..1500)
                     /locus_tag="PKI33_13670"
     CDS             complement(1111..1500)
                     /locus_tag="PKI33_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNM38112.1"
                     /translation="MTETVITVRPQGTHLSKQQLPNFEGISAKTAGSKHLCMHLVVIP
                     PGGKAVAHYHDGYETVIYIIEGKAETRYGHNLEHSTINEAGDFLFIPPNLPHQPVNLS
                     DTEQVIAVVARNDPNEQESVVVYEVKE"
     gene            complement(1509..>1732)
                     /locus_tag="PKI33_13675"
     CDS             complement(1509..>1732)
                     /locus_tag="PKI33_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38113.1"
                     /translation="YMVAIMVPVTLPIQATAENPSADGVVYPSDISDTIALDAYFQGV
                     TDLLNAATPDSFQPNLNVLDALIQSIQIK"
BASE COUNT          434 a          384 c          430 g          484 t
ORIGIN      
        1 ggatccgccc cgagcggagt gggagccgtt ttgaacgcac gatggcggat cgctccgtaa
       61 tccacatatt caggtttggc agacccaaga tccgctgtct tttttacgcg cgggggcaga
      121 aagtgatgat agaggatcga ttgcagcgat gaaaatcctt cgagccccat cagctcttcg
      181 cgataaagtt ttccatctgg ttttttgaat tgtgtgtgac gtttgtgcgg aattttccca
      241 agtttgtgat agaacggcat gtcttctcct tatttttcta aaatgacaat cgaagaaatc
      301 aattcgtcca gcgcgttaag tgaaggagta aattggtcgc cgctggaggt ttctacaagg
      361 ttattcacat cttgaaaata agttttaaac tcttcgggtg ttgtaagagt ttgtccaaca
      421 tattcactaa gaaaaggtgc atcaactagc atgatgaacg acacaaaaaa ctttccatca
      481 tttgtaacgc cctgatacat ataagttaac ttcgtgctgc taattgggtc tgctgagaaa
      541 gactttgcca caagatagcg atagccattg ccattttgga atgccagagg gagcacttgc
      601 gcatcgataa tgctgcccgc acccgccaag cgcggaaagt caacatggtg atctgcttca
      661 ccttcaagga ccgcattcac actctccacg gctccggttt ccaaagcgtt aacttcatct
      721 gccagaaaaa cacgcagtcc atttatagtg aaaccatctc cacctgaata atcggtgaag
      781 atgatctgag cattttgagg atatggcagg tcgtaaggag caaccggcga ataggttgtg
      841 ttgaagctcg tgccgatgct ttgtgagaag ccctcaggat aattaaaacc gattgcgatg
      901 cctaactcat taaacgtgga aatattttcc gcctcaaacg cagacggtga aactgtttca
      961 gtttctgtag gtgggttcgt cgcagatggc tccacagcta gaggtgtcgg gcttgggctt
     1021 tcggttgccg ttgctgtcgg tgcgcaaccg accagaacta ccaatgaaat gcctgtaaca
     1081 agcatatgaa gtttcatttt cacctgcttt ctattctttt acttcatata ctacaacact
     1141 ttcctgctca tttgggtcgt ttctagcgac cacagctatg acttgttctg tatcgctaag
     1201 gttaacgggt tgatgcggta aatttggcgg gatgaaaaga aagtcgcccg cttcgttaat
     1261 tgtggagtgc tcaaggttat gcccatagcg cgtctcggct ttcccttcga tgatgtagat
     1321 cacagtctcg tagccatcat gatagtgagc taccgctttc ccacctggcg ggatgacgac
     1381 aaggtgcatg caaagatgtt tggaccctgc cgtcttcgca gagatgcctt caaaatttgg
     1441 taactgttgt ttgctgaggt gagtcccttg tgggcgaacg gtgatcactg tttcggtcat
     1501 gatgctcctt atttgatttg gatggattga atgagcgcgt ctaaaacgtt taaattcggc
     1561 tgaaatgaat ctggggtagc cgcatttagc aaatcagtga ccccttggaa gtatgcgtcc
     1621 agcgcgatgg tatctgaaat atcgctgggg taaacaacac catcggcaga ggggttttct
     1681 gcggtagcct gtatgggcaa tgtgacaggt accatgatgg caaccatata ct
//
LOCUS       DAOREF010000736         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_60430_length_1729_cov_3.321983, whole genome shotgun sequence.
ACCESSION   DAOREF010000736 DAOREF010000000
VERSION     DAOREF010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60430_length_1729_cov_3.321983"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKI33_13680"
     CDS             complement(<1..440)
                     /locus_tag="PKI33_13680"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM38114.1"
                     /translation="MNKDIKEPIEPILSKNEVRRSGETTFVAMRHRNYQLYFGGQLIS
                     NIGTWMQVIAQAWVVYQLGHSELTLGLVAFASAIPNLIVSPWGGVIVDRISRRKVLIM
                     TQSGAMVLSFILAWLTFTNIVQEWHVILLAALTGVITAFDAPAR"
     gene            complement(462..902)
                     /locus_tag="PKI33_13685"
     CDS             complement(462..902)
                     /locus_tag="PKI33_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNM38115.1"
                     /translation="MSTATQLREQALDGFWETIPPLWNFVRSYIRATATSKFDITVEQ
                     FHVLRFVRKGMSISELAIAKNISRPAISQAVDVLVKKGLLTRVQSTKDRRYVTLALTE
                     EGNALLDTVFKETRTWMKGRMSKLTNEELESIAKAMQAFKKILP"
     gene            1032..>1729
                     /locus_tag="PKI33_13690"
     CDS             1032..>1729
                     /locus_tag="PKI33_13690"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNM38116.1"
                     /translation="MTQKNNMHERPVEILQRLIQFNTTNPPGHEAACVGYIRDLLNEA
                     GIESTLVAKDPDRPNLIARLPGEGKAPPLLLYGHVDVVTTESQRWQQAPFEGKLIDGY
                     VWGRGALDMKGGVAMMVAAFMRANAEGLRPPGDIVLAIVSDEEAGGDYGAKFLVEEHP
                     ELFKDIKHAIGEFGGFTLTLGGKRFYPIMISEKQVCWMKATVRGQGGHGSMPEKGGAT
                     AKLAKFLKALDEKD"
BASE COUNT          448 a          392 c          446 g          443 t
ORIGIN      
        1 tgacgtgccg gggcatcaaa ggcggtaatc acacccgtca aggctgccag cagaatcaca
       61 tgccattcct gcacgatgtt ggtaaatgtc aaccaggcga ggatgaaaga caacaccatt
      121 gcaccagact gggtcattat caatacttta cggcgcgata tgcggtctac gatcacaccg
      181 ccccatggag agacgattaa gtttggaatg gcagaagcga aggcgaccaa ccccaacgtc
      241 aactcggagt gaccgagctg atacaccacc caagcctgtg caatgacctg catccacgtg
      301 ccgatgttgg agattaactg tccgccaaag tacaactgat aattacgatg ccgcatggca
      361 acaaaggttg tttccccgct tctacgtact tcattctttg aaagaatagg ctctatcggt
      421 tcttttatat ctttattcat tcttatgtca aactcctaaa gttagggcaa tatttttttg
      481 aaggcttgca tcgccttggc aatcgactcc aattcttcgt tggttaactt gctcatgcga
      541 cctttcatcc atgtgcgcgt ttctttgaaa actgtatcta acaaggcgtt gccttcttcg
      601 gtcagtgcaa gggtcacata tcgacgatct tttgtgcttt gcacgcgggt caataatcct
      661 tttttgacca aaacatctac agcctggcta atggcgggac ggctgatgtt tttggcaatt
      721 gccagctcac taatcgacat ccccttgcgc acaaaacgca gtacatggaa ctgttcaacc
      781 gtaatatcaa atttactggt ggcggtggct cgaatgtacg aacggacaaa attccaaaga
      841 gggggaatgg tttcccagaa gccgtccaag gcttgttcac ggagttgggt ggcagtgctc
      901 attgaaacct ttcaaattat gtaattttat taatagttaa gtgttgaaac tattttatgc
      961 ctgtttacga aaaacaacaa gaaggggatt aggcatataa tttggaagcc atctatagat
     1021 tggagctttc aatgacgcaa aaaaataata tgcatgaacg acccgttgaa attcttcaac
     1081 ggctgatcca gttcaacacg accaacccgc ccggtcacga ggcggcttgt gtaggctaca
     1141 tccgtgattt gttgaatgag gcgggcattg aatccacgct ggtagccaaa gacccagacc
     1201 gacctaattt gatcgcacgc ctgcccggtg agggtaaggc tccgcctttg ttgttgtatg
     1261 ggcatgtgga tgtggtgacg accgagagtc aacgctggca gcaagctcca tttgagggca
     1321 agctcatcga tggatatgta tgggggcgtg gcgctttaga tatgaagggc ggcgtggcaa
     1381 tgatggtggc ggctttcatg cgcgccaatg ctgaaggctt gcgcccgcct ggggatattg
     1441 tgctggcaat cgtcagcgat gaagaggcgg gtggcgatta cggcgcaaag tttcttgtgg
     1501 aggaacatcc tgagttattt aaagatatca agcatgccat cggtgaattt ggcggtttca
     1561 ctttaactct tggcggcaag cgcttttacc cgatcatgat ctctgaaaag caagtctgtt
     1621 ggatgaaagc caccgtgcgc ggacagggtg gtcacggttc gatgccagag aaaggcggcg
     1681 cgacagcgaa attggcgaag tttttaaaag cgttggatga aaaagactt
//
LOCUS       DAOREF010000737         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_60602_length_1726_cov_4.210652, whole genome shotgun sequence.
ACCESSION   DAOREF010000737 DAOREF010000000
VERSION     DAOREF010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60602_length_1726_cov_4.210652"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /gene="murG"
                     /locus_tag="PKI33_13695"
     CDS             <1..1117
                     /gene="murG"
                     /locus_tag="PKI33_13695"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNM38117.1"
                     /translation="MNGGRTVRLLICAGGTGGGVYPALSVYEALKSKHPNVETLWVGG
                     EGGMEEDLVKRAGIPYRSVPAAGVHGVGLRALPGNLAKLTRGVLESRRILKEFKPDVL
                     FFTGGYVAGPMAVAGRNLPIVLYVPDIEPGLALKFLGYFADVITVTAPDSKKFFSHPE
                     RIVHTGYPLRSGLLNWSREKAISHFQLDSSLPTLLVTGGSKGARSINMAVLKSLNELL
                     EMTQIIHLTGSLDIETAQQAAANLSAEKKLRYHALPYLHDMGAALSAADLVLSRAGAS
                     SIGEYPFFGLSAVLTPYPYAWRYQKVNADYLAERNAAIILQDELLNDNLLTVIKDVLN
                     NAQKRQAMRTAMKNLSMPNAADTIASQLVKLAGDETL"
     gene            1114..1662
                     /locus_tag="PKI33_13700"
     CDS             1114..1662
                     /locus_tag="PKI33_13700"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNM38118.1"
                     /translation="MMSAVYIFWMYVFLFGIIGAMRGWAKELMVIFSVVTSLAVNLLL
                     EKYIPLTRNLVDPNNPTQDALTAQFWIRTLILTALVYFGYQTVNISRFAPKAARERLQ
                     DSMFGAVLGGVNGYLVAGSVLYYNHVANYPFRNVISPATDAAIADAINRMMNVMPPRF
                     LAEPQIYFAVIIILIFIIVVYI"
BASE COUNT          383 a          440 c          434 g          469 t
ORIGIN      
        1 gatgaatgga ggtcgtacag tgcggttgct aatttgcgct ggtggaacgg gcgggggcgt
       61 gtatcccgct ctgtccgttt acgaagcgct aaagagtaag catccaaacg tcgaaactct
      121 ttgggttggc ggcgaaggcg gcatggaaga agacctggtg aaacgtgcag gcattccata
      181 ccgttctgtt cctgctgccg gagtgcatgg cgtgggtctg cgcgcattgc cgggcaacct
      241 tgccaaactc acccgcggcg tacttgaatc acgtcgtatt ctaaaagaat tcaaacccga
      301 tgttttgttt ttcaccggcg gctatgttgc gggtcccatg gcagtagcgg ggcgcaactt
      361 gcccatcgtt ctctacgtac cagatattga gccagggctt gcgctaaaat tcctaggcta
      421 ttttgcggat gtcatcaccg tgaccgcgcc ggactcaaag aagtttttct cacacccaga
      481 gcgcatcgtc cacaccggct acccgcttcg ttccggactt ttgaactggt ctcgcgaaaa
      541 agccatttct cacttccagc tggattcttc ccttcccacc ctcttggtga ctggcggtag
      601 caaaggcgca cgttcgatca acatggctgt gttgaaatct ttgaacgaat tactagagat
      661 gacgcagatc attcacctca ctggctcgtt ggatattgaa accgcacaac aagccgcagc
      721 gaatttatct gcagagaaaa agctccgcta tcatgccctg ccctatttgc atgatatggg
      781 tgcagccctc tccgccgcag acttggtctt gtctcgagct ggtgcctctt cgatagggga
      841 gtatcccttc tttgggttgt ctgccgtgct taccccctat ccgtatgcat ggcgctatca
      901 gaaggtcaat gctgattatt tggcagaaag aaacgctgcg attatcttgc aagacgaatt
      961 gttgaacgat aatctcttga cggtcattaa agatgtattg aacaatgcgc aaaagcgcca
     1021 agccatgcgt acagcgatga aaaatttatc catgcccaat gcggcagaca ccattgccag
     1081 ccagttggta aaactggcag gagacgaaac cttatgatga gtgctgttta tatattctgg
     1141 atgtatgttt tcctctttgg catcatcggc gccatgcgcg gatgggcgaa agaattgatg
     1201 gtcatcttca gcgtggtcac ttcgttggcg gttaacttgt tgttggaaaa atacattcca
     1261 ctgacccgca accttgtaga ccccaacaac ccaacccaag atgcgctcac cgcccagttt
     1321 tggattcgca ccttgatcct gacggcgttg gtgtactttg ggtatcaaac cgtgaatatc
     1381 tcacgctttg ccccgaaggc agcccgtgaa cgcttacaag actccatgtt cggtgcggtc
     1441 ttgggcggtg tgaacggata tttggtggct ggctctgttc tttattacaa ccatgtcgcg
     1501 aactatccct ttcgcaatgt gatcagcccg gcaaccgatg ccgcaattgc ggatgcgatc
     1561 aaccgtatga tgaatgtgat gccgccgcgc tttctcgcag aaccgcagat ctattttgcg
     1621 gtcatcatta ttttgatctt catcattgtt gtgtatatct aatactttct gctggattac
     1681 tttatgactc atgttcattt catcggcatt ggaggttcgg ggcttt
//
LOCUS       DAOREF010000738         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_60614_length_1726_cov_3.406942, whole genome shotgun sequence.
ACCESSION   DAOREF010000738 DAOREF010000000
VERSION     DAOREF010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60614_length_1726_cov_3.406942"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..650)
                     /locus_tag="PKI33_13705"
     CDS             complement(87..650)
                     /locus_tag="PKI33_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38119.1"
                     /translation="MNFLIGVTVTILAVLAFLEHPYYFRYHSLRFVYRSGLLLYSRTF
                     KIQTVANKTNPSRGHILSWMNDSGFSIPKAVEDGEKQYLIKEFLWHIGLRGDIHISAL
                     MRAKISWNNKENNVVVRDYVTWSYFSILVFAVAMLFIPSKVEGDTCGGVIFFICLLWF
                     AFLYVYQLPHLESVGKKVAEYLSSDEI"
     gene            complement(823..1650)
                     /locus_tag="PKI33_13710"
     CDS             complement(823..1650)
                     /locus_tag="PKI33_13710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM38120.1"
                     /translation="MIENNILINPTSNEIASAVHENLYALFRSMQVISNYELAENDRL
                     GLHHAPLSNPMFMGAWKANFSLDNVENEIDEVLSWFKKRNAPSFFWWTDIQTQPAGLV
                     EHLLKRGFDGNLEGDPGMAVNLHEINENIKLPNSLEIVWAKDNKTLVDWRNIFAEAFE
                     SPVAAGQAWVDATTSTSIENSPWQLYVGYLNQQPVSISILFNGAGVSGIYGVGTIPTH
                     RNKGIGAAMTLIPLLQARNQGYHFGVLFSSRMGYSVYKRLGFYEVGNNIGLFMKEWD"
BASE COUNT          519 a          372 c          341 g          494 t
ORIGIN      
        1 gcttgtaaat cgcgcagact cccacacgtc gggtgcacgc tttgttagcc agccgtgttt
       61 tgcgcaacac ttcttttttt gtttcttcat atttcatctg aagacaaata ctctgcaact
      121 tttttgccaa cagattcaag atgtggcagt tgatatacat aaagaaaagc aaaccacaaa
      181 agacagataa aaaatattac gccaccgcac gtatcccctt caacctttga aggaatgaac
      241 aacatcgcaa ctgcaaatac caatattgaa aaataagacc atgttacata atcgcgaaca
      301 acgacattgt tttctttgtt gttccaagag attttagcac gcattagcgc ggaaatatga
      361 atatcccctc ttaagccaat gtgccaaaga aattccttta tcaaatactg cttttcacca
      421 tcttccactg ccttcgggat tgaaaaaccc gaatcgttca tccaagacaa aatatgccca
      481 cgagaaggat tcgttttgtt tgccacggtc tgaattttga aggtacggga atataagagc
      541 agaccgcttc tgtaaacaaa acgcaaagaa tggtatcgga aataataagg atgttccaaa
      601 aaagccaaaa cagcgagtat tgttactgtt acgccgataa gaaagttcat tggattttaa
      661 tttccgataa tttgataggc aagccaacgg attgcgttac ctgcgctggg gtggggacgg
      721 cgaagccgtc caaccagaaa aatgataagg cgtaggaaac tgcttgagat tgccgcagaa
      781 tccccagcgt caggtgcacg ctttgttggg cgtttttgat catcaatccc attctttcat
      841 aaacaagcca atattatttc cgacttcata aaaccctaac cgcttataaa cagaatagcc
      901 cattcgcgat gaaaacaaga cgccaaaatg gtatccctga tttctggctt gaagtaatgg
      961 aattaatgtc attgctgcac caatgccttt gttgcgatgt gttggtatgg tgcctactcc
     1021 atatattcct gatactccag cgccattaaa gaggatactg attgatactg gttgctggtt
     1081 aagataacca acatacaatt gccaaggcga attttctatg gaagtagatg ttgtcgcgtc
     1141 tacccatgct tgtccagccg caactgggct ttcaaatgct tcagcgaaaa tgttgcgcca
     1201 atccaccaat gttttattat cctttgccca aacaatctca agactgtttg gtaatttgat
     1261 attttcattg atttcatgaa gattgacagc cataccaggg tcgccttcta aattgccgtc
     1321 gaaaccccgt tttagcaaat gttcaactaa cccagcaggc tgagtttgaa tatcagtcca
     1381 ccaaaaaaaa gatggtgcat tgcgtttctt gaaccaagac aaaacttcat caatttcatt
     1441 ttcaacattg tctaaagaaa aatttgcttt ccatgcaccc atgaacatag gattagaaag
     1501 tggtgcatga tgcaacccaa gacgatcatt ttctgccaat tcgtaatttg atatgacttg
     1561 catggagcgg aaaagagcat acaagttttc atggacggca gatgcgattt cattacttgt
     1621 tggattaata agaatattgt tttcgatcat gatatctgct ttcttttcaa cgcccaacgg
     1681 tttgcgttac ctgcgtgtgg gcggagtgtg gattctgctt gggagc
//
LOCUS       DAOREF010000739         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_60867_length_1722_cov_3.830234, whole genome shotgun sequence.
ACCESSION   DAOREF010000739 DAOREF010000000
VERSION     DAOREF010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60867_length_1722_cov_3.830234"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1533
                     /locus_tag="PKI33_13715"
     CDS             55..1533
                     /locus_tag="PKI33_13715"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNM38121.1"
                     /translation="MTTKLNETLGKLKNVPILSSLPNLPKKVIWVIIVSIVVLAGAGG
                     GYAYYQNTQTADESTSTSELQTAMATRGDLVIYASGTGTLVSANETELSFTSTGKVTE
                     VNVTVGEKVEAGTQLAKLDDTDAQATYAEANRAYLELTSPSAIATAQQSIADATSDVS
                     DAKNTLAYIISPEALQAEEKVEEAQTAIAEAEAKLKESPTDTDAKKALEDAQSDLEAS
                     QELLAQAQGRYTSYYLPKYFTDGKELNAPSEAEILAARADYELAKATLQEAKWLYAAL
                     TGEEIPENATGNGLTELEQARLNLESAKATLDGTQIIAPYAGTVMSIDISVGDSVSSG
                     SSAITLADLTQPYLEVYLDESDWSSVVVGYETDVIFDILPDKTFSGVVTQVDPGLYSS
                     NNTSVVRAFVKLTQIDEESFTLPLGTTASVDVIGGRAENAVLVPIEALHKAGDQYAVF
                     IMENDKPVLHVVEVGIQDLLYAEVISGVKEGDLVTTGIVETQ"
     gene            1530..>1722
                     /locus_tag="PKI33_13720"
     CDS             1530..>1722
                     /locus_tag="PKI33_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM38122.1"
                     /translation="MSTEHSIIETVDITKVYGMGDISVSALRGVSIQIQKGEFVAIMG
                     PSGSGKSTLMHILGCLSKPS"
BASE COUNT          508 a          378 c          412 g          424 t
ORIGIN      
        1 tttttatatt gaaagagaaa tatctgatct catttttaca gggaaattaa gcccatgaca
       61 acaaaattaa atgaaacact aggaaagtta aaaaatgttc ccatcctttc ctctttacca
      121 aacctaccga aaaaggtgat ttgggtcatc atcgtcagca tagtggtatt agcaggcgct
      181 ggcggaggat atgcttatta tcaaaacacc caaactgcgg atgaaagcac cagcacttct
      241 gaattgcaaa ccgctatggc aaccagaggt gaccttgtca tttatgccag tggcacaggc
      301 actttagtct ctgcaaatga aaccgagctt tcctttacgt ctaccggcaa agtgaccgag
      361 gttaatgtga ccgttggtga aaaggtggaa gcaggcactc aactggcaaa gctcgatgat
      421 acagacgccc aagcaacata tgcggaagca aaccgagctt atttagagtt aacttctccc
      481 agcgcaatcg ctactgctca acaatccatt gcagatgcga ctagtgatgt cagcgatgca
      541 aaaaacaccc tagcttatat tattagccca gaagcgcttc aggcggagga aaaggttgaa
      601 gaagcgcaaa cagctatcgc tgaggctgag gcgaagctca aagagtcacc cacagacaca
      661 gacgcgaaaa aagccttaga ggacgctcag tctgaccttg aagcatccca agaactgttg
      721 gcacaggcgc aaggacgtta cacaagttat taccttccca aatatttcac agatggcaaa
      781 gaattaaatg caccttcaga ggctgagatt cttgccgcac gtgcagacta tgaacttgcc
      841 aaggcgaccc tacaagaagc taagtggctg tatgcagcgt tgaccggcga ggaaattcct
      901 gaaaatgcaa caggcaacgg cttgaccgag cttgagcaag cacgcttgaa cctcgaatct
      961 gcaaaagcga ctttagacgg tacgcagatc attgcaccat atgcaggtac ggttatgtcc
     1021 attgacatat cggttggaga tagtgtgagc agcggcagtt ctgccattac gcttgcggat
     1081 ctaacccagc catatttgga agtgtattta gatgaatcag attggtcgag cgttgtggtg
     1141 ggctatgaga cagatgttat tttcgatatt ttgcctgata aaacgttcag cggagttgta
     1201 acacaggtag accctgggct gtactcctcc aacaatactt ctgtagttcg ggctttcgta
     1261 aagttgactc aaattgatga agaaagcttc actttacctt taggcacaac cgcttctgtt
     1321 gatgttatcg gcgggcgggc tgaaaatgca gtgttggtgc ccatcgaagc cttgcacaaa
     1381 gcaggagacc aatatgccgt ttttattatg gaaaatgaca aacccgtttt gcatgtagtt
     1441 gaagtaggta ttcaagattt gctttatgcg gaggttattt caggcgtaaa agaaggcgac
     1501 ctggtaacaa ccggcatcgt ggagacgcaa tgagcacaga acattcgatt attgaaaccg
     1561 tcgacattac caaagtttat ggcatgggtg atattagcgt ctctgcctta cgaggcgtga
     1621 gcatccaaat tcaaaaaggc gaatttgtag ccattatggg tccttcgggc tctggcaaaa
     1681 gtacgctgat gcatatcctt ggctgtcttt caaagccatc gt
//
LOCUS       DAOREF010000740         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_61015_length_1720_cov_4.498498, whole genome shotgun sequence.
ACCESSION   DAOREF010000740 DAOREF010000000
VERSION     DAOREF010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61015_length_1720_cov_4.498498"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1050)
                     /locus_tag="PKI33_13725"
     CDS             complement(61..1050)
                     /locus_tag="PKI33_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNM38123.1"
                     /translation="MRKQTLTLLSVLTVLSMLLVACGATATEAPATEAAPTEEPLSGT
                     LSISGAFALYPMMTVWAEEFTKLHPDVQFDVQGGGAGKGMTDTIAGAVDIGMISRSIK
                     PEEEAQNIFWVSVTKDAVFPIINAENPVAAQILEKGISQEVFAKIYITGEITTWGEVV
                     GDPSITDKINVFTRSDSSGAAEQWSKYAGGEAQEDLLGIGVNGEPSMLDTVLKDPLAI
                     GFGNLNSIFDLSGGGLVPGIVIPPIDINSNGAVDAGELYKVKEDAFGAVADGTYPSPP
                     SRFENLATLGKPEGLELEFIKWILTDGQQYLEAAGFVPLTPEQQAESLAKLEQ"
     gene            1299..1703
                     /locus_tag="PKI33_13730"
     CDS             1299..1703
                     /locus_tag="PKI33_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNM38124.1"
                     /translation="MKLTARSEYALLALVSLARHESDGFVSIDTIATAQGIPPKFLEQ
                     LMLAVKRAHFLRSAKGQKGGYQLAKRPEQIPLADVIRLFDGALAPTESVSENFYESTP
                     IEKEKKLTKVFKDVRDYVSNKLEKTTIADVTG"
BASE COUNT          414 a          425 c          428 g          453 t
ORIGIN      
        1 gcgtagaagg tcggcttagc cgaccttctg ttttcaaagt gttgttgagt tgaatgtcac
       61 tcattgttcg agtttcgcaa gagactcggc ttgttgctct ggcgtgagcg gcacaaagcc
      121 tgccgcttcg agatattgct gaccgtccgt gagaatccac ttgatgaatt cgagttccag
      181 tccctcgggt ttgccgagcg tggcgaggtt ttcaaagcgc gaaggaggag aaggatacgt
      241 tccatctgcc actgcaccga aggcatcttc tttcaccttg taaagttcgc ctgcatccac
      301 agctccgttg gagttgatat caatcggcgg gatgacgatt ccaggcacaa gtccaccgcc
      361 gctcagatcg aaaatgctgt tgaggttacc gaagccaatg gcaagcggat ctttcaaaac
      421 tgtatcaagc atggaaggtt caccgtttac accgatacca agcagatctt cctgcgcttc
      481 gccgcccgca tacttggacc attgttcggc agccccagaa gaatccgaac gggtgaacac
      541 attgatctta tccgtgatgg atgggtcgcc aaccacttcg ccccaggtgg taatttcgcc
      601 ggtgatatag atcttggcaa agacttcctg agaaattccc ttttcgagaa tttgcgccgc
      661 gaccgggttt tcggcattga tgatcgggaa gaccgcgtct ttcgtcaccg acacccagaa
      721 gatattctgc gcttcttcct ctggcttgat gctgcgtgag atcatgccga tgtctacagc
      781 gcctgcgatg gtatcggtca tgcctttgcc agcgccgccg ccttgtacgt caaactgaac
      841 atcagggtgc agtttggtga attcttctgc ccaaacggtc atcatagggt agagggcgaa
      901 cgcacctgag atggacaatg tgccactgag gggttcttct gtgggagcgg cttcagtggc
      961 tggcgcttct gtcgcagttg caccacaagc gaccagaagc atggataaaa cggtcagtac
     1021 ggatagcaat gttaaggttt gttttcgcat attgtttctc caatttattt ggtttggtta
     1081 taactcctat tgaattagta gggatttaag gtaaataaaa agcgcttttg ttttcgagac
     1141 ctcgaatatc tctataaatc ctactagtct aataggttat gtttggttat cataattcag
     1201 ccactgaatt ttgtcaatac tcgaacaccc aacttgacag actgtttttt ttcactatac
     1261 tactccagca aaccaataaa agaagttgag attcgatcat gaagttgacc gcccgtagtg
     1321 agtacgccct gctcgccctt gtttccctcg cccgccatga gtcggacgga tttgtttcca
     1381 ttgataccat tgccacagca caaggtatcc cgcccaagtt tttggagcaa ctcatgcttg
     1441 cggtcaaacg cgcccacttc ctgcgcagcg ccaagggaca gaaaggcgga tatcaactgg
     1501 cgaagcggcc cgaacaaatt ccgcttgccg atgtgatccg tttatttgat ggcgcccttg
     1561 cccctactga atcggtcagt gagaattttt atgagtccac gccgattgag aaagaaaaga
     1621 aactcaccaa ggtcttcaag gatgtccggg attatgtctc gaacaaactg gagaagacca
     1681 cgatcgcgga tgtgaccggt taaagactgg taagaaaatt
//
LOCUS       DAOREF010000741         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_61084_length_1719_cov_4.763221, whole genome shotgun sequence.
ACCESSION   DAOREF010000741 DAOREF010000000
VERSION     DAOREF010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61084_length_1719_cov_4.763221"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKI33_13735"
     CDS             complement(<1..455)
                     /locus_tag="PKI33_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM38125.1"
                     /translation="MTNNIERHAKLLKASARAAKNITTILDSYELFQRTVDIICDEFG
                     FYYAGVFLVDDARQYAVLKAGRGDAGKAMLREEHKLAIGGNSMIGASIANKKGRIALD
                     VGEEAVFFENPHLPKTRSEMALPLIIADEVIGALTVQSTEEAAFHEEDI"
     gene            complement(478..864)
                     /locus_tag="PKI33_13740"
     CDS             complement(478..864)
                     /locus_tag="PKI33_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM38126.1"
                     /translation="MTTVLLVDDEKLIQRSLEKTLLRAGYDVLTAPDCKTGSEVFAQN
                     ASTVDIAVLDLNMPGFDGMPKNDAGLDLLADLKQKKSTLPVVILTAYDEVNKAKDAVS
                     KGANAFFVKGREQGLVELIQKILSGN"
     gene            complement(889..>1719)
                     /locus_tag="PKI33_13745"
     CDS             complement(889..>1719)
                     /locus_tag="PKI33_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM38127.1"
                     /translation="EAIHWVGNKAAPIPGSVHRVREDVQYLLALVAQLKESELDNAAL
                     QAAVETVREEAEAAGLDLKNILAEMSAMKPNRLQALVSVESLMEDLDIAENSAKTILD
                     IKEGLIGPARQRHDAPVSLSEMITNTVDNMGLPHGAVEMKWAEDMPMAHVDARQVEQV
                     FNNLIKNAWEALTLSNIPEPKIYVDGRCDKDANFVLVSVKDNGPGIPKEIQEKIWVSF
                     FTTKGGKGGTGLGLSAVMQIVNQHGGKIWLESEPGQGATFYVSLPAEKLYMPDENTSR
                     "
BASE COUNT          432 a          475 c          340 g          472 t
ORIGIN      
        1 tcgatatctt cttcgtggaa agcagcttct tcagttgatt gaaccgtgag ggctccgatc
       61 acctcgtctg caataataag cggcagtgcc atttcagagc gggtctttgg caaatgcgga
      121 ttttcaaaga agaccgcttc ttcacccaca tccaatgcga tgcgtccttt tttattggcg
      181 atgctcgcac cgatcatgga gtttccgccg attgccagct tgtgctcttc acgcaacatg
      241 gctttcccgg catcaccacg cccagctttg agcacggcat actgacgcgc gtcatctacg
      301 aggaagacgc cggcatagta aaagccgaac tcatcgcaga tgatatccac agtgcgctgg
      361 aaaagctcgt aggagtcgag aatggtggtg atgttctttg cggcacgcgc ggaggctttc
      421 aataattttg catgtcgttc aatattattt gtcataagtt cctctgttga aagtgaatta
      481 atttccactc aagatttttt ggatcaactc caccaaacct tgctcacgtc cttttacgaa
      541 aaaggcattg gcacctttag aaacagcatc tttggctttg ttcacttcat cgtatgccgt
      601 caaaatcaca acgggtaatg ttgatttctt ttgcttgaga tctgccaaaa ggtcaagccc
      661 ggcatcattt ttaggcatac catcaaaacc gggcatattc aaatccagca cggcaatgtc
      721 aacggtgctg gcgttttgag caaagacctc actgcctgtc ttacaatcgg gcgcagtcaa
      781 cacatcatac ccagcccgca agagagtctt ttccaaacta cgttggatca acttctcatc
      841 gtccactaaa agaactgtcg tcatcattcc tccaagtttt tacacgcatt agcgtgaagt
      901 attctcatct ggcatatata acttttccgc aggcaggctc acataaaatg tagcaccttg
      961 cccgggttca ctttccaacc aaatcttgcc accatgttga ttgacaatct gcatcactgc
     1021 cgaaagcccc aagcccgtgc cacctttacc gcctttggtt gtaaagaacg acacccaaat
     1081 tttctcctgt atctctttcg gaattcccgg tccattatct tttacagaaa cgagcacaaa
     1141 atttgcatct ttatcacagc gcccatcaac ataaatttta ggctccggta tatttgagag
     1201 cgtcaacgct tcccaagcat ttttgatgag gttattgaaa acctgttcca cctggcgcgc
     1261 atccacatgt gccatgggca tatcttctgc ccatttcatt tccacggcgc catgcggcaa
     1321 gcccatatta tccaccgtgt tcgtaatcat ctcgctcaaa gaaaccggcg catcatgtcg
     1381 ctgacgagca ggtccgatca aaccttcttt gatatcaagg atggtctttg cgctattttc
     1441 ggcaatatct aaatcttcca tcaaagactc aacgctgacc aacgcctgca agcgattcgg
     1501 cttcatggca gacatttctg ccagaatatt tttcaaatcc aagcctgccg cttccgcctc
     1561 ttcccggacc gtttcaacgg cggcttgcaa cgcggcatta tccaactctg attcctttaa
     1621 ctgcgcaacc aaagccaata aatattgaac atcttcacgg acacgatgca cacttccagg
     1681 aatcggcgca gccttgttcc caacccagtg aattgcttc
//
LOCUS       DAOREF010000742         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_61421_length_1714_cov_4.192887, whole genome shotgun sequence.
ACCESSION   DAOREF010000742 DAOREF010000000
VERSION     DAOREF010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61421_length_1714_cov_4.192887"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKI33_13750"
     CDS             complement(<1..920)
                     /locus_tag="PKI33_13750"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNM38128.1"
                     /translation="MAIVSSVGTAQALDGREAGLQATHQALNKLGANVPGLAVVIASH
                     QYQAREVLNGVSSLLGDTPMIGFSSPAGLTRDGLHPHSVVVALLSGDFQAETLWLPGY
                     AQSGRETAQKIEQHASARVERQSVLFFADGFNGDAEQFCNSLPQALDVTGALSSGDLH
                     TGNTYQLMGAQTGAGCMTTAFLRGNLHIGVGSDHGWDPVGNQFRVTRSRGFWLRTLDG
                     RPASEAYAGLFGQPARDWAFPPLSYLARLYPLGMEQSDGMVVRAPIRVEADGSFRMNA
                     VIRDGVDAFLLVGSRASCERAAKTAAQHALL"
     gene            complement(927..>1714)
                     /locus_tag="PKI33_13755"
     CDS             complement(927..>1714)
                     /locus_tag="PKI33_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM38129.1"
                     /translation="RLTDHAAIAISNAQLYSEVQRANLAKSDFVSFVAHELKNPMTSI
                     KGYTELLASGAVGQITEMQTNFLGTIKSNVERMSTLVSDLNDNSKIEAGRLRLEFKAT
                     QASDLVDEVIRTFKRQLEDKKQTLELSIPPNLPAMWADRIRVGQVLTNLVSNAFKYTP
                     ESGAIQVGVEESPNHWDANGAPRVIHLWVKDSGIGMTPEDQQKIFQKFFRSDDPKARE
                     APGTGLGLNITKSLVEMQGGQIWFESEYRHGTTFHFTVPVAEE"
BASE COUNT          420 a          475 c          427 g          392 t
ORIGIN      
        1 agaagggcgt gttgagctgc tgttttggca gcacgttcgc aagaggcgcg gctccccacc
       61 aataagaaag cgtccacgcc atcacgaatc accgcgttca tgcggaagct accatcggct
      121 tccactcgaa tcggcgcacg cacaaccatg ccgtcgcttt gctccatacc aagcggatac
      181 aaacgcgcaa gatagctcaa tggtgggaat gcccaatcgc gagcaggttg cccgaacaaa
      241 ccagcatacg cctcagaagc cggacgacca tctaaagtgc gaagccagaa accgcgtgaa
      301 cgagtgacac ggaattgatt cccaacgggg tcccaaccgt ggtctgagcc gacaccgata
      361 tgaaggttgc cacgcaagaa agcagtggtc atacaaccag cgccggtttg cgcgcccatc
      421 aattgatagg tattgcccgt gtgtaaatcg ccgcttgaaa gcgcgccggt cacatccaaa
      481 gcttgtggaa gtgaattaca aaactgttct gcatcgccat tgaaaccgtc tgcaaagaaa
      541 agcacagact gacgctccac gcgcgcagaa gcgtgttgtt cgattttttg agccgtctca
      601 cgcccagatt gagcataccc cggcaaccaa agcgtctctg cctgaaaatc accgctgagt
      661 aatgccacaa ccaccgagtg tggatgaagc ccatcacgcg tcaatccggc aggagagcta
      721 aacccgatca tcggtgtatc acctaacaaa ctggatacgc cattcaacac ttcacgcgct
      781 tgatattgat gagaggcgat caccactgcc agcccgggga cattcgcccc aagcttgttc
      841 aacgcctgat gtgttgcctg caacccggct tcgcgtccat ccaacgcctg agcggttcca
      901 acagaagaga ctatcgccat cgctgattac tcctctgcca ctggcacagt gaaatggaag
      961 gttgtgccgt gtcgatattc actctcgaac caaatctgcc cgccctgcat ttctacaagg
     1021 cttttggtaa tgttcaaacc gagacctgtt cctggcgctt cgcgagcctt ggggtcatcg
     1081 gaacggaaga atttctggaa gatcttctgc tgatcttcag gtgtcatacc aataccactg
     1141 tctttcaccc acaaatggat aacacgtgga gccccattgg cgtcccaatg attcggactt
     1201 tcttctacac caacttgaat ggcgccgctt tctggtgtgt atttgaaggc attgctaaca
     1261 aggttggtca acacctgtcc cacgcggatg cgatctgccc acatggcagg caagtttggt
     1321 gggatgctta attcaagggt ttgcttctta tcttcaagtt gacgtttaaa ggtgcgaatg
     1381 acttcatcca ccaaatcaga agcttgtgtg gctttgaatt ccaagcgtag gcgacccgct
     1441 tcgattttgg aattatcgtt gaggtccgat accaatgtgg acatacgctc cacattcgat
     1501 ttgatcgtac cgaggaagtt cgtctgcatt tcagtgatct gccccactgc cccacttgcc
     1561 aacaactcgg tgtagccctt aatcgaggtc atcgggttct taagctcatg tgctacgaaa
     1621 gagacgaagt cacttttggc gaggttggca cgttgcactt cgctatacag ttgagcattt
     1681 gagatcgcga tagcggcatg gtcggtgagg cggg
//
LOCUS       DAOREF010000743         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_61614_length_1712_cov_2.118286, whole genome shotgun sequence.
ACCESSION   DAOREF010000743 DAOREF010000000
VERSION     DAOREF010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61614_length_1712_cov_2.118286"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1712
                     /locus_tag="PKI33_13760"
     CDS             <1..>1712
                     /locus_tag="PKI33_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNM38130.1"
                     /translation="TVAGFVNDLSRLPAEISLAGIGYITPETANELELEPEYNQLLIR
                     YADGSTRTEIEKLTTTVTEAVEEDEYIVFNANVPVPDTYILGDNMTSVLFILNSIGFL
                     TLILSAFLVTSVMSAMMSQQIPQIGILKSLGAHVGQTMSIYFREVLLFGIFALILAIP
                     MGLVGAYFLADGLASSMNFDVTNFYLPTQPLILQAISALLAPLLAALIPIISGSRITI
                     REALSNYTPDSASNALSLGEIPQIANLSIRNTFRRKSRLALTFAALLLAGSMFISIIG
                     IREEMRQASIEMQKEANYDLDVDLARPYSQSTLEETALRVKGVEEVETWAVDTGRLIF
                     SKDHLSGSIILYGIPQGTTMTQPAVINGTWLSKPKSIFVNNDFLLLSPDLKVGSVITL
                     NIGGKEEEWTIVGSTGRGFIPIAYVYYDDLTNVTGLDGFASRLVVRTDKSDSAFQTSA
                     QSDVLSHIAAEDIDVVNSSTTTERNETSLEQMDTLIILLMAMVILIAIVGGLGLAITM
                     SLNVFERTREIGILRSLGAQNGVVRRVVMIEGLVIGVISWVISIPCSVPLSIFLGNSL
                     GLSL"
BASE COUNT          400 a          473 c          388 g          451 t
ORIGIN      
        1 tgaccgttgc cggttttgta aatgaccttt ctcgtttacc tgctgagatc agcctggcag
       61 ggattggtta catcacccca gaaacagcca acgaacttga actcgagccg gaatacaatc
      121 agctgctcat ccgatatgca gatggctcta cccgcaccga aattgaaaaa ctgacgacca
      181 cagtgaccga agctgtcgaa gaggatgagt acatcgtttt caacgcgaat gtgcccgtgc
      241 ccgatactta catcctcggc gataacatga catcggttct tttcattttg aattcgattg
      301 gcttcctcac actgatcctg agcgcgttcc tcgtcaccag tgtgatgtct gccatgatgt
      361 cacagcagat ccctcagatc ggtattctaa aatctttagg tgcgcatgtc ggtcagacca
      421 tgtccatcta ttttcgagaa gtgctgctgt tcggcatctt cgccttgatc ctcgctattc
      481 caatgggctt ggtcggcgct tatttccttg cagatggtct ggcttcgagc atgaacttcg
      541 atgtgacaaa tttctacctg cccactcaac ccctgatctt gcaagccatc agtgcattgc
      601 ttgcgccatt attggcggcg ctcatcccta tcatttctgg ttcgcgcatc acgatccgtg
      661 aagcgctcag caactacact cccgatagtg cctccaatgc attaagcctt ggagagattc
      721 cgcagatcgc aaacctttcg attcgtaata ccttccgccg caaaagccgc cttgcactca
      781 ccttcgccgc gctgcttctg gctggttcca tgttcatttc cattatcggc attcgcgaag
      841 aaatgcggca agccagtatt gaaatgcaaa aagaagcgaa ttacgatctc gatgtagact
      901 tggcgcgccc atattctcaa agcacgttgg aagaaactgc cttgcgtgta aaaggtgtag
      961 aagaagttga aacctgggca gtggatacag gtcggctcat tttcagtaaa gaccacctga
     1021 gcggcagcat cattttgtat ggcattccac aaggcacaac catgacccaa cccgcagtca
     1081 tcaacggcac atggttatcc aaacccaaaa gcatcttcgt caacaacgat tttcttttgc
     1141 tctcccccga tctaaaagtt ggctctgtca ttacgctgaa cattggcggc aaagaagaag
     1201 aatggaccat cgttggttcc accgggcgcg gatttatccc catcgcttat gtgtactacg
     1261 atgacttgac caacgtcacc gggctggatg gcttcgccag ccgcctcgta gtgcggaccg
     1321 ataaatctga ttcagccttt caaacctcag ctcaatctga cgttctctcc cacatagccg
     1381 ctgaagatat agatgttgtc aattcatcca ccaccaccga acgaaatgaa acctcactcg
     1441 aacagatgga tactctgatc atccttctga tggcaatggt gattctgatc gccatcgtcg
     1501 gcggactggg gcttgccatc accatgagtc tcaatgtgtt cgaacgcacc cgcgagatcg
     1561 gtatattgcg ttcactgggc gctcaaaatg gagtcgttcg ccgggtcgtg atgatcgaag
     1621 gcttggtgat cggtgtcatc agttgggtga tctccatccc ctgctcagtt cccctctcta
     1681 tatttttggg taattccctc gggctttcgc tg
//
LOCUS       DAOREF010000744         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_61721_length_1710_cov_3.402417, whole genome shotgun sequence.
ACCESSION   DAOREF010000744 DAOREF010000000
VERSION     DAOREF010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61721_length_1710_cov_3.402417"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..831
                     /locus_tag="PKI33_13765"
     CDS             151..831
                     /locus_tag="PKI33_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM38131.1"
                     /translation="MSAELILLVDDEPSITELARMYLERDGYRIHEAADGESALETAE
                     KQKPALIVLDVMLPKVDGFDVCRRLRSAGNDVPIIMLTARDEDIDKILGLELGADDYL
                     TKPFNPRELVARVKAILRRSESKKDSDGKPIHRGDLTIDPVSREARLASRTLDLRAQE
                     FDLLLTLAESPGRVFSREQLLQLAWGFDYYGQTRTVDVHIAHLRKKLEGGNIKIETVT
                     GVGYKFVT"
     gene            867..>1710
                     /locus_tag="PKI33_13770"
     CDS             867..>1710
                     /locus_tag="PKI33_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM38132.1"
                     /translation="MLSSLRSRLWLSYTFLIVTALSVVTIVLFASLFRNPLLYRQTTE
                     RLNAVRSVMVTRLSEADAPPISTLAMRNAQTFNVRVAVFSQNRELVFDTSTEAELIFP
                     TVKRLTRALPLTRDTNGAAWLYSVEKLPDGTFLMVATPRPRNSVLNIFSDEFIPVIVL
                     GGGSALLLSLILAFLVSGWIASPLQKVVNAARGMPSAEVKPLEATGPHEVQELTRAFN
                     SMMARVLASQRSQREFVANVSHEMKTPLTSIQGFAQALLDGTANTKESQQKAAQVIYS
                     ESERM"
BASE COUNT          395 a          421 c          439 g          455 t
ORIGIN      
        1 gtctttctta agactatgga tgattttaca aaaatgaatg ctgtctatgc agaaatcttt
       61 ggtgaaaacc cacctgctcg tactaccgta gcagtttctg gtttaccaaa gaatgccttg
      121 gttgagatcg agtgcgttgc cctagcataa atgtctgctg aattaattct actcgtcgat
      181 gacgagccct ccattactga acttgcgcgc atgtatcttg agcgtgatgg atatcgcatt
      241 cacgaagccg ccgatggcga gtctgcgtta gagacggcag agaaacaaaa gccggctctt
      301 attgtattgg atgtgatgct ccccaaagta gatggctttg atgtctgtcg tcgattacgt
      361 tctgcgggga acgatgttcc catcattatg ctcaccgctc gggatgaaga tatcgacaaa
      421 attcttgggc ttgaacttgg cgcagatgat taccttacca aaccgttcaa cccacgagag
      481 ttggtggcgc gtgttaaagc cattttgcgc cgtagtgaat ccaaaaagga ttcggacggt
      541 aaaccgatcc accgcggtga cttgacgatt gaccctgttt ctcgagaagc ccgcctcgct
      601 tcgcggacct tagacctgcg cgcccaagaa tttgaccttt tactcactct ggcagagtcg
      661 ccggggcgtg tcttctcgcg tgaacaatta ctgcaactcg cctggggctt tgactattat
      721 ggtcagaccc gcaccgtgga tgttcacatt gcacaccttc gcaagaaact cgaaggtggc
      781 aatatcaaga ttgaaaccgt cacaggagta gggtacaaat ttgtcactta agatacgtgt
      841 tgcgtaattt gtaaaattca acttttatgc tctcttctct tcgatctcgc ctttggctta
      901 gctacacctt tctcatcgtc acagctttga gtgttgtgac gattgttcta tttgcctcgc
      961 tgtttcgaaa tccgctttta tatcggcaga cgacagagcg cttgaacgca gtgcgaagtg
     1021 taatggtgac gcgtttaagc gaagcggatg caccgccaat ttctacattg gcaatgcgta
     1081 acgcgcagac gtttaatgtc cgtgtggctg tgttttccca gaaccgtgaa cttgtgtttg
     1141 ataccagcac agaagcagag cttattttcc caaccgttaa gcggttaacg cgtgccttgc
     1201 cattgacccg tgataccaat ggggcggcat ggttgtattc cgttgaaaag ctacccgatg
     1261 ggacgtttct catggtggca acgccccgtc cacgtaactc tgttcttaat atctttagtg
     1321 atgaatttat ccccgttatt gttttgggcg gtgggagcgc tttactgctc tcgctgattc
     1381 tggcgttcct cgtctctggg tggatcgcca gcccgctgca gaaagtcgtc aacgcggcgc
     1441 ggggaatgcc ttcagcggag gtaaagccgc ttgaagcaac gggcccgcac gaggtccagg
     1501 aattgacgcg ggcatttaat tccatgatgg cgcgtgtgct ggcgagtcaa cggtcgcagc
     1561 gtgagttcgt ggcgaacgtc tcgcatgaaa tgaagacccc actcacatcc attcaaggat
     1621 tcgcgcaagc cttgctcgat ggcaccgcga acacaaaaga gtcacagcaa aaggcggcgc
     1681 aggtgattta cagcgaatct gaacggatgc
//
LOCUS       DAOREF010000745         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62161_length_1704_cov_3.191025, whole genome shotgun sequence.
ACCESSION   DAOREF010000745 DAOREF010000000
VERSION     DAOREF010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62161_length_1704_cov_3.191025"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKI33_13775"
     CDS             <1..530
                     /locus_tag="PKI33_13775"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNM38133.1"
                     /translation="GKCIEPTLRLNPFYGRWIYRNYPVTYVSWFQAADYCAWAGRRLP
                     TEAEWEKAARGTDSRKFPWGNERPNPRLANYADSLIGEPLPVDRYPLGASPYGALNMV
                     GNVREWIADWYDANYYMNTPLNNPSGPETGFERSLRSGAYDADANEILTTSRYKHEPQ
                     SAGLSRGFRCVERDK"
     gene            complement(573..>1704)
                     /locus_tag="PKI33_13780"
     CDS             complement(573..>1704)
                     /locus_tag="PKI33_13780"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNM38134.1"
                     /translation="YIEAGGQVNDTGFIRGTDWEIEITGIRRASAGVIVHIGEVISGH
                     PKVGDKATAEVDATRRHDIMRNHTATHLLHKALHEILSDQAKQAGSLVSPTHLRFDFN
                     HPEAMTSDQIERVEKMVNEAVAMDMPVVKVTKSLDEAKKEGAMALFGEKYGEVVRTIS
                     IMEDEKDKYSYELCGGTHLERTSDVGAFIIVSESSAAAGVRRIEAVTGRGAYELIQKR
                     FKALKQTAGSLKTSVDEVPARVDALQDEVSELKKELANLRAQSALSTFNSLLANVQTA
                     KDVNVLGAEIPNADVDTLRSLADKFREKYPKNGAALFVSGNTVIATVTEDVVKRGVKA
                     GDLITGIGGKGGGRPNLAQGSLAGDVKEALMKLEKIMEEKVK"
BASE COUNT          359 a          479 c          461 g          405 t
ORIGIN      
        1 ctgggaaatg tatcgagccg acactgcgcc taaatccttt ttatggcagg tggatctatc
       61 gcaattaccc cgttacctat gtctcctggt ttcaagccgc agactattgc gcatgggcgg
      121 gcaggcgctt acccaccgaa gcggaatggg aaaaagccgc gcgcggcaca gacagccgaa
      181 aatttccatg ggggaacgaa cgaccaaacc cgcgcctggc caattatgcc gactcgctca
      241 tcggcgaacc cttgcctgtg gatcgctatc cattgggcgc aagtccgtat ggggccttga
      301 acatggtggg caacgtccgc gaatggatcg cagattggta tgacgccaat tactacatga
      361 acaccccgtt gaataacccc agcggtccgg aaacgggctt cgaacgttcc ctgcgcagcg
      421 gagcctacga tgccgacgcg aacgagatcc tcaccacctc gcgttacaaa cacgagccgc
      481 aatctgccgg gctgagtcgc ggctttcgct gcgttgagcg tgataaatag atcataaata
      541 aaacaggcga ctacatgtcg cctgttttat ttttatttca ctttttcttc cataattttt
      601 tcaagtttca tcagcgcttc tttgacgtca ccagcgaggc tgccctgtgc gagattggga
      661 cgcccgcctc ctttaccgcc gatgcctgtg atcagatctc cagccttgac tccccgtttg
      721 accacgtctt cggtcacggt ggcgatgacg gtgttacccg agacgaagag cgccgcgcca
      781 ttcttggggt atttctcgcg gaacttatcc gccagcgagc ggagcgtgtc cacatccgca
      841 ttcggaattt ccgcgccgag aacgttgaca tcctttgcgg tttggacgtt agcgagcaag
      901 gaattgaacg tagaaagcgc agactgagcg cgtaggttgg cgagttcctt tttgagttca
      961 gaaacttcat cctgcaaggc atccactctg gcagggacct catcgacaga agtcttgagg
     1021 gagcccgcgg tctgcttgag ggccttgaag cgtttctgaa tcaattcata tgcaccacgc
     1081 ccggtgaccg cttcgatacg acgcacacct gccgccgccg aactctcact gacgataatg
     1141 aatgcgccca catcagaagt gcgttcgagg tgagtaccac cgcacaactc gtaggagtat
     1201 ttatctttct catcttccat gatggaaatg gtgcggacga cttcgccgta tttctcacca
     1261 aagagcgcca tcgcgccttc cttctttgct tcgtcgaggg acttggtcac tttgaccacg
     1321 ggcatatcca tcgccacggc ttcgttgacc attttctcga cgcgttcgat ctggtcggag
     1381 gtcatggctt cgggatggtt gaagtcgaag cgcaggtggg tcggcgagac gagtgaacct
     1441 gcctgcttag cttggtcgct caaaatttca tgtagcgcct tgtgcagcaa gtgagtggcg
     1501 gtgtggttgc gcatgatgtc gtggcggcgg gttgcatcaa cttcggcggt ggctttgtca
     1561 cccactttgg gatgacctga gatgacttca ccaatatgca cgatcacgcc cgcagatgcg
     1621 cggcgaatgc cggtgatttc gatttcccag tccgtgccgc gaatgaagcc agtatcgttg
     1681 acctgaccac ccgcttcgat ataa
//
LOCUS       DAOREF010000746         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62234_length_1703_cov_3.818568, whole genome shotgun sequence.
ACCESSION   DAOREF010000746 DAOREF010000000
VERSION     DAOREF010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62234_length_1703_cov_3.818568"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKI33_13785"
     CDS             <1..196
                     /locus_tag="PKI33_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNM38135.1"
                     /translation="YRSMMGFGSGPHRCAGEHLALAETDVFIQKLLKLPGIRIESGPN
                     IIRNDTVEGYEVNDLIVAVD"
     gene            263..691
                     /locus_tag="PKI33_13790"
     CDS             263..691
                     /locus_tag="PKI33_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38136.1"
                     /translation="MKLNTKKEVWIARVIAWVLLCFISSGILFVTTFMVGPTLFVVPN
                     LTEKLAVAIYCPGAESTTLETGASTPTTSDPNGTTGHTVEITCTFPDGSTDIIDNGEY
                     AIASIGGMFGIGALIGLLISLPLFVVPFFLIRKRKEPSAL"
     gene            complement(736..1671)
                     /locus_tag="PKI33_13795"
     CDS             complement(736..1671)
                     /locus_tag="PKI33_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNM38137.1"
                     /translation="MSDLKRLMPFMVSILVVLACAIPSIPIQNVDTMNTNVANTVIAG
                     LTQSVTATFPASSTPSETLAYTSTPTLTYLTARVPSQTPTFTPLPGTDTAIPLLTDTA
                     EPSLVKISVTRPTHCRSGPGKSYEIVGSLLVGMKADVIGRDPTSEYWYIPNPYVFTDY
                     CWVWGEYAEFEGNQLSVPVVMPPPTPTSTATTIPTVDFKLLGSGHHSCDKTHWMNITI
                     LNESNFTFSSINIVMQDKVKNISRSVTYNNFPIQTGCEGLTWVETVPADASATVLGPK
                     FDYNIRGNKLHVTVTVCSEKDMKGACSTRETNLVP"
BASE COUNT          482 a          394 c          372 g          455 t
ORIGIN      
        1 ctaccgctcc atgatgggct tcggttctgg tcctcaccgt tgtgcgggtg aacatctcgc
       61 cttagccgaa accgatgtct tcatccaaaa gttgctcaaa cttccgggca tccgcatcga
      121 aagcggaccg aacatcatcc gcaacgacac agtagaagga tacgaagtca acgacttgat
      181 cgtagcagtc gactagaaaa aagacagcct ttgaaagagg ctgtcttttt atataaatga
      241 tgatttccat gagaggtgaa acatgaaact taacacaaaa aaagaggtgt ggattgcccg
      301 tgtgatcgct tgggtcttgt tgtgttttat ttccagtggg attttgttcg ttacaacttt
      361 catggtaggt cccactttat tcgtcgttcc aaacctcact gagaaacttg ccgttgccat
      421 atattgtccc ggtgcagaaa gcaccacgct tgaaacaggt gcatctacgc cgaccacttc
      481 agaccccaac gggaccacag gtcacaccgt ggaaatcacc tgcaccttcc cagatggctc
      541 caccgacatc atcgacaatg gcgaatacgc aattgcctcg atcggcggca tgttcggtat
      601 tggggcattg atcggacttt tgatttcatt acctttgttt gtagtaccgt ttttcttaat
      661 tcgcaaaaga aaagagccat cagctttata gcatctggct ctaaacacat aaggcacttc
      721 atacttcttt gttaactaag gcaccaaatt ggtctcacgg gtactacatg cacctttcat
      781 atccttctct gaacagacag taactgtcac atgcagttta ttaccacgga tgttgtaatc
      841 aaatttagga ccaagcacag ttgccgaagc atctgcagga accgtctcca cccaagtaag
      901 cccttcacaa cctgtctgaa tagggaagtt gttgtacgtc acagagcgag aaatattctt
      961 gaccttatct tgcatcacaa tattaatgga gctaaacgta aaattactct cattaaggat
     1021 cgtaatattc atccaatgtg ttttatcaca agagtgatgc ccgctgccga gtaacttaaa
     1081 gtcaacagtt ggaatagtcg tcgccgtact agtgggagta ggcggcggca taacaacagg
     1141 gacagataat tgattccctt caaattcagc atactccccc catacccaac aataatccgt
     1201 gaatacatag ggattaggaa tataccaata ttcagaagtc gggtctcgcc caattacatc
     1261 tgctttcatt cctaccaaaa gtgagccaac aatctcatat gattttcccg gaccggaccg
     1321 acagtgagtt gggcgagtaa cgctgatctt taccagactc ggttctgctg tatcggttaa
     1381 taatgggatt gccgtatccg taccaggaag tggagtaaag gtcggtgttt gagaaggcac
     1441 acgtgctgta agataagtca acgttggagt ggaagtgtat gccagggttt cagaaggtgt
     1501 actactggct gggaatgtag cggttacact ttgcgtcaac ccggcaatga cggtgttagc
     1561 aacattggta ttcatggtat caacgttttg tatgggaata ctaggaatgg cacacgccag
     1621 aacaactaga attgaaacca taaatggcat aagtcttttt aaatcagaca tagatgtctc
     1681 ctattacatc cccaattatg aca
//
LOCUS       DAOREF010000747         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62243_length_1703_cov_3.166262, whole genome shotgun sequence.
ACCESSION   DAOREF010000747 DAOREF010000000
VERSION     DAOREF010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62243_length_1703_cov_3.166262"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKI33_13800"
     CDS             complement(<1..329)
                     /locus_tag="PKI33_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38138.1"
                     /translation="MNLYLRLAWRNIWRHKRRTVIIVLAMSMSLSLMMFYDGLMNGFT
                     DAIYGNAVKVLGGNIQIHAEGYRAEVGSTPLLPLTDAQAVVNAAEADTNVVAATQRIN
                     TGGLVTNR"
     gene            complement(368..>1703)
                     /locus_tag="PKI33_13805"
     CDS             complement(368..>1703)
                     /locus_tag="PKI33_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNM38139.1"
                     /translation="FVGGVVVILLTVLAILTGNPMFIIPGGLFFLLGLVLIAPAMVRP
                     FALVFGRVIALFAARQGVGELAQGNITRQPSRVAVTASTSMLALAIIVAIGGMISSLT
                     GVLYELMEDGFGSDYIFVPPSVALWGSNVGAKPELAEQLKAVNGVEDVSTLRFASTTS
                     DGQAISILGIDPASFPAVSGLYFQDSLTSDADTYTSLASGRTIIINGAMSVALGKSVG
                     DEIALVTPNGTLTYQIIAIATDILNAKVVTGFISQANLQNDFGVTDDVYLQLNLKDDA
                     DRAAADATIRKLAENYPTFKVVSGSEYFGTLKSQMDAAFSGLYIVFLLLAVPSLIAML
                     NTLTISVIERTREIGMIRAAGATRRQVRTMVVAEALLLAAIGTAFGILGGLYLGYVLV
                     VSMEGIFPLGYAFPLSGILFAIAIGLLFGVFAAFIPARQASGMNVVEALRYE"
BASE COUNT          472 a          471 c          418 g          342 t
ORIGIN      
        1 cgattcgtca ccaaaccacc ggtattgatg cgttgagtcg cggcaaccac gtttgtatct
       61 gcttcggcgg cgttgaccac cgcctgtgca tcggtcaacg gcagcagcgg ggtggagccc
      121 acttctgccc gatacccctc cgcgtggatc tgaatgttgc cgcccaagac cttgaccgca
      181 ttgccataga tcgcatcggt gaagccattc atcaatccat catagaacat catcaacgac
      241 aaagacatcg acatggcaag tacgatgatg actgtgcggc gtttgtgccg ccaaatgttg
      301 cgccaggcaa ggcgcaggta gagattcata gataaactcc tttcgagtga acgttcaaaa
      361 ttattcttta ctcatatcgc agcgcctcga ccacattcat gccagatgct tgtcgggcag
      421 ggatgaatgc ggcgaacacg ccaaataaca aaccgatcgc aatggcaaag agaataccgc
      481 tcaaggggaa ggcatacccc agtgggaaga ttccttccat tgaaacgaca aggacatagc
      541 caagatacaa accacctaaa attccaaaag cggtgccaat ggctgccaaa agcaaagctt
      601 cggcaaccac catggtacga acttgtctgc gggtcgcccc agccgcgcgg atcatgccaa
      661 tctcacgcgt gcgttcgatg acgctaatgg tcaaggtatt gagcatggcg atcaacgacg
      721 gaactgccag caaaaggaaa acaatataca agccgctgaa tgccgcatcc atttgagatt
      781 tcaaggttcc gaaatattca ctacctgaaa ccactttaaa ggtgggatag ttctctgcca
      841 gcttgcgaat ggtggcatcg gctgcggcgc gatctgcatc gtctttcaag ttgagctgta
      901 aataaacatc atccgtaacg ccaaaatcat tttgcaaatt cgcctgagag ataaaccccg
      961 tcaccacttt tgcattgagt atgtcagttg caatcgcaat gatctgatac gtcagcgtgc
     1021 catttggcgt cacaagtgca atctcatcac caacagattt acccaatgca acactcatcg
     1081 cgccgtttat gatgatggta cgtccgcttg caagggaggt gtaggtatcg gcatcagagg
     1141 tgagcgagtc ttgaaagtag agaccagaca cggcggggaa agaggcgggg tcgatcccta
     1201 aaatggagat cgcctgtccg tccgatgtcg tgcttgcaaa tcgaagcgtg ctaacgtcct
     1261 cgaccccatt cactgctttc aattgctcag caagttccgg tttggcaccc acgttactgc
     1321 cccacaacgc aactgaaggc ggcacaaaaa tgtaatcact gccaaaacca tcttccatca
     1381 gctcatacaa gacgccggtc aaacttgaga tcatgccgcc aattgcaaca atgatcgcca
     1441 acgccaacat gctggtgctg gcagtcactg ccacgcgcga aggctgacga gtgatgttcc
     1501 cttgggcaag ctcacccaca ccctgacgtg ccgcaaagag cgcgatgaca cgcccgaaca
     1561 ccaacgcgaa aggacgcacc attgccggag cgatcaacac caaacccaat aggaagaaaa
     1621 gcccgccagg gatgatgaac atggggttgc ctgtgagaat ggcaagcacc gtaagcagga
     1681 tgacgaccac gcctccaacg aag
//
LOCUS       DAOREF010000748         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62270_length_1703_cov_2.432646, whole genome shotgun sequence.
ACCESSION   DAOREF010000748 DAOREF010000000
VERSION     DAOREF010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62270_length_1703_cov_2.432646"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKI33_13810"
     CDS             <1..338
                     /locus_tag="PKI33_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38140.1"
                     /translation="AKPYFQYLMKSFQDRNEFPLFERYWRMVSSFEQDREANVDVFSY
                     THADYGPLNYATATTVSITPHGELFLNQYLSLDDHTRNLFSTLEKQTGQGVVTELASW
                     PEKKIPKNA"
     gene            438..929
                     /locus_tag="PKI33_13815"
     CDS             438..929
                     /locus_tag="PKI33_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNM38141.1"
                     /translation="MTKLDSMVQNFLAQKRIAVVGVSDKRETGCNAAYQKFKDAGYEV
                     FAVNPRIASFQGDACYPDLAALPTKPDAVFILANPKVTDQIVSQCVDLGVKHVWMHCM
                     MGTKPGLATSTTSVSPTAIDVCKANGISVIPGSCPNQYLNPDGGHKFMNGMFKLFGFM
                     SVN"
     gene            1005..>1703
                     /locus_tag="PKI33_13820"
     CDS             1005..>1703
                     /locus_tag="PKI33_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38142.1"
                     /translation="MTPNSASPLIEIKNLVKTYKTPAGDFTAVKGIDVEVQRGEFVAV
                     IGKSGSGKSTFINMITGIDRPTSGEIYIDGAPVHSFSEAQMAAWRGRNLGIVFQFFQL
                     FPTLTLLENVILPMELNHLYTQKERRERAMDLLKLVEMDHQAHKLPTTISGGQQQRVA
                     IARSLANDPPLLIADEPTGNLDSKTAAKIFSLFEELVASGKTILMVTHDSDLARRVNR
                     TILISDGEVVNEYLV"
BASE COUNT          461 a          485 c          351 g          406 t
ORIGIN      
        1 gtgccaaacc ttattttcaa tatctcatga agtcattcca agaccgaaat gaattcccgc
       61 tcttcgaacg ctattggcgc atggtttcat cctttgaaca agaccgcgaa gcgaatgtag
      121 atgtgttcag ttacacccat gccgattacg gtccgctcaa ttatgctacg gcaaccaccg
      181 tttcaatcac cccacatggc gaactattcc tcaaccaata tctgtcgctc gatgaccaca
      241 cccgtaatct tttctccaca cttgagaaac aaacaggtca gggtgttgtc acagagcttg
      301 cctcatggcc cgaaaagaaa atccctaaga acgcctgata ttcgtcatat tgaaagcagg
      361 aatgtcctcc ttataatcat taatagcgcc acgcttctgt atcaagtagc gtaagcaata
      421 taaataagga gaatgtcatg acgaaactcg atagtatggt tcagaatttc ctcgcccaaa
      481 aacggatcgc cgttgtgggc gtttcagaca agcgcgaaac cggatgcaat gccgcctatc
      541 agaaattcaa ggatgcaggc tatgaagtct tcgccgtcaa cccgcgtatc gcctcattcc
      601 aaggcgacgc ctgttacccc gacctagccg ctctgcccac caagcccgat gcggtcttca
      661 tcctcgccaa ccccaaagtg accgaccaga tcgtcagtca atgcgtggac ttgggcgtga
      721 aacatgtgtg gatgcactgc atgatgggca ccaagcccgg tctcgccacc agcacaacca
      781 gtgtatcccc caccgcaata gatgtatgca aagccaatgg catctccgtc atcccaggct
      841 cttgccctaa tcaatacctc aaccccgatg gcgggcataa attcatgaat ggaatgttca
      901 agctatttgg ttttatgagc gtgaactgac tgcccaccaa ctttcttgaa cagtcatggc
      961 aaaaccatgg ctgttttttt attaattcga ttaaacttga caacatgact ccaaattccg
     1021 cctccccatt gatcgagatc aaaaacttag tgaaaaccta taagaccccc gctggagatt
     1081 tcactgccgt caaaggtatt gacgtggaag tgcaacgggg agagttcgtt gccgtcattg
     1141 gcaaatcggg cagcggcaaa tctactttca ttaatatgat caccggcata gaccgcccca
     1201 cctcgggcga aatctatatc gatggcgcac cagtccactc ttttagtgag gcgcaaatgg
     1261 cagcctggcg cggacggaat cttgggatcg tctttcaatt ttttcaactt ttccccacat
     1321 taaccctgtt agagaacgtc atcctcccaa tggagttgaa ccatctctat acccaaaaag
     1381 aacgccgtga acgcgccatg gacctgctca aactggtcga aatggaccat caggctcaca
     1441 aacttcccac cactatttca ggcgggcagc aacaacgtgt ggcaatcgcc cgctcccttg
     1501 ccaatgaccc accactgctc attgcagacg aacccaccgg caacctcgac tcaaaaactg
     1561 ccgccaagat attttcttta ttcgaagaac tcgtcgcttc cggtaaaacc attctgatgg
     1621 tcacacacga tagtgacctt gctcgccgcg tcaaccgcac cattcttatc tcagatggtg
     1681 aagttgtaaa cgaatatctt gtg
//
LOCUS       DAOREF010000749         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62366_length_1702_cov_2.071038, whole genome shotgun sequence.
ACCESSION   DAOREF010000749 DAOREF010000000
VERSION     DAOREF010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62366_length_1702_cov_2.071038"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..548
                     /locus_tag="PKI33_13825"
     CDS             87..548
                     /locus_tag="PKI33_13825"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM38143.1"
                     /translation="MDFKIEGPYLEKANLCAPIIRALPEWFGIEAAIIHYSTEIDILP
                     TFLAFEGKHVVGFLSIKQHNPYSAEVYIMAVLPESHHKGIGRLLIKHSQDWLKSKGVQ
                     YLQVKTLGSSHPDKSYAATRLFYEVVGFVPLEESTQIWDEHNPCLIMVKKL"
     gene            751..1332
                     /locus_tag="PKI33_13830"
     CDS             751..1332
                     /locus_tag="PKI33_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38144.1"
                     /translation="MIFAISPKHTTEDFKNGWNLDDKIDVFIARVEGWQLGVANTLVE
                     KNIPHRKFAILMIIINYFEMIAKYRDGYVEAIKGRRKGEGKSAHFFKEGLKDVFPDMS
                     LPEDELVTAAMYENVRNGLYHAGGTRNNVLLSDEIPNTVGYNTITGAIAINPDLLVRE
                     LQSHFARFSAKLRDPANTTLRANFEARFDVDNE"
BASE COUNT          519 a          376 c          360 g          447 t
ORIGIN      
        1 ccgtccccgc cccagcgcag gtaacgcaaa ccgttgggca gcaatagatt gactcaaaaa
       61 tagaaaagca aagggacgca attattgtgg atttcaaaat cgaaggtccg tacttagaaa
      121 aagcgaattt atgtgcgcca ataatacgtg ctctgcccga atggtttggt atcgaagcgg
      181 ctataatcca ttactcaact gaaattgata ttctcccaac atttctggct tttgaaggaa
      241 aacatgttgt tggcttcctc agcataaaac aacataaccc atattcagcc gaagtatata
      301 taatggcggt tcttccagaa tcacatcaca agggaattgg cagattatta atcaaacatt
      361 ctcaagattg gctgaaaagc aaaggcgttc agtatctaca ggttaagacc cttggttcat
      421 cacacccaga caaaagttat gccgctacca gattatttta tgaggttgtt ggatttgtgc
      481 ctttggaaga aagcactcaa atttgggatg aacataatcc ctgtttaata atggtcaaaa
      541 aactttgatt ggcgttgtcc cacttctacc aaaatgctgc ccaacaaagc gtgcacctga
      601 cgtgtgggag tctgcggcat tttcgagcat ttttctcgct tcgggttttt tctgctccca
      661 aacagaatcc acgcccgccc acacgcaggt aacgcaaacc gttgggcggc tctcgaaggt
      721 ttgagtcagg cattcatgaa gaagtaaact atgattttcg caattagtcc taaacacacc
      781 accgaagatt tcaaaaacgg ctggaatcta gatgataaga ttgatgtatt catcgcacgg
      841 gttgaaggtt ggcaactagg tgtggcgaat actttggtcg agaagaacat tcctcaccga
      901 aaatttgcta tccttatgat tatcattaac tattttgaaa tgattgctaa gtaccgcgat
      961 ggctatgttg aagcaatcaa gggtcgacgc aaaggtgagg gcaaatctgc ccacttcttc
     1021 aaagaaggtc tgaaagatgt ttttccagat atgtctcttc cagaggacga gcttgtaact
     1081 gcggcaatgt atgagaatgt ccgcaatgga ctttatcatg ctggtggcac aagaaacaat
     1141 gtattactct cagatgaaat tcccaacact gtcggttaca acactattac aggcgcaatt
     1201 gcgattaatc ccgatctgct ggtccgagaa ttgcagagcc atttcgcccg attttctgca
     1261 aaattaagag atcctgccaa tacaactttg agagcaaatt ttgaagccag gtttgatgta
     1321 gataatgaat aaaggtggca gaagccaaat acagaaagcc gcccaacacc gcgtgcacct
     1381 gacgctgggg agtctgcgca catcccaagc agtttcacac gccttagcct ttttatgtgg
     1441 ctggacggct tcgccgtccc cgccccagcg caggtaacgc aaaccgttag gcggttcctc
     1501 gcaagaaatt aagcttcgag caacaaacat aaattagatg aacacatcag atcaaatcaa
     1561 tatatttaca tcagtcattg cacttgctgc atttttgttg tctctttaca atttctatat
     1621 tgaccgccga gataagtcaa ccacggtaat tattcggcta tctcatggag atataaaaaa
     1681 aggcaaagaa aaaataacat aa
//
LOCUS       DAOREF010000750         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62449_length_1700_cov_4.143465, whole genome shotgun sequence.
ACCESSION   DAOREF010000750 DAOREF010000000
VERSION     DAOREF010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62449_length_1700_cov_4.143465"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..549)
                     /locus_tag="PKI33_13835"
     CDS             complement(148..549)
                     /locus_tag="PKI33_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38145.1"
                     /translation="MEFEEYINEVTRKTIQEGGSASKPSSCIFLPIQDLWCFPKYPAK
                     TSIFPPHINLAEELKNFIEKNITFLDEKDCWLGTWVNPQTGEYYLDISTGIENLEAAR
                     SFAIQAGQKDGREIVAMFNPKQRHTVFLKSV"
     gene            complement(576..1463)
                     /locus_tag="PKI33_13840"
     CDS             complement(576..1463)
                     /locus_tag="PKI33_13840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM38146.1"
                     /translation="MENFDQLKHQTSGKPKYKLAHANVFVSSGGFHYIDHLDPMEEIS
                     PEIDGSNLTEAEITYIEKSLQSNPERLENQIAAVKRHTIITGKEALDIGCGGGLFLSK
                     LKAEGANVTGVELSDTRAYYSKTKYGFEVIKRTIEDEYWNAFQGRFDIVTLWDVIEHV
                     NYPLATLQAAAKLLKPGGILLIDTPCRDAFYHRFGEFTYKLSGGKFPTFLNTMYSAKP
                     FGHKQILSLAEMRGLYEAVGMEVVELKRFHELSFPYSFYLKKLVRSDFLVKVLLPFVY
                     IFFAIFPIRNKMLAIGKKA"
     gene            complement(1450..>1700)
                     /locus_tag="PKI33_13845"
     CDS             complement(1450..>1700)
                     /locus_tag="PKI33_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNM38147.1"
                     /translation="SPDLIVHLYADPETIRQRLALRNRINIASSEDAELLESYVTEWL
                     ETVPETNILRLDVSKESLDYPISVPRILDKIRGTHGKF"
BASE COUNT          464 a          346 c          342 g          548 t
ORIGIN      
        1 gttgaataat cttgcccctg tatttgaccc attactccgt tctgttgcct tgttgtttgc
       61 cctgagtgat atcgtacata ttgtgctgct tattccctta tatggaattc ggcaagtgat
      121 gacatataca atgcaacttc aagtaaacta aactgatttg agaaagactg tatgtcgctg
      181 tttaggatta aacatggcaa cgatttctct cccatctttt tgacctgcct gtatggcaaa
      241 tgaccgtgca gcttcaaggt tttcaattcc agttgaaata tccaaataat attcaccagt
      301 ttgcgggttg acccaagtcc caagccagca atctttttca tcgagaaatg ttatattttt
      361 ctcgatgaag ttttttaact cttctgctaa atttatatga ggcgggaaaa tagaagtctt
      421 tgcgggatat ttaggaaaac accacaaatc ttgaattggt aaaaatatac aggatgacgg
      481 tttcgacgcg ctgccgcctt cctgtatcgt ctttcttgta acttcgttta tgtattcctc
      541 gaactccata tatagcagct atgttttaaa gaaatctacg cctttttccc aattgcaagc
      601 attttgttgc gaataggaaa tatcgcaaaa aagatatata caaatggaag tagaaccttt
      661 actaaaaagt cagaacgtac aagctttttt aggtaaaaac tatatgggaa agaaagttcg
      721 tgaaagcgtt ttagctctac tacttccatg ccaactgctt cgtacaatcc gcgcatttct
      781 gcgagcgata gaatttgctt atgcccgaat ggctttgctg aatacatggt atttaagaat
      841 gtcgggaatt tccccccaga gagcttgtat gtaaactcgc caaaccgatg atagaaagca
      901 tcgcggcatg gtgtgtcgat caataaaata ccgccgggtt ttagtaattt tgctgcagct
      961 tgtaaagttg ccaatggata gtttacgtgt tcgatcacat cccacaaagt gacaatatca
     1021 aaccgaccct gaaaagcatt ccaatactca tcctcaatgg tacgcttgat tacttcaaag
     1081 ccatatttgg tttttgagta atatgcgcga gtatcgctta actctacgcc cgtcacattt
     1141 gcaccttccg ctttaagttt tgaaagaaat agcccgccac cacaaccaat atccaacgcc
     1201 tctttgccgg taattatcgt atgtcgtttt acagcggcaa tttgattttc aagccgttcg
     1261 gggttcgatt gtaatgattt ttcgatgtat gtaatttcag cttcggttag attgctgcca
     1321 tcgatttctg gtgaaatttc ttccattggg tcgagatggt cgatatagtg aaatccacct
     1381 gacgaaacaa atacattcgc atgagctaat ttgtattttg gcttaccgct tgtttggtgt
     1441 ttgagttggt caaaattttc catgagtacc cctaatttta tcaagaatgc gcggtacgct
     1501 gatggggtaa tctaagcttt ctttggaaac atccagtcgg aggatatttg tctccggaac
     1561 cgtttctaac cattcagtga cataagactc taagagttcc gcatcttcgc tggatgcgat
     1621 attgattctg tttctcaagg ctagtctttg gcggatagtt tctggatctg cataaagatg
     1681 gacgatgaga tcaggcgatg
//
LOCUS       DAOREF010000751         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62531_length_1699_cov_3.621046, whole genome shotgun sequence.
ACCESSION   DAOREF010000751 DAOREF010000000
VERSION     DAOREF010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62531_length_1699_cov_3.621046"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKI33_13850"
     CDS             <1..248
                     /locus_tag="PKI33_13850"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38148.1"
                     /translation="LIDISIDKKTGRLLHEPEIITRGFVSPEDAETLLPDVRKLVLRM
                     VHDDGFDDEKDIINAVKSYLHQQTKRRPMVFVTLSKS"
     gene            complement(274..1311)
                     /gene="mutY"
                     /locus_tag="PKI33_13855"
     CDS             complement(274..1311)
                     /gene="mutY"
                     /locus_tag="PKI33_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNM38149.1"
                     /translation="MKNRLATQLLNWYHAQKRILPWRDHPDPYAVWVSEIMLQQTRVD
                     TVIPYFEKWMKLFPTVTALASASEQEVLNAWEGLGYYSRARNLHKAAKVVVEKFNGEL
                     PRDLEDLRSLPGIGRYTVGAIASMAFGMDEPTLDGNLRRVFSRLFDVDEFADLPVGEK
                     ILWELAAQNLPEGEAGDYNQALMDLGATICLPKNPRCLLCPLMDLCKARENGTQELRP
                     VLKPKKAVPSYVHVAAVIIERGRVLLSQRPAEGLLGGMWEFPNARIEGDPAKALVKGL
                     KAATQIQVKKKEALQVVEHAYSHFKVTVYPFVCEKVSIPKNKNLKWVKLTELEEYPMG
                     KVDRQIARQIK"
     gene            complement(1311..>1699)
                     /locus_tag="PKI33_13860"
     CDS             complement(1311..>1699)
                     /locus_tag="PKI33_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38150.1"
                     /translation="TSTQDELVAKLVTAGYDQNEIDIFLQQPKAILNEGRLLFPRMYW
                     RGEGLASANPWPAYAVQDFPRIGFILINANSQNLIYPTKEVLDFPQGADSIVLACAGD
                     NDLLYVRVIAFDDATYQNTPLTEPCP"
BASE COUNT          415 a          474 c          389 g          421 t
ORIGIN      
        1 tgctcatcga tatcagcatc gacaagaaaa ccggtcgcct cctacatgag cctgagatca
       61 tcacccgcgg cttcgtctcg ccagaagatg ccgaaaccct cctgcccgat gtccgcaagc
      121 ttgtcctgcg catggtccat gatgatggct tcgacgatga aaaagatatc atcaacgccg
      181 tgaagagcta tctacaccaa caaaccaaac gcagaccgat ggtcttcgtt acactaagca
      241 aatcctaaaa caaaaatgac cgtcgcaagg cggtcatttt atttgccggg caatctgccg
      301 atctaccttc cccatcgggt attcttccaa ctcggtcagc ttcacccact tcaaattttt
      361 atttttgggg atggaaacct tctcgcacac aaacggatag actgtgactt taaagtggct
      421 gtacgcatgt tcgacgactt gcaacgcctc ttttttcttg acctgtatct gggtcgctgc
      481 cttcaagcct ttcaccaacg cctttgccgg gtcgccctct attcttgcgt tcgggaattc
      541 ccacatgccc cccaacaatc cctccgccgg gcgttgactg agcaacacgc gcccgcgctc
      601 tataatcacc gccgccacat gaacatagga tggcactgcc ttcttcggct tcaatacagg
      661 gcgcaattcc tgcgttccgt tttcacgtgc tttgcataag tccatcaatg ggcaaagcaa
      721 acagcgcgga ttcttcggca ggcagatcgt cgcgccgagg tccatcaggg cttggttgta
      781 gtcgcctgct tcaccttcgg gcaaattctg cgccgccaac tcccagagaa ttttttcgcc
      841 gacaggtaaa tctgcaaatt catctacgtc aaataatcga gagaacaccc gtcgcaaatt
      901 accatcaagt gtgggttcgt ccatgccaaa tgccatggat gcaatcgccc caacagtata
      961 gcgaccaatc cccggcaggc tacgcaaatc ttctaaatca cgtggaagtt cgccgttgaa
     1021 tttctctaca accactttag cggctttgtg caaattccgc gcacggctgt aatagcccaa
     1081 gccttcccat gcattgagca cctcctgctc gctggcagaa gcaagcgctg ttacggtggg
     1141 aaataacttc atccattttt cgaaatatgg aatgactgtg tccacccgcg tttgttgaag
     1201 catgatctcg gaaacccaca ccgcgtatgg atcggggtgg tcgcgccagg ggagaatccg
     1261 cttttgggcg tggtaccagt tcaggagttg agtcgcgagg cggttcttca tcagggacag
     1321 ggttcggtta gtggggtgtt ttggtaagta gcatcatcaa aagcaatcac gcggacgtat
     1381 aacaaatcat tatccccagc acatgcaagg acaatgctat ctgcaccttg gggaaaatcc
     1441 aacacttctt tggttgggta aatcaaattc tgagaatttg cgttgatcaa aataaaccca
     1501 atacgcggaa aatcttgcac ggcatatgcg ggccacgggt tcgcagaagc aagcccttca
     1561 ccgcgccagt acatgcgcgg gaacagcaac ctaccttcat tgagaattgc tttgggttgt
     1621 tgcagaaata tatctatctc gttctggtca tatcctgcgg ttacaagctt tgccaccaac
     1681 tcatcttgag ttgatgtgt
//
LOCUS       DAOREF010000752         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62767_length_1696_cov_4.277879, whole genome shotgun sequence.
ACCESSION   DAOREF010000752 DAOREF010000000
VERSION     DAOREF010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62767_length_1696_cov_4.277879"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /gene="trpA"
                     /locus_tag="PKI33_13865"
     CDS             complement(<1..680)
                     /gene="trpA"
                     /locus_tag="PKI33_13865"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNM38151.1"
                     /translation="MTSRIEETFQKKPIFMPYFPLGYPDLETSVDVIEALAKNGADLI
                     EVGLSFSDPLADGPVIQQATQVALEKGITVKKSLEAVKELRKRGVTIPLILMGYYNPI
                     LAYGLEKFIRDAKEAGADGFIIPDLPLEEAEEFQSINGDMPLIQMLAPTSPDERMETI
                     ARNAKGFIYLVSVTGVTGERREVSNDLGDLINRVRSHTSAPVCVGFGIGTPEQAKQVG
                     QLADGVIVG"
     gene            complement(677..1345)
                     /locus_tag="PKI33_13870"
     CDS             complement(677..1345)
                     /locus_tag="PKI33_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38152.1"
                     /translation="MDTILQVFQAVGYLLLLVLLLVLWRKFVQRQEVRAFWFLLALAL
                     TINLLGNIAWIIHDMVTGRSLGTFSGIDLFYVSRYVLIGIALWLYPLSLPRKAGIWTG
                     IVTVIVTVLIWVVYFPPAMKLRDGDGWGFLGVAMYPALDTALIVLAWFRVLQNKEKGT
                     FFLLLLFSMVSYGIANTLNLTGYVFPSLNSGVLPNIFWILTDVLLLIMAFRDNEIKKI
                     RNEK"
     gene            complement(1418..>1696)
                     /locus_tag="PKI33_13875"
     CDS             complement(1418..>1696)
                     /locus_tag="PKI33_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38153.1"
                     /translation="GDQMTQMNGASKEIYEGDMVMKDEHGICCSIIYGQDNLSPISPE
                     TTHALYVVYTPVGVPVESVEAQLRAIEANIRLFTPSLVVEQSRVLTNS"
BASE COUNT          490 a          437 c          331 g          438 t
ORIGIN      
        1 ccgacaatga ctccatccgc gagttgaccg acctgtttgg cttgctcagg cgtgccgata
       61 ccaaacccca cacacaccgg cgcggacgtg tgacttcgca cacgatttat caaatcgcca
      121 aggtcatttg aaacttctct gcgttcaccc gtcacacctg tgacagatac caaataaata
      181 aacccctttg cattgcgggc aatcgtctcc atgcgctcgt cgggcgaagt tggcgcaagc
      241 atttgaatca atggcatatc gccattgatg gattgaaatt cctccgcttc ttccaatggt
      301 aaatctggga tgataaatcc gtccgcgcct gcttccttcg cgtcgcggat aaatttctcc
      361 aatccgtatg ccagtatggg gttgtaatat cccatcaaaa tcaacgggat ggtcacgcca
      421 cgcttgcgga gttccttcac cgcttccagc gatttcttga ccgtgattcc cttctccaaa
      481 gcaacttgcg tggcttgctg aatcacaggt ccatcggcga gcgggtcgga gaaagacaga
      541 ccaacttcaa tcaagtctgc accgtttttg gcgagggctt cgatcacatc tactgatgtt
      601 tccaagtcgg ggtatcccaa cggaaagtaa ggcataaaaa taggtttctt ctgaaacgtc
      661 tcttcaattc ggctcgtcat ttttcattcc ttattttttt tatttcgttg tctctaaatg
      721 ccataatcaa tagaagcaca tctgtcaaaa tccaaaatat atttggtaat acaccgctat
      781 tcaacgaagg gaaaacatat cctgtaaggt tgagcgtatt tgctattccg tacgacacca
      841 ttgagaaaag caaaagtagg aagaacgtac ctttttcttt gttttgtaga acacggaacc
      901 aagctaacac gatcaaagcc gtatcgagcg ccgggtacat cgccaccccc aagaatcccc
      961 acccatctcc atcgcgaagc ttcatggctg gcgggaaata cactacccag atcaatacag
     1021 tgacaattac cgtcacaata cctgtccaaa ttccagcttt gcgaggcaaa gataaaggat
     1081 ataaccacag tgcaatgcca atcaatacat aacgagatac ataaaacaga tcaattccag
     1141 agaatgtgcc taaagacctg ccggtcacca tgtcatgaat gatccatgca atgtttccaa
     1201 gcagattaat tgtgagtgct aaagcaagca aaaaccaaaa tgcgcggact tcttgtcgct
     1261 gtacaaactt tctccaaaga accagtaaaa gtactaacag cagtaaatat cctactgctt
     1321 gaaaaacctg caaaatcgta tccatttttt ctccgttgcg aatttacacc gtgcgcaaag
     1381 taaattctaa ccatttttcg ctgtacaaaa gccactttca agaattggtg agcacacggc
     1441 tttgttctac aacaagagaa ggtgtaaaca aacggatatt tgcttcaatc gctcgtaatt
     1501 gcgcttccac agattcaacg ggaacaccta caggtgtata cacgacatac aacgcatgag
     1561 tagtctctgg tgaaataggc gaaagattat cctgcccata gatgatgctg caacaaattc
     1621 catgctcatc cttcatcacc atgtcgcctt catatatctc tttcgaagcg ccgttcatct
     1681 gggtcatctg atcacc
//
LOCUS       DAOREF010000753         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62972_length_1693_cov_4.648352, whole genome shotgun sequence.
ACCESSION   DAOREF010000753 DAOREF010000000
VERSION     DAOREF010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62972_length_1693_cov_4.648352"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKI33_13880"
     CDS             <1..187
                     /locus_tag="PKI33_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38154.1"
                     /translation="LSMPGVVAGTILVFIPVMLQSGRLSVNTQDDNVASLRLYSKLGF
                     LRTGEHYPVFIHSLGAS"
     gene            187..795
                     /locus_tag="PKI33_13885"
     CDS             187..795
                     /locus_tag="PKI33_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38155.1"
                     /translation="MIPARLSPRLMNRFVETLSKEVGADTYHAVLSNSGLSRDWQKPE
                     FFLAFDNKQTAEAYAKLQSALRTYYGRGARGILLRIGSKLWEPMLNDASFASKAQATL
                     IRGFPKPLKRKPALELLASTLGAGRGDVTVHTLDLDLLLVDHSSPTTLGQKENSPVCF
                     VTLGLIRECLYWATGDEHDIEELVCKAAGSHQCEFKITIGAS"
     gene            798..1637
                     /locus_tag="PKI33_13890"
     CDS             798..1637
                     /locus_tag="PKI33_13890"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNM38156.1"
                     /translation="MKHQEFNWKAKDGLDIFAQVWEPDVVLPRAVVCLVHGLGEHSSR
                     YAHVAEAFSKEGYILFSFDHRGHGRSGGIRGHISSIEDFLQDIDQLLSQARTRYPNLP
                     VILYGHSLGGILVLNYSLNRKPDIKGVIATSSGLHTALEEQPVKIFMAKLLGSLMPKT
                     TLPSGLDAKAISRDETVVQTYNNDPLVHDKISLGFGKIMLGVTQWTLEHASEFPLPLL
                     LLHGKSDTIAYPSSSTEFAAALKDRATLVLWDGAYHELHNEPEKAEVFKTMLMWMDAR
                     LRG"
BASE COUNT          415 a          384 c          401 g          493 t
ORIGIN      
        1 gctttccatg ccgggcgtgg tggctggaac catcttggta ttcatcccgg tgatgcttca
       61 gtctggtaga ctatctgtca acacgcagga tgataatgtc gcgtcattgc gtttgtattc
      121 gaaacttggc tttcttcgca caggagagca ctaccctgtt tttatccatt ccttgggagc
      181 gtcatagtga tacctgcccg actttctcca cgcctaatga atcgcttcgt agaaactctt
      241 tcaaaagaag tgggcgccga tacctatcat gcagttttat caaattctgg tttgtcacga
      301 gattggcaga agccagaatt ttttcttgcc ttcgataata aacaaactgc agaagcatat
      361 gccaaattgc aatcagcttt gcgcacctat tatgggcgcg gcgcacgcgg tatccttttg
      421 agaatcggtt ctaagctttg ggaacccatg ttgaacgatg cttcttttgc ctctaaagct
      481 caggcaactc tcatacgcgg gtttcccaaa ccattgaagc gtaaacctgc gcttgaactc
      541 cttgcttcaa cccttggtgc gggacgcggt gatgtcacgg ttcatacttt ggatcttgac
      601 ctgcttctgg ttgaccattc ttctcctacc acgttgggac agaaagaaaa ttcccctgtg
      661 tgttttgtaa ccctgggctt gatccgtgaa tgtttatatt gggcaactgg cgatgaacat
      721 gacattgaag aacttgtctg taaagcggcg gggtctcatc aatgtgaatt taagatcacg
      781 attggagcat catagacatg aaacatcaag aatttaattg gaaagcaaaa gacgggcttg
      841 atattttcgc tcaggtttgg gagccagatg tggtgttacc ccgtgccgtg gtgtgcttag
      901 tgcacggctt gggtgaacat agttcacggt atgctcatgt tgccgaagca tttagcaaag
      961 aaggatacat tctcttctcc ttcgatcacc gcggacatgg tcgctctggc ggtatacgtg
     1021 ggcatatctc ttctattgaa gacttcctac aagatataga tcagttatta tctcaagcaa
     1081 ggactcgata cccgaacctg cctgttattc tgtatgggca tagcttaggt ggtatcctag
     1141 tcttgaacta cagtttgaat cgcaagccgg atattaaggg tgtcattgct accagttctg
     1201 ggcttcatac cgctttggaa gaacaacctg taaagatatt tatggcaaaa ttgcttggat
     1261 ctctgatgcc caagacaacc cttcctagtg gtttggatgc aaaagccatc tctcgtgatg
     1321 aaacagttgt gcaaacctac aataatgacc cgcttgttca tgacaaaatc tctctcgggt
     1381 ttgggaagat tatgcttggg gtcactcaat ggactttgga gcatgctagc gagttcccac
     1441 ttccgttgtt attgctacac ggtaagagcg atactattgc gtatccttcc agcagtactg
     1501 agtttgccgc tgctctgaaa gaccgtgcca cccttgttct ttgggatggt gcctatcatg
     1561 aacttcataa cgagccggaa aaggcagaag ttttcaagac catgctgatg tggatggacg
     1621 ctcgcctgcg aggctgatcg aaaataagaa cctgttaacc catttctgga caaatctatt
     1681 ttggcatgag aaa
//
LOCUS       DAOREF010000754         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_62977_length_1693_cov_4.081197, whole genome shotgun sequence.
ACCESSION   DAOREF010000754 DAOREF010000000
VERSION     DAOREF010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62977_length_1693_cov_4.081197"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="PKI33_13895"
     CDS             <1..1404
                     /locus_tag="PKI33_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM38157.1"
                     /translation="TGIAVIDDYGLVRNPPPDNEMYGVKQHITLAAWSGDTPVALISV
                     DNLLTQKPITPADIEALQLFAGYAGLAIENARLHTGLENLIEKRTDELTQSQKSLQLF
                     LDTANDLIQSLDESGRYIYVNQSWCRTLGFTLEEASKLDMLQVVDKEYQDHCLNIFRN
                     MMADGVSQMIEVGFRTKQGHLVIVEGSISIQTRSDGKRVTNGFFRDVTERKQAEIAMR
                     QANLEMEKALRMKDEFMANMSHELRTPLNAILGLSESLLEETVGSINSRQHKYLNTIS
                     ESGRHLLELINDILDLAKIESGQIHLASNSVNIKTLCEASLRIVDQLAKSKKQAMSLE
                     VADEIKFVQADERRLKQMVVNLLSNAIKFTPENGQVGIRALIDASRTFLNIEVWDTGI
                     GIKADDLSHIFQPFIQLNSGLARESSGTGLGLALVAQMARLHGGSITVRSEIGKGSSF
                     ILSLPWTPELPDAPQEQ"
     gene            1405..>1693
                     /locus_tag="PKI33_13900"
     CDS             1405..>1693
                     /locus_tag="PKI33_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM38158.1"
                     /translation="MAFSVILAPMLSEWIETNQIKLHVVTDGPKDGTPVILLHGFPEF
                     HYGWRKQVPALTEAGFYVITPDQRGYNLSDKPKGVSAYNLENLAKDVIGLFD"
BASE COUNT          497 a          416 c          394 g          386 t
ORIGIN      
        1 acaggcattg ccgtgatcga cgattacggg ctagtccgca acccaccacc agataatgag
       61 atgtatggag ttaagcagca catcactcta gccgcctggt ctggagatac gccagtggct
      121 ttgatctctg tcgataactt attgacccaa aagcctatta ccccggcgga tattgaagcc
      181 ttgcaattgt ttgcagggta tgccgggctt gccatcgaaa acgctcgcct acataccggg
      241 cttgaaaacc tgatagaaaa acgaaccgat gaattaacgc aaagccaaaa aagtctgcaa
      301 ctgtttttag acaccgccaa tgacctaatt caaagcctcg atgaaagcgg acgctatatc
      361 tatgtcaatc aatcctggtg tcgaacatta ggattcaccc ttgaagaagc cagcaaatta
      421 gatatgctcc aagtagtgga caaagaatat caagaccact gtttgaacat ttttagaaat
      481 atgatggcag atggggtgtc gcagatgatt gaggtcggct ttcgcaccaa gcaaggtcat
      541 cttgtgatcg tggaaggcag tatcagcatt caaacacgaa gcgacgggaa acgggtcacc
      601 aatggtttct tccgtgatgt gaccgaacgc aaacaagccg aaatcgccat gcggcaagcc
      661 aacctcgaaa tggaaaaagc actacgcatg aaagatgaat tcatggcaaa tatgagccat
      721 gaacttcgaa ccccgctcaa tgcgatttta ggactttcag aaagcctgct cgaagaaact
      781 gtcggctcca tcaattcccg gcaacataaa tatctcaaca ccattagcga aagcggacgt
      841 catctactgg aattgatcaa tgacattctg gatctggcaa agatcgaatc agggcaaatc
      901 cacttagcct ccaactctgt aaacatcaag accttgtgcg aagcctctct ccgcatcgtt
      961 gaccaactgg caaaaagcaa aaaacaagcc atgagtctcg aagtcgccga tgagattaag
     1021 tttgtccaag cagatgaacg ccgcctcaaa caaatggttg tcaacttgct tagcaatgcg
     1081 attaaattta cgcccgagaa cggtcaagta gggatacggg cattgatcga tgcgtcacgc
     1141 acctttttaa acatcgaagt ctgggatacg ggcatcggca tcaaagcaga tgacctctcg
     1201 cacatcttcc aacccttcat tcaattaaat tctggtttgg cgcgtgagtc atcgggcaca
     1261 gggttggggt tggcattggt ggcacaaatg gctcgcctcc acgggggcag tattacagtt
     1321 cgcagcgaaa ttggaaaagg cagcagtttc attctaagct tgccctggac gccagaactg
     1381 ccagatgccc ctcaggagca ataattggcg ttttccgtta tactcgcgcc aatgctaagc
     1441 gaatggatcg aaaccaatca aataaaactt catgtcgtga ccgacggacc caaagacggg
     1501 acgccggtca ttttattgca tggcttccct gagtttcatt atgggtggcg caagcaagtc
     1561 ccagcattga cggaggcggg cttttacgtc atcacgccag atcaacgcgg ctacaaccta
     1621 tcggataaac ccaaaggcgt ttctgcctat aaccttgaaa atttggcaaa agatgttatc
     1681 ggtctattcg acc
//
LOCUS       DAOREF010000755         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_63056_length_1692_cov_3.897373, whole genome shotgun sequence.
ACCESSION   DAOREF010000755 DAOREF010000000
VERSION     DAOREF010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63056_length_1692_cov_3.897373"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKI33_13905"
     CDS             <1..656
                     /locus_tag="PKI33_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38159.1"
                     /translation="FMVHPILVNMGELAYGIYILHIPVKWLFEWALTSAGITNVEVVM
                     YWAFLPLILSIAFIVHFYLDTPFRKWLKTFLKNISLRILTLDFILILLLAYFMFPARF
                     GDGREYRSYQEMERLVLWLAFFIRPAMALLFGTYRHEIVSKQGNQWLISITLSALIGS
                     LLIAVSVYIGYFTGWFENFPRSIFVIDWGIYTVFSILIRFAFRKLGVYKQEIEIMPL"
     gene            734..>1692
                     /locus_tag="PKI33_13910"
     CDS             734..>1692
                     /locus_tag="PKI33_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38160.1"
                     /translation="MKNLIRSADFLRPYVWQVLASLLMLLVLTGLNLVIPRIIQNVVD
                     DGLLRGDTIYLVRSALLLFGLGVVSALLGLGNRYASEWVAAHVGYDLRNRMYDHIQYL
                     PFAYHDHAQSGQLISRCIEDVRAIEKFAGSSIPELMRFVLLTLGILGVMLTTNSKLAI
                     ISLLPMIPLIVMTGWFGTKIGKLFFEVDQAVGEVSNRLQENVTGVQVVRAFAREDYEI
                     KRFESANMDVYHKWLHVINEWAKIMPTTNWLITIGTILILYFGGQMVIAGELTVGAVV
                     AFNAYLLMMAEPAQALTGLVNAGGEAAAGAQRVFEVLDTEPEI"
BASE COUNT          406 a          301 c          413 g          572 t
ORIGIN      
        1 aatttatggt tcaccctata ttagtaaata tgggtgaatt agcatatgga atttatattc
       61 ttcatatccc agtgaaatgg ctctttgaat gggcactgac ctcagcaggg attactaatg
      121 tagaagttgt tatgtattgg gcgttcctgc cattaatttt gagtattgct tttatagttc
      181 atttttattt agatacgcct ttcagaaaat ggttgaagac ctttttgaag aatataagtc
      241 taagaatatt aaccctggat tttattctga tcttattgct tgcatatttt atgttcccag
      301 ctcgctttgg cgatggtcgt gaatatcgtt cttaccaaga aatggaacgg ctggttcttt
      361 ggcttgcgtt ttttattcgt ccagcaatgg cattattatt tggtacatac cgacatgaaa
      421 ttgtttcgaa gcaaggaaac cagtggctaa tatctataac tcttagcgcg ttaattggct
      481 ctttattaat tgctgtttct gtatatatag ggtattttac gggttggttc gagaatttcc
      541 cccgtagtat ttttgtaata gactggggca tttatactgt attctctata cttatccgat
      601 ttgcttttcg caagttgggt gtgtataaac aagagattga aatcatgcca ttgtagagat
      661 ctaagatact taagcggaca ttctttgaaa agaatgtccg ctttttgttt atggtatgct
      721 taactccccg aatatgaaaa atctaatacg ctctgctgac tttcttcgac cttatgtctg
      781 gcaggtgttg gctagcctgc ttatgttgtt ggttcttacc ggattgaacc tggttattcc
      841 gcgtattatc caaaatgtag tagatgatgg acttttgcgt ggcgatacga tttatctggt
      901 ccggtctgcg cttctcttgt tcggacttgg agttgtttca gcgctgctag ggttgggcaa
      961 ccgttatgct tctgagtggg tggcagcgca tgttggttac gatctgcgca accgcatgta
     1021 tgaccatatt caatatttgc cctttgccta tcatgaccat gctcaatctg gacaattgat
     1081 ttcgcgttgt attgaagatg tgcgtgcgat tgaaaaattt gcagggtctt ccatccctga
     1141 attgatgcgt tttgttttgc ttacgctggg gattttaggg gtgatgctta cgacaaatag
     1201 caaattggcg ataatctctc tcttgcccat gattccgctg atcgtaatga caggctggtt
     1261 tggaacaaag atcgggaagc tgttttttga agtcgatcag gcggtaggag aggtctctaa
     1321 tcgcttgcag gaaaatgtga ccggggtaca ggtcgtccgt gcttttgcac gagaggatta
     1381 tgagatcaag cgttttgagt ctgccaacat ggatgtgtac cataaatggc tgcatgtgat
     1441 taatgagtgg gcaaaaatta tgccaacgac aaactggttg atcacaattg gaacgatttt
     1501 aatcctttac ttcggcgggc agatggtgat tgctggcgaa ttaaccgtcg gtgcggtggt
     1561 agcattcaat gcgtatttgc ttatgatggc agaacccgct caagcgttga ccggtttggt
     1621 taacgcaggc ggagaggctg cggcgggtgc tcaacgggta ttcgaagttt tggatacaga
     1681 accagagatc ag
//
LOCUS       DAOREF010000756         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_63275_length_1689_cov_4.242962, whole genome shotgun sequence.
ACCESSION   DAOREF010000756 DAOREF010000000
VERSION     DAOREF010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63275_length_1689_cov_4.242962"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PKI33_13915"
     CDS             <1..1018
                     /locus_tag="PKI33_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38161.1"
                     /translation="VVFAIDHAPEDGRYANALRRTLEKHGHRSIEPGETPQAVLVLIS
                     AFKKTTEYDLDTVAVYPILLQAVTDIDRKLQRIQWIDFRKGMGSADKLAKLLPEPQKL
                     LKVLAVAPTGSQEIFPLAVNALQYFYLIAGVFGGGGLLISTLSFASLIASGVFGIEYI
                     SQIFVAILNGAFLFLVAFSAVRGLRTRLGGSLALYPLLILMGFVYLINFANFSALFVD
                     SNSEETSQLAFTAVNGAGFALWFLPMGTFLIIPFLLFGWQGLYRWIPLRQTDNVTSLE
                     SIFLFYAPRKKGRLVFHILFHLLLAVIVIAAMQLDSGEILTDLLVVVMMAVFFRWLAR
                     RNLK"
     gene            complement(1104..>1689)
                     /locus_tag="PKI33_13920"
     CDS             complement(1104..>1689)
                     /locus_tag="PKI33_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38162.1"
                     /translation="PIAYLGDRECGSNSYNKVSSSIRKDIVMGLFGFGKKANKNAPVL
                     SADEVRTKILELNRETAPYQIIDGKDKGVDLIAEWKIVDAKWYELFAKANITKVFRIY
                     MKFDPAKNEVRAKDEEYTVEWRAGVPSLSISVSKFQGQMTSVEFGAAYAFTEELKPGL
                     VYNYRFNTNEIKKPIQEAVAACGWNYKGVAFGKL"
BASE COUNT          384 a          402 c          360 g          543 t
ORIGIN      
        1 ggtcgtcttt gcaattgacc atgctccaga agatgggcgt tatgccaacg ctttacgacg
       61 cacgcttgaa aaacatgggc accgttccat cgaaccggga gaaacccctc aggcagtgtt
      121 ggttctcatt tcggcgttta agaaaacaac agaatacgac ctcgatactg tggcggttta
      181 cccgattctc ttacaagcag tcacagatat tgatagaaaa cttcagcgca ttcaatggat
      241 cgacttccgc aaagggatgg gttcggcaga taaattggct aaacttttgc ctgagccgca
      301 aaagttacta aaagtgctgg cagtcgcccc aacaggctca caggaaatct tcccattggc
      361 ggtcaatgct cttcaatatt tttatttgat tgcgggtgtt ttcgggggag gagggctttt
      421 aatttcaacc ctgtcttttg catctttgat cgcatctgga gtttttggca ttgagtacat
      481 ctctcaaatc tttgttgcta tcttaaatgg tgcattttta tttcttgttg ccttttctgc
      541 tgtacggggt cttcgcactc gtcttggcgg ctcgcttgca ctataccctt tattgatttt
      601 aatgggcttt gtgtatctta ttaacttcgc taatttttcc gccttgtttg ttgattccaa
      661 ttcagaggag acttcacagc tggcatttac ggctgtcaat ggggctgggt ttgccttgtg
      721 gtttttaccg atgggcacat tcctaataat tcccttcctt ctttttggct ggcagggctt
      781 atatcgttgg attccgcttc gccaaaccga taatgtcacc tcgttggaaa gtatattttt
      841 attctatgcc ccccggaaaa aagggaggct tgttttccac attttgtttc acttgctctt
      901 agcagttatt gtgattgcag ctatgcaatt ggattcaggt gaaatactca ctgatttgct
      961 agtagtagtt atgatggcag tattttttcg ctggctggcg aggcgtaatt taaaataaac
     1021 attgccttga atgaacaata aatacccacg ccgttcttct tcaagaacgg cgtgggtatt
     1081 tatgtaggct ttttaaacga gttctacagc ttaccgaacg ctaccccttt gtagttccaa
     1141 ccacaagccg ctacggcttc ctgaatcggc ttcttgattt cattcgtatt aaagcgatag
     1201 ttgtagacca atcccggctt caattcttcg gtaaacgcat aggctgcacc aaattccacc
     1261 gaagtcatct gtccctgaaa cttcgagacc gagatcgaaa gcgacggcac gcctgcccgc
     1321 cattccacgg tgtattcttc atctttagca cgtacttcgt tctttgccgg gtcgaacttc
     1381 atgtaaatgc gaaagacctt ggtgatgttt gctttcgcaa agagctcata ccattttgca
     1441 tccacgatct tccactccgc gatcaagtcc acacctttgt ctttcccgtc aataatttga
     1501 tacggcgccg tttcacggtt caattccaat atcttcgtcc gcacttcgtc tgctgagaga
     1561 accggtgcgt tcttgtttgc tttcttgccg aaaccaaaga gtcccataac aatgtccttt
     1621 cgaattgatg aactgacttt attatacgag ttgcttccac attctctatc ccccagatag
     1681 gcgattggg
//
LOCUS       DAOREF010000757         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_63612_length_1684_cov_4.874156, whole genome shotgun sequence.
ACCESSION   DAOREF010000757 DAOREF010000000
VERSION     DAOREF010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63612_length_1684_cov_4.874156"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..261)
                     /locus_tag="PKI33_13925"
     CDS             complement(16..261)
                     /locus_tag="PKI33_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNM38163.1"
                     /translation="MTTEIITPIAKFIAEKILKQPSKVISPDEALISSGLIDSFSLMD
                     LALFIEDTFGVRVEDTELNAETFDNLNQLATLISSRK"
     gene            complement(416..1345)
                     /locus_tag="PKI33_13930"
     CDS             complement(416..1345)
                     /locus_tag="PKI33_13930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNM38164.1"
                     /translation="MTVRQLFLLSILLLAACTTAPTATAQPENQSTSTPEATETETAI
                     AATEESAPTPRPTLRPDEWMTLPIIPEVTDTAREIYKRGQELGRDPTHFSKVGDCQTN
                     TGFYLVDFDHEGWYSLGEGYAYLQDTIDYYQGSYSRTSLAMRDGYNVAAILTPLRADP
                     KKCEKGENPIQCEFRLHNPSIAIISLETNFNDRPADDYGKYMRQIIEYSIEQGVVPIL
                     ATKGDNVEGDNSINAEIAKIAVEYDIPLWNLWAALQPLPNQGHDTDLNDGFHLSFSRN
                     FFDKPKNMLSGWPWRNLTALQALDAVRRGLQEQ"
     gene            complement(1440..>1684)
                     /locus_tag="PKI33_13935"
     CDS             complement(1440..>1684)
                     /locus_tag="PKI33_13935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNM38165.1"
                     /translation="MLISSGANSDIRYNFFYPRWAYDDYREIMSSLSERNDWTYVDLW
                     DIVPANEFTNSAIHLTPAGENMLAENLAPYILESCK"
BASE COUNT          385 a          379 c          447 g          473 t
ORIGIN      
        1 acgtgaatta attatttatt tgcgtgagga aatgagtgtg gcgagttgat taagattatc
       61 aaacgtctct gcattcaatt cggtatcttc cacgcggacg ccgaaggtgt cttcaatgaa
      121 gagcgccaag tccattaggc tgaatgagtc gatcagtccg ctggagatga gggcttcatc
      181 cggagaaata actttgctcg gctgcttgag aattttttct gcgatgaatt ttgcaatggg
      241 ggtgatgatt tcagttgtca tgaatgattc ctttttgtat aaactcggtg gttgagtagc
      301 ccgaggcgac agccgagggc gtatcgaaac cacctgtaaa tagaatattt tttgttaatt
      361 ggtggtttcg atacgggcga gaagagcact cgccctactc aaccaccggg ttgccttact
      421 gttcttgcaa ccctcgtctt accgcatcca acgcctgcaa tgccgtgaga ttgcgccagg
      481 gccagccgct gagcatgttc ttgggcttat cgaagaaatt gcgcgagaac gagaggtgga
      541 aaccatcgtt cagatccgtg tcatgaccct gattgggcag aggctgaagt gccgcccaca
      601 gattccataa cgggatatcg tattctacag caatttttgc gatttctgca ttgatactgt
      661 tatcgccttc gacgttgtcc cctttggtcg caaggatggg aaccaccccc tgctcaatgg
      721 aatattcaat gatctggcgc atatacttgc cgtaatcatc agcagggcgg tcattgaaat
      781 tggtctcaag gctgataatg gcgatgctcg ggttatgcaa gcggaattcg cattgaattg
      841 gattttcgcc tttttcacac ttcttcgggt ctgcgcggag cggggtaagt atggcagcta
      901 cattatatcc atcacgcatg gcaaggctgg tccgggaata agagccttga tagtagtcta
      961 ttgtatcttg caggtaggca tagccttccc ctaaactgta ccaaccttca tgatcaaagt
     1021 ctacgagata gaaaccggta tttgtttgac aatcgccaac tttggaaaaa tgcgttgggt
     1081 cacgcccgag ttcttgtcca cgtttgtaga tttcccgtgc ggtatcggtt acttctggga
     1141 tgatgggtag ggtcatccac tcatcgggtc ggagcgtggg gcgcggcgtc ggcgcggatt
     1201 cttcggtggc tgctatggct gtttcggttt ccgtcgcttc gggcgtggat gtggattggt
     1261 tttcaggttg agccgttgcc gttggcgccg tggtacaagc ggcaagcaac aaaatagata
     1321 aaagaaaaag ttgtcgtact gtcatttatg ctccggattg tcaccctgag cgatagcgaa
     1381 gggtctctga gactatctaa gagatgcttc gctgtcgctc agcatgacat tgaatttttt
     1441 tatttacaac tttccaaaat atacggcgcg aggttttctg ctagcatgtt ctctcccgca
     1501 ggcgtgagat gaatggcgct gttggtgaat tcatttgcgg gcacgatgtc ccacaaatcc
     1561 acatacgtcc agtcattgcg ttcgctcaac gaactcatga tctcgcggta atcgtcatac
     1621 gcccagcgcg ggtagaaaaa attatagcgg atgtcgctgt tcgcgcccga acttattaac
     1681 atcg
//
LOCUS       DAOREF010000758         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_63982_length_1679_cov_3.529557, whole genome shotgun sequence.
ACCESSION   DAOREF010000758 DAOREF010000000
VERSION     DAOREF010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63982_length_1679_cov_3.529557"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..898)
                     /locus_tag="PKI33_13940"
     CDS             complement(59..898)
                     /locus_tag="PKI33_13940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM38166.1"
                     /translation="MRKTVLITGSTTGIGKAGALELGRRGWQVFVHARTEERGKTVLD
                     ELKNSAPNANFSLVTGDVSSMEQVKSLAKQVKEQTDTLDVLWNNAGLMLNERQVSVDG
                     WEKTMAINHLAPFVLTRELIPLIESVQGRVITTSSGAHYFGSFNWDDWMDEKSKYNSF
                     KIYSKSKLANVLFTRELAKRIDAKGITAHCFHPGMVRTDFGKQTRGVPSQGSWYPRWM
                     EALFTIDTEAGADTGVFLAEDPSAKNSNGKYWFKRKVRRTNRFDNDQNAAKLWELSEK
                     ATN"
     gene            complement(1049..>1679)
                     /locus_tag="PKI33_13945"
     CDS             complement(1049..>1679)
                     /locus_tag="PKI33_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNM38167.1"
                     /translation="MIGSLAAERRIKPQDDLITELVQVENQGDKLTEDELRATAIFLL
                     NAGHEATVNGSSLGLRALFQQPDQLKALKEAVAQGNTSLLKTGVDEMLRYETPLPMFE
                     RYVLEDMDFNGVKLERGMEVALMYISGNRDEARFTQPNELILSREDNPLLTFGLGTHY
                     CLGAPLARLELQVLFSVLFTRCPNIRSAGEAEFSSGFVIRGLNKLPIEF"
BASE COUNT          411 a          410 c          399 g          459 t
ORIGIN      
        1 agtataactt aacaatgaaa tcatccccgc attcctgacg gggatgattt cataataact
       61 agttggttgc tttttcgcta agttcccaaa gtttagcggc attctgatca ttgtcgaagc
      121 gatttgtgcg gcggactttg cgcttgaacc aatattttcc attactgttt ttagcagatg
      181 ggtcttctgc aagaaataca ccagtatcgg caccggcttc agtatcaata gtgaatagtg
      241 cttccatcca gcgtgggtac caagaacctt gagatggcac tcctctagtt tgtttgccga
      301 agtcagtccg caccatgcct gggtggaaac aatgagctgt gatgcctttt gcgtcaatcc
      361 gcttcgctaa ttcgcgggtg aaaaggacat tggcaagttt acttttgctg tagattttga
      421 acgagttata tttgctcttc tcgtccatcc aatcatccca gttgaaagac ccaaagtaat
      481 gagcgccaga actggtggtg atgactctgc cttgaacgga ttcgatgagc gggatcaatt
      541 cacgagtcaa tacgaacgga gcgagatggt taatcgccat ggtcttttcc catccatcca
      601 cgctgacctg gcgttcattc aacatgagtc ctgcattatt ccaaagaaca tccaaagtat
      661 cggtctgttc cttgacctgt ttggcaagag atttgacctg ctccatcgaa gagacatcgc
      721 cagtgacaag cgagaaatta gcgttcggcg cgctgttctt caactcgtct aacacggttt
      781 ttccgcgctc ctccgtacgg gcatggacaa atacctgcca accgcgacgt cccaattcca
      841 atgcgcctgc tttaccaatg cccgtggtcg agcctgtaat caaaactgtt tttctcataa
      901 taatttcctt ttgcgatgaa ttttcacctt cgtgtagatt gaatgtaaga aaaaaatctt
      961 tctctgaata attaataatt ttgtgagaat aggtttgcaa caaaaaagca gccttttggg
     1021 gctgcttttt tgttgacaca aggtgtcatt aaaattcgat gggtaatttg ttcaacccac
     1081 gaatgacaaa gccggaagaa aattccgctt ctcccgccga gcgaatgttc gggcagcgtg
     1141 tgaacaacac actgaacagc acctgcaact ccaatcgtgc gagcggagcg ccgaggcagt
     1201 aatgggtgcc gagaccaaac gtcagcagag ggttatcttc acgagacagg atcaactcat
     1261 tcggttgtgt gaagcgggct tcatcgcgat tgcccgaaat gtacatcaat gccacttcca
     1321 tgccgcgctc cagcttgacg ccgttgaagt ccatatcttc gaggacgtaa cgctcaaaca
     1381 tcggcagcgg agtttcgtag cgtaacattt cgtcgacgcc tgttttgagc agggatgtgt
     1441 tgccttgagc gaccgcctct ttcaacgcct tgagttggtc gggttgctgg aagagcgccc
     1501 tcagcccaag cgaagacccg ttgaccgtgg cttcgtgccc ggcattgagc aggaagatag
     1561 ccgtagctcg taattcatct tcggtaagtt tatcgccttg attctccact tgcactaatt
     1621 cagttatgag atcatcctgt ggtttgatgc ggcgttcggc ggcgagcgaa ccgatcatc
//
LOCUS       DAOREF010000759         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_64203_length_1676_cov_5.649599, whole genome shotgun sequence.
ACCESSION   DAOREF010000759 DAOREF010000000
VERSION     DAOREF010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64203_length_1676_cov_5.649599"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PKI33_13950"
     CDS             complement(<1..1023)
                     /locus_tag="PKI33_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNM38168.1"
                     /translation="MNLVHGVDVSYWEPKIDWQALRSQGFKFALIRATSGVGYVDPKF
                     KSHWEGARKEGLLRGAYHYLIANQDPKQQADLFISNISADRGELPPIIDLEDKYNESA
                     KNTQIIDTCKAILDRVEKAFGVKPMIYSRTQYLNLHVSRNGAAPSWAKDYPLWLAQYP
                     YVFDANTMPNKNMPTQATGWQDWLFWQYSETTLVDGVTSDNGQPTECDLNWFRGTEEE
                     LYAFANVKPKEPVSYTVKSGDTFKAIAEKYKITVDELLAANPQLLADGTKLSIPVPTI
                     SKPIVSDNSTTNKATSSTRTHTVKQGDTIGTIAQKYGVTVDAIKALNPQLTNPNLIYV
                     GQVIVIP"
     gene            1152..1328
                     /gene="ssrS"
                     /locus_tag="PKI33_13955"
     ncRNA           1152..1328
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKI33_13955"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          428 a          418 c          392 g          438 t
ORIGIN      
        1 cggaataacg atcacctgcc ccacataaat caaatttgga ttggtcaatt gcggattgag
       61 agctttgatg gcatccacgg tcacaccgta tttttgagcg atggtgccaa tcgtatcgcc
      121 ctgctttacg gtatgtgtcc gcgtggagga agttgcctta ttcgtcgtgg aattatcgct
      181 gacgattggc ttcgaaattg tcggcacagg gatggaaagt ttcgtgccat ccgccagtaa
      241 ctgcggattt gccgccagca attcatccac cgtgatctta tatttctcgg cgatcgcttt
      301 gaaggtgtcg ccacttttca ctgtgtagct gaccggttct ttcggcttga cattcgcaaa
      361 tgcgtagagt tcctcttctg ttccgcggaa ccagttcagg tcacattcgg tgggctgacc
      421 attatcactc gtcacaccat ctactagcgt agtttcagaa tattgccaga agagccaatc
      481 ttgccaacct gtcgcctgag taggcatgtt tttatttggc atggtattgg catcaaacac
      541 atacggatat tgcgccaacc aaagcggata atccttcgcc cacgaaggcg ccgctccatt
      601 ccgtgagaca tgcaaattca aatactgagt gcgtgaatag atcattggtt ttacaccgaa
      661 cgccttctcc acccggtcta gaatcgcttt gcatgtatca atgatctgcg tgtttttagc
      721 actctcattg tatttatctt caaggtcaat aataggcggc agttccccac ggtctgcact
      781 gatgttggaa atgaaaaggt ctgcttgttg tttcgggtct tggttggcga tcaggtaatg
      841 atacgcccca cgcaagagac cttctttgcg tgctccttcc caatgagact taaacttggg
      901 gtccacatac cccaccccag aagtggcacg aatcaaagca aacttaaacc cctgagaacg
      961 cagagcctgc caatcaattt taggctccca ataacttacg tcaacgccat ggactaaatt
     1021 cattgttctc tcctttgaat aaaaacactg ctcaaattag cctagcacaa ttttttgaga
     1081 aaatcaatct cttcgttaag agcatactta gagagtatct ttccttgtct cgcagagtat
     1141 ggtataagaa tcgcaccctg cggtgctcgt gatatcgccc ttccgttttg agccaatact
     1201 cactaaaagg gtccttaact ttacagtaac aacgcgatag gcctgagcca aggcggcgtt
     1261 gcttggtagg gacccacctg caatagcagg ttcaaaaccc cgcggtacac ggcacaggcg
     1321 gggtacgcaa ttatgaagcg gaatgttccg tagaggaaca ttccgctttt tagtggtcta
     1381 caccacttgc ttgacgaaac acatctgctc catgtaaaat ctcgcccgtt gttaggggta
     1441 actacaaaaa aataatggag atacaagcgg ctgggtctcg ttgtacacga gatcacgagg
     1501 tggaggttct cccgtgcgaa cgggatgcta aagacctagg ggtgaggtct aaaaatcgga
     1561 tagatcagcg gatacctctg aagccagaat cactggcttc cttctccctt ccaaagcaag
     1621 cgcaaacaga aaaccgcacg gcgacgagcg ggacaatgga agcgcagtga tcaggt
//
LOCUS       DAOREF010000760         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_64278_length_1675_cov_4.154321, whole genome shotgun sequence.
ACCESSION   DAOREF010000760 DAOREF010000000
VERSION     DAOREF010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64278_length_1675_cov_4.154321"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKI33_13960"
     CDS             <1..357
                     /locus_tag="PKI33_13960"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNM38169.1"
                     /translation="NFSYYMRVLKKDTGELIGQIIDISTGGFKLESSKPIPSNVQMEL
                     CIDQVNQISPRSYIVFSARTRWCDRDPYDPTIYNVGFQLVDMTPADYDVFVTMFNTYG
                     EKKQAHHKTNTDYIWG"
     gene            445..1113
                     /locus_tag="PKI33_13965"
     CDS             445..1113
                     /locus_tag="PKI33_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNM38170.1"
                     /translation="MKRLIVWFIRVVIHLVGKVDASGYENVPEKGGFVIAVNHLGFLD
                     APMAYYALDNWNLFIPVAEKWEESAILRWLGKHLNAIFIDRFNPDLKAMREMIHRMEQ
                     GQTLVIAPEGTRARDEKMAQGKPGVAYLASKMGWTIVPVAISGTEDRIVIQNLKKLKK
                     SSIKLTAGKSFVLPPFPKENREEKLQDYTDEIMCRIAVMLPEHNRGYYAEHPRLKELL
                     VENK"
     gene            1110..>1675
                     /locus_tag="PKI33_13970"
     CDS             1110..>1675
                     /locus_tag="PKI33_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNM38171.1"
                     /translation="MSAFLRFCARKVLPLIADIEVNGDISKLPKEGGYMLASNHLGRL
                     DSLAVYYVLDNDDLIHPLTDKYKKYWYGRLAGKIFNVTWLTRGEADMSAIKEFISRLK
                     KGAVMIIAPEGTRSKSVSLLKAEPGAIYIASSANVGIIPVALTGTEDADVVARLKSFR
                     RLKITISTDGEIYTPPNIKAAKGAERDA"
BASE COUNT          473 a          337 c          422 g          443 t
ORIGIN      
        1 aatttttcct attacatgcg cgtgctgaaa aaagatactg gtgaactgat cggtcagatc
       61 attgatatta gcacgggcgg ttttaagctg gaaagcagta agccgatacc aagcaatgtg
      121 caaatggaac tttgtattga tcaagtgaat cagatttcac ctagaagtta cattgtgttt
      181 tctgcccgaa cccgatggtg tgaccgcgac ccgtatgacc ccacaattta caatgttggg
      241 tttcagcttg tggatatgac cccggcagat tacgatgtct ttgtaactat gttcaatacc
      301 tatggagaaa agaaacaggc tcatcataag accaatacag attacatttg gggttgatct
      361 ttagcgaacg ttcaagccat gttttacgaa acatggcttt tttgtttaaa cttcccagaa
      421 ataaaaccgt gtaaaattgc cgccatgaaa agattaatcg tctggttcat tcgagtggtt
      481 attcaccttg ttggcaaagt ggacgcgagc gggtatgaga acgttcctga aaagggcggt
      541 tttgtcattg ctgtaaatca tttgggtttt ttagatgcgc ccatggcata ttacgccttg
      601 gataattgga atctttttat tccggtggca gaaaaatggg aggagagtgc catcctacga
      661 tggttgggaa aacacctcaa tgccatcttc attgaccgct tcaacccgga cttgaaagcg
      721 atgcgtgaaa tgatccaccg catggaacag ggacagactc ttgttattgc tcccgaagga
      781 actcgcgctc gcgacgagaa gatggcgcaa ggcaaaccgg gtgttgcata tcttgcctcc
      841 aaaatgggtt ggacgattgt gccagttgct atcagcggca ccgaagaccg cattgtgatt
      901 cagaatctca aaaaactgaa gaagtcatcc attaaactca cggctggaaa atcttttgtc
      961 ttgccgccat tcccaaaaga gaaccgcgaa gaaaaattgc aggattatac tgacgagatc
     1021 atgtgccgca ttgccgtgat gctaccggaa cataatcgcg gctattatgc ggagcatccg
     1081 cgcttaaaag aattattggt tgaaaataaa tgagcgcctt ccttcgcttt tgcgccagaa
     1141 aagttttacc gctgattgcg gatattgaag ttaacggcga tattagcaaa ctgcccaaag
     1201 aagggggata catgttggca tccaatcact tgggtcggtt ggattcgctc gctgtatatt
     1261 atgtgcttga taatgatgac ttgattcacc cattgacgga taaatacaaa aaatattggt
     1321 atggacggtt agcggggaag attttcaacg tcacatggtt gacgcgcggt gaagcggata
     1381 tgagcgctat caaggaattt atttcgcgct tgaaaaaggg ggcggtgatg attattgccc
     1441 ctgaaggcac gcgctcgaag tcagtgagtt tgttgaaagc cgaaccgggc gcgatttata
     1501 tagcgagttc ggcgaatgta gggattatcc cagtggcatt aacaggtacg gaagatgccg
     1561 atgtggtggc gagattgaaa tcattcagac gtttgaagat caccatttct actgatgggg
     1621 aaatatacac tccgccgaat atcaaagccg ccaaaggcgc agaacgggat gcatt
//
LOCUS       DAOREF010000761         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_64507_length_1672_cov_4.211503, whole genome shotgun sequence.
ACCESSION   DAOREF010000761 DAOREF010000000
VERSION     DAOREF010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64507_length_1672_cov_4.211503"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1672)
                     /locus_tag="PKI33_13975"
     CDS             complement(57..>1672)
                     /locus_tag="PKI33_13975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM38172.1"
                     /translation="AIQNARLFEEARLARAAAEQANEAKSAFLATMSHEIRTPMNAVI
                     GMSGLLLDTELNDEQRDYVETIRGSSDTLLAIINDILDFSKIEAGRMDLEHQPFDLRE
                     CVEASLDLVTARAVEKSIDIAYIFEGDIPPAIVGDVTRLRQVITNLLSNAVKFTDRGE
                     VVLTVSSQRYKNGSAKEKVMLTFAVRDTGIGLTHEGMSRLFQSFSQADSSTTRKYGGT
                     GLGLAISKRLAEMMGGTMWAMSQGLGLGSTFMFNIKAEVADLPPAKKQIHLGIQPALQ
                     NKRVLIVDDNATNRYILNMQTAKWGMVPRDTAFPAEALQWIENGDFFDIAILDMHMPE
                     MDGLELAKHLREKQMKFPLVLFSSLGRREAGDETNLFSAYLSKPIKQSQLFDTLVGLF
                     VQDEEENKKVTTDRFKPDPQMAARHPLRILLAEDNAVNQKLALRLLEQMGYQADVAVN
                     GRETVQAVERKEYDVILMDVQMPEMDGLEATREIVTRWAEKHPYIIGLTANAMQGDRE
                     MCLNAGMDHYIPKPIRVVELVDALFKAKS"
BASE COUNT          382 a          452 c          398 g          440 t
ORIGIN      
        1 tcaattcatt gaaggttttc atatccaaaa caggcatagg tctccaggtt ttcttcttac
       61 gacttcgcct tgaataaggc atctaccaac tctacgacac ggatgggttt gggaatgtaa
      121 tgatccatcc cagcattcaa acacatctca cggtcacctt gcatggcatt ggcggtcagc
      181 ccaataatgt aggggtgttt ctccgcccag cgtgtcacga tctcacgggt cgcttccaac
      241 ccgtccattt cgggcatttg cacatccatc aaaataacgt catactcttt acgctccaca
      301 gcttgcactg tttcacgtcc attcactgcc acatctgcct gataccccat ctgctccaac
      361 aaccgcaacg ccaacttttg attgacggcg ttgtcttcag cgagcagaat tctcaacgga
      421 tgccgcgccg ccatttgcgg gtccggtttg aagcggtctg tcgtcacctt cttattctct
      481 tcttcatcct gcacgaagag tcctaccaag gtatcgaaca gttgcgattg cttgatgggt
      541 ttcgaaagat aggcagagaa gagatttgtc tcatcgccgg cttcacgcct gcccaacgaa
      601 ctaaacaaca ccaacgggaa tttcatttgc ttttcacgca aatgctttgc caattccaag
      661 ccgtccattt caggcatgtg catatcgaga atggcgatgt cgaaaaaatc accattttcg
      721 atccactgca aagcttcagc agggaaggca gtatcacgcg gaaccatgcc ccacttggca
      781 gtttgcatat tgagaatgta acgattggtg gcattgtcat ccacgatcaa tacgcgtttg
      841 ttttgcaatg ccggttgaat gccaagatga atttgtttct tggcgggggg caggtctgct
      901 acttcggctt tgatgttgaa cataaaggtt gagcccaacc ccaagccctg gctcattgcc
      961 cacatcgtac cgcccatcat ctcagccaag cgtttgctaa tggcaagccc caaccccgtg
     1021 ccgccatact tgcgcgtggt agatgaatca gcttgcgaga acgattggaa gagccgactc
     1081 ataccttcgt gtgtgagacc gatacctgta tcgcgtactg cgaacgtgag catgactttc
     1141 tctttcgcgc ttccgttctt gtaccgttgg ctggataccg ttaaaacgac ctcacctctg
     1201 tctgtaaact tgaccgcgtt actgagcaaa ttggtgatga cctgccgtaa gcgagtcaca
     1261 tcgcccacaa tagcgggcgg aatatcgcct tcgaagatat atgcaatgtc gatgcttttt
     1321 tctacagccc gcgccgtgac caggtctagc gatgcttcga cacattcacg caggtcaaag
     1381 ggctgatgtt caaggtccat gcgtccggct tcgatcttcg agaagtcgag aatgtcgttg
     1441 atgatggcaa gcaaggtatc gcttgagccg cgaatggtct caacataatc acgctgttcg
     1501 tcgttgagtt cggtatcgag caaaagtccg ctcatgccga taacggcatt catgggcgtg
     1561 cgaatttcat ggctcatggt cgcgaggaag gcgctcttgg cttcgttggc ttgttctgcc
     1621 gcggcacgtg ccaaacgcgc ttcttcgaac aggcgtgcat tttgaatggc tg
//
LOCUS       DAOREF010000762         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_64728_length_1669_cov_2.834572, whole genome shotgun sequence.
ACCESSION   DAOREF010000762 DAOREF010000000
VERSION     DAOREF010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64728_length_1669_cov_2.834572"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKI33_13980"
     CDS             <1..688
                     /locus_tag="PKI33_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNM38173.1"
                     /translation="LLGWRFLGGFKFAMRTAVAIVTYSIFTDLVMKIPAFVSYSQILI
                     NDLQGDIFLNSLYGAIISGAGYGLVYRARGTSGGSDILARILNNWRGISMTQSYLMVD
                     AVVILSAGFVFGWKAALYAMITLYVSGLVAETVLEGGGTVRTAMIVTSQPDDVSNRVL
                     EEMGRGVTWLEGKGGYTGNSKPVLYCVVSRAEVATLKAIVHESDPESFLVIGTAHEAW
                     GEGFKPLKGR"
     gene            complement(723..1631)
                     /gene="era"
                     /locus_tag="PKI33_13985"
     CDS             complement(723..1631)
                     /gene="era"
                     /locus_tag="PKI33_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNM38174.1"
                     /translation="MSQTNHRSGFIAIIGRPNVGKSTLLNALLGQKIAAVSPRPQTTR
                     KRQLGILTLDNAQLVFVDTPGIHNAKHKLGEFLNAEAEEALEGVDLILWIVDASTSPT
                     EEDKNISSLLYGISPRTRRMLILNKSDLVQPDELSAREKEYAALMDKASVMHVSAARR
                     LGLGELTEALIQAVPEREADYPEDQITDLYEKEIAADLIREACLIKLRDEVPHSMAIR
                     IDEFKERENGMAYIAATIFVERESQKGIVIGEGGKMLKTIGSAARKEIEDMGGHKVYL
                     ELRVKVSKDWRNDANTLKQLGYGMKK"
BASE COUNT          343 a          415 c          456 g          455 t
ORIGIN      
        1 tcttttgggt tggcgtttcc ttggcggatt caaattcgcc atgcgcactg ccgttgccat
       61 tgtcacctat tcgattttta cagatctggt gatgaagatt ccggcttttg tctcttattc
      121 tcaaattctg atcaacgacc tgcaaggcga tattttcttg aattcactct atggcgccat
      181 tatcagtggt gccgggtatg gattggtcta tcgcgcacgc ggtactagcg gtggatcgga
      241 tattctggcg cgcatcttaa acaactggcg cggcatttcg atgacccaaa gctatctaat
      301 ggtggatgcg gttgtgatcc tctcggcggg ctttgtcttc ggctggaagg cggcgttgta
      361 tgccatgatc actctgtacg tcagcgggct tgtggcagaa acagtgctcg aaggcggcgg
      421 caccgtccgc acggcaatga tcgttacctc acagccagat gatgtttcta atcgcgtctt
      481 ggaagagatg gggcgcggcg tgacctggtt ggaaggcaag ggcggctata ccggcaattc
      541 gaaacctgtc ttgtattgtg tggtcagccg ggcggaagtg gcaacgctca aagccattgt
      601 ccacgagagt gaccctgagt catttctcgt cattggtaca gcacacgaag catggggtga
      661 gggcttcaag cctctcaaag ggcgctaata aaaaaactcc gaggctacaa cctcggagtt
      721 ttttacttct tcattccata tcccaattgc ttgagagtat tcgcatcatt acgccagtct
      781 ttcgagacct tcacgcgcaa ctccaaatat actttgtgcc cgcccatatc ttcgatctct
      841 tttcgcgcgg cacttccgat ggtcttcaac atcttgccgc cttcgccgat gacgattcct
      901 ttttgagact cgcgctctac aaaaatggtg gcggctatgt atgccatgcc attctcgcgt
      961 tctttgaatt cgtcaatgcg gatcgccatg ctgtgcggaa cttcgtcgcg caatttgata
     1021 agacaagctt cacggatgag gtcagctgcg atttcctttt catataaatc agtgatctga
     1081 tcttcgggat aatccgcctc gcgctccggg acggcttgaa tcagcgcttc ggtcagttcg
     1141 cctagcccca gcctccgtgc cgcagagacg tgcatcacac tggctttgtc cattaatgca
     1201 gcatactctt tttcgcgggc agacaattcg tcgggttgca ccaggtctga tttgtttagg
     1261 atcagcatgc ggcgagtccg aggcgagata ccgtaaagca aggatgagat attcttgtcc
     1321 tcttcggtcg gggaggtgga tgcatccacg atccaaagaa ttaaatccac gccttcgagg
     1381 gcttcttcag cttcggcatt gagaaattca ccgagcttgt gcttggcatt gtgtatgccg
     1441 ggtgtatcga caaagaccag ttgggcattg tcgagggtga gaatgccgag ttgacgcttg
     1501 cgggtggttt gcgggcgcgg cgagaccgca gcaattttct gcccgagcag tgcattcagg
     1561 agggtggact tgcccacgtt ggggcgtccg ataatggcga taaagccgga acggtggttg
     1621 gtttgactca tgcaggtttc ttctgatagt ttacgatgac gagactcat
//
LOCUS       DAOREF010000763         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_64786_length_1668_cov_3.730936, whole genome shotgun sequence.
ACCESSION   DAOREF010000763 DAOREF010000000
VERSION     DAOREF010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64786_length_1668_cov_3.730936"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKI33_13990"
     CDS             <1..516
                     /locus_tag="PKI33_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38175.1"
                     /translation="YGGTGGLVVVQGNADSRACVRLSGADVIIGGEITKPLNDSLGFI
                     GARANVKGFLCEYMTAGRVLVLGDPGPWMCAGMTGGVLYLRLQPHLHFDYPAIQRRLA
                     KGAKVSVQAVDDADFQNLQHLLTIYAEELRQNHQADEADNVLGLLIDWKNTFIRVVPE
                     GLQEDQRFATE"
     gene            746..>1668
                     /locus_tag="PKI33_13995"
     CDS             746..>1668
                     /locus_tag="PKI33_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNM38176.1"
                     /translation="MIFTIIIFLLAYIAIASEKFPRHWVALLGGSLLILFGVLTPSEA
                     LSYISWETLGLVAGMFTLVSILVNAGFFTWLAMTSLRKVNYHPVPLFIILILLAAFLA
                     AFMDSITVMLFLSALTLQLCTLLKIDPVPLVIAEVCAANTGGAATLVGDPPNVILGTT
                     LGFSFADFATNTGPIAIVGALILMAYAYIKNRKSLKEAHIALTPEMIDQIDELHNEPL
                     HVHLTRVGLTGFMMAVILLIFHIPLSTLTGLPINAAVSALIPAGIALIALRDDQRKKV
                     IISVDGESILFFAGLFMLIGGLEKVHLFETLA"
BASE COUNT          380 a          428 c          391 g          469 t
ORIGIN      
        1 tatggcggca caggcggctt ggtcgttgtg caaggtaacg cggactcacg cgcctgtgtg
       61 cgtctctccg gtgcggatgt catcatcggc ggcgagatca ccaaaccact caacgacagc
      121 ctcggcttta tcggagcgcg cgccaacgtc aaaggcttct tgtgtgaata catgaccgcc
      181 gggcgtgtgc tcgtactcgg cgaccccggt ccgtggatgt gtgccggtat gaccggcggc
      241 gtgttgtatc tgcgcttgca accgcatttg cattttgact atcccgccat tcaacgtcgc
      301 ctcgccaagg gcgcgaaggt ctctgtacaa gcggtggatg atgccgactt ccagaacttg
      361 cagcacctgc tcaccatcta tgctgaagag ttacgccaaa atcatcaagc cgacgaagct
      421 gataatgtgc tgggtctgtt gatcgattgg aagaatacct ttatccgtgt cgtgccggaa
      481 ggtttacagg aagatcagag attcgctacc gaataacaac gtacccaaac gggtgaaatt
      541 aaatcaactg cgccatgaga aattgtttca tggcgcggtt gtcatttaaa cgtgctccat
      601 ttggggcatc ttttcaatgt gatctatagt tgtttgagca caacatgaaa tgaataccca
      661 ttagggtaat agccatacag gttcaattcc atacaataag gcgcattaca taaaacgtta
      721 ttcatctttg aggagagatg ccgctttgat attcaccatc atcatctttt tgttggcata
      781 tatcgcgatc gcaagtgaga agtttccacg ccattgggtt gcccttttgg gtggttcact
      841 tctaatcttg tttggtgtcc tcactccatc agaagctctt tcatacatca gctgggaaac
      901 tctaggtttg gttgctggga tgtttaccct cgtatccatt ttggttaacg ctggcttttt
      961 tacttggtta gccatgacat ctcttcgcaa agtcaattac caccctgtgc ccttattcat
     1021 tattttgatc cttttggctg cattcctggc agccttcatg gatagcatta cagtaatgct
     1081 gttcctttct gcattgacat tacaactctg cactttgttg aagattgacc ccgtcccact
     1141 tgtgatcgca gaagtctgtg ctgccaacac cggtggcgca gccacattgg tcggcgaccc
     1201 tcctaatgtt attctaggaa ctacattggg gttcagtttt gctgatttcg ccacaaatac
     1261 cggtccaatc gccattgtcg gcgcgctgat cctcatggca tatgcctata tcaaaaatcg
     1321 caaatcgttg aaagaagctc acatcgcgct cacaccagag atgatcgacc agatcgacga
     1381 attgcataat gaaccgcttc atgttcacct gactcgtgtc gggctgaccg gttttatgat
     1441 ggcagtcatc ctcttgatct tccacattcc cctaagcacg ctaaccgggc tacccatcaa
     1501 tgcggcagta tcggctttga tccctgctgg catcgcactg atcgccttaa gagatgacca
     1561 gcgcaagaaa gtcattatca gcgtagacgg tgaaagcatt ctattctttg ccggtctctt
     1621 tatgctcatt ggcgggctgg agaaagtcca tctcttcgaa actcttgc
//
LOCUS       DAOREF010000764         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_64879_length_1667_cov_2.405707, whole genome shotgun sequence.
ACCESSION   DAOREF010000764 DAOREF010000000
VERSION     DAOREF010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64879_length_1667_cov_2.405707"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKI33_14000"
     CDS             <1..602
                     /locus_tag="PKI33_14000"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNM38177.1"
                     /translation="MGEPLVVIEFNADADTVNTIKNMGIPVIHDDGTRPAVLEAANIK
                     KSRTIIMASQNDSMNLQIALKARSLNPKIQVVIRIFDDDFAHALQEQFSFIALSATEM
                     AAPVFASAAAGVDVTNPISVEGQLLSLARLTISKNAPFADKTIGYIEDTYHLSIVLHR
                     KNGHSEMHPTDKTAIHPGDVIAILGGPEQLNKIFHDNGQ"
     gene            592..951
                     /locus_tag="PKI33_14005"
     CDS             592..951
                     /locus_tag="PKI33_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38178.1"
                     /translation="MDSDNSPLIGVVGPCGSGKSTLIRGLEAHGYRCRHIAQEHSYAK
                     QMWQTITKPDILIFLECSFENSTQRRKLNWVAADHEEQLRRLSHAYEHAHIIINTNTL
                     DESGVLAQALAYLKKHL"
     gene            1116..>1667
                     /locus_tag="PKI33_14010"
     CDS             1116..>1667
                     /locus_tag="PKI33_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38179.1"
                     /translation="MRNKNTWLIVILFLLAFSLWVNFSKTITVINPFNDNTPVVERDV
                     DIKLGLDLRGGLQALLEADVPADSTVSSADMENAKNILQNRANALGVSEIVMQTAGDR
                     RIVAEFPGVTNPEEVVSALKQTALLEFVDMGTTPVAEGTTIQTDFAAGDTTAAATPAP
                     DGTTPVIYHTVMTGTSLDTASVVR"
BASE COUNT          445 a          461 c          366 g          395 t
ORIGIN      
        1 aaatgggtga acctttggtc gtcatcgaat tcaatgcaga cgctgataca gtcaatacga
       61 ttaaaaatat gggcattcct gttattcatg atgatggcac acgtccagcc gtgctggaag
      121 ccgcgaatat caaaaagtcg cgcaccatca ttatggcaag ccagaacgac tcaatgaatt
      181 tacagattgc gctcaaagct cgctcgctca accccaaaat tcaggtagtc atccgcatct
      241 tcgacgacga ctttgctcat gcgcttcaag aacaattctc tttcattgct ctaagcgcta
      301 cagaaatggc agcacctgtc tttgcatctg ccgccgctgg tgtagatgta accaacccaa
      361 tttcagtcga aggtcaactg ctaagcctgg cacgcctaac catttcaaag aatgctcctt
      421 ttgcagataa aaccatcggc tatattgaag atacctacca tcttagtatt gtgttgcatc
      481 gcaaaaatgg tcattcagaa atgcacccca ccgacaaaac tgccatccac cctggcgatg
      541 taattgccat tcttggtgga cctgagcaat tgaacaaaat attccacgat aatggacagt
      601 gacaattccc cattaatcgg tgtcgtaggt ccgtgcggtt cgggcaaatc cacattgatt
      661 cgcgggttgg aggcgcatgg ctatcgctgc cggcatattg cacaagaaca ttcctatgcc
      721 aaacagatgt ggcagaccat caccaaaccc gatatcctca tttttctaga atgttctttc
      781 gaaaactcca ctcagcgccg aaagctcaat tgggtagctg ccgaccacga agaacaactc
      841 cgccgccttt ctcacgctta cgaacacgct catataatta ttaacacgaa cacgctggac
      901 gaaagcggag tcctcgccca agccttagct tacctaaaga aacacctcta gccccacgcc
      961 ttgccccgcc tcaggcgtgg acctagtttg gccccaccga ccaagccttt cccacgctgg
     1021 aagcggatcc ctcttcctcc aaaaaatcat tcaaccagag tataatgtcg tccgcgccag
     1081 ccaaaggctg gcattttcgt ggatggagta aacacatgcg taataagaat acatggctga
     1141 ttgtgatcct ctttctgctg gcattctccc tttgggttaa tttcagcaaa accatcacag
     1201 ttatcaatcc ctttaacgac aacacccctg tagttgaaag agatgttgac attaaacttg
     1261 gtctcgactt gcgtggcggc ttacaagcgc ttctcgaagc cgatgttcct gccgattcaa
     1321 cggttagcag cgcagatatg gaaaacgcca agaacattct gcaaaaccgc gcgaacgccc
     1381 ttggcgtgag cgaaattgtg atgcagaccg ccggcgaccg ccgtattgtg gctgaatttc
     1441 cgggtgtaac caacccagaa gaagtggtta gcgctttgaa gcaaacggct ttgctagagt
     1501 ttgttgatat gggcacaacc ccggtagctg aaggtaccac cattcaaacc gacttcgcgg
     1561 caggcgatac aacggctgct gctacccccg cccccgatgg cacgactccg gtgatttatc
     1621 acactgtgat gaccggtacc agcttggata cagcgagtgt ggttcgc
//
LOCUS       DAOREF010000765         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_65081_length_1664_cov_2.904910, whole genome shotgun sequence.
ACCESSION   DAOREF010000765 DAOREF010000000
VERSION     DAOREF010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65081_length_1664_cov_2.904910"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKI33_14015"
     CDS             <1..307
                     /locus_tag="PKI33_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM38180.1"
                     /translation="LIKLDGRLDITGVNQIETKFAAHCSGENARVIVDMAKVDYLASI
                     GIRLLVTNAKSLKSRNGKIIILRPTQSVMEVLEMTNIPAIIPVYTNLESAEAVLLGY"
     gene            430..1461
                     /locus_tag="PKI33_14020"
     CDS             430..1461
                     /locus_tag="PKI33_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38181.1"
                     /translation="MTQKDISFFPKALNLRFEFLKRPVQKTYAPYVFLLLLIIFPPLL
                     IRSKTIYPFYGDLFACIWSAGETYLLFYVAARYRPTSKQLGRAWTIIATSALFSFLGN
                     LFWFGMSHFLHIYPYPSVADIFYLLSYPLMLIGILSFPYTNKTTLEKFKIYLETATVV
                     ISIGMALWFFVLRPVIDQTGTSDKTYLFFTIAYLSVDTLLVMALWVLVTHRSIYLSII
                     TKLQLGMGLLLLILGDTLFLVQDIQGLYFETVRFTDLAYNLSSALIGVAALYQWFTRA
                     EAPTFQITKYWVMNLHRLFSDILLFSSFCLFIYEHENNQFISFETVSFWVGCLILISI
                     VSPTPALPK"
     gene            complement(1371..>1664)
                     /locus_tag="PKI33_14025"
     CDS             complement(1371..>1664)
                     /locus_tag="PKI33_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38182.1"
                     /translation="IPVLKGLNSYVWAGDNYDLVYLRMSDYLSRLEKFGVNPLETGAN
                     LTFNTAWSAKDVGDYPWYPQVRSTYLGKAGVGDTIEIKIKHPTQKLTVSKLMN"
BASE COUNT          429 a          358 c          331 g          546 t
ORIGIN      
        1 actaatcaaa ttagacggca ggctagatat taccggtgta aatcagatcg aaactaaatt
       61 tgcggcacac tgctccggtg aaaatgcacg tgtgatcgtc gatatggcaa aggtggacta
      121 tttggcttct attggcattc gtttgttggt gaccaatgcc aaatccctca agagtcgaaa
      181 tggcaaaatt attattttgc gccctaccca gtccgttatg gaagtgcttg aaatgacaaa
      241 tattcctgcc atcatccctg tctataccaa tcttgaatct gctgaggcgg ttttgctcgg
      301 gtactagctc aatgtaacgg gggaaatata cagaccgatt tactccgaaa tagatgtttt
      361 ccctactagg aatgtttggg gagtcatgtt tgaatttaag tagttgctca gatatgaaat
      421 gagattcaca tgacacagaa agatatttcc ttttttccta aagctttaaa cctcaggttt
      481 gaattcctta aaagacccgt ccaaaaaaca tatgcgccct atgttttctt gctgttgtta
      541 attattttcc cccctctctt aatccgctcc aaaaccattt atccttttta tggtgatttg
      601 ttcgcttgca tttggagtgc tggtgagacc tatcttcttt tttatgtggc ggctcgctac
      661 cgaccaacct ccaaacaatt agggcgggca tggacgatta ttgcaacctc tgccctcttt
      721 agctttttgg ggaatctgtt ttggtttggt atgtcacatt ttttacacat ttatccatat
      781 ccatctgtgg cagatatctt ttacctctta tcttatccgc tgatgttgat cgggatctta
      841 agttttccat acaccaataa gacaactctt gaaaagttta aaatttatct agagacggca
      901 actgtagtta tttcaatcgg aatggcactt tggttctttg tgctaagacc agttattgat
      961 caaacaggga catcagataa gacctacttg ttctttacaa tagcatatct atccgtagat
     1021 acgttactgg tgatggcatt atgggttcta gtgacgcatc gtagtatcta tctttcaatt
     1081 attacaaaac tacaacttgg gatgggactt ctcctattaa tccttggtga tacgctcttt
     1141 cttgtgcagg atattcaagg actatatttt gagacagttc gttttactga tctggcatat
     1201 aaccttagtt ctgccctcat tggcgtggcg gcactatacc aatggtttac ccgggcagaa
     1261 gcgcctacgt ttcagatcac gaaatattgg gtcatgaatc tgcatcgatt attttcagat
     1321 atccttttat tctcatcctt ttgtttgttt atatatgagc atgaaaataa tcaattcatc
     1381 agttttgaaa cagtcagttt ttgggtaggg tgtttgatct tgatctcaat cgtgtcgccc
     1441 acgcccgcct tacccaaata ggtagagcgt acctgtggat accatggata gtcgcctaca
     1501 tccttagcgc tccatgccgt attgaatgtc aaattcgcac cagtttccag tggattcaca
     1561 ccaaatttct ccaggcggct cagatagtct gacatgcgca gatagaccag gtcataatta
     1621 tcacccgccc aaacgtagga gttcaggccc ttgagcaccg gtat
//
LOCUS       DAOREF010000766         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_65271_length_1662_cov_2.070940, whole genome shotgun sequence.
ACCESSION   DAOREF010000766 DAOREF010000000
VERSION     DAOREF010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65271_length_1662_cov_2.070940"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKI33_14030"
     CDS             complement(<1..636)
                     /locus_tag="PKI33_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38183.1"
                     /translation="MTTAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVSAPVRLAK
                     AMIGKSDRRLNQNYVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTEPTVGT
                     VTVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMKRSEIDSKFDEIVDFSEVTQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDV"
     gene            complement(636..1481)
                     /locus_tag="PKI33_14035"
     CDS             complement(636..1481)
                     /locus_tag="PKI33_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38184.1"
                     /translation="MTELTKHEPHTVYIKPSKGIASLNLGDLWIYRELIFFMVWRDVK
                     VKYKQTLLGMAWAVIQPVMTMLIFTFLFGKVAKLPTEGIPYPVFSFTALLPWGLFVTA
                     LNQGSRALVAHQNMVTKIYFPRLILPAASVFAGLVDFAIAFVILVGLMFYYKVTPAWN
                     LVWTLPLFLLLAIVTALGVALWLSAINVQYRDVNQALPFLTQFWLFATPVAYSASVIS
                     EKWQVVYSLNPMAGVVNGFRWALLGTGSGPDISLWVSVGISVLIFVSGLFYFRSMEKT
                     FADTI"
BASE COUNT          466 a          414 c          397 g          385 t
ORIGIN      
        1 aacatcgccc atcttgccca ggcatttttt ctgaaattcg gcatcaccca cagccagcac
       61 ttcatccaca acgaggattt ccggttcgag atgagccgcc acagcaaatg ccaggcgcaa
      121 atacataccc gatgagtatc gctttacggg tgtatcgata aactgggtca cttcagaaaa
      181 gtccacgatc tcatcgaact tcgagtcgat ctcagagcgt ttcataccca ggatggcgcc
      241 gttcatgtag atattctcgc gcccggtcaa ttcagggtgg aagccagttc ccacttccag
      301 cagggagccg acacgtccac gaacagtgac ggtacccacc gttggctcgg taacgcgtga
      361 gaggattttc agcagggtag atttccccgc accgttacgc cctacaatgc caagaacttt
      421 gccttcttct agatcaaaag agacgtcttt caatgcccag acataattct ggttcaagcg
      481 gcggtcagac tttccgatca tcgcctttgc caaacgcaca ggcgcagaga ctgtatccac
      541 gatcacatca cggagcatgt tatagcgaaa tttggtctcc gctgcaccga tcttatattg
      601 cttaccaatg ttttttactg aaatcgctgt tgtcattaga tcgtatccgc aaaggtcttt
      661 tccatgcttc taaaatagaa gagacccgaa acaaagatca aaacggaaat tccaaccgaa
      721 acccacaagg agatatcagg tccactacct gtgccaagca atgcccaacg aaaaccgttg
      781 accacgcccg ccattgggtt gagtgaatac acaacctgcc atttttcaga gatcacagag
      841 gcagaatatg ctacgggtgt ggcgaacagc caaaactgag tcagaaacgg gagggcttgg
      901 ttcacgtcac gatattgaac gttaatcgct gaaagccaaa gcgccacgcc aagtgcggtc
      961 acaattgcaa gcaggaggaa gagtggaaga gtccaaacca aattccaagc gggggtgact
     1021 ttatagtaga acatcaaacc cacgagaatg acaaaggcta tggcgaaatc cactaaaccg
     1081 gcaaagacgg aagcagcagg caggatcagg cgtggaaaat aaattttcgt caccatgttt
     1141 tgatgcgcca ccaaagcacg gcttccctga ttaagcgctg tgacgaaaag cccccacggc
     1201 agcaatgcag tgaaggagaa gacgggatag ggaatgcctt ctgtgggaag ttttgccacc
     1261 ttgccaaaaa ggaaggtaaa gatcagcatg gtcatcacag gttgaataac agcccatgcc
     1321 ataccaagca gtgtttgttt gtactttact tttacatcgc gccagaccat gaagaagatt
     1381 agttcgcgat agatccaaag atcgcctaaa ttgagcgaag caatgccttt ggaaggcttg
     1441 atgtaaacgg tatgaggttc gtgttttgta agttcagtca tatttacttg atcttatcgc
     1501 ggttggcttt gaaccactca atggtgcgct tcatgccttc ttcaaatgaa acttgcgcac
     1561 tccagttgaa gagttctttt gcacgcgaga cgtcaagccc gcggcgaggt tgcccattgg
     1621 gtttgtctgt ctgccagacg attttgcctt ggaagttggt aa
//
LOCUS       DAOREF010000767         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_65464_length_1659_cov_3.241272, whole genome shotgun sequence.
ACCESSION   DAOREF010000767 DAOREF010000000
VERSION     DAOREF010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65464_length_1659_cov_3.241272"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..915)
                     /locus_tag="PKI33_14040"
     CDS             complement(10..915)
                     /locus_tag="PKI33_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38185.1"
                     /translation="MSLPVIYVENLTRRFGDFVAVDHINFEVNEGEVVGYLGPNGSGK
                     TTTIRMLLGLLAPSEGEATVLGYDVHRQSEEVRARVGYMSQKFAIYDDLTTLENLTFY
                     GGVYGITDKARIMETLELVGLSGHEHTLTRDLSTGWRQRLSLGIALVHKPKLLFLDEP
                     TSGVDPTARRAFWDLIYQLAESGVTILVTTHYMDEAEYCERVGVMRDGKLLAMDTPTN
                     LKKSIITGDVWEVFAHPLEDGLDALSDVDGIERVGLAGDHLRVISSNVKEEALQGLLS
                     KKKIRVERIEKGEPTLEDVFLALAR"
     gene            complement(916..1350)
                     /locus_tag="PKI33_14045"
     CDS             complement(916..1350)
                     /locus_tag="PKI33_14045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNM38186.1"
                     /translation="MINIDLGVLIDRPVKDVFAFVSNPNNMTKWNSAVINLEPLTPGT
                     VGVGAKFKTTGEAMGRRIEGEMQITAYEPDTKCGFQVTAGPMQVNLTMIFKTVGTGTK
                     VSLNAQGNPGGLFKLAEGAMAGQVKSMMEGNLSRLKSALEGS"
     gene            complement(1366..>1659)
                     /locus_tag="PKI33_14050"
     CDS             complement(1366..>1659)
                     /locus_tag="PKI33_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HNM38187.1"
                     /translation="SNLRSLLNGRILELRGSPLNTLRHVAHTDVDVEDVQAFGDRLHI
                     RVRDDKANQVLRRLKSSIKAAGGHVVELRMVPPALEDVFIALSEDKAISAVSV"
BASE COUNT          431 a          454 c          360 g          414 t
ORIGIN      
        1 cctacatctt taacgagcca acgctaaaaa gacatcctcc agagttggct ctcctttttc
       61 gattcgttca acccgaatct tcttcttact taataaacct tgcaacgcct cttctttgac
      121 gtttgagctg ataacccgca aatgatcgcc agccaacccg acgcgttcaa tgccatctac
      181 gtcacttagt gcgtccagcc catcttcgag cggatgtgca aatacctccc agacatcacc
      241 cgtaataata gattttttca aattcgtcgg ggtatccatt gccagcaatt tgccatcacg
      301 catcacacct acacgttcgc agtattcggc ttcgtccatg taatgtgtag tgactagaat
      361 ggtcactcca ctttcagcta gttgatagat caaatcccaa aatgcgcggc gggcagtagg
      421 gtccacacca gaggtgggtt catcaagaaa gagcaacttc ggtttgtgga caagcgcaat
      481 gcccaaggat agccgttgac gccagccggt ggacagatcc cttgtcagcg tatgctcatg
      541 cccagagaga ccgaccagtt ccaacgtctc catgatgcgg gctttgtcgg taatcccata
      601 cacgccaccg taaaaggtca aattctccag cgtggttagg tcatcataaa tcgcaaactt
      661 ctgcgacata tacccaaccc gtgcgcggac ttcttcactt tggcggtgga catcgtaccc
      721 caatactgtt gcttcgcctt cgctaggggc aagcaaacca agcaacatgc gaatggtagt
      781 ggttttaccc gagccattgg gtccaagata accaaccact tcaccttcgt tcacttcaaa
      841 gttgatatga tccaccgcga caaaatcgcc aaaacgccgg gtgagatttt caacataaat
      901 aacaggcagt gacatttacg atccttccaa tgcagatttg aggcgagaaa gattgccttc
      961 catcatagac ttgacctgcc ccgccatcgc gccttcagcc agtttgaata aaccgccggg
     1021 gtttccttgc gcattcaaac taacttttgt acctgtacct actgttttga agatcatggt
     1081 caagttcact tgcatgggtc cggcagtgac ttgaaagccg cacttggtat cgggttcata
     1141 ggctgtaatt tgcatttcac cttcgatgcg ccgccccatg gcttcgccgg tcgttttgaa
     1201 cttcgcacct acaccaactg tgcccggcgt gagcggctct aaattgatga cagctgaatt
     1261 ccattttgtc atgttgttcg ggttggaaac aaaagcaaaa acatctttga cagggcggtc
     1321 gatcaataca cctaaatcga tgttaatcat ggcaggctcc ttttattaaa ccgatacagc
     1381 agagatggct ttatcttcag ataaagcgat aaacacatct tccaaggcgg ggggcaccat
     1441 acggagctca accacatgcc cgcccgcagc tttgatactg gacttcaagc ggcgcaatac
     1501 ttgattcgcc ttatcatcac gcacacggat gtgaagccgg tcaccaaagg cttgcacatc
     1561 ttccacatcc acatcggtat gggcaacatg ccgcagagta ttcaaaggtg agccgcgcaa
     1621 ttcaaggata cgtccgttta gaagtgaacg caaattaga
//
LOCUS       DAOREF010000768         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_65465_length_1659_cov_3.226309, whole genome shotgun sequence.
ACCESSION   DAOREF010000768 DAOREF010000000
VERSION     DAOREF010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65465_length_1659_cov_3.226309"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PKI33_14055"
     CDS             <1..737
                     /locus_tag="PKI33_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNM38188.1"
                     /translation="WASNNKFALIGGFRQVAVMVSFEVPMLTMLLIPVIFSGSMNMNA
                     IIQAQDPIPFWVLSPLGALIFLIAAIAELGRAPFDLAEGESELVSGFNIEYSGMKFGM
                     FYAGELLHAFTFGGFWAILFFGGYRFFGLEQVSPFLAILILIIKAMLGYWVIMWVKYT
                     MMRIRIDQMLSFNWKFLTPLSFALLLVTALMNALLASASTWLYVLGMILANIVTGWIA
                     LEIARSATRSERERIEGVKQPVEAHH"
     gene            798..1322
                     /locus_tag="PKI33_14060"
     CDS             798..1322
                     /locus_tag="PKI33_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNM38189.1"
                     /translation="MTAQQIIFLIVALYTLISATMVVTSRNLVHAALWLVSTLFGVAV
                     VYALLNANFIAVVQVVVYIGAIAILFIFAVMLTRKDMRDTGPQMNKSWWAGALVAVLT
                     FAGLVSLLSGWTGFTKTAAQLPAGFDSISLLGEALVSPNAFVLPFEVASVLLLAALVG
                     AVYVAFNPRSGDPK"
     gene            1337..1648
                     /gene="nuoK"
                     /locus_tag="PKI33_14065"
     CDS             1337..1648
                     /gene="nuoK"
                     /locus_tag="PKI33_14065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNM38190.1"
                     /translation="MVPLSWYLFLSAALFSIGLFGVLVRRNAIAILLGIELMLNAVNI
                     NLVSFWRYSDALTSMNGQVFSIIVFAVAAAEVSVGLALVISVYRRRNTVVADELDMLK
                     W"
BASE COUNT          310 a          394 c          430 g          525 t
ORIGIN      
        1 ggtgggcatc aaataacaaa ttcgccttga tcggtggttt ccgtcaggtg gcggtgatgg
       61 tctcattcga agttccaatg ttgaccatgc tgttgatccc agtaatattt tcgggatcaa
      121 tgaacatgaa tgccattatt caagcacaag accctattcc attttgggtt ttgtctccgc
      181 ttggggcgtt gatcttcctg attgctgcca ttgccgaact gggacgcgct cctttcgacc
      241 ttgctgaagg tgaatccgaa ttggtctctg gcttcaacat cgaatactca ggtatgaagt
      301 tcggtatgtt ctatgctggt gaacttctgc atgcctttac ctttggtggt ttctgggcaa
      361 tccttttctt tggcgggtat cgcttcttcg gtttggaaca ggtcagcccc ttccttgcca
      421 tccttatcct catcatcaaa gccatgctgg gatattgggt catcatgtgg gttaagtaca
      481 ccatgatgcg tatccgtatc gaccagatgc tttcctttaa ttggaaattc cttacgccct
      541 tatccttcgc cttgttactg gtaactgcgt tgatgaatgc gcttcttgcg tccgcttcta
      601 cttggctcta cgtcctcggc atgatccttg cgaatatagt taccggctgg atcgcgcttg
      661 agatcgcacg ctctgccacc cgtagcgaac gcgagcgcat cgaaggcgta aaacaacctg
      721 tggaggctca tcactaggag acggcatttt acaaattacg tactccgtga tttgtaaaga
      781 ccggaaatgg atattctatg acagcacaac agattatttt cctcattgtg gctctttaca
      841 ccctcatctc tgcgacgatg gtggtgacca gccgcaacct tgttcacgct gcattgtggc
      901 ttgtctccac attatttggt gtggcggtgg tctatgcgct tctgaatgcg aacttcatcg
      961 ccgtggtaca ggttgtcgtt tacatcggcg ccatcgccat tcttttcatc ttcgctgtga
     1021 tgcttactcg taaagatatg cgcgatacgg gtccgcagat gaataagagt tggtgggcag
     1081 gcgctttggt ggctgtactc acatttgctg gtttggtttc cctgctctca gggtggaccg
     1141 gcttcacaaa aacagctgcc cagctcccgg cggggtttga ttcgatcagt ctgctcggcg
     1201 aggcgttggt ttcgccgaat gcgtttgtct tgccgtttga agtggcgtct gttctattat
     1261 tggcagcgtt ggtgggtgcg gtgtatgtcg cattcaatcc tcgttctggc gaccctaaat
     1321 aggaggcgga taaataatgg ttcctctttc ctggtattta ttcctttcgg cggcattgtt
     1381 tagcattggc ttgtttggtg tgctggtgcg acgcaatgcg atcgccatcc ttctcggtat
     1441 tgagttgatg ctcaatgctg tgaacatcaa cctcgtttct ttctggcgct atagcgatgc
     1501 cttgacctcg atgaatggac aagtcttttc cattatcgtc tttgcagttg cggcggcaga
     1561 ggtttcggtg ggcttggctt tggttatttc tgtctatcgc cgtcgcaata cggtggttgc
     1621 agatgaactc gatatgttga agtggtaggg acgcatgac
//
LOCUS       DAOREF010000769         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_65783_length_1655_cov_3.385625, whole genome shotgun sequence.
ACCESSION   DAOREF010000769 DAOREF010000000
VERSION     DAOREF010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65783_length_1655_cov_3.385625"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKI33_14070"
     CDS             <1..247
                     /locus_tag="PKI33_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38191.1"
                     /translation="NVSGEIFDGNGNPYESFNGTMEWGKGWRANGTYKERGDISLWIN
                     ASETGFEFVNPKGNIQQLCAVREGVASAYLGSYSCEP"
     gene            329..619
                     /locus_tag="PKI33_14075"
     CDS             329..619
                     /locus_tag="PKI33_14075"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNM38192.1"
                     /translation="MKLPELKAIKLAELFSSLSDASRIRIIASLLDGEMSVRVLAEGL
                     GMTESAVSHQLRGLRQMHLVRARKSGRQVFYSIEDDHVARLFTLGLDHVQHG"
     gene            623..1561
                     /locus_tag="PKI33_14080"
     CDS             623..1561
                     /locus_tag="PKI33_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNM38193.1"
                     /translation="MHTHSHTHIGDLTQQTTKRLTLSLVLTAAFVIVEIVAGVFGNSL
                     ALLTDAAHNFTDVIALGLSWYALRIAMKPAHAGKTFGYHRVGILVALINSTTLILIAF
                     GIFFEAYHRFLTPPEVDSTILIGVGAVAFIINLVTALMVKEGSEHDLNMRSAFLHLMG
                     DVMSTLGAVIAGIIIAFTKWNWLDPLVSVLIGAFILYNAWGILKQTIHILLESTPENI
                     NVDNMVLDLQNINGVLGVHDLHVWSISENLRMLSAHVVIGDTTIGEGVSIQQNINELL
                     AHSYNIHHATLQMECEGCTSKDLFCQITEAHQHAHH"
BASE COUNT          427 a          428 c          352 g          448 t
ORIGIN      
        1 aaatgtgagt ggtgaaatct ttgacggcaa tggcaacccc tacgaaagtt tcaatggcac
       61 aatggaatgg ggtaaaggct ggcgcgccaa tggcacctac aaggaacgcg gcgatatttc
      121 gctatggata aatgcctcag aaaccggctt cgaatttgta aacccgaaag gtaacattca
      181 acaactttgt gcagtgcgtg aaggcgtagc aagtgcatat ctaggcagtt attcctgcga
      241 accgtaatta ctcagaccct gccaaacacc atattgacag ggtctttttt acagagtaat
      301 atatgaatag ttgttcatat attcaattat gaagttacct gaactcaaag ccatcaaact
      361 tgccgaacta ttcagttccc taagcgatgc aagtcgcatt cgcatcatcg catccctctt
      421 ggatggagaa atgagcgtcc gcgtgcttgc tgaaggactt ggcatgaccg aatccgctgt
      481 atcgcatcag ctccgtgggt tgcgccaaat gcatctcgtt cgcgcacgca aatctggcag
      541 acaagttttt tattccatcg aagatgacca cgtagcgcgg ctatttacgc tgggtctcga
      601 tcacgttcag cacggataac ccatgcacac tcattctcat acccatattg gcgatcttac
      661 ccagcaaacc accaaacgcc tcaccctctc cttagttctc actgcagcat ttgtcattgt
      721 tgagatcgtc gcaggtgttt ttggcaacag tcttgctttg cttaccgatg cagcccataa
      781 cttcaccgat gtcattgcat taggcttaag ttggtatgcc ttacgcatcg ccatgaaacc
      841 cgctcacgct ggcaagacct ttggctatca ccgtgttggg attcttgtcg cgcttattaa
      901 ctcgacgacg ctaattctta tcgcctttgg gattttcttc gaagcctatc accgcttctt
      961 gactccgccc gaagttgatt cgactatcct gatcggagtt ggcgctgtgg cttttatcat
     1021 caacctcgtc accgcattaa tggtcaaaga aggcagcgaa catgatctca acatgcgcag
     1081 cgcctttctt catctcatgg gtgatgtcat gtccacactt ggggcggtca ttgcaggtat
     1141 catcatcgcc ttcacaaaat ggaactggct cgacccgctt gttagtgttc ttatcggtgc
     1201 ctttatctta tataacgctt ggggcatcct gaagcagacc attcacatct tgctagaaag
     1261 cactcccgaa aacataaatg tagataacat ggtgcttgac ctgcaaaaca tcaacggtgt
     1321 tttaggcgtc catgacctgc atgtgtggag catcagtgaa aacttgcgca tgctctcagc
     1381 gcatgtggtt atcggcgata ctaccattgg cgaaggcgtc tctattcaac aaaacattaa
     1441 tgaactgctt gcccacagtt acaatatcca tcacgccact ttacaaatgg agtgtgaagg
     1501 ctgtacatcc aaagacttgt tttgccagat cactgaggca catcaacacg cccaccacta
     1561 acttttattt ggagtcccat gtctcaagcc gcaaaagcac aacaactttt atccttacac
     1621 aacaacggta aactgctcat tctgcctaat gtgtg
//
LOCUS       DAOREF010000770         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_65983_length_1652_cov_6.314966, whole genome shotgun sequence.
ACCESSION   DAOREF010000770 DAOREF010000000
VERSION     DAOREF010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65983_length_1652_cov_6.314966"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKI33_14085"
     CDS             complement(<1..533)
                     /locus_tag="PKI33_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38194.1"
                     /translation="MNNIWTIAKREYNNYFNGPLAYVVMLAILLPLGIYFSLILFYSS
                     QQGLYGGSAAPDITPVNGLFAFLMIFLSPALTMRLLSDEARMGTLELLLTAPVRDIEL
                     VVGKWLGAMLFILTLLGTTLIYPLVLNNFVDPGIDWMVVLSSYIALILLCGTMLALGV
                     GISAIFTNQFAAFFVTLG"
     gene            complement(538..1461)
                     /locus_tag="PKI33_14090"
     CDS             complement(538..1461)
                     /locus_tag="PKI33_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM38195.1"
                     /translation="MIKVSGLTKDYGSRRAIHNLSFDAQQGEIVGFLGPNGAGKTTTM
                     RILTGYMPPTDGEAIVAGYDVVEESLEVRKRVGYLPESVPLYYDMVVSEYLKYMGELR
                     RIPNVDDRVDEVLDMVGLFDRADGYIGNLSKGMKQRVGLAQALLHRPEVLILDEPTIG
                     LDPGQVVEVRELIREIGKERTVLLSTHLLNEAQNLCDRVLIINKGKIVAEDTTENLQA
                     RLLGAERVVIRVRGEAEELADSIKSIKGVRKVETKDDGAVEFEFASGKDLRPEVAKQV
                     IKDGYDLLELRPLGMSLEEIFLELTGNEGKK"
BASE COUNT          435 a          431 c          413 g          373 t
ORIGIN      
        1 ccgagggtca cgaagaaggc agcgaactgg ttcgtgaaaa tggcagagat gccaaccccc
       61 aaagccagca tggtaccgca aagcaggatc aaagcgatat aagaagaaag caccaccatc
      121 cagtcgatgc cggggtcaac gaagttgttc aacacgagcg gatagatcaa cgtagtgcca
      181 agcaaggtga ggatgaacaa catggcgccc agccacttgc caaccaccag ctcaatatcg
      241 cggacggggg cggtcagcaa aagttcgaga gttcccatac gagcttcgtc agagagcaaa
      301 cgcatagtaa gcgccgggct gaggaagatc ataaggaaag caaacaaccc gttgacggga
      361 gtgatatcgg gggcagcgct gccgccatat aaaccttgct ggctgctgta gaaaaggatc
      421 aaactgaagt aaatgccaag tggcaaaagg atcgccagca tcacaacata ggcgagcggt
      481 ccgttaaagt agttgttgta ttcgcgtttt gcgatggtcc aaatattgtt catgggatta
      541 tttcttccct tcgtttccag tgagttcgag gaagatttct tcgaggctca tgcccaacgg
      601 gcggagttcc aaaaggtcgt acccatcttt aataacctgt ttggcaactt cggggcgtaa
      661 atccttgccg gaggcaaact cgaattccac agcaccgtcg tctttggttt ccaccttgcg
      721 gacaccctta atgctcttga tcgagtctgc aagttcttcg gcttcgccac gtacacggat
      781 cacaacccgt tcggcaccga gcaagcgagc ctgcaagttt tcggtggtgt cttcggcgac
      841 gatcttgccc ttgttgatga tcaagacgcg gtcgcagagg ttttgcgctt cattcaaaag
      901 gtgagtggag aggagcaccg tgcgctcttt accaatctcg cggattaatt cgcggacttc
      961 taccacctgc ccagggtcga gaccaattgt cggctcatcg aggatgagca cttcggggcg
     1021 gtgcaaaagc gcctgagcca agcccacacg ctgtttcata cctttggaca ggttgccaat
     1081 gtacccgtcg gcacggtcaa acaagccgac catatccaac acttcatcca cacggtcatc
     1141 cacattcggg atgcggcgaa gttcgcccat atactttaag tattcagaaa cgaccatgtc
     1201 gtagtacagc ggcacagact ccggcaaata gccgacgcgc ttgcgtactt ccaacgattc
     1261 ttccaccaca tcgtaaccag ctacgatcgc ttcgccatca gtaggcggca tgtaaccagt
     1321 caggatgcgc atggtggtgg ttttcccggc gccattggga cccaaaaagc ccacaatctc
     1381 gccctgttgg gcatcaaaac tcagattgtg gatggcgcga cgtgacccat agtcttttgt
     1441 gagaccacta actttgatca ttgcttctcc tcacggtgaa ataatatata aaatgggatt
     1501 atcgcaaaga aaagacacag ccgtgttggc ggtgcctttt ttttcgtacc gcagaatact
     1561 atacaatata tgaaagacca aagtcaagcg ggtgtatagc cctctaatcc aatcctaatt
     1621 ttgtctgacc aattcattcc atcttgtata at
//
LOCUS       DAOREF010000771         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_66230_length_1649_cov_3.001255, whole genome shotgun sequence.
ACCESSION   DAOREF010000771 DAOREF010000000
VERSION     DAOREF010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66230_length_1649_cov_3.001255"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PKI33_14095"
     CDS             <1..1327
                     /locus_tag="PKI33_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38196.1"
                     /translation="VAPPETVPGSNLVPGGESTQVRMVAETVTLTISQDPADEKRAMA
                     ETRAVFTMRNLGTAEESMAVRFPLSFFDGASDGFFNYPELSSIAVKVDGKTVPTRREM
                     QPFLASQSNSSDRDEIPWSVFDVTFPPNQDVTIEVVYNVDGFGDYPYEVFKYVLETGA
                     GWNGTIGSAEIILRLPYEANTYNVWTEGVEGYGDATPGGAFSGNEVHWKYTELEPTFE
                     NNLQFVIVTPSAWGKVLKEDENVTQNPEDGEAWGRLAKAYKELILMRHGNLRTDPAGQ
                     SMFELSRSAYETCLGILPNDPLWLYGSAELLWAHYYFDFYMMGQPDTQNLLATTLTRL
                     NSALEIDPNLQVAKDKLEEISYSIPEAVQKNEDGSFALIGLTATPAPLVWKVNVTPTI
                     PPVPTPTATFTMGMIPPNTPEPTASNPICGGAFLLPALFGLVLSVKRKR"
     gene            complement(1366..>1649)
                     /locus_tag="PKI33_14100"
     CDS             complement(1366..>1649)
                     /locus_tag="PKI33_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38197.1"
                     /translation="EEKFYKPASAAILQTQMDDLRMGKKVSIYGIGIDPSGLSYGLKS
                     ILWTDIEALKAEKDKLLIKQNGEWNVWETFAFNIPEEVLVLSQVFTKVE"
BASE COUNT          419 a          397 c          390 g          443 t
ORIGIN      
        1 cgtggctccc ccggagactg tccctggctc gaaccttgtg cccggcggcg aatcaaccca
       61 agtccgcatg gtagcagaga cggtcacact cactatctct caagacccgg cggacgaaaa
      121 aagagctatg gctgaaacac gagccgtttt caccatgcgc aatttaggaa cggcagaaga
      181 gagcatggca gtgcgttttc cgctttcttt tttcgatggt gcatcggatg gctttttcaa
      241 ctaccctgaa ctttcctcca ttgccgtaaa ggtggatggg aaaactgtgc caactcggcg
      301 tgagatgcaa ccctttttag ccagccaatc aaactcttca gaccgcgatg aaattccttg
      361 gtctgttttc gatgtgacct ttccgcccaa tcaagatgtc actatcgagg ttgtatacaa
      421 tgtggatggc tttggcgatt atccttatga agtcttcaaa tatgttcttg aaacgggcgc
      481 cggttggaat ggcactattg gcagtgccga gatcattctg cgccttccct atgaagcaaa
      541 tacctacaat gtatggacag aaggcgtgga aggctatggc gatgccacac ccggtggtgc
      601 gtttagcggg aatgaagtcc attggaagta caccgaatta gagccgacct ttgaaaacaa
      661 tcttcaattc gtgattgtga ctccatctgc ttggggaaag gtgctgaaag aagatgagaa
      721 cgtcacccag aatccagaag atggtgaagc ttggggcagg cttgcaaaag catataaaga
      781 acttattcta atgaggcatg gcaacttgcg tacagaccct gcagggcaga gcatgttcga
      841 gttaagccgt agcgcctatg aaacctgtct tggtatcttg ccgaatgatc cactttggct
      901 atacgggtct gctgagttgt tgtgggcgca ttattacttc gacttttata tgatggggca
      961 gcccgatact caaaacctct tagcaacgac gttgacacgc ctcaattcag ccttagagat
     1021 tgaccccaat ctgcaagtgg cgaaagacaa acttgaagaa atcagctact ctatcccaga
     1081 ggcggttcag aaaaatgaag acgggtcttt tgctttgatt ggtctcaccg ctacaccagc
     1141 tccgcttgtg tggaaggtta atgtcacccc aaccataccg cctgtcccta cacctacggc
     1201 tacattcacc atgggcatga ttcctcccaa cacccctgag cctacggctt ccaatcctat
     1261 ttgtggggga gcatttctcc tgccggcatt atttgggttg gtattgagtg taaaacgtaa
     1321 acgctagcca acagaaaagt cactccatat ggagtgactt ttctgttatt ctactttcgt
     1381 aaatacttgt gagagcacta gcacttcttc tggaatattg aaggcgaagg tttcccatac
     1441 gttccattcg ccgttttgtt tgataagcag tttgtctttc tctgctttaa gggcttcgat
     1501 atctgtccac agaatagact tgagtccata gctcaaacca gatgggtcaa ttccaatccc
     1561 ataaatggaa acttttttgc ccatccgtag atcgtccatt tgagtttgta aaatggcagc
     1621 agaagcaggc ttgtagaact tttcttcaa
//
LOCUS       DAOREF010000772         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_66298_length_1648_cov_4.355932, whole genome shotgun sequence.
ACCESSION   DAOREF010000772 DAOREF010000000
VERSION     DAOREF010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66298_length_1648_cov_4.355932"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..822
                     /locus_tag="PKI33_14105"
     CDS             184..822
                     /locus_tag="PKI33_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38198.1"
                     /translation="MKKNLYSFIFLLLVGALLSACSLPSAAETQSAQPVNDPNAVIPQ
                     SVATASVVTTSSLPAGMPTVETDLSKILGVLQSGNFSYLANLASEKYDASAYTVPGSL
                     VFNAAFPSSQTIYLNYGWCAKDLATLQQNLQHISTYFSFNGQQIPMEYVNTISTESSD
                     GLQCSNTGLLLSGWAPGTYQFQVKASFDSQINDGSADYPAGDYISEYTVTVQ"
     gene            965..>1648
                     /locus_tag="PKI33_14110"
     CDS             965..>1648
                     /locus_tag="PKI33_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM38199.1"
                     /translation="MTLISNPSTLKHLEGKEQLLLKLVELSVTLNSTLDLEELLQLIT
                     ATATEALNCEAASILLYDEKHPRLYFAAATGSNPAKLAEIPVPIDSSLAGTIFRTNQP
                     MILNNADQDPRHFPRVAAHINLKVKTLLGVPMPIKDQTMGVLEAVNKRDGSFDESDAA
                     ILSVVAAHAAIAINNARLFRARQQALEKVRAMNEVKNNFLALASHELRTPLGIIIGYA
                     TFLQNDSTGE"
BASE COUNT          452 a          464 c          327 g          405 t
ORIGIN      
        1 ggtccgaatg gacaaaacgg tcagatgccc ggcggtcaga acggtcagca gcccagtcaa
       61 ggtcaaggtg gtccgaacgg aacacctccc aatggtcagg ctcctggcac acgtccgtaa
      121 ctctttataa aaatgtcagc ccgcatgtac ggtgcgggct gacacctccc ataaaaaatt
      181 cctatgaaaa aaaatcttta ttcctttatc tttcttcttc ttgttggggc acttctaagc
      241 gcttgctctc ttccttctgc cgctgaaact caatctgctc aacccgtcaa tgaccccaat
      301 gctgtaatcc cacaaagtgt ggcaaccgcc tctgtcgtaa caacctcatc cctacccgcc
      361 ggcatgccaa ctgttgaaac agaccttagc aaaatactag gtgttttaca gagtgggaac
      421 ttctcttatc ttgcaaatct cgcttcagaa aaatatgatg ccagcgcata tacggttccc
      481 ggctccctag tcttcaatgc agcctttccc agcagccaaa ccatttatct aaattatggc
      541 tggtgcgcaa aagacctcgc aaccttacaa cagaaccttc aacatatttc cacgtacttc
      601 tcttttaatg gtcaacaaat ccccatggaa tatgtgaaca ccatcagcac agaaagcagc
      661 gacgggttgc aatgttccaa tacgggttta ctgctttcag ggtgggcacc cggcacctac
      721 caattccaag taaaagccag ctttgattca cagatcaacg atggcagtgc cgattacccc
      781 gcaggagact acatttcaga atatacggtc accgttcaat aaaccacact cacccccgaa
      841 gacagccttt cgcgggtgag ttttcattta attacagttt tattagccct aattggtacg
      901 aatggttggt tctcaataca caggaggaat ctataattca gaatgaaagt caagccaaaa
      961 cctcatgacc cttatctcaa accccagcac gcttaaacat ttggaaggca aagaacaact
     1021 tctcttgaag ctggttgagt tgagcgttac cctcaactca actcttgatc ttgaagaatt
     1081 gctacaactc atcaccgcta ccgctaccga agccctgaac tgtgaagccg catccatcct
     1141 gctttacgac gaaaaacatc cgcgcttgta ttttgctgcc gccactggct caaaccctgc
     1201 caaactggca gagattccgg tgccaatcga tagcagcctc gccggaacaa tcttccgcac
     1261 caaccaacct atgatcttga acaatgcaga tcaagacccg cgtcactttc cgcgcgtggc
     1321 agctcatatc aacctcaagg tcaagacctt gttgggagtg cccatgccca ttaaagatca
     1381 aaccatgggc gtgcttgaag cagtcaacaa aagagacggc agcttcgacg aaagtgatgc
     1441 tgctattttg tctgttgtcg ccgcacatgc tgccattgcc attaacaatg ctcgtctctt
     1501 cagagcacgc caacaagcct tggaaaaagt ccgcgcgatg aacgaggtca agaataattt
     1561 tcttgctttg gcttcccacg agttgcgcac tcctttgggc atcattattg gctatgccac
     1621 ctttttacaa aatgattcaa ccggcgaa
//
LOCUS       DAOREF010000773         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_66299_length_1648_cov_4.280603, whole genome shotgun sequence.
ACCESSION   DAOREF010000773 DAOREF010000000
VERSION     DAOREF010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66299_length_1648_cov_4.280603"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKI33_14115"
     CDS             <1..255
                     /locus_tag="PKI33_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38200.1"
                     /translation="GMTTAVNIITEEVKNTLLIPNRAVRLVNGERVVYLEKDGQATPV
                     TITLGASSDTMSVLTSGDIKEGDLIILNPPSANGGPFGGG"
     gene            257..955
                     /locus_tag="PKI33_14120"
     CDS             257..955
                     /locus_tag="PKI33_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38201.1"
                     /translation="MDWVIEARDLKKTYVMGEIEVQALRGVSFTVTRGEVVSIMGPSG
                     SGKSTMMNTLGCLDRPSSGEYILDGESVAQMNDDQLATVRNRKIGFVFQSFNLLSRLS
                     AVGNVELPLRYAGITQGRREKAQAALEAVGLKDRMFHRPYELSGGQQQRVAVARALVN
                     NPAIIMADEPTGNLDSKVGKEIMTLLLNLNKDLGTTLVIVTHDPKIAEQTQRVIRLKD
                     GELDSIDINSGGAQ"
     gene            952..>1648
                     /locus_tag="PKI33_14125"
     CDS             952..>1648
                     /locus_tag="PKI33_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38202.1"
                     /translation="MSFAQAIMEALESLNGNKMRSGLTVLGIVIGVAAVIAMLAVGNG
                     AQASITGSISSIGTNLLFIFGGAPSQDGPPGSGPRTSGNNERPLTLGDSNALLDTFAA
                     PSVEMVAPAIQGNALLTFSSEKTSTSISGVTPEYSQVRNLEIAEGEFINQEHILGRMS
                     VVVLGPETAIDLFGHADGVVGETIRIEGQPFRIIGVLVARGGGAFGSEDNSAYIPFTT
                     AQARLIKRGARDEV"
BASE COUNT          407 a          405 c          414 g          422 t
ORIGIN      
        1 ggtatgacca ccgctgtaaa catcatcact gaagaagtga aaaatacgct tctcattccg
       61 aaccgcgctg tccgtttagt gaatggcgaa cgcgtggtct atcttgaaaa agatgggcaa
      121 gcgacacccg ttaccatcac cttaggcgct tcctcagaca caatgagtgt actcaccagc
      181 ggtgatatta aagagggtga tttgatcatc ctcaacccgc cctcagccaa tggaggtccc
      241 ttcggtggag gctagcatgg actgggtcat tgaagcacgc gatttaaaaa agacctacgt
      301 catgggtgag atcgaagtgc aagccttacg cggagtttca tttactgtga ctcgcggaga
      361 ggtggtttcc atcatgggtc cttccggctc cggtaaatcg accatgatga acactcttgg
      421 ctgtttagat cgcccatcat caggtgaata cattttggac ggcgagtctg ttgcgcagat
      481 gaatgacgat caattggcaa cggttcgtaa tcgcaaaatt ggctttgtct ttcaaagctt
      541 taatctgctt tcgcgcctct ctgctgtcgg aaatgtagaa ctgcctttac gctatgccgg
      601 catcacacaa ggacgccgcg aaaaggcaca agctgctctc gaagccgtgg gtcttaaaga
      661 tcgtatgttc caccgcccct atgaactttc gggcggtcag caacagcgtg tagcagttgc
      721 ccgcgcactc gtcaacaacc ctgccatcat catggcagat gagccgactg gtaacttgga
      781 ttcaaaagtc ggtaaagaga ttatgactct tttgcttaac ttgaataaag atctcggaac
      841 cacccttgtc atcgtcacgc atgacccgaa gatcgccgag caaactcagc gcgtaatccg
      901 cttaaaagat ggcgaattag attccatcga tataaactcc ggaggcgcac aatgagtttt
      961 gcacaagcaa tcatggaagc cctcgaaagc ctgaatggca acaaaatgcg ttcagggctt
     1021 actgtgttgg gaattgtcat cggtgtggca gcagtgatcg ccatgttagc tgttggaaat
     1081 ggcgcacaag cgtccatcac cggttcaatt tctagcattg gtacgaactt gctcttcatt
     1141 ttcggtggcg cgcctagcca agatggaccg cctgggtcag gtccacgcac cagtggaaat
     1201 aatgagcgtc cattgacctt gggcgactct aatgcattgt tagatacctt tgcggctccc
     1261 tctgtggaaa tggtagcacc tgcgattcaa ggcaatgcct tgctcacgtt ctctagtgaa
     1321 aaaacaagca cctctatttc tggggttact cctgaatatt cacaagtccg taacttggag
     1381 atcgctgaag gcgaatttat caatcaagaa cacattttgg gacgcatgtc cgttgttgta
     1441 cttggacctg aaaccgccat tgacctattc ggtcatgccg atggtgttgt aggtgagacc
     1501 attcgtattg aaggtcagcc cttccgcatt attggcgttt tggtggcacg tggcggtggt
     1561 gcttttggta gtgaagataa cagtgcctat attccgttta ctactgctca agctcgcctc
     1621 atcaaacgcg gcgctcgcga tgaagtgg
//
LOCUS       DAOREF010000774         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_66315_length_1648_cov_3.099184, whole genome shotgun sequence.
ACCESSION   DAOREF010000774 DAOREF010000000
VERSION     DAOREF010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66315_length_1648_cov_3.099184"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..>1648
                     /locus_tag="PKI33_14130"
     CDS             383..>1648
                     /locus_tag="PKI33_14130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNM38203.1"
                     /translation="MLYFFNRFIEILRDIPRWLRPGLGVKRWLLFVMAGITLIGLGIA
                     VVLIDIYRTNTTDPVVLTFLSYASLRFLPRLLRAIVFGGLGVALIVYGMYKINRSLLR
                     PFLQPGSDIISTLAAFQRLNRGPHIVTIGGGHGLSTLLRGLKTHTRNLTAVVTVADDG
                     GSSGKLRETFGILPPGDIRNCLAALSNDEAMLTQLFQYRFSGAPGLEGHSFGNLFITA
                     LADITGSFESAVVESGRVLSVNGRVLPSTLHNVQLVASLELPHTLNEVRVEGESKIPE
                     MAGRVRRVWLEPDDAPAFPPVIKELLTADIVVVGPGSLYTSLLPNLLVHDLLDSLHAS
                     RAVKIYVCNIATQSGETDAFTCYDHVRALEGHVGENLFDIILCNDNYKGDLGTSSVWV
                     RADEKTLSDERTYTADLVDDAHPWRHDSTK"
BASE COUNT          420 a          326 c          381 g          521 t
ORIGIN      
        1 tttggaccgg gaagatttat atttgccata actactcctt ggaatgaatt gaaaaaccag
       61 attttgttta aactggcatc tgtatgccag gtaaatctgc attagcatcg aattgagctt
      121 ccgtaattat caatgcaact aaacaatata agttgatatt gcatctaaac cgataaaaaa
      181 gcatcttaca aagaaactaa gataggggaa gtagtgacta ttgagaaaat aaatcttgcg
      241 tatagacaac gctggtattt acttgccact gtcataactg gcaaaaattg atgacatact
      301 gtgattatac cgtaaggcgg tttcccttta tttttggcaa caggtttgac gtcaaaggtc
      361 aatcctgcta ttatcgattc ttatgcttta cttttttaat cgttttatag aaattctacg
      421 cgatattcca cgctggcttc gaccagggtt aggggttaaa cgctggcttt tattcgtaat
      481 ggctggtatc acgttgattg gcttaggtat tgctgttgtt ttaattgata tctaccgcac
      541 aaatactaca gaccctgttg tgctcacgtt tttatcctat gcttccttgc ggtttttacc
      601 tcgtctttta cgtgccatcg tttttggtgg acttggcgtg gcattaattg tttatgggat
      661 gtataagatt aaccgctctc ttctccgccc atttctgcaa cccggttcag atatcattag
      721 cacgctggca gcctttcagc gtctcaaccg cggacctcat atcgttacta ttggtggtgg
      781 acatggactt tccactttat tgcgtggatt aaaaacacac acacgaaact tgactgccgt
      841 tgtgactgtt gcagatgacg gtgggtcgtc tggcaaattg cgtgaaacct ttgggattct
      901 tccacctggt gatatacgca actgtcttgc tgccttatct aatgatgaag caatgttgac
      961 acagcttttt caatatcgct ttagcggggc accggggttg gagggacact cttttgggaa
     1021 cctatttatc acagcgttgg cagatattac gggaagtttt gaatcagctg tggtcgagtc
     1081 tggacgcgtt ctttcagtca atgggcgtgt attgccatcg acattacata atgttcaatt
     1141 ggttgcaagc cttgaattac cgcacacttt gaatgaagtt cgagtagaag gggagagcaa
     1201 gattccggaa atggctggga gggttcgccg cgtctggctt gagccggatg atgcgccagc
     1261 atttcctcct gtgatcaaag aactactgac agcagatatt gtggttgtgg gaccgggaag
     1321 tttatataca agtttgctcc ccaatctctt agttcatgat ctgttagata gtttgcatgc
     1381 cagccgtgcc gttaagatct atgtgtgtaa tattgctaca caatctggtg aaacagacgc
     1441 ctttacttgc tatgatcatg tccgtgcttt ggaagggcat gtgggtgaaa acttatttga
     1501 tattattttg tgcaatgata attacaaagg cgatttgggg acttcatcag tttgggtgcg
     1561 agcagatgaa aagacgcttt cagatgaaag aacctatact gccgatctag tggacgatgc
     1621 tcatccttgg cggcatgact caaccaaa
//
LOCUS       DAOREF010000775         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_66472_length_1646_cov_3.884349, whole genome shotgun sequence.
ACCESSION   DAOREF010000775 DAOREF010000000
VERSION     DAOREF010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66472_length_1646_cov_3.884349"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..863)
                     /locus_tag="PKI33_14135"
     CDS             complement(168..863)
                     /locus_tag="PKI33_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM38204.1"
                     /translation="MSLQQVVPGFHCINLGFVNAYLLEMQDGLVLIDTGIPNSAEKIL
                     SSVASLGKQPSDIKHILVTHLHGDHTGSLAAIKKATGAPVSMHTLDAQLVRIGQAGRP
                     VQPAPGLFNRFIVFMMSRRGPIPIEPAETENELSDGQILEAVAGIKVIHAPGHAAGQV
                     AFLIPIHGGILVAADSCNNRSGLGFPPIFENMDDGIQTLKKLSALDFEVAVFGHGKPI
                     TSGASKAFKEKWG"
     gene            959..>1646
                     /locus_tag="PKI33_14140"
     CDS             959..>1646
                     /locus_tag="PKI33_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNM38205.1"
                     /translation="MNKNLENDQNLISSLVEHTKDIAQNYLNEIDQRPPATAYVQKQN
                     LGLSLTGIGAEKTLDLFMERYGNHMPASNGPRFWGLVTGGTTPASLLGDWLTSTYDLN
                     LSHAGNSPAPDIEMEAISMLRELFGLPASFSGTFVTGATMSNFAGLAIAREWIGRQHG
                     KSVSQDGLHAIPPVKILSAEAHASSFKSLGMLGLGRNSLKVIPKLPENREAVNVVELR
                     KALEEMNGEPC"
BASE COUNT          454 a          415 c          382 g          395 t
ORIGIN      
        1 agatcggctc gcgcatgaaa gccaccatct ccgttgacca ccgcgtcagc gacggcgccg
       61 aagccgccca gttcatgcaa gccctggctg ggttccttga aaatccggtc agaatgttgg
      121 tataaaacaa aaactccgag caaattgctc ggagtttttt attgggctta gccccatttt
      181 tctttgaatg cttttgatgc gccgcttgtg atcggttttc catgtccaaa tacggcaact
      241 tcaaaatcca aagcggataa ttttttgagt gtttgaattc catcgtccat gttttcaaaa
      301 ataggcggga atcccaaacc agagcgatta ttacaagaat cggcggcaac caaaatccca
      361 ccgtgaatgg gaatcaagaa agcaacctgc cctgcggcat gacccggcgc atggatcact
      421 ttaatccccg caaccgcttc aagaatttgc ccatcgctta attcattttc ggtttctgca
      481 ggttcaatgg gaatgggtcc acggcgagac atcataaaaa cgataaagcg attgaacaac
      541 ccgggtgcag gttgaacagg gcgcccagcc tgaccaattc gtaccaactg agcatccagt
      601 gtgtgcatag aaactggggc acccgtcgcc tttttaattg cagccaggct gccggtatgg
      661 tctccatgca aatgagtgac aaggatatgt ttaatgtcgc tgggctgttt ccctaaagat
      721 gctacggaag atagtatttt ctcagcgcta ttaggaatgc cagtatcaat caagaccaat
      781 ccgtcttgca tctccaagag atacgcattt acaaaaccaa ggtttataca atgaaaaccg
      841 ggaacaactt gttgaagaga cataattcct cacagaatca agatttttcg atcacagtat
      901 tgaggaatat ccaatcgagt aaaatcttac gtgaaaatca atcctgaaag aaatccttat
      961 gaacaaaaac cttgaaaatg accaaaacct tatatcttct ttagtagaac atacaaaaga
     1021 tatcgcacaa aactatctta acgaaattga ccaacgcccg cctgcaacag cgtatgttca
     1081 aaaacaaaac cttggtctct cccttacggg aatcggcgca gaaaaaacat tggacctttt
     1141 catggagcgc tacgggaatc acatgcccgc cagcaatgga ccgcgattct ggggtttggt
     1201 cactggcggc acgacgcccg cctccctgct cggggattgg ctgacttcta cctatgacct
     1261 gaatttgagc cacgctggga attctcccgc accggatatc gaaatggaag cgatttcgat
     1321 gctgcgtgag ctcttcggtt tgcccgcttc tttctcaggg acatttgtta ccggtgcgac
     1381 gatgtcgaac ttcgccggtc tcgccatcgc ccgcgaatgg atcgggcgcc agcatggcaa
     1441 gtccgtttcg caggatggct tgcacgctat tccacccgtg aaaatcttga gcgctgaagc
     1501 ccatgccagt tcattcaagt cgttgggcat gcttgggctg ggaagaaact ccctgaaagt
     1561 tatccccaaa ctgcccgaaa acagagaggc ggtgaacgtc gtcgaattgc gaaaggcttt
     1621 ggaagaaatg aacggcgaac cgtgta
//
LOCUS       DAOREF010000776         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_66540_length_1645_cov_4.024528, whole genome shotgun sequence.
ACCESSION   DAOREF010000776 DAOREF010000000
VERSION     DAOREF010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66540_length_1645_cov_4.024528"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKI33_14145"
     CDS             complement(<1..285)
                     /locus_tag="PKI33_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38206.1"
                     /translation="MKSSNLFVWALCLAVFISACGAAAPTQAPQPFVLITAAPNASPT
                     PTPFQPSLASPTAPFNTSGSNPAPQPILATETTFPTATPQASPTATAPIEQ"
     gene            complement(312..1121)
                     /locus_tag="PKI33_14150"
     CDS             complement(312..1121)
                     /locus_tag="PKI33_14150"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNM38207.1"
                     /translation="MRGVRDFGNALVAAILSIGLTLGALSISLVGFIPEEVPTPSPTL
                     IVSPIPVTATNTLPPTATLLVTVNIPTSSPTNTIVPPTSCQPPVGWVGVVVQVGDTLD
                     SLAARYGTYKDILKNKNCLFTDSLIAGTLLYVPGGISTSTVVACNQGAVGWIKNYMVR
                     PGDTFYNIGTRYGVNANLIKLVNCRTSDIIYAGEILYVPNVPTRTPTFTPLPGITYTA
                     APLLTEPLTLTVLPFTATYPPTQTSTATTAPTVSPIPTQTASLTAIPTPTP"
     gene            complement(1125..>1645)
                     /locus_tag="PKI33_14155"
     CDS             complement(1125..>1645)
                     /locus_tag="PKI33_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNM38208.1"
                     /translation="LYSLNPEAVDLNSIIDNAMSYTSSQIREKNISIHLEVPKAVTSI
                     HADREALQQIVIHLLQNAGAATPFEGTIRLKVQTQNEDGAEYILIQVADQGGGIASED
                     IPRVFTRLYRADNVLIQGVGDTGVGLSIAKTLTEAQHGRIWVESKVGEGSTFSVLLPI
                     SGDKLADKQKRK"
BASE COUNT          409 a          321 c          494 g          421 t
ORIGIN      
        1 ctgttcaatg ggggctgtcg ctgtcggtga ggcttgaggc gtggcagttg ggaatgtagt
       61 ttcagttgca agaatcggtt gcggggcggg gtttgatccg cttgtattaa aaggtgcagt
      121 tgggctggct aaggaaggtt gaaagggtgt tggtgtaggc gatgcgtttg gagcggcagt
      181 gattaaaaca aaaggctgag gcgcttgggt cggagccgct gcaccacaag ctgaaataaa
      241 aacggcaagg caaagcgccc atacaaaaag gttggatgat ttcatcgatg tgttatggaa
      301 agtgcgcctg cttagggcgt tggggtgggg atagcggtta agctggcggt ttgggttgga
      361 atgggcgaaa ccgtaggtgc ggtggtggcg gtggatgttt gggttggcgg gtatgttgcc
      421 gtgaaaggca gaaccgttag ggttaatggt tcggtgagta atggtgccgc ggtgtaggta
      481 ataccaggca aaggtgtaaa ggttggtgtg cgtgtgggta cgtttggtac ataaagaatt
      541 tcacccgcat aaataatgtc gctagttcgg cagttgacaa gtttgattaa gttggcgttg
      601 acgccatagc gagtgccgat gttatagaag gtatcgcctg ggcgaaccat atagtttttg
      661 atccaaccaa cagcgccttg gttacaagca actactgtac tggtggaaat gccacccggc
      721 acatatagaa gcgtgccggc aataagactg tctgtgaaaa ggcagttttt gtttttgaga
      781 atgtctttat acgtgccata ccttgctgcg aggctatcta acgtgtcgcc tacttgaaca
      841 acaactccca cccagcccac aggtggttga caggaggttg gaggaacaat ggtattggtt
      901 ggtgaagacg taggaatatt aacggtgact aacaaagtag ccgttggagg caaggtgttt
      961 gtagcagtta ccggaatagg agaaacaatc agtgttgggg atggagtagg gacttcttca
     1021 gggataaatc caacaagaga aatcgacaac gctcccagtg ttaacccaat tgagagaatt
     1081 gccgcaacaa gcgcgtttcc aaaatcgcgc acgcctcgca ttgtttattt ccttttttgc
     1141 ttgtcggcaa gtttatcgcc agagattggc aaaagcacac taaacgtgga gccttctccc
     1201 actttgcttt caacccaaat acgaccatgc tgggcttcgg tgagggtttt ggcaatggaa
     1261 agaccaacac cggtatcacc aaccccttga atgagaacat tatcagctcg atagagacgg
     1321 gtaaaaacgc gcggaatatc ttcggaggca atcccgccgc cttgatctgc gacttgaatc
     1381 aaaatgtact cagcgccgtc ttcgttctgg gtctggactt tgaggcggat cgtcccttcg
     1441 aagggagttg ccgctccggc gttttgcaac aagtgaatga caatttgttg tagggcttcc
     1501 ctgtccgcgt ggatggatgt gactgccttt ggaacttcca gatgaatgga aatatttttc
     1561 tcacgaatct gagagctcgt gtacgacatg gcattgtcga tgattgagtt caaatctaca
     1621 gcttcaggat tgagcgaata caggt
//
LOCUS       DAOREF010000777         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67011_length_1639_cov_5.303662, whole genome shotgun sequence.
ACCESSION   DAOREF010000777 DAOREF010000000
VERSION     DAOREF010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67011_length_1639_cov_5.303662"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..694)
                     /locus_tag="PKI33_14160"
     CDS             complement(155..694)
                     /locus_tag="PKI33_14160"
                     /inference="COORDINATES: protein motif:HMM:NF017987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNM38209.1"
                     /translation="MFQTNISLSPEMTANERNQAIREAYNQDRSQMTLMLAHQLRIPE
                     VDILRALEGDTARELDFARWEEIIRAFEALGEVTVIVSNGSTTIESFGQFGGFSNAQG
                     FLNVRSKSLDMHIRGWELASVFAFRKPSHLDKHESLSFQFFDKRGNSAFKVFLNFGGK
                     DPAPELVEAYNELIGRFSK"
     gene            858..1559
                     /locus_tag="PKI33_14165"
     CDS             858..1559
                     /locus_tag="PKI33_14165"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNM38210.1"
                     /translation="MSVSLAMQRYAAEIYRLQQDHEQVPLSLLSSHVDASAQAISNMV
                     KRLQKLGYLVHEPYRGVRLTETGEKIAMPSLRRHRLTEVFLVKVMKYDWATAHELSDT
                     FEKGVNDEIENRMDELAGRPTRCPHGEPIPSKDGFMPVVKDMPLIDVPSGSDCVISRV
                     RTHDLEKLNYIGELGLTPGKQFHLFSCAPFKGPLRLQMKPHDHLIGYELAASLWVEVT
                     LKGEGNKVPTSLASK"
BASE COUNT          422 a          380 c          404 g          433 t
ORIGIN      
        1 tcttttgatg aacatagcat ttcctgaggt tgaggattaa acaaaaggaa gggcggtttg
       61 ggcgccctca cctcttggtt gatattgtag gctcaagttt actaaggagt atatagattt
      121 atatagaatt tcactagagt tttctgagga agatttactt tgaaaaacgc ccgatcaatt
      181 cgttataggc ttcgaccaac tcaggagcag ggtctttgcc gccaaagttg agaaagactt
      241 tgaaggcaga gttgccgcgt ttatcgaaga attggaagga aagcgattcg tgcttgtcga
      301 ggtggctcgg tttgcggaaa gcgaagaccg atgccagctc ccaaccgcga atgtgcatat
      361 ccagtgattt gctgcggacg ttgagaaagc cttgagcatt ggaaaaaccg ccgaactgac
      421 cgaacgattc gatggtggtc gagccgttcg agacgatgac ggtcacctcg cccaacgcct
      481 caaatgcgcg gatgatctcc tcccagcgcg caaaatcaag ctcacgggcg gtgtcgcctt
      541 cgagcgctcg cagaatgtcc acttcgggga tgcgtaactg gtgggcaagc atgagtgtca
      601 tttgagagcg gtcttggtta tacgcttcac gaatcgcttg gtttcgctcg ttggcggtca
      661 tttcggggct gaggctgata ttggtttgga acatgaaata ttctcctaaa atgtagtatt
      721 gaccatactt tgtttgataa gtggcgcgta gtataatcac ggcgattcga ataatctaga
      781 taattgttat aaaatttatc tatatccagt tgaagccagt tttcaaccca attgaagatt
      841 ttggagtgtt ttaaagaatg tctgtaagtt tggctatgca gcgttatgcc gcggagatct
      901 accgcctgca acaagaccat gagcaggtgc cgctttcgct tttaagttcg catgtggatg
      961 cttctgcaca agccatttcc aacatggtca agcgtttgca aaagctgggt tatcttgtac
     1021 atgaacccta tcgcggagtc cgccttactg aaactggcga aaagatcgcc atgccttccc
     1081 tgcgtaggca tcgcctcact gaggttttcc tcgtcaaagt gatgaagtac gactgggcaa
     1141 ctgcccacga actttctgac accttcgaaa aaggcgtcaa cgacgagatc gaaaaccgca
     1201 tggatgaact tgccgggcgc cccacacgtt gcccgcatgg cgagccgatt ccttccaaag
     1261 atggcttcat gcctgttgtc aaagatatgc cattgatcga cgtcccctct ggttctgatt
     1321 gcgtcattag ccgcgtccgc acccatgacc ttgaaaaatt aaattacatt ggcgaattag
     1381 gtcttacccc cggcaagcaa tttcacttgt ttagttgcgc accgttcaaa ggtccgttac
     1441 gattgcaaat gaaaccgcat gaccatctca tcggttacga actcgccgct tccctctggg
     1501 tggaagtgac cttgaagggc gaaggaaata aagtgccaac aagcctggcg tctaaataaa
     1561 aaatacatcc cgatttcggg atgtattttt ttatcaagaa aatcactagc tcaatatcac
     1621 acgcacacga acttctttg
//
LOCUS       DAOREF010000778         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67107_length_1638_cov_2.800379, whole genome shotgun sequence.
ACCESSION   DAOREF010000778 DAOREF010000000
VERSION     DAOREF010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67107_length_1638_cov_2.800379"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKI33_14170"
     CDS             complement(<1..540)
                     /locus_tag="PKI33_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM38211.1"
                     /translation="MLNIVETENKHTSGVYAKQTLTIVRGQGASLFDVDGNEYLDCSS
                     GHGVANLGHAHPKVAEALYKQANTLITLFETFPNDQRAALMEKITALVPGMDRVFFCN
                     SGTEAVEASFKFSRISTGRKNIIAAMRAFHGRTYGSLSATFNKKYREGFEPLLPGVSH
                     VSYNNIEALEKAVNEETAAV"
     gene            complement(554..>1638)
                     /locus_tag="PKI33_14175"
     CDS             complement(554..>1638)
                     /locus_tag="PKI33_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNM38212.1"
                     /translation="PSFVLGGRAMALVESEDNLAGFIQDAMEAAPGQPILIDKFMDDS
                     FEVDVDALADGERVVVGAIMEHIEEAGVHSGDAACVLPPYKVSAYHLEIMREYTEQLG
                     LALGVRGLMNVQFALKDEIVCVLEVNPRASRTVPYASKATNLNLAYKAAQVMAGKTLP
                     ELNVLQEPRVDGFFVKEAVMPFKKLPGTSIILGPEMRSTGEVMGHASRFGHAFAKSQT
                     AAGTGLPQSGAVLITVNDFDKSAGLKFARDMHNLGFKLYATPGTADICAKAGLPVEVV
                     EKAQDGSTQIVDLIRTGKIDLVLNTPLGANAHTDGVEIRRAAIAMNVPLLTTLTAAMA
                     AVSAIRALSKKELKYRSLQSHFAEKI"
BASE COUNT          424 a          442 c          361 g          411 t
ORIGIN      
        1 cacagccgca gtttcttcat tgacggcttt ctccaaagct tcgatattgt tgtatgaaac
       61 atgcgaaaca ccgggaagaa gtggttcgaa gccttcacga tattttttgt tgaaggtggc
      121 agagagcgag ccgtaggttc gtccatgaaa agcacgcatg gcggcgatga tgtttttacg
      181 cccagtggaa atgcgcgaga acttgaacga tgcttccact gcctcggtgc ctgagttgca
      241 aaagaagact ctgtccatgc cggggacaag cgcagtgatc ttttccatta acgcggcgcg
      301 ttggtcattc ggaaatgttt cgaacaacgt aatgagcgtg ttcgcctgtt tgtaaagcgc
      361 ttcagcgact tttggatgtg catgacccaa gttcgccacg ccgtgaccag aggaacaatc
      421 gagatattca tttccatcta catcaaaaag cgaagcaccc tgtccgcgaa cgatggtcag
      481 ggtttgtttg gcatatacac ctgatgtgtg tttgttttca gtttcaacga tatttaacat
      541 gattacctct caattaaatt ttttccgcaa aatggctttg cagactacga tatttcaatt
      601 cttttttact aagtgcgcga atggcagata ccgcagccat ggctgcggtc aacgtagtaa
      661 gtaagggcac attcatcgcg atagctgcgc gacgaatctc cacgccatcg gtgtgcgcgt
      721 tcgcaccaag tggagtgttc aacacaaggt caatcttccc tgtgcggatc aaatcgacaa
      781 tctgcgttga cccatcttgc gctttctcaa ccacttcaac tggtaagcca gcctttgcac
      841 aaatatccgc agtaccgggc gtggcataca atttgaaacc taaattatgc atatctcgtg
      901 caaacttcaa accagcactc ttatcaaagt cattcaccgt gatcaagacc gccccactct
      961 gtggaagtcc cgtcccggca gcagtttgtg atttagcaaa ggcatgacca aaacgcgagg
     1021 catgccccat cacttcgccg gttgagcgca tttccggtcc gagtatgata ctggtaccgg
     1081 gcaacttctt aaacggcatc acagcttctt tcacaaagaa accatccact cgcggttctt
     1141 gcagaacatt caactcaggc aaggtcttgc ccgccatcac ttgcgcggct ttataagcaa
     1201 gattcaaatt cgtggctttg ctagcatatg gaaccgtccg tgaagcgcgt gggttgactt
     1261 ctaacacgca aacaatctca tctttgagtg caaattgtac gttcatcagt ccgcgcacgc
     1321 caagggcaag cccaagttgc tcggtatatt cgcgcatgat ctcaagatga tacgcactga
     1381 ctttatacgg cggcaacaca caagccgcat caccagaatg aacacccgcc tcttcaatat
     1441 gttccatgat agcgcccacc actacacgtt cgccatctgc caatgcatcg acatctactt
     1501 caaacgagtc gtccataaat ttatcgatca aaatcggttg acccggcgcc gcctccatcg
     1561 catcttgaat gaatcctgcc aggttatctt cagactccac caaagccata gctcgcccac
     1621 ccaacacaaa cgatggac
//
LOCUS       DAOREF010000779         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67250_length_1636_cov_3.629349, whole genome shotgun sequence.
ACCESSION   DAOREF010000779 DAOREF010000000
VERSION     DAOREF010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67250_length_1636_cov_3.629349"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..528)
                     /locus_tag="PKI33_14180"
     CDS             complement(163..528)
                     /locus_tag="PKI33_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM38213.1"
                     /translation="MPIPLNTILIYAKNMKATAEFYQKYFGFVSTGEVVEGLIELTSA
                     HGGANILIHQAAKSIKLGQVGVKLMFDIEDIETFKKEAAAQGLKFGSTHQANGYSFAN
                     AKDPDKNPITISSRAYRRK"
     gene            complement(551..1213)
                     /locus_tag="PKI33_14185"
     CDS             complement(551..1213)
                     /locus_tag="PKI33_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNM38214.1"
                     /translation="MTTLTQTTENKYWHVALWLAFFTIFYNFAEGLISTYFGAQDETL
                     TLFGFGVDSFIEVASGVGILAMIFRIRQNPNTSRSQFEQTALRITGTSFYLLAAGLGI
                     TGIYNLFTVHKPATTLPGLIISLVSITVMWALVLGKRKVGRALHSAPILADANCTMVC
                     IYMSVILLASSLIYQFTGFGRIDSLGAFGLIYFSINEGKEAFEKAANVNALCCDDDDC
                     KE"
     gene            complement(1251..1616)
                     /locus_tag="PKI33_14190"
     CDS             complement(1251..1616)
                     /locus_tag="PKI33_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM38215.1"
                     /translation="MATFRYLVHDVESAIHFYTTHLGFKLIDQMGPAFAIVAKEDLNL
                     WLSGPQTSAARLMPDGRKPEAGGWNRFVIEVENIESLVEEMKQTGVSFRNEIISGPGG
                     KQILVEDPSGNPIEIFQPA"
BASE COUNT          426 a          363 c          402 g          445 t
ORIGIN      
        1 atgaaaatat cgaggttggg ggtgcggtcc accggttcac cggcgatgac attttttatg
       61 cgttgcagag agttcatagt agcaccttgg gagaggattt ttttctatga tacccccaat
      121 gcgcagactt ggcgtatgcg cccagtattt ttatctgaaa ccctactttc gccgataagc
      181 acggctggaa atggtaatcg ggtttttgtc tgggtctttg gcgtttgcaa aagaataacc
      241 atttgcttga tgggtactgc caaacttaag accttgtgct gccgcttctt tcttgaatgt
      301 ttcaatgtct tcaatatcga acatgagctt tacgccgact tgacctaact ttatgctttt
      361 ggctgcctga tgaatcagaa tatttgcccc gccatgcgcg gacgttaatt caataagccc
      421 ttccaccact tcgccggttg aaacaaagcc aaagtatttt tgataaaact ctgcagtggc
      481 tttcatattc tttgcataaa tcaggatggt attgagggga attggcattc gattttctgt
      541 tcctgcgttt ctattctttg caatcatcat cgtcacaaca aagtgcgttg acattggctg
      601 ctttttcaaa ggcttctttg ccttcgttga tggagaagta aattagacca aaggcgccga
      661 gactgtcgat tcgcccaaag cctgtgaatt gatagataag gctggatgcc aacagaataa
      721 cggacatgta aatacaaacc atggtgcagt tggcatctgc caaaatggga gcggaatgaa
      781 gcgcacgccc tactttacgc ttccccagca ccaatgccca catgaccgtg atggaaacga
      841 gtgaaatgat cagccccggc agagttgtag ctggcttgtg gaccgtaaaa agattgtaaa
      901 tgcctgtgat gccaagaccg gcagccagca gataaaagga tgtgcctgta atgcgcaagg
      961 cggtctgttc gaattgagaa cgggaggtat tggggttttg gcggatgcga aatatcatgg
     1021 caaggatgcc gactcctgat gcgacctcaa tgaatgagtc gacgccgaaa ccaaagagtg
     1081 tcagggtctc atcttgtgcg ccaaaatagg tggagataag cccttcggca aagttataaa
     1141 agatcgtgaa gaatgccagc cagagcgcaa cgtgccaata tttattttcg gtcgtttgag
     1201 taagggtcgt catgtggata ctcctaaggt tttatacaat accatgctta ttatgcgggt
     1261 tgaaagatct ctatcgggtt gccagacggg tcttcgacaa ggatctgttt cccaccagga
     1321 ccagaaataa tctcatttcg aaacgatacg ccagtctgct tcatttcttc taccagagac
     1381 tcaatgtttt ctacttcgat cacaaaacgg ttccagccac cggcttctgg ctttctgcca
     1441 tcgggcatga ggcgcgctgc agaggtctgc ggaccactca gccataggtt taaatcttct
     1501 ttcgctacga tcgcgaaggc aggacccatt tgatcaatga gtttgaagcc aaggtgggtg
     1561 gtgtagaaat ggatcgcgga ttcaacatca tgtaccagat aacgaaaagt tgccataggg
     1621 tctccagact tctaaa
//
LOCUS       DAOREF010000780         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67319_length_1635_cov_4.637975, whole genome shotgun sequence.
ACCESSION   DAOREF010000780 DAOREF010000000
VERSION     DAOREF010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67319_length_1635_cov_4.637975"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1334)
                     /locus_tag="PKI33_14195"
     CDS             complement(24..1334)
                     /locus_tag="PKI33_14195"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM38216.1"
                     /translation="MESQFFVAVIGAGPAGLFGARELANSGVRVALFNRDIKAGGLAE
                     YGIYLEKHTMKEGLRKQFRQALDMPNIDYYGNVAIGTKGDFTLDEIRALGFDAVLVAA
                     GAQGTKSLGLPGEELEGVYHAKDVVYGYNKLPPFSQRKFRYGKRAAIIGAGNVMVDIA
                     RYLINHQGVEEVTAVVRRGPAEVNFTKEEMKHLVAYLDMDEFEKELARVRPALDAIGQ
                     DPQIGRQKVLDALAKADPKVKEAKFKFDFLSSPSGMFGENGLLTQLEVEENILTERDG
                     KTSAKGTGKKHTLDVETVIFAIGDQVDANFGLPTEWGEFSKTKEPKFPIEGTSYESTT
                     EGIFVGGWSRKASEGLVGYARRDGTNAAKAVMQYLQTKQPSNASPEAVAAKVKALQKP
                     IVYKEDIKRLEAAEAEEAKKRELEWFKYGSNEEMLQAMGLIETA"
BASE COUNT          391 a          447 c          366 g          431 t
ORIGIN      
        1 tgaagaactc ggagattgat ttcttacgcg gtttcgatca gacccatggc ttgcaacatt
       61 tcttcgttgc tgccgtactt gaaccattca agttcacgct tcttggcttc ttcggcttca
      121 gccgcttcga gccgtttgat atcttctttg tacacgatcg gcttttgtag cgctttgacc
      181 ttggctgcca ccgcttccgg gctggcattg ctgggctgtt tggtctgtag atattgcatc
      241 acggctttgg cggcattggt gccatcgcgg cgagcatacc caaccaaacc ttcgcttgcc
      301 ttacgagacc atccgcccac gaagatgcct tcggtcgtgg attcgtagga agtgccttcg
      361 atggggaact tcggctcttt agttttggag aactcgcccc attcggtcgg cagaccaaaa
      421 tttgcatcta cttggtcgcc aatggcgaag atgactgttt caacatcgag cgtatgcttt
      481 ttgccggtgc ccttggcgct ggtttttcca tcgcgttcgg tcagaatgtt ctcttccact
      541 tcgagttggg tgagtaaacc attctcgcca aacatgccag aaggggagga gaggaagtcg
      601 aacttgaact tcgcttcttt caccttcggg tctgctttgg caagcgcatc caaaaccttt
      661 tgacgcccaa tctgcgggtc ctgaccgatc gcatcgagag ccgggcgtac acgagccaat
      721 tccttctcga actcgtccat gtcgagataa gcgacgaggt gcttcatttc ttccttcgtg
      781 aaattcacct cagccggacc gcgacgaacc acggcggtca cttcttctac accttggtgg
      841 ttgatgagat aacgagcaat gtccaccatc acgttaccag cgccgataat ggcggcacgc
      901 tttccatagc ggaacttgcg ctggctgaac ggtggcaact tgttatagcc ataaaccacg
      961 tctttggcgt ggtacacgcc ttcaagctct tcgccgggca agcccaaaga cttggtgccc
     1021 tgcgcacccg cagcaaccaa caccgcgtca aaaccgagcg cgcgaatttc atccaatgtg
     1081 aaatcaccct ttgtgccgat ggctacattg ccataatagt caatgttcgg catgtcgagc
     1141 gcctgacgga actgcttgcg cagcccttct ttcattgtgt gtttttcaag gtaaatgccg
     1201 tactcagcca gaccaccagc tttgatatca cgattgaaaa gagcaacgcg cactccgcta
     1261 tttgctaatt cacgtgcccc gaaaagtcct gcgggtcccg cgccaatcac ggcgacaaaa
     1321 aattgactct ccacgttctt cctccgaaat aaaaaaaatt acattttaga caaaaactat
     1381 caggttttac cacaaacatc acagaattta cagacaaaaa accgttcaat tctgttatca
     1441 cagtcgaatg ctacctgcaa aaagttactt ttcagatgtt aattccgcat tagagatgtt
     1501 tgtgcttgct gtaagaaacc caccccatct tcataaacat ctatgctaat attaatccat
     1561 gtgctaacca agaggcagta tgacagacga acccatcaaa gaagatcctg tacaaaaatt
     1621 ccgaaagctt attgc
//
LOCUS       DAOREF010000781         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67342_length_1635_cov_3.041139, whole genome shotgun sequence.
ACCESSION   DAOREF010000781 DAOREF010000000
VERSION     DAOREF010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67342_length_1635_cov_3.041139"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /gene="mreB"
                     /locus_tag="PKI33_14200"
     CDS             <1..759
                     /gene="mreB"
                     /locus_tag="PKI33_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNM38217.1"
                     /translation="VPLPRPRVIVGLPTGVTEVEKRAVYDAVMASGARQAMLIEEPIA
                     AAIGAGLPIGEVRGSMVVDIGGGTTEVAVMSTGGVVASRSLRVAGDEMDQDVVQYLRN
                     KYNLLVGTGIAEQIKWQIGSAYPLQPEKTMEVRGRNLVTGLPETVEISSVEIREALSG
                     SVQVIIDTVRDALDEIPPEIVSDLMDIGICLAGGGALLQGLAERLTDELKLRVWVAED
                     PLTCVARGAATVFEDLESLSRYLIGLERGSTRHV"
     gene            796..>1635
                     /gene="mreC"
                     /locus_tag="PKI33_14205"
     CDS             796..>1635
                     /gene="mreC"
                     /locus_tag="PKI33_14205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNM38218.1"
                     /translation="MNSRSLQTTIIFLVIGGILVLAFSGALGSASRGFTSVLVDVQTW
                     ISSRFLGFQDFVTAPRDIVTLRTRNAELEAQVSQLQAQLIELQQRVNETEILAALVDF
                     SRSNPESTYKAASVIGRDPSPFLHYIIINRGSNDDIRRGMPVVTNQGLVGRVDAVIAD
                     AARVQLITDPSSSVNVYLQNADTSAVLYGSVTGDVSLDLISQNVSVQAGDLILTSGLG
                     GGYPADLLIGQVVTVRSLEFELFQQATVQPAVDFSRLEIVLVITNFRPVDIAPLEPVV
                     TPSP"
BASE COUNT          361 a          369 c          432 g          473 t
ORIGIN      
        1 gtgcctttgc cacgtccgcg tgtgatcgtt ggtttaccta ccggtgtgac cgaagtggaa
       61 aagcgcgctg tgtatgatgc agtaatggca tcaggcgcgc gccaagctat gttgattgaa
      121 gaaccaattg cggcggcgat cggagccggt cttccaattg gagaagtccg cggttctatg
      181 gtcgttgaca ttggtggcgg cacaactgaa gtcgcagtca tgtctacggg tggtgtagtg
      241 gcgtcacgtt cattgcgcgt ggctggtgat gaaatggatc aagatgttgt gcagtatttg
      301 cgcaacaaat acaatttatt agtggggacg gggattgctg aacagatcaa atggcaaatt
      361 gggtcggcat atccattgca acctgaaaaa accatggaag tgcgcggcag aaatttagtg
      421 actggtttac ctgaaacggt tgaaatttct tctgttgaaa ttcgcgaagc gctctctggt
      481 tcggtgcaag tgatcattga taccgttcgc gatgccttgg atgaaattcc gcccgaaatc
      541 gtttcagact taatggatat tggtatttgt ttagccggcg gcggcgcgct cttgcaaggc
      601 ttggcagaac gtttaaccga tgaactgaaa ttacgtgtat gggttgccga agacccactt
      661 acttgtgtag cacgtggtgc agccactgta ttcgaagacc ttgagagcct ttctcgctat
      721 ttgatcggtc tcgaacgtgg cagtacgcgt cacgtatagg attaagtaga cagcctttcg
      781 tgggctgaat aaaaaatgaa ctctcgttcc ttacaaacga caattatttt tctggtgatt
      841 ggggggattc tagtgcttgc ctttagcggc gcattaggtt cagcatctag gggatttact
      901 tctgtgcttg ttgatgtcca aacatggata tccagccgtt ttcttggctt tcaagatttt
      961 gtcaccgcgc ctcgtgatat tgtgacgttg cgtacgcgta atgcagagtt ggaagcacaa
     1021 gtttctcagt tgcaggctca acttatcgag ctacaacaac gtgtcaatga gaccgaaatt
     1081 cttgcggctc ttgttgattt ttcccgctcg aatcccgaaa gtacatataa agccgcatcg
     1141 gttatcggtc gtgacccaag cccgtttttg cattacatca tcatcaaccg tggctcgaat
     1201 gacgatattc ggcgtggtat gcctgttgtg accaatcagg gtctcgtcgg gcgtgttgat
     1261 gcggtcattg cagatgctgc tcgtgtgcaa ttgattacgg atccgtcgtc ttctgtgaat
     1321 gtctatttgc aaaatgcaga tacaagtgcc gtcttatatg gttcggtcac tggcgatgtg
     1381 tcccttgacc ttatctctca aaatgtctct gtgcaagcag gcgatttgat ccttacatca
     1441 ggtttgggcg gaggataccc cgcagacctc ctaattggac aagttgtcac ggttcgttct
     1501 ttggaatttg agctgtttca acaagcgaca gttcaacccg ccgttgactt ttcccgcttg
     1561 gaaatcgtcc ttgtcatcac caacttccgc ccagtggaca ttgctccgct tgagcccgtg
     1621 gttacacctt ccccg
//
LOCUS       DAOREF010000782         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67400_length_1634_cov_6.032932, whole genome shotgun sequence.
ACCESSION   DAOREF010000782 DAOREF010000000
VERSION     DAOREF010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67400_length_1634_cov_6.032932"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1492)
                     /locus_tag="PKI33_14210"
     CDS             complement(62..1492)
                     /locus_tag="PKI33_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38219.1"
                     /translation="MTKTSKRFSAFSISWLLLITGIMSIMVGCASPTQTPTLPVPTKL
                     IIQPTIESTVSPTQIPLSPTGSWLAFTADNYLFAVNKDGTGLTSLAPELSVMMFNRQP
                     SSNTITAITSSEESQSLAITFIELPSKNIIHEIPLLSFYADGKIKLDIGSTGALGGIQ
                     GGIQWSPNGEYLAFMGAIENSQSSLYVYDSKLQVVKKLSENQYQAANPTWSPDGKWII
                     FEDVSDFLGWQTESVWATSVDDGKTKLLYKADQTEQRILGWVGHDNFVVTDHSMDGNR
                     NIRMVNVVTGKINDLYSGYFTLAAVSKNGTVIFLPTSGAPGAGSTLESGFYLISPNTT
                     SLKLIHKLHETENVVSLDIIWDSSNELFITGFRCDNEPEKAVAFDITGKEVCVTNLKS
                     NFSPNGDWYIIIENGQLNIYKSDGNKLGEINENGRSIVWDPSSDGFFTVTNNKELNYV
                     SLSSMNSQSVYKSRYGFFGFTWIGVP"
BASE COUNT          455 a          334 c          334 g          511 t
ORIGIN      
        1 aactgcttgg gatgtgcgca gaatccccag cgtcaggtgc acgctttgtt gggtggcggt
       61 tttagggaac tccaatccat gtaaaaccga aaaagccata tctgctctta tatacacttt
      121 gcgagttcat acttgaaaga gaaacgtaat taagttcttt attatttgtt actgtgaaga
      181 aaccatctga gcttggatcc caaacaatac ttcttccatt ttcattgatt tctcctaatt
      241 tatttccgtc cgacttatag atattaagtt gcccattctc tataataata taccaatcgc
      301 catttggcga aaagttagat ttcaaattcg taacacatac ctccttacct gttatatcga
      361 acgccactgc tttttcaggt tcgttatcac acctaaatcc agttataaaa agttcatttg
      421 atgaatccca aattatatct agtgagacta cgttttcagt ttcatgtaat ttgtgtataa
      481 gtttcagact tgtagtgttg ggcgatatta gataaaaacc tgattctagt gtagaccccg
      541 ccccaggggc gccagaggta gggaggaaaa tcactgtgcc attcttgcta acagccgcta
      601 gtgtgaaata acctgaatat aggtcgttta tttttcctgt tacaacatta accattcgaa
      661 tatttctatt tccatccatg ctatggtctg tgacaacaaa gttgtcgtgt ccaacccacc
      721 ccaagattct ttgttcagtt tgatctgctt tatacaggag ttttgtttta ccatcatcaa
      781 ctgacgttgc ccaaacgctt tctgtttgcc acccaagaaa atcggacaca tcttcaaaaa
      841 taatccattt tccgtcgggt gaccaagttg ggttagcggc ttgatattga ttctctgaaa
      901 gtttttttac aacctgtaat ttactgtcat atacgtacaa ggatgattgc gaattttcta
      961 tagcacccat aaaagccaaa tactccccgt ttggagacca ttgtatacct ccctgtattc
     1021 ccccaagagc tccagtagaa ccaatatcta actttatttt tccatctgca taaaaagata
     1081 gcaaggggat ttcgtgaata atatttttac tagggagttc tataaaagta attgccaaag
     1141 attggctttc ttcacttgat gttatagcgg tgatggtgtt tgatgatggc tgtctattaa
     1201 acatcatcac agataattca ggggctagag atgtcaagcc tgttccatct ttgttgacag
     1261 cgaacaaata attgtcggcg gtaaaagcca gccaagaacc tgttggcgaa agtgggattt
     1321 gcgttggtga tacagtgctt tcaattgttg gttgaataat aagttttgta ggcactggca
     1381 aagttggtgt ttgagtcgga cttgcgcagc caaccataat agacataatg cccgtgatta
     1441 gcaaaagcca agatattgaa aatgcagaaa atcgtttcga tgtttttgtc atggtgaatt
     1501 cagccaccca acggtttgcg ttacctgcgc tggggcgggg acggcgaagc cgtccaacca
     1561 gaaaaatgat aaggcgtgtg aaactgcttg ggatgtgcgc agaatcccca gcgtcaggtg
     1621 cacgctttgt tggg
//
LOCUS       DAOREF010000783         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67648_length_1631_cov_3.701142, whole genome shotgun sequence.
ACCESSION   DAOREF010000783 DAOREF010000000
VERSION     DAOREF010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67648_length_1631_cov_3.701142"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKI33_14215"
     CDS             complement(<1..675)
                     /locus_tag="PKI33_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38220.1"
                     /translation="MTIYSGLRIAFGKDQPHVQFTVEMDPPSVYWVYRIKSSEIENLI
                     QKLRIPPNLSLTPVRCLDIDEPSYYLALNAYRVSGLVSGVRAEWSVFVRDSTNTARYM
                     IVDARSSTTSMDPVSIVTKKSTVLHKRDGNVIRTQIGDGADAFVSTITLPEQSPSVHS
                     SAEWVTANDYIYWGNGVCDRTFYNAGLANTKVSLVSSQHAAITDGTFWALFVEPDPVH
                     ILILNNA"
     gene            1193..>1631
                     /locus_tag="PKI33_14220"
     CDS             1193..>1631
                     /locus_tag="PKI33_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNM38221.1"
                     /translation="MSIAPLTTDDILRLWSQTYNREGKPDWSHIFPYYHDAIVFQDSI
                     QRVEGKENFIALCNRLTQRCEQLHMDISSIAQTKNEIFMEWKMTMIFKKFPSTPMFGC
                     TKLTFGEDNRIINQRDYYDLWGDIFNEIPWFHRPYRKFMHKYFG"
BASE COUNT          452 a          415 c          373 g          391 t
ORIGIN      
        1 tgcattattc agaatcaaaa tatgaaccgg gtccggctcc acgaacagcg cccaaaaggt
       61 cccatccgtg atggcagcgt gttgactgct gaccaagctc accttcgtgt tggctaagcc
      121 tgcattgtag aaggtacggt cacaaacccc gttgccccaa tagatatagt cattggcagt
      181 cacccactcc gccgaggaat gaaccgaagg cgattgctcc ggcaaggtga tggtggagac
      241 aaaagcatcg gcgccgtcgc cgatctgggt gcggatcacg ttcccatcgc gcttgtgcaa
      301 cactgtagat tttttcgtaa cgatgctgac cgggtccatg gaggttgtgg aggaacgcgc
      361 atccacgatc atataacgag ccgtgttcgt agaatcgcgc acgaacaccg accactccgc
      421 tcgaacgccg ctcaccaagc cagatacgcg atacgcattg agcgccagat aatacgatgg
      481 ctcatcgatg tcgagacagc gaacaggagt caacgagaga ttgggcggga tgcgcagctt
      541 ttgaatcagg ttctcaattt cagatgattt aatccggtac acccaataga ccgaaggcgg
      601 gtccatttca acggtgaact gtacatgcgg ttgatctttc ccaaacgcga tccgaagccc
      661 ggagtagatc gtcatttgag agtacataaa tcgtttcaga atgcttcgcc caagcctccc
      721 tagcttagac agtttttttg cgaatacggg gttctccatg aattgactcc aaaattggtc
      781 ttaatgtgta ggggcgaccc gtcagggtct agccaaaacc caaatcgata agaaagggtc
      841 gccctttgcc aaaaaaatta ccaagaacgc ggttgggtcg cgcctacggc gtaaggtgag
      901 ttaagaatta ttcctaagaa tgtcgttaat tatatttggc attccgacaa atatagtaca
      961 cttaaaaagt atagactatt acacattcgt gcgactttag atgcgtcgcc cagttaaatc
     1021 cataccttat attccgcgat ttcggcgtag aggcgacccg tcagggttta accgtatgcc
     1081 agcctacaaa aaagggtcgc cctttgcata aggtatccac aaaatgtggt tgggtcaccc
     1141 ctaccgcgta acatcaatat caaataattt gtccttccaa taaaaggagt ttatgagtat
     1201 cgccccccta accacagatg acatcttaag actttggtca cagacctaca atcgcgaggg
     1261 caagcccgat tggtcacata ttttccctta ttaccatgat gccatcgtgt ttcaagacag
     1321 catccaacgc gtggaaggca aggaaaattt cattgcctta tgcaatcggc tcacgcagcg
     1381 ctgcgaacaa ctccacatgg atatttcatc tattgcccaa acaaagaatg agatcttcat
     1441 ggaatggaag atgaccatga tcttcaagaa gtttcccagc acgcccatgt tcggctgcac
     1501 caagctcact tttggcgaag acaatcgcat catcaatcaa cgcgattact acgatctctg
     1561 gggcgatatc ttcaatgaaa tcccctggtt ccatcgtccc tacagaaaat tcatgcacaa
     1621 atattttgga t
//
LOCUS       DAOREF010000784         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67728_length_1630_cov_4.201270, whole genome shotgun sequence.
ACCESSION   DAOREF010000784 DAOREF010000000
VERSION     DAOREF010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67728_length_1630_cov_4.201270"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKI33_14225"
     CDS             complement(<1..547)
                     /locus_tag="PKI33_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38222.1"
                     /translation="MTIRPLDLLDLPLIARYRNDVLTLDSTRALTRGHPLGAMGLLAY
                     INPARHLYAAVSDNEGVTLLGGVIHTRGEPFAKLLYLAPSQQLDTPQLTALIEHLTAQ
                     AGEWQAFHVIAEVDETSDVFIALRKAGFSVYAWQRVWNASLIQKTESNPGTGWSRVKS
                     IDLPAIQRMHYQIVPQLLHLVD"
     gene            complement(583..1161)
                     /gene="pdxT"
                     /locus_tag="PKI33_14230"
     CDS             complement(583..1161)
                     /gene="pdxT"
                     /locus_tag="PKI33_14230"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNM38223.1"
                     /translation="MKIGVLALQGDFAEHVSMLKRVGVEAAEVRLPKHLDDLNGLIIP
                     GGESTTIGKLAVAYNLIDPLREFGKSHAIWGTCAGAIFLSNDIGRDQPLLGLMSIKVK
                     RNAFGRQVDSFETDLEIEELYKATKTKHPYHAVFIRAPIIESVSGDARVLSALEDGRI
                     IAAQQGHLLATSFHPELTDDTRFHEYFVSLAS"
     gene            complement(1209..>1630)
                     /locus_tag="PKI33_14235"
     CDS             complement(1209..>1630)
                     /locus_tag="PKI33_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNM38224.1"
                     /translation="DVVEAVRHARTVIGDIKKLTVMGDEELMAFAKNNGAPYELVKEV
                     KELGRLPVVNFAAGGVATPADAALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKSVTH
                     YQDASILAEVSKNLGEAMVGRNVSQMAEGEKIAGRGW"
BASE COUNT          408 a          460 c          386 g          376 t
ORIGIN      
        1 ggtcaacaag atgcaataac tgtgggacga tctgataatg catgcgttga atagctggca
       61 ggtctatgga cttaacccgg ctccatcccg tgccggggtt tgattctgtt ttctgaatca
      121 gactcgcatt ccaaaccctt tgccatgcat agacggagaa acctgccttg cgcaatgcaa
      181 tgaacacatc actagtctcg tccacttccg cgataacgtg aaatgcctgc cattccccag
      241 cctgtgcggt gagatgttcg atcaatgctg taagttgtgg ggtatcaagt tgctgtgaag
      301 gcgcgaggta caacaatttg gcaaaaggtt cgccacgggt gtgaatgact ccgcctaata
      361 aggtcacacc ttcgttatca ctgaccgccg catacaaatg ccgcgccggg ttgatatacg
      421 ccaacaatcc catcgcaccg aggggatgtc cgcgtgtgag ggcacgcgtg ctatcgagag
      481 tcaaaacatc gtttcgataa cgagcaatga ggggcaggtc taacaggtcg agtggacgaa
      541 tagtcatagt aggggcgcga aggttgcgcc cctacaaata tatcacgatg ctaatgagac
      601 aaaatactca tggaagcgcg tatcgtcggt caactcgggg tggaaggatg ttgccagcaa
      661 atgcccctgt tgtgctgcaa tgatgcgccc atcttccaag gcagaaagca ctctggcatc
      721 tccactcacc gattcaatga tcggcgcacg gatgaatacc gcatgataag gatgctttgt
      781 tttggtggct ttatacaact cttcgatctc aaggtcagtc tcgaacgaat cgacttgccg
      841 cccaaacgca ttgcgcttca ctttgatact catcaaaccg agcaaaggtt ggtcacgtcc
      901 aatatcattc gagagaaaga tggcacccgc gcaagttccc caaatcgcat gtgactttcc
      961 gaactcgcgc aacgggtcga tcaagttata cgccactgcc aatttgccaa tcgtcgtcga
     1021 ctcgccgcca gggatgatga gcccgttcaa atcatccaaa tgctttggca ggcgcacttc
     1081 cgcagcttct acaccgactc tcttcagcat agaaacatgt tctgcaaaat caccttgtaa
     1141 cgctaaaaca ccgattttca tatcattcaa tgtaggggcg gggtaacccc gcccctacca
     1201 aaataaattt accaaccgcg tccggcgatt ttttcgcctt ctgccatctg cgagacgtta
     1261 cgtccgacca tggcttcgcc gaggttcttg ctcacttccg caagaatgga agcatcttga
     1321 taatgcgtca ccgatttgac gatcgctgcc gcacgcttgg cagggtcacc agatttgaag
     1381 atgcccgagc caacaaagac gccgtccaca ccgagctgca tcatcaacgc ggcatcggca
     1441 ggggtcgcca cgccgcccgc agcaaagttc accaccggca aacgaccaag ttccttcact
     1501 tctttcacca attcatatgg cgcgccgttg ttcttggcaa acgccatcaa ttcttcatca
     1561 cccatcacgg tcaatttctt gatgtcaccg atcacagtac gagcatggcg cacggcttct
     1621 accacgtcac
//
LOCUS       DAOREF010000785         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_67961_length_1627_cov_3.853690, whole genome shotgun sequence.
ACCESSION   DAOREF010000785 DAOREF010000000
VERSION     DAOREF010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67961_length_1627_cov_3.853690"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1078
                     /locus_tag="PKI33_14240"
     CDS             170..1078
                     /locus_tag="PKI33_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38225.1"
                     /translation="MKRTIALTLSLLWLTACVTLTNPAPLIPTETVPTTPSVSAADNT
                     PTYVPLINNSALLYISGTTHIESKPQSWPNIDAFLNFLQNITAQGLKWSVGADIGWLE
                     GEPRAAELIQASEALGVQWDIHAHNPQERAKAAYLITQLGGHPTSVVSGMLVSEFSSI
                     GPQTYQGFTWTPHVLWGGTNCPGHRPGCDDLAAGIWIPLSAEQYTTHDPNGTLIRVGG
                     GSHQLADGLVLAESIANRQYPYPVISFTLMVSPTSLTIVNSNDSLEQITAFANDMKQY
                     PFVYWAYIEETAQAWTKAGKVPSRIE"
     gene            complement(1118..>1627)
                     /locus_tag="PKI33_14245"
     CDS             complement(1118..>1627)
                     /locus_tag="PKI33_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38226.1"
                     /translation="VILGIGILIPLPELLFKPLYQVQTPTETFSVLDETGLFEGSNFS
                     KEELTAFLAEPGAMVVTGRALYPRYYPAGEGESDLNTHYKKFHFQRLVFTVIGPDFPG
                     AIGLVIPGDSLPKGLSLQAADVVVLGCWTPDRYSPLLNAVVVFVTSGDGYVYNRSPES
                     PLHCPLKIP"
BASE COUNT          479 a          396 c          331 g          421 t
ORIGIN      
        1 catccatatt gtatttttta gggggcaatt ctacccctga actctacggt tttttacaat
       61 ctatttacaa ctctatgata gctgtcacga ttaattccat gcaagcatga tgcgagtcac
      121 tcttactaaa tttttcaaaa tcatagaatc taccttaaag gagaatatga tgaaacgaac
      181 gattgccctc actcttagtt tgttatggct cacagcatgt gtaactctga cgaatcctgc
      241 gcctcttatt ccaacagaaa ctgttccaac aaccccaagt gtctctgcag ccgacaatac
      301 tcccacatac gtgccgctta tcaataacag cgcattactc tatatttccg gcactactca
      361 cattgaatcc aagccgcaat catggccaaa tatagatgca tttctaaatt tccttcaaaa
      421 cattacagca caaggtttga aatggtcagt tggagcagat ataggctggc tagaaggaga
      481 accgcgtgct gctgaattga tccaagcctc tgaagcattg ggggtgcaat gggatatcca
      541 tgcccataac ccacaagagc gtgccaaagc agcttatctc atcactcaat taggcgggca
      601 ccctacttca gtcgtatctg ggatgcttgt cagtgaattc agtagcatcg gacctcaaac
      661 ataccaaggt ttcacatgga ctccacacgt gttatggggt ggcacgaact gtcccggtca
      721 ccgcccagga tgtgatgatc tagcggcagg catctggatt ccacttagtg ctgagcaata
      781 caccactcac gaccccaatg gcacactcat ccgcgttggc ggtggctctc atcaattagc
      841 cgatgggcta gttctcgccg aatctatcgc aaatagacaa tatccatatc cagttatcag
      901 tttcacatta atggtctctc ccacctctct aaccatagtc aacagcaatg atagcttaga
      961 acaaatcaca gcatttgcaa acgatatgaa gcagtatccc tttgtatatt gggcatatat
     1021 tgaagagaca gctcaagctt ggactaaagc cggaaaggtt cccagtcgaa ttgagtgaaa
     1081 taaaaacagg tcacgtctta taacgtgacc tgttttatca aggtattttt aatggacagt
     1141 gcaacggtga ctctggtgaa cggttgtata catatccatc gccagaggta acaaaaacca
     1201 caactgcatt caggagcggg gagtatcgat ccggtgtcca gcatccaagc acaaccacat
     1261 ctgccgcttg tagtgaaagc ccttttggta gagagtcacc cggaatcact agtccaattg
     1321 caccaggaaa gtccggtccg atcacggtaa acactaatct ttgaaaatga aattttttgt
     1381 aatgtgtatt taagtcagac tcaccttcac ccgccggata gtaacgcgga tataaagccc
     1441 gtccagtcac gaccatagcg cctggctcag caagaaaagc ggtcaactct tcctttgaga
     1501 aattggaacc ttcgaaaagc cccgtttcat ccaaaacaga aaaggtctcg gtaggagttt
     1561 gtacttgata gagtggttta aataagagtt ctggcaatgg aatcaaaatg ccaattccta
     1621 agattac
//
LOCUS       DAOREF010000786         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68179_length_1625_cov_2.971338, whole genome shotgun sequence.
ACCESSION   DAOREF010000786 DAOREF010000000
VERSION     DAOREF010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68179_length_1625_cov_2.971338"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..726
                     /locus_tag="PKI33_14250"
     CDS             145..726
                     /locus_tag="PKI33_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNM38227.1"
                     /translation="MSTSRQKILTHLKKTRSASAREIARALKLSAPNVRHHLSVLCSD
                     GRVEMISLHNREGRGRPEKVYSLSAAALGDNLSALAEAILTVASKTLSMEAVASRILD
                     AKQFAGQPINKRLALLVEALNEMHYQARWEAGAGGPRVIFGRCPYARVIDGHPEICKM
                     DEAMLKAALEREILPTRRAETQQGLCPFLFEIG"
     gene            730..1257
                     /locus_tag="PKI33_14255"
     CDS             730..1257
                     /locus_tag="PKI33_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38228.1"
                     /translation="MFYEFTEEELRSNQRGFISPKQKEFLNRYGKSLIQSHRSGWPVI
                     IFFMVLGGGIILGMNLSNESTRRALFADPLNLVILCASIPLILGVYGLGAFWARHTAS
                     RFREPELRVAEGAIRWDDEYSDSVGRTYFLYVGKTEFKFAEDLSYTFPIGKRARIFYC
                     EGSQIKLILSHELLS"
     gene            complement(1262..>1625)
                     /locus_tag="PKI33_14260"
     CDS             complement(1262..>1625)
                     /locus_tag="PKI33_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNM38229.1"
                     /translation="RAFVGCVLVREKRILTTGFNGSPAGQDHCDEIGHLMVDGHCVRT
                     IHAETNAIIQAALHGVSTKGATCYVTHLPCINCTKALINAGIARIVYSVAYRSDENAL
                     NFLKAADIEVAQREFTPS"
BASE COUNT          407 a          379 c          398 g          441 t
ORIGIN      
        1 ttgaattatg caaaaatata tttacataaa taaagaagtg aatataatca ctccatcagg
       61 atgacaccct cgcttcgccc ttaacttgaa cctgtctctg tttctttgaa ctttccctac
      121 tctgaaaact tgcgtataga caatatgtcc acttcccgcc aaaaaatcct cacacatctc
      181 aaaaagactc gctctgcttc tgcccgtgaa attgcacgcg ccttgaaact ctccgcgccg
      241 aatgtgcgcc accacctctc tgtgctttgc tctgatgggc gcgtggaaat gatcagcctc
      301 cacaaccgcg aggggagggg cagaccggag aaagtctatt cgctttcggc agcggcgttg
      361 ggagataatc tctccgcgtt ggcagaggct attttgacag tagccagcaa aacgttaagt
      421 atggaagcgg tggcgagtcg cattttggat gcaaagcaat ttgcaggtca acctatcaat
      481 aaacgtttgg ctttgttagt cgaagcgttg aatgagatgc attatcaagc ccgttgggag
      541 gcgggtgctg gaggtccgcg cgtgatattt gggcgttgcc cgtacgcacg ggtgattgat
      601 ggacaccccg agatctgtaa aatggatgaa gctatgttga aagctgcact ggaacgagag
      661 attttgccca ctcgccgtgc agagacacaa caaggtttgt gcccattttt atttgagatc
      721 ggatgaatta tgttttacga atttacagaa gaagaattac gctcgaatca gcgtggtttt
      781 atttccccaa agcaaaaaga attcttgaac cgatatggaa aaagcttaat ccagtctcac
      841 cgcagcggct ggcccgtgat tattttcttt atggtgttgg gcggcggaat tattcttggc
      901 atgaatcttt ccaatgaaag tacccgccgg gcgctctttg ccgaccctct caacctagtt
      961 attttgtgtg catctatacc tttgatttta ggtgtgtatg gcttgggcgc cttttgggcg
     1021 aggcatactg cttctcgctt tcgtgaacca gagctgcgtg tggcagaagg tgcaatccgc
     1081 tgggatgatg aatattccga ctcagtcggg cggacttact ttttatacgt tggaaagact
     1141 gagtttaaat ttgcagaaga cctgagctac acatttccaa tcggcaaacg cgcacggatc
     1201 ttttattgcg aagggtctca gattaagctg attctttcgc atgagctatt atcttagaaa
     1261 attaactggg tgtaaattct ctttgcgcaa cctcaatatc agcggctttt agaaagttca
     1321 acgcattctc atcgctgcga tatgccacac tatatacaat gcgggcgatg ccagcgttga
     1381 tcaacgcctt ggtgcagttg atgcagggta aatgggtgac gtaacaagtt gcgcctttgg
     1441 tggaaacccc atgcaacgct gcctgaataa tcgcatttgt ttcagcatga atcgtgcgga
     1501 cgcagtgtcc atcgaccatc aggtgcccaa tttcatcgca atgatcttgc ccagcggggg
     1561 agccgttgaa cccagtcgtt agaatccgct tttcacgcac aaggacgcat cccacaaagg
     1621 cacgg
//
LOCUS       DAOREF010000787         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68342_length_1623_cov_2.272321, whole genome shotgun sequence.
ACCESSION   DAOREF010000787 DAOREF010000000
VERSION     DAOREF010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68342_length_1623_cov_2.272321"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PKI33_14265"
     CDS             complement(<1..881)
                     /locus_tag="PKI33_14265"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM38230.1"
                     /translation="MKEQHPAIHPELQGPAKFAPKLNYNKFVVWLLNTLMRLAPSPKA
                     PTDLHIENIFITASDGNQVRVRVYKPKAAKEALPALLWLHGGGYLIGNPEQDENTCIQ
                     YVRDLGIQIVSVDYRLAPKHPFPAGLDDSYTALKWMATQANELGIDNKRIAIGGASAG
                     GGLAAALVQFAHDKQEVQPVFQLLVYPMLDDRTALRTDLHDINIAWNQPSNRFGWSSY
                     LGKECGSDSVSEYSVPARRENLAGLPSAWVGVGDLDLFYTEDLLYAQHLQAAGVDCEI
                     VVVPGAFHGFDAFTSQAS"
     gene            complement(916..1401)
                     /locus_tag="PKI33_14270"
     CDS             complement(916..1401)
                     /locus_tag="PKI33_14270"
                     /inference="COORDINATES: protein motif:HMM:NF040504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNM38231.1"
                     /translation="MIRVAKTEDAVKIAEIYNHYIANTVITFEKEPVTADEISTRMTD
                     VFSSSLPWLVLENNDEVIGYAYATKWRARPAYKNSVETTIYLAKDSGGKGFGKLLYST
                     LISTLTTSGFHTAIGGIALPNEASVGLHESLGFKKVAHFHEVGYKFDTWIDVGFWQIM
                     L"
     gene            complement(1426..>1623)
                     /locus_tag="PKI33_14275"
     CDS             complement(1426..>1623)
                     /locus_tag="PKI33_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38232.1"
                     /translation="VAAHELGAAAYAIKAVQAAALEGESKEAGRLECQWQRAQLPSQI
                     RALVLDDQKLRNEICWFVFDC"
BASE COUNT          426 a          409 c          359 g          429 t
ORIGIN      
        1 gaagcttgtg aagtgaacgc atcgaaacca tgaaatgcac ccggcaccac aacgatttca
       61 caatcaacac cggctgcttg taaatgttgg gcgtagagca aatcttcagt gtagaaaagg
      121 tccaagtcgc ctaccccaac ccaggcactc ggaagacccg cgagattttc acgtcgggct
      181 ggcacagaat actctgacac actgtcactt ccgcattctt tgccaagata ggaagaccag
      241 ccgaatcgat tgctcggttg attccaagcg atattaatgt catgtaaatc tgtgcgaagc
      301 gcggttctat catccaacat ggggtacacc aaaagctgaa agaccggttg cacttcttgc
      361 ttatcgtgcg caaactgcac caatgctgca gctaacccac cgcccgcact cgcaccacca
      421 atggcgatgc gcttgttatc gataccaagt tcatttgctt gagttgccat ccactttagc
      481 gcggtgtaac tatcatccaa accggcgggg aagggatgct tgggagcaag ccgataatcg
      541 acagacacaa tttggattcc cagatcgcgt acgtattgga tacaggtatt ttcatcctgc
      601 tcggggttgc caatgagata cccgccccca tgcagccata acaaagcggg taacgcctct
      661 ttcgccgcct tcggcttgta tacccgtacc cgaacttgat tgccatctga agctgtgatg
      721 aagatatttt caatatgcaa atctgtaggt gctttgggcg acggtgccaa acgcataagg
      781 gtattcaaca accaaaccac aaacttgttg tagtttaact ttggcgcaaa ctttgccgga
      841 ccttgtaatt cagggtgaat agctgggtgt tgttctttca tataaggtta tctgtttact
      901 ttagaaaaaa gaagctcata acatgatctg ccaaaagcct acatcgatcc atgtatcgaa
      961 tttgtatcca acttcatgga agtgagccac ttttttgaac cccaaggact catgcagacc
     1021 aacactagct tcattcggca aagcgatgcc tccaattgca gtgtggaatc ctgaagtggt
     1081 gagagttgag atcaaggttg agtagagtaa cttgccaaat ccttttccgc ctgaatcttt
     1141 ggctagataa atcgtcgttt cgacagagtt tttataggca ggtcttgcac gccattttgt
     1201 ggcatatgca tacccgatca cttcatcatt attttccagt acgagccacg gcaaagaaga
     1261 agaaaataca tctgtcatac gagtactaat ttcatcggca gtaacgggtt ctttttcaaa
     1321 tgtgatgacc gtattcgcaa tgtaatggtt gtagatctcg gcgattttga cagcatcttc
     1381 tgtctttgct actcgaatca tcctgcgctc ctgcaataaa cgttttcagc aatcaaacac
     1441 gaaccaacaa atttcattac gcagtttctg gtcatccagc accaatgcgc ggatttgact
     1501 cggaagttgc gctcgttgcc actgacattc caatcgcccc gcttcttttg attcaccttc
     1561 caatgccgct gcttggacgg ctttgatggc ataggcggcg gctcccagct catgggcagc
     1621 tac
//
LOCUS       DAOREF010000788         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68520_length_1621_cov_2.121328, whole genome shotgun sequence.
ACCESSION   DAOREF010000788 DAOREF010000000
VERSION     DAOREF010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68520_length_1621_cov_2.121328"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1621
                     /locus_tag="PKI33_14280"
     CDS             <1..>1621
                     /locus_tag="PKI33_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNM38233.1"
                     /translation="VYVPLFVGISQEGDGYPTDQILNGLTPLPSEMAIGATWNTEFAQ
                     NVGEVRGKEIAALGFNIFLGPSLDVLETPSSSGGDLGTRVFGGDPFWVGEMGRAYING
                     LHTGSNGNMLVIAKHFPGAGGADRLPQEEVATVRKSLEQLKQVEFAPFFAVTGNATLP
                     SETVDGLLVSHIRYQGFQGNIRATTRPISFDQQALGQIMALPELAGWYSNGGLLVSDN
                     LGTTAVHEFYAAGGGQFQARIAARDSFIAGNDLLYLGNIKSDDTPDSYTSVLRTIDFF
                     AQKYREDPAFAQRVDASVARILAAKFKLYENFDIGNVTVPAVELDQSDAGSNITFQVA
                     RNSATLISPSQQDLASILPLPPQPSDRIVFLTDSLSVAQCTDCTEQPLLATDALQNVV
                     LRLYGPQSGNQVEEFRLKSYSFDNVKAWLDAPDENQFVADDLDNADWVVISITDSSRE
                     QPALISRLFRERPTLLLNKQLILFSFGAPYYFDTTTLSRFTAYYALYSKQPQFVDVAV
                     RLLFQEVTPAGASPVSIPAVGYDLIDVTSPSP"
BASE COUNT          391 a          357 c          419 g          454 t
ORIGIN      
        1 gtctatgttc cgttatttgt tggtatttca caggaagggg atgggtaccc aaccgaccaa
       61 attctgaatg ggttaacccc tttacccagt gaaatggcta ttggcgcaac atggaacacg
      121 gaatttgcgc aaaatgttgg agaagttcgc ggcaaggaaa ttgctgcctt aggttttaat
      181 attttccttg gtccatcgtt ggatgtgtta gagactccat cttcatccgg cggtgatttg
      241 ggaacgcgtg tgtttggcgg tgacccgttt tgggtgggcg aaatggggcg agcctacatt
      301 aatgggttgc atacgggctc caatggaaat atgttggtga tcgccaagca ctttccgggg
      361 gcaggcggag cagatcgttt accgcaagaa gaagtggcta cggttcgaaa atctcttgag
      421 caattgaaac aggtggagtt tgcacctttc tttgctgtaa caggaaatgc aacactcccg
      481 tcagaaactg tcgatgggtt gttggtttcc cacattcgtt atcaagggtt tcaaggaaac
      541 attcgcgcaa ccacacgccc gatcagtttt gaccagcagg cgttggggca gatcatggca
      601 ttgccagagt tagcagggtg gtacagtaat ggcggactat tagtaagtga caatcttgga
      661 acgacggctg tgcatgaatt ctatgccgca ggcggaggtc agtttcaggc acgtattgca
      721 gcccgcgatt ctttcattgc gggaaatgac ttactctacc taggcaatat taagagtgat
      781 gatacgccag atagttatac atctgtgctt cgtacaattg atttctttgc acagaaatat
      841 cgtgaagacc ctgcatttgc acagcgagtg gatgcttcgg ttgcgcgcat tcttgcggca
      901 aaatttaaac tctatgagaa ttttgatatc ggaaatgtaa ccgtccctgc ggttgagtta
      961 gaccagtcag atgcgggaag taatattacg tttcaagtgg cgcggaactc tgctacattg
     1021 attagcccca gccagcaaga tctggcttcg atcttaccgc tgccgccgca acccagcgat
     1081 agaattgtat tccttaccga ttctctcagt gtagcgcaat gtacagattg tacagagcaa
     1141 ccacttcttg cgacagatgc gctgcagaat gttgttttgc gtttatatgg tccgcagagc
     1201 ggaaatcagg tggaagagtt caggcttaag tcttattcat ttgataatgt aaaagcctgg
     1261 ttggatgccc ccgatgaaaa tcaatttgtg gcagatgatc tggataatgc ggattgggtc
     1321 gtgatttcca ttaccgactc atctcgtgag caaccagcat tgatcagccg cctctttcgc
     1381 gaacgtccga ccttgctgct gaacaaacag ttgatcttgt tctcgtttgg cgcaccgtac
     1441 tattttgata ccactacgct ttcacggttt actgcctatt atgcgttgta tagcaaacag
     1501 ccccaatttg tggatgtggc agtacgcttg ttattccaag aggtaacacc cgctggcgct
     1561 tcaccggttt caattcctgc ggttggatat gatttgatcg atgtcacatc gcccagcccg
     1621 a
//
LOCUS       DAOREF010000789         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68533_length_1621_cov_1.796935, whole genome shotgun sequence.
ACCESSION   DAOREF010000789 DAOREF010000000
VERSION     DAOREF010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68533_length_1621_cov_1.796935"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="PKI33_14285"
     CDS             <1..1469
                     /locus_tag="PKI33_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM38234.1"
                     /translation="GYTKKVMSKQDLILLALDASPTLDLMVRAFHSANYEVAVVHDQS
                     GLEKSIQQAIPALMIIGEIFNGEDGFSIATNTLERFPTMPIILYAEKDKPGLAQHIIK
                     AGISGYLYPPLKLDDIVEEVKRTLIRARKLGDWLRREVKRTTASLEQKAQISEAERNK
                     LDAIISNIQDGVLVLDETHHILLANKTIYEIFSLNGNPILGKYVKDVINSADLNALID
                     RARNGALKYHEINFDDGRVYNAQYTPLPRIGSAVTMQDISYLKEIDRLKNDFVHTVSH
                     DLRSPLTSVLGYMELIERVGPLNESQHEFLERLRAGVHHMTSLVNELLDLGRLESGFD
                     TRRETVNLKNVLEYSLNVFDNQIKKKNLQVTYEFADNLKSLRANPIRIRQMIDNLVGN
                     AIKYTPPNGAVKVSLGMQENQVILRVTDSGPGIPPDEQNRVFEKFFRASNRPSETEGS
                     GLGLAIVKSIVESHQGRVWVESKVGVGSTFIVLLPAQE"
BASE COUNT          490 a          350 c          357 g          424 t
ORIGIN      
        1 aaggatatac aaagaaagta atgtcaaaac aagacctgat tttgctggca ttggatgcat
       61 cgcccacgtt ggatttgatg gtgcgtgctt tccactccgc aaattatgaa gtagcggtag
      121 tccacgacca gtcaggatta gagaaatcaa tccaacaagc cattccagca ttgatgatca
      181 ttggggaaat tttcaatgga gaagatggct tttcaattgc cacaaacaca ctagaacgtt
      241 ttcccaccat gcctatcatt ctctatgcag agaaagataa gcccgggctg gctcaacata
      301 tcatcaaagc aggcattagt ggatatttat atccgcctct aaagctggat gatattgttg
      361 aagaagtaaa gcgcactctt atccgcgccc gaaagctggg agattggctt cgccgagagg
      421 taaagcgcac aacggcttcg ctggaacaaa aggctcaaat ctcagaagca gaacgaaaca
      481 agttagatgc gatcatatcc aatattcaag atggtgtatt agtcttggat gaaacgcatc
      541 atattctgtt ggcaaacaaa accatctacg aaatctttag tctcaacggg aatcccatcc
      601 ttggaaagta tgtcaaagat gtgattaaca gcgcagactt aaacgctctg attgaccgtg
      661 ctcgaaatgg cgcgttgaaa tatcatgaaa tcaactttga cgatggacgc gtatacaacg
      721 cacaatacac tcctctgcca cgcatcggca gtgcagtgac catgcaagat atttcatact
      781 taaaagaaat tgaccgtttg aagaacgatt ttgttcatac cgtttctcac gatttgcgat
      841 ccccactgac ctctgttttg ggatacatgg aactaattga acgcgtgggt ccgctgaacg
      901 agtcgcagca tgagtttcta gaacgtttac gagcgggtgt acatcacatg accagtttgg
      961 tcaatgaatt actcgacctt gggcggcttg aatcaggctt tgatactcgt cgcgaaactg
     1021 tcaatctcaa gaatgtgctt gaatactctt tgaacgtctt cgacaaccag atcaaaaaga
     1081 agaatcttca ggttacatac gaatttgcag ataatctcaa atcactgcgc gcgaatccta
     1141 ttcgcattcg tcagatgatc gacaatttag taggtaatgc tattaagtac acaccgccta
     1201 atggagcggt aaaagttagc cttggtatgc aagaaaatca ggtcattttg cgggtcacgg
     1261 atagtggacc aggcattccg ccagatgaac aaaaccgtgt attcgaaaag ttcttccgcg
     1321 cctcgaatcg ccccagcgaa acggaaggct ctgggctggg gttggcaatt gtcaaatcaa
     1381 ttgttgagag ccaccaaggg cgtgtgtggg tagaatcaaa agtgggtgtt ggctccacat
     1441 tcatcgttct tcttccggca caagagtaaa aataataaaa acaaaataga aggtgtacat
     1501 ggaccttcta ttttgtttta agaacgtatt ttttacagtt tggtcactac ttctgaatta
     1561 tcctgatact tcaataaacg caatccttca agccgcaacg cttctgggaa caatgcttta
     1621 t
//
LOCUS       DAOREF010000790         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68556_length_1620_cov_3.642173, whole genome shotgun sequence.
ACCESSION   DAOREF010000790 DAOREF010000000
VERSION     DAOREF010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68556_length_1620_cov_3.642173"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKI33_14290"
     CDS             <1..529
                     /locus_tag="PKI33_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM38235.1"
                     /translation="DQVTAFCNNANSAICKRLWDLHPYDETLTGLEDLEWSKWAKAQG
                     HKIAYIAEAEIVHIHNETPKGVYNRYRREAMALRRIYPESNFNLYDFLRLTITNILSD
                     LWHAARERVFLKSVPSIFWFRFAQFRGTYMGHRETSLVTPQLRETFYYARERKKKDER
                     KRDVEPIQYNKGNDE"
     gene            567..1151
                     /locus_tag="PKI33_14295"
     CDS             567..1151
                     /locus_tag="PKI33_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNM38236.1"
                     /translation="MATPPPNSVHPKSRAEWRKWLEENQARKEGIWLISYKKDTGKPR
                     FDYDESVEEALCFGWVDSKPAKLDEERSMLWFAPRKPKTNWSKLNKDRVEKMIKAGLM
                     APAGLAKVEAAKKDGSWNALDGVEALEIPPDLAKALSKNKTAKEYFEAFPRSVKRGIL
                     EWILNAKRPETRAKRIEETVTLAEKNIRANQWRQ"
     gene            1191..>1620
                     /locus_tag="PKI33_14300"
     CDS             1191..>1620
                     /locus_tag="PKI33_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM38237.1"
                     /translation="MPTLSLKPASEYTIPFLADLMTRGFEGYIVPINITDNVLHTMIR
                     RDGIDLTSSRVLVRDEEPIGVAFIARRGWTSRLAAFGITSNARNGGVGTWAMEQLLQE
                     ARERGEKEMLLEVIEQNTAGVKLYERVGFTKIRRLVGYKLE"
BASE COUNT          476 a          392 c          400 g          352 t
ORIGIN      
        1 ggatcaagtc acggcgtttt gtaataatgc caactccgcc atttgcaaac gtttatggga
       61 cttgcatccc tacgacgaaa cgctgaccgg cttggaagat ttggaatgga gcaagtgggc
      121 aaaagcgcag ggacataaaa ttgcctacat tgccgaagcc gagatcgtcc acatccacaa
      181 tgagacgccc aaaggggttt acaaccgcta tcgccgtgaa gccatggcgt tgcgacgtat
      241 ctatcctgag tcgaatttca atttatatga tttcctgcgc ttgaccatca cgaatattct
      301 gagtgatctg tggcatgccg cccgcgagag agtttttctg aaaagcgttc cgtctatctt
      361 ctggtttcgc tttgctcaat ttcgaggaac atatatggga caccgcgaga cgagtctagt
      421 cacgccgcag ttaagagaga ctttttatta tgctcgggaa cgaaagaaga aagatgaaag
      481 aaaacgggac gtagaaccga tacaatacaa caaagggaat gatgaatagg taatagaccg
      541 ttgaaaaaga ctgtctatta tgtactatgg ctactcctcc ccccaactcc gttcacccta
      601 aatcacgagc agaatggcgc aagtggctgg aagagaacca ggcgcgcaaa gaaggtattt
      661 ggctaatcag ctacaagaaa gataccggca agccgcgctt cgactatgac gaatccgtgg
      721 aagaagccct gtgctttggc tgggtagata gcaaacctgc caaacttgac gaagaacgct
      781 ccatgttgtg gtttgccccg cgcaagccca aaacgaattg gtcgaagctc aataaagacc
      841 gcgtggaaaa aatgatcaaa gccgggctca tggctcctgc cggtctggct aaagtggaag
      901 ccgccaaaaa agacggctct tggaacgccc tcgacggtgt ggaagcactt gaaattccac
      961 ccgaccttgc caaagccctc tctaaaaata aaacagccaa agaatacttt gaagcattcc
     1021 cgcgctcggt caaacgcggc atcctggaat ggatactcaa cgccaaacga ccggagacac
     1081 gggcaaaacg aattgaagag acagtgacgc tagcagagaa aaatatccgc gcaaatcagt
     1141 ggcggcagta agtccaaaga catataagga tattcgatat aatttccact atgcctaccc
     1201 tttctctaaa acctgcttct gaatacacca tccccttcct cgcagacttg atgactcgcg
     1261 gatttgaagg gtacatcgtg cctatcaaca ttacagataa tgtattgcac accatgattc
     1321 gccgagatgg cattgacctg acatctagcc gtgtgcttgt tcgcgatgag gagccgatag
     1381 gcgttgcatt tatcgcccgc cgcggctgga cttctcgcct cgccgcattt ggcattacgt
     1441 ccaatgctcg taatggcggt gtaggaacat gggcaatgga gcaacttcta caagaagcta
     1501 gggaacgcgg cgaaaaggaa atgcttctag aggtaatcga acagaatacc gcaggcgtga
     1561 aactgtatga aagagtaggc tttactaaaa tccgcaggct ggtgggctat aaactcgaaa
//
LOCUS       DAOREF010000791         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68639_length_1619_cov_3.154731, whole genome shotgun sequence.
ACCESSION   DAOREF010000791 DAOREF010000000
VERSION     DAOREF010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68639_length_1619_cov_3.154731"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PKI33_14305"
     CDS             complement(<1..1211)
                     /locus_tag="PKI33_14305"
                     /inference="COORDINATES: protein motif:HMM:NF021493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNM38238.1"
                     /translation="MVNMKKYKAKNKGQVIVFMALVMVGLIAIVGLMTDGGMLLIEYG
                     KLKRAVDAAAISASQQFRRGFTGADLATAAQNFLRLNSSEASNINVYRCKRDPNTNAL
                     DTTADGTINADDLCTLPRRKLVRVEATRTVQFGFMRVLGIESSNITTSAVGEAASIDL
                     VLIIDTSGSMSYETYTSGDDNRLDHSSEDPAVCNGNVTTPCEPLNTVKNVADDFMDNM
                     FFPYDRVAVIAMTNQQPNALTRDPADSVLHLSDNEASVRAAIAGLRVFEPPVCDFNTR
                     TPASGPCISHASSVPALECFKYRDFGDVSSCNNSNVGGTFYRAAAEYSFGLIREDSLW
                     VAVALFGGPANATDAQTGYPDGLCPSYTWNSSVHPYCRDAYVSTYHSKTDPLYDADDY
                     ARDAAYYLADPV"
     gene            complement(1226..>1619)
                     /locus_tag="PKI33_14310"
     CDS             complement(1226..>1619)
                     /locus_tag="PKI33_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38239.1"
                     /translation="PFYANTVAMVRANLDPQVVNPSYQGRRISLDPLKDDVIVTAYAT
                     SAGHVVSSYPAAGPYHLYGNASPIFATNAAIENKLVSGAPNAGLLLVEVHYNYHQVLR
                     LPWLLVFVPDPIVLRAYTIMPLAAAEPP"
BASE COUNT          459 a          360 c          370 g          430 t
ORIGIN      
        1 acagggtcag caagatagta ggctgcatct cttgcataat catctgcatc atacaaagga
       61 tcggttttag agtggtatgt gcttacatag gcatcacgac aatatgggtg gactgaggaa
      121 ttccaggtgt aacttgggca gagaccatcg gggtaacctg tttgtgcatc tgttgcattt
      181 gcaggaccgc cgaataaagc aactgcaacc caaagggaat cttctcgaat taagccaaaa
      241 gaatattctg cagcagcacg ataaaacgtt ccgccaacgt tactgttatt gcacgatgac
      301 acatcgccaa aatcgcggta cttaaaacat tcgagcgccg ggactgagct tgcgtgacta
      361 atacaaggac cagatgctgg tgttctagtg ttaaaatcac atactggtgg ctcaaacacg
      421 cgcaatcctg ctatagcagc gcgtacactg gcttcgttat ccgataggtg gagaacagaa
      481 tccgcaggat ctcgggtgag cgcgttaggc tgttggttcg tcatggcaat tactgctacg
      541 cggtcatacg ggaagaacat attatccatg aaatcatctg ctacattttt cactgtattg
      601 agcggttcac atggtgtggt gacattgcca ttacaaacag ctggatcttc gctgctatgg
      661 tcaaggcgat tgtcatctcc agatgtgtat gtctcgtatg acatagaacc agatgtatca
      721 ataattaaga cgaggtcaat tgaagctgct tcacccacag cagaagtagt aatattactt
      781 gactcaatcc caagcacacg cataaaaccg aactgaacag tgcgggtcgc ctctacccgc
      841 acaagctttc tgcgagggag agtgcaaaga tcatcagcat ttatagtgcc atccgcggtg
      901 gtatctagtg catttgtatt tggatctctt ttgcagcgat atacattaat attcgatgcc
      961 tcggaactat ttaaacgtaa aaagttttgc gcggctgtcg caagatcggc gcccgtaaat
     1021 ccacggcgaa attgttgtga agctgaaatt gccgctgcat ctactgcgcg ttttaactta
     1081 ccatattcga tcaaaagcat acccccatct gtcattaaac caacaatggc tatgagccct
     1141 accataacca atgccataaa tacaatgact tgacctttat tttttgcctt atattttttc
     1201 atattcacca tacaagtctc aatccttatg gaggttcagc ggcagctaat ggcataatcg
     1261 tatatgctcg taaaacgata ggatctggaa caaatacaag aagccagggt aaacgtaata
     1321 cttggtgata gttataatga acttctacca ataaaagccc ggcattgggc gcaccactga
     1381 caagcttatt ttcaatcgca gcatttgttg caaagatggg gctggcgttc ccgtatagat
     1441 ggtaaggtcc agcagctgga taactgctga ccacatgccc tgcagatgtt gcataagcag
     1501 taacgattac atcatcttta agtggatcga gagatatccg gcgtccttga tatgaggggt
     1561 tgaccacttg tggatcaagg ttcgcacgta ccattgctac tgtgttagca tagaatggc
//
LOCUS       DAOREF010000792         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68874_length_1616_cov_4.190903, whole genome shotgun sequence.
ACCESSION   DAOREF010000792 DAOREF010000000
VERSION     DAOREF010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68874_length_1616_cov_4.190903"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKI33_14315"
     CDS             complement(<1..336)
                     /locus_tag="PKI33_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNM38240.1"
                     /translation="MRDIALSLSLAGLAFIMTAIWGNPLLRILRHFKLGKIIRIEQPE
                     GHRVKMGTPTMGGVMFIVPVLLLSGLLNAVALIGVTASGVGRSVLLPMVVMVGFAILG
                     AVDDWEGIHG"
     gene            complement(333..>1616)
                     /gene="murF"
                     /locus_tag="PKI33_14320"
     CDS             complement(333..>1616)
                     /gene="murF"
                     /locus_tag="PKI33_14320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNM38241.1"
                     /translation="KTDGHDYLTDAFKRGASFALIQRDVDASFRTLDLRAGLSTDATL
                     NFESPLCLRVDNTISALQQIARFWRRKLPNLRVVGITGSVGKSTTKETVAEVLSLRYR
                     TLKSPGNLNNEIGLPLTILRLGTGYDRAVLEMGFYVPGEIQFLCDIAQPQMGVVSNVG
                     TVHAERAGSQEAIFRGKSELPASLPADGVAILNFDDQWVRKMEEKTKARVFFYGLSPE
                     AHLWADQIEGLGLDGIRFRMHYGKEIIHMRVPMIGRHSIHTALRAAAVGLNDGISWQE
                     ILEGLSHGHTQLRLVAARNETGALILDDTYNAAPESMLAALNLLDELDGRKIAVLGDM
                     LELGPYERQGHEMVGMRAAQVAKVLITLGTRGHMIAEAARRAGMKASAIMEYEDIAPI
                     IDWLTKNLNSNDAVLIKGSHGLRMDRIAAALEVRS"
BASE COUNT          440 a          445 c          358 g          373 t
ORIGIN      
        1 gccgtgaata ccttcccaat catccaccgc acctaaaatg gcaaagccaa ccatcactac
       61 catgggcaac aacacagagc gacccactcc gctggcagtt acaccaatca aggcaacggc
      121 gtttagcaaa ccgctaagaa gcaagaccgg cacaataaac atcaccccac ccatggttgg
      181 ggtgcccatc ttcacccgat gaccttccgg ctgttcaata cggatgatct tccccaactt
      241 aaagtgccgc aaaatacgca gcaacggatt tccccaaatg gcagtcatga taaaagccaa
      301 ccccgccagg cttagggaaa gagcaatgtc tctcacgagc gtacctccaa tgcagcagcg
      361 atgcggtcca tgcgtaagcc atgggatcct ttaattaaca ctgcgtcatt tgaattcaaa
      421 ttctttgtca accaatctat gatcggggca atgtcttcgt attccataat agcagaagcc
      481 ttcataccgg ctcgacgagc cgcttccgca atcatgtgtc cacgggtgcc caaggtaatg
      541 aggactttcg ccacttgagc cgcacgcatt ccgaccattt catgtccctg gcgttcgtag
      601 ggaccaagtt caagcatatc acctaacacg gcaatcttgc gaccgtctaa ttcgtccaac
      661 agatttaacg ccgccaacat agactcaggt gctgcgttat atgtatcatc caatatcaaa
      721 gcaccagtct cattgcgggc ggctacgaga cgcaattgag tatgcccgtg tgagagacct
      781 tctaaaattt cctgccaaga aatgccatca ttcaacccaa cagcagcagc acgtaatgcc
      841 gtatgaatgg aatgacgtcc gatcatcggc acgcgcatgt ggataatctc tttaccgtaa
      901 tgcatacgga agcggatgcc atccaaaccg agcccttcga tctggtctgc ccacaaatgt
      961 gcttctggag atagcccata gaagaaaacg cgagccttgg tcttctcttc cattttgcgg
     1021 acccactggt catcaaaatt tagaatcgca accccatcag ctggaagaga ggctggtaat
     1081 tcgctcttgc cgcggaaaat agcttcctgt gacccggcgc gttccgcatg gacagtacct
     1141 acatttgaaa caacacccat ttgcggctga gcaatatcac aaagaaattg aatctcgcca
     1201 ggtacgtaaa atcccatttc aagcacagca cggtcataac ctgtaccgag tcttaatatg
     1261 gtgagcggaa gtccaatctc gttatttaaa ttgccagggc ttttgagtgt gcggtaacga
     1321 aggcttaaca cctctgctac agtttctttg gttgtagatt tacccacact tcctgtgatt
     1381 cctaccacgc gcaggttcgg caatttacgg cgccagaaac gcgcgatttg ttgtaaggct
     1441 gaaattgtat tgtccacgcg caggcagaga ggagactcaa agttcagtgt ggcatcagtt
     1501 gatagaccag cgcgcaggtc gagggtccgg aaagaagcgt ctacatctcg ttggattaaa
     1561 gcaaaagaag cccccctctt gaaggcatct gtcagatagt catgtccatc tgtttt
//
LOCUS       DAOREF010000793         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_68886_length_1616_cov_3.199231, whole genome shotgun sequence.
ACCESSION   DAOREF010000793 DAOREF010000000
VERSION     DAOREF010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68886_length_1616_cov_3.199231"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..659
                     /locus_tag="PKI33_14325"
     CDS             168..659
                     /locus_tag="PKI33_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38242.1"
                     /translation="MKKYIGLFLFCVFIVSCVPVSEKLSQQDCKYLLANIKDENINTD
                     FRIIDVSNEKIYEDLYNLIVNVDNFSLSLLTLSPNKDLKVFFLQDSIWVQQVNSIEFP
                     LEISQIGVKSPDDPGGKTYSFMYNPANLEGTEKICVTMIAIKDPQGLSEPIGSYFEFS
                     LEK"
     gene            860..>1616
                     /locus_tag="PKI33_14330"
     CDS             860..>1616
                     /locus_tag="PKI33_14330"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNM38243.1"
                     /translation="MSHSEIIATLLQVKPSQVEAVIKLLDDGNTVPFIARYRKEMTGS
                     LDDEQIRIIADELTRLRGLDERRAAILASIEEQGKLTDELRVAIEAAATLTALEDLYA
                     PYKKKRRTRAMVAREKGLEPLADLILKQGSGSLEKLAAKFVNDQVTSIEEALQGARDI
                     VAETISDNANVRSTLREKALKFSTVYAKKIEDAVDEKRVYESYYDFTSRVDRLLPHQI
                     LAITRGEKEGVLKVRVDIPERDWLDAIQSEYEQD"
BASE COUNT          483 a          351 c          332 g          450 t
ORIGIN      
        1 ccgcatgggc ggactgatgg attacaaggc gcgtttctgc tcgccttata taaccatttt
       61 ggaccacaag atattcaatc tgttactata tcaataaatt tccgttttac cagtgaatta
      121 tctaatttga ctcttactca aaggataatt ccataaggat tataaaaatg aaaaaatata
      181 taggattgtt tcttttttgt gtatttatcg tttcttgcgt tcctgtttct gaaaaacttt
      241 ctcagcaaga ttgtaaatat ttactagcaa atattaagga cgaaaatata aatactgatt
      301 ttaggataat tgatgttagt aatgagaaga tttatgagga tttgtataat ttgatagtaa
      361 atgtagacaa tttctcattg tctctactaa cactttcacc taataaggac ttgaaagttt
      421 tctttttgca ggattcaatc tgggtgcagc aagtaaacag tattgaattt cctttagaaa
      481 ttagtcagat tggggttaaa tctcctgacg atccaggtgg aaaaacatat tcttttatgt
      541 ataacccagc caatttagaa ggtacagaaa aaatttgtgt aacaatgata gcgattaaag
      601 acccgcaagg tttatctgag ccgatcggtt catattttga attttcattg gaaaagtagg
      661 atgattaaga gtgtctgtag ctgtttttga ttaagtttat taggatgttt ttctgaaatc
      721 ggaaaattag acgtagaaat taaagaatgt gtagggagct tttcagctat ctctgtaaac
      781 tctataatcg aaaatattac atattcttaa ttatagagtt taccatcctc ttcccatccc
      841 catgataaaa tcgcgcccta tgtctcactc agaaatcatc gcaactcttt tacaagtcaa
      901 gccttcacag gtggaagccg ttatcaaatt attggatgat ggcaacaccg tcccatttat
      961 tgcccgttac cgcaaagaaa tgaccggttc gctcgacgat gagcagatcc gcatcatcgc
     1021 cgatgagctg actcgcttgc gcgggttgga tgagcgccgc gccgccatcc tcgcctcgat
     1081 tgaagagcaa ggcaagctga ccgacgaact ccgcgtcgcc atcgaagccg ccgccacact
     1141 gaccgcgctc gaagacctgt atgcgccgta taagaagaaa cgccgcacac gtgccatggt
     1201 cgcccgcgaa aagggactcg aacccttagc agaccttatc ctcaagcaag gctccggctc
     1261 actcgaaaaa ctcgccgcca aattcgtaaa cgaccaagtg accagcatcg aagaagccct
     1321 gcaaggcgcg cgtgatatcg tcgccgaaac cattagcgac aatgctaacg tcaggtcaac
     1381 tttacgcgag aaggcgctca agttctcaac cgtctatgca aaaaagatcg aagatgccgt
     1441 agatgaaaag cgcgtgtatg aaagctatta cgatttcacc tcgcgcgttg accgtttgct
     1501 tccccatcaa atccttgcta tcacccgcgg cgaaaaagaa ggcgtgttaa aagtccgcgt
     1561 ggatattccc gaacgcgatt ggctcgacgc cattcaatcc gaatacgagc aggata
//
LOCUS       DAOREF010000794         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_69112_length_1613_cov_5.209243, whole genome shotgun sequence.
ACCESSION   DAOREF010000794 DAOREF010000000
VERSION     DAOREF010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69112_length_1613_cov_5.209243"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>1613
                     /gene="pabB"
                     /locus_tag="PKI33_14335"
     CDS             77..>1613
                     /gene="pabB"
                     /locus_tag="PKI33_14335"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNM38244.1"
                     /translation="MIGDLILRKDNAWLKFTGPRRVIVTEKLDEVRNSLEEVERLVNE
                     NGWTAAGFVSYEASPAFDHALRVISSKDGFPLLWFGLYETSETIDSLEDYGTLLPLEW
                     RPDIDREAYNNAIQSIKNQIAQGKTYQVNYTMRLSTDIKAYQQSEIDLQALQLFRHLT
                     HSQNKYAAFLDIGNWAICSASHELFFDLDNDTITGRPMKGTIKRGRNTEEDTNAQNWL
                     RASSKNRAENVMIVDMIRNDIGRIADLGSVHVPELFTIEKYPTLFQMTSTVKGKTKAS
                     VAEIFVNLFPCASITGAPKVSTMNIIADLETSSRNIYCGSIGYIAPGRKARFNVAIRT
                     ALVDKKNRKAEYGIGGGIVWDSTSADEYSEALLKAQVLTSPPQKEFSLFETVLWTPEE
                     GYFLIDRHIARIIDSANYFGFKTENLKENFLKALNAISPKGNSSKRIKISLNSEGFIT
                     TEIKDFQPSAKVFQVCLAKEPVNSMDRFLFHKTTNREVYEKAAITGFDDVLLYNEKDE
                     LTEF"
BASE COUNT          526 a          328 c          335 g          424 t
ORIGIN      
        1 aaaataatcg ccatcattaa aaaggcggga aaccatcgcg gtacaatatc aagaatcttt
       61 ttcatgagag gtatctttga tcggtgattt aattttacgg aaagacaatg cctggctaaa
      121 gtttactggt ccacgccgcg tgattgtaac cgagaagcta gacgaagttc gcaatagctt
      181 ggaagaagta gaaagactag tcaacgaaaa tggctggacc gcagcagggt ttgtaagtta
      241 tgaagcttcc cctgcctttg accatgcatt aagggtgatt tcttcaaaag atgggtttcc
      301 gcttttgtgg tttgggctat atgaaacctc tgagaccata gactcattag aagattatgg
      361 cactctttta cctttggaat ggcgtcctga tattgatcgc gaagcttata acaacgcgat
      421 tcagtctata aaaaatcaaa tcgcccaagg caagacgtat caagttaatt acactatgcg
      481 tttgtcaaca gatattaaag cctaccaaca aagtgaaatt gacttacaag cgctacagct
      541 attcaggcac cttacccatt ctcaaaataa atatgctgcg tttttagata tcggtaattg
      601 ggcgatctgt tctgcctctc atgaactgtt cttcgattta gataacgaca ccatcacagg
      661 tcgccccatg aaaggcacaa tcaagcgcgg tcggaataca gaagaggata caaatgccca
      721 aaattggcta cgcgcatctt ccaaaaacag ggctgaaaat gtgatgatcg tggatatgat
      781 tcgcaacgac atcgggcgaa ttgcggattt aggaagtgtc catgtaccag aattgtttac
      841 tatcgaaaaa tatcccaccc tctttcagat gacatccact gtgaaaggaa aaacaaaggc
      901 atctgtagca gaaatctttg taaacctatt tccgtgcgcg tctattacgg gtgcgccaaa
      961 agtcagcacg atgaacatca ttgcggatct ggaaaccagt tcacgaaaca tatattgcgg
     1021 aagcattggg tacatagctc cgggtcgaaa agctcgattt aatgtagcaa tccgcacagc
     1081 tttggtagat aaaaaaaata gaaaagcaga atatggaatt ggcggaggta ttgtctggga
     1141 ctcaacgtct gcagatgaat atagtgaagc tctgcttaag gctcaggttt taacttcccc
     1201 accacaaaaa gaattctctc tatttgaaac cgtgctttgg acgcctgagg aaggttattt
     1261 tctaatcgat cgccacattg cacgcatcat agattcggca aactactttg gcttcaaaac
     1321 agaaaacttg aaagaaaatt tcctcaaagc cttgaatgct ataagcccaa aaggaaattc
     1381 atccaaacgc ataaaaatat cgctgaattc tgaaggattt attacaacag aaatcaaaga
     1441 ctttcagcca agtgcgaaag tttttcaagt ttgtcttgca aaagaacctg tcaattctat
     1501 ggatcgcttc ttgtttcata aaaccacaaa ccgagaagtg tacgaaaaag ccgctataac
     1561 aggtttcgat gatgtgcttt tatataacga aaaagacgaa ttaaccgagt tca
//
LOCUS       DAOREF010000795         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_69275_length_1611_cov_3.372108, whole genome shotgun sequence.
ACCESSION   DAOREF010000795 DAOREF010000000
VERSION     DAOREF010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69275_length_1611_cov_3.372108"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKI33_14340"
     CDS             complement(<1..502)
                     /locus_tag="PKI33_14340"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM38245.1"
                     /translation="MKKNPFFQFLLILSFSAAACNIGSSTPQEDTALATSAAQTIEAL
                     LTTAPQATPTPPIGGLPPDYTPPATTPAASCEQNYLIDSWLRDNKIYDKAETEKALEP
                     SKGFVMTWKVQNTGPCVWDDTYRMVYDSGERLSTQDSVPVMPNGYTVKNGETLTINIQ
                     MVAPKEV"
     gene            complement(584..>1611)
                     /locus_tag="PKI33_14345"
     CDS             complement(584..>1611)
                     /locus_tag="PKI33_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38246.1"
                     /translation="QVFFVPKEHTLEAILQFRKELATYPSEAPEYIFDFQSGSGYATP
                     FGMLAIGFAIRQFAAARPGSKVKAKNIDKSNYAAHMGYYQCCGFEVGNPPGKAKGSHT
                     YLPVTILNVEELHEQARKAGSEVGDVIEERSQQLARILTQEELGATNDVIAYSLREMI
                     RNVAEHSQSQELAYCAQYHPLKKQAELAILDTGVGIRATLSQNPHLSIHDDREALNLA
                     VMPGISSKMYKGIKKREYDVWQNSGYGLYVVSRLCGHEGKFLICSGDKAMSLKPDARE
                     YHEAHFPGTAVRLNLSSKNISNMSKTLKAISLEGQEQERLMKAVENDHAPVASRMLAS
                     EFSKPKK"
BASE COUNT          320 a          424 c          451 g          416 t
ORIGIN      
        1 cgacttcttt gggcgctacc atttgaatat tgatggtgag cgtctcgccg tttttgaccg
       61 tgtagccatt cggcatcacg ggcacactgt cttgcgtgct caggcgttcg cccgaatcat
      121 agaccatgcg ataggtatca tcccaaacac agggtccggt gttttgcact ttccaggtca
      181 tcacaaagcc cttgctgggt tccaaggctt tctcggtctc ggctttatca tatattttat
      241 tgtcgcgcag ccaggagtcg atcaggtaat tctgttcaca actggcggcg ggggtggtgg
      301 cgggcggggt atagtctggg ggtaagccgc caatgggtgg tgtgggggtg gcttgcggag
      361 ccgtagtgag cagggcttca atggtttgtg cggcagaggt tgccagagcg gtgtcttctt
      421 gtggggtaga agagccaatg ttacaagccg ccgcactaaa gcttagtatc aataaaaact
      481 gaaagaaggg attctttttc acgaagaggg tcctttgtag aggttgaatg cctgcattat
      541 agcgcggagc gctttttctc aacctccctc ctatgtacca cgctcacttc ttgggcttgg
      601 agaattcact tgccagcatg cgcgaagcca ccggggcatg gtcgttctca accgctttca
      661 tcaggcgttc ctgttcctgc ccttccaaac tgatcgcctt gagcgtcttg ctcatgttgc
      721 tgatattctt ggaggataaa ttcaaacgca ccgccgtgcc ggggaaatgc gcctcgtgat
      781 actccctcgc gtctggcttg agcgacatgg ctttatctcc gctgcaaatt aagaacttgc
      841 cttcgtgccc gcacaagcgg ctgaccacat acaagccata gcccgaattc tgccagacgt
      901 catactcgcg tttcttgatg cctttataca tcttgctgga gatgcccggc atgaccgcca
      961 agttgagcgc ctcgcggtca tcatgaatag acaggtgcgg attctgcgaa agcgtagcgc
     1021 ggattcccac gccggtatcg aggatcgcca actcggcttg tttcttgagc ggatgatatt
     1081 gggcgcaata cgccagctct tgcgattgac tatgctctgc cacgttgcgg atcatctctc
     1141 tcagcgagta ggcgatcaca tcgttggtgg cacccagctc ctcctgagtc aagatgcggg
     1201 cgagctgctg cgagcgttcc tcgatcacat cgcccacttc gctgccagcc ttgcgggctt
     1261 gttcgtgcag ttcctccaca ttgaggatcg tgaccggcaa atacgtatgg ctgcctttcg
     1321 ccttgccggg cgggttgccg acttcgaacc cgcagcattg atagtagccc atatgcgccg
     1381 cgtaattgct cttatcgata ttcttggctt tgaccttgct gccggggcgg gctgccgcaa
     1441 attgacggat ggcaaagccg atcgccagca tgccaaaggg tgtggcatag ccgctgccag
     1501 attgaaagtc aaagatatat tcaggcgctt cactgggata cgttgccaac tctttgcgga
     1561 attgcagaat ggcttcgagg gtatgttctt tgggaacgaa aaaaacttgg g
//
LOCUS       DAOREF010000796         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_69336_length_1610_cov_5.870740, whole genome shotgun sequence.
ACCESSION   DAOREF010000796 DAOREF010000000
VERSION     DAOREF010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69336_length_1610_cov_5.870740"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKI33_14350"
     CDS             <1..627
                     /locus_tag="PKI33_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNM38247.1"
                     /translation="VNGGTTVAGTMLAANMSGIKVFATGGIGGVHKESAFDISTDLKA
                     LAETPMVVVCAGAKAILDLPATMEYLETMGVPVIGYKTDEFPAFYSRESGLNVSARLD
                     SPEEIAEFAKAHWSLGLRSAILVTNPVPEADAISKSEMEPMIAQASAEAVEKGIHGQA
                     LTPFLLGRISELTKGKSLKTNLALLLNNARLAGEIANQMYTRKKEWAI"
     gene            665..1054
                     /locus_tag="PKI33_14355"
     CDS             665..1054
                     /locus_tag="PKI33_14355"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNM38248.1"
                     /translation="MKNEFVLSETFNANAAAIYNAWLSTQGHTLMTGSPAKVNGHAKG
                     DFTAWDGYIWGTFLDLEPNKRIVQLWRTGEFPDDAEDSRVEILLEETNGKTKLTLRHT
                     HMPEDQIESYKTGWLDFYFKPMKEYFG"
     gene            1205..>1610
                     /locus_tag="PKI33_14360"
     CDS             1205..>1610
                     /locus_tag="PKI33_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HNM38249.1"
                     /translation="MSITLEQKRLVQETWEKVVPIAETAAELFYGRLFEIDPELKPLF
                     KNADMKEQKMKLLQMLGMAVKGLDNLDQLVPAVENLGRRHVGYGVKDSHYDTVGDALL
                     WTLEKGLGEDFTPEVMEAWTETYVTLATVMKNA"
BASE COUNT          465 a          363 c          400 g          382 t
ORIGIN      
        1 gtgaacggcg gtaccaccgt cgccggtaca atgctcgccg ccaacatgag cggtatcaaa
       61 gtttttgcta caggcggtat tgggggcgtc cataaagaat ctgctttcga tatctccacc
      121 gatttaaaag cattggctga gacccccatg gtagtagtct gtgctggagc gaaagctatt
      181 ttagacctac ccgccaccat ggaatatctc gaaaccatgg gtgtaccggt catcgggtac
      241 aagaccgacg aattccctgc tttctactcc cgcgaaagcg gactcaacgt cagcgcgcgg
      301 ttggattctc cggaagagat tgccgagttt gccaaagccc actggagcct ggggttacga
      361 agtgccattc tcgtgacgaa tccggttccc gaagcagatg ccatctcaaa gtctgaaatg
      421 gagccgatga tcgctcaggc atctgctgaa gctgtagaga aaggcatcca cgggcaggca
      481 cttactcctt tcttgctggg tcgtattagc gaattgacaa aggggaagtc tcttaagacc
      541 aatctggcat tgttattgaa caatgcgcgt ttagcaggtg aaattgcaaa ccagatgtac
      601 acgcgaaaaa aggaatgggc gatttagata gatagcgtag attcgtttga tcgccggagg
      661 caggatgaaa aatgagtttg ttctttctga aaccttcaat gccaacgccg cagccatata
      721 caacgcctgg ttgtccacac agggacatac cttgatgacc ggaagcccgg caaaggtcaa
      781 cgggcacgcc aagggtgatt tcaccgcatg ggatgggtat atttggggca cctttcttga
      841 tctggaaccc aacaaacgca tcgtgcaact ttggcgcacc ggagaattcc ctgacgatgc
      901 cgaagactct cgtgtagaaa tcttgctgga agaaactaat ggcaaaacaa aactcacgct
      961 acgccataca catatgcctg aagatcaaat agagagctat aaaacaggct ggcttgattt
     1021 ttatttcaag ccaatgaaag aatattttgg ataaaataaa aaagagctgc ataaaatgca
     1081 gctctttttt ataggagtaa tgtcctatct gagttttagt accaaactca catggtcaat
     1141 tccaagcgat gcttataaaa tcgcgctcgt attaatcaat taactttaga ggagtatttt
     1201 agaaatgagc atcacattag aacaaaagag acttgtgcaa gaaacctggg aaaaagtcgt
     1261 accgatcgca gaaactgctg ctgaactatt ctacggtcgc ctttttgaga tcgaccctga
     1321 acttaagcct ttgttcaaga atgcagatat gaaagaacaa aagatgaagt tactgcaaat
     1381 gcttggcatg gctgtaaaag gtttggataa cctcgatcag ttagtgcctg cggtggagaa
     1441 tcttggtcgc cgccatgttg gctatggcgt aaaagactct cactacgata ccgtggggga
     1501 cgccctgctg tggacgctgg aaaaagggct cggcgaagac ttcacccccg aagtcatgga
     1561 agcttggaca gagacctacg tcaccttagc aactgtaatg aagaatgcga
//
LOCUS       DAOREF010000797         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_70223_length_1600_cov_5.581230, whole genome shotgun sequence.
ACCESSION   DAOREF010000797 DAOREF010000000
VERSION     DAOREF010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70223_length_1600_cov_5.581230"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKI33_14365"
     CDS             complement(<1..809)
                     /locus_tag="PKI33_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNM38250.1"
                     /translation="MNKIVNDFSITVGTKNGSGSSTANNTILRSIFKMGIPVSGKNLF
                     PSNIQGLPTWYTIRVNKDGFIARKETSDIVIAMNPDSFAKDLASVTPGGAFFYADDIK
                     QPITRVDIAIYPMPVKTIVKNDPNVPTDFRELVGNMVYVGVLAQVIGMDMEAIRAALT
                     FHFKGKQKPIDMNLNAVKAGAEWAAANLEKKDPFYLEHMNKTEGLIMSDGNTAGAIGS
                     IFGGVQFAGWYPITPASSLAEALNDYLPVLRKREDGKHTYAVVQAEDELAAL"
     gene            complement(884..>1600)
                     /locus_tag="PKI33_14370"
     CDS             complement(884..>1600)
                     /locus_tag="PKI33_14370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNM38251.1"
                     /translation="GFHGRKIRLIGLINFMLRDRISPNISEFLRYLASHPEADDKLPS
                     LNELSRELNISLASLREQLEVARALGFVEVRQKTGTRRLTYSFTPAIRQSLGYALALN
                     DEHFRKFAEMRNHLEAAYWYEAVKQLTDEDKKELNAIITRAWEKLQGTHVQVPHEEHR
                     KLHLTIYKNLDNPFVIGVLEAYWEAYEAVGLNVFTGGYEYLKEVWQYHQRMVESICSG
                     KFEVGYEALVRHTDLLYHRP"
BASE COUNT          441 a          377 c          357 g          425 t
ORIGIN      
        1 agcgctgcaa gttcatcttc tgcctgaaca acagcataag tgtgcttgcc atcttcgcgt
       61 ttacgaagca caggcaagta atcatttaaa gcttctgcta aagaagaagc aggcgtgatg
      121 gggtaccaac cagcaaattg aacaccacca aagatagagc caatcgctcc agctgtattg
      181 ccatctgaca tgatcaagcc ttcagtctta ttcatgtgct caagatagaa gggatctttc
      241 ttttcgaggt ttgcagcagc ccattcagca ccagctttaa ctgcattgag gttcatatca
      301 attggctttt gcttgccctt gaaatggaag gtcaacgcag cacggatggc ttccatatcc
      361 atgccaatga cctgtgcaag cacgcctaca tacaccatgt ttccaaccaa ttcacgaaaa
      421 tcggtaggta cattcgggtc gttcttgacg attgtcttaa ccggcattgg gtaaatggca
      481 atgtccactc gggtgatggg ctgcttgata tcgtctgcgt agaaaaaagc cccacctggc
      541 gtaacagaag cgaggtcctt tgcgaacgaa tcggggttca tcgcaatgac aatgtcactg
      601 gtttccttac gggcgatgaa accatcctta ttcacacgaa tggtgtacca cgtaggcaga
      661 ccttggatat tggaagggaa caggttcttg ccagagacgg ggatgcccat cttaaagatg
      721 gatctcaaaa tggtgttatt ggcagtggaa ctgccggaac cgttcttcgt accaacggtg
      781 atcgaaaaat cgttcactat cttattcatg gaactcctca taaaaattag gtcgatgtac
      841 agagacggtt atagcctcat ctgtgcctat cgacgaaaat gacctaggga cgatgataca
      901 aaaggtcagt atgacgtaca agcgcctcat acccgacctc aaacttgcca ctacaaattg
      961 actcgaccat acgctgatgg tattgccaca cttctttcaa atattcatac ccgcccgtga
     1021 acacatttaa acccactgct tcataggctt cccaatacgc ttctaataca ccgatcacaa
     1081 aagggttatc caagtttttg taaatggtaa ggtgtaactt acggtgttct tcatggggaa
     1141 cttgcacatg cgtgccttga agtttctccc aagcgcgtgt aatgatggcg ttgagttctt
     1201 ttttatcttc atccgtcaat tgcttaaccg cttcatacca ataagcagct tcaagatgat
     1261 tacgcatttc tgcaaatttc cgaaaatgct catcattcag tgccaaggca tatcccaaac
     1321 tctggcggat tgctggagtg aaggaataag tcaatcgccg cgttccggtc ttttgacgaa
     1381 cttcgacaaa gccaagcgcg cgagcaactt ctagttgttc gcgtagtgat gcaaggctaa
     1441 tattcaactc tcggctaagt tcgtttaaag aggggagctt gtcatctgct tcagggtgag
     1501 acgccagata acgaagaaat tcggaaatgt tcggtgagat acggtcacgt agcataaaat
     1561 tgattaatcc gattaatcgt attttacgcc cgtgaaagcc
//
LOCUS       DAOREF010000798         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_70325_length_1599_cov_4.512306, whole genome shotgun sequence.
ACCESSION   DAOREF010000798 DAOREF010000000
VERSION     DAOREF010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70325_length_1599_cov_4.512306"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKI33_14375"
     CDS             <1..360
                     /locus_tag="PKI33_14375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM38252.1"
                     /translation="YYYSAAEILVMPSLYESFGMVALEAMACGTPVIASEVGGLGYLV
                     QDGVTGYTVPDSAPEALCEKLSILLGNSDLRNEMGMKAAEYATDYAWEKIAAGIINVY
                     EEVSTVHHIVDVKPPRT"
     gene            complement(387..1136)
                     /locus_tag="PKI33_14380"
     CDS             complement(387..1136)
                     /locus_tag="PKI33_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNM38253.1"
                     /translation="MSEDSLRTLSRSKIAMLIDGDNAQAGLLSQMLVEAGRHGQVTLR
                     RIYGDWTTNNMNSWKDTLNFHAFQPIQQFRYTVGKNATDSAMIIDAMDILHSGVVDGF
                     CLVSSDSDYTRLATRIRETGIFVMGIGEKKTPKAFVNACDVFVYTENLVTAKKSTQQQ
                     KPNKGGAKKKDELDPIPLLTQAFEMAVQQDGWAALSNMGNALYQLDPAFDPRTYGHKQ
                     LSKMISKFKDRFEVRLQEIDGSTLFHVKLKE"
     gene            1277..>1599
                     /locus_tag="PKI33_14385"
     CDS             1277..>1599
                     /locus_tag="PKI33_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38254.1"
                     /translation="MKEKFLALVNLKPEELRLAFSLWVLIAVNTFVLELADVVATAGF
                     VGHLGVDRIPWLWIVTTIITIFAAGFYLVFIDRYQRLQLVTWLLTGLAILYLLLEFLF
                     AFNAPT"
BASE COUNT          373 a          322 c          401 g          503 t
ORIGIN      
        1 tactattatt cggcagcaga aatattggtt atgccttcgt tgtatgaatc gtttggaatg
       61 gtggcattgg aggcaatggc ttgcggcaca ccggtgattg cttcagaagt gggcgggctt
      121 ggttatttag ttcaagatgg tgttactggc tacaccgtgc ccgatagtgc cccagaggct
      181 ttgtgcgaaa aactttcgat ccttttggga aattctgact tgcgaaatga aatggggatg
      241 aaagctgctg aatacgcaac cgattatgct tgggaaaaaa tcgcagccgg gattatcaat
      301 gtatatgaag aagtttctac tgtacatcac atagtggatg taaaaccgcc tcggacttaa
      361 ctcgaggcgg tttgctgctt ccaaacttat tctttcaatt tgacatggaa caaggttgag
      421 ccatctattt cttgtaagcg aacctcaaac cggtctttga atttactgat catttttgag
      481 agttgtttgt gtccgtatgt acgcgggtca aatgctgggt ctaattggta taaggcattt
      541 cccatatttg aaagcgcagc ccaaccatct tgttgaaccg ccatttcaaa ggcttgagtg
      601 aggaggggaa tcgggtctag ctcgtctttt ttcttcgctc cgcctttatt gggcttttgc
      661 tgttgagttg attttttggc ggttacaagg ttttctgtgt atacaaaaac atcacaggca
      721 ttgacgaagg ctttcggggt tttcttttct ccgatgccca tcacaaaaat gccagtctcg
      781 cggatgcgcg tagccagcct agtatagtcg ctgtcactgg agactaggca gaaaccatct
      841 actacgccag agtgcaaaat atccatggca tcaatgatca tggcgctgtc tgtggcgttc
      901 ttgccgactg tatatcgaaa ctgctgaatg ggttgaaagg cgtggaagtt tagtgtgtct
      961 ttccacgaat tcatgttatt ggttgtccaa tcgccataaa ttcgccgaag ggtcacctgt
     1021 ccatgccgac cggcttcaac cagcatctga gagagtaacc cagcttgggc attgtccccg
     1081 tcaatgagca tggcaatctt gctgcgagag agagttcgta atgagtcttc tgacataatg
     1141 aaattatacc tgtttgcgct ccgtttccgc gcatggggaa gggttgttct agcacctgta
     1201 ttgggattac attactgaaa acttggctta agggtcctat gataaaattt gaaatcttta
     1261 tagcacagag gctttcatga aggagaaatt tctagcgctc gttaacttga aacctgagga
     1321 attgaggctg gcattttctc tttgggtttt aattgccgta aacacctttg ttttggaatt
     1381 ggcagatgtt gtagctacag cggggtttgt tgggcatttg ggcgttgacc gaattccatg
     1441 gctttggatc gtgactacta tcattacgat ttttgctgcg gggttttatc ttgtttttat
     1501 tgaccgatac cagagattgc agttggtcac atggctgttg accgggcttg ccattctata
     1561 ccttttgctt gaattcttgt ttgcattcaa tgcgccgac
//
LOCUS       DAOREF010000799         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_70832_length_1594_cov_2.158545, whole genome shotgun sequence.
ACCESSION   DAOREF010000799 DAOREF010000000
VERSION     DAOREF010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70832_length_1594_cov_2.158545"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1594
                     /locus_tag="PKI33_14390"
     CDS             <1..>1594
                     /locus_tag="PKI33_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM38255.1"
                     /translation="QIHPYQQKLGVKAVAAFPLTVADDVLGVLYIYLYEDRHLSQFEE
                     LMLHNFVNQAAMAIHHARNIAGIRQNLIQKENELSRLRRAGLLISSRQKLEETLESIL
                     NLALELTNAQYGIFRLLDKSGEFLVTRAVSSLQLERPLVEKLPISGKSMMAHVARERQ
                     PLLIRDLREKPWSDVYYPLDSVLEMRSELCVPIINASGRLEGVLNLESPQVGGFSEDD
                     SHMLQSLATYAVTAIQEVRLLDALQEAAQLLIAQPSQTVLSQLCVMANDLLNASTSAI
                     WLQNENGEITLASANGEAVPEKAPTVADDNALVMPLLSGEKLAALGVFGAFYAGTSGV
                     RSGSSEWDRKVLACLANYAVLAVQNESRQQALRASQQQHLIAETFAAVGDISANLLHN
                     MNNKVGVIPVRVQGIQDKYAKLLGEEAYLAKSLSEIERSATAAMQIVQENLSHLRPIR
                     LERIQVAPRVNDAIRAVSAPADVHIEVDGLENLPTVNASGQSLVFVFRNLIENAIDAM
                     NGKGEIKIRGEQNGSWVDVSVSD"
BASE COUNT          427 a          396 c          389 g          382 t
ORIGIN      
        1 gcagattcac ccctatcaac aaaaacttgg ggtcaaagcc gttgccgcct tcccgttaac
       61 agttgcagat gatgtcttgg gtgtgctcta tatctatcta tacgaagacc gccacctctc
      121 gcagttcgaa gagttgatgc tgcataattt cgtgaatcaa gccgcaatgg caattcacca
      181 cgcccgcaac attgcaggga ttcgtcaaaa cctcattcaa aaagaaaatg aactctctcg
      241 gttgcgccga gcagggttat tgatctcttc ccgccaaaag cttgaagaaa cacttgagtc
      301 cattctcaac ctcgcccttg agttgaccaa tgcccagtat ggcatcttcc gcctgctgga
      361 taaatctggt gaatttctcg tcacacgtgc ggtcagttct ttgcaattgg aacgtccgct
      421 tgtggagaag ttgccgatca gcgggaaaag catgatggca catgttgcca gagaaaggca
      481 gccattacta attcgtgacc tgcgcgaaaa accctggtca gatgtgtatt acccgctcga
      541 ttctgtattg gaaatgcgct ctgaattgtg tgtgccaatc atcaatgcca gtggacgtct
      601 cgaaggcgta ttgaacttgg aaagcccaca agtaggtggc tttagcgaag acgacagtca
      661 tatgttgcaa tcgcttgcta catacgccgt gactgcaatt caagaagtcc gcttgctgga
      721 tgcgttacaa gaagccgcac aactccttat tgcacagccc agccaaaccg tactaagtca
      781 actttgcgta atggcaaatg atttactgaa tgcctccaca agtgccattt ggctgcaaaa
      841 tgaaaatgga gagataactc tcgcatccgc taacggcgag gctgtgccag aaaaagcacc
      901 aactgtagca gatgacaatg cattggttat gcccttacta agcggagaaa agctcgcagc
      961 gttgggagtg tttggggcgt tctatgcagg cacgagcgga gtccgaagcg gttcctctga
     1021 atgggaccgg aaagtgttgg catgtctcgc caactatgcc gtgcttgccg tccaaaacga
     1081 atccaggcaa caagccttac gtgcttcgca gcaacaacat ctcatcgcag aaacttttgc
     1141 ggctgtcggt gatatttctg cgaatctctt gcacaacatg aataacaaag tgggtgtaat
     1201 tcctgtacgc gtgcaaggca ttcaagataa gtatgccaaa ctattgggtg aagaagccta
     1261 tctcgccaag agtttaagcg agatcgaacg aagcgccacc gccgcgatgc agattgttca
     1321 ggaaaatctt tcgcacttac gccccattcg tttggagcgc attcaagtag ccccacgcgt
     1381 caatgatgcc attcgcgcgg tttctgctcc agcggatgta cacattgaag tggatgggct
     1441 tgaaaatcta cccactgtta atgccagcgg acaaagcctc gtgttcgtct ttcgcaacct
     1501 gatcgaaaac gccattgatg ccatgaacgg caaaggcgaa ataaaaattc gcggcgaaca
     1561 aaatggcagc tgggtggatg tttctgtaag tgac
//
LOCUS       DAOREF010000800         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_70846_length_1594_cov_1.618583, whole genome shotgun sequence.
ACCESSION   DAOREF010000800 DAOREF010000000
VERSION     DAOREF010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70846_length_1594_cov_1.618583"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1594
                     /locus_tag="PKI33_14395"
     CDS             <1..>1594
                     /locus_tag="PKI33_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HNM38256.1"
                     /translation="CSSSLVAAHLACQSLQNGESDLGVVGGVNLILTPELTITFSQAR
                     MLSPDGRCKTFDASADGYVRGEGCGVIILKRMSDAIRDGDNILALIRGSAVNQDGRSN
                     GLTAPNGLAQQDVIRQALANAQVAPHQIGYVEAHGTGTPLGDPIEMASLHAVLDDGQS
                     NNRVLVGSVKTNIGHLESAAGIAGLIKSVLAMQHEAIPPHLHLKEVNPYLSLENSRLE
                     IGTYLRPWKRREQPRFAGVSSFGFGGTNAHIILSDAPMLAPSTPSENIERPRHIFALS
                     GKTESSLLELAEQTSAQLESTQLSISDYGFTVNTTRSHFEHRLAIQASTPAELKAGLD
                     AFISNTNIPALSTGHAKPGTQPKIAFLFTGQGAQYIGMGRGLYETQPVFRAALDQCAE
                     ILNSILDRPLLDVMYPQDKNDTLLNQTTYTQPALFAFEYALAMLWRSWGFEPQAVLGH
                     SVGEYVAACIAGVFTLEDGLRLIAERARLMGSLPNNGTMAAVFADASRVADALKPYQS
                     QVSIAAVNGPDNTVISGEKFAVQ"
BASE COUNT          356 a          415 c          428 g          395 t
ORIGIN      
        1 ttgttcatcg tctttggtgg cggcgcatct ggcgtgtcag agccttcaga acggtgaatc
       61 agatttgggc gtggtcggag gcgtgaactt aatcctcact cccgaattga ccatcacctt
      121 ctcccaagcg cggatgttat ctcccgatgg acgctgcaaa acattcgatg ccagtgcgga
      181 tggatacgtg cgcggcgaag gctgcggggt gattattttg aagcgcatgt ccgatgcgat
      241 tcgcgatgga gataacatcc ttgcattgat ccgcggctcg gcagtgaatc aggatggacg
      301 cagcaatggc ttgaccgccc cgaacggact cgctcaacaa gatgtcattc ggcaggcgtt
      361 ggcgaatgcg caggtcgcgc cgcatcagat cggttatgtc gaagcgcacg gcacaggcac
      421 gccacttggc gacccgatcg agatggcgtc gttgcacgcg gttcttgatg atgggcaatc
      481 aaataataga gtgttggtcg gttcggtaaa aacgaacatc ggtcatcttg agtctgcggc
      541 gggaattgcg gggctgatca aatcggtgct tgccatgcag cacgaagcca ttccgccgca
      601 ccttcattta aaagaagtca acccatattt gtctttggaa aattcgcgcc ttgaaattgg
      661 aacctacctg cgtccgtgga agcgccgcga gcaaccgcgc tttgcgggcg tgagttcttt
      721 tggctttggc ggcacgaacg cgcacatcat tctttccgat gcgccaatgc ttgcgccatc
      781 aacgccgtca gaaaatatcg aacgtccgcg ccacattttt gctttatctg ggaagacaga
      841 gtcgtcatta cttgaattgg ctgaacaaac cagcgctcaa cttgagtcaa cgcaactgtc
      901 tataagtgat tatggtttta cagtaaacac aactcgttcg cactttgaac atcggcttgc
      961 tattcaagcc agcacacctg ccgaactgaa agctgggttg gatgcattta tttccaacac
     1021 gaatatcccc gccttatcaa cgggtcatgc aaaacccggc acacaaccga agattgcttt
     1081 cctgttcacg gggcagggcg ctcaatatat cggcatgggt cgcggacttt atgaaacgca
     1141 acccgtcttt cgcgccgcgc tggatcaatg cgccgaaatt ctgaattcca ttttagatcg
     1201 tccgttgtta gacgtgatgt atccgcaaga taaaaacgat acgttgctca atcaaaccac
     1261 ctacacacag cccgcgctct ttgcttttga atatgcctta gccatgctgt ggcgttcttg
     1321 gggttttgaa ccgcaagctg ttcttgggca tagcgttggt gaatatgttg ccgcatgtat
     1381 cgcgggtgtc ttcaccttgg aagatggtct gcgtctcatt gccgagcgcg cgcgtttgat
     1441 gggcagcctt ccgaataacg gcacgatggc ggcggtcttc gcggatgctt cgcgtgtggc
     1501 agatgctttg aagccgtatc aatctcaagt ttccatcgcc gctgtgaatg gacccgacaa
     1561 cacggttatc tctggcgaga aattcgccgt gcaa
//
LOCUS       DAOREF010000801         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_70934_length_1592_cov_3.420950, whole genome shotgun sequence.
ACCESSION   DAOREF010000801 DAOREF010000000
VERSION     DAOREF010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70934_length_1592_cov_3.420950"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PKI33_14400"
     CDS             <1..929
                     /locus_tag="PKI33_14400"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNM38257.1"
                     /translation="EGTPATVHAGPFANIAHGQSSIIADKIALKLVGKDGFVVTESGF
                     GADIGMEKFFNIKCRYSGLIPHVVVMVATVRALKMHGGGPKVVAGKPLAPEYTDENLE
                     LLKAGLVNLERHIQNAMKFGVKVIVAINSFATDTEAEVELIRKSAIEFGAMDAIKCTH
                     WADGGKGTKKLAEAVVKAAEQPSNFKFLYDPKLPIKEKIEIIAKEIYRADGVDYSPEA
                     EEQVERYTRLGFNDLPICMAKTHLSFTTDATKKGAPTGFRITVREVRASVGAGFLYPI
                     LGDMRTMPGLPTRPSFYDVDLDMETGKVLGLF"
     gene            1038..>1592
                     /locus_tag="PKI33_14405"
     CDS             1038..>1592
                     /locus_tag="PKI33_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNM38258.1"
                     /translation="MNPASLTLAEQRELIKSKEINASDLAAACYRQIDRLNPMLNAFI
                     TVIPQDDEEVLDNRHMALLQGIPIAVKDLYNTKGIRTTGGSKFFADYVPSEDAFVVDK
                     IKKAGAHIIGKTNTHEIALGVTNNNPHFGACRNPWDIAHTPGGSSGGSAVAVATGMAL
                     AAIGTDTGGSIRIPASLCGVVGLKP"
BASE COUNT          413 a          407 c          383 g          389 t
ORIGIN      
        1 tcgaagggac tcccgccacg gttcacgccg gaccattcgc caatatcgcc cacggacagt
       61 cttctatcat cgctgacaaa attgcactca agttagtcgg caaagatggc ttcgtggtca
      121 ctgaatctgg tttcggtgcc gatattggta tggaaaagtt cttcaatatc aaatgccgct
      181 attctggctt gatcccgcat gtggtggtga tggttgcgac cgtccgcgca ttgaagatgc
      241 atggcggtgg tccgaaagtg gtggcgggta aaccgctcgc ccctgaatac accgacgaaa
      301 atctggaatt gctcaaggcg ggtctcgtta accttgaacg ccatattcag aatgcaatga
      361 aattcggtgt caaagttatt gttgctatca actcctttgc cacagataca gaggctgagg
      421 ttgaactcat ccgcaaatct gccattgaat tcggcgccat ggatgccatc aaatgtacgc
      481 actgggctga tggcggtaaa ggtaccaaga aattggctga ggcggtggtc aaagctgcag
      541 aacagccgag caacttcaag ttcctgtatg atccgaagct tcccatcaag gaaaaaatcg
      601 agatcatcgc caaggaaatc tatcgcgctg atggtgtgga ttactctcct gaagccgaag
      661 aacaggtcga acgctatacc cgcctcgggt tcaacgacct tccgatctgc atggcgaaga
      721 ctcatctttc ctttactacc gacgccacca agaaaggtgc gcctacgggc ttccgtatca
      781 ccgtccgcga agtgcgtgcc agcgttggtg caggcttctt gtaccccatc ctcggcgaca
      841 tgcgcaccat gcccggtttg cccactcgcc catctttcta cgatgtggac ttggatatgg
      901 aaacaggcaa ggtcctcggg ttgttctaga acaatctcta cctacaaaaa tgcaggtcac
      961 gcgttaggcg tgacctgttt tttcagtacc gataggctat attgaaaata agtataaatt
     1021 gaatataatt cacccttatg aatcccgctt ccctgacttt agctgaacaa agagaactga
     1081 tcaaaagtaa ggaaatcaac gcttctgacc ttgccgcggc ctgttaccgt caaattgacc
     1141 gtctcaaccc gatgcttaat gccttcatca ctgtcattcc gcaagatgat gaagaggtgc
     1201 ttgataaccg acacatggct cttttacaag gcattccgat tgcagttaaa gatttataca
     1261 ataccaaagg catccgcaca acgggtggct ctaaattctt cgcagactat gtgcctagtg
     1321 aagatgcatt cgttgtggat aagatcaaaa aagcaggagc gcatattatt ggcaaaacca
     1381 atacccatga gatcgctctt ggtgtaacca ataacaatcc gcattttggg gcatgccgca
     1441 acccttggga tattgcccac accccgggcg gctcatcagg cggaagcgca gttgcagtgg
     1501 caacaggcat ggcacttgct gctataggca ctgatacagg cggttccatt cgcattccag
     1561 cttcattatg tggtgtagta ggactcaaac cc
//
LOCUS       DAOREF010000802         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_71057_length_1591_cov_2.148438, whole genome shotgun sequence.
ACCESSION   DAOREF010000802 DAOREF010000000
VERSION     DAOREF010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71057_length_1591_cov_2.148438"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKI33_14410"
     CDS             <1..312
                     /locus_tag="PKI33_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNM38259.1"
                     /translation="EIDEGRRKIVGVNAYMDEKPAAIPILRMDPNGYTRQMSRLNEVK
                     KTRDSGRVGQTLDALHIACEGTENTMPYIIDCVRAYCTLGEIVGVMTKVFGKYEEPTM
                     I"
     gene            complement(372..>1591)
                     /locus_tag="PKI33_14415"
     CDS             complement(372..>1591)
                     /locus_tag="PKI33_14415"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM38260.1"
                     /translation="VKSKLGRRRPFLLWFAFPFGLSFVMLWSAPNWDSQIALLIYVTL
                     AFMISDTLTTLVAVPYYSLTPELTQNYDERTSLSSFRSVFQLLSAMVVVVSAPMIVDA
                     VILGGGSQQQGFLTAGAVFGAIGSLPLFLIGLFVRERFSEDSQEQEELSFRETLKLAW
                     QNVPFRYAVGIYMFNWSAVDMVSITFPFFLLYWVAQGDLLAKINILGVDLALESAFFG
                     VLMLVCVLFVPFWLWLSKRQNKIRAYILGMAAWVIVQMLIFTIQPGETSYMLFLAGLA
                     GIGVSAAYILPDSILPDVIEWDELRTGRRQEGIYYGIRTLIRKLTGALIIFITLQILG
                     WSGYQAPPENVTVYQQPETALFMIRLMDSFIGATILTGTIILAWSYPLTREKYQRIQK
                     LLAVRKSKKVETA"
BASE COUNT          495 a          381 c          377 g          338 t
ORIGIN      
        1 gagatcgatg aagggcgtag aaagatcgtc ggtgtgaatg catatatgga tgaaaagcct
       61 gcagcgattc ccattttacg catggacccg aatggataca cccgtcagat gtcacgcttg
      121 aacgaagtga agaagacccg tgatagcgga cgcgttgggc aaacgctcga tgcactgcac
      181 atcgcctgcg aaggcaccga gaacacgatg ccttatatca ttgattgtgt gcgtgcgtat
      241 tgcacattgg gagagatcgt tggggtgatg acgaaggtct ttggaaagta cgaagagcca
      301 acgatgatat aacaccaccg ctcttgtcgg ggcttagccc cgacaagagc atacaaaaaa
      361 ggtggaaacg gttatgccgt ttccaccttt ttgcttttgc gaactgccaa taatttttga
      421 atacgttgat atttttcacg tgtcagcgga tacgaccatg ccaaaatgat cgtgccagtc
      481 agaatcgttg cgccaatgaa cgagtccatg aggcggatca tgaataaagc agtttcgggt
      541 tgttgataga ctgttacatt ttccggcggc gcctgataac cagaccagcc caaaatttgc
      601 aacgtgatga agataatcaa tgcgccggtc aatttgcgga tgagagtgcg aatgccgtaa
      661 taaatacctt cttgtctgcg ccccgtgcgg agttcgtccc attcgatcac atccggcaag
      721 atggagtctg gcaggatata agcggcagaa acgccaatgc ccgccaaccc ggcaaggaac
      781 aacatataac tggtttcacc cggttgaatg gtaaagatca acatctgcac aatcacccat
      841 gccgccatgc caaggatata agctcgaatt ttgttttggc gctttgaaag ccatagccag
      901 aatggcacga acagaacaca gaccaacatc aacacaccaa agaatgcaga ctcaagagca
      961 aggtctacgc ctaaaatatt gatcttcgcc agtaaatctc cctgtgccac ccaatacaac
     1021 agaaagaatg gaaaggtgat cgagaccata tccacagcag accaattgaa catataaatc
     1081 ccaaccgcat accgaaaagg aacattctgc caagccagtt tgagagtctc gcgaaatgaa
     1141 agttcctctt gttcctggct atcttcagaa aaacgttcac gtacaaagag accgatcaaa
     1201 aacaaaggca atgaaccgat cgcaccaaac acagctcccg cagtaagaaa accttgttgt
     1261 tgcgagccgc cgcctagaat aaccgcatcc acgatcatag gggctgaaac caccactacc
     1321 atggcagaaa gcaattgaaa gaccgagcga aaacttgaaa gcgaggtgcg ctcgtcgtaa
     1381 ttttgagtta gctcaggagt gagtgaataa tacggaaccg ccaccaaggt tgtgagcgtg
     1441 tcagagatca tgaatgccaa ggttacatag atcaacaaag cgatctggct atcccaattt
     1501 ggcgcagacc atagcatcac aaagcttaaa ccaaatggga aggcaaacca taataagaaa
     1561 ggtctgcgtc tgccaagttt cgatttcact c
//
LOCUS       DAOREF010000803         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_71187_length_1589_cov_3.406780, whole genome shotgun sequence.
ACCESSION   DAOREF010000803 DAOREF010000000
VERSION     DAOREF010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71187_length_1589_cov_3.406780"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKI33_14420"
     CDS             complement(<1..214)
                     /locus_tag="PKI33_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38261.1"
                     /translation="MQYKTRQFIFHRTYEMASLVEACVVDSSSFIEIDWEKPEVLRCL
                     VEFSKVSLLHYYIYSMISANERQNLFD"
     gene            complement(544..>1589)
                     /locus_tag="PKI33_14425"
     CDS             complement(544..>1589)
                     /locus_tag="PKI33_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNM38262.1"
                     /translation="RMPNRKIEPAHLLVVDDNKVNRMALSRSLELQGHMVETAENGKE
                     GLEKFQTGTFDLMLLDIEMPVMNGFEVLEACLNDVDLRQIPIIMTSAMEELDAVVKCV
                     ELGAEDYLTKPVNPILLRARVNASLEKKRLRDEQRKLFRTFATKEVAEELLRTGFSLG
                     GQIVTASVLVADIRSFTSIAESQDPSDSIELLNQYFSMMFDAVTHNGGIINQIVGDGL
                     LAVFGAPIFYEDHRERAVKAALQMLEQLKLFNEQQLAANKTAIRIGIGIASGRMVAGY
                     TGTHNRAIYTCIGDTVNLADRIEEYTKEALRPLLIDAYTRDGLSDQFVVEDLGKIVFK
                     GKSQAVNVFAVMK"
BASE COUNT          412 a          441 c          312 g          424 t
ORIGIN      
        1 tgtcaaaaag attctgccgc tcattagctg aaatcatcga gtaaatatag taatgaagaa
       61 gacttacctt ggaaaattcc actaaacatc ttaagacttc aggtttttcc caatcaattt
      121 ctataaatga tgacgagtct acaacgcagg cttcaaccaa tgaagccatt tcgtaagttc
      181 tgtggaagat aaattgacga gttttatatt gcatgtgttt ttcttattgc gccagccgcc
      241 taatggtttg ctgcccgtcc ctgataggag atacctgccc cgttgcgtag acttgcctca
      301 tccgtccagc gagaaataaa gcttgatgta ttcagttatt tccaatttta tcagttgttc
      361 tataaatgct cgcttagctg caaacagcgg gacgtaaacg atgaaaacca ggttgtataa
      421 catccctgcc caaaaactca accaataaga gcctttccag tccttaatct cgatgtaact
      481 gttttcttgc gagtttataa agcttgcata cataccaagc agtccaaaca acgcaccttt
      541 aactcatttc attaccgcaa acacattcac agcctggctc ttgcccttga aaacgatctt
      601 cccaagatct tccaccacga actggtctga caagccatca cgggtatatg catcgatcaa
      661 gagcggacgg agcgcttcct tggtgtattc ctcgatccgg tccgctagat tcaccgtgtc
      721 accaatgcag gtgtaaatgg cgcggttatg cgtccctgta taacctgcta ccatccgccc
      781 tgaagcgatc ccgatcccga ttcgaatcgc ggtcttgttc gctgccagtt gttgctcgtt
      841 gaacagtttc aactgctcca gcatttgcaa tgcggctttc actgcccgtt cgcgatggtc
      901 ttcataaaag atcggcgcac cgaacactgc caacaagcca tcccctacga tctgattgat
      961 aatgccgccg ttatgggtca cggcatcgaa catcatactg aaatattggt taagcaattc
     1021 aatcgagtca gaagggtcct gactctctgc aatggaagtg aacgaacgga tatccgccac
     1081 caacacactc gccgttacga tctgcccgcc caacgaaaag cccgttcgca gcaattcctc
     1141 tgctacttct ttcgtcgcaa aggtacggaa taacttccgc tgttcgtctc gcaagcgttt
     1201 cttttcaagg ctggcgttga cgcgcgcccg cagcagaatc gggttcaccg gcttggtcaa
     1261 ataatcttca gcacccaact ccacgcattt cactaccgca tccaattctt ccatggccga
     1321 agtcataatg atgggaattt gccgtaagtc aacatcgttc aagcaagctt ccagcacctc
     1381 aaatccgttc atcactggca tctcaatatc cagcaacatc agatcaaaag ttccggtctg
     1441 aaacttctcc aagccctctt tgccgttctc ggcggtctct accatatgcc cctgcaactc
     1501 aagactgcgc gaaagcgcca tccggttaac cttgttatcg tccaccacca acagatgagc
     1561 aggttctatt tttcgatttg gcatccgat
//
LOCUS       DAOREF010000804         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_71266_length_1588_cov_3.875408, whole genome shotgun sequence.
ACCESSION   DAOREF010000804 DAOREF010000000
VERSION     DAOREF010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71266_length_1588_cov_3.875408"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKI33_14430"
     CDS             <1..383
                     /locus_tag="PKI33_14430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38263.1"
                     /translation="MNIIRNFVLVMFLAGGLAGLAGTAQVLGVDHWVGQGFSAGYGFD
                     AIALALLGRSHPAGVVLAALLFGFLRSGATDMQSMAGIPIDIISIIQGMVIVFIAAPD
                     IIRWLYRIRATKKDDAVLTRGWGK"
     gene            395..>1588
                     /locus_tag="PKI33_14435"
     CDS             395..>1588
                     /locus_tag="PKI33_14435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38264.1"
                     /translation="MKNTKPEISSNTEKRQRISYGLVLLAVGLFIYFVFGLQTEAGTV
                     AKFGLNLAGSQAIEISDLSVPALPVVYFMAFLAVFAGAFQLARGVRSTNWLVGILAVT
                     IVVSFLMWAAQDKSFNLTGMLSSSFVRATPIALAALCGVISERSAVINIGIEGIMLIS
                     AQLAVLTATVTKSLYVGLFAAIIAGGLIAALHAYLVIRFKVDQTVSSVAINIFGAGTT
                     AFISSRFMQRAGDQLNNSGTFPIISIPGLSKIPVLGPVLFENNLIIYLAIILVFVMHI
                     VLYYTPWGLRTRAVGEHPKAADTLGINVYFTRYINVIAGGMIAGLGGAYFTLGSVGRF
                     DEAMTAGKGFIGLAAMIFGNWNPIGAYTSSLIFGFADSLQVKLQILRIPIPSEFLLMA
                     PYIVTM"
BASE COUNT          323 a          387 c          393 g          485 t
ORIGIN      
        1 gcatgaacat catccgtaac ttcgtgttgg tcatgttcct ggcaggtggt ttggcaggtt
       61 tggctggaac tgctcaagtt ctcggcgtag atcactgggt gggtcagggc ttttcagccg
      121 ggtacgggtt tgatgcgatc gcattggcgt tgctgggacg cagtcatccg gcaggagtcg
      181 tccttgcggc gctgctattt ggcttcctcc gtagtggtgc caccgatatg caatctatgg
      241 ctggcatccc catcgacatt atctcgatca ttcaaggtat ggtgattgtg tttatcgccg
      301 cacctgatat catccgctgg ctttaccgca tccgcgccac gaaaaaggat gatgctgttc
      361 ttacccgtgg ttggggtaaa taaacgaggc ttgcatgaaa aacaccaaac ctgaaattag
      421 ctctaatact gagaaacgcc agcgcatttc atatgggctt gtattgcttg cggtgggtct
      481 gttcatttac tttgtctttg gtttacagac agaagctggc acagttgcca aattcggttt
      541 aaatttagca ggctcacaag ccattgaaat ttcagacctt agtgttccgg cattgcctgt
      601 tgtgtatttc atggcatttc tcgctgtttt tgccggtgcc tttcaattgg cacgtggggt
      661 gcgctctaca aactggttgg ttggaattct tgcagttacc attgtcgttt cctttttgat
      721 gtgggcagcg caagataaat ccttcaacct gaccggtatg ttgagtagtt cattcgtccg
      781 tgctacaccg attgctttag ccgccttatg tggtgtgatc agcgaacgct cagcggttat
      841 caacatcggt atcgaaggca ttatgctcat ctcagcacag cttgctgtgc tcactgctac
      901 ggtgacgaaa agcctgtatg ttgggctctt tgctgccatt attgcaggtg gtttaatcgc
      961 tgccttgcat gcctatttgg tcattcgctt caaggtagac caaactgtca gtagtgttgc
     1021 catcaacatc ttcggtgccg gtacaactgc ttttatcagc tcacgcttca tgcaaagagc
     1081 gggtgatcaa ttaaacaatt ctggcacctt ccccatcatc tccattcctg ggctttctaa
     1141 aatccctgtg ttgggacctg ttctcttcga aaacaatctg atcatttatt tggcgatcat
     1201 tttagttttc gtgatgcaca tcgtgctgta ctacactcct tggggcttgc gcacgcgtgc
     1261 ggtgggtgaa caccccaaag ctgcagatac cttgggtatc aatgtgtact tcacccgcta
     1321 tatcaacgtc attgctggcg gtatgatcgc tgggttgggt ggcgcctact tcaccctcgg
     1381 ttccgtaggt cgttttgacg aagccatgac tgcgggtaaa ggtttcatcg ggctcgccgc
     1441 tatgatcttc ggaaattgga atcccatcgg tgcgtacact tcatcattaa tctttggttt
     1501 cgcagactcg ttacaggtca aactacaaat cctgcgcatt ccgatcccct cagaattctt
     1561 gctcatggcg ccatacattg tgaccatg
//
LOCUS       DAOREF010000805         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_71544_length_1585_cov_3.062092, whole genome shotgun sequence.
ACCESSION   DAOREF010000805 DAOREF010000000
VERSION     DAOREF010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71544_length_1585_cov_3.062092"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKI33_14440"
     CDS             complement(<1..882)
                     /locus_tag="PKI33_14440"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNM38265.1"
                     /translation="MNFGSHRPYRHGTNLWLFTLFLAVSCAEPAPASTPDPTLVPLQV
                     NYQPTPTPFQPQPGTSPDPYLAISTPQDAVPTYTPVPTKYVPPQDVSVPVDVLPAAQP
                     GVTLYNPLTGLPVSDPYFLQHRPLAIKVGNSPDYVRPQSGLTLADVVYEYYIEWGDTR
                     FIAVFYSNLYNIERVGPVRSGRYFDENITRMYHSFLMFKGADPREFDYFMGLDIKQRF
                     ISVGIGKCPPYYYGPYKRDSYNNIFFNVTLWEACVAKKGLDNGPQTISGGFFSEEVPQ
                     SPLTVNKIYNFYTEYNYS"
     gene            952..>1585
                     /locus_tag="PKI33_14445"
     CDS             952..>1585
                     /locus_tag="PKI33_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38266.1"
                     /translation="MLILVTCLLLFITALVLVILRATQPNARYAWLVAAGGATLAFVS
                     VFVWLAQIPFQLTLPVWKPESLFSTPILFSADGVSWALAISIAALTLAVIFTVVTRSV
                     VFNSLSWAGILVLGGMGILAVTANNPLTLLLMWALLDMTELGTQLSSVSGEKNNERVV
                     ISFATRMIGIGLLLWAYIESFTGGGMVVFQTMPPDTGVYLVIAAGLRLGVL"
BASE COUNT          384 a          331 c          437 g          433 t
ORIGIN      
        1 gctatagttg tattcggtat aaaagttgta gattttatta actgtaagtg gactttgcgg
       61 aacttcttca gagaaaaagc ccccgctgat cgtttgtggt ccgttatcga gccccttttt
      121 ggctacacaa gcttcccaaa gggtaacgtt gaaaaagata ttgttataag agtcgcgttt
      181 atagggacca taatagtacg gcggacattt accaatacct actgagataa aacgttgttt
      241 aatatccaac cccatgaaat agtcgaactc acgcgggtcg gcacctttga acatcaggaa
      301 ggaatgatac atacgcgtga tgttctcatc aaaataacgc cccgagcgga cgggtcccac
      361 ccgctcgatg ttgtagagat tgctatagaa gaccgcaata aaacgggtat ccccccattc
      421 aatgtagtat tcatagacca cgtctgcaag ggtcaagcca gattgcgggc gaacgtaatc
      481 tggggaattc cccactttga tggcgagtgg acgatgttgc aggaagtatg ggtctgatac
      541 tgggagcccg gtcaacgggt tgtagagggt cacaccgggt tgcgcagcag gcaatacatc
      601 tacagggacg gatacatctt gaggcggaac atatttggtt gggacaggtg tatatgtagg
      661 gacggcgtct tgcggggtgc tgatggcaag ataagggtct ggagatgtgc ccggctgagg
      721 ttgaaaaggt gtcggcgtag gttgataatt aacttgaaga ggaacaagcg tcgggtcggg
      781 ggttgaggcg ggggcaggct ctgcacaaga aacggcgagg aagagggtga aaagccagag
      841 atttgttcca tgccgatatg gtcggtgact tccaaaattc atggcaggga ttgtaccaaa
      901 ccacaaggta tcagcgcgac aatcataata ctgttacaat tatgttttcc catgttgatc
      961 ttggttacct gtcttttgct ttttattaca gcacttgtgc tggtgatttt acgcgctacc
     1021 caacccaatg cacgctatgc ctggttggtg gcagccggtg gtgccacgct tgcgttcgtg
     1081 agtgtatttg tatggcttgc tcaaattccc tttcaactaa cattgccggt ctggaaacct
     1141 gagagtttat tttctacacc cattcttttt agtgctgatg gggtctcttg ggcgttagcg
     1201 atcagcatcg cggcgttgac tcttgcggtg atcttcacgg tcgtgacacg ctcagtcgtc
     1261 ttcaactcgc tttcatgggc tgggattctt gtgctaggtg gcatgggtat tttggcagta
     1321 accgccaata acccactgac gttactctta atgtgggctc tcctcgacat gacagagtta
     1381 ggcacacaat taagctcagt gagcggcgag aaaaataatg aacgtgtcgt gatctcgttc
     1441 gcaacacgca tgatcggtat cggtctatta ttgtgggcat atattgagag ttttacgggc
     1501 ggcggaatgg ttgtttttca aacaatgcca cctgatacgg gtgtttatct ggtcattgca
     1561 gcgggtttaa ggcttggcgt attgc
//
LOCUS       DAOREF010000806         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_71624_length_1584_cov_3.090255, whole genome shotgun sequence.
ACCESSION   DAOREF010000806 DAOREF010000000
VERSION     DAOREF010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71624_length_1584_cov_3.090255"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKI33_14450"
     CDS             <1..636
                     /locus_tag="PKI33_14450"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNM38267.1"
                     /translation="FCGPFLFKNGLVTVHPQTAMTMNNGVIESRPLYTDKDPQADTLE
                     VGTLIMKIIVRGDATLVRVWDKEAELKKGFKRFNYYEPNPKFKVTAKYVRYDPPKPTI
                     RIEGIGTEVQTFFMGEAQFTLNGKDYSLQAEESGDELLFNFKDETNTDTTYGGGRRFY
                     LLPPEGDELILDFNLTDNWPCAYTPFATCPIPPKENTLKVRIEAGEKKFKK"
     gene            complement(670..1230)
                     /locus_tag="PKI33_14455"
     CDS             complement(670..1230)
                     /locus_tag="PKI33_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNM38268.1"
                     /translation="MELFEAIHGRTTVSKVKQDALSRETIEKLLSAGAQAPNHHKVRP
                     WRFVVLTGDGRKKLGDVMTASFADRNPGAPAEGLDKTRNLPLRAPVVIAVGADKPADA
                     KIQEIENISAASAACQNILLAAHALGLGAIWRTGEWARDVKVKEFLGFAPDQHIVGFI
                     YIGNPDIMPDPYTRIGFEDRVTWIDK"
     gene            1278..>1584
                     /locus_tag="PKI33_14460"
     CDS             1278..>1584
                     /locus_tag="PKI33_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38269.1"
                     /translation="MKIFYSEEHRNHYPPFEVFDGGTRVQYFENPDRMDQILNALQGE
                     NWVTFLKPDDFGLDPILAVHDRDYLNFLASCWDEWLDSDPEVAASPETHAFLPATFAL
                     "
BASE COUNT          434 a          397 c          347 g          406 t
ORIGIN      
        1 ttttgcggtc ccttcctctt caaaaatgga cttgtcacag tacatccgca aacagcgatg
       61 accatgaaca atggcgtgat agagtctcgc cctttatata cagacaaaga cccacaagcc
      121 gatacgcttg aagtggggac actcattatg aaaattattg tgcgtggaga tgcgactctt
      181 gtacgcgtat gggacaagga agcagaactc aagaaaggct tcaaacgctt taactactac
      241 gagcccaacc caaagtttaa ggtaacagcc aagtatgttc gctacgaccc gcccaagcca
      301 acgattcgaa tagaaggcat tggtaccgaa gtgcaaactt tctttatggg agaagcccaa
      361 ttcaccttga acggaaaaga ctattcactt caagccgaag aaagtggcga cgaattgctc
      421 tttaatttca aggatgaaac caatacagat acgacttatg gtggtggacg caggttttat
      481 cttctgccgc cagaaggcga tgaactcatt ttagatttca acttaactga caattggccc
      541 tgcgcgtaca caccttttgc cacctgccct attccgccca aagaaaacac attgaaggtc
      601 cgcatagaag ctggcgagaa gaaatttaag aagtaaacaa aaagcgagtg gcaagccact
      661 cgctttttgt tatttatcaa tccatgttac acgatcttca aacccaattc tagtatatgg
      721 gtctggcatt atgtctgggt tcccaatata aataaaacca acaatatgct gatcgggcgc
      781 aaagccaagg aattctttta ctttcacatc gcgtgcccat tcgccggtgc gccaaatagc
      841 gcccaagcct aaagcatgtg ccgctaacaa aatattctgg caagctgctg aagccgcaga
      901 aatgttttca atttcttgga tctttgcatc agcaggttta tctgcgccaa cggcaattac
      961 aaccggtgcg cggagcggca agtttcgggt tttatccaag ccttcggctg gcgcacccgg
     1021 gttccggtcg gcgaaagagg cggtcattac atctcctaac ttcttacgcc catccccggt
     1081 taacacaaca aaacgccagg gtctcacttt gtgatgattc ggcgcttgtg caccggcact
     1141 taataatttt tcaatggtct cgcgtgaaag tgcatcttgc ttcactttgc tgaccgttgt
     1201 tcgtccgtga attgcttcaa aaagttccat ttcgatacct ccgggagtga gacgcttgca
     1261 ggtaaaattg tcttaccatg aaaatatttt actcagaaga acaccgcaat cattatccac
     1321 ctttcgaagt gttcgatggc ggcacaaggg tgcaatattt tgaaaaccca gaccgcatgg
     1381 atcaaatcct aaatgcgcta cagggtgaaa actgggtgac atttttaaag ccagatgatt
     1441 ttggactcga cccgatcctt gctgtgcacg accgtgacta cctcaacttc ctcgcctcct
     1501 gctgggacga gtggctagac tctgaccctg aagttgctgc ttcgccggaa acgcatgctt
     1561 tcttgcccgc tacctttgct ttgc
//
LOCUS       DAOREF010000807         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_72008_length_1580_cov_3.693770, whole genome shotgun sequence.
ACCESSION   DAOREF010000807 DAOREF010000000
VERSION     DAOREF010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72008_length_1580_cov_3.693770"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKI33_14465"
     CDS             <1..897
                     /locus_tag="PKI33_14465"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNM38270.1"
                     /translation="AETAASGLMHNPTNDLIYWIEVNPRGERLSLNAAPLRVGPLVQK
                     HLWYEKASVILASATLTTHGEFTYIRNTLDAAEVDTLALGSPFDYESSTLLYVPSDMP
                     EPNAFNYQQILDRSIIATAKATGGRMLVLFTSYAALKKTSQAITGALAREDIFVFEQG
                     EGTSPTALLESFKTTDRAVLLGTRSFWEGVDVPGDSLSVVMITKLPFEVPSDPIISAR
                     SELYEDSFNEYYLPEAILKFRQGFGRLIRTASDRGIVAILDRRVLTKQYGRMFLESLP
                     QCTARQGPIANLPKMAGQWLGM"
     gene            968..>1580
                     /locus_tag="PKI33_14470"
     CDS             968..>1580
                     /locus_tag="PKI33_14470"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNM38271.1"
                     /translation="MPNIYFTLFEIIIYFQFVLCLNHARKQGAANLLKLAFGVLFGVS
                     LELATIRQLHAYEYGQFLLMVLDVPLCIGVAWSCIIYAAMEFSDASSLPYFFRPVLDG
                     LLALNIDLALDAVAIRFGFWDWGQGLTTQYFGVPYANFWAWFWVVFSFSLGYRLLAKN
                     TTWVGTWLSAPLAYLIGLVGVIGTNALIVFVVPTTIRPGVIGLT"
BASE COUNT          357 a          457 c          352 g          414 t
ORIGIN      
        1 gcggaaaccg ccgcctctgg cttgatgcac aaccccacca acgacttgat ctactggatc
       61 gaagtcaacc cgcgtggaga acgcctttcc ttaaacgccg ccccgcttcg ggtaggacct
      121 ttagtacaaa aacatctctg gtacgaaaaa gccagtgtga ttctcgcttc ggcaactctc
      181 accactcatg gcgagtttac ctacatccgc aacacactcg acgcagccga agtggatacg
      241 ctcgcgctcg gctccccttt tgactatgag tccagcacct tgctctatgt gcctagtgac
      301 atgcccgagc caaatgcgtt caattaccag caaattctcg accgttccat catcgccacc
      361 gccaaagcca cgggcggacg aatgcttgtg ctgtttacat cctatgctgc tttgaagaaa
      421 acctcgcaag ccattacagg cgctctggca agagaagata tttttgtctt tgaacaaggc
      481 gaagggactt cgcccacagc cttgctcgag tccttcaaaa ctacagaccg tgccgtgctc
      541 ttgggaactc gctctttctg ggaaggcgtg gacgtgcccg gtgattcgct ctctgtggtg
      601 atgatcacca aattaccctt tgaagtgccg tcagacccca ttatttcggc acgctcagaa
      661 ctgtacgaag attccttcaa cgaatattac ctgcctgaag ccattctcaa attccggcag
      721 ggatttggcc gcctcatccg caccgcatca gatcgcggca ttgtcgccat tctcgaccgt
      781 cgtgtgctca caaaacaata tggcagaatg ttcttggaat ctctgcccca atgtacagcc
      841 cggcaaggac caattgccaa tctaccaaag atggcaggtc agtggctggg aatgtaaaat
      901 aagaaatatc aatacgtgac agtcatctta aagatgactg tcactttaat aactcaaggt
      961 ccaagccatg cccaatatct acttcaccct cttcgaaatt atcatctatt ttcaattcgt
     1021 cctctgcctt aatcacgctc gcaaacaggg cgctgcaaat ttactcaaac ttgcgtttgg
     1081 cgttctattt ggagtctccc tcgaactcgc taccattagg caactgcatg cttatgaata
     1141 cggtcaattc ctgctcatgg tcttagatgt gcctctttgc attggcgtgg catggagttg
     1201 catcatttac gccgccatgg agttttcaga cgccagcagt ctgccctact tcttccgccc
     1261 ggtattagac ggtctcctcg ccctcaacat tgacctcgcg ctcgacgcgg tagccatccg
     1321 cttcgggttc tgggattggg ggcaagggct caccacacaa tacttcggcg taccttatgc
     1381 aaacttctgg gcttggtttt gggtcgtctt ttccttctcg cttggctacc gcctgctggc
     1441 aaaaaatacc acttgggtcg gtacctggct ctctgctccg ttggcatacc tcattgggtt
     1501 agtaggagtt atcgggacaa atgccctaat cgtattcgtt gttcctacga ctattcgccc
     1561 tggtgtaatc ggattgactc
//
LOCUS       DAOREF010000808         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_72476_length_1575_cov_3.326974, whole genome shotgun sequence.
ACCESSION   DAOREF010000808 DAOREF010000000
VERSION     DAOREF010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72476_length_1575_cov_3.326974"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKI33_14475"
     CDS             <1..730
                     /locus_tag="PKI33_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNM38272.1"
                     /translation="MKATVRGQGGHGSMPEKGGATAKLATFLRALDEKDLPVHVTPAT
                     KLMVDAMASALGGLQGFILAQLTNPALTDLVLKTLGSRVRSFYPLFRNTASPTIIHSS
                     DKVNVIPSEISVELDGRLLPGQKPEDMIRELKAIAGDDVEFELIQYEPGPAEPDMTLF
                     NTLADILKEADPDGIPIPLILSGVTDGRFFSQIGIQTYGYLPMTLPEDFNFADVIHAA
                     DERVPAAAIEFGTQAIYKALSRFG"
     gene            745..1227
                     /locus_tag="PKI33_14480"
     CDS             745..1227
                     /locus_tag="PKI33_14480"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNM38273.1"
                     /translation="MNYKRIPMDHDKKIALVAHDNKKRDLLEWAKFNRDLLAHHQIYA
                     TGTTGKILEKELNLKVTKLKSGPLGGDQQIGAKIVENKIDFLIFFWDPLEPQPHDPDV
                     KALLRMAVVWNIPIANNRASADFMISSPLMDNPYERLVPDYETYTSRKIDLGDKSGKV
                     "
     gene            1230..>1575
                     /locus_tag="PKI33_14485"
     CDS             1230..>1575
                     /locus_tag="PKI33_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38274.1"
                     /translation="MRIVLVGVLLFCITACGLIPTAPLAPIVADLDSEFTLVPGQSAT
                     INGTDLKITLLSVPGDQRCPLNIECAASGPVSVAISVQSNSGTPQEADFMTVTDNDGK
                     VPEMEFEGMTTRI"
BASE COUNT          415 a          359 c          400 g          401 t
ORIGIN      
        1 gatgaaagcc accgtgcgcg gacagggtgg tcacggttcg atgccagaga aaggtggcgc
       61 aactgccaaa ctggcaacgt tcttaagggc gttggatgaa aaagacttgc ctgtacatgt
      121 gaccccggca acaaaattga tggtagatgc catggcctcg gctctgggcg gacttcaagg
      181 ttttatcctc gctcagctaa ccaaccctgc gctgacggat cttgtgctca aaacccttgg
      241 ttctcgggtg cgttcgttct atccattgtt taggaatact gccagcccaa cgattattca
      301 tagcagtgat aaggtgaatg taattccgag tgaaatttct gtggaattgg atggacgttt
      361 gttaccgggt caaaagcctg aagatatgat ccgtgaattg aaagccatag ccggtgacga
      421 tgtagagttc gaactgattc aatatgaacc gggtcctgct gaaccggata tgacgctttt
      481 caacaccttg gcagatatct tgaaagaagc cgaccctgat gggattccga ttccgttgat
      541 tcttagtggt gtaaccgatg gtcgcttctt ctctcagatt ggtattcaaa cctacggcta
      601 tttgcccatg actttgccag aagacttcaa cttcgcagat gtgatccatg ccgcagatga
      661 acgcgtgcca gccgccgcaa ttgagtttgg cacacaggcg atctataaag cgttaagtcg
      721 ttttgggtaa tgtaaggaga cagaatgaat tacaagcgta ttccaatgga tcatgacaaa
      781 aagatcgcat tggtggcaca tgataataaa aaacgagact tgctcgaatg ggcaaagttt
      841 aatcgtgatt tattagccca tcaccaaatc tatgctacag gcaccactgg caagattttg
      901 gagaaagaac taaatctcaa agtgaccaag ctcaaaagcg gaccgctcgg cggcgaccag
      961 caaattggtg ccaagatcgt tgagaacaag attgacttcc ttattttctt ttgggacccg
     1021 ttggagccgc agccacatga ccccgatgtg aaagcgctct tgcgcatggc ggtggtgtgg
     1081 aatatcccga tcgcgaacaa tcgcgcttcg gcagatttta tgatctcgtc accattaatg
     1141 gacaatccat acgagcgctt ggtgccagat tatgaaacct acacctcgcg aaaaattgac
     1201 ctaggggata agagcgggaa ggtatgagga tgagaatcgt tcttgtgggc gttcttctct
     1261 tttgcataac cgcgtgcgga ctgataccaa cggctcctct tgcgcctatt gttgctgacc
     1321 ttgattctga attcaccttg gtcccagggc aatctgcgac cataaacgga acagacttga
     1381 agattacact gctaagtgtt cccggagacc agcgttgtcc cctcaatatt gagtgcgccg
     1441 ctagtggacc tgtttcagtg gcaatttccg ttcaatcaaa ttctggaacg cctcaagaag
     1501 cggattttat gaccgtcact gataacgatg gcaaggtgcc tgagatggaa tttgagggaa
     1561 tgacaactcg tattg
//
LOCUS       DAOREF010000809         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_72739_length_1572_cov_3.866183, whole genome shotgun sequence.
ACCESSION   DAOREF010000809 DAOREF010000000
VERSION     DAOREF010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72739_length_1572_cov_3.866183"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKI33_14490"
     CDS             <1..914
                     /locus_tag="PKI33_14490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNM38275.1"
                     /translation="FPLNPQSDLSELKRYKDSGVTFVSLNIGMDMDSFENVMRVLARF
                     RSHIAAHPNELILARSVEDIHRAKRENKLAIAFDLEGSNPLCGDLNLVSFYYDLGVRQ
                     MLLAYNKDNAASGGCMEGRIGLTEFGRQVVQEMNRVGMVVDVTHMGYRATMDTFETSS
                     APVIFSHSNPASLHAHARNISDEQIKACAKTGGVIAINGIGDFLGGKTSELFVQHLEY
                     VLNLVGPEHVGIGLDYVIDKQELIDYIQGNPDVFPADRFEDFLSFVEPEQLPEISEIL
                     YKHGHDEDVIRKVLGGNFLRVAEQTWK"
     gene            942..>1572
                     /locus_tag="PKI33_14495"
     CDS             942..>1572
                     /locus_tag="PKI33_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38276.1"
                     /translation="MSTILLTGGRAPVTLDLARAFQRAGHVVYVAESLRGHLTEPSAA
                     LKAHFEVPAPRQEPEAFLAALRSIIEAYQIELLIPTCEEVFHIAKGLNTLPCRVFTEP
                     LARLDGYHNKWKFVVKAGECDLRVPESMLVTRQDDLLYAYAHWRGLVLKPVYSRFAAR
                     TLILPSLKDAMATLTFDSLTNQEPWIAQEYIEGQQFCSYSVCQNGRILAH"
BASE COUNT          366 a          369 c          442 g          395 t
ORIGIN      
        1 gttttccgtt gaatccacaa tcggatctga gcgagctgaa acgctacaaa gacagcggtg
       61 tgaccttcgt gtcgctcaac atcggcatgg acatggactc gttcgagaat gtgatgcgcg
      121 tgttggcacg ttttcgctcc catattgcgg ctcatcccaa tgaattgatt ctggcacggt
      181 ctgtggaaga tattcaccgc gccaaacgag agaataaatt agccatcgct tttgatctgg
      241 aagggtcgaa cccgttatgc ggtgacctga acctagtctc gttctattat gatcttggtg
      301 tgcgccaaat gctattggca tacaacaaag acaatgccgc ttcaggcggg tgcatggagg
      361 ggagaatcgg gctgacggag ttcggcaggc aagtggtgca agagatgaac cgtgtgggca
      421 tggtggtgga tgtaacacac atgggctacc gcgccacgat ggataccttt gagacctcta
      481 gtgcgccggt catcttttca cattcgaacc cagccagctt acacgcccat gcccgaaata
      541 tttcagatga gcagatcaaa gcgtgtgcca agaccggcgg cgtgatcgcc atcaacggca
      601 ttggcgattt tttaggcggc aaaaccagcg agctttttgt acaacacctt gagtatgttt
      661 tgaatttggt cggacccgaa catgtgggaa ttgggttgga ttatgtcatc gacaaacagg
      721 aacttatcga ctacattcag ggaaatccgg atgtcttccc agccgaccgc tttgaagatt
      781 tcctttcgtt cgtagaacca gaacaattgc cagagatcag cgagatttta tacaagcacg
      841 ggcatgatga agatgtgatc cgtaaggtgt tgggtgggaa cttcttgcgg gtggcagaac
      901 aaacctggaa gtaaaaaatc ccttattggc aggctgacct aatgtcaacc attcttctca
      961 ccggcgggcg agcccccgtc accttagatt tggcgcgtgc gtttcagcga gcggggcatg
     1021 tggtgtatgt tgccgagtct ttgcgcgggc atctcaccga accttcggcg gcgctcaagg
     1081 ctcattttga ggtgccagct ccacgtcagg aacctgaggc gtttttagcg gcgttgcgaa
     1141 gcatcatcga agcgtatcag atcgagttgc tgattcccac ctgtgaggaa gttttccata
     1201 ttgcaaaagg gttgaatact ttaccctgcc gcgtgtttac cgagccgctg gcaaggttgg
     1261 atggatatca caacaagtgg aagtttgtgg tgaaggcggg agaatgcgat ttgcgcgtgc
     1321 cagagtcgat gttggtgacg agacaagatg acttgctcta tgcctacgct cactggcgcg
     1381 gactggtgct gaagccggtc tattcccgct tcgctgcgcg gaccttgatc ttgccctcgt
     1441 taaaagatgc tatggctacg ctgacgtttg actctctcac gaaccaagag ccgtggatcg
     1501 cacaagaata cattgaggga cagcaatttt gtagttattc ggtttgccag aatgggcgta
     1561 ttttggcgca tt
//
LOCUS       DAOREF010000810         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_72748_length_1572_cov_2.915623, whole genome shotgun sequence.
ACCESSION   DAOREF010000810 DAOREF010000000
VERSION     DAOREF010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72748_length_1572_cov_2.915623"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKI33_14500"
     CDS             complement(<1..379)
                     /locus_tag="PKI33_14500"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNM38277.1"
                     /translation="MRIGRILFVVVLALCLFEMARLWTISPLQMAAHFNALGEPDRFV
                     PKSEFFWGQVQTLFVVVLVSLPLQVLFLVIPPNLINMPNREYWLAAERKEETLGRLSD
                     FGSMMFAVILLALLAGFEIATYAN"
     gene            complement(388..615)
                     /locus_tag="PKI33_14505"
     CDS             complement(388..615)
                     /locus_tag="PKI33_14505"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNM38278.1"
                     /translation="MTETSQPNNPLHGITLETILTQLVQQYGWAELGKRIPIKCFTND
                     PSVKSSLKFLRQTPWARKKVEDLYLQSVGKG"
     gene            complement(639..1058)
                     /locus_tag="PKI33_14510"
     CDS             complement(639..1058)
                     /locus_tag="PKI33_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38279.1"
                     /translation="MKPKMMLGCLLVGLTLLVGCGPKETPLSGPERDAVLAFSEAKTD
                     NLLAGMNASDYAAFSQDFDQEMLAAIPQSAFETLKADRDATLGLYVSRTVNTVVQSGE
                     FYAVVYDAVFEKDEAVTVRVVFRVAEPHQVSGLWFNK"
     gene            complement(1101..>1572)
                     /locus_tag="PKI33_14515"
     CDS             complement(1101..>1572)
                     /locus_tag="PKI33_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38280.1"
                     /translation="NFYFENNKPAQAAPIFAKLAEVLRSAGQPKRAASLHSQAALAFA
                     RSRNESALVQARAAMSLLLQYKLEAQASAFYKELMREFTKRGMQPAAEAFAKEFGGQI
                     QQPAEQDAHAAHQLDRLPSHCPSCGASLRERDIRWTDADTIECGFCGTPIRPSV"
BASE COUNT          394 a          445 c          383 g          350 t
ORIGIN      
        1 aattggcata ggtggcgatc tcaaaaccag ccagaagcgc aagcaggatc acggcgaaca
       61 tcatcgagcc gaaatcgctc aaacgcccga gggtttcttc tttccgttcg gcagccaacc
      121 aatactcgcg gttgggcatg ttgatcaagt tgggtggtat gaccagaaac aagacctgca
      181 agggcaagct gaccaacaca acgacaaaca aggtttgtac ctgcccccaa aagaattcgg
      241 acttgggcac aaagcggtct ggctcaccca aggcattgaa atgcgccgcc atctgcagcg
      301 gactgatcgt ccacaagcgc gccatctcga agagacagag cgccagcaca actacaaaaa
      361 gaattcgccc gatacgcatg gggcacctta gcctttgccg acggattgca ggtacaagtc
      421 ttccactttc tttctcgccc acggcgtttg cctcaaaaac ttgaggctcg atttgacgct
      481 ggggtcgttc gtaaagcatt tgatcggaat gcgcttgccc aactctgccc aaccgtattg
      541 ttgtacgagt tgagtaagaa tcgtttcaag cgtaatgcca tgtaaaggat tattgggttg
      601 tgaagtttct gtcataacaa ttccagtgta cgctagagct acttgttaaa ccataacccg
      661 ctgacctgat gcggttctgc cacacggaag accactcgca ccgtaacagc ctcatccttt
      721 tcaaaaacag catcatagac caccgcataa aactctccac tttgcaccac agtgttgacc
      781 gtgcgtgaaa cgtataaccc taatgtagca tcgcggtctg ccttcaaggt ctcgaaagcg
      841 ctttgcggaa tggcagccaa catctcttgg tcaaagtctt gcgagaaggc ggcataatca
      901 ctggcattca tccctgccag cagattgtcc gtcttggctt cgctaaaggc gaggacggca
      961 tcccgttccg gtccagagag aggcgtttct ttcggtccac acccaaccag caaggtgaga
     1021 cccaccaaca ggcatccaag catcattttt ggtttcatgg caattcatca tcctttttta
     1081 taaaaccgct atattacgtt ttaaacagac gggcgaatgg gcgtgccgca aaagccacat
     1141 tcgatggtgt ctgcatccgt ccagcggata tccctctcac gcagagacgc cccgcaactg
     1201 ggacaatgac taggcaggcg gtctagctga tgagccgcat gcgcatcttg ctccgccggt
     1261 tgctgaatct gcccaccgaa ctctttggca aacgcctcag ccgcaggttg cattccccgc
     1321 ttggtgaatt cacgcatcaa ctccttataa aacgcggacg cttgcgcttc gagcttatat
     1381 tgcagcaaca aagacattgc cgcccgagcc tgcaccagcg cggactcatt tcggctgcgg
     1441 gcaaaggcga gcgcggcttg agaatgtaaa cttgccgcac gcttgggctg cccggcagaa
     1501 cgcaacacct ctgccagctt ggcaaagatc ggtgccgctt gggctggctt gttgttctca
     1561 aaatagaaat ta
//
LOCUS       DAOREF010000811         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_72833_length_1571_cov_4.387203, whole genome shotgun sequence.
ACCESSION   DAOREF010000811 DAOREF010000000
VERSION     DAOREF010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72833_length_1571_cov_4.387203"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PKI33_14520"
     CDS             <1..934
                     /locus_tag="PKI33_14520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM38281.1"
                     /translation="ILAGIALVFGVGGYSSFSTPFLLSTSLFIVGLGLGAFELGPNAV
                     IVSLYHEQKGLYLNLMAVMHGLGSMIAPLVAGYLLSMDVNWRTIYRFDLLLIAIFLVA
                     SLLVRFPKSEEKASLDFRAIPTVAFKHTLPFYYGAMLFYVAAEIGLASWMVVYLQEAR
                     SLNETASNNYLAIFFGMLMLGRFFGSFFVKRVGYLRSILFATLLATASIALGIFTNLV
                     IFIPITGFFFSIIFPTLTAAVSDAEQENINTILGTLFTFSGIGGMIGPWLVAWSSKLF
                     GLQVGFSSIILLTGSTLVFAFILNQRSNTNGQRS"
     gene            918..>1571
                     /locus_tag="PKI33_14525"
     CDS             918..>1571
                     /locus_tag="PKI33_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNM38282.1"
                     /translation="MDNVLNWGLLSTAKINRALIKPLRASKRTRLLGVASRSQSSADE
                     YAREWKIPRAYGSYESMLADPEIDIVYNSLPNHLHEEWTVKALRAGKHVLCEKPLALT
                     VEEVDRIIAASNETGKVAAEAFMYRHHAQTLKIKELVDSNLIGQIQLIKGAFTFTLNR
                     EGNYRFIKEYGGGSLWDVGCYPISFARMVVGEEPVEVFGSQVLGPGGSDDSFYGQMRF
                     "
BASE COUNT          367 a          387 c          357 g          460 t
ORIGIN      
        1 cattcttgcg gggatagctc tcgttttcgg agttggcgga tacagttcat tcagtacacc
       61 gttcctgtta tcaacctctc tctttatcgt tgggcttggt ctgggagcgt ttgaacttgg
      121 tcccaacgcc gttattgtca gtttgtatca tgaacaaaaa ggcttatatc ttaatctcat
      181 ggcggtcatg cacggacttg gctccatgat cgcgccttta gttgcaggat acctcctaag
      241 tatggatgtc aactggcgga cgatctatcg ctttgattta ctgctcatcg caatttttct
      301 tgttgccagc ctgttagtac gctttccgaa gtcagaagaa aaagcctcgt tagattttcg
      361 cgccatccca acggttgcat tcaaacacac attgccgttt tactatggag ccatgctatt
      421 ttatgttgca gccgaaattg gtttggcatc ctggatggtc gtctatttac aagaagcacg
      481 cagcctaaac gaaacagcaa gcaataatta tctcgccatc ttttttggca tgctgatgtt
      541 gggacgtttc ttcggcagtt tctttgtaaa acgcgtcggc tatttgcgct ctattttatt
      601 tgccacactt cttgccacgg caagcatcgc gcttggcatt tttaccaacc ttgtgatttt
      661 cattccaatc actggctttt tcttttcaat catcttcccc accctcaccg ccgccgtttc
      721 tgatgctgaa caagaaaaca tcaataccat tctcggtacg ctatttacct tttctggcat
      781 cggcgggatg atcggtccat ggttggtggc gtggagcagt aaactcttcg gtttgcaagt
      841 cggtttttca tccatcattc tattgacagg ctcaaccttg gtctttgcct tcattctcaa
      901 ccaaaggagt aacacaaatg gacaacgttc ttaattgggg attgctctcc accgccaaaa
      961 tcaaccgcgc tttgattaaa cccctgcgcg cctccaaacg gacccgctta ctgggtgtcg
     1021 cttcgagaag ccaatcctcc gctgacgaat atgcccgcga atggaaaatc cctcgtgctt
     1081 atggcagtta tgagtccatg ctcgctgacc ctgagatcga tattgtctac aactctttgc
     1141 ccaatcacct gcacgaagag tggacagtaa aagctttgcg agcagggaaa catgttttat
     1201 gcgaaaaacc actggctctt actgtagaag aagtagaccg catcattgct gcctcgaacg
     1261 aaacaggcaa ggtcgccgcc gaagcgttca tgtatcgtca tcatgcacag acactaaaaa
     1321 taaaagaatt ggtagatagc aacttgattg gacaaattca gcttatcaaa ggcgctttta
     1381 cgtttactct caatcgtgag ggaaattatc gcttcatcaa agaatacggc ggtggcagtt
     1441 tatgggatgt gggctgttac ccgatctcgt tcgcgcgaat ggttgtcggc gaggaaccgg
     1501 ttgaggtatt tggctcacag gttctcggtc cgggaggaag cgacgactcg ttctacgggc
     1561 agatgcgttt c
//
LOCUS       DAOREF010000812         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_73262_length_1567_cov_2.013889, whole genome shotgun sequence.
ACCESSION   DAOREF010000812 DAOREF010000000
VERSION     DAOREF010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73262_length_1567_cov_2.013889"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKI33_14530"
     CDS             <1..410
                     /locus_tag="PKI33_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38283.1"
                     /translation="KWLTACLILLTSAYQFTQTDAKTDDHHFKGFPSYWNVAALYMLL
                     MNLPPWVNFGFLMLFNIMVFIPVKYIYPSRNSYLRTLTLAFTYLYGAVGIWGLLLYPN
                     TPSWIVWASFLYVGYYLVLSLIPKSKNTTAHAN"
     gene            400..843
                     /locus_tag="PKI33_14535"
     CDS             400..843
                     /locus_tag="PKI33_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM38284.1"
                     /translation="MQIEIATRADDELYEAFQRLTPQLTQNNPPPTREDLSALINEPS
                     STLLLARNELGQVVGALNLTVYRVPTGIRAIIEDVIVDLSARGQGIGEAIMQQAIEIA
                     QQKGASNISLTSNPMRVAANKLYIKLGFEKRETNAYQMKFKKAAE"
     gene            complement(878..>1567)
                     /locus_tag="PKI33_14540"
     CDS             complement(878..>1567)
                     /locus_tag="PKI33_14540"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNM38285.1"
                     /translation="PVDSFDMARQDPKEQIPSPWSCQPTDADLLNSLAMDFARNKFSL
                     KALMRTITRSSAYQLSARFDGEWKESYTPYFARHFVKQLTAEQLHDSICQATDVFGNY
                     KRNDWTYETPLSPVKYWTETTGPEGVGTAEAKNFMRTFGQSNREQFDRQSGGSILQAM
                     SLMNSPFVNKKVLAENGSRVEKLVKSQMSNQEIVDEMMYRIASMMPKEYHGEYSDFSK
                     STGKYLVNTNL"
BASE COUNT          394 a          406 c          330 g          437 t
ORIGIN      
        1 tcaaatggct tacggcttgt ctcatcctgc tcacgtccgc gtatcaattc acccaaaccg
       61 atgccaagac cgatgaccat cacttcaaag gtttcccttc ctattggaat gttgctgcgc
      121 tgtatatgct gcttatgaat ttgccgccct gggtcaattt cggttttctg atgctcttca
      181 acatcatggt tttcatcccg gtcaagtata tttaccccag ccgcaatagt tatctccgta
      241 ccctcacact ggcgttcacg tatctctatg gcgctgtggg tatctggggc ttgctgcttt
      301 atcccaatac cccatcttgg atcgtctggg catcctttct ctatgtaggc tattatctcg
      361 tgttgagcct aattccaaag agcaagaaca caaccgccca tgcaaattga aatcgccacc
      421 cgagccgatg atgaactcta tgaggcgttt caacgtctca cgccgcagtt gactcaaaat
      481 aatccgcctc ctacgcgcga agacctgtct gcgcttatca atgaaccttc gtctacgctg
      541 ttgcttgccc gcaacgaact cggacaggtc gtcggcgccc taaaccttac cgtttatcgc
      601 gtgccaactg gcatccgcgc catcatcgaa gatgtcatcg tggacttgtc tgctcggggg
      661 caggggattg gtgaagccat catgcaacaa gcaattgaga ttgcgcaaca aaaaggcgcc
      721 agcaatatct cgctaacctc aaatccaatg cgagtggcgg caaacaaatt gtatattaag
      781 ttgggctttg aaaaacgcga aacaaatgcc taccaaatga aattcaagaa agctgctgaa
      841 taaataaaaa ccggctcttc tttgagccgg tttttattta tagatttgta ttaaccagat
      901 atttccctgt tgatttgcta aagtcgctat attcgccgtg atattctttt ggcatcatgg
      961 atgcgatccg atacatcatt tcatccacga tctcttgatt agacatctgg gacttcacta
     1021 atttctcaac tcgactaccg ttctctgcta agactttttt atttacaaac gggctattca
     1081 ttaaagacat cgcctgtaag atcgaccctc cagattgacg atcaaactgc tcccggttgg
     1141 actggccaaa ggtacgcata aagtttttgg cctcggcggt gccgacgcct tcggggccag
     1201 tggtttccgt ccaatactta acaggagaca agggggtttc ataggtccaa tcatttcgct
     1261 tatagttccc aaacacatcg gttgcctggc agatcgaatc gtgcaattgc tccgcggtca
     1321 attgcttcac aaaatgacgt gcgaagtaag gtgtataact ctctttccac tctccatcaa
     1381 aacgcgcaga tagttgatat gcactggagc gggttatcgt tcgcatcaag gccttcaaac
     1441 taaatttatt cctagcgaag tccatagcta aagaattcag caaatctgca tctgtaggct
     1501 ggcaactcca gggagacgga atctgttctt tagggtcttg ccttgccata tcaaatgaat
     1561 ccacagg
//
LOCUS       DAOREF010000813         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_73777_length_1561_cov_2.594290, whole genome shotgun sequence.
ACCESSION   DAOREF010000813 DAOREF010000000
VERSION     DAOREF010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73777_length_1561_cov_2.594290"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKI33_14545"
     CDS             complement(<1..1212)
                     /locus_tag="PKI33_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM38286.1"
                     /translation="MKLKLFTMLVILGLVLSACSSGGNAQATESTSGGGGGGTVTLII
                     PEEPTTLNFYLSDAAIVRQVADSTSMTGLVTIDESGNYVPMLADGLPTISDDHLTVTW
                     KLKDGLKWSDGEALTSDDVRFTVEVLSNPDSGALVGTSGFDMISSVETPDDLTTVLTY
                     SEPYPGYLDQFAYGLFPRHATGAPEEMSSWDWNRAPVTAGPFILSNWESGVSITLTRN
                     PYYFEEGKPYLDSIVFAIVPEPAAQTAMMMNGEAQVHLWPGEFKADYDSMLAGKAQQY
                     LIPGIWNMAIDFNLSAPFDGDPSAAAPHPILGDIRVREAIASAINYQALQQDVLQGSV
                     GDSTNPFAYGWYECKLPRKYGYDVEAANKLLDEAGWVMGDDGIRVAKGAMYAKDGTRL
                     SLELQGYTAFDP"
     gene            complement(1239..>1561)
                     /locus_tag="PKI33_14550"
     CDS             complement(1239..>1561)
                     /locus_tag="PKI33_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38287.1"
                     /translation="MGGSDLRIIVRHILPNCVGPILVSGTLQMASAIITESGLSYLGF
                     GVQPPTPTWGSILSTAQNQVFRAPWLAFYPGLMIFVTVMAINYIGDGLRDAFDPYVIH
                     TKSQ"
BASE COUNT          410 a          403 c          373 g          375 t
ORIGIN      
        1 aggatcgaaa gcggtatacc cctgaagttc gagagagagg cgggttccat ctttggcata
       61 catggcacct tttgccacgc ggatgccatc atcgcccatc acccaacccg cttcatccaa
      121 taatttattg gctgcttcca catcataccc atacttgcgc ggaagtttac attcatacca
      181 accataggcg aatggattgg tcgaatcacc gacactgccc tgcaatacat cctgctgcaa
      241 ggcttggtaa ttgatggcgc ttgcaatcgc ttcgcggacg cgaatatcac ccaagatggg
      301 gtgtggagcc gctgcactcg gatcgccatc gaacggtgcg ctcaagttga aatcgatcgc
      361 catattccaa atgccaggaa tgagatattg ctgagctttg ccagccaaca tgctgtcata
      421 gtctgctttg aattcaccgg gccacaaatg aacttgggct tcaccgttca tcatcatagc
      481 ggtttgcgca gcaggttcag gcacaattgc aaacacgatg ctatcgagat agggttttcc
      541 ttcttcaaaa taataagggt tgcgcgtgag ggtaatgctt acgcctgatt cccaattgct
      601 caaaatgaaa ggacctgctg taactggcgc gcgattccaa tcccagctcg acatttcttc
      661 aggtgcaccc gtggcgtggc gggggaacaa tccgtaagcg aattgatcga gatagcccgg
      721 gtaaggttct gaataggtta aaaccgtggt caagtcatct ggcgtttcga cgcttgagat
      781 catgtcaaac ccgctggtgc caaccaacgc cccagaatcg ggattggata acacttcaac
      841 ggtaaagcgg acatcatctg aggtgagtgc ttcaccatca gaccatttca aaccatcttt
      901 caacttccaa gtaacagtga ggtggtcatc tgaaatcgta ggtagcccat ctgctaacat
      961 gggcacataa ttcccagatt cgtcgatggt tactagaccg gtcatcgaag ttgaatctgc
     1021 aacctgtcgg acaatcgcag catctgatag atagaagttc agcgtggtag gctcttctgg
     1081 aatgataagg gtgacggtgc cgcctccacc tccgccgcta gtggattctg ttgcttgggc
     1141 atttccgccg ctggaacaag cactcagaac cagacccaaa ataacaagca ttgtaaacag
     1201 tttgagtttc atctcttctc ctttttgatt tataaactct attgcgattt ggtatggatc
     1261 acatacgggt cgaacgcatc tcttaacccg tcgccaatat agttgatcgc catcacggtg
     1321 acgaagatca tcaagcctgg gtaaaacgcc agccacggcg cacggaagac ttgattttgt
     1381 gcagtggata aaatgctgcc ccatgtaggc gtagggggct gcacaccaaa ccctaaataa
     1441 ctcaagccag actcggtgat gattgccgaa gccatttgca aagttccgct taccaagatc
     1501 ggaccgacac aattcggcaa aatatggcga acaataatgc gcaaatcgct gccacccatg
     1561 g
//
LOCUS       DAOREF010000814         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_74049_length_1558_cov_3.154358, whole genome shotgun sequence.
ACCESSION   DAOREF010000814 DAOREF010000000
VERSION     DAOREF010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74049_length_1558_cov_3.154358"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKI33_14555"
     CDS             <1..826
                     /locus_tag="PKI33_14555"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNM38288.1"
                     /translation="ALFAETYKEVNAQSVIQFMFWEPLNGNSVTSCITKARENARSVR
                     EQISSEMWEAINRLYHLTRNPERAKESNPLEFFKVIRDRAQSYQGVISATMTHGEPYQ
                     FIQLGLHLERADKTVRILDAKYLHLNKSTQSAMESSLQLMALLRSCSAFEPYRRTATG
                     ELELENVAEYLLLNHEFPRAVFFCIHRCQQTLDVIGEDPVSTVKSDSPRRLLGRLKAD
                     LEYLDIHDVLGENMDPFLARLLLRLNSVGDEISRTYFNTSIILPDERPRQQQQQQQ"
     gene            836..1543
                     /locus_tag="PKI33_14560"
     CDS             836..1543
                     /locus_tag="PKI33_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNM38289.1"
                     /translation="MRLRIEHTTTFTYDHPISEAYTEMRLKPLDADGQHCVSFQLTTE
                     PKDIVLRYTDHFGNDVRHFDVIQPHQKLMASAISEVITPDVYQSGSSNLSPLDEFNYL
                     SPTHYAPHSPDLGLFAASLKVSDSALENSLKVMHALFTTLTYEKGATDVNTTADKALS
                     LGRGVCQDFSHIMLAVCRLQGIPARYVSGYLYNNGHTAASHAWVDVLTSERGWVSLDP
                     THDREQTEQYVLHRFYW"
BASE COUNT          392 a          452 c          347 g          367 t
ORIGIN      
        1 agccttgttc gcagagacgt ataaagaggt caatgcgcag tcggtcatcc agttcatgtt
       61 ctgggagccg ttgaacggca atagtgtaac gagttgcatc accaaggcac gcgagaacgc
      121 ccgcagtgtg cgcgaacaga tcagcagtga aatgtgggaa gccatcaatc gcctgtatca
      181 tctcacgcgc aaccccgagc gcgccaaaga gagcaatccc cttgaattct tcaaggtcat
      241 tcgagaccgt gcccaatcct atcaaggcgt catttcagca accatgacgc acggcgaacc
      301 gtatcaattc atacagttgg ggttacacct cgaacgcgcc gataaaaccg tccgcattct
      361 ggacgcaaag tatctgcact taaacaaatc cactcaaagt gctatggaat cctctctgca
      421 actaatggca ctcctgcgtt catgcagtgc ctttgagccc tatcgccgaa cagccaccgg
      481 cgaattggag ctggaaaatg tggcagaata tctgttgctc aaccatgaat ttccgcgcgc
      541 ggtcttcttc tgcattcatc gctgccagca aacgttggat gtcatcggcg aagatccggt
      601 cagcacggtc aagagcgact cgccgcgccg cttgttgggc aggctaaaag ccgacctcga
      661 gtatctggac attcacgatg tcctcggtga aaatatggac ccattccttg cccgcctgct
      721 cttgcgcttg aactctgtgg gagatgaaat ttcccgcacc tacttcaata ccagcatcat
      781 cctgcccgat gaacgcccgc gtcaacagca gcaacagcaa caataaactc aactcatgcg
      841 attaagaatc gaacacacca ccacattcac ctacgatcat ccgatcagtg aggcgtatac
      901 cgaaatgcgt ctcaagcctc tcgatgccga cggtcaacac tgcgtctcgt tccaattgac
      961 caccgagccg aaagatatcg tcttgcgcta caccgatcat tttggaaatg acgtccgcca
     1021 ttttgatgtg atccaaccgc atcaaaaatt gatggcatct gctattagtg aagtcatcac
     1081 gccggatgtc taccagtcgg gctcttccaa cctttctcct ttggatgagt tcaattatct
     1141 ttcccccact cattacgcgc ctcattcccc agacctgggc ttgttcgctg cttctctcaa
     1201 agtttcagac tccgccttgg aaaattcgct caaagtgatg cacgccctgt ttacaacgct
     1261 cacctatgaa aaaggcgcca cagacgtcaa tactacagca gataaagcat tatctttggg
     1321 tcgcggagtt tgtcaggact tttcacacat catgctcgcc gtctgccgtt tgcaaggcat
     1381 ccctgcccgc tatgtgagcg gttacttata caacaacggt cataccgcag catcgcacgc
     1441 ctgggttgat gttctaacct ctgaacgggg ctgggtttct ctcgacccaa cgcatgaccg
     1501 cgaacaaacc gagcaatacg ttttgcatag attttattgg tgaatccgaa acgattcg
//
LOCUS       DAOREF010000815         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_74352_length_1555_cov_2.650000, whole genome shotgun sequence.
ACCESSION   DAOREF010000815 DAOREF010000000
VERSION     DAOREF010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74352_length_1555_cov_2.650000"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKI33_14565"
     CDS             <1..657
                     /locus_tag="PKI33_14565"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNM38290.1"
                     /translation="ALALESFLNVIRSQARQPVLRTLQRGIETANLHVYQKAHQMDAG
                     RMGTTLTAAYILGDMLYLAHVGDSRAYLIRDGRATCLTADHTTVGDMVRAKLIPAAKI
                     RTHAQRSILTKSIGTDLFVQPDILQQKLKPGDCLILCSDGLWSVVEDDDFARVASQSS
                     SVSEVSQNLVDLALAHHTDDNVSVVTFSVRSFMPVPAEQEPLPEESLGWFKKLRKMVS
                     "
     gene            672..>1555
                     /locus_tag="PKI33_14570"
     CDS             672..>1555
                     /locus_tag="PKI33_14570"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM38291.1"
                     /translation="MTNTMLQIGDQFDHYQIQAHMAQGGMSDVYRAFDLMHHREVVIK
                     VPDHSMIGDPAQFERFQRELEVMKSLSHPAILKGLDSGKHNRVPYLVTEFVQGESLRT
                     LMEKSGSLPTDQVVSLIQKLANGMAYCHKNEVIHRDLKPENILITSDGQPVIMDFGLA
                     LTKDSHRVTYSNLSATMGTPAYMSPEQIEGQRGDQRSDIYALGTIMYEMLAGHTPFTG
                     DTPMAVMAQHLNNNAPRLDKVNPSISVHLAAIVARCLARNPEDRYPDMDALIAVLDTP
                     GTVDISILDKSSAPPKSS"
BASE COUNT          421 a          417 c          333 g          384 t
ORIGIN      
        1 gccttggcgc tcgagtcatt tttgaatgta atccgttctc aagctaggca gcctgtttta
       61 cgcaccctgc aacgcggaat tgaaacagcc aatctgcatg tttatcaaaa agcccatcaa
      121 atggatgccg gacgcatggg caccacactc acagccgcat acattctggg agatatgctt
      181 tatcttgcac atgttggtga tagccgcgcc tatctcatcc gtgatggtcg cgccacttgt
      241 ttaacggcag accacaccac ggtgggagat atggtccgcg caaaactaat tccagccgcc
      301 aagatcagaa cccatgctca gcgctccatc ctcaccaaat ccataggaac cgatttattc
      361 gtacagcctg atatcctcca gcaaaagctc aaaccaggcg actgcctcat cctatgctca
      421 gatggattat ggtcagttgt ggaagatgat gatttcgcaa gagtcgcatc ccaatcctca
      481 tctgtttctg aggtgagcca aaatctagtc gatctagcac tggcgcatca caccgatgac
      541 aatgtatctg tggttacctt ttcagttcgc agctttatgc ctgttcctgc cgagcaagaa
      601 cctttacctg aagaatcact tgggtggttt aagaagctaa ggaaaatggt gtcatgaatg
      661 cctatgctat aatgacgaac actatgcttc aaatcggcga tcaatttgat cattatcaaa
      721 ttcaagcaca catggctcag ggcggaatga gtgatgtata tcgcgccttt gatcttatgc
      781 accatcgcga ggttgttatt aaagttcccg atcattccat gatcggcgac cctgcccaat
      841 ttgaacgctt ccaacgtgag cttgaagtta tgaagtcact tagccaccca gcgattctaa
      901 aagggctgga ttctgggaag cacaaccgcg tcccatatct tgtgacagaa ttcgtccaag
      961 gcgaatcatt gcgcacgttg atggaaaaaa gcggctcttt accgaccgat caagttgttt
     1021 ctctcatcca aaaattagcc aacgggatgg catactgcca caagaacgaa gtcattcatc
     1081 gtgacctgaa acctgaaaat atccttatca ctagtgatgg acaacccgtc atcatggatt
     1141 ttggtctcgc gttgacaaaa gactcgcacc gtgtcaccta ctccaaccta agcgccacca
     1201 tgggcacccc agcctatatg tcgcccgaac aaatcgaagg acaacgaggt gatcaacgca
     1261 gtgacattta tgccctaggt acgatcatgt atgaaatgct tgccgggcac actcccttca
     1321 caggcgatac ccccatggca gtgatggcac aacacctgaa caataatgcg cctcgtttgg
     1381 ataaagtaaa tccttccatt tcagtacacc ttgccgccat cgttgctaga tgtttggcgc
     1441 gcaaccctga agaccgctac ccagacatgg atgcgttaat tgcagttctc gataccccag
     1501 gaactgttga catttctatt ctcgataaat ctagcgcacc acccaaaagc tcaag
//
LOCUS       DAOREF010000816         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_74421_length_1554_cov_4.848566, whole genome shotgun sequence.
ACCESSION   DAOREF010000816 DAOREF010000000
VERSION     DAOREF010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74421_length_1554_cov_4.848566"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKI33_14575"
     CDS             <1..249
                     /locus_tag="PKI33_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HNM38292.1"
                     /translation="MGIGVMFSSIIIFFYQGGISLLAERLSTIISDPMMNEMTATGGV
                     LLMGVGISNLLELKKIRVGNFLPALVVAPLIVWVLGKL"
     gene            complement(285..359)
                     /locus_tag="PKI33_14580"
     tRNA            complement(285..359)
                     /locus_tag="PKI33_14580"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Lys,seq:ctt)
     gene            474..1196
                     /locus_tag="PKI33_14585"
     CDS             474..1196
                     /locus_tag="PKI33_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38293.1"
                     /translation="MQIAIIFFGVLILLVLIWLLLTELRREKSEEQSEPVSKNSQRVF
                     TKWLENFKTWSGERYPINIERLGFTVLWSIFFLSLLDHLQTRSGLTGTLIYFLVILPH
                     EAGHFICSPFGWFLSIAGGSIWQVLLFVLFGFIVLFRKRRNEALGWWAVAGHSLISLS
                     TYIADASARSLPLIPIPDPSRHDWGNMLEYLGLLQYDWLFAKISIITGGAIVLSCVVA
                     GLFFTWYQPQNLFNKPEIDTAK"
     gene            complement(1240..>1554)
                     /locus_tag="PKI33_14590"
     CDS             complement(1240..>1554)
                     /locus_tag="PKI33_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNM38294.1"
                     /translation="AMTVFMLSLIGFPPTLGMVGKFYLFRSALAGGFPGLALIGVVTS
                     LISAYYYLRVVVNMYMKEGDPEVEREPWLGLTTALTAVVTVAVSLIPQFLFTLAYTAV
                     MR"
BASE COUNT          391 a          340 c          381 g          442 t
ORIGIN      
        1 atgggaatcg gtgtgatgtt ttcttcgatc attatcttct tttatcaagg tggcatcagt
       61 ttgctggcag agagactttc cactattatc agtgacccca tgatgaatga aatgaccgcc
      121 acaggtgggg tattgttgat gggggtggga atcagtaatc tactggaact caaaaagatt
      181 cgggttggga attttctacc ggcgctggtc gtcgctccac tcattgtgtg ggtgttagga
      241 aagctataaa caaaaaacat ccccaaatgg ggatgttttt tgactggtga ctccgggggg
      301 atttgaaccc tcaaccaatt gattaagagt caactgctct gccattgagc tacggagcca
      361 tttcgtgcgg gcggtattat accgcaaagg ggtattgtgt caataattac aactcgtacg
      421 ttttttgcaa ggagagccgc cggaaaatag cttttataat caagaaggct tccatgcaaa
      481 ttgcgatcat ttttttcggc gttcttatcc ttctggttct aatttggctg cttttaaccg
      541 aattacgaag agaaaaatcg gaagagcaaa gtgaacctgt ttctaaaaac agccagagag
      601 tctttacaaa gtggcttgaa aacttcaaaa catggtctgg agaacgttac cccatcaaca
      661 tagagcgact tgggtttacc gtactctgga gcattttctt cctttcttta ttggatcatc
      721 tgcaaactcg ctcaggcttg accggaacgt tgatctattt tctggtaatt ttgcctcacg
      781 aagctgggca tttcatctgc tcccccttcg ggtggtttct aagcattgca ggcggatcaa
      841 tctggcaggt gcttttattc gtactctttg gcttcatcgt cctctttcgc aaacggcgca
      901 atgaagcttt gggttggtgg gcagttgcag gtcatagcct gatcagcctt tccacttaca
      961 ttgcagatgc cagtgcccgc agcctgcccc tcatccccat cccagacccg tcacggcatg
     1021 attggggtaa tatgctcgaa tatttggggt tgctacaata cgactggtta tttgcgaaga
     1081 tttcgattat caccggtggt gcaattgttc tttcttgtgt tgtagcaggg cttttcttta
     1141 cttggtatca gcctcaaaac ttattcaaca agccagaaat cgatactgca aaataaaaga
     1201 gcgctgacat ctgtcagcgc tctttttgtc tttttatgtt tatcgcatca ctgctgtata
     1261 tgccagtgtg aaaaggaatt gcggtataag gctcactgct acagttacga ctgctgtaag
     1321 ggctgtggtt aagccaagcc aaggttcacg ttcaacctca gggtcgcctt ccttcatgta
     1381 catgttgacc acaacgcgca agtagtagta tgcggaaata agagaggtca ccacgccgat
     1441 aagggcaaga ccggggaaac cacctgcgag ggcagagcgg aagaggtaga acttacccac
     1501 cataccaagg gttggaggga agccgatcag ggagagcatg aagacagtca tagc
//
LOCUS       DAOREF010000817         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_75121_length_1547_cov_5.040885, whole genome shotgun sequence.
ACCESSION   DAOREF010000817 DAOREF010000000
VERSION     DAOREF010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75121_length_1547_cov_5.040885"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PKI33_14595"
     CDS             <1..579
                     /locus_tag="PKI33_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38295.1"
                     /translation="ENEIGAYAKSKTLAERAAWDFINGPENSNHMELVAINPPLILGP
                     VPNKNFRTSVEVIRTYMLGQVPGVGRIKMGLVDVRDVAAAIILAMATPEAAGQRFLCS
                     GGMAWLKEIAGVLHAEFSPRGYKIPTLEFPSWAVRFLGLFDKKIARVTEELDRDTEQS
                     SEKAKRILKWSPRPLKEAIIAMGNSLIEHGFV"
     gene            644..>1547
                     /locus_tag="PKI33_14600"
     CDS             644..>1547
                     /locus_tag="PKI33_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNM38296.1"
                     /translation="MNYLPSTSRYQSMHYRRSGRSGLKLPAVSLGLWHNFGGVDVYEN
                     SRAMVLRAFDLGITHFDLANNYGPPPGSAEETFGQIMAKDLHPWRDELFISSKAGYYM
                     WEGPYGEWGSRKYLVSSLDQSLKRMGLEYVDLFYHHRPDPDTPLEETMQALDFIVRSG
                     RALYVGISNYPADKAREAAQILRSLGTPCLIHQPKYSMFERWVEDGLLQTLKEEGVGC
                     IAFSPLAQGLLTDKYLGGIPEGSRASKAHGFLKPAHITDERIAKVQKLNALAQQRGQT
                     LAQMALAWVLRHEGMTSVLIGASKV"
BASE COUNT          385 a          408 c          404 g          350 t
ORIGIN      
        1 gagaacgaga tcggcgcgta tgcaaaaagc aagaccctcg ccgaacgcgc cgcatgggat
       61 tttatcaacg gacctgagaa cagcaatcac atggaattgg tcgccatcaa cccgccgcta
      121 atccttggac cggtgccgaa taaaaatttc cgcacgtccg tagaagtgat ccgcacctac
      181 atgctggggc aagtccctgg tgtggggcgc atcaaaatgg ggttggtaga tgtgcgcgat
      241 gtcgcggctg ctattatcct agccatggcc acgcctgaag ccgcagggca gcgtttctta
      301 tgttcaggcg gcatggcatg gctaaaagag attgcaggtg ttttgcatgc tgaattctcg
      361 ccgcgcggat ataaaatccc tacccttgaa tttccgagtt gggctgtgcg ctttttaggc
      421 ttgttcgata aaaagatcgc gcgcgtcacc gaagaattag accgcgatac agaacaatcg
      481 agtgaaaaag cgaaacgcat tctcaaatgg agtccgcgcc cgttgaaaga ggcaattatc
      541 gctatgggaa atagcttgat cgaacacggg tttgtataaa attttaagat gaggtgacag
      601 tcactttaaa agtgactgtc acctttatat aataggagcc tttatgaatt acctcccttc
      661 cacctctcgt tatcaatcca tgcactaccg ccgctcaggg cgtagcggac taaaactccc
      721 ggccgtttcg cttggtttgt ggcataactt cggcggcgtg gacgtgtacg aaaacagccg
      781 cgccatggtg ctgcgcgcct tcgacctcgg tatcactcac tttgaccttg ccaataatta
      841 tggtccgcct cccggctcgg cagaagagac ctttgggcag atcatggcaa aagacctgca
      901 tccctggcgc gatgaattat tcatctcatc gaaagcaggg tattacatgt gggaaggtcc
      961 gtatggagaa tggggttcgc gcaagtatct cgtctccagc ctcgaccaaa gcctcaagcg
     1021 catgggcttg gaatacgtgg acttgttcta tcatcaccgc cccgaccctg atactccgct
     1081 cgaagaaacg atgcaagcgc tcgatttcat cgtccgcagt ggacgggcat tgtacgtggg
     1141 catttcaaat taccccgctg ataaagcgcg tgaagcagct caaattctac gatcactggg
     1201 cacgccttgt ctgatccatc agccgaagta cagcatgttc gaacgctggg tggaagatgg
     1261 tctgttacag acgttgaaag aggagggagt tggctgtatc gccttctcac cgctcgctca
     1321 aggcttactc actgataaat atctcggcgg cattcctgaa ggatcacgtg cttcgaaggc
     1381 gcatggcttc ctcaaaccgg ctcacatcac cgatgaaaga atcgccaagg tgcaaaagct
     1441 caacgccctc gcccagcaac gcggacaaac actcgcacaa atggcgctgg cttgggtgct
     1501 acgccatgaa ggcatgacct ccgtgctaat cggtgcaagc aaagtca
//
LOCUS       DAOREF010000818         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_75220_length_1546_cov_3.820926, whole genome shotgun sequence.
ACCESSION   DAOREF010000818 DAOREF010000000
VERSION     DAOREF010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75220_length_1546_cov_3.820926"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKI33_14605"
     CDS             complement(<1..521)
                     /locus_tag="PKI33_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNM38297.1"
                     /translation="MDKKLSRRDFLRILKLFGLGAFFAYETRYVSAIEPSWLEVTQVA
                     LELQGLPKAFHNYRILQLSDIHMGGWMNRERFSAVLDVVEQQSFDLLVITGDFVIGGR
                     SWTENLDLAAIDFIELMKPVTKRRLTLGVMGNHEYKTDPVLARKMLAEAGVMELKNDV
                     HLLEKDGEALYIAG"
     gene            complement(583..>1546)
                     /locus_tag="PKI33_14610"
     CDS             complement(583..>1546)
                     /locus_tag="PKI33_14610"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNM38298.1"
                     /translation="TDDVDIAKKIAKAVRQSSGGLRYVKGLGLLVDGRAQVSMNLTNY
                     RETPIARVVEFIRREAERYGVGIHHSELVGLIPQEALVDAAVWYTQLDAFSPEQVLES
                     RLFSTPTASASNALKPASFIEELAAPTPTPGGGSAGAYAGAMGAALVAMVAGVTIGKK
                     KYAEVEAEMQAIRVVAENLRKELTQAVDDDASSFEVLMAKFKMPKETEEQKSAREAAI
                     TQATLNAAHIPLHVSEHAVKVMELALKCAKSGLQSAISDAMSGFAMARASLTAAGYNV
                     RININSLEDKSLGDRMLSELAELEKAADKLEGEIRTVMKERGGI"
BASE COUNT          401 a          428 c          350 g          367 t
ORIGIN      
        1 ccagcaatat acaaagcctc tccgtctttt tccaaaagat gaacgtcatt cttaagctcc
       61 atcacccctg cctcagcaag cattttccga gcaaggactg gatccgtctt atattcatga
      121 tttcccatca cacccaaggt aaggcggcgc tttgtaacag gcttcataag ctcaataaaa
      181 tctatagcag ctagatccag gttttccgtc caagaacgtc cgccgattac aaaatctccc
      241 gtgatcacca gtaagtcaaa actttgttgc tccaccacat ccaaaacagc ggaaaagcgt
      301 tctcgattca tccaaccacc catatgaata tcgctcaatt gaagaatacg ataattatga
      361 aaagcctttg gtaacccctg tagctccaaa gcaacctgtg tcacttcaag ccaggaaggt
      421 tcaatagcac ttacatatcg ggtttcatat gcaaaaaaag cacctagccc aaagagcttg
      481 agaatcctta gaaaatctct tcttgataat tttttatcca taactgcttc taaaatcatc
      541 tcaaacgaaa ctaaaaccca ccttaaaata aaaaggcggg atttagatcc cgcctcgttc
      601 tttcatcacg gttcgaatct caccttcgag cttatctgcc gccttttcca actctgccaa
      661 ctcgctcaac atacggtcgc ccaatgattt atcttcaagc gaattgatgt taatgcggac
      721 gttgtacccg gcagccgtca gggatgcgcg tgccattgca aagccagaca tggcatcact
      781 aatagcgctc tgcaaaccag acttggcgca tttaagagca agctccatga ccttgactgc
      841 atgttcgcta acgtgcagtg gaatatgggc ggcgttcaag gttgcctgcg taatagccgc
      901 ctcacgagcg gacttttgct cttcggtctc tttcggcatt ttgaattttg ccatgagcac
      961 ttcaaaggat gaagcatcat catcgacagc ttgggtaagc tctttacgaa gattctctgc
     1021 caccacacgg atggcttgca tttcagcttc gacctcggcg tatttcttct tgccaatggt
     1081 cacgcctgcc accatggcaa ccaaggcagc gcccatggca cccgcataag cccccgcgga
     1141 cccgccgccg ggcgtcggtg tcggcgcggc taattcttcg atgaaagaag caggcttgag
     1201 cgcgttggag gcggaagcgg tgggagtaga aaacagcctg gactcgagaa cctgttcggg
     1261 agagaaagca tccaactggg tgtaccagac cgcggcatcc acaagggctt cttgtggaat
     1321 caaaccaacc agttcgctgt gatgaatgcc gacgccataa cgttcggctt cgcggcgaat
     1381 gaattcgacc acgcgggcga tgggcgtttc gcggtagttg gtgaggttca tcgaaacctg
     1441 ggcgcggcca tccacgagca gaccgagacc cttgacgtaa cgcagaccgc ccgaagactg
     1501 acgcacagct ttggcgatct tcttggcgat gtccacatcg tcggtg
//
LOCUS       DAOREF010000819         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_75500_length_1543_cov_5.004032, whole genome shotgun sequence.
ACCESSION   DAOREF010000819 DAOREF010000000
VERSION     DAOREF010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75500_length_1543_cov_5.004032"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..626)
                     /locus_tag="PKI33_14615"
     CDS             complement(84..626)
                     /locus_tag="PKI33_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38299.1"
                     /translation="MSEFTMKLIKCPSCGGPIDPPSGESTAKCPYCGSAVVIPESLRK
                     PAVSNQNQSSLTSSANVFGVDLNSMMGYGAQWSQVVQLAQAGQKEEAVKKYMSFSSIS
                     EMDARRTVEGLANTQVYDVSAYTTSVANAYAPMVQSYTDTAKKITAWSFGFSCLITGF
                     IMLTVLLILGATFWAVFKSF"
     gene            625..>1543
                     /locus_tag="PKI33_14620"
     CDS             625..>1543
                     /locus_tag="PKI33_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNM38300.1"
                     /translation="MALLLWFAMVKLYEHQIFSIGAIRTSQLLALSAGWLGVRIASFQ
                     LDEEMFMTEQFTFGGEIVWRPSQAQVDGANLTAFMQMWKLSDFDALMRKSTHDVAWFT
                     EAVLKFLDIRFYEPYSQVIDLKNGIQFPKWCVDGKMNIVHNCVKKWVENEADRKKIAI
                     AFEGEDGITKTLTYEDVDKEINRTANALRTLGLGKGDAIGIFMPMTPEIVIALLAIAK
                     IGGIILPLFSGYGAGAIASRMVDADAKALFAADGAFRRGKAVEMKSVADEAAEQIPTL
                     KHMIVLKRTGQDVKMKAGRDHWWHELVDVQ"
BASE COUNT          414 a          308 c          406 g          415 t
ORIGIN      
        1 gcaaaagacc ggctttttgt aagaattaag ttgtaacaag aaagaagcgg atggttgaaa
       61 ttccatccgc ttcttttata aggttagaaa gatttgaata ctgcccaaaa tgtagcccca
      121 aggatcagta gaacggtcag catgataaac cctgtgatca aacagctaaa accaaacgac
      181 caagcggtga tctttttggc ggtgtcagta tagctttgaa ccataggagc ataggcattg
      241 gcaacgctcg tggtataggc gctaacatca tatacctgtg tatttgccag cccttccaca
      301 gtgcggcgag catccatttc actgatgctg gagaagctca tatatttttt aacggcttct
      361 tctttttgtc cggcttgagc aagctggaca acttgagacc actgcgcccc atagcccatc
      421 atggaattta agtccacgcc gaacacattt gcagatgacg tgagagacga ttggttttgg
      481 tttgagactg ctggttttct caaactttct gggatgacaa cggcactgcc gcagtagggg
      541 catttggcgg tgctttctcc gctgggcggg tcaatgggac ctccgcaact ggggcactta
      601 attagcttca tcgtaaattc agacatggca cttctcctct ggtttgcaat ggttaaatta
      661 tatgaacacc agatttttag cataggtgcc attcgtacta gtcaacttct tgcattgtcc
      721 gctggctggc ttggggttag aatcgcttca ttccaacttg acgaggagat gttcatgacc
      781 gaacagttta cgtttggcgg tgagatcgtt tggcgacctt cccaagcgca ggtggatggg
      841 gcgaatttaa cagcattcat gcagatgtgg aaactctctg attttgatgc gctaatgcga
      901 aagtcaacac atgatgttgc ctggttcact gaggcggtct tgaaatttct tgatattcgg
      961 ttttatgaac cgtattcaca agtgattgac ctaaagaatg gaattcagtt cccaaagtgg
     1021 tgtgtagatg ggaagatgaa cattgtgcat aattgcgtta aaaaatgggt ggagaatgaa
     1081 gctgaccgta aaaagatagc aatcgcattt gaaggggaag atggaattac aaaaacactc
     1141 acctacgaag atgtagataa agagatcaat aggactgcca atgctttgcg aacattagga
     1201 ttgggcaaag gggacgctat tggtatcttc atgccaatga cgccagagat cgttattgcc
     1261 ttattagcaa ttgcgaagat cggcggaatc attctgcctt tgttttctgg ttatggtgcg
     1321 ggcgctattg cctcccgtat ggtagatgcg gatgcaaagg cactatttgc agcggatggc
     1381 gccttccgac ggggcaaggc tgtggaaatg aagtcggtgg ctgatgaagc ggcggagcaa
     1441 atcccgaccc ttaagcacat gatcgtcttg aagcggactg gtcaagatgt gaagatgaaa
     1501 gcaggaagag atcattggtg gcatgagttg gtcgatgtgc aaa
//
LOCUS       DAOREF010000820         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_75801_length_1540_cov_4.264646, whole genome shotgun sequence.
ACCESSION   DAOREF010000820 DAOREF010000000
VERSION     DAOREF010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75801_length_1540_cov_4.264646"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1540
                     /locus_tag="PKI33_14625"
     CDS             <1..>1540
                     /locus_tag="PKI33_14625"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNM38301.1"
                     /translation="IRAFSHWKTPGTRTLGALILTMAVWAAFYILEVIHPSLEVKILA
                     RKMLYFGMTMAPPFWLAFALRYTGLGAWWSQQGRVFLFAIPGGIAFLFGLTNEFHHLI
                     WSDLSLSATKPVLLTIEYGIGFWVYAIVAYLQIFVGLAIYLIAHFKNKKAFRTKTGIV
                     LAGLLLTAIVNVLFLLFGNSLNVDPTPLSFALSAPLIAWGFFRFGVSSLFPLAASLVV
                     ENLQDAIIIVNREDEITDINQTAAELLDLKTVKEKSNIFTLLPFAEQLKPIWDAQKSS
                     IKLETNFRGASQWVEAQVIPIKDHEDSLLGRVIVLHDITKEQALLRAERRRSQQLSLL
                     EQTGRYIAGSFDEQEILQRTVDAITQQFGYPETAISLLKNNMFEIAAIFGTEDFGYRQ
                     GYRQEIGSGIIGYTASIKKTYVSRNVSQDPYYYSTSTKSGSAICTPIFKQDALYGVLY
                     VESFDLDAFDELDIITLETLASQMSASLQRAELYAQTQNDLLTLRTIQNISKLITSSL
                     DLETI"
BASE COUNT          432 a          348 c          315 g          445 t
ORIGIN      
        1 gattcgcgcc tttagccatt ggaaaacgcc cggaacccga acccttgggg cactgatatt
       61 gacaatggca gtttgggcgg cattttatat tcttgaagtt atacatcctt ctcttgaagt
      121 aaaaattcta gcgcggaaaa tgctttactt cggtatgacc atggcaccgc cattttggtt
      181 ggcattcgca ttgcgataca cagggcttgg agcgtggtgg tcacaacaag ggcgtgtctt
      241 tttattcgcc attccgggtg gaattgcatt cctgtttggc ttaactaacg aatttcatca
      301 tttgatttgg tcagaccttt ctttatccgc aaccaaaccg gttctgctca ctattgagta
      361 tggcataggg ttttgggttt acgcaattgt tgcctacctt cagattttcg tgggtcttgc
      421 gatttatctt atagcacatt tcaaaaacaa aaaagccttt cgtacaaaaa caggtatcgt
      481 tttagctggt ttacttctga cagccatagt caatgtcctt tttcttcttt ttggaaatag
      541 cttaaacgta gacccaactc cgctcagttt tgcattatct gcaccattga ttgcatgggg
      601 ttttttccgt tttggagttt caagtctatt tcccctcgcg gcatcgttgg tggtagaaaa
      661 ccttcaagat gcaatcatca tcgtaaaccg tgaagatgag attactgaca ttaaccaaac
      721 agcagctgaa ttactagact taaagaccgt aaaagaaaaa tcaaatattt tcacgcttct
      781 gccttttgca gagcaactta aaccgatatg ggatgcgcaa aaaagctcga ttaagttgga
      841 aacgaatttt agaggtgcat cacaatgggt tgaagcccaa gtcatcccca ttaaagacca
      901 tgaagatagt ttactgggca gggtaattgt tctgcatgac attaccaagg agcaagctct
      961 gttgcgtgca gagagacgtc gctcccaaca gttatcccta ctggagcaga ccggcaggta
     1021 tattgctggt agcttcgacg agcaagaaat ccttcaacga actgtggatg ccattacaca
     1081 acaatttggc tacccagaaa ctgccatttc tttattgaaa aacaatatgt ttgaaatcgc
     1141 cgcaatcttt ggcacagaag atttcggcta ccgtcaggga tacagacaag aaattggctc
     1201 aggcatcatt gggtataccg ccagcattaa gaaaacttat gtatctcgga acgtctcgca
     1261 agacccctat tattattcaa caagtacaaa gtctggatcg gcaatctgta ccccgatctt
     1321 caaacaagat gctctctacg gcgttctata tgtagaaagt tttgacctag atgctttcga
     1381 tgagctggat attattaccc ttgaaaccct tgcaagtcag atgtccgcat cactacaacg
     1441 agccgaactg tatgcacaaa cccaaaatga cctacttact ctgcgaacga tccaaaacat
     1501 ttcaaagctc attacaagtt ctctcgacct tgagaccatc
//
LOCUS       DAOREF010000821         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_75969_length_1538_cov_5.002023, whole genome shotgun sequence.
ACCESSION   DAOREF010000821 DAOREF010000000
VERSION     DAOREF010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75969_length_1538_cov_5.002023"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKI33_14630"
     CDS             <1..427
                     /locus_tag="PKI33_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNM38302.1"
                     /translation="ILGVTILLSIFTGAIKSYRAVFVQSKVSPYIEAARAYGTTDRRI
                     IFSYLIPRMIPLLIPALVSSVPSYVFLEASLAVLGLGDPVLPTWGKIIENAFAEGALY
                     RGLYYWVLEPAALLMLTGLGFAMLGFALDRIFNPRLRDV"
     gene            428..1390
                     /locus_tag="PKI33_14635"
     CDS             428..1390
                     /locus_tag="PKI33_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38303.1"
                     /translation="MANNDNLLRLEDLVLHFGTTRGSVQAVDKVNFSLNANQAVVVLG
                     ESGCGKSSLAKAILRLLPRNVVSYSGRVYLNGNDVMQLDDEEFRQNIRWASISMVPQA
                     AMNSLNPVIRVGDQVAEPAIIHLGVSKQEAVSLVKKMFQHVGVPEDFITRYPFELSGG
                     MRQRVAIAMALVTSPSLILLDEPTSALDVLTQASIFNVLKRLKGELGISFILITHDIA
                     TSSELADSVAVMYAGQIVETSDAHRFFAEPLHPYSRMLMASVPRLRANTEPEFITGQP
                     PSLLEPPKGCRFADRCPKRFDKCSEEPPVVNVDGIQVKCWLYTS"
BASE COUNT          351 a          342 c          371 g          474 t
ORIGIN      
        1 cattctcggt gtgactatct tgcttagtat cttcactggt gcaatcaaat cataccgtgc
       61 tgtgttcgta caatccaaag tttcacccta tatcgaggct gcacgtgcat acggcacgac
      121 agatagacgc atcattttca gctatctcat ccctcgcatg atccccttgc taattcctgc
      181 cttggtttcc tctgtacctt cttacgtatt tttggaagca tccttggctg ttcttggttt
      241 gggtgaccct gttttgccca catggggcaa gattattgaa aacgcctttg cagaaggcgc
      301 cctctatcgt ggtttgtatt attgggtgtt agagcctgct gctttgttga tgttaaccgg
      361 gttgggtttt gctatgttag gctttgcact tgaccgtatt ttcaatccaa gattgaggga
      421 tgtataaatg gcgaacaacg ataaccttct tcgacttgag gatttggttt tacattttgg
      481 cacgacccgc ggctctgttc aagctgtgga taaagtaaat ttttctctga atgctaatca
      541 ggcagttgtt gttttaggag agtcgggctg cggaaaaagc tcgctcgcca aggctattct
      601 tcgccttctt cctcgtaatg ttgtttctta ttctggacgc gtttacttga atggaaacga
      661 tgtgatgcag ttggacgacg aagagtttcg ccaaaacatc cgttgggcat ctatttccat
      721 ggtcccgcaa gcggcgatga actctttgaa ccctgttatt cgtgtgggcg atcaggttgc
      781 agaaccagct atcatccact taggagttag caagcaggaa gctgtttcgc tggtaaagaa
      841 aatgttccag catgtgggcg tgcccgaaga ctttatcacc cgctatccct ttgaattgag
      901 cggtggtatg cgtcaacgtg ttgccattgc gatggcgctt gttacttctc caagcttgat
      961 cctattggat gaacccacct ccgcgttgga tgtattgacc caagccagca tctttaatgt
     1021 gctaaaacgc ctcaaaggcg aactgggtat tagctttatt cttattacgc acgatattgc
     1081 tacatcgagt gaacttgccg atagtgttgc ggtgatgtat gcgggtcaaa ttgtagaaac
     1141 gagcgatgct catcgctttt tcgcggaacc gcttcacccg tattctcgca tgttgatggc
     1201 aagtgttcca cgcttacgtg ccaatacaga accagagttt attaccggac aaccccccag
     1261 cttgttggaa cctcccaaag gttgtcgttt tgcggatcgt tgccctaaac gttttgacaa
     1321 gtgttctgaa gagccgcctg ttgtaaatgt agatggcatt caggtcaaat gttggttata
     1381 cacatcctag tttgtgtaag ataaaatcta aaaagaggtc tacaagacat gacagataca
     1441 caaagagatg tgcttctctc gataaatgac ctgcacgtgt ggttcgaatt acgtcgcttg
     1501 ggttttggac acgccggtta tgtgaaggca gttgatgg
//
LOCUS       DAOREF010000822         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_76004_length_1538_cov_2.424815, whole genome shotgun sequence.
ACCESSION   DAOREF010000822 DAOREF010000000
VERSION     DAOREF010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76004_length_1538_cov_2.424815"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKI33_14640"
     CDS             <1..1066
                     /locus_tag="PKI33_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38304.1"
                     /translation="TAYLFFSLLALLAFDKTDGKSFIWLILAAVLAFYTQQVRTIGIA
                     LTVSLLVILVLSKRYKEMGLVAVLFIVGFTLQSWLNLRNGGTVISSGYEAQVLSGSVV
                     EKVGQVWSNASGYLDQVLAGSLVPVFGTRLDTLFSWLFILLNIAILFLIVSGMLTTKL
                     KFEWMHIYFVIYTAGILAFWNPQVGSVKARFLIPILPFLYFYFLSGLKWITRGNSRTV
                     IGTASVITLILLARNVQDWRAPVREQMTDLSIGTSWVAENAPADAIVMVNEPVPAYVH
                     VQRKTINFPKNGQDLEQYVENQGIDYIVIAPLLQSPISRELSKDIQEIQFQIESQPNT
                     FQVVFESKENNVTVYQHIGK"
     gene            complement(1123..>1538)
                     /locus_tag="PKI33_14645"
     CDS             complement(1123..>1538)
                     /locus_tag="PKI33_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38305.1"
                     /translation="GNVAANLANNAVDYAVRSNVPSRLVYQVKTHAEGNVLGWIVPSR
                     GTGWFDFLPYSTRIQILNLPFAARAYAPSYEFKIGNLYGPTVLKLQKQRDLLVDKYTL
                     EKVGEMTDQQERWAIPALTLVAMFERTRVKQMADW"
BASE COUNT          417 a          353 c          354 g          414 t
ORIGIN      
        1 gacagcatat ttatttttct ctctgttggc attgctcgct tttgacaaaa cagatgggaa
       61 gagtttcatt tggctcatcc ttgccgcagt cctcgcgttt tacacccaac aagtccgcac
      121 aattgggata gcactcaccg tctcgttgct tgtcatctta gtgctgtcca aacgctacaa
      181 agaaatggga ctggttgccg tgctctttat agttggattc actctccaga gttggctcaa
      241 cctccgcaac ggtgggacgg tcatctcatc aggctatgaa gcgcaggtct tgagtggttc
      301 ggtggtcgag aaggttgggc aggtctggtc aaatgcatcc ggctatttgg atcaagtcct
      361 tgccgggtca ttagttccgg tcttcggcac acggctggat acgctattca gttggctgtt
      421 cattctttta aatatcgcaa tcttgttctt gattgtgtcg ggcatgctca ccaccaagct
      481 caagttcgag tggatgcata tttattttgt catttacacg gctggtattt tggcgttttg
      541 gaatccgcag gtgggaagtg taaaggcacg cttcttgatt cccattttgc catttcttta
      601 cttttacttt ttgagcggat tgaaatggat cacaagaggc aactcgcgta ccgttattgg
      661 caccgcatct gtaattacac ttattttgct agcccgcaat gtacaagact ggcgcgcccc
      721 cgtccgtgag cagatgacag acctttccat tggaaccagt tgggttgctg aaaatgcgcc
      781 agccgatgcg atcgtaatgg tcaatgaacc cgttcctgcc tatgtacatg tacagcgtaa
      841 gaccattaac ttcccaaaga acggacagga cttagaacaa tatgtagaga accaaggtat
      901 tgattacatc gtcattgccc cgctattgca atcgccaata agcagagaat taagtaaaga
      961 tatccaagaa atacaattcc aaatcgaaag ccaaccgaac acctttcaag tagtctttga
     1021 aagtaaagaa aacaacgtaa cggtatatca acacattgga aaataaaaat acttccggca
     1081 aaagaatgcc ggaagtattt aaacaataat taacttacta atttaccaat ctgccatctg
     1141 cttgacgcga gtgcgctcga acatggcaac caaggtcaac gcagggattg cccaacgttc
     1201 ctgttggtcg gtcatttcgc caactttttc aagggtgtat ttatcaacga gcaagtcacg
     1261 ttgcttctgt aatttcaaca cggtaggacc atacaagttg ccgatcttga attcataaga
     1321 aggggcatag gcgcgtgcag caaacgggag attgagaatc tgaatgcggg tggaataggg
     1381 taaaaagtca aaccagcccg tgccgcgcga agggacaatc caccccaaaa cattaccttc
     1441 agcatgggtt ttgacctgat acacaagccg agacgggaca tttgagcgaa ctgcataatc
     1501 gactgcattg ttggctaagt tggcagcaac attaccaa
//
LOCUS       DAOREF010000823         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_76452_length_1533_cov_2.937754, whole genome shotgun sequence.
ACCESSION   DAOREF010000823 DAOREF010000000
VERSION     DAOREF010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76452_length_1533_cov_2.937754"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..891
                     /locus_tag="PKI33_14650"
     CDS             334..891
                     /locus_tag="PKI33_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38306.1"
                     /translation="MKNTFAILFVFVLSACATVPISSSPEPPVSIETSPALTAEVPET
                     QVPAQSIYTSTKYGYSLSYPDTLNVIVVSDEYVEIGEKITVEVMNVDPTAPRGDGAVI
                     ETISDTQVSGYPAKLLTGYIGSVGGYIPQQFRKVVVERNGSYFVVTLYALGLHVTDGD
                     LSKIAELAMVDISLFDNVTASMQIP"
     gene            complement(1018..>1533)
                     /gene="serC"
                     /locus_tag="PKI33_14655"
     CDS             complement(1018..>1533)
                     /gene="serC"
                     /locus_tag="PKI33_14655"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNM38307.1"
                     /translation="IYAGAQKNLGTSGATLVIMKESLLGKVQRAIPTMLDYRSHIKDN
                     SLHNTPSTFAIYVCMLTLRWLKEQGGITAMEKRNRAKAALLYAEIDRNALFEGNVAVE
                     DRSLMNIPFRLPSEELEETFAKEAKKNNLIGLKGHRSVGGMRASVYNAVTVEGAKALV
                     DFMKEFQKKNG"
BASE COUNT          386 a          331 c          340 g          476 t
ORIGIN      
        1 cagctcccga ataggatccg tgacttggtg cttaatgacc aggaattgcg caatgaaatc
       61 tgttggttca tgtttagtca ttaggaaaac cttgtcccat ggggttcata tgggagttgc
      121 gaaatttcgg ttcgaaagtg ctcttgtttc gctgtcttag gatggataaa tatttgattt
      181 ccccctcaaa atggagcttt tacagtgaat tgcttctgaa tcgtatctgt ttcaatgtag
      241 ataatataga ccattggggg actttatcaa atccgtcatt caattagaat cgcgcaatgg
      301 tcaataattt tcaaatatta aaaaaaggaa cttatgaaaa atacgtttgc gatcttgttt
      361 gtattcgttc tatcagcctg tgctactgtg cccatttcct cctcgcctga accgccagtt
      421 tcaattgaaa cttctcctgc tcttactgct gaagtgcctg aaacccaggt tcctgcacaa
      481 agcatctaca ccagtacaaa atatggatat tctttgagtt atccagacac actgaatgtc
      541 atcgttgtaa gcgatgagta tgttgaaatt ggcgaaaaaa ttacagttga ggtaatgaat
      601 gttgacccga ctgcgcctcg cggagatgga gccgttattg aaaccatctc cgatacccaa
      661 gtttcgggtt atcctgctaa attattaacg gggtatatag gctctgttgg tggttatatc
      721 cctcaacagt ttcggaaggt tgtagttgaa cgaaatggca gctattttgt cgtcactctt
      781 tatgcgcttg gcttacatgt tacagacggc gatctttcga aaatcgcaga acttgcgatg
      841 gtagatattt ctttgttcga taatgtaacg gcatccatgc agatacccta gttccatctt
      901 cgaatgctcc actctcgctg gggaagagtt tttggcgtcg aaagccttgt gcaaaaattt
      961 aaaaaagcct ttgagtaaaa actcaaaggc tttttaattt attgtttcct cgttttatta
     1021 tccgtttttc ttttgaaact ctttcatgaa atcgaccaat gccttggcac cttcaacggt
     1081 tacagcattg taaacggatg cacgcatacc gccgacagac cgatgaccct tgaggccgat
     1141 caggttgttc ttcttggctt ccttggcaaa ggtctcttcg agttcttcac tgggcagacg
     1201 gaaggggata ttcatcagtg aacggtcttc gacggctaca tttccttcaa acaaagcatt
     1261 gcggtcaatt tccgcataca gtaacgcagc tttcgcgcgg ttacgttttt ccatcgcggt
     1321 aatgccgcct tgttccttca gccagcgaag cgtgagcatg cagacataaa ttgcgaaagt
     1381 actgggtgtg ttgtggagtg aattgtcttt gatatgcgaa cggtaatcca gcatcgttgg
     1441 aatggcacgc tgcactttcc ccagcaggct ttccttcatg atcaccagtg ttgcgccgga
     1501 agtgccgaga tttttttgcg caccggcata tat
//
LOCUS       DAOREF010000824         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_76643_length_1531_cov_4.889566, whole genome shotgun sequence.
ACCESSION   DAOREF010000824 DAOREF010000000
VERSION     DAOREF010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76643_length_1531_cov_4.889566"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKI33_14660"
     CDS             <1..429
                     /locus_tag="PKI33_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNM38308.1"
                     /translation="EDGLAQDDWDAWKSLVKTIGDKCQIVGDDLLVTNPERVRRAIKE
                     NAANALLVKVNQIGSLTETIEAVDLCQRAGWRAISSHRSGETEDATIADLAVALNAGQ
                     IKTGAPARSDRVAKYNQLLRIESELGDTAKYAGWDALKVK"
     gene            631..>1531
                     /locus_tag="PKI33_14665"
     CDS             631..>1531
                     /locus_tag="PKI33_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNM38309.1"
                     /translation="MKITADQAFLRETNLSLVVRLIHNQSPLSRAQLAGITGLNKSTV
                     SSLVDELIERGLVYETGSNTGGTGRPATLLEINPNAGHIIGVELGVDFVSVAVTDILG
                     NIVWRRREDANPADDQEKMINQTLQIVKDAMSIGKRKNYKFLGLGLSTPGTVDIQKGL
                     LIFAPNLHWHNVPFVQIFSEQTKLRVFVENDANAAAIGEHLFGTARHTNDFLFVFAGV
                     GIGGGLFLDGKLYRGKNGYAGEIGHSPIMAEPSQTVCHCGNRGCWETYANQYSIIQRV
                     QARLEVKRSSIIPKLMAEQNAPLG"
BASE COUNT          415 a          343 c          382 g          391 t
ORIGIN      
        1 gaagatggtc ttgctcaaga tgactgggat gcctggaaat ctctcgtcaa aaccattggc
       61 gataagtgcc aaatcgtggg tgatgatttg ttggtcacga accctgaacg tgtccgccgc
      121 gctattaagg aaaatgctgc gaatgcactc ttggtaaaag tgaaccagat cggttctctc
      181 actgagacca ttgaagctgt ggacctctgt cagcgtgctg gctggcgtgc catttcatct
      241 caccgctctg gcgagaccga agatgcgacc attgccgacc tcgcagttgc actcaatgct
      301 gggcaaatca agaccggcgc ccctgcccgt tctgaccgtg tggcgaagta caatcaactt
      361 ctgcgcatcg aatcagagct gggtgacact gccaagtatg caggctggga tgctctgaaa
      421 gtaaagtaag ctgaaaaact ccgttgagac aaactcaacg gagttttttt atagttgcat
      481 aaactcaagg ctaggaaaaa gctagaaagg ggtaaacagg cacaaggtcc agaaagaagc
      541 gaccttgcgc ctgtcgatgt ttaatttgac aatccccttc aaagcgctat aatttgttgg
      601 ttgccccaac atactaatcc tagggagcct atgaaaatca ctgccgatca agcattttta
      661 cgagaaacca atctttcatt ggtcgtaagg ttaattcata atcaatctcc attatcgcgc
      721 gcacaacttg ctggtattac cgggctgaac aaatcgaccg tttcaagcct ggtcgacgag
      781 ttaattgaac gcggcttagt gtatgaaacg ggcagcaata caggtgggac gggacgccca
      841 gctaccttgt tagagatcaa cccaaacgcg ggtcatatca ttggtgtaga actcggcgtg
      901 gactttgtgt ctgttgctgt gacagatatt cttggcaata tcgtttggcg caggcgtgaa
      961 gatgcaaacc cagcagatga ccaggaaaaa atgatcaatc aaacattgca gatcgtaaaa
     1021 gatgcaatgt ccattggcaa acgcaaaaac tataagtttt tagggctggg actttccaca
     1081 ccaggcacgg tagatattca aaaaggtctt ttgatttttg cgccaaatct tcattggcac
     1141 aatgtgccgt ttgtgcaaat tttttctgag caaactaagc taagggtttt tgttgagaac
     1201 gacgcaaatg cagccgccat tggcgaacat ctttttggta cggcacgtca taccaatgac
     1261 tttctttttg tgtttgcagg ggttggaatt ggcggcgggc tattcttaga tgggaaactg
     1321 tatcgcggca aaaatgggta tgcgggtgag attggtcact cccctatcat ggcagagcct
     1381 tcacaaactg tttgtcattg cggcaaccgt ggttgttggg aaacctatgc caatcaatat
     1441 tccattattc agcgggtgca agcccgtttg gaagttaaac gctccagcat catccccaaa
     1501 ttgatggcag agcagaatgc gccattaggt a
//
LOCUS       DAOREF010000825         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_76737_length_1530_cov_4.078644, whole genome shotgun sequence.
ACCESSION   DAOREF010000825 DAOREF010000000
VERSION     DAOREF010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76737_length_1530_cov_4.078644"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..>1530)
                     /locus_tag="PKI33_14670"
     CDS             complement(185..>1530)
                     /locus_tag="PKI33_14670"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNM38310.1"
                     /translation="QGQVYPHWAFELAKLYLNTEEPIFGNSSVKFNGQNIPLRSNELP
                     IYFAGPAGTYPTYSASDVHDGITLEQNPDAFRGKIVLIGATTITLQDIYPTPFSATDP
                     TPGVEVVANTVDTIINGKFITYAPPWLALLLIIVAAGMAYLITLSKRPSFTLTALGIS
                     ILLYLVAAYFVFSSQRYILPTIAPTLMLFLGVILPTLEQAVSQEQEKRRVRNLFSRFI
                     SPEMVDQMMKTQDINSLNKRADLSIIFSDIRGFTTLSEKMTPEGVVALLNPYLEAMSQ
                     VIYRHGGTVDKYEGDAIIAFFGEPVPYKDHAVRALRASMDMRKALAELRDKWAKEGRP
                     SQIEMGVGINSGEVFVGLLGSAQRINYTVIGDNANLASRLQDLTKNYAWPILISESTY
                     QQVKDEFDCELADAVTVKGKTKAVNVYKVIGEKGAPESEQLQAWTSDQVKPKHTS"
BASE COUNT          398 a          340 c          380 g          412 t
ORIGIN      
        1 gtagtatagc cataactata ccaaaaaaac gctttttgta caattatgta caagtctttc
       61 acaaataaac aggggattaa aaatccgcct ccagcgtgga tttctgcctg gttgccagac
      121 caagcctacg tctacgcctg agccggtaca aaaacggagc gagaattctc gctccgtttt
      181 tgcgtcatga tgtgtgtttg ggcttcacct ggtcgcttgt ccaggcttgt agttgttcgc
      241 tttctggagc gcctttttcg ccaatgacct tgtatacatt gaccgccttg gttttccctt
      301 tgacggtgac tgcatctgca agttcgcaat cgaattcatc cttgacttgt tgataggtgc
      361 tttcactgat gagaatgggc caggcgtagt ttttggtgag gtcttgcagg cgagaggcaa
      421 ggttggcatt atcgccaatg accgtatagt tgatgcgttg cgctgagcca agcagcccta
      481 caaacacctc gcccgagttg atgcctactc ccatttcgat ctggctgggg cgaccttctt
      541 ttgcccattt atctcgaagc tcagccaatg ctttgcgcat atccattgag gcgcgcagag
      601 cacgaaccgc gtggtcttta tagggaacgg gttcgccaaa gaacgcaatg atggcatcgc
      661 cttcgtattt atctacggtg ccgccatgtc tataaatgac ctgcgacatg gcttcgaggt
      721 aggggttaag cagggcaacc actccttccg gcgtcatttt ttcagaaagt gtcgtgaagc
      781 cgcgaatgtc tgaaaagata atggaaagat ctgcacgttt gttgagtgaa ttaatatctt
      841 gagtcttcat catctggtca accatttctg gagaaatgaa acggctgaat agattacgaa
      901 cacggcgctt ttcctgttcc tgagaaactg cctgctctaa tgtgggaagg ataacgccaa
      961 ggaaaagcat aagggttggc gcaatggttg gcaatatata acgttgactg gagaaaacaa
     1021 aatacgcagc aaccaaatag agaagaatgc taatgcctaa agcggtgagt gtaaaagacg
     1081 ggcgctttga aagcgtgata aggtatgcca taccagccgc cacgattatt agcaaaagtg
     1141 ccaaccaagg tggggcatag gtaatgaact tgccattgat gattgtatct accgtgttcg
     1201 caacgacttc cacaccaggt gttggatctg tcgccgaaaa gggggttggg taaatatcct
     1261 gtaatgtaat cgtcgtcgcg ccaatcaaga caattttgcc ccgaaaggca tccgggttct
     1321 gttccaacgt gatgccatcg tgaacatctg atgcagaata ggttgggtag gttccggcag
     1381 gtcctgcaaa atagatagga agttcattgg atcgcaacgg aatgttttga ccgttaaatt
     1441 taacgcttga gtttccaaat attggttctt cagtattcaa atataacttt gcaagttcaa
     1501 acgcccaatg cggataaacc tgtccctgat
//
LOCUS       DAOREF010000826         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_77817_length_1519_cov_5.207650, whole genome shotgun sequence.
ACCESSION   DAOREF010000826 DAOREF010000000
VERSION     DAOREF010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77817_length_1519_cov_5.207650"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PKI33_14675"
     CDS             complement(<1..731)
                     /locus_tag="PKI33_14675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM38311.1"
                     /translation="MTEDPNRALLELLVKVSREVATALDLRTVLQRVLYACIQYVGGE
                     RGSIVVMDDLGKPVDATIVYGKQFHDHTTQQLRETVERGLAGWVVQNRKPALVPDTSK
                     DERWLRRADDAAEKSGAKSAICVPLLARERFVGVLTLVHPVPNAFNEQHLELVQAISD
                     QASIAVLNARLYTETQRTARVMTALAEGAATINSSLEMQDVLRRILNQTMQALQVETV
                     ALGLIDSHSNDLVFQAAAGNNSGHIP"
     gene            775..1452
                     /locus_tag="PKI33_14680"
     CDS             775..1452
                     /locus_tag="PKI33_14680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNM38312.1"
                     /translation="MPLDVYRINALCFDVDGTLSDTDDLYVQKVSKLFPRFLFKDPEH
                     AARRFVMWIEAPGNALLGLADTLHLDDEMVAVINWLSRHRKQSDKKFLLVPGVDEMLK
                     QLHGRYPMAVVSARDEKGTMRFLEQYDLVKYFDAVITGLSAVHTKPYPDPIFLAAQKM
                     NVSPENCLMIGDTTVDIRAGKSAGAQTLGVLCGFGEEPELRHLGADEIVEDTTKLLEI
                     LTINRER"
BASE COUNT          374 a          388 c          369 g          388 t
ORIGIN      
        1 ggaatatgcc cagagttatt tccagctgcg gcttgaaata cgagatcatt gctatgcgag
       61 tcgatcagcc ccagcgcaac cgtttccact tgcaaggctt gcatggtctg attcaagatg
      121 cgacgtaata catcctgcat ttccagcgaa gaattgatcg ttgcagcgcc ttctgccagc
      181 gccgtcataa ctcgcgccgt gcgctgggtc tcggtataca agcgggcatt caacactgca
      241 atgctcgcct gatcggagat cgcctgaact aattcaagat gttgttcatt aaaagcattg
      301 ggaacagggt gaaccagcgt caacacacct acaaagcgtt cgcgcgccag aagcggcaca
      361 cagatcgcag acttggctcc acttttttcg gcggcatcat ctgcacggcg tagccaacgt
      421 tcatccttgc tagtatcagg gacaagggct ggtttccgat tttgaaccac ccaacctgcc
      481 aacccacgtt cgaccgtttc tcgaagttgt tgtgtggtgt gatcatggaa ttgttttcca
      541 tacacaatgg tggcatctac aggtttgcca aggtcatcca tcaccacaat gctgccacgc
      601 tcaccgccca cgtactggat gcaagcgtac agaacccgtt gcaacaccgt gcggagatcc
      661 agcgcggtgg caacctcacg gctgaccttt actaataatt cgaggagggc acgattagga
      721 tcttcagtca ttcttatccc ggcttaagta tagccccttt ggtacaatta atccatgcct
      781 ctagacgtat ataggataaa tgcactatgc ttcgatgtag atggcacctt aagcgataca
      841 gatgacctgt acgttcaaaa ggtgtcgaaa ttgtttcccc gttttctttt taaggaccca
      901 gaacatgccg cacgtcgctt cgtgatgtgg atcgaagctc ccggcaatgc cttgcttggt
      961 cttgccgata cgcttcattt ggatgatgaa atggtggctg tgatcaattg gctgagtcgt
     1021 caccgcaaac aatctgataa aaaattccta ctcgtccctg gtgtggatga gatgctcaaa
     1081 cagctacacg gacgttaccc catggctgtc gtcagcgccc gagatgaaaa agggaccatg
     1141 cgttttctcg agcagtatga ccttgtgaaa tatttcgatg ctgtgattac agggctttca
     1201 gcggttcaca ccaaacccta tcctgatccc atctttctgg cggcgcagaa gatgaacgtc
     1261 tcgccggaaa actgtctcat gattggggat acaacggttg atattcgtgc gggcaaatcg
     1321 gctggcgcac aaacccttgg cgttctatgt ggttttggcg aagagccaga gctaagacat
     1381 cttggcgccg atgagattgt agaagataca acaaagctat tggagattct gactataaac
     1441 cgtgagcgct agaccttcgt ctagcgctca cattctttcg ttatgagttc ttaatcactc
     1501 ttggggcaaa taagaacag
//
LOCUS       DAOREF010000827         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_77826_length_1519_cov_3.577869, whole genome shotgun sequence.
ACCESSION   DAOREF010000827 DAOREF010000000
VERSION     DAOREF010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77826_length_1519_cov_3.577869"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKI33_14685"
     CDS             complement(<1..194)
                     /locus_tag="PKI33_14685"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HNM38313.1"
                     /translation="MTEKPTGITANKSKREFIVNWDSGHVSAYPFGLLRAACPCASCR
                     GGHENMKSEPDADVFTKQLE"
     gene            complement(226..702)
                     /locus_tag="PKI33_14690"
     CDS             complement(226..702)
                     /locus_tag="PKI33_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNM38314.1"
                     /translation="MDLKPYLNRFVIVGSLITAGILILITLILIGWTSPRFSPEVGFA
                     PADLTMIPAPTHTPAASPTPTLDPFAPPTVDATTIHIDGYVQIIEVGTDGLRIRSAPG
                     LNTETVFLGGESEVFLVKDGPQDADGYTWWYLVASYDDTRAGWAASEFLTVVPPPQ"
     gene            complement(704..>1519)
                     /locus_tag="PKI33_14695"
     CDS             complement(704..>1519)
                     /locus_tag="PKI33_14695"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNM38315.1"
                     /translation="THAVQTAHQAAQEKYDAVFAIGGDGTVGQVASGLIHTETALGVL
                     PAGTVNVWALEQGQKPYSWWQPGNLKENAKLLSNVEPQYVDVGDCNDQPFLLWAGVGL
                     DAHTINKIEPRPRIFKHIAFPHYFVTTVWEATFWHGLDLRIYAEGKLVEGHYLVAVAT
                     NIRHYAGGQSLISPNALLDDNEMDLWLLSGSNVADALRHYFDLVAGRHLTSEEARCIP
                     FHHVTIESDANFSIQVDGDPMLGGHKAEIKVKHRALKVLVPENALYLLKTPQR"
BASE COUNT          354 a          354 c          416 g          395 t
ORIGIN      
        1 tcttccaatt gttttgtgaa cacatcggcg tcgggctccg atttcatatt ctcgtgacca
       61 ccgcggcagg aggcgcaggg gcaagcggct cgtaataacc caaagggata tgcactgacg
      121 tgcccgctat cccagttgac gataaattca cgtttgcttt tgttggctgt aattccagtt
      181 ggtttttcgg tcatattatt cctttgatta tttcgatcaa taattttatt gcggcggtgg
      241 gacgactgtc agaaattcgg aggctgccca gcctgcacgg gtatcgtcgt acgatgcaac
      301 gagataccac catgtgtagc catctgcgtc ttgaggtccg tctttgacga gaaagacttc
      361 tgattctcca ccaaggaaga cggtttcagt attaagtccg ggtgcagagc gaatgcgcaa
      421 cccgtctgtg ccgacttcga tgatttgcac atatccgtca atgtggatgg tagtggcatc
      481 aaccgtggga ggagcaaaag gatcgagtgt aggcgtgggg ctggcggcgg gggtgtgggt
      541 tggggcgggg atcatggtta ggtctgctgg agcaaaccca acttcgggag agaagcgggg
      601 cgacgtccaa ccgatgagaa tgagcgtgat cagaatcaaa atcccggcgg tgatcaatga
      661 tcccacaatg acgaagcgat ttagataggg ttttaagtcc atattatctc tgcggtgttt
      721 tcagcaagta taaagcgttt tcgggtacta acactttgag cgcccggtgc tttactttga
      781 tctctgcttt gtgcccgcca agcatggggt cgccatctac ttgaatggag aagttcgcat
      841 cagactcaat ggtgacatga tggaacggga tgcagcgtgc ttcttcagag gtgagatgtc
      901 tgccggctac aaggtcaaag tagtgacgca aggcatcggc aacattgctg ccgcttaata
      961 gccataagtc catttcatta tcatcgagca gagcattggg agagataaga gattgtccgc
     1021 ccgcatagtg ccgaatgttg gtggctactg caaccagata atgcccttcc actaactttc
     1081 cttcggcata aattcgcagg tctaacccat gccagaaggt ggcttcccag acggtggtta
     1141 cgaaataatg gggaaatgca atatgcttga aaatgcgcgg acgtggctcg atcttgttga
     1201 tggtgtgtgc gtctaagcca acgcctgccc acaatagaaa aggctgatcg ttgcaatcgc
     1261 ctacatctac atattgcggt tcaacatttg acagtaactt tgcgttctct tttaaattgc
     1321 caggctgcca ccaactatag ggcttttgcc cttgctctaa tgcccaaaca tttacggttc
     1381 cagcagggag tacgcccagc gcggtttcgg tgtggatcaa accgctggca acttgcccta
     1441 cggtgccatc gccaccaatg gcaaagaccg catcatattt ttcttgtgcg gcttggtgcg
     1501 cggtttgtac agcatgggt
//
LOCUS       DAOREF010000828         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_77828_length_1519_cov_3.510246, whole genome shotgun sequence.
ACCESSION   DAOREF010000828 DAOREF010000000
VERSION     DAOREF010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77828_length_1519_cov_3.510246"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKI33_14700"
     CDS             complement(<1..629)
                     /locus_tag="PKI33_14700"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNM38316.1"
                     /translation="MNKRAVVLYLSIILGLVLIVTGWYAASFLTYTSESVPEGFSGDR
                     AYVDVQTQVDMGPRTPGSAGHAQIREWMRTELESAGWIVEVHESERLGHPIYNIIAKR
                     GKEPPQIILAAHYDTRFFADNDPDPAKHTEPVPGANDGASGVAVLLELARTLPADTTP
                     TWLVFFDTEDNGRVEGWDWILGSRAFVEEVPIQPQAVVIIDMIGDADLN"
     gene            complement(629..>1519)
                     /gene="trmFO"
                     /locus_tag="PKI33_14705"
     CDS             complement(629..>1519)
                     /gene="trmFO"
                     /locus_tag="PKI33_14705"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNM38317.1"
                     /translation="DEHLFFFDAISPIVRAESINMDIAFRGSRYDKSEQEEGDYINCP
                     FTKEEYYEFLTALKTAERIELRAFEDAIKTGVKAGQFFEGCLPVEIIAERGDESLAYG
                     PMRPVGLRDPRTGKRPYAVAQLRQDNLAGSLYNIVGFQTNLKFPEQRRVLRMIPGLAN
                     AEFERYGQMHRNTFIASPKLLRPTLQHITRDDLFFAGQLTGVEGYMGNIATGLLAGIN
                     MARFIQNKALLTLPKESMLGALCHYITHADLKDFQPMKANFGILPDLNLGKKISKREK
                     GQAYADRASQSLETYLQESS"
BASE COUNT          357 a          372 c          397 g          393 t
ORIGIN      
        1 atgttcaaat ctgcatcgcc gatcatatca ataataacca cggcttgtgg ctgaatgggc
       61 acttcttcta caaaagcgcg tgagccgaga atccaatccc agccttctac acgaccgttg
      121 tcttcggtat cgaaaaagac caaccatgtg ggtgtcgtat ctgctggcag ggtgcgtgct
      181 aattctagca atactgccac acctgaagct ccatcatttg cgccggggac aggttctgta
      241 tgcttggcgg ggtctgggtc attatctgcg aagaagcgtg tatcgtaatg agcggcgaga
      301 atgatctgtg gtggttcttt tccgcgtttg gcaatgatat tgtaaattgg atgccccaga
      361 cgctctgact cgtggacctc tactatccat ccagcggact ctagctctgt tcgcatccat
      421 tcccggattt gggcatgtcc cgccgaccca ggcgtacgcg gacccatatc cacctgggtt
      481 tgcacatcca cataggcgcg gtcgccgcta aagccctcag gaacagactc agacgtgtag
      541 gtcaagaaag aagcggcgta ccatccggtg acgattaaca ccaagccaag gatgatggat
      601 agataaagaa cgacggcgcg tttattcatc aagaactttc ttgtaaataa gtttccaacg
      661 attgtgaagc acggtctgcg taggcttgcc ctttttctcg tttgctgatt ttcttgccga
      721 gattcaagtc aggcaaaatt ccaaagttgg ctttcatcgg ttgaaagtct ttcaaatctg
      781 catgggtgat atagtgacac aacgccccaa gcatcgattc tttcggaagc gtgagcaagg
      841 ctttgttttg aatgaacctt gccatgttga tacctgctaa caagcccgta gcgatgttgc
      901 ccatgtagcc ttccacaccg gtgagttgac ccgcgaagaa aaggtcgtca cgtgtgatgt
      961 gttgaagggt gggacgcaaa agtttcggcg atgcaatgaa ggtgttgcgg tgcatttgcc
     1021 catagcgctc gaactccgca ttggcaagac cgggaatcat gcgcaataca cggcgttgtt
     1081 cggggaattt caaattggtt tggaagccga cgatgttata taaacttcca gcgagattat
     1141 cttgccgcag ttgcgccact gcatatggac gtttgcccgt gcgtgggtcg cgcaagccga
     1201 ccgggcgcat gggtccatac gccagcgatt cgtcgccacg ctcggcgatg atttcgactg
     1261 gcaaacaacc ctcaaagaac tgacccgctt ttacgccggt tttgatagca tcttcaaagg
     1321 cgcgcaattc aattcgttct gccgttttta aggcagtcaa aaattcgtaa tattcttctt
     1381 tggtgaacgg acagttgatg taatcgcctt cttcctgttc gcttttgtcg taacgcgaac
     1441 cgcggaaggc gatatccatg ttgatgcttt cggcgcggac gatgggtgaa atggcatcga
     1501 agaagaagag gtgttcatc
//
LOCUS       DAOREF010000829         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_78043_length_1517_cov_2.733926, whole genome shotgun sequence.
ACCESSION   DAOREF010000829 DAOREF010000000
VERSION     DAOREF010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78043_length_1517_cov_2.733926"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKI33_14710"
     CDS             complement(<1..535)
                     /locus_tag="PKI33_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNM38318.1"
                     /translation="MSDIVQPPKPVTEIWKPELVRLPELTSLRLAFRRFAYGLVKLVV
                     WSCLRVTREGLENIPSSGPLLIVINHLGHADVPALISSLPYTPDALGEIELYDVPVLG
                     RLMDWYGVIWLHRGRPDKRALRAALDGLAEGRVIVIAPEGRYSLTGALEEGTGGAAFL
                     ARKSGAPILPIAVIDTEN"
     gene            complement(539..1219)
                     /gene="cmk"
                     /locus_tag="PKI33_14715"
     CDS             complement(539..1219)
                     /gene="cmk"
                     /locus_tag="PKI33_14715"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNM38319.1"
                     /translation="MTKTPPSIIALDGPAASGKSTVGRRLADALGYLFFDTGVMYRAV
                     TWIALNHDMDLKNEAAITQMAESAELDIRPPSQNDGRACDIIVGDKDITWDVRSGDVE
                     ASVSLVAAYAGVRTALSGQQRRIGLRGKVVMVGRDIGTVVLPEADLKVYMDASAEERA
                     RRRFKDITSRGEQANYDELLKKMIERDRLDSTRAVAPLRPADDALVLDTDKLSEEEVF
                     AKILGMCK"
     gene            complement(1314..>1517)
                     /locus_tag="PKI33_14720"
     CDS             complement(1314..>1517)
                     /locus_tag="PKI33_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38320.1"
                     /translation="FVITSYELDPQLVSAYRGQDFTWRQAPLWNGADALAWLRWVSLR
                     QMPQGQETILLWARTDLFLGGKQ"
BASE COUNT          388 a          429 c          374 g          326 t
ORIGIN      
        1 tattctctgt atcaatcacc gcaatcggca aaatgggtgc gccagattta cgcgccaaga
       61 aagccgctcc gcccgtccct tcttccaatg cgccagtcag cgagtaacgc ccctcgggtg
      121 caatgacgat gacacgccct tctgccaacc catcgagcgc ggcacgtagg gcgcgcttat
      181 caggtcgccc acggtgcagc cagataacac cataccaatc catgagccga cccaagaccg
      241 ggacgtcgta caactcaatt tccccaagtg catcgggcgt atagggcaag gacgagatca
      301 atgctggcac atcggcatga cctagatgat tgataacgat cagcaaaggt ccgcttgacg
      361 ggatattttc caacccttcc cgtgtgacgc gcaggcaact ccatacaacc agtttgacca
      421 atccatatgc gaacctgcga aaggcgaggc gcagagatgt caactcgggc agccgcacta
      481 attccggctt ccaaatttca gtgacgggtt taggcggctg cacaatatca gacatgcttt
      541 atttgcacat ccctaaaatc ttagcaaaga cttcttcttc cgacaattta tccgtatcca
      601 ataccagcgc atcgtcggca gggcgcagcg gagcgacggc gcgcgtggag tctaagcggt
      661 cacgctcaat catctttttg agaagctcgt catagttagc ttgttcgcca cgggaagtaa
      721 tatctttaaa acggcggcga gcacgttctt cagcgctggc atccatatac actttcaggt
      781 ccgcttcggg taaaacgacc gtaccaatat ctctgccgac catcaccacc ttaccacgca
      841 acccaatgcg gcgttgttga ccagagagtg cagtgcgaac ccctgcatat gccgccacta
      901 atgagacact agcctccaca tctcccgaac ggacgtccca cgtaatgtct ttatcgccca
      961 cgataatatc gcaggcacga ccgtcgtttt gagagggcgg gcgaatatca agctcggcag
     1021 actctgccat ttgggtaatg gcagcttcgt tttttaggtc catatcgtga ttgagcgcga
     1081 tccacgtcac agcgcgatac atcacacctg tatcaaagaa taaataaccc aatgcatctg
     1141 cgagccgccg ccccactgta gattttccag aggcggctgg tccgtcgaga gcaataatgg
     1201 aaggaggagt ttttgtcata gtgttcccat tttacatcag acaataaagc agccacagag
     1261 ggcattgagc tttgaataaa aaaagaccgt aggggttcta cggtctttga cgtttactgt
     1321 ttaccaccga ggaaaaggtc ggttcgtgcc cagaggagaa tggtctcttg accttgcggc
     1381 atctgacgca gcgaaaccca acgcaaccat gcaagagcat cagccccatt ccaaagggga
     1441 gcctgccgcc aggtaaagtc ctgtccgcga taggctgaga ccagttgggg atccagttca
     1501 taggaggtga tgacgaa
//
LOCUS       DAOREF010000830         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_78239_length_1515_cov_7.146575, whole genome shotgun sequence.
ACCESSION   DAOREF010000830 DAOREF010000000
VERSION     DAOREF010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78239_length_1515_cov_7.146575"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKI33_14725"
     CDS             <1..489
                     /locus_tag="PKI33_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38321.1"
                     /translation="NLLIRIESLTISPSASTGQVLDRGDICILCDDRRLELNGMIALA
                     DGNNSIGDAHPPSHGAGHVLFGLCPTYTKCRNEGTYTTNSGWWMEMDYPVTNRPLSFT
                     TSVIDYDTENSEDLFCVALAVLPARSAQEWSRANETVVLQSDFGEATCRFQITVTGQT
                     GN"
     gene            501..1451
                     /locus_tag="PKI33_14730"
     CDS             501..1451
                     /locus_tag="PKI33_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38322.1"
                     /translation="MKRIFLFVLIAILLTACAGGETTSSSGVQAWIDQPVWGTVLPVG
                     AFTLKAHARHTSGSGVTKIEFLVNGVSVGSVDTDPSAPLVYAETSWNASTTGVYQVSV
                     MAYAGAESSESAIVTVCVSQEITVAGVASDGDCAASQPIQPSTSEDATASPVPQNPNT
                     TSTVTPTATFTSIPPSAVVLPTITFTPNPPTDTSVPVDMQGPSIKSLYHSAPGYYGGC
                     SGVFTMQAIGVSDPSGIASVIFGYRYEGSSSSGYFTATGVPVGNDTYELTIDSNAGSQ
                     AYNTLQGANGFIRWYVQVQDGAGNISTISDQVGEMLFCPG"
BASE COUNT          379 a          358 c          395 g          383 t
ORIGIN      
        1 aatcttttga tccggatcga gtcactcacc atcagcccta gtgcatctac agggcaggta
       61 ttagaccgcg gcgatatctg tattttgtgt gatgaccgtc gcctcgaact gaacggtatg
      121 atcgccctcg cagacggcaa caattccatt ggcgatgccc atccacccag ccatggagcg
      181 gggcatgtgt tatttggact gtgtcctact tatacaaaat gccgcaacga agggacgtac
      241 accacaaact ccggttggtg gatggaaatg gattatccag tgacaaaccg tcctttgtca
      301 ttcacgacat ctgtgattga ttacgacacc gaaaatagtg aagacttgtt ctgtgttgcc
      361 ttggcggttt tgcctgccag gtcggctcaa gaatggtcac gtgccaacga aacagtagtt
      421 ttgcagagtg attttggtga agccacctgc cgcttccaaa ttacggtcac cgggcaaaca
      481 gggaattaag aggaatagcc atgaaacgaa tctttctttt tgtcttgatc gctatcctat
      541 tgacagcatg tgcgggcggc gaaacaacat cttccagtgg tgtgcaagcg tggatcgatc
      601 aacctgtttg gggaacagtt cttccggtgg gagcatttac cctcaaagcc catgctcgcc
      661 atacctctgg cagcggggtg acgaagatcg aatttctggt gaacggtgtg agtgtaggct
      721 ccgtggatac tgacccatct gctccgcttg tatatgcgga gacatcttgg aatgcatcga
      781 caacgggggt atatcaagtg agtgtaatgg cgtatgctgg tgcagaatct tctgaaagtg
      841 cgatcgtaac ggtctgtgtt tcgcaagaaa tcaccgtagc aggagtggca tcagatggag
      901 actgtgctgc cagtcaaccg attcagccgt ccacaagtga agatgcgaca gcttcacctg
      961 tgccgcaaaa tcccaatacg acgagtacag ttacgcccac ggctacattc acatccattc
     1021 cgcctagcgc ggttgttcta cccacaataa cttttactcc aaacccgcct acggatacat
     1081 cggttccggt agatatgcag ggacccagta tcaagagctt atatcacagt gctccggggt
     1141 actacggagg ttgcagcgga gtatttacca tgcaagccat tggggttagc gacccatcgg
     1201 gtattgcttc ggtcatcttt gggtatcgtt atgaaggtag ttcttccagc ggatacttca
     1261 ctgccaccgg tgtgccggtt ggaaatgaca cctacgaatt aacgatcgat agtaatgctg
     1321 gcagccaagc gtacaacaca ttgcaaggag ccaatggttt catccgatgg tatgttcaag
     1381 tgcaagatgg tgctgggaat atatccacga ttagtgatca agtaggtgaa atgttgtttt
     1441 gcccaggata aggagataat aaaaagagca tccagatgga tgctcttttt attatgaagc
     1501 gcgcttggct ccggc
//
LOCUS       DAOREF010000831         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_78859_length_1509_cov_4.863824, whole genome shotgun sequence.
ACCESSION   DAOREF010000831 DAOREF010000000
VERSION     DAOREF010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78859_length_1509_cov_4.863824"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1509)
                     /locus_tag="PKI33_14735"
     CDS             complement(<1..>1509)
                     /locus_tag="PKI33_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38323.1"
                     /translation="IKESEVLPSYEKILKEQGIVPSRYVLSNAIAATQSLGHENLGFL
                     SETHGFMPSQPPLLKLPPAYQAWDDIVDNLPDLYRTLGLRAALEQMPTLSADPETLPD
                     KYLLRASMIISILGHAYFRIQTDAPQKLPESIQQPWQKITQRLGRRGPVLSYIDLIIY
                     NWKLIDPALQDPIIVENMRLMVPTVDNIVERIFYLGQVEILSRLTPVIGATVRAQEAV
                     YRDDPDALKQELVSITEALHRTTYHSLQKIDLNPHNKAYHLDTVVWAKTVGPLAVSLE
                     ESTPGPSGIASPIFHLLDEFLGRQTYATRLGEEMTRIREWYPPNWQNFLKAIGQISVA
                     GYVEKSRSKTLKGIFQETRQAYVAETGFLGRHRLKAYGFLETAFKVGRSVTIGSFSGK
                     FKDRAWDEVHAQLNNSQLERSSGHPQYYHYADVKAVNTLTADGENWVKQVVLNTSGTG
                     ISYQVGDRAALLPENSDALIDKTLQALRARGREAIALNKAWREGILQREGYK"
BASE COUNT          335 a          380 c          398 g          396 t
ORIGIN      
        1 ctttatatcc ttcgcgttgt agaatgcctt cacgccacgc cttattaagg gcgatggctt
       61 cgcgtccgcg ggcgcgtagg gcttgtaacg ttttatctat caacgcgtca ctattctctg
      121 gcaagagtgc ggctcgatct ccaacctgat aactgatgcc cgttcctgat gtattgagca
      181 ccacctgctt cacccagttt tccccatccg cagtcaatgt attgacggct ttcacatcgg
      241 cataatggta atactgcgga tgcccgctgc tgcgctccag ttgtgaattg ttcaactgtg
      301 catggacttc atcccacgcc cgatctttga acttgcccga gaagctgccg atcgtaacgc
      361 ttctacctac cttgaaggct gtctctaaaa atccataggc cttcaaacga tgccgaccca
      421 ggaaacctgt ctctgctaca taggcttgcc gtgtttcctg gaagatgccc ttgagcgttt
      481 tacttctgct tttttcaaca tatcctgcca ccgaaatttg cccgatggct ttgaggaagt
      541 tctgccaatt gggcggatac cactcgcgaa tgcgcgtcat ctcttcgccc agacgggtgg
      601 cgtaggtctg acgcccgagg aattcatcca gcagatggaa gatgggcgat gcaattccac
      661 ttggaccggg tgtgctttct tccagtgaga ccgccaaggg accgactgtt tttgcccaga
      721 cgaccgtatc caggtgatac gctttattat gcggattgag atcgatcttc tgcagggaat
      781 gataggtggt gcggtgcaag gcttcggtga tggacaccaa ttcctgcttg agtgcatcgg
      841 gatcatcgcg atagactgct tcttgcgcgc ggacagtcgc cccaatgacg ggtgtgagtc
      901 gcgagagaat ttcaacctgc cccaggtaga agatgcgttc cacaatattg tcgactgtgg
      961 gaaccatcaa gcgcatgttc tcaacgatga tggggtcttg gagagcgggg tcgatcaatt
     1021 tccagttata gatgatcagg tcaatatacg agagaaccgg tccgcgcctg cccaggcgtt
     1081 gtgtaatttt ttgccagggt tgttgaatgc tctcgggtaa cttctgcggc gcgtccgttt
     1141 gaatgcggaa ataagcatgc cccagaatgc taatgatcat ggaggcacgc aagagatatt
     1201 tatctggaag ggtttctgga tcagcgctaa gggttggcat ctgttccaga gcggcacgca
     1261 gcccaagggt ccgatataga tccggcagat tgtctacgat gtcatcccag gcttgatagg
     1321 caggaggcag tttcagcaaa ggcggctggc tgggcataaa tccatgtgtt tctgaaagga
     1381 aacccaaatt ctcatgccca agactttgag tggctgcaat cgcattcgaa aggacatagc
     1441 gcgaaggcac aatgccttgc tctttcagaa ttttttcata cgatggaagg acttcagatt
     1501 ctttgatct
//
LOCUS       DAOREF010000832         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_78981_length_1508_cov_2.960771, whole genome shotgun sequence.
ACCESSION   DAOREF010000832 DAOREF010000000
VERSION     DAOREF010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78981_length_1508_cov_2.960771"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..277)
                     /locus_tag="PKI33_14740"
     CDS             complement(107..277)
                     /locus_tag="PKI33_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38324.1"
                     /translation="MNFNKRRVSGYLLIFGMAFLVIGITTDNTIFSWISVGFIVISLF
                     LGGRWMRQRRRP"
     gene            complement(292..>1508)
                     /locus_tag="PKI33_14745"
     CDS             complement(292..>1508)
                     /locus_tag="PKI33_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38325.1"
                     /translation="TPLLALSSLSKLFGTSDWKPIASYLTVGLVALATALPILLPVPR
                     IPTPSGPFQVGTRSFEFLDESRQEIYSGKDEARRFMIQVWYPADAKASDVRAPWMENA
                     EVFAPAIAGFFKLPSFFFDHLTLTSIPAFMDADVASADEKFPVILFSHGWNGFASQNA
                     GQALELASRGYVVVGLQHTYGAMTTVFPDGTIAPNNPKALPEDGADPNYEEVARVLVD
                     QWAGDMAYTLDQLTAWNDAEGNPFFSKLDMERVGVYGHSTGGGAAIQFCGTDSRCDAV
                     LGLDPFMRPVSAEVLASGVSQPSFFMFSQAWADDANSPSSILFGQFIPHAADNYGVVK
                     IEGTRHYDFSDLPLFSPIAPQLGLKGPLNGKRVTEIVNSYLIDFFEMTLKNKPSELFS
                     NVSPFSEVVPIP"
BASE COUNT          428 a          389 c          327 g          364 t
ORIGIN      
        1 atttcacggt gaagcctaat acctctgtgt agaacttttt ggacctttca tagtccgagc
       61 aaataatagc cacatgatga atatgcttta tattcatctt aaacctttat gggcgtcttc
      121 tttggcgcat ccaacgtccg cccaaaaaca aggagatcac tataaaccca accgatatcc
      181 aagagaaaat tgtattatca gtagtaatac cgattaccaa aaaagccatg ccgaagatca
      241 acaaatatcc cgaaactctg cgtttattga agttcatact acctctcacg attatggaat
      301 aggaactact tctgaaaatg gactgacatt gctaaaaagc tcagaaggct tgtttttcaa
      361 ggtcatctca aagaaatcga ttagatacga attgacgatc tcagtcacac gttttccatt
      421 tagtggaccc ttcagcccaa gttgtggcgc gatcggcgag aacaatggca agtcgctaaa
      481 atcataatgc cttgttcctt caattttgac aaccccataa ttatctgccg cgtgtggaat
      541 aaattgacca aacaaaatac tgctggggct attcgcatca tctgcccagg cttgtgagaa
      601 cataaagaat gagggttggc ttacaccact tgctagcact tctgctgaaa ccgggcgcat
      661 gaagggatcc aagccgagca ctgcgtcaca gcgcgagtct gtgccgcaaa attggatcgc
      721 cgctccgccg ccagtagagt gaccatatac accgactcgc tccatatcta gtttgctgaa
      781 aaacggattt ccctccgcgt cattccaagc tgtaagttgg tccaacgtgt atgccatatc
      841 tcccgcccat tgatccacca agacgcgcgc tacctcttcg taatttgggt ctgctccgtc
      901 ttcgggcaat gccttcgggt tgttcggggc aattgttcca tcggggaaga ctgttgtcat
      961 ggcaccatac gtatgttgga gccccaccac cacataacca cggctggcaa gttccaatgc
     1021 ttgcccagcg ttctgagaag caaacccatt ccaaccatgt gagaacaaga tcactgggaa
     1081 tttttcatct gccgaagcga catccgcatc cataaaagcc ggaatactcg tcaaggtaag
     1141 gtggtcaaaa aagaatgacg gcaacttaaa gaaacctgca atagcaggcg caaagacctc
     1201 agcattttcc atccacggtg cccgcacatc acttgccttc gcatctgccg ggtaccaaac
     1261 ttggatcata aatcgacgtg cctcgtcttt accagaataa atttcctggc gtgattcatc
     1321 taaaaattca aagctgcgtg tgcctacttg gaaaggtccg ctaggggtgg ggatgcgcgg
     1381 aacaggcaac agaataggca gagccgtcgc gagcgcaacc aagcccaccg ttaagtaaga
     1441 ggcgatgggt ttccaatcac ttgtcccaaa aagtttgctc aaactactca aggcaagcag
     1501 cggcgtca
//
LOCUS       DAOREF010000833         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_78985_length_1508_cov_2.923606, whole genome shotgun sequence.
ACCESSION   DAOREF010000833 DAOREF010000000
VERSION     DAOREF010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78985_length_1508_cov_2.923606"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKI33_14750"
     CDS             complement(<1..869)
                     /locus_tag="PKI33_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNM38326.1"
                     /translation="MTELSTTYLGLKLKNPLVASASPLSKKTDKAKKLEEAGISAIVM
                     YSLFEEQIIHESLELDHFLSRGTDSFAEALTYLPDGGMYSVSPEKYLNQVTSLKKAVN
                     VPVIGSLNGVSKGGWTSYAKRIEEAGADALELNLYFLSTDPNLTAAALETAQAELVAE
                     VKSSIKIPLAVKLSPFVTSLPNFAKRIVDAGADGLVLFNRFYQPDFDLDNLDIIHSLD
                     LSTSADLRLPLRWISILHSNVHTDYALTSGIHTAHDVLKAMMAGAKVAMMASTLLHSG
                     ETVISSMLDDLQT"
     gene            complement(932..>1508)
                     /locus_tag="PKI33_14755"
     CDS             complement(932..>1508)
                     /locus_tag="PKI33_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNM38327.1"
                     /translation="NILVLDTEVYSNTGGQSSKSTPRAAVAKFSMGGKGMPKKDLGLI
                     AMSYGYVYVAKVSMGANDQQTLKALLEAESYDGPSLVIAYSPCIAHGFDMARSLDQAK
                     LAVQSGHWPIFRYDPRLAIDGKNPLQIESKEPSIPFTQYAYNETRYKMLTQMNEERAE
                     ELMKEAQHDAKTRWTLYQQMASMHYESDKKE"
BASE COUNT          345 a          326 c          429 g          408 t
ORIGIN      
        1 caggtttgca agtcatctag catggaagag ataactgtct cgccgctgtg cagcagggta
       61 gatgccatca tggcgacctt tgcgcctgcc atcatggctt tgagtacgtc atgagcggtg
      121 tggatgccgc tggtaagggc gtaatctgtg tggacgttgc tgtgcaaaat tgagatccag
      181 cgcaggggga ggcgtaagtc tgcagaggtg ctcaaatcca agctgtggat gatatccaga
      241 ttatcgaggt cgaaatcggg ttggtagaag cggttgaaga gcacaagtcc gtctgcgccg
      301 gcgtccacga tgcgtttggc gaaattaggt aaggatgtca cgaagggact aagcttcact
      361 gccagcggaa tcttgatgga ggattttact tccgctacta attctgcttg ggcggtttca
      421 agtgcggcgg cggttaggtt tgggtctgta gagagaaagt ataggttaag ttcgagcgca
      481 tcggctccag cttcttcaat gcgtttggcg tagctggtcc aaccgccttt ggagacgcca
      541 ttcaagctgc cgattacggg gacattgacc gctttcttca aactggtcac ctgattaaga
      601 tatttttcag ggctcacgct atacataccg ccatcaggta gataggtaag ggcttcagca
      661 aaagagtcgg tgccgcggga aaggaagtgg tctaactcaa ggctttcgtg aatgatctgc
      721 tcttcaaaga gagaatacat cacgatcgcg gagatgcccg cttcttctaa ctttttagct
      781 ttgtcggtct tcttagagag gggtgaagcc gaagccacaa gcggattttt tagttttaag
      841 ccaaggtacg tagtggaaag ttcagtcatg ggtaaagttc ctttagggag ttaagaatgg
      901 gcgagaaact ttctcgccca taatttaaag tttattcttt tttgtcgctt tcgtaatgca
      961 tggatgccat ttgttgataa agcgtccaac gggttttggc atcatgctgg gcttctttca
     1021 tgagttcttc ggctcgttcc tcattcattt gagtgagcat cttataccgg gtttcgttat
     1081 aagcatactg cgtgaatggg atgctgggtt cctttgactc gatctgaagc ggatttttac
     1141 cgtctattgc caagcgcgga tcgtaacgga agatgggcca gtgaccagat tgtacggcga
     1201 gctttgcttg gtcgaggctg cgagccatat cgaagccgtg tgcaatacac gggctgtatg
     1261 caatgacgag ggaaggtcca tcatacgatt cggcttccaa cagagctttc aaagtctgct
     1321 ggtcgttggc gcccatggac acctttgcaa cataaacgta gccgtacgac atggcgatca
     1381 gaccaaggtc tttcttgggc attcccttgc cgcccatgga gaacttggca actgctgcac
     1441 gcggtgtgga cttggaggac tgaccacctg tattcgaata cacttcggta tcgagcacga
     1501 gaatgttg
//
LOCUS       DAOREF010000834         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_79012_length_1508_cov_2.379215, whole genome shotgun sequence.
ACCESSION   DAOREF010000834 DAOREF010000000
VERSION     DAOREF010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79012_length_1508_cov_2.379215"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PKI33_14760"
     CDS             complement(<1..1133)
                     /locus_tag="PKI33_14760"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNM38328.1"
                     /translation="MQLRKGDEVLIGCPDSTSSSEPSLEVTSLGSGLSLHIFGVADMR
                     WVPTAESMLDIFSSLLTADTDLENLTAALVETQDRLVALYELTQATRRTLDMPKLLDL
                     LIQQSKHLMDVDGGFVVLKEKGKQTIIHQVSENPLQPSHAEAAASLYLRDPNRHVFKD
                     SETLPANLKNVMMVTLPVRDEISAAVGLFNKTGVFTTPDIKLAKAIAGHIGAQLENAM
                     LHKEAVERARLETEMDIARQVQTAILPQSIPQVSGVDIFATSIPAFEVGGDFFDVIDR
                     SAESLIFSLGDVTGKGMPAALLMSMAHTIIKAASRNMPFDHPHQVLNRLNHDMESDFS
                     NVGMFTTVMLGMVDCANCKLHFSNAGQSPIYYMPVNGDAVLLEA"
     gene            complement(1214..>1508)
                     /locus_tag="PKI33_14765"
     CDS             complement(1214..>1508)
                     /locus_tag="PKI33_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM38329.1"
                     /translation="LKLKGRFDAHEVEPVNAWLAEQTNANQVKLIVNLEGVNFIDSTA
                     LSTLVRGLKLCREKNGDLHVCSLQQPVRVIFELTRLDKAFDIFATEEEAAKGF"
BASE COUNT          407 a          393 c          332 g          376 t
ORIGIN      
        1 gcttctaaca gaactgcatc gccattaacg ggcatatagt aaatggggct ttgcccggca
       61 ttactaaaat gcagtttgca atttgcacag tccaccatac ctagcatcac cgtagtaaac
      121 atacccacat ttgaaaaatc agattccata tcatgattga gacggtttaa gacctgatgc
      181 ggatggtcaa acggcatgtt ccgcgatgcg gctttgataa ttgtatgcgc catgctcatc
      241 aacaaagcgg cgggcatacc tttccctgta acatcgccca aagaaaaaat gagcgattct
      301 gcggaacggt caatcacatc aaaaaaatcg ccacctactt caaatgccgg tatggatgtt
      361 gcaaatatat ccacaccact cacttgcgga atactctgcg gaaggattgc cgtctgaacc
      421 tgacgagcaa tgtccatttc ggtttcaagc cgggcacgtt ccacggcttc tttatgaagc
      481 atggcatttt caagctgcgc accaatatga ccagcaatag ctttagccaa cttgatatcg
      541 ggcgtcgtaa aaacgcctgt tttattgaaa agtcccacgg cggcagaaat ttcatcgcgc
      601 actggcaacg tgaccatcat gacattcttc aaatttgccg gtaacgtttc agagtcctta
      661 aacacgtggc gattggggtc ccgtaagtac aaactggctg ccgcctcggc atgtgacggt
      721 tgtaatgggt tttctgaaac ctgatgaata atggtttgct tgcccttttc tttcaacact
      781 acaaagccgc catccacatc catcaaatgc ttggattgtt ggatgagcaa atcaagtaac
      841 ttgggcatat ccaaggtgcg gcgtgtcgct tgcgttaact catacaaggc cacgagccgg
      901 tcttgcgttt ccaccagtgc cgccgtaagg ttctctagat ctgtatctgc cgtaagcaga
      961 ctggagaaaa tatccagcat ggattccgcc gttggcaccc aacgcatatc ggcaacccca
     1021 aaaatatgta gggacaatcc cgaaccgagc gatgtgacct caagcgacgg ctcactacta
     1081 gacgttgaat ctgggcagcc aattagaact tcatctccct ttcgaagttg cacgcaggtt
     1141 gcaccgactc gcatccaacc agatgccagg gatttgaaac tctcacggtg cttcgtagtg
     1201 atctcaggaa acactagaaa cctttcgctg cttcttcctc ggtggcaaag atatcaaagg
     1261 ctttgtctaa ccgggtcaac tcaaagatga cccgcactgg ctgttgcaac gaacaaacat
     1321 gcaaatcacc attcttttca cggcaaagct tgagtccgcg caccaatgtt gaaagcgcag
     1381 ttgagtcaat gaagttcacg ccttctaaat tgacaatgag cttcacctgg ttcgcattgg
     1441 tctgttctgc caaccaagcg ttgacaggtt ctacctcatg cgcatcaaag cgccctttga
     1501 gtttcaaa
//
LOCUS       DAOREF010000835         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_79095_length_1507_cov_3.008953, whole genome shotgun sequence.
ACCESSION   DAOREF010000835 DAOREF010000000
VERSION     DAOREF010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79095_length_1507_cov_3.008953"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1507
                     /locus_tag="PKI33_14770"
     CDS             <1..>1507
                     /locus_tag="PKI33_14770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM38330.1"
                     /translation="NAPTGYHSIDADGKFVRVNKTILDWLGFARDEMMGSPISGFMTS
                     ATQKIFSEKFSELVQHGLIKDVEIELIRKDGSILPVLLNATAIRDAAGNFVMSRSTVF
                     DNTERKQAEIELKRNVNFTSALLNAVPTPVFYKDNEGRYLGCNQSFVNMMGKTAEEIY
                     GKLPHEIWESSHADLYRQKDIELFQSNELQTYEGSIRDKHGNLHSAIFVKNVFLDESG
                     KTAGLVGAFIDITERKQAELAIRESEATYRALFENSNDGIFLMAPTGEELRANQHALD
                     LVGYTLDEYLNMGGVSQNPFTLDDDERKDADDKLAALIRGETVPLYERLFTAKDGHKV
                     PVEINLSPVRDANGNLIMVQSVVRDISQRKAAEDALRTINKELERALRVKDEFLANMS
                     HELRTPLNGILGFSEILLSGEFGSLSERQHKYISSIEGSGKHLLGLINDLLDLSKIEA
                     GKLDVMPENVIVSELCQSSLVFVKQMALKKKIHLSFEQDMNYPFIIADQRRL"
BASE COUNT          440 a          307 c          372 g          388 t
ORIGIN      
        1 aaaatgcgcc aacgggctac cattctattg atgcagatgg aaagtttgtg cgggttaata
       61 aaacaatttt agactggttg ggatttgcca gagatgaaat gatgggttcg cctatttctg
      121 gttttatgac cagtgccacg caaaagatat ttagcgagaa attttctgag ctggtgcaac
      181 atggcttgat aaaagatgta gaaattgaat tgatccgaaa agacggctcc atccttccgg
      241 ttctgttgaa cgctaccgcc attcgagatg ccgctggtaa ttttgtgatg agccgttcta
      301 cagtatttga taatacagag cgtaaacagg cagagatcga actcaaacga aatgtcaact
      361 ttaccagcgc gttgctaaat gctgtgccta cccctgtttt ctataaagat aacgaaggca
      421 gatatttggg ttgcaaccaa tcgtttgtga atatgatggg gaaaaccgct gaagagattt
      481 atggcaagct ccctcatgag atttgggaaa gctcccatgc agatttatac cgccagaaag
      541 atattgaatt attccaaagc aatgagcttc agacctatga agggagcatc cgcgataagc
      601 acggaaactt gcactcggcg atctttgtta agaatgtctt tttggatgaa agcggaaaaa
      661 cagccggctt ggtaggcgcg tttattgaca ttaccgaacg aaagcaagca gagttagcca
      721 ttcgcgaaag cgaagcaact tatcgtgcct tgtttgagaa ttcgaatgac ggtattttct
      781 tgatggctcc aacgggagaa gagctacgag ccaaccagca tgcattggat ttggtaggct
      841 acaccttgga cgaatatctg aacatgggag gggtcagcca gaacccattt accttggacg
      901 atgatgaacg aaaagacgcc gatgacaaac tcgctgcatt aatacgcggc gaaacggttc
      961 cattgtatga gcggttgttt actgcaaaag atgggcataa agtgcctgta gagatcaacc
     1021 tttcgccggt gcgagatgcc aatggcaacc tcattatggt acaaagcgtg gtgcgcgata
     1081 tttcgcaacg caaagcagct gaagatgcct tgcggaccat caacaaagaa ttggaacggg
     1141 cattacgggt gaaagacgag ttcttggcga atatgagtca tgaactacgt acccccttaa
     1201 acggcattct tggcttttca gaaattctcc ttagtggaga gttcggcagt ttgagcgaac
     1261 gccaacataa atacatctct tccattgaag gcagtggtaa acacttgctg gggctgatta
     1321 atgacctgct tgatctttcc aagatcgaag ccggtaaatt ggatgtgatg cctgaaaatg
     1381 taattgtttc agaattgtgc caatccagtt tggtgtttgt aaagcagatg gcgcttaaga
     1441 agaaaatcca tctctcgttc gaacaggata tgaactatcc cttcatcatc gcagaccaac
     1501 gccgcct
//
LOCUS       DAOREF010000836         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_79368_length_1504_cov_6.728778, whole genome shotgun sequence.
ACCESSION   DAOREF010000836 DAOREF010000000
VERSION     DAOREF010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79368_length_1504_cov_6.728778"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKI33_14775"
     CDS             complement(<1..860)
                     /locus_tag="PKI33_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM38331.1"
                     /translation="MKTPSSSTSTHHEDINDVIRRADILQRVMSTISSGLELDPLLSN
                     ILESAVKLIEATHGTIGLVGDRNGKPIIRTVATHNMPAEELGAEMAPGIGLAGKVLLE
                     KKTVRLDRYGSLEEPTLQGLAEHSVIGIPILWGEEMIGFFGIGVDARRFTDHDVETLE
                     YFAQYAAVAIHNANLFKANQHALEEMHLLYKTSQRIGLSDDVDGVIDAYLDQVAAGGK
                     YVCNICLYEFDENNERTEVIVKGRWMPETGSERLDEHLPYVQDNLDPILDAGETLIFS
                     DVRNDSRAPES"
     gene            1269..>1504
                     /locus_tag="PKI33_14780"
     CDS             1269..>1504
                     /locus_tag="PKI33_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38332.1"
                     /translation="MYICVLTSTPDDDDIPYDPSPYMNGYRWKQHHVEPTEVEKQIKQ
                     LMDDGVDVFVNLCDGTPDDALSGIALVKALEKYNA"
BASE COUNT          467 a          299 c          306 g          432 t
ORIGIN      
        1 gactctggcg ctcgcgagtc atttctgaca tctgagaaga taagagtctc tcccgcatca
       61 agaatggggt caaggttatc ttgcacgtag ggcaggtgtt catccaaacg ttctgagccg
      121 gtttcgggca tccaacgtcc ttttacgatg acttctgtcc gctcgttgtt ttcatcgaat
      181 tcataaagac aaatattgca gacatactta ccgcccgccg caacttgatc caaataagca
      241 tcgatcactc catctacatc atcggaaagt ccaatgcgtt gacttgtctt ataaagcagg
      301 tgcatttctt caagtgcatg ctgatttgcc ttgaaaaggt tagcattgtg aatggcaacg
      361 gcagcgtatt gagcgaaata ttccagagtt tccacatcat gatctgtaaa acgccgagca
      421 tctacgccaa ttccaaagaa tccgatcatc tcttcgcccc ataaaatggg gattccaatg
      481 acggaatgtt ctgcaagccc ttgaagagta ggttcttcta gtgagccata acggtctaag
      541 cgtactgttt tcttttcaag taatactttt ccagctaacc ctatccccgg agccatttct
      601 gcgcctagtt cttcggctgg catattatga gttgccactg tgcggatgat aggcttgcca
      661 ttgcggtctc caacaagccc gatcgttcca tgtgtggctt caattagttt gacggcgctc
      721 tcaaggatgt tggagagtag tgggtcaagc tccaatccgc tactgatcgt tgacatcaca
      781 cgctgaagaa tatccgcacg gcggattaca tcattgatat cttcgtgatg tgtagaggtg
      841 gatgaagaag gagttttcaa gtttacttgc tctgccaata agttttacga attatatacc
      901 aatgaataga ttgatttgaa tgaaattgta gactgaaact tatatgcaga gttggtgtta
      961 attacacaag taaacatcaa acagagacgt tctttttctt aaggacgtta ataaccttaa
     1021 aaaccttaac gactttatga ataaacatta taaaaagatg aataaagctt tcaaatattt
     1081 taagataaaa acatgtgtaa aaacctttac ataaacaaat agttgtatac aataatcata
     1141 tattgatgat aatttaaata tttttcatca tatgatgata tgtaaaacgt ttagattgat
     1201 agatagcatg cgacatgaag ataaataaaa aatcatgaac gcaaaaaagg aatgacaaag
     1261 gaaatatcat gtatatctgc gtactaacaa gcacacctga tgacgacgac atcccatatg
     1321 atccttcacc atacatgaat ggatatcgtt ggaagcagca tcatgtggaa ccaaccgaag
     1381 ttgaaaaaca aatcaaacaa ttgatggatg atggcgtgga tgtgttcgtc aatttgtgtg
     1441 atggtacacc cgatgatgca ctttcaggaa ttgccttggt caaggcgctt gaaaaatata
     1501 atgc
//
LOCUS       DAOREF010000837         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_79568_length_1502_cov_3.812716, whole genome shotgun sequence.
ACCESSION   DAOREF010000837 DAOREF010000000
VERSION     DAOREF010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79568_length_1502_cov_3.812716"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1422
                     /gene="tyrS"
                     /locus_tag="PKI33_14785"
     CDS             211..1422
                     /gene="tyrS"
                     /locus_tag="PKI33_14785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNM38333.1"
                     /translation="MNIEEQVELLMQGTDYGDEDLQKAMASELRERLKIAEKENRPLR
                     VYCGYDPTSTDLHLGHTISMRKLRQFQDLGYEVTFLIGNYTALVGDPSDKNKARPILT
                     QEQVAENAKTYAEQAFRVLDKSKTKIRYNSEWLSKLSLVDLIRLGQNFTVQQFLAREN
                     FAKRLDKGEPIYLHETFYALMQGYDAAAMETDIQVGGSDQLFNIIVAGRKLQEALGQK
                     PLVGIITGILPGTDGAQRMSKSTGNIVPINTGADDMFGKLMSVPDSAMGTYMRLVTRF
                     NPHEIAQIEKDVASGALHPRDAKMKMAQEIVSIFYTDAEAVSAQDHFIKMFQQKEVPD
                     EIPEYDLQSGQTVVDVILSAGMAESKSKARQLIDQKGVRLDGEVLERGDAAFPHPGVL
                     QVGKRRFLRVK"
BASE COUNT          433 a          322 c          358 g          389 t
ORIGIN      
        1 tcaatttgtc gcaataaaat tcctgatgta taataccgag catgtctggc aacttattca
       61 ctagtcgcta ttactattat taccacccgt aactggttgc gatggtgtga tttgaacgcg
      121 ccccatcatc gcgggcgcgt tttttgtttg tatagcgctc aaatgatata aaaaattacg
      181 acccaacagg gtttgcatta ggagaaaaga atgaacatcg aggaacaagt cgagttattg
      241 atgcagggaa ccgactatgg cgacgaagat ttgcaaaaag ccatggcatc ggaactgcgt
      301 gagcgtctaa agattgctga aaaagaaaat cgtcctttgc gggtgtattg tggttatgat
      361 ccaacgtcta cagatctgca tttgggtcat accatttcca tgcgcaaatt gcgtcagttt
      421 caggatttag gttatgaagt gaccttcctg ataggtaact acacagcttt ggtgggtgac
      481 ccttcggaca aaaataaagc gcgtccgatt ctgacgcagg aacaagttgc tgaaaatgcc
      541 aagacctacg ccgaacaagc atttcgcgta ttggataaat ctaaaacaaa aatccgctat
      601 aacagtgaat ggctctccaa actcagcttg gtcgatttga tccgtctcgg tcaaaacttt
      661 acggttcagc agttcttggc tcgtgagaac tttgccaagc gtctcgataa gggcgaaccc
      721 atttacctgc acgagacttt ctacgcccta atgcaaggat acgatgccgc cgccatggaa
      781 acggatattc aagttggtgg ttccgatcag ttatttaata tcatcgtggc tgggcgcaaa
      841 cttcaagaag ccttggggca aaaacctctg gttggcatca tcaccggcat tttgccgggc
      901 accgatggcg cacaaagaat gtccaagtct acgggcaata ttgtgcctat caataccggt
      961 gcagacgata tgtttggcaa attaatgtcc gtccctgatt ctgcgatggg aacctatatg
     1021 cgtctcgtga ctcgtttcaa tccgcatgag attgcccaga tcgaaaaaga tgttgcttcc
     1081 ggtgcattac atccgcgtga tgcaaagatg aagatggcac aagaaatcgt cagcatcttc
     1141 tatacggatg ctgaagctgt atcggctcaa gaccatttca tcaagatgtt ccaacaaaag
     1201 gaagtcccgg acgaaatacc agaatatgat ttgcaatcgg ggcaaactgt tgtagatgta
     1261 atcctttctg ctggtatggc agaaagcaaa agcaaagcga ggcagttgat tgaccaaaag
     1321 ggagttcgtc tcgatgggga agtgctagag cgtggtgatg ctgcgtttcc acacccaggc
     1381 gtgttgcaag ttgggaagcg tcgcttcttg cgtgtcaaat aaaacataaa aaggacttgc
     1441 acatgcaagt cctttttctt taagaagcta tttgataaaa caaaaaacag actcaaagtt
     1501 ga
//
LOCUS       DAOREF010000838         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_79744_length_1500_cov_6.077509, whole genome shotgun sequence.
ACCESSION   DAOREF010000838 DAOREF010000000
VERSION     DAOREF010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79744_length_1500_cov_6.077509"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKI33_14790"
     CDS             complement(<1..341)
                     /locus_tag="PKI33_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38334.1"
                     /translation="MKVYDRTPLQDAQGNINPIARVQGTLKYGLSWAGELEAQKVVIA
                     QLDRLLDKGFVLIRNFTLPDSDIVIPMILIGPGSISVILATPLKGDFEAKGNEWNSVK
                     NGVSVPASRNL"
     gene            complement(351..653)
                     /locus_tag="PKI33_14795"
     CDS             complement(351..653)
                     /locus_tag="PKI33_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38335.1"
                     /translation="MAKKVDGVIEAVRYKNGQIVSVRGYERRGFTFSDRVLLDRKTLL
                     ERLQKGQQFVAGTRQDLLASTFTLSKPILLVKADDREYIATKENVTKDELENVPLF"
     gene            complement(753..827)
                     /locus_tag="PKI33_14800"
     tRNA            complement(753..827)
                     /locus_tag="PKI33_14800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(790..792),aa:Pro,seq:tgg)
     gene            862..>1500
                     /locus_tag="PKI33_14805"
     CDS             862..>1500
                     /locus_tag="PKI33_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNM38336.1"
                     /translation="MNTRQAILKAAFSALLLGLSACSTPQTVSPTVTVTVPPPTATAT
                     LTPTPVPPTATPTPLGCLTQPGKVEQGVLTATDPEQPYIIYLPPCYSELTDQRYPVLY
                     LLHGQTYTMDQWVRLGVPQAMDDMFFSGASVPFIVVFPDDSLWNLSAGWRFGERLIGS
                     LIPYIDATYRTIPDRDHRAIGGLSRGGGWAAELGLQNPGMFISIGLHSPAIFK"
BASE COUNT          357 a          435 c          311 g          397 t
ORIGIN      
        1 atcaaatttc ggcttgccgg aacagatacc ccattcttaa cactattcca ttcattgcct
       61 tttgcttcaa aatccccttt gagcggagtt gccaagatta ccgaaatgga ccccggacca
      121 atcaaaatca tcggaatcac aatatcgctg tctggcagag taaagttgcg aattaacacg
      181 aaccccttat ccaacaatcg gtctaattgg gcaatcacca ctttttgggc ttccaattcc
      241 ccagcccagc tcaagccgta tttcaacgta ccctgaacac gagcaatggg attgatattc
      301 ccttgtgcgt cttgcaatgg agttctgtcg tataccttca ttcgatctgc ctaaaagagt
      361 ggaacgttct ccagctcatc ctttgtaacg ttttctttgg tcgcaatata ttcgcgatca
      421 tcagctttca ccagcaagat gggcttggaa agcgtaaacg tacttgccag taaatcttgc
      481 cgtgtgccag caacgaactg ttgccctttt tggaggcgtt ccaacagcgt tttacggtcg
      541 agtaaaaccc ggtctgaaaa tgtaaagccg cggcgttcat atccgcgtac tgacacgatc
      601 tgaccattct tataacgtac agcttcaatc acaccatcga ctttttttgc cattcaaaac
      661 ctcttcgctg attttagcac agcttaaatt aagagagaca cgtatcacac aactgtaacg
      721 tctgtgtctc cgtgcctcca gttcttggaa agtcggggcg acaggattcg aacctgcgac
      781 ctcttgctcc caaagcaagc gcgctagccg gactgcgcca cgccccgatg acttctaagc
      841 ctgccgagta taatgcgaag gatgaatacc cgtcaagcaa ttctcaaggc agccttctct
      901 gcgcttcttc tcggactttc agcctgctct acccctcaaa cagtttcccc cactgtaaca
      961 gtgaccgtcc caccgcctac agccacagct acacttacgc cgacacctgt ccctccaacc
     1021 gccaccccaa ctccattagg gtgccttact caacccggca aagtagagca aggtgtgcta
     1081 accgcgacag acccagaaca accctacata atctatttac cgccctgcta ttccgaactg
     1141 accgaccaac gttaccccgt tctttatctt cttcacgggc agacctacac catggatcag
     1201 tgggtgaggc ttggtgttcc gcaagctatg gacgatatgt tcttctctgg tgcaagcgtt
     1261 ccatttattg ttgttttccc tgatgatagc ttgtggaatc taagtgcagg ctggcgcttc
     1321 ggagaacggc tgatcggttc tctcatccca tatattgatg ccacctatcg caccattcca
     1381 gaccgtgatc atcgtgccat tggtggactt tctcgcggcg gcggttgggc agccgagctt
     1441 gggcttcaga atcccggcat gttcatttcg attgggttac attccccggc aattttcaaa
//
LOCUS       DAOREF010000839         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_79845_length_1499_cov_5.745845, whole genome shotgun sequence.
ACCESSION   DAOREF010000839 DAOREF010000000
VERSION     DAOREF010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79845_length_1499_cov_5.745845"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1499
                     /locus_tag="PKI33_14810"
     CDS             <1..>1499
                     /locus_tag="PKI33_14810"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNM38337.1"
                     /translation="TISAGGVTNVYEDDAGHFWICTSDGGLNLMDRKSGTFKHFQHDP
                     ANPYSLSDNNCRQILQDHMGLYWVTTGRGLNLFDPETGRFSRYHKDSSNPYSLSDDNL
                     SAIYEDQGGVLWFGTYGGLNKLNLSTVQFEHYRHIPENPDSLSTSYIYSIYEDKDGIL
                     WVGGDDGVLNRFDRHNNHVIHYEPNADDPNALNKSWSVSALYEDHTGTFWVGTFSGGL
                     HTFDRKTGKFQRYLHNPDDPDSISSDIILAICEDSNGNIWVGTEGGGLNRFDRATATF
                     QHYLPTSNDPKDKRPSSVRGIYEDKAGFLWLTSWYEGLTLFDPRTEQFVNYRHDSENI
                     ESLGNDLTYVVHEDQMGTLWVGTDSGLDRFDRITNTFYHYTVQHGLPNNVIYAIMEDT
                     SGNLWVSTNRGISKFDLRTITFHNYNVFDGLQSDEFNQNAYFQSSSGEMFFGGVNGLN
                     AFYPDQVTNDPYRPPVILTDLQLFNRSVSISEKSVLTKPIWDTEQVTLN"
BASE COUNT          453 a          332 c          320 g          394 t
ORIGIN      
        1 acaatcagtg caggcggagt gaccaatgta tacgaagatg atgcggggca tttctggatt
       61 tgcaccagcg atggtgggtt gaacctaatg gatcgcaaaa gtggcacatt taaacacttt
      121 caacatgacc ctgccaaccc atatagtctt agtgataata actgtcgtca aattttacag
      181 gaccatatgg ggttatattg ggtcactacg ggacgcgggc taaatctatt tgatccagag
      241 accggtagat tcagccgcta tcacaaagat tcaagcaatc cgtattcttt gtctgacgat
      301 aatctttctg ccatttatga agaccaaggc ggcgtgctct ggtttggaac ctatggcggg
      361 ctcaataaac tcaacctcag tactgttcaa tttgagcatt acaggcatat tccagagaac
      421 ccagatagtt taagcacaag ctatatctat tcgatttacg aagataaaga tggtatcctc
      481 tgggtaggtg gagatgacgg tgtattaaac cgttttgacc gacacaacaa ccatgttatc
      541 cattatgaac cgaatgcaga tgatcccaat gcattaaaca aaagctggtc tgttagtgca
      601 ctttacgagg atcataccgg cacattttgg gtagggacat tctctggagg gttacacaca
      661 tttgaccgta aaacaggaaa atttcaacgt taccttcaca acccagatga tcccgatagc
      721 atcagcagtg acatcatcct tgcaatttgc gaagatagca atggcaacat ctgggtcgga
      781 acagaagggg gcggattaaa tcgattcgac cgtgcaactg ctacttttca acattaccta
      841 cccacttcaa atgacccaaa ggacaaacgt cccagcagtg tgagaggcat atatgaagat
      901 aaggcaggtt tcttatggct aaccagttgg tatgaaggac tgactctatt tgatccgcgc
      961 acggaacagt ttgtaaatta tcgacatgac tcagaaaaca tagaaagcct gggcaatgac
     1021 ctaacctatg ttgtccatga agaccagatg ggcacactat gggtaggcac agacagtgga
     1081 ctggatcgtt ttgaccgtat aacgaacact ttctatcatt acactgtgca acacggacta
     1141 ccaaacaatg taatttatgc cattatggaa gatacttctg gtaatttatg ggtttctacc
     1201 aatcgcggta tctctaaatt tgacctacgt acaatcacct ttcataacta caacgtcttt
     1261 gatggactgc aaagtgatga atttaatcaa aacgcctact tccaaagttc cagcggcgag
     1321 atgttctttg gcggtgtgaa tgggctaaat gcattctatc ccgatcaagt cacaaatgat
     1381 ccgtatcgtc cgccagtgat tctgaccgac ttgcaactct ttaaccgctc cgtctccatt
     1441 agcgaaaaat cagtcctcac aaagccaatt tgggatacag aacaagttac tctaaacta
//
LOCUS       DAOREF010000840         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_79930_length_1498_cov_4.068607, whole genome shotgun sequence.
ACCESSION   DAOREF010000840 DAOREF010000000
VERSION     DAOREF010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79930_length_1498_cov_4.068607"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKI33_14815"
     CDS             <1..242
                     /locus_tag="PKI33_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38338.1"
                     /translation="WWKMGSRALLYTSFNVILSASLGISALVPLFIFIPFLVQWLETI
                     WGITHPSVGWKPIQIGMRQLVVSVLWTALFIVFWR"
     gene            245..478
                     /locus_tag="PKI33_14820"
     CDS             245..478
                     /locus_tag="PKI33_14820"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNM38339.1"
                     /translation="MDELKYDLLTELTNRMQADLLESFLEANGIDVELFQESVGHSIY
                     PVTIDLLGRVQVFVPKDKIEQAKILLEEFNAEE"
     gene            482..826
                     /locus_tag="PKI33_14825"
     CDS             482..826
                     /locus_tag="PKI33_14825"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNM38340.1"
                     /translation="MKNTVLLCTKFGMGQTDIASLNQKLISKYLSLLLEGNQLPAAIC
                     FYTDGVKLVTGISPVLEQLSQLEQKGVRLIICSTCLDEMGISRDDVKVGIVGGMPDII
                     EAQTKADKVITL"
     gene            840..>1498
                     /locus_tag="PKI33_14830"
     CDS             840..>1498
                     /locus_tag="PKI33_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNM38341.1"
                     /translation="MNFDLTEEQKTWKKIVHDFAEKELRPKAREVDEKEEFNWEAFRK
                     AGPVGLASMSIPEEYGGSNVDMISNAIAFEELGWGCGSTALAFTAHNGLGTAPIALYG
                     SEELKKKWLPLVGNGKNKLGALALTEPGAGSDIQGGVTTRAEKDGDHWVINGAKMWNT
                     NASIAEYIITLVRTDPKGGSKSLSMILVPTDSKGLSIAPAEKKMGLRGSPTHAVTFDN
                     VR"
BASE COUNT          426 a          307 c          371 g          394 t
ORIGIN      
        1 catggtggaa gatgggtagc cgcgcattgt tgtacacttc tttcaatgtg attctctcgg
       61 cgagcttggg aatatccgct cttgtaccat tgttcatctt catcccattc ttggtgcaat
      121 ggctggagac gatctgggga atcacccatc catctgtggg ttggaagcct atccaaattg
      181 gtatgcgcca attagttgtg agcgttctat ggactgcatt atttatagtg ttctggaggt
      241 aaaaatggac gagctaaaat atgacctatt aactgaactc acaaatcgca tgcaagcaga
      301 tttactggaa agttttctcg aagcgaatgg cattgatgtg gagttatttc aagaatctgt
      361 ggggcactcc atctatccgg tcacaatcga cttattaggg cgcgtgcagg tttttgtacc
      421 caaagacaaa atagagcagg caaaaatact gcttgaagaa ttcaatgctg aggaataaga
      481 aatgaaaaac accgttctac tttgtacaaa atttggaatg gggcaaacgg atattgcctc
      541 tctaaatcag aaattgatta gcaagtattt gagcctgtta ctggaaggga atcaacttcc
      601 tgctgccatt tgtttttata cagatggggt taagctggta accggtattt ctccggtact
      661 ggagcaactt tctcaattgg aacaaaaagg ggttcgcctt attatttgct ctacctgtct
      721 tgatgaaatg ggaatttctc gtgacgatgt aaaagtaggc attgtaggcg ggatgcccga
      781 tataattgaa gcacaaacaa aagcggataa agtcattacg ctataaatta ggagttatta
      841 tgaatttcga tctcaccgaa gaacaaaaaa cgtggaaaaa gatcgtccat gactttgctg
      901 aaaaggagtt acgccccaaa gcacgtgaag tggatgaaaa agaagaattt aattgggaag
      961 cgttccgcaa ggctgggcct gttggtttgg caagcatgag tattcccgaa gaatatggcg
     1021 gttccaatgt agacatgatc tcaaatgcca ttgcctttga agaattaggc tggggttgtg
     1081 gctcaacggc gcttgctttc acggctcaca atggcttagg gacagctcct attgctttgt
     1141 atggtagtga agaactaaag aaaaaatggc tgcctttggt aggcaacgga aagaataaac
     1201 ttggcgcctt agctctcacc gaacctggtg ctggttctga tattcaaggt ggggtcacca
     1261 ctcgcgccga gaaagatggc gatcactggg tgattaatgg cgcaaagatg tggaacacca
     1321 atgccagtat cgccgaatac atcatcaccc tcgtccgcac cgacccgaaa ggcggttcaa
     1381 aatcgttgag catgatcctc gtgccaaccg actctaaagg cttgagcatt gcacctgccg
     1441 aaaagaaaat gggcttgcgc ggctccccca cccatgcagt gaccttcgac aacgtgcg
//
LOCUS       DAOREF010000841         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_80046_length_1497_cov_4.103329, whole genome shotgun sequence.
ACCESSION   DAOREF010000841 DAOREF010000000
VERSION     DAOREF010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80046_length_1497_cov_4.103329"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKI33_14835"
     CDS             <1..409
                     /locus_tag="PKI33_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNM38342.1"
                     /translation="SATTVAMDFGRITVDEDLVLYLFGTPGQKRFDFMWEILSEGMLG
                     FIVMVDSTRPETFREARSILETFRAYAPTPFVVAANKQDVDDAWDLEDVRHALRLDGD
                     TKLLPCVATNRDTVKTVLLELLYSILKEMDSEK"
     gene            430..1227
                     /locus_tag="PKI33_14840"
     CDS             430..1227
                     /locus_tag="PKI33_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM38343.1"
                     /translation="MSSITTDQGIVHYEVYGRGRPVILLHGYQGSWGLWQQTMGYLGA
                     FYRTYALDFWGFGESGTKRETYAVKDFVGLVNQFMEQLGIIRAPLVGHSMGGTVSLLT
                     AMQYPDRVEKVVVIGSPISGSSLYFFPRVFGYRAFGWLTYHNLWIYKSFYRILAPYYS
                     KDKNWAAMMDRDVSRTTLEAFFASIGSLRRTDLRPSLHTIQVPVMGMYGDNDIVVNPN
                     QWKPLQEGVAKSHIERFPKAGHFIMLDEPLEFTQKLKAFLDQENPAS"
     gene            1224..>1497
                     /locus_tag="PKI33_14845"
     CDS             1224..>1497
                     /locus_tag="PKI33_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNM38344.1"
                     /translation="MSPQKYYPQKMGRIILQGMEEVMGVNGVETTLHLGSLDHIIQNR
                     PTATSDRTFPFEVVSAILASLEMVYGPHGGRGVALRVGRSTFRYGLK"
BASE COUNT          364 a          325 c          387 g          421 t
ORIGIN      
        1 gtctgctact acagtggcga tggatttcgg gcgtatcact gtggacgaag acttggtcct
       61 gtacttgttt ggtacacccg ggcaaaaacg cttcgatttt atgtgggaga ttctttcaga
      121 aggaatgctg ggtttcatcg ttatggtgga tagcacccgc cctgaaactt tccgtgaggc
      181 tcgctcgatt ctggaaacct tccgcgcgta cgccccgacc ccttttgtcg ttgctgccaa
      241 taagcaggat gtggacgatg cctgggatct tgaggatgtg cgccatgctt tacgccttga
      301 tggcgataca aagttactac cctgtgtggc aacgaaccgc gataccgtca aaacggtttt
      361 gctggagttg ttatatagca tcttaaaaga aatggactcc gaaaaatagg gtcaggttgt
      421 atccttgctg tgtcttcaat tactactgat cagggaatag ttcattatga agtatacggg
      481 cgggggcgcc cggtaatttt gctgcatggc tatcaaggct catggggatt gtggcagcaa
      541 actatggggt atttgggtgc tttttatcgc acttatgcct tggatttttg gggatttggc
      601 gaatctggta caaaacgtga gacatacgct gtaaaagatt ttgttggctt ggtcaaccaa
      661 ttcatggagc aattgggaat tatccgggct ccattggttg ggcatagcat gggtggtact
      721 gtcagtttat tgacggccat gcaatacccg gaccgggttg aaaaggttgt ggtaattggc
      781 tcacctattt caggttcctc tttgtatttt ttcccgcgcg tgttcggata tcgtgcattt
      841 ggctggctga cctaccataa tctttggatc tacaagtctt tttaccgcat ccttgcacca
      901 tattattcca aggataagaa ttgggctgcc atgatggacc gggatgtatc tcgaacaacg
      961 cttgaagcct tttttgcaag cattggctcg ctcagaagaa cggatttacg ccccagcctc
     1021 cataccattc aagtccctgt aatgggtatg tatggagaca atgatattgt ggtaaaccca
     1081 aatcaatgga agccattgca agaaggtgtt gcaaagtctc atattgagcg tttcccaaaa
     1141 gccgggcatt tcatcatgct ggatgagccg ctagaattca cccaaaaact aaaagcattc
     1201 ctagaccaag agaatcccgc ttcatgagtc cgcagaaata ctatcctcag aaaatgggga
     1261 gaatcatcct gcaaggcatg gaagaagtaa tgggtgtgaa cggggtggaa acaacccttc
     1321 atcttggttc ccttgaccac attatccaaa acaggccaac agctacgtcg gaccgcactt
     1381 ttccttttga ggtggtcagt gccattttag cgtctcttga aatggtttac ggtccacatg
     1441 gtggaagagg agtggcgctg cgtgtaggcc gatccacttt ccgatatgga ttaaagg
//
LOCUS       DAOREF010000842         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_80250_length_1495_cov_4.869444, whole genome shotgun sequence.
ACCESSION   DAOREF010000842 DAOREF010000000
VERSION     DAOREF010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80250_length_1495_cov_4.869444"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKI33_14850"
     CDS             <1..471
                     /locus_tag="PKI33_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38345.1"
                     /translation="HTFQIDVENLKLINAKNLGIKPAYYDHNLGQKMEFPAPGESSQL
                     AQSLISNYGYLYYPLGVESDGNCVEVLESTFNTKIILEASSIIMDGKSTGPYTWTIDY
                     TYLLNTTNNPVPNATFIVNSAIESTDALPTVPVISDAFWEEYAKFIWGKLTPKH"
     gene            575..>1495
                     /locus_tag="PKI33_14855"
     CDS             575..>1495
                     /locus_tag="PKI33_14855"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNM38346.1"
                     /translation="MSTVFTFVPSREHEYPDHPERPGRLDILEPLLPSFGAEQIQAIR
                     ATEADVALVHPVKMIRSLQEACMEGPGVIDYAPTYVTQTSYEDALKAVGGVLTCSHAV
                     MNGGARNAFAIVRPPGHHAEPDRAMGFCLFSNIAIAAQDLLRKGLKRVMVIDYDAHHG
                     NGTQAAFLHEERLGFVSTHQWGIYPGTGWIDDAPHANQRIVNVPLPAYAGDKTFVRIA
                     DEVFAPMVRAFKPEMLLISAGFDSHWNDPITSLGLSTRGFYEVSKKLVEMAEEHCGGK
                     IVFVLEGGYDPRNVANGVGAVFDALTSRPLG"
BASE COUNT          384 a          361 c          363 g          387 t
ORIGIN      
        1 catacgtttc aaattgatgt agaaaatcta aagcttatca atgccaaaaa cctcggtatt
       61 aaacctgcat actatgacca taaccttggg caaaaaatgg aattccccgc gccgggggaa
      121 tcttctcagc tcgcacaatc tttaatatca aactatggat atttgtacta tccattaggc
      181 gtagaatccg atggcaattg tgtggaagtc cttgaatcga ccttcaacac caagatcatc
      241 cttgaagcat cctccattat tatggatgga aagtctacgg gaccatatac atggacgatt
      301 gattacacct atctcttaaa cacaacgaat aaccctgtcc ccaatgcaac ctttatcgtt
      361 aactccgcta ttgagtcgac agatgccttg cccactgttc ccgtcatctc agatgcgttc
      421 tgggaagaat acgcaaaatt tatttggggc aagctcacgc caaaacacta attatggctt
      481 tttttccaca gcaggtttta aaaaatgctc aaatctgctg tgacttctgt ggcttaagtt
      541 tatgaattca ccaacagggg gctataatta aatcatgtct accgtattta catttgtccc
      601 atcgcgggaa catgaatatc cagaccaccc cgaacgccca ggcaggttgg atattttgga
      661 gcctttgcta ccctctttcg gcgcggaaca gatccaagcg attcgggcaa ccgaagcgga
      721 tgtggctctc gtccacccgg tgaagatgat tcggtctctg caagaagcct gcatggaagg
      781 acccggtgtc attgattatg cgcctaccta tgtaacgcag acttcttatg aagatgcgct
      841 gaaagccgtt ggcggtgtgt tgacctgttc tcatgcggta atgaatggag gtgctcgtaa
      901 tgcatttgcc atcgtccgcc cgccggggca tcatgccgag ccagaccgcg cgatggggtt
      961 ttgtctcttc agcaatatcg ccatcgctgc acaagatttg ctgagaaaag gcttgaagcg
     1021 tgtgatggtg atcgattatg acgcacatca tggcaatggg acacaagcag catttttgca
     1081 cgaagaacgg ctgggatttg tctccacgca tcaatgggga atttaccccg gcaccggctg
     1141 gatagacgat gcgcctcacg ccaaccaacg cattgtcaat gtgccgttgc cggcttatgc
     1201 aggtgacaag acctttgtgc gcatcgcaga cgaagtcttt gcgcccatgg tgcgtgcctt
     1261 caagcccgag atgctattga tctcagcagg gtttgactcg cattggaatg accccatcac
     1321 ttcattggga ttgtcgacac gtggctttta tgaagtgtcg aaaaaattgg tggaaatggc
     1381 agaagaacat tgcgggggaa aaatcgtttt tgtattggaa ggtgggtatg atccgcgaaa
     1441 cgtcgcgaac ggcgtaggcg cagttttcga tgcgctgacc agccgtccct taggg
//
LOCUS       DAOREF010000843         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_80341_length_1494_cov_3.559416, whole genome shotgun sequence.
ACCESSION   DAOREF010000843 DAOREF010000000
VERSION     DAOREF010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80341_length_1494_cov_3.559416"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKI33_14860"
     CDS             complement(<1..506)
                     /locus_tag="PKI33_14860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNM38347.1"
                     /translation="MPTLHWNGKHLSQPQPASLTLDSVLHPKGRGYPKSRTDGRVIQG
                     DNLAVMAALLPEYEGRINLIYADPPFFTNRQFHARVGRGEDSRKPSKWKLAEGYQDSW
                     TDMDSYLQFLYERLSLMHRLLAKNGTLYLHLDWHADSHARLILDEIFGAENFINEIIW
                     AYHGPSPIR"
     gene            complement(565..>1494)
                     /locus_tag="PKI33_14865"
     CDS             complement(565..>1494)
                     /locus_tag="PKI33_14865"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNM38348.1"
                     /translation="PTDKSRIELSHYRDELVHVYGEITAEEPKHNPVWYRVWGGYLHR
                     GRLHRVRTIYQTPIDSIPEGTRLIADVSVPFTTPYRYSKTYGWQQMSPPLYYGSVHFI
                     EAVEQGPEMADYKGPWYRIFDELDSNVTYYAPAIHMRILPQDMLTPISPDVPYEDKLI
                     EINLTSQMLYAYEYGSLVFQTNISSGVPGDPTGGTGVPTTTPTGKFTILDKVPAKHMG
                     YSYFGAQTTGNVLADVDNYVLPGVPWTSFFTSQGHAFHGTYWHENFGSPMSHGCINMR
                     TNEANWIFRWARPEPIVGNVATRGLGTTVEIHY"
BASE COUNT          388 a          335 c          413 g          358 t
ORIGIN      
        1 cgtatcggag aaggtccatg atatgcccag ataatttcat tgatgaagtt ctcagcgcca
       61 aaaatttcgt cgaggatgag gcgggcatgg gaatcagcgt gccagtcaag gtgaagatat
      121 aacgtgccgt ttttggcgag cagtcgatgc atgagactga gacgttcgta cagaaattgc
      181 aggtacgagt ccatatctgt ccatgaatct tgataacctt cagcaagttt ccacttggaa
      241 ggtttgcgag agtcttctcc cctccccaca cgggcatgga attgtctgtt agtgaaaaat
      301 ggtgggtcgg cgtaaatgag gttgatgcgc ccttcatatt caggcaacaa agcagccatc
      361 acagccaaat tatccccttg aatgactcgt ccatcggtac gcgatttggg gtagccacgt
      421 cctttcgggt ggaggacaga gtcaagggtt aatgaagcag gttgcggctg ggaaagatgc
      481 ttcccgttcc agtgtaaggt aggcatgaga gcagggaaac ctcaaatggt ggattcggtt
      541 tgggagaaga aatcccaaac cgttttagta atgaatttca acagtagtgc caagcccgcg
      601 agtggcgaca ttgcccacaa tcggttctgg gcgtgcccag cggaagatcc aattggcttc
      661 gttggtgcgc atgttgatac agccatggct cataggcgag ccaaaatttt catgccaata
      721 ggtgccatgg aaggcgtgac cttgcgaagt gaagaatgaa gtccacggaa cgccggggag
      781 gacgtagtta tctacatcgg caaggacgtt acccgttgtt tgcgcgccaa agtaactata
      841 ccccatatgc ttggcaggca ccttatccaa aattgtaaat ttgccagtcg gtgttgtagt
      901 gggcacacct gtgccgcccg tagggtcgcc aggaacaccc gaagagatat tggtttggaa
      961 gaccaaactg ccgtattcat aagcatataa catttgcgat gtgaggttga tctcaatcaa
     1021 tttatcttcg tatggcacat caggagaaat aggagtcaac atatcctgcg ggaggatgcg
     1081 catatgaatg gcaggtgcat aataggtgac gtttgagtcg agttcgtcga aaatgcgata
     1141 ccaaggacct ttgtaatctg ccatctcggg tccctgttct acggcttcaa taaagtgaac
     1201 cgagccataa tacaacggcg gagacatttg ctgccaacca taggtcttgg aatagcgata
     1261 gggtgtggta aatggcacag aaacatctgc gatcagacgt gtaccttcag ggatggagtc
     1321 tatcggggtc tggtagatcg tgcgcacgcg gtgcaaacgt ccgcggtgga ggtagccgcc
     1381 ccacacccga taccaaaccg ggttgtgttt cggttcttca gcagtgatct cgccatacac
     1441 atgcaccaac tcatcgcgat aatgagaaag ctcaatccgg cttttatcgg ttgg
//
LOCUS       DAOREF010000844         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_80442_length_1493_cov_4.662726, whole genome shotgun sequence.
ACCESSION   DAOREF010000844 DAOREF010000000
VERSION     DAOREF010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80442_length_1493_cov_4.662726"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKI33_14870"
     CDS             <1..609
                     /locus_tag="PKI33_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38349.1"
                     /translation="KALTSVYADEVSLDIRELEHIRLNFAFRIQPEGSQIATTSPLRM
                     GPILRDVPLHVLLEFVVSPEALKGEKVLVLDGLLKASIASNPIPASPIRLKLTRPLEQ
                     SPSAEPPPTRVLKALSRLTLYRMQERAQSAAQNGQFDVAARHLQNLATHLLSQGEHAV
                     AKTALLEAQSLENTRTWSESGSKDIKYKTRALLLDSGKEKKS"
     gene            606..1112
                     /locus_tag="PKI33_14875"
     CDS             606..1112
                     /locus_tag="PKI33_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNM38350.1"
                     /translation="MIICSNCKHANMTGAMFCVECGAQLIGRDTLTTQNIATDSLGRA
                     SKRKTGELSQPIEGVDAWANLQLLDTGQVLPLSSRNEFTLGRISEGQPIMPDIDLSPY
                     QAYAAGVSRLHAVIKRSGSYVKFMDLGSANGTFLNGIRLSPNVEQSIQHGDIVSLGKM
                     RIQILIEK"
     gene            1134..1376
                     /locus_tag="PKI33_14880"
     CDS             1134..1376
                     /locus_tag="PKI33_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38351.1"
                     /translation="MAYTVILHIANEASVAGEVDELPKPTDTIIVVANPRQKDGKDLH
                     YIDQGTVKVIWPLAKVSFVEILGDSEEEKIIGFVRE"
BASE COUNT          419 a          307 c          376 g          391 t
ORIGIN      
        1 aaagcgttga ccagtgttta cgctgatgaa gtgtcgctgg atattagaga acttgagcac
       61 attcgcctta actttgcatt tcgcatccag ccggagggca gccaaattgc aaccacttcg
      121 ccgttgcgaa tgggaccaat cttgcgtgat gtgcctttgc atgtcttgtt ggaatttgtt
      181 gtcagccccg aggcattgaa aggggagaag gtcttagtat tggatggact cctaaaagca
      241 tccattgctt ccaaccctat tcctgcatct cccatccgcc tcaaactgac ccgtcccttg
      301 gagcaaagtc cctcagcaga gccaccgccg acgcgggttt tgaaggcgct ttcccgcctt
      361 actctgtatc gcatgcaaga acgggcgcaa tctgctgccc agaatggaca gtttgatgta
      421 gcagccaggc atttgcaaaa tttagcaacg catttgcttt cacaaggcga acatgctgtt
      481 gcaaaaactg cattgcttga agcacaaagt cttgaaaata cgcgtacctg gagcgaatct
      541 gggagcaagg atataaaata taaaacacga gcgctgttat tggacagtgg caaggagaaa
      601 aaatcatgat aatctgttcc aattgcaaac atgcaaatat gacaggtgcc atgttctgtg
      661 tggaatgtgg cgcccagttg atcggaagag acacactaac cacccagaat attgcgacag
      721 atagccttgg aagagcaagc aaacgaaaaa ccggtgaatt gagccagcca atagaaggtg
      781 ttgatgcttg ggcgaatcta caacttttag atactgggca agtgttacct ctctcttctc
      841 gaaatgaatt cacgttaggg agaattagtg aggggcaacc aattatgccg gatattgacc
      901 tctctcctta tcaggcatat gctgctgggg tttcacgctt acatgctgtc atcaaaagaa
      961 gcggctctta tgtcaaattt atggacttgg gttctgcaaa cggaaccttt ctaaatggca
     1021 ttcgtttatc accaaatgtg gagcaatcga ttcagcacgg tgatattgtg tcattgggta
     1081 agatgagaat tcaaatatta attgagaaat agaacgaagg agagacaacg gatatggctt
     1141 acaccgttat tttgcatatt gctaatgaag catctgtggc tggtgaagtg gatgagcttc
     1201 ctaaaccaac cgatacgatt attgttgttg ccaatccccg ccagaaagat ggcaaagact
     1261 tgcactatat cgatcaagga accgttaagg tgatttggcc tttggcgaaa gtaagttttg
     1321 tcgaaatcct tggtgactct gaagaagaga agatcattgg ttttgtgagg gaatagacgt
     1381 atgacagaaa tggatcgacg tcgcatcctg gttgtggatg atgaagagcg tatggtgcgc
     1441 tttattcgta tgaacctgga gcatgatggt tttcaggtag gggaggcgtt taa
//
LOCUS       DAOREF010000845         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_80461_length_1493_cov_2.787204, whole genome shotgun sequence.
ACCESSION   DAOREF010000845 DAOREF010000000
VERSION     DAOREF010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80461_length_1493_cov_2.787204"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKI33_14885"
     CDS             <1..363
                     /locus_tag="PKI33_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNM38352.1"
                     /translation="STGGLHAAALFDLEGNLLAVREDVGRHNALDKLIGWGLLNDKLP
                     FHDKVLMVSGRASYELLQKSFSAGISVFCAVSAPSSLAVEFAERFGITLIGFLRGQRF
                     NVYANVQRMQTPEPVFSN"
     gene            450..893
                     /locus_tag="PKI33_14890"
     CDS             450..893
                     /locus_tag="PKI33_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM38353.1"
                     /translation="MESEITISLASIHDAPDIAKLNRLFNEVDESPEAIASRMSDPNC
                     VETVVLAKVADKALGFALVRVVSSVLYSTPHAELTELYVLEEYRQRGIASELITFAEQ
                     IAVQKGTRIILVQTGDDNSPALSLYRKHGYEEYDLTLRKIMVESK"
     regulatory      951..1046
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1297..>1493)
                     /locus_tag="PKI33_14895"
     CDS             complement(1297..>1493)
                     /locus_tag="PKI33_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38354.1"
                     /translation="FKCRLIYDKPFTSFSSQNTFRFIPFYIYRTREQTVCYSISRNNK
                     MDTKYHPDKVYYLAEDEKYE"
BASE COUNT          395 a          308 c          347 g          443 t
ORIGIN      
        1 tccacaggtg gacttcacgc cgcggcatta ttcgacctcg aaggcaatct acttgctgtg
       61 cgcgaagatg tgggacgaca taacgctttg gataaattga tcggctgggg tctgctcaat
      121 gacaagcttc catttcacga taaagtctta atggtcagcg gacgtgcaag ttatgagctc
      181 ttgcaaaaaa gtttttcggc gggcatctcg gtcttctgcg ccgtctctgc gccgagtagt
      241 ttagccgtag agtttgccga gcgctttggc atcaccctca tcggcttctt gcgcggacaa
      301 cgcttcaacg tctatgccaa tgtgcagcgc atgcaaacgc cagaacctgt attttcaaat
      361 tgacaattcc acttgcgttt gatataaaac attcgagata cttcagcctc tcggtaagag
      421 aggctgttct tttaatatag gatatttaaa tggaatcaga aataaccatt tctcttgcat
      481 ccatccacga tgcgcctgac attgccaagt tgaatcgact cttcaacgaa gtggacgaat
      541 caccagaagc cattgcgtct cgtatgagcg acccaaactg tgtagagacg gtcgtccttg
      601 caaaagttgc ggataaagca ctgggttttg cccttgtgcg cgtggtgtca agtgttttat
      661 attccacccc tcacgccgag ttgaccgagt tgtacgtgct ggaagaatat cgacaacgtg
      721 gtattgccag tgaattgatt accttcgcgg aacaaatcgc agttcaaaaa ggtacgcgga
      781 tcatccttgt gcagacgggt gatgataata gccctgcttt gtctttatat cggaaacatg
      841 gatatgaaga atatgactta accttgagaa aaattatggt cgaatcaaag tgatttttta
      901 tttgacatcc ccttctcagc atgttataaa aacgtcacaa gttacggata gactcatcca
      961 gagaagctga gggaccgacc ctgtgacgct tcggcaaccg ttcaagcggt gccaacttcg
     1021 gcagacgact gtctgggaga tgagagctga tatggtttca tgtcgcctct cattgcgaga
     1081 ggcgtttttg ttgtaggttt aaacccgctt gaaataaaat tcctgttttt agtagtgtga
     1141 aaatccaaac atataggaaa atctatgagg aagtattatc ttagaaaacg taatattatt
     1201 tttctagctt gtttattagc gttgccagtg ctattctcta ttttaatttt tgatatcctc
     1261 gtttctgtaa ataagatgcc gggttggata gtcgctttat tcgtatttct catcttcggc
     1321 gagataatac actttgtccg gatgatattt agtgtccatc ttgttgtttc ggctaatgga
     1381 ataacataca gtttgttcac gcgttcggta aatgtaaaat gggatgaagc ggaaggtgtt
     1441 ttgcgaacta aaagaagtga agggcttatc gtaaataagc ctgcatttga aaa
//
LOCUS       DAOREF010000846         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_80553_length_1492_cov_2.990257, whole genome shotgun sequence.
ACCESSION   DAOREF010000846 DAOREF010000000
VERSION     DAOREF010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80553_length_1492_cov_2.990257"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKI33_14900"
     CDS             complement(<1..405)
                     /locus_tag="PKI33_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38355.1"
                     /translation="MKKISQHPILILLVINLAIGLMTFRSYGLAWDEPLFYDYGQALK
                     YAYTPANWFSGNFDVTQSFGSSGSDHANRGPAYLLVAMPLVSFLEWLGLDLASAWHLV
                     NFLTFNFGIYFLYRLASKWMNKESAFAVAALFA"
     gene            complement(411..872)
                     /locus_tag="PKI33_14905"
     CDS             complement(411..872)
                     /locus_tag="PKI33_14905"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNM38356.1"
                     /translation="MSKAKTSHASRVYLSQLMQPEHANHHGNVHGGWIMKLVDEAGAL
                     SCMRHAQMRVVTVAVDSLVFREPIKIGDLVTFTAEVTYTGRTSMEAEVQVTAEDPITG
                     ERTHTNTAYLVYVGLDDNNRPAPIPQLVAETEEEKARLELAQQRQKRRIQK"
     gene            complement(875..1249)
                     /locus_tag="PKI33_14910"
     CDS             complement(875..1249)
                     /locus_tag="PKI33_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38357.1"
                     /translation="MAQVSFIPIYSTKGDADAFLLYPYLFNRTGDWIGFVTPARDVYS
                     VLGEYVGTLTNDPRIVAKRAVDETKPRVAPPPAPKRVQLPANVPLAPMMPELSHSLVD
                     VLIDMPEKLHTIDSGESRQDMD"
     gene            complement(1261..>1492)
                     /locus_tag="PKI33_14915"
     CDS             complement(1261..>1492)
                     /locus_tag="PKI33_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38358.1"
                     /translation="GMFFAVNTAGMLIRIPILHYLEPPLLQFVEDSFHTTYLTAEFYA
                     KNLTLATAVGIVMLWNYFVNRYWTYNDIDDDN"
BASE COUNT          389 a          319 c          407 g          377 t
ORIGIN      
        1 ggcgaaaagc gccgcaactg caaaagcaga ttctttgttc atccattttg aggcgaggcg
       61 gtagaggaag taaatgccaa agttgaaagt aaggaagtta accaagtgcc aagctgaggc
      121 gaggtcgagt ccaagccatt ctaaaaaaga gacgagcggc atggctacca aaagataagc
      181 gggtccgcga ttggcatggt cgctgccgct cgagccaaac gattgggtga catcaaaatt
      241 tccgctaaac caatttgcgg gggtgtaggc gtatttgagc gcttgaccgt aatcgtagaa
      301 gagcggctca tcccaagcca gtccataaga acgaaacgtc atcaatccaa tagcaagatt
      361 gatgacgagc aatatcaaaa taggatgttg acttattttt ttcaagagga ttacttttga
      421 atgcgtctct tttgacgttg ttgtgccaat tcgaggcgtg ctttttcttc ttcagtttct
      481 gcgaccaatt gcgggatggg cgcagggcgg ttgttgtcat ccaaaccgac atagacgagg
      541 taggcagtat tggtatgagt gcgttcgcct gtgatggggt cttctgcggt cacttgtact
      601 tcggcttcca tggaggtgcg cccggtgtag gtcacttcgg cggtgaaggt gacgaggtct
      661 ccgattttga tgggttcgcg gaaaacgagc gagtcaacgg cgacggtgac aacgcgcatt
      721 tgggcgtgac gcatgcaaga aagtgcaccg gcttcatcaa ctagtttcat gatccaaccg
      781 ccatggacat tgccatgatg gttggcatgt tcgggttgca taagttgtga gagatacaca
      841 cgcgaagcat gggatgtttt tgcttttgac atgactaatc catatcctga cgggactcgc
      901 cactatcaat tgtgtggagt ttttccggca tgtctataag aacatccacg agggagtgac
      961 tgagttctgg catcataggt gccaacggaa cgtttgcagg cagttgaact cttttgggcg
     1021 cgggcggagg ggcaacacga ggtttggttt catcgacagc gcgtttcgct acgatgcgcg
     1081 gatcattcgt cagtgtgcca acgtattcac ccaataccga atatacatcc cgagctggtg
     1141 tgacgaagcc gatccaatct cctgttcgat taaagagata aggatataaa aggaacgcgt
     1201 ctgcgtcccc tttagtgctg tagattggaa taaatgatac ttgtgccata acgtcctgcc
     1261 ttaattgtcg tcgtcaatat cattatacgt ccagtaccgg ttgacgaaat agttccataa
     1321 catcacaatg cctactgctg tggcaagagt caaattcttg gcataaaact cggctgtaag
     1381 ataggtcgtg tgaaaagagt cttcgacaaa ttgcaataac ggtggttcga gataatgaag
     1441 gatgggaata cgaatcagca tgccagctgt gttgaccgca aagaacattc cc
//
LOCUS       DAOREF010000847         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_80758_length_1490_cov_3.983972, whole genome shotgun sequence.
ACCESSION   DAOREF010000847 DAOREF010000000
VERSION     DAOREF010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80758_length_1490_cov_3.983972"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKI33_14920"
     CDS             complement(<1..766)
                     /locus_tag="PKI33_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNM38359.1"
                     /translation="MANKKKSPTIYDVAELSGVSISTISRVLNSPDKVNTETRQQVMS
                     AIDRLGFVPKAEARARAMQNTNRIGVITPFFTAPSFVQRLRGVASRLSRENYELVIYP
                     VDSEEQLQGYLSSIPVMRNLDGLIIMSLAVESSDVERLISNKMETVLIEFSNDQLNSI
                     VIDDFQGGRLVAEHLIQKGYKSFGFLGDIEPPERNIIHPVRSRLGGFQAVLKDAGLQL
                     PAKNIKQAFYAHESSREGAYELLTMPNRPAAIFAASD"
     gene            940..>1490
                     /locus_tag="PKI33_14925"
     CDS             940..>1490
                     /locus_tag="PKI33_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNM38360.1"
                     /translation="MRTKKRAVTIQDVAKAAGVSVSTVSRVLNGKTDVAGETQERILK
                     IINKLGFTSNLAARSMRSLKKNLIGLVVPDIHPYSVEIMKGVNKAIAESNYDLLVYTT
                     GDALKHGATLHEQHYISLLSNSITDGVIIVASATAEFTAEAPIVAVDPHVPNPKYPSV
                     QGMNYHGALDAMEYLFKLGHQRIG"
BASE COUNT          413 a          340 c          348 g          389 t
ORIGIN      
        1 tatcagaggc ggcaaaaatg gcagcaggac ggttgggcat ggtgagcaat tcatatgcgc
       61 cttcgcgtga actttcatgg gcataaaaag cttgcttaat atttttcgcc ggtagctgca
      121 agccggcatc tttcaacaca gcttgaaacc cgcccaaacg tgaacgcacg gggtgaatga
      181 tgtttcgttc cggcggttca atatcgccta aaaagccaaa agatttataa cccttttgaa
      241 taagatgctc ggcaaccaaa cgcccacctt gaaaatcgtc gatgacgata ctgttcagtt
      301 ggtcgtttga gaactcgatc agcacggttt ccattttgtt gctgatcagg cgctccacat
      361 cactgctctc tacagccaac gacatgatga tcagcccatc taaatttcgc ataacaggaa
      421 tagaagagag atagccctgc aattgctctt ccgaatcgac cgggtaaatg accagctcat
      481 aattctcgcg cgaaagtctg cttgccacgc cgcgcaagcg ttggacgaag gatggggcgg
      541 taaaaaatgg ggtgatgacc ccgatgcggt tggtgttctg catggcacgc gcacgcgcct
      601 cagccttcgg gacgaagccc agccggtcaa tagcagacat aacctgttga cgggtttcgg
      661 tattaacttt atctggtgag tttagcaccc gcgaaatggt ggaaatgctt acgccagaaa
      721 gttctgcaac atcataaata gtaggggatt ttttcttatt tgccatattg tatgtgaaag
      781 ctaagtgaaa gcactttcag tttaacatga aacagtctca aattccagat tcttagagtt
      841 tcaattaagt agttgacttt ttattttttt tatgcaataa taccggtaac gtttccggta
      901 ttgataccga aaaatagagc attttcacca ggaattggta tgcgaactaa aaaacgcgca
      961 gtgacaattc aagatgttgc aaaagcagca ggtgtttctg tatcaaccgt ttcgcgtgta
     1021 ttaaacggta aaacggacgt tgcgggggaa acacaagaac gtattcttaa gattatcaat
     1081 aaactcggtt tcacatccaa tctggcggct cgcagcatgc gcagcctcaa aaagaatttg
     1141 atcgggttgg tcgttcctga tattcacccc tactccgttg agatcatgaa gggtgttaac
     1201 aaggcgatcg cagagtcaaa ttacgacctt ttggtataca ccactggtga tgcgttgaaa
     1261 catggggcaa ccttacacga gcaacactat atttctctgc ttagcaactc cattaccgat
     1321 ggggtaatca ttgtggcatc tgcgactgcc gaattcactg ccgaagctcc cattgtggca
     1381 gtggacccgc atgtgcccaa cccaaagtat ccttcggttc aaggtatgaa ctatcacggc
     1441 gctttggatg ccatggaata tctcttcaaa ttaggacatc aacgaatcgg
//
LOCUS       DAOREF010000848         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_81320_length_1485_cov_3.960839, whole genome shotgun sequence.
ACCESSION   DAOREF010000848 DAOREF010000000
VERSION     DAOREF010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81320_length_1485_cov_3.960839"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKI33_14930"
     CDS             complement(<1..754)
                     /locus_tag="PKI33_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM38361.1"
                     /translation="METRFFNYKTGQIAYEVQGEGPLVVCVPSLGDVRGEYRFVVPEL
                     VKAGYRVATMDVRGHGETSTTWDDFSVAAIGEDILALIRELKAESAIVVGTSMGGGAA
                     IWSAVEAPELIRGMLLIDPFVDGDSNPFFIFLLSIMFARPWGAAMWKKYYSSLYPTRK
                     PADFDLYVETLYKNINQLGRLEAVMAMMRASKRSSGERMPNVKQPALILMGSKDPDFK
                     NPIAEAKRVAEAVRGNYKIIENAGHYPHAEMPE"
     gene            875..1408
                     /locus_tag="PKI33_14935"
     CDS             875..1408
                     /locus_tag="PKI33_14935"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNM38362.1"
                     /translation="MSFKVSVYIATSLDGFIARKNGDLDWLPIPTEGGEDYGYAQFMS
                     TVDVIVMGRNTFEKVISFGGWHYDKKVFVLSSRRLDIPSELNEKVEALHQTPRELIRE
                     LERRGIRHIYLDGGVTIQRFLREGLVNEMIITTIPVLIGEGLPLFGELEKDIQLELLD
                     VSSFSNGLAQKKYKVLA"
BASE COUNT          382 a          351 c          340 g          412 t
ORIGIN      
        1 cttcaggcat ttcagcatgc ggataatgcc cggcattttc gatgatttta taattgccgc
       61 gcactgcttc tgcaacccgt ttcgcttcgg caatcgggtt tttgaaatca gggtctttgc
      121 tgcccatcaa gattaacgca ggttgtttta cgttgggcat acgttcccct gaagaacgtt
      181 tagaagctcg catcattgcc atcactgcct ctaaccgccc tagctgattg atgtttttat
      241 acaatgtctc aacataaagg tcgaaatcag cgggtttgcg cgttggatat aaggacgagt
      301 agtatttctt ccacatcgct gcaccccacg ggcgagcaaa catgattgaa agcaagaaaa
      361 tgaagaaagg attgctatcg ccatccacaa acggatcgat gagaagcatg ccgcgaatta
      421 actctggcgc ctctactgct gaccagatag cggctccccc gcccatggaa gttcccacta
      481 cgatagctga ttctgctttc aactcgcgta tgagtgcaag aatgtcttca ccgatggctg
      541 ccactgagaa atcatcccaa gtggtactcg tctcgccatg cccgcggacg tccatggtag
      601 cgacgcgata gccagccttg accagttctg gtacgacaaa acgatattcg ccgcgcacat
      661 ctcctaaaga cggcacacat accactaatg gaccttctcc ctgcacctca taggcaattt
      721 gcccggtttt ataattgaaa aatcttgttt ccatcgttct ctccatacta ctaattatat
      781 tagtatttta actaaatata ttagtttttg tcaagaggga acttttttgt acgttatggc
      841 gtttattcac ttcaactgtt tatgaggagg aagcatgtct tttaaagttt ctgtgtatat
      901 tgccaccagt ctcgacggtt tcatcgcccg taagaacggg gacctcgact ggctgccaat
      961 ccccaccgaa ggcggcgaag attacggcta cgctcaattc atgtccacgg tggatgtgat
     1021 tgtgatggga cgcaacacct ttgaaaaagt gatcagtttt ggtggttggc attacgataa
     1081 aaaggtcttc gtgctttcca gccgtaggtt ggatatccct tcggagctaa acgaaaaagt
     1141 tgaagcgctt caccaaactc cccgagagtt gatccgcgaa ctggagcggc gcggcatccg
     1201 acatatttat ctcgatggcg gtgtgaccat ccaacgattt ttgcgcgaag ggttggtgaa
     1261 cgaaatgatt atcaccacga tccctgtttt gatcggtgaa ggtttacctc tgtttggtga
     1321 attagaaaaa gacattcagc tggaattact ggatgtaagt tctttctcga atgggcttgc
     1381 gcaaaagaaa tacaaagttc ttgcataaaa atcaaaagag cgtgattggt agcacgctct
     1441 tttgattcaa gattagaact tcaattggtc tattgccgag cggac
//
LOCUS       DAOREF010000849         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_81474_length_1484_cov_2.611617, whole genome shotgun sequence.
ACCESSION   DAOREF010000849 DAOREF010000000
VERSION     DAOREF010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81474_length_1484_cov_2.611617"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKI33_14940"
     CDS             complement(<1..621)
                     /locus_tag="PKI33_14940"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNM38363.1"
                     /translation="MKKISIIGAGNTGATAAHWIAERELADVVLLDVVEGMPQGKSLD
                     LLEAMPIIGKDSHVLGTNNYEDTKNSDIIIITAGIARKPGMSREDLLKTNAEIVGKAA
                     TETLKHSPNAIFIVLTNPLDTMAYLVMKKTGLPRERVIGQAGILDSARMRAFVAMEAS
                     VSVENVQCYVLGGHGDEMVPLTRHSNIAGVPLKEYFPADKLEAIVNR"
     gene            complement(855..>1484)
                     /locus_tag="PKI33_14945"
     CDS             complement(855..>1484)
                     /locus_tag="PKI33_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM38364.1"
                     /translation="DSVILATGANPVHLNVPGEYEMTGRGVSYCATCDGWFFKDKKVV
                     IVGGGDSAFEEALFITRYASTVTLIHRREEFRAGAILQRRAKEHPKMNFILNTVVTEV
                     NQGDKLTTLKLKNVQTNEESTFDTDGLFIFIGHTPNTDMFKGKLDMNELGYLKINDKM
                     ETSIPGVFAAGEVADATFKQVVTSAGMGAAAAIQATRYLESLEEEAAHS"
BASE COUNT          355 a          381 c          371 g          377 t
ORIGIN      
        1 gcggttaacg atcgcttcga gcttatcagc cgggaaatat tccttgagcg gcacacctgc
       61 aatattggag tgacgagtaa gcggaaccat ttcgtcgccg tgcccaccca acacatagca
      121 ttgaacattt tcaacagaaa cactggcttc catcgcaacg aaagcacgca tacgggcaga
      181 gtccaaaata cccgcctgcc caatgacacg ttcgcgcggt aaaccagttt tcttcatgac
      241 caagtacgcc atcgtatcaa gcgggttggt cagcacaata aaaatagcat tcggagaatg
      301 cttaagggtt tcagtagcag ccttgcccac gatctcggca ttggtcttga gcaagtcttc
      361 gcggctcatg ccgggcttgc gagcaatacc agcagtgata ataatgatgt ctgaattctt
      421 tgtgtcttcg taattgttgg ttcccaacac atgcgagtct ttcccaataa tcggcatggc
      481 ttcgagcagg tccaggcttt ttccttgcgg cattccctct acaacatcta gcaaaacgac
      541 atcggcgagt tcacgttcgg cgatccaatg cgcagcggtg gcgccggtat tgcccgcgcc
      601 aataattgag attttcttca tcgtacttcc tccaatggga tcggctaaaa agccgtattt
      661 ttttataatt ttatccgaaa gtgacctaga ccaaaagtac aaaatgtcac atttttggac
      721 aaatccactc aagaaaacag actcgccgct aaaagcgagt ctgttggtgg ataatgctta
      781 gaggatggct cctgcgcctt gggcgggatc agctgacccg tagagtgagg cgtggctcgt
      841 catccgcttc tttcttaact gtgggctgct tcctcttcca gcgactcgag ataacgggtc
      901 gcctgaatgg ctgccgcagc ccccatgccc gccgaggtga ccacttgctt gaaggtggca
      961 tctgccacct cccctgctgc gaacacgccg ggaatgctgg tttccatttt gtcgttgatc
     1021 ttgagatagc cgagttcgtt catgtcgagc ttgcctttga acatatcggt gttgggtgta
     1081 tgaccgataa agatgaacaa gccatctgtg tcaaaggtgc tttcttcgtt ggtttgaaca
     1141 ttcttgagtt tgagggtggt gagcttatcg ccttgattaa cttcagtgac caccgtgttc
     1201 aagatgaagt tcatcttggg gtgttctttg gcacggcgct gcaaaattgc acccgcacgg
     1261 aactcttcac gacggtggat gagggtaaca gttgaagcat agcgcgtaat gaagagagcc
     1321 tcttcaaaag cgctatcgcc gccgccgaca atgactactt tcttgtcctt aaagaaccag
     1381 ccatcacaag tggcgcaata ggaaacgcca cgaccggtca tttcatattc gccgggcaca
     1441 ttgaggtgca cagggttggc gccagtcgca aggatgacgc tatc
//
LOCUS       DAOREF010000850         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_81846_length_1480_cov_3.583860, whole genome shotgun sequence.
ACCESSION   DAOREF010000850 DAOREF010000000
VERSION     DAOREF010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81846_length_1480_cov_3.583860"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1480
                     /locus_tag="PKI33_14950"
     CDS             28..>1480
                     /locus_tag="PKI33_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM38365.1"
                     /translation="MTAQSRIDAQTNLILRIHVIWRGVAALLAAISIGVIWQGMAETP
                     LWQKILFSLVAVLVMGFSGFGAYQISKRNHAGRMISLTIDYLGFVICFVFALNVGEFF
                     IGIDALGENFGRGIPYLGITALGYFLGMLEEYFPTKNKDSESSLKTVSKWVMLGGFVL
                     FLWQVNALNGLIYFLGKLAQPSGIVAVVGLVIFGISLWSMSQPNIAQALNVKTGHEQV
                     INGWLFLSPNLLGFLIFFAGPLLLSFYFSFTDSDAIRTPNWVGFENYAKILNIKFVTL
                     DTPDQLARDAVDIKVYDEVGRFKFGDTGILFAAEDKFFWLALRNTLTFVLFAVPLSVI
                     PALILSNILNSKLPGMKFYRAVYFMPSIAAVVGISLVWQWLYNATIGYINYFITLGIE
                     FWNNLFNVAVVDPKIQWVSDEKTALFAIIIIAAWQTMGFNTVLFLAGLQNIPGELYEA
                     ATVDGAGAWDKFWGITLPMLAPTTFYVVSTTTIQ"
BASE COUNT          325 a          356 c          359 g          440 t
ORIGIN      
        1 gacgcccaat ttcacggaga tgagaatatg acagctcaat cacggataga cgctcagacg
       61 aatttaattc tgcgcatcca tgttatatgg cgcggggtgg ctgctttgct ggcagccatt
      121 tccattggag taatctggca gggaatggca gaaacacccc tatggcaaaa gatactattt
      181 tcgctggttg ctgtgttagt tatgggcttc agcggatttg gggcatacca gatctcaaaa
      241 cggaatcatg ctggtcgcat gatctccttg accattgatt atctcgggtt tgtcatttgt
      301 tttgtattcg ctttgaatgt aggcgaattt tttattggaa tcgatgctct cggcgaaaat
      361 tttggtagag gcattcctta tcttggcatc actgccttgg gctacttcct cggtatgttg
      421 gaggaatatt tcccaactaa gaataaagac tcagaaagca gcctcaagac cgtcagcaag
      481 tgggtgatgt tgggcgggtt tgtgctcttc ctctggcagg tgaatgcgct caatgggctg
      541 atctatttcc tcggcaaact tgcccaaccc agtggaatag tcgccgttgt cggtttggtg
      601 attttcggca tctcgctttg gtctatgagc cagccgaaca ttgcacaagc cttgaacgtc
      661 aagaccgggc acgagcaggt tatcaacggc tggttgtttc tttcgccaaa tttgctgggc
      721 tttcttattt tctttgcggg tccgttactg ctttcgttct atttctcatt cacagactca
      781 gatgccatcc gtacgccgaa ttgggtcggg tttgaaaatt acgccaagat cctcaatatc
      841 aaatttgtca ccctcgacac acccgatcaa cttgcccgtg atgccgtaga tatcaaagtc
      901 tatgatgaag tagggcgctt taaatttggg gatacgggca ttctgtttgc tgctgaagat
      961 aaattcttct ggctcgcgct tcgcaacact ctgaccttcg tgctgttcgc tgttcctttg
     1021 agcgtgatcc ccgctttgat cttatcgaat atcctcaata gcaaattacc tggtatgaag
     1081 ttctatcgcg ctgtgtattt catgcctagc atcgcggcag tcgtaggtat ctcgctcgtt
     1141 tggcagtggc tatacaacgc gaccatcggg tatatcaact actttattac cttgggcatt
     1201 gagttttgga ataatctgtt caatgttgct gtcgttgacc cgaagatcca atgggtttcc
     1261 gatgaaaaga cagcactctt tgccatcatc atcatcgccg cttggcagac gatgggcttc
     1321 aacaccgtgc ttttcctcgc gggcttgcag aacatccccg gtgagttata cgaagctgcc
     1381 accgtggacg gagccggagc ctgggacaag ttctggggca ttacgcttcc catgcttgca
     1441 cccacaacct tctacgttgt atccaccacg actattcaag
//
LOCUS       DAOREF010000851         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_82482_length_1474_cov_3.546159, whole genome shotgun sequence.
ACCESSION   DAOREF010000851 DAOREF010000000
VERSION     DAOREF010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82482_length_1474_cov_3.546159"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKI33_14955"
     CDS             complement(<1..407)
                     /locus_tag="PKI33_14955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM38366.1"
                     /translation="MAKTKVTTPVKKTKPAVKKEAVTKRPAKRSKPSSGKASVFENRS
                     FQQFIEFAPVAFIAIDENSIIQFVNAHAEDLFGYSRKDLIHSNFEMLIPERFRDTHAK
                     QKKAFLKTPQKRAMGSGLDIYGLHQSGKEIPVE"
     gene            complement(525..>1474)
                     /gene="rny"
                     /locus_tag="PKI33_14960"
     CDS             complement(525..>1474)
                     /gene="rny"
                     /locus_tag="PKI33_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNM38367.1"
                     /translation="VAEVTRAVVTLPSEEMKGRIVGRNGRNIKAFEQAAGVDVIVDDT
                     PDSVTISCFDSVRREIARRALSKLILDGRIHPAHIEKVIDDETRAVEKIINEAGEQAA
                     YDANVSGLHPEILRMMGRLKFRTSYGQNQLAHAVEVSKLAGILAAEIGANVELAKLGG
                     FLHDIGKAMDHNQEGTHAGLGAEFCKRYGVNAVVVNAIAAHHHEVDQETVEAVIAEAA
                     DAISGARPGARREDLEAYIKRIRSLEEMSLSFEGVQQAFAIQAGREVRILVKPETIDD
                     LGSTRLARDIAKKIEETMQYPGQIRVTVIRETRATEYAK"
BASE COUNT          340 a          377 c          362 g          395 t
ORIGIN      
        1 atttcaactg gaatttcctt gccgctttga tgcagaccat agatatccag ccctgacccc
       61 attgccctct tttgaggagt tttgagaaaa gctttcttct gtttcgcgtg cgtatcccga
      121 aacctttcgg ggattagcat ttcaaaattg ctgtgtatta aatctttgcg tgaataccca
      181 aatagatctt ctgcatgtgc gttaacgaac tgaataatac tgttttcatc gattgcaata
      241 aatgctacag gtgcaaactc aataaactgt tggaaagatc tgttctcaaa aacggaggct
      301 ttcccactag acggctttga tcttttggca gggcgtttcg tcaccgcctc ttttttgacg
      361 gcaggcttgg tcttttttac aggggtcgtt accttggttt tcgccatgac cttctcttta
      421 ataaacaggg ctgggactat aaactaaaaa cccatcccgc aaagtgcttg tattttagca
      481 ccctgtagga tgggttaagg tattcgtaac tagcttgtaa ggcgctattt ggcgtattcg
      541 gtggcgcggg tctcgcggat gaccgtcaca cggatctgac caggatactg catagtctct
      601 tcaatcttct tggcaatgtc acgggcgagg cgagtggaac cgaggtcgtc gatcgtttcc
      661 ggcttgacga ggatgcggac ttcacgtcca gcttgaatag caaaggcttg ttgcacaccc
      721 tcaaaggaca aggacatttc ttccagcgag cggatgcgct taatataggc ttcgaggtct
      781 tcacggcggg caccagggcg agcgccagag atggcgtcgg ctgcttcagc gatcacagct
      841 tccacagttt cctgatccac ttcatggtgg tgtgcggcga tggcattcac caccacagca
      901 ttgacaccat agcgcttaca gaattctgcg ccgagacccg catgtgtccc ttcctgattg
      961 tggtccatag ctttgccaat gtcgtgcaag aaaccgccta atttggcaag ctctacattc
     1021 gcgccaattt cagccgcgag gatgcccgct aatttagaga cttcaacggc atgggcgagt
     1081 tgattctgac catacgaggt acggaacttg aggcgaccca tcatgcgcaa aatttcggga
     1141 tgcaaaccag agacgttcgc atcatatgcg gcttgttcgc ccgcttcgtt aatgatcttc
     1201 tctacagcgc gggtttcatc atcgatcacc ttttcgatgt gagccgggtg aatacgcccg
     1261 tcgagaatca gcttggaaag tgcacggcga gcaatttcac ggcgcaccga gtcaaaacac
     1321 gagatggtca cagagtcggg cgtgtcgtcc acaattacat ctacaccagc tgcttgttcg
     1381 aaggctttga tgttacgtcc attacgacca acgatacgtc ccttcatctc ttcgctgggc
     1441 agagtgacca cggcacgagt aacttccgcc acat
//
LOCUS       DAOREF010000852         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_82710_length_1472_cov_5.025406, whole genome shotgun sequence.
ACCESSION   DAOREF010000852 DAOREF010000000
VERSION     DAOREF010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82710_length_1472_cov_5.025406"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKI33_14965"
     CDS             complement(<1..450)
                     /locus_tag="PKI33_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38368.1"
                     /translation="MKDTLKQIIPQPLWQFARNTYDGFRRIPQLPEAYFHPWRRESIK
                     RLSALKNIHKGKRAFIIGNGPSLKQTDLSKLKNEITFGMNRFYLAFPDLGFTTSYICV
                     TNDLVIEQFVNDFLALQVPQFIAWRSHRHYPANLQPSSLPTFIYTTYT"
     gene            complement(447..827)
                     /locus_tag="PKI33_14970"
     CDS             complement(447..827)
                     /locus_tag="PKI33_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38369.1"
                     /translation="MTRARKKLSNRFQSSLFLAKLLFYFNAAWWLLLGIIFVYQMVED
                     KNSWSSVMVSIFFVVMIASLIVGARIVSEREKWAFVLCIVIAVANILFSIFGFLDFLF
                     VIAALFDLAILGSLYSLKGYYLTS"
     gene            complement(851..1201)
                     /locus_tag="PKI33_14975"
     CDS             complement(851..1201)
                     /locus_tag="PKI33_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38370.1"
                     /translation="MKFLQPARYFLLLISAFLSVLSILTLTRLGERPGMVGWYAIYSI
                     FMLIDSVVVLVCYFRLKQQGQRIFQVSMLILLLNMVLTIFDQIGVVDVIFIVLNLFAL
                     ILLYMARKEIAPVK"
     gene            complement(1204..>1472)
                     /locus_tag="PKI33_14980"
     CDS             complement(1204..>1472)
                     /locus_tag="PKI33_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNM38371.1"
                     /translation="DNIRVNAILPGAVDTQMLRAGLGRGHVGAGDLQDRLDNLARKTV
                     NGRVGKPEEIAHAIYFLADNEQSSFMTGQSIIVDGGATARLSTE"
BASE COUNT          488 a          308 c          319 g          357 t
ORIGIN      
        1 ggtataggtg gtgtagatga aagtgggcaa actggatggt tgcaaattag caggataatg
       61 gcgatgtgag cgccaagcaa tgaactgcgg cacttgtaaa gcaagaaagt cattaacgaa
      121 ttgctcaatg acaaggtcat tcgtaacgca aatgtatgaa gttgtaaaac caagatcagg
      181 gaaagcaaga taaaaacggt tcatgccaaa ggtgatctcg ttcttgagct tgcttaagtc
      241 cgtttgcttt agagagggac cattcccgat aatgaaagcg cgtttgcctt tatgaatatt
      301 tttgagtgca gagagacgct taatgctctc acgccgccat ggatgaaaat acgcttcagg
      361 aagttgaggg atgcgacgaa agccatcata ggtgttgcgt gcaaattgcc agagaggctg
      421 aggaatgatc tgcttgagag tatccttcat gaggttaagt aatagccttt gagagaatat
      481 aaactaccaa gaatggcaag gtcaaataaa gcggcaatca caaaaaggaa atccaggaaa
      541 ccaaaaatgg aaaaaaggat atttgccaca gcaatgacga tacaaagcac aaatgcccat
      601 ttttcacgtt cgctcacaat gcgagcgccc actataagag aagcgatcat gaccacaaaa
      661 aagatggaca ccatcaccga tgaccaacta ttcttatctt caaccatttg gtatacaaag
      721 attatcccta ataaaagcca ccaagcggca ttgaaataga acaagagctt tgccaaaaag
      781 aggctggact gaaatcgatt gctcagcttt tttcttgcgc gtgtcatttt ctacagacct
      841 tttcttatat ttattttaca ggtgcaattt ctttacgtgc catatagagc aagatcaatg
      901 cgaataaatt caggacaata aaaataacat caacaacccc gatttgatca aatattgtca
      961 gaaccatatt caaaagcaga atgagcatac tcacttgaaa gatgcgttga ccttgctgct
     1021 tcaaccgaaa atagcagacc aacacaacta ctgaatctat gagcataaaa attgaatata
     1081 ttgcatacca acccaccatt cctggtcttt cgccaagacg agttaaggtc agaatactca
     1141 gtacagacaa gaaggcagat atcagtaaga gaaaataacg cgcaggttgt agaaatttca
     1201 tttttactcc gtgctgagac gcgccgttgc gccaccatcc acaataatgg attgacctgt
     1261 cataaatgaa gattgttcat tatcagcaag gaagtaaatg gcatgagcaa tctcttcggg
     1321 ctttcccaca cgcccattga ctgttttgcg ggcgagatta tccaaccggt cttgcaggtc
     1381 gccggcacct acgtgtccac gcccgagacc ggctctcagc atttgagtat ctacagcgcc
     1441 gggcaaaata gcattgacgc gaatattatc gg
//
LOCUS       DAOREF010000853         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_82758_length_1472_cov_2.563162, whole genome shotgun sequence.
ACCESSION   DAOREF010000853 DAOREF010000000
VERSION     DAOREF010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82758_length_1472_cov_2.563162"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..304
                     /locus_tag="PKI33_14985"
     CDS             77..304
                     /locus_tag="PKI33_14985"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNM38372.1"
                     /translation="MTETSQPNNPLHGITLETIVTQLVTQYGWAELGKRIPIKCFTND
                     PSIKSSLKFLRQTPWARKKVEDLYLQSIGKG"
     gene            313..807
                     /locus_tag="PKI33_14990"
     CDS             313..807
                     /locus_tag="PKI33_14990"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNM38373.1"
                     /translation="MRIGRMFFIVVLVICLFELARLWTISPLQMAAHFNALGEPDRFV
                     SKPEFFWGQVQTLLVVVLVSLPLQVLFLVIPPNLINMPNRDYWLAPERKKETLGRLSD
                     FGSMMFAVILLALLAGFEIATYANLQTPIHFNAGLMGVVMFAAMGVIVLLLIQLVLSF
                     KLPK"
     gene            complement(858..>1472)
                     /locus_tag="PKI33_14995"
     CDS             complement(858..>1472)
                     /locus_tag="PKI33_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38374.1"
                     /translation="TTVTVSIATNCRTGPGQAYPSIYGLPVGVVGKVLGNNPSVSNYW
                     VIEIPNSGGGTCWLWGQYATVTGDTSGLKVFAVPATPTPTASPSPTPTVTQAVAPTAP
                     TNANAVLLTCVTNNFVQSLTATFTWTDNSNNETGFNIYVNANFADGSSKDQLVGSVGP
                     NTTSYTVSTTALSKRAPIIKVEAFNNAGSSTRVSAQPLSLATCP"
BASE COUNT          337 a          343 c          395 g          397 t
ORIGIN      
        1 tcttccgtgt ggcagaaccg catcaggtca gcgggttatg gtttaacaag tagcacgcga
       61 atacactgta cctgctatga cagaaacttc acaacccaat aaccctttac atggcattac
      121 gcttgaaacg atcgtgactc aactggtaac acaatacggc tgggcagagt tgggcaagcg
      181 cattccgatt aaatgcttta caaatgatcc gagcattaaa tcgagcctca agttcttgcg
      241 gcaaacgccg tgggcgagaa agaaagtgga agacttatac ttgcaatcga ttggcaaagg
      301 ttaaggtgat ctatgcgtat cggacgaatg tttttcattg ttgtgttagt gatctgcctt
      361 ttcgagctag cacgtttgtg gacgatcagt ccgctgcaga tggcggcaca tttcaacgct
      421 ttgggcgagc cagaccgctt cgtctccaag cccgaattct tttgggggca ggtgcaaacc
      481 ttgttggtcg tcgtgttggt cagtttgccg ttgcaagttt tgtttctggt cataccaccc
      541 aacttgatca acatgcccaa ccgcgactat tggctggctc ccgaacggaa aaaagaaact
      601 ctcgggcgtt tgagcgattt cgggtcaatg atgttcgccg tgattctgct tgcgcttctg
      661 gctggttttg agatcgccac gtacgccaat ttgcaaacgc cgatccactt caatgcaggg
      721 ttgatggggg tggtgatgtt cgctgccatg ggggtgattg ttttgctgtt gattcaactg
      781 gtcttgtctt tcaaattgcc gaaataaaaa agagacggtc acaaaatgac cgtctctttt
      841 ttgaattaac gattcgccta agggcaggtt gcaagactca gaggttgggc agatactctc
      901 gttgaactgc cagcattgtt aaacgcctct accttgatga taggcgcccg cttactaaga
      961 gcagtagtgc taactgtata actggttgta ttcggtccta ccgagcctac tagttgatct
     1021 ttagaactgc cgtctgcgaa gttcgcattc acatagatat tgaacccggt ctcgttattc
     1081 gaattatctg tccatgtaaa tgtggctgta agactctgta caaagttatt ggttacacag
     1141 gtcaggagaa cagcattggc attggtcggt gcggtaggcg ctacggcttg tgtcacggtt
     1201 ggcgtgggag atggagacgc ggtgggcgtg ggtgtggcag gcacagcgaa aactttcagt
     1261 ccgctcgtgt cgcctgtcac ggtggcatat tgtccccaca gccagcaagt tccgccgccg
     1321 ctgttgggga tttcgatcac ccaataattg gatacagacg gattgttacc gagcaccttt
     1381 ccgaccacac ctaccggtag cccataaata ctcgggtagg cttgcccggg tcccgtgcgg
     1441 caattggtgg cgatgcttac ggtgaccgta gt
//
LOCUS       DAOREF010000854         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_82830_length_1471_cov_4.187853, whole genome shotgun sequence.
ACCESSION   DAOREF010000854 DAOREF010000000
VERSION     DAOREF010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82830_length_1471_cov_4.187853"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKI33_15000"
     CDS             <1..252
                     /locus_tag="PKI33_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38375.1"
                     /translation="IRPILTILSTGPATGDRALWTGPIIFNASGVSAPASTPTATATG
                     TTAAASATPTATSSTPTATTSAASATPTATGTPTPTSTP"
     gene            336..>1471
                     /locus_tag="PKI33_15005"
     CDS             336..>1471
                     /locus_tag="PKI33_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38376.1"
                     /translation="MKRFLILTMIVLTACTSTPATPTPASFFDASPVPPTLAVVSSPQ
                     PIQPTNQVLPAVTEPVALGSISETGELYFFLQPRLGGGKIELVKVSAACITDPINCPP
                     LEKIQVPFAFNFTINALSWSPDGKYAAFSYSSSATGSPTQLWLFDATAKTWRSLVEYP
                     YIDPPFWSPDGQWIAFRTQDGMGTEAVYIIHPDGSELKNIAADLPVEGRPYIMDGWYT
                     ENVIMRSALPGAQGNIYLVRAANGQSRPMFETLLTKAQFIASPDAGFFAYDEYDYASQ
                     NHFVKVMEPDGANAVILTQFIGGSIYPMIWSPDSNLLAFNYYGNMTSGNSFAEVFTIP
                     RRGGELKSVYKGNTVGRLLFSPNGKYLLIEETTSASGGHLYLVDL"
BASE COUNT          361 a          396 c          302 g          412 t
ORIGIN      
        1 attagaccaa tcctcaccat tctctctaca ggtcctgcaa ctggtgaccg cgctttgtgg
       61 acgggaccca ttattttcaa cgcatcgggc gtttctgcac cagcatccac tccaaccgcc
      121 acagctactg gcacaacggc agcagcctct gctacaccca ctgcaaccag ctcaacgcct
      181 actgccacaa cctcggctgc ttctgcaact cctacagcaa ccggaacacc aactccaact
      241 agcactccgt aatctgtaat gaaaaaagtt cccgttgtca gaagacagcg ggaacttttt
      301 aattccaaac tcgtcctttc acaaatggag ttttgatgaa acgatttttg attttgacca
      361 tgatagtgtt gacagcttgc acgtcaactc ctgctacgcc cactcccgct tcatttttcg
      421 acgcttcgcc tgttccgcct actctagctg ttgtttcttc tcctcagccg attcaaccta
      481 caaatcaagt cttacctgcc gtcaccgagc cggttgccct tggttcgata tcagagacag
      541 gcgaattgta tttcttccta cagcctcgcc ttggcggtgg aaaaattgaa ctggtaaaag
      601 tatctgcggc atgtattaca gaccctatca attgccctcc tttggaaaaa attcaagtcc
      661 cttttgcttt caacttcacc atcaatgctt tatcttggtc tccagatgga aagtatgcag
      721 ccttttcata ttcatctagt gctaccggaa gcccaaccca actctggctt ttcgatgcca
      781 ctgcaaagac atggcggtct cttgttgagt atccttacat cgacccgcct ttctggtcgc
      841 cagacggtca gtggatcgca ttccgcactc aagatgggat gggtacggaa gcagtttata
      901 ttatccaccc agatggcagc gagttaaaaa atatcgctgc agatttacct gttgagggga
      961 gaccctatat catggatggc tggtataccg agaatgtcat catgcgttct gctttgccgg
     1021 gtgcccaggg caatatctac ctcgtccgcg ctgcaaacgg acagtctcgc cctatgtttg
     1081 aaaccttact tactaaagca caattcattg catcacctga tgcgggtttc tttgcttacg
     1141 atgaatatga ttatgcatcc caaaatcatt ttgtgaaagt gatggaacca gatggagcaa
     1201 atgcggttat cctcactcaa tttattggcg gttcgatcta ccctatgatc tggtcaccag
     1261 atagcaacct gcttgccttt aattattacg gcaatatgac cagtggcaac tcttttgctg
     1321 aagtctttac catcccaaga agaggcggag agttgaagtc tgtttataaa ggcaacacgg
     1381 ttgggcgtct gctattttct ccgaacggaa aatatctgct tattgaagaa accacatcgg
     1441 cttctggtgg acacttgtat ttggtagacc t
//
LOCUS       DAOREF010000855         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_82868_length_1471_cov_2.290960, whole genome shotgun sequence.
ACCESSION   DAOREF010000855 DAOREF010000000
VERSION     DAOREF010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82868_length_1471_cov_2.290960"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..768
                     /locus_tag="PKI33_15010"
     CDS             142..768
                     /locus_tag="PKI33_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM38377.1"
                     /translation="MTIRIMIVDDHAIVRSGLKAILASQQDFELVADVGTGDAAVSAS
                     ATLRPDVILMDLQMPVMDGVTAISLIHQKQPDIRMLVLTTYDTDADIVRAVEAGAIGY
                     LLKDALPDELFRAIRSAARGEVIFAPSVAAKLAKRLTNTLDRSLSNREIEVLILAARG
                     DSNKEIASKLYITEATVKSHFVHIFNKLSVTDRTAAVTLALEKKLIRL"
     gene            complement(769..>1471)
                     /locus_tag="PKI33_15015"
     CDS             complement(769..>1471)
                     /locus_tag="PKI33_15015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNM38378.1"
                     /translation="APMSSQKPALDPMISSPDSNEYFNSISHLIGAILSISALAVLVS
                     LAAIDGKPAHVVGFAIYGTSLFLSFLFSCLLHFFLLFNKYYRVFGILDHNAIYILIAG
                     TYTPFCLTIFSGATGWVLFGVIWSLAVFFITLKSIFFAKIPVWLSQASFLLMGWIVVF
                     LIKPAYTQLGMDAIILMIAGGLSYTVGAISFAIGKPNPFPPYFGNHEIWHICVLAGNT
                     FMFLVMLWHILPYPK"
BASE COUNT          429 a          307 c          379 g          356 t
ORIGIN      
        1 gtttgaacaa agaaatctta agggctttgg tcttaaaacc atgcgtgaac ggatagaaga
       61 aataggtggc tctcttgtga tcgaaagtca attcggtgcg ggaacaacaa ttgccgtctc
      121 tttgcctctt gaggagcaag gatgaccata cgcattatga ttgtggatga ccatgcgatt
      181 gtgcggagtg gattgaaagc gatcctcgcc tcacaacaag actttgagtt ggtcgctgac
      241 gttgggaccg gagacgccgc tgttagtgct tctgccactc ttcgccccga tgtgattttg
      301 atggatttgc aaatgccagt tatggatggg gtgacggcaa tttcgcttat tcaccaaaaa
      361 cagccagata ttcgtatgct tgttcttaca acatatgata ccgatgccga tattgtacga
      421 gctgttgaag ctggggcgat agggtatcta ttgaaagatg cattacctga cgaattgttt
      481 cgggcaattc gttctgccgc gcgtggcgag gtgatttttg ctcccagtgt tgccgctaaa
      541 ttggcgaaac gcttaacgaa tacgcttgat aggtctctta gtaatcgcga gattgaagtg
      601 cttatactgg ctgcccgcgg tgactccaat aaagaaattg ccagcaaact gtatattaca
      661 gaagctacgg ttaaatcgca ttttgtgcat atctttaaca aactcagcgt cacagaccgt
      721 actgcggctg taacgctggc gcttgaaaag aaattgattc ggttgtagtt atttggggta
      781 aggcagtata tgccatagca tcacaagaaa cataaacgtg ttcccagcta agacgcaaat
      841 atgccagatc tcatgattcc caaaatacgg tgggaaagga ttgggcttac caatggcaaa
      901 gctgatggca cccacggtgt agctcaaccc gcccgcaatc ataaggataa tcgcatccat
      961 gccgagttga gtataggcag gctttattag aaagaccacg atccaaccca tgagcaagaa
     1021 tgaagcctgc gaaagccaga ccgggatctt ggcgaagaaa atagatttga gcgtgatgaa
     1081 gaaaaccgcc aagctccaaa tgacgccaaa gagcacccaa cctgtcgcac cgctgaaaat
     1141 ggtgagacag aaaggcgtat acgtgcctgc gatcaaaata taaatagcat tatggtcgag
     1201 gatgccaaag acgcggtaat acttgttgaa cagtaaaaag aaatgcagta aacaactaaa
     1261 taggaacgat agaaataaac tcgtgccata gatggcaaat cccaccacat gggcgggctt
     1321 gccatctatg gctgccagtg aaactaatac tgccagtgcc gagatcgaaa ggattgcacc
     1381 gatgagatga cttatggagt tgaaatactc gttgctatct ggtgacgaga tcatggggtc
     1441 gagagcgggt ttttgtgaag acatgggcgc g
//
LOCUS       DAOREF010000856         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_83265_length_1467_cov_3.377479, whole genome shotgun sequence.
ACCESSION   DAOREF010000856 DAOREF010000000
VERSION     DAOREF010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83265_length_1467_cov_3.377479"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..>1467
                     /locus_tag="PKI33_15020"
     CDS             119..>1467
                     /locus_tag="PKI33_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38379.1"
                     /translation="MIRKITFIHLAVICALLLTACGTSAASTQETVSATDTSSVVFIT
                     QTAEPTTQTADALPAGGVPNGNPPPGNPSGGGNMGGNLEDGLATATGIYTLSGSVAEE
                     TNQTYTATQQDQSGVYVTDGGVLTLNNVTVNTSGETSSSDDSSFYGLNAGILAVESTI
                     TVNGGTITTSGSGANGAFATGEGSVVNLSNVTITATGDGGHGVMATLGGEINLTNVNM
                     TTSGAHSAPIATDRGSGTVNSTGGSLLTTGADSPCYYSTGVLNIENSTCTADGSEAAV
                     IEGANSINLIDSDVTVNVPDKWGVMIYQSFSGDAEGTEGVFTMQGGSLTYTDANSPLF
                     YVNNSTGYITLDDVAVNVASGILVNAAANDRWGTSGQNGGTVYLTAINQTLNGDFVAD
                     TISSISVELTSNSSWIGAINANNEASSSSLALDASSTWTVTADSYLTCLSDPEGISGT
                     "
BASE COUNT          395 a          324 c          347 g          401 t
ORIGIN      
        1 aatgaaattc ttaagctgag aatgtactca cagcatattc acagtttctt ctttgcattt
       61 ccataggttc acattctaga atgcatatgc ttgaaaatat attctttgga gaaccaatat
      121 gatacgcaaa ataacgttta ttcatctggc tgtcatttgt gccttgttat taaccgcttg
      181 cggcacaagt gcggcatcta ctcaggaaac tgtttcggct acagatacat cctcagttgt
      241 ttttatcact caaactgctg agcctacaac ccaaaccgca gatgcgcttc ctgctggggg
      301 agtaccgaat ggcaatccgc ctcccggaaa cccgtccggt ggcggtaaca tgggtggaaa
      361 cttagaagac ggcttggcta cggctacagg tatttacaca ttgagcggca gtgtggctga
      421 agaaaccaat caaacatata cagccacaca gcaggatcaa tctggagtgt atgtcaccga
      481 tggtggtgta ttgaccttga acaatgtcac ggttaatacc agcggagaaa cttcttcatc
      541 tgatgatagt agtttttatg gcttgaatgc aggcattttg gcagttgagt caacgattac
      601 ggtcaacggt gggacgatca ctaccagcgg ctctggcgca aacggtgctt ttgcaacggg
      661 cgaaggctca gtggtaaatc tgagcaatgt taccattacc gcaaccggag atggtgggca
      721 tggggtaatg gcaacccttg gcggagaaat aaacctaacg aatgtgaata tgaccacttc
      781 tggtgctcat tctgctccta ttgcaactga tcgtggcagt ggtacagtca actctactgg
      841 cggttcttta cttacaactg gagcggattc accttgttac tattctacag gtgtcttgaa
      901 tattgaaaat agcacatgca cagcagatgg ttccgaagct gctgtaattg aaggtgctaa
      961 ttccattaat ctaattgata gtgatgtgac ggttaatgtc ccagataaat ggggcgtaat
     1021 gatctatcag agcttttctg gtgatgcaga aggaactgaa ggtgtcttta ccatgcaagg
     1081 cgggtcgttg acatacacag atgccaatag tcccttgttc tatgtaaaca attcaaccgg
     1141 atacattact ctcgatgacg ttgcggtaaa tgttgcatct ggaatcctcg tgaatgcagc
     1201 tgcaaatgat cgctggggta ccagtggaca gaacggtgga acagtctact tgaccgccat
     1261 caaccagacc ttgaacggtg actttgttgc agatactatt agctcaatct ctgttgaatt
     1321 gactagcaac tcttcatgga tcggagccat caatgcaaat aatgaagcaa gttcatcttc
     1381 cctggcatta gacgcctcaa gtacctggac cgtgacagct gactcttacc tcacttgctt
     1441 gagtgacccc gaaggcattt ctggtac
//
LOCUS       DAOREF010000857         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_83713_length_1463_cov_3.261364, whole genome shotgun sequence.
ACCESSION   DAOREF010000857 DAOREF010000000
VERSION     DAOREF010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83713_length_1463_cov_3.261364"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKI33_15025"
     CDS             complement(<1..821)
                     /locus_tag="PKI33_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNM38380.1"
                     /translation="MFVTKNLYQNDEAWKNVPLGFSSQTIGSWGCLLTSVTMMLNGIG
                     YNETPDTVNTKMKNAGGFQGPLFIPSVLPYVWGNCAYRDMQPCESVPAPLQQIDDAVA
                     SGKPVILQVDWNKQAGIQTHFVLVKEKKGDDYVLYDPYKYGGDGPDKEVLLTTRYKYN
                     GAKLSTEISAVLWFENYGINPPEPPAKVPVPAENFILYAAEDDLALRSQPATTGYLWK
                     RMVMNTELICLEPKAQAAAKIGVQGQWIQVQDPKGDQGYVASWFVSDKKAVPAPAT"
     gene            complement(1025..>1463)
                     /locus_tag="PKI33_15030"
     CDS             complement(1025..>1463)
                     /locus_tag="PKI33_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38381.1"
                     /translation="LPDQANGVSPELSKEVGEFLTKAIKLQIEAYRQGDASVASVIMA
                     GDVLQSLQGEISSLNQQGLVEIADIDYYKSYIDDIRIISNAQLEVDTCEVWSLQYYRR
                     SDGALVQSNEPTLLPQTITIQKIENGWFITAVQFFEAPAFCHS"
BASE COUNT          362 a          361 c          348 g          392 t
ORIGIN      
        1 gttgcaggag caggcacggc ttttttgtca gaaacgaacc aactcgccac atatccctga
       61 tcgcctttcg ggtcttgtac ctgaatccat tgaccttgca cgccgatctt ggcggcggct
      121 tgggctttgg gttcaaggca gatgagctca gtattcatca ccatgcgctt ccacaggtag
      181 ccagtggtgg cgggttgaga acgcagagcc aaatcatctt cggcggcgta tagaatgaaa
      241 ttctcagcag gaacgggaac cttggcaggt ggttcaggcg ggttgatgcc gtagttttcg
      301 aaccataata cagcactaat ctcggtggag agtttggcac cgttgtattt gtagcgggtg
      361 gttagcaata cttctttatc tggaccgtcg ccgccgtatt tatacgggtc ataaagcaca
      421 taatcatcgc ccttcttttc tttcacaagg acaaagtgag tttgaatacc agcttgtttg
      481 ttccagtcca cttgcaaaat gacgggtttg ccagaagcaa ccgcatcgtc gatctgttgc
      541 agcggagccg gaacagattc acaaggctgc atatcgcggt atgcgcagtt tccccaaacg
      601 taaggcaaca cactgggaat gaagagcgga ccctgaaaac cacccgcgtt cttcatctta
      661 gtgttgaccg tatcgggcgt ttcgttgtac ccgatgccgt tcaacatcat agtcactgaa
      721 gtgagcaaac aaccccaaga accgatggtc tgcgatgaaa aaccgagcgg aacgttcttc
      781 caggcttcat cattttggta caagttcttc gtaacaaaca taaaaaattg cctcctccat
      841 ttggagattg ggaatttcaa tcttgctttt taaggataaa tttatctaat tctactcgat
      901 ttgcctttgc aacgattttg caatatttta aggtggatgc tgtccggctc tctctccgcc
      961 gcctcttggc tcaccccatt ggcagttttt tcccacataa atcgacctgc caatggggcg
     1021 cattttacga atggcagaaa gccggtgcct caaaaaattg aaccgcagtg ataaaccagc
     1081 cattctctat cttttgaata gtgatcgttt gtggcaatag cgtaggctca ttgctttgaa
     1141 caagggcacc atctgaacgg cgataatact gcaaagacca gacttcacaa gtatccactt
     1201 caagttgagc attgctgatg attcgaatat cgtcgatata gcttttgtaa tagtcaatgt
     1261 ctgcgatctc gaccaacccc tgttggttga gcgaggaaat ctctccctgt agagactgta
     1321 gcacgtcgcc agccatgatc acagacgcaa cagaagcatc gccttggcga taggcttcaa
     1381 tttgcagctt gatcgccttc gtgagaaatt cgccgacttc cttcgaaagc tccggcgaga
     1441 ctccattggc ttggtctggc agg
//
LOCUS       DAOREF010000858         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_84016_length_1460_cov_5.018505, whole genome shotgun sequence.
ACCESSION   DAOREF010000858 DAOREF010000000
VERSION     DAOREF010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84016_length_1460_cov_5.018505"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PKI33_15035"
     CDS             <1..876
                     /locus_tag="PKI33_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HNM38382.1"
                     /translation="AIGAKGGFGLMVPILAGYIAFSIADRPGLAPGMIGGILAGVTGS
                     GFLGGIIAGFLAGYFTQWLNGAIKLPRNLAGLKPVLILPLISSLVVGLIMFYVVGVPV
                     SAALKALTGFLTGMQTGSAAILGLILGLMMAFDMGGPVNKAAYTFSVGLLSTQVTAPM
                     AAVMAAGMTPPLGIALATYLFKNRFTEDERESASATAVLGISFITEGAIPYAARDPFR
                     VIPSIMVGSGVAGALSMAFGCQLHVPHGGIFVLPIPNAVDNLGMYIVSLLVGTVVTAG
                     MLYVLKKPVEQEATA"
     gene            950..>1460
                     /locus_tag="PKI33_15040"
     CDS             950..>1460
                     /locus_tag="PKI33_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNM38383.1"
                     /translation="MKTLITGATGFLGGALAQRLHGMGWNVTALGRNPKKLNGLEDNG
                     MHVVRADISKKDEVTASFSDFDLVFHCAAFPSPWGSFEKFYQANVIGTRNIVQACLEN
                     KVKRLVYVSTPSIYFDYNSRVNVKENDVLPEPISHYAHTKSLAEEEVDKGFQNGLAVI
                     SIRPRALFGE"
BASE COUNT          291 a          428 c          357 g          384 t
ORIGIN      
        1 gccatcggcg ccaagggcgg ctttggtctt atggttccga tcctcgctgg gtacatcgca
       61 ttctccatcg ctgaccgccc cggtctcgct cccggtatga tcggcggcat ccttgctggt
      121 gtcaccggtt ccggcttcct cggcggtatc atcgctggct tcctcgctgg ttacttcacc
      181 cagtggctca acggtgcgat caaacttccg cgcaaccttg cgggtctcaa acctgtgctc
      241 attctgcccc tcatcagctc gctcgtcgtt ggcttgatca tgttctacgt ggtcggtgtg
      301 cctgtcagcg ccgcgctcaa agccctcacc ggcttcctga ccggcatgca aactggcagt
      361 gctgccattc tcggcttgat cctcggcttg atgatggcat ttgatatggg cggtcctgtg
      421 aacaaggcag cctatacctt ctccgttggt ttgcttagca cacaagttac tgctccgatg
      481 gcagcggtta tggcggctgg catgacccct ccgcttggca ttgcccttgc tacctacctc
      541 ttcaagaacc gcttcaccga agatgaacgc gaatccgcca gcgcgactgc cgtgcttggc
      601 atctccttca tcaccgaagg cgccatccct tacgccgctc gtgacccgtt ccgtgtcatc
      661 ccttccatca tggtcggttc cggtgttgct ggtgcactct ccatggcgtt cggttgccaa
      721 ctccatgttc ctcatggtgg catcttcgta ctgcccatcc ctaacgctgt agacaacctc
      781 ggcatgtaca tcgtttccct gctcgtcgga actgttgtca ctgctggcat gctctatgtg
      841 ttgaagaaac ccgtcgaaca agaagctaca gcatagttca tacaaatgtt tttatccctc
      901 gtccgtgctg gcgagggatt tttttgttaa ttacggatac aatcgatata tgaaaaccct
      961 catcactggt gcaaccggat ttttaggcgg agcattggcg caacgcctgc atggcatggg
     1021 gtggaacgtc accgcccttg gacgtaaccc taaaaaatta aatggacttg aagacaacgg
     1081 catgcatgtt gttcgtgctg atatctccaa aaaggatgaa gtgaccgcct ctttctcaga
     1141 ctttgacctg gtctttcact gtgcggcatt tccctcgcct tggggtagct tcgaaaaatt
     1201 ctatcaagcg aatgtgattg gcacccgcaa catcgtgcag gcttgccttg aaaacaaggt
     1261 caagcgcttg gtctacgtct ctacgccaag catatatttt gactacaact cacgtgtaaa
     1321 cgtcaaagag aatgacgttt tgcccgagcc catcagccat tatgcgcaca caaaatcact
     1381 cgctgaagaa gaagtagaca aaggcttcca aaacggactg gctgtgatct ccattcgtcc
     1441 gcgggcgctt tttggagaag
//
LOCUS       DAOREF010000859         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_84017_length_1460_cov_4.944484, whole genome shotgun sequence.
ACCESSION   DAOREF010000859 DAOREF010000000
VERSION     DAOREF010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84017_length_1460_cov_4.944484"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..630)
                     /locus_tag="PKI33_15045"
     CDS             complement(172..630)
                     /locus_tag="PKI33_15045"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM38384.1"
                     /translation="MSTLFLICGLPGAGKTTLAKELEESEPALRLCPDEWIQPILSDP
                     NNRQEMDRLRGHIEAIQWELAKRSLSLGVHVILEFGFWSKQERANFRKEAETIGANVR
                     LIYLDVEPEELWKRLEKRNANLTKGSFHVEKDELLDWVQLFEAPTPDEFA"
     gene            683..1294
                     /locus_tag="PKI33_15050"
     CDS             683..1294
                     /locus_tag="PKI33_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38385.1"
                     /translation="MEYLLTSSPILGMLLWITLYISDYYMTLSSAKGYKEMSVMRFEN
                     SFELNPAFQKDIDSQTKVSSRHVTFLILYTAAILFLWFVAVRMFNAAWAYSFFIGMLV
                     LMEVTIHMRHFRNWYFLRVFRSKGGITGGITYAQWFSYQVSAFEFYCFAALYLFFFLL
                     TYSFFFLGGVMICLRIGVSHSNLAKKAERQHSASVAKNENSSS"
     gene            1275..>1460
                     /locus_tag="PKI33_15055"
     CDS             1275..>1460
                     /locus_tag="PKI33_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNM38386.1"
                     /translation="MKILHRDSSIVVVDKPADLSVLAEGWSKDAPYLAKILGEQFGEI
                     WVVHRLDKGTSGVMVFAL"
BASE COUNT          354 a          299 c          347 g          460 t
ORIGIN      
        1 aatattctct tcatcgtcaa tgagtatctg attgagctcc ctgcgccctt cgcgagtaag
       61 cagtttgcga atgggacccg ccagcgggtg attgagcgaa tcgacaagaa aagggttgtt
      121 ggctgattgt tcttccatat tggtcatgat aacgccctaa cggaatgagt gtcaagcaaa
      181 ttcgtctgga gtgggggctt caaagagttg tacccagtcc aacagttcat ccttttcaac
      241 atgaaagctg cctttggtaa ggtttgcgtt tcgtttttca agccttttcc atagctcctc
      301 aggctctaca tctaagtaaa tcaggcggac gttcgctccg atggtttcgg cttcttttcg
      361 aaagttcgcc cgttcttgct tcgaccagaa tccgaactca agaataacgt gaacgcccaa
      421 agagagagaa cgtttggcaa gctcccattg aatagcttcg atgtgaccgc gcaatctatc
      481 catttcttgg cggttatttg ggtcagataa gatgggttga atccattcgt ctgggcagag
      541 gcgtagggca ggctctgatt cttccaattc tttagcgaga gtcgtttttc cggcgccggg
      601 taacccacaa atcaggaata aagtagacat gcttatacat tgtaataggg gagcaggtaa
      661 aattagagtc agaggagttg atatggaata cctgctcacg agttccccca ttcttggcat
      721 gcttctatgg atcacgcttt acatcagcga ttattacatg accctttctt ctgcgaaggg
      781 atataaagaa atgagcgtga tgcgctttga gaatagcttt gaattaaacc cggcttttca
      841 aaaagatatt gatagccaaa ccaaagtttc aagtcgacac gtcacgttct tgatcttgta
      901 tacagctgcc attctttttc tatggtttgt tgctgtaaga atgttcaatg cagcgtgggc
      961 atattccttt ttcatcggta tgcttgttct catggaggtg accatccata tgcgtcactt
     1021 tcgtaattgg tattttttga gggtatttcg tagcaaaggc ggaatcacag gtggtatcac
     1081 gtatgctcag tggttttcat atcaggtatc tgcttttgaa ttctattgtt ttgccgcctt
     1141 gtatttgttc ttcttcttac ttacctacag ctttttcttt ttaggcgggg ttatgatttg
     1201 tttacgtatt ggagtgtctc attccaatct ggcaaagaaa gctgaacgcc aacactctgc
     1261 ctctgtagct aaaaatgaaa attcttcatc gtgattcgtc tattgtagtg gttgataaac
     1321 cagccgatct ctctgtcctt gcggaagggt ggagtaagga tgcaccgtat cttgcaaaaa
     1381 ttttgggaga acaatttggc gaaatttggg tggtgcatcg tttggataaa ggcacaagcg
     1441 gagtgatggt ctttgcactg
//
LOCUS       DAOREF010000860         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_84506_length_1456_cov_4.520343, whole genome shotgun sequence.
ACCESSION   DAOREF010000860 DAOREF010000000
VERSION     DAOREF010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84506_length_1456_cov_4.520343"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKI33_15060"
     CDS             <1..711
                     /locus_tag="PKI33_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38387.1"
                     /translation="KEANKMGKSNSSVETETQPAAATPPLAGIEVKADTPEPTADELG
                     RLRDILFGSQSRTLEKRLSDLESGLRSLRQEMTNLLQDQVGALSESTSTQFTETRRDL
                     NEKIDKQGMDQSAQLRASQKELTEKLEKQSAEQSAQLSAGQKQLSDNLEKLAADLLRQ
                     LRETHKELSDRLDKQNAEHMERTRTLQTEARQRDDGLRQELLSIAASLENKKTSRQDL
                     GQMLMELGLRLRQETDNS"
     gene            811..>1456
                     /locus_tag="PKI33_15065"
     CDS             811..>1456
                     /locus_tag="PKI33_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38388.1"
                     /translation="MNPLQPDTPSSERPEDDSLNPLISIRSILLSEVFERLRDIERQI
                     EASKNQSQSDDESLRQQLDELLAELDRLQRVAREADARSRDLHTEIEILRRRAQADSE
                     GLIARVTPVLGDMISRTIRDSRDEMAEALGPIMGEAIRVQIRDSRKDMVEALYPVIGE
                     SVQRAVGEFAKEFQRNIDARLKASFGPQSMVRTTMARLRGVSPAQLALRDSLPFS"
BASE COUNT          410 a          377 c          345 g          324 t
ORIGIN      
        1 aaggaagcaa acaaaatggg caaatcaaat tcttccgtag aaaccgaaac gcaacccgcc
       61 gcggcgacgc ctccacttgc tggtattgaa gtaaaagcgg atacacccga accaacagcc
      121 gatgaactgg ggcgtttgcg cgatattttg tttggctcac aatcgcgcac ccttgaaaaa
      181 agattgagcg atctggagtc tgggcttcgg tctctacgcc aggaaatgac caacctattg
      241 caagaccaag tcggggcgct ctccgaatcc actagcacac aatttacaga aactcgccgc
      301 gatcttaatg aaaaaatcga taagcaaggt atggaccaat ctgcccaact gcgcgcctcg
      361 caaaaagagc tcactgagaa gttggaaaaa caatcagcag aacaatctgc tcaattaagc
      421 gctggtcaaa aacaattaag cgataatctg gaaaaacttg ctgctgatct cctgcgccag
      481 ctccgcgaaa ctcataagga actcagcgat cgtttagata agcaaaacgc tgagcacatg
      541 gagcggacac gcaccctgca aacagaagcc cgtcagcgtg atgatggctt aaggcaagag
      601 cttctttcca tagcagcctc tttagaaaat aaaaaaacct ctcgccaaga cctaggtcaa
      661 atgctcatgg aattggggct acgtttacga caggaaactg ataattcctg atccccttcc
      721 attgcatgtg caataccttg tttattattt tcaacatatc aggtgacggc ttactaggac
      781 acctacagct ttttcgattt aagaaatttc atgaatcctc ttcaaccaga caccccatca
      841 tctgaaagac ccgaagacga ttctcttaat ccgttaattt ccatacgttc aatcctgctc
      901 tcagaagtat ttgagcgtct acgtgatatt gaacggcaga tcgaagcctc caaaaatcaa
      961 tctcaatctg atgatgaaag cctccgccaa cagttagacg aactgctggc ggagcttgac
     1021 cgcttgcaac gtgtcgctcg tgaagcggat gcgcgttcgc gagacctaca taccgaaatt
     1081 gagatactgc gccgcagagc tcaagccgac tcggaagggc taatcgcgcg cgtcacaccg
     1141 gtattggggg atatgatcag ccgcacgatt cgcgactcac gagacgagat ggctgaggcg
     1201 ctgggtccga tcatgggaga agcgatccga gtgcagatcc gtgattcgcg caaggacatg
     1261 gtcgaggctt tgtatcccgt tattggtgaa agcgtacaac gggcagtcgg cgagtttgca
     1321 aaggaattcc aacgcaatat tgatgcgcgc ttgaaggcat cctttggtcc ccagagcatg
     1381 gtccgtacaa ccatggcacg cttgcggggc gtttctcctg ctcaactggc tttgcgtgac
     1441 tctctgccgt tttcca
//
LOCUS       DAOREF010000861         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_85042_length_1451_cov_5.300143, whole genome shotgun sequence.
ACCESSION   DAOREF010000861 DAOREF010000000
VERSION     DAOREF010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85042_length_1451_cov_5.300143"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1019
                     /locus_tag="PKI33_15070"
     CDS             99..1019
                     /locus_tag="PKI33_15070"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNM38389.1"
                     /translation="MDLTKHAFFEGKIVPLGEAKINIATHGFLYGTAVFSGMRAYWNE
                     EKKGLYLFRPYDHFRRLLDSARMMAMEVPYDEEGLIQLTIDLLKTDNYQQDVYLRPTI
                     YKADMGIGVRLHNLRDELSIFTAAYEPYVKNDTNSHVTVSSWRRVDDNAIPARSKVAG
                     AYVNSALAKTDANRAGFDEALVLTSEGHISEGSAMNVFMVRNGTLYTPPLTDNILEGV
                     TRRSVIEVAQKELGIEVVERSIDRTEILIADEVFLTGSAAQVVAVTKIDYRPIGSGVM
                     GPITSKLRDIYEDIVRARNSKYANWNVIVK"
     gene            complement(1056..>1451)
                     /locus_tag="PKI33_15075"
     CDS             complement(1056..>1451)
                     /locus_tag="PKI33_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38390.1"
                     /translation="PTATKTPKAPFSATVTYIDSTIIHPEAACNWQGVAGTIIDANNA
                     DMLGIAVRLTGFYNGKSKNELTVSGIAPAYGKSGFELFLSAVPIDSDNLLTIQILDQA
                     GLPLSDNIPIKTYADCTKNMVLVKFKKNP"
BASE COUNT          397 a          315 c          339 g          400 t
ORIGIN      
        1 gcccgaagcg gactctctga gtcggtgtta tacatggtgg tatgcaatgc ccgcactggg
       61 tataatcctg accacaactc agcacaaagg aggtgcaagt ggatctcaca aagcacgctt
      121 tttttgaagg gaaaattgtt cccttgggcg aggcaaagat caacattgcc acgcatggtt
      181 ttttatatgg aacggctgtc tttagcggaa tgcgcgctta ttggaatgaa gaaaaaaaag
      241 ggctttatct ctttcgccct tatgaccatt ttcgccgttt gctcgattct gcgcgaatga
      301 tggcaatgga agttccttat gatgaagagg ggttgataca acttaccatc gatcttttga
      361 aaacagataa ctatcaacag gatgtttatt tacgtccaac aatctataaa gcagatatgg
      421 gcattggtgt gcgattgcac aacctacgcg atgaactctc gatctttact gcagcctatg
      481 aaccctatgt caaaaatgac accaactcgc atgtcacagt ttcttcctgg cggcgcgtgg
      541 acgataatgc tattccggcg cgcagtaaag tagccggggc ttatgtcaac tctgcgctgg
      601 caaagactga cgcaaatcgt gccgggtttg atgaagcttt ggtattaaca tcagaaggtc
      661 atatctctga ggggtctgcc atgaatgtgt tcatggtccg caacggcacg ctgtacactc
      721 ccccacttac cgataacatc cttgaaggcg ttacccgtcg ttccgtgatt gaagtggctc
      781 aaaaagaatt aggcattgaa gtcgtagagc gttccattga tcgcaccgag atcttgattg
      841 cggatgaagt cttcttaact ggcagtgcag ctcaggtcgt cgctgtaaca aagatcgact
      901 atcgccccat tgggagcggt gtaatgggac cgatcacttc aaagttacga gatatctatg
      961 aagatatagt gcgtgccaga aattcaaaat atgcaaattg gaatgtaatt gtgaagtaaa
     1021 taaaaaatcc gccgaatatt cggcggattt tttatttatg gattcttctt gaacttcacc
     1081 aacaccatgt tctttgtaca atctgcatag gttttaatcg gaatgttatc cgaaagcggc
     1141 aaacctgcct gatcaagaat ttgaatcgtt agtaaattat cagagtcaat gggcaccgca
     1201 cttaagaaca attcgaaacc tgatttcccg tatgcaggag caataccgct cactgtaagt
     1261 tcgttcttgc ttttaccgtt atagaatcct gtaagccgga cagcaatacc aagcatatct
     1321 gcattatttg catcaataat agtcccagct acgccttgcc agttacaagc agcttctggg
     1381 tggataatgg tgctgtcaat atatgtcaca gtagcagaga agggcgcttt cggcgtcttt
     1441 gtagcagttg g
//
LOCUS       DAOREF010000862         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_85496_length_1447_cov_4.613506, whole genome shotgun sequence.
ACCESSION   DAOREF010000862 DAOREF010000000
VERSION     DAOREF010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85496_length_1447_cov_4.613506"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..609)
                     /locus_tag="PKI33_15080"
     CDS             complement(16..609)
                     /locus_tag="PKI33_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNM38391.1"
                     /translation="MNISHLLEKSMKDHDHLCPRQILGVRIGLAGMKALGFSEPPQHK
                     QMLVISEIDGCFVDGVIAATDCTVGHRTLRVEDYGKVAVTFVHVKTGRAVRIAPALDI
                     REQAALFVPSEPRHYFAQMQAYQTMPDETMLNITEVELTTPIQEIVSRPGVRVNCDVC
                     GEEIMNEREVHSNGHVFCQSCYSHESYYHSVIRQKFP"
     gene            complement(734..>1447)
                     /locus_tag="PKI33_15085"
     CDS             complement(734..>1447)
                     /locus_tag="PKI33_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNM38392.1"
                     /translation="DMSKEPKIRLAGAGALLVAAIAVVAIGAPSLADRYNKLTFTRTE
                     TVEQLNADPIINTYVYTADQMLEKRLVFITPAEAYKAKYNQAMNPIYLDVRSEADYNL
                     YHIEDAVNVPLERITEIVPMLLSEPPTNSVFIVMSNDEAAAIQAWKLLSASSVSNVYI
                     LEGGVNNWIAFFGKDDTTFTAQPNPNAGDDELAYIFPSALGSRYESCSPNPVEYEALE
                     FEAKIKLQLKRDKSGGGCG"
BASE COUNT          376 a          340 c          343 g          388 t
ORIGIN      
        1 tatagctgga tattttcacg gaaatttctg ccgaataaca gaatggtaat agctttcatg
       61 agaatagcaa ctttggcaaa atacatgacc gttgctatgg acttcacgct cattcatgat
      121 ctcttcaccg catacatcac agttgacccg tactccggga cgcgagacga tttcttggat
      181 gggagttgtc aattccacct ctgtgatgtt caacatggtt tcatcaggca tagtctgata
      241 cgcctgcatt tgagcaaagt aatgacgtgg ttcgcttggc acgaataaag cggcttgttc
      301 gcggatgtcc aatgcaggcg caatgcgcac agcgcgaccc gtttttacat gcacaaacgt
      361 gacggcaact ttgccgtaat cttccacacg caatgtgcgg tgacctacgg tgcaatctgt
      421 cgcggcaatc acaccatcga caaaacaccc atctatttca gaaatgacca acatctgttt
      481 atgttgcggt ggttctgaaa acccgagcgc cttcatgcca gccaacccaa tacgcacacc
      541 taaaatttgc cttgggcaaa ggtgatcgtg gtctttcatg gatttttcga ggaggtgaga
      601 gatattcatg cgagcctttc gggcactcta tgagtaatta taactgccga atatattcca
      661 aatgtgcgca tagcatcaaa aaaacatccc tgaagtaaat gcttcaggga tgtaatattt
      721 ccttgcgatg atcttaccca caacctccgc cagatttatc gcgtttcaat tgcaacttaa
      781 tcttggcttc aaattctagg gcttcgtatt ccactgggtt tggcgagcag gattcgtagc
      841 ggctacccaa agcagagggg aagatataag ctaactcgtc atccccagca ttcgggttgg
      901 gttgagccgt gaaagtcgtg tcatccttgc caaagaaagc gatccagttg ttgactccgc
      961 cttccagaat gtagacattc gatacactgg atgcagagag aagcttccag gcttgaatgg
     1021 cagcggcttc gtcattgctc atgacgatga agactgaatt tgtgggtggc tcagagagca
     1081 acataggcac aatctctgtg atacgctcta gtggcacgtt gaccgcatct tcgatgtgat
     1141 acaagttgta atctgcttcg gaacgcacgt ccaaatagat cgggttcatc gcctgattgt
     1201 attttgcttt ataggcttca gcaggcgtaa tgaacaccaa acgcttttca agcatttggt
     1261 cggcggtgta gacataggta ttgataatcg ggtctgcatt caattgctcg actgtttcag
     1321 tgcgagtgaa ggttaactta ttgtagcggt ctgctaatga cggagcgcca atggcaacga
     1381 cagcaatagc tgctacgaga agtgcaccag ctccagcaag gcggatcttc ggctctttac
     1441 tcatatc
//
LOCUS       DAOREF010000863         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_86076_length_1442_cov_4.832733, whole genome shotgun sequence.
ACCESSION   DAOREF010000863 DAOREF010000000
VERSION     DAOREF010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86076_length_1442_cov_4.832733"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..>1442
                     /locus_tag="PKI33_15090"
     CDS             216..>1442
                     /locus_tag="PKI33_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38393.1"
                     /translation="MRPRWRKVFHDLWDNFMRTALVVLSIAVGVFSIGVITGAYEIIS
                     NDMGESYAANNPANIELRMTNFNDDVLSSIRNFKGVQDAEGRRVINYRVHPEGSTQWS
                     TIDLIAVEDFSKNNVNLLVPILGKAEAGKDEIVLNKKNMDKVHATVGDYLVFELADGT
                     TKKLKVVGVVQDSSTGADDFLAPPFAFTVMDTLQTLQQPDLFNRAYLTVSDSPNDLTH
                     IRTLGADLKDKLEKNGTTVIRTRYSERNKHPMTDTINAVLYILMALGVLIVFLSSSLI
                     ANTLSALLSQHLRHIGIIKLVGGRNKQVFAMYLTLIMAFAVLSLLIAIPLGGWGAYAL
                     AAFIADNLNFGLLGYRIVPIAFFVQVAVGLLVPLFAGLAPVLNGSRISVLRALSNDVA
                     GDESKPHVAGGKPRVNW"
BASE COUNT          355 a          280 c          370 g          437 t
ORIGIN      
        1 gatgtgttgg ctgctctttc caaggttggt ttggcgccga aaactgtgat cctcacctca
       61 gccatcaacc ccgagcgcgt gacccaatat ctgcgcgaag gtttgaaaga tgtaacggtc
      121 aagccgtatt ctgtaaacga attgaatgaa ctgttgaagt aaaggaaaga aagttgacag
      181 gtcttcaaga cctgtcaact ttgtaaaaga gaaacatgag accacgttgg cgtaaagtat
      241 ttcacgattt atgggataac ttcatgcgta ctgcattggt ggttttatcc attgcagtgg
      301 gtgtgttttc cattggggta atcactggtg cgtatgagat tatctcaaac gatatgggag
      361 aatcctacgc ggcaaataac cctgcgaata ttgaacttcg catgacgaat tttaatgacg
      421 atgtactgtc gtctattcgt aatttcaagg gtgttcaaga tgctgaaggc aggcgtgtga
      481 ttaactatcg cgtccatcct gagggcagta ctcaatggtc gaccattgac ttgattgcgg
      541 tagaagactt cagtaagaat aatgttaatt tattggtgcc cattttaggc aaggcagaag
      601 cgggcaagga tgagattgtc ttaaataaga agaacatgga taaagtccat gctacagtgg
      661 gtgattatct cgtctttgag ttggcagatg gaacgaccaa gaagttgaaa gtggtcggtg
      721 ttgtacaaga ttccagtacc ggcgctgacg attttcttgc ccctccattt gcgtttacgg
      781 tgatggatac acttcagacc ctccagcagc cagatttatt caaccgtgcc tatttgacgg
      841 tgagtgattc tcccaatgac ctcactcata tccgcacttt gggtgcagac ctgaaagata
      901 aactggaaaa gaacggtact accgttatcc gcactcgtta ttctgagcgc aacaaacatc
      961 ccatgacgga tacgatcaat gcggtcttgt atattttgat ggcattgggt gtgttgatcg
     1021 tgtttttatc cagctcgttg attgctaata cattgagtgc tttgttaagc cagcacttgc
     1081 gtcatattgg tattatcaag ttggtgggcg gtagaaataa gcaagtcttt gcgatgtatc
     1141 taacgctcat tatggcattc gctgtattgt ctcttctaat tgctattcca ttaggcggtt
     1201 ggggggctta tgcacttgca gcgtttattg cagataactt gaattttggt ttattgggtt
     1261 accgcattgt tccgattgca ttttttgttc aagtagccgt tgggttgttg gttccgctct
     1321 ttgctgggct tgccccggtt ctaaacggtt cgcgaatttc ggtgttgcgt gctttgagca
     1381 acgatgtcgc tggagatgaa agtaagccgc atgtggcggg cgggaaacct cgggttaatt
     1441 gg
//
LOCUS       DAOREF010000864         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_86241_length_1441_cov_1.922799, whole genome shotgun sequence.
ACCESSION   DAOREF010000864 DAOREF010000000
VERSION     DAOREF010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86241_length_1441_cov_1.922799"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1193
                     /locus_tag="PKI33_15095"
     CDS             210..1193
                     /locus_tag="PKI33_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM38394.1"
                     /translation="MNMAPIPHYFNIFPTGNIPVPVCIGDLPMSEYSVPQYTLTEEVA
                     SYLADNPLVPGVIVTRNGKISSIISRHKMFERLGHRFGVELFLRKHIVEMERELKAEA
                     FLLKSHITINMAVKLALSRTQGRIYDPIIVEFEDGTPFVLDMYVLLLSQSQLSQNLSG
                     IVSSLNTIELTLANEQANIASTLELVLESMEKVLPTHHLRIILKTEFDTAMLPHPSIL
                     YQNEPFQGNSVYRSVLVMNQPVVLEDVYNVPAWSNWDTPKNTRCWLGAPITNQFGTIG
                     LVSLARKTFSPFSNHEKEISLVFSRYLGKLFENAVRHAEKHRLLERKYYVP"
     gene            1245..>1441
                     /locus_tag="PKI33_15100"
     CDS             1245..>1441
                     /locus_tag="PKI33_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38395.1"
                     /translation="MPTINFNGKTYNNINEMPTNERQAFEKLSSLLVDANGNGIPDLL
                     EGDMVQNVMAIHATKTSINVNG"
BASE COUNT          422 a          335 c          302 g          382 t
ORIGIN      
        1 ggatcacaag gctcggttcc accttgaaag aagctcttaa aaggaacggg catcattaaa
       61 gaacacgagt aaaaatactc cgctcaatgt tgagcggagt attttttatt aacaccttac
      121 aagttctttt ctacaagctg catttaccag tcgttaatcc atagttcaaa caaggttaag
      181 tatgggtaca tataatcccc taaattaaca tgaacatggc accgattcct cattatttca
      241 atatctttcc aacaggcaac attccagtgc cagtttgcat cggtgacttg cccatgtctg
      301 agtacagcgt tccgcaatat acactgaccg aagaagttgc cagttatctt gcagacaacc
      361 cacttgtacc cggtgtgatt gtgacccgca atgggaaaat cagcagcatc atctcaaggc
      421 acaagatgtt cgaacgcctg ggtcaccgct ttggggttga attatttttg cggaaacaca
      481 tcgtagaaat ggaacgcgag ctcaaagcag aagcctttct actaaaaagc cacattacta
      541 ttaatatggc tgtaaaacta gccttaagcc gtacccaagg acgcatctac gatccgatca
      601 ttgtcgaatt tgaagatggc actccctttg tgctggatat gtatgtatta ctcttaagtc
      661 agtcacaact ctcgcaaaac ttgagcggca tcgtcagctc gctcaacacc attgaattga
      721 cgcttgccaa tgaacaggca aatattgctt ccaccttgga attagttctt gaaagtatgg
      781 aaaaggtgtt acccacacac catcttcgaa tcatcctcaa aacagagttt gatacagcca
      841 tgttgcctca tccttccata ctttatcaaa atgagccgtt tcaggggaac agcgtgtatc
      901 gctctgttct ggtgatgaat caaccggtcg tgttggaaga tgtctataac gtgcctgcct
      961 ggtcaaactg ggatacaccg aaaaacaccc gctgttggct tggtgctccg atcacgaatc
     1021 aatttggaac catcggcttg gtctctctgg cacggaaaac tttttctccg tttagcaatc
     1081 atgaaaaaga gatatcactg gttttctcgc gttatcttgg aaaattgttt gagaacgcgg
     1141 tccgtcatgc ggagaaacat cgcctgcttg aaagaaaata ttatgttccg taattctcag
     1201 ctcttctgcg tgtaaaatgg agggacaaga cgcaggagtt ctgtatgcct accattaatt
     1261 tcaacggaaa aacctacaac aatatcaatg agatgcccac caatgaacga caggcatttg
     1321 aaaagctatc cagcctgctt gtggatgcga acgggaacgg cattcccgat ctacttgaag
     1381 gtgacatggt gcagaatgtg atggcaattc atgccaccaa gacaagcatc aatgtcaacg
     1441 g
//
LOCUS       DAOREF010000865         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_86284_length_1440_cov_4.422383, whole genome shotgun sequence.
ACCESSION   DAOREF010000865 DAOREF010000000
VERSION     DAOREF010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86284_length_1440_cov_4.422383"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1261
                     /locus_tag="PKI33_15105"
     CDS             92..1261
                     /locus_tag="PKI33_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNM38396.1"
                     /translation="MTDLPIYPLTNEHKMLRDAARDFAQKEIAPIAAEFDESGDFPHS
                     TIKKMGELGFMGIEVPEQYGGAGMDPLAYVLALEEICKVDASHGVIMSVNNSLYCHGI
                     LKFGTEEQKKKFITPIASGQAIGAYSLTEPQSGSDAGTMRSRATRDGDFYILNGRKSW
                     VTSGPVANYFVVFMMTDPEKKQKGVTAFLVDGKTPGLTRGKKEPKLGIRASATSELIF
                     ENCRVPAENRLGAEGEGFKIAMTVLDAGRIGIATQALGIAEAAYEAARQYAGQREAFG
                     QPIGQFQGTGFKLADMKTRIEASRLLIYNAALAKEKAKESDGRYSLEASMAKLFASET
                     AMYVTHQAVQIHGGMGYSKELPVERYFRDAKITEIYEGTSEIQRLVISRLELGSK"
BASE COUNT          412 a          343 c          345 g          340 t
ORIGIN      
        1 tattatagtt aagagcaaga ttaaacgtca atcacaaatt tccgaataat acaaagggta
       61 taattcagaa ataccattcg gaggctttta gatgactgat cttcccatat acccacttac
      121 aaatgaacat aaaatgctcc gcgatgctgc ccgcgatttt gcgcaaaaag aaatcgcccc
      181 aattgcagcc gaattcgatg aaagcggaga ttttccacac agcaccatca aaaagatggg
      241 cgaattgggt ttcatgggta ttgaagtgcc agaacaatat ggaggcgcag gcatggaccc
      301 tctcgcctat gtcctcgccc tcgaagaaat ctgcaaagta gatgcctctc acggcgtgat
      361 tatgtcagtc aataattcct tgtattgcca tggcattcta aagtttggaa ccgaagaaca
      421 gaaaaagaag ttcatcaccc caatcgcttc tggacaagct atcggtgctt attctttgac
      481 agaaccccaa agcggctcag acgctgggac gatgcgcagc cgtgcgactc gcgatggtga
      541 tttctatatt ttgaatggac gcaaatcctg ggtcaccagc ggacctgtgg cgaattactt
      601 cgtcgtgttc atgatgaccg atcctgagaa aaaacaaaaa ggtgtaacag cctttctcgt
      661 agatggcaaa acacccggtc tgacacgcgg aaagaaggag ccaaaacttg gcatccgcgc
      721 ttcggcgacg agtgagttga ttttcgaaaa ttgccgtgtc cctgccgaga atcggctcgg
      781 tgcagaaggc gaaggcttca aaatcgccat gaccgtatta gacgctggtc gtattggaat
      841 tgcaacccaa gcgctcggga ttgcagaagc cgcctacgaa gccgcacgcc aatatgcagg
      901 tcaacgtgag gcgtttggtc aaccgatcgg gcaatttcaa gggacgggct ttaaactcgc
      961 agatatgaag acccgcatcg aagcatctcg tttattaatc tacaatgcgg ctctggcgaa
     1021 agaaaaagcg aaagagtcgg atggacgtta ttcccttgaa gcttcaatgg caaaactgtt
     1081 tgcttctgaa acagccatgt atgtaaccca tcaagctgtg caaattcatg gcggcatggg
     1141 ctatagcaaa gaattgccag tagagcgtta cttccgcgat gcaaagatca ccgaaattta
     1201 cgaaggcacc agcgaaattc aacgattggt tatttcgagg cttgaattag gctctaaata
     1261 acaattcatg aaagcgatcc ttttccacca acatggcgga ccggaagtcc ttgcatattc
     1321 cgacttccct actccgcaac ccaagcctgg cgaggcacta gttcgattaa gagctgcggc
     1381 tcttaatcgt gttgatgttt ttgtgcgcaa cggttggccc ggcttaaagc tggaacttcc
//
LOCUS       DAOREF010000866         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_86391_length_1439_cov_4.230491, whole genome shotgun sequence.
ACCESSION   DAOREF010000866 DAOREF010000000
VERSION     DAOREF010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86391_length_1439_cov_4.230491"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PKI33_15110"
     CDS             <1..833
                     /locus_tag="PKI33_15110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNM38397.1"
                     /translation="SKQMKSGENLRLRGADIRTGDAVLKRGRTLKPQDLGLLATLGHA
                     HVTVHKKARVALLSSGDELLDVSAPLEPGKIRDSNSYALAAAVSNLGVDVIRLGVARD
                     TRESITQLLDQAASENADLILSSAGVSVGAFDYIKEVIDANGKMDFWRVNMRPGKPLA
                     FGEYRGIPFIGLPGNPVSAFVGFEVFVRPVLERLNGKMDGGRQTVRVRCEEEIESDGR
                     ESYLRAKIQVKNDIRIAILTGHQGSGNLLSLVQADALLIIPAGVKCVPAGSEVDAILL
                     "
     gene            840..1265
                     /locus_tag="PKI33_15115"
     CDS             840..1265
                     /locus_tag="PKI33_15115"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNM38398.1"
                     /translation="MDKWLYRVMIALVIIGLAVSVYMTIYKISGNDGMCLGSGDCSTV
                     NASRFSEVNGIPVAVFGIVGYAAILIVLFYENKNEFFKRNGTLLAFGMSLTGFLFTLW
                     LVYVELVLLKAICPFCVTSQVSMTLIFIIAVVRLFRNEN"
BASE COUNT          352 a          321 c          383 g          383 t
ORIGIN      
        1 tctccaagca gatgaaatca ggagagaacc ttcgcctgcg aggcgcagat atccgcactg
       61 gagatgctgt gctgaaaagg ggtcgcactc tcaaaccgca ggacttgggt ctgctcgcca
      121 cacttggaca tgcacatgta acggtccaca agaaagcgcg agtggcattg ttatcctctg
      181 gagatgagtt attggatgta agcgctccgt tggaacccgg caagatccgt gactctaatt
      241 cctatgcctt ggcagccgca gtcagcaacc taggcgtaga tgtcattcgg ctgggtgttg
      301 caagagatac tcgcgaatct atcacccaac ttttggatca agccgcaagt gaaaacgcag
      361 accttatcct ctcctctgcc ggagtgagtg taggcgcctt tgactacatt aaagaagtga
      421 tcgacgccaa cggcaaaatg gatttttggc gggttaacat gcgcccaggc aaaccattgg
      481 catttggcga atatcgggga attccattca ttgggctgcc gggcaacccc gtttcagcct
      541 ttgttggctt cgaggttttt gtgcgccccg tgcttgaacg gctgaacggc aaaatggacg
      601 gaggcaggca aacggtccgc gtacgatgcg aagaagaaat cgaatcagat ggtcgcgaga
      661 gttatctgcg ggcaaaaatc caagtaaaaa atgacattcg tattgcaatt ttgaccggac
      721 accaaggctc gggtaacctt ttgtcactag tacaggcgga tgctttacta atcatccccg
      781 ctggtgtaaa atgcgtgcct gctggttcgg aagtagacgc tattctattg tgaggaacca
      841 tggataaatg gctttatcgc gtgatgatcg cgcttgtaat catcggtttg gcagtgtcag
      901 tttatatgac aatttataag atcagtggaa acgatggcat gtgcctcggc tctggtgatt
      961 gctccactgt gaatgccagt cgcttttcag aagtgaacgg aattcctgta gcagtatttg
     1021 gtattgtcgg ctatgctgcc atcttgatcg ttttgttcta cgagaacaaa aatgaatttt
     1081 tcaaaaggaa cggaaccttg cttgcctttg gcatgtcact tactggtttc ttgttcacgc
     1141 tttggttggt atatgttgaa cttgtattgc tcaaagcgat ctgcccgttc tgcgtcacct
     1201 cgcaagtctc tatgacgttg atctttatta ttgcggttgt acgactattc cgaaacgaga
     1261 attaggagga ttcgaaatgc cccgtattaa atgtcactat gttgattgtg tctttttgga
     1321 tgaaggattg tgcggcgcag cagccgtaga attcgaccca gacacaggtt gtgtgaccta
     1381 ttcccctgcc gatggcgccg cagcggaaga tgaatgggaa gatgaagaag agctcgacg
//
LOCUS       DAOREF010000867         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_86527_length_1438_cov_3.923355, whole genome shotgun sequence.
ACCESSION   DAOREF010000867 DAOREF010000000
VERSION     DAOREF010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86527_length_1438_cov_3.923355"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..>656)
                     /locus_tag="PKI33_15120"
     CDS             complement(280..>656)
                     /locus_tag="PKI33_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNM38399.1"
                     /translation="PRHYIIKEINMFPEITVTQLSEKLKSDEKFILLDVRELPELSQA
                     KIEDSRLEVTPMSRLGSEGPNALSESARSGEIPVYIMCHHGSRSAQVTMWLAQQGYKN
                     IFNVRGGIDAYARQVDSSVGFY"
     assembly_gap    657..680
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(698..>1438)
                     /gene="glgP"
                     /locus_tag="PKI33_15125"
     CDS             complement(698..>1438)
                     /gene="glgP"
                     /locus_tag="PKI33_15125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNM38400.1"
                     /translation="KERVRDRWTRGGFHPVQVIASGVLINPYALTIGFARRFATYKRA
                     SLVLSDVERLLELINRPNMPVQIIFAGKAHPADDPGKKLIQQVYRTVKKAETGGRIVF
                     IEDYEMNLARYLVQGVDVWMNTPRRPYEASGTSGMKAAINGVPNFSVMDGWWCEAYNG
                     KNGWKIGDEKKYASNEEQDAADAEDLYSTLEHQIIPKYYNRNPKDLMSPDWLAFMKDS
                     MKTVIPQFSTRRMVKEYVEKFYVPALKK"
BASE COUNT          329 a          353 c          365 g          367 t
ORIGIN      
        1 ttacaaatat gtgagaatcg aaaatcgccc tattgacatc caatttgatt tatgccatac
       61 ttttggtcac cagacggggg cacacaaatt gaatttgcca tccttatgaa aggaggaggt
      121 gatgtctatt atggatagta gtagtaaacc cagcgctgtg gcatccctcc tcaagtaagt
      181 caaaggaagc cacagcgccc cacgattgcc acccgcaagg gtggcagtct ggtttaatag
      241 agatgaataa aaaggtcacg cgaaaagcgt gacctttttt tagtaaaacc ctaccgaact
      301 atccacctgc cttgcgtagg catcgatccc tccgcgcaca ttgaagatat ttttatatcc
      361 ctgctgcgct aaccacatgg tgacctgtgc actgcggctg ccatgatgac acatgatgta
      421 gacggggatt tctcctgacc gtgccgactc tgataaagca tttggacctt ctgaacccag
      481 gcggctcatt ggcgtgacct caagcctgct gtcttcgatc ttggcttgag acaactctgg
      541 gagttcgcgc acatccaaca gaatgaactt ctcatccgat ttcagttttt cactgagttg
      601 ggtaacggta atttcaggga acatgtttat ttccttaata atgtagtggc ggggtannnn
      661 nnnnnnnnnn nnnnnnnnnn ccgccactac attgatatta tttcttcaac gccgggacgt
      721 agaacttctc tacatattcc ttcaccatac ggcgagtgga gaattgtggg atgacggtct
      781 tcatcgaatc tttcatgaag gcgagccaat ctggtgacat cagatctttg gggttgcggt
      841 tgtagtattt cggaatgatc tgatgttcga gtgtggagta caggtcttcc gcatcggcgg
      901 catcctgttc ttcgttcgag gcatatttct tttcgtcgcc gatcttccaa ccgttcttgc
      961 cgttgtaggc ttcgcaccac caaccgtcca taaccgagaa gttgggcacg ccgttgatgg
     1021 cggctttcat gccggaggtg ccgctcgctt cgtaggggcg gcgcggagta ttcatccaca
     1081 catccacacc ttggacgagg tagcgggcga ggttcatttc ataatcttca atgaagacga
     1141 tgcgaccacc agtctcagct ttcttgacag tgcgatagac ctgttgaatg agcttcttgc
     1201 cggggtcatc agctgggtgt gccttgcccg cgaagatgat ctgcaccggc atgttcgggc
     1261 ggttgatcag ttccagcaag cgttctacat ccgaaaggac gaggctggca cgtttatagg
     1321 tcgcgaaacg gcgggcgaag ccaatggtca acgcataagg gttgataagc acgcccgaag
     1381 cgatgacctg aaccgggtga aacccaccgc gagtccaacg gtcgcggacg cgttcttt
//
LOCUS       DAOREF010000868         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_86538_length_1438_cov_3.226320, whole genome shotgun sequence.
ACCESSION   DAOREF010000868 DAOREF010000000
VERSION     DAOREF010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86538_length_1438_cov_3.226320"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKI33_15130"
     CDS             complement(<1..636)
                     /locus_tag="PKI33_15130"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNM38401.1"
                     /translation="MKILPETALTYDDVLLVPQYSNVDSRRVLSTKSPLTKKIFLHSP
                     IVSANMDVVTESEMAITMAREGGIGIIHRFMTIAEQTRQIERVKKAESFVVENPLTMS
                     DQATVGEVKRVVEETDTGGILIVDKNGKLVGIITTRDLLFEEEDSKPVTSIMTREVRS
                     APTNTTLKDAERLLHEHRVEKLPLVDKDGKVTGLVTLKDIMKITKFPKATKD"
     gene            833..>1438
                     /locus_tag="PKI33_15135"
     CDS             833..>1438
                     /locus_tag="PKI33_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNM38402.1"
                     /translation="MTVIAVIGSQWGDEGKGKAVDFLAERADYVARFNGGNNAGHTVI
                     NEFGTFKIHLVPSGIFAQNTTGLIGGGVVVDPTVLIEEIEMLNKAGVNVNGRLWVSPR
                     SHIIMPYHKILDGLYEEAKGKGATGTTRRGIGPVFADKVSYNGIRWTDFTSDMFEQRL
                     EVQLTLKNKIITALGGEALDFKKVRDEYRGYYEKVKPYLKEL"
BASE COUNT          351 a          312 c          402 g          373 t
ORIGIN      
        1 gtctttggtg gctttcggaa atttggtgat cttcataata tctttcaagg tcaccaagcc
       61 ggttactttg ccatctttat ctacgagcgg aagtttctct acgcgatgct cgtgcaacag
      121 acgttcggca tcttttaggg tagtgtttgt gggcgcactg cgaacctcac gagtcataat
      181 cgaagtgaca ggtttgctat cctcttcttc gaataacaag tctcgtgtgg taatgatacc
      241 gaccaacttc ccattcttat cgacgatcag aatgccgcca gtatcggtct cttcgacaac
      301 acgcttcact tcgccgaccg tggcttggtc gctcatcgtg agcggattct ccacgacgaa
      361 ggattcggct tttttgacgc gctcgatctg gcgggtttgc tcggcaatgg tcatgaagcg
      421 atggatgatg ccaatgccgc cttcacgcgc catggtgatc gccatttcac tctcggtgac
      481 cacatccata ttggcggaga cgatgggcga atgaaggaat atttttttgg tgagcgggct
      541 tttggtggaa aggacgcggc gcgaatccac gttggaatat tgtggaacca acagcacgtc
      601 gtcatacgtt agggcggttt cgggcaaaat tttcatgcgt tctccaaact caggctttga
      661 aagttctgcg gctttaaggc gcggcacaaa ttataatgct tgatgttaga gcgtactcaa
      721 aactcgctga aacgctggca agcccgggcg gagcatgaag cgcggcgctt aacttggtca
      781 atcaaaaaaa atcgggtggt taaatcccga acatcgaata ttggaggttg tgatgacagt
      841 catagcagtg atcggttcgc aatggggcga tgaaggcaag ggcaaggcag tggattttct
      901 ggcagagcgt gcagattatg tggcgcgttt taatggtggc aacaatgccg ggcataccgt
      961 tattaatgag tttggaacgt tcaaaattca tttggtgcct tcgggcatct ttgcacagaa
     1021 tacaacgggt ttgatcggcg gcggtgtggt agtagacccc acggtgttga tcgaagaaat
     1081 tgagatgctt aacaaagccg gtgtcaacgt gaacggacgg ttatgggttt cgccgcgttc
     1141 gcacatcatc atgccatatc acaagatttt agatggcttg tatgaagaag ccaaaggcaa
     1201 aggtgccacc ggcaccaccc ggcgcggcat tggaccggtg tttgcagata aggttagcta
     1261 caacggcatt cgctggacgg attttacgag tgatatgttc gagcagcgtt tagaagtgca
     1321 attgaccttg aagaataaga tcattacggc gttgggcggt gaagcgttag acttcaaaaa
     1381 agtgcgcgat gaataccgcg gatattacga gaaggtcaag ccgtatctca aggaattg
//
LOCUS       DAOREF010000869         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_86769_length_1436_cov_3.547429, whole genome shotgun sequence.
ACCESSION   DAOREF010000869 DAOREF010000000
VERSION     DAOREF010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86769_length_1436_cov_3.547429"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKI33_15140"
     CDS             complement(<1..554)
                     /locus_tag="PKI33_15140"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HNM38403.1"
                     /translation="MSIFEIVYWAALVVEMVIRAPLNKKRKEEKISEQRVSQQEKLIL
                     FLLLIGMMILPLIYSATSWLDFANYNLPPWAGYLGALIMLSALFVFWRSHADLGLNWS
                     PSLEIRDKHELITHGIYGVIRHPMYASQWLWVLAQPLLLQNWLAGFINLLVFIPFYLL
                     RVQAEEKMMIDRFGEQYKTYMTKTG"
     gene            complement(551..805)
                     /locus_tag="PKI33_15145"
     CDS             complement(551..805)
                     /locus_tag="PKI33_15145"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNM38404.1"
                     /translation="MSERTYTEEFRVEGEKIIAKIKELLHEGNIRKVIIKDKDGKVLM
                     EIPVTIGVVGVLIAPQLAALGAIAALLTEATVIVEKTETL"
     gene            complement(818..1408)
                     /locus_tag="PKI33_15150"
     CDS             complement(818..1408)
                     /locus_tag="PKI33_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNM38405.1"
                     /translation="MPREYIGQNPTAFQRRPHLTKDDAWIRENINTFKAGHFATSVDG
                     QPYINTSTFWYDEAEHQIVFHSNLAGRIRSNIEYNPKVSFEASVLGQLLPSNVALEFS
                     LQYRSIVAFGTAQLVTDPAEMRRLLYGLIGKYFSELSVGQEYREINDKELRATSVYAI
                     KIEEWSGKENWEARADQSDEWQPLDEKWFEYYRNKS"
BASE COUNT          371 a          339 c          330 g          396 t
ORIGIN      
        1 cctgttttgg tcatatatgt tttgtattgt tcgccgaagc ggtctatcat cattttttct
       61 tctgcttgaa cacgcaagag atagaaggga atgaacacca gaaggttgat aaacccagcc
      121 agccaatttt gcagcagtaa aggttgtgca agcacccata accattgcga agcatacatg
      181 ggatgacgga tcacgccata aatgccatgg gtaatgagct catgcttatc gcgaatttcc
      241 aaggatggag accagttcaa gccgaggtct gcgtgagagc gccagaaaac gaataaagcg
      301 cttagcataa tcaatgcacc caaataacca gcccaaggcg gcaagttata gtttgcaaaa
      361 tccagccatg aagtagcgga gtaaatgagt gggagaatca tcataccaat gagaagcaag
      421 aagagaataa gcttttcctg ttgcgaaacg cgctgttcgc ttattttttc ttctttgcgt
      481 ttcttattaa gcggggcacg aatgaccatc tccacgacca aagccgccca ataaacgatt
      541 tcgaaaatac tcatagggtc tctgtttttt caacgatcac agttgcttct gtgagcaagg
      601 cagcaatggc gcctaatgcg gcaagctgtg gggcgatcaa cacaccgaca acaccgatcg
      661 tgacggggat ttccattaag accttgccat ctttatcttt gatgataact ttgcgaatat
      721 ttccctcgtg tagcaattcc ttgatcttgg cgatgatttt ttcgccttcc acacgaaact
      781 cttcggtata ggttctttca ctcatgcgtt tatcctttca ggatttgttc ctgtaatact
      841 cgaaccattt ttcatcgagc ggctgccatt catcgctttg atcggcacgt gcttcccaat
      901 tttctttgcc gctccattct tcgattttga tggcatacac cgacgtggct cgcagttctt
      961 tgtcgttgat ctcgcgatat tcctgtccaa cggacagttc agaaaaatat ttgccgatca
     1021 acccatataa cagtctacgc atttcagccg gatcggttac aagctgggcg gttccaaaag
     1081 cgacgatgct gcgatactgg agcgaaaact ccagcgcgac attcgaaggc agtaattgac
     1141 caagaacact cgcctcaaaa ctgactttgg ggttgtactc aatattcgag cggattctcc
     1201 ccgccagatt ggagtgaaac acgatctgat gctccgcttc gtcgtaccag aaagtggagg
     1261 tgttgatata aggctgccca tcaacagagg tggcgaaatg acctgctttg aaggtgttga
     1321 tgttctcgcg aatccacgca tcgtctttgg tgaggtgcgg tctgcgctga aaagctgtgg
     1381 gattctgacc aatatattct cttggcatga tgttctccta ttgattgtct acgagc
//
LOCUS       DAOREF010000870         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_87277_length_1432_cov_2.693537, whole genome shotgun sequence.
ACCESSION   DAOREF010000870 DAOREF010000000
VERSION     DAOREF010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87277_length_1432_cov_2.693537"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKI33_15155"
     CDS             complement(<1..203)
                     /locus_tag="PKI33_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38406.1"
                     /translation="MSAENDIIALRSRVAELERKLDFLYRRMGIEYVDDPGMIDSQII
                     SLLKKGNKLEAIKVYRELTNTDLA"
     gene            complement(269..898)
                     /locus_tag="PKI33_15160"
     CDS             complement(269..898)
                     /locus_tag="PKI33_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38407.1"
                     /translation="MKNQTPLLLTVSLILILVASCTPKATQDPNLLIQQAVNATLAAI
                     PTATQVSPPTPYPSPTPFSLAGLFCEYQFCIGHPIDVSFFDVSAQQNPASPSTYGQGI
                     LAALNGNLFIQVMWQLAPGTSDPKFLFDTMLNDSIDTATNTSSIFLVRDMNVFYDDIT
                     TLATPSLPYGGAGAWTCGDRVFAWKVYTPQSGTARGMFDEMLARFTCGQ"
     gene            complement(954..>1432)
                     /locus_tag="PKI33_15165"
     CDS             complement(954..>1432)
                     /locus_tag="PKI33_15165"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNM38408.1"
                     /translation="YVMQGEIYTAGAFGDQGHQMSDTTKLLGEEPEYFVFNGAVNALK
                     DKKPLKANVGESIRIFFGVGGPNFTSSFHVIGEIFDRVYDQASLTSAPLTNVQTTLVP
                     PGGATMVEFNVEVPGNYVLVDHALSRLQRGLAGVLIVEGDQNPEIYNGVPSAGSGH"
BASE COUNT          341 a          306 c          412 g          373 t
ORIGIN      
        1 gccaaatcgg tattggtcaa ttcacggtag accttgatgg cttcaagttt gttacctttt
       61 ttgagcaggg aaatgatctg tgaatcgatc atgccggggt catcaacata ttcaattccc
      121 attcttctat aaagaaaatc gagctttctt tcaagttcag cgacgcgtga gcgcagtgca
      181 ataatatcgt tttcagcaga catagcttat ctccttcaaa acgatattac acccgctcgt
      241 tcttgctgac attcacctat atttcctatt actgtccgca ggtgaagcga gctagcattt
      301 catcgaacat gccacgagcg gtgcctgatt gaggggtgta taccttccag gcgaagacac
      361 ggtcgccgca ggtccaggcg ccagcgccgc catatggtag ggacggcgtt gccagagtgg
      421 taatgtcatc gtagaacacg ttcatatcgc ggactagaaa aatgctggag gtgtttgtgg
      481 cggtatcaat gctatcgttt agcatcgtat cgaacagaaa cttagggtct gaggtgccag
      541 gggcaagttg ccacattact tgaatgaata gattgccgtt gagcgcagcc aatatgcctt
      601 gcccataggt gctaggagat gctgggtttt gttgggcgct tacatcgaaa aaggaaacat
      661 cgatgggatg accgatgcag aattgatatt cgcagaataa gccagccaaa ctaaaaggtg
      721 tgggcgaggg ataaggtgtg ggtggcgaca cttgtgttgc agtgggaatg gcggcaagag
      781 ttgcgttgac cgcttgttgg atgagcaggt ttgggtcttg agtcgctttc ggtgtgcagg
      841 aagcgacgag aatcagaatc aggctcaccg ttaagagaag aggcgtttgg tttttcatcc
      901 gcgaaattat aaaggaaaag ccccgtctaa tttcgacggg gctttggttt gctctagtgt
      961 ccgctccctg cggatggaac accgttataa atttctgggt tttgatcgcc ttcgacgatg
     1021 agtactccgg cgagaccgcg ttgtaatctg gataaggcgt ggtctacaag gacgtagttg
     1081 ccaggcactt cgacattgaa ctcaaccatg gtggcgcctc cgggcggaac gagggtggtt
     1141 tgcacgttgg tgagcggcgc actggtgaga gaggcttgat cgtagacgcg gtcgaagatt
     1201 tcaccgatga cgtggaaaga tgatgtgaag ttcggtccgc cgacaccgaa gaagatgcga
     1261 atgctttcgc cgacattggc tttgagcggc ttcttgtctt tgagggcatt gacggcacca
     1321 ttgaaaacga agtattcagg ttcttcaccg agcagtttgg tggtatcact catttggtga
     1381 ccttgatcgc cgaatgcgcc agcggtgtag atttcgcctt gcattacgta ga
//
LOCUS       DAOREF010000871         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_87515_length_1430_cov_2.991273, whole genome shotgun sequence.
ACCESSION   DAOREF010000871 DAOREF010000000
VERSION     DAOREF010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87515_length_1430_cov_2.991273"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKI33_15170"
     CDS             <1..752
                     /locus_tag="PKI33_15170"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNM38409.1"
                     /translation="GSLEQIGFLPDMGWDHSDLYINMYGGFWYNEDGTQMTVNSQPVL
                     DALTWQQQFYTKYGVDNVMAFSSGWADTYMSADYPFYTGKLAMYVDGEWMTGPNFINA
                     FKPEASYGVAPFPPPADHPDRANTAVVQGTVAVIPSGAANKEASAKLLAWMMSPEVVA
                     EEFCFNANLPTSKKAAEDPCFTSNPKFKVFTDLISSPNAKIIVTTPISLEFNDAWGLV
                     IEQVLHAGMDPKPILDDLQAEFEVKLADALK"
     gene            830..>1430
                     /locus_tag="PKI33_15175"
     CDS             830..>1430
                     /locus_tag="PKI33_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNM38410.1"
                     /translation="MEELRTTGWKFSWPPKFKKWGMNERRSFKIGMLFLLPWTIGFLA
                     FTLYPMLASLVYSFTIYHPRQPLEWNGIKNYTDLLTDDLFWTALYNTLYMVLIGVPLT
                     LFSSFFCAILLNLKVRGQAYYRVIYFLPSIVPTVASTILWLWILNPQAGIMNTLLSYI
                     GIDGPNWMTNPAWSKPGLILLGMWGMGNTIIIYLSGLQDV"
BASE COUNT          354 a          331 c          344 g          401 t
ORIGIN      
        1 atggatctct tgaacagatc ggtttcttgc ctgatatggg ctgggatcat agcgacctgt
       61 atatcaatat gtatggtggc ttctggtaca acgaagacgg aactcagatg accgtcaaca
      121 gtcaacccgt gttggatgct ctcacttggc aacaacagtt ctataccaaa tacggtgtcg
      181 ataatgtgat ggcgttctca tctggctggg ctgatacata tatgtctgcc gattatccct
      241 tctatacagg caaacttgcc atgtatgtgg atggtgaatg gatgacgggt ccgaacttca
      301 tcaatgcctt caagcccgaa gccagctatg gtgttgcgcc cttcccgcca ccagcggatc
      361 atcctgatcg cgcaaatact gccgtagttc aaggaaccgt agcagtaatc cccagtggtg
      421 ctgcgaataa agaagcctct gccaagttgc tggcatggat gatgtctcct gaagttgtgg
      481 cagaagaatt ctgcttcaat gcgaacctgc cgaccagcaa gaaggcagct gaagacccat
      541 gcttcacaag caaccccaag ttcaaggtat tcacggattt gatctcaagc ccgaatgcca
      601 agatcattgt cactactcct atttcgcttg aatttaacga tgcctgggga ttggtgatcg
      661 aacaagtttt acatgcgggg atggacccca aaccaatttt ggatgatttg caagctgagt
      721 ttgaagtgaa actggcggat gcactgaaat agcctttgcc ataaattttg ggcggagcgt
      781 acatacgctc cgcccatact gatatttcgt agccaattgg agataagaca tggaagaatt
      841 gcgaacaaca ggatggaagt tttcctggcc tcccaaattt aagaaatggg gcatgaatga
      901 acgccgcagt ttcaagatcg gaatgttgtt tctgcttccg tggacaattg gttttttagc
      961 cttcactctt tatcccatgc tggcatctct tgtgtacagc tttaccattt accatccacg
     1021 gcagccttta gaatggaatg gcatcaaaaa ttacactgac ttgctgaccg atgacctctt
     1081 ttggacagcg ctctataaca ccttgtatat ggtgctgatc ggcgttccgt tgacgttgtt
     1141 ctcatccttt ttttgtgcaa ttctcttgaa cctaaaagtg cgcggacaag cctattaccg
     1201 cgtgatttat tttttgccgt ctattgtgcc gaccgttgcc agcaccattt tgtggttgtg
     1261 gattttgaat ccgcaagcag ggatcatgaa caccttgctt agctatattg gcattgacgg
     1321 accaaactgg atgaccaacc cggcatggtc caagccgggg cttattttgc taggtatgtg
     1381 ggggatgggg aatacgatca tcatttatct ttcaggcttg caagacgtgc
//
LOCUS       DAOREF010000872         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_87620_length_1429_cov_3.673945, whole genome shotgun sequence.
ACCESSION   DAOREF010000872 DAOREF010000000
VERSION     DAOREF010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87620_length_1429_cov_3.673945"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1429)
                     /locus_tag="PKI33_15180"
     CDS             complement(<1..>1429)
                     /locus_tag="PKI33_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNM38411.1"
                     /translation="DTPVINLIAPESREMIIHNIQDRDKGLAVPSDYETVALRKDGST
                     FILDVRISTYILQGEQYTLVILRDITERKRAEEALQIKEWAIRSSINALALSDLDGSL
                     IYVNPAFLALWGYQTEQEVLGRSAIEFWQVQENADQVISALRTQGGWMGEMVATRKDG
                     TLLDVEVVASMVADNAGKPLCMMGAFVDITDRKRAEVELTIAKEKAEENEKKLREVLD
                     NTKVQLWAFDGTRYNYVSKEWYDFTGQTLDTPLTIDLWVSAVHPDDREPSGKIWLENW
                     ERKTAHDNYFRLRRQDGVYRDFYCHAIPIFDSQNKFKYFHGFNVDITERKQAEEKLRH
                     AENRLELFFSQSLDGFFFMLLDEPIQWSDDIDKDKTLEHVFAHQRITKANDAMLSLFG
                     TTRKQFIGLTPKDLYPGDVEQRKHFWGDLFDKGHLQLLTAETKSDGSIMWLEGDYVCM
                     YDTDGRLLGHFGIVRDVTERKQAEAV"
BASE COUNT          365 a          365 c          299 g          400 t
ORIGIN      
        1 aaccgcctct gcctgcttgc gttcggtgac atcgcgtacg atgccaaaat gccccaaaag
       61 acgtccatcg gtatcataca tgcacacata atccccttca agccacatga tggaaccatc
      121 agacttggtc tcagcggtaa gcagctgaag atgtccttta tcaaaaagat caccccaaaa
      181 atgcttcctt tgttcgacat cgccgggata aagatctttc ggtgtcagcc caatgaactg
      241 cttacgagtt gttccaaaca gagacaacat ggcatcgttt gcttttgtga tgcgctgatg
      301 agcgaaaaca tgctccaatg ttttatcttt atcgatatca tcactccatt gaatcggctc
      361 gtctaaaagc atgaagaaaa agccatcaag agactgtgag aaaaaaagtt caaggcgatt
      421 ttccgcatgg cgcaatttct cctccgcttg tttgcgttca gtgatatcca cattaaagcc
      481 atgaaagtat ttgaatttat tttggctatc gaaaatcggt atggcatggc aataaaagtc
      541 cctatatacg ccatcttgtc ttctcaatcg aaaataatta tcgtgcgcgg ttttcctttc
      601 ccaattttcc aaccatattt tccctgaagg ttcgcggtca tcaggatgta ctgccgaaac
      661 ccacagatca atggtaagcg gggtgtctag tgtttgcccg gtgaaatcgt accattcctt
      721 gctcacgtag ttatacctgg ttccatcaaa agcccacaat tgaactttgg tgttgtccaa
      781 cacctcgcgt aacttttttt cattttcttc tgccttctct ttggcaatgg ttaattccac
      841 ttccgcccgc ttacggtccg tgatgtcgac aaatgcaccc atcatgcaaa gcggtttgcc
      901 ggcattgtct gcaaccatgc tggctaccac ttcaacatca agcaatgtgc catcttttcg
      961 cgtggctacc atctcgccca tccagccgcc ttgagttcgt aatgctgaga tgacttgatc
     1021 cgcattttct tgtacctgcc agaactctat agccgacctg cctagaacct cctgctcggt
     1081 ctggtatccc cacaaagcaa gaaaagcggg gttgacgtat atcaggctcc catccaagtc
     1141 agatagggca agggcattaa tggaagaccg gatagcccac tctttgattt gcaacgcctc
     1201 ctctgcccgc ttgcgctcgg tgatgtcgcg cagaatgact agtgtatatt gttcgccttg
     1261 caatatgtag gtggaaatac gcacatccaa aataaaagtt gatccgtctt ttcggagcgc
     1321 gacggtttca taatcagaag gcactgctaa acctttatca cgatcttgaa tattatggat
     1381 gatcatctcg cggctttcag gcgcgatgag attgataacg ggagtgtca
//
LOCUS       DAOREF010000873         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_87945_length_1426_cov_4.938731, whole genome shotgun sequence.
ACCESSION   DAOREF010000873 DAOREF010000000
VERSION     DAOREF010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87945_length_1426_cov_4.938731"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..451
                     /locus_tag="PKI33_15185"
     CDS             89..451
                     /locus_tag="PKI33_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM38412.1"
                     /translation="MSKRNIVHVEIPAVNTAAAGEFYQELFGWKTTPMPEMNYVMWEA
                     GSGDEYGGFPEVSAENPAGQVLVYIASDDIDEDLEKVSQLGGKVLREKTEIPGMGWYG
                     VFQDPTGNVLALYTGMNS"
     gene            464..748
                     /locus_tag="PKI33_15190"
     CDS             464..748
                     /locus_tag="PKI33_15190"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNM38413.1"
                     /translation="MTGTLTAQIGKRELLVNILVRASALVMTMPGCKAYIVLEDLKDE
                     RTVAVFEMWDDKEAHDVSLQDPRVRSLISEAMPILAGAPSGGEYRVATNL"
     gene            799..>1426
                     /locus_tag="PKI33_15195"
     CDS             799..>1426
                     /locus_tag="PKI33_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38414.1"
                     /translation="MSGVRVLVGTRKGAFILTSDAKRKKWKVSGPHFAGWEIYHMHGS
                     SVDPNRLYASQSSGWFGQVIQRSNDGGESWEPVGNQFAYHGTPGTHMWFDGTQHPWEF
                     KRVWRLEPSLTDVDTVYAGVEDAALFRSTDGGQTWTELAGLRDVKGHLWQPGAGGMCL
                     HTIVLDPTDPKRMFIAISAAGAFRTENGGETWLPINQGLSSGELPDADA"
BASE COUNT          362 a          311 c          411 g          342 t
ORIGIN      
        1 ggaagcgaaa aacaattcta gaggagggct tgaaaattag aaaaaatgtt ctataataga
       61 tagtaaatat cgaattaagg agaatgtcat gtccaaacga aatattgtcc atgttgagat
      121 tcctgctgtg aatactgcgg cggcaggcga gttctatcaa gaacttttcg gctggaagac
      181 cacgcccatg cccgaaatga actatgtgat gtgggaagcc ggcagtggag atgaatatgg
      241 tggcttccct gaagtttctg ccgagaaccc tgctgggcag gtgctggtct acattgccag
      301 tgacgatatt gacgaagatt tggaaaaggt cagccaattg ggcggcaaag tactgcgcga
      361 aaagaccgag attccgggca tgggttggta tggggtgttc caagacccaa ctgggaatgt
      421 tctggcttta tatactggga tgaattctta atttatgtac gctatgaccg gaaccctcac
      481 ggctcaaatt gggaagcgtg agcttttagt gaacatcctt gtgcgtgctt cggcattggt
      541 tatgaccatg ccgggctgca aagcctatat tgtgctggaa gacctaaaag acgagcgaac
      601 cgttgccgta tttgaaatgt gggacgataa agaggctcac gatgtatctt tacaagaccc
      661 tcgtgtaaga agcctaatct ctgaggcaat gcccatcctt gccggggcac cgagtggtgg
      721 cgaataccgt gtagcaacaa acctgtaact gaaagagcaa gcttaagtcg ttactattta
      781 ttgaccaagg agagcgtgat gagcggagta cgagtattag ttggaacccg taaaggggca
      841 tttattttga cctcagatgc caaacgtaaa aaatggaagg tgagcggtcc gcattttgca
      901 ggctgggaga tctaccacat gcacggctct tctgtggacc cgaaccgctt gtatgcttcg
      961 cagtctagcg gttggtttgg gcaggtcatt cagcgttcaa atgatggcgg tgaatcgtgg
     1021 gagccggttg gaaatcaatt tgcatatcat ggcacgcccg gcacccacat gtggttcgat
     1081 ggcacacaac acccatggga atttaaacgg gtgtggcgct tggaaccctc tttgaccgac
     1141 gtggacaccg tctacgccgg ggtggaagat gcagctttat tccgctccac tgatggcggg
     1201 cagacgtgga ccgagttagc cggactgcga gacgtgaaag gtcatctctg gcaacccggc
     1261 gcaggtggaa tgtgcctgca caccattgtg cttgacccga ctgatccaaa gcgtatgttt
     1321 atcgctattt ctgccgcagg ggctttccgc actgaaaatg gcggcgagac gtggttgccg
     1381 atcaatcagg gattgtcttc cggcgaattg cccgatgccg atgcgg
//
LOCUS       DAOREF010000874         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_87949_length_1426_cov_3.997812, whole genome shotgun sequence.
ACCESSION   DAOREF010000874 DAOREF010000000
VERSION     DAOREF010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87949_length_1426_cov_3.997812"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKI33_15200"
     CDS             <1..342
                     /locus_tag="PKI33_15200"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM38415.1"
                     /translation="PLGNSDKAYECQVLSAEPHDPLLVSASFVAKWLVANIGKSTWDS
                     DNADYRYIDGYRFHLQSAYDFPVSVAPGVTVELTVDMQAPPTPGQYSTFWIIYIGKNA
                     FCKMQLDITVQ"
     gene            complement(400..777)
                     /locus_tag="PKI33_15205"
     CDS             complement(400..777)
                     /locus_tag="PKI33_15205"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNM38416.1"
                     /translation="MTKFIIRWLINAVALYAAVWLIPGIDYLGDWKGILALALIMGLL
                     NALVRPLLKFLTCPLIILTLGLFTLVINTVMLMLTSSIGQSFGIPLMVDGFWSALLGS
                     LVMSVVSIVMSVIFRDELKGKRD"
     gene            complement(811..>1426)
                     /locus_tag="PKI33_15210"
     CDS             complement(811..>1426)
                     /locus_tag="PKI33_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38417.1"
                     /translation="RALTLRAMQHAHEKKVNDIWLHVRADNPEAIHLYETLGFVERAR
                     RTAWQAYTDANTSQVETDIAITNRYPRFWGTQLKWLSRLYPDALSWHMNWSFSSLKPG
                     LFNWLYLMFLDMNIRQWAATRKDDLLATLSWIPTGRGESLFAATGERSDPEALRALLT
                     QARRDLSRSYPHISLEFPAGLFDDAIQAAGFKALRTLIWMQATS"
BASE COUNT          391 a          320 c          343 g          372 t
ORIGIN      
        1 ccgcttggta actctgataa agcatatgaa tgtcaggttc tttcggctga gccgcatgac
       61 ccgcttcttg tctcagccag ttttgttgct aaatggcttg tagctaatat aggaaagtct
      121 acatgggact ctgataatgc agactatcgc tatatagacg gatatcgctt tcatttacaa
      181 tctgcatatg attttcctgt aagtgttgct ccaggagtta cagttgaatt gacagttgat
      241 atgcaagcgc ctcctacccc aggacagtat tccacttttt ggattatcta cattggtaaa
      301 aatgctttct gtaaaatgca attagatatt acagtgcagt aaaacacata acaaaaagag
      361 cccttacttg taagggctct ttttgttatg tgtttttttt taatctcgtt tacctttgag
      421 ttcgtcgcgg aatatgacgc tcatcacaat gcttaccacg ctcattacca aactgccaag
      481 caaggcagac cagaacccat ccaccatgag cggaatgcca aacgattgac cgatagaact
      541 ggtcaacatc aacatgacgg tattaatgac gagggtgaac aagcccaaag taagaataat
      601 aagtgggcag gtgaggaact tgagcaatgg acgcacaagc gcattcaata accccataat
      661 aagggcgagg gcaagaatac ctttccagtc gccgagatag tcaataccag ggatcagcca
      721 cacggcagca tataacgcaa cagcgttgat cagccagcga ataataaact tggtcatttt
      781 ttctcctttc ttttatgtac gcaaaatatt ttacgaagtt gcctgcatcc agatcaatgt
      841 gcgaagcgct ttgaatcctg cggcttgaat agcatcgtca aagagaccag cgggaaattc
      901 aagcgagata tgagggtagg aacgagacag gtcgcggcgt gcttgagtga gaagcgccct
      961 caacgcctct gggtcggacc tctcgcctgt tgccgcgaat aggctttctc cacgccccgt
     1021 cggaatccac gagagcgtgg cgagcaagtc atctttgcga gtagccgccc attgacggat
     1081 attcatatcg aggaacatca ggtacaacca gttgaacaaa cccggtttga gcgaggaaaa
     1141 actccagttc atgtgccaac ttagggcgtc gggatagagg cgggaaagcc atttgagttg
     1201 tgttccccaa aagcgtggat atctattcgt gatggcgata tccgtttcca cttgtgaagt
     1261 gttggcatct gtataggctt gccatgcggt tcggcgggct cgttcgacaa atccaagtgt
     1321 ttcgtagagg tgaatggctt cagggttatc agcacggacg tgtaaccaaa tatcattcac
     1381 ctttttttca tgagcatgtt gcatggcgcg cagagtgaga gcacgt
//
LOCUS       DAOREF010000875         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_88088_length_1425_cov_3.816788, whole genome shotgun sequence.
ACCESSION   DAOREF010000875 DAOREF010000000
VERSION     DAOREF010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88088_length_1425_cov_3.816788"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKI33_15215"
     CDS             complement(<1..289)
                     /locus_tag="PKI33_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNM38418.1"
                     /translation="MAQNKFNRRDFIKLSAVGIAAAAGTRLLTEEAKASEGVKGEHQW
                     VMVIDQSKCTGCNQCNMACHATNDINPEMEWTKVLDMGEMNGKQVYLPRPCM"
     gene            complement(276..1268)
                     /locus_tag="PKI33_15220"
     CDS             complement(276..1268)
                     /locus_tag="PKI33_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNM38419.1"
                     /translation="MFSRFEKIILALMVSLLIAGVSILAASAQDANPPTPTGDEEETC
                     ADCHEEFEMSWKNGPHGNATSDPIFVKDWTDQGKPSACLACHVTGYNENTGAWEKDGV
                     YCTACHLDDGGDHPKTTMSVDATGGTCGTCHTDARFGMKEWEESTHFAAGMDCTTCHD
                     PHNASLKITVNLKEQKVADASQLCISCHEEASMNFPYSTHSKQGVTCIDCHLEHVESD
                     AAVHQVADHSFKASITTCNSCHANQMHTDGQAEATEQAIVASVAPTETPKPAAATVDQ
                     SPIVSPDPAPVSPLGYAGITALIGIAAGMLLAPWLERGYRLVLKKSAEVHHGSK"
BASE COUNT          316 a          340 c          387 g          382 t
ORIGIN      
        1 gcatacatgg gcgcggcaga tagacttgct tgccattcat ttcgcccata tccagcacct
       61 tggtccattc catctctggg ttgatgtcgt tggtggcatg gcaagccata ttgcattgat
      121 tacagcccgt gcacttggat tggtcaatga ccattaccca ttgatgctcg ccttttacac
      181 cttctgaggc ttttgcttct tcagtgagca gacgcgttcc cgcagcagcg gcaatgccta
      241 ctgcagaaag cttgataaag tcacggcggt tgaacttatt ttgagccatg gtgtacctca
      301 gcagatttct tcaatacgag gcggtatccg cgttctagcc agggagccag caacatacca
      361 gcggcaatgc cgatcagtgc tgtgatgcct gcatacccga gcggagaaac aggagcgggg
      421 tctggtgaaa cgataggaga ttgatctacc gtagcggcgg caggtttggg tgtttctgtt
      481 ggggcaacgc tcgcaacaat agcttgttcc gtcgcttcag cctgaccatc tgtatgcatt
      541 tggttggcat ggcaagagtt acaggttgta atgcttgctt tgaacgagtg gtctgccact
      601 tggtgaactg ctgcatcact ttccacatgc tcaagatgac aatcgataca agtgacgcct
      661 tgtttgctgt gggtggagta tgggaagttc atgctggctt cttcgtggca gctaatgcac
      721 aactgagaag catctgccac cttttgttct ttgaggttga cggtgatctt caaagaagca
      781 ttgtgtgggt catggcaggt tgtgcagtcc atgccggcag cgaagtgagt gctttcttcc
      841 cattctttca taccaaaacg agcatcggtg tgacaagtac cgcaggtacc gccggtcgca
      901 tccacactca tggtggtctt ggggtggtcg ccaccatcgt ctaaatgaca agctgtgcaa
      961 tacacgccat ctttttccca agcgccagtg ttttcgttgt acccggtcac atggcaagcg
     1021 agacaagcac ttggtttgcc ttggtcggtc caatctttga cgaagatcgg gtcgctggtg
     1081 gcatttccat gcggaccgtt cttccagctc atttcaaatt cttcatggca gtctgcgcag
     1141 gtttcttctt catcccctgt gggcgtaggg ggattggcat cttgtgcgga agcagcgaga
     1201 atggaaacac ctgcaatcag cagggaaacc ataagggcga ggatgatttt ttcaaaacgt
     1261 gagaacatga gagcatctcc tattaagagt gaaacttaag agttaagcgt gagctttcga
     1321 tggctttagc accggtgtag gcgcattgat aaagatttcg caaccgaggc aggcttcacg
     1381 catatagcca tttgaaagac ggtggatctg ccagcagggt tttcc
//
LOCUS       DAOREF010000876         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_88237_length_1424_cov_3.487217, whole genome shotgun sequence.
ACCESSION   DAOREF010000876 DAOREF010000000
VERSION     DAOREF010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88237_length_1424_cov_3.487217"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKI33_15225"
     CDS             <1..427
                     /locus_tag="PKI33_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNM38420.1"
                     /translation="LMKIGTMLKDVIASLFKKNSTQLYPFEQTPTPEHFRGSLYYDPS
                     ACTGCCLCVKDCPAKAIELVTLDRAAKRFVLKYHMDRCIYCGQCAVNCRFKCMGMSNT
                     DWEHASLNKKEFTVYYGKDEDIETLLAGITTPNAQPDTE"
     gene            354..902
                     /locus_tag="PKI33_15230"
     CDS             354..902
                     /locus_tag="PKI33_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HNM38421.1"
                     /translation="MEKTKILKPSWQESLRQTLSQIQNENGDLPRVAIVGIGNPLRND
                     DIAGTLVANELLQRECAIDANHFLVIQTGHAPENVTGELRDFRPHLIILIDAADMRAE
                     PGTVCWVPIQLIEGKSASTHSLPLSMLARYLTLELNCAVVLVGIQPKSTAIGETASPE
                     ILAAVNEVVEEISTNCLTVRTH"
     gene            1086..>1424
                     /locus_tag="PKI33_15235"
     CDS             1086..>1424
                     /locus_tag="PKI33_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38422.1"
                     /translation="MELNSLSLKQFRNLFVSLPVPDMTSIRGVYRATFVGPSWLRTSA
                     GPALALSGLGGWWGKEFSTDGTAINIVLRTGKFFTRFPMKLVAAQSFIDGKDGLALHY
                     QPGNPFPWMYV"
BASE COUNT          378 a          368 c          316 g          362 t
ORIGIN      
        1 tctcatgaaa atcggaacga tgctcaaaga tgtcattgca tcgctgttca agaaaaacag
       61 cacacagctc tatcctttcg aacaaacgcc tactccagag cacttccgtg gcagtctata
      121 ttatgaccct tccgcctgca cgggttgctg tctctgtgta aaagattgcc ctgccaaagc
      181 catcgaactt gtcacgctcg accgcgccgc caaacgcttc gtcctcaaat accacatgga
      241 ccgttgcatc tattgcggtc aatgtgcggt taattgtcgc ttcaaatgta tgggcatgtc
      301 caatacagat tgggaacatg cctcactcaa taaaaaggag tttacggttt attatggaaa
      361 agacgaagat attgaaaccc tcctggcagg aatcactacg ccaaacgctc agccagatac
      421 agaatgaaaa cggagatctt ccacgagtcg ccattgtagg gattggaaat ccgcttcgca
      481 atgacgatat tgccggcaca ctcgtagcaa acgagttgtt acagcgcgag tgtgcaatag
      541 atgcaaatca ttttcttgtt atccaaacag ggcacgcccc cgaaaatgtc accggcgaac
      601 tccgcgattt tcgcccacac ttaatcatcc tgatcgatgc cgccgatatg cgcgcagaac
      661 ctggtacggt ttgttgggtg cctattcaat tgattgaagg caagagcgcc tctacccata
      721 gcttgcctct ctccatgctg gcgcgctatc tgaccctcga actcaactgt gccgttgtac
      781 tggttggcat tcagccaaaa tccactgcaa taggagagac cgctagccca gaaattcttg
      841 cagctgtgaa tgaagttgta gaagaaataa gtacaaattg cctcaccgtt cgaacacact
      901 aaaacatctc tgaaatcctt agggtttaag atgtttggta ttaagaaggg ggaatcacta
      961 tatatggatt catttcccga aaaatcatgc cgtataatta acaggctgag gaacacctcg
     1021 caaaagccca gtctactttt tgatggtata tcttttccct gctaacaggc agaatggtca
     1081 aagttatgga actcaattcc ctctccctca aacaattccg caatctattc gtatcgctac
     1141 ccgtgccaga tatgacatcc attcgtggtg tatatcgcgc cacgtttgtg ggaccaagct
     1201 ggttacgaac ctccgccggt ccggcattgg cgttgtctgg gctgggtggc tggtggggca
     1261 aagaattcag cacagatggc acagccatca atatcgtttt gcgcacagga aaatttttca
     1321 ctcgctttcc tatgaagctg gtagctgccc aatcattcat cgatggtaag gatggtctcg
     1381 ccttgcatta ccagcccggc aaccccttcc cgtggatgta tgta
//
LOCUS       DAOREF010000877         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_88304_length_1424_cov_2.054785, whole genome shotgun sequence.
ACCESSION   DAOREF010000877 DAOREF010000000
VERSION     DAOREF010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88304_length_1424_cov_2.054785"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKI33_15240"
     CDS             complement(<1..239)
                     /locus_tag="PKI33_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNM38423.1"
                     /translation="MKWDVFISHAFEDKAYTRQLATALHRKGLKVWFDEFELKTGDGL
                     TRTIDHGLSESKYGIVVLSPNFFAKEWTQKELGALT"
     gene            complement(477..710)
                     /locus_tag="PKI33_15245"
     CDS             complement(477..710)
                     /locus_tag="PKI33_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38424.1"
                     /translation="MKTWEYLHALADNHEVVEVNGAMIGTALRGFVTTYTGRPTLTDF
                     LNQSGQEGWELVGMSPVGEVGGALRLIFKRPTN"
     gene            complement(865..>1424)
                     /locus_tag="PKI33_15250"
     CDS             complement(865..>1424)
                     /locus_tag="PKI33_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNM38425.1"
                     /translation="WLPTEKSVRVALRTQQIIAHESGVADSIDPLAGSYLIEHLTDEI
                     EKGALEYIAKIDEMGGAMQSIERGFMQNEIQNAAYAAQQAIEKKEQIIVGVNQFTMDE
                     VLTLERLKVDTSIELGQREKLKKLREQRDPNVVEQLRGRLVEAAKGSENLMPLFIECV
                     EQDMTLGEICNTLRGVWGEYVAEGF"
BASE COUNT          322 a          388 c          279 g          435 t
ORIGIN      
        1 gttaatgccc ctagttcttt ttgtgtccat tcttttgcaa aaaaattggg gcttaagact
       61 acaatcccat atttagactc tgataatccg tgatctatgg ttcgtgttaa gccatcgcca
      121 gtttttaatt caaactcatc aaaccacact ttcaaacctt ttctatgtaa ggctgttgcc
      181 agttgccttg tgtaagcttt atcctcaaaa gcatggctaa taaatacatc ccatttcatt
      241 ttgcctccta cataaaactt tttttcttaa aattatatac aaaaatgggt aacttttgtc
      301 gcccattgac acttaaaaac ccttccgttt catattcgct cttccactct gaagagtatt
      361 tcgtaaaaat ctcacaattt atactatctc ctacattttc ttcatcctag ggtttgggag
      421 aagaagtcat ggtctaaaac agagacagtc accccaaggt gactgtctct gttgctttaa
      481 ttcgtaggtc tcttgaaaat gagccgtaac gcgcccccaa cttcgcctac gggactcatc
      541 ccaaccagct cccagccttc ctgcccagat tggttgagaa agtcggtcag tgtgggtctg
      601 ccggtgtacg tggtgacaaa tcctctcagt gccgtgccga tcatggctcc attgacttca
      661 acgacctcgt gattgtccgc gagtgcatgc aaatattccc aagtcttcat gttctttcgt
      721 tccttaccat ttcctttttg attttttctc tcctccccta aattcgacgg cacttctgtc
      781 gaatttgggg gacgtgtgcc ctttagggta ggtgcctgcg atagcacatc gtcccgatgt
      841 tcttcgggag gcggaggggg cgggctaaaa cccttccgcc acgtactcac cccaaacgcc
      901 tctcagcgtg ttacaaatct cgccgagggt catgtcctgt tccacacatt caatgaagag
      961 cggcatcaag ttctcgctgc cttttgcggc ttccaccaac cgtccgcgca actgttcaac
     1021 gacgttcggg tctctctgct cgcgtaactt cttcaacttc tcgcgctgtc ccaactcaat
     1081 cgacgtatca accttcaaac gttccaaggt gagaacttca tccatggtga attgattgac
     1141 accgacaata atttgttctt tcttctcgat ggcttgctgc gccgcatagg ctgcgttttg
     1201 gatttcattt tgcataaatc cacgctcaat ggattgcatc gctccgccca tttcgtcgat
     1261 cttggcgatg tattccagcg cacctttttc aatttcatcg gtcagatgtt caatcagata
     1321 tgaacccgcc aacgggtcaa tcgagtccgc gacaccggat tcgtgcgcga tgatctgttg
     1381 agtccgcaac gccacacgta ccgatttttc tgttggcagc caca
//
LOCUS       DAOREF010000878         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_88359_length_1423_cov_3.535819, whole genome shotgun sequence.
ACCESSION   DAOREF010000878 DAOREF010000000
VERSION     DAOREF010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88359_length_1423_cov_3.535819"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PKI33_15255"
     CDS             complement(<1..570)
                     /locus_tag="PKI33_15255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM38426.1"
                     /translation="MITDTVWKLPDIVNRYLTYRAAIPLAQEQIGVMMSILKTREQPI
                     ENFLDLGCGDGILGASILGSYPKARGVFVDFSEPMLTQARDVLQDYDGQLAFENLDYG
                     NPTWVTSMQTYAPFDAIVSGYSIHHQPDERKREVYKEIFSLLKPGGWFVNVEHVSSAN
                     EVNVNLFEKHYTSVRHAIETQNGGTRSLQE"
     gene            complement(590..>1423)
                     /locus_tag="PKI33_15260"
     CDS             complement(590..>1423)
                     /locus_tag="PKI33_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNM38427.1"
                     /translation="IGTGGGTGHVFEYTGSAIRSLTMEQRMTICNMSIEGGARAGMIA
                     PDEVTFEYLKGREFAPQGADWDKAVSYWKTLATDEGATYDKSITLNADEIEPMITYGT
                     NPGMGMGITDRIPTVESFSEPSQKAAFEKAMAYMGLQPGQSLLGQKVDVVFIGSCTNS
                     RISDLRLAASVIKGKKVASGIRVMVVPGSQDVKKQAEQEGLDKIFKEAGAEWREAGCS
                     MCIAMNGDQLQPGQYAISTSNRNFEGRQGKGGRTFLASPVTAAVTAIHGVVTDPRTVL
                     S"
BASE COUNT          325 a          357 c          380 g          361 t
ORIGIN      
        1 ctcttgcaag ctgcgtgtgc cgccgttctg tgtttcgatg gcatgccgaa cggatgtgta
       61 atgtttctcg aacaggttca cattgacttc gttcgccgat gaaacatgct ccacatttac
      121 gaaccatccg ccaggtttta gcaagctgaa gatttcctta tacacttcac gcttacgttc
      181 atcgggttga tgatgaatgg agtagcctga aacaatggca tcgaaaggcg cgtaggtttg
      241 catagaggta acccaggttg ggttgccata gtcgagattt tcaaacgcaa gttgaccatc
      301 atagtcttgc aaaacatcac gtgcctgtgt gagcatcggc tcggagaaat ctacgaacac
      361 gccgcgggct ttggggtaac tacccagaat ggatgcacca aggataccgt caccgcagcc
      421 gagatcaaga aaattctcaa tgggctgttc tctggttttg agaattgaca tcatcacacc
      481 gatctgttct tgcgccaatg gaatggcagc acgataggtc aggtaacgat tgacgatgtc
      541 ggggagtttc caaacggtgt cggtgatcat ggtgtttaca ggctgttctt taagacaata
      601 ccgttcttgg atccgtaaca acaccgtgaa tggctgtcac tgcagcggtg acagggctgg
      661 ctaagaatgt acgtccacct ttgccttggc gaccttcaaa attacggttg ctggtggaga
      721 tggcatattg cccaggctgg agttgatcgc cattcatcgc gatacacatt gaacagcctg
      781 cctcacgcca ttcggcaccg gcttctttga agatcttatc gagaccttcc tgctcggctt
      841 gcttctttac atcctgcgag ccgggcacga ccatcacacg gataccactt gcaaccttct
      901 tgcctttgat gacagaagcc gcaagacgca gatcagagat gcgggagttg gtgcaggagc
      961 cgatgaagac cacatccact ttttgaccga gcagagattg acccggttga agccccatgt
     1021 acgccatggc tttttcgaat gcggctttct gtgaaggttc gctgaatgat tccacggtgg
     1081 gaatacggtc ggtgataccc atgcccatgc cgggattggt tccgtaggtg atcatcggtt
     1141 cgatctcatc ggcattgagc gtaatagact tatcgtaggt agcgccttcg tcggtggcga
     1201 gagtcttcca gtaggagacg gctttgtccc agtctgcgcc ttgaggagca aactcgcgtc
     1261 ctttgagata ttcaaaggtc acttcatctg gagcgatcat accggcgcgc gcgccgcctt
     1321 caatggacat gttgcagatg gtcatgcgct gttccatggt gagtgaacgg atggcagagc
     1381 cggtatattc aaagacgtga cccgttcccc cacccgtgcc aat
//
LOCUS       DAOREF010000879         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_88467_length_1422_cov_3.686174, whole genome shotgun sequence.
ACCESSION   DAOREF010000879 DAOREF010000000
VERSION     DAOREF010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88467_length_1422_cov_3.686174"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..855
                     /locus_tag="PKI33_15265"
     CDS             181..855
                     /locus_tag="PKI33_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNM38428.1"
                     /translation="MTNAQITIREKKLGLLIRDARVAERRSIKECADAIGVKAGVFRA
                     YEEGRRAPSLPELEALVFFLKIPISQFWGNETISDAPEPLKPEDITKLIALRHRMIGA
                     LIRQERTNANMSIRHLSSETGIPQSRLKAYELGERTVSVPELESILAVMGSRIENFFD
                     QSGPVGEWMTTQLAMQKFMDLPNEFQEFVCQPVNRPYLELAMKLSSMSRDKLRSVAEG
                     LLDITL"
     gene            861..1382
                     /locus_tag="PKI33_15270"
     CDS             861..1382
                     /locus_tag="PKI33_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38429.1"
                     /translation="MSLEPHQLDAKGKQAFAKKNFVEAAEYFRQAAEGYTQGRAGLEA
                     AESRNNLSVALLQAGKPQEALDAALDTDKVFEGAKDVKRQAMAFGNQAAALEGLHRYD
                     EAIQKYELSAELFGSIDEGDLRAMVMKSAAAIKLKTGKITDSAFKMMGSLDVKKNPTF
                     FERIMKFLMKFIR"
BASE COUNT          384 a          333 c          341 g          364 t
ORIGIN      
        1 caagacggtc aagttttggg cgagttacag cctacgggta ttcgccccaa cttcacccca
       61 aggttagagg cggctggttt caagttgggt gcagaggtgt ttatgacgtc taaacaacag
      121 caacagtcgt caagacgttg acgcattctg ctataataag ggcttatttg atgaggtaac
      181 atgaccaacg ctcaaattac tattcgcgaa aagaaacttg gtttgttgat tcgagatgcc
      241 cgtgtggcag aacgtcgcag tatcaaggag tgcgccgatg cgattggcgt aaaagcaggt
      301 gtgttccgtg cctatgaaga aggacggcgc gcaccctctt tgcctgaact ggaagctctt
      361 gtattctttt taaagattcc catttcgcaa ttttggggca atgaaactat ttcagatgct
      421 cccgagccct taaaacctga agatattacc aagcttattg ctttgcgcca tcgcatgatc
      481 ggcgcgctga tccgtcagga acgcacgaat gcgaatatgt ccattcgtca tctttcatcc
      541 gagaccggaa ttccacagtc acgattgaag gcatatgaat tgggcgaacg tacggtttct
      601 gtgcccgagc tggaaagcat tcttgctgtt atgggcagcc gtatagaaaa tttcttcgac
      661 caaagcggtc ctgtgggcga gtggatgaca actcagcttg ccatgcaaaa atttatggac
      721 ctccccaatg aatttcaaga atttgtttgc cagcctgtga accgcccgta tcttgaactc
      781 gccatgaagc ttagcagcat gtctcgcgat aaacttcgct ctgttgcaga aggcttgctc
      841 gacatcaccc tttaataatt atgtctcttg aacctcatca actcgacgcc aaagggaaac
      901 aagcctttgc caaaaagaac tttgttgaag ctgctgaata ttttcgccag gcagctgaag
      961 ggtacaccca aggacgtgcc gggcttgaag cagcagagtc tcgtaacaac ttaagtgttg
     1021 cactcttgca agcaggcaaa cctcaggaag cactcgatgc agctcttgat accgataaag
     1081 tctttgaagg tgccaaagat gtgaaacgtc aagccatggc gtttggtaac caagctgcgg
     1141 cgctcgaagg tcttcatcga tatgatgaag cgattcaaaa atacgaactc tctgcagaat
     1201 tgtttggcag tattgatgaa ggtgacctgc gcgcaatggt catgaaatct gcggctgcca
     1261 tcaaactcaa aacaggcaag atcaccgatt ctgctttcaa aatgatgggc tcgctcgacg
     1321 taaaaaagaa tccaaccttt tttgaacgta tcatgaagtt tctcatgaaa tttatcagat
     1381 gatcaatctt cacctgcata tccggcgcgc cgttgcgcag ga
//
LOCUS       DAOREF010000880         1421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_88595_length_1421_cov_3.106881, whole genome shotgun sequence.
ACCESSION   DAOREF010000880 DAOREF010000000
VERSION     DAOREF010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88595_length_1421_cov_3.106881"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..488
                     /locus_tag="PKI33_15275"
     CDS             156..488
                     /locus_tag="PKI33_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38430.1"
                     /translation="MSRKRKFILLFLITVIATTPIFARAKIKYFSAYGACQNRSMSIK
                     GNKTWEYQNTTNRNLISTVSFSDGYNKLECDAIGVGPFWVVILTVSGCRGNTIEICPP
                     NEDDYSID"
     gene            836..>1421
                     /locus_tag="PKI33_15280"
     CDS             836..>1421
                     /locus_tag="PKI33_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38431.1"
                     /translation="MGKEKFLPIVFSSGLIFSLVFTVCIMTGWFIGSSIGQSNPEIFV
                     ATKFPWDTPAMLSAQAAGQGGLSGGLIGLILGLFLSFGSFGWFVAQLWKRKYPKMEVR
                     DSQMLVFGWMFLPLRGGKLPESILDKFNPIVPKAIHEKIIKRSAKGFLIGCIVGGIPM
                     CLGISTALGTPFVNLSYDWAVLMAIGFSLMGITGT"
BASE COUNT          411 a          261 c          309 g          440 t
ORIGIN      
        1 agttttacac gccttatcat ttttctggct ggacggcttc gccatccccg ccccaacgca
       61 ggtaacgcaa accgttaggc aacctctcgt aaaaataaag ttacacttga attagagtct
      121 tggttcaagt atatttattt caaataataa aacttatgtc tagaaaacga aaatttatac
      181 tactgttcct gataacggtt attgccacaa cgcctatttt tgcccgtgca aaaattaaat
      241 atttttctgc ttacggagca tgtcaaaaca ggtctatgtc aataaaaggt aataaaacat
      301 gggaatatca gaatacaact aatagaaatt taatatcaac agtaagtttc tctgatggat
      361 acaataaatt agaatgtgac gccatcggtg ttggaccatt ttgggttgta atacttaccg
      421 tgagcgggtg tcgtggtaat acaattgaaa tatgccctcc taatgaagat gattactcta
      481 tagattgagt atttttggca aagtcagatt ttcaaaaagc aaggttttgg acgcacaagc
      541 gttttggtta gtcatgcttt tttaggctgt tgccaaaatc aaagttatat gtaaaagtta
      601 ggtatattag cggtggtgtc tttttttcta caagcgtttc ggtcagcggg caggttgcct
      661 aacacagcgt gcacctgacg tgtgggattc tgcgcgattt ataagcattt ttctggcttc
      721 gagtttttcc tgctcccaaa gcgaatccac gcccgcccac acgcaggtaa cgcaaaccgt
      781 tgggcagata gcattacaag gaaatcaaag attgctttat gcataggaga ttcgaatggg
      841 taaagaaaaa ttccttccta ttgtattttc atcagggtta atctttagcc ttgtttttac
      901 cgtatgcatt atgacaggat ggtttattgg cagtagcata ggtcagtcta atccagagat
      961 ttttgtggct actaaattcc cttgggatac ccctgctatg ttatctgctc aggcagcagg
     1021 acaaggtgga ctaagcgggg ggctaatagg attaattctc ggcttattcc tgagttttgg
     1081 tagttttggt tggtttgtcg ctcaattatg gaaaagaaaa taccccaaaa tggaagtcag
     1141 agattcgcaa atgttagttt ttgggtggat gtttctccca ttgcgtggag gaaaattacc
     1201 tgagagcatt ttggataaat tcaacccaat agtgccaaaa gccatccatg agaaaatcat
     1261 aaaaagaagt gcaaaaggat ttctgatcgg atgcatcgtg ggtggtattc caatgtgttt
     1321 aggaataagc accgctctag gtaccccatt cgtaaacctg tcatatgatt gggcagttct
     1381 tatggcaata ggcttttctc tcatgggcat aactggtaca t
//
LOCUS       DAOREF010000881         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_89862_length_1411_cov_3.444690, whole genome shotgun sequence.
ACCESSION   DAOREF010000881 DAOREF010000000
VERSION     DAOREF010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89862_length_1411_cov_3.444690"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKI33_15285"
     CDS             complement(<1..441)
                     /locus_tag="PKI33_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38432.1"
                     /translation="MSKDITRQVANALSVVLALTVNILASTLPLNGQNTGEISDRFLV
                     YFVPAGYVFAIWGIIYIGWIAFTIYQFQPAQKENPRLQNLGYFFALSGLFNAAWLVCW
                     HYNWFGLSVLVMLTLLALLIASYLKLNVGLTPVNTAEKWSVDIPF"
     gene            complement(563..>1411)
                     /locus_tag="PKI33_15290"
     CDS             complement(563..>1411)
                     /locus_tag="PKI33_15290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM38433.1"
                     /translation="ARAEADTNVDSGITLSSYRNLKLIGRGGMAEVYRATDSTGRGTV
                     AVKILRAALAGDEQFQKRFMREAEIVNRLEHPNIVRVTDFGEERGMYYIVMEMLSGPD
                     LNHLIRQEKRISLSNTVSLLRGIADALDYAHINGLVHRDIKPSNVMLDSSTIPSRSVL
                     TDFGIAKMLDAHTRITNTAGMLGTFDYMAPEQIQASADVDGRADIYALGVMTYQMLTG
                     RLPFERPDTGAILLAHLNAPPPDARELIHDLPRSAARAVQKAMAKDVRDRFASAREFI
                     GALETD"
BASE COUNT          384 a          337 c          342 g          348 t
ORIGIN      
        1 gaagggaata tccacgctcc atttttcagc agtattgaca ggtgtaagtc ctacattaag
       61 tttgagataa ctggcgatga gcagggcaag caaagtgagc ataaccaaaa cactcaagcc
      121 gaaccagttg taatgccagc acaccaacca tgccgcgttg aaaagtccgc ttaaggcaaa
      181 gaagtagccc aggttttgca agcgtggatt ttctttttgt gcgggttgaa attgataaat
      241 ggtgaaggcg atccaaccaa tgtaaataat gccccaaatt gcaaagacat acccagcagg
      301 gacgaaatat acaagaaagc gatctgagat ttcgccggtg ttctgtccgt taaggggcaa
      361 agtgctggca agaatgttga cggttaatgc aagaacgact gaaagtgcat tggctacttg
      421 acgagttata tctttagaca tgtctctctc cattattgat gtactgagat ggataggact
      481 acagctatgt ctcttactgt caaatactat cagagcggca gaaaagaaaa acatgcttgg
      541 ggagcatgtt ttataaaagt ctttaatcgg tttcgagggc gccaataaat tctcttgcag
      601 aggcaaaccg gtctcgcaca tcctttgcca ttgccttttg cacagcacga gccgccgaac
      661 gtggtaggtc gtgaattaac tcgcgcgcat cgggcggcgg tgcatttaag tgcgccagca
      721 aaatggcacc agtgtcgggt cgttcgaatg gcaagcgccc ggtcagcatt tggtaggtca
      781 tcacacccaa agcgtaaata tctgcgcgtc catctacatc ggcagacgct tgaatttgtt
      841 ctggcgccat ataatcaaag gtgccaagca tgcccgctgt attggtaatg cgagtatgag
      901 cgtccagcat cttggcaatg ccaaaatcgg tcaacaccga acgtgaaggg atagtggaag
      961 aatccaacat tacattcgac ggcttgatat cacggtgaac caatccgttg atgtgggcat
     1021 aatccaacgc atcggcgatt cctcgcaaca ggctcacagt gttggataaa gaaatgcgct
     1081 tctcttgccg gatcagatgg ttgaggtctg gacccgaaag catttccatc acgatgtaat
     1141 acattccacg ttcttcgcca aaatcggtca ctcgcacgat gtttggatgt tcaagtctat
     1201 tgacgatctc cgcctcacgc atgaaccgtt tctgaaattg ttcatcaccc gccaatgcgg
     1261 ctcgcaggat tttgaccgct actgttcctc gccctgttga atctgttgcg cgatagactt
     1321 ctgccatgcc gccccgcccg atcaacttga gattacgata cgaagacaaa gtaatgccgc
     1381 tatccacatt cgtatcagct tctgcacggg c
//
LOCUS       DAOREF010000882         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_89981_length_1410_cov_4.136531, whole genome shotgun sequence.
ACCESSION   DAOREF010000882 DAOREF010000000
VERSION     DAOREF010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89981_length_1410_cov_4.136531"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..1329)
                     /locus_tag="PKI33_15295"
     CDS             complement(472..1329)
                     /locus_tag="PKI33_15295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM38434.1"
                     /translation="MAVQTKNQIEYKTIRLSNGRKVNYVHQGTGEPVVMVHGLAASLH
                     DWDDLLPEVSAAGYAGYALDLLGHGESEKPSHTHDYSVENAYADFSAWIDSLNISFPI
                     NLIGHSLGGGLSLLYTYRNPEKVKSLVLVNPFYSMKQLPQILQKMFHHQLINTTLIDR
                     TPYRVFRFFVDMTSFSTYIGSRETHVLPEHIRYQTALDYKRAASGIYNMPRTLHHLDL
                     DLARITQPTLLLWGGRDQTLAPSSFPQLAEKLPNLVKKHSFPICGHVPHQCHPADFNP
                     YVMEFLDTH"
BASE COUNT          382 a          343 c          337 g          348 t
ORIGIN      
        1 ggtggcgagg atcgggcgct cataactgct gaaacgctgg cggcatttca ggcactcacg
       61 ccgacggcga atcccgccgt gactgtcgtg tgaggtgtcc agcaccttcg agtcatgatg
      121 tttacaatac ggacaacgca tccggtagaa gctccaaatt agaacaaatg atttagccat
      181 atatgggcgt aaaagcgctg catccccgta tatctggcta atgtgttcgg cattttagca
      241 ccgaggtcaa ttgtgtgcaa gagggtattt gaaaactgca atcttaaaat attgactcga
      301 ttccccatta ggcgctagat gatggacaaa aatccgcgct tcattcttaa cgttgaattg
      361 attctcccgc tgcaaacatt cctacactga acgttttttc tcagaaaaac aaaaacccga
      421 agagcaaaaa gtatatttgc tcttcggaat tatttactca atcgtccgcg gttagtgtgt
      481 atccaagaac tccatcacat acgggttgaa atccgctggg tgacattgat gcggcacatg
      541 tccacagatc gggaaggaat gtttcttcac caagttcggc aacttctctg ccaactgcgg
      601 gaaagaagac ggggcaagcg tttggtcacg tccgccccac agcagcagag tcggctgggt
      661 gatgcgcgcc aagtccaggt caaggtgatg aagcgtgcgc ggcatattgt aaatgcccga
      721 agccgcgcgt ttgtaatcca gcgcggtctg ataacgaata tgttccggca acacgtgcgt
      781 ctcgcggctg ccaatatagg tgctaaagct ggtcatatct acaaaaaagc ggaagacgcg
      841 atacggcgta cggtcgatca aggtcgtgtt gatcaattga tgatggaaca tcttctgcag
      901 aatctgcggc aactgtttca tcgaatagaa cgggttgacc aacaccaaag acttaacctt
      961 ctcagggttg cgataggtgt ataacagcga aagcccgcca cccaacgaat gcccaatcaa
     1021 attaatcggg aaggaaatat tcagggagtc gatccatgct gagaaatctg cataggcgtt
     1081 ttccactgaa taatcgtgcg tatggcttgg cttttcgctt tcgccatgcc caagaagatc
     1141 cagtgcatac ccggcataac ccgccgcaga aacttctggc agcaggtcat cccaatcatg
     1201 caaagatgcc gccaacccat gcaccataac gacaggttcc cctgtcccct gatgtacata
     1261 attcactttg cgtccatttg acaaacgtat ggttttatat tcgatttgat tcttcgtctg
     1321 tactgccatg tgtatctcac aaagatgccg catgtgtagg acgcggcatc tgtaaaacat
     1381 cttaattcaa ttgtattaat atcgggtaaa
//
LOCUS       DAOREF010000883         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_90500_length_1406_cov_5.745374, whole genome shotgun sequence.
ACCESSION   DAOREF010000883 DAOREF010000000
VERSION     DAOREF010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90500_length_1406_cov_5.745374"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..781
                     /locus_tag="PKI33_15300"
     CDS             62..781
                     /locus_tag="PKI33_15300"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNM38435.1"
                     /translation="MTELLYQTDAYLKEFSAVITSVDAEARAVVLDRSAFYPGGGGQP
                     CDFGTLEVNGVKYPVEKVKKQGDDVLHVLGGTEPLPTPGSASLGTLDWVRRHKLMRTH
                     TALHILCGTVFRDYGAKVTGGDMEPLKGRMDFEFETMRAELVREIEAAVNAEVKKNHE
                     IRVKILPREEAFQIPDLIRTKINLLPEGIMQVRTVEIVGLDLQADGGTHVANTSEVGT
                     IKVVEYKSKGAINKRLYIEIE"
     gene            878..1288
                     /locus_tag="PKI33_15305"
     CDS             878..1288
                     /locus_tag="PKI33_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38436.1"
                     /translation="MKAKKLSILLAAIMLVAVQLACAAGEPTFSNVRTATDQDGTQLS
                     TTFGSFDTVYVVGDLSNGVVGNNVTARLFVDQVDGFDPNFFVGEAAVDVDQDNINSVY
                     FYFEPPTDGWPVGTYKVEVYFNGTLNTTLSYSIQ"
BASE COUNT          350 a          332 c          355 g          369 t
ORIGIN      
        1 agccctctaa tccaatccta attttgtctg accaattcat tccatcttgt ataattgagc
       61 catgaccgaa ctcctctacc aaacggatgc ctatctcaaa gagttttcag ccgtcatcac
      121 atctgtcgat gctgaagctc gtgctgtcgt tctcgaccgt tctgcttttt accctggcgg
      181 gggaggtcag ccctgcgatt ttggcacgct ggaggtcaat ggcgtgaaat acccagtgga
      241 aaaagttaag aagcagggcg atgacgtcct tcacgttctc ggtggaaccg aacctttgcc
      301 aacgcccggc tccgcctctc tcgggacctt agactgggtg cggcgacata agctgatgcg
      361 cacccatacc gctctgcaca tcttatgcgg aacggtcttc cgcgattatg gtgccaaggt
      421 tactggtggt gatatggagc cgctcaaagg gcgtatggac tttgaatttg agaccatgcg
      481 cgcagaactc gtccgcgaga ttgaggcggc ggttaatgcc gaggtgaaga aaaaccatga
      541 gatcagagtc aagatcctcc cgagggaaga ggcgtttcaa atcccagatt tgattcgcac
      601 caagatcaac ctcctcccag aaggaatcat gcaagtgcgc accgtcgaga ttgtaggctt
      661 agatttgcaa gccgacggcg gtacgcatgt agcaaacacc tctgaagttg gaaccatcaa
      721 agtggttgaa tataagagta aaggcgcgat caataagcgc ctttatattg aaattgaata
      781 gcccttttag gggagggaag ttcgtaatag tttaggtata atgtcgacac ttggcggggc
      841 tacctgccta catttatatt atgaggagaa tgccaaaatg aaagcgaaaa aactgtcgat
      901 cttgctggct gcaattatgt tagttgctgt tcagctcgcc tgcgccgctg gcgaacctac
      961 gttctcgaac gtccgcacgg caaccgatca ggatggtacc caactatcca ccaccttcgg
     1021 gtcttttgac accgtttatg ttgtaggtga tctttccaat ggcgtggttg gtaacaatgt
     1081 tactgctcgt ttgttcgtcg atcaggtaga tggttttgac ccgaacttct ttgtgggtga
     1141 agctgctgtg gatgtggatc aggataatat taactcagtc tacttctact tcgaaccgcc
     1201 taccgatggt tggccagtcg gtacctataa agtggaagtg tatttcaacg gcacgttgaa
     1261 taccactctc agctattcga ttcaataaag tttttcgtgt aagccaagcg gctgcctgaa
     1321 aaggcagccg cttttgtttt gatgtaccct cggtttataa tacagtggaa ggtgagcgaa
     1381 gtcgattaga attccccaaa aaccaa
//
LOCUS       DAOREF010000884         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_90504_length_1406_cov_4.517395, whole genome shotgun sequence.
ACCESSION   DAOREF010000884 DAOREF010000000
VERSION     DAOREF010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90504_length_1406_cov_4.517395"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKI33_15310"
     CDS             <1..511
                     /locus_tag="PKI33_15310"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNM38437.1"
                     /translation="AWVHENIQGYGGDPNRIALVGHSAGADIVSNVVVNPNYLREYGL
                     ELSNIICAAPLDTEGFDKTKADENEQKMWQNALSNNQNYLTETSATLLIHPNIGIPPM
                     LGVTRGNERRQQIATEFITTLQEAGIEATLIDATSLTHEEVNNQIGAAGDTVMTAPLM
                     SFLTECFER"
     gene            840..1352
                     /locus_tag="PKI33_15315"
     CDS             840..1352
                     /locus_tag="PKI33_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38438.1"
                     /translation="MSKSDLSPEHILLRNGALLIIPPLVITFGLWGALPVAYSPDVFW
                     KDIPEWLGVFENVFRIFVFSIPGMLYFGKAEKGQPLGWYLYFGGLLIYLASYGAQIVH
                     PGSLWSQSIIGFTAPAWSTVFWFAGIGLVCARSWLPIPWCRAIYLVIASCFLIFHIWH
                     TGLVYFSITQ"
BASE COUNT          403 a          292 c          291 g          420 t
ORIGIN      
        1 cgcatgggtt catgagaata ttcaaggcta cggtggcgac ccgaaccgca tcgctttagt
       61 cggtcactca gcaggtgcag acattgtatc caatgtagta gttaatccaa attacttaag
      121 agaatacgga ttggaactgt ccaacatcat ttgcgccgct ccgcttgata cagaaggttt
      181 tgataagaca aaggcggatg aaaacgaaca gaagatgtgg caaaacgcgc taagtaataa
      241 ccaaaattat ctaaccgaaa catctgccac tctgcttatc catccgaata ttggtattcc
      301 acctatgctg ggggtaacac gcggaaacga acgtcgccaa caaatagcaa ctgaatttat
      361 cacaacttta caagaggctg gtattgaagc cacacttata gacgccacat ctcttacaca
      421 cgaagaagtt aacaaccaaa tcggcgcagc aggcgatacg gtgatgactg caccgttaat
      481 gagtttttta acggaatgtt ttgaaaggtg aattttttat ttggcttgta tctcgcagca
      541 tagtgaagtt aaggtaaaac tcgagtcaat aaagcatccc gaaaaacatc gggtaatgca
      601 cctaatctct tctgtccttt ggatatcttt tcctaaagag gagatgccaa tatatgcttt
      661 ttctagctaa gcgttttttc tgctatcaag cagagtccgt ttctaattag acgaatgaga
      721 taaatctgcg tgtcaggaaa gttcacctcg tccttgtacg catttcagtt atgcatccta
      781 cttgctctat taacagactt ataatcccta cataatatta actacatcga gagttaaaca
      841 tgtccaaatc tgatttatcc ccagaacaca tattacttag aaatggtgct ctcttaatta
      901 ttcctccact agtcattacc ttcggtcttt ggggcgcatt acctgtagca tacagtccag
      961 atgtattctg gaaagatata cctgaatggt tgggggtatt cgaaaatgtg tttcggattt
     1021 tcgtttttag catcccagga atgctgtact ttggaaaagc agaaaagggg caaccgttag
     1081 gctggtattt atatttcggt ggattattga tctaccttgc aagctatggg gcgcaaatag
     1141 ttcacccggg tagtttatgg agtcaaagca tcattggctt tacagccccg gcttggtcca
     1201 ctgtattttg gtttgctggc atcggtttag tatgtgcccg cagttggctc ccaattccat
     1261 ggtgtcgtgc gatttattta gtaattgcat cctgcttttt gatcttccat atctggcaca
     1321 cggggttggt ctacttcagc ataactcaat aagttgtttt gcaaaacatc taataaatat
     1381 attgaagaac tcttttgaac acaaaa
//
LOCUS       DAOREF010000885         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_90617_length_1405_cov_3.441481, whole genome shotgun sequence.
ACCESSION   DAOREF010000885 DAOREF010000000
VERSION     DAOREF010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90617_length_1405_cov_3.441481"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKI33_15320"
     CDS             complement(<1..293)
                     /locus_tag="PKI33_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C2)-methyltransferase"
                     /protein_id="HNM38439.1"
                     /translation="MLIYDLNLPALESVLKEWNEPAYRAKQIWQGLYQHLYSSTEQFS
                     NLPNSLRSKMAEHFTFNPFTIKTYLDSSDKSTRKTLFQLSDGNLIESVLMRYG"
     gene            498..1403
                     /locus_tag="PKI33_15325"
     CDS             498..1403
                     /locus_tag="PKI33_15325"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNM38440.1"
                     /translation="MLKPMKPKQTFPNNTILWILVGVLVIGGIIAIVSAFSGGGKNAE
                     DEVNAVYTNAAATLAIQQMTLQAASPSVTPTVATPTGTVTFTPQPLATLPTSQGISTS
                     SSSSSGGSGAVGCNNSVFVSDVTVPDDTVMTPGQSFTKTWKLQNTGSCPWTTSFKVSF
                     VSGNAMSGTTTALTTTVQAGQSGDISVAMKAPATTGDAIGYWILTNDSGQNFGSSFYV
                     KIKVGGTSTTGTATTTSTATNTSAPSTPVAVGNVSVTLQCSSPDGGLNFDYSGTTISW
                     TDQSNNESGFYIYINKYNHLYIHIM"
BASE COUNT          396 a          299 c          300 g          410 t
ORIGIN      
        1 tcaccataac gcatcaagac agactcgatt aaatttccat ccgaaagctg aaataatgtt
       61 ttacgagtgg atttatcaga tgagtctaaa taagttttaa ttgtgaatgg attaaaggta
      121 aaatgctcag ccattttgct gcgcagggaa ttcggcagat tgctgaattg ttctgttgaa
      181 gaataaagat gttgatacag tccctgccag atttgcttgg cgcggtatgc aggttcattc
      241 cattctttta agacagattc aagagcggga aggttaagat cgtaaattaa cataaatggt
      301 ttcctatttt acaccagcaa atggaaatcc catttttcgt aggaatggaa cttatctctc
      361 atggagaccg tcataaatct ataaaattct gcccgtatcg tacaagaaaa gtaggaaatt
      421 cgtagggaaa tacctatgtt ttcttaattt tttccaaata taattctaag ccttgcttga
      481 aaacgaagga gaaatttatg ttgaaaccaa tgaaaccaaa gcaaactttt ccaaataaca
      541 ctattctgtg gattctggtg ggagtccttg ttattggcgg cattattgcc attgtgtctg
      601 catttagtgg gggaggaaag aatgcagagg atgaagttaa cgcggtatac actaatgctg
      661 cagcgaccct tgctattcag cagatgacct tgcaggcggc ttccccgtct gtaaccccaa
      721 ctgttgcaac cccaacaggg acggtgacct tcactccaca gccccttgcc accctcccaa
      781 catcgcaggg gatttcgacc agttcttcct cttcgtctgg cggctctggc gcggtgggat
      841 gtaacaactc ggtctttgtc tcagatgtga ccgtgccgga tgatacggta atgacccccg
      901 gacagtcttt caccaagact tggaaattgc aaaacaccgg ctcctgcccg tggacaacgt
      961 cgttcaaagt tagctttgtt tctggcaatg cgatgagcgg gactactact gctcttacta
     1021 ctaccgttca agctggacaa tctggtgata tttcagtagc tatgaaagct cctgcaacta
     1081 caggtgacgc catcggctat tggattttga ccaacgatag cgggcagaat tttggttctt
     1141 ctttctatgt taagatcaaa gtaggtggca cttctacaac aggtactgcc actactacat
     1201 cgactgctac taacacgagc gctccatcta ctcctgttgc agtaggtaat gttagtgtca
     1261 ctctccagtg ttcttcacct gacggcggat tgaattttga ttattcagga acaactattt
     1321 cttggactga tcagtcaaac aatgagagcg gtttttatat atatataaac aaatataatc
     1381 atttatatat acatataatg tgaaa
//
LOCUS       DAOREF010000886         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_90763_length_1404_cov_2.846553, whole genome shotgun sequence.
ACCESSION   DAOREF010000886 DAOREF010000000
VERSION     DAOREF010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90763_length_1404_cov_2.846553"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKI33_15330"
     CDS             <1..180
                     /locus_tag="PKI33_15330"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNM38441.1"
                     /translation="TDIGFINAGQSAPYQIIPDYTIPFLGQTAISTIVAGVVGVIVVA
                     AIIMLVGRGMKAKSS"
     gene            238..1029
                     /gene="cbiQ"
                     /locus_tag="PKI33_15335"
     CDS             238..1029
                     /gene="cbiQ"
                     /locus_tag="PKI33_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNM38442.1"
                     /translation="MHFDAFDRYHEKESFLHRLDPRVKVLVTVAFIISNALLPDAAWV
                     AFAFAWLFLLACNLLSKLGITYTLKRSIIAFPFALIAITILFSMPGKPLATFHLMMSE
                     YTITDMGLLRFLSILVRSWLSVQMAILLVATTRFPDLIHAFEHLRVPAVLTTIIAFLY
                     RYLFVLTDEVFRLLRAREARSAAVTGSKSGGGVVWRAKIAGNMAGQLFLRSYERSDRI
                     YNAMVARGYVGHLYTLNPHEMKSSDYFSTAAAVAIIFITHILGRF"
     gene            1029..>1404
                     /locus_tag="PKI33_15340"
     CDS             1029..>1404
                     /locus_tag="PKI33_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38443.1"
                     /translation="MPVTHITEQFSQPQSSNDVLSVNDLHFSYPDGHVALSGVSLKLY
                     EGDKVALVGPNGAGKSTLMLHLNGILGGKGEVEIAGMRLTRDNLPAIRARVGLVFQNP
                     DDQLFSPTVFEDVAFGPLHMGLP"
BASE COUNT          303 a          302 c          358 g          441 t
ORIGIN      
        1 acagatattg ggtttatcaa tgctgggcaa tctgctccat atcagatcat tcctgattac
       61 accattccat tcctaggaca aactgcaata tctacaattg ttgctggtgt agtgggagtg
      121 attgtggttg ctgcaattat tatgcttgtg gggcgcggta tgaaagcaaa gtcgtcttaa
      181 ggaagtcaag aagcgtggtg cgtaatttgt aaattacgca ccacggaata ggttcttatg
      241 cattttgatg cttttgatcg ttatcacgaa aaagagagtt ttcttcaccg gctcgacccg
      301 cgtgtgaagg ttttggtgac ggtcgctttt attatttcga atgccctttt gccagatgcc
      361 gcctgggtcg cttttgcctt tgcctggctc tttttactgg cttgtaacct gctttcaaag
      421 cttggaatta catacacgct caagcgatcc ataatcgctt ttccgttcgc attgatcgct
      481 attacgatcc ttttttccat gccgggaaag ccgcttgcca catttcattt gatgatgtct
      541 gaatacacga tcaccgatat gggtctgctg cggtttttga gtatcctggt ccgttcatgg
      601 ttatcggtgc agatggcaat ccttttggtg gcaaccacgc gcttccccga tttgatccat
      661 gcttttgaac atttacgcgt tccggcagtg ttgaccacga tcatagcgtt tctctatcgc
      721 tatctgtttg tgttgacgga tgaggttttc cgtctgcttc gggcgaggga agcgcgttcg
      781 gcggcagtga caggctcaaa gtcgggtgga ggcgtggtat ggcgtgcaaa aattgccggg
      841 aatatggcag gtcaattatt cctgcgtagt tacgagcgca gtgaccgcat ctacaatgcc
      901 atggttgctc ggggatatgt gggtcatctc tatacgttga atccgcatga gatgaaatcc
      961 agcgattatt tttcgaccgc agctgctgta gctattattt ttatcactca tattcttgga
     1021 aggttctaat gcctgttact catattaccg aacaattttc acaacctcag tctagcaacg
     1081 atgtgttgag tgtaaacgac ttacattttt cataccctga tgggcatgtt gccttgagtg
     1141 gcgtatccct taagttatat gaaggagata aggtcgcatt ggtgggcccg aatggggcgg
     1201 ggaagtccac gttgatgctg cacttgaatg gaatcttagg tggtaaaggt gaagtggaaa
     1261 ttgcgggtat gcgcctcaca cgtgacaatt tgcctgccat ccgcgcaaga gtgggtctcg
     1321 tcttccagaa cccagatgac caactttttt cgcctactgt gtttgaagat gttgcttttg
     1381 gacctttgca tatggggttg cctg
//
LOCUS       DAOREF010000887         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_91294_length_1400_cov_5.881041, whole genome shotgun sequence.
ACCESSION   DAOREF010000887 DAOREF010000000
VERSION     DAOREF010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91294_length_1400_cov_5.881041"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..825)
                     /locus_tag="PKI33_15345"
     CDS             complement(25..825)
                     /locus_tag="PKI33_15345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNM38444.1"
                     /translation="MIQPPASYSDPSLGQQPEPIPTRPPTYVRMPTLQPIVTYALIGV
                     TIFVYLLQVLSSVLFGDSSYGIDWVTLYGARISDAIYAGQLWRLFTPMFLHGSIPHIF
                     FNMYALFSVGSLLERHFGHGRFLLLYVLGGFAGNVLSFLFTQGYSVGASTAVFGLIAA
                     EAIFFYQNQKLFGSHAKQAISNAIFIIAINLFLGLAPSIDTWGHIGGLLGGAMFAWFA
                     GPKWTVVGMPPEYHLDDERESREIITGALLVALIFGGLTVWAFIQHAV"
     gene            complement(822..>1400)
                     /locus_tag="PKI33_15350"
     CDS             complement(822..>1400)
                     /locus_tag="PKI33_15350"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNM38445.1"
                     /translation="LIVISGPSGVGKDSVVQRMKDRGFPFHFVVTATTREKRANETHG
                     VDYFFLSKDEFAHMIEEDELIEYAIVYGDYKGIPKEQVRNAFASGKDVVMRIDVQGAE
                     SVRKLAPEALLIFITCESEEELERRLRERKTESPDSLSLRIATARKELQRLEAFDYVI
                     VNHDFHLDDTVDKVRAIIDAEHLKVHHRMVTL"
BASE COUNT          386 a          335 c          371 g          308 t
ORIGIN      
        1 caagacgggc ttttttatta tttgttacac agcatgttga atgaatgccc aaacggttag
       61 cccgccgaag atgagcgcaa caagcagtgc gccagtgatg atctctcgac tttctcgttc
      121 gtcatccaga tgatattcag gcggcatacc gaccaccgtc cacttgggac ctgcaaacca
      181 ggcgaacatt gccccgccga gtagaccgcc aatgtgcccc cacgtatcaa tgctgggagc
      241 caagccgagg aagagattga tggcgataat gaagatggca ttgctaatgg cttgcttggc
      301 atgtgaaccg aataattttt ggttttggta aaagaagatg gcttctgcgg caatgagacc
      361 aaataccgct gtggaagcac cgacggaata gccttgggta aagagaaaag acaatacatt
      421 cccggcaaaa ccaccgagaa catacagcaa caagaagcgc ccatgcccga agtgacgttc
      481 tagcaaactc cccaccgaga aaagcgcata catattaaag aaaatatgcg ggatggagcc
      541 atgcaggaac atgggcgtga acaaacgcca aagttgcccg gcatagattg cgtcactgat
      601 gcgagcgccg tataaagtca cccaatcaat gccgtagcta gagtcaccaa aaaggacaga
      661 actgagaact tgtagaagat aaacgaaaat ggtcacacca atgagtgcat aggtgactat
      721 gggttgcaag gtgggcatcc gcacgtaagt gggcggacgg gtaggaatcg gttcgggttg
      781 ttgacccagt gacgggtccg aataagaggc gggaggttga atcacaaggt caccatgcgg
      841 tggtgcactt tcaaatgttc ggcatcaatg atcgcgcgga ctttatccac agtgtcatcc
      901 aaatgaaaat catgattgac gatcacatag tcgaaggctt cgagccgttg cagttctttg
      961 cgggcggtgg caatgcgtag agaaagggag tctggcgatt cggtcttgcg ttcgcgcaaa
     1021 cgtctttcta attcttcttc gctttcgcac gtgatgaaaa tgagcaaggc ttcaggcgca
     1081 agcttgcgaa ccgactcagc tccttgcacg tcgatgcgca tgaccacatc tttgccgctg
     1141 gcgaaggcat tgcgcacctg ttctttggga atgcctttgt aatcgccata gacgatggca
     1201 tattcgatca actcatcttc ttcgatcatg tgcgcaaatt catcttttga gaggaagaaa
     1261 taatccacgc catgggtctc attggctcgc ttctcgcgag tggtggcagt caccacaaag
     1321 tggaagggaa acccgcggtc tttcatgcgt tgcaccacag agtctttgcc tacaccagaa
     1381 ggaccagaga tgacgatcaa
//
LOCUS       DAOREF010000888         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_91706_length_1397_cov_3.034277, whole genome shotgun sequence.
ACCESSION   DAOREF010000888 DAOREF010000000
VERSION     DAOREF010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91706_length_1397_cov_3.034277"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKI33_15355"
     CDS             complement(<1..625)
                     /locus_tag="PKI33_15355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM38446.1"
                     /translation="MADWIGQTIGKVRVEKYLARGGMAEVYLGTHLTLDRPVAVKVMH
                     SFVESEPELIARFQREAKAVAGLRHPNIVQVYDFDTHDGHPYLVMEYLKGPSLASLLR
                     ELHKNGARLSLSQVAQLLKSLAAGIDYAHSQGIVHRDIKPANIILNNKSGNYAVDQLP
                     RDTECIITDFGLVRLSNSTTQTASGVVSGTPAYMSPEQAQGSAIDSRS"
     gene            778..>1397
                     /locus_tag="PKI33_15360"
     CDS             778..>1397
                     /locus_tag="PKI33_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38447.1"
                     /translation="MQNFTGNIGRTVTNRIGGAVAAIGAAIFMALCGALMAFVLAPQQ
                     AIKAANIEKMPQMDAAYVSAAAPGTDILITGYLNGSAPNPQLPKFIAYEAEQWDVSEN
                     KNTDGTPQAPSGSWEHVETIVPDLTLDMSGTPVNILSANNATLSGSNLHEQLVTKSSA
                     FTADYKGQKLGEGSLRYTGFFSGDLVTVLGKKSSGDGVVPEELFAG"
BASE COUNT          350 a          300 c          390 g          357 t
ORIGIN      
        1 cagatcggct gtctattgca gagccttgtg cctgctctgg tgacatatag gctggggtgc
       61 cgctgacaac accggaagcg gtttgagtag tggagtttga gaggcgcacc aaaccaaagt
      121 ctgtgatgat gcattcggta tcgcgtggaa gttgatcgac ggcatagttg ccgcttttat
      181 tattgaggat gatattggcg ggtttgatat cgcgatgaac gatgccttgt gagtgagcat
      241 agtcgatgcc tgccgcaaga gacttgagca attgcgccac ttgggaaagg gacaaccttg
      301 cgccattctt atgcagttca cgcaagagac tcgcaagcga tggacctttg aggtattcca
      361 tcacaagata gggatgccca tcgtgggtgt caaaatcgta gacttgaaca atattgggat
      421 ggcgtagacc ggcaacggct ttggcttcgc gttggaagcg cgcgatcaat tctggttcac
      481 tttccacgaa gctatgcatc actttgaccg ctacggggcg atcgagagtg aggtgtgtgc
      541 ccaagtagac ttctgccatt cccccgcgtg ccagatattt ttctacacgg actttgccaa
      601 tcgtttgtcc gatccagtct gccatgaatg aattatacag tggaaattag agcgattgtg
      661 tctgaaaact tcttcgaaca tggtattgaa atggagcctg attagtactt ccagtggatg
      721 atgccgatgc ggaataaatt ttataatgcc gcatcaaacc aaatgaggag aatgagtatg
      781 caaaatttta ccggcaatat aggtaggaca gttaccaatc gaattggtgg ggcggtggct
      841 gccattggtg ctgcgatctt tatggcgctg tgtggcgcat tgatggcatt tgtgctggca
      901 ccgcaacagg cgatcaaagc cgcaaatatt gagaagatgc cgcaaatgga tgccgcctat
      961 gtgagtgctg ctgcccccgg cacggatatt ttgatcacgg gttatttgaa tgggtctgca
     1021 ccgaacccgc aattgccgaa gtttattgct tatgaagcag aacaatggga tgtgagtgag
     1081 aacaagaata ccgatggcac accccaagcg ccttctggtt cttgggaaca tgtggagaca
     1141 atcgttccag acttaaccct ggatatgagc ggaacgccgg ttaacatcct ttcggcaaat
     1201 aatgcgacgt taagcggctc aaaccttcat gagcagctgg tgacaaaatc cagcgctttc
     1261 actgccgatt acaaaggaca gaagttagga gaaggttccc ttcgttatac aggctttttc
     1321 agcggtgatt tggtgaccgt gctcggcaag aaatcttctg gagatggagt ggtgccagaa
     1381 gaacttttcg ctggtga
//
LOCUS       DAOREF010000889         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_91950_length_1395_cov_3.850000, whole genome shotgun sequence.
ACCESSION   DAOREF010000889 DAOREF010000000
VERSION     DAOREF010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91950_length_1395_cov_3.850000"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="PKI33_15365"
     CDS             complement(<1..1278)
                     /locus_tag="PKI33_15365"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNM38448.1"
                     /translation="MKRLNFIVIIALVASTLGACQVLPRINDTRPNFLIIVTDDQRYD
                     TMEYMPNTQELIFDQGVTFSKGYITTPFCCPSRASILTGLYAHNHWVQVNEDPLEFRT
                     MVEDIHRNGYYTGLVGKYLNSWKGDMRPEYDFWVSFWGGTMKNYYNPDLNVNGTWSKH
                     TGYSTYLFRDFGVQFIEEASKQSKPFLLMYTPNAPHAPFTPAEEDVSLLTDLAPFRPL
                     SFNEEDVSDKPDSISNMAPLSEEDIKSIDNTRRRQLLTLMALDRTIPDLINKLKETGE
                     LDNTVIIFISDNGKHWGEHRLDSKSTAYEESVKVPFAIRYPPLIPTPYVDNRIVANID
                     IAPTIYELSETRLPDLMDGTSLVSLFSGETEWRDHILLEAWPDRGAWSAIHTGDYVYI
                     ETQGDLSEFYDLTLDPFEMDNAINDPQYAEMIAQ"
BASE COUNT          384 a          320 c          310 g          381 t
ORIGIN      
        1 ttgggcaatc atctctgcat actgtgggtc attgatggca ttgtccattt cgaagggatc
       61 aagcgttaaa tcataaaact cagacagatc gccctgtgtt tcaatataaa cgtaatcacc
      121 cgtatgaatg gcagaccaag caccacggtc tggccaggct tcgagcagga tgtggtctcg
      181 ccattcggtt tcccccgaaa aaagggaaac taaagaagtc ccgtccatta agtccggcaa
      241 acgcgtttca gatagttcat aaatcgtcgg tgcaatatca atattagcaa cgatgcgatt
      301 atccacatat ggagttggaa tcagaggtgg atagcgtatc gcaaatggca ccttaaccga
      361 ttcttcatac gctgtgcttt ttgagtccaa gcgatgctcg ccccaatgct tgccattatc
      421 cgagataaaa ataatgacag tgttatctaa ctcgcccgtt tctttcaatt tattgatgag
      481 gtcagggatg gtgcgatcaa gcgccatgag agtcagcaac tgacgtctac gcgtattatc
      541 aatagactta atatcctctt cggataaggg agccatattg gagatcgaat ctggcttatc
      601 cgaaacatct tcttcgttga aactcaatgg gcggaaagga gccaagtcag ttagtaaact
      661 gacatcttct tccgcaggcg taaaaggagc atgcggcgca tttggcgtat acatcagaag
      721 aaaaggcttc gactgcttac tagcttcttc aataaactga acgccaaagt ctctaaacaa
      781 ataggttgaa tacccggtat gtttggacca tgtcccattc acattcaaat cgggattgta
      841 atagttcttc atggttccac cccaaaatga gacccaaaaa tcatattcgg ggcgcatatc
      901 ccctttccat gaattcaggt atttgccaac caagccagtg taatagccgt tgcggtgaat
      961 atcttccacc atcgtccgga attcgagtgg gtcttcattg acctgcaccc aatgattatg
     1021 agcatacaac ccggttagaa tactggcacg gctcggacaa caaaacggag tggtgatgta
     1081 tcctttggaa aaggtcacgc cctggtcaaa tattaattct tgagtgttag gcatgtattc
     1141 cattgtgtca taacgctggt catctgtcac aatgatgagg aaattcgggc gtgtatcatt
     1201 gatacgcgga aggacctggc aagctcctaa cgtggaagct accagtgcaa taatgactat
     1261 aaaattgagt cttttcatta cagtaccttg taagagaccg ttacgcccga cgggttgttg
     1321 ttcgtttcgg gcgtggcttt tgctccatct tcggaacctt atcccctttt aattcccgaa
     1381 tctcatcctc agtta
//
LOCUS       DAOREF010000890         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_92076_length_1394_cov_4.733383, whole genome shotgun sequence.
ACCESSION   DAOREF010000890 DAOREF010000000
VERSION     DAOREF010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92076_length_1394_cov_4.733383"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKI33_15370"
     CDS             complement(<1..486)
                     /locus_tag="PKI33_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM38449.1"
                     /translation="MRTTFTKLENFGHSLEAPAYVLKPRNSEEIYSAFKQAKKMGLTV
                     TARGAGRSYNDASLNGGGIVLNMTGMDQILEWDPATGLVRCEPGVTLEKLWQQVEPDG
                     WWPPVVSGTMRTTLGGCLSANIHGKNNFKMGTIGEHVVEFTATLPTGAEVTCSPKKNA
                     DL"
     gene            complement(495..1277)
                     /locus_tag="PKI33_15375"
     CDS             complement(495..1277)
                     /locus_tag="PKI33_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNM38450.1"
                     /translation="MSDLKPATPLNPRRRAIVVGASVGMGAALSKKLAKEGYVVALVA
                     RQKEKLNELCAEINESTGEVRAFAYPHDVSNYKEAPELLKNIVAEIGGLDTFVFMAGV
                     NYPPGGNDIYNFENDRQMVEINLIGAMAWLSPVAEMFQAAKYGQIVGVGSVAGDRGRV
                     GNPGYNTSKAGLATYLEALRNRLTRHGVNVLTVKPGFVKTEMLKAAVGPTPFAIPVEK
                     AVDDIYKAMQKRKQSIYTPFFWQYIMLIIRHIPSFIFRRLSF"
BASE COUNT          342 a          325 c          345 g          382 t
ORIGIN      
        1 caagtccgca ttctttttgg gcgaacaagt cacttccgcg ccggtgggta gtgtggctgt
       61 aaactccacc acatgttcac cgatggttcc catcttaaaa ttatttttgc cgtggatgtt
      121 tgcactgaga caaccgccca atgttgtgcg catcgtacca gagacgacgg gcggccacca
      181 gccatctggt tcgacttgtt gccaaagttt ttcaagcgtg acgccgggct cgcatcgtac
      241 caagccggta gctggatccc attccagaat ttgatccatg ccggtcatgt tgagcacaat
      301 tccgccaccg ttgagggaag catcgttata acttcgcccg gctccacgag ctgtgacggt
      361 aagccccatt ttctttgctt gcttgaatgc agaatagatt tcttcgctgt tgcgaggttt
      421 tagaacatac gccggggctt caagagaatg accaaaattt tcgagcttcg taaaagtagt
      481 acgcatgagg ttcgttagaa agagagacgt ctgaatatga aagaggggat atgccgaatg
      541 ataagcatga tgtattgcca gaagaaagga gtatagatgg attgctttcg tttctgcatg
      601 gctttgtaaa tgtcatctac cgctttttcg accgggattg cgaacggagt gggacctact
      661 gcggctttga gcatttcggt tttcacgaag cctggtttga cggtgagcac attcacccca
      721 tgacgggtga ggcggtttcg taaggcttcc agataagttg ccaacccagc tttggatgtg
      781 ttgtagccgg ggttgcctac tcgtccacgg tcaccagcaa cggagccgac accaacaatt
      841 tgcccatatt ttgcggcttg gaacatttcc gcgactgggc tgagccatgc catagcccca
      901 atcaggttga tttccaccat ttggcggtcg ttctcgaaat tgtagatgtc gtttccaccg
      961 ggcgggtaat tgacgccagc catgaaaaca aaagtatcta aaccgccaat ttcagcgact
     1021 atattcttta agagttctgg agcttcttta tagtttgaaa catcgtgcgg gtaagcgaag
     1081 gcacgtactt ccccagtaga ttcgttgatt tcggcgcaga gttcattcag tttttctttt
     1141 tgacgggcaa ctaaagcaac gacatacccc tctttggcaa gttttttaga gagcgcagct
     1201 cccattccaa cagaagcgcc gaccacaatg gctctgcggc gcggatttaa tggtgttgca
     1261 ggttttaagt cactcacaat gacctcgtat taagtattta ttcatgtcat tctaacacag
     1321 caataaatga cctttcgggt cgaaatagaa tatgtatatg aaagtgtata atagtacaaa
     1381 ggatgtctcg acag
//
LOCUS       DAOREF010000891         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_92095_length_1394_cov_2.691561, whole genome shotgun sequence.
ACCESSION   DAOREF010000891 DAOREF010000000
VERSION     DAOREF010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92095_length_1394_cov_2.691561"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKI33_15380"
     CDS             complement(<1..901)
                     /locus_tag="PKI33_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38451.1"
                     /translation="MNKQELFKQADYTFQRGNRELAKKYIADILAVYPDDEQTWMLLA
                     RVVEEKERKVQCFERVLKINPKNEEAKLALVRVRSTINPTLPLPKQVKPLAPKPNPYR
                     NLIRGALVAVVAFLLFGTTTYVIALNNPNSQVARVLAIATPTVMSEVSLAADIAPETR
                     AEVNKIYPEYAPLVDALLGMALSTTKDGMEGAPERPGDAIVASDTSGIEVKTMVEKSL
                     PQPGTMTSITITEQQLTSWVAMEMKNSPDLPLSDVQIYLRDGKVQIWGMVTGSDSSTS
                     ALVVGELSIDANKLPYFKIDSMQI"
     gene            complement(971..>1394)
                     /locus_tag="PKI33_15385"
     CDS             complement(971..>1394)
                     /locus_tag="PKI33_15385"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-hexose 4-ketoreductase"
                     /protein_id="HNM38452.1"
                     /translation="EEKLSYEDMRKKLSESLKRAFRPEFINRVDAIVIFRSLNKEDIR
                     KIVSLELDKVAERLKDHDLNLVAIEEALALLADQGYDSEFGARPLRRVIQMKVEDPLS
                     DKLLSGEFVDGDTITVKIDDDGEVSLEKSSDPVAEPSL"
BASE COUNT          338 a          356 c          326 g          374 t
ORIGIN      
        1 cgatctgcat cgaatcgatc ttgaaatacg gcaatttatt ggcatcgatc gaaagctcac
       61 caaccaccaa agccgaagtt gacgagtcac tgccagtcac cataccccaa atttgcacct
      121 tgccatctcg cagataaatc tgcacatcgc tcaggggcag gtctgggctg tttttcattt
      181 ccattgccac ccacgacgtt aattgttgct cggtgatagt aatggaagtc atcgtgcctg
      241 gctgcggcaa acttttttcc accatagttt tcacttcgat accagatgta tcagaagcaa
      301 caatggcatc gccggggcgt tcaggagcac cttccatacc gtctttggtg gtgctcaatg
      361 ccatacccaa taacgcatcc acgagcggag catattctgg gtagatttta ttgacttccg
      421 cacgggtctc tggagcaata tccgcagcga gagatacttc actcatcaca gtaggagtgg
      481 caatcgccaa tactcttgca acttgtgaat tcgggttatt caatgcgatg acgtaggtgg
      541 tcgtaccaaa caataaaaag gcaaccacag ccaccaacgc gccccggatt aagttgcgat
      601 acgggttcgg ctttggcgcc aacggtttaa cctgtttcgg gagcggcaat gttgggttga
      661 tcgtagagcg cacacgtaca agtgctaatt tggcttcttc atttttgggg ttaattttga
      721 gtacacgttc aaagcattgt accttgcgtt ctttttcctc cactacacga gcaaggagca
      781 tccatgtctg ctcgtcatcg gggtaaacgg caaggatatc tgcaatatat ttttttgcca
      841 gttcgcggtt tccgcgctgg aaggtgtaat cggcttgctt aaaaagttct tgcttattca
      901 tctttaaatc gcaggggcgc ggtcatgcca cattgtgaca tcagccgcgc ccctacatca
      961 tattctttct ctacaggctc ggctcagcaa cagggtcaga tgatttttcg agtgagacct
     1021 cgccatcatc gtctatcttg acagtaatgg tatcaccatc tacaaattcg ccggagagca
     1081 atttatcaga aagcgggtct tccaccttca tttgaatgac acggcgcagc ggacgtgcac
     1141 cgaactctga gtcgtagccc tgatctgcca gcaaagccaa agcttcttca atggcaacca
     1201 ggttcaggtc atggtctttg aggcgttcgg caaccttatc caattcaagg ctgacaatct
     1261 tacgaatatc ctctttattg agcgagcgga agatgacgat cgcatccaca cggttgatga
     1321 actcagggcg gaaggcacgc ttgagcgact cgctcaactt cttgcgcatg tcttcgtaag
     1381 agagtttttc ttcg
//
LOCUS       DAOREF010000892         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_93134_length_1386_cov_6.525169, whole genome shotgun sequence.
ACCESSION   DAOREF010000892 DAOREF010000000
VERSION     DAOREF010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93134_length_1386_cov_6.525169"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PKI33_15390"
     CDS             <1..1116
                     /locus_tag="PKI33_15390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM38453.1"
                     /translation="TGGISILFFPRFQAFVDQKFLGIKLPYQHLPEIYSSRITTSTSL
                     PSLLGLLETDVFPSLLIRQYAFLQVSEKGLIKLMDKGIPNLATFDIETLTQKAGEYIP
                     SLSQQDDGPRLILPLKVGTQTLGFWLLGKRDPDDLYPQVEIPILQSLANQTAIALSNI
                     LQTDQLRNIYESNIDRVEKERKRVARDLHDSVLNQLANMRNSLDQRTLPAEFLSTYDG
                     LKQRIREIISDLRPPILDQGLAYAISELVEDMREEASHMDINLDLQTSGERIPEKNET
                     HLFFIVREACMNVLKHANAKTLNISGNITPTRVELSIQDDGKGLNIDLQKEVNTLLEE
                     EHFGLVGMFERARLINADLNINSNENSGTTIHITWQS"
BASE COUNT          411 a          366 c          272 g          337 t
ORIGIN      
        1 acaggtggaa tctctatcct gttctttccg cgctttcaag cctttgtgga tcaaaaattt
       61 ctgggcatta aactgcccta tcaacacctt cctgaaatct attccagccg tatcacaacc
      121 agcacctccc ttccgagcct gcttggttta ctagagacgg atgtctttcc aagtctattg
      181 atccgtcaat atgctttttt gcaagtcagc gaaaaagggc tgataaaact tatggacaaa
      241 ggcatcccaa atctcgccac gtttgatatt gaaaccctga cccaaaaagc aggagaatac
      301 attcccagcc tttctcagca agatgacgga cctcgcctca tccttccgct caaagtgggt
      361 acccaaaccc ttggcttttg gctactcggt aagcgtgacc ctgacgactt gtatccacag
      421 gttgagattc ccatcttaca gtcgttggca aaccaaaccg ccatcgccct cagtaacatc
      481 cttcaaaccg atcaacttcg gaacatctac gaatccaata ttgaccgagt tgaaaaggag
      541 cgaaaacgtg ttgcccgcga cctgcatgac agtgttctta atcaactagc caacatgcgc
      601 aacagcctcg accaaagaac ccttcccgct gaatttctat ccacctatga tggtctcaag
      661 caacgcatcc gtgaaatcat cagcgacctt cgtccgccca tactggatca aggacttgcc
      721 tatgcaatca gcgagttagt agaagacatg cgagaagaag caagccacat ggacattaat
      781 cttgacctcc aaaccagtgg cgaaagaatc cctgagaaaa acgaaaccca ccttttcttt
      841 attgtgcgtg aagcctgtat gaatgttttg aaacatgcca atgcaaagac attgaatatc
      901 tcaggaaata taacccccac ccgagtggag ctatccattc aagatgatgg taaaggctta
      961 aacattgact tgcaaaaaga agtcaacaca ttactcgaag aagaacactt cgggcttgtt
     1021 ggcatgttcg aacgtgctcg gttgattaat gcagatctga acataaactc caacgaaaat
     1081 tctggcacaa ccatacatat tacctggcag tcttaataaa actgtctttt cacttttaaa
     1141 acattacacg ccgggcctta caccggcgtg caccccaggt cccctaatcg caatcggata
     1201 tcccctttcc tgttccaact gcgctggaaa gcaaggttat tcagacatca aacgaataaa
     1261 ttcagaagtt agggcagggt caaagtgatg ccccgtctca ttacggataa attgaagagc
     1321 atcgctgcga gaccatgctt tacgatatgg gcggtcagac gtaagtgcat cgtaaacatc
     1381 ggcaaa
//
LOCUS       DAOREF010000893         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_93311_length_1385_cov_2.657143, whole genome shotgun sequence.
ACCESSION   DAOREF010000893 DAOREF010000000
VERSION     DAOREF010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93311_length_1385_cov_2.657143"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1385
                     /locus_tag="PKI33_15395"
     CDS             <1..>1385
                     /locus_tag="PKI33_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNM38454.1"
                     /translation="EHLLIGGFANSEKVVMTIRTGELPPLSVETLPMPVPRYYWRSQV
                     YDQYLGSGWGSSVILKQKVSANTPLLPAFLNNYRPIHLDVQMTEPEGKLFWSGILFRT
                     DVPFTAQWRLRPTSDLFANQTVLMQSDIFIATSKATTYRAEVYLPTPTISQLHSATTD
                     YPEEIRERYLELPSELPKRVRDLALDITAGVSNPYEKAKVIESYLRSNYPYDLNVPAP
                     PTDQDATDYFLFELKRGYCDYYATAMVVLARANGLPARFVSGYSPGSYDASNAQYIIR
                     ELNAHSWAEVYFPGIGWVEFEPTASLPEIERSESSTTQSEEALLQDIAPQEPKTKPLA
                     VSWIEKKLIWVSPFAGILAIALIYFMFIERWFYLRLEPVTGIHRIYQNFYRIGKIFTG
                     QISQAETSSEFLTNIIRTLEDIETIKNNTKRLNQTKKNAEALTSIYNRSLFTNYRAKK
                     ADLVLAWNLWAS"
BASE COUNT          382 a          355 c          272 g          376 t
ORIGIN      
        1 gaacatctgc tcattggcgg ctttgccaac tctgaaaagg tcgtcatgac gattcgaact
       61 ggagaacttc cgccgttgag tgtcgaaacg ttgcccatgc ccgttccgcg ttattactgg
      121 cgcagccagg tctacgacca atatcttggc agcggttggg gctccagtgt cattctcaaa
      181 caaaaagttt ctgccaatac gcccttatta cccgcctttt tgaataacta tcgccctatt
      241 catttggatg tgcaaatgac cgagcccgaa ggaaaattat tctggagtgg gattctattt
      301 cgtacagatg taccttttac ggctcaatgg cggctccgcc ctacatctga cctgttcgca
      361 aaccagacgg ttttaatgca gtccgacata tttattgcta ccagcaaggc aacaacgtat
      421 cgcgctgaag tctaccttcc gacgccaacc atttcacagc tgcattccgc gacaacagat
      481 taccctgaag aaattcgtga acgttatctt gaactcccaa gcgagttacc aaaacgggtc
      541 cgcgaccttg ctttggatat cacagcaggc gtgagcaatc cttatgaaaa agccaaagtc
      601 attgaaagct acctgagatc caattatcca tacgacctca atgtaccagc cccacccaca
      661 gatcaagatg ccacagatta tttccttttt gaactcaaac ggggctactg tgactattac
      721 gccaccgcta tggtcgtcct tgcccgcgca aacggtttac cagcccgttt tgtttctggg
      781 tattccccag gttcttatga tgcgtcaaat gctcaataca taatccgtga gttaaatgct
      841 cattcgtggg ctgaagtcta ttttcccggc attggctggg tcgaatttga accaaccgct
      901 tccctccccg agattgaacg ctctgaatca tccacaactc aatcggaaga agctctatta
      961 caagatatcg ctccgcaaga gcctaaaaca aagcctctgg cagtttcttg gatagaaaaa
     1021 aagctaattt gggtttcacc attcgcgggg atccttgcaa tagcacttat ctatttcatg
     1081 ttcattgaaa gatggttcta tctacgcctt gaacctgtca ctggtattca tcgtatctat
     1141 caaaactttt atcgcatcgg caagattttt accggtcaaa tctctcaagc ggagacctcc
     1201 agtgaattcc ttaccaatat cattcgcaca ctggaagata tcgagactat caagaataat
     1261 acaaaaagac tcaatcaaac aaaaaagaat gcagaagcat taacgtctat atacaaccgc
     1321 tctttgttta caaactatcg tgctaaaaaa gcagatttgg tcttagcttg gaatttatgg
     1381 gcttc
//
LOCUS       DAOREF010000894         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_93936_length_1380_cov_4.100377, whole genome shotgun sequence.
ACCESSION   DAOREF010000894 DAOREF010000000
VERSION     DAOREF010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93936_length_1380_cov_4.100377"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1380
                     /locus_tag="PKI33_15400"
     CDS             <1..>1380
                     /locus_tag="PKI33_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38455.1"
                     /translation="MFYLNGVYKPFEPYGPLTNKAPFAFLIPGFAEYLFGAGLRTGRY
                     FSIFLSLLTLLGVWVVSRRWAGNWLAAFSVYFFVLSPMIIKLYALAVSEVIIACILAW
                     VCVLVLAEERPLWQIILASFLAAAAVFARQNVVPILPFMILYIFWQHGKSKGIWSFGT
                     TSIFFLLFHIIYWPRVMTIWAPWLPESLTPFLNSFRVPTDSVPIWDPTIDFWGRLNSF
                     FQGIRYHFIPLVGGFFGLLLIPPRAEWKSASAIRAAVFLAVSYFVLAAMHGWAAIASQ
                     YESYSCVFCFSNYLGFFDPLGILFFIIIFSYAWNRNPNSVVQVVIVFSALVIATGIGY
                     SLFEKVGNGLLNTPVIPRLRSSGLGFVTIFDFLTHGFDMPLVQIKRWISASLGFIIGI
                     AVIFIAFFIWKKRNAPRFAHVLANTFLISGLILSPILHLGGSQIDCQTDIIRDHEELG
                     TYLASIIPA"
BASE COUNT          274 a          297 c          309 g          500 t
ORIGIN      
        1 gcatgttcta tttgaatgga gtttacaaac cattcgagcc gtatggtccg ctgaccaata
       61 aagcgccttt tgcatttcta attccaggtt ttgctgaata ccttttcggc gcagggttac
      121 ggacggggcg ttatttctca atctttttaa gtctattaac ccttttaggg gtctgggttg
      181 tatcacgccg ttgggcgggt aattggcttg cggcattttc tgtctatttc tttgtgctta
      241 gcccaatgat cattaagctc tatgctcttg ccgtctcaga agtgatcatt gcctgtattc
      301 tggcttgggt ttgtgtcttg gtattggcag aagaacgccc actttggcag attattcttg
      361 catcattttt ggcggctgcg gcagtatttg cccgtcaaaa tgtggtgccg atattaccct
      421 ttatgatctt atacatcttt tggcaacatg gaaagtcaaa aggtatatgg tcatttggta
      481 caacatccat attctttcta ttgtttcata ttatttactg gccgcgtgta atgaccattt
      541 gggcgccgtg gttgcctgaa agtctcacac cttttctgaa ttcttttcgt gtgccaacag
      601 actccgtccc gatctgggat cctactatcg atttttgggg acgacttaat tctttcttcc
      661 agggaatccg ataccatttc attccgttgg ttggcggttt cttcggactt ttattaattc
      721 cacctcgcgc cgagtggaag tctgcttctg caattcgagc ggctgttttt ctcgctgtga
      781 gttattttgt gttggctgct atgcacggtt gggcggcaat agcaagccaa tatgaaagct
      841 attcctgtgt gttttgtttt tccaactatc tcggcttctt tgacccttta gggattttat
      901 ttttcatcat aatcttttca tatgcttgga atcggaatcc taattctgtt gtccaggtcg
      961 taattgtttt tagcgcattg gtaatcgcta cgggtatagg ctattctttg tttgagaaag
     1021 tcggtaatgg gctcttaaac accccagtga ttccgcgcct tcgttctagt ggacttggct
     1081 ttgtaacgat ttttgacttt ttgacgcatg gctttgatat gccgcttgta cagatcaagc
     1141 gctggattag tgcttctctt ggctttatta taggaatagc tgttattttt attgctttct
     1201 tcatttggaa gaagcgaaat gctccacgat ttgcccatgt gcttgcgaat acattcttaa
     1261 tcagcgggct tatcctttct cccattctcc accttggtgg aagccaaata gactgtcaga
     1321 ccgacatcat ccgtgaccat gaggagttgg gaacttacct tgcttcgatc attccggctg
//
LOCUS       DAOREF010000895         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_93944_length_1380_cov_3.287547, whole genome shotgun sequence.
ACCESSION   DAOREF010000895 DAOREF010000000
VERSION     DAOREF010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93944_length_1380_cov_3.287547"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKI33_15405"
     CDS             <1..914
                     /locus_tag="PKI33_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNM38456.1"
                     /translation="DFQYYDVWAPLSKKKVPLTFEQAVDHISDSLAPLGKEYVDTLRQ
                     GCLKDRWVDSTANTGKMNGAFSYGTPETHPFIMMSFTNDIGSMSTLAHELGHSMHSYL
                     SWKNQPVTYSNYSLFVAEVASNFNQAMMRGHLLKTIKDKNFLISLIEEAVSGNFFRYF
                     FQMPTLARFELETHQRAERGEPLTADSMQNLMADLFTEGFGPKVKIDRPRVGMVWSTF
                     SHLFADYYVYQYATGISGAHALSARILRGEPNAAEDYLGFLKAGSSKYPLDVLKGAGV
                     DLTTPQAVEETFAVMEGYIDRLEELVG"
     gene            1066..>1380
                     /locus_tag="PKI33_15410"
     CDS             1066..>1380
                     /locus_tag="PKI33_15410"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNM38457.1"
                     /translation="MAQTQIACPRCRQLITANVEQLFDVTADPQAKQRLLSGQVNHAR
                     CPHCGYQGRLATPVVYHDNEKELLLTFFPAELGLPVNEQERMIGPLIKQVMDRLPAEK
                     RKG"
BASE COUNT          352 a          385 c          314 g          329 t
ORIGIN      
        1 aggacttcca gtattacgat gtatgggctc cgctctcgaa gaagaaagtt ccgctcacct
       61 ttgaacaagc cgtggaccac atcagtgata gccttgcccc gcttggcaaa gagtatgtag
      121 acacacttcg ccaaggatgt ctcaaagacc gctgggtcga ctcaactgcc aacactggca
      181 aaatgaacgg cgctttttcc tacggtacac cagaaacaca tccctttatt atgatgtctt
      241 tcaccaacga catcggcagc atgagcacct tggcgcatga attgggtcac tccatgcact
      301 catatctctc atggaaaaac cagcccgtca cctactcaaa ctattcgctc tttgtcgccg
      361 aagtagcttc caacttcaac caagccatga tgcgcggaca tttgctcaag accatcaagg
      421 ataagaattt cttgatctca ctgatcgaag aagccgtgag cgggaacttc ttccgctact
      481 tcttccaaat gcccaccctc gcccgcttcg aactggaaac gcatcagcgc gccgaacgtg
      541 gcgaacccct caccgcagac tccatgcaaa acctaatggc agatttgttc accgagggct
      601 ttggtcctaa agtgaagatc gaccgcccgc gtgtgggcat ggtttggtct accttctcgc
      661 atctcttcgc ggactattat gtctatcagt acgccacagg catttctggt gcgcatgcct
      721 tatctgccag aatcttgcgc ggcgaaccga atgcagcaga agattacctc ggcttcctca
      781 aggcgggttc ttccaaatac cccttggacg ttctcaaggg tgccggtgta gacttgacca
      841 cgccgcaagc cgtggaagag acctttgccg taatggaagg ctatatcgac cgcttagagg
      901 aattagtcgg gtagtctgat agcgcattcg tctcatcgtt aaatcattac gcatcacggg
      961 ttacttacat gtaaaacgtg gtgcgtaatt tatgtctctc aagattaatt cgtgtaaaat
     1021 gggttctgta ttacccaatc ccaaatcgtc aaatcggaaa tcgtaatggc tcaaacacaa
     1081 attgcatgcc cccgttgtag acaattaatt actgccaatg tagagcagtt gttcgatgtg
     1141 accgcagacc cgcaagccaa acagcgcttg ttaagcggac aagttaacca cgcccgttgc
     1201 ccgcactgtg gctaccaagg gcgcctcgct accccggttg tgtatcacga caatgaaaaa
     1261 gaactcttgt tgaccttctt ccctgctgag ttagggttgc ccgtcaacga acaagagcgg
     1321 atgatcggtc cactcatcaa gcaggtgatg gaccgcctcc ccgctgaaaa acgcaaaggt
//
LOCUS       DAOREF010000896         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_94187_length_1378_cov_4.868481, whole genome shotgun sequence.
ACCESSION   DAOREF010000896 DAOREF010000000
VERSION     DAOREF010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94187_length_1378_cov_4.868481"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1378
                     /locus_tag="PKI33_15415"
     CDS             <1..>1378
                     /locus_tag="PKI33_15415"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNM38458.1"
                     /translation="ALMAWLAWMGGSIGVPYTRLSIGVAFTLIVVAGIGLWMKRKEEF
                     KADWDRHRQFFVLVEIVFLTFFLIDLLIRIGNPDMWHPSKGGERPMDFSYFNAVLKST
                     SFPPYDPWYAGGYINYYYYGFVLAGTPVKLLGIVPSIAYNFILPTWFAMVALGAFTVG
                     YGAVASSKADGEADEPSMFNLQLLAGLAGALLTVFLGNLGTIQLIFNAMQRVAAPGGT
                     VPTETGFIQHWVWAFQGIWKMITEKAMLPIGRGDWYWFPSRVIPTGPGNEITEFPLFT
                     FLYSDLHAHMLVMPLTLFIIAWALAFVRSRAQLTRGEWIASFGIGALMIGALKPTNTW
                     DLYTYYLLAAIAVGYTIFRYFDWKDYFNVPAVAGKAGLAVGAAVLLYVLGALFYLPFS
                     HWFGQSYNSFAFWTASRTSLSSYFTQWGLFLFIITAWLIWETREWMAATPISDLKKLQ
                     RYVVAIEVV"
BASE COUNT          277 a          322 c          366 g          413 t
ORIGIN      
        1 gcacttatgg catggctggc gtggatgggc ggctcgattg gtgtgcccta taccagactc
       61 agcatcggcg tggcatttac tttgatcgta gtggcaggca tcggcttatg gatgaagcgc
      121 aaggaagagt tcaaagcaga ttgggaccgc catcgtcagt tctttgtttt ggttgagatc
      181 gtcttcttaa ccttcttcct gatcgatttg ctcattcgca ttggcaaccc agatatgtgg
      241 catccatcca aaggcggcga acgcccaatg gatttctcgt atttcaatgc ggttttgaag
      301 agcactagct tcccacccta tgacccgtgg tatgccggtg gctatatcaa ttattactat
      361 tatgggtttg tgttggcggg cacgccggtg aaattgcttg gcattgtgcc gtcgattgca
      421 tataacttta ttttgcctac ttggtttgcc atggtggcgt tgggcgcttt cacggttgga
      481 tacggcgctg ttgcaagcag caaagcggat ggggaagcag acgaaccatc tatgttcaac
      541 ttgcaactct tggctgggct ggcaggcgca ttgctcacgg tcttccttgg aaatttaggg
      601 acaatccaac tgatcttcaa tgcaatgcaa cgtgttgctg ccccaggtgg gacggttcct
      661 actgagacag gttttatcca gcattgggtg tgggcattcc aaggcatttg gaaaatgatc
      721 accgaaaaag cgatgttgcc catcgggcgc ggagattggt attggttccc aagccgcgta
      781 atcccaaccg gacccggtaa cgagattaca gaatttccgc tgttcacttt cttatacagt
      841 gatttacatg ctcatatgct ggtgatgccg ttgaccttgt tcatcattgc atgggcattg
      901 gcatttgtgc gctctcgtgc gcaattgact cgcggtgaat ggattgcttc ctttggtatt
      961 ggcgctttga tgatcggcgc actcaaaccg accaatacat gggatttgta tacctattat
     1021 ttattggcag ccattgcagt ggggtatacc atcttccgct actttgattg gaaagattac
     1081 ttcaatgtac ctgccgtggc tggcaaggct ggcttggcag tgggtgctgc ggtacttttg
     1141 tatgtgcttg gcgcgctttt ctatttgcca ttctcgcatt ggtttggtca gagctacaac
     1201 tctttcgctt tctggaccgc cagccgaact tcgttgtctt cctacttcac ccaatggggt
     1261 ctgttcctct ttatcatcac cgcctggctg atttgggaaa cccgcgaatg gatggctgct
     1321 actcccattt ctgacctgaa gaaattacag cgttatgtgg tcgccatcga agtggtga
//
LOCUS       DAOREF010000897         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_94321_length_1377_cov_3.576399, whole genome shotgun sequence.
ACCESSION   DAOREF010000897 DAOREF010000000
VERSION     DAOREF010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94321_length_1377_cov_3.576399"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..631
                     /locus_tag="PKI33_15420"
     CDS             95..631
                     /locus_tag="PKI33_15420"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNM38459.1"
                     /translation="MTTIQTKTEIISALQGSNKHMQEWFSAISAIEYFTRQGDTWSAA
                     DNVDHVIKAVKPIVLALKLPRVVLKIFFGRPQHSSRPYKEVCRIYSEAISKGAKASGQ
                     FLPSENPVQDKERAKTMQLQKAEKIIQDLISALEKWEDSTLDQCQLPHPIIGNLTMRE
                     MLFFTIHHNVRHASHEGD"
     gene            741..>1377
                     /locus_tag="PKI33_15425"
     CDS             741..>1377
                     /locus_tag="PKI33_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38460.1"
                     /translation="MKIFKYWASEKVRTDVYGEIKEFNIYGGSNISTADAAQNAIQKA
                     ELIKRKIEGDRSAFEEYEVDIREEILQVVDDKNIITRNRYGAQVLNTEYMLILDIDKP
                     KPAGLMGLFKKASGENDKDKIFNMVRTQAASPKYAGLGFRLYETYQGARVIVTGRDFN
                     ARDSSTFELMKDFNTDPLYAAICRKQGCFRARLTPKPNRVQMKAYKVKYPRS"
BASE COUNT          457 a          252 c          292 g          376 t
ORIGIN      
        1 attaattatt ggcagggctg gtatttgcca gccctggctg tatttaagaa acttcttgtt
       61 gatttaatgg tttaaaccgt aaaggaaaaa ctttatgaca accatccaaa ccaaaactga
      121 gattatttcc gctttgcaag gctcaaataa gcatatgcaa gaatggtttt ctgctatttc
      181 tgctatagag tatttcacgc gccaaggaga tacttggtct gccgctgata atgttgacca
      241 tgtcattaaa gccgtgaagc cgattgtgct agctttaaag ttaccgcgcg tggtattgaa
      301 aatcttcttt ggaagaccac aacattcttc gagaccctat aaagaagtat gcagaattta
      361 ttcagaagca atttcaaaag gagcaaaagc ctcagggcaa ttcctgccaa gcgaaaaccc
      421 tgttcaagat aaagaacggg ctaaaaccat gcaattgcag aaggcagaaa aaattattca
      481 agacttaatt tctgctttag aaaaatggga agatagcact cttgatcagt gtcagttacc
      541 ccatccgatt attggaaacc tgaccatgcg agagatgctt ttctttacca ttcatcataa
      601 tgtacgtcat gcaagccatg aaggagacta gtatctggaa catcctccgt atccccaata
      661 tggaggatgt ttttatttaa gaaccgccca tataattttt ttgagtttta tgtagatttg
      721 atgaacaaag gagaaaatat atgaaaatat tcaaatattg ggcttcagaa aaagtacgaa
      781 cagatgttta cggggagatc aaagaattca atatctatgg cggctcaaat atctcaacag
      841 ctgatgctgc gcaaaatgca attcaaaaag cggagttaat caaaagaaaa attgaagggg
      901 atagaagcgc ttttgaagaa tatgaagtgg atattcgcga agagatactt caagtagtgg
      961 atgataagaa cattattacc cgtaatcgat atggcgctca agtcttgaac acggaataca
     1021 tgcttattct tgatatcgac aaaccaaaac cagccggttt aatggggctt tttaagaagg
     1081 cgtcgggtga aaatgataaa gacaaaattt tcaacatggt gcgaactcag gcagctagcc
     1141 caaaatatgc ggggctaggc tttcgccttt atgagactta tcaaggtgca cgtgtaatcg
     1201 taaccggtag agactttaat gcccgtgata gttctacatt tgaactgatg aaagacttta
     1261 atacagaccc tttgtatgca gcgatctgcc gtaaacaggg atgttttcgt gccagactga
     1321 ccccaaaacc aaatcgagtt caaatgaagg cttataaggt gaaatatccg cgtagtg
//
LOCUS       DAOREF010000898         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_94448_length_1376_cov_3.582892, whole genome shotgun sequence.
ACCESSION   DAOREF010000898 DAOREF010000000
VERSION     DAOREF010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94448_length_1376_cov_3.582892"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..628
                     /locus_tag="PKI33_15430"
     CDS             140..628
                     /locus_tag="PKI33_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38461.1"
                     /translation="MLSKFIAFLKDLLFIPVVLGAIALFGQSLITTERVTCELQEDQF
                     YSCTAQDLIFGLRISERTAEKVHSIGYDLKCSESSDGRSCTAFASFKTEDGTSVQVSK
                     RYKDPDQVRKMTEMLNSLINEKSTSINITFPPSLFATILAGLFAVGSSFFIISNALEN
                     FK"
     gene            612..>1376
                     /locus_tag="PKI33_15435"
     CDS             612..>1376
                     /locus_tag="PKI33_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38462.1"
                     /translation="MKISNNKKTLLMPTLEQLLRLPNIDSGLPFSISPDETRIVFSWN
                     KTGSWELWEHKDGALELVKIELEGAKFSPHFSPDGTKLAFALDPDGSESYHICVHDLI
                     TNTTTDLTPGILYANQPNMSWAPDGQSLAVLSEAKGIFSLYVLPLDDSGLHMLKNVFR
                     PCWDAAWSPDGMWIAIEAEAEASDRNIYIVPVGRRKGPRVGTTHLKVDGKALNAMHPA
                     WSPDSKSLAFSCENGEWFNIGLYHVETKEVAWATDSI"
BASE COUNT          407 a          325 c          293 g          351 t
ORIGIN      
        1 ggtagctcgc cttcactcat acatgctcga cctctccaag aaagcaggcg ttgaagaatt
       61 accaaaactg gaagattacg aactggcata aacgtgtcgg attaataata taccaaaggc
      121 aaagtataga gagtatttaa tgttaagtaa atttattgca ttccttaaag accttctgtt
      181 tattcccgta gttttaggtg ctattgcttt gtttggtcaa tctctaataa ccacagaacg
      241 ggttacttgt gaattacaag aagatcagtt ctatagttgc accgctcaag accttatatt
      301 tggattaaga atttcagaaa gaacagcaga aaaagttcat agtattggtt acgatctcaa
      361 atgcagtgaa agtagcgatg ggcgcagttg caccgccttt gcctcattca aaaccgaaga
      421 tggcacttcc gttcaagtaa gtaagcgata caaagatcca gaccaagtac gcaaaatgac
      481 agaaatgcta aactctctca taaatgaaaa aagcacttcg attaatataa cctttccacc
      541 ttcgctcttc gccacgatct tagcaggtct ttttgcggta ggttcatcct tcttcatcat
      601 aagtaatgca cttgaaaatt tcaaataaca agaaaactct cttaatgcca actctcgaac
      661 agcttctacg cctccccaac atagacagtg gtctgccatt tagcatttcg ccagacgaaa
      721 cacgaatcgt attctcttgg aacaagaccg gctcatggga gttatgggaa cacaaagatg
      781 gagcactcga actagtaaaa attgaactgg aaggcgcaaa attctcccct cacttttcgc
      841 ctgacggtac caaactcgcc tttgctttag acccagatgg gagtgagtcc tatcacatct
      901 gcgtccacga tctgataaca aacaccacca ccgatctgac accaggcatt ttatatgcca
      961 accaaccgaa tatgtcttgg gcgccagatg ggcaatctct agctgtctta tccgaagcca
     1021 agggcatctt ctcgttatac gtactgccgc ttgacgacag cgggttgcat atgctcaaga
     1081 atgttttccg cccatgctgg gacgcagcct ggtcaccaga tgggatgtgg atcgcaatcg
     1141 aagccgaagc tgaagccagc gaccgcaaca tttacatcgt gccggttggg cggcgcaaag
     1201 gtccacgggt tgggacgact cacctaaagg tcgacgggaa agccctaaat gccatgcatc
     1261 cggcatggtc accagactca aagtctttgg cgttttcttg tgaaaatggc gaatggttca
     1321 atattggctt gtatcatgta gagaccaaag aagtcgcttg ggcgacagac tctata
//
LOCUS       DAOREF010000899         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_95018_length_1372_cov_3.029613, whole genome shotgun sequence.
ACCESSION   DAOREF010000899 DAOREF010000000
VERSION     DAOREF010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95018_length_1372_cov_3.029613"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKI33_15440"
     CDS             <1..753
                     /locus_tag="PKI33_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM38463.1"
                     /translation="RAQKELLERKRVEAERERIIGELEMKNAELERFTYTVSHDLKSP
                     LITIGGFIGLLEKDTLNGDVNRLKNDLEKIREAKDKMARLLDELLELSRIGRVKNPSG
                     EFSLKQIVDEAVTLVQGRITSGRVQVIIHEPLPMLYCDGPRLVEVMQNLLDNAAKFMG
                     DQPTPRIDISARNDADEIIVYIKDNGMGINPIFHTKIFNLFDKLDPKSEGTGAGLALV
                     KRIIEVHEGRIWVESDGAGTGSCFCFTLPAKT"
     gene            874..>1372
                     /locus_tag="PKI33_15445"
     CDS             874..>1372
                     /locus_tag="PKI33_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNM38464.1"
                     /translation="MHEITLIINIVVALVVAFIGGVIARRLGLPTIVGYLFAGIAIGP
                     FTPGFVGDTHTISQLAELGVIFLMFGVGLHFSLSDLWKVRSIAIPGALGQMALTTLLA
                     FFLSRSWGWNASAGIVLGLAISIASTVVLLRGLMDKGLLNTAHGQAAVGWLVLEDLAT
                     VLILVL"
BASE COUNT          357 a          274 c          366 g          375 t
ORIGIN      
        1 cgagcgcaaa aagaattgct tgagcgcaaa cgagttgaag cagaacgcga aagaatcatt
       61 ggtgagcttg agatgaaaaa tgcggagtta gagcgtttta catacaccgt ctctcatgac
      121 ctaaaaagcc cgctaatcac tatcggcggc tttatcgggt tactcgaaaa agacacgctt
      181 aatggagatg taaatcggct aaagaatgat ttagaaaaga tccgcgaagc aaaagataaa
      241 atggcgcgtc tgttagacga gcttttggag ctctcacgta ttgggcgagt gaaaaaccca
      301 tctggtgaat tttcattgaa acagatcgtc gatgaggcgg ttacattggt tcaaggcaga
      361 atcacatctg ggagagtgca agtgataatt catgaacctt taccaatgtt atattgtgat
      421 ggtccgcggc tggtagaggt aatgcaaaat ctattggaca atgccgcaaa atttatggga
      481 gaccagccca caccgcgaat tgacatcagc gcaaggaatg atgccgatga aattatcgta
      541 tacattaaag acaatggcat gggtattaat cccatttttc ataccaagat ctttaattta
      601 ttcgacaagc tggacccaaa aagtgaaggg actggtgccg ggctggcgct ggtcaagcgc
      661 atcattgaag tccatgaagg tcggatctgg gtcgagtcag atggtgccgg tacaggtagt
      721 tgtttctgct ttacattacc cgctaaaacg taacctcaaa tgatataagg taataggttt
      781 attaaaaatc cgcattccag actggaatgc ggattttttg tgtacaatga ttttgtcgaa
      841 ccgagagcta acttaatagt ttatggagga aatatgcacg aaattacgtt gatcatcaat
      901 attgttgttg cgttggtggt ggcttttatt ggcggagtca tcgcgcggcg gcttgggctg
      961 cctaccattg tgggatattt atttgcaggc atcgccatcg gacctttcac cccgggtttt
     1021 gtcggagata cgcatacgat cagtcagctc gccgagttgg gagtcatctt cctgatgttt
     1081 ggtgttgggc tgcacttttc actgagcgat ctttggaaag tccgttccat cgccatccct
     1141 ggcgcattgg ggcaaatggc gcttactacg ctgttggcat tttttcttag ccgatcctgg
     1201 ggctggaatg catcagcggg cattgtgttg ggtctggcaa tttccattgc gagtacggtt
     1261 gtgttgttgc gcggattgat ggataaaggc ttgctcaaca cggcacatgg gcaggctgcc
     1321 gtcggctggc tagtgttgga agaccttgcc acagtcctca ttctggtgct ga
//
LOCUS       DAOREF010000900         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_95415_length_1369_cov_3.108828, whole genome shotgun sequence.
ACCESSION   DAOREF010000900 DAOREF010000000
VERSION     DAOREF010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95415_length_1369_cov_3.108828"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /gene="hisS"
                     /locus_tag="PKI33_15450"
     CDS             complement(<1..1215)
                     /gene="hisS"
                     /locus_tag="PKI33_15450"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNM38465.1"
                     /translation="MANKIQAVKGTREFYPEQMALRNFIYSKVRSAAHAFGYQEWDAP
                     FIETIDLYAAKSGEELVKKQSFTFEDRGGDFVTLRPELTPSLARMIAAKQGELTFPVR
                     WWSFGPFWRYEQPQKGRSREFFQWNVDMLGVNSPEADAELIAVGATFLRSVGLSPERA
                     LIYVNNRRLMDSEFDALGISAEKRLDVSNLVDRRTKMEPAKWDAYALDLGLNQTQLEG
                     LKSILGNFELWKKSDELVRLFATLEALGVKEYVKFDPNIMRGLLYYTGTVFEAFDVTG
                     SLKRAIFGGGRYDNLLADVGGQPLSGVGFAMGDVVIGIILQEAGLLPEFIPSPAQVLV
                     TVFDERLWMQSFSLAAELRAAGLNVMVSPEPTKLPKQFKFADKMKMKIALVIGPDEAA
                     QGLVVVKNLVNGE"
BASE COUNT          381 a          385 c          287 g          316 t
ORIGIN      
        1 ctcgccattc acaagatttt tgacgaccac caagccttgc gccgcctcat ctgggccaat
       61 aaccagcgca attttcatct tcatcttgtc tgcaaattta aactgcttag gtagttttgt
      121 cggctctggt gagaccataa cattgagtcc cgccgcacgt aattctgccg caagcgaaaa
      181 agactgcatc cacaatctct cgtcaaagac cgttaccaac acctgtgccg ggctgggaat
      241 gaattcaggc agcaaaccag cctcttgcaa aatgatgcca atgaccacat cgcccatggc
      301 aaagcctacg ccagaaagag gttgcccacc cacatcggca agcagattgt cgtagcgccc
      361 gccgccaaag atggcgcgtt tgagtgaacc ggtcacgtcg aaggcttcga agacggtgcc
      421 ggtgtaatag agaagtccac gcatgatgtt cgggtcaaat ttgacatatt ctttgacacc
      481 caatgcttcc agtgtcgcaa agagacggac caactcatca ctcttcttcc acagctcaaa
      541 attaccaaga atagacttca agccttctag ttgagtttga ttcaagccaa ggtcaagggc
      601 gtacgcatcc catttggcgg gttccatctt tgtgcggcga tccaccaaat tagaaacatc
      661 gaggcgtttc tcggcagaaa tgcctaaagc gtcgaattca gaatccatca ggcggcgatt
      721 attcacatag atcagagccc gctccgggct gagtccgacc gagcgcaaga acgttgctcc
      781 gacggcgatc agctctgcat ccgcttctgg cgagttgact cccaacatat ccacattcca
      841 ttgaaagaac tcacgcgagc gccccttttg aggctgttca taccgccaaa acggaccgaa
      901 cgaccaccaa cgcaccggga aagttaactc cccttgtttt gccgcaatca tacgcgctaa
      961 agatggagtc agctcggggc gcaaggtcac aaaatcgcca ccacggtctt caaacgtgaa
     1021 agactgcttc ttaaccaact cctcgcccga tttcgccgca tacagatcaa tcgtctcgat
     1081 aaatggcgca tcccattcct gataaccaaa agcatgcgca gcagaacgaa ccttactgta
     1141 gataaagttt cgtaacgcca tctgctctgg gtaaaattct cttgtacctt ttactgcctg
     1201 aatcttgttt gccatgtata ctccaaatgc cacgcatttt aacatgactc attccaggat
     1261 taatcgtaaa taagcatttc aaaattcacc cactgaattt tggtataatc ccaacatcaa
     1321 cttcaaggag agatgatatg aaaatcgact atcaggaacc cagcaaaga
//
LOCUS       DAOREF010000901         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_95473_length_1369_cov_2.133942, whole genome shotgun sequence.
ACCESSION   DAOREF010000901 DAOREF010000000
VERSION     DAOREF010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95473_length_1369_cov_2.133942"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKI33_15455"
     CDS             <1..180
                     /locus_tag="PKI33_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38466.1"
                     /translation="RCIVIEDSIPGVTAGIRAGMRVYGHAAFTAAEKLKDAGAIPFGD
                     MYQLKEMIATEYSHS"
     gene            complement(210..1022)
                     /locus_tag="PKI33_15460"
     CDS             complement(210..1022)
                     /locus_tag="PKI33_15460"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNM38467.1"
                     /translation="MAEIKMAVGETTATIKHSRHIQFSGTKNFRDLGGYSTVDGRTVR
                     WGVLYRSDSLHNLTTSDLQRLASLKLSRVFDFRSENEVTREPDRLPEGAKLISLPMLD
                     ASTKIWHEQRKEMIKNMRTLKPAEFLRATNSELATKFTPEYKFFYRELLASNGDPILF
                     HCAAGKDRTGFAAATILRILGVPQDIIMQDYLLTNRYLLDAFKRNLFIGTILKGKRFT
                     DGIRGFMMADESYLNTAFETLEKEYGSFKNYVRNGLGLTDQDIERLKTYYLE"
BASE COUNT          429 a          299 c          249 g          392 t
ORIGIN      
        1 cgttgcatcg taatcgaaga cagcatcccc ggagtaacgg cgggaatccg cgcaggaatg
       61 cgagtatatg gacatgcagc gtttactgca gcagaaaaat tgaaagacgc tggggcgatt
      121 ccatttggcg atatgtatca attaaaagaa atgatcgcaa cagaatatag tcactcataa
      181 caaaaagacg aggttacctc gtctttttgt tactctaaat aatatgtctt caaacgctca
      241 atatcttgat ctgtaagacc taagccattg cggacataat tcttaaaaga cccatattct
      301 ttttcaagag tctcaaatgc agtatttaga tagctctcat ctgccatcat aaaaccgcga
      361 attccatctg taaacctttt ccccttcaaa atagtaccta tgaacaaatt ccgcttaaat
      421 gcatccaata aataacgatt cgtcaataaa taatcttgca taataatatc ttgtggcacg
      481 ccaagaatac gaagtatagt tgcggcagca aacccagtac gatcttttcc agcagcacaa
      541 tggaacaaaa tcgggtcacc atttgatgct aacaattccc ggtagaaaaa tttgtattca
      601 ggtgtaaatt tggtggctaa ttctgaattt gtagcacgca aaaactccgc cggtttgagt
      661 gttctcatat tctttatcat ttcttttcgt tgctcatgcc atattttcgt gctagcatct
      721 aacatcggca aagatatcaa tttagcccct tcgggtaacc ggtccggctc tcgagtcact
      781 tcattttcag agcgaaaatc aaatacacga gacaatttca aagaagctaa tctctgtaaa
      841 tcactggttg tcaagttatg caagctatct gaacgataca gaacacccca acgaacagtt
      901 cttccatcaa cagtggagta cccgcccaaa tcacgaaagt tcttggtgcc agaaaactgt
      961 atgtgcctgg aatgttttat tgttgcagta gtctctccta ctgccatttt aatttcagcc
     1021 atcaaaactc cttttcaaaa gaatttttat tatatcggtc aggctaagcc tagcattaac
     1081 aaccctttat gttctaacaa atatttgctt aatagcaggc tataattttg cttcttatcg
     1141 ttatacaaac tatttttata ttttggagaa agaaatgaca acacaaattt ccatgcaaga
     1201 acttgaaata ggattacctc ttatttagca atccccaaag caagaaggca aactggagct
     1261 aattgttcgc agaccttcag tggaggagcg tgaaatcctc tctcaaggca aagtggattt
     1321 aaccactggc ttggaaggcg ataactggcg cgctcgcggc agcgccagt
//
LOCUS       DAOREF010000902         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_95687_length_1367_cov_3.149390, whole genome shotgun sequence.
ACCESSION   DAOREF010000902 DAOREF010000000
VERSION     DAOREF010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95687_length_1367_cov_3.149390"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PKI33_15465"
     CDS             complement(<1..1181)
                     /locus_tag="PKI33_15465"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNM38468.1"
                     /translation="MQILNACIDAVEPGNAIRRFVQREKNLLFIDNKKYDLNEFGNVT
                     VLGLGKATEAMSLALLDVLGSYPSRGLLIPKQAFASPASEFTVSPGGHPVPSEASVRA
                     GNAAFNLASSLTERDLLICLISGGGSALMASPRHGLQLADLQTLTKLLLACGARVDEI
                     NTLRRHLDRVKGGGIAKSANRARIASLILSDVVGNPLEAIASGPTALDPSTKEDALRI
                     LKKYDLLGKVPAAILEVLENSPETPKPDDPLFDRVQNVLVGSNSLAAQAGLEMAKSLG
                     FHTHFLGDTWQGEARDVAKELCGHFNLNYERPFCLIVGGETTVTLHGHGKGGRNQELA
                     LAAAIEMNGLENSMLITLATDGEDGPTDAAGAVVTGETCQRGERLGVDAQAFLSQNDA
                     YS"
BASE COUNT          306 a          331 c          401 g          329 t
ORIGIN      
        1 gaatacgcat cgttctgact caggaaggct tgagcatcca ctccaagcct ctcccctcgc
       61 tgacaagtct cccctgtcac cacagcaccc gccgcgtcgg tgggaccgtc ttcgccgtcg
      121 gtggcgaggg tgatgagcat ggagttttcg agtccgttca tttcgatggc ggcggcgagg
      181 gcgagttctt gattacgccc gcctttgcca tgtccgtgca gggtgacggt cgtttctcca
      241 cctacgataa ggcaaaatgg acgttcgtaa ttgaggttga aatgtccaca cagttctttg
      301 gcaacgtcgc gggcttcgcc ttgccaggtg tcaccgagga agtgagtgtg aaaccccagc
      361 gactttgcca tttctaaccc ggcttgggca gcgagtgagt tgctgccaac taaaacattt
      421 tgcactcgat caaataatgg gtcatccggc tttggcgttt caggagaatt ttctaacact
      481 tccaaaattg cggcagggac tttccccagt aagtcatact tcttcaaaat cctcagcgcg
      541 tcttctttcg tggatgggtc tagtgcggtg ggacctgagg ctatcgcttc gagcgggttg
      601 ccaacgacat cggagagaat caggctggca atccgcgccc tattcgcaga cttggcgata
      661 ccgccgcctt ttacacggtc aagatgacgg cgcagggtgt tgatttcgtc cacgcgggcg
      721 ccacaagcga gcaataattt cgtcagcgtt tgaagatcgg cgagttgaag tccgtgtcgc
      781 ggggaagcca ttaacgctga gccaccgccg gagatgaggc aaatgagcaa gtcacgttcg
      841 gtgagggaag aggcgaggtt gaaggcagcg tttccggcgc ggacactcgc ttcgcttggg
      901 acgggatgtc cgccaggcga gacggtgaat tcgctggcag gggaagcaaa ggcttgtttt
      961 ggaatgagca agccgcggct gggataagag ccgagtacat ccaacaaggc aagtgacatg
     1021 gcttctgtcg ctttgcccaa gccaagtacg gtcacattgc caaactcatt caaatcatat
     1081 tttttattat caatgaataa taaattcttt tcgcgctgga caaaacgtcg aatggcattg
     1141 cctggttcga cggcatcgat gcaggcattt agaatctgca tgatatcgtt tccgcgggga
     1201 tgagtttgca gcgtgacagt ggaaaaattg cgcatgtttc gagtatgata acgcagaagg
     1261 agatgcaatg cgacccaagg tatttgtttc gcgcaagatc cgtgatgcag gtttgaattt
     1321 ggtgcttgag ttttgcgatg cggaggtgtg ggatgaagag atgccgc
//
LOCUS       DAOREF010000903         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_96269_length_1363_cov_4.825688, whole genome shotgun sequence.
ACCESSION   DAOREF010000903 DAOREF010000000
VERSION     DAOREF010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96269_length_1363_cov_4.825688"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKI33_15470"
     CDS             <1..576
                     /locus_tag="PKI33_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38469.1"
                     /translation="KNGEDITSKLTNPAGFVLVDGKYGLPYPELAQTPDAWPSQYQNP
                     TYKNVFAAGIAFAPPGPISKPFTNKNSLLIAPAPPRTGMVSGVIGRVVALNIADLIEK
                     GEMTHSEPMTEMVAACIASMGDSLWDGNAVSIVMKPVVPDRKKYANEFGRDLFTSHLE
                     MGVSGAWMKFMLHHTFIWKFKARPGWTIIPE"
     gene            587..712
                     /locus_tag="PKI33_15475"
     CDS             587..712
                     /locus_tag="PKI33_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38470.1"
                     /translation="MSSQEFSKAEKFWMNFKLYQLWRFVVLNVKILMTVYQSKRS"
     gene            958..>1363
                     /locus_tag="PKI33_15480"
     CDS             958..>1363
                     /locus_tag="PKI33_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNM38471.1"
                     /translation="MDPITLSRWQFGLTTVYHFLFVPLTLGLSWFVAYFQTRYYQTKD
                     ETYRKMTKFWGKLFLINFAIGVVTGIVQEFQFGMNWSEYSRFVGDIFGAPLAVEALMA
                     FFLESTFLGVWIFGEGKVSEKAHLASIWLAAIG"
BASE COUNT          346 a          384 c          285 g          348 t
ORIGIN      
        1 aagaacggcg aagatatcac ttccaaacta accaaccccg ccggctttgt attggtggat
       61 ggaaaatacg gtcttcctta ccccgagctt gctcaaaccc cagatgcctg gccctcccag
      121 tatcagaatc ccacctataa gaacgtattt gccgcaggca ttgcctttgc ccctcccggg
      181 ccgatctcca aaccgttcac caacaagaac agtctgctca ttgcccctgc cccaccgcgc
      241 accggcatgg tcagcggtgt gatcggacgt gtcgtagcgc tcaacatcgc agacttgatc
      301 gaaaaaggcg aaatgaccca cagcgaacct atgaccgaaa tggtcgccgc ctgtatcgcc
      361 agcatgggcg actcgctctg ggatggcaac gccgtctcca ttgtgatgaa acccgttgtg
      421 ccagaccgca aaaaatacgc caacgaattc ggtcgtgacc tgttcacctc tcacctcgaa
      481 atgggcgtca gcggcgcgtg gatgaagttt atgctgcacc acaccttcat ctggaagttt
      541 aaagcccgcc ccggctggac catcatccct gaataggagc cttgccatgt cttcacaaga
      601 attctcaaaa gccgaaaagt tttggatgaa cttcaaactc tatcaacttt ggcgttttgt
      661 ggtcttaaac gtcaaaatct taatgacggt ttatcagagc aaacgctctt aaacattctc
      721 ccctcttgtt aaaagcgcca agccttggtt tggtgctttt aattttgccc aaaacttgca
      781 acaaataaat tacataacaa aataataaca ttacaatagt caagccatgt ttgtgacatt
      841 attcacgccc acctcataag tttaatgttt acaataatgg taatcgttga ccaaagtagt
      901 caaggtttcg accaatttgg ataaagttag ccgattattt ttttggagaa cacatctatg
      961 gatccaatca ctctctcacg gtggcaattc ggtctcacaa ccgtttacca cttcctcttc
     1021 gtccccctca ccctcgggct ttcctggttt gtagcgtact tccaaacccg ctactaccaa
     1081 accaaggatg agacctaccg caaaatgacc aagttctggg gaaaactctt cctcatcaac
     1141 tttgccattg gcgtagtcac cggcatcgtg caggaatttc agttcggtat gaactggtct
     1201 gaatattccc gcttcgtcgg cgatattttc ggcgctccgc tcgcagtgga agcgctcatg
     1261 gcgttcttcc tcgaatcgac cttcctcggc gtctggatat tcggtgaagg caaagtttcg
     1321 gaaaaagcgc atcttgcctc aatctggctt gccgcaatcg gct
//
LOCUS       DAOREF010000904         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_96443_length_1362_cov_3.410865, whole genome shotgun sequence.
ACCESSION   DAOREF010000904 DAOREF010000000
VERSION     DAOREF010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96443_length_1362_cov_3.410865"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKI33_15485"
     CDS             complement(<1..844)
                     /locus_tag="PKI33_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM38472.1"
                     /translation="MFDLPDNEILPLSKEHVFWTWSAQAKVNPIAVKRAKGVYFWDVD
                     DKRYLDFNSMTMCVNIGHGDERIIKAMQEQAAELPYAAPGMTTKIRALASREVASVTP
                     QGKLSKVLFTLGGADANENAIKLARGYTKRHKILTRYRSYHGASAGAMAATGDPRRVA
                     WEPNLMTGVIHFLDPYRYRSTFHRTNPNISEADFARDYVNHLEEVILYEGPESIAGIL
                     MESVTGTNGIIIPPDGYMQGVRALCDKYGIVMICDEVMSGFGRTGKWFAIENWNVVPD
                     MITMA"
     gene            921..>1362
                     /locus_tag="PKI33_15490"
     CDS             921..>1362
                     /locus_tag="PKI33_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38473.1"
                     /translation="MNTFTYKRIVIIGTTSSGKSTLAEKLSTILGVNFIELDALHWEP
                     NWKEADTLVFRQRVDLATQAIGWVVAGNYSAVRDIVWNRAEVVIWLDYSFLIVFWRLL
                     TRTVRRAITQEVLWNGNRESFWWHLKLWSEESLFHWLFKTYWRRK"
BASE COUNT          354 a          344 c          297 g          367 t
ORIGIN      
        1 ttgccatagt aatcatatct ggcaccacat tccaattttc aattgcaaac catttaccgg
       61 tgcgcccaaa gccactcatc acctcatcac aaatcatcac aatgccgtat ttatcgcata
      121 acgcacgaac tccctgcatg taaccatccg gtggaataat gataccgttc gtgcctgtca
      181 cagactccat taagatacct gcaatggact cgggaccttc atacagaata acctcttcta
      241 aatgattgac ataatcccgc gcaaagtctg cttcagagat attcggattg gtacgatgaa
      301 aggtggatcg atatcgatac gggtcaagga aatggatcac acccgtcatc aaattcggct
      361 cccaagccac ccgacgcgga tctccagtgg cagccattgc accagcagag gcgccatggt
      421 acgaacgata gcgcgttagg atcttatgac gtttggtata tcctcgcgcc agtttgatgg
      481 cattctcgtt tgcatctgca ccaccaagcg tgaatagcac cttgctcaat tttccctgcg
      541 gtgtgaccga tgccacttct ctactcgcca aggcgcggat tttcgtcgtc ataccgggag
      601 ccgcataggg caactccgcc gcttgttcct gcatagcttt aatgatacgt tcatccccgt
      661 gcccgatgtt cacacacatc gtcatagagt tgaagtcaag atagcgttta tcgtctacat
      721 cccaaaaata cacgcccttg gcgcgtttta ccgcaatggg attaaccttg gcttgcgcag
      781 accacgtcca aaagacgtgt tctttcgaga gagggaggat ttcattatcg ggaagatcga
      841 acatggagaa ttccttcgca atgcgaaatt catactatgt aaaatacacg gtacgtatca
      901 cgaattataa tcccctgccc atgaacacat tcacttacaa acgtattgtt attattggca
      961 ccacatcttc aggcaaatct acgctagcag aaaaactctc tactatacta ggggtgaatt
     1021 ttattgaatt agatgctctt cattgggagc cgaactggaa agaagctgat acgcttgttt
     1081 ttcgccaaag agtagacctt gctactcagg caataggttg ggttgttgca gggaattaca
     1141 gtgctgtgcg cgatattgtt tggaaccggg cagaagttgt catctggttg gattattcct
     1201 tccttattgt attttggcgt ctactcaccc gcaccgttcg ccgcgctata acgcaggaag
     1261 ttctttggaa cgggaaccgt gagtcatttt ggtggcacct caaactctgg tctgaagagt
     1321 ctttgtttca ttggctgttc aaaacctatt ggcgcagaaa ac
//
LOCUS       DAOREF010000905         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_96778_length_1360_cov_2.513410, whole genome shotgun sequence.
ACCESSION   DAOREF010000905 DAOREF010000000
VERSION     DAOREF010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96778_length_1360_cov_2.513410"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..711)
                     /locus_tag="PKI33_15495"
     CDS             complement(31..711)
                     /locus_tag="PKI33_15495"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNM38474.1"
                     /translation="MRSLPRYFLALLAGSIIFIGLPLLGWGLGNLPAFFENPARTAYV
                     VVIFILQIFSLIYNPQVGQNQENRKSDAPRSKLDLILIQIFSLGIVILAPFSDSRSFG
                     VLNIEVIVRYLGLLIAIPGFILMQAGEKHLARQFSIEVTLQKDHKLIQSGPYKVIRHP
                     RYLGILIFFTGIALTFRSLLGIFTVLALAFVLIWRVFTEEKMMHEEFGKEWEVYQTKT
                     WRLVPYIF"
     gene            828..1163
                     /locus_tag="PKI33_15500"
     CDS             828..1163
                     /locus_tag="PKI33_15500"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNM38475.1"
                     /translation="MRRDVFQAIADPNRRAILALLSQQRLTLNGVAENFRISRPAVSR
                     HIKILKECGLVVVTPQGRERFVEARFDKLEEVSRWVEQYRQMWEEKFNRLDDLLDEMK
                     KENANAFKK"
     gene            1147..>1360
                     /locus_tag="PKI33_15505"
     CDS             1147..>1360
                     /locus_tag="PKI33_15505"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNM38476.1"
                     /translation="MPSKNKTTVKAEPGKQELYIIREFDAPRELVFRAHTDPNLFVKW
                     IGPHGYEMILKEFEPVNGGRYRYIHKD"
BASE COUNT          403 a          264 c          351 g          342 t
ORIGIN      
        1 aaatctccgc aagcagcgga gattttttat ttagaaaatg tatggaacaa gccgccatgt
       61 tttggtttga tatacttccc attctttgcc gaattcttcg tgcatcatct tttcttcggt
      121 aaagacgcgc cagatcaaaa cgaaggctaa agcaaggact gtaaaaatgc caagcaggga
      181 acgaaaagta agcgcaatgc cagtgaagaa gatgaggatg cccaaatagc gcggatggcg
      241 gattacttta tagggaccac tttgaataag tttgtgatct ttttgcaaag tgacttcaat
      301 gctgaattgc ctggcgagat gtttttcacc tgcttgcatg aggatgaaac cagggatggc
      361 gatcagcaag ccgagataac gaacaatgac ttcaatgttc agtacgccaa atgaacggct
      421 gtctgaaaat ggagcgagga tgacaatgcc aaggctgaag atctgaatca agatcaagtc
      481 cagtttgctg cgaggggcat cactctttcg gttctcctga ttttgcccca cttgcggatt
      541 gtagatgagg gaaaagattt gcaggatgaa aatgacaacg acatacgcgg ttcgggctgg
      601 gttctcaaag aaagctggta ggtttccgag accccaacca agcaatggaa gaccgatgaa
      661 aatgatagag cctgcaagca gggcgaggaa atagcggggg agtgatctca tattgtttta
      721 ttcctattgg atttgctgaa agtataaatg attccctctt gacaatacag aaaatttgtt
      781 ctaaaatctg gttatattat gtaactaaat ggttacgaaa aggaattatg agacgagacg
      841 tatttcaagc cattgcagac cccaaccgtc gtgccatttt ggcgctgctg tcacaacaac
      901 gattgaccct gaacggcgtt gccgagaatt ttcgcatcag ccgcccggct gtttcgaggc
      961 atatcaagat attgaaagag tgtgggttgg tggtcgtcac cccgcaagga cgtgagcgct
     1021 ttgtggaagc gcgttttgat aaattggaag aagtgtctcg ttgggtggag caataccgcc
     1081 aaatgtggga agagaaattt aatcggttgg atgatttgtt ggatgaaatg aaaaaggaga
     1141 atgccaatgc cttcaaaaaa taaaacaact gtcaaggcag agccaggtaa acaagaactg
     1201 tatatcatcc gtgagttcga tgcgccgcgc gaattggtgt tccgtgccca cactgaccca
     1261 aatcttttcg tcaaatggat cggaccgcat gggtatgaga tgatcctcaa agagtttgag
     1321 ccggtcaatg gtggcaggta tcgttatatt cacaaagacc
//
LOCUS       DAOREF010000906         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_98228_length_1350_cov_5.144402, whole genome shotgun sequence.
ACCESSION   DAOREF010000906 DAOREF010000000
VERSION     DAOREF010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98228_length_1350_cov_5.144402"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PKI33_15510"
     CDS             complement(<1..903)
                     /locus_tag="PKI33_15510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNM38477.1"
                     /translation="MDETGQDLLRQAQIYLKKGNNSQALNILALHLKDHPDSVEGWWL
                     LSFAVPDTKRQIDCLERVLHLQPSYVQARVRLDTLLGKPPSQPIAPPIKETPAPTSYV
                     ESKPITQPRSTPITTPPKPPVLKKEVKSKTSLSVFQIAILGFFGVIALAVLGGTMFFG
                     MRVFAEGQQTLPQAQQDSNKTEISMPATWTPTVTATTRPTLTPYPTITPLGFSKVEAL
                     PTRQASGIEAANGYYAPNFTLVDVNTNERVSLDNYRGKAVLVFFWATWCPYCNVEISA
                     VQMVHEAYSDDGLVVLAVNVGEGAD"
     gene            complement(1019..>1350)
                     /locus_tag="PKI33_15515"
     CDS             complement(1019..>1350)
                     /locus_tag="PKI33_15515"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNM38478.1"
                     /translation="WQSILPGYARCVRITRDQKESFKVDEKAFVEAEEKALFKAIQDN
                     AKSQPATVDEFLNIVVKLIPSINAFFDKVLVMAEDKKVKENRLGLLQRIANLSNGIAD
                     LSTLEGF"
BASE COUNT          342 a          327 c          322 g          359 t
ORIGIN      
        1 atcggcgcct tcgccaacat tcacagccaa aaccaccaac ccatcatctg aataggcttc
       61 atgcaccatc tgcaccgcag agatctctac attacagtaa gggcaccaag ttgcccaaaa
      121 gaacaccaac actgccttac cgcggtagtt atccaagcta actcgttcat tcgtattgac
      181 atccactaac gtaaagttcg gggcgtaata tccattcgcg gcttcgatgc cagaggcttg
      241 acgggtgggc aacgcttcca ctttggaaaa gcccagcggc gtaatggtgg gatacggcgt
      301 cagtgtaggg cgagttgtag cggtaacagt cggagtccat gttgcgggca tggagatttc
      361 cgtcttattc gagtcttgtt gcgcttgtgg cagggtttgt tgtccttctg caaacactcg
      421 cataccgaag aacatggtgc cgccaagcac tgccaaagca atcaccccaa agaatcccag
      481 aattgcaatt tgaaaaacgg aaagactcgt tttactcttg acttcttttt ttaatacagg
      541 cggttttgga ggtgtcgtta tcggcgttga cctaggctgc gtaattggct tagactcaac
      601 ataagaggtt ggagccgggg tttccttaat cggcggtgca atgggctgag aaggcggctt
      661 gcccaacaaa gtatccaagc ggacgcgcgc ttgaacatag gatggttgca aatgcaaaac
      721 tcgttccaag caatcgatct gccgtttggt atctggcacg gcaaaactta gcaaccacca
      781 accttctacc gagtcaggat ggtctttcag atgaagcgcc aagatattga gggcttgtga
      841 attattgcct ttttttagat agatttgagc ttgtcttaat aagtcttgtc ccgtctcgtc
      901 cataagaact cctatccccg ctttgaccca atcgattgaa accagcgggc aactaaatta
      961 tagaccaaaa tacatattgg tgtaataaaa aaggacgggc ttttacccgt cctttttact
     1021 agaaaccttc gagtgtgctc aagtctgcaa tgccgtttga caagttcgca atgcgttgca
     1081 gcaacccaag gcgattttct ttcacctttt tatcctctgc catcaccagc actttatcga
     1141 aaaaggcgtt aatggatggg atgagcttga caacgatgtt caagaattca tccaccgtgg
     1201 caggttgaga tttggcgttg tcttgaatgg ctttgaacaa cgctttttct tctgcctcca
     1261 caaaggcttt ctcatccact ttgaaggatt ctttttggtc gcgcgtgatg cggacgcagc
     1321 gggcatagcc gggcagaatg gattgccaat
//
LOCUS       DAOREF010000907         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_98512_length_1348_cov_4.741686, whole genome shotgun sequence.
ACCESSION   DAOREF010000907 DAOREF010000000
VERSION     DAOREF010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98512_length_1348_cov_4.741686"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKI33_15520"
     CDS             <1..510
                     /locus_tag="PKI33_15520"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38479.1"
                     /translation="LADFDGEFLKRWRVRHPEKMYVPLEERKTFYVKESYPQDLQPLV
                     SEIYLEPGFFREMMPVEGGVDAVKTMQQMGLEIFFCTSPLYIYKNCVLEKYEWVDRHF
                     GSEWVNRIVLTRDKTLVKGDILIDDKPVITGAESMPMWEHILYDRPYNQNINRKRLTW
                     GNWKSVLSL"
     gene            597..>1348
                     /locus_tag="PKI33_15525"
     CDS             597..>1348
                     /locus_tag="PKI33_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNM38480.1"
                     /translation="MTNVLRLAAFSQNGQGGNPAGVAFYDEMPSAEEMMAIAKEVGYS
                     ETAFLAKQADGWRVRYFAPELEVPFCGHATIALGAALGDRFGEGTYKLILNDSTISVR
                     ASKTDLGMASTLQSPETWSEEAPTDFVDKVLAGFHMDRNQLDPNFPIRYAGAGAKHLI
                     LFVKDRATLANMKYDFDPVRALMMQEGLITISLLWQESDSVIHSRNPFAAGGVYEDPA
                     TGAAAAALAGYLRDIGWKDSKTFTILQGEDMGV"
BASE COUNT          366 a          287 c          359 g          336 t
ORIGIN      
        1 cttgcagatt ttgacggcga atttctgaag cgttggcggg tacggcaccc ggaaaaaatg
       61 tatgtaccac tcgaagagcg caaaacattt tatgtgaagg agtcgtatcc ccaagattta
      121 caacctttgg tatcagaaat atatctggag cccggattct ttcgtgagat gatgcctgta
      181 gagggcggag tggatgccgt gaagacaatg cagcaaatgg ggcttgaaat tttcttttgt
      241 accagtccgc tttatatata caagaactgc gtattggaga aatacgaatg ggtggatcgt
      301 catttcggct cagaatgggt aaaccgtatt gtccttacca gagacaaaac cttggtcaaa
      361 ggcgatatct tgatcgatga caaaccggtt atcacaggcg cagagtctat gccgatgtgg
      421 gagcatatct tgtatgaccg tccctataac caaaacataa acagaaaaag gctaacttgg
      481 ggaaactgga agagtgtatt aagtttataa atatctagtt ttgagggtac acgaaaaaag
      541 aatgaaaaca tctaaacgaa gtactttatt cttgatgaca ctttggaggt aataagatga
      601 cgaacgtatt aagactcgcg gctttttccc agaacgggca aggtggtaac ccggcaggtg
      661 tggctttcta tgatgagatg ccttctgccg aagagatgat ggcaattgca aaagaagttg
      721 gctattcgga aacagcattt ttagcgaagc aagctgacgg ctggagagtc cgctattttg
      781 cgccagagtt ggaagtgccg ttttgtggtc atgccaccat cgcacttgga gcggcgttgg
      841 gcgaccgctt cggtgaaggg acctataaac tgatcctgaa cgacagcacc atcagcgtcc
      901 gcgcctcaaa aacggacctc ggaatggctt caacgcttca atcgcctgag acgtggtctg
      961 aagaggctcc taccgatttt gttgataaag tattggctgg ctttcacatg gatcgcaatc
     1021 aactcgaccc gaacttcccg attcgatatg caggtgcagg cgccaagcat ttgattttgt
     1081 ttgtcaaaga ccgcgccacg cttgcgaata tgaaatatga tttcgacccc gtgcgcgcct
     1141 tgatgatgca agagggtctc atcacaatca gtttattatg gcaagaatcg gattcggtca
     1201 ttcattcacg taatcccttc gcggcgggcg gggtctatga agacccggca accggtgccg
     1261 cggcggcagc gttggcggga tacctgcgcg acatcggctg gaaagattcg aagactttca
     1321 ccatcttgca gggagaagat atgggtgt
//
LOCUS       DAOREF010000908         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_98647_length_1347_cov_5.742260, whole genome shotgun sequence.
ACCESSION   DAOREF010000908 DAOREF010000000
VERSION     DAOREF010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98647_length_1347_cov_5.742260"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PKI33_15530"
     CDS             complement(<1..1334)
                     /locus_tag="PKI33_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNM38481.1"
                     /translation="MVIAAFFIWGNPGNYSDRLFVIFKDQADISDVASIKNRDERLTE
                     AYTRLTDHANKTQADLRETLDTFGVAYTPYYLENAMEVQGGTLVRLFLMTRSEVDRVI
                     PSPRLRAAPEDSPEPGFESAPSADYVGWNIQLIGADRVWEEFGVRGEGIVVGQSDSGV
                     DGNHPAIEKQYRGYNGSDDYNWFDPWDGTTSPNDEGGHGTHTMGTILGNNGIGVAPDA
                     QWIGCVNLDRNLANPALYLDCMQFMLAPFPIGGDPFVDGDPTQAAHVMNNSWGCPSIE
                     GCDPNALLYAANHLRDAGIFVVVSTGNDGPNCETVEAPLSLYDSVFSVGAIDQSGDLA
                     RFSSRGPVTADGSGRIKPDIVAPGVDVFSSLPENSYGSNSGTSMAGPHMVGVVALIWS
                     AQPELIGDIDATEQLIIDTAKPYMGDRSLGCFSGDIPSNAFGFGVVDVYEAV"
BASE COUNT          352 a          372 c          325 g          298 t
ORIGIN      
        1 tttacagctt cgtacacatc caccacgcca aagccaaagg cgttgctggg aatatcgccg
       61 ctaaaacagc ccagcgaacg atcccccata taaggcttgg cagtatcgat aattagctgt
      121 tctgtcgcat cgatatcacc gatcagctca ggctgagcag accagatcaa cgctaccacc
      181 cccaccatat gcggacctgc cattgaagtt ccgctattgg agccgtacga attttcgggt
      241 aaggatgaaa acacatccac acctggtgca acgatatcgg gtttgatgcg cccagagccg
      301 tccgcagtga caggtccgcg gctactgaac ctagcgaggt ctccagattg gtcaattgca
      361 cccacagaga agactgagtc ataaagagat aaaggagcct ctaccgtttc gcagttcggt
      421 ccgtcattgc cggtagaaac caccacaaaa atgccggcgt cacgcaaatg gtttgcagca
      481 taaagcaaag cattcgggtc acagccttca atcgaagggc agccccatga attgttcatg
      541 acatgcgcgg cttgagttgg gtcaccatct acaaagggat caccgccaat cgggaagggc
      601 gcaagcatga actgcataca atctaaatac aaagctgggt tagcaagatt acggtcaagg
      661 ttcacacacc cgatccattg tgcatcagga gcaacaccga tgccattatt tccaaggatc
      721 gttcccatag tgtgagtgcc gtgcccgcct tcatcattcg gtgatgttgt gccatcccac
      781 gggtcgaacc agttgtaatc atcactgccg ttataaccac gatactgttt ttcgatcgca
      841 ggatgattcc catctacacc cgagtcagat tgccctacta caatgccttc gccgcgcacg
      901 ccgaactctt cccagactcg atctgcgccg ataagttgaa tgttccaacc aacataatct
      961 gcggacggag cagactcaaa gcccggttca ggagaatctt cgggagcggc acgcaggcgt
     1021 gggctaggaa tgacgcggtc cacttcggag cgggtcatca agaacaggcg cacaagcgtc
     1081 ccaccttgca cttccatcgc gttctctaaa taatatggcg tatatgccac accaaatgta
     1141 tctagcgttt cacgcaggtc tgcttgcgtt ttatttgcat gatccgtaag gcgcgtataa
     1201 gcttcggtca aacgctcatc gcggtttttg atggatgcta catccgaaat atcggcttgg
     1261 tctttgaaga taacaaagag gcggtcacta taattgccgg ggttccccca gatgaaaaaa
     1321 gcggcgatga ccatgatcca cgctgcg
//
LOCUS       DAOREF010000909         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_98832_length_1346_cov_3.340046, whole genome shotgun sequence.
ACCESSION   DAOREF010000909 DAOREF010000000
VERSION     DAOREF010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98832_length_1346_cov_3.340046"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..633)
                     /locus_tag="PKI33_15535"
     CDS             complement(64..633)
                     /locus_tag="PKI33_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38482.1"
                     /translation="MKRTSKWAIGVVVTVTAAFAALQTVSAPTHQTETPPAETESPVL
                     TEVVATQPCGYQWAYQDDLELTAAFDASIKRLDPNASGRAQLFGENCVRQDGTFTFSA
                     LETDFYVRTQVADLNQEEEFGNWVKHVMEIVLQIPREELAIQYGFVEFHFEKTETEQH
                     AFRVPIKTYIDEAQSKTGAELFQMLYTQP"
     gene            complement(636..>1346)
                     /locus_tag="PKI33_15540"
     CDS             complement(636..>1346)
                     /locus_tag="PKI33_15540"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNM38483.1"
                     /translation="VDGEFIPAEKGVVLPQGGVLQTGETGRARLDLNHEGTIVRVAPN
                     SSFTLPQISEVDGAPKTSIELFFGKIFILLNGGSVDVETPSGVASVRGSLLSVSFNPA
                     TQRVQATCLEGHCGLVNASGEDVDLVEGESAYIDEDGALHELAGIDQDEIQAWLDEAP
                     ELNQFLNELPDPQDYPEFQEFDVYDFDPSAYFQENPSEADGFYSFDEGTTGSGSDPNE
                     PPATEPPSDDGSGDSSGG"
BASE COUNT          341 a          332 c          308 g          365 t
ORIGIN      
        1 atgaccgttt gatgagcttg tcaaggacaa aaaaaccgcc tttgaaggcg gtttcggttt
       61 tgattagggt tgggtgtaaa gcatctggaa taactcagca ccggtcttgc tttgtgcctc
      121 atcgatatag gttttaatcg gcactcgaaa ggcgtgttgt tcagtttcgg ttttctcaaa
      181 gtggaattct acgaacccat attgaattgc caactcttca cgcggaattt gcaatacgat
      241 ttccataacg tgtttgaccc aattcccaaa ctcttcttct tggttcaaat ctgctacttg
      301 cgtgcggacg taaaaatctg tttctagagc agagaaagtg aaggttccat cttggcgtac
      361 acaattttcg ccaaaaagct gggcgcgtcc gctggcattg gggtctaagc gtttaataga
      421 tgcatcaaac gctgccgtca attcgaggtc atcttgatat gcccattgat acccgcaagg
      481 ttgagtagca acgacttctg tgagaacagg gctttctgtt tcagcaggag gtgtttctgt
      541 ttggtgggta ggtgcagaga ctgtttgtaa ggcagcaaaa gcagctgtga ccgttactac
      601 aaccccaatt gcccatttgc ttgtgcgttt cacagctacc ctccgctaga gtcgccgctg
      661 ccatcatctg aaggaggctc agtagcaggt ggctcgttcg ggtcactgcc tgaaccagtt
      721 gtaccttcat cgaaagagta gaagccatct gcttcactgg gattttcttg aaagtatgca
      781 gaagggtcaa aatcatacac atcaaattcc tgaaactcag ggtaatcttg agggtctggt
      841 agttcattta agaactgatt caattctggg gcttcatcca accaagcttg aatctcatcc
      901 tggtcaatgc cagccaactc atggagcgcc ccatcttcat caatgtaagc agactcgcct
      961 tctaccagat caacatcttc accgcttgcg ttgacgagtc cgcagtgacc ttccaaacac
     1021 gtagcttgaa cacgttgcgt tgccgggttg aaactgacac tcaacaaact accacgtacc
     1081 gatgccacgc ccgatggcgt ttctacatca accgagccgc catttaataa gataaagatc
     1141 ttgccaaaga aaagctcaat actggtcttg ggggcgccat ctacttctga aatttgaggc
     1201 aacgtaaatg aagaatttgg agctacacga acaatggtcc cttcgtggtt tagatccaaa
     1261 cgagcccgcc cggtttctcc agtttgtaac actccgcctt gtggcaaaac aacccctttc
     1321 tctgccggga tgaactctcc atcgac
//
LOCUS       DAOREF010000910         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_99342_length_1343_cov_3.125000, whole genome shotgun sequence.
ACCESSION   DAOREF010000910 DAOREF010000000
VERSION     DAOREF010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99342_length_1343_cov_3.125000"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..902
                     /locus_tag="PKI33_15545"
     CDS             438..902
                     /locus_tag="PKI33_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38484.1"
                     /translation="MKVKSVLQSQYLAALAMFKQAVVQCPPEIWDDEQDKDKFWYVAY
                     HTLRYAHQYLKARDKGYPRWEQRPYSKPGIPFTKDEILEKLAQVERDVLEQIPLMNLE
                     EKTGATGALENKLELQIYNIRHIQQHTGELYQRLSATDAKLSWASKRTKENK"
     gene            899..>1343
                     /locus_tag="PKI33_15550"
     CDS             899..>1343
                     /locus_tag="PKI33_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNM38485.1"
                     /translation="MTKELPKAYDFKSTEPRIYAMWEAGKYFKPHNDPNQPDFDPNVK
                     PFVISIPPPNVTGELHLGHAMFVSVEDLMIRYHRMKGYSTLWVPGTDHAGIATQLMVE
                     RELLRTEEVTREEIGREKFLERTWAWKKKYGGMITNQIRRLGASCD"
BASE COUNT          399 a          322 c          320 g          302 t
ORIGIN      
        1 gctcaatttt tagatacttg ggcgcttaaa caaaattacg atcttgtaga tgaaattgtt
       61 atcgagatga acatgggagg catctatact ctacaagtat ggaagcccaa gccatgacct
      121 attggtattc ttgacgtctc ttctcctccc gcatataatc ccagtcattc agaagtagta
      181 agtagaaggt tccctgatag agaaggatgt gctcggtgga agcatccgca ggaaaacagt
      241 accgaagtcg tctgagttga cctgccgaag aaatccgcgt gaaagtagac cggcacgggg
      301 ttgcccgtta cagcaaaagc tgatggttgt cagcgaatga gtgtcccgcc cgtgcgggaa
      361 accgaggtgg taccgcggag cctacgcttc gtcctcatac aggacgaagc gtttttgttt
      421 gtcagaatgg aagaagaatg aaagtcaaaa gtgtcctcca atcccaatat cttgcggcac
      481 tcgccatgtt caaacaagct gtcgttcagt gtccgccaga gatatgggat gacgaacaag
      541 ataaagacaa gttctggtac gtggcttacc ataccctaag gtacgcacat cagtatttga
      601 aagcacgaga caaaggatac cctcgctggg agcaaagacc ttattcaaag cccggcattc
      661 catttacaaa ggatgagatt ctcgaaaaac ttgcccaagt tgaaagagat gtcttggaac
      721 aaatcccgct catgaattta gaggaaaaaa caggagccac tggtgctctt gagaataaat
      781 tagaactaca aatctataac atccgccata ttcagcaaca caccggcgaa ctttatcaga
      841 gactgagcgc gaccgacgca aaattgagtt gggcgtccaa gcgaaccaag gagaacaaat
      901 gaccaaagaa ctacccaaag cctacgattt caaatccacg gaaccacgca tttatgcgat
      961 gtgggaagct ggcaaatact tcaagccgca caatgacccg aaccagccag actttgatcc
     1021 gaacgtcaag ccgttcgtca tctctattcc cccaccaaat gtgaccggtg aactgcacct
     1081 cggtcatgcc atgtttgtga gcgtggaaga cttgatgatt cgctatcatc gtatgaaggg
     1141 ctattctact ctttgggtcc ccggcaccga ccatgcaggc attgccacac aacttatggt
     1201 ggaacgtgaa ctccttcgaa ctgaagaagt cacccgtgaa gaaattgggc gcgaaaaatt
     1261 ccttgagcgt acttgggcat ggaaaaagaa atatggcggc atgatcacga atcagattcg
     1321 ccgcctcggc gcttcctgtg att
//
LOCUS       DAOREF010000911         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_99915_length_1339_cov_3.831776, whole genome shotgun sequence.
ACCESSION   DAOREF010000911 DAOREF010000000
VERSION     DAOREF010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99915_length_1339_cov_3.831776"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1339)
                     /locus_tag="PKI33_15555"
     CDS             complement(<1..>1339)
                     /locus_tag="PKI33_15555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNM38486.1"
                     /translation="LLTPRILTLLSGLKVDLLTIDEAHCISEWGHDFRPEYRQIVDVR
                     KKFPNAVCMALTATATSRVRQDIRHTLKFATTNEFIASFNRENLFIEVVPKQDPAQQV
                     REIIDRYKDQSGIIYCFSRKQVDELAAYLTTKGYSVRPYHAGLDDAERRKNQELFIRD
                     DVQIIVATIAFGMGINKPNVRFVIHHDLPKSIEGYYQEIGRAGRDGLPAHCVLLYSYA
                     DVAKLNYFIDQKEGDEKRVAIEHLNAIVRYAEDERNCRRKPLLNYFGESYSAETCSNC
                     DNCNSAPTPLTDVTIPAQKFLSCVKRADEKFGAGHIVDILLGSKNEKVLRWEHDKLST
                     YGIGKELDRKQWMHLARQLLSMGYLKQEGEFHTLSLTPKALEALRKREPIFGVMQEAE
                     RVKKDGRKKAAELDYHRGLFAILRQKRKEMADEAGVPPYVIFSDRTLTEMAAYF"
BASE COUNT          323 a          328 c          323 g          365 t
ORIGIN      
        1 ggaaatacgc cgccatctcg gtcaaagttc ggtctgagaa gatcacatac ggcggcaccc
       61 ctgcttcgtc tgccatttct tttctttttt gacgaaggat ggcaaacagc ccgcgatggt
      121 aatccaactc tgcggctttt ttcctcccat ctttcttgac acgttccgcc tcttgcatga
      181 cgccgaaaat gggttcgcgc ttccgaagtg cttccaacgc cttgggcgta aggctcaggg
      241 tatggaactc cccttcctgc ttgaggtatc ccattgaaag gagttgacga gcaagatgca
      301 tccactgctt tctatctaat tcttttccaa tcccatacgt ggacagtttg tcatgctccc
      361 aacggagcac tttctcattt ttagaaccga gcaaaatatc tacaatatgc ccggcaccaa
      421 atttctcgtc tgcgcgtttt acgcaagaga gaaacttctg tgcgggaatg gtcacatcgg
      481 ttagcggagt cggggcggag ttacagttgt cgcaattgga acaggtttcg gcggagtatg
      541 attcgccgaa ataattgagc aacggtttgc ggcggcaatt gcgttcatcc tctgcataac
      601 gaacaatggc atttagatgc tcaatggcta cacgcttttc gtcgccttct ttttggtcga
      661 tgaaatagtt taatttggca acatctgcat agctatatag caaaacacaa tgtgcaggca
      721 gaccatcacg cccggcacgc ccaatttctt ggtaatagcc ttcaatactt ttgggcaggt
      781 catggtggat tacaaagcgc acattaggct tattaatgcc catgccgaag gcaattgtcg
      841 ctacaatgat ttgaacatca tcacgaatga ataattcttg atttttacgc cgttctgcat
      901 cgtccagccc ggcatgatac ggacggaccg aatatccctt tgtggtaagg tatgcagcga
      961 gttcatccac ttgtttacgc gaaaagcagt agataatgcc agattgatct ttataccggt
     1021 caatgatctc gcgcacttgt tgcgcagggt cttgtttggg tacaacttca atgaaaagat
     1081 tctcgcggtt aaaactggcg ataaattcgt tcgtggtggc aaacttgagc gtatgccgaa
     1141 tatcttgccg cacacgagag gtggctgtcg ccgtcaacgc catgcaaacg gcattcggga
     1201 atttcttgcg cacatccacg atctgtcggt attcggggcg aaagtcatgc ccccactctg
     1261 agatgcagtg cgcttcgtcg atggtcagca agtccacttt cagtccagaa agaagcgtga
     1321 gaattcttgg ggtgagaag
//
LOCUS       DAOREF010000912         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_102387_length_1323_cov_3.990536, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000912 DAOREF010000000
VERSION     DAOREF010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102387_length_1323_cov_3.990536"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /gene="rho"
                     /locus_tag="PKI33_15560"
     CDS             complement(<1..870)
                     /gene="rho"
                     /locus_tag="PKI33_15560"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNM38487.1"
                     /translation="MKILELENEPLSKLRMMARDLNIQNANRLKKETLAMLIREAEAQ
                     KEGIELRGGILEIMSEGIGFLRATNYRISDQDVYVSQAQLRRYDLRAGDLVIGQVRPP
                     RESERHFGLLKVESINGLDPEDAIRRTVFENLTPIFPDKRFDLETDPKTLAPRLINLI
                     APIGRGQRGLIVSPPKTGKTTILKQIANSISTKYPDVHLIIALIGERPEEVTDMDRSV
                     EAEVVASTFDEPVTSHVRTAELALDRAKRLVEIGRHVVILMDSITRLARAYNLTVNPS
                     GRTLSGGMDPSALY"
BASE COUNT          337 a          263 c          332 g          391 t
ORIGIN      
        1 gtataacgca gagggatcca taccgccgga gagggtgcgc ccagaagggt taacggtcaa
       61 gttataggca cgggcgaggc gggtgatcga gtccattaag attactacat gtcgaccgat
      121 ttccaccaat ctcttagcgc ggtctaatgc aagttctgcc gtgcggacgt gtgatgtgac
      181 cggctcatcg aaggtggagg cgaccacttc ggcttccact gagcggtcca tgtcggtaac
      241 ttcttcaggg cgttcgccga tgagggcaat aattaagtga acgtctggat attttgtaga
      301 aattgaattt gcaatttgct tcaaaattgt agttttcccc gttttgggcg gcgaaacgat
      361 caaaccgcgc tgccctctac caatgggggc aattaagtta attaaacgtg gagctaatgt
      421 tttggggtct gtttctaaat caaaacgctt atctggaaag attggggtga gattttcaaa
      481 aaccgttcta cgaatggcat cttccgggtc taagccattg atggattcta cttttaataa
      541 accgaagtgt ctttcacttt ctctgggagg gcgaacctgc ccgatcacca aatcccccgc
      601 acgaagatca taacgtctta attgagcttg cgaaacataa acatcctggt cgcttattct
      661 atagtttgtg gctcgcagaa aaccgatgcc ttcactcata atttcgagaa taccaccgcg
      721 aagttcaata ccttccttct gcgcctctgc ctcgcggatg agcatggcga gagtttcttt
      781 ctttaggcga ttggcattct ggatatttag atcccttgcc atcatacgaa gtttggaaag
      841 aggctcattt tccagttcaa gtattttcat tttcttctct ctcgacatat atgaattttc
      901 agaggcaggg gtattgggta cgctaggcgg aatggctggc gtgtttgtag attcaaccac
      961 cggggcaggc ggggtatttg aatcaaaaga attatttcta ttattgggtt tgggcgaacg
     1021 ttggtagggt cttcttgtag agttggttga acgatttgac gtatttgtag gtttatttgg
     1081 catgtgagtt gatctgttgg gatgagaatg tgaaaagacc agtccccgct tgggctggtt
     1141 aattcctaaa aggttttaat acaatgtggg atggagttca aagatatagg attcaacttg
     1201 cagacgtaga tatattgtga aatattttta ctgcgatttt tttaaaacgg caggggggaa
     1261 caatcgaatt atagcacttc catacaacag gtgcaagcaa tcaaaaaatc aattttcgtc
     1321 tgc
//
LOCUS       DAOREF010000913         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_103148_length_1318_cov_3.494062, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000913 DAOREF010000000
VERSION     DAOREF010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103148_length_1318_cov_3.494062"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1232)
                     /locus_tag="PKI33_15565"
     CDS             complement(105..1232)
                     /locus_tag="PKI33_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38488.1"
                     /translation="MTNQIEPEPIQKKKTNKWLLAFLIFLVLCAITCLILLIIRQRNS
                     GVIQAQQLALQGRCPEAIQILNEVIAENPKSVIAYQIRVECYLSFQSNVYTESQGYLI
                     AALKDTNTILDLKPQDASYYGKRDFILRELAPYEIYSANKYAIFDLANQDAQSAIELG
                     APSSAYVYRHYARNFIEANHCQEGLEETQKLIQQYGTQTSDYGQYYSLYLTEAYICLG
                     ELDLALESAQAITCDDPVSTCKSGLLAEIYLQSGDYENALNTVNYMINWQPAGGGWRY
                     FIRALIYYEQGQIDLAQQDLATGDAYSWYGTGVDEYVLAQLAFDNGDKANGIYHLQTA
                     EQMFDTNYSVLRQETLKQLKKYGAEPIPQTINLPFSTPSLP"
BASE COUNT          343 a          275 c          293 g          407 t
ORIGIN      
        1 ttccatagcc aactctcctc tcgaatcaaa atcgccccac ttccgtggga cgaccttgaa
       61 acgccaagcg agccgtttca gccgacgctg ttctagtgaa atttttaagg caaagacgga
      121 gtactgaatg gtaagttgat tgtttgtgga attggctctg cgccatattt ctttaactgt
      181 ttgagggtct cctgtctcaa gacactgtaa ttggtatcga acatttgctc ggcggtttga
      241 agatgataga tgccgttagc cttgtcgcca ttatcaaacg caagttgtgc caacacatat
      301 tcatctacgc cggttccata ccatgaatag gcatctcccg ttgccaggtc ttgctgggca
      361 aggtcaattt gtccctgttc gtagtagatc aaggcgcgga taaaatagcg ccaaccgccg
      421 cccgcaggct gccaattgat catgtagtta accgtgttga gcgcattttc ataatcacca
      481 gattgcaagt aaatctctgc caacaaacca gatttgcatg tggaaaccgg gtcatcacaa
      541 gtaatggctt gagcgctttc gagtgccaaa tccaactcac caagacagat ataagcttca
      601 gtgaggtata gactgtagta ttgaccataa tctgaagttt gtgttccgta ttgttggatt
      661 agcttttgag tttcttccaa accttcctga caatgattgg cttcaataaa gttccgggca
      721 taatgacgat acacataggc tgaactgggc gcacctaact caatggcgga ctgtgcatct
      781 tgattcgcaa ggtcaaaaat tgcatattta ttggcagaat atatctcgta aggtgcaagt
      841 tcgcgcaaaa tgaaatctct tttcccgtaa taactggcgt cttgcggttt tagatccaga
      901 atggtgtttg tatctttcaa tgcagcaatt aagtatcctt gtgattccgt gtatacgttg
      961 gattggaaac tgagatagca ctctacacgt atttggtaag caatcacaga ttttgggttt
     1021 tcggctatga cttcattaag gatctgaatg gcttcagggc atctgccttg aagtgcgagt
     1081 tgttgtgctt gaatcacgcc gctgtttctt tggcgaatga tcaaaagtat aagacaagtg
     1141 attgcacaaa gtacgaggaa aatcaagaaa gccaataacc acttattggt tttctttttt
     1201 tgtatgggtt ctggttcaat ttgattagtc atatttcctc cactattttt tgagattatt
     1261 ttggattaat tttactcatg gcatattctg ccaacgctgt gatcgttagc gacgggtt
//
LOCUS       DAOREF010000914         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_104717_length_1308_cov_5.233041, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000914 DAOREF010000000
VERSION     DAOREF010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104717_length_1308_cov_5.233041"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKI33_15570"
     CDS             complement(<1..286)
                     /locus_tag="PKI33_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38489.1"
                     /translation="MFKTKQSCKTNKILLTITLLFTLGCSFPLQVTFGTPTATPTLTP
                     TPAATQAPLSTPEPGTEQNPLILALALNPHPNDAIISAGDTLAAFIEKQTG"
     gene            334..1005
                     /locus_tag="PKI33_15575"
     CDS             334..1005
                     /locus_tag="PKI33_15575"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNM38490.1"
                     /translation="MKNTFNTIMSILILIVLAAGVYFIVQTVRETATAAQQPFEQVNQ
                     ANQALQTQVAQLANPTPTIIPDPVTYINEIRALARLETIQYSISQVMTGELNQGTFGF
                     AFGTKILVVVHGTVIAGIDMEKILPEDMRYENGVLYVRLPPAEVLATTLDTQKTEVYT
                     LQEGLLVDIDPNLVLSTIQGGQEKILSVAIEDGILQQAERNAQAYLIKFFNALGFNNV
                     IFSEE"
     gene            complement(1051..>1308)
                     /locus_tag="PKI33_15580"
     CDS             complement(1051..>1308)
                     /locus_tag="PKI33_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38491.1"
                     /translation="CNDFPQTVFFEANITANGPTLLNWQWEASTGVTSDVGTLIFEES
                     GTQVINEFYRINAPNEYWVKLKILSPNQMEEQVTFPVSCNP"
BASE COUNT          350 a          253 c          353 g          352 t
ORIGIN      
        1 aaccggtctg cttttcgatg aaggcggcga gtgtatctcc ggcagagatg atggcatcgt
       61 tggggtgcgg gtttagagct aaggcgagga tcaaaggatt ttgttcagtg cccggctcag
      121 gcgtggaaag aggcgcttgt gtggcggcag gagttggcgt gagagtagga gtcgcagtgg
      181 gagtcccaaa ggtcacttgc aacgggaaag aacatccaag cgtaaacaat agtgttatgg
      241 ttaatagaat cttgttagtc ttacaggatt gtttggtttt gaacatcatg accttgctat
      301 aatacttgaa caagatgaaa tgaaaggaag aaaatgaaaa atacgttcaa cacaattatg
      361 tccatcctga ttttgattgt gcttgctgcc ggggtctatt ttatcgtaca gaccgtgcgc
      421 gagacagcca ctgcggcgca acaacctttt gaacaggtga atcaggcgaa ccaggcattg
      481 cagactcagg tggcacaatt ggcgaacccc accccaacca tcatcccaga cccggtgacg
      541 tatatcaatg agattcgtgc gctcgctcgt ttggagacga tccaatatag tatttcgcaa
      601 gtgatgaccg gcgagttgaa tcaaggtact tttggttttg cttttggaac gaagatcctt
      661 gtggtggtgc atggaacggt gatcgcaggc attgatatgg aaaagattct gcctgaagat
      721 atgcgctatg agaatggcgt tttatatgtg cgcctgccgc ctgcagaagt attagccacc
      781 acgttggata ctcaaaagac cgaagtgtat accttgcaag aaggtttatt agtagatatt
      841 gacccaaacc ttgtgctatc cacaattcaa ggtgggcagg agaaaattct aagcgttgcc
      901 atcgaagatg gaattttgca gcaagcagaa cgaaatgctc aggcgtattt gatcaaattc
      961 tttaatgcgt tgggctttaa taatgtaatt ttctctgaag aataaagaaa acaataaaaa
     1021 aggcggctca tgtgagccgc ctttttttat ttaaggattg cagctaacgg ggaaggtcac
     1081 ttgctcttcc atttgattgg gtgaaaggat ttttagtttt acccaatact cattgggcgc
     1141 attgatgcgg taaaactcgt tgatgacttg tgtgccagac tcttcaaaga tcaatgtgcc
     1201 tacatctgag gtgacgcctg tgctggcttc ccattgccaa ttgagcaggg tcggtccgtt
     1261 ggcggtgatg ttggcttcga agaagacggt ctgcggaaag tcgttgca
//
LOCUS       DAOREF010000915         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_107237_length_1293_cov_3.793215, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000915 DAOREF010000000
VERSION     DAOREF010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107237_length_1293_cov_3.793215"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKI33_15585"
     CDS             complement(<1..622)
                     /locus_tag="PKI33_15585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNM38492.1"
                     /translation="MEYNFGLTAQDYAKHRAGFPDEFFDRVFADGIVNPTASLLDLGT
                     GTGTLARGFAARGCSVIGMDISSQLLEQAKDLSLQQGLGIEFRLGKAEETGLPDASFD
                     VVSAGQCWHWFDRPRAAQEVKRLLKPGGRVLIAHFDWLPLPGNVVDRTERLIQIYNPK
                     WYERFGNKNGSYPDWFRDLREARFEDVTSFSFDIHVPYTAEAWRGRI"
     gene            complement(626..1159)
                     /locus_tag="PKI33_15590"
     CDS             complement(626..1159)
                     /locus_tag="PKI33_15590"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNM38493.1"
                     /translation="MSITPWKILSSRHIYPRFRVDTVEVHNGTTFEPIVFEFRSWANV
                     LALTKNNEVVLVRQYRHGVQETLLELPGGVVNDGESPLDGARRELMEETGYSAEKIIE
                     VGQIYPNPAIQENTIFCYLATDVEPNGEQHFDETEEIEVVLMPLDDVIEMVRQGTFKH
                     ALHVAVLFKALMYLKKI"
BASE COUNT          345 a          354 c          275 g          319 t
ORIGIN      
        1 ggatgcgtcc acgccaggct tcggctgtgt agggcacatg gatatcgaat gagaatgaag
       61 tgacatcttc aaagcgcgct tctcgcaagt cacgaaacca atcggggtag ctaccgttct
      121 tattcccaaa gcgttcatac catttcgggt tatagatttg gataagcctc tccgttcggt
      181 cgacaacatt cccaggcagc ggaagccagt cgaagtgagc gatcaacact ctgccgcccg
      241 gcttaagcaa acgcttcact tcctgcgctg ctcgcggacg gtcgaaccaa tgccagcatt
      301 gcccggcaga gacaacatcg aatgacgcat caggtaaccc ggtttcttca gcctttccca
      361 aacgaaactc gatacccaaa ccttgttgaa ggctgaggtc ttttgcctgc tcaagcaatt
      421 gtgaagaaat atccatgcca atgaccgaac agcctcgagc ggcgaatccg cgggcgaggg
      481 tgccagtgcc cgttccgagg tccagcaaag aggcggtggg gtttacgatc ccatcagcaa
      541 agacgcggtc aaaaaattca tcagggaatc cagcccggtg tttggcataa tcttgagctg
      601 taagaccaaa gttatattcc atagactata tttttttcaa atacatcagt gctttaaaca
      661 aaaccgccac atgcaaagca tgcttgaatg tgccttgccg caccatttcg atcacatcat
      721 ccaatggcat aagcacaact tcgatctctt cggtctcgtc aaaatgctgt tcaccattcg
      781 gttcgacatc ggttgcaaga tagcaaaaaa tggtattctc ttgaatggca gggttggggt
      841 agatctgccc aacttcaata attttctctg cagaataacc tgtctcttcc attagctcac
      901 gtcgcgcccc gtccagcgga ctctcaccat catttaccac gccgccgggc agttcaagca
      961 acgtttcttg cacgccgtgc cgatactgcc ttaccaaaac cacttcgttg tttttggtta
     1021 aagccaatac attcgcccag ctacgaaatt caaagacgat cggctcaaag gttgtaccat
     1081 tgtgaacctc aaccgtatcc acccgaaaac ggggatatat atgccgcgaa ctcaaaatct
     1141 tccaaggggt aatggacatg ttttacacag cctctttatt gcatagtttt taacttctca
     1201 accaaaccat tataatttgc ccatgcctat aaccaccgac tccgtccgca cgtattacga
     1261 tgaaaacacc aacctctttt tgaaatttag cgg
//
LOCUS       DAOREF010000916         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_109034_length_1282_cov_3.691117, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000916 DAOREF010000000
VERSION     DAOREF010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109034_length_1282_cov_3.691117"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKI33_15595"
     CDS             <1..477
                     /locus_tag="PKI33_15595"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNM38494.1"
                     /translation="SITDELVQVANDNCPGQVVISGSKAAVERAMAGAKAAGAKRALP
                     LAVSIAAHSPLMASIQDEWNQAVDEASMHHPKTPVIGNVHASVLKTAEEARADIKAQM
                     QSRVKWTDSVKTISNMGVTAFVEVGTGSVLGGLVKRIVSDAVSYPLGNPQDFSALE"
     gene            561..>1282
                     /locus_tag="PKI33_15600"
     CDS             561..>1282
                     /locus_tag="PKI33_15600"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNM38495.1"
                     /translation="MNKKLAALLIFIFLTACAPLDIETPYTPVTQIPFYASDTPAPFI
                     VPVTETPQIIFPVTETPTLEQPVFVTDTPFPVAKWWEVYFTEPLTINDPNNPAGSIEE
                     KLINFINAAQVSIHIASFEFNLPRVADALIAAKARGVDVRWVTDDENGLEIDGNANRG
                     QFTRLMAAGVEVKDDAGRTALMHNKFWLFDRQITWTGSTNITVNGIYKQNNNVIVIHS
                     PEVTEIYEREWEELWTAQLGPRA"
BASE COUNT          335 a          297 c          326 g          324 t
ORIGIN      
        1 agtatcacag atgagttggt acaagttgca aatgataatt gccctgggca agtggtgatc
       61 tctggctcca aagcagcagt ggagcgcgcc atggcaggtg cgaaagctgc tggtgcaaaa
      121 cgagccttgc cattggcagt gtcaattgcg gcacactccc cgttgatggc gtctattcaa
      181 gatgaatgga atcaggctgt agatgaagcc agtatgcacc acccgaagac gccagtgatt
      241 gggaacgtcc acgcttctgt gcttaaaaca gcagaagaag ctcgagccga tatcaaagcg
      301 cagatgcaat ctcgcgtgaa atggacggat agtgtaaaaa ccatcagtaa catgggcgtg
      361 accgcttttg ttgaggtagg gacaggctct gtattgggtg gtttggtcaa gcgcattgtg
      421 agtgatgcag tcagctatcc gttagggaat ccgcaagatt tttcagcatt agaataagtt
      481 ttttacggtt aagtcactca gggtgacagt cgctttccgg tacaatacag cgactgtcac
      541 ttttgattca ggaacccata atgaacaaga aactagctgc tctcctgatt tttatcttcc
      601 ttaccgcttg tgctcctctc gatattgaaa ccccatatac accggtcaca cagattcctt
      661 tttatgccag tgatacgcct gcgcctttta ttgttccggt aactgagact ccgcagatca
      721 tttttcccgt cacagagacg cctactcttg aacaacctgt ctttgtaacc gatactcctt
      781 tccccgttgc aaaatggtgg gaagtgtatt tcaccgaacc attgacgatt aatgacccca
      841 ataaccccgc cgggtctata gaagaaaaat tgatcaactt tatcaatgcc gctcaagtta
      901 gcattcatat tgcttcgttt gaattcaact tgccacgcgt agcggatgct ttgatcgccg
      961 ccaaagcccg cggtgtagac gtgcgttggg tgaccgatga tgaaaacggg ttggaaatag
     1021 atggcaatgc gaatcggggg cagttcactc gtttgatggc tgccggagtc gaagtcaagg
     1081 atgatgcggg gcgtactgcc ttgatgcata acaaattctg gctgttcgac cgtcaaatca
     1141 catggacagg ctccacgaat atcaccgtca atggaattta caagcaaaac aataatgtga
     1201 ttgtgatcca ctcgcccgaa gtgacggaga tttacgagcg cgaatgggaa gaattgtgga
     1261 cggcacaact gggtccgcgg gc
//
LOCUS       DAOREF010000917         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_109044_length_1282_cov_3.204564, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000917 DAOREF010000000
VERSION     DAOREF010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109044_length_1282_cov_3.204564"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKI33_15605"
     CDS             complement(<1..294)
                     /locus_tag="PKI33_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38496.1"
                     /translation="MFTVSIPRLRVRSIFLMPLFTIHAMRTSMQAQKAEGILGVDTRF
                     EKNNVVWTKTVWKEEAAMKKYRGSGAHQIAMRILSEMCSEASVARWQQDGTELP"
     gene            complement(297..989)
                     /locus_tag="PKI33_15610"
     CDS             complement(297..989)
                     /locus_tag="PKI33_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNM38497.1"
                     /translation="MVRPSKKTQIPNLQEAIKETAWKQIVDTGAAALSLRAIARGLNI
                     TAPAIYNYFPDRDALVTALIIDAYTSFGDYQIVSRDAQAEGDLVGRLKATGFAYRQWA
                     LEYPQRYQLIFGTPIPGYKAPMMEVLPSAARSLSAPVSVIDELRIANKLNAEGFPSIQ
                     PGFEQMFDVWRSFAGNYDIFSLSVAMIIWSRVHGLVSLEISNNMPPFGVDGTSLYRYE
                     MESIIRQFVKGD"
BASE COUNT          332 a          333 c          287 g          330 t
ORIGIN      
        1 cggcaattct gtcccatcct gttgccaacg ggcaaccgag gcttcgctgc acatctcaga
       61 caaaatccgc atggcgatct gatgcgcgcc cgaaccacga tattttttca tggcggcttc
      121 ttccttccag acggtctttg tccacaccac gttattcttc tcgaagcgcg tgtccacgcc
      181 gaggatgcct tccgcttttt gggcttgcat actcgtgcgc atggcatgaa tggtaaaaag
      241 cggcatgagg aaaatggatc gaactcgcag gcggggaatg gatacggtaa acataactaa
      301 tctcctttta caaattgacg aataatggat tccatttcat aacgatagag agaagtacca
      361 tccacaccaa atggcggcat gttgttggaa atttccaatg agacaagtcc atgaacgcga
      421 ctccagatga tcatggcaac cgatagcgaa aagatatcgt aattccccgc gaaacttcgc
      481 caaacatcga acatctgctc aaagccaggt tgaatggacg ggaatccttc agcatttaac
      541 ttattggcta tccgcagttc atcaataaca ctgaccggcg cacttaatga ccttgcagca
      601 gaaggcaata cttccatcat tggcgcttta taaccgggaa tgggtgtacc aaagatcaac
      661 tgataacgct gcggatattc caatgcccat tgtcggtagg caaaacctgt tgctttcaag
      721 cgccccacca ggtcaccctc tgcttgggcg tcgcgtgaga cgatctggta atccccaaag
      781 gaagtgtagg catcgatgat caaagccgtg accagcgcat cgcgatctgg gaagtaattg
      841 tagatggctg gtgcggtgat gttaagtcca cgcgcgatcg cccgcagcga taatgccgcc
      901 gctcctgtat ccacaatctg tttccatgcc gtttctttga tggcttcctg taaattagga
      961 atttgggttt ttttgctcgg tctgaccatg tcgactcctc ttttaattta cagtgtaaat
     1021 atacagtgtt tattttattt ggtcaagggt aaaacagacc tcggaggttt tgagaagccc
     1081 tgaaaggggg gaacctccga ggtctgctat acaatctttt tcaatgccac ccattccccg
     1141 ctcaatttgg gatgctctgc caaaaccaat ttaccaacat ctactaattt accaactgac
     1201 cgtttcgcca cctcaacccc ccaaccaaat aatttttgca cagcgcgcat ttgcgctgcg
     1261 ccaaccatat cgaagtagag tt
//
LOCUS       DAOREF010000918         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_109259_length_1281_cov_2.696574, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000918 DAOREF010000000
VERSION     DAOREF010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109259_length_1281_cov_2.696574"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..424)
                     /locus_tag="PKI33_15615"
     CDS             complement(23..424)
                     /locus_tag="PKI33_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNM38498.1"
                     /translation="MEIPRHWRLKKQRYGLVGEVCPHCDHKIFPPRDVCPNCGDEAKD
                     LFTFSGKGEVYSYTTIYEAPAGYESQAPYTVALVKLEEGPMVTAQLTDVDNSEVQIGM
                     PVEMVTRKMRNDGDERGLIVYGYKFRPAVTA"
     gene            622..>1281
                     /locus_tag="PKI33_15620"
     CDS             622..>1281
                     /locus_tag="PKI33_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNM38499.1"
                     /translation="MKILLINQVFVSPNEPGHTRHFEMGKYLKERGHELVIVASDLNY
                     QTGQRTVERTGLYAEQNFEGVRVLRSYIYPAIHRSYFWRIIAFFSFMFSSILTALRVK
                     DVDLVLGTTPPIFQAVSALVVASLRGKPLLLEVRDLWPEFGISMGVLKNPIIIALSRW
                     LENFLYKRANHILVNSPAYRDYMLAKNVPANKVTYIPYGTDVDMFNPSIDGSSIRKEL
                     KL"
BASE COUNT          303 a          342 c          289 g          347 t
ORIGIN      
        1 ttggtgcgtg gttatatggt gtttacgcag taactgccgg tctaaactta tacccgtata
       61 cgatcaaacc gcgctcatcg ccatcattgc gcatcttgcg ggtgaccatt tccacgggca
      121 tgccaatttg gacttcggaa ttgtccacat cggtcaactg agccgtgacc atgggacctt
      181 cttcaagttt gaccaatgcc actgtgtagg gagcctgaga ttcatagcca gctggggctt
      241 cataaatggt tgtgtaggaa tacacttcgc ctttaccact aaaagtaaat aaatctttgg
      301 cttcgtcgcc gcagttcggg cagacatcgc gcggggggaa gatcttgtga tcgcagtgag
      361 ggcagacctc accgacgaga ccatatcgtt gttttttgag acgccagtgt cgagggattt
      421 ccatgtgagc aattcctttc gttgactgta attctacgca gtccaactct caaagactct
      481 cagagttact tggctgcgcg tagccagatt tgcaataacg atagcttgca attcaacctt
      541 gcgagaagaa aacagaccaa gcaagcccac tatttactaa tcactattct ctatcttccc
      601 aatgctataa tccctcctcg catgaagata ttacttatca accaagtctt tgtctcgccc
      661 aatgagcccg ggcacacccg tcattttgaa atggggaagt acctcaaaga gcgcggacat
      721 gaactcgtca tcgtcgccag tgacttgaac tatcaaaccg gtcaacgcac cgttgagcgg
      781 acaggtctct acgccgagca aaacttcgaa ggcgtgcgcg tattacgttc ctacatttac
      841 cctgccattc atcgcagtta cttttggcgc atcattgcct tctttagttt tatgttctct
      901 tcgatcttga ccgcgttacg cgtcaaagat gtggacctgg tgctgggtac cacgcccccg
      961 atctttcagg cggtttcagc gttagtggtc gcttcgttgc gtggcaaacc cttgctgctc
     1021 gaagtgcgtg acctgtggcc tgaattcggc atcagcatgg gtgtgctaaa aaatcccatc
     1081 atcattgcgc tctcgcgctg gctcgagaac tttctctaca aacgtgccaa tcatattttg
     1141 gtcaactcgc cagcgtatcg tgactatatg ctggctaaaa atgtacctgc taataaagtc
     1201 acctacatcc cttacggcac cgatgtggat atgttcaatc cctccatcga cggctcgtcc
     1261 attcgcaaag aactcaaact c
//
LOCUS       DAOREF010000919         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_110252_length_1275_cov_3.546721, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000919 DAOREF010000000
VERSION     DAOREF010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110252_length_1275_cov_3.546721"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKI33_15625"
     CDS             <1..589
                     /locus_tag="PKI33_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38500.1"
                     /translation="MVSMTGSLAVLNLSKDADMDYMFVTQPSRLWLARSFAVTFGRIM
                     RLFGDRICVNLLVAESALEWQPQDLYSAREMCQMIPITGMDVYQRLRGVNSWIDTYLP
                     NAKSVPENTPKIHSNQSWIQKLLELPLRGKLGDAIEKWAMDFQIKKITNTYGVGQEAK
                     FSTELCQGNFDNHHFWTNQSFQERLAFLEENALHD"
     gene            582..>1275
                     /locus_tag="PKI33_15630"
     CDS             582..>1275
                     /locus_tag="PKI33_15630"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNM38501.1"
                     /translation="MTKVLFGQSYYLRFDPKLWRAMQPYPPLGTLYAASYIREKGYTV
                     ALFDAMLAESEQEWAQALEKHTPQYAVIYEDNFNYLSKMCLSRMREAAFEMIRMAKER
                     GCTVILCGADVTDHYAKYLEQGADYCILGEGEETLAELLDKLSAGEDARDVIGLASRF
                     TLHASRRPDIKNIDALPFPAWDLVDIEKYKKLWLERHGYFSMNMVTTRGCPYHCNWCA
                     KPIWGQRYNSRSP"
BASE COUNT          336 a          282 c          302 g          355 t
ORIGIN      
        1 aatggtatct atgactggct ctctcgccgt tcttaacctc tccaaagatg cagatatgga
       61 ctacatgttc gtcacacaac caagtcgctt gtggcttgcc cgctccttcg ctgtgacttt
      121 tggtcgcatc atgcgtctct ttggcgaccg gatctgtgtc aacttgcttg tagccgaatc
      181 tgcattagag tggcaacctc aagatttata ctccgctcgt gaaatgtgcc agatgattcc
      241 tatcacaggc atggatgttt atcagcgctt gaggggagta aactcatgga tagacactta
      301 tttgccaaat gcaaagtctg taccagagaa tacgccaaag attcactcga atcaatcttg
      361 gattcaaaag ttgcttgaac ttccactgcg tggaaaactt ggtgacgcca ttgaaaaatg
      421 ggctatggat tttcagatca agaaaattac taatacctat ggcgttgggc aggaagcaaa
      481 attctctaca gagctttgcc aaggcaattt tgataaccat catttctgga ccaatcaatc
      541 atttcaagaa cgccttgctt ttttagagga gaatgccctt catgactaaa gtcctttttg
      601 gtcaatctta ttatctgcgt tttgacccaa aactttggcg tgctatgcag ccatatccgc
      661 cattgggtac attatatgcg gcaagttaca tccgtgaaaa aggatatacg gttgctttgt
      721 ttgatgccat gttggcagag tctgagcagg agtgggcaca ggctttggag aaacatacac
      781 cgcaatatgc tgtgatctat gaagataact ttaattatct ttccaagatg tgtctctcac
      841 ggatgcgcga agctgcgttt gagatgatcc gtatggcaaa ggaacgcgga tgcacagtca
      901 tcttgtgcgg agcggatgtc accgatcatt atgccaaata tctcgaacaa ggcgcggatt
      961 actgcatcct tggtgaaggc gaagagactt tagcagaatt gctggacaag ttatcggcgg
     1021 gggaagatgc gcgggatgtc attggtctag cctcacgttt tacgcttcac gcttcaagac
     1081 gtcccgacat taaaaacatc gatgccctac cgttccccgc ttgggacttg gtagatatag
     1141 agaaatataa aaagctttgg cttgagcggc atggatattt ttcgatgaat atggtcacaa
     1201 cacgtggatg tccatatcac tgcaattggt gtgcgaagcc gatctgggga cagcgctaca
     1261 actcacgttc acccg
//
LOCUS       DAOREF010000920         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_110428_length_1274_cov_4.707137, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000920 DAOREF010000000
VERSION     DAOREF010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110428_length_1274_cov_4.707137"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..953
                     /locus_tag="PKI33_15635"
     CDS             354..953
                     /locus_tag="PKI33_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38502.1"
                     /translation="METIRGKTSLTMDSTELHARLANYKRISLDLGTGDGKFAFCHAQ
                     NFPSHFFIGMDSCRENLREYSRADLPNLLYVIANAQSLPHELHGQISHITINFPWGSL
                     LSSLLNGDSDFLCGLESALGSGAKLEVRLNGGALSEQGWSLEDGSERIFQNLEMAGWR
                     LDAPANMDTHALRNFPSTWAKRLAFGRDPRAIHISASLK"
     gene            complement(979..>1274)
                     /locus_tag="PKI33_15640"
     CDS             complement(979..>1274)
                     /locus_tag="PKI33_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38503.1"
                     /translation="QPLGTSSPLSPQKWADPDWGNEVFLCDYNHFTIPKIFAMLEKTL
                     GNSPFAILETEFDSLYGFITQFEDGIYTSHGWLNFRDKNVYNEFQISNFKIES"
BASE COUNT          344 a          329 c          271 g          330 t
ORIGIN      
        1 atcgaaccta catctaagcc ttaggagtgc cttgttctat ccattgaact atcagagcga
       61 taaaggcatt atacccaaaa aatttcagcc agcactcata ggaaaaatta atacacgagc
      121 ttttatatgc atgctatgat gaaatctatg aacaccacac gccctccctg ctaatcattg
      181 ttctgcgcca cactggcgct ccttgcattt cccctaacgc gtaaaaaacc tacccctgat
      241 gatctagacg gtgcctgacg aagcaaccgt ctatttgcgt ttaagcgcgc tcgtgcaccg
      301 ccccgctcca atttgtctcc ctcaatctca actcgacaaa ttggaggttt cacatggaaa
      361 caattcgggg caaaacgtcc cttactatgg attcgactga actgcatgca cggctcgcaa
      421 attacaaacg catctctctg gaccttggaa cgggtgacgg aaaatttgcc ttttgccacg
      481 ctcaaaactt tcccagccat ttcttcattg gcatggactc gtgccgcgag aacctgcgag
      541 aatattcgcg cgccgatcta cccaatctgc tttacgtgat cgccaacgcc caatccctgc
      601 cgcatgaact gcatggacag atttcacata tcaccatcaa ctttccgtgg ggtagtttgc
      661 tgagcagcct gctcaatggc gactcagact ttctctgcgg acttgaatcc gctttgggtt
      721 cgggcgccaa gctcgaggtc cgcttgaacg gaggcgcgtt atccgaacaa ggctggtcgt
      781 tggaagatgg gtcagagcgc atcttccaaa atcttgaaat ggcaggctgg cgtctcgatg
      841 cccctgccaa catggataca catgcgttac gtaactttcc gagcacatgg gcaaaacgcc
      901 ttgcctttgg acgagatccg cgagcaattc atatcagtgc aagtttgaag taaaaaatag
      961 gtcacgccaa agcgtgacct atgactctat tttgaaattg ctaatctgaa attcgttata
     1021 aacatttttg tctctaaaat taagccatcc atggctagtg tatattccgt cttcaaactg
     1081 tgtaataaac ccatacagcg aatcgaattc agtttcaaga atagcaaacg gggaattccc
     1141 taaagtcttc tcaagcattg caaatatttt agggatagtg aaatggttat agtcgcaaag
     1201 aaagacctca ttcccccagt ctgggtctgc ccatttttga ggtgagagtg gagagcttgt
     1261 acccaaaggt tgaa
//
LOCUS       DAOREF010000921         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_110805_length_1272_cov_4.331142, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000921 DAOREF010000000
VERSION     DAOREF010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110805_length_1272_cov_4.331142"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKI33_15645"
     CDS             complement(<1..739)
                     /locus_tag="PKI33_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNM38504.1"
                     /translation="MRKQTLTWLSLFLVLAVSITACGTASPEATVAPASDATDVAATE
                     TAPTEEPLSGTLSISGAFALYPMMTVWAEEFTKLHPDVQFDVQGGGAGKGMTDTIAGA
                     VDIGMISRSIKPEEEAQNIFWVSVTKDAVFPIINAENPVAQQILEKGISQEIFAKIYI
                     TGEITTWGEVVGDPSITDKINVFTRSDASGAAEQWAKYAGGKAQEDLKGIGVNGEPAM
                     LDTILKDPLGVGFGNLNSIFDLSGGGLV"
     gene            1010..>1272
                     /locus_tag="PKI33_15650"
     CDS             1010..>1272
                     /locus_tag="PKI33_15650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNM38505.1"
                     /translation="MKLTTRSEYALLALVYLARNEDEGFISIDTIATAQGIPPKFLEQ
                     LMLALKRAHFLRSAKGQKGGYQLAKRPDQISLAEVIRLFDGALA"
BASE COUNT          300 a          307 c          306 g          359 t
ORIGIN      
        1 gcacaagtcc gccgccgctc agatcgaaaa tgctgttgag gttgccgaag ccaacgccaa
       61 gcgggtcttt caaaatggta tcgagcatgg ctggctcacc gttgacacca atccccttca
      121 ggtcttcttg tgcttttcca cctgcatact ttgcccactg ttcggctgca ccggaggcgt
      181 cggagcgagt gaagacattg atcttgtccg tgatggatgg gtcgccgacc acttcgcccc
      241 aggtggtaat ttcgccggtg atgtaaatct tggcaaagat ttcttgcgaa ataccttttt
      301 caagaatctg ttgagcaaca ggattttctg cattgatgat cgggaagacc gcatccttcg
      361 tcacggacac ccagaagatg ttctgagctt cttcttccgg tttgatactg cgcgagatca
      421 tgccaatgtc cacggcacct gcaatggtgt cggtcatgcc tttgcccgcg ccgccgccct
      481 gcacatcgaa ctgaacgtca gggtgtaact tggtgaactc ttctgcccaa acagtcatca
      541 tggggtagag ggcgaatgcg ccagagatgg acagtgtgcc gctgagaggt tcttcggtgg
      601 gggcggtttc tgtggcagcg acatcagtcg catcagacgc aggggcgact gtggcttccg
      661 gtgaggcagt tccgcacgct gtgatggata ctgccaaaac caagaatagc gacagccatg
      721 ttaaagtttg ttttcgcata ttgtttctcc aattacgttt gtttttatat aaagcctatt
      781 gatttagtag gaatttaagt taaataaaaa gacttctttt atttcaagac ctcgagtatc
      841 ttgataaatc ctactagact aataggttat gtgtacttat cataattcag cgactgaatt
      901 ttgtcaatac tgaaagatgt ttgaaccgac cattttgcct cgacttgaca tgcttatttt
      961 ttatactata ctgctcccgt aggtcaaatc agaaaagttg agttattaga tgaagctaac
     1021 cactcgtagt gaatatgccc tccttgccct tgtttatctt gcccgtaatg aagatgaagg
     1081 gtttatttcc attgatacca tagccacagc ccaagggatt ccgcctaagt tcttagagca
     1141 gctcatgcta gcactcaagc gtgcgcattt cttgcgcagc gccaagggac aaaaaggcgg
     1201 ctatcagctt gccaaacgtc ccgatcagat ttccctcgcg gaagttattc gtctttttga
     1261 tggtgccctt gc
//
LOCUS       DAOREF010000922         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_111398_length_1269_cov_2.589786, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000922 DAOREF010000000
VERSION     DAOREF010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111398_length_1269_cov_2.589786"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKI33_15655"
     CDS             <1..661
                     /locus_tag="PKI33_15655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM38506.1"
                     /translation="EILESKYEAQNVLLAPHGSFEEPTNTEPSTMPGGRPTVMTFGKF
                     GTYKTVDLLVEAHAELLKNRPDMRLVIAGTDSPNAAGYLQGVREKYAHVPNVEYTGYV
                     TEEDVPRIFGEAWVVAFPYTSTTGSSGVLHQAGGYGKAVVLPNLGDFAELVLEEGYDA
                     EFFEPTSAESLAESIAKVIDYPERQKEIGLRNYTAANGLPITEVVDWYLIHFEELLKK
                     K"
     gene            676..1008
                     /locus_tag="PKI33_15660"
     CDS             676..1008
                     /locus_tag="PKI33_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNM38507.1"
                     /translation="MELIKLSLNTLPVLKLKGRFDAHEVEPVNTWLAEQTKAGNVKII
                     VNLEGVNFIDSTALSTLVRGLKLCREKNGDLHVCSLQQPVRVIFELTRLDKAFDIFAT
                     EEEAAKGF"
     gene            1089..>1269
                     /locus_tag="PKI33_15665"
     CDS             1089..>1269
                     /locus_tag="PKI33_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38508.1"
                     /translation="MQLRKGDEVLIGCPDSMSGREPSLEVTSLSSGLSLHIFGVTDMR
                     WVPTAESMLDIFSSLL"
BASE COUNT          319 a          261 c          351 g          338 t
ORIGIN      
        1 ggagattttg gaaagcaaat atgaggcgca gaatgtattg cttgcgcctc atggttcttt
       61 tgaagaaccg acgaatactg aacctagcac catgccgggc ggtcgcccaa cggtaatgac
      121 ctttggaaag tttggcactt ataaaaccgt tgacttgttg gtggaagcac atgccgagct
      181 gttgaagaat cgtccggata tgcgcctggt tattgccggg acggatagcc ccaatgccgc
      241 tgggtattta caaggagtgc gtgaaaaata tgctcatgtg cccaatgtgg aatacacagg
      301 ctatgtgacg gaagaggatg ttccgcgcat ttttggcgaa gcatgggtgg tggcatttcc
      361 atacacatcg acaacgggta gttcgggtgt gttgcatcag gcaggcggtt atggcaaggc
      421 agtggtctta ccaaatttag gtgacttcgc cgagctggtt ttggaagaag gctatgatgc
      481 tgagtttttt gagccaacca gtgcagagag tttggcggag tcgattgcga aggtgattga
      541 ttaccctgaa cgacagaaag agatcggctt acgcaattac accgccgcaa atggcttgcc
      601 cattacagaa gtggtggatt ggtatttgat tcattttgaa gaattattga agaaaaagta
      661 aagccaagga gacatatgga acttatcaag ttatccctta ataccctgcc tgttttgaag
      721 ctcaaaggtc gctttgatgc gcacgaggtg gaacctgtca atacctggtt ggcagagcaa
      781 accaaggctg gcaatgtaaa gatcattgtt aatttggaag gcgtgaattt cattgactca
      841 accgcgcttt caaccttggt gcgcggactc aaactttgcc gcgaaaagaa cggtgatttg
      901 catgtctgtt cgttgcaaca gccagtgcgc gtcatttttg aattgacccg gttagacaaa
      961 gcctttgata tcttcgccac tgaggaagaa gcagcgaaag gtttctagtg ctccctgaga
     1021 tcactactaa acaccgtgag agtttcaaat ccctggcatc tggttggatg cgagtcggtg
     1081 caacttgtgt gcaactgcga aagggagatg aagttctgat tggctgccca gattcaatgt
     1141 ctggtcgtga gccgtcgctt gaggtgacat cgctctcttc agggttatcc ctgcatattt
     1201 ttggggttac cgatatgcga tgggtgccaa cggcggaatc catgctggat attttttcca
     1261 gcctgctta
//
LOCUS       DAOREF010000923         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_111550_length_1268_cov_3.188788, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000923 DAOREF010000000
VERSION     DAOREF010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111550_length_1268_cov_3.188788"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKI33_15670"
     CDS             <1..554
                     /locus_tag="PKI33_15670"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNM38509.1"
                     /translation="NLSFPVYSLVGGKWTSFRAFSEEVADKSLQFLGIKRNKGTQELP
                     VGGGRNYPRTSEERTKYINGLAAWTGLSHERLETLFERYGTRAESIAEFMKVATDAPL
                     NSLPSYSKRELLFLIQHEKAEHLDDLLLRRTMLAMLGKLTRDAVIECSMLMADALNWN
                     LERRNAEAERTLRVLADRHRVRL"
     gene            618..>1268
                     /locus_tag="PKI33_15675"
     CDS             618..>1268
                     /locus_tag="PKI33_15675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM38510.1"
                     /translation="MSPRQKSKLQIDLVIPVYNEAEAIRQTYADIRKVVDALPYDFRF
                     IYVDDGSSDGTADTLRGISTDDPRVTLLQLSRNFGHQAALTAGMDASQGDVMISMDGD
                     GQHPPALIPKMVSLIEQGYDVVQGQRLEEERAASFKKVTSNFFYWLINRISGTQVLQG
                     AADFRALSRNALNGIRSMHEYHRFLRGMISWMGYNSVILPYHEPDRIAGKSKYSLGK"
BASE COUNT          297 a          321 c          335 g          315 t
ORIGIN      
        1 ccaacctaag cttcccggtc tattctctgg tgggtggcaa gtggacctcc ttccgtgctt
       61 tctctgagga agtggcagat aagtctttgc agttcctcgg tattaagcgc aataaaggca
      121 cacaggaact tccagttggc ggcgggcgca attatccgcg cacgagcgaa gagcgcacca
      181 aatacatcaa cggtctcgcc gcgtggacgg ggctttcgca tgaacgcctt gaaacccttt
      241 tcgaacgtta cggcacgcgc gccgaatcta ttgctgagtt tatgaaagtg gcaactgatg
      301 cccctcttaa ttcgttgccg agttattcta aacgcgaatt gctcttcttg attcagcatg
      361 aaaaggcaga gcatcttgat gatctacttc tgcgtcgcac catgcttgcc atgttgggca
      421 aactcacacg cgatgcagtg atcgaatgct ccatgttgat ggcagatgcc ctgaattgga
      481 acctcgaacg tagaaatgct gaggcggaga gaacgctgcg tgtcttggca gaccggcacc
      541 gggtccggtt atgaagcgat ataaacgaac gccgccgtta tcggcggtgt ttttgtctgc
      601 gtggtagaat cttcctgatg tctccacgtc aaaaatccaa actccagatc gaccttgtca
      661 ttcctgttta taacgaagct gaagccattc ggcaaaccta tgcggacatt cgcaaagtgg
      721 tggatgcgct gccgtacgat tttcgattca tctatgtgga tgacggctca agcgacggga
      781 cagcggatac gcttcgaggg atctcaactg acgacccaag agtgactctg ctccagctca
      841 gccgcaactt tggtcaccaa gccgcgttga ccgccggtat ggacgcctcg caaggcgacg
      901 tgatgatctc gatggatggg gatggacaac acccgcctgc tttgattccc aagatggtca
      961 gtttgatcga acagggctac gatgtggttc aagggcagcg tctcgaagaa gaacgtgcgg
     1021 cttcgttcaa gaaagtcact tctaactttt tctattggct tatcaaccgc atcagcggta
     1081 cacaggtttt acaaggggct gcagattttc gcgccttgtc gcgtaatgca ttgaacggca
     1141 ttcgttcgat gcacgaatat catcgtttct tgcgcggcat gatttcatgg atggggtaca
     1201 acagcgtcat cctgccgtat catgagccag accgtattgc tgggaaatca aagtattcac
     1261 ttggcaaa
//
LOCUS       DAOREF010000924         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_112664_length_1262_cov_2.217067, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000924 DAOREF010000000
VERSION     DAOREF010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112664_length_1262_cov_2.217067"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKI33_15680"
     CDS             complement(<1..544)
                     /locus_tag="PKI33_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNM38511.1"
                     /translation="MSRKKNAMAYGFTALLVIQLACSSLSPSADVPTEMTVPLGPQVS
                     DTEQQTKVLESIFSQIDTNYIYAENSSIDLKALRTTYQSEIDKGLTSSEFNELMNRFA
                     AEFPAGDIVYATREERIKNDTAASLVGYGGIGAFVNFQAKDKPHVVILDVIPGSPAEK
                     AGLKAHDSIYAVDGDPVLLEE"
     gene            complement(601..822)
                     /locus_tag="PKI33_15685"
     CDS             complement(601..822)
                     /locus_tag="PKI33_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38512.1"
                     /translation="MTETTIRVGDQVKIRLDEKFGEREGWYTGKVVRIDPYSEHRSFY
                     WVRLDAEAQAILRVAEISVFNPKNIKKIA"
     gene            complement(822..1007)
                     /locus_tag="PKI33_15690"
     CDS             complement(822..1007)
                     /locus_tag="PKI33_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38513.1"
                     /translation="MLNTIIKILAVLLIMTGIIWILQGVNILPGSFMTGDPQWAINGA
                     ITAVIGAGLFWFSSRKK"
BASE COUNT          315 a          312 c          275 g          360 t
ORIGIN      
        1 cttcttccaa aagcacgggg tcaccatcta cagcatagat gctatcgtgt gctttcaagc
       61 ctgccttctc cgcaggtgac ccgggaatga catctaagat caccacatgc ggtttgtcct
      121 tcgcttgaaa gttgacaaag gcgccaatgc cgccgtatcc tacaaggctg gcagcggtgt
      181 catttttaat tctttcttca cgggtggcgt aaacaatatc accagcaggg aactctgctg
      241 cgaatcgatt catcaattcg ttgaattctg aagaggttag ccctttatca atttctgact
      301 gatacgttgt gcgcaatgct tttaagtcta tgctgctgtt ttctgcataa atgtaatttg
      361 tatcgatctg agaaaatatg ctctcaagaa cttttgtctg ctgttccgtg tctgatacct
      421 gcggacccaa tggcacggtc atttctgtgg gtacatctgc actgggagac aagctggaac
      481 atgccaattg gatcacgagt aatgctgtaa acccatacgc cattgcattc ttttttctac
      541 tcatctaaaa ctccttccgc atgattaaga tggcgataca gatgccccct cttacgaaag
      601 ttaagcaatc tttttgatat tctttgggtt gaacaccgaa atctcggcaa ctcttaatat
      661 agcttgcgct tcagcatcca accgcaccca ataaaagctg cgatgttcag aatacgggtc
      721 aatgcgaacg accttgccgg tgtaccagcc ttctcgttcg ccaaactttt cgtcaaggcg
      781 aattttaact tggtcaccga cacgaatggt tgtttcagtc attacttttt gcgggagcta
      841 aaccagaaca agccagctcc aataaccgct gtgattgcgc cgttgatcgc ccattgtggg
      901 tctccggtca taaaactacc tggcaagata ttgacgcctt gtagaatcca aatgataccg
      961 gtcatgatca agagaacagc aagaattttg ataatagtgt ttagcatgat tttttcctga
     1021 attgatttat ttataaaact ccctgcccaa gcggacaggg agttgacttg atacatttcg
     1081 cttcgttctg gacctgttac ggattcggcg ttccttgaac tgcgacacca acccaggtga
     1141 agacacgtct ttcgctggtt gcaccagagg agatccagat tggcttgcct tggtcgtcca
     1201 ctccagattg gaggacgggc acgacccacc aacgcatgac atgtgccaca ttatccttcg
     1261 gg
//
LOCUS       DAOREF010000925         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_114037_length_1254_cov_4.201001, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000925 DAOREF010000000
VERSION     DAOREF010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114037_length_1254_cov_4.201001"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>1254)
                     /gene="dnaA"
                     /locus_tag="PKI33_15695"
     CDS             complement(102..>1254)
                     /gene="dnaA"
                     /locus_tag="PKI33_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNM38514.1"
                     /translation="DTLNSKVSVKFIVSQSEDNASIADLETAPASIEITPPEPKPRHV
                     TLNPRYTFDTYVVGSGNRLAHAACQAVAEKPARAYNPLFLYGGVGLGKTHLLHAIGNA
                     CHASGLNVLYVSSEEFTNDMINAIRSHTTQAFREKYRSADVLLIDDIQFIAGKESTQE
                     EFFHTFNTLHGQDKQIIVSSDRPPKSLVTLEERLRSRFEWGLTADIQAPDLETRLAIL
                     RSKAERTGRNISDEILESIAQRVQSNIRELEGALNRIIAYADLSGASLTPNLVEVALA
                     DLMPSRADIEPAHVVDLVARKFGLTSEKLLGRDRTKEVALPRQIAMYLLREEAKISFP
                     QIGEVLGGRDHSTVMSAYEKIKEQIHADRRLEQDIVSIKQQLYNQSVNA"
BASE COUNT          313 a          268 c          345 g          328 t
ORIGIN      
        1 tgaagtaaat cgtataaaag cgattaatgc attggttaac cataaaataa aaacaagcgc
       61 acctcattag gtgcgcctgt tcatttcggt tcctgtattt attatgcatt tacgctttgg
      121 ttatataact gctgtttgat agaaacaata tcttgttcca aacggcggtc ggcgtgaatt
      181 tgttctttga ttttttcata agcagacatg acagtcgagt ggtcgcgtcc gccgaggact
      241 tcgccgatct gcgggaaaga gatcttagct tcttcacgca agagatacat agcgatttga
      301 cgaggtaatg ccacttcttt ggtgcggtcg cgcccgagta atttttcaga ggtgagacca
      361 aatttgcgag caaccaagtc cactacgtgt gcaggttcaa tgtctgcacg ggagggcatg
      421 aggtcggcaa gggcaacttc gacgagattg ggagtaaggg atgctccgct caggtctgcg
      481 taggcaatga tgcgattgag agccccttca agctcacgga tattcgattg cacgcgttga
      541 gcaatgcttt cgagaatttc atcagagata ttacgcccgg ttcgctcggc tttagagcgc
      601 aaaatagcga ggcgggtttc aaggtctggc gcttgaatat cggcagttaa gccccactca
      661 aagcgggagc gcaagcgttc ttccagcgtc acaagggatt tgggcgggcg atcagacgaa
      721 acgatgatct gtttgtcttg tccgtgcagg gtgttgaagg tgtggaagaa ctcttcctga
      781 gtggattctt tcccggcgat aaattgaata tcatcgatca acaacacatc agccgaacgg
      841 tatttttcac gaaaggcttg cgtggtatgt gaacgaatgg cattgatcat gtcattcgtg
      901 aattcttcag aagaaacata gaggacgttg agtccgctgg catgacaggc attaccaatg
      961 gcatgtaaga gatgggtctt gccgagtccc acgcctccat acaggaagag cgggttgtat
     1021 gcacgtgctg gtttctcagc cactgcctga caagccgcat gtgcgaggcg gtttcccgaa
     1081 ccgaccacat aggtatcgaa tgtatagcgc ggattgagcg ttacatggcg gggttttggt
     1141 tccggcgggg taatctctat cgaagcagga gcagtttcaa ggtccgcaat agaggcgtta
     1201 tcttccgatt gggaaacaat aaatttcaca gagacttttg agttcagcgt gtcg
//
LOCUS       DAOREF010000926         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_115654_length_1245_cov_3.571429, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000926 DAOREF010000000
VERSION     DAOREF010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115654_length_1245_cov_3.571429"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKI33_15700"
     CDS             <1..390
                     /locus_tag="PKI33_15700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNM38515.1"
                     /translation="KSIRKAYEMGVPIAMGTDAATPYNFHGENAMELYYMSQAGISAM
                     DCIVASTKNAARALGWDSKLGTLKAGKLADLIVVKKNPLDDLRSLANKNNIEYVMQGG
                     KFVARQFVEPTGIPEELMAGAWICCGF"
     gene            409..>1245
                     /locus_tag="PKI33_15705"
     CDS             409..>1245
                     /locus_tag="PKI33_15705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNM38516.1"
                     /translation="MKRVYQYLSVYVFLALACSSLTPSTSTGTGYRYDNHPDANTQSA
                     EYRALSKWGKTDITYYFINGTDKISGDREQELVAQAFALWAAQTSLTFTETNSRQSAD
                     IVIGWAEGEHGDGDPFDGPGDVLAHASYPNPYEERQVFLHFDDAERWVDSDTQDVDLL
                     TVAAHEIGHALGLGHSNDPNSLMYPSYSEPHRFLGGDDIAGIQSLYGLASEPPTAPEV
                     PKPNENPPTSPSQDSDGDGLADSDETLITGTDPNNKDSDSDGIGDGVEVVNRMNPLDA
                     DMD"
BASE COUNT          341 a          296 c          311 g          297 t
ORIGIN      
        1 aagtctattc gtaaggcgta tgaaatgggt gtacccattg ccatgggaac agatgccgct
       61 acaccgtata actttcatgg cgagaatgcg atggagctat attacatgtc gcaggcggga
      121 atctctgcaa tggactgtat tgttgcttct acgaagaatg ccgctcgcgc actcggctgg
      181 gactcaaagc ttggcacgct gaaagccggt aaattagccg atctgatcgt ggtcaaaaag
      241 aatccgttag atgacctgcg ttctttagcc aataaaaata acattgaata tgtcatgcaa
      301 ggcggtaaat ttgtggcgcg ccaattcgta gaaccgaccg gcattcctga agaattgatg
      361 gctggcgctt ggatctgctg cggattttag ttttattgga ggttaaaaat gaaacgcgtt
      421 tatcaatatc tgtctgtata tgtttttctc gcacttgcct gttccagcct tacaccgagt
      481 acatccactg gaacaggtta tcgatacgat aatcatccgg atgccaatac acagtcggct
      541 gaatatcgcg cactttccaa atggggcaaa accgatatca cctattattt catcaacggc
      601 acagacaaaa tttcaggaga ccgagaacag gaattggtgg ctcaggcatt tgcactatgg
      661 gcagcgcaaa cttcactcac tttcacagag accaactcaa gacaaagcgc ggatattgtc
      721 attggctggg cagaaggtga acacggagat ggcgacccat ttgacggacc tggcgatgta
      781 ctagcacacg cctcttaccc caacccgtat gaggagcgcc aagtcttttt acattttgac
      841 gacgccgaac gctgggtgga tagcgatact caagatgtag acctgcttac cgtagcagca
      901 catgagatcg gtcatgcgtt gggactgggt cacagcaacg acccgaactc tctgatgtat
      961 ccatcctata gtgagccgca tcgttttcta ggcggggatg atattgcggg cattcaatca
     1021 ctttatgggt tggcaagtga accacctacc gcgccagaag tgcccaagcc aaatgaaaac
     1081 ccgcccacca gcccaagcca ggactctgat ggagatgggc tggcagactc agacgaaact
     1141 ctcatcacag gtacagaccc caataataaa gattcagata gtgatggcat tggcgatggc
     1201 gtagaagtgg tcaaccgcat gaatccgctt gatgctgata tggat
//
LOCUS       DAOREF010000927         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_116219_length_1242_cov_3.841618, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000927 DAOREF010000000
VERSION     DAOREF010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116219_length_1242_cov_3.841618"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKI33_15710"
     CDS             complement(<1..788)
                     /locus_tag="PKI33_15710"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNM38517.1"
                     /translation="MLAKMQSYFKRQIAFPQDHGSWVFIFSPLMVGLFAGKTFQAATI
                     YLITAALSAFLIRQPITILVKAISGRRSAADLPAARFWLLVYGIIAAVSLIGLILSGY
                     SYLIYLAVPGAPVFAWHLWLVSKRAERKQLGVEVLATGVLALVAPAAFWVGIGKYNPH
                     GWWLWLFTWLQSAASIVYAYLRLSQRDMSKEQAQATSRAAWWKMGSRALLYTSFNMLL
                     SASLGISALVPLFIFVPFLVQWLETLLGITHPSVGWKPIQIGMR"
     gene            894..>1242
                     /locus_tag="PKI33_15715"
     CDS             894..>1242
                     /locus_tag="PKI33_15715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNM38518.1"
                     /translation="MAKKKLAKKNNPQTASSQKTIQYVAGGVILLVVAFFVWQYFPKS
                     APKQDAAICEQFADIPVADQYDSSPPMKVDAAKKYFATVEMENGGQFKMELYPDKAPI
                     TVNSFVFLSCKGFY"
BASE COUNT          367 a          280 c          301 g          294 t
ORIGIN      
        1 tggcgcatac caatttggat aggcttccaa cccacagatg gatgggtgat tcccaaaagc
       61 gtttccagcc attgcaccaa aaatgggacg aagatgaaca gcggcacaag agcggatatc
      121 cccaagcttg ccgagaggag catattgaag gatgtataca gcaatgcgcg gcttcccatc
      181 ttccaccacg cggctcgact cgtcgcctgt gcctgttcct tcgacatatc cctttgactc
      241 agccggagat aagcatacac aatgctggca gctgattgca gccatgtaaa taaccaaagc
      301 caccaaccat gggggttgta tttgccaatg ccaacccaaa aagcagcggg tgcgaccagt
      361 gctaatacac cagttgctag cacttctaca cccagttgtt ttcgttcagc gcgtttactt
      421 accagccaca aatgccatgc aaagacgggt gcccccggca cagccaagta gataagataa
      481 ctgtagccgg agaggataag tccgatcaaa gacacggcgg caataatgcc atagaccagc
      541 aaccagaaac gggcggcggg aagatccgca gcggagcgcc gtccactgat ggctttgacg
      601 agaatcgtga ttggttgtcg aatcaggaat gcagataacg ctgcggtgat gagataaatc
      661 gttgcggctt gaaaggtttt gccagcaaaa agaccgacca ttaacgggct gaaaatgaaa
      721 acccatgagc cgtgatcttg tggaaaagcg atttgccgtt tgaagtacga ttgcattttg
      781 gcaagcatac aggaaaaaga attcgaacgc aaggtgccat ctctcatgtg tttattaagg
      841 aaagaaggtc aaggttgtta taattgagaa aattttttag gagagaaatt cgtatggcaa
      901 aaaagaagtt ggctaagaaa aataatccgc aaaccgcatc ttcacaaaaa acaatccagt
      961 atgttgctgg cggtgtcatt ttgcttgtgg ttgcattttt tgtttggcaa tattttccaa
     1021 agagtgcccc taaacaagat gccgcgattt gtgaacagtt tgctgatatt cctgtagcag
     1081 accagtatga ctcatctccg cccatgaagg tggatgctgc caaaaaatat tttgcaactg
     1141 ttgaaatgga aaatggcggt cagttcaaaa tggaacttta ccccgataaa gcgccgatca
     1201 ctgtcaatag ctttgttttc ttgtcttgca aaggctttta ca
//
LOCUS       DAOREF010000928         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_116226_length_1242_cov_3.228307, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000928 DAOREF010000000
VERSION     DAOREF010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116226_length_1242_cov_3.228307"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKI33_15720"
     CDS             <1..216
                     /locus_tag="PKI33_15720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNM38519.1"
                     /translation="GEELKGDTAVITSALPLSNDEQASVKKSVVAKDYSFKVDPSILG
                     GLVIKVDEKVLDASVAGKLEGLRSALK"
     gene            298..>1242
                     /gene="murE"
                     /locus_tag="PKI33_15725"
     CDS             298..>1242
                     /gene="murE"
                     /locus_tag="PKI33_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HNM38520.1"
                     /translation="MFLKDLFVDFPFSRPSNIPDVEINEIVIDSRAVKPGNLFIAMRG
                     RDADGHTYIQKAIDNGAVAVIGDKELSGFSVPYIQLENARHSLTWLAAAFHGFPARKL
                     TVVGVTGTDGKTTTSNLLYKILIAAGLKAGMISTVNAVIGDEVLDTGFHVTTPDAHDV
                     QRYLAKMVAAGLTHVVLETTSHGWSQHRIDACEFDIAVVTNITHEHMDEHGGYENYRA
                     AKARLFESLEWTRAKPQGNPRLGVINFDDKKSFDFLNDFIKTKKINYGLGEGSDVRGM
                     NIAYSPSGIHFTAVGKDFRVAVSSNLVGAYNVSNCLAAL"
BASE COUNT          324 a          280 c          310 g          328 t
ORIGIN      
        1 ggtgaagaac tcaagggcga taccgccgtg atcaccagcg cgctcccact ttctaatgac
       61 gagcaggctt ctgtgaagaa gagtgtcgtt gccaaggatt actccttcaa ggttgacccc
      121 tccatcctcg gtgggttggt cattaaggtc gacgaaaagg tgctcgatgc ttccgtcgcc
      181 ggtaaacttg aaggtctgcg ctctgcactc aagtaagact aaaacgatat caaaaaggct
      241 ttggtaaaat taccaaagcc ttttttgttt aacttcaatg gagataaact agcaactatg
      301 tttctcaaag acttgttcgt tgactttccc ttttctcgtc cttctaacat ccctgatgtt
      361 gaaataaatg aaatagtcat tgatagccgg gctgtaaagc cgggtaatct atttatcgcc
      421 atgcgcggac gtgatgcaga tgggcatacc tatattcaaa aagcaataga taatggtgct
      481 gttgcggtta ttggggataa agaactgagc ggatttagcg ttccgtacat tcagttggag
      541 aacgcgcgtc attccttgac ctggcttgct gcggcgtttc atggctttcc tgcccgtaag
      601 ctgactgttg tcggtgtgac cggcacggat ggcaaaacga caaccagtaa tctgctttac
      661 aaaatcctca ttgcggctgg actcaaagca ggcatgatct ctacggtgaa tgccgtaatc
      721 ggtgacgagg tgttggatac tggtttccac gtcaccacac cagacgccca cgatgtgcaa
      781 cgttatttgg caaaaatggt tgctgctggc ttgacccatg ttgtgctgga aaccacgtct
      841 catggttggt ctcaacatcg tatcgacgcc tgcgaatttg atattgcggt tgtgaccaac
      901 attacacacg aacatatgga cgaacatggc ggctatgaaa actatcgcgc tgccaaggca
      961 agattatttg aaagcctcga atggacacgc gccaagccgc aagggaatcc gcgtttgggt
     1021 gtgatcaact tcgacgataa aaagtccttt gattttctga acgactttat caagacaaag
     1081 aaaatcaatt atgggctggg cgaagggagc gatgttcgcg ggatgaatat cgcatacagc
     1141 ccatctggaa tccactttac ggcggtaggc aaagattttc gtgtagcagt atcaagtaat
     1201 ttggttgggg catataacgt ctcaaattgc cttgccgcgc ta
//
LOCUS       DAOREF010000929         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_116241_length_1242_cov_2.830666, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000929 DAOREF010000000
VERSION     DAOREF010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116241_length_1242_cov_2.830666"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..724)
                     /locus_tag="PKI33_15730"
     CDS             complement(98..724)
                     /locus_tag="PKI33_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38521.1"
                     /translation="MLIHPSPYERITALTDIFLGILAAWGAAQLFAFADFRHQIWAWT
                     FCLLAIASFLGAIAHGFVMSTKTNDRLWMPINVALGLTLGLFVVGALFDLSGESVARQ
                     ALPVMIALGFGFFLITILKSGTFMTFIAYEALAMLFALGIYGYLFFTSNLLGVGWMVL
                     GVLVTIIAAVVQATGKAGKSIFWYFDNNGVFHLIQMIGMVLLLIGLQQ"
     gene            complement(718..>1242)
                     /locus_tag="PKI33_15735"
     CDS             complement(718..>1242)
                     /locus_tag="PKI33_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNM38522.1"
                     /translation="PQNDTEFEQYIALLTETKAALSPHGLLVSVALPAESSFPLGEFA
                     VVDRVHLMSYDRGERHSTFEQSVADVEDFLEAGIPPEKLILGMPFYGRGMSSPSDTYS
                     YEQIRSTYAPAPDVDEVGDIYFNGLNTIRHKVCYGIAQNLGGFMVWELGLDTVDESSL
                     LQQIYRLAQGAESC"
BASE COUNT          381 a          310 c          271 g          280 t
ORIGIN      
        1 gatgagatcg atgagttgat cgtcgagttc aataaagaag ttgggttatc gtaaacgcat
       61 ccagacctcc gaggtttttg aaacctcgga ggtcttttta ttgttgtagc ccaatgagca
      121 acaaaaccat cccgatcatc tgaatcaagt gaaagacccc gttattatcg aaataccaga
      181 agatactctt gccagctttt ccagtcgctt gcaccacggc agcgatgatc gtcaccaaca
      241 ccccaagcac catccaccct acacccagca aattagatgt aaaaaataaa tagccgtata
      301 tgcccaatgc aaagagcata gcaagcgctt cataggcgat gaaggtcata aaagttccgg
      361 actttaagat agtaatgagg aaaaagccga agcctagcgc gatcatcaca ggcaacgctt
      421 ggcgagccac gctttcacca cttaggtcaa acaatgcccc cacaacaaac aagcctaagg
      481 tcaaccctag tgctacatta attggcatcc ataaccggtc atttgttttg gttgacataa
      541 caaatccgtg ggcgatggca ccgagaaaag aggcgattgc cagcaaacaa aaggtccatg
      601 cccaaatttg atggcggaaa tcggcgaagg caaaaagttg agccgcaccc catgctgcca
      661 gaatccctaa aaagatatcg gtcaatgcag tgatacgttc atacggggaa ggatgaatta
      721 acatgactct gctccttgtg ccaagcgata tatctgttgg agtaatgaac tctcatccac
      781 tgtatctagc cctaactccc aaaccatgaa tccgcccaag ttttgcgcga tgccataaca
      841 aactttgtgc cgaatggtgt tcaagccgtt gaaataaata tcccccactt catccacgtc
      901 tggtgcaggc gcataggtag accgtatctg ttcataagaa taggtatcag atggcgaaga
      961 cattccacgc ccatagaaag gcatgcctaa gatcaacttc tcaggtggaa tacctgcttc
     1021 taaaaaatcc tccacatctg ccacggattg ctcaaaggtg gagtgtcgtt cgccgcggtc
     1081 ataagacata agatgcacac gatcaacaac cgcaaactct ccaagtggga aactcgactc
     1141 agctgggagg gcaacactca ccaacaagcc atgcggggaa agagcagctt tagtttcggt
     1201 taataaggct atgtactgtt caaactctgt gtcgttttgt gg
//
LOCUS       DAOREF010000930         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_116398_length_1241_cov_4.381956, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000930 DAOREF010000000
VERSION     DAOREF010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116398_length_1241_cov_4.381956"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKI33_15740"
     CDS             <1..308
                     /locus_tag="PKI33_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNM38523.1"
                     /translation="GNPTKPFLIANIVYNLLYIGIFFVLITGVWIAYQRNLLGYLLDP
                     NSAFGRGGFKLLADIHKLGWEILLGWILLHIGIMSYRQFLKKETILERMWFTKNNQK"
     gene            402..893
                     /locus_tag="PKI33_15745"
     CDS             402..893
                     /locus_tag="PKI33_15745"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNM38524.1"
                     /translation="MNTPKRYHPILVTLHWLTVIFMLGAGFLSEEGGNSPINFHMILG
                     ALLLIVMVARLITRFSTARPAALDAGNAFFNKIGELTHLGLYAVAFAILGMGGAIAVK
                     RNLFGYLMDNTAQISRAGFLGDVHHLFYLMAMALIFLHVGAALYHQFMLKDNILSRMW
                     YGK"
     gene            976..>1241
                     /locus_tag="PKI33_15750"
     CDS             976..>1241
                     /locus_tag="PKI33_15750"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNM38525.1"
                     /translation="MPSVQLFITCLTDSFFPETGEAVVDILSRLGISVDFTPKQTCCG
                     QPQFNAGLRNDARAIAEHTIKVFEKTSGDIVTPSGSCAHNFRHNL"
BASE COUNT          318 a          288 c          249 g          386 t
ORIGIN      
        1 tagggaatcc tacaaagcca tttcttatcg caaatattgt ctataacctt ttgtatattg
       61 gcatcttttt tgttcttatt acaggagtgt ggatcgccta tcaaagaaat ttactcggct
      121 atttattaga tccaaattct gcatttggaa gaggcggttt caaacttctt gctgatatcc
      181 acaaattagg atgggaaatt ttgctcggtt ggatcctttt gcatattggc ataatgtcct
      241 atcgtcaatt tctcaaaaaa gaaacaattt tagaacgtat gtggtttaca aaaaacaatc
      301 aaaaataatt atggggaaat tatggggaaa ccttcaagaa ttagattttg taatccggct
      361 ctaatcatgc agttctatag tatgggcaag gagagcaaaa aatgaatacc ccaaaacgtt
      421 atcatcccat tctcgttacg ctgcactggc ttactgtgat ctttatgctt ggtgcaggct
      481 tcctttcaga agaaggtggc aattccccga ttaactttca catgattctt ggtgcgcttc
      541 ttttgatcgt tatggttgca cgcttaatca cccgtttttc cacggcgcga cccgccgcat
      601 tggatgcagg caatgccttc ttcaataaga tcggtgagtt gactcatctt ggattgtacg
      661 cagttgcatt cgccattctc ggcatgggtg gagccattgc agtaaaacgc aatctatttg
      721 gttatcttat ggacaatacc gctcaaatca gccgtgctgg ttttcttggt gacgttcatc
      781 acctctttta cctcatggca atggcattga tctttttaca tgtaggcgct gccctctacc
      841 accaatttat gctcaaggac aatatcctct cgcgcatgtg gtacggaaaa tagacattcc
      901 atagttttcc tcttcaacgc cccaacaaag tatgagcttt gttggggcgt tttttattgg
      961 tcgtataatc gacacatgcc cagcgtccaa cttttcatca cctgtctcac cgattcattc
     1021 tttcccgaaa ccggtgaagc cgtcgtagac attctctccc gcctcggcat cagtgtggac
     1081 tttacaccca aacagacctg ctgtgggcag ccgcaattca atgctggctt acgcaatgat
     1141 gcgcgcgcta ttgcagaaca caccatcaaa gtatttgaaa agaccagcgg agacatcgtt
     1201 acgccctctg gttcttgtgc ccataatttt cggcacaatc t
//
LOCUS       DAOREF010000931         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_116412_length_1241_cov_3.334739, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000931 DAOREF010000000
VERSION     DAOREF010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116412_length_1241_cov_3.334739"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1058)
                     /locus_tag="PKI33_15755"
     CDS             complement(177..1058)
                     /locus_tag="PKI33_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNM38526.1"
                     /translation="MSSLSNIAIKLIPASHFSIDELTGIYNQTRVDYMVPMPMNSARL
                     MEYIEFYDVDLDRSVVAIEDGELRGVAMLGVRAERTWVTRLGVIPSTRRGGVGEALMQ
                     SLLEQSARLGIDFTMLEVIKNNVPAHRLFLKLGFYEIGELLILRRAPSKKLVEPVIAD
                     ARRLERYEALDLVGYDRGTQPWTNQSESMSHAQDISGLYLTLQDGSQGWLVYQRQKFT
                     LTHFIYKTEAGDPATMAYAFLSHLHHQYPRLDTHIENIQVNDPHLPGFQKMGYVESFR
                     RIEMWRGNPPSSIMPSK"
BASE COUNT          348 a          292 c          273 g          328 t
ORIGIN      
        1 atcccaatac gatacctacc aagaccgcta ctacttcaac gccgagctca cccgctctat
       61 catggtccgc catggggcgt tggcagagcg tccgtttgcg gaggggtttg atattttgag
      121 gattgtaggg aagttcgaat aatataaaaa ataaaacagg ctttcagaag cctgttttat
      181 tttgaaggca taatcgatga cggaggatta ccccgccaca tttcgatgcg gcgaaaggat
      241 tcaacatatc ccattttctg aaagcccggt aaatgtgggt cgttgacttg aatattttca
      301 atatgcgtat ccaaccgtgg atattgatga tgcagatgcg ataaaaaggc gtatgccatt
      361 gtagctgggt cacccgcttc ggtcttgtaa ataaaatgcg tcagcgtaaa tttctgccgc
      421 tgatacacga gccagccttg gctcccgtct tgaagcgtga ggtagagacc ggaaatatcc
      481 tgcgcatggc tcatcgattc ggattgattc gtccatggtt gagtcccacg gtcatatccc
      541 acaagatcca gtgcttcata tcgttccaat cgacgtgcat ctgcaattac aggttccact
      601 agttttttag atggcgcacg cctaagaatc agcaactccc caatttcata aaacccaagt
      661 ttcaggaaca atcgatgggc aggaacgtta tttttaataa cttccagcat ggtgaagtct
      721 atgcccagtc tggcagattg ctccagcaag ctttgcatca aggcttcacc aacgccaccc
      781 cgccgcgtgg acgggataac acctaaacgg gtgacccatg tccgctcggc acgaaccccc
      841 aacatagcta caccccgtaa ttctccgtct tcaattgcaa caaccgaacg atctaaatct
      901 acatcataaa actcaatata ttccatgaga cgcgcagagt tcatgggcat gggaaccata
      961 taatctacac gagtttggtt gtagatgcca gtcaattcgt caattgaaaa gtgactagcg
     1021 gggattaact taatggcaat atttgataaa gaagacattc ttaagggtta gccgtttaag
     1081 aggaaactct ctacttgagc aataaactca ttcacacgaa taataggttt actaataaaa
     1141 ccattgcagc catagctttc tgccatgttt tttgctgtct cttgaggcca tgcagtaaag
     1201 gcaatgattg gcagtcgttc aaaggttggg ttttcacgaa t
//
LOCUS       DAOREF010000932         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_116799_length_1239_cov_3.389358, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000932 DAOREF010000000
VERSION     DAOREF010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116799_length_1239_cov_3.389358"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKI33_15760"
     CDS             <1..403
                     /locus_tag="PKI33_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNM38527.1"
                     /translation="RTSLALGVGGILLLNMDPVDLYDRRLIRASRGYLFSLPMITAST
                     ADFLDYCKKNNESLMVMDMHADKTVDDIAATPHRLLLAFGSEKDGCSPEIENAATMKV
                     RIPISDKVESLNVSASAGITLYSRSKFNQAN"
     gene            495..911
                     /locus_tag="PKI33_15765"
     CDS             495..911
                     /locus_tag="PKI33_15765"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNM38528.1"
                     /translation="MKLATLPALVFLSLAMFVFNACSNSGTTLTGEWKLVSYGDATNI
                     TPAVANAETSINFNDGTFGGTVGCNSFGADYTSEGSSIHIGSVVSTMMFCEETSAQES
                     AVLGIVSDKTLNVSMNGDRLTLTSEDGKSVIVLEKK"
     gene            complement(944..>1239)
                     /locus_tag="PKI33_15770"
     CDS             complement(944..>1239)
                     /locus_tag="PKI33_15770"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNM38529.1"
                     /translation="VLIALTFWFVKFDNNVTILQALMDSGRYPATVYPAWLRLIVTFI
                     IPIAVATTVPLQALRGELTIVQIFIFLGVSVASFLIASQVWKSGLKQYSGASS"
BASE COUNT          315 a          313 c          313 g          298 t
ORIGIN      
        1 ccgcacctcg cttgcactgg gcgtgggcgg catcctcctg ctcaatatgg accccgtgga
       61 tctgtacgac cgccgtctca tccgcgcgag ccgggggtat ctcttttccc tgcccatgat
      121 caccgcatcc actgcagact ttttggatta ctgcaaaaag aataacgagt cgctcatggt
      181 catggacatg catgcggaca aaacagtgga tgatatcgcc gctacccccc accggctctt
      241 gcttgccttt ggcagcgaaa aagatgggtg ttcgccagag atcgaaaacg ccgccacgat
      301 gaaggtgcgc atccccatca gtgacaaagt cgagtcgctc aacgtttcgg cttcagcggg
      361 aatcacactc tacagccgct caaaattcaa tcaagccaat tagtccaaaa gaaccgaggt
      421 tttcctcggt tctttttttg cgggaacttt ttctccatcg gggcgtcaaa aatatatatc
      481 aaggagaaga aattatgaaa ctcgccacct tacccgctct tgtcttttta tctcttgcca
      541 tgttcgtgtt caacgcctgc tcgaacagtg gcaccaccct tacaggtgaa tggaaactcg
      601 tctcgtatgg ggatgctacg aacattacgc cagccgtcgc aaatgcagag acatctatca
      661 atttcaacga tggaactttt ggcggcaccg taggctgtaa ttccttcggc gcggactata
      721 cgtcagaagg aagcagtatt cacattggct cggttgtttc gaccatgatg ttctgcgaag
      781 aaacctctgc tcaggaaagt gctgtacttg ggatcgtctc tgataaaact ctgaatgtat
      841 ccatgaacgg cgatcgcttg actcttacaa gcgaagatgg aaaatccgtg atcgtgctgg
      901 aaaagaaata aaaaaacggc gaccgtctgg tcgccgtttt catttaactg cttgctccac
      961 tatattgttt gagccctgat ttccaaactt gggaggcaat caagaaactc gcaacactta
     1021 cccctagaaa gataaagatc tgcacgatgg tcaactcgcc gcgcaaggct tggagtggaa
     1081 cggttgtggc tacagcaatg ggaatgataa aggtgacgat gagacgcagc catgccgggt
     1141 aaacggttgc cgggtagcga cctgagtcca tgagggcttg caggatggtg acgttgttgt
     1201 cgaatttgac gaaccagaat gtgagggcga tgaggacga
//
LOCUS       DAOREF010000933         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_117700_length_1234_cov_3.765055, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000933 DAOREF010000000
VERSION     DAOREF010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117700_length_1234_cov_3.765055"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKI33_15775"
     CDS             complement(<1..650)
                     /locus_tag="PKI33_15775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNM38530.1"
                     /translation="MPSLFNRLFHKEKSTSHKIVAIKVGVVVNQRYQLESELGRGGMG
                     IIYKAHDLTENREVAFKVINPETANSLSLNQFAREADILSSFDHPHIVSVHETGFVNE
                     DPSLPFLVMELLHGKPLSEIGTLTYSRIVSIAKQICEALTYIHSQGFVYRDIKPNNIL
                     LEKRGFDYFVKLIDFGLARPLGDAYLPNESSLAGTVFYLAPELIEGQVADVRSDLYA"
     gene            733..>1234
                     /locus_tag="PKI33_15780"
     CDS             733..>1234
                     /locus_tag="PKI33_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNM38531.1"
                     /translation="MRILVVSDIHANFVAFEAVLKDAGSFDTVWCLGDVVGYGPQPNE
                     CVEQLRELNPVCLAGNHDLAVTGQIALWDFTKDAQEVIFWTRHWLTQENMEWLTTLSS
                     TPQVEHGITIVHASLRDPVWEYITTASLVKDNIQFAQTPICLHGHTHLATLFRKKWDE
                     YRILEEY"
BASE COUNT          338 a          273 c          286 g          337 t
ORIGIN      
        1 gcgtataagt cagaccgcac atcagcgacc tgaccttcaa tcaattcggg ggcgaggtaa
       61 aaaacggttc cggcaaggct gctttcgttc ggcagatatg catcccctag cggtcgggct
      121 agcccaaaat cgatcagctt tacgaaataa tcaaatccac gtttttcgag caggatatta
      181 ttcggtttga tgtcacgata cacaaagccc tgactatgaa tatatgtcaa cgcttcacat
      241 atctgttttg cgattgagac aatgcgggaa taggtcaatg tgccgatttc agaaaggggt
      301 tttccgtgca agagttccat cacaagaaaa ggcagtgatg ggtcttcatt gacaaacccc
      361 gtctcatgta ctgaaacaat atggggatga tcgaagctgg aaagaatatc cgcttcacgc
      421 gcaaattggt tcagcgaaag tgaatttgca gtttcggggt tgatgacttt gaaggcaact
      481 tcgcggttct cagttaaatc atgagcctta taaataatgc ccataccacc acgcccaagc
      541 tcactctcca attggtagcg ttggttgaca acaactccaa ccttaatagc aactatcttg
      601 tgcgaagtgg atttctcctt gtgaaagaga cggttaaaaa gagagggcat gttcaaatta
      661 tagcgttgct gtcatctgta aaaaagttac tttcgtcatt ctttctgtac gctataatat
      721 ttttaggtat ccatgcgcat tcttgttgta tcagatatcc atgcgaactt cgtcgcgttt
      781 gaagcggtgt tgaaagatgc cggtagtttt gatacagtct ggtgcttggg cgatgtggtg
      841 ggatatggtc cgcaaccgaa cgaatgtgta gagcaactgc gagaattaaa ccctgtttgt
      901 ttagcgggta atcatgacct tgccgtaaca ggtcaaatcg cgttatggga tttcactaaa
      961 gatgcacaag aggtcatttt ttggacgcgt cactggctca cacaagaaaa tatggaatgg
     1021 ctgacaacct tgtcatctac gcctcaagtt gagcacggca ttaccatcgt ccacgccagc
     1081 ttgcgcgacc cggtctggga atacatcacc actgccagct tggtcaaaga caatatccaa
     1141 ttcgctcaaa cccctatctg tttgcatgga catactcatc ttgctactct ctttcggaag
     1201 aaatgggatg aatatcgcat tctggaggaa tatc
//
LOCUS       DAOREF010000934         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_118714_length_1229_cov_2.341567, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000934 DAOREF010000000
VERSION     DAOREF010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118714_length_1229_cov_2.341567"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..>1229)
                     /locus_tag="PKI33_15785"
     CDS             complement(25..>1229)
                     /locus_tag="PKI33_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38532.1"
                     /translation="HDRNRDGTPEWDHILQTGLEDNPLFDVWNPWSQGMDVSFVHSPA
                     LESMLYREALTLIRIGKKLGKPDEETALIFAQADKLKESLDTSWNSRISYYSYRDRET
                     GVMTEPKVIAKRKGDGSMRPKFESETPVRLLVEIQTKSPAAKRPEVEISEYFTKSKGE
                     TEVIAGHQFQWRTGGLVATTQMTWQRVGRISVVGLEENDKINIKIVDMTGEDITLGLP
                     LWAGALEQQRAYAMIGRNLMTADRFDRPFGMPSMPTVPDKDAEQIALSVSLPWNLLLA
                     EGLLAYGFRAEATRLTAHIMNAIIQNLKQNRAFYQRYHAEKGTGIGERNSLTGFAPVG
                     LFMQALGVRIFSSTRVKLEGRNLFPFAVTIKYKGMSIARGQEQTTVTFANGESVIVKD
                     EAPCIVEM"
BASE COUNT          273 a          320 c          305 g          331 t
ORIGIN      
        1 agacttcgga ggtctaaact aaagttacat ttcaacgata caaggcgctt cgtctttgac
       61 aatcacactt tcgccgttcg cgaaggtgac cgtggtttgt tcttgtccac gcgcaatcga
      121 catgcctttg tatttgatcg tcactgcgaa tgggaaaaga tttcttcctt cgagcttgac
      181 tcgcgtactg gaaaaaatcc tcacacccaa ggcttgcatg aacaaaccca ctggcgcaaa
      241 accggttaac gaatttcgtt cgccaatgcc tgtccctttt tcggcatgat agcgttgata
      301 gaaggcgcga ttctgtttca ggttttgaat gatggcattc atgatgtgcg cagtgagtcg
      361 cgtcgcttca gcgcggaagc cataagcaag caaaccttca gcgagcaaca aattccacgg
      421 taggctcacg ctcaaagcaa tttgttccgc gtctttatct ggcaccgtcg gcatggacgg
      481 cataccaaag ggtctgtcaa atcgatccgc cgtcatgagg ttgcgtccga tcatggcgta
      541 ggcacgttgt tgttcaagcg cacctgccca tagaggcaac ccaagcgtaa tatcttcgcc
      601 agtcatatcc acgatcttga tgttgatctt gtcgttctct tccaaaccta caacgctgat
      661 tctgccaacg cgctgccatg tcatttgagt cgtggcaaca agcccgcctg tgcgccattg
      721 gaattgatgc ccggcgatga cttctgtttc gcctttggat ttggtgaagt actcactgat
      781 ctccacttcc gggcgcttcg cggcgggact tttggtctga atctccacca gtaagcgtac
      841 cggcgtctca gactcgaact tggggcgcat cgaaccatcg cctttgcgtt tggcaatgac
      901 tttgggttcg gtcatcacgc ccgtttcacg gtcgcgatag gaataataac tgatacgcga
      961 gttccaactg gtatctaatg attcttttaa tttatcggct tgtgcaaaga tcagcgcggt
     1021 ttcttcatca ggcttgccga gctttttgcc gattcgaatc aacgtcaacg cttcgcgata
     1081 tagcatggac tcgagtgccg ggctgtggac gaaggaaacg tccatgcctt gtgaccaggg
     1141 attccaaaca tcgaagagcg gattatcttc caaaccagtt tgcaagatgt gatcccattc
     1201 tggggtgccg tcacggttgc ggtcgtgtg
//
LOCUS       DAOREF010000935         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_120885_length_1218_cov_4.443680, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000935 DAOREF010000000
VERSION     DAOREF010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120885_length_1218_cov_4.443680"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1160
                     /locus_tag="PKI33_15790"
     CDS             129..1160
                     /locus_tag="PKI33_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38533.1"
                     /translation="MFILLHTACNGQSTIAPTATNTQTKTLIPTKTPTLISTPTPKAS
                     STSNPVASTEKAGRATAIAIQTAERAASIQAWDAKETQIAEFSIDCDSINMYSSNISP
                     NGKWFSASCDEKETSTLVVQNKVGTKWVLEFKDFLSKDSPEGMWGGVSPVFWSPDGNF
                     LYFTIGLGYSGGGNYCFPKNRGKYGLFRLDLLRGSWSTIISPTDNFPGYEIVFSPTGR
                     RFAISLDGVTISDLNTGENIRLGINGIVERMSWSPDGKYLAYASAICDDENIVSSSIY
                     IWDSTTSQSQILFEADKMILRPEAWTSNSTLRIAGEEIIGFDSAYTIYEYNIEPRGLL
                     FSGTETPNP"
BASE COUNT          396 a          252 c          257 g          313 t
ORIGIN      
        1 gttggacggc ttcgccgtcc ccgccccagc gcaggtaacg caaaccgtta gccgcttggt
       61 tgccaatatt gaaatcaggg aaaataagaa tgaagttgca aaggttgaat ttaattatta
      121 cgtgcttttt gtttatcctg ttacataccg cttgtaatgg tcaaagtaca attgcaccaa
      181 cagccacaaa cacccaaact aaaacactaa ttccaacaaa aacccctacg ctaatttcaa
      241 ctcctacacc aaaggcaagt agtacttcca acccagttgc atccaccgaa aaggctggac
      301 gtgctacagc catagcgata caaacggcag aaagagccgc aagtatacaa gcatgggatg
      361 cgaaagaaac tcaaatagcc gaattttcta ttgactgcga tagcatcaat atgtattcaa
      421 gcaacatttc cccaaatgga aaatggtttt ctgcaagttg cgatgaaaaa gaaacttcaa
      481 ctttagtagt gcagaacaaa gtgggcacaa aatgggtatt agaatttaaa gactttctca
      541 gtaaagatag ccctgaagga atgtggggcg gagtttcgcc agtgttttgg agtcccgatg
      601 ggaattttct ttacttcaca ataggattag gatattctgg gggaggcaac tactgttttc
      661 caaagaatcg tggcaaatac ggtttattta ggcttgacct cttaagaggt agttggtcaa
      721 ctattatctc cccaacagat aatttccctg gatacgaaat tgtcttctca ccaactggta
      781 ggcggtttgc aatatcacta gacggtgtga cgatttcgga cttaaacact ggtgaaaata
      841 ttcgattagg aataaacgga attgttgaaa gaatgagttg gtcaccagac gggaagtatc
      901 ttgcctacgc ttccgcaatt tgtgacgatg aaaacatagt atcatcatcg atatatattt
      961 gggactctac gactagccaa tcccaaatct tatttgaagc agataaaatg atactaagac
     1021 ctgaggcatg gacaagcaat tcaacgctta gaattgcagg agaagaaata ataggttttg
     1081 attcggcata cacaatatat gaatacaata ttgaaccgag aggtttatta ttttcaggga
     1141 cagaaacccc caatccataa cgcggctaac aaagcgtgca cctgacgctg gggattctgc
     1201 gcacatccca agcagttt
//
LOCUS       DAOREF010000936         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_121993_length_1213_cov_2.342832, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000936 DAOREF010000000
VERSION     DAOREF010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121993_length_1213_cov_2.342832"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKI33_15795"
     CDS             complement(<1..687)
                     /locus_tag="PKI33_15795"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HNM38534.1"
                     /translation="MFKTIEDYLDALKNEMKDADPALVQDAQSDAREHLTMALSAAKE
                     KQPDISDADALDSIIDGYGSPEETASAYKEVERRTSPALTQAVKPQSFLVRFFGVYLD
                     PRAWGALFYMLIAFVTGIFYFTWAVTGVSLSLSLMILIFGLPVALLFLISIRGLALLE
                     GRLVEALLGIRMPRRPLFTHQGMKWLERLKALLVDRQTWMMLLYMVLQFVLGIVYFVV
                     IVMVLSFSLAF"
     gene            complement(692..1048)
                     /locus_tag="PKI33_15800"
     CDS             complement(692..1048)
                     /locus_tag="PKI33_15800"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNM38535.1"
                     /translation="MVEAGSHTKKFQKELNAGTSSLVLLSVMSRSDEPMYGYQIAKML
                     EESAPDIPMMKQGTLYPVLRSLEENGLLESTVEPSVSGPPRRYYKITKDGRAALEEWT
                     ELWKQTKKFVDSILKG"
BASE COUNT          364 a          297 c          226 g          326 t
ORIGIN      
        1 aaatgctaga gaaaaactca gtaccattac gatcacaaca aagtaaacaa tacccagcac
       61 gaattgcaaa accatataca gcaacatcat ccaggtttgg cggtcaacca gcaatgcctt
      121 gaggcgttca agccatttca tgccttgatg tgtgaacaag gggcgacgcg gcatacgaat
      181 gcccagtaaa gcttccacca accgtccttc taacaatgct agcccgcgaa tagaaattag
      241 aaaaagcaac gcaaccggta agccaaagat caaaatcatc aaggacagag aaagtgatac
      301 acccgtaact gcccacgtga aatagaagat gcccgttaca aatgcaatca acatgtaaaa
      361 caaggcgccc catgctcgtg ggtcaaggta taccccaaaa aagcggacta aaaaagattg
      421 cggcttgact gcttgcgtca acgcagggga tgtacgacgt tccacttctt tatatgcaga
      481 agccgtttct tccggcgagc catatccatc aataatggag tccaatgcat ctgcatcaga
      541 aatgtcaggt tgtttttcct ttgccgcact taatgccatc gtcaagtgtt cacgagcatc
      601 agactgcgca tcttgaacca aagccggatc ggcatctttc atctcatttt ttaaagcatc
      661 taaataatct tcaatcgtct taaacatgat gtcatccttt caagatcgag tctacgaatt
      721 tcttcgtttg tttccacaat tctgtccatt cttccaacgc ggcacgccca tctttggtga
      781 tcttgtaata gcggcgtggc ggaccagaga ccgaaggctc tactgtgctt tccaacaatc
      841 cattttcttc cagtgaacga agcactgggt ataacgtccc ctgcttcatc attggaatat
      901 ctggggcgct ttcctccaac attttcgcta tctgatagcc gtacatcggt tcatcacttc
      961 ggctcataac actcaaaagt accaaagaag atgtacccgc gttcagttct ttttggaatt
     1021 tcttagtatg tgatccagct tcaaccatat atgctccttt caataatatc aatttcttac
     1081 tactaataaa ttcataatag tatcaatttg atattatgtc aagggtttaa acgaaaaaga
     1141 gccagccatt tggctagctc ttcagtactg tcaaaattat cgatttccat aatcgagttt
     1201 tggcaaagat ctt
//
LOCUS       DAOREF010000937         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_122115_length_1212_cov_5.019879, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000937 DAOREF010000000
VERSION     DAOREF010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122115_length_1212_cov_5.019879"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1212
                     /locus_tag="PKI33_15805"
     CDS             107..>1212
                     /locus_tag="PKI33_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNM38536.1"
                     /translation="MLTSRASRLKYNFSQDFFRPDGHVVFSDISSARAFANQMSALRT
                     APVSASDLYVISLLDEAYHILLRNYYSRYTGVMGRAMASLQSTFGSKYDLTLNKFTEE
                     FPPKAVFRGETTAGVYLATKLPNFGDFGHGVRAAAIEEMLLINNANKNPALAPYMDLF
                     DENVMNGSAFKDFTSHLLEFFSTQPGLGNGAAGKTETLAELLRGPIEASPDSLEGQLK
                     FILDKWGYLLGNEFSNRILRGMDYLREDVIRKQGPADTFNADVPVPTFTGHEYAEFER
                     YSPDQEWMPRLVLIAKNTYVWLDQLSKKYEREIKNLNDIPDEELDQLAARGFTGLWLI
                     GLWERSRASQRIKQRMGQEDAVASAYSLHSYDIA"
BASE COUNT          293 a          302 c          283 g          334 t
ORIGIN      
        1 tagaacacgc caatgaacaa taggacgatg acttgggcta gcgatgtgct catgatgatg
       61 gtttaaattt tactctaatt cattattaat cggttatcat tattgtatgc taacttcacg
      121 tgcctcccgt ctcaaataca atttttcgca agactttttt cgccccgacg ggcatgttgt
      181 tttttcggat atttcctccg ctcgggcatt tgcaaaccag atgtctgcgc ttcgcactgc
      241 ccccgtttct gcctctgacc tctatgtgat ttctctgctc gacgaagcct atcacatcct
      301 actccgcaat tattacagcc gttacacggg tgtgatgggg cgggcgatgg cttccttgca
      361 gtctacattc ggctcgaaat atgaccttac cctgaataaa tttaccgaag aattcccgcc
      421 caaagcggtc ttccgcggtg agacgacagc aggagtgtac ctggcaacca agttgccaaa
      481 ctttggggat tttgggcatg gtgttcgtgc ggcagcaatc gaagaaatgt tgttgatcaa
      541 taacgccaac aagaacccgg cactcgctcc ctatatggat ttgttcgatg aaaacgtgat
      601 gaacggctct gccttcaaag atttcacgtc gcacctttta gagttcttct ccactcagcc
      661 tggtttgggt aatggcgcgg cagggaagac tgaaaccctt gccgaacttt tgcgcggacc
      721 cattgaagcc tccccagact cattggaagg tcagcttaaa ttcattctcg ataaatgggg
      781 atatttgctt ggtaatgagt tttcaaatcg tattttgcgc ggcatggatt atttgcgcga
      841 agatgttatc cgcaagcagg gtccagcaga tacgttcaat gcagatgttc ccgtcccaac
      901 ctttaccggg catgagtatg cagagtttga acgctacagc ccagaccaag aatggatgcc
      961 ccgcctcgtt ctgatcgcga agaataccta tgtttggctc gatcaacttt ccaaaaagta
     1021 tgaacgcgag atcaagaatc tcaatgacat cccagatgaa gaattagacc aactcgcagc
     1081 tcgcgggttt actggtttgt ggctcattgg cttatgggag cgcagccgcg cctcacaacg
     1141 tatcaaacag cgcatgggtc aggaagatgc tgtcgcttcg gcgtattctc ttcattcgta
     1201 tgatatcgcc aa
//
LOCUS       DAOREF010000938         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_122116_length_1212_cov_4.617113, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000938 DAOREF010000000
VERSION     DAOREF010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122116_length_1212_cov_4.617113"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..529
                     /gene="secG"
                     /locus_tag="PKI33_15810"
     CDS             305..529
                     /gene="secG"
                     /locus_tag="PKI33_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNM38537.1"
                     /translation="MSNILDIALIITSVGLILSVILQSKGAGLGGLTGADTGGVFTAR
                     RGIERILFWVTIGLSVVFFALVLTIIFLGK"
     gene            617..>1212
                     /locus_tag="PKI33_15815"
     CDS             617..>1212
                     /locus_tag="PKI33_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNM38538.1"
                     /translation="MKRLRWQILVVAVTLVIVALLLLSQQPVSVVTLPQAAPGGIYTE
                     ALIGSMGRLNPMLDWNNSADRDVNRLIFSGLIKFDSRGLPRPDLIDSWAASADGKVYN
                     FTLRKNAVWHDGQPVTSDDVLYTIEVIKSSASLFPQDIKDLWSQIEVKRLDDKTFQFI
                     LPEPFSPFLDYVTFGILPKHILESIPADQLPTAEFNLKP"
BASE COUNT          258 a          323 c          301 g          330 t
ORIGIN      
        1 aatagccttc aaagccttgc acgagaatat tatgatcggt gacgatgacc acatccacac
       61 cggctttgag cccggcggcg gcaatgtctt tgtgaagtcc gctgccatca gagtagcggg
      121 tgtgcatgtg caggttgacg ataacttcgt gcattcaagt cctcttgttg taatagacct
      181 gacaggtctt tgaaatctgt caggtctgaa gttttactgc gggtgagagg tttttaagct
      241 cgtttgacga ttcacttctc gtacaggtat aatttgcccg ctaaattcta ggaggcactt
      301 tcgcatgtca aacattttgg atattgcttt gattataacc tcagtgggtt tgatcctcag
      361 cgtcatcctg cagagcaagg gcgcagggtt gggtggtctc accggcgcgg acacgggtgg
      421 tgtattcacc gctcgccgcg gcattgagcg tattctgttc tgggtgacga ttggtttgag
      481 cgtcgttttc ttcgcgctcg ttctaaccat tatcttcctt ggaaaataag aattccctgc
      541 ctgaaaggcc cgccgttttc aaatgcccgc accccgggca gggcggtcct ttcttttttt
      601 cccacaacaa aacattatga aacgattacg ctggcagatt ctggttgtgg cggtaacgct
      661 tgtgattgtg gcgttgctct tgctcagcca acagccggtt agtgtagtga ccttgcccca
      721 ggcggcgccc ggcggtattt ataccgaagc gctcattggc tctatgggtc gcctcaaccc
      781 gatgctggac tggaacaact ctgccgatcg agatgtgaac cgcctcattt ttagcggttt
      841 gatcaaattc gattcccgcg gcttgccccg cccagacctg atcgattcat gggcagcctc
      901 ggcagacggc aaggtctata acttcacctt gcgcaagaac gccgtctggc acgacggtca
      961 accggtgacc agtgatgatg tgctttacac catcgaggtc atcaagagtt cggcttcgct
     1021 cttcccgcaa gatatcaaag acttgtggtc gcagatcgaa gtaaaacggc tcgacgataa
     1081 aaccttccaa ttcattctcc ccgaaccgtt ctctcccttt ttggattacg tcacctttgg
     1141 cattttgcca aagcatattt tggaatccat ccccgccgac caactcccaa ccgcagagtt
     1201 taacctcaag cc
//
LOCUS       DAOREF010000939         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_124196_length_1202_cov_3.750654, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000939 DAOREF010000000
VERSION     DAOREF010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124196_length_1202_cov_3.750654"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKI33_15820"
     CDS             complement(<1..269)
                     /locus_tag="PKI33_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38539.1"
                     /translation="MKKISLFLFIAILFSACAPATEAPALPAEPTVSAQPTSTQMPVA
                     DALWVSPAVPNVLREAAKSSGVPIVNDAETTTQKLDIADSGSLWI"
     gene            complement(373..>1202)
                     /gene="menC"
                     /locus_tag="PKI33_15825"
     CDS             complement(373..>1202)
                     /gene="menC"
                     /locus_tag="PKI33_15825"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNM38540.1"
                     /translation="IRGHHLAKAGVEMAIWDLLGKRQGKSLKELFGGVRDKVEMGVSV
                     GIQETPEKLVETVTNFVNSGYARVKLKIKPGKDVTYAQAIRKAFPNIRLQVDANSAYS
                     MDDADSLKPLDDLNLLLIEQPLFEDDIWDHHKFQAKFSTPVCLDESIVSPRHARYAIE
                     MKACRIINIKAGRLGGLSQGIQTHDISQQNGMPVWCGGMLETGIGRASNLALSSMPNF
                     KLPGDVSASDRYYTRDITHERFVLNSDSTIDVPNKPGLGVTVDEEALKSYTLAKVVV"
BASE COUNT          302 a          266 c          314 g          320 t
ORIGIN      
        1 taaatccaaa gagagcctga atcggcaatg tctaattttt gagtagtggt ttcagcatca
       61 ttcacgattg gcacaccaga acttttggca gcttctcgca agacattggg cacggcaggg
      121 ctaacccaca atgcatctgc aaccggcatt tgcgtggaag ttggttgagc agagacagtt
      181 ggttcagcgg gcaaggcggg tgcttcagta gcaggcgcac aagcagagaa tagtattgca
      241 atgaagagga atagtgatat ttttttcatt tgtaatccat ggcgcaaaaa agtacgaagc
      301 acagagcgcc tcgtacttgt aaacttaatt gattaaacgg gcgtgagcgt tagtttacac
      361 ccagtttggt tgttagacta ccacctttgc caacgtgtaa gacttgagcg cctcttcatc
      421 gacagtgacg cccaaaccgg gtttgtttgg tacgtcgata gttgaatctg aattgagaac
      481 gaaacgctca tgcgtgatat cgcgagtgta gtaacggtca gatgcggaga catcaccggg
      541 caatttgaaa ttcggcatgg acgataaagc aagattcgaa gcgcgaccaa taccggtctc
      601 taacatgccg ccacaccaaa cgggcattcc gttttgttga gaaatatcgt gcgtttgaat
      661 gccttggctt aatccgccga gcctgcctgc tttgatgttg atgatgcggc aggctttcat
      721 ttcgatggca tagcgagcat ggcgcggaga gacgatgctt tcatcaagac aaactggggt
      781 actgaacttg gcttggaatt tgtgatgatc ccaaatatca tcttcaaaga gcggctgttc
      841 aatgagcagt aagttcaaat catcgagcgg tttgagtgag tcggcatcgt ccatggaata
      901 agcagagttg gcgtccactt gcaagcggat gttcggaaat gctttgcgaa tggcttgggc
      961 ataggtcaca tctttgccgg gtttgatttt gagtttcacg cgggcatacc cgctgttgac
     1021 aaagtttgtg acggtttcca ccagtttttc aggtgtctct tgaataccga cggatacacc
     1081 catttccact ttatctcgca cgccgccgaa taactctttg agtgatttgc cttggcgttt
     1141 cccgagcaaa tcccaaattg ccatttctac accggctttg gcaaggtgat gtccgcggat
     1201 gc
//
LOCUS       DAOREF010000940         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_124580_length_1200_cov_4.303057, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000940 DAOREF010000000
VERSION     DAOREF010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124580_length_1200_cov_4.303057"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKI33_15830"
     CDS             complement(<1..201)
                     /locus_tag="PKI33_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38541.1"
                     /translation="MSTNPITQETSLKNRPGLRWTLLGVWGLVVLYTIMVLGAVLSPQ
                     FMMFRDASMARILLGIPALLQTI"
     gene            383..772
                     /locus_tag="PKI33_15835"
     CDS             383..772
                     /locus_tag="PKI33_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNM38542.1"
                     /translation="MATYTQNMLDYSEQIRNVTQYIREHINESLSREVLAEVAGFSVP
                     HFHRVFIATTGESAASYVRRMRLERAGRKLRMGAVDITEVALAAGYVSHAAFGKAFKQ
                     QFGLSPSEFRQLSCSHATQLLTRGMKK"
     gene            769..1167
                     /locus_tag="PKI33_15840"
     CDS             769..1167
                     /locus_tag="PKI33_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNM38543.1"
                     /translation="MKIKLASVSIDEYDKALKFYTEVLGFILKRDIPLGNDARWITVV
                     SPDDPNGVELVLEPNASYPAMKALKESLVRDGIPFTAFEVKDIQKEYERMKKLGVEFT
                     MEPTNMGTTTAAIFDDTCGNLIQIYQLMDM"
BASE COUNT          350 a          259 c          283 g          308 t
ORIGIN      
        1 aatggtttgc aaaagcgcag ggatgccaag caaaatgcga gccatgctcg catctctaaa
       61 catcataaac tgtggcgaaa ggactgcccc caacaccata atagtgtaga gcacaaccag
      121 tccccaaacc ccaagcagcg tccaacgcag tccgggacga tttttcagag aggtttcttg
      181 tgtgataggg ttcgtggaca taagttgccg ttgattttca aaatcataac tccaaaaccg
      241 cttcacactc tttttatttt tggaaggtgg tcggggttta gtcaacttac gctgtagcag
      301 gcgaaaagtt gcctattttt tctaaggttc atgatacatt tggagtagcg agaccctctt
      361 tctctgaaat atattgagcc gcatggcgac ctacactcaa aacatgttgg attattctga
      421 gcagattcga aacgttaccc aatatatccg tgagcatatt aatgaatccc tgagccgtga
      481 agtattggca gaggtggcag ggttctctgt gccgcatttt caccgcgtat ttattgccac
      541 cacgggagag agtgcagcaa gttacgtccg ccgcatgcgg ctcgaacggg cggggcgaaa
      601 acttcgcatg ggcgccgtgg atatcaccga agttgcattg gcagcagggt atgtctctca
      661 tgcggcattt gggaaagctt tcaagcaaca gttcggtctg agccccagtg aattccgtca
      721 gttgagttgt agccatgcca cacaactatt aacgagagga atgaaaaaat gaagatcaaa
      781 ttagcaagtg tatctattga tgagtacgat aaggctctaa agttctatac cgaagtttta
      841 gggttcatcc taaaacggga tattcccctt gggaatgatg cccgttggat taccgtagtc
      901 tcaccagatg atcccaatgg cgtggagtta gtccttgagc cgaatgctag ttatcctgcc
      961 atgaaagctc ttaaagaatc acttgttagg gacggtattc cattcaccgc atttgaagtt
     1021 aaggatattc agaaagaata cgaacgcatg aagaaactag gggtcgagtt caccatggaa
     1081 cctactaata tgggaacaac gactgctgct atatttgacg atacttgtgg aaatctaatc
     1141 caaatttatc aactgatgga catgtaatag aaagcaaaaa ggactactat aaaaacacac
//
LOCUS       DAOREF010000941         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_125415_length_1196_cov_3.502191, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000941 DAOREF010000000
VERSION     DAOREF010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125415_length_1196_cov_3.502191"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKI33_15845"
     CDS             <1..305
                     /locus_tag="PKI33_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNM38544.1"
                     /translation="LFTNQTGENVGAFDDRHLEAFADELGLDRGQYDDCRSSGKYSDK
                     AEQDAKDATAAGIQATPSFIITYDVNGETQTRVIQGAQGIDVFAQEIEAALADMGK"
     gene            complement(354..1022)
                     /locus_tag="PKI33_15850"
     CDS             complement(354..1022)
                     /locus_tag="PKI33_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM38545.1"
                     /translation="MRHHRTSILIADDHEVVRSGIRSYLEKISDFQVVGEASSGEETL
                     SLVSELIPDIVLLDLILPGMDGIETTRRIRQISPRTKVVVLTSYHEDVHIFPALKAGA
                     ISYILKDMKMEKLVEVLHRAVQGEVTLHPVVASRVLQNIRGENGEEQPLFTELTDRET
                     DVLKLIAAGLTNGQIADKLFISENTVKGHVSNILSKLHLADRTQAAVYAWQQGIVNRQ
                     STTK"
BASE COUNT          307 a          270 c          284 g          335 t
ORIGIN      
        1 tgcttttcac caatcaaacg ggtgagaatg ttggcgcctt tgatgaccgc catttggaag
       61 cctttgcaga tgaacttggc ttggaccgcg gtcaatatga tgactgtcga tcttctggca
      121 agtattctga caaagcggag caagatgcca aagatgccac agcggcaggc attcaggcaa
      181 cgccttcatt tatcattacc tatgatgtga atggtgaaac ccaaactcgg gtgatccagg
      241 gtgctcaagg gatcgatgta tttgctcaag agattgaagc cgctttagcg gatatgggta
      301 agtaaatttc ataaatacaa aaagacaccg ctttcaaagc ggtgtctttt tgtttatttc
      361 gtggtgcttt gacgattgac gattccttgt tgccaagcat agaccgctgc ctgtgttcga
      421 tctgcaagat gcagcttgct aagaatattg ctgacatgtc ccttgaccgt attttcactg
      481 atgaacaatt tatcagcaat ctgtccattg gtgagcccgg ccgcaattag tttcagtaca
      541 tcggtttcgc gatcggtcag ctcggtgaag agcggctgtt cttcaccatt ttcgccgcga
      601 atgttttgaa gaacacgtga agcaaccact gggtgaagtg ttacttcgcc ttgcacagca
      661 cgatgcagaa cttcaactaa tttctccatt ttcatatctt tcagaatata agagattgcc
      721 ccagctttca atgcagggaa gatatgcaca tcttcatggt aagaagttaa cactactact
      781 tttgtgcgcg ggctgatttg ccgaatgcgg cgtgtagttt cgatgccatc cataccggga
      841 aggatcaagt caagaagaac aatgtcgggg attaactcag agaccaaact cagagtctct
      901 tctccggatg aagcttcacc tacaacttga aaatctgaaa ttttttcaag gtaagaacgg
      961 atgccacttc gcactacttc gtggtcgtct gcgatcaaaa tacttgtgcg gtgatgtctc
     1021 atgttaacct cctgaattgg ctttgagagg gatttgaata ataatacgcg ttccctgccc
     1081 gggcgcactt tggatttgga aggtgccgcg tacactgata atacgttctc gcatggaacg
     1141 gaaacccaag ccggtcgctt tttggttgac attgaacccg caaccatcgt ctgcca
//
LOCUS       DAOREF010000942         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_126310_length_1192_cov_3.483729, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000942 DAOREF010000000
VERSION     DAOREF010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126310_length_1192_cov_3.483729"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKI33_15855"
     CDS             <1..384
                     /locus_tag="PKI33_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38546.1"
                     /translation="ALLSGPLTGDVTSDRALIYKAFAAGHCFVGYDLPAPTKGFRFSA
                     QGQNESVIMGDEISGKNGVTLQAKFPFAAEIILYKDGLPLQTWKNQQACTHITTEPGV
                     YRVEAYRNYLGKRRGWIYSNPIYVK"
     gene            480..1151
                     /locus_tag="PKI33_15860"
     CDS             480..1151
                     /locus_tag="PKI33_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNM38547.1"
                     /translation="MRLSVLRVINIMRISVYVDGFNFYHGLTENTKYRWCDLLKLTKQ
                     FLPNGEYGKIKLFAAHSKEYYDNLGKPERQNRYLQALKTIPGFEFIPSKFSQFKKTLP
                     LRDSPKDKPEFVHVRGYKEKGADVNLASHLISDGIKNLYDIAVVFTNDSDLVEPIRVV
                     RYDLNKKVYILLTCKNRNAWVSKELITVAKYHKLVEEDHLRVSQLPDVVTRPGKPALK
                     KPLSW"
BASE COUNT          365 a          251 c          248 g          328 t
ORIGIN      
        1 gcgcttctct ctggacctct gaccggcgac gtgaccagtg accgtgccct catctataag
       61 gcgttcgctg ccgggcattg tttcgtgggc tacgacctgc ccgcccccac gaagggattc
      121 cgcttctcgg cgcagggaca gaacgaatcc gtcatcatgg gcgatgaaat ctccggcaaa
      181 aacggtgtga ccctgcaagc caaattcccc tttgcggcag agatcatctt atacaaagac
      241 ggtttgcccc tgcaaacttg gaagaatcaa caagcctgca cccacatcac caccgaaccc
      301 ggcgtctacc gcgtggaagc ctaccgcaat tacctaggca aacggcgcgg ctggatctat
      361 agcaatccga tttatgtgaa gtaaatagaa caaatttact gttgactttt tttgagcaat
      421 ttatataata tgtaaagacg tcccccagtc ctcagcggct gggcatgcag gagccgctta
      481 tgcggctctc tgttttgagg gtaattaata ttatgcgaat ttcagtatat gtagatggtt
      541 ttaatttcta tcacggattg actgaaaaca caaaatatcg ttggtgtgac ctattaaaac
      601 taactaaaca atttcttcct aatggggaat atggaaaaat taagttattt gctgctcact
      661 ctaaagaata ttatgataat ttagggaaac ctgaaagaca aaatcgatat ttacaggcat
      721 tgaaaactat accgggcttt gagtttattc ccagtaaatt ctcgcaattc aaaaagacat
      781 tacccttaag agactctcca aaagacaagc cagaatttgt tcatgttcgt ggatataagg
      841 aaaaaggggc ggatgttaac ttagcatctc acttaatttc cgatggaatt aagaatctat
      901 atgatattgc agttgtattt actaacgact cagatttagt tgagccaatt agggttgttc
      961 gctatgattt gaataaaaaa gtttacatct tattgacttg caaaaatcgc aatgcatggg
     1021 taagtaaaga gttgataact gtagcgaaat accacaaact tgtagaagaa gatcatctaa
     1081 gagttagcca attacctgat gtagtaacaa gacccggaaa gcctgcacta aaaaagccac
     1141 tctcttggta gtgtagaaat tctttattaa acttttaccg ttggcttaaa gt
//
LOCUS       DAOREF010000943         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_126715_length_1190_cov_4.049339, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000943 DAOREF010000000
VERSION     DAOREF010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126715_length_1190_cov_4.049339"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..>1190)
                     /locus_tag="PKI33_15865"
     CDS             complement(171..>1190)
                     /locus_tag="PKI33_15865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNM38548.1"
                     /translation="ARNEETNIRRCVEAVLRQDYPNFEVIVLDDRSTDATLTLLKEIA
                     ARDSRLLPISGSDLPEGWAGKPHALYQASAVAKGEWLCFVDADTFLAPNALSAVYAKA
                     SETKADLFTVMTKQILGSFWERTVMPLVMTALSVGFSPRKVNDPSRHDAVANGQFIFI
                     KRSIYDLIGGHEKIKDQIVEDKALSENVKWNGHRLVVANGMQLISTRMYTSLETMWEG
                     WTKNIYLGLRDHPSLLLLGAFGATLALLAALFLPVWPLLGLGWFLNSGGWMALAVIVE
                     ALFVWGYLIVIRARIAQEMDIPRWYAWTTPLGAGVFAAMMLTSAWKVISGQGVTWRGR
                     NYKPK"
BASE COUNT          312 a          318 c          278 g          282 t
ORIGIN      
        1 aagcctcagc cagttcttgg attgaggcag ttttatgtgt tttgagatgt tcaagaatct
       61 tttgttcgcg gtctaaggag ttggtagcca tatgttatat tttgttatat ttaacgagag
      121 attacgaaat ataacaactc aaccagtaat tatcaagatg acatttatca ctactttggc
      181 ttgtaattcc gtccacgcca tgtaacccct tgccccgaaa tcactttcca cgccgaggtc
      241 aacatcatgg ctgcaaacac ccctgcccca agcggagtgg tccacgcata ccagcgcgga
      301 atgtccatct cttgcgcaat tctggcgcgg atgacaatca aatagcccca cacaaataat
      361 gcctcaacaa tcacagccaa tgccatccac ccaccagaat tgagaaacca gcccaagccc
      421 aacaacggcc acaccggaag gaataatgcg gcgagcaatg ccaaggttgc accaaatgcg
      481 ccgagcaata acaacgatgg atggtctcgc aacccaaggt agatgttctt cgtccagcct
      541 tcccacatgg tttccaaaga cgtgtacatg cgagtgctaa tcagttgcat gccatttgcc
      601 accaccaggc ggtgaccatt ccacttcaca ttttcagaga gcgctttatc ttccacaatt
      661 tgatctttaa ttttttcatg cccgccgatc aaatcataga tactgcgctt gatgaagatg
      721 aactgtccgt tcgccactgc atcatggcgc gatgggtcat tgaccttgcg cggagaaaag
      781 cctactgaga gcgcggtcat caccaacggc atcaccgtcc gctcccagaa ggaaccgagg
      841 atttgcttcg tcatcacggt gaacaaatcg gcttttgttt cgctggcttt ggcatacacg
      901 gcggaaagcg cattcggcgc gaggaaggta tcggcatcta caaagcaaag ccactcgcct
      961 ttcgccacag cagaggcttg gtacaaagca tggggtttgc ccgcccagcc ttcgggtaag
     1021 tctgagccgc tgattgggag gagacgagag tcgcgggctg cgatttcttt taatagagtc
     1081 agagtcgcat cagtggagcg atcgtccaaa acgatgactt caaagttggg gtaatcttga
     1141 cgaagaaccg cctcaacaca acgccgaatg ttggtctctt cgttgcgagc
//
LOCUS       DAOREF010000944         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_127640_length_1186_cov_3.236074, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000944 DAOREF010000000
VERSION     DAOREF010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127640_length_1186_cov_3.236074"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKI33_15870"
     CDS             complement(<1..1166)
                     /locus_tag="PKI33_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNM38549.1"
                     /translation="MLTVSRILSGSWQSTSSKIFLPIILVVLLSVQVLTRYGYVRGAG
                     RVAISMLWLSLSIQAYFSDGLRDVTVLAFPILVLLSALLLGWQTGLLTGIASILVIWL
                     FAFQENIGIRTYSIDPPINYAGDLSVVFIITSLLTYLLIQSLNRSLSAAKLELRERLR
                     AEEKLQTQANYLTALHETTFGLLNRLELKPLLETILLRTAHLLETPHVGIDLASVDGV
                     ALRQEMGIGIYEQWNGNYTEKGEGLTGKVWESGNTIVVDDYETWEGKIPLVSGMGLRS
                     VLGAPLKSGDKILGVLLVASDKPKYFTSEKVTLLERLAALASLAIDNARLYESAQKEI
                     RERKLVEDDLRSSEERFRKVFNNSSVAIAIVTLDDGIFLEANQAFWELAGRTPQ"
BASE COUNT          356 a          291 c          247 g          292 t
ORIGIN      
        1 tcttgagggg tgcgtcctgc caattcccag aacgcctgat tcgcttccaa gaatatgcca
       61 tcatccaatg tcacaatggc aatcgccaca ctgctattat taaacacttt acgaaagcgc
      121 tcttcgctgg accgtaggtc atcttctaca agtttgcgtt cacgaatctc tttttgggcg
      181 ctttcataca gacgggcatt atcaattgcc aaagaagcaa gtgccgccag ccgttcgagc
      241 aaggtcactt tttcagaagt aaagtatttt ggtttatcag aagcgactaa gagcacacca
      301 aggatcttgt cacctgattt taatggggca cctaaaacag aacgtaaccc cattcctgat
      361 accaatggga ttttcccttc ccatgtttca taatcatcaa caacaatcgt gtttccgctt
      421 tcccaaactt tccctgtcag tccttcgcct ttttcagtgt agttgccatt ccactgttca
      481 tagatgccga ttcccatctc ttggcgaagg gcaacaccgt ccacagaggc aaggtcaatg
      541 cctacatggg gagtttctaa tagatgtgcc gtacgaagga gaatcgtttc aaggagaggc
      601 ttgagctcaa gacggttgag caagccaaag gtcgtttcgt gaagcgctgt gagatagtta
      661 gcttgggtct gtaatttttc ttctgcgcgc aaacgttcac gcaattccag tttcgccgcc
      721 gaaagggaac ggttcaaact ctggatcagc aaatacgtca ataagcttgt aatgataaat
      781 acaaccgaca agtcaccggc gtaattaatg ggagggtcaa tagaataggt tcgaatacct
      841 atattttctt gaaacgcaaa gagccatatt actaagatac tggcaatccc tgtaagcaag
      901 cctgtctgcc aacccaataa caaagcagac aatagcacca agatagggaa tgccaacacc
      961 gtaacatcgc gcaaaccatc tgaaaaatag gcttggatgc tcaatgatag ccacaacatg
     1021 gagattgcca ccctacctgc tccacgaaca tacccataac gggtcagtac ttgcaccgac
     1081 agtaaaacaa ccaagataat gggaagaaaa attttagagg atgtgctttg ccatgacccc
     1141 gataagatgc gcgatacagt caacaagaaa actacaacaa acgtaa
//
LOCUS       DAOREF010000945         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_128044_length_1184_cov_3.438441, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000945 DAOREF010000000
VERSION     DAOREF010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128044_length_1184_cov_3.438441"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKI33_15875"
     CDS             complement(<1..818)
                     /locus_tag="PKI33_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38550.1"
                     /translation="MKNTTEKLLEIRGLKKYFEAGRASLFSRETKHVHAVDGVNLTLR
                     RSEVIALVGESGCGKSTLGLLLMGLEEPTDGSVIFEERDITHLNDHQRKDLRRKIQMV
                     FQDPYESLNPTQTIEEIVTEPLTVHGIASSHQDRDERVRKAMEDAGLKPADVYLHRYP
                     HELSGGQRQRVVIASALVLEPEIIIADEPVSMLDVSIRAEVINLLAELRISRQIAVIF
                     ITHDLGSVGFFADRVAVMYLGRIVEIGTMLDVLEKPQHPYTKALLSVIPVPNPR"
     gene            complement(802..>1184)
                     /locus_tag="PKI33_15880"
     CDS             complement(802..>1184)
                     /locus_tag="PKI33_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM38551.1"
                     /translation="LSINYVTEGKAPARAVENVSFTLKAGELIGLVGESGCGKTTLML
                     ALLKLLPSAGQIVNGKVFYSGQDITAMNEDEINKVRWSGVSIVFQGAMNALNPVRTVG
                     DQIGEAILKHEPSFPKEKINEEYY"
BASE COUNT          309 a          296 c          258 g          321 t
ORIGIN      
        1 aaacgcggat ttggcacagg gataaccgaa agcaacgctt tagtataagg atgctgtggc
       61 ttttcaagga catctaacat tgtaccaatc tctacaatgc gaccaaggta catcactgcc
      121 acacgatctg cgaaaaagcc aacagaaccg agatcgtgtg taataaagat aaccgcgatc
      181 tggcgcgaaa tgcgaagctc tgccagaaga ttaatcactt ctgcacgaat tgatacatcc
      241 aacatcgaca cgggctcgtc ggcaataatg atctctggct caaggacaag tgccgaagca
      301 atgaccacac gctgtcgttg accgcctgaa agttcgtgcg ggtaacgatg caaatataca
      361 tctgccggtt ttagcccggc atcttccata gcttttcgca cacgttcatc acggtcttga
      421 tgagatgatg caatgccatg cacggtcaac ggttcggtta caatctcttc aatggtctgt
      481 gtggggttca aagactcata cgggtcttga aaaaccattt gaatttttcg gcgcaggtct
      541 tttcgttgat ggtcgttgag gtgagtaata tctcgttctt caaaaatgac cgaaccatct
      601 gtgggttctt ccaatcccat taacaacaag cccaaagtag attttccgca tccgctctcg
      661 cctacaagcg caatcacttc gctgcggcgc aatgttaagt tcacgccgtc cactgcatgg
      721 acgtgctttg tctcacgtga aaacaaagag gctcgcccag cttcaaaata ttttttcagc
      781 ccgcgaattt ccaagagttt ttcagtagta ttcttcattg atcttttctt ttggaaaaga
      841 aggctcatgt ttgagaatgg cttcgccaat ttgatcccca acagtgcgta cagggtttag
      901 ggcattcatc gctccttgaa agacgattga gacaccactc caacgaacct tattgatttc
      961 atcttcgttc atagcggtga tgtcttgtcc gctatagaaa acttttccat tcacgatttg
     1021 cccggcagaa ggtaatagtt tcaacaaagc caacatcagt gttgttttgc cgcagccgct
     1081 ctctcccacc aaaccgatta gttcccctgc cttcagtgta aagctgacat tttctactgc
     1141 ccgcgccggg gctttgcctt cggtgacata gttgatagag agat
//
LOCUS       DAOREF010000946         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_128304_length_1183_cov_2.533688, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000946 DAOREF010000000
VERSION     DAOREF010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128304_length_1183_cov_2.533688"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKI33_15885"
     CDS             complement(<1..568)
                     /locus_tag="PKI33_15885"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNM38552.1"
                     /translation="MTEIEIQETIVEFEHSRHLNFSGTKNFRDLGGYPTMDGRSIRWG
                     VLYRSDSLHRLSEADLRKLASLKLSRIFDFRSENEVHHEPDRLPAGVKLVHLPMLDAS
                     TRVWHEQRADMVKNMKTLNPAEFMFATNMELASKFTPEYKRFFQELLESKGSPILFHC
                     AAGKDRTGFAAAALLRILGVPQDIIMQDY"
     gene            709..>1183
                     /locus_tag="PKI33_15890"
     CDS             709..>1183
                     /locus_tag="PKI33_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38553.1"
                     /translation="MKHRSIQELEAGLPLIKQTPKQEGKLELIVRRPSVEEREIISQG
                     KVDLTIGLEGDNWQARGSKTTPDGSANPETQITLMNSRVIHLLTQDENLWQWAGDQLY
                     VDLDLSEENLPAGTKLSIGTAVIEVTATPHTGCKKFSARFGLDALKFISASENKSL"
BASE COUNT          368 a          263 c          238 g          314 t
ORIGIN      
        1 agtaatcttg catgataatg tcttgcggca caccaagaat tcgaagaaga gctgcggcgg
       61 caaatccagt gcggtctttt ccggctgcac aatgaaacaa aattggactc ccttttgact
      121 ctaatagttc ttgaaaaaaa cgtttatatt caggtgtgaa tttagatgcc aattccatat
      181 tagtagcaaa cataaactcg gctgggttga gcgttttcat gttctttacc atatccgctc
      241 gctgctcatg ccatactctt gtgctagcat ctaacatagg aagatgtact aatttaactc
      301 ctgcaggcaa gcggtctggc tcatgatgta cttcattttc agaacgaaaa tcaaatatcc
      361 gagataattt caaagaagct aattttctta aatcagcctc ggaaagccta tgtaaactat
      421 cagaacgata tagaacgccc caacgaatcg accgtccatc cattgtagga tagccaccaa
      481 gatcacgaaa gtttttagta cccgaaaaat ttaggtgccg cgaatgttcg aactctacaa
      541 tagtttcttg tatttctatt tcagtcatat aaaatgccct ttcaaaagcg tctttattat
      601 ataagctggg ctaagcgcaa cattaacaat tatttatact atatctaaca tccgcttaag
      661 accgggctat aatttaaatc attttttaga gattggagat acaaagtagt gaaacaccgt
      721 tccatacaag aacttgaagc agggctacct cttatcaagc aaaccccaaa gcaggagggt
      781 aaactggagt tgatcgtccg tagaccttct gttgaagaac gcgaaattat ctctcaaggc
      841 aaagtggact taaccattgg cttggaaggc gataattggc aagcccgcgg cagcaaaact
      901 acgccggatg ggtctgccaa cccggagaca caaattacct tgatgaattc gcgcgtgatc
      961 cacttgttga cccaagatga aaacctttgg caatgggcag gtgaccaact ttatgtggac
     1021 ctagacctta gtgaagaaaa cctaccagca gggacaaaac tttcgatagg cacagcagtt
     1081 atagaagtga cagctacccc ccacacgggt tgcaagaaat tctctgcccg cttcgggttg
     1141 gatgctttaa aatttatcag cgcctctgaa aacaaatcgc tac
//
LOCUS       DAOREF010000947         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_129530_length_1177_cov_4.221925, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000947 DAOREF010000000
VERSION     DAOREF010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129530_length_1177_cov_4.221925"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKI33_15895"
     CDS             <1..333
                     /locus_tag="PKI33_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNM38554.1"
                     /translation="AQQIITNGYVSRPFMGINYQAISPNIAAMYNLPVKWGIYVQAIA
                     DGSPASTSGLQKGDIIASLNGVQLDETHQYLNMLYNYKPGDTVTLGIYRDGKQLDLNI
                     TLGENNPT"
     gene            complement(380..>1177)
                     /locus_tag="PKI33_15900"
     CDS             complement(380..>1177)
                     /locus_tag="PKI33_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNM38555.1"
                     /translation="EARRRGTQKTVLERRAPPTFDVLIEIQTRDRFTVHTDIMSAVDS
                     LLRDAPIPPEVRTRDESGEIKIEKAAPAPKSRSEAPTKTPLRTRRQPASQPVDHSPTP
                     SQVPNVGAKLQTIRIFAYGVARNRLQQAAKRLGVPAVVVTDEAEADVLVTLRTYYRNR
                     EHTIMDAEARGMPIYVLRANSVSQVEQFVSDLYNLTEHVQRDNMEDIRDQAKQAISAV
                     LNGERFVDLPPGPSLVRRLQHEMARDAELVSHSYGKEPRRHVRIFRE"
BASE COUNT          282 a          329 c          302 g          264 t
ORIGIN      
        1 gcgcaacaga tcatcaccaa tggctatgtc tcacgtccgt tcatgggcat taattatcag
       61 gcgatctccc cgaacatcgc cgccatgtat aacctgcctg tgaaatgggg catctacgtc
      121 caagccattg cagatggaag ccctgccagc acatcgggat tacaaaaagg cgatatcatc
      181 gccagcctga acggtgttca attagacgaa acgcatcagt acttgaacat gctctataac
      241 tacaaaccgg gcgataccgt caccctcggc atttaccgcg acggcaaaca actcgacctg
      301 aacatcacgc tcggcgaaaa caacccaaca taaaatttct tataaaaata aaggtgacct
      361 atacaggtca cctttatttt tattctctaa aaattctcac atgcctacgc ggttctttcc
      421 cataggaatg agaaaccaat tcagcatcac gcgccatttc atgttgcaaa cggcgcacga
      481 gagaaggtcc gggcggcagg tcaacgaaac gctcgccatt caagaccgca ctaatggctt
      541 gctttgcctg atcacgaatg tcttccatat tgtcacgttg cacatgctcg gtcaggttat
      601 acaggtcact cacgaactgc tctacctgcg agacagaatt tgcacgcaag acatagattg
      661 gcataccgcg cgcctcggca tccatgatgg tgtgctcgcg gttacggtaa tacgtgcgca
      721 gcgtcaccaa cacatcggct tcggcttcat cagtcaccac tacggcgggc acgccgagtc
      781 gcttcgccgc ttgctggagt cgattgcgcg ccacaccata ggcgaagatg cggatggtct
      841 gcaattttgc gccaacgttc ggaacctgac ttggcgtagg cgaatgatcg acgggttgag
      901 aggcaggctg tctacgagtc cgaagcgggg ttttcgtcgg cgcctcactt ctggactttg
      961 gagcgggtgc ggctttctcg atcttgatct cacccgattc gtcgcgagtg cgcacttccg
     1021 gtgggatagg cgcgtcgcgc aagagcgagt caactgcaga catgatatcc gtgtggacag
     1081 tgaatcggtc gcgggtttgg atctcgatca gcacgtcaaa ggttggcggc gcacgacgtt
     1141 cgagcacggt cttctgcgtg ccacggcgac gagcttc
//
LOCUS       DAOREF010000948         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_130189_length_1174_cov_3.592493, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000948 DAOREF010000000
VERSION     DAOREF010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130189_length_1174_cov_3.592493"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..546)
                     /locus_tag="PKI33_15905"
     CDS             complement(70..546)
                     /locus_tag="PKI33_15905"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNM38556.1"
                     /translation="MSDKQKDSPAINKYIHPPIVAMFYIIVALLLGYLVPVLAGMSQT
                     LKNIGLGITFVGFLCGVGAFLEFRKARTTLDPHGSVKTVVTGGIYRFSRNPIYLGFLF
                     MVIGFPLAYGSLWGLVISPFFMVTLSRLVIEKEEAYLEKKFKEQYTSYKSKVRRWL"
     gene            complement(549..875)
                     /locus_tag="PKI33_15910"
     CDS             complement(549..875)
                     /locus_tag="PKI33_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38557.1"
                     /translation="MKTLTGVLEAYYEQGWEGMIVFSFMPNDGNGLNDLITLRDGQLL
                     TIYDQNEATLWSGKIQFVKRGFFDKHKLKADIWSDSKQKGVSYADWMDWFWRQPPLKA
                     TLEFKD"
     gene            complement(879..>1174)
                     /locus_tag="PKI33_15915"
     CDS             complement(879..>1174)
                     /locus_tag="PKI33_15915"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNM38558.1"
                     /translation="ANRARTKKLQPDDVKGGTFTLTNHGVSGSLFAFPVINQPQAGIL
                     GVGAMQKRVVVIDDAIAIRPMVYMSFVFDHRILDGASADWFLAKVKETLENWA"
BASE COUNT          316 a          278 c          265 g          315 t
ORIGIN      
        1 aatgattata agtacatttg ttctaaaata caagcaaaag aaaaaggcga ctttcgccgc
       61 ctttgggatt tacaaccaac gcctcacttt ggacttatag ctggtgtatt gctctttgaa
      121 ttttttctcc agataggctt cttctttctc tatgacaagc cgcgagagtg tgaccatgaa
      181 aaatggtgag atgacaagcc cccacaacga cccatatgca agcggaaagc cgatcaccat
      241 aaaaaggaag cccagataaa tggggttacg tgaaaaacga taaatgccac ccgtcaccac
      301 ggttttgacg gaaccatgtg ggtcgagcgt ggtgcgtgct tttcgaaatt caagaaacgc
      361 accaactcca cagagaaaac caacaaaggt gatacccagc ccgatatttt ttaaggtttg
      421 tgacatgccc gccaatacag ggacgagata tcctagtaat aatgcaacga tgatataaaa
      481 cattgccacg atgggtgggt ggatgtattt attgatcgcc gggctgtctt tttgtttatc
      541 gctcattttc aatccttaaa ttcaagtgtt gccttcaatg gcggctgacg ccagaaccaa
      601 tccatccaat cggcataaga gacgcccttt tgttttgagt ctgaccagat atctgctttg
      661 agtttgtgtt tgtcaaaaaa gccgcgcttt acgaactgga tttttccaga ccagagtgta
      721 gcctcattct gatcgtagat ggttagtagt tgaccgtctc gcaaggtgat gaggtcattc
      781 agcccatttc catcatttgg catgaaactg aagacaatca taccttccca gccttgctca
      841 taataggctt caagaactcc ggtcagcgtt ttcaattgtt atgcccaatt ctccaaggtc
      901 tctttgacct tcgccaggaa ccaatctgct gaagcgccat ccaagatgcg gtggtcgaag
      961 acgaaggaca tatacaccat cggtcggatc gcgatggcat cgtcaatcac gacaactcgt
     1021 ttttgcatcg cgcctactcc aagaattccc gcttgcggtt gattgatgac cgggaaggcg
     1081 aacagtgatc cgctcacgcc gtgattggtc aatgtgaatg tgccgccttt gacatcatcc
     1141 ggttggagtt tcttggtacg cgcacgattt gcaa
//
LOCUS       DAOREF010000949         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_130195_length_1174_cov_3.203753, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000949 DAOREF010000000
VERSION     DAOREF010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130195_length_1174_cov_3.203753"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1174)
                     /locus_tag="PKI33_15920"
     CDS             complement(<1..>1174)
                     /locus_tag="PKI33_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNM38559.1"
                     /translation="FDVPSGVNVTCGYAIVPENRTINSGRTIRLAVAVFHSRSSNPKP
                     DPVIFLQGGPGGEAVSLSANAYPILVDPFLSDRDYIAYDQRGTGLSEPALICDELERA
                     NRQDVYGTIESGSRELVYQNAFLSCAGLFQTQGIDVSAYNTVENAADLRDIVKLLGYE
                     KVNLYGASYGTRLALVAMRNHPEVIRSAILDSVMPVETNWVNEFPNIANYTLTNLFNS
                     CKADPDCNKAYPDIETVFWDLANKLDEKPVTLSTSAYPMGTVTETIDGAYLLSIIMGL
                     SKTTSYIDTAPQTIYRVRDGDYSTLIAAQYSLPYGFEGINTGLYISVMCHEHVLDTTP
                     EKLLEASTQLRVKDFVWSPFYGHLDKMFSACKTWGSTGPVLGEKDKVVSDIPSLVIT"
BASE COUNT          316 a          279 c          270 g          309 t
ORIGIN      
        1 cggtgatgac caagctagga atatcgctga cgactttatc tttctcgcca agcactggtc
       61 ctgttgatcc ccaagttttg caggcagaga acatcttatc cagatgccca taaaaaggag
      121 accacacaaa gtctttaact cttaactgag tagaggcttc cagtaatttt tcgggagtag
      181 tatctaaaac atgttcgtgg cacattactg agatatacag ccctgtatta atgccttcaa
      241 acccataggg cagggagtat tgagcggcaa ttaaggttga ataatctcca tcgcgtacgc
      301 gatagatcgt ttgaggggcg gtatcaatat agctagtggt cttggacaag cccatgatga
      361 tcgacaatag atatgctcca tcaatggtct cggttacggt tcccattggg taggcagagg
      421 tggataatgt tacaggtttt tcgtctaact tattggcaag atcccaaaat acagtttcaa
      481 tatctgggta ggctttgttg cagtcagggt cggctttaca ggaattgaat aagttggtta
      541 gtgtgtaatt ggcgatgttt ggaaattcat tgacccagtt cgtttccaca ggcataaccg
      601 aatctaaaat cgcgctccga atgacctctg ggtgattacg catggcaact aatgcaaggc
      661 gcgtcccata agacgcacca tataagttga ccttttcata ccctaatagt ttgacgatgt
      721 ctcttaagtc ggctgcgttt tcaaccgtat tgtaggcgct gacatcaatg ccttgcgtct
      781 ggaaaagccc ggcacaggaa agaaaagcat tttgatatac caattcacgc gaaccgcttt
      841 caatggttcc gtacacatcc tgtcgattcg ctcgttcaag ctcatcacag attaatgccg
      901 gttcagaaag ccctgtgccg cgttggtcat aagcgatgta atctcggtca ctcaaaaatg
      961 gatccaccaa gatcgggtag gcatttgcgc tcaacgaaac cgcttccccc cccggacctc
     1021 cctgtaaaaa gataaccggg tctggcttag gattcgaact tcgactatgg aatacagcaa
     1081 ccgccaaacg gatcgtccgc ccgctgttga tggtgcgatt ttcaggcaca atagcatacc
     1141 cacacgtcac atttaccccg gacggcacat cgaa
//
LOCUS       DAOREF010000950         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_130648_length_1172_cov_2.666070, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000950 DAOREF010000000
VERSION     DAOREF010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130648_length_1172_cov_2.666070"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKI33_15925"
     CDS             complement(<1..789)
                     /locus_tag="PKI33_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38560.1"
                     /translation="MAQRILRGLEITILLFVLAVLTGYSNPSLTNPIEKVRAYTRSLE
                     FDYVEWMANAAVLKMRAASINLPQTLNREEQKQVVMEYFKTTQFILEKEYLLSQLYAD
                     PSIQDKDAATETVRADLAELYKRQEQIAPLAEAILQDQVTQVLVEQGLTVGGQPIPNV
                     WYHSTPLPMALVISPRDHIEQTANISVNTDLTVDAQVKLEEDVSKGLNVSALVVQIGG
                     VGVYPTMVARTTNLNWLLSTIAHEWIHNYLTQRPLGLLYGESPEL"
     gene            complement(813..>1172)
                     /locus_tag="PKI33_15930"
     CDS             complement(813..>1172)
                     /locus_tag="PKI33_15930"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNM38561.1"
                     /translation="QMKKWMMPDIELSIQTDWEVGSPIFIRGTMSGKEFENKGLVLKF
                     EPEKNLQYSHLSSVSNLPDRAENYSILDFQLHSIHQKTQLMFTLRNFPTESIYKHMAF
                     YWNVTLEVFKRIAEEQR"
BASE COUNT          272 a          242 c          309 g          349 t
ORIGIN      
        1 caattcggga gattcgccat ataacaaccc aagtggacgt tgcgtgaggt agttatgaat
       61 ccattcgtgg gcgatggtgc tgagcagcca gttgagattg gtggtacgtg ccaccatagt
      121 gggatacacg cccacgccgc cgatctgcac cacaagcgct gaaacattca gccccttaga
      181 aacatcttct tcaagtttaa cttgtgcatc gaccgtcagg tctgtgttga ctgaaatatt
      241 ggcggtctgc tcgatatggt cgcgtggcga gatgacaagt gccatgggca ggggagtgga
      301 gtgataccag acatttggaa tgggttgccc gcccacggta agaccttgtt cgaccaagac
      361 ttgtgtgact tgatcttgca agatggcttc tgccaatggt gcgatttgtt cctgacgttt
      421 gtacagttcc gctaagtctg cacggacggt ttctgtagcg gcatctttgt cttgaatgga
      481 tgggtctgca taaagctgtg agagtaaata ttctttttca agaataaatt gggtcgtttt
      541 gaaatattcc attacgacct gtttttgctc ttcgcggttg agtgtttgcg gcaggtttat
      601 ggaagcggcg cgcattttta gcacggcggc atttgccatc cattccacat aatcaaattc
      661 taggctgcgt gtatatgcgc ggaccttttc gatcggattg gtgagggagg gattgctgta
      721 gccagtaagc acggcaagga caaagagcag gatcgtgatc tcgagcccgc gtaaaattcg
      781 ttgagccata gggtggatta tacccagttc gcttatctct gttcttcggc gattcttttg
      841 aacacctcaa gggtcacatt ccagtaaaac gccatatgtt tataaataga ctctgttgga
      901 aagttcctca gggtgaacat taattgtgtt ttttggtgga tagagtgtag ttggaagtct
      961 agaattgagt agttttctgc ccggtctgga agattggata ctgaactgag gtgactatat
     1021 tgaagatttt tctcaggctc aaattttagg acaagccctt tattttcgaa ctcttttccg
     1081 ctcattgtgc cgcgaatgaa aatagggctg ccgacttccc aatcggtttg gatggagagc
     1141 tcaatatctg gcatcatcca ttttttcatc tg
//
LOCUS       DAOREF010000951         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_132041_length_1166_cov_2.559856, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000951 DAOREF010000000
VERSION     DAOREF010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132041_length_1166_cov_2.559856"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKI33_15935"
     CDS             complement(<1..365)
                     /locus_tag="PKI33_15935"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNM38562.1"
                     /translation="MCGRFTLTVDPAQIEDVFSDYDFPNQFAPRFNIAPTQPVLAIPN
                     NAKNKADFFLWGLIPSWSKDPSIGNKLINARGETLAEKPSFRGSFKYKRCLIPVDGFY
                     EWKSHPGSTAKTPYFIHMK"
     gene            complement(376..>1166)
                     /locus_tag="PKI33_15940"
     CDS             complement(376..>1166)
                     /locus_tag="PKI33_15940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM38563.1"
                     /translation="LFAGYAGLGIENARLNMALQKELANSQGFIQELENKNTELERFT
                     YTVSHDLKSPLVTITGFLGYLELDARNGKFENFVRDMHRIRQAVDKMQTLLNDLLELS
                     RIGRIANDPMEVEFGVIVRDAMGLLDGAITAGKIQIDFVDEGQKVVGDRVRLLEVVQN
                     LIENSIKFMGKQTDPRIKIGSIHDEHNNRIFFVQDNGIGIEPQYQDRIFGLFNKLDAS
                     TEGTGIGLTLVKRIIEVHNGKIWLESEPGKGTTFYFTLPGIVKP"
BASE COUNT          302 a          257 c          265 g          342 t
ORIGIN      
        1 tctttcatgt ggatgaagta tggagtcttt gcggtgctac cagggtgaga tttccattca
       61 tagaatccat ctacaggaat cagacagcgt ttgtatttga agctgccgcg aaacgatggc
      121 ttttctgcca gagtctcacc acgcgcattg atgagtttgt tgcctatgct tgggtctttt
      181 gaccaagatg gaatcaagcc ccataaaaag aaatcggctt tgttttttgc attattggga
      241 atggcaagaa caggttgtgt gggcgcaata ttgaaacgcg gtgcaaattg attcgggaaa
      301 tcgtaatcgc tgaatacatc ttcgatttga gcagggtcaa cggtaagggt gaaacgtccg
      361 cacatgggta ctcctttatg gttttacaat gccgggtagt gtgaaataaa aagtagtgcc
      421 tttgcccggt tcagactcca gccagatttt tccgttatga acttcaataa tacgtttgac
      481 aagggtaaga ccgatgcctg tcccttcggt gctggcgtcg agcttattga acaagccaaa
      541 gatacggtct tgatattgtg gttcgatgcc gattccgtta tcttgtacaa aaaagatgcg
      601 attgttgtgt tcatcatgga ttgaaccgat cttgatacgg ggatcagtct gcttgcccat
      661 gaacttaatg gagttttcaa tcaggttttg aacaacctct aatagacgga ctcgatctcc
      721 aaccactttt tgcccttcat ctacaaagtc aatttggatt ttccctgccg tgatggcacc
      781 atctaacaat cccattgcat cgcggacaat gactccaaat tcaacttcca tcgggtcgtt
      841 ggcgatccgc ccgatgcgag aaagttcaag caagtcattc aacagggtct gcatcttatc
      901 cactgcctga cgaattcgat gcatatcgcg cacaaagttc tcaaatttgc cgttgcgcgc
      961 atctaactca agatagccaa gaaatcctgt gatggttact aaaggtgatt ttaggtcgtg
     1021 agatacggtg tacgtaaatc gttccaactc tgtattctta ttttctaact cttgaatgaa
     1081 gccttggctg ttcgccaatt ctttttggag tgccatattc aaacgggcat tttcaatacc
     1141 caagccggca tacccagcaa acagac
//
LOCUS       DAOREF010000952         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_132692_length_1163_cov_3.838448, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000952 DAOREF010000000
VERSION     DAOREF010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132692_length_1163_cov_3.838448"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..820)
                     /locus_tag="PKI33_15945"
     CDS             complement(29..820)
                     /locus_tag="PKI33_15945"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNM38564.1"
                     /translation="MQLFLGFLLALLIAFLAYKARSLNKSGAIAAAFTGTIIFGIGGL
                     NWAILLLIFFITSSALSRAFKNRKAKLEEKFSKGHERDAGQVFGNGGIATLFAALHFF
                     YPNEVWVWAAFAASLAAVNADTWATELGVLNPHPPRMITNLTKQVEKGTSGGISLVGT
                     LASLAGSALIAFFASLLTSNWSLFLLVTIAGLAGSLFDSFLGGTVQAMYFCPTDQKET
                     EKHPLHTCGTQTVHIRGWKWLDNDLVNFSCGAFGMLVSLLLLGIF"
     gene            complement(880..>1163)
                     /locus_tag="PKI33_15950"
     CDS             complement(880..>1163)
                     /locus_tag="PKI33_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38565.1"
                     /translation="IGLFVWASLIIPSTPQVSLAVQATGNAIETIPSSQLIIFPVASL
                     LLAVAGWIAGLYFYRWDRERVLAFIVWGSSTFTSLLFLLAVLFIVTTPV"
BASE COUNT          326 a          244 c          353 g          240 t
ORIGIN      
        1 gagaataggc ttggatgaaa ttgtcatgtt agaagattcc taagagaagt aatgagacca
       61 gcattccaaa cgcaccgcaa gagaaattga ctaagtcatt gtccagccac ttccagccac
      121 ggatgtgaac cgtctgtgtg ccacaagtgt ggagcggatg tttctcggtc tctttctggt
      181 cggtggggca gaagtacatg gcttgaactg tcccgccaag gaaggagtcg aagagagaac
      241 cagccaaccc ggcgatagtg accaagagaa acagtgacca gttcgaggtc aagagagaag
      301 cgaagaaggc gattaacgct gaaccggcta gagaggcgag tgtgcctacc agcgagattc
      361 cgccggaggt gcctttttcc acttgtttgg tgaggttggt gatcatgcgc ggcggatggg
      421 gattcagcac tccaagctcg gttgcccatg tatcggcatt gacggcggca agtgaagcgg
      481 cgaaggctgc ccatacccaa acttcattgg ggtaaaagaa atgtagtgca gcgaagagtg
      541 tggcgatgcc gccattgccg aagacttgtc ctgcatcacg ttcgtgcccc ttggaaaatt
      601 tttcttcaag cttggctttg cgatttttga aggcgcggct caaggctgaa gaggtgatga
      661 agaagatgag caagaggatc gcccaattca gaccgccgat gccgaagatg atggtgccgg
      721 tgaaggcagc ggcgatggcg ccgcttttgt tgaggctgcg ggctttgtat gcaagaaatg
      781 caatgaggag agcgaggaga aaaccgagga agagttgcat gatgagagtt ggaaagttag
      841 gatcggtttg aaggctttaa cttgcaaacc ttctaacttt tatacagggg tcgtaacaat
      901 gaacagaacg gcgagcaaaa acaataaact ggtgaaggtg ctggagcccc aaacaataaa
      961 agcgaggacg cgttcacggt cccaacgata gaagtaaaga ccagcaatcc aaccagcgac
     1021 ggcaagcaag aggcttgcta cgggaaaaat aattagctgc gaagagggaa tggtttctat
     1081 tgcgttgcca gtagcttgta ccgcaagcga cacctgtggc gtggatggga tgatgaggct
     1141 tgcccaaacg aatagaccga tat
//
LOCUS       DAOREF010000953         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_133940_length_1158_cov_2.454216, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000953 DAOREF010000000
VERSION     DAOREF010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133940_length_1158_cov_2.454216"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKI33_15955"
     CDS             <1..356
                     /locus_tag="PKI33_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNM38566.1"
                     /translation="SGGEAQRILLARALCQSTPILLLDEPTAHLDLQYQVSLLELIRE
                     LAHKENLAVLIALHDLNLAARYTDRVALLVGGRLNAIGTPREVLTPEKISNAYCLPVN
                     VVEHPYMDAPLVLPK"
     gene            complement(396..1157)
                     /locus_tag="PKI33_15960"
     CDS             complement(396..1157)
                     /locus_tag="PKI33_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNM38567.1"
                     /translation="MLTPLDYLFAALAALVAGAINALAGGGTLITFPVLTFLGIPAVS
                     ANITNTVALCPGYFGGTIAQAKDLKDQIKRLWMLIPASILGGILGGFLLLQTGEKLFK
                     DLVPYLILLACLLLAIQDPVRAWLTRRMAEGQGATLEQWTWLPVGLASVYGGYFGAGL
                     SVIVLSALGLTLDDSLTRLNALKQAVGFAVNVAAAVFFLFSGQVVWSVALVMAVGALI
                     GGNLGGRLAGKIKPSTLRWTVVTIGVIVAIIYFVR"
BASE COUNT          333 a          318 c          257 g          250 t
ORIGIN      
        1 tctctggcgg tgaagcgcaa cgcatcttac tcgcccgcgc cttatgccaa tctaccccca
       61 tcctcttact cgatgaacca actgcacatt tagaccttca atatcaagtc agtttattgg
      121 aattgattcg cgaattggca cataaagaaa acctcgctgt gttgatcgct ttacatgatc
      181 tgaatcttgc agcccgttat acagaccgag ttgctttatt ggttggtgga agactcaatg
      241 ccattggtac tccacgcgaa gtgctcaccc ccgaaaaaat ttcaaatgct tattgtctgc
      301 ctgtaaatgt tgtggagcac ccatatatgg atgctccact agtattacca aagtaagttt
      361 aatgtcattg caagagcacg gctcgcaatg acaaatcaac gcacaaaata aatgattgct
      421 acgatcacgc caatcgttac caccgtccac cgcaaggtgg acggtttgat tttgccagcc
      481 aaacgtccgc ccaaattgcc gccaataagc gctcccaccg ccataaccaa cgctaccgac
      541 caaactacct gacccgagaa taggaagaac actgccgccg ctacattaac cgcgaatccg
      601 accgcttgct tgagcgcgtt caaacgggtc agcgaatcat ccaaggtcaa gccaagagcc
      661 gagaggacga tcacgctcaa gcctgcacca aaatatccgc catagaccga ggcgagccca
      721 accggtaacc aagtccactg ctccagtgta gctccctgcc cttctgccat acgcctcgtc
      781 aaccatgcac ggacggggtc ttgaattgcc agcaaaagac aggcaagcaa aatgagataa
      841 ggcacaaggt ctttgaataa tttctcacca gtttgcaaaa gcaaaaatcc acccagaatg
      901 cctcccaaaa tactggcggg gataagcatc cacaggcgtt tgatttggtc tttcaaatct
      961 tttgcttggg ctatagtccc gccaaaatag ccaggacaga gcgccaccgt attcgtgata
     1021 ttcgcagaga ctgctggaat gccaaggaag gttaatacag ggaaggtaat aagtgtccca
     1081 ccgccagcga gggcgttaat cgctcctgca acaagggcgg caagagcggc aaagagataa
     1141 tcaagaggtg tgagcatg
//
LOCUS       DAOREF010000954         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_134127_length_1157_cov_4.186933, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000954 DAOREF010000000
VERSION     DAOREF010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134127_length_1157_cov_4.186933"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..808)
                     /locus_tag="PKI33_15965"
     CDS             complement(104..808)
                     /locus_tag="PKI33_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38568.1"
                     /translation="MNQTVEKAIAAYGGREFWLKAKRIEAEVSTHGLAFTIKSRPTFK
                     RAKISMEIARPFSKLMPIGKITQLAGVLDEHHVRLEDSTGRIVEERKDARKYFPGGLQ
                     RLFHWDDLDMAYFANYAFWNYFTLPVLLMREDIDWKEIGPGWLEATFPESIPTHSKIQ
                     RFRFDLKTGLLIQHDYTVDIMSKLATAANVVLEHKQGAGFLYPSRRLVTPRSPSGKPL
                     PAPTLIDITVHEFQVK"
     gene            complement(805..>1157)
                     /locus_tag="PKI33_15970"
     CDS             complement(805..>1157)
                     /locus_tag="PKI33_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNM38569.1"
                     /translation="KVLSKAAYGPKKKMPEEVRKHYYMPHEEKTARVGTWVFPREIIG
                     STPWLKTLWDQREKIQHIPTAIVWGDSDIAFKEGELKVWTSLMKNHTLNVLHRIGHYP
                     PEEAPEEVIKALKA"
BASE COUNT          259 a          278 c          293 g          327 t
ORIGIN      
        1 aggcaaaaaa ccgttgtaaa cacagtaacg cggggtgttt ccccgcgtta ctctataaat
       61 tccctgcaga attaatattt tatactgctc aaagctgcac ttgttatttg acttgaaact
      121 catgcactgt aatatcgatc aaagttgggg cggggagggg ctttccagat ggcgagcgcg
      181 gtgtgaccag tctccgcgag gggtataaga aaccagcgcc ttgtttgtgc tcgaggacga
      241 cgtttgcagc ggttgccagt ttgctcatga tgtctacggt atagtcatgt tgaatgagca
      301 gacccgtttt caggtcaaag cggaagcgct gaattttgct gtgggtgggg atgctttcgg
      361 ggaaggttgc ttcgagccag ccaggtccga tctctttcca gtcaatatcc tcgcgcatga
      421 gcaaaacagg cagggtgaaa taattccaga aggcatagtt ggcgaaatat gccatatcca
      481 gatcatccca atggaataac cgttgcagac cgccagggaa atacttgcgg gcatcttttc
      541 gttcttcaac gatcctgcca gttgaatctt cgaggcggac atgatgttca tccagcacac
      601 cagccagttg tgtgattttc ccaataggca tcagcttgga aaacgggcgg gcaatctcca
      661 ttgagatttt tgcgcgcttg aaggtgggtc tgctttttat cgtgaacgcc aacccatgtg
      721 tgctgacttc cgcttcgata cgttttgctt ttaaccaaaa ctctctgccg ccataagcgg
      781 caatcgcttt ttctacggtt tgattcatgc tttcaatgcc ttgatgactt cttcaggcgc
      841 ttcttcaggc gggtagtgac cgattctgtg caagacattg agcgtatgat ttttcatcag
      901 actggtccac acttttagct caccttcctt gaatgcaata tccgagtcgc cccatacaat
      961 ggcggtgggg atgtgctgaa tcttttcacg ttgatcccaa agcgtcttca gccacggtgt
     1021 ggagccgatg atctcacgcg gaaagaccca tgtgccaacg cgcgcggtct tttcttcatg
     1081 gggcatgtaa taatgtttgc gcacttcttc gggcatcttt ttcttcggtc catacgcggc
     1141 tttcgataag accttgc
//
LOCUS       DAOREF010000955         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_134418_length_1156_cov_3.275204, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000955 DAOREF010000000
VERSION     DAOREF010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134418_length_1156_cov_3.275204"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1156
                     /locus_tag="PKI33_15975"
     CDS             <1..>1156
                     /locus_tag="PKI33_15975"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNM38570.1"
                     /translation="ARRFILGKQGLWPGRRWRGLKGTDHAMHTMEYLQLDPLQIIARS
                     QDISLYSRVLNYKPEMWEDAAYKQRNFFDWGGWLAARPMEELPYWRVVMHRERDGGYG
                     DSRLRQMGKEHAKAIVEVRAILKDRGTVGNRDFEAADRKKTQSYRGRKDSALALYYLW
                     RIGDVMTHHRERFERVYALTENVAPQELIYEKTQEEADRFLVRKEISFGGITRIQRTG
                     DSYGRGEPDRAAKKLLEHMLADGEIIEVKVDGWKTIQYALAEDEKVISGLSAGRIPKA
                     WKPVDTTTTEEAVFLAPLDHVSARGRAKDLFGFDYVWEVYKPEHQRKFGYYVLPILWG
                     DRLVARFDSKLDRTTNTFIILGFWLEDKALTKDEAFAQALARGFSRFVNFL"
BASE COUNT          292 a          253 c          330 g          281 t
ORIGIN      
        1 tgcccgccgt tttattcttg gaaaacaagg cttgtggccc ggtcgtcgct ggcgcggact
       61 taaaggcact gaccatgcga tgcataccat ggagtattta cagcttgacc cgttgcagat
      121 cattgcacgc agtcaggata tttccttata tagccgtgtc ctcaactaca agccggaaat
      181 gtgggaagat gccgcctata aacagagaaa ttttttcgat tggggcggct ggctggcagc
      241 gcgcccaatg gaagagttgc cctattggcg ggtggtgatg catcgtgaac gagatggggg
      301 ctacggtgac tcacgtctgc gccagatggg aaaagaacat gctaaggcaa tcgttgaagt
      361 ccgcgctatt ttgaaagatc gaggaacggt aggcaatcgc gactttgagg cagcagaccg
      421 caagaagact caaagttatc gtggacgtaa agatagcgca ttagcgcttt attatttgtg
      481 gcgcatcggt gatgtaatga cgcatcatcg cgaacgattt gagcgggtct atgcactgac
      541 ggagaatgtc gccccgcagg aattgatcta cgaaaagact caagaggaag cagatcgttt
      601 tctggttaga aaagaaatca gctttggcgg catcacccgc attcaacgca ccggcgattc
      661 gtatggtcgc ggcgagcctg accgggcggc gaagaaactg cttgaacaca tgttggctga
      721 tggcgagatt atcgaagtaa aagtggatgg ctggaagaca atccagtatg cgttggcgga
      781 agatgagaag gtgatttcag gcttaagcgc ggggcgtatt ccaaaagcat ggaaacctgt
      841 agacacaaca acgactgaag aagctgtttt tcttgcgccg cttgatcatg tcagtgcgcg
      901 tgggcgtgcc aaagatttat ttggtttcga ttatgtgtgg gaagtataca agcctgaaca
      961 ccagcgcaag tttgggtatt atgtcttgcc gatcttatgg ggagaccgtc ttgtagcccg
     1021 cttcgacagc aagttggatc gaacgaccaa tacgtttatc attctgggtt tctggctcga
     1081 agataaagcc cttaccaaag atgaagcctt tgcccaagct ctggcgcgcg gattttcccg
     1141 ctttgtcaat tttctc
//
LOCUS       DAOREF010000956         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_135365_length_1152_cov_3.258888, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000956 DAOREF010000000
VERSION     DAOREF010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135365_length_1152_cov_3.258888"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKI33_15980"
     CDS             <1..348
                     /locus_tag="PKI33_15980"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNM38571.1"
                     /translation="LHDINIAWNQPSNRFGWSSYLGKECGSDSVPEYSVPARRENLAG
                     LPRAWVGVGDLDLFYTEDLFYAQRLQAAGVDCEVVVVPGAFHGFDAFTSQASIVQEFR
                     KSQIAALRKSFYG"
     gene            341..1018
                     /locus_tag="PKI33_15985"
     CDS             341..1018
                     /locus_tag="PKI33_15985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNM38572.1"
                     /translation="MDKSRYTLITFDVYTALFDIETSLTPIVHDTLKIEALSFVRAWR
                     SKQLEYALISNSLGATRIPFEALTRRSLDDTLFRHMLNPSEVIRTELVHAWHHLCPWQ
                     ESAKVVQSLHERGYTLGLLSNGDHALLDELSKTFPPVFEHIFSSEEAGYYKPHPSIYA
                     LPFQRLGIPATSLLHVAGSPTDVLGAKSARLQCVWSNRLGQPYLDVNHKADYELKDLS
                     GLLDILK"
BASE COUNT          290 a          277 c          274 g          311 t
ORIGIN      
        1 ttgcacgata ttaatatcgc ttggaatcaa ccgagcaatc gctttggctg gtcttcatat
       61 cttggcaaag aatgtggaag tgatagcgtg ccagagtatt ctgtgccagc tcgacgtgaa
      121 aatctcgcgg gtcttccgcg tgcctgggtt ggggtaggcg acttagacct tttctacact
      181 gaagatttgt tctacgctca acgcttgcaa gcagccggcg ttgattgcga agtagttgtg
      241 gtgccggggg catttcatgg ctttgatgca ttcacttcgc aagcttctat cgttcaggaa
      301 tttcgaaaat ctcaaatcgc ggcattgaga aaatctttct atggataaga gtaggtatac
      361 gttaatcacc ttcgacgtat acaccgcgct cttcgatatt gaaaccagcc tgacgccaat
      421 tgttcatgat actttgaaga tcgaggcgct gagctttgtc cgcgcatggc gcagcaagca
      481 attggaatat gcccttatca gcaattcact cggcgctacg cggattccct ttgaagcact
      541 cacacgccgc tcgttagatg acaccctctt tcgtcatatg cttaacccaa gcgaagttat
      601 tcgcacagaa ctcgtccatg catggcatca tttatgtcca tggcaagaat cagcaaaagt
      661 agtccagtct ctgcatgagc gcgggtacac ccttggtctg ctctcaaacg gcgaccatgc
      721 actattggat gaattgagca agacgtttcc tcccgttttt gagcatatct tctcgtcgga
      781 agaagcgggt tattacaagc cacatcccag catctatgcc ttgccatttc aacgcttggg
      841 cattccggca acatctctgt tgcatgttgc cggctcccca acagatgtgc tcggagcaaa
      901 atctgcacgc ttacagtgcg tttggtctaa tcgtctcggt caaccgtatt tggatgttaa
      961 tcacaaagct gattatgagt taaaagactt aagtggattg ttggatattt tgaaataagg
     1021 gtcaagaggt taaatcattg cggcggcgac gcgctaagat cgaataaaaa gccagcccga
     1081 atacaaagag agttgcgccc caagcagcat ttgctacaag aggaacacct gcttgtttaa
     1141 cagcgattcc ag
//
LOCUS       DAOREF010000957         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_136527_length_1147_cov_5.594322, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000957 DAOREF010000000
VERSION     DAOREF010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136527_length_1147_cov_5.594322"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKI33_15990"
     CDS             complement(<1..513)
                     /locus_tag="PKI33_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38573.1"
                     /translation="MKFVKPYRWMLLFAIALIFAQANLDLALPDYLSRIVNTGIQQGG
                     VEDSMPEAIRASEMDKVAIFLNAQDKEDILASYTLVTDSSPDYETYLEKYPTLADEPI
                     YVLNDISQSEIDRLDPAMAKALLTVSGIEKALKDPEAAAQMGGAFGGFDPSKLPAAQL
                     EQITSSVDEKF"
     gene            complement(610..1059)
                     /locus_tag="PKI33_15995"
     CDS             complement(610..1059)
                     /locus_tag="PKI33_15995"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNM38574.1"
                     /translation="MSDSVQFSQSLRQWVDIFMHHSMRGWHHFVKSTGLTMPQFSILM
                     QLHYKGRIGMSEVSEQFGVSAAAASQLVDKLVQAGFMERAEDPSDRRAKLIQLSPNGK
                     KLIETGISERYRWMDGLTSKLSADDKAKVVDALQVLTEAARQLDETA"
BASE COUNT          296 a          249 c          296 g          306 t
ORIGIN      
        1 gaatttttca tccactgagc tggtgatttg ctctaactgt gcagcaggaa gtttgctagg
       61 gtcgaaaccg ccgaaagctc cacccatttg ggcggcggct tctgggtctt tcaacgcctt
      121 ttcaatgcca gaaacggtca gcaaggcttt tgccattgcc gggtcgaggc gatcgatctc
      181 actctgcgaa atatcattta acacataaat gggttcatca gcgagagtag gatacttttc
      241 gaggtaggtc tcgtaatcgg gagaagaatc agttacgagt gtgtatgagg cgaggatgtc
      301 ttccttatcc tgtgcgttca agaagatagc gaccttgtcc atttcactgg cgcgaatggc
      361 ttcaggcatg gaatcttcca caccgccttg ttgaataccg gtgttgacga tgcgcgaaag
      421 ataatcgggc agggcaaggt cgaggtttgc ttgcgcaaag atcaatgcga tcgcaaacaa
      481 taacatccac cgataaggtt ttacgaattt cattaacttc atcatacgga ataaaactcc
      541 ttaatatcga aatatcacga tatgtttaac aaaagaatgt gtagaaaatt tgtagaagga
      601 agagagactt tatgcagttt cgtcgagttg acgtgccgcc tcagtcagga cttggagcgc
      661 atccaccact ttggctttgt catccgctga aagtttagat gtgagaccat ccatccaacg
      721 atagcgttct gagatgccgg tttcgatgag cttcttgccg ttgggggaga gctgaatcaa
      781 cttggcgcgg cggtcactgg ggtcttcggc acgttccata aagcctgcct gaacgagctt
      841 atccaccagt tggctggcag ccgctgcaga aacaccgaat tgctcgctca cctcagacat
      901 gccaatgcgt cctttgtagt gaagctgcat cagaatactg aattgaggca tggtcaaccc
      961 ggtggatttg acgaagtggt gccatccgcg catggagtgg tgcatgaaaa tatccaccca
     1021 ttggcgcaga gattgtgaga attggactga gtcactcata gttaagcctt ctttttagtt
     1081 aagcgggctt aactttatgc attagttaag ttttggtcaa gggatgagac gttagaaaat
     1141 tattaga
//
LOCUS       DAOREF010000958         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_137023_length_1145_cov_3.208257, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000958 DAOREF010000000
VERSION     DAOREF010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137023_length_1145_cov_3.208257"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKI33_16000"
     CDS             complement(<1..725)
                     /locus_tag="PKI33_16000"
                     /inference="COORDINATES: protein motif:HMM:NF025658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HNM38575.1"
                     /translation="MKIESRNNLYKELDHIWQRLIDAIKDQEPILLEGKERTFFLAYW
                     LYGEVGNGNFDQFYYNSTGDYSLEMVSALEEIGAYDAARILSECNRIFPNGVPPQNRD
                     ERIQALDALPSNSIAFLNRAGHELMDDSDFLLRIYHYYVTGEKISAAELRTVYYNFYA
                     GERFFQDLSKRPENEQFDFLLKKIKEMMMVPFSERYFLDKRIFEIRTYLLNLAYFDEK
                     AIVEIIERFIYKPGFNKQGSKAF"
     gene            complement(910..>1145)
                     /locus_tag="PKI33_16005"
     CDS             complement(910..>1145)
                     /locus_tag="PKI33_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38576.1"
                     /translation="NTLRALEEAIEEFAGVALVVSHDRWFLDRICTHIIVYEGDSVAK
                     MYIGNWSDYETAMMEKFGKDLTPHRVKYRTLKR"
BASE COUNT          308 a          254 c          231 g          352 t
ORIGIN      
        1 tcaaaagctt tgcttccttg cttgttaaat ccaggcttgt aaataaagcg ctcaataatt
       61 tcgacaatag ctttttcatc gaaatacgcc aaattcaaaa gataagttct tatttcaaaa
      121 attcttttat ctaaaaaata gcgttcacta aatggcacca tcatcatctc tttgattttc
      181 tttaagagaa aatcaaattg ctcattttct ggtctttttg ataagtcttg gaaaaagcgc
      241 tcaccagcat aaaaattata gtatacagtt ctgagttccg ctgctgatat tttttctcca
      301 gtcacataat agtgataaat tctcagcaaa aaatctgaat cgtccattag ctcatgacct
      361 gctctgttta agaatgcgat tgagtttgat ggcaaagcgt caagtgcttg aattcgttca
      421 tctctgtttt ggggcggcac cccatttggg aatatcctat tgcactcaga taaaattctc
      481 gccgcatcat acgcccctat ttcttccaaa gcggaaacca tttcaagtga ataatcgcct
      541 gtggagttgt aataaaactg gtcgaaatta ccattgccga cctccccata caaccaataa
      601 gccaggaaaa aagttctttc tttcccttcc aacagaattg gctcctgatc tttaatcgca
      661 tcgatgagtc tttgccaaat atgatccaat tctttataca ggttatttct agactcaatt
      721 ttcataagta gatccaagga tattgagtat ctatcgtttt agggtacggt atttcacccg
      781 atgcggaata aatccttgcc gaattttctt ccattatgat ttcataatcg atcgtagggg
      841 cgacgtctcg tcgcccaagg atgatggtgc aaacaaaaag gggcgggggg accccgcccc
      901 tacggacgtt taccgtttca gcgttctata tttcacccga tgcggagtta agtctttccc
      961 gaatttttcc atcatcgcgg tttcgtaatc ggaccagttg ccgatgtaca tcttggcgac
     1021 ggagtcgcct tcgtagacga tgatgtgggt gcagatgcgg tcgaggaacc agcggtcgtg
     1081 gctgacgact agggcgacgc ctgcaaattc ttcgatggct tcttcgaggg cgcgcagggt
     1141 gttga
//
LOCUS       DAOREF010000959         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_138018_length_1141_cov_3.315838, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000959 DAOREF010000000
VERSION     DAOREF010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138018_length_1141_cov_3.315838"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKI33_16010"
     CDS             <1..292
                     /locus_tag="PKI33_16010"
                     /inference="COORDINATES: protein motif:HMM:NF016098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNM38577.1"
                     /translation="KPLTQVDLANHAKMDVMMVSNVLRTLEDKGFVTRKPHPTDTRAK
                     ALSITGQGRKLATQTIGVVEKIDHDFFNSLGTDVKNFNKHLQALIITNRSTD"
     gene            352..>1141
                     /locus_tag="PKI33_16015"
     CDS             352..>1141
                     /locus_tag="PKI33_16015"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNM38578.1"
                     /translation="MSTPVIVDAIRTPIGALGGILSSVRPDDMAAHVIKSILERNQID
                     PAKIEEVFMGCANQAGEDNRNVARMAALLAGLPVEVGGVTVNRLCASGLNAINMASRA
                     IKAGEGDVYIAGGVESMSRAPYSLPKAEAGFSFGNLTAYDTALGWRYPNPKMKAMHGT
                     DSMGETAENIAAERPHITREKQDAFAMRSHQRAIAAIDSGRFKQEIVPVTIPQKKGDP
                     KVIDTDERPRRDTTAESLGKLKAAFRDGGTVTAGNSSGLNDGASA"
BASE COUNT          302 a          266 c          301 g          272 t
ORIGIN      
        1 taaacctctt acacaggtgg atcttgccaa tcatgccaaa atggatgtga tgatggtgtc
       61 taacgtgcta cgaacgctgg aagacaaagg tttcgttact cgtaaaccgc acccgactga
      121 tacccgagca aaagcattgt ccattaccgg acagggacgt aagttggcta ctcaaactat
      181 tggcgtggtt gagaaaattg accatgattt tttcaacagc ttgggaacag atgttaagaa
      241 tttcaataaa catctgcaag cgttgattat tacaaaccgt tctacagatt aacaagaact
      301 gctctctctg gtgttagaat caatttatga attcaaactc tggagggacg catgtccaca
      361 cctgtaattg ttgatgccat ccgcacgccg atcggcgcgt tgggcggcat tctttcatct
      421 gtgcgcccag acgatatggc ggcacatgtg atcaaatcca ttcttgaacg aaatcaaatt
      481 gaccctgcga aaatcgaaga ggtctttatg gggtgtgcga accaagctgg cgaagataat
      541 cgtaatgtgg cgcgtatggc ggcattactt gccggtctgc ccgtggaagt gggtggtgtg
      601 accgtcaacc gcttgtgtgc ttcaggttta aacgccatca atatggcgtc gcgagccata
      661 aaagctggtg aaggggatgt ttacatcgca ggtggcgtgg agtctatgag ccgtgcaccg
      721 tattcacttc ccaaagccga ggcggggttt tccttcggca atctcactgc atatgacacg
      781 gctctaggct ggcgttaccc aaatcccaaa atgaaggcga tgcacggaac tgactcgatg
      841 ggtgagaccg cagagaatat tgctgcggaa cgtccacaca tcacacgcga aaaacaggat
      901 gcctttgcga tgcgaagcca tcaacgcgct attgccgcga ttgactcagg gcgcttcaaa
      961 caggaaattg tgcctgttac tattccccag aaaaaaggtg acccgaaagt gattgacacg
     1021 gacgaacgtc cgcgccgcga tacgacggcg gaaagcctgg gtaaactcaa agctgctttt
     1081 cgtgatgggg gaactgtcac ggctgggaat tcgtctggat taaatgatgg cgcttctgca
     1141 t
//
LOCUS       DAOREF010000960         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_140563_length_1131_cov_4.393123, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000960 DAOREF010000000
VERSION     DAOREF010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140563_length_1131_cov_4.393123"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PKI33_16020"
     CDS             <1..264
                     /locus_tag="PKI33_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38579.1"
                     /translation="MFAAIMLPLAGRALISGAILTWTRALGEFGATILFAGNLEGITQ
                     TMPMAIYLGFERNVGVALSLSVVLIFVSTLLLLLTRKLEKEKD"
     gene            complement(362..583)
                     /locus_tag="PKI33_16025"
     CDS             complement(362..583)
                     /locus_tag="PKI33_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38580.1"
                     /translation="MAKKVWQVEKTKYCEHLQQAIAIEVEVVYPAEQLPEQTPRVMAR
                     RCSKAIECNCVEHPSCSLSGTNPDLDPVE"
     gene            complement(694..>1131)
                     /locus_tag="PKI33_16030"
     CDS             complement(694..>1131)
                     /locus_tag="PKI33_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNM38581.1"
                     /translation="ISFDRATGDLWIGDVGQNQYEEIDYLPAGSPGGANFGWSVMEGL
                     HGYDGEAQPGLLLPVVEYSHEIGCSVTGGYVYRGSMPEWNGVYLYGDYCSGSVSGLIL
                     VDGQWQSRVLFEAVATITSFGQDEAGELYFTSDNGSIYILAKK"
BASE COUNT          272 a          304 c          272 g          283 t
ORIGIN      
        1 atgtttgccg ccatcatgct gcctctggct ggacgtgccc tcatcagcgg agctattctc
       61 acctggacgc gcgcgcttgg cgaatttggg gcaaccattt tatttgccgg gaatttagag
      121 ggcatcacgc aaaccatgcc catggcaatt taccttggct ttgaacgaaa tgtaggggtt
      181 gcactatcgc tttcagtggt gcttattttt gtttccacct tattgctatt gctcacaaga
      241 aaactagaaa aagaaaagga ctaactcttt taacggctta cgccgcgcgt tgcgcggcgt
      301 agccttaaga ccaactcaga gggaggagag agagtttgga tgaacagcgt tgtgcgctgc
      361 tctattcaac agggtcaagg tcaggatttg tcccgcttag ggagcaagag gggtgttcta
      421 cgcaattgca ttcgattgct tttgaacagc gccttgccat aacacgggga gtttgctcgg
      481 gcagttgctc agccgggtat acaacctcga cttcgatggc gattgcttgc tgcaagtgct
      541 cgcaatattt ggttttttca acttgccaaa ccttttttgc cataactgct cctgactcag
      601 atgtccctag tttagaacca aaacgcctgt cagaccagtg acaggcgtca taaactcgga
      661 tgacaaagca cctattatgg atgtgataag tctttatttc tttgcaagga tgtaaatact
      721 gccgttatcg ctggtgaagt acaactcgcc agcctcgtct tgcccaaaag aggtgatggt
      781 tgccaccgcc tcaaacagga cccgagactg ccactgccca tcgaccagaa tcaaccctga
      841 aacagagcca gaacaatagt cgccgtacaa gtacacgccg ttccattctg gcatggagcc
      901 gcgatagaca tatccgcctg tcacagagca gccaatttca tggctgtatt cgaccaccgg
      961 taacagtagc cctggctggg cttccccgtc gtacccatgc agcccttcca ttacactcca
     1021 gccaaagttg gcaccgcctg gcgagccggc tggcaaatag tcgatctctt catattggtt
     1081 ctgccccaca tcgccaatcc acagatcgcc cgtagcacgg tcaaatgaaa t
//
LOCUS       DAOREF010000961         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_143395_length_1120_cov_4.794366, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000961 DAOREF010000000
VERSION     DAOREF010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143395_length_1120_cov_4.794366"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKI33_16035"
     CDS             <1..773
                     /locus_tag="PKI33_16035"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNM38582.1"
                     /translation="EEVCVLTATRFGMIKKSLVTELPGPSSQTFVLVKVNEGDKLNSV
                     GITDGKKDILLVTASGMAIRFKEEDVRPMGLVAAGVNGMKLEDKDEVVGMEILPADGD
                     IFLLTNEGKAKRVDQKDFPVQGRYGKGVVAWDMPGKSTLAAVASGKPNHVATVHMSKG
                     APKSTRLDAAGLRKRAAGKGDVVIEVKPGESITSIAVGWALESYVKVAKAEEPKSKKE
                     GKPKAAKAPAKKAAKPVAKAKGKPAKKAPAKAKPKKKK"
     gene            849..>1120
                     /locus_tag="PKI33_16040"
     CDS             849..>1120
                     /locus_tag="PKI33_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38583.1"
                     /translation="MEYTIQLDQANGLILVHAAGDWESDKDNLMVQQIMQTVTESGLR
                     KILLDIRELKFDLSMAHIFERAVAMRDARMKQDKVSSRVALVYDAQ"
BASE COUNT          329 a          220 c          331 g          240 t
ORIGIN      
        1 gtgaggaagt atgtgtgtta accgctacac gcttcggcat gatcaagaag agtctggtca
       61 cggaattgcc gggcccatcg tcgcaaacct ttgtgttggt gaaggtcaat gaaggcgata
      121 agctcaattc tgttggtatc acggacggaa agaaagatat cttgctcgtc actgccagcg
      181 gcatggcgat ccgcttcaag gaagaggatg tgcgcccgat gggacttgtg gcggcaggcg
      241 tgaacggtat gaagctcgaa gataaggatg aagtggtcgg tatggaaatt ttgcctgctg
      301 atggcgatat cttcttgttg accaacgaag gcaaagccaa acgtgtggac cagaaagact
      361 tccccgtgca gggacgttat ggcaagggcg tagtggcttg ggatatgccg ggtaaatcga
      421 ccttggcggc agtggcttct gggaagccga accatgttgc tacggttcac atgagcaaag
      481 gcgcgccgaa atccacacgc ttggatgcgg ctggtctgcg taaacgcgcc gctggcaagg
      541 gcgatgtagt gatcgaagtc aagccgggtg agagcatcac ctccattgca gtaggatggg
      601 ctttggagag ttatgtgaag gtggcaaaag cagaagagcc aaagagcaag aaagaaggaa
      661 agccgaaagc ggcaaaggca cctgcgaaaa aagcggctaa acctgttgca aaggcaaaag
      721 gaaaaccggc aaagaaagct cctgcaaagg ctaaacctaa aaagaagaaa taatagataa
      781 aactcccatg aagtgcttca tgggagtttt gttgtggaac tagttataat cgtagcaagg
      841 agaatatcat ggaatacacc attcaattgg atcaagccaa tggattgatc ttggtccatg
      901 ctgcggggga ttgggagagc gataaggaca atttgatggt tcaacagatt atgcagacag
      961 tcaccgaaag cggattgcgt aaaatattac tcgatattcg agaactgaaa tttgacctat
     1021 ccatggcgca tatttttgaa cgagctgttg ccatgcgtga tgctcgcatg aaacaagaca
     1081 aggtcagctc tagggtggca ttggtctatg atgcacaaaa
//
LOCUS       DAOREF010000962         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_143484_length_1120_cov_2.295775, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000962 DAOREF010000000
VERSION     DAOREF010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143484_length_1120_cov_2.295775"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..>1120
                     /locus_tag="PKI33_16045"
     CDS             232..>1120
                     /locus_tag="PKI33_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38584.1"
                     /translation="MPSLPSPTSFTECNPSSYTANIYRPTPLPTFPVTVASTSIPNIV
                     STAQPIPVDIHLHFQKLVQEVSRWTAITPFPLGDSSEVWVAITFLSPELLRTVSTTDL
                     LSENPASINLDIQVNTMLAEIAKREKLLFFLTAFKAHTGGEATNSHTFQIDVENLKLI
                     NAKNLDIKPAYYDHNLGQKMEFPAPGESSQLAQSLISNYGYLYYPLGVESDGTCVEVL
                     ESTFNTKIILEASSIIMDGKSTGPYTWTIDYTYLLNTTSNPVPNATFIVNSAIESTDA
                     LPTVPVISDAFWEEYAKFIW"
BASE COUNT          308 a          283 c          215 g          314 t
ORIGIN      
        1 tctggtcaac atgccccccc catgctgaca ggcggcggta cctttttaac aatgctaaaa
       61 tacacttgaa gaagctccga ggggtcaata taatgttttc tatctgatca attcaatttg
      121 gaggcgggct tgatggtttt aaatgatgtc gtagtacaac gcttttatgt gtcttgggta
      181 cggtggttta tagggtttgt aatcataacg gggattttcg catgcagcta catgccttcc
      241 ttaccatccc ccacctcctt tacagaatgt aacccttcat cctacacagc aaatatctat
      301 cgtccaacgc cgttgcctac gttccctgtt acagtagcat ccacatcgat cccaaatatt
      361 gtttcgaccg ctcagcccat cccagtggat atccatctgc attttcaaaa gttagttcaa
      421 gaagtcagcc gctggacggc gattactcct ttcccgcttg gcgattcaag cgaagtatgg
      481 gtggcgatca catttctaag ccctgaactt ttacggactg tttccactac agacctctta
      541 agtgaaaacc cagccagtat aaatttagat attcaagtta atactatgct cgcagagatt
      601 gccaagcgcg agaaactatt atttttctta acagccttca aggcacatac aggcggcgaa
      661 gccacaaact cacatacgtt tcaaattgat gtagaaaatc taaagcttat caatgccaaa
      721 aacctcgata tcaaacctgc atactatgac cataaccttg ggcaaaaaat ggaatttccc
      781 gcgccagggg aatcttctca gctcgcacaa tcgttaatat ctaattatgg atatttgtat
      841 tatccattag gcgtagaatc tgatggcacc tgtgtggagg tccttgaatc gaccttcaac
      901 accaagatca tccttgaggc atcctccatt atcatggatg gaaagtctac gggaccgtat
      961 acgtggacaa tcgactacac ctatctctta aacacaacca gcaaccctgt ccccaatgca
     1021 accttcattg tcaattccgc tattgagtcg acggacgcct tgcctactgt tcccgtcatc
     1081 tcagatgcgt tctgggaaga atacgcaaaa tttatttggg
//
LOCUS       DAOREF010000963         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_145233_length_1113_cov_3.300567, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000963 DAOREF010000000
VERSION     DAOREF010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145233_length_1113_cov_3.300567"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKI33_16050"
     CDS             <1..224
                     /locus_tag="PKI33_16050"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38585.1"
                     /translation="IGVTRQTAVLAYIFGDGFSNLAYPTNPVLLISLGLTVVSYPKWL
                     RWTAKLWLWVILATVTFLGIAVAIHFGPF"
     gene            complement(268..>1113)
                     /locus_tag="PKI33_16055"
     CDS             complement(268..>1113)
                     /locus_tag="PKI33_16055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNM38586.1"
                     /translation="INVSVNVPPDAQTLVLGDAGRIRQVLSNLLSNAIKFTERGGAVF
                     VTLSSSQIKDVMMTTFTVQDTGLGIPDAVKPKLFNPFTQADDSQTRRYGGSGLGLAIS
                     KRLIDLMHGEIGFETLEGKGSTFWFSLPLTMINETTEVVAEKQMPRQKDPLPFVVTKP
                     ALIVEDNLLNRDLISLQLRELGLNASYATNGKEAVELLKFQPDEYSIVLMDMNMPVMD
                     GPAATKLIRENEAGTTRHVPIIAVTANVMTGTREFCLQAGMDDYLSKPITLQSMREVL
                     EKWLV"
BASE COUNT          293 a          273 c          224 g          323 t
ORIGIN      
        1 tgatcggtgt cacccgtcag actgcggttt tggcatatat ctttggcgat ggcttctcaa
       61 accttgccta tcccaccaac ccggtcttgc tcattagtct tggtctcacc gtagtaagtt
      121 accccaaatg gctacgttgg acagccaaac tgtggctctg ggtaattcta gcaactgtga
      181 catttttagg gattgctgtt gcaattcatt tcggtccgtt ctaaagcata aaaagagcct
      241 gcacaaaatg tgcaggctct ttttatatca aacaagccat ttttccaaga cttcacgcat
      301 agactgtaac gtgatcggtt tgctcaggta atcatccata ccagcttgca aacaaaattc
      361 acgggtgcct gtcatcacat ttgcagtaac agcaataatt ggtacatgcc ttgttgtccc
      421 cgcctcattc tcgcggatca actttgtagc agccggtcca tccataacag gcatattcat
      481 atccatcaga acgatagaat attcatcggg ctggaatttc aataactcga ccgcttcctt
      541 tccattggta gcataactgg cattcaagcc taattctcgt aattgaagtg aaatcaagtc
      601 tcgattaagc aagttatctt ctacaatcag cgctggtttc gtcacgacaa atggcaaagg
      661 gtctttttgc cgaggcatct gcttttctgc aaccacttcg gtcgtttcat ttatcatggt
      721 taatggcaag ctaaaccaga aggtggaacc cttcccttcc aatgtctcaa acccgatttc
      781 tccatgcatg agatcgatca agcgttttga aattgccaac cccaagccag accctccata
      841 gcgccgcgtc tgagaatcat ctgcttgagt aaatggatta aataatttcg gcttgactgc
      901 atcagggatg cccaaccctg tatcttggac tgtaaaagta gtcatcatca catctttgat
      961 ttgtgagctt gagagtgtga caaaaacagc gcctccacgt tctgtgaatt tgatagcgtt
     1021 actcaagagg ttgctcaata cctggcggat tcttccagca tctcctaaga ctagagtttg
     1081 agcatcaggt ggaacattaa ccgaaacatt gat
//
LOCUS       DAOREF010000964         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_149064_length_1099_cov_4.428161, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000964 DAOREF010000000
VERSION     DAOREF010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149064_length_1099_cov_4.428161"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1099
                     /locus_tag="PKI33_16060"
     CDS             <1..>1099
                     /locus_tag="PKI33_16060"
                     /inference="COORDINATES: protein motif:HMM:NF018444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38587.1"
                     /translation="GVGKSFAYLVPAALFAHQNNTRVVVSTNTINLQDQLIKKDIPDL
                     QAALNLNVRAAVLKGRSNYLCPRRFQYLRSHGPSNATEMRVLAKLIVWQLSNPSGDRN
                     DLNLQGPLEREVWSRLSAEDDNCTTEACLGRMGGTCPFYRAKQAAQNSHILIVNHALL
                     LSDVSTGSKVLPEYDYLIVDEAHHMETAVTNALSFRMTQGDLERMLKELGGSSAGLLG
                     TILTDTHDTLRPSDFGLFQQKAKRATDQAFRVEQLSKEFFSYLGEFIKAQREGQPQSN
                     YSWQMRITPAARTLQGWDDLEMVWGQISETMANLLKTLDEIYKSLGELYNDGHDEVED
                     VMGSLGTLIRRMTEAETAASGLMHNPTNDLIY"
BASE COUNT          283 a          297 c          267 g          252 t
ORIGIN      
        1 ggtgtgggta aatcctttgc ctatcttgtg ccagccgcgt tatttgccca tcagaacaac
       61 acgcgcgtcg ttgtatccac taacacgatc aacctgcaag accaactcat caaaaaagat
      121 atcccagacc ttcaagctgc gttgaactta aatgtgcgtg ccgctgtgtt aaagggacgc
      181 tcaaactacc tttgcccacg tcgctttcaa tacctgcgct cgcacggacc tagcaatgcc
      241 accgaaatgc gtgtgttggc aaaactcatc gtttggcagt tgagcaatcc cagcggtgac
      301 cgaaacgact tgaatttgca aggacccctt gaacgcgaag tgtggagccg cctctctgca
      361 gaagatgata actgcaccac cgaagcctgc cttggcagaa tgggcggaac gtgtcctttt
      421 tatcgcgcca aacaagccgc gcaaaattca catattctca ttgtcaacca tgccttgctt
      481 ctttcggatg tttcgaccgg cagcaaagta ttacccgaat acgattacct gatcgtggac
      541 gaagcgcatc acatggaaac cgctgtgacc aatgcacttt ctttccgtat gacccaaggc
      601 gacttggaac gtatgttgaa agaattgggt ggttcatcgg cggggttgct cggcacaatt
      661 ctgacggata cgcatgatac cttgcgcccg tctgattttg gtttattcca acaaaaggca
      721 aagcgcgcaa ccgaccaagc cttccgcgtg gagcaacttt ccaaagaatt cttttcatat
      781 cttggcgagt tcataaaagc ccagcgtgaa ggacagccgc aaagcaatta ctcttggcaa
      841 atgcgcatta cacccgccgc gcggaccctg caaggctggg acgatctcga aatggtttgg
      901 gggcagatta gcgagacgat ggcaaacttg ctcaaaacgc tggatgagat ttacaaatct
      961 ctcggcgagt tatacaacga tggtcatgat gaagtggaag atgtgatggg cagccttggc
     1021 acgctcatcc gccgcatgac cgaggcggaa accgccgcct ctggcttgat gcacaacccc
     1081 accaacgact tgatctact
//
LOCUS       DAOREF010000965         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_149084_length_1099_cov_3.277778, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000965 DAOREF010000000
VERSION     DAOREF010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149084_length_1099_cov_3.277778"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKI33_16065"
     CDS             <1..227
                     /locus_tag="PKI33_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38588.1"
                     /translation="MSDIGHLMQVINRLVDTGNTVVVIEHNLHVIKNADWIIDMGPEG
                     GHKGGQVMFEGTPKELLKAKQSLTSAYLRN"
     gene            262..753
                     /locus_tag="PKI33_16070"
     CDS             262..753
                     /locus_tag="PKI33_16070"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNM38589.1"
                     /translation="MNIEPKLENRKEQPYVAVRVQVGIPFGEVLGGFWGEAGAYLASK
                     GMKPSGPPFIRYLTTDMDKKLDIEVGFPVDNIVDGNDRITTGVFPAGQYAVYLYFGHY
                     ENLVAVTAEFLAWAEKNNITWQTTKKDEVEWWVGRIEWYPTDPDNEPDPQKWQTELAF
                     LVK"
     gene            766..>1099
                     /locus_tag="PKI33_16075"
     CDS             766..>1099
                     /locus_tag="PKI33_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38590.1"
                     /translation="MKQGDPNNNNGFPKIGAPAMRALEAAGYTNLKQLTKVTEVELAQ
                     LHGMGPKALGILRETLKAQGLSFKQGKAGVTLKKKGSPVSRTDKVDEFLENLSHPLTA
                     EVEAVRSII"
BASE COUNT          318 a          231 c          298 g          252 t
ORIGIN      
        1 acatgtcgga tattggtcat ctgatgcaag tcatcaatcg ccttgtcgat acgggcaata
       61 ccgtcgtagt catcgaacac aatttgcatg tcatcaagaa cgccgattgg atcatcgaca
      121 tgggacccga aggtggtcac aaaggcgggc aggtcatgtt cgaaggcaca cccaaagagt
      181 tattgaaagc gaagcagtcg cttaccagcg cctatctccg caactagcag acaaatgatt
      241 tcggtttaag gagagtgagt catgaatata gaaccaaaat tggaaaatcg taaagaacaa
      301 ccatatgtag cagtccgcgt acaagtaggg ataccctttg gtgaggtgct tggaggcttc
      361 tggggtgaag cgggtgccta tttagccagc aagggaatga aaccttccgg tccgcctttt
      421 attcgttatc tcactactga tatggataaa aaactcgata tagaagtcgg ctttcctgtt
      481 gacaacattg tcgatggaaa tgaccgcatc acaacaggcg tatttcccgc tggacaatat
      541 gctgtgtact tgtattttgg acattacgaa aatcttgtgg ctgtgaccgc tgaatttctg
      601 gcttgggccg aaaaaaataa tattacctgg caaactacga aaaaagatga ggttgaatgg
      661 tgggtgggac gaatcgaatg gtacccaact gacccagata atgagccaga tccgcaaaag
      721 tggcagacgg aacttgcgtt tcttgtgaaa taagcaggag tttgtatgaa acaaggtgat
      781 ccaaataata acaatggttt tccaaagatc ggagcgcccg ccatgcgcgc cttggaggcg
      841 gcgggataca caaaccttaa gcagttgacc aaagttacgg aagtggaact cgctcaactg
      901 catgggatgg gtccgaaagc gttagggatt ttgcgcgaga cgttgaaggc gcagggcttg
      961 tcttttaaac aaggaaaggc aggcgtaacg ctgaagaaga aaggctcgcc cgtcagccgc
     1021 acggataagg tggatgaatt ccttgagaat ctcagtcacc cgcttacggc ggaggtggag
     1081 gcggtgcggt ccatcatca
//
LOCUS       DAOREF010000966         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_149445_length_1098_cov_2.245446, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000966 DAOREF010000000
VERSION     DAOREF010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149445_length_1098_cov_2.245446"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PKI33_16080"
     CDS             <1..800
                     /locus_tag="PKI33_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNM38591.1"
                     /translation="DFTTINTPKVMGGITHACGEVATAWYEGAIKTVHHVSTAEAAEM
                     SKLLENTFRMINIGLVNELAIMCEYLGVDVWEVIDAAATKPFGFMKFTPGPGLGGHCI
                     PIDPLYLSWKMRSFNYNARFIELASEINTNMPRYVVSRVLEAMNDLGKTLKGSKVLVL
                     GVAYKPDIDDVRESPALDVIGLLRKKGALVEYHDPYIPHIHHEYDGWRMDSVKDMMKS
                     VKEADVVVVVTNHKVYDYKAVIESAKFVFDSRNATGKLGKFANVERL"
     gene            865..>1098
                     /locus_tag="PKI33_16085"
     CDS             865..>1098
                     /locus_tag="PKI33_16085"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNM38592.1"
                     /translation="MAHYLVTGAAGFIGARTSEILIGQGHTVVGIDNVNDAYDPRMKE
                     YRLKKLMAMPGFKFHRRDISDKSCIELFKDEKLD"
BASE COUNT          297 a          214 c          288 g          299 t
ORIGIN      
        1 aagatttcac gaccatcaac acaccgaaag tgatgggtgg tattacgcat gcatgcggcg
       61 aagttgctac ggcgtggtat gaaggtgcga taaagactgt ccaccatgtt tccacagcag
      121 aggctgcaga aatgtcgaaa ctattagaaa acaccttccg catgatcaac attggtttgg
      181 tcaatgaact ggcgatcatg tgcgaatatc ttggcgtgga tgtctgggaa gtgatcgatg
      241 ccgcagcaac caaaccattc ggattcatga aatttacacc gggtccgggc ttgggcggtc
      301 actgcatccc gattgacccg ctttatcttt cgtggaaaat gcgctcgttt aattacaacg
      361 cccgctttat tgagttggcg tccgagatca ataccaacat gccgcgctac gtcgtcagcc
      421 gtgtgttaga agcgatgaat gatcttggca agactttgaa aggctcgaag gtgttggtct
      481 tgggtgtggc gtacaagccg gatattgacg atgtgcgaga atcccctgct ttggatgtaa
      541 ttggtttact gcgtaagaag ggcgcgttgg tcgaatacca tgacccatat attccacata
      601 tccatcacga atatgatggc tggcgaatgg atagcgtcaa agatatgatg aaatctgtga
      661 aagaagctga tgttgtggtc gtcgtgacga accataaagt ttatgattac aaggcggtga
      721 ttgagtctgc gaaatttgta ttcgattcgc gcaatgcaac cggtaaactt ggtaagtttg
      781 caaacgtgga acgactttaa ttctttacgc attatgtatt cttaaataca taatgcgtta
      841 ttgtttaact tgtaaaggag aaacatggct cattatctgg ttacgggagc agcaggtttc
      901 attggtgcaa ggacatcaga gattctgatc gggcagggac atacggttgt aggcattgat
      961 aacgtcaacg atgcgtatga tccgcgcatg aaggagtatc gtcttaagaa gttgatggca
     1021 atgccgggat tcaaatttca ccgtcgcgat atttctgata aatcctgcat cgagttgttc
     1081 aaggatgaaa agcttgat
//
LOCUS       DAOREF010000967         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_149749_length_1097_cov_2.114203, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000967 DAOREF010000000
VERSION     DAOREF010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149749_length_1097_cov_2.114203"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKI33_16090"
     CDS             <1..933
                     /locus_tag="PKI33_16090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNM38593.1"
                     /translation="NALQKQTYRDFEIIIVDNGSNQFDALSQIQKKHSNLSILIERSK
                     NNSGYAAGNNLGVKRACGHWLALLNDDAFPQSDWLEMLVHAAEQYKDYTFFASRQIQY
                     NHPEFLDGAGDAYHITGLAWRRYYNQPAENYGLLNEEVFSACPAAALYSREEFLKIGG
                     FDEDYFSYFEDVDLGFRLRLNGAKCLYVPDAVVHHVGSASTGKRSDFSVYYGYRNMIW
                     TFFKNMPFPYFWIFLPLHIATVIFFTIYLTWRGQGSAIWLAIFDALTTLPNMLKKRKM
                     IQKNIHIKTRSLYKIMSTGLIEPFHEFMRRNRVK"
BASE COUNT          313 a          238 c          208 g          338 t
ORIGIN      
        1 aatgcacttc aaaaacagac ctatcgagat tttgaaatca taattgttga taatgggtct
       61 aatcagtttg atgctttaag ccaaattcaa aaaaaacatt ccaacctatc tattctaatt
      121 gaaaggtcca agaataattc tggttacgca gctgggaata atttgggtgt caaacgtgct
      181 tgcggacatt ggcttgctct cttaaatgat gatgctttcc cacaatctga ctggcttgag
      241 atgcttgtgc acgcggcaga gcaatataaa gactacacat tttttgcttc tcgccaaatt
      301 caatacaacc accccgaatt tctagatggt gcaggggatg cttaccatat taccgggcta
      361 gcatggcggc gatattacaa tcaacctgct gagaactatg gacttttgaa cgaagaagtt
      421 tttagcgcct gccctgccgc cgctttatat tctcgtgaag aattcctaaa gatcggcggt
      481 tttgatgagg actatttctc ttactttgaa gatgtagatc ttggttttcg cctgcgtttg
      541 aatggtgcta aatgtttgta tgtacctgat gcagttgttc atcatgttgg ctctgccagt
      601 accgggaaac gtagcgactt ctcggtgtat tatggctacc gcaatatgat ctggacattt
      661 ttcaagaata tgccatttcc ttacttttgg atatttttgc ctttacatat cgcaaccgtt
      721 atctttttta caatttatct tacctggcga ggtcagggct ccgcaatctg gctcgcaatc
      781 tttgatgcac tgactacact tcccaatatg ctaaaaaaaa gaaagatgat ccaaaaaaat
      841 atccatataa agactcggag tctttataaa attatgtcca ctggtttaat tgaaccattt
      901 cacgaattca tgaggcggaa tcgcgtaaaa tgacccctcc cctcattacc ctctccatcg
      961 tcagtcatgg cgatgcacag aagatttctc atttactagc cagtctgcaa aaacatgagc
     1021 agaataccgc atcacggttt caattgatcc ttacagacaa tctaaaagac aatttaccag
     1081 atgtagatcc taatcct
//
LOCUS       DAOREF010000968         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_149922_length_1096_cov_4.719500, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000968 DAOREF010000000
VERSION     DAOREF010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149922_length_1096_cov_4.719500"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKI33_16095"
     CDS             complement(<1..429)
                     /locus_tag="PKI33_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38594.1"
                     /translation="MLTKTSRVLTYLLAALYAVIGGVLFFRPEQQAPIFAWNVTGFMT
                     MTIGAWCLGNAWLAFFTARRWNWQLVYPALSYLWSFGLLEGMIVVSFREKLNLDYPVA
                     WFYVSVISLNAVAAVFGIIDWLRVRPAGNQSEPMTGLMRGV"
     gene            complement(506..>1096)
                     /locus_tag="PKI33_16100"
     CDS             complement(506..>1096)
                     /locus_tag="PKI33_16100"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNM38595.1"
                     /translation="YEILKLDAHVTGVDIKADLIERHQEKARKLGWSQLEFKVGTIGD
                     FVPASTPDIVVALHACDTATDDALANGIRWQSKVIVCAPCCQHELQVQMAQVPAPTAL
                     ASVLSHGILFERMGDILTDTFRATLLRIMGYRTDVTQFVPIEHTAKNLMIRSVKVSPA
                     GKNPRWLEEYKNLKSFWQVTPYLEKLLGENYAQYVE"
BASE COUNT          284 a          285 c          292 g          235 t
ORIGIN      
        1 aaccccgcgc atgagaccag tcatcggctc agattgattc cccgccgggc ggacacgcaa
       61 ccagtcgatg atgccaaaca cggcggcaac ggcgttgagg ctgatcacag acacatagaa
      121 ccaagccacg gggtaatcta aattcagctt ctcacggaaa gacacaacga tcatgccttc
      181 caacaagcca aaggaccaga gataagacaa ggctgggtag accagttgcc agttccagcg
      241 gcgagccgtg aagaacgcca gccaggcatt gcccaagcac caggcgccga tggtcatggt
      301 catgaagccg gtcacattcc aggcaaagat cggcgcttgt tgttcggggc gaaaaaacaa
      361 gactccgcca atgacggcgt agagcgcggc gagcagatag gttaacacac gagatgtttt
      421 ggtaagcata attctctcct ctattaaagt ttggaacaaa agatataaac actacgttgg
      481 tgcttgagtt ttcaccccaa aaggatcatt ctacatactg agcataattt tcgcccaata
      541 acttctccaa atacggtgtg acttgccaga agcttttgag gtttttgtat tcttccagcc
      601 agcgcggatt cttgcccgcg ggcgaaactt tcaccgagcg gatcattaga ttcttggcgg
      661 tgtgttcaat gggcacaaat tgagtgacat cggtgcgata gcccatgatg cgcaatagcg
      721 tagcacggaa ggtatcggtg aggatatcac ccatacgctc gaacaagatg ccgtgactga
      781 gcaccgaagc aagcgccgtc ggtgcaggga cttgtgccat ttgcacctgt aactcatgct
      841 gacaacatgg cgcacagaca atgaccttgc tctgccagcg gatgccattg gcaagcgcgt
      901 cgtcggtggc ggtatcacaa gcatgcaggg caaccacgat atcgggtgtg gaggcaggca
      961 caaagtcgcc gatggtgccg accttgaatt ctagttgact ccagccgagc tttctggctt
     1021 tttcttgatg gcgttcgatc aggtccgctt tgatatccac gccggtgacg tgcgcatcga
     1081 gcttcagaat ttcata
//
LOCUS       DAOREF010000969         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_152467_length_1087_cov_2.972868, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000969 DAOREF010000000
VERSION     DAOREF010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152467_length_1087_cov_2.972868"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..881)
                     /locus_tag="PKI33_16105"
     CDS             complement(150..881)
                     /locus_tag="PKI33_16105"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNM38596.1"
                     /translation="MRTKYFAFTILAVLALVLGACGPTTINQAAPTTDRIMNVNGLGV
                     VYLTPDIAYINIGVGTQKPDAAEAVEANKAQTTKVIQAIKDSGVDDKDIRTTNFSIWS
                     NPQYDPAGTGQILSTSYSVDNTVNVTIRDLDKLGDLLDAAVQAGANNIYSIQFDVDNK
                     NDANKEARTLAVENARMQAQELAAATGVELGEIQSISYYESSPTQYFEGKGGGGGGDV
                     ASAAVPIQPGQLAISVNVNITYFIK"
BASE COUNT          277 a          250 c          275 g          285 t
ORIGIN      
        1 taaccaactc ttaacatgaa gagactcctt gagtgagaat ggtgagatta taacggcact
       61 gccagatttg ccgcgatcta cgaaaaggta gaggatgtaa aaagcctgcc tctttgcaga
      121 gacaggcttc aggtccgaac gtgaggcgtt tacttaataa agtaggtgat gttcacattg
      181 acactgatgg caagctgacc cggttggatc ggcaccgcag cgctggcaac atccccgcca
      241 ccaccgccac ccttgccttc aaagtattgg gtggggctgc tttcgtagta gctaatggac
      301 tggatttcgc ccaattccac accggtcgca gcggcaagtt cttgtgcttg cattctcgcg
      361 ttttccacag ccaaggttcg ggcttctttg ttggcatcgt tcttgttatc cacatcgaac
      421 tgaatgctgt agatgttgtt tgcaccggct tgcacggcgg catcgagcag gtcgccgagt
      481 ttatccaggt cacgaatggt cacattgacg gtgttatcca cagagtagct ggtgctaaga
      541 atctgaccag ttcctgccgg atcgtactgc gggttcgacc aaatgctgaa gttggtggtg
      601 cgaatatctt tgtcatccac acccgagtct ttgatcgcct gaatgacttt ggtggtttgt
      661 gctttgttag cttccacagc ttcagccgca tctggctttt gagtgccaac gccaatattg
      721 atataagcaa tatcgggggt aaggtataca acgcccagcc cgttgacgtt cataatgcgg
      781 tccgttgtag gggcggcttg gttaatggtg gtcggtccac aggcgcccaa gacgagtgcc
      841 agaactgcca aaatggtaaa agcaaaatat ttcgtacgca tggtaatctc cttattattg
      901 attatgcact attatgacga cactttcata taaaagttcc cgtacaatcg cttgaatttt
      961 gactcgatac cgactagaat tgcacaaatg ttctagggat tacaatgccg attaactatc
     1021 aggaaatcta cacacagatc aaacaagtgg gtttgggcgc aaaagaaaga aggaagaaga
     1081 aagagga
//
LOCUS       DAOREF010000970         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_154360_length_1081_cov_2.197856, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000970 DAOREF010000000
VERSION     DAOREF010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154360_length_1081_cov_2.197856"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKI33_16110"
     CDS             complement(<1..391)
                     /locus_tag="PKI33_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38597.1"
                     /translation="MPTRPQLSISKTDYKVARSCAAKLYYRMNKYPSSKDGDEYIELL
                     AEGGYMIGAIASLLFEDAVLVDEPDHDQAVALTQEYFKHENIILLEAAFESQRKFARP
                     DILIKQGDTLTLIEVKAKSFDSSVENPI"
     gene            complement(400..621)
                     /locus_tag="PKI33_16115"
     CDS             complement(400..621)
                     /locus_tag="PKI33_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38598.1"
                     /translation="MSETIIINLTKTVVAEMTKSYLERQYPEYSFKVVTYYSELKHVV
                     THQPIEGEQLQQMQAYAQGVSDVLSKLLA"
     gene            complement(699..>1081)
                     /locus_tag="PKI33_16120"
     CDS             complement(699..>1081)
                     /locus_tag="PKI33_16120"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNM38599.1"
                     /translation="ILVNEEREFLINVSERLGVSLDIEQVEERTKEYQVIVEKNIIER
                     TAGAMGSAAVSVIGTAGQAATKIKETLGKAVKRDGSDKASSSPAQKHNDPVDKLKKLK
                     QMLEEGLITQDEFDAKKAEILSGI"
BASE COUNT          233 a          234 c          254 g          360 t
ORIGIN      
        1 gaatgggatt ctctacgctg gagtcgaacg atttggcttt gacctcgatc agggttaggg
       61 tatcgccttg cttgatgagg atatctgggc gtgcgaattt tctctggctc tcgaaagcgg
      121 cttcgagcag gatgatgttc tcgtgcttga aatattcctg ggtgagcgcc acggcttggt
      181 cgtggtcggg ttcgtccacc aggactgcat cctcaaagag cagactggcg atcgcgccga
      241 tcatgtatcc gccttcggcg agcagttcga tgtattcatc cccgtctttg ctggacgggt
      301 atttgttcat gcggtagtac agcttggcgg cgcaggagcg cgcgactttg taatcggtct
      361 tagagatgga aagttgcgga cgggtgggca tgggtctatt tatgccaata gcttacttaa
      421 tacgtcagaa accccttgag catatgcctg catttgttgc agttgctctc cttctatagg
      481 ttgatgggta actacgtgtt tgagttcact gtagtaagtg actactttaa aagagtattc
      541 tgggtattga cgttccaaat aggattttgt catctcagct acaacagttt ttgtaaggtt
      601 aatgataatg gtttcgctca ttggaatatt ggaatgtccc taattactct gttctttcat
      661 tatacaaaaa aaagatattt atttaccaga agttcttctt atataccaga taagatttct
      721 gctttttttg cgtcaaactc atcttgagta atcagaccct cttcaagcat ctgttttagt
      781 tttttgagtt tgtccacagg gtcattatgt ttttgtgcgg gggagctaga agccttatcg
      841 ctaccatctc gttttactgc tttcccgaga gtttccttga tcttggtggc ggcttggcct
      901 gctgtaccta taacacttac ggcggcactg cccatagccc ctgctgttct ttcaataatg
      961 tttttctcaa cgataacctg atattctttt gttcgttctt cgacttgctc gatatccagt
     1021 gagaccccaa gtctctcgga gacatttatc agaaactccc gttcttcatt aactaatatg
     1081 c
//
LOCUS       DAOREF010000971         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_156644_length_1073_cov_4.675835, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000971 DAOREF010000000
VERSION     DAOREF010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156644_length_1073_cov_4.675835"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKI33_16125"
     CDS             complement(<1..482)
                     /locus_tag="PKI33_16125"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNM38600.1"
                     /translation="MSDEYIPKVAMSIQAHPDDQEFGIGGTIAKWTKAGCEFITVVIT
                     SGDSGSNDPSKGADYKPVLAELREKEQLAANEVLGVKETVFMRYPDGELEPTIALRKE
                     LTRLIRKYKPDVVLAGNPEAWFYGNEYLNHADHRAAAGAGCEAVFPSAGSRLIFAELL
                     S"
     gene            808..>1073
                     /locus_tag="PKI33_16130"
     CDS             808..>1073
                     /locus_tag="PKI33_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNM38601.1"
                     /translation="MSSTLAIETKDLGRIYKLRGSKKETRKELVALQGVDLTVERGEL
                     FGLLGPNGAGKTTLIKILTTLLAPTSGWARVAGADVHAEPESVRP"
BASE COUNT          287 a          286 c          239 g          261 t
ORIGIN      
        1 gacaacagtt cggcaaaaat gagcctcgac cccgctgaag gaaagaccgc ttcacaaccg
       61 gcaccggctg cggcgcgatg atcggcatgg ttcaaatatt catttccata aaaccatgct
      121 tcaggattac ccgccaaaac cacatcgggt ttatatttgc ggatgagacg agtgagttcc
      181 ttgcgaagcg caatggtcgg ctccagttcg ccatctgggt aacgcataaa gacggtttct
      241 ttcaccccta atacttcatt ggcagcaagc tgctcttttt cgcgtaattc cgccagtaca
      301 ggcttatagt ctgcgccttt gctggggtcg ttcgagccag agtcaccact ggtgatgacg
      361 actgtgataa attcacagcc tgcttttgtc cattttgcga tagtaccgcc aatcccaaat
      421 tcctggtcat ccgggtgcgc ctgaatgctc atggcaactt tgggtatgta ttcgtcactc
      481 ataaagtttt ctcctaaact ataggtgcca gccgctaaaa tgtcttgcac ctgaataaaa
      541 acttcagaga ttttacatca caaatgggag tcgtagtaaa atagaactta tattctagtt
      601 ttcctcttcg caatttatat aagccatgct ttgccattaa attgcgagag ccagggtcgc
      661 cttaccctgg ctctcttaca aaggaggaga agagaatttt gttacgcatg taaatttatc
      721 acgcctgccc caagagcgct tgacgcaaac cctacgccca acccacgcaa acacaacgaa
      781 tcagattaca aaattttaag gagaatcatg tcctcaacac tcgcaatcga aacaaaagac
      841 cttggtcgca tttataaatt gcgtggcagc aaaaaagaaa cacgcaaaga attggtcgcc
      901 ttacaaggcg tggacttaac cgtggaacgc ggagagttat ttggactctt gggacccaac
      961 ggcgccggca agacgaccct catcaagatc ctcaccacgc ttctcgcccc gacctcgggc
     1021 tgggcacgtg ttgcaggagc cgacgtccac gctgaacccg agtctgtgcg ccc
//
LOCUS       DAOREF010000972         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_157250_length_1071_cov_3.821850, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000972 DAOREF010000000
VERSION     DAOREF010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157250_length_1071_cov_3.821850"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1071
                     /locus_tag="PKI33_16135"
     CDS             <1..>1071
                     /locus_tag="PKI33_16135"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNM38602.1"
                     /translation="LQAAGYKTGLYTSPHLEDFVERIRINGEPISHEQMVELVDEIKP
                     YVAKIEKLTTFEITTALAFMAFAKYGATAAVFEVGLGGRLDATNIVTPKVSVITSLSY
                     DHMAVLGNTLALIAGEKAGIIKPGVPVVSSPQKEEALEVLLRVAKDRDSEITLVGRDV
                     GFEMQGTSLEGQWLAIDDRRWTTDDGRSMVDGSSSILELHIPLLGKHQIENAATAYAA
                     LKTSGIPITDEQIKTGFSQVQWRARFEVARVEPPVIFDSAHNQDSFEKLRETLDEYFP
                     GKKVYLIFGASEDKNIPGMFAEMKPKIQKIMITRADHPRALSVEHVAGLADQAGAEWE
                     AVVPVKDALRRALELSAKDGSIV"
BASE COUNT          275 a          230 c          305 g          261 t
ORIGIN      
        1 ttgcaggcgg caggttacaa gactggttta tatacgtctc cacacctgga agatttcgtc
       61 gagcggattc gcatcaacgg cgagccaatc tctcatgagc aaatggttga attggtggat
      121 gagatcaagc cgtatgtggc aaagatcgaa aaactgacca cctttgaaat cacaacggca
      181 ttggcgttta tggcgttcgc aaaatatggt gccacagcgg cggtttttga agtcggcttg
      241 ggtgggcggc tggatgcgac caatatcgtc acgccaaagg tttcagtcat cacgtcactt
      301 tcgtatgacc acatggcggt attgggcaac accttggcac tcatagcagg ggagaaggct
      361 ggcattatca agccgggtgt gccggtggtt tcatctccgc agaaagaaga agctctcgaa
      421 gtcttactgc gcgtagccaa agatagagat tctgagatta ctcttgtagg gcgcgatgta
      481 gggtttgaaa tgcaaggaac gtctttggaa ggtcaatggt tggcgataga cgacaggcga
      541 tggacgacag atgatggaag gtccatggta gatggttcat cttccattct cgaactacac
      601 attccattac ttggcaaaca tcaaattgaa aatgccgcaa cagcctatgc agcgttgaaa
      661 accagtggaa ttccgatcac ggatgagcag ataaaaactg gcttttctca ggtacaatgg
      721 cgcgcccgct ttgaggtggc tcgtgttgaa ccgcccgtca tttttgactc ggctcataat
      781 caggattcgt tcgagaagtt gcgtgagacg ttggatgaat atttccccgg taaaaaagtc
      841 tatctcatct tcggcgcatc ggaagataag aatattcccg gcatgtttgc agagatgaaa
      901 ccgaagattc aaaaaattat gatcacacgt gccgatcatc cgcgggcttt gagcgtggag
      961 catgttgcgg gtcttgccga tcaggcaggg gcggagtggg aggcggtggt tcccgtcaaa
     1021 gatgctttgc ggcgcgcgtt ggaactatcc gcgaaagatg gtagcattgt a
//
LOCUS       DAOREF010000973         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_160380_length_1061_cov_3.849901, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000973 DAOREF010000000
VERSION     DAOREF010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160380_length_1061_cov_3.849901"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKI33_16140"
     CDS             <1..208
                     /locus_tag="PKI33_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38603.1"
                     /translation="QTELNFEARKQIVWDMQKIVHDDVVYIIPFYDANVQAYRTDRFT
                     GWITDQPKVELSDVTSLTVIEPVK"
     gene            277..>1061
                     /locus_tag="PKI33_16145"
     CDS             277..>1061
                     /locus_tag="PKI33_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNM38604.1"
                     /translation="MDRTRAILRKAAWSVFTIFFVIILNFFLFRILPGDPARAGIRDP
                     RLNKDAVEALRVRFGLDKPIINCFESLNPIKIGSCSVNPLDTQFFIYVKNLLQGEMGF
                     SYHTNRPVVEVLSERLWNTILLIGVGQILAILFGVAFGIFAAWRARTSIDYASVGASL
                     VAWSLPTFWLAIVLLFWGSMIGLPVAGKATPGMSSYPLLQQWGDIAKHMLLPSITYTI
                     VYMGEYTLIMRSSLLDVLSEDYILTAKAKGLSTVQILRDHALK"
BASE COUNT          263 a          234 c          243 g          321 t
ORIGIN      
        1 acagactgaa ttaaacttcg aggctcgcaa acaaattgtt tgggatatgc agaagatcgt
       61 tcacgatgat gttgtgtata tcattccgtt ttatgatgcc aatgttcaag cttatcgcac
      121 agatcgtttc actggttgga tcacagatca acctaaagtg gaactatctg atgtcacttc
      181 actgactgtt attgagcctg taaagtaaaa actttgaaat gaaagatgtt gcccaacatg
      241 aatattcatg ttgggcaacg ttcaagagag aaacccttgg atcgcactcg cgccattctc
      301 cgaaaagcag cttggtctgt ttttacaata ttctttgtca ttattctgaa tttttttcta
      361 tttcgcatcc tgcccggtga ccccgctcga gcgggaatcc gcgacccgcg tttgaacaaa
      421 gatgcggtgg aagcgttgcg cgttcgcttt gggttagata aaccaattat caattgcttt
      481 gagtcgctca atccgatcaa gatcgggagc tgctcggtta atccactcga tacacaattc
      541 ttcatttatg tgaaaaatct tctacaaggc gaaatgggat ttagctatca cacgaaccgt
      601 cccgttgtag aagtccttag cgaacgattg tggaacacga ttttgttaat tggagtagga
      661 caaatccttg ccatcctttt tggggtggca ttcggtattt ttgctgcttg gcgcgcgcgc
      721 acttccatag attacgcttc cgtcggcgca agtttggtcg cgtggagttt gccgaccttt
      781 tggcttgcta tcgttttgct cttctggggc agtatgatcg gcttacctgt tgcgggtaaa
      841 gccacacccg gtatgagttc gtatccgcta ttgcaacaat ggggcgatat tgcaaagcat
      901 atgcttctgc cttctattac atacacgatc gtatatatgg gcgaatacac cctgattatg
      961 cggtctagct tgttagatgt gctttctgaa gattacattc tgaccgccaa agccaaagga
     1021 ttgagcacgg ttcaaatttt gcgagaccat gccctcaaga a
//
LOCUS       DAOREF010000974         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_160399_length_1061_cov_2.849901, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000974 DAOREF010000000
VERSION     DAOREF010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160399_length_1061_cov_2.849901"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1061)
                     /locus_tag="PKI33_16150"
     CDS             complement(<1..>1061)
                     /locus_tag="PKI33_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNM38605.1"
                     /translation="AIIMVRDISQRKWVETEREKLINELEEKNSESEALREGMTILVE
                     TLDETKAVSLILEQLEKVLPFDSASVQLLKGEVLEIVSTRHLEPREMHLGTRYVVSEK
                     EPSYPLLSGKVPYVLVKDVRATFTDLFAVEPYTTIRTWLAVPLRIKRQIIGIITLDGY
                     QPNKFTQRHAELALTYASQVAVALDNARLFSELQVELAARKNLIEELEVKNAELERFT
                     YTVSHDLKSPVITIRGFLGFLEQDALSGNVTRLRGDIQRIADATDKMQTLLNELLELS
                     RIGRLVNPPNLVPFSEIVYEALDLVLGRIQKNHVQVQIQDEMEAVYVDRQRMVEAVQN
                     LIDNAAKFVGESPKIEIGQ"
BASE COUNT          285 a          249 c          226 g          301 t
ORIGIN      
        1 tttgaccaat ttcgatcttg ggactttctc ccacaaattt ggcggcatta tcgattaggt
       61 tttgaacggc ttccaccatc cgttgacggt ctacatagac cgcttccatc tcatcctgaa
      121 tttggacttg cacatgattc ttttgaatac gccctaaaac cagatcaagc gcttcataga
      181 caatctcact aaacggaacc aaatttggcg ggttcaccaa acgtccaatc cgtgaaagct
      241 caagcaattc attcaagaga gtttgcattt tgtcagtggc gtcagcgatg cgttggatat
      301 caccccgtaa gcgggttaca tttccgctaa gtgcatcctg ttccagaaaa ccgagaaaac
      361 ctcgaattgt aattacagga gatttaaggt catgagagac tgtgtaggta aaccgttcca
      421 gttcggcgtt tttcacttca agttcttcga tcaagttctt gcgggcagcc aactccactt
      481 gcaattcact aaacagacgg gcattgtcca aagccactgc tacttgactg gcatacgtta
      541 gagcaagttc ggcatggcgc tgagtaaatt tattaggctg atatccatcc aaagtaatga
      601 tgccgatgat ctggcgttta atacgcaagg gaacagcaag ccaggtacga attgtggtgt
      661 agggttctac tgcaaataga tctgtgaacg ttgctcgtac atcctttaca aggacataag
      721 ggactttgcc cgataaaagc ggataagacg gttctttttc actgaccaca taccgcgtgc
      781 ccaaatgcat ctctctgggc tcaaggtgtc tggtgctaac gatctctaaa acttcccctt
      841 ttaagagttg tacagaggca gaatcgaagg gaagcacctt ttcaagttgt tcaagaatta
      901 agctgacagc tttagtttca tccaaggtct caacaagaat ggtcatacct tcgcgcaaag
      961 cttcagattc agagttcttt tcttccagct cgttgattaa tttttcacgt tcggtttcca
     1021 cccatttgcg ttgactaata tcacgcacca tgatgatggc a
//
LOCUS       DAOREF010000975         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_162793_length_1053_cov_3.431864, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000975 DAOREF010000000
VERSION     DAOREF010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162793_length_1053_cov_3.431864"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKI33_16155"
     CDS             complement(<1..612)
                     /locus_tag="PKI33_16155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNM38606.1"
                     /translation="MNIYISLLQLRPKYRLLWLAQVISLAGDWFNTIASVIIVNRYTT
                     SGLALGGLFIARALPPFLLGPVAGVVADRFDRRKVLIASDILRAVIVLCFLLVDRPER
                     LWLMYVLTVLQFSVSTFFEPARAALTPALVETDELLNANTLASITWSAMLTLGGAIGG
                     VTASYFGVPVSLVVDAASFIVSAMLVASITGEFRSQMAHALESG"
     gene            complement(727..>1053)
                     /locus_tag="PKI33_16160"
     CDS             complement(727..>1053)
                     /locus_tag="PKI33_16160"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HNM38607.1"
                     /translation="IFFVIHDPTTLNRQGADVGTQYRSGIYYHTPEQKEVAEQTIRDL
                     EAQKVWGSRIVTEVEPIKDFYIAEDYHQEYFVRNQSQPYCQAVVAPKVGKFRKHFLEL
                     WKKQAA"
BASE COUNT          298 a          266 c          255 g          234 t
ORIGIN      
        1 gccgctttcc aatgcatgtg ccatctgcga acggaattcg ccggtgatgc tggcaaccaa
       61 catggcggaa acaataaaac tcgccgcatc cacaacgagt gaaacgggca cgccaaaata
      121 ggaggctgtc actccgccaa tggcaccgcc caacgtaagc atcgcagacc aagtgatact
      181 cgccaaggta ttggcattga gaagttcatc ggtttcaacc aaggctgggg tgagcgctgc
      241 tcgtgccggt tcaaaaaaag tagaaacact aaattgcaag acggtgagca catacataag
      301 ccatagccgc tcaggtcggt ccaccagcag aaagcaaagc acgatcacgg ctcgcagaat
      361 atcacttgca atcagcactt tgcgtcggtc aaagcggtca gctacaactc cggcaacggg
      421 tcccagcaaa aatggcggaa gcgcacgggc aatgaataac cctccgagcg caagcccgga
      481 tgttgtatag cgattaacaa tgatgaccga agcgatggtg ttaaaccaat cgcctgcaag
      541 gctgatgacc tgtgccagcc aaagcaatct atattttgga cgaagttgga gaagggagat
      601 gtagatattc actcaggcaa ttatagccgt gagaacaatc attccacttc gatttgaaat
      661 tcgaataaat aaaaagggac gcagctcagt attcaactgc gtcccttttt atatgatgct
      721 tacgaactac gctgcttgct tcttccacaa ttccaagaag tgtttgcgga acttaccaac
      781 cttcggtgca accactgcct gacaatatgg ctgagattga ttccgcacaa aatattcctg
      841 atggtaatct tcagcgatgt agaaatcctt gatcggctcc acttcagtga cgatgcggct
      901 accccaaacc ttctgcgctt ccaaatcgcg gatggtctgt tcggcgactt ccttttgctc
      961 gggtgtgtga tagtaaatgc ctgagcggta ttgtgtgccc acatccgcac cttggcgatt
     1021 caatgtagtc gggtcgtgaa tgacaaaaaa gat
//
LOCUS       DAOREF010000976         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_163492_length_1051_cov_2.645582, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000976 DAOREF010000000
VERSION     DAOREF010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163492_length_1051_cov_2.645582"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKI33_16165"
     CDS             complement(<1..488)
                     /locus_tag="PKI33_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38608.1"
                     /translation="MQTDTGIDTQLKEAVQLTGADWAVLAERVGGVWLVRASYHLTKA
                     AQNELTGIMAAASTDAWLCGALSGGHSRSAEIPENRSIGEGRFLAFPILSTSKLLLVG
                     AESEQSNAAQRIWRLTASLLSGQFTPKTQTLLPDLQSGLAFDLPLALEKVLTNFTQTV
                     SC"
     gene            547..>1051
                     /locus_tag="PKI33_16170"
     CDS             547..>1051
                     /locus_tag="PKI33_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNM38609.1"
                     /translation="MNEVTVIGGGLAGSEAAWQLAQQNIKVKLYEMRPVNPTGAHVTS
                     DLAELVCSNSLGSNQADRASGVLKNELRRLNSLLVECAEDTALPAGAALAVDREAFAK
                     RVTERVEAHPNIELVREEVKEIPSSPVIVASGPLTSDSLSKSIAALSGDEHLFFFDAI
                     SPIVRAES"
BASE COUNT          259 a          281 c          281 g          230 t
ORIGIN      
        1 tggcaagaga cggtttgcgt aaaattggtg agaacctttt cgagcgcaag tggaagatcg
       61 aaagccagac ccgattgaag gtcaggaaga agcgtttgcg tcttcggagt gaattgccca
      121 gaaagcaaag aggcggtcaa tcgccagatc cgctgggcgg cgttggattg ttccgactct
      181 gcgccgacga gaagcaactt ggatgtgcta agaatgggaa aagcaagaaa gcgtccctca
      241 ccgattgacc gattttctgg gatttccgcc gaacgagaat gccccccgct gagcgcgcca
      301 cacaaccaag catcagtaga ggctgccgcc ataataccgg tcagttcgtt ttgagcagct
      361 ttggtgagat gatacgaagc ccgcaccagc catacaccgc caacacgttc tgccaacacc
      421 gcccagtctg caccggtgag ttgaaccgct tctttgagtt gagtgtcgat gcctgtgtct
      481 gtttgcatgg taaatgctat tcaaattata cattatgccc atcgtccact ggtaaaatag
      541 cgccctatga atgaagtgac tgttatcggt ggcgggcttg ccggaagtga agcggcatgg
      601 cagcttgccc agcaaaatat caaagtaaaa ctctatgaga tgcgcccggt caacccgacc
      661 ggtgcccatg tgaccagtga cctagcggaa ctggtctgtt cgaattcact cggctccaac
      721 caagccgacc gcgcctcggg cgttctgaaa aatgagctac gccgactcaa ttccctgctg
      781 gtggagtgtg ctgaagatac agccttgccc gctggcgctg ctttagcggt agaccgtgaa
      841 gcctttgcaa aacgcgtcac agagcgagtg gaagcgcatc ccaatatcga attggtccgt
      901 gaagaagtga aagagattcc atcttcgcct gtcatcgtcg ccagcggacc attaacctca
      961 gatagtctct ccaagtcgat tgcggcattg agcggtgatg aacacctctt cttcttcgat
     1021 gccatttcac ccatcgtccg cgccgaaagc a
//
LOCUS       DAOREF010000977         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_167122_length_1040_cov_2.269036, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000977 DAOREF010000000
VERSION     DAOREF010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167122_length_1040_cov_2.269036"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..774)
                     /locus_tag="PKI33_16175"
     CDS             complement(76..774)
                     /locus_tag="PKI33_16175"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNM38610.1"
                     /translation="MKISPQDLKQVVVFQHATDEDLELILKNSITRSIEEGSFFFMQG
                     DEAHYLYILTSGQVKLMQSNPNGQQVNLRTIYPWQMFGALGAVRTDGAAYPASAQTLE
                     DSTALATPSLFLRSMLETRPYLSFDLMNLMTSYIQEMQARYRELATERVEQRVANALV
                     RLAGQSGIRSDKEAGIELSFSRQDVAEMTGTTLYTVSRLLSDWERKGIIKTGREKILV
                     LNPHELVRIAYGQV"
     gene            915..>1040
                     /locus_tag="PKI33_16180"
     CDS             915..>1040
                     /locus_tag="PKI33_16180"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HNM38611.1"
                     /translation="MENEATLRRLKTVEGHLRGVIRMVEEDAYCIDVIRQIQAIEA"
BASE COUNT          242 a          262 c          281 g          255 t
ORIGIN      
        1 gctacagaat atcgcattcc cccaaaccct tctttgcgct ggcgcaaata aaaagagcgc
       61 aaccttgctg cgcccttaca cttgtccgta tgctattctt accaactcat gcgggttgag
      121 aaccagtatc ttttcccgcc cggtcttgat gatgcctttc cgctcccaat cgctgagcag
      181 acgactgact gtgtacagcg tggtgccggt catctctgcc acatcttgcc gggagaagga
      241 tagttcgatc cccgcttctt tatcggaccg tatgcctgat tgcccagcca agcggaccag
      301 cgcgttagcc actctctgct cgacccgctc ggtagcaagt tcgcggtaac gtgcctgcat
      361 ctcttggatg tagctggtca ttaggttcat taagtcaaag gaaaggtagg ggcgagtttc
      421 gagcatggag cgcagaaaca ggctcggggt cgcaagagcc gtgctgtcct ccaaggtttg
      481 agcggaagca gggtaggcgg ctccatccgt gcggacggct cccaatgcgc cgaacatctg
      541 ccacgggtag atcgtccgca ggttcacttg ttgcccgttg ggatttgact gcatgagctt
      601 gacctgtccg ctggtgagga tgtacagata atgggcttca tcaccctgca taaagaagaa
      661 actgccttct tctatggagc gggtaatgct attttttaga ataagttcaa gatcttcatc
      721 ggttgcgtgt tgaaaaacaa cgacctgttt gagatcttgg ggggaaattt tcataaagtc
      781 attttacacc ccaatccgat gacatttatc gacttgacag aggtgtgggg gatggcgtat
      841 attttgtcta cccctacccc cctggggggg gtaagagaaa ctcaacgacc tgtatctgaa
      901 aactagaggt aactatggaa aacgaagcga cacttagacg attaaagacc gtggaaggac
      961 acctgcgtgg cgtgatccgc atggtggaag aggatgccta ttgcatcgac gtcatccgtc
     1021 agatacaggc gattgaggcg
//
LOCUS       DAOREF010000978         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_167349_length_1039_cov_3.734756, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000978 DAOREF010000000
VERSION     DAOREF010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167349_length_1039_cov_3.734756"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1004)
                     /locus_tag="PKI33_16185"
     CDS             complement(210..1004)
                     /locus_tag="PKI33_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38612.1"
                     /translation="MELNFATITLPTIVVWMLGLFIFLLGSLLGYFNMSIDARKKQEL
                     AEQKVEHARIEADRRIAEMQKRLDEADIQPAKVQQTATTPSLLTLKSVNNLQTQIEMD
                     GQLLIAPLSPERKKRLVELINHFRPWLEGAATSQPISKPLTTPLSGPPTDLKKPEAVP
                     YVPPTVKPVPVILGVNNQKTKTDPESEFKLLSMVKQIDVVLQKRLAGTSLESLGIHLN
                     DSLQGGLEVQIGSQKFETIDDVPDANIKAAIRAAIAEWEQKYIPGQ"
BASE COUNT          272 a          203 c          246 g          318 t
ORIGIN      
        1 gcctgaaagt gtgatggtct atgccgaatg ggcaagacat agtctgtttt cgtacactct
       61 ggcggctttg gtcgctttgc atgtgggcgc ggcgttatat catcaattta ttcgcaagga
      121 taatttgttg tctcgtatgt ggtacgggaa gtaaaaaaag aatataaaag caaaaagccc
      181 tgctcacaag agcagggctt ttttcgaaat tattgtccag ggatatactt ctgttcccat
      241 tctgcaattg ctgcacggat ggcagctttg atatttgcat cgggtacatc atcaattgtc
      301 tcaaattttt gtgaaccaat ctgcacttcc aagccacctt gtaatgagtc gttgagatga
      361 atgcccagag actctagaga cgtccctgcc aacctttttt gtaatacaac gtcgatctgc
      421 ttaaccatgc ttagtaattt gaactcagac tcagggtctg ttttggtttt ttgattattc
      481 actccaagaa tgacaggaac tggctttaca gtaggtggca cataaggaac agcctcaggc
      541 ttcttaagat cagttggagg tccagaaagg ggcgtagtga gaggtttgga aataggctgg
      601 gatgttgcag ccccttcaag ccacggacga aaatgattga taagttccac taaacgtttt
      661 tttcgttcag gagaaagagg ggcgattaat agttgcccat ccatttcgat ttgagtttgt
      721 agattattta cgctttttaa tgttagcaaa gaaggtgtag tggcagtttg ctgaaccttg
      781 gcaggctgta tatcagcttc atctaaccgt ttttgcattt cagcaattct acgatctgct
      841 tcaatacgcg catgttctac cttttgttct gccagttctt gtttcttgcg cgcgtcaatg
      901 ctcatattga aatagccaag taagctgcca agtaaaaaga taaaaagtcc tagcatccat
      961 acaacgatgg tgggaagagt gatagtggcg aaattaagct ccatggttat ttcctttcaa
     1021 gcggctggca ggttgggca
//
LOCUS       DAOREF010000979         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_167670_length_1038_cov_3.805697, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000979 DAOREF010000000
VERSION     DAOREF010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167670_length_1038_cov_3.805697"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /gene="hppD"
                     /locus_tag="PKI33_16190"
     CDS             <1..974
                     /gene="hppD"
                     /locus_tag="PKI33_16190"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNM38613.1"
                     /translation="SGLETGNRDFASYVLESGQARFVVSAPYSPSSPLASHHMVHGDG
                     VKVIALEVDDVEKAWRETTSRGGKSAWAPREEKDDHGIYRSSAIYTYGETIHAFIDRD
                     DYKGTFAPTYRPLKHEAESTGLAAIDHIVGNVQLGKMNHWVNFYHQVMGFRQLMHFDD
                     KDISTEYSALMSKVMQNGNGRVKFPINEPADGKRKSQIEEYLDYYMSPGAQHIAIITG
                     DILSTVESLRKNGVEFLRVPDTYYEMLPDRVGKIKEDFKTIHELGILVDKDDEGYLLQ
                     IFTRPIQDRPTMFIEIIQRHGAQGFGKGNFKALFESLELEQERRGNL"
BASE COUNT          281 a          274 c          232 g          251 t
ORIGIN      
        1 attcagggtt agaaactggc aaccgcgact tcgcctcgta tgtacttgaa tcggggcaag
       61 cgcggtttgt tgtttctgca ccttattccc catccagccc gctcgcatca caccacatgg
      121 ttcatggcga tggcgttaaa gtcattgcgc ttgaagtgga tgatgttgag aaagcctggc
      181 gtgaaaccac cagccgcgga ggaaaatctg cttgggcacc gcgtgaagaa aaggatgatc
      241 atggcattta ccgcagttct gccatttaca cctacggcga aactattcac gcctttattg
      301 accgtgatga ctacaaaggc accttcgccc caacctatcg tccacttaaa catgaagcgg
      361 agtctaccgg gcttgctgcc attgaccaca ttgttggcaa cgtgcaactc ggcaaaatga
      421 atcactgggt aaatttttat catcaggtaa tgggcttccg tcaactcatg cacttcgacg
      481 ataaagacat ctccaccgaa tactccgccc tcatgtccaa ggtcatgcaa aacggaaatg
      541 gacgcgtcaa gttccccatc aatgaacctg cagatggaaa acgaaagagt cagatcgaag
      601 aatatctcga ctattacatg tctcccggtg cgcaacatat tgccataatc acaggcgata
      661 ttctcagcac tgttgaaagt ctccgtaaga atggtgtcga atttttacgc gtgccagata
      721 cttactatga aatgctgcca gaccgtgttg gcaagatcaa agaagatttc aaaaccatcc
      781 acgaactcgg cattcttgtg gataaagatg atgaaggcta tctgctgcaa atcttcacac
      841 gtcccattca agaccgccct accatgttca ttgagatcat tcaacgccat ggcgcgcagg
      901 gtttcggcaa aggcaacttc aaagccttgt tcgagtctct tgagttggag caagaacgcc
      961 gcggcaatct ttagcgcatg agcttctgcg gataaaaatc atcgcctcac accagacccg
     1021 ctgcctgtat cccatttc
//
LOCUS       DAOREF010000980         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_168095_length_1037_cov_2.272912, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000980 DAOREF010000000
VERSION     DAOREF010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168095_length_1037_cov_2.272912"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>1037
                     /locus_tag="PKI33_16195"
     CDS             51..>1037
                     /locus_tag="PKI33_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNM38614.1"
                     /translation="MFDYVPNELDPISFWVLLLLSGGQHFTFHDLVKTMDRSKESVWD
                     AIQNLQSKKYAKNANSKFMIDFAGVEYLTKRGILANEAEETPKPKDFKKKLKPPFFSN
                     WTEMLIWLALPFSLSLIATILTIYFTYGYNYVSPERRMYQIATWIVVAVLLFFYVKLS
                     YHHPMGRERIVVFQSGEAIGKKGPGRILLLPLIQHPKKVDLSERPLEIKKEPCLTRDK
                     MLVTAGFYMTWEIDDPILSLTKVSNVEDSISSLTAAVLRSTIAEYEMNDAIEKQRSLN
                     ALIRTRIEHKATEWGVQVNNTELRELQPPDGVIKSIENRRKSENNIESLRQLM"
BASE COUNT          304 a          243 c          214 g          276 t
ORIGIN      
        1 taatgcccgt attatcaacg gttcactcaa ctgcattcaa cgaggacatc atgtttgact
       61 acgttcccaa cgaacttgac cccatttctt tctgggtctt attactgctt tctggtggtc
      121 aacactttac ttttcacgat ctggtcaaaa ccatggatcg ctccaaagaa agtgtatggg
      181 atgcgataca gaatttacaa tccaagaagt acgccaaaaa cgcaaactca aaatttatga
      241 ttgactttgc cggggtcgaa tatctcacaa agcgcggtat ccttgccaat gaagcagaag
      301 agacgcccaa acccaaagac tttaaaaaga agttgaagcc gcctttcttc tccaactgga
      361 cagaaatgct catttggctg gctctgccgt tcagcttatc gctaatcgca accattctaa
      421 cgatttattt cacatatggg tacaactatg tctcgcccga aaggcgcatg tatcaaatcg
      481 ctacatggat cgtcgtagcc gttctgcttt tcttctatgt gaaattgtca taccatcacc
      541 ccatgggcag agaacggatt gtggtattcc agtctggtga agccatcggc aaaaagggac
      601 cagggcgcat tttattactc ccgctcattc aacaccccaa aaaggtagat ctcagtgagc
      661 gcccgcttga aattaaaaaa gaaccctgcc tgactcgaga caagatgttg gtcacagccg
      721 ggttttacat gacatgggaa attgacgatc cgatccttag cttaaccaag gtatccaacg
      781 ttgaagattc aatttcatct ttgactgctg cggttttaag atctaccatt gcagaatatg
      841 aaatgaatga tgccatcgag aaacaacggt ctttgaatgc gctcattcgt acacgcatcg
      901 aacataaagc aacggaatgg ggtgttcagg ttaacaatac tgaacttcgt gagctccagc
      961 cgccagatgg cgttattaag tctattgaga accgccgtaa atctgagaat aatattgaat
     1021 ccttaagaca gttgatg
//
LOCUS       DAOREF010000981         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_168331_length_1036_cov_4.020387, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000981 DAOREF010000000
VERSION     DAOREF010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168331_length_1036_cov_4.020387"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..>1036)
                     /locus_tag="PKI33_16200"
     CDS             complement(258..>1036)
                     /locus_tag="PKI33_16200"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNM38615.1"
                     /translation="NTFRISAKDGMTQIEIPAGTFTMGAMDVYRENDEQPPHDVTLDT
                     YWIDQVEVTNGMYALCVTAGDCRPPSELRSDNRQEYFNNPDFRDYPVVYVTWFDADAY
                     CQWAGRRLPTEAEWERAARGDDKRNYPWGDELPNEYNSNSENTVGDTSRVGSYAEGAS
                     PFEVLDMAGNVWEWVADRYDPNYYAKSPAENPQGPNADEVQNIYRVIRGGSFQEDGLF
                     LRLANRSFLEGPNPKADPKTEENYGKSTNRIGFRCAQSGE"
BASE COUNT          248 a          244 c          251 g          293 t
ORIGIN      
        1 tgcaataatg gtaaggatag ggatgatttt tttcatggag actcctttgt ttgatgtctc
       61 cagtatatgg ataaagaaaa atagcctaat cccccaaagg ttgttaaatc agatgccagt
      121 gtcttacata tttgcaaact aggtggataa gtccccgctt ctctcttaac tggaggctga
      181 ttcagcgaca ttaaataaat caccccgtaa tcggatactt atctatccaa attacggggt
      241 gatttttggt ttcaaactta ttcgccactt tgagcacaac ggaagccaat gcggtttgtg
      301 ctcttgccat aattttcttc tgtttttggg tctgcctttg ggttgggacc ttccaagaag
      361 cttcggtttg caagccgtag gaagagacca tcttcctgaa atgaaccgcc acggatgaca
      421 cgataaatat tttgaacttc atccgcgttg ggaccttgtg ggttttcagc cggagactta
      481 gcgtagtaat tagggtcata ccgatctgca acccattccc atacattgcc tgccatatct
      541 aacacctcga acggacttgc cccttctgca tatgagccta cacgcgacgt atcaccaact
      601 gtgttctcgg aattggagtt gtactcattg ggcaactcat caccccatgg gtagttgcgt
      661 ttgtcatcgc cgcgtgcagc acgctcccat tcagcttcgg tagggaggcg gcgacctgcc
      721 cattggcagt aggcatcggc atcaaaccaa gtgacataca cgacgggata atcacgaaaa
      781 tcagggttgt tgaaatattc ctggcggtta tccgagcgga gttcactggg cgggcggcaa
      841 tctcctgctg taacgcagag tgcatacatg ccgtttgtga cttccacttg gtcgatccaa
      901 tacgtatcga gggtaacatc gtgcggtggt tgttcatcgt tttcgcggta cacatccatg
      961 gcgcccatgg tgaaggtgcc agcggggatt tcaatttgag tcatgccatc tttggcagat
     1021 atccgaaatg tattgg
//
LOCUS       DAOREF010000982         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_168776_length_1035_cov_2.614286, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000982 DAOREF010000000
VERSION     DAOREF010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168776_length_1035_cov_2.614286"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKI33_16205"
     CDS             complement(<1..295)
                     /locus_tag="PKI33_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38616.1"
                     /translation="MKTILRVRYWRIVLFFARVTASIIFWDIFLRRIGLGVWAKNTRP
                     RRLQNIAVRFRALAIRLGGVMIKVGQFLSARLDVLPPEITDELSGLQDEVPPVD"
BASE COUNT          310 a          263 c          187 g          275 t
ORIGIN      
        1 agtccacagg cggaacttca tcctgcaatc cagaaagttc atccgtgatc tcgggtggca
       61 gcacatccaa acgtgccgaa aggaactgcc ccactttgat catcacaccg ccaagacgaa
      121 tcgccagcgc gcggaaacga accgcaatat tttgcagcct tcgcggacga gtgttttttg
      181 cccaaacgcc caacccaatg cgtcgtaaaa aaatatccca aaaaataatg ctcgccgtaa
      241 cgcgggcaaa gaacagaacg atgcgccaat agcgcactcg caaaatggtt ttcatgaagc
      301 ctccatgcac gacaattgta gtcctttctt tcttaaaaac acaatgagca tgcaatacta
      361 tttcaagtgt aaaatccact ctaaagttta ttcaaatata aagaaaggcg agcacaatgt
      421 atctagattc atttttgaaa atactattag gcttcgcaat tttattgcca tgctattcct
      481 tcattcgaag agagataaat ggggaattaa gaataaaaaa tagtcgtgtc ttttctccat
      541 taagtaaaag tccaatggac atattctatt tagggtttat tcttggagtt atagtgattt
      601 ttattggcat cggcgagtta ttacaataat ttagaaattc gcacttgaca actcatccct
      661 ctctcatgta aactgacaag gtacttaaat ctcaaagaaa ggatcgcact tcaaatgtcg
      721 ttgcaattac taaacctgcg taagaaatcc aaccgaataa caacccaccc cgaagtgcgc
      781 ctattgaccg tttaattacg gctcaatttc ctgtgtcttt cccaaacacg gcgcacccgc
      841 gccgtgtttt ttattgtctt ttatcgtcat tgcgagccgc gctcttgctt tttgcggcga
      901 agcaatctca gacttaatca taagattgct tcgtcgggct atcgccctcc tcgcaatgac
      961 gaaataaaac ggaaccaacc atgaacctct ccttcaaatc ctactggcac ctgctcgccg
     1021 accacatccg ccccc
//
LOCUS       DAOREF010000983         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_169371_length_1033_cov_4.285276, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000983 DAOREF010000000
VERSION     DAOREF010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169371_length_1033_cov_4.285276"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..833)
                     /locus_tag="PKI33_16210"
     CDS             complement(165..833)
                     /locus_tag="PKI33_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNM38617.1"
                     /translation="MQSKIRVAILDDHPMTVEGYLAKLGKDPNIEVTATIGVADLLEP
                     VLEKSEVNVLILDVTVPISSENRNSYPILHVIPTLLDEHPNLNILVVSMRVERALIRG
                     VMDAGANGYILKDDQNANLNFLEIVKLVAEGGVYLSEEAGKVYDKQLEKENGKQISPR
                     QLEALSLCAAYPGDKSMLLATKMHITHSAFRNLLSGAYIKLGARNRTEAIAKAKQMGL
                     ITPD"
BASE COUNT          272 a          229 c          223 g          309 t
ORIGIN      
        1 agcaaagttt gaaaggaggg gcttatcgta gataagtata tctagtttta agtgtactgt
       61 atattgtcaa ggcaacaaag tacagataaa cctctcagca tttggagcag cgaggttgaa
      121 acggatattg gaactgttgg attgtgcaag ttcgcgcttc agacttaatc tggcgtgatc
      181 agccccattt gctttgcctt cgcgatggct tctgtccgat tccttgcccc caatttgatg
      241 tacgccccag ataataaatt tcgaaatgca gagtgggtaa tgtgcatctt tgtagcgagc
      301 aacatgcttt tatcaccggg gtaggcagca cataacgaaa gcgcctctaa ttgacgcgga
      361 ctgatttgct tgccattttc tttttctaat tgtttgtcgt agaccttgcc cgcctcttca
      421 cttaagtaaa caccaccttc tgccactaat ttgacgatct ctaaaaaatt gaggttggca
      481 ttctggtcat ctttgagaat atatccattc gccccagcat ccattacccc gcggatcaat
      541 gcccgctcca cccgcatgct gacgactaaa atgttcaagt tcgggtgttc atctaacaat
      601 gtagggatga catgcaaaat gggatacgaa ttacggttct ccgatgagat tggaacggtc
      661 acatccagaa taaggacatt tacttcgctt ttttcgagaa ccggctctaa taaatctgcc
      721 actcctatgg ttgcggtcac ttcaatgttg gggtctttgc caagtttggc aagataccct
      781 tccacggtca tggggtgatc gtccaggatg gcaacgcgta tttttgattg catgttcacc
      841 gctcccactg catcgtcctt ctctcaaaga ttgttgacta ttgctcatta ataaaaaggg
      901 taacagaaag agaattctgt ttcaagcaat acagtacaaa tgcctgcctt ctggttattg
      961 actgtacttg tagagtctat acaataaaaa aggtttggaa tacgtttggt gtcttccaaa
     1021 tccatagaac atg
//
LOCUS       DAOREF010000984         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_169383_length_1033_cov_3.448875, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000984 DAOREF010000000
VERSION     DAOREF010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169383_length_1033_cov_3.448875"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PKI33_16215"
     CDS             <1..831
                     /locus_tag="PKI33_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNM38618.1"
                     /translation="WWKGYKLKSVENFIAEFKDKTISVALQDLETGMEFFSHADVMMH
                     PASTMKIPVMMEVFRQAEAGLFTLDDRLEIYNSFTSIADGSKFSLDVSDDSESTLYQR
                     MGESETIRELTRLMIVRSSNLASNLLMDKVSIARVDAIVKEYGIENMSIIRELEDKKA
                     LSLNMNNAACARSSTQMLRLIAEGKIVSQKACDEMIRIFLGQEFNESIPAGLPNDVKV
                     AHKTGWSGEFFHDVGIVYPPNRKPYILSLFTHGFPENKDYEAHQCMAQISKLIYKEIL
                     "
     gene            828..>1033
                     /locus_tag="PKI33_16220"
     CDS             828..>1033
                     /locus_tag="PKI33_16220"
                     /inference="COORDINATES: protein motif:HMM:NF015441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNM38619.1"
                     /translation="MITVSSLTYYPIKACRGFDVPTSNIQRMGLEHDRRMMVITPDGE
                     FLTQREYPRLALVTPTLRNEAVTLS"
BASE COUNT          289 a          235 c          240 g          269 t
ORIGIN      
        1 tggtggaagg gctataaatt gaagtctgtc gaaaatttta ttgccgaatt caaagacaaa
       61 accatcagcg ttgctttgca agatctagaa acaggcatgg agttttttag tcatgccgat
      121 gtaatgatgc accctgccag taccatgaaa attccagtga tgatggaagt attccgtcag
      181 gcagaggcag ggctgtttac gcttgatgat cgcttagaaa tttacaactc attcacaagt
      241 attgctgatg gaagtaaatt ttcgcttgac gtatcggatg attcagaatc aacactttat
      301 cagcgcatgg gagagagcga aacgattcgc gaactaaccc gtttgatgat cgtgcgttct
      361 tccaacctcg ccagcaatct gctcatggat aaggtctcaa tagcgcgagt ggacgccatt
      421 gtaaaagagt atggcatcga aaatatgtct atcatccgcg agctggaaga caaaaaagcg
      481 ttgagcctga acatgaacaa tgccgcctgt gcgcgcagtt ccacgcagat gctacgattg
      541 atcgctgaag gcaaaatcgt ttcacaaaaa gcttgcgatg aaatgattcg cattttcctt
      601 gggcaggaat tcaacgaaag tattcctgca ggtttgccaa acgatgtaaa ggtcgcgcat
      661 aaaacgggtt ggtctggcga gttctttcac gatgttggca ttgtctaccc gccgaatcgc
      721 aaaccttata tcctttcgtt attcacacat ggcttcccag aaaataaaga ttacgaagcc
      781 catcagtgta tggctcaaat ttcaaaactt atttacaaag agattctatg attactgttt
      841 cctcactcac ttactatccc atcaaagcct gccgtgggtt cgatgtgccc acgagcaaca
      901 ttcaacgcat ggggcttgaa catgaccggc gcatgatggt catcacgccg gatggcgagt
      961 tcctgactca gcgtgaatat ccgcgtctgg cgctggtcac acccaccctt aggaatgagg
     1021 cggtcacttt atc
//
LOCUS       DAOREF010000985         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_169572_length_1033_cov_1.776074, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000985 DAOREF010000000
VERSION     DAOREF010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169572_length_1033_cov_1.776074"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1033
                     /locus_tag="PKI33_16225"
     CDS             <1..>1033
                     /locus_tag="PKI33_16225"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNM38620.1"
                     /translation="DGRTVEVEWEQNFQEKDWYFYTNRSTLWHLRMDAEYKYLDLSTQ
                     LRDFVWYGKEQDYAWWVKLWGIFDDAGKTTNKDMESMNPPYSIEDLIASGVFLPETEL
                     AQILERLQSKKAMILQGPPGVGKTFIARKLAYALMEEVDNKRIEMVQFHQSYSYDDFV
                     RGYRPVEGKAGSFALQNGVFYEFCQRAVNDPDREYVFIIDEVNRGNLSQIFGELLMLI
                     EADKRDPEFAVPLVYRHENEPRFYIPPNLYLIGLMNVADRSLAMVDYALRRRFVFVNL
                     TPQYESDLYRQWLLERSMQPELVNLIIERMSALNKQVKEDSLLGENYQVGHSFFCPKG
                     DNFAKLDKA"
BASE COUNT          263 a          239 c          253 g          278 t
ORIGIN      
        1 aagatggaag aacggtcgaa gtggaatggg agcaaaactt ccaagagaag gattggtatt
       61 tctataccaa tcgcagcacg ctctggcatt tacgcatgga tgcagagtat aaatatttag
      121 atttgtctac ccagctacgc gattttgttt ggtatgggaa agaacaggat tatgcctggt
      181 gggttaagct ctggggtatt ttcgatgatg caggaaaaac aacgaacaag gatatggaat
      241 ccatgaaccc gccttatagt atcgaggacc tgatcgcctc tggtgtcttt ttacccgaaa
      301 ctgagcttgc tcaaatctta gaacgcttgc aatcgaagaa ggcgatgatt ctgcaaggtc
      361 cgcccggagt gggcaagacc ttcattgccc gcaaactcgc ttatgccttg atggaagaag
      421 tggataacaa acgcatcgaa atggtgcagt tccatcaatc gtattcctat gacgattttg
      481 tgcgcggcta tcgtcctgta gagggaaagg cgggcagctt cgctttgcag aacggcgtct
      541 tctatgagtt ctgtcaacgc gccgtcaacg acccagaccg cgaatatgtt ttcatcattg
      601 atgaagtcaa tcgcggcaat ctcagccaaa tttttggcga gttgctcatg ttaatcgaag
      661 ccgataaacg cgaccccgaa tttgcagttc ctttagttta tcgccacgag aacgagccgc
      721 gtttttacat cccacccaat ctttatctca tcggcttgat gaacgttgcc gatcgttcgc
      781 tcgccatggt cgattatgcg ttgcgtcgtc gctttgtgtt cgtcaatctc acgccgcaat
      841 acgaaagtga tctttatcgt cagtggttgc tcgaacgctc catgcaacct gagttggtta
      901 atctcatcat tgagcgcatg tccgcgctca acaagcaggt caaagaagac tctttgctcg
      961 gcgaaaatta tcaggtcggt cacagcttct tctgccctaa gggtgataac ttcgctaaat
     1021 tagataaggc ttg
//
LOCUS       DAOREF010000986         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_171758_length_1026_cov_2.832132, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000986 DAOREF010000000
VERSION     DAOREF010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171758_length_1026_cov_2.832132"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1026)
                     /locus_tag="PKI33_16230"
     CDS             complement(<1..>1026)
                     /locus_tag="PKI33_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNM38621.1"
                     /translation="FTPNRRKFSTDIKWNLYDKDVLPMWVADMDFSAPKPILDALRKQ
                     VEHGVYGYEWAGKQLPAIVAKRMDDLYKWKVQPEAVLAVTGIVSGFSVAVRAFCSPKK
                     GVAIQTPVYNEFHEVKNNIGIPQIDIPLVKNIKGNILSYEIDWDLFEKRVKKAGIFLL
                     CNPHNPLGIIFSRKELIRMAEICIKHNVIIVSDEIHSELLLDGNKFTPMAKLSREIEQ
                     HVITLVAPSKTFNIAGLFCGFAIIPNKELRDRYNTELNHLRLHVASPGLHASMVAFSG
                     KCDSWLKDLNQHLTNNRDYLVDYVTKFMPGVRTTIPVATYLGWLDFTQSDIEGSPFEF
                     FLKNARV"
BASE COUNT          292 a          244 c          211 g          279 t
ORIGIN      
        1 ccactctggc attcttcagg aagaattcaa agggtgaccc ttcaatgtcg ctttgggtaa
       61 agtctaacca gccgaggtaa gtagcaaccg gaatggtcgt gcgaacgccc ggcatgaatt
      121 tggtgacgta atctaccaaa taatctcgat tattagtgag gtgttgattc aaatctttca
      181 gccagctatc gcacttccca gagaaagcaa ccatagatgc gtgtaagccc gggctggcaa
      241 catgcaagcg caaatggttt aactcagtat tgtagcggtc gcgcaattct ttattaggaa
      301 taatggcaaa cccacagaac aaaccagcaa tattgaaggt cttggaagga gcaaccaagg
      361 taatgacatg ttgctcgatc tcacgcgaaa gtttcgccat cggcgtaaat ttattaccgt
      421 ctaagagaag ctcagaatgg atttcatctg aaacaatgat gacgttatgt ttgatgcaaa
      481 tttctgccat gcggattaat tctttacgcg agaaaataat ccccaatggg ttatgcggat
      541 tacacaacag gaaaataccc gctttcttca cgcgtttttc gaacaagtcc caatcgatct
      601 cataactcaa aatatttcct ttgatatttt tcaccaaagg aatatcaatt tgcggaatgc
      661 caatattgtt tttcacttca tgaaattcgt tataaaccgg ggtttgaatc gccacacctt
      721 tcttcggcga acaaaacgca cgcactgcca cactgaagcc actcacaatg cctgtcactg
      781 ccaacaccgc ctccggctgg actttccatt tatagagatc gtccattcgt ttggcaacaa
      841 ttgccggtaa ttgtttaccc gcccattcgt atccatacac gccatgctct acttgcttcc
      901 gcaatgcatc caaaattggt ttgggtgcag agaagtccat gtctgccacc cacatgggca
      961 acacatcttt atcgtataaa ttccacttga tatctgtaga gaacttgcgg cggtttgggg
     1021 tgaaat
//
LOCUS       DAOREF010000987         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_172102_length_1025_cov_3.841237, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000987 DAOREF010000000
VERSION     DAOREF010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172102_length_1025_cov_3.841237"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..918
                     /locus_tag="PKI33_16235"
     CDS             103..918
                     /locus_tag="PKI33_16235"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNM38622.1"
                     /translation="MSKAILVLSDALRYDVAISNMGYLGHMLEYKKASLYKIVGELPS
                     MSRPMYETIHTGMPSSETGIVANSIVRLSEKPNIFKSVREAGKVTAAAAYYWYSELYN
                     RAPYNPIDDREVDDASLNIQHGRFYTTDEYPDIELFNTAGHLVRKYSPDYLLLHPMGM
                     DYHGETYGSDTKEYRNHAIYQDSKLAPLLVEWRERGYTIFVTGDHGINADGGHGGTTP
                     EQRTVPFFVIQPNGQGRGDTGEVLSHLQIAPTVLSLLGISIPETMKHAPLALG"
BASE COUNT          262 a          233 c          251 g          279 t
ORIGIN      
        1 aaagaacagg tcacctgttc gaaagaagcg gttctggttc tggacgaata aaaaaattgt
       61 aggtcacgct caaagcgtga cctacttctt tatagaggtt ttatgagcaa agctatcctt
      121 gttctttctg atgccctgcg ttacgacgta gcgatttcca acatgggcta tttaggtcac
      181 atgttggaat ataaaaaagc aagcttgtac aagatcgttg gtgaactgcc aagcatgtct
      241 cgcccgatgt atgagaccat ccataccggt atgccttcta gtgaaactgg cattgttgcg
      301 aattcaattg tgaggctttc tgaaaaaccg aatattttta agtctgtacg agaagcaggg
      361 aaggtgacag ccgctgcagc gtattattgg tattctgagt tgtacaatcg cgcgccttac
      421 aatcccattg acgataggga agtggatgat gcttccctaa acattcagca tgggcgtttc
      481 tataccaccg acgaatatcc agatatcgag ctgttcaaca cggcgggaca tctcgtccgc
      541 aaatattcgc cagattatct tttgcttcac cctatgggca tggactatca tggcgagacc
      601 tatggttctg atactaagga gtatcgtaac catgccatct atcaagattc aaaactcgcg
      661 cctcttctcg tggaatggcg cgagcgtgga tacacaattt ttgtaacggg tgaccatggc
      721 atcaatgctg atggggggca tggcggcacc acgcccgagc agaggactgt tccatttttt
      781 gtgattcagc ccaacggtca gggaagaggc gatacgggcg aggtcctctc tcatttgcag
      841 atcgcaccca cagtgttaag tctgctgggt atttccatcc ctgaaacgat gaagcacgct
      901 cccttggctt tgggttaata acggcttctt aacacactgt tcctgcgcca ttaagatgag
      961 ccgggtattg tatagtggtt ggtcaatttt ggaggcacaa ccatgaaact ctattacggc
     1021 ttcgc
//
LOCUS       DAOREF010000988         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_172440_length_1024_cov_3.842105, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000988 DAOREF010000000
VERSION     DAOREF010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172440_length_1024_cov_3.842105"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKI33_16240"
     CDS             <1..285
                     /locus_tag="PKI33_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38623.1"
                     /translation="AMTQPLFGYDTALPLDEGLYRLLTLVTLQEGVKRGLLVHASGGV
                     GKFKKVRGGESVTEYNAVYTKHLPKRRQLPWTLIGRISDVMIPYFKKMDF"
     gene            complement(301..>1024)
                     /locus_tag="PKI33_16245"
     CDS             complement(301..>1024)
                     /locus_tag="PKI33_16245"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNM38624.1"
                     /translation="RLKPLDGDGQHCISFQLTTEPKDIVLRYTDHFGNDVRHFDVIQP
                     HQKLMASAISEVITPDVYQSGSSNLSPLDEFNYLSPTHYAPHSPDLNLFAASLKTSDS
                     ALENSLKVMHTLFTTLTYEKGATDVNTTADKALLLGRGVCQDFSHIMLAVCRLQGIPA
                     RYVSGYLYNNGHTAASHAWVDVLTPERGWVSLDPTHDREQTEQYVRVAVGRDYADVPP
                     TRGIFKGNAKEQMDVNVSVKSL"
BASE COUNT          245 a          210 c          316 g          253 t
ORIGIN      
        1 gccatgaccc agcctttgtt cggctatgac acagcgcttc ctttggatga aggcttgtat
       61 cgcttgttga cgttggtcac attacaagag ggtgtgaagc gcggattgct tgtgcatgcc
      121 agcggtgggg tgggtaagtt caaaaaagta cgcggcggcg agtctgtgac ggagtacaac
      181 gcggtataca cgaagcacct gccgaagcgc cgtcaactgc cgtggacgtt gatcggcaga
      241 atttcggatg tgatgattcc gtattttaag aagatggatt tctagaattc ttttggagga
      301 ctagagggat ttcacagaga cgtttacgtc catttgttct ttggcgttgc ctttgaaaat
      361 accgcgtgtg gggggcacgt ctgcataatc gcgcccgaca gccacacgca catattgctc
      421 ggtttgttca cggtcgtgag ttgggtcaag ggagacccag ccacgttcgg gggttagaac
      481 atcgacccag gcgtgagagg cggcggtgtg accattattg tataagtagc cgctcacata
      541 gcgggcaggg atgccctgca agcggcaaac cgcaagcatg atgtgtgaaa agtcctgaca
      601 aactccgcga cccagcaata aggctttgtc ggcagtggtg ttgacatctg tagcgccttt
      661 ttcataggtg agagttgtaa acagggtgtg cataacctta agcgaatttt ccaaggcgga
      721 gtcggatgtt tttagggaag cagcgaaaag gttcaggtca ggggaatgag gcgcgtaatg
      781 agtgggtgaa aggtaattga actcatccaa aggcgagagg ttggatgagc ccgactggta
      841 gacatctggt gtgatgactt cactaatggc agatgccatc aatttttgat gcggttggat
      901 cacgtcaaaa tggcggacgt cattgccgaa atggtctgta tagcgcagaa caatatcttt
      961 gggttcggtg gttaattgga acgagataca gtgctgacca tctccgtcga ggggtttgag
     1021 acgc
//
LOCUS       DAOREF010000989         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_174005_length_1020_cov_1.948187, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000989 DAOREF010000000
VERSION     DAOREF010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174005_length_1020_cov_1.948187"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKI33_16250"
     CDS             complement(<1..795)
                     /locus_tag="PKI33_16250"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNM38625.1"
                     /translation="MTNIWWIRRDLRLTDNAALHSALESGSILPVFILDPAFDNSSER
                     RKHFLYEGLSSLDADLRKRGSYLVIRKGKPADTLRILFDEVKAETVYAEEDYTPYSHK
                     RDKLIQKLLPLQLIQGQTVHHPKSVLKQDGRPYTVYTPYSKVWKAQLAKIKLHPAPEH
                     INSPKDIRSEALPAFKTNPIFPASEAEALRRLDQFTKRAITNYHDDRNRMDLDGTSSL
                     SPYIRFGMIGLRQAVHAARSTQHADSKGAEIWLNELIWREFYIQILY"
     gene            complement(792..>1020)
                     /locus_tag="PKI33_16255"
     CDS             complement(792..>1020)
                     /locus_tag="PKI33_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38626.1"
                     /translation="FTGTLTSELALSLFGNTKIRAYGSLDFKDNAGNTGHLELNRQGY
                     VTLKINDQPTVTHALVRVMWLDMMNLSPQPL"
BASE COUNT          251 a          213 c          279 g          277 t
ORIGIN      
        1 gtacaagatc tgaatgtaga actcgcgcca aataagctcg ttgagccaga tttctgcacc
       61 tttcgagtct gcgtgctgtg tactacgtgc cgcatgtacg gcttgacgca acccaatcat
      121 cccgaagcgg atataaggtg agagcgatga tgttccatcc aaatccatgc ggttgcggtc
      181 gtcatggtaa tttgtgatcg ctcgtttggt aaattgatcg agacgacgta acgcttcagc
      241 ctcactagcc gggaaaatag ggtttgtctt gaatgcaggc agtgcctcag aacgaatatc
      301 ttttggcgag ttgatatgct ctggtgcagg atgtaacttg attttggcta attgagcctt
      361 ccatactttg gaatatggtg tgtataccgt atagggtctg ccatcttgtt tgagaacaga
      421 tttggggtga tgaacggttt gcccctgaat taattgcaat ggcagcagtt tttggatcag
      481 cttatcccgt ttatgggaat acggtgtgta atcttcttct gcataaacgg tttccgcttt
      541 aacttcgtca aagagtattc gcaatgtatc tgccggtttg cccttgcgaa tcacaaggta
      601 agaccctcgt ttgcgcaggt ctgcatctaa ggaagataag ccttcgtaga gaaaatgttt
      661 tcgccgttca gatgagttgt cgaatgctgg gtcgagaata aagactggca gaatagaacc
      721 agactcgagc gcggaatgaa gcgcagcatt gtccgttaaa cgaaggtcac ggcgaatcca
      781 ccaaatattt gtcatagcgg ttgcggagaa aggttcatca tatccagcca catcacacgc
      841 acaagcgcat gtgtgacggt aggctggtcg ttgattttta gggtgacgta gccctgacgg
      901 ttgagctcaa gatgcccagt gttgccggca ttgtctttga aatcgaggga gccataggcg
      961 cggatcttgg tattgccgaa aagggaaagg gcaagctcgc tagtgagcgt gccggtgaat
//
LOCUS       DAOREF010000990         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_177927_length_1009_cov_2.031447, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000990 DAOREF010000000
VERSION     DAOREF010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177927_length_1009_cov_2.031447"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKI33_16260"
     CDS             <1..655
                     /locus_tag="PKI33_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNM38627.1"
                     /translation="KEFEQDPQAEVWLYLDVHQIVHYQKRHEQQEMALHDMLLTKRPK
                     ISLPPSTLEYGVSIAASLAHYFLRQRRSVGFASAGQTFTVLPAERSERQEAKILETLA
                     FVKADGILSLAGLVATQAAVLPQGSTVILITPTIRSDLLQAVDDLVYHYLSPVIVLLN
                     AKSFEGPSGTAELVSSLKARRVPVITISCDDDLPQALADISSNFKLQELHAWRTPLS"
     gene            634..>1009
                     /locus_tag="PKI33_16265"
     CDS             634..>1009
                     /locus_tag="PKI33_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNM38628.1"
                     /translation="MANTPIVTIDLHFQGKTQAIASYLIRQGDAVVLIESGPGSTLSA
                     LEAGLAKEGLSPRDITHVLLTHIHLDHAGAAGWLSRQGAEIYVHPIGAPHLLNPEKLI
                     ASATRIYGDRMDTLWGEFLPVAQ"
BASE COUNT          251 a          258 c          251 g          249 t
ORIGIN      
        1 gaaggaattt gagcaagacc cacaagctga agtttggctt tatctcgatg tgcatcaaat
       61 agtacattat caaaagcggc atgaacagca agaaatggcg ttgcacgaca tgctattaac
      121 caaacgcccc aagataagtt tgccgccatc tacattggag tatggtgtaa gtattgccgc
      181 atcgctagcg cattatttcc ttcgtcaacg ccgctcggta gggtttgcca gcgccgggca
      241 gacctttact gttttgcccg cagaacgcag tgaaagacag gaagcgaaga tcctagaaac
      301 gctggcattc gtcaaagcag atggcatcct ttcgttggct ggactggtgg caacacaagc
      361 ggcggttttg ccgcaaggat caacggtcat tctcatcacg ccaaccattc gttcagacct
      421 tttgcaagcc gtggatgatc tggtgtatca ctatctttct ccggtgatcg tattattaaa
      481 tgcaaagtca tttgaaggtc catctggaac cgcagaactg gtatcatcac tcaaagcacg
      541 gcgcgtgccg gtcatcacca tttcatgtga cgatgatcta ccgcaggcgc tggcagacat
      601 atcctctaat ttcaaactac aggaattgca tgcatggcga acacccctat cgtaacgatt
      661 gacctccatt ttcaaggcaa gactcaagcc atcgcctcgt atttgatacg acaaggcgat
      721 gctgttgtct taatcgaaag cggacccggc tcgactcttt ctgcgctcga agctggtttg
      781 gcgaaagagg gcttatctcc ccgagacata acccacgttc tgctcacaca cattcatctt
      841 gaccatgcag gggcggcggg ttggttgtct cgtcagggcg cggagattta tgtacacccc
      901 attggggctc cgcatttatt gaaccccgag aagctgatag ccagtgccac ccgtatttat
      961 ggagatagga tggatacact ctggggtgag tttctgcccg tagcgcagg
//
LOCUS       DAOREF010000991         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0861
            NODE_180066_length_1003_cov_3.210970, whole genome shotgun
            sequence.
ACCESSION   DAOREF010000991 DAOREF010000000
VERSION     DAOREF010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852204
            Sequence Read Archive: SRR3501887
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:23:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,255
            CDSs (total)                      :: 3,235
            Genes (coding)                    :: 3,225
            CDSs (with protein)               :: 3,225
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180066_length_1003_cov_3.210970"
                     /isolate="AMR_MDS_0861"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PKI33_16270"
     CDS             complement(<1..305)
                     /locus_tag="PKI33_16270"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNM38629.1"
                     /translation="MTDNWNLLGHEWAVDMLRRHVARGEMRHAYLFAGAPGLGRRTLA
                     LRLAQALNCEKPIAPGEPCFVCRTCKQIDAMQHPDLAIIQAVDEDGVGKDGGTLRVD"
     gene            complement(384..>1003)
                     /gene="rsgA"
                     /locus_tag="PKI33_16275"
     CDS             complement(384..>1003)
                     /gene="rsgA"
                     /locus_tag="PKI33_16275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNM38630.1"
                     /translation="VFVFACANPNPKLRMLDRFLVIAEKQKLPVVIVANKIDLVANPK
                     DSFGMYEAIGYHVIYTSTKSETGLIELRNQLRNKLSAFAGPSGVGKSSLLNALQPGLG
                     LAVNEISSVMNKGRHTTVVRQMFAFEGGYVADTPGWKSLGLWDTEPEEIDAYFPELRG
                     LVEQCQFSDCTHQHEPGCAVLTALKDGKIHAERYESFVRLRSGKE"
BASE COUNT          261 a          266 c          224 g          252 t
ORIGIN      
        1 tgatccacgc gcaaggtgcc gccatccttc cccactccat cttcgtctac ggcttgaatg
       61 atcgctaaat ctgggtgttg catggcgtcg atctgtttgc aagtgcggca cacaaaacaa
      121 ggctctcccg gcgcaatggg tttctcacag ttcaaagctt gtgctaggcg caatgccaac
      181 gttcggcgac ccaaacccgg agcgcccgca aaaagataag catgacgcat ctctccgcgc
      241 gctacatggc ggcgaagcat atcgactgcc cattcgtgcc cgagtaaatt ccagttatca
      301 gtcattggtt taagtatcag gggtcagaga ttaagtgtca agtgtggaaa gcatgcaata
      361 cgtatttggt aattttctac gcgttattct ttgccagaac ggagccttac aaaagactca
      421 tatcgttctg catgaatctt gccatctttc aatgctgtga gcacggcaca acccggctca
      481 tgttgatggg tgcaatcact aaattgacat tgctccacca acccacgcaa ctcagggaag
      541 taagcgtcga tctcttcagg ttcggtatcc cacaagccaa gagacttcca accgggtgta
      601 tctgcgacat agccaccttc aaaggcaaac atttggcgca ccaccgtggt atgacgtccc
      661 ttattcatca cagaactaat ttcgttcaca gccaaaccca aaccaggctg taaagcgttc
      721 aataaactcg acttgcccac accgctgggt ccggcaaagg cgctgagctt gttccgcaat
      781 tgattgcgaa gttctataag acccgtctcg cttttcgtgg atgtataaat tacgtgatag
      841 ccaatagctt cgtacattcc aaaactatct tttggattcg caacgaggtc aatcttattc
      901 gccacgatta ccacaggaag tttttgcttt tctgcaataa caagaaaccg gtcgagcatt
      961 ctcaatttgg gatttggatt cgcgcatgcg aagacgaaga cag
//
